Halothane metabolism: Kupffer cells carry and partially process trifluoroacetylated protein adducts.
Kupffer cells, prepared 18 h after pretreatment of rats with a single dose of halothane, did carry TFA-adducts which were recognized on Western blots by a anti-TFA-antibody. Based on apparent molecular weight, the pattern of the major TFA-adducts within Kupffer cells was similar to that observed in hepatocytes. When kept in primary culture, Kupffer cells processed TFA-adducts of apparent molecular weight of 220 kD, 110 kD and 74 kD within 24 or 48 h; in contrast, other TFA-adducts were persistent for at least 48 h in Kupffer cells. The data suggest a role for Kupffer cells in processing of chemically altered proteins in the liver.
Christen
U
U
Dept. of Pharmacology, Biocenter of the University, Basel, Switzerland.
Bürgin
M
M
Gut
J
J
D000818:Animals; D001711:Biotransformation; D015153:Blotting, Western; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D006221:Halothane / Q000378:metabolism*; D007728:Kupffer Cells / Q000166:cytology / Q000378:metabolism*; D008099:Liver / Q000166:cytology; D008297:Male; D008970:Molecular Weight; D011485:Protein Binding; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D011506:Proteins; D006221:Halothane