Human immunodeficiency virus infection of human-PBL-SCID mice.
Severe combined immunodeficient (SCID) mice reconstituted with human peripheral blood leukocytes (hu-PBL-SCID mice) have inducible human immune function and may be useful as a small animal model for acquired immunodeficiency syndrome (AIDS) research. Hu-PBL-SCID mice infected with human immunodeficiency virus-1 (HIV-1) contained virus that was recoverable by culture from the peritoneal cavity, spleen, peripheral blood, and lymph nodes for up to 16 weeks after infection; viral sequences were also detected by in situ hybridization and by amplification with the polymerase chain reaction (PCR). Mice could be infected with multiple strains of HIV-1, including LAV-1/Bru, IIIB, MN, SF2, and SF13. HIV-1 infection affected the concentration of human immunoglobulin and the number of CD4+ T cells in the mice. These results support the use of the hu-PBL-SCID mouse for studies of the pathogenesis and treatment of AIDS.
Mosier
D E
DE
Division of Immunology, Medical Biology Institute, La Jolla, CA 92037.
Gulizia
R J
RJ
Baird
S M
SM
Wilson
D B
DB
Spector
D H
DH
Spector
S A
SA
D000818:Animals; D001803:Blood Transfusion; D002678:Chimera / Q000276:immunology*; D004195:Disease Models, Animal; D015658:HIV Infections / Q000276:immunology; D015497:HIV-1 / Q000302:isolation & purification; D006801:Humans; D007153:Immunologic Deficiency Syndromes / Q000235:genetics / Q000276:immunology*; D017710:Lymphocyte Transfusion; D051379:Mice; D008817:Mice, Mutant Strains / Q000276:immunology*; D013154:Spleen / Q000382:microbiology