title: The influence of artificial colonization with E. coli strain O83 on the intestinal flora in infants.
abstract: Dominant bacterial strains present in stool (with particular emphasis on E. coli strains) were examined in 4 groups of healthy infants: breast-fed and bottle-fed, colonized with E. coli O83, and control (non-colonized) breast-fed and bottle-fed newborns. The presence of fimbriae was examined by hemagglutination, the P-fimbriae-bearing strains were tested by the PPA latex test. In addition, adherence to cell line HT-29 and serotyping was performed in selected strains. The E. coli strain O83 was found to possess type 1 fimbriae. Fewer bacterial strains possessing type 1 fimbriae were found in E. coli O83-colonized infants (except the O83 serotype) than in control infants. The E. coli O83 strain colonized significantly better the breast-fed than the bottle-fed infants; its higher adherence activity was demonstrated even in cell line HT-29. Finally, colonization with E. coli O83 influenced the character of microbial intestinal flora: the frequency of positive E. coli isolates was significantly higher in colonized (both breast- and bottle-fed) than noncolonized infants.
authors.affiliation: Institute for the Care of Mother and Child, Prague.
authors.forename: M
authors.initials: M
authors.lastname: Slavíková
authors.forename: R
authors.initials: R
authors.lastname: Lodinová-Zádníková
authors.forename: L A
authors.initials: LA
authors.lastname: Hanson
authors.forename: I
authors.initials: I
authors.lastname: Adlerberth
authors.forename: B
authors.initials: B
authors.lastname: Carlsson
authors.forename: A E
authors.initials: AE
authors.lastname: Wold
authors.forename: C
authors.initials: C
authors.lastname: Scanborg Edén
mesh_terms: D001422:Bacterial Adhesion; D001903:Bottle Feeding; D001942:Breast Feeding; D002460:Cell Line; D004926:Escherichia coli / Q000145:classification / Q000254:growth & development* / Q000502:physiology; D010861:Fimbriae, Bacterial; D006090:Gram-Negative Bacteria / Q000254:growth & development*; D006386:Hemagglutination Tests; D006801:Humans; D007231:Infant, Newborn; D007422:Intestines / Q000382:microbiology*; D012703:Serotyping