title: Sequence analysis of the gene encoding the Chlamydia pneumoniae DnaK protein homolog.
abstract: The antigen-coding region of a 4.2-kb PstI fragment of Chlamydia pneumoniae (pLC3), which encodes a 75-kDa immunoreactive protein recognized during human C. pneumoniae infection, was localized to a 2.0-kb EcoRI fragment. This subclone expressed an immunoreactive fusion protein of ca. 82 kDa. Nucleotide sequence analysis of the C. pneumoniae gene revealed that it consisted of a 1,980-base open reading frame with an inferred 71,550-Da protein of 660 amino acids. Putative Escherichia coli-like promoters and a ribosomal binding site were located in the 5' upstream region, and an 11-base dyad forming a stable stem-loop structure following two in-frame stop codons was identified. The C. pneumoniae 75-kDa protein is a member of the hsp70 family of heat shock proteins and has 87% amino acid similarity with the Chlamydia trachomatis protein.
authors.affiliation: Department of Pathobiology, University of Washington, Seattle 98195.
authors.forename: J M
authors.initials: JM
authors.lastname: Kornak
authors.forename: C C
authors.initials: CC
authors.lastname: Kuo
authors.forename: L A
authors.initials: LA
authors.lastname: Campbell
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D002689:Chlamydia / Q000235:genetics*; D002692:Chlamydia trachomatis / Q000235:genetics; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C063842:dnaK protein, E coli