Evaluation of key gluconeogenic enzymes in experimental biliary obstruction.
In order to evaluate the usefulness of key gluconeogenic enzymes, in relation to the markers commonly used (alkaline phosphatase and gamma-glutamyl transpeptidase) for the diagnose of cholestasis the serum activity of phosphoenolpyruvate carboxykinase, fructose 1,6-bisphosphatase and glucose-6-phosphatase has been measured in rats with bile-duct ligation. Among the gluconeogenic enzymes studied only phosphoenolpyruvate carboxykinase activity increased significantly in the first 48 hours after cholestasis, decreasing thereafter to normal values. Both alkaline phosphatase and gamma-glutamyl transpeptidase activities showed a very significant increase which persisted throughout the experiment. These results seem to indicate that in spite of the high organ specificity of these enzymes they do not appear to be useful for the diagnosis of cholestasis.
Morata
P
P
Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad de Málaga, Spain.
Colmenero
J D
JD
Alonso
A
A
García-Caballero
M
M
D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001652:Bile Ducts / Q000601:surgery; D001663:Bilirubin / Q000097:blood; D015415:Biomarkers / Q000097:blood; D002779:Cholestasis / Q000201:enzymology*; D005260:Female; D006597:Fructose-Bisphosphatase / Q000097:blood*; D005943:Gluconeogenesis; D005954:Glucosephosphate Dehydrogenase / Q000097:blood*; D008026:Ligation; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000097:blood*; D051381:Rats; D011919:Rats, Inbred Strains; D005723:gamma-Glutamyltransferase / Q000097:blood
D015415:Biomarkers; D005954:Glucosephosphate Dehydrogenase; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D006597:Fructose-Bisphosphatase; D010729:Phosphoenolpyruvate Carboxykinase (GTP); D001663:Bilirubin