Involvement of lymphocyte function-associated antigen-1 (LFA-1) but not ICAM-1 in a radioactive leukocyte cell-mediated immunity (LA-CMI) assay.
A number of monoclonal antibodies (Mab) specific to human leukocyte adherence molecules were investigated for their possible function in LA-CMI assay. These data indicate that LFA-1 appears to be directly involved in LA-CMI of human peripheral blood leukocytes since both MAb H52, specific to LFA-1 beta and MAb MHM24, specific to LA-1 alpha inhibited LA-CMI that retained distinct functional characteristics normally exhibited in LFA-1-dependent adhesion steps. That is, binding of cells onto tube wall required the presence of bivalent cations and metabolic energy production; it could be stimulated by phytohemagglutinin (PHA) and was partially dependent on intact cytoskeleton and temperature. MAbs specific to other adherence molecules such as ICAM-1, CD2, CD58, CD4, CD8, CD11b and LAM-1 did not affect leukocyte adherence in this model.
Fekete
B
B
Department of Neoplastic Disease, Mount Sinai School of Medicine, New York, NY 10029.
Hildreth
J E
JE
Holland
J F
JF
Bekesi
J G
JG
D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000502:physiology; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000276:immunology / Q000502:physiology*; D003601:Cytotoxicity Tests, Immunologic; D006801:Humans; D007111:Immunity, Cellular; D018799:Intercellular Adhesion Molecule-1; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology / Q000502:physiology*; D011137:Polystyrenes
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D016169:Lymphocyte Function-Associated Antigen-1; D011137:Polystyrenes; D018799:Intercellular Adhesion Molecule-1