Effect of L-methionine on 2-carboxybenzaldehyde reductase induction by phenobarbital in primary cultures of rat hepatocytes.
Effects of phenobarbital (PB) and L-methionine on 2-carboxybenzaldehyde (CBA) reductase in rat hepatocyte primary culture were examined. Inclusion of PB in the culture medium markedly enhanced the CBA reductase activity while L-methionine, which elevates the cellular glutathione (GSH) level, suppressed the stimulatory effect of PB. This suppression, though less pronounced, was also found with other precursors of GSH biosynthesis. GSH-depletors, buthionine sulfoximine (BSO) or diethylmaleate (DEM), enhanced the CBA reductase activity suggesting that GSH plays an important role in enzyme induction.
Tonda
K
K
Shionogi Research Laboratories, Shionogi and Co., Ltd., Osaka, Japan.
Hirata
M
M
D000429:Alcohol Oxidoreductases / Q000096:biosynthesis*; D000818:Animals; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003540:Cystathionine / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D005978:Glutathione / Q000502:physiology; D066298:In Vitro Techniques; D008297:Male; D008715:Methionine / Q000494:pharmacology*; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D003609:Dactinomycin; D003540:Cystathionine; D003513:Cycloheximide; D008715:Methionine; D000429:Alcohol Oxidoreductases; C051107:2-carboxybenzaldehyde reductase; D005978:Glutathione; D010634:Phenobarbital