DNA flow cytometry in the prognosis of primary malignant melanoma.
DNA flow cytometry was carried out on 804 primary melanomas. The data were analyzed with a follow-up of 24-96 months. 57% of the cases were diploid, 32% had one abnormal cell population, and 11% were multiclonal. In 8% of the aneuploid tumors there were cell lines in the hypertetraploid range. A reliable S phase determination was possible in 524 cases. Among these 11% had an S phase exceeding 15%. Using an increased tumor thickness, relapse rate and mortality as criteria of tumor progression, aneuploidy and multiclonality, the occurrence of hypertetraploid cell lines and a high S phase (greater than 15%) proved to be correlated with a poor prognosis.
Bartkowiak
D
D
Fachklinik Hornheide, Münster, BRD.
Schumann
J
J
Otto
F J
FJ
Lippold
A
A
Drepper
H
H
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000782:Aneuploidy; D002648:Child; D002675:Child, Preschool; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D007223:Infant; D008545:Melanoma / Q000235:genetics / Q000401:mortality* / Q000473:pathology; D008875:Middle Aged; D011379:Prognosis; D016196:S Phase; D015996:Survival Rate
D004273:DNA, Neoplasm