title: Microparticle-enhanced nephelometric immunoassay (Nephelia) for immunoglobulins G, A, and M.
abstract: Covalent binding of gamma chains of IgG, whole IgA, and mu chains of IgM on polyfunctional hydrophilic microspheres (MS) yields MS-Ig conjugates, usable as reagents in new microparticle-enhanced nephelometric immunoassays (Nephelia). The principle of the assays is inhibition by free analyte (IgG, IgA, and IgM) of agglutination of the MS-Ig conjugate with specific antiserum, the light scattered by the aggregates being measured with a nephelometer. The immunoglobulin assays developed are easy to perform (single-step assays, no washing or phase separation) and sensitive (high dilution of biological samples to exclude interferences and pretreatment). Analytical recovery results (95.4-101.2%) and correlations with generally used commercial assays (r = 0.86-0.98) indicate that the assays are accurate for large concentration ranges of immunoglobulins. Precision study gives CVs = 2.8-9.6%. Nephelia appears to be useful for quantifying a large variety of biological molecules.
authors.affiliation: Immunology Laboratory, Faculty of Medicine, Vandoeuvre-les-Nancy, France.
authors.forename: M L
authors.initials: ML
authors.lastname: Cuilliere
authors.forename: P
authors.initials: P
authors.lastname: Montagne
authors.forename: T
authors.initials: T
authors.lastname: Bessou
authors.forename: R
authors.initials: R
authors.lastname: el Omari
authors.forename: D
authors.initials: D
authors.lastname: Riochet
authors.forename: P
authors.initials: P
authors.lastname: Varcin
authors.forename: P
authors.initials: P
authors.lastname: Laroche
authors.forename: P
authors.initials: P
authors.lastname: Prud'homme
authors.forename: J
authors.initials: J
authors.lastname: Marchand
authors.forename: O
authors.initials: O
authors.lastname: Flecheux
mesh_terms: D006801:Humans; D007118:Immunoassay / Q000706:statistics & numerical data; D007070:Immunoglobulin A / Q000032:analysis* / Q000134:cerebrospinal fluid; D007074:Immunoglobulin G / Q000032:analysis* / Q000134:cerebrospinal fluid; D007075:Immunoglobulin M / Q000032:analysis* / Q000134:cerebrospinal fluid; D007700:Kinetics; D008863:Microspheres; D009391:Nephelometry and Turbidimetry; D011786:Quality Control
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M