title: Transforming growth factor-alpha augments meiotic maturation of cumulus cell-enclosed mouse oocytes.
abstract: Growth factors have been shown to play an important role in the regulation of ovarian function. In this study, we examined the effects of transforming growth factor-alpha (TGF-alpha) on the meiotic maturation of immature mouse oocytes in vitro. Cumulus cell-enclosed oocytes were exposed to TGF-alpha with or without the meiotic inhibitor hypoxanthine (HX), and oocyte maturation was assessed by germinal vesicle breakdown (GVBD). Likewise, mechanically denuded oocytes were examined for GVBD following exposure to HX and TGF-alpha. When cumulus cell-enclosed oocytes were exposed to TGF-alpha (1 microgram/ml) in the presence of HX (4 mM), an increase in GVBD was observed first after 5 hours of culture. Maximal stimulation was reached at 24 hours when 70% of the oocytes underwent maturation in the presence of TGF-alpha and HX as compared to 33% with HX only. Concentrations of TGF-alpha as low as 0.1 ng/ml produced a similar stimulatory response after 24 hours of culture. Spontaneous maturation in the presence of TGF-alpha, but without HX, was also enhanced. The stimulation of GVBD by TGF-alpha showed an increase over time both with and without HX. When denuded oocytes were exposed to TGF-alpha in the presence of HX, no effect was observed. Our results suggest that TGF-alpha is a potent stimulator of mouse oocyte maturation in vitro and that its effect is mediated by the surrounding cumulus cells.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Jones Institute for Reproductive Medicine, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk 23510.
authors.forename: C
authors.initials: C
authors.lastname: Brucker
authors.forename: N J
authors.initials: NJ
authors.lastname: Alexander
authors.forename: G D
authors.initials: GD
authors.lastname: Hodgen
authors.forename: B A
authors.initials: BA
authors.lastname: Sandow
mesh_terms: D000818:Animals; D005260:Female; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000494:pharmacology; D007700:Kinetics; D008540:Meiosis; D051379:Mice; D009865:Oocytes / Q000502:physiology*; D016211:Transforming Growth Factor alpha / Q000494:pharmacology*
chemical_list: D007042:Hypoxanthines; D016211:Transforming Growth Factor alpha; D019271:Hypoxanthine