Rat DARPP-32: cloning, sequencing, and characterization of the cDNA.
DARPP-32, a substrate for cyclic AMP-dependent protein kinase, is highly enriched in the caudate nucleus. In the present study, the cDNA for rat DARPP-32 was isolated and characterized. When compared to the coding region of bovine DARPP-32 cDNA, there was 86% identity at the nucleotide level, and 84% identity at the amino acid level. The homology in the region previously noted to be similar to phosphatase inhibitor-1 remained intact. There were also 3'-untranslated regions that were highly conserved. The DARPP-32 mRNA, which appeared as a doublet, was widely distributed in the central nervous system and was highly enriched in the striatum. Southern blot analysis revealed a simple hybridization pattern, consistent with the presence of a single gene coding for rat DARPP-32. Unilateral destruction of the nigrostriatal dopaminergic pathway by injection of 6-hydroxydopamine (6-OHDA) into the substantia nigra did not alter the striatal steady state levels of DARPP-32 mRNA.
Ehrlich
M E
ME
Laboratory of Molecular and Cellular Neuroscience, Rockefeller University, New York, New York 10021.
Kurihara
T
T
Greengard
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P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D005786:Gene Expression Regulation / Q000187:drug effects; D015698:Genomic Library; D006892:Hydroxydopamines / Q000494:pharmacology; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D016627:Oxidopamine; D010750:Phosphoproteins / Q000235:genetics*; D011061:Poly A / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D013378:Substantia Nigra / Q000187:drug effects
D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D006892:Hydroxydopamines; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D011061:Poly A; D016627:Oxidopamine