title: Further evidence for a simple Cl- conductance pathway in nutrient membrane of frog stomach.
abstract: A decrease in nutrient Cl- increases the negativity of the nutrient relative to the secretory side. It seemed possible that Cl- transport could result from a neutral Cl- mechanism in the nutrient membrane coupled to a simple Cl- conductance pathway in the secretory membrane. Experiments in HCO3(-)-free, K(+)-free and Na(+)-free solutions in both bathing media gave for a 10-fold change in nutrient Cl- a PD change of 9.5 mV. Similar experiments with 0.5 mM DIDS in the nutrient solution gave for a 10-fold change in nutrient Cl- a PD change of 7.9 mV. These experiments eliminated a neutral Cl(-)-HCO3- exchanger, a NaCl and a KCl symport. Thus the change in PD could best be explained by a simple Cl- conductance in the nutrient membrane.
authors.affiliation: Department of Physics, University of Louisville, KY 40292.
authors.forename: M
authors.initials: M
authors.lastname: Schwartz
authors.forename: T L
authors.initials: TL
authors.lastname: Holloman
authors.forename: J Y
authors.initials: JY
authors.lastname: Zhang
authors.forename: G
authors.initials: G
authors.lastname: Carrasquer
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D002712:Chlorides / Q000378:metabolism*; D004553:Electric Conductivity; D005748:Gastric Fundus; D005753:Gastric Mucosa / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008564:Membrane Potentials; D011894:Rana pipiens; D013270:Stomach / Q000502:physiology*
chemical_list: D002712:Chlorides; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid