Selective age-related changes in neuronal markers and smooth muscle reactivity in cerebrovascular beds of Fischer 344 rats.
Choline accumulation, choline acetyltransferase (ChAT) and glutamic acid decarboxylase (GAD) activities were measured simultaneously in various cerebrovascular beds and brain areas from Fischer 344 rats aged 4.5 and 22 months. A slight (25%) but not significant decrease in choline accumulation was observed concomitantly with a significant increase (187%, p less than 0.05) in ChAT activity in the major cerebral arteries of the 22-month-old rats. In small cortical pial vessels and selected brain regions, cholinergic and GABAergic biochemical markers remain unaltered in aged rats. The vasomotor reactivity of the basilar artery was investigated in rats of 4.5, 12, 22 and 30 months of age. In the 22-month-old rats, maximal responses to 5-hydroxytryptamine (-25%, no significant) and prostaglandin F2 alpha (-30%, p less than 0.05 by ANOVA) were less intense as compared to other age-groups despite preserved contractile responses to dopamine, uridine triphosphate or a depolarizing concentration of K+. Relaxations induced by histamine, acetylcholine, noradrenaline, adenosine and somatostatin were strictly comparable among the different age-groups. The sensitivity of the basilar artery to all vasoactive agents failed to demonstrate any correlation with age. Our study suggests that cerebrovascular cholinergic and GABAergic markers undergo minor and selective changes with increasing age. Further, basilar artery vasomotor functions appeared relatively spared by the aging process despite age-related selective decreases in contractile responses to 5-hydroxytryptamine and prostaglandin F2 alpha.
Hamel
E
E
Cerebral Circulation and Metabolism Group, Synthélabo-Recherche (L.E.R.S.), Bagneux, France.
Assumel-Lurdin
C
C
Bouloy
M
M
MacKenzie
E T
ET
D000375:Aging / Q000502:physiology*; D000818:Animals; D001488:Basilar Artery / Q000378:metabolism; D015415:Biomarkers; D002560:Cerebrovascular Circulation / Q000502:physiology*; D002794:Choline / Q000378:metabolism; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D005968:Glutamate Decarboxylase / Q000378:metabolism; D008297:Male; D009043:Motor Activity / Q000502:physiology; D009131:Muscle, Smooth, Vascular / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000502:physiology*; D018377:Neurotransmitter Agents / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344
D015415:Biomarkers; D009419:Nerve Tissue Proteins; D018377:Neurotransmitter Agents; D002795:Choline O-Acetyltransferase; D005968:Glutamate Decarboxylase; D002794:Choline