title: Rapid analysis of carcinoembryonic antigen levels in gallbladder bile. Identification of patients at high risk of colorectal liver metastasis.
abstract: Recently it was found that immunoanalysis of carcinoembryonic antigen (CEA) levels in gallbladder bile may be a sensitive method to detect colorectal liver metastases in humans. Methods used in the past for the detection of CEA in various body fluids were cumbersome and time consuming, requiring acid extraction, extensive dialysis, and column purification. Single-step, solid-phase radioimmunoassays, designed specifically for serum CEA analysis, were developed commercially to replace these methods. Parameters and methodology necessary to adapt these kits for Parameters and methodology necessary to adapt these kits for use with gallbladder bile are presented here. A combination of pretreatment procedures for bile, before radioimmunoassay, permit rapid, reproducible, and accurate measurement of CEA levels in gallbladder bile.
authors.affiliation: Department of Surgery, JHMHC, University of Florida, College of Medicine, Gainesville 32610.
authors.forename: T J
authors.initials: TJ
authors.lastname: Yeatman
authors.forename: A K
authors.initials: AK
authors.lastname: Kimura
authors.forename: E M
authors.initials: EM
authors.lastname: Copeland
authors.forename: K I
authors.initials: KI
authors.lastname: Bland
mesh_terms: D001646:Bile / Q000276:immunology*; D002272:Carcinoembryonic Antigen / Q000032:analysis* / Q000097:blood; D015179:Colorectal Neoplasms / Q000473:pathology*; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis / Q000556:secondary*; D011863:Radioimmunoassay / Q000379:methods; D015203:Reproducibility of Results; D012307:Risk Factors; D012680:Sensitivity and Specificity
chemical_list: D002272:Carcinoembryonic Antigen