title: [Testicular morphological and biochemical changes after Fowler-Stephens orchiopexy in rats].
abstract: Fowler-Stephens orchiopexy is the most common method of treating high-positioned undescended testes. Its success rate has been reported to be as high as 50-70% based on palpation or in a few circumstances on biopsy. Although it is convenient to evaluate testicular function by palpation and/or biopsy, this method is very subjective and is not scientific enough. To determine testicular function more precisely and objectively, we performed the Fowler-Stephens orchiopexy on Sprague-Dawley rats and observed the morphological and biochemical changes, including assays of LDH, SDH and the testosterone level. In our morphological study, with the use of Fowler-Stephens orchiopexy, only 17% (3/18) of the rat tests could be salvaged. The others revealed either necrosis or fibrosis. Testicular LDH, checked at the 4th postoperative week, revealed 0.62 +/- 0.04 U/mg which was statistically different (p less than 0.05) from that of the control group and the hemicastrated group (0.77 +/- 0.05 U/mg and 0.76 +/- 0.07 U/mg, respectively). The SDH obtained from the testes also revealed significant differences between the study group and the control and hemicastrated groups. Values obtained were 2.67 +/- 0.15 mU/mg, 3.77 +/- 0.4 mU/mg and 3.77 +/- 0.33 mU/mg, respectively (p less than 0.05). Using electrophoresis, 3 out of 18 rats had typical X bands, which is the classical picture of a normal mature testis. In contrast, the others showed faint X bands at the 2nd postoperative week, which subsequently faded thereafter. During testicular ischemia, the Leydig cells are more resistant than the Sertoli cells and the germinal cells.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Surgery, Anatomy, Tri-Service General Hospital National Defense Medical Center, Taipei, Taiwan, ROC.
authors.forename: E J
authors.initials: EJ
authors.lastname: Huang
authors.forename: H W
authors.initials: HW
authors.lastname: Liu
authors.forename: K L
authors.initials: KL
authors.lastname: Shen
authors.forename: C P
authors.initials: CP
authors.lastname: Ma
mesh_terms: D000818:Animals; D003456:Cryptorchidism / Q000601:surgery*; D007064:L-Iditol 2-Dehydrogenase / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008297:Male; D051381:Rats; D013737:Testis / Q000201:enzymology / Q000473:pathology / Q000601:surgery*; D013739:Testosterone / Q000097:blood
chemical_list: D013739:Testosterone; D007064:L-Iditol 2-Dehydrogenase; D007770:L-Lactate Dehydrogenase