Potentiation of ethyl methanesulfonate-induced germ cell mutagenesis and depression of glutathione in male reproductive tissues by 1,2-dibromoethane.
EDB significantly depressed GSH in caput and cauda epididymis, but not in testis, 2 hours following injection. This depression was dose-related. EDB enhanced EMS-induced dominant lethal mutations at mating weeks 2 and 3 (of 6). At mating week 2 the fetal death rate was increased two-fold, while at week 3, the fetal death rate had increased to nearly three-fold greater than the EMS-only controls. Enhancement of fetal death rate was confined to postimplantation loss. As with EMS alone, the EDB potentiation of EMS-induced mutations was limited to postmeiotic stages of spermatogenesis. EDB also enhanced alkylation of rat spermatozoa by labeled EMS. Depression of GSH in reproductive tissues is correlated with a potentiation of dominant lethal mutations, as well as an increase in the binding of EMS to sperm heads.
Teaf
C M
CM
Center for Biomedical & Toxicological Research and Hazardous Waste Management, Florida State University, Tallahassee 32306.
Bishop
J B
JB
Harbison
R D
RD
D000818:Animals; D004357:Drug Synergism; D010064:Embryo Implantation / Q000187:drug effects; D005020:Ethyl Methanesulfonate / Q000493:pharmacokinetics / Q000633:toxicity*; D015946:Ethylene Dibromide / Q000633:toxicity*; D005260:Female; D005313:Fetal Death; D005327:Fetal Resorption; D005799:Genes, Dominant; D005804:Genes, Lethal; D005837:Genitalia, Male / Q000187:drug effects / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D008297:Male; D016296:Mutagenesis; D009153:Mutagens; D011247:Pregnancy; D051381:Rats; D011916:Rats, Inbred F344; D013094:Spermatozoa / Q000378:metabolism
D009153:Mutagens; D015946:Ethylene Dibromide; D005020:Ethyl Methanesulfonate; D005978:Glutathione