title: An improved method for the detection of Dcm methylation in DNA molecules.
abstract: The intrinsic insensitivity of EcoRII recognition sites in RF DNAs of phage M13 and vector M13mp18 towards this restriction endonuclease can be overcome by adding site-specific oligodeoxyribonucleotide duplexes to the restriction sample. Since Dcm- DNA but not Dcm(+)-methylated DNA becomes susceptible under these conditions, this procedure constitutes an improvement of the Dcm methylation assay.
authors.affiliation: Institute of Virology, Humboldt-University, Berlin, G.D.R.
authors.forename: M
authors.initials: M
authors.lastname: Reuter
authors.forename: C D
authors.initials: CD
authors.lastname: Pein
authors.forename: V
authors.initials: V
authors.lastname: Butkus
authors.forename: D H
authors.initials: DH
authors.lastname: Krüger
mesh_terms: D001435:Bacteriophages / Q000235:genetics; D003596:Cytosine; D004247:DNA / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D008745:Methylation; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D003596:Cytosine; D004247:DNA