title: Tyrosine hydroxylase mRNA concentration in midbrain dopaminergic neurons is differentially regulated by reserpine.
abstract: Tyrosine hydroxylase (TH)-mRNA, assayed by in situ hybridization combined with TH immunocytochemistry, showed a selective increase in the ventral tegmental area (A-10) but not in the substantia nigra (A-9) midbrain dopaminergic (DAergic) neurons 3 days after reserpine treatment. TH-mRNA in locus ceruleus noradrenergic (A-4) neurons was increased by reserpine, as confirmed by RNA blot hybridization. These findings show that TH-mRNA is differentially regulated in midbrain DAergic neurons in response to reserpine.
authors.affiliation: Andrus Gerontology Center, University of Southern California, Los Angeles 90089-0191.
authors.forename: G M
authors.initials: GM
authors.lastname: Pasinetti
authors.forename: D G
authors.initials: DG
authors.lastname: Morgan
authors.forename: S A
authors.initials: SA
authors.lastname: Johnson
authors.forename: S L
authors.initials: SL
authors.lastname: Millar
authors.forename: C E
authors.initials: CE
authors.lastname: Finch
mesh_terms: D000818:Animals; D004210:Dissection / Q000379:methods; D004298:Dopamine / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D008636:Mesencephalon / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D009994:Osmolar Concentration; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D012110:Reserpine / Q000494:pharmacology*; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D012110:Reserpine; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine