Identification and cloning of a umu locus in Streptomyces coelicolor A3(2).
The umuDC operon of Escherichia coli is required for efficient mutagenesis by UV and many other DNA-damaging agents. E. coli umu mutants are defective in mutagenesis and slightly more sensitive to DNA-damaging agents. The existence of a umuDC analogue in Streptomyces coelicolor was suggested by data of our previous works. We cloned from Streptomyces coelicolor a fragment of DNA homologous to the E. coli umuDC region that is able to complement the E coli umuC122::Tn5 mutation. Therefore our data suggest that S. coelicolor contains a functional umu-like operon.
Misuraca
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F
Dipartimento di Biologia Cellulare e dello Sviluppo A. Monroy, University of Palermo, Italy.
Rampolla
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D
Grimaudo
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S
D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D009693:Nucleic Acid Hybridization; D009876:Operon; D012689:Sequence Homology, Nucleic Acid; D013302:Streptomyces / Q000235:genetics*; D014466:Ultraviolet Rays / Q000009:adverse effects
D004247:DNA