title: Variables affecting disc size in the lumbar spine of rabbits: anesthesia, paralysis, and disc injury.
abstract: Methods have been developed that permit repetitive radiographic measurement of the lumbar intervertebral disc space in a rostral-caudal direction (width) in the anesthetized laboratory rabbit. Using isolated control discs and injured discs in which narrowing has been induced for chronic and acute periods, the widths of the lumbar intervertebral disc spaces determined ratio-graphically correlate with widths determined histologically (p less than 0.000, r = 0.75). Both an increase (widening) and a decrease (narrowing) in disc width were observed using radiography after different experimental treatments. Anesthesia and lower-body paralysis (an experimentally induced inability to bear weight on and to perceive a pinch stimulus in hind limbs) caused widening of the discs: anesthesia causing a general widening throughout the lumbar spine and lower-body paralysis causing a specific widening low in the lumbar spine. Both disc injection and piercing the disc with needles to recover nucleus pulposus material caused narrowing of the discs. Acridine-orange injection induced a narrowing accompanied by osteophytosis. Experimentally induced narrowing at L4-5 (the result of injury to the disc) resulted in narrowing also at L2-3. These findings are consistent with the hypothesis that in vivo disc-width size in the young rabbit depends on both the quantity of nucleus pulposus material and the force-generating activities of the adjacent spinal muscles, and that disc injury at one level stimulates narrowing at other levels.
authors.affiliation: VA Medical Center, San Diego, California.
authors.forename: J H
authors.initials: JH
authors.lastname: Neufeld
authors.forename: T
authors.initials: T
authors.lastname: Machado
authors.forename: L
authors.initials: L
authors.lastname: Margolin
mesh_terms: D000075:Acepromazine / Q000494:pharmacology; D000775:Anesthesia, Spinal; D000818:Animals; D006221:Halothane / Q000494:pharmacology; D007403:Intervertebral Disc / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology*; D007649:Ketamine / Q000494:pharmacology; D008159:Lumbar Vertebrae / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology*; D010243:Paralysis / Q000473:pathology; D011817:Rabbits; D011859:Radiography; D013122:Spinal Diseases / Q000473:pathology
chemical_list: D000075:Acepromazine; D007649:Ketamine; D006221:Halothane