title: Synthetic peptides including acidic clusters as substrates of yeast casein kinase-2.
abstract: The synthesis is reported of a series of glutamyl peptide analogs of the model substrate H-Ser-Glu-Glu-Glu-Glu-Glu-OH of casein kinase-2 (CK-2). A convenient HPLC method for the separation of slightly different acidic peptides is also reported. The site specificity of yeast casein kinase-2 (Y-CK2) is examined with the aid of synthesized peptide substrates.
authors.affiliation: Biopolymer Research Centre, C.N.R., Department of Organic Chemistry, University of Padova, Italy.
authors.forename: O
authors.initials: O
authors.lastname: Marin
authors.forename: A
authors.initials: A
authors.lastname: Calderan
authors.forename: P
authors.initials: P
authors.lastname: Ruzza
authors.forename: G
authors.initials: G
authors.lastname: Borin
authors.forename: F
authors.initials: F
authors.lastname: Meggio
authors.forename: N
authors.initials: N
authors.lastname: Grankowski
authors.forename: F
authors.initials: F
authors.lastname: Marchiori
mesh_terms: D000595:Amino Acid Sequence; D047388:Casein Kinases; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005971:Glutamates; D018698:Glutamic Acid; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000302:isolation & purification / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D005971:Glutamates; D007202:Indicators and Reagents; D009842:Oligopeptides; D018698:Glutamic Acid; D011494:Protein Kinases; D047388:Casein Kinases