Supramolecular organization of double-stranded DNA molecules in the columnar hexagonal liquid crystalline phase. An electron microscopic analysis using freeze-fracture methods.
I present an electron microscopical analysis of the columnar hexagonal liquid crystalline phase of DNA. Freeze-fracture methods reveal that this phase is a lamellar structure, each layer (30 to 40 A thick) composed of DNA molecules aligned in parallel. Numerous defects can be seen in the structure, and their nature is determined. I show that they are mainly screw dislocations of both handedness. By this method it is possible to follow individual double-stranded DNA molecules in this highly packed structure. I show, moreover, that there is a local twist between DNA molecules along the screw dislocation lines and that this twist can be either right-handed or left-handed. The interest of such ultrastructural analysis is discussed in relation to the understanding of chromatin structure.
Livolant
F
F
Centre de Biologie Cellulaire (CNRS), Ivry-sur-Seine, France.
D000818:Animals; D002417:Cattle; D002843:Chromatin / Q000648:ultrastructure; D004247:DNA / Q000648:ultrastructure*; D005614:Freeze Fracturing / Q000379:methods; D008836:Micrococcal Nuclease; D008854:Microscopy, Electron / Q000379:methods; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D013950:Thymus Gland
D002843:Chromatin; D004247:DNA; D008836:Micrococcal Nuclease