HLA-DQ RFLP variants of five HLA-DQw2-bearing major histocompatibility complex extended haplotypes.
We have analyzed genomic DNA in a large number of independent examples of five HLA-DQw2-bearing extended haplotypes for their associated subtypes by restriction fragment length polymorphism (RFLP) using DRB, DQA, and DQB probes after Taq I and Pst I digestion and Southern blotting. In addition to three previously described HLA-DQw2 subtypes, DQw2a, DQw2b, and DQw2c, we observed a fourth subtype, HLA-DQw2d, characterized by 5.8 kilobase (kb) DRB/Taq I, 2.4, 2.3, and 1.8 kb DQB/Taq I, and 8.0 and 2.3 kb DQA/Pst I fragments. All 22 independent examples of the extended haplotype [HLA-B8, SC01, DR3] carried DQw2a and all 11 independent examples of [HLA-B18, F1C30, DR3] carried DQw2b. In addition, all independent examples (21 and 4, respectively) of two DR7-carrying extended haplotypes, [HLA-B44, FC31, DR7] and [HLA-Bw47, FC91,0,DR7], carried DQw2c and all independent examples of [HLA-Bw57, SC61, DR7] carried DQw2d. Our results show that the DNA in the DR/DQ region of extended haplotypes is relatively fixed and that different DQw2 subtypes characterize different DQw2-bearing extended haplotypes.
Yunis
J J
JJ
Division of Immunogenetics, Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
Wescott
M
M
Lechin
S J
SJ
Alosco
S M
SM
Awdeh
Z
Z
Alper
C A
CA
Yunis
E J
EJ
D015139:Blotting, Southern; D015342:DNA Probes; D006683:HLA-DQ Antigens / Q000235:genetics*; D015803:HLA-DR3 Antigen / Q000235:genetics; D015809:HLA-DR7 Antigen / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D008285:Major Histocompatibility Complex / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
D015342:DNA Probes; D006683:HLA-DQ Antigens; D015803:HLA-DR3 Antigen; D015809:HLA-DR7 Antigen