Cloning of genomic and complementary DNA encoding insect pheromone binding proteins: evidence for microdiversity.
Genomic DNA from the silk moth Antheraea pernyi bearing the gene of a pheromone binding protein has been isolated from a partial genomic library using specific cDNA probes. The DNA spans 3.5 kilobases, contains three exons and two intervening sequences that interrupt the protein coding region of the gene. A DNA fragment of a second gene was isolated and the complete primary structure of a corresponding cDNA clone was unravelled. The expression of two different genes, giving rise to different pheromone binding proteins, implies a more specific function of these proteins than was hitherto assumed.
Krieger
J
J
University Stuttgart-Hohenheim, Institute of Zoophysiology, F.R.G.
Raming
K
K
Breer
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D015698:Genomic Library; D007301:Insect Hormones / Q000235:genetics*; D019476:Insect Proteins; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D002352:Carrier Proteins; D007301:Insect Hormones; D019476:Insect Proteins; D036341:Intercellular Signaling Peptides and Proteins; C067715:APR-1 pheromone-binding protein, Antheraea pernyi; D004247:DNA