title: Patterns of protein synthesis in various cells after extreme heat shock.
abstract: The analysis of proteins synthesized in rat thymocytes and mouse teratocarcinoma PCC-4 Aza 1 and myeloma Sp2/0 cells after 1 h of treatment at 42 or 44 degrees C was carried out. Shock at 42 degrees C reduced the total synthetic rate of proteins in all three cell lines and induced "classical" heat-shock protein with a mass of 70 kDa (hsp 70). Heat shock at 44 degrees C resulted in almost complete inhibition of protein synthesis; only a small amount of hsp 70 was synthesized. Meanwhile a new 48-kDa polypeptide (pI = 7.5) was found in the cells exposed to severe heat shock. This protein was compared by peptide mapping with other known polypeptides of the same size: heat-shock protein from chicken embryo cells and mitogen-stimulated polypeptide from human lymphoid cells. The peptide maps were not identical. It was also shown that after a shock at 44 degrees C teratocarcinoma cells were able to accumulate anomalous amounts of hsp 70 despite hsp 70 synthesis inhibition. The data show that reaction of various cells to extreme heat shock depends heavily on cell type.
authors.affiliation: Institute of Cytology, Academy of Sciences USSR, Leningrad.
authors.forename: B A
authors.initials: BA
authors.lastname: Margulis
authors.forename: B D
authors.initials: BD
authors.lastname: Zhivotovski
authors.forename: T V
authors.initials: TV
authors.lastname: Pospelova
authors.forename: L V
authors.initials: LV
authors.lastname: Smagina
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D051379:Mice; D008970:Molecular Weight; D009101:Multiple Myeloma / Q000378:metabolism; D051381:Rats; D013601:T-Lymphocytes / Q000378:metabolism; D013724:Teratoma / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D006360:Heat-Shock Proteins