Growth inhibition of human keratinocytes by antisense c-myc oligomer is not coupled to induction of differentiation.
To study the relationship between cell growth and differentiation in human keratinocytes, we examined the effect of the antisense oligomer of c-myc mRNA. This oligomer is stable in culture medium. A 24 h incubation of cells with 5 microM antisense c-myc oligomer resulted in a 48.2% decrease in c-myc protein and inhibited cell growth by 80.7% compared to the sense c-myc oligomer. In contrast, antisense c-myc oligomer had no effect on differentiation when the population of involucrin-positive cells and cornified envelope formation were used as differentiation markers. These results show that antisense c-myc oligomer inhibits cell growth but does not induce differentiation in normal human keratinocytes. Therefore, cell growth and differentiation are not necessarily coupled in these cells.
Hashiro
M
M
Department of Dermatology, Osaka University School of Medicine, Japan.
Matsumoto
K
K
Okumura
H
H
Hashimoto
K
K
Yoshikawa
K
K
D001483:Base Sequence; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D002455:Cell Division; D002478:Cells, Cultured; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects; D008969:Molecular Sequence Data; D016372:RNA, Antisense / Q000235:genetics*; D012333:RNA, Messenger / Q000737:chemistry
D016372:RNA, Antisense; D012333:RNA, Messenger; D001973:Bromodeoxyuridine