Inhibitory effect of the somatostatin analog octreotide on rat pituitary tumor cell (GH3) proliferation in vitro.
The effects of somatostatin-14 (SS-14) and the somatostatin-analog octreotide (SMS 201-995, Sandostatin) on proliferation of GH3 pituitary tumor cells were investigated in vitro. SMS 201-995 exerted a significant, but transient, inhibition on GH3 cell growth which reached a maximum at 24 h and was no longer detectable at 48 h. The concentration that evoked the strongest inhibitory effect was 10 nM SMS 201-995, while lower and higher doses resulted in a less pronounced effect. The inhibitory effect SMS 201-995 exerted on cell proliferation was associated with a dose- and time-related reduction in both c-myc and c-fos mRNA levels. SS-14 had no noteworthy influence on either cell proliferation or c-myc and c-fos protooncogene expression. These data demonstrate that SS-analogs transiently inhibit pituitary tumor cell proliferation in vitro.
Pelicci
G
G
Istituto di Clinica Medica I, Università di Perugia, Italy.
Pagliacci
M C
MC
Lanfrancone
L
L
Pelicci
P G
PG
Grignani
F
F
Nicoletti
I
I
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003124:Colorimetry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D016259:Genes, myc / Q000187:drug effects; D015282:Octreotide / Q000494:pharmacology*; D010911:Pituitary Neoplasms / Q000235:genetics / Q000473:pathology*; D011519:Proto-Oncogenes / Q000187:drug effects; D051381:Rats; D013004:Somatostatin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D013004:Somatostatin; D015282:Octreotide