Phosphatidyl-Tris rather than N-acylphosphatidylserine is synthesized by Rhodopseudomonas sphaeroides grown in Tris-containing media.
We have synthesized 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho(N-oleoyl)serine (N-acyl-PS) and 1,2-dioleoyl-sn-glycero-3-phospho-Tris (phosphatidyl-Tris) and have characterized both phospholipids by their chemical and chromatographic properties, as well as by their IR, 13C NMR, and 1H NMR spectra. Comparison of these data with those reported for a phospholipid isolated from Rhodopseudomonas sphaeroides grown in Tris-supplemented media [Donohue et al. (1982) Biochemistry 21, 2765-2773] indicates that R. sphaeroides synthesizes phosphatidyl-Tris rather than N-acyl-PS.
Schmid
P C
PC
Hormel Institute, University of Minnesota, Austin 55912.
Kumar
V V
VV
Weis
B K
BK
Schmid
H H
HH
D000215:Acylation; D003470:Culture Media; D020404:Glycerophospholipids; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008745:Methylation; D010712:Phosphatidic Acids / Q000378:metabolism*; D010718:Phosphatidylserines / Q000378:metabolism*; D012242:Rhodobacter sphaeroides / Q000378:metabolism*; D014325:Tromethamine / Q000378:metabolism* / Q000031:analogs & derivatives
D003470:Culture Media; D020404:Glycerophospholipids; D010712:Phosphatidic Acids; D010718:Phosphatidylserines; D014325:Tromethamine; C067348:1,2-dioleoyl-sn-glycero-3-phospho-tris; C050294:1-palmitoyl-2-oleoylglycero-3-phosphoserine