title: Influence of chronic oral intake of cannabis extract on oxidative and hydrolytic metabolism of xenobiotics in rat.
abstract: Dietary intake of petroleum ether extract of cannabis leaves by rats in doses of 158, 250 and 500 mg/kg in the first, second and third week, respectively, caused selective induction of hepatic microsomal carboxylesterases/amidases without affecting the renal hydrolytic activity. Acetanilide N-deacetylase, p-nitrophenylacetate (NPA) esterase and acetylsalicylic acid (ASA) esterase I and II (active at pH 5.5 and 7.4) were stimulated 125, 64, 82 and 60%, respectively, whereas the activities of procaine esterase and acetylaminofluorene (AAF) N-deacetylase remained unaltered. The hydrolysis of acetylcholine was also unchanged. Upon withdrawal of treatment microsomal hydrolytic activity receded to basal levels within 7 days. Curiously though, the two-fold induction of thiacetazone N-deacetylase (118%), a cytosolic hydrolase, remained largely undiminished (62%). An appraisal of the hepatic cytochrome P450 mediated oxidative metabolism revealed approximately three-fold induction of aromatic hydrocarbon hydroxylase (AHH) metabolizing benzo(a)pyrene whereas the N-demethylation of aminopyrene was unaffected. These activities were restored to normal when resin administration was discontinued.
authors.affiliation: Department of Biochemistry, King George's Medical College, Lucknow, India.
authors.forename: P
authors.initials: P
authors.lastname: Khanna
authors.forename: M B
authors.initials: MB
authors.lastname: Gupta
authors.forename: G P
authors.initials: GP
authors.lastname: Gupta
authors.forename: G G
authors.initials: GG
authors.lastname: Sanwal
authors.forename: B
authors.initials: B
authors.lastname: Ali
mesh_terms: D000581:Amidohydrolases / Q000096:biosynthesis*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002186:Cannabinoids / Q000008:administration & dosage / Q000494:pharmacology*; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis*; D002802:Cholinesterases / Q000096:biosynthesis*; D004032:Diet; D004790:Enzyme Induction / Q000187:drug effects; D007668:Kidney / Q000187:drug effects; D008861:Microsomes / Q000187:drug effects; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D015262:Xenobiotics / Q000378:metabolism*
chemical_list: D002186:Cannabinoids; D015262:Xenobiotics; D002265:Carboxylic Ester Hydrolases; D002802:Cholinesterases; D000581:Amidohydrolases