Localization and quantitation of tritiated compounds in tissue sections with a gaseous detector of beta particles: comparison with film autoradiography.
Quantitative analysis of tritium polymer standards and of brain sections labeled with tritiated vasopressin was carried out by using a gaseous detector of beta particles designed for this purpose. The gaseous detector showed major advantages compared with film autoradiography: the linearity and the large dynamic range of intensity measurements as well as the short time needed for data acquisition.
Tribollet
E
E
Department of Physiology, University Medical Centre, Geneva, Switzerland.
Dreifuss
J J
JJ
Charpak
G
G
Dominik
W
W
Zaganidis
N
N
D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism; D001345:Autoradiography / Q000379:methods; D001921:Brain / Q000166:cytology*; D011865:Radioisotope Dilution Technique; D051381:Rats; D011945:Receptors, Angiotensin / Q000032:analysis*; D017483:Receptors, Vasopressin; D014316:Tritium
D011945:Receptors, Angiotensin; D017483:Receptors, Vasopressin; D014316:Tritium; D001127:Arginine Vasopressin