title: Beneficial effect of liposome-encapsulated muramyl tripeptide in experimental septicemia in a porcine model.
abstract: In a porcine model of pneumococcal septicemia, animals were pretreated with 1 mg of liposome-encapsulated muramyl tripeptide phosphatidylethanolamine (MTP-PE) or with liposomes alone. After 24 h each animal received an injection of either 10(9) or 10(10) pneumococcal serotype 6B cells. MTP-PE pretreatment resulted in less pronounced leukocytopenia, with a nadir of 6,700 (versus 4,100) leukocytes per mm3 after injection of 10(9) bacteria and a nadir of 4,400 (versus 3,800) leukocytes per mm3 after injection of 10(10) bacteria. At the same time bacterial clearance was substantially improved by MTP-PE pretreatment. Finally, pretreatment with MTP-PE dramatically reduced mortality; the average death rates for both series of animals used were 55% for liposome-pretreated animals and 3% for animals pretreated with MTP-PE-containing liposomes. These results in a preclinical model suggest that treatment with MTP-PE-containing liposomes might be beneficial in controlling septicemia in patients at risk.
authors.affiliation: Department of Surgery, University of Munich, Federal Republic of Germany.
authors.forename: J R
authors.initials: JR
authors.lastname: Izbicki
authors.forename: C
authors.initials: C
authors.lastname: Raedler
authors.forename: A
authors.initials: A
authors.lastname: Anke
authors.forename: P
authors.initials: P
authors.lastname: Brunner
authors.forename: M
authors.initials: M
authors.lastname: Siebeck
authors.forename: E
authors.initials: E
authors.lastname: Leinisch
authors.forename: R
authors.initials: R
authors.lastname: Lüttiken
authors.forename: G
authors.initials: G
authors.lastname: Ruckdeschel
authors.forename: D K
authors.initials: DK
authors.lastname: Wilker
authors.forename: L
authors.initials: L
authors.lastname: Schweiberer
mesh_terms: D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D007958:Leukocyte Count; D008081:Liposomes / Q000008:administration & dosage; D009000:Monocytes / Q000276:immunology; D011008:Pneumococcal Infections / Q000188:drug therapy; D018805:Sepsis / Q000188:drug therapy* / Q000401:mortality; D013534:Survival; D013552:Swine; D013556:Swine, Miniature
chemical_list: D008081:Liposomes; D000119:Acetylmuramyl-Alanyl-Isoglutamine; C035104:muramylNAc-Ala-isoGln-Lys-tripeptide