Atrial natriuretic factor activates membrane-bound guanylate cyclase of chief cells.
The present study examined the effect of atrial natriuretic factor (ANF) on cGMP generation by dispersed chief cells from guinea pig stomach. ANF caused a rapid dose-dependent increase in cGMP, a 7-fold increase in cGMP caused by 1 microM ANF, with or without 3-isobutyl-1-methylxanthine present. Methylene blue reduced cGMP in response to nitroprusside but not ANF. Guanylate cyclase activity of a chief cell membrane fraction doubled in response to ANF, but was not affected by nitroprusside. ANF had no effect on guanylate cyclase activity of the soluble fraction of lysed chief cells. Dose-response curves for whole cell cGMP production and membrane guanylate cyclase activity in response to ANF were closely related. These data indicate that ANF increases chief cell cGMP production by activating particulate guanylate cyclase, providing functional evidence that chief cells possess surface membrane receptors for ANF.
Cherner
J A
JA
Division of Gastroenterology, DVA Medical Center, Nashville, Tennessee.
Singh
G
G
Naik
L
L
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism / Q000494:pharmacology*; D002462:Cell Membrane / Q000201:enzymology; D006152:Cyclic GMP / Q000096:biosynthesis; D004789:Enzyme Activation; D005753:Gastric Mucosa / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D006162:Guanylate Cyclase / Q000378:metabolism*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D009599:Nitroprusside / Q000494:pharmacology; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000032:analysis
D011956:Receptors, Cell Surface; D009599:Nitroprusside; D009320:Atrial Natriuretic Factor; D006162:Guanylate Cyclase; D017461:Receptors, Atrial Natriuretic Factor; D006152:Cyclic GMP