title: Pancreatobiliary diversion enhances experimental pancreatic carcinogenesis.
abstract: Since compensatory hyperplasia promotes experimental carcinogenesis in the gut, we tested the ability of two surgical models of pancreatic growth to promote pancreatic carcinogenesis. Male Wistar rats (n = 60) weighing 250-300 g underwent pancreatobiliary diversion (PBD), 90% small bowel resection (PSBR) or triple transection and reanastomosis of the small intestine (controls). Postoperatively, each group received azaserine (20 mg kg-1 wk-1 i.p.) for 6 weeks. Surviving rats were killed at 6 months, pancreatic wet weight was measured and histological sections were examined for atypical acinar cell foci (AACF), the putative precursor of carcinoma. Median relative pancreatic weight (mg pancreas/g body weight) was 2.20 for controls (n = 18), 4.08 for PSBR (n = 11) (P less than 0.001) and 6.86 for PBD (n = 16) (P less than 0.001). PSBR did not affect the development of acidophilic AACF, but PBD produced an enormous increase in their number per cm3 (median 96 vs. 0; P less than 0.001) and a 7-fold increase in their volume (P less than 0.001). Both operations cause pancreatic growth, but only PBD promotes carcinogenesis, possibly because of its unique hormonal effect.
authors.affiliation: Department of Surgery, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
authors.forename: I D
authors.initials: ID
authors.lastname: Stewart
authors.forename: B
authors.initials: B
authors.lastname: Flaks
authors.forename: P
authors.initials: P
authors.lastname: Watanapa
authors.forename: P W
authors.initials: PW
authors.lastname: Davies
authors.forename: R C
authors.initials: RC
authors.lastname: Williamson
mesh_terms: D000818:Animals; D015904:Biliopancreatic Diversion / Q000009:adverse effects*; D001835:Body Weight / Q000502:physiology; D007422:Intestines / Q000601:surgery; D008297:Male; D009374:Neoplasms, Experimental / Q000209:etiology* / Q000473:pathology; D009929:Organ Size / Q000502:physiology; D010179:Pancreas / Q000033:anatomy & histology / Q000254:growth & development / Q000601:surgery; D010190:Pancreatic Neoplasms / Q000209:etiology* / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains