title: Effects of cytochalasin D and methylamine on intracellular growth of Legionella pneumophila in amoebae and human monocyte-like cells.
abstract: A cloned and axenically cultured strain of Hartmannella vermiformis was used as a model to study intracellular multiplication of Legionella pneumophila in amoebae. The growth of L. pneumophilia in both H. vermiformis and a human monocyte-like cell line (U937) was investigated with cytoskeletal and metabolic inhibitors. L. pneumophila replicated only intracellularly in these cellular models, and electron microscopy showed ultrastructural similarities in the initial phase of multiplication. Treatment of amoebae with an inhibitor of microfilament-dependent phagocytosis (cytochalasin D, 0.5 or 1.0 micrograms/ml) did not inhibit intracellular growth of L. pneumophila; however, intracellular multiplication was inhibited by treatment of U937 monocytes with the same concentrations of cytochalasin D. Methylamine (10 to 100 mM), an inhibitor of adsorptive pinocytosis, inhibited the replication of L. pneumophila in amoebae in a dose-dependent manner. All doses of methylamine tested (10 to 50 mM) inhibited growth of L. pneumophila in U937 monocytes. Cytochalasin D and methylamine had no effect on the multiplication of L. pneumophila in culture medium or on the viability of amoebae or U937 monocytes. Intracellular replication of L. pneumophila in H. vermiformis may be accomplished by a cytochalasin D-independent mechanism, such as adsorptive pinocytosis. In contrast, both cytochalasin D- and methylamine-sensitive mechanisms may be essential for the intracellular multiplication of L. pneumophila in U937 monocytes.
authors.affiliation: Department of Medical Microbiology, College of Veterinary Medicine, University of Georgia, Athens 30602.
authors.forename: C H
authors.initials: CH
authors.lastname: King
authors.forename: B S
authors.initials: BS
authors.lastname: Fields
authors.forename: E B
authors.initials: EB
authors.lastname: Shotts
authors.forename: E H
authors.initials: EH
authors.lastname: White
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002460:Cell Line; D015169:Colony Count, Microbial; D015638:Cytochalasin D / Q000494:pharmacology*; D006249:Hartmannella / Q000187:drug effects / Q000382:microbiology* / Q000648:ultrastructure; D006801:Humans; D007875:Legionella / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D008744:Methylamines / Q000494:pharmacology*; D009000:Monocytes / Q000187:drug effects / Q000382:microbiology* / Q000648:ultrastructure
chemical_list: D008744:Methylamines; D015638:Cytochalasin D; C027451:methylamine