Synthesis of gamma-[4-(benzoylphenyl)methylamido]-7-methylguanosine 5'-triphosphate, a photoaffinity-label for cap-binding proteins.
An easy and rapid method for the synthesis of the photoreactive cap analogue gamma-[4-(benzoylphenyl)methylamido]-7-methylguanosine-5'-triphosp hate (BP-m7GTP) has been developed. Synthesis of [gamma-32P]BP-m7GTP was carried out as a two-step procedure. The first step included the phosphorylation of GDP with [32P]orthophosphate to [gamma-32P]GTP and its methylation to [gamma-32P]m7GTP. After purification, [gamma-32P]m7GTP was condensed with 4-aminomethyl-benzophenone-HCl to [gamma-32P]BP-m7GTP. Using this probe, specific labeling of nuclear cap binding proteins is demonstrated.
Thalmann
E
E
Institute for Biochemistry, University of Vienna, Austria.
Blaas
D
D
D000345:Affinity Labels / Q000138:chemical synthesis*; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D003432:Cross-Linking Reagents; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism; D007202:Indicators and Reagents; D008970:Molecular Weight; D010766:Phosphorylation; D039381:RNA Cap-Binding Proteins; D012315:RNA Caps / Q000378:metabolism
D000345:Affinity Labels; D002352:Carrier Proteins; D003432:Cross-Linking Reagents; D007202:Indicators and Reagents; D039381:RNA Cap-Binding Proteins; D012315:RNA Caps; D006153:Guanosine Diphosphate; C033802:gamma-(4-(benzoylphenyl)methylamido)-7-methylguanosine-5'-triphosphate; D006160:Guanosine Triphosphate