Mapping of the Hox-3.1 and Myc-1.2 genes on chromosome 15 of the mouse by restriction fragment length variations.
Restriction endonuclease fragment length variations (RFLV) were detected by use of the cDNA probe Hox-3.1 for the homeo box-3.1 gene and also the c-myc oncogene probe for exon 2. RFLV of Hox-3.1 were found in HindIII restriction patterns, and RFLV of the Myc-1.2 gene in EcoRV patterns. From the RFLV, the Hox-3.1 and Myc-1.2 genes were mapped on chromosome 15. Three-point cross test data showed that the frequency of recombination is 26.4% between Myc-1.2 and Gpt-1, 30.2% between Gpt-1 and Gdc-1, and 9.4% between Gdc-1 and Hox-3.1. The following order of these genes is proposed, Myc-1.2--Gpt-1--Gdc-1--Hox-3.1. All laboratory strains carry the Hox-3.1a and Myc-1.2a alleles. Among strains of wild origin, domesticus strains carry only the Hox-3.1a and Myc-1.2a alleles, as do the laboratory strains. One strain of brevirostris carries the Hox-3.1a and Myc-1.2b alleles. Other wild subspecies from Europe and Asia, M. m. musculus, M. m. castaneus, M. m. molossinus, Chinese mice of wild origin, and M. m. yamashinai carry the Hox-3.1b and Myc-1.2b alleles.
Watanabe
T
T
Department of Biochemistry, Aichi Prefecture Colony, Japan.
Ohno
K
K
Shimizu
A
A
Sakai
Y
Y
Takahashi
M
M
Takahashi
N
N
D000483:Alleles; D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D005260:Female; D005796:Genes; D005801:Genes, Homeobox; D008040:Genetic Linkage; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009857:Oncogenes; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic