Use of nuclear DNA restriction fragment length polymorphisms to analyze the diversity of the Aspergillus flavus group: A. flavus, A. parasiticus, and A. nomius.
Recombinant DNA clones carrying high-copy or low-copy sequences from Aspergillus nidulans and Neurospora crassa were used to identify restriction fragment length polymorphisms (RFLPs) diagnostic for members of the A. flavus group: A. flavus, A. parasiticus, and A. nomius. These fungi were resolved into three distinct categories when they were grouped according to RFLP patterns. Subgroups within these categories were also evident. This limited RFLP analysis of nuclear DNA of members of the A. flavus group did not identify any RFLPs that differentiate these isolates on the basis of toxin production, but limited correlation with geographic location was observed.
Moody
S F
SF
Research School of Biological Sciences, Australian National University, Canberra, Australian Capital Territory.
Tyler
B M
BM
D001230:Aspergillus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D001231:Aspergillus flavus / Q000145:classification / Q000235:genetics; D015342:DNA Probes; D004271:DNA, Fungal / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D005784:Gene Amplification; D005819:Genetic Markers; D014644:Genetic Variation; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
D015342:DNA Probes; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D005819:Genetic Markers