Tumor necrosis factor-alpha inhibits the competence signal delivered by HIV to normal B cells.
Polyclonal B cell activation is commonly observed in HIV-infected patients. The coordinate delivery of a number of signals is required for B cell response. This work was designed to better define the role of HIV in the first steps of normal human B cells activation. We show that the infectious virus or recombinant envelope proteins can render B cells responsive to the growth-promoting effect of several T cell-derived IL, IL-2, IL-4, and low m.w. (12-kDa) BCGF. HIV acts in the absence of monocytes and on different populations of B cells. The competence signal can be provided by recombinant gp160 envelope protein. CD4 molecule is not involved in the interaction of HIV with B cells. In addition, we demonstrate that tumor necrosis factor alpha has no promoting activity when B cells are preactivated by HIV and it can suppress the response of HIV-preactivated B cells to IL-2, IL-4, and 12-kDa BCGF. Thus, the HIV envelope can deliver an early signal to normal B cells and modulate B cell response to physiologic signals. The possible relevance of this phenomenon to the immune defect observed in HIV patients is discussed.
Delfraissy
J F
JF
INSERM, U131, Hôpital Antoine Béclère, Clamart, France.
Wallon
C
C
Boue
F
F
Barresinoussi
F
F
Galanaud
P
P
D001402:B-Lymphocytes / Q000276:immunology* / Q000382:microbiology*; D015686:Gene Products, env / Q000276:immunology; D006678:HIV / Q000276:immunology*; D019302:HIV Envelope Protein gp160; D006801:Humans; D007378:Interleukins / Q000502:physiology; D008213:Lymphocyte Activation / Q000502:physiology*; D011498:Protein Precursors / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*
D015686:Gene Products, env; D019302:HIV Envelope Protein gp160; D007378:Interleukins; D011498:Protein Precursors; D014409:Tumor Necrosis Factor-alpha