Outcome following femur fracture and subsequent cecal ligation and puncture in endotoxin-sensitive (C3H/HeN) and endotoxin-resistant (C3H/HeJ) mice.
This study examined the effect of sepsis following trauma in a reproducible model of sepsis--cecal ligation and puncture (CLP)--in endotoxin-sensitive (C3H/HeN) and endotoxin-resistant (CeH/HeJ) mice. Studies used CLP with a 25-gauge needle at different time intervals following injury, as induced by femur fracture (FF), to determine the effects of sublethal sepsis on survival after trauma. There was a 3% mortality for FF alone in both groups. Mortality in C3H/HeJ mice was not significantly increased over FF alone except when CLP followed FF by 3 days (45%, P less than 0.02, Chi-square). In contrast, C3H/HeN mice had significantly increased mortality rates (75 to 90%, P less than 0.001) versus FF alone at all intervals between FF and CLP. Mortality for FF plus CLP was significantly greater for C3H/HeN compared to C3H/HeJ (P less than 0.001) for all time intervals between FF and CLP. In conclusion, animals exposed to a septic episode following FF had significantly greater mortality than FF animals without a septic challenge. Endotoxin-sensitive mice had significantly higher mortality after CLP and significantly increased mortality when CLP followed FF (regardless of timing) compared to endotoxin-resistant mice.
Baker
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Department of Surgery, Yale University School of Medicine, New Haven, Connecticut 06510.
Niven-Fairchild
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Caragnano
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Kupper
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TS
D000818:Animals; D002432:Cecum / Q000502:physiology* / Q000601:surgery; D004195:Disease Models, Animal; D004731:Endotoxins / Q000633:toxicity*; D005264:Femoral Fractures / Q000150:complications*; D007113:Immunity, Innate; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D018805:Sepsis / Q000209:etiology / Q000503:physiopathology*; D013045:Species Specificity
D004731:Endotoxins