Expression of the cholecystokinin gene in a human (small-cell) lung carcinoma cell-line.
Expression of the cholecystokinin (CCK), gastrin and enkephalin A genes were studied by Northern blot analysis and a library of sequence-specific radioimmunoassays in human cell lines. The human small-cell lung carcinoma line (SCLC) U-1690 expressed moderate levels of CCK mRNA as compared to the human neuroepithelioma cell line SK-N-MC. Neither gastrin nor (pro)enkephalin A mRNAs were detectable in the U-1690 cell line. In contrast, the SCLC-line H-69 expressed Enk A but no CCK mRNA. The radioimmunoassays showed that the CCK mRNA transcript in the SCLC line U-1690 also is translated, and that preproCCK is processed into bioactive, carboxyamidated CCK peptides. Thus, the human small cell carcinoma cell line U-1690 is a useful model for studies of cell-specific CCK gene expression.
Geijer
T
T
Department of Clinical Biochemistry (KK 6321), State University Hospital (Rigshospitalet), Copenhagen, Denmark.
Folkesson
R
R
Rehfeld
J F
JF
Monstein
H J
HJ
D000818:Animals; D018288:Carcinoma, Small Cell / Q000235:genetics*; D002766:Cholecystokinin / Q000096:biosynthesis* / Q000235:genetics*; D004745:Enkephalins / Q000235:genetics; D005755:Gastrins / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D011061:Poly A / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D014407:Tumor Cells, Cultured
D004745:Enkephalins; D005755:Gastrins; D012333:RNA, Messenger; D011061:Poly A; D002766:Cholecystokinin