Is interindividual variation of cellular radiosensitivity real or artifactual?
A recently developed dose-survival assay using human G0 T lymphocytes from peripheral blood was employed to assess possible interindividual variation of cellular radiosensitivity by comparing variability between a single test for different individuals and repeated tests for a single donor. The surviving fraction at each X-ray dose level fluctuated similarly between the two groups, and the X-ray dose required to kill 90% of the cells (D10) was 3.59 +/- 0.18 Gy (mean +/- SD) for 31 different individuals and 3.66 +/- 0.21 Gy for 28 repeated tests of one individual. Analysis of variance to compare the two sets of data showed that variation in the D10 value was not significantly greater in the former group. Analysis of D50 and D90 showed similar results. These results support the hypothesis that interindividual variation in cellular radiosensitivity is quite small, if it exists at all, as far as can be determined by the loss of colony-forming ability of irradiated G0 lymphocytes.
Nakamura
N
N
Department of Radiobiology, Radiation Effects Research Foundation, Hiroshima, Japan.
Sposto
R
R
Kushiro
J
J
Akiyama
M
M
D002470:Cell Survival / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D014644:Genetic Variation / Q000235:genetics*; D006801:Humans; D011836:Radiation Tolerance / Q000235:genetics*; D013601:T-Lymphocytes / Q000528:radiation effects*