Double-label analyses of somatostatin's coexistence with enkephalin and gamma-aminobutyric acid in amacrine cells of the chicken retina.
Double-label analyses were performed to investigate somatostatin's coexistence with either enkephalin or gamma-aminobutyric acid (GABA) in amacrine cells of the chicken retina. Double-label immunocytochemistry revealed that although some amacrine cells labelled only for somatostatin or enkephalin, approx. 81% and 85% of somatostatin-immunopositive cells in the center and periphery of the retina, respectively, were also enkephalin-immunoreactive. Somatostatin-immunocytochemistry combined with autoradiography of high-affinity [3H]GABA uptake revealed that approx. 18% of somatostatin-immunoreactive amacrine cells exhibit high-affinity uptake of [3H]GABA.
Li
H B
HB
Department of Histology and Embryology, Zhongshan Medical College, Guangzhou People's Republic of China.
Watt
C B
CB
Lam
D M
DM
D000818:Animals; D000911:Antibodies, Monoclonal; D002645:Chickens; D004745:Enkephalins / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D012160:Retina / Q000166:cytology*; D012165:Retinal Ganglion Cells / Q000166:cytology; D013004:Somatostatin / Q000032:analysis*; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D000911:Antibodies, Monoclonal; D004745:Enkephalins; D013004:Somatostatin; D005680:gamma-Aminobutyric Acid