title: Production of heparanase by normal and neoplastic murine B-lymphocytes.
abstract: The production of heparanase, an endoglycosidase capable of degrading heparan sulfate from the subendothelial extracellular matrix (ECM), was investigated in various murine B-lymphoid tumors representing distinct maturation stages of the B-cell lineage. We found that heparanase is produced and released by 3 out of 4 pre-B lymphomas and by 4 B lymphomas examined. In contrast, 5 plasmacytomas and resting normal B lymphocytes, expressed little, if any, heparanase activity. Treatment with LPS resulted in high expression of the enzyme by normal B-lymphocytes, but there was no effect on the constitutive production of heparanase by myeloma or B-lymphoma cells. Our results indicate that heparanase is produced by B cells during discrete stages of their maturation. We suggest that heparanase may play a role in B-cell migration by enabling pre-B and B lymphocytes to leave the bone-marrow compartment and recirculate among peripheral lymphoid organs.
authors.affiliation: Hubert H. Humphrey Center for Experimental Medicine and Cancer Research, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
authors.forename: R
authors.initials: R
authors.lastname: Laskov
authors.forename: R I
authors.initials: RI
authors.lastname: Michaeli
authors.forename: H
authors.initials: H
authors.lastname: Sharir
authors.forename: E
authors.initials: E
authors.lastname: Yefenof
authors.forename: I
authors.initials: I
authors.lastname: Vlodavsky
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D005109:Extracellular Matrix / Q000378:metabolism; D005966:Glucuronidase; D006026:Glycoside Hydrolases / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D008223:Lymphoma / Q000378:metabolism*; D016393:Lymphoma, B-Cell / Q000378:metabolism; D016399:Lymphoma, T-Cell / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010954:Plasmacytoma / Q000378:metabolism; D000099175:Heparanase
chemical_list: D005966:Glucuronidase; D006026:Glycoside Hydrolases; D008070:Lipopolysaccharides; D000099175:Heparanase