title: The origin of a morphologically unidentifiable human supernumerary minichromosome traced through sorting, molecular cloning, and in situ hybridisation.
abstract: A supernumerary minichromosome has been detected in a severely malformed patient. Attempts at identifying the marker by conventional approaches were unsuccessful. The physical isolation of the minichromosome by fluorescence activated sorting, molecular cloning of its DNA, and in situ hybridisation experiments performed with single copy DNA probes allowed us to show that it was derived from a rearrangement involving the centromere and the proximal region of the short arm of chromosome 9.
authors.affiliation: Dipartimento di Genetica e Microbiologia A Buzzati Traverso, Pavia, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Raimondi
authors.forename: L
authors.initials: L
authors.lastname: Ferretti
authors.forename: B D
authors.initials: BD
authors.lastname: Young
authors.forename: V
authors.initials: V
authors.lastname: Sgaramella
authors.forename: L
authors.initials: L
authors.lastname: De Carli
mesh_terms: D000015:Abnormalities, Multiple / Q000235:genetics*; D002469:Cell Separation; D002503:Centromere; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D015342:DNA Probes; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007231:Infant, Newborn; D009693:Nucleic Acid Hybridization
chemical_list: D015342:DNA Probes; D004247:DNA