Biochemical analysis of glycophospholipids from wild-type mouse lymphoma cells and from Thy-1 negative mutants which do not add glycolipid-anchored proteins.
Thy-1 is a major glycophospholipid (GPL)-anchored protein found on the surface of neurons, epithelial cells, fibroblasts and murine T-lymphomas. Biochemical studies were undertaken to determine if murine T-lymphomas contain glycolipids which may be on the path of GPL-anchor biosynthesis. Biosynthetic labeling experiments on Thy-1-positive (wild-type) cells followed by battery of chemical and enzymatic diagnostics on the isolated [3H]mannolipids have, for the first time, led to description of a set of glycolipids which have properties consistent with their being GPL-anchor precursors. Using these results as a guide, major differences have been observed upon analysis of the radiolabeled mannolipids of Thy-1-negative mutants from 7 complementation classes, A-C, E, F, H and I. The biosynthetic lesions in anchor synthesis have been identified in some of the mutants.
Gupta
D K
DK
Department of Pathology, Case Western Reserve University, School of Medicine, Cleveland, OH 44106.
Lemansky
P A
PA
Tartakoff
A M
AM
D000818:Animals; D000954:Antigens, Surface / Q000096:biosynthesis / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer; D006017:Glycolipids / Q000096:biosynthesis / Q000378:metabolism*; D017261:Glycosylphosphatidylinositols; D016399:Lymphoma, T-Cell / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D010716:Phosphatidylinositols / Q000096:biosynthesis / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D006017:Glycolipids; D017261:Glycosylphosphatidylinositols; D008562:Membrane Glycoproteins; D010716:Phosphatidylinositols; D011134:Polysaccharides; D018800:Thy-1 Antigens