title: G-banding improvement for the MAC method.
abstract: Satisfactory identification of the neoplastic cell as well as an optimal G-banding of its chromosomes are routinely obtained in our laboratory using a modification of the method described by Knuutila et al. Mitotic cells are first identified by either cytochemical or immunological staining. For G-banding, the preparations are then destained with methanol:acetic acid, treated with 2 x SSC at 65 degrees C and restained with Wright stain.
authors.affiliation: Unidad de Hematología y Oncología, Hospital de la Cruz Roja de Barcelona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Pérez
authors.forename: F
authors.initials: F
authors.lastname: Solé
authors.forename: R
authors.initials: R
authors.lastname: Caballín
authors.forename: N
authors.initials: N
authors.lastname: Tarrida
authors.forename: S
authors.initials: S
authors.lastname: Woessner
mesh_terms: D002871:Chromosome Banding / Q000379:methods*; D003584:Cytological Techniques; D006801:Humans; D016130:Immunophenotyping / Q000379:methods