High transformation frequency of nonsporulating mutants of Streptomyces griseus.
Three different bld mutants from S. griseus ATCC 10137 were isolated by nitrosoguanidine mutagenesis. They simultaneously lost the capability of antibiotic production and the formation of pigments. The three bld mutants were differently affected by different carbon sources. Two of these mutants showed a high efficiency of transformation with several plasmid vectors, in contrast to the low efficiency of transformation showed by the wild type. We showed that S. griseus ATCC 10137 and the three bld mutants possess an enzymatic activity that protects their DNAs against the digestion by SacI. Antibiotic and pigment production, and low transformability with plasmid DNA were together restored in spontaneous spo+ revertants.
Daza
A
A
Departamento de Ecología, Facultad de Biología, Universidad de León, Spain.
Martin
J F
JF
Gil
J A
JA
D001681:Biological Assay; D003513:Cycloheximide / Q000096:biosynthesis; D004586:Electrophoresis; D016297:Mutagenesis, Site-Directed; D009604:Nitrosoguanidines / Q000494:pharmacology; D010957:Plasmids; D012150:Polymorphism, Restriction Fragment Length; D013171:Spores, Bacterial; D013305:Streptomyces griseus / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D013307:Streptomycin / Q000096:biosynthesis; D014169:Transformation, Bacterial
D009604:Nitrosoguanidines; D003513:Cycloheximide; D013307:Streptomycin