title: Immunological characterization of Clostridium butyricum neurotoxin and its trypsin-induced fragment by use of monoclonal antibodies against Clostridium botulinum type E neurotoxin.
abstract: We examined the reactivities of Clostridium butyricum neurotoxin to nine monoclonal antibodies against Clostridium botulinum type E neurotoxin which recognize the light chain or the amino-terminal half (H-1 fragment) or the carboxyl-terminal half (H-2 fragment) of the heavy chain of botulinum neurotoxin. Butyricum neurotoxin and its derived chains did not react to two of four monoclonal antibodies recognizing the light chain, one of three recognizing the H-1 fragment, and one of two recognizing the H-2 fragment. The results indicate that the immunological difference between the two neurotoxins is not attributable to a particular portion of the toxin molecule. The fragment of butyricum neurotoxin obtained by prolonged tryptic treatment was found to comprise the light chain and H-1 fragment linked together by a disulfide bond.
authors.affiliation: Department of Veterinary Science, College of Agriculture, University of Osaka Prefecture, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kozaki
authors.forename: J
authors.initials: J
authors.lastname: Onimaru
authors.forename: Y
authors.initials: Y
authors.lastname: Kamata
authors.forename: G
authors.initials: G
authors.lastname: Sakaguchi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001905:Botulinum Toxins / Q000276:immunology*; D003013:Clostridium / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D009498:Neurotoxins / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology*; D014357:Trypsin / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D009498:Neurotoxins; D010446:Peptide Fragments; D014357:Trypsin; D001905:Botulinum Toxins