Deletion of 55 open reading frames from the termini of vaccinia virus.
Each copy of the inverted terminal repeat of vaccinia virus consists of 8 kb of DNA containing 9 ORFS flanked near the terminus of the genome by 4 kb of repetitive DNA which in turn contains blocks of tandem repeats. Using plasmids containing repetitive DNA as the external arm, we have generated deletions at both the left and the right termini of the vaccinia genome. We report here the engineered deletion within a single vaccinia virus of 32.7 kb of DNA (including 38 ORFS) from the left terminus and 14.9 kb of DNA (including 17 ORFS) from the right terminus.
Perkus
M E
ME
Virogenetics Corporation, Rensselaer Technology Park, Troy, New York 12180.
Goebel
S J
SJ
Davis
S W
SW
Johnson
G P
GP
Norton
E K
EK
Paoletti
E
E
D001483:Base Sequence; D015139:Blotting, Southern; D002872:Chromosome Deletion; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016366:Open Reading Frames; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D014616:Vaccinia virus / Q000235:genetics* / Q000502:physiology
D004279:DNA, Viral