title: Interaction of ATP with acetyl-CoA carboxylase from rat liver. The role of the polyphosphate chain. Affinity labelling with alkylating amides of ATP and ADP.
abstract: The interaction of a number of ATP analogs with a modified triphosphate moiety as well as 2-chloro-ethyl-amino derivatives of nucleotides, 4(N-2-chloroethyl-N-methylamino)-benzyl-gamma-amide of ATP and the corresponding ADP beta-amide with acetyl-CoA carboxylase (EC 6.4.1.2.) from rat liver has been studied. Halophosphonate derivatives of ATP have been synthesized from bromomethylene diphosphonic acid and found to be reversible inhibitors of the enzyme. ATP and ADP alkylating amides have proved to form a reversible complex with the ATP-binding site and to modify a group in the acetyl-CoA-binding site. The bicarbonate ion accelerates the process of inactivation. The estimate of the distance between the ATP-binding site and the acetyl-CoA-binding site ranges within 0.8-1.2 nm.
authors.affiliation: VA Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
authors.forename: A G
authors.initials: AG
authors.lastname: Rabinkov
authors.forename: S V
authors.initials: SV
authors.lastname: Amontov
authors.forename: V N
authors.initials: VN
authors.lastname: Buneva
authors.forename: N B
authors.initials: NB
authors.lastname: Tarussova
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000037:antagonists & inhibitors*; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000378:metabolism; D000345:Affinity Labels; D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology; D001710:Biotin / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008958:Models, Molecular; D051381:Rats; D013379:Substrate Specificity
chemical_list: D000345:Affinity Labels; D001639:Bicarbonates; D001710:Biotin; D000255:Adenosine Triphosphate; D000103:Acetyl-CoA Carboxylase