Recommended protocols based on a survey of current practice in genotoxicity testing laboratories: I. Unscheduled DNA synthesis assay in rat hepatocyte cultures.
A protocol based primarily on current laboratory practices in the performance of the unscheduled DNA synthesis (UDS) assay with primary rat hepatocyte cultures has been developed. These guidelines were developed using tabulated responses to a detailed questionnaire completed by North American and European governmental, university and contract laboratories involved with the UDS test. This report identifies those modifications to previously described methodologies which are used on a regular basis and also serves to clarify confusing or inconsistent practices. Although this protocol pertains specifically to the use of primary rat hepatocyte cultures, it can be modified to incorporate other types of cells in which certain aspects remain the same.
Swierenga
S H
SH
Bureau of Drug Research, Health Protection Branch, Health and Welfare Canada, Ottawa, Ont. Canada.
Bradlaw
J A
JA
Brillinger
R L
RL
Gilman
J P
JP
Nestmann
E R
ER
San
R C
RC
D000818:Animals; D002478:Cells, Cultured; D004260:DNA Repair; D004261:DNA Replication / Q000187:drug effects*; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D009152:Mutagenicity Tests / Q000379:methods*; D051381:Rats; D012107:Research Design