title: Macrophage antigen presentation and interleukin 1 production after cecal ligation and puncture in C3H/HeN and C3H/HeJ mice.
abstract: Following cecal ligation and puncture with a 25-gauge needle, endotoxin-sensitive C3H/HeN mice have a 45% mortality compared with no mortality in endotoxin-resistant C2H/HeJ mice. Macrophage production of interleukin 1 and antigen presentation were studied in these two strains of mice following cecal ligation and puncture at 2, 4, 8, 16, and 24 hours and at 2, 4, 6, and 8 days. Splenic macrophages were cultured with a T-helper cell clone (D10.G4.1), and antigen presentation and interleukin 1 production were measured by D10.G4.1 proliferation. Macrophage antigen presentation by C2H/HeJ mice was markedly increased compared with that in C3H/HeN mice at all times after cecal ligation and puncture, most strikingly at 2 days (185m740 cpm for C3H/HeJ mice vs 30,300 for C2H/HeN mice). Macrophage interleukin 1 production was significantly increased in C3H/HeJ mice vs C3H/HeN mice at all times after cecal ligation and puncture (except at 2 days) and was maximal at 8 days (25,000 cpm for C3H/HeJ mice vs 5190 for C3H/HeN mice). These data suggest that the differences in mortality after cecal ligation and puncture between these two strains of mice may relate to a supranormal response of macrophages of C3H/HeJ mice or to an inadequate response of macrophages of C3H/HeN mice.
authors.affiliation: Department of Surgery, University of North Carolina School of Medicine, Chapel Hill.
authors.forename: C C
authors.initials: CC
authors.lastname: Baker
authors.forename: A T
authors.initials: AT
authors.lastname: Niven-Fairchild
authors.forename: A
authors.initials: A
authors.lastname: Yamada
authors.forename: C L
authors.initials: CL
authors.lastname: Caragnano
authors.forename: T S
authors.initials: TS
authors.lastname: Kupper
mesh_terms: D000818:Animals; D001424:Bacterial Infections / Q000276:immunology* / Q000378:metabolism; D004198:Disease Susceptibility; D004731:Endotoxins; D007375:Interleukin-1 / Q000096:biosynthesis*; D016177:Macrophage-1 Antigen / Q000032:analysis*; D008264:Macrophages / Q000276:immunology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D013154:Spleen / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D004731:Endotoxins; D007375:Interleukin-1; D016177:Macrophage-1 Antigen