Characterization of the class III region in different MHC haplotypes by pulsed-field gel electrophoresis.
The class III region of the human major histocompatibility complex (MHC) in seven HLA haplotypes has been analyzed using pulsed-field gel electrophoresis (PFGE), restriction enzymes that cut genomic DNA infrequently, and Southern blotting. In particular, extensive mapping with the enzyme Bss HII, which generates fragments in the size range 8-280 kilobases (kb), has revealed that in the haplotypes studied the DNA content of the class III region does not appear to vary other than as previously observed at the C4 and CYP21 loci.
Dunham
I
I
MRC Immunochemistry Unit, Department of Biochemistry, Oxford, United Kingdom.
Sargent
C A
CA
Kendall
E
E
Campbell
R D
RD
D015139:Blotting, Southern; D004587:Electrophoresis, Agar Gel; D006680:HLA Antigens / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D008285:Major Histocompatibility Complex; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D006680:HLA Antigens