Formaldehyde cross-linking and immunoprecipitation demonstrate developmental changes in H1 association with transcriptionally active genes.
The in vivo association of histone H1 with specific genes in Tetrahymena thermophila was studied by using a simplified cross-linking and immunoprecipitation technique. Four genes were analyzed whose activities vary in three different developmental states (logarithmic growth, starvation, and conjugation). Hybridization of the immunoprecipitated DNA to cloned probes showed an inverse correlation between the level of immunoprecipitation with H1 antiserum and transcriptional activity. This represents the first demonstration of an alteration in histone H1-DNA interaction associated with developmental changes in transcriptional activity.
Dedon
P C
PC
Department of Biology, University of Rochester, New York 14627.
Soults
J A
JA
Allis
C D
CD
Gorovsky
M A
MA
D000199:Actins / Q000235:genetics; D000818:Animals; D003227:Conjugation, Genetic; D003432:Cross-Linking Reagents; D004275:DNA, Ribosomal / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005557:Formaldehyde; D015870:Gene Expression; D006657:Histones / Q000378:metabolism*; D009707:Nucleosomes / Q000648:ultrastructure; D011233:Precipitin Tests; D013768:Tetrahymena / Q000235:genetics*; D014158:Transcription, Genetic
D000199:Actins; D003432:Cross-Linking Reagents; D004275:DNA, Ribosomal; D004268:DNA-Binding Proteins; D006657:Histones; D009707:Nucleosomes; D005557:Formaldehyde