Studies on the bovine major histocompatibility class I and class II antigens using homozygous typing cells and antigen-specific BoT4+ blast cells.
Animals were identified from two sire lines as being homozygous for the class I bovine lymphocyte antigen (BoLA-A) w23. These animals were also shown to be homozygous for class II antigens (BoLA-D) which, however, differed between the two sire lines. Lymphocytes from these animals were then used either as stimulator cells in one-way mixed lymphocyte reactions (MLR) with all animals in the herd carrying the w23 antigen or as antigen presenting cells to bovine T4+ cell blasts. It was shown that, within each sire line, the genes encoding the MHC class I and class II antigens were closely linked. There were no detected recombinations between the MHC class I and class II regions nor within the BoLA-D region responsible for mixed lymphocyte reactivity. MLR typing of MHC class II antigens correlated with the results from T-lymphocyte proliferation studies. Cells from these cattle, which are homozygous at the class I and II MHC loci but differ in the class II antigen expressed, could be used to type the BoLA-D of other cattle.
Rothel
J S
JS
CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Victoria, Australia.
Dufty
J H
JH
Wood
P R
PR
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002417:Cattle / Q000235:genetics / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D008040:Genetic Linkage; D015395:Histocompatibility Antigens Class I / Q000032:analysis / Q000235:genetics*; D000949:Histocompatibility Antigens Class II / Q000032:analysis / Q000235:genetics*; D007959:Lymphocyte Culture Test, Mixed
D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II