Inhibition of gamma-glutamyl transpeptidase decreases amino acid uptake in human keratinocytes in culture.
Acivicin inhibits gamma-glutamyl transpeptidase activity in human keratinocytes in culture. Treatment of these cells with acivicin produces a decrease in the uptake of L-[U-14C]alanine, 2-amino-[1-14C]-isobutyrate, L-[U-14C]leucine and 1-aminocyclopentane-1-[14C]carboxylate. D-[U-14C]glucose uptake is not affected by the presence of acivicin. These results support, for the first time in vitro, the hypothesis that the gamma-glutamyl cycle may be involved in amino acid uptake by human cells.
Viña
J R
JR
Departamento de Bioquímica-Biología Molecular, Facultad de Medicina, Universitat de Valencia, Spain.
Blay
P
P
Ramirez
A
A
Castells
A
A
Viña
J
J
D000596:Amino Acids / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D002478:Cells, Cultured; D005947:Glucose / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007555:Isoxazoles / Q000494:pharmacology; D015603:Keratinocytes / Q000201:enzymology* / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000037:antagonists & inhibitors*
D000596:Amino Acids; D007555:Isoxazoles; D005723:gamma-Glutamyltransferase; D005978:Glutathione; D005947:Glucose; C006303:acivicin