title: Cleavage and purification of prokaryotically expressed HIV gag and env fusion proteins for detection of HIV antibodies in the ELISA.
abstract: Parts of the gag p24 and the gp41 transmembrane protein of the human immunodeficiency virus HIV-1 were expressed as fusion proteins in Escherichia coli, using an expression vector carrying aa 1-375 of the lac-Z gene linked to the recognition sequence for the blood coagulation factor Xa. Fusion proteins were cleaved into the bacterial and viral portion and the viral polypeptide was purified by a molecular sieve column. The purified viral antigens were tested with 288 human sera in the enzyme-linked immunosorbent assay (ELISA) technique. Comparison with commercially available tests showed comparable sensitivity and a higher specificity of the gag/env-ELISA for borderline reactive sera.
authors.affiliation: Institut für Klinische und Molekulare Virologie, Universität Erlangen-Nürnberg, Federal Republic of Germany.
authors.forename: S
authors.initials: S
authors.lastname: Ellinger
authors.forename: M
authors.initials: M
authors.lastname: Mach
authors.forename: K
authors.initials: K
authors.lastname: Korn
authors.forename: G
authors.initials: G
authors.lastname: Jahn
mesh_terms: D002850:Chromatography, Gel; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics; D015686:Gene Products, env / Q000276:immunology* / Q000302:isolation & purification; D015683:Gene Products, gag / Q000276:immunology* / Q000302:isolation & purification; D015483:HIV Antibodies / Q000032:analysis*; D006679:HIV Seropositivity; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D014760:Viral Fusion Proteins / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D015686:Gene Products, env; D015683:Gene Products, gag; D015483:HIV Antibodies; D014760:Viral Fusion Proteins