Differential expression of the mouse D2 dopamine receptor isoforms.
We have identified and characterized the cDNAs corresponding to the mouse D2 dopamine receptors. We show that in the mouse the D2 dopamine receptor is found in two forms, generated by alternative splicing of the same gene, mRNA distribution analysis of areas expressing the D2 receptors shows that the larger form is the most abundant, except in the brain stem where the shorter form is predominant. Membranes of mammalian cells transiently transfected with both forms of D2 receptor bind [3H]spiperone with a high affinity.
Montmayeur
J P
JP
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Génie Génétique et de Biologie Moléculaire INSERM, Institut de Chimie Biologique Faculté de Médecine, Strasbourg, France.
Bausero
P
P
Amlaiky
N
N
Maroteaux
L
L
Hen
R
R
Borrelli
E
E
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D001921:Brain / Q000502:physiology*; D001931:Brain Mapping; D001971:Bromocriptine / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D011954:Receptors, Dopamine / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D013134:Spiperone / Q000378:metabolism
D012333:RNA, Messenger; D011954:Receptors, Dopamine; D011994:Recombinant Proteins; D001971:Bromocriptine; D013134:Spiperone; D004247:DNA