title: Constitutive expression of a groEL-related protein on the surface of human gamma/delta cells.
abstract: Rabbit antibodies to hsp58 (P1), the human homologue of the Escherichia coli stress protein groEL, react specifically in indirect immunofluorescence and complement-dependent microcytoxicity experiments with a cell surface antigen expressed constitutively by T cell lines bearing gamma/delta receptors. This anti-hsp58-reactive antigen is not demonstrable on T cells that express alpha/beta receptors or on various cells that lack T cell receptors. Certain evidence was obtained to suggest that the target antigen on the surface of gamma/delta T cells is a approximately 77-kD protein distinct from intracellular hsp58 and known members of the hsp70 stress protein family. While the exact nature and significance of this anti-hsp58-reactive protein remain to be determined, these data may help to clarify the roles of groEL-related stress proteins and gamma/delta cells that recognize groEL homologous in immunologic defense against infection and in autoimmune disease.
authors.affiliation: Division of Rheumatology and Immunology, University of North Carolina, Chapel Hill 27514.
authors.forename: W
authors.initials: W
authors.lastname: Jarjour
authors.forename: L A
authors.initials: LA
authors.lastname: Mizzen
authors.forename: W J
authors.initials: WJ
authors.lastname: Welch
authors.forename: S
authors.initials: S
authors.lastname: Denning
authors.forename: M
authors.initials: M
authors.lastname: Shaw
authors.forename: T
authors.initials: T
authors.lastname: Mimura
authors.forename: B F
authors.initials: BF
authors.lastname: Haynes
authors.forename: J B
authors.initials: JB
authors.lastname: Winfield
mesh_terms: D000906:Antibodies; D000954:Antigens, Surface / Q000032:analysis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D002460:Cell Line; D002478:Cells, Cultured; D018834:Chaperonin 60; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005455:Fluorescent Antibody Technique; D006360:Heat-Shock Proteins / Q000032:analysis / Q000235:genetics*; D006801:Humans; D015151:Immunoblotting; D008970:Molecular Weight; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000906:Antibodies; D000954:Antigens, Surface; D001426:Bacterial Proteins; D018834:Chaperonin 60; D006360:Heat-Shock Proteins; D011948:Receptors, Antigen, T-Cell