Molecular and phenotypic variation of the Zw locus region in Drosophila melanogaster.
Restriction map polymorphism in a 13-kb region of the Zw locus in Drosophila melanogaster was investigated for 64 X chromosome lines with seven 6-cutter and ten 4-cutter restriction enzymes. A total of 203 restriction sites were scored, of which 20 were found to be polymorphic. The estimated nucleotide variation for this region for overall data (pi = 0.003 and 0.001, and theta = 0.003 and 0.002, for 4-cutter and 6-cutter studies, respectively) was smaller than that reported for most regions studied in D. melanogaster. It was found that the Slow allozyme has a larger nucleotide variation and haplotype diversity than the Fast allozyme. Results suggest the relatively recent divergence of the Fast allozyme from the Slow allozyme. Glucose 6-phosphate dehydrogenase (G6PD) activity was measured as a phenotype of the Zw locus. A significant difference in G6PD activity between allozymes was detected. The between-line effect was highly significant within the Slow allozyme, but was not significant within the Fast allozyme. Although a direct causative link could not be established, these results suggest an association between the amounts of quantitative and molecular genetic variation at the Zw locus region.
Miyashita
N T
NT
Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
D000704:Analysis of Variance; D000818:Animals; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D006239:Haplotypes; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D015810:Linkage Disequilibrium; D008297:Male; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic; D015183:Restriction Mapping; D014960:X Chromosome
D007527:Isoenzymes; D005954:Glucosephosphate Dehydrogenase