title: Effect of intraosseous saline infusion on hematologic parameters.
abstract: OBJECTIVE
  To examine the effects of intraosseous saline infusion on hematologic parameters.
  
  
  METHODS
  Eight New Zealand White rabbits were anesthetized with intramuscular ketamine. An initial blood sample was withdrawn from a earlobe vein on induction of anesthesia. An 18-gauge intraosseous needle was then inserted into the rabbit's proximal tibia, and 10 mL/kg of normal saline was infused over one minute. Two additional blood samples were obtained 15 and 30 minutes after the intraosseous infusion.
  
  
  METHODS
  Complete blood counts were performed on the blood samples.
  
  
  RESULTS
  Significant differences were found in monocytes, basophils, and nucleated RBCs from samples drawn before and after intraosseous infusion. The microscopic examination of the peripheral blood smear revealed an increase in the number of burr cells, schistocytes, and polychromasia in the postinfusion samples.
  
  
  CONCLUSIONS
  We advocate caution in the interpretation of hematologic studies performed on blood samples obtained shortly after an intraosseous infusion.
authors.affiliation: Department of Pediatrics, Loyola University Medical Center, Maywood, Illinois 60153.
authors.forename: S P
authors.initials: SP
authors.lastname: Ros
authors.forename: S I
authors.initials: SI
authors.lastname: McMannis
authors.forename: A
authors.initials: A
authors.lastname: Kowal-Vern
authors.forename: W P
authors.initials: WP
authors.lastname: Zeller
authors.forename: R M
authors.initials: RM
authors.lastname: Hurley
mesh_terms: D000818:Animals; D001772:Blood Cell Count / Q000187:drug effects*; D001842:Bone and Bones; D004912:Erythrocytes / Q000187:drug effects / Q000473:pathology; D005069:Evaluation Studies as Topic; D007263:Infusions, Parenteral / Q000592:standards*; D007958:Leukocyte Count / Q000187:drug effects; D011817:Rabbits; D012965:Sodium Chloride / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: D012965:Sodium Chloride