Products and intermediates of the beta-oxidation of [U-14C]hexadecanedionoyl-mono-CoA by rat liver peroxisomes and mitochondria.
1. The synthesis of [U-14C]hexadecanedionoyl-mono-CoA is described. 2. The beta-oxidation of [U-14C]hexadecanedionoyl-mono-CoA by purified rat liver peroxisomes and mitochondria is demonstrated. 3. The products of mitochondrial beta-oxidation of [U-14C]hexadecanedionoyl-mono-CoA include ketone bodies, citrate and acetylcarnitine. 4. Tetradecadionoyl-mono-CoA, hexadec-2-enedionyl-mono-CoA and hexadionoyl-mono-CoA were the only detectable intermediates formed by mitochondrial beta-oxidation, whereas acetyl-CoA and all saturated even-numbered intermediates of chain length C6-C16 were generated by peroxisomal beta-oxidation. 5. Hexadecanedionoyl-mono-CoA and hexadecanoyl-CoA were equally effective substrates for peroxisomal beta-oxidation, but hexadecanedionoyl-mono-CoA was a relatively poorer substrate for the mitochondrial pathway.
Pourfarzam
M
M
Department of Child Health, Medical School, University of Newcastle upon Tyne, U.K.
Bartlett
K
K
D000108:Acetylcarnitine / Q000737:chemistry; D000214:Acyl Coenzyme A / Q000737:chemistry*; D000818:Animals; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid; D002951:Citrates / Q000737:chemistry; D006868:Hydrolysis; D007657:Ketone Bodies / Q000737:chemistry; D008099:Liver / Q000378:metabolism*; D008297:Male; D008830:Microbodies / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains
D000214:Acyl Coenzyme A; D002250:Carbon Radioisotopes; D002951:Citrates; D007657:Ketone Bodies; C066987:hexadecanedionyl-mono-coenzyme A; D000108:Acetylcarnitine