title: Inactivation of gastric and pancreatic lipases by diethyl p-nitrophenyl phosphate.
abstract: Reacting gastric and pancreatic lipases with mixed diethyl p-nitrophenyl phosphate/bile salt micelles resulted in a stoichiometric inactivation of these enzymes as tested on emulsified tributyroylglycerol and trioleoylglycerol as substrates. Diethyl p-nitrophenyl phosphate treated gastric lipases were also inactive on water-soluble p-nitrophenyl acetate, whereas the modified pancreatic lipase was still able to hydrolyze this water-soluble substrate. The binding of diethyl p-nitrophenyl phosphate modified pancreatic and gastric lipases to tributyroylglycerol/water interface was comparable to that of native lipases. The essential free sulfhydryl group of gastric lipases underwent no chemical changes due to the reaction with micellar diethyl p-nitrophenyl phosphate. All in all, these results indicate that, in both gastric and pancreatic lipases, the essential serine residue which was stoichiometrically labeled by this organophosphorus reagent is involved in catalysis and not in lipid binding.
authors.affiliation: Centre de Biochimie et de Biologie Moléculaire, Centre National de la Recherche Scientifique (CNRS), Marseille, France.
authors.forename: H
authors.initials: H
authors.lastname: Moreau
authors.forename: A
authors.initials: A
authors.lastname: Moulin
authors.forename: Y
authors.initials: Y
authors.lastname: Gargouri
authors.forename: J P
authors.initials: JP
authors.lastname: Noël
authors.forename: R
authors.initials: R
authors.lastname: Verger
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002250:Carbon Radioisotopes; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D008823:Micelles; D008969:Molecular Sequence Data; D009596:Nitrophenols / Q000378:metabolism; D010184:Pancreatic Extracts / Q000378:metabolism*; D020799:Pancrelipase; D010261:Paraoxon / Q000494:pharmacology*; D011817:Rabbits; D013379:Substrate Specificity; D014304:Triolein / Q000378:metabolism
chemical_list: D002250:Carbon Radioisotopes; D008823:Micelles; D009596:Nitrophenols; D010184:Pancreatic Extracts; D014304:Triolein; D020799:Pancrelipase; C008642:4-nitrophenyl acetate; D008049:Lipase; D010261:Paraoxon