A rapid method for detecting DNA strand breaks in Mytilus galloprovincialis Lam. induced by genotoxic xenobiotic chemicals.
1. In this study, DNA from haemolymph cells of Mytilus galloprovincialis Lam., as well as from L1210 (murine leukemia) mouse cells was investigated utilizing the technique of the alkaline unwinding of the double stranded DNA molecule. 2. The data show that DNA of haemolymph cells from the marine invertebrate has an unwinding time and, therefore, a molecular weight considerably lower than that of DNA of mammalian cells. 3. The exposure of the cells from mussel haemolymph and from mouse L1210 to a genotoxic compound such as dimethylsulfate results in DNA damage and consequently in a reduction of the unwinding time. 4. These results suggest that the fluorimetric DNA unwinding assay can be used in studies concerning the damage of DNA of marine organisms induced by genotoxic compounds or environmental factors.
Accomando
R
R
Istituto di Fisiologia Generale, Universitá di Genova, Italia.
Viarengo
A
A
Bordone
R
R
Taningher
M
M
Canesi
L
L
Orunesu
M
M
D000818:Animals; D049872:Bivalvia / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000187:drug effects*; D004249:DNA Damage; D006458:Hemolymph / Q000737:chemistry; D007700:Kinetics; D007939:Leukemia L1210; D009153:Mutagens / Q000494:pharmacology*; D009690:Nucleic Acid Conformation / Q000187:drug effects; D013463:Sulfuric Acid Esters / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D009153:Mutagens; D013463:Sulfuric Acid Esters; D004247:DNA; C007482:dimethyl sulfate