title: In vitro inhibition of cell growth of MOLT-4 malignant human T-lymphoblasts by coenzyme F420.
abstract: The inhibitory effect of methanogenic coenzymes on the proliferation of MOLT-4 human malignant T-lymphoblasts was tested. Furthermore the effects of methanogenic coenzymes on dihydrofolate reductase activity (DHFR) from chicken liver have been examined. The results showed that heat-stable extracts of the hydrogenotrophs Methanobacterium thermoautotrophicum, Methanoculleus thermophilicum and Methanogenium tationis inhibit both proliferation of human T-lymphoblasts and DHFR activity. Heat-stable extract of the methylotroph Methanosarcina barkeri showed neither inhibitory nor stimulatory effects in both test systems. The present study proves coenzyme F420 to be the active, inhibitory component in methanogenic extracts.
authors.affiliation: Department of Microbiology, Faculty of Science, University of Nijmegen, The Netherlands.
authors.forename: P C
authors.initials: PC
authors.lastname: Raemakers-Franken
authors.forename: R A
authors.initials: RA
authors.lastname: De Abreu
authors.forename: J G
authors.initials: JG
authors.lastname: Willems
authors.forename: C
authors.initials: C
authors.lastname: van der Drift
authors.forename: G D
authors.initials: GD
authors.lastname: Vogels
mesh_terms: D002455:Cell Division / Q000187:drug effects*; D019605:Euryarchaeota / Q000502:physiology; D005493:Folic Acid Antagonists; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D008727:Methotrexate / Q000494:pharmacology; D011622:Pterins / Q000494:pharmacology; D012256:Riboflavin / Q000031:analogs & derivatives* / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D005493:Folic Acid Antagonists; D011622:Pterins; C015084:coenzyme F420; C036686:methanopterin; D012256:Riboflavin; D008727:Methotrexate