Effects of uric acid in aqueous phase on neutrophil alkaline phosphatase.
In vitro effects of uric acid (2-6-8 trioxypurine) on purified human neutrophil alkaline phosphatase were studied. A marked activation of the enzyme catalyzed reaction is observed. This activation is dose and pH dependent and not influenced by dialysis. Uric acid can form a stable complex with alkaline phosphatase, whose biochemical characteristics (heat stability, reactivity towards some inhibitors) are significantly modified.
Vergnes
H A
HA
Laboratoire d'Enzymologie du Granulocyte, INSERM, CNRS, Toulouse, France.
Grozdea
J
J
Brisson-Lougarre
A
A
D000328:Adult; D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004789:Enzyme Activation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009504:Neutrophils / Q000201:enzymology*; D013696:Temperature; D014527:Uric Acid / Q000494:pharmacology*
D014527:Uric Acid; D000469:Alkaline Phosphatase