Insulin absorption from conjunctiva studied in normal and diabetic dogs.
The dynamics of insulin absorption from the ocular conjunctiva of anaesthetized normal and pancreatectomized dogs have been examined. A porcine insulin preparation of 1000 units mL-1 (pH 8.0) was administered as either 1 or 10 units kg-1 to the upper conjunctival sacs of recumbent dogs following an overnight fast. Plasma insulin concentrations increased significantly at 5 min after the insulin administration. Plasma glucose concentrations decreased significantly, in both normal (given 10 units kg-1) and diabetic dogs (given 1 unit kg-1 or 10 units kg-1). There was a dose-dependent increase in plasma insulin concentration following conjunctival administration. Estimated absorption was significantly higher in diabetic than in normal dogs.
Nomura
M
M
Department of Medicine, Osaka University School of Medicine, Japan.
Kubota
M A
MA
Sekiya
M
M
Hoshiyama
S
S
Imano
E
E
Matushima
Y
Y
Ishimoto
I
I
Kawamori
R
R
Kamada
T
T
D000042:Absorption; D000818:Animals; D001682:Biological Availability; D001786:Blood Glucose / Q000378:metabolism; D003228:Conjunctiva / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000097:blood*; D004285:Dogs; D007328:Insulin / Q000097:blood / Q000493:pharmacokinetics*
D001786:Blood Glucose; D007328:Insulin