Fibroblast growth factor gene expression in AIDS-Kaposi's sarcoma detected by in situ hybridization.
Biopsy samples from five acquired immune deficiency syndrome (AIDS)-Kaposi's sarcomas and one non-AIDS-associated Kaposi's sarcoma were assayed by in situ RNA hybridization onto paraformaldehyde-fixed, paraffin-embedded skin sections for the presence of two fibroblast growth factor gene transcripts, FGFB and FGF5. FGF5 gene expression was detected in the characteristic Kaposi's sarcoma spindle-shaped cells in the five samples from human immunodeficiency-positive (HIV+) patients. FGFB transcripts were detected in Kaposi's sarcoma cells as well as in epidermis of HIV- and HIV+ patients. These results complement the observations about growth factor gene expression done on Kaposi's sarcoma-derived cell lines, which thus appear to be representative of what happens in vivo. Furthermore, they demonstrate a contrasting expression pattern of FGF5 and FGFB genes, both involved in the growth factor pathogenic cascade leading to Kaposi's sarcoma.
Xerri
L
L
Laboratoire d'Anatomo-pathologie, Institut Paoli-Calmettes, U119 INSERM, Marseille, France.
Hassoun
J
J
Planche
J
J
Guigou
V
V
Grob
J J
JJ
Parc
P
P
Birnbaum
D
D
deLapeyriere
O
O
D000163:Acquired Immunodeficiency Syndrome / Q000235:genetics*; D005346:Fibroblast Growth Factors / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D015967:Gene Expression Regulation, Viral; D006801:Humans; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D016372:RNA, Antisense; D012514:Sarcoma, Kaposi / Q000235:genetics*
D015347:RNA Probes; D016372:RNA, Antisense; D005346:Fibroblast Growth Factors