The energy state of tumor-bearing rats.
Rats bearing the Walker-256 carcinosarcoma have a profoundly altered liver metabolite content with significant increases in the concentrations of glucose 6-phosphate, fructose 1,6-bisphosphate, citrate, lactate, and alanine, while the concentrations of glucose, pyruvate, dihydroxyacetone phosphate, and glutamine are decreased. As a result of these changes both the cytosolic NAD+/NADH ratio and the cytosolic phosphorylation potential are significantly lowered while no changes are detected in either the cytosolic NADP+/NADPH ratio or the mitochondrial NAD+/NADH ratio. These hepatic changes are accompanied by marked increases in the circulating concentrations of lactate, non-esterified fatty acids, and triacylglycerols. The activities of both liver hexokinase and phosphofructokinase are also significantly elevated in the tumor-bearing rats. The changes observed both in the redox state and phosphorylation potential are in agreement with the energy imbalance associated with tumor burden.
Argilés
J M
JM
Departament de Bioquímica i Fisiologia, Facultat de Biologia, Universitat de Barcelona, Spain.
López-Soriano
F J
FJ
D000818:Animals; D002279:Carcinoma 256, Walker / Q000378:metabolism*; D004734:Energy Metabolism; D005260:Female; D008099:Liver / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism*; D009368:Neoplasm Transplantation; D010084:Oxidation-Reduction; D010766:Phosphorylation; D051381:Rats; D011919:Rats, Inbred Strains