Expression of LFA-1 by a lymphoblastoid cell line from a patient with monosomy 21: effects on intercellular adhesion.
Monosomy 21 (M21) is a rare aneuploid condition which in certain cases leads to reduced levels of chromosome 21 gene products. We have prepared an Epstein-Barr virus lymphoblastoid cell-line (LCL) from patient with M21 who has immunological abnormalities, and analysed the expression of lymphocyte function-associated antigen-1 (LFA-1). This heterodimeric leucocyte integrin consists of CD11a (alpha) subunits non-covalently associated with CD18 (beta) subunits coded, respectively, by genes on chromosomes 16 and 21. To determine whether monosomy 21 results in decreased expression of LFA-1, monoclonal antibodies were used to compare the expression of CD11a and CD18 on the M21 LCL with LCL from trisomy 21 (Down's syndrome, T21), normal controls and a possible case of leucocyte adhesion deficiency. In addition, phorbol-ester-induced homotypic adhesion, an LFA-1-mediated effect, was compared in these LCLs. The results are consistent with a gene dosage mediated reduction of LFA-1 expression by the M21 LCL.
Taylor
G M
GM
Immunogenetics Laboratory, St Mary's Hospital, Manchester, UK.
Braddock
D
D
Robson
A J
AJ
Fergusson
W D
WD
Duckett
D P
DP
D'Souza
S W
SW
Brenchley
P
P
D015703:Antigens, CD / Q000378:metabolism*; D000943:Antigens, Differentiation / Q000378:metabolism*; D018821:CD18 Antigens; D002448:Cell Adhesion; D002460:Cell Line; D002472:Cell Transformation, Viral / Q000276:immunology; D002872:Chromosome Deletion; D002891:Chromosomes, Human, Pair 21; D004314:Down Syndrome / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1; D008214:Lymphocytes / Q000276:immunology* / Q000473:pathology; D009006:Monosomy; D016028:Receptors, Leukocyte-Adhesion / Q000378:metabolism*
D015703:Antigens, CD; D000943:Antigens, Differentiation; D018821:CD18 Antigens; D016169:Lymphocyte Function-Associated Antigen-1; D016028:Receptors, Leukocyte-Adhesion