Sequence analysis of the human thymidine kinase gene promoter: comparison with the human PCNA promoter.
We report the sequence of 4264 nucleotides of 5' flanking sequence of the human thymidine kinase gene, a gene that is maximally expressed at the G1/S boundary of the cell cycle. The position of nucleotide sequences which can act as binding sites for trans-acting factors, Sp-1, AP-1/jun, AP-2, OTF-1 and CAAT box factors as well as other potential cis-acting sequences have been mapped. The organization of these cis-acting sequences in the promoter of the human PCNA gene (another gene that is maximally expressed at the G1/S boundary) are shown for comparison. The potential role that these sequences may play in the transcriptional regulation of these genes is discussed.
Sauvé
G J
GJ
Department of Pathology, Temple University School of Medicine, Philadelphia, PA 19140.
Lipson
K E
KE
Chen
S T
ST
Baserga
R
R
D001483:Base Sequence; D001665:Binding Sites; D004247:DNA; D006657:Histones / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D018809:Proliferating Cell Nuclear Antigen; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D016385:TATA Box; D013937:Thymidine Kinase / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism
D006657:Histones; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D014157:Transcription Factors; D004247:DNA; D013937:Thymidine Kinase