Kinetics of the intestinal uptake of zinc acexamate in normal and zinc-depleted rats.
The uptake of zinc as acexamic acid salt in the small intestine of the anaesthetized rat was shown to be a two-phase process in normal animals. The first phase is rapid mucosal binding which satisfies the Freundlich isotherm equation and which involves about 30 per cent of the initially perfused zinc. The second phase was characterized as an apparent absorption step which obeys Michaelis-Menten and first-order combined kinetics, with the following parameters: Vm = 6.51 mg h-1; Km = 2.96 mg; ka = 0.306 h-1. In largely non-saturated conditions, an apparent global rate constant of about 2.50 h-1 was calculated. No significant interference due to endogenous zinc excretion into the small intestine was observed during the absorption period. In zinc-deficient animals, the two phases were not so well characterized. Binding was non-linear and apparent absorption efficiency was much greater at high zinc concentrations, so no evidence of saturable kinetics was found, thus confirming the hypothesis of a homeostatic zinc regulation mechanism.
Torres-Molina
F
F
Department of Pharmacology and Pharmaceutics, University of Valencia, Spain.
Martínez-Coscollá
A
A
Gisbert
S
S
Quintana
E
E
Sendrós
S
S
Peris-Ribera
J E
JE
Plá-Delfina
J M
JM
D000614:Aminocaproates; D015119:Aminocaproic Acid / Q000493:pharmacokinetics; D000818:Animals; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism; D008297:Male; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D013054:Spectrophotometry, Atomic; D015032:Zinc / Q000172:deficiency* / Q000652:urine
D000614:Aminocaproates; C018802:6-acetylaminocaproic acid; D015032:Zinc; D015119:Aminocaproic Acid