Restriction fragment length polymorphisms in the rat N-ras and retinoblastoma genes: their identification and characterization.
We have observed restriction fragment length polymorphisms for the N-ras and retinoblastoma (Rb) genes between rat strains that are susceptible or resistant to induction of pituitary tumors by diethylstilbestrol (DES). Thirteen other proto-oncogenes tested displayed the same restriction patterns between all samples. The N-ras polymorphism is observed with more than one restriction nuclease and the N-ras and Rb polymorphisms are not a result of DES treatment. The N-ras polymorphism is inherited in a Mendelian fashion and there appear to be two separate loci in the rat genome that are detected by the N-ras probe. For both the N-ras and Rb genes there is an 'extra' fragment in the tumor-resistant animals that is not seen in the susceptible rats. These polymorphisms may indicate regions of the genome that play a role in determining susceptibility to pituitary tumors or any of a number of other chemically induced tumors.
Shepel
L A
LA
Department of Biochemistry, University of Wisconsin-Madison 53706.
Gorski
J
J
D000818:Animals; D003433:Crosses, Genetic; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D004054:Diethylstilbestrol; D005260:Female; D011905:Genes, ras; D008297:Male; D009693:Nucleic Acid Hybridization; D010911:Pituitary Neoplasms / Q000139:chemically induced / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains; D012175:Retinoblastoma / Q000235:genetics*
D004054:Diethylstilbestrol; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific