title: Effect of calcium-modifying drugs on mouse in vitro fertilization and preimplantation development.
abstract: Calcium ions are required for normal in vitro fertilization and preimplantation development in the mouse. This study examined the effects of alterations in Ca2+ flux and distribution on sperm penetration of eggs and embryo cleavage. Compounds used included diltiazem, a Ca2+ channel blocker, and 8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate (TMB-8), an antagonist of intracellular calcium release. Incubation of sperm and eggs with diltiazem at 30 and 10 microM and TMB-8 at 30, 10, and 3 microM resulted in depressed fertilization compared with controls. Motility was not inhibited at these concentrations of either drug. Both drugs also depressed progression of mouse embryos from 2-cell to blastocyst at 30 and 3-microM concentrations. This study suggests that both Ca2+ flux and distribution to specific cellular sites are required for normal mouse in vitro fertilization and early preimplantation development.
authors.affiliation: Department of Obstetrics and Gynecology, Georgetown University Medical School, Washington, D.C.
authors.forename: J K
authors.initials: JK
authors.lastname: Blancato
authors.forename: D E
authors.initials: DE
authors.lastname: Seyler
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000187:drug effects*; D002118:Calcium / Q000502:physiology; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D004110:Diltiazem / Q000494:pharmacology*; D005260:Female; D005307:Fertilization in Vitro / Q000187:drug effects*; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013081:Sperm Motility / Q000187:drug effects / Q000502:physiology; D013084:Sperm-Ovum Interactions / Q000187:drug effects / Q000502:physiology
chemical_list: D002121:Calcium Channel Blockers; C006014:8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate; D005707:Gallic Acid; D004110:Diltiazem; D002118:Calcium