[3H]Dihydrotetrabenazine, a new marker for the visualization of dopaminergic denervation in the rat striatum.
[3H]Dihydrotetrabenazine ([3H]TBZOH), a derivative of the monoamine depleting agent, tetrabenazine, has been shown to bind to the vesicular monoamine transporter. We studied the effect of unilateral nigral lesion with 6-hydroxydopamine on [3H]TBZOH binding in the striatum by means of quantitative autoradiography. Topographical analysis of striatal [3H]TBZOH binding showed in control rats a pattern similar to the known distribution of dopaminergic innervation, and allowed to visualize dopaminergic denervation in lesioned rats. A good correlation was found between striatal [3H]TBZOH binding levels and tyrosine hydroxylase activities in similar areas on adjacent slices following 6-OHDA lesions with varying severity. These data reveal that autoradiography of [3H]TBZOH binding is a good index for the visualization and quantitation of dopaminergic nerve terminals integrity in the striatum.
Masuo
Y
Y
INSERM U.55, Hôpital Saint-Antoine, Paris, France.
Pélaprat
D
D
Scherman
D
D
Rostène
W
W
D000818:Animals; D001345:Autoradiography; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D003714:Denervation; D004298:Dopamine / Q000502:physiology*; D006892:Hydroxydopamines / Q000633:toxicity; D007700:Kinetics; D008297:Male; D016627:Oxidopamine; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D013378:Substantia Nigra / Q000502:physiology; D013747:Tetrabenazine / Q000031:analogs & derivatives* / Q000378:metabolism; D014316:Tritium; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D006892:Hydroxydopamines; D014316:Tritium; C032811:dihydrotetrabenazine; D016627:Oxidopamine; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine; D013747:Tetrabenazine