Production by cultured human monocytes of mesangial cell proliferation factor(s) differing from interleukin-1 and interleukin-6.
Conditioned media from human peripheral blood leucocytes treated with lipopolysaccharide (LPS) induced a marked increase in the 3H-thymidine incorporation of cultured mesangial cells at low serum concentration (four to six times higher than control). Two sizes (100-70 and 8-12 kD) of monocyte-derived mesangial cell proliferating factors (MDF) were separated by column chromatography. Their peaks were distinct from those of thymocyte proliferating activity. The addition of anti-human interleukin-1 (IL-1) or anti-recombinant human interleukin-6 (IL-6) antibody to the fractionated MDF failed to have any effect on the mitogenic activity toward mesangial cells. The addition of anti-human platelet-derived growth factor (PDGF) antibody to the low molecular weight fraction decreased mesangial cell mitogenic activity (40-60% of control), but addition to the higher fraction did not (80-100% of control). From these data it seems that a large portion of the monocyte-derived mesangial cell growth factor was not comprised of IL-1 or IL-6 but of PDGF-like molecules; and that there is an unknown mesangial cell proliferating factor (or factors) besides IL-1, IL-6 and PDGF.
Morioka
T
T
Department of Immunology, Niigata University School of Medicine, Japan.
Narita
I
I
Shimizu
F
F
Oite
T
T
D000328:Adult; D000906:Antibodies; D002455:Cell Division; D002478:Cells, Cultured; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005920:Glomerular Mesangium / Q000166:cytology*; D006133:Growth Substances / Q000096:biosynthesis* / Q000302:isolation & purification; D006801:Humans; D007375:Interleukin-1 / Q000276:immunology / Q000502:physiology*; D015850:Interleukin-6 / Q000276:immunology / Q000502:physiology*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008938:Mitosis; D010982:Platelet-Derived Growth Factor / Q000276:immunology / Q000502:physiology; D011994:Recombinant Proteins; D013950:Thymus Gland / Q000166:cytology
D000906:Antibodies; D006133:Growth Substances; D007375:Interleukin-1; D015850:Interleukin-6; D010982:Platelet-Derived Growth Factor; D011994:Recombinant Proteins