title: Retention of the mitochondrial probe rhodamine 123 in normal lymphocytes and leukemic cells in relation to the cell cycle.
abstract: The cationic fluorochrome rhodamine 123 (R123) is specifically taken up by mitochondria of live cells where it is retained due to the mitochondrial transmembrane potential. After pulse exposure of human normal quiescent or proliferating lymphocytes, human lymphocytic leukemic MOLT cells, and mice leukemic L1210 cells to 10 micrograms/ml of R123, the dye release was studied using flow cytometry. Two distinct phases of R123 release, each following first-order kinetics, were apparent; the half-time of retention for the rapidly and slowly released fractions of R123 was 0.8-1.1 and 2.8-4.2 h, respectively. Simultaneous supravital cell staining with R123 and Hoechst 33342 made it possible to correlate retention of R123 with cell position in the cell cycle. No significant differences were observed in the rate of R123 release from cells in G1 vs S or vs G2 + M phases of the cycle. The data rule out a possibility that the release of R123 is due to periodic depolarization of the mitochondria in the cell as may be postulated by cell cycle models that assume a transient passage of cells through resting phase following division. The observed similar rates of R123 release regardless of cell type or cell cycle phase suggest that the factors affecting the exchange are similar in normal lymphocytes vs leukemic cells and unrelated to cell proliferation rate or phase of the cell cycle. Two distinct rates of R123 release indicate the presence of two kinds of binding sites differing in affinity to the dye.
authors.affiliation: Sloan-Kettering Institute for Cancer Research, New York, New York 10021.
authors.forename: A
authors.initials: A
authors.lastname: Myc
authors.forename: P
authors.initials: P
authors.lastname: DeAngelis
authors.forename: M
authors.initials: M
authors.lastname: Kimmel
authors.forename: M R
authors.initials: MR
authors.lastname: Melamed
authors.forename: Z
authors.initials: Z
authors.lastname: Darzynkiewicz
mesh_terms: D002453:Cell Cycle / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D005456:Fluorescent Dyes / Q000378:metabolism*; D006801:Humans; D007945:Leukemia, Lymphoid / Q000378:metabolism*; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D020112:Rhodamine 123; D012235:Rhodamines / Q000378:metabolism*; D013997:Time Factors; D014407:Tumor Cells, Cultured
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; D020112:Rhodamine 123