Somatostatin content and binding in small intestinal mucosa from fed, fasted, and refed rabbits.
The present study is an investigation of the effects of 12- to 96-hours' starvation and 96-hours' starvation plus 48-hours' refeeding on both somatostatin-like immunoreactivity (SLI) and cytosolic somatostatin binding sites in rabbit small intestinal mucosa. The SLI concentration increased after 24 h in duodenal and jejunal mucosa, but not in ileal mucosa, and reached its highest value after 96 h of fasting. The number of specific high and low-affinity somatostatin binding sites, but not their affinity, decreased with the duration of fasting in the same gut segments, refeeding of fasted animals resulted in a return to normal control values for small intestine mucosal SLI and somatostatin binding.
Roca
B
B
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Alcalá de Henares, Madrid, Spain.
Fernandez-Valencia
R
R
Rodríguez-Sánchez
M N
MN
Arilla
E
E
D000818:Animals; D001667:Binding, Competitive; D004386:Duodenum / Q000378:metabolism; D005215:Fasting; D005502:Food; D007082:Ileum / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D007421:Intestine, Small / Q000378:metabolism*; D007457:Iodine Radioisotopes; D007583:Jejunum / Q000378:metabolism; D008297:Male; D011817:Rabbits; D013004:Somatostatin / Q000031:analogs & derivatives / Q000378:metabolism*
D007457:Iodine Radioisotopes; D013004:Somatostatin; C032367:somatostatin, Tyr(11)-