title: Reduction of transmural 125I-albumin concentration in rat aortic media by chronic hypertension.
abstract: Relative 125I-albumin concentration was measured in vivo in the aortic media of sham-operated (n = 10) and hypertensive (two-kidney, one clip) rats, untreated (n = 8) or treated (n = 10) by an angiotensin converting enzyme inhibitor (CEI, Trandolapril). Blood pressure was acutely lowered to a normal level at the time of the experiment in hypertensive rats (n = 7) to separate the direct effect of increased pressure from the effect of pressure-induced structural changes. Relative tissue concentration profiles of labeled albumin across the media were obtained using a serial frozen-sectioning technique. In hypertensive rats, the mean medial albumin concentration decreased by 35% in the ascending arch and 32% in the descending arch (p less than 0.01). When blood pressure was acutely lowered in hypertensive animals, this value decreased further by 56% in the ascending arch, 48% in the descending arch (p less than 0.01), and 22% in the thoracic aorta (p less than 0.05) as compared with controls. The medial thickness in hypertensive rats was significantly increased (more in the ascending arch than in the rest of the aorta). Four-week CEI treatment reversed hypertension and medial thickening, but the mean medial albumin concentration remained significantly lower in the arch (by 36% in the ascending part and 40% in the descending part, p less than 0.01). The collagen content in the thoracic aorta was significantly increased in hypertensive rats (by 40%, p less than 0.01) and remained increased (by 29%, p less than 0.01) after CEI treatment. These results suggested that the hypertension-induced structural changes might reduce the medial distribution volume for albumin, whereas elevated blood pressure per se tended to enhance albumin concentration within the media. However, the net result of chronic hypertension was a reduction of the mean medial albumin concentration. The aortic arch appeared to be more affected than the rest of the aorta. Fiber content, more than medial thickness, might be responsible for the observed differences in albumin concentration. Lowering of blood pressure seemed to be insufficient to restore normal albumin concentration profiles and perhaps those of other macromolecules. This finding may be relevant in evaluating some of the complications associated with hypertension.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Unité 141, Hôpital Lariboisière, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Belmin
authors.forename: J B
authors.initials: JB
authors.lastname: Michel
authors.forename: P A
authors.initials: PA
authors.lastname: Curmi
authors.forename: J L
authors.initials: JL
authors.lastname: Salzmann
authors.forename: L
authors.initials: L
authors.lastname: Juan
authors.forename: A
authors.initials: A
authors.lastname: Tedgui
mesh_terms: D000418:Albumins / Q000493:pharmacokinetics*; D000818:Animals; D001011:Aorta / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000473:pathology; D001012:Aorta, Abdominal / Q000378:metabolism / Q000473:pathology; D001013:Aorta, Thoracic / Q000378:metabolism / Q000473:pathology; D001692:Biological Transport / Q000502:physiology; D002908:Chronic Disease; D003094:Collagen / Q000032:analysis; D006973:Hypertension / Q000378:metabolism* / Q000473:pathology; D007457:Iodine Radioisotopes; D008297:Male; D011877:Radionuclide Imaging; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
chemical_list: D000418:Albumins; D007457:Iodine Radioisotopes; D003094:Collagen