Detection of D,L-amphetamine, D,L-methamphetamine, and illicit amphetamine analogs using diagnostic products corporation's amphetamine and methamphetamine radioimmunoassay.
Cross-reactivity with Diagnostic Products Corporation (DPC) amphetamine and methamphetamine radioimmunoassay (RIA) reagents was determined for amphetamine, methamphetamine, and a number of amphetamine analogs. Concentrations from 100 to 100,000 ng/mL were assayed. 3,4-Methylenedioxyamphetamine (MDA) and 3,4-methylenedioxymethamphetamine (MDMA) showed significant cross-reactivity for the amphetamine and methamphetamine reagents respectively. 4-Hydroxymethamphetamine, 3,4-methylenedioxyethylamphetamine (MDEA), and N,N-dimethyl-MDA also showed significant cross-reactivity with the methamphetamine reagents, but less than MDMA. None of the other analogs showed a positive result with the amphetamine or methamphetamine reagents at even the highest concentration, although several did show measurable cross-reactivity. The L isomers of amphetamine and methamphetamine showed substantially less cross-reactivity than the D forms to which the respective antibody systems are targeted.
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Air Force Drug Testing Laboratory, Brooks AFB, Texas 78235-5000.
D015104:3,4-Methylenedioxyamphetamine / Q000031:analogs & derivatives / Q000032:analysis / Q000276:immunology; D000662:Amphetamines / Q000032:analysis*; D003429:Cross Reactions; D007202:Indicators and Reagents; D007536:Isomerism; D008694:Methamphetamine / Q000032:analysis*; D018817:N-Methyl-3,4-methylenedioxyamphetamine; D011863:Radioimmunoassay
D000662:Amphetamines; D007202:Indicators and Reagents; D008694:Methamphetamine; D015104:3,4-Methylenedioxyamphetamine; D018817:N-Methyl-3,4-methylenedioxyamphetamine