Processing and intracellular sorting of anglerfish and rat preprosomatostatins in mammalian endocrine cells.
Rat preprosomatostatin (rPPSS) is processed to two distinct end products in a tissue-specific manner. The analogous end products in anglerfish are derived from separate precursors, anglerfish preprosomatostatins-1 and -2 (a(1)PPSS and a(II)PPSS). This report reviews experiments demonstrating that in mammalian cells, the cell of expression, not precursor structure, determines the processing fate of the preprosomatostatins. A fusion precursor of a(II)PPSS and rPPSS was expressed in mammalian cell lines to determine that the amino-terminal 78 residues of rPPSS contain a sorting signal that directs the precursor into a regulated secretory pathway wherein proteolytic processing occurs. Preliminary studies of rPPSS pro-region mutations are presented that attempt to further localize this sorting signal.
Sevarino
K A
KA
Department of Psychiatry, Yale University School of Medicine, New Haven, CT 06508.
Ventimiglia
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R
Stork
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P
D000818:Animals; D003001:Cloning, Molecular; D005399:Fishes; D009154:Mutation; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D013004:Somatostatin / Q000235:genetics* / Q000378:metabolism; D013045:Species Specificity
D011498:Protein Precursors; D013004:Somatostatin