title: Allele- and haploid-specific product generated by alternative splicing from a mouse t complex responder locus candidate.
abstract: Mouse t haplotypes represent a variant form of chromosome 17 that has evolved the ability to propagate through natural populations by the phenomenon of 'transmission ratio distortion' (TRD), in which heterozygous +/t males transmit their t-carrying chromosome to 95% or more of their offspring. Although multiple t-associated loci have a role in expression of this phenotype, only one--the t complex responder (Tcr) locus--is responsible for determining which of the two homologues of chromosome 17 will be transmitted at a high ratio. A candidate gene (Tcp-10b) for Tcr that is expressed in both meiotic and post-meiotic male germ cells has been cloned. But for this candidate gene to function as the haploid effector of TRD, the t-allele of this gene (Tcp-10bt) must express a unique product in a haploid-specific manner. Here we show that a change in the splicing pattern of Tcp-10bt transcripts occurs during sperm differentiation. This change results in a unique allele-specific and haploid-specific transcript which could encode a variant polypeptide that would fulfil the conditions required of the Tcr effector of TRD.
authors.affiliation: Department of Molecular Biology, Princeton University, New Jersey 08544-1014.
authors.forename: J A
authors.initials: JA
authors.lastname: Cebra-Thomas
authors.forename: C L
authors.initials: CL
authors.lastname: Decker
authors.forename: L C
authors.initials: LC
authors.lastname: Snyder
authors.forename: S H
authors.initials: SH
authors.lastname: Pilder
authors.forename: L M
authors.initials: LM
authors.lastname: Silver
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002875:Chromosomes / Q000502:physiology*; D005786:Gene Expression Regulation / Q000235:genetics; D005809:Genes, Regulator / Q000235:genetics*; D006239:Haplotypes; D008297:Male; D008540:Meiosis / Q000502:physiology; D051379:Mice; D008938:Mitosis / Q000502:physiology; D008969:Molecular Sequence Data; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D012333:RNA, Messenger / Q000032:analysis; D013091:Spermatogenesis / Q000235:genetics
chemical_list: D012333:RNA, Messenger