title: Human dopamine D1 receptor encoded by an intronless gene on chromosome 5.
abstract: Receptors for dopamine have been classified into two functional types, D1 and D2. They belong to the family of receptors acting through G (or guanine nucleotide-binding) proteins. D2 receptors inhibit adenylyl cyclase, but D1 receptors stimulate adenylyl cyclase and activate cyclic AMP-dependent protein kinases. Dopamine D1 and D2 receptors are targets of drug therapy in many psychomotor disorders, including Parkinson's disease and schizophrenia, and may also have a role in drug addiction and alcoholism. D1 receptors regulate neuron growth and differentiation, influence behaviour and modify dopamine D2 receptor-mediated events. We report here the cloning of the D1 receptor gene, which resides on an intronless region on the long arm of chromosome 5, near two other members of the G-linked receptor family. The expressed protein, encoded by 446 amino acids, binds drugs with affinities identical to the native human D1 receptor. The presence of a D1 receptor gene restriction fragment length polymorphism will be helpful for future disease linkage studies.
authors.affiliation: Department of Pharmacology, University of Toronto, Ontario, Canada.
authors.forename: R K
authors.initials: RK
authors.lastname: Sunahara
authors.forename: H B
authors.initials: HB
authors.lastname: Niznik
authors.forename: D M
authors.initials: DM
authors.lastname: Weiner
authors.forename: T M
authors.initials: TM
authors.lastname: Stormann
authors.forename: M R
authors.initials: MR
authors.lastname: Brann
authors.forename: J L
authors.initials: JL
authors.lastname: Kennedy
authors.forename: J E
authors.initials: JE
authors.lastname: Gelernter
authors.forename: R
authors.initials: R
authors.lastname: Rozmahel
authors.forename: Y L
authors.initials: YL
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Israel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001552:Benzazepines / Q000378:metabolism; D001923:Brain Chemistry; D002417:Cattle; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular; D006031:Glycosylation; D006801:Humans; D007438:Introns; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010766:Phosphorylation; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D011954:Receptors, Dopamine / Q000235:genetics*; D017447:Receptors, Dopamine D1; D015183:Restriction Mapping; D014018:Tissue Distribution; D014162:Transfection
chemical_list: D001552:Benzazepines; D012333:RNA, Messenger; D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1