title: Evaluation of practical chromatographic procedures for identification of clinical isolates of mycobacteria.
abstract: After experimental conditions were established, 366 strains of mycobacteria belonging to 23 different species were studied for fatty acids, secondary alcohols, and mycolic acid cleavage products by capillary gas-liquid chromatography. Additionally, the mycolic acid pattern was studied by thin-layer chromatography. Capillary gas-liquid chromatography allowed direct identification of the following Mycobacterium spp.: M. kansasii, M. marinum, M. szulgai, M. xenopi, M. malmoense, and M. gordonae. The patterns of mycolic acid methyl esters recorded for the test strains of M. chelonae and M. agri may be of value in the identification of these species. Moreover, the combined use of the two chromatographic techniques provided precise identification of the M. tuberculosis complex, M. simiae, M. fallax, M. triviale, and M. chelonae-like organisms. A minimal set of biochemical tests is usually required to obtain identification to the species level when chromatographic procedures alone are not sufficient. Under the reported experimental conditions, thin-layer chromatography and capillary gas-liquid chromatography are rapid and very useful techniques for the identification of mycobacteria.
authors.affiliation: Departamento de Microbiología, Hospital de la Santa Cruz y San Pablo, Facultad de Medicina de la Universidad Autónoma de Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Luquin
authors.forename: V
authors.initials: V
authors.lastname: Ausina
authors.forename: F
authors.initials: F
authors.lastname: López Calahorra
authors.forename: F
authors.initials: F
authors.lastname: Belda
authors.forename: M
authors.initials: M
authors.lastname: García Barceló
authors.forename: C
authors.initials: C
authors.lastname: Celma
authors.forename: G
authors.initials: G
authors.lastname: Prats
mesh_terms: D000438:Alcohols / Q000032:analysis; D002849:Chromatography, Gas / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods; D005069:Evaluation Studies as Topic; D005227:Fatty Acids / Q000032:analysis; D009161:Mycobacterium / Q000145:classification* / Q000254:growth & development; D009171:Mycolic Acids / Q000032:analysis; D015203:Reproducibility of Results; D013045:Species Specificity
chemical_list: D000438:Alcohols; D005227:Fatty Acids; D009171:Mycolic Acids