Quantitative and qualitative analysis of DNA extracted from postmortem muscle tissues.
DNA extracted from 33 postmortem muscle specimens was analyzed using MZ 1.3, a hypervariable minisatellite probe, as well as locus-specific minisatellite probes (g3, MS1 and MS43). After storage at -25 degrees C for 10 months, DNA from all the samples was partially (approximately 21% of total DNA) degraded even when autopsy was performed 1 day postmortem. However, more than 90% of DNA samples up to at least 3 days postmortem were suitable to obtain good restriction fragment length polymorphism (RFLP) patterns. When small strips of specimen were stored for 8 days at room temperature in moist chambers, approximately 42% of total DNA was degraded. Only 30% of these DNA samples still showed good RFLP patterns. However, no obvious relation between qualities of DNA analyzed by detection of RFLP and quantities of total and high-MW DNA became apparent. A case of familial relationship was ascertained by DNA fingerprints. Since DNA of good quality can be recovered from muscle tissues in large quantities, DNA extraction from muscle tissues and detection of RFLP patterns should be very useful for individual identification in autopsy cases.
Ogata
M
M
Institute of Legal Medicine, Johannes Gutenberg University, Mainz, Federal Republic of Germany.
Mattern
R
R
Schneider
P M
PM
Schacker
U
U
Kaufmann
T
T
Rittner
C
C
D000060:Accidents, Aviation / Q000331:legislation & jurisprudence; D000293:Adolescent; D000328:Adult; D000368:Aged; D002056:Burns / Q000473:pathology; D002648:Child; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D004276:DNA, Satellite / Q000235:genetics; D003645:Death, Sudden / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D009104:Multiple Trauma / Q000473:pathology; D009132:Muscles / Q000473:pathology*; D012150:Polymorphism, Restriction Fragment Length; D011180:Postmortem Changes; D013997:Time Factors
D015342:DNA Probes; D004276:DNA, Satellite; D004247:DNA