Identification of a new V kappa gene family that is highly expressed in hybridomas from an autoimmune mouse strain.
We have identified a new murine V kappa family that contains five to seven members, one member of which encodes the L chain V region of an anti-dsDNA antibody produced by a BALB/c hybridoma, C8.5. The cloned C8.5 V kappa gene exhibits highest homology with a human V kappa gene that was cloned from a nonproductive rearrangement but has never been seen in an expressed repertoire. Because this family was first identified in an autoantibody, we studied its expression in an autoimmune mouse strain. This V kappa family is expressed in 20% of hybridomas from NZB mice.
Shefner
R
R
Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461.
Mayer
R
R
Kaushik
A
A
D'Eustachio
P
P
Bona
C
C
Diamond
B
B
D000595:Amino Acid Sequence; D000818:Animals; D001327:Autoimmune Diseases / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D015870:Gene Expression; D005803:Genes, Immunoglobulin; D006825:Hybridomas; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D005810:Multigene Family; D009841:Oligonucleotides; D012150:Polymorphism, Restriction Fragment Length; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping
D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains; D009841:Oligonucleotides; D012333:RNA, Messenger