Macrophage colony-stimulating factor activity in malignant pleural effusions. Its augmentation by intrapleural interleukin-2 infusions.
The activity of endogenous colony-stimulating factor (CSF) in malignant pleural effusions of lung cancer patients before and during daily intrapleural infusions of recombinant interleukin-2 (IL-2) was measured quantitatively by colony-forming bioassay and radioimmunoassay (RIA). Before therapy, malignant pleural effusions had various levels of CSF activities, and this CSF activity was neutralized almost completely by anti-M-CSF antibody. RIA also showed that the effusions contained various amounts of M-CSF. Daily intrapleural infusion of recombinant IL-2 caused significant increase in the CSF activities and M-CSF levels in pleural effusions. These results indicate that in vivo treatment with IL-2 induces production of endogenous M-CSF.
Sone
S
S
Third Department of Internal Medicine, University of Tokushima, School of Medicine, Japan.
Nakanishi
M
M
Ohmoto
Y
Y
Yanagawa
H
H
Ogura
T
T
D000328:Adult; D000368:Aged; D003114:Colony-Forming Units Assay; D006801:Humans; D007376:Interleukin-2 / Q000627:therapeutic use*; D008175:Lung Neoplasms / Q000150:complications / Q000378:metabolism / Q000628:therapy; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D008875:Middle Aged; D016066:Pleural Effusion, Malignant / Q000209:etiology / Q000378:metabolism*; D011863:Radioimmunoassay; D011994:Recombinant Proteins
D007376:Interleukin-2; D011994:Recombinant Proteins; D016173:Macrophage Colony-Stimulating Factor