Proteins of bovine ephemeral fever virus.
The proteins of bovine ephemeral fever virus (BEFV) were examined in purified virions and in infected BHK-21 cells. Five structural proteins were named L (180K), G (81K), N (52K), M1 (43K) and M2 (29K). The 81K G protein incorporated [3H]glucosamine, was removed from virions by treatment with Triton X-100 and bound monoclonal antibodies which were both neutralizing and protective. Treatment of virions with Triton X-100 and 0.2 to 1.0 M-NaCl progressively released L, M1 and M2. The N protein remained associated with nucleocapsids in up to 2.5 M-NaCl. The glycoprotein (G), nucleoprotein (N) and matrix protein (M2) were phosphorylated. In BEFV-infected BHK-21 cells, five virus-induced proteins were detected from 12 h post-infection. The L, N, M1 and M2 proteins corresponded to those detected in virions whereas the G protein existed in two forms. In tunicamycin-treated cells these occurred as 67K and 71K non-glycosylated precursors. In the absence of tunicamycin, 77K and 79K glycosylated forms were further modified to produce the 81K virion G protein and a 90K cell-associated form. Five viral proteins were also detected in cells infected with the closely related Berrimah virus; the Berrimah virus G protein was also present in two forms.
Walker
P J
PJ
CSIRO, Division of Tropical Animal Production, Indooroopilly, Queensland, Australia.
Byrne
K A
KA
Cybinski
D H
DH
Doolan
D L
DL
Wang
Y H
YH
D000818:Animals; D002417:Cattle; D002460:Cell Line; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D004810:Ephemeral Fever / Q000382:microbiology*; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational; D012328:RNA Viruses / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D015678:Viral Structural Proteins / Q000302:isolation & purification; D014771:Virion / Q000302:isolation & purification
D003902:Detergents; D014764:Viral Proteins; D015678:Viral Structural Proteins