FPLC purification of mouse monoclonal antibodies from ascitic fluid using blue DEAE and thiophilic sorbents.
Two fast methods for the purification of mouse monoclonal antibodies from ascites, fluids using Blue-DEAE and 'thiophilic' adsorbent (T gel) in the FPLC system are described. Blue-DEAE chromatography is useful only for IgG1, IgG2a and IgG2b monoclonal antibody purification. T gel is a satisfactory adsorbent for IgG2b purification. Other IgG subclasses and IgM can also be obtained by simple one-step procedures, but the preparations contain small amount of high molecular weight contaminants.
Juronen
E
E
Institute of General and Molecular Pathology, Tartu University, Estonia, U.S.S.R.
Parik
J
J
Toomik
P
P
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D001202:Ascitic Fluid / Q000276:immunology*; D002853:Chromatography, Liquid; D004983:Ethanolamines; D005782:Gels; D007074:Immunoglobulin G / Q000302:isolation & purification*; D051379:Mice
D000911:Antibodies, Monoclonal; D004983:Ethanolamines; D005782:Gels; D007074:Immunoglobulin G; C007369:2-diethylaminoethanol