The human interleukin-2 receptor beta-chain gene: genomic organization, promoter analysis and chromosomal assignment.
The chromosomal gene for the human interleukin-2 receptor beta-chain (IL-2R beta) was isolated and characterized. The entire IL-2R beta gene is composed of ten exons spanning about 24.3 kilobases, in which the protein is encoded by the exons 2-10. The cysteine rich extracellular region which displays a significant evolutionary resemblance to other cytokine receptors, as well as growth hormone and prolactin receptors, is encoded primarily by exons 3 and 4, whereas the membrane proximal, cysteine poor domain showing a homology with type III modules of fibronectin is encoded by exon 7. Sequence analysis of the 5'-flanking region revealed the presence of potential binding sites for transcription factors such as Octamer binding factors, AP-1, AP-2 as well as the 'GC-clusters'. At least five potential cap sites were identified by S1 mapping analysis. The 850 bp DNA sequence of the 5'-flanking region exhibited constitutive promoter activity when it was linked upstream of the HSV-tk reporter gene and then transfected into YT cells, a human leukemic cell line. By applying the RFLP linkage analysis, the IL-2R beta gene has been assigned to chromosome 22q12-13.
Shibuya
H
H
Institute for Molecular and Cellular Biology, Osaka University, Japan.
Yoneyama
M
M
Nakamura
Y
Y
Harada
H
H
Hatakeyama
M
M
Minamoto
S
S
Kono
T
T
Doi
T
T
White
R
R
Taniguchi
T
T
D001483:Base Sequence; D001665:Binding Sites; D005075:Biological Evolution; D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22; D004268:DNA-Binding Proteins / Q000378:metabolism; D005091:Exons; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic; D016755:Proto-Oncogene Proteins c-jun; D015375:Receptors, Interleukin-2 / Q000235:genetics*; D017981:Receptors, Neurotransmitter / Q000235:genetics; D011981:Receptors, Prolactin / Q000235:genetics; D017481:Receptors, Somatostatin; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D050656:Transcription Factor AP-2; D014157:Transcription Factors / Q000378:metabolism
D004268:DNA-Binding Proteins; D016755:Proto-Oncogene Proteins c-jun; D015375:Receptors, Interleukin-2; D017981:Receptors, Neurotransmitter; D011981:Receptors, Prolactin; D017481:Receptors, Somatostatin; D050656:Transcription Factor AP-2; D014157:Transcription Factors