Identification of asialo GM1 as a binding structure for Escherichia coli colonization factor antigens.
We have examined the binding of colonization factor antigens, (CFAs) of enterotoxigenic Escherichia coli to gangliosides and asialo gangliosides by using immuno-thin layer chromatography. CFA/II and its subcomponents (CS1, CS2 and CS3) as well as the subcomponent CS4 of the CFA/IV complex bound to asialo ganglioside GM1.
Orø
H S
HS
Biotechnology Centre, Oslo, Norway.
Kolstø
A B
AB
Wennerås
C
C
Svennerholm
A M
AM
D000942:Antigens, Bacterial / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D004926:Escherichia coli / Q000276:immunology; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000276:immunology; D005677:G(M1) Ganglioside; D006017:Glycolipids / Q000276:immunology*; D006028:Glycosphingolipids / Q000378:metabolism*; D007118:Immunoassay
D000942:Antigens, Bacterial; D006017:Glycolipids; D006028:Glycosphingolipids; C022924:colonization factor antigens; D036781:Fimbriae Proteins; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside