Novel cell lines display properties of nociceptive sensory neurons.
Hybrid cell lines derived from neonatal rat dorsal root ganglia neurons fused with the mouse neuroblastoma N18Tg2 exhibit sensory neuron-like properties not displayed by the parental neuroblastoma. These properties include an inward (depolarizing) current with a conductance increase in response to activation of a bradykinin receptor, an inward (depolarizing) current with a conductance increase in response to the sensory excitotoxin capsaicin, the expression of sensory neuropeptides (substance P, CGRP and somatostatin), the expression of phosphatidylinositol-anchored molecules including adhesion molecules of the immunoglobulin superfamily that can be regulated in serum-free culture by nerve growth factor (N-CAM, F-3 and Thy-1), and low permissivity to herpes simplex virus infection. These lines thus provide appropriate models for the study of mechanisms involved in nociceptor activation and the regulation of expression of sensory-neuron specific markers including neuropeptides.
Wood
J N
JN
Sandoz Institute for Medical Research, London, U.K.
Bevan
S J
SJ
Coote
P R
PR
Dunn
P M
PM
Harmar
A
A
Hogan
P
P
Latchman
D S
DS
Morrison
C
C
Rougon
G
G
Theveniau
M
M
D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D002211:Capsaicin / Q000494:pharmacology; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis; D002454:Cell Differentiation; D002459:Cell Fusion; D002460:Cell Line; D002472:Cell Transformation, Viral; D004553:Electric Conductivity; D005727:Ganglia, Spinal / Q000502:physiology; D006822:Hybrid Cells / Q000166:cytology / Q000502:physiology*; D051379:Mice; D009447:Neuroblastoma; D009475:Neurons, Afferent / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009479:Neuropeptides / Q000494:pharmacology; D009619:Nociceptors / Q000502:physiology*; D018002:Receptors, Bradykinin; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000502:physiology; D018139:Simplexvirus / Q000235:genetics
D015816:Cell Adhesion Molecules, Neuronal; D009479:Neuropeptides; D018002:Receptors, Bradykinin; D017981:Receptors, Neurotransmitter; D002211:Capsaicin; D001920:Bradykinin