title: Homology of melanoma-inducing loci in the genus Xiphophorus.
abstract: Several species of the genus Xiphophorus are polymorphic for specific pigment patterns. Some of these give rise to malignant melanoma following the appropriate crossings. For one of these pattern loci from the playfish Xiphophorus maculatus the melanoma-inducing gene has been cloned and found to encode a novel receptor tyrosine kinase, designated Xmrk. Using molecular probes from this gene in Southern blot analyses on single fish DNA preparations from 600 specimens of different populations of various species of the genus Xiphophorus and their hybrids, either with or without melanoma-predisposing pattern, it was shown that all individuals contain the Xmrk gene as a proto-oncogene. It is located on the sex chromosome. All fish that carry a melanoma-predisposing locus which has been identified by Mendelian genetics contain an additional copy of Xmrk, closely linked to a specific melanophore pattern locus on the sex chromosome. The melanoma-inducing loci of the different species and populations are homologous. The additional copy of Xmrk obviously arose by a gene-duplication event, thereby acquiring the oncogenic potential. The homology of the melanoma-inducing loci points to a similar mechanism of tumor suppression in all feral fish populations of the different species of the genus Xiphophorus.
authors.affiliation: Genzentrum/Max-Planck Institut für Biochemie, Martinsried, Federal Republic of Germany.
authors.forename: M
authors.initials: M
authors.lastname: Schartl
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D003532:Cyprinodontiformes / Q000235:genetics*; D005260:Female; D005393:Fish Diseases / Q000235:genetics; D008040:Genetic Linkage; D008297:Male; D008545:Melanoma / Q000235:genetics* / Q000662:veterinary; D009857:Oncogenes; D012150:Polymorphism, Restriction Fragment Length; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011519:Proto-Oncogenes; D011956:Receptors, Cell Surface / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D011956:Receptors, Cell Surface; D011505:Protein-Tyrosine Kinases