title: Protein kinase C inhibitors prevent induction and continued expression of cell memory in Hermissenda type B photoreceptors.
abstract: Injections of cAMP-dependent, Ca2+/calmodulin-dependent, or Ca2+/phospholipid-dependent protein kinases into Hermissenda crassicornis type B photoreceptors are sufficient to induce many of the changes in B-cell excitability produced by associative conditioning. We report that inhibitors of Ca2+/phospholipid-dependent protein kinases, but not inhibitors of cyclic nucleotide- or Ca2+/calmodulin-dependent protein kinases, prevent the induction as well as continued expression of learning-produced changes in type-B-cell excitability: reductions of voltage-dependent and Ca2(+)-activated K+ currents. Our results represent a direct demonstration of long-term (days) experientially induced modulation of ion-channel activity that is dependent upon persistent kinase activity.
authors.affiliation: Program in Neural Science, Indiana University, Bloomington 47405.
authors.forename: J
authors.initials: J
authors.lastname: Farley
authors.forename: E
authors.initials: E
authors.lastname: Schuman
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D004558:Electric Stimulation; D066298:In Vitro Techniques; D007546:Isoquinolines / Q000494:pharmacology*; D008839:Microelectrodes; D009424:Nervous System Physiological Phenomena; D010786:Photoreceptor Cells / Q000187:drug effects / Q000502:physiology*; D010879:Piperazines / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D012908:Snails / Q000502:physiology*; D013110:Sphingosine / Q000494:pharmacology*; D019311:Staurosporine
chemical_list: D000470:Alkaloids; D007546:Isoquinolines; D010879:Piperazines; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011493:Protein Kinase C; D019311:Staurosporine; D013110:Sphingosine; D002118:Calcium