Modulation of melanocyte intercellular adhesion molecule-1 by immune cytokines.
Human melanocyte expression of intercellular adhesion molecule-1 (ICAM-1) with or without stimulation by interferon gamma (IFN-G), tumor necrosis factor alpha (TNF-alpha), or interleukin-1-alpha (IL-1 alpha), was measured utilizing direct immunofluorescence and fluorescence-activated cell sorting (FACS). Melanocytes grown in vitro expressed low levels of ICAM-1, which could be increased by exposing the cells to IFN-G, TNF-alpha, or IL-1 alpha. Each cytokine caused an enhancement of melanocyte ICAM-1 expression in a dose-dependent fashion. The lowest dose necessary to cause a significant increase in melanocyte ICAM-1 expression was 1 U/ml IFN-G, 0.3 ng/ml TNF-alpha, or 3 U/ml IL-1 alpha. Melanocytes were most sensitive to TNF-alpha stimulation, with the greatest levels of ICAM-1 expression following 30 ng/ml or more TNF-alpha. When IFN-G was added to melanocyte cultures in combination with TNF-alpha or IL-1 alpha, there was an additive increase in ICAM-1 expression but no synergy was noted with the combined cytokines. To our knowledge, this is the first report of melanocyte ICAM-1 induction by TNF-alpha and IL-1 alpha and by physiologically relevant doses of IFN-G. Because of the importance of ICAM-1 in the regulation of immune cell-target interactions, the study of ICAM-1 expression by melanocytes may help us to better understand immune mechanisms of melanocyte injury.
Yohn
J J
JJ
Department of Dermatology, University of Colorado School of Medicine, Denver.
Critelli
M
M
Lyons
M B
MB
Norris
D A
DA
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D001685:Biological Factors / Q000494:pharmacology*; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000096:biosynthesis*; D002478:Cells, Cultured; D016207:Cytokines; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007231:Infant, Newborn; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma / Q000494:pharmacology*; D007375:Interleukin-1 / Q000494:pharmacology*; D007546:Isoquinolines / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D008544:Melanocytes / Q000187:drug effects / Q000276:immunology*; D010879:Piperazines / Q000494:pharmacology; D047428:Protein Kinase Inhibitors; D011991:Receptors, Virus / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D001685:Biological Factors; D015815:Cell Adhesion Molecules; D016207:Cytokines; D007375:Interleukin-1; D007546:Isoquinolines; D010879:Piperazines; D047428:Protein Kinase Inhibitors; D011991:Receptors, Virus; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D013755:Tetradecanoylphorbol Acetate