Man-made antibodies.
Monoclonal antibodies can now be genetically engineered and endowed with new properties. In the future, gene technology could enable antigen-binding fragments to be made by exploiting repertoires of variable domain genes derived from immunized animals and expressed in bacteria. How readily can this approach be extended to production of 'in vitro' repertoires of variable domain genes, and obviate the immunization of animals?
Winter
G
G
MRC Laboratory of Molecular Biology, Cambridge, UK.
Milstein
C
C
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D000916:Antibody Diversity; D000941:Antigens / Q000276:immunology; D001666:Binding Sites, Antibody; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D005818:Genetic Engineering; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007135:Immunoglobulin Variable Region / Q000737:chemistry / Q000276:immunology; D046911:Macromolecular Substances; D051379:Mice; D015394:Molecular Structure; D016296:Mutagenesis; D011487:Protein Conformation; D051381:Rats
D000911:Antibodies, Monoclonal; D000941:Antigens; D007135:Immunoglobulin Variable Region; D046911:Macromolecular Substances