Metabolism of the carcinogen 3-methylcholanthrene in human bone marrow preparations.
The metabolism of 3-methylcholanthrene was investigated by incubating the hydrocarbon with human bone marrow preparations in air for 60 min at 37 degrees C. The major metabolites were identified by HPLC and GC/MS as 1-hydroxy-3-methylcholanthrene, 1-keto-3-methylcholanthrene, and cholanthrene. The results demonstrate that the potent carcinogen, 3-methylcholanthrene, can undergo biochemical reactions in preparations of human bone marrow, giving rise to the formation of metabolites which are known to be carcinogenic in rats and mice.
Myers
S R
SR
Department of Pharmacology, University of Kentucky.
Flesher
J W
JW
D001711:Biotransformation; D001853:Bone Marrow / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003640:Dealkylation; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008748:Methylcholanthrene / Q000378:metabolism*; D010084:Oxidation-Reduction
D008748:Methylcholanthrene