title: Molecular cloning and sequencing of the epidermal cell differentiation inhibitor gene from Staphylococcus aureus.
abstract: We recently purified to homogeneity a protein inhibiting differentiation of cultured keratinocytes from extracellular products of Staphylococcus aureus, and named it epidermal cell differentiation inhibitor (EDIN). In the present study, we isolated and sequenced the structural gene coding for EDIN from Staphylococcus aureus E-1 using oligonucleotide probes on the basis of the partial amino acid sequence of the purified EDIN. DNA sequencing of the cloned DNA revealed an open reading frame encoding 247 amino acids as a precursor of EDIN, which included an NH2-terminal signal sequence of 35 amino acid residues. Processing of this precursor produces a mature EDIN protein composed of 212 amino acids with a calculated Mr of 23,782. The EDIN shared 35% amino acid homology with the ADP-ribosyltransferase C3 of Clostridium botulinum. These results with biological properties of EDIN described previously indicate that EDIN is a novel protein.
authors.affiliation: Department of Microbiology, Hiroshima University School of Dentistry, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Inoue
authors.forename: M
authors.initials: M
authors.lastname: Sugai
authors.forename: Y
authors.initials: Y
authors.lastname: Murooka
authors.forename: S Y
authors.initials: SY
authors.lastname: Paik
authors.forename: Y M
authors.initials: YM
authors.lastname: Hong
authors.forename: H
authors.initials: H
authors.lastname: Ohgai
authors.forename: H
authors.initials: H
authors.lastname: Suginaka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002454:Cell Differentiation; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D015603:Keratinocytes / Q000166:cytology; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012689:Sequence Homology, Nucleic Acid; D013211:Staphylococcus aureus / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D015345:Oligonucleotide Probes; C067120:epidermal cell differentiation inhibitor, Staphylococcus aureus