title: The DNA sequence and structural organization of the GC2 plasmid from the red alga Gracilaria chilensis.
abstract: The complete DNA sequence of the circular GC2 plasmid from the red alga Gracilaria chilensis was obtained. It contains 3827 bp and has a base composition of 75% A + T nucleotides. The sequence revealed that GC2 has two inverted repeats, each of 290 nucleotides, that border four long direct tandem repeats of 216 nucleotides. five short, direct, tandem repeats of 21-22 nucleotides were also found in the plasmid. The plasmid sequence has one major open reading frame that could encode a 411 amino acid polypeptide. Finally, the GC2 plasmid is transcriptionally active.
authors.affiliation: Atlantic Research Laboratory, National Research Council of Canada, Halifax, Nova Scotia.
authors.forename: R
authors.initials: R
authors.lastname: Villemur
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004270:DNA, Circular / Q000235:genetics; D017343:Genes, Plant; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010940:Plant Proteins / Q000235:genetics; D010957:Plasmids; D011061:Poly A / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D000461:Rhodophyta / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D004270:DNA, Circular; D010940:Plant Proteins; D012333:RNA, Messenger; D011061:Poly A