title: The nucleolar localisation signal of the HTLV-I protein p27rex is important for stabilisation of IL-2 receptor alpha subunit mRNA by p27rex.
abstract: In this study we investigated the mechanism of stabilisation of IL-2 receptor alpha subunit mRNA by the HTLV-I protein p27rex. We tested the role of the nucleolar targetting signal in rex by introducing mutations. Three deletion mutants could not express rex protein in the nucleolus and although protein was still expressed in the nucleoplasm none of the mutants could stabilise IL-2R alpha mRNA. A substitution mutant could be expressed in the nucleolus and could also stabilise IL-2R alpha mRNA. The data show that the nucleolar targetting signal is crucial for stabilisation of IL-2R alpha mRNA by rex and raise the possibility that transport of mRNA from nucleus to cytoplasm can involve the nucleolus.
authors.affiliation: Department of Medical Chemistry, Kyoto University Faculty of Medicine, Japan.
authors.forename: K N
authors.initials: KN
authors.lastname: White
authors.forename: T
authors.initials: T
authors.lastname: Nosaka
authors.forename: H
authors.initials: H
authors.lastname: Kanamori
authors.forename: M
authors.initials: M
authors.lastname: Hatanaka
authors.forename: T
authors.initials: T
authors.lastname: Honjo
mesh_terms: D000818:Animals; D002460:Cell Line; D002466:Cell Nucleolus / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D016370:Gene Products, rex / Q000032:analysis / Q000378:metabolism*; D016355:Genes, pX; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D046911:Macromolecular Substances; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics*; D015375:Receptors, Interleukin-2 / Q000235:genetics*; D015398:Signal Transduction; D014162:Transfection
chemical_list: D016370:Gene Products, rex; D046911:Macromolecular Substances; D012333:RNA, Messenger; D015375:Receptors, Interleukin-2