Liver, lung and kidney homogenates used as an activation system in mutagenicity studies of airborne particles and of expectorate and urine samples from exposed workers in a coke plant.
A comparison was made between lung and kidney homogenates on the one hand and liver S9 from rats on the other hand in order to compare their ability to activate promutagens. The Salmonella reversion assay was used on extracts of airborne particles from the top of coke oven batteries, and of expectorate and urine samples from exposed workers in the same coke plant. The contents of benzo[a]anthracene and benzo[a]pyrene in the different test solutions were measured by high-resolution gas chromatography/mass spectrometry. Both mutagens were detected in the filter extract and in the expectorates from the exposed workers but not in the expectorates from the control groups or in the urine samples. The liver S9 gave significantly higher mutagenicity than lung and kidney activation with both filter samples and expectorate and urine samples.
Krøkje
A
A
Department of Botany, University of Trondheim, Dragvoll, Norway.
Schmid
R
R
Zahlsen
K
K
D000328:Adult; D000368:Aged; D000818:Animals; D001551:Benz(a)Anthracenes / Q000009:adverse effects* / Q000032:analysis / Q000378:metabolism; D001564:Benzo(a)pyrene / Q000009:adverse effects* / Q000032:analysis / Q000378:metabolism; D001711:Biotransformation; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007221:Industry; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009152:Mutagenicity Tests; D009153:Mutagens; D016273:Occupational Exposure; D051381:Rats; D011919:Rats, Inbred Strains; D013183:Sputum / Q000737:chemistry
D001551:Benz(a)Anthracenes; D009153:Mutagens; D001564:Benzo(a)pyrene