The Booroola F gene mutation in sheep is not located close to the FSH-beta gene.
A cDNA probe for the beta subunit of bovine FSH (FSH-beta) detects multiple restriction fragment length polymorphisms (RFLPs) in sheep genomic DNA consistent with an insertion/deletion polymorphism around the FSH-beta locus. The presence of the insertion/deletion was confirmed by screening over 100 individuals with two restriction enzymes detecting RFLPs. All individuals showed the same patterns of fragments with both enzymes. A partial restriction map of the FSH-beta gene in sheep suggests that the insertion/deletion is approximately 2 kb in size and located downstream from the third exon. Individual DNA samples were analysed from two flocks where the Booroola F gene is known to be segregating. Individuals that were heterozygous for the F gene were shown to be homozygous for one or other of the two alleles. Genetic recombination between the FSH-beta locus and the F gene was observed in four pedigrees and there was no evidence that the insertion/deletion is closely linked genetically to the Booroola F gene. A major gene transcript of 2.2-2.3 kb was detected on Northern blots of sheep RNA. Neither the insertion/deletion polymorphism nor the presence of the F gene appeared to influence the size of the FSH-beta gene transcript.
Montgomery
G W
GW
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
Sise
J A
JA
Greenwood
P J
PJ
Fleming
J S
JS
D000483:Alleles; D000818:Animals; D002872:Chromosome Deletion; D003433:Crosses, Genetic; D004247:DNA / Q000235:genetics; D005260:Female; D005640:Follicle Stimulating Hormone / Q000235:genetics*; D037201:Follicle Stimulating Hormone, beta Subunit; D005809:Genes, Regulator; D008040:Genetic Linkage; D008297:Male; D009154:Mutation; D010060:Ovulation; D010375:Pedigree; D010903:Pituitary Gland, Anterior / Q000378:metabolism; D012150:Polymorphism, Restriction Fragment Length; D012756:Sheep / Q000145:classification / Q000235:genetics*
D037201:Follicle Stimulating Hormone, beta Subunit; D005640:Follicle Stimulating Hormone; D004247:DNA