Determinants of sequence-specific DNA-binding by p48v-myb.
The v-myb oncogene of the avian myeloblastosis virus encodes a nuclear protein, p48v-myb, which binds to DNA in a sequence-specific manner. We have used wild type and mutant forms of this protein expressed in E. coli to study the protein and DNA determinants for sequence-specific DNA-binding. We have shown that only the highly conserved domain at the amino terminus of p48v-myb is required for sequence-specific DNA-binding. However, neither of the tandem 50 amino acid repeats present in this domain is alone sufficient for such binding. We have also demonstrated that p48v-myb can recognize a single consensus myb binding site and appears to interact with DNA as a protein monomer. In addition, we have shown that sequence-specific binding by p48v-myb requires nucleotides which flank the previously reported PyAACT/GG consensus.
Garcia
A
A
Department of Microbiology, School of Medicine, State University of New York, Stony Brook 11794-8621.
LaMontagne
K
K
Reavis
D
D
Stober-Grässer
U
U
Lipsick
J S
JS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D008969:Molecular Sequence Data; D020626:Oncogene Proteins v-myb; D011817:Rabbits; D015682:Retroviridae Proteins, Oncogenic / Q000378:metabolism*
D020626:Oncogene Proteins v-myb; D015682:Retroviridae Proteins, Oncogenic; D004247:DNA