1,25 (OH)2 vitamin D3 attenuates the loss of resistance artery contractile function associated with incubation in culture media.
Contractile properties of rat mesenteric resistance arteries were studied immediately after isolation or after 48-hr incubation in culture medium [Dulbecco's modified Eagle's/Ham's F-12 (1:1) with insulin, transferrin and antibiotics]. Incubation in culture medium depressed active stress generating capacity, increased sensitivity to norepinephrine and ablated endothelium-dependent relaxation. The decrease in stress generation results from the loss of a releasable pool of intracellular Ca2+; the enhanced sensitivity is associated with decreased neuronal amine pump function. Addition of 300 pg/ml 1,25 (OH)2 vitamin D3 to the culture medium afforded nearly complete protection against the loss of stress generating capacity and partially preserved endothelial function. It is concluded that 1,25 (OH)2 vitamin D3 partially prevents phenotypic modulation of the vascular myocyte induced by culture conditions.
Xue
H
H
Department of Medicine, Oregon Health Sciences University, Portland 97201.
McCarron
D A
DA
Bukoski
R D
RD
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002117:Calcitriol / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D003470:Culture Media; D004730:Endothelium, Vascular / Q000187:drug effects; D008297:Male; D008638:Mesenteric Arteries / Q000187:drug effects; D009119:Muscle Contraction / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D009638:Norepinephrine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D014655:Vascular Resistance / Q000187:drug effects
D003470:Culture Media; D002117:Calcitriol; D000109:Acetylcholine; D002118:Calcium; D009638:Norepinephrine