[Role of central endogenous dynorphin in control of ACTH and prolactin release during resting and stress].
To determine the role of central endogenous dynorphin (Dyn) in resting and stress-induced release of ACTH and prolactin (PRL), Dyn (1-17) antiserum (AS) or normal rabbit serum (NRS) were microinjected into the 3rd ventricle of freely-moving male rats. Plasma ACTH and PRL levels were measured by radioimmunoassay (RIA) using RIA kits supplied by NIADDK. Our results clearly indicate that Dyn (1-17) AS and NRS had no effect on resting secretion of ACTH and PRL, and restraint stress-induced release of ACTH and PRL were simultaneously lowered significantly in the Dyn (1-17) AS-treated group as compared with the NRS-treated group. The results suggest that central endogenous dynorphin plays an important role in stimulating the concomitant release of ACTH and PRL under certain stresses.
Xu
R K
RK
Institute of Basic Medical Sciences, Beijing.
D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D000818:Animals; D004399:Dynorphins / Q000276:immunology / Q000502:physiology*; D007106:Immune Sera / Q000494:pharmacology; D007276:Injections, Intraventricular; D008297:Male; D011388:Prolactin / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012146:Rest; D012149:Restraint, Physical; D013312:Stress, Physiological / Q000503:physiopathology*
D007106:Immune Sera; D004399:Dynorphins; D000324:Adrenocorticotropic Hormone; D011388:Prolactin