Analysis of isoactin expression in cultured mouse cells by in situ hybridization.
The expression of cytoskeletal isoactin genes in NIH 3T3 and L cells was studied by in situ hybridization. Anti-sense RNA probes specific for beta- and gamma-actin were prepared by in vitro transcription of the 3'-untranslated regions of each cDNA which diverge in an isoactin-specific manner. Both NIH 3T3 and L cells showed a higher expression of beta- and gamma-actin mRNAs in a growing state than in a stationary one. Among these changes, the suppression of gamma-actin mRNA in L cells was most prominent and that of beta-actin in 3T3 cells was least profound.
Nakamura
Y
Y
Division of Biochemistry, Chiba Cancer Center Research Institute, Japan.
Sakiyama
S
S
D000199:Actins / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism*; D005786:Gene Expression Regulation; D007739:L Cells / Q000378:metabolism*; D051379:Mice; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000096:biosynthesis; D015347:RNA Probes; D016372:RNA, Antisense; D012333:RNA, Messenger / Q000096:biosynthesis
D000199:Actins; D015347:RNA Probes; D016372:RNA, Antisense; D012333:RNA, Messenger; D011061:Poly A; D004247:DNA