title: Differential expression of a family of putative adenylate/guanylate cyclase genes in Trypanosoma brucei.
abstract: The expression site for the variant surface glycoprotein (VSG) gene of Trypanosoma brucei contains several genes of unknown function (ESAGs, for expression site-associated genes). Among these, ESAG 4 shows homology to eukaryotic adenylate/guanylate cyclase genes, in the region encoding the presumptive enzyme catalytic domain. This gene belongs to a family of related sequences, and hybridizes to the genomic DNA of other trypanosomatids, such as Trypanosoma congolense, Trypanosoma vivax and Trypanosoma mega. While ESAG 4 is transcribed only in bloodstream forms by a RNA polymerase resistant to alpha-amanitin, at least three other members of this family are transcribed in both bloodstream and procyclic forms, by a RNA polymerase sensitive to the drug. These genes encode different putative transmembrane proteins showing high sequence conservation in the region corresponding to the adenylate/guanylate cyclase catalytic domain.
authors.affiliation: Department of Molecular Biology, University of Brussels, Rhode St.-Genèse, Belgium.
authors.forename: S
authors.initials: S
authors.lastname: Alexandre
authors.forename: P
authors.initials: P
authors.lastname: Paindavoine
authors.forename: P
authors.initials: P
authors.lastname: Tebabi
authors.forename: A
authors.initials: A
authors.lastname: Pays
authors.forename: S
authors.initials: S
authors.lastname: Halleux
authors.forename: M
authors.initials: M
authors.lastname: Steinert
authors.forename: E
authors.initials: E
authors.lastname: Pays
mesh_terms: D000262:Adenylyl Cyclases / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015971:Gene Expression Regulation, Enzymologic; D006162:Guanylate Cyclase / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014346:Trypanosoma brucei brucei / Q000201:enzymology / Q000235:genetics*; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000235:genetics
chemical_list: D014643:Variant Surface Glycoproteins, Trypanosoma; D000262:Adenylyl Cyclases; D006162:Guanylate Cyclase