Chemical characterization of Campylobacter jejuni lipopolysaccharides containing N-acetylneuraminic acid and 2,3-diamino-2,3-dideoxy-D-glucose.
Lipopolysaccharides (LPS) of four nonencapsulated strains of the human enteric pathogen Campylobacter jejuni were chemically characterized. When applied to two of the strains, extraction by a modified phenol-chloroform-petroleum ether method (H. Brade and C. Galanos, Eur. J. Biochem. 122:233-237, 1982) gave better yields of LPS than did extraction by the conventional hot phenol-water technique. Constituents common to all LPS were D-glucose, D-galactose, L-glycero-D-manno-heptose, 3-deoxy-D-manno-2-octulosonic acid, D-glucuronic acid, D-galactosamine, and phosphorylethanolamine. Phosphate was present in a relatively high amount. In addition, the LPS of three strains contained N-acetylneuraminic acid, whereas the LPS of the strain lacking this component contained 3-amino-3,6-dideoxy-D-glucose. The lipid A component contained phosphate with D-glucosamine and 2,3-diamino-2,3-dideoxy-D-glucose as the major amino sugars. Ethanolamine-phosphate was present also. The major fatty acids were ester- and amide-bound 3-hydroxytetradecanoic and ester-bound hexadecanoic acids, with a minor amount of ester-bound tetradecanoic acid. This is the first report of N-acetylneuraminic acid in the oligosaccharide moiety and diaminoglucose in the lipid A of C. jejuni LPS.
Moran
A P
AP
Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, Federal Republic of Germany.
Rietschel
E T
ET
Kosunen
T U
TU
Zähringer
U
U
D016123:Campylobacter jejuni / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis; D004590:Electrophoresis, Paper; D019856:Ethanolamine; D004983:Ethanolamines / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D005944:Glucosamine / Q000031:analogs & derivatives* / Q000032:analysis; D008070:Lipopolysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase; D010710:Phosphates / Q000032:analysis; D012794:Sialic Acids / Q000032:analysis*; D013403:Sugar Phosphates / Q000032:analysis
D002241:Carbohydrates; D004983:Ethanolamines; D005227:Fatty Acids; D008070:Lipopolysaccharides; D010710:Phosphates; D012794:Sialic Acids; D013403:Sugar Phosphates; C040138:2,3-diamino-2,3-dideoxyglucose; D019856:Ethanolamine; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid; D005944:Glucosamine