Mechanisms for muscarinic inhibition of somatostatin release from canine fundic D cells.
We undertook the present studies to explore the mechanisms by which carbachol inhibits the release of somatostatin-like immunoreactivity (SLI) from D cells. D cells were isolated from canine fundic mucosa by collagenase/EDTA dispersion followed by counterflow elutriation. Carbachol inhibited the release of SLI induced by forskolin, dibutyryl 3':5' cyclic adenosine monophosphate (cAMP), pentagastrin (PG), and 12-0-tetradecanoyl-phorbol-13-acetate in a fashion that could be prevented by pertussis toxin (PT) pretreatment of the D cells. Pertussis toxin also prevented the carbachol-induced inhibition of forskolin-stimulated cAMP generation and PG-stimulated [Ca2+]i mobilization. These data indicate that pertussis toxin sensitive inhibitory guanine nucleotide binding proteins mediate many of carbachol's inhibitory actions on D cells.
Chiba
T
T
Department of Internal Medicine, Kobe University School of Medicine, Japan.
Yamada
T
T
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D004285:Dogs; D005748:Gastric Fundus / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D010277:Parasympathomimetics / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism; D013004:Somatostatin / Q000378:metabolism*
D010277:Parasympathomimetics; D013004:Somatostatin; D000242:Cyclic AMP; D011493:Protein Kinase C; D002118:Calcium