AGM1+ spleen cells contain gamma interferon (IFN-gamma) gene transcripts in the early, sex-dependent production of IFN-gamma after picornavirus infection.
Encephalomyocarditis D variant (EMCV-D)-infected spleen cell cultures prepared from diabetes-resistant ICR Swiss female mice produce more gamma interferon (IFN-gamma) activity over a 24-h period than do spleen cell cultures from diabetes-susceptible male mice of this strain. Pretreatment of mice with anti-asialo GM1 eliminates early in vitro IFN-gamma production from 4 to 16 h postinfection (p.i.) and reduces IFN-gamma production from 16 to 24 h p.i. In this study, depletion of spleen cells with anti-Thy-1 by panning greatly reduced IFN-gamma activity in EMCV-D-infected spleen cell cultures throughout a 24-h period. Populations of asialo GM1 (AGM1), L3T4, and Lyt-2-positive cells were isolated from cells harvested at 9 h p.i. from EMCV-D-infected spleen cell cultures by a modified panning technique on polystyrene microscope slides. By in situ hybridization with a [35S]dATP-labeled IFN-gamma cDNA probe, only the AGM1-bearing cells were found to contain detectable IFN-gamma gene transcripts. An AGM1+, Thy-1+ natural killer-like cell is the probable producer of the early, sex-dependent IFN-gamma activity in this system.
McFarland
H I
HI
Department of Microbiology and Immunology, School of Medicine, Wright State University, Dayton, Ohio 45435.
Bigley
N J
NJ
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D002478:Cells, Cultured; D015342:DNA Probes; D004686:Encephalomyocarditis virus / Q000235:genetics / Q000276:immunology*; D005260:Female; D005677:G(M1) Ganglioside; D006028:Glycosphingolipids / Q000032:analysis*; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics*; D007700:Kinetics; D008212:Lymphocyte Depletion; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009693:Nucleic Acid Hybridization; D013154:Spleen / Q000276:immunology*; D018800:Thy-1 Antigens; D014158:Transcription, Genetic
D000954:Antigens, Surface; D015342:DNA Probes; D006028:Glycosphingolipids; D018800:Thy-1 Antigens; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside; D007371:Interferon-gamma