Use of LeuM1 monoclonal antibody for the diagnosis of Hodgkin's disease.
Monoclonal antibody to LeuM1, a granulocyte-related differentiation antigen, represents a highly effective reagent for detection of diagnostic "Reed-Sternberg" cells and variants in paraffin-embedded tissues of Hodgkin's disease. The "Reed Sternberg" cell in all the cases of Hodgkin's disease except lymphocyte predominance variety revealed positive intracytoplasmic/paranuclear granular staining with LeuM1 marker. The R-S cells in lymphocyte predominance variety contain probably sialylated LeuM1 antigen. All the cases of non-Hodgkin's lymphoma and reactive lymphadenitis showed no staining with LeuM1 monoclonal antibody. Therefore this antibody represents a potentially helpful diagnostic discriminant in the assessment of Hodgkin's disease and its distinction from non-Hodgkin's lymphomas and morphologically similar reactive lymphoid lesions.
Memon
G M
GM
Department of Pathology, Jinnah Postgraduate Medical Centre, Karachi.
Alam
S M
SM
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm / Q000032:analysis*; D015703:Antigens, CD / Q000276:immunology*; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology*; D014408:Biomarkers, Tumor; D002648:Child; D003937:Diagnosis, Differential; D006689:Hodgkin Disease / Q000175:diagnosis*; D006801:Humans; D016256:Lewis X Antigen; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis; D008297:Male
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D014408:Biomarkers, Tumor; D016256:Lewis X Antigen