Direct effects of serotonin, and 5-HT2, alpha 1 and H1 receptor-antagonists on embryonic chick skin in vitro: a morphological and functional study.
Ketanserin (K), a 5-HT2, alpha 1 and H1 receptor antagonist, at a concentration of 5 micrograms/ml inhibits keratinisation, but increases DNA-, collagen and glycosaminoglycan (GAG) synthesis in embryonic chick skin in vitro. On the other hand, serotonin (S) at a concentration of 10 micrograms/ml stimulates keratinisation and does not enhance DNA, collagen and GAG synthesis. 5 nM doses of ketanserin and serotonin give analogous results. The effects of other 5-HT2-receptor antagonists (e.g. BW 501 and cinanserin) are comparable to those of K, whereas prazosin (an alpha 1 receptor antagonist) and pyrilamin (an H1 receptor antagonist) do not show any effect on embryonic chick skin in vitro.
Beele
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Laboratory for Histology, State University of Ghent, Belgium.
Thierens
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de Ridder
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D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology*; D000818:Animals; D002642:Chick Embryo; D003094:Collagen / Q000096:biosynthesis; D005944:Glucosamine / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis; D006634:Histamine H1 Antagonists / Q000494:pharmacology*; D066298:In Vitro Techniques; D007650:Ketanserin / Q000494:pharmacology*; D011392:Proline / Q000378:metabolism; D012701:Serotonin / Q000494:pharmacology*; D012702:Serotonin Antagonists; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D000317:Adrenergic alpha-Antagonists; D006025:Glycosaminoglycans; D006634:Histamine H1 Antagonists; D012702:Serotonin Antagonists; D014316:Tritium; D012701:Serotonin; D003094:Collagen; D007650:Ketanserin; D011392:Proline; D005944:Glucosamine; D013936:Thymidine