pap-2-encoded fimbriae adhere to the P blood group-related glycosphingolipid stage-specific embryonic antigen 4 in the human kidney.
A subtype of P fimbriae, encoded by the pap-2 gene cluster, has been analyzed for agglutination of erythrocytes and for binding to cryostat sections of the human kidney. We have demonstrated that pap-2-encoded fimbriae are capable of binding to erythrocytes from some animal species and to human erythrocytes which express globoside and the LKE (stage-specific embryonic antigen 4 [SSEA-4]) antigen. The pap-2 fimbriae bind to Bowman's capsule in the human kidney. Monoclonal antibodies directed against glycosphingolipids were used for the detection of specific P blood group-related antigens in the human kidney and on erythrocytes. Preincubation of kidney sections with monoclonal antibody MC813-70, which binds to the SSEA-4 antigen, inhibited adherence of purified pap-2-encoded fimbriae to Bowman's capsule. We suggest that one receptor for pap-2-encoded fimbriae is the antigen known as LKE (Luke) on human erythrocytes or SSEA-4 in the tissues.
Karr
J F
JF
Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77030.
Nowicki
B J
BJ
Truong
L D
LD
Hull
R A
RA
Moulds
J J
JJ
Hull
S I
SI
D000911:Antibodies, Monoclonal / Q000276:immunology; D000941:Antigens / Q000032:analysis*; D001422:Bacterial Adhesion; D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology; D010861:Fimbriae, Bacterial / Q000276:immunology*; D006028:Glycosphingolipids / Q000276:immunology*; D006384:Hemagglutination; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney / Q000382:microbiology*; D008969:Molecular Sequence Data; D010127:P Blood-Group System; D055746:Stage-Specific Embryonic Antigens
D000911:Antibodies, Monoclonal; D000941:Antigens; D006028:Glycosphingolipids; D010127:P Blood-Group System; D055746:Stage-Specific Embryonic Antigens; C066584:stage-specific embryonic antigen-4