Translocation of the yeast dolichol-phosphate-mannose synthase into microsomal membranes.
Dolichol-phosphate-mannose synthase catalyzes the formation of Dolichol-phosphate-mannose from Dolichol-phosphate and GDP-mannose. Analysis of the primary amino acid sequence of the yeast enzyme predicts a luminal orientation of the enzyme in the endoplasmic reticulum. We analysed the translocation of the Dolichol-phosphate-mannose synthase into dog pancreatic microsomal membranes: resistance to proteolytic attack provides evidence of its luminal orientation and asks for a reevaluation of the topology of the reaction.
Déglon
N
N
Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.
Krapp
A
A
Bron
C
C
Fasel
N
N
D000818:Animals; D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D004285:Dogs; D005800:Genes, Fungal; D007425:Intracellular Membranes / Q000201:enzymology; D008364:Mannosyltransferases / Q000235:genetics* / Q000302:isolation & purification; D008861:Microsomes / Q000201:enzymology*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010179:Pancreas / Q000201:enzymology; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D015183:Restriction Mapping; D012156:Reticulocytes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D014158:Transcription, Genetic
D004271:DNA, Fungal; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D008364:Mannosyltransferases; C022755:dolichyl-phosphate beta-D-mannosyltransferase