Evaluation of API Coryne in comparison with conventional methods for identifying coryneform bacteria.
A study was performed to evaluate a new manual miniaturized system, API Coryne (API-bioMérieux, Inc., La Balme les Grottes, France), in which conventional biochemical methods were used to identify 240 isolates of coryneform and related bacteria. A total of 40% of the isolates were excluded from the study because they could not be identified by conventional methods. Identifications of the 240 isolates obtained with API Coryne showed a 97.6% concordance with conventional methods (79% after 24 h of incubation, 21% after 48 h of incubation): 158 (65.8%) isolates were identified with no further testing, and extra testing was required for 76 (31.8%) isolates. In three (1.2%) cases, the organisms did not correspond to any key in the code book and could not be identified by the computer service of the manufacturer. Only three (1.2%) strains were misidentified. The system was shown to be reliable and rapid when compared with standard identification methods.
Freney
J
J
Laboratoire de Bactériologie, Faculté de Pharmacie, Lyon, France.
Duperron
M T
MT
Courtier
C
C
Hansen
W
W
Allard
F
F
Boeufgras
J M
JM
Monget
D
D
Fleurette
J
J
D001431:Bacteriological Techniques; D003352:Corynebacterium / Q000145:classification*; D005069:Evaluation Studies as Topic; D006801:Humans