Receptors for the Fc portion of immunoglobulins (FcR) on murine oral mucosal T cells.
The incidence and distribution of immunoglobulin receptors on oral mucosal T cells were investigated. Enrichment of either Thy-1.2, L3T4 or Lyt-2.2-positive cells was achieved by the use of a panning procedure following cell extraction by an optimized digestion method that preserved all three T-cell markers. The percentage of cells possessing Fc receptors was determined by using immunoglobulin-coated (IgM, IgG or IgA) fluorescent microspheres in a multipoint rosetting assay. We report that approximately 60% of Thy-1.2, L3T4 and Lyt-2.2 positive cells bear Fc gamma R whereas less than 5% of T cells of any subset bear Fc alpha R or Fc mu R. In frozen tissue sections, Fc gamma R+ cells were mainly scattered throughout the basal and suprabasal epithelium and were observed at a lower frequency in the minor salivary gland network. From their distribution, it is anticipated that Fc gamma R+ cells may be involved in the surveillance of the epithelium while the minor Fc alpha R+ L3T4+ T lymphocyte population may promote the expression of sIgA by resident sIgM-bearing B cells, and their differentiation into IgA plasma cells.
Bolduc
C
C
Groupe de Recherche en Ecologie Buccale (GREB) Dental School, Université Laval, Québec, Canada.
Deslauriers
N
N
D000818:Animals; D000950:Antigens, Ly / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D002469:Cell Separation; D005455:Fluorescent Antibody Technique; D007141:Immunoglobulin Fc Fragments / Q000032:analysis*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D009061:Mouth Mucosa / Q000737:chemistry*; D011961:Receptors, Fc / Q000032:analysis*; D012397:Rosette Formation; D013154:Spleen / Q000737:chemistry / Q000166:cytology; D016176:T-Lymphocyte Subsets / Q000737:chemistry*; D018800:Thy-1 Antigens
D000950:Antigens, Ly; D000954:Antigens, Surface; D007141:Immunoglobulin Fc Fragments; D011961:Receptors, Fc; D018800:Thy-1 Antigens