title: Properties of B cells and Thy-1-antigen-expressing cells infiltrating rat renal allografts.
abstract: We have examined (1) the frequency of B cells secreting antibodies against donor major histocompatibility complex (MHC) antigens and (2) the properties of Thy-1-antigen-expressing leukocytes in rats rejecting renal allografts. Our results show that B cells secreting antibodies are present in the inflammatory cell population at the frequency of 1:850. Among them only 1 out of 2-150 is engaged in production of antibodies directed to the graft MHC antigens, depending on the method of assay. This suggests that despite the observed significant production of nonspecific immunoglobulin in situ, only a minority of the B-cell population is specifically committed to the graft MHC antigens. This finding is concordant with the described previously low frequencies of the T cells specifically directed toward the graft MHC antigen. The role of the "immunologically noncommitted" cells in graft rejection is unknown. We have found that a substantial part (up to 60%) of inflammatory cells invading a rat kidney allograft express the Thy-1 antigen. This suggests that they might be immature (progenitor?) cells and, therefore, unable to respond to the graft antigens. Progenitor-like properties of these cells have been confirmed by their ability to reconstitute lethally irradiated syngeneic rat. Finally, these immature cells are of lymphoid, not of myeloid, linkage, because they do not proliferate in the presence of GM-CSF.
authors.affiliation: Transplantation Laboratory, University of Helsinki, Finland.
authors.forename: D
authors.initials: D
authors.lastname: Leszczynski
authors.forename: J
authors.initials: J
authors.lastname: Halttunen
authors.forename: S
authors.initials: S
authors.lastname: Tiisala
authors.forename: J
authors.initials: J
authors.lastname: Ustinov
authors.forename: R
authors.initials: R
authors.lastname: Renkonen
authors.forename: P
authors.initials: P
authors.lastname: Häyry
mesh_terms: D000818:Animals; D000921:Antibody-Producing Cells / Q000473:pathology; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000473:pathology*; D006084:Graft Rejection; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000473:pathology; D007249:Inflammation; D016030:Kidney Transplantation / Q000276:immunology / Q000473:pathology*; D011828:Radiation Chimera; D051381:Rats; D011920:Rats, Inbred WF / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000473:pathology; D013601:T-Lymphocytes / Q000473:pathology*; D018800:Thy-1 Antigens; D014184:Transplantation, Homologous / Q000473:pathology
chemical_list: D000954:Antigens, Surface; D018800:Thy-1 Antigens