Localization and polymorphism of a chromosome 12-specific alpha satellite DNA sequence.
The isolation and localization of a chromosome 12-specific alpha satellite DNA sequence, p alpha 12H8, is described. This clone contains a complete copy of the 1.4-kb HindIII higher-order repeat present within the alpha satellite array on chromosome 12. The specificity of p alpha 12H8 was demonstrated by in situ hybridization and Southern blot analysis of a somatic cell hybrid mapping panel, both performed under high-stringency conditions. Polymorphic restriction patterns within the alpha satellite array, revealed by the use of the restriction enzymes BglII and EcoRV, were demonstrated to display Mendelian inheritance. These properties make p alpha 12H8 a valuable genetic marker for the centromeric region of chromosome 12.
Looijenga
L H
LH
Department of Pathology, University of Groningen, The Netherlands.
Smit
V T
VT
Wessels
J W
JW
Mollevanger
P
P
Oosterhuis
J W
JW
Cornelisse
C J
CJ
Devilee
P
P
D002503:Centromere; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D015342:DNA Probes; D004276:DNA, Satellite / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length
D015342:DNA Probes; D004276:DNA, Satellite