title: Cultured epithelial cells from patients with cystic fibrosis have an increased expression of the 14 kDa Ca2(+)-binding protein CFA.
abstract: The Cystic Fibrosis antigen (CFA) is a 14 kDa. Ca2(+)-binding protein known to be expressed in cells of myeloid origin during normal cell differentiation. CFA serum levels are elevated in Cystic Fibrosis (CF) patients and heterozygotes. We examined the expression of CFA in different cultured epithelial cells from controls and patients with CF. The steady state level of CFA was in general higher in epithelial cells from CF patients compared to control cells and was found to increase during cell aging. The latter difference could be attributed to an increased rate of CFA synthesis rather than to an impairment of CFA degradation or secretion, as shown by pulse chase experiments.
authors.affiliation: MGC Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Keulemans
authors.forename: V
authors.initials: V
authors.lastname: Van Heyningen
authors.forename: B J
authors.initials: BJ
authors.lastname: Scholte
authors.forename: J
authors.initials: J
authors.lastname: Bijman
authors.forename: A W
authors.initials: AW
authors.lastname: van der Kamp
authors.forename: M
authors.initials: M
authors.lastname: Kansen
authors.forename: H
authors.initials: H
authors.lastname: De Jonge
authors.forename: H
authors.initials: H
authors.lastname: Galjaard
authors.forename: A T
authors.initials: AT
authors.lastname: Hoogeveen
mesh_terms: D001798:Blood Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D040501:Calgranulin A; D002478:Cells, Cultured; D003550:Cystic Fibrosis / Q000276:immunology / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D006098:Granulocytes / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D007700:Kinetics; D008970:Molecular Weight; D009298:Nasal Polyps / Q000378:metabolism; D012016:Reference Values
chemical_list: D001798:Blood Proteins; D040501:Calgranulin A; D003598:Cytoskeletal Proteins; C026422:cystic fibrosis serum factor; D004815:Epidermal Growth Factor