title: Hexane preserves biological activity of isozymes and DNA.
abstract: Live or frozen insects are required for using isozyme and DNA RFLP methods in studies on population structure, systematics and incrimination of sibling species. Difficulty in keeping insects alive or unavailability of liquid nitrogen or dry ice at regular intervals during extended collection trips poses a serious problem. We describe a method for preserving insects in hexane, under field conditions, for isozyme and DNA analysis.
authors.affiliation: Insects Affecting Man and Animals Research Laboratory, USDA, Agricultural Research Service, Gainesville, FL 32604.
authors.forename: S K
authors.initials: SK
authors.lastname: Narang
authors.forename: J A
authors.initials: JA
authors.lastname: Seawright
mesh_terms: D000818:Animals; D000852:Anopheles / Q000201:enzymology* / Q000235:genetics*; D004247:DNA; D004772:Entomology / Q000379:methods*; D006586:Hexanes; D007527:Isoenzymes; D012150:Polymorphism, Restriction Fragment Length; D014021:Tissue Preservation / Q000379:methods*
chemical_list: D006586:Hexanes; D007527:Isoenzymes; D004247:DNA