Direct oxidation of glutamate by mitochondria from porcine adrenal cortex.
1. Mitochondria isolated from porcine adrenal cortex under State 3 conditions oxidized succinate with a rate of 47 +/- 4.48 na oxygen/min/mg/protein and with ADP:O ratio 0.98 +/- 0.09. In the presence of 15 microM deoxycorticosterone the rate of succinate oxidation was 36.8 +/- 3.08 na oxygen/min/mg/protein. 2. Under the same conditions the rate of glutamate oxidation was 22.8 +/- 2.21 and 16.8 +/- 0.65 na oxygen/min/mg/protein, respectively. ADP:O ratio was 1.45 +/- 0.14. 3. Introduction of trace amounts of malate into the mitochondria oxidizing glutamate only slightly increased the rate of O2 uptake. 4. The glutamate dehydrogenase activity in these mitochondria was 12.5 +/- 0.69 nmol/min/mg.
Popinigis
J
J
Department of Bioenergetics, Academy of Physical Education, Gdansk-Oliwa, Poland.
Antosiewicz
J
J
Mazzanti
L
L
Bertoli
E
E
Lenaz
G
G
Cambria
A
A
D000302:Adrenal Cortex / Q000378:metabolism* / Q000648:ultrastructure; D000818:Animals; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D013057:Spectrum Analysis; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid; D013552:Swine
D005971:Glutamates; D013386:Succinates; D018698:Glutamic Acid; D019802:Succinic Acid; D005969:Glutamate Dehydrogenase