title: Adhesive fimbriae associated with porcine enterotoxigenic Escherichia coli of the O141 serotype.
abstract: Enterotoxigenic Escherichia coli of the O141 serotype, isolated from piglets with postweaning coliform enteritis but producing none of the characterized adhesive fimbriae, was examined for fimbrial production by transmission electron microscopy. Two strains that produced numerous fimbriae were chosen for further characterization. The fimbriae were isolated and purified and had a subunit molecular weight of 17,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Using antiserum raised against this protein, we have shown it to be specific for the 17,000-molecular-weight band by immunoblotting and to be directed against the fimbriae by immunoelectron microscopy. These fimbriae were not produced when the bacteria were grown at 18 degrees C and did not show any mannose-resistant hemagglutination of the erythrocytes tested. We propose that these are a new type of adhesive fimbriae associated with porcine enterotoxigenic E. coli of the O141 serotype.
authors.affiliation: Regional Veterinary Laboratory, Department of Agriculture and Rural Affairs, Bendigo, Victoria, Australia.
authors.forename: R M
authors.initials: RM
authors.lastname: Kennan
authors.forename: R P
authors.initials: RP
authors.lastname: Monckton
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D004768:Enterotoxins / Q000096:biosynthesis; D004926:Escherichia coli / Q000145:classification / Q000472:pathogenicity / Q000648:ultrastructure*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D010861:Fimbriae, Bacterial / Q000648:ultrastructure*; D008854:Microscopy, Electron; D012703:Serotyping; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
chemical_list: D004768:Enterotoxins