Production of alpha-interferon by human leukocytes.
We examined the capacity of human leukocytes to produce alpha-interferon subtypes following viral induction. It was shown that the beta-propiolactone inactivated viral inductor stimulated the production of a complete set of native alpha-interferon subtypes. Acid treatment of native interferon at pH 2.0 inactivated the acid-labile portion of alpha-interferon. Its exposure to 37 degrees C for 5 days and repeated acid treatment at pH 2.0 resulted in additional inactivation of some interferon pool fractions. The acid-labile subtypes of human alpha-interferon were formed in a nonadherent mononuclear cell fraction.
Scheglovitova
O N
ON
Gamaleya Research Institute of Epidemiology and Microbiology of the AMS U.S.S.R., Moscow.
Kulieva
A M
AM
Orlova
T G
TG
D006801:Humans; D006863:Hydrogen-Ion Concentration; D007370:Interferon Type I / Q000096:biosynthesis* / Q000097:blood; D007962:Leukocytes / Q000378:metabolism* / Q000382:microbiology; D009975:Orthomyxoviridae / Q000254:growth & development; D011420:Propiolactone / Q000494:pharmacology; D014775:Virus Activation
D007370:Interferon Type I; D011420:Propiolactone