title: Adaptation to non-ammoniagenic medium and selective substrate feeding lead to enhanced yields in animal cell cultures.
abstract: Methods for the adaptation of three animal cell lines to media in which glutamine is replaced with either glutamate or 2-oxoglutarate are described. The cell lines differ in their rate of adaptation. The consequences of the adaptation of the McCoy cell line to a glutamate-based medium was measured in terms of: cell yield (increased by 17%), ammonia accumulation (reduced by 70%), glucose consumption (decreased by greater than 70%) and lactate accumulation (decreased by greater than 75%). The value of such adaptation and concomitant changes in energy metabolism lies in the potential for increased cell yields. Batch feeding of adapted cells in a microcarrier culture with a cocktail of glucose and amino acids resulted in increases in cell yields of 80% compared to unfed controls.
authors.affiliation: Department of Biological Sciences, Manchester Polytechnic, UK.
authors.forename: T
authors.initials: T
authors.lastname: Hassell
authors.forename: M
authors.initials: M
authors.lastname: Butler
mesh_terms: D000222:Adaptation, Physiological; D000596:Amino Acids; D000641:Ammonia / Q000378:metabolism; D000818:Animals; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism; D005971:Glutamates; D018698:Glutamic Acid; D005973:Glutamine; D007656:Ketoglutaric Acids; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid
chemical_list: D000596:Amino Acids; D003470:Culture Media; D005971:Glutamates; D007656:Ketoglutaric Acids; D007773:Lactates; D005973:Glutamine; D019344:Lactic Acid; D018698:Glutamic Acid; D000641:Ammonia; D005947:Glucose