Chromogenic method for rapid isolation of recA-like mutants of gram-negative bacteria.
We have devised a rapid and widely applicable color test for detecting recA-like mutants of gram-negative bacteria. The technique depends on decreased expression of an Escherichia coli recA-lacZ fusion in recA mutants and uses a broad-host-range plasmid to transfer the fusion gene into new species. We describe the isolation of a recA-like mutant of Pseudomonas syringae by this technique.
Barbe
J
J
Department of Genetics and Microbiology, Autonomous University of Barcelona, Spain.
Fernandez de Henestrosa
A R
AR
Calero
S
S
Gibert
I
I
D004926:Escherichia coli / Q000235:genetics*; D006090:Gram-Negative Bacteria / Q000235:genetics* / Q000302:isolation & purification; D007700:Kinetics; D009154:Mutation; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics* / Q000528:radiation effects; D011938:Rec A Recombinases / Q000096:biosynthesis / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D014466:Ultraviolet Rays
D011993:Recombinant Fusion Proteins; D011938:Rec A Recombinases