Suicide-inhibitory bifunctionally linked substrates (SIBLINKS) as phospholipase A2 inhibitors. Mechanistic implications.
Novel phospholipids that function as mechanism-based inhibitors for phospholipase A2 (PLA2) are described. PLA2-catalyzed hydrolysis of the sn-2 ester of these suicide-inhibitory bifunctionally linked substrates (SIBLINKS) followed by a cyclization reaction generates a cyclic anhydride at the active site of the enzyme which leads to inhibition. Structure/activity relationships for the SIBLINKS substituents in the sn-1 and sn-2 position are delineated. Time courses and efficiency of SIBLINKS inhibition are reported and compared for extracellular PLA2s obtained from Naja naja naja, porcine pancreas, bee venom, Crotalus atrox and Crotalus adamanteus. SIBLINKS-inhibited PLA2s cannot process either monomeric or micellar substrates consistent with inhibition at the catalytic site. Some SIBLINKS efficiently inactivate 1 mol of N. naja naja and C. adamanteus PLA2/6-10 mol of SIBLINKS hydrolyzed. Inhibition of N. naja naja PLA2 can be reversed by hydroxylamine, suggesting that a tyrosine residue is acylated.
Washburn
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Department of Chemistry, University of California, San Diego, La Jolla 92093.
Dennis
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EA
D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D003435:Crotalid Venoms; D006868:Hydrolysis; D019811:Hydroxylamine; D006898:Hydroxylamines; D008246:Lysophospholipids / Q000494:pharmacology*; D010712:Phosphatidic Acids / Q000494:pharmacology*; D010741:Phospholipases A / Q000037:antagonists & inhibitors*; D054467:Phospholipases A2; D010743:Phospholipids / Q000737:chemistry* / Q000302:isolation & purification
D003435:Crotalid Venoms; D006898:Hydroxylamines; D008246:Lysophospholipids; D010712:Phosphatidic Acids; D010743:Phospholipids; D019811:Hydroxylamine; D010741:Phospholipases A; D054467:Phospholipases A2