Cloning and nucleotide sequence of the chimpanzee c-myc gene.
A DNA fragment covering the chimpanzee c-myc locus was cloned from the DNA of peripheral blood lymphocytes, sequenced, and compared to its human c-myc counterpart. The two nucleotide sequences were found to be highly homologous (99%). The divergence rate between the two species was 0.4% in exons and 1.7% in introns. The different TATA-boxes described in the human myc gene were also identified in the chimpanzee sequence and an open reading frame (ORF) was observed which overlaps the chimpanzee c-myc first exon. This latter ORF contained three silent mutations with regard to the human region, whereas the chimpanzee Myc oncoprotein coded by exons 2 and 3 differed by two amino acids from the human one.
Argaut
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C
Laboratoire de Recombinaisons Génétiques, UPR 41 CNRS, Centre Hayem, Hôpital Saint-Louis, Paris, France.
Rigolet
M
M
Eladari
M E
ME
Galibert
F
F
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005091:Exons; D016259:Genes, myc; D014644:Genetic Variation; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D002679:Pan troglodytes; D012689:Sequence Homology, Nucleic Acid