title: Expression of a novel nuclear protein in activated and in tat-I expressing T cells.
abstract: The intracellular events that occur in T lymphoid cells after activation or after infection with HIV-1 are not well defined. In the case of HIV-1 infection, it is unknown whether the tat-I gene, an essential gene for viral replication, affects host cell nuclear factors. Using two-dimensional PAGE, we have identified a novel nuclear protein, designated nuclear protein-28,000 (NP-28), which is induced in Jurkat T cells by stimulation with PMA and/or PHA or ionomycin. This nuclear protein has an apparent molecular mass of 28,000 Da and an isoelectric point of 4.6. Interestingly, Jurkat cells transfected with tat-I express higher levels of NP-28 constitutively, without added stimulation. Incubation of Jurkat cells expressing tat-I with PMA and/or PHA or ionomycin causes superinduction of NP-28. We have therefore identified a novel lymphoid nuclear protein induced by T cell activation that occurs in tat-I expressing cells in the absence of activating agents.
authors.affiliation: Howard Hughes Medical Institute, Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109.
authors.forename: A
authors.initials: A
authors.lastname: Bielinska
authors.forename: L
authors.initials: L
authors.lastname: Baier
authors.forename: N
authors.initials: N
authors.lastname: Hailat
authors.forename: J R
authors.initials: JR
authors.lastname: Strahler
authors.forename: G J
authors.initials: GJ
authors.lastname: Nabel
authors.forename: S
authors.initials: S
authors.lastname: Hanash
mesh_terms: D015756:Genes, tat; D006801:Humans; D008213:Lymphocyte Activation; D009687:Nuclear Proteins / Q000032:analysis*; D010750:Phosphoproteins / Q000032:analysis; D013601:T-Lymphocytes / Q000737:chemistry* / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014162:Transfection
chemical_list: D009687:Nuclear Proteins; D010750:Phosphoproteins; D013755:Tetradecanoylphorbol Acetate