Introduction of single-stranded ADH genes into Drosophila results in tissue-specific expression.
We injected single-stranded circular DNA containing a Drosophila Adh gene into ADH-negative embryos of Drosophila melanogaster and performed ADH histochemical staining on third instar larvae of the injected generation. Introduction of either the coding or non-coding strand resulted in correct tissue-specific expression of the Adh gene in larvae. Southern blotting revealed that the bulk of the injected DNA became double-stranded shortly after injection and was not integrated into the genome.
Shen
N L
NL
Waksman Institute, Rutgers-State University of New Jersey, Piscataway 08855.
Sofer
W H
WH
D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004277:DNA, Single-Stranded / Q000008:administration & dosage / Q000235:genetics*; D004330:Drosophila / Q000235:genetics*; D015870:Gene Expression; D008845:Microinjections; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010957:Plasmids; D015183:Restriction Mapping; D016385:TATA Box
D004277:DNA, Single-Stranded; D000426:Alcohol Dehydrogenase