title: Preliminary crystallographic study of a pseudoazurin from methylotrophic bacterium, Methylobacterium extorquens AM1.
abstract: Single crystals of pseudoazurin, one of the blue copper proteins produced by methylotrophic bacterium Methylobacterium extorquens AM1, have been obtained by the method of vapor diffusion with ammonium sulfate as a precipitant at pH 8.0. Crystals belong to the orthorhombic system, space group P2(1)2(1)2(1), with unit cell dimensions of a = 52.619(4) A, b = 63.280(6) A, c = 35.133(4) A. The asymmetric unit includes one molecule of pseudoazurin (Vm = 2.18 A3/dalton). The crystals are so stable against X-ray irradiation that diffraction intensities of the native crystal up to 1.68 A resolution could be collected from only one crystal. Among the many heavy-metal reagents examined, uranyl acetate gave an effective isomorphous derivative.
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering Osaka University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Inoue
authors.forename: Y
authors.initials: Y
authors.lastname: Kai
authors.forename: S
authors.initials: S
authors.lastname: Harada
authors.forename: N
authors.initials: N
authors.lastname: Kasai
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Kohzuma
authors.forename: J
authors.initials: J
authors.lastname: Tobari
mesh_terms: D001400:Azurin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D006088:Gram-Negative Aerobic Bacteria / Q000378:metabolism*; D011487:Protein Conformation; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: C047163:pseudoazurin; D001400:Azurin