Tyrosine hydroxylase mRNA concentration in midbrain dopaminergic neurons is differentially regulated by reserpine.
Tyrosine hydroxylase (TH)-mRNA, assayed by in situ hybridization combined with TH immunocytochemistry, showed a selective increase in the ventral tegmental area (A-10) but not in the substantia nigra (A-9) midbrain dopaminergic (DAergic) neurons 3 days after reserpine treatment. TH-mRNA in locus ceruleus noradrenergic (A-4) neurons was increased by reserpine, as confirmed by RNA blot hybridization. These findings show that TH-mRNA is differentially regulated in midbrain DAergic neurons in response to reserpine.
Pasinetti
G M
GM
Andrus Gerontology Center, University of Southern California, Los Angeles 90089-0191.
Morgan
D G
DG
Johnson
S A
SA
Millar
S L
SL
Finch
C E
CE
D000818:Animals; D004210:Dissection / Q000379:methods; D004298:Dopamine / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D008636:Mesencephalon / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D009994:Osmolar Concentration; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D012110:Reserpine / Q000494:pharmacology*; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics* / Q000378:metabolism
D012333:RNA, Messenger; D012110:Reserpine; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine