Genetics and biology of human ovarian teratomas. II. Molecular analysis of origin of nondisjunction and gene-centromere mapping of chromosome I markers.
Chromosomal heteromorphisms and DNA polymorphisms have been utilized to identify the mechanisms that lead to formation of human ovarian teratomas and to construct a gene-centromere map of chromosome 1 by using those teratomas that arise by meiotic nondisjunction. Of 61 genetically informative ovarian teratomas, 21.3% arose by nondisjunction at meiosis I, and 39.3% arose by meiosis II nondisjunction. Eight polymorphic marker loci on chromosome 1p and one marker on 1q were used to estimate a gene-centromere map. The results show clear linkage of the most proximal 1p marker (NRAS) and the most proximal 1q marker (D1S61) to the centromere at a distance of 14 cM and 20 cM, respectively. Estimated gene-centromere distances suggest that, while recombination occurs normally in ovarian teratomas arising by meiosis II errors, ovarian teratomas arising by meiosis I nondisjunction have altered patterns of recombination. Furthermore, the estimated map demonstrates clear evidence of chiasma interference. Our results suggest that ovarian teratomas can provide a rapid method for mapping genes relative to the centromere.
Deka
R
R
Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, PA 15261.
Chakravarti
A
A
Surti
U
U
Hauselman
E
E
Reefer
J
J
Majumder
P P
PP
Ferrell
R E
RE
D000293:Adolescent; D000328:Adult; D000368:Aged; D002503:Centromere; D002648:Child; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D002882:Chromosomes, Human, Pair 13; D002891:Chromosomes, Human, Pair 21; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D006801:Humans; D008875:Middle Aged; D009630:Nondisjunction, Genetic; D010051:Ovarian Neoplasms / Q000209:etiology / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D013724:Teratoma / Q000209:etiology / Q000235:genetics*
D015342:DNA Probes; D004273:DNA, Neoplasm; D005819:Genetic Markers