Semi-automated fluorometric assay for urinary total and B N-acetyl-beta-D-glucosaminidase: analytical investigation.
Total N-acetyl-beta-D-glucosaminidase (NAG) and NAG-B isoenzyme determination are determined in order to investigate renal tubular function. Here, we propose a semi-automated adaptation of Sandman's manual method for the Monarch centrifugal analyzer. Fluorescence of the released 4-methylumbelliferone is automatically read and results are directly expressed in nanokatal/l. Mean (1 SD) values expressed in nanokatal/millimole creatinine in urine from healthy female (n = 30) and male (n = 30) subjects were 8.2 (4.0) and 7.8 (2.9) for total NAG and 1.9 (1.4) and 1.5 (0.7) for the NAG-B isoenzyme activity. The assay of six patients with various renal disorders shows definite increase in total NAG and NAG-B isoenzyme activities.
Kristz
W
W
Service de Biochimie-Hormonologie, Hôpital Robert Debre, Paris, France.
Samaan
G J
GJ
Leberre
C
C
Demelier
J F
JF
Biou
D
D
D000118:Acetylglucosaminidase / Q000652:urine*; D000328:Adult; D005069:Evaluation Studies as Topic; D005260:Female; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D007527:Isoenzymes / Q000652:urine*; D007674:Kidney Diseases / Q000201:enzymology; D008297:Male; D012016:Reference Values
D007527:Isoenzymes; D000118:Acetylglucosaminidase