Rapid assessment of haemophilia A carrier state by non-invasive techniques using the polymerase chain reaction.
The polymerase chain reaction (PCR) was used to amplify two polymorphic regions in the factor VIII gene. In vitro synthesis of DNA was achieved using samples obtained from buccal cells, urine, and hair follicles in addition to purified genomic and crude DNA samples prepared from whole blood. Female members of two kindreds affected with haemophilia A were assessed for carrier state using direct restriction fragment length polymorphism analysis of amplified gene products in the Bc1I and XbaI regions. It is concluded that this is a non-invasive, rapid, and inexpensive technique for carrier detection.
Ball
J
J
University Department of Haematology, Royal Hallamshire Hospital.
Warnock
L J
LJ
Preston
F E
FE
D002353:Carrier State / Q000175:diagnosis*; D004247:DNA; D005169:Factor VIII / Q000235:genetics; D005260:Female; D005784:Gene Amplification; D006580:Genetic Carrier Screening / Q000379:methods; D006467:Hemophilia A / Q000235:genetics*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D005169:Factor VIII; D004247:DNA