Activation of protein kinase C by presynaptic FLRFamide receptors facilitates transmitter release at an aplysia cholinergic synapse.
Modulation of evoked quantal transmitter release by protein kinase C (PKC) was investigated at an identified cholinergic neuro-neuronal synapse of the Aplysia buccal ganglion. Evoked acetylcholine release was increased by a diacylglycerol analog that activates PKC and was decreased by H-7, a blocker of PKC. FLRFamide facilitated evoked quantal release by increasing presynaptic Ca2+ influx. The inhibition of PKC by H-7 prevented both the increase of presynaptic Ca2+ influx and the facilitation of evoked acetylcholine release induced by the activation of presynaptic FLRFamide receptors. These results provide evidence that the activation of PKC could be a step in the intracellular pathway by which FLRFamide receptors increase evoked quantal acetylcholine release.
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Laboratoire de Neurobiologie Cellulaire et Moléculaire, CNRS, Gif-sur-Yvette, France.
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Tauc
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D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D001048:Aplysia; D002118:Calcium / Q000502:physiology; D004594:Electrophysiology; D004789:Enzyme Activation; D007546:Isoquinolines / Q000494:pharmacology; D018377:Neurotransmitter Agents / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism* / Q000494:pharmacology; D010275:Parasympathetic Nervous System / Q000378:metabolism* / Q000648:ultrastructure; D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D011978:Receptors, Nicotinic / Q000378:metabolism; D013569:Synapses / Q000378:metabolism*
D007546:Isoquinolines; D018377:Neurotransmitter Agents; D009842:Oligopeptides; D010879:Piperazines; D017981:Receptors, Neurotransmitter; D011978:Receptors, Nicotinic; C066148:phenylalanyl-leucyl-arginyl phenylalaninamide; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011493:Protein Kinase C; D000109:Acetylcholine; D002118:Calcium