Caffeine-induced reduction of the survival of gamma-irradiated HeLa cells and the reversal of the caffeine effect by Escherichia coli RecA protein.
It is confirmed that survival of gamma-irradiated HeLa cells is decreased by post-treatment with caffeine. The caffeine effect is believed to be the result of an inhibition of the repair of gamma-ray-induced DNA damage. In this work we show that the caffeine-induced reduction of the survival of gamma-irradiated HeLa cells is reversed when Escherichia coli RecA protein is introduced into the cells with the aid of liposomes.
Spivak
I M
IM
Institute of Cytology, Academy of Sciences, U.S.S.R., Leningrad.
Kostetsky
I E
IE
Shpielevaya
S P
SP
Kordyum
V A
VA
Zhestyanikov
V D
VD
D002110:Caffeine / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects; D004247:DNA / Q000187:drug effects* / Q000528:radiation effects; D004260:DNA Repair / Q000187:drug effects; D004337:Drug Carriers; D005720:Gamma Rays / Q000009:adverse effects; D006367:HeLa Cells; D006801:Humans; D011938:Rec A Recombinases / Q000494:pharmacology*
D004337:Drug Carriers; D002110:Caffeine; D004247:DNA; D011938:Rec A Recombinases