Cloning and sequencing of Lol pI, the major allergenic protein of rye-grass pollen.
We have isolated a full length cDNA clone encoding the major glycoprotein allergen Lol pI. The clone was selected using a combination of immunological screening of a cDNA expression library and PCR amplification of Lol pI-specific transcripts. Lol pI expressed in bacteria as a fusion protein shows recognition by specific IgE antibodies present in sera of grass pollen-allergic subjects. Northern analysis has shown that the Lol pI transcripts are expressed only in pollen of rye-grass. Molecular cloning of Lol pI provides a molecular genetic approach to study the structure-function relationship of allergens.
Griffith
I J
IJ
ImmuLogic Pharmaceutical Corporation, Cambridge, MA 02139.
Smith
P M
PM
Pollock
J
J
Theerakulpisut
P
P
Avjioglu
A
A
Davies
S
S
Hough
T
T
Singh
M B
MB
Simpson
R J
RJ
Ward
L D
LD
D000485:Allergens; D000595:Amino Acid Sequence; D052179:Antigens, Plant; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant; D006023:Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins; D011058:Pollen / Q000235:genetics*; D012434:Secale / Q000235:genetics* / Q000276:immunology
D000485:Allergens; D052179:Antigens, Plant; D006023:Glycoproteins; C042814:Lol p I protein, Lolium perenne; D010940:Plant Proteins; D004247:DNA