Pharmacological modulation of 2-methyl-carbamate-PAF induced rat paw oedema.
Intraplantar injections of 2-methyl-carbamate-PAF (2-MC) (0.125-16.0 micrograms/paw) into the rat paw were followed by a bell-shaped dose response curve for inflammatory oedema, with an ascending phase at 0.125-2.0 micrograms/paw, and a descending phase at 4.0-16.0 micrograms/paw. The inflammatory response to 2-MC was partially inhibited by pre-treatment with aspirin (200 mg kg-1), NDGA (100 mg kg-1), dexamethasone (0.1 mg kg-1), verapamil (50 mg kg-1) and by a specific PAF antagonist BN 52021 (5-10 mg kg-1). The cyclo-oxygenase inhibitors indomethacin (2 mg kg-1) and piroxicam (1.8 mg kg-1) as well as antihistamine meclizine (40 mg kg-1) and ranitidine (50 mg kg-1) failed to block the oedematogenic response to 2-MC. Our data suggest that 2-MC induced rat paw oedema is mediated by PAF-acether receptors and is partially dependent on arachidonate lipoxygenase pathway and extracellular Ca2+.
Castro-Faria-Neto
H C
HC
Departamento de Fisiologia e Farmacodinâmica, Fundação, Oswaldo Cruz, Rio de Janeiro, Brazil.
Silva
P M
PM
Martins
M A
MA
Silva
P S
PS
Henriques
M G
MG
Cordeiro
R S
RS
Vargaftig
B B
BB
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002219:Carbamates / Q000494:pharmacology*; D004224:Diterpenes; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy*; D005260:Female; D046934:Ginkgolides; D007783:Lactones / Q000494:pharmacology; D008297:Male; D010972:Platelet Activating Factor / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
C050584:2-methyl carbamate-platelet activating factor; D000894:Anti-Inflammatory Agents, Non-Steroidal; D002219:Carbamates; D004224:Diterpenes; D046934:Ginkgolides; D007783:Lactones; D010972:Platelet Activating Factor; C045856:ginkgolide B