Restriction fragment length polymorphism analysis of the L-myc gene locus in a case-control study of lung cancer.
The L-myc DNA-restriction fragment length polymorphism, revealed by EcoRI, has been studied in both a lung cancer case-control framework and a cohort of 40 non-diseased unrelated individuals. No association was found between the L-myc allelic frequencies and disease status, tumor stage or lung cancer histology. A strong association was, however, observed between the L-myc allelic frequencies and ethnic origin (black or white) of the subjects. Among American whites the allelic distribution at the L-myc proto-oncogene locus was almost identical to that previously reported for Japanese subjects. Among the American black population there was a significantly higher frequency of the presence of the polymorphic EcoRI restriction site in the second intron of the L-myc proto-oncogene. These data emphasize the importance of conducting epidemiologic studies that control for ethnic factors and indicate that L-myc EcoRI allelotypes do not appear to be predictive of lung cancer risk or disease status in American blacks and whites.
Tamai
S
S
Laboratory of Human Carcinogenesis, National Cancer Institute, NIH, Bethesda, MD 20892.
Sugimura
H
H
Caporaso
N E
NE
Resau
J H
JH
Trump
B F
BF
Weston
A
A
Harris
C C
CC
D000483:Alleles; D000704:Analysis of Variance; D001483:Base Sequence; D001665:Binding Sites; D015139:Blotting, Southern; D016022:Case-Control Studies; D005006:Ethnicity; D006801:Humans; D008175:Lung Neoplasms / Q000208:ethnology / Q000235:genetics*; D008969:Molecular Sequence Data; D009857:Oncogenes; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D000090063:Proto-Oncogene Mas; D015183:Restriction Mapping; D012306:Risk; D014481:United States