Evidence of 5'-terminal modification in the kemerovo virus double-stranded RNA segments and its removal by treatment with alkaline phosphatase.
Only one strand of each double-stranded (ds) RNA segment of the Kemerovo virus genome was 5'end-labelled using gamma-32P-ATP and T 4 polynucleotide kinase after preceding dephosphorylation of 5'ends by calf intestinal alkaline phosphatase. This suggests a 5'-terminal modification of the one of complementary strands in the ds RNA segments.
Bacík
I
I
Institute of Virology, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel; D018163:Orbivirus / Q000235:genetics*; D010766:Phosphorylation; D012315:RNA Caps / Q000032:analysis*; D012330:RNA, Double-Stranded / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D012087:Reoviridae / Q000235:genetics*
D012315:RNA Caps; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D000255:Adenosine Triphosphate; D000469:Alkaline Phosphatase