Saturating the region of the polycystic kidney disease gene with NotI linking clones.
A NotI-linking library was constructed from a radiation hybrid containing fragments of human chromosome 16. The clones were mapped on a panel of somatic cell hybrids, and 10 different NotI site-containing clones were localized close to and between genetic markers flanking the PKD1 locus. With pulsed-field gel analysis the clones were shown to be distributed over four adjacent ClaI fragments covering 1,200 kb.
Himmelbauer
H
H
Imperial Cancer Research Fund, Lincoln's Inn Fields, London, England.
Germino
G G
GG
Ceccherini
I
I
Romeo
G
G
Reeders
S T
ST
Frischauf
A M
AM
D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D002885:Chromosomes, Human, Pair 16 / Q000528:radiation effects; D002999:Clone Cells; D004247:DNA / Q000235:genetics; D004586:Electrophoresis; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D007690:Polycystic Kidney Diseases / Q000235:genetics*
D004247:DNA