Platelet storage in synthetic media lacking glucose and bicarbonate.
The goal of these studies was to develop a cost-effective medium for the storage at 22 +/- 2 degrees C of platelets for transfusion. Platelet concentrates were prepared from platelet-rich plasma in a standard fashion and resuspended in 55 mL of medium. Residual plasma was 14.4 +/- 4.3 percent of the final suspending solution. The use of a simple solution containing electrolytes and citrate, but no glucose or bicarbonate, was associated with a drop in pH to the range of 6.0 to 6.6, as a result of the production of lactic acid from the platelet glycogen and glucose present in residual plasma. When 25 mM (25 mmol/L) sodium phosphate was included as a buffer, the drop in pH was retarded, but the range throughout storage was still 6.5 to 6.8. Nonetheless, platelets stored for 5 days in this phosphate-containing medium showed satisfactory maintenance of many in vitro variables. However, there was a 10 to 35 percent reduction of in vivo recovery after isotopic labeling. There was no significant reduction in subsequent mean cell life. Because of the in vivo abnormalities, these media are not recommended for use at the present time. However, the results are encouraging and suggest that further research may lead to a satisfactory, cost-effective medium.
Murphy
S
S
Cardeza Foundation for Hematologic Research, Philadelphia, Pennsylvania.
Kagen
L
L
Holme
S
S
Gottlieb
B
B
Heaton
W
W
Grode
G
G
Davisson
W
W
Buchholz
D
D
D000255:Adenosine Triphosphate / Q000097:blood; D001639:Bicarbonates; D001792:Blood Platelets / Q000502:physiology*; D001793:Blood Preservation / Q000379:methods*; D002021:Buffers; D002470:Cell Survival; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005947:Glucose; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000097:blood; D019344:Lactic Acid; D010710:Phosphates; D010974:Platelet Aggregation; D013917:Thrombin / Q000494:pharmacology
D001639:Bicarbonates; D002021:Buffers; D005230:Fatty Acids, Nonesterified; D007773:Lactates; D010710:Phosphates; D019344:Lactic Acid; D000255:Adenosine Triphosphate; D013917:Thrombin; D005947:Glucose; C018279:sodium phosphate