title: A serum heterodimer from hagfish (Eptatretus stoutii) exhibits structural similarity and partial sequence identity with immunoglobulin.
abstract: We have isolated, characterized, and partially sequenced immunoglobulin from the most primitive extant nonvertebrate craniate, the hagfish, a jawless fish that may have diverged from the vertebrate lineage more than 500 million years ago. The 160-kDa protein, which is a minor serum component, is composed of two different heavy chains of 69 and 74 kDa and a light chain of 29 kDa and resembles known immunoglobulin on the basis of an equimolar ratio of heavy and light chains, N-linked glycosylation of heavy chains, presence of intra- and interchain disulfide bonds, and polydispersity of each peptide chain. High molecular mass (polymeric) as well as low molecular mass (monomeric) forms were isolated from serum. The hagfish immunoglobulin is unique in that each heterodimer is composed of two different heavy chains and two light chains. The partial peptide maps and amino acid compositions of the two heavy chains differ; the chains do not crossreact immunologically. Slight crossreactivity of the 74-kDa heavy chain with antisera against purified shark immunoglobulin and some conservation of amino acid sequences, including those surrounding a cysteine, suggest that the isolated protein is an immunoglobulin.
authors.affiliation: Department of Pediatrics, University of South Florida, All Children's Hospital, St. Petersburg 33701.
authors.forename: J
authors.initials: J
authors.lastname: Varner
authors.forename: P
authors.initials: P
authors.lastname: Neame
authors.forename: G W
authors.initials: GW
authors.lastname: Litman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001798:Blood Proteins / Q000235:genetics* / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006196:Hagfishes / Q000276:immunology*; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics*; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001798:Blood Proteins; D007136:Immunoglobulins; D046911:Macromolecular Substances