Direct mitogenic properties of interleukin-1.
Optimal lymphocyte activation generally requires two concurrent signals, one involving the T cell receptor, and another supplied by an accessory cytokine such as interleukin-1. We investigated the conditions under which partial cellular activation occurs in the absence of signal to the T cell receptor. Both murine thymocytes and the T cell clone D10.G4.1 responded to recombinant interleukin-1 in the absence of a mitogenic signal, although the magnitude of these responses was smaller and required higher concentrations of interleukin-1 than if a comitogen had been present. Interleukin-1 and interleukin-6 in combination were synergistic and induced tritiated thymidine incorporation in D10.G4.1 cells equal to 15% of that obtained with optimal concentrations of concanavalin A. Such synergy suggests that a significant degree of nonspecific activation of lymphocytes may occur in the presence of combinations of monocyte-derived cytokines.
Wallis
R S
RS
Case Western Reserve University, Cleveland, Ohio.
Fayen
J
J
Wiblin
T S
TS
Fujiwara
H
H
Ellner
J J
JJ
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology; D004357:Drug Synergism; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D051379:Mice; D008934:Mitogens; D011994:Recombinant Proteins / Q000494:pharmacology; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects
D007375:Interleukin-1; D015850:Interleukin-6; D008934:Mitogens; D011994:Recombinant Proteins; D003208:Concanavalin A