Electrophysiological and pharmacological properties of single spinal neurons isolated from adult bullfrogs.
1. We developed an isolated spinal cell preparation from adult bullfrogs. 2. The average resting membrane potential was -60 mV, and an action potential was activated by positive current injection. 3. The cells retained their tetrodotoxin-sensitive Na+ channels and at least two kinetically different types of K+ channel. 4. Under K(+)-free conditions, responses to GABA were blocked by bicuculline while responses to glycine, taurine or beta-alanine were blocked by strychnine. 5. The potency of excitatory amino acids decreased in the order: kainic acid greater than glutamate greater than NMDA. 6. These studies demonstrated that the isolated cells are applicable for electrophysiological and pharmacological investigations.
Adachi
S
S
Department of Toxicology and Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Oka
J
J
Fukuda
H
H
D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D008564:Membrane Potentials / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D018377:Neurotransmitter Agents / Q000494:pharmacology; D011892:Rana catesbeiana / Q000033:anatomy & histology* / Q000502:physiology; D013116:Spinal Cord / Q000166:cytology* / Q000502:physiology
D000596:Amino Acids; D018377:Neurotransmitter Agents