Induction of in vitro differentiation of mouse erythroleukemia cells by genistein, an inhibitor of tyrosine protein kinases.
We have found that genistein, a specific inhibitor of tyrosine protein kinases, induces in vitro erythroid differentiation of mouse erythroleukemia cells. Characterization of the induction process indicated that the genistein-induced differentiation is different from that induced by conventional inducing agents such as dimethyl sulfoxide or hexamethylene-bisacetamide. This conclusion was based upon the earlier appearance of differentiated cells, insensitivity to a specific inhibitor (dexamethasone), and responsiveness of some of the differentiation-resistant cells to genistein in the genistein-induced erythroid differentiation. Possible biological significance of this finding is discussed with respect to the involvement of protein phosphorylation (or dephosphorylation) in mouse erythroleukemia cell differentiation.
Watanabe
T
T
Institute of Applied Microbiology, University of Tokyo, Japan.
Kondo
K
K
Oishi
M
M
D000081:Acetamides; D000818:Animals; D001560:Benzidines / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology; D004121:Dimethyl Sulfoxide; D019833:Genistein; D006454:Hemoglobins / Q000378:metabolism; D007529:Isoflavones / Q000494:pharmacology*; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000081:Acetamides; D001560:Benzidines; D006454:Hemoglobins; D007529:Isoflavones; C029876:benzidine; D003907:Dexamethasone; D019833:Genistein; D011505:Protein-Tyrosine Kinases; C014026:hexamethylene bisacetamide; D004121:Dimethyl Sulfoxide