A chicken beta-actin gene can complement a disruption of the Saccharomyces cerevisiae ACT1 gene.
Recently it was demonstrated that beta-actin can be produced in Saccharomyces cerevisiae by using the expression plasmid pY beta actin (R. Karlsson, Gene 68:249-258, 1988), and several site-specific mutants are now being produced in a protein engineering study. To establish a system with which recombinant actin mutants can be tested in vivo and thus enable a correlation to be made with functional effects observed in vitro, a yeast strain lacking endogenous yeast actin and expressing exclusively beta-actin was constructed. This strain is viable but has an altered morphology and a slow-growth phenotype and is temperature sensitive to the point of lethality at 37 degrees C.
Karlsson
R
R
Department of Zoological Cell Biology, University of Stockholm, Sweden.
Aspenström
P
P
Byström
A S
AS
D000199:Actins / Q000737:chemistry / Q000235:genetics*; D000818:Animals; D015139:Blotting, Southern; D002645:Chickens / Q000235:genetics*; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D005816:Genetic Complementation Test; D007526:Isoelectric Point; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*
D000199:Actins