title: T-lymphocyte interleukin 2-dependent tyrosine protein kinase signal transduction involves the activation of p56lck.
abstract: Addition of interleukin 2 (IL-2) to IL-2-dependent T cells results in tyrosine protein kinase signal transduction events even though the IL-2 receptor alpha and beta chains lack intrinsic enzymatic activity. Here we report that addition of IL-2 to IL-2-dependent human T cells transiently stimulates the specific activity of p56lck, a member of the src family of nonreceptor tyrosine protein kinases expressed at high levels in T lymphocytes. The ability of IL-2 to induce p56lck activation was found to be independent of the capacity of p56lck to associate with either CD4 or CD8. Following IL-2 treatment, p56lck was found to undergo serine/threonine phosphorylation modifications that resulted in altered mobility of the lck gene product on polyacrylamide gels. These observations raise the possibility that p56lck participates in IL-2-mediated signal transduction events in T cells.
authors.affiliation: Laboratory of Tumor Virus Biology, National Cancer Institute, Bethesda, MD 20892.
authors.forename: I D
authors.initials: ID
authors.lastname: Horak
authors.forename: R E
authors.initials: RE
authors.lastname: Gress
authors.forename: P J
authors.initials: PJ
authors.lastname: Lucas
authors.forename: E M
authors.initials: EM
authors.lastname: Horak
authors.forename: T A
authors.initials: TA
authors.lastname: Waldmann
authors.forename: J B
authors.initials: JB
authors.lastname: Bolen
mesh_terms: D015704:CD4 Antigens / Q000032:analysis; D002460:Cell Line; D002999:Clone Cells; D004789:Enzyme Activation; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D007700:Kinetics; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000187:drug effects / Q000201:enzymology / Q000276:immunology*
chemical_list: D015704:CD4 Antigens; D007376:Interleukin-2; D011505:Protein-Tyrosine Kinases; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck)