Peripheral blood lymphocyte subpopulations in Indian women with cervical intraepithelial neoplasia and invasive cancer--an immunocytochemical study using monoclonal antibodies.
The peripheral blood lymphocyte subpopulations in Indian women with various grades of cervical intraepithelial neoplasia [33], invasive cancer [22] and matched controls [30] were studied using specific monoclonal antibodies and biotin avidin immunofluorescence technique. Our results showed a significant fall in peripheral T (CD2) B(CD22) cells in patients with CIN III and invasive cervical cancer lesions compared to controls (P less than 0.001). Similarly the quantum of T helper cells (CD4) decreased according to the severity of cervical lesions (P less than 0.01 - P less than 0.001), whereas the T suppressor cells (CD8) depicted an increasing trend in patients with higher grade of cervical lesions (CIN III and invasive cancer, P less than 0.001) as compared to controls. Further, the CD4/CD8 ratio showed a significant downfall with the severity of cervical lesions (P less than 0.01 - P less than 0.001), indicating a perturbance in the homeostasis of host cellular immunity.
Jain
R
R
Division of Immunology and Virology, Maulana Azad Medical College, New Delhi, India.
Gupta
M M
MM
Parashari
A
A
Kaur
S
S
Luthra
U K
UK
D000328:Adult; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D018801:CD2 Antigens; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens; D015815:Cell Adhesion Molecules; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007194:India / Q000208:ethnology; D037102:Lectins; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000276:immunology; D011971:Receptors, Immunologic / Q000032:analysis; D051918:Sialic Acid Binding Ig-like Lectin 2; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D002583:Uterine Cervical Neoplasms / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; C497867:CD22 protein, human; D015704:CD4 Antigens; D016827:CD8 Antigens; D015815:Cell Adhesion Molecules; D037102:Lectins; D011971:Receptors, Immunologic; D051918:Sialic Acid Binding Ig-like Lectin 2