Independent and cooperative behavior of Thy1 and CD3:TcR T cell signalling pathways.
Normal resting spleen T lymphocytes from mice were stimulated in vitro by monoclonal antibodies (mAbs) against either Thy 1 or CD3:TcR surface protein molecules. Although both mAbs were mitogenic, anti-Thy 1 activation generated 5 times more IL2 secretion than anti-CD3 activation under similar conditions. Production of IL3-like activity was comparable for both Thy1 and CD3-mediated activation. In addition, non-mitogenic doses of anti-CD3 and anti-Thy1 (0.16 micrograms/ml and 0.0125% ascites, respectively) mAbs induced T cell activation when provided together. These results indicate that Thy1 signalling cooperates with the CD3:TcR pathway to activate T cells. However, the Thy1 pathway is also regulated independently since IL2 production is larger when stimulated by anti-Thy1 than anti-CD3 mAbs.
Leal
L M
LM
Instituto Nacional do Câncer (INCa), Universidade Federal do Rio de Janeiro, Brasil.
Dosreis
G A
GA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000502:physiology*; D000954:Antigens, Surface / Q000502:physiology*; D017252:CD3 Complex; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000096:biosynthesis; D007377:Interleukin-3 / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D011948:Receptors, Antigen, T-Cell / Q000502:physiology*; D018800:Thy-1 Antigens
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D017252:CD3 Complex; D007376:Interleukin-2; D007377:Interleukin-3; D011948:Receptors, Antigen, T-Cell; D018800:Thy-1 Antigens