Identification of sulfurtransferase enzymes in Azotobacter vinelandii.
Rhodanese and 3-mercaptopyruvate sulphurtransferase have been identified in A. vinelandii. Two distinct active fractions of the two sulphur transferases were obtained after FPLC ion-exchange chromatography of material partially purified from crude extracts. Rhodanese has been purified to homogeneity, and it consists of one polypeptide chain of Mr ca 25,000. A partial purification of 3-mercaptopyruvate sulphurtransferase was obtained.
Pagani
S
S
Dipartimento di Scienze Molecolari Agroalimentari, University of Milan, Italy.
Franchi
E
E
Colnaghi
R
R
Kennedy
C
C
D001395:Azotobacter / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D009584:Nitrogen / Q000378:metabolism; D013466:Sulfurtransferases / Q000378:metabolism*; D013884:Thiosulfate Sulfurtransferase / Q000378:metabolism*
D013466:Sulfurtransferases; D013884:Thiosulfate Sulfurtransferase; C021550:3-mercaptopyruvate sulphurtransferase; D009584:Nitrogen