Effect of caffeine on the expression of a major X-ray induced protein in human tumor cells.
We have examined the effect of caffeine on the concomitant processes of the repair of potentially lethal damage (PLD) and the synthesis of X-ray-induced proteins in the human malignant melanoma cell line, Ul-Mel. Caffeine administered at a dose of 5mM after X radiation not only inhibited PLD repair but also markedly reduced the level of XIP269, a major X-ray-induced protein whose expression has been shown to correlate with the capacity to repair PLD. The expression of the vast majority of other cellular proteins, including seven other X-ray-induced proteins, remained unchanged following caffeine treatment. A possible role for XIP269 in cell cycle delay following DNA damage by X irradiation is discussed.
Hughes
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Department of Radiation Oncology, University of Pennsylvania School of Medicine, Philadelphia 19104.
Boothman
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D002110:Caffeine / Q000494:pharmacology*; D002460:Cell Line; D004260:DNA Repair / Q000187:drug effects; D006801:Humans; D008545:Melanoma / Q000378:metabolism / Q000473:pathology*; D014176:Protein Biosynthesis; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000528:radiation effects
D002110:Caffeine