Purification of prosomatostatin-converting enzymes.
The enzymes responsible for performing cleavage of propeptides at basic amino acids have proven difficult to characterize. Using the processing of anglerfish islet prosomatostatin (PSS) as a model system, we are pursuing the characterization of both a single basic amino acid-specific and a dibasic amino acid-specific converting enzyme. We describe here the model system and protein isolation methods that have allowed significant progress toward complete characterization of the somatostatin-generating propeptide converting enzymes (PCEs).
Mackin
R B
RB
Max Planck Research Program, Salk Institute for Biological Studies, La Jolla, CA.
Noe
B D
BD
Spiess
J
J
D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005399:Fishes; D007515:Islets of Langerhans / Q000201:enzymology; D008954:Models, Biological; D010447:Peptide Hydrolases / Q000302:isolation & purification*; D011498:Protein Precursors / Q000378:metabolism*; D013004:Somatostatin / Q000378:metabolism*
D011498:Protein Precursors; D013004:Somatostatin; C028456:prosomatostatin; D010447:Peptide Hydrolases