title: Stimulation of tracheal ciliary beat frequency by capsaicin.
abstract: To determine the possible involvement of neural and cyclooxygenase pathways whereby irritants might affect cilia activity in vivo, the temporal response of canine tracheal ciliary beat frequency (CBF) to the inhaled surrogate irritant capsaicin was studied. CBF was measured on the ventral midtracheal surface of barbiturate-anesthetized eucapnically ventilated beagle dogs by heterodyne-mode laser light scattering. After base-line CBF was established, hexamethonium bromide (2 mg/kg iv), ipratropium bromide (0.5 microgram/kg iv), indomethacin (2 mg/kg iv), or intravenous 0.9% saline was administered. Aerosolized 3 Z 10(-9) M capsaicin in 0.9% saline was delivered for 2 min, and CBF was measured for the following 60 min. Control experiments used 0.9% saline sham aerosol with a 0.9% saline sham block. Aerosolized capsaicin stimulated CBF from a base line of 6.2 +/- 1.4 (SD) Hz (n = 230) to a mean maximum of 17.7 +/- 7.3 Hz (n = 16) 23 min after aerosol delivery, and CBF returned to base line within 60 min. Neither hexamethonium bromide, ipratropium bromide, nor indomethacin changed CBF from base-line values. The episodic CBF stimulatory response to capsaicin after commencement of aerosol was completely inhibited by hexamethonium bromide. Ipratropium bromide partially inhibited the first 15 min and totally inhibited the following 45 min of stimulatory response. Indomethacin inhibited the initial 15 min but had less effect on the following 45 min of stimulatory response. These data indicate that multiple stimulatory mechanisms function over a prolonged period of time to affect the removal of irritants from the airways and that these mechanisms differ from those involved in the maintenance of basal CBF.
authors.affiliation: Department of Medicine, University of Illinois, Chicago 60680.
authors.forename: L B
authors.initials: LB
authors.lastname: Wong
authors.forename: I F
authors.initials: IF
authors.lastname: Miller
authors.forename: D B
authors.initials: DB
authors.lastname: Yeates
mesh_terms: D000336:Aerosols; D000818:Animals; D002211:Capsaicin / Q000008:administration & dosage / Q000494:pharmacology*; D004285:Dogs; D005260:Female; D018738:Hexamethonium; D006584:Hexamethonium Compounds / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D009241:Ipratropium / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D009079:Mucociliary Clearance / Q000187:drug effects* / Q000502:physiology; D009434:Neural Pathways / Q000187:drug effects / Q000502:physiology; D014132:Trachea / Q000187:drug effects* / Q000294:innervation / Q000502:physiology
chemical_list: D000336:Aerosols; D006584:Hexamethonium Compounds; D018738:Hexamethonium; D009241:Ipratropium; D002211:Capsaicin; D007213:Indomethacin