title: Isolation and characterization of yeast artificial chromosome clones linking the HLA-B and HLA-C loci.
abstract: A 290-kilobase-pair chromosomal segment containing the genes encoding the human class I major histocompatibility complex molecules HLA-B and HLA-C as well as a class I pseudogene has been isolated on three overlapping yeast artificial chromosome (YAC) clones. One YAC clone contains both the HLA-B and HLA-C genes. These loci are located approximately 85 kilobase pairs apart, each in close association with a CpG island. Southern blotting and nucleotide sequencing showed no evidence of alteration of the structure of the cloned DNA in the YACs. End fragments from the YAC inserts have been isolated and used to confirm the overlaps between clones. These fragments can also serve as polymorphic markers for structural analysis of the major histocompatibility complex. Our data show that YAC cloning offers an attractive alternative for analysis of the structures of large gene complexes such as HLA.
authors.affiliation: Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110.
authors.forename: S K
authors.initials: SK
authors.lastname: Bronson
authors.forename: J
authors.initials: J
authors.lastname: Pei
authors.forename: P
authors.initials: P
authors.lastname: Taillon-Miller
authors.forename: M J
authors.initials: MJ
authors.lastname: Chorney
authors.forename: D E
authors.initials: DE
authors.lastname: Geraghty
authors.forename: D D
authors.initials: DD
authors.lastname: Chaplin
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015723:Gene Library; D005805:Genes, MHC Class I; D008040:Genetic Linkage; D015235:HLA-B Antigens / Q000235:genetics*; D015236:HLA-C Antigens / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D015235:HLA-B Antigens; D015236:HLA-C Antigens; D004247:DNA