title: Crystallization and preliminary crystallographic study of cathepsin D inhibitor from potatoes.
abstract: Single crystals of the glycosylated inhibitor of cathepsin D and trypsin isolated from potato tubers were obtained using the hanging drop vapor diffusion method and ammonium nitrate as precipitant. The crystals exhibit strong F222 pseudo symmetry but belong to the orthorhombic space group C222 or C222(1), with cell parameters a = 73.8 A, b = 119.9 A and c = 133.2 A with two molecules per asymmetric unit. The crystals diffract to a resolution of 2.4 A.
authors.affiliation: Laboratory of Molecular Biophysics, University of Oxford, U.K.
authors.forename: M
authors.initials: M
authors.lastname: Baudys
authors.forename: M
authors.initials: M
authors.lastname: Ghosh
authors.forename: K
authors.initials: K
authors.lastname: Harlos
authors.forename: M
authors.initials: M
authors.lastname: Mares
authors.forename: M
authors.initials: M
authors.lastname: Fusek
authors.forename: V
authors.initials: V
authors.lastname: Kostka
authors.forename: C C
authors.initials: CC
authors.lastname: Blake
mesh_terms: D002402:Cathepsin D / Q000037:antagonists & inhibitors*; D003460:Crystallization; D005612:Freeze Drying; D010940:Plant Proteins; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D011198:Solanum tuberosum; D014961:X-Ray Diffraction
chemical_list: D010940:Plant Proteins; D011506:Proteins; C060481:CDI protein, Solanum tuberosum; D002402:Cathepsin D