title: Trypanosoma cruzi: cellular and antibody response against the parasite in mice immunized with a 19-amino acid synthetic peptide.
abstract: Several monoclonal antibodies were prepared against the flagellar fraction of Trypanosoma cruzi epimastigotes (Tulahuén strain, stock Tul 2). One of them, FCH-F8-4, has previously shown biologic activity against the parasite (complement-mediated lysis and neutralization of the trypomastigote infectivity). Immunopurified antigens using this monoclonal antibody elicited a protective immune response in mice. Two recombinant cDNA clones were detected with this anti-flagellar fraction monoclonal antibody on a lambda gt11 expression library prepared from T. cruzi epimastigote mRNA. The insert of one of these cDNA clones, lambda(FCH-F8-4)1 (150 bp) coded for a 19-amino acid peptide (PAFLGCSSRFSGSFSGVEP). This insert hybridized with a 5.0-kb mRNA from epimastigotes. The beta-galactosidase fusion protein was produced in lysogenic bacteria. The monoclonal antibody recognized the epitope present in the fusion protein after western blotting of the crude lysate. A synthetic peptide (SP4) containing the complete sequence of lambda(FCH-F8-4)1 was constructed on solid phase. This peptide was able to inhibit the ELISA reactivity (in a range from 13 to 52%) of flagellar fraction immunized mouse sera and when administered (coupled to KLH or alone) to BALB/c mice with Bordetella pertussis as adjuvant, it induced a humoral and cellular immune response which was detected by ELISA, immunofluorescence, blotting, and DTH reactions against T. cruzi antigens. The immune response obtained indicates that this synthetic peptide resembles the parasite antigen conformation and could be useful for diagnosis purposes or be able to elicit immunoprotection against T. cruzi infection.
authors.affiliation: Instituto Nacional de Diagnóstico e Investigación de la Enfermedad de Chagas Dr. Mario Fatala Chaben, Buenos Aires, Argentina.
authors.forename: J
authors.initials: J
authors.lastname: Búa
authors.forename: E J
authors.initials: EJ
authors.lastname: Bontempi
authors.forename: M
authors.initials: M
authors.lastname: Levin
authors.forename: A
authors.initials: A
authors.lastname: Orn
authors.forename: D
authors.initials: D
authors.lastname: Velasco
authors.forename: M
authors.initials: M
authors.lastname: Moreno
authors.forename: P
authors.initials: P
authors.lastname: Levi-Yeyati
authors.forename: A
authors.initials: A
authors.lastname: Engstrom
authors.forename: E L
authors.initials: EL
authors.lastname: Segura
authors.forename: A M
authors.initials: AM
authors.lastname: Ruiz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D000953:Antigens, Protozoan / Q000138:chemical synthesis / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D016054:DNA, Protozoan / Q000737:chemistry; D006968:Hypersensitivity, Delayed; D007114:Immunization; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010455:Peptides / Q000276:immunology; D015800:Protozoan Proteins / Q000138:chemical synthesis / Q000235:genetics / Q000276:immunology*; D014349:Trypanosoma cruzi / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D010455:Peptides; D015800:Protozoan Proteins