In vivo promoter activity of the synthetic Pribnow box.
The synthetic polydeoxyribonucleotides TGCA(TTATAATGCA)1-4, containing the consensus Pribnow box, were inserted upstream of the promoterless gal operon in a specially constructed plasmid. Using the MacConkey agar indicator technique it has been shown that these inserts are able to induce an efficient in vivo expression of the gal operon.
Koroleva
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ON
A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.
Drutsa
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VL
D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005690:Galactose; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D009838:Oligodeoxyribonucleotides; D012321:DNA-Directed RNA Polymerases; D005690:Galactose