The in vivo blood, fat and muscle concentrations of lignocaine and bupivacaine in the hindquarters of sheep.
1. A method was developed for sampling muscle and fat from the hindquarters of sheep undergoing spinal anaesthesia. The method was used to measure the concentrations of lignocaine and bupivacaine in the blood, muscle and fat of the hindquarters of sheep during and after 180 min constant-rate infusions of the drugs. 2. For both drugs the muscle drug concentrations were a relatively constant ratio of the simultaneous arterial blood drug concentrations during and after the infusion. 3. There was uptake of both lignocaine and bupivacaine into subcutaneous fat during the infusions. At the end of the infusion the ratio of the fat: arterial blood drug concentrations were 1.54 (SD = 0.57, n = 4) and 3.1 (SD = 1.4, n = 4) for lignocaine and bupivacaine, respectively. 4. The drug concentrations in fat declined relatively slowly after the infusion. The ratio of the fat: arterial blood drug concentrations 180 min after the end of the infusion was 21.5 (SD 4.0, n = 3) and for lignocaine, and 120 min after the end of the infusion was 9.54 (SD 5.2, n = 3) for bupivacaine. 5. It was concluded that the concentrations of lignocaine and bupivacaine in muscle were essentially in equilibrium with the arterial concentrations during and after the infusion. However, the concentrations of lignocaine and bupivacaine in fat were not in equilibrium with the arterial concentrations in the post-infusion period.
Upton
R N
RN
Department of Anaesthesia and Intensive Care, Flinders University of South Australia, Bedford Park.
Nancarrow
C
C
McLean
C F
CF
Mather
L E
LE
Runciman
W B
WB
D000273:Adipose Tissue / Q000378:metabolism; D000775:Anesthesia, Spinal; D000818:Animals; D001693:Biological Transport, Active; D002045:Bupivacaine / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D006439:Hemodynamics; D007261:Infusions, Intra-Arterial; D008012:Lidocaine / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D009132:Muscles / Q000378:metabolism; D012756:Sheep; D014018:Tissue Distribution
D008012:Lidocaine; D002045:Bupivacaine