Molecular cloning and sequence analysis of bovine lactotransferrin.
The screening of a bovine submaxillary gland cDNA library yielded 25 clones coding for bovine lactotransferrin. The nucleotide sequence of the longest insert contained a protein-coding region of 2115 nucleotides and a 3' non-coding region of 194 nucleotides followed by a poly(A) tract of about 55 nucleotides. The predicted peptide sequence included a 16-amino-acid signal sequence upstream of the first amino acid of the native protein. The identity of the clone was confirmed by matching the amino acid sequence predicted from the cDNA with the N-terminal and tryptic peptide sequences derived from purified bovine milk lactotransferrin, and also by similarity with human and murine lactotransferrins. The cDNA described corresponds to a 705-amino-acid-long preprotein that lacks the start methionine. The sequence of the secreted protein is 689 amino acids long and contains five potential glycosylation sites. Bovine lactotransferrin is 69% and 64% identical to human and murine lactotransferrins, respectively.
Pierce
A
A
Laboratoire de Chimie Biologique (Unité Mixte de Recherche no. 111 du Centre National de la Recherche Scientifique), Université des Sciences et Techniques de Lille Flandres-Artois, Villeneuve d'Ascq, France.
Colavizza
D
D
Benaissa
M
M
Maes
P
P
Tartar
A
A
Montreuil
J
J
Spik
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D007781:Lactoferrin / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data
D004247:DNA; D007781:Lactoferrin