Mutagenicity and antimutagenicity of air-borne particulates.
A methanol extract of air-borne particulates collected in a suburban area of Okayama City showed not only mutagenicity but also antimutagenicity in the Ames test. Thus, when the mutagenicity of this preparation in Salmonella typhimurium TA98 (with metabolic activation) was measured, we observed that the dose response reached a plateau at 27 m3 air equivalent of the particulate, showing approximately equal numbers of revertants in the dose range 27-270 m3 equivalent. This plateau formation was not seen in the dose response of a blue-cotton extract of this preparation: the extract gave a linearly increasing dose response up to 270 m3 equivalent. This finding suggests that some factors that inhibit the mutagenicity were present in this methanol extract and that these inhibitors were not adsorbable to blue cotton, an adsorbent selective for compounds having 3 or more fused rings. From the portion unadsorbed to blue cotton, we isolated the antimutagenic factors and identified them as long-chain fatty acids: palmitic, stearic, oleic and linoleic acids. Analysis of several samples, including those from other parts of Japan, has suggested that these antimutagenic fatty acids are ubiquitous in air-borne particulates.
Iwado
H
H
Okayama Prefectural Institute for Environmental Science and Public Health, Japan.
Naito
M
M
Hayatsu
H
H
D000393:Air Pollutants / Q000032:analysis / Q000633:toxicity*; D004247:DNA / Q000187:drug effects; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000032:analysis; D016296:Mutagenesis; D009152:Mutagenicity Tests; D019301:Oleic Acid; D009829:Oleic Acids / Q000032:analysis; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000032:analysis; D012486:Salmonella typhimurium / Q000235:genetics; D013229:Stearic Acids / Q000032:analysis
D000393:Air Pollutants; D008041:Linoleic Acids; D009829:Oleic Acids; D010169:Palmitic Acids; D013229:Stearic Acids; D019301:Oleic Acid; D019308:Palmitic Acid; C031183:stearic acid; D004247:DNA; D019787:Linoleic Acid