Gamma-glutamyl transpeptidase and acetylcholinesterase activities in brain capillaries of cholesterol-fed rabbits.
Cerebral microvascular endothelium, the constituent cell of the blood-brain barrier, is enriched in the enzyme gamma-glutamyl transpeptidase (GGT). This enzyme plays a role in the regulation of amino acid uptake and transport, and in the gamma-glutamylation of serotonin, dopamine and norepinephrine. Recent studies have demonstrated that GGT activity is modulated by cholinergic-adrenergic agonists. The levels of the acetylcholine-catabolizing enzyme acetylcholinesterase (AChE), may therefore be related to the modulation of the GGT activity. In this study, the activities of GGT and AChE in microvessel-enriched fractions were assayed after feeding of rabbits a high cholesterol diet. A 21% decrease of GGT activity and a 44% increase of AChE activity appeared at the end of dietary treatment.
Eryürek
F G
FG
Department of Biochemistry, Istanbul Faculty of Medicine, University of Istanbul, Capa, Turkey.
Sürmen
E
E
Oner
P
P
Altuğ
T
T
Oz
H
H
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001161:Arteriosclerosis / Q000201:enzymology; D002196:Capillaries / Q000187:drug effects / Q000201:enzymology*; D002560:Cerebrovascular Circulation / Q000187:drug effects; D002791:Cholesterol, Dietary / Q000494:pharmacology*; D008055:Lipids / Q000097:blood; D008297:Male; D008833:Microcirculation / Q000187:drug effects / Q000201:enzymology; D011817:Rabbits; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
D002791:Cholesterol, Dietary; D008055:Lipids; D005723:gamma-Glutamyltransferase; D000110:Acetylcholinesterase