Prohibitin, an evolutionarily conserved intracellular protein that blocks DNA synthesis in normal fibroblasts and HeLa cells.
Genes that act inside the cell to negatively regulate proliferation are of great interest because of their implications for such processes as development and cancer, but these genes have been difficult to clone. This report details the cloning and analysis of cDNA for prohibitin, a novel mammalian antiproliferative protein. Microinjection of synthetic prohibitin mRNA blocks entry into S phase in both normal fibroblasts and HeLa cells. Microinjection of an antisense oligonucleotide stimulates entry into S phase. By sequence comparison, the prohibitin gene appears to be the mammalian analog of Cc, a Drosophila gene that is vital for normal development.
Nuell
M J
MJ
Laboratory of Molecular Genetics, National Institute on Aging, Baltimore, Maryland 21224.
Stewart
D A
DA
Walker
L
L
Friedman
V
V
Wood
C M
CM
Owens
G A
GA
Smith
J R
JR
Schneider
E L
EL
Dell' Orco
R
R
Lumpkin
C K
CK
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002453:Cell Cycle; D003001:Cloning, Molecular; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D005796:Genes; D006367:HeLa Cells; D006801:Humans; D008099:Liver / Q000502:physiology; D008845:Microinjections; D008969:Molecular Sequence Data; D000091402:Prohibitins; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D012097:Repressor Proteins; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D000091402:Prohibitins; D011506:Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; D004247:DNA