title: In vivo promoter activity of the synthetic Pribnow box.
abstract: The synthetic polydeoxyribonucleotides TGCA(TTATAATGCA)1-4, containing the consensus Pribnow box, were inserted upstream of the promoterless gal operon in a specially constructed plasmid. Using the MacConkey agar indicator technique it has been shown that these inserts are able to induce an efficient in vivo expression of the gal operon.
authors.affiliation: A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.
authors.forename: O N
authors.initials: ON
authors.lastname: Koroleva
authors.forename: V L
authors.initials: VL
authors.lastname: Drutsa
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005690:Galactose; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic
chemical_list: D004269:DNA, Bacterial; D009838:Oligodeoxyribonucleotides; D012321:DNA-Directed RNA Polymerases; D005690:Galactose