Cloning and nucleotide sequence of a bovine pancreatic preprocarboxypeptidase A cDNA.
A full-length cDNA clone encoding bovine pancreatic preprocarboxypeptidase A was isolated and sequenced. The 1405-base pair insert contains a 26-nucleotide 5'-noncoding region, a 1260-nucleotide open reading frame and a 76-nucleotide 3'-noncoding fragment plus a poly(A) tail of at least 43 nucleotides. The open reading frame encodes a protein of 419 amino acids, including the 16 amino acid signal peptide. The mature enzyme (309 residues) has two additional C-terminal amino acids, as compared with the amino acid sequence of the protein which was reported more than 20 years ago. In addition, four residues deduced from the nucleotide sequence differed from those identified in the reported amino acid sequence from their net charge: Asp-89, Asp-114, Gln-122, and Asp-185 instead of Asn-89, Asn-114, Glu-122, and Asn-185, respectively. A high degree of identity exists between the nucleotide sequences (81.3%), on the one hand, and the amino acid sequences (78.3%), on the other hand, of bovine preprocarboxypeptidase A and rat preprocarboxypeptidase A1.
Le Huërou
I
I
Centre de Biochemie et de Biologie Moléculaire, Centre National de la Recherche Scientifique, Marseille, France.
Guilloteau
P
P
Toullec
R
R
Puigserver
A
A
Wicker
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002268:Carboxypeptidases / Q000235:genetics*; D043422:Carboxypeptidases A; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004792:Enzyme Precursors / Q000235:genetics*; D015723:Gene Library; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D004792:Enzyme Precursors; D012333:RNA, Messenger; D004247:DNA; D002268:Carboxypeptidases; D043422:Carboxypeptidases A