title: New method for isolation of venom from the sea anemone Actinia cari. Purification and characterization of cytolytic toxins.
abstract: 1. Venom from the sea anemone Actinia cari was obtained by the "milking" method. Two lethal and hemolytic polypeptide toxins, caritoxins I (CTX I) and II (CTX II), were isolated with gel and ion exchange chromatography. 2. The mol. wt of the pure toxin was 19,800. The isoelectric points of CTX I and II were 9.45 and 10.0, respectively. The toxins had similar amino acid compositions lacking cysteine. 3. The intravenous CTX I and CTX II lethal dose (LD50) in mice was found to be 54 +/- 25 and 90 +/- 1 micrograms/kg, respectively. Their hemolytic activity was inhibited by sphingomyelin.
authors.affiliation: Department of Biology, Biotechnical Faculty, E. Kardelj University of Ljubljana, Yugoslavia.
authors.forename: L
authors.initials: L
authors.lastname: Sencic
authors.forename: P
authors.initials: P
authors.lastname: Macek
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003064:Cnidarian Venoms / Q000008:administration & dosage / Q000302:isolation & purification* / Q000633:toxicity; D006461:Hemolysis; D007526:Isoelectric Point; D007928:Lethal Dose 50; D051379:Mice; D008970:Molecular Weight; D012615:Sea Anemones / Q000032:analysis*; D013109:Sphingomyelins / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D003064:Cnidarian Venoms; D013109:Sphingomyelins