The use of a hapten-Fab conjugate to sensitize target cells for antibody-dependent complement-mediated lysis and antibody-dependent cell-mediated cytotoxicity.
NIP-conjugated Fab' fragments from a rabbit hyperimmunized with sheep red blood cells (SRBC) were used to link the hapten NIP to target cells for antibody-dependent cell-mediated cytotoxicity (ADCC) and antibody-dependent complement-mediated lysis (ADCML). Target cells (SRBC) labelled with this NIP-Fab' complex were compared with SRBC directly haptenized with NIP in ADCC and ADCML assays using a NIP specific IgG1 chimeric antibody. Both methods yielded almost identical results. Using the NIP-FAb' conjugate identical target cell haptenization was readily achieved from experiment to experiment. Using conjugates of different NIP/Fab' ratios it should be possible to study how such changes influence antibody effector functions.
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Department of Immunology, National Institute of Public Health, Oslo, Norway.
Michaelsen
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D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D003167:Complement Activation; D004305:Dose-Response Relationship, Drug; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D000922:Immunotoxins / Q000276:immunology; D066298:In Vitro Techniques; D011817:Rabbits
D007140:Immunoglobulin Fab Fragments; D000922:Immunotoxins