Capillary electrophoresis--a new clinical tool.
The potential clinical diagnostic utility of capillary electrophoresis in an open-tubular column is established. Separation patterns for serum proteins by conventional agarose gel electrophoresis can be faithfully reproduced by a capillary electrophoresis procedure that provides the complete run data in 8 min. Hemoglobin variants can be separated within 10 min. The capillary electrophoresis separations are performed reliably and reproducibly in an untreated 75 micron (i.d.) x 25 cm fused-silica column. Diluted serum or hemoglobin samples can be loaded on an automated instrument with on-line injection, detection, and quantification, providing a truly "walkaway" electrophoresis system.
Chen
F T
FT
Applied Research Department, Beckman Instruments Inc., Brea, CA 92621.
Liu
C M
CM
Hsieh
Y Z
YZ
Sternberg
J C
JC
D001798:Blood Proteins / Q000302:isolation & purification; D002197:Capillary Action; D002556:Cerebrospinal Fluid Proteins / Q000302:isolation & purification; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel; D006455:Hemoglobins, Abnormal / Q000302:isolation & purification; D006801:Humans; D011507:Proteinuria / Q000652:urine
D001798:Blood Proteins; D002556:Cerebrospinal Fluid Proteins; D006455:Hemoglobins, Abnormal