title: Dystrophin is tightly associated with the sarcolemma of mammalian skeletal muscle fibers.
abstract: Sarcolemmal vesicles with right-side-out configuration were prepared from normal fresh human and rabbit skeletal muscle bundles by incubation in 140 mM KCl solution containing collagenase. The vesicles were used to examine the association of dystrophin, the protein product of the Duchenne muscular dystrophy gene, with the sarcolemma. Western blot analysis, indirect immunofluorescence, and immunoperoxidase staining using specific antibodies raised against the N-terminal and the C-terminal domains show that dystrophin remains associated with the membrane of sarcolemmal vesicles. Indirect immunofluorescence microscopy using permeabilized and unpermeabilized vesicles indicated that both the N-terminus and the C-terminus of dystrophin are localized to the cytoplasmic surface of the sarcolemma. These results suggest that dystrophin has much stronger attachment to the surface membrane than it has to the internal domain of skeletal muscle fibers. Sarcolemmal vesicles thus represent a new system for studying the function of dystrophin and the molecular basis of its association with the sarcolemma.
authors.affiliation: Genetics Department, Hospital for Sick Children, Toronto, Ontario, Canada.
authors.forename: E E
authors.initials: EE
authors.lastname: Zubrzycka-Gaarn
authors.forename: O F
authors.initials: OF
authors.lastname: Hutter
authors.forename: G
authors.initials: G
authors.lastname: Karpati
authors.forename: H J
authors.initials: HJ
authors.lastname: Klamut
authors.forename: D E
authors.initials: DE
authors.lastname: Bulman
authors.forename: R S
authors.initials: RS
authors.lastname: Hodges
authors.forename: R G
authors.initials: RG
authors.lastname: Worton
authors.forename: P N
authors.initials: PN
authors.lastname: Ray
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D003593:Cytoplasm / Q000737:chemistry; D016189:Dystrophin / Q000032:analysis*; D015151:Immunoblotting; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D012508:Sarcolemma / Q000737:chemistry*
chemical_list: D016189:Dystrophin; D010446:Peptide Fragments