title: Glutamate dehydrogenase: some properties of the rat brain enzyme from different cellular compartments.
abstract: 1. Differences in the GDH activity of neuronal, glial cells and synaptosomes were detected. 2. The enzyme was measured in both directions: synthesis and degradation of glutamate. 3. Synaptosomes were the region with the highest GDH activity. 4. ADP plays an important role in the regulation of the reaction sense. 5. This effector produced higher activation on the enzyme measured in the direction of glutamate synthesis than in the sense of its degradation. 6. The enhancement produced by ADP was dependent on the enzyme localization. The ADP effect is discussed.
authors.affiliation: Instituto de Bioquimica, Facultad de Farmacia, Ciudad Universitaria, Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Arce
authors.forename: S
authors.initials: S
authors.lastname: Cañadas
authors.forename: M J
authors.initials: MJ
authors.lastname: Oset-Gasque
authors.forename: E
authors.initials: E
authors.lastname: Castro
authors.forename: M P
authors.initials: MP
authors.lastname: González
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000166:cytology / Q000201:enzymology*; D004789:Enzyme Activation / Q000187:drug effects; D005969:Glutamate Dehydrogenase / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D009457:Neuroglia / Q000201:enzymology; D009474:Neurons / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D013574:Synaptosomes / Q000201:enzymology
chemical_list: D005971:Glutamates; D018698:Glutamic Acid; D000244:Adenosine Diphosphate; D005969:Glutamate Dehydrogenase