Attaching and effacing lesions in vivo and adhesion to tissue culture cells of Vero-cytotoxin-producing Escherichia coli belonging to serogroups O5 and O103.
Certain isolates of Escherichia coli from humans and animals with enteric disease attach to enterocytes and cause 'attaching and effacing' (AE) lesions. E. coli strain S22-1, serotype O103:H2, isolated from a child with diarrhoea, contained two plasmids; one of these (pDEP12) hybridized with the CVD419 DNA probe derived from a plasmid found in E. coli O157:H7 and associated with expression of fimbriae and ability to adhere to Intestine 407 cells. Strain S102-9, serotype O5:H-, isolated from a calf with dysentery, contained six plasmids, one of which also hybridized with the CVD419 probe. Loss of pDEP12 coincided with reduced adhesion to HEp-2 or Intestine 407 cells cultured in vitro; reintroduction of this plasmid restored adhesiveness. Loss of the plasmid in strain S102-9 that hybridized with the CVD419 probe did not cause a decrease in adhesion. Accumulations of actin were seen in vitro in the fluorescence actin staining (FAS) test of strains S22-1, S102-9 and their derivatives, irrespective of the plasmid content of these strains or the prevalence of attached bacteria. Strain S22-1 and its plasmidless derivative caused AE lesions of equal severity in experimentally infected gnotobiotic piglets; piglets inoculated with an isolate from a healthy human or pig did not develop these lesions.(ABSTRACT TRUNCATED AT 250 WORDS)
Hall
G A
GA
Agricultural and Food Research Council, Institute for Animal Health, Compton Laboratory, Newbury, Berkshire, UK.
Dorn
C R
CR
Chanter
N
N
Scotland
S M
SM
Smith
H R
HR
Rowe
B
B
D018830:Adhesins, Escherichia coli; D000818:Animals; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D002417:Cattle; D002648:Child; D046508:Culture Techniques; D003603:Cytotoxins / Q000378:metabolism*; D015342:DNA Probes; D003967:Diarrhea / Q000209:etiology / Q000382:microbiology / Q000473:pathology; D004926:Escherichia coli / Q000378:metabolism* / Q000648:ultrastructure; D005856:Germ-Free Life; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D010957:Plasmids; D013552:Swine; D014709:Vero Cells
D018830:Adhesins, Escherichia coli; D001425:Bacterial Outer Membrane Proteins; D003603:Cytotoxins; D015342:DNA Probes