Properties of murine antibodies from different V region families specific for bromelain-treated mouse erythrocytes.
We have analyzed the combining site diversity of murine antibodies reactive with bromelain-treated mouse RBC (BrMRBC) in B10.A mice. Although several VH and V kappa genes are used to generate antibodies of this specificity, two combinations account for more than 80% of the repertoire from the spleen: VH11/V kappa 9 and VH12/V kappa 4. Antibodies representing these two predominant groups were found to correspond to two previously reported distinct cross-reactive Id families accounting for most of the anti-BrMRBC reactivity in several strains of mice. mAb of the VH11/V kappa 9 type bound the haptens trimethyl-ammonium and phosphorylcholine much more avidly (approximately 1000 fold) than did the VH12/V kappa 4 type antibodies. Both of these haptens represent constituents of phosphatidylcholine, which BrMRBC-specific antibodies are reported to bind. Despite this differential reactivity, members of both the VH11/V kappa 9 and VH12/V kappa 4 antibody groups lysed BrMRBC with similar efficiencies. These data suggest that the two major classes of BrMRBC-specific antibodies have at least partially different specificities and imply that the events that lead to their high frequencies in the CD5+ repertoire may result from different selective forces.
Conger
J D
JD
Department of Microbiology and Immunology, Duke University Medical Center, Durham, NC 27710.
Sage
H J
HJ
Kawaguchi
S
S
Corley
R B
RB
D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology; D001483:Base Sequence; D001963:Bromelains; D003429:Cross Reactions; D004912:Erythrocytes / Q000276:immunology; D006825:Hybridomas / Q000378:metabolism; D007130:Immunoglobulin Idiotypes / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D010767:Phosphorylcholine / Q000276:immunology; D000644:Quaternary Ammonium Compounds / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology
D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D007135:Immunoglobulin Variable Region; D000644:Quaternary Ammonium Compounds; D010767:Phosphorylcholine; D012710:Serum Albumin, Bovine; D001963:Bromelains