title: Identification of 'cystic fibrosis protein' as a complex of two calcium-binding proteins present in human cells of myeloid origin.
abstract: Cystic fibrosis protein is a serum protein characterized by a pI close to 8.4 and present with a higher concentration in serum and plasma of cystic fibrosis carriers than in controls. This protein was found immunologically indistinguishable from the cystic fibrosis antigen isolated from granulocytes and presenting a sequence analogous to that of MRP-8, a calcium-binding protein expressed in the myeloid cell lineage. Using antibodies directed against MRP-8 and its closely associated calcium-binding protein, MRP-14, we demonstrate here that cystic fibrosis protein purified from serum is a complex of the two proteins MRP-8 and MRP-14.
authors.affiliation: Groupe de Recherche sur les Glandes Exocrines, Marseille, France.
authors.forename: C
authors.initials: C
authors.lastname: Barthe
authors.forename: C
authors.initials: C
authors.lastname: Figarella
authors.forename: J
authors.initials: J
authors.lastname: Carrère
authors.forename: O
authors.initials: O
authors.lastname: Guy-Crotte
mesh_terms: D001798:Blood Proteins / Q000378:metabolism*; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D040501:Calgranulin A; D003550:Cystic Fibrosis / Q000378:metabolism*; D006098:Granulocytes / Q000378:metabolism; D006801:Humans; D046911:Macromolecular Substances
chemical_list: D001798:Blood Proteins; D002135:Calcium-Binding Proteins; D040501:Calgranulin A; D046911:Macromolecular Substances; C026422:cystic fibrosis serum factor