Attenuated neuropathology by nilvadipine after middle cerebral artery occlusion in rats.
We investigated the effects of nilvadipine, a calcium antagonist, on cerebral ischemia in rats. Under halothane anesthesia, 30 rats had a 3-0 nylon suture introduced through the extracranial internal carotid artery to occlude the left middle cerebral artery. Nilvadipine was dissolved in polyethylene glycol 400. Immediately following occlusion, group 1 rats (n = 10) were treated subcutaneously with vehicle and group 2 and 3 rats were treated with 1.0 (n = 10) and 3.2 (n = 10) mg/kg nilvadipine, respectively. Perfusion fixation was performed 24 hours later, and the histopathologic outcomes were quantified. In group 1 infarct volume was 28.2 +/- 11.4% of the total cerebral volume; in groups 2 and 3 infarct volumes were 25.5 +/- 11.6% (NS) and 13.9 +/- 9.2% (p less than 0.05 different from group 1), respectively. Nilvadipine decreased ischemic neuronal injury in a dose-dependent manner and may be of use in the treatment of cerebral ischemia.
Kawamura
S
S
Department of Surgical Neurology, Research Institute for Brain and Blood Vessels-Akita, Japan.
Shirasawa
M
M
Fukasawa
H
H
Yasui
N
N
D000818:Animals; D001157:Arterial Occlusive Diseases / Q000473:pathology*; D001921:Brain / Q000473:pathology*; D002545:Brain Ischemia / Q000473:pathology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002536:Cerebral Arteries; D002544:Cerebral Infarction / Q000473:pathology; D008297:Male; D009474:Neurons / Q000473:pathology; D009543:Nifedipine / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D002121:Calcium Channel Blockers; C035100:nilvadipine; D009543:Nifedipine