A copia-like element in Pisum demonstrates the uses of dispersed repeated sequences in genetic analysis.
A DNA sequence between two legumin genes in Pisum is a member of the copia-like class of retrotransposons and represents one member of a polymorphic and heterogeneous dispersed repeated sequence family in Pisum. This sequence can be exploited in genetic studies either by RFLP analysis where several markers can be scored together, or the segregation of individual elements can be followed after PCR amplification of specific members.
Lee
D L
DL
AFRC Institute of Plant Research, John Innes Institute, Norwich, UK.
Ellis
T H
TH
Turner
L
L
Hellens
R P
RP
Cleary
W G
WG
D001483:Base Sequence; D004247:DNA; D004251:DNA Transposable Elements; D007887:Fabaceae / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins; D014674:Plant Proteins, Dietary / Q000235:genetics; D010946:Plants, Medicinal; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012190:Retroviridae; D000097793:Legumins
D004247:DNA; D004251:DNA Transposable Elements; D010940:Plant Proteins; D014674:Plant Proteins, Dietary; D000097793:Legumins