Glutamine-synthesizing activity in lungs of fed, starved, acidotic, diabetic, injured and septic rats.
The maximal catalytic activity of glutamine synthetase was measured in lung homogenates of the rat (being 5.46 +/- 0.29 mumol/min per g wet wt. or 31.70 +/- 2.62 nmol/min per mg of protein at 37 degrees C, in fed animals). The activity is similar to that of liver, but 16-fold higher than that in quadriceps muscles. Chronic (NH4Cl-induced) or acute (HCl-induced) metabolic acidosis had no effects on enzyme activity, but there was a marked increase in the activity of glutamine synthetase in starved (30-40%), streptozotocin-diabetic (17%), dexamethasone-treated (18-22%), laparotomized (25-27%) and septic rats (24-45%).
Ardawi
M S
MS
Department of Clinical Biochemistry, College of Medicine and Allied Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
D000138:Acidosis / Q000378:metabolism*; D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism*; D005973:Glutamine / Q000096:biosynthesis*; D007813:Laparotomy; D008168:Lung / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D009132:Muscles / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D018805:Sepsis / Q000378:metabolism*; D013217:Starvation / Q000378:metabolism*
D005973:Glutamine; D003907:Dexamethasone; D005974:Glutamate-Ammonia Ligase