Porcine pancreas extract decreases blood-ionized calcium in mice and inhibits osteoclast formation and bone resorption in culture.
Patients with acute pancreatitis commonly manifest hypocalcemia for reasons which are unknown. We found that porcine pancreas extracts (PX) significantly decreased blood-ionized calcium in Balb/c mice. Partially-purified PX with a molecular mass of approximately 27 kDa decreased blood-ionized calcium in the mice. Partially-purified PX suppressed not only 45Ca release from fetal rat long bones which had been stimulated by parathyroid hormone, interleukin-1 alpha, tumor necrosis factor, transforming growth factor-alpha, 1,25-dihydroxyvitamin D3 and prostaglandin E2, but tartrate-resistant acid phosphatase-positive multinucleated cell formation in the presence of 1,25-dihydroxyvitamin D3 in mouse marrow cultures. The results suggest that there is an as yet-unidentified bone metabolism-regulating substance in porcine pancreas which might be responsible for the hypocalcemia associated with acute pancreatitis.
Yoneda
T
T
Department of Medicine, University of Texas Health Science Center, San Antonio 78284-7877.
Takaoka
Y
Y
Alsina
M M
MM
Garcia
J
J
Mundy
G R
GR
D000818:Animals; D001835:Body Weight; D001862:Bone Resorption; D002118:Calcium / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C; D010010:Osteoclasts / Q000502:physiology*; D010179:Pancreas / Q000502:physiology*; D010180:Pancreatectomy; D010281:Parathyroid Hormone / Q000494:pharmacology; D013552:Swine; D014020:Tissue Extracts / Q000494:pharmacology
D010281:Parathyroid Hormone; D014020:Tissue Extracts; D002118:Calcium