Messenger RNA expression of the cytokine gene cluster, interleukin 3 (IL-3), IL-4, IL-5, and granulocyte/macrophage colony-stimulating factor, in allergen-induced late-phase cutaneous reactions in atopic subjects.
Cryostat sections from skin biopsies from 24-h allergen-induced late-phase cutaneous reactions (LPR) in 14 human atopic subjects were hybridized with 35S-labeled RNA probes for a number of cytokines. mRNA was detected for interleukin 3 (IL-3) (8/14), IL-4 (10/14), IL-5 (11/14), and granulocyte/macrophage colony-stimulating factor (GM-CSF) (13/14). Only 5 of 14 gave hybridization signals for IL-2, and 0 of 14 for interferon gamma. Biopsies from diluent controls gave only occasional weak signals. These results suggest that cells infiltrating the site of the 24-h LPR transcribe mRNA for the IL-3, IL-4, IL-5, and GM-CSF gene cluster and support the hypothesis that atopy is associated with preferential activation of cells having a similar cytokine profile to the murine T helper type 2 subset.
Kay
A B
AB
Department of Allergy and Clinical Immunology, National Heart & Lung Institute, London, UK.
Ying
S
S
Varney
V
V
Gaga
M
M
Durham
S R
SR
Moqbel
R
R
Wardlaw
A J
AJ
Hamid
Q
Q
D000293:Adolescent; D000328:Adult; D000485:Allergens; D001706:Biopsy; D016207:Cytokines / Q000235:genetics*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics*; D006801:Humans; D006968:Hypersensitivity, Delayed; D007377:Interleukin-3 / Q000235:genetics*; D015847:Interleukin-4 / Q000235:genetics*; D015848:Interleukin-5 / Q000235:genetics*; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics*; D012867:Skin / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology
D000485:Allergens; D016207:Cytokines; D007377:Interleukin-3; D015848:Interleukin-5; D012333:RNA, Messenger; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor