G-banding improvement for the MAC method.
Satisfactory identification of the neoplastic cell as well as an optimal G-banding of its chromosomes are routinely obtained in our laboratory using a modification of the method described by Knuutila et al. Mitotic cells are first identified by either cytochemical or immunological staining. For G-banding, the preparations are then destained with methanol:acetic acid, treated with 2 x SSC at 65 degrees C and restained with Wright stain.
Pérez
A
A
Unidad de Hematología y Oncología, Hospital de la Cruz Roja de Barcelona, Spain.
Solé
F
F
Caballín
R
R
Tarrida
N
N
Woessner
S
S
D002871:Chromosome Banding / Q000379:methods*; D003584:Cytological Techniques; D006801:Humans; D016130:Immunophenotyping / Q000379:methods