Characterization of Listeria monocytogenes isolates by Southern blot hybridization.
A synthetic deoxyribonucleotide probe for virulent Listeria monocytogenes, designated ADO7, was evaluated for its ability to identify restriction fragments of L. monocytogenes with nucleic acid sequences homologous with the beta-hemolysin gene by Southern blot hybridization of clinical and food isolates. The synthetic probe hybridized with three restriction fragments (approximately 1.1, 0.86, and 0.76 kb) of the serotype 1/2A isolates. Southern blot hybridization of the serogroup 4B isolates indicated that the nucleic acid sequences homologous with the beta-hemolysin gene probe were limited to a single restriction fragment of approximately 1 kb.
Wesley
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Physiopathology Research Unit, USDA-Agricultural Research Service, Ames, IA.
Wesley
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Heisick
J
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Harrell
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Wagner
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D
D000818:Animals; D015139:Blotting, Southern; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D004196:Disease Outbreaks; D005516:Food Microbiology; D006460:Hemolysin Proteins / Q000235:genetics; D006801:Humans; D008089:Listeria monocytogenes / Q000235:genetics*; D008088:Listeriosis / Q000453:epidemiology / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D004269:DNA, Bacterial; D006460:Hemolysin Proteins