Colony-stimulating factor 1 expression is down-regulated during the adipocyte differentiation of H-1/A marrow stromal cells and induced by cachectin/tumor necrosis factor.
We isolated clonal sublines of the established mouse marrow stromal cell line, H-1. These clonal sublines underwent differentiation into adipocytes in various degrees. One subline, H-1/A, underwent adipocyte differentiation after confluence, while another subline, H-1/D, did not differentiate. In H-1/A cells, the 4.5- and 2.5-kb major mRNA species of colony-stimulating factor 1 (CSF-1) were expressed before differentiation and were down-regulated at a posttranscriptional level during the differentiation of H-1/A cells. The down-regulation of the CSF-1 gene was not a result of arrested cellular growth, because no down-regulation was detected in the nondifferentiating sister line, H-1/D. This down-regulation appeared to be an early event in differentiation. Cachectin/tumor necrosis factor transiently induced the expression of CSF-1 and inhibited the differentiation of H-1/A cells into adipocytes. This induced expression of CSF-1 was due to an increased rate of transcription.
Umezawa
A
A
Department of Pathology, Keio University School of Medicine, Tokyo, Japan.
Tachibana
K
K
Harigaya
K
K
Kusakari
S
S
Kato
S
S
Watanabe
Y
Y
Takano
T
T
D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects; D000818:Animals; D001853:Bone Marrow; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells; D003114:Colony-Forming Units Assay; D003609:Dactinomycin / Q000494:pharmacology; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000166:cytology; D007700:Kinetics; D016173:Macrophage Colony-Stimulating Factor / Q000235:genetics*; D051379:Mice; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D003609:Dactinomycin; D016173:Macrophage Colony-Stimulating Factor; D005954:Glucosephosphate Dehydrogenase