Isolation of cDNA fragments hybridizing to rat brain-specific mRNAs.
A cDNA minilibrary in pUC18 has been generated from 3-month-old rat brains. Two hundred clones were randomly selected and sequenced. Comparison with a nucleic acid and protein data bank revealed a number of cDNA fragments not homologous to any published sequence. Northern blot analyses of some of these clones yielded 5 brain-specific cDNA fragments. The full-length cDNA for one of these clones has been isolated and completely sequenced. It corresponds to an mRNA of about 1,500 nucleotides, which is present in brain and, to a lesser extent, in heart and skeletal muscle. Its expression is developmentally regulated in the rat brain from 14-day embryos to 3-month-old adults. A very recent comparison with the EMBL nucleotide sequence data bank showed that this mRNA codes for a brain-specific snRNP-associated protein.
Esposito
F
F
Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli, Italia.
Ammendola
R
R
Duilio
A
A
Costanzo
F
F
Giordano
M
M
Zambrano
N
N
D'Agostino
P
P
Russo
T
T
Cimino
F
F
D000375:Aging; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D001921:Brain / Q000196:embryology / Q000254:growth & development / Q000502:physiology*; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015723:Gene Library; D005865:Gestational Age; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity; D010957:Plasmids; D018620:PrPSc Proteins; D011328:Prions / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics
D009419:Nerve Tissue Proteins; D018620:PrPSc Proteins; D011328:Prions; D012333:RNA, Messenger; D014764:Viral Proteins; D004247:DNA