Stability of mutant actins.
Mutants of the Drosophila Act88F actin gene were transcribed and translated in vitro and their relative stabilities were examined using urea gradient gel electrophoresis. Most of the mutant actins (E334K, E364K, G366D, G368E and R372H) were as stable as the wild-type. V339I had a slight decrease in stability, and E316K was the least stable. The causes of the differences are discussed and contrasted with the behaviour of the mutants in vivo, where E316K has normal stability and V339I is the least stable.
Drummond
D R
DR
Department of Biology, University of York, Heslington, U.K.
Hennessey
E S
ES
Sparrow
J C
JC
D000199:Actins / Q000737:chemistry* / Q000235:genetics; D000818:Animals; D004330:Drosophila; D004355:Drug Stability; D004591:Electrophoresis, Polyacrylamide Gel; D009154:Mutation; D014176:Protein Biosynthesis; D011489:Protein Denaturation; D011817:Rabbits; D014158:Transcription, Genetic; D014508:Urea
D000199:Actins; D014508:Urea