title: Isotype distribution of mucosal IgG-producing cells in patients with various IgG subclass deficiencies.
abstract: The subclass distribution of IgG-producing immunocytes was examined by immunohistochemistry in nasal and rectal mucosa of infection-prone patients with untreated IgG subclass deficiencies. Biopsy specimens from the two sites were obtained in 18 clinically and serologically well-characterized adult subjects; only a nasal or rectal sample was available from nine similar patients. Chronic lung disease was common in the patient groups with selective serum IgG1 deficiency and combined IgG1 and IgG3 deficiency, whereas the other categories of patients had mainly upper airway and other mild infections. Serum IgG2 or IgG3 deficiency was usually expressed also at the cellular level in rectal mucosa, and the proportion of rectal IgG1 cells was significantly correlated with the IgG1 level (r = 0.90, P less than 0.001). Likewise, there tended to be a decreased expression of the actual subclass at the cellular level in nasal mucosa of patients with serum IgG1 or IgG2 deficiency. Conversely, the median nasal proportion of IgG3 cells was remarkably unaffected by a deficiency of this subclass in serum and rectal mucosa. Interestingly, these patients rather tended to have raised IgG3 and reduced IgG2 cell proportions in their nasal mucosa, although this apparent local IgG3 compensation was nevertheless strongly correlated with the serum IgG3 level (r = 0.87, P less than 0.002). These disparities may reflect different antigenic and mitogenic exposure of the two tissue sites; for example, a persistent protein bombardment of the nasal mucosa that could conceivably override locally a B cell maturation defect. The possible clinical consequences of such variable mucosal expression of IgG subclass deficiencies remain to be studied.
authors.affiliation: Laboratory for Immunohistochemistry and Immunopathology (LIIPAT), University of Oslo, National Hospital, Norway.
authors.forename: D E
authors.initials: DE
authors.lastname: Nilssen
authors.forename: R
authors.initials: R
authors.lastname: Söderström
authors.forename: P
authors.initials: P
authors.lastname: Brandtzaeg
authors.forename: K
authors.initials: K
authors.lastname: Kett
authors.forename: L
authors.initials: L
authors.lastname: Helgeland
authors.forename: G
authors.initials: G
authors.lastname: Karlsson
authors.forename: T
authors.initials: T
authors.lastname: Söderström
authors.forename: L A
authors.initials: LA
authors.lastname: Hanson
mesh_terms: D000328:Adult; D000368:Aged; D000921:Antibody-Producing Cells / Q000378:metabolism*; D004406:Dysgammaglobulinemia / Q000150:complications / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D017099:IgG Deficiency; D007109:Immunity; D007132:Immunoglobulin Isotypes / Q000378:metabolism*; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology*; D008171:Lung Diseases / Q000150:complications; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000166:cytology / Q000276:immunology*; D012007:Rectum / Q000166:cytology / Q000276:immunology
chemical_list: D007132:Immunoglobulin Isotypes