Human thyroid hormone beta 1 receptor produced by recombinant baculovirus-infected insect cells.
Thyroid hormone receptors are nuclear proteins which regulate transcription in a hormone dependent manner. The baculovirus expression system was used for the overexpression of the beta 1 isoform of the human thyroid hormone receptor. The baculovirus produced receptor binds tri-iodothyronine with high affinity, is specifically immunoprecipitated with a beta 1 specific antibody, and binds to DNA that contains a known thyroid hormone receptor recognition site. Large scale production and purification of baculovirus produced receptor will be useful for structure-function analyses and studies of transcriptional regulation.
zu Putlitz
J
J
Thyroid Unit, Massachusetts General Hospital, Boston.
Datta
S
S
Madison
L D
LD
Macchia
E
E
Jameson
J L
JL
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005822:Genetic Vectors; D006801:Humans; D007313:Insecta; D007700:Kinetics; D008970:Molecular Weight; D011988:Receptors, Thyroid Hormone / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014158:Transcription, Genetic; D014284:Triiodothyronine / Q000378:metabolism
D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine