Isolation of a new gene (SW A2) encoding an alpha-amylase from Schwanniomyces occidentalis and its expression in Saccharomyces cerevisiae.
A new gene (SW A2) encoding a secretory alpha-amylase activity from Schwanniomyces occidentalis has been cloned from this yeast and then expressed in Saccharomyces cerevisiae. Both Sw, occidentalis and a transformant of S. cerevisiae incorporating SW A2 contain a transcript of 2.1 kb which hybridizes to DNa carrying the SW A2 gene. This indicates that the transcript is a product of the SW A2 gene. Transcription of the SW A2 gene seems to be regulated in both Sw. occidentalis and S. cerevisiae. Furthermore, the secretion of alpha-amylase activity is drastically repressed by glucose in both Sw. occidentalis and a transformant of S. cerevisiae containing SW A2.
Abarca
D
D
Centro de Biologia Molecular (CSIC-UAM), Universidad Autónoma, Spain.
Fernández-Lobato
M
M
del Pozo
L
L
Jiménez
A
A
D004591:Electrophoresis, Polyacrylamide Gel; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D010957:Plasmids; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D004718:Saccharomycetales / Q000201:enzymology*; D014158:Transcription, Genetic; D000516:alpha-Amylases / Q000235:genetics*
D000516:alpha-Amylases