Ha-ras-1 alleles in Norwegian lung cancer patients.
We have examined DNA restriction fragment length polymorphisms (RFLP) of the Ha-ras-1 gene in DNA from 118 lung cancer patients and 123 unaffected controls. When DNA samples were digested with MspI/HpaII restriction endonucleases. Southern blot analysis demonstrated 4 common, 4 intermediate and 7 different rare alleles in the combined population after hybridization to the pGDa1 probe. Six of the rare alleles were unique for the lung cancer group and 1 rare allele for the control group. The frequency of rare alleles in lung cancer patients (10/236) was significantly different (P less than 0.01) from the control group (1/246). The lung cancer group also had a significantly lower frequency of the common 2.57 kb fragment than the controls (P less than 0.02). The results thus indicate that Ha-ras genotyping may be of value in lung cancer risk assessment.
Ryberg
D
D
Department of Toxicology, National Institute of Occupational Health, Oslo, Norway.
Tefre
T
T
Ovrebø
S
S
Skaug
V
V
Stangeland
L
L
Naalsund
A
A
Baera
R
R
Børresen
A L
AL
Haugen
A
A
D000483:Alleles; D015139:Blotting, Southern; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics; D011905:Genes, ras; D005838:Genotype; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
D015342:DNA Probes; D004273:DNA, Neoplasm