Effect of d-mannose on fimbriae of bacterial isolates from chicken carcasses.
Pseudomonad-like microbial isolates were obtained from commercially processed broiler chicken carcasses for use in experiments to determine if d-mannose would interfere with their ability to form pellicles and rims and prevent agglutination of blood and yeast cells. Inhibition of agglutination, rim formation, and pellicle formation would provide evidence that d-mannose acts on the fimbriae to prevent attachment of microorganisms to surfaces. Presence of 4% d-mannose in the growth medium interfered with the formation and stability of the pellicle in some isolates. The pellicles affected by d-mannose did not cover the entire surface and were easily dispersed. Addition of d-mannose did not prevent the formation of rims to the same extent it prevented pellicle formation, and it was concluded that d-mannose did not completely prevent growth of fimbriae. The addition of d-mannose prevented the agglutination of 1% sheep blood, 1% horse blood, and yeast cells by interfering with the formation of fimbriae or the mechanism of attachment by fimbriae. These experiments provided evidence that d-mannose can be used to prevent attachment of isolates normally found on chicken carcasses to specific cell surfaces by occupying mannose-sensitive receptor sites on the cell.
deGraft-Hanson
J
J
Department of Poultry Science, University of Maryland, College Park 20742.
Heath
J L
JL
D000372:Agglutination Tests; D000818:Animals; D001419:Bacteria / Q000187:drug effects* / Q000648:ultrastructure; D001422:Bacterial Adhesion; D002645:Chickens / Q000382:microbiology*; D004926:Escherichia coli / Q000187:drug effects / Q000648:ultrastructure; D010861:Fimbriae, Bacterial / Q000187:drug effects*; D005516:Food Microbiology; D006384:Hemagglutination; D008358:Mannose / Q000494:pharmacology*; D011548:Pseudomonadaceae / Q000187:drug effects / Q000648:ultrastructure; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000648:ultrastructure
D008358:Mannose