A rare-event analysis model for quantifying white cells in white cell-depleted blood.
An analysis model to detect and quantify white cells (WBCs) in red cell concentrates (RBCC) drawn from units of blood that are highly depleted of WBCs is described. WBC detection is performed by fluorescence analysis of 50 microL of RBCC labeled with propidium iodide, a DNA/RNA fluorophore. Quantification is performed by regression analysis of standard dilutions of RBCC in substantially WBC-free red cells. This RBCC diluent is obtained by filtration of blood through a new medium. The method proves to be precise (CV = 7%), efficient (+/- 30 min/aliquot), and linear (r = 0.99) to 6 log10 WBC depletion of the native product. The current technique is preferable to those suggested previously, such as ficoll concentration, which requires the sacrifice of the unit of blood for counting purposes, and to earlier fluorescence analysis techniques that do not employ WBC-free red cell diluents. The latter do not monitor extremely low concentrations of WBCs because they lack adequate signal-to-noise discrimination. The sensitivity of the described method allows for monitoring of WBC depletion procedures with greater efficiency than is currently available commercially.
Wenz
B
B
Department of Laboratory Medicine, Albert Einstein College of Medicine, New York, New York.
Burns
E R
ER
Lee
V
V
Miller
W K
WK
D001781:Blood Component Removal; D002469:Cell Separation; D017707:Erythrocyte Transfusion; D005456:Fluorescent Dyes; D006801:Humans; D007958:Leukocyte Count / Q000379:methods*; D007962:Leukocytes / Q000166:cytology; D011419:Propidium; D012044:Regression Analysis
D005456:Fluorescent Dyes; D011419:Propidium