title: The structure of the dihaem cytochrome b of fumarate reductase in Wolinella succinogenes: circular dichroism and sequence analysis studies.
abstract: The fumarate reductase from Wolinella succinogenes contains two haem groups with markedly different midpoint potentials (-20 mV and -200 mV). The enzyme is made up of three subunits, the lipophilic one of which (cytochrome b) ligates the haems. Circular dichroism (CD) spectroscopy has been applied to the reductase in order to obtain information on the structure of the haems and of their environment. This approach is integrated with amino acid sequence comparison of the cytochrome b with other quinone-reacting membrane haemoproteins for predicting the axial ligands of the haems as well as their location relative to the membrane. The following results have been obtained: (1) the CD spectra in the Soret region show exciton coupling indicating haem-haem interaction, which is particularly evident in the reduced state and disappears upon denaturation of the enzyme; (2) The apoprotein of cytochrome b is predicted to consist of five hydrophobic helices (helices A-D and cd), four of which should span the membrane. Helices A, B, C and cd contain a histidine residue each which possibly forms one of the ligands of the haems. It is proposed that haem b (-20 mV) is ligated by H44 and H93, and haem b (-200 mV) by H143 and H182.
authors.affiliation: Dept. of Biology, University of Bologna, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Degli Esposti
authors.forename: M
authors.initials: M
authors.lastname: Crimi
authors.forename: C
authors.initials: C
authors.lastname: Körtner
authors.forename: A
authors.initials: A
authors.lastname: Kröger
authors.forename: T
authors.initials: T
authors.lastname: Link
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology*; D002942:Circular Dichroism; D003573:Cytochrome b Group / Q000737:chemistry / Q000648:ultrastructure*; D006639:Histidine / Q000502:physiology; D008565:Membrane Proteins / Q000737:chemistry / Q000648:ultrastructure; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012995:Solubility; D013385:Succinate Dehydrogenase / Q000737:chemistry / Q000648:ultrastructure*
chemical_list: D003573:Cytochrome b Group; D008565:Membrane Proteins; D006639:Histidine; D013385:Succinate Dehydrogenase