title: Cell culture amplification of a defective Marek's disease virus.
abstract: A highly amplified 4-kb EcoRI fragment was present in DNA isolated from high cell culture passaged stocks (greater than 93 passages) of 281MI/1, a serotype 2 Marek's disease virus (MDV). The isolated 4-kb fragment is amplified in the presence of MDV, replicating as a high molecular weight, head-to-tail concatemer. When the 4-kb fragment was cloned into pUC18 and cotransfected with MDV DNA into chicken embryo fibroblast cells, the plasmid clone also replicated as a high molecular weight concatemer.
authors.affiliation: U.S. Department of Agriculture, Regional Poultry Research Laboratory, East Lansing, MI 48823.
authors.forename: J K
authors.initials: JK
authors.lastname: Carter
authors.forename: R F
authors.initials: RF
authors.lastname: Silva
mesh_terms: D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D015342:DNA Probes; D005784:Gene Amplification; D005814:Genes, Viral; D008381:Herpesvirus 2, Gallid / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D014162:Transfection
chemical_list: D015342:DNA Probes; D004247:DNA