Postnatal development and isolation of peroxisomes from brain.
We analyzed the postnatal peroxisome development in rat brain by measuring the enzyme activities of catalase and acyl-CoA oxidase and beta-oxidation of [1-14C]lignoceric acid. These enzyme activities were higher between 10 and 16 days of postnatal life and then decreased. We developed and compared two different methods for isolation of enriched peroxisomes from 10-day-old rat brain by using a combination of differential and density gradient centrifugation techniques. Peroxisomes in Percoll (self-generating gradient) banded at a density of 1.036 +/- 0.012 g/ml and in Nycodenz continuous gradient at 1.125 +/- 0.014 g/ml. Acyl-CoA oxidase, D-amino acid oxidase, L-pipecolic acid oxidase, and dihydroxyacetone phosphate acyltransferase activities and activities for the oxidation of very long chain fatty acid (lignoceric acid) were almost exclusively associated with catalase activity (a marker enzyme for peroxisomes) in the gradient. The postnatal increase in peroxisomal activity with the onset of myelination and the presence of enzyme for the biosynthesis of plasmalogens and oxidation of very long chain fatty acid (both predominant constituents of myelin) suggest that brain peroxisomes may play an important role in the assembly and turnover of myelin.
Lazo
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Department of Pediatrics, Medical University of South Carolina, Charleston 29425.
Singh
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Singh
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D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D001921:Brain / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure*; D002499:Centrifugation, Density Gradient; D005227:Fatty Acids / Q000378:metabolism; D008830:Microbodies / Q000378:metabolism / Q000502:physiology*; D008928:Mitochondria / Q000378:metabolism; D010084:Oxidation-Reduction; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000648:ultrastructure
D005227:Fatty Acids; D010169:Palmitic Acids; D019308:Palmitic Acid; C010210:lignoceric acid