Inhibition of human immunodeficiency virus type 1 replication in human T cells stably expressing antisense RNA.
Human T-lymphoid cell lines with constitutive intracellular expression of antisense RNA complementary to a 407-bp sequence of the 5' leader-gag region of human immunodeficiency virus type 1 were established by using a nonretroviral expression vector. In cell lines with antisense RNA expression detectable by Northern (RNA) hybridization, human immunodeficiency virus type 1 replication was inhibited to 88% 10 days postinfection and this inhibition lasted 3 weeks postinfection.
Sczakiel
G
G
Institut für Virusforschung, Deutsches Krebsforschungszentrum, Heidelberg, Federal Republic of Germany.
Pawlita
M
M
D002460:Cell Line; D002472:Cell Transformation, Viral; D002877:Chromosomes, Human; D002999:Clone Cells; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D016372:RNA, Antisense / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D013601:T-Lymphocytes; D013997:Time Factors; D014162:Transfection; D014779:Virus Replication
D016372:RNA, Antisense; D012367:RNA, Viral