Search for cellular phosphorylation products of D-allose.
The all-cis hexose D-allose, fed to hamster fibroblast cultures over 14-20 hr, brings about a striking down-regulation of hexose transport. This down-regulation by allose is inhibited by 2,4-dinitrophenol. By using D-[14C]allose and thin-layer chromatography, two types of products accumulated that have been identified as allose and allose phosphate. We suspect that allose phosphate might be the down-regulator.
Ullrey
D B
DB
Chemistry Department, Boston University, MA 02215.
Kalckar
H M
HM
D000818:Animals; D001711:Biotransformation; D002250:Carbon Radioisotopes; D002460:Cell Line; D002855:Chromatography, Thin Layer; D006224:Cricetinae; D003412:Cricetulus; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D008168:Lung; D010766:Phosphorylation; D011865:Radioisotope Dilution Technique
D002250:Carbon Radioisotopes; C002055:allose; D005947:Glucose