Isolation and characterization of 15K noncollagenous protein from bovine periodontal ligament.
In pursuit of the nature of noncollagenous proteins (NCP) and their biosynthesis in periodontal ligament (PDL), isolation, characterization and a short incubation study were made on the PDL of the bovine mandibular incisors. A sodium chloride extract from the PDL contained several noncollagenous protein components. Eliminating serum derived proteins by a cross-reaction with antibody against bovine serum proteins, a protein estimated to be 15K on 5-15% gradient NaDodSO4-gel was the major constituent in the extract. This protein was isolated by sequential chromatography using Sepharose CL-6B and Sephacryl S-200 under dissociative conditions (4M guanidium hydrochloride). A chemical analysis of the protein showed that it was rich in aspartic acid, glutamic acid, glycine, alanine, leucine and lysine, and contained 5% sugars. Antibody directed against the purified PDL 15K protein reacted with the 15K protein, but no cross-reaction was observed with other components from the bovine PDL. A biosynthetic study of the protein using a short incubation with 3H-leucine indicated that the PDL cells produced the 15K protein and secreted it into the medium.
Sasaki
Y
Y
Kanagawa Dental College, Japan.
D000409:Alanine / Q000032:analysis; D000818:Animals; D001224:Aspartic Acid / Q000032:analysis; D002417:Cattle; D002850:Chromatography, Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005971:Glutamates / Q000032:analysis; D018698:Glutamic Acid; D007930:Leucine / Q000032:analysis; D008239:Lysine / Q000032:analysis; D010513:Periodontal Ligament / Q000737:chemistry* / Q000378:metabolism; D014176:Protein Biosynthesis; D011506:Proteins / Q000302:isolation & purification; D011863:Radioimmunoassay
D005971:Glutamates; D011506:Proteins; D001224:Aspartic Acid; D018698:Glutamic Acid; D007930:Leucine; D008239:Lysine; D000409:Alanine