title: A clonal CD4-positive T-cell line established from the blood of a patient with Sézary syndrome.
abstract: The reported inability to establish long-term T-cell lines from the blood of cutaneous T-cell lymphoma patients with circulating neoplastic T cells has hindered the development of an in vitro system to investigate Sézary syndrome. We have established a rapidly proliferating T-cell line from the peripheral blood of a patient with Sézary syndrome, which expresses a mature helper T-cell phenotype and contains cytogenetic abnormalities and T-cell receptor gene rearrangements identical to those in the patient's blood. The method of establishment and characteristics of this line are described.
authors.affiliation: Wistar Institute, Philadelphia, PA 19104.
authors.forename: J T
authors.initials: JT
authors.lastname: Abrams
authors.forename: S
authors.initials: S
authors.lastname: Lessin
authors.forename: S K
authors.initials: SK
authors.lastname: Ghosh
authors.forename: W
authors.initials: W
authors.lastname: Ju
authors.forename: E C
authors.initials: EC
authors.lastname: Vonderheid
authors.forename: P
authors.initials: P
authors.lastname: Nowell
authors.forename: G
authors.initials: G
authors.lastname: Murphy
authors.forename: B
authors.initials: B
authors.lastname: Elfenbein
authors.forename: E
authors.initials: E
authors.lastname: DeFreitas
mesh_terms: D000368:Aged; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis*; D002460:Cell Line; D046508:Culture Techniques / Q000379:methods; D015342:DNA Probes; D004261:DNA Replication / Q000187:drug effects; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation; D010641:Phenotype; D012751:Sezary Syndrome / Q000276:immunology*; D012878:Skin Neoplasms / Q000276:immunology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000648:ultrastructure
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface; D015704:CD4 Antigens; D015342:DNA Probes; D004273:DNA, Neoplasm; D007376:Interleukin-2