title: The isolation of cDNA clones for CD48.
abstract: HuLy-m3 is an Mr 47,000 pan-leukocyte antigen detected by the monoclonal antibody (mAb) 5-4.8. This report describes the isolation and analysis of a cDNA clone encoding HuLy-m3. Serological analysis demonstrated that antibodies of the CD48 cluster also reacted with transfected cells expressing HuLy-m3. The DNA sequence of the clone suggests linkage to the cell membrane through a glycosyl phosphatidylinositol tail and this was verified experimentally. Sequence similarity with the human B-cell activation antigen Blast-1 was noted.
authors.affiliation: Department of Pathology, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: H A
authors.initials: HA
authors.lastname: Vaughan
authors.forename: M M
authors.initials: MM
authors.lastname: Henning
authors.forename: D F
authors.initials: DF
authors.lastname: Purcell
authors.forename: I F
authors.initials: IF
authors.lastname: McKenzie
authors.forename: M S
authors.initials: MS
authors.lastname: Sandrin
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics*; D000943:Antigens, Differentiation / Q000096:biosynthesis / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern; D000071178:CD48 Antigen; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D006801:Humans; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011233:Precipitin Tests; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; D000954:Antigens, Surface; D000071178:CD48 Antigen; C000604065:CD48 protein, human; D008562:Membrane Glycoproteins