title: A Ki-1 (CD30)-positive T (E+, CD4+, Ia+)-cell line, DL-40, established from aggressive large cell lymphoma.
abstract: A new human lymphoma cell line, designated DL-40, was established from the peripheral blood of a 64-year-old woman with leukemic conversion of aggressive large cell lymphoma. The cell line grew in suspension with or without forming clumps of cells and exhibited large, round, or multiple nuclei in the relatively abundant cytoplasm that was positive for acid phosphatase. The cells expressed a Ki-1 antigen (CD30), E+, CD2+, CD4+, CD45+, Ia+ phenotype and had rearranged T-cell receptor beta chain but were negative for CD15, HTLV-I, and Epstein-Barr virus nuclear antigen. Chromosome analysis of this cell line showed a human female karyotype with complex hyperdiploid abnormalities. DL-40 cells produced tumors histologically similar to the original lymphoma when transplanted into nude mice and immunosuppressed hamsters. The DL-40 cell line could provide a useful tool for the understanding of biology of the Ki-1-positive non-Hodgkin's lymphoma.
authors.affiliation: Department of Medicine, Kochi Medical School, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Kubonishi
authors.forename: H
authors.initials: H
authors.lastname: Sonobe
authors.forename: T
authors.initials: T
authors.lastname: Miyagi
authors.forename: Y
authors.initials: Y
authors.lastname: Iwahara
authors.forename: J H
authors.initials: JH
authors.lastname: Ohyashiki
authors.forename: K
authors.initials: K
authors.lastname: Ohyashiki
authors.forename: K
authors.initials: K
authors.lastname: Toyama
authors.forename: Y
authors.initials: Y
authors.lastname: Ohtsuki
authors.forename: I
authors.initials: I
authors.lastname: Miyoshi
mesh_terms: D000943:Antigens, Differentiation / Q000096:biosynthesis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis; D000951:Antigens, Neoplasm / Q000096:biosynthesis; D014408:Biomarkers, Tumor; D018801:CD2 Antigens; D015704:CD4 Antigens / Q000096:biosynthesis; D005260:Female; D005819:Genetic Markers; D006649:Histocompatibility Antigens / Q000096:biosynthesis; D006801:Humans; D066298:In Vitro Techniques; D007621:Karyotyping; D017730:Ki-1 Antigen; D017493:Leukocyte Common Antigens; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics / Q000276:immunology*; D008875:Middle Aged; D011971:Receptors, Immunologic / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
chemical_list: D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D018801:CD2 Antigens; D015704:CD4 Antigens; D005819:Genetic Markers; D006649:Histocompatibility Antigens; D017730:Ki-1 Antigen; D011971:Receptors, Immunologic; D017493:Leukocyte Common Antigens