Rejection of class I MHC-deficient haemopoietic cells by irradiated MHC-matched mice.
Irradiated MHC-heterozygous mice often reject bone marrow cells transplanted from one of the homozygous parental strains, a phenomenon ('hybrid resistance') that appears to violate the laws of transplantation. Rejection of parental and allogeneic marrow cells also differs from conventional T cell-mediated rejection mechanisms as it is effected by NK1.1+ cells. To account for the unusual specificity of bone marrow rejection, it has been proposed that NK1.1+ cells destroy marrow cells that fail to express the full complement of self MHC class I (MHC-I) molecules. We show here that NK1.1+ cells in normal mice reject haemopoietic transplants from mice that are deficient for normal cell-surface MHC-I expression because of a targeted mutation in the beta 2-microglobulin gene. These findings demonstrate that deficient expression of MHC-I molecules renders marrow cells susceptible to rejection.
Bix
M
M
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
Liao
N S
NS
Zijlstra
M
M
Loring
J
J
Jaenisch
R
R
Raulet
D
D
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000954:Antigens, Surface / Q000276:immunology; D001853:Bone Marrow / Q000528:radiation effects; D016026:Bone Marrow Transplantation / Q000276:immunology*; D006084:Graft Rejection; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000172:deficiency* / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D008817:Mice, Mutant Strains; D008954:Models, Biological; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014916:Whole-Body Irradiation
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D015395:Histocompatibility Antigens Class I; D011948:Receptors, Antigen, T-Cell