IL-1 induces ornithine decarboxylase in normal T lymphocytes.
IL-1 alpha regulation of ornithine decarboxylase (ODC) was examined in T cells because IL-1 is a costimulus for T cell proliferation and ODC is a critical enzyme in the metabolic events associated with cellular proliferation. In the present study, we demonstrate that IL-1 alpha induces ODC mRNA and ODC enzyme activity in T cells. Unlike many IL-1 actions on T cells, this did not require a costimulus from the TCR, IL-1 alone being sufficient to induce ODC. The mechanism of IL-1 induction of ODC probably operates at several levels, including transcription, mRNA stability, and translation. Previous studies have shown that IL-1 prepares T cells for replication by increasing the production of c-jun, c-fos, c-myc, growth factors, growth factor receptors, and the response to growth factors. From the present study, ODC induction can be added to the list of IL-1-induced replicative machinery in T cells.
Bristol
L A
LA
Laboratory of Molecular Immunoregulation, Program Resources, Inc., Frederick, MD.
Smith
M R
MR
Bhat
N K
NK
Durum
S K
SK
D000818:Animals; D004790:Enzyme Induction; D005260:Female; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000502:physiology*; D007700:Kinetics; D008213:Lymphocyte Activation / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D009955:Ornithine Decarboxylase / Q000096:biosynthesis* / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis; D013154:Spleen / Q000201:enzymology; D013601:T-Lymphocytes / Q000201:enzymology*; D014158:Transcription, Genetic / Q000276:immunology
D007375:Interleukin-1; D012333:RNA, Messenger; D009955:Ornithine Decarboxylase