Photometric or fluorometric assay of cathepsin B, L and H and papain using substrates with an aminotrifluoromethylcoumarin leaving group.
N-trifluoromethylcoumarinylamide derivatives of benzyloxycarbonyl-Arg-Arg, benzyloxycarbonyl-Phe-Arg and Arg are convenient chromogenic and fluorogenic substrates of cathepsin B, L and H, respectively. Benzyloxycarbonyl-Phe-Arg-N-trifluoromethylcoumarinylamide is also a highly sensitive substrate for papain.
Tchoupe
J R
JR
INSERM Unité 237, Université Louis Pasteur de Strasbourg, Illkirch, France.
Moreau
T
T
Gauthier
F
F
Bieth
J G
JG
D000818:Animals; D002401:Cathepsin B / Q000378:metabolism*; D056655:Cathepsin H; D056668:Cathepsin L; D002403:Cathepsins / Q000378:metabolism*; D003374:Coumarins / Q000378:metabolism; D003546:Cysteine Endopeptidases; D010450:Endopeptidases; D008099:Liver / Q000201:enzymology; D010206:Papain / Q000378:metabolism*; D051381:Rats; D013050:Spectrometry, Fluorescence; D013379:Substrate Specificity
D003374:Coumarins; C055467:7-amino-4-trifluoromethylcoumarin; D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D002401:Cathepsin B; D056668:Cathepsin L; C534280:Ctsl protein, rat; D056655:Cathepsin H; C534128:Ctsh protein, rat; D010206:Papain