Gly429 is the major determinant of uncompetitive inhibition of human germ cell alkaline phosphatase by L-leucine.
The catalytic activity of human placental alkaline phosphatase (PLAP) and germ cell alkaline phosphatase (GCAP) can be inhibited, through an uncompetitive mechanism, by L-Phe. GCAP is also selectively inhibited by L-Leu. Site-directed mutagenesis of five of the 12 residues which are different in PLAP and GCAP revealed that Gly429 is the primary determinant of GCAP inhibition by L-Leu, and Ser84 and Leu297 play a modulatory role in the inhibition.
Hummer
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La Jolla Cancer Research Foundation, CA 92037.
Millán
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D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors* / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine / Q000494:pharmacology; D001483:Base Sequence; D005260:Female; D005998:Glycine; D006801:Humans; D007930:Leucine / Q000494:pharmacology*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D015345:Oligonucleotide Probes; D009842:Oligopeptides / Q000494:pharmacology; D010649:Phenylalanine / Q000494:pharmacology; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D015183:Restriction Mapping; D014162:Transfection
D015345:Oligonucleotide Probes; D009842:Oligopeptides; D010649:Phenylalanine; D001120:Arginine; D000469:Alkaline Phosphatase; D007930:Leucine; D005998:Glycine