title: Gastric cytoprotection by intracisternal interleukin-1 beta in the rat.
abstract: The effects of intracisternal (ic) injection of recombinant interleukin-1 beta (IL-1) on absolute ethanol-induced gastric necrotic lesions were studied in conscious rats. IL-1 given ic inhibited ethanol-induced gastric lesions. The cytoprotective effect was dose dependent (ED175 ng/rat), long lasting with a maximal action when given 1-3 h prior to ethanol, blocked by ic injection of a IL-1 receptor antagonist protein (IRAP), and by intraperitoneal injection of indomethacin. IL-1, injected ic, was detected in the peripheral blood. However, IL-1 serum levels were lower after IL-1 injection ic than after ip at a dose giving equal gastric protection. These data show that ic IL-1 induces long lasting gastric protection mediated by interaction with IL-1 receptors and prostaglandin pathways at central and/or peripheral sites that remain to be localized.
authors.affiliation: Safety Pharmacology, Upjohn Company, Kalamazoo, MI.
authors.forename: A
authors.initials: A
authors.lastname: Robert
authors.forename: E
authors.initials: E
authors.lastname: Saperas
authors.forename: W R
authors.initials: WR
authors.lastname: Zhang
authors.forename: A S
authors.initials: AS
authors.lastname: Olafsson
authors.forename: C
authors.initials: C
authors.lastname: Lancaster
authors.forename: D E
authors.initials: DE
authors.lastname: Tracey
authors.forename: J G
authors.initials: JG
authors.lastname: Chosay
authors.forename: Y
authors.initials: Y
authors.lastname: Taché
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D000431:Ethanol / Q000633:toxicity; D005260:Female; D007213:Indomethacin / Q000494:pharmacology; D007267:Injections; D007278:Injections, Spinal; D007375:Interleukin-1 / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D008297:Male; D009336:Necrosis; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D013116:Spinal Cord / Q000187:drug effects / Q000502:physiology*; D013270:Stomach / Q000187:drug effects / Q000473:pathology*
chemical_list: D007375:Interleukin-1; D011994:Recombinant Proteins; D000431:Ethanol; D007213:Indomethacin