[The role of intracellular processes in regulating neuronal sensitivity to acetylcholine].
Intracellular regulatory mechanisms of neuronal response to acetylcholine were studied on intracellularly perfused isolated neurones of Lymnaea stagnalis using voltage-clamp technique. It was found that at the change of concentration of free intracellular Ca2+ from 0.06 to 0.7 microM the inhibitory effect of intracellularly added serotonin depends on Ca2+, and the modulation of acetylcholine responses by intracellular serotonin is unchanged. The blockers of calmoduline trifluoperazine and W-7 inhibit inward acetylcholine current at both intra- and extracellular introduction. Possible mechanisms mediating the effect of intracellularly added serotonin on the membrane cholinoreceptors of neurones are discussed.
Iurchenko
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OP
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000502:physiology; D002147:Calmodulin / Q000037:antagonists & inhibitors; D066298:In Vitro Techniques; D008195:Lymnaea; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D018377:Neurotransmitter Agents / Q000502:physiology; D012701:Serotonin / Q000494:pharmacology; D013449:Sulfonamides / Q000494:pharmacology; D014268:Trifluoperazine / Q000494:pharmacology
D002147:Calmodulin; D018377:Neurotransmitter Agents; D013449:Sulfonamides; D014268:Trifluoperazine; D012701:Serotonin; C017967:W 7; D000109:Acetylcholine; D002118:Calcium