title: 1H and 119Sn magnetic resonance study of the SnIV protoporphyrin IX complex of equine myoglobin. Structure of the porphyrin-binding pocket.
abstract: Tin protoporphyrin IX (SnPP) is being used in the treatment of hyperbilirubinemia. We have studied the SnPP complex with equine myoglobin (EqMb) by 1H and 119Sn nuclear magnetic resonance spectroscopy (NMR) as a general model for SnPP interaction with hemoproteins. The complex formed from SnPP and EqMb, SnPP.EqMb, was found to have essentially the same porphyrin-binding pocket as EqMbCO, including the same porphyrin orientation in the major form of EqMbCO. 119Sn NMR spectroscopy has been used to demonstrate that the proximal His93F8-metal coordination is likely to be intact in SnPP.EqMb. Minor shifts in the side chain positions of some of the residues are indicated, possibly reflecting the presence of water in the sixth coordination site. SnPP.EqMb appears to be stable; it persists at room temperature for weeks and exhibits very slow exchange rates (2H for 1H) for a large number of amide protons in the pH range 7-9.
authors.affiliation: Chemistry Department, Portland State University, Oregon 97207-0751.
authors.forename: R S
authors.initials: RS
authors.lastname: Deeb
authors.forename: D H
authors.initials: DH
authors.lastname: Peyton
mesh_terms: D000818:Animals; D006736:Horses; D009682:Magnetic Resonance Spectroscopy; D008665:Metalloporphyrins / Q000737:chemistry*; D008958:Models, Molecular; D009211:Myoglobin / Q000737:chemistry*; D011524:Protoporphyrins / Q000737:chemistry*
chemical_list: D008665:Metalloporphyrins; D009211:Myoglobin; D011524:Protoporphyrins; C032628:tin protoporphyrin IX