Transformation of freshwater and marine caulobacters by electroporation.
We performed plasmid electrotransformation of Caulobacter crescentus strains and obtained up to 3 x 10(8) transformants per micrograms of pKT230. The presence and integrity of the paracrystalline protein surface (S) layer influenced electroporation; caulobacters lacking the S layer were electrotransformed 10 times more efficiently than caulobacters possessing the S layers. A procedure yielding 1,500 transformants per micrograms of pKT230 was developed for a marine caulobacter. Electroporation was used in combination with several genetic techniques, including introduction of ligation mixtures, suicide transposon mutagenesis, gene replacement, and plasmid electrotransfer from Escherichia coli to caulobacters.
Gilchrist
A
A
Department of Microbiology, University of British Columbia, Vancouver, Canada.
Smit
J
J
D001419:Bacteria / Q000235:genetics*; D018407:Bacterial Physiological Phenomena; D004269:DNA, Bacterial / Q000235:genetics; D004558:Electric Stimulation; D005618:Fresh Water; D007700:Kinetics; D010957:Plasmids; D012623:Seawater; D014169:Transformation, Bacterial
D004269:DNA, Bacterial