A nuclear factor requires Zn2+ to bind a regulatory MRE element of the mouse gene encoding metallothionein-1.
The metal ion requirement of nuclear proteins for binding to the metal regulatory element d(MREd) of the mouse gene encoding metallothionein-1 was investigated using an in vitro exonuclease III footprinting assay. The specific DNA-binding activity of the factor was inactivated by the chelating agents, EDTA and 1,10-phenanthroline. Binding activity was restored by Zn2+, but not by Cd2+. These results show that Zn2+ ions are a required component for specific in vitro DNA binding of the MREd-binding protein.
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Centre de Recherche en Cancérologie de l'Université Laval, L'Hôtel-Dieu de Québec, Canada.
D000818:Animals; D001483:Base Sequence; D002104:Cadmium / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005796:Genes; D008668:Metallothionein / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D012045:Regulatory Sequences, Nucleic Acid; D015032:Zinc / Q000494:pharmacology*
D004268:DNA-Binding Proteins; D002104:Cadmium; D008668:Metallothionein; D015032:Zinc