Class I and class II MHC gene products differentially affect the fate of V beta 5 bearing thymocytes.
We have previously shown that T cells bearing V beta 5+ T-cell receptors (TCRs) are frequent in B10 (H-2b) and B10.Q (H-2q) mouse strains but are rare in the congenic strain B10.BR (H-2k). Furthermore, we have found that V beta 5 bearing T cells appear to be excluded from the B10 alloresponse to I-Abm12 despite the participation of most other V beta bearing cells. To further study MHC effects on V beta 5 expression, we have generated two V beta 5 specific monoclonal antibodies and show here that V beta 5 expressing T cells are clonally deleted from strains expressing a class II, I-E molecule. Furthermore, I-E- strains generate few CD4+ V beta 5+ T cells despite significant numbers of V beta 5+ T cells in the CD8+ subset. Thus, V beta 5 bearing T cells are positively selected by class I MHC molecules, clonally deleted by class II I-E molecules, and poorly selected by class II I-A molecules.
Bill
J
J
Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206.
Kanagawa
O
O
Linten
J
J
Utsunomiya
Y
Y
Palmer
E
E
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D004247:DNA; D005434:Flow Cytometry; D006183:H-2 Antigens / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000502:physiology*; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D006822:Hybrid Cells; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology*; D016693:Receptors, Antigen, T-Cell, alpha-beta; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006183:H-2 Antigens; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; C024138:I-E-antigen; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta; D004247:DNA