Characterization of beta adrenoceptors on cultured endothelial cells by radioligand binding.
The properties of beta-adrenoceptors present on the cultured bovine pulmonary artery endothelial cells were studied by radioligand binding. These cell contain a high density of beta-adrenoceptors (approximately 16,000 receptors/cell) having high affinity (Kd 18 pM) for 125I-iodocyanopindolol (ICYP). Competition binding experiments suggested the presence of more than two subtype of beta-adrenoceptors. 25% of the total population of receptors was found to be of beta 1-type. The remaining 75% represented a mixed population containing what is suggested to be a mixture of beta 2- and atypical beta-adrenoceptors.
Ahmad
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Biotechnology Research Institute, National Reserach Council of Canada, Montréal.
Chrétien
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Daniel
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Shen
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D000317:Adrenergic alpha-Antagonists / Q000378:metabolism; D000420:Albuterol / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000378:metabolism*; D007457:Iodine Radioisotopes; D020120:Iodocyanopindolol; D007545:Isoproterenol / Q000378:metabolism; D010869:Pindolol / Q000031:analogs & derivatives / Q000378:metabolism; D011412:Propanolamines / Q000378:metabolism; D011433:Propranolol / Q000378:metabolism; D011651:Pulmonary Artery; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*
D000317:Adrenergic alpha-Antagonists; D007457:Iodine Radioisotopes; D011412:Propanolamines; D011943:Receptors, Adrenergic, beta; C026777:ICI 118551; D020120:Iodocyanopindolol; D011433:Propranolol; D010869:Pindolol; C025716:ICI 89406; D007545:Isoproterenol; D000420:Albuterol