title: Unexpected sequence diversity in the amino-terminal ends of the coat proteins of strains of sugarcane mosaic virus.
abstract: The sequence of the 3'-terminal 1343 nucleotides of the SC strain of the sugarcane mosaic virus (SCMV-SC) genome was compared with the 1376 nucleotides at the 3' terminus of maize dwarf mosaic virus B (MDMV-B). The SCMV-SC sequence includes an open reading frame which codes for the viral coat protein of 313 amino acids (nucleotides 157 to 1116), followed by a 3' non-coding region of 235 nucleotides and a poly(A) tail. The MDMV-B sequence codes for the capsid protein (nucleotides 157 to 1139) of 328 amino acids and has a 3' non-coding region of 236 nucleotides. The coat protein of SCMV-SC has 92% identity with that of MDMV-B except for the region between amino acid residues 27 and 70 of SCMV-SC. This region of SCMV-SC is smaller (44 residues) than the equivalent region in MDMV-B (59 residues) and has only 22% identity with the MDMV-B sequence. Possible mechanisms for the generation of this sequence diversity are discussed. Despite this diversity, the sequence identities of both the major part of the coat proteins and the 3' non-coding regions confirm the proposal, based on previously described serological data, that SCMV-SC and MDMV-B are strains of SCMV.
authors.affiliation: CSIRO, Division of Biomolecular Engineering, Parkville, Victoria, Australia.
authors.forename: M J
authors.initials: MJ
authors.lastname: Frenkel
authors.forename: J M
authors.initials: JM
authors.lastname: Jilka
authors.forename: N M
authors.initials: NM
authors.lastname: McKern
authors.forename: P M
authors.initials: PM
authors.lastname: Strike
authors.forename: J M
authors.initials: JM
authors.lastname: Clark
authors.forename: D D
authors.initials: DD
authors.lastname: Shukla
authors.forename: C W
authors.initials: CW
authors.lastname: Ward
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D005091:Exons; D014644:Genetic Variation; D007438:Introns; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics*; D016366:Open Reading Frames; D012367:RNA, Viral / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D012367:RNA, Viral