Genetic analysis of ionising radiation sensitive mutants of cultured mammalian cell lines.
The genetic diversity of a range of ionising radiation sensitive mutants of cultured mammalian cell lines has been examined. Hybrids were constructed from suitably marked diploid cells by cell fusion and selected using resistance to HAT and ouabain. Hybrids were examined for ploidy and gamma-ray sensitivity. The data suggest that at least 8 and possibly 9 complementation groups exist which confer sensitivity to ionising radiation. Mutants in at least 3 distinct complementation groups have a reduced ability to rejoin DNA double-strand breaks.
Jeggo
P A
PA
MRC Cell Mutation Unit, University of Sussex, Falmer, Brighton, Great Britain.
Tesmer
J
J
Chen
D J
DJ
D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D006224:Cricetinae; D004247:DNA / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005816:Genetic Complementation Test; D051379:Mice; D009154:Mutation; D011003:Ploidies; D011836:Radiation Tolerance / Q000235:genetics*; D011839:Radiation, Ionizing
D004247:DNA