Deoxycytidine is salvaged not only into DNA but also into phospholipid precursors. III. dCOP-diacylglycerol formation in tonsillar lymphocytes.
In addition to the water-soluble deoxyliponucleotides (Spasokukotskaja et al. (1988), Biochem. Biophys. Res. Commun. 155, 923), a lipid compound was shown to be labeled from external 3H-deoxycytidine (5-3H-CdR) in infant tonsillar lymphocytes. Chlorpromazine enhanced the labeling of this compound, identified by TLC as 3H-dCDP-diacylglycerol (3H-dCOP-DAG). The deoxynucleotide salvage pathway seems to be the main source for dCDP-DAG synthesis, as hydroxyurea increased its labeling from CdR. myo-Inositol induced the disappearance of 5-3H-dCOP-DAG, suggesting its utilization for phosphatidylinositol synthesis. 3H-Arabinosyl-Cytosine (araC) is also incorporated into the lipidic fraction at a rate comparable to its incorporation into DNA, supporting the effect of araC on membrane functions.
Spasokukotskaja
T
T
1st Institute of Biochemistry, Semmelweis University Medical School, Budapest, Hungary.
Taljanidisz
J
J
Sasvári-Székely
M
M
Staub
M
M
D002746:Chlorpromazine / Q000494:pharmacology; D002855:Chromatography, Thin Layer; D003561:Cytarabine / Q000378:metabolism; D003567:Cytidine Diphosphate Diglycerides / Q000096:biosynthesis* / Q000302:isolation & purification; D004247:DNA / Q000096:biosynthesis*; D003841:Deoxycytidine / Q000378:metabolism*; D006801:Humans; D007223:Infant; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D014066:Palatine Tonsil; D010716:Phosphatidylinositols / Q000096:biosynthesis* / Q000302:isolation & purification; D013936:Thymidine / Q000378:metabolism
D003567:Cytidine Diphosphate Diglycerides; D010716:Phosphatidylinositols; D003561:Cytarabine; D003841:Deoxycytidine; D004247:DNA; D002746:Chlorpromazine; D013936:Thymidine