title: Regulation of TRH release by the cultured neonate rat pancreas.
abstract: The TRH secretory responsiveness of the pancreatic islet cell clusters from newborn rat in organ culture was studied. Basal TRH secretion was stable over a 9-day period. The response to various secretagogues was tested on day 4. TRH secretion was stimulated by high potassium-induced depolarization and also through both cAMP and protein kinase-C dependent pathways. Like insulin, TRH release was stimulated by glucose and arginine and inhibited by somatostatin. These data suggest the existence of a common mechanism for TRH and insulin secretion by the pancreatic beta-cells.
authors.affiliation: Laboratoire de Neuroendocrinologie Expérimentale, INSERM U 297, Faculté de Médecine Nord Bd P. Dramard, Marseille, France.
authors.forename: A
authors.initials: A
authors.lastname: Dutour
authors.forename: P
authors.initials: P
authors.lastname: Giraud
authors.forename: J Y
authors.initials: JY
authors.lastname: Maltese
authors.forename: D
authors.initials: D
authors.lastname: Becquet
authors.forename: G
authors.initials: G
authors.lastname: Pesce
authors.forename: P
authors.initials: P
authors.lastname: Salers
authors.forename: L H
authors.initials: LH
authors.lastname: Ouafik
authors.forename: M
authors.initials: M
authors.lastname: Renard
authors.forename: C
authors.initials: C
authors.lastname: Oliver
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D001120:Arginine / Q000494:pharmacology; D003470:Culture Media; D046508:Culture Techniques; D005260:Female; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D011188:Potassium / Q000502:physiology; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013973:Thyrotropin-Releasing Hormone / Q000378:metabolism*
chemical_list: D003470:Culture Media; D007328:Insulin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D013004:Somatostatin; D013973:Thyrotropin-Releasing Hormone; D001120:Arginine; C044258:8-chloro-cyclic adenosine monophosphate; D005947:Glucose; D013755:Tetradecanoylphorbol Acetate; D011188:Potassium