The influence of hypoxic cell-free perfusion and ischemia on cell morphology in the proximal tubular S2-segment of the rat kidney.
In the present study, in-situ perfused rat kidneys were used as a model to demonstrate the different morphological changes induced by various periods of warm ischemia or of warm hypoxic cell-free perfusion. Light and electron microscopic evaluation revealed no changes in the S2 proximal tubular cells after short exposure times of up to 4 min, whereas longer periods resulted in changes ranging from slight alterations (at 10 min) to severe damage (at 60 min). Warm hypoxic cell-free perfusion induced obvious alterations in the proximal tubular cells somewhat sooner than warm ischemia. The microscopical findings were consistent with the statistics of a detailed morphometrical analysis performed on the mitochondrial diameter. The results were further substantiated by counting intramitochondrial electron-dense condensations ('flocculent densities') as indicators of irreversible cell alteration.
Kotowski
A
A
Institut für Anatomie der RWTH Aachen, Federal Republic of Germany.
Ruhrberg
A
A
Kaufmann
P
P
D000818:Animals; D002467:Cell Nucleus / Q000473:pathology; D004848:Epithelium / Q000473:pathology; D000860:Hypoxia / Q000473:pathology*; D007511:Ischemia / Q000473:pathology*; D007668:Kidney / Q000098:blood supply*; D007687:Kidney Tubules, Proximal / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D008871:Microvilli / Q000473:pathology; D008928:Mitochondria / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D014617:Vacuoles / Q000473:pathology