Isolation of cDNAs partially encoding four Xenopus Wnt-1/int-1-related proteins and characterization of their transient expression during embryonic development.
To begin to study the functions of the Wnt-1/int-1 gene family during vertebrate development, we have isolated four Xenopus laevis cDNAs encoding the partial sequence of proteins homologous to Wnt-1/int-1. Xwnt-3, Xwnt-4, Xwnt-5A, and Xwnt-8 demonstrate between 35 and 50% amino acid identity with X. laevis Wnt-1/int-1 and most cysteine residues are conserved. Xwnt-4 and Xwnt-3 transcripts are detected only during the neurula through tadpole stages of development. Expression of Xwnt-8 is observable during gastrulation, declines during neurulation, and is undetectable by the tadpole stage of development. Xwnt-5A transcripts are most prevalent in RNA from oocytes and tadpoles, although low level expression is detected at all stages examined. The temporal changes in expression of these transcripts imply a unique role for each Xwnt during embryogenesis.
Christian
J L
JL
Department of Pharmacology, University of Washington Medical Center, Seattle 98195.
Gavin
B J
BJ
McMahon
A P
AP
Moon
R T
RT
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051153:Wnt Proteins; D051155:Wnt1 Protein; D029867:Xenopus Proteins; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics*; D029961:Zebrafish Proteins
D009841:Oligonucleotides; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; C495249:WNT1 protein, Xenopus; D051153:Wnt Proteins; D051155:Wnt1 Protein; D029867:Xenopus Proteins; D029961:Zebrafish Proteins; C495248:wnt8a protein, Xenopus; D004247:DNA