In vitro inhibition of cell growth of MOLT-4 malignant human T-lymphoblasts by coenzyme F420.
The inhibitory effect of methanogenic coenzymes on the proliferation of MOLT-4 human malignant T-lymphoblasts was tested. Furthermore the effects of methanogenic coenzymes on dihydrofolate reductase activity (DHFR) from chicken liver have been examined. The results showed that heat-stable extracts of the hydrogenotrophs Methanobacterium thermoautotrophicum, Methanoculleus thermophilicum and Methanogenium tationis inhibit both proliferation of human T-lymphoblasts and DHFR activity. Heat-stable extract of the methylotroph Methanosarcina barkeri showed neither inhibitory nor stimulatory effects in both test systems. The present study proves coenzyme F420 to be the active, inhibitory component in methanogenic extracts.
Raemakers-Franken
P C
PC
Department of Microbiology, Faculty of Science, University of Nijmegen, The Netherlands.
De Abreu
R A
RA
Willems
J G
JG
van der Drift
C
C
Vogels
G D
GD
D002455:Cell Division / Q000187:drug effects*; D019605:Euryarchaeota / Q000502:physiology; D005493:Folic Acid Antagonists; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D008727:Methotrexate / Q000494:pharmacology; D011622:Pterins / Q000494:pharmacology; D012256:Riboflavin / Q000031:analogs & derivatives* / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D005493:Folic Acid Antagonists; D011622:Pterins; C015084:coenzyme F420; C036686:methanopterin; D012256:Riboflavin; D008727:Methotrexate