Atrial natriuretic factor recognizes two receptor subtypes in endothelial cells cultured from bovine pulmonary artery.
In this study specific high affinity binding sites for atrial natriuretic factor (rANF(99-126] have been identified on cultured endothelial cells of bovine pulmonary artery origin (BPAEC). A time-dependent rise in cellular cGMP levels stimulated by rANF(99-126) was followed by release of the nucleotide into the incubation medium. The use of truncated, ring-deleted and linear atrial peptide analogs in competitive displacement analysis and measurement of cGMP accumulation indicated that only a minor proportion (5-11%) of the available receptor pool was of the ANF-B receptor subtype, linked to guanylate cyclase, with the remaining major proportion possibly of the ANF-C (clearance) receptor subtype. The existence of two ANF receptor subtypes in this cell culture model would suggest a significant role for the circulating peptide in modulation of pulmonary endothelial cell function, which would influence or complement its direct actions on the underlying vasculature of the pulmonary circulation.
Redmond
E M
EM
Department of Pharmacology, University College Dublin, Ireland.
Cahill
P A
PA
Keenan
A K
AK
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D001667:Binding, Competitive; D002417:Cattle; D002478:Cells, Cultured; D006152:Cyclic GMP / Q000096:biosynthesis; D004705:Endocytosis; D004730:Endothelium, Vascular / Q000378:metabolism*; D006162:Guanylate Cyclase / Q000378:metabolism; D011651:Pulmonary Artery; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000145:classification / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013997:Time Factors
D011956:Receptors, Cell Surface; D009320:Atrial Natriuretic Factor; D006162:Guanylate Cyclase; D017461:Receptors, Atrial Natriuretic Factor; D006152:Cyclic GMP