Immunological and virological characterization of improved construction of recombinant vaccinia virus expressing rinderpest virus hemagglutinin.
We constructed a recombinant vaccinia virus (RVV) expressing rinderpest virus (RPV) hemagglutinin (H) by modifying the promoter region of the original RVV. The promotor region was modified at three points, i.e., an outframe ATG was eliminated, the sequence between the promoter and initiation codon was shortened and the base sequence just upstream of the initiation codon was changed. As compared with the original RVV, the modified RVV was found to produce a remarkably large amount of H protein in infected rabbit kidney cells cultured in vitro and to induce high titers of anti-RPV-H antibodies in rabbits. The median protective doses in rabbits of the modified and of the original RVVs were 10(2) pfu and 10(3.5) pfu, respectively, indicating that the modified RVV was at least 10-times more effective in protection than the original. The neurovirulence of the modified RVV and the parental LC16mO strain was roughly at the same level, and was much lower than that of WR strain. The modified RVV was as heat-stable as the original one. These results indicate that the modified RVV could be a candidate rinderpest vaccine for further examinations including cattle.
Asano
K
K
Fundamental Research Laboratory, Tonen Corporation, Saitama, Japan.
Tsukiyama
K
K
Shibata
S
S
Yamaguchi
K
K
Momoki
T
T
Maruyama
T
T
Kohara
M
M
Miki
K
K
Sugimoto
M
M
Yoshikawa
Y
Y
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D001483:Base Sequence; D001927:Brain Diseases / Q000382:microbiology; D004279:DNA, Viral; D015967:Gene Expression Regulation, Viral; D006389:Hemagglutinins, Viral / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D006358:Hot Temperature; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D011401:Promoter Regions, Genetic / Q000235:genetics; D011817:Rabbits; D012301:Rinderpest / Q000517:prevention & control; D012302:Rinderpest virus / Q000235:genetics / Q000276:immunology*; D012453:Saimiri; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D010948:Viral Plaque Assay; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*; D014774:Virulence
D000914:Antibodies, Viral; D004279:DNA, Viral; D006389:Hemagglutinins, Viral; D014614:Vaccines, Synthetic; D014765:Viral Vaccines