Conglutinin binds the HIV-1 envelope glycoprotein gp 160 and inhibits its interaction with cell membrane CD4.
The highly glycosylated envelope glycoprotein (gp 160) of human immunodeficiency virus (HIV) interacts with the CD4 molecule present on the membrane of CD4+ cells and is involved in the pathobiology of HIV infection. Lectins bind glycoproteins through non-covalent interactions with specific hexose residues. The mammalian C-type lectin bovine conglutinin was examined for its ability to interact with recombinant gp160 (rgp160) produced in vaccinia virus-infected BHK21 cells. Specific binding of conglutinin to rgp160 was demonstrated by ELISA. The interaction of bovine conglutinin with rgp160 was calcium-dependent, which is characteristic of the binding of a C-type lectin to its ligand, and the binding was inhibited in a dose-dependent manner with N-acetyl-D-glucosamine. Deglycosylation of rgp160 abrogated the conglutinin binding. In addition, conglutinin exerted a dose-dependent inhibition of the binding of rgp160 to the CD4 receptor on CEM 13 cells, as demonstrated by FACS analyses. These results indicate that conglutinin may inhibit the infection with HIV-1 through its interaction with the viral envelope glycoprotein.
Andersen
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Department of Medical Microbiology, Odense University, Denmark.
Sørensen
A M
AM
Svehag
S E
SE
Fenouillet
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D000117:Acetylglucosamine / Q000494:pharmacology; D015704:CD4 Antigens / Q000378:metabolism*; D037222:Collectins; D004305:Dose-Response Relationship, Drug; D015686:Gene Products, env / Q000378:metabolism*; D019302:HIV Envelope Protein gp160; D006801:Humans; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000187:drug effects; D011498:Protein Precursors / Q000378:metabolism*; D012712:Serum Globulins / Q000494:pharmacology*; D014759:Viral Envelope Proteins / Q000378:metabolism*
D015704:CD4 Antigens; D037222:Collectins; D015686:Gene Products, env; D019302:HIV Envelope Protein gp160; D008565:Membrane Proteins; D011498:Protein Precursors; D012712:Serum Globulins; D014759:Viral Envelope Proteins; C024014:conglutinin; D000117:Acetylglucosamine