A membrane associated glutamate binding protein from Caenorhabditis elegans and Haemonchus contortus.
1. A glutamate binding protein has been identified in membrane preparations from the free living nematode, Caenorhabditis elegans, and from the parasitic nematode, Haemonchus contortus. 2. This putative glutamate receptor was solubilized with 30 mM octyl-B-glucoside and partially purified by anion exchange and gel filtration chromatography. 3. An 80-fold purification with recovery of 75% of the glutamate binding activity was achieved. 4. The soluble C. elegans binding protein displayed a Kd for glutamate of 0.1 microM, in close agreement with the findings for the membrane associated binding protein. 5. Quisqualate was capable of displacing glutamate from the soluble C. elegans receptor, again in agreement with previous findings for the membrane bound receptor. 6. The fact that a parasitic nematode, Haemonchus contortus, also possesses this putative glutamate receptor, strengthens the case for using C. elegans as a model system for the study of parasitic nematode neuromuscular physiology.
Rohrer
S P
SP
Department of Biochemical Parasitology, Merck, Sharp and Dohme Research Laboratories, Rahway, NJ 07065.
Evans
D V
DV
Bergstrom
A
A
D000818:Animals; D002852:Chromatography, Ion Exchange / Q000379:methods; D003902:Detergents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D006188:Haemonchiasis / Q000378:metabolism*; D007700:Kinetics; D007814:Larva / Q000378:metabolism; D008566:Membranes / Q000378:metabolism; D009348:Nematoda / Q000378:metabolism*; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter / Q000302:isolation & purification* / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate; D014316:Tritium; D014462:Ultrafiltration / Q000379:methods
D003902:Detergents; D005971:Glutamates; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter; D014316:Tritium; D012967:Sodium Dodecyl Sulfate; D018698:Glutamic Acid