Characterization of colonization factor antigen CFA/I from an enterotoxigenic Escherichia coli strain (0128:H12).
CFA/I antigen was isolated and purified from E. coli, mutant 279 B-1-14, serotype 0128:H12, and had the following biochemical and biological features: a) amino-acid content was similar to that of purified antigen prepared from strain H10407; b) latex particles sensitization with purified CFA/I antigen produced bovine and human erythrocytes group A/II hemagglutination in carbohydrates presence; c) purified anti-CFA/I specific antibodies agglutinated CFA/I-positive enterotoxigenic E. coli strains; d) 3H-leucine-labelled CFA/I antigen adhered to rabbits intestinal mucosa at significant values; e) intestinal mucosa pretreating with purified CFA/I antigen, followed by 3H-leucine labelled enterotoxigenic bacteria infection, had a least 3 local effects: 1) intestinal mucosa protection against parental enterotoxigenic bacteria; 2) inhibition of CFA/I-positive bacteria adherence to intestinal mucosa; 3) release of approximately 96% intraluminally inoculated bacteria.
Dima
V F
VF
Cantacuzino Institute, Bucharest, Romania.
Petrovici
A
A
Dima
S V
SV
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D001422:Bacterial Adhesion; D004768:Enterotoxins / Q000096:biosynthesis*; D004926:Escherichia coli / Q000145:classification / Q000276:immunology* / Q000472:pathogenicity / Q000648:ultrastructure; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000276:immunology* / Q000648:ultrastructure; D006386:Hemagglutination Tests; D007114:Immunization; D007421:Intestine, Small / Q000382:microbiology; D008854:Microscopy, Electron; D011817:Rabbits; D012703:Serotyping
D000596:Amino Acids; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D004768:Enterotoxins; C022924:colonization factor antigens; D036781:Fimbriae Proteins