Effect of four heavy metals on the biology of Nostoc muscorum.
This study presents the effects of Cr, Pb, Ni and Ag on growth, pigments, protein, DNA, RNA, heterocyst frequency, uptake of NH4+ and NO3-, loss of electrolytes (Na+ and K+), nitrate reductase and glutamine synthetase activities of Nostoc muscorum. The statistical tests revealed a direct positive correlation between the metal concentration and inhibition of different processes. Ni was found to be more toxic against growth, pigments and heterocyst differentiation compared to the other metals. Inhibition of pigment showed the following trend: chlorophyll greater than phycocyanin greater than carotenoid. No generalized trend for inhibition of macromolecules was observed. The loss of K+ and Na+ as affected by Cr, Ni and Pb was similar but more pronounced for K+ than Na+. The inhibition of physiological variables depicted the following trend: Na+ loss greater than K+ loss greater than glutamine synthetase greater than NH4+ uptake greater than growth greater than NO3- uptake greater than nitrate reductase greater than heterocyst frequency. This study therefore suggests that loss of electrolytes can be used as a first signal of metal toxicity in cyanobacteria. However, further study is needed to confirm whether the abnormality induced by nickel (branch formation) is a physiological or genetic phenomenon.
Rai
L C
LC
Laboratory of Algal Biology, Banaras Hindu University, Varanasi, India.
Raizada
M
M
Mallick
N
N
Husaini
Y
Y
Singh
A K
AK
Dubey
S K
SK
D000641:Ammonia / Q000378:metabolism; D002857:Chromium / Q000633:toxicity; D000458:Cyanobacteria / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000037:antagonists & inhibitors; D007854:Lead / Q000633:toxicity; D008670:Metals / Q000633:toxicity*; D009532:Nickel / Q000633:toxicity; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000037:antagonists & inhibitors; D009566:Nitrates / Q000378:metabolism; D017942:Nitric Acid; D011188:Potassium / Q000378:metabolism; D012834:Silver / Q000633:toxicity; D012964:Sodium / Q000378:metabolism
D008670:Metals; D009566:Nitrates; D002857:Chromium; D007854:Lead; D012834:Silver; D017942:Nitric Acid; D000641:Ammonia; D009532:Nickel; D012964:Sodium; D009565:Nitrate Reductases; D050901:Nitrate Reductase; D005974:Glutamate-Ammonia Ligase; D011188:Potassium