A clinical pregnancy after a simple method of zona cutting, cryopreservation, and zygote intrafallopian transfer.
A 30-year-old nulliparous woman, who was infertile for greater than 3 years, conceived after the procedures of a simple method of zona cutting, cryopreservation, and ZIFT. This case illustrates: (1) acid medium, chymotrypsin, or sucrose are not needed for the procedure of zona cutting; (2) the zygotes resulting from zona cutting survive through freezing and thawing; and (3) oocyte retrieval can be done concomitant with conservative surgery for endometriosis.
Chang
S Y
SY
Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Taipei, Taiwan.
Soong
Y K
YK
Chang
M Y
MY
Lin
P W
PW
Guu
H F
HF
Wang
M L
ML
D000328:Adult; D015925:Cryopreservation; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D009865:Oocytes / Q000166:cytology; D011247:Pregnancy; D012099:Reproductive Techniques; D015384:Zona Glomerulosa / Q000648:ultrastructure*; D015053:Zygote