Delineation of electric and magnetic field effects of extremely low frequency electromagnetic radiation on transcription.
The relative effects of the electric and magnetic field components of extremely low frequency electromagnetic radiation (ELF) on transcription were examined in human leukemia HL-60 cells. Delineation of the individual field contributions was achieved by irradiating cells in separate concentric compartments of a culture dish within a solenoid chamber. This exposure system produced a homogeneous magnetic field with a coincident electric field whose strength varied directly with distance from the center of the culture dish. Irradiation of HL-60 cells with sine wave ELF at 60 Hz and a field strength of 10 Gauss produced a transient increase in the transcriptional rates which reached a maximum of 50-60% enhancement at 30-120 minutes of irradiation and declined to near basal levels by 18 hours. Comparison of transcription responses to ELF of cells in different concentric compartments revealed that the transcriptional effects were primarily the result of the electric field component with little or no contribution from the magnetic field.
Greene
J J
JJ
Department of Biology, Catholic University of America, Washington, DC 20064.
Skowronski
W J
WJ
Mullins
J M
JM
Nardone
R M
RM
Penafiel
M
M
Meister
R
R
D002460:Cell Line; D055590:Electromagnetic Phenomena; D006801:Humans; D007700:Kinetics; D015473:Leukemia, Promyelocytic, Acute; D012322:RNA Precursors / Q000235:genetics / Q000528:radiation effects; D013997:Time Factors; D014158:Transcription, Genetic / Q000528:radiation effects*; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D012322:RNA Precursors; D014316:Tritium; D014529:Uridine