Localization of 2',3'-decycling phosphodiesterases in the Newcastle disease virus virion.
Purified Newcastle disease virus (NDV) virions possess 2',3'-cyclic nucleotide 2'-phosphohydrolase (2'-CNPase) and 2',3'-cyclic nucleotide 3'-phosphohydrolase (3'-CNPase) activities. These enzyme activities cannot be removed from the virion even after extensive purification by chromatography on controlled-pore glass. In the intact virion, the 3'-CNPase activity was stimulated by Triton X-100, while the 2'-CNPase activity was partially inhibited. We have prepared the NDV subunits and have shown that 3'-CNPase was associated exclusively with the viral nucleocapsid. In contrast, the 2'-CNPase activity was associated with both the envelope as well as the nucleocapsid. A threshold amount of both enzyme activities was detected in viral M protein.
Rosenbergová
M
M
Institute of Virology, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Pristasová
S
S
D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism*; D000818:Animals; D002213:Capsid / Q000378:metabolism*; D002642:Chick Embryo; D009522:Newcastle disease virus / Q000201:enzymology*; D010727:Phosphoric Diester Hydrolases; D012260:Ribonucleases / Q000378:metabolism; D014758:Viral Core Proteins / Q000378:metabolism*; D014771:Virion / Q000201:enzymology*
D014758:Viral Core Proteins; D012260:Ribonucleases; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D010727:Phosphoric Diester Hydrolases; C039809:2',3'-cyclic nucleotide 2'-phosphodiesterase; D063309:2',3'-Cyclic Nucleotide 3'-Phosphodiesterase