Functional reconstitution of the hepatic system N amino acid transport activity.
Hepatic System N is responsible for the active plasma-membrane transport of glutamine, histidine and asparagine. This report describes the solubilization and reconstitution of System N activity. Differential solubility resulted in an approximate enrichment of almost 600-fold compared with total cell homogenate. The results indicate that reconstitution can be utilized as a functional assay during purification of the hepatic System N carrier protein.
Tamarappoo
B K
BK
Department of Biochemistry, University of Florida School of Medicine, Gainesville 32610.
Kilberg
M S
MS
D000818:Animals; D001216:Asparagine / Q000378:metabolism*; D001693:Biological Transport, Active; D002462:Cell Membrane / Q000378:metabolism; D003902:Detergents; D005973:Glutamine / Q000378:metabolism*; D006639:Histidine / Q000378:metabolism*; D007700:Kinetics; D008081:Liposomes; D008099:Liver / Q000378:metabolism*; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011092:Polyethylene Glycols; D011510:Proteolipids / Q000378:metabolism; D051381:Rats; D013379:Substrate Specificity
D003902:Detergents; D008081:Liposomes; D008565:Membrane Proteins; D011510:Proteolipids; C020485:proteoliposomes; D005973:Glutamine; D011092:Polyethylene Glycols; D006639:Histidine; D001216:Asparagine