Patterns of neurepithelial cell rearrangement during avian neurulation are determined prior to notochordal inductive interactions.
In the epiblast of elongating primitive-streak-stage avian embryos, MHP cells--short wedge-shaped neurepithelial cells contained within the median hinge point of the bending neural plate--arise from the midline prenodal and nodal area, whereas L cells--tall spindle-shaped neurepithelial cells constituting the lateral neural plate--arise from paired areas flanking the cranial primitive streak. These characteristic differences in neurepithelial cell shape are acquired as a result of inductive interactions with the notochord. Both MHP and L cells undergo extensive rearrangement (intercalation) during shaping and bending of the neural plate, but their pattern of rearrangement differs. MHP cells intercalate with other MHP cells and the population always spans the midline, whereas L cells intercalate with other L cells, remaining in bulk lateral to the midline. The following experiment was performed to establish whether these distinctive rearrangement patterns are determined prior to notochordal inductive interactions. Quail prospective MHP and L cells were transplanted isochronically and heterotopically to chick host blastoderms at stages prior to formation of the notochord (to wit, prospective MHP cells were transplanted into prospective L cell territory and vice versa) and the distribution, fate, and morphological characteristics of grafted cells were determined in chimeras collected 24 hr later. Our results demonstrate that heterotopic MHP and L cells do not adopt the rearrangement pattern characteristic of their new site; rather, they change their position so that grafted MHP cells intermix with MHP cells of the host and grafted L cells intermix with L cells of the host. Thus, patterns of neurepithelial cell rearrangement are determined prior to notochordal inductive interactions. When and how this determination occurs are topics for further studies.
Alvarez
I S
IS
Department of Anatomy, University of Utah School of Medicine, Salt Lake City 84132.
Schoenwolf
G C
GC
D000818:Animals; D001756:Blastoderm / Q000166:cytology*; D002465:Cell Movement; D002490:Central Nervous System / Q000196:embryology*; D002642:Chick Embryo; D002678:Chimera; D004627:Embryonic Induction; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D009672:Notochord / Q000196:embryology*; D011784:Quail / Q000196:embryology