The peptide pyroGlu-Gln-Gly-Ser-Asn, isolated from mouse liver, inhibits growth of rat hepatoma cells in vitro.
The peptide pyroGlu-Gln-Gly-Ser-Asn, recently isolated from mouse liver, inhibited DNA synthesis and proliferation in vitro of MH1C1 cells, a rat clonal strain derived from a Morris transplantable hepatoma. Both the biological peptide isolated from mouse liver and the synthetic homolog showed bell-shaped dose-response curves. Maximal inhibition (approximately 50%) was observed at two separate dose ranges: one at 10(-7)-10(-10) M, and one at 10(-14)-10(-15) M.
Paulsen
J E
JE
Department of Clinical Pharmacology, Rikshospitalet, Oslo, Norway.
Sundby-Hall
K
K
Endresen
L
L
Rugstad
H E
HE
Reichelt
K L
KL
Elgjo
K
K
D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D008099:Liver / Q000737:chemistry / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy / Q000473:pathology*; D051379:Mice; D008969:Molecular Sequence Data; D009368:Neoplasm Transplantation; D009842:Oligopeptides / Q000494:pharmacology*; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D051381:Rats; D013997:Time Factors; D014407:Tumor Cells, Cultured
D009842:Oligopeptides; D010455:Peptides; C067151:pyroglutamyl-glutaminyl-glycyl-seryl-asparagine; D004247:DNA; D011761:Pyrrolidonecarboxylic Acid