title: Induction of Langerhans' cell mitosis in vivo after orchiectomy on mice.
abstract: We recently reported that male mice have fewer Langerhans' cells (LCs) than females and that orchiectomy resulted in an increased LC density. In this study we demonstrate that orchiectomy induces a transient increase in the number of paired LCs (PLCs) before the increase in LC density occurs. The results of double staining for adenosine triphosphatase (ATPase) and 5-bromo-2'-deoxyuridine (BrdU) showed that all examined PLCs had incorporated the intraperitoneally injected BrdU, while only 0.7% of the unpaired LCs were BrdU-positive; that supported the notion of PLCs as being divided daughter LCs. Orchiectomy appears to induce a transient increase in mitotic activity of LCs resulting in the increased LC density.
authors.affiliation: Division of Cell Biology, Fujita Health University, Aichi, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Koyama
authors.forename: S
authors.initials: S
authors.lastname: Nagao
authors.forename: K
authors.initials: K
authors.lastname: Ohashi
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Marunouchi
mesh_terms: D000818:Animals; D002452:Cell Count; D005260:Female; D007150:Immunohistochemistry; D007801:Langerhans Cells / Q000502:physiology*; D008297:Male; D051379:Mice; D008938:Mitosis; D009919:Orchiectomy; D012727:Sex Characteristics; D012867:Skin / Q000166:cytology*