Catecholamine sulfates as internal standards in HPLC determinations of sulfoconjugated catecholamines in plasma and urine.
A method is described to measure catecholamine sulfates from human plasma and urine by isocratic reversed-phase high-performance liquid chromatography with electrochemical detection. For this measurement we use catecholamine 3-sulfate isomers as internal standards and determine the sulfoconjugates only after eliminating the catecholamines. Catecholamines that have previously been used as internal standards are shown to cause a significant overestimation (P less than 0.05) of the catecholamine sulfates--by 10% to 25% and 20% to 42% in human plasma and urine, respectively. The detection limits (signal-to-noise ratio greater than 3) in plasma and urine samples were about 80 pmol/L for each analyte. The intra-assay and interassay CVs were less than 4.0% and 10.6% in human plasma and less than 6.6% and 12.8% in human urine, respectively. The calibration curves for all catecholamine sulfates in human plasma and urine were linear (r greater than 0.96; P less than 0.001) over the respective concentration ranges of 0.1-100 nmol/L and 5-1000 nmol/L.
Strobel
G
G
Department of Pathophysiology and Sports Medicine, University of Heidelberg, F.R.G.
Weicker
H
H
D000310:Adrenal Gland Neoplasms / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004298:Dopamine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D004837:Epinephrine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D006801:Humans; D009638:Norepinephrine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D010673:Pheochromocytoma / Q000097:blood / Q000652:urine; D012015:Reference Standards
C007558:dopamine 3-O-sulfate; C036526:epinephrine sulfate; C017574:noradrenaline sulfate; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine