A rapid, economical, and simple method for concentration of Schistosoma mansoni ova in feces.
An easy, rapid, and economical method for concentration of Schistosoma mansoni ova in feces is described. The basic procedure involves 20 minutes of gravity sedimentation of a fecal suspension sieved through gauze and suspended in a 5% (volume/volume) solution of glycerol in tap water with Ig/L benzoic acid. There is excellent recovery of S. mansoni ova. It can be applied under field conditions and can be implemented by any laboratory with routine facilities. It also allows detection of other ova, larva, and to some extent, cysts.
Shidham
V B
VB
Department of Pathology, Ghamed National Clinic, Baljurashi, Kingdom of Saudi Arabia.
D000818:Animals; D005243:Feces / Q000382:microbiology*; D006801:Humans; D010063:Ovum; D010274:Parasitology / Q000191:economics / Q000379:methods*; D011309:Preservation, Biological; D012550:Schistosoma mansoni / Q000302:isolation & purification*; D013997:Time Factors