Eliminated chromatin of Ascaris contains a gene that encodes a putative ribosomal protein.
Chromatin diminution in the nematodes Parascaris equorum and Ascaris lumbricoides leads to the formation of somatic cells that contain less DNA than the germ-line cells. We present molecular evidence for the coding potential of germ-line-specific DNA. We report on a cDNA clone that codes for a putative ribosomal protein (ALEP-1, for A. lumbricoides eliminated protein 1). That the corresponding gene is located in the eliminated portion of the genome indicates a difference in germ-line and somatic ribosomes of A. lumbricoides and P. equorum. Elimination of the ALEP-1 gene from all somatic cells in its fully active state may represent an alternative way to gene regulation.
Etter
A
A
Institute of Zoology, University of Fribourg, Switzerland.
Aboutanos
M
M
Tobler
H
H
Müller
F
F
D000595:Amino Acid Sequence; D000818:Animals; D001200:Ascaris / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002843:Chromatin / Q000502:physiology*; D015723:Gene Library; D005796:Genes; D006217:Halobacterium / Q000235:genetics; D015801:Helminth Proteins; D008969:Molecular Sequence Data; D012269:Ribosomal Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D002843:Chromatin; D015801:Helminth Proteins; D012269:Ribosomal Proteins; C067677:ALEP-1 protein, Ascaris lumbricoides