title: Prohibitin, an evolutionarily conserved intracellular protein that blocks DNA synthesis in normal fibroblasts and HeLa cells.
abstract: Genes that act inside the cell to negatively regulate proliferation are of great interest because of their implications for such processes as development and cancer, but these genes have been difficult to clone. This report details the cloning and analysis of cDNA for prohibitin, a novel mammalian antiproliferative protein. Microinjection of synthetic prohibitin mRNA blocks entry into S phase in both normal fibroblasts and HeLa cells. Microinjection of an antisense oligonucleotide stimulates entry into S phase. By sequence comparison, the prohibitin gene appears to be the mammalian analog of Cc, a Drosophila gene that is vital for normal development.
authors.affiliation: Laboratory of Molecular Genetics, National Institute on Aging, Baltimore, Maryland 21224.
authors.forename: M J
authors.initials: MJ
authors.lastname: Nuell
authors.forename: D A
authors.initials: DA
authors.lastname: Stewart
authors.forename: L
authors.initials: L
authors.lastname: Walker
authors.forename: V
authors.initials: V
authors.lastname: Friedman
authors.forename: C M
authors.initials: CM
authors.lastname: Wood
authors.forename: G A
authors.initials: GA
authors.lastname: Owens
authors.forename: J R
authors.initials: JR
authors.lastname: Smith
authors.forename: E L
authors.initials: EL
authors.lastname: Schneider
authors.forename: R
authors.initials: R
authors.lastname: Dell' Orco
authors.forename: C K
authors.initials: CK
authors.lastname: Lumpkin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002453:Cell Cycle; D003001:Cloning, Molecular; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D005796:Genes; D006367:HeLa Cells; D006801:Humans; D008099:Liver / Q000502:physiology; D008845:Microinjections; D008969:Molecular Sequence Data; D000091402:Prohibitins; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D012097:Repressor Proteins; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D000091402:Prohibitins; D011506:Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; D004247:DNA