Possible mechanism for the cocarcinogenic effect of bile acids: increased intracellular uptake of methylcholanthrene by C3H/10T1/2 fibroblasts in vitro.
The cocarcinogenic effect of bile acids on the chemical transformation of C3H/10T1/2 fibroblasts was examined in vitro. The assimilation of 3H-methylcholanthrene (3H-MCA) by C3H/10T1/2 cells pretreated with bile acids was examined by the measurement of uptake and by autoradiography. Cells that were pretreated for 48 h with bile acids (100 microM lithocholic acid, 500 microM cholic acid) and then maintained in medium that contained 3H-MCA showed an increase in radioactivity compared to control cells. These results indicate that the transfer of carcinogens into cells is enhanced by pretreatment with bile acids.
Sugezawa
A
A
First Department of Surgery, Tottori University School of Medicine, Yonago, Japan.
Kaibara
N
N
D000818:Animals; D001647:Bile Acids and Salts / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002478:Cells, Cultured; D003043:Cocarcinogenesis; D004247:DNA / Q000096:biosynthesis; D005347:Fibroblasts / Q000187:drug effects; D008748:Methylcholanthrene / Q000493:pharmacokinetics*; D051379:Mice; D008809:Mice, Inbred C3H
D001647:Bile Acids and Salts; D008748:Methylcholanthrene; D004247:DNA