title: Elevated c-myc messenger RNA in multiple myeloma cell lines.
abstract: Oncogene analyses of four human myeloma cell lines provided no indication of gene amplification or rearrangement using DNA probes for the met, raf, abl, mos, erb B, Her-2-neu, fos, myb-7, fms, L-myc, sis, and myb-1 genes. However, a consistent elevation of up to 23-fold in the level of c-myc mRNA was observed in all of the cell lines studied. No restriction fragment length polymorphism (in exons one, two, or three) or c-myc gene amplification has as yet been demonstrated to account for the c-myc mRNA elevation. The c-myc mRNA has a half-life of 25 min which is comparable to that observed in other systems. The elevation in c-myc mRNA is further evidence for the role of the c-myc proto-oncogene in the pathogenesis of myeloma.
authors.affiliation: Department of Medicine, Cross Cancer Institute, Edmonton, Alberta, Canada.
authors.forename: R
authors.initials: R
authors.lastname: Fourney
authors.forename: M
authors.initials: M
authors.lastname: Palmer
authors.forename: A
authors.initials: A
authors.lastname: Ng
authors.forename: K
authors.initials: K
authors.lastname: Dietrich
authors.forename: A
authors.initials: A
authors.lastname: Belch
authors.forename: M
authors.initials: M
authors.lastname: Paterson
authors.forename: L
authors.initials: L
authors.lastname: Brox
mesh_terms: D015342:DNA Probes; D005784:Gene Amplification; D015972:Gene Expression Regulation, Neoplastic; D015321:Gene Rearrangement; D006207:Half-Life; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D009101:Multiple Myeloma / Q000235:genetics / Q000473:pathology*; D009194:Myeloma Proteins / Q000096:biosynthesis / Q000235:genetics; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics*; D009857:Oncogenes; D012150:Polymorphism, Restriction Fragment Length; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis / Q000235:genetics*; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000032:analysis*; D012334:RNA, Neoplasm / Q000032:analysis*; D014407:Tumor Cells, Cultured / Q000737:chemistry
chemical_list: D015342:DNA Probes; D007143:Immunoglobulin Heavy Chains; C000715470:MAS1 protein, human; D009194:Myeloma Proteins; D009363:Neoplasm Proteins; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D012334:RNA, Neoplasm