title: Identification and purification of a protein that binds the yeast ARS consensus sequence.
abstract: We have identified a yeast protein that binds specifically to the ARS consensus sequence. By two-step chromatography we have purified the factor to apparent homogeneity as a single polypeptide of 67 kd. The purified ARS consensus-binding protein (ACBP) recognizes the ARS consensus of the four genomic ARS elements tested, binding preferentially to the T-rich single strand. Point mutations in the consensus significantly reduce the affinity of the single-strand binding. At the histone H4 ARS, ACBP recognizes both the perfect ARS consensus and a 9/11 match 3' of it. These two binding sites correlate with the boundaries of the minimal functional H4 ARS element. A similar configuration of binding sites is found at ARS1. We propose a model implicating this factor in an early step of the initiation of DNA replication.
authors.affiliation: Swiss Institute for Experimental Cancer Research, Lausanne, Switzerland.
authors.forename: J F
authors.initials: JF
authors.lastname: Hofmann
authors.forename: S M
authors.initials: SM
authors.lastname: Gasser
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D005656:Fungal Proteins / Q000302:isolation & purification / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D008957:Models, Genetic; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors
chemical_list: C061886:ABF1 protein, S cerevisiae; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D006657:Histones; D015345:Oligonucleotide Probes; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D004259:DNA-Directed DNA Polymerase