Most somatostatin-immunoreactive neurons in the rat fascia dentata do not contain the calcium-binding protein parvalbumin.
A selective loss of somatostatin (SS)-containing neurons in the hilar region has been reported in patients suffering from temporal lobe epilepsy. Conversely, neurons containing calcium-binding proteins such as parvalbumin (PARV) are known to be very resistant under experimental seizure conditions. In this study, we analyzed the coexistence of SS and PARV in neurons of the rat fascia dentata by using serial semi-thin cryostat sections for pre-embedding immunocytochemistry. Our results show that only 5.7% of the SS-immunoreactive hilar neurons contain PARV. The data suggest that SS-containing hilar neurons are less protected against seizure-induced calcium overload than other neurons containing calcium-binding proteins.
Nitsch
R
R
Institute of Anatomy, University of Freiburg, F.R.G.
Leranth
C
C
Frotscher
M
M
D000818:Animals; D002135:Calcium-Binding Proteins / Q000032:analysis*; D006624:Hippocampus / Q000737:chemistry*; D007124:Immunoenzyme Techniques; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009474:Neurons / Q000737:chemistry*; D010320:Parvalbumins / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000032:analysis*
D002135:Calcium-Binding Proteins; D009419:Nerve Tissue Proteins; D010320:Parvalbumins; D013004:Somatostatin