title: Effect of inhibition of oxygen free radical on ovulation and progesterone production by the in-vitro perfused rabbit ovary.
abstract: The potential role of oxygen free radicals in hCG-induced ovulation was investigated using the free radical scavenging enzymes superoxide dismutase (SOD) and/or catalase with the in-vitro perfused rabbit ovary preparation. SOD (25 micrograms/ml) and SOD + catalase (25 micrograms/ml) significantly reduced the % of large follicles that ovulated during perfusion (P less than 0.005). Neither maturity nor degeneration of ovulated ova and follicular oocytes was affected by SOD and/or catalase. Progesterone concentration in the perfusate was significantly increased in the SOD + catalase treatment group (P less than 0.01). These results indicate a significant role for oxygen free radicals in the process of ovulation.
authors.affiliation: Johns Hopkins University School of Medicine, Department of Gynecology and Obstetrics, Baltimore, Maryland 21205.
authors.forename: T
authors.initials: T
authors.lastname: Miyazaki
authors.forename: K
authors.initials: K
authors.lastname: Sueoka
authors.forename: A M
authors.initials: AM
authors.lastname: Dharmarajan
authors.forename: S J
authors.initials: SJ
authors.lastname: Atlas
authors.forename: G B
authors.initials: GB
authors.lastname: Bulkley
authors.forename: E E
authors.initials: EE
authors.lastname: Wallach
mesh_terms: D000818:Animals; D002374:Catalase / Q000494:pharmacology; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D005260:Female; D005609:Free Radicals; D009924:Organ Culture Techniques; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D010060:Ovulation / Q000187:drug effects / Q000502:physiology*; D010100:Oxygen / Q000378:metabolism*; D010477:Perfusion; D011374:Progesterone / Q000096:biosynthesis*; D011817:Rabbits; D013482:Superoxide Dismutase / Q000494:pharmacology
chemical_list: D006063:Chorionic Gonadotropin; D005609:Free Radicals; D011374:Progesterone; D002374:Catalase; D013482:Superoxide Dismutase; D010100:Oxygen