Detergent (sodium dodecyl sulfate) shock proteins in Escherichia coli.
The protein composition of Escherichia coli W3110 grown in the presence and absence of 5% sodium dodecyl sulfate (SDS) was examined by two-dimensional gel electrophoresis. In SDS-grown cells, at least 4 proteins were turned on, 13 were turned off, 15 were elevated, and 15 were depressed. The 19 unique and elevated SDS-induced spots constituted 7.91% of the total 35S-labeled protein. There was no apparent overlap between these 19 detergent (SDS) stress proteins and those of other known bacterial stress responses. The detergent stress stimulon is a distinct and independent stimulon. Its physiological relevance probably derives from the presence of bile salts in animal gastrointestinal tracts.
Adamowicz
M
M
School of Biological Sciences, University of Nebraska, Lincoln 68588-0343.
Kelley
P M
PM
Nickerson
K W
KW
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008715:Methionine / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D013462:Sulfur Radioisotopes
D001426:Bacterial Proteins; D013462:Sulfur Radioisotopes; D012967:Sodium Dodecyl Sulfate; D008715:Methionine