Retroviral transduction of protein kinase C-gamma into cytotoxic T lymphocyte clones leads to immortalization with retention of specific function.
The molecular pathways that are responsible for delivering the proliferative signals from the cell surface to the nucleus in T lymphocytes are still unresolved, but recent data implicates protein kinase C (PKC) involvement in the TCR signaling pathway. To further address the role of PKC in T cell activation, the effects of high level expression of the PKC-gamma isoenzyme in murine CTL clones were examined. Unlike the parental cells that required periodic Ag stimulation for cell activation and growth, cells expressing a retrovirally transduced PKC-gamma gene propagated in culture independent of the need for Ag stimulation, although maintaining identical functional specificity to the parental CTL. Constitutive PKC-gamma expression may therefore mimic physiologic PKC activation, thereby abrogating the requirement for TCR-Ag interaction in T cell activation.
Finn
O J
OJ
Department of Microbiology and Immunology, Duke University Medical Center, Durham, NC 27710.
Persons
D A
DA
Bendt
K M
KM
Pirami
L
L
Ricciardi
P
P
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002455:Cell Division; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D002999:Clone Cells; D015971:Gene Expression Regulation, Enzymologic; D005822:Genetic Vectors; D007376:Interleukin-2 / Q000502:physiology; D008213:Lymphocyte Activation; D051379:Mice; D008969:Molecular Sequence Data; D011493:Protein Kinase C / Q000235:genetics / Q000502:physiology*; D011994:Recombinant Proteins / Q000235:genetics; D012190:Retroviridae / Q000235:genetics; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology* / Q000201:enzymology; D014161:Transduction, Genetic
D007376:Interleukin-2; D011994:Recombinant Proteins; D011493:Protein Kinase C