title: Stability of liposomes on long term storage.
abstract: The effect of the lipid composition of liposomes on their storage for up to one year under different environmental conditions has been examined using 5,6-carboxyfluorescein as a model drug. When cholesterol and/or alpha-tocopherol were included in the liposomes, a significantly greater amount of dye was retained. The presence of alpha-tocopherol decreased the breakdown of phosphatidylcholine to lysophosphatidylcholine and also reduced the level of peroxidation. Carboxyfluorescein retention was further enhanced when liposomes were stored at 4 degrees C or at room temperature (20 degrees C) in an O2-free atmosphere. Lysophosphatidylcholine formation also slowed when the liposomes were kept at 4 degrees C, or in an O2-free atmosphere. It is concluded that egg yolk lecithin liposomes may be stored for long periods at low temperature in an O2-free atmosphere or with added stabilizers such as cholesterol and alpha-tocopherol.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad de Murcia, Spain.
authors.forename: T
authors.initials: T
authors.lastname: Hernández-Caselles
authors.forename: J
authors.initials: J
authors.lastname: Villalaín
authors.forename: J C
authors.initials: JC
authors.lastname: Gómez-Fernández
mesh_terms: D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002849:Chromatography, Gas; D004355:Drug Stability; D004356:Drug Storage; D005227:Fatty Acids / Q000737:chemistry; D005452:Fluoresceins / Q000737:chemistry; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000737:chemistry; D015227:Lipid Peroxidation; D008081:Liposomes / Q000737:chemistry*; D008244:Lysophosphatidylcholines / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D014810:Vitamin E / Q000737:chemistry
chemical_list: D005227:Fatty Acids; D005452:Fluoresceins; D008041:Linoleic Acids; D008081:Liposomes; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D010743:Phospholipids; D014810:Vitamin E; C024098:6-carboxyfluorescein; D019787:Linoleic Acid