Characterization of adrenergic receptors of the cat iris and nictitating membrane.
Graded pupillary dilations and nictitating membrane (NM) contractions were elicited in anesthetized cats by electrical stimulation of the preganglionic sympathetic nerve or by i.a. administration of norepinephrine (NE) or phenylephrine into the carotid artery. Pupil and NM responses were measured simultaneously from the same side. Alpha-adrenoceptor antagonists were administered intravenously. All of the alpha 1-adrenoceptor blockers tested produced a dose-related reduction of NM responses to both neural and agonist activation; the potency rank order was prazosin greater than WB-4101 greater than phentolamine greater than phenoxybenzamine (PBZ). In contrast, responses of the iris dilator were antagonized only by WB-4101 and PBZ. The iris was almost totally refractory to doses of prazosin and phentolamine that blocked NM responses by more than 75% of control. Neither alpha 2- nor beta-adrenoceptor antagonism produced significant inhibition of neural or agonist activation of either organ (with the exception of high doses of yohimbine on the NM). These results suggest that the postjunctional adrenoceptors of the NM are exclusively of the alpha 1-adrenoceptor subtype. In contrast, those of the iris dilator muscle cannot be easily classified pharmacologically as either alpha 1 or alpha 2-adrenoceptors.
Koss
M C
MC
Department of Pharmacology, University of Oklahoma Health Sciences Center, Oklahoma City.
Hey
J A
JA
Gherezghiher
T
T
D000317:Adrenergic alpha-Antagonists; D000818:Animals; D002415:Cats; D004558:Electric Stimulation; D005260:Female; D007498:Iris / Q000737:chemistry* / Q000187:drug effects; D008297:Male; D009541:Nictitating Membrane / Q000737:chemistry* / Q000187:drug effects; D009638:Norepinephrine; D010656:Phenylephrine; D011680:Pupil / Q000187:drug effects; D011942:Receptors, Adrenergic, alpha / Q000032:analysis / Q000145:classification*; D013564:Sympathetic Nervous System / Q000737:chemistry / Q000187:drug effects
D000317:Adrenergic alpha-Antagonists; D011942:Receptors, Adrenergic, alpha; D010656:Phenylephrine; D009638:Norepinephrine