title: Clostridium bifermentans serovar malaysia: sporulation, biogenesis of inclusion bodies and larvicidal effect on mosquito.
abstract: Sporulation of Clostridium bifermentans serovar malaysia, which has a larvicidal activity towards mosquitoes, was examined by electron microscopy. Parasporal inclusion bodies lacking a crystalline structure were first detected at t5 (5 h after the end of exponentional growth). Also, the presence of "brush-bottle"-like appendages appearing first at t5 was noted; these remained attached to the spores when released after sporangium lysis. Larvicidal activity assayed on Anopheles stephensi larvae appeared at t0 and increased rapidly to a maximum between t5 and t8. However, a decrease in bacterial toxicity occurred with sporangium lysis.
authors.affiliation: Unité des Bactéries Entomopathogènes, Institut Pasteur, Paris.
authors.forename: J F
authors.initials: JF
authors.lastname: Charles
authors.forename: L
authors.initials: L
authors.lastname: Nicolas
authors.forename: M
authors.initials: M
authors.lastname: Sebald
authors.forename: H
authors.initials: H
authors.lastname: de Barjac
mesh_terms: D000818:Animals; D002455:Cell Division / Q000502:physiology; D003013:Clostridium / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D009033:Culicidae; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D013171:Spores, Bacterial / Q000166:cytology / Q000254:growth & development