Lack of effect of age and dietary restriction on DNA single-stranded breaks in brain, liver, and kidney of (C3H x C57BL/10)F1 mice.
Single-stranded DNA breaks in brain, liver, and kidney of (C3H x C57BL/10)F1 mice 6 and 25 months of age on two dietary regimens (isonutrient diets at 85 and 50 kcal/week) were investigated by means of fluorometric analysis of DNA unwinding in alkaline solutions. No age-dependent alterations were found for either control or dietary restricted groups. Except for possible differences for liver in the young age groups, there were no significant differences in the amount of single-stranded DNA breaks between control and dietary restricted mice. These results suggest that the antiaging effects of dietary restriction in rodents are not mediated by a change in the number of accumulated nuclear DNA stranded breaks in internal organs.
Fu
C S
CS
Department of Pathology, University of California, Los Angeles.
Harris
S B
SB
Wilhelmi
P
P
Walford
R L
RL
D000375:Aging; D000818:Animals; D001835:Body Weight; D001921:Brain / Q000033:anatomy & histology; D001923:Brain Chemistry; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000648:ultrastructure*; D004032:Diet; D005260:Female; D005470:Fluorometry; D007668:Kidney / Q000033:anatomy & histology / Q000737:chemistry*; D008099:Liver / Q000033:anatomy & histology / Q000737:chemistry*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009929:Organ Size
D004277:DNA, Single-Stranded