Fucose content of keratan sulphates from bovine articular cartilage.
Alkaline-borohydride-reduced keratan sulphate chains were isolated from bovine articular cartilage (6-8-year-old animals). Nine keratan sulphate fractions of increasing molecular weight were prepared by gel-permeation chromatography on a calibrated column of TSK 30 XL. The samples were analysed for fucose and galactose contents (% by wt. of keratan sulphate) and fucose/galactose ratio. The fucose content increased with molecular size, but the galactose content remained constant. It was concluded that the alpha(1----3)-linked fucose [Thornton, Morris, Cockin, Huckerby, Nieduszynski, Carlstedt, Hardingham & Ratcliffe (1989) Biochem. J. 260, 277-282] was located within the poly-N-acetyl-lactosamine repeat sequence of articular-cartilage keratan sulphate.
Tai
G H
GH
Division of Biological Sciences, University of Lancaster, Bailrigg, U.K.
Brown
G M
GM
Morris
H G
HG
Huckerby
T N
TN
Nieduszynski
I A
IA
D000818:Animals; D001894:Borohydrides; D002241:Carbohydrates / Q000032:analysis; D002358:Cartilage, Articular / Q000737:chemistry*; D002417:Cattle; D002850:Chromatography, Gel; D005643:Fucose / Q000032:analysis*; D005690:Galactose / Q000378:metabolism; D007632:Keratan Sulfate / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight
D001894:Borohydrides; D002241:Carbohydrates; D005643:Fucose; D007632:Keratan Sulfate; D005690:Galactose