Mobilization of the gentamicin resistance gene in Enterococcus faecalis.
Enterococcus faecalis plasmid pBEM10 (a conjugative plasmid encoding beta-lactamase production and gentamicin resistance [Gmr]) was made transfer deficient by using Tn917. Relocation of the Gmr determinant into two sites on pCF10 was observed. Restriction analysis revealed insertion of a common 2.5-kilobase-pair HindIII and a 3.9-kilobase-pair HaeIII fragment encoding Gmr, suggesting that this determinant resides on a transposon similar to Tn4001.
Hodel-Christian
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SL
Program in Infectious Diseases and Clinical Microbiology, University of Texas Medical School, Houston 77030.
Murray
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BE
D015342:DNA Probes; D004251:DNA Transposable Elements / Q000502:physiology; D004352:Drug Resistance, Microbial / Q000235:genetics; D013293:Enterococcus faecalis / Q000235:genetics*; D005798:Genes, Bacterial / Q000502:physiology*; D005839:Gentamicins / Q000494:pharmacology*; D010957:Plasmids / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length
D015342:DNA Probes; D004251:DNA Transposable Elements; D005839:Gentamicins