Prevalence of antibody to heterologous circumsporozoite protein of Plasmodium vivax in Thailand.
The distribution in Thailand of antibody to a recently discovered variant of circumsporozoite proteins of Plasmodium vivax was determined by enzyme-linked immunosorbent assay (ELISA). The ELISA capture antigens were a synthetic peptide of the principal variant sequence ANGAGNQPG and a candidate P vivax vaccine that contained the predominant repeat sequence GDRAA/DGQPA. Serological evidence of recent inoculation with the variant was found throughout Thailand and in migrants from Cambodia, Malaysia, and Burma. IgG antibody to the two P vivax circumsporozoite proteins was detected in 217 of 804 test sera (27%). Within the regions studied the proportion of positive sera specific for the variant epitope ranged from 28% to 66%. A vaccine against the predominant repeat domain may rapidly select for the variant, which already appears to be widespread within Thailand.
Wirtz
R A
RA
Department of Entomology, Walter Reed Army Institute of Research, Washington, DC 20307-5100.
Rosenberg
R
R
Sattabongkot
J
J
Webster
H K
HK
D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008288:Malaria / Q000453:epidemiology / Q000276:immunology; D008297:Male; D008969:Molecular Sequence Data; D010966:Plasmodium vivax / Q000276:immunology*; D013785:Thailand; D014171:Transients and Migrants
D000913:Antibodies, Protozoan