Platelet-derived growth factor blocks the increase in intracellular free Ca2+ caused by calcium ionophores and a volatile anesthetic agent in Swiss 3T3 fibroblasts without altering toxicity.
Platelet-derived growth factor (PDGF) produced an almost complete block of the increase in intracellular free Ca2+ concentration ([Ca2+]i) in Swiss 3T3 fibroblasts caused by the Ca2(+)-selective ionophores 4-bromo-A23187 and ionomycin, and by the volatile anesthetic agent halothane. The effect of PDGF was similar to the decreased [Ca2+]i response to Ca2(+)-ionophores produced by phorbol 12-myristate 13-acetate, an activator of protein kinase C. There was no effect of PDGF or PMA on the acute or delayed toxicity of the Ca2(+)-ionophores to Swiss 3T3 cells, suggesting that the increase in [Ca2+]i is not the direct cause of toxicity of these agents.
Olsen
R A
RA
Department of Pharmacology, Mayo Clinic, Rochester, MN.
Seewald
M J
MJ
Melder
D C
DC
Berggren
M
M
Iaizzo
P A
PA
Powis
G
G
D000331:Aequorin / Q000494:pharmacology; D000001:Calcimycin / Q000031:analogs & derivatives* / Q000633:toxicity; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006221:Halothane / Q000633:toxicity*; D015759:Ionomycin / Q000633:toxicity*; D007476:Ionophores / Q000633:toxicity*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*
D007476:Ionophores; D010982:Platelet-Derived Growth Factor; D000001:Calcimycin; D000331:Aequorin; D015759:Ionomycin; C033170:4-bromo-A-23187; D002118:Calcium; D006221:Halothane