Butyrylcholinesterase and acetylcholinesterase prophylaxis against soman poisoning in mice.
Human butyrylcholinesterase (BChE, EC 3.1.1.8) or acetylcholinesterase (AChE, EC 3.1.1.7) from fetal bovine serum (FBS), administered i.v. in mice, sequestered at approximately 1:1 stoichiometry the highly toxic anti-ChE organophosphate, 1,2,2-trimethylpropyl methyl-fluorophosphonate (soman). A quantitative linear correlation was demonstrated between blood-ChE levels and the protection conferred by exogeneously administered ChE. Results presented here demonstrate that either human BChE or FBS-AChE is an effective prophylactic measure sufficient to protect mice from multiple LD50S of soman without the administration of post-treatment supportive drugs.
Ashani
Y
Y
Israel Institute for Biological Research, Ness-Ziona.
Shapira
S
S
Levy
D
D
Wolfe
A D
AD
Doctor
B P
BP
Raveh
L
L
D000110:Acetylcholinesterase / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D000818:Animals; D002091:Butyrylcholinesterase / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D002800:Cholinesterase Inhibitors / Q000633:toxicity; D004347:Drug Interactions; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011041:Poisoning / Q000517:prevention & control; D011806:Quinolinium Compounds / Q000633:toxicity; D012999:Soman / Q000506:poisoning / Q000633:toxicity*; D013997:Time Factors
D002800:Cholinesterase Inhibitors; D011806:Quinolinium Compounds; C044294:7-((methylethoxyphosphinyl)oxy)-1-methylquinolinium; D012999:Soman; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase