Effect of cholesterol on the ethanol-induced interdigitated gel phase in phosphatidylcholine: use of fluorophore pyrene-labeled phosphatidylcholine.
It is now recognized that many amphiphilic molecules such as ethanol can induce the formation of the fully interdigitated gel phase (L beta I) in phosphatidylcholines (PC's). In the present study, we have developed a simple detection method for the L beta I phase using pyrene-labeled PC (PyrPC), which is a PC analogue with covalently coupled pyrene moiety at the end of one of its acyl chains. The intensity ratio of its fluorescence vibrational bands is a reflection of the polarity of the environment of the fluorophore. We have tested this fluorophore in several established interdigitated lipid systems, including 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (1,2-DPPC) in the presence of high concentrations of ethanol and 1,2-di-O-hexadecyl-sn-glycero-3-phosphocholine (DHPC) and 1,3-dipalmitoyl-sn-glycero-2-phosphocholine (1,3-DPPC) in the absence of any additives. We have found in each of these systems that the ratio of the intensities of band III (387.5 nm) to band I (376.5 nm) is sensitive to the lipid phase change from the noninterdigitated L beta' phase to the interdigitated L beta I phase. By comparison of the III/I ratios for PyrPC in the lipid systems with the III/I ratios for methylpyrene in organic solvents, it was shown that the polarity of the PyrPC environment in the L beta I phase is similar to that of pentanol or ethanol. Using this method, we investigated the effect of cholesterol on the ethanol induction of the interdigitated gel phase in 1,2-DPPC. We found that the ethanol induction of the interdigitated gel phase is prevented by the presence of 20 mol % cholesterol.
Komatsu
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Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City 66103.
Rowe
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D015060:1,2-Dipalmitoylphosphatidylcholine; D002784:Cholesterol; D000431:Ethanol; D005782:Gels; D007202:Indicators and Reagents; D010713:Phosphatidylcholines; D011721:Pyrenes; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D013816:Thermodynamics
D005782:Gels; D007202:Indicators and Reagents; D010713:Phosphatidylcholines; D011721:Pyrenes; D015060:1,2-Dipalmitoylphosphatidylcholine; D000431:Ethanol; D002784:Cholesterol