title: Studies on Clostridium acetobutylicum glnA promoters and antisense RNA.
abstract: The Clostridium acetobutylicum glnA gene has two transcript start sites under the control of promoters p1 and p2. Initiation of transcription was regulated by nitrogen and a downstream region was implicated in the regulation of transcript initiation by nitrogen in Escherichia coli. Putative antisense RNA was produced from a single downstream transcript start site under the control of p3. An up-promoter mutation in p3 resulted in lower levels of glutamine synthetase (GS) activity. Putative antisense RNA had a role in down-regulating GS expression but was not involved in regulation by nitrogen. Deletion of downstream inverted repeat sequences resulted in very low levels of GS activity.
authors.affiliation: Department of Microbiology, University of Cape Town, Rondebosch, South Africa.
authors.forename: P J
authors.initials: PJ
authors.lastname: Janssen
authors.forename: D T
authors.initials: DT
authors.lastname: Jones
authors.forename: D R
authors.initials: DR
authors.lastname: Woods
mesh_terms: D001483:Base Sequence; D003013:Clostridium / Q000201:enzymology / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005974:Glutamate-Ammonia Ligase / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D009584:Nitrogen / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D016372:RNA, Antisense / Q000235:genetics* / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D016372:RNA, Antisense; D005974:Glutamate-Ammonia Ligase; D009584:Nitrogen