Antihypertensive effect of tryptic hydrolysate of milk casein in spontaneously hypertensive rats.
1. Repeated oral administrations of tryptic hydrolysate of bovine milk casein (CEI) showed antihypertensive effect in spontaneously hypertensive rats. 2. Single oral administration of CEI antagonized the pressor response to angiotensin I. 3. Bovine milk casein hydrolysate inhibited the angiotensin I-converting enzyme (ACE) activity. Three peptides with ACE-inhibiting activity were isolated from CEI. 4. It is suggested that ACE-inhibiting peptides in the tryptic hydrolysate milk casein are absorbed from the intestinal tract and produce an antihypertensive effect.
Karaki
H
H
Department of Veterinary Medical Science, Faculty of Agriculture, University of Tokyo, Japan.
Doi
K
K
Sugano
S
S
Uchiwa
H
H
Sugai
R
R
Murakami
U
U
Takemoto
S
S
D000284:Administration, Oral; D000595:Amino Acid Sequence; D000803:Angiotensin I / Q000037:antagonists & inhibitors; D000806:Angiotensin-Converting Enzyme Inhibitors; D000818:Animals; D000959:Antihypertensive Agents; D002364:Caseins / Q000494:pharmacology*; D004562:Electrocardiography / Q000187:drug effects; D006321:Heart / Q000033:anatomy & histology; D006973:Hypertension / Q000188:drug therapy*; D008055:Lipids / Q000097:blood; D008969:Molecular Sequence Data; D009206:Myocardium / Q000473:pathology; D009929:Organ Size / Q000187:drug effects; D011492:Protein Hydrolysates / Q000494:pharmacology*; D051381:Rats; D011918:Rats, Inbred SHR; D014357:Trypsin
D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D002364:Caseins; D008055:Lipids; D011492:Protein Hydrolysates; D000803:Angiotensin I; D014357:Trypsin