title: The gene for 17 beta-hydroxysteroid dehydrogenase maps to human chromosome 17, bands q12-q21, and shows an RFLP with ScaI.
abstract: The gene encoding human 17 beta-hydroxysteroid dehydrogenase (17-HSD; EC 1.1.1.62) is assigned to chromosome 17 by Southern blotting analyses of panels of human x rodent somatic cell hybrids and independently to 17q12-q21 using chromosomal in situ hybridization. A search for physical linkage between 17-HSD and the proto-oncogenes. THRA1 and ERBB2 (both reported to be located in this region of chromosome 17) was performed by pulsed-field gel electrophoresis (PFGE) using several rare-cutting restriction endonucleases. Because all three genes hybridized to DNA fragments of different lengths it seems unlikely that the gene for 17-HSD is located very close to THRA1 and ERBB2. Further evidence for this assumption was obtained from the absence of any coamplification of the 17-HSD gene in 9 breast tumors with amplification of the ERBB2 gene. Analyses of Southern blots of ScaI-digested DNAs from unrelated individuals from Northern Finland revealed a relatively infrequent diallelic restriction fragment length polymorphism, the allele frequencies of which were 0.04 (A1) and 0.96 (A2).
authors.affiliation: Department of Clinical Genetics, Oulu University Central Hospital, Finland.
authors.forename: R
authors.initials: R
authors.lastname: Winqvist
authors.forename: H
authors.initials: H
authors.lastname: Peltoketo
authors.forename: V
authors.initials: V
authors.lastname: Isomaa
authors.forename: K H
authors.initials: KH
authors.lastname: Grzeschik
authors.forename: A
authors.initials: A
authors.lastname: Mannermaa
authors.forename: R
authors.initials: R
authors.lastname: Vihko
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000235:genetics*; D000818:Animals; D015139:Blotting, Southern; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000378:metabolism; D015342:DNA Probes; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D015342:DNA Probes; D004247:DNA; D015067:17-Hydroxysteroid Dehydrogenases; C047020:endodeoxyribonuclease ScaI; D015252:Deoxyribonucleases, Type II Site-Specific