title: Formation and properties of spectrin containing a truncated beta-chain, generated by an endogenous calcium-dependent protease.
abstract: Spectrin chromatographically isolated from human red cell membranes contains a proteolytic activity, inhibited by leupeptin, with a dependence on calcium ions characteristic of a calpain I. The activity accompanies the spectrin on two successive gel filtration columns and is present in both the dimer and the tetramer. It is cryptic in the ghost membrane and in purified membrane skeletons until these are dissociated, apparently because the spectrin is resistant to attack in the tetrameric state. The activity is more strongly expressed in spectrin preparations from young than from old cells. Since the bound enzyme does not detectably change the elution volume of spectrin in gel filtration, it is likely that its molecular weight is low. Its activity reveals itself only in the formation of a modified spectrin, lacking the COOH-terminal, phosphorylated portion of the beta-chain. The phosphorylated fragment (15-20 kDa) can be identified by gel electrophoresis. The proteolytically modified spectrin dimers are unable to associate with like molecules, but are univalent with respect to formation of hybrid tetramers with native spectrin. The association constant for this process is lower than that for self-association of native spectrin by a factor of only 2. The proteolytically modified spectrin behaves similarly in this respect to a truncated spectrin mutant found in a form of hereditary elliptocytosis. The latter also resembles the proteolytic product in that both give rise to the same altered NH2-terminal tryptic fragment from the alpha-chain.
authors.affiliation: Department of Biochemistry, University of Umeå, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Backman
authors.forename: A
authors.initials: A
authors.lastname: Pekrun
authors.forename: W B
authors.initials: WB
authors.lastname: Gratzer
mesh_terms: D001345:Autoradiography; D002118:Calcium / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D013049:Spectrin / Q000096:biosynthesis* / Q000378:metabolism; D014357:Trypsin
chemical_list: D013049:Spectrin; D010450:Endopeptidases; D014357:Trypsin; D002118:Calcium