Oligomeric protein associations: transition from stochastic to deterministic equilibrium.
Transfer of electronic excitation energy (sensitized fluorescence) between donor and acceptor fluorophores separately attached to dimer or tetramer proteins is used to demonstrate the exchange of subunits among the undissociated particles. In dimers subjected to a pressure that produces half-dissociation, the exchange occurs at a rate that approaches the rate of dissociation. In the tetramers of glyceraldehydephosphate dehydrogenase and lactate dehydrogenase at 0 degrees C, the times for subunit exchange are nearly 2 orders of magnitude, and at room temperature 5-10 times longer than the time required to reach the dissociation equilibrium. By application of a novel method, pressure is shown to preferentially increase the rate of dissociation in dimers and decrease the rate of association in tetramers. From these observations, we conclude that the tetramers constitute a heterogeneous population, the members of which are dissociated by pressure according to individual molecular properties that can be retained over periods of time much longer than the time for equilibration of the dissociation. The dissociation of dimers exhibits the characteristics of the classical stochastic chemical equilibria, while those of the tetramers, like the more complex protein aggregates, must already be considered similar to the deterministic mechanical equilibria of macroscopic bodies.
Erijman
L
L
Department of Biochemistry, School of Chemical Sciences, University of Illinois, Urbana 61801.
Weber
G
G
D000818:Animals; D004798:Enzymes / Q000378:metabolism*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D046911:Macromolecular Substances; D008433:Mathematics; D008962:Models, Theoretical; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D013269:Stochastic Processes; D013816:Thermodynamics; D014367:Tryptophan Synthase / Q000378:metabolism
D004798:Enzymes; D046911:Macromolecular Substances; D007770:L-Lactate Dehydrogenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D006593:Hexokinase; D010751:Phosphopyruvate Hydratase; D014367:Tryptophan Synthase