Regulation of TRH release by the cultured neonate rat pancreas.
The TRH secretory responsiveness of the pancreatic islet cell clusters from newborn rat in organ culture was studied. Basal TRH secretion was stable over a 9-day period. The response to various secretagogues was tested on day 4. TRH secretion was stimulated by high potassium-induced depolarization and also through both cAMP and protein kinase-C dependent pathways. Like insulin, TRH release was stimulated by glucose and arginine and inhibited by somatostatin. These data suggest the existence of a common mechanism for TRH and insulin secretion by the pancreatic beta-cells.
Dutour
A
A
Laboratoire de Neuroendocrinologie Expérimentale, INSERM U 297, Faculté de Médecine Nord Bd P. Dramard, Marseille, France.
Giraud
P
P
Maltese
J Y
JY
Becquet
D
D
Pesce
G
G
Salers
P
P
Ouafik
L H
LH
Renard
M
M
Oliver
C
C
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D001120:Arginine / Q000494:pharmacology; D003470:Culture Media; D046508:Culture Techniques; D005260:Female; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D011188:Potassium / Q000502:physiology; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013004:Somatostatin / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013973:Thyrotropin-Releasing Hormone / Q000378:metabolism*
D003470:Culture Media; D007328:Insulin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D013004:Somatostatin; D013973:Thyrotropin-Releasing Hormone; D001120:Arginine; C044258:8-chloro-cyclic adenosine monophosphate; D005947:Glucose; D013755:Tetradecanoylphorbol Acetate; D011188:Potassium