Molecular cloning of the chromosomal breakpoint of a B-cell lymphoma with the t(11;14)(q23;q32) translocation.
The breakpoint of t(11;14)(q23;q32) chromosome translocation in a B-cell lymphoma line, RC-K8, was cloned. Immunoglobulin heavy chain (IGH) constant gene, C gamma 2 at the 5' end, was involved in this translocation, and the DNA segment juxtaposed to the C gamma 2 was proved to be derived from chromosome 11 by somatic cell hybrid study. The normal counterpart of chromosome 11 was also isolated. With a DNA probe near the breakpoint of chromosome 11, Southern blot analysis of RC-K8 and 10 other cases with translocation involving the 11q23 region was conducted, but no rearrangement bands have been observed thus far except for RC-K8.
Akao
Y
Y
Laboratories of Chemotherapy and Immunology, Aichi Cancer Center Research Institute, Nagoya, Japan.
Seto
M
M
Takahashi
T
T
Kubonishi
I
I
Miyoshi
I
I
Nakazawa
S
S
Tsujimoto
Y
Y
Croce
C M
CM
Ueda
R
R
D001483:Base Sequence; D015139:Blotting, Southern; D002880:Chromosomes, Human, Pair 11; D002883:Chromosomes, Human, Pair 14; D003001:Cloning, Molecular; D006801:Humans; D016393:Lymphoma, B-Cell / Q000235:genetics*; D008969:Molecular Sequence Data; D014178:Translocation, Genetic