Nature of the Zn2+ requirement for DNA synthesis by 3T3 cells.
Transit of 3T3 cells from quiescence to S phase requires an adequate supply of Zn2+ during the second half of the transition. The nature of this requirement has been investigated. Completion of the Zn2(+)-dependent process required ongoing mRNA and protein synthesis but could be accomplished in serum-free medium. Combination of low Zn2+ availability with inhibition of mRNA synthesis by 5,6-dichlororibofuranosylbenzimidazole or of protein synthesis by cycloheximide resulted in the cells almost completely reverting to a quiescent state. The results suggest that Zn2+ is required for the accumulation and maintenance of a protein involved in the progression of untransformed cells into S phase.
Chesters
J K
JK
Rowett Research Institute, Bucksburn, Aberdeen, United Kingdom.
Boyne
R
R
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D004261:DNA Replication / Q000502:physiology*; D004004:Dichlororibofuranosylbenzimidazole / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000502:physiology; D014176:Protein Biosynthesis / Q000187:drug effects; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis; D016196:S Phase / Q000502:physiology; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects; D015032:Zinc / Q000502:physiology*
D003470:Culture Media; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D004004:Dichlororibofuranosylbenzimidazole; D007334:Insulin-Like Growth Factor I; D003513:Cycloheximide; D015032:Zinc