Bone morphogenetic protein-2 stimulates alkaline phosphatase activity and collagen synthesis in cultured osteoblastic cells, MC3T3-E1.
The activities of three bone morphogenetic proteins (BMPs), BMP-1, BMP-2 and BMP-3, on alkaline phosphatase activity, collagen synthesis and DNA synthesis were studied in cultured osteoblastic cells, MC3T3-E1. Treatment of cells with BMP-2 for 48 h induces an increase in cellular alkaline phosphatase activity. This stimulatory effect is evident at a concentration as low as 20 ng/ml of BMP-2 and becomes greater with increasing doses of BMP-2. The BMP-2-induced increase in alkaline phosphatase activity is enhanced by the presence of beta-estradiol, dexamethasone or 1 alpha, 25(OH)2D3. BMP-2 and BMP-3 slightly but significantly stimulate collagen synthesis. None of the BMPs stimulates DNA synthesis in MC3T3-E1 cells at doses tested. These results indicate that BMPs act directly on osteoblastic cells and stimulate the expression of the osteoblastic phenotypes.
Takuwa
Y
Y
Department of Internal Medicine, University of Tsukuba, Ibaraki, Japan.
Ohse
C
C
Wang
E A
EA
Wozney
J M
JM
Yamashita
K
K
D000469:Alkaline Phosphatase / Q000378:metabolism*; D019485:Bone Morphogenetic Proteins; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D003907:Dexamethasone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000494:pharmacology; D006133:Growth Substances / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D010006:Osteoblasts / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D011506:Proteins / Q000008:administration & dosage / Q000494:pharmacology*
D019485:Bone Morphogenetic Proteins; D006133:Growth Substances; D011506:Proteins; D004958:Estradiol; D003907:Dexamethasone; D003094:Collagen; D000469:Alkaline Phosphatase