Detection of myosin gene expression in the developing heart using probes derived by polymerase chain reaction.
The polymerase chain reaction provides a rapid method for the molecular cloning of DNA probes suitable for the detection of specific messenger RNA. We have used this approach to prepare probes specific for human cardiac myosin messenger RNA and demonstrate here the use of such probes in the analysis of human cardiac development by hybridization in situ to sections of fetal tissue. This combination of techniques is suitable for the detection of any messenger RNA for which sequence data are available, and offers a powerful new approach to the analysis of cardiac development.
Barton
P J
PJ
Department of Cardiothoracic Surgery, National Heart and Lung Institute, London, U.K.
Moscoso
G
G
Thompson
R P
RP
D005318:Fetal Heart / Q000737:chemistry*; D015870:Gene Expression; D006801:Humans; D009218:Myosins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis*
D012333:RNA, Messenger; D009218:Myosins