Surfactant protein composition of lamellar bodies isolated from rat lung.
Lamellar bodies isolated from rat lung contain all three classes of surfactant proteins, SP-A, SP-B and SP-C, as determined by immunoblot analysis. The amounts of the surfactant proteins present in lamellar bodies, determined by sandwich e.l.i.s.a. (SP-A) and fluorescamine assay (SP-B and SP-C) show that these organelles are highly enriched in the hydrophobic surfactant proteins SP-B and SP-C.
Oosterlaken-Dijksterhuis
M A
MA
Laboratory of Veterinary Biochemistry, Utrecht University, The Netherlands.
van Eijk
M
M
van Buel
B L
BL
van Golde
L M
LM
Haagsman
H P
HP
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000302:isolation & purification; D015151:Immunoblotting; D008168:Lung / Q000737:chemistry* / Q000648:ultrastructure; D008297:Male; D008861:Microsomes / Q000737:chemistry / Q000648:ultrastructure; D008970:Molecular Weight; D015388:Organelles / Q000737:chemistry* / Q000648:ultrastructure; D011510:Proteolipids / Q000302:isolation & purification*; D037662:Pulmonary Surfactant-Associated Protein A; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains
D006023:Glycoproteins; D011510:Proteolipids; D037662:Pulmonary Surfactant-Associated Protein A; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants