title: The nirSTBM region coding for cytochrome cd1-dependent nitrite respiration of Pseudomonas stutzeri consists of a cluster of mono-, di-, and tetraheme proteins.
abstract: Genes for respiratory nitrite reduction (denitrification) of Pseudomonas stutzeri are clustered within 7 kbp. A 4.6-kbp Hind III-Kpn I fragment carrying nirS, the structural gene for cytochrome cd1, was sequenced. An open reading frame immediately downstream of nirS codes for a 22.8-kDa protein with four heme c-binding motifs. Mutagenesis of this gene causes an apparent defect in electron donation to cytochrome cd1. Following this ORF are the structural genes for cytochrome c552, cytochrome c551, and ORF5 that codes for a 11.9-kDa monoheme protein. All cytochromes have a signal sequence for protein export.
authors.affiliation: Lehrstuhl für Mikrobiologie, Universität Karlsruhe, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Jüngst
authors.forename: S
authors.initials: S
authors.lastname: Wakabayashi
authors.forename: H
authors.initials: H
authors.lastname: Matsubara
authors.forename: W G
authors.initials: WG
authors.lastname: Zumft
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003574:Cytochrome c Group; D003580:Cytochromes / Q000235:genetics*; D005798:Genes, Bacterial; D006420:Hemeproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000235:genetics*; D009572:Nitrite Reductases; D011549:Pseudomonas / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D003574:Cytochrome c Group; D003580:Cytochromes; D006420:Hemeproteins; C019315:cytochrome cd1; D009565:Nitrate Reductases; D009572:Nitrite Reductases; D050901:Nitrate Reductase