title: Inositol 1,4,5-triphosphate phosphatase activity in membranes isolated from amphibian skeletal muscle [corrected].
abstract: The hydrolysis of [3H]inositol 1,4,5-trisphosphate by a soluble fraction and by isolated transverse tubule and sarcoplasmic reticulum membranes from frog skeletal muscle was studied. Transverse tubule membranes displayed rates of hydrolysis several-fold higher than those of sacroplasmic reticulum and soluble fraction; Km and Vmax were 25.2 microM and 44.1 nmol/mg/min, respectively. Transverse tubule membranes sequentially hydrolyzed inositol trisphosphate to inositol bisphosphate, inositol 1-phosphate and inositol, indicating that these membranes have inositol bis- and monophosphatases in addition to inositol trisphosphatase.
authors.affiliation: Centro de Estudios Cientificos de Santiago, Casilla, Chile.
authors.forename: X
authors.initials: X
authors.lastname: Sánchez
authors.forename: M A
authors.initials: MA
authors.lastname: Carrasco
authors.forename: J
authors.initials: J
authors.lastname: Vergara
authors.forename: C
authors.initials: C
authors.lastname: Hidalgo
mesh_terms: D000818:Animals; D001001:Anura; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006868:Hydrolysis; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*
chemical_list: D015544:Inositol 1,4,5-Trisphosphate