title: Vascularized bone allografts: in vitro assessment of cell-mediated and humoral responses.
abstract: The immunologic consequences of transplantation of vascularized bone allografts have not been previously characterized. In this study, knee allografts, both vascularized and nonvascularized, were transplanted from Lewis rats to Brown Norway rats across a strong histocompatibility barrier. A total of 66 transplants and 8 control animals were evaluated. The vascularized knee grafts consisted of 1 cm of proximal tibia and distal femur with a minimal muscular cuff isolated on the femoral vessels, and these were transplanted to a heterotopic, subcutaneous position on the abdominal wall of the recipient rat. Nonvascularized allografts (identical but without anastomoses) were transplanted for comparison. The cell-mediated response was measured by lymphocytotoxicity assay, and the humoral response was measured by cytotoxic antibody assay, both employing 51Cr-labeled target cells. The timing and intensity of the immune response differed according to the type of graft. The vascularized bone allografts generated significant cell-mediated and humoral responses as early as 5 days posttransplant. A significant humoral response in nonvascularized bone allografts was not apparent until day 14, while cell-mediated response in these grafts was variable. These findings were correlated with the histologic appearance of the grafted tissue. Cyclosporine, which was administered to one group of vascularized bone allografts, resulted in the suppression of both types of immune responses. The histologic appearance of this group resembled that of isografts transplanted as controls. The clinical application of vascularized bone allografts may offer significant advantages over nonvascularized allografts in the reconstruction of massive bone defects. Complications such as nonunion, fracture, and collapse of articular segments seen in nonvascularized allograft transplantation may be avoided by preservation of the blood supply to the graft. Characterization of the immune response to vascularized bone allografts may subsequently allow the manipulation of the host and/or graft tissue and promote graft incorporation.
authors.affiliation: Department of Orthopedic Surgery, Johns Hopkins University School of Medicine, Baltimore, Md.
authors.forename: P C
authors.initials: PC
authors.lastname: Innis
authors.forename: M A
authors.initials: MA
authors.lastname: Randolph
authors.forename: J P
authors.initials: JP
authors.lastname: Paskert
authors.forename: J F
authors.initials: JF
authors.lastname: Burdick
authors.forename: L W
authors.initials: LW
authors.lastname: Clow
authors.forename: M J
authors.initials: MJ
authors.lastname: Yaremchuk
authors.forename: A J
authors.initials: AJ
authors.lastname: Weiland
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000917:Antibody Formation; D016025:Bone Transplantation / Q000379:methods*; D003524:Cyclosporins / Q000627:therapeutic use; D005360:Fibula / Q000098:blood supply; D006084:Graft Rejection / Q000276:immunology; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D013977:Tibia / Q000098:blood supply / Q000637:transplantation
chemical_list: D003524:Cyclosporins