Flow cytometric detection of jellyfish venom induced cytotoxicity.
Crude venoms of three poisonous jellyfish produce membrane depolarization as determined by the loss of fluorescence intensity of 3,3'-dipentyloxacarbocyanine iodide loaded cells measured by flow cytometry. This method for detecting jellyfish cytotoxicity was reproducible and more sensitive than mouse lethality assays.
Ordóñez
J V
JV
Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore 21201.
Rubinstein
H M
HM
Burnett
J W
JW
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D003064:Cnidarian Venoms / Q000032:analysis* / Q000633:toxicity; D005434:Flow Cytometry; D007700:Kinetics; D051379:Mice
D003064:Cnidarian Venoms