Parentage analysis by endonuclease shattering of hypervariable DNA.
Single-locus DNA probes for tandem repeat sequences are now used in conjunction with particular endonucleases to characterize heritable restriction fragment lengths in parentage tests. Southern blots of this type, however, demonstrate only two attributes of an allele: its length and the presence of nucleotide sequences that are complementary to the probe. Not all restriction fragments of the same apparent length that react with the same probe are identical. Differences between comigrating fragments can be detected by the selection of a restriction enzyme that recognizes sites in a subset of the repeat sequences, and the information content of these loci is therefore increased. This report describes a paternity case in which two brothers appeared, after DNA phenotyping using Hinf I, to be the father. A second phenotyping using Hae III excluded one of the brothers.
Chiafari
F A
FA
Baltimore Rh Typing Laboratory, Inc., Maryland.
Wenk
R E
RE
D000483:Alleles; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000032:analysis / Q000187:drug effects* / Q000235:genetics; D015342:DNA Probes; D004262:DNA Restriction Enzymes / Q000235:genetics*; D004720:Endonucleases / Q000494:pharmacology*; D006580:Genetic Carrier Screening; D006801:Humans; D008297:Male; D010334:Paternity; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length
D015342:DNA Probes; D004247:DNA; D004720:Endonucleases; D004262:DNA Restriction Enzymes