title: Quantal secretion and loss of vesicles induced by veratridine at the crayfish neuromuscular junction.
abstract: Experiments were conducted in nerve-muscle preparations of small young crayfish (Austropotamobius torrentium, Astacus astacus). Application of veratridine in the superfusate induced strong quantal release of transmitter. After about 5 min when quantal release had declined to a low level preparations were fixed for electron microscopy. Unlike control preparations, veratridine-treated preparations revealed nerve terminals which were largely depleted of their synaptic vesicles. Our findings suggest that in the presence of veratridine the decline of quantal secretion results from the loss of vesicles caused by tonic nerve terminal depolarization. Moreover, our results indicate that during or after excessive quantal release triggered by veratridine synaptic vesicles may fuse with both the presynaptic membrane and each other.
authors.affiliation: Pathologisches Institut der Universität Tübingen, F.R.G.
authors.forename: H
authors.initials: H
authors.lastname: Wolburg
authors.forename: A
authors.initials: A
authors.lastname: Beer
authors.forename: W
authors.initials: W
authors.lastname: Finger
mesh_terms: D000818:Animals; D003400:Astacoidea; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D009469:Neuromuscular Junction / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D018377:Neurotransmitter Agents / Q000378:metabolism; D013569:Synapses / Q000502:physiology / Q000648:ultrastructure; D013572:Synaptic Vesicles / Q000187:drug effects / Q000648:ultrastructure*; D014701:Veratridine / Q000494:pharmacology*
chemical_list: D018377:Neurotransmitter Agents; D014701:Veratridine