The stimulatory effect of PDGF on vascular smooth muscle cell migration is mediated by the induction of endogenous basic FGF.
The migration of arterial smooth muscle cells from the media to the intima is a crucial event for the development of the atherosclerotic lesion, and platelet derived growth factor (PDGF) is thought to play an important role in this process. Here we report that the spontaneous migration of bovine smooth muscle (BSM) cells is dependent on endogenously produced basic fibroblast growth factor (bFGF). PDGF stimulates the migration of BSM cells and its effect is abolished by affinity purified anti-bFGF antibody. PDGF induces bFGF mRNA in BSM cells. These results indicate that the effect of PDGF on the migration of BSM cells may be mediated by the induction of endogenous bFGF.
Sato
Y
Y
First Department of Medicine, Medical College of Oita, Japan.
Hamanaka
R
R
Ono
J
J
Kuwano
M
M
Rifkin
D B
DB
Takaki
R
R
D000818:Animals; D000906:Antibodies; D001011:Aorta / Q000187:drug effects / Q000502:physiology; D015152:Blotting, Northern; D002417:Cattle; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D015342:DNA Probes; D016222:Fibroblast Growth Factor 2 / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007700:Kinetics; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics
D000906:Antibodies; D015342:DNA Probes; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2