Caged ATP potentiates guanylate cyclase activity stimulated by atrial natriuretic factor in rat lung membranes.
ATP increases both basal and atrial natriuretic factor (ANF)-stimulated guanylate cyclase activities. The present studies were designed to compare the effect of adenosine-5'-triphosphate, P3-1-(2-nitrophenyl)-ethyl ester (caged ATP), a photolabile derivative of ATP, with ATP. Caged ATP increases both basal and ANF-stimulated enzyme activities in a concentration-dependent manner, but is more potent than ATP. The effect of caged ATP significantly decreases after it is photo-converted to ATP by UV irradiation. These observations suggest that the caged group introduces a hydrophobic interaction thereby facilitating guanylate cyclase activation.
Chang
C H
CH
Department of Medicine, University Hospitals, Cleveland, OH.
Jiang
B
B
Douglas
J G
JG
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D006162:Guanylate Cyclase / Q000378:metabolism*; D008168:Lung / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008566:Membranes / Q000187:drug effects / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains
C472610:atrial natriuretic peptide, rat; C029273:P(3)-1-(2-nitro)phenylethyladenosine 5'-triphosphate; D009320:Atrial Natriuretic Factor; D000255:Adenosine Triphosphate; D006162:Guanylate Cyclase