Growth hormone (GH) secretion in the dwarf rat: release, clearance and responsiveness to GH-releasing factor and somatostatin.
The new mutant GH-deficient dwarf (Dw) rat was used to study the effects of GH-releasing factor (GRF) or somatostatin (SRIF) on GH release. In anaesthetized adult Dw female rats, i.v. injections of GRF (0.031-2.0 micrograms) elicited a dose-dependent release of GH. Although the peak plasma GH responses to maximal GRF doses were much lower in adult Dw rats compared with normal rats of this strain (AS), the responses largely reflected their relative pituitary GH contents (140 +/- 17 micrograms vs 2.9 +/- 0.4 micrograms, AS vs Dw (means +/- S.E.M.), P less than 0.001). Except at 20 days of age, normal AS rats were more sensitive to GRF than Dw rats despite their larger body weight. Peak GH responses to injection of 31.25 ng GRF increased nine-fold in normal rats between 20 and 40 days, whereas the GH responses to this GRF dose diminished in Dw rats over this age range, and their pituitary GH content was only 2-5% of that of age-matched AS rats. Treatment with human GH (200 micrograms/day for 7 days) stimulated growth in 40-day-old Dw rats and slightly increased the GH response to a low dose of GRF. Basal GH levels in adult Dw animals were sevenfold lower than in AS rats (2.4 +/- 0.3 vs 17.6 +/- 3.3 micrograms/l P less than 0.001) and were further suppressed by i.v. infusion of SRIF (25 micrograms/h). As in normal rats, a rebound GH secretion occurred in Dw rats after stopping SRIF, which was blocked by injection of anti-GRF serum.(ABSTRACT TRUNCATED AT 250 WORDS)
Carmignac
D F
DF
Division of Neurophysiology and Neuropharmacology, National Institute for Medical Research, Mill Hill, London.
Robinson
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IC
D000367:Age Factors; D000818:Animals; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005246:Feedback; D005260:Female; D013006:Growth Hormone / Q000032:analysis / Q000097:blood / Q000172:deficiency*; D013007:Growth Hormone-Releasing Hormone / Q000494:pharmacology*; D007262:Infusions, Intravenous; D010902:Pituitary Gland / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011922:Rats, Mutant Strains; D013004:Somatostatin / Q000494:pharmacology*
D013004:Somatostatin; D013006:Growth Hormone; D013007:Growth Hormone-Releasing Hormone