title: Effect of three plastic catheters on survival and growth of Pseudomonas aeruginosa.
abstract: The effect of polyvinylchloride (PVC), polyurethane (PU) and siliconized latex (SL) catheters on the survival and growth of six non-mucoid and three mucoid strains of Pseudomonas aeruginosa was evaluated. Pseudomonas aeruginosa (1 x 10(8)) was incubated in PBS alone (control) or with 30 1-cm length segments of each catheter and the number of viable microorganisms was determined after 8 h, 1, 2, 5, 7 and 10 days. The presence of PVC catheters significantly favoured the survival and growth of non-mucoid strains in comparison to the control (P less than 0.05 at 5 days, P less than 0.01 at 7 days and thereafter); a similar result was observed with SL catheters (P less than 0.05 at 2 days, P less than 0.01 at 5 days and thereafter). No differences were observed with PU catheters. The number of mucoid microorganisms decreased with time in all controls and suspensions containing segments of catheter, but non-mucoid revertants appeared and quickly increased in the presence of PVC and SL (but not PU) catheters. Eluates of PBS previously containing PVC or SL segments induced a 100- to 500-fold increase in the growth of a non-mucoid strain in comparison with PBS alone. It is concluded that some plastic catheters can release substance(s) that favour the viability of P. aeruginosa.
authors.affiliation: Department of Microbiology, School of Medicine, University of Sevilla, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Martínez-Martínez
authors.forename: A
authors.initials: A
authors.lastname: Pascual
authors.forename: E J
authors.initials: EJ
authors.lastname: Perea
mesh_terms: D001422:Bacterial Adhesion; D002408:Catheters, Indwelling / Q000592:standards*; D005069:Evaluation Studies as Topic; D006801:Humans; D011140:Polyurethanes / Q000592:standards*; D011143:Polyvinyl Chloride / Q000592:standards*; D011550:Pseudomonas aeruginosa / Q000254:growth & development*; D012826:Silicone Elastomers / Q000592:standards*
chemical_list: D011140:Polyurethanes; D012826:Silicone Elastomers; D011143:Polyvinyl Chloride