32P-assay of DNA adducts in white blood cells and placentas of pregnant women: lack of residential wood combustion-related adducts but presence of tissue-specific endogenous adducts.
Residential wood combustion (RWC), which has been increasingly used as a heating source, is of health concern because emissions from RWC contain carcinogenic polycyclic aromatic hydrocarbons (PAH). To assess health risk, the possible formation of PAH-DNA adducts in white blood cells (WBC) and placentas of nonsmoking women exposed to RWC smoke during pregnancy was measured by a nuclease P1-enhanced 32P-postlabeling assay having a sensitivity limit of one lesion per 10(9-10) DNA nucleotides. DNA samples isolated from 12 exposed specimens (8 WBC, 4 placentas) and 13 unexposed control specimens (8 WBC, 5 placentas) were hydrolyzed to mononucleotides, which were then 32P-labeled and separated by high-resolution thin-layer chromatography. Comparison of autoradiograms of exposed DNA samples with those of controls failed to show exposure-related adducts. All placental DNA maps exhibited one major (47 +/- 10%) and 12 minor extra spots, however, that were not seen on WBC-DNA maps. These derivatives corresponded to an average of 12 (+/- 6) modifications in 10(9) nucleotides. Similarly, WBC DNA showed four spots that were absent in placental DNA and were not quantified because of their low levels. Neither placental nor WBC DNA adducts coincided chromatographically with the product formed by the reaction of benzo(a)pyrene diol epoxide I with N2 of guanine in DNA. Although these results suggest that RWC smoke does not elicit detectable levels of aromatic DNA adducts in humans, lack of evaluation of RWC exposure levels leaves some uncertainty in this conclusion. The results, however, clearly show that placental and WBC DNA contain covalent modifications that are unrelated to RWC exposure. These DNA derivatives may be caused by ingestion and/or inhalation of, or skin contact with, low levels of environmental genotoxicants or may arise from endogenous electrophiles of as yet unknown origin.
Reddy
M V
MV
Department of Pharmacology, Baylor College of Medicine, Texas Medical Center, Houston.
Kenny
P C
PC
Randerath
K
K
D001564:Benzo(a)pyrene / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D018736:DNA Adducts; D004249:DNA Damage; D005260:Female; D006801:Humans; D006868:Hydrolysis; D007962:Leukocytes / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D010920:Placenta / Q000378:metabolism*; D011083:Polycyclic Compounds / Q000378:metabolism*; D011247:Pregnancy; D012112:Residential Facilities; D012307:Risk Factors; D015719:Single-Strand Specific DNA and RNA Endonucleases; D012906:Smoke / Q000009:adverse effects*; D014934:Wood
D018736:DNA Adducts; D010761:Phosphorus Radioisotopes; D011083:Polycyclic Compounds; D012906:Smoke; C056612:benzo(a)pyrene-DNA adduct; D001564:Benzo(a)pyrene; D004247:DNA; D015719:Single-Strand Specific DNA and RNA Endonucleases