Specific binding of vascular permeability factor to endothelial cells.
Vascular permeability factor (VPF), also known as vascular endothelial cell growth factor, has recently been purified from guinea pig, human, and bovine sources. We show that various fetal or adult endothelial cell strains originating from either capillary or large vessels possess specific high affinity and saturable binding sites for guinea pig tumor-derived [125I]VPF. Two classes of sites with KDs of approximately 10 pM and 1 nM were detected for all endothelial cell types examined. Guinea pig [125I]VPF binding to endothelial cells was inhibited by human VPF (ID50 = 0.8 ng/ml) and by suramin (ID50 = 75 micrograms/ml) but not by heparin. Cross-linking experiments revealed specific [125I]VPF-receptor complexes of two types. Most of the complexes migrated very slowing in SDS-PAGE, indicating that they were of very high molecular weight and probably highly cross-linked. A portion of the molecules migrated as 270 kDa complexes, indicating that the molecular weight of the endothelial cell VPF receptor is about 230 kDa.
Olander
J V
JV
Health Sciences Division, Monsanto Corporate Research, Monsanto Company, St. Louis, Missouri 63167.
Connolly
D T
DT
DeLarco
J E
JE
D000818:Animals; D001667:Binding, Competitive; D002478:Cells, Cultured; D016228:Endothelial Growth Factors / Q000378:metabolism* / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000378:metabolism*; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D008222:Lymphokines / Q000378:metabolism* / Q000494:pharmacology; D011975:Receptors, Mitogen / Q000378:metabolism*; D040262:Receptors, Vascular Endothelial Growth Factor; D013045:Species Specificity; D013498:Suramin / Q000494:pharmacology; D014471:Umbilical Veins; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D008222:Lymphokines; D011975:Receptors, Mitogen; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D013498:Suramin; D006493:Heparin; D040262:Receptors, Vascular Endothelial Growth Factor