title: Relationships between skin prick test, radioallergosorbent test, and chemiluminescent assays in allergic children.
abstract: The results of skin prick tests (SPT), radioallergosorbent tests (RAST), and multiple chemiluminescent tests (DHS-CLA) to grass mix, parietaria, D. farinae, and D. pteronyssinus were evaluated in 43 allergic children. All CLA tests had valid positive and negative control threads. Chemiluminescent assays class 4 matched with RAST class 3 and/or 4 and CLA class 3 with RAST class 2. The D. farinae, D. pteronyssinus, and grass results obtained with CLA, RAST, and SPT were investigated by principal components analysis that showed a good association between different methods of measuring allergenicity. The results of the present study confirm that DHS-CLA is an effective "in vitro" method for the detection of IgE-allergen specific antibody.
authors.affiliation: Pediatric Department, University of Verona, Italy.
authors.forename: S M
authors.initials: SM
authors.lastname: Bonetti
authors.forename: C M
authors.initials: CM
authors.lastname: Bonizzato
authors.forename: G L
authors.initials: GL
authors.lastname: Piacentini
authors.forename: A
authors.initials: A
authors.lastname: Verga
authors.forename: R I
authors.initials: RI
authors.lastname: Harris
authors.forename: A L
authors.initials: AL
authors.lastname: Boner
mesh_terms: D000485:Allergens / Q000276:immunology; D002648:Child; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis* / Q000276:immunology; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000032:analysis*; D008297:Male; D011852:Radioallergosorbent Test; D012882:Skin Tests
chemical_list: D000485:Allergens; D007073:Immunoglobulin E