Does aminotriazole inhibit import of catalase into peroxisomes by retarding unfolding?
Fusion of complementary cell lines from patients with diseases of peroxisome biogenesis leads to peroxisome assembly in the heterokaryons and to uptake of cytosolic catalase by the newly assembled peroxisomes. Here we show that catalase import is inhibited by prior binding to catalase of the inhibitor 3-amino-1,2,4-triazole, which appears to retard unfolding of the protein.
Middelkoop
E
E
E.C. Slater Institute for Biochemical Research, University of Amsterdam, The Netherlands.
Strijland
A
A
Tager
J M
JM
D000640:Amitrole / Q000494:pharmacology*; D001692:Biological Transport; D002374:Catalase / Q000037:antagonists & inhibitors*; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006801:Humans; D008830:Microbodies / Q000187:drug effects* / Q000378:metabolism; D011487:Protein Conformation; D015211:Zellweger Syndrome / Q000378:metabolism
D002374:Catalase; D000640:Amitrole