Defective transcription of the right end of bacteriophage T7 DNA during an abortive infection of F plasmid-containing Escherichia coli.
Transcription of T7 and mutant T3 DNA during infections of F plasmid-containing cells has been analyzed by using Southern hybridization. A transcriptional defect is apparent in these abortively infected cells that is most severe in the class III region of the phage genome. In particular, RNAs that are initiated from the gene 13 promoter are not elongated to give full-length molecules. It is suggested that the transcription defect results from positive supercoiling of the template DNA and that torsional constraints may even prevent the complete entry of the phage genome into an abortively infected cell.
Beck
P J
PJ
Department of Microbiology, University of Texas, Austin 78712.
Molineux
I J
IJ
D015139:Blotting, Southern; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005144:F Factor; D005814:Genes, Viral; D007700:Kinetics; D009154:Mutation; D011401:Promoter Regions, Genetic; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics; D015183:Restriction Mapping; D013604:T-Phages / Q000235:genetics*; D014158:Transcription, Genetic
D004279:DNA, Viral; D012367:RNA, Viral