Identification of the maize chlorotic mottle virus capsid protein cistron and characterization of its subgenomic messenger RNA.
Maize chlorotic mottle virus (MCMV) is a 30-nm icosahedral plant virus composed of a single 25-kDa capsid protein component and a 4.4-kb single-stranded, positive-sense genomic RNA. Northern blot hybridization analysis detected a single 3'-terminal 1.1-kb subgenomic RNA in infected plants. Virion RNA directs the synthesis of several polypeptides in a rabbit reticulocyte lysate in vitro translation system of which only the 25-kDa polypeptide is immunoprecipitated by MCMV capsid protein antiserum. The 1.1-kb subgenomic RNA is a highly efficient messenger RNA for capsid protein synthesis. Positive polarity in vitro transcripts from 3'-proximal MCMV cDNA clones direct the synthesis of the capsid protein in in vitro translation experiments. These data suggest that the MCMV capsid protein is expressed from a subgenomic RNA in vivo, and that the 25-kDa capsid protein is encoded by the 3'-proximal open reading frame in the MCMV genome.
Lommel
S A
SA
Department of Plant Pathology, North Carolina State University, Raleigh 27695-7616.
Kendall
T L
TL
Xiong
Z
Z
Nutter
R C
RC
D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D005814:Genes, Viral; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016366:Open Reading Frames; D010942:Plant Viruses / Q000235:genetics*; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D015678:Viral Structural Proteins / Q000235:genetics*; D003313:Zea mays
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D012367:RNA, Viral; D015678:Viral Structural Proteins