Relationship between depression of HBsAg production and DNA synthesis by interferons in human hepatoma cell line.
The influence of different interferons (IFNs) on HBsAg production and DNA synthesis was studied in PLC/PRF/5 cells using 30 I.U./ml of natural HuIFN-alpha, 25 I.U./ml of recombinant HuIFN-alpha 2, and 5 I.U./ml of natural murine IFN-alpha/beta. All three IFN types inhibited significant inhibitory effect on HBsAg production during the second 24 hr-interval following their addition. After 96 hr HBsAg production had returned to normal levels. Natural HuIFN-alpha clearly depressed cellular DNA synthesis 24 hr after IFN addition which returned to normal within the next 24 hr. Recombinant HuIFN-alpha 2 influenced DNA synthesis only slightly and the mouse IFN-alpha/beta showed no effect.
Hajnická
V
V
Research Institute of Preventive Medicine, Slovak Academy of Sciences, Bratislava.
Stancek
D
D
Fuchsberger
N
N
Kubes
M
M
D006528:Carcinoma, Hepatocellular; D004247:DNA / Q000096:biosynthesis*; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis*; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D008113:Liver Neoplasms; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured
D006514:Hepatitis B Surface Antigens; D007370:Interferon Type I; D011994:Recombinant Proteins; D004247:DNA