Hepatic zonation of insulin-stimulated tyrosine phosphorylation.
Zonal distribution of insulin stimulation of hepatic protein tyrosine phosphorylation, detected by immunoblotting with an anti-phosphotyrosine antibody, has been studied in the in situ perfused rat liver by dual-digitonin-pulse perfusion. Insulin promotes the rapid and sustained tyrosine phosphorylation of two proteins (pp150 and pp69) that are present only in the perivenous hepatocytes, while three others (pp46, pp48 and pp96) are stimulated identically in the periportal and perivenous cells. The ability of insulin to rapidly activate acetyl-CoA carboxylase is indistinguishable between the hepatic zones. Hepatic zonation of insulin-stimulated tyrosine phosphorylation could underly differential hepatic insulin responses and might provide clues to the identification of tyrosine phosphorylated proteins linked to insulin regulation of intracellular events.
Bianchi
A
A
Department of Medicine, Dartmouth Medical School, Hanover, NH 03756.
Quistorff
B
B
Witters
L A
LA
D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D000818:Animals; D003600:Cytosol / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D010477:Perfusion; D010750:Phosphoproteins / Q000302:isolation & purification*; D010766:Phosphorylation; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D014443:Tyrosine
D007328:Insulin; D010750:Phosphoproteins; D011506:Proteins; D014443:Tyrosine; D000103:Acetyl-CoA Carboxylase