title: Aminophospholipid molecular species asymmetry in the human erythrocyte plasma membrane.
abstract: The transbilayer distribution of the molecular species of aminophospholipids in human red blood cell plasma membrane has been investigated using a covalent labelling technique. Separation and quantitative analysis of the molecular species of phosphatidylethanolamine (PE) and phosphatidylserine (PS) was performed using high-performance liquid chromatography with UV detection of the trinitrophenyl derivatives obtained after reaction with trinitrobenzenesulfonic acid (TNBS). When the molecular species distribution obtained with intact cells was compared to that of the whole membrane, a molecular species asymmetry was evident. This phenomenon was most clearly evident when the reaction was performed at low temperatures (0 degrees C) and was obscured by the excessive labelling or probe permeation associated with higher temperatures or longer incubation times. The monoene species were enriched in the outer leaflet, they comprised about 30% of the PE species in this leaflet. The polyunsaturates were preferentially localized in the inner leaflet and this was true of the arachidonyl species in particular as they represented up to 35% of this pool. The w-3 polyunsaturated fatty acids displayed a preferential localization in the plasmalogen subclass in comparison to the diacyl fraction, i.e., they comprised about 58 of the former and 42% of the latter subclass of cellular PE w-3 species. Data concerning the separation, identification and quantification of PS molecular species in human erythrocytes is also presented. The internal localization of the polyunsaturated species as well as the compartmentalization of the w-3 and w-6 pools will have metabolic, structural and physical implications for membrane function.
authors.affiliation: Section of Analytical Chemistry, DICBR, National Institute of Alcohol and Alcohol Abuse, ADAMHA, Bethesda, MD 20892.
authors.forename: F
authors.initials: F
authors.lastname: Hullin
authors.forename: M J
authors.initials: MJ
authors.lastname: Bossant
authors.forename: N
authors.initials: N
authors.lastname: Salem
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004910:Erythrocyte Membrane / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis; D006801:Humans; D010714:Phosphatidylethanolamines / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D010718:Phosphatidylserines / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D013696:Temperature; D014302:Trinitrobenzenesulfonic Acid
chemical_list: D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; D014302:Trinitrobenzenesulfonic Acid