title: Cloning and structure of cDNA encoding alpha-latrotoxin from black widow spider venom.
abstract: cDNA encoding the putative alpha-latrotoxin precursor was isolated from spider venom glands cDNA library and sequenced. The cDNA contained the 4203 base-pair open reading frame corresponding to the 156,855-Da protein composed of 1401 amino acids. Computer analysis of the deduced primary structure revealed the presence of various internal imperfect repeats mainly in its central and C-terminal regions.
authors.affiliation: Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow.
authors.forename: N I
authors.initials: NI
authors.lastname: Kiyatkin
authors.forename: I E
authors.initials: IE
authors.lastname: Dulubova
authors.forename: I A
authors.initials: IA
authors.lastname: Chekhovskaya
authors.forename: E V
authors.initials: EV
authors.lastname: Grishin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015723:Gene Library; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics; D011061:Poly A / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D013111:Spider Venoms / Q000737:chemistry / Q000235:genetics*
chemical_list: D010446:Peptide Fragments; D012333:RNA, Messenger; D013111:Spider Venoms; D011061:Poly A; C020763:alpha-latrotoxin