title: Alcohol induction of ferritin expression in a human hepatoblastoma cell line (HEP G2).
abstract: Hyperferritinemia, an unclear mechanism, is frequently observed in chronic alcoholics. The aim of this work was to study the effect of alcohol on ferritin expression in a human hepatoblastoma cell line, HepG2. This cell line proved to be sensitive to alcohol, since alcohol increased gamma-GT activity both in cells and media. The most striking result was the increase of ferritin in cells and media by alcohol. Moreover, this effect was specific, since it contrasted with a decrease in total protein synthesis and secretion, a decrease in transferrin excretion and a lack of effect on orosomucoid. In our model, alcohol was able to induce, in a specific manner, ferritin expression.
authors.affiliation: INSERM U 49, Unité de Recherches Hépatologiques, Clinique des Maladies du Foie, Rennes, France.
authors.forename: R
authors.initials: R
authors.lastname: Moirand
authors.forename: G
authors.initials: G
authors.lastname: Lescoat
authors.forename: N
authors.initials: N
authors.lastname: Hubert
authors.forename: J F
authors.initials: JF
authors.lastname: Dezier
authors.forename: N
authors.initials: N
authors.lastname: Pasdeloup
authors.forename: P
authors.initials: P
authors.lastname: Brissot
mesh_terms: D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D000431:Ethanol / Q000494:pharmacology*; D005293:Ferritins / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D008113:Liver Neoplasms; D014168:Transferrin / Q000235:genetics; D014407:Tumor Cells, Cultured / Q000187:drug effects*; D005723:gamma-Glutamyltransferase / Q000378:metabolism
chemical_list: D014168:Transferrin; D000431:Ethanol; D005293:Ferritins; D005723:gamma-Glutamyltransferase