title: Cryosectioning of hair follicles. An improved method using liquid nitrogen conduction freezing.
abstract: Horizontal sectioning of scalp biopsies is especially useful in hair diseases characterized by a reduction of follicles in size or number. Horizontal sectioning using standard cryo-microtomes is hampered by the differences in cutting properties between hair follicles and fatty tissue, resulting in loss of topography. We report a simple, inexpensive method to temporarily cool the specimen to an appropriate temperature by means of a conduction system using liquid nitrogen. Immunohistological methods such as immunofluorescence and immunoperoxidase techniques can be applied without restrictions.
authors.affiliation: Department of Dermatology, University of Nijmegen, The Netherlands.
authors.forename: H M
authors.initials: HM
authors.lastname: Van Baar
authors.forename: J
authors.initials: J
authors.lastname: Schalkwijk
authors.forename: C M
authors.initials: CM
authors.lastname: Perret
mesh_terms: D000506:Alopecia Areata / Q000473:pathology; D001706:Biopsy; D005615:Freezing; D006197:Hair / Q000473:pathology*; D006201:Hair Diseases / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry / Q000295:instrumentation*; D009584:Nitrogen; D012535:Scalp / Q000473:pathology
chemical_list: D009584:Nitrogen