Absence of the c-Ha-ras codon 61 point mutation in murine solid tumors induced by subcutaneously applied 7,12-dimethylbenzanthracene.
The c-Ha-ras-1 codon 61 A----T transversion is a highly consistent finding in murine skin DMBA-initiated/TPA-promoted papillomas and carcinomas. We performed Xba I RFLP analysis on DNA from clonal cell lines derived from 11 independent papillomas and a single carcinoma derived from topically applied DMBA and compared this to RFLP on DNA from 7 clonal fibrosarcoma cell lines derived from tumors arising after subcutaneous administration of the same carcinogen. The A----T point mutation was present in all of the papillomas and carcinoma derived by the classic DMBA-TPA protocol but was absent in all of the fibrosarcomas arising after subcutaneous administration of DMBA.
Andrews
D F
DF
Department of Medicine, VA Medical Center, Seattle, WA 98108.
Collins
S J
SJ
Reddy
A L
AL
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000008:administration & dosage / Q000494:pharmacology; D000483:Alleles; D000818:Animals; D001483:Base Sequence; D003062:Codon / Q000187:drug effects*; D005260:Female; D005354:Fibrosarcoma / Q000139:chemically induced / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D011905:Genes, ras / Q000235:genetics*; D006579:Heterozygote; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009154:Mutation; D009374:Neoplasms, Experimental / Q000235:genetics*; D009857:Oncogenes / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D013755:Tetradecanoylphorbol Acetate / Q000008:administration & dosage / Q000494:pharmacology
D003062:Codon; D015127:9,10-Dimethyl-1,2-benzanthracene; D013755:Tetradecanoylphorbol Acetate