Palmitoylated proteins in Ureaplasma urealyticum.
After incubation of Ureaplasma urealyticum serotype 8 in the presence of 3H-labeled palmitic acid, about 25 acylated proteins were detected by electrophoresis and fluorography. Of these, at least six were shown to be antigenic by immunoprecipitation of solubilized palmitate-labeled cells with a homologous polyclonal serum. These six included the serotype 8-specific surface-expressed 96-kDa antigen. After phase partition of palmitate-labeled cells with Triton X-114, all but six acylated proteins partitioned entirely into the detergent phase. The others, including the 96-kDa antigen, partitioned preferentially into the detergent phase and were apparently amphipathic. These results are consistent with the acylated proteins being mainly membrane associated.
Thirkell
D
D
Department of Biochemistry and Microbiology, University of St. Andrews, Fife Scotland.
Myles
A D
AD
Russell
W C
WC
D000215:Acylation; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism; D017830:Octoxynol; D010169:Palmitic Acids / Q000378:metabolism*; D011092:Polyethylene Glycols; D011233:Precipitin Tests; D014509:Ureaplasma / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D008565:Membrane Proteins; D010169:Palmitic Acids; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40