Oxygen radical production during ischemia-reperfusion in the isolated perfused rat liver as monitored by luminol enhanced chemiluminescence.
We have applied the Luminol enhanced chemiluminescence technique to the isolated perfused rat liver during ischemia and reperfusion to monitor the production of oxygen radicals in tissue. Livers under perfusion with Luminol-containing buffer were subjected to 30 minutes of global ischemia followed by 60 minutes of reperfusion. Their chemiluminescence was continuously monitored to obtain the time course of oxygen radical production. Transient bursts of oxygen radical production were observed in the livers as indicated by chemiluminescence changes on reperfusion. Superoxide dismutase treatment abolished while catalase treatment enhanced the reperfusion-induced chemiluminescence transient.
Okuda
M
M
Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia 19104-6089.
Ikai
I
I
Chance
B
B
Kumar
C
C
D000818:Animals; D002374:Catalase / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008163:Luminescent Measurements; D008165:Luminol; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D015427:Reperfusion Injury / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000494:pharmacology
D008165:Luminol; D002374:Catalase; D013482:Superoxide Dismutase; D010100:Oxygen