Changes in thermodependence of the tyrosine transaminating activity in mitochondria of Drosophila melanogaster larvae due to environmental stress.
1. The form of Arrhenius plots of enzyme in mitochondria isolated from Drosophila melanogaster larvae exposed to heat shock, ethanol, or ethanol and heat shock, solubilized with charged detergents was analysed. 2. Heat shock and ethanol caused different changes in membrane microenvironment of the tyrosine transaminating activity, which found expression in different forms of Arrhenius plots, and different values of activation energy of enzyme. 3. The Arrhenius plots of the enzyme from mitochondria of larvae exposed both to ethanol and heat shock, solubilized with charged detergents, were similar to those observed for mitochondria from organisms exposed only to ethanol.
Rzymkiewicz
D M
DM
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa.
D000818:Animals; D004331:Drosophila melanogaster / Q000201:enzymology; D006358:Hot Temperature / Q000009:adverse effects; D007814:Larva / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D013312:Stress, Physiological / Q000201:enzymology; D013816:Thermodynamics; D014444:Tyrosine Transaminase / Q000378:metabolism*
D014444:Tyrosine Transaminase