Dwarf locus mutants lacking three pituitary cell types result from mutations in the POU-domain gene pit-1.
Mutations at the mouse dwarf locus (dw) interrupt the normal development of the anterior pituitary gland, resulting in the loss of expression of growth hormone, prolactin and thyroid-stimulating hormone, and hypoplasia of their respective cell types. Disruptions in the gene encoding the POU-domain transcription factor, Pit-1, occur in both characterized alleles of the dwarf locus. The data indicate that Pit-1 is necessary for the specification of the phenotype of three cell types in the anterior pituitary, and directly link a transcription factor to commitment and progression events in mammalian organogenesis.
Li
S
S
Department of Chemistry, University of California, San Diego, La Jolla 92093.
Crenshaw
E B
EB
Rawson
E J
EJ
Simmons
D M
DM
Swanson
L W
LW
Rosenfeld
M G
MG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004268:DNA-Binding Proteins / Q000235:genetics*; D004393:Dwarfism, Pituitary / Q000235:genetics*; D015870:Gene Expression; D005801:Genes, Homeobox / Q000235:genetics; D013006:Growth Hormone / Q000235:genetics; D051379:Mice; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D012150:Polymorphism, Restriction Fragment Length; D011388:Prolactin / Q000235:genetics; D015183:Restriction Mapping; D050817:Transcription Factor Pit-1; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; D013006:Growth Hormone; D011388:Prolactin; D050817:Transcription Factor Pit-1; D014157:Transcription Factors; C494185:Pou1f1 protein, mouse