Purification and properties of a subtilisin inhibitor and an associated trypsin inhibitor from Dolichos biflorus.
A subtilisin inhibitor and an associated trypsin inhibitor from Dolichos biflorus were purified to homogeneity by conventional methods such as chromatography on DEAE-cellulose, gel filtration on Sephadex G-75, PAGE and affinity chromatography. The final preparations were homogeneous on PAGE. Their pI were 7.66 and 7.70, respectively. The dissociation constant of the complex of the inhibitor with subtilisin was 2.69.10(-10) M. Both the inhibitors were stable to heat, TCA and ethanol. The molecular weights of the subtilisin inhibitor and the associated trypsin inhibitor by gel filtration were 7500 and 8200, respectively.
Bodhe
A M
AM
Division of Biochemical Sciences, National Chemical Laboratory, Pune, India.
D000596:Amino Acids / Q000032:analysis; D001665:Binding Sites / Q000187:drug effects; D007526:Isoelectric Point; D010944:Plants / Q000201:enzymology*; D011480:Protease Inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D012639:Seeds; D013056:Spectrophotometry, Ultraviolet; D013379:Substrate Specificity; D013381:Subtilisins / Q000037:antagonists & inhibitors*; D014361:Trypsin Inhibitors / Q000737:chemistry / Q000302:isolation & purification*
D000596:Amino Acids; D011480:Protease Inhibitors; D014361:Trypsin Inhibitors; D013381:Subtilisins