title: Mutations in the CDP-choline pathway for phospholipid biosynthesis bypass the requirement for an essential phospholipid transfer protein.
abstract: SEC14p is the yeast phosphatidylinositol (PI)/phosphatidylcholine (PC) transfer protein, and it effects an essential stimulation of yeast Golgi secretory function. We now report that the SEC14p localizes to the yeast Golgi and that the SEC14p requirement can be specifically and efficiently bypassed by mutations in any one of at least six genes. One of these suppressor genes was the structural gene for yeast choline kinase (CKI), disruption of which rendered the cell independent of the normally essential SEC14p requirement. The antagonistic action of the CKI gene product on SEC14p function revealed a previously unsuspected influence of biosynthetic activities of the CDP-choline pathway for PC biosynthesis on yeast Golgi function and indicated that SEC14p controls the phospholipid content of yeast Golgi membranes in vivo.
authors.affiliation: Department of Microbiology, University of Illinois, Urbana-Champaign 61801.
authors.forename: A E
authors.initials: AE
authors.lastname: Cleves
authors.forename: T P
authors.initials: TP
authors.lastname: McGee
authors.forename: E A
authors.initials: EA
authors.lastname: Whitters
authors.forename: K M
authors.initials: KM
authors.lastname: Champion
authors.forename: J R
authors.initials: JR
authors.lastname: Aitken
authors.forename: W
authors.initials: W
authors.lastname: Dowhan
authors.forename: M
authors.initials: M
authors.lastname: Goebl
authors.forename: V A
authors.initials: VA
authors.lastname: Bankaitis
mesh_terms: D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D003566:Cytidine Diphosphate Choline / Q000378:metabolism*; D005800:Genes, Fungal; D016153:Genes, Suppressor; D005838:Genotype; D006056:Golgi Apparatus / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism; D049294:Phospholipid Transfer Proteins; D010743:Phospholipids / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D013489:Suppression, Genetic
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D010713:Phosphatidylcholines; D010716:Phosphatidylinositols; D049294:Phospholipid Transfer Proteins; D010743:Phospholipids; D003566:Cytidine Diphosphate Choline