Generation of monoclonal antibodies to murine IL-1 beta and demonstration of IL-1 in vivo.
The role of murine IL-1 beta in vitro and in vivo has not been defined. We describe here the production of neutralizing and immunoprecipitating mAb and polyclonal antibodies specific for murine IL-1 beta and their application to a characterization of the murine IL-1 beta protein. Immunization of either hamsters or rabbits with the recombinant mature form of murine IL-1 beta emulsified in CFA elicited antisera and hamster mAb that only recognized denatured IL-1 beta. In contrast, immunization with rIL-1 beta adsorbed to alum resulted in the generation of neutralizing and immunoprecipitating rabbit and hamster antisera and hamster mAb. All of the mAb recognize both the pro-form of IL-1 beta and the mature bioactive form produced by cultures of murine peritoneal macrophages. Using these antibodies, we demonstrate that approximately half of the IL-1 activity present in supernatants of LPS-treated cultured mouse macrophages is composed of IL-1 beta. Additionally, IL-1 beta as well as IL-1 alpha can be detected in the plasma of LPS-treated mice. These studies, therefore, demonstrate the production of IL-1 beta both in vitro and in vivo.
Hogquist
K A
KA
Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110.
Nett
M A
MA
Sheehan
K C
KC
Pendleton
K D
KD
Schreiber
R D
RD
Chaplin
D D
DD
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D001483:Base Sequence; D004926:Escherichia coli / Q000378:metabolism; D007375:Interleukin-1 / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D007375:Interleukin-1; D011994:Recombinant Proteins