Effects of cytosine methylation at restriction sites on deoxyribonucleic acid (DNA) typing.
The effects of endogenous 5-methylcytosines on deoxyribonucleic acid (DNA) fingerprints were studied. Analysis with methylation-sensitive restriction endonuclease Sau3AI and its methylation-insensitive isoschizomer MboI showed some differences in the patterns generated as a result of 5-methylcytosines at the recognition sites. Moreover, a few bands of sperm DNA did not match those of blood DNA from the same individual, a phenomenon only observed in the digests of methylation-sensitive endonucleases. These findings indicate the unsuitability of methylation-sensitive restriction endonucleases for DNA fingerprinting and other forms of DNA typing, because of the tissue-specific status of the methylation.
Washio
K
K
Department of Legal Medicine, University of Tsukuba, Ibaraki, Japan.
Ueda
S
S
Misawa
S
S
D044503:5-Methylcytosine; D015139:Blotting, Southern; D003596:Cytosine / Q000031:analogs & derivatives* / Q000378:metabolism; D016172:DNA Fingerprinting / Q000379:methods*; D006801:Humans; D008745:Methylation; D012150:Polymorphism, Restriction Fragment Length; D011237:Predictive Value of Tests
D044503:5-Methylcytosine; D003596:Cytosine