title: Antigenic characterization of the nucleoprotein and matrix protein of influenza C virus with monoclonal antibodies.
abstract: Monoclonal antibodies against the nucleoprotein (NP) and matrix (M) protein of influenza C virus were prepared and characterized. At least two non-overlapping or partially overlapping antigenic sites were delineated on each of the proteins by competitive binding assays. Western blot analysis showed that two antigenic sites on the M protein were highly resistant to conformational changes whereas two sites on NP were sensitive. No antigenic variation was seen in either the NP or the M protein when the reactivity of monoclonal antibodies with 23 different influenza C strains isolated over a 41 year period was studied by radioimmunoprecipitation and enzyme-linked immunosorbent assay. Immunofluorescence analysis with the monoclonal antibodies revealed that both the NP and M proteins migrated to the cell nucleus during the replication cycle of influenza C virus.
authors.affiliation: Department of Bacteriology, Yamagata University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sugawara
authors.forename: H
authors.initials: H
authors.lastname: Nishimura
authors.forename: S
authors.initials: S
authors.lastname: Hongo
authors.forename: F
authors.initials: F
authors.lastname: Kitame
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000915:Antibody Affinity; D000936:Antigen-Antibody Complex / Q000032:analysis; D002460:Cell Line; D002642:Chick Embryo; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D009982:Gammainfluenzavirus / Q000276:immunology*; D007700:Kinetics; D009698:Nucleoproteins / Q000032:analysis / Q000276:immunology*; D014763:Viral Matrix Proteins / Q000032:analysis / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D009698:Nucleoproteins; D014763:Viral Matrix Proteins