title: Molecular cloning of the chromosomal breakpoint of a B-cell lymphoma with the t(11;14)(q23;q32) translocation.
abstract: The breakpoint of t(11;14)(q23;q32) chromosome translocation in a B-cell lymphoma line, RC-K8, was cloned. Immunoglobulin heavy chain (IGH) constant gene, C gamma 2 at the 5' end, was involved in this translocation, and the DNA segment juxtaposed to the C gamma 2 was proved to be derived from chromosome 11 by somatic cell hybrid study. The normal counterpart of chromosome 11 was also isolated. With a DNA probe near the breakpoint of chromosome 11, Southern blot analysis of RC-K8 and 10 other cases with translocation involving the 11q23 region was conducted, but no rearrangement bands have been observed thus far except for RC-K8.
authors.affiliation: Laboratories of Chemotherapy and Immunology, Aichi Cancer Center Research Institute, Nagoya, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Akao
authors.forename: M
authors.initials: M
authors.lastname: Seto
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: I
authors.initials: I
authors.lastname: Kubonishi
authors.forename: I
authors.initials: I
authors.lastname: Miyoshi
authors.forename: S
authors.initials: S
authors.lastname: Nakazawa
authors.forename: Y
authors.initials: Y
authors.lastname: Tsujimoto
authors.forename: C M
authors.initials: CM
authors.lastname: Croce
authors.forename: R
authors.initials: R
authors.lastname: Ueda
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D002880:Chromosomes, Human, Pair 11; D002883:Chromosomes, Human, Pair 14; D003001:Cloning, Molecular; D006801:Humans; D016393:Lymphoma, B-Cell / Q000235:genetics*; D008969:Molecular Sequence Data; D014178:Translocation, Genetic