[Oxidation of lactate in isolated rat heart mitochondria].
In unwashed mitochondria the oxidation of L-lactate (with NAD+) proceeds in presence of the added lactate dehydrogenase. The respiration is characterized by the high rate in state 4 and is stimulated by ADP. This process takes place in unwashed mitochondria and homogenate of the heart in absence of added lactate dehydrogenase. Oxidation of lactate with NAD+ is inhibited by rotenone. It has been also revealed that the oxidation of glutamate is insufficiently altered in presence of lactate (with NAD+) in unwashed mitochondria as compared with the washed ones. It is supposed that the stimulating effect of lactate with NAD+ on the mitochondria respiration is not so much a result of the membrane-damaged action as a result of oxidation of lactate dehydrogenase reaction products: phosphorylative oxidation of pyruvate and nonconjugated oxidation of NADH. Utilization of these products takes place in the main respiratory chain, including its first stage.
Kim
N P
NP
Akhmerov
R N
RN
Makhmudov
E S
ES
D000818:Animals; D004492:Edetic Acid; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D066298:In Vitro Techniques; D007773:Lactates / Q000378:metabolism*; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D010766:Phosphorylation; D051381:Rats; D012402:Rotenone / Q000494:pharmacology
D005971:Glutamates; D007773:Lactates; D012402:Rotenone; D009243:NAD; D018698:Glutamic Acid; D004492:Edetic Acid