title: Cloning and sequencing of Lol pI, the major allergenic protein of rye-grass pollen.
abstract: We have isolated a full length cDNA clone encoding the major glycoprotein allergen Lol pI. The clone was selected using a combination of immunological screening of a cDNA expression library and PCR amplification of Lol pI-specific transcripts. Lol pI expressed in bacteria as a fusion protein shows recognition by specific IgE antibodies present in sera of grass pollen-allergic subjects. Northern analysis has shown that the Lol pI transcripts are expressed only in pollen of rye-grass. Molecular cloning of Lol pI provides a molecular genetic approach to study the structure-function relationship of allergens.
authors.affiliation: ImmuLogic Pharmaceutical Corporation, Cambridge, MA 02139.
authors.forename: I J
authors.initials: IJ
authors.lastname: Griffith
authors.forename: P M
authors.initials: PM
authors.lastname: Smith
authors.forename: J
authors.initials: J
authors.lastname: Pollock
authors.forename: P
authors.initials: P
authors.lastname: Theerakulpisut
authors.forename: A
authors.initials: A
authors.lastname: Avjioglu
authors.forename: S
authors.initials: S
authors.lastname: Davies
authors.forename: T
authors.initials: T
authors.lastname: Hough
authors.forename: M B
authors.initials: MB
authors.lastname: Singh
authors.forename: R J
authors.initials: RJ
authors.lastname: Simpson
authors.forename: L D
authors.initials: LD
authors.lastname: Ward
mesh_terms: D000485:Allergens; D000595:Amino Acid Sequence; D052179:Antigens, Plant; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant; D006023:Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins; D011058:Pollen / Q000235:genetics*; D012434:Secale / Q000235:genetics* / Q000276:immunology
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D006023:Glycoproteins; C042814:Lol p I protein, Lolium perenne; D010940:Plant Proteins; D004247:DNA