title: Relationship between depression of HBsAg production and DNA synthesis by interferons in human hepatoma cell line.
abstract: The influence of different interferons (IFNs) on HBsAg production and DNA synthesis was studied in PLC/PRF/5 cells using 30 I.U./ml of natural HuIFN-alpha, 25 I.U./ml of recombinant HuIFN-alpha 2, and 5 I.U./ml of natural murine IFN-alpha/beta. All three IFN types inhibited significant inhibitory effect on HBsAg production during the second 24 hr-interval following their addition. After 96 hr HBsAg production had returned to normal levels. Natural HuIFN-alpha clearly depressed cellular DNA synthesis 24 hr after IFN addition which returned to normal within the next 24 hr. Recombinant HuIFN-alpha 2 influenced DNA synthesis only slightly and the mouse IFN-alpha/beta showed no effect.
authors.affiliation: Research Institute of Preventive Medicine, Slovak Academy of Sciences, Bratislava.
authors.forename: V
authors.initials: V
authors.lastname: Hajnická
authors.forename: D
authors.initials: D
authors.lastname: Stancek
authors.forename: N
authors.initials: N
authors.lastname: Fuchsberger
authors.forename: M
authors.initials: M
authors.lastname: Kubes
mesh_terms: D006528:Carcinoma, Hepatocellular; D004247:DNA / Q000096:biosynthesis*; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis*; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D008113:Liver Neoplasms; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured
chemical_list: D006514:Hepatitis B Surface Antigens; D007370:Interferon Type I; D011994:Recombinant Proteins; D004247:DNA