title: Isolation, identification and synthesis of locustamyotropin (Lom-MT), a novel biologically active insect peptide.
abstract: A peptide that stimulates the spontaneous contractions of the hindgut of Leucophaea maderae has been purified from extracts of brain-corpora cardiaca/corpora allata-subesophageal ganglion complexes of 9000 adult Locusta migratoria and was designated locustamyotropin or Lom-MT. The primary structure of this 12 residue peptide has been determined Gly-Ala-Val-Pro-Ala-Ala-Gln-Phe-Ser-Pro-Arg-Leu-NH2. The C-terminal sequence (Phe-Ser-Pro-Arg-Leu-NH2) is identical to the C-terminal pentapeptide of the pheromone biosynthesis activating neuropeptide, recently isolated from Heliothis zea, and is also similar to the C-terminal of leucopyrokinin of Leucophaea. Synthetic Lom-MT showed biological as well as chemical characteristics, indistinguishable from those of native Lom-MT. In locust preparations, Lom-MT provoked an increase in frequency, amplitude and tonus of contractions of the oviduct, but was inactive in the same conditions on the locust hindgut preparation.
authors.affiliation: Zoological Institute, Katholieke Universiteit Leuven, Belgium.
authors.forename: L
authors.initials: L
authors.lastname: Schoofs
authors.forename: G M
authors.initials: GM
authors.lastname: Holman
authors.forename: T K
authors.initials: TK
authors.lastname: Hayes
authors.forename: A
authors.initials: A
authors.lastname: Tips
authors.forename: R J
authors.initials: RJ
authors.lastname: Nachman
authors.forename: F
authors.initials: F
authors.lastname: Vandesande
authors.forename: A
authors.initials: A
authors.lastname: De Loof
mesh_terms: D000595:Amino Acid Sequence; D000626:Aminopeptidases; D000818:Animals; D001681:Biological Assay; D018826:CD13 Antigens; D002851:Chromatography, High Pressure Liquid; D006110:Grasshoppers / Q000032:analysis*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000032:analysis / Q000138:chemical synthesis / Q000302:isolation & purification*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D009479:Neuropeptides; C065315:locustamyotropin; D000626:Aminopeptidases; D018826:CD13 Antigens