Fluidity and lipid composition of oat and rye shoot plasma membrane: effect of sterol perturbation by xenobiotics.
Oat and rye plants were treated with either tetcyclacis (an experimental plant growth regulator), nuarimol (a fungicide) or gamma-ketotriazole (an experimental herbicide). These treatments reduced shoot growth and changed the lipid composition of the shoot plasma membranes. In oat, both tetcyclacis and nuarimol treatments increased plasma membrane cholesterol and increased the phosphatidylethanolamine/phosphatidylcholine (PE/PC) ratio, whereas gamma-ketotriazole treatment reduced cholesterol and the PE/PC ratio. In rye, all treatments reduced the PE/PC ratio. Generally, the sterol/phospholipid ratio was less in oat than in rye but the cholesterol/phospholipid ratio was greater. With all treatments in oat and rye, increases were observed in unsaturation of the phospholipid acyl chains. The fluidity of membranes was measured by steady-state fluorescence polarisation of the probe diphenylhexatriene; oat membranes were more fluid than rye. Membrane fluidity was greater in plasma membranes from plants treated with the xenobiotics than the controls. The results are discussed in the context of the effect of plasma membrane lipid composition on membrane fluidity, and it is concluded that there appears to be no overall simple relationship between membrane lipid composition and fluidity that holds for all treatments in both species.
Cooke
D T
DT
Department of Agricultural Sciences, University of Bristol, U.K.
Munkonge
F M
FM
Burden
R S
RS
James
C S
CS
D002462:Cell Membrane / Q000737:chemistry; D004161:Diphenylhexatriene; D002523:Edible Grain / Q000378:metabolism*; D005659:Fungicides, Industrial / Q000494:pharmacology; D006540:Herbicides / Q000494:pharmacology; D050356:Lipid Metabolism; D008560:Membrane Fluidity / Q000187:drug effects*; D010743:Phospholipids / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000378:metabolism; D011743:Pyrimidines / Q000494:pharmacology; D012434:Secale / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013261:Sterols / Q000096:biosynthesis*; D014230:Triazoles / Q000494:pharmacology; D015262:Xenobiotics / Q000494:pharmacology*
D005659:Fungicides, Industrial; D006540:Herbicides; D010743:Phospholipids; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011743:Pyrimidines; D013261:Sterols; D014230:Triazoles; D015262:Xenobiotics; D004161:Diphenylhexatriene; C053723:tetcyclacis; C067673:gamma-ketotriazole; C067672:nuarimol