A newly established human acute lymphoblastic leukemia cell line with characteristics of the earliest B-cell maturation.
A new human acute lymphoblastic leukemia (ALL) cell line, designated HBL-3, was established from the bone marrow of a patient with non-T-ALL. The HBL-3 cell line expressed B4 (CD 19), BA-1 (CD 24) and HLA-DR antigens, but not surface immunoglobulin (SIg) or cytoplasmic immunoglobulin (CIg). The cell line lacked the common acute lymphoblastic leukemia antigen (CALLA) and antigenic markers characteristic of T-cell and myeloid cell lineages. The HBL-3 cells had structural rearrangements of both the homologous chromosome 9s, including a translocation with chromosome 1 which has been reported in a patient with common ALL. The cell line had rearranged immunoglobulin heavy chain genes but retained germ-line kappa light chain genes and germ-line T-cell receptor beta- and gamma-chain genes. The HBL-3 cell line was strongly positive for terminal deoxynucleotidyl transferase (TdT). These findings indicate that the HBL-3 cell line is derived from the earliest B-cell committed to B-cell lineage.
Abe
M
M
Department of Pathology, Fukushima Medical College, Japan.
Nakamura
N
N
Fukuhara
S
S
Hayashi
T
T
Kawakami
K
K
Kita
K
K
Kinoshita
T
T
Ohsato
T
T
Wakasa
H
H
D001402:B-Lymphocytes / Q000473:pathology*; D001853:Bone Marrow / Q000473:pathology; D002467:Cell Nucleus / Q000648:ultrastructure; D002648:Child; D002869:Chromosome Aberrations; D002878:Chromosomes, Human, Pair 1; D002893:Chromosomes, Human, Pair 3; D002899:Chromosomes, Human, Pair 9; D003593:Cytoplasm / Q000648:ultrastructure; D004253:DNA Nucleotidylexotransferase / Q000032:analysis; D005260:Female; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D006801:Humans; D016130:Immunophenotyping; D007621:Karyotyping; D008854:Microscopy, Electron; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000473:pathology*; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D004253:DNA Nucleotidylexotransferase