title: Recovery of the neonatal heart after normothermic ischemia. Effect of oxygen and catalase.
abstract: The objective of this study was to determine the effect of oxygen and the oxygen radical-scavenging enzyme catalase on the neonatal rabbit heart exposed to global ischemia. The experiments were performed with an isolated neonatal (7 to 10 days of age) working heart model in which normothermic (37 degrees C) ischemia was produced for 60 minutes. Left ventricular developed pressure, ratio of change of ventricular pressure to change in time, and aortic flow were measured before ischemia and 30 minutes after reperfusing the hearts with physiologic saline solution. In the control group (ischemia only), developed pressure and ratio of change of ventricular pressure to change in time recovered to 27% +/- 3% (mean +/- standard error of the mean) and 24% +/- 7% of baseline; the hearts were incapable of ejecting (aortic flow = 0). Treatment of hearts before and after ischemia with catalase (150 units/ml of perfusate) was studied in a second group (control plus catalase), but functional recovery (developed pressure = 32% +/- 1%; ratio of change of ventricular pressure to change in time = 24% +/- 2%, and aortic flow = 0) was not significantly different from the control group. The effect of washout midway through the ischemic period with a low oxygen (oxygen concentration less than 35 mm Hg) solution was measured in a third group (hypoxic physiologic saline solution). Functional recovery (developed pressure = 13% +/- 3%; ratio of change of ventricular su pressure to change in time = 13% + 2%; aortic flow = 0) was not significantly different from the control and control plus catalase groups. In marked contrast were the effects of washout with an oxygenated (oxygen concentration greater than 500 mm Hg) solution (oxygenated physiologic saline solution) in which functional recovery (developed pressure = 78% +/- 3%; ratio of change of ventricular pressure to change in time = 80% +/- 3%; aortic flow = 39% +/- 9%) was significantly better than in the control, control plus catalase, and hypoxic physiologic saline solution groups. Use of modified St. Thomas' Hospital cardioplegic solution (cardioplegic solution group) during the ischemic period also resulted in substantial functional recovery (developed pressure = 80% +/- 3%; ratio of change of ventricular pressure to change in time = 78% +/- 5%; aortic flow = 64% +/- 7%) that did not differ significantly from that in the oxygenated physiologic saline solution group.(ABSTRACT TRUNCATED AT 400 WORDS)
authors.affiliation: Department of Surgery, University of Michigan Medical School, Ann Arbor.
authors.forename: T N
authors.initials: TN
authors.lastname: Zweng
authors.forename: M J
authors.initials: MJ
authors.lastname: Lynch
authors.forename: E L
authors.initials: EL
authors.lastname: Bove
authors.forename: M H
authors.initials: MH
authors.lastname: Fox
authors.forename: M D
authors.initials: MD
authors.lastname: Iannettoni
authors.forename: S F
authors.initials: SF
authors.lastname: Bolling
authors.forename: K P
authors.initials: KP
authors.lastname: Gallagher
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D002314:Cardioplegic Solutions; D002374:Catalase / Q000494:pharmacology*; D003326:Coronary Circulation; D016166:Free Radical Scavengers; D006321:Heart / Q000503:physiopathology*; D015425:Myocardial Reperfusion; D010100:Oxygen; D011817:Rabbits; D012965:Sodium Chloride; D013696:Temperature; D016277:Ventricular Function, Left
chemical_list: D002314:Cardioplegic Solutions; D016166:Free Radical Scavengers; D012965:Sodium Chloride; D002374:Catalase; D010100:Oxygen