Solution behaviour of Chromobacter viscosum and Pseudomonas sp. lipases. No evidence of self-association.
1. The size of two bacterial lipases was studied by SDS/PAGE, sedimentation velocity and sedimentation equilibrium to test for possible self-association behaviour. 2. Mr values of selected lipases were obtained from SDS/PAGE and sedimentation-velocity measurements, together with an absolute determination by sedimentation equilibrium 3. The Mr values obtained in a variety of aqueous solvents indicate that lipases do not self-associate in solution, suggesting the absence of surface hydrophobic patches.
Simpkin
N J
NJ
Department of Applied Biochemistry and Food Science, University of Nottingham, Sutton Bonington, U.K.
Harding
S E
SE
Tombs
M P
MP
D002861:Chromobacterium / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D008049:Lipase / Q000302:isolation & purification / Q000378:metabolism*; D046911:Macromolecular Substances; D008970:Molecular Weight; D011549:Pseudomonas / Q000201:enzymology*; D012996:Solutions
D046911:Macromolecular Substances; D012996:Solutions; D008049:Lipase