Ultrastructural changes in the abdominal midgut of the mosquito, Culiseta melanura, during the gonotrophic cycle.
Abdominal midguts of the mosquito, Culiseta melanura, were examined by light and electron microscopy 1 hr-14 days days after blood feeding. Epithelial cells were drastically altered from columnar to squamous in form after engorgement, and returned to columnar by day 4 after feeding. Accumulation of mitochondria along brush borders of digestive cells, followed by the appearance of large secondary lysosomes, accompanied blood digestion. Evidence was obtained that myelin-like material in the lysosomes, probably the result of mitochondrial autolysis, is extruded into the lumen. Digestive cells resumed their pre-blood meal appearance by 10-14 days post-engorgement. Regenerative cells were scattered throughout the basal portion of the epithelium, along with endocrine cells. Other midgut cells containing large, microvilli-lined apical cavities were identified in most specimens. No evidence of division or differentiation was obtained for any cell types.
Weaver
S C
SC
Department of Entomology, University of Maryland, College Park 20742.
Scott
T W
TW
D000818:Animals; D009033:Culicidae / Q000502:physiology / Q000648:ultrastructure*; D004064:Digestive System / Q000648:ultrastructure; D004847:Epithelial Cells; D004848:Epithelium / Q000648:ultrastructure; D005247:Feeding Behavior / Q000502:physiology*; D006651:Histocytochemistry; D008247:Lysosomes / Q000648:ultrastructure; D008854:Microscopy, Electron; D008871:Microvilli / Q000648:ultrastructure; D008928:Mitochondria / Q000648:ultrastructure; D013997:Time Factors