Predominance of type III over type I cell surface procollagens in rat lung fibroblasts.
Binding assays of 125I-labeled affinity-purified monospecific type I and III procollagen antibodies to fibroblasts have revealed the presence of type I and III procollagens on the cell surface, and that the amount of type III procollagen is 10 times greater than that of type I procollagen. Analyses by flow cytometry and fluorescent microscopy after treatment with an isothiocyanate (FITC)-conjugated second antibody supported this finding.
Nagai
Y
Y
Department of Biochemistry, College of Medicine, University of Vermont, Burlington 05405.
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000032:analysis; D002462:Cell Membrane / Q000737:chemistry; D002478:Cells, Cultured; D005333:Fetus; D005347:Fibroblasts / Q000737:chemistry; D005434:Flow Cytometry; D008168:Lung / Q000737:chemistry*; D011347:Procollagen / Q000032:analysis* / Q000276:immunology; D051381:Rats
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D011347:Procollagen