Comparative studies of volume restoration following cold-stress induced swelling in renal tissues--II. Effects of furosemide, amiloride and K+ permeability in rabbit cortex slices.
1. Inhibition of volume restoration by ouabain in rabbit kidney cortex slices is related to an increase in Na+ concentration which may reach levels higher than equilibrium with the external medium. 2. The uphill accumulation of Na+ is not sensitive to amiloride and furosemide. It is dependent on the level of K+ in the external medium. 3. Bringing the slices from cold to normal temperature induces a large increase in Rb86 efflux. 4. It is concluded that the Na+ entry inhibiting volume restoration is driven by an increase in K+ expel occurring upon tissue rewarming.
Ouahbi
A
A
Laboratory of animal physiology, University of Liège, Belgique.
Gilles
R
R
D000584:Amiloride / Q000494:pharmacology*; D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects; D003080:Cold Temperature; D005665:Furosemide / Q000494:pharmacology*; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D007700:Kinetics; D008297:Male; D011188:Potassium / Q000378:metabolism*; D011817:Rabbits; D012964:Sodium / Q000378:metabolism; D013312:Stress, Physiological
D000584:Amiloride; D005665:Furosemide; D012964:Sodium; D011188:Potassium