The leader sequence of streptokinase is responsible for its post-translational carboxyl-terminal cleavage.
When the expression of streptokinase from two tac promoter-controlled expression vectors, one of these deleted a putative leader sequence of streptokinase and the other not, was compared, both normal and degraded streptokinase were detected in proteins expressed from the leader-contained vector, but only normal streptokinase was detected from the leader-deleted vector. These findings indicate that the characteristic carboxyl-terminal cleavage of streptokinase is correlated with its leader sequence and occurs during the defective secretion. The homogeneous preparation of streptokinase was facilitated by expressing from this leader-deleted vector.
Park
S K
SK
Department of Biological Science and Engineering Korea Advanced Institute of Science and Technology, Seoul.
Lee
B R
BR
Byun
S M
SM
D001483:Base Sequence; D002872:Chromosome Deletion; D004926:Escherichia coli / Q000235:genetics; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010957:Plasmids; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D013300:Streptokinase / Q000235:genetics*
D021382:Protein Sorting Signals; D013300:Streptokinase