Recombinant interleukin-1 alpha inhibits the growth of rat mesangial cells in culture.
We have investigated the effect of interleukin-1 (IL-1) on the growth of mesangial cells isolated from rat kidney. Recombinant IL-1 alpha inhibited 3H-thymidine uptake by mesangial cells in a dose-dependent manner in the presence of either 0.5% or 5% fetal bovine serum (FBS). In the presence of high concentration of FBS (10%), the effect of IL-1 was not prominent. The inhibitory effect of IL-1 on the growth of mesangial cells was also confirmed by a change in cell numbers and measurements of protein synthesis with 3H-leucine. IL-1-induced inhibition of mesangial cell growth was not affected by indomethacin. IL-1 showed no effects on intracellular Ca2+ levels in mesangial cells. These observations indicate that IL-1 inhibits the growth of mesangial cells, and thus may play a protective role for mesangial cells from abnormal proliferation in some pathophysiological states.
Ikeda
M
M
Department of Medicine, Jichi Medical School, Tochigi, Japan.
Ikeda
U
U
Oohara
T
T
Takeda
K
K
Kano
S
S
D000804:Angiotensin II; D000818:Animals; D001769:Blood; D002118:Calcium / Q000378:metabolism; D002452:Cell Count; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D005920:Glomerular Mesangium / Q000166:cytology*; D007375:Interleukin-1 / Q000502:physiology*; D007930:Leucine / Q000378:metabolism; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins; D013936:Thymidine / Q000378:metabolism
D007375:Interleukin-1; D011994:Recombinant Proteins; D000804:Angiotensin II; D004247:DNA; D007930:Leucine; D002118:Calcium; D013936:Thymidine