Structural determination of three neutral oligosaccharides in bovine (Holstein-Friesian) colostrum, including the novel trisaccharide; GalNAc alpha 1-3Gal beta 1-4Glc.
Two trisaccharides, and a pentasaccharide were obtained from bovine colostrum. Their chemical structures were determined by using methylation and 13C-NMR analyses as follows: GalNac alpha 1-3Gal beta 1-4Glc, Gal alpha-1-3Gal beta 1-4Glc, GaL beta 1-3[Gal beta 1-4GlcNAc beta 1-6]Gal beta 1-4Glc. GalNAc alpha 1-3Gal beta 1-4Glc, which was identified in this study, is a novel oligosaccharide from natural sources. Gal alpha 1-3Gal beta 1-4Glc and Gal beta 1-3[Gal beta 1-4GlcNAc beta 1-6]Gal beta 1-4Glc (lacto-N-novopentaose) have been already found in ovine colostrum, and in horse colostrum and marsupial milk, respectively.
Urashima
T
T
Department of Bioresource Chemistry, Obihiro University of Agriculture and Veterinary Medicine, Japan.
Saito
T
T
Ohmisya
K
K
Shimazaki
K
K
D000116:Acetylgalactosamine / Q000737:chemistry; D000818:Animals; D002240:Carbohydrate Sequence; D002417:Cattle; D002854:Chromatography, Paper; D003126:Colostrum / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000737:chemistry*; D010084:Oxidation-Reduction
D009844:Oligosaccharides; D000116:Acetylgalactosamine