title: The COOH-domain of the product of the Saccharomyces cerevisiae SCD25 gene elicits activation of p21-ras proteins in mammalian cells.
abstract: The evidence presented here indicates that the domain containing the COOH-terminal part of the Saccharomyces cerevisiae SCD25 gene product (C-domain), which is homologous to the COOH-terminal part of CDC25 protein, can elicit activation of mammalian ras proteins in CHO cells. Transfection of expression vectors carrying the C-domain of SCD25, but not of CDC25, promotes the GTP-bound form of ras proteins as determined by analysis of the guanine nucleotides bound to ras proteins immunoprecipitated by Y13-259 mAb, and enhances transcription of a HIV-LTR-CAT construct. This is the first demonstration of the activation of ras proteins by transfection of a single heterologous gene.
authors.affiliation: CRVA/IBV, Rhone-Poulenc Sante, Vitry sur Seine, France.
authors.forename: I
authors.initials: I
authors.lastname: Rey
authors.forename: F
authors.initials: F
authors.lastname: Schweighoffer
authors.forename: I
authors.initials: I
authors.lastname: Barlat
authors.forename: J
authors.initials: J
authors.lastname: Camonis
authors.forename: E
authors.initials: E
authors.lastname: Boy-Marcotte
authors.forename: R
authors.initials: R
authors.lastname: Guilbaud
authors.forename: M
authors.initials: M
authors.lastname: Jacquet
authors.forename: B
authors.initials: B
authors.lastname: Tocque
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D005800:Genes, Fungal; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014162:Transfection
chemical_list: D006153:Guanosine Diphosphate; D006160:Guanosine Triphosphate; D016283:Proto-Oncogene Proteins p21(ras)