The application of oligonucleotide probes to HLA class II typing of the DRB sub-region.
A plan for DRB typing at the sequence level is detailed. Only one polymerase chain amplification reaction is needed and the application of a limited number of short oligonucleotide probes allows an almost complete definition of DRB alleles. The scheme was tested on 40 homozygous cell-lines selected to cover a wide range of specificities, and 40 RFLP-typed controls. The results are presented and discussed. The simplicity and accuracy of this scheme are emphasized.
Vaughan
R W
RW
Dept. Molecular Immunogenetics, Guy's Hospital, London, U.K.
Lanchbury
J S
JS
Marsh
S G
SG
Hall
M A
MA
Bodmer
J G
JG
Welsh
K I
KI
D000483:Alleles; D001345:Autoradiography; D001483:Base Sequence; D004247:DNA / Q000032:analysis / Q000235:genetics; D006684:HLA-DR Antigens / Q000235:genetics / Q000276:immunology*; D006579:Heterozygote; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology*; D006650:Histocompatibility Testing / Q000379:methods*; D006720:Homozygote; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D000644:Quaternary Ammonium Compounds
D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D015345:Oligonucleotide Probes; D000644:Quaternary Ammonium Compounds; D004247:DNA; C027917:tetramethylammonium