title: Primary mesenchyme cell migration requires a chondroitin sulfate/dermatan sulfate proteoglycan.
abstract: Primary mesenchyme cell migration in the sea urchin embryo is inhibited by sulfate deprivation and exposure to exogenous beta-D-xylosides, two treatments known to disrupt proteoglycan synthesis. We show that in the developing sea urchin, exogenous xyloside affects the synthesis by the primary mesenchyme cells of a very large, cell surface chondroitin sulfate/dermatan sulfate proteoglycan. This proteoglycan is present in a partially purified fraction that restores migratory ability to defective cells in vitro. The integrity of this chondroitin sulfate/dermatan sulfate proteoglycan appears essential for primary mesenchyme cell migration since treatment of actively migrating cells with chondroitinase ABC reversibly inhibited their migration in vitro.
authors.affiliation: Department of Biology, University of Iowa, Iowa City 52242.
authors.forename: M C
authors.initials: MC
authors.lastname: Lane
authors.forename: M
authors.initials: M
authors.lastname: Solursh
mesh_terms: D000818:Animals; D002465:Cell Movement; D002478:Cells, Cultured; D002808:Chondroitin Lyases / Q000494:pharmacology; D011508:Chondroitin Sulfate Proteoglycans / Q000502:physiology*; D003871:Dermatan Sulfate / Q000502:physiology*; D006027:Glycosides / Q000494:pharmacology; D008648:Mesoderm / Q000166:cytology*; D012617:Sea Urchins / Q000196:embryology*; D013431:Sulfates / Q000378:metabolism; D014508:Urea / Q000494:pharmacology
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; D006027:Glycosides; D013431:Sulfates; C008646:4-nitrophenyl beta-D-xyloside; D003871:Dermatan Sulfate; D014508:Urea; D002808:Chondroitin Lyases