Phosphatidylglyceroylalkylamine, a novel phosphoglycolipid precursor in Deinococcus radiodurans.
We report here the structure of a previously uncharacterized phospholipid in the radiation-resistant bacterium Deinococcus radiodurans. This phospholipid, designated lipid 4, was shown by chemical analysis, HF hydrolysis, and nuclear magnetic resonance spectroscopy to be phosphatidylglyceroylalkylamine. Lipid 4 thus contains the unusual lipid constituents glyceric acid and alkylamines, which have previously been identified in two complex phosphoglycolipids from this organism. By [32P]phosphate pulse-chase labeling techniques, lipid 4 was shown to be the precursor of the complex phosphoglycolipids alpha-galactosyl- and alpha-N-acetylglucosaminylphosphatidylglyceroylalkylamine. While phosphatidylglyceroylalkylamine is rapidly biosynthesized from Pi, its subsequent glycosylation occurs much more slowly. Therefore, we conclude that the final glycosylation step is the rate-limiting event in the biosynthesis of the complex phosphoglycolipids alpha-galactosyl- and alpha-N-acetylglucosaminyl-phosphatidylglyceroylalkylamine.
Huang
Y
Y
Department of Microbiology and Infectious Diseases, University of Calgary, Alberta, Canada.
Anderson
R
R
D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D002250:Carbon Radioisotopes; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D006094:Gram-Positive Bacteria / Q000378:metabolism*; D007700:Kinetics; D008055:Lipids / Q000096:biosynthesis / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010710:Phosphates / Q000378:metabolism; D010715:Phosphatidylglycerols / Q000302:isolation & purification* / Q000378:metabolism; D010761:Phosphorus Radioisotopes
D000085:Acetates; D002250:Carbon Radioisotopes; D008055:Lipids; D010710:Phosphates; D010715:Phosphatidylglycerols; D010761:Phosphorus Radioisotopes; D019342:Acetic Acid