Chicken growth hormone: cDNA-synthesis and base sequence.
1. Growth hormone (GH)-cDNA was synthesized from poly A(+)-mRNA extracts of chicken pituitary glands. 2. Chicken-cDNA library was cloned into E. coli. 3. Base sequence analysis of chicken GH-cDNA revealed only 70% similarity compared with duck GH-cDNA, and 97% similarity with a previously published chicken GH-cDNA sequence. 4. Dissimilarities in base sequences are primarily observed in the 3'-non-coding region of GH-cDNAs (chicken and duck). 5. Comparisons of amino acid sequences of chicken and duck GH exhibit only three substitutions, while the amino acid sequences of GHs of chicken are identical.
Baum
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D
Abteilung Biochemie, Universität Bonn, FRG.
Graser
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G
Heib
M
M
Krampitz
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G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004372:Ducks / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D013006:Growth Hormone / Q000235:genetics*; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000032:analysis; D011061:Poly A / Q000302:isolation & purification / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid
D012333:RNA, Messenger; D011061:Poly A; D013006:Growth Hormone; D004247:DNA