The same CCAAT box-binding factor binds to the promoter of two coordinately regulated major histocompatibility complex class II genes.
Using competition mobility shift, methylation interference, and proteolytic clipping DNA binding assays, we demonstrate that the protein binding the major histocompatibility complex A beta CCAAT box is indistinguishable from the protein previously named NF-Y, which binds the major histocompatibility complex E alpha CCAAT box. Although the two CCAAT boxes share the same 10-base core sequence, termed the Y box, their flanking sequences, known to be important for binding, are very different.
Kern
M J
MJ
Department of Microbiology and Immunology, Albert B Chandler Medical Center, University of Kentucky, Lexington 40536-0084.
Woodward
J G
JG
D000818:Animals; D001483:Base Sequence; D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins / Q000378:metabolism*; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D066298:In Vitro Techniques; D008285:Major Histocompatibility Complex; D051379:Mice; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D014157:Transcription Factors / Q000378:metabolism*
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D000949:Histocompatibility Antigens Class II; D009841:Oligonucleotides; D014157:Transcription Factors