title: Solution behaviour of Chromobacter viscosum and Pseudomonas sp. lipases. No evidence of self-association.
abstract: 1. The size of two bacterial lipases was studied by SDS/PAGE, sedimentation velocity and sedimentation equilibrium to test for possible self-association behaviour. 2. Mr values of selected lipases were obtained from SDS/PAGE and sedimentation-velocity measurements, together with an absolute determination by sedimentation equilibrium 3. The Mr values obtained in a variety of aqueous solvents indicate that lipases do not self-associate in solution, suggesting the absence of surface hydrophobic patches.
authors.affiliation: Department of Applied Biochemistry and Food Science, University of Nottingham, Sutton Bonington, U.K.
authors.forename: N J
authors.initials: NJ
authors.lastname: Simpkin
authors.forename: S E
authors.initials: SE
authors.lastname: Harding
authors.forename: M P
authors.initials: MP
authors.lastname: Tombs
mesh_terms: D002861:Chromobacterium / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D008049:Lipase / Q000302:isolation & purification / Q000378:metabolism*; D046911:Macromolecular Substances; D008970:Molecular Weight; D011549:Pseudomonas / Q000201:enzymology*; D012996:Solutions
chemical_list: D046911:Macromolecular Substances; D012996:Solutions; D008049:Lipase