Radioimmunodetection of degranulated human eosinophils in mice: a potential model for imaging Hodgkin's disease and other pathologic conditions.
Various human tumors such as lymphomas and carcinomas sometimes contain extensive infiltration by degranulating eosinophils. To determine if degranulated eosinophils are suitable targets for immunolocalization, we performed in vivo distribution and imaging studies in mice, using EOS (a murine monoclonal antibody directed to human eosinophil peroxidase) labeled with indium-111. Adult mice were injected intravenously with radiolabeled EOS antibody or with similarly radiolabeled normal mouse IgG before receiving an intramuscular injection into the right thigh of homogenized human eosinophils adsorbed to latex microspheres. There was striking localization in the right thigh of the radiolabeled EOS antibody detectable by gamma imaging techniques as soon as 24 hr after injection. By contrast, there was little accumulation of radiolabeled normal IgG in the right thigh. We conclude that human eosinophil peroxidase is potentially a suitable target for radioimmunodetection and therapy of neoplasms and pathologic conditions that contain degranulating eosinophils.
Samoszuk
M K
MK
Pathology Department, University of California, Irvine 92717.
Fang
M
M
Anderson
A L
AL
D000818:Animals; D000911:Antibodies, Monoclonal; D047209:Eosinophil Peroxidase; D004804:Eosinophils / Q000201:enzymology; D005260:Female; D006689:Hodgkin Disease / Q000000981:diagnostic imaging*; D007074:Immunoglobulin G; D007205:Indium Radioisotopes; D051379:Mice; D008807:Mice, Inbred BALB C; D010544:Peroxidases / Q000276:immunology*; D011877:Radionuclide Imaging; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007205:Indium Radioisotopes; D047209:Eosinophil Peroxidase; D010544:Peroxidases