Detection by denaturing gradient gel electrophoresis of a new polymorphism in the apolipoprotein B gene.
The apolipoprotein B gene is subject to mutations that may be important in coronary heart diseases. We have used polymerase chain reaction and denaturing gradient gel electrophoresis to characterize a single nucleotide substitution in the apolipoprotein B gene. This mutation affects amino acid 4311 of the protein and converts asparagine to serine. It was found in 24% of the 81 unrelated individuals analyzed. Moreover, another mutation was detected by sequencing in a single individual.
Navajas
M
M
CNRS UPR 8402, Institut de Biologie, Montpellier, France.
Laurent
A M
AM
Moreel
J F
JF
Ragab
A
A
Cambou
J P
JP
Cuny
G
G
Cambien
F
F
Roizès
G
G
D001055:Apolipoproteins B / Q000097:blood / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D001055:Apolipoproteins B; D015345:Oligonucleotide Probes; D004247:DNA