title: Molecular cloning of an alcohol (butanol) dehydrogenase gene cluster from Clostridium acetobutylicum ATCC 824.
abstract: In Clostridium acetobutylicum, conversion of butyraldehyde to butanol is enzymatically achieved by butanol dehydrogenase (BDH). A C. acetobutylicum gene that encodes this protein was identified by using an oligonucleotide designed on the basis of the N-terminal amino acid sequence of purified C. acetobutylicum NADH-dependent BDH. Enzyme assays of cell extracts of Escherichia coli harboring the clostridial gene demonstrated 15-fold-higher NADH-dependent BDH activity than untransformed E. coli, as well as an additional NADPH-dependent BDH activity. Kinetic, sequence, and isoelectric focusing analyses suggest that the cloned clostridial DNA contains two or more distinct C. acetobutylicum enzymes with BDH activity.
authors.affiliation: Department of Biochemistry and Cell Biology, William Marsh Rice University, Houston, Texas 77251.
authors.forename: D J
authors.initials: DJ
authors.lastname: Petersen
authors.forename: R W
authors.initials: RW
authors.lastname: Welch
authors.forename: F B
authors.initials: FB
authors.lastname: Rudolph
authors.forename: G N
authors.initials: GN
authors.lastname: Bennett
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000429:Alcohol Oxidoreductases / Q000235:genetics* / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D003013:Clostridium / Q000201:enzymology / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D007527:Isoenzymes / Q000235:genetics* / Q000302:isolation & purification; D008970:Molecular Weight; D005810:Multigene Family; D011994:Recombinant Proteins / Q000302:isolation & purification; D015183:Restriction Mapping
chemical_list: D007527:Isoenzymes; D011994:Recombinant Proteins; D000429:Alcohol Oxidoreductases; C060833:butanol dehydrogenase; D000426:Alcohol Dehydrogenase