title: pHAZE: a shuttle vector system for the detection and analysis of ionizing radiation-induced mutations.
abstract: We have designed and constructed a shuttle vector system, pHAZE, that is maintained as an episome in normal human fibroblasts, has a low background mutation frequency, and is capable of detecting a spectrum of mutations, including deletions up to 8.3 kb. The efficacy of this system was demonstrated by using it to analyze mutations produced by X-rays, which induced both point mutations and large deletions in pHAZE.
authors.affiliation: Laboratory of Radiobiology and Environmental Health, University of California, San Francisco 94143-0750.
authors.forename: L H
authors.initials: LH
authors.lastname: Lutze
authors.forename: R A
authors.initials: RA
authors.lastname: Winegar
mesh_terms: D002460:Cell Line; D002872:Chromosome Deletion; D002999:Clone Cells; D004247:DNA / Q000528:radiation effects*; D005822:Genetic Vectors; D006801:Humans; D009154:Mutation; D010957:Plasmids; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004247:DNA