The effect of antirheumatic drugs on interleukin 1 (IL-1) activity and IL-1 and IL-1 inhibitor production by human monocytes.
Interleukin 1 (IL-1) is an important mediator of immune regulation, inflammation and joint destruction in arthritis. The objective of our study was to investigate the effect of a number of representative antirheumatic agents on the activity of IL-1 and the production of both IL-1 and IL-1 inhibitor by human monocytes. IL-1 activity and production were measured by its effect on the proliferation of murine thymocytes; IL-1 inhibitor production was assayed by inhibition of IL-1 mediated thymocyte proliferation. Dexamethasone (10-100 nM) inhibited IL-1 production and IL-1 mediated thymocyte proliferation. High concentrations of indomethacin, aspirin, and 12 other nonsteroidal antiinflammatory drugs (NSAID) inhibited IL-1 activity, or production or both. Methotrexate inhibited IL-1 activity without affecting IL-1 production. D-penicillamine inhibited IL-1 activity and tended to inhibit IL-1 production. Gold compounds inhibited IL-1 activity, but only auranofin inhibited IL-1 production. Acetaminophen in high concentrations (1 mg/ml) inhibited IL-1 activity and production. Colchicine inhibited IL-1 activity but not IL-1 production. No drug tested induced, by itself, the production of IL-1 inhibitor or enhanced the production of IL-1 inhibitor.
Chang
D M
DM
Department of Rheumatology/Immunology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115.
Baptiste
P
P
Schur
P H
PH
D000082:Acetaminophen / Q000494:pharmacology; D000305:Adrenal Cortex Hormones / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D018501:Antirheumatic Agents / Q000494:pharmacology; D001310:Auranofin / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D003078:Colchicine / Q000494:pharmacology; D006801:Humans; D007375:Interleukin-1 / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000494:pharmacology; D008727:Methotrexate / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D010396:Penicillamine / Q000494:pharmacology; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects; D015054:Zymosan / Q000494:pharmacology
D000305:Adrenal Cortex Hormones; D000893:Anti-Inflammatory Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D018501:Antirheumatic Agents; D007375:Interleukin-1; D000082:Acetaminophen; D001310:Auranofin; D015054:Zymosan; D010396:Penicillamine; D003078:Colchicine; D008727:Methotrexate