title: [The use of monoclonal antibodies for determining the transferrin content of the urine].
abstract: The authors used monoclonal antibodies to human transferrin for the development of a high-sensitive test system for the assessment of transferrin levels in urine with enzyme immunoassay. The conditions of the immunoassay performance were optimized. The sensitivity of the test system enabled the authors to define the levels of transferrin up to 1 ng/ml. Tests for the detection of transferrin in patients with chronic pyelonephritis and chronic renal failure were performed. The designed test system permitted transferrin to be revealed in the urine of those patients who had negative results during a radial immunodiffusion. The authors demonstrated the possibility of using the developed monoclonal antibodies in laser nephelometry for the detection of blood and urinary transferrin levels.
authors.forename: S B
authors.initials: SB
authors.lastname: Gorodetskaia
authors.forename: V A
authors.initials: VA
authors.lastname: Furalev
authors.forename: P G
authors.initials: PG
authors.lastname: Sveshnikov
authors.forename: N V
authors.initials: NV
authors.lastname: Makhlin
authors.forename: E S
authors.initials: ES
authors.lastname: Severin
mesh_terms: D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D002138:Calibration; D003456:Cryptorchidism / Q000652:urine; D006801:Humans; D007124:Immunoenzyme Techniques; D007676:Kidney Failure, Chronic / Q000652:urine; D008297:Male; D011704:Pyelonephritis / Q000652:urine; D014168:Transferrin / Q000276:immunology / Q000652:urine*
chemical_list: D000911:Antibodies, Monoclonal; D014168:Transferrin