Network antibodies identify nuclear lamin B as a physiological attachment site for peripherin intermediate filaments.
We studied the molecular associations between peripherin (a neuronal, type III intermediate filament subunit) and nuclear lamins. We show here that isolated peripherin binds selectively to mammalian lamin B under in vitro conditions. We further demonstrate that a synthetic peptide, representing the proximal part of peripherin's tail domain (P1), also associates with mammalian lamin B in a saturable, cooperative, and specific fashion. Laboratory animals immunized with P1 spontaneously develop idiotypic and anti-idiotypic antibodies recognizing peripherin and lamin B, respectively. These data provide essentially in vivo evidence that lamin B represents a constitutive nuclear "receptor" site for the tail domains of peripherin intermediate filaments.
Djabali
K
K
College de France, Biochimie Cellulaire, Paris.
Portier
M M
MM
Gros
F
F
Blobel
G
G
Georgatos
S D
SD
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000502:physiology / Q000648:ultrastructure*; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D004625:Embryo, Nonmammalian; D004912:Erythrocytes / Q000502:physiology / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D007381:Intermediate Filament Proteins / Q000235:genetics / Q000378:metabolism*; D007382:Intermediate Filaments / Q000502:physiology / Q000648:ultrastructure*; D007700:Kinetics; D034921:Lamin Type B; D034882:Lamins; D008562:Membrane Glycoproteins; D051379:Mice; D008862:Microsomes, Liver / Q000502:physiology / Q000648:ultrastructure*; D008930:Mitochondria, Liver / Q000502:physiology / Q000648:ultrastructure*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D009447:Neuroblastoma; D009474:Neurons / Q000502:physiology / Q000648:ultrastructure*; D009479:Neuropeptides / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D064531:Peripherins; D011485:Protein Binding; D051381:Rats; D015183:Restriction Mapping; D014422:Turkeys
D007381:Intermediate Filament Proteins; D034921:Lamin Type B; D034882:Lamins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D009687:Nuclear Proteins; D064531:Peripherins