title: Longitudinal histopathologic assessment of rejection after bladder-drained canine pancreas allograft transplantation.
abstract: In preparation for assessment of percutaneous biopsies in our clinical pancreas transplant program, a working knowledge of the histopathologic changes after transplantation was obtained in a longitudinal open biopsy study of 16 dogs receiving bladder-drained whole pancreas allografts. Edema, extravasation of polymorphs, and lymphocytes associated with focal parenchymal injury were early, invariable, and probably nonspecific findings. The initial feature of unmodified rejection was the appearance of capillary and small vein endothelial changes with mainly perivascular inflammatory cell infiltration. Acinar cell loss occurred early and was progressive, whereas islets and ducts were relatively preserved, indicating that acinar tissue may be more vulnerable to lytic necrosis when damaged. Functional rejection, determined by fasting urinary amylase levels, was at a stage of extensive and irreversible necrosis. Functioning grafts in immunosuppressed dogs had minor and transient endothelial changes with absence of class II antigen staining of parenchymal cells.
authors.affiliation: Pancreas Transplant Research Group, University of Sydney, Westmead Hospital, Australia.
authors.forename: R D
authors.initials: RD
authors.lastname: Allen
authors.forename: J M
authors.initials: JM
authors.lastname: Grierson
authors.forename: H
authors.initials: H
authors.lastname: Ekberg
authors.forename: W J
authors.initials: WJ
authors.lastname: Hawthorne
authors.forename: P
authors.initials: P
authors.lastname: Williamson
authors.forename: S A
authors.initials: SA
authors.lastname: Deane
authors.forename: J R
authors.initials: JR
authors.lastname: Chapman
authors.forename: G J
authors.initials: GJ
authors.lastname: Stewart
authors.forename: J M
authors.initials: JM
authors.lastname: Little
mesh_terms: D000208:Acute Disease; D000818:Animals; D001706:Biopsy; D001808:Blood Vessels / Q000473:pathology; D004285:Dogs; D004322:Drainage / Q000379:methods*; D006084:Graft Rejection; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007165:Immunosuppression Therapy; D008137:Longitudinal Studies; D010179:Pancreas / Q000098:blood supply / Q000473:pathology* / Q000503:physiopathology; D016035:Pancreas Transplantation; D010195:Pancreatitis / Q000473:pathology; D014184:Transplantation, Homologous; D001743:Urinary Bladder
chemical_list: D000949:Histocompatibility Antigens Class II