A novel MHC class I-related molecule expressed on mouse thymic stroma cells and mature lymphocytes.
A monoclonal antibody (mAb) TP-3 has been established by immunizing rats with the BALB/c mouse thymic epithelial cell line TEL-2. The TP-3 antigen is expressed on stroma cells of thymus, spleen, and lymph node in syngeneic BALB/c mice (H-2d). This antigen is also expressed at a low level on the cell surface of immature thymocytes, and at a high level on mature T and B cells. In allogeneic mice such as C57BL/6 (H-2b) or C3H (H-2k), no cells expressed the TP-3 antigen. Using H-2 congenic mice, reactivity with mAb TP-3 was found to map to a region of H-2DdLd or between Dd and Qa, suggesting that TP-3 is a major histocompatibility complex (MHC) class I antigen. However, immunoprecipitation analysis indicated that this antigen is not identical to the classical mouse class I molecules in terms of molecular size, antigenicity, and tissue distribution.
Mori
K
K
Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
Hirata
K
K
Kawabuchi
M
M
Nakashima
M
M
Watanabe
T
T
D000818:Animals; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000276:immunology; D002450:Cell Communication / Q000276:immunology; D002460:Cell Line; D004848:Epithelium / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D009928:Organ Specificity; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D015395:Histocompatibility Antigens Class I