Phytochrome-mediated activation of the gene for cytosolic glutamine-synthetase (GS1) during imbibition of photosensitive lettuce seeds.
A full-length cDNA encoding glutamine synthetase was isolated from a lambda gt11 library constructed from the poly(A)+ RNA isolated from lettuce seeds incubated under red light. The nucleotide sequence of the cDNA and the deduced sequence of amino acids showed a high degree of homology to those of the cytosol-type glutamine synthetase from other plants. Northern and dot-blot analyses of poly(A)+ RNA extracted from the seeds incubated under various light conditions showed that the activation of the gene for cytosolic glutamine-synthetase during imbibition of lettuce seeds is directly or indirectly regulated by phytochrome.
Sakamoto
A
A
Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Japan.
Takeba
G
G
Shibata
D
D
Tanaka
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D003600:Cytosol / Q000201:enzymology; D004247:DNA / Q000235:genetics; D004790:Enzyme Induction; D017343:Genes, Plant; D005974:Glutamate-Ammonia Ligase / Q000235:genetics*; D008969:Molecular Sequence Data; D010834:Phytochrome / Q000378:metabolism* / Q000528:radiation effects; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics* / Q000528:radiation effects; D011061:Poly A / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D011836:Radiation Tolerance; D012639:Seeds / Q000201:enzymology / Q000528:radiation effects; D012689:Sequence Homology, Nucleic Acid
D010940:Plant Proteins; D012333:RNA, Messenger; D010834:Phytochrome; D011061:Poly A; D004247:DNA; D005974:Glutamate-Ammonia Ligase