In vitro processing of the RNA-2-encoded polyprotein of two nepoviruses: tomato black ring virus and grapevine chrome mosaic virus.
In vitro translation of RNA-2 of each of two closely related nepoviruses, tomato black ring virus (TBRV) and grapevine chrome mosaic virus (GCMV), in a rabbit reticulocyte lysate resulted in the synthesis of single polypeptides of 150K and 146K respectively. Processing of these polyproteins occurred after the addition of translation products of homologous RNA-1. The positions of the cleavage products within the polyproteins were determined. From the N to the C terminus, Mr values for the proteins were 50K, 46K and 59K for TBRV and 44K, 46K and 56K for GCMV. TBRV RNA-1 translation products also cleaved the polyproteins encoded by GCMV RNA-2 which suggests that the cleavage sites in the two polyproteins are similar.
Demangeat
G
G
Institut de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, Strasbourg, France.
Hemmer
O
O
Fritsch
C
C
Le Gall
O
O
Candresse
T
T
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D009029:Mosaic Viruses / Q000235:genetics / Q000378:metabolism*; D010942:Plant Viruses / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics*; D011817:Rabbits; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D011506:Proteins; D012367:RNA, Viral; D014764:Viral Proteins