Macrophage colony-stimulating factor (CSF-1) gene expression in human T-lymphocyte clones.
Macrophage colony stimulating factor (CSF-1) is one of several cytokines that control the differentiation, survival, and proliferation of monocytes and macrophages. A set of 11 human T-cell clones, chosen for their phenotypic diversity, were tested for their ability to express CSF-1 mRNA. After 5 hours of stimulation with phorbol myristate acetate (PMA) + calcium ionophore (Cal), all T-cell clones expressed a major 4-kb transcript, a less abundant 2-kb transcript, and several other minor species. This pattern of expression is typical for CSF-1 mRNAs. Furthermore, of the two alloreactive T-cell clones analyzed, only one showed a definitive message for CSF-1 on specific antigenic stimulation, but with delayed kinetics and less efficiency. Both conditions of stimulation induced the release of CSF-1 protein by T cells in the culture medium. Together, these findings demonstrate for the first time that normal T cells are able to produce CSF-1, previous reports being limited to two cases of tumoral cells of the T-cell lineage.
Hallet
M M
MM
INSERM U.211, Faculté de Médecine, Laboratoire d'Hématologie, Centre Hospitalier Régional, Nantes, France.
Praloran
V
V
Vié
H
H
Peyrat
M A
MA
Wong
G
G
Witek-Giannotti
J
J
Soulillou
J P
JP
Moreau
J F
JF
D000941:Antigens / Q000276:immunology; D000001:Calcimycin / Q000494:pharmacology; D002999:Clone Cells; D015870:Gene Expression; D006084:Graft Rejection; D006086:Graft vs Host Disease / Q000473:pathology; D006801:Humans; D007519:Isoantigens / Q000276:immunology; D016173:Macrophage Colony-Stimulating Factor / Q000096:biosynthesis / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014158:Transcription, Genetic
D000941:Antigens; D007519:Isoantigens; D012333:RNA, Messenger; D000001:Calcimycin; D016173:Macrophage Colony-Stimulating Factor; D013755:Tetradecanoylphorbol Acetate