Prostaglandin E2-mediated stimulation of mucus synthesis and secretion by rhein anthrone, the active metabolite of sennosides A and B, in the mouse colon.
Rhein anthrone, the active metabolite of sennosides A and B, stimulated PGE2 release into the mouse colonic lumen. At 6.24 mg kg-1, it decreased net water and Na+ absorption significantly in the case of water, but could not reverse the net absorption in mouse ligated colon, although it enhanced net K+ secretion. Pretreatment with indomethacin diminished the effects of rhein anthrone except on K+ net secretion. Rhein anthrone or PGE2 markedly stimulated mucus secretion and synthesis in mouse ligated colon. The enhanced mucus secretion and synthesis induced by rhein anthrone were significantly suppressed by pretreatment with indomethacin. Our results have shown that the colonic secretion of water and electrolytes mediated by PGE2 is partly involved in the rhein anthrone-induced diarrhoea but that in mice, the mucoid diarrhoea induced by rhein anthrone results mainly from PGE2-mediated mucus synthesis and secretion in the colon.
Yagi
T
T
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Hyogo, Japan.
Miyawaki
Y
Y
Nishikawa
A
A
Horiyama
S
S
Yamauchi
K
K
Kuwano
S
S
D000818:Animals; D000873:Anthracenes / Q000378:metabolism / Q000494:pharmacology*; D003106:Colon / Q000378:metabolism*; D003967:Diarrhea / Q000139:chemically induced*; D015232:Dinoprostone / Q000378:metabolism*; D005260:Female; D051379:Mice; D009093:Mucus / Q000378:metabolism*; D000095322:Anthrones
D000873:Anthracenes; D015232:Dinoprostone; C055876:rhein-9-anthrone; D000095322:Anthrones