title: A mouse carcinoembryonic antigen gene family member is a calcium-dependent cell adhesion molecule.
abstract: Carcinoembryonic antigen (CEA) is a heavily glycosylated protein used clinically as a tumor marker to detect recurrences of many types of tumors. This glycoprotein belongs to the immunoglobulin superfamily and is the prototype of the large CEA family of proteins. In a concerted effort to determine the function(s) of this family, we have been investigating a similar family of proteins in the mouse. In this paper, we report the characterization of a new mouse family member named mmCGM2; this gene product is highly homologous to the human biliary glycoprotein of the CEA gene family and to a rat hepatocyte ecto-ATPase. In vitro transcription, translation, and glycosylation experiments have revealed that the mmCGM2 cDNA encodes a glycoprotein of 42 kDA with a putative extracellular N-terminal domain and a C2-set type immunoglobulin domain. We have used this cDNA as a probe to detect many different transcripts (1.5-4.6 kilobases) in mouse adult tissues, some of which are specific to particular tissues, while others are expressed ubiquitously. After transfection of a plasmid bearing the mmCGM2 cDNA into mouse fibroblasts known to lack CEA-related gene expression, transfectant cell clones were chosen and used to investigate the adhesion properties conferred onto the cells. Cells expressing the mmCGM2 cDNA in a sense orientation aggregated in a calcium- and temperature-dependent fashion. Together with human biliary glycoprotein, the mmCGM2 gene product is the first member of the immunoglobulin superfamily to exhibit calcium-dependent adhesion. The constant tissue reorganization necessary to the differentiation of precise structures in tissues which express these gene family members (colon, liver, and uterus) implies the necessity of a variety of specific cell-cell contacts which could utilize the cell adhesion properties that we have demonstrated.
authors.affiliation: McGill Cancer Centre, Montreal, Quebec, Canada.
authors.forename: C
authors.initials: C
authors.lastname: Turbide
authors.forename: M
authors.initials: M
authors.lastname: Rojas
authors.forename: C P
authors.initials: CP
authors.lastname: Stanners
authors.forename: N
authors.initials: N
authors.lastname: Beauchemin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002272:Carcinoembryonic Antigen / Q000235:genetics*; D015815:Cell Adhesion Molecules / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D005260:Female; D006801:Humans; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D005810:Multigene Family; D010957:Plasmids; D014176:Protein Biosynthesis; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D002272:Carcinoembryonic Antigen; D015815:Cell Adhesion Molecules