Cloning and structure of cDNA encoding alpha-latrotoxin from black widow spider venom.
cDNA encoding the putative alpha-latrotoxin precursor was isolated from spider venom glands cDNA library and sequenced. The cDNA contained the 4203 base-pair open reading frame corresponding to the 156,855-Da protein composed of 1401 amino acids. Computer analysis of the deduced primary structure revealed the presence of various internal imperfect repeats mainly in its central and C-terminal regions.
Kiyatkin
N I
NI
Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow.
Dulubova
I E
IE
Chekhovskaya
I A
IA
Grishin
E V
EV
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015723:Gene Library; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics; D011061:Poly A / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D013111:Spider Venoms / Q000737:chemistry / Q000235:genetics*
D010446:Peptide Fragments; D012333:RNA, Messenger; D013111:Spider Venoms; D011061:Poly A; C020763:alpha-latrotoxin