Stimulation of macrophage growth and multinucleated cell formation in rat bone marrow cultures by insulin-like growth factor I.
In this study the effects of rhIGF-I on macrophage differentiation and growth have been studied using liquid suspension cultures of rat bone marrow cells. IGF-I stimulated macrophage growth in a dose-dependent manner, a maximum response was found at a concentration of 20 ng/ml. IGF-I effects could be ascribed to stimulation of both postmitotic and proliferating cells. A remarkable finding was that IGF-I induced formation of multinucleated cells (MNC). The MNC resembled macrophage-like cells (AcP, NSE positive). A monoclonal antibody to rhIGF-I significantly inhibited IGF-stimulated macrophage growth and MNC formation. A specific antibody to mouse CSF-1 reduced IGF-stimulated macrophage growth in mouse bone marrow cultures indicating that IGF-I effects could, at least in part, be ascribed to endogenous production of CSF-1. These findings indicate that IGF-I in concert with locally induced CSF-1 can influence the differentiation and growth of bone marrow-derived macrophages.
Scheven
B A
BA
Rowett Research Institute, Bucksburn, Aberdeen, UK.
Hamilton
N J
NJ
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007700:Kinetics; D016173:Macrophage Colony-Stimulating Factor / Q000276:immunology / Q000502:physiology; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology
D000906:Antibodies; D000911:Antibodies, Monoclonal; D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I; D016173:Macrophage Colony-Stimulating Factor