Genetically engineered S and F1C fimbriae differ in their contribution to adherence of Escherichia coli to cultured renal tubular cells.
Escherichia coli K-12 strains producing S-fimbrial adhesins, F1C fimbriae, and mutagenized fimbriae were tested in a binding assay with a renal tubular cell line. S-fimbrial adhesins and F1C fimbriae mediated binding to tubular cells. The SfaA, SfaG, and SfaS subunits of S fimbriae contributed to attachment. Site-specific mutations in the sfaS gene reduced binding. The inhibition profile of F1C fimbriae resembled that of S fimbriae.
Marre
R
R
Institut für Medizinische Mikrobiologie, Universität Lübeck, Federal Republic of Germany.
Kreft
B
B
Hacker
J
J
D018830:Adhesins, Escherichia coli; D000818:Animals; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D002460:Cell Line; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D010861:Fimbriae, Bacterial / Q000276:immunology / Q000502:physiology*; D005798:Genes, Bacterial; D007684:Kidney Tubules / Q000382:microbiology*; D011993:Recombinant Fusion Proteins; D013552:Swine
D018830:Adhesins, Escherichia coli; D001425:Bacterial Outer Membrane Proteins; D011993:Recombinant Fusion Proteins