Erythrocytes as carriers for L-asparaginase. Methodological and mouse in-vivo studies.
L-Asparaginase has been encapsulated in Swiss mouse or human erythrocytes by hypotonic haemolysis followed by isotonic resealing and reannealing. The details of incorporation and properties of carrier erythrocytes are presented. When L-asparaginase loaded into 51Cr-labelled erythrocytes, was infused intravenously, the same half-life was found for asparaginase and 51Cr. In addition, L-asparaginase loaded into erythrocytes was much more effective in eliminating plasma asparagine compared with the same dose of free L-asparaginase injected in solution, during a sustained period (14 days).
Kravtzoff
R
R
Centre Régional de Transfusion Sanguine INSERM U 3162, Tours, France.
Ropars
C
C
Laguerre
M
M
Muh
J P
JP
Chassaigne
M
M
D000818:Animals; D001215:Asparaginase / Q000008:administration & dosage* / Q000378:metabolism / Q000493:pharmacokinetics; D001216:Asparagine / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D002860:Chromium Radioisotopes; D004337:Drug Carriers; D004912:Erythrocytes / Q000378:metabolism; D006400:Hematocrit; D006801:Humans; D007262:Infusions, Intravenous; D007457:Iodine Radioisotopes; D051379:Mice
D002860:Chromium Radioisotopes; D004337:Drug Carriers; D007457:Iodine Radioisotopes; D001216:Asparagine; D001215:Asparaginase