The estrogen-regulated destabilization of Xenopus albumin mRNA is independent of translation.
Protein synthesis inhibitors have been shown to increase the stability of a number of labile mRNAs. In Xenopus laevis serum albumin mRNA is destabilized in the liver cell cytoplasm following estrogen administration. The present study examined the effect of translation inhibitors on this process. The initiation inhibitor 2-(4-methyl-2,6-dinitroanilino)-N-methylpropionamide causes accumulation of albumin mRNA in 20-80S mRNP particles whereas the elongation inhibitor cycloheximide causes albumin mRNA to accumulate in polysomes. Neither inhibitor blocked the disappearance of albumin mRNA from liver cell cytoplasm when added with estradiol to the medium of liver explant cultures. We conclude that unlike a number of labile mRNAs the instability of Xenopus albumin mRNA following estradiol is independent of translation.
Moskaitis
J E
JE
Department of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799.
Buzek
S W
SW
Pastori
R L
RL
Schoenberg
D R
DR
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009924:Organ Culture Techniques; D011132:Polyribosomes / Q000378:metabolism; D011422:Propionates / Q000494:pharmacology; D014176:Protein Biosynthesis; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics*; D012261:Ribonucleoproteins / Q000378:metabolism; D012709:Serum Albumin / Q000235:genetics*; D014982:Xenopus laevis
D011422:Propionates; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D012709:Serum Albumin; C026452:messenger ribonucleoprotein; C002167:2-(4-methyl-2,6-dinitroanilino)-N-methylpropionamide; D004958:Estradiol; D003513:Cycloheximide