title: Nonisotopic in situ hybridization as a method for nondisjunction studies in human spermatozoa.
abstract: Human spermatozoa were studied with a nonradioactive in situ hybridization method. Using a chemically modified DNA probe and immunocytochemical reactions for visualization, it was possible to obtain hybridization signals in 31 of 32 semen samples. Positive hybridization reactions, depending on cell accessibility, varied from 40% to over 90% for the different samples. Using a chromosome 1-specific DNA probe, disomy for this chromosome was found in 0.67% of all accessible sperm cells.
authors.affiliation: Department of Genetics/Molecular Cell Biology, University of Limburg, Maastricht, The Netherlands.
authors.forename: E
authors.initials: E
authors.lastname: Coonen
authors.forename: M H
authors.initials: MH
authors.lastname: Pieters
authors.forename: J C
authors.initials: JC
authors.lastname: Dumoulin
authors.forename: H
authors.initials: H
authors.lastname: Meyer
authors.forename: J L
authors.initials: JL
authors.lastname: Evers
authors.forename: F C
authors.initials: FC
authors.lastname: Ramaekers
authors.forename: J P
authors.initials: JP
authors.lastname: Geraedts
mesh_terms: D000328:Adult; D000782:Aneuploidy; D004229:Dithiothreitol / Q000494:pharmacology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009630:Nondisjunction, Genetic; D009693:Nucleic Acid Hybridization; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D004229:Dithiothreitol