Immunohistochemical localization of testicular sulphydryloxidase during sexual maturation of the Djungarian hamster (Phodopus sungorus).
Immunohistochemical localization of sulphydryloxidase was examined in the testis of the Djungarian hamster from Day 0 to Day 31 of post-natal development. The sulphydryloxidase antibody labelled prespermatogonia and the first population of spermatogonia type A within the seminiferous epithelium. Additionally, Sertoli cells exhibited immunoreactivity from Day 2 to Day 11 after birth. From Day 11 onwards, sulphydryloxidase immunoreactivity was found in germ cells after the initiation spermatogenesis from pachytene primary spermatocytes, showing the highest intensity in mid-pachytene spermatocytes. The pattern of sulphydryloxidase expression during spermatogenesis was identical to that found in adult animals. It is concluded that sulphydryloxidase immunoreactivity not only serves as a marker for early stages of spermatogenesis, especially pachytene spermatocytes, confirming earlier reports, but also for spermatogonial precursors.
Bergmann
M
M
Institute of Anatomy, University of Münster, FRG.
Oehmen
F
F
Kumari
M
M
Aumüller
G
G
Seitz
J
J
D000818:Animals; D006224:Cricetinae; D007150:Immunohistochemistry; D008297:Male; D010088:Oxidoreductases / Q000032:analysis*; D012708:Sertoli Cells / Q000201:enzymology; D012741:Sexual Maturation / Q000502:physiology*; D013090:Spermatocytes / Q000201:enzymology; D013093:Spermatogonia / Q000201:enzymology; D013737:Testis / Q000166:cytology / Q000201:enzymology*; D050862:Oxidoreductases Acting on Sulfur Group Donors
D010088:Oxidoreductases; D050862:Oxidoreductases Acting on Sulfur Group Donors