title: The N-terminal sequence of the major erythropoietic factor of an anephric patient is identical to insulin-like growth factor I.
abstract: The erythropoietic factors present in an anephric patient with nearly normal hematocrit were isolated from plasma by reversed-phase and gel permeation HPLC. The most active fraction was purified and the analysis of its N-terminal sequence was identical to the published sequence of the human insulin-like growth factor I (IGF I). Recombinant human IGF I had identical elution positions as the isolated erythropoietic factor on reversed-phase HPLC and the same molecular weight on gel permeation HPLC. Furthermore, hrIGF I stimulated erythroid colony formation in human bone marrow cultures as was previously shown for the isolated human erythropoietic factor. These results suggest that IGF I may replace erythropoietin as a stimulator of erythropoiesis in some patients with anemia and renal failure.
authors.affiliation: Endocrine Laboratory, Royal Victoria Hospital, Montreal, Canada.
authors.forename: L F
authors.initials: LF
authors.lastname: Congote
authors.forename: A
authors.initials: A
authors.lastname: Brox
authors.forename: F K
authors.initials: FK
authors.lastname: Lin
authors.forename: H S
authors.initials: HS
authors.lastname: Lu
authors.forename: A A
authors.initials: AA
authors.lastname: Fauser
mesh_terms: D000595:Amino Acid Sequence; D004921:Erythropoietin / Q000737:chemistry*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000737:chemistry*; D007668:Kidney / Q000002:abnormalities*; D007676:Kidney Failure, Chronic / Q000378:metabolism
chemical_list: D004921:Erythropoietin; D007334:Insulin-Like Growth Factor I