title: Organization and expression of two tandemly oriented genes encoding ribulosebisphosphate carboxylase/oxygenase activase in barley.
abstract: We have isolated and structurally characterized genomic DNA and cDNA sequences encoding ribulose-1,5-bisphosphate carboxylase/oxygenase (Rbu-P2 carboxylase) activase from barley (Hordeum vulgare L.). Three Rbu-P2 carboxylase activase (Rca) polypeptides are encoded in the barley genome by two closely linked, tandemly oriented nuclear genes (RcaA and RcaB); cDNAs encoding each of the three Rbu-P2 carboxylase activase polypeptides were isolated from cDNA libraries of barley leaf mRNA. RcaA produces two mRNAs, which encode polypeptides of 42 and 46 kDa, by an alternative splicing mechanism identical to that previously reported for spinach and Arabidopsis Rca genes (Werneke, J.M., Chatfield, J.M., and Ogren, W. L. (1989) Plant Cell 1, 815-825). RcaB is transcribed to produce a single mRNA, which encodes a mature peptide of 42 kDa. Genomic Southern blots indicate that RcaA and RcaB represent the entire Rbu-P2 carboxylase activase gene family in barley. The genes share 80% nucleotide sequence identity, and the 42-kDa polypeptides encoded by RcaA and RcaB share 87% amino acid sequence identity. Coding regions of the two barley Rca genes are separated by 1 kilobase pair of flanking DNA. DNA sequence motifs similar to those thought to control light-regulated gene expression in other nuclear-encoded plastid polypeptide genes are found at the 5' end of both barley Rca genes. Probes specific to three mRNAs were used to determine the relative contribution each species makes to the total Rca mRNA pool.
authors.affiliation: Department of Plant Biology, University of Illinois, Urbana 61801.
authors.forename: S J
authors.initials: SJ
authors.lastname: Rundle
authors.forename: R E
authors.initials: RE
authors.lastname: Zielinski
mesh_terms: D000595:Amino Acid Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D004247:DNA / Q000032:analysis; D004789:Enzyme Activation; D015971:Gene Expression Regulation, Enzymologic; D001467:Hordeum / Q000201:enzymology*; D008969:Molecular Sequence Data; D010940:Plant Proteins; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; C054765:rca protein, plant; D004247:DNA; D012273:Ribulose-Bisphosphate Carboxylase