The effects of beta agonists on muscle cells in culture.
Increases in protein synthesis of 12% were found with two myogenic cell lines (L6 and G8-1) on treatment for 6 hr with the beta-adrenergic agonist cimaterol. In L6 cells, propranolol blocked the effect. Protein breakdown measured over 18-24 hr was unchanged. The Kd for cimaterol binding to the L6 beta-receptor was 26 nM which was compatible with its EC50 for the stimulation of protein synthesis (approx 5 nM). Evidence provided with muscle cell lines indicates a direct effect of cimaterol on protein synthesis, which may contribute to muscle accretion in cimaterol-fed animals.
Harper
J M
JM
Department of Applied Biochemistry and Food Science, University of Nottingham, Faculty of Agricultural Science, Loughborough, Leics.
Mackinson
I
I
Buttery
P J
PJ
D000318:Adrenergic beta-Agonists / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*; D000420:Albuterol / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D002976:Clenbuterol / Q000378:metabolism / Q000494:pharmacology; D004837:Epinephrine / Q000378:metabolism / Q000494:pharmacology; D004983:Ethanolamines / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*; D008024:Ligands; D009132:Muscles / Q000166:cytology / Q000187:drug effects*; D011433:Propranolol / Q000378:metabolism / Q000494:pharmacology; D011943:Receptors, Adrenergic, beta / Q000378:metabolism
D000318:Adrenergic beta-Agonists; D004983:Ethanolamines; D008024:Ligands; D011943:Receptors, Adrenergic, beta; D011433:Propranolol; D000420:Albuterol; D002976:Clenbuterol; D004837:Epinephrine; C047411:cimaterol