title: Molecular cloning, sequence, and tissue distribution of the human ubiquitin-activating enzyme E1.
abstract: The ubiquitin-activating enzyme E1 catalyzes the first step in ubiquitin conjugation. We have cloned and sequenced the cDNA for human E1. This clone predicts a protein of 110,450 Da. Cys-194 lies within a region of identity to active-site Cys-88 of the ubiquitin carrier protein E2, suggesting a potential role for this region in enzymatic function of this protein. In addition, Cys-454 lies within a region of identity to the thiol ester consensus sequence of several proteins involved in thioester formation. Tissue distribution reveals a single 3.5-kilobase E1 message ubiquitous among tissues and cell lines.
authors.affiliation: Department of Cell Biology, Washington University School of Medicine, St. Louis, MO.
authors.forename: P M
authors.initials: PM
authors.lastname: Handley
authors.forename: M
authors.initials: M
authors.lastname: Mueckler
authors.forename: N R
authors.initials: NR
authors.lastname: Siegel
authors.forename: A
authors.initials: A
authors.lastname: Ciechanover
authors.forename: A L
authors.initials: AL
authors.lastname: Schwartz
mesh_terms: D000595:Amino Acid Sequence; D000906:Antibodies / Q000302:isolation & purification; D001483:Base Sequence; D003001:Cloning, Molecular; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D015723:Gene Library; D006801:Humans; D008025:Ligases / Q000097:blood / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D014176:Protein Biosynthesis; D014158:Transcription, Genetic; D044764:Ubiquitin-Activating Enzymes; D044767:Ubiquitin-Protein Ligases
chemical_list: D000906:Antibodies; D044767:Ubiquitin-Protein Ligases; D008025:Ligases; D044764:Ubiquitin-Activating Enzymes