DNA sequences 3' of the Ig H chain cluster rearrange in mouse B cell lines.
A mouse myeloma cell line MPC11 (IgG2b, kappa) and variants derived from it have been used to study DNA rearrangements that occur at the Ig H chain locus. One variant, F5.5, has acquired both VH gene and C epsilon gene rearrangements. Through genomic Southern blot analysis initially directed to mapping the C epsilon gene rearrangement, we observed that the VH region rearrangement was linked, through an inversion event, to sequences that originate 3' of the CH cluster, i.e., 3' of the C alpha gene. Subsequent studies have shown that DNA rearrangements within the region 3' of the C alpha gene are detected in several other mouse myeloma and hybridoma cell lines and are not associated with the expression of specific isotypes.
Calvo
C F
CF
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461.
Giannini
S L
SL
Martinez
N
N
Birshtein
B K
BK
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D002874:Chromosome Mapping; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain / Q000235:genetics*; D015698:Genomic Library; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma; D014407:Tumor Cells, Cultured