Lack of feedback inhibition of V kappa gene rearrangement by productively rearranged alleles.
Circular DNAs excised by immunoglobulin kappa chain gene rearrangements were cloned and characterized. 16 of 17 clones examined were double recombination products containing a V kappa-J kappa rearrangement (coding joint) as well as the reciprocal element (signal joint) of another V kappa-J kappa rearrangement. These products suggested multiple recombination, primary inversion, and secondary excision. In primary events, 5 of 16 translational reading frames were in-phase. Thus, V kappa gene rearrangement may not be inhibited by the presence of a productively rearranged allele. An unusually large trinucleotide (P) insertion forming a palindrome of 12 nucleotides was also observed in one of the coding joints.
Harada
K
K
Department of Biophysics, Faculty of Science, Kyoto University, Japan.
Yamagishi
H
H
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004270:DNA, Circular / Q000235:genetics; D005246:Feedback; D015322:Gene Rearrangement, B-Lymphocyte / Q000235:genetics*; D007133:Immunoglobulin Joining Region / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis
D004270:DNA, Circular; D007133:Immunoglobulin Joining Region; D007135:Immunoglobulin Variable Region