Increase in the level of P-glycoprotein mRNA expression in multidrug-resistant K562 cell lines treated with sodium butyrate is not accompanied with erythroid differentiation.
We examined the effect of hemin, sodium butyrate and mitomycin C on levels of P-glycoprotein mRNA in human myelogenous K562 cells by northern blot analysis. After treatment with sodium butyrate a dose-dependent increase of P-glycoprotein mRNA expression was observed in the adriamycin-resistant K562 and vincristine-resistant K562 lines. With 10 mM sodium butyrate, the level of P-glycoprotein mRNA reached 20 times that of control adriamycin-resistant K562 and with 30 mM sodium butyrate, it exceeded 5 times that of control vincristine-resistant K562. In contrast, hemin and mitomycin C had almost no effect on P-glycoprotein mRNA. In this experiment, since expression of P-glycoprotein mRNA was not necessarily accompanied with induction of erythroid differentiation, the increased amount of P-glycoprotein mRNA is unlikely to be a result of differentiation.
Shibata
H
H
Department of Clinical Cancer Chemotherapy, Tohoku University, Sendai.
Kanamaru
R
R
Sato
T
T
Ishioka
C
C
Konishi
Y
Y
Ishikawa
A
A
Wakui
A
A
Tsuruo
T
T
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D015152:Blotting, Northern; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line / Q000187:drug effects / Q000378:metabolism; D004317:Doxorubicin / Q000494:pharmacology; D004351:Drug Resistance / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D006427:Hemin / Q000494:pharmacology; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis*; D014750:Vincristine / Q000494:pharmacology
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D002087:Butyrates; D008562:Membrane Glycoproteins; D008937:Mitomycins; D012333:RNA, Messenger; D020148:Butyric Acid; D016685:Mitomycin; D014750:Vincristine; D006427:Hemin; D004317:Doxorubicin