title: Expression of the insulin-like and platelet-derived growth factor genes in human uterine tissues.
abstract: The human uterus repeatedly exhibits cyclic biochemical and cytological changes during the reproductive period of life. These changes are the result of a well-characterized endocrine network involving the hypothalamus, pituitary, and ovary. The exact nature of the mechanism(s) by which the sex steroids act on the uterus remains to be elucidated. Possible local mediators of hormonal action on the uterus include polypeptide growth factors. Using the method of RNA transfer blot hybridization, we have analyzed tissue samples from the cycling human endometrium and tissue samples of human myometrium and myometrial benign tumor (leiomyoma) for the presence of platelet-derived growth factor (PDGF) and insulin-like growth factor (IGF) RNA. All the uterine tissues examined possessed RNA for PDGF-B chain and IGF-I and -II. Two transcripts were observed for PDGF-B chain, four were observed for IGF-I, and eight were observed for IGF-II. Overall, the relative abundance of PDGF-B chain RNA was consistent in all of the uterine tissues examined. In contrast, IGF RNA relative abundance varied. IGF-I RNA was highest in late proliferative stage endometrium, and IGF-II RNA was highest in early proliferative stage endometrium. Both IGF-I and IGF-II RNAs were greater in amount of leiomyoma than in myometrium. The increased IGF-I RNA in late proliferative-stage human endometrium correlates with the known elevation of estradiol secretion by the ovary and the increased concentration of uterine estradiol receptors during this stage of the menstrual cycle.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Reproductive Biology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.
authors.forename: K D
authors.initials: KD
authors.lastname: Boehm
authors.forename: M
authors.initials: M
authors.lastname: Daimon
authors.forename: I G
authors.initials: IG
authors.lastname: Gorodeski
authors.forename: L A
authors.initials: LA
authors.lastname: Sheean
authors.forename: W H
authors.initials: WH
authors.lastname: Utian
authors.forename: J
authors.initials: J
authors.lastname: Ilan
mesh_terms: D004717:Endometrium / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D007889:Leiomyoma / Q000378:metabolism; D008597:Menstrual Cycle / Q000502:physiology; D009215:Myometrium / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D011061:Poly A / Q000096:biosynthesis; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger / Q000096:biosynthesis; D014594:Uterine Neoplasms / Q000378:metabolism
chemical_list: D010982:Platelet-Derived Growth Factor; D011518:Proto-Oncogene Proteins; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger; D011061:Poly A; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II