Chronopharmacokinetics of 5-methoxypsoralen.
Diurnal variations in drug pharmacokinetics are a well known phenomenon. Chronopharmacology studies now appear to be attracting increasing interest with a view to establishing an optimum therapeutic prescription. In order to determine possible chronobiological variations of 5-methoxypsoralen (5-MOP) pharmacokinetic, 5-MOP blood concentrations were quantified in 8 healthy subjects after drug ingestion at different times during the day. Stolk's High Performance Liquid Chromatography technique was used to assess the 5-MOP serum concentrations. Each subject underwent three pharmacokinetic studies after oral ingestion of 5-MOP (1.2 mg/kg), in conjunction with a standardized low-lipid meal. The first pharmacokinetic study was started in the morning, the second in the afternoon and the third in the evening. Drug intake was at intervals of 2 days, to avoid drug accumulation. The results showed that the evening intake of the drug induced a higher 5-MOP maximum concentration and a higher 'area under curve' than morning or afternoon ingestion. This study suggests an optimized PUVA therapy, when performed in the evening.
Treffel
P
P
Faculté de Médecine et Pharmacie, Besancon, France.
Renaud
A
A
Humbert
P
P
Makki
S
S
Faivre
B
B
Agache
P G
PG
D000078223:5-Methoxypsoralen; D000328:Adult; D002851:Chromatography, High Pressure Liquid; D002940:Circadian Rhythm; D005260:Female; D006801:Humans; D008297:Male; D008730:Methoxsalen / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics*
D000078223:5-Methoxypsoralen; D008730:Methoxsalen