Recommended protocols based on a survey of current practice in genotoxicity testing laboratories, IV. Chromosome aberration and sister-chromatid exchange in Chinese hamster ovary, V79 Chinese hamster lung and human lymphocyte cultures.
A recommended protocol has been developed for chromosomal aberration and sister-chromatid exchange assays in CHO, V79 and human lymphocyte cultures. The protocol was based on the responses to a detailed questionnaire completed by North-American and European governmental, university, and contract laboratories using these tests. This report identifies those modifications to previously described methods that are used on a regular basis and clarifies confusing or inconsistent practices. These protocols can be modified for use in other types of cells.
Swierenga
S H
SH
Bureau of Drug Research, Health Protection Branch, Health and Welfare Canada, Ottawa, Ont.
Heddle
J A
JA
Sigal
E A
EA
Gilman
J P
JP
Brillinger
R L
RL
Douglas
G R
GR
Nestmann
E R
ER
D000818:Animals; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D006224:Cricetinae; D005260:Female; D066298:In Vitro Techniques; D008168:Lung / Q000166:cytology; D008214:Lymphocytes / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D009152:Mutagenicity Tests / Q000379:methods* / Q000592:standards; D010053:Ovary / Q000166:cytology; D012107:Research Design; D012854:Sister Chromatid Exchange