Activation of lymphocytes after platelet allotransfusion possessing only class I MHC product.
After platelet allotransfusion, we found a characteristic increase in the expression of interleukin-2 receptor, dipeptydilpeptidase IV (CD26), activation-inducer molecule (AIM, CD69) and transferrin receptors (CD71) on day 3 indicating that important functional molecules expressed on the activation of lymphocytes by allogeneic platelets. At the same time, no consistent increase of other activation molecules such as Ki-l (CD30), intercellular adhesion molecule (ICAM-1, CD54) and Ki-24 (CDw70) antigen expression was detected, probably as a result of the selective activation of some lymphocyte subsets. In order to obtain further evidence for the in vivo activation triggered by allogeneic platelets, subsequent step of T cell activation towards differentiation was investigated with monoclonal antibodies to leucocyte common antigens. A sharp expression of the UCHL1, coupled with a decrease of the CD45R molecule was detected on day 7 or 14, suggesting a T cell priming.
Pócsik
E
E
Department of Cellular Immunology, National Institute of Haematology and Blood Transfusion, Budapest, Hungary.
Mihalik
R
R
Gyódi
E
E
Réti
M
M
Pálóczi
K
K
Petrányi
G G
GG
Benczúr
M
M
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000096:biosynthesis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000096:biosynthesis; D001792:Blood Platelets / Q000276:immunology*; D001803:Blood Transfusion; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000096:biosynthesis; D015395:Histocompatibility Antigens Class I / Q000008:administration & dosage / Q000276:immunology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D037181:Lectins, C-Type; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D017713:Platelet Transfusion; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000276:immunology; D000099192:CD69 Antigens
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D015815:Cell Adhesion Molecules; D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D015395:Histocompatibility Antigens Class I; D018799:Intercellular Adhesion Molecule-1; D037181:Lectins, C-Type; D015375:Receptors, Interleukin-2; D000099192:CD69 Antigens