title: Activation of protein kinase C is essential for sustained insulin secretion in response to cholinergic stimulation.
abstract: Insulin secretion from isolated rat islets of Langerhans is enhanced by cholinergic agonists, such as carbachol (CCh), in the presence of a stimulatory concentration of glucose. Depletion of islet protein kinase C activity by prolonged exposure to a tumour-promoting phorbol ester did not prevent the initial secretory response to CCh, but markedly reduced the duration of CCh-induced elevated secretory rates. These results suggest that the major action of PKC is in maintaining rather than initiating the insulin secretory response to cholinergic agonists.
authors.affiliation: Biomedical Sciences Division, King's College London, Kensington, U.K.
authors.forename: S J
authors.initials: SJ
authors.lastname: Persaud
authors.forename: P M
authors.initials: PM
authors.lastname: Jones
authors.forename: S L
authors.initials: SL
authors.lastname: Howell
mesh_terms: D000818:Animals; D002217:Carbachol / Q000494:pharmacology*; D015536:Down-Regulation; D004789:Enzyme Activation / Q000502:physiology; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D011493:Protein Kinase C / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D007328:Insulin; D002217:Carbachol; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate