Restriction fragment length polymorphisms in the major histocompatibility complex of the non-obese diabetic mouse.
The inbred non-obese diabetic (NOD) mouse is a spontaneous model for insulin-dependent diabetes mellitus (IDDM). As in man and BB rats, IDDM in the NOD mouse has an autoimmune aetiology. The disease is controlled by several genes, one of which, Idd-1, has been mapped to the major histocompatibility complex (MHC) on chromosome 17. However, Idd-1 has not yet been identified. To facilitate the identification of Idd-1 we have further analysed the MHC region for restriction fragment length polymorphisms and we find that the NOD mouse has a distinct haplotype: H-2K1nod Kd A beta nod A alpha d E beta nod TNF-alpha beta. In addition, the NOD mouse shows some similarities with the H-2b haplotype in the Q region, in that either the Q7 or the Q9 gene seems to be like that in the b-haplotype and that the Qa2 antigen is expressed, while other parts of this region are distinct from the b- as well as the d- haplotype. In contrast, the sister strain, the non-obese normal (NON) mouse, derived from the same cataract-prone line of mice as the NOD mouse, has an MHC Class I region indistinguishable from the b-haplotype, but the MHC Class II region is distinct from the NOD mouse as well as the b-, d- and k-haplotype.
Lund
T
T
Department of Immunology, University College and Middlesex School of Medicine, London, UK.
Simpson
E
E
Cooke
A
A
D000818:Animals; D001327:Autoimmune Diseases / Q000235:genetics; D015139:Blotting, Southern; D004247:DNA / Q000302:isolation & purification; D015342:DNA Probes; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D015870:Gene Expression; D006239:Haplotypes; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D008099:Liver; D008285:Major Histocompatibility Complex / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains; D012150:Polymorphism, Restriction Fragment Length; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D015342:DNA Probes; D000949:Histocompatibility Antigens Class II; D014409:Tumor Necrosis Factor-alpha; D004247:DNA