Expression of c-erbB-2 gene product in urinary bladder cancer.
Expression of the c-erbB-2 gene product and the epidermal growth factor receptor (EGF-R) was investigated in 54 cases of human bladder cancer immunohistologically and by Western blot analysis. For detection of the c-erbB-2 product, two specific antibodies, a rabbit polyclonal antibody directed to the intracellular domain and a murine monoclonal antibody recognizing an epitope in the extracellular domain, were used. Seventeen cases of bladder cancer were stained by the anti-c-erbB-2 polyclonal antibody, while 20 cases were stained by the monoclonal antibody, with good correlation on both stainings (p less than 0.01). There were four c-erbB-2 positive cases in 26 G1 tumors, four in 15 G2 tumors, and nine in 13 G3 tumors. There were also eight erbB-2 positive cases in nine muscle-invasive tumors, nine of 45 superficial tumors, four of five with lymph node metastasis, and seven of 14 without metastasis, as revealed by staining with the polyclonal antibody. Thus, the c-erbB-2 gene product was more frequently expressed in high grade tumors (p less than 0.01), in high stage tumors (p less than 0.01), and nodal metastatic tumors (N.S. by Chi-square test). Twenty-two of the 54 tumors were stained by an anti-EGF-R monoclonal antibody, 528 IgG. The expression of EGF-R was independent of histological grading, tumor stage, and nodal status, and no correlation was observed between expression of the c-erbB-2 product and EGF-R. The c-erbB-2 product may be applicable as a tumor marker for evaluation of malignant potential, invasiveness, and probably metastatic potential of human bladder cancer.
Moriyama
M
M
Department of Pathology and Oncology, Institute of Medical Science, University of Tokyo, Japan.
Akiyama
T
T
Yamamoto
T
T
Kawamoto
T
T
Kato
T
T
Sato
K
K
Watanuki
T
T
Hikage
T
T
Katsuta
N
N
Mori
S
S
D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002295:Carcinoma, Transitional Cell / Q000737:chemistry / Q000235:genetics*; D066246:ErbB Receptors / Q000235:genetics*; D006801:Humans; D011506:Proteins / Q000235:genetics; D011519:Proto-Oncogenes; D001749:Urinary Bladder Neoplasms / Q000737:chemistry / Q000235:genetics*
D000911:Antibodies, Monoclonal; D011506:Proteins; D066246:ErbB Receptors