A mouse endothelial cell-specific monoclonal antibody: its reactivity with LTMC endothelium.
The binding of a marrow cell-related monoclonal antibody (H513E3 MAb) has been investigated in long-term marrow cultures (LTMC) and in vivo. Immunogold labeling and electron microscopy revealed that this antibody labeled an endothelial-like cell. Cross-reaction of anti-human Factor VIII confirmed endothelial specificity of the H513E3 MAb. In addition, vessel endothelium (vena cava, aorta, and marrow) exhibited binding of the antibody. This antibody provides a unique tool to study the cellular architecture of LTMC and implicates endothelium as an important component of LTMC. The function of the endothelial cell-specific surface antigen is unknown, although preferential labeling of the upper surface of endothelial cells suggests that it may play a role in cell communication, particularly with the floating population. The H513E3 MAb reacts with an external membrane antigen, a property that makes this antibody particularly useful for fluorescences-activated sorting of endothelial cells.
Hasthorpe
S
S
Cell Biology Unit, Peter MacCallum Cancer Institute, Melbourne, Australia.
Green
S L
SL
Rogerson
J
J
Radley
J M
JM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology / Q000502:physiology; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D002450:Cell Communication / Q000502:physiology; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology* / Q000276:immunology / Q000648:ultrastructure; D005260:Female; D006046:Gold; D007150:Immunohistochemistry; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D013997:Time Factors
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D006046:Gold