pHAZE: a shuttle vector system for the detection and analysis of ionizing radiation-induced mutations.
We have designed and constructed a shuttle vector system, pHAZE, that is maintained as an episome in normal human fibroblasts, has a low background mutation frequency, and is capable of detecting a spectrum of mutations, including deletions up to 8.3 kb. The efficacy of this system was demonstrated by using it to analyze mutations produced by X-rays, which induced both point mutations and large deletions in pHAZE.
Lutze
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LH
Laboratory of Radiobiology and Environmental Health, University of California, San Francisco 94143-0750.
Winegar
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RA
D002460:Cell Line; D002872:Chromosome Deletion; D002999:Clone Cells; D004247:DNA / Q000528:radiation effects*; D005822:Genetic Vectors; D006801:Humans; D009154:Mutation; D010957:Plasmids; D012150:Polymorphism, Restriction Fragment Length
D004247:DNA