title: Monitoring of cyclosporine concentrations by using dry blood-spot samples.
abstract: We modified the Incstar Cyclo Trac SP kit to enable its use with dry blood-spots on filter paper. The recovery ranged from 92 to 106%. Dilution studies have shown excellent linearity and parallelism throughout the range of the assay. Precision is demonstrated by within-assay CV's of 6.6 and 4.3% at 96 and 342 micrograms/L respectively and between-assay CV's of 9.1 and 7.0% at 138 and 506 micrograms/L respectively. A comparison study (n = 209) with whole blood assay gave a correlation coefficient of 0.97, a slope of 1.04, and an intercept of 13.2. Whole blood and dry blood-spot cyclosporine assays on heart, kidney, liver, and lung transplants were also compared.
authors.affiliation: Department of Clinical Biochemistry, Toronto Hospital, Ontario, Canada.
authors.forename: A V
authors.initials: AV
authors.lastname: Mee
authors.forename: P Y
authors.initials: PY
authors.lastname: Wong
authors.forename: C
authors.initials: C
authors.lastname: Sun
authors.forename: L
authors.initials: L
authors.lastname: Oei
authors.forename: S
authors.initials: S
authors.lastname: Elliott
authors.forename: N
authors.initials: N
authors.lastname: Naik
authors.forename: B
authors.initials: B
authors.lastname: Joaquin
authors.forename: D
authors.initials: D
authors.lastname: Uchimaru
mesh_terms: D003524:Cyclosporins / Q000097:blood* / Q000592:standards; D005069:Evaluation Studies as Topic; D006801:Humans; D016377:Organ Transplantation; D011863:Radioimmunoassay / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data
chemical_list: D003524:Cyclosporins