Immunocytochemistry of thyroid C-cell complexes in dogs.
Ten thyroid C-cell complexes from five male and five female Beagle dogs, 1 to 2 years old, were studied using single and double immunocytochemical staining with the peroxidase-antiperoxidase technique. The antigens tested included thyroglobulin, calcitonin, calcitonin gene-related peptide, somatostatin, neuron specific enolase, and neurotensin. All C-cell complexes contained four cell types in various proportions: 1) follicular cells staining for thyroglobulin; 2) C-cells staining for calcitonin, calcitonin gene-related peptide, and neuron specific enolase; 3) stellate cells and cuboidal cells in follicle-like structures staining positively for somatostatin; and 4) undifferentiated cells staining negatively for all antigens. No positive immunoreactivity for neurotensin was detected. These findings support the hypothesis that thyroid C-cell complexes are ultimobranchial remnants that can give rise to thyroid follicles and C-cells.
Leblanc
B
B
Department of Pathology, Pfizer Laboratories, Amboise, France.
Paulus
G
G
Andreu
M
M
Bonnet
M C
MC
D000818:Animals; D002116:Calcitonin / Q000032:analysis; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D004285:Dogs / Q000033:anatomy & histology*; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D009496:Neurotensin / Q000032:analysis; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D013004:Somatostatin / Q000032:analysis; D013961:Thyroid Gland / Q000737:chemistry / Q000166:cytology*
D009496:Neurotensin; D013004:Somatostatin; D002116:Calcitonin; D010751:Phosphopyruvate Hydratase; D015740:Calcitonin Gene-Related Peptide