title: Detection by denaturing gradient gel electrophoresis of a new polymorphism in the apolipoprotein B gene.
abstract: The apolipoprotein B gene is subject to mutations that may be important in coronary heart diseases. We have used polymerase chain reaction and denaturing gradient gel electrophoresis to characterize a single nucleotide substitution in the apolipoprotein B gene. This mutation affects amino acid 4311 of the protein and converts asparagine to serine. It was found in 24% of the 81 unrelated individuals analyzed. Moreover, another mutation was detected by sequencing in a single individual.
authors.affiliation: CNRS UPR 8402, Institut de Biologie, Montpellier, France.
authors.forename: M
authors.initials: M
authors.lastname: Navajas
authors.forename: A M
authors.initials: AM
authors.lastname: Laurent
authors.forename: J F
authors.initials: JF
authors.lastname: Moreel
authors.forename: A
authors.initials: A
authors.lastname: Ragab
authors.forename: J P
authors.initials: JP
authors.lastname: Cambou
authors.forename: G
authors.initials: G
authors.lastname: Cuny
authors.forename: F
authors.initials: F
authors.lastname: Cambien
authors.forename: G
authors.initials: G
authors.lastname: Roizès
mesh_terms: D001055:Apolipoproteins B / Q000097:blood / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D001055:Apolipoproteins B; D015345:Oligonucleotide Probes; D004247:DNA