Bacterial killing in vitro by abscess-derived neutrophils.
In the absence of antimicrobial therapy, bacteria such as Bacteriodes fragilis, Escherichia coli and Proteus mirabilis may persist within an intra-abdominal abscess in the presence of large numbers of neutrophils which, under optimal conditions in vitro, can readily phagocytose and kill the same bacterial strains. Neutrophils taken from abscesses induced by gram-negative bacteria such as those above contain viable organisms. On incubation in vitro in the presence of serum, these neutrophils kill the bacteria phagocytosed in the abscess poorly, if at all, yet can readily kill organisms added in vitro. To determine possible mechanisms that might explain this, we examined the bactericidal activity in vitro of neutrophils from a range of abscesses induced by one or two species of bacteria plus an abscess-potentiating agent, bran. The organisms studied were B. fragilis, E. coli, P. mirabilis and Staphylococcus aureus. The killing in vitro of E. coli and P. mirabilis, engulfed within an abscess, was significantly less than that of the same organisms when they were added to the in-vitro assay. In contrast, the killing of S. aureus was similar, whether engulfed in vivo or in vitro. However, S. aureus was less susceptible to phagocytosis and killing in vitro than P. mirabilis or E. coli, and the killing of S. aureus during in-vitro incubation of neutrophils that had engulfed the organism with in the abscess was similar to that of the gram-negative bacteria engulfed within the abscess.(ABSTRACT TRUNCATED AT 250 WORDS)
Finlay-Jones
J J
JJ
Department of Clinical Microbiology, School of Medicine, Flinders University of South Australia, Adelaide.
Hart
P H
PH
Spencer
L K
LK
Nulsen
M F
MF
Kenny
P A
PA
McDonald
P J
PJ
D000038:Abscess / Q000276:immunology* / Q000382:microbiology; D000818:Animals; D001442:Bacteroides Infections / Q000276:immunology / Q000382:microbiology; D001441:Bacteroides fragilis / Q000276:immunology*; D001770:Blood Bactericidal Activity; D004755:Enterobacteriaceae / Q000276:immunology*; D004756:Enterobacteriaceae Infections / Q000276:immunology / Q000382:microbiology; D016177:Macrophage-1 Antigen / Q000032:analysis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000276:immunology*; D010587:Phagocytosis; D011961:Receptors, Fc / Q000032:analysis; D013203:Staphylococcal Infections / Q000276:immunology / Q000382:microbiology; D013211:Staphylococcus aureus / Q000276:immunology*
D016177:Macrophage-1 Antigen; D011961:Receptors, Fc