Structure and chromosome assignment of human S100 alpha and beta subunit genes.
The human S-100 alpha and beta subunit genes were isolated from human genomic DNA library. The restriction endonuclease mapping and DNA sequencing analysis revealed that both subunit genes consisted of three exons and two introns. Two Ca2(+)-binding domains were independently encoded by exon 2 and exon 3. Spot-blot hybridization analysis with flow-sorted chromosomes showed that human alpha and beta subunit genes were assigned to chromosome 1 and chromosome 21, respectively.
Morii
K
K
Research Laboratory for Molecular Genetics, Niigata University, Japan.
Tanaka
R
R
Takahashi
Y
Y
Minoshima
S
S
Fukuyama
R
R
Shimizu
N
N
Kuwano
R
R
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D002891:Chromosomes, Human, Pair 21; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005091:Exons; D015723:Gene Library; D006801:Humans; D007438:Introns; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D009418:S100 Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D046911:Macromolecular Substances; D009418:S100 Proteins; D004247:DNA