title: Amyloid fibril formation in the rough endoplasmic reticulum of plasma cells from a patient with localized A lambda amyloidosis.
abstract: Evidence for amyloid fibril formation in rough endoplasmic reticulum (RER) of plasma cells from the duodenum of a 62-year-old man with localized A lambda amyloidosis is described. The inclusions in RER of plasma cells were composed of tightly packed, regular arrays of fibrils cut in both longitudinal and cross-sections. The fibrils within the inclusions, measuring 10 nm in width, were oriented parallel to the long axis of the inclusions. By immunoelectron microscopy with an antihuman A lambda antiserum, gold particles labeled the fibrils located both in the RER of plasma cells and in the extracellular space. In addition, electron-dense material in the dilated RER was occasionally labeled. These findings suggest that at least some amyloid fibrils are unequivocally created in the RER of plasma cells.
authors.affiliation: First Department of Pathology, Yamaguchi University School of Medicine, Ube, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ishihara
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: M
authors.initials: M
authors.lastname: Koga
authors.forename: T
authors.initials: T
authors.lastname: Yokota
authors.forename: Y
authors.initials: Y
authors.lastname: Yamashita
authors.forename: F
authors.initials: F
authors.lastname: Uchino
mesh_terms: D000682:Amyloid / Q000378:metabolism / Q000648:ultrastructure*; D000686:Amyloidosis / Q000473:pathology*; D001853:Bone Marrow / Q000473:pathology; D004386:Duodenum / Q000473:pathology; D004721:Endoplasmic Reticulum / Q000648:ultrastructure*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D010950:Plasma Cells / Q000473:pathology / Q000648:ultrastructure*; D012007:Rectum / Q000473:pathology; D012867:Skin / Q000473:pathology; D013270:Stomach / Q000473:pathology
chemical_list: D000682:Amyloid