Colonial variation and fimbriation of Actinobacillus actinomycetemcomitans.
Three colonial variants of Actinobacillus actinomycetemcomitans, which formed transparent rough (TR)-, transparent smooth (TS)-, and opaque smooth (OS)-surfaced colonies, were described in relation to their fimbriation. TR- and TS-cells were adhesive to agar and glass surfaces but not the OS-cells. The examination by electron microscopy revealed that TR-cells were highly fimbriated but not TS- and OS-cells. Thus, TS-cells seemed to be an intermediate type. The fimbriae were isolated from TR-cells by suspending in 0.15 M ethanolamine-HCl buffer (pH 10.5) and purified by dissolving non-fimbrial components in 0.5% deoxycholate and 0.7% n-octyl-beta-D-glucopyranoside. The relative molecular mass of the fimbrial subunit protein was 54,000.
Inouye
T
T
Department of Microbiology, Okayama University Dental School, Japan.
Ohta
H
H
Kokeguchi
S
S
Fukui
K
K
Kato
K
K
D000188:Actinobacillus / Q000166:cytology* / Q000254:growth & development / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000032:analysis; D015169:Colony Count, Microbial; D010861:Fimbriae, Bacterial / Q000032:analysis / Q000648:ultrastructure*; D015151:Immunoblotting; D008854:Microscopy, Electron; D008970:Molecular Weight; D010641:Phenotype
D001426:Bacterial Proteins