title: Moist heat intraluminal disinfection of CAPD connectors.
abstract: A moist heat technique for disinfecting the inner lumen of commercially available connectology used in the exchange process for Continuous Ambulatory Peritoneal Dialysis (CAPD) was evaluated. Moist heat was generated by a device (PDM-1) that directed microwave energy to heat a sample solution containing a concentration of 10(6) microorganisms inside a pair of mated plastic CAPD connectors. Microorganisms tested included those most prevalent and most problematic in causing peritonitis. Testing, performed according to F.D.A. approved standards, involved heating a sample solution and then placing the sample solution into vials which were then sealed and incubated. Absolute determination of growth versus no growth was measured by macroscopic observation. Positive control samples were performed in the same manner but were not exposed to heat. Negative controls were performed in the same manner in the absence of test organisms. At temperatures of approximately 100 degrees C a D-value of 6.6 seconds was determined using the organism found the most thermoresistant. A cycle time of 54 seconds appeared sufficient to achieve a 10(6) population reduction of all microorganisms tested. The moist heat technique offers a safe, effective method for disinfection of the inner lumen of CAPD connectors.
authors.affiliation: University of Lowell, MA.
authors.forename: S L
authors.initials: SL
authors.lastname: Fessia
authors.forename: R S
authors.initials: RS
authors.lastname: Grabowy
authors.forename: G G
authors.initials: GG
authors.lastname: Bousquet
mesh_terms: D001234:Aspergillus niger; D002408:Catheters, Indwelling; D004203:Disinfection / Q000379:methods*; D004867:Equipment Design; D005069:Evaluation Studies as Topic; D006358:Hot Temperature; D006801:Humans; D008872:Microwaves; D010531:Peritoneal Dialysis, Continuous Ambulatory / Q000295:instrumentation*; D010538:Peritonitis / Q000517:prevention & control; D013212:Staphylococcus epidermidis