title: Isolation of a cDNA probe for the human intestinal dipeptidylpeptidase IV and assignment of the gene locus DPP4 to chromosome 2.
abstract: We report the nucleotide sequence and derived amino-acid sequence of a cDNA clone encoding the 3' end of human intestinal dipeptidylpeptidase IV (DPP-IV). This cDNA probe identifies a 4 kb mRNA in the human colon cancer cell line Caco-2. We demonstrate here an extensive homology between this human DPP-IV cDNA and the recently published rat liver DPP-IV cDNA. Using the human DPP-IV cDNA to probe genomic DNA from a panel of somatic cell hybrids we have assigned the gene encoding human DPP-IV to chromosome 2.
authors.affiliation: Unité de Recherche sur la Différenciation et la Neuroendocrinologie de Cellules Digestives, INSERM U178, Villejuif, France.
authors.forename: D
authors.initials: D
authors.lastname: Darmoul
authors.forename: M
authors.initials: M
authors.lastname: Lacasa
authors.forename: I
authors.initials: I
authors.lastname: Chantret
authors.forename: D M
authors.initials: DM
authors.lastname: Swallow
authors.forename: G
authors.initials: G
authors.lastname: Trugnan
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D015342:DNA Probes / Q000302:isolation & purification*; D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
chemical_list: D015342:DNA Probes; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D018819:Dipeptidyl Peptidase 4