Identification of beta 2-adrenoceptors on guinea pig alveolar macrophages using (-)-3-[125I]iodocyanopindolol.
The beta-adrenoceptor antagonist (-)-3-[125I]iodocyanopindolol ([125I]ICYP) binds with high affinity and in saturable way to membranes of guinea pig alveolar macrophages. The equilibrium dissociation constant for [125I]ICYP is 24.3 +/- 1.2 pM, and the number of binding sites is 166.3 +/- 13.7 fmol/mg protein (N = 4, +/- SEM). Displacement studies with selective antagonists showed that [125I]ICYP labels beta 2-adrenoceptors on guinea pig alveolar macrophages.
Leurs
R
R
Department of Pharmacochemistry, Faculty of Chemistry, Vrije Universiteit, Amsterdam, The Netherlands.
Beusenberg
F D
FD
Bast
A
A
Van Amsterdam
J G
JG
Timmerman
H
H
D000319:Adrenergic beta-Antagonists / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive; D002462:Cell Membrane / Q000648:ultrastructure; D006168:Guinea Pigs; D007457:Iodine Radioisotopes; D020120:Iodocyanopindolol; D008264:Macrophages / Q000648:ultrastructure*; D008297:Male; D010869:Pindolol / Q000031:analogs & derivatives / Q000378:metabolism; D011412:Propanolamines / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000166:cytology; D011943:Receptors, Adrenergic, beta / Q000145:classification / Q000378:metabolism*; D013999:Timolol / Q000378:metabolism
D000319:Adrenergic beta-Antagonists; D007457:Iodine Radioisotopes; D011412:Propanolamines; D011943:Receptors, Adrenergic, beta; C026777:ICI 118551; D013999:Timolol; D020120:Iodocyanopindolol; D010869:Pindolol; C025716:ICI 89406