Monoclonal antibodies to oxytocin: production and characterization.
To induce a good immune response to oxytocin (OT) we developed a two-step technique to conjugate OT to thyroglobulin (TG) using glutaraldehyde. We obtained 30 hybridomas recognizing OT-ovalbumin conjugates and 16 stable lines. Three monoclonal antibodies were selected for further characterization. One of them (O13) was found very specific for OT using three different techniques (enzyme-linked immunosorbent assay, radioimmunoassay and immunohistochemistry); it is directed to the C-terminal tripeptide. The other two probably recognize tyrosine containing epitope(s) also shared by vasopressin and other related nonapeptides.
Burgeon
E
E
Laboratory of Physiology, U.L.B., Rhode-St-Genèse, Belgium.
Chapleur
M
M
Schoenen
J
J
Remichius
D
D
Legros
J J
JJ
Geenen
V
V
Robert
F
F
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006825:Hybridomas / Q000276:immunology; D007031:Hypothalamus / Q000737:chemistry; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D010121:Oxytocin / Q000032:analysis / Q000276:immunology*; D013329:Structure-Activity Relationship; D014667:Vasopressins / Q000032:analysis
D000911:Antibodies, Monoclonal; D014667:Vasopressins; D010121:Oxytocin