title: Five year prospective study of HIV infection in the Edinburgh haemophiliac cohort.
abstract: OBJECTIVE
  To identify measures of immune state that reflect the course of HIV related disease in order to predict deterioration of symptoms and assess response to treatment.
  
  
  METHODS
  Five year longitudinal clinical and laboratory study.
  
  
  METHODS
  Regional haemophilia centre, university virology laboratory, and Medical Research Council laboratory.
  
  
  METHODS
  32 Patients with haemophilia A exposed to a single batch of HIV contaminated factor VIII concentrate from the Scottish National Blood Transfusion Service in 1984 who were followed up regularly in Edinburgh (31) or abroad (one).
  
  
  METHODS
  Counts of circulating T cell subsets (CD4 and CD8); plasma beta 2 microglobulin, neopterin, and IgA concentrations; and delayed type hypersensitivity to multiple skin test antigens.
  
  
  RESULTS
  18 Patients who seroconverted after exposure had received significantly more contaminated factor VIII than the 14 who did not (mean 43 (range 9-109) v 15 (3-30) phials, p less than 0.01). The two groups were not distinguishable by other criteria before exposure. The group that seroconverted subsequently showed a progressive fall in mean circulating CD4 lymphocytes and an increase in plasma beta 2 microglobulin and neopterin concentrations. From 1987 patients in this group also showed an increase in mean circulating CD8 lymphocytes and in plasma IgA concentration, neither of which was seen in patients who did not seroconvert. Patients with HIV antibody who developed Centers for Disease Control category IV symptoms within five years after infection showed more extreme changes in all measures, except CD8 lymphocyte count, than those whose symptoms remained in categories II and III. Skin test reactivity declined to barely detectable levels in all patients positive for HIV antibody.
  
  
  CONCLUSIONS
  Serial estimates of circulating CD4 lymphocytes and of plasma beta 2 microglobulin concentration are the most reliable measures of disease progression; of these, beta 2 microglobulin concentration seems to be the better predictor of impending serious symptoms. High IgA concentrations reflect rather than predict disease state. Individual variation in most measures is such that a wide range of measurements should be used in assessing the effects of trial treatment in HIV infected patients without symptoms.
authors.affiliation: Department of Haematology, Royal Infirmary of Edinburgh.
authors.forename: R J
authors.initials: RJ
authors.lastname: Cuthbert
authors.forename: C A
authors.initials: CA
authors.lastname: Ludlam
authors.forename: J
authors.initials: J
authors.lastname: Tucker
authors.forename: C M
authors.initials: CM
authors.lastname: Steel
authors.forename: D
authors.initials: D
authors.lastname: Beatson
authors.forename: S
authors.initials: S
authors.lastname: Rebus
authors.forename: J F
authors.initials: JF
authors.lastname: Peutherer
mesh_terms: D000293:Adolescent; D000328:Adult; D001708:Biopterins / Q000031:analogs & derivatives / Q000097:blood; D015496:CD4-Positive T-Lymphocytes; D015658:HIV Infections / Q000209:etiology / Q000276:immunology*; D006679:HIV Seropositivity / Q000276:immunology; D006467:Hemophilia A / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D019798:Neopterin; D011446:Prospective Studies; D012606:Scotland; D050378:T-Lymphocytes, Regulatory; D001613:beta 2-Microglobulin / Q000378:metabolism
chemical_list: D007070:Immunoglobulin A; D001613:beta 2-Microglobulin; D001708:Biopterins; D019798:Neopterin