Effects of histamine on protein and glycoprotein production of isolated pig gastric mucosal cells.
The production of glycoprotein and protein by isolated pig gastric non-parietal cells was measured by incorporation of N-acetyl-[14C]-D-glucosamine [( 14C]GlcNAc) and [3H]-L-leucine [( 3H]Leu), respectively, into acid insoluble material (AIM). Histamine enhanced incorporation of the tracers into cellular and released AIM in a concentration-dependent manner. The H2 receptor antagonist ranitidine completely blocked the effects of histamine (100 mumol/l) on [3H]Leu incorporation into cellular and released AIM (IC50 37 and 32 mumol/l, respectively) but had no inhibitory effect on the 16,16-dimethylprostaglandin-E2 - and forskolin-stimulated tracer incorporation. The H1 receptor antagonist mepyramine did not inhibit the histamine effect. We conclude that histamine is a stimulant of protein, via H2 receptors, and glycoprotein production of isolated pig gastric non-parietal cells.
Heim
H K
HK
Abteilung Allgemeine Pharmakologie, Medizinische Hochschule Hannover, FRG.
Oestmann
A
A
Sewing
K F
KF
D015064:16,16-Dimethylprostaglandin E2 / Q000494:pharmacology; D000117:Acetylglucosamine / Q000378:metabolism; D000818:Animals; D005576:Colforsin / Q000494:pharmacology; D005753:Gastric Mucosa / Q000166:cytology / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis*; D006632:Histamine / Q000494:pharmacology*; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D007930:Leucine / Q000378:metabolism; D014176:Protein Biosynthesis; D011738:Pyrilamine / Q000494:pharmacology; D011899:Ranitidine / Q000494:pharmacology; D011969:Receptors, Histamine H1 / Q000187:drug effects; D011970:Receptors, Histamine H2 / Q000187:drug effects; D013552:Swine; D014316:Tritium
D006023:Glycoproteins; D006634:Histamine H1 Antagonists; D006635:Histamine H2 Antagonists; D011969:Receptors, Histamine H1; D011970:Receptors, Histamine H2; D014316:Tritium; D005576:Colforsin; D006632:Histamine; D011899:Ranitidine; D007930:Leucine; D011738:Pyrilamine; D015064:16,16-Dimethylprostaglandin E2; D000117:Acetylglucosamine