title: Stimulation of neurite outgrowth in neuroblastoma cells by neuraminidase: putative role of GM1 ganglioside in differentiation.
abstract: Treatment of three neuroblastoma cell types in culture with neuraminidase resulted in enhanced neurite outgrowth. These included the mouse Neuro-2A and rat B104 and B50 lines. The morphological changes depended on the presence of exogenous Ca2+ and were accompanied by modest but statistically significant increases in 45Ca2+ influx. Neuraminidase-stimulated neuritogenesis was blocked by the B subunit of cholera toxin (cholera B) and anti-GM1 antibody, a finding suggesting the effect was due to an increased amount of GM1 on the cell surface. Cholera B also blocked the increase in 45Ca2+ influx. The mouse N1A-103 line, previously characterized as "neurite minus," did not respond to neuraminidase with either neurite outgrowth or enhanced Ca2+ influx. These results point to an influence of GM1 on neuritogenesis in cells with differentiation potential and suggest a mechanism involving modulation of Ca2+ flux.
authors.affiliation: Department of Neurology, Albert Einstein College of Medicine, Bronx, New York 10461.
authors.forename: G
authors.initials: G
authors.lastname: Wu
authors.forename: R W
authors.initials: RW
authors.lastname: Ledeen
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001369:Axons / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D002118:Calcium / Q000378:metabolism; D002132:Calcium Radioisotopes; D002772:Cholera Toxin / Q000494:pharmacology; D005677:G(M1) Ganglioside / Q000276:immunology / Q000502:physiology*; D007811:Lanthanum / Q000494:pharmacology; D051379:Mice; D009439:Neuraminidase / Q000494:pharmacology*; D009447:Neuroblastoma / Q000648:ultrastructure*; D010446:Peptide Fragments / Q000494:pharmacology; D051381:Rats; D014407:Tumor Cells, Cultured
chemical_list: D000906:Antibodies; D002132:Calcium Radioisotopes; D010446:Peptide Fragments; C028521:lanthanum chloride; D005677:G(M1) Ganglioside; D007811:Lanthanum; D002772:Cholera Toxin; D009439:Neuraminidase; D002118:Calcium