title: Widespread distribution of the 14-3-3 protein in vertebrate brains and bovine tissues: correlation with the distributions of calcium-dependent protein kinases.
abstract: A highly specific antiserum was prepared against bovine brain 14-3-3 protein, a protein kinase-dependent activator of tyrosine and tryptophan hydroxylases. The immunoassay using this antiserum proved the presence of 14-3-3 protein in various bovine tissues and in brains of various vertebrate species. The quantitative analysis indicated that the tissue distribution of 14-3-3 protein is more closely related to the known distributions of the Ca2(+)-dependent protein kinases, i.e., Ca2+/calmodulin- and Ca2+/phospholipid-dependent protein kinases, rather than those of tyrosine and tryptophan hydroxylases. This result, together with the available data on this protein, suggests potential roles of the 14-3-3 protein in more diverse kinase-mediated processes than the predicted role in monoamine synthesis.
authors.affiliation: Department of Biosystem Science, Graduate School of Science and Technology, Niigata University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ichimura
authors.forename: H
authors.initials: H
authors.lastname: Sugano
authors.forename: R
authors.initials: R
authors.lastname: Kuwano
authors.forename: T
authors.initials: T
authors.lastname: Sunaya
authors.forename: T
authors.initials: T
authors.lastname: Okuyama
authors.forename: T
authors.initials: T
authors.lastname: Isobe
mesh_terms: D048948:14-3-3 Proteins; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D007120:Immunochemistry; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism*; D011817:Rabbits; D014018:Tissue Distribution; D014446:Tyrosine 3-Monooxygenase
chemical_list: D048948:14-3-3 Proteins; D009419:Nerve Tissue Proteins; D014446:Tyrosine 3-Monooxygenase; D011494:Protein Kinases