In vitro development of exoerythrocytic forms of Plasmodium gallinaceum sporozoites in avian macrophages.
Exoerythrocytic forms of Plasmodium gallinaceum were cultured in vitro using salivary gland sporozoites extracted from experimentally infected Aedes fluviatilis mosquitoes. The host cells were macrophage precursors from chicken bone marrow. At various times after introduction of sporozoites, the cultures were stained by Giemsa or by immunofluorescence assay (IFA) using anti-sporozoite-specific monoclonal antibodies (MAb). The time to complete parasite development in vitro was 50-70 h. By 70 h, ruptured segmenters and free merozoites were visible within the cells. Inoculation of normal chickens with infected cultures induced parasitemia after a pre-patent period of 10-11 days. In vitro young exoerythrocytic forms, late schizonts that include the matured segmenters, and free merozoites shared common antigens with the sporozoites as revealed by IFA using anti-sporozoite-specific MAbs. Our data indicate that macrophages support development of P. gallinaceum sporozoites and that the circumsporozoite proteins are present until the end of the primary exoerythrocytic schizogony.
Ramirez
A D
AD
Departamento de Parasitologia, Universidade Federal de Minas Gerais, Brazil.
Rocha
E M
EM
Krettli
A U
AU
D000818:Animals; D000911:Antibodies, Monoclonal; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D002645:Chickens; D005455:Fluorescent Antibody Technique; D008264:Macrophages / Q000469:parasitology*; D008856:Microscopy, Fluorescence; D010964:Plasmodium gallinaceum / Q000254:growth & development*
D000911:Antibodies, Monoclonal