Whole body hyperthermia: a potent radioprotector in vivo.
Interleukin-1 has been reported to be an effective radioprotective agent in mice subjected to lethal doses of irradiation. Production of Interleukin-1 can be increased by whole body hyperthermia. Therefore, whole body hyperthermia was assessed for its efficacy in protecting the lethal effects of ionizing radiation in DBA/2 mice. One hour of 40 degrees C +/- 0.2 whole body hyperthermia given 20 hr before 900 cGy total body irradiation protected 100% of DBA/2 mice from an LD 100/16 radiation dose (dose of irradiation that killed 100% of the mice in 16 days). Lethal doses of total body irradiation produced profound monocytopenia, decreased cellularity of thymus, spleen, and bone marrow, and suppressed Interleukin-1 production. Interleukin-1 production was determined using the thymocyte proliferation assay. Whole body hyperthermia accelerated recovery of blood leukocytes by up to 5 days post-total body irradiation in DBA/2 mice. Thymocytes, spleen, and bone marrow cells were activated by whole body hyperthermia, as assessed by the cell's response to Concanavalin A. This was accompanied by accelerated Interleukin-1 generation. Our results provide the first evidence that whole body hyperthermia acts as a potent radioprotector in vivo, effects that may be mediated by Interleukin-1.
Shen
R N
RN
Dept. of Medicine (Hematology/Oncology), Indiana University School of Medicine, Indianapolis 46202.
Hornback
N B
NB
Shidnia
H
H
Wu
B
B
Lu
L
L
Broxmeyer
H E
HE
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000528:radiation effects; D005260:Female; D006979:Hyperthermia, Induced / Q000379:methods*; D007375:Interleukin-1 / Q000096:biosynthesis; D007958:Leukocyte Count / Q000528:radiation effects; D008213:Lymphocyte Activation / Q000528:radiation effects; D008214:Lymphocytes / Q000378:metabolism; D051379:Mice; D008811:Mice, Inbred DBA; D011829:Radiation Dosage; D011835:Radiation Protection; D014916:Whole-Body Irradiation / Q000009:adverse effects* / Q000401:mortality
D007375:Interleukin-1