Genetic mapping with dispersed repeated sequences in the rice blast fungus: mapping the SMO locus.
The SMO genetic locus in strains of the fungus Magnaporthe grisea that infect weeping lovegrass, directs the formation of correct cell shapes in asexual spores, infection structures, and asci. We have identified and characterized a Smo- strain of M. grisea that infects rice. The smo mutation in this strain segregates as a single gene mutation and is allelic to previously identified smo alleles. A marked reduction in pathogenicity co-segregates with the Smo- phenotype, suggesting that the SMO locus plays a role in rice pathogenicity. A family of dispersed repeated DNA sequences, called MGR, have been discovered in the nuclear DNA of M. grisea rice pathogens. Genetic crosses between Smo- rice pathogens and Smo+ non-rice pathogens were used to follow the segregation of the SMO locus and individual MGR sequences. Using DNA blot analysis with cloned MGR hybridization probes, we mapped the SMO locus to a chromosomal region flanked by two closely linked MGR sequences. We demonstrated that the copy number of MGR sequences could be reduced in subsequent crosses to non-rice pathogens of M. grisea, and that new MGR sequences did not occur following meiosis indicating that these sequences are stable in the genome. We conclude that restriction fragment polymorphism mapping with cloned MGR sequences as hybridization probes is an effective way to map genes in the rice blast fungus.
Hamer
J E
JE
Department of Biological Sciences, Purdue University, West Lafayette, IN 47906.
Givan
S
S
D001203:Ascomycota / Q000235:genetics*; D003433:Crosses, Genetic; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D009154:Mutation; D012275:Oryza / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D004271:DNA, Fungal