Determination of nizatidine and two of its main metabolites in human serum using high-performance liquid chromatography.
A high-performance liquid chromatographic assay has been developed for the determination of nizatidine, a new histamine H2-receptor antagonist, and two of its main metabolites, N-desmethylnizatidine and nizatidine sulphoxide. Drugs were extracted with chloroform-2-propanol (90:10, v/v) from alkalinized samples of serum, using ranitidine as an internal standard. After evaporation of the extraction solvent, the residue was removed and analysed on a LiChrosorb Si60 5-microns column with a mobile phase of acetonitrile-methanol-water-ammonia solution (1000:200:20:5, v/v). The compounds were detected at 320 nm. The lower detection limits were 6-18 ng/ml at a signal-to-noise ratio of 3. This method is simple and specific, and the single-step extraction makes it rapid. It is the first high-performance liquid chromatographic assay to be described for the determination of nizatidine metabolites.
Tracqui
A
A
Institut de Médecine Légale, Faculté de Médecine de Strasbourg, France.
Kintz
P
P
Mangin
P
P
D002851:Chromatography, High Pressure Liquid; D006635:Histamine H2 Antagonists / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016567:Nizatidine / Q000031:analogs & derivatives; D011899:Ranitidine / Q000097:blood; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013844:Thiazoles / Q000097:blood* / Q000493:pharmacokinetics
D006635:Histamine H2 Antagonists; D012997:Solvents; D013844:Thiazoles; C066300:nizatidine sulfoxide; C066301:N-desmethylnizatidine; D011899:Ranitidine; D016567:Nizatidine