Establishment of dose-response relationships between doses of Cs-137 gamma-rays and frequencies of micronuclei in human peripheral blood lymphocytes.
It has been suggested that the yield of micronuclei in human peripheral blood lymphocytes could be used as a biological dosimeter in cases of radiation exposure. In the present study micronuclei were induced in lymphocytes by exposing human blood samples in vitro to various doses of Cs-137 gamma-rays. The blood samples were then cultivated using the cytokinesis block method. Coded programs were employed to establish the relationships between the frequencies of micronuclei and various doses of gamma-rays. The best fit was obtained by the linear-quadratic model, Y = c + aD + bd2, where Y is the yield of micronuclei, D is the dose in Gy and c, a, b, are constants. It seems there is a correlation between the yields of MN in mononuclear cells and the corresponding doses of radiation. Therefore an attempt was made to include these MN in the calculation of the dose-response relationship.
Balasem
A N
AN
Department of Health Physics, Nuclear Research Center, Tuwitha, Baghdad, Iraq.
Ali
A S
AS
D000328:Adult; D002455:Cell Division / Q000528:radiation effects; D002478:Cells, Cultured; D002588:Cesium Radioisotopes / Q000009:adverse effects; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays / Q000009:adverse effects*; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000166:cytology / Q000528:radiation effects*; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000528:radiation effects*
D002588:Cesium Radioisotopes