The octamer binding factor Oct6: cDNA cloning and expression in early embryonic cells.
We have cloned a cDNA encoding a novel octamer binding factor Oct6 that is expressed in undifferentiated ES cells. Expression of the Oct6 gene is downregulated upon differentiation of these cells by aggregate formation. Furthermore the gene is transiently up regulated during retinoic acid induced differentiation of P19 EC cells, reaching maximum levels of expression one day after RA addition. Sequence analysis of the cDNA encoding the Oct6 protein indicated that the Oct6 gene is a member of the POU-HOMEO domain gene family. The gene expresses a 3 kb mRNA encoding a 449 amino acid protein with an apparent molecular weight of 45 kD. The sequence of the Oct6 POU domain is identical to that of the rat SCIP (Tst-1) gene. The Oct6 expression pattern suggests a role for this DNA binding protein in neurogenesis as well as early embryogenesis.
Meijer
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D
MGC Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands.
Graus
A
A
Kraay
R
R
Langeveld
A
A
Mulder
M P
MP
Grosveld
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002454:Cell Differentiation; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D054278:Embryonal Carcinoma Stem Cells; D005786:Gene Expression Regulation; D005801:Genes, Homeobox; D008969:Molecular Sequence Data; D008970:Molecular Weight; D005810:Multigene Family; D014411:Neoplastic Stem Cells; D050816:Octamer Transcription Factor-6; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology
D004268:DNA-Binding Proteins; D014157:Transcription Factors; D050816:Octamer Transcription Factor-6; D014212:Tretinoin; D004247:DNA