title: Effect of albumin on acyl-CoA: lysolecithin acyltransferase, lysolecithin: lysolecithin acyltransferase and acyl-CoA hydrolase from rabbit lung.
abstract: Acyl-CoA: lysolecithin and lysolecithin: lysolecithin acyltransferases, as well as acyl-CoA hydrolase are important enzymes in lung lipid metabolism. They use amphiphylic lipids as substrates and differ in subcellular localization. In this sense, lipid-protein interactions can be an essential factor in their activity. We have studied the effect of albumin, as lipid-binding protein model, in the activities of these enzymes. Acyl-CoA hydrolase was inhibited in the presence of albumin, whereas acyl-CoA: lysolecithin acyltransferase showed a complex effect of activation depending on both albumin concentration and palmitoyl-CoA/lysolecithin molar ratio. Lysolecithin: lysolecithin acyltransferase was affected differentially on its two activities. Hydrolysis remained unaffected and transacylation was inhibited by albumin. These results are consequence of the interaction of albumin with both lipidic substrates that changes their critical micellar concentration.
authors.affiliation: Departmento de Bioquímica y Biología Molecular I, Facultad de Ciencias Químicas, Universidad Complutense, Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Pérez-Gil
authors.forename: P
authors.initials: P
authors.lastname: Estrada
authors.forename: C
authors.initials: C
authors.lastname: Acebal
authors.forename: R
authors.initials: R
authors.lastname: Arche
mesh_terms: D008243:1-Acylglycerophosphocholine O-Acyltransferase / Q000378:metabolism*; D000215:Acylation; D000217:Acyltransferases / Q000378:metabolism*; D000327:Adsorption; D000418:Albumins / Q000493:pharmacokinetics*; D000818:Animals; D008168:Lung / Q000187:drug effects / Q000201:enzymology*; D008244:Lysophosphatidylcholines / Q000378:metabolism; D010171:Palmitoyl Coenzyme A / Q000378:metabolism; D010170:Palmitoyl-CoA Hydrolase; D011817:Rabbits; D013050:Spectrometry, Fluorescence; D013379:Substrate Specificity
chemical_list: D000418:Albumins; D008244:Lysophosphatidylcholines; D010171:Palmitoyl Coenzyme A; D000217:Acyltransferases; D008243:1-Acylglycerophosphocholine O-Acyltransferase; D010170:Palmitoyl-CoA Hydrolase