Rat urate oxidase: cloning and structural analysis of the gene and 5'-flanking region.
The structural gene (UOX) encoding rat urate oxidase (UOX) spans at least 23 kb and is composed of eight exons and seven introns. All of the exon-intron splice junction sequences conformed to the GT/AG consensus established for eukaryotic genes. The transcription start point (tsp) was determined using S1-type nuclease protection riboprobe, and assigned to an adenine 54 nucleotides (nt) upstream of the ATG start codon. A 456-bp 5'-terminal fragment, starting at the ATG codon, carries a putative TATA (ATAAAA) sequence at -32, and two putative 'CAAT box' sequences at -62 and -71 bp upstream from the tsp. No sequence resembling 'GC' box hexanucleotides (GGGCGG or CCGCCC) was found. The structural features of the 5'-flanking region of the UOX gene are distinct from the 5'-flanking sequences of peroxisomal beta-oxidation system genes which contain one or more 'GC' box elements but lack TATA- and CAAT-like features [Osumi et al., J. Biol. Chem. 262 (1987) 8138-8143; Ishii et al., J. Biol. Chem. 262 (1987) 8144-8150]. The 5'-flanking region of the UOX gene reveals a sequence, TTAGTAATT at nt -276 from the tsp, which appears to be complementary to the underlined part of the liver-specific LF-B1/HNF-1 consensus sequence, GTTAATNATTAAC (where N = A, C, T, G or no nt).
Wang
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XD
Department of Pathology, Northwestern University Medical School, Chicago, IL 60611.
Kawano
H
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Alvares
K
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Reddy
P G
PG
Getto
H
H
Rao
M S
MS
Reddy
J K
JK
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D005091:Exons; D005796:Genes; D007438:Introns; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D012326:RNA Splicing; D051381:Rats; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014503:Urate Oxidase / Q000235:genetics* / Q000378:metabolism
D014503:Urate Oxidase