Sister-chromatid exchange analysis in vivo using different 5-bromo-2'-deoxyuridine-labeling systems.
The quality of sister-chromatid differentiation, the basal rate of sister-chromatid exchanges (SCE) and the rate of cellular proliferation were studied in untreated and mitomycin C(MMC)-treated mice, using 4 different systems for administering 5-bromodeoxyuridine (BrdU): BrdU adsorbed to charcoal, tablets of BrdU mixed with cholesterol, tablets of BrdU coated with agar and tablets of BrdU partially coated with paraffin. The quality of sister-chromatid differentiation with the studied methods showed a useful stain contrast in an average of 75.4% second-division mitosas, with the lowest average occurring in mice implanted with agar-coated tablets. The frequency of SCE and the replicative index were similar in mice administered BrdU by all 4 systems both in control and in MMC-treated mice. From a practical point of view, the charcoal method could be done the fastest.
Madrigal-Bujaidar
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Departamento de Morfología, Escuela Nacional de Ciencias Biológicas, I.P.N. Carpio y Plan de Ayala Col. Santo Tomás, Mexico D.F. Mexico.
Sánchez-Sánchez
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MA
D000818:Animals; D001973:Bromodeoxyuridine; D002455:Cell Division / Q000187:drug effects; D002784:Cholesterol / Q000494:pharmacology / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008937:Mitomycins / Q000494:pharmacology; D008940:Mitotic Index; D009152:Mutagenicity Tests / Q000379:methods*; D012854:Sister Chromatid Exchange
D008937:Mitomycins; D002784:Cholesterol; D001973:Bromodeoxyuridine