Purification of histone H10 and its subfractions under non-denaturing conditions.
A quick and convenient method for a non-denaturing purification of histone H10 and its subfractions from different mammalian sources is presented. It is based on cation-exchange chromatography on a CM-Sephadex C-25 column developed by a linear gradient of NaCl. The procedure allows a satisfactory fractionation of H10 subfractions in a form suitable for further studies in assays requiring the native state of the protein.
Banchev
T
T
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
Srebreva
L
L
Zlatanova
J
J
D000818:Animals; D002852:Chromatography, Ion Exchange / Q000379:methods; D006657:Histones / Q000145:classification / Q000302:isolation & purification*; D006801:Humans; D008099:Liver / Q000737:chemistry; D051379:Mice; D010920:Placenta / Q000737:chemistry; D011489:Protein Denaturation
D006657:Histones