Calcitonin stimulates 1,25-dihydroxyvitamin D production in diabetic rat kidney.
In diabetic animals, there is a decrease in serum 1,25-dihydroxyvitamin D [1,25(OH)2D] and in renal production of 1,25(OH)2D. In nondiabetic animals, renal 1,25(OH)2D production is markedly stimulated by parathyroid hormone (PTH) and calcitonin (CT). There is evidence that diabetes impairs the responsiveness of the kidney to PTH. The effect of diabetes on responsiveness to CT is unknown. The studies reported here determined the effect of streptozotocin-induced diabetes on renal responsiveness to PTH and CT. Experiments were performed in 7- to 8-week-old rats that were fed a diet sufficient in calcium and vitamin D and were thyroparathyroidectomized (TPTX) 5 days before hormone treatment. PTH (0.33 U/g body weight at 24, 12, and 2 hours before death) significantly increased renal 1,25(OH)2D production by threefold in nondiabetic rats. This effect was markedly attenuated by diabetes. On the other hand, CT (20 U/100 g body weight at 12 and 2 hours before death) produced a maximal response in both groups of animals. In diabetic rats, CT stimulated renal 1,25(OH)2D production fivefold, whereas PTH stimulated production only 1.5-fold. Diabetes did not affect the capacity of PTH to increase serum calcium or decrease renal tubular reabsorption of phosphorus (TRP). These findings suggest that the decrease in renal 1,25(OH)2D production seen in experimental diabetes may be due to decreased renal responsiveness to PTH, but not to decreased responsiveness to CT.
Wongsurawat
N
N
Department of Internal Medicine, St. Louis University School of Medicine, MO.
Armbrecht
H J
HJ
D000042:Absorption; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002116:Calcitonin / Q000494:pharmacology*; D002117:Calcitriol / Q000378:metabolism*; D002118:Calcium / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D007684:Kidney Tubules / Q000378:metabolism; D008297:Male; D010281:Parathyroid Hormone / Q000494:pharmacology; D016105:Parathyroidectomy; D010758:Phosphorus / Q000097:blood / Q000493:pharmacokinetics; D051381:Rats; D011916:Rats, Inbred F344; D013965:Thyroidectomy
D001786:Blood Glucose; D010281:Parathyroid Hormone; D010758:Phosphorus; D002116:Calcitonin; D002117:Calcitriol; D002118:Calcium