Mechanisms of prior blood transfusion-cyclosporine-induced tolerance: a potential role for immune-cellular chimerism.
Skin allografts were not enhanced by prior conditioning of blood and CyA (5 or 10 mg/kg/d). However, when BM-CyA pretreatment was used, SA survival was significantly prolonged (CyA, 5 or 10 mg/kg/d). In examining differences between the BT-CyA and BM-CyA protocols, equivocal levels of donor microchimerism (1.5%) were found in the spleens of BT-CyA conditioned recipients at the time of transplantation (day 0). In contrast, highly significant levels of splenic donor chimerism (17.2%) developed at day 0 for the BM-CyA pretransplant recipients. Skin-allograft prolongation under the BM-CyA protocol implied that the effect may be linked to the existence of a donor-specific stem-cell population in the recipient animal.
Beko
K R
KR
Department of Surgery, University of California, Irvine 92717.
Tran
H O
HO
Hewitt
C W
CW
Black
K S
KS
Patel
M P
MP
Ramsamooj
R
R
Martin
D C
DC
D000818:Animals; D001803:Blood Transfusion; D003524:Cyclosporins / Q000627:therapeutic use*; D006085:Graft Survival; D006412:Hematopoietic Stem Cells / Q000276:immunology; D007108:Immune Tolerance; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D016038:Skin Transplantation / Q000276:immunology*; D014184:Transplantation, Homologous
D003524:Cyclosporins