Expression of v-src in a murine T-cell hybridoma results in constitutive T-cell receptor phosphorylation and interleukin 2 production.
Ligand binding to the T-cell antigen receptor results in phosphatidylinositol hydrolysis and the resultant activation of protein kinase C, as well as the activation of a receptor-coupled protein-tyrosine kinase. As a model for tyrosine kinase activation in T cells, we used retroviral gene transfer to express the v-src oncogene in an antigen-specific murine T-cell hybridoma. Clones that expressed v-src mRNA demonstrated constitutive tyrosine phosphorylation of several cellular substrates, including the zeta chain of the T-cell receptor, and constitutive interleukin 2 production. Thus, expression of a constitutively active protein-tyrosine kinase such as pp60v-src appears to be sufficient to induce the expression of at least one gene critical to the process of T-cell activation.
O'Shea
J J
JJ
Biological Response Modifiers Program, PRI/DynCorp, National Cancer Institute-Frederick Cancer Research and Development Center, MD 21701-1201.
Ashwell
J D
JD
Bailey
T L
TL
Cross
S L
SL
Samelson
L E
LE
Klausner
R D
RD
D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D015870:Gene Expression; D016391:Genes, src; D006825:Hybridomas / Q000276:immunology; D007376:Interleukin-2 / Q000096:biosynthesis*; D007700:Kinetics; D051379:Mice; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D012190:Retroviridae / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection
D002087:Butyrates; D007376:Interleukin-2; D011948:Receptors, Antigen, T-Cell; D020148:Butyric Acid; D011505:Protein-Tyrosine Kinases