Adhesive fimbriae produced in vivo by Escherichia coli O139:K12(B):H1 associated with enterotoxaemia in pigs.
Two strains of E. coli O139:K12 (B):H1 were compared in vitro and in the intestinal environment. Both strains colonized the small intestines of experimentally inoculated pigs and exhibited in vivo a similar relationship to the microvillus border as enterotoxigenic E. coli (ETEC). Strain 107/86 grown on blood agar expressed numerous long flexible non-haemagglutinating fimbriae which were antigenically distinct from the known fimbriae of porcine ETEC. It adhered in vitro to porcine enterocyte brush border fragments. Strain 124/76 grown on blood agar was devoid of fimbriae and did not adhere to brush border fragments. However, fimbriae morphologically and antigenically indistinguishable from those of strain 107/86 were detected in the intestinal environment by direct immunofluorescence and by immuno electron microscopy.
Bertschinger
H U
HU
Institute of Veterinary Bacteriology, University of Zürich, Switzerland.
Bachmann
M
M
Mettler
C
C
Pospischil
A
A
Schraner
E M
EM
Stamm
M
M
Sydler
T
T
Wild
P
P
D000818:Animals; D001422:Bacterial Adhesion; D002478:Cells, Cultured; D004926:Escherichia coli / Q000254:growth & development / Q000472:pathogenicity / Q000648:ultrastructure*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D010861:Fimbriae, Bacterial / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D006386:Hemagglutination Tests; D007421:Intestine, Small / Q000382:microbiology; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D008871:Microvilli / Q000382:microbiology; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*