Inhibition of development of Na(+)-dependent hexose transport in renal epithelial LLC-PK1 cells by differentiation-stimulating factor for myeloid leukemic cells/leukemia inhibitory factor.
Differentiation-stimulating factor (D-factor)/leukemia inhibitory factor is a cytokine inducing differentiation of mouse myeloid leukemic M1-T22 cells. The effect of recombinant human D-factor on growth and differentiation of pig kidney LLC-PK1 cells was examined. LLC-PK1 cells did not concentrate alpha-methylglucoside during their early growth in culture but developed the capacity to concentrate this hexose as they reached confluence and their growth rate decreased. Purified D-factor caused dose-dependent inhibition of the development of this concentrative capacity. It did not affect the growth rate of the cells, but inhibited the formation of multicellular domes in confluent cultures. LLC-PK1 cells were found to have high-affinity binding sites (831 per cell) for D-factor with a dissociation constant of 197 pM.
Tomida
M
M
Department of Chemotherapy, Saitama Cancer Center Research Institute, Japan.
Yamamoto-Yamaguchi
Y
Y
Hozumi
M
M
Holmes
W
W
Lowe
D G
DG
Goeddel
D V
DV
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D006131:Growth Inhibitors / Q000494:pharmacology*; D006601:Hexoses / Q000378:metabolism*; D015850:Interleukin-6; D007668:Kidney / Q000166:cytology*; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000378:metabolism; D012964:Sodium / Q000502:physiology*; D013552:Swine; D014882:Water-Electrolyte Balance; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D006131:Growth Inhibitors; D006601:Hexoses; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D011956:Receptors, Cell Surface; D012964:Sodium; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase