The effects of dimethyl sulfoxide on aortic prostacyclin production and serum thromboxane and plasma fibrinogen levels in rabbits fed a normal versus a cholesterol-enriched diet.
Through an unknown mechanism, dimethyl sulfoxide (DMSO) retards atherogenesis in cholesterol-fed rabbits (CFR). We studied the effects on the development of lesions and prostacyclin (PGI2) production in the thoracic aorta and total serum lipid and cholesterol content of the abdominal aortic serum thromboxane (TXB2) and plasma fibrinogen levels in rabbits fed control versus atherogenic diets, with and without DMSO. Without DMSO, PGI2 production was significantly higher in CFR versus control animals (8.65 +/- 1.0 vs 6.38 +/- 0.3 ng/15 min [p less than 0.02]). DMSO did not influence PGI2 production in any of the groups but significantly reduced the number of atheromatous lesions in CFR (78% +/- 9% vs 8% +/- 4% [p less than 0.001]). With DMSO, CFR had a significant reduction in total lipid levels (422 +/- 5 vs 300 +/- 21 mg/gm dry wt [p less than 0.01]) and cholesterol levels (74 +/- 12.8 vs 31.8 +/- 6.4 mg/gm dry wt [p less than 0.01]) compared with control animals. Fibrinogen levels were significantly lower in CFR versus control animals (0.83 +/- 0.07 vs 2.42 +/- 0.13 mg/ml [p less than 0.01]). TXB2 was lower in DMSO plus control versus control animals alone. In conclusion, DMSO does not appear to act through changes in PGI2 or fibrinogen activity. Its effect in lowering TXB2 in CFR suggests an action on platelet function.
Hoover
E L
EL
Department of Surgery, Meharry Medical College, Nashville, Tenn, 37208.
Ross
M J
MJ
Fani
K
K
Adams
C Z
CZ
Debons
A F
AF
D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects* / Q000378:metabolism*; D001161:Arteriosclerosis / Q000188:drug therapy*; D002784:Cholesterol / Q000097:blood; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D004036:Diet, Atherogenic; D004121:Dimethyl Sulfoxide / Q000627:therapeutic use*; D004326:Drinking / Q000187:drug effects; D011464:Epoprostenol / Q000096:biosynthesis*; D005340:Fibrinogen / Q000378:metabolism*; D008297:Male; D011817:Rabbits; D013928:Thromboxane A2 / Q000097:blood*; D014280:Triglycerides / Q000097:blood; D015430:Weight Gain / Q000187:drug effects
D002791:Cholesterol, Dietary; D014280:Triglycerides; D013928:Thromboxane A2; D005340:Fibrinogen; D002784:Cholesterol; D011464:Epoprostenol; D004121:Dimethyl Sulfoxide