title: IgG antibody and delayed-type hypersensitivity responses in nude mice grafted with thymic epithelium.
abstract: This study compared the ability of foetal thymic epithelium depleted of lymphocytes and dendritic cells, by low temperature or deoxyguanosine (dGuo) treatment in organ culture, to reconstitute T-cell function in nude mice. It is shown that renal capsule grafts of either type could promote the development of functional T lymphocytes in the periphery, as judged by in vivo assays. Both syngeneic and allogeneic thymic epithelium endowed nude mice with the capacity to mount IgG antibody and delayed-type hypersensitivity (DTH) responses to the T-dependent antigen ovalbumin (OVA). Functional reconstitution was accompanied by the appearance of Thy-1-bearing cells in the spleens of thymic grafted nude mice. The results from allogeneically grafted recipients show that a substantial population of peripheral T cells was present that collaborated with B cells and other antigen-presenting cells (APC) which do not express major histocompatibility complex (MHC) molecules of the thymus donor haplotype.
authors.affiliation: Division of Immunology, Medical School, University of Newcastle upon Tyne, U.K.
authors.forename: R K
authors.initials: RK
authors.lastname: Jordan
authors.forename: C J
authors.initials: CJ
authors.lastname: Chapman
authors.forename: S M
authors.initials: SM
authors.lastname: McLachlan
authors.forename: D
authors.initials: D
authors.lastname: Milne
authors.forename: B E
authors.initials: BE
authors.lastname: Loveland
authors.forename: J H
authors.initials: JH
authors.lastname: Robinson
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002455:Cell Division / Q000276:immunology; D003208:Concanavalin A / Q000276:immunology; D004848:Epithelium / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D051379:Mice; D008819:Mice, Nude; D010047:Ovalbumin / Q000276:immunology; D013154:Spleen / Q000276:immunology; D018800:Thy-1 Antigens; D013950:Thymus Gland / Q000276:immunology* / Q000637:transplantation
chemical_list: D000954:Antigens, Surface; D007074:Immunoglobulin G; D018800:Thy-1 Antigens; D003208:Concanavalin A; D010047:Ovalbumin