Synthetic peptides including acidic clusters as substrates of yeast casein kinase-2.
The synthesis is reported of a series of glutamyl peptide analogs of the model substrate H-Ser-Glu-Glu-Glu-Glu-Glu-OH of casein kinase-2 (CK-2). A convenient HPLC method for the separation of slightly different acidic peptides is also reported. The site specificity of yeast casein kinase-2 (Y-CK2) is examined with the aid of synthesized peptide substrates.
Marin
O
O
Biopolymer Research Centre, C.N.R., Department of Organic Chemistry, University of Padova, Italy.
Calderan
A
A
Ruzza
P
P
Borin
G
G
Meggio
F
F
Grankowski
N
N
Marchiori
F
F
D000595:Amino Acid Sequence; D047388:Casein Kinases; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005971:Glutamates; D018698:Glutamic Acid; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000302:isolation & purification / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013379:Substrate Specificity
D005971:Glutamates; D007202:Indicators and Reagents; D009842:Oligopeptides; D018698:Glutamic Acid; D011494:Protein Kinases; D047388:Casein Kinases