Ultrastructural demonstration of synaptic connections between calcitonin gene-related peptide immunoreactive axons and dynorphin A(1-8) immunoreactive dorsal horn neurons in a rat model of peripheral inflammation and hyperalgesia.
Synaptic contact between dynorphin A(1-8)-like immunoreactive lamina V spinal neurons and calcitonin gene-related peptide-like immunoreactive axon terminals was demonstrated using the immuno-electron microscopic mirror technique in a rat model of peripheral inflammation and hyperalgesia. Adjacent tissue sections were immunocytochemically labeled for either dynorphin A(1-8) or calcitonin gene-related peptide and examined at the electron microscopic level for the presence of synaptic contacts. The results suggest that some opioid neurons which exhibit a dynamic increase in dynorphin peptide associated with peripheral inflammation and hyperalgesia receive direct monosynaptic input from presumptive nociceptive primary afferents.
Takahashi
O
O
Neurobiology and Anesthesiology Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892.
Shiosaka
S
S
Traub
R J
RJ
Ruda
M A
MA
D000818:Animals; D001369:Axons / Q000737:chemistry / Q000648:ultrastructure; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis*; D004195:Disease Models, Animal; D004399:Dynorphins / Q000032:analysis*; D006930:Hyperalgesia / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D007249:Inflammation / Q000378:metabolism* / Q000473:pathology; D008297:Male; D016253:Microscopy, Immunoelectron; D009474:Neurons / Q000737:chemistry* / Q000648:ultrastructure; D010446:Peptide Fragments / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000737:chemistry* / Q000166:cytology / Q000648:ultrastructure; D013569:Synapses / Q000648:ultrastructure
D010446:Peptide Fragments; D004399:Dynorphins; C026770:dynorphin (1-8); D015740:Calcitonin Gene-Related Peptide