Influence of Ca/Na exchange and diamide on membrane partitioning of glyceraldehyde-3-phosphate dehydrogenase in dog red cells.
1. Ca/Na exchange in dog red cells is greatly stimulated by pretreatment of the cells with diamide, but only if the diamide preincubation is carried out in a Na-free, Ca-containing medium. 2. Membranes prepared from dog red cells that had been pre-exposed to diamide in solutions containing various combinations of Ca and Na were subjected to polyacrylamide gel electrophoresis and Western blotting. 3. The band representing glyceraldehyde-3-phosphate dehydrogenase was selectively increased under the very same ionic conditions that result in a diamide-induced stimulation of Ca/Na exchange. 4. Glyceraldehyde-3-phosphate dehydrogenase may participate in the modulation by diamide of Ca/Na exchange in dog red cells.
Colclasure
G C
GC
Department of Medicine, University of North Carolina, Chapel Hill 27599-7035.
Parker
J C
JC
D000818:Animals; D001391:Azo Compounds / Q000494:pharmacology*; D002118:Calcium / Q000097:blood*; D003958:Diamide / Q000494:pharmacology*; D004285:Dogs / Q000097:blood*; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000201:enzymology*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000097:blood*; D012964:Sodium / Q000097:blood*
D001391:Azo Compounds; D003958:Diamide; D012964:Sodium; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D002118:Calcium