title: Vaults. III. Vault ribonucleoprotein particles open into flower-like structures with octagonal symmetry.
abstract: The structure of rat liver vault ribonucleoprotein particles was examined using several different staining techniques in conjunction with EM and digestion with hydrolytic enzymes. Quantitative scanning transmission EM demonstrates that each vault particle has a total mass of 12.9 +/- 1 MD and contains two centers of mass, suggesting that each vault particle is a dimer. Freeze-etch reveals that each vault opens into delicate flower-like structures, in which eight rectangular petals are joined to a central ring, each by a thin hook. Vaults examined by negative stain and conventional transmission EM (CTEM) also reveal the flower-like structure. Trypsin treatment of vaults resulted exclusively in cleavage of the major vault protein (p104) and concurrently alters their structure as revealed by negative stain/CTEM, consistent with a localization of p104 to the flower petals. We propose a structural model that predicts the stoichiometry of vault proteins and RNA, defines vault dimer-monomer interactions, and describes two possible modes for unfolding of vaults into flowers. These highly dynamic structural variations are likely to play a role in vault function.
authors.affiliation: Department of Biological Chemistry, University of California, Los Angeles School of Medicine 90024-1737.
authors.forename: N L
authors.initials: NL
authors.lastname: Kedersha
authors.forename: J E
authors.initials: JE
authors.lastname: Heuser
authors.forename: D C
authors.initials: DC
authors.lastname: Chugani
authors.forename: L H
authors.initials: LH
authors.lastname: Rome
mesh_terms: D000818:Animals; D005613:Freeze Etching; D008099:Liver / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008961:Models, Structural; D011073:Polyamines; D000071228:Polyelectrolytes; D011108:Polymers / Q000378:metabolism; D051381:Rats; D012259:Ribonuclease, Pancreatic / Q000378:metabolism; D012261:Ribonucleoproteins / Q000378:metabolism / Q000648:ultrastructure*; D014357:Trypsin / Q000378:metabolism
chemical_list: D011073:Polyamines; D000071228:Polyelectrolytes; D011108:Polymers; D012259:Ribonuclease, Pancreatic; D012261:Ribonucleoproteins; D014357:Trypsin