Characterization of a new strain of HHV-6 (HHV-6SF) recovered from the saliva of an HIV-infected individual.
An isolate of the human herpesvirus-6 (HHV-6SF) recovered from the saliva of an HIV-infected individual differs in its cellular host range and certain genomic properties from other HHV-6 strains described. HHV-6SF replicates in adult peripheral blood mononuclear cells (PMC) substantially better than in fetal cord blood PMC and can be grown only in the MT-4 established T cell line. It preferentially infects CD4+ lymphocytes but can replicate in CD8+ cells and peripheral blood macrophages. It also infects neuroblastoma cells and cell lines derived from the gastrointestinal tract. These latter results suggest that this herpesvirus could play a role in disorders affecting these tissues. Finally, the restriction enzyme pattern of HHV-6SF differs from that of other HHV-6 strains. The identification of this distinct HHV-6 strain could indicate an unusual biologic variation among viral isolates thus far not observed with other herpesviruses.
Levy
J A
JA
Cancer Research Institute, University of California, School of Medicine, San Francisco 94143-0128.
Ferro
F
F
Lennette
E T
ET
Oshiro
L
L
Poulin
L
L
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications / Q000382:microbiology; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000382:microbiology / Q000648:ultrastructure; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D005767:Gastrointestinal Diseases / Q000150:complications / Q000382:microbiology; D006679:HIV Seropositivity / Q000150:complications / Q000382:microbiology; D006566:Herpesviridae Infections / Q000150:complications; D015654:Herpesvirus 6, Human / Q000145:classification* / Q000648:ultrastructure; D006801:Humans; D009447:Neuroblastoma / Q000209:etiology / Q000382:microbiology; D012463:Saliva / Q000382:microbiology*; D050378:T-Lymphocytes, Regulatory / Q000382:microbiology / Q000648:ultrastructure; D014779:Virus Replication
D000956:Antigens, Viral