title: Crystal structure of interleukin 8: symbiosis of NMR and crystallography.
abstract: The crystal structure of a host defense system chemotactic factor, interleukin 8, has been solved by molecular replacement using as a model the solution structure derived from nuclear magnetic resonance experiments. The structure was refined with 2 A x-ray data to an R factor of 0.187 (0.217 at 1.6 A). A comparison indicates some potential differences between the structure in solution and in the crystalline state. Our analysis also predicts that residues 4 through 9 on the amino terminus and the beta-bend, which includes His-33, may be important for receptor binding.
authors.affiliation: Crystallography Laboratory, National Cancer Institute-Frederick Cancer Research and Development Center, MD 21702.
authors.forename: E T
authors.initials: ET
authors.lastname: Baldwin
authors.forename: I T
authors.initials: IT
authors.lastname: Weber
authors.forename: R
authors.initials: R
authors.lastname: St Charles
authors.forename: J C
authors.initials: JC
authors.lastname: Xuan
authors.forename: E
authors.initials: E
authors.lastname: Appella
authors.forename: M
authors.initials: M
authors.lastname: Yamada
authors.forename: K
authors.initials: K
authors.lastname: Matsushima
authors.forename: B F
authors.initials: BF
authors.lastname: Edwards
authors.forename: G M
authors.initials: GM
authors.lastname: Clore
authors.forename: A M
authors.initials: AM
authors.lastname: Gronenborn
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D006860:Hydrogen Bonding; D016209:Interleukin-8 / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D014961:X-Ray Diffraction
chemical_list: D016209:Interleukin-8; D011994:Recombinant Proteins