title: Evidence for the presence of androgen receptors in human Leydig cells.
abstract: Localization of androgen receptors (ARs) in the human testis Leydig cells was examined with an AR assay and Northern blot analysis. Leydig cells, highly purified on a Percoll gradient, were used for the experiments. AR concentration in the total cell extract containing both the cytosol and nuclear fractions in Leydig cells was measured using [3H]methyltrienolone. ARs in Leydig cells showed a high affinity for [3H]methyltrienolone and the Kd and Bmax of the receptors were 1.24 nM and 11.7 fmol/mg protein, respectively. Northern blot analysis, using a 32P-labeled full-length human AR complementary DNA (cDNA) detected a 9.5-kb hybridizing band in the total RNA extracted from Leydig cells. These data can be interpreted as evidence of the existence of ARs in human Leydig cells.
authors.affiliation: Department of Urology, Osaka University Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Namiki
authors.forename: K
authors.initials: K
authors.lastname: Yokokawa
authors.forename: A
authors.initials: A
authors.lastname: Okuyama
authors.forename: E
authors.initials: E
authors.lastname: Koh
authors.forename: H
authors.initials: H
authors.lastname: Kiyohara
authors.forename: M
authors.initials: M
authors.lastname: Nakao
authors.forename: S
authors.initials: S
authors.lastname: Sakoda
authors.forename: K
authors.initials: K
authors.lastname: Matsumoto
authors.forename: T
authors.initials: T
authors.lastname: Sonoda
mesh_terms: D015152:Blotting, Northern; D006801:Humans; D066298:In Vitro Techniques; D007985:Leydig Cells / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism*; D013737:Testis / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D011944:Receptors, Androgen