Comparison between cobalt and pyrazole in the increased expression of coumarin 7-hydroxylase in mouse liver.
The data in this report show that administration of both cobalt and pyrazole results in an elevation in the amount of hepatic mRNA encoding for microsomal P45015 alpha/P450Coh, an increase in the amount of P450Coh protein, and an activation of COH and to a lesser extent testosterone 15 alpha-hydroxylase in two inbred strains of mice. Considerable quantitative differences between the two compounds and the two mouse strains in the response suggest that the effects of cobalt and pyrazole are mediated, at least partly, through different mechanisms. It is of interest that human hepatic COH resembles very closely that in the mouse liver.
Kocer
Z
Z
Department of Pharmacology and Toxicology, University of Oulu, Finland.
Raunio
H
H
Pasanen
M
M
Arvela
P
P
Raiskila
T
T
Honkakoski
P
P
Lang
M A
MA
Negishi
M
M
Pelkonen
O
O
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D015153:Blotting, Western; D003035:Cobalt / Q000494:pharmacology*; D065637:Cytochrome P-450 CYP2A6; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D015342:DNA Probes / Q000138:chemical synthesis; D004789:Enzyme Activation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism*; D011720:Pyrazoles / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis / Q000302:isolation & purification; D013250:Steroid Hydroxylases / Q000378:metabolism
D015342:DNA Probes; D011720:Pyrazoles; D012333:RNA, Messenger; D003035:Cobalt; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; D065637:Cytochrome P-450 CYP2A6; C050356:steroid 15-alpha-hydroxylase