Use of the fish enzyme system in monitoring water quality: effects of mercury on tissue enzymes.
1. Rosy barb (Puntius conchonius) were exposed to 181 micrograms/l mercuric chloride for 48 h and the activity of acid and alkaline phosphatases (AcP and AIP), aspartate aminotransferase (AAT), alanine aminotransferase (AIAT), lactic dehydrogenase (LDH), and acetylcholinesterase (AchE) were measured in vivo in several organs. 2. The AcP activity was inhibited in the liver, gills, kidneys, and gut but stimulated in the gonads. With the exception of kidney, the AIP activity showed an increase in all the organs examined. The AAT and AIAT were generally inhibited in different organs. An increase in LDH activity occurred in the cardiac and skeletal muscles while the AchE activity was considerably lowered in the brain, gills, and liver. 3. In vitro exposure to mercury at concentrations ranging between 10(-10) and 10(-4) M, inhibited the AIP, AAT, AIAT, LDH, and AchE activities in the tissues examined. The AcP activity was also depressed in all the tissues except in the testes, in which a marginal increase was noted. 4. The in vivo and in vitro effects of Hg were not of similar quality implying sequestration of toxic cations in the intact animals.
Gill
T S
TS
Department of Anatomy, Daniel Baugh Institute of Anatomy, Thomas Jefferson University, Philadelphia, PA 19107.
Tewari
H
H
Pande
J
J
D000110:Acetylcholinesterase / Q000378:metabolism; D000135:Acid Phosphatase / Q000037:antagonists & inhibitors / Q000378:metabolism; D000410:Alanine Transaminase / Q000037:antagonists & inhibitors / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000037:antagonists & inhibitors / Q000378:metabolism; D002800:Cholinesterase Inhibitors; D005260:Female; D005399:Fishes / Q000378:metabolism*; D005880:Gills / Q000201:enzymology; D007422:Intestines / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008297:Male; D008627:Mercuric Chloride / Q000008:administration & dosage / Q000633:toxicity*; D009132:Muscles / Q000201:enzymology; D010053:Ovary / Q000201:enzymology; D013737:Testis / Q000201:enzymology; D014873:Water Pollutants / Q000633:toxicity*
D002800:Cholinesterase Inhibitors; D014873:Water Pollutants; D008627:Mercuric Chloride; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000110:Acetylcholinesterase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase