A guanine nucleotide-binding protein in human astrocytoma.
We compared, by immunoblotting and immunohistochemical staining, the distribution of the alpha subunit of Go in human normal brain tissue with that in human brain tumours of neuroectodermal origin. The ten tumour samples included seven high-grade astrocytomas (grades III and IV) and three low-grade astrocytomas (grade II). Immunoreactivity of high-grade astrocytomas with the alpha subunit of Go (Go alpha) antibody was lower than that of low-grade astrocytomas and normal white matter. In the immunohistochemical study, neoplastic cells in the astrocytomas were found to contain little or no significant Go alpha, although nonlesional white matter was strongly stained with Go alpha antibody. This study suggests that Go alpha antibody may assist in defining the boundary of an astrocytoma, and the difference in Go content may affect the alteration in the physical properties of the membrane, which exhibit, in part, the character of the transformation.
Takenaka
K
K
Department of Neurosurgery, Gifu University School of Medicine, Japan.
Kanaho
Y
Y
Hara
A
A
Zhang
W
W
Ando
T
T
Sakai
N
N
Yamada
H
H
Nozawa
Y
Y
D001254:Astrocytoma / Q000737:chemistry*; D001932:Brain Neoplasms / Q000737:chemistry*; D019204:GTP-Binding Proteins / Q000032:analysis*; D005909:Glioblastoma / Q000737:chemistry*; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D008565:Membrane Proteins / Q000032:analysis; D009363:Neoplasm Proteins / Q000032:analysis*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009474:Neurons / Q000737:chemistry
D008565:Membrane Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D019204:GTP-Binding Proteins