title: Mapping the gene encoding the human erythroid transcriptional factor NFE1-GF1 to Xp11.23.
abstract: The X-linked NFE1 gene encodes an erythroid factor involved in globin gene transcription. Using a human cDNA clone encoding this factor, we show, by in situ hybridization and by analysis of human-rodent hybrid cell lines, that this gene is located in Xp11.23. In the absence of polymorphisms in the NFE1 gene, these results allow the study of the possible relationships between NFE1 mutations and X-linked hereditary persistence of fetal hemoglobin by linkage analysis with RFLP markers of the region. A female patient, hemizygous for the NFE1 locus, shows essentially normal hematological parameters.
authors.affiliation: Biologia Generale e Genetica Medica, Università di Pavia, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Caiulo
authors.forename: S
authors.initials: S
authors.lastname: Nicolis
authors.forename: P
authors.initials: P
authors.lastname: Bianchi
authors.forename: O
authors.initials: O
authors.lastname: Zuffardi
authors.forename: B
authors.initials: B
authors.lastname: Bardoni
authors.forename: P
authors.initials: P
authors.lastname: Maraschio
authors.forename: S
authors.initials: S
authors.lastname: Ottolenghi
authors.forename: G
authors.initials: G
authors.lastname: Camerino
authors.forename: B
authors.initials: B
authors.lastname: Giglioni
mesh_terms: D002460:Cell Line; D002648:Child; D002871:Chromosome Banding; D002874:Chromosome Mapping; D015342:DNA Probes; D004268:DNA-Binding Proteins / Q000235:genetics*; D050981:Erythroid-Specific DNA-Binding Factors; D005260:Female; D015723:Gene Library; D006801:Humans; D007621:Karyotyping; D008297:Male; D015183:Restriction Mapping; D014157:Transcription Factors / Q000235:genetics*; D014960:X Chromosome
chemical_list: D015342:DNA Probes; D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D014157:Transcription Factors