A novel 1745-dalton pyroglutamyl peptide derived from chromogranin B is in the bovine adrenomedullary chromaffin vesicle.
1. Following the recent demonstration of a glutaminyl cyclase activity localized in adrenomedullary chromaffin vesicles, an assay was developed to isolate and characterize posttranslationally modified peptides from this tissue which contain pyroglutamate. This assay consisted of spectrometric identification of peptides before and after enzymatic removal of pyroglutamyl residues. 2. Using this procedure, a pyroglutamyl peptide (BAM-1745) was isolated and sequenced and was shown to be a significant component of adrenomedullary secretory vesicles. 3. A computer search through the Swiss-Prot protein sequence database revealed a 93% identity of BAM-1745 and a fragment of human chromogranin B (Gln580-Tyr593).
Flanagan
T
T
Division of Medicinal Biochemistry, Wellcome Research Laboratories, Research Triangle Park, North Carolina 27709.
Taylor
L
L
Poulter
L
L
Viveros
O H
OH
Diliberto
E J
EJ
D000217:Acyltransferases / Q000032:analysis*; D000313:Adrenal Medulla / Q000737:chemistry*; D000595:Amino Acid Sequence; D019881:Aminoacyltransferases; D000818:Animals; D002417:Cattle; D002837:Chromaffin Granules / Q000737:chemistry*; D053378:Chromogranin B; D002864:Chromogranins / Q000032:analysis* / Q000138:chemical synthesis; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis* / Q000138:chemical synthesis; D011499:Protein Processing, Post-Translational; D011751:Pyroglutamyl-Peptidase I; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D012689:Sequence Homology, Nucleic Acid; D012697:Serine Endopeptidases; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods
C506134:CHGB protein, human; D053378:Chromogranin B; D002864:Chromogranins; D010446:Peptide Fragments; C068759:BAM 1745; D000217:Acyltransferases; D019881:Aminoacyltransferases; C059280:glutaminyl-peptide cyclotransferase; D011751:Pyroglutamyl-Peptidase I; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D011761:Pyrrolidonecarboxylic Acid