title: Demonstration of a cell wall antigen cross-reacting with cryptococcal polysaccharide in experimental disseminated trichosporonosis.
abstract: Patients with disseminated infections caused by Trichosporon beigelii have a circulating antigen that cross-reacts with the polysaccharide capsule of Cryptococcus neoformans. We studied the localization of this antigen by immunoelectron microscopy in a rabbit model of experimental disseminated trichosporonosis. Deparaffinized lung sections were examined by using a murine monoclonal anti-cryptococcal polysaccharide antibody and colloidal gold particles coated with goat antibody to murine immunoglobulin G. Antigen that cross-reacted with the monoclonal antibody was observed in the T. beigelii cell wall and in a fibrillar matrix extending from the cell wall.
authors.affiliation: Department of Medicine, Wilford Hall U.S. Air Force Medical Center, Lackland Air Force Base, Texas 78236.
authors.forename: G P
authors.initials: GP
authors.lastname: Melcher
authors.forename: K D
authors.initials: KD
authors.lastname: Reed
authors.forename: M G
authors.initials: MG
authors.lastname: Rinaldi
authors.forename: J W
authors.initials: JW
authors.lastname: Lee
authors.forename: P A
authors.initials: PA
authors.lastname: Pizzo
authors.forename: T J
authors.initials: TJ
authors.lastname: Walsh
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000946:Antigens, Fungal / Q000032:analysis*; D002473:Cell Wall / Q000276:immunology* / Q000648:ultrastructure; D003429:Cross Reactions; D003455:Cryptococcus neoformans / Q000276:immunology*; D004195:Disease Models, Animal; D007074:Immunoglobulin G / Q000276:immunology; D016253:Microscopy, Immunoelectron; D009181:Mycoses / Q000175:diagnosis* / Q000473:pathology; D011134:Polysaccharides / Q000276:immunology*; D011817:Rabbits; D014250:Trichosporon / Q000276:immunology* / Q000648:ultrastructure
chemical_list: D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal; D007074:Immunoglobulin G; D011134:Polysaccharides; C014426:cryptococcal polysaccharide