title: Distribution of myosin mRNA during development and regeneration of skeletal muscle fibers.
abstract: Myosin mRNA distribution among subcellular compartments of anterior tibialis muscles in rabbit is monitored by in situ hybridization. A high density of mRNA was widely distributed throughout myotubes from 29-day fetal muscle and from regenerating adult muscle. All cytoplasmic spaces contained mRNA except where scattered myofibrils and centrally located nuclei were found. In fibers from 22-week-old rabbits, myosin mRNA was concentrated under the sarcolemma and excluded from the consolidated myofibrils and peripheral nuclei. The dispersal of mRNA through the cytoplasm in myotubes suggests that translation of myosin is widespread and that rapid myofibril assembly can occur throughout the fiber.
authors.affiliation: Department of Physiology and Biophysics, University of Illinois, Chicago 60680.
authors.forename: D J
authors.initials: DJ
authors.lastname: Dix
authors.forename: B R
authors.initials: BR
authors.lastname: Eisenberg
mesh_terms: D000818:Animals; D002451:Cell Compartmentation; D024510:Muscle Development; D009132:Muscles / Q000196:embryology / Q000502:physiology*; D009218:Myosins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits / Q000196:embryology; D012038:Regeneration
chemical_list: D012333:RNA, Messenger; D009218:Myosins