Amplification of oncogenes in mammary carcinoma shown by fine-needle biopsy.
A procedure that measures the amplification of oncogenes in human cancer cells is described. The cells were obtained by fine-needle biopsy to allow repeated sampling from individual metastases. A drawback was the low number of cells obtained, but this could be overcome by using a slot-blot hybridization technique to measure gene amplification. Two patients with mammary cancer (primary tumors or metastases), analyzed for the levels of amplification of the oncogene erb-B2, are described in detail. This technique is suitable for analyzing alterations occurring during cancer progression and for identifying subgroups of mammary cancer with different characteristics.
Lönn
U
U
Radiumhemmet, Karolinska Hospital, Stockholm, Sweden.
Lönn
S
S
Nylen
U
U
Winblad
G
G
Stenkvist
B
B
D000368:Aged; D001707:Biopsy, Needle / Q000379:methods; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005784:Gene Amplification; D006801:Humans; D008207:Lymphatic Metastasis; D008875:Middle Aged; D009857:Oncogenes / Q000235:genetics*
D004273:DNA, Neoplasm