Hepatic eosinophilopoiesis from multipotent hemopoietic stem cells in Toxocara canis-infected mice.
Extramedullary hemopoiesis, recognized as hemopoietic foci, increased in the livers of Toxocara canis-infected mice. At the peak of the response (day-13 after infection), the majority of hepatic hemopoietic foci were of the eosinophil lineage. Hepatic nonparenchymal cells prepared from T. canis-infected mice on day 13 contained large numbers of hemopoietic stem cells, more than half of which were cycling. When W/Wv mice, which are genetically deficient in multipotent hemopoietic stem cells, were infected with T. canis, hepatic hemopoietic foci were rare throughout the course of infection. This impaired response of W/Wv mice was restored by bone marrow grafting from normal +/+ littermates. These results indicate that, in response to the increased demand, eosinophils are generated in the liver by the differentiation from multipotent stem cells, not only from the committed precursors.
Maruyama
H
H
Department of Parasitology, Miyazaki Medical College, Japan.
Higa
A
A
Asami
M
M
Owhashi
M
M
Nawa
Y
Y
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D004804:Eosinophils / Q000166:cytology* / Q000469:parasitology / Q000502:physiology; D005260:Female; D006410:Hematopoiesis / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000469:parasitology / Q000502:physiology; D008099:Liver / Q000166:cytology* / Q000469:parasitology / Q000502:physiology; D051379:Mice; D014119:Toxocara / Q000302:isolation & purification / Q000502:physiology; D014120:Toxocariasis / Q000503:physiopathology*