Constitutive expression of a groEL-related protein on the surface of human gamma/delta cells.
Rabbit antibodies to hsp58 (P1), the human homologue of the Escherichia coli stress protein groEL, react specifically in indirect immunofluorescence and complement-dependent microcytoxicity experiments with a cell surface antigen expressed constitutively by T cell lines bearing gamma/delta receptors. This anti-hsp58-reactive antigen is not demonstrable on T cells that express alpha/beta receptors or on various cells that lack T cell receptors. Certain evidence was obtained to suggest that the target antigen on the surface of gamma/delta T cells is a approximately 77-kD protein distinct from intracellular hsp58 and known members of the hsp70 stress protein family. While the exact nature and significance of this anti-hsp58-reactive protein remain to be determined, these data may help to clarify the roles of groEL-related stress proteins and gamma/delta cells that recognize groEL homologous in immunologic defense against infection and in autoimmune disease.
Jarjour
W
W
Division of Rheumatology and Immunology, University of North Carolina, Chapel Hill 27514.
Mizzen
L A
LA
Welch
W J
WJ
Denning
S
S
Shaw
M
M
Mimura
T
T
Haynes
B F
BF
Winfield
J B
JB
D000906:Antibodies; D000954:Antigens, Surface / Q000032:analysis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D002460:Cell Line; D002478:Cells, Cultured; D018834:Chaperonin 60; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005455:Fluorescent Antibody Technique; D006360:Heat-Shock Proteins / Q000032:analysis / Q000235:genetics*; D006801:Humans; D015151:Immunoblotting; D008970:Molecular Weight; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology*
D000906:Antibodies; D000954:Antigens, Surface; D001426:Bacterial Proteins; D018834:Chaperonin 60; D006360:Heat-Shock Proteins; D011948:Receptors, Antigen, T-Cell