title: Host range and symptoms are determined by specific domains of the maize streak virus genome.
abstract: We have cloned two distinct symptomatic variants of the geminivirus streak virus from maize plants infected with the Nigerian strain (MSV-N). Following "agroinoculation" to maize plants MSV-Nm produces narrow, mildly chlorotic discontinuous streaks, whereas MSV-Ns-infected tissue has wide, severely chlorotic streaks. Symptom appearance is delayed following MSV-Nm inoculation. MSV-Nm has a narrow host range within the Gramineae comprising a fraction of that of the wide host range isolate MSV-Ns. The two isolates are highly homologous and have identical restriction enzyme maps. In order to localize the determinants of pathogenicity we constructed, in vitro, hybrid genomes by restriction enzyme fragment exchange. The determinants of host range, severity of chlorosis, streak length, and timing of symptom appearance map to a fragment which includes the large intergenic region and the 5' terminus of the complementary sense C1 gene. Streak width is determined by the virion-sense portion of the genome, which is consistent with the observation that the virion-sense gene products (V1 and V2) are required for spread of the virus.
authors.affiliation: John Innes Centre for Plant Science Research, Norwich, United Kingdom.
authors.forename: M I
authors.initials: MI
authors.lastname: Boulton
authors.forename: D I
authors.initials: DI
authors.lastname: King
authors.forename: P G
authors.initials: PG
authors.lastname: Markham
authors.forename: M S
authors.initials: MS
authors.lastname: Pinner
authors.forename: J W
authors.initials: JW
authors.lastname: Davies
mesh_terms: D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005814:Genes, Viral; D010942:Plant Viruses / Q000235:genetics* / Q000472:pathogenicity; D015183:Restriction Mapping; D014774:Virulence / Q000235:genetics; D003313:Zea mays
chemical_list: D004279:DNA, Viral