Vaccinia virus preferentially enters polarized epithelial cells through the basolateral surface.
The uptake of vaccinia virus in polarized epithelial cells was studied to determine whether the site of entry was confined to either the apical or the basolateral membrane. Virus infection was monitored with a recombinant vaccinia virus carrying the luciferase reporter gene. Using cell lines MDCK and MDCK-D11, a clonal line with high transepithelial electrical resistance, we determined that vaccinia virus preferentially enters through the basolateral membrane. The possibility that there is a polarized cell surface distribution of vaccinia virus receptors which may be involved in systemic poxvirus infections is discussed.
Rodriguez
D
D
Department of Biochemistry, State University of New York, Health Science Center, Brooklyn 11203.
Rodriguez
J R
JR
Ojakian
G K
GK
Esteban
M
M
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology*; D004848:Epithelium / Q000502:physiology; D007365:Intercellular Junctions / Q000502:physiology; D007700:Kinetics; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D011991:Receptors, Virus / Q000502:physiology; D013997:Time Factors; D014616:Vaccinia virus / Q000235:genetics / Q000502:physiology*
D011991:Receptors, Virus; D008156:Luciferases