The role of monocytes in IL-3 enhancement of human T cell proliferation.
We have assessed the role of recombinant human interleukin 3 (rIL-3) in the activation and proliferation of peripheral blood lymphocytes (PBL) and their various subsets. rIL-3 significantly enhanced the CD2-responsiveness of unfractionated PBL. Proliferating cells were mainly T lymphocytes and most of them expressed CD4. This effect was absent following adherent cell depletion from PBL. rIL-3 also failed to directly activate purified lymphocytes, nor did it increase T cell responsiveness to mitogens and/or IL-2. By contrast, rIL-3 induced monocyte functions as enhancement of IL-1 production was observed following treatment of these cells by rIL-3. This effect was removed by anti IL-3 mAb addition to these cultures. In addition, anti IL-1 antibody partially inhibits IL-3-derived responses. Together, these data strongly suggest that IL-3 stimulation of T lymphocytes is related mainly to its ability to enhance monocyte functions.
Mossalayi
M D
MD
Laboratoire d'Immunologie Cellulaire, CNRS URA186, Hôpital de La Pitié-Salpetrière, Paris, France.
Dalloul
A H
AH
Bertho
J M
JM
Lecron
J C
JC
Debré
P
P
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000037:antagonists & inhibitors; D018801:CD2 Antigens; D002448:Cell Adhesion; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000096:biosynthesis; D007376:Interleukin-2 / Q000494:pharmacology; D007377:Interleukin-3 / Q000037:antagonists & inhibitors / Q000276:immunology / Q000494:pharmacology*; D008213:Lymphocyte Activation; D009000:Monocytes / Q000166:cytology / Q000276:immunology*; D011971:Receptors, Immunologic / Q000037:antagonists & inhibitors; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D007375:Interleukin-1; D007376:Interleukin-2; D007377:Interleukin-3; D011971:Receptors, Immunologic