title: A method for immunocytochemical identification of biocytin-labeled neurons following intracellular recording.
abstract: Because of the large number of cell phenotypes in the nervous system, it has been difficult to characterize each as to specific electrophysiological properties. We have developed a technique that allows the identification of central and peripheral nervous system neurons following intracellular recording. We use electrodes that contain 2% biocytin to do current- and voltage-clamp recordings; the recorded neurons are revealed with streptavidin-fluorescein isothiocyanate labeling and identified through immunohistochemical staining for specific antigens. Presently, we report on the use of this technique to identify four cell types--dopamine, beta-endorphin, vasopressin and oxytocin--in the hypothalamus of the mammal. This technique should have widespread applicability for electrophysiologists.
authors.affiliation: Department of Physiology, Oregon Health Sciences University, Portland 97201.
authors.forename: O K
authors.initials: OK
authors.lastname: Rønnekleiv
authors.forename: M D
authors.initials: MD
authors.lastname: Loose
authors.forename: K R
authors.initials: KR
authors.lastname: Erickson
authors.forename: M J
authors.initials: MJ
authors.lastname: Kelly
mesh_terms: D000818:Animals; D001127:Arginine Vasopressin / Q000032:analysis; D004594:Electrophysiology; D005260:Female; D006168:Guinea Pigs; D007031:Hypothalamus / Q000166:cytology; D007150:Immunohistochemistry / Q000379:methods*; D008239:Lysine / Q000031:analogs & derivatives*; D008297:Male; D008839:Microelectrodes; D009474:Neurons / Q000737:chemistry* / Q000502:physiology; D010121:Oxytocin / Q000032:analysis; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis; D001615:beta-Endorphin / Q000032:analysis
chemical_list: D001127:Arginine Vasopressin; D010121:Oxytocin; D001615:beta-Endorphin; D014446:Tyrosine 3-Monooxygenase; C013411:biocytin; D008239:Lysine