Isolation of novel complementary DNA clones from T lymphoma cells: one encodes a putative multiple membrane-spanning protein.
Five novel complementary DNA (cDNA) clones which are differentially expressed between two closely related T lymphoma cell clones were isolated using subtraction-enriched differential screening. SL12.4 cells, from which the cDNAs were isolated, have characteristics of thymocytes at an intermediate stage in development and cause prominent extranodal ovarian tumors in syngeneic animals. A sister cell clone, SL12.3, derived from the same tumor, has a distinct phenotype and causes more aggressive, diffuse lymphomas. Four of the five novel genes are expressed in normal thymus, activated spleen cells, or gut-associated lymphoid tissue. The DNA sequence and predicted protein sequence are presented for one of the novel cDNA clones. This novel cDNA clone detects mRNA in normal thymus, gut-associated lymphoid tissue, and ovarian tissue. The predicted protein has four putative transmembrane-spanning regions. The expression of the transcript is repressed in somatic cell hybrids formed from SL12.4 cells fused with three different T lymphoma cell lines which lack detectable mRNA complementary to the novel cDNA clone. This trans-negative regulation suggests that the expression of the gene is regulated by repressional mechanisms.
MacLeod
C L
CL
Department of Medicine, University of California, San Diego, La Jolla 92093.
Fong
A M
AM
Seal
B S
BS
Walls
L
L
Wilkinson
M F
MF
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000302:isolation & purification*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D006822:Hybrid Cells / Q000737:chemistry; D008221:Lymphoid Tissue / Q000737:chemistry; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000473:pathology*; D008322:Mammals / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000235:genetics; D010053:Ovary / Q000737:chemistry; D010641:Phenotype; D011061:Poly A / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D013045:Species Specificity; D013154:Spleen / Q000737:chemistry; D013950:Thymus Gland / Q000737:chemistry; D014407:Tumor Cells, Cultured / Q000737:chemistry
D008565:Membrane Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011061:Poly A; D004247:DNA