title: Processing and intracellular sorting of anglerfish and rat preprosomatostatins in mammalian endocrine cells.
abstract: Rat preprosomatostatin (rPPSS) is processed to two distinct end products in a tissue-specific manner. The analogous end products in anglerfish are derived from separate precursors, anglerfish preprosomatostatins-1 and -2 (a(1)PPSS and a(II)PPSS). This report reviews experiments demonstrating that in mammalian cells, the cell of expression, not precursor structure, determines the processing fate of the preprosomatostatins. A fusion precursor of a(II)PPSS and rPPSS was expressed in mammalian cell lines to determine that the amino-terminal 78 residues of rPPSS contain a sorting signal that directs the precursor into a regulated secretory pathway wherein proteolytic processing occurs. Preliminary studies of rPPSS pro-region mutations are presented that attempt to further localize this sorting signal.
authors.affiliation: Department of Psychiatry, Yale University School of Medicine, New Haven, CT 06508.
authors.forename: K A
authors.initials: KA
authors.lastname: Sevarino
authors.forename: R
authors.initials: R
authors.lastname: Ventimiglia
authors.forename: P
authors.initials: P
authors.lastname: Stork
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D005399:Fishes; D009154:Mutation; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D013004:Somatostatin / Q000235:genetics* / Q000378:metabolism; D013045:Species Specificity
chemical_list: D011498:Protein Precursors; D013004:Somatostatin