Generation and characterization of an HLA-DR alpha-specific monoclonal antibody using L-cell transfectants expressing human and mouse class II major histocompatibility dimers.
Among the HLA-DR-specific monoclonal antibodies (mAbs) that have been characterized previously, there is a marked shortage of DR alpha chain-specific mAbs which stain unfixed cells. This may result from the high degree of sequence similarity between the alpha 1 domains of HLA-DR and H-2E leading to a state of cross-tolerance to DR alpha in H-2-expressing mice. BALB/b (E-negative) mice were immunized with DR1-transfected mouse L cells. The chain specificity of the resulting DR-specific mAb was determined using a panel of transfectants expressing hybrid mouse/human class II heterodimers. A DR alpha-specific mAb was generated which was capable of immunoprecipitating DR alpha beta dimers and inhibiting the anti-DR alloresponse of human T-cell clones. The present study demonstrates that, with the selection of a suitable recipient strain, transfectants can be useful in the generation and definition of chain-specific mouse mAbs.
Palmer
D
D
Department of Immunology, Royal Postgraduate Medical School, Hammersmith Hospital, England.
Kevany
M
M
Mackworth-Young
C
C
Batchelor
R
R
Lombardi
G
G
Lechler
R
R
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000918:Antibody Specificity; D006684:HLA-DR Antigens / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007114:Immunization; D007739:L Cells; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D011233:Precipitin Tests; D011994:Recombinant Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection
D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D011994:Recombinant Proteins