title: Normal phase HPLC as a model system of specific benzodiazepine-receptor binding.
abstract: The hypothesis that benzodiazepines interact with their receptors in the CNS via hydrogen bonding mediated by the carbonyl and imine groups has been studied by correlating a physicochemical parameter affected by hydrogen bonds potentially accepted or donated by benzodiazepines with their binding constants to synaptosomal rat brain membranes. The reference physicochemical system chosen was normal phase high-performance liquid chromatography on mu-Bondapack NH2 (propylamine groups chemically bonded to porous silica) eluted with mixtures of n-hexane and 2-propanol. A strong correlation has been found for binding of the implicated groups to the column and to the receptor.
authors.affiliation: Centro di Strutturistica Diffratometrica, Universitá di Ferrara, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Gilli
authors.forename: M C
authors.initials: MC
authors.lastname: Pietrogrande
authors.forename: P A
authors.initials: PA
authors.lastname: Borea
mesh_terms: D000433:1-Propanol; D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D006586:Hexanes; D006860:Hydrogen Bonding; D066298:In Vitro Techniques; D007700:Kinetics; D008956:Models, Chemical; D051381:Rats; D011963:Receptors, GABA-A / Q000737:chemistry / Q000378:metabolism*; D012822:Silicon Dioxide; D012997:Solvents; D013574:Synaptosomes / Q000378:metabolism
chemical_list: D006586:Hexanes; D011963:Receptors, GABA-A; D012997:Solvents; C026385:n-hexane; D012822:Silicon Dioxide; D000433:1-Propanol