title: Detection of a progesterone-induced secretory protein synthesized by the uteri but not the endometriotic implants of rats with induced endometriosis.
abstract: Steroids modulate the secretory activity of the uterus, but little is known of their effect on ectopic endometrium protein synthesis and secretion. We utilized two-dimensional electrophoresis to visualize proteins produced by the uteri and endometriotic implants of both steroid-treated and reproductively cyclic rats with and without surgically induced endometriosis. Of the greater than 300 proteins visualized, only the uterine cultures from progesterone (P)-stimulated, estrogen-suppressed rats contained a distinctive glycoprotein (P-induced uterine protein-1; molecular weight [Mr] 70,000; isoelectric point [pI] 5.7). This protein was not detected in any of the endometriotic implant cultures. Progesterone-induced uterine protein-1 could play a role in luteal or endometrial physiology and may be valuable in assessing endometrial function. The aberrant secretory behavior of the ectopic endometrium suggests a possible involvement in the reproductive dysfunction associated with endometriosis.
authors.affiliation: Department of Obstetrics and Gynecology, University of Kentucky, Lexington.
authors.forename: K L
authors.initials: KL
authors.lastname: Sharpe
authors.forename: M C
authors.initials: MC
authors.lastname: Bertero
authors.forename: M W
authors.initials: MW
authors.lastname: Vernon
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D004715:Endometriosis / Q000378:metabolism*; D004958:Estradiol / Q000097:blood; D004971:Estrus / Q000502:physiology*; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D007987:Gonadotropin-Releasing Hormone / Q000037:antagonists & inhibitors; D009842:Oligopeptides / Q000494:pharmacology*; D011374:Progesterone / Q000097:blood / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D014182:Transplantation, Autologous; D014599:Uterus / Q000187:drug effects / Q000378:metabolism* / Q000637:transplantation
chemical_list: D006023:Glycoproteins; D009842:Oligopeptides; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D004958:Estradiol; C057022:iturelix