Microparticle-enhanced nephelometric immunoassay (Nephelia) for immunoglobulins G, A, and M.
Covalent binding of gamma chains of IgG, whole IgA, and mu chains of IgM on polyfunctional hydrophilic microspheres (MS) yields MS-Ig conjugates, usable as reagents in new microparticle-enhanced nephelometric immunoassays (Nephelia). The principle of the assays is inhibition by free analyte (IgG, IgA, and IgM) of agglutination of the MS-Ig conjugate with specific antiserum, the light scattered by the aggregates being measured with a nephelometer. The immunoglobulin assays developed are easy to perform (single-step assays, no washing or phase separation) and sensitive (high dilution of biological samples to exclude interferences and pretreatment). Analytical recovery results (95.4-101.2%) and correlations with generally used commercial assays (r = 0.86-0.98) indicate that the assays are accurate for large concentration ranges of immunoglobulins. Precision study gives CVs = 2.8-9.6%. Nephelia appears to be useful for quantifying a large variety of biological molecules.
Cuilliere
M L
ML
Immunology Laboratory, Faculty of Medicine, Vandoeuvre-les-Nancy, France.
Montagne
P
P
Bessou
T
T
el Omari
R
R
Riochet
D
D
Varcin
P
P
Laroche
P
P
Prud'homme
P
P
Marchand
J
J
Flecheux
O
O
D006801:Humans; D007118:Immunoassay / Q000706:statistics & numerical data; D007070:Immunoglobulin A / Q000032:analysis* / Q000134:cerebrospinal fluid; D007074:Immunoglobulin G / Q000032:analysis* / Q000134:cerebrospinal fluid; D007075:Immunoglobulin M / Q000032:analysis* / Q000134:cerebrospinal fluid; D007700:Kinetics; D008863:Microspheres; D009391:Nephelometry and Turbidimetry; D011786:Quality Control
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M