Azido derivative of tricarboxylic acid for photoaffinity labeling.
A new photoaffinity probe, 5-(1-hydroxy-4-azidophenylazo)-1,2,3-benzenetricarboxylic acid, was synthesized and characterized. This reagent can be potentially used in photoaffinity labeling of the mitochondrial tricarboxylate carrier, as well as of enzymes interacting with tricarboxylic acids. Inhibition and labeling of the mitochondrial tricarboxylate carrier is presented.
Szewczyk
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A
Institut für Biochemie und Molekularbiologie, Universität Bern, Switzerland.
Claeys
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D
Mertens
W
W
Azzi
A
A
D000345:Affinity Labels / Q000737:chemistry; D000818:Animals; D001386:Azides; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism; D010777:Photochemistry; D051381:Rats; D013057:Spectrum Analysis; D014233:Tricarboxylic Acids / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism
D000345:Affinity Labels; D001386:Azides; D002352:Carrier Proteins; D014233:Tricarboxylic Acids; C020723:citrate-binding transport protein