Stimulation by NaCl, polylysine and heparin of two forms of spleen tyrosine protein kinase immunologically related with the protein expressed by lyn oncogene.
The previously isolated spleen tyrosine protein kinase, conventionally termed TPK-IIA, displaying activation by either positively or negatively charged polyelectrolytes has been further characterized. TPK-IIA is immunologically related with the tyrosine protein kinase encoded by the lyn gene, a member of src subfamily and is dramatically activated by very high NaCl concentration. The stimulatory effects of NaCl and polylysine, which are not additive, are accounted for by increased Vmax values, the Km being virtually unchanged, suggesting that both effectors probably interact with the same site(s). Stimulation of TPK-IIA by heparin appears to be partially additive to that promoted by NaCl and possibly occurring through a different mechanism. The NaCl activatory effect correlates with the electrolytic nature of synthetic peptides used as substrates, being much more consistent with neutral peptides as compared with acidic ones. Of the other three spleen tyrosine protein kinases, TPK-I shows similar biochemical and immunological features, suggestive of close relatedness with TPK-IIA, while TPK-IIB and TPK-III are neither related with the lyn protein nor with the products of three other oncogenes of the src subfamily, namely lck, hck and fyn.
Brunati
A M
AM
Dipartimento di Chimica Biologica CNR, Università di Padova, Italy.
Donella-Deana
A
A
Ralph
S
S
Marchiori
F
F
Borin
G
G
Fischer
S
S
Pinna
L A
LA
D000818:Animals; D003429:Cross Reactions; D006493:Heparin / Q000494:pharmacology; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D011107:Polylysine / Q000494:pharmacology; D011505:Protein-Tyrosine Kinases / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000276:immunology; D051076:Proto-Oncogene Proteins c-fyn; D051572:Proto-Oncogene Proteins c-hck; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology; D013154:Spleen / Q000201:enzymology*; D013379:Substrate Specificity; D019061:src-Family Kinases
D007527:Isoenzymes; D011518:Proto-Oncogene Proteins; D011107:Polylysine; D012965:Sodium Chloride; D006493:Heparin; D011505:Protein-Tyrosine Kinases; C494961:Fyn protein, rat; C496633:Hck protein, rat; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D051076:Proto-Oncogene Proteins c-fyn; D051572:Proto-Oncogene Proteins c-hck; C060902:lyn protein-tyrosine kinase; D019061:src-Family Kinases