[Laboratory study on the relationship between drinking water and hepatoma--quantitative evaluation using hepatic gamma-GT method].
One to two-month-old male Fischer 344 (F-344) rats were used in this investigation. The experiment was devised simulating the Two-stage Hepatocarcinogenesis Model described by Pitot. Quantitative stereological method was applied to evaluate the gamma-GT enzyme altered foci (EAF) in examined liver slides. The results showed that pond-ditch water had an enhancement effect on the expression of gamma-GT in the livers of male F-344 rats, where as well water had no such effect. When AFB1 was given alone to the male F-344 rats after partial hepatectomy, there was no effect on gamma-GT, if it was followed by pond-ditch water the rats would express high frequency of EAF. In this experiment the effect of organochlorinated pesticide DDT on hepatic gamma-GT was also studied. It showed a strong influence on the quantity and size of EAF.
Zhou
T L
TL
School of Public Health, Shanghai Medical University.
Yu
S Z
SZ
D016604:Aflatoxin B1; D000348:Aflatoxins; D000818:Animals; D003634:DDT; D007091:Image Processing, Computer-Assisted; D008099:Liver / Q000201:enzymology*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology / Q000209:etiology*; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D014881:Water Supply; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
D000348:Aflatoxins; D016604:Aflatoxin B1; D003634:DDT; D005723:gamma-Glutamyltransferase