Importance of ICAM-1 for accessory cell function of monocytic cells.
We investigated to what extent the adherence molecule ICAM-1 is important for the accessory cell function of cells of the monocyte lineage. A monoclonal antibody to ICAM-1, 7F7, inhibited the accessory cell function of human peripheral blood monocytes cultured in the presence of serum for 1-3 days. Monocyte, cultured for 4-7 days still expressed ICAM-1 but their accessory cell function was no longer inhibited by the antibody 7F7. Dendritiform accessory cells, obtained by culture of peripheral blood monocytes in serum free medium for 1-2 days also expressed ICAM-1 and their capacity to stimulate T-cells was also abrogated by antibody 7F7. These findings suggest that ICAM-1 on the surface of accessory cells of a certain differentiation stage seems to contribute to the capacity of these cells to stimulate T-cells but, that in some situations accessory cell function can occur normally without requiring ICAM-1 dependent cell to cell interactions.
Neumayer
H P
HP
Institut für Hygiene, Innsbruck, Austria.
Schultz
T F
TF
Peters
J H
JH
Dierich
M P
MP
D000938:Antigen-Presenting Cells / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000502:physiology*; D003713:Dendritic Cells / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1; D008213:Lymphocyte Activation; D009000:Monocytes / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D015815:Cell Adhesion Molecules; D018799:Intercellular Adhesion Molecule-1