title: Attaching and effacing lesions in vivo and adhesion to tissue culture cells of Vero-cytotoxin-producing Escherichia coli belonging to serogroups O5 and O103.
abstract: Certain isolates of Escherichia coli from humans and animals with enteric disease attach to enterocytes and cause 'attaching and effacing' (AE) lesions. E. coli strain S22-1, serotype O103:H2, isolated from a child with diarrhoea, contained two plasmids; one of these (pDEP12) hybridized with the CVD419 DNA probe derived from a plasmid found in E. coli O157:H7 and associated with expression of fimbriae and ability to adhere to Intestine 407 cells. Strain S102-9, serotype O5:H-, isolated from a calf with dysentery, contained six plasmids, one of which also hybridized with the CVD419 probe. Loss of pDEP12 coincided with reduced adhesion to HEp-2 or Intestine 407 cells cultured in vitro; reintroduction of this plasmid restored adhesiveness. Loss of the plasmid in strain S102-9 that hybridized with the CVD419 probe did not cause a decrease in adhesion. Accumulations of actin were seen in vitro in the fluorescence actin staining (FAS) test of strains S22-1, S102-9 and their derivatives, irrespective of the plasmid content of these strains or the prevalence of attached bacteria. Strain S22-1 and its plasmidless derivative caused AE lesions of equal severity in experimentally infected gnotobiotic piglets; piglets inoculated with an isolate from a healthy human or pig did not develop these lesions.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Agricultural and Food Research Council, Institute for Animal Health, Compton Laboratory, Newbury, Berkshire, UK.
authors.forename: G A
authors.initials: GA
authors.lastname: Hall
authors.forename: C R
authors.initials: CR
authors.lastname: Dorn
authors.forename: N
authors.initials: N
authors.lastname: Chanter
authors.forename: S M
authors.initials: SM
authors.lastname: Scotland
authors.forename: H R
authors.initials: HR
authors.lastname: Smith
authors.forename: B
authors.initials: B
authors.lastname: Rowe
mesh_terms: D018830:Adhesins, Escherichia coli; D000818:Animals; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D002417:Cattle; D002648:Child; D046508:Culture Techniques; D003603:Cytotoxins / Q000378:metabolism*; D015342:DNA Probes; D003967:Diarrhea / Q000209:etiology / Q000382:microbiology / Q000473:pathology; D004926:Escherichia coli / Q000378:metabolism* / Q000648:ultrastructure; D005856:Germ-Free Life; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D010957:Plasmids; D013552:Swine; D014709:Vero Cells
chemical_list: D018830:Adhesins, Escherichia coli; D001425:Bacterial Outer Membrane Proteins; D003603:Cytotoxins; D015342:DNA Probes