Effects of synapsin I and calcium/calmodulin-dependent protein kinase II on spontaneous neurotransmitter release in the squid giant synapse.
The molecular events that control synaptic vesicle availability in chemical synaptic junctions have not been fully clarified. Among the protein molecules specifically located in presynaptic terminals, synapsin I and calcium/calmodulin-dependent protein kinase II (CaM kinase II) have been shown to modulate evoked transmitter release in the squid giant synapse. In the present study, analysis of synaptic noise in this chemical junction was used to determine whether these proteins also play a role in the control of spontaneous and enhanced spontaneous transmitter release. Injections of dephosphorylated synapsin I into the presynaptic terminal reduced the rate of spontaneous and enhanced quantal release, whereas injection of phosphorylated synapsin I did not modify such release. By contrast CaM kinase II injection increased enhanced miniature release without affecting spontaneous miniature frequency. These results support the view that dephosphorylated synapsin I "cages" synaptic vesicles while CaM kinase II, by phosphorylating synapsin I, "decages" these organelles and increases their availability for release without affecting the release mechanism itself.
Lin
J W
JW
Marine Biological Laboratory, Woods Hole, MA 02543.
Sugimori
M
M
Llinás
R R
RR
McGuinness
T L
TL
Greengard
P
P
D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D049832:Decapodiformes; D005071:Evoked Potentials / Q000187:drug effects; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D008565:Membrane Proteins / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D011494:Protein Kinases / Q000494:pharmacology*; D013569:Synapses / Q000187:drug effects / Q000502:physiology*; D016704:Synapsins
D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D018377:Neurotransmitter Agents; D016704:Synapsins; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases