title: Cell and receptor requirements for streptococcal pyrogenic exotoxin T-cell mitogenicity.
abstract: Streptococcal pyrogenic exotoxins (SPEs) A, B, and C, like other members of the pyrogenic toxin family, are able to cause toxic shock-like syndromes. One of the major properties of these toxins is the ability to induce T-cell proliferation. Characterization of T cell mitogenicity associated with SPEs A, B, and C was undertaken. SPEs A, B, and C were mitogenic for C57BL10/SnJ and BALB/cWAT T cells, with activities differing in intensity depending on the mouse strain and toxin employed. SPE-induced, T-cell-proliferative activity was dependent on class II major histocompatibility complex molecules expressed on antigen-presenting cells. The abilities of SPEs A, B, and C to preferentially stimulate murine cells with certain T-cell receptor V beta s were investigated by fluorescence-activated cell sorter analysis. SPE A preferentially activated T cells expressing V beta 8 but not V beta 3, 6, or 11, while SPEs B and C preferentially stimulated T cells which did not express any of the tested V beta s.
authors.affiliation: Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455.
authors.forename: B A
authors.initials: BA
authors.lastname: Leonard
authors.forename: P K
authors.initials: PK
authors.lastname: Lee
authors.forename: M K
authors.initials: MK
authors.lastname: Jenkins
authors.forename: P M
authors.initials: PM
authors.lastname: Schlievert
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000938:Antigen-Presenting Cells / Q000187:drug effects / Q000276:immunology; D001426:Bacterial Proteins; D005098:Exotoxins / Q000378:metabolism / Q000633:toxicity*; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000187:drug effects; D008565:Membrane Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000378:metabolism / Q000633:toxicity*; D011948:Receptors, Antigen, T-Cell / Q000187:drug effects*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D005098:Exotoxins; D000949:Histocompatibility Antigens Class II; D008565:Membrane Proteins; D008934:Mitogens; D011948:Receptors, Antigen, T-Cell; C117980:SpeA protein, Streptococcus pyogenes; C027951:erythrogenic toxin