title: zeta-Crystallin is a major protein in the lens of Camelus dromedarius.
abstract: Camel (Camelus dromedarius) lenses contain a protein with an apparent subunit Mr 38,000 that constitutes approximately 8-13% of the total protein. The protein has been purified and has a native Mr 140,000 as determined by gel filtration. This is consistent with its being a tetramer. The protein reacts with antibodies raised against both guinea pig zeta-crystallin and peptides corresponding to amino acids 1-10 and 295-308, but not to antibodies raised against amino acids 320-328 of zeta-crystallin. Based on these criteria it is concluded that this protein, which is a major constituent of camel lens, is zeta-crystallin. This may be the first example of a protein (enzyme) being independently utilized as a crystallin in the lens of species from two mammalian orders.
authors.affiliation: Laboratory of Mechanisms of Ocular Diseases, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: D
authors.initials: D
authors.lastname: Garland
authors.forename: P V
authors.initials: PV
authors.lastname: Rao
authors.forename: A
authors.initials: A
authors.lastname: Del Corso
authors.forename: U
authors.initials: U
authors.lastname: Mura
authors.forename: J S
authors.initials: JS
authors.lastname: Zigler
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D002162:Camelus; D002850:Chromatography, Gel; D003459:Crystallins / Q000032:analysis* / Q000276:immunology; D007908:Lens, Crystalline / Q000737:chemistry*; D008970:Molecular Weight
chemical_list: D000906:Antibodies; D003459:Crystallins