Analysis of the int-1, int-2, c-myc, and neu oncogenes in human breast carcinomas.
We have examined the DNA obtained from 100 primary breast carcinomas for oncogene markers which might have predictive value for poor prognosis. Ninety-six of the tumors were analyzed for the presence of restriction fragment length polymorphisms (RFLPs) previously identified in the int-2 gene. An 8.4-kilobase BamHI fragment and a 3.9-kilobase PstI fragment specific for the int-2 gene, in the absence of other restriction fragments, was found in 17 of 50 (34%) lymph node-negative patients and in 27 of 44 (61%) lymph node-positive patients. This combination of int-2 RFLPs (8.4/3.9) was found in a significantly different proportion (P = 0.02) of patients with greater than 3 positive lymph nodes compared to patients with fewer positive lymph nodes, suggesting that these RFLPs may be valuable for distinguishing among node-negative patients for chemotherapy. In contrast, the observed low frequency of int-1, int-2, neu, and c-myc amplification limited their usefulness as clinical predictors of disease recurrence.
Meyers
S L
SL
Department of Microbiology, University of Texas, Austin 78712.
O'Brien
M T
MT
Smith
T
T
Dudley
J P
JP
D001943:Breast Neoplasms / Q000235:genetics*; D005260:Female; D051518:Fibroblast Growth Factor 3; D005346:Fibroblast Growth Factors; D005784:Gene Amplification; D005838:Genotype; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes; D018719:Erb-b2 Receptor Tyrosine Kinases; D051153:Wnt Proteins; D051155:Wnt1 Protein; D029961:Zebrafish Proteins
D051518:Fibroblast Growth Factor 3; D005346:Fibroblast Growth Factors; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D018719:Erb-b2 Receptor Tyrosine Kinases; D051153:Wnt Proteins; D051155:Wnt1 Protein; D029961:Zebrafish Proteins; C496444:FGF3 protein, human; C489200:fgf3 protein, zebrafish