title: Alpha 2-adrenoceptor-mediated autoinhibition of sympathetic transmitter release in guinea-pig vas deferens studied by intracellular and focal extracellular recording of junction potentials and currents.
abstract: Excitatory junction potentials (e.j.ps; intracellular electrodes) and excitatory junction currents (e.j.cs; extracellular electrodes) elicited by stimulation (20 pulses at 1 Hz every minute) of the hypogastric nerve trunk were recorded from guinea-pig isolated vas deferens. Intracellular recording. At a variety of stimulation intensities, bath-applied yohimbine (0.1-1 mumol/l) did not change the first one to three e.j.ps in a train but increased the amplitude of subsequent e.j.ps. The effect of yohimbine was abolished in tissues from reserpine-pretreated guinea pigs. Bath-applied desipramine (0.1 mumol/l) diminished the amplitude of all but the first one to three e.j.ps in a train.--Extracellular recording. Yohimbine (0.1-1 mumol/l), when applied locally through the recording suction electrode, increased the number of e.j.cs per given number of stimuli, i.e., enhanced the probability of occurrence of e.j.cs. When desipramine (0.1 mumol/l) was present both in the bath and in the recording electrode, the probability of the occurrence of e.j.cs was decreased. In the presence of desipramine, yohimbine (0.1-1 mumol/l) increased the number of e.j.cs even more markedly. Neither the nerve terminal impulse nor the number of spontaneous e.j.cs was changed by yohimbine. A mixture of tetraethylammonium (2 mmol/l) and 4-aminopyridine (0.2 mmol/l), when applied locally, both increased the number of e.j.cs and changed markedly the shape of the nerve terminal impulse.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: University Department of Pharmacology, Oxford, UK.
authors.forename: J A
authors.initials: JA
authors.lastname: Brock
authors.forename: T C
authors.initials: TC
authors.lastname: Cunnane
authors.forename: K
authors.initials: K
authors.lastname: Starke
authors.forename: C F
authors.initials: CF
authors.lastname: Wardell
mesh_terms: D000818:Animals; D004558:Electric Stimulation; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism*; D009469:Neuromuscular Junction / Q000187:drug effects / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D015221:Potassium Channels / Q000187:drug effects; D011942:Receptors, Adrenergic, alpha / Q000502:physiology*; D013564:Sympathetic Nervous System / Q000378:metabolism* / Q000502:physiology; D014649:Vas Deferens / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D015016:Yohimbine / Q000494:pharmacology
chemical_list: D018377:Neurotransmitter Agents; D015221:Potassium Channels; D011942:Receptors, Adrenergic, alpha; D015016:Yohimbine