Saturation kinetics of palmitate uptake in perfused skeletal muscle.
We investigated the kinetics of palmitate uptake in a physiological skeletal muscle preparation by using the isolated perfused rat hindquarter. When plotted against the unbound plasma palmitate concentration, palmitate uptake displayed a simple Michaelis-Menten relation with a calculated Vmax and Km of 16.3 nmol.min-1.g-1 and 0.06 mumol.l-1, respectively. These results show that, as in isolated cell systems, uptake of free fatty acids in perfused skeletal muscle follows saturation kinetics consistent with carrier-mediated membrane transport of free fatty acids.
Turcotte
L P
LP
August Krogh Institute, University of Copenhagen, Denmark.
Kiens
B
B
Richter
E A
EA
D000818:Animals; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D009132:Muscles / Q000378:metabolism*; D010168:Palmitates / Q000378:metabolism* / Q000493:pharmacokinetics; D051381:Rats; D011919:Rats, Inbred Strains; D012044:Regression Analysis
D005230:Fatty Acids, Nonesterified; D010168:Palmitates