title: Dimerization of the P-glycoprotein in membranes.
abstract: Plasma membranes from a CHO cell line, CHRC5, which exhibits multidrug resistance was studied using radiation inactivation analysis. The P-glycoprotein content of the membrane was determined by Western blots. Irradiation resulted in the loss of P-glycoprotein. The dependence of this loss on radiation dose corresponded to a target size of 250 kDa which is the molecular mass of a dimer of the P-glycoprotein. This is strong evidence to indicate that the P-glycoprotein self associates in the membrane.
authors.affiliation: Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada.
authors.forename: D
authors.initials: D
authors.lastname: Boscoboinik
authors.forename: M T
authors.initials: MT
authors.lastname: Debanne
authors.forename: A R
authors.initials: AR
authors.lastname: Stafford
authors.forename: C Y
authors.initials: CY
authors.lastname: Jung
authors.forename: R S
authors.initials: RS
authors.lastname: Gupta
authors.forename: R M
authors.initials: RM
authors.lastname: Epand
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism / Q000528:radiation effects*; D006224:Cricetinae; D004351:Drug Resistance; D004591:Electrophoresis, Polyacrylamide Gel; D008562:Membrane Glycoproteins / Q000378:metabolism* / Q000528:radiation effects; D013329:Structure-Activity Relationship
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D008562:Membrane Glycoproteins