Evaluation of the Staph-Zym system with staphylococci isolated from bovine intramammary infections.
A total of 148 staphylococci isolated from bovine intramammary infections were used to evaluate the Staph-Zym system (ROSCO, Taastrup, Denmark). The overall accuracy of the system was 91.9%. The system correctly identified all strains of Staphylococcus aureus, Staphylococcus simulans, and Staphylococcus xylosus and 95% of Staphylococcus intermedius strains. Of 33 Staphylococcus hyicus strains, 31 (93.9%) were classified correctly by the Staph-Zym system, as well as 8 (80%) of 10 Staphylococcus chromogenes strains. All 11 Staphylococcus epidermidis strains and the 1 Staphylococcus haemolyticus strain included in the study were identified, but the Staph-Zym system had difficulty distinguishing strains of Staphylococcus warneri and Staphylococcus hominis from other species in the S. epidermidis group. The Staph-Zym system correctly identified all six S. xylosus strains and two of three Staphyloccus sciuri strains. The Staph-Zym system was considered an acceptable alternative to conventional methods for identification of bovine mammary gland isolates.
Watts
J L
JL
Mastitis Research Laboratory, Louisiana State University Agricultural Center Homer 71040.
Washburn
P J
PJ
D000818:Animals; D001431:Bacteriological Techniques / Q000295:instrumentation*; D002417:Cattle; D004798:Enzymes; D005069:Evaluation Studies as Topic; D008414:Mastitis, Bovine / Q000175:diagnosis / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000662:veterinary*; D013210:Staphylococcus / Q000302:isolation & purification*
D004798:Enzymes