[Secondary messengers in the hormonal regulation of the functional activity of the main and mucoid cells in the stomach].
The messenger role of Ca+2, cyclic nucleotides and inositol triphosphates in the stimulation of pepsinogen and mucous secretion were studied using isolated pig [correction of nug] gastric chief cells and guinea pig mucous cells, resp. Pepsinogen secretion was stimulated by agents either working at the postreceptor adenylate cyclase (AC) level (db-cAMP, forskolin) or after 12-o-tetradecanoyl-phorbol-13-acetate (TPA) stimulation of protein kinase C (PK C). Similar secretory effects were observed with histamine (H), carbachol (C) and cholecystokinin (CCK). [Ca-2] in was elevated by C and by CCK, but not by H in both types of cells. Like TPA, both C and CCK, but not H, stimulated the Ca+2-sensitive particulate PK C. H increased the activity of cAMP-dependent PK A. PGE2, C and CCK were found to increase inositol-1,4,5-triphosphate content in mucous cells. The findings indicate that two pathways of the regulation of pepsinogen and mucous secretion (AC-cAMP-PK C and phosphoinositol breakdown cascade) can act synergistically.
Bersimbaev
R I
RI
Tairov
M M
MM
Beinborn
M
M
Beil
W
W
Sewing
K F
KF
D000262:Adenylyl Cyclases / Q000187:drug effects / Q000502:physiology; D000818:Animals; D002478:Cells, Cultured / Q000187:drug effects / Q000502:physiology; D000242:Cyclic AMP / Q000502:physiology; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism; D005768:Gastrointestinal Hormones / Q000502:physiology*; D006168:Guinea Pigs; D018377:Neurotransmitter Agents / Q000502:physiology; D011493:Protein Kinase C / Q000187:drug effects / Q000502:physiology; D011494:Protein Kinases / Q000187:drug effects / Q000502:physiology; D015290:Second Messenger Systems / Q000187:drug effects / Q000502:physiology*; D013270:Stomach / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D013552:Swine
D005768:Gastrointestinal Hormones; D018377:Neurotransmitter Agents; D000242:Cyclic AMP; D011494:Protein Kinases; D011493:Protein Kinase C; D000262:Adenylyl Cyclases