In vivo anti-complementary activities of the cobra venom factors from Naja naja and Naja haje.
The kinetics of complement (C) depletion and recovery of C levels upon injection of BALB/c mice with cobra venom factors (CVF), from N. naja (C3- and C5-depleting) and N. haje (selectively C3-depleting) were studied. The animals received i.p. or i.v. injections of either of the two preparations. CH50 and hemolytic C3 and C5 levels were followed as parameters of residual complement activity. N. naja CVF turned out to be as efficient in depleting total complement activity as N. haje CVF. Decreased CH50 values could largely be ascribed to C3 depletion. Complement consumption after N. naja CVF, however, lasted longer than after N. haje CVF administration. Estimated functional half-lives of N. naja and N. haje CVF were 11.5 and 4.5 h, respectively. Inhibition ELISAs showed that, after in vivo administration of either of the two CVF preparations, antigenic C3 and C5 kept circulating for days.
Van den Berg
C W
CW
Eijkman-Winkler Laboratory of Medical Microbiology, Department of Experimental Microbiology, Faculty of Medicine, University of Utrecht, The Netherlands.
Aerts
P C
PC
Van Dijk
H
H
D000818:Animals; D003176:Complement C3 / Q000378:metabolism; D003182:Complement C5 / Q000378:metabolism; D003165:Complement System Proteins / Q000378:metabolism*; D004546:Elapid Venoms / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
D003176:Complement C3; D003182:Complement C5; D004546:Elapid Venoms; D003165:Complement System Proteins