An FM3A mutant, G258, with a mutation that affects both cell growth and oligosaccharide-lipid synthesis.
I have previously isolated a temperature-sensitive FM3A mutant (G258) defective in asparagine-linked glycosylation. G258 shows not only the temperature sensitivity for cell growth, but also temperature sensitivity for synthesis of oligosaccharide-lipid (Nishikawa, Y. (1984) J. Cell. Physiol. 119, 260-266). In the present study, I isolated revertants for cell growth from G258 cells. All three growth revertants also showed reversion on the synthesis of oligosaccharide-lipid. These results imply that the temperature sensitivity for oligosaccharide-lipid synthesis of G258 couples with the temperature sensitivity for cell growth of the mutant. A possible mechanism of the coupling between impaired oligosaccharide-lipid synthesis and growth arrest of G258 cells at 39 degrees C is discussed.
Nishikawa
Y
Y
Tokyo Metropolitan Institute of Medical Science, Japan.
D000818:Animals; D001216:Asparagine / Q000378:metabolism; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D006023:Glycoproteins / Q000096:biosynthesis; D006031:Glycosylation; D008325:Mammary Neoplasms, Experimental / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009154:Mutation; D009844:Oligosaccharides / Q000096:biosynthesis*; D013696:Temperature
D006023:Glycoproteins; D009844:Oligosaccharides; D001216:Asparagine