The effects of calcium and calcium channel blockade on peptide YY and neuropeptide Y release.
The secretory response of many gastrointestinal hormones has been demonstrated to be mediated by way of calcium-dependent pathways. The present study was done to assess the effects of infusions of calcium (15 milligrams per kilogram per hour) and verapamil (5 milligrams per kilogram per hour) on circulatory and ileal intraluminal release of peptide YY and neuropeptide Y. Five dogs weighing 25 kilograms had distal ileal Thiry-Vella segments of 25 centimeters surgically created. After a two week recovery period, each dog was tested after overnight fasting. The dogs were given one hour infusions of either calcium or verapamil after basal measurements. One hour of postinfusion measurements completed the study. Intravenous calcium did not affect circulating peptide YY or neuropeptide Y, but did significantly increase recoverable ileal peptide YY. Verapamil administration significantly increased both circulating peptide YY and neuropeptide Y levels. The results of our data suggest an independent release of intraluminal and intravascular peptide YY as well as modulation of the circulatory release of both peptide YY and neuropeptide Y by cell membrane calcium channel blockade.
McFadden
D W
DW
Department of Surgery, University of Cincinnati College of Medicine, Ohio 45267-0558.
Rudnicki
M
M
Fischer
J E
JE
D000818:Animals; D002118:Calcium / Q000008:administration & dosage / Q000493:pharmacokinetics*; D004285:Dogs; D004334:Drug Administration Schedule; D004353:Drug Evaluation, Preclinical; D007082:Ileum / Q000187:drug effects* / Q000378:metabolism; D007262:Infusions, Intravenous; D009479:Neuropeptides / Q000097:blood / Q000378:metabolism*; D019894:Peptide YY; D010455:Peptides / Q000097:blood / Q000378:metabolism*; D014700:Verapamil / Q000008:administration & dosage / Q000493:pharmacokinetics*
D009479:Neuropeptides; D010455:Peptides; D019894:Peptide YY; D014700:Verapamil; D002118:Calcium