Interleukin 6, a possible autocrine growth and differentiation factor for the human megakaryocytic cell line, CMK.
CMK is a human cell line derived from a megakaryoblastic leukaemia. It has characteristics of the megakaryocytic lineage, such as the presence of platelet peroxidase, membrane glycoproteins (GP)Ib and GPIIb/IIIa, alpha-granules, and demarcation membranes. The cell line proliferates autonomously in serum-containing medium. Here we report that the cell line expresses the gene for IL-6 and releases small quantities of the cytokine into the medium. Addition of exogenous IL-6 to cultures seeded into medium was found to promote growth of the cells. Conversely, addition of a neutralizing anti-IL-6 antibody inhibited cell growth. These data support the notion that autocrine IL-6 is one of the factors accounting for autonomous growth of the cell line.
Fuse
A
A
Department of Microbiology, School of Medicine, Chiba University, Japan.
Kakuda
H
H
Shima
Y
Y
Van Damme
J
J
Billiau
A
A
Sato
T
T
D015152:Blotting, Northern; D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis / Q000502:physiology*; D007947:Leukemia, Megakaryoblastic, Acute / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured
D015850:Interleukin-6