title: Hemolytic activity of adenylate cyclase toxin from Bordetella pertussis.
abstract: Adenylate cyclase (AC) toxin from B. pertussis enters eukaryotic cells where it produces supraphysiologic levels of cAMP. Purification of AC toxin activity [(1989) J. Biol. Chem. 264, 19279] results in increasing potency of hemolytic activity and electroelution of the 216-kDa holotoxin yields a single protein with AC enzymatic, toxin and hemolytic activities. AC toxin and E. coli hemolysin, which have DNA sequence homology [(1988) EMBO J. 7, 3997] are immunologically cross-reactive. The time courses of hemolysis elicited by the two molecules are strikingly different, however, with AC toxin eliciting cAMP accumulation with rapid onset, but hemolysis with a lag of greater than or equal to 45 min. Finally, osmotic protection experiments indicate that the size of the putative pore produced by AC toxin is 3-5-fold smaller than that of E. coli hemolysin.
authors.affiliation: Department of Medicine, University of Virginia School of Medicine, Charlottesville 22908.
authors.forename: I E
authors.initials: IE
authors.lastname: Ehrmann
authors.forename: M C
authors.initials: MC
authors.lastname: Gray
authors.forename: V M
authors.initials: VM
authors.lastname: Gordon
authors.forename: L S
authors.initials: LS
authors.lastname: Gray
authors.forename: E L
authors.initials: EL
authors.lastname: Hewlett
mesh_terms: D037361:Adenylate Cyclase Toxin; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D015153:Blotting, Western; D001886:Bordetella pertussis / Q000201:enzymology*; D002118:Calcium / Q000378:metabolism; D003429:Cross Reactions; D004912:Erythrocytes / Q000187:drug effects; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; D006461:Hemolysis / Q000187:drug effects*; D012756:Sheep; D010566:Virulence Factors, Bordetella / Q000235:genetics / Q000633:toxicity*
chemical_list: D037361:Adenylate Cyclase Toxin; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D010566:Virulence Factors, Bordetella; D002118:Calcium