Demonstration of a cell wall antigen cross-reacting with cryptococcal polysaccharide in experimental disseminated trichosporonosis.
Patients with disseminated infections caused by Trichosporon beigelii have a circulating antigen that cross-reacts with the polysaccharide capsule of Cryptococcus neoformans. We studied the localization of this antigen by immunoelectron microscopy in a rabbit model of experimental disseminated trichosporonosis. Deparaffinized lung sections were examined by using a murine monoclonal anti-cryptococcal polysaccharide antibody and colloidal gold particles coated with goat antibody to murine immunoglobulin G. Antigen that cross-reacted with the monoclonal antibody was observed in the T. beigelii cell wall and in a fibrillar matrix extending from the cell wall.
Melcher
G P
GP
Department of Medicine, Wilford Hall U.S. Air Force Medical Center, Lackland Air Force Base, Texas 78236.
Reed
K D
KD
Rinaldi
M G
MG
Lee
J W
JW
Pizzo
P A
PA
Walsh
T J
TJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000946:Antigens, Fungal / Q000032:analysis*; D002473:Cell Wall / Q000276:immunology* / Q000648:ultrastructure; D003429:Cross Reactions; D003455:Cryptococcus neoformans / Q000276:immunology*; D004195:Disease Models, Animal; D007074:Immunoglobulin G / Q000276:immunology; D016253:Microscopy, Immunoelectron; D009181:Mycoses / Q000175:diagnosis* / Q000473:pathology; D011134:Polysaccharides / Q000276:immunology*; D011817:Rabbits; D014250:Trichosporon / Q000276:immunology* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal; D007074:Immunoglobulin G; D011134:Polysaccharides; C014426:cryptococcal polysaccharide