title: Tetrahymena 14-nm filament-forming protein has citrate synthase activity.
abstract: The Tetrahymena 14-nm filament-forming protein (49K protein) is a structural protein involved in oral morphogenesis and in pronuclear behavior during conjugation. Cloning the 49K protein gene from a Tetrahymena thermophila cDNA library, we found that its primary structure exhibits a high sequence identity (51.5%) with porcine heart citrate synthase and retains functional domains. The 49K protein actually possesses citrate synthase activity, and is detected in mitochondria. These results suggest that the 49K protein has dual functions as both a respiratory enzyme and a structural protein in the cytoskeleton.
authors.affiliation: Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Numata
authors.forename: T
authors.initials: T
authors.lastname: Takemasa
authors.forename: I
authors.initials: I
authors.lastname: Takagi
authors.forename: M
authors.initials: M
authors.lastname: Hirono
authors.forename: H
authors.initials: H
authors.lastname: Hirano
authors.forename: J
authors.initials: J
authors.lastname: Chiba
authors.forename: Y
authors.initials: Y
authors.lastname: Watanabe
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002950:Citrate (si)-Synthase / Q000378:metabolism*; D003001:Cloning, Molecular; D005455:Fluorescent Antibody Technique; D008969:Molecular Sequence Data; D009206:Myocardium / Q000201:enzymology; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D013768:Tetrahymena / Q000201:enzymology / Q000235:genetics / Q000502:physiology*
chemical_list: D015800:Protozoan Proteins; C067125:49K protein, Tetrahymena; D002950:Citrate (si)-Synthase