Factors influencing the adherence of strains of Streptococcus bovis and Escherichia coli isolated from ruminal epithelium.
Two strains of Streptococcus bovis (A1 and A5) and one strain of Escherichia coli (0141:H28) isolated from the surface of bovine ruminal mucous epithelium were examined for adherence to isolated and cultured ruminal epithelial cells. The E. coli adhered to the target cell by means of fimbriae, which had several common properties with type 1 common fimbriae and caused mannose-sensitive haemagglutination. The A1 strain of S. bovis was devoid of fimbriae and its adherence to the epithelial surface was not inhibited by treatment with sugars or phenol-treated bacterial membrane from the same organism. It was therefore postulated that the bacterial glycocalyx of the S. bovis organisms acted as ligand. The extent of bacterial adherence depended on the state of differentiation of the target cell in both the isolated and the cultured ruminal cell systems. The receptors for both adherent bacterial species were in all probability associated with the glycocalyx of the target cells.
Semjén
G
G
Department of Pharmacology, University of Veterinary Sciences, Budapest, Hungary.
Gálfi
P
P
D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D002417:Cattle; D002478:Cells, Cultured; D004848:Epithelium / Q000382:microbiology; D004926:Escherichia coli / Q000378:metabolism* / Q000648:ultrastructure; D010861:Fimbriae, Bacterial / Q000378:metabolism; D005753:Gastric Mucosa / Q000382:microbiology; D006023:Glycoproteins / Q000378:metabolism; D006384:Hemagglutination; D008297:Male; D008854:Microscopy, Electron; D011134:Polysaccharides / Q000378:metabolism; D012417:Rumen / Q000382:microbiology*; D013291:Streptococcus / Q000378:metabolism* / Q000648:ultrastructure
D006023:Glycoproteins; D011134:Polysaccharides