Molecular mechanisms underlying the expression of the human HOX-5.1 gene.
The complex mechanisms underlying homeobox genes expression involve regulation at transcriptional, post-transcriptional and translational levels. The multiple transcripts of the human HOX-5.1 gene are expressed differentially in tissue- and stage-specific patterns during embryogenesis, and differentially induced by retinoic acid (RA) in human embryonal carcinoma (EC) NT2/D1 cells. We have sequenced 6.3 Kb of the genomic region containing the HOX-5.1 gene and analyzed its mechanisms of expression. Two alternative promoters underlie the transcription of two classes of HOX-5.1-specific mRNAs. These classes differ in tissue and subcellular distribution, induction by RA, structure of the 5'-UT region and mRNA stability: these features are compatible with a differential function of the two classes of transcripts in embryogenesis.
Cianetti
L
L
Department of Hematology and Oncology, Istituto Superiore di Sanità, Rome, Italy.
Di Cristofaro
A
A
Zappavigna
V
V
Bottero
L
L
Boccoli
G
G
Testa
U
U
Russo
G
G
Boncinelli
E
E
Peschle
C
C
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D004622:Embryo, Mammalian / Q000378:metabolism; D054278:Embryonal Carcinoma Stem Cells; D005786:Gene Expression Regulation; D005801:Genes, Homeobox; D006801:Humans; D008969:Molecular Sequence Data; D014411:Neoplastic Stem Cells; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D015183:Restriction Mapping; D012260:Ribonucleases / Q000378:metabolism; D014158:Transcription, Genetic; D014212:Tretinoin / Q000494:pharmacology
D014212:Tretinoin; D012260:Ribonucleases