A novel Saccharomyces cerevisiae secretory mutant possesses a thermolabile phosphomannose isomerase.
A temperature-sensitive mutant of Saccharomyces cerevisiae was identified which at the restrictive temperature of 37 degrees C is unable to secrete a number of cell wall-associated proteins and thus resembles previously reported sec mutants. In contrast to other sec mutants, however, both the temperature-sensitive growth and the secretion defects can be repaired by the addition of D-mannose to growth media. We show that the mutant possesses a single, apparently recessive mutation which leads to the production of a thermolabile phosphomannose isomerase.
Payton
M A
MA
Glaxo Institute for Molecular Biology, Geneva, Switzerland.
Rheinnecker
M
M
Klig
L S
LS
DeTiani
M
M
Bowden
E
E
D006358:Hot Temperature; D008358:Mannose / Q000378:metabolism; D008359:Mannose-6-Phosphate Isomerase / Q000235:genetics*; D009154:Mutation; D011489:Protein Denaturation; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology* / Q000235:genetics
D008359:Mannose-6-Phosphate Isomerase; D008358:Mannose