Immunolocation of TNF-alpha/cachectin in human melanoma cells: studies on co-cultivated malignant melanoma.
We have investigated the ability of metastatic cells to produce the macrophage cytokine, TNF-alpha/cachectin, as these cells have macrophage-like properties such as infiltration and migration. We looked for TNF-alpha/cachectin in three tumor cell lines derived from human malignant melanomas and six co-cultivated malignant melanomas derived, in vitro, from these three cell lines plus angioma fibroblasts. Immunohistochemistry with an anti-TNF-alpha/cachectin monoclonal antibody showed that TNF-alpha/cachectin was produced by two of the three parent melanoma cell lines. All the tumor cells in both the co-cultivated malignant melanomas and their in vitro tumorous nodules produced TNF-alpha/cachectin, even those derived from the melanoma cell line, which originally did not. The results clearly show that TNF-alpha/cachectin can be produced by non-hematopoietic tumor cells. A co-cultivated tumor model prepared from other types of human tumor cell lines promises to provide a useful tool for exploring the relationship between TNF-alpha/cachectin and oncogenesis.
Lugassy
C
C
Laboratoire d'Oncologie, Hôpital Tarnier-Cochin, Paris, France.
Escande
J P
JP
D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal; D015152:Blotting, Northern; D002450:Cell Communication; D002460:Cell Line; D015342:DNA Probes; D005260:Female; D006391:Hemangioma / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008545:Melanoma / Q000473:pathology*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D014407:Tumor Cells, Cultured / Q000166:cytology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis* / Q000235:genetics
D000911:Antibodies, Monoclonal; D015342:DNA Probes; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha