Type of fimbriation determines adherence of Klebsiella bacteria to human epithelial cells.
Clinical isolates of three Klebsiella strains (encapsulated and nonencapsulated mutants) with type 1 (mannose-sensitive, MS+MR-), type 3 (mannose-resistant, MS-MR+) and type 1 and 3 (MS+MR+) fimbriae were investigated for their ability to adhere to epithelial cells. Considerable adhesion to human buccal, tracheal, pulmonary and uroepithelial cells could be demonstrated. Independent of encapsulation and type of epithelial cells, adhesion of MS+MR+ (type 1.3) fimbriated Klebsiella bacteria was significantly stronger than adhesion of microorganisms carrying only type 1 (MS+MR-) or type 3 (MS-MR+) fimbriate, respectively. Adherence of nonencapsulated type 1 and 3 (MS+MR+) fimbriated Klebsiella bacteria to mammalian cells was significantly inhibited in the presence of D-mannose. Certain carbohydrates (D-glucose, D-galactose) did not interfere with this adhesion process.
Würker
M
M
Institute of Medical Microbiology and Hygiene, University of Cologne.
Beuth
J
J
Ko
H L
HL
Przondo-Mordarska
A
A
Pulverer
G
G
D001422:Bacterial Adhesion; D002460:Cell Line; D002478:Cells, Cultured; D004847:Epithelial Cells; D004848:Epithelium / Q000382:microbiology*; D010861:Fimbriae, Bacterial / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007709:Klebsiella / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D008168:Lung / Q000166:cytology / Q000382:microbiology; D008358:Mannose / Q000494:pharmacology; D009061:Mouth Mucosa / Q000166:cytology / Q000382:microbiology; D014132:Trachea / Q000166:cytology / Q000382:microbiology; D014551:Urinary Tract / Q000166:cytology / Q000382:microbiology
D008358:Mannose