title: Human rib bone marrow mononuclear cells spontaneously synthesize and secrete IgE in vitro.
abstract: We have examined spontaneous secretion of IgE by human rib bone marrow mononuclear cells (MNC). Bone marrow MNC from nine out of 12 rib specimens synthesized and secreted substantial amounts of IgE during 14 days of in vitro culture. The 14-day supernatants from these bone marrow MNC contained a mean of 2589 pg/ml of IgE (n = 12) with a maximum production of 15,408 pg/ml of IgE compared with small amounts of IgE (80-200 pg/ml) produced by similarly cultured normal and inflammatory bowel disease intestinal lamina propria MNC. Using two rib specimens, time-course studies revealed spontaneous secretion of IgE to be minimal during the first 2 days of culture (152 pg/ml), followed by a steady increase between days 4 (517 pg/ml) and 14 (3588 pg/ml). The addition of pokeweed mitogen resulted in 72% suppression of spontaneous IgE production by bone marrow MNC. The bone marrow MNC isolated from the ribs consisted of 22% Leu12+ (B) cells of which 3.2% were surface IgE positive. Staining for cytoplasmic immunoglobulin revealed 1% of the bone marrow MNC to be cytoplasmic IgE+. The presence of IgE-bearing and IgE-secreting MNC in human bone marrow is consistent with the observation that allergen-specific IgE-mediated hypersensitivity is adoptively transferred by human bone marrow transplantation and demonstrates the usefulness of human bone marrow MNC for examination of IgE secretory and regulatory events.
authors.affiliation: Gastroenterology Section, University of Pennsylvania School of Medicine, Philadelphia 19104-6144.
authors.forename: R P
authors.initials: RP
authors.lastname: MacDermott
authors.forename: G A
authors.initials: GA
authors.lastname: Jendrisak
authors.forename: G S
authors.initials: GS
authors.lastname: Nash
authors.forename: S
authors.initials: S
authors.lastname: Schreiber
authors.forename: M J
authors.initials: MJ
authors.lastname: Bertovich
authors.forename: M
authors.initials: M
authors.lastname: Nahm
authors.forename: M
authors.initials: M
authors.lastname: Nonaka
authors.forename: P
authors.initials: P
authors.lastname: Fitzgerald
authors.forename: D H
authors.initials: DH
authors.lastname: Katz
authors.forename: J F
authors.initials: JF
authors.lastname: Marcelletti
mesh_terms: D000328:Adult; D000368:Aged; D001853:Bone Marrow / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis / Q000378:metabolism*; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011043:Pokeweed Mitogens; D011863:Radioimmunoassay; D012272:Ribs / Q000166:cytology / Q000276:immunology*
chemical_list: D007132:Immunoglobulin Isotypes; D011043:Pokeweed Mitogens; D007073:Immunoglobulin E