title: Role of Ca2+ and prostaglandin in regulation of active Na+ transport in frog skin.
abstract: 1. The role of prostaglandins and intracellular Ca2+ in regulation of active transepithelial sodium transport in frog skin were studied by examinations of effects of the calcium ionophore A23187 on short-circuit current (SCC) and intracellular voltage. 2. A23187 and arachidonic acid induced a marked increase in both SCC and prostaglandin E2 synthesis. 3. In indomethacin treated skins A23187 did not stimulate but on the contrary inhibited the basal SCC. 4. The A23187-induced increase in SCC was associated with a decrease in the fractional resistance of the apical membrane and a depolarization of the cells. 5. In skins pretreated with indomethacin, the A23187 induced inhibition of SCC coincided with a slight hyperpolarization of the cellular potential and an increase in fractional resistance of the apical membrane.
authors.affiliation: Institute of Life Sciences and Chemistry, University of Roskilde, Denmark.
authors.forename: H F
authors.initials: HF
authors.lastname: Bjerregaard
authors.forename: R
authors.initials: R
authors.lastname: Nielsen
mesh_terms: D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D015232:Dinoprostone / Q000096:biosynthesis; D005260:Female; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D011453:Prostaglandins / Q000378:metabolism*; D011894:Rana pipiens; D012867:Skin / Q000187:drug effects / Q000378:metabolism; D012964:Sodium / Q000378:metabolism*
chemical_list: D011453:Prostaglandins; D000001:Calcimycin; D012964:Sodium; D015232:Dinoprostone; D002118:Calcium; D007213:Indomethacin