title: Mechanical properties of adult feline ventricular myocytes in culture.
abstract: The contractile and electrophysiological properties of cultured adult feline ventricular myocytes were studied. Cells were field stimulated and contraction was measured using a video-based edge detector. The magnitude of contraction decreased by 36% and the rate of contraction decreased by 52% 2 h after the cells were plated on laminin-coated cover slips. The magnitude and rate of contraction then remained stable for 1 wk. The duration of contraction prolonged and a second component to the twitch frequently, but not invariably, developed after 5 days in culture. This was associated with prolongation of the action potential duration. After 7 days in culture, cells could be divided into two groups based on resting membrane potential. Norepinephrine increased the magnitude of contraction for 5 days after plating. Cultured ventricular myocytes became unresponsive to the effects of norepinephrine after 7 days. Adult cardiac myocytes maintained in primary culture continue to respond to field stimulation and retain many contractile properties for up to 7 days; however, the functional characteristics of these cells do not remain uniform during this time period.
authors.affiliation: Department of Medicine, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
authors.forename: P S
authors.initials: PS
authors.lastname: Pollack
authors.forename: N L
authors.initials: NL
authors.lastname: Carson
authors.forename: H B
authors.initials: HB
authors.lastname: Nuss
authors.forename: T A
authors.initials: TA
authors.lastname: Marino
authors.forename: S R
authors.initials: SR
authors.lastname: Houser
mesh_terms: D000818:Animals; D002415:Cats; D002469:Cell Separation; D002478:Cells, Cultured; D004553:Electric Conductivity / Q000187:drug effects / Q000502:physiology; D006352:Heart Ventricles / Q000166:cytology / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009200:Myocardial Contraction / Q000187:drug effects / Q000502:physiology; D009206:Myocardium / Q000166:cytology*; D009638:Norepinephrine / Q000494:pharmacology; D013997:Time Factors; D016276:Ventricular Function
chemical_list: D009638:Norepinephrine