title: Stereoselective effect of warfarin and bilirubin on the binding of 5-(o-chlorophenyl)-1,3-dihydro-3-methyl-7-nitro-2H-1,4-benzodiazepin-2- one enantiomers to human serum albumin.
abstract: The binding of the title benzodiazepine enantiomers and its modulation by warfarin and bilirubin were studied by chromatography on human serum albumin (HSA) immobilized on Sepharose 4B, and also by a combination of ultrafiltration and circular dichroism (UF-CD) methods. In the absence of warfarin and bilirubin the binding of the benzodiazepine was not stereoselective. (S)-Benzodiazepine and (S)-warfarin mutually increased the binding of each other, while the binding of (R)-benzodiazepine was preferentially enhanced on HSA saturated with bilirubin.
authors.affiliation: Central Research Institute for Chemistry, Hungarian Academy of Sciences, Budapest.
authors.forename: I
authors.initials: I
authors.lastname: Fitos
authors.forename: J
authors.initials: J
authors.lastname: Visy
authors.forename: A
authors.initials: A
authors.lastname: Magyar
authors.forename: J
authors.initials: J
authors.lastname: Kajtár
authors.forename: M
authors.initials: M
authors.lastname: Simonyi
mesh_terms: D014151:Anti-Anxiety Agents; D001569:Benzodiazepines; D001663:Bilirubin / Q000494:pharmacology*; D002846:Chromatography, Affinity; D002942:Circular Dichroism; D002998:Clonazepam / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D010600:Pharmacology; D012709:Serum Albumin / Q000378:metabolism*; D013237:Stereoisomerism; D014859:Warfarin / Q000494:pharmacology*
chemical_list: D014151:Anti-Anxiety Agents; D012709:Serum Albumin; D001569:Benzodiazepines; C014268:ID 690; D002998:Clonazepam; D014859:Warfarin; D001663:Bilirubin