Beta-endorphin-like and adrenocorticotropin-like materials in turtle heart and intestine.
1. Turtle heart and intestine acetone powders were extracted with an acetone-water-HCl mixture. An acid acetone powder resulted by adding a copious volume of acetone to the extract. The powder was subjected to gel filtration on Sephadex G-25 and ion exchange chromatography on CM-cellulose. 2. In turtle heart, corticotropin-like bioactivity was distributed among chromatographic fractions (derived from material unretarded on Sephadex G-25) unadsorbed and adsorbed on CM-cellulose. The highest opiate receptor binding activity and beta-endorphin-like immunoreactivity were adsorbed on CM-cellulose. 3. In turtle intestine, corticotropin-like bioactivity was absent. Opiate receptor binding activity was present in fractions unretarded as well as in fractions retarded on Sephadex G-25, indicating a molecular weight of greater and smaller than 5000 respectively. 4. The highest opiate receptor binding activity and beta-endorphin-like immunoreactivity were found in a fraction adsorbed on CM-cellulose.
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Department of Biochemistry, Chinese University of Hong Kong, Shatin, N.T.
D000313:Adrenal Medulla / Q000737:chemistry / Q000166:cytology; D000324:Adrenocorticotropic Hormone / Q000302:isolation & purification*; D000818:Animals; D007422:Intestines / Q000737:chemistry*; D009206:Myocardium / Q000737:chemistry*; D011863:Radioimmunoassay; D051381:Rats; D011957:Receptors, Opioid / Q000378:metabolism; D013256:Steroids / Q000096:biosynthesis; D014426:Turtles / Q000378:metabolism*; D015383:Zona Fasciculata / Q000737:chemistry / Q000166:cytology; D015385:Zona Reticularis / Q000737:chemistry / Q000166:cytology; D001615:beta-Endorphin / Q000302:isolation & purification*
D011957:Receptors, Opioid; D013256:Steroids; D001615:beta-Endorphin; D000324:Adrenocorticotropic Hormone