Electrophoresis of the complementary strands of the double-stranded Kemerovo virus RNAs in agarose-urea gel.
Single-stranded (ss)RNAs derived from 10 double-stranded (ds)RNA segments of Kemerovo virus (KV) were separated into 13 RNA bands by agarose-urea gel electrophoresis. The complementary strands of the dsRNA segments 1, 9 and 10 displayed different electrophoretic mobility. An attempt was made to determine the origin of the ssRNA bands. The ssRNA bands originating from the dsRNA segments 1, 2, 3, 9 and 10 were identified unequivocally, while those originating from the dsRNA segments 4, 5, 6, 7 and 8 were characterized partially. The minus RNA strands of the dsRNA segments 9 and 10 exhibited higher electrophoretic mobilities as their complementary plus RNA strands.
Bacík
I
I
Institute of Virology, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
D004587:Electrophoresis, Agar Gel; D018163:Orbivirus / Q000032:analysis*; D012330:RNA, Double-Stranded / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification*; D014508:Urea
D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014508:Urea