H(+)-ATPase, a primary pump for accumulation of neurotransmitters, is a major constituent of brain synaptic vesicles.
Upon treatment with sodium carbonate, rat brain synaptic vesicles lost ATP-dependent H+ transport and released major polypeptide components (about 72, 57, 41, 34 and 33 kDa). These polypeptides, consisting about 15% of the total protein, were identified as subunits of H(+)-ATPase by immunoblotting with antibodies against H(+)-ATPase from chromaffin granules. The same treatment also abolished the ATP-dependent, bafilomycin-sensitive uptakes of glutamate, serotonin and gamma-aminobutyrate by the synaptic vesicles. These results indicated that H(+)-ATPase is a major constituent of the vesicles (consisting about 20% of their total protein) and is a primary pump for accumulation of neurotransmitters.
Moriyama
Y
Y
Department of Organic Chemistry and Biochemistry, Osaka University, Japan.
Futai
M
M
D000818:Animals; D001692:Biological Transport; D001921:Brain / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D018377:Neurotransmitter Agents / Q000378:metabolism*; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D051381:Rats; D012701:Serotonin / Q000378:metabolism; D013572:Synaptic Vesicles / Q000201:enzymology* / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D018377:Neurotransmitter Agents; D012701:Serotonin; D005680:gamma-Aminobutyric Acid; D006180:Proton-Translocating ATPases