Defective brush-border expression of intrinsic factor-cobalamin receptor in canine inherited intestinal cobalamin malabsorption.
Ligand binding activity of intrinsic factor-cobalamin receptor (IFCR) was determined in homogenates and isolated brush-border membranes (BBM) of ileum and kidney from dogs exhibiting simple autosomal recessive inheritance of selective cobalamin malabsorption (Fyfe, J. C., Giger, U., Hall, C. A., Jezyk, P. F., Klumpp, S. A., Levine, J. S., and Patterson, D. F. (1991) Pediatr. Res. 29, 24-31). IFCR activity of affected dog ileal homogenates was 3-4-fold higher than normal whereas IFCR activity in affected dog kidney homogenates was one-tenth of normal. The recovery of IFCR activity in the BBM of ileum and renal cortex of affected dogs was 30- and 20-fold less than normal, respectively. The dissociation constant (Kd) for intrinsic factor-cobalamin was similar in BBM of both tissues and was the same in affected and normal dogs. In the affected dog ileal BBM, activities of alkaline phosphatase and sucrase-isomaltase and vesicular transport of glucose and Na(+)-taurocholate were normal. Immunoblots showed no IFCR cross-reactive material in the ileal or renal BBM of affected dogs. IFCR purified by affinity chromatography from kidney of both normal and affected dogs had an Mr = 230,000. However, amino acid analysis revealed that the affected dog IFCR had more lysine than the normal, and protease cleavage of the purified IFCRs revealed different peptide maps. Asparagine-linked oligosaccharides of both proteins were sensitive to peptide N-glycosidase F cleavage, but only the affected dog IFCR was endoglycosidase H sensitive. These results suggest that cobalamin malabsorption in this canine family is caused by inefficient BBM expression of IFCR due to a mutation of IFCR and its retention in an early biosynthetic compartment.
Fyfe
J C
JC
Section of Medical Genetics, Veterinary School, University of Pennsylvania, Philadelphia 19104-6010.
Ramanujam
K S
KS
Ramaswamy
K
K
Patterson
D F
DF
Seetharam
B
B
D000469:Alkaline Phosphatase / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D015153:Blotting, Western; D002451:Cell Compartmentation; D004283:Dog Diseases / Q000235:genetics* / Q000378:metabolism; D004285:Dogs; D005947:Glucose / Q000378:metabolism; D007082:Ileum / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D007437:Intrinsic Factor / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D008286:Malabsorption Syndromes / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*; D008871:Microvilli / Q000201:enzymology / Q000378:metabolism*; D010449:Peptide Mapping; D012964:Sodium / Q000502:physiology; D013393:Sucrase / Q000378:metabolism; D013656:Taurocholic Acid / Q000378:metabolism
D000596:Amino Acids; D013656:Taurocholic Acid; D007437:Intrinsic Factor; D012964:Sodium; D000469:Alkaline Phosphatase; D013393:Sucrase; D005947:Glucose