Pirenzepine block of ACH-induced mucus secretion in tracheal submucosal gland cells.
Muscarinic stimulation of mucus secretion, as measured by the release of [3H]glycoprotein, was studied in explants from the tracheal epithelium of weanling swine. The mucus glycoprotein secretion was transient, ceasing within the first 10 min of a continuous exposure to 100 microM ACh. Increasing the solution's osmotic pressure did not alter basal mucus glycoprotein secretion. Mucus glycoprotein secretion was inhibited by 2-10 microM PZP, indicating that the M3 muscarinic receptors mediate cholinergic stimulation of mucus production.
Farley
J M
JM
Department of Physiology University of Mississippi Medical Center, Jackson 39216-4505.
Dwyer
T M
TM
D000818:Animals; D046508:Culture Techniques; D006023:Glycoproteins / Q000378:metabolism; D008297:Male; D009093:Mucus / Q000378:metabolism*; D010890:Pirenzepine / Q000494:pharmacology*; D013552:Swine; D014132:Trachea / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
D006023:Glycoproteins; D010890:Pirenzepine