A procedure for cryopreservation of hamster oocytes yielding highly conserved oocytes suitable for sperm penetration tests.
Hamsters were superovulated and their oocytes frozen in Dulbecco's phosphate-buffered saline containing 10% fetal calf serum and 1.5 molar 1,2-propanediol. In several experiments, 94% of the hamster oocytes survived freezing and were equivalent to fresh oocytes in sperm penetration. In routine use of the procedure described, also 94% of 1,340 frozen-thawed oocytes were satisfactory for sperm penetration of several species. Frozen oocytes eliminate problems and costs of maintaining hamster colonies with variable responses on individual days.
Tobback
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Department of Animal Science, Cornell University Ithaca, New York 14853-4801.
Hough
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Foote
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D000704:Analysis of Variance; D000818:Animals; D006224:Cricetinae; D015925:Cryopreservation / Q000379:methods; D005260:Female; D005307:Fertilization in Vitro; D006064:Gonadotropins, Equine / Q000494:pharmacology; D008297:Male; D009865:Oocytes / Q000166:cytology* / Q000502:physiology; D013084:Sperm-Ovum Interactions; D013480:Superovulation
D006064:Gonadotropins, Equine