Sulfated polysaccharides prevent human leukocyte elastase-induced acute lung injury and emphysema in hamsters.
Studies were designed to explore the possibility that sulfated polysaccharides had the potential to prevent human leukocyte elastase (HLE)-induced lung injury. Arteparon (GAGPS), heparin, heparan sulfate, chondroitin sulfate, and dextran sulfate, but not dextran, inhibited HLE-mediated hydrolysis of succinyl-ala2-val-pNA. GAGPS, used as a paradigmatic sulfated polysaccharide, was a potent inhibitor of elastolysis in vitro. GAGPS given intratracheally prevented acute injury and emphysema in hamsters when administered up to 8 h before HLE insufflation. The results suggest that sulfated polysaccharides may be potent inhibitors of HLE-mediated lung injury.
Rao
N V
NV
Division of Pulmonary Medicine, University of Tennessee, Memphis.
Kennedy
T P
TP
Rao
G
G
Ky
N
N
Hoidal
J R
JR
D000818:Animals; D006224:Cricetinae; D005260:Female; D006025:Glycosaminoglycans / Q000494:pharmacology; D019272:Leukocyte Elastase; D008168:Lung / Q000187:drug effects; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors* / Q000633:toxicity; D011134:Polysaccharides / Q000494:pharmacology*; D011656:Pulmonary Emphysema / Q000209:etiology / Q000517:prevention & control*
D006025:Glycosaminoglycans; D011134:Polysaccharides; C010034:Arteparon; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase