Cloning and location of a gene governing lysine epsilon-aminotransferase, an enzyme initiating beta-lactam biosynthesis in Streptomyces spp.
In actinomycetes that produce beta-lactam antibiotics of the cephem type, lysine epsilon-aminotransferase is the initial enzyme in the conversion of lysine to alpha-aminoadipic acid. We used a two-stage process ("chromosome walking") to screen a lambda library of Streptomyces clavuligerus genomic DNA for fragments that expressed lysine epsilon-aminotransferase activity in S. lividans. Restriction analysis of the cloned DNA confirmed the location of the putative lat gene within the cluster of beta-lactam biosynthesis genes, roughly midway between pcbC, the structural gene for isopenicillin N synthetase, and the putative cefE gene encoding deacetoxycephalosporin C synthetase.
Madduri
K
K
Department of Microbiology, Dalhousie University, Halifax, Nova Scotia, Canada.
Stuttard
C
C
Vining
L C
LC
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D015139:Blotting, Southern; D016386:Chromosome Walking; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D051306:L-Lysine 6-Transaminase; D005810:Multigene Family; D015183:Restriction Mapping; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*; D000637:Transaminases / Q000235:genetics* / Q000378:metabolism; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D000637:Transaminases; D051306:L-Lysine 6-Transaminase