Binding of a nuclear factor to the upstream region of the c-myc gene.
We have studied the binding of proteins to a synthetic DNA fragment corresponding to a 21 base pair sequence in the upstream region of the human c-myc gene. This sequence has previously been reported to bind the c-myc protein, thus representing a putative site for autoregulation of the c-myc gene (Ariga et al., 1989). Our electrophoretic mobility shift assays indicated that this DNA fragment binds to a factor present in nuclear extracts from HeLa cells. However, analysis of extracts from other cells indicated that this binding does not correlate with the presence of the c-myc protein. No binding was observed using c-myc protein translated in vitro. Also, anti-c-myc antibodies could not inhibit binding of this factor. These experiments indicate, that nucleotides -2193 to -2173 from the P1 promoter of the c-myc gene are able to bind a nuclear factor, and that this factor most likely does not include the c-myc protein.
Saksela
K
K
Department of Virology, University of Helsinki, Finland.
Koskinen
P J
PJ
Alitalo
K
K
D015153:Blotting, Western; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D016259:Genes, myc / Q000502:physiology*; D006801:Humans; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
D016271:Proto-Oncogene Proteins c-myc; D014157:Transcription Factors