title: Isolation of a new gene (SW A2) encoding an alpha-amylase from Schwanniomyces occidentalis and its expression in Saccharomyces cerevisiae.
abstract: A new gene (SW A2) encoding a secretory alpha-amylase activity from Schwanniomyces occidentalis has been cloned from this yeast and then expressed in Saccharomyces cerevisiae. Both Sw, occidentalis and a transformant of S. cerevisiae incorporating SW A2 contain a transcript of 2.1 kb which hybridizes to DNa carrying the SW A2 gene. This indicates that the transcript is a product of the SW A2 gene. Transcription of the SW A2 gene seems to be regulated in both Sw. occidentalis and S. cerevisiae. Furthermore, the secretion of alpha-amylase activity is drastically repressed by glucose in both Sw. occidentalis and a transformant of S. cerevisiae containing SW A2.
authors.affiliation: Centro de Biologia Molecular (CSIC-UAM), Universidad Autónoma, Spain.
authors.forename: D
authors.initials: D
authors.lastname: Abarca
authors.forename: M
authors.initials: M
authors.lastname: Fernández-Lobato
authors.forename: L
authors.initials: L
authors.lastname: del Pozo
authors.forename: A
authors.initials: A
authors.lastname: Jiménez
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D010957:Plasmids; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D004718:Saccharomycetales / Q000201:enzymology*; D014158:Transcription, Genetic; D000516:alpha-Amylases / Q000235:genetics*
chemical_list: D000516:alpha-Amylases