The protein kinase inhibitor, K-252a, decreases elicitor-induced Ca2+ uptake and K+ release, and increases coumarin synthesis in parsley cells.
An elicitor preparation from fungal cell walls known to induce coumarin synthesis in suspension-cultured parsley cells also elicits a rapid and transient Ca2+ uptake, K+ release and external alkalinization, and increases uptake of 45Ca2+ into the cells. The latter three responses were inhibited by the protein kinase inhibitor K-252a at 0.2 microM. Elicitor-induced coumarin synthesis, a process which requires gene activation, was greatly enhanced by K-252a. These results suggest that protein phosphorylation might be involved in the initial steps of signal transduction as well as in the long-term induction of coumarin synthesis.
Conrath
U
U
FB Biologie, Universität Kaiserslautern, Germany.
Jeblick
W
W
Kauss
H
H
D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D002227:Carbazoles / Q000494:pharmacology*; D002478:Cells, Cultured; D003374:Coumarins / Q000378:metabolism*; D026121:Indole Alkaloids; D010944:Plants / Q000378:metabolism; D011188:Potassium / Q000378:metabolism*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*
D002227:Carbazoles; D003374:Coumarins; D026121:Indole Alkaloids; C049985:staurosporine aglycone; C030123:coumarin; D011493:Protein Kinase C; D011188:Potassium; D002118:Calcium