Rapid microscopic detection of malaria parasites permanently fluorochrome stained in blood smears with aluminum and morin.
Intra- and extracellular Plasmodium parasites in fixed blood smears are easily identifiable by fluorescence microscopy after brief mordanting with aluminum ammonium sulfate and staining with morin (3,5,7,1',4'-pentahydroxyflavanol). The intensely fluorescent preparations of stained parasites are strongly resistant to photodegradation and remained essentially unimpaired for two years.
Malinin
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GI
Department of Physics, Georgetown University, Washington, DC.
Malinin
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TI
D000535:Aluminum; D001801:Blood Stains; D005419:Flavonoids; D005456:Fluorescent Dyes; D006801:Humans; D007202:Indicators and Reagents; D008288:Malaria / Q000097:blood / Q000469:parasitology*; D008856:Microscopy, Fluorescence; D013997:Time Factors; D047309:Flavones
D000535:Aluminum; D005419:Flavonoids; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; C008548:morin; D047309:Flavones