Characterization of mucin antigens recognized by monoclonal antibodies raised against human colon cancer cells.
Two monoclonal antibodies, MLS 102, which recognizes cancer-associated mucin antigens, and MLS 103, which recognizes normal mucin, were used to isolate, by immunoaffinity chromatography, the corresponding antigens from cell lysates and spent medium of a human colorectal carcinoma cell line, LS 180. The MLS 102 antigen contained serine, threonine, and proline as major amino acids. The carbohydrate chains of the MLS 102 antigen were composed of O-linked NeuAc alpha 2----6GalNAc (56%), N-acetylgalactosamine (25%), and longer oligosaccharide chains. The MLS 103 antigen differed from the MLS 102 antigen in both amino acid and carbohydrate composition. Most O-linked oligosaccharides of the MLS 103 antigen were longer than the disaccharide found in the MLS 102 antigen. Immunostaining of LS 180 cells using MLS 102 and MLS 103 revealed that the cells are heterogeneous with respect to the expression of the antigens.
Fukui
S
S
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Horie
T
T
Numata
Y
Y
Kitagawa
H
H
Nakada
H
H
Kawasaki
T
T
Funakoshi
I
I
Yamashina
I
I
D000596:Amino Acids / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000912:Antibodies, Neoplasm / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000737:chemistry / Q000276:immunology*; D015179:Colorectal Neoplasms / Q000737:chemistry / Q000276:immunology*; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D006595:Hexosamines / Q000032:analysis; D006801:Humans; D015394:Molecular Structure; D008970:Molecular Weight; D009077:Mucins / Q000737:chemistry / Q000276:immunology*; D009844:Oligosaccharides / Q000737:chemistry; D014407:Tumor Cells, Cultured
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D006595:Hexosamines; D009077:Mucins; D009844:Oligosaccharides