Anti-triiodothyronine autoantibodies in a euthyroid woman: confirmation of immunoglobulin G antibodies employing protein A column chromatography.
The presence of thyroid hormone autoantibodies was investigated in a 48-year-old non-goitrous woman with high levels of serum total T3 and free T3. Her other thyroid function tests were normal. The presence of thyroid hormone autoantibodies were assessed in serum, acid-charcoal treated serum, IgG fractions and in a purified IgG fraction. the IgG fraction was separated from serum by a protein A-Sepharose CL-4B column chromatography and purified by gel filtration chromatography using Sephacryl S 200. Sera from normal individuals were used as controls. The results showed that the increased serum total and free T3 levels were caused by the presence of anti-T3 IgG autoantibodies. The results also indicate that protein A column chromatography is useful as a screening method for gross qualitative analyses of thyroid hormone autoantibodies.
Sugenoya
A
A
Department of Surgery, Shinshu University School of Medicine, Matsumoto, Japan.
Mizuno
E
E
Haniuda
M
M
Fujimori
M
M
Masuda
H
H
Kasuga
Y
Y
Kobayashi
S
S
Iida
F
F
D001323:Autoantibodies / Q000276:immunology*; D002845:Chromatography / Q000379:methods; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology* / Q000302:isolation & purification; D007457:Iodine Radioisotopes; D008875:Middle Aged; D011863:Radioimmunoassay; D013961:Thyroid Gland / Q000276:immunology* / Q000378:metabolism; D013970:Thyronines / Q000097:blood; D014284:Triiodothyronine / Q000097:blood / Q000276:immunology*
D001323:Autoantibodies; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D013970:Thyronines; D014284:Triiodothyronine