Intracellular localization of recombinant vaccinia virus produced HIV antigens and their use for confirmation of HIV seropositivity.
Recombinant vaccinia strains vC5 and vE234L expressing the rp50 and rgp160/rgp120 recombinant proteins were used in immunoblot and immunofluorescence assays. No false reactions were found, although 30 sera giving false reactivity with gag or env encoded proteins in a commercial immunoblot assay were included into the test panel. We recommend the recombinant protein-based assay for confirmation and discrimination of HIV seropositivity.
Bukrinsky
M I
MI
D. I. Ivanovsky Institute of Virology, Moscow, U.S.S.R.
Vzorov
A N
AN
Bukrinskaya
A G
AG
D005189:False Positive Reactions; D015488:HIV Antigens / Q000235:genetics / Q000378:metabolism*; D006679:HIV Seropositivity / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D011995:Recombination, Genetic; D013347:Subcellular Fractions / Q000382:microbiology; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D014764:Viral Proteins / Q000276:immunology / Q000378:metabolism
D015488:HIV Antigens; D011994:Recombinant Proteins; D014764:Viral Proteins