title: Nucleotide sequence of RNA 2 of a Czechoslovakian isolate of red clover necrotic mosaic virus.
abstract: The complete nucleotide sequence (1448 nucleotides) of RNA 2 of a Czechoslovakian isolate TpM-34 of red clover necrotic mosaic virus (RCNMV-TpM-34) has been determined. The sequence contained one major open reading frame (ORF) with the potential to encode a protein of 326 amino acids (Mr 35755), designated P2. The nucleotide sequence of RNA 2 of RCNMV-TpM-34 and the previously published sequence of RNA 2 of an Australian isolate of the virus (RCNMV-Aus) were 83% identical and there was 80% amino acid sequence identity between the P2 proteins of these isolates. However the N-terminal two-thirds of the P2 proteins shared a higher degree of similarity than the C-terminal regions which were predicted to have a more flexible structure. An ORF in the 3' portion of RNA 2 of RCNMV-Aus, which could encode a protein of Mr 5000, was not present in RNA 2 of RCNMV-TpM-34. RNAs 1 and 2 of RCNMV-TpM-34 and RCNMV-Aus are bilaterally compatible.
authors.affiliation: Department of Biology, Imperial College of Science, Technology and Medicine, London, U.K.
authors.forename: T A
authors.initials: TA
authors.lastname: Osman
authors.forename: S J
authors.initials: SJ
authors.lastname: Miller
authors.forename: A C
authors.initials: AC
authors.lastname: Marriott
authors.forename: K W
authors.initials: KW
authors.lastname: Buck
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003604:Czechoslovakia; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics* / Q000302:isolation & purification; D016366:Open Reading Frames; D011487:Protein Conformation; D012367:RNA, Viral / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins