Shuttle cloning and nucleotide sequences of Helicobacter pylori genes responsible for urease activity.
Production of a potent urease has been described as a trait common to all Helicobacter pylori so far isolated from humans with gastritis as well as peptic ulceration. The detection of urease activity from genes cloned from H. pylori was made possible by use of a shuttle cosmid vector, allowing replication and movement of cloned DNA sequences in either Escherichia coli or Campylobacter jejuni. With this approach, we cloned a 44-kb portion of H. pylori chromosomal DNA which did not lead to urease activity when introduced into E. coli but permitted, although temporarily, biosynthesis of the urease when transferred by conjugation to C. jejuni. The recombinant cosmid (pILL585) expressing the urease phenotype was mapped and used to subclone an 8.1-kb fragment (pILL590) able to confer the same property to C. jejuni recipient strains. By a series of deletions and subclonings, the urease genes were localized to a 4.2-kb region of DNA and were sequenced by the dideoxy method. Four open reading frames were found, encoding polypeptides with predicted molecular weights of 26,500 (ureA), 61,600 (ureB), 49,200 (ureC), and 15,000 (ureD). The predicted UreA and UreB polypeptides correspond to the two structural subunits of the urease enzyme; they exhibit a high degree of homology with the three structural subunits of Proteus mirabilis (56% exact matches) as well as with the unique structural subunit of jack bean urease (55.5% exact matches). Although the UreD-predicted polypeptide has domains relevant to transmembrane proteins, no precise role could be attributed to this polypeptide or to the UreC polypeptide, which both mapped to a DNA sequence shown to be required to confer urease activity to a C. jejuni recipient strain.
Labigne
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Unité des Entérobactéries, INSERM U199, Institut Pasteur, Paris, France.
Cussac
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Courcoux
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D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D016123:Campylobacter jejuni / Q000235:genetics; D003001:Cloning, Molecular; D003360:Cosmids; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D007887:Fabaceae / Q000201:enzymology; D005798:Genes, Bacterial; D005822:Genetic Vectors; D016480:Helicobacter pylori / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010946:Plants, Medicinal; D011513:Proteus mirabilis / Q000201:enzymology; D015183:Restriction Mapping; D014510:Urease / Q000235:genetics*
D004269:DNA, Bacterial; D014510:Urease