Extended HLA haplotypes in multiplex families with insulin dependent diabetes mellitus in Tunisian population: HLA serological typing and RFLP analysis.
Haplotypes including HLA A, B, C, DR, and DQ were compared in a study population comprising 18 Tunisian multiplex families with diabetic children. Eighty haplotypes found in IDDM patients were compared with 148 haplotypes present in healthy family members. RFLP analysis showed that two DR subtypes were significantly more common in the diabetic haplotypes (DR4-DQw8: 82 per cent in IDDM members compared to 0 per cent in healthy members, p less than 0.001 and DR-Dw25: 56 per cent in IDDM patients compared to 16.7 per cent in healthy members, p less than 0.001) and these were in most cases found in haplotype combinations with HLA A2 B44 DR4 DRw53 and HLA A 24 B18 DR3 genes, respectively.
Jenhani
F
F
Immunology Laboratory, Charles Nicolle, Tunis, Tunisia.
Bardi
R
R
Ayed
K
K
Chammakhi
S
S
Jeddi
M
M
Labonne
M P
MP
Gebuhrer
L
L
Betuel
H
H
D003922:Diabetes Mellitus, Type 1 / Q000235:genetics* / Q000276:immunology; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006680:HLA Antigens / Q000235:genetics*; D006683:HLA-DQ Antigens / Q000235:genetics; D006239:Haplotypes; D012150:Polymorphism, Restriction Fragment Length; D014416:Tunisia
D006680:HLA Antigens; D006683:HLA-DQ Antigens; C109756:HLA-DQ8 antigen