Peritoneal macrophages from patients on CAPD show an increased capacity to secrete interleukin-1 beta during peritonitis.
The in vitro release of interleukin-1 beta (IL-1 beta) by peritoneal macrophages from CAPD patients was studied during 16 infection free periods (16 patients) and 13 episodes of peritonitis (10 patients) using an ELISA. Without exogeneous stimulation with LPS, peritoneal macrophages released the same amounts of IL-1 beta. irrespective if they were obtained during an infection free period (473 +/- 92 pg/ml 24h, means +/- SEM) or during peritonitis (324 +/- 125 pg/ml). However, in response to a dose of 5 micrograms/ml of LPS, peritoneal macrophages released significantly more (p less than 0.005) IL-1 beta during peritonitis (6155 +/- 1743 pg/ml). These findings show that during peritonitis, peritoneal macrophages are primed in vivo to release more IL-1 beta in vitro after stimulation with LPS, indicating that activation of peritoneal macrophages for IL-1 beta secretion occurs stepwise.
Fieren
M W
MW
Department of Internal Medicine (I), University Hospital Dijkzigt, Erasmus University Rotterdam.
van den Bemd
G J
GJ
Bonta
I L
IL
D001424:Bacterial Infections / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000276:immunology; D008264:Macrophages / Q000378:metabolism*; D010529:Peritoneal Cavity / Q000166:cytology; D010531:Peritoneal Dialysis, Continuous Ambulatory / Q000009:adverse effects*; D010538:Peritonitis / Q000276:immunology*
D007375:Interleukin-1; D008070:Lipopolysaccharides