title: Interaction of fibronectin with heparin in model extracellular matrices: role of arginine residues and sulfate groups.
abstract: The interaction of heparin with the NH2-terminal domain of human plasma fibronectin was studied by using matrix-driven translocation, an assay for the adhesion of extracellular macromolecules with cell or particle surfaces within artificial collagen matrices. Partial desulfation of heparin rendered it ineffective in competitively inhibiting the interaction of the fibronectin NH2-terminal domain with heparin-coated particles, suggesting a role for sulfate groups of heparin in the interaction. Analysis of the fibronectin domain in terms of its primary structure, its proposed organization into "type I modules", and its hydrophilic and flexible segments led to the identification of several arginine-containing sites of potential interaction with the sulfate groups of heparin. Modification of increasing numbers of arginine side chains with 1,2-cyclohexanedione under mild conditions eventually led to decreases in translocation-promoting activity, and of heparin binding capacity as measured in a gel-shift assay, but the major portions of these functions were retained even when the four most accessible arginines (attributed to sites in and adjacent to the large loops of the type I modules) were modified. With the modification of additional arginines (attributed to sites in the small loops), both functions were lost. The peptide Gly-Arg-Gly, corresponding to a repeated determinant at the tips of two small loops, inhibited translocation, but arginine alone did not. Cleavage of the large loops by CNBr also led to loss of translocation-promoting activity. The correspondence between the molecular determinants of matrix-driven translocation and those previously found for mesenchymal morphogenesis indicates the utility of this system in the analysis of adhesive interactions of biological importance.
authors.affiliation: Department of Cell Biology and Anatomy, New York Medical College, Valhalla 10595.
authors.forename: N S
authors.initials: NS
authors.lastname: Jaikaria
authors.forename: L
authors.initials: L
authors.lastname: Rosenfeld
authors.forename: M Y
authors.initials: MY
authors.lastname: Khan
authors.forename: I
authors.initials: I
authors.lastname: Danishefsky
authors.forename: S A
authors.initials: SA
authors.lastname: Newman
mesh_terms: D000595:Amino Acid Sequence; D001120:Arginine; D003488:Cyanogen Bromide; D005109:Extracellular Matrix / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D006493:Heparin / Q000378:metabolism*; D006801:Humans; D008954:Models, Biological; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis; D011485:Protein Binding; D011487:Protein Conformation; D012091:Repetitive Sequences, Nucleic Acid; D013464:Sulfuric Acids
chemical_list: D005353:Fibronectins; D009842:Oligopeptides; D013464:Sulfuric Acids; D006493:Heparin; D001120:Arginine; D003488:Cyanogen Bromide