title: Cross-linked hemoglobin solution as a resuscitative fluid after hemorrhage in the rat.
abstract: Intramolecularly (alpha-alpha) cross-linked hemoglobin has been reported to have oxygen transport properties similar to those of whole blood. The present study evaluated the efficacy of diaspirin alpha-alpha cross-linked hemoglobin solution as a resuscitation fluid, with heart rate, mean arterial pressure, and transcutaneous oxygen tension as the study parameters. Rats were bled and approximately one third of their total blood volume (20 ml/kg) was removed while they were anesthetized; they were then resuscitated with 14% hemoglobin solution. Animals that received either 10 mg/kg (n = 10) or 20 mg/kg (n = 10) of hemoglobin solution responded quickly and positively to the infusions: mean arterial pressure (which had dropped to less than 40% of prehemorrhage levels) returned to baseline within 2 minutes of initiating infusion; by 4 minutes, the mean arterial pressures of the hemoglobin-infused groups were significantly higher (p less than or equal to 0.05) than those in both the autologous shed blood (n = 8) and lactated Ringer's (n = 10) groups. The heart rate and transcutaneous oxygen tension responses in both the half-volume and full-volume replacement hemoglobin groups matched the response to autologous shed blood throughout the hour of observation. The favorable hemodynamic response to infusion of cross-linked hemoglobin solution after hemorrhage suggests that this material is comparable to autologous shed blood and superior to lactated Ringer's solution as a resuscitative fluid as assessed in this model.
authors.affiliation: Medical Research Fellowship Program, Walter Reed Army Institute of Research, Washington, D.C.
authors.forename: R J
authors.initials: RJ
authors.lastname: Przybelski
authors.forename: D S
authors.initials: DS
authors.lastname: Malcolm
authors.forename: D G
authors.initials: DG
authors.lastname: Burris
authors.forename: R M
authors.initials: RM
authors.lastname: Winslow
mesh_terms: D000818:Animals; D001785:Blood Gas Monitoring, Transcutaneous; D001794:Blood Pressure; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D006339:Heart Rate; D006400:Hematocrit; D006454:Hemoglobins / Q000627:therapeutic use*; D006470:Hemorrhage / Q000097:blood / Q000503:physiopathology / Q000628:therapy*; D007773:Lactates / Q000097:blood; D019344:Lactic Acid; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012151:Resuscitation / Q000379:methods*; D012996:Solutions
chemical_list: D003432:Cross-Linking Reagents; D006454:Hemoglobins; D007773:Lactates; D012996:Solutions; D019344:Lactic Acid