After X-irradiation a transient arrest of L929 cells in G2-phase coincides with a rapid elevation of the level of O6-alkylguanine-DNA alkyltransferase.
Following X-irradiation of exponentially growing L929 cells two major phenomena have been observed. First, there was a delay in cell division which can be ascribed to the arrest of cells in the G2-phase (G2-block), and, second, the cellular content of the O6-alkylguanine-DNA alkyltransferase (AGT) was markedly increased. Flow cytometrical DNA-measurements revealed that cells began to accumulate in the G2-phase 4 h after irradiation (p.r.) irrespective of the X-ray dose, while both the fraction of cells blocked in G2 and the time period the cells persisted in G2 increased with the radiation dose. About 24 h past release from the G2-block the distribution of cells in the cell cycle was similar to that of untreated control cells. In comparison with control cells the AGT content in irradiated cells (4 Gy) was highest at about 48 h p.r. (3.4-fold increase). The highest ratio of increase in AGT was, however, observed to occur between about 4 and 13 h p.r. (2.6-fold increase). As shown by flow cytometrical measurements using a BrdUrd/DNA double labeling technique, this rapid primary increase in AGT coincides very well with the entrance of cells into the G2-phase. This indicates that the cellular AGT content in X-irradiated (parental) cells started to exceed the basal level at the beginning of the G2-phase, but not before or during the S-phase. Once the AGT level was elevated it continued to increase for 2 to 3 cell doubling times.
Nehls
P
P
Universität Ulm, Abteilung für Molekularbiologie und Allgemeine Pathologie, Federal Republic of Germany.
van Beuningen
D
D
Karwowski
M
M
D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism / Q000528:radiation effects*; D002453:Cell Cycle / Q000528:radiation effects; D004261:DNA Replication / Q000528:radiation effects*; D029968:Escherichia coli Proteins; D005434:Flow Cytometry; D016195:G2 Phase / Q000528:radiation effects*; D007700:Kinetics; D007739:L Cells / Q000166:cytology / Q000201:enzymology / Q000528:radiation effects; D051379:Mice; D019853:O(6)-Methylguanine-DNA Methyltransferase; D014157:Transcription Factors; D014965:X-Rays
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D014157:Transcription Factors; C045675:Ada protein, E coli; D019853:O(6)-Methylguanine-DNA Methyltransferase