[Effects of alpha adrenergic stimulation on the mechanical properties of the myocardium].
The mechanical effects of phenylephrine at 2 x 10(-6) M (PE1, n = 8), 2 x 10(-5) M (PE2, n = 10) and 2 x 10(-4) M (PE3, n = 6) were studied on rat left ventricular papillary muscle, in the presence of propranolol (4 x 10(-7) M) and 0.5 mM of (Ca2+)e. The contraction-relaxation coupling was studied under isotonic and isometric conditions. The maximal velocity of contraction (max Vc) and relaxation (max Vr) were calculated during isotonic contraction with preload only at L max. The positive (+ dF/dt max) and negative (- dF/dt max) peaks of the derivative of the force were calculated during isometric contraction. Two coefficients, R1 = max Vc/max Vr and R2 (+ dF/dt max)/(- dF/dt max) provided an appreciation of the contraction-relaxation coupling at low and high loads respectively. The positive inotropic effect observed in the three groups was accompanied by a significant decrease of the coefficient R1 (PE1: - 11 +/- 2% p less than 0.001; PE2: - 15 +/- 2%, p less than 0.001; PE3: -20 +/- 2%, p less than 0.001). On the other hand, no significant variations of the coefficient R2 were observed (PE1: 3 +/- 3%; PE2: 1 +/- 4%; PE3: 5 +/- 3%). The proportionally greater improvement in the velocity of relaxation compared to the velocity of contraction at low loads suggests that the sarcoplasmic reticulum is involved in the expression of positive inotropic and positive lusitropic effects of alpha-adrenergic stimulation.
el Amrani
A I
AI
INSERM U 275, laboratoire d'optique appliquée, ENSTA-école polytechnique, Palaiseau.
Riou
B
B
Pourny
J C
JC
Lecarpentier
Y
Y
D000316:Adrenergic alpha-Agonists / Q000494:pharmacology*; D000818:Animals; D006801:Humans; D009200:Myocardial Contraction / Q000187:drug effects* / Q000502:physiology; D010656:Phenylephrine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000502:physiology; D013268:Stimulation, Chemical
D000316:Adrenergic alpha-Agonists; D010656:Phenylephrine