Identification of the K99 (F5) fimbrial adhesin in commercial vaccines used against calf enteritis.
A monoclonal antibody (Mab)-based antigen capture enzyme-linked immunosorbent assay (ELISA) was developed and standardised for the detection of the K99 adhesin of Escherichia coli in aqueous vaccines. A repeating epitope on the K99 antigen is first captured then detected using peroxidase-labelled antibody. The assay gave positive results with all the vaccines tested that were known to contain K99 and was specific, rapid and reproducible.
Thorns
C J
CJ
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, Weybridge, Surrey, U.K.
Sawtell
J A
JA
Miller
J A
JA
Wood
G W
GW
D018830:Adhesins, Escherichia coli; D000818:Animals; D000911:Antibodies, Monoclonal; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000592:standards; D001428:Bacterial Vaccines / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control; D004751:Enteritis / Q000517:prevention & control / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D004927:Escherichia coli Infections / Q000517:prevention & control / Q000662:veterinary; D012015:Reference Standards
D018830:Adhesins, Escherichia coli; D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines