title: A dominant idiotype in the antibody response against the influenza virus hemagglutinin. Serum and in situ analyses.
abstract: PY206 is an Id associated with a BALB/c murine mAb described as being specific for the influenza A virus hemagglutinin. However, production of this Id by BALB/c mice immunized with influenza is low. This report shows that the PY206 Id is a dominant component of the anti-influenza antibody response in C57BL/6J strain mice infected intranasally with the influenza A/Hong Kong/168/(H3N2)[R] X-31 virus. High PY206 Id expression was linked to the IgHb Ig allotype locus. PY206 Id+ antibody-forming cells were identified in situ in cryostat sections of lymphoid tissues and idiotypic heterogeneity was identified among PY206+ B cells. Uninfected adult C57BL/6J mice had PY206 Id in their serum that lacked influenza binding specificity. In situ analysis of prenatal and neonatal spleen of uninfected C57BL/6J mice showed that the expansion of PY206 Id+ B cells occurred early in development. PY206+ cells were demonstrated in the lungs of influenza-infected mice but not in normal mice, establishing the capability to study this B cell population in the lung. This model offers the opportunity to manipulate the anti-influenza A virus hemagglutinin B cell response and to study the proliferation and migration of influenza-specific B cells in their native tissue environments.
authors.affiliation: Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38101.
authors.forename: A R
authors.initials: AR
authors.lastname: Brown
authors.forename: R E
authors.initials: RE
authors.lastname: Sealy
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000097:blood; D001402:B-Lymphocytes / Q000276:immunology; D005333:Fetus / Q000276:immunology; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral / Q000276:immunology*; D015151:Immunoblotting; D007126:Immunoglobulin Allotypes / Q000502:physiology; D007130:Immunoglobulin Idiotypes / Q000096:biosynthesis*; D009980:Influenza A virus / Q000276:immunology*; D008168:Lung / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL
chemical_list: D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral; D007126:Immunoglobulin Allotypes; D007130:Immunoglobulin Idiotypes