The cloning and functional characterization of the nifH gene of Rhodospirillum rubrum.
Dinitrogenase reductase (the nifH product) from Rhodospirillum rubrum is regulated by a post-translational modification system encoded by draTG. As demonstrated in this report, the cloning, sequencing, and functional characterization of the nifH gene provides a basis for further analysis as well as revealing interesting features of gene organization. The coding regions of nifH and draT are separated by only 400 bp, though the genes are divergently transcribed and differentially regulated. The construction of a nifH insertion caused a Nif- phenotype and destroyed the mutant's ability to synthesize both dinitrogenase and dinitrogenase reductase, verifying functionality and transcriptional organization of the nifHDK genes.
Lehman
L J
LJ
Department of Bacteriology, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706.
Fitzmaurice
W P
WP
Roberts
G P
GP
D036002:ADP Ribose Transferases / Q000235:genetics; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D017037:Dinitrogenase Reductase; D005288:Ferredoxins / Q000235:genetics*; D005798:Genes, Bacterial; D006026:Glycoside Hydrolases / Q000235:genetics; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009699:N-Glycosyl Hydrolases; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D012247:Rhodospirillum rubrum / Q000235:genetics*
D017037:Dinitrogenase Reductase; D005288:Ferredoxins; D004247:DNA; D036002:ADP Ribose Transferases; C057637:dinitrogenase reductase ADP-ribosyltransferase; D006026:Glycoside Hydrolases; D009699:N-Glycosyl Hydrolases; C036091:ADP-ribosyl-(dinitrogen reductase) hydrolase