Dimerization of the P-glycoprotein in membranes.
Plasma membranes from a CHO cell line, CHRC5, which exhibits multidrug resistance was studied using radiation inactivation analysis. The P-glycoprotein content of the membrane was determined by Western blots. Irradiation resulted in the loss of P-glycoprotein. The dependence of this loss on radiation dose corresponded to a target size of 250 kDa which is the molecular mass of a dimer of the P-glycoprotein. This is strong evidence to indicate that the P-glycoprotein self associates in the membrane.
Boscoboinik
D
D
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada.
Debanne
M T
MT
Stafford
A R
AR
Jung
C Y
CY
Gupta
R S
RS
Epand
R M
RM
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism / Q000528:radiation effects*; D006224:Cricetinae; D004351:Drug Resistance; D004591:Electrophoresis, Polyacrylamide Gel; D008562:Membrane Glycoproteins / Q000378:metabolism* / Q000528:radiation effects; D013329:Structure-Activity Relationship
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D008562:Membrane Glycoproteins