Identification of 'cystic fibrosis protein' as a complex of two calcium-binding proteins present in human cells of myeloid origin.
Cystic fibrosis protein is a serum protein characterized by a pI close to 8.4 and present with a higher concentration in serum and plasma of cystic fibrosis carriers than in controls. This protein was found immunologically indistinguishable from the cystic fibrosis antigen isolated from granulocytes and presenting a sequence analogous to that of MRP-8, a calcium-binding protein expressed in the myeloid cell lineage. Using antibodies directed against MRP-8 and its closely associated calcium-binding protein, MRP-14, we demonstrate here that cystic fibrosis protein purified from serum is a complex of the two proteins MRP-8 and MRP-14.
Barthe
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C
Groupe de Recherche sur les Glandes Exocrines, Marseille, France.
Figarella
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Carrère
J
J
Guy-Crotte
O
O
D001798:Blood Proteins / Q000378:metabolism*; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D040501:Calgranulin A; D003550:Cystic Fibrosis / Q000378:metabolism*; D006098:Granulocytes / Q000378:metabolism; D006801:Humans; D046911:Macromolecular Substances
D001798:Blood Proteins; D002135:Calcium-Binding Proteins; D040501:Calgranulin A; D046911:Macromolecular Substances; C026422:cystic fibrosis serum factor