title: Effect of passaging on Mer phenotype of human fetal cell cultures.
abstract: With increasing passage in culture, the human fibroblast cell strain GM11 lost the Mer+ phenotype (the ability to support the growth of adenovirus 5 damaged prior to infection by MNNG). All of 46 embryonic strains prepared either from various organs of 20 fetuses from 6-7 to 13-14 weeks of gestational age or from two hydatidiform moles showed normal repair of MNNG-treated virus. We conclude that human fetal strains are not usually deficient in such repair, and that the behavior of GM11 is atypical.
authors.affiliation: Department of Medicine, Cross Cancer Institute, Edmonton, Alb., Canada.
authors.forename: R S
authors.initials: RS
authors.lastname: Day
authors.forename: L H
authors.initials: LH
authors.lastname: Honore
authors.forename: K D
authors.initials: KD
authors.lastname: Dobler
mesh_terms: D000260:Adenoviruses, Human / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D004260:DNA Repair; D005260:Female; D005333:Fetus; D006801:Humans; D006828:Hydatidiform Mole / Q000235:genetics; D066298:In Vitro Techniques; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009154:Mutation; D011247:Pregnancy; D014594:Uterine Neoplasms / Q000235:genetics
chemical_list: D008769:Methylnitronitrosoguanidine