Interleukin 3-dependent proliferation of the human Mo-7e cell line is supported by discrete activation of late G1 genes.
The hemopoietic growth factor interleukin 3 (IL-3) supports the survival and proliferation of multipotent and committed progenitor cells in vitro. To elucidate the molecular mechanisms triggered by IL-3 we studied the expression of cell cycle-related genes in a recently established human IL-3-dependent clone (M-07e). No changes in the level of expression of early (c-myc), mid (ornithine decarboxylase), or mid-late G1 (p53, c-myb) cell cycle genes were detected after restoration of IL-3 in deprived cells. The fact that only late G1-S-phase genes [proliferating cell nuclear antigen (PCNA) thymidine kinase (TK), histone H3] are modulated by IL-3 suggests that this factor may control human cell proliferation by acting at the G1-S boundary.
Avanzi
G C
GC
Dipartimento di Scienze Biomediche ed Oncologia Umana, Università degli studi di Torino, Italy.
Porcu
P
P
Brizzi
M F
MF
Ghigo
D
D
Bosia
A
A
Pegoraro
L
L
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D016193:G1 Phase; D005786:Gene Expression Regulation; D016259:Genes, myc; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology*; D009955:Ornithine Decarboxylase / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis
D007377:Interleukin-3; D012333:RNA, Messenger; D004247:DNA; D009955:Ornithine Decarboxylase