title: Ductal lesions of exocrine glands and insulitis induced by L3T4+ T cells following graft-versus-host reaction due to major histocompatibility complex class II disparity.
abstract: Recently, we have demonstrated characteristic hepatic lesions resembling primary biliary cirrhosis (PBC) in semiallogeneic F1 hybrid mice with major histocompatibility complex (MHC) class II-disparate graft-versus-host reaction (GVHR). In the present study, we tried to reveal other ductal lesions in extrahepatic organs, including salivary glands and pancreas. Murine strains used are C57BL/6 (B6), B6 mutant bm1, and bm12. bm1 carries a mutant gene at the H-2K locus of MHC and bm12 carries a mutant gene at the I-A locus of MHC of the B6 strain. The (B6 x bm1)F1, (B6 x bm12)F1, and (bm1 x bm12)F1 mice were injected intravenously with 1 x 10(7) B6 L3T4+ or Lyt-2+ T cells and were sacrificed on the 14th day postinjection for histological examinations. Mononuclear cell infiltration was detected around the ducts of salivary glands only in (B6 x bm12)F1 mice injected with B6 L3T4+ T cells. A moderate to marked level of cell infiltration was demonstrated in pancreas of (B6 x bm12)F1 recipients similar to nonobese diabetic (NOD) mice. By immunohistochemical examinations, infiltrating cells were shown to consist not only of L3T4+ but also of Lyt-2+ T cells, even after the inoculation of L3T4+ cells. These results are discussed in reference to mechanisms of organ-specific autoimmune diseases, especially insulitis in NOD mice which show insulin-dependent diabetes mellitus.
authors.affiliation: Department of Immunology, Niigata University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Saitoh
authors.forename: M
authors.initials: M
authors.lastname: Fujiwara
authors.forename: H
authors.initials: H
authors.lastname: Asakura
mesh_terms: D000818:Animals; D000921:Antibody-Producing Cells / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D005088:Exocrine Glands / Q000473:pathology*; D006086:Graft vs Host Disease / Q000150:complications* / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007515:Islets of Langerhans / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D010183:Pancreatic Ducts / Q000473:pathology; D010195:Pancreatitis / Q000209:etiology*; D012469:Salivary Glands / Q000473:pathology; D013154:Spleen / Q000378:metabolism
chemical_list: D000949:Histocompatibility Antigens Class II; D007074:Immunoglobulin G; D007075:Immunoglobulin M