Measurement of protein synthesis by the phenylalanine flooding dose technique: effect of phenylalanine and anaesthesia on plasma electrolyte, enzyme and metabolite levels.
Ten minutes after an intravenous flooding dose of phenylalanine to rats, plasma sodium and calcium concentrations were slightly reduced (by 2-7%) but no effects on potassium or phosphate were observed. Creatine kinase activities were significantly increased by phenylalanine injection (by 39%), but alkaline phosphatase, alanine aminotransferase, lactate dehydrogenase and aspartate aminotransferase activities were unaltered. Plasma concentrations of total proteins, albumin, cholesterol, triglycerides, urea, creatinine and glucose were also unaffected. In the presence of anaesthesia, phenylalanine injection had almost identical effects, although the increase in creatine kinase activities did not reach statistical significance. Anaesthesia for 10 min reduced plasma potassium concentrations (by 27%), and calcium (by 5%), though phosphate and sodium were unaltered. The activities of lactate dehydrogenase, creatine kinase and aspartate aminotransferase were reduced by between 36-52%, but alkaline phosphatase and alanine aminotransferase activities were unaltered by anaesthesia. Plasma concentrations of total proteins and albumin were also reduced (both by 9%), but glucose concentrations were increased (by 33%). Anaesthesia had no other significant effects on cholesterol, triglycerides, urea or creatinine concentrations. The qualitative effects of anaesthesia in the presence of raised free phenylalanine concentrations were similar. It was concluded that, except for creatine kinase, determinations of plasma constituents in phenylalanine-injected rats could be made without overt interpretational errors. However, caution is required in interpreting data on plasma constituents from anaesthetized rats.
Preedy
V R
VR
Department of Clinical Biochemistry, King's College School of Medicine and Dentistry, London, UK.
Hammond
B
B
Bottiglieri
T G
TG
Marway
J S
JS
Peters
T J
TJ
D000410:Alanine Transaminase / Q000097:blood*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood*; D001769:Blood / Q000187:drug effects* / Q000378:metabolism; D001798:Blood Proteins / Q000096:biosynthesis*; D002118:Calcium / Q000097:blood*; D003402:Creatine Kinase / Q000378:metabolism*; D004347:Drug Interactions; D007275:Injections, Intravenous; D008297:Male; D008733:Methoxyflurane / Q000494:pharmacology*; D010649:Phenylalanine / Q000494:pharmacology*; D051381:Rats; D012964:Sodium / Q000097:blood*
D001798:Blood Proteins; D008733:Methoxyflurane; D010649:Phenylalanine; D012964:Sodium; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D003402:Creatine Kinase; D002118:Calcium