title: Effect of dietary alpha-linolenic acid on equine monocyte procoagulant activity and eicosanoid synthesis.
abstract: To investigate the effects of an omega-3 fatty acid-enriched ration on the in vitro response of equine monocytes to endotoxin, an 8-week feeding trial was conducted in which linseed oil served as the source of the omega-3 fatty acid, alpha-linolenic acid. One group of horses was fed a control pelleted ration and the other group was fed an 8% linseed oil-enriched pelleted ration. After 8 weeks of feeding, monocytes were isolated and incubated in the presence of Escherichia coli O55:B5 endotoxin for 6 hr. After 8 weeks on the rations, the mean procoagulant activity and thromboxane B2 production by endotoxin-stimulated monocytes from horses consuming the linseed oil ration decreased by 51% and 71%, respectively, compared with cells from horses consuming the control ration. There was no difference in monocyte synthesis of 12-hydroxyeicosatetraenoic acid or leukotriene B4 between groups. Fatty acid analysis of membrane phospholipids revealed a decrease in the omega-6:omega-3 ratio in monocytes from horses consuming the linseed oil ration. These data suggest that dietary supplementation with alpha-linolenic acid may modify the response to endotoxin by reducing the synthesis of potentially harmful cellular mediators.
authors.affiliation: Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602.
authors.forename: M M
authors.initials: MM
authors.lastname: Henry
authors.forename: J N
authors.initials: JN
authors.lastname: Moore
authors.forename: E B
authors.initials: EB
authors.lastname: Feldman
authors.forename: J K
authors.initials: JK
authors.lastname: Fischer
authors.forename: B
authors.initials: B
authors.lastname: Russell
mesh_terms: D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000818:Animals; D001777:Blood Coagulation; D000001:Calcimycin / Q000494:pharmacology; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D015777:Eicosanoids / Q000096:biosynthesis*; D004731:Endotoxins / Q000494:pharmacology; D004926:Escherichia coli; D005227:Fatty Acids / Q000097:blood; D005260:Female; D006736:Horses / Q000097:blood*; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis; D007975:Leukotriene B4 / Q000096:biosynthesis; D008042:Linolenic Acids / Q000494:pharmacology*; D008043:Linseed Oil / Q000494:pharmacology; D008297:Male; D008563:Membrane Lipids / Q000097:blood; D009000:Monocytes / Q000187:drug effects / Q000502:physiology*; D010743:Phospholipids / Q000097:blood; D013929:Thromboxane B2 / Q000096:biosynthesis; D017962:alpha-Linolenic Acid
chemical_list: D004042:Dietary Fats, Unsaturated; D015777:Eicosanoids; D004731:Endotoxins; D005227:Fatty Acids; D006893:Hydroxyeicosatetraenoic Acids; D008042:Linolenic Acids; D008563:Membrane Lipids; D010743:Phospholipids; D017962:alpha-Linolenic Acid; D007975:Leukotriene B4; D000001:Calcimycin; D013929:Thromboxane B2; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D008043:Linseed Oil