Glutathione-dependent and ketone body-related enzymes in the selenium-deficient rat.
Male rats, born of selenium (Se)-depleted dams and continuously fed a Se-deficient diet regimen or Se-repleted for 11 weeks, were used for some enzymatic determinations in samples from liver, heart, kidney and hind limb muscle. The Se-dependent GSH-Px activity in liver and kidney of the Se-deficient group was less than 0.5% of Se-repleted controls, whereas a 3-fold increase in liver and kidney GSH S-transferase activity was found in these Se-deficient rats. The Se-deficient group excreted 3 times more acetoacetate via the urine than their Se-repleted siblings. The activity of the key enzymes of ketone body utilization was similar in tissues from Se-repleted and Se-deficient rats. It is suggested that longlasting or even Se-unresponsive changes in enzymes of ketone body metabolism might have developed in these rats born of Se-depleted dams.
Olsson
U
U
Department of Genetic and Cellular Toxicology, Wallenberg Laboratory, University of Stockholm, Sweden.
D000090:Acetoacetates / Q000652:urine; D000101:Acetyl-CoA C-Acetyltransferase / Q000378:metabolism*; D000123:Acetyltransferases / Q000378:metabolism*; D000818:Animals; D015256:Coenzyme A-Transferases / Q000378:metabolism*; D005260:Female; D005978:Glutathione / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007657:Ketone Bodies / Q000378:metabolism*; D008297:Male; D009928:Organ Specificity; D051381:Rats; D011919:Rats, Inbred Strains; D012643:Selenium / Q000172:deficiency*
D000090:Acetoacetates; D007657:Ketone Bodies; C016635:acetoacetic acid; D005979:Glutathione Peroxidase; D000123:Acetyltransferases; D000101:Acetyl-CoA C-Acetyltransferase; D005982:Glutathione Transferase; D015256:Coenzyme A-Transferases; C021460:3-ketoacid CoA-transferase; D005978:Glutathione; D012643:Selenium