Fission-neutron-induced expression of a tumour-associated antigen in human cell hybrids (HeLa x skin fibroblasts): evidence for increased expression at low dose rate.
The induction of a tumour-associated antigen in a human cell hybrid line (HeLa x skin fibroblast) following exposure to fission neutrons of average energy 0.85 MeV (Janus reactor, Argonne National Laboratory) at two dose rates, 0.086 and 10.3 cGy/min, has been examined. The dose-response data obtained indicate the lower dose rate to be 2.9-fold more effective than the higher in inducing expression of the tumour-associated antigen, while there was no significant dose-rate effect in terms of cell killing. These results are qualitatively in agreement with previous observations using neutrons from the Janus reactor for the neoplastic transformation of C3H10T1/2 cells and Syrian hamster embryo cells.
Redpath
J L
JL
Department of Radiological Sciences, University of California, Irvine 92715.
Hill
C K
CK
Jones
C A
CA
Sun
C
C
D000951:Antigens, Neoplasm / Q000096:biosynthesis*; D002470:Cell Survival / Q000528:radiation effects; D002471:Cell Transformation, Neoplastic / Q000276:immunology / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells / Q000528:radiation effects*; D066298:In Vitro Techniques; D009502:Neutrons; D012867:Skin / Q000166:cytology
D000951:Antigens, Neoplasm