Identification of a brain-specific 27/26-kDa extracellular protein with the monoclonal antibody to differentiated PC12h pheochromocytoma cells.
We have searched for brain-specific extracellular molecules using a library of monoclonal antibodies against surface antigens of differentiated PC12h cells. One of the monoclonal antibodies, PCH42-14, recognized a 27/26-kDa protein of 10-week-old rat brain on immunoblotting. PCH42-14 antigen was detected only in brain, especially in cerebrum, olfactory bulb, mesencephalon, hippocampus, medulla oblongata, and spinal cord. On hippocampal neuron culture, PCH42-14 antigen existed extracellularly along with the neuronal extensions.
Ishikawa
M
M
Department of Biology, Ochanomizu University, Tokyo, Japan.
Kuroda
Y
Y
Kobayashi
K
K
Sawada
H
H
Hayashi
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D001923:Brain Chemistry; D015151:Immunoblotting; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009474:Neurons / Q000737:chemistry*; D009928:Organ Specificity; D010673:Pheochromocytoma; D051381:Rats; D011919:Rats, Inbred Strains; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D009419:Nerve Tissue Proteins