Induction of growth arrest by a temperature-sensitive p53 mutant is correlated with increased nuclear localization and decreased stability of the protein.
A temperature-sensitive mutant of p53, p53Val-135, was found to be able to arrest cell proliferation when overexpressed at 32.5 degrees C. While much of the protein was cytoplasmic in cells proliferating at 37.5 degrees C, it became predominantly nuclear at 32.5 degrees C. Concomitantly, p53Val-135 became destabilized, although not to the extent seen in primary fibroblasts.
Ginsberg
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Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
Michael-Michalovitz
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Ginsberg
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Oren
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M
D000818:Animals; D002451:Cell Compartmentation; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D005455:Fluorescent Antibody Technique; D066298:In Vitro Techniques; D051379:Mice; D009154:Mutation; D013696:Temperature; D016159:Tumor Suppressor Protein p53 / Q000502:physiology*
D016159:Tumor Suppressor Protein p53