title: The same CCAAT box-binding factor binds to the promoter of two coordinately regulated major histocompatibility complex class II genes.
abstract: Using competition mobility shift, methylation interference, and proteolytic clipping DNA binding assays, we demonstrate that the protein binding the major histocompatibility complex A beta CCAAT box is indistinguishable from the protein previously named NF-Y, which binds the major histocompatibility complex E alpha CCAAT box. Although the two CCAAT boxes share the same 10-base core sequence, termed the Y box, their flanking sequences, known to be important for binding, are very different.
authors.affiliation: Department of Microbiology and Immunology, Albert B Chandler Medical Center, University of Kentucky, Lexington 40536-0084.
authors.forename: M J
authors.initials: MJ
authors.lastname: Kern
authors.forename: J G
authors.initials: JG
authors.lastname: Woodward
mesh_terms: D000818:Animals; D001483:Base Sequence; D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins / Q000378:metabolism*; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D066298:In Vitro Techniques; D008285:Major Histocompatibility Complex; D051379:Mice; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D000949:Histocompatibility Antigens Class II; D009841:Oligonucleotides; D014157:Transcription Factors