title: Cellular mechanisms of somatostatin action in the gut.
abstract: We have used isolated canine parietal cells to examine the receptor and postreceptor events mediating the inhibitory effects of somatostatin on acid secretion. Somatostatin-14 (S14) and somatostatin-28 (S28) dose dependently inhibited parietal cells stimulated by secretagogues that activate both the adenylate cyclase/cyclic adenosine monophosphate and the inositol phospholipid/protein kinase C cascades. The inhibitory action was mediated via a specific cell surface receptor that consists of a single subunit protein (molecular weight 99,000 d). This receptor recognized S14 and S28 equally well. Somatostatin inhibited parietal cell activity via mechanisms that are both dependent on and independent of a pertussis toxin-sensitive inhibitory guanine nucleotide binding protein.
authors.affiliation: Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109-0362.
authors.forename: J
authors.initials: J
authors.lastname: DelValle
authors.forename: J
authors.initials: J
authors.lastname: Park
authors.forename: T
authors.initials: T
authors.lastname: Chiba
authors.forename: T
authors.initials: T
authors.lastname: Yamada
mesh_terms: D000818:Animals; D004285:Dogs; D019204:GTP-Binding Proteins / Q000494:pharmacology; D005744:Gastric Acid / Q000378:metabolism*; D066298:In Vitro Techniques; D010295:Parietal Cells, Gastric / Q000187:drug effects* / Q000378:metabolism; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000502:physiology; D017481:Receptors, Somatostatin; D015398:Signal Transduction / Q000187:drug effects; D013004:Somatostatin / Q000494:pharmacology*; D053561:Somatostatin-28
chemical_list: D017981:Receptors, Neurotransmitter; D017481:Receptors, Somatostatin; D013004:Somatostatin; D053561:Somatostatin-28; D019204:GTP-Binding Proteins