Accumulation and metabolism of Ins(1,4,5)P3 and Ins(1,3,4,5)P4 in muscarinic-receptor-stimulated SH-SY5Y neuroblastoma cells.
Stimulation of M3 muscarinic receptors expressed by SH-SY5Y cells induced a dose- and time-related increase in the mass of Ins(1,4,5)P3 (basal 38.3 +/- 5.8 pmol/mg of protein) and Ins(1,3,4,5)P4 (basal 6.1 +/- 1.2 pmol/mg of protein). Comparison of radioreceptor mass assays with [3H]inositol labelling showed higher-fold stimulations with the former protocol. The later accumulation of Ins(1,4,5)P3 and Ins(1,3,4,5)P4 mass was dependent upon extracellular Ca2+.
Lambert
D G
DG
Department of Pharmacology and Therapeutics, University of Leicester, U.K.
Challiss
R A
RA
Nahorski
S R
SR
D000818:Animals; D002217:Carbachol / Q000494:pharmacology*; D002460:Cell Line; D007294:Inositol / Q000378:metabolism; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D007295:Inositol Phosphates / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D009447:Neuroblastoma; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000502:physiology*
D007295:Inositol Phosphates; D011976:Receptors, Muscarinic; C081655:inositol-1,3,4,5-tetrakisphosphate; D007294:Inositol; D015544:Inositol 1,4,5-Trisphosphate; D002217:Carbachol