Nucleotide sequence analysis of the 3' half of the genome of bovine leukaemia virus grown in FLK cells.
The DNA sequence of the env and px (tat) regions of bovine leukaemia virus cloned from integrated proviral DNA of the virus producing FLK cell line was determined and compared with published sequences cloned from bovine leukaemia virus-induced tumours of cattle. The homology of 97% between a Belgian tumour clone and the FLK clone was significantly lower than that between a Japanese tumour clone and the FLK clone, where less than 1% nucleotide exchanges were observed. The sequences of cDNAs synthesized from purified virus RNA material which had been grown in different FLK sublines were found completely identical with one another as well as with the equivalent FLK proviral DNA sequences.
Rosenthal
S
S
Centre for Molecular Biology, Academy of Sciences of G.D.R., Berlin-Buch.
Drescher
B
B
Weber
S
S
Möhring
R
R
D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D003062:Codon; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D015752:Genes, env; D015756:Genes, tat; D001909:Leukemia Virus, Bovine / Q000235:genetics*; D009154:Mutation; D009690:Nucleic Acid Conformation; D012091:Repetitive Sequences, Nucleic Acid; D012190:Retroviridae / Q000235:genetics*; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D003062:Codon; D004279:DNA, Viral; D015682:Retroviridae Proteins, Oncogenic