Formation of Z,E,E-geranylgeranyl diphosphate by rat liver microsomes.
Rat liver microsomes catalyzed the formation of A,E,E-geranylgeranyl diphosphate from farnesyl diphosphate and isopentenyl diphosphate in the presence of Triton X-100. Studies on product specificity using various primers such as Z,E-farnesyl diphosphate, E,E-farnesyl diphosphate, Z,E,E-geranylgeranyl diphosphate, E,E,E-geranylgeranyl diphosphate, Z,E,E,E-geranylfarnesyl diphosphate, and E,E,E,E-geranylfarnesyl diphosphate suggested that the microsomal dehydrodolichyl diphosphate synthase has such properties that it releases Z,E,E-geranylgeranyl diphosphate, the first intermediate, in the reactions with farnesyl diphosphate as the starting primer. Metabolic labeling of rat liver slices with [2-3H]mevalonic acid revealed the accumulation of E,E,E-geranylgeranyl (di)phosphates as well as dolichyl (di)phosphate (C85 and C90) and dehydrodolichol (C85 and C90), but no accumulation of Z,E,E-geranylgeranyl (di)phosphate or E,E-farnesyl (di)phosphate was detected. Microsomal enzyme preparations from mouse liver and hamster liver also produced Z,E,E-geranylgeranyl diphosphate from farnesyl diphosphate and isopentenyl diphosphate.
Sagami
H
H
Chemical Research Institute of Non-Aqueous Solutions, Tohoku University, Sendai, Japan.
Matsuoka
S
S
Ogura
K
K
D019883:Alkyl and Aryl Transferases; D000818:Animals; D002384:Catalysis; D002855:Chromatography, Thin Layer; D006224:Cricetinae; D008297:Male; D008647:Mesocricetus; D051379:Mice; D008807:Mice, Inbred BALB C; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism*; D011106:Polyisoprenyl Phosphates / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity; D014166:Transferases / Q000378:metabolism
D011106:Polyisoprenyl Phosphates; D014166:Transferases; D019883:Alkyl and Aryl Transferases; C051389:dehydrodolichyl diphosphate synthetase; C002963:geranylgeranyl pyrophosphate