Somatostatin inhibition of Ca2(+)-induced insulin secretion in permeabilized HIT-T15 cells.
Somatostatin inhibited Ca2(+)-induced insulin secretion in permeabilized HIT-T15 cells, albeit with decreased sensitivity relative to intact cells. The inhibitory action required the presence of GTP, whereas GDP could not substitute for GTP. Pertussis-toxin treatment before cell permeabilization abolished the inhibition of secretion. Thus somatostatin, by activating a G-protein, interferes with exocytosis distal to the generation of soluble intracellular messengers.
Ullrich
S
S
Division de Biochimie Clinique, Centre Médical Universitaire, Geneva, Switzerland.
Prentki
M
M
Wollheim
C B
CB
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002460:Cell Line; D002463:Cell Membrane Permeability; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D019204:GTP-Binding Proteins / Q000502:physiology; D005947:Glucose / Q000494:pharmacology; D006150:Guanine Nucleotides / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007930:Leucine / Q000494:pharmacology; D037342:Pertussis Toxin; D012636:Secretory Rate / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects; D013004:Somatostatin / Q000494:pharmacology*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D006150:Guanine Nucleotides; D007328:Insulin; D010566:Virulence Factors, Bordetella; D013004:Somatostatin; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins; D007930:Leucine; D005947:Glucose; D002118:Calcium