title: GABAA and GABAB antagonists prevent the opioid inhibition of endogenous acetylcholine release evoked by glutamate from rat neostriatal slices.
abstract: We have examined the role of the GABAergic system in the opioid inhibition of endogenous acetylcholine (ACh) release from rat neostriatal slices by blocking either gamma-aminobutyric acid-A (GABAA) or GABAB receptors. GABAergic antagonists (bicuculline or phaclofen) completely blocked mu- (morphine or DAGO) and delta-opioid (DPDPE) inhibition of glutamate-evoked endogenous ACh release in a concentration-dependent manner. However, GABA antagonists were ineffective in blocking the opioid inhibition of potassium-evoked endogenous ACh release. These findings point to the important role of the GABAergic system in the regulation of mu- and delta-opioid inhibition of cholinergic neurons stimulated by glutamate.
authors.affiliation: Departament de Biologia Cellular i Anatomia Patològica, Facultat de Medicina, Universitat de Barcelona, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Arenas
authors.forename: J
authors.initials: J
authors.lastname: Marsal
authors.forename: J
authors.initials: J
authors.lastname: Alberch
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D001418:Baclofen / Q000031:analogs & derivatives* / Q000494:pharmacology; D001640:Bicuculline / Q000494:pharmacology*; D003342:Corpus Striatum / Q000187:drug effects / Q000502:physiology*; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; D020881:Enkephalin, D-Penicillamine (2,5)-; D004745:Enkephalins / Q000494:pharmacology; D018756:GABA Antagonists; D005971:Glutamates / Q000494:pharmacology*; D018698:Glutamic Acid; D066298:In Vitro Techniques; D008297:Male; D009020:Morphine / Q000494:pharmacology; D009294:Narcotics / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D011963:Receptors, GABA-A / Q000187:drug effects / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
chemical_list: D004745:Enkephalins; D018756:GABA Antagonists; D005971:Glutamates; D009294:Narcotics; D011963:Receptors, GABA-A; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; C052329:phaclofen; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D009020:Morphine; D020881:Enkephalin, D-Penicillamine (2,5)-; D001418:Baclofen; D000109:Acetylcholine; D001640:Bicuculline