title: Network antibodies identify nuclear lamin B as a physiological attachment site for peripherin intermediate filaments.
abstract: We studied the molecular associations between peripherin (a neuronal, type III intermediate filament subunit) and nuclear lamins. We show here that isolated peripherin binds selectively to mammalian lamin B under in vitro conditions. We further demonstrate that a synthetic peptide, representing the proximal part of peripherin's tail domain (P1), also associates with mammalian lamin B in a saturable, cooperative, and specific fashion. Laboratory animals immunized with P1 spontaneously develop idiotypic and anti-idiotypic antibodies recognizing peripherin and lamin B, respectively. These data provide essentially in vivo evidence that lamin B represents a constitutive nuclear "receptor" site for the tail domains of peripherin intermediate filaments.
authors.affiliation: College de France, Biochimie Cellulaire, Paris.
authors.forename: K
authors.initials: K
authors.lastname: Djabali
authors.forename: M M
authors.initials: MM
authors.lastname: Portier
authors.forename: F
authors.initials: F
authors.lastname: Gros
authors.forename: G
authors.initials: G
authors.lastname: Blobel
authors.forename: S D
authors.initials: SD
authors.lastname: Georgatos
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000502:physiology / Q000648:ultrastructure*; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D004625:Embryo, Nonmammalian; D004912:Erythrocytes / Q000502:physiology / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D007381:Intermediate Filament Proteins / Q000235:genetics / Q000378:metabolism*; D007382:Intermediate Filaments / Q000502:physiology / Q000648:ultrastructure*; D007700:Kinetics; D034921:Lamin Type B; D034882:Lamins; D008562:Membrane Glycoproteins; D051379:Mice; D008862:Microsomes, Liver / Q000502:physiology / Q000648:ultrastructure*; D008930:Mitochondria, Liver / Q000502:physiology / Q000648:ultrastructure*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D009447:Neuroblastoma; D009474:Neurons / Q000502:physiology / Q000648:ultrastructure*; D009479:Neuropeptides / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D064531:Peripherins; D011485:Protein Binding; D051381:Rats; D015183:Restriction Mapping; D014422:Turkeys
chemical_list: D007381:Intermediate Filament Proteins; D034921:Lamin Type B; D034882:Lamins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D009687:Nuclear Proteins; D064531:Peripherins