title: Microglial progenitors with a high proliferative potential in the embryonic and adult mouse brain.
abstract: Single cell suspensions, prepared from brain stem, cerebellum, and forebrain parenchyma of embryonic and adult mice, were plated on monolayers of an astroglial cell line derived from a spontaneously immortalized mouse cerebellar culture, the D19 clone. A few of the brain cells adhering to the D19 monolayers were immunoreactive to the Mac-1 antibody, which labels all cells of the monocytic and granulocytic lineages. The Mac-1-positive cells proliferated vigorously and later most of them acquired the F4/80 epitope specific for macrophages and microglia cells. Studies in clonal conditions allowed development of large colonies of about 2 x 10(5) cells that expressed typical microglia markers. Bone marrow Mac-1-positive cells cocultured on D19 monolayers were also induced to proliferate, whereas peritoneal macrophages were not. D19 astrocytes express macrophage colony-stimulating factor (CSF-1) activity at a high level, and their conditioned media induced the proliferation of brain and bone marrow Mac-1-positive cells. A specific anti-CSF-1 antiserum completely blocked bone marrow macrophage progenitor proliferation and significantly reduced the multiplication of microglial precursors induced by the D19-conditioned medium. These data indicate that the embryonic and adult mouse brain parenchyma contains potential progenitors for microglial cells.
authors.affiliation: Centre de Biologie Cellulaire, Centre National de la Recherche Scientifique, Ivry sur Seine, France.
authors.forename: F
authors.initials: F
authors.lastname: Alliot
authors.forename: E
authors.initials: E
authors.lastname: Lecain
authors.forename: B
authors.initials: B
authors.lastname: Grima
authors.forename: B
authors.initials: B
authors.lastname: Pessac
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001253:Astrocytes / Q000166:cytology / Q000502:physiology; D001921:Brain / Q000166:cytology* / Q000196:embryology; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D006412:Hematopoietic Stem Cells / Q000166:cytology; D007150:Immunohistochemistry; D016173:Macrophage Colony-Stimulating Factor / Q000032:analysis; D016177:Macrophage-1 Antigen / Q000032:analysis; D008648:Mesoderm / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D009457:Neuroglia / Q000166:cytology*
chemical_list: D000911:Antibodies, Monoclonal; D016177:Macrophage-1 Antigen; D016173:Macrophage Colony-Stimulating Factor