title: Induction of sperm head abnormalities by incorporated radionuclides: dependence on subcellular distribution, type of radiation, dose rate, and presence of radioprotectors.
abstract: In contrast to the biological effects caused by exposure to external beams of radiation, the effects of tissue-incorporated radionuclides are highly dependent on the type of radiation emitted and on their distribution at the macroscopic, microscopic, and subcellular levels, which are in turn determined by the chemical nature of the radionuclides administered. Induction of abnormalities of sperm heads in mice is investigated in this work after the injection of a variety of radiochemicals including alpha emitters. When the initial slopes of the dose-response curves are used to compare the relative biological effectiveness (RBE) of different radiocompounds, the alpha particles emitted in the decay of 210Po are more effective than Auger electrons emitted by 125I incorporated in the DNA of the spermatogonial cells, and both emissions are more effective than X rays. It is also shown that the Auger emitters (125I, 111In) distributed in the cell nucleus are more efficient in producing abnormalities than the same radionuclides localized in the cytoplasm. These findings are consistent with our earlier observations, where spermatogonial cell survival is assayed as a function of the testicular absorbed dose. Further, chronic irradiation of testis with gamma rays from intratesticularly administered 7Be is about three times more effective in causing abnormalities than a single acute exposure to 120-kVp X rays. The resulting RBE values correlate well with our data on sperm head survival with the same radiocompounds. Finally, the radioprotector cysteamine, when administered in small, nontoxic amounts, significantly reduces the incidence of sperm abnormalities from alpha-particle radiation as well as emissions from 125I incorporated into DNA, the dose reduction factors being 10 and 14, respectively.
authors.affiliation: Department of Radiology, University of Medicine & Dentistry of New Jersey, Newark 07103.
authors.forename: D V
authors.initials: DV
authors.lastname: Rao
authors.forename: V R
authors.initials: VR
authors.lastname: Narra
authors.forename: R W
authors.initials: RW
authors.lastname: Howell
authors.forename: V K
authors.initials: VK
authors.lastname: Lanka
authors.forename: K S
authors.initials: KS
authors.lastname: Sastry
mesh_terms: D000512:Alpha Particles; D000818:Animals; D001608:Beryllium / Q000008:administration & dosage; D003543:Cysteamine / Q000008:administration & dosage / Q000627:therapeutic use; D004583:Electrons; D005720:Gamma Rays; D007205:Indium Radioisotopes / Q000008:administration & dosage; D007267:Injections; D007457:Iodine Radioisotopes / Q000008:administration & dosage; D008297:Male; D051379:Mice; D011059:Polonium / Q000008:administration & dosage; D011837:Radiation-Protective Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D011868:Radioisotopes / Q000008:administration & dosage*; D012062:Relative Biological Effectiveness; D013077:Sperm Head / Q000528:radiation effects*; D013737:Testis
chemical_list: D007205:Indium Radioisotopes; D007457:Iodine Radioisotopes; D011837:Radiation-Protective Agents; D011868:Radioisotopes; D003543:Cysteamine; D011059:Polonium; D001608:Beryllium