cDNA and deduced amino acid sequences of a dog liver cytochrome P-450 of the IIIA gene subfamily.
A 1.96 kbp cDNA encoding a male Beagle dog liver cytochrome P-450 of 503 amino acid residues (Mr 57,636) has been isolated and sequenced. The deduced amino acid sequence is 79.8%, 69.3% and 74.1% identical to the P450IIIA forms human NF25, rat PCN1 and rabbit LM3c, respectively. The amino terminal sequence is identical to the first 28 residues of the dog P450IIIA form PBD-1. Southern blot analysis yields restriction patterns consistent with IIIA gene subfamily multiplicity.
Ciaccio
P J
PJ
Department of Pharmacology, Fox Chase Cancer Center, Philadelphia, PA 19111.
Graves
P E
PE
Bourque
D P
DP
Glinsmann-Gibson
B
B
Halpert
J R
JR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004285:Dogs; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D005810:Multigene Family; D011817:Rabbits; D012689:Sequence Homology, Nucleic Acid
D004247:DNA; D003577:Cytochrome P-450 Enzyme System