Assignment of an erbB-like DNA sequence to linkage group VI in fishes of the genus Xiphophorus (Poeciliidae).
The inheritance of 23 protein polymorphisms was compared with the inheritance of a DNA restriction fragment length polymorphism (RFLP) of a strongly cross-hybridizing erbB-related sequence, epidermal growth factor receptor-like-1 (EGFRL1), in Xiphophorus clemenciae X X. milleri-derived backcross hybrids. Two polymorphic bands were noted in this cross with a v-erbB probe after PstI digestion: a 10-kilobase (kb) band in X. clemenciae and a 9-kb band in X. milleri. Joint segregation analysis of the RFLPs and protein polymorphisms indicate that this erbB-related sequence can be assigned to Xiphophorus linkage group VI, which also comprises genes coding for glutamine synthetase (GLNS), nucleoside phosphorylase-2 (NP2), and transferin (TF). We have designated this RFLP as alleles at a locus called EGFRL1 because of very strong cross-hybridization with the v-erbB probe, known to be homologous to the mammalian EGFR gene. This mapping assignment is the first autosomal linkage of an oncogene sequence reported in fish, which provide a large number of genetically controlled experimental tumor models.
Harless
J
J
University of Texas M.D. Anderson Cancer Center, Science Park-Research Division, Smithville 78957.
Svensson
R
R
Kallman
K D
KD
Morizot
D C
DC
Nairn
R S
RS
D000818:Animals; D015139:Blotting, Southern; D003198:Computer Simulation; D003532:Cyprinodontiformes / Q000235:genetics*; D015342:DNA Probes; D004592:Electrophoresis, Starch Gel; D066246:ErbB Receptors / Q000235:genetics*; D008040:Genetic Linkage; D009857:Oncogenes; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D015342:DNA Probes; D066246:ErbB Receptors