title: Interaction of nitrofurans with glutathione reductase.
abstract: Nitrofurans inhibit the oxidation of NADPH by glutathione, catalyzed by yeast glutathione reductase (EC 1.6.4.2). acting as uncompetitive incomplete inhibitors for NADPH and glutathione. The quinoline-substituted nitrofurans were the most effective inhibitors. These compounds increased the turnover numbers of enzyme at fixed concentrations of reduced glutathione, in the reverse reaction of glutathione reductase, but in most cases diminished the affinity of the enzyme for NAD+. Nitrofurans are weak one-electron oxidants of glutathione reductase. Their reactivity is close to that of p-quinones possessing the analoguous one-electron reduction potential (Cénas, N.K., Rakauskiené, G.A. and Kulys, J.J. (1989) Biochim. Biophys. Acta 973, 399-404), and reaction is stimulated by NADP+. It is assumed, that nitrofurans bind to the 'regulative' site of glutathione reductase (Karplus, P.A., Pai, E.F. and Schulz, G.E. (1989) Eur. J. Biochem. 178, 693-703).
authors.affiliation: Institute of Biochemistry, Lithuanian Academy of Sciences, Vilnius, U.S.S.R.
authors.forename: N K
authors.initials: NK
authors.lastname: Cénas
authors.forename: D A
authors.initials: DA
authors.lastname: Bironaité
authors.forename: J J
authors.initials: JJ
authors.lastname: Kulys
authors.forename: N M
authors.initials: NM
authors.lastname: Sikhova
mesh_terms: D005978:Glutathione / Q000378:metabolism; D005980:Glutathione Reductase / Q000037:antagonists & inhibitors* / Q000737:chemistry; D066298:In Vitro Techniques; D007700:Kinetics; D009249:NADP / Q000378:metabolism; D009581:Nitrofurans / Q000737:chemistry / Q000494:pharmacology*; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae / Q000201:enzymology
chemical_list: D009581:Nitrofurans; D009249:NADP; D005980:Glutathione Reductase; D005978:Glutathione