title: Ha-ras-1 alleles in Norwegian lung cancer patients.
abstract: We have examined DNA restriction fragment length polymorphisms (RFLP) of the Ha-ras-1 gene in DNA from 118 lung cancer patients and 123 unaffected controls. When DNA samples were digested with MspI/HpaII restriction endonucleases. Southern blot analysis demonstrated 4 common, 4 intermediate and 7 different rare alleles in the combined population after hybridization to the pGDa1 probe. Six of the rare alleles were unique for the lung cancer group and 1 rare allele for the control group. The frequency of rare alleles in lung cancer patients (10/236) was significantly different (P less than 0.01) from the control group (1/246). The lung cancer group also had a significantly lower frequency of the common 2.57 kb fragment than the controls (P less than 0.02). The results thus indicate that Ha-ras genotyping may be of value in lung cancer risk assessment.
authors.affiliation: Department of Toxicology, National Institute of Occupational Health, Oslo, Norway.
authors.forename: D
authors.initials: D
authors.lastname: Ryberg
authors.forename: T
authors.initials: T
authors.lastname: Tefre
authors.forename: S
authors.initials: S
authors.lastname: Ovrebø
authors.forename: V
authors.initials: V
authors.lastname: Skaug
authors.forename: L
authors.initials: L
authors.lastname: Stangeland
authors.forename: A
authors.initials: A
authors.lastname: Naalsund
authors.forename: R
authors.initials: R
authors.lastname: Baera
authors.forename: A L
authors.initials: AL
authors.lastname: Børresen
authors.forename: A
authors.initials: A
authors.lastname: Haugen
mesh_terms: D000483:Alleles; D015139:Blotting, Southern; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics; D011905:Genes, ras; D005838:Genotype; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D015342:DNA Probes; D004273:DNA, Neoplasm