Saccharomyces cerevisiae transcription factors IIIB and IIIC bend the DNA of a tRNA(Gln) gene.
The ability of Saccharomyces cerevisiae transcription factors IIIC and IIIB to bend DNA at their binding sites in a tRNA(Gln) gene has been analyzed by two methods that are based on the anomalous electrophoretic mobility of bent DNA in a tight gel network. Both transcription factors bend DNA, TFIIIB more than TFIIIC. The effective centers of the transcription factor IIIB- and IIIC-induced bends are not in helical phase.
Léveillard
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Department of Biology, University of California, San Diego, La Jolla 92093.
Kassavetis
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Geiduschek
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D001345:Autoradiography; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000235:genetics; D012351:RNA, Transfer, Gln / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D035361:Transcription Factor TFIIIB; D014157:Transcription Factors / Q000378:metabolism*; D019474:Transcription Factors, TFIII; D000098832:Transcription Factor TFIIIC
D005656:Fungal Proteins; D012351:RNA, Transfer, Gln; D035361:Transcription Factor TFIIIB; D014157:Transcription Factors; D019474:Transcription Factors, TFIII; D000098832:Transcription Factor TFIIIC; D004247:DNA