Structural proteins of hog cholera virus expressed by vaccinia virus: further characterization and induction of protective immunity.
A cDNA fragment covering the genomic region that encodes the structural proteins of hog cholera virus (HCV) was inserted into the tk gene of vaccinia virus. Expression studies with vaccinia virus/HCV recombinants led to identification of HCV-specific proteins. The putative HCV core protein p23 was demonstrated for the first time by using an antiserum against a bacterial fusion protein. The glycoproteins expressed by vaccinia virus/HCV recombinant migrated on sodium dodecyl sulfate-gels identically to glycoproteins precipitated from HCV-infected cells. A disulfide-linked heterodimer between gp55 and gp33 previously detected in HCV-infected cells was also demonstrated after infection with the recombinant virus. The vaccinia virus system allowed us to identify, in addition to the heterodimer, a disulfide-linked homodimer of HCV gp55. The vaccinia virus/HCV recombinant that expressed all four structural proteins induced virus-neutralizing antibodies in mice and swine. After immunization of pigs with this recombinant virus, full protection against a lethal challenge with HCV was achieved. A construct that lacked most of the HCV gp55 gene failed to induce neutralizing antibodies but induced protective immunity.
Rümenapf
T
T
Federal Research Centre for Virus Diseases of Animals, Tübingen, Federal Republic of Germany.
Stark
R
R
Meyers
G
G
Thiel
H J
HJ
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D002872:Chromosome Deletion; D006692:Classical Swine Fever Virus / Q000235:genetics* / Q000276:immunology; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005814:Genes, Viral; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology; D007106:Immune Sera; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009500:Neutralization Tests; D010957:Plasmids; D011995:Recombination, Genetic; D015183:Restriction Mapping; D014616:Vaccinia virus / Q000235:genetics* / Q000276:immunology; D015678:Viral Structural Proteins / Q000235:genetics* / Q000276:immunology
D004279:DNA, Viral; D006023:Glycoproteins; D007106:Immune Sera; D015678:Viral Structural Proteins