Cloning and mapping of the potato virus Y genome and its in vitro expression.
Full-length cDNA of genomic RNA of potato virus Y (PVY) was cloned in one piece into a lambda vector. The order of the EcoRI and SalI fragments of the inserted cDNA was determined. This is the first report of the cloning of a long, expressible, potyvirus genome. The availability of such a clone is a prerequisite for any further study of the molecular biology of this group of viruses, as they are expressed into a self-processed primary polyprotein.
Karchi
M
M
Virus Laboratory, Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel.
Sela
I
I
Edelbaum
O
O
Ori
A
A
Raccah
B
B
Rosner
A
A
Stram
Y
Y
D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004587:Electrophoresis, Agar Gel; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010942:Plant Viruses / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011233:Precipitin Tests; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D012689:Sequence Homology, Nucleic Acid