title: Yeast proteins with reactivity to antibodies elicited against mammalian apolipoproteins.
abstract: Hyperimmunized rabbit anti-rat apolipoprotein antibodies showed reactivity to proteins in Saccharomyces cerevisiae. Antiapolipoproteins A1, B and E reacted with proteins in both a crude extract and a lipid enriched fraction. Protein reactivity was dependent on antisera dilution. Furthermore, the different antiapolipoproteins tested reacted with individually distinct yeast proteins, possibly suggesting the presence of a variety of yeast apolipoproteins with distinct cellular functions as is the case with mammalian apolipoproteins. The specificity of the antibody was directed toward the yeast protein and not a lipid moiety.
authors.affiliation: Department of Microbiology, North Carolina State University, Raleigh.
authors.forename: G A
authors.initials: GA
authors.lastname: Keesler
authors.forename: S
authors.initials: S
authors.lastname: Moore
authors.forename: D C
authors.initials: DC
authors.lastname: Usher
authors.forename: L W
authors.initials: LW
authors.lastname: Parks
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000032:analysis; D001053:Apolipoproteins / Q000097:blood / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000276:immunology* / Q000302:isolation & purification; D015151:Immunoblotting; D011817:Rabbits / Q000276:immunology; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000276:immunology*
chemical_list: D000906:Antibodies; D000936:Antigen-Antibody Complex; D001053:Apolipoproteins; D005656:Fungal Proteins