A clonal CD4-positive T-cell line established from the blood of a patient with Sézary syndrome.
The reported inability to establish long-term T-cell lines from the blood of cutaneous T-cell lymphoma patients with circulating neoplastic T cells has hindered the development of an in vitro system to investigate Sézary syndrome. We have established a rapidly proliferating T-cell line from the peripheral blood of a patient with Sézary syndrome, which expresses a mature helper T-cell phenotype and contains cytogenetic abnormalities and T-cell receptor gene rearrangements identical to those in the patient's blood. The method of establishment and characteristics of this line are described.
Abrams
J T
JT
Wistar Institute, Philadelphia, PA 19104.
Lessin
S
S
Ghosh
S K
SK
Ju
W
W
Vonderheid
E C
EC
Nowell
P
P
Murphy
G
G
Elfenbein
B
B
DeFreitas
E
E
D000368:Aged; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis*; D002460:Cell Line; D046508:Culture Techniques / Q000379:methods; D015342:DNA Probes; D004261:DNA Replication / Q000187:drug effects; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation; D010641:Phenotype; D012751:Sezary Syndrome / Q000276:immunology*; D012878:Skin Neoplasms / Q000276:immunology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface; D015704:CD4 Antigens; D015342:DNA Probes; D004273:DNA, Neoplasm; D007376:Interleukin-2