Results of the First International Granulocyte Serology Workshop.
Eleven laboratories participated in the First International Workshop of Granulocyte Serology. Participants were asked to identify any granulocyte-reactive antibodies present in 12 human sera distributed as unknown samples. Granulocyte immunofluorescence and granulocyte micro-agglutination were the most commonly used techniques. The incidence of false-positive and false-negative reactions was 15 and 4%, respectively. Most laboratories were able to distinguish between granulocyte-specific and anti-HLA antibodies using platelet absorption. The identification of the specificity of granulocyte-specific antibodies caused the most problems; these were correctly identified on only 57% of occasions.
Lucas
G F
GF
Blood Group Reference Laboratory, South Western Regional Blood Transfusion Centre, Bristol, UK.
Carrington
P A
PA
D000918:Antibody Specificity / Q000276:immunology; D006098:Granulocytes / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007518:Isoantibodies / Q000032:analysis; D010641:Phenotype; D011237:Predictive Value of Tests; D012699:Serology / Q000379:methods*
D006680:HLA Antigens; D007518:Isoantibodies