Large-scale purification of membrane-containing bacteriophage PRD1 and its subviral particles.
PRD1 is a dsDNA virus that infects Escherichia coli and Salmonella typhimurium. The genome is a linear molecule with 5' covalently linked terminal protein. The virus has a lipid membrane inside the protein coat. We describe the large-scale purification of the virus using a zonal rotor and the yields and quality of the virus and its subviral assemblies for subsequent biophysical measurements.
Bamford
J K
JK
Department of Genetics, University of Helsinki, Finland.
Bamford
D H
DH
D002501:Centrifugation, Zonal; D004279:DNA, Viral / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D012483:Salmonella Phages / Q000302:isolation & purification*; D012486:Salmonella typhimurium
D004279:DNA, Viral