Limited repertoire of the C-terminal region of the M protein in Streptococcus pyogenes.
We have amplified genomic sequences (emm) that may encode M protein from strains of Streptococcus pyogenes using the polymerase chain reaction (PCR). Genomic DNA from 22 isolates representing 14 M serotypes was selected for the study. Primers which corresponded to the observed N-terminal signal sequence and the variable C-terminal sequences of emm6, emm49 and ennX were used. PCR products using emm6 and emm49 oligonucleotides were classified into two mutually exclusive groups which correspond to the presence or absence of serum opacity factor. These findings support the concept of limited heterogeneity in the C-terminal sequences of the M protein.
Relf
W A
WA
Menzies School of Health Research, Casuarina, Australia.
Sriprakash
K S
KS
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D002352:Carrier Proteins; D004587:Electrophoresis, Agar Gel; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013297:Streptococcus pyogenes / Q000235:genetics*
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; C014689:streptococcal M protein