Characterization of cucumber mosaic virus RNA-dependent RNA polymerase.
Cucumber mosaic virus (CMV) RNA-dependent RNA polymerase (RdRp) was purified form CMV-infected tobacco. The purified enzyme is completely dependent on exogenous template. The enzyme utilizes a variety of viral RNAs and CMV satellite RNA as template for minus-strand synthesis. Cellular RNAs are not used as templates. Ribosomal RNA inhibits the viral RNA synthesis by the CMV RdRp.
Quadt
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Department of Biochemistry, Gorlaeus Laboratories, Leiden Unviversity, The Netherlands.
Jaspars
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EM
D009029:Mosaic Viruses / Q000201:enzymology*; D010944:Plants / Q000382:microbiology; D012367:RNA, Viral / Q000378:metabolism; D012324:RNA-Dependent RNA Polymerase / Q000302:isolation & purification* / Q000378:metabolism; D013379:Substrate Specificity; D013698:Templates, Genetic
D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase