Effect of antipsychotic drugs on the molecular action of cholera toxin in rabbit intestinal epithelial cells.
Antipsychotic drugs of known antidiarrhoeal and anticalmodulin activity inhibited the cholera-toxin-catalysed ADP-ribosylation of proteins of Mr 37,000, 40,000 and 45,000 (thought to be regulatory components of the adenylate cyclase complex) that was previously shown to occur in plasma membranes from rabbit intestinal epithelial cells [(1989) Biochim. Biophys. Acta 1014, 289-297]. There was no obvious correlation between the different activities of the drugs. The drugs also inhibited adenylate cyclase activity, but in this case the inhibition correlated well with the known IC50 values of the drugs for anticalmodulin activity and with their antidiarrhoeal activities.
Longbottom
D
D
Department of Biochemistry, University of Edinburgh, UK.
van Heyningen
S
S
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D014150:Antipsychotic Agents / Q000494:pharmacology*; D002772:Cholera Toxin / Q000494:pharmacology*; D004848:Epithelium / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D008871:Microvilli / Q000378:metabolism; D008970:Molecular Weight; D010640:Phenothiazines / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism; D011817:Rabbits
D000067956:Adenylyl Cyclase Inhibitors; D014150:Antipsychotic Agents; D010640:Phenothiazines; D011506:Proteins; D000246:Adenosine Diphosphate Ribose; D002772:Cholera Toxin; D000262:Adenylyl Cyclases