title: Investigation of soy sauce treated with nitrite in the chromosomal aberration test in vitro and the micronucleus test in vivo.
abstract: Soy sauce pretreated with 2300 ppm nitrite caused no more aberrations than did untreated soy sauce in the chromosomal aberration test in vitro using a Chinese hamster fibroblast cell line with or without S9 mixture. The aberration induction by soy sauce is likely to be caused by the 17% sodium chloride it contains. Soy sauce with or without pretreatment with 2300 ppm nitrite was orally given to ICR mice at a dose of 14 ml/kg body weight once or 6 ml/kg body weight/day for 5 consecutive days. This oral administration did not induce any significant increase in micronuclei in the micronucleus test in vivo.
authors.affiliation: Research and Development Division, Kikkoman Corporation, Chiba, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Nagahara
authors.forename: K
authors.initials: K
authors.lastname: Ohshita
authors.forename: S
authors.initials: S
authors.lastname: Nasuno
mesh_terms: D000818:Animals; D002869:Chromosome Aberrations; D003212:Condiments / Q000009:adverse effects*; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D015162:Micronucleus Tests; D009573:Nitrites / Q000009:adverse effects*; D012965:Sodium Chloride / Q000009:adverse effects; D013025:Glycine max / Q000009:adverse effects*
chemical_list: D009573:Nitrites; D012965:Sodium Chloride