title: Aspartic acid release from cerebral cortical slices of E1 mice with high seizure susceptibility.
abstract: Unlike their parent strain ddY mice, inbred mutant E1 mice are highly susceptible to convulsive seizures upon tossing stimulation. The uptake and release of [3H]aspartate by cerebral neocortical slices were investigated in non-stimulated E1 mice [E1(-)], in stimulated E1 mice [E1(+)] and also in ddY mice using a superfusion system. The uptake and release of aspartate in E1(-) were lower than in E1(+) or ddY. However, the aspartate level in the cerebral cortex of E1(-) was higher compared to that of E1(+) or ddY. Addition of aspartate into the medium resulted to a decreased potassium-stimulated aspartate release in ddY, an increased in E1(-), and no change in E1(+). The results suggest that abnormal state of aspartate release and/or uptake in cerebral cortex of E1 mice may contribute to their increased susceptibility of seizures.
authors.affiliation: Department of Neurochemistry, Okayama University Medical School, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Higashihara
authors.forename: M
authors.initials: M
authors.lastname: Hiramatsu
authors.forename: A
authors.initials: A
authors.lastname: Mori
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D002540:Cerebral Cortex / Q000737:chemistry / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism; D018698:Glutamic Acid; D066298:In Vitro Techniques; D051379:Mice; D008817:Mice, Mutant Strains; D011188:Potassium / Q000494:pharmacology; D012640:Seizures / Q000209:etiology*
chemical_list: D005971:Glutamates; D001224:Aspartic Acid; D018698:Glutamic Acid; D011188:Potassium