Enhancement of antibody production by lysophosphatidylcholine and alkylglycerol.
Inflammation products of normal and cancerous tissues, lysophosphatidylcholine and dodecylglycerol, were tested for their adjuvant effect on the antibody response. Mice treated with these agents and immunized with sheep erythrocytes simultaneously or at 3 days posttreatment developed a greatly enhanced antibody production as demonstrated by the Jerne plaque assay. Mice immunized at 3 days postadministration of agents did not significantly produce enhanced antibody-secreting cells as compared with those of mice simultaneously immunized. Since the mechanism of macrophage activation by lysophospholipids requires contribution of B and T cells, BALB/c-nu/nu mice treated with these agents and subsequently immunized with sheep erythrocytes did not produce antibodies. However, conditioned medium of in vitro-treated BALB/c-nu/nu B cells efficiently transmitted a signal to untreated BALB/c +/+ T cells for enhanced macrophage ingestion activity. This observation suggests that lysophospholipid-activated macrophages and T cells efficiently transmitted antigenic signal to the antibody-producing B cell population. Therefore, we conclude that these lipid metabolites have dual beneficial effects for the host by enhancing phagocytosis and antibody production. Thus, lysophosphatidylcholine and dodecylglycerol have potential practical application as adjuvants that could be administered separately or in combination with antigens.
Ngwenya
B Z
BZ
Department of Microbiology and Immunology, Hahnemann University School of Medicine, Philadelphia, Pennsylvania 19102-1192.
Foster
D M
DM
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000276:immunology; D005989:Glycerides / Q000494:pharmacology*; D006462:Hemolytic Plaque Technique; D007848:Laurates / Q000494:pharmacology*; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D008262:Macrophage Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D050178:Monoglycerides; D012756:Sheep / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D005989:Glycerides; D007848:Laurates; D008244:Lysophosphatidylcholines; D050178:Monoglycerides; C020777:monolaurin