[Study of dipeptidyl peptidase IV as a surface marker of human natural killer cells].
The enriched populations of natural killer (NK) cells have been obtained using two different approaches. The first one was based on the separation in a density gradient of Percoll and further formation of rosettes with sheep erythrocytes. This method allowed to isolate the population containing approximately 70% of CD16(+)-cells. The second approach consisted in separating lymphocytes on a flow cytofluorometer FACS II. Using this method the population with 80% CD16(+)-cells was isolated from PBMC. Studies on morphological, phenotypical and functional characteristics of the first population revealed that NK cells constituted 70% of total number of cells; T-lymphocytes, 8-lymphocytes and monocytes constituted the minor population (10%, 8% and 1% respectively). Activity of DPIV was determined on both cell populations obtained. As it was shown, approximately 27% of the cells isolated using percoll density gradient and 22% of the cells after the separation on a flow cytofluorometer carried the enzyme molecules on the cell surface. The results of the present study apparently indicate that part of NK cells (about 10%) is characterised by the presence of DPIV on the cell surface.
Biuling
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Tonevitskiĭ
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Kiuster
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D002469:Cell Separation; D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000097:blood*; D005434:Flow Cytometry; D006801:Humans; D007694:Killer Cells, Natural / Q000201:enzymology*; D012397:Rosette Formation
D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D018819:Dipeptidyl Peptidase 4