title: Porcine pancreas extract decreases blood-ionized calcium in mice and inhibits osteoclast formation and bone resorption in culture.
abstract: Patients with acute pancreatitis commonly manifest hypocalcemia for reasons which are unknown. We found that porcine pancreas extracts (PX) significantly decreased blood-ionized calcium in Balb/c mice. Partially-purified PX with a molecular mass of approximately 27 kDa decreased blood-ionized calcium in the mice. Partially-purified PX suppressed not only 45Ca release from fetal rat long bones which had been stimulated by parathyroid hormone, interleukin-1 alpha, tumor necrosis factor, transforming growth factor-alpha, 1,25-dihydroxyvitamin D3 and prostaglandin E2, but tartrate-resistant acid phosphatase-positive multinucleated cell formation in the presence of 1,25-dihydroxyvitamin D3 in mouse marrow cultures. The results suggest that there is an as yet-unidentified bone metabolism-regulating substance in porcine pancreas which might be responsible for the hypocalcemia associated with acute pancreatitis.
authors.affiliation: Department of Medicine, University of Texas Health Science Center, San Antonio 78284-7877.
authors.forename: T
authors.initials: T
authors.lastname: Yoneda
authors.forename: Y
authors.initials: Y
authors.lastname: Takaoka
authors.forename: M M
authors.initials: MM
authors.lastname: Alsina
authors.forename: J
authors.initials: J
authors.lastname: Garcia
authors.forename: G R
authors.initials: GR
authors.lastname: Mundy
mesh_terms: D000818:Animals; D001835:Body Weight; D001862:Bone Resorption; D002118:Calcium / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C; D010010:Osteoclasts / Q000502:physiology*; D010179:Pancreas / Q000502:physiology*; D010180:Pancreatectomy; D010281:Parathyroid Hormone / Q000494:pharmacology; D013552:Swine; D014020:Tissue Extracts / Q000494:pharmacology
chemical_list: D010281:Parathyroid Hormone; D014020:Tissue Extracts; D002118:Calcium