title: Postmortem degradation of demineralized bone matrix osteoinductive potential. Effect of time and storage temperature.
abstract: The osteoinductive growth factors present in demineralized bone are degraded by tissue enzymes. Storage of rat limbs at low temperature (4 degrees C) before harvesting of bones preserves the osteoinductive potential of such factors. Storage at room temperature for more than 24 hours causes the recovered bone matrix to be biologically inactive, presumably as the result of biodegradation.
authors.affiliation: University of Southern California Orthopaedic Hospital, Laboratory of Connective Tissue Biochemistry, Los Angeles 90007.
authors.forename: M
authors.initials: M
authors.lastname: Yazdi
authors.forename: S
authors.initials: S
authors.lastname: Bernick
authors.forename: W J
authors.initials: WJ
authors.lastname: Paule
authors.forename: M E
authors.initials: ME
authors.lastname: Nimni
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001857:Bone Matrix / Q000737:chemistry* / Q000637:transplantation; D005612:Freeze Drying; D008297:Male; D010012:Osteogenesis; D051381:Rats; D011919:Rats, Inbred Strains; D013696:Temperature; D013997:Time Factors
chemical_list: D000469:Alkaline Phosphatase