Immune response to Acinetobacter calcoaceticus infection in man.
After growth in an iron-depleted chemically-defined medium Acinetobacter calcoaceticus expressed four high mol. wt outer-membrane proteins (OMPs) which were repressed under iron supplementation or in a complex laboratory medium. Immunoblotting with serum from a septicaemic patient infected with A. calcoaceticus revealed antibody binding to these iron-repressible OMPs, indicating that they were expressed in vivo, and also to the 42- and 18-Kda OMPs. Although the antibody response to the OMPs did not vary significantly during convalescence, the response to the O-polysaccharide component of lipopolysaccharide decreased significantly. However, antibodies in serum from patients with A. calcoaceticus wound infections reacted with the iron repressible OMPs and a 54-Kda antigen suggesting a difference in immune recognition between local and systemic infection.
Smith
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Pharmaceutical Sciences Institute, Aston University, Birmingham.
Alpar
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D000150:Acinetobacter / Q000276:immunology* / Q000378:metabolism; D000151:Acinetobacter Infections / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000097:blood*; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis / Q000276:immunology*; D003470:Culture Media; D006801:Humans; D007501:Iron / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000276:immunology; D018805:Sepsis / Q000276:immunology
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media; D008070:Lipopolysaccharides; D007501:Iron