title: Decreasing gradients of gene conversion on both sides of the initiation site for meiotic recombination at the ARG4 locus in yeast.
abstract: We have constructed eight restriction site polymorphisms in the DED81-ARG4 region and examined their behavior during meiotic recombination. Tetrad analysis reveals decreasing gradients of gene conversion on both sides of the initiation site for meiotic recombination at the ARG4 locus, extending on one side into the ARG4 gene, and on the other side into the adjacent DED81 gene. Gene conversion events can extend in both directions from the initiation site as the result of a single meiotic event. There is a second gradient of gene conversion in DED81, with high levels near the 5' end of the gene and low levels near the middle of the gene. The peaks of gene conversion activity for the DED81 and ARG4 gradients map to regions where double-strand breaks are found during meiosis. The implications of these results for models of meiotic gene conversion are discussed.
authors.affiliation: Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
authors.forename: N P
authors.initials: NP
authors.lastname: Schultes
authors.forename: J W
authors.initials: JW
authors.lastname: Szostak
mesh_terms: D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D003433:Crosses, Genetic; D005785:Gene Conversion; D005800:Genes, Fungal; D005819:Genetic Markers; D008540:Meiosis; D012150:Polymorphism, Restriction Fragment Length; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*
chemical_list: D005819:Genetic Markers