title: Permethylation and tandem mass spectrometry of oligosaccharides having free hexosamine: analysis of the glycoinositol phospholipid anchor glycan from the scrapie prion protein.
abstract: Permethylation of the glycan isolated from the glycoinositol phospholipid (GPI) anchor of the scrapie prion protein (PrPSc) trimethylates a free hexosamine to form a quarternary ammonium salt, substantially increasing the sensitivity for analysis by mass spectrometry. This derivatization induces specific fragmentation reactions in collision-induced dissociation spectra obtained on a four-sector tandem mass spectrometer, identifying the branching pattern of the PrPSc GPI glycan.
authors.affiliation: Department of Pharmaceutical Chemistry, University of California, San Francisco 94143.
authors.forename: M A
authors.initials: MA
authors.lastname: Baldwin
authors.forename: N
authors.initials: N
authors.lastname: Stahl
authors.forename: L G
authors.initials: LG
authors.lastname: Reinders
authors.forename: B W
authors.initials: BW
authors.lastname: Gibson
authors.forename: S B
authors.initials: SB
authors.lastname: Prusiner
authors.forename: A L
authors.initials: AL
authors.lastname: Burlingame
mesh_terms: D006017:Glycolipids / Q000032:analysis*; D017261:Glycosylphosphatidylinositols; D006595:Hexosamines / Q000737:chemistry*; D008745:Methylation; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry*; D010716:Phosphatidylinositols / Q000032:analysis*; D018620:PrPSc Proteins; D011328:Prions / Q000032:analysis*; D013057:Spectrum Analysis; D014764:Viral Proteins / Q000032:analysis* / Q000737:chemistry
chemical_list: D006017:Glycolipids; D017261:Glycosylphosphatidylinositols; D006595:Hexosamines; D009844:Oligosaccharides; D010716:Phosphatidylinositols; D018620:PrPSc Proteins; D011328:Prions; D014764:Viral Proteins