title: Evidence for leucine zipper motif in lactose repressor protein.
abstract: Amino acid sequence homology between the carboxyl-terminal segment of the lac repressor and eukaryotic proteins containing the leucine zipper motif with associated basic DNA binding region (bZIP) has been identified. Based on the sequence comparisons, site-specific mutations have been generated at two sites predicted to participate in oligomer formation based on the three-leucine heptad repeat at positions 342, 349, and 356. Leu342----Ala, Leu349----Ala, and Leu349----Pro have been isolated and their oligomeric state and ligand binding properties evaluated. These mutant proteins do not form tetramers but exist as stable dimers with inducer binding comparable with the wild-type protein. Apparent operator affinities for lac repressor proteins with mutations in the proposed bZIP domain were significantly lower than the corresponding wild-type values. For these dimeric mutant proteins, the monomer-dimer equilibrium is linked to the apparent operator binding constant. The values for the monomer-monomer binding constant and for the intrinsic operator binding constant for the dimer cannot be resolved from measurements of the observed Kd for operator DNA. Further studies on these proteins are in progress.
authors.affiliation: Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77251.
authors.forename: A E
authors.initials: AE
authors.lastname: Chakerian
authors.forename: V M
authors.initials: VM
authors.lastname: Tesmer
authors.forename: S P
authors.initials: SP
authors.lastname: Manly
authors.forename: J K
authors.initials: JK
authors.lastname: Brackett
authors.forename: M J
authors.initials: MJ
authors.lastname: Lynch
authors.forename: J T
authors.initials: JT
authors.lastname: Hoh
authors.forename: K S
authors.initials: KS
authors.lastname: Matthews
mesh_terms: D000595:Amino Acid Sequence; D004252:DNA Mutational Analysis; D066298:In Vitro Techniques; D016350:Leucine Zippers; D008969:Molecular Sequence Data; D009875:Operator Regions, Genetic; D012097:Repressor Proteins / Q000737:chemistry* / Q000235:genetics; D013329:Structure-Activity Relationship
chemical_list: D012097:Repressor Proteins