title: Evaluation of API Coryne in comparison with conventional methods for identifying coryneform bacteria.
abstract: A study was performed to evaluate a new manual miniaturized system, API Coryne (API-bioMérieux, Inc., La Balme les Grottes, France), in which conventional biochemical methods were used to identify 240 isolates of coryneform and related bacteria. A total of 40% of the isolates were excluded from the study because they could not be identified by conventional methods. Identifications of the 240 isolates obtained with API Coryne showed a 97.6% concordance with conventional methods (79% after 24 h of incubation, 21% after 48 h of incubation): 158 (65.8%) isolates were identified with no further testing, and extra testing was required for 76 (31.8%) isolates. In three (1.2%) cases, the organisms did not correspond to any key in the code book and could not be identified by the computer service of the manufacturer. Only three (1.2%) strains were misidentified. The system was shown to be reliable and rapid when compared with standard identification methods.
authors.affiliation: Laboratoire de Bactériologie, Faculté de Pharmacie, Lyon, France.
authors.forename: J
authors.initials: J
authors.lastname: Freney
authors.forename: M T
authors.initials: MT
authors.lastname: Duperron
authors.forename: C
authors.initials: C
authors.lastname: Courtier
authors.forename: W
authors.initials: W
authors.lastname: Hansen
authors.forename: F
authors.initials: F
authors.lastname: Allard
authors.forename: J M
authors.initials: JM
authors.lastname: Boeufgras
authors.forename: D
authors.initials: D
authors.lastname: Monget
authors.forename: J
authors.initials: J
authors.lastname: Fleurette
mesh_terms: D001431:Bacteriological Techniques; D003352:Corynebacterium / Q000145:classification*; D005069:Evaluation Studies as Topic; D006801:Humans