Carassin: a tachykinin that is structurally related to neuropeptide-gamma from the brain of the goldfish.
A 21-amino-acid residue tachykinin-related peptide, carassin, was isolated in pure form from an extract of the brain of the goldfish, Carrassius auratus, by reversed-phase HPLC. The primary structure of the peptide was established as the following: Ser-Pro-Ala-Asn-Ala-Gln-Ile-Thr-Arg-Lys-Arg-His-Lys-Ile-Asn- Ser-Phe-Val-Gly-Leu-Met.NH2. This amino acid sequence is the same length as and shows structural similarity (57% homology) to the mammalian tachykinin, neuropeptide-gamma, which is a product of the posttranslational processing of gamma-preprotachykinin. The mammalian tachykinins, substance P and neurokinin B, were not detected in the extract by using specific antisera directed against the NH2-termini of the peptides, but an antiserum directed against the COOH-terminal region of substance P did detect a low concentration of immunoreactive material.
Conlon
J M
JM
Department of Biomedical Sciences, Creighton University Medical School, Omaha, Nebraska 68178.
O'Harte
F
F
Peter
R E
RE
Kah
O
O
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001921:Brain / Q000378:metabolism*; D006054:Goldfish / Q000378:metabolism*; D013058:Mass Spectrometry; D009479:Neuropeptides / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry*; D011863:Radioimmunoassay; D015320:Tachykinins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*
D000596:Amino Acids; D009479:Neuropeptides; D010446:Peptide Fragments; D015320:Tachykinins; C059905:tachykinin neuropeptide gamma; C067794:carassin