[Immunofluorescent studies on cytoplasmic microtubule complex (CMTC) of Vero cells treated with 5,6-diphenylpyridazin-3-one derivatives].
Effects of six derivatives of 5,6-diphenyl-pyridazin-3-one (DPP) on CMTC of Vero cells were studied by means of the tubulin indirect immunofluorescence technique. Results showed that the effects were different with different derivatives and dose-dependent. Although PD033 had been know to be potent in inhibiting microtubule assembly in vitro, it showed little effect on disturbing CMTC in vivo. Besides having the effects mentioned above, PD032 also induced the formation of free microtubules similar to those induced by taxol.
Liu
Z L
ZL
Institute of Materia Medica, Beijing.
D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D008870:Microtubules / Q000187:drug effects*; D017239:Paclitaxel; D011724:Pyridazines / Q000494:pharmacology*; D011817:Rabbits; D013329:Structure-Activity Relationship; D014709:Vero Cells / Q000187:drug effects
D000470:Alkaloids; D000970:Antineoplastic Agents; D000972:Antineoplastic Agents, Phytogenic; D011724:Pyridazines; C068053:5,6-diphenylpyridazin-3-one; D017239:Paclitaxel