An in vitro clonogenic assay to assess radiation damage in rat CNS glial progenitor cells.
Normal glial progenitor cells can be isolated from the rat central nervous system (CNS) and cultured in vitro on a monolayer of type-1 astrocytes. These monolayers are able to support and stimulate explanted glial progenitor cells to proliferate. Employing these in vitro interactions of specific glial cell types, an in vivo-in vitro clonogenic assay has been developed. This method offers the possibility to study the intrinsic radiosensitivity, repair and regeneration of glial progenitor cells after in vitro or in vivo irradiation.
van der Maazen
R W
RW
Institute of Radiotherapy, University of Nijmegen, The Netherlands.
Verhagen
I
I
van der Kogel
A J
AJ
D000818:Animals; D002470:Cell Survival / Q000528:radiation effects; D002490:Central Nervous System / Q000166:cytology* / Q000528:radiation effects; D003114:Colony-Forming Units Assay / Q000379:methods*; D004307:Dose-Response Relationship, Radiation; D066298:In Vitro Techniques; D009457:Neuroglia / Q000528:radiation effects*; D051381:Rats; D011919:Rats, Inbred Strains; D013234:Stem Cells / Q000528:radiation effects*