title: Inactivation of bacterial glutamine synthetase by ADP-ribosylation.
abstract: Glutamine synthetase from Escherichia coli was inactivated by chemical modification with arginine-specific reagents (Colanduoni, J. A., and Villafranca, J. J. (1985) Biochem. Biophys. Res. Commun. 126, 412-418). E. coli glutamine synthetase was also a substrate for an erythrocyte NAD:arginine ADP-ribosyltransferase. Transfer of one ADP-ribosyl group/subunit of glutamine synthetase caused loss of both biosynthetic and gamma-glutamyltransferase activity. The ADP-ribose moiety was enzymatically removed by an erythrocyte ADP-ribosylarginine hydrolase, resulting in return of function. The site of ADP-ribosylation was arginine 172, determined by isolation of the ADP-ribosylated tryptic peptide. Arginine 172 lies in a central loop that extends into the core formed by the 12 subunits of the native enzyme. The central loop is important in anchoring subunits together to yield the spatial orientation required for catalytic activity. ADP-ribosylation may thus inactivate glutamine synthetase by disrupting the normal subunit alignment. Enzyme-catalyzed ADP-ribosylation may provide a simple, specific technique to probe the role of arginine residues in the structure and function of proteins.
authors.affiliation: Laboratory of Cellular Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: J
authors.initials: J
authors.lastname: Moss
authors.forename: S J
authors.initials: SJ
authors.lastname: Stanley
authors.forename: R L
authors.initials: RL
authors.lastname: Levine
mesh_terms: D036002:ADP Ribose Transferases / Q000097:blood*; D000596:Amino Acids / Q000032:analysis; D004912:Erythrocytes / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology*; D005974:Glutamate-Ammonia Ligase / Q000037:antagonists & inhibitors*; D006801:Humans; D007700:Kinetics; D010446:Peptide Fragments / Q000302:isolation & purification
chemical_list: D000596:Amino Acids; D010446:Peptide Fragments; D036002:ADP Ribose Transferases; D005974:Glutamate-Ammonia Ligase