title: Preliminary studies on the indium slide immunoassay for estimation of human chorionic gonadotropin and antihuman chorionic gonadotropin antibody.
abstract: Human chorionic gonadotropin (hCG) is synthesized and secreted as early as 170 hr after fertilization and has been used as an index for pregnancy. Neutralization of hCG with a beta-subunit hCG vaccine(s) has been proposed as a contraceptive technique. To monitor the duration of effectiveness of the vaccine, it will be necessary to monitor the anti-hCG antibodies, especially those responsible for inhibiting the hCG bioactivity. We report a simple, rapid technique using an indium slide immunoassay for the qualitative estimation of hCG and to monitor a bioeffective anti-hCG antibody. The sensitivity of the indium slide assay to measure hCG ranged from 1 microgram/ml to 1 ng/ml, depending on the format of the assay. The indium slide assay also detected anti-hCG antibodies generated against a specific determinant on hCG recognized by a neutralizing monoclonal antibody (P3W80) in women immunized with a contraceptive vaccine.
authors.affiliation: National Institute of Immunology, New Delhi, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Gupta
authors.forename: G P
authors.initials: GP
authors.lastname: Talwar
authors.forename: N R
authors.initials: NR
authors.lastname: Rose
authors.forename: C L
authors.initials: CL
authors.lastname: Burek
mesh_terms: D000906:Antibodies / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D006063:Chorionic Gonadotropin / Q000097:blood / Q000276:immunology*; D003269:Contraception, Immunologic / Q000379:methods; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007204:Indium; D011247:Pregnancy; D014612:Vaccines / Q000276:immunology
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D006063:Chorionic Gonadotropin; D014612:Vaccines; D007204:Indium