Amplification and expression of the c-erb B-2/neu proto-oncogene in human bladder cancer.
The c-erb B-2/neu gene encodes a cell-surface glycoprotein with extensive homology to, but distinct from, the epidermal growth-factor receptor. In this study, we compared the c-erb B-2/neu gene amplification and expression of tissue specimens obtained from the bladders of normal controls and patients with high-grade transitional cell bladder carcinoma. Southern blot analysis of DNAs from 24 patients and 5 controls showed 2 cases of c-erb B-2/neu gene amplification in patients and none in controls. Western blot analysis demonstrated that c-erb B-2/neu was expressed in 67.6% (23/34) of patient specimens but in none of the controls (0/5). This finding agreed with the result of immunohistochemical staining, which showed that tissue from 74.3% (26/35) of the patients and none of the controls (0/7) showed positive immunofluorescence staining. This is the first report suggesting that c-erb B-2/neu gene amplification may be associated with human bladder carcinogenesis.
Zhau
H E
HE
Department of Urology, University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030.
Zhang
X
X
von Eschenbach
A C
AC
Scorsone
K
K
Babaian
R J
RJ
Ro
J Y
JY
Hung
M C
MC
D015139:Blotting, Southern; D015153:Blotting, Western; D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000276:immunology; D004273:DNA, Neoplasm / Q000235:genetics; D005455:Fluorescent Antibody Technique; D005784:Gene Amplification; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics / Q000276:immunology; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D018719:Erb-b2 Receptor Tyrosine Kinases; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000276:immunology
D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases; C000715470:MAS1 protein, human