title: Growth inhibition of human keratinocytes by antisense c-myc oligomer is not coupled to induction of differentiation.
abstract: To study the relationship between cell growth and differentiation in human keratinocytes, we examined the effect of the antisense oligomer of c-myc mRNA. This oligomer is stable in culture medium. A 24 h incubation of cells with 5 microM antisense c-myc oligomer resulted in a 48.2% decrease in c-myc protein and inhibited cell growth by 80.7% compared to the sense c-myc oligomer. In contrast, antisense c-myc oligomer had no effect on differentiation when the population of involucrin-positive cells and cornified envelope formation were used as differentiation markers. These results show that antisense c-myc oligomer inhibits cell growth but does not induce differentiation in normal human keratinocytes. Therefore, cell growth and differentiation are not necessarily coupled in these cells.
authors.affiliation: Department of Dermatology, Osaka University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Hashiro
authors.forename: K
authors.initials: K
authors.lastname: Matsumoto
authors.forename: H
authors.initials: H
authors.lastname: Okumura
authors.forename: K
authors.initials: K
authors.lastname: Hashimoto
authors.forename: K
authors.initials: K
authors.lastname: Yoshikawa
mesh_terms: D001483:Base Sequence; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D002455:Cell Division; D002478:Cells, Cultured; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects; D008969:Molecular Sequence Data; D016372:RNA, Antisense / Q000235:genetics*; D012333:RNA, Messenger / Q000737:chemistry
chemical_list: D016372:RNA, Antisense; D012333:RNA, Messenger; D001973:Bromodeoxyuridine