The effect of some anti-inflammatory agents on elastase release from neutrophils in-vitro.
In view of the potential role of released polymorphonuclear leucocyte elastase in causing tissue damage, the effect of commonly used anti-inflammatory drugs on elastase release from neutrophils has been studied in-vitro. Elastase release from neutrophils exposed to the synthetic bacterial cell wall peptide N-formyl-L-methionyl-L-leucyl-L-phenylalanine (10(-6) M) was quantitated using a radiometric immunoassay and a functional assay of elastase. Prednisolone and non-steroidal anti-inflammatory drugs inhibited elastase release at concentrations from 0.1 mM-0.1 nM. No inhibition by sulphosalicylic acid, D-penicillamine or chloroquine sulphate was observed. The clinical relevance of these findings is discussed.
Adeyemi
E O
EO
Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Chadwick
V S
VS
Hodgson
H J
HJ
D000328:Adult; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000378:metabolism*; D011239:Prednisolone / Q000494:pharmacology; D011863:Radioimmunoassay
D000893:Anti-Inflammatory Agents; D009240:N-Formylmethionine Leucyl-Phenylalanine; D011239:Prednisolone; D010196:Pancreatic Elastase; D007213:Indomethacin