DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.
Molecular genetic maps are commonly constructed by analyzing the segregation of restriction fragment length polymorphisms (RFLPs) among the progeny of a sexual cross. Here we describe a new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence. These polymorphisms, simply detected as DNA segments which amplify from one parent but not the other, are inherited in a Mendelian fashion and can be used to construct genetic maps in a variety of species. We suggest that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
Williams
J G
JG
Central Research and Development Department, E.I. du Pont de Nemours & Co., Inc, Wilmington, DE 19880.
Kubelik
A R
AR
Livak
K J
KJ
Rafalski
J A
JA
Tingey
S V
SV
D001482:Base Composition; D001483:Base Sequence; D003434:Crossing Over, Genetic; D004247:DNA / Q000096:biosynthesis / Q000737:chemistry*; D005819:Genetic Markers; D006801:Humans; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000235:genetics; D009710:Nucleotide Mapping; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013025:Glycine max / Q000235:genetics; D003313:Zea mays / Q000235:genetics
D005819:Genetic Markers; D004247:DNA