Ca2(+)-mobilizing receptors of gastrulating chick embryo.
1. Gastrulating chick embryo cells (stages 3-5 by HH) possess Ca2(+)-mobilizing receptors for ACh and ATP; insulin and noradrenaline have a weaker effect on [Ca2+]i mobilization. 2. The ED50 value for ACh is 4 (+/- 0.5) X 10(-6) M and for ATP 20 (+/- 5) X 10(-6) M. 3. Addition of ACh and ATP to dissociated chick embryo cells causes rapid accumulation of IP3. 4. The stimulatory effects of ACh and ATP on [Ca2+]i mobilization and IP3 rapid formation are both additive.
Laasberg
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Laboratory of Molecular Genetics, Institute of Chemical Physics and Biophysics, Estonia, U.S.S.R.
D000109:Acetylcholine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002642:Chick Embryo; D005775:Gastrula / Q000502:physiology*; D066298:In Vitro Techniques; D007295:Inositol Phosphates / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000502:physiology*
D007295:Inositol Phosphates; D007328:Insulin; D010716:Phosphatidylinositols; D011956:Receptors, Cell Surface; D000255:Adenosine Triphosphate; D000109:Acetylcholine; D002118:Calcium; D009638:Norepinephrine