title: The lateral spread of ictal discharges in neocortical brain slices.
abstract: The in vitro brain slice technique was used to examine the lateral propagation of spontaneous electrographic ictal episodes across adjacent areas of guinea pig neocortex. Epileptiform activity was induced by perfusing slices with Mg-free artificial CSF. Simultaneous field potential recordings of ictal episodes were obtained from 4 micropipettes placed 1-3 mm apart across coronal slices in middle-cortical layers. Two types of lateral spread were characterized. Ictal episodes often developed focally and then spread as a slowly moving wavefront traveling at less than 0.3 mm/sec into adjacent, uninvolved cortex. By contrast, other episodes began nearly synchronously at all cortical sites. The individual afterdischarges that composed each ictal episode propagated rapidly across the cortex at greater than 30 mm/sec and were triggered by multiple pacemakers. Ictal episodes always terminated abruptly across the entire slice. The NMDA-receptor antagonist, 2-amino-phosphono-valerate, applied focally between recording sites, blocked rapid propagation across treated areas and resulted in the emergence of spatially separate, independent pacemakers. Pacemaker failure is the proposed mechanism for simultaneous and generalized termination of ictal episodes in this in vitro model of epileptogenesis.
authors.affiliation: Department of Neurology and Neurological Sciences, Stanford University School of Medicine, CA 94305.
authors.forename: B Y
authors.initials: BY
authors.lastname: Wong
authors.forename: D A
authors.initials: DA
authors.lastname: Prince
mesh_terms: D015763:2-Amino-5-phosphonovalerate / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000503:physiopathology*; D004558:Electric Stimulation / Q000379:methods; D004594:Electrophysiology; D004827:Epilepsy / Q000503:physiopathology*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D009431:Neural Conduction / Q000187:drug effects; D013997:Time Factors
chemical_list: D015763:2-Amino-5-phosphonovalerate