title: Characterisation of neurotensin-immunoreactivity in porcine ileum using region-specific radioimmunoassays and chromatographic fractionation: isolation and primary structure of porcine neurotensin.
abstract: 1. Neurotensin-immunoreactivity has been characterised in porcine ileum using region-specific radioimmunoassay coupled to chromatographic fractionation. 2. Two immunoreactive peptides were identified. 3. Peptide 1 was immunochemically, chromatographically and structurally identical to bovine neurotensin. 4. Peptide 2 exhibited novel immunochemical and chromatographic characteristics and represents a new neurotensin-related peptide.
authors.affiliation: Department of Medicine, Queen's University of Belfast, Northern Ireland.
authors.forename: C
authors.initials: C
authors.lastname: Shaw
authors.forename: L
authors.initials: L
authors.lastname: Thim
authors.forename: C F
authors.initials: CF
authors.lastname: Johnston
authors.forename: K D
authors.initials: KD
authors.lastname: Buchanan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007082:Ileum / Q000737:chemistry* / Q000276:immunology; D008969:Molecular Sequence Data; D009496:Neurotensin / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D011863:Radioimmunoassay / Q000379:methods; D013552:Swine
chemical_list: D009496:Neurotensin