title: [Densitometric analysis of aminopeptidase M activity in small intestine mucosa in piglets experimentally infected with Isospora suis].
abstract: In gnotobiotical and conventional piglets infected a day post partum (DPP) with oocysts of the coccidium Isospora suis, densitometrical analysis of the activity of aminopeptidase M (EC.3.4.11.2; APM) was performed in the area of microvillous zone of the small intestine. Piglets were infected with different infection doses of oocysts (100,000 oocysts) in gnotobiotical piglets and 200,000 oocysts in conventional piglets). In infected gnotobiotical piglets, the APM activity was studied in the period from the 3rd to 11th day after infection (DAI) and in infected conventional piglets in the period of to the 2nd to 10th day after infection (DAI). Control piglets, in the group of the gnotobiotical animals at the age of 2 to 5 days in the group of the conventional animals at the age of 4 to 7 days, had different APM activity in the microvillous zone of the intestinal mucosa. It was stated that the microvillous zone of the intestinal mucosa gained higher values in control conventional piglets (+7.01 mean values of density). In infected gnotobiotical piglets the density fall of the reaction product of APM was demonstrated already on the third day with further marked reduction of APM density on the 4th day after infection in the whole small intestine with predominance of the persisting APM activity in ileum. Even despite the slight increase in the density of the reaction product of APM in the period from the 5th to 7th DAI (the highest increase in APM density on the 6th DAI), a further decrease of the activity was recorded again on the 8th and namely the 9th DAI in the whole small intestine (the lowest value of density was found in the rear jejunum), the ileum mucosa being affected, too. A slightly higher density of the reaction product of APM was found in the duodenum. On the 10th DAI the APM density started to change and on the 11th DAI in the duodenum and in the middle jejunum it even reached higher values in comparison with the control data. Some differences were proved in the infected conventional piglets in comparison with the development of the APM activity in the small intestine mucosa in the infected gnotobiotical piglets. On the 3rd and 4th DAI APM defect occurred in the whole small intestine, with APM density prevailing in the ileum mucosa (like in the group of infected gnotobiotical piglets). The second period of decrease in APM activity lasted for almost four days (6th to 9th DAI).(ABSTRACT TRUNCATED AT 400 WORDS)
authors.affiliation: Parazitologický ústav CSAV, Ceské Budĕjovice.
authors.forename: M
authors.initials: M
authors.lastname: Kudweis
authors.forename: I
authors.initials: I
authors.lastname: Julis
authors.forename: Z
authors.initials: Z
authors.lastname: Lojda
authors.forename: J
authors.initials: J
authors.lastname: Vítovec
authors.forename: B
authors.initials: B
authors.lastname: Koudela
mesh_terms: D000626:Aminopeptidases / Q000378:metabolism*; D000818:Animals; D018826:CD13 Antigens; D003048:Coccidiosis / Q000201:enzymology / Q000662:veterinary*; D003720:Densitometry; D005856:Germ-Free Life; D006651:Histocytochemistry; D007413:Intestinal Mucosa / Q000201:enzymology; D007421:Intestine, Small / Q000201:enzymology*; D013552:Swine; D013553:Swine Diseases / Q000201:enzymology*
chemical_list: D000626:Aminopeptidases; D018826:CD13 Antigens