Identification of meningeal cell released neurite promoting activities for embryonic hippocampal neurons.
Primary cultures of meningeal cells from embryonic rat cerebra secrete neurite growth-inducing components into serum-free culture medium. This conditioned medium (CM) was analyzed by FPLC and immunochemical and enzymatic treatments and tested for neurite promoting activity (NPA) in a quantitative bioassay using hippocampal neurons from embryonic rat. By immunoprecipitation or specific adsorption we identified laminin (LN)-proteoglycan complexes and fibronectin (FN), respectively, as the major neurite promoting components within meningeal cell CM. The LN-proteoglycan complexes and their NPA were sensitive to chondroitinase (chondroitin ABC lyase, EC 4.2.2.4) and to a smaller extent to heparitinase (heparitin sulfate lyase, EC 4.2.2.8). Minor fractions of the total NPA in CM correlated with free LN and a putative but not yet characterized FN-proteoglycan complex.
Matthiessen
H P
HP
Molecular Neurobiology Laboratory, Department of Neurology, University of Düsseldorf, F.R.G.
Schmalenbach
C
C
Müller
H W
HW
D000818:Animals; D001369:Axons / Q000502:physiology*; D002478:Cells, Cultured; D002810:Chondroitinases and Chondroitin Lyases / Q000494:pharmacology; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D005353:Fibronectins / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D007797:Laminin / Q000378:metabolism; D008578:Meninges / Q000196:embryology / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism / Q000502:physiology; D051381:Rats / Q000196:embryology; D011919:Rats, Inbred Strains
D003470:Culture Media; D005353:Fibronectins; D006025:Glycosaminoglycans; D007797:Laminin; D002810:Chondroitinases and Chondroitin Lyases