title: Expression of class II molecules on intestinal epithelial cells in humans. Differences between normal and inflammatory bowel disease.
abstract: Expression of class II antigens on human intestinal epithelial cells was assessed using a sensitive avidinbiotin-peroxidase technique. HLA-DR was present predominantly in the normal small bowel with diminished but evident expression in the colon. HLA-DP staining was less prominent, and HLA-DQ was absent. In inflammatory bowel disease the expression of both HLA-DR and HLA-DP was increased, but that for HLA-DQ remained absent, suggesting an inherent defect in the ability of intestinal epithelial cells to express HLA-DQ. In related experiments, an interferon gamma-treated malignant epithelial cell line T84 also failed to stain for HLA-DQ and HLA-DP despite the presence of HLA-DR. Isolated RNAs for all three subclasses of HLA-D were detectable by slot-blot analysis, suggesting that the lack of HLA-DQ expression relates to posttranscriptional defects in intestinal epithelium. These and other differences with conventional class II antigen-positive accessory cells (macrophages/B cells) may help to explain the unique properties of intestinal epithelial cells as antigen-presenting cells.
authors.affiliation: Division of Clinical Immunology, Mount Sinai Medical Center, New York, New York.
authors.forename: L
authors.initials: L
authors.lastname: Mayer
authors.forename: D
authors.initials: D
authors.lastname: Eisenhardt
authors.forename: P
authors.initials: P
authors.lastname: Salomon
authors.forename: W
authors.initials: W
authors.lastname: Bauer
authors.forename: R
authors.initials: R
authors.lastname: Plous
authors.forename: L
authors.initials: L
authors.lastname: Piccinini
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology; D003110:Colonic Neoplasms / Q000276:immunology; D005786:Gene Expression Regulation; D006681:HLA-D Antigens / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000276:immunology*; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: D006681:HLA-D Antigens