Reconstruction of the skin in three-dimensional collagen gel matrix culture.
The skin comprises three layers: epidermis, dermis, and hypodermis. We report here on a skin, reconstructed in vitro, that is composed of all three layers. The top-most layer, epidermis, was exposed to air by a new method. The exposure induced an extensive proliferation, and differentiation, i.e. keratinization was eventually observed in the cultured epidermal cells. Skin thus cultured will be a useful graft of transplantation and provide an ideal model system in which to study diseases of the skin.
Sugihara
H
H
Department of Pathology, Saga Medical School, Japan.
Toda
S
S
Miyabara
S
S
Kusaba
Y
Y
Minami
Y
Y
D000388:Air; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen; D000078404:Epidermal Cells; D004817:Epidermis / Q000502:physiology; D005782:Gels; D008722:Methods; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000166:cytology* / Q000648:ultrastructure; D012879:Skin Physiological Phenomena
D005782:Gels; D003094:Collagen