title: Complementation between Sindbis viral RNAs produces infectious particles with a bipartite genome.
abstract: Sindbis virus, the type member of the alpha-viruses, is an enveloped virus containing a nonsegmented positive-strand RNA genome. We show that the nonstructural and the structural genes can function to produce infectious virus particles when they are expressed on two different RNA segments. The nonstructural genes are translated from an RNA in which the structural genes have been replaced by the chloramphenicol acetyltransferase gene [Xiong, C., Levis, R., Shen, P., Schlesinger, S., Rice, C. M. & Huang, H. V. (1989) Science 243, 1188-1191]. The structural genes are encoded in a defective-interfering RNA but are translated from a subgenomic RNA. Both segments contain the cis-acting sequences required for replication and packaging and are copackaged. This type of genome provides a model for an ancestral intermediate between alphaviruses and the multipartite positive-strand RNA viruses of plants. These different viruses show sequence similarities in their replicative proteins and are thought to have evolved from a common ancestor.
authors.affiliation: Department of Molecular Microbiology, Washington University Medical School, St. Louis, MO 63110-1093.
authors.forename: U
authors.initials: U
authors.lastname: Geigenmüller-Gnirke
authors.forename: B
authors.initials: B
authors.lastname: Weiss
authors.forename: R
authors.initials: R
authors.lastname: Wright
authors.forename: S
authors.initials: S
authors.lastname: Schlesinger
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D005455:Fluorescent Antibody Technique; D005814:Genes, Viral; D005816:Genetic Complementation Test; D005818:Genetic Engineering; D009024:Morphogenesis; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D012845:Sindbis Virus / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection; D015678:Viral Structural Proteins / Q000235:genetics; D014779:Virus Replication
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D015678:Viral Structural Proteins