Binding of long-chain fatty acids to serum albumin in healthy humans. Relationship to obesity.
Equilibria of the binding of palmitate to serum albumin in adults are studied by dialysis-exchange-rate determinations. The results are used for a description of binding equilibria of fatty acids in general, as follows. 1. The reserve albumin concentration, p, for binding of palmitate is used as an approximate measure of p*, the reserve albumin concentration for binding of mixed fatty acids present in serum. 2. The total availability of fatty acids is defined as C*/p*, where C* is the total concentration of non-esterified fatty acid. 3. The fatty-acid-binding property of albumin is described by L* = p*/P = alpha C*/P, where P is the albumin concentration. The numerical value of alpha is -0.05. The above parameters are measured in sera from four healthy volunteers, in whom large variations of serum fatty acid concentration occurred. A group of 64 healthy students showed considerable variation of L* from one individual to another. It is found that L* decrease significantly with increasing body mass index (body mass divided by the square of the body length). In 42 patients with diabetes type I, L* was independent of body mass index. These findings are consistent with a previously formulated hypothesis of mechanism of obesity.
Brodersen
R
R
Institute of Medical Biochemistry, University of Aarhus, Denmark.
Vorum
H
H
Krukow
N
N
Pedersen
A O
AO
D000328:Adult; D001665:Binding Sites; D001835:Body Weight; D002940:Circadian Rhythm; D005227:Fatty Acids / Q000097:blood*; D006801:Humans; D008297:Male; D010168:Palmitates / Q000378:metabolism; D012709:Serum Albumin / Q000378:metabolism*
D005227:Fatty Acids; D010168:Palmitates; D012709:Serum Albumin