The putative role of fibrin fragments in the biosynthesis of fibrinogen by hepatoma cells.
The current study reinvestigated whether pure plasminolytic fragments D and E from fibrin directly increase fibrinogen synthesized by the hepatocyte. Fibrinogen protein levels and fibrinogen mRNA levels in an interleukin-6 (IL-6) responsive rat hepatoma cell line (FAZA) were measured quantitatively by [35S]-methionine pulse-chase experiments and Northern hybridizations, respectively. The results demonstrate that neither fragments D nor E, alone or in combination, in the presence or absence of dexamethasone, had any influence on the production of fibrinogen.
Wang
Y
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Department of Cell Biology, University of Alabama, Birmingham 35294.
Fuller
G M
GM
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D003907:Dexamethasone / Q000494:pharmacology; D005338:Fibrin Fibrinogen Degradation Products / Q000502:physiology*; D005340:Fibrinogen / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D066298:In Vitro Techniques; D015850:Interleukin-6 / Q000502:physiology*; D008099:Liver / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D014407:Tumor Cells, Cultured
D005338:Fibrin Fibrinogen Degradation Products; D015850:Interleukin-6; D012333:RNA, Messenger; D003907:Dexamethasone; D005340:Fibrinogen