A noncholinergic action of acetylcholinesterase (AChE) in the brain: from neuronal secretion to the generation of movement.
1. In various brain regions, there is a puzzling disparity between large amounts of acetylcholinesterase and low levels of acetylcholine. One such area is the substantia nigra. Furthermore, within the substantia nigra, a soluble form of acetylcholinesterase is released from the dendrites of dopamine-containing nigrostriatal neurons, independent of cholinergic transmission. These two issues have prompted the hypothesis that acetylcholinesterase released in the substantia nigra has an unexpected noncholinergic function. 2. Electrophysiological studies demonstrate that this dendritic release is a function, not of the excitability of the cell from which the acetylcholinesterase is released, but of the inputs to it. In order to explore this phenomenon at the behavioral level, a novel system has been developed for detecting release of acetylcholinesterase "on-line." It can be seen that release of this protein within the substantia nigra can reflect, but is not causal to, movement. 3. Once released, the possible actions of acetylcholinesterase can be studied at both the cellular and the behavioral level. Independent of its catalytic site, acetylcholinesterase has a "modulatory" action on nigrostriatal neurons. The functional consequences of this modulation would be to enhance the sensitivity of the cells to synaptic inputs. 4. Many basic questions remain regarding the release and action of acetylcholinesterase within the substantia nigra and, indeed, within other areas of the brain. Nonetheless, tentative conclusions can be formulated that begin, in a new way, to provide a link between cellular mechanisms and the control of movement.
Greenfield
S A
SA
University Department of Pharmacology, Oxford, U.K.
D000109:Acetylcholine / Q000502:physiology; D000110:Acetylcholinesterase / Q000502:physiology*; D000818:Animals; D002795:Choline O-Acetyltransferase / Q000502:physiology; D004721:Endoplasmic Reticulum / Q000201:enzymology; D008564:Membrane Potentials / Q000187:drug effects; D008565:Membrane Proteins / Q000378:metabolism; D008954:Models, Biological; D009068:Movement / Q000502:physiology; D009474:Neurons / Q000378:metabolism; D051381:Rats; D012636:Secretory Rate / Q000187:drug effects; D015398:Signal Transduction; D013378:Substantia Nigra / Q000201:enzymology*
D008565:Membrane Proteins; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase; D000109:Acetylcholine