Potentiation of antigen-induced mast cell activation by 1-34 bovine parathyroid hormone.
Peptides such as parathyroid hormone (PTH), somatostatin, and gastrin have been reported to stimulate mast cell mediator release. Preincubation of rat serosal mast cells with synthetic 1-34 bovine parathyroid hormone (1-34bPTH) significantly enhanced antigen-induced 5-hydroxytryptamine (5-HT) release. Enhancement of 5-HT release by 1-34bPTH was dose dependent between 5 and 2000 nM. In the absence of antigen, mean net 5-HT release was less than 1% when naive or passively sensitized mast cells were incubated with 1000 nM 1-34bPTH for time intervals up to 90 min. These findings indicate that 1-34bPTH, at relatively low concentration, potentiates antigen-induced 5-HT release from mast cells.
Simpson
K M
KM
Department of Internal Medicine, University of Kansas Medical Center, Kansas City 66103.
Dileepan
K N
KN
Stechschulte
D J
DJ
D000818:Animals; D000941:Antigens; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D007700:Kinetics; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology*; D010281:Parathyroid Hormone / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism; D012710:Serum Albumin, Bovine; D013997:Time Factors
D000941:Antigens; D010281:Parathyroid Hormone; D010446:Peptide Fragments; C025076:parathyroid hormone (1-34), bovine; D012710:Serum Albumin, Bovine; D012701:Serotonin; D000001:Calcimycin