Quantitative approaches for studying gene expression.
The methods currently available for measuring mRNAs and proteins are reviewed, with a special emphasis on their application to physiological questions. The article focuses on the quantitative determination of cellular contents, but also on assessment of rates of synthesis and degradation, and turnover.
Wiesner
R J
RJ
Department of Medicine, University of Chicago, Illinois 60637.
Zak
R
R
D000818:Animals; D015870:Gene Expression; D005821:Genetic Techniques; D007700:Kinetics; D008433:Mathematics; D008954:Models, Biological; D009693:Nucleic Acid Hybridization; D011506:Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism
D011506:Proteins; D012333:RNA, Messenger