title: Extended C-terminal tail of wheat histone H2A interacts with DNA of the "linker" region.
abstract: The preparation of hybrid histone octamers with wheat histone H2A variants replacing chicken H2A in the chicken octamer is described. The fidelity of the reconstituted hybrid octamers was confirmed by dimethyl suberimidate cross-linking. Polyglutamic-acid-mediated assembly of these octamers on long DNA and subsequent micrococcal nuclease (MNase) digestion demonstrated that, whereas chicken octamers protected 167 base-pairs (representing 2 full turns of DNA), hybrid histone octamers containing wheat histone H2A(1) with its 19 amino acid residue C-terminal extension protected an additional 16 base pairs of DNA against nuclease digestion. The protection observed by hybrid histone octamers containing wheat histone H2A(3) with both a 15 residue N-terminal and a 19 residue C-terminal extension was identical with that observed with H2A(1)-containing hybrid histone octamers with only the 19 residue C-terminal extension. These results suggest that the role of the C-terminal extension is to bind to DNA of the "linker" region. The thermal denaturation of chicken and hybrid core particles was identical in 10 mM-Tris.HCl.20 mM-NaCl, 0.1 mM-EDTA, confirming that there was no interaction between the basic C-terminal extension and DNA of the core particle. Denaturation in EDTA, however, showed that hybrid core particles had enhanced stability, suggesting that the known conformational change of core particles at very low ionic strength allows the C-terminal extension to bind to core particle DNA under these conditions. A model accounting for the observed MNase protection is presented.
authors.affiliation: Department of Biochemistry, University of Cape Town, Republic of South Africa.
authors.forename: G G
authors.initials: GG
authors.lastname: Lindsey
authors.forename: S
authors.initials: S
authors.lastname: Orgeig
authors.forename: P
authors.initials: P
authors.lastname: Thompson
authors.forename: N
authors.initials: N
authors.lastname: Davies
authors.forename: D L
authors.initials: DL
authors.lastname: Maeder
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001482:Base Composition; D002645:Chickens; D004247:DNA / Q000378:metabolism*; D014644:Genetic Variation; D006657:Histones / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011489:Protein Denaturation; D014908:Triticum / Q000235:genetics*
chemical_list: D006657:Histones; D004247:DNA