Lipid arrangement in fluid model membranes: analysis by cross-linking of phosphatidylethanolamines.
Binary mixtures of fluid phase phosphatidylethanolamines at pH 10 were treated with the bifunctional cross-linking reagent dimethylsuberimidate. Analysis of the dimeric species formed demonstrated that the phospholipid species in dimyristoylphosphatidylethanolamine/dielaidoylphosphatidylethanolamine mixtures at 52 degrees C and dielaidoylphosphatidylethanolamine/dilauroylphosphatidylethanolamine mixtures at 41 degrees C were randomly arranged. Analysis of the dimeric species formed in dipalmitoylphosphatidylethanolamine/dioleoylphosphatidylethanolamine mixtures at 68 degrees C showed that this mixture was very close to being randomly arranged, with just a slight propensity of like phospholipid species to cluster.
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Division of Biology, Kansas State University, Manhattan 66506.
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D003432:Cross-Linking Reagents; D004120:Dimethyl Suberimidate / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008560:Membrane Fluidity; D008563:Membrane Lipids; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D013696:Temperature
D003432:Cross-Linking Reagents; D008563:Membrane Lipids; D010714:Phosphatidylethanolamines; D004120:Dimethyl Suberimidate