Partial deletion of the Rhizobium phaseoli CFN23 symbiotic plasmid implies a concomitant amplification of plasmid DNA sequences.
Rhizobium leguminosarum biovar phaseoli CFN23 loses its ability to nodulate beans at a high frequency because of a deletion of part of its symbiotic (pSym) plasmid (Soberón-Chávez et al., 1986). We report here that at least 80 kb of pSym are deleted upon loss of the symbiotic phenotype; the deletion removes the nitrogenase structural nifHDK and the common nodABC genes. The size of the deleted pSym is not reduced, since it is accompanied by an amplification of other pSym plasmid sequences. This genetic rearrangement is similar to the deletion and amplification of yeast mitochondrial DNA leading to 'petite' mutations.
Soberón-Chávez
G
G
Centro de Investigación sobre Ingeniería Genética y Biotecnología, UNAM, Cuernavaca, Morelos, Mexico.
Nájera
R
R
Calva
E
E
Fernández
M
M
D015139:Blotting, Southern; D002872:Chromosome Deletion; D004269:DNA, Bacterial / Q000096:biosynthesis*; D007887:Fabaceae / Q000382:microbiology; D005784:Gene Amplification; D005798:Genes, Bacterial; D009586:Nitrogen Fixation / Q000235:genetics; D009591:Nitrogenase / Q000235:genetics; D010641:Phenotype; D010946:Plants, Medicinal; D010957:Plasmids / Q000235:genetics*; D012231:Rhizobium / Q000235:genetics*; D013559:Symbiosis / Q000235:genetics*
D004269:DNA, Bacterial; D009591:Nitrogenase