Transcription is associated with Z-DNA formation in metabolically active permeabilized mammalian cell nuclei.
Mammalian cells have been encapsulated in agarose microbeads, and from these cells metabolically active permeabilized nuclei were prepared. Previously, we showed that biotin-labeled monoclonal antibodies against Z-DNA can be diffused into the nuclei and, over a specific concentration range, they will bind to Z-DNA within the nucleus in a concentration-independent manner. By using radiolabeled streptavidin, we showed that the amount of Z-DNA antibody bound is related to the torsional strain of the DNA in the nucleus. Relaxation of the DNA results in a decrease of Z-DNA formation, whereas increasing torsional strain through inhibiting topoisomerase I results in increased Z-DNA formation. Here we measure the influence of RNA transcription and DNA replication. Transcription is associated with a substantial increase in the binding of anti-Z-DNA antibodies, paralleling the increased level of RNA synthesized as the level of ribonucleoside triphosphate in the medium is increased. DNA replication yields smaller increases in the binding of Z-DNA antibodies. Stopping RNA transcription with inhibitors results in a large loss of Z-DNA antibody binding, whereas only a small decrease is associated with inhibition of DNA replication.
Wittig
B
B
Institut für Molekularbiologie und Biochemie, Freie Universität, Berlin, Federal Republic of Germany.
Dorbic
T
T
Rich
A
A
D000911:Antibodies, Monoclonal; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D004261:DNA Replication; D004273:DNA, Neoplasm / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D010539:Permeability; D014158:Transcription, Genetic
D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm