title: Detection of transmembrane linkages between immunoglobulin or complement receptors and the neutrophil's cortical microfilaments by resonance energy transfer microscopy.
abstract: By exploiting the 1/r4 (where r is the separation distance between fluorochromes) dependence of energy transfer between parallel lamellae, we have observed transmembrane energy transfer between membrane receptors and cortical microfilaments. Receptors were labeled with donor- or acceptor-conjugated Fab fragments, whereas microfilaments were labeled with acceptor- or donor-conjugated phalloidin reagents. Energy transfer was imaged by optical microscopy. We report that cell surface receptors can be constitutively unlinked, linked or inducibly linked to microfilaments.
authors.affiliation: Department of Biological Sciences, Wayne State University, Detroit, MI 48202.
authors.forename: M J
authors.initials: MJ
authors.lastname: Zhou
authors.forename: R F
authors.initials: RF
authors.lastname: Todd
authors.forename: H R
authors.initials: HR
authors.lastname: Petty
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000595:Amino Acid Sequence; D004735:Energy Transfer; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000378:metabolism*; D008853:Microscopy / Q000379:methods; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000378:metabolism* / Q000648:ultrastructure; D011951:Receptors, Complement / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000378:metabolism*
chemical_list: D007140:Immunoglobulin Fab Fragments; D011951:Receptors, Complement; D011971:Receptors, Immunologic