[Diagnosis of Fanconi's anemia by nitrogen mustard induction of chromosome breakage in fibroblasts].
Induction by nitrogen mustard of chromosome breakage in fibroblasts is proposed as an antenatal test for the diagnosis of Fanconi syndrome. After trypsinization of fibroblasts, addition to the culture medium of 0.025 micrograms/ml nitrogen mustard significantly increased chromosomal breaks in cells from patients with Fanconi syndrome but not in cells from controls. This protocol may prove useful for the antenatal diagnosis of Fanconi syndrome.
Desangles
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Laboratoire de Biologie Clinique, Hôpital du Val-de-Grâce, Paris, France.
D002869:Chromosome Aberrations / Q000175:diagnosis*; D025063:Chromosome Disorders; D002875:Chromosomes / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005199:Fanconi Anemia / Q000175:diagnosis* / Q000235:genetics; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D008466:Mechlorethamine / Q000494:pharmacology; D011296:Prenatal Diagnosis; D012016:Reference Values
D008466:Mechlorethamine