An inhibitor(s) of globin mRNA translation in rabbit serum.
1. A factor found in rabbit serum inhibits globin mRNA translation in vitro. 2. Inhibition of globin mRNA translation has been demonstrated in a cell-free rabbit reticulocyte lysate. 3. The inactivation of globin mRNA translation is not attributed to either serum albumin or ribonuclease activities. 4. Dialyzing the inhibitor for 24 hr at 4 degrees C does not result in the diminution of the inhibiting activity. However, the activity of the inhibitor is destroyed by heating to 70-80 degrees C for 5 min or by treatment with trypsin for 2 hr. 5. Ion exchange chromatography points to the inhibitor being a neutral protein, whereas, polyacrylamide gel electrophoresis reveals one major band with mol. wt 43 kDa. 6. The activity of the inhibiting material 3-fold greater in anemic serum than in normal serum. 7. These studies suggest that rabbit serum contains a protein inhibitor that may play a physiological role in regulating protein synthesis in red cells.
Rubin
H N
HN
Structural RNA, San Diego, CA 92121.
Stefanko
R S
RS
Halim
M N
MN
D000740:Anemia / Q000378:metabolism; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D002474:Cell-Free System; D002852:Chromatography, Ion Exchange; D005914:Globins / Q000235:genetics*; D007700:Kinetics; D008970:Molecular Weight; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits; D012260:Ribonucleases / Q000378:metabolism; D012709:Serum Albumin / Q000502:physiology; D013696:Temperature; D014357:Trypsin / Q000378:metabolism
D001798:Blood Proteins; D012333:RNA, Messenger; D012709:Serum Albumin; D005914:Globins; D012260:Ribonucleases; D014357:Trypsin