Cytoplasmic tail deletion converts membrane immunoglobulin to a phosphatidylinositol-linked form lacking signaling and efficient antigen internalization functions.
Membrane-bound immunoglobulin (mIg) is the antigen receptor on B lymphocytes mediating early events in antigen presentation and signal transduction. Wild-type human mIgM constructs transfected into the murine B-cell lymphoma A20 are expressed as transmembrane proteins with antigen presentation and signaling functions comparable to the endogenous mIgG2A; the transfected wild-type mIgM is internalized rapidly after anti-Ig cross-linking. Transfected constructs lacking the normal three-amino acid cytoplasmic tail are expressed exclusively as phosphatidylinositol-linked proteins, lack both antigen presentation and signal transduction functions, and are internalized slowly following anti-Ig binding. The molecular mass of the cytoplasmic tail-deleted phosphatidylinositol-linked Ig molecule is consistent with cleavage of the transmembrane residues during processing. Cytoplasmic domains may therefore regulate the mode of expression of membrane proteins and thereby influence their functional capabilities.
Mitchell
R N
RN
Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115.
Shaw
A C
AC
Weaver
Y K
YK
Leder
P
P
Abbas
A K
AK
D000818:Animals; D000941:Antigens / Q000378:metabolism; D003001:Cloning, Molecular; D004705:Endocytosis; D006801:Humans; D007075:Immunoglobulin M / Q000502:physiology*; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000502:physiology*; D051379:Mice; D010716:Phosphatidylinositols / Q000378:metabolism*; D011947:Receptors, Antigen, B-Cell / Q000502:physiology*; D011994:Recombinant Proteins; D015398:Signal Transduction; D013329:Structure-Activity Relationship
D000941:Antigens; D007075:Immunoglobulin M; D008565:Membrane Proteins; D010716:Phosphatidylinositols; D011947:Receptors, Antigen, B-Cell; D011994:Recombinant Proteins