DNA-ploidy and survival in gastric carcinomas: a flow-cytometric study.
In 125 gastric carcinomas the nuclear DNA content was determined by flow cytometry from formalin-fixed and paraffin-embedded tissue of surgical specimens. The carcinomas were of intestinal or mixed type (85), and diffuse type (40). DNA-aneuploidy was found in 46% of the intestinal type and in 42% of the mixed type, but only in 15% of the diffuse-type carcinomas (P less than 0.01). The total rate of DNA-aneuploidy was 34%. Carcinomas localized in the cardia were more frequently DNA-aneuploid than tumours in other localizations (P less than 0.01). DNA-aneuploid carcinomas had metastasized more frequently to regional lymph nodes (P less than 0.05) whereas no correlations with tumour stage and cytological/histological grade were detected. In 94 patients follow-up data were available. DNA-aneuploidy was associated with a statistically significant poorer prognosis when compared to DNA-diploid tumours only in advanced gastric carcinomas with lymph node metastases (P = 0.0488) and in the subgroup of advanced intestinal and mixed-type tumours (P = 0.0289).
Baretton
G
G
Institute of Pathology, Medical University of Lübeck, Federal Republic of Germany.
Carstensen
O
O
Schardey
M
M
Löhrs
U
U
D000230:Adenocarcinoma / Q000145:classification / Q000235:genetics* / Q000401:mortality; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000782:Aneuploidy; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008223:Lymphoma / Q000737:chemistry / Q000235:genetics; D008297:Male; D008875:Middle Aged; D011003:Ploidies; D011379:Prognosis; D013274:Stomach Neoplasms / Q000145:classification / Q000235:genetics* / Q000401:mortality; D016019:Survival Analysis
D004273:DNA, Neoplasm