Clinical relevance of the quantification of apolipoprotein E in cerebrospinal fluid.
Apolipoprotein E was measured in paired sera and cerebrospinal fluid samples from 483 neurological patients. The average apolipoprotein E concentration was 7.5 (+/- 3.1) mg/l in cerebrospinal fluid and 93.5 (+/- 29.7) mg/l in serum. Mean apolipoprotein B concentrations in 88 patients were 0.77 +/- 3.4 mg/l in cerebrospinal fluid and 1.06 +/- 0.31 g/l in serum. The apolipoprotein E concentration in cerebrospinal fluid was much greater than expected for passive diffusion from serum. By contrast to apolipoprotein B, there was no correlation between the apolipoprotein E cerebrospinal fluid/serum concentration quotient and the albumin concentration quotient. This suggests that apolipoprotein E in cerebrospinal fluid, unlike apolipoprotein B, is locally synthesized and that the use of a cerebrospinal fluid/serum concentration quotient is unnecessary. In a control group (n = 64) the mean cerebrospinal fluid apolipoprotein E value (+/- SD) was 5.9 (1.6) mg/l. Elevated cerebrospinal fluid apolipoprotein E concentrations were observed in acute (n = 22, 12.5 +/- 6.2 mg/l) and chronic inflammatory central nervous system diseases (n = 15, 10.4 +/- 2.4 mg/l).
Carlsson
J
J
Department of Clinical Chemistry, University Hospital Göttingen, FRG.
Armstrong
V W
VW
Reiber
H
H
Felgenhauer
K
K
Seidel
D
D
D000328:Adult; D000418:Albumins / Q000134:cerebrospinal fluid; D001055:Apolipoproteins B / Q000134:cerebrospinal fluid; D001057:Apolipoproteins E / Q000097:blood / Q000134:cerebrospinal fluid*; D002555:Cerebrospinal Fluid / Q000737:chemistry / Q000166:cytology; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D002784:Cholesterol / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid; D008297:Male; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid / Q000175:diagnosis; D009422:Nervous System Diseases / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D009443:Neuritis / Q000134:cerebrospinal fluid / Q000175:diagnosis; D014280:Triglycerides / Q000097:blood; D014461:Ultracentrifugation
D000418:Albumins; D001055:Apolipoproteins B; D001057:Apolipoproteins E; D002556:Cerebrospinal Fluid Proteins; D007074:Immunoglobulin G; D014280:Triglycerides; D002784:Cholesterol