Reactive blue 2 is a potent inhibitor of a thylakoid protein kinase.
The anthraquinone dye reactive blue 2 was found to be a potent inhibitor of a protein kinase isolated and purified from thylakoids. This enzyme was also inhibited in situ, with corresponding inhibition of ATP-dependent quenching of the chlorophyll fluorescence. The mode of inhibition was noncompetitive, with a Ki of 8 microM for the membrane-bound kinase, and 6 microM for the purified kinase. The inhibitor did not modify the substrate preference of the endogenous kinase and could be removed from the membrane by washing. Unlike reactive blue 2, the enzyme did not partition into detergent micelles and is therefore presumably not a hydrophobic, intrinsic membrane protein.
Coughlan
S J
SJ
Biology Department Brookhaven National Laboratory, Upton, NY 11973.
Davenport
J W
JW
Hind
G
G
D000255:Adenosine Triphosphate / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D004396:Coloring Agents; D004591:Electrophoresis, Polyacrylamide Gel; D005453:Fluorescence; D007425:Intracellular Membranes / Q000201:enzymology; D007700:Kinetics; D010766:Phosphorylation; D010944:Plants / Q000201:enzymology*; D047428:Protein Kinase Inhibitors; D013501:Surface-Active Agents; D014227:Triazines / Q000494:pharmacology*
D004396:Coloring Agents; D047428:Protein Kinase Inhibitors; D013501:Surface-Active Agents; D014227:Triazines; D002734:Chlorophyll; C012842:Cibacron Blue F 3GA; D000255:Adenosine Triphosphate