Local inhibition of thrombosis using urokinase linked to a monoclonal antibody which recognises damaged endothelium.
A monoclonal antibody which binds to damaged endothelial cells was covalently linked to urokinase in an attempt to produce a thrombolytic agent which could be localised to areas of endothelial damage. The conjugate was shown to be effective in inducing localised thrombolysis in vitro, and in a rat vena cava thrombosis model brief exposure of the intimal surface of the isolated vena cava to the antibody -urokinase conjugate prevented or reduced subsequent stasis-induced thrombosis without inducing a systemic lytic state.
de Bono
D P
DP
Department of Cardiology, University of Leicester, Gienfield General Hospital Leicester, England.
Pringle
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D004337:Drug Carriers; D004730:Endothelium, Vascular / Q000276:immunology / Q000293:injuries; D005260:Female; D005342:Fibrinolysis / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013927:Thrombosis / Q000188:drug therapy*; D014568:Urokinase-Type Plasminogen Activator / Q000008:administration & dosage*; D014684:Venae Cavae / Q000293:injuries
D000911:Antibodies, Monoclonal; D004337:Drug Carriers; D014568:Urokinase-Type Plasminogen Activator