Calcium binding proteins as molecular markers for cat geniculate neurons.
Immunocytochemistry revealed that in the cat dorsal lateral geniculate nucleus (dLGN) almost all parvalbumin-positive cells are GABAergic and about 56% of the calbindin D-28K (calbindin-immunoreactive neurons are also GABA-positive. On the other hand, in the same nucleus, almost all GABAergic neurons contain parvalbumin, and about 89% of the GABA-immunoreactive neurons contain calbindin. Double-labeling with calbindin and parvalbumin revealed that approximately 50% of the immunoreactive neurons are double-stained. In the PGN, virtually all neurons are GABA and parvalbumin-positive. Only a few scattered cells were also calbindin-immunoreactive. These results show that GABAergic geniculate cells can be differentiated on the basis of their calcium-binding protein immunoreactivity. Four types of immunoreactive cells are described here: (1) cells positive for GABA, parvalbumin and calbindin, (2) cells positive for GABA and parvalbumin, but negative for calbindin, (3) cells negative for GABA and parvalbumin, but positive for calbindin, (4) cells negative for GABA, parvalbumin and calbindin.
Demeulemeester
H
H
Laboratory of Neuroendocrinology, Catholic University Leuven, Belgium.
Arckens
L
L
Vandesande
F
F
Orban
G A
GA
Heizmann
C W
CW
Pochet
R
R
D000818:Animals; D015415:Biomarkers; D064026:Calbindins; D002135:Calcium-Binding Proteins / Q000032:analysis*; D002415:Cats; D005829:Geniculate Bodies / Q000166:cytology*; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D009474:Neurons / Q000166:cytology*; D010320:Parvalbumins / Q000032:analysis*; D064030:S100 Calcium Binding Protein G / Q000032:analysis*; D005680:gamma-Aminobutyric Acid / Q000032:analysis
D015415:Biomarkers; D064026:Calbindins; D002135:Calcium-Binding Proteins; D007106:Immune Sera; D010320:Parvalbumins; D064030:S100 Calcium Binding Protein G; D005680:gamma-Aminobutyric Acid