title: Characterization of carbohydrates linked to rubella virus glycoprotein E2.
abstract: Rubella virus contains two envelope glycoproteins, E1 and E2. The amino acid sequence for both glycoproteins is known, as is the number of N-glycosylation sites. This study has demonstrated the presence of O-linked carbohydrates bound to E2 and determined structural characteristics of the N-linked oligosaccharide chains. O-linked sugars were found to be resistant to digestion with N-glycanase but sensitive to beta-elimination with alkaline borohydride. After treatment with neuraminidase, O-linked sugars bound to peanut agglutinin, suggesting the presence of the disaccharide galactose-N-acetylgalactosamine, masked by sialic acid. The N-linked oligosaccharides were large, probably four-branched, and showed a lectin binding pattern suggesting the complex type, with terminal Gal, GlcNAc and sialic acid. No Endo H-sensitive carbohydrates were detected.
authors.affiliation: Department of Paediatrics and Pathology, University of British Columbia, Vancouver, Canada.
authors.forename: M L
authors.initials: ML
authors.lastname: Lundström
authors.forename: C A
authors.initials: CA
authors.lastname: Mauracher
authors.forename: A J
authors.initials: AJ
authors.lastname: Tingle
mesh_terms: D000581:Amidohydrolases; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006020:Glycopeptides / Q000302:isolation & purification; D006026:Glycoside Hydrolases; D006031:Glycosylation; D037102:Lectins; D008970:Molecular Weight; D009844:Oligosaccharides / Q000302:isolation & purification*; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D010748:Phosphopeptides / Q000302:isolation & purification; D012412:Rubella virus / Q000378:metabolism*; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D002241:Carbohydrates; D006020:Glycopeptides; D037102:Lectins; D009844:Oligosaccharides; D010748:Phosphopeptides; D014759:Viral Envelope Proteins; C061827:E2 envelope protein, Rubella virus; D006026:Glycoside Hydrolases; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase