Erythrocyte defense mechanisms against free oxygen radicals in haemodialysed uraemic children.
Changes in erythrocyte lipid peroxidation (measured as the concentration of malonyl dialdehyde), glutathione metabolism, antioxidant enzyme activities (glutathione peroxidase, catalase, and superoxide dismutase), the oxidized products of haemoglobin (Hb), and hydrogen peroxide (H2O2)-induced haemolysis were studied in six children with chronic renal failure treated with serial acetate and bicarbonate haemodialysis (HD). Ten age- and sex-matched children acted as controls. Malonyl dialdehyde levels were significantly higher and antioxidant enzyme activities lower in uraemic red blood cells (RBCs) compared with controls (P less than 0.05). Incubation of RBCs for 1 h with acetylphenylhydrazine induced a decrease in the concentration of reduced glutathione (P less than 0.001) and an increase in the level of oxidized products of Hb (P less than 0.001), but only in the uraemic patients. The H2O2 haemolysis test revealed a mild (n = 3) to increased (n = 3) haemolysis in the uraemic RBCs. Oxidative haemolysis is probably a multifactorial process in uraemic patients, and may be an important risk factor in HD therapy.
Túri
S
S
Department of Paediatrics, Albert Szent-Györgyi Medical University, Szeged, Hungary.
Németh
I
I
Vargha
I
I
Matkovics
B
B
Dobos
E
E
D000293:Adolescent; D000743:Anemia, Hemolytic / Q000209:etiology* / Q000503:physiopathology; D002648:Child; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D005609:Free Radicals; D005978:Glutathione / Q000097:blood; D006801:Humans; D007676:Kidney Failure, Chronic / Q000503:physiopathology / Q000628:therapy*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D010100:Oxygen; D006435:Renal Dialysis / Q000009:adverse effects*; D014511:Uremia / Q000503:physiopathology / Q000628:therapy*
D005609:Free Radicals; D008315:Malondialdehyde; D005978:Glutathione; D010100:Oxygen