The U1 snRNA gene repeat from the sea urchin (Strongylocentrotus purpuratus): the 70 kilobase tandem repeat ends directly 3' to a U1 gene.
Lambda phage clones containing multiple copies of the 1.1 kb tandemly repeated unit of the sea urchin (S. purpuratus) U1 RNA genes were isolated from a gene library. The 1.1 kb repeat unit encodes a single copy of the predominant U1 RNA expressed in oocytes and embryos prior to the blastula stage. The tandem repeat unit is about 80 kb in size and is probably present one time per haploid genome as judged by pulsed-field electrophoresis of sperm DNA digested with restriction enzymes which do not cut in the repeat unit. Two of the phage contained DNA flanking the repeat unit as well as several repeat units. The tandem repeat unit ends just 3' to the U1 coding region. There is only limited homology in the 5' flanking region with U1 snRNA genes from the sea urchin L. variegatus.
Yu
J C
JC
Department of Chemistry, Florida State University, Tallahassee 32306.
Wendelburg
B
B
Sakallah
S
S
Marzluff
W F
WF
D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D004247:DNA; D015723:Gene Library; D008969:Molecular Sequence Data; D005810:Multigene Family; D012342:RNA, Small Nuclear / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D012617:Sea Urchins; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D012342:RNA, Small Nuclear; D004247:DNA