The interaction between halogenated anaesthetics and bacteriorhodopsin in purple membranes as examined by intrinsic ultraviolet fluorescence.
In the presence of halogenated general anaesthetics such as enflurane and halothane, the spectral properties of the bacteriorhodopsin pigment contained in the purple membranes of Halobacterium halobium are strongly modified. It is reversibly transformed into a red-coloured species absorbing maximally at 480 nm, at the expense of its characteristic 570-nm absorption band. The ultraviolet fluorescence of bacteriorhodopsin has been used to probe the structural modifications that are reflected by this spectral change. Our results show that they are very small and do not perturb the energy transfer dynamics which take place between the aromatic amino acid residues and the retinyl chromophore. The fluorescence properties of anaesthetic-treated bacteriorhodopsin are dominated by the quenching properties of the halogenated hydrocarbon, which are obvious even at anaesthetic concentrations under those needed to induce a spectral change in the bacteriorhodopsin chromophore. This does not rule out direct interaction between anaesthetics and bacteriorhodopsin, but it indicates that the chromophoric site might well not be their primary target.
Lee
K H
KH
Centre de recherche en photobiophysique, Université du Québec à Trois-Rivières, Canada.
McIntosh
A R
AR
Boucher
F
F
D001436:Bacteriorhodopsins / Q000737:chemistry* / Q000378:metabolism; D001965:Bromides / Q000737:chemistry / Q000378:metabolism; D004737:Enflurane / Q000737:chemistry* / Q000378:metabolism; D004986:Ether / Q000737:chemistry / Q000378:metabolism; D006217:Halobacterium / Q000032:analysis*; D006221:Halothane / Q000737:chemistry* / Q000378:metabolism; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D012964:Sodium / Q000737:chemistry / Q000378:metabolism; D017670:Sodium Compounds; D013050:Spectrometry, Fluorescence
D001965:Bromides; D046911:Macromolecular Substances; D008565:Membrane Proteins; D017670:Sodium Compounds; D004986:Ether; D001436:Bacteriorhodopsins; D004737:Enflurane; D012964:Sodium; C027938:sodium bromide; D006221:Halothane