Characterization of anti-endothelial cell antibodies in systemic lupus erythematosus (SLE).
IgG anti-endothelial antibodies (AEA), as measured by ELISA or immunoblotting technique could be detected in serum samples of 56 out of 64 patients with SLE (88%) and mainly occurred in monomeric form. AEA were not cell specific, because the binding reactivity was absorbed partially by both fibroblasts and peripheral blood mononuclear cells. No correlation was found between the presence of AEA and anti-nuclear antibodies. Immunoblotting revealed reactivity of AEA against endothelial antigens ranging in size from 15 to 200 kD. AEA titres were significantly higher in patients with joint or skin abnormalities, compared with patients without these abnormalities. A significant correlation was found between nephritis in SLE and the presence of AEA reactivity against endothelial membrane antigens of 38, 41 and 150 kD. These data show that the pattern of AEA reactivity in serum of SLE patients is heterogeneous, and suggest that AEA against a limited number of antigens may be involved in the pathogenesis of nephritis in SLE.
van der Zee
J M
JM
Department of Rheumatology, University Hospital, Leiden, The Netherlands.
Siegert
C E
CE
de Vreede
T A
TA
Daha
M R
MR
Breedveld
F C
FC
D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D015153:Blotting, Western; D015922:Complement C1q / Q000032:analysis; D003176:Complement C3 / Q000032:analysis; D003181:Complement C4 / Q000032:analysis; D015941:Complement Hemolytic Activity Assay; D004730:Endothelium, Vascular / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007592:Joint Diseases / Q000276:immunology; D007674:Kidney Diseases / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D009422:Nervous System Diseases / Q000276:immunology; D012700:Serositis / Q000276:immunology; D012871:Skin Diseases / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D003176:Complement C3; D003181:Complement C4; D007074:Immunoglobulin G; D015922:Complement C1q