Molecular cloning of the 5-aminolevulinic acid dehydratase gene from Rhodobacter sphaeroides.
A hemB mutant of Escherichia coli was used to clone the gene encoding 5-aminolevulinic acid dehydratase from Rhodobacter sphaeroides after physiological complementation of the mutation. A 2.9-kb DNA fragment was obtained and cloned in both orientations into the unique PstI restriction site of pUC19. This recombinant plasmid encodes a protein (Mr 39,000) that is immunoreactive with antibodies raised against the enzyme from higher plants.
Delaunay
A M
AM
Centre National de la Recherche Scientifique, Unité Associée 203, Faculté des Sciences, Mont Saint Aignan, France.
Huault
C
C
Balangé
A P
AP
D015139:Blotting, Southern; D015153:Blotting, Western; D002374:Catalase / Q000096:biosynthesis; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D010957:Plasmids / Q000235:genetics; D000623:Porphobilinogen Synthase / Q000096:biosynthesis / Q000235:genetics*; D011995:Recombination, Genetic; D012242:Rhodobacter sphaeroides / Q000235:genetics*
D004247:DNA; D002374:Catalase; D000623:Porphobilinogen Synthase