Modulatory effects of progesterone upon dopamine release from the corpus striatum of ovariectomized estrogen-treated rats are stereo-specific.
We have shown previously that progesterone linked with bovine serum albumin at position 3 of the progesterone molecule (P-3-BSA) was effective in modulating (both stimulating and inhibiting) amphetamine (AMPH)-evoked dopamine (DA) release in vitro from superfused corpus striatal (CS) tissue fragments of ovariectomized estrogen-primed rats. To examine the stereospecificity of this effect, progesterone linked with bovine serum albumin at position 11 (P-11-BSA) and deoxycorticosterone linked with bovine serum albumin at position 21 (DOC-BSA) were tested for their ability to modulate amphetamine-stimulated DA release in vitro when administered in either a pulsatile or a continuous infusion mode at different doses. The results demonstrate that a specific orientation of the P-BSA molecule is required for differential modulation of AMPH stimulated DA release from the CS of ovariectomized estrogen-treated rats, since DOC-BSA was ineffective and P-11-BSA showed only a stimulatory effect.
Dluzen
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Department of Physiology and Biophysics, University of Illinois, Urbana 61801.
Ramirez
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D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects / Q000502:physiology*; D003913:Dextroamphetamine / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism*; D004334:Drug Administration Schedule; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D007262:Infusions, Intravenous; D010052:Ovariectomy; D011374:Progesterone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology*; D051381:Rats; D012709:Serum Albumin / Q000494:pharmacology*; D012710:Serum Albumin, Bovine; D013329:Structure-Activity Relationship
D012709:Serum Albumin; C075151:progesterone 3-(O-carboxymethyl)oxime-bovine serum albumin; C068561:progesterone-11-bovine serum albumin; D012710:Serum Albumin, Bovine; D011374:Progesterone; D004958:Estradiol; D003913:Dextroamphetamine; D004298:Dopamine