The osmotic responses of Saccharomyces cerevisiae in K(+)-depleted medium.
Saccharomyces cerevisiae showed reduced growth in a K(+)-depleted medium but was still capable of synthesizing and accumulating glycerol in the presence of 2.28% (w/v) NaCl; glycerol levels were similar in both K(+)-replete and K(+)-depleted media. The activity of glycerol-3-phosphate dehydrogenase, a key enzyme of glycerol synthesis, was inhibited to a similar extent by exogenous K+ and Na+ although such effects may be modified in vivo due to ionic compartmentation within the vacuole. These results indicate that exogenous K+ does not play a significant role in the initiation of glycerol synthesis in osmotically-stressed yeast.
Meikle
A J
AJ
Department of Biological Sciences, University of Dundee, Scotland, U.K.
Reed
R H
RH
Gadd
G M
GM
D005990:Glycerol / Q000378:metabolism*; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D007700:Kinetics; D011188:Potassium / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000254:growth & development / Q000502:physiology*; D012964:Sodium / Q000378:metabolism; D014882:Water-Electrolyte Balance
D012964:Sodium; D005993:Glycerolphosphate Dehydrogenase; D005990:Glycerol; D011188:Potassium