title: Structural analysis of biologically active peptides and recombinant proteins and their modified counterparts by mass spectrometry.
abstract: The structural characterization of the Escherichia coli-expressed human interferon alpha-2b (rh-IFN alpha-2b) was carried out by employing the fast atom bombardment (FAB) and plasma desorption (PD) mapping methods. The mass spectral data of the rh-IFN alpha-2b and the trypsin-generated peptide mixture allowed rapid and facile confirmation of the cDNA-derived sequence and determination of the existing disulfide pattern in the protein molecule. The same PD/FAB mapping approach was successfully employed in the structural determination of the iodination reaction product of rh-IFN alpha-2b and the potent vasoconstrictor peptide endothelin.
authors.affiliation: Schering-Plough Research, Bloomfield, NJ 07003.
authors.forename: B N
authors.initials: BN
authors.lastname: Pramanik
authors.forename: A
authors.initials: A
authors.lastname: Tsarbopoulos
authors.forename: J E
authors.initials: JE
authors.lastname: Labdon
authors.forename: P P
authors.initials: PP
authors.lastname: Trotta
authors.forename: T L
authors.initials: TL
authors.lastname: Nagabhushan
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003545:Cysteine / Q000737:chemistry; D016232:Endothelins / Q000032:analysis / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000032:analysis; D006801:Humans; D006868:Hydrolysis; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000032:analysis; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis*; D011994:Recombinant Proteins / Q000032:analysis*; D012697:Serine Endopeptidases; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014357:Trypsin
chemical_list: D016232:Endothelins; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D010455:Peptides; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D014357:Trypsin; D003545:Cysteine