title: Carcinoma-in-situ cells in cultured human seminiferous tubules.
abstract: For the first time, early germ-cell derived tumour cells were studied in an in-vitro system of cultured seminiferous tubules. The intratubular tumour cells not only survived in culture for 7 days but were also able to multiply. Dividing tumour cells were identified in semi-thin sections and electron micrographs by morphological criteria. Additionally, mitotic activity was demonstrated by [3H]thymidine histo-autoradiography. There are numerous reports on cell lines established from solid non-seminomas, but up to now no references to seminoma cell lines or cultures of intratubular tumour cells are available. The culture of seminiferous tubules offers a tool in making carcinoma-in-situ cells accessible for experimental work.
authors.affiliation: Department of Microscopical Anatomy, University of Hamburg, FRG.
authors.forename: H
authors.initials: H
authors.lastname: Lauke
authors.forename: K
authors.initials: K
authors.lastname: Seidl
authors.forename: M
authors.initials: M
authors.lastname: Hartmann
authors.forename: A F
authors.initials: AF
authors.lastname: Holstein
mesh_terms: D001345:Autoradiography; D002278:Carcinoma in Situ / Q000473:pathology; D004407:Dysgerminoma; D006801:Humans; D008297:Male; D012671:Seminiferous Tubules / Q000473:pathology; D013724:Teratoma; D013736:Testicular Neoplasms; D014407:Tumor Cells, Cultured