Chromosomal in situ suppression hybridization after Giemsa banding.
We report the successive application of classical Giemsa banding and chromosomal in situ suppression hybridization in clinical cytogenetics. The use of both techniques within one protocol requires an additional fixation of the chromosome preparations and an improved suppression of the labelled repetitive sequences. The combination of these two cytological techniques allows the high resolution mapping of translocated Y-chromosomal sequences in the chromosome set of an XX-male.
Klever
M
M
Institut für Humangenetik und Anthropologie der Universität, Heidelberg, Federal Republic of Germany.
Grond-Ginsbach
C
C
Scherthan
H
H
Schroeder-Kurth
T M
TM
D002871:Chromosome Banding / Q000379:methods*; D006801:Humans; D007231:Infant, Newborn; D007621:Karyotyping / Q000379:methods*; D008297:Male; D009693:Nucleic Acid Hybridization; D014178:Translocation, Genetic; D014960:X Chromosome; D014998:Y Chromosome