title: Nonradioactive in situ hybridization. A rapid approach for the identification of marker chromosomes: study of a case of acute leukemia with a Yq specific DNA probe.
abstract: Nonradioactive in situ hybridization provides a rapid method for detecting specific nucleic acid sequences. In this study of a patient with acute leukemia, we applied in situ hybridization for identification of a marker chromosome and determination of the number of copies of this marker in interphase nuclei using a biotinylated Yq-specific DNA probe (pY3.4). We show that nonradioactive interphase in situ hybridization can be a useful method for karyotypic analysis in addition to routine cytogenetic techniques in neoplastic disorders.
authors.affiliation: Cancer Center of Southwest Biomedical Research Institute and Genetrix, Inc., Scottsdale, AZ 85251.
authors.forename: B
authors.initials: B
authors.lastname: Wullich
authors.forename: R
authors.initials: R
authors.lastname: Morgan
authors.forename: C
authors.initials: C
authors.lastname: Berger
authors.forename: V
authors.initials: V
authors.lastname: Jarzabek
authors.forename: A A
authors.initials: AA
authors.lastname: Sandberg
mesh_terms: D000208:Acute Disease; D000368:Aged; D002871:Chromosome Banding; D015342:DNA Probes; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D007938:Leukemia / Q000235:genetics*; D008297:Male; D008677:Metaphase; D009693:Nucleic Acid Hybridization; D014998:Y Chromosome
chemical_list: D015342:DNA Probes; D005819:Genetic Markers