Acidic glycosphingolipids of human amnion.
Six major acidic glycosphingolipids were isolated from human amnion using DEAE Sephadex A-25 and silica beads column chromatography. The structures of these glycosphingolipids were determined by methylation analysis, TLC immunostaining and/or negative ion FAB-MS, and were concluded to be II3 alpha NeuAcLacCer(GM3), IV3 alpha NeuAcnLc4-Cer (sialyl[alpha 2-3]paragloboside), IV6 alpha NeuAcnLc4Cer (sialyl[alpha 2-6]paragloboside), IV3 alpha NeuAcIII4 alpha FucLc4Cer (sialyl Lea), VI3 alpha NeuAcnLc6Cer (i-ganglioside) and II3 alpha (NeuAc alpha 2----8NeuAc)LacCer (GD3). In addition, several minor glycosphingolipids were detected with specific monoclonal antibodies, including glycolipids with NeuAc alpha 2-3Gal beta 1-4GlcNAc-beta 1- or NeuAc alpha 2-6Gal beta 1-4GlcNAc beta 1- determinant. Our results show that the glycosphingolipids of human amnion are characterized by having mainly type II chain analogues and onco-fetal antigens.
Asano
K
K
Division of Clinical Biochemistry, National Medical Center, Tokyo.
Suzuki
M
M
Handa
S
S
Oshima
M
M
D000650:Amnion / Q000737:chemistry*; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000737:chemistry / Q000302:isolation & purification; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005260:Female; D006028:Glycosphingolipids / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D011247:Pregnancy
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D006028:Glycosphingolipids; C031192:oncofetal antigens