title: Isolation and characterization of phosmidosine. A new antifungal nucleotide antibiotic.
abstract: A new nucleotide antibiotic, phosmidosine was isolated from a culture filtrate of a newly isolated streptomycete identified as Streptomyces sp. RK-16. HRFAB-MS and elemental analysis established the molecular formula of C16H24N7O8P. 1H, 13C and 31P NMR indicated the presence of a methyl phosphate group and UV spectra were similar to those of 8-hydroxyadenosine. The antibiotic inhibited spore formation of Botrytis cinerea at the concentration of 0.25 micrograms/ml.
authors.affiliation: Antibiotics Laboratory, RIKEN, Institute of Physical and Chemical Research, Saitama, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Uramoto
authors.forename: C J
authors.initials: CJ
authors.lastname: Kim
authors.forename: K
authors.initials: K
authors.lastname: Shin-Ya
authors.forename: H
authors.initials: H
authors.lastname: Kusakabe
authors.forename: K
authors.initials: K
authors.lastname: Isono
authors.forename: D R
authors.initials: DR
authors.lastname: Phillips
authors.forename: J A
authors.initials: JA
authors.lastname: McCloskey
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D003904:Mitosporic Fungi / Q000187:drug effects / Q000502:physiology; D015394:Molecular Structure; D011685:Purine Nucleotides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013172:Spores, Fungal / Q000187:drug effects; D013302:Streptomyces / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D011685:Purine Nucleotides; C068885:phosmidosine