Distribution of free and conjugated catecholamines between plasma, platelets and erythrocytes: different effects of intravenous and oral catecholamine administrations.
Plasma, platelet and erythrocyte contents of free and conjugated norepinephrine, epinephrine and dopamine were determined by radioenzymatic assay in 12 resting healthy volunteers. Mean platelet/plasma concentration ratios were 533 for free norepinephrine, 502 for free epinephrine and 149 for free dopamine. Corresponding erythrocyte/plasma ratios were 1.04, 1.13 and 4.5, respectively. The presence of conjugated catecholamines in platelets and erythrocytes could be confirmed; however, their relative proportion within these cells, particularly in platelets, was lower than that in plasma. Upon intravenous infusion of dopamine for 3 hr at 5 micrograms kg-1 min-1, concentrations of free dopamine in plasma increased rapidly (280-970-fold), whereas conjugated dopamine only reached maximal values (14-19-fold increase) at 30 to 60 min after cessation of the infusion. The relative distribution of unconjugated dopamine in whole blood between plasma, platelets and erythrocytes changed from mean values of 1:0.33:3.7 at rest to 1:1.1:0.5 at the end of the infusion. As a result of the subsequent rapid decrease of dopamine in plasma and erythrocytes, this distribution was 1:17:1 shortly thereafter and remained constant up to the end of the investigation period. The relative distribution for conjugated dopamine of 1:0.001:0.5 at rest changed to about 1:0.2:0.1 at the termination of the infusion. Oral administration of norepinephrine and dopamine led to increases in the plasma concentrations of these amines in their conjugated forms only, whereas epinephrine concentrations remained constant. These elevations were not accompanied by corresponding increases in platelet and erythrocyte norepinephrine, epinephrine and dopamine contents.(ABSTRACT TRUNCATED AT 250 WORDS)
Ratge
D
D
Department of Clinical Chemistry, Robert-Bosch-Hospital, Stuttgart Federal Republic of Germany.
Kohse
K P
KP
Steegmüller
U
U
Wisser
H
H
D000284:Administration, Oral; D000328:Adult; D001792:Blood Platelets / Q000378:metabolism*; D002395:Catecholamines / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D004298:Dopamine / Q000031:analogs & derivatives* / Q000097:blood; D004837:Epinephrine / Q000031:analogs & derivatives* / Q000097:blood; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006801:Humans; D007275:Injections, Intravenous; D008297:Male; D009638:Norepinephrine / Q000031:analogs & derivatives* / Q000097:blood
D002395:Catecholamines; C022807:dopamine 4-O-sulfate; C007558:dopamine 3-O-sulfate; C036526:epinephrine sulfate; C017574:noradrenaline sulfate; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine