Radionuclide angiography with technetium-99m in vivo labeled erythrocytes does not lead to induction of mutations in the HPRT gene of human T-lymphocytes.
Mutant frequencies were measured in T-lymphocytes of patients undergoing radionuclide angiography with erythrocytes labeled in vivo with technetium-99m. Blood from 13 patients was sampled before and after (8-120 days) an injection with 750 MBq technetium-99m. Frequencies of HPRT- mutants were measured with the T-cell cloning method. Results indicated that the mean frequency of mutants after treatment was significantly below that measured before exposure. Thus, in contrast to published data, our results do not support the conclusion that radionuclide angiography with technetium-99m induces HPRT- mutations. Further analysis of our data indicated that the decrease in mutant frequency after exposure can be accounted for by an effect of cloning efficiency.
van Dam
F J
FJ
MGC-Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, The Netherlands.
Camps
J A
JA
Woldring
V M
VM
Natarajan
A T
AT
van der Wall
E E
EE
Zwinderman
A H
AH
Lohman
P H
PH
Pauwels
E K
EK
Tates
A D
AD
D000328:Adult; D000368:Aged; D004912:Erythrocytes; D005260:Female; D015637:Gated Blood-Pool Imaging; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D011831:Radiation Genetics; D013601:T-Lymphocytes / Q000528:radiation effects*; D013667:Technetium
D013667:Technetium; D007041:Hypoxanthine Phosphoribosyltransferase