title: Single procedure for detection, confirmation, and quantification of benzodiazepines in serum by liquid chromatography with photodiode-array detection.
abstract: We developed a reversed-phase chromatographic procedure for detecting benzodiazepines and other drugs in serum. A liquid-liquid extraction step with hexane/ethyl acetate isolates the drugs from serum; absolute recoveries are generally greater than 85%. Reconstituted extracts are chromatographed on a 4-microns (particle size) C18 column; 14 drugs and an internal standard (flunitrazepam) are separated in 8 min. Peak detection, purity checking, and identification are performed with a computerized photodiode-array detector. Run-to-run imprecision (CV) for many benzodiazepines is less than 3%. In a study of 126 specimens from Emergency Department patients, the procedure showed excellent agreement with a gas-chromatographic method involving either mass-spectrometric or flame-ionization detection. This single procedure provides rapid and accurate detection, quantification, and confirmation of benzodiazepines in serum.
authors.affiliation: Chemistry Laboratory, Massachusetts General Hospital, Boston 02114.
authors.forename: P R
authors.initials: PR
authors.lastname: Puopolo
authors.forename: M E
authors.initials: ME
authors.lastname: Pothier
authors.forename: S A
authors.initials: SA
authors.lastname: Volpicelli
authors.forename: J G
authors.initials: JG
authors.lastname: Flood
mesh_terms: D001569:Benzodiazepines / Q000097:blood*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D015813:Substance Abuse Detection
chemical_list: D001569:Benzodiazepines