Quantitative comparison of magnetic resonance imaging (MRI) and histologic analyses of focal ischemic damage in the rat.
Hemispheric swelling and area of infarction, two parameters of cerebral focal ischemic damage, were identified and quantified from T2-weighted magnetic resonance imaging (MRI) two days after occlusion of the middle cerebral artery (MCAO) in spontaneously hypertensive rat (SHR) brains. Results were compared with these measures quantified from 2,3,5-triphenyltetrazolium hydrochloride (TTC)- and hematoxylin and eosin (H&E)-stained histologic sections in the same brains. The degree of hemispheric swelling and infarct size determined by MRI were highly correlated to the measurements as determined in the TTC- and H&E-stained tissues. These results demonstrate that the focal ischemic damaged area and associated tissue swelling identified by MRI is quantitatively similar to, and thus, is representative of actual tissue damage/changes that can be identified by gross or histologic examination.
Barone
F C
FC
Department of Pharmacology, SmithKline Beecham Pharmaceuticals, plc, King of Prussia, PA 19406.
Clark
R K
RK
Feuerstein
G
G
Lenkinski
R E
RE
Sarkar
S K
SK
D000818:Animals; D002544:Cerebral Infarction / Q000175:diagnosis; D002546:Ischemic Attack, Transient / Q000175:diagnosis*; D008279:Magnetic Resonance Imaging; D051381:Rats; D011918:Rats, Inbred SHR