title: Preparation of human manganese superoxide dismutase by tri-phase partitioning and preliminary crystallographic data.
abstract: Human liver manganese superoxide dismutase has been purified by a short procedure that includes a tri-phase partitioning step to provide materials that can be crystallized from ammonium sulfate. X-ray diffraction studies at 3 A resolution show that the crystals belong to the hexagonal space group P6(1)22 or P6(5)22, with cell dimensions a = b = 81.1 A, c = 242.2 A. Manganese superoxide dismutase levels as determined by enzymatic assay as well as by enzyme-linked immunosorbent assay indicated that considerable variations occur in different livers but the total superoxide dismutase activity (Mn superoxide dismutase plus Cu,Zn superoxide dismutase) seems to be kept at constant values.
authors.affiliation: Department of Biochemistry, Osaka University Medical School, Japan.
authors.forename: H F
authors.initials: HF
authors.lastname: Deutsch
authors.forename: S
authors.initials: S
authors.lastname: Hoshi
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuda
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Kawano
authors.forename: Y
authors.initials: Y
authors.lastname: Kitagawa
authors.forename: Y
authors.initials: Y
authors.lastname: Katsube
authors.forename: N
authors.initials: N
authors.lastname: Taniguchi
mesh_terms: D000645:Ammonium Sulfate; D003460:Crystallization; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D011487:Protein Conformation; D013482:Superoxide Dismutase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D007527:Isoenzymes; D013482:Superoxide Dismutase; D000645:Ammonium Sulfate