Protection conferred on mice by monoclonal antibodies directed against outer-membrane-protein antigens of Brucella.
Twenty-six monoclonal antibodies (MAbs) directed against seven brucella outer-membrane proteins (OMPs) of 10, 16.5, 19, 25-27, 31-34, 36-38 and 89 Kda were screened for passive protection of mice; three MAbs (directed against 16.5, 25-27 and 36-38 Kda) reduced significantly the initial colonisation of the spleen measured 7 days after challenge. Although significant, the reduction in numbers of Brucella organisms in the spleen was low compared with that conferred by MAbs against lipopolysaccharide of smooth specificity (S-LPS). The three most protective MAbs belonged to two isotypes (IgG1 and IgG2a) and were specific for three different OMPs. No relationship between protection and binding of MAbs to the challenge S strain or its rough mutant was observed in the mouse model. The humoral protection depended mainly on antibodies directed against S-LPS, although some MAbs against OMPs had weak protective activity.
Cloeckaert
A
A
Unit of Experimental Medicine, Catholic University of Louvain, Brussels, Belgium.
Jacques
I
I
Bosseray
N
N
Limet
J N
JN
Bowden
R
R
Dubray
G
G
Plommet
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D015153:Blotting, Western; D002002:Brucella / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008954:Models, Biological
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins