title: The relative role of neutrophils and platelets in the local accumulation of circulating lymphocytes at sites of ionophore A23187 inoculation.
abstract: The early cellular infiltrate at inflammatory sites consists predominantly of neutrophils and cells of the monocyte/macrophage lineage. The mechanism by which circulating, unsensitized lymphocytes accumulate at sites of inflammation is unknown. The pattern of accumulation of 111indium-labeled circulating thymocytes in response to local injections of the ionophore A23187 was studied and compared with the pattern of (125)iodinated albumin accumulation as a measure of vascular permeability. The kinetics of thymocyte accumulation differed from those of vascular permeability. Sublethal total-body irradiation (750 rads) markedly decreased thymocyte accumulation but had little effect on vascular permeability. Irradiation of the local site alone had no effect. T lymphocyte, T lymphoblast, and platelet accumulation generally followed the same pattern as thymocytes. Intravenous injection of neutrophils, but not platelets, partially restored lymphocyte accumulation in vivo in irradiated mice via a pathway involving the circulating neutrophil, and seemed to be independent of changes in vascular permeability.
authors.affiliation: University of Pittsburgh, Pennsylvania.
authors.forename: J M
authors.initials: JM
authors.lastname: Hayes
authors.forename: R L
authors.initials: RL
authors.lastname: Simmons
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D000001:Calcimycin / Q000494:pharmacology*; D002199:Capillary Permeability / Q000187:drug effects; D005260:Female; D007205:Indium Radioisotopes; D007249:Inflammation; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000502:physiology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D013997:Time Factors; D014916:Whole-Body Irradiation
chemical_list: D007205:Indium Radioisotopes; D000001:Calcimycin