title: Construction and analysis of infectious transcripts synthesized from full-length cDNA clones of both genomic RNAs of pea early browning virus.
abstract: Full-length cDNA clones of both RNAs of pea early browning virus have been constructed. Synthetic transcripts derived in vitro from these clones are infectious when inoculated onto plants. Electron microscopy revealed the presence of virions in transcript-inoculated plants, and both purified RNA and virions isolated from such plants could be used to infect other plants. Transcripts of RNA1 alone were able to replicate and spread systemically which is a characteristic of members of the tobravirus group of plant viruses.
authors.affiliation: Department of Virus Research, John Innes Institute for Plant Science Research, Norwich, United Kingdom.
authors.forename: S A
authors.initials: SA
authors.lastname: MacFarlane
authors.forename: C V
authors.initials: CV
authors.lastname: Wallis
authors.forename: S C
authors.initials: SC
authors.lastname: Taylor
authors.forename: M G
authors.initials: MG
authors.lastname: Goulden
authors.forename: K R
authors.initials: KR
authors.lastname: Wood
authors.forename: J W
authors.initials: JW
authors.lastname: Davies
mesh_terms: D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005814:Genes, Viral; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D010942:Plant Viruses / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D010947:Plants, Toxic; D016133:Polymerase Chain Reaction; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D014026:Nicotiana / Q000382:microbiology; D014158:Transcription, Genetic; D015678:Viral Structural Proteins / Q000235:genetics; D014779:Virus Replication
chemical_list: D009841:Oligonucleotides; D012367:RNA, Viral; D015678:Viral Structural Proteins; D004247:DNA