Purification of a stage-specific and sequence-specific DNA-binding protein for the arylphorin gene of Sarcophaga peregrina.
A protein that binds specifically to the nucleotide sequence ACCACAACA located at residues -247 to -255 upstream of the +1 site of the arylphorin gene of Sarcophaga peregrina was purified to homogeneity from fat body nuclei of third instar larvae. This DNA-binding protein consisted of two subunits with molecular masses of 40 kDa and 42 kDa, respectively. Accurate transcription initiation of a truncated arylphorin gene in a nuclear extract of NIH-Sape-4 cells, an embryonic cell line of Sarcophaga, was significantly enhanced in the presence of the purified DNA-binding protein.
Kim
J W
JW
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Komano
H
H
Natori
S
S
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology / Q000528:radiation effects; D003850:Deoxyribonuclease I; D004175:Diptera / Q000235:genetics*; D005216:Fat Body / Q000737:chemistry; D005786:Gene Expression Regulation / Q000502:physiology; D006023:Glycoproteins; D007301:Insect Hormones / Q000235:genetics*; D019476:Insect Proteins; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D014158:Transcription, Genetic; D014466:Ultraviolet Rays
D004268:DNA-Binding Proteins; D006023:Glycoproteins; D007301:Insect Hormones; D019476:Insect Proteins; D009838:Oligodeoxyribonucleotides; C041137:storage proteins, Insecta; D003850:Deoxyribonuclease I