title: Analysis of tropane alkaloids with thermospray high-performance liquid chromatography-mass spectrometry.
abstract: A thermospray high-performance liquid chromatography-mass spectrometry method for analysis of hyoscyamine and scopolamine in plant cell culture samples is described. The alkaloids were separated on a polymeric reversed-phase column with an alkaline ammonium acetate buffer-acetonitrile eluent. Selected-ion recording of the protonated molecular ions was used for quantitation of the compounds. The compounds were fragmented by discharge-assisted ionization and elevated thermospray capillary temperatures or ion repeller potentials.
authors.affiliation: Department of Pharmaceutical Chemistry, University of Kuopio, Finland.
authors.forename: S
authors.initials: S
authors.lastname: Auriola
authors.forename: A
authors.initials: A
authors.lastname: Martinsen
authors.forename: K M
authors.initials: KM
authors.lastname: Oksman-Caldentey
authors.forename: T
authors.initials: T
authors.lastname: Naaranlahti
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D010946:Plants, Medicinal / Q000032:analysis; D011786:Quality Control; D012601:Scopolamine / Q000032:analysis; D014326:Tropanes / Q000032:analysis*
chemical_list: D000470:Alkaloids; D014326:Tropanes; C100236:homatropine; D012601:Scopolamine