Regulation of microtubule associated protein 2 (MAP2) expression by nerve growth factor in PC12 cells.
In the presence of nerve growth factor (NGF), PC12 cells cease to divide and differentiate, extending long microtubule-containing neurites. We showed by immunoblot analysis that MAP2 was detectable in PC12 after 4 days of NGF treatment and that its levels increased five- to sevenfold after 12 days of NGF treatment. The apparent molecular weight of MAP2 in PC12 cells was similar to that of rat brain MAP2 (280,000), with a doublet representing the MAP2 isoforms. However, the relative levels of MAP2 in differentiated PC12 cells were 5-10% of those found in rat brain. Immunofluorescence analysis of NGF-treated PC12 cells revealed that MAP2 co-localized with tubulin and was present in cell bodies and neurites. Northern blot analysis showed that the levels of MAP2 mRNA increased in PC12 cells during NGF-treatment in a pattern that paralleled the protein levels, suggesting that MAP2 expression is transcriptionally regulated.
Fischer
I
I
Department of Developmental Neurobiology, E. K. Shriver Center, Waltham, Massachusetts 02254.
Richter-Landsberg
C
C
Safaei
R
R
D000310:Adrenal Gland Neoplasms; D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects*; D015151:Immunoblotting; D007700:Kinetics; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000494:pharmacology*; D010673:Pheochromocytoma; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects
D008869:Microtubule-Associated Proteins; D009414:Nerve Growth Factors; D015347:RNA Probes; D012333:RNA, Messenger