title: Validity of flow cytometry for cross-match evaluation in clinical renal transplantation.
abstract: This paper reports a 2-year experience of more than 5,000 cross-match tests for renal transplantation. Tests were performed by means of both standard light microscopy and an innovatory method based on flow cytometry, an up-to-date investigative technique for computerized analysis of individual cell characteristics. Flow cytometry allowed a better detection of weak positive reactions (false-negative cross-matches) than light microscopy, thus reducing the risk of selecting candidates with donor presensitization. Transplant clinical outcome supported the value of this original and advanced technological method.
authors.affiliation: Institute of Nephrology, University of Bologna, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Stefoni
authors.forename: A
authors.initials: A
authors.lastname: Nanni-Costa
authors.forename: A
authors.initials: A
authors.lastname: Buscaroli
authors.forename: L C
authors.initials: LC
authors.lastname: Borgnino
authors.forename: S
authors.initials: S
authors.lastname: Iannelli
authors.forename: C
authors.initials: C
authors.lastname: Raimondi
authors.forename: M P
authors.initials: MP
authors.lastname: Scolari
authors.forename: G
authors.initials: G
authors.lastname: Feliciangeli
authors.forename: V
authors.initials: V
authors.lastname: Bonomini
mesh_terms: D000906:Antibodies / Q000032:analysis; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry / Q000379:methods*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology
chemical_list: D000906:Antibodies