High-velocity mechanical DNA transfer of the chloramphenicolacetyl transferase gene into rodent liver, kidney and mammary gland cells in organ explants and in vivo.
Mouse and rat liver, kidney and mammary gland explants were bombarded with high-velocity microprojectiles carrying a chloramphenicolacetyl transferase gene under different promoters (pTAT-cat, p chi-Casein-cat, p beta-Casein-cat). The expression of a CAT gene was revealed in all organ explants 24 h after transfection. The most pronounced expression was found when a TAT-CAT construction was used. In experiments in vivo rat liver was bombarded in situ with microprojectiles carrying pTAT-cat DNA. A marked activity of the CAT gene was detected 24 h after the bombardment.
Zelenin
A V
AV
Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
Alimov
A A
AA
Titomirov
A V
AV
Kazansky
A V
AV
Gorodetsky
S I
SI
Kolesnikov
V A
VA
D000818:Animals; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics*; D004247:DNA / Q000737:chemistry; D066298:In Vitro Techniques; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008321:Mammary Glands, Animal / Q000201:enzymology*; D011401:Promoter Regions, Genetic; D051381:Rats; D011919:Rats, Inbred Strains; D014162:Transfection; D014170:Transformation, Genetic
D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase