Effect of chronic alcohol consumption on rat uterine spontaneous motility, prostaglandin production and triglyceride metabolism.
The spontaneous isometric developed tension (IDT), the synthesis and release of prostaglandins (PGs) into the incubating medium and the metabolism of triglycerides (TGs) in uterine strips isolated from controls and chronic ethanol fed rats, were studied. In order to observe how the uterus of rats fed alcohol reacts during a situation of metabolic emergency, the above mentioned studies were done in the presence or in the absence of glucose in the incubating medium. The decrement of IDT as time progressed was significantly greater in strips obtained from rats which had been drinking 20% ETOH than in controls. Nevertheless, the absolute magnitude of the initial IDT was similar in both groups. On the other hand, the decline of the frequency of contractions (FC) of uterine strips isolated from controls and from ETOH-exposed rats, after 60 min of spontaneous activity was similar. When the uterine strips isolated from ETOH-exposed and from control rats were suspended in glucose-free solution they exhibited the same decrement of IDT and FC after 60 min of activity. The basal release of PGE1 and PGE2 was similar in control tissues incubated in medium containing glucose, but the output of PGE2 was significantly smaller than that of PGE1 in uterine strips isolated from ETOH-exposed rats. The production of PGE1 and PGE2 by uteri suspended in glucose-free medium was similar in control preparations. On the contrary the release of both PGs differs in uterine strips from ETOH-exposed rats, i.e. the output of PGE2 was significantly smaller than in controls and the release of PGE1 increased around 4-fold in comparison with controls.(ABSTRACT TRUNCATED AT 250 WORDS)
Chaud
M A
MA
Centro de Estudios Farmácológicos y Botánicos (CEFYBO), Universidad de Buenos Aires, Argentina.
Gonzalez
E T
ET
Franchi
A M
AM
Fernandez Pardal
J
J
Gimeno
A L
AL
Gimeno
M A
MA
D000437:Alcoholism / Q000378:metabolism / Q000503:physiopathology*; D000527:Alprostadil / Q000096:biosynthesis / Q000378:metabolism; D000818:Animals; D015232:Dinoprostone / Q000096:biosynthesis / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D011453:Prostaglandins / Q000096:biosynthesis* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000378:metabolism*; D014590:Uterine Contraction; D014599:Uterus / Q000378:metabolism / Q000503:physiopathology*
D011453:Prostaglandins; D014280:Triglycerides; D000527:Alprostadil; D015232:Dinoprostone