title: Comparison of the Gen-Probe PACE 2 system, direct fluorescent-antibody, and cell culture for detecting Chlamydia trachomatis in cervical specimens.
abstract: A chemiluminescent-labeled DNA probe (Gen-Probe PACE 2 System) for detection of Chlamydia trachomatis in endocervical specimens was evaluated. For each specimen, tissue cell culture, direct immunofluorescent staining (DFA), and DNA probe assay were performed. Thirty of 318 (9.4%) specimens were positive for C. trachomatis. The sensitivities, specificities, and positive and negative predictive values of the DNA probe compared with cell culture were 93%, 98%, 85%, and 99%, respectively, and for DFA these same values were 81%, 99%, 83%, and 99%, respectively. The Gen-Probe PACE 2 System is a reliable method for the rapid detection of endocervical chlamydial infection.
authors.affiliation: Department of Pathology, University of Nebraska Medical Center, Omaha 68198-6495.
authors.forename: P C
authors.initials: PC
authors.lastname: Iwen
authors.forename: T M
authors.initials: TM
authors.lastname: Blair
authors.forename: G L
authors.initials: GL
authors.lastname: Woods
mesh_terms: D002478:Cells, Cultured; D002584:Cervix Uteri / Q000737:chemistry / Q000166:cytology* / Q000382:microbiology; D002690:Chlamydia Infections / Q000175:diagnosis / Q000473:pathology; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification*; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008163:Luminescent Measurements; D011237:Predictive Value of Tests
chemical_list: D015342:DNA Probes; D004279:DNA, Viral