Developmental disturbance of rat cerebral cortex following prenatal low-dose gamma-irradiation: a quantitative study.
Pregnant rats were exposed to a single whole-body gamma-irradiation on Day 15 of gestation at a dose of 0.27, 0.48, 1.00, or 1.46 Gy. They were allowed to give birth and the offspring were killed at 6 or 12 weeks of age for microscopic and electron microscopic examinations of the cerebrum. Their body weight, brain weight, cortical thickness, and numerical densities of whole cells and synapses in somatosensory cortex were examined. Growth of the dendritic arborization of layer V pyramidal cells was also examined quantitatively with Golgi-Cox specimens. A significant dose-related reduction in brain weight was found in all irradiated groups. Neither gross malformation nor abnormality of cortical architecture was observed in the groups exposed to 0.27 Gy. A significant change was found in thickness of cortex in the groups exposed to 0.48 Gy or more. Cell packing density increased significantly in the group exposed to 1.00 Gy. Significant reduction in the number of intersections of dendrites with the zonal boundaries were found in the groups exposed to 0.27 Gy or more. There was no difference in the numerical density of synapses in layer I between the control and irradiated groups. These results suggested that doses as low as 0.27 Gy could cause a morphologically discernible change in the mammalian cerebrum.
Fukui
Y
Y
Department of Anatomy, Kagawa Medical School, Japan.
Hoshino
K
K
Hayasaka
I
I
Inouye
M
M
Kameyama
Y
Y
D000818:Animals; D002540:Cerebral Cortex / Q000254:growth & development / Q000473:pathology / Q000528:radiation effects*; D005260:Female; D005720:Gamma Rays; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D013003:Somatosensory Cortex / Q000254:growth & development / Q000473:pathology / Q000528:radiation effects