title: Amylin activates glycogen phosphorylase in the isolated soleus muscle of the rat.
abstract: The pancreatic beta-cell hormone amylin acts in isolated rat skeletal muscle to decrease insulin-stimulated incorporation of glucose into glycogen. It also increases blood levels of lactate and glucose in fasted rats in vivo. However, it remained uncertain whether amylin exerts direct effects to stimulate muscle glycogenolysis. We now report that amylin caused a dose-dependent increase in activity of muscle glycogen phosphorylase in isolated rat soleus muscle by stimulating phosphorylase a. Insulin inhibited amylin-stimulated activation of phosphorylase. Effects of amylin to stimulate muscle glycogenolysis are consistent with observed effects of amylin in vivo and could be a major mechanism whereby amylin modulates carbohydrate metabolism.
authors.affiliation: Amylin Corporation, San Diego, CA 92121.
authors.forename: A A
authors.initials: AA
authors.lastname: Young
authors.forename: D M
authors.initials: DM
authors.lastname: Mott
authors.forename: K
authors.initials: K
authors.lastname: Stone
authors.forename: G J
authors.initials: GJ
authors.lastname: Cooper
mesh_terms: D000682:Amyloid / Q000494:pharmacology*; D000818:Animals; D004789:Enzyme Activation; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D058228:Islet Amyloid Polypeptide; D007700:Kinetics; D009132:Muscles / Q000201:enzymology*; D010762:Phosphorylase a / Q000378:metabolism*; D006005:Phosphorylases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000682:Amyloid; D007328:Insulin; D058228:Islet Amyloid Polypeptide; D010762:Phosphorylase a; D006005:Phosphorylases