title: Light-harvesting complexes of brown algae. Biochemical characterization and immunological relationships.
abstract: The pigment composition of the light-harvesting complexes isolated from several brown algae belonging to different orders has been analysed by reverse-phase HPLC. Relative to whole chloroplasts, they were markedly enriched in Chl c, fucoxanthin and violaxanthin and conversely depleted in Chl a. The relative molar proportions of the 4 main pigments (Chl a/Chl c/fucoxanthin/violaxanthin) ranged from 100:18:76:6 to 100:30:107:17. The protein moiety of LH complexes of all the species studied were composed of one or two main polypeptide components in the range of 19-22 kDa. These polypeptide subunits were arranged in polymeric particles about 240 kDa in Laminaria saccharina. A polyclonal antibody raised against the LH polypeptide of Fucus serratus has been tested on LH apoproteins of other Chromophytes and Chlorophytes. Phylogenic implications of these results are discussed.
authors.affiliation: Laboratoire des Biomembranes et Surfaces Cellulaires Végétales, UA CNRS DO311, Ecole Normale Supérieure, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Passaquet
authors.forename: J C
authors.initials: JC
authors.lastname: Thomas
authors.forename: L
authors.initials: L
authors.lastname: Caron
authors.forename: N
authors.initials: N
authors.lastname: Hauswirth
authors.forename: F
authors.initials: F
authors.lastname: Puel
authors.forename: C
authors.initials: C
authors.lastname: Berkaloff
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000908:Antibodies, Fungal / Q000276:immunology; D002338:Carotenoids / Q000031:analogs & derivatives / Q000032:analysis / Q000276:immunology; D002736:Chloroplasts / Q000737:chemistry; D003429:Cross Reactions; D000459:Phaeophyceae / Q000032:analysis*; D010860:Pigments, Biological / Q000032:analysis* / Q000276:immunology; D013050:Spectrometry, Fluorescence; D014461:Ultracentrifugation; D024341:Xanthophylls; D019207:beta Carotene / Q000031:analogs & derivatives*
chemical_list: D000596:Amino Acids; D000908:Antibodies, Fungal; D010860:Pigments, Biological; D024341:Xanthophylls; D019207:beta Carotene; C025164:fucoxanthin; D002338:Carotenoids; C005613:violaxanthin