title: Interactions of nucleic acids with distamycins. Binding of Dst-3 to d(CGTTTAAACG)2 and d(CGTACGTACG)2.
abstract: The binding between Distamycin 3 and the palindromic duplexes d(CGTTTAAACG)2 and d(CGTACGTACG)2 was investigated by two independent techniques: UV-Vis absorption in the Job's plot approach and Induced Circular Dichroism. Both decamers bind two molecules of peptide per duplex, with close overall affinities. This result indicates that a row of six A:T base pairs can accommodate two molecules of drug and that the minimal binding site of Distamycin 3 can consist of just two A:T base pairs.
authors.affiliation: I.Co.C.E.A.-CNR, Bologna, Italy.
authors.forename: M L
authors.initials: ML
authors.lastname: Capobianco
authors.forename: F P
authors.initials: FP
authors.lastname: Colonna
authors.forename: A
authors.initials: A
authors.lastname: Forni
authors.forename: A
authors.initials: A
authors.lastname: Garbesi
authors.forename: S
authors.initials: S
authors.lastname: Iotti
authors.forename: I
authors.initials: I
authors.lastname: Moretti
authors.forename: B
authors.initials: B
authors.lastname: Samori
authors.forename: L
authors.initials: L
authors.lastname: Tondelli
mesh_terms: D001483:Base Sequence; D002942:Circular Dichroism; D004214:Distamycins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004214:Distamycins; D009692:Nucleic Acid Heteroduplexes; D009838:Oligodeoxyribonucleotides