Bovine corneal protein 54K (BCP54) is a homologue of the tumor-associated (class 3) rat aldehyde dehydrogenase (RATALD).
Amino acid (aa) sequence data from Staphylococcus areas V8 protease-digested bovine corneal 54-kDa protein (BCP54) fragments were utilized to derive mixed oligodeoxyribonucleotide (oligo) primers complementary to the reverse translation products of these sequences. These degenerate oligo primers were used to prime the amplification of BCP54 sequence from bovine corneal epithelial cell cDNA. The cDNA probe generated by this mixed oligo-primed amplification of cDNA was cloned and dideoxy-sequenced. A search of the GenBank database (version 63.0) revealed extensive sequence similarity to the cDNA encoding tumor-associated rat liver (class 3) aldehyde dehydrogenase (RATALD). Nucleotide (nt) and aa sequence alignment of the BCP54 translation product reveals it is 78% and 84% homologous with RATALD at the nt and aa levels, respectively. Conservation of aa sequence elements common to the aldehyde dehydrogenase family thought to be of structural/functional significance is further substantiated by this analysis. Included in the discussion is the likelihood that gene sharing (genes encoding metabolic enzymes and other stable proteins) may extend to the cornea.
Cooper
D L
DL
Department of Pathology, Duke University, Durham, NC 27710.
Baptist
E W
EW
Enghild
J J
JJ
Isola
N R
NR
Klintworth
G K
GK
D000444:Aldehyde Dehydrogenase / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D015342:DNA Probes; D005136:Eye Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D012697:Serine Endopeptidases
D015342:DNA Probes; D005136:Eye Proteins; D010446:Peptide Fragments; C030863:corneal protein 54, bovine; D000444:Aldehyde Dehydrogenase; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase