Improved cloning efficiency of polymerase chain reaction (PCR) products after proteinase K digestion.
Crowe
J S
JS
Department of Cell Biology, Wellcome Research Laboratories, Bechenham, Kent, UK.
Cooper
H J
HJ
Smith
M A
MA
Sims
M J
MJ
Parker
D
D
Gewert
D
D
D003001:Cloning, Molecular / Q000379:methods*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D019286:Endopeptidase K; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012697:Serine Endopeptidases / Q000378:metabolism*; D019914:Taq Polymerase; D013698:Templates, Genetic
C014609:IgK; D007136:Immunoglobulins; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase; D012697:Serine Endopeptidases; D019286:Endopeptidase K