title: Comparison of thrombin-antithrombin complex (TAT) levels and fibrinopeptide A following thrombin incubation of human plasma using hirudin as an inhibitor of TAT formation.
abstract: Following incubation of citrated plasma with human thrombin, the interaction of thrombin with antithrombin III was measured as thrombin-antithrombin complex (TAT) concentration. Comparison was made to thrombin activity on fibrinogen, assayed as fibrinopeptide A (FPA). Light scattering studies were included to evaluate polymerization of fibrin. Hirudin, at a final concentration of 100 U/ml, effectively inhibited TAT generation at final thrombin concentrations below 0.4 NIH U/ml. Hirudin by itself did not affect the TAT ELISA analysis. TAT and FPA values correlated closely (r = 0.83, p less than 0.001) and may equally well represent in vitro thrombin activity.
authors.affiliation: Haematological Research Laboratory, University Clinic, Ullevål Hospital, Oslo, Norway.
authors.forename: A K
authors.initials: AK
authors.lastname: Haaland
authors.forename: O H
authors.initials: OH
authors.lastname: Skjønsberg
authors.forename: K
authors.initials: K
authors.lastname: Gravem
authors.forename: R
authors.initials: R
authors.lastname: Ruyter
authors.forename: H C
authors.initials: HC
authors.lastname: Godal
mesh_terms: D000990:Antithrombin III / Q000032:analysis* / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005344:Fibrinopeptide A / Q000032:analysis*; D006629:Hirudins / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008027:Light; D008297:Male; D010447:Peptide Hydrolases / Q000032:analysis*; D012542:Scattering, Radiation; D013917:Thrombin / Q000032:analysis / Q000037:antagonists & inhibitors*
chemical_list: D006629:Hirudins; C046193:antithrombin III-protease complex; D005344:Fibrinopeptide A; D000990:Antithrombin III; D010447:Peptide Hydrolases; D013917:Thrombin