title: Coralline shape of the bacterial nucleoid after cryofixation.
abstract: A new procedure of immunostaining sections of cryofixed and freeze-substituted Escherichia coli shows that DNA extends from its bulk into small ribosome-free spaces throughout the cytoplasm, resulting in a coralline-shaped nucleoid. Low-resolution imaging of a bacterium reconstructed from serial sections demonstrated that the small excrescencies are not resolved. The resulting photograph shows the same features as phase-contrast light micrographs.
authors.affiliation: Department of Microbiology, University of Basel, Switzerland.
authors.forename: B
authors.initials: B
authors.lastname: Bohrmann
authors.forename: W
authors.initials: W
authors.lastname: Villiger
authors.forename: R
authors.initials: R
authors.lastname: Johansen
authors.forename: E
authors.initials: E
authors.lastname: Kellenberger
mesh_terms: D002843:Chromatin / Q000648:ultrastructure; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000648:ultrastructure*; D005404:Fixatives; D007150:Immunohistochemistry; D007158:Immunologic Techniques; D008854:Microscopy, Electron
chemical_list: D002843:Chromatin; D004269:DNA, Bacterial; D005404:Fixatives