Detection of human T-cell leukemia virus type I proviral sequences from fixed tissues of seropositive patients.
Fixed lymphoma tissues from 11 patients seropositive for human T-cell leukemia virus type I (HTLV-I) antibodies were analyzed for proviral sequences by the polymerase chain reaction. Typical adult T-cell leukemia/lymphomas (ATLLs) from nine patients were positive for HTLV-I sequences. In contrast, one of two lymphomas with immunophenotypes atypical for ATLL was negative for HTLV-I. This HTLV-I DNA-negative lymphoma, although present in an HTLV-I-seropositive patient, was therefore reclassified. The HTLV-I tax gene was always detected in the ATLL tissues, whereas segments of the pol gene were not detected in half the cases. These studies demonstrate that fixed non-ATLL specimens can be distinguished from ATLL specimens.
Shibata
D
D
Department of Pathology, University of Southern California School of Medicine, Los Angeles.
Tokunaga
M
M
Sasaki
N
N
Nanba
K
K
D000328:Adult; D000368:Aged; D004279:DNA, Viral / Q000235:genetics*; D003937:Diagnosis, Differential; D005260:Female; D016355:Genes, pX / Q000235:genetics; D015751:Genes, pol / Q000235:genetics; D006679:HIV Seropositivity / Q000235:genetics / Q000382:microbiology*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D016130:Immunophenotyping; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000175:diagnosis / Q000235:genetics / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics* / Q000302:isolation & purification; D012698:Serologic Tests
D004279:DNA, Viral