Identification of a cis-acting positive regulatory element of the glial fibrillary acidic protein gene.
Developmental regulation of astrocyte-specific expression of the glial fibrillary acidic protein (GFAP) gene reflects transition of immature glioblasts to mature astrocytes. Described here is the cloning and sequencing of the 5'-flanking region of the mouse GFAP gene. It contains a glial-specific positive cis-acting regulatory element that directs preferential expression of a linked reporter gene when transfected into GFAP-positive glioblastoma cells. Sequence analysis of this region revealed the presence of a putative AP-1 binding site, implying a possible role for AP-1 factors in the astroglial-specific expression of the GFAP gene.
Sarid
J
J
Department of Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115.
D001253:Astrocytes / Q000378:metabolism; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000235:genetics; D005796:Genes; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics*; D005910:Glioma / Q000378:metabolism; D008969:Molecular Sequence Data; D013237:Stereoisomerism; D014407:Tumor Cells, Cultured
D005904:Glial Fibrillary Acidic Protein; D004247:DNA