title: Assessment of HIV status using the polymerase chain reaction in antibody-positive patients and high-risk antibody-negative haemophiliacs.
abstract: The polymerase chain reaction (PCR) was used to identify the presence of DNA sequences homologous to HIV-1 in the buffy-coat leukocytes of antibody-positive and antibody-negative individuals in a haemophiliac population. The presence of HIV sequences was demonstrated in all of the antibody-positive haemophiliacs with the exception of one patient who was repeatedly negative. None of the seronegative haemophiliacs gave an overall positive result, although there were clear differences between this population and the negative controls who were examined. We conclude that, in our hands, PCR represents a reliable test which represents a useful diagnostic advance in HIV medicine.
authors.affiliation: Department of Clinical Immunology, Royal Prince Alfred Hospital, Sydney, Australia.
authors.forename: G I
authors.initials: GI
authors.lastname: Shoebridge
authors.forename: L
authors.initials: L
authors.lastname: Barone
authors.forename: A
authors.initials: A
authors.lastname: Wing-Simpson
authors.forename: B H
authors.initials: BH
authors.lastname: Bennetts
authors.forename: B N
authors.initials: BN
authors.lastname: Nightingale
authors.forename: W J
authors.initials: WJ
authors.lastname: Hensley
authors.forename: P A
authors.initials: PA
authors.lastname: Gatenby
mesh_terms: D001483:Base Sequence; D004279:DNA, Viral / Q000097:blood*; D005069:Evaluation Studies as Topic; D006678:HIV / Q000235:genetics / Q000302:isolation & purification; D015658:HIV Infections / Q000175:diagnosis* / Q000382:microbiology; D006679:HIV Seropositivity / Q000097:blood* / Q000382:microbiology; D006467:Hemophilia A / Q000097:blood* / Q000382:microbiology; D008214:Lymphocytes / Q000382:microbiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000302:isolation & purification; D012307:Risk Factors
chemical_list: D004279:DNA, Viral