Pheromone-dependent phosphorylation of the yeast STE12 protein correlates with transcriptional activation.
Haploid a and alpha cells of yeast respond to the pheromones alpha- and a-factor, respectively, by increasing the transcription of many genes whose products are essential for mating. The STE12 protein acts in this process by binding to the DNA sequence that mediates the increased transcription of pheromone-responsive genes. We show here that a hybrid protein containing STE12 fused to the DNA-binding domain of GAL4 can activate transcription of a reporter gene containing GAL4-binding sites but only after treatment of cells with pheromone. Thus, STE12 alone, when bound to DNA, is sufficient to mediate pheromone-induced transcription. By constructing hybrids of different STE12 regions with the GAL4 domain, we map the domain of STE12 necessary for this activation to the central third of the protein. Upon alpha-factor treatment, the hybrid of GAL4 with the complete STE12 sequence is rapidly phosphorylated, with kinetics consistent with the observed transcriptional induction of pheromone-responsive genes. The domain of STE12 necessary for this phosphorylation correlates with that involved in transcriptional activation. We propose that induction of pheromone-responsive genes is mediated by phosphorylation of STE12 to alter its activation function but not its DNA-binding ability.
Song
D
D
Department of Microbiology, State University of New York, Stony Brook 11794.
Dolan
J W
JW
Yuan
Y L
YL
Fields
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S
D001665:Binding Sites; D003001:Cloning, Molecular; D003513:Cycloheximide; D005656:Fungal Proteins / Q000378:metabolism*; D007700:Kinetics; D010675:Pheromones / Q000502:physiology*; D010766:Phosphorylation; D010957:Plasmids; D011233:Precipitin Tests; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D015003:Yeasts / Q000235:genetics*
D005656:Fungal Proteins; D010675:Pheromones; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D003513:Cycloheximide