Visualization of hypoxia-induced glutamate release in gerbil hippocampal slice.
Microfluorometry was used to investigate distribution of hypoxia-induced release of glutamate. Mongolian gerbil hippocampal slice was perfused in a medium containing glutamate dehydrogenase and NAD+. Release of glutamate into extracellular space caused an increase in fluorescence due to the formation of NADH. The hypoxia-induced release of glutamate was gradually increased throughout the slice: no significant difference was detected among CA1 region, CA3 region and the dentate gyrus.
Mitani
A
A
Department of Physiology, School of Medicine, Ehime University, Japan.
Kadoya
F
F
Nakamura
Y
Y
Kataoka
K
K
D000818:Animals; D005849:Gerbillinae; D005969:Glutamate Dehydrogenase; D005971:Glutamates / Q000032:analysis / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology / Q000502:physiology*; D000860:Hypoxia; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D009243:NAD / Q000032:analysis
D005971:Glutamates; D009243:NAD; D005969:Glutamate Dehydrogenase