[A simple method for estimation of phospholipids in biological objects].
A simple method to estimate phospholipids is elaborated. This method is based on determination of optical density of phospholipid-molybdate complexes in chloroform. The method is used for the quantitative determination of phospholipids in biomembranes, liposomes and blood serum without their preliminary extraction, as well as in chloroform, methanol and chloroform-methanol solutions. It is also modified for the phospholipids determination in chromatographic fractions on silufol plates.
Lemeshko
V V
VV
Brovkovich
V M
VM
Listopad
A P
AP
D000818:Animals; D002725:Chloroform; D008081:Liposomes / Q000032:analysis; D008563:Membrane Lipids / Q000032:analysis; D000432:Methanol; D010743:Phospholipids / Q000032:analysis* / Q000097:blood; D051381:Rats; D013053:Spectrophotometry
D008081:Liposomes; D008563:Membrane Lipids; D010743:Phospholipids; D002725:Chloroform; D000432:Methanol