Evaluation of an indirect enzyme-linked immunosorbent assay for screening antibody against aflatoxins.
Enzyme-linked immunosorbent assay screening of antibody produced against aflatoxin was accomplished by a new and simple procedure. To demonstrate the new indirect ELISA technique used, antibody against aflatoxin M1 was produced in female BALB/CJ mice by immunization with an aflatoxin M1-bovine serum albumin conjugate. Instead of coating test-plate wells with purified antibody (direct ELISA) or synthesizing a second protein-aflatoxin conjugate (aflatoxin M1-poly-L-lysine) to coat test-plate wells, wells were coated with the readily available aflatoxin M1-bovine serum albumin and aflatoxin B1-bovine serum albumin. This method, applicable for any aflatoxin conjugated by the common cyclopentano-carboxymethoxyl-oxime technique, eliminates the more time-consuming and technically difficult portions of earlier direct and indirect ELISA. The new technique can be valuable in continued efforts toward development of new and improved immunoassays against aflatoxin metabolites.
Holladay
S D
SD
Department of Anatomy, Physiological Sciences and Radiology, College of Veterinary Medicine, North Carolina State University, NC 27606.
Brownie
C F
CF
Corbett
W T
WT
Talley
D D
DD
D000348:Aflatoxins / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D051379:Mice
D000348:Aflatoxins; D000906:Antibodies