title: Chromatographic developments in low flow delivery for liquid chromatography-mass spectrometry.
abstract: A method has been demonstrated for producing accurate, reproducible gradients at low microliter per minute flow-rates suitable for liquid chromatographic-mass spectrometric (LC-MS) applications using capillary chromatography columns. The technique employs a low-cost, well characterized balance-column flow-splitter which can be simply added to a high-performance LC-MS solvent delivery system. Performance of the pump for LC-MS techniques at higher flow-rates is preserved.
authors.affiliation: Waters Chromatography Division, Millipore Corporation, Milford, MA 01757.
authors.forename: M P
authors.initials: MP
authors.lastname: Balogh
authors.forename: C C
authors.initials: CC
authors.lastname: Stacey
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000295:instrumentation*; D006868:Hydrolysis; D013058:Mass Spectrometry / Q000295:instrumentation*; D010446:Peptide Fragments / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D014357:Trypsin; D014783:Viscosity
chemical_list: D010446:Peptide Fragments; D014357:Trypsin