A method for testing the stability of a steady-state system during the calculation of a response to large changes in regulator concentrations.
A modification of the 'finite decomposition' method (Crabtree and Newsholme (1985) Curr. Top. Cell. Regul. 25, 21-76) for calculating physiological responses from sensitivities is described, to enable the system to be tested for stability at each step of the procedure. Instability is indicated by a change of sign of the determinant of the square matrix (N) in the governing equation for the system. The method cannot be used to predict a change of sign of the determinant of the square matrix (N) in the governing equation for the system. The method cannot be used to predict responses beyond any step at which instability occurs.
Crabtree
B
B
Rowett Research Institute, Aberdeen, Scotland, UK.
Newsholme
E A
EA
D000494:Allosteric Regulation; D055598:Chemical Phenomena; D002621:Chemistry; D004798:Enzymes / Q000378:metabolism; D008433:Mathematics; D008660:Metabolism; D012680:Sensitivity and Specificity
D004798:Enzymes