Decreased glutathione S-transferase activity in mice livers by acute treatment with lead, independent of alteration in glutathione content.
Glutathione S-transferase (GST) activity and glutathione content in livers of male mice were assayed after acute administration of lead acetate (100 mg/kg i.p.). Fall in GST activity of lead-treated mice followed the decrease in glutathione content with a delay of more than 1 day. In addition, L-methionine (250 mg/kg i.p.) pretreatment did not antagonize the fall in GST activity induced by lead. In contrast, diethyl maleate, a potent glutathione depletor, increased GST activity. Thus, lead administration reduced the ability of the phase II reaction of drug metabolism, although glutathione depletion was not necessarily a critical factor for impairmen of GST per se.
Nakagawa
K
K
Department of Food Science, Kyoto Women's University, Japan.
D000818:Animals; D003545:Cysteine / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D007854:Lead / Q000008:administration & dosage / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D008298:Maleates / Q000633:toxicity; D008715:Methionine / Q000494:pharmacology; D051379:Mice; D010084:Oxidation-Reduction; D013997:Time Factors
D008298:Maleates; D007854:Lead; D008715:Methionine; D005982:Glutathione Transferase; C014476:diethyl maleate; D005978:Glutathione; D003545:Cysteine