Expression of the rat aldolase B gene: a liver-specific proximal promoter and an intronic activator.
The nature and location of the cis-acting DNA sequences regulating expression of the rat aldolase B gene has been investigated. Two liver-specific DNAse I hypersensitive sites were detected, one located just upstream from the cap site, the second in the middle of the first, 4.8-kbp-long, intron. A fragment of 190 bp 5' to the cap site behaved as a tissue-specific but weak core promoter: it directed a detectable reporter gene expression in the Hep G2 cells and hepatocytes, but not in fibroblasts. The tissue-specific expression was stimulated at least 16 fold when constructs contained the entire first intron. The intronic activating sequences could be ascribed to an inner 2 kbp fragment in which the downstream liver-specific DNAse I hypersensitive site was located.
Gregori
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ICGM, INSERM U. 129 (Laboratoire de Recherches en Génétique et Pathologie Moléculaires), CHU Cochin, Paris, France.
Ginot
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F
Decaux
J F
JF
Weber
A
A
Berbar
T
T
Kahn
A
A
Pichard
A L
AL
D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics; D016384:Consensus Sequence; D003850:Deoxyribonuclease I; D005347:Fibroblasts / Q000201:enzymology; D005634:Fructose-Bisphosphate Aldolase / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D007438:Introns; D008099:Liver / Q000201:enzymology*; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D012680:Sensitivity and Specificity; D014162:Transfection; D014407:Tumor Cells, Cultured
D015500:Chloramphenicol O-Acetyltransferase; D003850:Deoxyribonuclease I; D005634:Fructose-Bisphosphate Aldolase