Interactions of nucleic acids with distamycins. Binding of Dst-3 to d(CGTTTAAACG)2 and d(CGTACGTACG)2.
The binding between Distamycin 3 and the palindromic duplexes d(CGTTTAAACG)2 and d(CGTACGTACG)2 was investigated by two independent techniques: UV-Vis absorption in the Job's plot approach and Induced Circular Dichroism. Both decamers bind two molecules of peptide per duplex, with close overall affinities. This result indicates that a row of six A:T base pairs can accommodate two molecules of drug and that the minimal binding site of Distamycin 3 can consist of just two A:T base pairs.
Capobianco
M L
ML
I.Co.C.E.A.-CNR, Bologna, Italy.
Colonna
F P
FP
Forni
A
A
Garbesi
A
A
Iotti
S
S
Moretti
I
I
Samori
B
B
Tondelli
L
L
D001483:Base Sequence; D002942:Circular Dichroism; D004214:Distamycins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D004214:Distamycins; D009692:Nucleic Acid Heteroduplexes; D009838:Oligodeoxyribonucleotides