title: Dynamics of human sperm acrosome reaction: relation with in vitro fertilization.
abstract: OBJECTIVE
  Acrosomal status has been studied on human sperm prepared for in vitro fertilization (IVF) and related to the rate of fertilization.
  
  
  METHODS
  A group of 41 men with normal classical semen parameters, included in the IVF program of University of Nice for feminine tubal obstruction (n = 37) or unexplained infertility (n = 4), were evaluated in a prospective study and compared with a control group of 10 fertile donors.
  
  
  METHODS
  Evaluation of acrosome status (spontaneous and A23187-induced acrosome loss) after 6 hours incubation in Ménézo's B2 medium was made by flow cytometry on suspended cells with a new immunofluorescence test recently reported by the authors based on a monoclonal antibody GB24.
  
  
  RESULTS
  Spontaneous acrosome loss remained low even after 6 hours capacitation (mean + 1 SD, 6.5% + 4.9%). Response to A23187 increased with the duration of preincubation with a marked response after 6 hours (29.5% + 8.9%). Low spontaneous acrosome loss (less than mean + 1 SD) and high response to A23187 (greater than mean - 1 SD) were observed in 25 out of 26 cases of group A with a high fertilization rate (greater than 50% fertilized oocytes). A high level of spontaneous acrosome loss and/or a lack of response to A23187 was observed in 2 of 7 cases of group B (fertilization rate less than 50%) and 6 of 8 cases of group C (unexplained unsuccessful fertilization).
  
  
  CONCLUSIONS
  Impaired acrosomal status can be associated with unexplained unsuccessful fertilization.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale (INSERM U210), Faculté de Médecine de Nice, France.
authors.forename: P
authors.initials: P
authors.lastname: Fénichel
authors.forename: M
authors.initials: M
authors.lastname: Donzeau
authors.forename: D
authors.initials: D
authors.lastname: Farahifar
authors.forename: B
authors.initials: B
authors.lastname: Basteris
authors.forename: N
authors.initials: N
authors.lastname: Ayraud
authors.forename: B L
authors.initials: BL
authors.lastname: Hsi
mesh_terms: D000177:Acrosome / Q000187:drug effects / Q000502:physiology*; D000001:Calcimycin / Q000494:pharmacology; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D011446:Prospective Studies; D013075:Sperm Capacitation; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D000001:Calcimycin