A rapid purification method for neurogranin, a brain specific calmodulin-binding protein kinase C substrate.
A rapid purification method is reported for bovine brain neurogranin, a calmodulin-binding protein kinase C (PKC) substrate. This method takes advantage of the fact that the protein remains soluble in 2.5% perchloric acid (PCA) and that it binds to a calmodulin-Sepharose column in the absence of calcium: Other PKC substrate proteins that remain to be identified were also found to share these two properties, suggesting that a class of calmodulin-binding PKC substrates may exist in the brain.
Deloulme
J C
JC
Laboratoire de Neurobiologie Ontogénique, Centre de Neurochimie du CNRS, Strasbourg, France.
Sensenbrenner
M
M
Baudier
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002147:Calmodulin / Q000378:metabolism; D002148:Calmodulin-Binding Proteins; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D051579:Neurogranin; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism; D012995:Solubility
D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; D009419:Nerve Tissue Proteins; D051579:Neurogranin; D011493:Protein Kinase C