Conformation changes of cuttlefish (Euprymna morsei) rhodopsin following photoconversion.
Cuttlefish (Euprymna morsei) rhodopsin solubilized in lauryl ester of sucrose and its photoproduct, acid metarhodopsin, were examined by small-angle X-ray scattering and chromatofocusing to investigate the conformation changes of visual pigment following photoconversion. From spectroscopic studies, it was found that more than 93% of Euprymna rhodopsin could be converted to meta form under the condition of red light irradiation at neutral pH. Since almost pure acid metarhodopsin solution was prepared without changing the specimen concentration, the small-angle X-ray scattering intensities of both pigment-detergent complexes were directly compared. The radius of gyration increased on going from rhodopsin to acid metarhodopsin by approximately 1.5%. There were also discernible changes in the secondary peak intensities. The distribution function, derived by the Fourier transformation of intensity data, showed a significant change around 55 A. The maximum linear dimension of the rhodopsin-detergent complex was about 95 A and hardly changed after illumination. Intensity at zero angle did not change after illumination, suggesting that the aggregation did not occur. The change of the intensity profile could be due to the conformational change of the pigment-detergent monomers. The pI value of rhodopsin determined by chromatofocusing was 5.32 and that of acid metarhodopsin was 5.06, indicating that a few carboxyl groups are newly dissociated. The shift of the protein mass and the charge redistribution were observed following photoconversion.
Hiraki
K
K
Department of Biology, Faculty of Science, Osaka University, Japan.
Hamanaka
T
T
Seidou
M
M
Kito
Y
Y
D000818:Animals; D002942:Circular Dichroism; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D008974:Mollusca; D010777:Photochemistry; D011487:Protein Conformation; D012243:Rhodopsin / Q000737:chemistry*; D012542:Scattering, Radiation; D014965:X-Rays
D012243:Rhodopsin