title: Heterogeneity in human melanoma cell adhesion to cytokine activated endothelial cells correlates with VLA-4 expression.
abstract: Tumor cell attachment to endothelial cells (EC) is one of the critical steps of the metastatic process. It was previously reported that interleukin 1 treatment of EC induces expression of membrane molecules that promote tumor cell adhesion. In this paper we report that a panel of six clones isolated from a human metastatic melanoma presented a marked heterogeneity in their ability to adhere to interleukin 1 activated EC. This was correlated with integrin VLA-4 expression by the clones. Antibodies directed to VLA-4 and to its endothelial ligand INCAM110/VCAM-1 abolished interleukin 1 induced increase in melanoma cell adhesion to EC. These data demonstrate intratumor heterogeneity in the expression of VLA-4 and that this can represent a crucial determinant of tumor cell interaction with EC during secondary spread.
authors.affiliation: Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
authors.forename: I
authors.initials: I
authors.lastname: Martìn-Padura
authors.forename: R
authors.initials: R
authors.lastname: Mortarini
authors.forename: D
authors.initials: D
authors.lastname: Lauri
authors.forename: S
authors.initials: S
authors.lastname: Bernasconi
authors.forename: F
authors.initials: F
authors.lastname: Sanchez-Madrid
authors.forename: G
authors.initials: G
authors.lastname: Parmiani
authors.forename: A
authors.initials: A
authors.lastname: Mantovani
authors.forename: A
authors.initials: A
authors.lastname: Anichini
authors.forename: E
authors.initials: E
authors.lastname: Dejana
mesh_terms: D002448:Cell Adhesion; D004730:Endothelium, Vascular / Q000187:drug effects / Q000503:physiopathology; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D008545:Melanoma / Q000378:metabolism* / Q000503:physiopathology; D016029:Receptors, Very Late Antigen / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D007375:Interleukin-1; D016029:Receptors, Very Late Antigen; D011994:Recombinant Proteins