Crystallization of the hybrid Bacillus (1-3,1-4)-beta-glucanase H(A16-M).
The extremely thermostable hybrid Bacillus (1-3,1-4)-beta-glucanase H(A16-M) has been crystallized with polyethylene glycol by vapour diffusion. The single crystals diffract to a resolution of 2.2 A. The protein crystallizes in the orthorhombic space group P2(1)2(1)2(1) with a = 70.22(7) A, b = 72.56(1) A, c = 49.97(1) A, and has one molecule per asymmetric unit.
Keitel
T
T
Institut für Kristallographie, Freie Universität Berlin.
Granzin
J
J
Simon
O
O
Borriss
R
R
Thomsen
K K
KK
Wessner
H
H
Höhne
W
W
Heinemann
U
U
D001407:Bacillus / Q000201:enzymology*; D006026:Glycoside Hydrolases / Q000737:chemistry*; D014961:X-Ray Diffraction
D006026:Glycoside Hydrolases; C028020:licheninase