Establishment of a human acute myeloid leukemia cell line (Kasumi-1) with 8;21 chromosome translocation.
A novel leukemic cell line with an 8;21 chromosome translocation, designated as Kasumi-1, was established from the peripheral blood of a 7-year-old boy suffering from acute myeloid leukemia (AML). The Kasumi-1 cells were positive for myeloperoxidase showing a morphology of myeloid maturation. The response in proliferation assay was observed in the culture with interleukin-3 (IL-3), IL-6, granulocyte colony-stimulating factor (G-CSF), and granulocytemacrophage CSF (GM-CSF), but not with IL-1 or IL-5. Neither granulocytic nor eosinophilic maturation was observed in the liquid culture by the addition of dimethyl sulfoxide, G-CSF, or IL-5, respectively. In contrast, induction of macrophagelike cells was seen by the addition of phorbol ester. This is the first report of a human AML cell line with t(8;21) that has characteristics of myeloid and macrophage lineages. The cell line could be a useful tool for elucidating the pathophysiology of AML with t(8;21).
Asou
H
H
Department of Hematology, Hiroshima University, Japan.
Tashiro
S
S
Hamamoto
K
K
Otsuji
A
A
Kita
K
K
Kamada
N
N
D015703:Antigens, CD / Q000032:analysis; D002454:Cell Differentiation; D002455:Cell Division; D002467:Cell Nucleus / Q000473:pathology; D002648:Child; D002891:Chromosomes, Human, Pair 21; D002898:Chromosomes, Human, Pair 8; D003593:Cytoplasm / Q000473:pathology; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D016130:Immunophenotyping; D007621:Karyotyping; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D008297:Male; D009693:Nucleic Acid Hybridization; D011994:Recombinant Proteins / Q000494:pharmacology; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D006133:Growth Substances; D011994:Recombinant Proteins