Microassay for DNA methyltransferase.
A microassay for DNA methylase is described which can detect activity in as few as 50 tissue culture cells. The cells are lysed and incubated for 2 h at 37 degrees C with 3 microCi high specific activity [3H]AdoMet and 0.5 microgram poly[d(I-C).d(I-C)] in a volume of 23 microliters. Ribonuclease is present during the assay and the product DNA is isolated by phenol extraction after protease digestion.
Adams
R L
RL
Department of Biochemistry, University of Glasgow, UK.
Rinaldi
A
A
Seivwright
C
C
D015254:DNA Modification Methylases / Q000032:analysis*; D007700:Kinetics; D008832:Microchemistry / Q000379:methods; D013379:Substrate Specificity
D015254:DNA Modification Methylases