Inhibition by putrescine of experimental carcinogenesis in rat colon induced by azoxymethane.
The effects of putrescine on the incidence and number of colon tumors induced by azoxymethane, and on the labelling index and the activity of ornithine decarboxylase (ODC) in the colon mucosa were investigated in Wistar rats. Rats received 10 weekly injections of 7.4 mg/kg body weight of azoxymethane and i.p. injections of 300 mumol/kg body weight of putrescine every 2 days until the end of the experiment at week 40. This prolonged treatment with putrescine significantly reduced the incidence and number of colon tumors. Administration of putrescine also significantly decreased the labelling index and the ODC activity in the colon mucosa during, but not after, treatment with the carcinogen. These last effects may be related to the action of putrescine in inhibiting the development of colonic tumors.
Tatsuta
M
M
Department of Gastrointestinal Oncology, Center for Adult Diseases, Osaka, Japan.
Iishi
H
H
Baba
M
M
Ichii
M
M
Nakaizumi
A
A
Uehara
H
H
Taniguchi
H
H
D000230:Adenocarcinoma / Q000139:chemically induced / Q000188:drug therapy; D000236:Adenoma / Q000139:chemically induced / Q000188:drug therapy; D000818:Animals; D001397:Azoxymethane / Q000037:antagonists & inhibitors; D001835:Body Weight / Q000187:drug effects; D002278:Carcinoma in Situ / Q000139:chemically induced / Q000188:drug therapy; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D008297:Male; D009955:Ornithine Decarboxylase / Q000096:biosynthesis; D011700:Putrescine / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D009955:Ornithine Decarboxylase; D001397:Azoxymethane; D011700:Putrescine