Biocompatibility of extracorporeal circulation. In vitro comparison of heparin-coated and uncoated oxygenator circuits.
Oxygenator/tubing sets coated with endpoint-attached heparin were compared to uncoated sets in a dynamic model of extracorporeal circulation. Biocompatibility was assessed by evaluations of complement activation and platelet loss. The median concentration of C3 activation products increased gradually from 12 AU/ml at baseline to 65 AU/ml after 120 minutes in the uncoated sets and from 12 AU/ml to 19 AU/ml after 120 minutes in the coated sets (p less than 0.002). The median concentration of the terminal complement complex in the uncoated sets increased gradually from 3.1 AU/ml at baseline to 18.2 AU/ml after 120 minutes. In the coated sets the terminal complement complex reached a peak of 12.0 AU/ml at 15 minutes and returned to baseline values at 60 minutes. Median platelet loss at 120 minutes was 166 x 10(9) in the uncoated and 21 x 10(9) in the coated sets (p less than 0.01). Heparin coating thus improved biocompatibility by reducing both complement activation and platelet loss.
Videm
V
V
Institute for Experimental Medical Research, Ullevaal Hospital, Oslo, Norway.
Mollnes
T E
TE
Garred
P
P
Svennevig
J L
JL
D001672:Biocompatible Materials; D003167:Complement Activation; D003176:Complement C3 / Q000276:immunology; D015938:Complement Membrane Attack Complex / Q000032:analysis; D005112:Extracorporeal Circulation / Q000295:instrumentation*; D006493:Heparin; D006801:Humans; D066298:In Vitro Techniques; D010976:Platelet Count
D001672:Biocompatible Materials; D003176:Complement C3; D015938:Complement Membrane Attack Complex; D006493:Heparin