title: Alterations in sugar residues in squamous metaplasia in hamster tracheal explants induced by benzo(a)pyrene and its reversal by retinoic acid.
abstract: We have demonstrated that squamous metaplasia induced by benzo(a)pyrene (BP) in the hamster tracheal explants accompany distinct alterations in carbohydrate moieties in the epithelial mucosa. Most prominent alterations were the preferential binding of peanut agglutinin (PNA) and wheat germ agglutinin (WGA) in the basal cell layer in metaplastic lesions. In this study we examined if reversal of BP-induced lesions by all-trans retinoic acid (RA) results in the acquisition of normal carbohydrate composition by the tissue. Four lectins, PNA, WGA, Dolichos biflorus agglutinin, and Concanavalin A, in their horseradish peroxidase conjugates were used. In control explants the intercellular plasma membrane of basal and mucous cells exhibited no significant reaction with any of the lectins tested. In the metaplastic lesions induced by BP, PNA and WGA intensely stained the plasma membrane and intercellular spaces of basal and intermediate cell layers; the granular layer cells did not bind PNA whereas they were stained moderately with WGA. RA, which reversed the metaplasia, also conferred the tissue with lectin binding patterns similar to that of control explants. These results thus show that the reversal of metaplasia is accompanied by acquisition of the tissue's original carbohydrate composition.
authors.affiliation: Institute of Chemical Toxicology, Wayne State University, Detroit, Michigan 48201.
authors.forename: D P
authors.initials: DP
authors.lastname: Chopra
authors.forename: A P
authors.initials: AP
authors.lastname: Joiakim
mesh_terms: D001564:Benzo(a)pyrene / Q000494:pharmacology*; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D006651:Histocytochemistry; D037102:Lectins / Q000378:metabolism; D008679:Metaplasia; D009924:Organ Culture Techniques; D014132:Trachea / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D037102:Lectins; D001564:Benzo(a)pyrene; D014212:Tretinoin