Pathological evaluation of anti-rheumatic drugs on type II collagen-induced arthritis in DBA/1J mouse.
The effects of anti-rheumatic drugs (lobenzarit (CCA); 10 and 50mg/kg, cyclophosphamide (CP); 5 mg/kg and dexamethasone (DM); 0.25mg/kg) were evaluated immunologically and histopathologically on DBA/1J mice that develop polyarthritis after immunization by the intradermal injection of type II collagen. Serum anti-type II collagen IgG levels in the groups treated with CP and DM were significantly suppressed to 1/2 and 1/10 as compared to those of the positive control group, respectively. Those of both groups treated with CCA were not different from those of the positive control group. Histopathological examination revealed that treatment with CP and DM markedly reduced or suppressed inflammatory changes and resulted in low incidence of arthritis. From the standpoint mentioned above, treatment with anti-rheumatic drugs suppressed the development of arthritis in this model, and we could confirm that this model was useful for evaluation of the effect of anti-rheumatic drugs.
Imaizumi
K
K
Safety Research Laboratory, Tanabe Seiyaku Co., Ltd., Osaka, Japan.
Hinoue
H
H
Ueno
M
M
Takata
I
I
Sato
T
T
Minato
Y
Y
Takeshita
M
M
Okaniwa
A
A
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use; D001168:Arthritis / Q000188:drug therapy / Q000276:immunology / Q000473:pathology*; D003094:Collagen / Q000008:administration & dosage / Q000276:immunology; D003520:Cyclophosphamide / Q000627:therapeutic use; D003907:Dexamethasone / Q000627:therapeutic use; D051379:Mice; D008811:Mice, Inbred DBA; D062367:ortho-Aminobenzoates / Q000627:therapeutic use
D000893:Anti-Inflammatory Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D062367:ortho-Aminobenzoates; D003907:Dexamethasone; D003520:Cyclophosphamide; D003094:Collagen; C015735:lobenzarit