Resolution of the conformational distribution and dynamics of a flexible molecule using frequency-domain fluorometry.
We report the first resolution of both the conformational distribution and end-to-end diffusion coefficient of a flexible molecule. This molecular information was recovered using only the donor intensity decay in a single solvent at a single viscosity, as observed by the technique of frequency-domain fluorometry. This technique can be extended to measurements of structural fluctuations of biological macromolecules.
Lakowicz
J R
JR
University of Maryland, School of Medicine, Department of Biological Chemistry, Baltimore 21201.
Kuśba
J
J
Wiczk
W
W
Gryczynski
I
I
Szmacinski
H
H
Johnson
M L
ML
D008433:Mathematics; D008962:Models, Theoretical; D008968:Molecular Conformation; D011336:Probability; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence / Q000379:methods