Cellular and subcellular localization of progastricsin in calf fundic mucosa: colocalization with pepsinogen and prochymosin.
The presence of gastricsin in bovine abomasal juice has been reported previously, but its exact site of origin has not yet been established. Specific polyclonal antibodies were used in the peroxidase-antiperoxidase method or the protein A/gold technique to label cells producing progastricsin. This immunocytolocalization was correlated with that of pepsinogen and prochymosin using specific polyclonal antibodies against those zymogens. The present study clearly established that progastricsin was located exclusively in chief, mucous neck, transitional mucous neck/chief, foveolar epithelial and surface epithelial cells of the calf fundic mucosa. Furthermore, progastricsin was found to be colocalized with pepsinogen and prochymosin in the same secretory granules of these cells. Progastricsin was not observed in parietal, gastric endocrine and undifferentiated neck cells.
Kurabayashi
Y
Y
Department of Veterinary Anatomy, Obihiro University of Agriculture and Veterinary Medicine, Japan.
Yamada
J
J
Andrén
A
A
Kitamura
N
N
Yamashita
T
T
D000018:Abomasum / Q000166:cytology / Q000201:enzymology*; D000818:Animals; D002417:Cattle; D012085:Chymosin / Q000032:analysis; D004792:Enzyme Precursors / Q000032:analysis; D005753:Gastric Mucosa / Q000166:cytology / Q000201:enzymology*; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D010435:Pepsinogens / Q000032:analysis*; D013347:Subcellular Fractions / Q000201:enzymology
D004792:Enzyme Precursors; D007106:Immune Sera; D010435:Pepsinogens; C032443:prorennin; D012085:Chymosin