Spectrophotometric method of inoculum preparation for the in vitro susceptibility testing of filamentous fungi.
Homogeneous inoculum suspensions of 29 isolates of clinically important filamentous fungi were adjusted with a spectrophotometer (530 nm) to obtain standardized preparations containing 1 x 10(6) to 5 x 10(6) CFU/ml. Colony counts (CFU per milliliter) of 1 x 10(6) to 5 x 10(6) were achieved on three different days for isolates of Aspergillus spp., Pseudallescheria boydii, and Sporothrix schenckii (80% +/- 2% transmission), and colony counts of 7 x 10(5) to 2.9 x 10(6) (70% +/- 2% transmission) were achieved for Mucor spp. and Rhizopus spp.
Espinel-Ingroff
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Division of Infectious Diseases, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0049.
Kerkering
T M
TM
D015169:Colony Count, Microbial; D005069:Evaluation Studies as Topic; D005658:Fungi / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods* / Q000592:standards; D013053:Spectrophotometry