Rearrangement and overexpression of D11S287E, a candidate oncogene on chromosome 11q13 in benign parathyroid tumors.
We report the detailed molecular characterization of a human parathyroid adenoma with a clonal parathyroid hormone gene rearrangement. This rearrangement is similar to one we characterized recently in an independent adenoma. In these two, plus a third partially characterized adenoma, one allele of the PTH gene, on 11p15, is rearranged with DNA from the D11S287 region on 11q13. This region contains a transcribed sequence, D11S287E, distinct from known 11q13 oncogenes, that is expressed in all parathyroid tissues examined, but is overexpressed dramatically in all three tumours with PTH gene-D11S287 rearrangements. These findings suggest that overexpression of D11S287E, perhaps driven by the misplaced PTH gene's regulatory elements, contributed to the development of these benign tumors. D11S287E is a new candidate oncogene with potential importance in parathyroid adenomas and perhaps other tumors with 11q13 abnormalities.
Rosenberg
C L
CL
Endocrine Unit, Massachusetts General Hospital, Boston 02114.
Kim
H G
HG
Shows
T B
TB
Kronenberg
H M
HM
Arnold
A
A
D000236:Adenoma / Q000235:genetics*; D015152:Blotting, Northern; D015139:Blotting, Southern; D002880:Chromosomes, Human, Pair 11; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D015321:Gene Rearrangement; D006801:Humans; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D010281:Parathyroid Hormone / Q000235:genetics; D010282:Parathyroid Neoplasms / Q000235:genetics*; D012334:RNA, Neoplasm / Q000032:analysis / Q000235:genetics; D015183:Restriction Mapping
D004273:DNA, Neoplasm; D010281:Parathyroid Hormone; D012334:RNA, Neoplasm