Stimulation of human dermal fibroblasts with interleukin 2.
The role of soluble inflammatory mediators in the wound healing process is under investigation. Interleukin 2 (IL2), an immune modulator produced by T lymphocytes, was tested in vitro for its effects on human diploid fibroblasts. Human diploid fibroblasts (1.0 x 10(4)) were incubated for 24 hours in Alpha minimal essential media (Gibco Laboratories, Gaithersburg, Md.) at 37 degrees C in 5% CO2 in air with 500 U/ml of IL2 (Cetus Corp., Emeryville, Calif.). Cells were then pulsed with 3 mu Ci/ml of tritiated thymidine overnight. The mean increase in the incorporation of tritiated thymidine of the cells treated with IL2 over control cells was 38% (p value less than 0.05). Interleukin 2 has a significant effect on the metabolism of human dermal fibroblasts and may accelerate the wound healing process.
Abdullah
A
A
University of Texas Medical Branch, Galveston.
McCauley
R L
RL
Herndon
D N
DN
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism; D012867:Skin; D013936:Thymidine / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology
D007376:Interleukin-2; D007930:Leucine; D013936:Thymidine