title: The phosphoenolpyruvate carboxylase gene family of Sorghum: promoter structures, amino acid sequences and expression of genes.
abstract: Two different members of the phosphoenolpyruvate carboxylase(PEPC)-encoding multigene family (clones lambda CP21 and lambda CP46) have been isolated from a Sorghum vulgare lambda EMBL4 genomic library. The use of the 3'-noncoding regions to probe Northern blots of RNA from roots, etiolated leaves and green leaves indicated that lambda CP21 and lambda CP46 encode the C3- and C4-type leaf PEPC isoforms, respectively. The lambda CP21 clone is expressed in the three tissues and is not light-regulated, whereas lambda CP46 is only expressed in greening leaves. The nucleotide sequence of the 5'-flanking DNA (520 bp) has been determined for both genes. For lambda CP46, several direct repeats were located in this region with similarities to sequences found in other light-regulated genes, but not in lambda CP21. The deduced amino acid sequences of the two S. vulgare PEPC proteins are 75% identical.
authors.affiliation: Laboratoire de Physiologie Végétale Moléculaire. Unité Associée CNRS-URA D1128, Université Paris-Sud. Orsay, France.
authors.forename: C
authors.initials: C
authors.lastname: Crétin
authors.forename: S
authors.initials: S
authors.lastname: Santi
authors.forename: E
authors.initials: E
authors.lastname: Keryer
authors.forename: L
authors.initials: L
authors.lastname: Lepiniec
authors.forename: D
authors.initials: D
authors.lastname: Tagu
authors.forename: J
authors.initials: J
authors.lastname: Vidal
authors.forename: P
authors.initials: P
authors.lastname: Gadal
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005091:Exons; D015870:Gene Expression; D017343:Genes, Plant; D015698:Genomic Library; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010730:Phosphoenolpyruvate Carboxylase / Q000235:genetics*; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D007527:Isoenzymes; D010730:Phosphoenolpyruvate Carboxylase