Long-term hypothermic storage of the cardiac explant. Comparison of four solutions.
This study compared the capabilities of 4 storage solutions in protecting the cardiac explant. Isolated rat heart was flushed with and stored in one of the storage solutions at 0 degrees C for 7 hours. The recovery of function was assessed using the working heart perfusion. Cardiac output returned to 34 +/- 6, 59 +/- 3 76 +/- 5, and 75 +/- 8% (mean +/- SE) of the control level in hearts stored in Euro-Collins, St. Thomas' Hospital cardioplegic, a modified University of Wisconsin (MUW) solutions, and a solution developed by us (CP-8), respectively. Therefore, MUW and CP-8 were superior to the other two solutions. During post-storage reperfusion, the coronary flow correlated directly with the aortic flow (r = 0.98), suggesting that preservation of coronary perfusion may be crucial to the recovery of function. There was also a good correlation between myocardial ATP levels and the cardiac output (r = 0.81). Thus, measurements towards the enhancement of ATP preservation and regeneration may be beneficial to the stored cardiac explant.
Wang
T
T
Department of Surgery, University of Rochester, School of Medicine and Dentistry, New York.
Batty
P R
PR
Hicks
G L
GL
DeWeese
J A
JA
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D001639:Bicarbonates; D002122:Calcium Chloride; D002302:Cardiac Output / Q000502:physiology; D002314:Cardioplegic Solutions; D003080:Cold Temperature; D016027:Heart Transplantation; D006982:Hypertonic Solutions; D008274:Magnesium; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000378:metabolism; D009926:Organ Preservation; D011189:Potassium Chloride; D051381:Rats; D011919:Rats, Inbred Strains; D012965:Sodium Chloride; D013997:Time Factors
D000227:Adenine Nucleotides; D001639:Bicarbonates; D002314:Cardioplegic Solutions; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; C041711:St. Thomas' Hospital cardioplegic solution; D012965:Sodium Chloride; D011189:Potassium Chloride; D008274:Magnesium; D002122:Calcium Chloride