Increased dithiothreitol-insensitive, type 2 angiotensin II receptors in selected brain areas of young rats.
1. Angiotensin II receptors have been studied by quantitative autoradiography in selected brain areas of young (2-week-old) and adult (8-week-old) rats. 2. In young rats, angiotensin II receptors were present in brain areas which did not express receptors in the adult brain, such as thalamic nuclei, cortical areas, and the cerebellum. 3. Young rats had more angiotensin II receptors in the subfornical organ than adult rats. In the inferior olive, the number of angiotensin receptors in young animals was 10 times higher than that in adult rats. Angiotensin II binding in the inferior olive was insensitive to incubation in the presence of dithiothreitol. 4. Conversely, the number of angiotensin II receptors in the nucleus of the solitary tract was lower in young rats compared to adults. Incubation in the presence of dithiothreitol resulted in a more than 90% inhibition of angiotensin II binding in the nucleus of the solitary tract. 5. Our results indicate the presence of two types of angiotensin II receptor in brain, one sensitive (type 1) and one insensitive (type 2) to the reducing agent dithiothreitol. 6. The expression of type 2 angiotensin II receptors, insensitive to dithiothreitol, is more marked in young rats, indicating a role for this type of angiotensin receptors in brain development.
Tsutsumi
K
K
Section on Pharmacology, National Institute of Mental Health, Bethesda, Maryland 20892.
Saavedra
J M
JM
D000367:Age Factors; D000818:Animals; D001066:Appetite / Q000502:physiology; D001921:Brain / Q000254:growth & development*; D001923:Brain Chemistry; D001931:Brain Mapping; D004229:Dithiothreitol / Q000494:pharmacology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011945:Receptors, Angiotensin / Q000032:analysis / Q000187:drug effects*; D012084:Renin-Angiotensin System / Q000502:physiology
D011945:Receptors, Angiotensin; D004229:Dithiothreitol