Comparative in vitro effects of liposomal amphotericin B, amphotericin B-deoxycholate, and free amphotericin B against fungal strains determined by using MIC and minimal lethal concentration susceptibility studies and time-kill curves.
Multilamellar liposomal amphotericin B (L-AmB) was generally less active in vitro against yeast strains than was amphotericin B-deoxycholate or free amphotericin B, although continual agitation of the broth disproportionately increased the activity of L-AmB. Time-kill studies also demonstrated a slower onset of action of L-AmB and supported the hypothesis that liposomes may act as reservoirs for free amphotericin B, which is the active moiety.
Ralph
E D
ED
Department of Medicine, University Hospital, London, Ontario, Canada.
Khazindar
A M
AM
Barber
K R
KR
Grant
C W
CW
D000666:Amphotericin B / Q000008:administration & dosage / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects*; D003455:Cryptococcus neoformans / Q000187:drug effects*; D003840:Deoxycholic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D004337:Drug Carriers; D004338:Drug Combinations; D008081:Liposomes; D008826:Microbial Sensitivity Tests; D013997:Time Factors
D004337:Drug Carriers; D004338:Drug Combinations; D008081:Liposomes; D003840:Deoxycholic Acid; D000666:Amphotericin B; C059765:amphotericin B, deoxycholate drug combination