High-performance liquid chromatographic determination of plasma glycolic acid in healthy subjects and in cases of hyperoxaluria syndromes.
A liquid chromatographic procedure for the determination of glycolic acid in plasma is proposed. The system is based on pre-column derivatization of the alpha-keto acid by means of phenylhydrazine, coupled with the enzymatic oxidation of glycolate to glyoxylate. The phenylhydrazone formed is separated by reversed-phase liquid chromatography and detected by UV absorption. The measured within and between-batch CV imprecision was 2.6 and 11.3%, respectively, at 5.68 mumol/l glycolate concentration; the analytical recovery was 102.0 +/- 7.3% and the minimum detectable concentration of glycolate was 0.3 mumol/l. The reference interval for plasma glycolate was 4.51 to 12.20 mumol/l (n = 14). Results of determinations of plasma samples from uremic patients, patients with type I primary hyperoxaluria and patients with chronic renal failure secondary to systemic oxalosis are reported.
Petrarulo
M
M
Laboratory of Renal Stone Disease, Ospedale Mauriziano Umberto, Turin, Italy.
Marangella
M
M
Linari
F
F
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006016:Glycolates / Q000097:blood*; D006801:Humans; D006835:Hydrazones / Q000032:analysis; D006960:Hyperoxaluria, Primary / Q000097:blood* / Q000150:complications; D007676:Kidney Failure, Chronic / Q000097:blood; D007700:Kinetics; D006435:Renal Dialysis; D014462:Ultrafiltration; D014511:Uremia / Q000378:metabolism; D026681:Vitamin B 6 Deficiency / Q000097:blood
D006016:Glycolates; D006835:Hydrazones; C012483:phenylhydrazone; C031149:glycolic acid