title: African swine fever virus attachment protein.
abstract: Treatment of African swine fever virus particles with nonionic detergents released proteins p35, p17, p14, and p12 from the virion. Of these proteins, only p12 bound to virus-sensitive Vero cells but not to virus-resistant L or IBRS2 cells. The binding of p12 was abolished by whole African swine fever virus and not by similar concentrations of subviral particles that lacked the external proteins. A monoclonal antibody (24BB7) specific for p12 precipitated a protein that, when analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the absence of 2-mercaptoethanol, showed a molecular mass of 17 kDa (p17*) instead of 12 kDa as found in the presence of 2-mercaptoethanol. The relationship between these two proteins was confirmed by the conversion of p17* to p12 when the former was isolated from polyacrylamide gels in the absence of 2-mercaptoethanol and subsequently treated with the reducing agent. The supernatant obtained after immunoprecipitation with the p12-specific antibody lacked the virus-binding protein.
authors.affiliation: Centro de Biología Molecular (Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid, Facultad de Ciencias, Spain.
authors.forename: A L
authors.initials: AL
authors.lastname: Carrascosa
authors.forename: I
authors.initials: I
authors.lastname: Sastre
authors.forename: E
authors.initials: E
authors.lastname: Viñuela
mesh_terms: D000358:African Swine Fever Virus / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D005960:Glucosides / Q000378:metabolism; D008623:Mercaptoethanol / Q000494:pharmacology; D009500:Neutralization Tests; D011991:Receptors, Virus / Q000378:metabolism; D014709:Vero Cells; D014760:Viral Fusion Proteins / Q000276:immunology / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D003902:Detergents; D005960:Glucosides; D011991:Receptors, Virus; D014760:Viral Fusion Proteins; C018619:octyl-beta-D-glucoside; D008623:Mercaptoethanol