Phenylalanine in whole blood and plasma--a candidate reference method.
A method for the quantification of phenylalanine in whole blood and plasma by isotope dilution gas chromatography-mass spectrometry is presented. The use of an uncommon derivative allows a simple extraction procedure and the most basic mass spectrometer. The relative standard deviation was found to be 1.3% within-batch and 2.1% between-batch under optimum conditions, and the detection limit was found to be 7 pmol injected. The ruggedness of the procedure and sample handling conditions were also examined.
Lynes
G W
GW
Department of Clinical Biochemistry, Hospitals for Sick Children, London, U.K.
Hjelm
M
M
D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007202:Indicators and Reagents; D010649:Phenylalanine / Q000097:blood*; D012015:Reference Standards
D007202:Indicators and Reagents; D010649:Phenylalanine