title: DNA lesion in rat hepatocytes induced by in vitro and in vivo exposure to glyoxal.
abstract: The alkaline elution technique was applied to measure the damage of rat hepatic DNA following exposure to glyoxal. DNA single-strand breaks were induced after exposure of primary-cultured hepatocytes to 0.1-0.6 mg/ml glyoxal for 60 min, while no DNA cross-link was observed. Single-strand breaks were also detected in livers of rats within 2 h following a single oral exposure at 200-1000 mg/kg body weight, and the frequency of the breaks reached a maximum around 9 h after exposure. The breaks were almost fully repaired 24 h after exposure to any dose. However, hardly any DNA lesions were detected in other tissues following exposure to 1000 mg/kg glyoxal. Thus, the present results indicate that glyoxal causes DNA single-strand breaks in rat hepatocytes following in vitro and in vivo exposure.
authors.affiliation: Division of Environmental Health, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ueno
authors.forename: K
authors.initials: K
authors.lastname: Nakamuro
authors.forename: Y
authors.initials: Y
authors.lastname: Sayato
authors.forename: S
authors.initials: S
authors.lastname: Okada
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D004260:DNA Repair / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006037:Glyoxal / Q000633:toxicity*; D008099:Liver / Q000187:drug effects*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D006037:Glyoxal; D004247:DNA