Ferric-salicylaldehyde isonicotinoyl hydrazone, a synthetic iron chelate, alleviates defective iron utilization by reticulocytes of the Belgrade rat.
The Belgrade rat has a hypochromic, microcytic anemia inherited as an autosomal recessive mutation. Although transferrin binds normally to reticulocytes and internalizes normally, iron accumulation into cells and heme is much slower than normal. We have investigated the role of the transferrin cycle in this mutant by bypassing transferrin iron delivery with the iron chelate ferric salicylaldehyde isonicotinoyl hydrazone (Fe-SIH). Fe-SIH increases iron uptake into heme by Belgrade reticulocytes, restoring it almost to normal levels. This increase indicates that Fe-SIH delivers iron to a step in iron utilization that is after the Belgrade defect. Depleting reticulocytes of transferrin did not alter these observations. Failure to achieve above normal rates of iron incorporation could indicate damage due to chronic intracellular iron deficiency. Also, iron delivery by Fe-SIH restored globin synthesis to near-normal levels in Belgrade reticulocytes. The rates of glycine incorporation into porphyrin and heme in Belgrade reticulocytes incubated with Fe2-transferrin or Fe-SIH paralleled the rates of iron incorporation into heme. These data are consistent with the concept that iron availability limits protoporphyrin formation in rat reticulocytes. The protoporphyrin used for heme synthesis is provided by de novo synthesis and not by a pool of pre-existing protoporphyrin. The Belgrade defect occurs in the movement of iron from transferrin to a step prior to the ferrous state and insertion into heme. This defect diminishes the synthesis of heme and, consequently, that of protoporphyrin and globin.
Garrick
L M
LM
Department of Biochemistry, State University of New York, Buffalo 14214.
Gniecko
K
K
Hoke
J E
JE
al-Nakeeb
A
A
Ponka
P
P
Garrick
M D
MD
D000818:Animals; D002478:Cells, Cultured; D005914:Globins / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D006418:Heme / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000494:pharmacology*; D007538:Isoniazid / Q000031:analogs & derivatives* / Q000494:pharmacology; D011166:Porphyrins / Q000378:metabolism; D051381:Rats; D012156:Reticulocytes / Q000378:metabolism*; D014168:Transferrin / Q000378:metabolism / Q000502:physiology
D007502:Iron Chelating Agents; D011166:Porphyrins; D014168:Transferrin; C049137:salinazid; D006418:Heme; D005914:Globins; D007501:Iron; D005998:Glycine; D007538:Isoniazid