title: Quantitative comparison of magnetic resonance imaging (MRI) and histologic analyses of focal ischemic damage in the rat.
abstract: Hemispheric swelling and area of infarction, two parameters of cerebral focal ischemic damage, were identified and quantified from T2-weighted magnetic resonance imaging (MRI) two days after occlusion of the middle cerebral artery (MCAO) in spontaneously hypertensive rat (SHR) brains. Results were compared with these measures quantified from 2,3,5-triphenyltetrazolium hydrochloride (TTC)- and hematoxylin and eosin (H&E)-stained histologic sections in the same brains. The degree of hemispheric swelling and infarct size determined by MRI were highly correlated to the measurements as determined in the TTC- and H&E-stained tissues. These results demonstrate that the focal ischemic damaged area and associated tissue swelling identified by MRI is quantitatively similar to, and thus, is representative of actual tissue damage/changes that can be identified by gross or histologic examination.
authors.affiliation: Department of Pharmacology, SmithKline Beecham Pharmaceuticals, plc, King of Prussia, PA 19406.
authors.forename: F C
authors.initials: FC
authors.lastname: Barone
authors.forename: R K
authors.initials: RK
authors.lastname: Clark
authors.forename: G
authors.initials: G
authors.lastname: Feuerstein
authors.forename: R E
authors.initials: RE
authors.lastname: Lenkinski
authors.forename: S K
authors.initials: SK
authors.lastname: Sarkar
mesh_terms: D000818:Animals; D002544:Cerebral Infarction / Q000175:diagnosis; D002546:Ischemic Attack, Transient / Q000175:diagnosis*; D008279:Magnetic Resonance Imaging; D051381:Rats; D011918:Rats, Inbred SHR