Induction of a diffuse mesothelioma in chickens by intraperitoneal inoculation of v-src DNA.
The presence of peritoneum-based, cytokeratin-positive tumor cells, as detected under conditions of intravenous inoculation of chickens with a v-src-positive DNA fragment, raised the possibility that such DNA may be inductive for diffuse mesothelioma. To investigate this possibility, chickens were inoculated intraperitoneally with v-src DNA, and the resultant peritoneum-based tumor tissue was subjected to histopathological analysis. On the basis of (i) morphology, (ii) immunohistochemistry/histochemistry, and (iii) ultrastructure, we concluded that this tissue represents a diffuse mesothelioma.
England
J M
JM
Department of Pathology and Laboratory Medicine, Medical College of Pennsylvania, Philadelphia 19129.
Panella
M J
MJ
Ewert
D L
DL
Halpern
M S
MS
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002645:Chickens; D004279:DNA, Viral / Q000235:genetics; D016391:Genes, src; D007274:Injections, Intraperitoneal; D008654:Mesothelioma / Q000235:genetics* / Q000473:pathology; D015688:Oncogene Protein pp60(v-src) / Q000235:genetics*
D004279:DNA, Viral; D015688:Oncogene Protein pp60(v-src)