The complete amino acid sequence of feline beta-lactoglobulin II and a partial revision of the equine beta-lactoglobulin II sequence.
The amino acid sequence of feline beta-lactoglobulin (designated II) has been determined. The protein chain is 163 amino acids long with a relative molecular mass of 18,558. The primary structure was determined by sequencing of native protein (residues 1-25), BPNS-skatole cleavage fragments and the peptides obtained by proteolytic cleavage with V8 proteinase and TPCK-trypsin. Feline beta-lactoglobulin II has 53 and 57% positional identities with bovine beta-lactoglobulin A and equine beta-lactoglobulin I, respectively, and approx. 68% with a revised sequence of equine beta-lactoglobulin II. The equine beta-lactoglobulin II sequence was re-examined between positions 78 and 122 resulting in a major revision in this area with only a single insertion to give a total of 163 residues.
Halliday
J A
JA
Department of Physiology and Pharmacology, University of Queensland, Brisbane, Australia.
Bell
K
K
Shaw
D C
DC
D000595:Amino Acid Sequence; D000818:Animals; D002415:Cats; D002417:Cattle; D006041:Goats; D006736:Horses; D007782:Lactoglobulins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
D007782:Lactoglobulins