title: Alteration of the T-cell receptor repertoire in A.CA mice expressing an Ead transgene.
abstract: In an effort to generate an A.CA mouse expressing Ed, the Ead gene has been introduced into A.CA mice which lack the major histocompatibility complex (MHC) class II E molecule. Flow cytometric analysis shows cell surface expression of the E alpha chain on lymphocytes and macrophages in the transgenic mice. Analysis of T-cell receptor (Tcr) genes deleted in some E-expressing mouse strains demonstrates that T cells expressing Tcrb-V5 are partially deleted in these transgenic mice while those expressing Tcrb-V8 and Tcrb-11 are not. In addition, the expressed E alpha d chain can promote Mycoplasma arthriditis mitogen (MAM)-induced T-cell proliferation. The expression of the E alpha chain, presumably as an A beta fE alpha d heterodimer, can alter the peripheral T-cell repertoire and T-cell reactivity to a microbial superantigen.
authors.affiliation: Department of Microbiology & Immunology, Albert Einstein College of Medicine, Bronx, NY 10461.
authors.forename: S
authors.initials: S
authors.lastname: Ishikawa
authors.forename: M Y
authors.initials: MY
authors.lastname: Chang
authors.forename: B
authors.initials: B
authors.lastname: Diamond
mesh_terms: D000818:Animals; D002872:Chromosome Deletion; D006183:H-2 Antigens / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D006183:H-2 Antigens; D011948:Receptors, Antigen, T-Cell