Absence of mutagenic action of 5 beta-cholan-24-oic acid derivatives in the bacterial fluctuation and standard Ames tests.
Published data on the mutagenicity of 3 bile acids in the bacterial fluctuation test are conflicting. Eleven 5 beta-cholanoic acids including 2 of the bile acids were assayed for mutagenicity in Salmonella typhimurium TA98 and TA100 in the fluctuation tests. In any of these bile acids at the doses tested, there were no dose-related statistically significant increases in mutagenicity compared with appropriate controls. Similarly, none of these compounds showed positive mutagenicity in both strains in the standard Ames test either with or without hepatic metabolic activation. Our results support the claim that 3 bile acids are not mutagenic, and indicate that the initiation activity of 5 beta-cholanoic acids is not demonstrable with a short-term assay using Salmonella strains.
Mori
Y
Y
Radioisotope Laboratory, Gifu Pharmaceutical University, Japan.
Nii
H
H
Masuda
A
A
Mizumoto
K
K
Konishi
Y
Y
Une
M
M
Hoshita
T
T
D000818:Animals; D001711:Biotransformation; D019826:Cholic Acid; D002793:Cholic Acids / Q000008:administration & dosage / Q000494:pharmacology / Q000633:toxicity; D003840:Deoxycholic Acid / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009152:Mutagenicity Tests; D051381:Rats; D011919:Rats, Inbred Strains; D012486:Salmonella typhimurium / Q000187:drug effects
D002793:Cholic Acids; D003840:Deoxycholic Acid; D019826:Cholic Acid; C007036:cholanic acid