title: Leukotriene C4 induces mesenteric hypoperfusion and intestinal intramural acidosis in pigs.
abstract: We examined the effect of intravenous infusion of graded doses of authentic leukotriene (LT) C4 on several physiological variables in pentobarbital-anesthetized immature swine. Mesenteric blood flow (Qsma) was measured using an ultrasonic flow probe and ileal intramucosal hydrogen ion concentration ([H+]I) was estimated tonometrically. Three groups were studied. Pigs in Group I (n = 6) were infused beginning at t = 0 min with increasing doses (0.03-1.0 microgram/kg-min) of LTC4, each dose being administered for 10 min. Pigs in Group II (n = 6) were infused with LTC4 as above, but were pre- and post-treated with a specific sulfidopeptide LT receptor antagonist, LY203647 (30 mg/kg bolus and then 10 mg/kg-hr) beginning at t = -20 min. Pigs in Group III (n = 4) received only normal saline (5 ml/kg-h). Infusing LTC4 significantly decreased Qsma and mesenteric oxygen uptake and significantly increased ileal [H+]I. These changes were prevented by LY203647. These data support the idea that sulfido-peptide LT are capable of causing mesenteric ischemia and that this phenomenon can be blocked by LY203647.
authors.affiliation: University of Massachusetts Medical Center, Worcester 01605.
authors.forename: S M
authors.initials: SM
authors.lastname: Cohn
authors.forename: K L
authors.initials: KL
authors.lastname: Kruithoff
authors.forename: H R
authors.initials: HR
authors.lastname: Rothschild
authors.forename: H L
authors.initials: HL
authors.lastname: Wang
authors.forename: J B
authors.initials: JB
authors.lastname: Antonsson
authors.forename: S O
authors.initials: SO
authors.lastname: Heard
authors.forename: M P
authors.initials: MP
authors.lastname: Fink
mesh_terms: D000098:Acetophenones / Q000494:pharmacology; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D008297:Male; D010100:Oxygen / Q000097:blood; D010101:Oxygen Consumption / Q000187:drug effects; D013189:SRS-A / Q000494:pharmacology*; D013152:Splanchnic Circulation / Q000187:drug effects*; D013552:Swine; D013777:Tetrazoles / Q000494:pharmacology
chemical_list: D000098:Acetophenones; D013189:SRS-A; D013777:Tetrazoles; C061324:LY 203647; D010100:Oxygen