Effects of thymidine and uridine on the phosphorylation of 3'-azido-3'-deoxythymidine (zidovudine) in human mononuclear cells.
The effects of thymidine and uridine on the phosphorylation of 3'-azido-3'-deoxythymidine (AZT) were studied in various human mononuclear cell preparations. Thymidine suppressed [3H]AZT phosphorylation in the same concentration range (20 to 100 microM) in which it antagonizes the anti-human immunodeficiency virus activity of AZT. Uridine, in turn, had no influence on AZT phosphorylation, just as it has no effect on the anti-human immunodeficiency virus activity of AZT. These findings are consistent with a close relationship between the inhibition of AZT phosphorylation and the influence of physiological nucleosides on the antiviral activity of AZT.
Szebeni
J
J
National Cancer Institute, Bethesda, Maryland 20892.
Patel
S S
SS
Hung
K
K
Wahl
L M
LM
Weinstein
J N
JN
D001853:Bone Marrow / Q000378:metabolism; D001854:Bone Marrow Cells; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D010766:Phosphorylation; D013936:Thymidine / Q000494:pharmacology*; D013937:Thymidine Kinase / Q000378:metabolism; D014316:Tritium; D014529:Uridine / Q000494:pharmacology*; D015215:Zidovudine / Q000097:blood* / Q000378:metabolism
D014316:Tritium; D015215:Zidovudine; D013937:Thymidine Kinase; D013936:Thymidine; D014529:Uridine