HIV antigen detection in circulating immune complexes.
Various methods were evaluated for their effectiveness in releasing HIV antigen (Ag) from artificial immune complexes (IC) and from IC present in serum from HIV antibody (Ab) positive subjects. The most effective methods for recovering HIV Ag from IC were those which included a denaturation step to prevent reassociation of Ag with Ab. IC precipitation in 2.5% polyethylene glycol followed by acid treatment with 1 M glycine.HCl (pH 2) for 10 min at 70 degrees C in the presence of 0.05% SDS gave very satisfactory results. With this method, IC were detected in sera from HIV antibody positive Caucasian subjects at all stages of infection. After HIV IC dissociation, HIV Ag was detected in a significant number (8/17 or 47%) of asymptomatic subjects. IC were most prevalent during the late stages of infection. A substantial increase in HIV Ag positivity was also observed in 20 Senegalese HIV Ab positive sera. After HIV IC dissociation HIV antigen detection increased from 2/20 to 12/20. The relevance of IC detection is discussed.
Kestens
L
L
Institute of Tropical Medicine, Antwerpen, Belgium.
Hoofd
G
G
Gigase
P L
PL
Deleys
R
R
van der Groen
G
G
D000936:Antigen-Antibody Complex / Q000737:chemistry*; D016009:Chi-Square Distribution; D015488:HIV Antigens / Q000032:analysis*; D006801:Humans; D008722:Methods
D000936:Antigen-Antibody Complex; D015488:HIV Antigens