Specific binding of antigenic peptides to cell-associated MHC class I molecules.
T lymphocytes recognize antigen in the form of peptides that associate with specific alleles of class I or class II major histocompatibility (MHC) molecules. By contrast with the clear MHC allele-specific binding of peptides to purified class II molecules purified solubilized class I molecules either bind relatively poorly or show degenerate specificity. Using photo-affinity labelling, we demonstrate here the specific interaction of peptides with cell-associated MHC class I molecules and show that this involves metabolically active processes.
Luescher
I F
IF
Ludwig Institute for Cancer Research, Lausanne Branch, Epalinges, Switzerland.
Romero
P
P
Cerottini
J C
JC
Maryanski
J L
JL
D000345:Affinity Labels; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000276:immunology*; D001667:Binding, Competitive; D002460:Cell Line; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D007700:Kinetics; D007739:L Cells / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D010962:Plasmodium berghei / Q000276:immunology*; D011485:Protein Binding; D015800:Protozoan Proteins; D013601:T-Lymphocytes / Q000276:immunology*
D000345:Affinity Labels; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D015395:Histocompatibility Antigens Class I; D015800:Protozoan Proteins; C041595:circumsporozoite protein, Protozoan