Inhibition of phosphoenolpyruvate carboxykinase by 6-phosphogluconate in rat liver.
Various concentrations of 6-phosphogluconate inhibit rat liver phosphoenolpyruvate carboxykinase activity. 0.04 mM 6-phosphogluconate, which is the concentration found in vivo, caused a 50% inhibition of 6-phosphoenolpyruvate carboxykinase activity. 6-Phosphogluconate lowered the Vmax of the enzyme and increased the concentration of phosphoenolpyruvate required to achieve one-half of the maximum velocity. The role of 6-phosphogluconate as a regulator of the coordination of fluxes through three metabolic pathways is discussed.
Kaloyianni
M
M
Laboratory of Animal Physiology, Science School, Aristotelian University of Thessaloniki, Greece.
D000818:Animals; D005942:Gluconates / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008297:Male; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000037:antagonists & inhibitors*; D051381:Rats; D011919:Rats, Inbred Strains
D005942:Gluconates; D010729:Phosphoenolpyruvate Carboxykinase (GTP); C008884:6-phosphogluconic acid