Apoptosis as a mechanism of cell death in cultured T lymphoblasts acutely infected with HIV-1.
The mechanisms by which HIV-1 infection kills T lymphocytes are not clearly established. Apoptosis is an internally programmed cell death pathway that may regulate both T cell development and senescence, and that is characterized by cleavage of DNA at internucleosomal regions. The present experiments show that acute HIV-1 infection of MT2 lymphoblasts and activated normal peripheral blood mononuclear cells induces apoptosis. The addition of anti-gp120 neutralizing antibody, after HIV-1 infection of MT2 cells, permitted sustained high levels of viral replication, but blocked apoptosis and cell death. Apoptosis may account for the direct cytopathologic effects of HIV-1 in T cells.
Terai
C
C
Department of Medicine, University of California at San Diego, La Jolla 92093.
Kornbluth
R S
RS
Pauza
C D
CD
Richman
D D
DD
Carson
D A
DA
D015704:CD4 Antigens / Q000502:physiology; D002118:Calcium / Q000378:metabolism; D002470:Cell Survival; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D015699:HIV Envelope Protein gp120 / Q000378:metabolism; D015658:HIV Infections / Q000473:pathology; D015497:HIV-1 / Q000502:physiology; D006801:Humans; D009707:Nucleosomes / Q000378:metabolism; D013601:T-Lymphocytes / Q000382:microbiology / Q000473:pathology*
D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120; D009707:Nucleosomes; D004247:DNA; D002118:Calcium