title: A novel cDNA clone encoding a prolactin-like protein that lacks the two C-terminal cysteine residues isolated from bovine placenta.
abstract: A new prolactin-like cDNA clone, bPLP-IV, was isolated from a bovine placental cDNA library and the complete nucleotide sequence was determined. The bPLP-IV encodes a protein consisting of 237 amino acids, which is related to, but different from seven other known bovine prolactin-like proteins including two placental lactogens. The predicted amino acid sequence of the bPLP-IV shows over 52% identity to other known members of bovine prolactin-like proteins, 48% to bovine prolactin, 40% to both two bovine placental lactogens and only 22% to bovine growth hormone. The bPLP-IV protein has a unique feature in its primary structure, lacking the two C-terminal cysteine residues which are completely conserved in all other known members of prolactin-growth hormone-placental lactogen gene family. The expression of bPLP-IV in developing bovine placenta was apparently stage-specific, being maximal in the full-term placenta.
authors.affiliation: Department of Biochemistry, Mie University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.forename: H
authors.initials: H
authors.lastname: Minoura
authors.forename: H
authors.initials: H
authors.lastname: Ushiro
authors.forename: K
authors.initials: K
authors.lastname: Nakashima
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002417:Cattle; D003545:Cysteine / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D005260:Female; D008969:Molecular Sequence Data; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D015183:Restriction Mapping; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
chemical_list: D011257:Pregnancy Proteins; D012333:RNA, Messenger; C068335:prolactin-like protein IV; D004247:DNA; D003545:Cysteine