Inhibition of cell division in hupA hupB mutant bacteria lacking HU protein.
Escherichia coli hupA hypB double mutants that lack HU protein have severe cellular defects in cell division, DNA folding, and DNA partitioning. Here we show that the sfiA11 mutation, which alters the SfiA cell division inhibitor, reduces filamentation and production of anucleate cells in AB1157 hupA hupB strains. However, lexA3(Ind-) and sfiB(ftsZ)114 mutations, which normally counteract the effect of the SfiA inhibitor, could not restore a normal morphology to hupA hupB mutant bacteria. The LexA repressor, which controls the expression of the sfiA gene, was present in hupA hupB mutant bacteria in concentrations half of those of the parent bacteria, but this decrease was independent of the specific cleavage of the LexA repressor by activated RecA protein. One possibility to account for the filamentous morphology of hupA hupB mutant bacteria is that the lack of HU protein alters the expression of specific genes, such as lexA and fts cell division genes.
Dri
A M
AM
Enzymologie, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
Rouviere-Yaniv
J
J
Moreau
P L
PL
D001426:Bacterial Proteins / Q000096:biosynthesis; D002455:Cell Division; D004247:DNA / Q000096:biosynthesis; D004268:DNA-Binding Proteins / Q000502:physiology*; D004926:Escherichia coli / Q000502:physiology*; D029968:Escherichia coli Proteins; D005809:Genes, Regulator; D008856:Microscopy, Fluorescence; D011938:Rec A Recombinases / Q000096:biosynthesis; D012697:Serine Endopeptidases; D000099160:LexA Repressor Protein
D001426:Bacterial Proteins; D004247:DNA; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D011938:Rec A Recombinases; D012697:Serine Endopeptidases; D000099160:LexA Repressor Protein; C040330:histone-like protein HU, bacteria; C053139:sulA protein, E coli