Effect of plasma triglyceride concentrations on the accuracy of immunoturbidimetric assays of apolipoprotein B.
We have studied the influence of triglyceride-rich particles on the analytical bias of apolipoprotein B measurements by various immunoturbidimetric methods. Three commercially available methods grossly overestimate apolipoprotein B in samples with even moderately above-normal triglyceride concentrations. This effect is due to the increased relative reactivity of very-low-density lipoproteins in these reagent systems, and can be eliminated by including Tween 20 (2 g/L) in the reagent buffer. We have developed, and describe, an automated immunoturbidimetric method that allows the accurate determination of apolipoprotein B in the plasma of patients with hypertriglyceridemia.
Walmsley
T A
TA
Biochemistry Department, Princess Margaret Hospital, Christchurch, New Zealand.
Grant
S
S
George
P M
PM
D001055:Apolipoproteins B / Q000097:blood*; D002784:Cholesterol / Q000097:blood; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D009391:Nephelometry and Turbidimetry / Q000379:methods; D011136:Polysorbates; D012016:Reference Values; D015203:Reproducibility of Results; D014280:Triglycerides / Q000097:blood*; D014461:Ultracentrifugation
D001055:Apolipoproteins B; D008079:Lipoproteins, VLDL; D011136:Polysorbates; D014280:Triglycerides; D002784:Cholesterol