title: Use of synthetic peptides to locate neutralizing antigenic domains on the fusion protein of respiratory syncytial virus.
abstract: Chemical and enzymic cleavages of the F1 subunit of the fusion (F) protein of respiratory syncytial (RS) virus showed that the sequence 184-Gly to 314-Trp reacted with neutralizing monoclonal antibodies (MAbs). Twelve synthetic peptides covering a part of this sequence were analysed for their immunoreactivity with neutralizing MAbs and anti-RS virus rabbit serum. Two sequential antigenic domains corresponding to amino acids 200 to 225 and 255 to 278 were defined with anti-RS virus rabbit serum. The peptides 205-225 and 259-278, belonging to these antigenic domains, inhibited binding to the F protein and the neutralizing activity of the anti-RS virus rabbit serum. One MAb (RS-348) reacted with peptides containing amino acids 200 to 225. Moreover, the peptide 205-225 induced an anti-peptide rabbit serum neutralizing RS virus in vitro. These results indicate that the sequence from residues 200 to 225 was present in one of the immunodominant sites of the F protein.
authors.affiliation: Laboratory of Virology, Faculty de Médecine Dijon, France.
authors.forename: C
authors.initials: C
authors.lastname: Bourgeois
authors.forename: C
authors.initials: C
authors.lastname: Corvaisier
authors.forename: J B
authors.initials: JB
authors.lastname: Bour
authors.forename: E
authors.initials: E
authors.lastname: Kohli
authors.forename: P
authors.initials: P
authors.lastname: Pothier
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000235:genetics* / Q000276:immunology*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D015707:HN Protein; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000276:immunology; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins / Q000235:genetics / Q000276:immunology*; D014764:Viral Proteins
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D015707:HN Protein; D010446:Peptide Fragments; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D014764:Viral Proteins; C116204:attachment protein G