title: High-velocity mechanical DNA transfer of the chloramphenicolacetyl transferase gene into rodent liver, kidney and mammary gland cells in organ explants and in vivo.
abstract: Mouse and rat liver, kidney and mammary gland explants were bombarded with high-velocity microprojectiles carrying a chloramphenicolacetyl transferase gene under different promoters (pTAT-cat, p chi-Casein-cat, p beta-Casein-cat). The expression of a CAT gene was revealed in all organ explants 24 h after transfection. The most pronounced expression was found when a TAT-CAT construction was used. In experiments in vivo rat liver was bombarded in situ with microprojectiles carrying pTAT-cat DNA. A marked activity of the CAT gene was detected 24 h after the bombardment.
authors.affiliation: Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
authors.forename: A V
authors.initials: AV
authors.lastname: Zelenin
authors.forename: A A
authors.initials: AA
authors.lastname: Alimov
authors.forename: A V
authors.initials: AV
authors.lastname: Titomirov
authors.forename: A V
authors.initials: AV
authors.lastname: Kazansky
authors.forename: S I
authors.initials: SI
authors.lastname: Gorodetsky
authors.forename: V A
authors.initials: VA
authors.lastname: Kolesnikov
mesh_terms: D000818:Animals; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics*; D004247:DNA / Q000737:chemistry; D066298:In Vitro Techniques; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008321:Mammary Glands, Animal / Q000201:enzymology*; D011401:Promoter Regions, Genetic; D051381:Rats; D011919:Rats, Inbred Strains; D014162:Transfection; D014170:Transformation, Genetic
chemical_list: D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase