title: Flow cytometric analysis of nuclear DNA content in advanced gastric cancer and its relationship with prognosis.
abstract: Cell nuclear DNA content was determined by flow cytometric analysis in 270 patients with deeply infiltrating (beyond the submucosa) cancer, so-called advanced gastric cancer. Aneuploidy was observed in 150 cases (55.6%). Multivariate analysis showed that the DNA ploidy pattern was the third significant prognostic factor behind peritoneal dissemination and liver metastasis. The 5-year survival rate of diploid patients (62.9%) was significantly higher than that of aneuploid patients (22.7%) (P less than 0.01). This trend was most clear in patients with Stage III and IV tumors. Among aneuploid cases the high DNA index group indicated a worse prognosis than the low DNA index group. It was thus assumed that the DNA ploidy pattern was a useful prognostic indicator of advanced gastric cancer.
authors.affiliation: Department of Surgery II, School of Medicine, Kanazawa University, Ishikawa, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kimura
authors.forename: Y
authors.initials: Y
authors.lastname: Yonemura
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008113:Liver Neoplasms / Q000556:secondary; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011003:Ploidies; D011379:Prognosis; D013274:Stomach Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D015996:Survival Rate
chemical_list: D004273:DNA, Neoplasm