A fibroblast-specific factor binds to an upstream negative control element in the promoter of the mouse alpha 1(I) collagen gene.
We have identified, in nuclear extracts of NIH-3T3 fibroblasts, a factor which binds a sequence between -339 and -361 in the mouse alpha 1(I) collagen promoter. A 3-base pair substitution mutation introduced in this promoter element, which abolishes the binding of the factor, increases the activity of the promoter 4-fold in DNA transfection experiments. This suggests that this element is a negative regulator of transcription. The factor which binds to the -339 to -361 sequence is present in type I collagen-producing cell lines but not in a variety of other cell types, suggesting that it is involved in the tissue-specific regulation of the alpha 1(I) gene.
Ravazzolo
R
R
Department of Molecular Genetics, University of Texas, M. D. Anderson Cancer Center, Houston 77030.
Karsenty
G
G
de Crombrugghe
B
B
D000818:Animals; D001685:Biological Factors / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D003094:Collagen / Q000235:genetics*; D004247:DNA / Q000235:genetics; D016172:DNA Fingerprinting; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid; D013379:Substrate Specificity; D014158:Transcription, Genetic; D014162:Transfection
D001685:Biological Factors; D003094:Collagen; D004247:DNA