Isolation and characterization of irradiation fusion hybrids from mouse chromosome 1 for mapping Rmc-1, a gene encoding a cellular receptor for MCF class murine retroviruses.
An irradiation-reduced somatic cell hybrid mapping panel was constructed of BALB/c mouse Chromosome 1. Nineteen hybrids were selected from a pool of 292 clones to generate a fine structure physical map of the distal 40 cM of the chromosome. The hybrids contain mouse DNA fragments only from Chromosome 1, ranging from approximately 5 cM to approximately 20 cM. Utilizing a viral infectibility assay, a cellular receptor gene, Rmc-1, for the MCF class of murine retroviruses was found to be linked to Lamb2, in the region between the Lamb2 and Bxv-1 loci. In addition, analysis of the hybrid mapping panel resulted in the remapping of three loci, Atpb, Ly-5, and Pmv-24, as compared to the mouse linkage map. Two previously unmapped endogenous proviruses are also putatively assigned positions on the chromosome.
Hunter
K
K
Department of Biology, Massachusetts Institute of Technology, Cambridge 01239.
Housman
D
D
Hopkins
N
N
D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D002874:Chromosome Mapping; D002875:Chromosomes / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D004279:DNA, Viral / Q000032:analysis; D008040:Genetic Linkage; D006822:Hybrid Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D012190:Retroviridae / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity
D004279:DNA, Viral