Liposomes as carriers for allergy immunotherapy.
With the aim of obtaining an efficient but safer vaccine for allergy immunotherapy, the possibility of using liposomes as adjuvants has been considered given their proven low toxicity, adjuvant properties and ability to maintain the encapsulated substance in their interior for some time in vivo. Different methods of encapsulating allergenic extracts into neutral, positively, and negatively charged DPPC: cholesterol liposomes have been investigated and the immune response provoked by these in mice was studied and compared to the immune response to free allergen or other adjuvants such as aluminium hydroxide. The results obtained show that allergen encapsulated in all three types of liposomes elicit an increase in specific IgG levels higher than that provoked by free allergen, however, both encapsulation efficiency and specific IgG titre were higher when positively charged (DPPC: cholesterol stearylamine) liposomes were used. Specific IgE levels to allergen in positive and neutral liposomes was lower than to allergen in negative liposomes or adsorbed to Al(OH)3. No difference were found in the behaviour of liposomes prepared by different methods (the results were obtained from pooled sera from different groups of mice so there is no statistical data). These results confirm the immunoadjuvant effect of liposomes for allergy immunotherapy. Further studies to determine their lack of toxicity, pharmacokinetic studies and human clinical studies are necessary to confirm their adequacy for human use and advantage over current immunotherapy methods.
Audera
C
C
Alergia e Inmunología, Abelló S.A., Madrid, Spain.
Ramírez
J
J
Soler
E
E
Carreira
J
J
D000485:Allergens / Q000008:administration & dosage* / Q000276:immunology; D000818:Animals; D003888:Desensitization, Immunologic / Q000379:methods*; D004337:Drug Carriers; D006967:Hypersensitivity / Q000628:therapy*; D007073:Immunoglobulin E / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D008081:Liposomes / Q000008:administration & dosage; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
D000485:Allergens; D004337:Drug Carriers; D007074:Immunoglobulin G; D008081:Liposomes; D007073:Immunoglobulin E