[Preservation of Claviceps purpurea, the producer of peptide ergot alkaloids, by the method of freeze drying].
A saprotrophic strain of Claviceps purpurea VNIIA 312A, an organism producing peptide +ergot alkaloids with prolactin inhibiting activity was shown to die under lyophilization conditions. To provide long-term storage of strain 312A, L-drying or drying under vacuum from liquid state was used with success. Three protective media were tested. Favourable results were obtained by using 25 per cent maltose solution as a protective medium. Preservation of the culture viability was accompanied by maintenance of the culture capacity for active formation of the biomass and production of +ergot alkaloids.
Ustiuzhanina
S V
SV
Sarkisova
M A
MA
Gorin
S E
SE
D002967:Claviceps / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D003470:Culture Media; D004876:Ergot Alkaloids / Q000378:metabolism*; D005612:Freeze Drying / Q000379:methods*; D066298:In Vitro Techniques; D007218:Industrial Microbiology / Q000379:methods*; D008320:Maltose / Q000494:pharmacology; D013997:Time Factors; D014022:Tissue Survival / Q000187:drug effects / Q000502:physiology
D003470:Culture Media; D004876:Ergot Alkaloids; D008320:Maltose