Rapid determination of the rate constant of agonist-induced association of single platelets.
We have developed a rapid and convenient method for determining the rate constant of association of single platelets. Using the concentration of single platelets prior to activation and that 0.25 min (15 s) after activation, this rate constant can be determined accurately without concern for reversibility of the aggregation process or need for statistical analysis. A linear relationship between initial platelet concentration and the rate constant of association is defined. The clinical utility of this association parameter is discussed.
Hause
L L
LL
Department of Pathology, Medical College of Wisconsin, Milwaukee 53226.
Retzinger
G S
GS
Megan
P A
PA
D000244:Adenosine Diphosphate / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D004837:Epinephrine / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D010974:Platelet Aggregation / Q000187:drug effects / Q000502:physiology*
D000244:Adenosine Diphosphate; D004837:Epinephrine