Human retinoblastoma Y79 cells contain both insulin-specific mRNA and insulin-binding sites.
We previously reported the presence of insulin-like immunoreactivity in cells from the human retinoblastoma Y79 cell line. In the present study, in situ DNA hybridization techniques were applied, using a human insulin cDNA probe to investigate whether the insulin-like activity is due to local synthesis of insulin. Our results suggest that Y79 cells contain mRNA for the synthesis of insulin or a homologous peptide. In addition, 125I-insulin binding autoradiographic studies show that these cells also contain specific insulin-binding sites. It is suggested that insulin may play an autocrine and/or paracrine role in the maintenance and metabolism of the Y79 retinoblastoma cells.
Das
A
A
Kresge Eye Institute, Wayne State University, School of Medicine, Detroit, MI 48201.
Pansky
B
B
Budd
G C
GC
Reid
T W
TW
D001345:Autoradiography; D005134:Eye Neoplasms / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D007457:Iodine Radioisotopes; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*; D011972:Receptor, Insulin / Q000378:metabolism*; D012160:Retina; D012175:Retinoblastoma / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism*
D007328:Insulin; D007457:Iodine Radioisotopes; D012333:RNA, Messenger; D011972:Receptor, Insulin