title: RecA-dependent incision of psoralen-crosslinked DNA by (A)BC excinuclease.
abstract: Previous work to elucidate the mechanism of crosslink repair by (A)BC excinuclease has shown that a psoralen-crosslinked duplex is selectively incised in the furan-side strand, while a three-stranded structure is incised in the pyrone-side strand of the crosslink. These observations support a sequential incision and recombination model for the complete error-free repair of a psoralen crosslink. The work presented here extends these findings by demonstrating that in the presence of RecA protein and a homologous DNA oligonucleotide, (A)BC excinuclease is induced to incise the pyrone-side strand of a crosslinked double-stranded plasmid molecule. This finding adds further support to the current model for error-free crosslink repair.
authors.affiliation: Department of Chemistry, University of California, Berkeley 94720.
authors.forename: S
authors.initials: S
authors.lastname: Cheng
authors.forename: A
authors.initials: A
authors.lastname: Sancar
authors.forename: J E
authors.initials: JE
authors.lastname: Hearst
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D003090:Coliphages; D003432:Cross-Linking Reagents; D004260:DNA Repair; D004279:DNA, Viral / Q000235:genetics; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D029968:Escherichia coli Proteins; D011564:Furocoumarins; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D011938:Rec A Recombinases / Q000378:metabolism*
chemical_list: D003432:Cross-Linking Reagents; D004279:DNA, Viral; D029968:Escherichia coli Proteins; D011564:Furocoumarins; D011938:Rec A Recombinases; D004706:Endodeoxyribonucleases; C036385:endodeoxyribonuclease uvrABC