Neuronal and glial marker proteins in the evaluation of the protective action of MK 801.
A quantitative dot immunobinding procedure was used to quantify glial [the S-100 protein and the glial fibrillary acidic (GFA) protein] and neuronal (the 68- and 200-kDa neurofilament polypeptides, neuron-specific enolase, and neuronal cell adhesion molecule) markers. A single intraperitoneal administration of 10 mg/kg of MK 801 blocked the increase of glial parameters and the decrease in content of neuronal marker proteins that occurred as the response to an N-methyl-D-aspartate (NMDA) lesion in the rat hippocampus. The degradation products of GFA protein and the 68-kDa neurofilament polypeptide that were induced by the NMDA lesion did not appear after MK 801 treatment. This study shows that brain-specific proteins are a set of precise tools for the evaluation of neuroprotective effects of antagonists to excitatory amino acids.
Haglid
K G
KG
Institute of Neurobiology, University of Göteborg, Sweden.
Wang
S
S
Hamberger
A
A
Lehmann
A
A
Moller
C J
CJ
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D015415:Biomarkers; D001835:Body Weight / Q000187:drug effects; D001927:Brain Diseases / Q000517:prevention & control*; D016291:Dizocilpine Maleate / Q000494:pharmacology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D015151:Immunoblotting; D007381:Intermediate Filament Proteins / Q000378:metabolism; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D015415:Biomarkers; D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; D016291:Dizocilpine Maleate