Endotoxin-induced exposure of pulmonary glycoproteins in an intact animal.
A method has been developed to radioiodinate luminally disposed endothelial proteins in an in situ perfused lung system without causing obvious vascular changes. The spectrum of endothelial cell proteins labeled in control animals and those treated with endotoxin for 45 min were compared. No changes in gross tissue morphology or in the distribution of radiolabel (125I-s-SHPP) were detected in control or treated lungs. Lectin affinity purification was applied to a lung membrane fraction to isolate labeled proteins, which were in turn resolved by gel electrophoresis and autoradiography. Comparisons of gel autoradiographs from control and treated lungs identified eight glycoproteins, the labeling of which was enhanced in endotoxin-treated animals. A similar lectin affinity analysis of radiolabeled effluent blood cells from the lungs identified only two proteins, neither of which were consistently changed by endotoxin pretreatment. A glycoprotein response can, therefore, be measured at the pulmonary endothelial surface on endotoxin administration to the whole animal without causing obvious lung injury.
Carley
W W
WW
Department of Anesthesiology, Yale University School of Medicine, New Haven, Connecticut 06510.
Merker
M
M
Gillis
C N
CN
D000818:Animals; D001345:Autoradiography; D002846:Chromatography, Affinity; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D004731:Endotoxins / Q000494:pharmacology*; D006493:Heparin / Q000031:analogs & derivatives; D007457:Iodine Radioisotopes; D008168:Lung / Q000187:drug effects / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D010477:Perfusion; D010666:Phenylpropionates; D011651:Pulmonary Artery; D011817:Rabbits; D012016:Reference Values; D013388:Succinimides
D004731:Endotoxins; D007457:Iodine Radioisotopes; D008562:Membrane Glycoproteins; D010666:Phenylpropionates; C020934:SHPP heparin; D013388:Succinimides; D006493:Heparin