Chromosome abnormalities, cellular DNA content, oncogene amplification and growth pattern in agar culture of human neuroblastomas.
The association of ability to grow in vitro, non-random chromosome abnormalities, DNA ploidy and oncogene amplification with prognosis may lead to a better understanding of the biology of neuroblastomas. In a pilot study fresh tumour tissue was obtained from 4 consecutive patients at the Department of Paediatric Surgery, Rigshospitalet, Denmark. Chromosome abnormalities were detected in 3 out of 3 successfully karyotyped tumours, and one or more aneuploid stem lines were detected in 4 out of 4 tumours using flow cytometry. The N-myc oncogene was amplified in 1 out of 3 tumours tested. Continuous cell lines could be established from all 3 advanced stage tumours. This pilot study has shown that fresh tumour tissue can be obtained and successfully studied for various fundamental aspects of the biology of neuroblastomas.
Carlsen
N L
NL
Department of Internal Medicine and Haematology, Finsen Institute, Copenhagen, Denmark.
Mortensen
B T
BT
Andersson
P K
PK
Larsen
J K
JK
Nielsen
O H
OH
Tommerup
N
N
D000362:Agar; D002455:Cell Division; D002648:Child; D002675:Child, Preschool; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D046508:Culture Techniques / Q000379:methods; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005784:Gene Amplification; D016259:Genes, myc; D011905:Genes, ras; D006801:Humans; D007223:Infant; D007621:Karyotyping; D008297:Male; D009367:Neoplasm Staging; D009447:Neuroblastoma / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D009857:Oncogenes; D014407:Tumor Cells, Cultured / Q000166:cytology
D004273:DNA, Neoplasm; D000362:Agar