Isolation and nucleotide sequence of the Aspergillus restrictus gene coding for the ribonucleolytic toxin restrictocin and its expression in Aspergillus nidulans: the leader sequence protects producing strains from suicide.
We describe the cloning and characterization of the gene coding for the ribotoxin restrictocin, from Aspergillus restrictus (gene res, EMBL accession Number X56176). This toxin is a potent inhibitor of protein synthesis in eucaryotes and is of potential interest as a component of immunotoxins. To analyze the mechanism of self-protection in the producing organism, the res gene was cloned into the vector pFB39 and introduced into Aspergillus nidulans. The secretion of active restrictocin from transformants suggests that the pro-toxin is not an active nuclease but is activated during the process of secretion.
Lamy
B
B
Unite de genie microbiologique, Institut Pasteur, Paris, France.
Davies
J
J
D000485:Allergens; D000595:Amino Acid Sequence; D000818:Animals; D052179:Antigens, Plant; D001230:Aspergillus / Q000235:genetics*; D001233:Aspergillus nidulans / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004271:DNA, Fungal; D005656:Fungal Proteins / Q000235:genetics*; D015870:Gene Expression; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D011500:Protein Synthesis Inhibitors; D011817:Rabbits; D012045:Regulatory Sequences, Nucleic Acid; D012260:Ribonucleases; D014170:Transformation, Genetic
C081947:ASPF1 protein, Aspergillus fumigatus; D000485:Allergens; D052179:Antigens, Plant; D004271:DNA, Fungal; D005656:Fungal Proteins; D011500:Protein Synthesis Inhibitors; C036196:MITF protein, Aspergillus restrictus; D012260:Ribonucleases