title: Subcellular localization and nucleosome specificity of yeast histone acetyltransferases.
abstract: We have previously reported [López-Rodas et al. (1989) J. Biol. Chem. 264, 19028-19033] that the yeast Saccharomyces cerevisiae contains four histone acetyltransferases, which can be resolved by ion-exchange chromatography, and their specificity toward yeast free histones was studied. In the present contribution we show that three of the enzymes are nuclear, type A histone acetyltransferases and they are able to acetylate nucleosome-bound histones. They differ in their histone specificity. Enzyme A1 acetylates H2A in chicken nucleosomes, although it is specific for yeast free H2B; histone acetyltransferase A2 is highly specific for H3, and histone acetyltransferase A3 preparations acetylate both H3 and H4 in nucleosomes. The fourth enzyme, which is located in the cytoplasm, does not accept nucleosomes as substrate, and it represents a canonical type B, H4-specific histone acetyltransferase. Finally, histone deacetylase activity is preferentially found in the nucleus.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Valencia, Burjassot, Spain.
authors.forename: G
authors.initials: G
authors.lastname: López-Rodas
authors.forename: V
authors.initials: V
authors.lastname: Tordera
authors.forename: M M
authors.initials: MM
authors.lastname: Sánchez del Pino
authors.forename: L
authors.initials: L
authors.lastname: Franco
mesh_terms: D000123:Acetyltransferases / Q000378:metabolism*; D000581:Amidohydrolases / Q000378:metabolism; D002467:Cell Nucleus / Q000201:enzymology; D002852:Chromatography, Ion Exchange; D051548:Histone Acetyltransferases; D006657:Histones / Q000378:metabolism; D009707:Nucleosomes / Q000201:enzymology*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins; D013379:Substrate Specificity
chemical_list: D006657:Histones; D009707:Nucleosomes; D029701:Saccharomyces cerevisiae Proteins; D000123:Acetyltransferases; D051548:Histone Acetyltransferases; D000581:Amidohydrolases