Identification of the 2A10 antigen of retinal pigment epithelium as a beta 1 subunit of integrin.
In previous studies the 2A10 antigen of retinal pigment epithelium (RPE) was described. It is a cell-surface monoclonal antibody-defined antigen whose properties suggest a possible role in cell adhesive interactions. This report identified the 2A10 antigen as a beta 1 subunit of the cell-surface extracellular matrix receptor protein, integrin. Comparative biochemical, immunochemical, and histochemical analyses of the 2A10 antibody and other antibodies directed against the beta 1 subunit of integrin indicate similar tissue distributions, immunoblotting patterns, tryptic digestion profiles, and coimmunoprecipitation of the 2A10 antigen and the beta 1 subunit of integrin. The 2A10 antigen/beta 1 subunit differed in molecular weight in different tissues, with the RPE demonstrating a form intermediate between neural and endoderm-derived tissues. The application of this new antibody to functional studies of the role of integrin in RPE cell-substrate adhesion is described in the accompanying paper.
Chu
P
P
Department of Anatomy, Jefferson Medical College of Thomas Jefferson University, Philadelphia, PA 19107.
Grunwald
G B
GB
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D016326:Extracellular Matrix Proteins / Q000276:immunology*; D005136:Eye Proteins / Q000276:immunology*; D005347:Fibroblasts / Q000276:immunology; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D016023:Integrins / Q000276:immunology*; D010857:Pigment Epithelium of Eye / Q000276:immunology*; D011233:Precipitin Tests
D000954:Antigens, Surface; D016326:Extracellular Matrix Proteins; D005136:Eye Proteins; D016023:Integrins