Light-induced, long-lived perturbation of the photocycle of bacteriorhodopsin.
The relative weight of the slowly decaying M intermediate of the photocycle of bacteriorhodopsin increases upon increasing the energy density of the short (10 ns) actinic laser pulse. Moreover, when a pre-exciting flash is applied to the BR sample, the absolute amplitude of the Ms is higher in the signal induced by a second flash, applied with a delay from 100 microseconds to 100 ms. These facts together prove that either the leftover BR ground-state population becomes different due to the pre-excitation, or there is a cooperative interaction between the BR molecules.
Tokaji
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Institute of Biophysics, Biology Research Centre, Szeged, Hungary.
Dancsházy
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D001436:Bacteriorhodopsins / Q000378:metabolism* / Q000528:radiation effects; D007700:Kinetics; D008027:Light; D013997:Time Factors
D001436:Bacteriorhodopsins