Oct-3 is a maternal factor required for the first mouse embryonic division.
Oct-3 is a POU domain transcription factor that binds the octamer DNA motif and is present in mouse oocytes before and after fertilization. When fertilized oocytes were injected with antisense Oct-3 oligonucleotides or double-stranded DNA containing the octamer motif, embryonic DNA synthesis was inhibited and the embryos were arrested at the one-cell stage. In vitro synthesized Oct-3 mRNA rescued the developmental block induced by antisense Oct-3 oligonucleotide. We conclude that maternally inherited Oct-3 is required for DNA replication and division of the one-cell embryo.
Rosner
M H
MH
Metabolism Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
De Santo
R J
RJ
Arnheiter
H
H
Staudt
L M
LM
D000818:Animals; D001483:Base Sequence; D002455:Cell Division / Q000502:physiology; D002970:Cleavage Stage, Ovum / Q000737:chemistry* / Q000502:physiology; D004261:DNA Replication / Q000502:physiology; D004268:DNA-Binding Proteins / Q000502:physiology*; D005314:Embryonic and Fetal Development / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D050814:Octamer Transcription Factor-3; D016376:Oligonucleotides, Antisense; D009865:Oocytes / Q000737:chemistry*; D012333:RNA, Messenger / Q000378:metabolism; D014157:Transcription Factors / Q000502:physiology*; D015053:Zygote / Q000737:chemistry* / Q000502:physiology
D004268:DNA-Binding Proteins; D050814:Octamer Transcription Factor-3; D016376:Oligonucleotides, Antisense; C494174:Pou5f1 protein, mouse; D012333:RNA, Messenger; D014157:Transcription Factors