Effect of mutations affecting the p6 gag protein on human immunodeficiency virus particle release.
Mutations in sequences at the C terminus of the capsid precursor protein of human immunodeficiency virus type 1 that affect the viral p6 protein prevent release of budded virus particles from the cell surface. The experiments reported here define an important step in the life cycle of the virus, the release of the budded particle from a tether that binds the assembled particle to the cell surface. Inhibition of the release of the viral capsid proteins by interferon alpha indicates that this step of virus maturation may be sensitive to inhibition by antiviral drugs.
Göttlinger
H G
HG
Dana-Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, MA 02115.
Dorfman
T
T
Sodroski
J G
JG
Haseltine
W A
WA
D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D002462:Cell Membrane / Q000648:ultrastructure; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004252:DNA Mutational Analysis; D015683:Gene Products, gag / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D054298:Human Immunodeficiency Virus Proteins; D007370:Interferon Type I / Q000494:pharmacology; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D011498:Protein Precursors / Q000378:metabolism; D054334:Viral Regulatory and Accessory Proteins / Q000502:physiology; D014779:Virus Replication; D000086082:Viroporin Proteins
D015683:Gene Products, gag; D054298:Human Immunodeficiency Virus Proteins; D007370:Interferon Type I; D009841:Oligonucleotides; D011498:Protein Precursors; D054334:Viral Regulatory and Accessory Proteins; C514859:vpu protein, Human immunodeficiency virus 1; D000086082:Viroporin Proteins