Validity of flow cytometry for cross-match evaluation in clinical renal transplantation.
This paper reports a 2-year experience of more than 5,000 cross-match tests for renal transplantation. Tests were performed by means of both standard light microscopy and an innovatory method based on flow cytometry, an up-to-date investigative technique for computerized analysis of individual cell characteristics. Flow cytometry allowed a better detection of weak positive reactions (false-negative cross-matches) than light microscopy, thus reducing the risk of selecting candidates with donor presensitization. Transplant clinical outcome supported the value of this original and advanced technological method.
Stefoni
S
S
Institute of Nephrology, University of Bologna, Italy.
Nanni-Costa
A
A
Buscaroli
A
A
Borgnino
L C
LC
Iannelli
S
S
Raimondi
C
C
Scolari
M P
MP
Feliciangeli
G
G
Bonomini
V
V
D000906:Antibodies / Q000032:analysis; D005069:Evaluation Studies as Topic; D005434:Flow Cytometry / Q000379:methods*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology
D000906:Antibodies