Construction and characterization of a partial library of yeast artificial chromosomes from human chromosome 21.
We report a protocol for cloning large DNA fragments in yeast artificial chromosomes (YAC). A partial library has been constructed from a somatic hybrid containing chromosome 21 as the single source of human DNA. About 4.0 Mb of human DNA was recovered in 17 YAC clones. Three clones were analyzed by in situ hybridization and mapped on chromosome 21. One clone hybridized with the chromosome 21 centromeric region and may provide new insight both on the molecular structure of centromere and on the localization of Alzheimer disease gene.
Bellis
M
M
CNRS UPR 8402 & INSERM U. 249, Institut de Biologie, Montpellier, France.
Gérard
A
A
Charlieu
J P
JP
Marçais
B
B
Brun
M E
ME
Viegas-Péquignot
E
E
Carter
D A
DA
Roizès
G
G
D001483:Base Sequence; D001710:Biotin; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D002891:Chromosomes, Human, Pair 21; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D015723:Gene Library; D015894:Genome, Human; D006801:Humans; D007621:Karyotyping; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction
D001710:Biotin; D004247:DNA