Evaluation of two methods for overcoming the antibiotic carry-over effect.
The antibiotic carry-over effect occurs when antibiotic transferred onto the agar plate with the subcultured aliquot is sufficient to inhibit the growth of viable bacteria, and results in a falsely low MBC. This phenomenon was eliminated by widely streaking the transferred aliquot over at least one half of a 100 mm agar plate or by centrifugation and resuspension of the organisms in non-antibiotic-containing media prior to plating. Both methods require more effort than the current method of MBC determination, but can be performed in clinical laboratories and are thus highly recommended in the testing of organisms from endocarditis and meningitis patients.
Eng
R H
RH
Infectious Disease Section/Medical Service, Veterans Administration Medical Center, East Orange, New Jersey 07019.
Smith
S M
SM
Cherubin
C E
CE
Tan
E N
EN
D002498:Centrifugation; D015169:Colony Count, Microbial / Q000379:methods*; D005069:Evaluation Studies as Topic; D008826:Microbial Sensitivity Tests / Q000379:methods*