Identification of the site of autophosphorylation of the bacterial protein kinase/phosphatase NRII.
Previous studies have established that the Escherichia coli protein kinase/phosphatase nitrogen regulator II (NRII also known as NtrB) becomes autophosphorylated on a histidine residue when incubated with ATP. We show that the major site at which NRII was autophosphorylated was contained within a peptide consisting of amino acid residues 136-142 of NRII, and thus probably corresponds to His-139. A minor site of phosphorylation, accounting for about 2% of the phosphate in NRII-P, was found in a peptide that corresponds to residues 158-169.
Ninfa
A J
AJ
Department of Biochemistry, Wayne State University School of Medicine, Detroit, Michigan 48201.
Bennett
R L
RL
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D008969:Molecular Sequence Data; D051922:PII Nitrogen Regulatory Proteins; D010446:Peptide Fragments / Q000302:isolation & purification; D010748:Phosphopeptides / Q000302:isolation & purification; D010749:Phosphoprotein Phosphatases; D010766:Phosphorylation; D011494:Protein Kinases; D015534:Trans-Activators; D014157:Transcription Factors; D014357:Trypsin
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D051922:PII Nitrogen Regulatory Proteins; D010446:Peptide Fragments; D010748:Phosphopeptides; D015534:Trans-Activators; D014157:Transcription Factors; D011494:Protein Kinases; C055699:protein kinase-phosphatase NTRB; D010749:Phosphoprotein Phosphatases; D014357:Trypsin