Two cis-acting regulatory sequences in the peroxisome proliferator-responsive enhancer region of rat acyl-CoA oxidase gene.
To clarify the mechanism of transcriptional induction of the rat liver acyl-CoA oxidase gene by hypolipidemic agents, we searched for cis-acting regulatory sequences in the 5' upstream region of the gene by transfection studies. We found that the sequence between -639 and -472 acts as a peroxisome proliferator-responsive, tissue-specific enhancer. Footprint analysis revealed two protein binding sites in this region. One of these sequences exhibited a positive, whereas the other a negative, regulatory activity in transcription assays.
Osumi
T
T
Department of Biochemistry, Shinshu University School of Medicine, Matsumoto, Nagano, Japan.
Wen
J K
JK
Hashimoto
T
T
D042962:Acyl-CoA Oxidase; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003850:Deoxyribonuclease I; D004742:Enhancer Elements, Genetic; D015971:Gene Expression Regulation, Enzymologic; D008099:Liver / Q000201:enzymology*; D008114:Liver Neoplasms, Experimental; D008830:Microbodies / Q000502:physiology*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics*; D011401:Promoter Regions, Genetic; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014162:Transfection
D010088:Oxidoreductases; D042962:Acyl-CoA Oxidase; D003850:Deoxyribonuclease I