title: Expression of the rat aldolase B gene: a liver-specific proximal promoter and an intronic activator.
abstract: The nature and location of the cis-acting DNA sequences regulating expression of the rat aldolase B gene has been investigated. Two liver-specific DNAse I hypersensitive sites were detected, one located just upstream from the cap site, the second in the middle of the first, 4.8-kbp-long, intron. A fragment of 190 bp 5' to the cap site behaved as a tissue-specific but weak core promoter: it directed a detectable reporter gene expression in the Hep G2 cells and hepatocytes, but not in fibroblasts. The tissue-specific expression was stimulated at least 16 fold when constructs contained the entire first intron. The intronic activating sequences could be ascribed to an inner 2 kbp fragment in which the downstream liver-specific DNAse I hypersensitive site was located.
authors.affiliation: ICGM, INSERM U. 129 (Laboratoire de Recherches en Génétique et Pathologie Moléculaires), CHU Cochin, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Gregori
authors.forename: F
authors.initials: F
authors.lastname: Ginot
authors.forename: J F
authors.initials: JF
authors.lastname: Decaux
authors.forename: A
authors.initials: A
authors.lastname: Weber
authors.forename: T
authors.initials: T
authors.lastname: Berbar
authors.forename: A
authors.initials: A
authors.lastname: Kahn
authors.forename: A L
authors.initials: AL
authors.lastname: Pichard
mesh_terms: D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics; D016384:Consensus Sequence; D003850:Deoxyribonuclease I; D005347:Fibroblasts / Q000201:enzymology; D005634:Fructose-Bisphosphate Aldolase / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D007438:Introns; D008099:Liver / Q000201:enzymology*; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D012680:Sensitivity and Specificity; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D015500:Chloramphenicol O-Acetyltransferase; D003850:Deoxyribonuclease I; D005634:Fructose-Bisphosphate Aldolase