Organization and chromosomal localization of the human platelet-derived endothelial cell growth factor gene.
Human platelet-derived endothelial cell growth factor (hPD-ECGF) is a novel angiogenic factor which stimulates endothelial cell growth in vitro and promotes angiogenesis in vivo. We report here the cloning and sequencing of the gene for hPD-ECGF and its flanking regions. This gene is composed of 10 exons dispersed over a 4.3-kb region. Its promoter lacks a TATA box and a CCAAT box, structures characteristic of eukaryotic promoters. Instead, six copies of potential Sp1-binding sites (GGGCGG or CCGCCC) were clustered just upstream of the transcription start sites. Southern blot analysis using genomic DNAs from several vertebrates suggested that the gene for PD-ECGF is conserved phylogenetically among vertebrates. The gene for hPD-ECGF was localized to chromosome 22 by analysis of a panel of human-rodent somatic cell hybrid lines.
Hagiwara
K
K
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Stenman
G
G
Honda
H
H
Sahlin
P
P
Andersson
A
A
Miyazono
K
K
Heldin
C H
CH
Ishikawa
F
F
Takaku
F
F
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22; D003001:Cloning, Molecular; D004730:Endothelium, Vascular / Q000166:cytology*; D005091:Exons; D005260:Female; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D013939:Thymidine Phosphorylase / Q000235:genetics*; D014158:Transcription, Genetic
D013939:Thymidine Phosphorylase