Actinomycin D induced DNase I hypersensitivity and asymmetric structure transmission in a DNA hexadecamer.
DNase I cleavage rates and nmr chemical shifts are shown to change for DNA sequences distal to an intercalated actinomycin D molecule in a duplex hexadecamer upon drug binding. Both sets of observations suggest that the source of these changes is a DNA-mediated structural response. The nmr results imply the response is transmitted preferentially in a 5'-to-3' direction from the drug binding site. An inequivalent response of the two strands to a ligand-induced conformational change immediately suggests a mechanism for distinguishing the sense and antisense strands of DNA.
Bishop
K D
KD
Department of Chemistry, Syracuse University, NY 13244-4100.
Borer
P N
PN
Huang
Y Q
YQ
Lane
M J
MJ
D001483:Base Sequence; D003609:Dactinomycin / Q000378:metabolism / Q000494:pharmacology*; D003850:Deoxyribonuclease I / Q000737:chemistry*; D007364:Intercalating Agents; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes
D007364:Intercalating Agents; D009692:Nucleic Acid Heteroduplexes; D003609:Dactinomycin; D003850:Deoxyribonuclease I