[Molecular epidemiological markers of Legionella pneumophila in the Valencian Community (III). Peptide profiles].
Electrophoretic profiles using soluble peptides obtained from Legionella pneumophila serogroup 1 have been studied to be used as epidemiological markers. Using 8.5% polyacrylamide gels (SDS-PAGE) a unique profile common to environmental and clinical isolates has been detected showing 2 differential bands: a 150 kD and a 230 kD band present in only one clinical isolate. In order to achieve better resolution in the peptide profile 10 to 20% gradient SDS-PAGE has been used, confirming the existence of a common profile and 2 subtypes as well as another profile present in a smaller number of strains.
Muñoz
C
C
Departamento de Microbiología, Facultad de Medicina, Hospital Clínico Universitario, Valencia.
Catalán
V
V
Borrás
R
R
García-de-Lomas
J
J
D001426:Bacterial Proteins / Q000032:analysis*; D015415:Biomarkers; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D007875:Legionella / Q000032:analysis / Q000145:classification*; D007877:Legionnaires' Disease / Q000453:epidemiology / Q000382:microbiology*; D010455:Peptides / Q000302:isolation & purification*; D013030:Spain / Q000453:epidemiology
D001426:Bacterial Proteins; D015415:Biomarkers; D010455:Peptides