Direct measurement of high-density lipoprotein cholesterol by the reflotron assay with no manual precipitation step.
We evaluated the quantification of high-density lipoprotein (HDL) cholesterol in plasma with the Boehringer Mannheim Reflotron reflectance photometric analyzer. The Reflotron is designed for testing in small to medium-size laboratories and physicians' offices. This HDL method does not require a manual precipitation step because the reagent tab contains dextran sulfate (Mr 50,000) and magnesium acetate. It takes 90 s to complete an analysis of 30 microL of plasma. Within-day standard deviations (SDs) were 0.02-0.04 mmol/L (6-16 mg/L). Total SDs over a three-month period were 0.03-0.06 mmol/L (11-23 mg/L). The Reflotron values averaged 0.02 mmol/L (6 mg/L) or 1.3% lower than the Hitachi 737 values; the standard error of the estimate (Sy.x) was 0.07 mmol/L (29 mg/L).
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Department of Pathology & Laboratory Medicine, Methodist Hospital of Indiana, Indianapolis 46202.
Sparks
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KM
Statland
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BE
D001205:Ascorbic Acid / Q000494:pharmacology; D001663:Bilirubin / Q000494:pharmacology; D008076:Cholesterol, HDL / Q000097:blood*; D006454:Hemoglobins; D006801:Humans; D010783:Photometry / Q000379:methods*; D011933:Reagent Kits, Diagnostic
D008076:Cholesterol, HDL; D006454:Hemoglobins; D011933:Reagent Kits, Diagnostic; D001205:Ascorbic Acid; D001663:Bilirubin