title: Effects of adjuvant-augmented germling vaccines in turkey poults challenged with Aspergillus fumigatus.
abstract: Turkey poults were vaccinated with combinations of two different germling preparations and three adjuvants (N-acetylmuranyl-L-alanyl-D-isoglutamine, Pasteurella multocida lipopolysaccharide [LPS], and avridine) at 1 and 2 weeks of age, and their immunity was challenged by sublethal exposure to aerosols of Aspergillus fumigatus conidia at 1 month of age. Fewer turkeys in the groups given vaccines prepared from germlings grown on Dorset's and Henley's medium (D&H) had organisms in lung tissue at 2 weeks after challenge exposure as compared with those vaccinated with germling grown on neopeptone dialysate (Neo). The LPS of P. multocida appeared to be the most efficacious of the adjuvants in the D&H vaccine group, as A. fumigatus was isolated from only one of eight turkeys in this group; the number of organisms per gram of lung tissue was low compared with other vaccine groups at 2 weeks after challenge exposure; and poults given D&H vaccine with LPS as adjuvant had less-severe lung lesions than other groups. These differences in lung lesions were more marked at 2 weeks than at 8 weeks after challenge exposure. The only difference among other parameters in the vaccinated turkeys was lower heterophil counts in the turkeys given D&H-prepared vaccines than in unvaccinated controls. This was probably due to less-severe infections resulting from protective effects of these vaccines.
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, Ames, Iowa 50010.
authors.forename: J L
authors.initials: JL
authors.lastname: Richard
authors.forename: W M
authors.initials: WM
authors.lastname: Peden
authors.forename: J M
authors.initials: JM
authors.lastname: Sacks
mesh_terms: D000276:Adjuvants, Immunologic; D000400:Air Sacs / Q000473:pathology; D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood; D001228:Aspergillosis / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D001232:Aspergillus fumigatus / Q000276:immunology*; D005260:Female; D005657:Fungal Vaccines; D006098:Granulocytes; D006099:Granuloma / Q000473:pathology / Q000662:veterinary; D007958:Leukocyte Count / Q000662:veterinary; D008168:Lung / Q000473:pathology; D008172:Lung Diseases, Fungal / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary; D008214:Lymphocytes; D008297:Male; D011201:Poultry Diseases / Q000473:pathology / Q000517:prevention & control*; D014422:Turkeys; D015430:Weight Gain
chemical_list: D000276:Adjuvants, Immunologic; D000908:Antibodies, Fungal; D005657:Fungal Vaccines