Assay for nucleoside and nucleotide binding of a potent mutagen, 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone.
Deoxyadenosine, deoxyguanosine, deoxycytidine; thymidine and a dinucleotide, 2'-deoxycytidylyl-(3'-5')-2'-deoxythymidine (dCdT), were reacted with 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX). Nucleoside HPLC analysis revealed that the major products are the degradation products of MX and no stable MX-adducts were found. The same result was obtained with sodium borohydride reduction. The fluorescence spectra of deoxyguanosine-MX showed no fluorescence at neutral or alkaline pH, indicating no binding products to the N2-groups of deoxyguanosine.
Alhonen-Raatesalmi
A
A
Institute of Occupational Health, Helsinki, Finland.
Hemminki
K
K
D001894:Borohydrides / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004247:DNA / Q000378:metabolism; D003839:Deoxyadenosines / Q000378:metabolism; D003849:Deoxyguanosine / Q000378:metabolism; D003853:Deoxyribonucleosides / Q000378:metabolism; D005470:Fluorometry / Q000379:methods; D005663:Furans / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009153:Mutagens / Q000378:metabolism*; D009705:Nucleosides / Q000378:metabolism*; D009711:Nucleotides / Q000378:metabolism*; D010084:Oxidation-Reduction; D013050:Spectrometry, Fluorescence
D001894:Borohydrides; D003839:Deoxyadenosines; D003853:Deoxyribonucleosides; D005663:Furans; D009153:Mutagens; D009705:Nucleosides; D009711:Nucleotides; C054121:3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone; C025364:sodium borohydride; D004247:DNA; D003849:Deoxyguanosine