Electrolyte induced vesiculation of MA782 mice mammary adenocarcinoma ascites cells.
MA782 ascites cells were found to vesiculate when incubated in electrolyte solutions and release giant plasma membrane vesicles with diameters ranging from 5 microns to 15 microns. The vesicles, generated from the plasma membrane and free of cellular organelles, as visualized by microscopy and marker-enzyme analysis, could be proposed as a useful model membrane system.
Tan
Z
Z
Department of Cell Biology, Chinese Academy of Sciences, Beijing.
Fang
S G
SG
Liu
S S
SS
D000230:Adenocarcinoma / Q000473:pathology / Q000648:ultrastructure*; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure*; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000473:pathology / Q000648:ultrastructure*; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D015388:Organelles / Q000648:ultrastructure*