title: Interaction of the antibiotic sparsomycin with the ribosome.
abstract: Phenol-alanine-sparsomycin, a derivative of sparsomycin carrying a p-hydroxy-benzyl function easily labeled by iodination, has been used to study the interaction of this drug with the ribosome. Our study indicated that the binding of the drug to the ribosome is sensitive to trichloracetic acid and is equally affected by disintegration of the particle after RNase and protease treatments. The ribosome is not irreversibly inactivated, and the chemical structure of the drug is not affected by interaction with the particle. These data are not compatible with the proposed covalent association of sparsomycin with the ribosome by G. A. Flynn and R. J. Ash (Biochem. Biophys. Res. Commun. 114:1-7, 1983); therefore, the antibiotic must inhibit protein synthesis through a reversible interaction with the ribosome.
authors.affiliation: Centro de Biología Molecular, Universidad Autónoma, Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Lazaro
authors.forename: A
authors.initials: A
authors.lastname: San Felix
authors.forename: L A
authors.initials: LA
authors.lastname: van den Broek
authors.forename: H C
authors.initials: HC
authors.lastname: Ottenheijm
authors.forename: J P
authors.initials: JP
authors.lastname: Ballesta
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D004926:Escherichia coli / Q000378:metabolism / Q000648:ultrastructure; D010455:Peptides / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D013033:Sparsomycin / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D010455:Peptides; C068211:phenol-alanine sparsomycin; D013033:Sparsomycin