title: Cloning and characterization of gentamicin-resistance genes from Micromonospora purpurea and Micromonospora rosea.
abstract: Aminoglycoside-resistance genes (grm) were cloned from a gentamicin producer Micromonospora purpurea and a sisomicin producer Micromonospora rosea. The nucleotide (nt) sequences of both genes were determined and the similarity between them was very high (90.4% identity). In either case, the transcription start point was localised to about 11 nt upstream from the likely translation start codons of grm, which is expressed as a polycistronic transcript. In studies to be reported elsewhere, it has been established that the M. purpurea grm gene encodes a ribosomal RNA methyltransferase. Here, we confirmed that the similarity of the two genes exists not only at the structural but also at the functional level.
authors.affiliation: Department of Biochemistry, University of Leicester, U.K.
authors.forename: G H
authors.initials: GH
authors.lastname: Kelemen
authors.forename: E
authors.initials: E
authors.lastname: Cundliffe
authors.forename: I
authors.initials: I
authors.lastname: Financsek
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial; D005839:Gentamicins / Q000494:pharmacology*; D008847:Micromonospora / Q000235:genetics*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D004269:DNA, Bacterial; D005839:Gentamicins