Effects of heparin treatment on colonic torsion-associated hemodynamic and plasma eicosanoid changes in anesthetized ponies.
Large colon torsion frequently is a fatal condition in horses. The purpose of the study reported here was to determine systemic arterial pressure, plasma eicosanoid concentrations, colonic blood flow, vascular resistance, tissue pH, and morphologic features associated with large colon torsion and detorsion, and to evaluate the effects of sodium heparin (80 IU/kg of body weight, IV) treatment on these values. Values were determined in 20 anesthetized ponies that were randomly assigned into 4 equal groups: control; control/heparin; torsion; torsion/heparin. Torsions were created by a 720 degrees rotation of the cecum and colon around their long axes at the sternal and diaphragmatic flexures. After 1 hour of torsion, the torsion was corrected and the colon was allowed to reperfuse for 1 hour. Heparin was administered 30 minutes into the experiment. Parametric data were analyzed (P less than or equal to 0.05), using split-plot analysis of variance, with differences between means evaluated with a modified Bonferroni t test; histopathologic data were analyzed (P less than or equal to 0.05) with a Kruskal-Wallis one-way analysis of variance by ranks. Heparin prevented colonic detorsion-induced hypotension and increases in vascular resistance and thromboxane concentration, and it significantly increased colonic blood flow for 40 minutes during reperfusion. Heparin did not alter prostacyclin concentration or the histologic appearance of the large colon.
Provost
P J
PJ
Department of Large Animal Clinical Sciences, Michigan State University, East Lansing 48824-1314.
Stick
J A
JA
Patterson
J S
JS
Hauptman
J G
JG
Robinson
N E
NE
Roth
R
R
D000758:Anesthesia / Q000662:veterinary; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D003106:Colon / Q000098:blood supply / Q000503:physiopathology; D003108:Colonic Diseases / Q000097:blood / Q000188:drug therapy / Q000503:physiopathology / Q000662:veterinary*; D011464:Epoprostenol / Q000097:blood*; D005260:Female; D006439:Hemodynamics / Q000187:drug effects*; D006493:Heparin / Q000008:administration & dosage / Q000494:pharmacology*; D006734:Horse Diseases / Q000097:blood / Q000188:drug therapy* / Q000503:physiopathology; D006736:Horses; D006863:Hydrogen-Ion Concentration; D007415:Intestinal Obstruction / Q000097:blood / Q000188:drug therapy / Q000503:physiopathology / Q000662:veterinary*; D008297:Male; D013928:Thromboxane A2 / Q000097:blood*; D014655:Vascular Resistance / Q000187:drug effects
D013928:Thromboxane A2; D006493:Heparin; D011464:Epoprostenol