Dietary fat and phospholipase A2 activity of Sprague-Dawley rat large intestine.
The present studies were conducted to examine the effect of dietary lipid content and composition [(n-6) vs. (n-3) fatty acids] on the activity of mucosal phospholipase (PL)A2 of the large intestinal tract of rats. Three segments of the large intestinal tract were examined: cecum, proximal colon and distal colon. Weanling male Sprague-Dawley rats were fed diets containing either 5% (LS) or 16% safflower (HS) or 14% menhaden oil plus 2% safflower oil (HM) for 3 wk with the oil replacing starch in the HS and HM diets on a weight basis. The lipid extracts of microsomal fractions from mucosal scrapings were examined for phospholipid and cholesterol content and fatty acid composition. Phospholipase A2 was assayed using a fluorescent substrate. Rats fed the high fat diets had lower PLA2 specific activities. The (n-3) or (n-6) fatty acid enrichment of the membranes had no effect of the activity of the enzyme. The activity of the enzyme decreased aborally from the cecum to the distal colon; the proximal colon had an intermediate specific activity.
Awad
A B
AB
Nutrition Program, State University of New York, Buffalo 14214.
Mallen
S R
SR
Horvath
P J
PJ
Fink
C S
CS
D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002432:Cecum / Q000201:enzymology; D003106:Colon / Q000201:enzymology; D004041:Dietary Fats / Q000378:metabolism*; D007420:Intestine, Large / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008861:Microsomes / Q000201:enzymology; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity
D004041:Dietary Fats; D010743:Phospholipids; D010741:Phospholipases A; D054467:Phospholipases A2