Syntheses and studies of metaphase-arresting pyrimidinones.
Methods are described for the syntheses of chloromethyl hydroxyalkylphenyl and benzyl ethers, and for the synthesis of bromomethyl phenyl ketone analogs. The hydroxy groups were protected as acetates. The halogenomethyl derivatives have been used for N-alkylation of 5-chloro-2(1H)-pyrimidinone. The acetyl groups in the products were removed by aminolysis or by enzymatic (pig liver esterase) hydrolysis. The hydroxy derivatives are chemically labile because of polymerization reactions. Adduct formation (1:1) with sodium hydrogensulfite improved the stability. The products are specific inhibitors of the cell cycle in the metaphase. In vitro data are given from screening in cultivated Chang liver cells.
Benneche
T
T
Department of Chemistry, University of Oslo, Norway.
Keilen
G
G
Hagelin
G
G
Oftebro
R
R
Undheim
K
K
D000818:Animals; D002460:Cell Line; D055598:Chemical Phenomena; D002621:Chemistry; D004126:Dimethylformamide; D006801:Humans; D006868:Hydrolysis; D007939:Leukemia L1210; D008677:Metaphase / Q000187:drug effects*; D011744:Pyrimidinones / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology
D011744:Pyrimidinones; D004126:Dimethylformamide