[Use of a heptane-isopropanol system for extraction of primary lipid peroxidation products].
Products of different lipids oxidation were studied for the peculiarities of their distribution in the extracting system heptane--isopropanol widely used in spectrophotometric determination of primary products of peroxide oxidation of lipids. It is shown that hydroperoxides of phospholipids and their reduction products almost completely transfer to the alcohol phase while hydroperoxides of triacetin and cholesterol esters are 70-85% extracted by heptane. Thus, it is necessary to analyze the alcohol phase when using the systems heptane-isopropanol to determine the content of primary products of lipid peroxide oxidation in those tissues, where phospholipids are the basic oxidation substrates.
Kostiuk
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D000433:1-Propanol; D006536:Heptanes; D008054:Lipid Peroxides / Q000302:isolation & purification*
D006536:Heptanes; D008054:Lipid Peroxides; D000433:1-Propanol