title: Major component of Ra-reactive factor, a complement-activating bactericidal protein, in mouse serum.
abstract: A complement-activating bactericidal protein, Ra-reactive factor was isolated from mouse serum by an affinity method. The m.w. of the isolated RaRF estimated by glycerol density gradient sedimentation (around 300,000) was the same as that of the active material in mouse serum. As evidenced by gel filtration, the intact RaRF was decomposed into high (higher than 200,000) and low (50,000 to 200,000) m.w. components by treatment with 10% acetonitrile. SDS- and acid/urea-PAGE demonstrated that the high m.w. component was completely dissociated into equimolar quantities of two kinds of 28 kDa polypeptides, P28a and P28b, under reducing conditions, indicating that the association of these polypeptides was stabilized by disulfide bonds. The ability to bind specifically to the Ra determinant was retained in the high m.w. component, although the complement-activating potency was lost. The amino acid compositions of P28a and P28b polypeptides were compared with those of related serum proteins. The P28a and P28b polypeptides were found to have the highest homology to rat mannan-binding protein and mouse and human C1q subcomponent of complement.
authors.affiliation: Department of Molecular Biology, School of Medicine, Kitasato University, Kanagawa, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ihara
authors.forename: A
authors.initials: A
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Hatsuse
authors.forename: K
authors.initials: K
authors.lastname: Sumitomo
authors.forename: K
authors.initials: K
authors.lastname: Doi
authors.forename: M
authors.initials: M
authors.lastname: Kawakami
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001770:Blood Bactericidal Activity / Q000276:immunology*; D001798:Blood Proteins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D003167:Complement Activation; D046911:Macromolecular Substances; D051379:Mice; D008813:Mice, Inbred ICR; D008970:Molecular Weight; D010455:Peptides / Q000302:isolation & purification
chemical_list: D000596:Amino Acids; D001798:Blood Proteins; D046911:Macromolecular Substances; D010455:Peptides