title: Freezing lesions of the developing rat brain: a model for cerebrocortical microgyria.
abstract: Cerebrocortical microgyri were induced by placing a freezing probe on the skull of P0 and P1 rat pups. Freezing lesions resulted in laminar necrosis of the infragranular layers and the subsequent migration of supragranular neurons through the region of damage. The result was most often a region of four-layered microgyric cortex consisting of a molecular layer, a thickened layer ii, a lamina dissecans (corresponding to the necrotized layers IV, V, and VIa), and a neuronal layer iv which corresponded to layer VIb of the intact cortex. Immunocytochemical investigation of the microgyric cortex with antibodies to neurofilament, glial fibrillary acidic protein and glutamate showed more widespread disruption of neocortical architecture than could be seen from Nissl preparations. In contrast, vasoactive intestinal peptide-containing neuronal bodies appeared to be distributed normally in the microgyric region although their processes were sometimes distorted. These results are considered in the light of previous research on induced microgyria, and possible implications for the behavioral consequences of focal, developmental neuropathologic lesions are discussed.
authors.affiliation: Dyslexia Research Laboratory, Beth Israel Hospital, Boston, MA 02215.
authors.forename: P
authors.initials: P
authors.lastname: Humphreys
authors.forename: G D
authors.initials: GD
authors.lastname: Rosen
authors.forename: D M
authors.initials: DM
authors.lastname: Press
authors.forename: G F
authors.initials: GF
authors.lastname: Sherman
authors.forename: A M
authors.initials: AM
authors.lastname: Galaburda
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000254:growth & development / Q000473:pathology*; D002540:Cerebral Cortex / Q000002:abnormalities* / Q000473:pathology; D004195:Disease Models, Animal; D005557:Formaldehyde; D005615:Freezing; D006652:Histological Techniques; D007150:Immunohistochemistry; D009336:Necrosis; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D005557:Formaldehyde