Use of chicken antibodies in enzyme immunoassays to avoid interference by rheumatoid factors.
Rheumatoid factor (RF) is a major source of interference in many immunoassays. Most immunoassays use mammalian polyclonal or monoclonal antibodies, and RF can react with IgG from mammalian species, thus causing false-positive results. In this work we have studied RF interference in a sandwich ELISA, where RF in the sample may react with both the capture antibody and the detection antibody to give a false-positive reaction. We show that rheumatoid factors do not react with chicken IgY; if the capture antibody or detection antibody (or both) is of avian origin, the interference of RF or other anti-IgG antibodies in sandwich ELISA can be avoided.
Larsson
A
A
Department of Medical and Physiological Chemistry, Biomedical Center, Uppsala, Sweden.
Karlsson-Parra
A
A
Sjöquist
J
J
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000302:isolation & purification*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005189:False Positive Reactions; D006801:Humans; D007106:Immune Sera; D007074:Immunoglobulin G / Q000276:immunology*; D011817:Rabbits; D012217:Rheumatoid Factor / Q000276:immunology*; D013045:Species Specificity
D000888:Antibodies, Anti-Idiotypic; D007106:Immune Sera; D007074:Immunoglobulin G; D012217:Rheumatoid Factor