title: In vitro and in vivo evaluation of effects of a heptanoyl tripeptide, FK-565, on porcine macrophage and lymphocyte function.
abstract: A series of experiments was performed in vitro and in vivo to determine the influence of FK-565, a heptanoyl tripeptide, on lymphocyte and macrophage function in swine. Compared with values for control cultures, mitogen-stimulated lymphocyte blastogenesis and interleukin-2 production were unaffected in cells preincubated with 0.1, 1.0, and 10.0 micrograms of FK-565/ml. Natural killer cell activity was increased by preincubation with 1.0 microgram of FK-565/ml; however, this increase was not statistically significant. In vitro treatment of porcine alveolar macrophages with FK-565 did not enhance cytolytic activity or bactericidal activity. In in vivo experiments, FK-565 given orally to pigs at concentrations of 6 or 60 micrograms.kg-1.d-1 for 5 days did not affect lymphocyte blastogenesis, interleukin-2 production, or alveolar macrophage bactericidal activity. A trend toward increased natural killer cell activity was evident in pigs treated with FK-565. In contrast, pigs treated with 6 micrograms.kg-1.d-1 had significantly (P less than 0.01) decreased alveolar macrophage cytolytic activity. These data indicate that at the dosages tested, FK-565 is not a suitable immunomodulator for enhancement of nonspecific immunity in swine.
authors.affiliation: Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan 66506.
authors.forename: C G
authors.initials: CG
authors.lastname: Chitko
authors.forename: S K
authors.initials: SK
authors.lastname: Chapes
authors.forename: R C
authors.initials: RC
authors.lastname: Thaler
authors.forename: J L
authors.initials: JL
authors.lastname: Nelssen
authors.forename: G A
authors.initials: GA
authors.lastname: Anderson
authors.forename: E R
authors.initials: ER
authors.lastname: Clough
authors.forename: F
authors.initials: F
authors.lastname: Blecha
mesh_terms: D000818:Animals; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D005069:Evaluation Studies as Topic; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000502:physiology; D008264:Macrophages / Q000187:drug effects* / Q000502:physiology; D009842:Oligopeptides / Q000008:administration & dosage / Q000494:pharmacology*; D013552:Swine
chemical_list: D009842:Oligopeptides; C039537:heptanoyl-gamma-D-glutamyl-L-meso-diaminopimelyl-D-alanine