Induction of type II collagen-specific antibody production in blood lymphocyte cultures of rhesus monkeys (Macaca mulatta) with collagen-induced arthritis using the immobilized native antigen.
Peripheral blood mononuclear cells (PBMC) from Rhesus monkeys previously immunized with bovine type II collagen to induce arthritis were cultured with the same antigen. Because the native protein is poorly soluble in culture medium a heating step is often used. The antigen in this form induced PBMC proliferation, but epitopes for the induction of antibody production and arthritis were lost. To keep the native protein intact it was coated on affigel beads. With the immobilized antigen specific antibody production could be induced.
't Hart
B A
BA
Department of Chronic and Infectious Diseases, ITRI-TNO Institute, Rijswijk, The Netherlands.
Botman
C A
CA
Bakker
N P
NP
D000818:Animals; D000918:Antibody Specificity; D001168:Arthritis / Q000139:chemically induced / Q000276:immunology*; D001323:Autoantibodies / Q000096:biosynthesis*; D003094:Collagen / Q000276:immunology*; D004195:Disease Models, Animal; D006358:Hot Temperature; D007114:Immunization / Q000379:methods; D008214:Lymphocytes / Q000276:immunology*; D008253:Macaca mulatta; D012685:Sepharose / Q000031:analogs & derivatives
D001323:Autoantibodies; C055194:Affi-Gel 15; D003094:Collagen; D012685:Sepharose