Quantifying bone and liver alkaline phosphatase by the resolution of two-component inactivation data obtained with a centrifugal analyzer.
In this method we use a linear equation to resolve two-component decay data from the urea inactivation of mixtures of alkaline phosphatase (EC 3.1.3.1) of liver and bone origin. The specificity for the bone/liver isoenzyme is enhanced by including L-phenylalanine in the urea reaction to inhibit the intestinal and placental forms. Bone and liver fractions are each quantified from results of a single run on the centrifugal analyzer. Total activity and the L-phenylalanine-inhibited fraction are measured on separate runs. A simplification of this method has been used in our laboratory for 14 years in performing greater than 80,000 analyses of clinical specimens. Speed, accuracy, and precision are improved over previous methods by this mathematical solution. Data reduction is automated through the use of a personal computer interfaced to the analyzer. With such systems generally available, this method can now be suggested as suitable for routine use to separate these and perhaps other isoenzyme mixtures.
Forsman
R W
RW
Department of Laboratory Medicine & Pathology, Mayo Clinic, Rochester, MN 55905.
O'Brien
J F
JF
D000469:Alkaline Phosphatase / Q000378:metabolism*; D001842:Bone and Bones / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D010001:Osteitis Deformans / Q000201:enzymology; D010649:Phenylalanine; D012015:Reference Standards
D007527:Isoenzymes; D010649:Phenylalanine; D000469:Alkaline Phosphatase