Measurement of alkaline phosphatase of intestinal origin in plasma by p-bromotetramisole inhibition.
L-p-bromotetramisole was used to inhibit non-intestinal alkaline phosphatase (of liver or bone origin) (EC 3.1.3.1; ALP) in plasma, and intestinal ALP was measured from the uninhibited activity. The method of determination is convenient and correlated well with measurement by immunocapture assay. If carried out in parallel with wheat-germ lectin precipitation of bone ALP, subtraction of intestinal ALP activity from that of non-bone ALP in the supernatant can be used to measure the ALP that originates from the liver in men and non-pregnant women.
Kuwana
T
T
Department of Chemical Pathology and Human Metabolism, Royal Free Hospital, London.
Rosalki
S B
SB
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000097:blood*; D000911:Antibodies, Monoclonal; D001842:Bone and Bones / Q000201:enzymology; D005260:Female; D006801:Humans; D007422:Intestines / Q000201:enzymology*; D007978:Levamisole / Q000031:analogs & derivatives; D008099:Liver / Q000201:enzymology; D008297:Male; D013773:Tetramisole / Q000031:analogs & derivatives / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D007978:Levamisole; C006226:4-bromotetramisole; D013773:Tetramisole; D000469:Alkaline Phosphatase