First characterization of the mutagenic specificity of 7-methoxy-2-nitronaphtho[2,1-b]furan (R7000) in Salmonella typhimurium.
In order to investigate the mutational events induced by the very potent genotoxic agent 7-methoxy-2-nitronaphtho[2,1-b]furan (R7000), we evaluated its mutagenic potency in a battery of his- mutants of Salmonella typhimurium designed to study mutagenic specificity (Levin and Ames, 1986). Using this system we could show that R7000 is able to induce base-pair substitutions, mainly GC----TA transversions and, perhaps to a lower extent, AT----TA transversions. In addition, our results suggest that this chemical may be a very efficient inducer of base-pair deletions/insertions resulting in frameshifts. Whereas the induction of base-pair substitutions by R7000 is dependent on the presence of plasmid pKM101 carrying the mucA,B operon, the induction of base-pair deletions/insertions is independent of the presence of the plasmid.
Quillardet
P
P
Unité de Programmation Moléculaire et Toxicologie Génétique, CNRS LA271, INSERM U163, Institut Pasteur, Paris, France.
Touati
E
E
Hofnung
M
M
D000409:Alanine / Q000031:analogs & derivatives / Q000494:pharmacology; D004260:DNA Repair / Q000187:drug effects; D004361:Drug Tolerance / Q000235:genetics; D016153:Genes, Suppressor; D009152:Mutagenicity Tests; D009153:Mutagens; D009581:Nitrofurans / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics*
D009153:Mutagens; D009581:Nitrofurans; C003633:triazolealanine; C030911:2-nitro-7-methoxynaphtho(2-1b)furan; D000409:Alanine