title: Serum sensitivity and lipopolysaccharide characteristics in Bordetella bronchiseptica, B. pertussis and B. parapertussis.
abstract: The viability of four strains of Bordetella bronchiseptica, two strains of B. pertussis and one strain of B. parapertussis exposed to hyperimmune and pre-colostrum porcine serum was examined. Viable cell numbers (cfu/ml) of the B. pertussis strains and a rough strain of B. bronchiseptica (CSU-P-1) decreased by 99% and 99.99%, respectively, after exposure for 1 h to porcine hyperimmune serum. In contrast, smooth B. bronchiseptica strains and the B. parapertussis strain showed no significant decrease in viable cell numbers after the same treatment. B. bronchiseptica strain CSU-P-1 also showed a 99% decrease in viable cell numbers after exposure to pre-colostrum porcine serum for 1 h whereas the other strains tested showed no decrease in viable numbers under the same conditions. Heating the hyperimmune and pre-colostrum serum at 56 degrees C for 30 min resulted in the loss of bactericidal activity suggesting the involvement of complement in both systems. Analysis of silver-stained SDS-PAGE profiles of lipopolysaccharide (LPS) extracted from the bacterial cells indicated that the smooth strains of B. bronchiseptica and the B. parapertussis strain possessed high mol. wt O-side chain-like material, whereas the B. pertussis strains and B. bronchiseptica strain CSU-P-1 did not. Gel filtration of acid-hydrolysed LPS samples indicated two distinct carbohydrate peaks for the strains with high mol. wt O-side chain-like material, whereas the other strains each yielded one distinct peak. Western-blot analysis indicated a positive reaction for anti-B. bronchiseptica antibodies to the high mol. wt O-side chain-like material of all serum-resistant strains used in this study.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg 24061.
authors.forename: D W
authors.initials: DW
authors.lastname: Byrd
authors.forename: R M
authors.initials: RM
authors.lastname: Roop
authors.forename: H P
authors.initials: HP
authors.lastname: Veit
authors.forename: G G
authors.initials: GG
authors.lastname: Schurig
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001884:Bordetella / Q000032:analysis* / Q000254:growth & development; D001886:Bordetella pertussis / Q000032:analysis* / Q000254:growth & development; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000302:isolation & purification*; D015610:Serum Bactericidal Test; D013552:Swine
chemical_list: D008070:Lipopolysaccharides