Molecular cloning of gp 80, a glycoprotein complex secreted by kidney cells in vitro and in vivo. A link to the reproductive system and to the complement cascade.
cDNA clones coding for the gp 80 heterodimeric glycoprotein complex secreted constitutively at the apical surface of Madin-Darby canine kidney (MDCK) cells have been isolated from MDCK cDNA libraries in lambda gt11 and lambda gt10. The cloned sequences encode a polypeptide chain of 445 amino acids. The deduced amino acid sequence of the gp 80 protein reveals 80% homology to rat SGP-2, a major secretory protein of the testes epithelium and 83% homology to SP-40,40, a human complement-associated protein. SGP-2 and SP-40,40 have been proposed to be serum and seminal forms of the same protein. The sequence homology as well as the results of Southern and Northern blot analyses and immunological studies suggest that gp 80 is the canine homolog of the rat SGP-2 and the human SP-40,40. The protein is expressed in the embryonic kidney already early during organogenesis. In the adult kidney the protein has been localized along the luminal surfaces of the proximal and distal tubule and the collecting duct cells.
Hartmann
K
K
Abteilung Molekulare Genetik, J. W.-Goethe-Universität Frankfurt, Federal Republic of Germany.
Rauch
J
J
Urban
J
J
Parczyk
K
K
Diel
P
P
Pilarsky
C
C
Appel
D
D
Haase
W
W
Mann
K
K
Weller
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D005164:Factor IX / Q000235:genetics; D005170:Factor X / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D011516:Prothrombin / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D019947:Receptors, Interleukin-6; D012689:Sequence Homology, Nucleic Acid
D006023:Glycoproteins; C508603:IL6R protein, human; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D019947:Receptors, Interleukin-6; D011516:Prothrombin; D005164:Factor IX; D005170:Factor X; D004247:DNA