Axenic growth in vitro of Ancylostoma duodenale to the filariform (resting) larvae.
Large numbers of free-living stages of Ancylostoma duodenale can be grown in vitro on formalin-killed Escherichia coli supplemented with cholesterol and Dulbecco's minimum essential medium (DMEN). Eggs at 28 degrees C hatched and released first-stage larvae in 24 hr. First-stage larvae molted in 36 hr. at 28 degrees C. Second-stage larvae, molted in 2-3 days at 28 degrees C, but retained the old cuticle. Third-stage larvae characterized by the presence of a buccal cap capsule; sheath; filariform esophagus and absence of lips. Lateral alae were observed in all free-living stages.
el-Naggar
H M
HM
Department of Parasitology, Faculty of Medicine, Mansoura University, Egypt.
Abou-Zakham
A A
AA
Romia
S M
SM
Hegazi
M M
MM
el-Khouly
E S
ES
D000722:Ancylostoma / Q000254:growth & development* / Q000648:ultrastructure; D000818:Animals; D003470:Culture Media; D004926:Escherichia coli; D007814:Larva / Q000254:growth & development; D008855:Microscopy, Electron, Scanning
D003470:Culture Media