Determination of pindolol in human serum by HPLC.
A method for determining pindolol from human serum by high-performance liquid chromatography (HPLC) is presented. Pindolol is extracted into methylene chloride from 1 mL of alkalinized serum with a recovery of 87%. The organic layer is evaporated and the residue is reconstituted in mobile phase for injection into the column. Samples are eluted from a 5-micron C18 column (250 x 4.6 mm) with acetonitrile-water containing 0.1% triethylamine, pH adjusted to 3.5 with phosphoric acid (20:80 v/v). Samples as low as 2 ng/mL have been detected.
Chmielowiec
D
D
Neuropharmacology Division, Millard Fillmore Hospital, Buffalo, New York 14209.
Schuster
D
D
Gengo
F
F
D000097:Acetonitriles; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005021:Ethylamines; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008752:Methylene Chloride; D010869:Pindolol / Q000097:blood*
D000097:Acetonitriles; D005021:Ethylamines; D008752:Methylene Chloride; D010869:Pindolol; C016162:triethylamine; C032159:acetonitrile