title: Changes in AMP deaminase activities in the hearts of diabetic rats.
abstract: AMP deaminase from normal and diabetic rat hearts was separated on cellulose phosphate and quantitated by HPLC. From soluble fractions three different AMP deaminase activities, according to KCl elution from cellulose phosphate and percent of total activity were: 170 mM (85%), 250 mM (8%) and 330 mM (7%) KCl. The AMP deaminase activity which eluted with 170 mM KCl was resolved to two distinct peaks by HPLC anionic exchange. After 4 weeks of diabetes the heart enzyme profile change to: 170 mM (10%), 250 mM (75%) and 330 mM (15%). Once purified the four activities were kinetically distinct: 170 mM KCl cytosolic, AMP Km = 1.78, stimulated by ATP, GTP, NADP and strongly inhibited by NAD; 170 mM KCl mitochondria AMP Km = 17.9, stimulated by ATP, ADP; 250 mM KCl isozyme, AMP Km = 0.66, stimulated by ADP; and 330 mM KCl isozyme, AMP Km = 0.97, inhibited by ATP, NAD(P).
authors.affiliation: Department of Biology, Samford University, Birmingham, AL 35229.
authors.forename: R L
authors.initials: RL
authors.lastname: Jenkins
authors.forename: H G
authors.initials: HG
authors.lastname: McDaniel
authors.forename: L
authors.initials: L
authors.lastname: Atkins
mesh_terms: D000659:AMP Deaminase / Q000032:analysis* / Q000302:isolation & purification; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D007527:Isoenzymes / Q000032:analysis*; D007700:Kinetics; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D051381:Rats
chemical_list: D007527:Isoenzymes; D000659:AMP Deaminase