title: Endothelial cells create a hematopoietic inductive microenvironment preferential to erythropoiesis in the mouse spleen.
abstract: The spleen is an erythropoietic organ in mouse. To reconstruct a microenvironment essential for erythropoiesis in vitro, the stroma (MSS31) cell line had been established from a newborn mouse spleens. MSS31 cells exhibited properties of endothelial cells: (a) the cells showed the activity to uptake acetylated low-density lipoprotein (Ac-LDL) and (b) the cells can form a capillarylike structure by a phenotypic modulation in collagen matrices. MSS31 cells selectively supported the proliferation and differentiation of the erythroid progenitor cells by direct cell-to-cell contact in a semisolid medium in the presence of erythropoietin. These layers also supported erythrocyte maturation and enucleation of erythroblasts. This suggests that spleen endothelial cells are a new type of stromal cell with erythropoietic stimulation activity and may have a critical function in the hemopoietic inductive microenvironment of the mouse spleen.
authors.affiliation: Department of Cell Biology, Tohoku University, Sendai, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Yanai
authors.forename: T
authors.initials: T
authors.lastname: Satoh
authors.forename: M
authors.initials: M
authors.lastname: Obinata
mesh_terms: D000818:Animals; D002196:Capillaries / Q000254:growth & development; D002450:Cell Communication; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D003094:Collagen / Q000378:metabolism; D004727:Endothelium / Q000166:cytology* / Q000378:metabolism; D015672:Erythroid Precursor Cells / Q000502:physiology*; D004920:Erythropoiesis; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D013154:Spleen / Q000166:cytology*
chemical_list: D008077:Lipoproteins, LDL; D003094:Collagen