Na(+)-dependent succinate uptake in Corynebacterium glutamicum.
Succinate is effectively taken up by washed cells of Corynebacterium glutamicum. The apparent Km value of uptake is about 150 microM and the Vmax 4-7 nmol (mg dry weight)-1 min-1 and uptake can be competetively inhibited by fumarate and oxaloacetate. The activation energy was determined to be 50 kJ/mol. The transport activity is clearly dependent on the presence of Na+ ions in the incubation medium and on the membrane potential and has a pH optimum around 8.5. It is concluded that succinate is taken up in C. glutamicum via a specific carrier by a secondary active, Na+ coupled mechanism.
Ebbighausen
H
H
Institut für Biotechnologie 1, Forschungszentrum Jülich GmbH, Jülich, F.R.G.
Weil
B
B
Krämer
R
R
D001667:Binding, Competitive; D003352:Corynebacterium / Q000187:drug effects / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D005650:Fumarates / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008564:Membrane Potentials; D012964:Sodium / Q000494:pharmacology*; D013386:Succinates / Q000378:metabolism*; D019802:Succinic Acid
D005650:Fumarates; D013386:Succinates; C032005:fumaric acid; D012964:Sodium; D019802:Succinic Acid