The serological diagnosis of human hydatid disease by time-resolved fluoroimmunoassay.
The use of a new immunoassay, time-resolved fluoroimmunoassay (TR-FIA), in the diagnosis of human hydatid disease has been evaluated. This technique, which is based on the labelling of antibodies with europium (Eu), was compared with a well-established method, the enzyme linked immunosorbent assay (ELISA). Of 102 patients with hydatid disease, 97 (95.1%) were positive according to TR-FIA and 83 (81.4%) according to ELISA. The rate of non-specificity for other parasitic infections (n = 206) was 8.7% for TR-FIA and 17.5% for ELISA. It is concluded that TR-FIA is more sensitive and more specific than ELISA in the diagnosis of human hydatid disease.
Aceti
A
A
Institute of the Clinic of Tropical and Infectious Diseases, La Sapienza University, Rome, Italy.
Pennica
A
A
Teggi
A
A
Grilli
A
A
Caferro
M
M
Celestino
D
D
Leri
O
O
Sebastiani
A
A
De Rosa
F
F
D000818:Animals; D004443:Echinococcosis / Q000175:diagnosis*; D004444:Echinococcosis, Hepatic / Q000175:diagnosis; D004445:Echinococcosis, Pulmonary / Q000175:diagnosis; D004446:Echinococcus / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005063:Europium; D005069:Evaluation Studies as Topic; D015200:Fluoroimmunoassay / Q000379:methods*; D006801:Humans
D005063:Europium