Lowered superoxide dismutase in highly metastatic B16 melanoma cells.
Superoxide dismutases (SOD), which are enzymes scavenging the superoxide radical, were studied in two variant lines of the B16 melanoma: B16F1 with low metastatic potential and B16F10 with high metastatic potential. SOD activity was measured by a method utilizing reduction in the chemiluminescence of luminol. Using cell free extracts it was shown that the highly metastatic B16F10 cell line has a SOD activity lower (20.70 +/- 3.07) units/mg protein, n = 8, than that of the less metastatic B16F1 cell line (81.38 +/- 6.78) units/mg protein, n = 8. Acrylamide gel electrophoresis suggested that Mn-SOD activity is higher in B16F1 cells.
Kwee
J K
JK
Centro de Pesquisa Básica, Instituto Nacional de Câncer Praça Cruz Vermelha, Rio de Janeiro, Brasil.
Mitidieri
E
E
Affonso
O R
OR
D000818:Animals; D066298:In Vitro Techniques; D008546:Melanoma, Experimental / Q000201:enzymology*; D051379:Mice; D009362:Neoplasm Metastasis; D013482:Superoxide Dismutase / Q000378:metabolism*
D013482:Superoxide Dismutase