title: Multipoint linkage analysis of DXS369 and DXS304 in fragile X families.
abstract: Diagnosis of carriers of the fragile-X mental retardation gene is hampered by the paucity of tightly linked DNA markers. Recently, 2 new DNA markers RN1 (DXS369) and U6.2 (DXS304) have become available. Both markers are tightly linked to the fragile-X locus, but their location relative to the fragile site was not known with certainty. We have tested these new markers in a multipoint linkage analysis of 26 fragile-X families typed for DXS105 as a proximal marker and DXS52 as a distal marker. Our results establish the order DXS105-DXS369-fra(X)-DXS304-DXS52, which is in agreement with physical mapping results.
authors.affiliation: Department of Human Genetics, University Hospital Nijmegen, The Netherlands.
authors.forename: B A
authors.initials: BA
authors.lastname: van Oost
authors.forename: A
authors.initials: A
authors.lastname: Smits
authors.forename: J C
authors.initials: JC
authors.lastname: Dreesen
authors.forename: D
authors.initials: D
authors.lastname: Smeets
authors.forename: L
authors.initials: L
authors.lastname: Perdon
authors.forename: C A
authors.initials: CA
authors.lastname: van Bennekom
authors.forename: N
authors.initials: N
authors.lastname: Dahl
authors.forename: E
authors.initials: E
authors.lastname: Bakker
authors.forename: B A
authors.initials: BA
authors.lastname: Oostra
mesh_terms: D004247:DNA / Q000032:analysis; D015342:DNA Probes; D005260:Female; D005600:Fragile X Syndrome / Q000175:diagnosis / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D008126:Lod Score; D008297:Male; D011995:Recombination, Genetic
chemical_list: D015342:DNA Probes; D005819:Genetic Markers; D004247:DNA