title: Multiple mRNA coding for phospholipid-transfer protein from Zea mays arise from alternative splicing.
abstract: We have isolated a novel cDNA coding for maize phospholipid-transfer protein. The cDNA sequence is similar to the first one obtained by Tchang et al. [J. Biol. Chem. 263 (1988) 16849-16855] differing only by a mslal number of nucleotide substitutions and insertions. One of these insertions is 74 bp long and is flanked by consensus intron splicing sequences. The protein coded by the two cDNA has identical amino acids except in the C terminus. This difference derived from the presence of the 74-bp insert. The possible existence of an alternative splicing mechanism that could introduce heterogeneity in the sequence of these proteins is proposed.
authors.affiliation: Laboratoire de Physiologie Cellulaire et Moléculaire, URA1180-CNRS, Université P. et M. Curie, Paris, France.
authors.forename: V
authors.initials: V
authors.lastname: Arondel
authors.forename: F
authors.initials: F
authors.lastname: Tchang
authors.forename: B
authors.initials: B
authors.lastname: Baillet
authors.forename: F
authors.initials: F
authors.lastname: Vignols
authors.forename: F
authors.initials: F
authors.lastname: Grellet
authors.forename: M
authors.initials: M
authors.lastname: Delseny
authors.forename: J C
authors.initials: JC
authors.lastname: Kader
authors.forename: P
authors.initials: P
authors.lastname: Puigdomènech
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015723:Gene Library; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D049294:Phospholipid Transfer Proteins; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D003313:Zea mays / Q000235:genetics*
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D049294:Phospholipid Transfer Proteins; D012333:RNA, Messenger; D004247:DNA