Three novel molecular forms of biliary glycoprotein deduced from cDNA clones from a human leukocyte library.
Three cDNA clones that encode three novel variants of biliary glycoprotein a (BGPa), a glycoprotein belonging to the CEA gene family, were identified in a human leukocyte cDNA library. The domain structures of the predicted proteins of the three clones W211, W233 and W239 are N-A1-B1-A2, N-A1-B1 and N-A1-B1-C, respectively; they lack the transmembrane and cytoplasmic domains that exist in the four BGP species (BGPa, BGPb, BGPc and BGPd) previously reported. Their sequences from N to B1 or to A2 are virtually identical to those of BGPa-d. Comparison with the genomic sequence for BGPa-d suggested that these three new BGP variants as well as BGPa-d are generated from the same single gene by alternative splicing of RNA.
Kuroki
M
M
Department of Biochemistry, School of Medicine, Fukuoka University, Japan.
Arakawa
F
F
Matsuo
Y
Y
Oikawa
S
S
Nakazato
H
H
Matsuoka
Y
Y
D015703:Antigens, CD; D001483:Base Sequence; D002272:Carcinoembryonic Antigen / Q000235:genetics / Q000276:immunology; D015815:Cell Adhesion Molecules; D003001:Cloning, Molecular; D003062:Codon; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes; D014644:Genetic Variation; D015698:Genomic Library; D006023:Glycoproteins / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008969:Molecular Sequence Data; D012326:RNA Splicing
D015703:Antigens, CD; C069847:CD66 antigens; D002272:Carcinoembryonic Antigen; D015815:Cell Adhesion Molecules; D003062:Codon; D015342:DNA Probes; D006023:Glycoproteins; D004247:DNA