title: Identification of toxigenic Clostridium botulinum type E by enzyme immunoassay.
abstract: A sensitive double-sandwich enzyme immunoassay for identification of toxigenic Clostridium botulinum type E was developed with a type-E-specific antitoxin. The antitoxin was produced in a rabbit, following inoculation of minute quantities of type-E toxoid. The toxoid was prepared from a commercially available toxin by using a detoxification method. Cross-reactivity of the antitoxin with C. botulinum types A and B was avoided by absorption of the serum with alum-precipitated toxoids A and B. The immunoassay proved to be specific for C. botulinum type E because it did not react with culture filtrates of various Clostridium species, including nontoxigenenic "E-like" organisms and Clostridium sporogenes.
authors.affiliation: Department of Medical Laboratory Sciences, Florida International University, Miami 33199.
authors.forename: M
authors.initials: M
authors.lastname: Dezfulian
authors.forename: J G
authors.initials: JG
authors.lastname: Bartlett
mesh_terms: D000818:Animals; D001905:Botulinum Toxins / Q000096:biosynthesis* / Q000276:immunology; D003014:Clostridium botulinum / Q000302:isolation & purification* / Q000378:metabolism; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera / Q000276:immunology; D007114:Immunization; D007117:Immunization, Secondary; D008297:Male; D011817:Rabbits; D014121:Toxoids / Q000276:immunology
chemical_list: D007106:Immune Sera; D014121:Toxoids; D001905:Botulinum Toxins