cDNA encoding the major mite allergen Der f II.
cDNA encoding the major house dust mite allergen Der f II from Dermatophagoides farinae was amplified using the polymerase chain reaction and cloned into E. coli. It encoded a 129-residue protein with a calculated molecular weight of 14,021 D and had the expected high homology (88%) with Der p II including the absence of N-glycosylation sites and conserved cysteine residues. These results are consistent with the high degree of antibody crossreactivity and may help identify the differences in T-cell epitopes revealed for these molecules so far.
Trudinger
M
M
Western Australia Research Institute for Child Health, Princess Margaret Hospital, Perth.
Chua
K Y
KY
Thomas
W R
WR
D000485:Allergens / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D004391:Dust; D008925:Mites / Q000276:immunology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D000485:Allergens; D004391:Dust; D004247:DNA