Effects of a fecapentaene on protein kinase C.
Protein kinase C (PKC) is a Ca2(+)- and phospholipid-dependent serine and threonine protein kinase which binds and is activated by tumor promoters such as the phorbol ester 12-0-tetradecanoylphorbol-13-acetate (TPA). PKC can be activated in vitro by phosphatidylserine (PS) plus Ca2+. We report here that the compound fecapentaene-12 can replace the requirement for PS in the activation of PKC by Ca2+. In addition, at low concentrations fecapentaene-12 can enhance the activation of PKC by Ca2+ and PS. It can also either enhance or inhibit activation of PKC by the tumor promoter teleocidin, depending on the assay conditions. These results are of interest since fecapentaene is known to be a potent mutagen that is produced by Bacteroides species present in the lumen of the human colon. The present studies raise the possibility that this compound might also play a role in colon cancer by altering the activity of PKC.
Hoshina
S
S
Comprehensive Cancer Center, Columbia University, New York, NY 10032.
Ueffing
M
M
Morotomi
M
M
Weinstein
I B
IB
D000818:Animals; D001921:Brain / Q000201:enzymology; D002118:Calcium / Q000494:pharmacology; D004789:Enzyme Activation; D007700:Kinetics; D009153:Mutagens / Q000494:pharmacology*; D010718:Phosphatidylserines / Q000494:pharmacology; D011090:Polyenes / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D009153:Mutagens; D010718:Phosphatidylserines; D011090:Polyenes; C037357:1-(1-glycero)dodeca-1,3,5,7,9-pentaene; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium