Analysis of some eicosanoids by continuous-flow fast atom bombardment mass spectrometry.
Continuous-flow fast atom bombardment has been used to analyze eicosanoids by selected-ion monitoring on a sector-field mass spectrometer operating in the negative-ion mode. The method has been optimized with respect to solvent composition and flow-rates. Detection limits were below 50 pg, and under optimal conditions a linear relationship between response and amount of substance was achieved. The method was successfully applied to the analysis of two spiked urine samples.
Justesen
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U
Department of Chemistry, Odense University, Denmark.
Bojesen
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G
D015237:Dinoprost / Q000652:urine; D015232:Dinoprostone / Q000652:urine; D015777:Eicosanoids / Q000652:urine*; D005990:Glycerol / Q000031:analogs & derivatives / Q000032:analysis; D006801:Humans; D011453:Prostaglandins / Q000652:urine; D012997:Solvents; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013929:Thromboxane B2 / Q000652:urine
D015777:Eicosanoids; D011453:Prostaglandins; D012997:Solvents; D013929:Thromboxane B2; C009465:thioglycerol; D015237:Dinoprost; D015232:Dinoprostone; D005990:Glycerol