Polymorphisms generated by arbitrarily primed PCR in the mouse: application to strain identification and genetic mapping.
Polymorphisms in genomic fingerprints generated by arbitrarily primed PCR (AP-PCR) can distinguish between strains of almost any organism. We applied the technique to the mouse (Mus musculus). The characteristic differences in the AP-PCR genomic fingerprints between strains will be of value in strain identification and verification. Using one primer, we genetically mapped four polymorphisms in a set of C57BL/6J x DBA/2J recombinant inbreds. One of these polymorphisms is a length variant. The method will allow rapid genetic mapping of DNA polymorphisms without Southern blotting.
Welsh
J
J
California Institute of Biological Research, La Jolla 92037.
Petersen
C
C
McClelland
M
M
D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D016172:DNA Fingerprinting; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011995:Recombination, Genetic; D013045:Species Specificity
D004247:DNA