title: Characterization of a monoclonal antibody directed against the phosphotyrosine kinase p56lck, in human T cells.
abstract: A monoclonal antibody (anti-p56lck) was generated against a fusion protein containing the residues 145-509 of the human p56lck, a lymphocyte-specific membrane-associated protein tyrosine kinase. The involvement of this enzyme in T-cell transmembrane signalling seems to be an early and crucial event during T-cell receptor-mediated activation. We have produced a monoclonal antibody which recognizes p56lck in free form and when associated with CD4. It functions in western blot analysis and is capable of selectively blocking auto-phosphorylation of this kinase. This monoclonal antibody should be useful for investigating the role of p56lck in T-cell activation.
authors.affiliation: Department of Immunology, Karolinska Institute, Stockholm, Sweden.
authors.forename: I J
authors.initials: IJ
authors.lastname: Ansotegui
authors.forename: S C
authors.initials: SC
authors.lastname: Chow
authors.forename: M
authors.initials: M
authors.lastname: Jeddi-Tehrani
authors.forename: S
authors.initials: S
authors.lastname: Meloche
authors.forename: A
authors.initials: A
authors.lastname: Pawson
authors.forename: R P
authors.initials: RP
authors.lastname: Sekaly
authors.forename: T W
authors.initials: TW
authors.lastname: Mak
authors.forename: H
authors.initials: H
authors.lastname: Wigzell
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D015704:CD4 Antigens / Q000276:immunology; D006801:Humans; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D010766:Phosphorylation / Q000187:drug effects; D011233:Precipitin Tests; D011505:Protein-Tyrosine Kinases / Q000276:immunology*; D015398:Signal Transduction / Q000187:drug effects; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D011505:Protein-Tyrosine Kinases; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck)