High concentration of a gastrin releasing peptide-like immunoreactive substance in pregnant human milk.
The level of a gastrin releasing peptide-like immunoreactive substance (GRP-IS) in human milk and plasma during late pregnancy and after delivery was measured. GRP-IS in milk increased during late pregnancy (1.3 nM at 39 weeks) and decreased after delivery (100 pM). GRP-IS in plasma during late pregnancy and after delivery was much lower (below 2 pM). By using HPLC and gel-filtration chromatography, two peaks of GRP-IS were purified. The one was coeluted with GRP (18-27) and the other was a prohormone. The GRP-IS in milk during pregnancy was mostly composed of proGRP. These results suggest that GRP may be produced and secreted in mammary glands.
Takeyama
M
M
Department of Hospital Pharmacy, Medical College of Oita, Japan.
Kondo
K
K
Takayama
F
F
Kondo
R
R
Murata
H
H
Miyakawa
I
I
D001940:Breast / Q000378:metabolism*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005260:Female; D019886:Gastrin-Releasing Peptide; D006801:Humans; D008895:Milk, Human / Q000378:metabolism; D010455:Peptides / Q000097:blood / Q000378:metabolism*; D011247:Pregnancy
D010455:Peptides; D019886:Gastrin-Releasing Peptide