Biochemical characterization of reperfusion pancreatitis in porcine pancreatic allografts after six hours of cold storage.
Biochemical signs of pancreatitis in plasma and pancreatic exudates were determined in 22 pigs subjected to pancreatic allograft transplantation after the graft had been in cold storage for 6 hr. Two perfusion and preservation media were used. We found signs of protease activation in the pancreatic exudate during the first hour after reperfusion. The local protease protection barrier was, however, not broken and no plasma changes indicating pancreatitis were seen during this period. On the first and second postoperative days, mild biochemical signs of pancreatitis were seen in the plasma, including a decrease in kininogen and C3 concentration as well as in plasma kallikrein inhibitory activity and the appearance of trypsin-protease inhibitor complexes. No correlation was seen between these biochemical signs of pancreatitis and graft appearance or function, indicating that the reperfusion pancreatitis seen after 6 hr of cold storage is of minor significance. No significant differences were seen between the two preservation media used (Perfadex and EuroCollins solution).
Källén
R
R
Department of Surgery, Malmö General Hospital, University of Lund, Sweden.
Borgström
A
A
D000818:Animals; D003176:Complement C3 / Q000032:analysis; D015925:Cryopreservation; D003470:Culture Media; D007704:Kininogens / Q000276:immunology; D016035:Pancreas Transplantation / Q000502:physiology*; D010195:Pancreatitis / Q000209:etiology / Q000378:metabolism*; D011480:Protease Inhibitors / Q000097:blood; D015424:Reperfusion / Q000009:adverse effects; D012709:Serum Albumin / Q000276:immunology; D013552:Swine; D013997:Time Factors; D014184:Transplantation, Homologous / Q000502:physiology; D014357:Trypsin / Q000276:immunology
D003176:Complement C3; D003470:Culture Media; D007704:Kininogens; D011480:Protease Inhibitors; D012709:Serum Albumin; D014357:Trypsin