title: Biochemical analysis of bovine T cell antigens with workshop monoclonal antibodies.
abstract: The relative molecular weight (Mr) of the CD1, CD2, CD4, CD5, CD6, CD8 and additional molecules identified by monoclonal antibodies (mAb) submitted to the workshop was determined using 125I surface-labelled leukocytes. Of 37 mAb precipitating lymphocyte surface molecules, 33 had Mr correlating with their clusters of differentiation. Further, we were able to determine Mr for three "undefined T lymphocyte" antibodies. Of the 20 mAb that did not precipitate a molecule from the surface of leukocytes, one was non-reactive as determined by flow microfluorimetry. The inability to precipitate a molecule from the remaining cells was probably due to lower affinities of those antibodies and conformational changes in the antigen(s) following lysis of the leukocytes.
authors.affiliation: Department of Veterinary Science, University of Wisconsin-Madison 53706.
authors.forename: K L
authors.initials: KL
authors.lastname: O'Reilly
authors.forename: L
authors.initials: L
authors.lastname: Eskra
authors.forename: G A
authors.initials: GA
authors.lastname: Splitter
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000737:chemistry*; D002417:Cattle / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D011233:Precipitin Tests
chemical_list: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte