Calcium oscillation associated with reduced protein kinase C activities in ras-transformed NIH3T3 cells.
We show here novel intracellular Ca2+ oscillation in v-K-ras-transformed NIH3T3 cells induced by mitogenic peptide hormones, bradykinin and bombesin, as well as fetal calf serum. Induction of the Ca2+ oscillation is strongly correlated with the malignant properties and inversely with PKC activities in vitro and in vivo. These results suggest that the mitogen-induced Ca2+ oscillation is negatively regulated by PKC, which modulates Ca2+ influx in v-K-ras-transformed NIH3T3 cells.
Fu
T
T
Department of Biochemistry, Gifu University School of Medicine, Japan.
Sugimoto
Y
Y
Oki
T
T
Murakami
S
S
Okano
Y
Y
Nozawa
Y
Y
D000818:Animals; D001839:Bombesin / Q000494:pharmacology*; D001920:Bradykinin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D015180:Electrophoresis, Gel, Two-Dimensional; D011905:Genes, ras; D007700:Kinetics; D051379:Mice; D010750:Phosphoproteins / Q000302:isolation & purification; D011493:Protein Kinase C / Q000378:metabolism*
D010750:Phosphoproteins; D011493:Protein Kinase C; D001839:Bombesin; D001920:Bradykinin; D002118:Calcium