PCR primers for human chromosomes: reagents for the rapid analysis of somatic cell hybrids.
Rapid analysis of somatic cell hybrids can be facilitated by using the polymerase chain reaction (PCR) to assay for genes assigned to specific human chromosomes. We describe PCR primer pairs for genes on the short and long arms of the 22 autosomes and the X chromosome. Some of the primers were designed from the 3' untranslated region of cDNA sequences, whereas others were derived from genomic sequence. Each primer set was tested for its specificity and mapped to a chromosome by screening a somatic cell hybrid panel. Two of the primer pairs (APOC2 and G6PD) detect CA dinucleotide repeat polymorphisms.
Theune
S
S
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284-7762.
Fung
J
J
Todd
S
S
Sakaguchi
A Y
AY
Naylor
S L
SL
D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping / Q000379:methods*; D002877:Chromosomes, Human; D004587:Electrophoresis, Agar Gel; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid
D009838:Oligodeoxyribonucleotides