title: The effect of salt on the Michaelis Menten constant of the HIV-1 protease correlates with the Hofmeister series.
abstract: The effect of different types of salt on the proteolytic activity of HIV-1 protease was studied. At a similar ionic strength, the enzyme activity changed according to the salting out effect of the ions used (Hofmeister series). Kinetic studies showed that a stronger salting out effect of the ions rather than the higher ionic strength per se increased the affinity to the substrate (Km) but in general did not alter the Kcat value.
authors.affiliation: Laboratory of Molecular Virology and Carcinogenesis, NCI-Frederick Cancer Research and Development Center, MD 21702.
authors.forename: E M
authors.initials: EM
authors.lastname: Wondrak
authors.forename: J M
authors.initials: JM
authors.lastname: Louis
authors.forename: S
authors.initials: S
authors.lastname: Oroszlan
mesh_terms: D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000235:genetics; D016333:HIV Protease / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D012492:Salts / Q000494:pharmacology*; D013379:Substrate Specificity / Q000187:drug effects
chemical_list: D012492:Salts; D016333:HIV Protease