Immunological characterization of deglycosylated human and swine trachea and Cowper's gland mucin glycoproteins.
Antibodies were raised in rabbits against purified swine and human trachea and Cowper's gland mucin glycoproteins and their deglycosylated polypeptide chains. Three monospecific antibody fractions that recognize the carbohydrate, the deglycosylated or unglycosylated regions of the polypeptide chains in these glycoproteins, were isolated by immunoaffinity chromatography. The human and swine trachea mucin glycoproteins showed extensive immunological homology in both their carbohydrate and polypeptide chains. The carbohydrate chains and unglycosylated region of the polypeptide chain in Cowper's gland mucin glycoprotein showed little or no cross-reaction with comparable regions in respiratory mucin glycoproteins. However, the polypeptide chains in the deglycosylated regions of all three mucin glycoproteins showed extensive homology. Five bands with molecular masses ranging from 40 to 46 kDa that differed by a constant molecular mass of approximately 1.5 kDa were detected in hydrolysates of all of the polypeptide chains after treatment with S. aureus V8 protease. Monospecific antibodies to the deglycosylated region of these chains reacted with the peptides, whereas those directed against the unglycosylated region did not. The results suggest that these chains contain tandem repeating sequences of amino acids.
Sangadala
S
S
Department of Biochemistry, University of Georgia, Athens 30602.
Brewer
J M
JM
Mendicino
J
J
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D002030:Bulbourethral Glands / Q000378:metabolism*; D002846:Chromatography, Affinity; D003429:Cross Reactions; D003550:Cystic Fibrosis / Q000503:physiopathology; D004797:Enzyme-Linked Immunosorbent Assay; D006031:Glycosylation; D006801:Humans; D008297:Male; D009077:Mucins / Q000032:analysis* / Q000276:immunology; D009130:Muscle, Smooth / Q000378:metabolism*; D013183:Sputum / Q000737:chemistry; D013552:Swine; D014132:Trachea / Q000378:metabolism*
D000596:Amino Acids; D000906:Antibodies; D000911:Antibodies, Monoclonal; D009077:Mucins