Mechanistic stoichiometry of mitochondrial oxidative phosphorylation.
P/O ratios of rat liver mitochondria were measured with particular attention to systematic errors. Corrections for energy loss during oxidative phosphorylation were made by measurement of respiration as a function of mitochondrial membrane potential. The corrected values were close to 1, 0.5, and 1 at the three coupling sites, respectively. These values are consistent with recent measurements of mitochondrial proton transport.
Hinkle
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Section of Biochemistry, Molecular, and Cell Biology, Cornell University, Ithaca, New York 14853.
Kumar
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MA
Resetar
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Harris
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D000244:Adenosine Diphosphate / Q000378:metabolism; D000818:Animals; D004579:Electron Transport; D008314:Malonates / Q000494:pharmacology; D008564:Membrane Potentials; D008930:Mitochondria, Liver / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats
D008314:Malonates; D010710:Phosphates; D009243:NAD; D000244:Adenosine Diphosphate; C030290:malonic acid; D010100:Oxygen