[The characteristics of the regulation of the cellular proliferation of embryonic rat fibroblasts transformed by E1A+c-Ha-ras oncogenes].
The ability of growth factors (GF) to stimulate proliferation of rat embryo fibroblast (REF) lines immortalized by E 1A oncogene and transformed by complementing E 1A and c-Ha-ras oncogenes (mutant form) was studied. Unlike untreated REF, those immortalized by E 1A oncogene required less serum and GF to proliferate. Proliferation could be stimulated by specific GF alone. Serum appeared to be capable of stimulating proliferation of cells transformed by E 1A + c-Ha-ras oncogenes; however, both GF and proteinases C and A activators failed to exert such effect. Transferrin, usually required by normal cells in late G1-phase, shared the stimulating effect on E 1A+c-Ha-ras-transformed cells. To summarize, E 1A+c-Ha-ras oncogene-transformed REF can grow independently of GF but still require transferrin.
Blagosklonnyĭ
M V
MV
Darieva
Z A
ZA
Pospelova
T V
TV
D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000235:genetics*; D002999:Clone Cells / Q000166:cytology / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D011905:Genes, ras / Q000187:drug effects / Q000502:physiology*; D006133:Growth Substances / Q000494:pharmacology; D051381:Rats; D013268:Stimulation, Chemical
D006133:Growth Substances