title: Microbore liquid chromatography coupled to a flow fast atom bombardment probe for the on-line detection of the Tyr-Pro cleavage of a nonapeptide by recombinant HIV-1 protease.
abstract: The nonapeptide Val-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln has been reported as a model substrate for an aspartyl protease produced by the human immunodeficiency virus (HIV-1). Cleavage of this peptide at the Tyr-Pro linkage to produce tetra- and pentapeptide fragments is the basis of high-performance liquid chromatographic assays to detect HIV-1 protease activity. Confirmation of the cleavage site has been proved by using microbore liquid chromatography coupled to a dynamic fast atom bombardment interface. Comparison with fortified control incubates indicates that an approximate stoichiometric amount of the tetrapeptide was formed from the nonapeptide, confirming that the cleavage of the substrate by HIV-1 protease is both specific and quantitative.
authors.affiliation: Pfizer Central Research, Sandwich, Kent, U.K.
authors.forename: S M
authors.initials: SM
authors.lastname: Cole
authors.forename: P V
authors.initials: PV
authors.lastname: Macrae
authors.forename: J R
authors.initials: JR
authors.lastname: Merson
authors.forename: F S
authors.initials: FS
authors.lastname: Pullen
authors.forename: D J
authors.initials: DJ
authors.lastname: Rance
mesh_terms: D000595:Amino Acid Sequence; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D016333:HIV Protease / Q000235:genetics; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis*; D011392:Proline / Q000032:analysis*; D011994:Recombinant Proteins / Q000235:genetics; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013056:Spectrophotometry, Ultraviolet; D014443:Tyrosine / Q000032:analysis*
chemical_list: D010455:Peptides; D011994:Recombinant Proteins; D014443:Tyrosine; D011392:Proline; D016333:HIV Protease