Quantitation of bovine immunoglobulins in culture fluids by use of sandwich radioimmunoassay with monoclonal antibodies.
Bovine immunoglobulin isotype-specific murine monoclonal antibodies were used in sandwich radioimmunoassays to detect and quantitate bovine IgG1, IgG2, IgM, and IgA in culture fluids. The concentrations of bovine immunoglobulins in unknown samples were extrapolated from standard curves generated with bovine monoclonal immunoglobulins. The lowest detection limits for the bovine immunoglobulin isotypes ranged from 65 to 270 ng/ml.
Srikumaran
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S
Department of Veterinary Science, University of Nebraska, Lincoln 68583-0905.
Kluever
E A
EA
Onisk
D V
DV
Hariharan
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D003470:Culture Media / Q000737:chemistry; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007136:Immunoglobulins / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods / Q000662:veterinary*
D000911:Antibodies, Monoclonal; D003470:Culture Media; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins