An internal domain of the hepatitis B virus X antigen is necessary for transactivating activity.
A number of deletion mutants of the hepatitis B viral X antigen had been constructed and assayed for their ability to transactivate heterologous viral regulatory elements. Neither the N-terminal nor the C-terminal amino acid residues were required for transactivating activity. Transactivating activity that resided within amino acid residues 32 to 148 of the X antigen did not show any target DNA sequence or cell line specificity.
Ritter
S E
SE
Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46223.
Whitten
T M
TM
Quets
A T
AT
Schloemer
R H
RH
D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D015967:Gene Expression Regulation, Viral; D006515:Hepatitis B virus / Q000235:genetics*; D015183:Restriction Mapping; D013329:Structure-Activity Relationship; D015534:Trans-Activators / Q000235:genetics*; D054334:Viral Regulatory and Accessory Proteins
D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein