Identification of a co-stimulatory factor for human T cell proliferation.
A factor enhancing proliferation of human peripheral blood lymphocytes (PBL) was identified in the culture supernatant of a human myeloid cell line HL-60 after ion exchange separation. Partially purified lymphocyte growth enhancing factor (LGEF) was found to enhance mitogen-stimulated PBL or purified T cell proliferation in a dose dependent fashion. LGEF alone did not stimulate PBL or T cell proliferation and its activity was dependent on the presence of mitogen. LGEF mediated T cell proliferation was neither accompanied by an increase in the level of IL-2 receptor expression, nor was dependent on the presence of monocytes. In ELISA assay antibodies against IL-1, IL-2, IL-3 and IL-6 did not recognize the LGEF preparation. The comparison of LGEF with other lymphokines is discussed.
Abolhassani
M
M
Department of Medicine, New York Medical College, Valhalla 10595.
Chiao
J W
JW
D002460:Cell Line; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007951:Leukemia, Myeloid / Q000209:etiology*; D008213:Lymphocyte Activation / Q000502:physiology*; D008222:Lymphokines / Q000032:analysis* / Q000502:physiology; D015375:Receptors, Interleukin-2 / Q000032:analysis
D008222:Lymphokines; D015375:Receptors, Interleukin-2; C043695:lymphocyte proliferation potentiating factors