title: Comparison of the binding characteristics to BHK-21 cells of viruses representing the two Theiler's virus neurovirulence groups.
abstract: The binding characteristics of the highly virulent GDVII and less virulent BeAn strains of Theiler's murine encephalomyelitis viruses (TMEV) to whole BHK-21 cells were determined using a direct viral binding assay. The overall rates of association and dissociation of BeAn and GDVII viruses were similar. Using a saturation binding assay intended for multivalent ligands, such as picornaviruses, the number of binding sites per cell was calculated as 1.6 x 10(5). Competitive binding assays with both viruses showed one-way blocking. In addition, treatment of cell monolayers with neuraminidase reduced binding of BeAn virus by 90% but did not affect GDVII binding. Wheat germ agglutinin, a lectin which blocks binding to sialic acid and N-acetylglucosamine residues, substantially reduced binding of radiolabeled GDVII and BeAn viruses. Treatment of asialylated cells with O-glycanase further reduced the binding of BeAn virus, suggesting that O-linked oligosaccharides are involved in viral binding. These results suggest members of the two TMEV virulence groups share a common receptor but bind it differently.
authors.affiliation: Department of Neurology, University of Colorado Health Sciences Center, Denver 80262.
authors.forename: C
authors.initials: C
authors.lastname: Fotiadis
authors.forename: D R
authors.initials: DR
authors.lastname: Kilpatrick
authors.forename: H L
authors.initials: HL
authors.lastname: Lipton
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D006224:Cricetinae; D010450:Endopeptidases / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000378:metabolism; D037102:Lectins / Q000494:pharmacology; D004680:Maus Elberfeld virus / Q000378:metabolism / Q000472:pathogenicity*; D051379:Mice; D009474:Neurons / Q000382:microbiology; D010740:Phospholipases / Q000494:pharmacology; D011991:Receptors, Virus / Q000737:chemistry / Q000378:metabolism*
chemical_list: D037102:Lectins; D011991:Receptors, Virus; D010740:Phospholipases; D006026:Glycoside Hydrolases; C020668:glycanase; D010450:Endopeptidases