title: Kinase activity and protein phosphorylation in control and malignant hyperthermic skeletal muscle.
abstract: 1. Native 6% Laemmli gels were used to resolve 7 protein kinase activity bands in control and malignant hyperthermia (MH)-susceptible porcine and human skeletal muscle extracts. 2. MH-susceptible samples were consistently more active than the controls. 3. Following halothane treatment, a 43 kDa component displayed increased phosphorylation by a calcium-calmodulin dependent kinase in MH-susceptible vs control human samples. 4. Increased phosphorylation of additional endogenous protein components of molecular mass 116 and 60 kDa was observed.
authors.affiliation: Department of Medical Biochemistry, University of the Witwatersrand Medical School, Parktown, South Africa.
authors.forename: M
authors.initials: M
authors.lastname: Joffe
authors.forename: N
authors.initials: N
authors.lastname: Savage
authors.forename: C
authors.initials: C
authors.lastname: du Sautoy
authors.forename: G
authors.initials: G
authors.lastname: Mitchell
authors.forename: H
authors.initials: H
authors.lastname: Isaacs
mesh_terms: D000818:Animals; D003600:Cytosol / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D066298:In Vitro Techniques; D008305:Malignant Hyperthermia / Q000378:metabolism* / Q000503:physiopathology; D008565:Membrane Proteins / Q000378:metabolism; D008970:Molecular Weight; D009119:Muscle Contraction; D009124:Muscle Proteins / Q000302:isolation & purification / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism* / Q000502:physiology; D010766:Phosphorylation; D011494:Protein Kinases / Q000302:isolation & purification / Q000378:metabolism*; D013552:Swine
chemical_list: D008565:Membrane Proteins; D009124:Muscle Proteins; D011494:Protein Kinases