Role of a GC-rich motif in transcription regulation of the adenovirus type 2 IVa2 promoter which lacks typical TATA-box element.
Promoter of the adenovirus type 2 IVa2 (IVa2) gene does not have a TATA-box element, hence it is considered to be a model of cellular TATA-less promoters. In vitro transcription of the IVa2 promoter in HeLa cell extracts identified a proximal cis-element around -40 relative to the transcription start site. DNase I footprint analysis revealed the presence of a DNA-binding factor which interacts with sequences ACCCCTCCCACTTAG at -50. Competition footprint analysis and sequence comparisons showed that the IVa2 proximal promoter element is equivalent to the GC-box, the Sp1-binding site.
Tamura
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T
Division of Behavior and Neurobiology, National Institute for Basic Biology, Okazaki, Japan.
Mikoshiba
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K
D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D016385:TATA Box; D014158:Transcription, Genetic
D004279:DNA, Viral; D004268:DNA-Binding Proteins; D003850:Deoxyribonuclease I