title: Stage and lineage-regulated expression of two hsp90 transcripts during mouse germ cell differentiation and embryogenesis.
abstract: The expression of members of the heat shock protein 90 (hsp90) gene family during testicular and embryonic development was investigated. Two different hsp90 transcripts were detected in RNA from mouse testis, approximately 3.2 kb and 2.9 kb in size, and were shown to exhibit cellular and developmental stage specificity of expression. The larger, more abundant transcript was expressed at high levels in the germinal compartment of the testis, particularly in germ cells in meiotic prophase. The smaller hsp90 transcript was expressed predominantly in the somatic compartment of the testis. Expression of the two hsp90 transcripts was observed in testes of other species, suggesting an important role for hsp90 in mammalian testicular function. In addition, expression of both hsp90 transcripts was detected in the embryonic and extra-embryonic compartments of mid-gestation embryos.
authors.affiliation: Center for Reproductive Sciences, Columbia University, College of Physicians and Surgeons, New York, New York 10032.
authors.forename: C M
authors.initials: CM
authors.lastname: Gruppi
authors.forename: Z F
authors.initials: ZF
authors.lastname: Zakeri
authors.forename: D J
authors.initials: DJ
authors.lastname: Wolgemuth
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D005314:Embryonic and Fetal Development / Q000235:genetics*; D005786:Gene Expression Regulation; D005854:Germ Cells / Q000166:cytology*; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D008251:Macaca; D008297:Male; D051379:Mice; D005810:Multigene Family; D013091:Spermatogenesis / Q000235:genetics; D013737:Testis / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D006360:Heat-Shock Proteins