Enzyme linked immunosorbent assay (ELISA) for human IgG (Gm) allotype determination.
An inhibition enzyme-linked immunosorbent assay (inhibition-ELISA) was developed for the quantitative determination of human IgG (Gm) allotypes using rabbit anti-Gm antisera, alkaline-phosphatase-conjugated goat anti-rabbit IgG and, as the calibrant, purified human myeloma proteins possessing the relevant Gm allotype. The assay is reproducible and can detect as little as 10 ng/ml of G1m(a), G2m(n) or G3m(st), and 100 ng/ml of G1m(f) or G3m(g). Using this assay, the "gene dosage effect" and "allelic balance" in healthy Japanese were studied.
Ota
T
T
First Department of Internal Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Higashi
S
S
Suzuki
H
H
Eto
S
S
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000918:Antibody Specificity; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005787:Gene Frequency; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007126:Immunoglobulin Allotypes / Q000032:analysis* / Q000235:genetics; D007074:Immunoglobulin G / Q000276:immunology*; D007564:Japan; D011489:Protein Denaturation
D000888:Antibodies, Anti-Idiotypic; D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G