title: Construction and characterization of a single-chain catalytic antibody.
abstract: The antigen-binding (Fab) fragment of the catalytic monoclonal antibody NPN43C9 has recently been cloned by using bacteriophage lambda. By inserting the variable regions of this Fab coding sequence into a (NH2)-VL-linker-VH-(COOH) construct (where VL and VH represent the heavy and light chain variable regions), we have assembled a recombinant gene encoding a catalytic single-chain antigen-binding protein. This protein has been expressed in Escherichia coli and exhibits the same catalytic parameters as the parent monoclonal antibody NPN43C9. Single-chain forms of catalytic antibodies may prove valuable for structural and site-directed mutagenesis studies as well as for large-scale applications of catalytic antibodies.
authors.affiliation: Department of Chemistry, Pennsylvania State University, University Park 16802.
authors.forename: R A
authors.initials: RA
authors.lastname: Gibbs
authors.forename: B A
authors.initials: BA
authors.lastname: Posner
authors.forename: D R
authors.initials: DR
authors.lastname: Filpula
authors.forename: S W
authors.initials: SW
authors.lastname: Dodd
authors.forename: M A
authors.initials: MA
authors.lastname: Finkelman
authors.forename: T K
authors.initials: TK
authors.lastname: Lee
authors.forename: M
authors.initials: M
authors.lastname: Wroble
authors.forename: M
authors.initials: M
authors.lastname: Whitlow
authors.forename: S J
authors.initials: SJ
authors.lastname: Benkovic
mesh_terms: D000595:Amino Acid Sequence; D000906:Antibodies / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D002384:Catalysis; D003001:Cloning, Molecular; D004950:Esterases / Q000235:genetics*; D005822:Genetic Vectors; D007700:Kinetics; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D011994:Recombinant Proteins
chemical_list: D000906:Antibodies; D046911:Macromolecular Substances; D011994:Recombinant Proteins; D004950:Esterases