title: Suppression of lipoprotein lipase in 3T3-L1 cells by a mediator produced by SEKI melanoma, a cachexia-inducing human melanoma cell line.
abstract: Production of a cachexia-inducing factor(s) by the SEKI melanoma cell line, established from a human melanoma, has been well documented. Conditioned medium from cultures of this melanoma cell line contains a factor(s) that inhibits the activity of lipoprotein lipase (LPL) in fully differentiated 3T3-L1 adipocytes. The mode of inhibition of this enzyme by the factor, i.e. its dose-dependency and time course, is very similar to that of LPL-inhibition by a macrophage-derived cachexia-inducing factor, cachectin/tumor necrosis factor (cachectin/TNF). However, the conditioned medium of SEKI melanoma cells does not contain any immuno-reactive substances reactive in enzyme-linked immunosorbent assay (ELISA) with anti-cachectin/TNF antibody, or with anti-interleukin 1 alpha or beta antibodies. This LPL-suppression factor present in the conditioned medium seems to be a peptide because of its heat-lability and apparent molecular weight of more than 25,000. The conditioned media from cultures of four other different cell lines were found to show no significant suppression of LPL activity. These results imply that SEKI melanoma cells produce a cachexia-inducing factor(s) similar to cachectin/TNF but that the molecule involved is different.
authors.affiliation: Clinical Research Institute, National Medical Center Hospital, Tokyo.
authors.forename: M
authors.initials: M
authors.lastname: Kawakami
authors.forename: Y
authors.initials: Y
authors.lastname: Kondo
authors.forename: Y
authors.initials: Y
authors.lastname: Imai
authors.forename: M
authors.initials: M
authors.lastname: Hashiguchi
authors.forename: H
authors.initials: H
authors.lastname: Ogawa
authors.forename: A
authors.initials: A
authors.lastname: Hiragun
authors.forename: S
authors.initials: S
authors.lastname: Aotsuka
authors.forename: S
authors.initials: S
authors.lastname: Shibata
authors.forename: T
authors.initials: T
authors.lastname: Oda
authors.forename: T
authors.initials: T
authors.lastname: Murase
mesh_terms: D000818:Animals; D002100:Cachexia / Q000209:etiology; D002460:Cell Line; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis / Q000494:pharmacology; D008071:Lipoprotein Lipase / Q000037:antagonists & inhibitors*; D008545:Melanoma / Q000150:complications / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000494:pharmacology
chemical_list: D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D008071:Lipoprotein Lipase