title: Cloning and sequence determination of the Schizosaccharomyces pombe rpb1 gene encoding the largest subunit of RNA polymerase II.
abstract: The gene, rpb1, encoding the largest subunit of RNA polymerase II has been cloned from Schizosaccharomyces pombe using the corresponding gene, RPB1, of Saccharomyces cerevisiae as a cross-hybridization probe. We have determined the complete sequence of this gene, and parts of PCR-amplified rpb1 cDNA. The predicted coding sequence, interrupted by six introns, encodes a polypeptide of 1,752 amino acid residues in length with a molecular weight of 194 kilodaltons. This polypeptide contains eight conserved structural domains characteristic of the largest subunit of RNA polymerases from other eukaryotes and, in addition, 29 repetitions of the C-terminal heptapeptide found in all the eukaryotic RNA polymerase II largest subunits so far examined.
authors.affiliation: Department of Molecular Genetics, National Institute of Genetics, Shizuoka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Azuma
authors.forename: M
authors.initials: M
authors.lastname: Yamagishi
authors.forename: R
authors.initials: R
authors.lastname: Ueshima
authors.forename: A
authors.initials: A
authors.lastname: Ishihama
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D012319:RNA Polymerase II / Q000235:genetics*; D012331:RNA, Fungal / Q000235:genetics; D015183:Restriction Mapping; D012568:Schizosaccharomyces / Q000201:enzymology / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D012331:RNA, Fungal; D029702:Schizosaccharomyces pombe Proteins; D012319:RNA Polymerase II; C000606154:Rpb1 protein, S pombe