Contribution of the cell-surface-associated enzyme in the Zimmermann-Rosselet assay of outer membrane permeability of beta-lactam antibiotics.
Analysis of beta-lactam hydrolysis by intact cells of several strains of enteric bacteria suggests that a significant fraction of beta-lactamase molecules is present at a location directly accessible for the substrates in the medium. We propose a method to correct for the error introduced by these "surface-exposed" enzymes in the Zimmermann-Rosselet assay of outer membrane permeability.
Liu
W
W
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Nikaido
H
H
D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics*; D002462:Cell Membrane / Q000201:enzymology; D002463:Cell Membrane Permeability / Q000502:physiology*; D006090:Gram-Negative Bacteria / Q000201:enzymology / Q000648:ultrastructure; D007700:Kinetics; D001618:beta-Lactamases / Q000378:metabolism*; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases