Multipoint linkage analysis of DXS369 and DXS304 in fragile X families.
Diagnosis of carriers of the fragile-X mental retardation gene is hampered by the paucity of tightly linked DNA markers. Recently, 2 new DNA markers RN1 (DXS369) and U6.2 (DXS304) have become available. Both markers are tightly linked to the fragile-X locus, but their location relative to the fragile site was not known with certainty. We have tested these new markers in a multipoint linkage analysis of 26 fragile-X families typed for DXS105 as a proximal marker and DXS52 as a distal marker. Our results establish the order DXS105-DXS369-fra(X)-DXS304-DXS52, which is in agreement with physical mapping results.
van Oost
B A
BA
Department of Human Genetics, University Hospital Nijmegen, The Netherlands.
Smits
A
A
Dreesen
J C
JC
Smeets
D
D
Perdon
L
L
van Bennekom
C A
CA
Dahl
N
N
Bakker
E
E
Oostra
B A
BA
D004247:DNA / Q000032:analysis; D015342:DNA Probes; D005260:Female; D005600:Fragile X Syndrome / Q000175:diagnosis / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D008126:Lod Score; D008297:Male; D011995:Recombination, Genetic
D015342:DNA Probes; D005819:Genetic Markers; D004247:DNA