title: The beta chain of chicken fibrinogen contains an atypical thrombin cleavage site.
abstract: A cDNA corresponding to almost the entire coding region of the mRNA for the beta chain of chicken fibrinogen was sequenced. At the protein level, significant homology to the beta subunits of other vertebrate fibrinogens was found, with the highest degree of amino acid identity localized in the C-terminal region. In general, features conserved in the fibrinogens from other species also characterize the chicken sequence, including the cysteine motifs bordering an alpha-helical permissive region of fixed length and a single glycosylation site in the C-terminal region. However, the site of thrombin-catalyzed cleavage, which in other species consists of an Arg-Gly peptide bond, is instead an Arg-Ala bond in the chicken beta chain. The Ala was confirmed directly from a sequencing analysis of the purified beta chain of chicken fibrin. This finding may explain the observed slow clotting time of chicken fibrinogen relative to that of other species.
authors.affiliation: Lindsley F. Kimball Research Institute of the New York Blood Center, New York 10021.
authors.forename: L
authors.initials: L
authors.lastname: Weissbach
authors.forename: C
authors.initials: C
authors.lastname: Oddoux
authors.forename: R
authors.initials: R
authors.lastname: Procyk
authors.forename: G
authors.initials: G
authors.lastname: Grieninger
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002645:Chickens; D005340:Fibrinogen / Q000235:genetics* / Q000378:metabolism; D005345:Fibrinopeptide B / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007798:Lampreys; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D013917:Thrombin / Q000378:metabolism*
chemical_list: D046911:Macromolecular Substances; D005345:Fibrinopeptide B; D005340:Fibrinogen; D013917:Thrombin