Stimulation-produced analgesia in the mouse: evidence for laterality of opioid mediation.
The effect of naloxone on periaqueductal gray matter (PAG) stimulation-produced analgesia (SPA) was examined in pentobarbital anesthetized Swiss-Webster mice. Electrodes were placed either in dorsolateral or ventrolateral PAG, and SPA threshold was assessed using the hind paw-flick test (paw withdrawal from radiant heat). SPA threshold did not differ between dorsal and ventral PAG, and naloxone equally attenuated SPA from both areas. SPA threshold for the paw contralateral to the stimulation site was half that for the ipsilateral paw. Elevation of SPA threshold by naloxone was greater for the contralateral than ipsilateral paw. Exposure to analgesic neck pinch prior to SPA almost completely abolished the antinociceptive effect of contralateral PAG stimulation without affecting SPA on the ipsilateral paw. This effect of pinch was itself reversed by prior naloxone administration. We suggest that the substrate of opioid mediated SPA from PAG in the mouse has principally a contralateral organization.
Marek
P
P
Department of Psychology, University of California, Los Angeles 90024.
Yirmiya
R
R
Liebeskind
J C
JC
D000698:Analgesia; D000818:Animals; D004558:Electric Stimulation; D004723:Endorphins / Q000502:physiology*; D007839:Functional Laterality; D051379:Mice; D008815:Mice, Inbred Strains; D010147:Pain Measurement; D010487:Periaqueductal Gray / Q000502:physiology*; D012684:Sensory Thresholds
D004723:Endorphins