Subtypes of protein kinase C in rat cerebral microvessels.
Protein kinase C in rat cerebral microvessels was characterized. By hydroxyapatite column chromatography, protein kinase C in the soluble fraction was resolved into two major peaks corresponding to type II and III enzymes, in the proportion of 57% and 38%, respectively. Since each subtype is considered to have a distinct role, the high proportion of type II enzyme found in this study suggests that this type may be involved in specific functions of the cerebral microvessels.
Kobayashi
H
H
Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, Kitakyushu, Japan.
Mizuki
T
T
Koda
Y
Y
Okazaki
M
M
Kuroiwa
A
A
Izumi
F
F
D000818:Animals; D001808:Blood Vessels / Q000201:enzymology*; D001921:Brain / Q000098:blood supply*; D002118:Calcium / Q000378:metabolism; D002846:Chromatography, Affinity; D007527:Isoenzymes / Q000032:analysis*; D008297:Male; D011493:Protein Kinase C / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D007527:Isoenzymes; D011493:Protein Kinase C; D002118:Calcium