Estimation of allele frequencies for VNTR loci.
VNTR loci provide valuable information for a number of fields of study involving human genetics, ranging from forensics (DNA fingerprinting and paternity testing) to linkage analysis and population genetics. Alleles of a VNTR locus are simply fragments obtained from a particular portion of the DNA molecule and are defined in terms of their length. The essential element of a VNTR fragment is the repeat, which is a short sequence of basepairs. The core of the fragment is composed of a variable number of identical repeats that are linked in tandem. A sample of fragments from a population of individuals exhibits substantial variation in length because of variation in the number of repeats. Each distinct fragment length defines an allele, but any given fragment is measured with error. Therefore the observed distribution of fragment lengths is not discrete but is continuous, and determination of distinct allele classes is not straightforward. A mixture model is the natural statistical method for estimating the allele frequencies of VNTR loci. In this article we develop nonparametric methods for obtaining the distribution of allele sizes and estimates of their frequencies. Methods for obtaining maximum-likelihood estimates are developed. In addition, we suggest an empirical Bayes method to improve the maximum-likelihood estimates of the gene frequencies; the empirical Bayes procedure effects a local smoothing. The latter method works particularly well when measurement error is large relative to the repeat size, because the estimated distribution of allele frequencies when maximum likelihood is used is unreliable because of an alternating pattern of over- and underestimation. We define alleles and estimate the allele frequencies for two VNTR loci from the human genome (D17S79 and D2S44), from data obtained from Lifecodes, Inc.
Devlin
B
B
Department of Epidemiology and Public Health, Yale University, New Haven, CT 06510.
Risch
N
N
Roeder
K
K
D000483:Alleles; D001499:Bayes Theorem; D002874:Chromosome Mapping; D004247:DNA / Q000737:chemistry*; D005787:Gene Frequency; D006801:Humans; D008957:Models, Genetic; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid
D004247:DNA