The effects of interleukins 4 and 5 on the differentiation of B cells from (NZB x NZW)F1 mice.
We describe here that IL-4 and IL-5 together can induce the production of high amounts of IgG1 polyclonal antibodies by B cells from old (NZB x NZW)F1 mice even in the absence of LPS. The effect was less marked in young mice from the same strain or in normal Balb/c mice. The cells sensitive to the treatment with the interleukins were the large B cells. Old mice had higher proportions of such cells than young mice or normal Balb/c mice. Synthesis of anti-DNA antibodies of the IgM class was induced. Although IL-4 plus IL-5 induced a strong polyclonal IgG1 production, there were no IgG1 anti-DNA antibodies.
Alarcón-Riquelme
M E
ME
Department of Immunology, Arrhenius Laboratories for Natural Sciences, Stockholm University, Sweden.
Möller
G
G
Fernández
C
C
D000818:Animals; D000974:Antibodies, Antinuclear / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000276:immunology; D015847:Interleukin-4 / Q000494:pharmacology*; D015848:Interleukin-5 / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008814:Mice, Inbred NZB
D000974:Antibodies, Antinuclear; D007074:Immunoglobulin G; D015848:Interleukin-5; D015847:Interleukin-4