title: Isolation and nucleotide sequence of the Aspergillus restrictus gene coding for the ribonucleolytic toxin restrictocin and its expression in Aspergillus nidulans: the leader sequence protects producing strains from suicide.
abstract: We describe the cloning and characterization of the gene coding for the ribotoxin restrictocin, from Aspergillus restrictus (gene res, EMBL accession Number X56176). This toxin is a potent inhibitor of protein synthesis in eucaryotes and is of potential interest as a component of immunotoxins. To analyze the mechanism of self-protection in the producing organism, the res gene was cloned into the vector pFB39 and introduced into Aspergillus nidulans. The secretion of active restrictocin from transformants suggests that the pro-toxin is not an active nuclease but is activated during the process of secretion.
authors.affiliation: Unite de genie microbiologique, Institut Pasteur, Paris, France.
authors.forename: B
authors.initials: B
authors.lastname: Lamy
authors.forename: J
authors.initials: J
authors.lastname: Davies
mesh_terms: D000485:Allergens; D000595:Amino Acid Sequence; D000818:Animals; D052179:Antigens, Plant; D001230:Aspergillus / Q000235:genetics*; D001233:Aspergillus nidulans / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004271:DNA, Fungal; D005656:Fungal Proteins / Q000235:genetics*; D015870:Gene Expression; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D011500:Protein Synthesis Inhibitors; D011817:Rabbits; D012045:Regulatory Sequences, Nucleic Acid; D012260:Ribonucleases; D014170:Transformation, Genetic
chemical_list: C081947:ASPF1 protein, Aspergillus fumigatus; D000485:Allergens; D052179:Antigens, Plant; D004271:DNA, Fungal; D005656:Fungal Proteins; D011500:Protein Synthesis Inhibitors; C036196:MITF protein, Aspergillus restrictus; D012260:Ribonucleases