title: Eukaryotic protein synthesis initiation factor 2. A target for inactivation by proanthocyanidin.
abstract: Polyproanthocyanidin (PPA), a phenolic polymer isolated from the plant Alhagi kirgisorum S. was found to interact strongly with eukaryotic initiation factor 2 (eIF-2), thereby inhibiting reactions involving this protein. When added to a rabbit reticulocyte lysate system, PPA blocks in vitro translation and it appears to selectively bind and precipitate a relatively small number of proteins including eIF-2 and regulin. The phosphorylation of purified regulin and eIF-2 by casein kinase II (CK II) and the heme-sensitive eIF-2 alpha kinase, respectively, was also inhibited by the polyphenolic compound. The natural fluorescence of PPA was utilized to compare its interaction with eIF-2 and regulin to that with other natural and synthetic polypeptides.
authors.affiliation: Department of Chemistry and Biochemistry, University of Texas, Austin 78712.
authors.forename: W
authors.initials: W
authors.lastname: Kudlicki
authors.forename: W D
authors.initials: WD
authors.lastname: Picking
authors.forename: G
authors.initials: G
authors.lastname: Kramer
authors.forename: B
authors.initials: B
authors.lastname: Hardesty
authors.forename: S K
authors.initials: SK
authors.lastname: Smailov
authors.forename: B G
authors.initials: BG
authors.lastname: Mukhamedzhanov
authors.forename: A V
authors.initials: AV
authors.lastname: Lee
authors.forename: B K
authors.initials: BK
authors.lastname: Iskakov
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000494:pharmacology*; D047388:Casein Kinases; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D015852:Eukaryotic Initiation Factor-2 / Q000378:metabolism*; D010766:Phosphorylation; D044945:Proanthocyanidins; D014176:Protein Biosynthesis; D011494:Protein Kinases / Q000494:pharmacology; D011817:Rabbits
chemical_list: D000872:Anthocyanins; D003598:Cytoskeletal Proteins; D015852:Eukaryotic Initiation Factor-2; D044945:Proanthocyanidins; C013221:proanthocyanidin; D011494:Protein Kinases; D047388:Casein Kinases