title: Fibroblast growth factor-mediated proliferation of central nervous system precursors depends on endogenous production of insulin-like growth factor I.
abstract: Fibroblast growth factor stimulates proliferation and subsequent differentiation of precursor cells isolated from the neuroepithelium of embryonic day 10 mice in vitro. Here we show that fibroblast growth factor-induced proliferation is dependent on the presence of insulin-like growth factors (IGFs) and that IGF-I is endogenously produced by the neuroepithelial cells. Blocking of endogenous IGF-I activity with anti-IGF-I antibodies results in complete inhibition of fibroblast growth factor-mediated proliferation and in cell death. IGF-I alone acts as a survival agent. These observations correlate with the detection of transcripts for IGF-I and basic fibroblast growth factor in freshly isolated neuroepithelium and are consistent with an autocrine action of these factors in early brain development in vivo.
authors.affiliation: Walter and Eliza Hall Institute of Medical Research, Royal Parade, Parkville, Victoria, Australia.
authors.forename: J
authors.initials: J
authors.lastname: Drago
authors.forename: M
authors.initials: M
authors.lastname: Murphy
authors.forename: S M
authors.initials: SM
authors.lastname: Carroll
authors.forename: R P
authors.initials: RP
authors.lastname: Harvey
authors.forename: P F
authors.initials: PF
authors.lastname: Bartlett
mesh_terms: D000818:Animals; D000906:Antibodies; D001345:Autoradiography; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects*; D004622:Embryo, Mammalian; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis / Q000276:immunology / Q000502:physiology*; D007700:Kinetics; D008636:Mesencephalon / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008808:Mice, Inbred CBA; D011994:Recombinant Proteins / Q000494:pharmacology; D013687:Telencephalon / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
chemical_list: D000906:Antibodies; D011994:Recombinant Proteins; D014316:Tritium; D016222:Fibroblast Growth Factor 2; D007334:Insulin-Like Growth Factor I; D013936:Thymidine