Construction, arraying, and high-density screening of large insert libraries of human chromosomes X and 21: their potential use as reference libraries.
We have constructed cosmid libraries from flow-sorted human chromosomes X and 21, each of which contains greater than 30 genome equivalents, and have developed systems allowing permanent storage of primary clones, easy screening of libraries in high-density filter formats, and the simultaneous generation of fingerprinting and mapping data on the same set of cosmid clones. Clones are picked into microtiter plate wells and stored at -70 degrees C. A semiautomatic robot system allows the generation of filter replicas containing up to 10,000 clones per membrane. Sets of membranes containing 15-20 chromosome equivalents of both chromosomes will be used for the construction of ordered clone libraries by hybridization fingerprinting protocols. In addition, multiple sets of two membranes containing 4 chromosome equivalents of the human X chromosome, and one membrane containing 3 chromosome equivalents of chromosome 21, have been distributed to other interested laboratories as part of a system of reference libraries. This system allows other groups easy access to the clones and offers an efficient protocol to combine results generated in different laboratories using these libraries. Here we describe the construction of the libraries and demonstrate the use of high-density screening filters in oligonucleotide probe hybridizations and the isolation of cosmids by hybridization with probes from the X chromosome.
Nizetić
D
D
Genome Analysis Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
Zehetner
G
G
Monaco
A P
AP
Gellen
L
L
Young
B D
BD
Lehrach
H
H
D002891:Chromosomes, Human, Pair 21; D003360:Cosmids; D016172:DNA Fingerprinting; D015342:DNA Probes; D005434:Flow Cytometry; D015698:Genomic Library; D006801:Humans; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D014960:X Chromosome
D015342:DNA Probes; D015345:Oligonucleotide Probes