title: Motile sperm recovery from fresh and frozen-thawed ejaculates using a swim-up procedure.
abstract: The recovery of motile sperm in swim-ups from fresh semen that was washed and centrifuged one time was 33% and after 2 washes and centrifugations was 22%. The recovery rates for frozen-thawed semen were only 7% and 5%, respectively. The straight line velocity of sperm was increased after sperm swim-up, whereas changes to the lateral sperm head movement were not as evident. However, the dramatic loss in motile sperm numbers especially after swim-up from thawed semen indicate that more efficient methods of isolating motile sperm from thawed semen may be required.
authors.affiliation: Department of Obstetrics and Gynecology, University of Southern California-Los Angeles.
authors.forename: J W
authors.initials: JW
authors.lastname: Graczykowski
authors.forename: M S
authors.initials: MS
authors.lastname: Siegel
mesh_terms: D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology; D006801:Humans; D008297:Male; D012661:Semen; D013048:Specimen Handling / Q000379:methods*; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000187:drug effects
chemical_list: D003451:Cryoprotective Agents