Expression of cytoskeletal components in melanocytic skin lesions. An immunohistochemical study.
The cytoskeleton is considered to be important for maintaining cell shape and facilitating cell movement. In the present study, the expression of cytoskeletal components is examined in benign and malignant melanocytic skin tumors. Paraffin sections of 75 cases (25 each of nevocellular nevus, primary malignant melanoma, and cutaneous metastases of malignant melanoma) were stained with antibodies to tubulin, myosin, actin, and vimentin using a three-step immunoperoxidase method. The staining results were assessed independently for tumor cells and stroma cells in comparison to inbuilt reference structures. Vimentin is found in all melanocytic lesions in the tumor as well as in the stroma cells. In malignant lesions, the tumor cell staining intensity varies between neighboring regions; particularly in malignant melanoma the staining is pronounced in the tumor periphery (chi 2 test: p less than 0.05). Actin is only weakly positive in nevus cells and primary melanoma tumor cells, but strongly expressed in metastatic tumor cells (p less than 0.001). Nevus fibroblasts are only weakly positive, whereas the stroma fibroblasts in the malignant lesions are strongly positive (p less than 0.001). The same is true for myosin and tubulin expression in dermal fibroblasts (p less than 0.001), whereas the tumor cells are equally (weakly) positive in all melanocytic lesions. Our study shows that there are significant differences in the immunohistochemical expression of cytoskeletal components in various melanocytic tumors. There is an elevated expression of vimentin and actin in the tumor cells, particularly of metastatic lesions. However, the most pronounced differences are found in the dermal fibroblasts.(ABSTRACT TRUNCATED AT 250 WORDS)
Puches
R
R
Department of Dermatology, University of Graz, Austria.
Smolle
J
J
Rieger
E
E
Soyer
H P
HP
Kerl
H
H
D000199:Actins / Q000032:analysis; D003598:Cytoskeletal Proteins / Q000032:analysis*; D003599:Cytoskeleton / Q000737:chemistry*; D005347:Fibroblasts / Q000737:chemistry / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D008544:Melanocytes / Q000737:chemistry / Q000648:ultrastructure; D008545:Melanoma / Q000737:chemistry* / Q000556:secondary / Q000648:ultrastructure; D009218:Myosins / Q000032:analysis; D009508:Nevus, Pigmented / Q000737:chemistry* / Q000648:ultrastructure; D012878:Skin Neoplasms / Q000737:chemistry* / Q000556:secondary / Q000648:ultrastructure; D014404:Tubulin / Q000032:analysis; D014746:Vimentin / Q000032:analysis
D000199:Actins; D003598:Cytoskeletal Proteins; D014404:Tubulin; D014746:Vimentin; D009218:Myosins