Variations of plasma sialic acid and N-acetylglucosamine levels in cancer, inflammatory diseases and bone marrow transplantation: a proton NMR spectroscopy study.
Proton NMR spectroscopy allows the detection in plasma of resonances arising from N-acetyl-glucosamine (NAG) and N-acetyl-neuraminic acid (NANA) which have been shown to be borne by acute phase glycoproteins. These resonances can be identified using 2 different protocols of spectrum acquisition detecting different physical states in the global pool of glycoproteins, ie mobile and less mobile moieties of glycosylated chains. In this study we demonstrate that NMR spectroscopy allows a precise monitoring of the variations of glycosylated residues in cancers, inflammatory processes and bone marrow transplantation. The most important findings are that: i), the distribution of glycosylated residues varies with the origin of the cancerous tissue; ii), the level of these residues is a function of tumor development; iii), the concentrations in NAG and NANA are well correlated with the standard biological parameters of acute phase and leucocyte activation. Proton NMR spectroscopy of glycosylated residues in plasma may offer a new means of monitoring sialic acid in cancer and other pathological conditions.
Kriat
M
M
Centre de Résonance Magnétique Biologique et Médicale, URA CNRS 1186, Faculté Médecine, Marseille, France.
Vion-Dury
J
J
Fayre
R
R
Maraninchi
D
D
Harlé
J R
JR
Confort-Gouny
S
S
Sciaky
M
M
Fontanarava
E
E
Viout
P
P
Cozzone
P J
PJ
D000117:Acetylglucosamine / Q000097:blood*; D000409:Alanine / Q000097:blood; D016026:Bone Marrow Transplantation; D006801:Humans; D007249:Inflammation / Q000097:blood*; D008137:Longitudinal Studies; D009682:Magnetic Resonance Spectroscopy; D019158:N-Acetylneuraminic Acid; D009369:Neoplasms / Q000097:blood*; D012794:Sialic Acids / Q000097:blood*; D013997:Time Factors
D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid; D000409:Alanine; D000117:Acetylglucosamine