Isolation of IgG1-secreting switch variants from IgM hybridomas produced after in vitro immunization.
IgG1-secreting variants have been isolated from three different IgM-secreting hybridomas, in two instances following in vitro immunization. The method used was based on sequential sublining in combination with selection by an IgG1-specific two-site ELISA system employing two different IgG1-specific polyclonal antisera. Idiotypic identity between the IgG1 variants and their respective IgM parent was demonstrated using syngeneic anti-idiotypic antisera. The antigen binding specificity in the IgG1 variants was also conserved. Isolation of naturally occurring IgG1 switch variants from IgM-secreting hybridomas that are produced after in vivo immunization offers a solution to the major disadvantages associated with the generation of IgM hybridomas.
Mercken
M
M
Innogenetics, Ghent, Belgium.
Vandermeeren
M
M
Lubke
U
U
Boons
J
J
Van de Voorde
A
A
Gheuens
J
J
D000544:Alzheimer Disease / Q000378:metabolism; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D001921:Brain / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology / Q000302:isolation & purification*; D007075:Immunoglobulin M / Q000276:immunology*; D007134:Immunoglobulin Switch Region; D051379:Mice; D008807:Mice, Inbred BALB C; D009454:Neurofibrils / Q000378:metabolism
D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D007075:Immunoglobulin M