Particle-enhanced turbidimetric immunoassay of sex-hormone-binding globulin in serum.
A particle-enhanced turbidimetric immunoassay (PETIA) for human sex-hormone-binding globulin (SHBG) is described. The method involves use of antibody covalently coupled to latex particles and is almost fully automated, with sample processing being complete in less than 20 min. The working reagents are stable for at least three months, and full calibration of the assay each day is not essential. A particular advantage is that pretreatment of samples is rarely required because the working range of the assay is from 2.0 to 320 nmol/L for nondiluted serum. Intra- and interassay CVs were less than 4.5% and 8.5%, respectively, and mean analytical recovery was 101.5%. SHBG concentrations of 129 serum samples determined by this method and by a commercially available immunoradiometric assay correlated highly.
Deleo
D T
DT
School of Biomedical Sciences, Curtin University of Technology, Bentley, Western Australia.
Lee
I R
IR
Wetherall
J D
JD
Newman
D J
DJ
Medcalf
E A
EA
Price
C P
CP
D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods; D015592:Immunoradiometric Assay / Q000379:methods; D007840:Latex; D008297:Male; D009391:Nephelometry and Turbidimetry / Q000379:methods; D011247:Pregnancy; D012738:Sex Hormone-Binding Globulin / Q000032:analysis*
D007840:Latex; D012738:Sex Hormone-Binding Globulin