Gene conversion in Drosophila and the effects of the meiotic mutants mei-9 and mei-218.
Simple meiotic gene conversion tracts produced in wild-type females were compared with those from two meiotic mutants, mei-9 and mei-218. The positions and lengths of conversion tracts were determined by denaturing gradient gels and DNA sequencing. Conversion tracts in wild type averaged 885 base pairs in length, were continuous, and displayed no obvious hot spots of initiation. Some unusual conversion events were found in the mei-218 and mei-9 samples, although most events were indistinguishable from wild-type tracts in their length and continuity.
Curtis
D
D
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115.
Bender
W
W
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D003434:Crossing Over, Genetic; D004330:Drosophila / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005785:Gene Conversion; D005819:Genetic Markers; D008540:Meiosis / Q000235:genetics*; D009030:Mosaicism; D009154:Mutation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011995:Recombination, Genetic; D015183:Restriction Mapping
D005819:Genetic Markers