Nucleotide sequence of tomato aspermy virus RNA 2.
RNA 2 of the V strain of tomato aspermy virus (TAV) consists of 3074 nucleotides and contains one open reading frame of 2487 nucleotides. Thus, it resembles RNA 2 of cucumber mosaic virus (CMV) strains Q and Fny (62% identical to both), brome mosaic virus (42% identical) and cowpea chlorotic mottle virus (40% identical). In comparisons between amino acid sequences, three different regions of similarity could be distinguished. These were the central part (amino acids 224 to 757 for V-TAV), which was most similar among the four viruses, and the N and C ends; sequences conserved among RNA polymerase species were found in the C half of the central part. Hydrophobicity patterns, and distributions of acidic and basic amino acids in the proteins encoded by V-TAV RNA 2, Q-CMV RNA 2 and Fny-CMV RNA 2 were very similar except at the extreme ends of the molecules. Structures that have been reported to act as regulatory signals for minus- and plus-strand synthesis were found in the 5' and 3' non-coding regions of the RNA.
Moriones
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Departamento de Patología Vegetal, ETSI Agrónomos, Ciudad Universitaria, Madrid, Spain.
Roossinck
M J
MJ
García-Arenal
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F
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005814:Genes, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010942:Plant Viruses / Q000201:enzymology / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics
D003062:Codon; D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases