title: Nerve growth factor promotes CNS cholinergic axonal regeneration into acellular peripheral nerve grafts.
abstract: Peripheral nerve grafts promote vigorous regeneration of adult mammalian CNS axons. Elimination of nerve-associated cells by freeze-thawing abolishes this promoting quality, possibly by creating inhibitory cellular debris and/or destroying the production of stimulatory factors by living Schwann or other cells. Here, debris-free acellular peripheral nerve segments placed between the disconnected septum and the hippocampal formation acquired almost no cholinergic axons after 1 month. However, such acellular nerve grafts treated before implantation with purified beta-nerve growth factor (NGF) contained nearly as many longitudinally oriented cholinergic axons as did fresh cellular nerve grafts. These results suggest that (i) NGF is required for the regeneration of adult CNS cholinergic axons into nerve grafts and (ii) an important function of living cells within peripheral nerve may be the production of neuronotrophic factors such as NGF.
authors.affiliation: Department of Biology, University of California, San Diego, La Jolla 92093.
authors.forename: T
authors.initials: T
authors.lastname: Hagg
authors.forename: A K
authors.initials: AK
authors.lastname: Gulati
authors.forename: M A
authors.initials: MA
authors.lastname: Behzadian
authors.forename: H L
authors.initials: HL
authors.lastname: Vahlsing
authors.forename: S
authors.initials: S
authors.lastname: Varon
authors.forename: M
authors.initials: M
authors.lastname: Manthorpe
mesh_terms: D000110:Acetylcholinesterase / Q000032:analysis*; D000818:Animals; D001369:Axons / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D005260:Female; D006624:Hippocampus / Q000502:physiology / Q000648:ultrastructure; D008854:Microscopy, Electron; D009412:Nerve Fibers / Q000648:ultrastructure; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009416:Nerve Regeneration / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D012584:Sciatic Nerve / Q000502:physiology / Q000637:transplantation* / Q000648:ultrastructure; D013997:Time Factors; D016042:Transplantation, Heterotopic
chemical_list: D009414:Nerve Growth Factors; D000110:Acetylcholinesterase