title: Current status of bioassay procedures to detect and quantify previous exposures to radioactive materials. Bioassay Procedures Working Group.
abstract: This report was prepared by a working group established by the Oak Ridge Associated Universities (ORAU) for the purpose of assessing the current capabilities of bioassay methods that can be used to determine the occurrence and magnitude of a previous internal deposition of one or more radionuclides. The first five sections discuss general features of the use of in-vitro bioassay samples to achieve this purpose. The remainder of the report is focused on the possible use of urine bioassay procedures to detect and quantify internal depositions of radionuclides that may have occurred in United States occupation troops in Hiroshima or Nagasaki, Japan, prior to 1 July 1946, or to personnel who participated in atmospheric nuclear weapons tests conducted between 1945 and 1962. Theoretical calculations were made to estimate the quantities of various radionuclides produced in a 20-kiloton (kt) nuclear detonation that might still be present in measurable quantities in people today if they were exposed 25 to 40 y ago. Two radionuclides that emerged as good choices for this type of bioassay analysis were 90Sr, which emits beta particles, and 239,240Pu, which emits alpha particles. The current status and future prospects of chemical procedures for analyzing in-vitro urine bioassay samples for these two radionuclides were examined to determine the minimum amounts that could be detected with current methods and how much one might expect the sensitivity of detection to improve in the near future. Most routine 239,240Pu bioassay analyses involve detection by alpha spectrometry. The current minimum detectable amount (MDA) is about 0.74 mBq L-1 (20 fCi L-1), but this could be lowered to 74 muBq L-1 (2 fCi L-1). An MDA of 0.74 mBq L-1 (20 fCi L-1) is adequate for routine bioassay analyses but is too high to detect most uptakes of 239,240Pu that may have occurred 25 to 40 y ago. Methods under development that are or can be much more sensitive and have lower MDAs than alpha spectrometry for 239Pu are fission track analysis and mass spectrometry. Currently, the fission track analysis method has an MDA of about 19 muBq L-1), and this may eventually be lowered to 1.9 muBq L-1 (0.005 fCi L-1). The current MDA for 239Pu by mass spectrometry is about 7.4 mBq L-1 (200 fCi L-1), but the potential exists that it could be lowered to a value of about 0.37 muBq L-1 (0.01 fCi L-1).(ABSTRACT TRUNCATED AT 400 WORDS)
authors.affiliation: Lovelace Inhalation Toxicology Research Institute, Albuquerque, NM 87185.
authors.forename: B
authors.initials: B
authors.lastname: Boecker
authors.forename: R
authors.initials: R
authors.lastname: Hall
authors.forename: K
authors.initials: K
authors.lastname: Inn
authors.forename: J
authors.initials: J
authors.lastname: Lawrence
authors.forename: P
authors.initials: P
authors.lastname: Ziemer
authors.forename: G
authors.initials: G
authors.lastname: Eisele
authors.forename: B
authors.initials: B
authors.lastname: Wachholz
authors.forename: W
authors.initials: W
authors.lastname: Burr
mesh_terms: D005243:Feces / Q000737:chemistry; D006801:Humans; D009689:Nuclear Warfare; D016273:Occupational Exposure; D011829:Radiation Dosage; D011847:Radioactive Fallout; D011868:Radioisotopes / Q000032:analysis* / Q000652:urine; D011874:Radiometry / Q000379:methods*; D014018:Tissue Distribution
chemical_list: D011847:Radioactive Fallout; D011868:Radioisotopes