Distribution of endotracheally instilled surfactant protein SP-C in lung-lavaged rabbits.
In lung-lavaged surfactant-deficient rabbits (n = 6) requiring artificial ventilation, porcine surfactant was instilled endotracheally. This resulted in improvement of lung function so that the animals could be weaned off artificial ventilation. The animals were killed 4 1/2 h after surfactant administration and the porcine surfactant protein was localized in the lung with a MAb. We found surfactant protein in all lobes of the lung but the distribution was not homogeneous. Surfactant protein C was found in less than 15% of the alveolar spaces and in less than 1% of the bronchi.
Oetomo
S B
SB
Department of Pediatrics, State University Groningen, The Netherlands.
de Ley
L
L
Curstedt
T
T
ter Haar
J G
JG
Schoots
C
C
Wildevuur
C R
CR
Okken
A
A
D000818:Animals; D008168:Lung / Q000378:metabolism*; D011510:Proteolipids / Q000008:administration & dosage / Q000493:pharmacokinetics*; D011663:Pulmonary Surfactants / Q000008:administration & dosage / Q000493:pharmacokinetics*; D011817:Rabbits; D012121:Respiration, Artificial; D007507:Therapeutic Irrigation; D014018:Tissue Distribution
D011510:Proteolipids; D011663:Pulmonary Surfactants