title: Responses of the L5178Y mouse lymphoma forward mutation assay: V. Gases and vapors.
abstract: A new protocol for testing vapors and gases in the L5178Y mouse lymphoma assay is presented. Four chemicals, propylene, 1,2-propylene oxide, 1,3-butadiene, and vinylidene chloride, were tested for their mutagenic potential. Cultures were exposed to the chemicals, which were delivered as vapors or gases, for 4 hr, then cultured for 2 days before plating in soft agar with or without trifluorothymidine (TFT), 3 microgram/ml. Each chemical was tested at least twice. Significant responses were obtained with 1,2-propylene oxide and vinylidene chloride, but neither cytotoxicity nor mutagenicity was induced by 1,3-butadiene; propylene could not be classified as either mutagenic or non-mutagenic in the assay. Rat liver S9 mix was not a requirement for the mutagenic activity of 1,2-propylene oxide, whereas the liver preparation markedly enhanced both the cytotoxicity and mutagenicity of vinylidene chloride.
authors.affiliation: Inveresk Research International, Ltd., Musselburgh, Scotland.
authors.forename: D
authors.initials: D
authors.lastname: McGregor
authors.forename: A G
authors.initials: AG
authors.lastname: Brown
authors.forename: P
authors.initials: P
authors.lastname: Cattanach
authors.forename: I
authors.initials: I
authors.lastname: Edwards
authors.forename: D
authors.initials: D
authors.lastname: McBride
authors.forename: C
authors.initials: C
authors.lastname: Riach
authors.forename: W
authors.initials: W
authors.lastname: Shepherd
authors.forename: W J
authors.initials: WJ
authors.lastname: Caspary
mesh_terms: D000393:Air Pollutants / Q000633:toxicity; D000475:Alkenes / Q000633:toxicity; D000818:Animals; D002070:Butadienes / Q000633:toxicity; D004000:Dichloroethylenes / Q000633:toxicity; D004852:Epoxy Compounds / Q000633:toxicity; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D008223:Lymphoma / Q000473:pathology; D008741:Methyl Methanesulfonate / Q000633:toxicity; D008748:Methylcholanthrene / Q000633:toxicity; D051379:Mice; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens; D013937:Thymidine Kinase / Q000235:genetics; D014407:Tumor Cells, Cultured / Q000187:drug effects*
chemical_list: D000393:Air Pollutants; D000475:Alkenes; D002070:Butadienes; D004000:Dichloroethylenes; D004852:Epoxy Compounds; D009153:Mutagens; C029297:vinylidene chloride; D008748:Methylcholanthrene; D005020:Ethyl Methanesulfonate; D008741:Methyl Methanesulfonate; C013658:propylene; D013937:Thymidine Kinase; C031763:1,3-butadiene; C009068:propylene oxide