title: Ultraviolet irradiation inhibits RNA decay and modifies ribosomal RNA processing in Trypanosoma brucei.
abstract: A known effect of ultraviolet radiation on transcription is the arrest of RNA elongation. In Trypanosoma brucei, we show that UV also inhibits RNA decay, leading to specific accumulation of transcripts from the beginning of several transcription units. In addition, UV irradiation changes the pattern of ribosomal RNA processing, probably by altering the order in which the non-coding spacers are excised. These effects are still observed on RNA synthesized more than 2 h after irradiation, and do not depend on protein synthesis.
authors.affiliation: Department of Molecular Biology, University of Brussels, Rhode-St.-Genèse, Belgium.
authors.forename: H
authors.initials: H
authors.lastname: Coquelet
authors.forename: M
authors.initials: M
authors.lastname: Steinert
authors.forename: E
authors.initials: E
authors.lastname: Pays
mesh_terms: D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D008562:Membrane Glycoproteins; D011401:Promoter Regions, Genetic; D015800:Protozoan Proteins; D012323:RNA Processing, Post-Transcriptional / Q000528:radiation effects*; D016053:RNA, Protozoan / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism*; D014158:Transcription, Genetic / Q000528:radiation effects; D014346:Trypanosoma brucei brucei / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000235:genetics
chemical_list: D008562:Membrane Glycoproteins; D015800:Protozoan Proteins; D016053:RNA, Protozoan; D012335:RNA, Ribosomal; D014643:Variant Surface Glycoproteins, Trypanosoma; C053277:procyclic acidic repetitive protein, Trypanosoma; D003513:Cycloheximide