title: Characterization of human deoxycytidine kinase. Correlation with cDNA sequences.
abstract: Existing data on the structure of human deoxycytidine kinase (dCK) diverge. A monomeric 60 kDa form has been isolated and the cloning of a cDNA coding for 626 amino acids corresponding to a 71 kDa protein has been reported. However, pure dCK isolated from leukemic spleen is a dimer of 30 kDa subunits. Amino acid sequences of peptides from digests of this protein are now presented. None of the peptide structures obtained correspond to the cDNA for the 71 kDa protein, but to a cDNA for a 30.5 kDa dCK recently cloned. Furthermore, homology of the peptide sequences od dCK to parts of thymidine kinases and protein-tyrosine kinases are detected.
authors.affiliation: Medical Nobel Institute, Department of Biochemistry, Karolinska Institutet, Stockholm, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Eriksson
authors.forename: E
authors.initials: E
authors.lastname: Cederlund
authors.forename: T
authors.initials: T
authors.lastname: Bergman
authors.forename: H
authors.initials: H
authors.lastname: Jörnvall
authors.forename: C
authors.initials: C
authors.lastname: Bohman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry*; D003842:Deoxycytidine Kinase / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011505:Protein-Tyrosine Kinases / Q000737:chemistry / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013154:Spleen / Q000737:chemistry; D013937:Thymidine Kinase / Q000737:chemistry / Q000235:genetics
chemical_list: D004247:DNA; D013937:Thymidine Kinase; D003842:Deoxycytidine Kinase; D011505:Protein-Tyrosine Kinases