title: Single-dose concentrations of tinidazole in gingival crevicular fluid, serum, and gingival tissue in adults with periodontitis.
abstract: Previous studies have shown that metronidazole is effective in the treatment of subgingival microflora associated with destructive periodontitis. The aim of this study was to determine whether tinidazole, a close analogue of metronidazole, would reach sufficient concentrations in serum, gingival crevicular fluid, and gingival tissue, to inhibit putative periodontopathic bacteria. Ten adult patients with moderate to advanced periodontitis took a single 2-g dose of tinidazole orally. Samples were assayed by high-performance liquid chromatography. The concentrations of tinidazole in serum and GCF were in a similar range (3.2-46.5 micrograms/mL). Tinidazole was not detected in the GCF in three of the patients. The drug was found in gingival tissue obtained at two h (0.17 +/- 0.14 micrograms/mg) and six h (0.15 +/- 0.18 micrograms/mg) after oral administration. The mean concentration of tinidazole in serum at 24 h (13 +/- 3.0 micrograms/mL) is greater than the minimum inhibitory concentration for anaerobic bacteria as reported by others. The present data suggest that a single 2-g oral dose of tinidazole may lead to the presence of potentially bactericidal levels of tinidazole for up to 24 h in the periodontal pockets of some patients with periodontitis.
authors.affiliation: Periodontics Unit, Westmead Hospital Dental Clinical School, NSW, Australia.
authors.forename: V
authors.initials: V
authors.lastname: Liew
authors.forename: G
authors.initials: G
authors.lastname: Mack
authors.forename: P
authors.initials: P
authors.lastname: Tseng
authors.forename: M
authors.initials: M
authors.lastname: Cvejic
authors.forename: M
authors.initials: M
authors.lastname: Hayden
authors.forename: N
authors.initials: N
authors.lastname: Buchanan
mesh_terms: D000284:Administration, Oral; D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005881:Gingiva / Q000737:chemistry*; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000188:drug therapy* / Q000378:metabolism; D013997:Time Factors; D014011:Tinidazole / Q000008:administration & dosage / Q000032:analysis* / Q000097:blood / Q000627:therapeutic use
chemical_list: D014011:Tinidazole