Protein kinase C(19-31) pseudosubstrate inhibition of insulin action in rat adipocytes.
Treatment of intact adipocytes with the autoregulatory PKC pseudosubstrate PKC(19-31) inhibited insulin-stimulated hexose uptake and lipogenesis, with no effect on basal values. The effect was dose-dependent with respective IC50 values of 30 microM and 600 microM for insulin-stimulated hexose uptake in electroporated and intact adipocytes. These studies indicate that PKC may play a role in the mediation of insulin action in adipocytes.
Standaert
M L
ML
James A. Haley Veterans' Administration Hospital, Tampa, FL 33612.
Sasse
J
J
Cooper
D R
DR
Farese
R V
RV
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D006601:Hexoses / Q000378:metabolism; D007328:Insulin / Q000502:physiology; D007329:Insulin Antagonists / Q000494:pharmacology*; D008055:Lipids / Q000096:biosynthesis; D008969:Molecular Sequence Data; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D051381:Rats; D013379:Substrate Specificity
D006601:Hexoses; D007328:Insulin; D007329:Insulin Antagonists; D008055:Lipids; D011493:Protein Kinase C