Expression of IL-2 receptor p55 and p75 chains by human B lymphocytes: effects of activation and differentiation.
Whilst B cells in human blood can be shown to express interleukin-2 receptor (IL-2R) p55 and p75 chains, using a high-sensitivity immunofluorescence procedure, fresh tonsil B cells did not show detectable levels of expression. Culture of tonsil B cells led to low levels of expression of the p55 chain of the IL-2R, an effect which was dependent on protein synthesis. The level of expression of IL-2R chains could be modulated by culturing in the presence of a number of factors which activate B cells. p55 levels were more readily modulated than p75 levels. IL-4 and combinations of IL-4 with anti-IgM, IL-2 or tumour necrosis factor-beta (TNF-beta) modulated p55 levels, but IL-5 did not. Changes in IL-2R expression were small when compared with other B-cell activation markers such as CD23. When unfractionated tonsil cells were activated with a polyclonal stimulus, the major change was the expression of p55 by T-cell blasts--p75 expression remained low in T and B cells, and p55 expression by B cells remained low.
Zola
H
H
Department of Clinical Immunology, Flinders Medical Centre, Bedford Park, South Australia.
Weedon
H
H
Thompson
G R
GR
Fung
M C
MC
Ingley
E
E
Hapel
A J
AJ
D001402:B-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D002478:Cells, Cultured; D006801:Humans; D015847:Interleukin-4 / Q000276:immunology; D015848:Interleukin-5 / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D014066:Palatine Tonsil / Q000276:immunology; D015375:Receptors, Interleukin-2 / Q000032:analysis*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D015848:Interleukin-5; D015375:Receptors, Interleukin-2; D015847:Interleukin-4