Ro and La antigens and maternal anti-La idiotype on the surface of myocardial fibres in congenital heart block.
Congenital complete heart block (CCHB) is a rare but potentially fatal disease of infants born to mothers with autoimmune disease where maternal autoantibodies to Ro (SS-A) are thought to cross the placenta and damage fetal cardiac tissue. We have adopted a novel approach to demonstrate the localization and specificity of maternal autoantibodies deposited in fetal heart. We raised an anti-idiotype against maternal anti-La antibodies, which reacted strongly with the surface immunoglobulin on the myocardial fibres from a CCHB heart but not a control fetal heart of the same age. Maternal immunoglobulin eluted from the CCHB heart reacted with La (SS-B) by ELISA. Using monoclonal and affinity-purified antibodies to La and affinity-purified anti-Ro antibodies, both antigens were identified on the surface of the fibres of the affected heart. Surface co-expression of immunoglobulin, complement and Class II antigen, consistent with a local immune response, was also found. This is the first definitive demonstration of Ro and La antigens and specific maternal anti-La antibody and idiotype on the surface of myocardial fibres in CCHB. It suggests that induction of Ro and La antigens on the surface of myocardial fibres during fetal development may be critical in the localization of the specific autoantibodies and subsequent evolution of congenital complete heart block.
Horsfall
A C
AC
Kennedy Institute of Rheumatology, London, UK.
Venables
P J
PJ
Taylor
P V
PV
Maini
R N
RN
D000974:Antibodies, Antinuclear; D001324:Autoantigens; D001327:Autoimmune Diseases / Q000150:complications / Q000276:immunology; D005260:Female; D005318:Fetal Heart / Q000276:immunology; D006327:Heart Block / Q000151:congenital* / Q000209:etiology / Q000276:immunology; D006801:Humans; D007130:Immunoglobulin Idiotypes; D007231:Infant, Newborn; D008297:Male; D009206:Myocardium / Q000276:immunology; D011247:Pregnancy; D011248:Pregnancy Complications / Q000276:immunology; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins / Q000276:immunology; D000097585:SS-B Antigen; D000099207:SS-A Antigen
D000974:Antibodies, Antinuclear; D001324:Autoantigens; D007130:Immunoglobulin Idiotypes; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000097585:SS-B Antigen; D000099207:SS-A Antigen; C116006:RO60 protein, human; C035357:SS-B antibodies