Continuous growth of the motor system in the axolotl.
During growth of the axolotl, motor neurons, and muscle fibres are added to the motor system. By double labelling neurons with tritiated thymidine and retrogradely transported HRP, we show that some motor neurons are born at postembryonic stages. Further analysis of motor neurons with the aid of HRP reveals this population of newly born cells relatively frequently in small (5-7 cm long) axolotls, but only rarely in large (7-13 cm long) axolotls. Evidence is presented that suggests that these immature cells are in the process of migrating from close to the ependyma out to the ventral horn. HRP transport also reveals growth cones of advancing axons within spinal nerves in animals up to 6 cm in length. Cell counts by light and electron microscopic methods show that muscle fibres are generated throughout larval life in the iliotibialis, a typical limb muscle. This analysis provides data consistent with the notion that new muscle fibres are added from a localised growth zone situated at the superficial edge of the muscle. These results are discussed in terms of the correlation between continuous growth of the motor system and the ability of the axolotl to functionally repair lesions to the peripheral nervous system.
Holder
N
N
Anatomy and Human Biology, King's College, London, England.
Clarke
J D
JD
Stephens
N
N
Wilson
S W
SW
Orsi
C
C
Bloomer
T
T
Tonge
D A
DA
D000557:Ambystoma / Q000033:anatomy & histology / Q000254:growth & development*; D000818:Animals; D001369:Axons / Q000648:ultrastructure; D006735:Horseradish Peroxidase; D008161:Lumbosacral Region; D008854:Microscopy, Electron; D009046:Motor Neurons / Q000648:ultrastructure*; D024510:Muscle Development; D009132:Muscles / Q000294:innervation; D009186:Myelin Sheath / Q000502:physiology; D009416:Nerve Regeneration / Q000502:physiology*; D013116:Spinal Cord / Q000254:growth & development; D013126:Spinal Nerve Roots / Q000254:growth & development; D013936:Thymidine; D014316:Tritium
D014316:Tritium; D006735:Horseradish Peroxidase; D013936:Thymidine