Haptoglobin typing from fatty tissues after extraction with non-ionic detergents.
Of the seven non-ionic detergents tested, Span 40, Tween 20 and Triton X-45 were suitable for extraction of haptoglobin (Hp) from fatty tissues. The extracted samples were subjected to electrophoresis and subsequently stained with o-tolidine. Enzyme-immunoassay and Western blotting (electrophoretic) techniques were used successfully to determine the Hp phenotypes from minute quantities of tissue samples after extractions with non-ionic detergents.
Roy
R
R
Nebraska State Patrol Crime Laboratory, Lincoln 68502.
D000273:Adipose Tissue / Q000737:chemistry*; D015153:Blotting, Western; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D006242:Haptoglobins / Q000032:analysis / Q000302:isolation & purification*; D006801:Humans; D007124:Immunoenzyme Techniques; D015203:Reproducibility of Results; D012995:Solubility
D003902:Detergents; D006242:Haptoglobins