Repeated intraperitoneal injections of interleukin 1 beta induce glucose intolerance in normal rats.
Previous in vitro findings suggest the involvement of interleukin 1 (IL-1) in the pathogenesis of insulin-dependent diabetes mellitus. The aims of the present study were to investigate the effects of single or repeated ip injections of recombinant IL-1 beta on blood glucose and glucose tolerance in vivo. Normal Wistar Kyoto rats were injected ip with a single injection of 4 micrograms/kg of the mature form of recombinant IL-1 beta (amino acids 117-269) or once daily on 5 consecutive days. Control rats were given vehicle and were fed ad libitum or pair-fed together with the rIL-1 beta treated rats. An ip glucose tolerance test (0.2 g D-glucose/100 g) was performed 2 h after injection of rIL-1 beta. A single injection of rIL-1 beta caused a mild depression in blood glucose and an improved glucose tolerance. Multiple injections of rIL-1 beta induced a diminished weight gain, a 24-28% reduction in food intake, a lasting mild depression of blood glucose (7 days) and a transiently impaired glucose tolerance on day 5. We conclude that systemic IL-1 should be considered an important regulator of glucose homeostasis in vivo.
Wogensen
L
L
Steno Memorial Hospital, Gentofte, Denmark.
Reimers
J
J
Mandrup-Poulsen
T
T
Nerup
J
J
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D001831:Body Temperature / Q000187:drug effects / Q000502:physiology; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000503:physiopathology; D004305:Dose-Response Relationship, Drug; D004435:Eating / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D005951:Glucose Tolerance Test; D006706:Homeostasis / Q000187:drug effects / Q000502:physiology; D007274:Injections, Intraperitoneal; D007375:Interleukin-1 / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology*; D007457:Iodine Radioisotopes; D008297:Male; D051381:Rats; D011921:Rats, Inbred WKY; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology
D001786:Blood Glucose; D007375:Interleukin-1; D007457:Iodine Radioisotopes; D011994:Recombinant Proteins; D005947:Glucose