Haemoglobin phenotypes of the wild European mouflon sheep living on the island of Sardinia.
Haemoglobin (Hb) phenotypes have been studied in 100 wild European mouflons living on the island of Sardinia by means of isoelectric focusing (pH 6.7-7.7 range) of the native tetramers, acid-urea-Triton gel-electrophoresis, and reversed-phase HPLC of globin chains. The result indicates the presence of two beta-globin alleles one of which, corresponding to the beta B, being the most common (f = 0.94). None were carriers of the earlier described Hb A. The new Hb was provisionally named Hb M. Severely anaemic mouflons were able to synthesize Hb C at expense of the Hb B alone, thus suggesting structural and physiological homologies between mouflon beta B and sheep beta A globin genes, and between the newly observed beta M allele and the beta B of the domestic Sardinian sheep.
Naitana
S
S
Institute of Physiology of Domestic Animals, Faculty of Veterinary Medicine, University of Sassari, Italy.
Ledda
S
S
Cocco
E
E
Manca
L
L
Masala
B
B
D000483:Alleles; D000740:Anemia / Q000235:genetics / Q000662:veterinary; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005787:Gene Frequency; D006580:Genetic Carrier Screening; D014644:Genetic Variation / Q000235:genetics; D005914:Globins / Q000235:genetics*; D006454:Hemoglobins / Q000235:genetics*; D007525:Isoelectric Focusing; D007558:Italy; D046911:Macromolecular Substances; D008297:Male; D010641:Phenotype; D011110:Polymorphism, Genetic / Q000235:genetics*; D012756:Sheep / Q000235:genetics*; D012757:Sheep Diseases / Q000235:genetics
D006454:Hemoglobins; D046911:Macromolecular Substances; D005914:Globins