title: Starch bioavailability in the upper gastrointestinal tract of colectomized rats.
abstract: Colectomized rats were used to compare the bioavailability of starch from canned peas, kidney beans, lima beans, corn, cooked rice and AIN-76A purified diet. Postoperative weight gains of colectomized and sham-operated rats were not different. Test meals of approximately 2 and approximately 3.3 g (dry weight) of each food containing chromic oxide (0.6 g/100 g) were administered to 4-6 rats by gastric intubation. Chromium recovery in ileal digesta 10 h after the meal ranged from 94 to 102%. Significant starch (11-15%) from peas, lima beans or kidney beans was recovered in the ileal digesta; 0.2-0.4% of starch from rice, corn or AIN-76A was in the ileal digesta. Pretreatment of kidney or lima beans with a heat-stable endo-alpha-amylase decreased the starch recovered in the ileal digesta to 2.7-4.3% of that fed; pretreatment with the amylase and pepsin had no further effect. Oligosaccharide extraction, the size of the test meal and the amount of starch did not affect starch bioavailability.
authors.affiliation: Department of Nutritional Sciences, University of Wisconsin-Madison 53706.
authors.forename: L A
authors.initials: LA
authors.lastname: Hildebrandt
authors.forename: J A
authors.initials: JA
authors.lastname: Marlett
mesh_terms: D000818:Animals; D001682:Biological Availability; D003082:Colectomy; D004040:Dietary Carbohydrates / Q000493:pharmacokinetics*; D004064:Digestive System / Q000378:metabolism*; D007887:Fabaceae; D008297:Male; D012275:Oryza; D010946:Plants, Medicinal; D051381:Rats; D013213:Starch / Q000493:pharmacokinetics*; D015430:Weight Gain; D003313:Zea mays
chemical_list: D004040:Dietary Carbohydrates; D013213:Starch