Transformation of the filamentous fungus Gibberella fujikuroi using the Aspergillus niger niaD gene encoding nitrate reductase.
A transformation system for Gibberella fujikuroi based on the Aspergillus niger nitrate reductase gene (niaD) was developed. A strain (designated SG140) carrying a non-reverting niaD mutation (niaD11) was generated by screening mutagenised cells for non-growth on nitrate as sole nitrogen source. Transformation frequencies of 1-2 transformants per microgram DNA were observed when strain SG140 was transformed to nitrate utilisation. Southern blot analyses of niaD+ transformants showed that the vector DNA sequences were integrated into the chromosomal DNA. The results demonstrate that the A. niger niaD gene is expressed in G. fujikuroi.
Sànchez-Fernàndez
R
R
Departamento de Genetica y Biotecnia, Universidad de Sevilla, Spain.
Unkles
S E
SE
Campbell
E I
EI
Macro
J A
JA
Cerdà-Olmedo
E
E
Kinghorn
J R
JR
D001234:Aspergillus niger / Q000201:enzymology / Q000235:genetics*; D015139:Blotting, Southern; D005874:Gibberella / Q000235:genetics*; D016296:Mutagenesis; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D014170:Transformation, Genetic
D009565:Nitrate Reductases; D050901:Nitrate Reductase