Singlet oxygen induced single-strand breaks in plasmid pBR322 DNA: the enhancing effect of thiols.
The biologically occurring thiols, glutathione, cysteamine and cysteine, significantly enhance the single-strand breaks in plasmid pBR322 DNA induced by singlet molecular oxygen (1O2) generated by the thermodissociation of the endoperoxide of 3,3'-(1,4-naphthylidene)dipropionate. The enhancing effect was also observed with chemically related sulfhydryl compounds but not by disulfides. In contrast, dihydrolipoate and its disulfide lipoate protected the plasmid DNA. Metal chelators as well as superoxide dismutase or catalase had no effect, whereas mannitol or sodium azide, decreased the thiol-1O2-induced strand breaks. It is concluded that the observed effects are mediated by reactive oxidation products arising from the 1O2-oxidation of thiols.
Devasagayam
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Institut für Physiologische Chemie I, Universität Düsseldorf, F.R.G.
Di Mascio
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Kaiser
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Sies
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D002614:Chelating Agents; D003543:Cysteamine / Q000494:pharmacology; D003545:Cysteine / Q000494:pharmacology; D004249:DNA Damage; D004357:Drug Synergism; D004587:Electrophoresis, Agar Gel; D005609:Free Radicals; D005978:Glutathione / Q000494:pharmacology; D010100:Oxygen; D010957:Plasmids; D013438:Sulfhydryl Compounds / Q000494:pharmacology*
D002614:Chelating Agents; D005609:Free Radicals; D013438:Sulfhydryl Compounds; D003543:Cysteamine; D005978:Glutathione; D003545:Cysteine; D010100:Oxygen