Nuclear and cytoplasmic lectin binding sites in promastigotes of Leishmania.
We demonstrated the presence of intracellular lectin binding sites in promastigotes of Leishmania mexicana amazonensis. Direct and indirect lectin-gold techniques were used on Lowicryl K4M-embedded cells. The nuclear compartment was labeled by most lectins. Nucleoli were mainly labeled by WFH (Wistaria floribunda hemagglutinin) and LFA (Limax flavus agglutinin) specific for D-galactose/N-acetyl-D-galactosamine (D-Gal/D-GalNAc) and sialic acid, respectively. Sections treated with the fetuin-gold complex without previous lectin incubation also exhibited labeled nucleoli, although less intensely, suggesting the presence not only of sialic acid but also of a sialic acid-specific endogenous carbohydrate binding molecule in Leishmania nuclei. Surprisingly, the Golgi complex was never labeled, whereas the endoplasmic reticulum was frequently labeled, especially by RCA (Ricinus communis agglutinin; D-GalNAc/D-Gal) and WGA (wheat germ agglutinin; D-GlcNAc). The kinetoplast, a DNA-containing structure located within the mitochondrion, was generally labeled towards its extremities, where previous studies have shown the presence of ribonucleoproteins. Some possible biological roles for these intracellular glycoconjugates are discussed.
Vannier-Santos
M A
MA
Laboratório de Ultraestrutura Celular e Microscopia Eletrônica, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brasil.
Saraiva
E M
EM
de Souza
W
W
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D003593:Cytoplasm / Q000378:metabolism* / Q000648:ultrastructure; D006001:Glycoconjugates / Q000378:metabolism; D007150:Immunohistochemistry; D007425:Intracellular Membranes / Q000378:metabolism / Q000648:ultrastructure; D037102:Lectins / Q000378:metabolism*; D007894:Leishmania mexicana / Q000378:metabolism* / Q000648:ultrastructure; D009697:Nucleolus Organizer Region / Q000378:metabolism / Q000648:ultrastructure
D006001:Glycoconjugates; D037102:Lectins