Six chains of the human T cell antigen receptor.CD3 complex are necessary and sufficient for processing the receptor heterodimer to the cell surface.
The T cell antigen receptor (TCR) plays a key role in the process of antigen recognition. It is a complex of at least seven peptide chains (alpha beta gamma delta epsilon zeta-zeta). It is found on the surface of mature T cells and functions in antigen binding in the presence of the major histocompatibility complex. It has been known for some time that physical associations between the CD3 proteins and the TCR chains are essential for efficient transport of either component to the surface of T cells. For example, T cells that lack either the alpha, beta, or delta chains synthesize partial complexes that are eventually degraded. cDNAs encoding the six chains of receptor have become available recently. We have used transfection techniques to generate a panel of Chinese hamster ovary cells that contain partial receptor complexes of known composition and also cells that express all six subunits of the TCR.CD3 complex. Cells in this panel were analyzed for the ability to form alpha-beta heterodimers and also an ability to transport the synthesized chains to the plasma membrane. These studies have allowed us to define the minimum requirements for TCR.CD3 expression on the cell surface.
Carson
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GR
Protein Expression Group, T Cell Sciences, Inc., Cambridge, Massachusetts 02139.
Kuestner
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Ahmed
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A
Pettey
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CL
Concino
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MF
D000818:Animals; D015152:Blotting, Northern; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D010957:Plasmids; D011233:Precipitin Tests; D012333:RNA, Messenger / Q000032:analysis; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection
D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell; D004247:DNA