title: Subsite specificity of the proteinase from myeloblastosis associated virus.
abstract: The subsite requirements of the aspartic proteinase from the myeloblastosis-associated virus (MAV) for the cleavage of peptide substrates were studied with a series of synthetic peptides of general structure Ala-Thr-P4-P3-P2-P1*Nph-Val-Arg-Lys-Ala. The residues in positions P4, P3, P2 and P1 were varied and the kinetic parameters for the cleavage of substrates in 2.0 M NaCl were spectrophotometrically determined at pH 6.0 and 37 degrees C. The acceptance of amino acid residues in particular subsites is similar to that observed with the human immunodeficiency virus type 1 (HIV-1) proteinase in our earlier studies on the same substrate series: hydrophobic or aromatic residues are preferable in P1 position, a broad variety of residues are acceptable in P3 whereas the residues occupying P2 plays the decisive role in the substrate cleavage as evidenced by its dramatic influence on both kcat and Km values. The most remarkable difference between the two enzymes was found in P3 and P4 subsites. In P3, the introduction of negatively charged glutamate increases the substrate binding by the MAV proteinase 12-fold and decreases binding by the HIV-1 proteinase. In P4, Pro in this series is a favourable residue for the MAV proteinase and is strongly inacceptable for HIV-1 the proteinase. The pH profile of the cleavage was studied with a chromogenic substrate and differences between HIV-1 and MAV proteinases are discussed.
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Science, Prague.
authors.forename: J
authors.initials: J
authors.lastname: Konvalinka
authors.forename: I
authors.initials: I
authors.lastname: Blaha
authors.forename: R
authors.initials: R
authors.lastname: Skrabana
authors.forename: J
authors.initials: J
authors.lastname: Sedlacek
authors.forename: I
authors.initials: I
authors.lastname: Pichova
authors.forename: F
authors.initials: F
authors.lastname: Kapralek
authors.forename: V
authors.initials: V
authors.lastname: Kostka
authors.forename: P
authors.initials: P
authors.lastname: Strop
mesh_terms: D000595:Amino Acid Sequence; D016282:Aspartic Acid Endopeptidases / Q000378:metabolism*; D009189:Avian Myeloblastosis Virus / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008969:Molecular Sequence Data; D013057:Spectrum Analysis; D013379:Substrate Specificity
chemical_list: D016282:Aspartic Acid Endopeptidases; C036946:protease p15