title: Prerequisites for successful human sperm cryobanking: sperm quality and prefreezing holding time.
abstract: Frozen-thawed donor semen was used in artificial inseminations and in vitro fertilization programs. Semen accepted for donation was characterized (mean +/- SE) by sperm concentration of 150 +/- 18.6 x 10(6)/mL, normal morphology of 57% +/- 1.4%, good progressive motility at 1 hour of 57% +/- 1.0%, and post-thaw motility of 45% +/- 1.0%. Delay of the freezing process for greater than 1 hour after semen delivery caused a deleterious effect to the freezability of sperm. The average monthly fecundability for the 1st 6 months after inseminations was 13.6%. This value decreased dramatically to 2.6% from the 7th month onward. In 74 IVF/embryo transfer (ET) cycles, the fertilization rate was 55.3% +/- 3.8%, pregnancy rate (PR) per ET was 39.6%, and the PR per woman was 42.8%.
authors.affiliation: Institute for the Study of Fertility, Serlin Maternity Hospital, Sackler School of Medicine, Tel Aviv University, Israel.
authors.forename: H
authors.initials: H
authors.lastname: Yavetz
authors.forename: L
authors.initials: L
authors.lastname: Yogev
authors.forename: Z
authors.initials: Z
authors.lastname: Homonnai
authors.forename: G
authors.initials: G
authors.lastname: Paz
mesh_terms: D015925:Cryopreservation; D005307:Fertilization in Vitro; D006801:Humans; D007315:Insemination, Artificial; D008297:Male; D008954:Models, Biological; D013074:Sperm Banks; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology; D013997:Time Factors; D014019:Tissue Donors