Cinematographic analysis of vascular smooth muscle cell interactions with extracellular matrix.
The interactions of vascular smooth muscle cells with growth modulators and extracellular matrix molecules may play a role in the proliferation and migration of these cells after vascular injury and during the development of atherosclerosis. Time-lapse cinematographic techniques have been used to study cell division and migration of bovine carotid artery smooth muscle cells in response to matrix molecules consisting of solubilized basement membrane (Matrigel) and type I collagen. When cells were grown adjacent to Matrigel, both migration and cell proliferation were increased and interdivision time was shortened. Cells grown in Matrigel or in type I collagen had markedly reduced migration rates but interdivision time was not altered. Further, diffusible components of the Matrigel were found to stimulate proliferation of the smooth muscle cells.
Absher
M
M
Department of Medicine, University of Vermont, Burlington 05405.
Baldor
L
L
D000818:Animals; D001485:Basement Membrane / Q000502:physiology; D001672:Biocompatible Materials / Q000494:pharmacology; D002339:Carotid Arteries / Q000166:cytology / Q000502:physiology; D002417:Cattle; D002455:Cell Division; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology; D004338:Drug Combinations; D005109:Extracellular Matrix / Q000502:physiology* / Q000648:ultrastructure; D007700:Kinetics; D007797:Laminin / Q000494:pharmacology; D009040:Motion Pictures; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000502:physiology*; D011509:Proteoglycans / Q000494:pharmacology; D013997:Time Factors
D001672:Biocompatible Materials; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen