Enhancement of glutathione levels in mouse peritoneal macrophages by sodium arsenite, cadmium chloride and glucose/glucose oxidase.
Glutathione content of mouse peritoneal macrophages markedly increased when they were exposed to insulting agents like sodium arsenite, cadmium chloride, and glucose/glucose oxidase which generates hydrogen peroxide. This increase was attributed to the induction of the cystine transport activity by these agents. The transport activity for other amino acids was not induced, but rather diminished by these agents. Heat shock treatment did not induce the cystine transport activity, nor did it augment glutathione. Since glutathione protects cells against the cytotoxic effects of these agents, the induction of the cystine transport activity constitutes a protective mechanism related to the stress caused by the agents. The protein component(s) for cystine transport may fall into the category of the stress protein.
Bannai
S
S
Department of Biochemistry, Tsukuba University Medical School, Ibaraki, Japan.
Sato
H
H
Ishii
T
T
Taketani
S
S
D000818:Animals; D001151:Arsenic / Q000494:pharmacology*; D018053:Arsenites; D001692:Biological Transport; D002104:Cadmium / Q000494:pharmacology*; D019256:Cadmium Chloride; D002478:Cells, Cultured; D003553:Cystine / Q000378:metabolism; D005260:Female; D005947:Glucose / Q000494:pharmacology*; D005949:Glucose Oxidase / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D010529:Peritoneal Cavity / Q000166:cytology*
D018053:Arsenites; D002104:Cadmium; D003553:Cystine; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005978:Glutathione; D005947:Glucose; D019256:Cadmium Chloride; C015001:arsenite; D001151:Arsenic