title: Demonstration by western blotting of antiheart antibodies before and after cardiac transplantation.
abstract: Western blotting has been used to detect antiheart antibodies in two groups of patients: two who required retransplantation for hyperacute rejection, and 22 consecutive patients, whose serum was tested at monthly intervals for three months following transplantation. Pretransplant and posttransplant serum samples were tested for IgM and IgG reactivity against the patients own heart and donor heart. In all patients the pretransplant lymphocytic crossmatch had been negative. In the two patients requiring retransplantation, both had multiple bands of strong IgM and IgG against their own heart prior to transplantation as well as antibodies against the donor heart. The study of 22 consecutive patients revealed that (1) the presence of strong antibody prior to transplantation is associated with unusually severe or frequent rejection episodes, (2) 20/22 patients made antiheart antibody following transplantation, but in 12 patients it was IgM only, and (3) most of the antiheart antibodies made posttransplant were not specific for the donor heart. Comparison of Western blotting with immunofluorescent detection of antibodies on frozen sections revealed that the Western blotting procedure is more sensitive and results are easier to interpret.
authors.affiliation: Department of Cardiothoracic Surgery, National Heart and Lung Institute, London, United Kingdom.
authors.forename: M J
authors.initials: MJ
authors.lastname: Dunn
authors.forename: M L
authors.initials: ML
authors.lastname: Rose
authors.forename: N
authors.initials: N
authors.lastname: Latif
authors.forename: S
authors.initials: S
authors.lastname: Bradd
authors.forename: C
authors.initials: C
authors.lastname: Lovegrove
authors.forename: C
authors.initials: C
authors.lastname: Seymour
authors.forename: A
authors.initials: A
authors.lastname: Pomerance
authors.forename: M H
authors.initials: MH
authors.lastname: Yacoub
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000906:Antibodies / Q000032:analysis*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D005455:Fluorescent Antibody Technique; D006084:Graft Rejection; D016027:Heart Transplantation / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000276:immunology* / Q000473:pathology; D012967:Sodium Dodecyl Sulfate; D013997:Time Factors
chemical_list: D000906:Antibodies; D012967:Sodium Dodecyl Sulfate