Evaluation of AN-Ident.
AN-Ident (Analytab Products, Inc., Plainview, N.Y.) is a ready-to-use system for anaerobe identification. It is based on the detection of constitutive preformed enzymes, is growth independent, and requires only 4 h of aerobic incubation. This micromethod was evaluated for its ability to identify anaerobic bacteria by using a conventional methodology as a reference. Of 265 clinical isolates, AN-Ident accurately identified 241 (91%) of the isolates to the species level and 259 (98%) of the isolates to the genus level, with limited supplemental testing needed (5%). The AN-Ident system performed well for the most common pathogens but less satisfactorily for infrequently isolated and/or asaccharolytic species; expansion and updating of the data base would be helpful. Although some color reactions were difficult to interpret, the commercial kit was easy to use.
Quentin
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Laboratoire de Bactériologie, Hôpital Pellegrin, Bordeaux, France.
Desailly-Chanson
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Bebear
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D001421:Bacteria, Anaerobic / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis*; D001431:Bacteriological Techniques; D001439:Bacteroides / Q000302:isolation & purification; D003013:Clostridium / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D006801:Humans