title: Slower growth of tumours in sympathetically denervated murine skin.
abstract: In order to examine tumour growth in sympathetically denervated murine skin, two breast cancer tumour lines were employed, i.e. M3 tumours, of a relatively high local growth and low metastatic capacity, and MM3-LN tumours, that grew locally at a slower rate but disseminated early to the lung. Mice subjected to unilateral superior cervical ganglionectomy or sham-operation 2 weeks earlier were used. M3 or MM3-LN tumours were implanted in the ipsilateral ear to the surgical procedure. Tumour size was assessed every 2-6 days, starting from the 7th day after tumour implantation. Growth of M3 and MM3-LN tumours was significantly slowed by a previous sympathetic denervation of the skin territory. There were no significant differences in the number or size of pulmonary metastases at autopsy between mice subjected to ganglionectomy or to sham-operation. Ganglionectomy increased significantly ipsilateral submaxillary lymph node ornithine decarboxylase activity by 62% and decreased noradrenaline content to 8% of the innervated contralateral lymph node. The present results indicate a local inhibitory modulation of tumour growth by the sympathetic nervous system.
authors.affiliation: Departamento de Fisiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
authors.forename: H E
authors.initials: HE
authors.lastname: Romeo
authors.forename: L L
authors.initials: LL
authors.lastname: Colombo
authors.forename: A I
authors.initials: AI
authors.lastname: Esquifino
authors.forename: R E
authors.initials: RE
authors.lastname: Rosenstein
authors.forename: H E
authors.initials: HE
authors.lastname: Chuluyan
authors.forename: D P
authors.initials: DP
authors.lastname: Cardinali
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology*; D000818:Animals; D005728:Ganglia, Sympathetic / Q000502:physiology*; D015171:Ganglionectomy; D008175:Lung Neoplasms / Q000473:pathology / Q000556:secondary*; D008198:Lymph Nodes / Q000737:chemistry / Q000201:enzymology; D008325:Mammary Neoplasms, Experimental / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D009638:Norepinephrine / Q000032:analysis; D009955:Ornithine Decarboxylase / Q000378:metabolism; D012867:Skin / Q000294:innervation* / Q000473:pathology; D014407:Tumor Cells, Cultured
chemical_list: D009955:Ornithine Decarboxylase; D009638:Norepinephrine