Mutations in yeast U5 snRNA alter the specificity of 5' splice-site cleavage.
Recognition of 5' splice sites in pre-mRNA splicing is achieved in part by base pairing with U1 snRNA. We have used interactive suppression in the yeast Saccharomyces cerevisiae to look for other factors involved in 5' splice-site recognition. This approach identified an extragenic suppressor that activates a cryptic 5' splice site. The suppressor is a gene for U5 snRNA (snR7) with a single base mutation in a strictly conserved 9 base sequence. This suggests that U5 snRNA can play a part in determining the position of 5' splice-site cleavage. Consistent with this, we have been able to isolate other mutations in the 9 base element in U5 snRNA that specifically activate a second cryptic 5' splice site nearby.
Newman
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A
MRC Laboratory of Molecular Biology, Cambridge, England.
Norman
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C
D001482:Base Composition; D001483:Base Sequence; D005800:Genes, Fungal; D016153:Genes, Suppressor; D007438:Introns; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D012322:RNA Precursors / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012326:RNA Splicing / Q000235:genetics*; D012331:RNA, Fungal / Q000378:metabolism; D012342:RNA, Small Nuclear / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013379:Substrate Specificity
D012322:RNA Precursors; D012331:RNA, Fungal; D012342:RNA, Small Nuclear