Regional metabolic rate of exogenous glucose in the isoprenaline and dobutamine stimulated canine myocardium as estimated by the 2-deoxy-D[1-14C]glucose method.
The effect of beta-adrenoceptor stimulation by isoprenaline and dobutamine on the transmural distribution pattern of regional myocardial metabolic rate of exogenous glucose (RMMRGlc) was studied in the anesthetized closed chest dog using the 2-deoxy-D[1-14C]glucose method. In a previous series a lumped constant (LC) value of 0.93 +/- 0.47 (1 SD) was measured for [14C]2-deoxyglucose in the canine myocardium. In the control group (N = 12) RMMRGlc was significantly higher in the subendocardial layer of the left ventricular free wall than in both the middle and subepicardial layer, where it was quite evenly distributed (P less than or equal to 0.05). With i.v. dobutamine (N = 8) RMMRGlc was significantly lower in the midportion of left ventricular free wall than in the subepicardial layer (P less than or equal to 0.05), but it was not different from the inner wall section. Significant differences between the subepicardial and subendocardial portions of the left ventricular free wall could not be found, either. In the isoprenaline group (N = 9) no transmural gradients of RMMRGlc were observed in the left ventricular myocardium. In all groups, both the interventricular septum and the right ventricular free wall exhibited homogeneous distribution patterns of RMMRGlc. It is concluded that transmural distribution patterns of exogenous glucose utilization probably reflect corresponding gradients in energy demands of the left ventricular wall. Redistribution of RMMRGlc in the isoprenaline and dobutamine groups may result from altered working conditions, a change in local inotropic state of the left ventricular myocardium, or from regional differences in the proportions of substrate utilization, and from regional differences in adrenoceptor density.
Rubart
M
M
Department of Internal Medicine I, RWTH Aachen, F.R.G.
Breull
W
W
Hahn
N
N
D000818:Animals; D002250:Carbon Radioisotopes; D003847:Deoxyglucose; D004280:Dobutamine / Q000494:pharmacology*; D004285:Dogs; D005260:Female; D005947:Glucose / Q000378:metabolism*; D006321:Heart / Q000187:drug effects; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D013268:Stimulation, Chemical
D002250:Carbon Radioisotopes; D004280:Dobutamine; D003847:Deoxyglucose; D005947:Glucose; D007545:Isoproterenol