title: Recombinant human basic fibroblast growth factor (rhbFGF) induces secretion of nerve growth factor (NGF) in cultured rat astroglial cells.
abstract: Recombinant human basic fibroblast growth factor (rhbFGF) was found to induce secretion of nerve growth factor (NGF) in cultured astrocytes from rat brain. The induction was concentration dependent; a maximum increase of 2-fold was observed at concentrations of 3-10 ng/ml or rhbFGF. When rhbFGF was added to the astrocytes, the induction occurred within 8 h and leveled off after 12 h. Antiserum against rhbFGF completely blocked the induction. Maximum induction occurred under serum-free culture conditions and in cultures with a high cell density. These results suggest that rhbFGF may exert a neurotrophic effect on the central nervous system through the induction of NGF secretion by astrocytes.
authors.affiliation: Biology Research Laboratories, Takeda Chemical Ind., Ltd., Osaka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Fukumoto
authors.forename: M
authors.initials: M
authors.lastname: Kakihana
authors.forename: M
authors.initials: M
authors.lastname: Suno
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000187:drug effects / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016222:Fibroblast Growth Factor 2 / Q000276:immunology / Q000494:pharmacology*; D007106:Immune Sera; D007700:Kinetics; D009414:Nerve Growth Factors / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000276:immunology / Q000494:pharmacology
chemical_list: D007106:Immune Sera; D009414:Nerve Growth Factors; D011994:Recombinant Proteins; D016222:Fibroblast Growth Factor 2