c-myc mRNA in cytoskeletal-bound polysomes in fibroblasts.
3T3 fibroblasts were treated sequentially with 25 mM-KCl/0.05% Nonidet P40, 130 mM-KCl/0.05% Nonidet P40 and finally with 1% Nonidet P40/1% deoxycholate in order to release free, cytoskeletal-bound and membrane-bound polysomes respectively. The membrane-bound fraction was enriched in the mRNA for the membrane protein beta 2-microglobulin, whereas the cytoskeletal-bound polysomes were enriched in c-myc mRNA. Actin mRNA was present in both free and cytoskeletal-bound polysomes. The results suggest that cytoskeletal-bound polysomes are involved in the translation of specific mRNA species.
Hesketh
J E
JE
Rowett Research Institute, Bucksburn, Aberdeen, Scotland, U.K.
Campbell
G P
GP
Whitelaw
P F
PF
D000199:Actins / Q000235:genetics; D000818:Animals; D015152:Blotting, Northern; D002458:Cell Fractionation; D002460:Cell Line; D003599:Cytoskeleton / Q000378:metabolism* / Q000648:ultrastructure; D005347:Fibroblasts / Q000378:metabolism; D016259:Genes, myc; D051379:Mice; D009693:Nucleic Acid Hybridization; D011132:Polyribosomes / Q000378:metabolism* / Q000648:ultrastructure; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification*; D015183:Restriction Mapping; D001613:beta 2-Microglobulin / Q000235:genetics
D000199:Actins; D012333:RNA, Messenger; D001613:beta 2-Microglobulin