Isolation and partial characterization of the structures of fibroblast activating factor-related proteins from U937 cells.
Human cultured monocyte-like tumour cells of the U937 histiocyte derived line were stimulated with phorbol myristate acetate, and generated and released an 18,000 MW polypeptide fibroblast-activating factor (FAF). Based on recognition by an antiserum to a synthetic peptide representing the 17 amino-terminal amino acids of FAF, two proteins of 32,000 and 35,000 MW were identified in extracts of U937 cells. Purification of the intracellular FAF-related proteins to homogeneity allowed the generation and amino acid sequencing of nine tryptic fragments of 4-11 amino acids. Neither of the intracellular FAF-related proteins exhibited the fibroblast proliferation-stimulating activity of FAF, suggesting that they are biosynthetic precursors analogous to the inactive propeptides of interleukin-1 beta and tumour necrosis factor-alpha.
Demeter
J
J
Howard Hughes Medical Institute, University of California, San Francisco 94143-0724.
Medzihradszky
D
D
Kha
H
H
Goetzl
E J
EJ
Turck
C W
CW
D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D015153:Blotting, Western; D002460:Cell Line; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D010450:Endopeptidases; D018093:Gelatinases; D006133:Growth Substances / Q000302:isolation & purification*; D006801:Humans; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009000:Monocytes / Q000276:immunology*; D012697:Serine Endopeptidases; D000099167:Fibroblast Activation Protein Alpha
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D010450:Endopeptidases; D018093:Gelatinases; D006133:Growth Substances; D008565:Membrane Proteins; D012697:Serine Endopeptidases; D000099167:Fibroblast Activation Protein Alpha