Odorant-sensitive adenylate cyclase: rapid, potent activation and desensitization in primary olfactory neuronal cultures.
Using primary olfactory neuronal cultures, we have demonstrated rapid, potent increases in cAMP levels and adenylate cyclase [AC; ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] activity in response to odorants. Isobutyl-methoxypyrazine is active at 1 nM. Odorant enhancement is dependent on Ca2+ concentration with maximal effects at 10-100 microM. Biphasic temporal and concentration-related effects occur with all odorants. All odorants examined elicit desensitization with AC responses abolished when odorants are reapplied immediately after removal. When reapplied 1 min after removal, odorants elicit an AC response greater than on first exposure, implying a cellular "memory" for odorants.
Ronnett
G V
GV
Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Parfitt
D J
DJ
Hester
L D
LD
Snyder
S H
SH
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D000081005:Cyclohexane Monoterpenes; D003511:Cyclohexanols / Q000494:pharmacology; D004789:Enzyme Activation; D045782:Hemiterpenes; D007700:Kinetics; D039821:Monoterpenes; D009474:Neurons / Q000201:enzymology*; D009570:Nitriles / Q000494:pharmacology; D009812:Odorants; D009830:Olfactory Bulb / Q000201:enzymology*; D010421:Pentanoic Acids / Q000494:pharmacology; D011719:Pyrazines / Q000494:pharmacology; D051381:Rats; D013729:Terpenes / Q000494:pharmacology
D000081005:Cyclohexane Monoterpenes; D003511:Cyclohexanols; D045782:Hemiterpenes; D039821:Monoterpenes; D009570:Nitriles; D010421:Pentanoic Acids; D011719:Pyrazines; D013729:Terpenes; C008216:isovaleric acid; C067963:coniferan; C006923:carvone; C054764:citralva; D000262:Adenylyl Cyclases; C034376:2-isobutyl-3-methoxypyrazine