title: Serodiagnostic antibody responses to Psoroptes sp. infestations in bighorn sheep.
abstract: The antibody responses of bighorn sheep (Ovis canadensis) infected with Psoroptes sp. mites were investigated by enzyme linked immunosorbent assay on western blots of P. cuniculi antigens. Serum from 20 Psoroptes sp.-infested bighorn sheep (O. canadensis mexicana, O. canadensis nelsoni, O. canadensis canadensis) from New Mexico, Nevada, California, and Idaho reacted strongly with mite antigens ranging from 12 to 34 kd. Serum from 35 Psoroptes sp.-free bighorn sheep of unknown tick infestation status and from three Psoroptes sp.-free bighorn sheep infested with Dermacentor hunteri ticks did not react with these antigens. Psoroptes sp.-specific antibody responses were present throughout a 16 mo period in one infected bighorn sheep, but were not detectable 8 mo following successful treatment. These results demonstrate that specific serodiagnosis of Psoroptes sp. infestation is feasible in bighorn sheep and suggest that antibody responses are indicative of current or recent infestation.
authors.affiliation: Department of Veterinary Microbiology and Immunology, University of California, Davis 95616.
authors.forename: W M
authors.initials: WM
authors.lastname: Boyce
authors.forename: D A
authors.initials: DA
authors.lastname: Jessup
authors.forename: R K
authors.initials: RK
authors.lastname: Clark
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood; D015153:Blotting, Western / Q000662:veterinary; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D008924:Mite Infestations / Q000175:diagnosis / Q000662:veterinary*; D008925:Mites / Q000276:immunology*; D012698:Serologic Tests; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000469:parasitology*
chemical_list: D000906:Antibodies