title: Laser-Doppler measurement of tracheal mucosal blood flow: comparison with tracheal arterial flow.
abstract: Blood flow in the tracheal mucosa (Qm) has been measured by laser-Doppler flowmetry in anesthetized sheep and dogs. The values have been compared with tracheal arterial inflow (Qtr) by use of an electromagnetic flow probe and with tracheal arterial perfusion pressure (Ptr) produced by mechanical perfusion. Changes in blood flow were caused by injections of methacholine, phenylephrine, and histamine into the perfusion circuit. These interventions produced a range of measurements for each animal. Correlations of Qm against Qtr were significant in two of five animals (R = 0.03-0.93); correlations of Qm against Ptr were significant in two of four animals (R = 0.56-0.96). Percent changes in Qtr were generally much larger than those of Qm, and there was considerable variability between Qm and either Qtr or Ptr. Qm reflected the same vascular changes as Ptr or Qtr in 28 interventions and showed an opposing change in 4 cases. In 11 interventions, changes measured by Ptr or Qtr were not reflected by any changes in Qm. Thus qualitative changes in tracheal perfusion measured with these methods were usually the same; quantitatively the three methods showed great differences. These differences may reflect different regulatory mechanisms in various components of the tracheal vasculature or different technical aspects of the methods used.
authors.affiliation: Department of Physiology, St. George's Hospital Medical School, London, United Kingdom.
authors.forename: D R
authors.initials: DR
authors.lastname: Corfield
authors.forename: M E
authors.initials: ME
authors.lastname: Deffebach
authors.forename: I
authors.initials: I
authors.lastname: Erjefält
authors.forename: R O
authors.initials: RO
authors.lastname: Salonen
authors.forename: S E
authors.initials: SE
authors.lastname: Webber
authors.forename: J G
authors.initials: JG
authors.lastname: Widdicombe
mesh_terms: D000818:Animals; D001158:Arteries; D004285:Dogs; D005260:Female; D007834:Lasers; D008297:Male; D016210:Methacholine Chloride / Q000494:pharmacology; D009092:Mucous Membrane / Q000098:blood supply / Q000187:drug effects; D010656:Phenylephrine / Q000494:pharmacology; D012039:Regional Blood Flow / Q000187:drug effects; D012756:Sheep; D014132:Trachea / Q000098:blood supply* / Q000187:drug effects
chemical_list: D016210:Methacholine Chloride; D010656:Phenylephrine