title: Evaluation of a 'dipstick' immunoassay to detect cysticercosis in experimentally infected cattle.
abstract: A 'dipstick' immunoassay for bovine cysticercosis, using an antigen isolated from Taenia hydatigena cyst fluid, was evaluated in cattle experimentally infected with Taenia saginata. The assay correctly identified six out of seven infected cattle, including an animal in which only 12 living cysticerci were found. Cattle became seropositive as early as 3 weeks post-infection. A false-negative reaction was found for one very lightly infected animal, from which only four living cysticerci were recovered at necropsy. The assay was also used to detect circulating antibodies in experimentally infected cattle before and after therapeutic treatment with anthelminthics. The results suggest that praziquantel-treated animals gradually revert to being seronegative after the cysticerci are killed.
authors.affiliation: U.S. Department of Agriculture, Food Safety and Inspection Service, Beltsville, MD 20705.
authors.forename: E G
authors.initials: EG
authors.lastname: Hayunga
authors.forename: M M
authors.initials: MM
authors.lastname: Wong
authors.forename: M P
authors.initials: MP
authors.lastname: Sumner
authors.forename: R S
authors.initials: RS
authors.lastname: Isenstein
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D003551:Cysticercosis / Q000175:diagnosis / Q000662:veterinary*; D005069:Evaluation Studies as Topic; D005188:False Negative Reactions; D007118:Immunoassay / Q000379:methods*; D011237:Predictive Value of Tests; D013621:Taenia / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth