title: Superiority of the use of a pre-incubated complex of primary and second antibody in the radioimmunoassay of thyroid hormones.
abstract: We report on the superiority of a radioimmunoassay (RIA) system wherein the second antibody (Ab2) is incorporated as a pre-incubated complex with the primary antibody (Ab1) for the assay of haptens like triiodothyronine (T3) and thyroxine (T4). Separation of the antibody bound and free antigen (Ag) was accomplished by 8% polyethylene glycol (PEG) (final concentration) following a single incubation of less than 1 h. The other advantages of this system are a 15-fold reduction in the quantity of Ab2 (without any need for increasing the concentration of PEG) and the consequent savings in cost.
authors.affiliation: Board of Radiation and Isotope Technology, BARC Vashi Complex, New Bombay, India.
authors.forename: A
authors.initials: A
authors.lastname: Rathinaswamy
authors.forename: U H
authors.initials: UH
authors.lastname: Nagvekar
authors.forename: J
authors.initials: J
authors.lastname: Chatur
authors.forename: V
authors.initials: V
authors.lastname: Nirmala
authors.forename: K
authors.initials: K
authors.lastname: Rani
authors.forename: N
authors.initials: N
authors.lastname: Ramamoorthy
authors.forename: S A
authors.initials: SA
authors.lastname: Balakrishnan
authors.forename: C N
authors.initials: CN
authors.lastname: Desai
mesh_terms: D003365:Costs and Cost Analysis; D006801:Humans; D011863:Radioimmunoassay / Q000379:methods*; D011933:Reagent Kits, Diagnostic / Q000191:economics; D013963:Thyroid Hormones / Q000032:analysis*
chemical_list: D011933:Reagent Kits, Diagnostic; D013963:Thyroid Hormones