The relative role of neutrophils and platelets in the local accumulation of circulating lymphocytes at sites of ionophore A23187 inoculation.
The early cellular infiltrate at inflammatory sites consists predominantly of neutrophils and cells of the monocyte/macrophage lineage. The mechanism by which circulating, unsensitized lymphocytes accumulate at sites of inflammation is unknown. The pattern of accumulation of 111indium-labeled circulating thymocytes in response to local injections of the ionophore A23187 was studied and compared with the pattern of (125)iodinated albumin accumulation as a measure of vascular permeability. The kinetics of thymocyte accumulation differed from those of vascular permeability. Sublethal total-body irradiation (750 rads) markedly decreased thymocyte accumulation but had little effect on vascular permeability. Irradiation of the local site alone had no effect. T lymphocyte, T lymphoblast, and platelet accumulation generally followed the same pattern as thymocytes. Intravenous injection of neutrophils, but not platelets, partially restored lymphocyte accumulation in vivo in irradiated mice via a pathway involving the circulating neutrophil, and seemed to be independent of changes in vascular permeability.
Hayes
J M
JM
University of Pittsburgh, Pennsylvania.
Simmons
R L
RL
D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D000001:Calcimycin / Q000494:pharmacology*; D002199:Capillary Permeability / Q000187:drug effects; D005260:Female; D007205:Indium Radioisotopes; D007249:Inflammation; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000502:physiology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D013997:Time Factors; D014916:Whole-Body Irradiation
D007205:Indium Radioisotopes; D000001:Calcimycin