title: ATP-citrate lyase is another enzyme the histidine phosphorylation of which is inhibited by vanadate.
abstract: We have recently shown that phosphorylation of histidine residue of the alpha-subunit of the succinyl-CoA synthetase is inhibited by both vanadate and vanadyl. To assess the university of this inhibition, we have estimated the effect of vanadate on the phosphorylation of another enzyme ATP-citrate lyase, prepared from rat liver. This enzyme contains histidine as the only amino acid with an acid-labile (P-N) phosphate bond. The 67% inhibition of endogenous phosphorylation by 1 mM vanadate disappeared after cleavage of the acidic P-N bond of histidine with acidic sample solution. The remaining 33 per cent radioactivity was due to labelling of the acid-stable phosphoamino acids (P-serine and P-threonine), the phosphorylation of which was not affected by vanadate. The dose response curve for vanadate inhibition closely resembles that shown previously for inhibition of phosphorylation of histidine in the succinyl-CoA synthetase. The results suggest that the action of vanadate on histidinyl phosphorylation is a more general effect (like its influence on phosphorylation of the protein-bound tyrosine).
authors.affiliation: Institute of Physiology, Czechoslovak Academy of Sciences, Prague.
authors.forename: J
authors.initials: J
authors.lastname: Krivanek
authors.forename: L
authors.initials: L
authors.lastname: Novakova
mesh_terms: D001275:ATP Citrate (pro-S)-Lyase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000818:Animals; D006639:Histidine / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D010766:Phosphorylation; D051381:Rats; D014638:Vanadates / Q000494:pharmacology*
chemical_list: D014638:Vanadates; D006639:Histidine; D001275:ATP Citrate (pro-S)-Lyase