Analysis of tropane alkaloids with thermospray high-performance liquid chromatography-mass spectrometry.
A thermospray high-performance liquid chromatography-mass spectrometry method for analysis of hyoscyamine and scopolamine in plant cell culture samples is described. The alkaloids were separated on a polymeric reversed-phase column with an alkaline ammonium acetate buffer-acetonitrile eluent. Selected-ion recording of the protonated molecular ions was used for quantitation of the compounds. The compounds were fragmented by discharge-assisted ionization and elevated thermospray capillary temperatures or ion repeller potentials.
Auriola
S
S
Department of Pharmaceutical Chemistry, University of Kuopio, Finland.
Martinsen
A
A
Oksman-Caldentey
K M
KM
Naaranlahti
T
T
D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D010946:Plants, Medicinal / Q000032:analysis; D011786:Quality Control; D012601:Scopolamine / Q000032:analysis; D014326:Tropanes / Q000032:analysis*
D000470:Alkaloids; D014326:Tropanes; C100236:homatropine; D012601:Scopolamine