title: The lysosomal enzymes of the iris-ciliary body are retained in their organelles and exhibit increased activities during acute uveal inflammation.
abstract: Acute uveal inflammation, characterised by conjunctival hyperaemia, limbal vascular injection, iritis and aqueous flare, was induced in the rabbit eye by a single intravitreal injection of 10 micrograms of Shigella endotoxin. Twenty-four, 48 and 72 h after endotoxin administration, lysosomal-rich fractions and cytosolic supernatants were prepared from iris-ciliary bodies and the enzymatic activities of some lysosomal hydrolases in these fractions were assayed. Acid phosphatase, aryl sulphatase and N-acetyl-B-D-glucosaminidase exhibited increased activities in the lysosomal preparations from inflamed iris-ciliary bodies, relative to the activities of the same enzymes in lysosomal fractions from normal iris-ciliary bodies. No significant differences in activities were obtained for these enzymes in cytosolic supernatants from normal and inflamed iris-ciliary bodies. The results suggest that during acute ocular inflammation the lysosomal hydrolases are retained in their organelles at an elevated state of activity.
authors.affiliation: Department of Visual Science, University of London, Great Britain.
authors.forename: A C
authors.initials: AC
authors.lastname: Phylactos
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001192:Arylsulfatases / Q000378:metabolism; D002924:Ciliary Body / Q000201:enzymology*; D003600:Cytosol / Q000201:enzymology; D004731:Endotoxins / Q000008:administration & dosage; D005966:Glucuronidase / Q000378:metabolism; D007498:Iris / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology*; D015388:Organelles / Q000201:enzymology; D011817:Rabbits; D012760:Shigella; D014605:Uveitis / Q000139:chemically induced / Q000201:enzymology*
chemical_list: D004731:Endotoxins; D000135:Acid Phosphatase; D001192:Arylsulfatases; D005966:Glucuronidase; D000118:Acetylglucosaminidase