title: MAP-1 and MAP-2 binding sites at the C-terminus of beta-tubulin. Studies with synthetic tubulin peptides.
abstract: The interaction of microtubule-associated proteins MAP-1 and MAP-2 with different peptides containing sequences covering the C-terminal region of beta-tubulin isoforms has been analyzed. Our results indicate that MAP-1 and MAP-2 bind to a common sequence within the variable C-terminal region of the different beta-tubulin isoforms, while MAP-2 also interacts with the subdomain beta (422-434) of the constant region, in agreement with previous results (Maccioni, R.B., Rivas, C., & Vera, J.C. (1988) EMBO J. 7, 1957-1963). The productive interaction of MAP-2 with the latter domain appears to be involved in the assembly of microtubules.
authors.affiliation: International Center for Cancer and Developmental Biology (ICC), Santiago, Chile.
authors.forename: D
authors.initials: D
authors.lastname: Cross
authors.forename: J
authors.initials: J
authors.lastname: Dominguez
authors.forename: R B
authors.initials: RB
authors.lastname: Maccioni
authors.forename: J
authors.initials: J
authors.lastname: Avila
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D002417:Cattle; D008854:Microscopy, Electron; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D014404:Tubulin / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D008869:Microtubule-Associated Proteins; D014404:Tubulin