Sulfuric acid/hydrogen peroxide digestion and colorimetric a collaborative study.
Seven laboratories participated in a collaborative study of a method for determination of phosphorus in meat and meat products. Samples are digested in sulfuric acid and hydrogen peroxide; digestion is complete in approximately 10 min. Phosphorus is determined by colorimetric analysis of a dilute aliquot of the sample digest. The collaborators analyzed 3 sets of blind duplicate samples from each of 6 classes of meat (U.S. Department of Agriculture classifications): smoked ham, water-added ham, canned ham, pork sausage, cooked sausage, and hamburger. The calibration curve was linear over the range of standard solutions prepared (phosphorus levels from 0.05 to 1.00%); levels in the collaborative study samples ranged from 0.10 to 0.30%. Standard deviations for repeatability (sr) and reproducibility (SR) ranged from 0.004 to 0.012 and 0.007 to 0.014, respectively. Corresponding relative standard deviations (RSDr and RSDR, respectively) ranged from 1.70 to 7.28% and 3.50 to 9.87%. Six laboratories analyzed samples by both the proposed method and AOAC method 24.016 (14th Ed.). One laboratory reported results by the proposed method only. Statistical evaluations indicated no significant difference between the 2 methods. The method has been adopted official first action by AOAC.
Christians
D K
DK
Hach Co., Loveland, CO 80539.
Aspelund
T G
TG
Brayton
S V
SV
Roberts
L L
LL
D000818:Animals; D002417:Cattle; D003124:Colorimetry; D006861:Hydrogen Peroxide; D007202:Indicators and Reagents; D008460:Meat / Q000032:analysis*; D008461:Meat Products / Q000032:analysis*; D010758:Phosphorus / Q000032:analysis*; D013055:Spectrophotometry, Infrared; D013464:Sulfuric Acids; D013552:Swine
D007202:Indicators and Reagents; D013464:Sulfuric Acids; D010758:Phosphorus; D006861:Hydrogen Peroxide