Intracerebral transfer of an in vitro established microglial cell line: local induction of a protective state against lethal challenge with Candida albicans.
An in vitro generated BV-2 microglial cell line has been transferred intracerebrally into syngeneic immunocompetent mice prior to local challenge with Candida albicans. The transfer resulted in the establishment of local protection against a lethal dose of C. albicans, which was accompanied by an impairment of yeast growth in the brain and kidneys. Upon histological examination of brain sections from BV-2 cell-pretreated mice, it was found that the size and number of granulomas was reduced as compared to untreated controls receiving Candida alone. These observations provide direct evidence that microglia play a crucial role in the local defense against intracerebral infections.
Blasi
E
E
Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
Mazzolla
R
R
Barluzzi
R
R
Mosci
P
P
Bartoli
A
A
Bistoni
F
F
D000818:Animals; D001921:Brain / Q000473:pathology / Q000502:physiology*; D002177:Candidiasis / Q000473:pathology / Q000517:prevention & control*; D002460:Cell Line; D006412:Hematopoietic Stem Cells / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D009457:Neuroglia / Q000502:physiology* / Q000637:transplantation