title: Glass encodes a site-specific DNA-binding protein that is regulated in response to positional signals in the developing Drosophila eye.
abstract: The glass gene encodes a zinc finger protein required for normal photoreceptor cell development in Drosophila. We show that glass transcripts are present in the third-instar eye-imaginal disc starting in the morphogenetic furrow and extending to the posterior margin of the disc; glass protein is detected in the nuclei of all cells in this region. We also show that glass encodes a site-specific DNA-binding protein. A 27-bp glass-binding site can confer glass-dependent expression on a reporter gene in developing photoreceptor cells, the particular subset of glass-expressing cells known to require glass function. This specificity may represent a regulation of glass protein activity after cells are recruited to the photoreceptor cell fate.
authors.affiliation: Howard Hughes Medical Institute, University of California, Berkeley 94720.
authors.forename: K
authors.initials: K
authors.lastname: Moses
authors.forename: G M
authors.initials: GM
authors.lastname: Rubin
mesh_terms: D000818:Animals; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics*; D004330:Drosophila / Q000235:genetics / Q000254:growth & development*; D004742:Enhancer Elements, Genetic; D005123:Eye / Q000254:growth & development; D005796:Genes; D007814:Larva; D008969:Molecular Sequence Data; D009024:Morphogenesis; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D010786:Photoreceptor Cells / Q000502:physiology; D016335:Zinc Fingers / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D015345:Oligonucleotide Probes