Relationship between gliadin peptide structure and their effect on the fetal chick duodenum.
The tendency to form a beta-turn in alpha-gliadin was estimated using the B-cell determinant prediction program based on the Chou and Fasman probability of beta-turn formation. Six sequences possessing a high probability of beta-turn formation were found. A statistically high agreement was found between these six sequences and three areas in alpha-gliadin with the occurrence of Pro-Ser-Gln-Gln sequence which has recently been considered responsible for toxicity in coeliac disease. By means of solid-phase synthesis seven peptides were obtained covering the above-mentioned regions. Their toxicity was tested using the fetal chick duodenum. The results support the suggestion that peptides containing the sequences Pro-Ser-Gln-Gln and Gln-Gln-Gln-Pro may be involved in the pathogenesis of coeliac disease.
Kocna
P
P
Laboratory of Gastroenterology, Faculty of General Medicine, Charles University, Prague, Czechoslovakia.
Mothes
T
T
Krchnák
V
V
Fric
P
P
D000595:Amino Acid Sequence; D000818:Animals; D002446:Celiac Disease / Q000209:etiology*; D002642:Chick Embryo; D004386:Duodenum / Q000187:drug effects* / Q000196:embryology; D005903:Gliadin / Q000737:chemistry* / Q000633:toxicity; D008969:Molecular Sequence Data
D005903:Gliadin