Three-dimensional readout of flash x-ray images of living sperm in water by atomic-force microscopy.
The imaging of living specimens in water by x-ray microscopy can be greatly enhanced with the use of an intense flash x-ray source and sophisticated technologies for reading x-ray images. A subnanosecond [corrected] x-ray pulse from a laser-produced plasma was used to record the x-ray image of living sea urchin sperm in an x-ray resist. The resist relief was visualized at high resolution by atomic-force microscopy. Internal structure of the sperm head was evident, and the carbon density in a flagellum was estimated from the relief height.
Tomie
T
T
Electrotechnical Laboratory, Ibaraki, Japan.
Shimizu
H
H
Majima
T
T
Yamada
M
M
Kanayama
T
T
Kondo
H
H
Yano
M
M
Ono
M
M
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D008297:Male; D008768:Methylmethacrylates; D008853:Microscopy / Q000379:methods*; D008928:Mitochondria / Q000648:ultrastructure; D012617:Sea Urchins; D012623:Seawater; D013082:Sperm Tail / Q000648:ultrastructure*; D014965:X-Rays
D008768:Methylmethacrylates