title: Twelve-hour cardiopulmonary preservation using donor core cooling, leukocyte depletion, and liposomal superoxide dismutase.
abstract: Because leukocytes and oxygen radical species contribute to ischemic and reperfusion injury during organ preservation, we examined the effects of a long-acting liposomal superoxide dismutase (liposomal SOD) and mechanical filtration of leukocytes on cardiopulmonary graft function after 12 hours of static preservation. Bovine heart-lung blocks were harvested, core cooled to 15 degrees C, stored in 4 degrees C donor blood for 12 hours, and then orthotopically transplanted (control group, n = 6). In the leukocyte-depletion group (n = 6), a leukocyte filter was incorporated in the bypass circuits of the donor and recipient. In the SOD group (n = 6), liposomal SOD (5000 U/kg) was administered in the cardioplegic solution, in the prime of the bypass circuits of donor and recipient, and immediately before recipient heart-lung reperfusion. In the combination group (n = 6), both leukocyte depletion (LD) and liposomal SOD were used. Only four of six control animals survived more than 2 hours after weaning from bypass, whereas all LD, SOD, and LD + SOD animals survived to be studied at 6 hours. Pulmonary function was assessed at 6 hours by arterial oxygen tension on 100% inspired oxygen (PO2), pulmonary vascular resistance (PVR), and postmortem wet/dry lung weight ratios. Arterial pO2 values (mm Hg) were as follows: control, 102 +/- 51; LD, 437 +/- 60*; SOD, 278 +/- 83; and LD + SOD, 504 +/- 54* (*p less than 0.05 vs controls). PVR values (dynes . sec . cm5) were as follows: control, 1975 +/- 697; LD, 682 +/- 131*; SOD, 607 +/- 191*; and LD + SOD 367 +/- 87* (*p less than 0.05 vs controls).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Division of Cardiac Surgery, Johns Hopkins Medical Institution, Baltimore, Md.
authors.forename: K
authors.initials: K
authors.lastname: Bando
authors.forename: S
authors.initials: S
authors.lastname: Schueler
authors.forename: D E
authors.initials: DE
authors.lastname: Cameron
authors.forename: P A
authors.initials: PA
authors.lastname: DeValeria
authors.forename: M
authors.initials: M
authors.lastname: Hatanaka
authors.forename: A S
authors.initials: AS
authors.lastname: Casale
authors.forename: M A
authors.initials: MA
authors.lastname: Zebley
authors.forename: G M
authors.initials: GM
authors.lastname: Hutchins
authors.forename: B A
authors.initials: BA
authors.lastname: Reitz
authors.forename: W A
authors.initials: WA
authors.lastname: Baumgartner
mesh_terms: D000818:Animals; D002314:Cardioplegic Solutions; D002417:Cattle; D006321:Heart; D016041:Heart-Lung Transplantation; D008081:Liposomes; D008168:Lung; D008212:Lymphocyte Depletion; D009926:Organ Preservation / Q000379:methods*; D013482:Superoxide Dismutase / Q000008:administration & dosage; D013997:Time Factors
chemical_list: D002314:Cardioplegic Solutions; D008081:Liposomes; D013482:Superoxide Dismutase