Blood flow stasis induced by cw-Nd:YAG laser irradiation: comparative morphology of mesenteric and choroidal vessels in pigmented rabbits.
cw-Nd:YAG laser radiation was effective in producing blood flow stasis within arteries (35-40 microns in diameter) of pigmented rabbit mesentery (beam spot size: 80 microns; fluence: 2 x 10(4) J cm-2) and choroid (beam spot size: 200 microns; fluence: 6 x 10(2) J cm-2). However, the mechanism by which this was achieved differed in each case, and depended upon the energy absorbing structures present in the irradiated tissue. In non-pigmented tissue, such as the mesentery, haemoglobin represents the primary absorption centre, and the temperature attained within the vessel lumen (as inferred from morphological changes) is sufficient to denature plasma proteins, in particular fibrinogen, which consequently occlude the vessel lumen and arrest bleeding. In pigmented tissue, such as the choroid, melanocyte granules represent the primary absorption centre, which is thus shifted from the vessel lumen to the stroma. The temperature rise achieved within the vessel lumen is consequently lower, as evidenced by the absence of plasma protein denaturation. Blood flow stasis nonetheless occurs, but is triggered according to the normal haemostatic mechanism.
van der Zypen
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Institute of Anatomy, University of Bern, Switzerland.
England
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C
Fankhauser
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F
Kwasniewska
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S
D000818:Animals; D001158:Arteries / Q000528:radiation effects / Q000648:ultrastructure; D002196:Capillaries / Q000528:radiation effects / Q000648:ultrastructure; D002829:Choroid / Q000098:blood supply*; D006487:Hemostasis / Q000528:radiation effects*; D053685:Laser Therapy; D008638:Mesenteric Arteries / Q000528:radiation effects* / Q000648:ultrastructure; D011817:Rabbits; D012880:Skin Pigmentation / Q000502:physiology