Physical map of the Saccharomyces cerevisiae genome at 110-kilobase resolution.
A physical map of the Saccharomyces cerevisiae genome is presented. It was derived by mapping the sites for two restriction endonucleases, SfiI and NotI, each of which recognizes an 8-bp sequence. DNA-DNA hybridization probes for genetically mapped genes and probes that span particular SfiI and NotI sites were used to construct a map that contains 131 physical landmarks--32 chromosome ends, 61 SfiI sites and 38 NotI sites. These landmarks are distributed throughout the non-rDNA component of the yeast genome, which comprises 12.5 Mbp of DNA. The physical map suggests that those genes that can be detected and mapped by standard genetic methods are distributed rather uniformly over the full physical extent of the yeast genome. The map has immediate applications to the mapping of genes for which single-copy DNA-DNA hybridization probes are available.
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A J
AJ
Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110.
Olson
M V
MV
D015139:Blotting, Southern; D002842:Chromatids; D002874:Chromosome Mapping / Q000379:methods; D015825:Chromosomes, Fungal; D015342:DNA Probes; D004271:DNA, Fungal / Q000235:genetics*; D004586:Electrophoresis; D005819:Genetic Markers; D007621:Karyotyping; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic / Q000235:genetics; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D015342:DNA Probes; D004271:DNA, Fungal; D005819:Genetic Markers