title: Computer studies on the stereostructure and quantum chemical properties of 6-pyruvoyl tetrahydropterin, the key intermediate of tetrahydrobiopterin biosynthesis.
abstract: The optimized geometry of the conformation of atoms constituting the 6-pyruvoyl tetrahydropterin molecule, the labile key intermediate of tetrahydrobiopterin biosynthesis, was obtained by molecular orbital calculations within the MINDO/3 framework. The stereostructure of the molecule showing the preferred mode for binding to sepiapterin reductase or pyruvoyl tetrahydropterin reductase was drawn in perspective. The resulting structure with the equatorial staggered configuration of the 6-1',2'-dioxopropyl (pyruvoyl) side chain indicated that O(1') and H(6) were located in the trans position around the C(6)-C(1') bond and that the two vicinal carbonyls in the side chain were fixed in the incomplete trans form. The calculation of atomic charges and LUMO coefficients of these carbonyls suggests that the C2'-carbonyl may be more reactive toward NADPH than the C1'-carbonyl in the enzymatic reaction.
authors.affiliation: Department of Biochemistry, Meikai University School of Dentistry, Saitama, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Katoh
authors.forename: T
authors.initials: T
authors.lastname: Sueoka
authors.forename: T
authors.initials: T
authors.lastname: Kurihara
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism; D001708:Biopterins / Q000031:analogs & derivatives* / Q000096:biosynthesis; D003198:Computer Simulation; D007658:Ketone Oxidoreductases / Q000378:metabolism; D008958:Models, Molecular; D008968:Molecular Conformation; D015394:Molecular Structure; D011622:Pterins / Q000737:chemistry* / Q000378:metabolism; D011789:Quantum Theory
chemical_list: D011622:Pterins; D001708:Biopterins; C045652:dyspropterin; D000429:Alcohol Oxidoreductases; C022630:sepiapterin reductase; D007658:Ketone Oxidoreductases; C059712:6-pyruvoyl tetrahydropterin (2'-oxo)reductase; C003402:sapropterin