Polyclonal antibodies to human milk caseins.
Preparations of total human casein and its individual fractions were isolated for production of specific polyclonal antibodies. Immunization procedures used differed in the schedule for antigen administration, antigen concentrations, degree of additional purification, and modification of the size of molecules. Immunoprecipitation techniques failed to provide an unambiguous proof of the presence of antibodies to human milk caseins in antisera even after immunization of animals phylogenetically considerably different from man. Immunoblotting, however, allowed antibodies to beta-casein to be identified and compared with available monoclonal antibodies.
Simícková
M
M
Institutes of Medical Research, Research Institute of Clinical and Experimental Oncology, Brno, Czechoslovakia.
Lang
B A
BA
Slepicka
L
L
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000917:Antibody Formation; D002364:Caseins / Q000032:analysis / Q000276:immunology*; D005591:Chemical Fractionation; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D015151:Immunoblotting; D007122:Immunoelectrophoresis; D008895:Milk, Human / Q000276:immunology*; D011233:Precipitin Tests; D011817:Rabbits; D012756:Sheep; D014422:Turkeys
D000906:Antibodies; D002364:Caseins; D007106:Immune Sera