Thyroidal iodine metabolism in obese-strain chickens before immune-mediated damage.
Several studies have shown that iodine plays a role in spontaneous autoimmune thyroiditis in man and other animals. In addition, abnormalities of iodine metabolism have been found in patients with Hashimoto's thyroiditis and in chickens of the obese strain (OS), an animal model of spontaneous autoimmune thyroiditis. We have examined several parameters of iodine metabolism before immune damage in this model and in the related Cornell strain (CS), a strain which develops a late-onset mild thyroiditis, to discover a possible causal relationship between altered iodine metabolism and the initiation of autoimmunity. Thyroglobulin was purified from individual chicken thyroid glands and analysed for iodine by the ceric sulphate method. Analogous to the thyroglobulin of Hashimoto's patients, the iodine content of OS thyroglobulin (27 atoms/molecule) was lower than that of normal-strain thyroglobulin (46 atoms/molecule) when the chickens were provided with a normal diet. Also, under conditions of TSH suppression, the iodine content of OS thyroglobulin (18 atoms/molecule) was lower than that of CS thyroglobulin (36 atoms/molecule) and of normal-strain thyroglobulin (32 atoms/molecule). In contrast with Hashimoto's patients, however, the OS and CS chickens had practically no inorganic iodide in their thyroid glands; electrophoretic analysis of thyroid homogenates revealed that essentially all (greater than 99.62%) 125I was organified by 16 h in all strains of birds tested. Despite the relatively poor iodination of thyroglobulin exhibited by OS chickens, they did not iodinate additional 'unique' proteins, when examined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of thyroid proteins labelled with 125I in vivo.(ABSTRACT TRUNCATED AT 250 WORDS)
Sundick
R S
RS
Department of Immunology and Microbiology, Wayne State University, Detroit, Michigan 48201.
Herdegen
D
D
Brown
T R
TR
Dhar
A
A
Bagchi
N
N
D000818:Animals; D015551:Autoimmunity / Q000502:physiology*; D001345:Autoradiography; D002645:Chickens / Q000378:metabolism*; D004195:Disease Models, Animal; D007455:Iodine / Q000032:analysis / Q000378:metabolism*; D009765:Obesity / Q000378:metabolism* / Q000503:physiopathology; D013954:Thyroglobulin / Q000032:analysis; D013961:Thyroid Gland / Q000187:drug effects / Q000378:metabolism* / Q000503:physiopathology; D013967:Thyroiditis, Autoimmune / Q000378:metabolism; D013974:Thyroxine / Q000494:pharmacology
D013954:Thyroglobulin; D007455:Iodine; D013974:Thyroxine