GCDFP-70 protein in cyst fluid identified as albumin and used to classify cysts in women with breast gross cystic disease.
We used sodium dodecyl sulfate-polyacrylamide gel electrophoresis to study cyst fluids from women with breast gross cystic disease. The subjects could be classified into two categories according to the concentrations of protein GCDFP-70 in the cyst fluid: those with Type I cysts had a very low content of this protein; those with Type II cysts had very high concentrations. Analysis of the amino acid sequence of GCDFP-70 from both cyst fluid types confirmed that this protein is human plasma albumin. The average concentration of albumin found in Type I cyst fluids was 0.32 g/L and that corresponding to Type II was 10.16 g/L. Thus, albumin quantification from cyst fluids or analysis by either polyacrylamide or agarose gel electrophoresis provides a simple procedure for classifying these fluids, yielding results that correlate well with previous classifications based on other measurements such as sodium, potassium, and chloride concentrations. This albumin-based quantification method may improve the classification of breast cysts and might be useful in further studies on functional changes in the cysts and their relationship to breast cancer.
Balbín
M
M
Departamento de Biología Funcional, Facultad de Medicina, Universidad de Oviedo, Spain.
Vizoso
F
F
Sánchez
L M
LM
Venta
R
R
Ruibal
A
A
Fueyo
A
A
López-Otín
C
C
D000328:Adult; D000595:Amino Acid Sequence; D001053:Apolipoproteins; D053399:Apolipoproteins D; D002352:Carrier Proteins / Q000032:analysis* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005122:Exudates and Transudates / Q000378:metabolism; D005260:Female; D005348:Fibrocystic Breast Disease / Q000145:classification* / Q000378:metabolism; D006023:Glycoproteins; D006801:Humans; D026901:Membrane Transport Proteins; D008875:Middle Aged; D008969:Molecular Sequence Data; D012709:Serum Albumin / Q000032:analysis* / Q000302:isolation & purification
C024328:APOD protein, human; D001053:Apolipoproteins; D053399:Apolipoproteins D; D002352:Carrier Proteins; D006023:Glycoproteins; D026901:Membrane Transport Proteins; D012709:Serum Albumin