Modified method of mammalian cell synchronization improves yield and degree of synchronization.
A modified method to synchronize CHO and HeLa cells is developed based upon a combined shaking-off and chemical blockage. This method has effectively blocked quiescent cells, which is the main obstacle of high degree synchronization. Flow-cytometry data show the improvement on the degree of synchronization and yield compared to two previously used methods.
Cao
G
G
Physiology Department, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
Liu
L M
LM
Cleary
S F
SF
D000818:Animals; D002448:Cell Adhesion; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured / Q000166:cytology*; D006224:Cricetinae; D003412:Cricetulus; D006367:HeLa Cells; D006918:Hydroxyurea / Q000494:pharmacology; D013936:Thymidine / Q000494:pharmacology
D013936:Thymidine; D006918:Hydroxyurea