Immunofluorescent microscopical visualization of trails left by gliding Cryptosporidium parvum sporozoites.
Cryptosporidium parvum sporozoites that exhibited gliding motility in vitro were examined by immunofluorescence with anticryptosporidial monoclonal antibodies (Mabs) for surface antigen deposition on poly-L-lysine-coated glass microscope slides. The Mabs that revealed trails are specific for an immunodominant 23-kDa antigen previously localized to the sporozoite surface.
Arrowood
M J
MJ
Department of Animal Science, Utah State University, Logan 84322-5600.
Sterling
C R
CR
Healey
M C
MC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000953:Antigens, Protozoan / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis; D003458:Cryptosporidium / Q000276:immunology / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D009068:Movement
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000954:Antigens, Surface