Carcinogenicity study of fecapentaene-12 diacetate on skin painting in SENCAR mice.
To investigate the effects of both diol esterification and coadministration with antioxidant on the tumorigenicity of fecapentaene-12 (FP-12) preparations, diacetylfecapentaene-12 (DAFP-12) in dimethylsulfoxide (DMSO) was applied to SENCAR mouse skin with or without the stabilizer, vitamin E, twice/week for 5 weeks, following which all animals were promoted for up to 25 weeks by weekly applications of 12-O-tetradecanoylphorbol-13-acetate (TPA). While positive controls receiving 7,12-dimethylbenz[a]anthracene (DMBA) instead of DAFP-12 in a similar protocol all developed papillomas (average of 23/animal), papilloma incidence in mice given DAFP-12 did not differ significantly from that of the vehicle control. We conclude that DAFP-12 shows little or no tumor initiating activity for mouse skin even when coadministered with vitamin E.
Devor
D E
DE
Laboratory of Comparative Carcinogenesis, National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702.
Henneman
J R
JR
Keefer
L K
LK
Logsdon
D L
DL
Rice
J M
JM
Streeter
A J
AJ
Ward
J M
JM
D000328:Adult; D000818:Animals; D004338:Drug Combinations; D005260:Female; D006801:Humans; D051379:Mice; D009153:Mutagens / Q000494:pharmacology*; D010212:Papilloma / Q000139:chemically induced*; D011090:Polyenes / Q000009:adverse effects* / Q000494:pharmacology*; D012878:Skin Neoplasms / Q000139:chemically induced*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology
D004338:Drug Combinations; D009153:Mutagens; D011090:Polyenes; C067887:diacetylfecapentaene-12; D014810:Vitamin E; C037357:1-(1-glycero)dodeca-1,3,5,7,9-pentaene; D013755:Tetradecanoylphorbol Acetate