The role of apoE in inhibitory effects of apoE-rich HDL on platelet function.
Apolipoprotein E- (ApoE-) rich high-density lipoprotein (HDL), which was prepared from the bound fraction of normolipemic volunteers on heparin-Sepharose and from a hyperalphalipoproteinemic patient, potently inhibited aggregation of human platelets in a dose-dependent fashion. Dimyristoyl phosphatidylcholine liposome with apoE (apoE.DMPC) also inhibited platelet aggregation, and incubation of washed platelets with apoE.DMPC resulted in the release of cholesterol into the supernatant in a time- and dose-dependent manner. These results suggest that apoE plays a major role in the inhibitory effect of apoE-rich HDL in platelet function, presumably due to the release of cholesterol from the plasma membrane.
Higashihara
M
M
First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Kinoshita
M
M
Teramoto
T
T
Kume
S
S
Kurokawa
K
K
D000255:Adenosine Triphosphate / Q000378:metabolism; D001057:Apolipoproteins E / Q000378:metabolism / Q000502:physiology*; D002784:Cholesterol / Q000378:metabolism; D003094:Collagen / Q000037:antagonists & inhibitors; D004134:Dimyristoylphosphatidylcholine / Q000378:metabolism; D006801:Humans; D006951:Hyperlipoproteinemias / Q000097:blood; D007700:Kinetics; D008075:Lipoproteins, HDL / Q000502:physiology*; D010974:Platelet Aggregation; D013917:Thrombin / Q000037:antagonists & inhibitors
D001057:Apolipoproteins E; D008075:Lipoproteins, HDL; D000255:Adenosine Triphosphate; D003094:Collagen; D002784:Cholesterol; D013917:Thrombin; D004134:Dimyristoylphosphatidylcholine