title: Development of specific antisera against 9-desglycinamide-8-arginine vasopressin by site-specific immunization.
abstract: Four specific and sensitive antisera against 9-desglycinamide-8-arginine vasopressin (DGAVP) were obtained by means of site-specific immunization in rabbits, using a Keyhole Limpet haemocyanin-DGAVP conjugate synthesized by a specific reaction of a thiol function with a maleimide group. Compared with conventionally raised antisera, the cross-reactivity with endogenous arginine vasopressin (AVP) and vasopressin-derived fragments was substantially lower. Interassay variability was 18.1% at maximum. Possible applicability of the new antisera in pharmacokinetic studies of DGAVP with respect to blood-brain barrier transport was tested by means of a pilot study in the rat.
authors.affiliation: Division of Pharmacology, Leiden University, The Netherlands.
authors.forename: J B
authors.initials: JB
authors.lastname: van Bree
authors.forename: J W
authors.initials: JW
authors.lastname: van Nispen
authors.forename: J C
authors.initials: JC
authors.lastname: Verhoef
authors.forename: D D
authors.initials: DD
authors.lastname: Breimer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity / Q000276:immunology; D001127:Arginine Vasopressin / Q000031:analogs & derivatives* / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology; D005260:Female; D007106:Immune Sera / Q000096:biosynthesis* / Q000276:immunology; D007114:Immunization / Q000379:methods*; D008969:Molecular Sequence Data; D011817:Rabbits; D015203:Reproducibility of Results
chemical_list: D007106:Immune Sera; D001127:Arginine Vasopressin; C015119:argipressin, des-GlyNH2(9)-