Transcriptional start and MetR binding sites on the Escherichia coli metH gene.
The 5' upstream region of the Escherichia coli metH gene has been sequenced. Primer extension analysis revealed a transcription start site at 324 bases upstream of the initiator codon. An 8 base sequence homologous to the MetR binding region on the E. coli metE gene is present 217 bp downstream of the transcription start site. Gel retardation experiments showed that purified MetR protein could bind to a 30 base oligonucleotide containing the putative MetR binding region. No "met box" was present which explains the relative lack of regulation of the expression of the metH gene by methionine.
Marconi
R
R
Roche Research Center, Nutley, NJ 07110.
Wigboldus
J
J
Weissbach
H
H
Brot
N
N
D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / Q000235:genetics*; D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D008715:Methionine / Q000096:biosynthesis; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010957:Plasmids; D015534:Trans-Activators / Q000378:metabolism*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C058169:MetR protein, Bacteria; D015345:Oligonucleotide Probes; D015534:Trans-Activators; D008715:Methionine; D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase