title: Nucleotide sequence analysis of a chloramphenicol-resistance determinant from Agrobacterium tumefaciens and identification of its gene product.
abstract: The nucleotide sequence of a chloramphenicol-resistance (CmR) determinant from the Gram- soil bacterium Agrobacterium tumefaciens was determined, and its gene product was identified as Cm acetyltransferase (CAT). Comparison of the amino acid sequences of the A. tumefaciens CAT and various CAT proteins of Gram+ and Gram- origin shows no homology between this and the other enzymes.
authors.affiliation: Molekulare Genetik der Universität Heidelberg, F.R.G.
authors.forename: J
authors.initials: J
authors.lastname: Tennigkeit
authors.forename: H
authors.initials: H
authors.lastname: Matzura
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics* / Q000378:metabolism; D002702:Chloramphenicol Resistance / Q000235:genetics*; D002855:Chromatography, Thin Layer; D004269:DNA, Bacterial; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012231:Rhizobium / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004269:DNA, Bacterial; D015500:Chloramphenicol O-Acetyltransferase