title: Bone-inducing activity of mature BMP-2b produced from a hybrid BMP-2a/2b precursor.
abstract: The human osteoinductive proteins BMP-2a and BMP-2b have been cloned and expressed in mammalian cells. In order to improve expression levels we examined the role of the proregion in assembly and export. Use of the BMP-2a proregion combined with the mature region of BMP-2b leads to dramatically improved expression of mature BMP-2b. Mature BMP-2b has been purified to near homogeneity from the BMP-2a/2b hybrid, and its structural properties and biological activity determined. Recombinant mature BMP-2b homodimer elicits bone formation in vivo.
authors.affiliation: Department of Developmental Biology, Genentech, Inc., South San Francisco, California 94080.
authors.forename: R G
authors.initials: RG
authors.lastname: Hammonds
authors.forename: R
authors.initials: R
authors.lastname: Schwall
authors.forename: A
authors.initials: A
authors.lastname: Dudley
authors.forename: L
authors.initials: L
authors.lastname: Berkemeier
authors.forename: C
authors.initials: C
authors.lastname: Lai
authors.forename: J
authors.initials: J
authors.lastname: Lee
authors.forename: N
authors.initials: N
authors.lastname: Cunningham
authors.forename: A H
authors.initials: AH
authors.lastname: Reddi
authors.forename: W I
authors.initials: WI
authors.lastname: Wood
authors.forename: A J
authors.initials: AJ
authors.lastname: Mason
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001846:Bone Development / Q000187:drug effects*; D019485:Bone Morphogenetic Proteins; D001842:Bone and Bones / Q000187:drug effects; D002460:Cell Line; D003001:Cloning, Molecular; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics*; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000494:pharmacology; D014162:Transfection
chemical_list: D019485:Bone Morphogenetic Proteins; D011498:Protein Precursors; D011506:Proteins; D011993:Recombinant Fusion Proteins