Age-related changes in release of soluble interleukin-2 receptor by murine lymphocytes.
The purpose of this study was to investigate whether the presence of soluble interleukin-2 receptors (sIL-2R) in the supernatants of activated splenocytes correlates with age progression in C57BL/6 mice. In addition, the relationship between IL-2R cell surface expression and release of sIL-2R was examined. The results indicated splenocytes from young (3-4-month-old) mice release higher levels of sIL-2R than those from intermediate (18-19-month-old) or old (24-25-month-old) mice. While there was no statistical difference between sIL-2R levels in intermediate and old mice in this study, the old mice did have a slightly higher release of sIL-2R than the intermediate mice. There was a correlation between IL-2R cell surface expression and the level of sIL-2R in the young and the intermediate age groups. In old mice there was no correlation between these two parameters.
Kariv
I
I
Pennsylvania State University, Department of Veterinary Science, University Park 16802.
Sharlow
E
E
Garman
C
C
Ferguson
F
F
D000375:Aging / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal; D003208:Concanavalin A / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis*; D013154:Spleen / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D015375:Receptors, Interleukin-2; D003208:Concanavalin A; D013755:Tetradecanoylphorbol Acetate