Progress in immunoassays for serum prolactin: evidence from the UK External Quality Assessment Scheme (EQAS) 1980-1989.
The quality of serum prolactin assays routinely performed by UK laboratories has been monitored in an external quality assessment scheme (EQAS) over a 10-year period, during which participation in the EQAS increased three-fold, and considerable changes in methods and standardization were introduced. The all-laboratory mean was used as the sample target value, and proved to be stable and accurate. Overall between-laboratory agreement in the clinically important range improved from a geometric coefficient of variation (GCV) of 25% to 14%. This appears to reflect the increased use of kits in place of 'in-house' assays, the more widespread availability of international standards and the absence of any marked differences in bias between the commonly used methods. Published guidelines on the clinical interpretation of prolactin values should, therefore, be widely applicable. The EQAS data indicate that, in general, the quality of performance of prolactin assays is adequate for their clinical application.
Hanning
I
I
Department of Clinical Chemistry, University of Edinburgh, UK.
Seth
J
J
Bacon
R R
RR
Hunter
W M
WM
al-Sadie
R
R
D000911:Antibodies, Monoclonal; D006301:Health Services Needs and Demand; D006801:Humans; D007118:Immunoassay / Q000592:standards*; D011388:Prolactin / Q000097:blood*; D011785:Quality Assurance, Health Care; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D006113:United Kingdom
D000911:Antibodies, Monoclonal; D011933:Reagent Kits, Diagnostic; D011388:Prolactin