Acquisition and loss of a neuronal Ca2+/calmodulin-dependent protein kinase during neuronal differentiation.
Calcium ions play a critical role in neural development. Insights into the ontogeny of Ca(2+)-signaling pathways were gained by investigating the developmental expression of granule cell-enriched Ca2+/calmodulin-dependent protein kinase (CaM kinase-Gr) in the cerebellum and hippocampus of the rat. Neurons of these brain regions displayed characteristic schedules by which they acquired and lost CaM kinase-Gr during differentiation. In the cerebellum, granule cells did not begin to express CaM kinase-Gr until after birth when they migrated into the granule cell layer, and this expression persisted in the adult. Purkinje cells expressed CaM kinase-Gr prenatally and lost this expression by postnatal day 14. In contrast, the granule and pyramidal cells of the hippocampus expressed the enzyme prenatally and in the adult. Moreover, CaM kinase-Gr was localized to the processes and nuclei of developing neurons. This subcellular localization together with the scheduled expression of CaM kinase-Gr can serve to regulate a developing neuron's sensitivity to Ca2+ at different subcellular levels.
Jensen
K F
KF
Neurotoxicology Division, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711.
Ohmstede
C A
CA
Fisher
R S
RS
Olin
J K
JK
Sahyoun
N
N
D000818:Animals; D015153:Blotting, Western; D002118:Calcium / Q000502:physiology; D002147:Calmodulin / Q000502:physiology; D002454:Cell Differentiation; D002531:Cerebellum / Q000166:cytology / Q000201:enzymology; D006624:Hippocampus / Q000201:enzymology; D009474:Neurons / Q000166:cytology / Q000201:enzymology*; D011494:Protein Kinases / Q000276:immunology / Q000378:metabolism*; D011689:Purkinje Cells / Q000201:enzymology; D051381:Rats
D002147:Calmodulin; D011494:Protein Kinases; D002118:Calcium