Rapid polymerase chain reaction amplification using intact bacterial cells.
We have demonstrated that efficient polymerase chain reaction amplifications from chromosomal DNA can be carried out using whole bacterial cells as the starting material. Cells from the liquid or solid cultures can be used directly, without any pre-treatment, thus eliminating the need for DNA isolation.
Joshi
A K
AK
Division of Biochemistry and Molecular Biology, University of California, Berkeley 94720.
Baichwal
V
V
Ames
G F
GF
D018528:ATP-Binding Cassette Transporters; D026923:Amino Acid Transport Systems, Basic; D001426:Bacterial Proteins; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D026901:Membrane Transport Proteins / Q000235:genetics; D009876:Operon; D016133:Polymerase Chain Reaction / Q000379:methods*; D012486:Salmonella typhimurium / Q000235:genetics*
D018528:ATP-Binding Cassette Transporters; D026923:Amino Acid Transport Systems, Basic; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D026901:Membrane Transport Proteins; C043828:histidine permease, Bacteria