A novel 57 kDa peroxisomal membrane polypeptide detected by monoclonal antibody (PXM1a/207B).
BALB/c mice were immunized with peroxisomal membranes prepared from rat liver. Spleen cells were fused with myeloma cells (P3/U1) and the hybridomas were selected using peroxisomal membranes. A monoclonal antibody (PXM1a/207B) which recognized peroxisomal membranes was selected. Using the antibody, a novel 57 kDa polypeptide was identified in the peroxisomal membrane fraction. Immunoblot analysis of the subcellular fractions demonstrated that the 57 kDa polypeptide was present exclusively in peroxisomal membranes. The 57 kDa polypeptide was partially digested by trypsin and chymotrypsin under conditions where peroxisomal particles remained intact, indicating that the polypeptide is exposed to the cytosolic face of the peroxisomal membrane. The amount of 57 kDa polypeptide increased in parallel with proliferation of peroxisomes by administration of clofibrate.
Imanaka
T
T
Department of Microbiology and Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Lazarow
P B
PB
Takano
T
T
D000818:Animals; D000911:Antibodies, Monoclonal; D002458:Cell Fractionation / Q000379:methods; D005260:Female; D015151:Immunoblotting; D007425:Intracellular Membranes / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D008099:Liver / Q000737:chemistry / Q000648:ultrastructure; D008565:Membrane Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008830:Microbodies / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D008970:Molecular Weight; D051381:Rats
D000911:Antibodies, Monoclonal; D008565:Membrane Proteins