Hair analysis for drug abuse: I. Determination of methamphetamine and amphetamine in hair by stable isotope dilution gas chromatography/mass spectrometry method.
Determination of methamphetamine and amphetamine in hair was performed by gas chromatography/mass spectrometry using stable isotope-labeled internal standards, 2-methylamino-1-phenylpropane-2,3,3,3-d4 and 2-amino-1-phenylpropane-2,3,3,3-d4. Extraction of hair with methanol/5M hydrochloric acid (20:1) using ultrasonication was chosen as the standard method. The calibration curves for amphetamines in the hair were linear from 1 to 100 ng/mg (r greater than 0.99). The detection limit was 0.5 ng/mg at the 95% confidence level. The coefficients of variation (CV) (n = 8) of analysis using the spiked hair with methamphetamine were from 0.7 to 6%. The CV (n = 8) of analysis of the methamphetamine abuser's hair was 17.5%. Sectional analysis of monkey and human hair after methamphetamine ingestion suggested a good correlation between the duration of drug use and drug distribution in the hair.
Nakahara
Y
Y
Narcotics Section, National Institute of Hygiene Sciences, Tokyo, Japan.
Takahashi
K
K
Shimamine
M
M
Takeda
Y
Y
D000328:Adult; D000661:Amphetamine / Q000032:analysis* / Q000302:isolation & purification; D000818:Animals; D002138:Calibration; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006197:Hair / Q000737:chemistry*; D006801:Humans; D008252:Macaca fascicularis; D008297:Male; D008694:Methamphetamine / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results; D013048:Specimen Handling; D019966:Substance-Related Disorders / Q000175:diagnosis*
D008694:Methamphetamine; D000661:Amphetamine