title: Transforming growth factor beta expression in human marrow stromal cells.
abstract: The effects of hematopoietic cytokines on the expression of transforming growth factors (TGF beta) mRNA and the effect of TGF beta on cytokine and on a major extracellular matrix protein, collagen I, mRNA expression was studied in human marrow stromal cells. As with other cultured mesenchymal cells, stromal cells constitutively express TGF beta 1 but not TGF alpha mRNA. In simian virus 40 (SV40)-transformed stromal cells downregulation of TGF beta 1 expression was observed 2 hours after incubation with recombinant human (rh) tumor-necrosis factor alpha (TNF alpha) and 144 h after addition of rh granulocyte macrophage colony-stimulating factor (GM-CSF). Neither interleukin-1 (IL-1)beta nor IL-6 had an observable effect on TGF beta 1 mRNA expression. TGF beta upregulated collagen I mRNA expression. These data suggest that cytokines may influence TGF beta mRNA expression.
authors.affiliation: Medical Service, VA Medical Center, Seattle, WA 98108.
authors.forename: J
authors.initials: J
authors.lastname: Nemunaitis
authors.forename: C K
authors.initials: CK
authors.lastname: Tompkins
authors.forename: D F
authors.initials: DF
authors.lastname: Andrews
authors.forename: J W
authors.initials: JW
authors.lastname: Singer
mesh_terms: D015152:Blotting, Northern; D001853:Bone Marrow / Q000378:metabolism*; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D005786:Gene Expression Regulation; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D014170:Transformation, Genetic; D016211:Transforming Growth Factor alpha / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D007375:Interleukin-1; D015850:Interleukin-6; D012333:RNA, Messenger; D016211:Transforming Growth Factor alpha; D016212:Transforming Growth Factor beta; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D003094:Collagen