title: Adverse effects of topical prostacyclin application in microvascular surgery: an experimental study.
abstract: The efficacy of topical prostacyclin as an antithrombotic agent was tested in a model of microvascular trauma. Preliminary measurements were made to determine a suitable dosage. Twenty-seven central arteries of rabbit ears were then prepared and 32P-labelled platelets infused intraaortically. Arteriotomy (7 mm) was followed by intimectomy (5 mm). Fifteen vessels in a control group were irrigated with Ringer's lactate and 12 vessels in an experimental group were treated with prostacyclin (10 ng/ml) in normal saline. Bleeding times at the sites of arteriotomy intimectomy, in vivo accumulations of isotope-labelled platelets, amounts of red thrombotic material, and patency were recorded. Patency was lower following prostacyclin treatment (1/12 as against 5/15) but not significantly so, and there were no statistically significant differences in other parameters. Prostacyclin treatment decreased vessel wall tone, interfering with blood-flow and promoting thrombus formation.
authors.affiliation: Department of Experimental Research, Malmö General Hospital, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Salemark
authors.forename: J B
authors.initials: JB
authors.lastname: Wieslander
authors.forename: P
authors.initials: P
authors.lastname: Dougan
authors.forename: B
authors.initials: B
authors.lastname: Arnljots
mesh_terms: D000287:Administration, Topical; D000714:Anastomosis, Surgical; D000818:Animals; D001158:Arteries / Q000503:physiopathology / Q000601:surgery*; D001783:Blood Flow Velocity; D004423:Ear / Q000098:blood supply; D011464:Epoprostenol / Q000008:administration & dosage / Q000009:adverse effects*; D005260:Female; D007552:Isotonic Solutions; D008297:Male; D008866:Microsurgery; D011817:Rabbits; D000077325:Ringer's Lactate; D013927:Thrombosis / Q000139:chemically induced
chemical_list: D007552:Isotonic Solutions; D000077325:Ringer's Lactate; D011464:Epoprostenol