Characterization of a monoclonal antibody directed against the phosphotyrosine kinase p56lck, in human T cells.
A monoclonal antibody (anti-p56lck) was generated against a fusion protein containing the residues 145-509 of the human p56lck, a lymphocyte-specific membrane-associated protein tyrosine kinase. The involvement of this enzyme in T-cell transmembrane signalling seems to be an early and crucial event during T-cell receptor-mediated activation. We have produced a monoclonal antibody which recognizes p56lck in free form and when associated with CD4. It functions in western blot analysis and is capable of selectively blocking auto-phosphorylation of this kinase. This monoclonal antibody should be useful for investigating the role of p56lck in T-cell activation.
Ansotegui
I J
IJ
Department of Immunology, Karolinska Institute, Stockholm, Sweden.
Chow
S C
SC
Jeddi-Tehrani
M
M
Meloche
S
S
Pawson
A
A
Sekaly
R P
RP
Mak
T W
TW
Wigzell
H
H
D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D015704:CD4 Antigens / Q000276:immunology; D006801:Humans; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D010766:Phosphorylation / Q000187:drug effects; D011233:Precipitin Tests; D011505:Protein-Tyrosine Kinases / Q000276:immunology*; D015398:Signal Transduction / Q000187:drug effects; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D011505:Protein-Tyrosine Kinases; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck)