Analysis of workshop antibodies to null cells on a non-T/non-B lymphocyte population.
Non-T and non-B lymphocytes (null cells) were obtained by Sephadex G-10 depletion followed by treatment with mAbs to CD2 and MHC class II and complement. The enriched cells were principally CD5dim and contained greatly increased numbers of null lymphocytes. This methodology will be useful for null lymphocyte enrichment in examining cell surface molecules and functional attributes of null lymphocytes.
O'Reilly
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KL
Department of Veterinary Science, University of Wisconsin-Madison 53706.
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002417:Cattle; D002469:Cell Separation / Q000379:methods; D005434:Flow Cytometry; D008215:Lymphocytes, Null / Q000276:immunology*
D000911:Antibodies, Monoclonal