title: DNA-dependent RNA polymerase from an extremely thermophilic hydrogen-oxidizing bacterium Calderobacterium hydrogenophilum.
abstract: Extremely thermophilic bacterium Calderobacterium hydrogenophilum contains DNA-dependent RNA polymerase with unusual properties. Purified enzyme is thermoresistant (40 min at 100 degrees C) and exhibits similar subunit composition as eubacterial RNA polymerases (e.g. Escherichia coli). However, the enzyme is not susceptible to antibiotics which inhibit eubacterial RNA polymerases (rifampicin and streptolydigin). The activity of the enzyme is inhibited by actinomycin D, daunomycin and heparin.
authors.affiliation: Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
authors.forename: L
authors.initials: L
authors.lastname: Andĕra
authors.forename: K
authors.initials: K
authors.lastname: Mikulík
authors.forename: P
authors.initials: P
authors.lastname: Branny
authors.forename: M A
authors.initials: MA
authors.lastname: Puscheva
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D005051:Eubacterium / Q000201:enzymology*; D006358:Hot Temperature; D006859:Hydrogen / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010957:Plasmids; D013698:Templates, Genetic
chemical_list: D006859:Hydrogen; D012321:DNA-Directed RNA Polymerases