title: Idiopathic retinal degeneration in the dog: differential patterns of [3H]uridine incorporation and HIOMT-like immunoreactivity in surviving photoreceptors.
abstract: Photoreceptor cell pathology was investigated in an 8-yr-old mixed-breed dog which had displayed visual symptoms of 1 month duration. An electroretinogram detected no light-evoked responses. Light and electron microscopic features showed marked thinning and atrophy of the outer both the tapetal and non-tapetal retina appeared to be involved. In the non-tapetal region, a majority of the rod inner segments were missing, while scattered mitochondria-filled stubby inner segments of cones were readily identified. Inner segments of both rods and cones were observed in the tapetal region. Photoreceptor outer segments were completely absent from the affected retina, and no outer segment debris was observed between the photoreceptor layer and the retinal pigmented epithelium (RPE). Autoradiographic analysis of 3-mm retinal disks from the degenerate retina following incubation with [3H]uridine indicated that only 61% +/- 13 S.D. of the remaining nuclei of rod photoreceptors were undergoing RNA synthesis, whereas more than 99% of cone nuclei incorporated the label. Normal and degenerate retina were also analysed for localization of hydroxyindole-O-methyltransferase (HIOMT)-like immunoreactivity. While the normal retina showed immunoreactivity in both rod and cone photoreceptors with more intense immunoreactivity present in cones, the degenerate retina showed HIOMT-like immunoreactivity only in the remaining cone photoreceptors. The results of this study of idiopathic photoreceptor degeneration of the canine retina suggest that although both photoreceptor types are involved, rods are more severely affected than cones.
authors.affiliation: Department of Neurobiology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27103.
authors.forename: A F
authors.initials: AF
authors.lastname: Wiechmann
authors.forename: R L
authors.initials: RL
authors.lastname: Font
authors.forename: J G
authors.initials: JG
authors.lastname: Hollyfield
mesh_terms: D000121:Acetylserotonin O-Methyltransferase / Q000378:metabolism*; D000818:Animals; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D004596:Electroretinography; D005260:Female; D008854:Microscopy, Electron; D010786:Photoreceptor Cells / Q000648:ultrastructure; D012160:Retina / Q000648:ultrastructure; D012162:Retinal Degeneration / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D014529:Uridine / Q000378:metabolism*
chemical_list: D000121:Acetylserotonin O-Methyltransferase; D014529:Uridine