title: Cryopreservation affects endothelial and smooth muscle function of canine autogenous saphenous vein grafts.
abstract: Cryopreserved veins used as arterial grafts may be affected by both rejection and the cryopreservation process. Experiments were designed to study changes in endothelial and smooth muscle function after cryopreservation but independent of rejection. One saphenous vein from each of eight dogs was cryopreserved for subsequent use as autografts. After 3 weeks one cryopreserved and one freshly harvested autogenous saphenous vein were implanted as bilateral femoral arterial interposition grafts. Platelet deposition was studied in vivo with indium 111-labeled platelets. At 4 weeks the autografts were removed, and the functional characteristics of the grafts were studied in organ chambers; and the ability of nerve terminals to uptake transmitter was studied with 3H-norepinephrine. Neither patency rates, blood flows, nor platelet deposition were significantly different between freshly harvested and cryopreserved grafts. Uptake of 3H-norepinephrine was significantly reduced in both grafts as compared to unoperated veins. The smooth muscle of the cryopreserved and fresh grafts contracted comparably to alpha-adrenergic agonists and endothelin. In cryopreserved grafts, the maximal tensions that developed to KCl, prostaglandin F2 alpha, and endothelin were greater when the endothelium was present compared to that developed by the smooth muscle alone. Calcium ionophore A23187 caused relaxations only in rings with endothelium; these were not significantly different between graft types. However, relaxations of the smooth muscle to nitric oxide were decreased in the cryopreserved grafts.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Section of Vascular Surgery, Mayo Clinic, Rochester, MN 55905.
authors.forename: J R
authors.initials: JR
authors.lastname: Elmore
authors.forename: P
authors.initials: P
authors.lastname: Gloviczki
authors.forename: K G
authors.initials: KG
authors.lastname: Brockbank
authors.forename: V M
authors.initials: VM
authors.lastname: Miller
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000502:physiology; D015925:Cryopreservation / Q000009:adverse effects*; D004285:Dogs; D004730:Endothelium, Vascular / Q000502:physiology; D009131:Muscle, Smooth, Vascular / Q000502:physiology*; D009638:Norepinephrine / Q000378:metabolism; D012501:Saphenous Vein / Q000033:anatomy & histology / Q000502:physiology / Q000637:transplantation*; D014654:Vascular Patency / Q000502:physiology; D014661:Vasoconstriction / Q000502:physiology; D014664:Vasodilation / Q000502:physiology
chemical_list: D009638:Norepinephrine