Platelet-derived growth factor B chain homodimer enhances chemotaxis and DNA synthesis in normal osteoblast-like cells (MC3T3-E1).
We demonstrate that recombinant platelet-derived growth factor B chain homodimer (PDGF-BB) could induce both the chemotactic activity and the DNA synthesis in a normal osteoblast-like cell line (MC3T3-E1). Cell migration toward recombinant human PDGF-BB was observed with the modified Boyden chamber technique. Maximum chemotaxis was exhibited at 25 ng/ml of PDGF-BB. DNA synthesis as indicated by [3H]-thymidine incorporation was also enhanced about 4-fold at 25-100 ng/ml PDGF-BB. Our results suggested that PDGF might be one of the candidates among local coupling factors for bone remodeling.
Tsukamoto
T
T
Department of Medicine, Kobe University School of Medicine, Japan.
Matsui
T
T
Fukase
M
M
Fujita
T
T
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002633:Chemotaxis / Q000187:drug effects*; D004261:DNA Replication / Q000187:drug effects*; D007294:Inositol / Q000378:metabolism; D007295:Inositol Phosphates / Q000378:metabolism; D007700:Kinetics; D046911:Macromolecular Substances; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism
D007295:Inositol Phosphates; D046911:Macromolecular Substances; D010982:Platelet-Derived Growth Factor; D011994:Recombinant Proteins; D007294:Inositol; D002118:Calcium; D013936:Thymidine