Protective effect of vitamin E against chromosomal aberrations and mutation induced by sodium chromate in Chinese hamster V79 cells.
The effect of vitamin E on chromosomal aberrations and mutation caused by Na2CrO4 was investigated in Chinese hamster V79 cells. Pretreatment with 25 microM alpha-tocopherol succinate (vitamin E) for 24 h prior to chromate exposure (2.5-5 microM) resulted in a decrease of metal-induced chromosomal aberrations. Na2CrO4 (2.5-7.5 microM) induced mutations at the HGPRT locus, but only within a very limited concentration range. This mutagenic response could also be suppressed by pretreatment with vitamin E. These results suggest that vitamin E can protect cells from the clastogenic and mutagenic action of chromate compounds, possibly through its ability to scavenge chromium(V) and/or free radicals.
Sugiyama
M
M
Department of Medical Biochemistry, Kurume University School of Medicine, Japan.
Lin
X H
XH
Costa
M
M
D000818:Animals; D002840:Chromates / Q000494:pharmacology*; D002869:Chromosome Aberrations; D006224:Cricetinae; D004247:DNA / Q000187:drug effects; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004336:Drug Antagonism; D066298:In Vitro Techniques; D009152:Mutagenicity Tests; D009154:Mutation / Q000187:drug effects*; D017670:Sodium Compounds; D014810:Vitamin E / Q000494:pharmacology*
D002840:Chromates; D017670:Sodium Compounds; D014810:Vitamin E; C028982:sodium chromate(VI); D004247:DNA