B cell dysfunction in haemophilia in the absence and presence of HIV-1 infection.
56 haemophiliacs selected on the basis of HIV-1 antibody status, liver disease grade and mean annual dose of clotting factor concentrate used were studied. Spontaneous and stimulated IgG and IgM production in vitro were measured. HIV-1 infection was associated with increased spontaneous immunoglobulin production and an impaired response to pokeweed mitogen and Staph Aureus protein A. Implying a shift in the proportions of partially and fully activated B cells. In the absence of HIV-1 infection there was a shift to a greater proportion of partially activated B cells in patients with severe liver disease. The remainder had in vitro immunoglobulin production comparable to controls. B cell abnormalities occur early in the course of HIV-1 infection. Liver disease and not clotting factor concentrate treatment cause B cell abnormalities in the absence of HIV-1 infection in haemophilia.
Madhok
R
R
University Department of Medicine, Glasgow Royal Infirmary, Scotland.
Gracie
J A
JA
Forbes
C D
CD
Lowe
G D
GD
D000293:Adolescent; D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology*; D001779:Blood Coagulation Factors / Q000627:therapeutic use; D002469:Cell Separation; D002478:Cells, Cultured; D015483:HIV Antibodies / Q000032:analysis; D015658:HIV Infections / Q000209:etiology / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology; D006467:Hemophilia A / Q000150:complications / Q000276:immunology*; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis; D008107:Liver Diseases / Q000150:complications; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D011043:Pokeweed Mitogens; D013211:Staphylococcus aureus; D016176:T-Lymphocyte Subsets / Q000276:immunology; D065227:Transfusion Reaction
D001779:Blood Coagulation Factors; D015483:HIV Antibodies; D007136:Immunoglobulins; D011043:Pokeweed Mitogens