Transglycosylation activity of cellobiohydrolase I from Trichoderma longibrachiatum on synthetic and natural substrates.
Using 4-methylumbelliferyl (MUF) beta-D-cellobioside as a substrate, the ability of cellobiohydrolase I from Trichoderma longibrachiatum to catalyze transglycosylation has been demonstrated. At substrate concentrations greater than 2 mM, the formation of MUF-tetrasaccharide was detected using HPLC. In the course of enzymatic reaction, a concentration of the transglycosylation product passed through a maximum, since at later stages of the reaction the product was further hydrolyzed. At MUF-beta-D-cellobioside concentrations of 2-10 mM, the maximum weight content of MUF-tetrasaccharide amounted to 1-4% of the total content of saccharides. In the reaction system, containing 2.5 mM MUF-beta-D-cellobioside and 10 mM MUF-beta-D-glucoside, MUF-trisaccharide was formed as the main transglycosylation product. In hydrolysis of natural substrates (cellulose and cellotriose) in the presence of MUF-beta-D-glucoside a formation of MUF-trisaccharide was also observed.
Gusakov
A V
AV
Department of Chemistry, M.V. Lomonosov Moscow State University, U.S.S.R.
Protas
O V
OV
Chernoglazov
V M
VM
Sinitsyn
A P
AP
Kovalysheva
G V
GV
Shpanchenko
O V
OV
Ermolova
O V
OV
D002475:Cellobiose / Q000031:analogs & derivatives / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism; D043366:Cellulose 1,4-beta-Cellobiosidase; D002851:Chromatography, High Pressure Liquid; D005960:Glucosides / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D006031:Glycosylation; D006923:Hymecromone / Q000031:analogs & derivatives / Q000378:metabolism; D008956:Models, Chemical; D014242:Trichoderma / Q000201:enzymology*; D014312:Trisaccharides / Q000378:metabolism
D005960:Glucosides; D014312:Trisaccharides; D002475:Cellobiose; C014110:4-methylumbelliferyl glucoside; D006923:Hymecromone; C023872:mannotriose; C068475:4-methylumbelliferylcellobioside; D002482:Cellulose; D006026:Glycoside Hydrolases; D043366:Cellulose 1,4-beta-Cellobiosidase