Cell specific DNA alkylation in target and non-target organs of N-nitrosobis(2-oxopropyl)amine-induced carcinogenesis in hamster and rat.
Tissue-specific formation and short-term persistence of alkylated DNA bases have been studied immunocytochemically in Syrian hamsters and rats killed 3-48 h after a single s.c. or oral dose of N-nitrosobis(2-oxopropyl)amine (BOP). Antisera specific for O6-(m)ethylguanine and for 7-(m)ethylguanine were used. Strong nuclear staining, indicative of a high level of DNA alkylation, was observed at all time points in the intra- and interlobular duct cells and in the centroacinar cells of the hamster pancreas, the main target organ of BOP-induced carcinogenesis. Acinar cells were weakly stained for up to 24 h. In the liver, nuclear staining was strong in all cell types, and more pronounced in the periportal than in the central venous area. Both O6-alkylguanine and 7-alkylguanine preferentially disappeared from the centrilobular area of the liver which is in agreement with the high O6-methyltransferase activity of liver and the unusually high levels of 7-methylguanine DNA glycosylase activity in hamster tissues. Strong staining was observed throughout the experiment in the tubular cells of the renal cortex and in bronchiolar Clara and alveolar type II cells of the lung. The staining intensity of the cells of the thyroid follicles and of the columnar epithelial cells of the colon was moderate. In the rat, nuclear staining was strong in the nasal cavity (Bowman glands), the epithelium lining the thyroid follicles, the lung, liver and in the fibroblasts of the ureter intima and adventitia. The epithelial cell nuclei of the colon and ureter were moderately stained. In the pancreas, staining was weak in acinar, duct and islet cells; no acinar staining remained at 48 h. In the liver, nuclear staining was strong all over the lobule. O6-Alkylguanine was preferentially removed from the centrilobular area. The renal tubular cells were only weakly stained. From the present study we can conclude that--with the exception of hamster kidney and rat liver--high levels of DNA alkylation and stability of the alkylated products were related to a high tumor incidence.
Bax
J
J
Division of Chemical Carcinogenesis, The Netherlands Cancer Institute, Amsterdam.
Schippers-Gillissen
C
C
Woutersen
R A
RA
Scherer
E
E
D000478:Alkylation; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003106:Colon / Q000378:metabolism; D006224:Cricetinae; D004247:DNA / Q000378:metabolism*; D007150:Immunohistochemistry; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D009296:Nasal Cavity / Q000378:metabolism; D009374:Neoplasms, Experimental / Q000139:chemically induced; D009602:Nitrosamines / Q000008:administration & dosage / Q000633:toxicity*; D010179:Pancreas / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013961:Thyroid Gland / Q000378:metabolism; D014513:Ureter / Q000378:metabolism
D009602:Nitrosamines; C013622:nitrosobis(2-oxopropyl)amine; D004247:DNA