Autographa californica M nuclear polyhedrosis virus and cytochalasin D: antagonists in the regulation of protein synthesis.
During infection by the baculovirus Autographa californica multiple nuclear polyhedrosis virus (AcMNPV), synthesis of host proteins in IPLB-Sf-21 cells is inhibited. We report here that cytochalasin D (CD), a drug that specifically interacts with actin, behaved as an antagonist in the virus-mediated shutdown of host proteins actin and tubulin. In uninfected cells, CD caused an increase in actin synthesis but had no apparent effect on tubulin synthesis. In infected cells, CD similarly enhanced actin synthesis early in infection and delayed the virus shutoff of actin synthesis by 14 hr. The shutoff of tubulin synthesis was delayed by 8 hr. Addition of CD to infected cells after host protein synthesis ceased resulted in an induction of actin synthesis reversing viral inhibitory effects. Similarly, the removal of CD resulted in virus-induced inhibition of actin synthesis. Treatment of infected cells with CD caused a delay in the onset and/or shutoff of at least five viral proteins and inhibited the amplification of polyhedrin synthesis by at least 8 hr.
Talhouk
S N
SN
Department of Entomology, University of California, Berkeley 94720.
Volkman
L E
LE
D000199:Actins / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D000818:Animals; D016367:Baculoviridae / Q000502:physiology*; D002460:Cell Line; D015638:Cytochalasin D / Q000494:pharmacology*; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D007313:Insecta / Q000502:physiology; D000077183:Occlusion Body Matrix Proteins; D012333:RNA, Messenger / Q000235:genetics; D014404:Tubulin / Q000235:genetics; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics; D015678:Viral Structural Proteins; D014777:Virus Diseases / Q000378:metabolism* / Q000473:pathology*
D000199:Actins; D000077183:Occlusion Body Matrix Proteins; D012333:RNA, Messenger; D014404:Tubulin; D014764:Viral Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus; D015638:Cytochalasin D