title: The synthesis of phosphoenolpyruvate carboxylase in imbibing sorghum seeds.
abstract: When sorghum seeds were imbibed either in the light or in the dark, the presence of newly synthesized phosphoenolpyruvate carboxylase (PEPC) could be detected immunologically after approximately 6 h. In addition, both PEPC mRNA and enzyme activity were detected in extracts of dry seeds prior to inhibition. By contrast, ribulose-1,5-bisphosphate carboxylase mRNA, protein, and activity, as well as chlorophyll, were not detected even after 24 h of inhibition. These observations suggest that the nonphotosynthetic form of PEPC is synthesized during seed development and may play an important role in the germinative process.
authors.affiliation: Department of Biology, University of Waterloo, Ont., Canada.
authors.forename: E
authors.initials: E
authors.lastname: Khayat
authors.forename: E B
authors.initials: EB
authors.lastname: Dumbroff
authors.forename: B R
authors.initials: BR
authors.lastname: Glick
mesh_terms: D015152:Blotting, Northern; D002734:Chlorophyll / Q000032:analysis; D010730:Phosphoenolpyruvate Carboxylase / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D006109:Poaceae; D011233:Precipitin Tests; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D002734:Chlorophyll; D010730:Phosphoenolpyruvate Carboxylase; D012273:Ribulose-Bisphosphate Carboxylase