title: Cell surface glycosylation changes accompanying immortalization and transformation of normal human mammary epithelial cells.
abstract: Fluorescent lectin binding to cell surfaces was quantitatively analysed by flow cytometry on mortal human breast epithelial cells MCF-10M, the immortalized cell line MCF-10A derived from MCF-10M and sublines of MCF-10A transfected with the neomycin resistance gene (MCF-10Aneo), the c-Ha-ras protooncogene (MCF-10AneoN), or transfected and transformed with the c-Ha-ras activated oncogene (MCF-10AneoT). Immortal MCF-10A cells bound 10-fold more peanut agglutinin (PNA) and soy bean agglutinin (SBA) than did MCF-10M cells. Transformed MCF-10AneoT cells bound approximately ten times more PNA than did non-transformed cells transfected with protooncogene (MCF-10AneoN). Treatment of the transfectants with neuraminidase abrogated the differences in PNA-binding and reduced the differences in SBA binding. SDS-PAGE separation of PNA binding glycoproteins revealed different patterns for all MCF-10A derived sublines.
authors.affiliation: Department of Immunology, Michigan Cancer Foundation, Detroit 48201.
authors.forename: J W
authors.initials: JW
authors.lastname: Rak
authors.forename: F
authors.initials: F
authors.lastname: Basolo
authors.forename: J W
authors.initials: JW
authors.lastname: Elliott
authors.forename: J
authors.initials: J
authors.lastname: Russo
authors.forename: F R
authors.initials: FR
authors.lastname: Miller
mesh_terms: D015153:Blotting, Western; D001940:Breast; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic; D004352:Drug Resistance, Microbial / Q000235:genetics; D004848:Epithelium / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D011905:Genes, ras; D006031:Glycosylation; D006801:Humans; D037102:Lectins; D008562:Membrane Glycoproteins / Q000302:isolation & purification / Q000378:metabolism*; D009439:Neuraminidase / Q000494:pharmacology; D014162:Transfection
chemical_list: D037102:Lectins; D008562:Membrane Glycoproteins; D009439:Neuraminidase