The RGD containing site of the mouse laminin A chain is active for cell attachment, spreading, migration and neurite outgrowth.
The laminin A chain has been sequenced by cDNA cloning and was found to contain an RGD sequence. Synthetic peptides containing the RGD sequence and flanking amino acids were active in mediating cell adhesion, spreading, migration, and neurite outgrowth. Furthermore, endothelial cell attachment to a laminin substrate was inhibited by an RGD-containing synthetic peptide. Antisera against the integrin (fibronectin) receptor, and monoclonal antibody to the integrin, VLA-6, inhibited cell interaction with laminin, as well as with peptides containing an RGD sequence. These results suggest that the RGD containing site of laminin is active and interacts with the integrin family of receptors in certain cells.
Tashiro
K
K
Laboratory of Developmental Biology and Anomalies, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892.
Sephel
G C
GC
Greatorex
D
D
Sasaki
M
M
Shirashi
N
N
Martin
G R
GR
Kleinman
H K
HK
Yamada
Y
Y
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000502:physiology; D001369:Axons / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D004727:Endothelium / Q000166:cytology / Q000502:physiology; D005354:Fibrosarcoma / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D006825:Hybridomas / Q000473:pathology / Q000502:physiology; D016023:Integrins / Q000276:immunology; D007797:Laminin / Q000032:analysis / Q000737:chemistry / Q000502:physiology*; D008545:Melanoma / Q000473:pathology / Q000503:physiopathology; D051379:Mice; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000032:analysis / Q000737:chemistry / Q000502:physiology*; D011233:Precipitin Tests; D012208:Rhabdomyosarcoma / Q000473:pathology / Q000503:physiopathology
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D016023:Integrins; D007797:Laminin; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid