Detection of transmembrane linkages between immunoglobulin or complement receptors and the neutrophil's cortical microfilaments by resonance energy transfer microscopy.
By exploiting the 1/r4 (where r is the separation distance between fluorochromes) dependence of energy transfer between parallel lamellae, we have observed transmembrane energy transfer between membrane receptors and cortical microfilaments. Receptors were labeled with donor- or acceptor-conjugated Fab fragments, whereas microfilaments were labeled with acceptor- or donor-conjugated phalloidin reagents. Energy transfer was imaged by optical microscopy. We report that cell surface receptors can be constitutively unlinked, linked or inducibly linked to microfilaments.
Zhou
M J
MJ
Department of Biological Sciences, Wayne State University, Detroit, MI 48202.
Todd
R F
RF
Petty
H R
HR
D008841:Actin Cytoskeleton / Q000378:metabolism*; D000595:Amino Acid Sequence; D004735:Energy Transfer; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000378:metabolism*; D008853:Microscopy / Q000379:methods; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000378:metabolism* / Q000648:ultrastructure; D011951:Receptors, Complement / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000378:metabolism*
D007140:Immunoglobulin Fab Fragments; D011951:Receptors, Complement; D011971:Receptors, Immunologic