Cross-resistance to UV radiation of a cisplatin-resistant human cell line: overexpression of cellular factors that recognize UV-modified DNA.
A human cell line selected for cisplatin resistance (CPR) was irradiated with UV light and showed cross-resistance to UV light. Applying a modified chloramphenicol acetyltransferase assay, we observed that CPR cells acquired enhanced host cell reactivation of a transfected plasmid carrying UV damage. Gel mobility shift analysis indicated that two nuclear factors that recognize UV-modified DNA were overexpressed in CPR cells. In addition, factors that bind UV-modified DNA were independent from the factors that bind cisplatin-modified DNA. The significance of the identified binding factors, possibly DNA repair enzymes, is discussed.
Chao
C C
CC
Department of Biochemistry and Medicine, Chang Gung Medical College, Taoyuan, Taiwan, Republic of China.
Huang
S L
SL
Huang
H M
HM
Lin-Chao
S
S
D001667:Binding, Competitive; D002460:Cell Line; D002945:Cisplatin / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D004351:Drug Resistance / Q000235:genetics; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D010957:Plasmids; D014162:Transfection; D014466:Ultraviolet Rays
D004247:DNA; D002945:Cisplatin