Immunohistochemical evidence for an association of ribosomes with microfilaments in 3T3 fibroblasts.
Ribosome distribution in cultured fibroblasts was investigated immunohistochemically using antibodies which recognize the 60S ribosomal subunit. After treatment of cells with buffer containing 25mM KCl and 0.05% Nonidet-P40 immunostained material was present in punctate patterns and linear arrays consistent with some ribosomes being associated with the cytoskeleton. Treatment of the cells with 130mM KCl caused loss of both the beaded lines of immunostaining and micro-filaments. Double immunostaining showed ribosomes to be closely associated with microfilaments.
Hesketh
J E
JE
Biochemical Sciences Division, Rowett Research Institute, Bucksburn, Aberdeen, U.K.
Horne
Z
Z
Campbell
G P
GP
D008841:Actin Cytoskeleton / Q000737:chemistry / Q000276:immunology / Q000648:ultrastructure*; D000199:Actins / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D003599:Cytoskeleton / Q000737:chemistry / Q000276:immunology / Q000648:ultrastructure; D005347:Fibroblasts; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D051379:Mice; D011132:Polyribosomes / Q000737:chemistry / Q000276:immunology / Q000648:ultrastructure; D012269:Ribosomal Proteins / Q000032:analysis / Q000276:immunology; D012270:Ribosomes / Q000737:chemistry / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure*; D013053:Spectrophotometry
D000199:Actins; D000911:Antibodies, Monoclonal; D012269:Ribosomal Proteins