title: Prolongation of allograft survival in diabetic rats treated with cyclosporine by deoxyguanosine pretreatment of pancreatic islets of Langerhans.
abstract: In vitro pretreatment of islets of Langerhans with deoxyguanosine (dGuo) has been shown to be effective for the prolongation of islet allograft survival in rats. [This study evaluates the effect of pretreatment of islets with dGuo transplanted into CsA-treated recipients.] Transplantation of dGuo-treated islets from Wistar rats into diabetic hooded (PVG) rats resulted in 36% graft survival without immunosuppression (dGuo-group) and 89% islet survival after a short course of cyclosporine was used in recipients (dGuo + CsA group). In contrast, transplantation of untreated islets into rats without immunosuppression (controls) and with CsA (CsA group) immunosuppression resulted in 0 and 56% survival, respectively. The differences in graft survival between dGuo versus control group (P less than 0.001), (dGuo + CsA) versus control group (P less than 0.0001), and CsA versus control group (P less than 0.002) are statistically significant. Donor-strain skin-graft challenge failed to induce rejection of transplanted normoglycemic rats in (dGuo) and (dGuo + CsA) groups. The results indicate that a state of immunologic unresponsiveness may have been induced in the recipients of dGuo-treated islets, and further treatment with CsA synergistically prolongs islet survival in fully mismatched rats.
authors.affiliation: Department of Organ Transplantation and Pathology, Faculty of Medicine, Kuwait University, Safat.
authors.forename: I H
authors.initials: IH
authors.lastname: al-Abdullah
authors.forename: A M
authors.initials: AM
authors.lastname: Kumar
authors.forename: M S
authors.initials: MS
authors.lastname: al-Adnani
authors.forename: G M
authors.initials: GM
authors.lastname: Abouna
mesh_terms: D000818:Animals; D003524:Cyclosporins / Q000494:pharmacology*; D003849:Deoxyguanosine / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000601:surgery*; D006085:Graft Survival / Q000187:drug effects*; D007515:Islets of Langerhans / Q000187:drug effects*; D016381:Islets of Langerhans Transplantation; D008297:Male; D051381:Rats; D014184:Transplantation, Homologous
chemical_list: D003524:Cyclosporins; D003849:Deoxyguanosine