title: Development of surgical techniques for preparation of in vitro-isolated perfused porcine skin flaps for percutaneous absorption studies.
abstract: We developed a single-pedicle, axial pattern tubed skin flap that could be transferred to an in vitro perfusion apparatus. On the basis of results of prosections, angiography, contact radiography, and surviving-length studies, it was concluded that a single-pedicle, axial pattern skin flap measuring 4 cm x 12 cm incorporating the caudal superficial epigastric artery would survive to its entire length. Subsequently, a surgical (stage 1) procedure was developed for the routine preparation of single-pedicle, axial pattern tubed skin flaps. Healing after the stage-1 procedure was evaluated by visual inspection and fluorescein angiography. Stage-1 procedures were performed successfully 149 of 160 (93%) times. A second surgical (stage 2) procedure was developed for routine cannulation of the caudal superficial epigastric artery and harvest of the tubed skin flap. Stage-2 procedures were performed successfully 136 of 144 (94%) times.
authors.affiliation: Laboratory of Surgical Research, College of Veterinary Medicine, North Carolina State University, Raleigh 27606.
authors.forename: K F
authors.initials: KF
authors.lastname: Bowman
authors.forename: N A
authors.initials: NA
authors.lastname: Monteiro-Riviere
authors.forename: J E
authors.initials: JE
authors.lastname: Riviere
mesh_terms: D000818:Animals; D005260:Female; D010477:Perfusion / Q000662:veterinary; D012869:Skin Absorption; D013524:Surgical Flaps / Q000379:methods / Q000662:veterinary*; D013552:Swine