A comparative study on the xenotransplantability of human solid tumors into mice with different genetic immune deficiencies.
These experiments set out to assess the role of NK and B cells in the resistance of nude mice to human tumor xenotransplantation. The transplantability of 9 fresh and 8 cultured human tumors was compared in 2 strains of mice with different genetic immune deficiencies: athymic NCr/Sed (nu/nu) nude mice, and nude-beige-xid (N:NIH-nu-bg-xid/Sed mice). Flow cytometric studies showed both strains to be deficient in Thy. 1.2 (T) cells and unresponsive to stimulation by Concanavalin A (Con A) or direct T-cell-receptor triggering with anti-CD3. The number of B cells was similar in the 2 strains, but the response to lipopolysaccharide (LPS) was markedly reduced in the nude-beige-xid animals. The number of asialoGM1-positive cells (predominantly NK) detected by flow cytometry was also reduced in the nude-beige-xid mice. The transplantability of the human tumors was found to be equivalent in the 2 strains. Quantitative cell-transplantation assays performed for 2 of the tumor cell lines did not reveal any subtle transplantation advantage for the more broadly immune-deficient animals. No evidence could, therefore, be found to suggest that NK or B cells were major determinants of human tumor xenotransplantability in these strains of mice.
Zietman
A L
AL
Edwin L. Steele Laboratory, Department of Radiation Medicine, Massachusetts General Hospital, Boston.
Sugiyama
E
E
Ramsay
J R
JR
Silobrcic
V
V
Yeh
E T
ET
Sedlacek
R S
RS
Suit
H D
HD
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology; D005434:Flow Cytometry; D005910:Glioma / Q000276:immunology*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008819:Mice, Nude / Q000235:genetics*; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology*; D014183:Transplantation, Heterologous / Q000276:immunology
D008070:Lipopolysaccharides; D003208:Concanavalin A