title: High heterogeneity of the exopolysaccharides of Pseudomonas solanacearum strain GMI 1000 and the complete structure of the major polysaccharide.
abstract: The exopolysaccharide of Pseudomonas solanacearum, which is believed to play an important role in bacterial virulence, was considered by most authors as a homogeneous entity essentially composed of N-acetylgalactosamine. The present work demonstrates the high degree of heterogeneity of this exopolysaccharidic material, which consists of a high molecular weight acidic polysaccharide and a mainly noncarbohydrate structure as major subfractions. Rhamnose-rich polyoside and glucan fractions are also present as minor components. We report the complete structure of the acidic heteropolymer involving, in addition to N-acetylgalactosamine, equimolar ratios of two rare amino sugars, 2-N-acetyl-2-deoxy-L-galacturonic acid and 2-N-acetyl-4-N-(3-hydroxybutanoyl)-2,4,6-trideoxy-D-glucose. The structure of this acidic exopolysaccharide provides the first precise basis for the analysis of the correlation exopolysaccharide structure with pathogenicity in P. solanacearum.
authors.affiliation: Centre de Recherche de Biochimie et de Génétique Cellulaires, Toulouse, France.
authors.forename: G
authors.initials: G
authors.lastname: Orgambide
authors.forename: H
authors.initials: H
authors.lastname: Montrozier
authors.forename: P
authors.initials: P
authors.lastname: Servin
authors.forename: J
authors.initials: J
authors.lastname: Roussel
authors.forename: D
authors.initials: D
authors.lastname: Trigalet-Demery
authors.forename: A
authors.initials: A
authors.lastname: Trigalet
mesh_terms: D000116:Acetylgalactosamine / Q000737:chemistry; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D005944:Glucosamine / Q000031:analogs & derivatives / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D011549:Pseudomonas / Q000032:analysis*
chemical_list: D006603:Hexuronic Acids; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; C064841:exopolysaccharide, Pseudomonas; C068706:2-N-acetyl-4-N-(3-hydroxybutanoyl)-2,4,6-trideoxyglucose; C065803:N-acetylgalactosaminuronic acid; D000116:Acetylgalactosamine; D005944:Glucosamine