DNA binding of methyl iodide in male and female F344 rats.
The genotoxic potency of methyl iodide was investigated in a DNA binding study. Male and female F344 rats were exposed to 14C-labelled methyl iodide orally or by inhalation in a closed exposure system. DNA adducts were detected in the liver, lung, stomach and forestomach of the exposed animals. [14C]3-Methyladenine, [14C]7-methylguanine and [14C]O6-methylguanine could be identified by a combination of three different methods of hydrolysing DNA and subsequent HPLC or GC/MS analysis. The highest values of methylated guanines were determined in the stomach and forestomach of the animals following both oral and inhalative exposure. These results demonstrate a systemic genotoxic effect of methyl iodide.
Gansewendt
B
B
Institut für Arbeitsphysiologie, Universität Dortmund, FRG.
Xu
D
D
Foest
U
U
Hallier
E
E
Bolt
H M
HM
Peter
H
H
D000225:Adenine / Q000302:isolation & purification; D000280:Administration, Inhalation; D000284:Administration, Oral; D000818:Animals; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism*; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D006147:Guanine / Q000302:isolation & purification; D006847:Hydrocarbons, Iodinated / Q000008:administration & dosage / Q000378:metabolism* / Q000493:pharmacokinetics; D006868:Hydrolysis; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D011687:Purines / Q000302:isolation & purification; D051381:Rats; D011916:Rats, Inbred F344
D002250:Carbon Radioisotopes; D006847:Hydrocarbons, Iodinated; D011687:Purines; D006147:Guanine; D004247:DNA; C014055:methyl iodide; D000225:Adenine; C030985:purine