Reproducibility of a commercially available kit utilizing enzyme-linked immunosorbent assay for determination of aflatoxin in peanut butter.
The reproducibility of a commercially available kit utilising enzyme-linked immunosorbent assay (ELISA) for determination of total aflatoxins has been analysed. Peanut butter samples contaminated with aflatoxin at three concentrations (mean 5.8, 11.7 and 24.8 ng/g) were analysed on different occasions using four kits. Coefficients of variation at all concentrations ranged from 2.6% to 13.6% within replicate, 0.3% to 16.2% within plate and 6.7% to 10.2% between kits. Standard curve precision profiles showed a minimum coefficient variation (CV) of 2-4% and a working range (below 10% CV) covering almost the entire standard curve.
Ward
C M
CM
AFRC Institude of Food Research, Norwich, UK.
Morgan
M R
MR
D000348:Aflatoxins / Q000032:analysis*; D010367:Arachis / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005506:Food Contamination / Q000032:analysis*; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results
D000348:Aflatoxins; D011933:Reagent Kits, Diagnostic