Recombinant interleukin-4 enhances the chemiluminescent oxidative burst of murine peritoneal macrophages.
We evaluated the effects of murine recombinant interleukin-4 (rIL-4) on murine peritoneal macrophages. We showed a marked, dose-dependent stimulation of respiratory oxidative burst by IL-4 in peptone-elicited murine peritoneal macrophages. This effect was abolished by a neutralizing monoclonal antibody (mAb) to rIL-4 confirming that the enhanced chemiluminescence was due to IL-4. In contrast, rIL-4 depressed the respiratory oxidative burst of a transformed murine macrophage cell line, J774, in a dose-dependent mAb-reversible manner.
Tan
H P
HP
Department of Microbiology, Loma Linda University, School of Medicine, California.
Nehlsen-Cannarella
S L
SL
Garberoglio
C A
CA
Tosk
J M
JM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D002461:Cell Line, Transformed; D004305:Dose-Response Relationship, Drug; D015847:Interleukin-4 / Q000276:immunology / Q000494:pharmacology*; D008163:Luminescent Measurements; D008264:Macrophages / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010100:Oxygen / Q000378:metabolism; D010101:Oxygen Consumption / Q000502:physiology*; D010529:Peritoneal Cavity / Q000166:cytology; D011994:Recombinant Proteins / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D011994:Recombinant Proteins; D015847:Interleukin-4; D015054:Zymosan; D010100:Oxygen