Evi-1, a murine zinc finger proto-oncogene, encodes a sequence-specific DNA-binding protein.
Evi-1 was originally identified as a common site of viral integration in murine myeloid tumors. Evi-1 encodes a 120-kDa polypeptide containing 10 zinc finger motifs located in two domains 380 amino acids apart and an acidic domain located carboxy terminal to the second set of zinc fingers. These features suggest that Evi-1 is a site-specific DNA-binding protein involved in the regulation of RNA transcription. We have purified Evi-1 protein from E. coli and have employed a gel shift-polymerase chain reaction method using random oligonucleotides to identify a high-affinity binding site for Evi-1. The consensus sequence for this binding site is TGACAAGATAA. Evi-1 protein specifically protects this motif from DNase I digestion. By searching the nucleotide sequence data bases, we have found this binding site both in sequences 5' to genes in putative or known regulatory regions and within intron sequences.
Perkins
A S
AS
Mammalian Genetics Laboratory, NCI-Frederick Cancer Research and Development Center, Maryland 21702.
Fishel
R
R
Jenkins
N A
NA
Copeland
N G
NG
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D015153:Blotting, Western; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D000074008:MDS1 and EVI1 Complex Locus Protein; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods; D011519:Proto-Oncogenes; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors; D016335:Zinc Fingers / Q000235:genetics*
D004268:DNA-Binding Proteins; D000074008:MDS1 and EVI1 Complex Locus Protein; C499305:Mecom protein, mouse; D015345:Oligonucleotide Probes; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors