title: Protein phosphorylation in intact coronary endothelial cells by bradykinin.
abstract: In cultured coronary endothelial cells obtained from guinea pig hearts, bradykinin (10(-6) M) stimulated the 32Pi-incorporation into 5 substrate proteins with molecular weights corresponding to 27, 32, 60, 86 and 100 kDa. The time course of phosphorylation of the 60, 86 and 100 kDa proteins was rapid (within 30 s), but transient (max. within 1-2 min.), while the 32Pi incorporation into the 27 and 32 kDa protein was delayed but increased within 10 minutes. Ca+(+)-ionophore A 23187 (10(-5) M) and 12-O-tetradecanoylphorbol-13-acetate (TPA) (10(-5) M) both mimicked the effects of the bradykinin induced phosphorylation pattern. While A 23187 enhanced the phosphorylation of the 27, 60 and 100 kDa substrates, TPA increased the 32Pi-incorporation into the 32 and 86 kDa proteins. Furthermore the time course of protein phosphorylation elicited by A 23187 and TPA showed marked similarities to those obtained with bradykinin. Our findings are consistent with the view, that stimulation of coronary endothelial bradykinin-receptors activates both Ca+(+)-dependent protein kinases and protein kinase C.
authors.affiliation: Department of Physiology, University of Düsseldorf, FRG.
authors.forename: M
authors.initials: M
authors.lastname: Hartmann
authors.forename: M
authors.initials: M
authors.lastname: Kelm
authors.forename: J
authors.initials: J
authors.lastname: Schrader
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D001920:Bradykinin / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects / Q000502:physiology; D009569:Nitric Oxide / Q000378:metabolism; D009609:Nitrous Oxide / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D010750:Phosphoproteins / Q000096:biosynthesis; D010766:Phosphorylation; D013997:Time Factors
chemical_list: D001798:Blood Proteins; D010743:Phospholipids; D010750:Phosphoproteins; D009569:Nitric Oxide; D000001:Calcimycin; D009609:Nitrous Oxide; D001920:Bradykinin