Assay for beta-glucuronidase in species of the genus Escherichia and its applications for drinking-water analysis.
Recently, Escherichia species other than Escherichia coli have been isolated from potable water. Environmental isolates as well as clinical isolates of E. adecarboxylata, E. blattae, E. fergusonii, E. hermannii, and E. vulneris were assayed for the enzyme beta-glucuronidase by using EC MUG medium and the Colilert system. None of the isolates were positive for the enzyme by either method.
Rice
E W
EW
U.S. Environmental Protection Agency, Cincinnati, Ohio 45268.
Allen
M J
MJ
Brenner
D J
DJ
Edberg
S C
SC
D004925:Escherichia / Q000201:enzymology* / Q000302:isolation & purification; D005456:Fluorescent Dyes; D005966:Glucuronidase / Q000032:analysis*; D006923:Hymecromone / Q000031:analogs & derivatives; D014871:Water Microbiology; D014881:Water Supply / Q000032:analysis*
D005456:Fluorescent Dyes; D006923:Hymecromone; C034888:4-methylumbelliferyl glucuronide; D005966:Glucuronidase