Impairment by ethanol of prostaglandin production in rat salivary glands.
Sublingual salivary acini and submandibular tissue were incubated in DMEM medium in the presence of various concentrations (0-5%) of ethanol and the content of the three major prostaglandins, PGE2, PGF2 alpha and 6-keto-PGF1 alpha, were determined by radioimmunoassay. In In the sublingual gland, ethanol caused a decrease in PGE2 and PGF2 alpha levels, but had no effect on 6-keto-PGF1 alpha, while all three prostaglandins were affected in the submandibular gland. At 2.5% ethanol, the production of PGF2 alpha and PGE2 in sublingual gland decreased by 10% and reached maximum inhibition at 5% ethanol, at which concentration there was a 20 and 30% decrease in their levels. In submandibular gland, 2.5% ethanol caused a 20% decrease in PGE2, 30% in PGF2 alpha and 50% in 6-keto-PGF1 alpha; 40% inhibition in PGE2, 57% in 6-keto-PGF1 alpha and 65% in PGF2 alpha occurred in the presence of 5% ethanol. These findings suggest that alcohol impairs the function of salivary glands by inhibiting prostaglandin production.
Wu-Wang
C Y
CY
Research Center, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark 07103.
Wang
S L
SL
Lim
C
C
Slomiany
A
A
Slomiany
B L
BL
D015121:6-Ketoprostaglandin F1 alpha / Q000032:analysis / Q000037:antagonists & inhibitors*; D000818:Animals; D046508:Culture Techniques; D015237:Dinoprost / Q000032:analysis / Q000037:antagonists & inhibitors*; D015232:Dinoprostone / Q000032:analysis / Q000037:antagonists & inhibitors*; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012469:Salivary Glands / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D013361:Sublingual Gland / Q000737:chemistry / Q000187:drug effects; D013363:Submandibular Gland / Q000737:chemistry / Q000187:drug effects; D013997:Time Factors
D000431:Ethanol; D015121:6-Ketoprostaglandin F1 alpha; D015237:Dinoprost; D015232:Dinoprostone