title: Mechanistic stoichiometry of mitochondrial oxidative phosphorylation.
abstract: P/O ratios of rat liver mitochondria were measured with particular attention to systematic errors. Corrections for energy loss during oxidative phosphorylation were made by measurement of respiration as a function of mitochondrial membrane potential. The corrected values were close to 1, 0.5, and 1 at the three coupling sites, respectively. These values are consistent with recent measurements of mitochondrial proton transport.
authors.affiliation: Section of Biochemistry, Molecular, and Cell Biology, Cornell University, Ithaca, New York 14853.
authors.forename: P C
authors.initials: PC
authors.lastname: Hinkle
authors.forename: M A
authors.initials: MA
authors.lastname: Kumar
authors.forename: A
authors.initials: A
authors.lastname: Resetar
authors.forename: D L
authors.initials: DL
authors.lastname: Harris
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000818:Animals; D004579:Electron Transport; D008314:Malonates / Q000494:pharmacology; D008564:Membrane Potentials; D008930:Mitochondria, Liver / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats
chemical_list: D008314:Malonates; D010710:Phosphates; D009243:NAD; D000244:Adenosine Diphosphate; C030290:malonic acid; D010100:Oxygen