title: Structural determination and immunochemical characterization of the type V group B Streptococcus capsular polysaccharide.
abstract: The type V capsular polysaccharide of group B Streptococcus has been isolated and purified, and its repeating unit structure determined. The native type V polysaccharide contains D-glucose, D-galactose, 2-acetamido-2-deoxy-D-glucose, and sialic acid in a molar ratio of 3:2:1:1. Methylation analysis and 1H NMR and 13C NMR analysis of the native type V polysaccharide and of its specifically degraded products permitted the determination of the repeating unit structure of the type V polysaccharide: [formula: see text] The type V polysaccharide has certain structural features in common with other group B streptococcal capsular polysaccharides but is antigenically distinct: no immunologic cross-reactivity was observed between type V and types Ia, Ib, II, III, or IV polysaccharides. Studies of antibody binding to the partially degraded forms of the type V polysaccharide indicated that the native epitope is complex, involving most if not all of the sugar residues of the repeating unit.
authors.affiliation: Channing Laboratory, Brigham and Women's Hospital, Boston, Massachusetts 02115.
authors.forename: M R
authors.initials: MR
authors.lastname: Wessels
authors.forename: J L
authors.initials: JL
authors.lastname: DiFabio
authors.forename: V J
authors.initials: VJ
authors.lastname: Benedì
authors.forename: D L
authors.initials: DL
authors.lastname: Kasper
authors.forename: F
authors.initials: F
authors.lastname: Michon
authors.forename: J R
authors.initials: JR
authors.lastname: Brisson
authors.forename: J
authors.initials: J
authors.lastname: Jelínková
authors.forename: H J
authors.initials: HJ
authors.lastname: Jennings
mesh_terms: D016667:Bacterial Capsules; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D013292:Streptococcus agalactiae
chemical_list: D007202:Indicators and Reagents; D011135:Polysaccharides, Bacterial; C068312:capsular polysaccharide, streptoccal group B type V