title: 1H NMR identification of a beta-sheet structure and description of folding topology in putidaredoxin.
abstract: Putidaredoxin (Pdx), a 106-residue globular protein consisting of a single polypeptide chain and a [2Fe-2S] cluster, is the physiological reductant of P-450cam, which in turn catalyzes the monohydroxylation of camphor by molecular oxygen. No crystal structure has been obtained for Pdx or for any closely homologous protein. The application of two-dimensional 1H NMR methods to the problem of structure determination in Pdx is reported. A beta-sheet consisting of five short strands and one beta-turn has been identified from distinctive nuclear Overhauser effect patterns. All of the backbone resonances and a majority of the side-chain resonances corresponding to protons in the beta-sheet have been assigned sequence specifically. The sheet contains one parallel and three antiparallel strand orientations. Hydrophobic side chains in the beta-sheet face primarily toward the protein interior, except for a group of three valine side chains that are apparently solvent exposed. The potential significance of this "hydrophobic patch" in terms of biological activity is discussed. The folding topology, as determined by the constraints of the beta-sheet, is compared with that of other [2Fe-2S] proteins for which folding topologies are known.
authors.affiliation: Department of Chemistry, Brandeis University, Waltham, Massachusetts 02254.
authors.forename: T C
authors.initials: TC
authors.lastname: Pochapsky
authors.forename: X M
authors.initials: XM
authors.lastname: Ye
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry; D005288:Ferredoxins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D011487:Protein Conformation; D012689:Sequence Homology, Nucleic Acid
chemical_list: D001426:Bacterial Proteins; D005288:Ferredoxins; C100137:putidaredoxin; D006859:Hydrogen