Glucagon-stimulated lipolysis of primary cultured broiler adipocytes.
A procedure for maintaining broiler adipocytes in culture was established and used to evaluate the effect of selected culture ingredients on glucagon-stimulated lipolysis. Adipocytes were isolated by collagenase and trypsin digestion of abdominal adipose tissue from 40- to 70-day-old broilers. Freshly isolated adipocytes did not exhibit glucagon-stimulated lipolysis. However, after 24 h in culture, lipolysis was stimulated maximally at doses of glucagon from 5 to 100 ng/mL with 50% stimulation occurring at .7 +/- .4 ng/mL. This responsiveness was maintained for an additional 24 h in culture. Inclusion of 2 or 5% chicken serum in the medium reduced (P less than or equal to .05) the responsiveness of the cells to glucagon. A 30% reduction (P less than or equal to .001) in responsiveness occurred when bovine serum albumin was removed from the medium. The results indicate that broiler adipocytes can be maintained in culture and that certain culture ingredients alter the responsiveness of the cells to glucagon.
Oscar
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Division of Animal and Veterinary Sciences, West Virginia University, Morgantown 26506-6108.
D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D005934:Glucagon / Q000494:pharmacology*; D008066:Lipolysis / Q000187:drug effects*
D003470:Culture Media; D005934:Glucagon