title: Multicentre comparison of the Novo Biolabs immunometric CK-MB assay with four established methods.
abstract: The analytical performance of a new immunometric CK-MB assay from Novo Biolabs was evaluated in three hospitals. Comparisons were made with another immunometric assay, an immune inhibition/precipitation technique, an immune inhibition method, and a column separation method. The Novo assay exhibited good precision (CV 3-7%) at all concentration levels tested (6-150 micrograms/L) and was found to be linear over the range 0-200 micrograms/L. Comparison of the Novo kit with the other methods using patients' sera showed acceptable correlation between the methods over a wide concentration range (r = 0.96-0.99). We conclude that the assay is a useful adjunct to the specific measurement of CK-MB, particularly in routine laboratories with a high throughput of such determinations.
authors.affiliation: Department of Clinical Chemistry, Academic Hospital Rotterdam-Dijkzigt, The Netherlands.
authors.forename: R W
authors.initials: RW
authors.lastname: Wulkan
authors.forename: J
authors.initials: J
authors.lastname: van Loon
authors.forename: A
authors.initials: A
authors.lastname: Sturk
authors.forename: G J
authors.initials: GJ
authors.lastname: Boerma
authors.forename: M C
authors.initials: MC
authors.lastname: Verdegaal
authors.forename: B
authors.initials: B
authors.lastname: van den Berg
authors.forename: J H
authors.initials: JH
authors.lastname: Souverijn
authors.forename: G T
authors.initials: GT
authors.lastname: Sanders
mesh_terms: D003402:Creatine Kinase; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007231:Infant, Newborn; D007527:Isoenzymes; D015337:Multicenter Studies as Topic; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results
chemical_list: D007527:Isoenzymes; D011933:Reagent Kits, Diagnostic; D003402:Creatine Kinase