Induction of protective immunity to Friend murine leukemia virus in genetic nonresponders to virus envelope protein.
(B10.A x A/WySn)F1, H-2a/a, mice are genetic nonresponders to the envelope protein of Friend murine leukemia helper virus (F-MuLV) when immunized with a recombinant vaccinia virus expressing F-MuLV env gene. In contrast these mice can be protectively immunized against leukemogenic Friend virus complex using formalin-fixed F-MuLV virions in CFA. To determine which viral proteins were responsible for this immune protection, virion proteins prepared by SDS-PAGE and electroelution were used to immunize mice. Purified gp70 envelope protein in CFA was capable of inducing strong immune protection against the challenge with Friend virus complex in H-2a/a mice. Immunologic studies demonstrated that immunized mice developed a virus-specific T cell proliferative response and showed IgM to IgG Ig class switching of virus-neutralizing antibodies. These results indicated that genetically controlled immune nonresponsiveness to F-MuLV envelope Ag in H-2a/a mice could be overcome using denatured viral envelope protein together with a strong adjuvant.
Ishihara
C
C
United States Department of Health and Human Services, National Institutes of Health, National Institute of Allergy and Infectious Diseases, Hamilton, MT 59840.
Miyazawa
M
M
Nishio
J
J
Chesebro
B
B
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D005260:Female; D005622:Friend murine leukemia virus / Q000276:immunology*; D006183:H-2 Antigens / Q000235:genetics*; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis; D008297:Male; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology; D014616:Vaccinia virus / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D006183:H-2 Antigens; D007074:Immunoglobulin G; D014759:Viral Envelope Proteins