Preparation of europium-labeled derivatives of cortisol for time-resolved fluoroimmunoassays.
Cortisol 3-(O-carboxymethyl)oxime, 6- and 21-hemisuccinoxycortisol, and cortisol 7-carboxyethyl thio-ether were synthesized. These carboxyl derivatives were labeled using a described general labeling method with a europium chelate. The labeled steroids were tested in a competitive time-resolved fluoroimmunoassay using antibodies raised against cortisol. Only the site-homologous antigen-antibody pairs underwent immunoreaction and gave satisfactory calibration curves.
Mikola
H
H
Wallac Chemical Laboratories, Turku, Finland.
Miettinen
P
P
D005063:Europium / Q000737:chemistry*; D015200:Fluoroimmunoassay / Q000379:methods; D006854:Hydrocortisone / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D015394:Molecular Structure; D013997:Time Factors
D005063:Europium; D006854:Hydrocortisone