title: Sensitivity of an antigen detection enzyme immunoassay for diagnosis of Trypanosoma congolense infections in goats and cattle.
abstract: The sensitivity of a monoclonal antibody-based antigen-detection enzyme immunoassay (antigen-ELISA) for the diagnosis of Trypanosoma congolense was evaluated using sera from experimentally infected goats and cattle. Ten goats (Galla x East African Masai) and 7 steers (Bos indicus) were infected with different clones of T. congolense and left to run a chronic course for 46 and 24 mo, respectively. During this period, monthly blood samples were collected and analyzed for the presence of trypanosomes and antigens in peripheral blood. Of 383 caprine blood samples, 361 (94.3%) were positive for circulating antigens whereas only 42 (10.9%) had demonstrable trypanosomes as revealed by the microhematocrit centrifugation technique. In cattle, 570 (82.5%) of 691 blood samples were antigen-ELISA positive compared to 136 (19.7%) samples with detectable trypanosomes. In an analysis of serum samples from goats in an area known to be endemic for trypanosomiasis, 106 (80.9%) of 131 were positive for T. congolense antigens whereas none of the corresponding blood samples had detectable trypanosomes. Control sera from 24 goats in a trypanosomiasis-free region were all antigen-ELISA negative. Hence, the antigen-ELISA was at least 4 times more sensitive than the microhematocrit centrifugation technique in monitoring T. congolense infections in goats and cattle.
authors.affiliation: International Laboratory for Research on Animal Diseases, Nairobi, Kenya.
authors.forename: R A
authors.initials: RA
authors.lastname: Masake
authors.forename: V M
authors.initials: VM
authors.lastname: Nantulya
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000097:blood*; D002417:Cattle; D002498:Centrifugation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015511:Goat Diseases / Q000175:diagnosis*; D006041:Goats; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011237:Predictive Value of Tests; D014348:Trypanosoma congolense / Q000276:immunology*; D014353:Trypanosomiasis, African / Q000175:diagnosis / Q000662:veterinary*; D014354:Trypanosomiasis, Bovine / Q000175:diagnosis*
chemical_list: D000953:Antigens, Protozoan