Effect of progesterone on renin secretion in endometrial stromal, chorionic trophoblast, and mesenchymal monolayer cultures.
Renin messenger ribonucleic acid expression has been shown in decidual tissue and endometrium, but weakly in chorion laeve, suggesting decidua as a primary source of uteroplacental renin. We proposed to confirm active renin secretion by endometrial stromal cells and examined the effect of progesterone-induced decidualization on secretion. Separate monolayer cultures of endometrial stroma, trophoblast, and mesenchymal cells were established and maintained for 10 to 15 days. Active renin concentration was measured with radioimmunoassay for angiotensin I generation and expressed as picograms per milliliter of angiotensin I generation per 10(5) cells. Active renin concentration was high in control secretory endometrial stromal cells (513 pg/ml) compared with trophoblast (none) and mesenchymal cell cultures (74 pg/ml). Marked decrease in active renin secretion occurred in control endometrial stromal cells (days 13 to 15, 86 pg/ml), whereas progesterone-induced decidualization sustained and increased secretion (days 13 to 15, 1017 pg/ml). This renin activity was quantitatively inhibited in culture fluid assays by specific human renal renin antibody in serial dilutions. Renin activity measurements before and after trypsin activation showed the majority of renin (91.15%) in the inactive form and a smaller fraction (8.85%) in the active form. Immunohistochemistry with the use of specific human renal renin antibody confirmed the presence of renin and its stimulation by progesterone in endometrial stromal cells. Progesterone had minimal effect on mesenchymal cells. These data confirmed endometrial stromal cells as a significant source of active renin and showed that progesterone induced a marked increase in this production.
Shah
D M
DM
Department of Obstetrics and Gynecology, University of Texas Health Science Center, San Antonio 78284-7836.
Higuchi
K
K
Inagami
T
T
Osteen
K G
KG
D002823:Chorion / Q000201:enzymology*; D046508:Culture Techniques; D004717:Endometrium / Q000201:enzymology*; D004789:Enzyme Activation; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D011374:Progesterone / Q000494:pharmacology*; D012083:Renin / Q000378:metabolism*; D014327:Trophoblasts / Q000201:enzymology*; D014357:Trypsin / Q000378:metabolism
D011374:Progesterone; D014357:Trypsin; D012083:Renin