Identification of toxigenic Clostridium botulinum type E by enzyme immunoassay.
A sensitive double-sandwich enzyme immunoassay for identification of toxigenic Clostridium botulinum type E was developed with a type-E-specific antitoxin. The antitoxin was produced in a rabbit, following inoculation of minute quantities of type-E toxoid. The toxoid was prepared from a commercially available toxin by using a detoxification method. Cross-reactivity of the antitoxin with C. botulinum types A and B was avoided by absorption of the serum with alum-precipitated toxoids A and B. The immunoassay proved to be specific for C. botulinum type E because it did not react with culture filtrates of various Clostridium species, including nontoxigenenic "E-like" organisms and Clostridium sporogenes.
Dezfulian
M
M
Department of Medical Laboratory Sciences, Florida International University, Miami 33199.
Bartlett
J G
JG
D000818:Animals; D001905:Botulinum Toxins / Q000096:biosynthesis* / Q000276:immunology; D003014:Clostridium botulinum / Q000302:isolation & purification* / Q000378:metabolism; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera / Q000276:immunology; D007114:Immunization; D007117:Immunization, Secondary; D008297:Male; D011817:Rabbits; D014121:Toxoids / Q000276:immunology
D007106:Immune Sera; D014121:Toxoids; D001905:Botulinum Toxins