title: Copy number and chromosomal location of Saccharomyces cerevisiae tRNA(Trp)-encoding genes.
abstract: Yeast tRNA(Trp)-encoding genes have been identified by Southern-blot analysis of chromosomal DNA. Seven copies of this gene are detected in blots of both restriction endonuclease digests and intact chromosomal DNA. Electrophoretic mapping of tDNA(Trp) indicates one copy is located on each of chromosomes X, XI, XIII, and XVI. The remaining three copies are localized to chromosomes VII and/or XV. Three different yeast strains gave identical results indicating this multi-gene family is relatively stable.
authors.affiliation: Department of Molecular Biology, University of Wyoming, Laramie 82071.
authors.forename: K
authors.initials: K
authors.lastname: Yesland
authors.forename: D
authors.initials: D
authors.lastname: Kim
authors.forename: J D
authors.initials: JD
authors.lastname: Johnson
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012364:RNA, Transfer, Trp / Q000235:genetics*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D015345:Oligonucleotide Probes; D012364:RNA, Transfer, Trp