Fc receptors do not mediate African swine fever virus replication in macrophages.
Titration experiments in swine macrophages have shown that African swine fever virus infectivity was not enhanced in the presence of antiviral antibodies. The early viral protein synthesis and the viral DNA replication in swine macrophages infected with virus-antibody complexes were inhibited in the presence of high doses of uv-inactivated virus, which saturated specific virus receptors, but not when Fc receptors were saturated with antibodies. These results indicate that African swine fever virus does not infect swine macrophages through Fc receptors and that the normal entry pathway through virus receptors is not bypassed by the virus-antibody complexes.
Alcamí
A
A
Centro de Biología Molecular (CSIC-UAM), Facultad de Ciencias, Universidad Autónoma, Madrid, Spain.
Viñuela
E
E
D000358:African Swine Fever Virus / Q000254:growth & development / Q000276:immunology* / Q000528:radiation effects; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D008264:Macrophages / Q000382:microbiology*; D011961:Receptors, Fc / Q000276:immunology* / Q000528:radiation effects; D014466:Ultraviolet Rays; D014709:Vero Cells / Q000382:microbiology; D014775:Virus Activation / Q000528:radiation effects; D014779:Virus Replication / Q000528:radiation effects
D000914:Antibodies, Viral; D004279:DNA, Viral; D011961:Receptors, Fc