Immunohistochemical localization of porcine diazepam-binding inhibitor (DBI) to rat endocrine pancreas.
The occurrence of diazepam-binding inhibitor (DBI), isolated and characterized from porcine upper intestine, was examined in the pancreas of Sprague-Dawley albino rats using indirect immunofluorescence. The polypeptide was found in the endocrine Langerhans islets and, utilizing double-labelling controls, it was shown to be present within the peripherally located glucagon-containing cells. Regulation of islet hormone production may therefore be under DBI control.
Johansson
O
O
Department of Histology and Neurobiology, Karolinska Institutet, Stockholm, Sweden.
Hilliges
M
M
Ostenson
C G
CG
Sandberg
E
E
Efendic
S
S
Mutt
V
V
D000818:Animals; D026261:Diazepam Binding Inhibitor; D005455:Fluorescent Antibody Technique; D005934:Glucagon / Q000032:analysis; D007515:Islets of Langerhans / Q000737:chemistry* / Q000166:cytology; D009479:Neuropeptides / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013552:Swine
D026261:Diazepam Binding Inhibitor; D009479:Neuropeptides; D005934:Glucagon