[The yields of the processes of DNA radiation-chemical destruction in the chromatin].
The methods of gel electrophoresis and spectrophotometry were used to study DNA destruction in the chromatin (a nucleosome set of 1 to 8 units) irradiated with 60Co-gamma quanta in water solutions at doses ranging from 1 to 40 kGy, depending on the chromatin concentration, the nature of gas that saturated the solution (Ar, N2O), and the protein/DNA ratio in the chromatin samples. In conditions of a complete involvement of water radiolysis products, OH and eaq, in the reaction with the chromatin (the chromatin concentrations in the solution exceeding 0.04 weight % when calculated per DNA) determined were G ("DNA-protein") cross-links = 6.10(-5), the G (chromophoric group degradation) = 0.4, and G (double-strand breaks) less than 10(-5), as well as G of the process of free DNA release into the solution. The latter depend upon the protein/DNA ratio in the sample and increased with increasing protein content of the chromatin with the protein/DNA ratio being greater than 1.1/1.
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D000818:Animals; D002843:Chromatin / Q000737:chemistry / Q000528:radiation effects*; D003037:Cobalt Radioisotopes; D004247:DNA / Q000032:analysis / Q000528:radiation effects*; D004249:DNA Damage / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004587:Electrophoresis, Agar Gel; D066298:In Vitro Techniques; D051379:Mice; D008970:Molecular Weight; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
D002843:Chromatin; D003037:Cobalt Radioisotopes; D012996:Solutions; D004247:DNA