title: Preliminary X-ray crystallographic analysis of a modified basic fibroblast growth factor.
abstract: Human basic fibroblast growth factor (hbFGF) has been modified, with Ala3 and Ser5 substituted by glutamic acid, and the purified recombinant protein has been crystallized. The crystals are triclinic (space group P1) with unit cell parameters a = 31.0 A, b = 33.6 A, c = 34.7 A, alpha = 88 degrees, beta = 85 degrees, gamma = 76 degrees, and they diffract to at least 2 A.
authors.affiliation: Department of Chemistry, Princeton University, NJ 08544.
authors.forename: D M
authors.initials: DM
authors.lastname: Bollag
authors.forename: A P
authors.initials: AP
authors.lastname: Seddon
authors.forename: P
authors.initials: P
authors.lastname: Böhlen
authors.forename: Y
authors.initials: Y
authors.lastname: Gluzman
authors.forename: C E
authors.initials: CE
authors.lastname: Schutt
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry* / Q000235:genetics; D005813:Genes, Synthetic; D006801:Humans; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D014961:X-Ray Diffraction
chemical_list: D000596:Amino Acids; D011994:Recombinant Proteins; D016222:Fibroblast Growth Factor 2