Hypersensitive site 4 of the human beta globin locus control region.
The Locus Control Region (LCR) of the human beta globin gene domain is defined by four erythroid-specific DNasel hypersensitive sites (HSS) located upstream of this multigene cluster. The LCR confers copy number dependent high levels of erythroid specific expression to a linked transgene, independent of the site of integration. To assess the role of the individual hypersensitive sites of the LCR, we have localized HSS4 to a 280bp fragment that is functional both in murine erythroleukaemia (MEL) cells and in transgenic mice. This fragment coincides with the major area of hypersensitivity 'in vivo' and contains a number of DNasel footprints. Bandshift analysis shows that these footprints correspond to binding sites for the erythroid specific proteins GATA1 and NF-E2 and a number of ubiquitous proteins, including jun/fos, Sp1 and TEF2.
Pruzina
S
S
National Institute for Medical Research, Mill Hill, London, UK.
Hanscombe
O
O
Whyatt
D
D
Grosveld
F
F
Philipsen
S
S
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D015139:Blotting, Southern; D002874:Chromosome Mapping; D004247:DNA / Q000032:analysis; D016172:DNA Fingerprinting; D003850:Deoxyribonuclease I / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D005914:Globins / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D012045:Regulatory Sequences, Nucleic Acid
D005914:Globins; D004247:DNA; D003850:Deoxyribonuclease I