Localization of placental lactogen-I in trophoblast giant cells of the mouse placenta.
The purpose of this investigation was to identify the cellular origin of placental lactogen-I (PL-I) expression in the mouse placenta and to cytologically define the transition from PL-I to PL-II expression during gestation. PL-I mRNA expression was assessed by in situ hybridization, and expression of PL-I and PL-II protein was determined by immunocytochemical analysis. PL-I mRNA and protein were localized to trophoblast giant cells. Trophoblast giant cells ceased producing PL-I at midgestation and began expressing PL-II. PL-I immunoreactivity was present in trophoblast giant cells on Days 9 and 10 of gestation but was not detectable in trophoblast giant cells on Day 11 of gestation. Immunoreactive PL-II-producing giant cells were detected first on Day 10 of gestation, continuing on Day 11 of gestation. Expression of PL-I and PL-II signals a significant functional transition in trophoblast giant cells of the developing mouse placenta.
Faria
T N
TN
Department of Physiology, University of Kansas Medical Center, Kansas City 66103.
Ogren
L
L
Talamantes
F
F
Linzer
D I
DI
Soares
M J
MJ
D000818:Animals; D005260:Female; D015726:Giant Cells / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D009693:Nucleic Acid Hybridization; D010920:Placenta / Q000378:metabolism*; D010928:Placental Lactogen / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism
D012333:RNA, Messenger; C062303:placental lactogen II; D010928:Placental Lactogen