Molecular cloning and characterization of the gene encoding the adenine methyltransferase M.CviRI from Chlorella virus XZ-6E.
The gene encoding the DNA methyltransferase M.CviRI from Chlorella virus XZ-6E was cloned and expressed in Escherichia coli. M.CviRI methylates adenine in TGCA sequences. DNA containing the M.CviRI gene was sequenced and a single open reading frame of 1137 bp was identified which could code for a polypeptide of 379 amino acids with a predicted molecular weight of 42,814. Comparison of the M.CviRI predicted amino acid sequence with another Chlorella virus and 14 bacterial adenine methyltransferases revealed extensive similarity to the other Chlorella virus enzyme.
Stefan
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Department of Plant Pathology, University of Nebraska, Lincoln 68583-0722.
Xia
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Van Etten
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D000595:Amino Acid Sequence; D001483:Base Sequence; D002708:Chlorella; D003001:Cloning, Molecular; D015254:DNA Modification Methylases / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D016415:Sequence Alignment; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000235:genetics*; D014780:Viruses / Q000201:enzymology*
D015254:DNA Modification Methylases; C068228:DNA modification methylase CviRI; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)