A comparison of swim-down and swim-up methods for the extraction of high motility sperm.
High quality motile sperm are essential for the success of the in vitro fertilization-embryo transfer program and related procedures, for the performance of sperm antibody assays, and other in vitro tests of sperm function. Two methods for the extraction of high motility sperm, swim-down and swim-up, were compared on 30 ejaculates, and sperm motility was assessed at intervals up to 18 hours. The swim-down method was simpler, shorter, and provided a significantly better recovery of sperm with sustained high motility than did the swim-up method.
Ing
R M
RM
Department of Obstetrics and Gynecology, Flinders Medical Centre, Bedford Park, South Australia.
Li
D Q
DQ
Harding
A M
AM
Jones
W R
WR
D000704:Analysis of Variance; D006801:Humans; D008297:Male; D013048:Specimen Handling / Q000379:methods*; D013081:Sperm Motility; D013094:Spermatozoa; D013997:Time Factors