Oncogene rearrangements in chronic B-cell leukemia.
Forty-four B-chronic lymphocytic leukemias (CLL) were studied by Southern blot analysis using probes for the Ig genes and bcl-1, bcl-2 (major, minor and 5' breakpoint region), bcl-3, c-myc, and retinoblastoma (Rb) loci. Eight cases had three or more rearranged JH bands, indicating oligoclonality, clonal evolution, or chromosomal translocation. One case had a rearrangement of the bcl-1 locus and three of the bcl-2 locus. In the first case, comigration of the rearranged bcl-1 and JH sequences indicated a t(11;14)(q13;q32) translocation, which, in contrast to previously described cases, seems to be completely reciprocal. One case with a bcl-2 rearrangement showed comigration of the bcl-2 major breakpoint region and a rearranged JH band. This indicates a t(14;18) (q32;q21). The two other cases showed rearrangements of the bcl-2 5' breakpoint region without apparent comigration. No rearrangements were detected of c-myc and bcl-3, located at chromosome 19, nor was a deletion of Rb found. All but three cases had CD5 expression. The exceptions included the t(11;14) and the t(14;18) cases. Our results confirm recent data on rearrangements at the 5' site of bcl-2 in CLL. Additionally, they corroborate the presumption that CD5-negative chronic B-cell leukemias should be considered apart from classical CLL.
Raghoebier
S
S
Department of Pathology, University of Leiden, The Netherlands.
van Krieken
J H
JH
Kluin-Nelemans
J C
JC
Gillis
A
A
van Ommen
G J
GJ
Ginsberg
A M
AM
Raffeld
M
M
Kluin
P M
PM
D015703:Antigens, CD / Q000235:genetics; D015139:Blotting, Southern; D002874:Chromosome Mapping; D004273:DNA, Neoplasm / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D016161:Genes, Retinoblastoma; D005838:Genotype; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood / Q000235:genetics* / Q000276:immunology; D009857:Oncogenes; D010641:Phenotype; D015183:Restriction Mapping
D015703:Antigens, CD; D004273:DNA, Neoplasm