title: Transport in isolated rat hepatocytes of the phospho-oligosaccharide that mimics insulin action. Effects of adrenalectomy and glucocorticoid treatment.
abstract: The addition to intact cells of an inositol phospho-oligosaccharide (POS), which is the polar head-group of an insulin-sensitive glycosylphosphatidylinositol, mimics and may mediate some of the biological effects of this hormone. Here we report the existence of a POS transport system in hepatocytes. This POS transport system is specific and time- and dose-dependent. Insulin-resistance caused by dexamethasone administration to rats was accompanied by a decrease in the hepatocyte POS transport system. In contrast, bilateral adrenalectomy provoked a significant increase in the transport of POS. Both the temporal uptake of POS and the regulation of this process by conditions known to modify the sensitivity to insulin suggest that this novel transport system might be involved in the insulin signalling mechanism.
authors.affiliation: Instituto de Investigaciones Biomédicas, C.S.I.C., Madrid, Spain.
authors.forename: J F
authors.initials: JF
authors.lastname: Alvarez
authors.forename: J A
authors.initials: JA
authors.lastname: Sánchez-Arias
authors.forename: A
authors.initials: A
authors.lastname: Guadaño
authors.forename: F
authors.initials: F
authors.lastname: Estévez
authors.forename: I
authors.initials: I
authors.lastname: Varela
authors.forename: J E
authors.initials: JE
authors.lastname: Felíu
authors.forename: J M
authors.initials: JM
authors.lastname: Mato
mesh_terms: D000315:Adrenalectomy; D000818:Animals; D001692:Biological Transport; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D007295:Inositol Phosphates / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008112:Liver Glycogen / Q000096:biosynthesis; D008297:Male; D009844:Oligosaccharides / Q000378:metabolism* / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism; D011134:Polysaccharides; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
chemical_list: D007295:Inositol Phosphates; D007328:Insulin; D008112:Liver Glycogen; D009844:Oligosaccharides; D010716:Phosphatidylinositols; D011134:Polysaccharides; C028039:inositol phosphate glycan; D003907:Dexamethasone; D005947:Glucose