Geometry of adult rat sensory neurons in culture; its modulation by laminin.
Geometrical analysis of highly-purified and low-density adult rat dorsal root ganglion (DRG) neuron culture proved that laminin enhanced the total neurite length and the maximal extension of neurites, but did not significantly increase the neurite branching nor soma size particularly after 4 days of culture. Survival rates of adult DRG neurons were not affected by laminin. These results suggest that laminin is a potent modulator of neurite geometry in adult rat DRG neurons, which promotes neurite elongation rather than neurite branching.
Itoh
T
T
Fourth Department of Internal Medicine, Aichi Medical University, Nagakute, Japan.
Sobue
G
G
Yasuda
T
T
Mitsuma
T
T
Takahashi
A
A
Kimata
K
K
D000818:Animals; D001369:Axons / Q000648:ultrastructure; D002470:Cell Survival; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000166:cytology / Q000648:ultrastructure; D007797:Laminin / Q000494:pharmacology*; D009475:Neurons, Afferent / Q000187:drug effects / Q000648:ultrastructure*; D051381:Rats; D013997:Time Factors
D007797:Laminin