A cortical lectin from the oocytes of Rutilus rutilus stimulates mitogenic activity and release of soluble factors from human lymphocyte cultures and inhibits protein synthesis in a cell-free system.
1. A cortical granule lectin was isolated from vitellogenic oocytes of a bony fish, the roach Rutilus rutilus. 2. The lectin agglutinates human erythrocytes, is specific for L-rhamnose and is composed of different polypeptide subunits. 3. The lectin is the first lectin of animal origin that inhibits protein-synthesis by a rabbit reticulocyte lysate, and is also mitogenic for human lymphocytes.
Licastro
F
F
Department of Experimental Pathology, University of Bologna, Italy.
Barbieri
L
L
Krajhanzi
A
A
Kocourek
J
J
Stripe
F
F
D000328:Adult; D000818:Animals; D002474:Cell-Free System; D002478:Cells, Cultured; D002850:Chromatography, Gel; D003530:Cyprinidae / Q000378:metabolism*; D015232:Dinoprostone / Q000378:metabolism; D005260:Female; D006384:Hemagglutination; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D037102:Lectins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism; D009865:Oocytes / Q000737:chemistry*; D014176:Protein Biosynthesis; D012210:Rhamnose / Q000378:metabolism; D012995:Solubility; D013929:Thromboxane B2 / Q000378:metabolism
D007376:Interleukin-2; D037102:Lectins; D013929:Thromboxane B2; D015232:Dinoprostone; D012210:Rhamnose