Development of human chromosome-specific PCR primers for characterization of somatic cell hybrids.
The human chromosome complement of somatic cell hybrids must be assessed each time the hybrids are grown in culture. We have developed a panel of human-specific oligonucleotide primers for genes that have been mapped to each of the autosomes and to the X chromosome. These primers enable the human chromosome content of hybrids to be assessed rapidly by PCR. The sequence of the primers is presented together with the appropriate conditions for human DNA-specific amplification for each pair.
Abbott
C
C
Department of Genetics & Biometry, University College London, United Kingdom.
Povey
S
S
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002877:Chromosomes, Human; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods*; D013698:Templates, Genetic
D009838:Oligodeoxyribonucleotides