title: Human breast carcinoma cDNA encoding a galactoside-binding lectin homologous to mouse Mac-2 antigen.
abstract: A galactoside-binding lectin (Mr 29,000) has previously been identified in rat, mouse and human tissues. It is an abundant cell-surface component of inflammatory macrophages and their major non-integrin laminin-binding protein. It has also been found in the nucleus of other cell types. Here, we report the cloning and sequencing of a cDNA encoding the human galactoside-binding lectin from a breast carcinoma. The clone encodes a protein of 250 amino acids (aa) that is over 80% identical to its mouse and rat counterparts. The aa sequence has an N-terminal and a C-terminal, 'carbohydrate-binding', domain. The N-terminal domain consists of two parts. The first 41 aa are homologous to a transcription factor, i.e., the serum response factor. The adjacent part (aa 42-106) contains an unusual repeating element, that occurs seven times in human protein compared to nine times in rat and mouse. The C-terminal 'carbohydrate-binding' domain (aa 115-250) shows homology to L-14, another galactoside-binding lectin.
authors.affiliation: Department of Psychiatry, Langley Porter Psychiatric Institute, University of California, San Francisco 94143-0984.
authors.forename: Y
authors.initials: Y
authors.lastname: Oda
authors.forename: H
authors.initials: H
authors.lastname: Leffler
authors.forename: Y
authors.initials: Y
authors.lastname: Sakakura
authors.forename: K
authors.initials: K
authors.lastname: Kasai
authors.forename: S H
authors.initials: SH
authors.lastname: Barondes
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics*; D037161:Galectins; D006388:Hemagglutinins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D012689:Sequence Homology, Nucleic Acid
chemical_list: D002352:Carrier Proteins; D037161:Galectins; D006388:Hemagglutinins