A bacterial model system for chromosomal targeting.
A system that permits efficient site-specific chromosomal targeting of foreign DNA on the Escherichia coli chromosome has been developed, using the FLP site-specific recombination system derived from the yeast 2 mu plasmid. The system demonstrates the feasibility of using site-specific recombination for this purpose, and provides a means to gather information on parameters that may affect chromosomal targeting to guide efforts to establish similar systems in higher eukaryotes. In this model system, the efficiency of integration of foreign DNA is affected by the location of the target site in the chromosome, and the structure of the recombination sites.
Huang
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LC
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706.
Wood
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EA
Cox
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MM
D001483:Base Sequence; D015139:Blotting, Southern; D002876:Chromosomes, Bacterial / Q000502:physiology* / Q000648:ultrastructure; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D011995:Recombination, Genetic
D004269:DNA, Bacterial; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase