title: Modification of myosin light chain phosphorylation in sustained arterial muscle contraction by phorbol dibutyrate.
abstract: The decrease in phosphorylation of the 20 kDa myosin light chain during prolonged K(+)-stimulation of arterial smooth muscle was counteracted by treating this muscle with phorbol dibutyrate. Quantitative phosphopeptide analysis revealed that phorbol dibutyrate induced phosphorylation of serine and threonine residues in the light chain by protein kinase C and phosphorylation of a threonine residue by myosin light chain kinase. The same residues of light chain were also phosphorylated when phorbol dibutyrate was added to muscles pretreated either with the Ca2(+)-channel-blocking agents nifedipine and verapamil, or with the Ca2(+)-chelating agent ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid. The results indicate an interrelationship between protein kinase C and myosin light chain kinase phosphorylated sites of light chain in intact arterial smooth muscle.
authors.affiliation: Department of Physiology and Biophysics, College of Medicine, University of Illinois, Chicago 60612.
authors.forename: A
authors.initials: A
authors.lastname: Rokolya
authors.forename: M
authors.initials: M
authors.lastname: Bárány
authors.forename: K
authors.initials: K
authors.lastname: Bárány
mesh_terms: D000818:Animals; D001158:Arteries / Q000187:drug effects* / Q000502:physiology; D015180:Electrophoresis, Gel, Two-Dimensional; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000201:enzymology; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism*; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D010766:Phosphorylation; D011188:Potassium / Q000378:metabolism; D013552:Swine
chemical_list: D015240:Phorbol 12,13-Dibutyrate; D009219:Myosin-Light-Chain Kinase; D011188:Potassium