Adhesive properties of Haemophilus influenzae to different human cells.
The adhesion of 19 nontypable strains and 3 typable (type b) Haemophilus influenzae to human cells was examined using buccal epithelial cells (BEC), the continuous HEp-2 cell line and human 0 erythrocytes. The strains were classified into three phenotypes, according to their adhesive properties. Phenotype 1 consists of strains that adhere to both buccal epithelial cells and HEp-2 cells. Phenotype 2 consists of strains that adhere to both buccal epithelial cells and erythrocytes and strains belonging to phenotype 3 adhere to none of the three cell types used. Among 22 strains studied, 18 (81.8%) belonged to phenotype 1, 2 (9.1%) to phenotype 2 and 2 (9.1%) to phenotype 3. Fimbriae were observed for 11 (61%) among the 18 adherent strains belonging to phenotype 1. The 7 nonpiliated strains adhered with a significant adhesion index, thus this results would indicate that a non fimbrial adhesin exists.
Jallat
C
C
Laboratoire de Bactériologie-Virologie, Faculté de Pharmacie, Clermont-Ferrand, France.
Darfeuille-Michaud
A
A
Joly
B
B
D001422:Bacterial Adhesion / Q000502:physiology*; D002277:Carcinoma / Q000382:microbiology; D002610:Cheek / Q000382:microbiology; D004912:Erythrocytes / Q000382:microbiology; D006193:Haemophilus influenzae / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D066298:In Vitro Techniques; D007822:Laryngeal Neoplasms / Q000382:microbiology; D008854:Microscopy, Electron; D010641:Phenotype