High-performance liquid chromatographic determination of cephalosporin antibiotics using 0.3 mm I.D. columns.
Four cephalosporins, cefazolin, ceftizoxime, cefaloridine and cefaclor, were determined using a novel microbore high-performance liquid chromatographic system designed to be entirely compatible with direct liquid interfacing (DLI) for mass spectrometric analysis. The chromatographic support was a 5-micron C18 column of 0.3 mm I.D., compared with the usual microbore column diameters of 1-2 mm. The mobile phase contained no buffers or salts which may have caused column blocking or mobile phase crystallization, and the use of a concentration column allowed the injection of large volumes of analyte (up to 500 microliters). The assay was reproducible, the relative standard deviations being less than 20% within-day and between-day for all the drugs. The detection limit for cefaloridine and cefazolin was 1 ng and for cefaclor and ceftizoxime 5 ng.
Moore
C M
CM
Department of Legal Medicine, Nagoya University School of Medicine, Japan.
Sato
K
K
Katsumata
Y
Y
D002433:Cefaclor / Q000032:analysis; D002437:Cefazolin / Q000032:analysis; D015296:Ceftizoxime / Q000032:analysis; D002509:Cephaloridine / Q000032:analysis; D002511:Cephalosporins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D013058:Mass Spectrometry; D011786:Quality Control
D002511:Cephalosporins; D002433:Cefaclor; D015296:Ceftizoxime; D002437:Cefazolin; D002509:Cephaloridine