Effects of vitamin B12 on cadmium toxicity in rats.
The survival rate was high among male Sprague rats treated with 0.15 mg/kg vitamin B12 (cyanocobalamine) after injection of 5 mg/kg CdCl2.H2O (LD50). The cadmium content of the liver and, for some protocols, in the kidney was significantly reduced in survivors. According to UV-V and multinuclear (1H, 13C, 31P, and 113Cd) magnetic resonance spectroscopy no direct interaction seems to take place between cyanocobalamine and CdCl2 in aqueous solution at pH 4.5. An indirect mechanism is put forward to explain the antidotal activity.
Couce
M D
MD
Departamento de Química Inorgánica, Facultad de Farmacia, Universidad de Santiago de Compostela, Galicia, Spain.
Varela
J M
JM
Sánchez
A
A
Casas
J S
JS
Sordo
J
J
López-Rivadulla
M
M
D000818:Animals; D002104:Cadmium / Q000493:pharmacokinetics / Q000633:toxicity*; D019256:Cadmium Chloride; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D007928:Lethal Dose 50; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution; D014805:Vitamin B 12 / Q000494:pharmacology*
D002104:Cadmium; D019256:Cadmium Chloride; D014805:Vitamin B 12