[Skin permeability modifications in vivo in men after application of surfactants. Importance of experimental conditions in the determination of transepidermal insensible water loss].
Trans Epidermal Water Loss (TEWL) was used to evaluate the skin permeability modifications in vivo in man with an evaporimeter Servo Med. Measurements were performed on the forearms of healthy volunteers. At first, influence of the stabilization of the subjects and reproducibility of measurements between morning and evening, and from day to day were determined. The effect on the stratum corneum of 3 surfactants (Tween 60, sodium dodecyl sulfate and cetyl trimethyl ammonium bromide) was studied. The results showed a variability of TEWL during the first 30 minutes, but there were no significant chronobiologic variations. After treatment by surfactants, TEWL increase was in relation with the importance of the aggression and the time after the end of the treatment. 3 hours were necessary to be sure that TEWL increase was only due to surfactants.
Kompaore
F
F
Laboratoire de Pharmacologie, URA CNRS 594, Faculté de Pharmacie, Université Paris XI, Chatenay-Malabry.
Marty
J P
JP
Dupont
C
C
D000328:Adult; D005260:Female; D006801:Humans; D008297:Male; D010539:Permeability / Q000187:drug effects; D012879:Skin Physiological Phenomena; D013501:Surface-Active Agents / Q000008:administration & dosage / Q000494:pharmacology*; D013997:Time Factors; D014870:Water Loss, Insensible / Q000187:drug effects*
D013501:Surface-Active Agents