title: Haemoglobin phenotypes of the wild European mouflon sheep living on the island of Sardinia.
abstract: Haemoglobin (Hb) phenotypes have been studied in 100 wild European mouflons living on the island of Sardinia by means of isoelectric focusing (pH 6.7-7.7 range) of the native tetramers, acid-urea-Triton gel-electrophoresis, and reversed-phase HPLC of globin chains. The result indicates the presence of two beta-globin alleles one of which, corresponding to the beta B, being the most common (f = 0.94). None were carriers of the earlier described Hb A. The new Hb was provisionally named Hb M. Severely anaemic mouflons were able to synthesize Hb C at expense of the Hb B alone, thus suggesting structural and physiological homologies between mouflon beta B and sheep beta A globin genes, and between the newly observed beta M allele and the beta B of the domestic Sardinian sheep.
authors.affiliation: Institute of Physiology of Domestic Animals, Faculty of Veterinary Medicine, University of Sassari, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Naitana
authors.forename: S
authors.initials: S
authors.lastname: Ledda
authors.forename: E
authors.initials: E
authors.lastname: Cocco
authors.forename: L
authors.initials: L
authors.lastname: Manca
authors.forename: B
authors.initials: B
authors.lastname: Masala
mesh_terms: D000483:Alleles; D000740:Anemia / Q000235:genetics / Q000662:veterinary; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005787:Gene Frequency; D006580:Genetic Carrier Screening; D014644:Genetic Variation / Q000235:genetics; D005914:Globins / Q000235:genetics*; D006454:Hemoglobins / Q000235:genetics*; D007525:Isoelectric Focusing; D007558:Italy; D046911:Macromolecular Substances; D008297:Male; D010641:Phenotype; D011110:Polymorphism, Genetic / Q000235:genetics*; D012756:Sheep / Q000235:genetics*; D012757:Sheep Diseases / Q000235:genetics
chemical_list: D006454:Hemoglobins; D046911:Macromolecular Substances; D005914:Globins