title: Changes in rat plasma apolipoproteins and lipoproteins during moderate protein deficiency: potential use in the assessment of nutritional status.
abstract: To test apolipoprotein sensitivity as protein deficiency markers, concomitant evolution of plasma apolipoproteins (apo) and usual nutritional markers (transthyretin, albumin, transferrin) were followed during a 28-d protein restriction in young male Wistar rats. In addition, plasma lipids and chemical composition of lipoproteins were assayed by d 28. The control and the deficient groups were fed 18% and 6% casein diets, respectively. By d 28 the protein-deficient group exhibited hypotriglyceridemia resulting from the decrease in VLDL triacylglycerols; free cholesterol and phospholipids were increased, reflecting the increment in LDL-HLDL1. In plasma total lipoproteins, apo BH, AI and E were not different than controls in the deficient group. Apolipoprotein AIV decreased after d 14 and was significantly less than in controls at d 28. Apolipoprotein BI was considerably reduced by d 14 (43% less) and d 28 (52% less) compared with the control group. Apolipoproteins C + AII were significantly lower in the protein-deficient group by d 14 (43%). By d 28, VLDL apo C were decreased 60% by protein restriction. Transthyretin level was 20% lower in the protein-deficient group by d 7 but returned to control values by d 14. A moderately lower value was observed for albumin by d 7 and d 14 and for transferrin by d 28. These results indicate that, in this model, apo BI and C are more sensitive to protein depletion than usual nutritional markers.
authors.affiliation: Unité de Recherches sur la Nutrition et l'Alimentation, U.1 INSERM, Hôpital Bichat, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Gouache
authors.forename: B
authors.initials: B
authors.lastname: Le Moullac
authors.forename: F
authors.initials: F
authors.lastname: Bleiberg-Daniel
authors.forename: R
authors.initials: R
authors.lastname: Aubert
authors.forename: C
authors.initials: C
authors.lastname: Flament
mesh_terms: D000818:Animals; D001053:Apolipoproteins / Q000097:blood*; D001055:Apolipoproteins B / Q000097:blood; D001056:Apolipoproteins C / Q000097:blood; D001835:Body Weight; D002784:Cholesterol / Q000097:blood; D004435:Eating; D004591:Electrophoresis, Polyacrylamide Gel; D008074:Lipoproteins / Q000097:blood*; D008297:Male; D009752:Nutritional Status; D010743:Phospholipids / Q000097:blood; D011228:Prealbumin / Q000032:analysis; D011488:Protein Deficiency / Q000097:blood*; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000032:analysis; D014168:Transferrin / Q000032:analysis; D014280:Triglycerides / Q000097:blood
chemical_list: D001053:Apolipoproteins; D001055:Apolipoproteins B; D001056:Apolipoproteins C; D008074:Lipoproteins; D010743:Phospholipids; D011228:Prealbumin; D012709:Serum Albumin; D014168:Transferrin; D014280:Triglycerides; D002784:Cholesterol