Cytogenetic and molecular characterization of a newly established neuroblastoma cell line LS.
A new human neuroblastoma cell line (LS) that originated from an abdominal tumor of a 16-month-old girl is presented; it was classified, according to Evans, as being stage III. Morphological (dense-core particles) and biochemical characteristics (dopamine-beta-hydroxylase, acetylcholinesterase, neuron-specific-enolase) confirmed the diagnosis. In addition to a slightly variable modal chromosome number of 48 or 49 (because of marker-chromosomes and autosomal trisomies), cytogenetic analysis revealed two constantly appearing chromosomes with homogeneously stained regions (HSR's). The karyotype remained constant over 50 passages in vitro [49,XX, -12, +der5, + 17, + mar1, + mar2]. Double minutes were a rare phenomenon and appeared only in a few metaphases. In situ hybridization showed that some of the HSR's consisted of amplified N-myc copies. The distribution of the N-myc copies according to in situ hybridization signals along the HSR's was compared with the data of Southern and Northern blotting analyses.
Rudolph
G
G
Abteilung Klinische Genetik, Universität, Tübingen, Federal Republic of Germany.
Schilbach-Stückle
K
K
Handgretinger
R
R
Kaiser
P
P
Hameister
H
H
D000008:Abdominal Neoplasms / Q000235:genetics*; D015152:Blotting, Northern; D015139:Blotting, Southern; D002886:Chromosomes, Human, Pair 17; D004273:DNA, Neoplasm; D005260:Female; D005784:Gene Amplification; D016259:Genes, myc; D006801:Humans; D007223:Infant; D007621:Karyotyping; D009447:Neuroblastoma / Q000235:genetics*; D014314:Trisomy; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm