[Analysis of the reassorted influenza virus clone genome: data for use of ordered selection of RNA segments].
Chicken embryonated eggs were coinfected with influenza A/FPV/Rostock and A/FPV/Weybridge strains. 25 plaque isolates were obtained from the mixed yield and their genetic content was analysed by polyacrylamide gel electrophoresis of H3-uridine-labelled vRNA in a modified gel system. At least 18 clones out of 25 plaque isolates proved to be reassortants; however, only one among them contained the homologous RNA-segments belonging to both parents. The results are in agreement with the concept of an ordered recruitment of vRNA-segments into virions.
Kaverin
N V
NV
Varich
N L
NL
Fudneva
I A
IA
Gubareva
L V
LV
D000818:Animals; D002642:Chick Embryo; D002999:Clone Cells; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D009980:Influenza A virus / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D014771:Virion
D012367:RNA, Viral