Effects of chimeric mutants of human immunodeficiency virus type 1 Rev and human T-cell leukemia virus type I Rex on nucleolar targeting signals.
Two chimeric mutant genes derived from rev of human immunodeficiency virus type 1 and rex of human T-cell leukemia virus type I were constructed to investigate the functions of the nucleolar-targeting signals (NOS) in Rev and Rex proteins. A chimeric Rex protein whose NOS region was substituted with the NOS of Rev was located predominantly in the cell nucleolus and functioned like the wild-type protein in the Rex assay system. However, a chimeric Rev with the NOS of Rex abolished Rev function despite its nucleolar localization. This nonfunctional nucleolar-targeting chimeric protein inhibited the function of both Rex and Rev. In the same experimental conditions, this mutant interfered with the localization of the functional Rex in the nucleolus.
Kubota
S
S
Institute for Virus Research, Kyoto University, Japan.
Nosaka
T
T
Cullen
B R
BR
Maki
M
M
Hatanaka
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002466:Cell Nucleolus / Q000382:microbiology*; D002678:Chimera / Q000235:genetics; D003001:Cloning, Molecular; D004279:DNA, Viral; D015694:Gene Products, rev / Q000235:genetics / Q000378:metabolism; D016370:Gene Products, rex / Q000235:genetics / Q000378:metabolism; D016355:Genes, pX; D015757:Genes, rev; D015497:HIV-1 / Q000235:genetics* / Q000378:metabolism; D015368:Human T-lymphotropic virus 1 / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D021382:Protein Sorting Signals / Q000235:genetics* / Q000378:metabolism; D054321:rev Gene Products, Human Immunodeficiency Virus
D004279:DNA, Viral; D015694:Gene Products, rev; D016370:Gene Products, rex; D021382:Protein Sorting Signals; D054321:rev Gene Products, Human Immunodeficiency Virus