Absorption and disposition of colloidal drug delivery systems. I. High-performance liquid chromatographic (HPLC) analysis of a cyclosporin emulsion.
The amount of cyclosporin A in an oil-in-water emulsion drug delivery system was determined by HPLC. The direct extraction and analysis of an intact emulsion were compared to the analysis of a cracked emulsion and an olive oil solution of the drug. The intra- and interday variability for the intact emulsion was less than 10% from 35 to 150 micrograms/ml, with recovery of 94%. Comparison of the assay results obtained with the emulsion and the olive oil solution gave a highly correlated regression line with a small intercept and a slope close to unity. Thus, the direct extraction and HPLC analysis of drugs in emulsions may be a viable approach to evaluate drug content.
Corvari
S J
SJ
Department of Pharmaceutics, School of Pharmacy, University of Maryland, Baltimore 21201.
Hollenbeck
R G
RG
Leslie
J
J
Plaisance
K I
KI
Young
D
D
D000042:Absorption; D002851:Chromatography, High Pressure Liquid; D003102:Colloids; D003524:Cyclosporins / Q000032:analysis / Q000493:pharmacokinetics*; D004655:Emulsions; D000432:Methanol / Q000032:analysis; D000069463:Olive Oil; D010938:Plant Oils / Q000032:analysis; D012015:Reference Standards
D003102:Colloids; D003524:Cyclosporins; D004655:Emulsions; D000069463:Olive Oil; D010938:Plant Oils; D000432:Methanol