Aminopeptidase A in human placenta and pregnant serum.
The activities of serum aminopeptidases (APs) derived from the placenta rise during pregnancy. To confirm the localization of AP-A, which liberates N-terminal acidic amino acids, in placental tissue and to identify it in the serum of pregnant women, chromatographic separation was performed. When a placental extract was subjected to DEAE cellulose column chromatography, the Glu-MCA degradative activity, or AP-A, was separated into two entities. Inhibition testing revealed that the enzyme eluted in Fraction No. 20 from the DEAE-cellulose column consisted of typical AP-A with a molecular weight of approximately 500,000. When the Glu-MCA degradative activity was observed by filtering pregnant serum through HPLC TSK G3000 gel, the activity was found to be due to serum CAP rather than AP-A. What had been thought to be a biochemicophysiological action inherent in blood AP-A raised additional questions.
Okuyama
T
T
Department of Obstetrics and Gynecology, Tokyo Metropolitan Tsukiji Maternity Hospital, Japan.
Ishiura
S
S
Nojima
M
M
Tsukahara
T
T
Yanagida
M
M
Sugita
H
H
D000626:Aminopeptidases / Q000037:antagonists & inhibitors / Q000097:blood / Q000378:metabolism*; D000900:Anti-Bacterial Agents; D002848:Chromatography, DEAE-Cellulose; D002851:Chromatography, High Pressure Liquid; D010122:Cystinyl Aminopeptidase / Q000097:blood; D005260:Female; D043384:Glutamyl Aminopeptidase; D006146:Guanidines / Q000494:pharmacology; D006801:Humans; D007930:Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D007931:Leucyl Aminopeptidase / Q000097:blood; D009842:Oligopeptides / Q000494:pharmacology; D010455:Peptides; D010618:Phenanthrolines / Q000494:pharmacology; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy / Q000097:blood*; D011691:Puromycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D006146:Guanidines; D009842:Oligopeptides; D010455:Peptides; D010618:Phenanthrolines; D011691:Puromycin; C016977:amastatin; D000626:Aminopeptidases; D007931:Leucyl Aminopeptidase; D010122:Cystinyl Aminopeptidase; D043384:Glutamyl Aminopeptidase; D007930:Leucine; C012211:ubenimex; C025205:1,10-phenanthroline