Determination of zolpidem and zopiclone in serum by capillary column gas chromatography.
A gas chromatographic method using a short, high-resolution capillary column connected to a specific thermoionic detector and requiring a simple and short extraction step without evaporation was developed for the rapid and precise determination of two new hypnotics, zolpidem and zopiclone, in serum at concentrations greater than 5 ng/mL. The assay was linear between 5 and 200 ng/mL, with coefficients of intra- and interassay variation less than 5% for both. The method was validated and then used to analyze zolpidem serum concentrations in nine rabbits after oral administration of 0.5 mg/kg and zopiclone serum concentrations in six patients treated orally with a 7.5-g dose.
Debruyne
D
D
Laboratory of Pharmacology, Caen University Hospital Center, France.
Lacotte
J
J
Hurault de Ligny
B
B
Moulin
M
M
D000818:Animals; D053961:Azabicyclo Compounds; D002849:Chromatography, Gas / Q000379:methods; D006801:Humans; D006993:Hypnotics and Sedatives / Q000097:blood*; D015394:Molecular Structure; D010879:Piperazines / Q000097:blood*; D011725:Pyridines / Q000097:blood*; D011817:Rabbits; D015203:Reproducibility of Results; D000077334:Zolpidem
D053961:Azabicyclo Compounds; D006993:Hypnotics and Sedatives; D010879:Piperazines; D011725:Pyridines; C515050:zopiclone; D000077334:Zolpidem