title: Primary sequences of insulin-like growth factors 1 and 2 isolated from porcine plasma.
abstract: Insulin-like growth factors 1 and 2 were purified from porcine plasma. In addition to the determination of their isoelectric points, the primary structures of both proteins were determined, using low microgram quantities of protein, by the versatile combination of time-of-flight plasma desorption mass spectrometry and automated Edman degradation. Porcine insulin-like growth factor 1 was shown to be homologous to both human and bovine proteins; the type 2 growth factor showed one mutation to both human and bovine type 2 proteins.
authors.affiliation: Department of Biochemistry, Novo Nordisk A/S, Gentofte, Denmark.
authors.forename: S
authors.initials: S
authors.lastname: Bayne
authors.forename: C M
authors.initials: CM
authors.lastname: Hoogerbrugge
authors.forename: J
authors.initials: J
authors.lastname: Thomsen
authors.forename: L
authors.initials: L
authors.lastname: Skriver
authors.forename: S C
authors.initials: SC
authors.lastname: van Buul-Offers
authors.forename: J L
authors.initials: JL
authors.lastname: van den Brande
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D007334:Insulin-Like Growth Factor I / Q000032:analysis* / Q000235:genetics; D007335:Insulin-Like Growth Factor II / Q000032:analysis* / Q000235:genetics; D007525:Isoelectric Focusing; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis; D011994:Recombinant Proteins / Q000032:analysis; D013552:Swine; D014357:Trypsin
chemical_list: D010446:Peptide Fragments; D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D014357:Trypsin