Influence of different surfactants on the separation of von Willebrand factor multimers by use of agarose gel electrophoresis and semi dry blotting technology.
The separation of vWF-multimers by use of sodium dodecyl sulfate (SDS) agarose gel electrophoresis and semi dry blotting instead of tank blotting procedure has been studied. We could demonstrate, that multimers in the range of highest molecular weights could not be visualized using this technique. Therefore we have investigated the influence of different types of anionic surfactants on the separation of vWF-multimers and could show that the hydrophilic/lipophilic balance (HLB) is an important value to characterize the capacity of a surfactant to separate vWF-multimers. The best separation quality was achieved with sodium dodecyl benzene sulfonate (SDBS). This surfactant is a superior alternative to SDS in separating vWF-multimers of the highest molecular weights.
Wüst
T
T
Department of Transfusion Medicine, University of Freiburg, Germany.
Beeser
H
H
Lang
H R
HR
D001557:Benzenesulfonates; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D011489:Protein Denaturation; D013463:Sulfuric Acid Esters; D013501:Surface-Active Agents; D014841:von Willebrand Factor / Q000032:analysis*
D001557:Benzenesulfonates; D013463:Sulfuric Acid Esters; D013501:Surface-Active Agents; D014841:von Willebrand Factor; C001114:dodecylbenzenesulfonic acid