Uneven tissue distribution of minor histocompatibility proteins versus peptides is caused by MHC expression.
Naturally processed minor histocompatibility (H) peptides corresponding to H-4b, H-Y, and an unmapped BALB.B minor H gene were quantified in a relative way in 15 different tissues of male BALB.B mice. For one of these minor H antigens, we could also determine the relative content of the respective protein. For each minor H peptide, an individual tissue distribution was found. Tissues expressing little or no MHC (major histocompatibility complex), like brain, contained only small amounts of minor H peptides or none at all, although the same tissues contained minor H protein in substantial quantities. By contrast, Kb-expressing brains from mice transgenic for Kb under control of the glial acidic protein promoter contained both minor H peptide and protein in high amounts. Thus, the expression of minor H peptides in a given tissue is dependent on coexpression of the restricting MHC class I molecules.
Griem
P
P
Max-Planck-Institut für Biologie Abteilung Immungenetik, Tübingen, Federal Republic of Germany.
Wallny
H J
HJ
Falk
K
K
Rötzschke
O
O
Arnold
B
B
Schönrich
G
G
Hämmerling
G
G
Rammensee
H G
HG
D000818:Animals; D001921:Brain / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D015395:Histocompatibility Antigens Class I / Q000235:genetics; D008285:Major Histocompatibility Complex; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D015778:Minor Histocompatibility Antigens / Q000032:analysis / Q000235:genetics*; D009928:Organ Specificity; D012867:Skin / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology
D015395:Histocompatibility Antigens Class I; D015778:Minor Histocompatibility Antigens