Monoclonal antibodies to the soluble human IL-6 receptor: affinity purification, ELISA, and inhibition of ligand binding.
Soluble IL-6 receptor (IL-6-R) purified to homogeneity from normal human urine was used for immunization of mice and rabbits. Spleen cells derived from a mouse showing a high binding titer to IL-6-R in an inverted solid phase radioimmunoassay (IsRIA) and in a Western blotting analysis were fused to mouse myeloma cells. The hybridomas were screened by the IsRIA, and 30 positive clones were isolated and characterized. They were suitable for affinity purification of the IL-6-R and for its detection by Western blot analysis, by ELISA and by sandwich type sRIA. Most of them inhibited the binding of labeled IL-6-R to IL-6 in a solid phase RIA.
Novick
D
D
Weizmann Institute of Science, Dpt. of Molecular Genetics and Virology, Rehovot, Israel.
Engelmann
H
H
Revel
M
M
Leitner
O
O
Rubinstein
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D001667:Binding, Competitive; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D015850:Interleukin-6 / Q000378:metabolism*; D051379:Mice; D011817:Rabbits; D011863:Radioimmunoassay; D011971:Receptors, Immunologic / Q000276:immunology* / Q000378:metabolism; D019947:Receptors, Interleukin-6; D012995:Solubility
D000911:Antibodies, Monoclonal; D015850:Interleukin-6; D011971:Receptors, Immunologic; D019947:Receptors, Interleukin-6