title: Development of human chromosome-specific PCR primers for characterization of somatic cell hybrids.
abstract: The human chromosome complement of somatic cell hybrids must be assessed each time the hybrids are grown in culture. We have developed a panel of human-specific oligonucleotide primers for genes that have been mapped to each of the autosomes and to the X chromosome. These primers enable the human chromosome content of hybrids to be assessed rapidly by PCR. The sequence of the primers is presented together with the appropriate conditions for human DNA-specific amplification for each pair.
authors.affiliation: Department of Genetics & Biometry, University College London, United Kingdom.
authors.forename: C
authors.initials: C
authors.lastname: Abbott
authors.forename: S
authors.initials: S
authors.lastname: Povey
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002877:Chromosomes, Human; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods*; D013698:Templates, Genetic
chemical_list: D009838:Oligodeoxyribonucleotides