title: Candida albicans serotype A strains grow in yeast extract-added Sabouraud liquid medium at pH 2.0, elaborating mannans without beta-1,2 linkage and phosphate group.
abstract: Cultivation of three Candida albicans strains, NIH A-207, J-1012, and NIH B-792, abbreviated as A-, J-, and B-strains, respectively, in yeast extract-enrich Sabouraud liquid medium at pH 2.0 provided the following findings, i.e., the two former strains belonging to serotype A were able to grow in this medium in almost the same rates as those in the same medium of pH 5.9, while B-strain cells did not proliferate under the former condition. The cells of A- and J-strains cultivated at pH 2.0 did not undergo agglutination with the factor serum 6 in a commercially available factor serum kit, Candida Check, corresponding to C. albicans serotype A-specific epitope. It was also revealed by 1H-13C correlation spectra of the mannans isolated from the cells of A- and J-strains contained neither phosphate group nor beta-1,2-linked mannopyranose unit, although these mannans retained non-reducing terminal alpha-1,3 linked mannopyranose units, providing a substantiating evidence that the serotype A-specific epitope contains a non-reducing terminal beta-1,2-linked mannopyranose unit.
authors.affiliation: Second Department of Hygienic Chemistry, Tohoku College of Pharmacy, Miyagi, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
authors.forename: P
authors.initials: P
authors.lastname: Giummelly
authors.forename: S
authors.initials: S
authors.lastname: Takahashi
authors.forename: M
authors.initials: M
authors.lastname: Ishida
authors.forename: J
authors.initials: J
authors.lastname: Sato
authors.forename: M
authors.initials: M
authors.lastname: Takaku
authors.forename: Y
authors.initials: Y
authors.lastname: Nishidate
authors.forename: N
authors.initials: N
authors.lastname: Shibata
authors.forename: Y
authors.initials: Y
authors.lastname: Okawa
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
mesh_terms: D002176:Candida albicans / Q000145:classification / Q000254:growth & development* / Q000378:metabolism; D002236:Carbohydrate Conformation; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D010766:Phosphorylation; D013045:Species Specificity
chemical_list: D003470:Culture Media; D008351:Mannans