Wheat germ initiation factors 4F and (iso)4F interact differently with oligoribonucleotide analogues of rabbit alpha-globin mRNA.
The binding of capped oligoribonucleotide analogues of the 5' terminus of rabbit alpha-globin mRNA to wheat germ protein synthesis initiation factors eIF-4F and eIF-(iso)4F was measured by direct fluorescence techniques. An analysis of the equilibrium association constants (Keq) indicates that both eIF-4F and eIF-(iso)4F recognize primarily the m7G cap structure but differ in the recognition of other structural features. eIF-4F is sensitive to the position and sequence of hairpin structures within the oligoribonucleotide, while eIF-(iso)4F shows a preference for linear sequences. These differences suggest that wheat germ eIF-4F and eIF-(iso)4F may have discriminatory activity for mRNA recognition.
Carberry
S E
SE
Department of Chemistry, Hunter College, City University of New York, New York 10021.
Goss
D J
DJ
D000818:Animals; D001483:Base Sequence; D039562:Eukaryotic Initiation Factor-4F; D039642:Eukaryotic Initiation Factors; D005453:Fluorescence; D005914:Globins / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000235:genetics; D010448:Peptide Initiation Factors / Q000235:genetics*; D012315:RNA Caps / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits; D012639:Seeds / Q000235:genetics; D014908:Triticum / Q000235:genetics*
D039562:Eukaryotic Initiation Factor-4F; D039642:Eukaryotic Initiation Factors; D009841:Oligonucleotides; D010448:Peptide Initiation Factors; D012315:RNA Caps; D012333:RNA, Messenger; C031799:eIF-4B; D005914:Globins