Quantum yields for the cyclization and configurational isomerization of 4E,15Z-bilirubin.
Extraction of a solution of bilirubin configurational isomers in chloroform with an aqueous solution of human serum albumin was found to remove selectively the 4Z,15E-isomer. This phenomenon was used to develop a method for the purification of the 4E,15Z-isomer of bilirubin. The quantum yield for the cyclization and configurational isomerization of the 4E,15Z-isomer bound to a molar excess of human serum albumin was measured at 450 and 510 nm. The quantum yield for cyclization to form lumirubin was 0.12 and 0.19 at 450 and 510 nm, respectively. The quantum yield for configurational isomerization to form 4Z,15Z-bilirubin was 0.03 and 0.05 at 450 and 510 nm. An analysis of previously published data on the quantum yield for the formation of lumirubin from 4Z, 15Z-bilirubin bound to human serum albumin suggests that all of the formation of lumirubin may occur via consecutive photochemical processes with the 4E,15Z-isomer as an intermediate.
Ennever
J F
JF
Department of Pediatrics, Rainbow Babies and Children's Hospital, Case Western Reserve University, Cleveland, OH 44106.
Dresing
T J
TJ
D001663:Bilirubin / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007231:Infant, Newborn; D007536:Isomerism; D008165:Luminol; D015394:Molecular Structure; D011789:Quantum Theory
D008165:Luminol; D001663:Bilirubin