Eukaryotic DNA primase. Abortive synthesis of oligoadenylates.
Calf thymus DNA polymerase alpha-primase, human placenta DNA polymerase alpha-primase and human placenta DNA primase synthesized oligoriboadenylates of a preferred length of 2-10 nucleotides and multimeric oligoribonucleotides of a modal length of about 10 monomers on a poly(dT) template. The dimer and trimer were the prevalent products of the polymerization reaction. However, only the oligonucleotides from heptamers to decamers were elongated efficiently by DNA polymerase alpha.
Podust
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VN
Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk.
Vladimirova
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OV
Manakova
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EN
Lavrik
O I
OI
D000227:Adenine Nucleotides / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D004257:DNA Polymerase II / Q000378:metabolism*; D019915:DNA Primase; D006801:Humans; D009843:Oligoribonucleotides / Q000378:metabolism*; D010920:Placenta / Q000201:enzymology*; D011071:Poly T / Q000378:metabolism; D012316:RNA Nucleotidyltransferases / Q000378:metabolism*; D013698:Templates, Genetic
D000227:Adenine Nucleotides; D009843:Oligoribonucleotides; D011071:Poly T; C023505:2',5'-oligoadenylate; D000255:Adenosine Triphosphate; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases; D004257:DNA Polymerase II