In vitro secretion of prostaglandins from endometrium of postpartum beef cows expected to have short or normal luteal phases.
The objective of this study was to characterize endometrial secretion (in vitro) of prostaglandin F (PGF), 15-keto-13,14-dihydro-prostaglandin F2 alpha (PGFM), prostaglandin E2 (PGE2), and 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha) on Day 5 following the first postpartum estrus of cows anticipated to have a short compared to a normal estrous cycle. Twenty-seven beef cows were randomly assigned into four groups. The Short Cycle (n = 6; control) and Short Cycle/Explant (n = 8; endometrial explants) groups had their calves weaned at 30-32 days postpartum. The Normal Cycle (n = 5, control) and Normal Cycle/Explant (n = 8; endometrial explants) groups received norgestomet (progestin) implants for 9 days beginning 21-23 days postpartum, and calves were weaned at implant insertion. Estrous cycle length (mean +/- SE; p less than 0.01) for the Short Cycle group was 11.5 +/- 1.9 days compared to 18.8 +/- 0.6 days for the Normal Cycle group. On Day 5 following the first postpartum estrus, cows in the Short Cycle/Explant and Normal Cycle/Explant groups were hysterectomized, and endometrial explants were incubated in Earle's Balanced Salt solution/Medium 199 for 90 min with or without arachidonic acid (AA) in the presence of three levels of oxytocin. Mean concentrations of PGF and PGFM were combined to obtain a value for total PGF. Concentrations of total PGF, PGE2 (from explants without AA treatment), and 6-keto-PGF1 alpha in medium of the Short Cycle/Explant group were higher (p less than 0.01) than in medium of the Normal Cycle/Explant group.(ABSTRACT TRUNCATED AT 250 WORDS)
Zollers
W G
WG
Department of Dairy Science, University of Missouri, Columbia 65211.
Garverick
H A
HA
Youngquist
R S
RS
Ottobre
J S
JS
Silcox
R W
RW
Copelin
J P
JP
Smith
M F
MF
D000818:Animals; D002417:Cattle; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D008183:Luteal Phase / Q000502:physiology; D049590:Postpartum Period / Q000502:physiology*; D011374:Progesterone / Q000097:blood; D011453:Prostaglandins / Q000378:metabolism*; D011460:Prostaglandins F / Q000378:metabolism; D013997:Time Factors
D011453:Prostaglandins; D011460:Prostaglandins F; D011374:Progesterone