Carcinogenicity of p-chloroaniline in rats and mice.
p-Chloroaniline (PCA), a dye intermediate, was evaluated for potential long-term toxicity and carcinogenicity. Groups of 50 F344/N rats of each sex were given by gavage PCA hydrochloride in deionized water at doses of 0, 2, 6 or 18 mg/kg body weight, 5 days/wk for 103 wk. Groups of 50 male and female B6C3F1 mice of each sex were given 0, 3, 10 or 30 mg/kg on the same schedule. In general, body weights and survival were unaffected by PCA administration. In rats the group given 18 mg/kg had mild haemolytic anaemia and slight increases in methaemoglobin at various times during the study. Fibrosis of the spleen was significantly increased in all PCA-treated groups of male rats and in the 18-mg/kg group of female rats. Sarcomas of the spleen occurred in male rats, their incidence being 0/49, 1/50, 3/50 and 38/50 in control low-, mid- and high-dose groups, respectively. There was a slightly increased incidence of pheochromocytomas of the adrenal gland in both male and female rats. Dosed groups of male mice had increased incidences of hepatocellular adenomas or carcinomas (11/50, 21/49, 20/50 and 21/50 in controls, low- mid- and high-dose groups, respectively). Haemangiosarcomas of the liver or spleen were also increased in the high-dose group (incidences of 4/50, 4/49, 1/50 and 10/50 in controls, low-, mid- and high-dose groups, respectively). In conclusion, PCA was carcinogenic in male rats and male mice.
Chhabra
R S
RS
National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.
Huff
J E
JE
Haseman
J K
JK
Elwell
M R
MR
Peters
A C
AC
D000236:Adenoma / Q000139:chemically induced; D000310:Adrenal Gland Neoplasms / Q000139:chemically induced; D000814:Aniline Compounds / Q000633:toxicity*; D000818:Animals; D002277:Carcinoma / Q000139:chemically induced; D005260:Female; D005355:Fibrosis; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced; D008297:Male; D051379:Mice; D009374:Neoplasms, Experimental / Q000139:chemically induced*; D010673:Pheochromocytoma / Q000139:chemically induced; D051381:Rats; D011916:Rats, Inbred F344; D012513:Sarcoma, Experimental / Q000139:chemically induced; D013154:Spleen / Q000473:pathology; D013160:Splenic Neoplasms / Q000139:chemically induced
D000814:Aniline Compounds; C004658:4-chloroaniline