Cytoskeleton in TFG-beta- and bFGF-modulated endothelial monolayer repair.
Endothelial cell proliferation and migration in vitro is depressed by transforming growth factor beta (TFG-beta) and enhanced by basic fibroblast growth factor (bFGF) treatment. This study examines interactions between cytoskeletal changes and cell proliferation in regenerating endothelial monolayers treated with bFGF, TFG-beta, and both factors. As previously described by others, monolayer regeneration is enhanced by bFGF and reduced by TFG-beta. Endothelial cell morphology is altered by TFG-beta treatment. Cells lose their cobblestone appearance and assume a pleomorphic shape. Actin microfilament staining is modified in both intact and regenerating TFG-beta-treated monolayers as well. There is a loss of dense peripheral band staining and an enhancement in staining intensity of cytoplasmic stress fibers. No such alterations are seen in bFGF-treated cultures. Cell proliferation at the wound edge, as indicated by bromodeoxyuridine incorporation, is inhibited by TGF-beta. Although monolayer repair is modulated by growth factor treatment, centrosome reorientation and microtubule staining patterns are not altered by either factor. Thus these factors appear to have effects on a mechanism(s) other than centrosome reorientation which may be involved in repair of denuded endothelial monolayers.
Coomber
B L
BL
Department of Zoology, University of Western Ontario, London, Canada.
D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D016222:Fibroblast Growth Factor 2 / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D008870:Microtubules / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D016212:Transforming Growth Factor beta / Q000502:physiology*
D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2