The killing of cultured hepatocytes by N-acetyl-p-benzoquinone imine (NAPQI) as a model of the cytotoxicity of acetaminophen.
The killing of isolated hepatocytes by N-acetyl-p-benzoquinone imine (NAPQI), the major metabolite of the oxidation of the hepatotoxin acetaminophen, has been studied previously as a model of liver cell injury by the parent compound. Such studies assume that the toxicity of acetaminophen is mediated by NAPQI and that treatment with exogenous NAPQI reproduces the action of the endogenously produced product. The present study tested these assumptions by comparing under identical conditions the toxicity of acetaminophen and NAPQI. The killing of hepatocytes by acetaminophen was mediated by oxidative injury. Thus, it depended on a cellular source of ferric iron; was potentiated by 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU), an inhibitor of glutathione reductase; and was sensitive to antioxidants. By contrast, the cytotoxicity of NAPQI was not prevented by chelation of ferric iron; was unaffected by BCNU; and was insensitive to antioxidants. Thus, the killing of cultured hepatocytes by NAPQI occurs by a mechanism different from that of acetaminophen. The killing by NAPQI was preceded by a collapse of the mitochondrial membrane potential and a depletion of ATP. Monensin potentiated the cell killing, and extracellular acidosis prevented it. These manipulations are characteristic of the toxicity of mitochondrial poisons, and are without effect on the depletion of ATP and the loss of mitochondrial energization. Thus, mitochondrial de-energization by a mechanism unrelated to oxidative stress is a likely basis of the cell killing by NAPQI. It is concluded that treatment of cultured hepatocytes with NAPQI does not model the cytotoxicity of acetaminophen in these cells.
Harman
A W
AW
Department of Pathology, Thomas Jefferson University, Philadelphia, PA 19107.
Kyle
M E
ME
Serroni
A
A
Farber
J L
JL
D000082:Acetaminophen / Q000633:toxicity*; D000138:Acidosis / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001571:Benzoflavones / Q000494:pharmacology; D016227:Benzoquinones / Q000633:toxicity*; D002330:Carmustine / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003676:Deferoxamine / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D005980:Glutathione Reductase / Q000037:antagonists & inhibitors; D007097:Imines / Q000633:toxicity*; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D008954:Models, Biological; D008985:Monensin / Q000494:pharmacology; D010084:Oxidation-Reduction; D010655:Phenylenediamines / Q000494:pharmacology; D051381:Rats; D019324:beta-Naphthoflavone
D001571:Benzoflavones; D016227:Benzoquinones; D007097:Imines; D010655:Phenylenediamines; D000082:Acetaminophen; D019324:beta-Naphthoflavone; D000255:Adenosine Triphosphate; D008985:Monensin; C004845:N,N'-diphenyl-4-phenylenediamine; D005980:Glutathione Reductase; C028473:N-acetyl-4-benzoquinoneimine; D005978:Glutathione; D003676:Deferoxamine; D002330:Carmustine