Cloning and expression of an Aplysia K+ channel and comparison with native Aplysia K+ currents.
We describe here the cloning of the Aplysia K+ channel AK01a.AK01a codes for a protein of 515 amino acids, shows considerable homology to other cloned potassium channels, and can be classified as a member of the ShakerK+ channel family. Expression of the AK01a channel in Xenopus oocytes produces a rapidly inactivating outward potassium current (IAK01a) resembling the A-type currents of Drosophila Shaker. Gating for this current is shifted to potentials considerably more positive than the traditional A-currents of Aplysia; we have, however, identified a novel transient potassium current (IAdepoI) in a subset of Aplysia neurons that has similar gating and pharmacological properties to IAK01a.
Pfaffinger
P J
PJ
Howard Hughes Medical Institute, Department of Physiology and Biophysics, Columbia University, College of Physicians and Surgeons, New York, New York 10032.
Furukawa
Y
Y
Zhao
B
B
Dugan
D
D
Kandel
E R
ER
D000595:Amino Acid Sequence; D000818:Animals; D001048:Aplysia / Q000378:metabolism*; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004563:Electrochemistry; D007700:Kinetics; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D015221:Potassium Channels / Q000235:genetics* / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D014018:Tissue Distribution; D014981:Xenopus / Q000378:metabolism
D015221:Potassium Channels