Sodium arsenite induces ATP depletion and mitochondrial damage in HeLa cells.
Our present results show that treatment with sodium arsenite apparently decreases cellular ATP levels in a dose- and time-dependent manner in HeLa S-3 cells. The reduction in ATP induced by sodium arsenite was possibly through mitochondrial damage, since treatment with sodium arsenite resulted in reduction of rhodamine 123 accumulation and disruption of the structure of the cristae in mitochondria. However, all of these changes could be reversed by removing sodium arsenite from the culture medium. The levels of ATP depletion were correlated with the killing effects of sodium arsenite in HeLa S-3 cells.
Yih
L H
LH
Institute of Radiation Biology, National Tsing-Hua University, Taiwan, Republic of China.
Huang
H M
HM
Jan
K Y
KY
Lee
T C
TC
D000255:Adenosine Triphosphate / Q000378:metabolism*; D001151:Arsenic / Q000494:pharmacology*; D018053:Arsenites; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000187:drug effects* / Q000473:pathology; D017670:Sodium Compounds
D018053:Arsenites; D017670:Sodium Compounds; C017947:sodium arsenite; D000255:Adenosine Triphosphate; D001151:Arsenic