[Immunohistochemical localization of calcitonin gene-related peptide (CGRP) and neuropeptide Y (NPY) in the cochlea and middle ear of the guinea pig].
The avidin-biotin peroxidase complex immunostaining method combined with the glucose oxidase-nickel (ABC-GON) technique was applied to the guinea pig cochlea and middle ear, the fine structure of CGRP-containing fibers in the organ of Corti was also observed. The results demonstrated that the CGRP-containing fibers in the cochlea belonged to both the medial and lateral efferent systems of olivocochlear bundle. The CGRP positive nerve endings on outer hair cell base formed synaptic contacts with the cell body. No NPY-containing fibers were found in the cochlea. Numerous CGRP and NPY-containing fibers of a similar distribution pattern and density were seen in the middle ear mucosa. The pars tensa of ear drum was also innervated by moderate CGRP-containing fibers and a few NPY-containing fibers. Occasionally, the CGRP-containing fibers were observed along the radial fibers in the pars tensa. The role of two neuropeptides in the cochlea and middle ear are discussed.
Wu
W
W
Xijing Hospital of 4th Military Medical University, Xi'an.
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis*; D003051:Cochlea / Q000378:metabolism*; D004432:Ear, Middle / Q000378:metabolism*; D006168:Guinea Pigs; D007150:Immunohistochemistry; D008297:Male; D009478:Neuropeptide Y / Q000032:analysis*; D009925:Organ of Corti / Q000378:metabolism
D009478:Neuropeptide Y; D015740:Calcitonin Gene-Related Peptide