title: Beta-lactamase is encoded on plasmid pACK3 in Staphylococcus simulans biovar staphylolyticus.
abstract: Staphylococcus simulans biovar staphylolyticus, the lysostaphin-producing organism, contains five plasmids designated pACK1 through pACK5. Hybridization analysis using cloned beta-lactamase gene (bla) as probe and characterization of cured strains revealed that bla resides on pACK3 rather than on the lysostaphin endopeptidase plasmid (pACK1) as reported by others.
authors.affiliation: Department of Microbiology, University of Alabama, Tuscaloosa 35487.
authors.forename: H E
authors.initials: HE
authors.lastname: Heath
authors.forename: L S
authors.initials: LS
authors.lastname: Heath
authors.forename: K E
authors.initials: KE
authors.lastname: Rose
authors.forename: G L
authors.initials: GL
authors.lastname: Sloan
mesh_terms: D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry; D010450:Endopeptidases / Q000235:genetics; D005798:Genes, Bacterial; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D015183:Restriction Mapping; D013210:Staphylococcus / Q000235:genetics*; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D010450:Endopeptidases; C021524:lysostaphin endopeptidase; D001618:beta-Lactamases