Ligation with T4 RNA ligase of an oligodeoxyribonucleotide to covalently-linked cross-sectional base-pair analogues of short, normal, and long dimensions.
Compounds that are covalent analogues of nucleic acid base pairs of normal, long, and short C1' to C1' dimensions [B. Devadas and N.J. Leonard (1990) J. Am. Chem. Soc., 112, 3125-3135.] have been added to the oligodeoxyribonucleotide d(A)6 with bacteriophage T4 RNA ligase as a prelude to placing them at defined loci within nucleic acid duplexes. Analogue cross sections that represent a normal Watson-Crick base pair as well as a pyrimidine-pyrimidine and a purine-purine apposition were ligated in modest yields (approximately 20%) to the oligonucleotide. Ligation conditions were optimized for each analogue, and the cross section was joined to only a single oligonucleotide in each case. The structures of the ligated products were proved by HPLC, enzymatic degradation, and spectroscopic analyses.
Petric
A
A
Department of Chemistry and Chemical Technology, E. Kardelj University, Ljubljana, Yugoslavia.
Bhat
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B
Leonard
N J
NJ
Gumport
R I
RI
D002851:Chromatography, High Pressure Liquid; D011088:DNA Ligases / Q000378:metabolism*; D006860:Hydrogen Bonding; D008956:Models, Chemical; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010766:Phosphorylation
D009838:Oligodeoxyribonucleotides; D011088:DNA Ligases