title: Imaging of calcium transients in skeletal muscle fibers.
abstract: Epifluorescence images of Ca2+ transients elicited by electrical stimulation of single skeletal muscle fibers were studied with fast imaging techniques that take advantage of the large fluorescence signals emitted at relatively long wavelengths by the dyes fluo-3 and rhod-2 in response to binding of Ca2+ ions, and of the suitable features of a commercially available CCD video camera. The localized release of Ca2+ in response to microinjection of InsP3 was also monitored to demonstrate the adequate space and time resolutions of the imaging system. The time resolution of the imager system, although limited to the standard video frequency response, still proved to be adequate to investigate the fast Ca2+ release process in skeletal muscle fibers at low temperatures.
authors.affiliation: Department of Physiology, University of California, Los Angeles 90024.
authors.forename: J
authors.initials: J
authors.lastname: Vergara
authors.forename: M
authors.initials: M
authors.lastname: DiFranco
authors.forename: D
authors.initials: D
authors.lastname: Compagnon
authors.forename: B A
authors.initials: BA
authors.lastname: Suarez-Isla
mesh_terms: D000814:Aniline Compounds; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D005456:Fluorescent Dyes; D006575:Heterocyclic Compounds, 3-Ring; D066298:In Vitro Techniques; D015544:Inositol 1,4,5-Trisphosphate / Q000008:administration & dosage / Q000494:pharmacology; D008845:Microinjections; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D009132:Muscles / Q000187:drug effects / Q000502:physiology*; D011892:Rana catesbeiana; D014966:Xanthenes
chemical_list: D000814:Aniline Compounds; D005456:Fluorescent Dyes; D006575:Heterocyclic Compounds, 3-Ring; D014966:Xanthenes; C068483:rhod-2; C059715:Fluo-3; D015544:Inositol 1,4,5-Trisphosphate; D002118:Calcium