Isolation of group 2 aerotolerant Campylobacter species from Thai children with diarrhea.
Campylobacter species were isolated from 93 (15%) of 631 Thai children with diarrhea using the membrane filter technique on nonselective blood agar incubated at 37 degrees C. Campylobacter jejuni was isolated from 62 (10%), Campylobacter coli from 14 (2%), and atypical campylobacters from 17 (3%). The 17 atypical strains were first characterized biochemically and by dot blot DNA hybridization. Catalase-negative strains also were characterized by DNA hybridization and ribotype pattern. One strain was a catalase-negative "Campylobacter upsaliensis" and another was a nitrate-negative Campylobacter jejuni doylei. Fifteen isolates were aerotolerant strains most closely resembling Campylobacter cryaerophila or "C. upsaliensis" by dot hybridization. These aerotolerant strains, designated group 2 ("Campylobacter butzleri"), had ribotypes distinct from C. cryaerophila and have previously been shown to be related by DNA hybridization at the species level to the group 2 aerotolerant Campylobacter type strain (D2686). Group 2 aerotolerant Campylobacter were the atypical Campylobacter species most frequently isolated from Thai children with diarrhea.
Taylor
D N
DN
Department of Bacterial Diseases, Walter Reed Army Institute of Research, Washington, DC 20307-5100.
Kiehlbauch
J A
JA
Tee
W
W
Pitarangsi
C
C
Echeverria
P
P
D002167:Campylobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002169:Campylobacter Infections / Q000382:microbiology*; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D003967:Diarrhea / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D006801:Humans; D007223:Infant; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D013785:Thailand
D004269:DNA, Bacterial