title: Doping control of testosterone and human chorionic gonadotrophin: a case study.
abstract: Doping control for testosterone and human Chorionic Gonadotrophin (hCG) requires special attention as a difference must be made between the endogenous and exogenous origin of both substances. The detection of exogenous testosterone is based on the ratio of testosterone- to epitestosterone-glucuronide (T/E) in urine. The problems with this ratio are discussed. For hCG analysis in urine the utilization of sandwich-type hCG specific assays instead of hCG/hCG beta competitive assays is recommended. A case study in which an athlete self-administered testosterone and hCG before a competition is described. The T/E ratio and hCG concentration in urine were followed during this period of self-administration. The results demonstrate the relevance of the T/E ratio and of the selected hCG assay. The ratio of testosterone to human Luteinizing Hormone (T/hLH) in serum also indicated the use of hormones. Although the athlete's urine was negative for exogenous testosterone directly after competition, he would have been found positive for hCG.
authors.affiliation: Netherlands Institute for Drugs and Doping Research, University of Utrecht.
authors.forename: D
authors.initials: D
authors.lastname: de Boer
authors.forename: E G
authors.initials: EG
authors.lastname: de Jong
authors.forename: J M
authors.initials: JM
authors.lastname: van Rossum
authors.forename: R A
authors.initials: RA
authors.lastname: Maes
authors.forename: E G
authors.initials: EG
authors.lastname: De Jong
mesh_terms: D006063:Chorionic Gonadotropin / Q000008:administration & dosage* / Q000097:blood / Q000652:urine; D004300:Doping in Sports / Q000517:prevention & control*; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000592:standards; D008297:Male; D012651:Self Medication; D013739:Testosterone / Q000008:administration & dosage* / Q000097:blood / Q000652:urine
chemical_list: D006063:Chorionic Gonadotropin; D013739:Testosterone