title: A transforming growth factor beta (TGF-beta) receptor from human placenta exhibits a greater affinity for TGF-beta 2 than for TGF-beta 1.
abstract: Affinity-labeling techniques have been used to identify three types of high-affinity receptors for transforming growth factor beta (TGF-beta) on the surface of many cells in culture. Here we demonstrate that membrane preparations from tissue sources may also be used as an alternative system for studying the binding properties of TGF-beta receptors. Using a chemical cross-linking technique with 125I-TGF-beta 1 and 125I-TGF-beta 2 and bis(sulfosuccinimidyl)suberate (BS3), we have identified and characterized two high-affinity binding components in membrane preparations derived from human term placenta. The larger species, which migrates as a diffuse band of molecular mass 250-350 kDa on sodium dodecyl sulfate-polyacrylamide electrophoresis gels, is characteristic of the TGF-beta receptor type III, a proteoglycan containing glycosaminoglycan (GAG) chains of chondroitin and heparan sulfate. The smaller species of molecular mass 140 kDa was identified as the core glycoprotein of this type III receptor by using the techniques of enzymatic deglycosylation and peptide mapping. Competition experiments, using 125I-TGF-beta 1 or 125I-TGF-beta 2 and varying amounts of competing unlabeled TGF-beta 1 or TGF-beta 2, revealed that both the placental type III proteoglycan and its core glycoprotein belong to a novel class of type III receptors that exhibit a greater affinity for TGF-beta 2 than for TGF-beta 1. This preferential binding of TGF-beta 2 to placental type III receptors suggests differential roles for TGF-beta 2 and TGF-beta 1 in placental function.
authors.affiliation: National Research Council of Canada, Biotechnology Research Institute, Montreal, Quebec.
authors.forename: E J
authors.initials: EJ
authors.lastname: Mitchell
authors.forename: M D
authors.initials: MD
authors.lastname: O'Connor-McCourt
mesh_terms: D000345:Affinity Labels; D001345:Autoradiography; D001667:Binding, Competitive; D004591:Electrophoresis, Polyacrylamide Gel; D066246:ErbB Receptors / Q000302:isolation & purification* / Q000378:metabolism; D005260:Female; D006031:Glycosylation; D006801:Humans; D008024:Ligands; D010449:Peptide Mapping; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: D000345:Affinity Labels; D008024:Ligands; D016212:Transforming Growth Factor beta; D066246:ErbB Receptors