title: Resolution of the conformational distribution and dynamics of a flexible molecule using frequency-domain fluorometry.
abstract: We report the first resolution of both the conformational distribution and end-to-end diffusion coefficient of a flexible molecule. This molecular information was recovered using only the donor intensity decay in a single solvent at a single viscosity, as observed by the technique of frequency-domain fluorometry. This technique can be extended to measurements of structural fluctuations of biological macromolecules.
authors.affiliation: University of Maryland, School of Medicine, Department of Biological Chemistry, Baltimore 21201.
authors.forename: J R
authors.initials: JR
authors.lastname: Lakowicz
authors.forename: J
authors.initials: J
authors.lastname: Kuśba
authors.forename: W
authors.initials: W
authors.lastname: Wiczk
authors.forename: I
authors.initials: I
authors.lastname: Gryczynski
authors.forename: H
authors.initials: H
authors.lastname: Szmacinski
authors.forename: M L
authors.initials: ML
authors.lastname: Johnson
mesh_terms: D008433:Mathematics; D008962:Models, Theoretical; D008968:Molecular Conformation; D011336:Probability; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence / Q000379:methods