Identification of novel single-stranded d(TC)n binding proteins in several mammalian species.
A group of single-stranded d(TC)n specific binding proteins has been detected in the nuclear extracts of several mammalian species that included mouse, human, African green monkey, chimpanzee, and Chinese muntjac. Southwestern analysis of 500 mM KCI nuclear extracts has shown that these proteins cluster in a similar size range, 55.5 to 57 kD. An additional 54 kD band was present for the three primate species examined. The single-stranded d(TC)n binding activity was confirmed with bandshift assay. Specific double-stranded binding activity for duplex d(TC)n.d(GA)n or single-stranded d(GA)n was not detected. The conservation of size distribution and d(TC)n-binding activity across the species examined indicates that this class of single-stranded binding proteins may have an important biological function in vivo.
Yee
H A
HA
Department of Medical Biochemistry, Faculty of Medicine, University of Calgary, Alberta, Canada.
Wong
A K
AK
van de Sande
J H
JH
Rattner
J B
JB
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D002460:Cell Line; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins / Q000032:analysis; D003670:Deer; D004591:Electrophoresis, Polyacrylamide Gel; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000032:analysis; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry*; D002679:Pan troglodytes; D013045:Species Specificity; D013379:Substrate Specificity; D014764:Viral Proteins / Q000032:analysis
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D009692:Nucleic Acid Heteroduplexes; D014764:Viral Proteins; C060035:gp32 protein, Enterobacteria phage T4