The biological function of rabbit blastocyst peptides (RBPs).
The inhibitory effect of rabbit blastocystic peptides (RBPs) on lymphocyte transformation was studied by the method of measuring the incorporation of 3H-thymidine into DNA. The results indicated that RBPs inhibited PHA-stimulated rat and human lymphocyte transformation in vitro. In the concentration ranging from 40 to 200 micrograms/2.5 ml, the inhibition was dose-dependent. No obvious inhibitory action was found with hCG (16-128) IU/2.5 ml), progesterone (250 ng-1 microgram/2.5 ml) and pregnant rabbit serum. It was further demonstrated that RBPs at a higher dosage (200 micrograms/ml) was inhibitory to PGF2 alpha secretion. On the other hand, the 3H-leucine incorporation of rabbit endometrium was enhanced by these peptides, and this action could be blocked by the addition of actidine.
Cao
Y Q
YQ
Laboratory of Reproductive Biology, Academia Sinica, Beijing PRC.
Chen
Y Z
YZ
Zheng
M
M
Zhang
Z Y
ZY
D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D015237:Dinoprost / Q000096:biosynthesis*; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D006801:Humans; D007930:Leucine / Q000378:metabolism; D008213:Lymphocyte Activation; D010455:Peptides / Q000502:physiology*; D011247:Pregnancy; D011255:Pregnancy Maintenance / Q000276:immunology*; D011817:Rabbits; D051381:Rats
D010455:Peptides; D015237:Dinoprost; D007930:Leucine