title: Enhanced antiproliferative activity by metastatic RAW117 lymphoma cells.
abstract: The highly malignant/metastatic murine large cell lymphoma cell line RAW117-H10 forms 100-200 times more liver metastatic tumors than its parental counterpart cell line RAW117-P. RAW117-H10 cells, but not the less malignant/metastatic parental cells, significantly inhibited the mitogen-induced proliferation of normal syngeneic Balb/c and allogeneic ICRC mouse spleen cells. Such an inhibition also occurred when mitomycin-C treated metastatic lymphoma cells were added 24 h after initiation of culture, indicating that no competition with mitogen binding sites on the lymphocytes was necessary for inhibition of proliferation. 'Antiproliferative' cell surface molecules were extracted non-cytolytically from the RAW117-H10 cells using butanol. The butanol extracts from the metastatic RAW117-H10 cells also inhibited the mitogen-induced proliferation and natural killer (NK) cell-mediated cytotoxicity of normal spleen cells. Our results indicate that these 'antiproliferative' cell surface molecules of metastatic murine RAW117-H10 lymphoma cells may have important role(s) in tumor-mediated host immunosuppression.
authors.affiliation: Department of Anatomy, University of Nebraska Medical Center Omaha 68198-6395.
authors.forename: S S
authors.initials: SS
authors.lastname: Joshi
authors.forename: S J
authors.initials: SJ
authors.lastname: O'Connor
authors.forename: D D
authors.initials: DD
authors.lastname: Weisenburger
authors.forename: J G
authors.initials: JG
authors.lastname: Sharp
authors.forename: H M
authors.initials: HM
authors.lastname: Gharpure
authors.forename: K W
authors.initials: KW
authors.lastname: Brunson
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003603:Cytotoxins / Q000494:pharmacology*; D007108:Immune Tolerance; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000187:drug effects; D008223:Lymphoma / Q000737:chemistry* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009362:Neoplasm Metastasis; D013154:Spleen / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D003603:Cytotoxins; D007166:Immunosuppressive Agents