Altered glucose transporter mRNA abundance in a rat model of endotoxic shock.
To better understand molecular mechanisms of glucose transport in shock, we studied glucose transporter isoform mRNA abundance after injection of S. enteritidis endotoxin (40 mg/kg) or saline. Six to 8 hours after injection, endotoxin-treated animals compared to controls became hypoglycemic (44 +/- 6 vs. 111 +/- 4 mg/dl) and lactacidemic (5.9 +/- 0.5 vs. 1.3 +/- 0.1). At such times, tissue RNA was isolated and hybridized to Riboprobes for GLUT1 (erythrocyte), GLUT2 (liver), and GLUT4 (muscle/fat) glucose transporter isoforms and expressed as percent of control. GLUT1 mRNA abundance was increased in fat (660%, p less than .05), soleus muscle (314%, p less than .05), and liver (871%, p less than .001) of endotoxin-treated rats. Soleus muscle GLUT4 mRNA levels were increased (+33%, p less than .02), while liver GLUT2 mRNA levels were markedly decreased (-58%, p less than .01). The overall increase in GLUT1 mRNA abundance accompanied by lowered liver GLUT2 mRNA levels may either cause or reflect profoundly altered glucose transport.
Zeller
W P
WP
Department of Pediatrics, Loyola University Shock and Trauma Institute, Loyola University of Chicago Strich School of Medicine, Maywood, IL 60153.
The
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Sweet
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Goto
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Gottschalk
M E
ME
Hurley
R M
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Filkins
J P
JP
Hofmann
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D000273:Adipose Tissue; D000818:Animals; D001921:Brain / Q000378:metabolism; D004195:Disease Models, Animal; D004912:Erythrocytes / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D009132:Muscles / Q000378:metabolism; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012481:Salmonella Infections, Animal / Q000378:metabolism*; D012477:Salmonella enteritidis; D012772:Shock, Septic / Q000378:metabolism*
D009004:Monosaccharide Transport Proteins; D015347:RNA Probes; D012333:RNA, Messenger