Identification of a unique membrane receptor for adenosine 5',5"'-P1,P4-tetraphosphate.
Adenosine 5',5"'-P1,P4-tetraphosphate (Ap4A) has been implicated as a modulator of cell stress. We have performed binding studies which indicate that membranes from all tissues tested bind tritium-labeled Ap4A. The characteristics of Ap4A binding were determined on brain membrane homogenates after development of an optimized in vitro filter-binding assay. Ap4A binding is specific for adenylated dinucleotides and for the length of the phosphate bridge. A Kd of 0.71 microM for Ap4A was determined.
Hilderman
R H
RH
Department of Biological Sciences, Clemson University, South Carolina 29634-1903.
Martin
M
M
Zimmerman
J K
JK
Pivorun
E B
EB
D000818:Animals; D001921:Brain / Q000378:metabolism; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002412:Cations; D002462:Cell Membrane / Q000378:metabolism*; D015226:Dinucleoside Phosphates / Q000378:metabolism*; D007700:Kinetics; D009928:Organ Specificity; D010542:Peromyscus; D013379:Substrate Specificity
D002352:Carrier Proteins; D002412:Cations; D015226:Dinucleoside Phosphates; C020733:diadenosine tetraphosphate