title: Leucine metabolism during fasting and exercise.
abstract: Whole body leucine kinetics were examined in seven healthy young men while in a 14-h postabsorptive state (PAS) and after a 3.5-day fast (FS). Subjects received a primed constant intravenous infusion of L-[1-13C]leucine while resting for 3 h and then while exercising on a cycle ergometer at 45% maximal O2 uptake to exhaustion. Blood samples drawn during isotopic steady state were analyzed for 13C enrichment of leucine and alpha-ketoisocaproic acid, and expired gas samples were analyzed for 13CO2. Resting leucine flux was higher in the FS, and there was a slight increase in leucine oxidation. During exercise, leucine flux did not differ between PAS and FS but leucine oxidation rose markedly. In the FS, leucine oxidation was 25 +/- 7 (SD) mumol.kg-1.h-1 at rest and rose to 75 +/- 21 mumol.kg-1.h-1 during exercise; in the PAS, oxidation was 20 +/- 5 mumol.kg-1.h-1 at rest and 52 +/- 17 mumol.kg-1.h-1 during exercise. These data indicate that the high rate of leucine oxidation previously found during exercise was increased further by a 3.5-day fast.
authors.affiliation: US Army Research Institute of Environmental Medicine, Natick 01760-5007.
authors.forename: J
authors.initials: J
authors.lastname: Knapik
authors.forename: C
authors.initials: C
authors.lastname: Meredith
authors.forename: B
authors.initials: B
authors.lastname: Jones
authors.forename: R
authors.initials: R
authors.lastname: Fielding
authors.forename: V
authors.initials: V
authors.lastname: Young
authors.forename: W
authors.initials: W
authors.lastname: Evans
mesh_terms: D000328:Adult; D004734:Energy Metabolism; D015444:Exercise / Q000502:physiology*; D005215:Fasting; D006801:Humans; D007700:Kinetics; D007930:Leucine / Q000378:metabolism*; D008297:Male; D010084:Oxidation-Reduction; D011506:Proteins / Q000378:metabolism
chemical_list: D011506:Proteins; D007930:Leucine