Thermospray and particle beam liquid chromatographic-mass spectrometric analysis of coumarin anticoagulants.
Positive ion mass spectra were obtained from several coumarin oral anticoagulants (phenprocoumon, warfarin, acenocoumarol and dicoumarol) and derivatives by liquid chromatography-thermospray mass spectrometry (LC-TSP-MS) and liquid chromatography-electron impact mass spectrometry (LC-EI-MS) to assess the use of LC-MS methods for the determination of these compounds in biological materials. LC-TSP mass spectra showed a single [M + 1]+ ion with no fragmentation; LC-EI mass spectra showed fragment ions which were similar in mass and relative intensities to those obtained by conventional EI-MS. These data should serve as a basis for the development of LC-MS methods for the qualitative and quantitative analysis of coumarin anticoagulants in biological samples. LC-TSP-MS was applied to the determination of phenprocoumon in a plasma extract from an anticoagulated patient.
de Vries
J X
JX
Abteilung für Klinische Pharmakologie, Medizinische Klinik, Universität Heidelberg, F.R.G.
Kymber
K A
KA
D000925:Anticoagulants / Q000032:analysis* / Q000097:blood / Q000652:urine; D002853:Chromatography, Liquid; D003374:Coumarins / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D013058:Mass Spectrometry; D010644:Phenprocoumon / Q000097:blood
D000925:Anticoagulants; D003374:Coumarins; D010644:Phenprocoumon