title: Apical and basolateral conductance in cultured A6 cells.
abstract: Confluent monolayers of the cultured renal distal tubule cell line (A6) were impaled with microelectrodes under short-circuit conditions. Specific membrane conductances were calculated from equivalent circuit equations. Transport properties of the apical and basolateral membranes were investigated during control conditions and short-term increases in basolateral potassium concentration [K+] from 2.5 to 20 mmol/l, with or without 0.5 mmol/l Ba2+ at the basolateral side. As in most other epithelia, the apical membrane represents the major resistive barrier. Transcellular, apical and basolateral membrane conductances (gc, go and gi respectively), obtained from 22 acceptable microelectrode studies, averaged 61, 80 and 292 microS/cm2, respectively. There was a highly significant correlation between short-circuit current (Isc) and go, whereas gi was unrelated to Isc. The Isc, which averaged 4.1 microA/cm2, was almost completely blocked by amiloride. This was associated with fast hyperpolarization; the intracellular potential (Vsc) increased from -69 to -83 mV and the fractional apical resistance rose to nearly 100%. Using the values of Vsc during amiloride at normal and high [K+], an apparent transference number for K+ at the basolateral membrane of 0.72 can be calculated. This value corresponds with the decrease in gi to about 25% of the control values after blocking the K+ channels with Ba2+. The nature of the remaining conductance is presently unclear. The cellular current decreased during high [K+] and Ba2+, in part resulting from reduction of the electrochemical gradient for apical Na+ uptake due to the depolarization.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Département de Physiologie, Université Catholique de Louvain, Bruxelles, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Granitzer
authors.forename: T
authors.initials: T
authors.lastname: Leal
authors.forename: W
authors.initials: W
authors.lastname: Nagel
authors.forename: J
authors.initials: J
authors.lastname: Crabbe
mesh_terms: D000818:Animals; D001464:Barium / Q000494:pharmacology; D001692:Biological Transport; D002460:Cell Line; D004553:Electric Conductivity; D007686:Kidney Tubules, Distal / Q000502:physiology*; D008564:Membrane Potentials / Q000502:physiology; D008839:Microelectrodes; D011188:Potassium / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: D001464:Barium; D011188:Potassium