Monoclonal antibodies against a preadipose cell line (MC3T3-G2/PA6) which can support hemopoiesis.
Monoclonal antibodies against a mouse preadipose cell line (MC3T3-G2/PA6:PA6), which can support hemopoiesis by direct cell-to-cell interaction, were produced and characterized. The antibodies react with PA6 but not PA6-M (a mutant cell line) which has the capacity neither to contact with hemopoietic stem cells (HSCs) nor to support hemopoiesis. Endosteal cells in the bone marrow show positive staining to these antibodies. They inhibit pseudoemperipolesis of PA6 to HSCs, resulting in a significant decrease in hemopoietic cell number. These findings suggest that the monoclonal antibodies bind to the stromal cell receptors for HSCs and block the binding of HSCs to stromal cells leading to suppression of hemopoiesis.
Izumi-Hisha
H
H
Department of Pathology, Kansai Medical University, Osaka, Japan.
Than
S
S
Ogata
H
H
Inaba
M
M
Ikehara
S
S
Kawai
M
M
D000273:Adipose Tissue / Q000276:immunology* / Q000502:physiology; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001665:Binding Sites; D002450:Cell Communication / Q000276:immunology / Q000502:physiology; D002460:Cell Line; D006410:Hematopoiesis / Q000276:immunology* / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology / Q000502:physiology; D006825:Hybridomas / Q000276:immunology; D007108:Immune Tolerance; D051379:Mice; D051381:Rats; D013234:Stem Cells / Q000276:immunology / Q000502:physiology
D000911:Antibodies, Monoclonal