title: Contribution of rabbit leukocyte defensins to the host response in experimental syphilis.
abstract: In the companion paper (L. A. Borenstein, M. E. Selsted, R. I. Lehrer, and J. N. Miller, Infect. Immun. 59:1359-1367, 1991), we report that rabbit alveolar macrophage and neutrophil derived defensins possess antimicrobial activity against Treponema pallidum subsp. pallidum, the etiologic agent of syphilis. In this study, antisera specific for NP-1 and NP-2 (defensins present in certain macrophages and polymorphonuclear leukocytes) and NP-5 (a defensin produced only in neutrophils) were used to detect these peptides by immunoperoxidase staining in testicular lesions from infected rabbits. Profound amounts of cell-free and cell-associated defensins were detected in the tunica albuginea and interstitial spaces during the first 24 h of infection. The presence of defensins was transient and almost undetectable by day 4. Interstitial defensins were detected again at day 10 and increased through day 16, at which time lesion healing was evident by hematoxylin and eosin staining. The appearance and increase in detectable defensins between days 10 and 16 of infection correlated with a reduction in numbers and disappearance of T. pallidum, as demonstrated by using silver staining. The extent and pattern of immunostaining for NP-1 and NP-2 corresponded with immunostaining for NP-5 and identified neutrophils as the cellular source of the defensins. These findings indicate that defensins may contribute to the control of local T. pallidum infection and suggest a role for acute inflammatory processes in the resolution of early experimental syphilis.
authors.affiliation: Department of Microbiology and Immunology, University of California, Los Angeles 90024.
authors.forename: L A
authors.initials: LA
authors.lastname: Borenstein
authors.forename: T
authors.initials: T
authors.lastname: Ganz
authors.forename: S
authors.initials: S
authors.lastname: Sell
authors.forename: R I
authors.initials: RI
authors.lastname: Lehrer
authors.forename: J N
authors.initials: JN
authors.lastname: Miller
mesh_terms: D000818:Animals; D001770:Blood Bactericidal Activity; D001798:Blood Proteins / Q000032:analysis / Q000502:physiology*; D023082:Defensins; D007106:Immune Sera / Q000276:immunology; D008297:Male; D009504:Neutrophils / Q000276:immunology*; D011817:Rabbits; D013587:Syphilis / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D013737:Testis / Q000473:pathology; D014210:Treponema pallidum / Q000302:isolation & purification
chemical_list: D001798:Blood Proteins; D023082:Defensins; D007106:Immune Sera