title: Influence of nutrition and bovine growth hormone (GH) on hepatic GH binding, insulin-like growth factor-I and growth of lambs.
abstract: The effect on young lambs of 0.25 mg recombinant bovine GH (bGH)/kg per day on plasma concentrations of insulin-like growth factor-I (IGF-I), glucose, specific hepatic GH binding and body composition changes was examined at two levels of nutrition (lucerne pellets; 3 and 1.7% of body weight/day). Lambs on low levels of nutrition had low plasma IGF-I (P less than 0.001). Plasma concentrations of IGF-I were increased by bGH treatment at both levels of nutrition, with the high nutrition group showing the greatest IGF-I response after 3 and 40 days of bGH treatment. Plasma glucose, after 40 days, was higher overall (P less than 0.05) in lambs on high nutrition. bGH treatment increased plasma glucose, with the response being greater in the well-fed lambs. Specific binding of GH to liver membranes was highest in lambs on high nutrition and on bGH treatment; no significant interaction between nutrition and bGH treatment was detected, indicating that specific binding of GH was increased proportionally by bGH at both nutritional levels. The major change in body composition was the reduced level of fatness in lambs treated with bGH. There was no significant effect of bGH on body weight although bGH treatment tended to increase weight gain of well-fed lambs and decreased weight loss of poorly nourished lambs. The results show that, although there was a significant (P less than 0.05) bGH/nutrition interaction for IGF-I there was no such interaction for body weight/components or specific GH binding to the liver. The results indicate that an increase in plasma IGF-I does not necessarily result in increases in growth or changes in carcass composition.
authors.affiliation: Ruakura Agricultural Centre, Private Bag, Hamilton, New Zealand.
authors.forename: J J
authors.initials: JJ
authors.lastname: Bass
authors.forename: J M
authors.initials: JM
authors.lastname: Oldham
authors.forename: S C
authors.initials: SC
authors.lastname: Hodgkinson
authors.forename: P J
authors.initials: PJ
authors.lastname: Fowke
authors.forename: H
authors.initials: H
authors.lastname: Sauerwein
authors.forename: P
authors.initials: P
authors.lastname: Molan
authors.forename: B H
authors.initials: BH
authors.lastname: Breier
authors.forename: P D
authors.initials: PD
authors.lastname: Gluckman
mesh_terms: D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001823:Body Composition / Q000502:physiology; D006128:Growth / Q000187:drug effects*; D013006:Growth Hormone / Q000378:metabolism / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D009919:Orchiectomy; D011994:Recombinant Proteins / Q000494:pharmacology; D012756:Sheep / Q000378:metabolism*
chemical_list: D001786:Blood Glucose; D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone