Variability of polyadenylation sites in mRNAs from human fetal liver.
cDNA libraries of human fetal liver were constructed in pBR322 and lambda gt10 vectors. The libraries were screened for liver-specific clones by differential hybridization. This procedure revealed 25 and 32 liver-specific clones in plasmid and phage libraries, respectively. The majority of these clones were represented with serum albumin, fetal G gamma-globin and A gamma-globin cDNA inserts. Three types of 3'-non-coding region were found in 5 sequenced albumin cDNAs. In one type mRNA the distance between the AATAAA signal and polyadenylation site was 15 nucleotides, in 2 other types this distance was 10 and 6 nucleotides. The polyadenylation site in the G gamma-globin cDNA was located 2 nucleotides further from AATAAA signal, while in the A gamma-globin cDNA it was 2 nucleotides closer to the signal as compared with the results published previously.
Dmitrenko
V V
VV
Department of Biosynthesis of Nucleic Acids, Ukr. SSR Academy of Sciences, Kiev.
Kavsan
V M
VM
D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry; D005333:Fetus; D015698:Genomic Library; D005914:Globins / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000737:chemistry* / Q000196:embryology / Q000378:metabolism; D008969:Molecular Sequence Data; D011061:Poly A / Q000235:genetics*; D012333:RNA, Messenger / Q000737:chemistry*; D012709:Serum Albumin / Q000235:genetics*
D012333:RNA, Messenger; D012709:Serum Albumin; D011061:Poly A; D005914:Globins; D004247:DNA