Intracellular localization of a chalcogenapyrylium dye probed by spectroscopy and sites of photodamage.
Site(s) of intracellular localization of a photosensitizing chalcogenapyrylium dye were assessed using murine leukemia cells in culture. While the dye exhibited substantial dark toxicity, additional damage was elicited by irradiation. The fluorescence emission spectrum of intracellular dye suggests an initial moderately hydrophobic site of localization (dielectric constant approx. 20). This might represent a membrane interface. But longer incubations led to alterations in both fluorescence emission and absorbance spectra, indicative of both dye migration to a more hydrophilic cellular site and dye biotransformations. Dye-induced cytotoxicity, in either light or dark, was associated with mitochondrial, rather than membrane damage.
Kessel
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Department of Pharmacology, Wayne State University School of Medicine, Detroit, MI 48201.
D000818:Animals; D001555:Benzene Derivatives / Q000378:metabolism / Q000494:pharmacology*; D001692:Biological Transport; D002470:Cell Survival / Q000187:drug effects*; D007939:Leukemia L1210; D008027:Light; D051379:Mice; D016566:Organoselenium Compounds; D011838:Radiation-Sensitizing Agents / Q000378:metabolism* / Q000494:pharmacology; D012643:Selenium / Q000378:metabolism / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence / Q000379:methods
D001555:Benzene Derivatives; D016566:Organoselenium Compounds; D011838:Radiation-Sensitizing Agents; C060012:chalogenapyrylium dye 8b; D012643:Selenium