title: DNA methylation and expression of the genes coding for lactate dehydrogenases A and C during rodent spermatogenesis.
abstract: The testis chromatin undergoes profound structural alterations and functional changes during spermatogenesis. Changes in DNA methylation have been correlated with gene expression in a number of systems, but the relationship between methylation and gene expression for testicular genes is unclear. To address this question, DNA methylation patterns and mRNA expression for a somatic form of lactate dehydrogenase (LDH), LDH-A, were compared with those of the testis-specific form, LDH-C, in preparations from testes of prepubertal and sexually mature mice, from isolated testicular cells, and from somatic tissues. At specific sites, LDH-A was less methylated in adult testis than in spleen DNA; the decreased methylation in the testicular DNA occurred as early as type A spermatogonia. In contrast, DNA methylation patterns for LDH-C did not differ between spleen and testis DNAs. In Northern blots, the levels of LDH-A transcripts were low in total testis RNA obtained from 6-12-day-old mice, and in type A and B spermatogonia from 8-day-old mice. LDH-A mRNA levels increased gradually in testes from 16-45-day-old mice. LDH-C transcripts were first detectable in the testes of 12-day-old mice and increased as spermatogenesis proceeded. Both LDH-A and LDH-C mRNA levels were low in preleptotene spermatocytes and leptotene/zygotene spermatocytes and increased substantially in pachytene spermatocytes and round spermatids. Reduced levels of LDH-A and LDH-C mRNAs were found in the residual bodies/cytoplasts fraction.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Biology, Tufts University, Medford, Massachusetts 02155.
authors.forename: A A
authors.initials: AA
authors.lastname: Alcivar
authors.forename: J M
authors.initials: JM
authors.lastname: Trasler
authors.forename: L E
authors.initials: LE
authors.lastname: Hake
authors.forename: K
authors.initials: K
authors.lastname: Salehi-Ashtiani
authors.forename: E
authors.initials: E
authors.lastname: Goldberg
authors.forename: N B
authors.initials: NB
authors.lastname: Hecht
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D008297:Male; D008745:Methylation; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013091:Spermatogenesis / Q000235:genetics* / Q000502:physiology; D013737:Testis / Q000166:cytology / Q000201:enzymology / Q000254:growth & development
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D004247:DNA; D007770:L-Lactate Dehydrogenase