ATP-dependent transport for glucuronides in canalicular plasma membrane vesicles.
Using rat liver canalicular plasma membrane vesicles, it has been verified that the transport of p-nitrophenyl glucuronide (NPG) across membranes is an ATP-dependent process; the apparent Km for NPG was 20 microM. S-(2,4-dinitrophenyl)-glutathione (DNP-SG) inhibited NPG uptake dose-dependently, and NPG or testosterone glucuronide did ATP-dependent DNP-SG uptake similarly. These results suggest that transport of glucuronide is mediated by an ATP-dependent glutathione S-conjugate carrier.
Kobayashi
K
K
Institute of Scientific and Industrial Research, Osaka University, Japan.
Komatsu
S
S
Nishi
T
T
Hara
H
H
Hayashi
K
K
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D005965:Glucuronates / Q000378:metabolism*; D005978:Glutathione / Q000031:analogs & derivatives* / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D008958:Models, Molecular; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000031:analogs & derivatives* / Q000378:metabolism
D005965:Glucuronates; C002730:4-nitrophenylglucuronide; C015529:testosterone glucuronate; C030190:S-(2,4-dinitrophenyl)glutathione; D013739:Testosterone; D000255:Adenosine Triphosphate; D005978:Glutathione