title: Effect of fat pre-feeding on bile flow and composition in the rat.
abstract: Studies were conducted in rats to determine if the increase in lymph triacylglycerol output on pre-feeding a 20% glyceryltrioleate diet (Mansbach, C.M., II and Arnold, A. (1986) Am. J. Physiol. 251, G263-269) was due to an increase in phosphatidylcholine output into bile. Rats who were fed chow or pre-fed the 20% fat diet were equipped with biliary and duodenal cannulas and infused with glucose-saline while bile was collected hourly. The next day a taurocholate-glyceryltrioleate infusion was given and bile collected for 5 h. Bile flow, bile acid, phosphatidylcholine and cholesterol output were greater in the chow fed group than controls during the 6 h of the glucose saline period. Outputs were low overnight. During the taurocholate-glyceryltrioleate infusion, bile flow, bile acid, phosphatidylcholine and cholesterol output were all greater in the fat pre-fed group than the chow fed controls. We conclude that fat pre-feeding profoundly influences biliary composition and flow. The 2-fold increase in biliary phosphatidylcholine output during duodenal lipid infusion offers a potential explanation for the increased delivery of triacylglycerol into the lymph in rats on a similar fat pre-feeding program.
authors.affiliation: Department of Medicine, University of Tennessee, Memphis 38163.
authors.forename: R
authors.initials: R
authors.lastname: Knox
authors.forename: I
authors.initials: I
authors.lastname: Stein
authors.forename: D
authors.initials: D
authors.lastname: Levinson
authors.forename: P
authors.initials: P
authors.lastname: Tso
authors.forename: C M
authors.initials: CM
authors.lastname: Mansbach
mesh_terms: D000818:Animals; D001646:Bile / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000032:analysis*; D002784:Cholesterol / Q000378:metabolism; D004041:Dietary Fats / Q000494:pharmacology*; D008196:Lymph / Q000502:physiology; D010713:Phosphatidylcholines / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013656:Taurocholic Acid / Q000494:pharmacology*; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism*; D014304:Triolein / Q000494:pharmacology*
chemical_list: D001647:Bile Acids and Salts; D004041:Dietary Fats; D010713:Phosphatidylcholines; D014280:Triglycerides; D014304:Triolein; D013656:Taurocholic Acid; D002784:Cholesterol