title: Pericellular glycoconjugates of cultured fibroblasts from control and cystic fibrosis patients.
abstract: 1. Labeled glycoconjugates released by trypsin from cell surfaces of control and cystic fibrosis (CF) skin fibroblasts were purified and fractionated by column chromatography on Sephadex G-50 and Concanavalin A Sepharose. Based on chemical analysis and specific enzymatic digestions: (1) Glycoconjugates were characterized as O-linked glycopeptides consisting predominantly of glycosaminoglycan type and N-linked glycopeptides with glycans of complex type. Their relative proportions were similar between the two groups. (2) The N-linked glycopeptides exhibited an increased molar ratio of fucose to galactose in CF fibroblasts. (3) When pericellular glycoconjugates were metabolically labeled with [14C]glucosamine and [3H]fucose, incorporation and degradation kinetics were similar between the two groups.
authors.affiliation: Laboratoire de Biochimie, I.N.S.E.R.M.-U. 181, Faculté de Médecine Saint-Antoine, Paris, France.
authors.forename: F
authors.initials: F
authors.lastname: Bertrand
authors.forename: B
authors.initials: B
authors.lastname: Hermelin
authors.forename: A
authors.initials: A
authors.lastname: Paul
authors.forename: J
authors.initials: J
authors.lastname: Picard
mesh_terms: D002241:Carbohydrates / Q000032:analysis; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003550:Cystic Fibrosis / Q000378:metabolism*; D005347:Fibroblasts; D005643:Fucose / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D006001:Glycoconjugates / Q000032:analysis* / Q000302:isolation & purification; D006020:Glycopeptides / Q000302:isolation & purification; D006025:Glycosaminoglycans / Q000032:analysis / Q000302:isolation & purification; D006031:Glycosylation; D006801:Humans; D007700:Kinetics; D014357:Trypsin / Q000378:metabolism
chemical_list: D002241:Carbohydrates; D006001:Glycoconjugates; D006020:Glycopeptides; D006025:Glycosaminoglycans; D005643:Fucose; D014357:Trypsin; D005944:Glucosamine