title: In vitro mutagenesis and plasmid shuffling: from cloned gene to mutant yeast.
authors.forename: R S
authors.initials: RS
authors.lastname: Sikorski
authors.forename: J D
authors.initials: JD
authors.lastname: Boeke
mesh_terms: D000483:Alleles; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005800:Genes, Fungal; D005822:Genetic Vectors; D008957:Models, Genetic; D016296:Mutagenesis; D010957:Plasmids; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development