title: Loss of a tumor suppressor gene function is correlated with downregulation of chondrocyte-specific collagen expression in Syrian hamster embryo cells.
abstract: We previously described the isolation of closely related, preneoplastic Syrian hamster cell lines that have retained (supB+) or lost (supB-) the ability to suppress the anchorage-independent growth and tumorigenicity of a sarcoma cell line (BP6T) in cell hybrids. In this report, we have used differential cDNA screening to clone several genes that are expressed in supB+ cells and downregulated in supB- cells. The nontumorigenic supB+ and supB- variants are advantageous for differential cDNA cloning because multiple independent cell lines differing in their tumor suppressor activity have been isolated. Differentially expressed cDNAs were isolated and placed into one of four groups based on DNA cross-hybridization. Representative cDNAs from Groups I and II, which were expressed at relatively high levels in two independently derived supB+ cell lines (DES4 and 10W) and downregulated in the supB- and tumor cell lines, were sequenced. The DNA and predicted amino acid sequences of these genes were found to be highly homologous to the chondrocyte-specific collagens type II and type IX. In contrast to the chondrocyte-specific collagens, another collagen isoform, collagen type I, was expressed at similar levels in both supB+ and supB- cells. These results suggest that carcinogen-induced immortalization selected for chondrocyte-like cell lines from the mixed embryo cell population. As these cells progressed toward tumorigenicity, the ability to express the chondrocyte differentiation markers was lost concomitantly with the ability to suppress the tumorigenicity of the BP6T sarcoma cell line. These results are consistent with the hypothesis that the supB+ tumor suppressor gene is involved in the regulation of differentiation. The identification of genes regulated by this suppressor gene may aid in its isolation.
authors.affiliation: Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
authors.forename: P E
authors.initials: PE
authors.lastname: Cizdziel
authors.forename: J
authors.initials: J
authors.lastname: Hosoi
authors.forename: J C
authors.initials: JC
authors.lastname: Montgomery
authors.forename: R W
authors.initials: RW
authors.lastname: Wiseman
authors.forename: J C
authors.initials: JC
authors.lastname: Barrett
mesh_terms: D000818:Animals; D001483:Base Sequence; D002356:Cartilage / Q000166:cytology*; D003094:Collagen / Q000235:genetics*; D006224:Cricetinae; D004273:DNA, Neoplasm / Q000737:chemistry*; D015536:Down-Regulation; D005354:Fibrosarcoma / Q000235:genetics*; D015723:Gene Library; D016153:Genes, Suppressor / Q000235:genetics*; D008647:Mesocricetus; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics*; D013045:Species Specificity; D014407:Tumor Cells, Cultured
chemical_list: D004273:DNA, Neoplasm; D012333:RNA, Messenger; D003094:Collagen