Linkage of the erythroid transcription factor gene (Gf-1) to the proximal region of the X chromosome of mice.
We have used a cDNA probe for mouse Gf-1 gene that encodes the erythroid cell transcription factor to identify genetic variation in genomic DNA between Mus species. The segregation of Gf-1 DNA variation was analyzed in Mus species crosses that have been previously typed for the segregation of more than 30 genes spanning 80 cM of the mouse X chromosome from the centromere to the border of the X-Y pairing region. We identified a single X chromosome locus in the mouse, Gf-1, and an analysis of recombinants from 203 backcross progeny mapped Gf-1 to the proximal portion of the chromosome, coincident with the Cybb locus and proximal to Otc gene locus. A gene order of centromere, DXWas70, Cybb/Gf-1, Otc, Timp was established for the mouse X chromosome, which is in agreement with the map position observed on the human X chromosome.
Chapman
V M
VM
Molecular and Cellular Biology Department, Roswell Park Cancer Institute, Buffalo, New York 14263.
Stephenson
D A
DA
Mullins
L J
LJ
Keitz
B T
BT
Disteche
C
C
Orkin
S H
SH
D000818:Animals; D015139:Blotting, Southern; D003433:Crosses, Genetic; D004268:DNA-Binding Proteins / Q000235:genetics*; D050981:Erythroid-Specific DNA-Binding Factors; D005260:Female; D008040:Genetic Linkage; D014644:Genetic Variation; D008297:Male; D051379:Mice; D011995:Recombination, Genetic; D014157:Transcription Factors / Q000235:genetics*; D014960:X Chromosome
D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D014157:Transcription Factors