Gene and precursor structures of human C-type natriuretic peptide.
We have isolated the gene for human C-type natriuretic peptide (CNP) from a human genomic library using a cloned porcine CNP genomic DNA fragment as probe. Human CNP gene consists of at least two coding blocks and an intron, and encodes a 126-residue CNP precursor (human prepro-CNP). From a comparison of the amino acid sequences of porcine and rat prepro-CNPs, human prepro-CNP is found to be processed to generate 22-and 53-residue peptides (human CNP-22 and human CNP-53, respectively) as major endogenous CNPs in human. Interestingly, human CNP-53 has two amino acid substitutions as compared to the porcine and rat CNP-53s, whereas human CNP-22 is identical to the porcine and rat CNP-22s. Intravenous injection of human CNP-53 into anesthetized rats induces diuretic-natriuretic and hypotensive activities with same potencies as porcine CNP-53 does, although these activities were considerably lower (about 1/100) than those of human alpha-ANP.
Tawaragi
Y
Y
Suntory Institute for Biomedical Research, Osaka, Japan.
Fuchimura
K
K
Tanaka
S
S
Minamino
N
N
Kangawa
K
K
Matsuo
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001794:Blood Pressure / Q000187:drug effects; D003001:Cloning, Molecular; D004231:Diuresis / Q000187:drug effects; D005796:Genes; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D020098:Natriuretic Peptide, C-Type; D009419:Nerve Tissue Proteins / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics; D051381:Rats; D015183:Restriction Mapping; D013552:Swine
D009419:Nerve Tissue Proteins; D011498:Protein Precursors; D020098:Natriuretic Peptide, C-Type