title: Adherence of coagulase-negative staphylococci onto polyethylene catheters in vitro and in vivo: a study on the influence of various plasma proteins.
abstract: Bacterial adherence on PE catheters inserted into the carotid artery of rabbits was assessed at 5, 30, and 240 min after injection with bacteria of five coagulase-negative staphylococci (CN Staph). These studies revealed that CN Staph adhere onto PE catheters 5 min after injection with bacteria. At 240 min after injection with bacteria almost all catheters were sterile, indicating that initially adherent bacteria on PE catheters disappear with time. EM studies revealed high numbers of platelets and leukocytes and many fibrin deposits on the surface of the PE catheter. In addition, the adherence of the five test strains on PE catheters was determined in vitro. In these in vitro studies the bacteria and catheters were pretreated with either fibrinogen, fibronectin, albumin or citrated whole plasma or the liquid adherence medium was supplemented with these proteins or the adherence assay was done in citrated whole plasma. The presence of proteins in the adherence medium or pretreatment of the catheter or bacteria with either fibronectin, albumin or whole citrated plasma markedly inhibited bacterial adherence. In contrast, pretreatment of bacteria or both bacteria and catheters with fibrinogen enhanced bacterial adherence suggesting the presence of ligands for fibrinogen on the cell surface of CN Staph.
authors.affiliation: Department of Medical Microbiology, University of Amsterdam, The Netherlands.
authors.forename: P
authors.initials: P
authors.lastname: Brokke
authors.forename: J
authors.initials: J
authors.lastname: Dankert
authors.forename: J
authors.initials: J
authors.lastname: Carballo
authors.forename: J
authors.initials: J
authors.lastname: Feijen
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001424:Bacterial Infections / Q000209:etiology; D001798:Blood Proteins; D002404:Catheterization; D003030:Coagulase / Q000032:analysis; D005547:Foreign Bodies / Q000150:complications; D006801:Humans; D007958:Leukocyte Count; D008855:Microscopy, Electron, Scanning; D010976:Platelet Count; D011095:Polyethylenes; D011817:Rabbits; D013210:Staphylococcus / Q000201:enzymology
chemical_list: D001798:Blood Proteins; D003030:Coagulase; D011095:Polyethylenes