Role of the extracellular matrix on the growth and differentiated phenotype of murine colonic adenocarcinoma cells in vitro.
The growth and differentiation characteristics of MAC 15 murine adenocarcinoma cells, derived from routine passage in vivo for growth in vitro on a plastic substrate (MAC15j cells), were compared under conditions in which the cells were seeded onto a substrate of type-I collagen which was either attached to plastic or was released to float free in medium. Cells grown on a plastic substrate consisted of a heterogeneous, largely anaplastic population with a putative enterocytic morphology but with no evidence of junctional complexes or cell polarity typical of an epithelial phenotype. MAC 15j cells from cultures grown on a plastic substrate reestablished a moderate to well-defined degree of differentiation when transplanted back into NMRI mice. When MAC 15j cells were seeded from plastic onto type-I collagen, either attached to plastic or free-floating, tight junctional complexes were formed and the cells began to attain a more recognizable, columnar and polarised epithelial morphology. Cells grown on a type-I collagen gel which was free-floating showed a selective expression of alkaline phosphatase at the apical surfaces of approximately 10% of the cells. This expression was detectable by electron microscope histochemistry but could not be detected biochemically. Treatment of MAC 15j cells grown on a released collagen matrix with tetramethyl-urea (20mM) accelerated the expression of alkaline phosphatase activity at the apical surface as detected by microscopy.
Walling
J M
JM
Cancer Research Campaign Experimental Chemotherapy Research Group, Aston University, Birmingham, UK.
Blackmore
M
M
Hickman
J A
JA
Townsend
K M
KM
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002454:Cell Differentiation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D003114:Colony-Forming Units Assay; D004126:Dimethylformamide / Q000494:pharmacology; D005109:Extracellular Matrix / Q000502:physiology*; D066298:In Vitro Techniques; D008782:Methylurea Compounds / Q000494:pharmacology; D051379:Mice; D008854:Microscopy, Electron
D002087:Butyrates; D008782:Methylurea Compounds; D020148:Butyric Acid; C004168:1,1,3,3-tetramethylurea; D004126:Dimethylformamide; D000469:Alkaline Phosphatase