Involvement of reactive oxygen species in post-ischaemic flap necrosis and its prevention by antioxidants.
In attempts to substantiate the possible participation of reactive oxygen species, and the significance of the xanthine oxidase system in both post-ischaemic reperfusion necrosis of the island flap and distal necrosis of the pedicle flap, and to develop new pharmacological measures for salvaging flap necrosis, a series of experiments were made using an island flap model and a random-pattern flap model in rats. The results were as follows: (1) Epoxysuccinyl derivative (E-64c), allopurinol and L-SOD salvaged post-ischaemic reperfusion necrosis of the island flaps; (2) E-64c and allopurinol did not salvage anticipated necrosis of the distal region of random flaps but L-SOD did; (3) tissue SOD activity did not reflect the fate of the island flap, but did of the distal region of the random flap. These results demonstrated a possible involvement of ROS in both post-ischaemic necrosis of island flaps and distal necrosis of random flaps. However, xanthine oxidase was significant in producing ROS only in the former.
Suzuki
S
S
Department of Plastic Surgery, Faculty of Medicine, Kyoto University, Japan.
Yoshioka
N
N
Isshiki
N
N
Hamanaka
H
H
Miyachi
Y
Y
D000493:Allopurinol / Q000627:therapeutic use; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D015853:Cysteine Proteinase Inhibitors / Q000627:therapeutic use; D007930:Leucine / Q000031:analogs & derivatives / Q000627:therapeutic use; D008297:Male; D009336:Necrosis / Q000517:prevention & control; D010100:Oxygen; D051381:Rats; D011919:Rats, Inbred Strains; D015427:Reperfusion Injury / Q000517:prevention & control*; D012867:Skin / Q000201:enzymology; D013482:Superoxide Dismutase / Q000627:therapeutic use; D013524:Surgical Flaps; D014969:Xanthine Oxidase / Q000378:metabolism
D000975:Antioxidants; D015853:Cysteine Proteinase Inhibitors; D000493:Allopurinol; C032425:N-(N-(3-carboxyoxirane-2-carbonyl)leucyl)isoamylamine; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D007930:Leucine; D010100:Oxygen