Metabolism of oxidized linoleic acid: distribution of activity for the enzymatic oxidation of 13-hydroxyoctadecadienoic acid to 13-oxooctadecadienoic acid in rat tissues.
Oxidation products of linoleic acid, including hydroperoxy- and hydroxyoctadecadienoic acids have been shown to possess biological activities in a number of different systems. In this work we describe an enzymatic activity which catalyzes the conversion of 13-hydroxyoctadecadienoic acid to a 2,4-dienone product, 13-oxooctadecadienoic acid. The enzyme activity is widely distributed, with the highest activity in the colon and the liver. The distribution of activity among various tissues is distinct from other dehydrogenases known to use oxygenated unsaturated fatty acids as substrates. This enzyme may play a key role in the metabolism of 13-hydroxyoctadecadienoic acid in epithelial tissues.
Bull
A W
AW
Department of Chemistry, Oakland University, Rochester, MI 48309.
Earles
S M
SM
Bronstein
J C
JC
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000818:Animals; D003106:Colon / Q000201:enzymology; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism*; D008042:Linolenic Acids / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008297:Male; D009928:Organ Specificity; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000201:enzymology
D008041:Linoleic Acids; D008042:Linolenic Acids; C064441:13-oxo-9,11-octadecadienoic acid; C024348:13-hydroxy-9,11-octadecadienoic acid; D019787:Linoleic Acid; D000429:Alcohol Oxidoreductases; C067701:13-hydroxyoctadecadienoic acid dehydrogenase