[HLA B27 phenotyping using flow cytometry].
Results of typing for HLA B27 antigens of 106 patients in whole blood with a FITC monoclonal anti HLA B27 antibody and analysis by flow cytometry are compared to the results obtained by the classical microlymphocytoxicity test. By flow cytometry analysis, there are not false negative result, but, because of cross reactions of the used monoclonal antibody with other specificities of the CREG B7 group, false positive result may be encountered. The flow cytometry analysis for typing HLA B27 antigens using a monoclonal antibody can be a good screening technique: so it's rapid and moreover the negative results can be accepted. The positive results must be confirmed.
Kravtzoff
R
R
Canepa
S
S
Boulanger
M D
MD
Babault
C
C
Chassaigne
M
M
D000911:Antibodies, Monoclonal; D003165:Complement System Proteins / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D005434:Flow Cytometry; D015796:HLA-B27 Antigen / Q000032:analysis*; D006801:Humans; D016130:Immunophenotyping; D011237:Predictive Value of Tests
D000911:Antibodies, Monoclonal; D015796:HLA-B27 Antigen; D003165:Complement System Proteins