A novel hemidesmosomal plaque component: tissue distribution and incorporation into assembling hemidesmosomes in an in vitro model.
The hemidesmosome and its associated structures, such as anchoring fibrils, form a complex structure, the polypeptide composition of which has only recently begun to be elucidated. We describe the characterization of a monoclonal antibody, mAb6A5, directed against a 200-kDa polypeptide found in the cytoplasmic-most area of the hemidesmosomal plaque. This 200-kDa polypeptide is immunologically distinct from the 180- and 230-kDa hemidesmosomal plaque components recognized by bullous pemphigoid (BP) autoantibodies. mAb6A5 recognizes hemidesmosomes of stratified squamous epithelia in a number of species, including human tissue. mAb6A5 also recognizes pseudo-stratified epithelium, but not simple or transitional epithelia. During de novo hemidesmosome assembly in an in vitro model of epithelial wound healing, the 200-kDa polypeptide is in most instances deposited at the epithelial-stromal interface after plaque components recognized by BP autoantibodies, but before the collagen type VII component of anchoring fibrils. We discuss possible mechanisms of hemidesmosomal plaque assembly.
Kurpakus
M A
MA
Department of Cell, Molecular and Structural Biology, Northwestern University Medical School, Chicago, Illinois 60611.
Jones
J C
JC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology; D001323:Autoantibodies / Q000276:immunology; D001324:Autoantigens / Q000276:immunology; D002352:Carrier Proteins; D002417:Cattle; D002478:Cells, Cultured; D003094:Collagen; D003315:Cornea / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D003598:Cytoskeletal Proteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D051180:Desmoplakins; D003896:Desmosomes / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D000071821:Dystonin; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry / Q000379:methods; D008970:Molecular Weight; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D010455:Peptides / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D000097805:Collagen Type XVII
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D001324:Autoantigens; D002352:Carrier Proteins; D003094:Collagen; D003598:Cytoskeletal Proteins; D051180:Desmoplakins; D000071821:Dystonin; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D010455:Peptides; D000097805:Collagen Type XVII; C486268:DST protein, human