title: Spontaneous and induced activation of rat oocytes.
abstract: Ovulated rat oocytes undergo spontaneous activation during in vitro culture. After extrusion of the second polar body, they do not enter interphase but are arrested again in next metaphase-like stage (M III arrest). The present study demonstrates that puromycin and chloral hydrate can trigger transition to interphase of metaphase II and spontaneously (incompletely) activated rat oocytes. The response of oocytes to these activators depends on their stage at the time of application of a stimulus. Metaphase II oocytes enter interphase at 86.8% when treated with puromycin and in 28.7% after chloral hydrate activation. Oocytes activated with chloral hydrate at the time of spontaneously induced anaphase II enter interphase at 64.8%, but after reaching the stage of telophase II their capability to shift to interphase is again low (28.8%). Finally, M III oocytes cannot be forced to enter interphase by either chloral hydrate or puromycin treatment. This study shows that resumption of the second meiotic division and transition to interphase--the two processes that normally occur in succession as a response to oocyte activatin--can be experimentally separated.
authors.affiliation: Department of Embryology, Institute of Zoology, University of Warsaw, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Zernicka-Goetz
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002697:Chloral Hydrate / Q000494:pharmacology*; D005260:Female; D008540:Meiosis; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects; D010312:Parthenogenesis; D011691:Puromycin / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D002697:Chloral Hydrate; D011691:Puromycin