Chromosome anomalies in human oocytes in vitro.
OBJECTIVE
To determine possible effects on chromosomal status of human oocytes in vitro.


METHODS
A prospective cytogenetic study of human oocytes fixed at 4, 20 (noninseminated oocytes), or approximately 52 hours after pick-up (inseminated-unfertilized oocytes).


METHODS
Primary treatment of infertility in an institutional practice.


METHODS
Eighty-eight consecutive in vitro fertilization (IVF) patients (262 inseminated-unfertilized oocytes) and 47 IVF patients with greater than 8 oocytes retrieved (95 noninseminated oocytes).


METHODS
Ultrasound-guided transvaginal follicular aspiration.


METHODS
Planned before data collection began.


RESULTS
The incidence of chromosome anomalies did not differ between groups. However, a negative correlation between oocytes apparently without chromosomes and oocytes with fragmented chromosomes was found.


CONCLUSIONS
Incubation of oocyte in vitro does not affect the chromosome complement. The mechanisms leading to oocytes apparently without chromosomes and with fragmented chromosomes are self-excluding.
Tarín
J J
JJ
Instituto Valenciano de Infertilidad, Valencia University School of Medicine, Spain.
Gómez
E
E
Pellicer
A
A
D002869:Chromosome Aberrations; D005260:Female; D005307:Fertilization in Vitro; D006238:Haploidy; D006801:Humans; D007246:Infertility / Q000628:therapy; D009865:Oocytes / Q000648:ultrastructure*