Frog brain expresses a 60 KDa Ca2+ binding protein similar to mammalian calreticulin.
The present report was undertaken in an effort to characterize the nature of Ca2+ binding protein(s) in the central nervous system of less evolved vertebrates. In particular we investigated whether the brain microsomal fraction of Rana esculenta expresses calsequestrin, calreticulin and/or other related Ca2+ binding protein(s). We found that a 60 KDa protein having an NH2-terminal amino acid sequence similar to mammalian calreticulin is the major microsomal Ca2(+)-binding protein.
Trevesø
S
S
Istituto di Patologia Generale, Università di Ferrara, Italy.
Zorzato
F
F
Chiozzi
P
P
Melandri
P
P
Volpe
P
P
Pozzan
T
T
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000502:physiology; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D037282:Calreticulin; D002155:Calsequestrin / Q000737:chemistry / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D011893:Rana esculenta / Q000502:physiology*; D013045:Species Specificity
D002135:Calcium-Binding Proteins; D037282:Calreticulin; D002155:Calsequestrin