Distribution and characterization of dihydrodiol dehydrogenases in mammalian ocular tissues.
The immunological relationship of two forms of dihydrodiol dehydrogenase (DD) in pig lens to pig muscle aldose reductase and kidney aldehyde reductase has been studied. Although the minor enzyme form, a monomer of Mr 35,000, was identical with aldose reductase, the major enzyme form, a dimer of Mr 65,000, was distinct from the two reductases. The two enzyme species, although their amounts were low, were distributed in the cornea, iris-ciliary body, retina and choroid of the pig eye. In other mammals, rabbit lens exhibited much higher DD activity than did lens of mice, rats, cats, hamsters, guinea pigs and monkeys, and contained large amounts of the Mr-65,000 enzyme form as well as the minor enzyme form of Mr 35,000. In contrast, only the Mr-35,000 form of the enzyme was found in the lens of other species, except that a small amount of the high-Mr enzyme was detected in mouse lens. The high-Mr enzyme, purified from rabbit lens, was functionally and immunologically similar to dimeric DD of pig lens. The low-Mr enzyme forms, isolated or partially purified from these animal lenses, showed several features in common with aldose reductases from mammalian tissues. The dimeric enzymes of pig and rabbit lenses were NADP(+)-specific, whereas the low-Mr enzymes exhibited dual cofactor specificity and their activities with NAD+ were more than 3-fold higher than those with NADP+.
Hara
A
A
Department of Biochemistry, Gifu Pharmaceutical University, Japan.
Nakayama
T
T
Harada
T
T
Kanazu
T
T
Shinoda
M
M
Deyashiki
Y
Y
Sawada
H
H
D000429:Alcohol Oxidoreductases / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002415:Cats; D006224:Cricetinae; D005123:Eye / Q000201:enzymology*; D005260:Female; D006168:Guinea Pigs; D007908:Lens, Crystalline / Q000201:enzymology; D008251:Macaca; D046911:Macromolecular Substances; D008297:Male; D008647:Mesocricetus; D051379:Mice; D008970:Molecular Weight; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D013552:Swine; D014018:Tissue Distribution
D046911:Macromolecular Substances; D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; C023125:dihydrodiol dehydrogenases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C116788:cis-1,2-dihydro-1,2-dihydroxynaphthalene dehydrogenase