Propagation of human corneal endothelium in vitro effect of growth factors.
Endothelial cells were obtained from 23 human corneal-scleral rims. Donors were 0.2-37 yr of age. Most cultures obtained from donors under 30 yr ceased to grow after the seventh passage (3-24 weeks). Growth from 17- and 25-yr-old donors were maintained for nine passages (26 weeks) and 18 passages (100 weeks), respectively. Cultures were established from 12 rims, using growth factors in uncoated culture flasks or epidermal growth factor (EGF) in an extracellular matrix-coated (ECM) flask. EGF improved the growth potential of the cells; a combination of an ECM-coated-tissue culture flask with EGF in the medium provided an improved environment for continuous growth.
Samples
J R
JR
Ophthalmology Research Laboratory, Sharp Cabrillo Hospital, San Diego, CA 92110.
Binder
P S
PS
Nayak
S K
SK
D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D016228:Endothelial Growth Factors / Q000494:pharmacology; D004728:Endothelium, Corneal / Q000166:cytology / Q000187:drug effects*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D007223:Infant; D008938:Mitosis / Q000187:drug effects; D009414:Nerve Growth Factors / Q000494:pharmacology; D013997:Time Factors
D016228:Endothelial Growth Factors; D006133:Growth Substances; D009414:Nerve Growth Factors; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor