High- and low-voltage activated calcium currents are expressed by neurons cultured from embryonic rat neostriatum.
Current-clamp studies have shown that voltage-dependent Ca currents are present in rat neostriatal neurons. Although these studies have provided evidence for the presence of high-voltage activated Ca channels, it has been unclear whether low-voltage activated channels are also present. Using the whole-cell variant of the patch-clamp technique, we have studied isolated Ca currents in an attempt to answer this question. We have found that both high- and low-voltage activated calcium currents are expressed by neostriatal neurons cultured from embryonic rat brain. These currents are similar in voltage-dependence and pharmacology to those found in other brain neurons.
Bargas
J
J
Department of Anatomy and Neurobiology, College of Medicine, University of Tennessee, Memphis 38163.
Surmeier
D J
DJ
Kitai
S T
ST
D000818:Animals; D002118:Calcium / Q000502:physiology*; D002413:Cations, Divalent / Q000494:pharmacology; D003342:Corpus Striatum / Q000166:cytology / Q000502:physiology*; D004056:Differential Threshold; D004594:Electrophysiology; D009474:Neurons / Q000502:physiology*; D051381:Rats / Q000196:embryology; D013997:Time Factors
D002413:Cations, Divalent; D002118:Calcium