title: Release of periplasmic proteins induced in E. coli by expression of an N-terminal proximal segment of the phage fd gene 3 protein.
abstract: We used the enzymes beta-lactamase and alkaline phosphatase to quantitatively evaluate the release of periplasmic proteins from E. coli cells transformed by plasmids harboring gene 3 of phage fd. Different deletion mutants of gene 3 released varying fractions of the enzymes. From these results we conclude that essentially the amino-terminal proximal part, upstream of the first glycine-rich region but not this region itself, is responsible for the excretion of periplasmic proteins in E. coli cells expressing the gene 3 protein of phage fd.
authors.affiliation: Fakultät für Biologie, Universität Konstanz, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Rampf
authors.forename: P
authors.initials: P
authors.lastname: Bross
authors.forename: T
authors.initials: T
authors.lastname: Vocke
authors.forename: I
authors.initials: I
authors.lastname: Rasched
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D036022:Capsid Proteins; D003090:Coliphages / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D015870:Gene Expression; D005998:Glycine / Q000737:chemistry; D009154:Mutation; D010957:Plasmids; D014759:Viral Envelope Proteins / Q000235:genetics*; D014760:Viral Fusion Proteins; D014764:Viral Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D001618:beta-Lactamases / Q000378:metabolism
chemical_list: D036022:Capsid Proteins; C024013:DNA replication complex protein, Bacteriophage lambda; D004268:DNA-Binding Proteins; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D014764:Viral Proteins; D000469:Alkaline Phosphatase; D001618:beta-Lactamases; D005998:Glycine