Effects of ovarian steroids on energy balance in rats fed a highly palatable diet.
The effects of progesterone and estradiol on body weight, energy intake, energy expenditure, body composition, and brown adipose tissue activity were investigated in female rats fed a highly palatable diet (association of chow and full milk with sugar), which, by itself, induced an increase in food intake and energy expenditure. Progesterone and estradiol were administered in the form of implants. Ovariectomized animals were used in the estradiol studies. Energy expenditure was assessed through oxygen consumption, body composition through carcass analysis, brown adipose tissue activity through measurements of uncoupling-protein, guanosine diphosphate (GDP) binding capacity, and assay of uncoupling-protein mRNA. Body weight and food intake were increased by progesterone and decreased by estradiol. Energy expenditure was not altered by progesterone. Indirect evidence showed that estradiol increased energy expenditure, but direct measurements showed no modification. Changes in body weight under progesterone or under estradiol were not due to brown adipose tissue activity. The results indicate that ovarian hormones act on energy balance mainly by altering food intake, and possibly in the case of estradiol by increasing energy expenditure. These effects persist in rats fed a highly palatable diet, despite increases in energy intake and expenditure induced by the diet alone.
Guyard
B
B
INSERM U 286, Human Nutrition, Faculte de Medecine X, Bichat, Paris, France.
Fricker
J
J
Brigant
L
L
Betoulle
D
D
Apfelbaum
M
M
D002001:Adipose Tissue, Brown / Q000378:metabolism; D000821:Animal Feed; D000818:Animals; D004734:Energy Metabolism / Q000187:drug effects*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D010052:Ovariectomy; D011374:Progesterone / Q000494:pharmacology*; D051381:Rats
D011374:Progesterone; D004958:Estradiol