Isolation and characterization of hemorrhagic toxin from the venom of Trimeresurus elegans.
1. Hemorrhagic toxin from the venom of Trimeresurus elegans was purified in a homogeneous form using gel filtration and ion exchange chromatographies. 2. Hemorrhagic toxin possessed hemorrhagic and TAME (tosyl-L-arginine methyl ester) hydrolytic activities. These activities were inhibited when hemorrhagic toxin was incubated with benzamidine or N-bromosuccinimide. 3. Its mol. wt was 28,500 and the isoelectric point was 7.4. 4. This toxin contains ca 1.5 mol Ca per mol of protein.
Nikai
T
T
Department of Microbiology, Faculty of Pharmacy, Meijo University, Nagoya, Japan.
Komori
Y
Y
Imai
K
K
Sugihara
H
H
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003435:Crotalid Venoms / Q000032:analysis* / Q000302:isolation & purification* / Q000378:metabolism; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity
D000596:Amino Acids; D003435:Crotalid Venoms; C029748:Trimeresurus venoms; C068677:hemorrhagic toxin, Trimeresurus elegans; D010447:Peptide Hydrolases; C023073:tosylarginine methyl ester hydrolase