Proportional equilibration of K, Na ions, and sucrose molecules in pig lenses incubated in the presence of the non-ionic detergent Triton X-100.
The release of sodium and potassium and the uptake of sucrose molecules was studied in pig lenses incubated in isosmotic sucrose solution in either the presence or absence of 1% Triton X-100 (a non-ionic detergent). This Triton X-100 treatment has been shown to cause severe disruptions of cell membrane integrity. If sodium and potassium were free in the lens fibers as in a dilute aqueous solution, they would be expected to diffuse three to four times faster than sucrose. However, measurements of sodium and potassium release and sucrose uptake in the Triton X-100 treated lenses show a 1:1 equilibration. When pig lenses were incubated in the same solution without detergent, the sucrose uptake was significantly less than the potassium and sodium release. It is postulated that a slow, detergent mediated collapse of protein-water-ion interactions within the lens is the rate-limiting step of the observed equilibration of monovalent cations and sucrose molecules.
Miseta
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A
Department of Clinical Chemistry, Medical University of Pecs, Hungary.
Kellermayer
M
M
Ludany
A
A
Cameron
I L
IL
Hazlewood
C F
CF
D000818:Animals; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D046508:Culture Techniques; D007908:Lens, Crystalline / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011188:Potassium / Q000493:pharmacokinetics*; D012964:Sodium / Q000493:pharmacokinetics*; D013395:Sucrose / Q000493:pharmacokinetics*; D013552:Swine / Q000378:metabolism / Q000502:physiology; D014882:Water-Electrolyte Balance / Q000187:drug effects / Q000502:physiology
D011092:Polyethylene Glycols; D013395:Sucrose; D017830:Octoxynol; D012964:Sodium; D011188:Potassium