Bone-inducing activity of mature BMP-2b produced from a hybrid BMP-2a/2b precursor.
The human osteoinductive proteins BMP-2a and BMP-2b have been cloned and expressed in mammalian cells. In order to improve expression levels we examined the role of the proregion in assembly and export. Use of the BMP-2a proregion combined with the mature region of BMP-2b leads to dramatically improved expression of mature BMP-2b. Mature BMP-2b has been purified to near homogeneity from the BMP-2a/2b hybrid, and its structural properties and biological activity determined. Recombinant mature BMP-2b homodimer elicits bone formation in vivo.
Hammonds
R G
RG
Department of Developmental Biology, Genentech, Inc., South San Francisco, California 94080.
Schwall
R
R
Dudley
A
A
Berkemeier
L
L
Lai
C
C
Lee
J
J
Cunningham
N
N
Reddi
A H
AH
Wood
W I
WI
Mason
A J
AJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001846:Bone Development / Q000187:drug effects*; D019485:Bone Morphogenetic Proteins; D001842:Bone and Bones / Q000187:drug effects; D002460:Cell Line; D003001:Cloning, Molecular; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics*; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000494:pharmacology; D014162:Transfection
D019485:Bone Morphogenetic Proteins; D011498:Protein Precursors; D011506:Proteins; D011993:Recombinant Fusion Proteins