[Rapid identification methods of yeasts during fungaemia].
A wet preparation method, modified serum test and a modified disc diffusion method were evaluated in order to identify yeasts more rapidly during fungaemia. A total of 2,932 blood cultures were processed, of which 54 (1.8%) yielded yeasts. By using these methods, Candida albicans was identified within 3 hours, after yeast growth in the blood culture was confirmed by Gram stain. The wet preparation examinations were accurate in 60% of cases and the serum test in 95% of cases. After growth was detected, yeast species other than C. albicans were identified within 24 hours using the modified disc diffusion method. C. albicans (50%), C. tropicalis (22%) and C. parapsilosis (22%) were the species most frequently isolated. Fungaemia occurred most often after antimicrobial therapy and abdominal conditions.
Badenhorst
L
L
Departement Mediese Mikrobiologie, Universiteit van die Oranje-Vrystaat, Bloemfontein.
Botha
P L
PL
van Rensburg
M N
MN
D001769:Blood / Q000382:microbiology*; D002175:Candida / Q000302:isolation & purification; D002176:Candida albicans / Q000302:isolation & purification*; D006801:Humans; D008828:Microbiological Techniques; D009181:Mycoses / Q000175:diagnosis; D013997:Time Factors