title: Transformation of the filamentous fungus Gibberella fujikuroi using the Aspergillus niger niaD gene encoding nitrate reductase.
abstract: A transformation system for Gibberella fujikuroi based on the Aspergillus niger nitrate reductase gene (niaD) was developed. A strain (designated SG140) carrying a non-reverting niaD mutation (niaD11) was generated by screening mutagenised cells for non-growth on nitrate as sole nitrogen source. Transformation frequencies of 1-2 transformants per microgram DNA were observed when strain SG140 was transformed to nitrate utilisation. Southern blot analyses of niaD+ transformants showed that the vector DNA sequences were integrated into the chromosomal DNA. The results demonstrate that the A. niger niaD gene is expressed in G. fujikuroi.
authors.affiliation: Departamento de Genetica y Biotecnia, Universidad de Sevilla, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Sànchez-Fernàndez
authors.forename: S E
authors.initials: SE
authors.lastname: Unkles
authors.forename: E I
authors.initials: EI
authors.lastname: Campbell
authors.forename: J A
authors.initials: JA
authors.lastname: Macro
authors.forename: E
authors.initials: E
authors.lastname: Cerdà-Olmedo
authors.forename: J R
authors.initials: JR
authors.lastname: Kinghorn
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology / Q000235:genetics*; D015139:Blotting, Southern; D005874:Gibberella / Q000235:genetics*; D016296:Mutagenesis; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D014170:Transformation, Genetic
chemical_list: D009565:Nitrate Reductases; D050901:Nitrate Reductase