Relative rates of biosynthesis of collagen type I, type V and type VI in calf cornea.
The biosynthesis of type I, type V and type VI collagens was studied by incubation of calf corneas in vitro with [3H]proline as a marker. Pepsin-solubilized collagen types were isolated by salt fractionation and quantified by SDS/PAGE. Expressed as proportions of the total hydroxyproline solubilized, corneal stroma comprised 75% type I, 8% type V and 17% type VI collagen. The rates of [3H]proline incorporation, linear up to 24 h for each collagen type, were highest for type VI collagen and lowest for type I collagen. From pulse-chase experiments, the calculated apparent half-lives for types I, V and VI collagens were 36 h, 10 h and 6 h respectively.
Kern
P
P
Laboratoire de Biochimie du Tissu Conjonctif, UA CNRS 1174, Faculté de Médecine, Université Paris XII, France.
Menasche
M
M
Robert
L
L
D000818:Animals; D002417:Cattle; D003094:Collagen / Q000096:biosynthesis* / Q000302:isolation & purification; D003315:Cornea / Q000378:metabolism*; D007700:Kinetics; D011392:Proline / Q000378:metabolism; D011865:Radioisotope Dilution Technique; D014316:Tritium
D014316:Tritium; D003094:Collagen; D011392:Proline