Hypothalamic microdialysis of mazindol causes anorexia with increase in synaptic serotonin in rats.
Brain microdialysis was used to determine if lateral hypothalamic serotonin (5-HT) is involved in the mazindol-induced anorexia in rats. Direct application of mazindol through a microdialysis membrane both significantly increased the 5-HT levels in the lateral hypothalamus and suppressed food intake by fasted rats. The increase in 5-HT concentration was dose related and the concentration of mazindol for half-maximal response was 4.6 microM. Pretreatment with methysergide, a potent 5-HT receptor-blocking agent, significantly antagonized the mazindol-induced anorexia. These results suggest that the anorectic action of mazindol might be mediated, at least in part, through serotonergic mechanisms in the hypothalamus.
Shimizu
N
N
Department of Physiology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Take
S
S
Hori
T
T
Oomura
Y
Y
D000818:Animals; D001066:Appetite / Q000187:drug effects*; D002940:Circadian Rhythm / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004435:Eating / Q000187:drug effects*; D005247:Feeding Behavior / Q000187:drug effects; D007026:Hypothalamic Area, Lateral / Q000187:drug effects*; D008297:Male; D008454:Mazindol / Q000494:pharmacology*; D008784:Methysergide / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism*
D012701:Serotonin; D008454:Mazindol; D008784:Methysergide