A DNA probe for the detection of Mycobacterium paratuberculosis.
A genomic library of DNA extracted from Mycobacterium paratuberculosis was constructed in the expression vector lambda gt 11. The library was screened by plaque hybridization with labelled M. paratuberculosis genomic DNA as probe. Strongly hybridizing plaques were isolated and their DNA extracted and characterised for M. paratuberculosis specificity by hybridization to DNA from other Mycobacteriaceae. A clone was obtained which was specific for M. paratuberculosis. DNA from this clone could detect 7 ng M. paratuberculosis DNA.
Ambrosio
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RE
Molecular Biology Section, Veterinary Research Institute, Onderstepoort, South Africa.
Harris
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Y
Huchzermeyer
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HF
D000818:Animals; D003001:Cloning, Molecular; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D015723:Gene Library; D009161:Mycobacterium / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D010283:Paratuberculosis / Q000175:diagnosis / Q000382:microbiology*; D013045:Species Specificity
D015342:DNA Probes; D004269:DNA, Bacterial