title: High-volume enzyme immunoassay test system for sulfamethazine in swine.
abstract: A high-volume enzyme immunoassay (EIA) system for slaughterhouse screening of sulfamethazine in swine plasma/serum has been developed. The system includes a robotic sample processor that performs most of the liquid handling requirements in the assay. The assay gives good correlation with the widely used thin layer chromatographic method for determination of sulfamethazine in serum and plasma (r = 0.826). Inter- and intra-assay coefficients of variation are less than 10%. Approximately 2,400 serum/plasma samples can be analyzed in a normal working day (8 h) with this system.
authors.affiliation: Idetek, Inc, San Bruno, CA 94066.
authors.forename: B P
authors.initials: BP
authors.lastname: Ram
authors.forename: P
authors.initials: P
authors.lastname: Singh
authors.forename: L
authors.initials: L
authors.lastname: Martins
authors.forename: T
authors.initials: T
authors.lastname: Brock
authors.forename: N
authors.initials: N
authors.lastname: Sharkov
authors.forename: D
authors.initials: D
authors.lastname: Allison
mesh_terms: D000818:Animals; D002855:Chromatography, Thin Layer; D007124:Immunoenzyme Techniques; D008460:Meat / Q000032:analysis; D012015:Reference Standards; D013418:Sulfamethazine / Q000032:analysis* / Q000097:blood / Q000276:immunology; D013552:Swine; D013954:Thyroglobulin / Q000032:analysis / Q000276:immunology
chemical_list: D013418:Sulfamethazine; D013954:Thyroglobulin