title: Rapid aggregation and tight junction formation in single cell suspensions of tumor cells after very low dose trypsin treatment.
abstract: The possible participation of tight junction formation in intercellular adhesion of tumor cells was studied in single cell suspensions of HT29 adenocarcinoma cells. Very low dose trypsin treatment (0.15 micrograms/ml, 30 min, 37 degrees C) induced rapid intercellular adhesion of suspended HT29 colon carcinoma cells. Intercellular adhesion was independent of the presence of divalent cations. Electron microscopy of freeze-fractured membrane fragments of trypsin-treated HT29 cells revealed a dramatic increase of typical tight junction structures during aggregation.
authors.affiliation: Institut für Biochemie II, Universität Heidelberg, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Kemmner
authors.forename: K
authors.initials: K
authors.lastname: Zaar
mesh_terms: D000230:Adenocarcinoma; D002448:Cell Adhesion; D002449:Cell Aggregation; D002460:Cell Line; D003110:Colonic Neoplasms; D005614:Freeze Fracturing; D006801:Humans; D007365:Intercellular Junctions / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D014357:Trypsin / Q000494:pharmacology*
chemical_list: D014357:Trypsin