title: Cytogenetic and flow cytometric DNA analysis in renal cell carcinoma.
abstract: Cytogenetic and flow cytometric DNA analyses were performed on 37 tumor samples from 16 patients with renal cell carcinoma. 36 cultures were chromosomally abnormal and 1 was normal. The most frequent abnormality was trisomy or tetrasomy of chromosome 7 (13 of 16 tumors), suggesting that this abnormality might be a primary change in renal cell carcinoma. Abnormalities of chromosome 3 were seen in 9 of 16 tumors (56%). Eight of 11 tumors (73%) with cytogenetic results from multiple tumor samples showed intratumor cytogenetic heterogeneity. Twelve of the 16 patients had aneuploid tumors, and 4 had homogeneously diploid tumors. DNA index correlated with chromosome number. No association was found between cytogenetic results and clinical stage.
authors.affiliation: Department of Urology, University of Umeå, Sweden.
authors.forename: B
authors.initials: B
authors.lastname: Ljungberg
authors.forename: I
authors.initials: I
authors.lastname: Nordenson
authors.forename: G
authors.initials: G
authors.lastname: Roos
mesh_terms: D000782:Aneuploidy; D002292:Carcinoma, Renal Cell / Q000235:genetics*; D002893:Chromosomes, Human, Pair 3; D002897:Chromosomes, Human, Pair 7; D004273:DNA, Neoplasm / Q000032:analysis*; D004171:Diploidy; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007621:Karyotyping; D007668:Kidney / Q000648:ultrastructure*; D007680:Kidney Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D014314:Trisomy; D014407:Tumor Cells, Cultured
chemical_list: D004273:DNA, Neoplasm