title: Transformation of human breast epithelial cells by c-Ha-ras oncogene.
abstract: To determine whether the c-Ha-ras oncogene plays a role in the initiation of mammary carcinogenesis, an immortalized human breast epithelial cell line, MCF-10A, was transfected with the plasmid vector pHo6T1 containing the T24 Ha-ras oncogene and the aminoglycoside phosphotransferase gene, which confers resistance to geneticin. Transfected cells exhibited an altered pattern of growth and tridimensional morphology in collagen gel. They also exhibited anchorage-independent growth and loss of requirement for hormones and epidermal growth factor; in addition, they expressed invasiveness and increased collagenolytic activity in an in vitro system and became tumorigenic in irradiated nude mice, all properties indicative of malignant transformation. Transformed cells contained the mutated c-Ha-ras oncogene and expressed the p21 mutated protein. These data indicate that the c-Ha-ras oncogene is capable of inducing malignant phenotypes in immortalized human breast epithelial cells.
authors.affiliation: Michigan Cancer Foundation, Detroit 48201.
authors.forename: F
authors.initials: F
authors.lastname: Basolo
authors.forename: J
authors.initials: J
authors.lastname: Elliott
authors.forename: L
authors.initials: L
authors.lastname: Tait
authors.forename: X Q
authors.initials: XQ
authors.lastname: Chen
authors.forename: T
authors.initials: T
authors.lastname: Maloney
authors.forename: I H
authors.initials: IH
authors.lastname: Russo
authors.forename: R
authors.initials: R
authors.lastname: Pauley
authors.forename: S
authors.initials: S
authors.lastname: Momiki
authors.forename: J
authors.initials: J
authors.lastname: Caamano
authors.forename: A J
authors.initials: AJ
authors.lastname: Klein-Szanto
mesh_terms: D001940:Breast / Q000473:pathology*; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D011905:Genes, ras; D006801:Humans; D007621:Karyotyping; D014162:Transfection; D014410:Tumor Stem Cell Assay