Random segregation of chromatids at mitosis in Saccharomyces cerevisiae.
Previous experiments suggest that mitotic chromosome segregation in some fungi is a nonrandom process in which chromatids of the same replicative age are destined for cosegregation. We have investigated the pattern of chromatid segregation in Saccharomyces cerevisiae by labeling the DNA of a strain auxotrophic for thymidine with 5-bromodeoxyuridine. The fate of DNA strands was followed qualitatively by immunofluorescence microscopy and quantitatively by microphotometry using an anti-5-bromodeoxyuridine monoclonal antibody. Chromatids of the same replicative age were distributed randomly to daughter cells at mitosis. Quantitative measurements showed that the amount of fluorescence in the daughter nuclei derived from parents with hemilabeled chromosomes diminished in intensity by one half. The concentration of 5-bromodeoxyuridine used in the experiments had little effect on the frequency of either homologous or sister chromatid exchanges. We infer that the 5-bromodeoxyuridine was distributed randomly due to mitotic segregation of chromatids and not via sister chromatid exchanges.
Neff
M W
MW
Department of Biology, University of Virginia, Charlottesville 22901.
Burke
D J
DJ
D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine / Q000378:metabolism; D002455:Cell Division; D002842:Chromatids / Q000502:physiology*; D004261:DNA Replication; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D008856:Microscopy, Fluorescence; D008938:Mitosis; D010783:Photometry; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000254:growth & development; D012854:Sister Chromatid Exchange
D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine