Superiority of the use of a pre-incubated complex of primary and second antibody in the radioimmunoassay of thyroid hormones.
We report on the superiority of a radioimmunoassay (RIA) system wherein the second antibody (Ab2) is incorporated as a pre-incubated complex with the primary antibody (Ab1) for the assay of haptens like triiodothyronine (T3) and thyroxine (T4). Separation of the antibody bound and free antigen (Ag) was accomplished by 8% polyethylene glycol (PEG) (final concentration) following a single incubation of less than 1 h. The other advantages of this system are a 15-fold reduction in the quantity of Ab2 (without any need for increasing the concentration of PEG) and the consequent savings in cost.
Rathinaswamy
A
A
Board of Radiation and Isotope Technology, BARC Vashi Complex, New Bombay, India.
Nagvekar
U H
UH
Chatur
J
J
Nirmala
V
V
Rani
K
K
Ramamoorthy
N
N
Balakrishnan
S A
SA
Desai
C N
CN
D003365:Costs and Cost Analysis; D006801:Humans; D011863:Radioimmunoassay / Q000379:methods*; D011933:Reagent Kits, Diagnostic / Q000191:economics; D013963:Thyroid Hormones / Q000032:analysis*
D011933:Reagent Kits, Diagnostic; D013963:Thyroid Hormones