Characterization of a human TAR RNA-binding protein that activates the HIV-1 LTR.
Human immunodeficiency virus type 1 (HIV-1) gene expression is activated by Tat, a virally encoded protein. Tat trans-activation requires viral (trans-activation--responsive; TAR) RNA sequences located in the R region of the long terminal repeat (LTR). Existing evidence suggests that Tat probably cooperates with cellular factors that bind to TAR RNA in the overall trans-activation process. A HeLa complementary DNA was isolated and characterized that encodes a TAR RNA-binding protein (TRBP). TRBP activated the HIV-1 LTR and was synergistic with Tat function.
Gatignol
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A
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
Buckler-White
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Berkhout
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B
Jeang
K T
KT
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000235:genetics*; D004722:Endoribonucleases / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology; D029968:Escherichia coli Proteins; D015696:Gene Products, tat / Q000378:metabolism; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010957:Plasmids; D012367:RNA, Viral / Q000235:genetics; D016601:RNA-Binding Proteins; D043244:Ribonuclease III; D012689:Sequence Homology, Nucleic Acid; D054322:tat Gene Products, Human Immunodeficiency Virus; D000099253:Trans-Activation Responsive RNA-Binding Protein
D002352:Carrier Proteins; D004722:Endoribonucleases; D029968:Escherichia coli Proteins; D015696:Gene Products, tat; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D043244:Ribonuclease III; D054322:tat Gene Products, Human Immunodeficiency Virus; D000099253:Trans-Activation Responsive RNA-Binding Protein; C468366:ribonuclease III, E coli