title: A specific glutathione S-transferase isozyme occurring in hereditary hyperbilirubinuria rats.
abstract: A glutathione S-transferase isozyme which is absent in normal rat liver has been isolated from the hereditary hyperbilirubinuria rat liver cytosol. The enzyme was purified to apparent homogeneity by GSH-affinity chromatography and HPLC on CM-Sepharose CL-6B. It is a heterodimer of two non-identical subunits, i.e., subunit 2 and a previously uncharacterized subunit referred to here as subunit Yx. Immunoblot analysis indicated that GST 2-Yx belongs to the alpha class. GST 2-Yx is characterized by its 4-fold higher activity towards 4-hydroxy-non-2-enal, compared to that of GST 2-2.
authors.affiliation: Laboratory of Biochemical Pharmacology and Biotoxicology, Faculty of Pharmaceutical Sciences, Chiba University.
authors.forename: T
authors.initials: T
authors.lastname: Igarashi
authors.forename: T
authors.initials: T
authors.lastname: Tsuchiya
authors.forename: T
authors.initials: T
authors.lastname: Satoh
mesh_terms: D000818:Animals; D001663:Bilirubin / Q000652:urine*; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005982:Glutathione Transferase / Q000302:isolation & purification*; D006933:Hyperbilirubinemia, Hereditary / Q000201:enzymology* / Q000652:urine; D015151:Immunoblotting; D007527:Isoenzymes / Q000302:isolation & purification*; D008099:Liver / Q000201:enzymology; D051381:Rats
chemical_list: D007527:Isoenzymes; D005982:Glutathione Transferase; D001663:Bilirubin