title: Interstrand cross-linking of the hexadeoxynucleotide d(TACGTA) upon reaction with trans-diamminedichloroplatinum(II).
abstract: The reaction of trans-diamminedichloroplatinum(II), an inactive isomer of cisplatin, with d(TACGTA) was performed in aqueous solution, and the interstrand cross-linked adduct was isolated on a preparative scale. This adduct was characterized by 1 M thiourea treatment, atomic absorption spectroscopy, enzymatic digestion and 1H NMR as platinum coordinated to two molecules of d(TACGTA) at the N7 atom of the guanine residue. This interstrand cross-linked adduct did not form a duplex structure in the conditions where the free hexanucleotide adopts it.
authors.affiliation: Osaka University of Pharmaceutical Sciences, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Urata
authors.forename: M
authors.initials: M
authors.lastname: Ueno
authors.forename: F
authors.initials: F
authors.lastname: Yamasaki
authors.forename: M
authors.initials: M
authors.lastname: Akagi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002945:Cisplatin / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004249:DNA Damage; D006147:Guanine / Q000737:chemistry; D006860:Hydrogen Bonding; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D003432:Cross-Linking Reagents; D009838:Oligodeoxyribonucleotides; C034697:transplatin; D006147:Guanine; D002945:Cisplatin