Modulation of basement membrane component gene expression in glomeruli of aminonucleoside nephrosis.
The present studies were designed to analyze mRNA levels of basement membrane components including collagen IV, laminin, and heparan sulfate proteoglycan (HSPG) in the course of puromycin aminonucleoside (PAN) nephrosis. mRNA levels for alpha 1 (IV) chain; laminin A, B1, and B2 chains; and HSPG were measured in glomeruli of PAN nephrotic rats 0, 2, 8, 14, and 20 days after PAN injection. In the nephrotic stage of PAN nephrosis (on the 8th day), mRNA levels for alpha 1 (IV) chain and laminin A, B1, and B2 chains increased, whereas those for HSPG decreased. The anionic sites in glomerular basement membrane stained by polyethyleneimine were smaller in size and fewer in number in PAN nephrotic rats than they were in control rats. In the remission stage of PAN nephrosis (on the 20th day), however, mRNA levels for alpha 1 (IV) chain and laminin A, B1, and B2 chains decreased, whereas mRNA levels for HSPG increased. Polyethyleneimine aggregates in this stage appeared to be larger and more intense than those in the nephrotic stage. These results indicate that the expression of basement membrane genes for alpha 1 (IV), laminin, and HSPG was abnormally regulated in PAN nephrosis and that this abnormal gene regulation might contribute to the development of proteinuria.
Nakamura
T
T
Department of Medicine, Juntendo University School of Medicine, Tokyo, Japan.
Ebihara
I
I
Shirato
I
I
Tomino
Y
Y
Koide
H
H
D000818:Animals; D001485:Basement Membrane / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D015152:Blotting, Northern; D003094:Collagen / Q000235:genetics / Q000378:metabolism*; D015342:DNA Probes; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation / Q000502:physiology*; D006497:Heparan Sulfate / Q000235:genetics / Q000378:metabolism*; D007678:Kidney Glomerulus / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure; D007797:Laminin / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008854:Microscopy, Electron; D009401:Nephrosis / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D011094:Polyethyleneimine; D011507:Proteinuria / Q000209:etiology / Q000378:metabolism; D011692:Puromycin Aminonucleoside; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D003094:Collagen; D015342:DNA Probes; D006497:Heparan Sulfate; D007797:Laminin; D011094:Polyethyleneimine; D011692:Puromycin Aminonucleoside; D012333:RNA, Messenger