title: Interleukin-6 and interleukin-1 enhancement of GM-CSF-dependent proliferation of haematopoietic progenitor cells in myelodysplastic syndromes.
abstract: Interleukin-1 (Il-1) and Interleukin-6 (Il-6) have been reported to enhance the growth factor dependent colony formation of normal primitive haematopoietic progenitor cells as well as of leukaemic blast-cell progenitors. We investigated the effects of Il-1 beta and Il-6 in combination with granulocyte-macrophage colony-stimulating factor (GM-CSF) on the in vitro colony formation of myeloid progenitors from 23 patients with a myelodysplastic syndrome (MDS). Neither Il-1 beta nor Il-6 were found to have colony stimulating activity on their own. In normal bone marrow cultures, either stimulated with optimal or suboptimal doses of GM-CSF, no enhancing or antagonistic effect of Il-6 or Il-1 beta was detected. In a majority of the MDS cases, however, an enhancing effect of Il-6 and Il-1 beta in combination with GM-CSF was observed (20/23 and 10/21 cases respectively). In three cases of the Il-6 and GM-CSF combination an antagonistic effect was observed as well as in four cases of the Il-1 beta and GM-CSF combination. A delayed addition of Il-6 to the cultures did not result in an abrogation of the effect, indicating that Il-6 is not required immediately at the initiation of the culture. These results indicate that costimulation with Il-6 or Il-1 beta is able to augment the GM-CSF activity on MDS myeloid progenitor cells.
authors.affiliation: Department of Haematology, University Hospital, The Netherlands.
authors.forename: M R
authors.initials: MR
authors.lastname: Schipperus
authors.forename: P
authors.initials: P
authors.lastname: Sonneveld
authors.forename: J
authors.initials: J
authors.lastname: Lindemans
authors.forename: K
authors.initials: K
authors.lastname: van Lom
authors.forename: M
authors.initials: M
authors.lastname: Vlastuin
authors.forename: J
authors.initials: J
authors.lastname: Abels
mesh_terms: D000938:Antigen-Presenting Cells / Q000276:immunology; D001853:Bone Marrow / Q000473:pathology; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D007375:Interleukin-1 / Q000502:physiology*; D015850:Interleukin-6 / Q000502:physiology*; D009190:Myelodysplastic Syndromes / Q000276:immunology / Q000473:pathology*; D011994:Recombinant Proteins / Q000502:physiology
chemical_list: D007375:Interleukin-1; D015850:Interleukin-6; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor