Fractionation of egg and soybean phosphatidylcholines by silver resin chromatography.
Samples of egg and soybean phosphatidylcholines were fractionated by total number of double bonds on silver ion-saturated resin columns using solvent programming (acetonitrile in methanol). Stainless-steel columns were packed with 15-microns resin which had been isolated by air elutriation. Methods of column packing, silver ion incorporation, solvent-resin interactions and resin regeneration are also discussed.
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U.S. Department of Agriculture, Northern Regional Research Center, Peoria, IL 61604.
D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D004531:Eggs / Q000032:analysis*; D010713:Phosphatidylcholines / Q000302:isolation & purification*; D012834:Silver; D013025:Glycine max / Q000032:analysis*
D010713:Phosphatidylcholines; D012834:Silver