Calcium handling by platelets from normal and malignant hyperthermia-susceptible pigs.
Platelets from normal and malignant hyperthermia (MH)-susceptible pigs were evaluated for differences in 45calcium uptake in the absence or presence of caffeine (2-16 mM), halothane (0.05-0.5%), or halothane and caffeine together. There were no statistically significant differences in basal or halothane-inhibited calcium uptake by platelets from either source. There was a small statistically significant difference in calcium uptake between platelets from normal and MH-susceptible pigs in the presence of 16 mM caffeine and 0.5% halothane. Calcium uptake by platelets from one pedigree of MH-susceptible pigs were stimulated in a concentration-dependent manner by caffeine. These data suggest that exposure of platelets to caffeine may have potential for identifying MH-susceptibility.
Miller
K E
KE
Product Development, Norwich Eaton Pharmaceuticals, Inc., Norwich, NY 13815.
Brooks
R R
RR
Bonk
K R
KR
Carpenter
J F
JF
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001792:Blood Platelets / Q000378:metabolism*; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000097:blood*; D006221:Halothane / Q000494:pharmacology; D008305:Malignant Hyperthermia / Q000097:blood*; D013552:Swine
D002110:Caffeine; D002118:Calcium; D006221:Halothane