title: Effects of metal ion chelators on DNA strand breaks and inactivation produced by hydrogen peroxide in Escherichia coli: detection of iron-independent lesions.
abstract: In order to study the role of metallic ions in the H2O2 inactivation of Escherichia coli cells, H2O2-sensitive mutants were treated with metal ion chelators and then submitted to H2O2 treatment. o-Phenanthroline, dipyridyl, desferrioxamine, and neocuproine were used as metal chelators. Cell sensitivity to H2O2 treatment was not modified by neocuproine, suggesting that copper has a minor role in OH production in E. coli. On the other hand, prior treatment with iron chelators protected the cells against the H2O2 lethal effect, indicating that iron participates in the production of OH. However, analysis of DNA sedimentation profiles and DNA degradation studies indicated that these chelators did not completely block the formation of DNA single-strand breaks by H2O2 treatment. Thiourea, a scavenger of OH, caused a reduction in both H2O2 sensitivity and DNA single-strand break production. The breaks observed after treatment with metal chelators and H2O2 were repaired 60 min after H2O2 elimination in xthA but not polA mutant cells. Therefore, we propose that there are at least two pathways for H2O2-induced DNA lesions: one produced by H2O2 through iron oxidation and OH production, in which lesions are repaired by the products of the xthA and polA genes, and the other produced by an iron-independent pathway in which DNA repair requires polA gene products but not those of the xthA gene.
authors.affiliation: Departamento de Radiobiologia, Instituto de Biofísica Carlos, Chagas Filho, Universidade Federal de Rio de Janeiro, Brazil.
authors.forename: N R
authors.initials: NR
authors.lastname: Asad
authors.forename: A C
authors.initials: AC
authors.lastname: Leitão
mesh_terms: D015082:2,2'-Dipyridyl / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D004260:DNA Repair; D003676:Deferoxamine / Q000494:pharmacology; D004336:Drug Antagonism; D004926:Escherichia coli / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007502:Iron Chelating Agents / Q000494:pharmacology*; D010618:Phenanthrolines / Q000494:pharmacology
chemical_list: D007502:Iron Chelating Agents; D010618:Phenanthrolines; D015082:2,2'-Dipyridyl; C002701:neocuproine; D004247:DNA; D006861:Hydrogen Peroxide; D003676:Deferoxamine; C025205:1,10-phenanthroline