Proteolysis at the 2A/2B junction in Theiler's murine encephalomyelitis virus.
Processing at the 2A/2B junction in Theiler's murine encephalomyelitis virus (TMEV) was studied using in vitro transcription and translation. A plasmid which contained only the P2 region of TMEV was used. The protein products obtained after in vitro transcription/translation indicated that proteolysis at the 2A/2B junction occurs even if templates coding for only the 2A and 2B proteins are used. Time-course experiments indicated that 2A/2B proteolysis was cotranslational. The plasmid described will allow further definition of the sequences involved in this proteolytic event.
Batson
S
S
Northwestern University Medical School, Department of Microbiology-Immunology, Chicago, Illinois.
Rundell
K
K
D002678:Chimera; D006868:Hydrolysis; D007700:Kinetics; D004680:Maus Elberfeld virus / Q000201:enzymology / Q000235:genetics*; D010957:Plasmids; D011499:Protein Processing, Post-Translational; D012156:Reticulocytes / Q000378:metabolism; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics*
D014764:Viral Proteins; C057421:virus protein 2A