Cloning and characterization of gentamicin-resistance genes from Micromonospora purpurea and Micromonospora rosea.
Aminoglycoside-resistance genes (grm) were cloned from a gentamicin producer Micromonospora purpurea and a sisomicin producer Micromonospora rosea. The nucleotide (nt) sequences of both genes were determined and the similarity between them was very high (90.4% identity). In either case, the transcription start point was localised to about 11 nt upstream from the likely translation start codons of grm, which is expressed as a polycistronic transcript. In studies to be reported elsewhere, it has been established that the M. purpurea grm gene encodes a ribosomal RNA methyltransferase. Here, we confirmed that the similarity of the two genes exists not only at the structural but also at the functional level.
Kelemen
G H
GH
Department of Biochemistry, University of Leicester, U.K.
Cundliffe
E
E
Financsek
I
I
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial; D005839:Gentamicins / Q000494:pharmacology*; D008847:Micromonospora / Q000235:genetics*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D005839:Gentamicins