title: Fibroblasts inhibit mineralised bone nodule formation by rat bone marrow stromal cells in vitro.
abstract: The ability of rat skin fibroblasts (RSF) and human periodontal ligament fibroblasts (HPL) to inhibit the formation of mineralised bone nodules in rat bone marrow stromal cell (BMSC) cultures was studied. Co-culture of HPL or RSF with BMSC resulted in a large reduction of bone nodule formation when compared with controls. Conditioned medium from HPL or RSF cultures inhibited bone nodule formation in a dose-dependent manner. HPL-conditioned medium depressed cell proliferation and alkaline phosphatase expression in BMSC cultures. These effects were not due to increased cytotoxicity or nutrient depletion. Inhibitory activity was recovered in a fraction of less than 1 kD following ultrafiltration and was insensitive to freeze-thawing. The inhibitory activity was blocked when HPL cultures were grown in the presence of 10(-5) M indomethacin. Dose-dependent inhibiton of bone nodule formation was also observed in cultures incubated with prostaglandins E2 (at 10(-6) M) or F2 alpha (at 10(-7) M). The results indicate that fibroblasts may inhibit osteoblast differentiation and function in part by release of soluble factors including prostaglandins.
authors.affiliation: Faculty of Dentistry, University of Toronto, Ontario, Canada.
authors.forename: B
authors.initials: B
authors.lastname: Ogiso
authors.forename: F J
authors.initials: FJ
authors.lastname: Hughes
authors.forename: A H
authors.initials: AH
authors.lastname: Melcher
authors.forename: C A
authors.initials: CA
authors.lastname: McCulloch
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001853:Bone Marrow / Q000201:enzymology / Q000502:physiology; D001854:Bone Marrow Cells; D002113:Calcification, Physiologic / Q000187:drug effects* / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D015232:Dinoprostone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology; D010513:Periodontal Ligament / Q000166:cytology; D011453:Prostaglandins / Q000378:metabolism / Q000502:physiology; D051381:Rats; D012867:Skin / Q000166:cytology
chemical_list: D011453:Prostaglandins; D000469:Alkaline Phosphatase; D015232:Dinoprostone; D007213:Indomethacin