[Spectrofluorometric ultramicroassay for determining the plasma lipid peroxidation index by the thiobarbituric acid test].
A simple, inexpensive spectrofluorometric ultramicroassay for determining the plasma lipid peroxidation index is described. Plasma is incubated for 60 minutes at 95 degrees C in a thiobarbituric acetate buffer medium (pH 3,5) with butylhydroxytoluene and Fe . The chromogen is then extracted in butanol 1-pyridine and quantified by spectrofluorometry (exc.: 532 nm; em: 553 nm). The mean value of the plasma thiobarbituric lipid peroxidation index determined by this method in 101 healthy non-smokers (age 39.1 +/- 8.7 years) was found to be 1.61 +/- 0.72.
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Laboratoire de Biochimie Clinique, UFR de Médecine de l'Université Paris-Nord, Bobigny, France.
D000328:Adult; D002084:Butylated Hydroxytoluene; D006801:Humans; D007501:Iron; D008054:Lipid Peroxides / Q000097:blood*; D008875:Middle Aged; D012016:Reference Values; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013858:Thiobarbiturates
D008054:Lipid Peroxides; D013858:Thiobarbiturates; D002084:Butylated Hydroxytoluene; D007501:Iron