title: L-ascorbic acid 2-phosphate, a phosphate derivative of L-ascorbic acid, enhances the growth of cultured rabbit keratocytes.
abstract: We examined the effect of L-ascorbic acid 2-phosphate (P-Asc) on the proliferation of cultured rabbit keratocytes. P-Asc is a phosphate derivative of L-ascorbic acid and has more prolonged vitamin C activity in solution than does L-ascorbic acid. The proliferation of cultured keratocytes was promoted by the presence of P-Asc in culture medium. Transmission electron microscopic observations revealed that cells were more multi-layered after culture in the presence of P-Asc (0.1 mM) for 30 days than were those cultured in the absence of P-Asc. The effect of P-Asc was abrogated by L-azetidine 2-carboxylic acid, which is an analogue of proline that inhibits the production and secretion of collagen. Our observations support a therapeutic role for P-Asc in the repair of corneal stromal damage such as that caused by a corneal chemical burn.
authors.affiliation: Department of Ophthalmology, Wakayama Medical College, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Saika
authors.forename: R
authors.initials: R
authors.lastname: Kanagawa
authors.forename: K
authors.initials: K
authors.lastname: Uenoyama
authors.forename: K
authors.initials: K
authors.lastname: Hiroi
authors.forename: J
authors.initials: J
authors.lastname: Hiraoka
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000494:pharmacology; D001383:Azetidinecarboxylic Acid / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003319:Corneal Stroma / Q000187:drug effects* / Q000648:ultrastructure; D005260:Female; D015603:Keratinocytes / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D011817:Rabbits
chemical_list: C011669:ascorbate-2-phosphate; D001383:Azetidinecarboxylic Acid; D001205:Ascorbic Acid