In vitro fertilization and development of bovine oocytes matured in serum-free medium.
This study was undertaken to investigate the effects of supplementation of serum (fetal calf serum), gonadotropins (LH, FSH, prolactin) and estradiol-17 beta (E2) to culture medium during in vitro maturation of bovine cumulus oocyte complexes on subsequent fertilization and development to the blastocyst stage in vitro. Serum supplementation during bovine oocyte maturation was not required but hormonal supplementation, gonadotropins (LH + FSH) and E2, enhanced the fertilizability and developmental ability of bovine oocytes matured in vitro. The addition of prolactin to maturation medium containing LH, FSH, and E2 did not further enhance frequencies of fertilization and development.
Saeki
K
K
Department of Meat and Animal Science, University of Wisconsin, Madison 53706.
Hoshi
M
M
Leibfried-Rutledge
M L
ML
First
N L
NL
D000818:Animals; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D003470:Culture Media; D005314:Embryonic and Fetal Development / Q000187:drug effects; D005307:Fertilization in Vitro; D006728:Hormones / Q000494:pharmacology; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects
D003470:Culture Media; D006728:Hormones