title: Lymphoproliferative disease virus of turkeys: sequence analysis and transcriptional activity of the long terminal repeat.
abstract: The lymphoproliferative disease virus (LPDV) is the etiological agent of a lymphoproliferative disease that naturally occurs in turkeys. Recently, we have cloned the LPDV provirus and established it as a replication-competent genome devoid of a viral oncogene [Gak et al., J. Virol. 63 (1989) 2877-2880]. This report presents the nucleotide sequence of its long terminal repeat (LTR) and establishes it as a potent transcriptional element. Several features of the LPDV LTR were similar to those found in the LTRs of the avian sarcoma-leukemia viruses (ASLV) and include the primer-binding site (tRNATrp), the polypurine tract, the organization of the polyadenylation signal, the complexities of the U3, R and U5 regions, as well as a potential secondary structure in U5-R. The LTR sequence diverges significantly from the ASLV LTRs, which share a common structure and have extensive sequence homology mainly in the R and U5 domains. These findings support the conclusion that LPDV represents a distinct class of avian retrovirus, evolutionarily related to the ASLV family.
authors.affiliation: Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Israel.
authors.forename: E
authors.initials: E
authors.lastname: Gak
authors.forename: A
authors.initials: A
authors.lastname: Yaniv
authors.forename: L
authors.initials: L
authors.lastname: Sherman
authors.forename: M
authors.initials: M
authors.lastname: Ianconescu
authors.forename: S R
authors.initials: SR
authors.lastname: Tronick
authors.forename: A
authors.initials: A
authors.lastname: Gazit
mesh_terms: D000818:Animals; D001483:Base Sequence; D002701:Chloramphenicol / Q000096:biosynthesis; D008232:Lymphoproliferative Disorders / Q000382:microbiology* / Q000662:veterinary; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000382:microbiology*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014422:Turkeys
chemical_list: D002701:Chloramphenicol