title: Autoantibodies to nucleolin in systemic lupus erythematosus and other diseases.
abstract: The 110-kDa intracellular phosphoprotein (110K) described previously by this laboratory as a common IgM autoantigen in SLE and certain other systemic autoimmune disorders and viral infections is identified as nucleolin in the present investigation. Using rabbit antiserum to rat nucleolin as a probe, IgM autoantibody-reactive 110K co-migrated with human lymphocyte nucleolin in one- and two-dimensional immunoblots. Rabbit anti-nucleolin also specifically depleted autoreactive 110K from detergent lysates of human cells. Because nucleolin shares amino acid sequence similarity and/or forms dynamic particles with other prominent autoantigens, the present observation raises the possibility that the nucleolin/anti-nucleolin system may be of special significance for the development of humoral autoreactivity to nuclear Ag.
authors.affiliation: Third Department of Internal Medicine, University of Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Minota
authors.forename: W N
authors.initials: WN
authors.lastname: Jarjour
authors.forename: N
authors.initials: N
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Nojima
authors.forename: R A
authors.initials: RA
authors.lastname: Roubey
authors.forename: T
authors.initials: T
authors.lastname: Mimura
authors.forename: A
authors.initials: A
authors.lastname: Yamada
authors.forename: T
authors.initials: T
authors.lastname: Hosoya
authors.forename: F
authors.initials: F
authors.lastname: Takaku
authors.forename: J B
authors.initials: JB
authors.lastname: Winfield
mesh_terms: D000918:Antibody Specificity; D001323:Autoantibodies / Q000276:immunology*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D007526:Isoelectric Point; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000737:chemistry / Q000276:immunology*; D010750:Phosphoproteins / Q000737:chemistry / Q000276:immunology*; D011233:Precipitin Tests; D016601:RNA-Binding Proteins; D000097796:Nucleolin
chemical_list: D001323:Autoantibodies; D007075:Immunoglobulin M; D009687:Nuclear Proteins; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D000097796:Nucleolin