The association of haemagglutination and adhesion with lipopolysaccharide of Shigella dysenteriae serotype 1.
In this study the ability of strains of Shigella dysenteriae serotype 1 to agglutinate mammalian erythrocytes is attributed to the polysaccharide fraction of bacterial-cell lipopolysaccharide (LPS). LPS obtained from a rough, mutant strain of S. dysenteriae serotype 1, lacking the O-antigen polysaccharide side-chain, did not agglutinate erythrocytes, clearly demonstrating a link between O-antigen polysaccharides and haemagglutinating activity (HA). Strains of S. dysenteriae serotype 1 adhered well to cultured Henle Intestinal 407 cells, whereas rough strains adhered poorly. Pre-treatment of bacteria with LPS-specific antisera inhibited both HA and binding to cultured human-intestinal cells. The contribution of the polysaccharide side-chain and its associated HA--which appear to facilitate binding to cultured cells--to bacterial attachment to colonocytes and to the pathogenesis of shigellosis in vivo needs to be confirmed in animal studies.
Qadri
F
F
International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh.
Haq
S
S
Hossain
S A
SA
Ciznar
I
I
Tzipori
S
S
D001422:Bacterial Adhesion; D002460:Cell Line; D004848:Epithelium / Q000378:metabolism; D004912:Erythrocytes; D006384:Hemagglutination; D006388:Hemagglutinins / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D008070:Lipopolysaccharides / Q000378:metabolism*; D009077:Mucins / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D012762:Shigella dysenteriae / Q000378:metabolism*
D006388:Hemagglutinins; D008070:Lipopolysaccharides; D009077:Mucins; D011134:Polysaccharides