title: Renal and systemic plasma immunoreactive neuropeptide Y and calcitonin gene-related peptide responses to mental stress and adrenaline in humans.
abstract: Arterial plasma levels and renal venous overflows of immunoreactive neuropeptide Y (NPY-LI) and calcitonin gene-related peptide (CGRP-LI) were studied during mental stress (a modified version of Stroop's colour word conflict test--CWT) and during i.v. infusions of step-wisely increasing doses of adrenaline (ADR) in 12 healthy male volunteers. At rest, mean arterial plasma levels of NPY-LI and CGRP-LI were 27 +/- 2 pmol l-1 and 61 +/- 9 pmol l-1, respectively. No renal venoarterial concentration difference could be demonstrated for either peptide at rest. Although CWT caused a three-fold increase in the simultaneously measured renal venous overflow of noradrenaline, indicating markedly increased renal sympathetic nerve activity, the arterial levels of NPY-LI were unchanged and the net renal overflow of NPY-LI was only slightly (not significant) and transiently increased. CWT also failed to influence CGRP-LI levels in arterial plasma or CGRP overflow from the kidney. During ADR infusions, which increased arterial plasma ADR from 0.25 +/- 0.06 to 6.43 +/- 0.27 nmol l-1, NPY-LI levels were unaffected but CGRP-LI levels in arterial plasma were elevated (P less than 0.001 by ANOVA). No net renal overflow of CGRP-LI could, however, be demonstrated. These findings suggest that CWT is not powerful enough a stimulus to significantly enhance systemic or renal overflows of NPY-LI even though marked renal sympathetic activation occurs. During ADR infusions plasma CGRP-LI levels increased but renal veno-arterial differences of CGRP-LI were unaltered, indicating that CGRP-LI may be released from sites other than the kidney by ADR.
authors.affiliation: Department of Clinical physiology, Karolinska Hospital, Stockholm, Sweden.
authors.forename: B
authors.initials: B
authors.lastname: Tidgren
authors.forename: E
authors.initials: E
authors.lastname: Theodorsson
authors.forename: P
authors.initials: P
authors.lastname: Hjemdahl
mesh_terms: D000328:Adult; D015740:Calcitonin Gene-Related Peptide / Q000097:blood*; D004837:Epinephrine / Q000494:pharmacology*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007262:Infusions, Intravenous; D007668:Kidney / Q000378:metabolism*; D008297:Male; D009478:Neuropeptide Y / Q000097:blood*; D009638:Norepinephrine / Q000097:blood; D012079:Renal Circulation; D012082:Renal Veins; D013315:Stress, Psychological / Q000097:blood*
chemical_list: D009478:Neuropeptide Y; D015740:Calcitonin Gene-Related Peptide; D009638:Norepinephrine; D004837:Epinephrine