Coralline shape of the bacterial nucleoid after cryofixation.
A new procedure of immunostaining sections of cryofixed and freeze-substituted Escherichia coli shows that DNA extends from its bulk into small ribosome-free spaces throughout the cytoplasm, resulting in a coralline-shaped nucleoid. Low-resolution imaging of a bacterium reconstructed from serial sections demonstrated that the small excrescencies are not resolved. The resulting photograph shows the same features as phase-contrast light micrographs.
Bohrmann
B
B
Department of Microbiology, University of Basel, Switzerland.
Villiger
W
W
Johansen
R
R
Kellenberger
E
E
D002843:Chromatin / Q000648:ultrastructure; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000648:ultrastructure*; D005404:Fixatives; D007150:Immunohistochemistry; D007158:Immunologic Techniques; D008854:Microscopy, Electron
D002843:Chromatin; D004269:DNA, Bacterial; D005404:Fixatives