Ki-67 and B72.3 expression in breast cancer: an immunohistochemical study.
The present study was performed to evaluate Ki-67 and B72.3 immunostaining in 20 selected cases of breast cancer. In particular, we have examined the intracellular localization of TAG 72 and the tumour growth fraction, identified by Ki-67 antibody, on frozen sections of mammary carcinoma, by immunohistochemical technique (ABC method sec.Hsu). Immunostaining of TAG 72 and Ki-67 antigen was related to histologic subtype, diameter, nodal involvement, and number of positive axillary nodes. The preliminary results suggest that: (a) the presence of Ki-67 nuclear staining appeared to be associated with a poorer degree of differentiation, but no direct relationships were observed with diameter and nodal involvement; (b) no correlation between Ki-67 labelling rates and B72.3 intracytoplasmic immunostaining was observed; (c) myoepithelial cells show weak intracytoplasmic positivities.
Carriero
M V
MV
Department of Pathology, National Cancer Institute of Naples, Italy.
Bevilacqua
A M
AM
Borrelli
A
A
Di Bonito
M
M
Tatangelo
F
F
Apicella
A
A
D000328:Adult; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D001943:Breast Neoplasms / Q000378:metabolism*; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D019394:Ki-67 Antigen; D008875:Middle Aged
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D006023:Glycoproteins; D019394:Ki-67 Antigen; C047052:tumor-associated antigen 72