title: S-100-immunoreactive giant macrophages in lymphoid tissues of the guinea pig.
abstract: Giant cells containing S-100 protein of the lymphoid tissues in the guinea pig were studied by immunohistochemistry using S-100 antiserum. S-100-immunoreactive giant cells were dendritic in shape, contained one or two irregular-shaped, euchromatic nuclei, phagosomes of various diameter, numerous mitochondria and microfilaments in the perikaryon, and extended cell processes free of cell organelles. These cells predominantly lined the superficial cortex facing the subcapsular sinus, were less numerously scattered in the medulla of lymph nodes and located at the marginal zone of the spleen. They also stained with S-100 alpha monoclonal antiserum and showed active phagocytosis for aldehyde-fixed red cells or colloidal carbon in the popliteal lymph node and spleen. S-100-immunoreactive giant cells also appeared in the corticomedullary zone of the thymus and in the interfollicular area of the Peyer's patches of the gut. Small sinus macrophages, which exhibited active phagocytosis for colloidal carbon but were less active for red cells in the popliteal lymph node and spleen, were not stained with S-100 antiserum. These findings indicate that S-100-immunoreactive giant cells of the lymph node and spleen are a subpopulation of macrophages different from S-100-negative cells of the small type.
authors.affiliation: Department of Veterinary Anatomy, Faculty of Agriculture, Gifu University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Atoji
authors.forename: D
authors.initials: D
authors.lastname: Shirogane
authors.forename: T
authors.initials: T
authors.lastname: Kurono
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Sugimura
mesh_terms: D000818:Animals; D002965:Classification; D003713:Dendritic Cells / Q000737:chemistry / Q000166:cytology; D015726:Giant Cells / Q000737:chemistry / Q000166:cytology* / Q000502:physiology; D006168:Guinea Pigs; D007124:Immunoenzyme Techniques; D008198:Lymph Nodes / Q000166:cytology*; D010587:Phagocytosis / Q000502:physiology; D009418:S100 Proteins / Q000032:analysis*; D013154:Spleen / Q000166:cytology*
chemical_list: D009418:S100 Proteins