The major histocompatibility complex class II-linked cim locus controls the kinetics of intracellular transport of a classical class I molecule.
The dominant trans-acting major histocompatibility complex (MHC)-linked class I modifier (cim) locus, previously recognized through its ability to determine altered alloantigenicity of a rat class I molecule, RT1.A3, is shown here to influence class I intracellular transport. The MHC recombinant laboratory rat strains PVG.R1 and PVG.R8 display unusually long retention of RT1.Aa within the endoplasmic reticulum or cis-Golgi. In appropriate F1 hybrid cells heterozygous for RT1.Aa and another class I MHC allele, RT1.Ac, only the RT1.Aa protein is subject to slow transport. The cim gene product therefore shows class I allele specificity in its action, cim appears to be a polymorphic locus whose product is directly involved in the processes of class I MHC assembly and/or intracellular transport.
Powis
S J
SJ
Department of Immunology, Agricultural & Food Research Council, Institute of Animal Physiology & Genetics Research, Cambridge Research Station, Babraham, UK.
Howard
J C
JC
Butcher
G W
GW
D000818:Animals; D001692:Biological Transport; D002451:Cell Compartmentation; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism*; D008285:Major Histocompatibility Complex; D051379:Mice; D011499:Protein Processing, Post-Translational; D051381:Rats; D014162:Transfection
D015395:Histocompatibility Antigens Class I