In vivo formation of leukotriene E5 by murine peritoneal cells.
Resident mouse peritoneal cells, stimulated in vivo with opsonized zymosan, produced leukotriene C4 and E4, with LTE4 being the major (80-90%) product. When mice were placed on diets containing increasing amounts of fish oil, four additional sulfidopeptide leukotrienes (SP-LT), LTC5, LTE5, 11-trans LTC5 and 11-trans LTE5, were identified. The identity of LTE5 was confirmed by spectrophotometric, chromatographic and enzymatic methods. When equivalent amounts of n-6 and n-3 polyunsaturated fatty acids (PUFA) were included in the diet, the stimulated peritoneal cells (in vivo) produced higher quantities of LTE5 (30.2 +/- 5.4 ng/10(6) cells) than LTE4 (22.8 +/- 7.3 ng/10(6) cells). In addition, in vitro studies demonstrated a 60% reduction in LTC4 (42.0 +/- 10.8 ng/10(6) cells to 16.7 +/- 6.2 ng/10(6) cells) and the appearance of LTC5 (2.1 +/- 0.9 ng/10(6) cells) in resident macrophages (stimulated with A23187) from mice maintained on a fish oil diet compared to mice fed the control diet. This study demonstrated that formation of the pentaenyl SP-LT in vivo, in particular LTE5, by peritoneal cells can significantly contribute to the endogenous SP-LT pool in response to an inflammatory stimulus following a dietary regimen containing fish oil.
Whelan
J
J
Lipids Research Laboratory, Institute of Food Science, Cornell University, Ithaca, NY 14853.
Broughton
K S
KS
Lokesh
B
B
Kinsella
J E
JE
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004041:Dietary Fats / Q000494:pharmacology*; D005395:Fish Oils / Q000494:pharmacology*; D066298:In Vitro Techniques; D015289:Leukotrienes / Q000096:biosynthesis* / Q000302:isolation & purification; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D010529:Peritoneal Cavity; D012016:Reference Values; D015054:Zymosan / Q000494:pharmacology
D004041:Dietary Fats; D005395:Fish Oils; D015289:Leukotrienes; C068595:leukotriene E5; D015054:Zymosan