Autoantibodies to nucleolin in systemic lupus erythematosus and other diseases.
The 110-kDa intracellular phosphoprotein (110K) described previously by this laboratory as a common IgM autoantigen in SLE and certain other systemic autoimmune disorders and viral infections is identified as nucleolin in the present investigation. Using rabbit antiserum to rat nucleolin as a probe, IgM autoantibody-reactive 110K co-migrated with human lymphocyte nucleolin in one- and two-dimensional immunoblots. Rabbit anti-nucleolin also specifically depleted autoreactive 110K from detergent lysates of human cells. Because nucleolin shares amino acid sequence similarity and/or forms dynamic particles with other prominent autoantigens, the present observation raises the possibility that the nucleolin/anti-nucleolin system may be of special significance for the development of humoral autoreactivity to nuclear Ag.
Minota
S
S
Third Department of Internal Medicine, University of Tokyo, Japan.
Jarjour
W N
WN
Suzuki
N
N
Nojima
Y
Y
Roubey
R A
RA
Mimura
T
T
Yamada
A
A
Hosoya
T
T
Takaku
F
F
Winfield
J B
JB
D000918:Antibody Specificity; D001323:Autoantibodies / Q000276:immunology*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D007526:Isoelectric Point; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000737:chemistry / Q000276:immunology*; D010750:Phosphoproteins / Q000737:chemistry / Q000276:immunology*; D011233:Precipitin Tests; D016601:RNA-Binding Proteins; D000097796:Nucleolin
D001323:Autoantibodies; D007075:Immunoglobulin M; D009687:Nuclear Proteins; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D000097796:Nucleolin