title: Low density lipoprotein receptors on epithelial cell (Madin-Darby canine kidney) monolayers. Asymmetric distribution correlates with functional difference.
abstract: Low density lipoprotein (LDL) receptors are present on both the apical and basal surfaces of confluent monolayers of Madin-Darby canine kidney (MDCK) epithelial cells grown on gelatin-coated polycarbonate filters. Although there is only a single species of receptor protein present, as shown by immunoblotting, the receptors on the two surfaces were found to behave differently. LDL receptors on the basal surface show all of the characteristics of the LDL receptor described in fibroblasts in that their number is dependent upon the sterol (or LDL) content of the medium; however, regulation is only affected by LDL in the medium in contact with the basal side. In contrast, the apical surface LDL receptors are not regulated by the presence of LDL in the media on either the apical or basal surface. LDL particles can be transported across the monolayer in a temperature-dependent and -specific manner from the apical to the basal sides of the cell, but not in the opposite direction. The binding of 125I-LDL to both surfaces can be effectively inhibited not only by unlabeled LDL and very low density lipoprotein, but also by an antibody directed against the LDL receptor. The data suggest that the LDL receptors on the two aspects of the cell surface are biochemically identical, but differ in function. Thus, the basal surface receptor is involved in the control of cell cholesterol homeostasis, while that on the apical surface is responsible for the transport of LDL to the basal side.
authors.affiliation: Department of Physiology, University of Alberta, Edmonton, Canada.
authors.forename: C X
authors.initials: CX
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Stifani
authors.forename: W J
authors.initials: WJ
authors.lastname: Schneider
authors.forename: M J
authors.initials: MJ
authors.lastname: Poznansky
mesh_terms: D000818:Animals; D001692:Biological Transport; D015153:Blotting, Western; D002417:Cattle; D002451:Cell Compartmentation; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D003470:Culture Media; D004848:Epithelium / Q000378:metabolism* / Q000648:ultrastructure; D066298:In Vitro Techniques; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism*; D011973:Receptors, LDL / Q000276:immunology / Q000378:metabolism*; D013696:Temperature
chemical_list: D003470:Culture Media; D008077:Lipoproteins, LDL; D011973:Receptors, LDL; D002784:Cholesterol