title: A novel seed protein gene from Vicia faba is developmentally regulated in transgenic tobacco and Arabidopsis plants.
abstract: We have isolated a novel gene, denoted USP, from Vicia faba var. minor, which corresponds to the most abundant mRNA present in cotyledons during early seed development; however, the corresponding protein does not accumulate in cotyledons. The characterized USP gene with its two introns is 1 of about 15 members of a gene family. A fragment comprising 637 bp of 5' flanking sequence and the total 5' untranslated region was shown to be sufficient to drive the mainly seed-specific expression of two reporter genes, coding for neomycin phosphotransferase II and beta-glucuronidase, in transgenic Arabidopsis thaliana and Nicotiana tabacum plants. We showed that the USP promoter becomes active in transgenic tobacco seeds in both the embryo and the endosperm, whereas its activity in Arabidopsis is detectable only in the embryo. Moreover, we demonstrated a transient activity pattern of the USP promoter in root tips of both transgenic host species.
authors.affiliation: Zentralinstitut für Genetik, Kulturpflanzenforschung der AdW, Gatersleben, FRG.
authors.forename: H
authors.initials: H
authors.lastname: Bäumlein
authors.forename: W
authors.initials: W
authors.lastname: Boerjan
authors.forename: I
authors.initials: I
authors.lastname: Nagy
authors.forename: R
authors.initials: R
authors.lastname: Bassüner
authors.forename: M
authors.initials: M
authors.lastname: Van Montagu
authors.forename: D
authors.initials: D
authors.lastname: Inzé
authors.forename: U
authors.initials: U
authors.lastname: Wobus
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D007887:Fabaceae / Q000235:genetics*; D005786:Gene Expression Regulation; D017343:Genes, Plant; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D006651:Histocytochemistry; D007438:Introns; D008969:Molecular Sequence Data; D005810:Multigene Family; D010940:Plant Proteins / Q000235:genetics*; D010946:Plants, Medicinal; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012639:Seeds / Q000235:genetics*; D014026:Nicotiana / Q000166:cytology / Q000235:genetics* / Q000254:growth & development
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; D004247:DNA; D005966:Glucuronidase