title: Fast oxygen-independent degradation of the D1 reaction center protein in photosystem II.
abstract: The D1 reaction center protein in photosystem II is rapidly degraded during illumination of chloride-depleted or Tris-washed thylakoids. The degradation is independent of oxygen and occurs under anaerobic conditions provided that electrons can flow through the acceptor-side of photosystem II. This shows that oxygen-derived reactive species are not necessarily involved in the light-dependent damage of the D1 protein. Instead the illumination of chloride-depleted or Tris-washed thylakoids induces long-lived, strongly oxidizing radicals on the donor-side of photosystem II which are suggested to be the damaging species for the D1 protein.
authors.affiliation: Department of Biochemistry, Arrhenius Laboratories for Natural Sciences, Stockholm University, Sweden.
authors.forename: C
authors.initials: C
authors.lastname: Jegerschöld
authors.forename: S
authors.initials: S
authors.lastname: Styring
mesh_terms: D000332:Aerobiosis / Q000187:drug effects; D000693:Anaerobiosis / Q000187:drug effects; D002712:Chlorides / Q000494:pharmacology; D002736:Chloroplasts / Q000378:metabolism*; D004579:Electron Transport / Q000187:drug effects; D006868:Hydrolysis; D010100:Oxygen / Q000378:metabolism*; D010788:Photosynthesis; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism*; D045332:Photosystem II Protein Complex; D010944:Plants / Q000378:metabolism
chemical_list: D002712:Chlorides; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010100:Oxygen