Phagocytic cell chemiluminescence using different zymosan preparations.
This study compared the effectiveness of opsonized and unopsonized zymosan prepared in our laboratories with a commercially available opsonized preparation used for induction of luminol-dependent oxidative burst in phagocytic cells. The production of chemiluminescence (CL) by human whole blood, isolated human neutrophils, normal BALB c mouse splenocytes, and an immortal BALB c mouse macrophage cell line (J774A.1) was tested in an automated luminometer. Recombinant murine or human interferon-gamma (IFN-gamma) and lipopolysaccharide (LPS) were used as priming agents in some of the experiments. With human leukocytes and normal mouse spleen cells the laboratory-prepared zymosans (regardless of opsonization) induced equal or significantly greater CL than did the commercially prepared zymosan. In addition, greatly increased CL was evident with IFN-gamma- and LPS-primed neutrophils tested with our zymosans compared with the commercial preparation. These results suggest that effective zymosans capable of inducing strong, reproducible CL responses from several different phagocytic cell populations can be readily made in the laboratory.
Gridley
D S
DS
Department of Microbiology, Loma Linda University School of Medicine, California 92350.
Lau
B H
BH
Tosk
J M
JM
D000818:Animals; D002460:Cell Line; D006801:Humans; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009895:Opsonin Proteins; D010100:Oxygen / Q000378:metabolism; D010586:Phagocytes / Q000378:metabolism*; D015054:Zymosan / Q000302:isolation & purification
D009895:Opsonin Proteins; D015054:Zymosan; D010100:Oxygen