title: Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1.
abstract: By using a potential-dependent J-aggregate-forming delocalized lipophilic cation, 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine++ + iodide (JC-1), we find that membrane potentials across mitochondria in a living cell can be heterogeneous. Remarkably, even within a long contiguous mitochondrion, regional heterogeneity in membrane potentials appears to be possible.
authors.affiliation: Dana-Farber Cancer Institute, Boston, MA.
authors.forename: S T
authors.initials: ST
authors.lastname: Smiley
authors.forename: M
authors.initials: M
authors.lastname: Reers
authors.forename: C
authors.initials: C
authors.lastname: Mottola-Hartshorn
authors.forename: M
authors.initials: M
authors.lastname: Lin
authors.forename: A
authors.initials: A
authors.lastname: Chen
authors.forename: T W
authors.initials: TW
authors.lastname: Smith
authors.forename: G D
authors.initials: GD
authors.lastname: Steele
authors.forename: L B
authors.initials: LB
authors.lastname: Chen
mesh_terms: D000818:Animals; D001562:Benzimidazoles / Q000737:chemistry*; D002232:Carbocyanines / Q000737:chemistry*; D002412:Cations; D002460:Cell Line; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D006224:Cricetinae; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000502:physiology; D008055:Lipids / Q000737:chemistry; D008564:Membrane Potentials; D051379:Mice; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000502:physiology*; D012995:Solubility; D013050:Spectrometry, Fluorescence
chemical_list: D001562:Benzimidazoles; D002232:Carbocyanines; D002412:Cations; D005456:Fluorescent Dyes; D008055:Lipids; C068624:5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine