Early aspects of Caenorhabditis elegans sex determination and dosage compensation are regulated by a zinc-finger protein.
The sdc-1 gene acts at an early step in the regulatory hierarchy that controls the choice of sexual fate in Caenorhabditis elegans. It functions at a point before the control of sex determination and X-chromosome dosage compensation diverge. Here we report that sdc-1 encodes a protein of 1,203 amino acids containing seven zinc fingers. This protein motif in combination with other genetic and molecular information suggests that sdc-1 is likely to function as an embryonic transcription factor regulating downstream genes involved specifically in the sex determination and dosage compensation pathways, or regulating other genes involved in the coordinate control of both processes. These results enhance our general understanding of sex determination strategies, which are already known to involve transcriptional regulation and alternative RNA splicing in Drosophila melanogaster, DNA rearrangements in Saccharomyces cerevisiae, and transcriptional regulation in mammals.
Nonet
M L
ML
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Meyer
B J
BJ
D000595:Amino Acid Sequence; D000818:Animals; D002107:Caenorhabditis / Q000235:genetics* / Q000502:physiology; D005260:Female; D015723:Gene Library; D007438:Introns; D008297:Male; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D012732:Sex Determination Analysis; D014158:Transcription, Genetic; D016335:Zinc Fingers / Q000235:genetics*
D015345:Oligonucleotide Probes