Screening infertile women for the assessment of corpus luteal function and their response to therapy by ELISA of pregnanediol glucuronide.
Corpus luteal function was assessed by estimating pregnanediol 3-alpha-glucuronide (PdG) in three midluteal-phase urine samples collected from 85 women attending the infertility clinic. The previously established cut off limits based on PdG estimations were useful in detecting anovulation in 23 cases, corpus luteal adequacy in 42 cases and corpus luteum deficiency (CLD) in 20 cases. In 8 women CLD could be corrected with 50 mg of clomiphene citrate (CC) therapy whereas 6 women required 100 mg of CC and 3 pregnancies were achieved. This rapid screening method is thus useful in segregating a large number of women according to their ovulatory status and in the subsequent treatment of CLD.
Khatkhatay
I
I
Endocrinology and Infertility Clinic, Institute for Research in Reproduction (ICMR), Parel, Bombay, India.
Desai
M P
MP
Sankolli
G M
GM
Meherji
P K
PK
Joshi
U M
UM
D002996:Clomiphene / Q000494:pharmacology; D003338:Corpus Luteum / Q000187:drug effects / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000628:therapy*; D008297:Male; D010061:Ovulation Detection; D011276:Pregnanediol / Q000031:analogs & derivatives* / Q000652:urine
C022270:pregnanediol-3 alpha-glucuronide; D002996:Clomiphene; D011276:Pregnanediol