title: Up regulation of calbindin-D28K mRNA in the rat hippocampus following focal stimulation of the perforant path.
abstract: Calbindin-D28K is a constitutive Ca2(+)-binding protein expressed in hippocampal neurons that are resistant to various forms of excitotoxic injury. However, the local factors controlling calbindin-D28K expression within the central nervous system are unknown. We report that neuronal excitation via the perforant path leads to an increased expression of calbindin-D28K mRNA within dentate granule cells. This response is related specifically to stimulation that induces prolonged periods of bursting afterdischarges and precedes cellular injury. The up regulation of calbindin-D28K mRNA occurs during the type of neuronal activation associated with elevated cytosolic Ca2+ and suggests that the maintenance of Ca2+ homeostasis includes a system of feedback control at the level of gene expression.
authors.affiliation: Department of Neurology, University of California, San Francisco 94143.
authors.forename: D H
authors.initials: DH
authors.lastname: Lowenstein
authors.forename: M F
authors.initials: MF
authors.lastname: Miles
authors.forename: F
authors.initials: F
authors.lastname: Hatam
authors.forename: T
authors.initials: T
authors.lastname: McCabe
mesh_terms: D000818:Animals; D064092:Calbindin 1; D064026:Calbindins; D003345:Corticosterone / Q000097:blood; D004558:Electric Stimulation; D006098:Granulocytes / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D064030:S100 Calcium Binding Protein G / Q000235:genetics*; D015854:Up-Regulation
chemical_list: C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D064030:S100 Calcium Binding Protein G; D003345:Corticosterone