title: The crystal structure of silver carp insulin at medium resolution.
abstract: It is an important way of surveying the structure-function relationship of insulin to study insulins from different species. Based on the structure model of an orthorhombic crystal obtained by the molecular replacement method, the crystallographic refinement of a hexamer of silver carp insulin in an asymmetric unit has been carried out with 2.8 A resolution data using the restrained least-squares method. The comparisons of insulin structures have shown that the six silver carp insulin molecules have very similar but not identical three-dimensional structures which are similar to the known 2 Zn pig insulin structure but remarkably different in some local conformations.
authors.affiliation: Shanghai Institute of Biochemistry, Academia Sinica, PRC.
authors.forename: Z W
authors.initials: ZW
authors.lastname: Li
authors.forename: R C
authors.initials: RC
authors.lastname: Bi
authors.forename: B G
authors.initials: BG
authors.lastname: Zhao
authors.forename: Y W
authors.initials: YW
authors.lastname: Yan
authors.forename: J K
authors.initials: JK
authors.lastname: Fang
mesh_terms: D000818:Animals; D002347:Carps; D003460:Crystallization; D007328:Insulin / Q000737:chemistry*; D008968:Molecular Conformation; D013329:Structure-Activity Relationship
chemical_list: D007328:Insulin