title: Estrogenic action of estriol fatty acid esters.
abstract: Recent studies suggest that, estriol, like estradiol, is biosynthetically esterified with fatty acids. We have synthesized the stearate estriol, at C-16 alpha, C-17 beta and the diester, C-16 alpha,17 beta and tested these D-ring esters for their estrogenic action both in vivo and in vitro, comparing them to estradiol, estriol and estradiol-17-stearate. None of the estriol esters bind to the estrogen receptor. They are only weakly estrogenic in a microtiter plate estrogen bioassay: stimulation of alkaline phosphatase in the Ishikawa endometrial cells. However, both estriol monoesters are extremely potent estrogens when injected subcutaneously (in aqueous alcohol) into ovariectomized mice. Compared to the free steroids, they produced a dramatically increased uterine weight with a greatly prolonged duration of stimulation. The 16 alpha,17 beta-diester also induced a protracted uterotrophic response, but the stimulation of uterine weight was comparatively low. Since the esters of estradiol and estriol do not bind to the estrogen receptor, their estrogenic signal must be generated through the action of esterase enzymes. These naturally occurring esters have the potential of being extremely useful pharmacological agents for long-lived estrogenic stimulation.
authors.affiliation: Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, CT 06510.
authors.forename: J E
authors.initials: JE
authors.lastname: Zielinski
authors.forename: S L
authors.initials: SL
authors.lastname: Pahuja
authors.forename: J M
authors.initials: JM
authors.lastname: Larner
authors.forename: R B
authors.initials: RB
authors.lastname: Hochberg
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive; D004952:Esters / Q000378:metabolism / Q000494:pharmacology*; D004964:Estriol / Q000378:metabolism / Q000494:pharmacology*; D004967:Estrogens / Q000378:metabolism / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D051379:Mice; D010052:Ovariectomy; D011960:Receptors, Estrogen / Q000378:metabolism; D013228:Stearates / Q000494:pharmacology; D014599:Uterus / Q000187:drug effects
chemical_list: D004952:Esters; D004967:Estrogens; D005227:Fatty Acids; D011960:Receptors, Estrogen; D013228:Stearates; D000469:Alkaline Phosphatase; D004964:Estriol