Chromosomal rearrangement in Candida stellatoidea results in a positive effect on phenotype.
When type I Candida stellatoidea is plated onto sucrose agar at levels in excess of 10(8) cells, some isolates spontaneously form sucrose-positive colonies. These isolates do not display typical type I phenotypes but instead exhibit phenotypes intermediate between type I C. stellatoidea and C. albicans. Also, this phenotypic change only occurs in conjunction with a chromosomal rearrangement. These rearrangements have been studied in a strain naturally marked for methionine auxotrophy. Chromosome-size DNA bands separated by pulsed-field gel electrophoresis were probed with genes cloned from C. albicans. The hybridization pattern indicated that the genes on several chromosomes underwent extensive rearrangement.
Wickes
B L
BL
Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Golin
J E
JE
Kwon-Chung
K J
KJ
D002175:Candida / Q000235:genetics*; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000032:analysis; D015321:Gene Rearrangement; D007621:Karyotyping; D010641:Phenotype; D011003:Ploidies; D013395:Sucrose / Q000378:metabolism
D004271:DNA, Fungal; D013395:Sucrose