title: Guidelines for the preparation and analysis of the fragile X chromosome in lymphocytes.
abstract: The following guidelines were adopted by an Ad Hoc Committee convened at the Fourth International Workshop on the Fragile X Syndrome and X-Linked Mental Retardation to establish minimum cytogenetic standards for the preparation and analysis of the fragile X chromosome. The intention of the committee was to develop and provide practical standards for the routine cytogenetic detection of the fragile X. The guidelines describe reasonable criteria for effective tissue culture methods for eliciting the Xq27.3 fragile site in vitro and for the analysis of such chromosome preparations.
authors.affiliation: Department of Pathology/Cytogenetics, Kaiser-Permanente, Portland, Oregon.
authors.forename: P B
authors.initials: PB
authors.lastname: Jacky
authors.forename: Y R
authors.initials: YR
authors.lastname: Ahuja
authors.forename: K
authors.initials: K
authors.lastname: Anyane-Yeboa
authors.forename: W R
authors.initials: WR
authors.lastname: Breg
authors.forename: N J
authors.initials: NJ
authors.lastname: Carpenter
authors.forename: U G
authors.initials: UG
authors.lastname: Froster-Iskenius
authors.forename: J P
authors.initials: JP
authors.lastname: Fryns
authors.forename: T W
authors.initials: TW
authors.lastname: Glover
authors.forename: K H
authors.initials: KH
authors.lastname: Gustavson
authors.forename: S F
authors.initials: SF
authors.lastname: Hoegerman
mesh_terms: D002478:Cells, Cultured; D002871:Chromosome Banding; D003470:Culture Media / Q000494:pharmacology; D005260:Female; D005492:Folic Acid / Q000494:pharmacology; D005600:Fragile X Syndrome / Q000235:genetics* / Q000473:pathology; D005821:Genetic Techniques; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure*; D008297:Male; D012494:Sampling Studies; D013048:Specimen Handling; D009626:Terminology as Topic; D013936:Thymidine / Q000494:pharmacology; D014960:X Chromosome / Q000187:drug effects / Q000648:ultrastructure*
chemical_list: D003470:Culture Media; D005492:Folic Acid; D013936:Thymidine