title: Role of antibody to extracellular proteins of Rhodococcus equi in protection against R. equi pneumonia in foals.
abstract: Rhodococcus equi produces two exoenzymes (REE), a cholesterol oxidase in large amounts and a phospholipase C, which cause lysis of sheep red blood cells (SRBC) sensitized with Staphylococcus aureus beta toxin. Two immunization studies were done in foals to determine the role of antibody to REE in protection against R. equi pneumonia. In the first study, three foals (mean age 10 days) were vaccinated four times at 2-week intervals with over 1 million units of partially purified exoenzymes (PREE). In the second study, three foals (mean age 19 days) were administered plasma from an adult horse vaccinated with PREE. Relatively low titres (16-32) of neutralizing antibody were detected in the foals of the former group, and passive transfer of neutralizing antibody (titres 32-64) occurred in the latter. Following immunization, principal foals and an equal number of similarly aged nonimmunized foals were challenged by aerosol with 1 x 10(10) live R. equi per day for 5 consecutive days. No severe clinical pneumonia developed in either group and, with one exception, only minor and resolving lung abscesses developed in these foals. These studies showed that antibody response of foals to immunization with PREE was poor, antibody to PREE did not prevent foals from developing lung abscesses following experimental infection, and that foals even as young as 3 weeks of age may be largely refractory to aerosol challenge with virulent R. equi.
authors.affiliation: Department of Veterinary Microbiology and Immunology, Ontario Veterinary College, University of Guelph, Canada.
authors.forename: R S
authors.initials: RS
authors.lastname: Machang'u
authors.forename: J F
authors.initials: JF
authors.lastname: Prescott
mesh_terms: D000193:Actinomycetales Infections / Q000517:prevention & control / Q000662:veterinary*; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000276:immunology / Q000302:isolation & purification; D001428:Bacterial Vaccines / Q000276:immunology; D002789:Cholesterol Oxidase / Q000276:immunology / Q000302:isolation & purification; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006734:Horse Diseases / Q000517:prevention & control*; D006736:Horses; D007116:Immunization, Passive; D008168:Lung / Q000473:pathology; D011014:Pneumonia / Q000517:prevention & control / Q000662:veterinary*; D012240:Rhodococcus / Q000276:immunology*; D010738:Type C Phospholipases / Q000276:immunology / Q000302:isolation & purification; D014611:Vaccination / Q000662:veterinary
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D002789:Cholesterol Oxidase; D010738:Type C Phospholipases