Determination of N-acetylcysteine in human plasma by gas chromatography-mass spectrometry.
An automatic mass spectrometric method for the quantitation of N-acetylcysteine (NAC) in human plasma has been developed. NAC was extracted from plasma with ethyl acetate and derivatized in two steps with 2-propanol and pentafluoropropionic anhydride. The volatile derivative obtained was ideal for gas chromatographic-mass spectrometric analysis. Data obtained by analysing the plasma of healthy volunteers to whom 600 mg of NAC had been orally given are reported.
Longo
A
A
Zambon Research S.p.A., Milan, Italy.
Di Toro
M
M
Galimberti
C
C
Carenzi
A
A
D000111:Acetylcysteine / Q000097:blood*; D001322:Autoanalysis; D003545:Cysteine / Q000031:analogs & derivatives / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D006207:Half-Life; D006801:Humans; D007202:Indicators and Reagents
D007202:Indicators and Reagents; C068765:N-propionylcysteine; D003545:Cysteine; D000111:Acetylcysteine