title: Characterization of phosphopeptide derived from bovine beta-casein: an inhibitor to intra-intestinal precipitation of calcium phosphate.
abstract: Casein phosphopeptide (CPP), a highly phosphorylated peptide fragment which inhibits the formation of hydroxyapatite crystal was isolated from pooled ileal contents of rats fed a semi-synthetic diet containing bovine beta-casein. The estimated amino acid sequence of the CPP was shorter than that of the trypsin-digested beta-casein but the core region consisting of consecutive bindings of phosphoserine was fully conserved. A moderate and exchangeable binding to Ca2+ of the CPP molecule well substantiates the high absorbability of calcium from milk and dairy products.
authors.affiliation: Department of Agricultural Chemistry, University of Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Sato
authors.forename: M
authors.initials: M
authors.lastname: Shindo
authors.forename: H
authors.initials: H
authors.lastname: Gunshin
authors.forename: T
authors.initials: T
authors.lastname: Noguchi
authors.forename: H
authors.initials: H
authors.lastname: Naito
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002130:Calcium Phosphates / Q000378:metabolism*; D002364:Caseins / Q000032:analysis*; D002417:Cattle; D011232:Chemical Precipitation; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis*; D010748:Phosphopeptides / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000596:Amino Acids; D002130:Calcium Phosphates; D002364:Caseins; D010446:Peptide Fragments; D010748:Phosphopeptides; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous