Evaluation of the fiber-optic antibody-based fluoroimmunosensor for DNA adducts in human placenta samples.
This paper presents the application of a fiber-optic antibody-based fluoroimmunosensor (FIS) for measuring DNA adducts of benzo[alpha]pyrene in human placenta samples. The FIS combines the excellent specificity of the antigen-antibody reaction, the high sensitivity of laser excitation, and the versatility of fiber-optic technology. The FIS was used to detect benzo[alpha]pyrene tetrol (BPT) after release from DNA by mild acid hydrolysis. These placenta samples contained one molecule of adduct per 10(7) base pairs. The limit of detection of the FIS for BPT is about 14 amol (14 x 10(-18) mol).
Vo-Dinh
T
T
Health and Safety Research Division, Oak Ridge National Laboratory, TN 37831-6101.
Alarie
J P
JP
Johnson
R W
RW
Sepaniak
M J
MJ
Santella
R M
RM
D001564:Benzo(a)pyrene / Q000737:chemistry* / Q000633:toxicity; D004247:DNA / Q000737:chemistry* / Q000187:drug effects; D004781:Environmental Exposure; D005069:Evaluation Studies as Topic; D005260:Female; D005336:Fiber Optic Technology; D015200:Fluoroimmunoassay / Q000295:instrumentation / Q000379:methods; D006801:Humans; D055100:Optical Fibers; D010920:Placenta / Q000737:chemistry* / Q000187:drug effects
D001564:Benzo(a)pyrene; D004247:DNA