The effects of alcoholism and smoking on platelet eicosanoid production in vitro.
Ethanol induces changes in eicosanoid synthesis in blood platelets and brain tissue. Cigarette smoking also causes alterations in eicosanoid formation. This preliminary report examined in vitro platelet sonicate eicosanoid production using 14C-arachidonic acid (14C-AA) and in separate experiments, 14C-PGH2, as substrates. Radiometric thin layer chromatography (TLC) was used to identify the products formed. Eicosanoid product formation in platelet sonicates collected from 28 abstinent male alcoholics were compared to those from 11 male control subjects. All but one of the alcoholics were chronic smokers and all control subjects were non-smokers. All smokers abstained from smoking for 12 h prior to the blood collection to control for any acute effects of cigarette smoke on eicosanoid production. Significant reductions in platelet sonicate production of PGD2 and PGE2 in vitro were observed in alcoholic smokers when 14C-PGH2, but not 14C-AA, was the substrate. These reductions were predicted equally well by two variables, smoking and alcoholism, using several statistical models. This is the first investigation that controlled for the acute effects of smoking and accounted for the potential effects of cigarette smoking on platelet eicosanoid production in alcoholics. Because cigarette smoking is prevalent among alcoholics, future studies on the role of eicosanoids in alcoholism should control for smoking.
Burch
E A
EA
Veterans Administration Medical Center, New Orleans, LA.
Kadowitz
P J
PJ
Kotler-Cope
S
S
McNamara
D B
DB
D000437:Alcoholism / Q000378:metabolism*; D001792:Blood Platelets / Q000378:metabolism*; D001921:Brain / Q000378:metabolism; D002855:Chromatography, Thin Layer; D015232:Dinoprostone / Q000378:metabolism; D015777:Eicosanoids / Q000096:biosynthesis*; D000431:Ethanol / Q000009:adverse effects; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008954:Models, Biological; D015230:Prostaglandin D2 / Q000378:metabolism; D011450:Prostaglandin Endoperoxides, Synthetic / Q000378:metabolism; D044262:Prostaglandin H2; D011463:Prostaglandins H / Q000378:metabolism; D012907:Smoking / Q000378:metabolism*
D015777:Eicosanoids; D011450:Prostaglandin Endoperoxides, Synthetic; D011463:Prostaglandins H; D000431:Ethanol; D044262:Prostaglandin H2; D015232:Dinoprostone; D015230:Prostaglandin D2