Nucleotide sequence and taxonomic value of the major outer membrane protein gene of Chlamydia pneumoniae IOL-207.
Chlamydia pneumoniae IOL-207 genomic DNA was hybridized with a 1.5 kb labelled DNA probe containing the 3' region of the coding sequence for the major outer membrane protein (MOMP) of C. trachomatis serovar L1. An 8.5 kb Bg/II fragment containing the complete MOMP gene was cloned into lambda EMBL3. Two hybridizing EcoRI fragments were sub-cloned into the lambda ZAP II cloning vector and the resulting plasmids were used as templates for sequencing both strands of the C. pneumoniae MOMP gene. Computer taxonomic studies using the nucleotide and inferred amino acid sequence of the MOMP of C. pneumoniae IOL-207 and all known chlamydial MOMP sequences supported the designation of C. pneumoniae as a new species, but electron microscope studies suggested that the presence of pear-shaped elementary bodies (EBs) may not be a reliable taxonomic criterion.
Carter
M W
MW
Department of Microbiology, University of Southampton, Faculty of Medicine, Southampton General Hospital, UK.
al-Mahdawi
S A
SA
Giles
I G
IG
Treharne
J D
JD
Ward
M E
ME
Clark
I N
IN
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001483:Base Sequence; D005075:Biological Evolution; D015139:Blotting, Southern; D002689:Chlamydia / Q000145:classification / Q000235:genetics* / Q000648:ultrastructure; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005091:Exons; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010802:Phylogeny; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial