The phosphoenolpyruvate carboxylase gene family of Sorghum: promoter structures, amino acid sequences and expression of genes.
Two different members of the phosphoenolpyruvate carboxylase(PEPC)-encoding multigene family (clones lambda CP21 and lambda CP46) have been isolated from a Sorghum vulgare lambda EMBL4 genomic library. The use of the 3'-noncoding regions to probe Northern blots of RNA from roots, etiolated leaves and green leaves indicated that lambda CP21 and lambda CP46 encode the C3- and C4-type leaf PEPC isoforms, respectively. The lambda CP21 clone is expressed in the three tissues and is not light-regulated, whereas lambda CP46 is only expressed in greening leaves. The nucleotide sequence of the 5'-flanking DNA (520 bp) has been determined for both genes. For lambda CP46, several direct repeats were located in this region with similarities to sequences found in other light-regulated genes, but not in lambda CP21. The deduced amino acid sequences of the two S. vulgare PEPC proteins are 75% identical.
Crétin
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Laboratoire de Physiologie Végétale Moléculaire. Unité Associée CNRS-URA D1128, Université Paris-Sud. Orsay, France.
Santi
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S
Keryer
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Lepiniec
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L
Tagu
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Vidal
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Gadal
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P
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005091:Exons; D015870:Gene Expression; D017343:Genes, Plant; D015698:Genomic Library; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010730:Phosphoenolpyruvate Carboxylase / Q000235:genetics*; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D007527:Isoenzymes; D010730:Phosphoenolpyruvate Carboxylase