Nonradioactive in situ hybridization. A rapid approach for the identification of marker chromosomes: study of a case of acute leukemia with a Yq specific DNA probe.
Nonradioactive in situ hybridization provides a rapid method for detecting specific nucleic acid sequences. In this study of a patient with acute leukemia, we applied in situ hybridization for identification of a marker chromosome and determination of the number of copies of this marker in interphase nuclei using a biotinylated Yq-specific DNA probe (pY3.4). We show that nonradioactive interphase in situ hybridization can be a useful method for karyotypic analysis in addition to routine cytogenetic techniques in neoplastic disorders.
Wullich
B
B
Cancer Center of Southwest Biomedical Research Institute and Genetrix, Inc., Scottsdale, AZ 85251.
Morgan
R
R
Berger
C
C
Jarzabek
V
V
Sandberg
A A
AA
D000208:Acute Disease; D000368:Aged; D002871:Chromosome Banding; D015342:DNA Probes; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D007938:Leukemia / Q000235:genetics*; D008297:Male; D008677:Metaphase; D009693:Nucleic Acid Hybridization; D014998:Y Chromosome
D015342:DNA Probes; D005819:Genetic Markers