title: Quantitative cellular cytotoxicity to Brugia malayi microfilariae: in vitro studies of sera from an area endemic for filariasis in Andhra Pradesh, India.
abstract: The extent of eosinophil and neutrophil cell-mediated cytotoxicity on Brugia malayi microfilariae by sera from an area endemic for bancroftian filariasis in Andhra Pradesh, India, has been studied in vitro in terms of the clinical status of the subjects. At physical examination, 66 serum samples were collected. Group "A" included patients with various disease manifestations like lymphoedema, hydrocoele, lymphangitis and elephantiasis. Group "B" had microfilaraemia ranging from 1-300/ml of blood. Subjects with no history of infection past or present (endemic normals) were studied as Group "C". Out of 38 sera tested individually with eosinophils, 14/18 of Group A, 10/11 of Group B and 7/9 of Group C promoted higher (21-97%), moderate (18-88%) and highest (51-95%) range of cytotoxicity, respectively. The age, clinical status and duration of disease among the infected subjects appeared to correlate with the microfilarial mortality. In Group B, we observed the highest microfilarial count (16-300/ml) in lower (1-20 yrs) age groups. These individuals promoted higher (77-83%) cytotoxicity compared to the older age group (21-40 or 41-60) with low (1-36/ml) microfilaraemia. Group C sera were highly toxic to microfilariae. All those that were positive promoted greater than 50% mortality. Eighteen nonendemic normal sera had no effect on microfilariae. The overlapping but differential toxicity of the sera indicates that various clinical manifestations are associated with different types of cellular and humoral responses. These studies should help focus identification of the target epitopes of various immune responses of the host.
authors.affiliation: Department of Zoology, Andhra University, Waltair, India.
authors.forename: J
authors.initials: J
authors.lastname: Thommandru
authors.forename: G I
authors.initials: GI
authors.lastname: Higashi
authors.forename: S
authors.initials: S
authors.lastname: Santhanam
authors.forename: A
authors.initials: A
authors.lastname: Malhotra
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000818:Animals; D002009:Brugia / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D003602:Cytotoxicity, Immunologic; D004605:Elephantiasis, Filarial / Q000276:immunology*; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D007194:India; D007223:Infant; D008297:Male; D008842:Microfilariae / Q000276:immunology; D008875:Middle Aged