Multicentre comparison of the Novo Biolabs immunometric CK-MB assay with four established methods.
The analytical performance of a new immunometric CK-MB assay from Novo Biolabs was evaluated in three hospitals. Comparisons were made with another immunometric assay, an immune inhibition/precipitation technique, an immune inhibition method, and a column separation method. The Novo assay exhibited good precision (CV 3-7%) at all concentration levels tested (6-150 micrograms/L) and was found to be linear over the range 0-200 micrograms/L. Comparison of the Novo kit with the other methods using patients' sera showed acceptable correlation between the methods over a wide concentration range (r = 0.96-0.99). We conclude that the assay is a useful adjunct to the specific measurement of CK-MB, particularly in routine laboratories with a high throughput of such determinations.
Wulkan
R W
RW
Department of Clinical Chemistry, Academic Hospital Rotterdam-Dijkzigt, The Netherlands.
van Loon
J
J
Sturk
A
A
Boerma
G J
GJ
Verdegaal
M C
MC
van den Berg
B
B
Souverijn
J H
JH
Sanders
G T
GT
D003402:Creatine Kinase; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007231:Infant, Newborn; D007527:Isoenzymes; D015337:Multicenter Studies as Topic; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results
D007527:Isoenzymes; D011933:Reagent Kits, Diagnostic; D003402:Creatine Kinase