title: Neonatal seminal vesicle mesenchyme induces a new morphological and functional phenotype in the epithelia of adult ureter and ductus deferens.
abstract: Mesenchyme from neonatal mouse and rat seminal vesicles (SVM) was grown in association with postnatal (adult) epithelial cells from the ureter (URE) and ductus deferens (DDE) in chimeric tissue recombinants composed of mouse mesenchyme and rat epithelium or vice versa. Functional cytodifferentiation was examined in these SVM + URE and SVM + DDE tissue recombinants with antibodies against major androgen-dependent seminal-vesicle-specific secretory proteins. Adult DDE and URE were induced to express seminal cytodifferentiation and produced the complete spectrum of major seminal vesicle secretory (SVS) proteins. The SVS proteins produced were specific for the species that provided the epithelium. In the case of SVM + URE recombinants, the URE, which normally lacks androgen receptors (AR), expressed AR. These results demonstrate that adult epithelial cells retain a developmental plasticity equivalent to their undifferentiated fetal counterparts and are capable of being reprogrammed to express a completely new morphological, biochemical and functional phenotype.
authors.affiliation: Department of Anatomy, University of California, San Francisco 94143.
authors.forename: G R
authors.initials: GR
authors.lastname: Cunha
authors.forename: P
authors.initials: P
authors.lastname: Young
authors.forename: S J
authors.initials: SJ
authors.lastname: Higgins
authors.forename: P S
authors.initials: PS
authors.lastname: Cooke
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development* / Q000502:physiology; D002454:Cell Differentiation; D002678:Chimera; D004848:Epithelium / Q000502:physiology; D008297:Male; D008648:Mesoderm / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D011506:Proteins / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D011944:Receptors, Androgen / Q000502:physiology; D012669:Seminal Vesicles / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology; D014513:Ureter / Q000254:growth & development* / Q000502:physiology; D014649:Vas Deferens / Q000254:growth & development* / Q000502:physiology
chemical_list: D011506:Proteins; D011944:Receptors, Androgen