Estradiol-17 beta induces polyaromatic hydrocarbon-inducible cytochrome P-450 in chicken liver.
A cDNA clone has been isolated from a chicken liver library prepared against messenger RNA isolated after chronic estradiol - 17 beta treatment. The clone, pP-450 IA - 61, has an insert of 900nt and the sequence shows high homology to CYPIA2 subfamily from four other species. A single injection of estradiol - 17 beta to immature chicken results in a striking induction of mRNA hybridizing to labeled pP-450IA - 61. The probe also hybridizes to mRNA induced by 3 - methylcholanthrene in chicken. These results offer direct proof for the similarity in the mode of action at the transcriptional level of polyaromatic hydrocarbons and estrogenic compounds.
Murti
J R
JR
Department of Biochemistry, Indian Institute of Science, Bangalore.
Adiga
P R
PR
Padmanaban
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D003513:Cycloheximide / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000235:genetics*; D004790:Enzyme Induction; D004958:Estradiol / Q000494:pharmacology*; D015723:Gene Library; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology*; D051379:Mice; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D004958:Estradiol; D008748:Methylcholanthrene; D003577:Cytochrome P-450 Enzyme System; D003513:Cycloheximide