title: Purification and immunological characterization of a GroEL-like protein from Bordetella pertussis.
abstract: A GroEL-like protein from Bordetella pertussis was purified. This protein was found to have the tetradecameric subunit structure typical of the GroEL family of proteins and to contain epitopes similar to those of other members of this family, including a human GroEL-like protein. Active immunization of neonatal mice with the B. pertussis GroEL-like protein provided little protection against an aerosol challenge with B. pertussis. Antibodies to this protein were elicited in mice by a standard diphtheria-tetanus-pertussis (DTP) vaccine but not by an experimental acellular pertussis vaccine. Since the Bordetella GroEL-like protein was found to contain epitopes similar to those on the mammalian analog, the potential exists that vaccination with standard DTP vaccines may induce antibodies which react with the mammalian GroEL analog.
authors.affiliation: Division of Bacterial Products, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Bethesda, Maryland 20892.
authors.forename: D L
authors.initials: DL
authors.lastname: Burns
authors.forename: J L
authors.initials: JL
authors.lastname: Gould-Kostka
authors.forename: M
authors.initials: M
authors.lastname: Kessel
authors.forename: J L
authors.initials: JL
authors.lastname: Arciniega
mesh_terms: D000818:Animals; D001172:Arthritis, Rheumatoid / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology / Q000302:isolation & purification*; D001886:Bordetella pertussis / Q000032:analysis*; D015721:Diphtheria-Tetanus-Pertussis Vaccine / Q000276:immunology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D014611:Vaccination
chemical_list: D001426:Bacterial Proteins; D015721:Diphtheria-Tetanus-Pertussis Vaccine