19-Hydroxylated steroids, new metabolites produced by the Y1 adrenal cell line.
The expression of 19-hydroxylase activity in the Y1 adrenal cell line is reported here for the first time. Two new metabolites from the incubation of deoxycorticosterone (DOC) with these cells, 19-hydroxy-20 alpha-dihydroDOC and 19-hydroxy-20 alpha-dihydrocorticosterone, have been identified. The most important of the two is the 11 beta,19-dihydroxylated metabolite, which is produced in smaller amounts than 18-hydroxy-20 alpha-dihydrocorticosterone. A third 19-hydroxylated metabolite was identified as 19-hydroxy-20 alpha-dihydroprogesterone, produced from the cholesterol in the serum supplemented medium. These results show that the cytochrome P-450(11)beta of this cell line expresses 19-hydroxylase activity in addition to 11 beta- and 18-hydroxylase activities, as do those of other species.
Es-souni
M
M
Laboratoire de Biochimie des Interactions Cellulaires, Faculté de Sciences, Université de Bourgogne, Dijon, France.
Bournot
P
P
Berlot
J P
JP
Ramirez
L C
LC
D000311:Adrenal Glands / Q000201:enzymology*; D000818:Animals; D002849:Chromatography, Gas; D003900:Desoxycorticosterone / Q000031:analogs & derivatives / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D051379:Mice; D013250:Steroid Hydroxylases / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D003900:Desoxycorticosterone; D013250:Steroid Hydroxylases; C027110:steroid 19-hydroxylase