title: Basal cells of H-Dunning tumor are myoepithelial cells. A comparative immunohistochemical and ultrastructural study with male accessory sex glands and mammary gland.
abstract: Recent immunohistochemical studies have shown that basal cells in human prostatic epithelium are not myoepithelial cells. Since in the literature the Dunning tumor, originally described as a rat prostate carcinoma derived from the dorsolateral prostate of a Copenhagen rat, was reported to have myoepithelial cells, a comparative immunohistochemical and ultrastructural study was performed in the H-, HIF- and AT3-lines of the Dunning tumor, the male accessory sex glands (ventral, dorsal, lateral prostate, coagulating gland, bulbourethral gland) and the mammary gland of both Copenhagen and Wistar rats. Mono- and polyclonal antibodies directed against intermediate filament proteins (cytokeratin, desmin, vimentin) and the contractile proteins (alpha-actin, muscle type specific myosin, tropomyosin) were used along with phalloidin decoration of F-actin. As in the human prostate, none of the rat prostate lobes in either strains did contain basal cells expressing cytokeratin along with alpha-actin, myosin and tropomyosin Cells representing fully differentiated myoepithelial cells, however, were present as anticipated in the mammary gland, the bulbourethral gland and the H-tumor line of the Dunning tumor. This finding is difficult to reconcile with the contention of a prostatic origin of the H-Dunning tumor. Further studies are required to classify the epithelial parental tissue in order to define the true origin of the H-Dunning tumor and the tumor lines derived thereof.
authors.affiliation: Department of Anatomy and Cell Biology, Philipps-Universität, Marburg, Federal Republic of Germany.
authors.forename: G
authors.initials: G
authors.lastname: Aumüller
authors.forename: U
authors.initials: U
authors.lastname: Gröschel-Stewart
authors.forename: M
authors.initials: M
authors.lastname: Altmannsberger
authors.forename: H G
authors.initials: HG
authors.lastname: Mannherz
authors.forename: M
authors.initials: M
authors.lastname: Steinhoff
mesh_terms: D008841:Actin Cytoskeleton / Q000737:chemistry* / Q000648:ultrastructure; D000818:Animals; D015415:Biomarkers; D002030:Bulbourethral Glands / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure; D003285:Contractile Proteins / Q000032:analysis*; D004847:Epithelial Cells; D004848:Epithelium / Q000737:chemistry / Q000648:ultrastructure; D005260:Female; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis*; D007382:Intermediate Filaments / Q000737:chemistry* / Q000648:ultrastructure; D008297:Male; D008321:Mammary Glands, Animal / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure; D008854:Microscopy, Electron; D011467:Prostate / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000648:ultrastructure*; D051381:Rats; D011919:Rats, Inbred Strains; D012669:Seminal Vesicles / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure; D014407:Tumor Cells, Cultured
chemical_list: D015415:Biomarkers; D003285:Contractile Proteins; D007381:Intermediate Filament Proteins