Expression of S14-mRNA and its translational product in differentiating 3T3-L1 cells.
During adipogenic conversion of 3T3-L1 cells S14-mRNA increased from undetectable levels in preadipocytes to high levels in differentiated adipocytes (adipocyte-like cells). In vitro translation of hybrid-selected S14-mRNA revealed an identical protein in 3T3-L1 adipocytes and in rat liver with regard to migration properties in two-dimensional gel electrophoresis. No translation product was found in 3T3-L1 preadipocytes. In conclusion, protein-S14 is very similar or even identical in rat lipogenic tissues and in 3T3-L1 adipocytes. Therefore, this cell line can be employed to elucidate further physiological aspects of protein-S14.
Loos
U
U
Abteilung Innere Medizin I, Medizinische Klinik und Poliklinik Universität Ulm, F.R.G.
Clement
J
J
Behr
M
M
Fischer
S
S
D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism; D000818:Animals; D015152:Blotting, Northern; D002454:Cell Differentiation; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D008099:Liver / Q000166:cytology / Q000378:metabolism; D051379:Mice; D009687:Nuclear Proteins; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis*; D014157:Transcription Factors
D009687:Nuclear Proteins; D011506:Proteins; D012333:RNA, Messenger; C051065:THRSP protein, human; C501477:Thrsp protein, mouse; C501478:Thrsp protein, rat; D014157:Transcription Factors