title: Dietary plant materials and development of diabetes in the BB rat.
abstract: The present study was designed to examine further the impact of individual plant protein sources found in a diabetogenic, cereal-based, rodent laboratory diet, NIH-07 [open formula, nonpurified rat and mouse diet (positive control)], on the development of diabetes. Diabetes-prone BB rats that were pan-T(OX19+)-lymphopenic were fed a low diabetogenic diet during gestation and lactation. Progeny of these rats were fed a normal or autoclaved NIH-07 diet, or one of eight other diets based on the AIN-76A formulation, with modified protein sources as follows: hydrolyzed casein (HC), soybean meal, HC+ trypsin inhibitor (TI) in water (2 mg/mL, wheat germ, alfalfa seeds, Brewer's yeast, red lentils and a plant protein mixture. Feeding soybean meal increased the incidence of diabetes compared with the negative control, HC diet (47% vs. 12% incidence, P = 0.02). Wheat germ, alfalfa seeds and plant protein mixture resulted in an intermediate incidence of diabetes of 33%; the incidence was lower for Brewer's yeast and lentils (20% and 13%). Autoclaving (121 degrees C, 10 min) the NIH-07 diet or the presence of TI in drinking water had a minimal effect on diabetes frequency, suggesting heat-labile plant toxicants were not directly involved. Thus, certain dietary plant protein sources or associated agents may influence the development of spontaneous diabetes in the BB rat.
authors.affiliation: Nutrition Research Division, Health and Welfare Canada, Ottawa, Ontario.
authors.forename: J
authors.initials: J
authors.lastname: Hoorfar
authors.forename: F W
authors.initials: FW
authors.lastname: Scott
authors.forename: H E
authors.initials: HE
authors.lastname: Cloutier
mesh_terms: D000367:Age Factors; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000209:etiology*; D003922:Diabetes Mellitus, Type 1 / Q000209:etiology*; D004044:Dietary Proteins / Q000378:metabolism*; D002523:Edible Grain / Q000502:physiology*; D005526:Food, Formulated; D015994:Incidence; D008297:Male; D010940:Plant Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013966:Thyroiditis / Q000150:complications / Q000473:pathology
chemical_list: D004044:Dietary Proteins; D010940:Plant Proteins