Structure of a growth-blocking peptide present in parasitized insect hemolymph.
Last instar larvae of the insect armyworm, Pseudaletia separata, parasitized with the parasitoid wasp, Apanteles kariyai, do not initiate metamorphosis and, ultimately, the wasp larvae emerge from the host larvae about 10 days after parasitization (Tanaka, T., Agui, N., and Hiruma, K. (1987) Gen. Comp. Endocrinol. 67, 364-374). It is necessary for the parasitoid wasp to perturb the armyworm's endocrinological processes that control normal metamorphosis from larvae to pupae. This endocrinological perturbation allows the parasitoid to complete its larval growth before emerging from the host larvae. It is obligatory for the parasitoid larvae to emerge while the host is still in a larval stage because the sclerotized pupal cuticle is impenetrable for the parasitoid larvae. A growth-blocking peptide with repressive activity against juvenile hormone esterase has been proven to exist in the parasitized host larval plasma (Hayakawa, Y. (1990) J. Biol. Chem. 265, 10813-10816). Here, I describe the detailed structure of this peptide and also the corresponding synthetic peptide to confirm this structure.
Hayakawa
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Biochemical Laboratory, Hokkaido University, Sapporo, Japan.
D000595:Amino Acid Sequence; D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors*; D002851:Chromatography, High Pressure Liquid; D016207:Cytokines; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D007301:Insect Hormones / Q000302:isolation & purification / Q000378:metabolism*; D019476:Insect Proteins; D007814:Larva; D007915:Lepidoptera / Q000201:enzymology / Q000254:growth & development / Q000469:parasitology*; D008675:Metamorphosis, Biological; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014863:Wasps / Q000472:pathogenicity*
D016207:Cytokines; D004791:Enzyme Inhibitors; D007301:Insect Hormones; D019476:Insect Proteins; D010455:Peptides; C068690:growth blocking peptide, Apanteles kariyai; D002265:Carboxylic Ester Hydrolases; C038003:juvenile hormone esterase