title: Eukaryotic DNA primase. Abortive synthesis of oligoadenylates.
abstract: Calf thymus DNA polymerase alpha-primase, human placenta DNA polymerase alpha-primase and human placenta DNA primase synthesized oligoriboadenylates of a preferred length of 2-10 nucleotides and multimeric oligoribonucleotides of a modal length of about 10 monomers on a poly(dT) template. The dimer and trimer were the prevalent products of the polymerization reaction. However, only the oligonucleotides from heptamers to decamers were elongated efficiently by DNA polymerase alpha.
authors.affiliation: Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk.
authors.forename: V N
authors.initials: VN
authors.lastname: Podust
authors.forename: O V
authors.initials: OV
authors.lastname: Vladimirova
authors.forename: E N
authors.initials: EN
authors.lastname: Manakova
authors.forename: O I
authors.initials: OI
authors.lastname: Lavrik
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D004257:DNA Polymerase II / Q000378:metabolism*; D019915:DNA Primase; D006801:Humans; D009843:Oligoribonucleotides / Q000378:metabolism*; D010920:Placenta / Q000201:enzymology*; D011071:Poly T / Q000378:metabolism; D012316:RNA Nucleotidyltransferases / Q000378:metabolism*; D013698:Templates, Genetic
chemical_list: D000227:Adenine Nucleotides; D009843:Oligoribonucleotides; D011071:Poly T; C023505:2',5'-oligoadenylate; D000255:Adenosine Triphosphate; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases; D004257:DNA Polymerase II