title: Nucleotide sequence of barley yellow mosaic virus RNA 2.
abstract: The sequence of the 3585 nucleotides [excluding the 3' poly(A) tail] of barley yellow mosaic virus (BaYMV) RNA 2 was obtained by analysis of cDNA clones and by direct RNA sequencing. The first initiation codon at nucleotide 155 was followed by a single long open reading frame encoding a protein of 890 amino acids with an Mr of 98,458. Amino acid sequence comparisons indicated that the BaYMV 98K protein contains a region similar to the C-terminal proteinase domain of the potyvirus helper component (HC) protein towards its N terminus, but that it has no sequences that resemble the N-terminal part of the HC protein or other proteins of potyviruses. The data reveal striking differences in genetic organization between BaYMV RNA 2 and the 5'-terminal region of the potyvirus genome, despite a close genetic relationship between BaYMV RNA 1 and the rest of the potyvirus genome.
authors.affiliation: National Agriculture Research Center, Ibaraki, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kashiwazaki
authors.forename: Y
authors.initials: Y
authors.lastname: Minobe
authors.forename: H
authors.initials: H
authors.lastname: Hibino
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D001467:Hordeum / Q000382:microbiology; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics*; D016366:Open Reading Frames; D012367:RNA, Viral / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D003062:Codon; D012367:RNA, Viral