Preliminary X-ray analysis of crystals of plasminogen activator inhibitor-1.
Crystals of bacterially expressed plasminogen activator inhibitor (PAI-1) suitable for X-ray diffraction analysis have been obtained from 8% (w/v) PEG 1500, pH 8.25. The space group is P1, and the lattice constants are a = 82.17 A, b = 47.82 A, c = 62.89 A, alpha = 90.00 degrees, beta = 106.90 degrees, gamma = 106.84 degrees. The diffraction limit is 2.3 A, and the unit cell contains two molecules of PAI-1. The crystals contain latent PAI-1 which can be partly reactivated by exposure to denaturants.
Goldsmith
E J
EJ
Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235-9050.
Sheng-Cheng
C
C
Danley
D E
DE
Gerard
R D
RD
Geoghegan
K F
KF
Mottonen
J
J
Strand
A
A
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003460:Crystallization; D008715:Methionine / Q000737:chemistry; D008969:Molecular Sequence Data; D015849:Plasminogen Inactivators / Q000737:chemistry*; D014961:X-Ray Diffraction
D015849:Plasminogen Inactivators; D008715:Methionine