title: Microassay for DNA methyltransferase.
abstract: A microassay for DNA methylase is described which can detect activity in as few as 50 tissue culture cells. The cells are lysed and incubated for 2 h at 37 degrees C with 3 microCi high specific activity [3H]AdoMet and 0.5 microgram poly[d(I-C).d(I-C)] in a volume of 23 microliters. Ribonuclease is present during the assay and the product DNA is isolated by phenol extraction after protease digestion.
authors.affiliation: Department of Biochemistry, University of Glasgow, UK.
authors.forename: R L
authors.initials: RL
authors.lastname: Adams
authors.forename: A
authors.initials: A
authors.lastname: Rinaldi
authors.forename: C
authors.initials: C
authors.lastname: Seivwright
mesh_terms: D015254:DNA Modification Methylases / Q000032:analysis*; D007700:Kinetics; D008832:Microchemistry / Q000379:methods; D013379:Substrate Specificity
chemical_list: D015254:DNA Modification Methylases