Killing of pathogens associated with chronic granulomatous disease by the non-oxidative microbicidal mechanisms of human neutrophils.
The susceptibility of opportunist pathogens associated with chronic granulomatous disease (CGD) to the non-oxidative killing mechanisms of neutrophils has been assessed by incubation in human neutrophil primary granule lysate. The dose and pH-dependency of killing of Aspergillus fumigatus, Candida albicans, Escherichia coli, Nocardia asteroides, Serratia marcescens and Staphylococcus aureus differed markedly and may partly explain their virulence in CGD, in which oxygen-dependent killing mechanisms are defective. At the acid pH in CGD neutrophil phagosomes S. aureus, Ser. marcescens, N. asteroides and A. fumigatus spores were highly resistant but C. albicans, a less frequent pathogen in patients with CGD, was much more susceptible.
Odell
E W
EW
Department of Oral Medicine and Pathology, UMDS, Guy's Hospital, London.
Segal
A W
AW
D001232:Aspergillus fumigatus / Q000472:pathogenicity; D001419:Bacteria / Q000472:pathogenicity; D001770:Blood Bactericidal Activity; D002176:Candida albicans / Q000472:pathogenicity; D006105:Granulomatous Disease, Chronic / Q000276:immunology* / Q000382:microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008954:Models, Biological; D009504:Neutrophils / Q000276:immunology*; D014774:Virulence