Interferences in current methods for measurements of creatinine.
We measured the interference of carbonyl compounds, drugs, and other substances in human serum on the determination of creatinine by the two-point, fixed-time kinetic modification of the Jaffé reaction as well as by four enzymatic methods. We added known concentrations of the interfering substances to a solution of creatinine in water. For bilirubin, we used both pooled normal sera with added bilirubin and icteric patient sera. The magnitude of interferences varies widely from method to method. Carbonyl compounds, dopamine, cephalosporines, and bilirubin interfere with the Jaffé reaction. Bilirubin, creatine, dopamine, ascorbic acid, and sarcosine interfere with the enzymatic methods. We conclude that the elimination of interferences in the determination of creatinine has still not been achieved.
Weber
J A
JA
Department of Clinical Chemistry, Slotervaart Hospital, Amsterdam, The Netherlands.
van Zanten
A P
AP
D001774:Blood Chemical Analysis; D003404:Creatinine / Q000097:blood*; D005189:False Positive Reactions; D006801:Humans; D008722:Methods; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D003404:Creatinine