Application of a simple extraction procedure using aqueous ammonia to the analysis of basic drugs in blood by gas chromatography.
A simple and reliable previously reported extraction procedure has been extended to the analysis of a wide range of basic and neutral drugs in postmortem blood and other tissues. The method employs 200 microliters of blood treated with water and ammonia and extracted with diethyl ether. The concentrated extract is generally sufficiently clean for direct analysis by gas chromatography and high pressure liquid chromatography. Recovery, precision and linearity data are presented for selected drugs.
Sims
D N
DN
State Forensic Science, Forensic Science Centre, Adelaide, South Australia.
Felgate
P D
PD
Felgate
H E
HE
Lokan
R J
RJ
D000641:Ammonia; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D004308:Dothiepin / Q000097:blood; D006801:Humans; D004364:Pharmaceutical Preparations / Q000032:analysis*; D015203:Reproducibility of Results
D004364:Pharmaceutical Preparations; D000641:Ammonia; D004308:Dothiepin