Characterization of the sugarcane streak agent as a distinct geminivirus.
The relationship between maize streak virus and the geminivirus causing streak in sugarcane was investigated. The DNA of sugarcane streak virus does not cross-hybridize detectably with that of maize streak virus and vice versa. Restriction mapping of native replicative form viral DNA (genome size 2.7 kb) and of cloned viral DNA, combined with limited sequencing and estimated DNA sequence divergence, showed that sugarcane streak virus is as unrelated to maize streak virus and digitaria streak virus as these are different from each other. The virus is only distantly related to wheat dwarf virus and chloris striate mosaic virus. Based on these results, we propose that the agent causing sugarcane streak is a distinct geminivirus.
Hughes
F L
FL
Department of Microbiology, University of Cape Town: Republic of South Africa.
Rybicki
E P
EP
Kirby
R
R
von Wechmar
M B
MB
D015153:Blotting, Western; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D014644:Genetic Variation; D016253:Microscopy, Immunoelectron; D010802:Phylogeny; D010942:Plant Viruses / Q000145:classification* / Q000235:genetics; D015183:Restriction Mapping; D003313:Zea mays / Q000382:microbiology
D004279:DNA, Viral