title: Preliminary X-ray analysis of crystals of plasminogen activator inhibitor-1.
abstract: Crystals of bacterially expressed plasminogen activator inhibitor (PAI-1) suitable for X-ray diffraction analysis have been obtained from 8% (w/v) PEG 1500, pH 8.25. The space group is P1, and the lattice constants are a = 82.17 A, b = 47.82 A, c = 62.89 A, alpha = 90.00 degrees, beta = 106.90 degrees, gamma = 106.84 degrees. The diffraction limit is 2.3 A, and the unit cell contains two molecules of PAI-1. The crystals contain latent PAI-1 which can be partly reactivated by exposure to denaturants.
authors.affiliation: Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235-9050.
authors.forename: E J
authors.initials: EJ
authors.lastname: Goldsmith
authors.forename: C
authors.initials: C
authors.lastname: Sheng-Cheng
authors.forename: D E
authors.initials: DE
authors.lastname: Danley
authors.forename: R D
authors.initials: RD
authors.lastname: Gerard
authors.forename: K F
authors.initials: KF
authors.lastname: Geoghegan
authors.forename: J
authors.initials: J
authors.lastname: Mottonen
authors.forename: A
authors.initials: A
authors.lastname: Strand
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003460:Crystallization; D008715:Methionine / Q000737:chemistry; D008969:Molecular Sequence Data; D015849:Plasminogen Inactivators / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D015849:Plasminogen Inactivators; D008715:Methionine