Interaction of mitochondrial creatine kinase with model membranes. A monolayer study.
The interaction of mitochondrial creatine kinase (Mi-CK; EC 2.7.3.2) with phospholipid monolayers and spread mitochondrial membranes at the air/water interface has been investigated. It appeared that Mi-CK penetrated into these monolayers as evidenced by an increase in surface pressure upon incorporation of Mi-CK. The increase in surface pressure was dependent on (1) the amount and (2) the oligomeric form of Mi-CK in the subphase, as well as on (3) the initial surface pressure and (4) the phospholipid composition of the monolayer. In this experimental system Mi-CK was able to interact equally well with both inner and outer mitochondrial membranes.
Rojo
M
M
Institute for Cell Biology, Eidgenössische Technische Hochschule, Zürich, Switzerland.
Hovius
R
R
Demel
R
R
Wallimann
T
T
Eppenberger
H M
HM
Nicolay
K
K
D000818:Animals; D002645:Chickens; D003402:Creatine Kinase / Q000302:isolation & purification / Q000378:metabolism*; D007527:Isoenzymes; D007700:Kinetics; D008081:Liposomes; D008929:Mitochondria, Heart / Q000201:enzymology*; D008931:Mitochondria, Muscle / Q000201:enzymology*; D010743:Phospholipids / Q000378:metabolism; D011312:Pressure; D013499:Surface Properties
D007527:Isoenzymes; D008081:Liposomes; D010743:Phospholipids; D003402:Creatine Kinase