Cell labelling with 67Ga-citrate.
A method of cell labelling with carrier-free 67Ga-citrate, based on a co-binding with iron from ferric lactate has been developed. 67Ga-binding has a direct relationship with the iron incorporation by the cell. Using this method Ehrlich ascites tumor cells increase their uptake from 5% (no ferric lactate) to 89% (5 mumol of ferric lactate). Erythrocytes have shown the same pattern of 67Ga-binding. In vivo cytotoxic assays (ascites formation and animal survival) have demonstrated the lack of cytotoxicity of ferric lactate under these experimental conditions. Electrophoretic analyses of 67Ga-labelled tumor cells and erythrocytes have shown the absence of free 67Ga-citrate.
Anghileri
L J
LJ
Nuclear Medicine Service, Faculty of Medicine, University of Nancy, France.
D000328:Adult; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000000981:diagnostic imaging / Q000378:metabolism; D002951:Citrates / Q000493:pharmacokinetics*; D019343:Citric Acid; D004912:Erythrocytes / Q000000981:diagnostic imaging / Q000378:metabolism; D005710:Gallium Radioisotopes / Q000493:pharmacokinetics*; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D007773:Lactates; D019344:Lactic Acid; D051379:Mice; D011877:Radionuclide Imaging; D014407:Tumor Cells, Cultured / Q000000981:diagnostic imaging / Q000378:metabolism
D002951:Citrates; D005710:Gallium Radioisotopes; D007773:Lactates; D019343:Citric Acid; D019344:Lactic Acid