Two pathways in the biosynthesis of cadystins (gamma EC)nG in the cell-free system of the fission yeast.
Small metal-binding peptides, cadystins, with the general structure of (gamma-Glu-Cys)n-Gly ((gamma EC)nG), were synthesized in a cell-free system of fission yeast to examine the in vivo synthetic pathway. The crude enzyme for cadystin synthesis was prepared by ammonium sulfate precipitation (75% saturation) from the 120,000 x g supernatant of the cell extract, and the excess salt in the enzyme fraction was removed by Sephadex gel filtration. Using this crude enzyme fraction, it was shown that there were two pathways for cadystin biosynthesis. One pathway is gamma-Glu-Cys (gamma EC) dipeptidyl transfer from both glutathione (gamma ECG) and cadystins to glutathione and cadystins. The other one is gamma EC polymerization from (gamma EC)n and glutathione to (gamma EC)n + i, followed by glycine addition with glutathione synthetase.
Hayashi
Y
Y
Institute for Developmental Research, Aichi, Japan.
Nakagawa
C W
CW
Mutoh
N
N
Isobe
M
M
Goto
T
T
D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000378:metabolism; D020789:Cathepsin C; D002474:Cell-Free System; D002851:Chromatography, High Pressure Liquid; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000378:metabolism; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005981:Glutathione Synthase / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins; D007700:Kinetics; D008969:Molecular Sequence Data; D012568:Schizosaccharomyces / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins; D013056:Spectrophotometry, Ultraviolet
D002352:Carrier Proteins; D005656:Fungal Proteins; D047908:Intracellular Signaling Peptides and Proteins; D029702:Schizosaccharomyces pombe Proteins; C055088:cadystin protein, S pombe; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D020789:Cathepsin C; D005981:Glutathione Synthase; D005978:Glutathione; D005998:Glycine