Self-assembled organic monolayers: model systems for studying adsorption of proteins at surfaces.
Self-assembled monolayers (SAMs) of omega-functionalized long-chain alkanethiolates on gold films are excellent model systems with which to study the interactions of proteins with organic surfaces. Monolayers containing mixtures of hydrophobic (methyl-terminated) and hydrophilic [hydroxyl-, maltose-, and hexa(ethylene glycol)-terminated] alkanethiols can be tailored to select specific degrees of adsorption: the amount of protein adsorbed varies monotonically with the composition of the monolayer. The hexa(ethylene glycol)-terminated SAMs are the most effective in resisting protein adsorption. The ability to create interfaces with similar structures and well-defined compositions should make it possible to test hypotheses concerning protein adsorption.
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KL
Department of Chemistry, Harvard University, Cambridge, MA 02138.
Whitesides
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GM
D000327:Adsorption; D000473:Alkanes; D005340:Fibrinogen / Q000737:chemistry; D007700:Kinetics; D008956:Models, Chemical; D011506:Proteins / Q000737:chemistry*; D011770:Pyruvate Kinase / Q000737:chemistry; D012260:Ribonucleases / Q000737:chemistry; D013499:Surface Properties
D000473:Alkanes; D011506:Proteins; D005340:Fibrinogen; D011770:Pyruvate Kinase; D012260:Ribonucleases