Changes in glial fibrillary acidic protein mRNA expression after corticospinal axotomy in the adult hamster.
We examined changes in the expression of glial fibrillary acidic protein (GFAP) mRNA during Wallerian degeneration in the corticospinal system of the adult Golden hamster following axotomy. GFAP is the product of a type III intermediate filament (IF) gene that is expressed specifically in mature astrocytes. A well-studied component of a complex response termed reactive astrogliosis that occurs after various types of CNS injury is the increased production of astrocytic processes filled with GFAP-containing IFs. While increased expression of GFAP during reactive astrogliosis has been well established at the protein level, little is known about whether or not changes in GFAP mRNA levels occur after CNS injury. In the present study we used in situ hybridization methods to examine this issue. A 35S-labeled mouse GFAP cDNA probe was used for in situ hybridizations of sections of the brain stem obtained 2, 7, and 14 days after unilateral transections of the corticospinal tract in the caudal medulla. Film as well as emulsion autoradiography showed a dramatic increase in GFAP mRNA labeling associated with the degenerating corticospinal tract. GFAP mRNA levels were already dramatically increased in the injured corticospinal tract by 2 days post axotomy and remained elevated at 14 days. Interestingly, in addition to the robust increase in GFAP mRNA levels specifically associated with the degenerating tract, a diffuse increase in GFAP mRNA labeling was observed throughout the grey matter of the brain stem at 2 days post-axotomy, but not after this time. Immunoblotting and immunocytochemical experiments verified that the increased GFAP mRNA levels in the degenerating corticospinal system were accompanied by an increased expression of the protein. These results demonstrate that an increase in GFAP mRNA levels occurs during Wallerian degeneration in the CNS and suggest that increased expression of the GFAP gene is a major contributor to CNS scarring that results after direct traumatic injury.
Kost-Mikucki
S A
SA
Department of Cell Biology and Anatomy, Chicago Medical School, IL 60064.
Oblinger
M M
MM
D000818:Animals; D001345:Autoradiography; D001369:Axons / Q000502:physiology*; D002540:Cerebral Cortex / Q000502:physiology* / Q000648:ultrastructure; D006224:Cricetinae; D003714:Denervation; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics*; D007150:Immunohistochemistry; D008297:Male; D008647:Mesocricetus; D012333:RNA, Messenger / Q000378:metabolism*; D013116:Spinal Cord / Q000502:physiology* / Q000648:ultrastructure
D005904:Glial Fibrillary Acidic Protein; D012333:RNA, Messenger