The in vitro metabolic activation of dibenz[a,h]anthracene, catalyzed by by rat liver microsomes and examined by 32P-postlabelling.
DNA has been incubated in vitro with dibenz[a,h]anthracene (DB[a,H]A) and the related 5,6-diol and 3,4-diol in the presence of 3-methylcholanthrene- or Aroclor 1254-induced rat liver microsomes. After incubation, the DNA was extracted and examined for the presence of aromatic adducts using the nuclease P1 modification of the 32P-postlabelling technique. The maps of PEI-cellulose plates and autoradiography showed that 92% of the radioactivity contained in DB[a,h]A-DNA adduct spots is derived from the related 3,4-diol and that about 50% of the adducts may be formed following the conversion of this diol to the bay-region anti- and syn-3,4-diol 1,2-oxides.
Lecoq
S
S
Haddow Laboratories, Institute of Cancer Research, Sutton, Surrey, U.K.
Ni Shé
M
M
Grover
P L
PL
Platt
K L
KL
Oesch
F
F
Phillips
D H
DH
D000818:Animals; D001140:Aroclors / Q000494:pharmacology; D001551:Benz(a)Anthracenes / Q000378:metabolism*; D001711:Biotransformation; D004249:DNA Damage; D004852:Epoxy Compounds / Q000737:chemistry; D066298:In Vitro Techniques; D008748:Methylcholanthrene / Q000494:pharmacology; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats
D001140:Aroclors; D001551:Benz(a)Anthracenes; D004852:Epoxy Compounds; D008748:Methylcholanthrene; C026486:1,2,5,6-dibenzanthracene