title: Cultured vascular endothelial cells secrete a protein factor(s) that promotes the contraction of collagen lattices made with fibroblasts.
abstract: Conditioned media collected from arterial endothelial cells contain protein factor(s) that promotes the contraction of collagen lattices made with skin fibroblasts. Based on the lattice contraction-promoting activity, a protein with an apparent molecular weight of 22 kDa was identified. This 22-kDa protein stimulated lattice contraction in both serum-containing and serum-free media. When assayed at a 30% equivalent of the conditioned medium, the contraction-promoting activity of the purified factor was about 50 to 60% of that elicited by the unfractionated conditioned medium. Some contraction-promoting activity was also present in certain subfractions of the conditioned medium generated during the separation of the 22-kDa protein. Taken together, the results indicate that the lattice contraction-promoting activity in the endothelial cell-conditioned medium is probably aided by multiple active principles. The biochemical and biological characteristics indicated that the 22-kDa protein is not a transforming growth factor-beta-related factor nor a fibroblast growth promoter.
authors.affiliation: Department of Physiology, Chang Gung Medical College, Taiwan, Republic of China.
authors.forename: J K
authors.initials: JK
authors.lastname: Chen
authors.forename: G
authors.initials: G
authors.lastname: Green
authors.forename: C B
authors.initials: CB
authors.lastname: Weinberg
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003094:Collagen / Q000378:metabolism*; D003470:Culture Media; D004730:Endothelium, Vascular / Q000378:metabolism*; D005109:Extracellular Matrix / Q000502:physiology*; D005347:Fibroblasts / Q000378:metabolism; D005782:Gels; D006801:Humans; D066298:In Vitro Techniques; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000502:physiology; D011506:Proteins / Q000737:chemistry / Q000502:physiology; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
chemical_list: D003470:Culture Media; D005782:Gels; D011506:Proteins; D016212:Transforming Growth Factor beta; D003094:Collagen; D014357:Trypsin