title: Transport of cyclosporin A in kidney epithelial cell line (LLC-PK1).
abstract: The characteristics of cyclosporin A transport have been studied in cultured kidney epithelial cell line LLC-PK1. The uptake of cyclosporin A by LLC-PK1 cells was time-dependent and reached a steady state at about 30 min. The initial uptake was saturable and was inhibited by cyclosporin A analogs, cyclosporin C and D and by verapamil, but not by metabolic inhibitors such as 2,4-dinitrophenol and rotenone. Cyclosporin A uptake as well as efflux was strongly dependent on temperature. The Arrhenius plot for cyclosporin A uptake was biphasic, whereas the Arrhenius plot for sulfanilamide uptake, which is transported by a simple diffusion, was linear. These results indicate that a specific mechanism is concerned in the transport of cyclosporin A in LLC-PK1, cells.
authors.affiliation: Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Kyoto University, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Takayama
authors.forename: Y
authors.initials: Y
authors.lastname: Okazaki
authors.forename: K
authors.initials: K
authors.lastname: Fukuda
authors.forename: M
authors.initials: M
authors.lastname: Takano
authors.forename: K
authors.initials: K
authors.lastname: Inui
authors.forename: R
authors.initials: R
authors.lastname: Hori
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002460:Cell Line; D003524:Cyclosporins / Q000493:pharmacokinetics* / Q000633:toxicity; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D013552:Swine; D013696:Temperature; D014700:Verapamil / Q000494:pharmacology
chemical_list: D003524:Cyclosporins; D014700:Verapamil