title: Kinetic differences in the effect of calcium on quantal and non-quantal acetylcholine release at the murine diaphragm.
abstract: The effects of Ca2+ withdrawal on non-quantal, evoked quantal and spontaneous quantal release of acetylcholine (ACh) from the motor nerve terminals were studied with standard intracellular recording techniques. Anticholinesterase-treated mouse diaphragms were used. In a Ca2(+)-free solution all forms of ACh release decreased, but with different kinetics. As expected, evoked quantal release declined to zero within a few minutes. Spontaneous quantal release, i.e. the frequency of the miniature end-plate potentials (MEPPs), decreased to 15% of the control within 20 min after calcium withdrawal. The slowest decay was that of non-quantal release which declined very slowly and reached zero after 45-50 min. Following Ca2+ re-admission, both quantal types of ACh release were rapidly restored (evoked in 10 min, spontaneous in 20 min). However, recovery of non-quantal release did not occur until after 50 to 60 min.
authors.affiliation: Kazan Medical Institute, Tatar Autonomic Republik, U.S.S.R.
authors.forename: E E
authors.initials: EE
authors.lastname: Nikolsky
authors.forename: V A
authors.initials: VA
authors.lastname: Voronin
authors.forename: F
authors.initials: F
authors.lastname: Vyskocil
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D003964:Diaphragm / Q000378:metabolism / Q000502:physiology; D004594:Electrophysiology; D005260:Female; D007700:Kinetics; D051379:Mice; D009045:Motor Endplate / Q000502:physiology; D009132:Muscles / Q000378:metabolism* / Q000502:physiology; D011930:Reaction Time; D014403:Tubocurarine / Q000494:pharmacology
chemical_list: D002800:Cholinesterase Inhibitors; D000109:Acetylcholine; D002118:Calcium; D014403:Tubocurarine