Structure of the yeast ribosomal 5 S RNA-binding protein YL3.
A gene coding for the 5 S rRNA-binding protein (YL3) in yeast (Saccharomyces cerevisiae) was isolated using a polymerase chain reaction-amplified gene probe. The DNA sequence contains no introns and codes for a 297 amino acid (Mr = 33,741) protein. Although the protein is just 1 residue longer than in rat, unlike the high sequence homology in the 5 S rRNAs from the same organisms, only about 45% of the amino acid residues are conserved with surprisingly little homology in the carboxyl-terminal end. Nevertheless, comparative studies indicate that a number of structural features are conserved including small repeats in the primary structure and a number of helical estimates in the higher order structure. One of the sequence repeats also appears to be present in the carboxyl-terminal end of the eukaryotic transcription factor TFIIIA suggesting an evolutionary relationship in these 5 S RNA-binding proteins.
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Department of Molecular Biology and Genetics, University of Guelph, Ontario, Canada.
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D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D016601:RNA-Binding Proteins; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D012269:Ribosomal Proteins; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D012689:Sequence Homology, Nucleic Acid
D002352:Carrier Proteins; D004271:DNA, Fungal; D012341:RNA, Ribosomal, 5S; D016601:RNA-Binding Proteins; C489268:RPL5 protein, S cerevisiae; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins