NGF enhances neurite regeneration from nerve-transected terminals of young adult and aged mouse dorsal root ganglia in vitro.
Dorsal root ganglia with nerve fibers, dissected from 3-month-old young adult mice and 26-month-old aged mice, were embedded in collagen gel. In the young adult mice regenerating neurites appeared from nerve-transected terminals after 12-24 h in culture, regardless of the length of fibers, but in the aged mice, regenerating neurites were first seen after 12-48 h. Administration of nerve growth factor to the explants markedly enhanced the growth of regenerating neurites from both the young adult and aged mice.
Horie
H
H
Department of Physiology, School of Medicine, Yokohama City University, Japan.
Bando
Y
Y
Chi
H
H
Takenaka
T
T
D000375:Aging / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D003094:Collagen; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D009411:Nerve Endings / Q000187:drug effects*; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009416:Nerve Regeneration / Q000187:drug effects*
D009414:Nerve Growth Factors; D003094:Collagen