Enhanced antiproliferative activity by metastatic RAW117 lymphoma cells.
The highly malignant/metastatic murine large cell lymphoma cell line RAW117-H10 forms 100-200 times more liver metastatic tumors than its parental counterpart cell line RAW117-P. RAW117-H10 cells, but not the less malignant/metastatic parental cells, significantly inhibited the mitogen-induced proliferation of normal syngeneic Balb/c and allogeneic ICRC mouse spleen cells. Such an inhibition also occurred when mitomycin-C treated metastatic lymphoma cells were added 24 h after initiation of culture, indicating that no competition with mitogen binding sites on the lymphocytes was necessary for inhibition of proliferation. 'Antiproliferative' cell surface molecules were extracted non-cytolytically from the RAW117-H10 cells using butanol. The butanol extracts from the metastatic RAW117-H10 cells also inhibited the mitogen-induced proliferation and natural killer (NK) cell-mediated cytotoxicity of normal spleen cells. Our results indicate that these 'antiproliferative' cell surface molecules of metastatic murine RAW117-H10 lymphoma cells may have important role(s) in tumor-mediated host immunosuppression.
Joshi
S S
SS
Department of Anatomy, University of Nebraska Medical Center Omaha 68198-6395.
O'Connor
S J
SJ
Weisenburger
D D
DD
Sharp
J G
JG
Gharpure
H M
HM
Brunson
K W
KW
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003603:Cytotoxins / Q000494:pharmacology*; D007108:Immune Tolerance; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000187:drug effects; D008223:Lymphoma / Q000737:chemistry* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009362:Neoplasm Metastasis; D013154:Spleen / Q000473:pathology*; D014407:Tumor Cells, Cultured
D003603:Cytotoxins; D007166:Immunosuppressive Agents