Structural analysis of biologically active peptides and recombinant proteins and their modified counterparts by mass spectrometry.
The structural characterization of the Escherichia coli-expressed human interferon alpha-2b (rh-IFN alpha-2b) was carried out by employing the fast atom bombardment (FAB) and plasma desorption (PD) mapping methods. The mass spectral data of the rh-IFN alpha-2b and the trypsin-generated peptide mixture allowed rapid and facile confirmation of the cDNA-derived sequence and determination of the existing disulfide pattern in the protein molecule. The same PD/FAB mapping approach was successfully employed in the structural determination of the iodination reaction product of rh-IFN alpha-2b and the potent vasoconstrictor peptide endothelin.
Pramanik
B N
BN
Schering-Plough Research, Bloomfield, NJ 07003.
Tsarbopoulos
A
A
Labdon
J E
JE
Trotta
P P
PP
Nagabhushan
T L
TL
D000595:Amino Acid Sequence; D001483:Base Sequence; D003545:Cysteine / Q000737:chemistry; D016232:Endothelins / Q000032:analysis / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000032:analysis; D006801:Humans; D006868:Hydrolysis; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000032:analysis; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis*; D011994:Recombinant Proteins / Q000032:analysis*; D012697:Serine Endopeptidases; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014357:Trypsin
D016232:Endothelins; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D010455:Peptides; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D014357:Trypsin; D003545:Cysteine