Evaluation of 31P NMR spectroscopy as an indicator of chemically induced hepatic toxicity in the rat: comparison with serum enzyme levels and pathology.
The progression of carbon tetrachloride-induced liver damage, determined by 31P NMR spectroscopy, was compared with selected serum enzyme and histological changes in rats. ATP levels declined as early as 8 h post-CCl4 administration, with partial recovery observed at 168 h. The results show that ATP reduction correlates with necrosis. In addition, early decline in ATP occurring prior to significant hepatocellular necrosis indicates abnormal energy metabolism.
Sandhu
G S
GS
Research Department, CIBA-GEIGY Corporation, Summit, NJ 07901.
Gonnella
N C
NC
Kapeghian
J C
JC
Plocinski
A F
AF
Plutchok
J
J
Schlosser
M J
MJ
D000255:Adenosine Triphosphate / Q000097:blood*; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001123:Argininosuccinate Lyase / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D002251:Carbon Tetrachloride / Q000633:toxicity; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D000255:Adenosine Triphosphate; D002251:Carbon Tetrachloride; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D001123:Argininosuccinate Lyase