Interstrand cross-linking of the hexadeoxynucleotide d(TACGTA) upon reaction with trans-diamminedichloroplatinum(II).
The reaction of trans-diamminedichloroplatinum(II), an inactive isomer of cisplatin, with d(TACGTA) was performed in aqueous solution, and the interstrand cross-linked adduct was isolated on a preparative scale. This adduct was characterized by 1 M thiourea treatment, atomic absorption spectroscopy, enzymatic digestion and 1H NMR as platinum coordinated to two molecules of d(TACGTA) at the N7 atom of the guanine residue. This interstrand cross-linked adduct did not form a duplex structure in the conditions where the free hexanucleotide adopts it.
Urata
H
H
Osaka University of Pharmaceutical Sciences, Japan.
Ueno
M
M
Yamasaki
F
F
Akagi
M
M
D002851:Chromatography, High Pressure Liquid; D002945:Cisplatin / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004249:DNA Damage; D006147:Guanine / Q000737:chemistry; D006860:Hydrogen Bonding; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013237:Stereoisomerism
D003432:Cross-Linking Reagents; D009838:Oligodeoxyribonucleotides; C034697:transplatin; D006147:Guanine; D002945:Cisplatin