Enzyme immunoassay for plasma estradiol using a monoclonal antibody.
A microtitre plate enzyme immunoassay (EIA) for plasma estradiol is described, involving competition between sample estradiol and an immobilized estradiol-bovine serum albumin complex for a monoclonal anti-estradiol antibody, followed by immobilized antibody quantitation using enzyme-labelled antiglobulins. The assay dose-response curve covered a range of 6-1500 fmol/well. The intra- and inter-assay coefficient of variation for the assay of three plasma pools ranged from 3.1 to 4.7% and from 4.7 to 10.6% respectively. The assay showed satisfactory correlation with a standard estradiol radioimmunoassay. Pre-coated microtitre plates were stable, dried, at 4 degrees C for up to 3 months and the anti-estradiol was stable to lyophilization and also was stable in solution at 4 degrees C for up to 1 month.
el Jabri
J
J
UFR Biomédicale des Saints-Pères, Département de Biochimie, Paris, France.
D000911:Antibodies, Monoclonal; D003429:Cross Reactions; D004958:Estradiol / Q000097:blood*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D004958:Estradiol