Destruction of tumour parenchyma in basal cell carcinoma by tumour-associated neutral proteases: a histochemical study.
Proteolytic activity was demonstrated histochemically in frozen sections of basal cell carcinomas (BCCs). After incubation of tissue sections in 0.1 M phosphate buffer with 0.25 M NaCl the tumour epithelium was almost completely destroyed. The basal and squamous cell layers of the epidermis disintegrated to varying degrees, particularly where they were directly in contact with tumour epithelium. Serine and metalloprotease inhibitors diminished this tissue destruction. Iodoacetate enhanced tumour destruction, urea and potassium thiocyanate even more so. The high proteolytic activity of BCC demonstrated in this study may be an important factor in the proliferative, invasive and destructive behaviour of this tumour.
Schlagenhauff
B
B
Department of Anatomy, University of Tübingen, Germany.
Klessen
C
C
Teichmann-Dörr
S
S
Breuninger
H
H
Rassner
G
G
D002280:Basal Cell Carcinoma / Q000201:enzymology* / Q000473:pathology; D004396:Coloring Agents / Q000494:pharmacology; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D010450:Endopeptidases / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D007461:Iodoacetates / Q000494:pharmacology; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors; D009361:Neoplasm Invasiveness; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D012867:Skin / Q000201:enzymology; D012878:Skin Neoplasms / Q000201:enzymology* / Q000473:pathology; D012965:Sodium Chloride / Q000494:pharmacology; D013861:Thiocyanates / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology
D004396:Coloring Agents; D015853:Cysteine Proteinase Inhibitors; D007461:Iodoacetates; D015842:Serine Proteinase Inhibitors; D013861:Thiocyanates; D012965:Sodium Chloride; D014508:Urea; D010450:Endopeptidases; D008666:Metalloendopeptidases; C009941:potassium thiocyanate