Receptor classification using antagonists without determining pA2 values.
Albeit time-consuming, the most rigorous method of classification of receptor subtypes is the determination of pA2 values. However, this methodology requires the knowledge of absolute concentrations of both agonists and antagonists--something that may not necessarily be calculable under certain experimental conditions. Wolfgang Kromer offers a different method for classification using antagonists which is not dependent on absolute affinities, and which has the additional advantage of speed.
Kromer
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Department of Pharmacology, Byk Gulden Pharmaceuticals, Konstanz, FRG.
D000470:Alkaloids / Q000494:pharmacology*; D001667:Binding, Competitive; D005663:Furans; D018727:Muscarinic Antagonists; D009281:Naphthalenes; D010880:Piperidines; D010890:Pirenzepine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D011976:Receptors, Muscarinic / Q000145:classification*
D000470:Alkaloids; D005663:Furans; D018727:Muscarinic Antagonists; D009281:Naphthalenes; D010880:Piperidines; D011976:Receptors, Muscarinic; C046338:telenzepine; D010890:Pirenzepine; C048172:himbacine