title: Cytotoxic activity of Staphylococcus hyicus.
abstract: Culture supernatants from a number of Staphylococcus hyicus strains caused toxic effects to both murine fibroblast and porcine keratinocyte cells in culture. The extent of cytotoxicity was shown to differ between strains and may provide an indication of strain virulence. Purification of cytotoxic activity produced by S. hyicus (strain P119) using preparative isoelectric-focussing demonstrated it to be cytolytic, haemolytic and non-proteolytic. The cytotoxin demonstrates certain properties in common with the delta haemolysin of Staphylococcus aureus.
authors.affiliation: Department of Veterinary Medicine and Animal Husbandry, Royal Veterinary College, North Mymms, Herts, UK.
authors.forename: R P
authors.initials: RP
authors.lastname: Allaker
authors.forename: M
authors.initials: M
authors.lastname: Whitlock
authors.forename: D H
authors.initials: DH
authors.lastname: Lloyd
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003603:Cytotoxins / Q000096:biosynthesis* / Q000302:isolation & purification; D005347:Fibroblasts / Q000382:microbiology; D007525:Isoelectric Focusing; D015603:Keratinocytes / Q000382:microbiology; D013210:Staphylococcus / Q000378:metabolism / Q000472:pathogenicity*; D013552:Swine; D014774:Virulence
chemical_list: D003603:Cytotoxins