12-Hydroxyeicosatetraenoic acid (12-HETE) induces heat shock proteins in human leukocytes.
12-Hydroxyeicosatetraenoic acid (12-HETE) induces the expression of individual heat shock proteins in human leukocytes (lymphocytes, monocytes, basophil granulocytes; LMBs). Metabolic radiolabeling of LMBs revealed that exogenous 12-HETE (20 ng) led to the expression of a 65- and 83kDa protein. Immunoreactivity towards the 65kDa protein was commonly detected. In contrast, after heat shock treatment and predominantly after incubation with 12-HETE significant immunoreactivity (anti-hsp72) was detected in the lower molecular weight range whereas immunoreactivity (anti-hsp90, AC88) was only observed after heat shock treatment of LMBs.
Köller
M
M
Lehrstuhl für Med. Mikrobiologie und Immunologie, Ruhr-Universität Bochum, F.R.G.
König
W
W
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D015153:Blotting, Western; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000097:blood* / Q000302:isolation & purification; D006358:Hot Temperature; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000494:pharmacology*; D066298:In Vitro Techniques; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism*; D008970:Molecular Weight
D006360:Heat-Shock Proteins; D006893:Hydroxyeicosatetraenoic Acids; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid