title: An improved method for the purification of blood cells of turkeys.
abstract: Methods were developed to purify monocytes, heterophils, or erythrocytes from the whole blood of turkeys. After the bulk of thrombocytes was removed by centrifugation, blood was fractionated over a Ficoll-Hypaque discontinuous gradient. Five fractions were harvested separately, and two were further purified by attachment to a plastic surface and/or by lysis of erythrocytes. The monocyte fraction contained 41% +/- 1% monocytes, and the heterophil fraction contained 96% +/- 1% heterophils. The erythrocyte fraction showed a purity of 99.4% +/- 0.3%. These methods would be useful for various in vitro studies that require purified populations of blood cells.
authors.affiliation: College of Veterinary Medicine, Oregon State University, Corvallis 97331.
authors.forename: J G
authors.initials: JG
authors.lastname: Strain
authors.forename: M
authors.initials: M
authors.lastname: Matsumoto
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D005591:Chemical Fractionation; D004912:Erythrocytes; D006098:Granulocytes; D008297:Male; D009000:Monocytes; D014422:Turkeys / Q000097:blood*