In vitro toxicity testing for antibacterials against human keratinocytes.
The use of cultured human keratinocytes in an in vitro comparison of topical antibacterial toxicity for epithelial cells was examined. The complement of three assessments allows testing of epithelial migration, growth, and survival. The three assessments included (1) flow cytometry for determination of cell survival, (2) a comparison of confluent cell culture growth after antibacterial exposures, and (3) an evaluation of cell migration using a technique of dermal explants to study radial migration. A comparative ranking of the toxicities of the various topical antibacterials was determined with the three assessments. This has confirmed anecdotal reports that many of the topical antibacterials are cell-toxic and may inhibit wound healing. This information can be directly extrapolated to the clinical setting, unlike many of the animal data for wound healing that currently exist.
Smoot
E C
EC
Southern Illinois University School of Medicine, Springfield.
Kucan
J O
JO
Roth
A
A
Mody
N
N
Debs
N
N
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001431:Bacteriological Techniques; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000502:physiology; D008272:Mafenide / Q000494:pharmacology; D009583:Nitrofurazone / Q000494:pharmacology; D011206:Povidone-Iodine / Q000494:pharmacology; D012835:Silver Nitrate / Q000494:pharmacology; D012837:Silver Sulfadiazine / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D012973:Sodium Hypochlorite / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D000891:Anti-Infective Agents, Local; D012965:Sodium Chloride; D008272:Mafenide; D011206:Povidone-Iodine; D012835:Silver Nitrate; D012973:Sodium Hypochlorite; D012837:Silver Sulfadiazine; D009583:Nitrofurazone