[Changes in T-lymphocyte subsets and interleukin-2 production in peripheral blood mononuclear cells after thermal injury].
The relative number of peripheral blood T-lymphocyte and its subsets, identified by monoclonal antibodies OKT3, OKT4 and OKT8, and Interleukin-2 (IL-2) were assessed in burn patients by means of indirect immunofluorescence and microassay CTLL-2 3H-TdR incorporation techniques. Comparing with the controls, the relative number of OKT3+ cell in burn patients was decreased occasionally, however, the proportion of OKT4+ cell was significantly reduced and did not recover until the patients were cured. The changes in OKT8+ cell depended on the extent of the burn wound. In minor and moderate burn patients, the proportion of OKT8+ cell was temporarily increased, while in major burn patients, it was increased persistently. The OKT4+/OKT8+ cell ratio declined constantly. The results of the experiment showed that the PBMCs IL-2 production in the patients was persistently lower than normal controls and correlated to the OKT4+/OKT8+ cell ratio. These data indicated that following burn injury, there was insufficiency of relative number in TH cells and relative dominance in Ts cells, which might affect the IL-2 production. These findings suggest that the lowered immunopotency after thermal injury may be attributed to both insufficient TH cells and excessive Ts cells.
Sun
B S
BS
Burn Unit, Ruijin Hospital, Shanghai Second Medical University.
Shi
J X
JX
D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D002056:Burns / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007376:Interleukin-2