title: Subrepeats of rDNA intergenic spacer present as prominent independent satellite DNA in Vigna radiata but not in Vigna angularis.
abstract: Subrepeats located in the rDNA intergenic spacer are also present as independently occurring, tandemly arranged satellite DNA clusters in the genome of Vigna radiata (mung bean). These 174-bp satellite repeats are identified as non-rDNA repeats by the presence of an AluI site. In the closely related Vigna angularis (adzuki bean), 174-bp repeats characterized by an AluI site occur in the rDNA with high sequence homology to the V. radiata rDNA subrepeats. A part of the 174-bp element that shows high similarity to a Xenopus terminator box (T2/T3) is slightly modified in V. angularis. However, a characteristic stem-loop structure can be formed, as in the case of V. radiata. Two highly conserved 12-bp regions occur within the 174-bp rDNA repeats of the two plants investigated. One of these 12-bp stretches exhibits some sequence identity to an element repeated twice in the 325-bp repeats in the intergenic spacer region of Vicia faba (broad bean).
authors.affiliation: Lehrstuhl für Allgemeine Genetik, Biologisches Institut, Universität Tübingen, F.R.G.
authors.forename: K
authors.initials: K
authors.lastname: Unfried
authors.forename: K
authors.initials: K
authors.lastname: Schiebel
authors.forename: V
authors.initials: V
authors.lastname: Hemleben
mesh_terms: D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics* / Q000302:isolation & purification; D004276:DNA, Satellite / Q000235:genetics* / Q000302:isolation & purification; D007887:Fabaceae / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010946:Plants, Medicinal; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity; D014158:Transcription, Genetic; D014981:Xenopus
chemical_list: D004275:DNA, Ribosomal; D004276:DNA, Satellite