TNF susceptibility-related gene expression.
A series of BWL hybridoma lines has been generated by cell fusion of a TNF-resistant BW5147 cell line and a highly TNF-sensitive WR19L cell line, and has exhibited a wide variety of TNF susceptibility. A cDNA library, BWL47rp10, from a TNF-susceptible hybridoma line BWL47 has been screened by hybridization with a 32P-labeled subtraction cDNA probe from WR19L cells. Thirty-nine candidates for TNF susceptibility were cloned, and 20 clones larger than 1.0 kb have been examined so far. A cDNA clone, 10-2, hybridized to mRNA of 1.8 kb. It was expressed higher in highly TNF-sensitive cells, especially in BWL47, BWL40, and BWL57 cells. Thus, at least, the expression of the mRNA identified with cDNA 10-2 correlated well with TNF susceptibility in BWL cells, although its functional identity remains to be determined.
Kinebuchi
T
T
Tokyo Institute for Immunopharmacology, Japan.
Nishimura
H
H
Yoshida
T
T
D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D015342:DNA Probes; D004351:Drug Resistance; D005434:Flow Cytometry; D015870:Gene Expression / Q000235:genetics; D006825:Hybridomas; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000235:genetics; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000494:pharmacology
D015342:DNA Probes; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha