Presence of circulating anti-c-myb oncogene product antibodies in human sera.
Bacterially expressed mouse c-myb protein was purified from E. coli extracts and used as antigen to screen human sera for circulating anti-c-myb oncogene product antibodies. Using Western blotting, we have found that human sera contain IgG antibodies to the c-myb gene product. The percentage of positive sera in cancer patients appears to be dependent upon cancer type and is significantly higher in breast-cancer patients than in normal donors: 43% of sera from breast-cancer patients are positive, whereas in neuroblastoma cancer patients the production of IgG anti-c-myb appears to be rare. No significant correlation was observed between the presence of circulating anti-c-myb antibodies and the expression of the c-myb gene in breast tumors.
Sorokine
I
I
Institut Jacques Monod, Université Paris VII, France.
Ben-Mahrez
K
K
Bracone
A
A
Thierry
D
D
Ishii
S
S
Imamoto
F
F
Kohiyama
M
M
D000906:Antibodies / Q000032:analysis*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000276:immunology; D003110:Colonic Neoplasms / Q000276:immunology; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D009369:Neoplasms / Q000175:diagnosis / Q000276:immunology*; D009447:Neuroblastoma / Q000276:immunology; D012516:Osteosarcoma / Q000175:diagnosis / Q000276:immunology; D010051:Ovarian Neoplasms / Q000276:immunology; D011518:Proto-Oncogene Proteins / Q000276:immunology*; D020598:Proto-Oncogene Proteins c-myb; D014407:Tumor Cells, Cultured
D000906:Antibodies; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb