title: A mutation in the large subunit ribosomal RNA gene of Tetrahymena confers anisomycin resistance and cold sensitivity.
abstract: Anisomycin, an antibiotic that specifically inhibits the peptidyl transfer function of eukaryotic ribosomes, has been used to select resistant mutants in Tetrahymena thermophila. A mutation conferring anisomycin resistance (an-r) has been localized to a 1.2-kb fragment of the large subunit ribosomal RNA (rRNA) gene by transformation via microinjection. A single base pair change was detected within this region. Nine independently isolated an-r mutants had the same base pair change. T. thermophila strains that are homozygous for this mutation are cold sensitive, unable to mate and grossly abnormal in cell morphology.
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
authors.forename: R
authors.initials: R
authors.lastname: Sweeney
authors.forename: C H
authors.initials: CH
authors.lastname: Yao
authors.forename: M C
authors.initials: MC
authors.lastname: Yao
mesh_terms: D000818:Animals; D000841:Anisomycin / Q000494:pharmacology*; D001483:Base Sequence; D003001:Cloning, Molecular; D003080:Cold Temperature; D003433:Crosses, Genetic; D003513:Cycloheximide / Q000494:pharmacology; D004275:DNA, Ribosomal / Q000235:genetics*; D004351:Drug Resistance / Q000235:genetics*; D007700:Kinetics; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D010303:Paromomycin / Q000494:pharmacology; D012335:RNA, Ribosomal / Q000235:genetics*; D013768:Tetrahymena / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development
chemical_list: D004275:DNA, Ribosomal; D015345:Oligonucleotide Probes; D012335:RNA, Ribosomal; D010303:Paromomycin; D000841:Anisomycin; D003513:Cycloheximide