Pericellular glycoconjugates of cultured fibroblasts from control and cystic fibrosis patients.
1. Labeled glycoconjugates released by trypsin from cell surfaces of control and cystic fibrosis (CF) skin fibroblasts were purified and fractionated by column chromatography on Sephadex G-50 and Concanavalin A Sepharose. Based on chemical analysis and specific enzymatic digestions: (1) Glycoconjugates were characterized as O-linked glycopeptides consisting predominantly of glycosaminoglycan type and N-linked glycopeptides with glycans of complex type. Their relative proportions were similar between the two groups. (2) The N-linked glycopeptides exhibited an increased molar ratio of fucose to galactose in CF fibroblasts. (3) When pericellular glycoconjugates were metabolically labeled with [14C]glucosamine and [3H]fucose, incorporation and degradation kinetics were similar between the two groups.
Bertrand
F
F
Laboratoire de Biochimie, I.N.S.E.R.M.-U. 181, Faculté de Médecine Saint-Antoine, Paris, France.
Hermelin
B
B
Paul
A
A
Picard
J
J
D002241:Carbohydrates / Q000032:analysis; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003550:Cystic Fibrosis / Q000378:metabolism*; D005347:Fibroblasts; D005643:Fucose / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D006001:Glycoconjugates / Q000032:analysis* / Q000302:isolation & purification; D006020:Glycopeptides / Q000302:isolation & purification; D006025:Glycosaminoglycans / Q000032:analysis / Q000302:isolation & purification; D006031:Glycosylation; D006801:Humans; D007700:Kinetics; D014357:Trypsin / Q000378:metabolism
D002241:Carbohydrates; D006001:Glycoconjugates; D006020:Glycopeptides; D006025:Glycosaminoglycans; D005643:Fucose; D014357:Trypsin; D005944:Glucosamine