title: Microcirculatory disturbances and leucocyte adherence in transplanted livers after cold storage in Euro-Collins, UW and HTK solutions.
abstract: Integrity of the hepatic microcirculation and maintenance of endothelial cell viability are critical components in preventing primary non-function after liver transplantation. Therefore, hepatic microcirculation and leucocyte-endothelial interaction were studied in rat livers stored for 1 h in Euro-Collins (EC), University of Wisconsin (UW), and histidine-tryptophan-ketoglutarate (HTK) solutions and subsequently transplanted. One hour after transplantation surgery, the livers were exposed under an intravital fluorescence microscope. After injection of the leucocyte marker acridine orange (1 mumol/kg), six pericentral fields were observed for 30 s and experiments were recorded continuously. The percentage of perfused sinusoids was reduced in the livers in the EC group (82.9%) in contrast to the UW (93.2%) and HTK groups (91.0%). Livers in the EC group showed a reduction in the diameters of pericentral sinusoids (7.3 +/- 0.2 microns; mean +/- SEM) compared with the UW group (9.5 +/- 0.2 microns; P less than 0.05) and HTK group (10.2 +/- 0.8 microns; P less than 0.05), indicating substantial cell swelling in livers stored in EC solution. Permanent adherence of leucocytes was most frequently observed in the EC group (33.5 +/- 1%), while this phenomenon was less pronounced in the UW group (14.5 +/- 1.1%; P less than 0.05) and HTK group (16.3 +/- 0.7%; P less than 0.05). Conversely, temporary adherence of leucocytes was reduced in the EC group (19.7 + 1.3%) compared with the UW group (30.5 + 2.1%) and the HTK group (34.4 + 0.8%).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Chirurgische Universitätsklinik, Homburg/Saar, Federal Republic of Germany.
authors.forename: I
authors.initials: I
authors.lastname: Marzi
authors.forename: F
authors.initials: F
authors.lastname: Walcher
authors.forename: M
authors.initials: M
authors.lastname: Menger
authors.forename: V
authors.initials: V
authors.lastname: Bühren
authors.forename: G
authors.initials: G
authors.lastname: Harbauer
authors.forename: O
authors.initials: O
authors.lastname: Trentz
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D002448:Cell Adhesion; D003080:Cold Temperature; D005260:Female; D005609:Free Radicals; D005978:Glutathione; D006639:Histidine; D006982:Hypertonic Solutions; D007328:Insulin; D007656:Ketoglutaric Acids; D007962:Leukocytes / Q000502:physiology*; D008102:Liver Circulation; D016031:Liver Transplantation; D008833:Microcirculation; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D011887:Raffinose; D051381:Rats; D011917:Rats, Inbred Lew; D012996:Solutions; D014364:Tryptophan
chemical_list: C037096:Euro-Collins' solution; D005609:Free Radicals; D006982:Hypertonic Solutions; D007328:Insulin; D007656:Ketoglutaric Acids; D019188:Organ Preservation Solutions; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D006639:Histidine; D000493:Allopurinol; D014364:Tryptophan; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose