Determination of 3-methylhistidine in hydrolysed proteins by fluorescamine derivatization and high-performance liquid chromatography.
A method is described for the determination of the 3-methylhistidine content in myofibrilar proteins (myosin and actin) using reversed-phase high-performance liquid chromatography with ultraviolet detection. Proteins were hydrolysed and free amino acids were derivatized with fluorescamine. Elution was performed isocratically with acetonitrile-water (24:76). This method allows the detection of picomole amounts of 3-methylhistidine in myosin and actin.
Dalla Libera
L
L
CNR Unit Institute of General Pathology, University of Padova, Italy.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005450:Fluorescamine / Q000737:chemistry; D006868:Hydrolysis; D008762:Methylhistidines / Q000032:analysis*; D009132:Muscles / Q000737:chemistry; D009218:Myosins / Q000737:chemistry*; D011817:Rabbits; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
D008762:Methylhistidines; D005450:Fluorescamine; D009218:Myosins; C028118:3-methylhistidine