Hepatic protein pattern alterations following perfluorodecanoic acid exposure in rats.
The effect after 8 days of 20 and 50 mg/kg in vivo exposure to perfluoro-n-decanoic acid (PFDA) on the protein pattern of rat liver whole homogenates was studied using high-resolution, large-scale two-dimensional polyacrylamide gel electrophoresis. PFDA exposure altered 13 proteins reproducibly in each of 5 samples tested. While most of the altered proteins remained unidentified, several known mitochondrial proteins were induced by PFDA as were others in a dose-related manner. Conversely, PFDA caused the reduction of albumin as well as several unknown proteins, also in a dose-related manner. Cytoskeletal proteins were unaffected by PFDA. The present results suggest that PFDA's toxic mechanism may involve more than membrane integration and lipid accumulation. Changes in the two-dimensional protein pattern indicate that selective protein synthetic or catabolic processes may undergo qualitative alterations after PFDA treatment as well.
Witzmann
F A
FA
Department of Biology, Indiana University Purdue-University at Indianapolis, Columbus 47203.
Parker
D N
DN
D000818:Animals; D003652:Decanoic Acids / Q000633:toxicity*; D015180:Electrophoresis, Gel, Two-Dimensional; D005466:Fluorocarbons / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344
D003652:Decanoic Acids; D005466:Fluorocarbons; D011506:Proteins; C036567:perfluorodecanoic acid