title: Repeats and subrepeats in the intergenic spacer of rDNA from the nematode Meloidogyne arenaria.
abstract: Ribosomal DNA (rDNA) repeats of the plant-parasitic nematode Meloidogyne arenaria are heterogeneous in size and appear to contain 5S rRNA gene sequences. Moreover, in a recA+ bacterial host, plasmid clones of a 9 kb rDNA repeat show deletion events within a 2 kb intergenic spacer (IGS), between 28S and 5S DNA sequences. These deletions appear to result from a reduction in the number of tandem 129 bp repeats in the IGS. The loss of such repeats might explain how rDNA length heterogeneity, observed in the Meloidogyne genome, could have arisen. Each 129 bp repeat also contains three copies of an 8 bp subrepeat, which has sequence similarity to an element found in the IGS repeats of some plant rDNAs.
authors.affiliation: Dept. of Biological Sciences, Simon Fraser University, Burnaby, B.C., Canada.
authors.forename: H
authors.initials: H
authors.lastname: Vahidi
authors.forename: B M
authors.initials: BM
authors.lastname: Honda
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004275:DNA, Ribosomal / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D014431:Tylenchoidea / Q000235:genetics*
chemical_list: D004275:DNA, Ribosomal