Purification and characterization of a heat-stable enterotoxin of Vibrio mimicus.
A heat-stable enterotoxin produced by Vibrio mimicus (VM-ST) was studied. VM-ST was purified from a culture supernatant of V. mimicus strain AQ-0915 by ammonium sulfate fractionation, hydroxyapatite treatment, ethanol extraction, column chromatography on both SP-Sephadex C-50 and DEAE-Sephadex A-25, and HPLC, and the recovery rate was about 15%. Purified VM-ST was heat-stable. VM-ST activity was cross-neutralized by anti-STh antiserum. The amino acid composition of the purified VM-ST was determined 17 amino acid residues in the following sequence: Ile-Asp-Cys-Cys-Glu-Ile-Cys-Cys-Asn-Pro-Ala-Cys-Phe-Gly-Cys-Leu-Asn. This composition and sequence were identical to those of V. cholerae non-O1-ST. These results clearly demonstrate the production of a characteristic VM-ST by V. mimicus.
Arita
M
M
Research Institute for Microbial Diseases, Osaka University, Japan.
Honda
T
T
Miwatani
T
T
Takeda
T
T
Takao
T
T
Shimonishi
Y
Y
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D004768:Enterotoxins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D016415:Sequence Alignment; D013696:Temperature; D014733:Vibrio / Q000378:metabolism*
D000596:Amino Acids; D004768:Enterotoxins; C069211:enterotoxin, Vibrio mimicus