Nonradioactive DNA probe for detection of gene for methicillin resistance in Staphylococcus aureus.
A DNA probe was developed by inserting, in the SmaI site of pBluescript sK, a 528-bp fragment of the gene responsible for intrinsic resistance to beta-lactam antibiotics in Staphylococcus aureus (mec determinant). The mec probe provided a useful tool for the rapid identification of the intrinsic resistance trait and for establishing guidelines for testing the in vitro susceptibility of S. aureus to beta-lactams.
Ligozzi
M
M
Institute of Microbiology, University of Verona, Italy.
Rossolini
G M
GM
Tonin
E A
EA
Fontana
R
R
D015342:DNA Probes; D006824:Hybridization, Genetic; D008712:Methicillin / Q000494:pharmacology*; D016106:Methicillin Resistance / Q000235:genetics*; D015183:Restriction Mapping; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics; D001618:beta-Lactamases / Q000378:metabolism
D015342:DNA Probes; D001618:beta-Lactamases; D008712:Methicillin