Isolation, purification and preliminary studies on fish eye lens glycoprotein.
Glycoprotein from the eye lens of fish, Mystus cavasius, was isolated by extraction with 1% Triton X-100 in saline. The crude extract which was found to be electrophoretically heterogeneous was fractionated on a DEAE-cellulose column. One of the fractions obtained in major amount was further resolved by column chromatography using Sephadex G-150 into two homogeneous fractions (GP-1 and GP-2]. GP-1 contained carbohydrates (11.2%) and protein (77.5%). The constituent sugars were D-glucose, D-mannose, D-galactosamine and N-acetyl neuraminic acid. The principal amino acids were aspartic acid, serine, glutamic acid, glycine and alanine. The proportions of these residues were determined.
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Department of Biological Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Calcutta.
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D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002848:Chromatography, DEAE-Cellulose; D005136:Eye Proteins / Q000737:chemistry / Q000302:isolation & purification*; D005399:Fishes / Q000378:metabolism*; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification*; D007908:Lens, Crystalline / Q000737:chemistry*; D017830:Octoxynol; D011092:Polyethylene Glycols
D000596:Amino Acids; D002241:Carbohydrates; D005136:Eye Proteins; D006023:Glycoproteins; D011092:Polyethylene Glycols; D017830:Octoxynol