Difference between the calcium- and the phorbol ester-induced association of protein kinase C with phospholipid membrane.
1. Membrane association of protein kinase C is thought to be a prerequisite for the activation of the enzyme. 2. We studied the association of the enzyme with liposome. 3. We show here that the mechanisms for the calcium- and the phorbol ester-induced association of protein kinase C with liposome are different from each other. 4. Diacylglycerol is not crucial for the association of the enzyme with liposome.
Irita
K
K
Glaxo Research Laboratories, Research Triangle Park, NC 27709.
McConnell
R
R
Cuatrecasas
P
P
Hebdon
G M
GM
D000818:Animals; D001921:Brain / Q000201:enzymology; D002118:Calcium / Q000494:pharmacology*; D004075:Diglycerides / Q000494:pharmacology; D004357:Drug Synergism; D008081:Liposomes / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D010713:Phosphatidylcholines / Q000378:metabolism; D010718:Phosphatidylserines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D004075:Diglycerides; D008081:Liposomes; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D010743:Phospholipids; D011189:Potassium Chloride; D011493:Protein Kinase C; D008274:Magnesium; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium