title: Purification and characterization of a new heat-stable enterotoxin produced by Vibrio cholerae non-O1 serogroup Hakata.
abstract: The possible production of a heat-stable enterotoxin (Vc-H-ST) by Vibrio cholerae non-O1 serogroup Hakata was investigated, and the purified Vc-H-ST was characterized. It has a unique amino acid sequence, LIDCCEICCNPACFGCLN. This sequence is quite similar to that of the heat-stable enterotoxin (NAG-ST) produced by V. cholerae non-O1 except for one amino acid (leucine) residue excess at the N terminus. Other characteristics, including biological activity, are compatible with those of NAG-ST.
authors.affiliation: Research Institute for Microbial Diseases, Yamadaoka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Arita
authors.forename: T
authors.initials: T
authors.lastname: Honda
authors.forename: T
authors.initials: T
authors.lastname: Miwatani
authors.forename: K
authors.initials: K
authors.lastname: Ohmori
authors.forename: T
authors.initials: T
authors.lastname: Takao
authors.forename: Y
authors.initials: Y
authors.lastname: Shimonishi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004768:Enterotoxins / Q000737:chemistry / Q000302:isolation & purification*; D051379:Mice; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D012703:Serotyping; D014734:Vibrio cholerae / Q000145:classification / Q000378:metabolism*
chemical_list: D004768:Enterotoxins; C037420:stN protein, Vibrio cholerae