Specific hybridization probes for mouse type I, II, III and IX collagen mRNAs.
We have constructed DNA probes for the specific detection of mouse pro alpha 1(I), pro alpha 1(II), pro alpha 1(III) and alpha 1(IX) collagen transcripts. To avoid cross-hybridization the probes for fibrillar collagens cover mainly sequences in the 3' untranslated region of the gene. Sequencing and Northern analysis confirmed that the clones share minimal sequence similarity and detect only the specific mRNAs under normal hybridization and washing conditions. The clone for mouse alpha 1(IX) collagen covers coding sequences but is sufficiently divergent from other collagen transcripts to allow specific detection of the corresponding mRNA.
Metsäranta
M
M
University of Texas M.D. Anderson Cancer Center, Department of Molecular Genetics, Houston.
Toman
D
D
De Crombrugghe
B
B
Vuorio
E
E
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003094:Collagen / Q000235:genetics*; D015342:DNA Probes; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics*; D014158:Transcription, Genetic
D015342:DNA Probes; D012333:RNA, Messenger; D003094:Collagen