Induction of hepatic cytochrome P-450 and drug metabolism by doxapram in the mouse.
The ability of doxapram (20 mg/kg, i.p. daily for 5 days) to induce hepatic cytochrome P-450 and xenobiotic metabolism was examined in male mice. Compared with the control values, the doxapram treatment significantly increased the amount of cytochrome P-450, and activities of aminopyrine N-demethylase, ethylmorphine N-demethylase and meperidine N-demethylase. In contrast, there were no changes in the activities of aniline hydroxylase, 7-ethoxycoumarine deethylase, NADPH-cytochrome c reductase and NADH-ferricyanide reductase, and cytochrome b5 content. These data show that there is a substrate specificity in the ability of doxapram to induce the hepatic drug metabolism in male mice.
Ishikawa
M
M
Department of Pharmacology and Toxicology, Cancer Research Institute, Tohoku College of Pharmacy, Komatsushima Aobaku, Sendai, Japan.
Ozaki
M
M
Takayanagi
Y
Y
Sasaki
K
K
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004315:Doxapram / Q000494:pharmacology*; D004790:Enzyme Induction; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051379:Mice; D006899:Mixed Function Oxygenases / Q000096:biosynthesis*; D015262:Xenobiotics / Q000378:metabolism*
D015262:Xenobiotics; D003577:Cytochrome P-450 Enzyme System; D004315:Doxapram; D006899:Mixed Function Oxygenases