Immunocytochemical identification of nuclear estrogen-receptors in human acute myeloid leukemia.
The peripheral and bone marrow blast cells of twenty patients newly-diagnosed as acute myeloid leukemia (AML), subtypes M1-M5, were analysed for the presence of estrogen-receptors by ER-ICA immunocytochemistry and dextran-coated charcoal cytosolic (DCC) assay. Two patients demonstrated myeloblasts with nuclear staining patterns consistent with the presence of estrogen-receptors. The positive immuno-staining results were confirmed by DCC in one patient with AML, subtype M4. This patient demonstrated an unusual myeloblast karyotype with a reciprocal translocation t(6;11)(q27;q23.3) involving the designated locus of the ESR gene on chromosome 6 (6q24-qter). The abnormal juxtaposition of DNA control elements close to the promoter of the ESR gene may be one mechanism by which aberrant synthesis of estrogen-receptor protein can occur to provide a growth advantage for leukemia cell clones.
Hatfill
S J
SJ
Department of Haematology Pathology, Faculty of Medicine, University of Stellenbosch, Tygerberg, Republic of South Africa.
Brusnicky
J
J
Fester
E
E
D000208:Acute Disease; D000906:Antibodies; D000911:Antibodies, Monoclonal; D001853:Bone Marrow / Q000473:pathology*; D002467:Cell Nucleus / Q000648:ultrastructure*; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D025063:Chromosome Disorders; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D007938:Leukemia / Q000097:blood* / Q000235:genetics / Q000473:pathology; D011960:Receptors, Estrogen / Q000032:analysis*; D014178:Translocation, Genetic
D000906:Antibodies; D000911:Antibodies, Monoclonal; D011960:Receptors, Estrogen