Dexamethasone and 1,25(OH)2 vitamin D3 modulate the synthesis of insulin-like growth factor-I in osteoblast-like cells.
In the present study, we have shown that insulin-like growth factor-I (IGF-I) was released by primary cultures of rat osteoblast-like (ROB) cells into the conditioned medium (CM). Dexamethasone (DEX) caused a dose-dependent inhibition of the IGF-I. At 10(-8) M, DEX reduced IGF-I level to 70% of the control value (P less than 0.05); at 10(-7) M DEX, the IGF-I level was further reduced to 60% of the control (P less than 0.01). The active vitamin D metabolite 1,25-dihydroxycholecalciferol [1,25(OH)2D3] slightly increased the IGF-I level, but the increase was not statistically significant. However, in combined treatments of 10(-7) M DEX and 10(-8) M of 1,25(OH)2D3, the inhibition of DEX was partially antagonized by the presence of 1,25(OH)2D3. Studies with metabolically radiolabeled IGF-I by immunoprecipitation indicated the changes of IGF-I in the CM reflected synthesis of the protein by the cells. The alteration of IGF-I level may mediate some of the actions of these steroid hormones on bone cells.
Chen
T L
TL
California Biotechnology Inc., Mountain View 94043.
Mallory
J B
JB
Hintz
R L
RL
D000818:Animals; D002117:Calcitriol / Q000494:pharmacology*; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D010006:Osteoblasts / Q000378:metabolism*; D011233:Precipitin Tests; D011863:Radioimmunoassay; D051381:Rats
D007334:Insulin-Like Growth Factor I; D003907:Dexamethasone; D002117:Calcitriol