[Multi-center evaluation of Showa disk susceptibility to presumptively determine minimum inhibitory concentrations through linear regression analysis].
To confirm the reliability of minimum inhibitory concentrations (MICs) determined by use of predefined linear regressions to bacterial growth inhibitory zone diameter on Showa disk susceptibility test, the multi-center evaluation along daily routines was performed in comparison with the standard agar dilution method. In total, 4,107 (89.0%) of 4,613 testings gave comparable MICs with 4-fold or less differences to those determined by the standard agar dilutions. The agreement of MICs (less than or equal to 4-fold differences) for gram-negative rods, excluding Pseudomonas aeruginosa, against 10 antimicrobial agents was estimated to 92.1%, and those for gram-positive cocci against 9 agents and for the strains of Pseudomonas aeruginosa against 8 agents were 84.5% and 81.7%, respectively. With these results, we can conclude that, under the well-controlled test procedures, the MIC correlates determined by Showa disk susceptibility test are enough comparable to those determined by the standard agar dilution method.
Sato
Y
Y
Department of Laboratory Medicine, Kumamoto University Medical School.
Nagata
K
K
Tasaki
Y
Y
Tosaka
M
M
Yamane
N
N
D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development; D005069:Evaluation Studies as Topic; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000592:standards*; D011237:Predictive Value of Tests; D012044:Regression Analysis
D000900:Anti-Bacterial Agents