Structural and functional conservation of histidinol dehydrogenase between plants and microbes.
The partial amino acid sequence of histidinol dehydrogenase (L-histidinol:NAD+ oxidoreductase, EC 1.1.1.23) from cabbage was determined from peptide fragments of the purified protein. The relative positions of these peptides were deduced by aligning their sequences with the sequence of the HIS4C gene product of Saccharomyces cerevisiae. cDNA encoding histidinol dehydrogenase was then amplified from a library using a polymerase chain reaction primed with degenerate oligonucleotide pools of known position and orientation. By using this amplified fragment as a probe, an apparently full-length cDNA clone was isolated that is predicted to encode a proenzyme having a putative 31-amino acid chloroplast transit peptide and a mature molecular mass of 47.5 kDa. The predicted protein sequence was 51% identical to the yeast enzyme and 49% identical to the Escherichia coli enzyme. Expression of the cDNA clone in an E. coli his operon deletion strain rendered the mutant able to grow in the presence of histidinol.
Nagai
A
A
International Research Laboratories, CIBA-GEIGY Ltd., Takarazuka, Japan.
Ward
E
E
Beck
J
J
Tada
S
S
Chang
J Y
JY
Scheidegger
A
A
Ryals
J
J
D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D001937:Brassica; D003062:Codon; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D015870:Gene Expression; D008969:Molecular Sequence Data; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010446:Peptide Fragments / Q000737:chemistry; D010944:Plants / Q000201:enzymology* / Q000235:genetics; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D003062:Codon; D010446:Peptide Fragments; D004247:DNA; D000429:Alcohol Oxidoreductases; C021358:histidinol dehydrogenase