Fibroblast stimulation by monocytes cultured on protein adsorbed biomedical polymers. I. Biomer and polydimethylsiloxane.
The studies presented in this paper evaluate the modulatory role of protein pre-adsorbed polydimethylsiloxane (PDMS) and Biomer on the secretion of fibroblast stimulating growth factors from human monocytes/macrophages. The results of these studies show that Biomer and PDMS selectively activate human monocytes to produce fibroblast "progression-like" and to a lesser extent "competence-like" stimulating growth factors. Polydimethylsiloxane stimulated the monocytes/macrophages to produce more "progression-like" fibro-blast stimulating growth factors than Biomer. The induction of "competence-like" fibroblast stimulating activity from the monocytes was enhanced by preadsorption of PDMS with human derived fibrinogen, fibronectin, IgG, hemoglobin, or albumin. This phenomenon was not observed with protein pre-adsorbed Biomer. These studies support the hypothesis that protein pre-adsorbed polymers will selectively modulate monocyte/macrophage activation and induction of growth factors which have the potential to participate in tissue-implant interactions in vivo.
Bonfield
T L
TL
Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106.
Colton
E
E
Anderson
J M
JM
D000328:Adult; D001672:Biocompatible Materials; D002450:Cell Communication; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D004261:DNA Replication; D004129:Dimethylpolysiloxanes; D005333:Fetus; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D006133:Growth Substances / Q000096:biosynthesis; D006801:Humans; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000502:physiology*; D011140:Polyurethanes; D012828:Silicones; D013936:Thymidine / Q000378:metabolism
D001672:Biocompatible Materials; D004129:Dimethylpolysiloxanes; D006133:Growth Substances; D011140:Polyurethanes; D012828:Silicones; C031892:biomer; C013830:baysilon; D013936:Thymidine