[In vitro effect of shen-xue-ling on megakaryocytopoiesis in patients with idiopathic thrombocytopenic purpura].
This paper, used an improved methylcellulose-agar double layer method in vitro to culture megakaryocyte progenitors (CFU-meg) of 15 normal bone marrow donors and 20 idiopathic thrombocytopenic purpura (ITP) patients bone marrow cells in order to observe the action of Shen-Xue-Ling (SXL). The results showed that the colony efficiency of CFU-meg of ITP group (13.75 +/- 6.93/10(5) MNC) was obviously lower than that of the control group (21.22 +/- 9.06/10(3) MNC, P less than 0.01). The serum of the patients whose PAIgG was markedly higher than normal value inhibited growth of CFU.meg derived from normal bone marrow cells (P less than 0.01). SXL could increase the colony efficiency of CFU-meg for ITP patients at several doses. The average colonies were 15.77 +/- 6.31/10(5) MNC (at 100 mg/ml, P greater than 0.05), 18.72 +/- 5.54/10(5) MNC (at 200 mg/ml, P less than 0.05), 21.60 +/- 7.35/10(5) MNC (at 300 mg/ml, P less than 0.01) and 21.84 +/- 7.15/10(5) MNC (at 500 mg/ml, P less than 0.01) as compared with the group of ITP patients. Incubated with patient's serum (1%) and SXL (at 300 mg/ml), the number of CFU-meg extracted from normal bone marrow cells was significantly higher than that from patients (P less than 0.01) and was close to normal group (P greater than 0.05). The results suggested that SXL might inhibit the antiplatelet antibodies, facilitate reproduction, division and maturity of CFU-meg.
Zhou
Y M
YM
Yueyang Hospital Affiliated to Shanghai College of TCM.
Xue
Z Z
ZZ
Huang
Z Q
ZQ
D000293:Adolescent; D000328:Adult; D001853:Bone Marrow / Q000473:pathology; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000473:pathology; D006801:Humans; D008297:Male; D008533:Megakaryocytes / Q000187:drug effects* / Q000473:pathology; D008875:Middle Aged; D011696:Purpura, Thrombocytopenic / Q000473:pathology*
D004365:Drugs, Chinese Herbal