In vitro response of peripheral blood mononuclear cells to phytohemagglutinin and interleukin-2.
The serological determination of class II antigens is still a mandatory test prior to allotransplantation. It is known that these antigens are normally expressed on B lymphocytes and monocytes. The B lymphocytes that constitute 10% to 15% of total blood lymphocytes are the cells currently used for HLA-DR typing. To avoid HLA-DR typing difficulties, or even impossibilities that are frequently encountered among some patient groups, we studied the response of peripheral blood mononuclear cells--as an alternative source of cells for class II antigen typing--to in vitro mitogen and interleukin-2 activation and propagation. Although the patients included in this study were selected having previously known HLA-DR typing difficulties, all could be adequately typed by this method.
el-Barrawy
M
M
Department of Immunology and Blood Bank, M.D. Anderson Cancer Center, Texas University, Houston 77030.
Lichtiger
B
B
Fisher
H
H
Daryl
O
O
D001402:B-Lymphocytes / Q000276:immunology; D006684:HLA-DR Antigens / Q000145:classification*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008213:Lymphocyte Activation; D010835:Phytohemagglutinins / Q000494:pharmacology; D014181:Transplantation Immunology
D006684:HLA-DR Antigens; D007376:Interleukin-2; D010835:Phytohemagglutinins