Dendritic cells in the murine dermis in delayed-type contact hypersensitivity. An ultrastructural and immunocytochemical study.
Dendritic cells within the dermis in the later stages of delayed-type contact hypersensitivity were examined ultrastructurally and immunohistochemically. The immunohistochemical observations were done using monoclonal antibody M1-8, which reacts specifically with murine Langerhans cells and interdigitating cells. Seventeen hours after challenge, infiltrating cells in the dermis included dendritic cells, possibly so-called indeterminate cells, monocytoid cells and Langerhans cells. Immunohistochemically, the indeterminate cells and some monocytoid cells were M1-8-positive. These findings suggest that indeterminate cells are intimately related to Langerhans cells, and that they belong to the mononuclear phagocyte system. M1-8 is a very useful marker for studies on the kinetics of Langerhans cells or indeterminate cells.
Kuwabara
H
H
Second Department of Pathology, Kagawa Medical School, Japan.
Uda
H
H
Saito
K
K
Maruyama
T
T
Tanaka
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002453:Cell Cycle / Q000502:physiology; D002465:Cell Movement; D003713:Dendritic Cells / Q000276:immunology / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D006968:Hypersensitivity, Delayed / Q000276:immunology / Q000473:pathology* / Q000503:physiopathology; D007150:Immunohistochemistry; D007801:Langerhans Cells / Q000276:immunology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D012867:Skin / Q000276:immunology / Q000648:ultrastructure*
D000911:Antibodies, Monoclonal