Immunohistochemical investigation in odontogenic myxoma.
Three odontogenic myxomas are described immunohistochemically by a panel of poly- and monoclonal antibodies to characterize this tumor type. Three types of odontogenic myxoma cells were discriminated: spindle cells, stellate cells and hyaline cells. Neoplastic cells of myxomas were positively stained for transferrin, ferritin, alpha-1-antichymotrypsin (alpha 1-ACT), alpha-1-antitrypsin (alpha 1-AT), S-100 protein and vimentin; however, neuron specific enolase (NSE), S-100 alpha subunit, S-100 beta subunit, Factor VIII-related antigen (FVIII-AG) and cytokeratin (CK1) were negative. Spindle cells were positive for transferrin, ferritin, alpha 1-ACT, alpha 1-AT, S-100 protein and vimentin. Stellate cells were strongly positive for transferrin, alpha 1-AT, S-100 protein and vimentin. Hyaline cells reacted with alpha 1-ACT and alpha 1-AT. Myxomatous matrix showed negative reaction for all the antibodies used. These results have confirmed that odontogenic myxoma is a tumor of a dual fibroblastic-histiocytic origin.
Takahashi
H
H
Department of Oral Pathology, Nagasaki University School of Dentistry, Japan.
Fujita
S
S
Okabe
H
H
D000293:Adolescent; D014408:Biomarkers, Tumor / Q000032:analysis*; D002467:Cell Nucleus / Q000648:ultrastructure; D002648:Child; D003593:Cytoplasm / Q000648:ultrastructure; D005260:Female; D005293:Ferritins / Q000032:analysis*; D005347:Fibroblasts / Q000737:chemistry; D006644:Histiocytes / Q000737:chemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D009232:Myxoma / Q000737:chemistry / Q000473:pathology; D009808:Odontogenic Tumors / Q000737:chemistry* / Q000473:pathology; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D009418:S100 Proteins / Q000032:analysis; D014168:Transferrin / Q000032:analysis*; D014746:Vimentin / Q000032:analysis; D000514:alpha 1-Antichymotrypsin / Q000032:analysis*; D000515:alpha 1-Antitrypsin / Q000032:analysis*
D014408:Biomarkers, Tumor; D009418:S100 Proteins; D014168:Transferrin; D014746:Vimentin; D000514:alpha 1-Antichymotrypsin; D000515:alpha 1-Antitrypsin; D005293:Ferritins; D010751:Phosphopyruvate Hydratase