Immunological relationships between the subunits of textilotoxin and rabbit antisera raised against textilotoxin and some snake venoms.
The binding of textilotoxin and its subunits A, B, C and D to polyclonal rabbit antisera directed against textilotoxin, Australian common brown snake (Pseudonaja textilis) and tiger snake (Notechis scutatus scutatus) venoms was studied by ELISA. Subunit D showed greatest reactivity with antisera to textilotoxin and brown snake venom. The phospholipase A2 active subunit A reacted more strongly with antisera to tiger snake venom in keeping with the high degree of homology between the amino acid sequences of subunit A and notexin from tiger snake venom. Subunit A was the only subunit lethal to mice, but at doses 1000-fold greater than for textilotoxin.
Pearson
J A
JA
Department of Biological Sciences, Deakin University, Geelong, Victoria, Australia.
Tyler
M I
MI
Howden
M E
ME
D000818:Animals; D004546:Elapid Venoms / Q000276:immunology* / Q000633:toxicity; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera / Q000276:immunology*; D051379:Mice; D011817:Rabbits
D004546:Elapid Venoms; D007106:Immune Sera; C037525:textilotoxin