title: Linear order of new and established DNA markers around the fragile site at Xq27.3.
abstract: We have used recombinant clones derived from microdissection of the fragile X region to characterize breakpoints around the fragile site at Xq27.3. So far, no microdissection markers derived from Xq28 material have been found, thus allowing a rapid screening for clones surrounding the fragile site by their presence in a somatic cell hybrid containing Xq27.2-Xqter. A total of 43 new DNA markers from Xq27 have been sublocalized within this chromosome band. Of these new DNA markers, 5 lie in an interval defined as containing the fragile X region. The saturation of Xq27 with DNA markers by microdissection demonstrates the power of this technique and provides the resources for generating a complete physical map of the region.
authors.affiliation: Molecular Genetics Group, John Radcliffe Hospital, Headington, Oxford, United Kingdom.
authors.forename: M C
authors.initials: MC
authors.lastname: Hirst
authors.forename: A
authors.initials: A
authors.lastname: Roche
authors.forename: T J
authors.initials: TJ
authors.lastname: Flint
authors.forename: R N
authors.initials: RN
authors.lastname: MacKinnon
authors.forename: J H
authors.initials: JH
authors.lastname: Bassett
authors.forename: Y
authors.initials: Y
authors.lastname: Nakahori
authors.forename: J E
authors.initials: JE
authors.lastname: Watson
authors.forename: M V
authors.initials: MV
authors.lastname: Bell
authors.forename: M N
authors.initials: MN
authors.lastname: Patterson
authors.forename: Y
authors.initials: Y
authors.lastname: Boyd
mesh_terms: D015139:Blotting, Southern; D002460:Cell Line; D003001:Cloning, Molecular; D005600:Fragile X Syndrome / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics*; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction; D014960:X Chromosome
chemical_list: D005819:Genetic Markers