title: Mutations permitting the anaerobic growth of Escherichia coli on trehalose.
abstract: Wild-type strains of Escherichia coli K-12 do not grow anaerobically on trehalose or galactose. We isolated two operon fusion mutants of E. coli which gained the ability to grow on trehalose anaerobically (tan). The tanA-lac mutation was located at 41 min on the E. coli genetic map and also abolished growth on glucuronic acid both aerobically and anaerobically. The tanB-lac mutation was mapped to 68 min and permitted anaerobic growth on galactose as well as trehalose. The tanB-lac fusion was induced anaerobically whereas tanA-lac showed more or less constitutive beta-galactosidase expression.
authors.affiliation: Department of Microbiology, Southern Illinois University, Carbondale 62901.
authors.forename: F
authors.initials: F
authors.lastname: Mat-Jan
authors.forename: C R
authors.initials: CR
authors.lastname: Williams
authors.forename: D P
authors.initials: DP
authors.lastname: Clark
mesh_terms: D000693:Anaerobiosis; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D009154:Mutation; D009876:Operon; D014161:Transduction, Genetic; D014199:Trehalose / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D014199:Trehalose; D005690:Galactose