title: Evidence that nebulin is a protein-ruler in muscle thin filaments.
abstract: Partial amino acid sequence was obtained from the massive myofibrillar protein nebulin. This consists of repeating motifs of about 35 residues and super-repeats of 7 x 35 = 245 residues. The repeat-motifs are likely to be largely alpha-helical and to interact with both actin and tropomyosin in thin filaments. Nebulin from different species was found to vary in size in proportion to filament length. The data are consistent with the proposal that nebulin acts as a protein-ruler to regulate precise thin filament assembly.
authors.affiliation: European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Labeit
authors.forename: T
authors.initials: T
authors.lastname: Gibson
authors.forename: A
authors.initials: A
authors.lastname: Lakey
authors.forename: K
authors.initials: K
authors.lastname: Leonard
authors.forename: M
authors.initials: M
authors.lastname: Zeviani
authors.forename: P
authors.initials: P
authors.lastname: Knight
authors.forename: J
authors.initials: J
authors.lastname: Wardale
authors.forename: J
authors.initials: J
authors.lastname: Trinick
mesh_terms: D008841:Actin Cytoskeleton / Q000648:ultrastructure; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002645:Chickens; D004247:DNA / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000737:chemistry / Q000502:physiology*; D011817:Rabbits
chemical_list: D009124:Muscle Proteins; C035620:nebulin; D004247:DNA