Radiation damage in soft X-ray microscopy of live mammalian cells.
When specimens are observed by soft X-ray microscopy, they always absorb many photons, causing radiation damage at the imaged site. The problems of radiation damage were studied in view of the principle of image formation; absorption contrast, scattering (holography), or phase contrast. In all cases, photons with a wavelength of 1-10 nm interact with the specimen mainly through the photoelectric effect followed by the transfer of energy to the imaging site either directly (absorption imaging) or indirectly (holography or phase contrast). This absorbed energy will cause structural changes to the imaging site. From a review of the literature the absorbed dose is estimated to be as high as 10(7) Gy when the expected resolution of the specimen (1-10 microns thick) is 10 nm. This dose is far in excess of the amount required for cells to be able to survive when live mammalian cells are exposed. The levels of radiation effects were extrapolated to the estimated absorbed dose from the reported values for cell survival, chromosome aberrations, and DNA strand breaks with respect to observations on mammalian chromosomes. The extrapolated results show that some damage will occur in every 10 x 10-nm (expected resolution) size unit. Although these studies focused only on the effects on mammalian chromosomes, the present results are more or less common phenomena in the observation of biological specimens. Hence, the results suggest that dynamic observations will be difficult. On the other hand, a time-scale study of the effects of radiation on structural integrity suggests that single-shot imaging with short-pulsed (probably shorter than a few milliseconds) X-rays may be appropriate for the observation of intact live biological specimens in the hydrated condition, before they have deteriorated.
Shinohara
K
K
Department of Radiation Research, Tokyo Metropolitan Institute of Medical Science, Japan.
Ito
A
A
D002470:Cell Survival / Q000528:radiation effects; D002477:Cells / Q000528:radiation effects / Q000648:ultrastructure*; D002869:Chromosome Aberrations; D004247:DNA / Q000528:radiation effects; D004249:DNA Damage; D006801:Humans; D066298:In Vitro Techniques; D008853:Microscopy / Q000379:methods*; D013997:Time Factors; D014965:X-Rays
D004247:DNA