DNA fingerprinting by pulsed field gel electrophoresis and ribotyping to distinguish Pseudomonas cepacia isolates from a nosocomial outbreak.
We typed 40 isolates of Pseudomonas cepacia obtained from patients involved in a single outbreak using pulsed field gel electrophoresis and ribotyping. All isolates from the majority of the patients, 16 of 18 (89%), were included in a single group. These typing methods should aid in the clarification of the epidemiology of infection with P. cepacia.
Anderson
D J
DJ
Department of Medicine, University of Minnesota School of Medicine, Minneapolis 55455.
Kuhns
J S
JS
Vasil
M L
ML
Gerding
D N
DN
Janoff
E N
EN
D015373:Bacterial Typing Techniques; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D005069:Evaluation Studies as Topic; D006801:Humans; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D011552:Pseudomonas Infections / Q000453:epidemiology* / Q000382:microbiology
D004269:DNA, Bacterial