Thermolysin: a peptide forming enzyme.
Thermolysin, a thermostable endopeptidase, is recognised as a potential peptide bond forming enzyme. The importance of structural properties and its stereospecific nature towards peptide bond formation is described. Thermolysin's use in the keystep of the preparation of an artificial sweetener 'aspartame' is highlighted.
Reddy
A V
AV
Vittal Mallya Scientific Research Foundation, Bangalore, India.
D000595:Amino Acid Sequence; D001218:Aspartame / Q000378:metabolism*; D001407:Bacillus / Q000201:enzymology*; D002118:Calcium / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism; D008666:Metalloendopeptidases / Q000378:metabolism; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000378:metabolism*; D013379:Substrate Specificity; D013820:Thermolysin / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism
D009842:Oligopeptides; D010450:Endopeptidases; D008666:Metalloendopeptidases; D013820:Thermolysin; D015032:Zinc; D002118:Calcium; D001218:Aspartame