title: Failure of allopurinol to protect against cerebral injury when given after the start of hypoxia.
abstract: One cause of ischemic brain injury is free radical formation during recirculation. Allopurinol inhibits xanthine oxidase, an important source of free oxygen radicals. It is known that allopurinol pre-treatment has a protective action during cerebral ischemia. In the present study we exposed slices from the rat hippocampus to 9 minutes of hypoxia to test whether it is sufficient that allopurinol is present in the tissue at the time of reoxygenation. Forty-six slices loaded with allopurinol (10(-5) M) prior to reoxygenation (during hypoxia) were compared to 34 control slices. The response of the pyramidal cell population to orthodromic stimulation was reduced in both groups and there was not a significant difference between the two groups.
authors.affiliation: Institute of Surgical Research, National Hospital, Rikshospitalet, Oslo, Norway.
authors.forename: E
authors.initials: E
authors.lastname: Hegstad
authors.forename: J
authors.initials: J
authors.lastname: Berg-Johnsen
authors.forename: I A
authors.initials: IA
authors.lastname: Langmoen
mesh_terms: D000493:Allopurinol / Q000494:pharmacology*; D000818:Animals; D046508:Culture Techniques; D005071:Evoked Potentials / Q000187:drug effects / Q000502:physiology; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D015424:Reperfusion
chemical_list: D000493:Allopurinol