New classes of Streptomyces coelicolor A3(2) mutants blocked in undecylprodigiosin (Red) biosynthesis.
Fifteen mutants of Streptomyces coelicolor A3(2) blocked in both the bipyrrole branch (redA) and a second site specific to the undecylprodigiosin pathway were characterized. Some of the mutants were ordered biosynthetically based on cosynthesis experiments. Complementation of each of the mutants with wild-type DNA cloned in low- and high-copy number plasmid vectors allowed the mutants to be separated into 12 new classes which are physically clustered within approximately 37 kb on the S. coelicolor genome. Early-step biosynthetic genes are centrally located and are flanked by later-step and regulatory genes.
Coco
E A
EA
Medical Research Division, American Cyanamid Company, Pearl River, NY 10965.
Narva
K E
KE
Feitelson
J S
JS
D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005816:Genetic Complementation Test; D009154:Mutation; D010957:Plasmids; D011353:Prodigiosin / Q000031:analogs & derivatives* / Q000096:biosynthesis; D013302:Streptomyces / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
D004269:DNA, Bacterial; C047347:undecylprodigiosin; D011353:Prodigiosin