The repair of DNA damages induced in normal human skin fibroblasts exposed to simulated sunlight.
The induction and repair of DNA damages produced by exposure of normal human skin fibroblasts to the simulated sunlight produced by a solar simulator were examined. The photoproducts measured were pyrimidine dimers, E. coli endonuclease III-sensitive sites, 6-4 photoproducts, Dewar isomers, DNA-protein crosslinks, and DNA single-strand breaks. The results of these experiments serve to form a basis for the quantitation of damages induced by exposure to sunlight.
Rosenstein
B S
BS
Department of Radiation Medicine, Brown University, Providence, Rhode Island 02912.
Mitchell
D L
DL
D000328:Adult; D002478:Cells, Cultured; D004249:DNA Damage; D004260:DNA Repair; D004277:DNA, Single-Stranded / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000032:analysis; D043224:Deoxyribonuclease (Pyrimidine Dimer); D004706:Endodeoxyribonucleases; D005260:Female; D005347:Fibroblasts / Q000528:radiation effects*; D006801:Humans; D011740:Pyrimidine Dimers / Q000378:metabolism; D012867:Skin / Q000528:radiation effects*; D014466:Ultraviolet Rays
D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D011740:Pyrimidine Dimers; D004706:Endodeoxyribonucleases; D043224:Deoxyribonuclease (Pyrimidine Dimer)