A versatile solid support system for oligodeoxynucleotide probe-based hybridization assays.
A procedure for immobilization of well-defined quantities of oligodeoxyribonucleotides (ODNs) to a versatile nylon support is described. The solid support, a nylon-6/6 bead, is covalently coated with poly(ethyleneimine) to provide a reactive spacer-arm for attachment of ODNs. 5'-Aminohexyl-tailed ODNs are selectively activated using 2,4,6-trichloro-1,3,5-triazine (cyanuric chloride) and then covalently attached to the bead via the triazine moiety. The modified nylon support has a low level of binding of nonspecific nucleic acid and efficiently captures both RNA and DNA targets.
Van Ness
J
J
MicroProbe Corporation, Bothell, WA 98021.
Kalbfleisch
S
S
Petrie
C R
CR
Reed
M W
MW
Tabone
J C
JC
Vermeulen
N M
NM
D000478:Alkylation; D001483:Base Sequence; D003124:Colorimetry; D004247:DNA; D005453:Fluorescence; D005821:Genetic Techniques; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009757:Nylons; D015345:Oligonucleotide Probes; D011094:Polyethyleneimine; D014227:Triazines
D009757:Nylons; D015345:Oligonucleotide Probes; D014227:Triazines; C019309:cyanuric chloride; D011094:Polyethyleneimine; D004247:DNA