Detection of immunoglobulin G antibodies to cholesterol in antisera to mycoplasmas.
Antisera were produced by inoculation of mycoplasma cells grown in PPLO broth supplemented with rabbit serum alone or rabbit serum plus Freund complete or incomplete adjuvant. By using an enzyme-linked immunosorbent assay, immunoglobulin G antibodies to cholesterol were detected in antisera to mycoplasmas (Mycoplasma salivarium ATCC 23064, M. orale ATCC 15539, M. buccale IID 802, M. faucium IID 996, and M. hominis IID 801) and rabbit serum.
Watanabe
T
T
Department of Oral Bacteriology, Hokkaido University School of Dentistry, Sapporo, Japan.
Kumazawa
T
T
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D002784:Cholesterol / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000032:analysis*; D009174:Mycoplasma / Q000276:immunology*; D011817:Rabbits
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D002784:Cholesterol