[Changes in intra-renal scavenging enzymes activities of the reactive oxygen species in experimental glomerulonephritis and nephrosis in rats].
The purpose of this study was to investigate the changes in scavenging enzymes activities of reactive oxygen species (ROS) in experimental nephritis and nephrosis. Anti-GBM nephritis was induced in rat by injecting 0.75 ml of the anti-GBM serum. Intrarenal SOD-like activity in nephritic rats was significantly decreased from the 5th day after i.v. injection of antiserum to the 10th day. Although catalase and glutathione peroxidase activities in nephritic rat were increased on 3hr to 6hr after i.v. injection of antiserum, the both activities were gradually decreased on 24hr onward. The decrease in ROS scavenging enzymes activities paralleled with the urinary protein excretion and the elevation of plasma lipid peroxide. Moreover, catalase, administered (4,600 U/hr) by osmotic minipump prevented the urinary protein excretion by about 60%. In accelerated passive Heymann nephritis and puromycin aminonucleoside nephrosis in rats, the activities of scavenging enzymes decreased in the course of experimental periods. It was concluded that the scavenging enzymes activities of ROS increased at beginning of heterologous phase and decreased at the end of this phase in anti-GBM nephritis, and the activities in the other models of experimental nephritis also decreased during experimental periods.
Hattori
T
T
Department of Pharmacology, Faculty of Pharmacy, Meijo University, Nagoya, Japan.
Ito
M
M
Suzuki
Y
Y
D000818:Animals; D002374:Catalase / Q000378:metabolism*; D004195:Disease Models, Animal; D016166:Free Radical Scavengers; D005921:Glomerulonephritis / Q000201:enzymology*; D005979:Glutathione Peroxidase / Q000378:metabolism*; D007672:Kidney Cortex / Q000201:enzymology*; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D009401:Nephrosis / Q000201:enzymology*; D011507:Proteinuria; D051381:Rats; D011919:Rats, Inbred Strains; D013482:Superoxide Dismutase / Q000378:metabolism*
D016166:Free Radical Scavengers; D008054:Lipid Peroxides; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase