[Modification of in vitro hematopoiesis induced by addition of GM-CSF in long term normal human bone marrow cultures].
We have studied the effects of GM-CSF on cell growth in Dexter's type normal human long-term bone marrow cultures. Non adherent and adherent cells were weekly harvested and studied all over the time of culture up to 10 weeks: number, cytology, hematopoietic granulocyte-macrophage progenitors content (CFU-GM). Control consisted of cultures without growth factor. GM-CSF (100 ng/ml) induced a significant increase in the number of non adherent and adherent cells, mainly increasing the number of cells of the granulocytic lineage. GM-CSF also induced a transient increase in the number of CFU-GM in the non adherent fraction, but as from week 6 of culture, there were no more CFU-GM. At last GM-CSF inhibited completely or partially the adipocyte growth in the adherent stromal cell layers. In conclusion, at this concentration, GM-CSF might be responsible for the accelerated aging, maybe linked to the hematopoietic stem cells exhaustion and to the almost exclusive presence of monocyte-macrophage cell types in the culture supernatant.
Bonnier
S
S
Laboratoire de Cryobiologie et de Technologie des Greffes de Moelle, Centre de Transfusion Sanguine, Lyon, France.
Campos
L
L
Froehlich
C
C
Guyotat
D
D
D000704:Analysis of Variance; D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006098:Granulocytes / Q000166:cytology; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008264:Macrophages / Q000166:cytology; D013997:Time Factors
D016178:Granulocyte-Macrophage Colony-Stimulating Factor
Modifications de l'hématopoïèse in vitro induites par l'addition de GM-CSF dans des cultures à long terme de moelle humaine normale.