title: A new method of preparing the basal membrane of renal tubules for patch clamp, using beetle malpighian tubules.
abstract: Access to the basal membrane of beetle Malpighian tubules (Onymacris rugatipennis) was achieved by a new method of stripping tubules of the surrounding connective tissue and basement membrane, in a simple squeezing process. We recorded single channel currents in the cell-attached configuration from basal K+ channels. The peeling method described in this paper allows quick and easy preparation of Malpighian tubules for patch clamp studies on the basal membrane.
authors.affiliation: Department of Zoology, University of Cape Town, Rondebosch, South Africa.
authors.forename: S
authors.initials: S
authors.lastname: Nicolson
authors.forename: L
authors.initials: L
authors.lastname: Isaacson
authors.forename: D
authors.initials: D
authors.lastname: Gerneke
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D001517:Coleoptera / Q000502:physiology*; D004553:Electric Conductivity / Q000502:physiology; D005260:Female; D007684:Kidney Tubules / Q000502:physiology* / Q000648:ultrastructure; D008317:Malpighian Tubules / Q000502:physiology* / Q000648:ultrastructure; D008564:Membrane Potentials / Q000502:physiology; D008722:Methods; D008855:Microscopy, Electron, Scanning; D015221:Potassium Channels / Q000502:physiology / Q000648:ultrastructure
chemical_list: D015221:Potassium Channels