Hair cell damage and recovery following chronic application of kanamycin in the chick cochlea.
Three-day old chicks were given kanamycin at a dose of 200 mg/kg/day for 10 days and their cochleae were processed for scanning electron microscopy at 1, 3, 7 and 14 days following the last injection. Both hair cells and supporting cells were damaged by kanamycin in the basal 35% of the basilar papilla. By 14 days post-treatment, however, most of the damaged region had been replaced with regenerating hair cells and supporting cells. The base-to-apex gradient of morphological development along the cochlea was observed in the process of regeneration. Kinocilium and microvilli were observed on the apical surfaces of the regenerating hair cells.
Hashino
E
E
Department of Communication, Tokyo Woman's Christian University, Japan.
Tanaka
Y
Y
Sokabe
M
M
D000818:Animals; D002645:Chickens; D002923:Cilia / Q000187:drug effects / Q000648:ultrastructure; D003051:Cochlea / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D006198:Hair Cells, Auditory / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D007612:Kanamycin / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D008855:Microscopy, Electron, Scanning; D012038:Regeneration; D013997:Time Factors
D007612:Kanamycin