Multiple luteinizing hormone/chorionic gonadotropin receptor messenger ribonucleic acid transcripts.
It has previously been shown that multiple messenger RNA (mRNA) species can be identified in gonadal tissues by probes specific for the LH/CG receptor. Here we show that the sizes and relative abundancies of gonadal LH/CG receptor transcripts are quite variable between such closely related species as rat and mouse. These patterns of LH/CG receptor mRNAs are yet different from that observed in human embryonic kidney 293 cells that have been transfected with a cDNA encoding for the rat luteal LH/CG receptor. In spite of the diversity in the number and sizes of LH/CG receptor mRNA transcripts, however, our data also show that the size of the cell surface receptor expressed in these three cells/tissues is identical. We further show that the most abundant LH/CG receptor mRNA present in MA-10 cells, a clonal strain of cultured Leydig tumor cells, is a 1.2 kilobase transcript which encodes for a truncated version of the LH/CG receptor corresponding to the extracellular hormone-binding domain. It does not appear, however, that this transcript is translated into a functional protein.
Wang
H
H
Department of Physiology and Biophysics, University of Iowa, Iowa City 52242.
Ascoli
M
M
Segaloff
D L
DL
D000818:Animals; D006063:Chorionic Gonadotropin / Q000378:metabolism; D003338:Corpus Luteum / Q000378:metabolism; D004247:DNA / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D007668:Kidney / Q000196:embryology / Q000378:metabolism; D007984:Leydig Cell Tumor; D008297:Male; D009693:Nucleic Acid Hybridization; D010053:Ovary / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011974:Receptors, LH / Q000235:genetics*; D012708:Sertoli Cells / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured
D006063:Chorionic Gonadotropin; D012333:RNA, Messenger; D011974:Receptors, LH; D004247:DNA