A serological survey on Bacillus piliformis infection in laboratory rabbits in Japan.
A total of 544 rabbit sera obtained from 6 commercial breeding facilities and 9 research institutions during 1985-1990 were tested for Bacillus piliformis antibody by an enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescent antibody test (IFAT). The antibody was detected in 53 (14.2%) rabbits from 3 breeding facilities and 30 (17.4%) rabbits from 6 research institutions, indicating the prevalence of B. piliformis infection among laboratory rabbits in Japan. The overall agreement with ELISA for immune status was 96.9% (527/544) with IFAT. In tests of the ability of ELISA and IFAT to quantitate antibody, a correlation coefficient (r) of 0.86 (P less than 0.01) was obtained by plotting the measured average log of the ELISA titer against the corresponding log of the IFAT titer.
Goto
K
K
Central Institute for Experimental Animals, Kanagawa, Japan.
Itoh
T
T
Takakura
A
A
Kunita
S
S
Terada
E
E
Kagiyama
N
N
D000818:Animals; D000830:Animals, Laboratory / Q000276:immunology; D000907:Antibodies, Bacterial / Q000032:analysis; D001407:Bacillus / Q000276:immunology; D001424:Bacterial Infections / Q000453:epidemiology / Q000276:immunology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D007564:Japan / Q000453:epidemiology; D015995:Prevalence; D011817:Rabbits / Q000276:immunology
D000907:Antibodies, Bacterial