Human hematopoietic stem cells in long-term culture: quantitation and manipulation.
These results suggest a potentially important role for the LTC system, not only in the development of a quantitative assay for hematopoietic cells, but also clinically as a procedure for manipulating autologous marrow in culture in a variety of ways for therapeutic advantage. These include strategies for immune modulation, gene marking and gene therapy, and perhaps eventually for stem cell amplification.
Barnett
M J
MJ
Leukemia/Bone Marrow Transplantation Program of British Columbia, Vancouver General Hospital.
Sutherland
H J
HJ
Eaves
A C
AC
Hogge
D E
DE
Humphries
R K
RK
Klingemann
H G
HG
Lansdorp
P M
PM
Phillips
G L
GL
Reece
D E
DE
Shepherd
J D
JD
D016026:Bone Marrow Transplantation; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans