Increases in transforming growth factor-beta mRNA in hippocampus during response to entorhinal cortex lesions in intact and adrenalectomized rats.
Transforming growth factor-beta mRNA was detected with a rat TGF-beta 1 coding sequence probe as a 2.5 kb band by RNA blot hybridization of total RNA from the adult male rat hippocampus. Following electrolytic lesions of the entorhinal cortex that cause hippocampal deafferentation and synaptic remodeling, TGF-beta mRNA increases 5-fold in ipsilateral hippocampus when compared with intact controls. This increase was independent of prior adrenalectomy or corticosterone-replacement. These data demonstrate that TGF-beta gene expression increases in response to hippocampal deafferentation.
Nichols
N R
NR
Ethel Percy Andrus Gerontology Center, Los Angeles, CA.
Laping
N J
NJ
Day
J R
JR
Finch
C E
CE
D000302:Adrenal Cortex / Q000502:physiology*; D000315:Adrenalectomy; D000344:Afferent Pathways / Q000502:physiology; D000818:Animals; D003345:Corticosterone / Q000494:pharmacology / Q000502:physiology*; D003714:Denervation; D006624:Hippocampus / Q000378:metabolism*; D008032:Limbic System / Q000502:physiology*; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011916:Rats, Inbred F344; D016212:Transforming Growth Factor beta / Q000096:biosynthesis*
D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D003345:Corticosterone