Comparison of the effects of neuropeptide Y and noradrenaline on rat gastric mucosal blood flow and integrity.
1. The effects of neuropeptide Y (NPY) and noradrenaline on rat gastric mucosal blood flow, as estimated by laser Doppler flowmetry (LDF), have been examined. In addition, the ability of NPY and noradrenaline to induce acute mucosal haemorrhagic damage has also been assessed. 2. Close-arterial infusion of NPY (0.05-0.2 nmol kg-1 min-1) for 10 min in the anaesthetized rat induced a dose-dependent fall in LDF, but had minimal effects on systemic arterial blood pressure. Higher doses of NPY did not produce any further reduction in LDF. 3. Close-arterial infusion (0.1-0.4 nmol kg-1 min-1) of the structurally related peptide YY (PYY) or pancreatic polypeptide (PP), had inconsistent actions in decreasing LDF. 4. Close-arterial infusion of noradrenaline (30-90 nmol kg-1 min-1) dose-dependently reduced gastric LDF. 5. Local infusion of NPY (0.1 and 0.2 nmol kg-1 min-1) or noradrenaline (45 and 60 nmol kg-1 min-1) resulted in dose-related increases in the area of mucosal hemorrhagic damage. 6. Pretreatment with the alpha 1-adrenoceptor antagonist, prazosin (0.1 mg kg-1, i.v.) significantly reduced the effect of noradrenaline, but not NPY, on both LDF and mucosal damage. 7. These findings indicate that NPY and noradrenaline act directly on the gastric microvasculature to induce vasoconstriction and both can induce acute mucosal damage. Therefore endogenous NPY, like noradrenaline could play a modulatory role in regulating vascular tone and may influence mucosal integrity.
Tepperman
B L
BL
Department of Pharmacology, Wellcome Research Laboratories, Beckenham, Kent.
Whittle
B J
BJ
D000758:Anesthesia; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005753:Gastric Mucosa / Q000098:blood supply* / Q000187:drug effects; D006471:Gastrointestinal Hemorrhage / Q000139:chemically induced / Q000473:pathology; D066298:In Vitro Techniques; D008297:Male; D009478:Neuropeptide Y / Q000494:pharmacology* / Q000633:toxicity; D009638:Norepinephrine / Q000494:pharmacology* / Q000633:toxicity; D010191:Pancreatic Polypeptide / Q000494:pharmacology; D011224:Prazosin / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012039:Regional Blood Flow / Q000187:drug effects; D013276:Stomach Ulcer / Q000139:chemically induced
D009478:Neuropeptide Y; D010191:Pancreatic Polypeptide; D009638:Norepinephrine; D011224:Prazosin