Interleukin-1 mRNA-expressing macrophages in human chronically inflamed gingival tissues.
Interleukin-1 (IL-1)-producing cells in inflamed gingival tissues obtained from patients with periodontitis were examined by combined immunohistochemistry and in situ hybridization. Macrophages, T cells, B cells, and endothelial cells were visualized in the lesions by the immunoperoxidase method using specific antibodies to each cell type. Subsequent in situ hybridization using 35S-labeled synthetic oligonucleotide probes complementary to human IL-1 alpha and IL-1 beta mRNA showed IL-1 transcripts in macrophages predominantly but not in T cells or B cells. Neither fibroblasts nor keratinocytes contained the IL-1 message to any significant extent. Endothelial cells also were essentially negative for IL-1 messages. These findings indicate that IL-1 is produced principally by macrophages in inflamed gingival tissues of humans.
Matsuki
Y
Y
Department of Periodontology, Niigata University School of Dentistry, Japan.
Yamamoto
T
T
Hara
K
K
D000328:Adult; D001345:Autoradiography; D002908:Chronic Disease; D005881:Gingiva / Q000378:metabolism* / Q000473:pathology; D005891:Gingivitis / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000235:genetics*; D008264:Macrophages / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*
D007375:Interleukin-1; D012333:RNA, Messenger