Endothelin-1 induces prostacyclin release from bovine aortic endothelial cells.
The effects of endothelin-1 (ET-1) on the release of prostacyclin from cultured bovine aortic endothelial cells were studied. ET-1 induced a time- and dose-dependent release of 6-keto PGF1 alpha, the stable metabolite of prostacyclin, with an apparent EC50 value of 3.0 +/- 0.9 nM (n = 6). ET-1 up to a concentration of 500 nM did not affect cellular integrity. Preincubation of the cells for 30 min with 10 microM indomethacin inhibited ET-1 (100 nM) - induced prostacyclin release by 90%. These findings indicate that ET-1 can directly stimulate prostacyclin release from endothelial cells probably through a receptor mediated mechanism.
Filep
J G
JG
Department of Pharmacology, Faculty of Medicine, University of Sherbrooke, P.Q., Canada.
Battistini
B
B
Côté
Y P
YP
Beaudoin
A R
AR
Sirois
P
P
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism*; D000818:Animals; D001011:Aorta; D002417:Cattle; D002478:Cells, Cultured; D016232:Endothelins / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D007213:Indomethacin / Q000494:pharmacology; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D013917:Thrombin / Q000494:pharmacology
D016232:Endothelins; D015121:6-Ketoprostaglandin F1 alpha; D007770:L-Lactate Dehydrogenase; D013917:Thrombin; D007213:Indomethacin