Encapsulated hepatocytes for controlling hyperbilirubinemia in Gunn rats.
Hepatocytes encapsulated in an alginate-polylysine-membrane were used to lower bilirubin levels in hyperbilirubinemia. The animal model was the Gunn rat. The microencapsulated hepatocytes were implanted intraperitoneally. 15 x 16(6) microencapsulated hepatocytes from Wistar rats lowered the bilirubin from 14 mg/100 ml to 6 mg/100 ml after 20 days. 15 x 10(6) microencapsulated hepatocytes from Sprague-Dawley rats lowered the bilirubin levels from 8 mg/100 ml to 5 mg/100 ml. They were as effective as free Sprague-Dawley hepatocytes. 12 x 10(6) Guinea pig hepatocytes, whether encapsulated or free, lowered the bilirubin from 9.36 mg/100 ml to 4.37 mg/100 ml.
Bruni
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Artificial Cells and Organs Research Centre, Faculty of Medicine, Mc Gill University, Montreal, Quebec, Canada.
Chang
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TM
D000464:Alginates; D000818:Animals; D001663:Bilirubin / Q000097:blood*; D006168:Guinea Pigs; D006932:Hyperbilirubinemia / Q000601:surgery*; D008099:Liver / Q000166:cytology*; D016031:Liver Transplantation; D008567:Membranes, Artificial; D011107:Polylysine; D051381:Rats; D011911:Rats, Gunn; D011919:Rats, Inbred Strains
D000464:Alginates; D008567:Membranes, Artificial; D011107:Polylysine; D001663:Bilirubin