Expression of p53 mutant protein(s) in diethylnitrosamine-induced foci of enzyme-altered hepatocytes in male Fischer-344 rats.
Several types of human and animal tumors have been shown to carry mutations in the p53 gene. While the translation product of the wild type gene has tumor suppressor properties, mutant alleles of the gene produce proteins that can cooperate with other oncogene products in transforming cells. In this paper, evidence is presented indicating that a p53 gene mutation(s) occurs in foci of enzyme-altered hepatocytes induced by diethylnitrosamine in male Fisher-344 rats. The evidence was obtained by means of immunohistochemical and immunoblotting techniques, using antibodies directed against mutant forms of the p53 protein.
Smith
M L
ML
Department of Pathology, School of Medicine, University of Pittsburgh, PA 15261.
Yeleswarapu
L
L
Locker
J
J
Lombardi
B
B
D000818:Animals; D004052:Diethylnitrosamine; D016158:Genes, p53; D005982:Glutathione Transferase / Q000032:analysis; D015151:Immunoblotting; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis; D008114:Liver Neoplasms, Experimental / Q000235:genetics* / Q000378:metabolism; D008297:Male; D009154:Mutation; D011230:Precancerous Conditions / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D016159:Tumor Suppressor Protein p53 / Q000032:analysis* / Q000235:genetics
D007527:Isoenzymes; D016159:Tumor Suppressor Protein p53; D004052:Diethylnitrosamine; D005982:Glutathione Transferase