Cloning and DNA sequence analysis of the serC-aroA operon from Salmonella gallinarum; evolutionary relationships between the prokaryotic and eukaryotic aroA-encoded enzymes.
The serC-aroA operon of Salmonella gallinarum was isolated from a gene library using a labelled oligonucleotide probe and by complementation of an aroA Escherichia coli strain. The nucleotide sequence of a 2.6 kbp fragment was determined. The predicted amino acid sequence of the aroA gene product was compared to the equivalent sequence from ten other organisms. Computer-generated evolutionary trees clearly divide the eleven sequences into four different groups: Gram-negative bacteria, Gram-positive bacteria, fungi and plants. These trees depict a close evolutionary relationship between the sequences from Gram-negative bacteria and higher plants.
Griffin
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Division of Molecular Biology, AFRC Institute for Animal Health, Houghton Laboratory, Huntingdon, Cambridgeshire, UK.
Griffin
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AM
D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D019883:Alkyl and Aryl Transferases; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D005075:Biological Evolution; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry*; D001330:Electronic Data Processing; D004926:Escherichia coli / Q000235:genetics; D005658:Fungi / Q000235:genetics*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009876:Operon; D010944:Plants / Q000235:genetics*; D012475:Salmonella / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D012765:Shikimic Acid / Q000031:analogs & derivatives; D014166:Transferases / Q000235:genetics*
D001426:Bacterial Proteins; C058070:5-enolpyruvoylshikimate-3-phosphate; D012765:Shikimic Acid; D004247:DNA; D014166:Transferases; D019883:Alkyl and Aryl Transferases; D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase