The biosynthesis of proteoglycans and interstitial collagens by bovine pericardial fibroblasts.
The biosynthesis of interstitial collagens (types I and III) and proteoglycans was studied in fibroblasts isolated from the parietal layer of bovine pericardium. Confluent cultures were labeled with Na2 35SO4 for proteoglycans or 14C-proline for collagens. The proteoglycans synthesized by pericardial fibroblasts were purified by DEAE-Sephacel chromatography and further fractionated into three components by gelfilitration. Two minor high molecular weight proteoglycans were shown by SDS-PAGE to be resistant to chondroitinase ABC and AC, and partially degraded by nitrous acid. The major, low molecular weight proteoglycan had a core protein of 45 kDa and is considered to be a dermatan sulfate/chondroitin sulfate proteoglycan since it was resistant to nitrous acid, but digested partially by chondroitinase AC and completely by ABC. The pericardial fibroblasts synthesized predominantly type I collagen and low amounts (about 10%) of type III collagen which was detected by delayed reduction on SDS-PAGE. The data show that pericardial fibroblasts synthesize the same macromolecules that can be extracted from the intact tissue and suggest that the proteoglycan may play a structural as well as physiological role.
Simionescu
D T
DT
Department of Medicine, University of Pennsylvania, Philadelphia 19104.
Kefalides
N A
NA
D000818:Animals; D002417:Cattle; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D005347:Fibroblasts / Q000378:metabolism; D066298:In Vitro Techniques; D008970:Molecular Weight; D009608:Nitrous Acid / Q000737:chemistry; D010449:Peptide Mapping; D010496:Pericardium / Q000378:metabolism*; D011509:Proteoglycans / Q000096:biosynthesis* / Q000737:chemistry
D011509:Proteoglycans; D003094:Collagen; D009608:Nitrous Acid