An assay for complement factor B in species at different levels of evolution.
The alternative complement pathway is regarded as the evolutionary ancestral complement system. It is therefore of interest to analyze and characterize factor B of the alternative complement pathway in animals at different levels of evolution. We describe a functional assay which is based on the interaction of factor B from various animal species with Cobra Venom Factor, the snake C3b-analog. The signal system in the assay is C3-conversion in factor B-depleted chicken serum. The results show that a functional C3-convertase can be created with factor B from mammals, aves, and amphibians.
Jensen
L B
LB
Hybridoma Laboratory, Statens Seruminstitut, Copenhagen S, Denmark.
Koch
C
C
D000818:Animals; D005075:Biological Evolution; D050577:Complement C3-C5 Convertases; D011415:Complement Factor B / Q000032:analysis* / Q000378:metabolism; D003170:Complement Pathway, Alternative; D004546:Elapid Venoms; D006358:Hot Temperature; D006801:Humans; D008274:Magnesium; D008722:Methods
D004546:Elapid Venoms; C016310:cobra venom factor; D050577:Complement C3-C5 Convertases; D011415:Complement Factor B; D008274:Magnesium