title: Glass-bead column separation of motile and nonmotile human spermatozoa.
abstract: Glass-bead columns were tested for their efficiency in concentrating motile human spermatozoa from frozen semen samples. The data show that glass-bead filtration concentrates the motile gametes in each sample and is significantly more efficient than the swim-up method for obtaining populations of motile spermatozoa. The data suggest that this method can be applied clinically to obtain motile spermatozoa from poor-quality semen for use in in vitro fertilization.
authors.affiliation: Department of Biology, Temple University, Philadelphia, Pennsylvania 19122.
authors.forename: N
authors.initials: N
authors.lastname: Lechtzin
authors.forename: W
authors.initials: W
authors.lastname: Garside
authors.forename: S
authors.initials: S
authors.lastname: Heyner
authors.forename: N
authors.initials: N
authors.lastname: Hillman
mesh_terms: D002452:Cell Count; D002469:Cell Separation / Q000379:methods*; D015925:Cryopreservation; D005374:Filtration / Q000379:methods*; D005615:Freezing; D005898:Glass; D006801:Humans; D008297:Male; D013081:Sperm Motility; D013094:Spermatozoa