Measurement of thyroid hormone in the rat thyroid gland by radioimmunoassay.
The present paper describes (i) a hydrolysis technique with Pronase and leucine aminopeptidase using one rat thyroid gland, resulting in maximum release of thyroid hormones and minimum deiodination, and (ii) a simple and rapid procedure for thyroid hormone radioimmunoassays in thyroid hydrolysates using commercial kits intended for serum thyroid hormone determinations. The procedure is used to determine T4, T3, and rT3 concentrations and hormonal molar ratios in a thyroid gland from a male Wistar rat. The reliability of the method is also studied.
Chomard
P
P
Laboratoire de Chimie Analytique, UFR des Sciences Pharmaceutiques et Biologiques, Dijon, France.
Autissier
N
N
D000818:Animals; D002138:Calibration; D005615:Freezing; D006868:Hydrolysis; D007455:Iodine; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008297:Male; D011402:Pronase / Q000378:metabolism; D011863:Radioimmunoassay / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013961:Thyroid Gland / Q000737:chemistry*; D013963:Thyroid Hormones / Q000032:analysis*; D013997:Time Factors
D013963:Thyroid Hormones; D007455:Iodine; D007931:Leucyl Aminopeptidase; D011402:Pronase