A competitive enzyme immunoassay for albuterol: its application for the drug screening in urine.
A competitive enzyme immunoassay using purified monoclonal IgG1 and an alkaline phosphatase-albuterol derivative has been developed for the quantification of albuterol in urine. The calibration curve obtained in optimal incubation conditions is characterized by a minimum detectable level of 26 fmol/well and a working range from 52 fmol to 4,2 pmol/well. This method allows the precise and accurate quantification of albuterol in horse urine without any clean up or extraction step. Moreover the definition of its specificity shows a cross-reactivity of the antibody with the glucurono-/sulfo-conjugates of albuterol. This property is particularly interesting for the screening of urinary albuterol residues in meat producing animals.
Adam
A
A
Faculté de pharmacie, Université de Montréal, Québec, Canada.
Ong
H
H
Gravel
A
A
Messier
J
J
Bellemare
M
M
Lantin
F
F
Sauvé
G
G
Tyssen
P
P
D000420:Albuterol / Q000276:immunology / Q000652:urine*; D000469:Alkaline Phosphatase; D000818:Animals; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D006736:Horses; D008460:Meat / Q000032:analysis; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000469:Alkaline Phosphatase; D000420:Albuterol