title: A putative mouse oocyte maturation inhibitory protein from urine of pregnant women: N-terminal sequence homology with human nonsecretory ribonuclease.
abstract: A putative mouse oocyte maturation inhibitory protein was purified from a urine preparation from pregnant women by Sephadex G-100 gel filtration and reverse-phase chromatography on the basis of inhibitory activity of polar body formation of denuded mouse oocytes in culture. Amino terminal sequence analyses showed that residues 5 to 15 of this protein were identical to residues 1 to 11 of human nonsecretory ribonuclease. Furthermore, residues 1 to 4 of this protein were identical to residues -4 to -1, corresponding to part of a signal peptide region of eosinophil-derived neurotoxin, whose mature sequence is identical to nonsecretory ribonuclease. These results indicate that the protein purified as a putative mouse oocyte maturation inhibitory protein from the urine of pregnant women may be a product of an peculiar processing of a nonsecretory ribonuclease precursor.
authors.affiliation: Department of Biochemistry, School of Pharmaceutical Sciences, Nagasaki University, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Sakakibara
authors.forename: K
authors.initials: K
authors.lastname: Hashida
authors.forename: N
authors.initials: N
authors.lastname: Tominaga
authors.forename: K
authors.initials: K
authors.lastname: Sakai
authors.forename: M
authors.initials: M
authors.lastname: Ishiguro
authors.forename: S
authors.initials: S
authors.lastname: Imamura
authors.forename: F
authors.initials: F
authors.lastname: Ohmatsu
authors.forename: E
authors.initials: E
authors.lastname: Sato
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009865:Oocytes / Q000187:drug effects*; D010455:Peptides / Q000235:genetics / Q000652:urine*; D011247:Pregnancy / Q000652:urine*; D012260:Ribonucleases / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C018355:intraovarian peptides; D012260:Ribonucleases