Polyacrylic microspheres as a solid phase for microparticle enhanced nephelometric immunoassay (NEPHELIA (R)) of transferrin.
Polyfunctional hydrophilic microspheres (MS) can be produced by copolymerisation with gamma-irradiation of acrylic monomers. Transferrin (TRF) can be covalently bound to these MS by reaction between aldehyde groups of the MS and primary amino groups of the protein. MS-TRF conjugates thus obtained are agglutinated by specific antiserum and this agglutination is inhibited by free TRF. Agglutination and inhibition are quantified by measurement of the light scattered by MS-TRF conjugate clusters with a specially designed nephelometer, a process designated as microparticle enhanced nephelometric immunoassay (NEPHELIA (R)) for TRF. Recovery, correlation and reproducibility studies, simultaneously performed in three different laboratories, show that this TRF immunoassay is accurate for a large concentration range. NEPHELIA (R) may appear as an alternative method for a large variety of molecules.
Montagne
P
P
Immunology Laboratory, Faculty of Medicine, Nancy, France.
Laroche
P
P
Cuilliere
M L
ML
Riochet
D
D
Flecheux
O
O
Varcin
P
P
Marchand
J
J
Pau
B
B
Duheille
J
J
D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008863:Microspheres; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D012015:Reference Standards; D014168:Transferrin / Q000032:analysis* / Q000592:standards
D014168:Transferrin