Radioimmunoassay of epidermal growth factor in human saliva and gastric juice.
A sensitive radioimmunoassay was developed for human epidermal growth factor (hEGF) in saliva and gastric juice. This method was sufficiently sensitive for an accurate measurement of hEGF in these biological fluids. The minimal detectable concentration of EGF was 30 ng/L. The imprecision profile of EGF standard curve had a CV less than 10% in the range of 0.1-3.0 micrograms/L. Serial dilution curves of saliva and gastric juice paralleled that of standard EGF. The antibody to hEGF showed no cross-reactivity with a large excess of growth factors, such as human transforming growth factor alpha, human insulin-like growth factor I, and platelet-derived growth factor (c-sis). No detectable cross-reactivity was observed with some biological gut peptides: somatostatin, gastrin, secretin or pancreatic polypeptide. The intra-assay CV for saliva and gastric juice was less than 10%, and the recoveries were 93.9 +/- 8.7% and 93.7 +/- 11.3%, respectively for saliva and gastric juice. Gel exclusion chromatography revealed hEGF-like substances, heterogeneous in size in saliva and gastric juice, the origins and physiological functions of which are unknown.
Orsini
B
B
Department of Clinical Pathophysiology, University of Florence, Italy.
Brocchi
A
A
Calabrò
A
A
Fedi
P
P
Tommasi
M S
MS
Surrenti
C
C
D000328:Adult; D000368:Aged; D000906:Antibodies; D001667:Binding, Competitive; D002850:Chromatography, Gel; D003429:Cross Reactions; D004815:Epidermal Growth Factor / Q000032:analysis* / Q000276:immunology; D005260:Female; D005750:Gastric Juice / Q000737:chemistry*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay / Q000379:methods*; D012463:Saliva / Q000737:chemistry*
D000906:Antibodies; D004815:Epidermal Growth Factor