title: Crystallization of the calcium-activated phosphoenolpyruvate carboxykinase from Escherichia coli K12.
abstract: Single crystals of phosphoenolpyruvate carboxykinase from Escherichia coli K12 have been grown in the orthorhombic crystal system. Single crystals developed to a maximum size of 0.25 mm x 0.25 mm x 1.5 mm by the technique of washing and reseeding. The space group is P2(1)2(1)2(1), with a = 77.24 A, b = 89.18 A, c = 93.24 A and Z = 4; there is one enzyme molecule per crystallographic asymmetric unit and the solvent content is estimated to be 59%. The crystals diffract to at least 2.8 A d spacings and decompose in the X-ray beam after approximately two days of exposure.
authors.affiliation: Department of Biochemistry, University of Saskatchewan, Saskatoon, Canada.
authors.forename: L T
authors.initials: LT
authors.lastname: Delbaere
authors.forename: M
authors.initials: M
authors.lastname: Vandonselaar
authors.forename: D
authors.initials: D
authors.lastname: Glaeske
authors.forename: C
authors.initials: C
authors.lastname: Jabs
authors.forename: H
authors.initials: H
authors.lastname: Goldie
mesh_terms: D002118:Calcium / Q000378:metabolism*; D003460:Crystallization; D004926:Escherichia coli / Q000201:enzymology*; D015394:Molecular Structure; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000737:chemistry* / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D010729:Phosphoenolpyruvate Carboxykinase (GTP); D002118:Calcium