title: Multi-parameter flow cytometric quantitation of the expression of the tumor-associated antigen SM3 in normal and neoplastic ovarian tissues. A comparison with HMFG1 and HMFG2.
abstract: SM3 is a monoclonal antibody that reacts with a peptide epitope in the core protein of polymorphic epithelial mucin. Multi-parameter flow cytometry was used to characterize the expression of SM3 and compare it with two related tumor-associated antigens, HMFG1 and HMFG2, in cell suspensions of 44 malignant ovarian tumors, 15 benign ovarian tumors, and 16 normal ovaries. Tumor-associated antigen expression was significantly higher in malignant ovarian neoplasms than in benign neoplasms (P less than 0.001 for all three antigens). SM3 was expressed more specifically in malignant than benign tumors but had a lower affinity than HMFG1 and HMFG2. Multi-parameter flow cytometric evaluation of a panel of monoclonal antibodies can be used to help in choosing the best antibody for immunohistochemistry, imaging, and eventually treatment of ovarian tumors.
authors.affiliation: Gynaecological Oncology Unit, Saint Bartholomew's Hospital, West Smithfield, London, UK.
authors.forename: P A
authors.initials: PA
authors.lastname: van Dam
authors.forename: D G
authors.initials: DG
authors.lastname: Lowe
authors.forename: J V
authors.initials: JV
authors.lastname: Watson
authors.forename: T W
authors.initials: TW
authors.lastname: Jobling
authors.forename: T
authors.initials: T
authors.lastname: Chard
authors.forename: J H
authors.initials: JH
authors.lastname: Shepherd
mesh_terms: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis*; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000032:analysis*; D018396:Mucin-1; D010051:Ovarian Neoplasms / Q000235:genetics / Q000276:immunology*; D010053:Ovary / Q000276:immunology*; D011003:Ploidies; D012016:Reference Values; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D004273:DNA, Neoplasm; D008562:Membrane Glycoproteins; D018396:Mucin-1