title: The effect of an N-methyl-D-aspartate lesion in the hippocampus on glial and neuronal marker proteins.
abstract: The study employed an immunochemical quantification of brain cell marker proteins in addition to quantitative morphology in order to provide a more multifacetted and characterized model for an excitotoxic CNS lesion. The importance of the approach in the evaluation of the potential of neuroprotective agents is emphasized. The S-100 protein, the glial fibrillary acidic (GFA) protein, neuron specific enolase (NSE) and neuronal intermediary filament polypeptides (NF 68 and NF 200) were measured with a dot-immunobinding assay, 3-30 days after a unilateral injection of N-methyl-D-aspartate (NMDA) in the left dorsal hippocampus of the rat. After 3 days, the neuronal cell loss averaged 80% in the hippocampus. The S-100 content was reduced 3 days after injection, but was 150% of control at 30 days. GFA increased constantly from days 3 to 30. The neuronal marker proteins were all markedly reduced 7 days after injection. However, at 30 days, NF 68 and NF 200 were close to control (80%). Increasing content would reflect regeneration and sprouting of neurites. The content of the neuronal cytoplasmic marker, NSE, was significantly lower than control also at 10 and 30 days, although a gradual recovery could be traced.
authors.affiliation: Institute of Neurobiology, University of Göteborg, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.forename: G J
authors.initials: GJ
authors.lastname: Lees
authors.forename: L E
authors.initials: LE
authors.lastname: Rosengren
authors.forename: J E
authors.initials: JE
authors.lastname: Karlsson
authors.forename: T
authors.initials: T
authors.lastname: Stigbrand
authors.forename: A
authors.initials: A
authors.lastname: Hamberger
authors.forename: K G
authors.initials: KG
authors.lastname: Haglid
mesh_terms: D000818:Animals; D015415:Biomarkers; D002470:Cell Survival / Q000187:drug effects; D003593:Cytoplasm / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein / Q000276:immunology / Q000378:metabolism; D006624:Hippocampus / Q000033:anatomy & histology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D016202:N-Methylaspartate / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000276:immunology / Q000378:metabolism*; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009929:Organ Size / Q000187:drug effects; D010477:Perfusion; D010751:Phosphopyruvate Hydratase / Q000276:immunology / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D011919:Rats, Inbred Strains; D009418:S100 Proteins / Q000276:immunology / Q000378:metabolism
chemical_list: D015415:Biomarkers; D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; C030564:S-100 calcium-binding protein alpha subunit; D009418:S100 Proteins; D016202:N-Methylaspartate; D010751:Phosphopyruvate Hydratase