title: Complementary DNA sequencing: expressed sequence tags and human genome project.
abstract: Automated partial DNA sequencing was conducted on more than 600 randomly selected human brain complementary DNA (cDNA) clones to generate expressed sequence tags (ESTs). ESTs have applications in the discovery of new human genes, mapping of the human genome, and identification of coding regions in genomic sequences. Of the sequences generated, 337 represent new genes, including 48 with significant similarity to genes from other organisms, such as a yeast RNA polymerase II subunit; Drosophila kinesin, Notch, and Enhancer of split; and a murine tyrosine kinase receptor. Forty-six ESTs were mapped to chromosomes after amplification by the polymerase chain reaction. This fast approach to cDNA characterization will facilitate the tagging of most human genes in a few years at a fraction of the cost of complete genomic sequencing, provide new genetic markers, and serve as a resource in diverse biological research fields.
authors.affiliation: Section of Receptor Biochemistry and Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD.
authors.forename: M D
authors.initials: MD
authors.lastname: Adams
authors.forename: J M
authors.initials: JM
authors.lastname: Kelley
authors.forename: J D
authors.initials: JD
authors.lastname: Gocayne
authors.forename: M
authors.initials: M
authors.lastname: Dubnick
authors.forename: M H
authors.initials: MH
authors.lastname: Polymeropoulos
authors.forename: H
authors.initials: H
authors.lastname: Xiao
authors.forename: C R
authors.initials: CR
authors.lastname: Merril
authors.forename: A
authors.initials: A
authors.lastname: Wu
authors.forename: B
authors.initials: B
authors.lastname: Olde
authors.forename: R F
authors.initials: RF
authors.lastname: Moreno
mesh_terms: D000595:Amino Acid Sequence; D001331:Automation; D001483:Base Sequence; D001921:Brain / Q000502:physiology*; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics*; D015870:Gene Expression; D015723:Gene Library; D016045:Human Genome Project; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004247:DNA