title: A competitive enzyme immunoassay for albuterol: its application for the drug screening in urine.
abstract: A competitive enzyme immunoassay using purified monoclonal IgG1 and an alkaline phosphatase-albuterol derivative has been developed for the quantification of albuterol in urine. The calibration curve obtained in optimal incubation conditions is characterized by a minimum detectable level of 26 fmol/well and a working range from 52 fmol to 4,2 pmol/well. This method allows the precise and accurate quantification of albuterol in horse urine without any clean up or extraction step. Moreover the definition of its specificity shows a cross-reactivity of the antibody with the glucurono-/sulfo-conjugates of albuterol. This property is particularly interesting for the screening of urinary albuterol residues in meat producing animals.
authors.affiliation: Faculté de pharmacie, Université de Montréal, Québec, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Adam
authors.forename: H
authors.initials: H
authors.lastname: Ong
authors.forename: A
authors.initials: A
authors.lastname: Gravel
authors.forename: J
authors.initials: J
authors.lastname: Messier
authors.forename: M
authors.initials: M
authors.lastname: Bellemare
authors.forename: F
authors.initials: F
authors.lastname: Lantin
authors.forename: G
authors.initials: G
authors.lastname: Sauvé
authors.forename: P
authors.initials: P
authors.lastname: Tyssen
mesh_terms: D000420:Albuterol / Q000276:immunology / Q000652:urine*; D000469:Alkaline Phosphatase; D000818:Animals; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D006736:Horses; D008460:Meat / Q000032:analysis; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D000469:Alkaline Phosphatase; D000420:Albuterol