title: Protein turnover in exponential and stationary phase Vibrio cells.
abstract: Vibrio strain 14 supports phage alpha 3a growth in standing stationary phase cells but not in shaking (aerated) stationary phase cells. In exponential cells, protein was turned over at 1.8% h-1, and the rate was increased by starvation or inhibition of protein synthesis. In shaking stationary phase cells the rate of protein turnover was low (1.0% h-1) for proteins synthesised during growth but high (20% h-1) for recently synthesised proteins. In contrast recently synthesised proteins in standing stationary phase cells were stable over 60 min and proteins synthesised during growth were turned over at 2.9% h-1. ppGpp and pppGpp were detected in exponential cells, but were not detected in stationary phase cells.
authors.affiliation: Department of Medicine, University of the Witwatersrand Medical School, Parktown, Africa.
authors.forename: N B
authors.initials: NB
authors.lastname: Car
authors.forename: D R
authors.initials: DR
authors.lastname: Woods
mesh_terms: D000332:Aerobiosis / Q000502:physiology; D001426:Bacterial Proteins / Q000378:metabolism*; D002455:Cell Division / Q000502:physiology; D006158:Guanosine Pentaphosphate / Q000378:metabolism*; D006159:Guanosine Tetraphosphate / Q000378:metabolism*; D019811:Hydroxylamine; D006898:Hydroxylamines / Q000494:pharmacology; D007930:Leucine / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D009711:Nucleotides / Q000378:metabolism; D010710:Phosphates / Q000494:pharmacology; D014733:Vibrio / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D006898:Hydroxylamines; D009711:Nucleotides; D010710:Phosphates; D019811:Hydroxylamine; D006159:Guanosine Tetraphosphate; D006158:Guanosine Pentaphosphate; D008715:Methionine; D007930:Leucine