Solubilization and purification of rat tissue phospholipase D.
Rat phospholipase D was successfully solubilized with Triton X-100. Solubilized phospholipase D is clearly separated from phosphatidic acid phosphatase and still dependent on sodium oleate for activity.
Kobayashi
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Kanfer
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D000818:Animals; D001921:Brain / Q000201:enzymology*; D002250:Carbon Radioisotopes; D002852:Chromatography, Ion Exchange / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D003902:Detergents / Q000494:pharmacology*; D007202:Indicators and Reagents; D007700:Kinetics; D008168:Lung / Q000201:enzymology*; D008861:Microsomes / Q000201:enzymology*; D010739:Phospholipase D / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D011865:Radioisotope Dilution Technique; D051381:Rats; D012995:Solubility
D002250:Carbon Radioisotopes; D003902:Detergents; D007202:Indicators and Reagents; D010739:Phospholipase D