A simple isocratic ion-pair high-performance liquid chromatographic determination of 1-beta-D-arabinofuranosylcytosine 5'-triphosphate for intracellular drug-monitoring and in vitro incubation assays.
A sensitive isocratic ion-pair HPLC method using a reversed-phase C18 column and phosphate buffer at pH 6 for the determination of the cytotoxic intracellular anabolite 1-beta-D-arabinofuranosylcytosine-triphosphate (Ara-CTP) of the antineoplastic drug cytarabine in leukaemic cells in vivo is described. Simultaneous determination of the physiological nucleotide deoxycytidine-triphosphate is possible. The recovery from cells is greater than 90%, the limit of detection is 25 ng ml-1 (51 nM l-1) and about 10 pM mg-1 cell protein. Extraction procedure and pitfalls are discussed. The method enables intracellular drug monitoring of Ara-CTP with standard HPLC equipment.
Boos
J
J
Department of Pediatrics, University of Münster, FRG.
D001085:Arabinofuranosylcytosine Triphosphate / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007938:Leukemia / Q000378:metabolism; D013644:Tartrates / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D013644:Tartrates; D001085:Arabinofuranosylcytosine Triphosphate; C029768:tartaric acid