Enzyme immunoassay of the active metabolite (RS-5139) of angiotensin converting enzyme inhibitor (CS-622).
A simple and sensitive enzyme immunoassay (EIA) for determination of the active metabolite (RS-5139) of a new angiotensin converting enzyme inhibitor (CS-622) was developed. The N-succinimmidyl ester of RS-5139 was coupled with bovine serum albumin (BSA) and its conjugate was used as an immunogen. Horse radish peroxidase (HRP; EC 1, 11, 1, 7) was used as a labeled enzyme and 3, 3', 5, 5'-tetramethylbenzidine was used as a substrate. The antiserum was used at a final dilution of 1:10000 and sensitivity was 10 pg in plasma and 20pg in urine. CS-622 exhibited cross-reactivity (22.3%), but other ACE inhibitors didn't exhibit cross-reactivity. The plasma levels determined by EIA and GC/MS was good agreement (y = 6.58e-2 + 1.07x, R - 2 = 0.985, n = 52).
Shioya
H
H
Product Development Laboratories, Sankyo Co., Ltd., Tokyo, Japan.
Shimojo
M
M
Kawahara
Y
Y
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D000918:Antibody Specificity; D003429:Cross Reactions; D005069:Evaluation Studies as Topic; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007124:Immunoenzyme Techniques; D011355:Prodrugs / Q000032:analysis / Q000378:metabolism; D013841:Thiazepines / Q000032:analysis* / Q000276:immunology / Q000378:metabolism
D000806:Angiotensin-Converting Enzyme Inhibitors; D011355:Prodrugs; D013841:Thiazepines; C069156:temocaprilat; C055603:temocapril hydrochloride