Reduction of rattlesnake-venom-induced myonecrosis in mice by hyperbaric oxygen therapy.
Hyperbaric oxygen therapy (HBOT) at 1, 2, and 2.75 atmospheres absolute (ATA) was used to treat rattlesnake (Crotalus atrox) venom-induced tissue damage and edema in thigh muscles of mice. Tissue damage was evaluated by double-blind histopathologic examination: tissue edema was determined by measuring tissue water content. A total of 10 intermittent exposures to oxygen over a period of 4 days at 2 and 2.75 ATA did not influence the resolution of venom-induced tissue edema, whereas tissue damage was significantly ameliorated as compared to air-treated envenomated controls. HBOT also promoted healing in the venom-injected mice as evidenced by the presence of regenerating muscle cells. It is concluded that HBOT may limit rattlesnake venom-induced myonecrosis and promote healing in a dose-response relationship without reducing venom-induced edema.
Kelly
J J
JJ
Department of Emergency Medicine, Louisiana State University Medical Center, New Orleans.
Sadeghani
K
K
Gottlieb
S F
SF
Ownby
C L
CL
Van Meter
K W
KW
Torbati
D
D
D000818:Animals; D003435:Crotalid Venoms / Q000009:adverse effects*; D004195:Disease Models, Animal; D004487:Edema / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D005069:Evaluation Studies as Topic; D005260:Female; D006931:Hyperbaric Oxygenation / Q000379:methods / Q000592:standards*; D051379:Mice; D009135:Muscular Diseases / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D009336:Necrosis; D014945:Wound Healing
D003435:Crotalid Venoms