Application of the polymerase chain reaction (PCR) to detection of the aerolysin gene in whole cell cultures of beta-hemolytic Aeromonas hydrophila.
Oligonucleotide primers were used in a polymerase chain reaction protocol to detect the aerolysin gene in Aeromonas hydrophila. Primers detected template DNA in hemolytic, cytotoxic and enterotoxic strains of A. hydrophila and no amplification was detected with hemolytic A. sobria, non-hemolytic A. hydrophila and A. caviae strains.
Lior
H
H
National Laboratory for Enteric Pathogens, Laboratory Centre for Disease Control, Ottawa, Ontario, Canada.
Johnson
W M
WM
D000333:Aeromonas / Q000235:genetics*; D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics*; D002460:Cell Line; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000235:genetics*; D006801:Humans; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods*; D052899:Pore Forming Cytotoxic Proteins
D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D015345:Oligonucleotide Probes; D052899:Pore Forming Cytotoxic Proteins; C032676:aerolysin