The 41 carboxy-terminal residues of the miniF plasmid CcdA protein are sufficient to antagonize the killer activity of the CcdB protein.
The ccd operon of plasmid F encodes two genes, ccdA and ccdB, which contribute to the high stability of the plasmid by post-segregational killing of plasmid-free bacteria. The CcdB protein is lethal to bacteria and the CcdA protein is an antagonist of this lethal action. A 520 bp fragment containing the terminal part of the ccdA gene and the entire ccdB gene of plasmid F was cloned downstream of the tac promoter. Although the CcdB protein was expressed from this fragment, no killing of host bacteria was observed. We found that the absence of killing was due to the presence of a small polypeptide, CcdA41, composed of the 41 C-terminal residues of the CcdA protein. This polypeptide has retained the ability to regulate negatively the lethal activity of the CcdB protein.
Bernard
P
P
Département de Biologie Moléculaire, Université libre de Bruxelles, Rhode Saint Genèse, Belgium.
Couturier
M
M
D001426:Bacterial Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics*; D001427:Bacterial Toxins / Q000037:antagonists & inhibitors* / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D003603:Cytotoxins / Q000037:antagonists & inhibitors* / Q000235:genetics; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D005144:F Factor; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009876:Operon; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000633:toxicity*; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D001427:Bacterial Toxins; C068355:CcdA protein, Bacteria; C068356:CcdB protein, Plasmid F; D003603:Cytotoxins; D004269:DNA, Bacterial; D011993:Recombinant Fusion Proteins