Coincident sequence cloning.
We describe a novel method, Coincident Sequence Cloning (CSC), which permits the selective recovery of common sequences shared between two complex and partially coincident DNA mixtures. We evaluate this method by integrating human DNA with DNA from a mouse-human somatic cell hybrid, and we recover exclusively human DNA products which are all represented in the hybrid genome. CSC strategies should be useful in addressing many highly complex problems in genome analysis.
Brookes
A J
AJ
MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK.
Porteous
D J
DJ
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D004247:DNA