Is the oxidation of milacemide by monoamine oxidase a major factor in its anticonvulsant actions?
The anticonvulsant drug milacemide (2-n-pentylaminoacetamide) is known to be oxidized by monoamine oxidase-B to yield glycinamide which then breaks-down to give glycine. It has been postulated that it is this liberation of glycine in the brain that accounts for the anticonvulsant effects. In order to test this hypothesis, and since amines bearing a methyl-group in the alpha-position have been shown to be resistant to oxidation by monoamine oxidase, the effects of milacemide were compared with those of alpha-methyl-milacemide. Although the latter compound was found to be toxic at higher concentrations, it was found to antagonize bicuculline-induced convulsions in mice. When milacemide was administered to mice (0.5 mmol/kg, p.o.) there was a substantial increase in urinary glycinamide excretion. No such increase was observed after the administration of the same dose of alpha-methyl-milacemide. Furthermore, alpha-methyl-milacemide was not oxidized by either monoamine oxidase-A or -B in vitro to any detectable extent, although it was a competitive inhibitor of both forms of the enzyme. The findings that alpha-methyl-milacemide has anticonvulsant properties in the bicuculline test but is not a substrate for monoamine oxidase or a source of urinary glycinamide cast doubt on the importance of the oxidation or milacemide to form glycinamide as a major factor in its anticonvulsant action.
O'Brien
E M
EM
Department of Biochemistry, Trinity College, Dublin, Ireland.
Tipton
K F
KF
Strolin Benedetti
M
M
Bonsignori
A
A
Marrari
P
P
Dostert
P
P
D000081:Acetamides / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D000927:Anticonvulsants / Q000378:metabolism*; D001640:Bicuculline; D004305:Dose-Response Relationship, Drug; D005998:Glycine / Q000031:analogs & derivatives / Q000378:metabolism; D051379:Mice; D008995:Monoamine Oxidase / Q000378:metabolism*; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D010084:Oxidation-Reduction
D000081:Acetamides; D000927:Anticonvulsants; D008996:Monoamine Oxidase Inhibitors; C039332:milacemide; C069175:alpha-methylmilacemide; C018556:glycine amide; D008995:Monoamine Oxidase; D005998:Glycine; D001640:Bicuculline