title: [Activity of alcohol dehydrogenase from cell cytoplasm of several types of yeast].
abstract: The author studied the activity of alcohol dehydrogenase from the cytoplasm of Saccharomyces cerevisiae, Saccharomyces carlsbergensis, Candida utilis and Torulopsis candida cells. The "classical" alcohol dehydrogenase and the octanol dehydrogenase from the T. candida cytoplasm are active in a wide range of pH from 7.0 to 10.5, whereas in the cytoplasm of other yeast cells these enzymes are active in a relatively narrow range of pH. Essential differences were also revealed in the co-enzyme used. In the cytoplasm of S. carlsbergensis and T. candida cells the enzymes display the activity only in the presence of NAD+, while in the other cells, in the presence of both NAD+ and NADP+. The substrate specificity varies as well. While the general activity of alcohol dehydrogenases from the T. candida cytoplasm decreases gradually with the growth of the alcohol radical from C2 to C10, a different regularity is observed in the other cells. Optimal conditions were chosen for maintaining the enzyme activity during prolonged storage of cytoplasm preparations from the yeasts under study.
authors.forename: V E
authors.initials: VE
authors.lastname: Sudovtsov
mesh_terms: D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D002175:Candida / Q000201:enzymology*; D003593:Cytoplasm / Q000201:enzymology; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D004126:Dimethylformamide / Q000737:chemistry; D005990:Glycerol / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009243:NAD / Q000737:chemistry; D009249:NADP / Q000737:chemistry; D012440:Saccharomyces / Q000201:enzymology*; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D009243:NAD; D012710:Serum Albumin, Bovine; D009249:NADP; D004126:Dimethylformamide; D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase; C021902:octanol dehydrogenase; D005990:Glycerol; D004121:Dimethyl Sulfoxide
vernacular_title: Issledovanie aktivnosti alkogol'degidrogenaz iz tsitoplazmy kletok nekotorykh drozhzheĭ.