title: Use of collagen film as a dural substitute: preliminary animal studies.
abstract: Cadaver grafts, laminated metallic materials, and synthetic fabrics have been evaluated as dural substitutes. Use of cadaver tissues is limited by fear of transmission of infectious disease while use of synthetic materials is associated with implant encapsulation and foreign body reactions. The purpose of this study is to evaluate the use of collagen film as a dural substitute. Collagen films prepared from bovine skin were used to replace the dura of rabbits and histological observations were made at 16, 28, 42, and 56 days postimplantation. Controls consisted of dura that was removed and then reattached. Control dura showed no signs of inflammation or adhesion to underlying tissue at 16 and 28 days postimplantation. By 56 days postimplantation, extensive connective tissue deposition was observed in close proximity to adjacent bone as well as pia arachnoid adhesions. Implanted collagen film behaved in a similar manner to control dura showing minimal inflammatory response at all time periods. At 56 days postimplantation collagen film appeared strongly infiltrated by connective tissue cells that deposited new collagen. The results of this study suggest that a reconstituted type I collagen film crosslinked with cyanamide acts as a temporary barrier preventing loss of fluid and adhesion formation. It is replaced after approximately 2 months with host collagen with limited inflammatory and fibrotic complications. Further studies are needed to completely characterize the new connective tissue formed as well as long-term biocompatibility and functioning of a reconstituted collagen dural substitute.
authors.affiliation: Department of Surgery, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854.
authors.forename: R L
authors.initials: RL
authors.lastname: Collins
authors.forename: D
authors.initials: D
authors.lastname: Christiansen
authors.forename: G A
authors.initials: GA
authors.lastname: Zazanis
authors.forename: F H
authors.initials: FH
authors.lastname: Silver
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001921:Brain / Q000166:cytology; D002417:Cattle; D002448:Cell Adhesion; D003094:Collagen / Q000302:isolation & purification / Q000648:ultrastructure; D004388:Dura Mater / Q000166:cytology / Q000473:pathology / Q000601:surgery*; D011817:Rabbits
chemical_list: D001672:Biocompatible Materials; D003094:Collagen