Eliminating primers from completed polymerase chain reactions with exonuclease VII.
Single-stranded oligonucleotide primers can be efficiently removed after PCR using E.coli exonuclease VII. Even only a few molecules of double stranded PCR product are unaffected by a treatment which eliminates 20 picomoles of primer in the presence of 500 ng of denatured genomic DNA. Exonuclease VII treatment is rapid and could simplify complicated multistep PCR protocols.
Li
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University of Southern California, Los Angeles 90089-1340.
Cui
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Arnheim
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D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000201:enzymology; D005090:Exodeoxyribonucleases / Q000737:chemistry*; D009841:Oligonucleotides; D016133:Polymerase Chain Reaction
D009841:Oligonucleotides; D005090:Exodeoxyribonucleases; C026870:exodeoxyribonuclease VII