Cis-regulatory elements that confer differential expression upon the rat gene encoding phosphoenolpyruvate carboxykinase in kidney and liver.
The PCK gene, encoding cytosolic phosphoenolpyruvate carboxykinase, is specifically expressed in gluconeogenic tissues, liver and kidney. Hence it serves as a model of a class of single-copy genes whose transcription is restricted to a few tissues, rather than a unique tissue. To begin delineating the mechanisms that govern this pattern of expression, cis-regulatory elements of PCK were examined using transient transfection assays in PCK-expressing kidney and hepatoma cell lines. The analyses enabled us to identify a proximal element, between nucleotide (nt) positions -121 and -98, relative to the transcription start point that is sufficient for specific expression in kidney cells, but is just one of the elements required for expression in hepatoma cells. A distal element (between nt -487 and -417), which is essential for hepatoma-specific expression, is not needed in kidney cells. We suggest that the differential regulation of PCK expression in the liver and kidney results from an interplay between different cis-regulatory elements and trans-acting factors.
Shoshani
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Department of Developmental Biochemistry, Hebrew University Hadassah Medical School, Jerusalem, Israel.
Benvenisty
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Trus
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Reshef
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D000818:Animals; D022762:CCAAT-Enhancer-Binding Proteins; D002460:Cell Line; D002872:Chromosome Deletion; D003600:Cytosol; D004268:DNA-Binding Proteins / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008114:Liver Neoplasms, Experimental; D009154:Mutation / Q000502:physiology; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D010730:Phosphoenolpyruvate Carboxylase / Q000235:genetics*; D011401:Promoter Regions, Genetic; D051381:Rats; D013552:Swine; D015534:Trans-Activators / Q000502:physiology; D014157:Transcription Factors / Q000502:physiology*; D014162:Transfection; D014407:Tumor Cells, Cultured; D051840:Y-Box-Binding Protein 1
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human; D010730:Phosphoenolpyruvate Carboxylase