Neighboring nucleotide interactions during DNA sequencing gel electrophoresis.
Electrophoretic separation of oligonucleotides in denaturing polyacrylamide gels is primarily a function of length-dependent mobility. The 3' terminal nucleotide sequence of the oligonucleotide is a significant, secondary determinant of mobility and separation. Oligomers with 3'-ddT migrate more slowly than expected on the basis of length alone, and thus are better separated from the preceding, shorter oligomers in the sequencing ladder. Oligomers with 3'-ddC are relatively faster than expected, and are therefore less separated. At the 3' penultimate position, -dC- increases and -dT- reduces separation. Purines at the 3' terminal or penultimate positions of oligonucleotides affect separation less than the pyrimidines. These results suggest specific interactions among neighboring nucleotides with important effects on the conformation of oligonucleotides during electrophoresis. These interactions are compared to compression artifacts, which represent more extreme anomalies of length-dependent separation of oligonucleotides. Knowledge of base-specific effects on electrophoretic behavior of DNA oligomers supplements the usual information available for determination of sequences; additionally it provides an avenue to thermodynamic and hydrodynamic investigations of DNA structure.
Bowling
J M
JM
Department of Mechanical Engineering, School of Engineering, Vanderbilt University, Nashville, TN 37232.
Bruner
K L
KL
Cmarik
J L
JL
Tibbetts
C
C
D001345:Autoradiography; D001435:Bacteriophages / Q000378:metabolism; D001483:Base Sequence; D004279:DNA, Viral / Q000737:chemistry*; D003854:Deoxyribonucleotides / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D013050:Spectrometry, Fluorescence
D004279:DNA, Viral; D003854:Deoxyribonucleotides