The effect of deuterium labeling on the genotoxicity of N-nitrosodimethylamine, epichlorohydrin and dimethyl sulfate.
Deuterated and non-deuterated N-nitrosodimethylamine, epichlorohydrin and dimethyl sulfate were evaluated for the ability to induce DNA single-strand breaks in rat hepatocytes as measured by alkaline elution. Non-deuterated nitrosodimethylamine induced twice the amount of DNA-strand breaks as the deuterated form. No evidence of a deuterium isotope effect was seen for the direct-acting alkylating agents epichlorohydrin and dimethyl sulfate.
Sargent
E V
EV
Corporate Safety and Industrial Hygiene, Merck and Co., Inc., Rahway, NJ 07065.
Kraynak
A R
AR
Storer
R D
RD
Bradley
M O
MO
Perry
R M
RM
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage; D003903:Deuterium / Q000737:chemistry*; D004128:Dimethylnitrosamine / Q000633:toxicity*; D004811:Epichlorohydrin / Q000633:toxicity*; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D008297:Male; D009153:Mutagens; D051381:Rats; D011919:Rats, Inbred Strains; D013463:Sulfuric Acid Esters / Q000633:toxicity*
D009153:Mutagens; D013463:Sulfuric Acid Esters; D004811:Epichlorohydrin; D003903:Deuterium; C007482:dimethyl sulfate; D004128:Dimethylnitrosamine