Immunochemical studies on a Brucella ovis specific protein antigen.
A Brucella ovis surface protein antigen (P-II), obtained by gel filtration with Sepharose 4B of a hot saline extract was characterized. The analysis of P-II over gradient sodium dodecylsulfate electrophoresis yielded an 18.5 and a 20 kDa band. In a radioimmunoprecipitation assay using P-II labeled with 125I, the antigen reacted specifically only with sera from rams experimentally infected with a naturally occurring rough strain of B. ovis and did not react with sera from rams experimentally infected with other smooth Brucella strains (B. abortus and B. melitensis).
Suarez
C E
CE
Comision Nacional de Energia Atomica, Buenos Aires, Argentina.
Pacheco
G A
GA
Vigliocco
A M
AM
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology; D001667:Binding, Competitive; D002002:Brucella / Q000276:immunology*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D015531:Radioimmunoprecipitation Assay
D000942:Antigens, Bacterial; D000954:Antigens, Surface