Growth characteristics of retinal capillary endothelial cells compared with pulmonary vein endothelial cells in culture. The effect of pericytes on differentiation of endothelial cells.
Bovine retinal capillary endothelial cells (RCECs) and pulmonary vein endothelial cells (PVECs) were isolated and investigated in plate culture, three-dimensional culture and in co-culture with pericytes. In plate culture, RCECs required growth factor in the medium for growth whereas PVECs did not. Phenotypic modulation (a tendency to become similar morphologically to smooth muscle cells, and to accumulate into thread-like structures) was observed in PVECs but not in RCECs. In three-dimensional culture, RCECs contracted, aggregated and were unable to proliferate. Proliferation was elicited when the gel matrix was adsorbed by fibronectin or upon co-culture with pericytes. In contrast, PVECs not only proliferated but also formed tubular structures. In co-culture with pericytes, PVECs in close contact with, or in near apposition to pericytes formed tubular structures earlier than those without contact in the same dish. These results provide new findings about differences in the growth characteristics of endothelial cells between microvessels and large vessels. In addition, it is considered that pericytes may promote tube formation by endothelial cells in three-dimensional culture.
Miyazato
M
M
Department of Pathology, Ryukyu University School of Medicine, Okinawa, Japan.
Fukuda
M
M
Iwamasa
T
T
D000199:Actins / Q000276:immunology / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000276:immunology; D002196:Capillaries / Q000166:cytology* / Q000378:metabolism; D002417:Cattle; D002450:Cell Communication; D002454:Cell Differentiation; D002455:Cell Division; D002469:Cell Separation; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D005353:Fibronectins / Q000276:immunology / Q000378:metabolism; D007150:Immunohistochemistry; D008833:Microcirculation / Q000378:metabolism; D011667:Pulmonary Veins / Q000166:cytology* / Q000378:metabolism; D012160:Retina / Q000166:cytology*; D014841:von Willebrand Factor / Q000276:immunology / Q000378:metabolism
D000199:Actins; D000906:Antibodies; D005353:Fibronectins; D014841:von Willebrand Factor