Linear order of new and established DNA markers around the fragile site at Xq27.3.
We have used recombinant clones derived from microdissection of the fragile X region to characterize breakpoints around the fragile site at Xq27.3. So far, no microdissection markers derived from Xq28 material have been found, thus allowing a rapid screening for clones surrounding the fragile site by their presence in a somatic cell hybrid containing Xq27.2-Xqter. A total of 43 new DNA markers from Xq27 have been sublocalized within this chromosome band. Of these new DNA markers, 5 lie in an interval defined as containing the fragile X region. The saturation of Xq27 with DNA markers by microdissection demonstrates the power of this technique and provides the resources for generating a complete physical map of the region.
Hirst
M C
MC
Molecular Genetics Group, John Radcliffe Hospital, Headington, Oxford, United Kingdom.
Roche
A
A
Flint
T J
TJ
MacKinnon
R N
RN
Bassett
J H
JH
Nakahori
Y
Y
Watson
J E
JE
Bell
M V
MV
Patterson
M N
MN
Boyd
Y
Y
D015139:Blotting, Southern; D002460:Cell Line; D003001:Cloning, Molecular; D005600:Fragile X Syndrome / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics*; D006801:Humans; D009154:Mutation; D016133:Polymerase Chain Reaction; D014960:X Chromosome
D005819:Genetic Markers