Lead speciation by HPLC-ICP-AES and HPLC-ICP-MS.
Speciation of inorganic lead (Pb2+) and several trialkyllead species (trimethyllead chloride [TML], triethyllead chloride [TEL], and triphenyllead chloride [TPhL]) is investigated using high-performance liquid chromatography (HPLC) with detection by both inductively coupled plasma emission spectroscopy (ICP-AES), and inductively coupled plasma mass spectrometry (ICP-MS). Reversed-phase, ion-pairing, and ion-exchange HPLC modes are studied. Optimal chromatographic conditions for ICP-AES detection include a reversed-phase separation utilizing a step gradient from 10 to 70% methanol. However, the gradient has been found to destabilize the plasma when using ICP-MS detection. An isocratic separation with a 30% methanol mobile phase has been found to be the best compromise between plasma stability and chromatographic resolution. Detection limits using ICP-MS detection are 3 orders of magnitude improved over ICP-AES detection.
al-Rashdan
A
A
Department of Chemistry, University of Cincinnati, Ohio 45221.
Heitkemper
D
D
Caruso
J A
JA
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D007854:Lead / Q000032:analysis*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D000432:Methanol; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods*
D007854:Lead; D000432:Methanol