Nucleotide sequence and analysis of the Vibrio alginolyticus scr repressor-encoding gene (scrR).
The nucleotide sequence of the Vibrio alginolyticus scr repressor-encoding gene (scrR) was determined. The deduced amino acid sequence of the scr repressor was homologous with the gal, lac and cyt repressors of Escherichia coli and contained a helix-turn-helix DNA binding domain. Although the scrR gene encoded a protein which was required for the regulation of the V. alginolyticus sucrose utilization system, a particular deletion in the scrR gene could not be complemented in trans. The lack of complementation was discussed in terms of the possible involvement of a cis regulatory element or interference by the truncated scr repressor.
Blatch
G L
GL
Department of Microbiology, University of Cape Town, Rondebosch, South Africa.
Woods
D R
DR
D000595:Amino Acid Sequence; D001483:Base Sequence; D003062:Codon; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D014176:Protein Biosynthesis; D012097:Repressor Proteins / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014733:Vibrio / Q000235:genetics*
D003062:Codon; D012097:Repressor Proteins