Fast-atom-bombardment mass spectrometry of sulphated oligosaccharides from ovine lutropin.
The positive- and negative-ion f.a.b.-mass spectra and the fragmentation of sulphated oligosaccharides derived from ovine lutropin are described. Negative-ion f.a.b.-m.s. of methylated derivatives offers a sensitive and rapid method for screening glycans for sulphation, for defining the location of sulphated residues, and for sequencing sulphated branches. Positive-ion f.a.b.-m.s. gives complementary data on non-sulphated branches in both complex and hybrid-type sulphated structures.
Dell
A
A
Department of Biochemistry, Imperial College, London, Great Britain.
Morris
H R
HR
Greer
F
F
Redfern
J M
JM
Rogers
M E
ME
Weisshaar
G
G
Hiyama
J
J
Renwick
A G
AG
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D007986:Luteinizing Hormone / Q000737:chemistry*; D008745:Methylation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry; D012756:Sheep; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013431:Sulfates / Q000737:chemistry
D009844:Oligosaccharides; D013431:Sulfates; D007986:Luteinizing Hormone