[Specific detection of urinary sympathomimetic amines for control of anti-doping by gas chromatography-mass spectroscopy].
A specific, sensitive and reliable gas chromatography-mass spectrometry (GC-MS) technique for detection of sympathomimetic amines following urinary extraction is proposed. Amphetamine, phentermine, ephedrine, mephenorex, methylphenidate, benzphetamine, clobenzorex and internal standard (fenfluramine) are extracted from urines at pH 7.0 using elution by chloroform-isopropanol on C18 cartridges. Derivatization followed by GC-MS analysis allows identification of these drugs founded on relative retention times and mass spectra. The quantitation limit for derivatizable drugs was found to be 200 ng/ml and 500 ng/ml for underivatizable drugs.
Franceschini
A
A
Laboratoire de Biochimie, Toxicologie et Analyse des Traces, Hôpital Edouard Herriot, France.
Duthel
J M
JM
Vallon
J J
JJ
D000662:Amphetamines / Q000652:urine; D001589:Benzphetamine / Q000652:urine; D004300:Doping in Sports; D005277:Fenfluramine / Q000031:analogs & derivatives / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D013566:Sympathomimetics / Q000652:urine*
D000662:Amphetamines; D013566:Sympathomimetics; D001589:Benzphetamine; D005277:Fenfluramine; C001584:benfluorex; C000490:clobenzorex; C000450:mefenorex
Détection spécifique par chromatographie gazeuse-spectrométrie de masse des amines sympathomimétiques urinaires dans le cadre des contrôles antidopage.