Antigen receptor-mediated calcium signals in B cells as revealed by confocal fluorescence microscopy.
A confocal fluorescence microscope was used to study the antigen receptor-mediated calcium signals in B cells. Anti-IgD binding to B lymphoma cells (BAL17) increased the intracellular calcium concentration with short lag times. Confocal fluorescence images of the fluo-3-loaded BAL17 cells showed that the intracellular calcium ion concentrations increased non-homogeneously, suggesting that the calcium signals transferred not only to the cytoplasm but also to the nucleus.
Yamada
H
H
Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Mizuguchi
J
J
Nakanishi
M
M
D000906:Antibodies / Q000378:metabolism / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D007072:Immunoglobulin D / Q000276:immunology; D007700:Kinetics; D016393:Lymphoma, B-Cell; D008856:Microscopy, Fluorescence; D011947:Receptors, Antigen, B-Cell / Q000502:physiology*; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
D000906:Antibodies; D007072:Immunoglobulin D; D011947:Receptors, Antigen, B-Cell; D002118:Calcium