Platelet adherence and detachment: a flow study with a series of hydroxyethyl methacrylate-ethyl methacrylate copolymers using video microscopy.
The adhesion and detachment of platelets were studied on glass coatings of a series of copolymers of hydroxyethyl methacrylate (HEMA) and ethyl methacrylate (EMA). Observations of the interactions of mepacrine labelled washed platelets with these surfaces from a flowing (500 s-1 wall shear rate) suspension in Tyrode's solution containing albumin and red cells were made with epifluorescent video microscopy (EVM). Total platelet adhesion, including platelets which adhere on first contact and platelets which attach temporarily before adhesion, and the number of detaching platelets were minimal for the 0 and 20% EMA copolymers, reached a maximum for the 50% EMA copolymer and showed reduced values for the 80% and 100% EMA copolymers. For the 50, 80, and 100% EMA copolymers, the adhesion values expressed, as a percentage of total contacting platelets, were not different. Albumin adsorption to these copolymers shows a continuous increase from the 0% to the 100% EMA copolymer. It is likely that the peak in platelet adhesion at the 50% EMA composition is related to: low protein adsorption on the 0 and 20% EMA copolymers, too little albumin adsorption to block adhesion on the 50% EMA copolymer, and full-scale blocking on the 80 and 100% EMA copolymers due to greater albumin adsorption.
Feuerstein
I A
IA
Department of Chemical Engineering, McMaster University, Hamilton, Ontario, Canada.
Buchan
S M
SM
Horbett
T A
TA
Hauch
K D
KD
D000328:Adult; D001672:Biocompatible Materials; D001792:Blood Platelets / Q000502:physiology*; D004912:Erythrocytes / Q000502:physiology; D006801:Humans; D008689:Methacrylates; D008768:Methylmethacrylates; D010973:Platelet Adhesiveness; D013329:Structure-Activity Relationship
D001672:Biocompatible Materials; D008689:Methacrylates; D008768:Methylmethacrylates; C005044:hydroxyethyl methacrylate; C009931:poly(ethylmethacrylate)