Glutathione levels during thermal induction of the yeast-to-mycelial transition in Candida albicans.
Glutathione (GSH) levels were directly monitored by reverse phase HPLC during the thermal yeast-to-mycelial induction of Candida albicans. The GSH levels decreased approximately 100-fold within 120 min which corresponded to the time of maximal yeast-to-mold conversion. The yeast to mold conversion was inhibited by 1-p-chlorophenyl-4,4-dimethyl-5-diethylamino-1-penten-3-one (CDDP), a thiol-specific alkylator, which prevented the decline in GSH levels. These results are discussed with respect to the potential involvement of intracellular GSH levels in regulation of the yeast-to-mold dimorphism in Candida albicans.
Thomas
D
D
Department of Biological Sciences, University of Windsor, Ontario, Canada.
Klein
K
K
Manavathu
E
E
Dimmock
J R
JR
Mutus
B
B
D002176:Candida albicans / Q000502:physiology*; D005978:Glutathione / Q000378:metabolism*; D007700:Kinetics; D013696:Temperature; D013997:Time Factors
D005978:Glutathione