[Chemosensitivity testing by regrowth assay against malignant bone and soft tissue tumors].
In order to improve the clinical results of chemotherapy protocols for patients with malignant bone and soft tissue tumors, an attempt was made to establish a methodological approach to chemo-sensitivity testing using a modified regrowth assay system. We have established a chemo-sensitivity testing system using methylcholanthrene-induced round cell sarcoma in BALB/c mouse. This system was applied clinically and the sensitivity to anticancer drugs was found within two weeks of cell culture. Fifteen patients with malignant tumors were treated using a chemotherapy protocol composed of drugs selected by means of our system. Eleven patients (73.3%) showed an excellent therapeutic response. Our simple assay system is available to determine sensitivity to drugs in a short time. Drugs with no sensitivity can be eliminated from the protocol so that adverse effects on normal tissue can also be reduced.
Sato
H
H
Department of Orthopaedic Surgery, Yamanashi Medical College, Japan.
D000328:Adult; D000368:Aged; D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D001859:Bone Neoplasms / Q000188:drug therapy*; D002648:Child; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D008297:Male; D008748:Methylcholanthrene; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D012513:Sarcoma, Experimental / Q000139:chemically induced; D012983:Soft Tissue Neoplasms / Q000188:drug therapy*
D000970:Antineoplastic Agents; D008748:Methylcholanthrene