title: A morphological study of radiation nephropathy in the pig.
abstract: Both kidneys of mature pigs received a single dose of 9.8 Gy 60Co gamma rays. Pigs were killed between 2 and 24 weeks after irradiation and the kidneys examined histologically. Glomerular and tubular changes were observed within 2 weeks of irradiation. Neutrophils and other leukocytes were seen within glomerular capillary loops; mesangial matrix and cell number increased. A progressive increase in thickening of the basement membrane and a decrease in capillary lumina were then noted. Basement membrane duplication occurred within 12 weeks. By 24 weeks these lesions had increased in severity, sclerotic endstage glomeruli, predominantly subcapsular or juxtamedullary, being evident. Tubular lesions initially consisted of focal areas of tubular atrophy in the juxtamedullary region. By 6 weeks subcapsular foci of tubular degeneration, regeneration, and necrosis were found; these appeared to resolve 12 weeks after irradiation. At later times the severity of the tubular lesions varied between pigs, with some exhibiting interstitial fibrosis involving a complete band of subcapsular tissue, while others showed relatively mild changes. There was no apparent change in the vasculature. These findings indicate that (a) there is no one target or dose-limiting cell, and (b) the vasculature does not play a primary role in the development of radiation nephropathy.
authors.affiliation: CRC Normal Tissue Radiobiology Research Group, Research Institute (University of Oxford), Churchill Hospital, Headington, United Kingdom.
authors.forename: M E
authors.initials: ME
authors.lastname: Robbins
authors.forename: M J
authors.initials: MJ
authors.lastname: Wooldridge
authors.forename: R S
authors.initials: RS
authors.lastname: Jaenke
authors.forename: E
authors.initials: E
authors.lastname: Whitehouse
authors.forename: S J
authors.initials: SJ
authors.lastname: Golding
authors.forename: M
authors.initials: M
authors.lastname: Rezvani
authors.forename: J W
authors.initials: JW
authors.lastname: Hopewell
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000528:radiation effects; D003037:Cobalt Radioisotopes; D005260:Female; D005720:Gamma Rays; D005920:Glomerular Mesangium / Q000528:radiation effects; D007668:Kidney / Q000098:blood supply / Q000528:radiation effects*; D007674:Kidney Diseases / Q000209:etiology / Q000473:pathology*; D007678:Kidney Glomerulus / Q000528:radiation effects; D007684:Kidney Tubules / Q000528:radiation effects; D011833:Radiation Injuries, Experimental / Q000473:pathology*; D013552:Swine; D013997:Time Factors
chemical_list: D003037:Cobalt Radioisotopes