Glass-bead column separation of motile and nonmotile human spermatozoa.
Glass-bead columns were tested for their efficiency in concentrating motile human spermatozoa from frozen semen samples. The data show that glass-bead filtration concentrates the motile gametes in each sample and is significantly more efficient than the swim-up method for obtaining populations of motile spermatozoa. The data suggest that this method can be applied clinically to obtain motile spermatozoa from poor-quality semen for use in in vitro fertilization.
Lechtzin
N
N
Department of Biology, Temple University, Philadelphia, Pennsylvania 19122.
Garside
W
W
Heyner
S
S
Hillman
N
N
D002452:Cell Count; D002469:Cell Separation / Q000379:methods*; D015925:Cryopreservation; D005374:Filtration / Q000379:methods*; D005615:Freezing; D005898:Glass; D006801:Humans; D008297:Male; D013081:Sperm Motility; D013094:Spermatozoa