title: Effect of temperature on the metabolism of proteoglycans in explants of bovine articular cartilage.
abstract: The turnover of proteoglycans in bovine articular cartilage was determined in explant cultures, maintained at 32 degrees C or 37 degrees C. Both the rate of proteoglycan synthesis and the release of newly synthesized proteoglycans were decreased in cultures incubated at 32 degrees C compared to 37 degrees C. At both temperatures the newly synthesized proteoglycans were similar in hydrodynamic size and chain length of the glycosaminoglycans. However, the ratio of 6-sulfated disaccharides over 4-sulfated disaccharides of the newly synthesized glycosaminoglycans, differed less from the endogenous ratio at 32 degrees C than at 37 degrees C. At both temperatures, the incorporated 35S-sulfate is released from explants in two pools. Twenty-three percent of the 35S-radiolabel was released into the culture medium during an initial short phase (t1/2 = 1.1 day at 32 degrees C, 1.3 day at 37 degrees C), 77% had a much longer half-life. The lowered temperature markedly decreased the release of 35S-sulfate with a slow turnover (t1/2 = 60 days at 32 degrees C, 38 days at 37 degrees C).
authors.affiliation: Jan van Breemen Instituut, Centre for Rheumatology and Rehabilitation, Amsterdam, The Netherlands.
authors.forename: H S
authors.initials: HS
authors.lastname: Brand
authors.forename: M H
authors.initials: MH
authors.lastname: De Koning
authors.forename: G P
authors.initials: GP
authors.lastname: van Kampen
authors.forename: J K
authors.initials: JK
authors.lastname: Van der Korst
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000378:metabolism*; D002417:Cattle; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002808:Chondroitin Lyases / Q000378:metabolism; D046508:Culture Techniques; D005944:Glucosamine / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D006820:Hyaluronic Acid / Q000378:metabolism; D007700:Kinetics; D011509:Proteoglycans / Q000737:chemistry / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism; D013696:Temperature
chemical_list: D006025:Glycosaminoglycans; D011509:Proteoglycans; D013431:Sulfates; D006820:Hyaluronic Acid; D002808:Chondroitin Lyases; D005944:Glucosamine