title: Stimulation by D-glucose of the direct conversion of arginine to citrulline in enterocytes isolated from pig jejunum.
abstract: In enterocytes isolated from pig jejunum, L-arginine is metabolized to L-citrulline either directly or indirectly through the sequence of reactions catalysed by arginase and ornithine transcarbamylase. In the presence of 5 mM D-glucose, the direct conversion of 1mM L-[guanido-14C] arginine to L-citrulline was increased more than 4 times. Isolated enterocytes exhibit a high glycolytic capacity. Furthermore, the decarboxylation of 5mM D-[1-14C] glucose was 3.6 fold higher than the decarboxylation of 5 mM D-[6-14C] glucose which suggests the presence of a pentose phosphate pathway in enterocytes. Since the production of labelled L-citrulline from L-[guanido-14C] arginine in pig enterocyte homogenates was markedly increased in the presence of NADPH, it is proposed that the direct conversion of L-arginine to L-citrulline could be stimulated by the production of NADPH from D-glucose in the pentose phosphate pathway.
authors.affiliation: Laboratoire d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, JOUY-en-JOSAS, France.
authors.forename: F
authors.initials: F
authors.lastname: Blachier
authors.forename: H
authors.initials: H
authors.lastname: M'Rabet-Touil
authors.forename: B
authors.initials: B
authors.lastname: Darcy-Vrillon
authors.forename: L
authors.initials: L
authors.lastname: Posho
authors.forename: P H
authors.initials: PH
authors.lastname: Duee
mesh_terms: D000818:Animals; D001120:Arginine / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002956:Citrulline / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D007583:Jejunum / Q000187:drug effects / Q000378:metabolism*; D013552:Swine; D014508:Urea / Q000378:metabolism
chemical_list: D002250:Carbon Radioisotopes; D002956:Citrulline; D014508:Urea; D001120:Arginine; D005947:Glucose