title: Cyclosporine monitoring by fluorescence polarization immunoassay.
abstract: The monitoring of cyclosporine levels in whole blood and serum has become a routine procedure for the post-transplant management of immunosuppression. We have developed fluorescence polarization immunoassays for cyclosporine in whole blood and in serum using a monoclonal antibody. These assays are fast (20 determinations in less than 22 min), sensitive (25 micrograms/L for whole blood samples and 10 micrograms/L for serum samples), and precise (CV less than 7% in both assays). Cross-reactivities with AM1 (Metabolite 17) and AM4N (Metabolite 21) are less than 8.5 and 2.5%, respectively, for both assays.
authors.affiliation: Abbott Laboratories, Abbott Park, IL 60064.
authors.forename: P P
authors.initials: PP
authors.lastname: Wang
authors.forename: E
authors.initials: E
authors.lastname: Simpson
authors.forename: V
authors.initials: V
authors.lastname: Meucci
authors.forename: M
authors.initials: M
authors.lastname: Morrison
authors.forename: S
authors.initials: S
authors.lastname: Lunetta
authors.forename: M
authors.initials: M
authors.lastname: Zajac
authors.forename: R
authors.initials: R
authors.lastname: Boeckx
mesh_terms: D000911:Antibodies, Monoclonal; D001322:Autoanalysis; D003524:Cyclosporins / Q000097:blood*; D016231:Fluorescence Polarization Immunoassay / Q000379:methods; D006801:Humans; D016030:Kidney Transplantation / Q000502:physiology*; D008991:Monitoring, Physiologic / Q000379:methods; D015203:Reproducibility of Results
chemical_list: D000911:Antibodies, Monoclonal; D003524:Cyclosporins