Inhibition of elastase enzyme release from human polymorphonuclear leukocytes by N-acetyl-galactosamine and N-acetyl-glucosamine.
We studied the effect of aminosugars on the elastase enzyme released from cytochalasin-B treated polymorphonuclear neutrophils (PMN) and stimulated by F-Met-Leu-Phe (FMLP). Both N-acetyl-galactosamine and N-acetyl-glucosamine can inhibit the release of leukocyte elastase enzyme. N-acetyl-galactosamine was a potent inhibitor of elastase enzyme release in a dose-related fashion. Inhibition ranged from 91.9% up to total inhibition. N-acetyl-glucosamine showed a mild inhibitory effect on elastase enzyme release; however, it too acted in a dose-related fashion. The range of inhibition was 17.17%-8.18%. Mannosamine showed an inhibitory effect in some donors, but this effect was not statistically significant. No effect on elastase enzyme release was shown with other sugars such as D-glucose and L-fucose. These results show that N-acetyl-galactosamine can inhibit the respiratory burst, which suggests that the aminosugars might do more than interfere with the carbohydrate lectin interaction.
Kamel
M
M
Department of Rheumatology, Azhar University, Cairo, Egypt.
Hanafi
M
M
Bassiouni
M
M
D000116:Acetylgalactosamine / Q000494:pharmacology*; D000117:Acetylglucosamine / Q000494:pharmacology*; D000328:Adult; D001172:Arthritis, Rheumatoid / Q000201:enzymology; D003571:Cytochalasin B / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology; D010196:Pancreatic Elastase / Q000378:metabolism*
D003571:Cytochalasin B; D009240:N-Formylmethionine Leucyl-Phenylalanine; D010196:Pancreatic Elastase; D000116:Acetylgalactosamine; D000117:Acetylglucosamine