Nucleotide sequence of the replication region of the marine Rhodobacter plasmid pRD31.
The minimum region required for replication of the marine Rhodobacter endogenous plasmid pRD31 has been sequenced. This region is located on a 1367-bp HincII-PstI restriction fragment and is 62% rich in G-C base pairs. A region homologous to the bacteriophage P1 repA promoter, which overlaps two inverted repeats, has been identified. Plasmids with mutations in this 1367-bp region could not replicate in marine Rhodobacter hosts. This is the first identified replication origin of a photosynthetic bacterium.
Matsunaga
T
T
Department of Biotechnology, Tokyo University of Agriculture and Technology, Japan.
Mihashita
H
H
Miyake
M
M
Burgess
J G
JG
D000595:Amino Acid Sequence; D001483:Base Sequence; D004261:DNA Replication; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012241:Rhodopseudomonas / Q000235:genetics*; D012623:Seawater