Cyclosporine monitoring by fluorescence polarization immunoassay.
The monitoring of cyclosporine levels in whole blood and serum has become a routine procedure for the post-transplant management of immunosuppression. We have developed fluorescence polarization immunoassays for cyclosporine in whole blood and in serum using a monoclonal antibody. These assays are fast (20 determinations in less than 22 min), sensitive (25 micrograms/L for whole blood samples and 10 micrograms/L for serum samples), and precise (CV less than 7% in both assays). Cross-reactivities with AM1 (Metabolite 17) and AM4N (Metabolite 21) are less than 8.5 and 2.5%, respectively, for both assays.
Wang
P P
PP
Abbott Laboratories, Abbott Park, IL 60064.
Simpson
E
E
Meucci
V
V
Morrison
M
M
Lunetta
S
S
Zajac
M
M
Boeckx
R
R
D000911:Antibodies, Monoclonal; D001322:Autoanalysis; D003524:Cyclosporins / Q000097:blood*; D016231:Fluorescence Polarization Immunoassay / Q000379:methods; D006801:Humans; D016030:Kidney Transplantation / Q000502:physiology*; D008991:Monitoring, Physiologic / Q000379:methods; D015203:Reproducibility of Results
D000911:Antibodies, Monoclonal; D003524:Cyclosporins