title: Preliminary characterization and partial purification of rat uterine nuclear type II Binding sites.
abstract: These studies represent the first biochemical characterization and purification of nuclear type II binding sites from the rat uterus. Uterine nuclei from estradiol-implanted rats were digested with DNA'se and RNA'se, washed with Na deoxycholate-Tween 40 and extracted with 0.4 M ammonium sulfate (AmSO4). Nuclear type II sites in the AmSO4 extract eluted as a single peak during DEAE ion exchange chromatography, HPLC (Waters DEAE 5PW column) and Sephadex G-100 chromatography with a molecular weight of approximately 37K. DEAE and quercetin-sepharose affinity chromatography resulted in significant purification (greater than 800-fold) of nuclear type II sites with a 49% yield. Type II sites were not recognized by rat ER antibodies (Abbot ER-EIA kit) which immunoadsorbed ER from these preparations. These biochemical and immunological studies suggest that the ER and type II sites are likely to be different proteins.
authors.affiliation: Center for Biotechnology, Baylor College of Medicine, The Woodlands, Texas 77381.
authors.forename: B M
authors.initials: BM
authors.lastname: Markaverich
authors.forename: R R
authors.initials: RR
authors.lastname: Gregory
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D007118:Immunoassay; D007700:Kinetics; D010052:Ovariectomy; D051381:Rats; D011919:Rats, Inbred Strains; D011960:Receptors, Estrogen / Q000302:isolation & purification / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism*
chemical_list: D011960:Receptors, Estrogen; D004958:Estradiol