title: The cis-acting regulatory element of the mvaAB operon of Pseudomonas mevalonii.
abstract: DNA upstream of the transcription start site of the mvaAB operon of Pseudomonas mevalonii, which encodes 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase (EC 1.1.1.88) and HMG-CoA lyase (EC 4.1.3.4), contains a cis-acting regulatory element which functions in the response to mevalonate. The regulatory element resides within a 36-bp region located from 48 to 84 bp upstream of the transcription start site of mvaA. This location was inferred from the beta-galactosidase activities of P. mevalonii harboring plasmid-encoded mvaA-lacZ fusions induced by mevalonate and by DNA gel retardation and competition assays. While protein from P. mevalonii grown on mevalonate produced a band shift, protein from cells grown on succinate gave no band shift, even when mevalonate was added. The operator contains three 10-bp direct repeats with the consensus sequence TGGGTACAGT, which may be important for regulation of the mvaAB operon.
authors.affiliation: Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907.
authors.forename: Y L
authors.initials: YL
authors.lastname: Wang
authors.forename: V W
authors.initials: VW
authors.lastname: Rodwell
mesh_terms: D001483:Base Sequence; D001667:Binding, Competitive; D015342:DNA Probes; D004269:DNA, Bacterial / Q000378:metabolism; D005798:Genes, Bacterial; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics; D008969:Molecular Sequence Data; D009876:Operon; D010957:Plasmids; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D006903:Hydroxymethylglutaryl CoA Reductases