High-performance liquid chromatographic evaluation of 2-(alpha-thenoylthio)propionylglycine and its two metabolites in biological fluids.
A high-performance liquid chromatographic (HPLC) method for determining 2-(alpha-thenoylthio)propionylglycine (TTPG) and its two main metabolites, thiophenecarboxylic acid and thiopronine, in biological samples was developed. TTPG and its metabolites were extracted by solvent partition and then determined by reversed-phase HPLC with UV detection at 245, 295 and 360 nm. This procedure was validated in order to allow the assay of these compounds in plasma and urine samples with sufficiently low detection limits (50 ng/ml for TTPG and TCA and 100 ng/ml for thiopronine) and with good linearity within the concentration range investigated. It was applied to a comprehensive pharmacokinetic investigation of TTPG in healthy volunteers.
Marzo
A
A
Sigma-Tau SpA, Rome, Italy.
Arrigoni Martelli
E
E
Bruno
G
G
Nava
D
D
Mignot
A
A
Vidal
R
R
Lefebvre
M A
MA
D002851:Chromatography, High Pressure Liquid; D005998:Glycine / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism / Q000652:urine; D006801:Humans; D012016:Reference Values; D013056:Spectrophotometry, Ultraviolet; D013440:Sulfides; D013876:Thiophenes
D013440:Sulfides; D013876:Thiophenes; C035227:stepronin; D005998:Glycine