Indomethacin reverses interleukin-1-induced hyperinsulinemia in conscious and freely moving rats.
With the aim of studying the mechanism by which peripherally injected interleukin-1 increases the serum levels of insulin in conscious rats, the effects of indomethacin, a cyclooxygenase inhibitor, on interleukin-1-induced hyperinsulinemia were examined using male Wistar rats implanted with intra-atrial cannulas. The i.v. injection of interleukin-1 (1 microgram/rat) into conscious rats resulted in a significant increase in serum insulin levels with the peak response being observed 10 min after injection. It was also found that this insulin response to interleukin-1 was dose-related. Pretreatment with indomethacin (3 mg/kg body weight), however, completely blocked the interleukin-1-induced stimulation of insulin secretion. These findings suggest that i.v. injected interleukin-1 stimulates insulin release from the endocrine pancreas by a mechanism involving the prostaglandin system.
Uehara
A
A
Department of Internal Medicine (III), Asahikawa Medical College, Hokkaido, Japan.
Okumura
T
T
Kumei
Y
Y
Takasugi
Y
Y
Namiki
M
M
D000758:Anesthesia; D000818:Animals; D004305:Dose-Response Relationship, Drug; D006946:Hyperinsulinism / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy*; D007213:Indomethacin / Q000494:pharmacology*; D007328:Insulin / Q000097:blood; D007375:Interleukin-1 / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D007375:Interleukin-1; D007213:Indomethacin