Augmentation of granulocyte/macrophage colony-stimulating factor expression by ultraviolet irradiation is mediated by interleukin 1 in Pam 212 keratinocytes.
Keratinocytes are a potent source of a variety of cytokines including granulocyte-macrophage colony-stimulating factor (GM-CSF). In this study, we have shown that ultraviolet B (UVB) irradiation augments GM-CSF mRNA expression by murine keratinocytes. This is reflected in the increased production of GM-CSF protein by these cells. In the same cell population, exposure to UVB irradiation increases interleukin 1 alpha (IL-1 alpha) mRNA and IL-1 protein as detected by bioactivity. This increase in IL-1 alpha precedes the increase of GM-CSF mRNA. Addition of recombinant IL-1 alpha to the medium increases GM-CSF mRNA expression. Anti-IL-1 alpha antibodies can completely inhibit UV-augmented GM-CSF mRNA expression. These results demonstrate that UVB irradiation-induced augmentation of GM-CSF is mediated by UV-induced IL-1 alpha.
Nozaki
S
S
Department of Dermatology, Showa University, Tokyo, Japan.
Abrams
J S
JS
Pearce
M K
MK
Sauder
D N
DN
D000818:Animals; D002460:Cell Line; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D007375:Interleukin-1 / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D015603:Keratinocytes / Q000378:metabolism / Q000528:radiation effects*; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis; D014466:Ultraviolet Rays
D007375:Interleukin-1; D012333:RNA, Messenger; D016178:Granulocyte-Macrophage Colony-Stimulating Factor