prp4 from Schizosaccharomyces pombe, a mutant deficient in pre-mRNA splicing isolated using genes containing artificial introns.
We have generated a bank of temperature-sensitive (ts) Schizosaccharomyces pombe mutant strains. About 150 of these mutants were transformed with a ura4 gene containing an artificial intron. We screened these ts mutants for mutants deficient in splicing of the ura4 intron. With this approach three mutants were isolated which have a general defect in the splicing process. Two of these mutants fall into the prp1 complementation group and one defines a new complementation group, prp4.
Rosenberg
G H
GH
Department of Bioscience and Biotechnology, Drexel University, Philadelphia, PA 19104.
Alahari
S K
SK
Käufer
N F
NF
D005800:Genes, Fungal; D005813:Genes, Synthetic; D005816:Genetic Complementation Test; D007438:Introns; D009154:Mutation; D012322:RNA Precursors / Q000378:metabolism*; D012326:RNA Splicing / Q000235:genetics*; D012331:RNA, Fungal / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000235:genetics* / Q000302:isolation & purification; D013696:Temperature
D012322:RNA Precursors; D012331:RNA, Fungal