Short-term primary cultures in studies of growth regulation in rat proximal tubule cells.
Recent methodological developments have made primary cultures of renal cells a promising model for studies of renal growth regulation. By minimal culture time and single-cell techniques, homogenous populations of highly differentiated cells can be studied. This report reviews the model and recent studies on growth regulation in rat proximal tubule cells (PTC). It is demonstrated that no correlation exists between proliferative rate and intracellular pH (pHi) in PTC, a topic of previous debate. The rate of proliferation in cultured PTC is similar to in vivo and dependent on the postnatal age of the animal. Recent evidence demonstrates that the model is an important tool for studies of postnatal changes in renal growth regulation.
Larsson
S H
SH
Department of Pediatrics, St. Göran's Children's Hospital, Karolinska Institutet, Stockholm, Sweden.
D000818:Animals; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured / Q000166:cytology / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D007687:Kidney Tubules, Proximal / Q000166:cytology* / Q000254:growth & development; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors