Cell cycle-specific expression and nuclear binding of DNA polymerase alpha.
The expression and distribution of DNA polymerase alpha was measured by cytometry and confocal laser scanning microscopy. Expression was proportional to DNA content in proliferating cells, while only S-phase cells retained DNA polymerase alpha after detergent extraction. Nuclear DNA polymerase alpha binding may be one of the key events of S-phase entry.
Stokke
T
T
Department of Biophysics, Norwegian Radium Hospital, Oslo.
Erikstein
B
B
Holte
H
H
Funderud
S
S
Steen
H B
HB
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000201:enzymology; D002453:Cell Cycle; D002460:Cell Line; D002467:Cell Nucleus / Q000502:physiology* / Q000648:ultrastructure; D002478:Cells, Cultured; D004257:DNA Polymerase II / Q000378:metabolism*; D005434:Flow Cytometry; D016193:G1 Phase; D006801:Humans; D007834:Lasers; D016196:S Phase
D004257:DNA Polymerase II