Uridine enhances expression of the fragile X chromosome in human lymphocytes.
We have studied the effect of uridine on the expression of fragile X (fra[X]) in lymphocyte cultures established in the folate and thymidine deficient medium TC199. The results indicate that uridine enhances the expression of fra(X) and gives a higher mitotic rate. The excess of uridine during DNA synthesis might further promote the previously suggested cycle of misincorporation and removal of deoxyuridine monophosphate when the pool of deoxythymidine triphosphate is continuously depleted.
Kähkönen
M
M
Department of Clinical Genetics, Oulu University Central Hospital, Finland.
Haataja
R
R
Leisti
J
J
D003470:Culture Media; D005492:Folic Acid / Q000032:analysis; D005600:Fragile X Syndrome / Q000235:genetics*; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry*; D008938:Mitosis; D013936:Thymidine / Q000032:analysis; D014529:Uridine / Q000737:chemistry*
D003470:Culture Media; D005492:Folic Acid; D013936:Thymidine; D014529:Uridine