title: Cis-regulatory elements that confer differential expression upon the rat gene encoding phosphoenolpyruvate carboxykinase in kidney and liver.
abstract: The PCK gene, encoding cytosolic phosphoenolpyruvate carboxykinase, is specifically expressed in gluconeogenic tissues, liver and kidney. Hence it serves as a model of a class of single-copy genes whose transcription is restricted to a few tissues, rather than a unique tissue. To begin delineating the mechanisms that govern this pattern of expression, cis-regulatory elements of PCK were examined using transient transfection assays in PCK-expressing kidney and hepatoma cell lines. The analyses enabled us to identify a proximal element, between nucleotide (nt) positions -121 and -98, relative to the transcription start point that is sufficient for specific expression in kidney cells, but is just one of the elements required for expression in hepatoma cells. A distal element (between nt -487 and -417), which is essential for hepatoma-specific expression, is not needed in kidney cells. We suggest that the differential regulation of PCK expression in the liver and kidney results from an interplay between different cis-regulatory elements and trans-acting factors.
authors.affiliation: Department of Developmental Biochemistry, Hebrew University Hadassah Medical School, Jerusalem, Israel.
authors.forename: T
authors.initials: T
authors.lastname: Shoshani
authors.forename: N
authors.initials: N
authors.lastname: Benvenisty
authors.forename: M
authors.initials: M
authors.lastname: Trus
authors.forename: L
authors.initials: L
authors.lastname: Reshef
mesh_terms: D000818:Animals; D022762:CCAAT-Enhancer-Binding Proteins; D002460:Cell Line; D002872:Chromosome Deletion; D003600:Cytosol; D004268:DNA-Binding Proteins / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008114:Liver Neoplasms, Experimental; D009154:Mutation / Q000502:physiology; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D010730:Phosphoenolpyruvate Carboxylase / Q000235:genetics*; D011401:Promoter Regions, Genetic; D051381:Rats; D013552:Swine; D015534:Trans-Activators / Q000502:physiology; D014157:Transcription Factors / Q000502:physiology*; D014162:Transfection; D014407:Tumor Cells, Cultured; D051840:Y-Box-Binding Protein 1
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human; D010730:Phosphoenolpyruvate Carboxylase