title: Crystallization and preliminary diffraction analysis of cholesterol esterase from Candida cylindracea.
abstract: Cholesterol esterase (EC 3.1.1.13) from the microorganism Candida cylindracea has been crystallized in two forms. Crystals, typically 0.30 x 0.15 x 0.10 mm in size, diffract rotating anode generated x-rays to beyond 3 A are suitable for data collection for an x-ray crystallographic investigation. A monoclinic crystal form in the space group P2(1) was found to have cell dimensions of a = 122.9 A, b = 101.0 A, c = 95.2 A and beta = 108.3 degrees. The asymmetric unit of the cell contains two dimers of 129 kDa each. A second crystal form, in the triclinic space group P1, has cell dimensions of a = 58.6 A, b = 88.7 A, c = 58.6 A, alpha = 93.3 degrees, beta = 113.8 degrees and gamma = 96.0 degrees, and has one dimer per asymmetric unit.
authors.affiliation: Medical Foundation of Buffalo, Inc., NY 14203-1196.
authors.forename: D
authors.initials: D
authors.lastname: Ghosh
authors.forename: M
authors.initials: M
authors.lastname: Erman
authors.forename: W L
authors.initials: WL
authors.lastname: Duax
mesh_terms: D002175:Candida / Q000201:enzymology*; D003460:Crystallization; D002787:Sterol Esterase / Q000737:chemistry / Q000302:isolation & purification*; D014961:X-Ray Diffraction
chemical_list: D002787:Sterol Esterase