Fibropellins, products of an EGF repeat-containing gene, form a unique extracellular matrix structure that surrounds the sea urchin embryo.
The sea urchin SpEGF 1 gene belongs to a growing family of developmentally important genes which encode proteins that contain repeated epidermal growth factor-like motifs. To characterize the embryonic expression of the protein products of this gene from Strongylocentrotus purpuratus, we generated polyclonal antisera from SpEGF I fusion proteins. These antibodies recognize two glycoproteins of 145 and 185 kDa, which we have named fibropellins. These proteins are present in unfertilized oocytes and throughout early development. The fibropellins are stored in cytoplasmic vesicles in the oocyte and are released soon after fertilization in a distinct secretory event following the exocytosis of cortical granule contents. Following secretion the proteins are localized in the basal surface of the hyaline layer. At the blastula stage the fibropellins become organized into distinct fibers which form a mesh-like network over the surface of the embryo. During subsequent development to the pluteus larva stage this network increases in overall morphological complexity and becomes regionally distinct. The molecular weights of the fibropellins and their pattern of embryonic localization indicate that these proteins form a component of the hyaline layer previously described as the apical lamina.
Bisgrove
B W
BW
Institute for Molecular and Cellular Biology, Indiana University, Bloomington 47405.
Andrews
M E
ME
Raff
R A
RA
D000818:Animals; D000918:Antibody Specificity; D001755:Blastocyst / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D005109:Extracellular Matrix / Q000378:metabolism / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D006023:Glycoproteins / Q000096:biosynthesis / Q000235:genetics; D008970:Molecular Weight; D005810:Multigene Family; D012617:Sea Urchins / Q000196:embryology*
D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D004815:Epidermal Growth Factor