Effect of nutrients on defined bacterial plaques and Streptococcus mutans C67-1 implantation in a model mouth.
Actinomyces viscosus WVU 627, Streptococcus oralis LPA-1 and Veillonella dispar OMZ 193 were cocultured on teeth in a model mouth for 66 h. Synthetic saliva containing bovine salivary glycoprotein supported bacterial growth, although the delivery of an intermittent nutrient supplement, containing 1% (w/v) glucose or sucrose, gave greater bacterial cell and viable counts. When Streptococcus mutans C67-1 was super-inoculated onto 24-hour mixed plaques, it became established under all regimens, but there was pronounced colonization resistance. With saliva only, the proportion of S. mutans at 66 h was less than 0.5% of the total cultivable microflora. When a glucose supplement was delivered for 1 h every 6 h, S. mutans attained a final proportion of 2.4%. With sucrose, both S. mutans C67-1 and its non-cariogenic glucan-deficient mutant, C67-25, attained similar proportions of 15-20%. These experiments indicate how this model can be used to study the factors influencing colonizing ability and microbial interactions in biofilms under controlled conditions.
Donoghue
H D
HD
University College and Middlesex School of Dentistry, London, England.
Perrons
C J
CJ
D000190:Actinomyces / Q000254:growth & development / Q000378:metabolism; D000898:Antibiosis; D015169:Colony Count, Microbial; D003470:Culture Media; D003773:Dental Plaque / Q000382:microbiology*; D004463:Ecology; D005947:Glucose / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D008954:Models, Biological; D012464:Saliva, Artificial / Q000378:metabolism; D012471:Salivary Proteins and Peptides / Q000378:metabolism; D013291:Streptococcus / Q000254:growth & development / Q000378:metabolism; D013295:Streptococcus mutans / Q000254:growth & development* / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism; D014678:Veillonella / Q000254:growth & development / Q000378:metabolism
D003470:Culture Media; D006023:Glycoproteins; D012464:Saliva, Artificial; D012471:Salivary Proteins and Peptides; D013395:Sucrose; D005947:Glucose