[Immune status evaluation in the small amount of peripheral blood in children with chronic inflammatory diseases of the upper respiratory tract].
This paper describes microtechniques for studying basic immune parameters which require small amounts of peripheral blood. These techniques include: relative (as a percentage) and absolute (per microliter) measurement of T-lymphocytes using the method of spontaneous rosette formation (SRF) with sheep erythrocytes (SE); B-lymphocytes using SRF with mouse erythrocytes; capability of band neutrophils for SRF with SE; and phagocytic activity of band neutrophils toward the Staphylococcus aureus strain under study in the autoserum reaction and with healthy children serum to detect potential changes in the opsonic activity of the patient's serum. Our measurements have demonstrated that the double density gradient method to measure lymphocytes and neutrophil bands in a small amount of blood makes it possible to determine immune parameters and reveal primary and secondary immune deficiencies. This is also supported by the fact that results derived from small and large amounts of blood of these children are identical. These measurements are relatively simple and not too time-consuming. All this shows that the above methods can be used for assessing the immune status of children with chronic upper airways diseases.
Lazarev
V N
VN
Skriabin
A S
AS
Pekli
F F
FF
D000293:Adolescent; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D006801:Humans; D007111:Immunity, Cellular; D010586:Phagocytes / Q000276:immunology; D012852:Sinusitis / Q000097:blood / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014069:Tonsillitis / Q000097:blood / Q000276:immunology*
Otsenka immunnogo statusa v malom ob'eme perifericheskoĭ krovi u deteĭ s khronicheskimi vospalitel'nymi zabolevaniami verkhnikh dykhatel'nykh puteĭ.