Application of glycerol to freezing bovine pancreas. Part 2. Effects of glycerol and grinding on frozen pancreas quality.
Effects of glycerol on the quality of ground and whole pancreas stored frozen at -30 degrees C were studied. The quality was assessed from quantitative changes in soluble proteins, proteolytic enzyme activity, and insulin content. Grinding was found to enhance the effects of the cryoprotector due to its uniform distribution in the tissue. A relationship between proteolytic enzyme activity and protein solubility was demonstrated.
Hajduk
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Department of Refrigeration and Food Industry Engineering, Agricultural University, Cracow, Poland.
Fik
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M
D000818:Animals; D002417:Cattle; D015925:Cryopreservation; D010450:Endopeptidases / Q000032:analysis; D005990:Glycerol / Q000494:pharmacology*; D007328:Insulin / Q000032:analysis; D010179:Pancreas / Q000737:chemistry / Q000187:drug effects*; D011506:Proteins / Q000032:analysis; D012995:Solubility; D013997:Time Factors
D007328:Insulin; D011506:Proteins; D010450:Endopeptidases; D005990:Glycerol