Validation of a toxicity testing model by evaluating oxygen supply and energy state in the isolated perfused rat kidney. Single-pass preparation without albumin.
Validation of a toxicity testing model concerning energy metabolism was attempted by evaluating the oxygen supply and energy state in an isolated perfused rat kidney of single-pass preparation without albumin. Perfusion was performed at a temperature of 31 degrees C, flow rate of 11.0 mL/g/min, and pressure of 81-104 mmHg. The perfusate was saturated with 95% O2/5% CO2. After preperfusion for 30 min, the redox states of cytochrome aa3 and c and pyridine nucleotides (PN) in the perfused kidney were measured to be stable for 90 min by a scanning reflectance spectrophotometry and surface fluorometry, respectively. During the same period, the contents of ATP and inorganic phosphate (Pi) in the perfused kidney were also measured to be stable by 31P-NMR spectroscopy. The oxygen supply to the cell was more than the amount required for the basal metabolism of the cell. For assessment of the effects of chemical agents on the renal cell metabolism, this preparation of the perfused rat kidney was considered to have several advantages, despite some of its inherent limitations in the function of the kidney.
Takano
T
T
Department of Public Health and Environmental Science, School of Medicine, Tokyo Medical and Dental University, Japan.
Nakata
K
K
Kawakami
T
T
Miyazaki
Y
Y
Murakami
M
M
Seo
Y
Y
Suzuki
E
E
D000255:Adenosine Triphosphate / Q000378:metabolism; D000418:Albumins / Q000494:pharmacology; D000818:Animals; D004734:Energy Metabolism; D005260:Female; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010101:Oxygen Consumption; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D014116:Toxicology / Q000379:methods*
D000418:Albumins; D000255:Adenosine Triphosphate