Screening of clones containing altered DNA conformations by plasmid topoprofile shift.
DNA topoprofile of supercoiled plasmid is influenced by the presence of altered structures in some stretches. We describe a non-radioactive method for screening the clones containing such altered structures.
Ramesh
N
N
Molecular Biophysics Unit, Indian Institute of Science, Bangalore.
Mishra
R K
RK
D001483:Base Sequence; D003001:Cloning, Molecular; D004270:DNA, Circular / Q000737:chemistry; D004278:DNA, Superhelical / Q000737:chemistry*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000235:genetics*
D004270:DNA, Circular; D004278:DNA, Superhelical