title: A high-performance liquid chromatographic method for the measurement of the iron chelator 1,2-dimethyl-3-hydroxypyridin-4-one in human plasma.
abstract: 1,2-Dimethyl-3-hydroxypyridin-4-one (CP020 or L1) is a novel oral iron chelator that has proved to be effective in animals and humans. A rapid, accurate, and sensitive high-performance liquid chromatography method is described for measuring L1 in human plasma using a Hypercarb 7 microns column and monitoring the column eluent by ultraviolet absorption at 280 nm. CP020 and the internal standard (CP094) were extracted into dichloromethane (2 x 5 ml) from plasma at neutral pH [0.25 ml of plasma + 0.75 ml of 60 mM 3-(N-morpholino)propanesulfonic acid buffer, pH 7.4]. The method proved to be linear (r2 = 0.998) in the clinical range of 0.5-50 micrograms/ml when 0.25 ml of plasma was used, with the coefficient of variation less than 10% even at the lower concentration range.
authors.affiliation: Department of Pharmacy, King's College, London, U.K.
authors.forename: J
authors.initials: J
authors.lastname: Klein
authors.forename: L A
authors.initials: LA
authors.lastname: Damani
authors.forename: D
authors.initials: D
authors.lastname: Chung
authors.forename: O
authors.initials: O
authors.lastname: Epemoulu
authors.forename: N
authors.initials: N
authors.lastname: Olivieri
authors.forename: G
authors.initials: G
authors.lastname: Koren
mesh_terms: D000293:Adolescent; D002648:Child; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000077543:Deferiprone; D006801:Humans; D007502:Iron Chelating Agents / Q000378:metabolism*; D011728:Pyridones / Q000097:blood*; D013789:Thalassemia / Q000097:blood
chemical_list: D007502:Iron Chelating Agents; D011728:Pyridones; D000077543:Deferiprone