title: Production of bovine insulin-like growth factor 2 (bIGF2) in Escherichia coli.
abstract: Bovine insulin-like growth factor 2 (bIGF2) was produced in inclusion bodies in the cytoplasm of Escherichia coli and accumulated at high levels: 20-25% of total Coomassie-stained bacterial protein. The level of accumulation of bIGF2 was affected by the choice of codons in the 5' end of the coding sequence and by a rpoH mutation in the host cells. Purified recombinant bIGF2 had the native N terminus and the same mitogenic activity as that of bIGF2 purified from bovine serum.
authors.affiliation: Department of Biological Sciences, Monsanto Co., Chesterfield, MO 63198.
authors.forename: A M
authors.initials: AM
authors.lastname: Easton
authors.forename: J K
authors.initials: JK
authors.lastname: Gierse
authors.forename: R
authors.initials: R
authors.lastname: Seetharam
authors.forename: B K
authors.initials: BK
authors.lastname: Klein
authors.forename: C E
authors.initials: CE
authors.lastname: Kotts
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics; D002455:Cell Division / Q000187:drug effects; D003062:Codon; D003593:Cytoplasm / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D007335:Insulin-Like Growth Factor II / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D021382:Protein Sorting Signals / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*
chemical_list: D003062:Codon; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D007335:Insulin-Like Growth Factor II