The diagnostic value of immunoperoxidase in detecting K99+ Escherichia coli on histological sections.
The small intestine of 51 calves was examined for the presence of K99+ Escherichia coli by means of both an immunoperoxidase procedure performed on paraffin sections and by the slide agglutination test after isolation. Twelve cases resulted immunoperoxidase positive (23.5%) and 8 of them were also agglutination positive. Results of the 2 diagnostic tests agreed in 46 cases (90.2%). In immunoperoxidase positive sections a thick layer of immunoreactive bacteria was seen on the luminal surface of the enterocytes. Post-mortem autolysis or prolonged fixation did not seem to affect the immunoperoxidase reactivity of the sample.
Scanziani
E
E
Istituto di Anatomia Patologica Veterinaria e Patologia Aviare, Università degli Studi, Milan, Italy.
Luini
M
M
Crippa
L
L
Nigrelli
A D
AD
Lavazza
A
A
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D003967:Diarrhea / Q000175:diagnosis / Q000662:veterinary*; D004926:Escherichia coli / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000175:diagnosis / Q000662:veterinary*; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000382:microbiology