Improved measurement of urinary catecholamines by liquid-liquid extraction, derivatization and high-performance liquid chromatography with fluorimetric detection.
We report a sensitive and reliable assay for the determination of the urinary catecholamines norepinephrine, epinephrine and dopamine, based on selective extraction by a liquid-liquid extraction procedure, followed by selective derivatization with the fluorigenic agent 1,2-diphenylethylenediamine and quantification by high-performance liquid chromatography with fluorimetric detection. Comparison with a method using electrochemical detection shows that interference of an unknown compound, most probably N-methylepinephrine, which is an often-overlooked problem with methods using electrochemical detection and results in falsely high epinephrine concentrations, does not occur with the described fluorimetric method.
van der Hoorn
F A
FA
Department of Internal Medicine I, University Hospital Dijkzigt, Erasmus University, Rotterdam, The Netherlands.
Boomsma
F
F
Man in 't Veld
A J
AJ
Schalekamp
M A
MA
D002395:Catecholamines / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry; D005470:Fluorometry; D006801:Humans; D009638:Norepinephrine / Q000652:urine
D002395:Catecholamines; D009638:Norepinephrine