Thinning of the placental villous membrane during maintenance in hypoxic organ culture: structural adaptation or syncytial degeneration?
This study was undertaken in order to reinvestigate claims that the human placenta is able to adapt structurally under hypoxic stress so as to facilitate materno-fetal oxygen exchange. The results confirmed that thinning of the villous membrane does occur after maintenance for 10 h in an atmosphere of 6% oxygen, and that this effect is partially reversed by maintenance for a further 10 h under normoxic conditions (21% O2). However, throughout these experiments the values for both the arithmetic and the harmonic mean thicknesses of the villous membrane altered in an identical fashion. This indicates that the thinning observed was not the result of redistribution of the syncytiotrophoblast around the villous surface, with movement away from the sites of the fetal capillaries as previously postulated. Rather, it must have been caused by a reduction in the total mass of the syncytium, resulting from either involution and partial degeneration of its organelles, or some form of fluid-shift phenomenon. Whatever the cause, these effects were only partially reversed after further culture for 10 h in well-oxygenated conditions. Thus whilst there is an accumulating body of evidence to suggest that the placenta can adapt structurally to hypoxia in vivo, it is apparent that the in vitro organ culture system is not a suitable model with which to investigate the process.
Ong
P J
PJ
Department of Anatomy, University of Cambridge, U.K.
Burton
G J
GJ
D000704:Analysis of Variance; D002196:Capillaries / Q000187:drug effects; D015687:Cell Hypoxia; D002824:Chorionic Villi / Q000378:metabolism*; D005260:Female; D005311:Fetal Hypoxia / Q000503:physiopathology*; D015726:Giant Cells / Q000378:metabolism; D006801:Humans; D009924:Organ Culture Techniques; D010100:Oxygen / Q000378:metabolism*; D013997:Time Factors; D014327:Trophoblasts / Q000378:metabolism
D010100:Oxygen