title: Rapid DNA sequencing based upon single molecule detection.
abstract: We are developing a laser-based technique for the rapid sequencing of 40-kb or larger fragments of DNA at a rate of 100 to 1000 bases per second. The approach relies on fluorescent labeling of the bases in a single fragment of DNA, attachment of this labeled DNA fragment to a support, movement of the supported DNA fragment into a flowing sample stream, and detection of individual fluorescently labeled bases as they are cleaved from the DNA fragment by an exonuclease. The ability to sequence large fragments of DNA will significantly reduce the amount of subcloning and the number of overlapping sequences required to assemble megabase segments of sequence information.
authors.affiliation: Center for Human Genome Studies, Los Alamos National Laboratory, NM 87545.
authors.forename: L M
authors.initials: LM
authors.lastname: Davis
authors.forename: F R
authors.initials: FR
authors.lastname: Fairfield
authors.forename: C A
authors.initials: CA
authors.lastname: Harger
authors.forename: J H
authors.initials: JH
authors.lastname: Jett
authors.forename: R A
authors.initials: RA
authors.lastname: Keller
authors.forename: J H
authors.initials: JH
authors.lastname: Hahn
authors.forename: L A
authors.initials: LA
authors.lastname: Krakowski
authors.forename: B L
authors.initials: BL
authors.lastname: Marrone
authors.forename: J C
authors.initials: JC
authors.lastname: Martin
authors.forename: H L
authors.initials: HL
authors.lastname: Nutter
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D005090:Exodeoxyribonucleases / Q000378:metabolism; D005456:Fluorescent Dyes; D005821:Genetic Techniques / Q000295:instrumentation; D007834:Lasers; D009711:Nucleotides / Q000032:analysis
chemical_list: D005456:Fluorescent Dyes; D009711:Nucleotides; D004247:DNA; D005090:Exodeoxyribonucleases