title: Isolation and characterization of maltose non utilizing (mnu) mutants mapping outside the MAL1 locus in Saccharomyces cerevisiae.
abstract: The MAL1 locus of Saccharomyces cerevisiae comprises three genes necessary for maltose utilization. They include regulatory, maltose transport and maltase genes designated MAL1R, MAL1T and MAL1S respectively. Using a MAL1 strain transformed with an episomal, multicopy plasmid carrying the MAL2 locus, five recessive and one dominant mutant unable to grow on maltose, but still retaining a functional MAL1 locus were isolated. All the mutants could use glycerol, ethanol, raffinose and sucrose as a sole carbon source; expression of the maltase and maltose permease genes was severely and coordinately reduced. Only the dominant mutant failed to accumulate the MAL1R mRNA.
authors.affiliation: Dipartimento Fisiologia e Biochimica Generali, Università degli Studi di Milano, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Vanoni
authors.forename: M J
authors.initials: MJ
authors.lastname: Goldenthal
mesh_terms: D015152:Blotting, Northern; D005800:Genes, Fungal; D005838:Genotype; D008320:Maltose / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000378:metabolism; D009004:Monosaccharide Transport Proteins; D009154:Mutation; D012331:RNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D008320:Maltose; C021534:maltose permease; D000520:alpha-Glucosidases