title: Transcription of genes involved in the earliest steps of actinorhodin biosynthesis in Streptomyces coelicolor.
abstract: A 170bp long BamHI-Sau3A DNA fragment from the actIII-actI intergenic region of the actinorhodin (Act) biosynthetic gene cluster of Streptomyces coelicolor A3(2) contains two promoters directing transcription in a divergent manner. One of them, the actIII promoter, is responsible for the transcription of the actIII gene and the other controls transcription of the adjacent actI region in the opposite direction. Weak activity of the actIII promoter can be detected in Streptomyces lividans and Bacillus subtilis in the absence but not in the presence of glucose. Neither promoter seems to function in Escherichia coli.
authors.affiliation: Centro Nacional de Biotecnologia, Madrid, Spain.
authors.forename: V
authors.initials: V
authors.lastname: Parro
authors.forename: D A
authors.initials: DA
authors.lastname: Hopwood
authors.forename: F
authors.initials: F
authors.lastname: Malpartida
authors.forename: R P
authors.initials: RP
authors.lastname: Mellado
mesh_terms: D000880:Anthraquinones / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D000095622:Benzoisochromanequinones
chemical_list: D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; C013390:actinorhodin; D000095622:Benzoisochromanequinones