title: 13C-NMR: a simple yet comprehensive method for analysis of intermediary metabolism.
abstract: 13C-NMR is a particularly attractive tool for metabolic studies because of its inherent simplicity: all labeled products at sufficient concentration may be identified and analysed in a single spectrum. However, the real power behind the approach presented here is the ability to measure groups of individual 13C-isotopomers (isotope isomers). The information that this provides is superior to conventional tracer techniques, allowing a very detailed description of metabolic events. Several examples are given of the value of this convenient and straightforward analysis for some problems of current interest in intermediary metabolism.
authors.affiliation: Mary Nell and Ralph B. Rogers Magnetic Resonance Center, Department of Radiology, Dallas 75235-8573.
authors.forename: F M
authors.initials: FM
authors.lastname: Jeffrey
authors.forename: A
authors.initials: A
authors.lastname: Rajagopal
authors.forename: C R
authors.initials: CR
authors.lastname: Malloy
authors.forename: A D
authors.initials: AD
authors.lastname: Sherry
mesh_terms: D000818:Animals; D002247:Carbon Isotopes; D002952:Citric Acid Cycle; D003198:Computer Simulation; D005227:Fatty Acids / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009206:Myocardium / Q000378:metabolism; D051381:Rats
chemical_list: D002247:Carbon Isotopes; D005227:Fatty Acids