Beta-endorphin in pituitary intraglandular colloid of intermediate lobe origin.
Radioimmunoassay (RIA) detected the presence of beta-endorphin in the intraglandular colloid (IGC) of bovine pituitary intermediate lobe origin. The amount of beta-endorphin recovered in each of twelve samples ranged from 0.15 to 218.30 pmol/mg protein. A second group of assays [amino acid analysis, high performance liquid chromatography (HPLC) and mass spectral analysis] confirmed the RIA findings in another series of colloid samples. Approximately 75 pmol was collected from eight pooled glands. beta-Endorphin is an addition to the list of peptide hormones (e.g., methionine-enkephalin, adrenocorticotropin, arginine-vasopressin, alpha-melanocyte-stimulating hormone, beta-lipotropin and somatostatin) previously discovered in IGC by this laboratory.
Boyd
W H
WH
College of Biological Sciences, School of Human Biology, University of Guelph, Ontario, Canada.
Jichici
D
D
Agro
A
A
Rowell
G
G
Gibbs
B F
BF
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D008297:Male; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000737:chemistry*; D011863:Radioimmunoassay; D016339:Spectrometry, Mass, Fast Atom Bombardment; D001615:beta-Endorphin / Q000032:analysis*
D000596:Amino Acids; D001615:beta-Endorphin