Study of the interaction of cadmium with membrane-bound succinate dehydrogenase.
Cadmium ions inhibit membrane-bound succinate dehydrogenase with a second-order rate constant of 10.42 mM-1 s-1 at pH 7.35 and 25 degrees C. Succinate and malonate protect the enzyme against cadmium ion inhibition. The protection pattern exerted by succinate and malonate suggests that the group modified by cadmium is located at the active site. The pH curve of inactivation by Cd2+ indicates the involvement of an amino acid residue with pKa of 7.23.
Jay
D
D
Departamento de Bioquímica, Instituto Nacional de Cardiología, México, D.F., Mexico.
Zamorano
R
R
Muñoz
E
E
Gleason
R
R
Boldu
J L
JL
D000818:Animals; D001665:Binding Sites; D002104:Cadmium / Q000494:pharmacology*; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000201:enzymology; D007700:Kinetics; D008314:Malonates / Q000378:metabolism; D008929:Mitochondria, Heart / Q000201:enzymology; D013385:Succinate Dehydrogenase / Q000037:antagonists & inhibitors*; D013386:Succinates / Q000378:metabolism
D008314:Malonates; D013386:Succinates; D002104:Cadmium; C030290:malonic acid; D013385:Succinate Dehydrogenase