Growth and survival of Bordetella bronchiseptica in natural waters and in buffered saline without added nutrients.
Bordetella bronchiseptica showed increases in viable count when incubated in phosphate-buffered saline (PBS), in reagent-grade water, and in local lake and pond waters, all without added nutrients. Within 48 to 72 h at 37 degrees C in PBS and in lake and pond waters, stationary-phase populations of around 2.7 x 10(6) CFU/ml developed from washed B. bronchiseptica inocula of around 2 x 10(3) CFU/ml. Increases in CFU on the order of five- and eightfold, respectively, were observed in reagent-grade water and in seawater from the same sizes of inocula. The organisms remained viable for at least 3 weeks in PBS and in lake waters at 37 degrees C. The possibility that carry-over of nutrients was responsible for growth was discounted by showing serial transfer of B. bronchiseptica in PBS under conditions in which Escherichia coli tested in parallel rapidly died out.
Porter
J F
JF
Department of Microbiology, School of Biological Sciences, University of Glasgow, Scotland, United Kingdom.
Parton
R
R
Wardlaw
A C
AC
D001884:Bordetella / Q000254:growth & development* / Q000378:metabolism; D002021:Buffers; D003470:Culture Media; D003505:Cyclodextrins / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D012965:Sodium Chloride; D014867:Water
D002021:Buffers; D003470:Culture Media; D003505:Cyclodextrins; D010710:Phosphates; D014867:Water; D012965:Sodium Chloride