title: Media and methods for isolation of aeromonads from fecal specimens. A multilaboratory study.
abstract: An international multilaboratory study was conducted to establish the optimal combination of culture media, incubation time and temperature for recovery of aeromonads from stools using blood ampicillin (10 mg/l) agar (BAA), starch ampicillin (10 mg/l) agar (SAA), and cefsulodin irgasan novobiocin agar (CIN) with alkaline peptone water (APW) for enrichment. Optimal recovery of aeromonads (167/186) occurred using BAA (37 degrees C; 24 h) and CIN (25 degrees C; 48 h) with APW (25 degrees C; 24 h) subcultured to BAA (37 degrees C; 24 h) and CIN (25 degrees C; 48 h).
authors.affiliation: Hygienic Laboratory, University of Iowa City.
authors.forename: N P
authors.initials: NP
authors.lastname: Moyer
authors.forename: H K
authors.initials: HK
authors.lastname: Geiss
authors.forename: M
authors.initials: M
authors.lastname: Marinescu
authors.forename: A
authors.initials: A
authors.lastname: Rigby
authors.forename: J
authors.initials: J
authors.lastname: Robinson
authors.forename: M
authors.initials: M
authors.lastname: Altwegg
mesh_terms: D000333:Aeromonas / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D003470:Culture Media; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology; D006801:Humans; D007391:International Cooperation
chemical_list: D003470:Culture Media