Effect of adenosine and glucagon on hepatic blood volume responses to sympathetic nerves.
Hepatic blood volume responses were studied in cats using in vivo plethysmography. The maximal response (Rmax) to sympathetic nerve stimulation and to infusions of norepinephrine into the hepatic artery or portal vein was similar (12-14 mL expelled per liver in 2.9-kg cats; average liver weight, 76.8 +/- 6.8 g). The ED50 for norepinephrine intraportal (0.44 +/- 0.13) and intrahepatic arterial infusions (0.33 +/- 0.08 micrograms.kg-1.min-1) were similar indicating equal access of both blood supplies to the capacitance vessels. Adenosine (2.0 mg.kg-1.min-1) did not cause significant volume changes but produced a mild (27%) suppression of Rmax due to nerve stimulation with no change in the frequency (3.4 Hz) needed to produce 50% of Rmax. Rmax tended (not statistically significant) to decrease during glucagon (1.0 micrograms.kg-1.min-1) infusion but the nerve frequency needed to produce 50% of Rmax rose to 5.6 Hz. Thus both adenosine and glucagon produced modulation of sympathetic nerve-induced capacitance responses without having significant effects on basal blood volume. Adenosine, by virtue of its marked effects on arterial resistance vessels (at substantially lower doses than those used here) and the relative lack of effect on venous capacitance vessels, may be useful for producing clinical afterload reduction without venous pooling.
Lautt
W W
WW
Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
Schafer
J
J
Legare
D J
DJ
D000241:Adenosine / Q000494:pharmacology*; D000818:Animals; D002415:Cats; D004558:Electric Stimulation; D005934:Glucagon / Q000494:pharmacology*; D007262:Infusions, Intravenous; D007700:Kinetics; D008102:Liver Circulation / Q000187:drug effects / Q000502:physiology*; D009638:Norepinephrine / Q000008:administration & dosage / Q000494:pharmacology; D009929:Organ Size / Q000187:drug effects; D010991:Plethysmography; D012044:Regression Analysis; D013564:Sympathetic Nervous System / Q000187:drug effects / Q000502:physiology*
D005934:Glucagon; D000241:Adenosine; D009638:Norepinephrine