Tumor necrosis factor alpha selectively sensitizes human immunodeficiency virus-infected cells to heat and radiation.
We report here that infection of the human T-cell line HUT-78 with human immunodeficiency virus (HIV) increases its sensitivity to heat and radiation toxicity. A possible explanation for this result may be the reduced expression of manganous superoxide dismutase (MnSOD) in HIV-infected cells compared to uninfected cells. Tumor necrosis factor alpha (TNF-alpha) further sensitizes HIV-infected cells but not uninfected cells to heat and radiation. This is consistent with the ability of TNF-alpha to induce the expression of MnSOD in uninfected but not in HIV-infected cells. HIV-infected HUT-78 cell lines engineered to overexpress MnSOD are more resistant to heat and radiation than HIV-infected cells that do not overexpress MnSOD. However, treatment with TNF-alpha still sensitizes these cells to heat and radiation.
Wong
G H
GH
Department of Molecular Biology, Genentech, Inc., South San Francisco, CA 94080.
McHugh
T
T
Weber
R
R
Goeddel
D V
DV
D002460:Cell Line; D002470:Cell Survival; D015870:Gene Expression; D006678:HIV / Q000502:physiology*; D006358:Hot Temperature; D006801:Humans; D008345:Manganese; D012333:RNA, Messenger / Q000235:genetics; D013482:Superoxide Dismutase / Q000235:genetics; D013601:T-Lymphocytes / Q000201:enzymology / Q000382:microbiology* / Q000528:radiation effects; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D008345:Manganese; D013482:Superoxide Dismutase