Serotonin inhibition of tumor necrosis factor-alpha synthesis by human monocytes.
Serotonin inhibited in a concentration dependent way (10(-3) M to 10(-10) M) the LPS induced Tumor Necrosis Factor-alpha synthesis both, when added to the monocyte cultures from the beginning and when added together with the activating stimulus 8 hours before the end of the culture. The inhibitory effect was specifically blocked by the 5-HT1 and 5-HT2 serotonin antagonist methysergide and the 5-HT2 receptor antagonist ketanserin. This indicates that only the 5-HT2 receptor family (5-HT2 or 5-HT1C) may be involved in the inhibitory effect. Serotonin seems to play an important immunomodulatory role in macrophage functions.
Arzt
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E
Departamento de Sustancias Vasoactivas, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
Costas
M
M
Finkielman
S
S
Nahmod
V E
VE
D006801:Humans; D007650:Ketanserin / Q000494:pharmacology; D008784:Methysergide / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D011985:Receptors, Serotonin / Q000187:drug effects; D012701:Serotonin / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000096:biosynthesis
D011985:Receptors, Serotonin; D014409:Tumor Necrosis Factor-alpha; D012701:Serotonin; D007650:Ketanserin; D008784:Methysergide