title: Manganese peroxidase gene transcription in Phanerochaete chrysosporium: activation by manganese.
abstract: The expression of manganese peroxidase in nitrogen-limited cultures of Phanerochaete chrysosporium is dependent on Mn, and initial work suggested that Mn regulates transcription of the mnp gene. In this study, using Northern (RNA) blot analysis of kinetic, dose-response, and inhibitor experiments, we demonstrate unequivocally that Mn regulates mnp gene transcription. The amount of mnp mRNA in cells of 4-day-old nitrogen-limited cultures is a direct function of the concentration of Mn in the culture medium up to a maximum of 180 microM. Addition of Mn to nitrogen-limited Mn-deficient secondary metabolic (4-, 5-, and 6-day-old) cultures results in the appearance of mnp mRNA within 40 min. The appearance of this message is completely inhibited by the RNA synthesis inhibitor dactinomycin but not by the protein synthesis inhibitor cycloheximide. Furthermore, the amount of mnp mRNA produced is a direct function of the concentration of added Mn. In contrast, addition of Mn to low-nitrogen Mn-deficient 2- or 3-day-old cultures does not result in the appearance of mnp mRNA. Manganese peroxidase protein is detected by specific immunoprecipitation of the in vitro translation products of poly(A) RNA isolated from Mn-supplemented (but not from Mn-deficient) cells. All of these results demonstrate that Mn, the substrate for the enzyme, regulates mnp gene transcription via a growth-stage-specific and concentration-dependent mechanism.
authors.affiliation: Department of Chemical and Biological Sciences, Oregon Graduate Institute of Science and Technology, Beaverton 97006-1999.
authors.forename: J A
authors.initials: JA
authors.lastname: Brown
authors.forename: M
authors.initials: M
authors.lastname: Alic
authors.forename: M H
authors.initials: MH
authors.lastname: Gold
mesh_terms: D001483:Base Sequence; D001487:Basidiomycota / Q000201:enzymology / Q000235:genetics*; D004789:Enzyme Activation / Q000187:drug effects; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D008345:Manganese / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D008345:Manganese; D010544:Peroxidases; C051129:manganese peroxidase