Repression of the cell wall protein gene operon in Bacillus brevis 47 by magnesium and calcium ions.
Transcription from P2, one of the major promoters of the cell wall protein gene operon of Bacillus brevis 47, was markedly enhanced at the early stationary phase of growth. MgCl2, when added at 1 to 5 mM to the medium, inhibited this enhancement of transcription as well as shedding of the cell wall protein layers from the cell surface. MgSO4 or CaCl2 showed an effect similar to that of MgCl2. The possible coordination of the cell wall structure with regulation of the cell wall protein genes is discussed.
Adachi
T
T
Department of Food Science and Technology, Faculty of Agriculture, Nagoya University, Japan.
Yamagata
H
H
Tsukagoshi
N
N
Udaka
S
S
D001407:Bacillus / Q000187:drug effects / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D002122:Calcium Chloride / Q000494:pharmacology*; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism*; D015636:Magnesium Chloride / Q000494:pharmacology*; D009876:Operon; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013489:Suppression, Genetic; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D015636:Magnesium Chloride; D002122:Calcium Chloride