title: Obtention of a human primary humoral response against schistosome protective antigens in severe combined immunodeficiency mice after the transfer of human peripheral blood mononuclear cells.
abstract: Peripheral blood mononuclear cells (PBMC) from healthy donors were injected into C.B.-17 severe combined immunodeficiency (scid) mice which were subsequently immunized with crude Schistosoma mansoni adult worm antigenic preparation (SWAP) or with recombinant S. mansoni 28-kDa glutathione transferase (r-Sm-28-GST) antigen. PBMC from a S. mansoni-infected patient were also transferred. The specific human anti-SWAP and anti-Sm-28-GST antibody responses were monitored. The presence in both cases of human specific antibodies in scid mouse sera was determined by enzyme-linked immunosorbent assay and Western blotting techniques using anti-human immunoglobulin reagents. No antibodies were detected in these sera using anti-mouse immunoglobulin antisera. These antibodies were functional since a cytotoxic activity against schistosomula was observed when monocytes were incubated with scid mouse sera positive for anti-Sm-28-GST antibodies.
authors.affiliation: Centre d'Immunologie des maladies transmissibles et allergiques, Unité mixte INSERM 167-CNRS 624, Institut Pasteur, Lille, France.
authors.forename: C
authors.initials: C
authors.lastname: Mazingue
authors.forename: F
authors.initials: F
authors.lastname: Cottrez
authors.forename: C
authors.initials: C
authors.lastname: Auriault
authors.forename: J Y
authors.initials: JY
authors.lastname: Cesbron
authors.forename: A
authors.initials: A
authors.lastname: Capron
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D000947:Antigens, Helminth / Q000276:immunology*; D007153:Immunologic Deficiency Syndromes / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D051379:Mice; D012550:Schistosoma mansoni / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth