title: Abnormalities in the spontaneous firing patterns of cultured rat neocortical neurons after chronic exposure to picrotoxin during development in vitro.
abstract: We have used the GABA-A antagonist picrotoxin (PTX) to investigate whether chronic disinhibition, leading to intensified neuronal firing, would induce a specific pattern of physiological alterations in cultured rat neocortex cells. Overall mean spontaneous discharge rates were little affected by 1 microM PTX but firing occurred mainly as repetitive high-frequency bursts of action potentials. This "phasic" pattern contrasted with the irregular, quasi-random, firing usually seen in control units. Neurons tested in normal growth medium after prolonged exposure to 1 microM PTX showed weaker interspike interval dependencies (Markov value) than in controls, along with reduced regularity in the occurrence of bursts. Since all physiological changes were opposite in direction to those reported earlier after chronic suppression of bioelectric activity, the results support the hypothesis that endogenous synaptic and/or action potentials are important for the maturation of neocortical networks. Since experimental alterations were found only in spike-train parameters which reflect ontogenetic changes in untreated control cultures, GABAergic inhibition (by preventing neuronal discharges from becoming too intense) presumably serves to constrain the rate of development within optimal limits.
authors.affiliation: The Netherlands Institute for Brain Research, Amsterdam.
authors.forename: G J
authors.initials: GJ
authors.lastname: Ramakers
authors.forename: M A
authors.initials: MA
authors.lastname: Corner
authors.forename: A M
authors.initials: AM
authors.lastname: Habets
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000502:physiology*; D004247:DNA / Q000378:metabolism; D004594:Electrophysiology; D005260:Female; D005333:Fetus / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D010852:Picrotoxin / Q000633:toxicity*; D011247:Pregnancy; D051381:Rats
chemical_list: D004247:DNA; D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; D010751:Phosphopyruvate Hydratase; D010852:Picrotoxin