Release of interleukin-6 from anterior pituitary cell aggregates: developmental pattern and modulation by glucocorticoids and forskolin.
The release of the immunologically active cytokine interleukin-6 (IL-6) by cultured anterior pituitary cell aggregates was found to increase with the age of the donor rats. The glucocorticoid hormone dexamethasone (DEX) dose-dependently inhibited the release of IL-6, the IC50 being 2.43 +/- 0.93 nM. The sex steroids dihydrotestosterone and estradiol were without detectable effect. The adenylate cyclase activator forskolin increased IL-6 release in aggregate cultures in the presence of DEX. It is concluded that IL-6 release by anterior pituitary cells is subject to developmental changes and under regulatory control of biologically active substances.
Carmeliet
P
P
Laboratory of Cell Pharmacology, University of Leuven School of Medicine, Belgium.
Vankelecom
H
H
Van Damme
J
J
Billiau
A
A
Denef
C
C
D000262:Adenylyl Cyclases / Q000378:metabolism; D000375:Aging / Q000502:physiology*; D000818:Animals; D002449:Cell Aggregation; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology*; D013196:Dihydrotestosterone / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D015850:Interleukin-6 / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D015850:Interleukin-6; D013196:Dihydrotestosterone; D005576:Colforsin; D004958:Estradiol; D003907:Dexamethasone; D000262:Adenylyl Cyclases