title: Amplification immunoassay for the determination of hepatitis B surface antigen.
abstract: A sensitive sandwich immunoassay for the determination of Hepatitis B surface antigen (HBs) was developed, using a cascade system of Limulus amebocyte lysate as a signal amplification system. Lipopolysaccharide (LPS) was conjugated to anti-HBs antibody. Anti-HBs antibody was adsorbed to polystyrene beads. First, HBs were reacted to solid phase anti-HBs antibody (a-HBs). After the reaction, the beads were rinsed, and were then reacted with a-HBs-LPS. Then, LPS activity specifically bound to the beads was measured. HBs could be measured in the range of 10(-10)-10(-12) g/mL.
authors.affiliation: Research Center for Advanced Science and Technology, University of Tokyo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Seki
authors.forename: E
authors.initials: E
authors.lastname: Tamiya
authors.forename: I
authors.initials: I
authors.lastname: Karube
mesh_terms: D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D007118:Immunoassay / Q000379:methods*; D008070:Lipopolysaccharides; D008863:Microspheres; D011137:Polystyrenes
chemical_list: D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D008070:Lipopolysaccharides; D011137:Polystyrenes