title: Determination of gangliosides and sulfatide in human cerebrospinal fluid with a microimmunoaffinity technique.
abstract: An immunostaining procedure has been developed for the assay of the gangliotetraose gangliosides and sulfatide in cerebrospinal fluid. Gangliosides of the gangliotetraose series were individually determined with cholera toxin B-subunit (CT-B) and an anti CT-B monoclonal antibody after chromatography and sialidase hydrolysis to GM1 on high performance thin-layer plates. Sulfatide was determined by thin-layer chromatography using an anti-sulfatide antibody. The method was applied to normal cerebrospinal fluid from 20 adults and 30 children. The concentration of the gangliotetraose series gangliosides in adults varied from 100-300 nmol/l with a mean value of 230 +/- 56 nmol/l. Corresponding values for sulfatide were 30-225 nmol/l and 140 +/- 46 nmol/l. The values for gangliosides and sulfatide in children increased during development. The major gangliosides in cerebrospinal fluid of adults were GT1b and GD1b and in children GD1a and GT1b.
authors.affiliation: Department of Psychiatry and Neuroschemistry, University of Göteborg, St. Jörgen Hospital, Hisings Backa, Sweden.
authors.forename: P
authors.initials: P
authors.lastname: Davidsson
authors.forename: P
authors.initials: P
authors.lastname: Fredman
authors.forename: J E
authors.initials: JE
authors.lastname: Månsson
authors.forename: L
authors.initials: L
authors.lastname: Svennerholm
mesh_terms: D000293:Adolescent; D000368:Aged; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004797:Enzyme-Linked Immunosorbent Assay; D005677:G(M1) Ganglioside / Q000134:cerebrospinal fluid; D005732:Gangliosides / Q000134:cerebrospinal fluid*; D006801:Humans; D007223:Infant; D008875:Middle Aged; D012016:Reference Values; D013433:Sulfoglycosphingolipids / Q000134:cerebrospinal fluid*
chemical_list: D005732:Gangliosides; D013433:Sulfoglycosphingolipids; C021298:ganglioside, GD1a; C033851:ganglioside, GD1b; D005677:G(M1) Ganglioside; C016481:trisialoganglioside GT1