title: Marrow space star volume in the iliac crest decreases in osteoporotic patients after continuous treatment with fluoride, calcium, and vitamin D2 for five years.
abstract: Iliac crest biopsies in osteoporotic patients were obtained before and after five years of continuous treatment with sodium fluoride (40-60 mg/d), vitamin D2 (18,000 IE/d), and calcium phosphate (45 mmol/d). Star volume of marrow space and trabeculae, mean trabecular thickness, and fractional amount of trabecular bone were estimated on so-called vertical sections before and after treatment. Results showed a significant decrease in marrow space star volume after five years of treatment (35 mm3 versus 19 mm3) (2p less than 0.02). No significant changes were observed in trabecular star volume (0.149 mm3 versus 0.104 mm3) (2p greater than 0.50) or mean trabecular thickness (85 microns versus 79 microns, 2p greater than 0.30). A slight but insignificant increase in the fractional amount of trabecular bone was found (9.7% versus 10.4%, 2p greater than 0.50). A rather close inverse correlation was obtained, however, between individual changes in trabecular bone volume fraction and marrow space star volume (r = -0.72, 2p = 0.011). The findings suggest that the large marrow "cavities" had been split into two or more "cavities" by some newly generated trabecular structures leading to increased trabecular connectivity. The strength of trabecular bone may be increased due to this beneficial effect of fluoride on trabecular bone structure.
authors.affiliation: Aarhus Bone and Mineral Research Group, University Institute of Pathology, Aarhus Amtssygehus, Denmark.
authors.forename: A
authors.initials: A
authors.lastname: Vesterby
authors.forename: H J
authors.initials: HJ
authors.lastname: Gundersen
authors.forename: F
authors.initials: F
authors.lastname: Melsen
authors.forename: L
authors.initials: L
authors.lastname: Mosekilde
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D002118:Calcium / Q000627:therapeutic use*; D004359:Drug Therapy, Combination; D004872:Ergocalciferols / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D007085:Ilium / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D010024:Osteoporosis / Q000188:drug therapy* / Q000473:pathology; D012969:Sodium Fluoride / Q000627:therapeutic use*; D013997:Time Factors
chemical_list: D004872:Ergocalciferols; D012969:Sodium Fluoride; D002118:Calcium