The human multicatalytic proteinase: affinity purification using a monoclonal antibody.
A monoclonal antibody, coupled to Sepharose CL-4B, was used for the rapid purification of the human multicatalytic proteinase in a single chromatographic step under mild conditions. The enzyme was homogeneous as judged by nondenaturing polyacrylamide gel electrophoresis. Electrophoresis under dissociating and reducing conditions revealed at least ten components with molecular masses in the range 22-34 kDa. Affinity-purified enzyme was identical to conventionally purified enzyme with respect to enzymatic properties, molecular mass and subunit composition.
Hendil
K B
KB
August Krogh Institute, Copenhagen, Denmark.
Uerkvitz
W
W
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity / Q000379:methods*; D003546:Cysteine Endopeptidases / Q000276:immunology / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000276:immunology / Q000302:isolation & purification*; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D046988:Proteasome Endopeptidase Complex
D000911:Antibodies, Monoclonal; D009097:Multienzyme Complexes; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex