The effect of a high-fat diet on murine macrophage activity.
The phagocytic, oxygen free radical generating and cytotoxic activities of macrophages from C57BL/6J mice fed either a normal or an atherogenic high-fat diet have been investigated. Phagocytosis of aggregated low density lipoprotein (LDL) was only slightly inhibited by the high-fat diet although phorbol myristate acetate (PMA)-induced hydrogen peroxide (H2O2) and superoxide anion (O2-) production was significantly reduced. Activation of tumoricidal activity against L929 target cells by lipopolysaccharide (LPS) or lymphocyte-derived macrophage-activating factor (MAF), but not N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP), was also significantly reduced in macrophages from mice fed the high-fat diet. These results indicate that an atherogenic diet is capable of significantly affecting the responsiveness of macrophages to a number of stimulatory agents which act via specific membrane receptors.
Stewart-Phillips
J L
JL
Montreal General Hospital Research Institute, Quebec, Canada.
Lough
J
J
Phillips
N C
NC
D000818:Animals; D001161:Arteriosclerosis / Q000209:etiology; D003602:Cytotoxicity, Immunologic / Q000502:physiology; D004036:Diet, Atherogenic; D004041:Dietary Fats / Q000008:administration & dosage*; D005609:Free Radicals; D008262:Macrophage Activation / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010587:Phagocytosis / Q000502:physiology
D004041:Dietary Fats; D005609:Free Radicals