The antigenic structure of the human glycoprotein hormone alpha-subunit: III. Solution- and solid-phase mapping using synthetic peptides.
Twenty-seven synthetic peptides, representing the entire structure of the human glycoprotein hormone alpha-subunit were used to map the antigenic structure of the alpha-subunit. Solution phase and solid phase assays were performed with these peptides and a panel of eight monoclonal antibodies (MAb). Two dominant regions were localized between residues 22-37 and 70-87. All eight antibodies recognized these regions, but differed somewhat with respect to whether they saw the more N-terminal, middle, or C-terminal portions of these regions. The sequence of residues 13-22 was recognized by three MAbs. The C-terminal region from residues 84-92 was recognized by three MAbs. All MAbs recognized conformational epitopes in that they reacted with two or more regions. Three MAbs (two against free alpha and one against human CG) have linear amino acid sequences as part of their conformational epitope.
Charlesworth
M C
MC
Department of Biochemistry, Mayo Medical School, Rochester, Minnesota 55905.
Bergert
E R
ER
Morris
J C
JC
McCormick
D J
DJ
Ryan
R J
RJ
D000911:Antibodies, Monoclonal; D000941:Antigens / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000737:chemistry / Q000276:immunology*; D006728:Hormones / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D010449:Peptide Mapping / Q000379:methods*; D010455:Peptides / Q000138:chemical synthesis; D011863:Radioimmunoassay / Q000379:methods
D000911:Antibodies, Monoclonal; D000941:Antigens; D006023:Glycoproteins; D006728:Hormones; D010455:Peptides