[Criteria of the maturity of follicle and oocyte within the scope of in vitro fertilization. An overview].
The duration of preincubation of aspirated oocytes is essential for successful in vitro fertilization. The duration has to be varied according to the maturity of the oocyte and the follicle. The maturity of the oocyte corona-cumulus complex is assessed primarily by light microscopy. Additional information is provided by measurements of steroids, pituitary hormones, and proteins (such as inhibin, alpha 1-Antitrypsin, fibrinogen, plasminogen activator, collagenolytic enzymes, alpha 2-macroglobulin, fibronectin, glycosaminoglycans, insulin, insulin-like-growth-factors) in the follicular fluid. Complex measurements of so-called regulators of oocyte maturation (cAMP, oocyte maturation inhibitor, prostaglandins) is of high academic interest. Cytologic parameters, the chemotactic activity of the granulosa cells and serial ultrasound studies are of practical importance for evaluating the maturity of the growing follicle.
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Geburtshilflich-gynäkologischen Universitätsklinik, Graz.
D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D008853:Microscopy; D009865:Oocytes / Q000254:growth & development* / Q000648:ultrastructure; D006080:Ovarian Follicle / Q000033:anatomy & histology / Q000254:growth & development*; D010060:Ovulation / Q000502:physiology
Reifekriterien von Follikel und Eizelle im Rahmen der In-vitro-Fertilisierung. Eine Ubersicht.