Novel phosphonoglycosphingolipids containing pyruvylated galactose from the nervous system of Aplysia kurodai.
We have reported the existence of a phosphonoglycosphingolipid containing a pyruvylated galactose, FGL-IIb, in nerve fibers of Aplysia kurodai (Araki, S., Abe, S., Ando, S., Kon, K., Fujiwara, N. & Satake, M. (1989) J. Biol. Chem. 264, 19922-19927). We have now isolated two other pyruvylated galactose-containing phosphonoglycosphingolipids, named FGL-V and FGL-IIa, from the nervous tissue of Aplysia, and characterized them as [3,4-O-(S-1-carboxyethylidene)]Gal beta 1----3GalNAc alpha 1----3[6'-O-(2- aminoethylphosphonyl)Gal alpha 1----2] (2-aminoethylphosphonyl----6) Gal beta 1----4Glc beta 1----1 ceramide and [3,4,O-(S-1-carboxyethylidene)] Gal beta 1----3GalNAc alpha 1----3[6'-O-(2-aminoethylphosphonyl)Gal alpha 1----2]Gal beta 1----4Glc beta 11----ceramide, respectively. Their major aliphatic components are palmitic acid, octadeca-4-sphingenine and anteisononadeca-4-sphingenine. Thus, the nervous system of Aplysia contains several pyruvylated phosphonoglycolipids.
Araki
S
S
Department of Neurochemistry, Niigata University, Japan.
Abe
S
S
Satake
M
M
Hayashi
A
A
Kon
K
K
Ando
S
S
D000818:Animals; D001048:Aplysia; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005690:Galactose / Q000032:analysis*; D006028:Glycosphingolipids / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009420:Nervous System / Q000737:chemistry*; D063065:Organophosphonates / Q000302:isolation & purification*; D011773:Pyruvates / Q000032:analysis*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D006028:Glycosphingolipids; D063065:Organophosphonates; D011773:Pyruvates; D005690:Galactose