title: Time-resolved cryo-electron microscopic study of the dissociation of actomyosin induced by photolysis of photolabile nucleotides.
abstract: The rapid release of a substrate or other ligand from photolabile precursors in a thin layer suspension of biological specimens followed by rapid freezing provides a method of trapping and visualizing short-lived states in a dynamic system. We demonstrate here the first successful application of this method to study the interaction of actin filaments with myosin subfragment 1 (S1) after release of nucleotides. The results obtained suggest that structural changes in actin filaments occur as a result of interaction with S1.
authors.affiliation: Max Planck Institute for Medical Research, Department of Biophysics, Heidelberg, Federal Republic of Germany.
authors.forename: J F
authors.initials: JF
authors.lastname: Ménétret
authors.forename: W
authors.initials: W
authors.lastname: Hofmann
authors.forename: R R
authors.initials: RR
authors.lastname: Schröder
authors.forename: G
authors.initials: G
authors.lastname: Rapp
authors.forename: R S
authors.initials: RS
authors.lastname: Goody
mesh_terms: D000199:Actins / Q000648:ultrastructure; D000205:Actomyosin / Q000648:ultrastructure*; D000266:Adenylyl Imidodiphosphate / Q000378:metabolism / Q000494:pharmacology*; D005615:Freezing; D008854:Microscopy, Electron / Q000295:instrumentation / Q000379:methods; D010782:Photolysis
chemical_list: D000199:Actins; D000266:Adenylyl Imidodiphosphate; D000205:Actomyosin