Immunological detection of propolypeptide of von Willebrand factor on platelet surface.
Recently we have found that propolypeptide of von Willebrand factor (pp-vWF) obtained from platelets binds to type I collagen. It is known that pp-vWF is present in platelet alpha-granules and is secreted upon activation. In this paper, we demonstrate the two following evidences to show that it is also present on the surface of resting platelets. [1] The antibody against pp-vWF bound to the surface of platelets. [2] The antibody induced aggregation of platelets. The binding of the antibody and the antibody-induced aggregation of platelets were inhibited in a dose-dependent manner by Fab fragment of the antibody. Platelets from von Willebrand disease patients bound less of the antibody and responded weakly to the antibody.
Hashimoto
K
K
Department of Biological Sciences, Tokyo Institute of Technology, Japan.
Usui
T
T
Sasaki
K
K
Fujisawa
T
T
Sekiya
F
F
Takagi
J
J
Tsukada
T
T
Saito
Y
Y
D000906:Antibodies; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex; D001792:Blood Platelets / Q000737:chemistry / Q000502:physiology*; D002462:Cell Membrane / Q000737:chemistry / Q000502:physiology; D006801:Humans; D015151:Immunoblotting; D007700:Kinetics; D008970:Molecular Weight; D010974:Platelet Aggregation; D011498:Protein Precursors / Q000032:analysis* / Q000276:immunology / Q000502:physiology; D014841:von Willebrand Factor / Q000032:analysis* / Q000276:immunology / Q000502:physiology
D000906:Antibodies; D000936:Antigen-Antibody Complex; D011498:Protein Precursors; D014841:von Willebrand Factor; C067003:von Willebrand factor propolypeptide