Crystallization of the calcium-activated phosphoenolpyruvate carboxykinase from Escherichia coli K12.
Single crystals of phosphoenolpyruvate carboxykinase from Escherichia coli K12 have been grown in the orthorhombic crystal system. Single crystals developed to a maximum size of 0.25 mm x 0.25 mm x 1.5 mm by the technique of washing and reseeding. The space group is P2(1)2(1)2(1), with a = 77.24 A, b = 89.18 A, c = 93.24 A and Z = 4; there is one enzyme molecule per crystallographic asymmetric unit and the solvent content is estimated to be 59%. The crystals diffract to at least 2.8 A d spacings and decompose in the X-ray beam after approximately two days of exposure.
Delbaere
L T
LT
Department of Biochemistry, University of Saskatchewan, Saskatoon, Canada.
Vandonselaar
M
M
Glaeske
D
D
Jabs
C
C
Goldie
H
H
D002118:Calcium / Q000378:metabolism*; D003460:Crystallization; D004926:Escherichia coli / Q000201:enzymology*; D015394:Molecular Structure; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000737:chemistry* / Q000378:metabolism; D014961:X-Ray Diffraction
D010729:Phosphoenolpyruvate Carboxykinase (GTP); D002118:Calcium