title: Free bone graft reconstruction of irradiated facial tissue: experimental effects of basic fibroblast growth factor stimulation.
abstract: A study was undertaken to evaluate the potential utility of basic fibroblast growth factor in the induction of angiogenesis and osseous healing in bone previously exposed to high doses of irradiation. Thirty New Zealand rabbits were evaluated by introducing basic fibroblast growth factor into irradiated mandibular resection sites either prior to or simultaneous with reconstruction by corticocancellous autografts harvested from the ilium. The fate of the free bone grafts was then evaluated at 90 days postoperatively by microangiographic, histologic, and fluorochrome bone-labeling techniques. Sequestration, necrosis, and failure to heal to recipient osseous margins was observed both clinically and histologically in all nontreated irradiated graft sites as well as those receiving simultaneous angiogenic stimulation at the time of graft placement. No fluorescent activity was seen in these graft groups. In the recipient sites pretreated with basic fibroblast growth factor prior to placement of the graft, healing and reestablishment of mandibular contour occurred in nearly 50 percent of the animals. Active bone formation was evident at cortical margins adjacent to the recipient sites but was absent in the more central cancellous regions of the grafts.
authors.affiliation: Plastic Surgery Section, Indiana University Medical Center.
authors.forename: B L
authors.initials: BL
authors.lastname: Eppley
authors.forename: D T
authors.initials: DT
authors.lastname: Connolly
authors.forename: T
authors.initials: T
authors.lastname: Winkelmann
authors.forename: A M
authors.initials: AM
authors.lastname: Sadove
authors.forename: D
authors.initials: D
authors.lastname: Heuvelman
authors.forename: J
authors.initials: J
authors.lastname: Feder
mesh_terms: D000818:Animals; D016025:Bone Transplantation / Q000379:methods*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006085:Graft Survival / Q000187:drug effects; D008334:Mandible / Q000098:blood supply / Q000473:pathology / Q000528:radiation effects; D010025:Osteoradionecrosis / Q000473:pathology / Q000601:surgery*; D011817:Rabbits; D014945:Wound Healing / Q000187:drug effects
chemical_list: D016222:Fibroblast Growth Factor 2