Synergistic effect on blastogenesis in murine spleen cells of lipopolysaccharide, lipid A, and acid-degraded polysaccharide from Fusobacterium nucleatum.
Interchangeable combinations of Fusobacterium nucleatum Fev1 lipopolysaccharide (LPS) with its split products by acetic acid hydrolysis, i.e. lipid A (LA) and degraded polysaccharide (PS), amplified the blastogenic response in murine spleen cell cultures as measured by [3H]thymidine uptake. Athymic murine spleen cells precultured with LPS-Fev1 for 48 h (stage 1), washed twice and cultured together with fresh cells and either LA or PS for 72 h (stage 2) gave a synergistic response over that found in spleen cell cultures of thymic mice. Spleen cells pre-cultured with LA or PS and with fresh cells and LPS-Fev1 added to stage 2 cultures gave less significant amplification compared with precultures of LPS and either LA or PS together with fresh cells added to stage 2. Precultures with LA, PS or LPS-Fev1 and with pokeweed mitogen (PWM) and fresh cells added produced an additional increment of synergy which was most pronounced in spleen cell cultures of normal mice.
Sveen
K
K
Gade Institute, Department of Microbiology and Immunology, University of Bergen, Norway.
Hofstad
T
T
D000085:Acetates; D019342:Acetic Acid; D000818:Animals; D002478:Cells, Cultured; D004357:Drug Synergism; D005673:Fusobacterium / Q000276:immunology*; D006868:Hydrolysis; D008050:Lipid A / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008819:Mice, Nude; D011043:Pokeweed Mitogens / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000085:Acetates; C069667:FEV1 lipopolysaccharide; D008050:Lipid A; D008070:Lipopolysaccharides; D011043:Pokeweed Mitogens; D011135:Polysaccharides, Bacterial; D019342:Acetic Acid