A convenient method for differentiation of coagulase-negative staphylococci isolated from bovine mammary glands.
The utility of trehalose-mannitol broth and arabinose-cellobiose broth for identification of Staphylococcus epidermidis and novobiocin-resistant staphylococci was determined using 236 coagulase-negative staphylococci isolated from bovine mammary glands. None of the 49 S. epidermidis strains was positive in trehalose-mannitol broth; whereas, all strains of Staphylococcus hyicus, Staphylococcus chromogenes, Staphylococcus haemolyticus, Staphylococcus hominis, Staphylococcus warneri, and Staphylococcus simulans were positive. Of the novobiocin-resistant staphylococcal species, only Staphylococcus saprophyticus was negative in arabinose-cellobiose broth. Except for one strain of Staphylococcus sciuri and one strain of Staphylococcus kloosii, all remaining strains of novobiocin-resistant staphylococcal species were positive in arabinose-cellobiose broth. Results indicate that trehalose-mannitol broth is an acceptable method for identification of S. epidermidis isolated from bovine mammary glands. Furthermore, arabinose-cellobiose broth is a useful method of screening for novobiocin-resistant staphylococci.
Watts
J L
JL
Mastitis Research Laboratory, Louisiana State University Agricultural Center, Homer 71040.
Ray
C H
CH
Washburn
P J
PJ
D000818:Animals; D002417:Cattle / Q000382:microbiology*; D003030:Coagulase; D003470:Culture Media; D005260:Female; D008321:Mammary Glands, Animal / Q000382:microbiology*; D009675:Novobiocin / Q000494:pharmacology; D013210:Staphylococcus / Q000145:classification / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification*; D013212:Staphylococcus epidermidis / Q000145:classification / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification*
D003030:Coagulase; D003470:Culture Media; D009675:Novobiocin