title: Purification and amino acid sequence of vasoactive intestinal peptide, peptide histidine isoleucinamide and secretin from the ovine small intestine.
abstract: Vasoactive intestinal peptide (VIP), peptide histidine isoleucinamide (PHI) and secretin were separated and purified to homogeneity from ovine small intestine, using radioimmunoassay and radioreceptor assay for detection. An efficient and rapid purification sequence included acid extraction, concentration on a bulk C18 cartridge, filtration on a Fractogel column, ion-exchange chromatography on Mono-S and a maximum of three successive reverse-phase HPLC steps. The amounts of peptides obtained from 450 g wet weight tissue were 20 micrograms VIP, 15 micrograms PHI and 5 micrograms secretin. The as yet unknown amino acid sequences of the three peptides were found to be identical to those of the corresponding bovine peptides.
authors.affiliation: Department of Biochemistry and Nutrition, Medical School, Free University of Brussels, Belgium.
authors.forename: Y
authors.initials: Y
authors.lastname: Bounjoua
authors.forename: A
authors.initials: A
authors.lastname: Vandermeers
authors.forename: P
authors.initials: P
authors.lastname: Robberecht
authors.forename: M C
authors.initials: MC
authors.lastname: Vandermeers-Piret
authors.forename: J
authors.initials: J
authors.lastname: Christophe
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007421:Intestine, Small / Q000737:chemistry*; D008969:Molecular Sequence Data; D010451:Peptide PHI / Q000302:isolation & purification*; D012633:Secretin / Q000302:isolation & purification*; D012756:Sheep; D014660:Vasoactive Intestinal Peptide / Q000302:isolation & purification*
chemical_list: D010451:Peptide PHI; D012633:Secretin; D014660:Vasoactive Intestinal Peptide