title: Human enteric adenovirus type 41 binding to HEp-2 cells is specific.
abstract: Properties of human enteric adenovirus type 41 (Ad41) binding to its receptor on the surface of HEp-2 cells were investigated. The binding was found to be temperature-dependent, saturable, and specific. Analysis of the binding data showed a single class of 4.3 x 10(4) receptor sites per cell, an equilibrium dissociation constant of 21.0 nM, and no cooperativity among receptor sites. Trypsin-treated HEp-2 cells subsequently grown in the presence of tunicamycin or 2-deoxyglucose recovered full Ad41 binding activity, but could not if subsequently grown in the presence of cycloheximide. These data indicate that a single type of virus receptor, likely protein in nature, is present on the surface of HEp-2 cells to specifically bind Ad41.
authors.affiliation: Department of Microbiology, Immunology, Louisiana State University Medical Center, New Orleans 70112-1393.
authors.forename: H Y
authors.initials: HY
authors.lastname: Yeh
authors.forename: R B
authors.initials: RB
authors.lastname: Luftig
mesh_terms: D000260:Adenoviruses, Human / Q000187:drug effects / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D003847:Deoxyglucose / Q000494:pharmacology; D006801:Humans; D007822:Laryngeal Neoplasms; D011991:Receptors, Virus / Q000187:drug effects / Q000378:metabolism*; D014357:Trypsin / Q000494:pharmacology; D014415:Tunicamycin / Q000494:pharmacology
chemical_list: D011991:Receptors, Virus; D014415:Tunicamycin; D003513:Cycloheximide; D003847:Deoxyglucose; D014357:Trypsin