Detection of IgM antibodies against canine distemper virus in dog and mink sera employing enzyme-linked immunosorbent assay (ELISA).
An enzyme-linked immunosorbent assay (ELISA) for the detection of IgM antibodies against canine distemper virus (CDV) in canine and mink serum is described. The diagnostic potential of this technique was evaluated by analyzing sera from natural or experimental infections in dog and mink and negative control sera. These results were compared with results obtained in the developed CDV IgG ELISA and in the virus neutralization test. The IgM test, which requires only a single serum specimen, is a useful method for diagnosing current or recent CDV infections in dog and mink.
Blixenkrone-Møller
M
M
Department of Veterinary Microbiology, Royal Veterinary and Agricultural University of Copenhagen, Denmark.
Pedersen
I R
IR
Appel
M J
MJ
Griot
C
C
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D004216:Distemper / Q000175:diagnosis*; D004217:Distemper Virus, Canine / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D007106:Immune Sera / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis*; D008907:Mink; D009500:Neutralization Tests; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D014709:Vero Cells
D000914:Antibodies, Viral; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M