[A study of differential expression of HLA class II antigens in human lymphocytes].
Flow cytometric data of human lymphocyte subset in routine samples of patients have often shown differential expression of HLA Class II (Class II) antigens on T and B lymphocytes in our laboratory. I2 and I3 (both Coulter) were used as monoclonal antibodies for detection of Class II antigens. I2 was able to recognize HLA-DR except for haplotype of HLA-DR7, whose frequency was 1.8% in Japanese. Differential expression of Class II antigens of B lymphocytes in the present study was noticed in approximately 10% of our samples in frequency. In all of the 7 follow-up cases with renal transplantation, decrease of I2 expression on B lymphocytes was consistently noticed. On the other hand, increase of Class II expression on T lymphocytes was often recognized in autoimmune diseases, compared with those of diseases. Two of seven cases of hematologic neoplastic disorders were B cell type of malignant lymphoma and failed to express I2 despite the presence of I3 in terms of differential expression of Class II. In conclusion, the present study indicates that acquired decrease of HLA-DR expression on B lymphocytes was recognized in all of the studied patients with renal transplantation and that lack or decrease of HLA-DR expression on neoplastic B lymphocytes might be one of the characteristics of monoclonal growth.
Yamada
Y
Y
Department of Immunology, Toranomon Hospital, Tokyo.
Kanbayashi
H
H
Endo
Y
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001327:Autoimmune Diseases / Q000276:immunology; D002648:Child; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D016393:Lymphoma, B-Cell / Q000276:immunology; D008297:Male; D008875:Middle Aged
D000949:Histocompatibility Antigens Class II