title: Expression of type X collagen is transiently stimulated in redifferentiating chondrocytes pretreated with retinoic acid.
abstract: Growth of quail chondrocytes in the presence of retinoic acid (RA) results in the suppression of the differentiated phenotype. RA-treated chondrocytes recover their differentiated phenotype if they are cultured for an additional 15 days in the absence of RA. A few days after removal from RA, treated chondrocytes acquire the polygonal morphology characteristic of chondrocytes growing as attached cells; they also gradually resume collagen II expression and synthesize cultures. The levels of collagen X mRNA decrease during the second week of culture in the absence of RA. Finally, at the end of 15 days, the absolute levels of collagen II and collagen X mRNAs are very similar in control and recovering chondrocytes.
authors.affiliation: Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Sanchez
authors.forename: E
authors.initials: E
authors.lastname: Gionti
authors.forename: G
authors.initials: G
authors.lastname: Pontarelli
authors.forename: A
authors.initials: A
authors.lastname: Arcella
authors.forename: F
authors.initials: F
authors.lastname: De Lorenzo
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002356:Cartilage / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000235:genetics*; D015342:DNA Probes; D004625:Embryo, Nonmammalian; D015870:Gene Expression / Q000187:drug effects; D011784:Quail; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D015342:DNA Probes; D012333:RNA, Messenger; D014212:Tretinoin; D003094:Collagen