Superoxide dismutase enhances the toxicity of 3-hydroxyanthranilic acid to bacteria.
Cu,Zn.superoxide dismutase (SOD) enhanced the toxicity of 3-hydroxyanthranilic acid (3-HAT) to Salmonella typhimurium strain TA 102, evaluated as ability to form colonies. MnSOD showed the same effect. Inactivated Cu.ZnSOD had no effect. SODs accelerated the oxidation of 3-HAT, but inactivated Cu.ZnSOD caused little acceleration. It is proposed that the acceleration of 3-HAT oxidation leads to the enhancement of the 3-HAT toxicity. Catalase protected the bacteria from the toxicity of 3-HAT enhanced by Cu,ZnSOD, indicating that hydrogen peroxide generated in the oxidation of 3-HAT is involved in the toxicity. SODs accelerate the oxidation of 3-HAT and generate more hydrogen peroxide, that causes the enhancement of the 3-HAT toxicity to the bacteria. However, hydrogen peroxide alone was not so toxic. Hydrogen peroxide with 3-HAT was more toxic to the bacteria.
Ishii
T
T
Department of Biochemistry, Wakayama Medical College, Japan.
Iwahashi
H
H
Sugata
R
R
Kido
R
R
D015095:3-Hydroxyanthranilic Acid / Q000378:metabolism / Q000633:toxicity*; D002374:Catalase / Q000494:pharmacology; D004357:Drug Synergism; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000633:toxicity; D010084:Oxidation-Reduction; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000494:pharmacology*
D005609:Free Radicals; D015095:3-Hydroxyanthranilic Acid; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase