Differential presentation of hepatitis B S-preS(2) particles and peptides by macrophages and B-cell like antigen-presenting cells.
Different cell types, including dendritic cells, macrophages and Ia+ B cells, have been described to present soluble antigen (Ag) to T-cell hybridomas. However, it is still not clear whether these different cell types can act as antigen-presenting cells (APC) for complex and insoluble Ag such as viral particles. Using yeast recombinant hepatitis B S-preS(2)-containing particles, T-cell hybridomas were generated and used as a tool to study processing and presentation of antigen. Different types of APC were compared in regard to their capacity to process and present the protein-lipid composed S-preS(2) particles and the thereof derived T-cell epitope containing peptides by different types of APC. While a S-preS(2)-derived T-cell epitope containing peptide, which does not require processing, could be presented both by macrophage and B-cell like APC, the presentation of S-preS(2) particles required the presence of macrophages. The fact that B-cell like APC and macrophages behave differently with regard to the presentation of S-preS(2) particles suggest that the uptake and/or processing of this type of Ag by B-cell like APC and macrophages is different.
Scheerlinck
J P
JP
Institute of Molecular Biology, Vrije Universiteit Brussel, St-Geneius-Rode, Belgium.
Burssens
G
G
Brys
L
L
Michel
A
A
Hauser
P
P
De Baetselier
P
P
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D005260:Female; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006825:Hybridomas / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011498:Protein Precursors / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes
D000956:Antigens, Viral; D006514:Hepatitis B Surface Antigens; D000949:Histocompatibility Antigens Class II; D011498:Protein Precursors; C050807:presurface protein 2, hepatitis B surface antigen