HIV isolation from pulmonary cells derived from bronchoalveolar lavage.
In a study of 75 alveolar cell co-cultures from 55 HIV-seropositive subjects, p24 antigen production was identified by ELISA in 11 out of 26 (42%) of unseparated cell cultures, 15 out of 39 (38%) purified alveolar macrophage cultures and eight out of 10 purified alveolar lymphocyte samples. Positivity in unseparated cell cultures was associated with an alveolar lymphocytosis greater than 30%. Negative macrophage cultures were significantly more likely in the early stages of HIV infection with none positive when the subject had a peripheral CD4+ cell count greater than 300/microliters. Alveolar macrophage infection thus appears to increase with HIV disease progression.
Jeffrey
A A
AA
Hôpital Laennec, Paris, France.
Israël-Biet
D
D
Andrieu
J M
JM
Even
P
P
Venet
A
A
D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology*; D002478:Cells, Cultured; D006678:HIV / Q000302:isolation & purification*; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology; D008264:Macrophages / Q000382:microbiology; D011650:Pulmonary Alveoli / Q000382:microbiology