title: Nucleotide sequence of the replication region of the marine Rhodobacter plasmid pRD31.
abstract: The minimum region required for replication of the marine Rhodobacter endogenous plasmid pRD31 has been sequenced. This region is located on a 1367-bp HincII-PstI restriction fragment and is 62% rich in G-C base pairs. A region homologous to the bacteriophage P1 repA promoter, which overlaps two inverted repeats, has been identified. Plasmids with mutations in this 1367-bp region could not replicate in marine Rhodobacter hosts. This is the first identified replication origin of a photosynthetic bacterium.
authors.affiliation: Department of Biotechnology, Tokyo University of Agriculture and Technology, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Matsunaga
authors.forename: H
authors.initials: H
authors.lastname: Mihashita
authors.forename: M
authors.initials: M
authors.lastname: Miyake
authors.forename: J G
authors.initials: JG
authors.lastname: Burgess
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004261:DNA Replication; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012241:Rhodopseudomonas / Q000235:genetics*; D012623:Seawater