Isolation and characterization of authentic Phe-Met-Arg-Phe-NH2 and the novel Phe-Thr-Arg-Phe-NH2 peptide from Nereis diversicolor.
Immunocytochemical studies have shown that peptides like Phe-Met-Arg-Phe-NH2 (FMRFamide) are widely distributed throughout the nervous system of three Nereidae. In Nereis diversicolor we have isolated these peptides from an extract of total worms by affinity chromatography and two steps of reversed-phase high-performance liquid chromatography. The sequences of the purified peptides have been determined by amino acid sequencing and on the basis of their reactivity with an anti-FMRFamide serum specific for the determinant Arg-Phe-NH2. Two primary structures have been established: Phe-Thr-Arg-Phe-NH2 (FTRFamide) and PHe-Met-Arg-Phe-NH2 (FMRFamide). Furthermore a methionine sulfoxide derivative of the FMRFamide has been identified. We have synthesized the FTRFamide peptide and in all cases, the native peptides were indistinguishable from the synthetic counterparts. The structure of the two native peptides and of the methionyl sulfoxide derivate have been confirmed by fast-atom-bombardment and tandem mass spectrometry.
Baratte
B
B
Laboratoire d'Endocrinologie des Invertébrés, URA CNRS 148, Université de Lille Flandres-Artois, France.
Gras-Masse
H
H
Ricart
G
G
Bulet
P
P
Dhainaut-Courtois
N
N
D000595:Amino Acid Sequence; D000818:Animals; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D019835:FMRFamide; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification*; D011077:Polychaeta / Q000032:analysis*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D009479:Neuropeptides; C069392:phenylalanyl-threonyl-arginyl-phenylalaninamide; D019835:FMRFamide