title: Protein separation and purification in neat dimethyl sulfoxide.
abstract: Pure DMSO (instead of water) is used as the reaction medium for protein separations. It is shown that common extracellular proteins (i) have high solubility in DMSO (1-50 mg/ml), (ii) do not irreversibly inactivate in this solvent, and (iii) can adsorb onto carboxymethyl cellulose in DMSO and be subsequently fully desorbed in this solvent by inorganic salts. Ion-exchange chromatography on this resin in DMSO has been used to purify bovine pancreatic trypsin and to separate it from hen egg-white lysozyme in their mixture. Another approach to protein separation in DMSO, fractional precipitation with ethyl acetate (which does not dissolve proteins), has been verified with a mixture of bovine pancreatic chymotrypsinogen and chicken egg ovalbumin.
authors.affiliation: Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139.
authors.forename: N
authors.initials: N
authors.lastname: Chang
authors.forename: S J
authors.initials: SJ
authors.lastname: Hen
authors.forename: A M
authors.initials: AM
authors.lastname: Klibanov
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange / Q000379:methods; D004121:Dimethyl Sulfoxide; D004798:Enzymes / Q000302:isolation & purification* / Q000378:metabolism; D007202:Indicators and Reagents; D007700:Kinetics; D009113:Muramidase / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*; D012995:Solubility; D014357:Trypsin / Q000302:isolation & purification
chemical_list: D004798:Enzymes; D007202:Indicators and Reagents; D011506:Proteins; D009113:Muramidase; D014357:Trypsin; D004121:Dimethyl Sulfoxide