Ethacrynic acid-induced glutamate release from mouse brain synaptosomes.
A loop diuretic, ethacrynic acid (0.3 mM), released glutamate from mouse brain synaptosomes as potently as 40 mM K+, and was more potent than furosemide and bumetanide. Ethacrynic acid-induced glutamate release was suppressed by depletion of Ca2+ or Cl- from the incubation medium. The findings suggest that ethacrynic acid enhances glutamate release through Cl(-)-related depolarization of nerve endings.
Inoue
M
M
Department of Pharmacology, Kansai Medical University, Osaka, Japan.
Hirose
T
T
Inagaki
C
C
D015761:4-Aminopyridine / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000502:physiology*; D002118:Calcium / Q000494:pharmacology; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D004976:Ethacrynic Acid / Q000494:pharmacology*; D005971:Glutamates / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010042:Ouabain / Q000494:pharmacology; D013574:Synaptosomes / Q000187:drug effects / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology
D005971:Glutamates; D013779:Tetrodotoxin; D004024:Dicyclohexylcarbodiimide; D010042:Ouabain; D015761:4-Aminopyridine; D004976:Ethacrynic Acid; D002118:Calcium