title: Insulin stimulates fatty acid acylation of adipocyte proteins.
abstract: Isolated rat adipocytes were incubated with [3H]palmitate in the presence or absence of insulin. Insulin caused marked (20-fold), rapid (T1/2 approximately 15 min), and concentration-dependent labeling of an 80 kilodalton protein. The insulin concentration producing half-maximal labeling was 0.4 ng/ml. The label was removed from the protein by treatment with hydroxylamine indicating the fatty acid was linked to the protein via an oxyester bond. Label removed from the protein co-eluted with palmitate standard by high-performance liquid chromatography. In summary, adipocyte proteins are subject to acylation by exogenous long-chain fatty acids and this process is stimulated by insulin.
authors.affiliation: Department of Medicine, Medical College of Wisconsin, Zablocki Veterans Administration Hospital, Milwaukee 53295.
authors.forename: A
authors.initials: A
authors.lastname: Jochen
authors.forename: J
authors.initials: J
authors.lastname: Hays
authors.forename: E
authors.initials: E
authors.lastname: Lianos
authors.forename: S
authors.initials: S
authors.lastname: Hager
mesh_terms: D000215:Acylation; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000378:metabolism*; D019811:Hydroxylamine; D006898:Hydroxylamines / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D010168:Palmitates / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D005227:Fatty Acids; D006898:Hydroxylamines; D007328:Insulin; D010168:Palmitates; D011506:Proteins; D019811:Hydroxylamine