[The effect of intraperitoneal administration of adrenaline on ascorbic acid levels in the brain, adrenal glands and blood in rats of various ages].
Male and female rats (Wistar strain), 5 days old and full grown rats (cca90-120 days) were used for experiments where the authors investigated the effect of adrenaline SPOFA, 0.15 mg/kg body weight administered by the i.p. route, on the ascorbic acid content in the cerebral cortex, in the mesencephalon, cerebellum and medulla oblongata, as well as in the adrenals and plasma. Ascorbic acid was assessed by Roe and Kuether's method (19). The control group was formed by intact rats or a group where saline in analogous amounts (volume) was administered by the i.p. route. Tissues and plasma were processed 30 min. after administration of adrenaline (saline). The method of processing and collection of tissue specimens was described repeatedly in the past (10, 16). It was revealed that 5-day old rats regardless of sex have after administration of saline or adrenaline a higher ascorbic acid concentration in the brain (more frequently significant changes were found in males-incl. plasma). The same applies to the group of full grown males. In full grown females there is usually a decline of the ascorbic acid concentration in the brain (injection and administration of saline is more effective than adrenaline). In full grown rats concurrently a marked rise of the plasma concentration of ascorbic acid occurs.
Mourek
J
J
Fyziologický ústav 1. lékarské fakulty Univerzity Karlovy v Praze.
Janda
P
P
Koudelová
J
J
D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D001205:Ascorbic Acid / Q000097:blood / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D004837:Epinephrine / Q000008:administration & dosage* / Q000494:pharmacology; D005260:Female; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D001205:Ascorbic Acid; D004837:Epinephrine
Vliv intraperitoneálnĕ aplikovaného adrenalinu na obsah kyseliny askorbové v mozku, nadledvinĕ a v plazmĕ různĕ starých krys.