The complete amino acid sequence of bovine cathepsin S and a partial sequence of bovine cathepsin L.
The complete amino acid sequence of bovine spleen cathepsin S has been determined. The single-chain protein contains 217 residues and has a Mr of 23,682. The primary structure was determined by sequencing of native protein and the peptides obtained by proteolytic cleavage with beta-trypsin, papaya proteinase IV and by chemical cleavage with cyanogen bromide. Comparison of the amino terminal sequences of the heavy and the light chain of bovine cathepsin L with that of bovine cathepsin S clearly indicates that the enzymes are structurally different.
Ritonja
A
A
Department of Biochemistry, Jozef Stefan Institute, Ljubljana, Yugoslavia.
Colić
A
A
Dolenc
I
I
Ogrinc
T
T
Podobnik
M
M
Turk
V
V
D000595:Amino Acid Sequence; D000818:Animals; D056668:Cathepsin L; D002403:Cathepsins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D002417:Cattle; D003546:Cysteine Endopeptidases; D010450:Endopeptidases; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D013154:Spleen / Q000201:enzymology
D010446:Peptide Fragments; D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; C534281:CTSL protein, human; D056668:Cathepsin L; C028217:cathepsin S