A study of nephrotoxin-induced acute tubular necrosis with 31P magnetic resonance spectroscopy.
Phosphorus magnetic resonance spectroscopy (31P MRS) was used to obtain in vivo spectra from rat kidneys undergoing acute tubular necrosis induced by a nephrotoxic dose of cephaloridine (CLD). Spectra were obtained 0, 24, and 48 h after injection of CLD (experimental group, n = 6) or saline vehicle (control group, n = 6). The nephrotoxicity of CLD was demonstrated by severely increased serum creatinine levels and the development of extensive proximal tubular necrosis in the CLD-injected rats, and the lack of such changes in the controls. 31P MRS showed an increase in the inorganic phosphate region signal (Pi, p = 0.004) and a decrease in the phosphodiester region signal (PDE, p = 0.01) in the experimental group by 48 h, whereas these parameters did not vary significantly in the control group during the experiment. Significant correlations were found between serum creatinine and the same two 31P MRS parameters. In summary, rat kidneys which have developed severe CLD-induced proximal tubular necrosis exhibit changes in the 31P spectrum 48 h after administration of the drug. The causes of these changes were not determined.
Schutz
T
T
Department of Pediatrics, Stanford University School of Medicine, California 94305.
González-Méndez
R
R
Nabseth
D C
DC
Tune
B M
BM
Jardetzky
O
O
Stevenson
D K
DK
D000818:Animals; D002509:Cephaloridine / Q000009:adverse effects; D007668:Kidney / Q000378:metabolism / Q000473:pathology*; D007683:Kidney Tubular Necrosis, Acute / Q000139:chemically induced / Q000175:diagnosis*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D002509:Cephaloridine