title: Cholecystokinin mRNA detection in rat spinal cord motoneurons but not in dorsal root ganglia neurons.
abstract: Cholecystokinin (CCK) mRNA has been detected by in situ hybridization histochemistry using two different oligonucleotide probes in small to medium-sized neurons of layers II-III and X of Rexed and in large neurons of layer IX in the rat spinal cord at cervical, thoracic and lumbo-sacral levels. No labeled cells were detected in the dorsal root ganglia. This confirms the previously established distribution of CCK-like immunoreactivity in layers II-III and X and indicates, in addition, that motoneurons of layer IX may express true genuine CCK whilst conversely, dorsal root ganglia neurons do not.
authors.affiliation: Laboratory of Neuropathology and Neuropeptide Research, Brugmann Hospital, Université Libre de Bruxelles, Brussels.
authors.forename: S N
authors.initials: SN
authors.lastname: Schiffmann
authors.forename: E
authors.initials: E
authors.lastname: Teugels
authors.forename: P
authors.initials: P
authors.lastname: Halleux
authors.forename: R
authors.initials: R
authors.lastname: Menu
authors.forename: J J
authors.initials: JJ
authors.lastname: Vanderhaeghen
mesh_terms: D000818:Animals; D001345:Autoradiography; D002766:Cholecystokinin / Q000235:genetics*; D005727:Ganglia, Spinal / Q000166:cytology / Q000502:physiology*; D008297:Male; D009046:Motor Neurons / Q000166:cytology / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000166:cytology / Q000502:physiology*; D013462:Sulfur Radioisotopes
chemical_list: D012333:RNA, Messenger; D013462:Sulfur Radioisotopes; D002766:Cholecystokinin