An ultrastructural and biochemical analysis of the epithelial-mesenchymal interface of the ruminal mucosa during development.
The ultrastructure of fetal and adult bovine epithelial-mesenchymal interface of the ruminal mucosa was described and then correlated to the biochemical analysis for glycosaminoglycans (GAGs). During development a progressive undulation of the basement membrane, increase of connective tissue filamentous material, and folding of the cellular membrane of the basal epithelial cells were observed. Coincidently, a decrease in the relative composition of hyaluronic acid and an increase in chondroitin sulfate was observed. These results suggest a correlation between glycosaminoglycans composition and the expression of some cellular events resulting in morphological changes which operate during ruminal morphogenesis.
Arias
J L
JL
Department of Animal Biological Sciences, Faculty of Veterinary Sciences, University of Chile.
Fernandez
M S
MS
Oreste
P
P
Baeza
S
S
Pavez
V
V
D000818:Animals; D001485:Basement Membrane / Q000648:ultrastructure; D002417:Cattle / Q000033:anatomy & histology* / Q000196:embryology / Q000254:growth & development; D003599:Cytoskeleton / Q000648:ultrastructure; D003720:Densitometry; D004588:Electrophoresis, Cellulose Acetate; D004848:Epithelium / Q000737:chemistry / Q000196:embryology / Q000254:growth & development / Q000648:ultrastructure; D005753:Gastric Mucosa / Q000737:chemistry / Q000196:embryology / Q000254:growth & development / Q000648:ultrastructure; D006025:Glycosaminoglycans / Q000032:analysis*; D008854:Microscopy, Electron; D012417:Rumen / Q000737:chemistry / Q000196:embryology / Q000254:growth & development / Q000648:ultrastructure*
D006025:Glycosaminoglycans