Liquid chromatographic analysis of a potential polymeric-pendant drug delivery system for peptides. Application of high-performance size-exclusion chromatography, reversed-phase high-performance liquid chromatography and ion chromatography to the evaluation of biodegradable poly[(chloromethoxytrialanine methyl ester)phosphazenes].
A novel water-soluble polymer, poly[(chloromethoxytrialanine methyl ester)phosphazene] (poly-Tame), was characterized and evaluated using high-performance size-exclusion chromatography, gradient reversed-phase high-performance liquid chromatography and ion chromatography. These novel liquid chromatographic methods were validated for application to in vitro biodegradation experiments of poly-Tame in aqueous solutions. Results from method validation experiments are presented.
Eickhoff
W M
WM
Department of Drug Delivery, Sterling Research Group, Great Valley, PA 19355.
Liversidge
G G
GG
Mutharasan
R
R
D001673:Biodegradation, Environmental; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004337:Drug Carriers; D006863:Hydrogen-Ion Concentration; D007477:Ions; D009943:Organophosphorus Compounds / Q000032:analysis*; D010455:Peptides / Q000008:administration & dosage*; D011108:Polymers / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D004337:Drug Carriers; D007477:Ions; D009943:Organophosphorus Compounds; D010455:Peptides; D011108:Polymers; C069370:poly(chloromethoxytrialanine methyl ester)phosphazene