A predictive and feedback control algorithm maintains a constant glucose concentration in fed-batch fermentations.
A combined predictive and feedback control algorithm based on measurements of the concentration of glucose on-line has been developed to control fed-batch fermentations of Escherichia coli. The predictive control algorithm was based on the on-line calculation of glucose demand by the culture and plotting a linear regression to the next datum point to obtain a predicted glucose demand. This provided a predictive "coarse" control for the glucose-based nutrient feed. A direct feedback control using a proportional controller, based on glucose measurements every 2 min, fine-tuned the feed rate. These combined control schemes were used to maintain glucose concentrations in fed-batch fermentations as tight as 0.49 +/- 0.04 g/liter during growth of E. coli to high cell densities.
Kleman
G L
GL
Department of Microbiology, Ohio State University, Columbus 43210.
Chalmers
J J
JJ
Luli
G W
GW
Strohl
W R
WR
D000085:Acetates / Q000378:metabolism; D000465:Algorithms; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D005246:Feedback; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D012044:Regression Analysis
D000085:Acetates; D005947:Glucose