Evaluation of the antimicrobial activity of methylglyoxal bis(guanylhydrazone) analogues, the inhibitors for polyamine biosynthetic pathway.
Metabolic and antiproliferative effects of methylglyoxal bis(butylamidinohydrazone) (MGBB) and methylglyoxal bis(cyclopentylamidinohydrazone) (MGBCP), inhibitors for polyamine biosynthetic pathway, on Escherichia coli, Shigella sonnei, Aeromonas sobria, Aeromonas hydrophila and Vibrio cholerae were investigated. MGBB at the concentration of 100 mumol/l depleted intracellular putrescine and spermidine concentrations of E. coli to 25 and 20% of the controls, respectively, while MGBCP depressed their concentrations to 38 and 24%, respectively. In these polyamine-depleted E. coli cells the syntheses of RNA, DNA and protein decreased to 13, 54 and 29% of the control, respectively, with MGBB and to 23, 71 and 55%, respectively, with MGBCP. The minimum inhibitory concentrations (MIC) of MGBB for the growth of A. sobria, E. coli, A. hydrophila, V. cholerae and Sh. sonnei were estimated to be 50, 160, 240, 285 and 320 mumol/l, respectively, whereas those of MGBCP were slightly higher for respective bacteria.
Midorikawa
Y
Y
Department of Public Health, Mie University School of Medicine, Japan.
Hibasami
H
H
Gasaluck
P
P
Yoshimura
H
H
Masuji
A
A
Nakashima
K
K
Imai
M
M
D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development; D001426:Bacterial Proteins / Q000096:biosynthesis; D015317:Biogenic Polyamines / Q000096:biosynthesis*; D004926:Escherichia coli / Q000378:metabolism; D008935:Mitoguazone / Q000031:analogs & derivatives* / Q000494:pharmacology
D001426:Bacterial Proteins; D015317:Biogenic Polyamines; C059234:methylglyoxal bis(cyclopentylamidinohydrazone); C047167:methylglyoxal bis(butylamidinohydrazone); D008935:Mitoguazone