Phosphorylation of coagulation factor II by phospholipid/Ca(2+)-dependent protein kinase (protein kinase C).
Prothrombin is a major constituent of the blood coagulation cascade and requires phospholipid and Ca2+ for its activation. We have found that phospholipid/Ca(2+)-dependent protein kinase (Protein kinase C) phosphorylates prothrombin and the associated apparent Km value for prothrombin (0.86 microM) is comparable to the Km value reported for most known substrates of protein kinase C. A 2-dimension separation analysis revealed that serine residue was apparently phosphorylated by PKC. The phosphorylation was inhibited by such phosphatidylserine- and/or Ca2+ competitive protein kinase C inhibitors as trifluoperazine, palmitoylcarnitine and gossypol. These results suggest that protein kinase C phosphorylation was involved in the regulation of blood coagulation.
Abe
K
K
Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
Sakurada
K
K
Tanaka
M
M
Uehara
Y
Y
Matsuno
K
K
Miyazaki
T
T
Katoh
N
N
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D006072:Gossypol / Q000494:pharmacology; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology; D007700:Kinetics; D010172:Palmitoylcarnitine / Q000494:pharmacology; D010766:Phosphorylation; D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000302:isolation & purification / Q000378:metabolism*; D011516:Prothrombin / Q000302:isolation & purification / Q000378:metabolism*; D013154:Spleen / Q000201:enzymology; D013552:Swine; D013917:Thrombin / Q000302:isolation & purification / Q000378:metabolism; D014268:Trifluoperazine / Q000494:pharmacology
D000596:Amino Acids; D007546:Isoquinolines; D010879:Piperazines; D010172:Palmitoylcarnitine; D014268:Trifluoperazine; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011516:Prothrombin; D011493:Protein Kinase C; D013917:Thrombin; D006072:Gossypol