High-performance liquid chromatographic analysis of the unusual pathway of oxidation of L-arginine to citrulline and nitric oxide in mammalian cells.
A very unusual pathway of the oxidation of L-arginine to citrulline and nitric oxide has been discovered recently in cytotoxic macrophages. In an attempt to detect molecules generated through this metabolic pathway, a fast radio high-performance liquid chromatographic method was developed to analyse the whole set of radiolabelled L-arginine-derived metabolites produced by mammalian cells after appropriate induction. A new intermediate which might be NG-hydroxy-L-arginine was found.
Chenais
B
B
CNRS URA 1116, Université Paris-Sud, Orsay, France.
Yapo
A
A
Lepoivre
M
M
Tenu
J P
JP
D000230:Adenocarcinoma / Q000737:chemistry / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D001120:Arginine / Q000032:analysis / Q000378:metabolism*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002956:Citrulline / Q000378:metabolism*; D008325:Mammary Neoplasms, Experimental / Q000737:chemistry / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism*; D010084:Oxidation-Reduction
D002956:Citrulline; D009569:Nitric Oxide; D001120:Arginine