Isolation of a variant family of mouse minor satellite DNA that hybridizes preferentially to chromosome 4.
Two cosmids (HRS-1 and HRS-2) containing mouse minor satellite DNA sequences have been isolated from a mouse genomic library. In situ hybridization under moderate stringency conditions to metaphase chromosomes from RCS-5, a tumor cell line derived from the SJL strain, mapped both HRS-1 and HRS-2 to the centromeric region of chromosome 4. Sequence data indicate that these cloned minor satellite DNA sequences have a basic higher order repeat of 180 bp, composed of three diverged 60-bp monomers. Digestion of mouse genomic DNA with several restriction enzymes produces a ladder of minor satellite fragments based on a 120-bp repeat. The restriction enzyme NlaIII (CATG) digests all the minor satellite DNA into three prominent bands of 120, 240, and 360 bp and a weak band of 180 bp. Thus, the majority of minor satellite sequences in the genome are arranged in repeats based on a 120-bp dimer, while the family of minor satellite sequences described here represents a rare variant of these sequences. Our results raise the possibility that there may be other variant families of minor satellites analogous to those of alphoid DNA present in humans.
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Department of Molecular Biology and Genetics, Wayne State University, Detroit, Michigan 48201.
Trevor
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Miller
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Miller
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D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002503:Centromere; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003360:Cosmids; D004276:DNA, Satellite / Q000235:genetics*; D015723:Gene Library; D014644:Genetic Variation; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D014407:Tumor Cells, Cultured
D004276:DNA, Satellite