Effect of diamide on nucleoside and glucose transport in Plasmodium falciparum and Babesia bovis infected erythrocytes.
Normal human erythrocytes, preincubated with the oxidizing agent diamide, did not demonstrate any increased permeability, but showed a significant decrease in their ability to transport the nucleoside adenosine. Diamide appeared to have little effect on glucose permeation in uninfected and Plasmodium falciparum infected cells. The inhibition of adenosine transport in human erythrocytes by diamide pretreatment appeared to be unrelated to the inhibition by the established nucleoside transport inhibitor, nitrobenzylthioinosine (NBMPR). An ID50 for diamide of 0.3 mM was determined for 1 microM adenosine transport in human erythrocytes after preincubation for 45 min at 37 degrees C. However, preincubation of diamide (20 mM, 60 min at 37 degrees C) with Babesia bovis-infected bovine erythrocytes resulted in complete inhibition of the capacity of the parasitised cell to transport adenosine and partial inhibition of glucose permeation. By contrast, diamide was shown to have little or no effect on the new or induced nucleoside permeation site in P. falciparum (trophozoite) infected erythrocytes nor on the glucose transporter in these cells. The results further indicate the differences between the normal human erythrocyte nucleoside and glucose transporters and those new or altered transporters in the membrane of P. falciparum or B. bovis-infected red blood cells.
Gero
A M
AM
School of Biochemistry, University of New South Wales, Kensington, Australia.
Wood
A M
AM
Hogue
D L
DL
Upston
J M
JM
D000818:Animals; D001403:Babesia / Q000378:metabolism*; D001404:Babesiosis / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D002463:Cell Membrane Permeability; D003958:Diamide / Q000494:pharmacology*; D004912:Erythrocytes / Q000378:metabolism* / Q000469:parasitology; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008288:Malaria / Q000378:metabolism; D009705:Nucleosides / Q000378:metabolism*
D009705:Nucleosides; D003958:Diamide; D005947:Glucose