Purification on poly(U)-sepharose 4B of human breast cancer cell line T-47D DNA polymerases.
The purification of DNA polymerases (RNA-directed DNA polymerases and DNA-directed DNA polymerases) on poly(U)-Sepharose 4B from a breast tumour cell line (T-47D) is reported. The elution of these enzymes was followed in each fraction by activity measurements with the four primer-templates poly(rA)-oligo(dT)12-18, poly(dA) oligo(dT)12-18, poly(rC)-oligo(dG)12-18 and poly(rCm)-oligo (dG)12-18. The control of the polymerase purification by chromatography was performed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis of the pooled active enzymatic fractions.
Bernard
D
D
Laboratoire d'Oncologie Moléculaire, Centre Jean Perrin, Clermont-Ferrand, France.
Moyret
C
C
Maurizis
J C
JC
D001943:Breast Neoplasms / Q000737:chemistry* / Q000473:pathology; D002460:Cell Line; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D004259:DNA-Directed DNA Polymerase / Q000032:analysis / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D011072:Poly U; D012685:Sepharose
D011072:Poly U; D012685:Sepharose; D004259:DNA-Directed DNA Polymerase