Identification of antigens for use in immunodiagnosis of Taenia saginata cysticercosis.
Fifteen metacestode antigens from Taenia saginata were defined by Laurell crossed immunoelectrophoresis and investigated for their potential use in immunodiagnosis of bovine cysticercosis. Several antigens cross reacted with those of some common cattle parasites. Three of the antigens, designated as numbers 4, 8 and 11, were selected on the basis of their restricted cross reactions and were isolated by affinity chromatography. These antigens showed high sensitivity and specificity values in the enzyme-linked immunosorbent assay (ELISA) for serodiagnosis of bovine cysticercosis.
Kamanga-Sollo
E I
EI
Department of Public Health, Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Nairobi, Kenya.
Lindqvist
K J
KJ
Musoke
A J
AJ
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis* / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D003429:Cross Reactions / Q000276:immunology; D003551:Cysticercosis / Q000175:diagnosis / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D007123:Immunoelectrophoresis, Two-Dimensional / Q000379:methods / Q000662:veterinary; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D013621:Taenia / Q000276:immunology*
D000947:Antigens, Helminth