[The experimental mouse model in building an in vitro fertilization center].
Experimental studies on animal models are necessary for the maintenance of practice and experience on IVF and ET, before starting an IVF program. Only so the handling skill of gametes zygote and human embryo culture can be improved. We have started for this reason with IVF on mouse oocytes of CB6F1 Han hybrid strains and used modified Ham's F-10 with 0.75 BSA as culture medium. Until December 1988, 3652 oocytes were collected by the superovulation of 178 oocyte donor mice. The fertilization rate was 67.94%. After the transfer of 62 hybrid embryos obtained from 4 other oocyte donor mice to 4 Balb/c recipient females, 1 recipient mouse delivered 10 living hybrid mice.
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Klinik für Gynäkologie und Geburtshilfe, Ege-Universität Izmir/Türkei.
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Capanoglu
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D000818:Animals; D046508:Culture Techniques / Q000379:methods; D004624:Embryo Transfer / Q000379:methods*; D005314:Embryonic and Fetal Development / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D051379:Mice; D008815:Mice, Inbred Strains; D009865:Oocytes / Q000502:physiology
Das Versuchstiermodell der Maus beim Aufbau eines IVF-Zentrums.