title: Quantitative microscopy of mouse colon 26 cells growing in different metastatic sites.
abstract: Quantitative microdensitometry and computerised interactive image analysis were used to compare the expression of endogenous lectins by cells of mouse colon 26 carcinomas, growing either as primary tumours or metastases, in five different anatomic sites (caecum, liver, lung, spleen, s.c.). Endogenous lectins were visualised in tissue sections using the ABC peroxidase technique with a panel of 17 biotinylated neoglycoproteins representing a variety of carbohydrates found in glycoproteins, glycolipids and proteoglycans. Clear-cut site-associated differences in endogenous lectin expression were detected in cancer cells growing in all five sites. The patterns of these changes were complex and shifts in expression of different lectins were independently variable in both direction and amount. In addition to site-associated variations, differences in lectin expression were also detected in the liver and lungs, between cells in spontaneous metastases and cells in colonies generated by direct injection of cancer cells into the bloodstream. The results demonstrate quantitative, as distinct from qualitative, differences developing in cancer cell populations after delivery of cells to different target organs. The differences between liver and lung metastases are in accord with analogous site-associated differences in metastatic patterns produced by colon carcinoma cells in mice and in humans.
authors.affiliation: Department of Cell Biology and Morphological Sciences, University of the Basque Country, Leioa, Viscaya, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Vidal-Vanaclocha
authors.forename: D
authors.initials: D
authors.lastname: Glaves
authors.forename: E
authors.initials: E
authors.lastname: Barbera-Guillem
authors.forename: L
authors.initials: L
authors.lastname: Weiss
mesh_terms: D000818:Animals; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000737:chemistry* / Q000473:pathology; D005260:Female; D006023:Glycoproteins; D037102:Lectins / Q000032:analysis; D008113:Liver Neoplasms / Q000737:chemistry* / Q000473:pathology / Q000556:secondary; D008175:Lung Neoplasms / Q000737:chemistry* / Q000473:pathology / Q000556:secondary; D051379:Mice; D008807:Mice, Inbred BALB C; D009362:Neoplasm Metastasis; D009605:Nitrosomethylurethane; D014407:Tumor Cells, Cultured
chemical_list: D006023:Glycoproteins; D037102:Lectins; D009605:Nitrosomethylurethane