Selective absorption of anti-DNA antibodies and their idiotype-positive cells in vitro using an anti-idiotypic antibody-affinity column: possible application to plasma exchange.
We examined the possibility of using affinity columns coupled with anti-idiotype (Id) antibodies to selectively remove nephritogenic anti-DNA antibodies in order to determine their possible application to therapeutic plasmapheresis. Monoclonal anti-Id antibodies termed D1E2 or 1F5 were directed to idotypes of human anti-single-stranded and anti-double-stranded DNA antibodies. The mixture of D1E2- and 1F5-coupled Sepharose absorbed 26 to 92% of human anti-DNA antibodies in sera. The affinity columns were also effective in removing anti-DNA idiotype-positive cells from the blood samples of patients, especially those with active lupus nephritis. Thus, an anti-idiotypic antibody-coupled affinity column could, in theory, serve as a tool for selective plasma exchange in the therapy of autoimmune disease.
Harata
N
N
Second Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Sasaki
T
T
Shibata
S
S
Kameoka
J
J
Hirabayashi
Y
Y
Osaki
H
H
Tamate
E
E
Yoshinaga
K
K
D000327:Adsorption; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000974:Antibodies, Antinuclear / Q000097:blood*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000915:Antibody Affinity; D002846:Chromatography, Affinity; D005069:Evaluation Studies as Topic; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000032:analysis; D007163:Immunosorbent Techniques; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000276:immunology; D010951:Plasma Exchange / Q000379:methods*
D000888:Antibodies, Anti-Idiotypic; D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes