Cloning and nucleotide sequence of three genes coding for small, acid-soluble proteins of Clostridium perfringens spores.
Three genes coding for alpha/beta-type small, acid-soluble spore proteins (SASP) have been cloned from Clostridium perfringens and their nucleotide sequence determined. The proteins coded for contain 15 and 21 residues identical to those in Bacillus and C. bifermentans alpha/beta-type SASP, respectively, as well as many homologous residues. However, the C. perfringens SASP exhibit significant sequence divergence from Bacillus and C. bifermentans SASP. These genes may be useful in taxonomic studies of Clostridium species.
Cabrera-Martinez
R M
RM
Department of Biochemistry, University of Connecticut Health Center, Farmington 06032.
Setlow
P
P
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003016:Clostridium perfringens / Q000235:genetics* / Q000502:physiology; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D012808:Sigma Factor; D013171:Spores, Bacterial / Q000502:physiology; D014157:Transcription Factors
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012808:Sigma Factor; D014157:Transcription Factors; C092468:spoIIR protein, Bacillus subtilis; C030069:spore-specific proteins, Bacillus