title: Effects of changes in cell volume on the rates of glutamine and alanine release from rat skeletal muscle in vitro.
abstract: The effect of changes in cell volume on the rates of release of glutamine and alanine from muscle and on the concentrations of these amino acids in muscle were investigated by using an isolated preparation of rat skeletal muscle incubated in the presence of hypo- and hyper-osmotic media. Changes in cell volume were associated with changes in the rates of release of glutamine and alanine from muscle: incubation in hypo-osmotic medium decreased the rates of release of glutamine and alanine, and incubation in hyperosmotic medium increased these rates. These changes were rapidly reversed by a change in osmoticity of the medium. Despite marked changes in cell volume, the concentrations of these amino acids in muscle were maintained. It is suggested that cell volume may play a role in the regulation of amino acid metabolism in skeletal muscle.
authors.affiliation: Department of Biochemistry, University of Oxford, U.K.
authors.forename: M
authors.initials: M
authors.lastname: Parry-Billings
authors.forename: S J
authors.initials: SJ
authors.lastname: Bevan
authors.forename: E
authors.initials: E
authors.lastname: Opara
authors.forename: E A
authors.initials: EA
authors.lastname: Newsholme
mesh_terms: D000409:Alanine / Q000378:metabolism*; D000818:Animals; D005973:Glutamine / Q000378:metabolism*; D006982:Hypertonic Solutions; D007038:Hypotonic Solutions; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D009132:Muscles / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012462:Saline Solution, Hypertonic; D013395:Sucrose / Q000494:pharmacology
chemical_list: D006982:Hypertonic Solutions; D007038:Hypotonic Solutions; D012462:Saline Solution, Hypertonic; D005973:Glutamine; D013395:Sucrose; D000409:Alanine