title: Simplification of procedures used to test urine samples for Chlamydia trachomatis.
abstract: Two methods of simplifying the procedure for examining urine samples for Chlamydia trachomatis were investigated. When 73 urine samples from 56 men with acute non-gonococcal urethritis were examined by direct immunofluorescence (MicroTrak), centrifuging 1 ml volumes of urine at 13,000 rpm for five minutes was at least as efficient for detecting C trachomatis as centrifuging larger volumes at 3000 rpm for 30 minutes. Furthermore, examination of urine produced during a visit to a sexually transmitted disease clinic was at least as efficient as examination of early morning urine for detecting C trachomatis by MicroTrak, or by an enzyme immunoassay (IDEIA). Both modifications have practical advantages and should encourage the use of urine samples for diagnosing chlamydial infections in men.
authors.affiliation: Division of Sexually Transmitted Diseases, Clinical Research Centre, Harrow, Middlesex.
authors.forename: B J
authors.initials: BJ
authors.lastname: Thomas
authors.forename: C
authors.initials: C
authors.lastname: Gilchrist
authors.forename: P E
authors.initials: PE
authors.lastname: Hay
authors.forename: D
authors.initials: D
authors.lastname: Taylor-Robinson
mesh_terms: D001431:Bacteriological Techniques; D002690:Chlamydia Infections / Q000175:diagnosis / Q000652:urine*; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D013997:Time Factors; D014526:Urethritis / Q000382:microbiology / Q000652:urine*; D014556:Urine / Q000382:microbiology