[Bronchoalveolar lavage in patients with systemic scleroderma and systemic lupus erythematosus: characterization of cell activity by cytofluorometry, chemiluminescence and differential cell count].
The purpose of the study was to determine the role of lymphocyte subsets (Ly) and reactive oxygen metabolites RSM, concerning the activity of BAL cells, in the pathogenesis of lung involvement in 12 patients with systemic sclerosis (SS) and 4 with systemic lupus erythematodes (SLE) in comparison with 10 control subjects. The cellular activity was measured by means of cytofluorometry (CFM) and chemiluminescence (CL). In SS/SLEY CD3+, CD4+, CD4/CD8-ratio, CD25 + T-Ly and luminol-dependent CL are increased (p less than 0.05). Correlations exist between CD3+, CD4+, CD8+ and CD25 + T-Ly and both luminol-dependent CL and neutrophils (p less than 0.01). The results suggest, that increased secretion of RSM by BAL-cells may be caused by local release of lymphokines by these activated T-Ly. Therefore CFM and CL seem to be useful in addition to BAL cell differentiation in characterizing the BAL cell activity in the diagnostic of lung involvement in SS and SLE.
Witt
C
C
Klinik für Innere Medizin, Theodor Brugsch, Humboldt-Universität zu Berlin.
Neuhaus
K
K
Winsel
K
K
Brenke
A
A
Hiepe
F
F
Volk
H D
HD
D000328:Adult; D001379:Azathioprine / Q000008:administration & dosage; D016175:B-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007108:Immune Tolerance / Q000187:drug effects / Q000276:immunology; D007958:Leukocyte Count / Q000187:drug effects; D008163:Luminescent Measurements; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis / Q000188:drug therapy / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology; D011239:Prednisolone / Q000008:administration & dosage; D011658:Pulmonary Fibrosis / Q000175:diagnosis / Q000276:immunology*; D012595:Scleroderma, Systemic / Q000175:diagnosis / Q000188:drug therapy / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology*
D011239:Prednisolone; D001379:Azathioprine
Bronchoalveoläre Lavage (BAL) bei Patienten mit systemischer Sklerodermie und systemischem Lupus erythematodes: Charakterisierung der Zellaktivität durch Zytofluorometrie, Chemilumineszenz und Differentialzellbild.