Vitamin K-dependent carboxylase: utilization of decarboxylated bone Gla protein and matrix Gla protein as substrates.
The ability of des-gamma-carboxy bone Gla protein (dBGP) and des-gamma-carboxy matrix Gla protein (dMGP) to act as substrates for the rat liver vitamin K-dependent carboxylase has been investigated. An amino-terminal 'propeptide' is present on the intracellular form of BGP and is thought to interact with a recognition site on the enzyme. dBGP, lacking this extension, is a poor, high apparent Km, carboxylase substrate, but is a much better substrate when free propeptide is added. MGP lacks an amino-terminal propeptide, but contains a a homologous region in the mature protein. dMGP is an excellent substrate for the carboxylase with a low apparent Km and its carboxylation is inhibited by free propeptide.
Engelke
J A
JA
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706.
Hale
J E
JE
Suttie
J W
JW
Price
P A
PA
D000595:Amino Acid Sequence; D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D019735:Carbon-Carbon Ligases; D003619:Dansyl Compounds / Q000378:metabolism; D003653:Decarboxylation; D016326:Extracellular Matrix Proteins; D066298:In Vitro Techniques; D008025:Ligases / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D015675:Osteocalcin / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism; D051381:Rats; D013379:Substrate Specificity; D000097568:Matrix Gla Protein
D002135:Calcium-Binding Proteins; D019735:Carbon-Carbon Ligases; D003619:Dansyl Compounds; D016326:Extracellular Matrix Proteins; D008025:Ligases; D015675:Osteocalcin; D011498:Protein Precursors; D000097568:Matrix Gla Protein; C001026:5-dimethylaminonaphthalene-1-sulfonamide; C020660:glutamyl carboxylase