Comparison of reaction centers from Rhodobacter sphaeroides and Rhodopseudomonas viridis: overall architecture and protein-pigment interactions.
Photosynthetic reaction centers (RCs) from the photosynthetic bacteria Rhodobacter sphaeroides and Rhodopseudomonas viridis are protein complexes closely related in both structure and function. The structure of the Rps. viridis RC was used to determine the structure of the RC from Rb. sphaeroides. Small but meaningful differences between the positions of the helices and the cofactors in the two complexes were identified. The distances between helices AL and AM, between BL and BM, and between bacteriopheophytins BPL and BPM are significantly shorter in Rps. viridis than they are in Rb. sphaeroides RCs. There are a number of differences in the amino acid residues that surround the cofactors; some of these residues form hydrogen bonds with the cofactors. Differences in chemical properties and location of these residues account in some manner for the different spectral properties of the two RCs. In several instances, the hydrogen bonds, as well as the apparent distances between the histidine ligands and the Mg atoms of the bacteriochlorophylls, were found to significantly differ from the Rb. sphaeroides RC structure previously described by Yeates et al. [(1988) Proc. Natl. Acad. Sci. U.S.A. 85, 7993-7997] and Allen et al. [(1988) Proc. Natl. Acad. Sci. U.S.A. 85, 8487-8491].
el-Kabbani
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Biological and Medical Research Division, Argonne National Laboratory, Illinois 60439.
Chang
C H
CH
Tiede
D
D
Norris
J
J
Schiffer
M
M
D000596:Amino Acids / Q000737:chemistry; D001429:Bacteriochlorophylls / Q000737:chemistry*; D001665:Binding Sites; D006860:Hydrogen Bonding; D007501:Iron / Q000378:metabolism; D045342:Light-Harvesting Protein Complexes; D010674:Pheophytins / Q000737:chemistry*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000378:metabolism; D011809:Quinones / Q000378:metabolism; D012242:Rhodobacter sphaeroides / Q000032:analysis*; D012241:Rhodopseudomonas / Q000032:analysis*; D013237:Stereoisomerism; D014961:X-Ray Diffraction
D000596:Amino Acids; D001429:Bacteriochlorophylls; D045342:Light-Harvesting Protein Complexes; D010674:Pheophytins; D045322:Photosynthetic Reaction Center Complex Proteins; D011809:Quinones; C006678:bacteriopheophytin; D007501:Iron