Biochemical and genetic characterization of esterase-27 (ES-27), the major plasma cholinesterase of the house mouse (Mus musculus).
Esterase-27A (ES-27A) was characterized in strain A/WySnA by a cascade of seven bands seen after disc electrophoresis of serum and subsequent staining for esterase. ES-27A catalyses the hydrolysis of thiocholine butyrate and is strongly inhibited by 100 microM tetraisopropyl pyrophosphamide (isoOMPA). Hence, the enzyme was concluded to be a cholinesterase EC 3.1.1.8. A heat-labile form termed ES-27B was represented by strain AKR/Han. From a three-point cross (AKR/Han, A/Wy) and a five-point cross (AKR/Han, SEG/1), the gene order on chromosome 3 was concluded to be centromere-Car-2-Es-26-Es-27-Amy-1-Adh-1.
von Deimling
O H
OH
Abteilung für Chemische Pathologie, Universität Freiburg, Bundesrepublik Deutschland.
Gaa
A
A
D000818:Animals; D002802:Cholinesterases / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D005260:Female; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains
D002802:Cholinesterases