Glycero-3-phosphate is a precursor of glucose but not of glycero-3-phosphocholine in rat liver.
The previous claims by others of a novel choline transfer reaction involving CDP-choline and leading to the conversion of [U-14C]glycero-3-phosphate to glycero-3-phosphocholine in liver sub-cellular fractions could not be confirmed. Apparent labelling of glycero-3-phosphocholine, isolated by paper chromatography, was, in fact, explained by contamination by other radioactive metabolites, especially D-glucose. A synergistic effect on gluconeogenesis from [U-14C]glycero-3-phosphate was obtained by incubating well-washed mitochondria with washed microsomes.
Thompson
W
W
Department of Biochemistry, University of Toronto, Canada.
Belina
H
H
D000818:Animals; D002854:Chromatography, Paper; D005947:Glucose / Q000096:biosynthesis*; D005994:Glycerophosphates / Q000378:metabolism*; D005997:Glycerylphosphorylcholine / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000378:metabolism
D005994:Glycerophosphates; D005997:Glycerylphosphorylcholine; C029620:alpha-glycerophosphoric acid; D005947:Glucose