Cell cycle and electron microscopic evaluation of the adenocarcinoma antigen recognised by the monoclonal antibody 44-3A6.
Adenocarcinomas represent one group of tumours which are seen in the broad spectrum of pulmonary neoplasms. We have produced a monoclonal antibody (44-3A6) which reacts with a 40 kD protein expressed by human primary pulmonary adenocarcinomas and cell lines. Using FACS analysis, this antigen does not appear to be cell cycle specific, and is exposed to the external cell surface. This subcellular localisation has been confirmed using a whole cell, pre-embedding, EM-immunogold labelling technique.
Radosevich
J A
JA
Northwestern University/Veterans Administration, Department of Medicine, Chicago, Illinois 60611.
Siddiqui
F S
FS
Rosen
S T
ST
Kabat
W J
WJ
D000230:Adenocarcinoma / Q000276:immunology*; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000096:biosynthesis*; D000954:Antigens, Surface / Q000096:biosynthesis; D002453:Cell Cycle / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000276:immunology*; D008854:Microscopy, Electron; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface