Okadaic acid blocks membrane fusion of chick embryonic myoblasts in culture.
Okadaic acid was found to block membrane fusion of chick embryonic myoblasts in culture. It also induced morphological change of the cells from bipolar to spherical shape. These effects were dose-dependent, and could be reversed upon removal of the drug from the culture medium. It showed, however, no effect on the induction of muscle specific proteins including tropomyosin and creatine kinase. When okadaic acid was treated to the cell lysates, the phosphorylation state of many proteins significantly increased. These results suggest that the inhibition of myoblast fusion by okadaic acid may be mediated by the increase in the phosphorylation of certain, unknown protein(s) that regulate the fusion process.
Kim
H S
HS
Department of Molecular Biology, College of Natural Sciences, Seoul National University, Korea.
Chung
C H
CH
Kang
M S
MS
Ha
D B
DB
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D003402:Creatine Kinase / Q000096:biosynthesis / Q000302:isolation & purification; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D004988:Ethers, Cyclic / Q000494:pharmacology*; D008561:Membrane Fusion / Q000187:drug effects*; D008970:Molecular Weight; D009132:Muscles / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D019319:Okadaic Acid; D010750:Phosphoproteins / Q000302:isolation & purification; D010766:Phosphorylation
D004988:Ethers, Cyclic; D010750:Phosphoproteins; D019319:Okadaic Acid; D000255:Adenosine Triphosphate; D003402:Creatine Kinase