title: Cytotoxicity of trichothecene mycotoxins isolated from Fusarium sporotrichioides (MC-72083) and Fusarium sambucinum in baby hamster kidney (BHK-21) cells.
abstract: Twenty-six trichothecene mycotoxins produced by Fusarium sporotrichioides (MC-72083) and Fusarium sambucinum were screened for relative cytotoxicity in cultured baby hamster kidney (BHK-21) cells. The relative cytotoxicity was measured as LC100. The most cytotoxic trichothecenes were T-2 toxin (5 ng/ml) and the recently isolated 4-propanoyl HT-2 (5 ng/ml) and 3'-hydroxy T-2 toxin (5 ng/ml). T-2 tetraol (1 x 10(4) ng/ml), 8-beta-hydroxytrichothecene (1 x 10(4) ng/ml), sporotrichiol (2 x 10(4) ng/ml), 8-oxodiacetoxyscirpenol (6 x 10(4) ng/ml) and 8-acetyl T-2 tetraol (1 x 10(5) ng/ml) were the least toxic of the regular trichothecenes. None of the modified trichothecenes or the apotrichothecene were very cytotoxic: 8-beta-hydroxysambucoin (2 x 10(3) ng/ml), FS-1 (5 x 10(3) ng/ml), 8-alpha-hydroxysambucoin (8 x 10(4) ng/ml) and trichotriol (1 x 10(5) ng/ml). The modified trichothecenes, FS-2 and FS-3, were not toxic even at 1 x 10(5) ng/ml. The baby hamster kidney cell bioassay proved to be a very sensitive and reproducible means of screening new trichothecene mycotoxins for relative cytotoxicity.
authors.affiliation: Ethicon Research Foundation, Ethicon, Inc., Somerville, NJ 08876-0151.
authors.forename: L H
authors.initials: LH
authors.lastname: Senter
authors.forename: D R
authors.initials: DR
authors.lastname: Sanson
authors.forename: D G
authors.initials: DG
authors.lastname: Corley
authors.forename: M S
authors.initials: MS
authors.lastname: Tempesta
authors.forename: A A
authors.initials: AA
authors.lastname: Rottinghaus
authors.forename: G E
authors.initials: GE
authors.lastname: Rottinghaus
mesh_terms: D000818:Animals; D001681:Biological Assay; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005670:Fusarium; D015394:Molecular Structure; D015203:Reproducibility of Results; D014255:Trichothecenes / Q000737:chemistry / Q000633:toxicity*
chemical_list: D014255:Trichothecenes