rig encodes ribosomal protein S15. The primary structure of mammalian ribosomal protein S15.
rig, a gene originally isolated from a rat insulinoma cDNA library, codes for a basic 145 amino acid protein [( 1986) Diabetes 35, 1178-1180]. Here we show that the immunoreactivity to a monoclonal antibody against the deduced rig protein and the translation product of rig mRNA comigrated with ribosomal protein S15. The amino acid sequence of ribosomal protein S15 purified from rat liver coincided with that deduced from the nucleotide sequence of rig mRNA, but there were indications that the initiator methionine was removed and the succeeding alanyl residue was monoacetylated. From these results, we conclude that the product of rig is ribosomal protein S15.
Kitagawa
M
M
Department of Biochemistry, Tohoku University School of Medicine, Miyagi, Japan.
Takasawa
S
S
Kikuchi
N
N
Itoh
T
T
Teraoka
H
H
Yamamoto
H
H
Okamoto
H
H
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005796:Genes; D015151:Immunoblotting; D008099:Liver / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D012269:Ribosomal Proteins / Q000032:analysis / Q000235:genetics*; D012270:Ribosomes / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D000911:Antibodies, Monoclonal; D012333:RNA, Messenger; D012269:Ribosomal Proteins; C036004:ribosomal protein S15; D004247:DNA