Characterization of an Onchocerca volvulus cDNA clone encoding a genus specific antigen present in infective larvae and adult worms.
The isolation and characterization of a recombinant cDNA clone (OV7) expressing an antigen present in Onchocerca volvulus infective larvae and adult stages is described. Using chimpanzee antiserum generated against irradiated infective larvae, we isolated a cDNA clone from a lambda gt11 cDNA expression library derived from adult O. volvulus mRNA. The open reading frame encodes 131 amino acids corresponding to a 15.2-kDa protein. Affinity purified antibodies which bound specifically to OV7 fusion polypeptide recognized a single antigen with an apparent molecular weight of 17,000 in extracts of L3, L4 and adult worms. Immunoelectron microscopy established that the antigen encoded by this clone is present in the hypodermis and the basal layer of the cuticle of L3 and female adult worm, and in the egg shell around developing microfilariae. Since the OV7 fusion polypeptide is onchocerca-specific and is recognized specifically by sera from onchocerciasis patients, and sera from non-patent but infected chimpanzees, and not by sera from patients with other filarial parasites, it may have potential as an antigenic component in a test for detection of non-patent and patent infections of O. volvulus. The OV7 amino acid sequence contains residues that have a probable homology with the cysteine proteinase inhibitor superfamily.
Lustigman
S
S
Lindsley F. Kimball Research Institute, New York Blood Center, New York 10021.
Brotman
B
B
Huima
T
T
Prince
A M
AM
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000947:Antigens, Helminth / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D015891:Cystatins / Q000235:genetics; D004247:DNA / Q000032:analysis*; D005260:Female; D006801:Humans; D007814:Larva; D008297:Male; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009854:Onchocerca / Q000235:genetics* / Q000276:immunology / Q000648:ultrastructure; D009855:Onchocerciasis / Q000175:diagnosis; D016366:Open Reading Frames; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D000906:Antibodies; D000947:Antigens, Helminth; D015891:Cystatins; D011994:Recombinant Proteins; D004247:DNA