Replication of TMV-L and Lta1 RNAs and their recombinants in TMV-resistant Tm-1 tomato protoplasts.
Tm-1 is a gene that provides resistance to tomato plants against tobacco mosaic virus (TMV) infection. In tomato cells carrying the Tm-1 gene, multiplication of TMV is inhibited. From previous analysis of resistance-breaking mutants, the involvement of the 130- and 180-kDa proteins, putative viral replicases, in the resistance conferred by the Tm-1 gene was suggested. When wild-type TMV RNA was co-inoculated with a resistance-breaking mutant RNA, replication of the wild-type TMV genomic RNA could not be rescued by the 130- and 180-kDa proteins of a resistance-breaking strain, Lta1. To investigate how the putative resistance factor interacts with the 130- and 180-kDa proteins, we expressed the wild-type TMV protein sequence that is associated with the resistance-breaking phenomenon as part of a recombinant virus derived from Lta1 in Tm-1/Tm-1 protoplasts. No specific degradation of wild-type TMV protein sequences was observed, suggesting that the mechanism of the resistance does not involve the instability of a viral protein.
Yamafuji
R
R
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
Watanabe
Y
Y
Meshi
T
T
Okada
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D036022:Capsid Proteins; D017343:Genes, Plant; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010935:Plant Diseases; D011523:Protoplasts / Q000382:microbiology*; D012367:RNA, Viral / Q000096:biosynthesis*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D011995:Recombination, Genetic; D014027:Tobacco Mosaic Virus / Q000235:genetics*; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics*; D014779:Virus Replication / Q000235:genetics*
D036022:Capsid Proteins; C009496:H protein, Tobacco mosaic virus; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins