Use of the deoxyuridine suppression test to evaluate localized folate deficiency in rat colonic epithelium.
In this study the deoxyuridine suppression test (dUST) was performed on isolated rat colonocytes to establish its value as an indicator of folate status in the colonic epithelium. [3H]thymidine incorporation into DNA was suppressed greater than 90% by deoxyuridine (dU) concentrations greater than 2.5 mumol/L. Preincubation of cells with 5-fluorouracil (1-100 mumol/L) but not methotrexate (10-100 mumol/L) resulted in a significant decrease in the degree of suppression. The dUST performed on colonocytes from folate-deficient animals displayed less suppression than on colonocytes from folate-replete animals (P less than 0.05). The abnormal degree of suppression was corrected by adding 100 mumol folinic acid/L. There was a negative correlation between the degree of suppression and the folate concentration of the colonic epithelium (P less than 0.001). These data indicate that the dUST is useful for detecting folate deficiency in the colonic epithelium and may therefore be valuable in assessing a deficiency state localized to that epithelium.
Cravo
M L
ML
Vitamin Bioavailability Laboratory, US Department of Agriculture Human Nutrition Research Center on Aging, Tufts University, Boston.
Mason
J B
JB
Selhub
J
J
Rosenberg
I H
IH
D000818:Animals; D002478:Cells, Cultured; D003106:Colon / Q000737:chemistry* / Q000166:cytology; D004247:DNA / Q000378:metabolism; D003857:Deoxyuridine; D004848:Epithelium / Q000737:chemistry; D005472:Fluorouracil / Q000378:metabolism; D005492:Folic Acid / Q000032:analysis*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013936:Thymidine / Q000378:metabolism
D004247:DNA; D005492:Folic Acid; D005472:Fluorouracil; D013936:Thymidine; D003857:Deoxyuridine