Ether anesthesia differentially affects the content of prepro-corticotropin-releasing hormone, prepro-neurotensin/neuromedin N and prepro-enkephalin mRNAs in the hypothalamic paraventricular nucleus of the rat.
The content of the mRNAs that code for prepro-corticotropin releasing hormone (ppCRH), prepro-neurotensin/neuromedin N (ppNT/NMN) and prepro-enkephalin (ppENK) was measured using in situ hybridization histochemistry in the various subdivisions of the hypothalamic paraventricular nucleus (PVH) 4 h after a bout of ether anesthesia. This treatment increased the content of ppENK mRNA only in a selected region of the dorsal medial parvicellular PVH (PVHmpd). PpNT/NMN mRNA was undetectable in the PVH of control animals, but hybridization was observed in the PVHmpd of anesthetized animals. The content of ppCRH mRNA was unchanged 4 h after ether aesthesia.
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Neural Systems Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037.
D000758:Anesthesia; D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000235:genetics* / Q000378:metabolism; D004745:Enkephalins / Q000235:genetics* / Q000378:metabolism; D004987:Ethers / Q000494:pharmacology*; D008297:Male; D009496:Neurotensin / Q000235:genetics* / Q000378:metabolism; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism*; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats
D004745:Enkephalins; D004987:Ethers; D011498:Protein Precursors; D012333:RNA, Messenger; C052457:neuromedin N precursor; C041645:pro-corticotropin releasing hormone; D009496:Neurotensin; D003346:Corticotropin-Releasing Hormone; C033660:preproenkephalin