title: Antioxidant properties of the alkaloid boldine in systems undergoing lipid peroxidation and enzyme inactivation.
abstract: Boldine, in low micromolar concentrations, was able to prevent brain homogenate autooxidation, the 2,2'-azobis(2-amidinopropane)(AAP)-induced lipid peroxidation of red cell plasma membranes, and the AAP-induced inactivation of lysozyme. These results are indicative of a high reactivity of boldine towards free radicals. The analysis of the boldine effect as a function of incubation times suggests that a metabolite resulting from the interaction of boldine with free radicals also exhibits antioxidant activity, being more efficient than boldine in brain homogenate auto-oxidation and less efficient in lysozyme protection experiments. This behavior may be accounted for in terms of the relative location of the scavengers needed to afford maximal protection.
authors.affiliation: Facultad de Química, Pontificia Universidad Católica, Santiago, Chile.
authors.forename: H
authors.initials: H
authors.lastname: Speisky
authors.forename: B K
authors.initials: BK
authors.lastname: Cassels
authors.forename: E A
authors.initials: EA
authors.lastname: Lissi
authors.forename: L A
authors.initials: LA
authors.lastname: Videla
mesh_terms: D000578:Amidines / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000737:chemistry*; D001060:Aporphines / Q000737:chemistry* / Q000494:pharmacology; D001923:Brain Chemistry; D004305:Dose-Response Relationship, Drug; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D015227:Lipid Peroxidation; D008163:Luminescent Measurements; D008297:Male; D009113:Muramidase / Q000037:antagonists & inhibitors; D010101:Oxygen Consumption; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000578:Amidines; D000975:Antioxidants; D001060:Aporphines; C046728:2,2'-azobis(2-amidinopropane); C011686:boldine; D009113:Muramidase