The perinatal development of glucose-6-phosphatase activity distribution pattern in rat liver. A microdensitometrical study.
The ontogenetic development of the intralobular distribution pattern of glucose-6-phosphatase activity in the rat liver is described in terms of histochemical changes determined with microdensitometry. A newly developed cerium-lead technique was employed and compared with the common lead technique optimized by Teutsch (1978a). The cerium technique has advantages, meets the prerequisites for quantitative determinations and yields results comparable to biochemically obtained data from microdissected tissue. The first signs of a heterogeneous distribution pattern of glucose-6-phosphatase activity are observed on the 3rd d after birth, and differences between periportal and centrolobular areas are largest around 10th and 15th d. At 30th d after birth, the adult pattern is complete with a centrolobular glucose-6-phosphatase activity of 67% of the periportal value.
Klinger
M H
MH
Department of Histochemistry, Friedrich Schiller University Jena, FRG.
Halbhuber
K J
KJ
D000375:Aging; D000818:Animals; D002563:Cerium; D005260:Female; D005865:Gestational Age; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006651:Histocytochemistry; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000196:embryology / Q000201:enzymology / Q000254:growth & development*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D002563:Cerium; D005954:Glucosephosphate Dehydrogenase