Characterization of the Opisthorchis viverrini genome.
The methylations of trematode genomic DNA were analyzed using restriction enzymes and Southern blot hybridization. Restriction enzymes MspI, HpaII, HhaI were used to probe CpG methylation while MboI, Sau3A, DpnI were used for A methylation. The results revealed that Opisthorchis viverrini, Fasciola gigantica and Gigantocotyle siamensis had neither CpG nor A methylations. The presence of highly repeated DNA elements was also demonstrated in O. viverrini genomic DNA.
Sermswan
R
R
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Mongkolsuk
S
S
Sirisinha
S
S
D000818:Animals; D015139:Blotting, Southern; D004247:DNA / Q000032:analysis / Q000378:metabolism*; D015342:DNA Probes; D008745:Methylation; D009891:Opisthorchis / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D015342:DNA Probes; D004247:DNA