title: Overproduction and purification of McrC protein from Escherichia coli K-12.
abstract: The McrC protein, encoded by one of the two genes involved in the McrB restriction system, was produced in Escherichia coli cells by using a T7 expression system. Following sequential DEAE-Sepharose and hydroxylapatite column chromatography, the protein was purified to apparent homogeneity as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The N-terminal amino acid sequence of the purified McrC protein agreed exactly with the one deduced from the DNA sequence by Ross et al. (J. Bacteriol. 171:1974-1981, 1989).
authors.affiliation: Department of Microbiology, Louisiana State University, Baton Rouge 70803.
authors.forename: L
authors.initials: L
authors.lastname: Zheng
authors.forename: H D
authors.initials: HD
authors.lastname: Braymer
mesh_terms: D001345:Autoradiography; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D015257:DNA-Cytosine Methylases / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D016366:Open Reading Frames; D010957:Plasmids; D015183:Restriction Mapping
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C069326:mcrC protein, E coli; C059683:DNA modification methylase McrB; D015257:DNA-Cytosine Methylases; D004262:DNA Restriction Enzymes