Recognition sequence of a new methyl-specific restriction system from Clostridium acetobutylicum strain ABKn8.
A new type II restriction endonuclease, named Cac8I was detected in Clostridium acetobutylicum strain ABKn8. Cac8I cleaved the hexanucleotide sequence [5'-GCN decreases NGC-3'] and generated blunt ends. Up to now no isoschizomer of Cac8I has been described [corrected].
Azeddoug
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Unité des Anaérobies, Institut Pasteur, Paris, France.
Reysset
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D001483:Base Sequence; D003001:Cloning, Molecular; D003013:Clostridium / Q000201:enzymology*; D015254:DNA Modification Methylases / Q000302:isolation & purification / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D010957:Plasmids
D015254:DNA Modification Methylases; C069113:endodeoxyribonuclease CacII; D015252:Deoxyribonucleases, Type II Site-Specific