Improved high-performance liquid chromatographic assay for the determination of ethionamide in serum.
A solid-phase extraction (SPE) method was developed to simplify the preparation of human serum prior to high-performance liquid chromatography of ethionamide (ETA). Octadecyl SPE columns were used. Serum constituents were removed from the column with water, and ETA was eluted with methanol. Samples were evaporated to dryness, reconstituted in mobile phase, and assayed. The method is reproducible, with a recovery of ETA of 64%, comparable to the more tedious liquid-liquid extraction method for ETA.
Peloquin
C A
CA
Department of Medicine, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.
James
G T
GT
McCarthy
E
E
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005000:Ethionamide / Q000097:blood*; D006801:Humans; D000432:Methanol
D005000:Ethionamide; D000432:Methanol