title: Effect of respiratory infection on tissue riboflavin and flavin enzymes in mice.
abstract: The effects of Klebsiella pneumoniae infection on red blood cell and liver riboflavin levels and a few flavin enzymes were examined to verify our earlier hypothesis based on human experience that upper respiratory infections result in mobilization of riboflavin from tissues into blood, resulting in a rise in red blood cell riboflavin and an increased saturation of erythrocyte glutathione reductase (EGR) with its coenzyme flavin adenine dinucleotide (FAD; reduction in EGR activation coefficient, AC, values). Thirty-six-day-old male mice fed a diet marginally sufficient in riboflavin were injected with a single sublethal dose of K. pneumoniae. Batches of control, deficient infected and deficient uninfected animals were killed during the peak period of infection and after recovery. Infection brought about a significant rise in basal EGR activity due to greater saturation with coenzyme FAD and a concomitant reduction in EGR-AC values. On recovery, basal EGR activity decreased, and EGR-AC values increased to levels beyond control values, suggesting riboflavin deficiency. In the liver, acyl-coenzyme A dehydrogenase showed a significant decrease and pyridoxamine-phosphate oxidase activity a significant increase. Both the enzymes normalized after recovery. D-amino acid oxidase activity did not change.
authors.affiliation: National Institute of Nutrition, Indian Council of Medical Research, Hyderabad, India.
authors.forename: P A
authors.initials: PA
authors.lastname: Prasad
authors.forename: A V
authors.initials: AV
authors.lastname: Lakshmi
authors.forename: P
authors.initials: P
authors.lastname: Suresh
authors.forename: M S
authors.initials: MS
authors.lastname: Bamji
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism; D005182:Flavin-Adenine Dinucleotide / Q000097:blood; D005415:Flavins / Q000378:metabolism*; D005980:Glutathione Reductase / Q000097:blood; D007710:Klebsiella Infections / Q000378:metabolism; D007711:Klebsiella pneumoniae; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008297:Male; D051379:Mice; D012141:Respiratory Tract Infections / Q000201:enzymology / Q000378:metabolism* / Q000382:microbiology; D012256:Riboflavin / Q000097:blood / Q000378:metabolism*
chemical_list: D005415:Flavins; D005182:Flavin-Adenine Dinucleotide; D005980:Glutathione Reductase; D012256:Riboflavin