title: A convenient approach to the synthesis of medium size oligodeoxyribonucleotides by improved new phosphite method.
abstract: Improvement of the new phosphite method for the synthesis of oligodeoxyribonucleotides using the deoxyribonucleoside 3'-bis(1,1,1,3,3,3- hexafluoro-2-propyl) phosphite unit has been carried out via the hydrolysis and capping steps, without any side reaction products. The new phosphite unit and capping agent, bis(1,1,1,3,3,3-hexafluoro-2-propyl)-2-propyl phosphite, is readily activated by N-methylimdazole under very mild condition on a solid support. This operation involves a one pot reaction, which is an advantage over both the phosphite and H- phosphonate approaches. The mechanism of internucleotidic bond formation of the new phosphite method is also discussed.
authors.affiliation: Department of Industrial Chemistry, Chiba Institute of Technology, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Hosaka
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Sato
authors.forename: S
authors.initials: S
authors.lastname: Gug-Kim
authors.forename: H
authors.initials: H
authors.lastname: Takaku
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D009682:Magnetic Resonance Spectroscopy; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D009943:Organophosphorus Compounds / Q000737:chemistry*; D017905:Phosphites; D010759:Phosphorus Isotopes; D005466:Fluorocarbons
chemical_list: D009838:Oligodeoxyribonucleotides; D009943:Organophosphorus Compounds; D017905:Phosphites; D010759:Phosphorus Isotopes; C069480:3'-bis(1,1,1,3,3,3-hexafluoro-2-propyl)phosphite; D005466:Fluorocarbons