Improved procedure for bacteriophage typing of Listeria strains and evaluation of new phages.
A modified technique in listerial phage typing, termed reversed phage typing procedure, was developed. Ready-to-use typing plates were prepared by preapplication of phage suspensions on tryptose agar plates. The new procedure offers a number of substantial advantages as compared with the conventional method, being much more reliable, efficient, and convenient to use. More than 1,000 strains of Listeria have thus far been typed with the reversed phage typing procedure, employing an extended set of 21 genus-specific bacteriophages. The overall typability of strains was 89.5%.
Loessner
M J
MJ
Institute of Bacteriology, Technical University of Munich, Freising, Federal Republic of Germany.
D015373:Bacterial Typing Techniques; D001435:Bacteriophages / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D008087:Listeria / Q000145:classification*; D013045:Species Specificity