title: 2,4-Dichlorophenoxyacetic acid causes chromatin and chromosome abnormalities in plant cells and mutation in cultured mammalian cells.
abstract: The cytotoxic and mutagenic effects of the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) on shallot root tip cells and on V79 Chinese hamster fibroblast cells were examined and compared. In shallot root tips 2,4-D caused changes in mitotic activity, as well as changes in chromosome and chromatin structure, and also changes during the cell cycle. 2,4-D also showed mutagenic and cytotoxic effects on V79 cells in culture in concentrations higher than 10 micrograms/ml. The results in both systems (plant and mammalian cells) were in agreement showing mutagenic activity of 2,4-D in the concentration range higher than usually used in establishing plant tissue culture (greater than 5 micrograms/ml).
authors.affiliation: Department of Molecular Biology, Faculty of Science, University of Zagreb, Yugoslavia.
authors.forename: M
authors.initials: M
authors.lastname: Pavlica
authors.forename: D
authors.initials: D
authors.lastname: Papes
authors.forename: B
authors.initials: B
authors.lastname: Nagy
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000633:toxicity*; D000490:Allium; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002843:Chromatin / Q000187:drug effects / Q000648:ultrastructure; D002869:Chromosome Aberrations; D005347:Fibroblasts; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D009152:Mutagenicity Tests; D009154:Mutation; D008941:Spindle Apparatus / Q000187:drug effects; D013866:Thioguanine / Q000494:pharmacology
chemical_list: D002843:Chromatin; D015084:2,4-Dichlorophenoxyacetic Acid; D013866:Thioguanine