Macrophage subsets in the rat gut: an immunohistochemical and enzyme-histochemical study.
Macrophages' subsets including dendritic cells were characterized in situ and in cell suspensions of Peyer's patches, intestinal villi, and mesenteric lymph nodes using monoclonal antibodies, lectins, and enzyme-histochemistry. By combined labelling procedures, it was found that Peyer's patches comprise dendritic cells in addition to 2 types of macrophages; dendritic cells were not observed among the various macrophage-like cells in the lamina propria of the villi. The lectins Dolichos biflorus and Griffonia simplicifolia I agglutinin appeared to be useful for discriminating between several subtypes of macrophages.
Sminia
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Department of Cell Biology, Medical Faculty, Vrije Universiteit, Amsterdam, The Netherlands.
van der Ende
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MB
D000818:Animals; D000911:Antibodies, Monoclonal; D002241:Carbohydrates / Q000032:analysis; D007124:Immunoenzyme Techniques; D007421:Intestine, Small / Q000166:cytology*; D037102:Lectins; D008198:Lymph Nodes / Q000166:cytology; D008264:Macrophages / Q000166:cytology*; D008297:Male; D010581:Peyer's Patches / Q000166:cytology*; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D037102:Lectins