Studies on N-acetylglucosaminidase activity produced by Streptomyces hygroscopicus.
Following growth on chitin-containing media the actinomycete, Streptomyces hygroscopicus produces N-acetylglucosaminidase. When the organism was grown in submerged culture on chitin-containing media, at 30 degrees C, production of the N-acetylglucosaminidase activity has been shown to increase to a maximum at around day 18. Following electrophoretic analysis of culture filtrates and subsequent zymogram staining with fluorescent substrate analogues, at least three general pools of N-acetylglucosaminidase activity were detected. These were named NA1, NA2 and NA3. In addition, a potential chitinase was also identified. The N-acetylglucosaminidases, referred to above as NA1 and NA2, were separated and their enzymatic properties were investigated.
Irhuma
A
A
Department of Microbiology, Moyne Institute, Trinity College, Dublin, Republic of Ireland.
Gallagher
J
J
Hackett
T J
TJ
McHale
A P
AP
D000118:Acetylglucosaminidase / Q000378:metabolism*; D002686:Chitin / Q000378:metabolism; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology* / Q000254:growth & development; D013696:Temperature
D003470:Culture Media; D008670:Metals; D002686:Chitin; D000118:Acetylglucosaminidase