The behavior of human ameloblastoma tissue in collagen matrix in vitro: an immunohistochemical study.
We investigated the three-dimensional outgrowth of human ameloblastoma cells cultured in collagen matrix. Growth patterns of four cases of ameloblastoma resembled each other and was characterized by appeared duct-like structures. Case 1 ameloblastoma was subcultured 3 times. Ameloblastoma tissues and cultured cells were stained with various lectins by avidin-biotin peroxidase complex (ABC) staining methods. Both the tissues and cultured cells had the same receptors to Concanavalin ensiforme (Con A), Ricinus communis Agglutinin (RCA-I) and Triticum vulgaris (WGA) but not to Dolichos biflorus (DBA), Ulex europaeus (UEA-1), Soybean (SBA) and Arachis hypogea (PNA). These findings indicated that the cultured cells examined in this study were ameloblastoma cells in origin.
Yasuda
K
K
First Department of Oral and Maxillofacial Surgery, School of Dentistry, Tokushima University, Japan.
Nakanishi
A
A
Satomura
K
K
Nagayama
M
M
Hayashi
Y
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D000564:Ameloblastoma / Q000276:immunology / Q000473:pathology*; D003094:Collagen; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000276:immunology / Q000473:pathology*; D011975:Receptors, Mitogen; D014407:Tumor Cells, Cultured
D011975:Receptors, Mitogen; D003094:Collagen