title: Cyclosporine determinations in heart and liver transplant recipients: comparison of HPLC and FPIA methods on whole blood and plasma.
abstract: One hundred and eighty five whole blood samples were analysed for cyclosporine levels by fluorescence polarization immunoassay (FPIA) and high performance liquid chromatography (HPLC). 123 came from 4 heart transplant recipients (mean age +/- SD: 47.50 +/- 20.56 years) and 62 from 4 liver transplant recipients (44.50 +/- 16.52 years). FPIA was done on plasma and whole blood in heart transplant recipients, on plasma in the liver recipients. HPLC was always done on whole blood. The results show a good correlation between FPIA on plasma (y) and HPLC (x) in liver recipients (n = 62, r = 0.935, y = 1.23x + 70 ng/ml), slightly worse between FPIA on plasma (y) and HPLC (x) in heart recipients (n = 64, r = 0.610, y = 0.78x + 189 ng/ml) and mediocre for FPIA on whole blood (y) and HPLC (x) in heart recipients (n = 123, r = 0.566, y = 1.35x + 594 ng/ml).
authors.affiliation: Service de Pharmacologie Clinique et de Toxicologie du C.H.U. de Dijon,
authors.forename: C
authors.initials: C
authors.lastname: Corda
authors.forename: A
authors.initials: A
authors.lastname: Escousse
authors.forename: L
authors.initials: L
authors.lastname: Santona
authors.forename: F
authors.initials: F
authors.lastname: Guédon
authors.forename: J
authors.initials: J
authors.lastname: Mounié
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid; D003524:Cyclosporins / Q000097:blood*; D005260:Female; D016231:Fluorescence Polarization Immunoassay; D016027:Heart Transplantation; D006801:Humans; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D011184:Postoperative Period
chemical_list: D003524:Cyclosporins