Successful cryopreservation of human bone marrow does not require a controlled-rate freezer.
We studied CFU-GM recovery from bone marrow samples frozen either in a machine for controlled-rate freezing or in a -70 degrees C freezer. We found no statistically significant difference between the two methods. Cooling-curve investigations also demonstrated that it was possible to obtain satisfactory cooling rates simply by manipulation of the shape and volume of the marrow and its container. Five patients received a total of six successful autografts for which their marrow cells were frozen without controlled-rate freezing. Thus it is not necessary to use a sophisticated machine in order to obtain satisfactory cryopreservation of human marrow stem cells.
Clark
J
J
Department of Haematology, Charing Cross and Westminster Medical School, Charing Cross Hospital, London, UK.
Pati
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McCarthy
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D001853:Bone Marrow / Q000473:pathology; D001854:Bone Marrow Cells; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods*; D005615:Freezing; D006801:Humans; D013997:Time Factors