title: Isolation and characterization of two different molecular forms of basic fibroblast growth factor extracted from human placental tissue.
abstract: Basic fibroblast growth factor (bFGF) was purified to homogeneity from human placental tissue on a semi-large scale. Placental bFGF consists of two proteins of apparent molecular masses 16,000 and 18,000 dalton, as determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis under non-reducing conditions. Microsequence analysis showed that both proteins have the same N-terminal sequence Pro-Ala-Leu-Pro-Glu-Asp-Gly-Gly-Ser-Gly-Ala-Phe..., which is identical with that of (1-146) bFGF extracted from human brain. After reduction by dithiothreitol or mercaptoethanol, placental bFGF appears as a single protein of 16,000 dalton. The reduced protein displays the same ability to stimulate the proliferation of CCL39 fibroblasts as the non-reduced doublet. These data indicate that bFGF extracted from placental tissue consists of two proteins with different apparent molecular masses which do not differ in their N-terminal sequence but in their oxidation state.
authors.affiliation: IMEDEX, Z.I. Les Troques, Chaponost, France.
authors.forename: S
authors.initials: S
authors.lastname: Uhlrich
authors.forename: J
authors.initials: J
authors.lastname: Tiollier
authors.forename: M
authors.initials: M
authors.lastname: Tardy
authors.forename: J L
authors.initials: JL
authors.lastname: Tayot
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D004229:Dithiothreitol / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000032:analysis / Q000302:isolation & purification*; D006801:Humans; D007460:Iodoacetamide / Q000494:pharmacology; D008623:Mercaptoethanol / Q000494:pharmacology; D008969:Molecular Sequence Data; D010920:Placenta / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D016222:Fibroblast Growth Factor 2; D008623:Mercaptoethanol; D004229:Dithiothreitol; D007460:Iodoacetamide