Ultrastructural characterization of human immunodeficiency virus type 1 Gag-containing particles assembled in a recombinant adenovirus vector system.
The human immunodeficiency virus type 1 (HIV-1) Gag protein was expressed in A549 cells infected with recombinant adenovirus types 4 and 7, each carrying the HIV-1 gag and pro genes. The Gag protein was assembled into enveloped virus-like particles that budded from plasma and vacuolar membranes. The particles, isolated by precipitation and isopycnic density centrifugation, contained both processed and unprocessed Gag-associated proteins.
Vernon
S K
SK
Biotechnology and Microbiology Division, Wyeth-Ayerst Research, Philadelphia, Pennsylvania 19101.
Murthy
S
S
Wilhelm
J
J
Chanda
P K
PK
Kalyan
N
N
Lee
S G
SG
Hung
P P
PP
D000260:Adenoviruses, Human / Q000235:genetics*; D002460:Cell Line; D015683:Gene Products, gag / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure*; D005822:Genetic Vectors; D015497:HIV-1 / Q000235:genetics / Q000648:ultrastructure*; D006801:Humans; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D011995:Recombination, Genetic
D015683:Gene Products, gag