Crystallization and preliminary X-ray analysis of peanut agglutinin-N6-benzylaminopurine complex.
Preliminary diffraction data collected on peanut agglutinin (PNA) crystals grown in the presence of N6-benzylaminopurine (BAP) indicate a monoclinic cell (P2) with a = 67.0 A, b = 35.2 A, c = 65.8 A and beta = 68.6 degrees. This is the first example of a legume lectin crystallized with a bound phytohormone. Crystals of PNA grown previously in the presence of lactose had an orthorhombic space group (P2(1)2(1)2) with a = 128.8 A, b = 126.0 A and c = 76.1 A and one tetramer per asymmetric unit. The Vm value for the PNA-BAP crystals is 2.62 A3/Da, assuming one monomer of PNA per asymmetric unit. Thus, while the PNA-lactose complex crystallized as tetramers, the PNA-BAP complex has, at most, dimers in the crystal. These results indicate that BAP, a naturally occurring phytohormone, can modify the quaternary structure of PNA by dissociation and change its carbohydrate valence.
Zaluzec
E J
EJ
Department of Chemistry, Loyola University of Chicago, IL 60626.
Zaluzec
M M
MM
Olsen
K W
KW
Pavkovic
S F
SF
D000225:Adenine / Q000031:analogs & derivatives* / Q000737:chemistry; D010367:Arachis; D001593:Benzyl Compounds; D003460:Crystallization; D007701:Kinetin; D037102:Lectins / Q000737:chemistry* / Q000302:isolation & purification; D008968:Molecular Conformation; D019887:Peanut Agglutinin; D010937:Plant Growth Regulators / Q000737:chemistry; D037121:Plant Lectins; D011487:Protein Conformation; D011687:Purines; D014961:X-Ray Diffraction / Q000379:methods
D001593:Benzyl Compounds; D037102:Lectins; D019887:Peanut Agglutinin; D010937:Plant Growth Regulators; D037121:Plant Lectins; D011687:Purines; C068942:peanut agglutinin-N(6)-benzylaminopurine complex; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin