title: Culture of human outer root sheath cells from plucked hair follicles in serum-free conditions.
abstract: We succeeded in culturing human outer root sheath cells (ORSC) in serum-free conditions without a biological feeder layer. The combination of collagen type IV substrate and modified MCDB 153 medium supplemented with bovine pituitary gland extract (K-GM medium) could support the growth of ORSC. These cells can be serially cultivated for at least 4 passages and stored in liquid nitrogen with good recovery. Thus, a large series of experiments using ORSC may be run simultaneously.
authors.affiliation: Department of Dermatology, School of Medicine, University of Tokushima, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Arase
authors.forename: S
authors.initials: S
authors.lastname: Katoh
authors.forename: Y
authors.initials: Y
authors.lastname: Sadamoto
authors.forename: H
authors.initials: H
authors.lastname: Nakanishi
authors.forename: Y
authors.initials: Y
authors.lastname: Urano
authors.forename: K
authors.initials: K
authors.lastname: Fujie
authors.forename: K
authors.initials: K
authors.lastname: Takeda
mesh_terms: D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen; D003114:Colony-Forming Units Assay; D003470:Culture Media; D006197:Hair / Q000166:cytology*; D006801:Humans
chemical_list: D003470:Culture Media; D003094:Collagen