Fast-twitch and slow-twitch skeletal muscles express the same isoform of calreticulin.
We have characterized calreticulin protein and mRNA in fast-twitch and slow-twitch skeletal muscle. SR vesicles isolated from fast-twitch and slow-twitch skeletal muscles contain calreticulin protein immunoreactive with anti-calreticulin antibody. In addition, the fast-twitch skeletal muscle form of calreticulin is shown to be identical to slow-twitch form of the protein based on the identity of cDNA clones encoding fast-twitch and slow-twitch forms of the protein and hybridization of a fast-twitch or slow-twitch calreticulin cDNA probe to the same mRNA species. Based on these observations we conclude that fast-twitch and slow-twitch skeletal muscles express the same form of calreticulin.
Fliegel
L
L
Department of Biochemistry, University of Alberta, Edmonton, Canada.
Michalak
M
M
D000595:Amino Acid Sequence; D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000302:isolation & purification; D037282:Calreticulin; D003001:Cloning, Molecular; D015342:DNA Probes; D004591:Electrophoresis, Polyacrylamide Gel; D015723:Gene Library; D015151:Immunoblotting; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009132:Muscles / Q000502:physiology*; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D011817:Rabbits; D015183:Restriction Mapping; D012519:Sarcoplasmic Reticulum / Q000502:physiology*; D012689:Sequence Homology, Nucleic Acid
D002135:Calcium-Binding Proteins; D037282:Calreticulin; D015342:DNA Probes; D012333:RNA, Messenger