title: Lipoproteins do not influence cholesterol synthesis in freshly isolated rat hepatocytes, cautionary note.
abstract: 1. Suspensions of freshly isolated rat hepatocytes were used to study the effects of native and derivatized lipoproteins on the rate of cholesterogenesis. 2. Short-term incubation of the hepatocytes with a variety of lipoproteins failed to modify the rate of cholesterol synthesis as determined by the incorporation of tritium from tritiated water into cholesterol after separation from other lipids by thin-layer chromatography. 3. Neither an increase in the cholesterol content of the particles (beta-very-low-density lipoproteins) nor derivatization of the lipoproteins (lactosylated-low-density lipoproteins or high-density lipoproteins associated with a tris-galactoside-terminated cholesterol derivative) nor cholesterol-containing liposomes were effective in this respect. 4. Whether this behaviour represents an artefact of the isolated hepatocyte preparation is unknown yet.
authors.affiliation: Laboratory of Veterinary Biochemistry, University of Utrecht, The Netherlands.
authors.forename: M J
authors.initials: MJ
authors.lastname: Geelen
authors.forename: A C
authors.initials: AC
authors.lastname: Beynen
authors.forename: C H
authors.initials: CH
authors.lastname: Paays
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000096:biosynthesis* / Q000494:pharmacology; D002914:Chylomicrons / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis; D007700:Kinetics; D008074:Lipoproteins / Q000494:pharmacology*; D008079:Lipoproteins, VLDL / Q000494:pharmacology; D008081:Liposomes; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium
chemical_list: D002914:Chylomicrons; D005227:Fatty Acids; D008074:Lipoproteins; D008079:Lipoproteins, VLDL; D008081:Liposomes; D014316:Tritium; D002784:Cholesterol