title: Total degradation of various chlorobiphenyls by cocultures and in vivo constructed hybrid pseudomonads.
abstract: Cocultures consisting of strains converting chlorobiphenyls to the respective benzoates or catechols and of chlorobenzoate degraders were investigated for the mineralization of chlorobiphenyls. Stable mixed cultures were obtained with 4-chlorobiphenyl, while those with 2-chloro- or 3-chlorobiphenyl were found to be unstable and released only low yields of chloride. When both sets of enzyme sequences were combined in one organism, Pseudomonas cepacia strain JH230, by conjugative transfer of genes of the biphenyl degradation sequence, the total degradation of 2-chloro-, 3-chloro-, 4-chloro-, 2,4-dichloro-, and 3,5-dichlorobiphenyl was achieved.
authors.affiliation: Bergische Universität, Gesamthochschule Wuppertal, Chemische Mikrobiologie, F.R.G.
authors.forename: J
authors.initials: J
authors.lastname: Havel
authors.forename: W
authors.initials: W
authors.lastname: Reineke
mesh_terms: D001140:Aroclors / Q000378:metabolism; D001565:Benzoates / Q000378:metabolism; D001673:Biodegradation, Environmental; D001713:Biphenyl Compounds / Q000378:metabolism*; D002396:Catechols / Q000378:metabolism; D006824:Hybridization, Genetic; D007700:Kinetics; D011549:Pseudomonas / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D001140:Aroclors; D001565:Benzoates; D001713:Biphenyl Compounds; D002396:Catechols; C032739:aroclor 1221; C055142:2-chlorobiphenyl; C006983:4-chlorobiphenyl; C034280:3-chlorobiphenyl