title: Isolation and characterization of a cDNA clone corresponding to the mouse t-complex gene Tcp-1x.
abstract: The mouse t complex on chromosome 17 is known to harbour many genes which have an important role in spermatogenesis. One of these, Tcp-1 has been cloned and shown to code for a protein probably essential for acrosome formation. During the isolation of a cDNA for Tcp-1 two other homologous sequences were recognized and described as Tcp-1x and Tcp-1y. In this paper we describe the isolation of a cDNA which has been shown by in situ hybridization to correspond to the Tcp-1x gene. Sequence analysis has confirmed that a 140 bp region of homology between Tcp-1 and Tcp-1x lies in the 3' portion of both genes. Northern blotting has revealed that the Tcp-1x gene is expressed abundantly in liver where two transcripts are detectable and hybrid selection shows that the gene codes for a 37 kDa protein. A search of the DNA database has failed to find any significant homology between Tcp-1x and any other sequences apart from Tcp-1.
authors.affiliation: Division of Biomolecular Sciences, King's College, Kensington.
authors.forename: K
authors.initials: K
authors.lastname: Dudley
authors.forename: F
authors.initials: F
authors.lastname: Shanahan
authors.forename: M
authors.initials: M
authors.lastname: Burtenshaw
authors.forename: E P
authors.initials: EP
authors.lastname: Evans
authors.forename: S
authors.initials: S
authors.lastname: Ruddy
authors.forename: M F
authors.initials: MF
authors.lastname: Lyon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D008869:Microtubule-Associated Proteins; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013091:Spermatogenesis / Q000235:genetics*; D044767:Ubiquitin-Protein Ligases; D056427:t-Complex Genome Region
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D004247:DNA; C116196:PPP1R11 protein, human; D044767:Ubiquitin-Protein Ligases