title: Elimination of V beta 2 bearing T-cells in BALB/c mice implanted with syngeneic preneoplastic and neoplastic mammary lesions.
abstract: The effect of growth of BALB/c C4 preneoplastic and neoplastic mammary lesions on the host T-cell repertoire was investigated. T-cells with specific V beta regions (V beta 2, -6, -8.1-2, -11, and -14) in the T-cell receptors were enumerated in C4 hyperplastic alveolar nodule (HAN)-infiltrating lymphocytes and lymph node cells from both C4 HAN and C4 tumor-bearing mice by flow cytometry with V beta-specific monoclonal antibodies. Growth of C4 HAN and tumor induced selective deletion of peripheral V beta 2+ T-cells. Elimination of V beta 2+ T-cells [7.2 +/- 0.8% (SD) of normal lymph node T-cells] by C4 HAN was biphasic and irreversible. Approximately one-half of the V beta 2+ T-cells were lost within the first month of C4 HAN implantation. Further reduction of V beta 2+ T-cells took place after another 3 months, at which time the level of V beta 2 was reduced to 1.2 +/- 0.1% of the total T-cell population. V beta 2 deletion occurred in BALB/c mice which had been implanted with C4 HAN at either 3 weeks or 2 months of age. Loss of V beta 2+ T-cells was not reversed by removal of C4 HAN. C4 tumor also induced V beta 2+ T-cell deletion. These results demonstrate a novel V beta 2 deleting activity expressed by C4 mammary lesions and suggest that during mouse mammary tumorigenesis, a unique "superantigen" is expressed which can cause profound systemic changes in the T-cell repertoire.
authors.affiliation: Department of Immunology, Michigan Cancer Foundation, Detroit 48201.
authors.forename: W Z
authors.initials: WZ
authors.lastname: Wei
authors.forename: R
authors.initials: R
authors.lastname: Ficsor-Jacobs
authors.forename: S J
authors.initials: SJ
authors.lastname: Tsai
authors.forename: R
authors.initials: R
authors.lastname: Pauley
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D005434:Flow Cytometry; D008212:Lymphocyte Depletion; D008325:Mammary Neoplasms, Experimental / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D011230:Precancerous Conditions / Q000276:immunology* / Q000473:pathology; D011948:Receptors, Antigen, T-Cell / Q000032:analysis / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014185:Transplantation, Isogeneic
chemical_list: D000911:Antibodies, Monoclonal; D011948:Receptors, Antigen, T-Cell