Crystallization and characterization of the high molecular weight form of nerve growth factor (7 S NGF).
A high molecular weight form of nerve growth factor (7 S NGF) has been crystallized in two crystal forms from polyethylene glycol 4000 by the vapour diffusion technique. The orthorhombic form A belongs to the space group P2(1)2(1)2(1) and has cell dimensions of a = 95.6, b = 96.5 and c = 147.0 A. With synchrotron X-ray radiation, these crystals diffract to 2.8 A resolution. They contain an intact 7 S NGF complex in the asymmetric unit. The tetragonal form B, which grows at similar conditions to the A form, belongs to the space group P4(1)2(1)2 (or P4(3)2(1)2) with unit cell dimensions of a = 97.4, b = 97.4 and c = 308.3 A. These crystals diffract to 3.6 A resolution and contain one 7 S complex per asymmetric unit. Native X-ray data have been collected to 3.3 A for the A form and to 5.0 A for the B form, both using synchrotron radiation.
McDonald
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Department of Crystallography, Birkbeck College, London, U.K.
Blundell
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TL
D000818:Animals; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D051379:Mice; D015394:Molecular Structure; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000737:chemistry*; D013363:Submandibular Gland / Q000737:chemistry; D014961:X-Ray Diffraction
D009414:Nerve Growth Factors