Novel meroterpenoids from Cystoseira mediterranea: use of the crown-gall bioassay as a primary screen for lipophilic antineoplastic agents.
Using a slight modification of the crown-gall potato disc bioassay, we were able to apply this test for two previously described antineoplastic lipophilic metabolites, didemnin B and mediterraneol A [1], and to use it as a guide for chromatographic separations of meroterpenoids from Cystoseira mediterranea. An active compound, mediterraneone [3], was isolated, and its structure was found to be a novel norsesquiterpenoid by chemical and spectral methods.
Fadli
M
M
Laboratoire de Chimie Marine (GERAP), Université de Perpignan, France.
Aracil
J M
JM
Jeanty
G
G
Banaigs
B
B
Francisco
C
C
D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification; D047630:Depsipeptides; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D008055:Lipids / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000459:Phaeophyceae / Q000032:analysis*; D011198:Solanum tuberosum; D013729:Terpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
D000970:Antineoplastic Agents; D047630:Depsipeptides; D008055:Lipids; D010456:Peptides, Cyclic; D013729:Terpenes; C069235:mediterraneone; C030051:didemnins; C069234:mediterraneol A