31P NMR characterization of terminal phosphates induced on DNA by the artificial nuclease 'Mn-TMPyP/KHSO5' in comparison with DNases I and II.
Phosphorus-31 NMR has been applied to the characterization of terminal phosphates on fragments of calf thymus DNA induced by three different nuclease systems: DNase I, DNase II and the artificial nuclease 'Mn-TMPyP/KHSO5'. In this last case, the oxidative damage to deoxyribose leads to two monophosphates esters (at the 3' and 5' ends) on both sides of the cleavage site. This method constitutes a promising approach to visualise the phosphate termini generated in DNA or RNA cleavage by cytotoxic drugs or chemical nucleases and provides a novel insight into the molecular aspects of their mechanism of action.
Gasmi
G
G
Laboratoire de Chimie de Coordination du CNRS, Toulouse, France.
Pasdeloup
M
M
Pratviel
G
G
Pitié
M
M
Bernadou
J
J
Meunier
B
B
D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D003850:Deoxyribonuclease I / Q000737:chemistry*; D004706:Endodeoxyribonucleases / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008345:Manganese / Q000737:chemistry*; D008665:Metalloporphyrins / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry*; D010761:Phosphorus Radioisotopes; D011188:Potassium; D017680:Potassium Compounds; D013431:Sulfates
D008665:Metalloporphyrins; D009943:Organophosphorus Compounds; D010761:Phosphorus Radioisotopes; D017680:Potassium Compounds; D013431:Sulfates; D008345:Manganese; C009007:potassium persulfate; C050950:tetrakis(N-methyl-4-pyridiniumyl)porphine manganese(III) complex; D004247:DNA; D004706:Endodeoxyribonucleases; D003850:Deoxyribonuclease I; C019329:deoxyribonuclease II; D011188:Potassium