N-myc oncogene expression in porcine renal development and oncogenesis.
N-myc oncogene expression was characterized in porcine kidneys to investigate the potential role of this gene in normal renal development and oncogenesis. N-myc RNA expression was detected in porcine kidneys from birth until 5 wk of age, which corresponds to the time when glomerular differentiation is completed. Immunohistochemical studies revealed that N-myc protein was selectively expressed in the primordia of renal proximal tubule epithelial cells. These cells were cultured in vitro and continued to express N-myc for a limited time. Comicroinjection of a mutant ras oncogene and N-myc into these cells led to focus formation in soft agar, loss of contact inhibition, and the establishment of an immortalized cell line. These findings support a multistep model of renal oncogenesis that involves overexpression of N-myc.
Bendit
I
I
Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas 75235-9063.
Rich
M A
MA
Moldwin
R
R
Waber
P
P
Steinberg
B M
BM
Nisen
P D
PD
D000818:Animals; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D016259:Genes, myc; D006801:Humans; D007668:Kidney / Q000254:growth & development* / Q000378:metabolism; D007680:Kidney Neoplasms / Q000235:genetics; D008969:Molecular Sequence Data; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D013552:Swine
D016271:Proto-Oncogene Proteins c-myc; D004247:DNA