Morphological and functional changes in Lewis lung carcinoma cells after treatment with A23187 calcium ionophore.
Cultured Lewis Lung carcinoma cells (3LL) treated with A23187 calcium ionophore were studied by transmission electron microscopy and HPLC analysis. Results showed that A23187 calcium ionophore induces on osmiophilic inclusion bodies of 3LL cells similar process of lamellar bodies formation to those reported in the development of the osmiophilic inclusions bodies within the granular pneumocyte of normal lungs. HPLC analysis shows that calcium ionophore generates quantitative changes in the 3LL cytoplasmic protein content expressed by an increase of 18-22 kDa and 4-9 kDa proteins.
Hilario
E
E
Department of Cell Biology and Morphological Sciences, Fac. of Medicine, University of Pais Vasco, Vizcaya, Spain.
Aliño
S F
SF
Barbero
M C
MC
Simon-Marin
R
R
García-Sanz
M
M
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology*; D003593:Cytoplasm / Q000737:chemistry; D008175:Lung Neoplasms / Q000473:pathology / Q000648:ultrastructure*; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis; D011506:Proteins / Q000032:analysis; D014407:Tumor Cells, Cultured / Q000187:drug effects
D011506:Proteins; D000001:Calcimycin