Preparation of soluble murine IL-6 receptor and anti-murine IL-6 receptor antibodies.
Starting with a previously isolated cDNA clone encoding murine IL-6R, a stable transformed Chinese hamster ovary cell line constitutively expressing soluble murine IL-6R (smIL-6R) has been established. The smIL-6R was purified to homogeneity by sequential filtration and chromatography of culture medium. The smIL-6R augmented the sensitivity of M1 cells to IL-6 in their growth inhibition in a dose-response manner. Rat hybridomas producing mAb specific to murine IL-6R were also established. One of the clones, RS13, produced IgG2a isotype that was capable of inhibiting IL-6 activity. ELISA for the quantitation of smIL-6R was established, which could detect smIL-6R in a quantity as low as 1 ng/ml.
Saito
T
T
Biotechnology Research Laboratory, Tosoh Corporation, Kanagawa, Japan.
Yasukawa
K
K
Suzuki
H
H
Futatsugi
K
K
Fukunaga
T
T
Yokomizo
C
C
Koishihara
Y
Y
Fukui
H
H
Ohsugi
Y
Y
Yawata
H
H
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D015850:Interleukin-6 / Q000378:metabolism / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D011971:Receptors, Immunologic / Q000032:analysis / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D019947:Receptors, Interleukin-6; D011994:Recombinant Proteins / Q000276:immunology; D012995:Solubility; D014162:Transfection
D000911:Antibodies, Monoclonal; D015850:Interleukin-6; D011971:Receptors, Immunologic; D019947:Receptors, Interleukin-6; D011994:Recombinant Proteins