Effect of reticuloendothelial blockade on tissue distribution of 99mTc-labeled synthetic liposomes in Ehrlich solid tumor-bearing mice.
The effect of a reticuloendothelial blockade was examined on the tissue distribution of 99mTc-labeled synthetic liposomes prepared from N,N-didodecyl-N alpha-[6-(trimethylammonio)hexanoyl]-L-alaninamide bromide (N+C5Ala2C12) in Ehrlich solid tumor-bearing mice. While a pre-dose of unlabeled phosphatidyl choline liposomes (natural liposomes) hardly influenced the tissue distribution of N+C5Ala2C12 liposomes, the pretreatment of dextran sulfate depressed the uptake in liver accompanied by increasing that in tumor and other tissues except the stomach. However, the extent of liver depression of N+C5Ala2C12 liposomes by dextran sulfate was lower than that of natural liposomes and the pre-dose of unlabeled natural liposomes had a minor effect on the tissue distribution of N+C5Ala2C12 liposomes compared with that of natural liposomes. In the liver uptake of N+C5Ala2C12 liposomes, it was suggested that Kupffer cell phagocytosis was not the main mechanism.
Goto
R
R
Department of Radiobiochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
Kubo
H
H
Okada
S
S
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D008081:Liposomes / Q000493:pharmacokinetics*; D051379:Mice; D012157:Mononuclear Phagocyte System / Q000378:metabolism*; D013667:Technetium; D014018:Tissue Distribution
D008081:Liposomes; D013667:Technetium