title: Production of the STA2-encoded glucoamylase in Saccharomyces cerevisiae is subject to feed-back control.
abstract: Three modes of production of the extracellular glucoamylase (GA) in Saccharomyces cerevisiae have been identified; repressed, basal and induced. The repressed mode is found with cells grown in rich media containing non-limiting concentrations of monosaccharides or disaccharides, including GA-hydrolysable maltose, as a sole carbon source. Both the basal and the induced modes (spanned by some seven-fold differences in the rate of GA production) can be displayed by either glucose-limited or glycerol-plus ethanol-consuming cultures; the induced mode is switched over to the basal one due to a feed-back inhibition by extracellularly accumulated GA. It is proposed that the feed-back control involved in GA production can be attenuated by starch which can thus 'induce' higher rates of GA production compared to the basal mode.
authors.affiliation: Institute of Genetics and Selection of Industrial Microorganisms, Moscow, USSR.
authors.forename: N I
authors.initials: NI
authors.lastname: Suntsov
authors.forename: S V
authors.initials: SV
authors.lastname: Kuchin
authors.forename: M A
authors.initials: MA
authors.lastname: Neystat
authors.forename: S V
authors.initials: SV
authors.lastname: Mashko
authors.forename: S V
authors.initials: SV
authors.lastname: Benevolensky
mesh_terms: D000688:Amylose / Q000378:metabolism; D004790:Enzyme Induction; D004794:Enzyme Repression; D000431:Ethanol / Q000378:metabolism; D005246:Feedback; D015966:Gene Expression Regulation, Fungal; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis* / Q000235:genetics; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D043324:beta-Fructofuranosidase
chemical_list: D000431:Ethanol; D000688:Amylose; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D005087:Glucan 1,4-alpha-Glucosidase; D005947:Glucose; D005990:Glycerol