[Purification and immunological behavior of beta-lactamase from Bacteroides fragilis].
The beta-lactamase crude extract of Bacteroides fragilis 55 was chromatographed with DEAE-sepharose CL-6B and sephadex G-100. The partial purified enzyme proteins was further purified by cutting the band on PAGE in which the beta-lactamase was distinguishable from other proteins by our method of fluorescent staining. Using purified preparations to be mixed with liposome-CPS-K, prepared specific antisera against the purified beta-lactamase. Serological reactions were carried out by IgG-ELISA together with western blotting. The results revealed that Bacteroides fragilis beta-lactamase possessed its species-specificity.
Chen
J
J
Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing.
Liu
B
B
Bao
Y
Y
D001441:Bacteroides fragilis / Q000201:enzymology*; D013045:Species Specificity; D001618:beta-Lactamases / Q000276:immunology / Q000302:isolation & purification*
D001618:beta-Lactamases