Bradykinin modulates the release of noradrenaline from vas deferens nerve terminals.
To asses whether bradykinin influences the release of noradrenaline from the adrenergic varicosities of the vas deferens, tissues were loaded with 3H-noradrenaline. Upon electrical depolarization bradykinin increased in a concentration-dependent fashion, the overflow of tritium from the mouse or rat vas deferens. The 3H-overflow is dependent on the external Ca2+concentration suggesting neuronal release of 3H-noradrenaline. The present results add evidence to the hypothesis that bradykinin modulates the release of noradrenaline from peripheral sympathetic nerve terminals via the activation of a presynaptic mechanism.
Llona
I
I
Laboratory of Biochemical Pharmacology, Faculty of Biological Sciences, Catholic University of Chile, Santiago.
Galleguillos
X
X
Belmar
J
J
Huidobro-Toro
J P
JP
D000818:Animals; D001920:Bradykinin / Q000032:analysis / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D008297:Male; D051379:Mice; D009638:Norepinephrine / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D011941:Receptors, Adrenergic / Q000378:metabolism; D014649:Vas Deferens / Q000294:innervation / Q000378:metabolism*
D011941:Receptors, Adrenergic; D001920:Bradykinin; D002118:Calcium; D009638:Norepinephrine