Phospholipase A activity in seminal plasma of men attending an infertility clinic.
Seminal plasma from 40 men attending an infertility clinic was analyzed for phospholipase A activity and compared with serum from 20 healthy blood donors. The imprecision of the method was acceptable and the overall coefficient of variation was 8.1%. A huge discrepancy existed between seminal plasma and blood serum with regard to phospholipase A activity, the former fluid displaying on average a 180-fold higher activity than the latter. Preincubation of seminal plasma for 1 h at 37 degrees C resulted in an even higher phospholipase A activity while the corresponding activity in serum remained unchanged by such a treatment. This suggested the existence of a zymogen form of phospholipase A in seminal plasma. A significant correlation existed between sperm concentration and phospholipase A activity in seminal plasma (r = 0.46; p less than 0.01) and preincubated seminal plasma samples from normozoospermic men displayed a significantly higher mean value than those of azoospermic men. Phospholipase A activity also correlated significantly to zinc concentration in seminal plasma (r = 0.67; p less than 0.001). 4-Bromophenacyl bromide was inhibitory to a certain extent, the inhibition being most evident in the samples with high phospholipase A activity. Dibucaine and quinacrine, known phospholipase inhibitors in other systems, had no inhibitory effects. Only 58% of the seminal plasma samples contained measurable amounts of triglycerides. No significant correlation existed between triglyceride concentration and phospholipase A activity, r = 0.11.
Jansson
A
A
Department of Clinical Chemistry, Uppsala University Hospital, Sweden.
Ronquist
G
G
Frithz
G
G
D000098:Acetophenones / Q000494:pharmacology; D003992:Dibucaine / Q000494:pharmacology; D006801:Humans; D007248:Infertility, Male / Q000201:enzymology*; D008297:Male; D009845:Oligospermia / Q000201:enzymology; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011796:Quinacrine / Q000494:pharmacology; D012661:Semen / Q000737:chemistry / Q000201:enzymology*; D013076:Sperm Count; D014280:Triglycerides / Q000032:analysis; D015032:Zinc / Q000032:analysis
D000098:Acetophenones; D014280:Triglycerides; D010741:Phospholipases A; D011796:Quinacrine; D015032:Zinc; D003992:Dibucaine; C006821:4-bromophenacyl bromide