[Histochemical investigations of the intracellular localization of adenylate cyclase activity after mitogenic or beta-adrenergic stimulation of T-lymphocytes of the mouse].
The role of AC at the beginning of an immune response is not fully understood until now. Using the specific substrate AMP-PNP we could show AC-activity in ConA-stimulated murine spleen lymphocytes histochemically. In contrast to beta-adrenergic stimulation (by Isoproterenol) ConA-induced AC-activity was found not only at the cell membrane and the nuclear membrane but also at some structures of the Golgi apparatus of the cells. The difference in AC-activity after beta-adrenergic or mitogenic stimulation is discussed.
Paukstat
D
D
Institut für Biochemie, Universität Rostock, Bundesrepublik Deutschland.
Schulze
W
W
Henkel
A
A
Hückel
C
C
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002451:Cell Compartmentation; D002462:Cell Membrane / Q000201:enzymology; D003208:Concanavalin A / Q000494:pharmacology; D006056:Golgi Apparatus / Q000201:enzymology; D006651:Histocytochemistry; D007545:Isoproterenol / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008808:Mice, Inbred CBA; D009685:Nuclear Envelope / Q000201:enzymology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D003208:Concanavalin A; D000262:Adenylyl Cyclases; D007545:Isoproterenol
Histochemische Untersuchungen zur intrazellulären Lokalisation von Adenylatzyklase-Aktivität nach mitogener bzw. beta-adrenerger Stimulierung von T-Lymphozyten der Maus.