title: Isolation and characterization of Streptomyces griseolus deletion mutants affected in cytochrome P-450-mediated herbicide metabolism.
abstract: Metabolism of sulfonylurea herbicides by Streptomyces griseolus ATCC 11796 is carried out via two cytochromes P-450, P-450SU1 and P-450SU2. Mutants of S. griseolus, selected by their reduced ability to metabolize a fluorescent sulfonylurea, do not synthesize cytochrome P-450SU1 when grown in the presence of sulfonylureas. Genetic evidence indicated that this phenotype was the result of a deletion of greater than 15 kb of DNA, including the structural genes for cytochrome P-450SU1 and an associated ferredoxin Fd-1 (suaC and suaB, respectively). In the absence of this monooxygenase system, the mutants described here respond to the presence of sulfonylureas or phenobarbital in the growth medium with the expression of only the subC,B gene products (cytochrome P-450SU2 and Fd-2), previously observed only as minor components in wild-type cells treated with sulfonylurea. These strains have enabled an analysis of sulfonylurea metabolism mediated by cytochrome P-450SU2 in the absence of P-450SU1, yielding an in vivo delineation of the roles of the two different cytochrome P-450 systems in herbicide metabolism by S. griseolus.
authors.affiliation: Central Research and Development Department, E.I. duPont de Nemours and Company, Wilmington, Delaware 19880.
authors.forename: P A
authors.initials: PA
authors.lastname: Harder
authors.forename: D P
authors.initials: DP
authors.lastname: O'Keefe
authors.forename: J A
authors.initials: JA
authors.lastname: Romesser
authors.forename: K J
authors.initials: KJ
authors.lastname: Leto
authors.forename: C A
authors.initials: CA
authors.lastname: Omer
mesh_terms: D015139:Blotting, Southern; D002851:Chromatography, High Pressure Liquid; D002872:Chromosome Deletion; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D005288:Ferredoxins / Q000378:metabolism; D006540:Herbicides / Q000378:metabolism*; D007700:Kinetics; D009154:Mutation; D013302:Streptomyces / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D013453:Sulfonylurea Compounds / Q000378:metabolism*
chemical_list: D005288:Ferredoxins; D006540:Herbicides; D013453:Sulfonylurea Compounds; D003577:Cytochrome P-450 Enzyme System