[Comparison of positivity between density gradient centrifugal method and whole blood method in lymphocyte surface marker analysis with flow-cytometry].
The lymphocyte surface marker analysis with flow-cytometry is usually performed using a density gradient centrifugal method or a whole blood method. We have compared these two methods using several monoclonal antibodies and have found the significant difference in positivity all monoclonal antibodies except TCR-1 (anti alpha/beta receptor on T-lymphocyte). We found the significantly higher positivity of Leu12 and HLA-DR as well as lower of the other monoclonal antibodies by using whole blood method than by density gradient centrifugal method. We conclude that it is necessary to make a distinct description of the method used in the surface marker analysis.
Bando
A
A
Department of Laboratory Medicine, Tokyo University Medical School.
Orita
T
T
Matsumoto
K
K
Yoneyama
A
A
Kitamura
K
K
Nakahara
K
K
Ohkubo
A
A
D000328:Adult; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface