title: Cloning of high-affinity methionine transport genes from Salmonella typhimurium.
abstract: At least one of the genes encoding a methionine transport system in Salmonella typhimurium has been cloned on a 15 kbp fragment in the lambda 1059 vector, and the region responsible for the transport activity was subcloned in a set of plasmids with inserts ranging from 1.4 to 4.2 kbp in the multicopy plasmid pUC8. Two proteins of Mr 34 and 40 kDa were expressed from the larger inserts.
authors.affiliation: Department of Applied Biology, University of Hull, U.K.
authors.forename: N A
authors.initials: NA
authors.lastname: Shaw
authors.forename: P D
authors.initials: PD
authors.lastname: Ayling
mesh_terms: D001692:Biological Transport / Q000235:genetics; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D008715:Methionine / Q000378:metabolism*; D015183:Restriction Mapping; D012486:Salmonella typhimurium / Q000235:genetics*; D014169:Transformation, Bacterial
chemical_list: D008715:Methionine