Cholesterol-rich LDL perfused at physiological LDL-cholesterol concentration induces platelet aggregation and PAF-acetylhydrolase activation.
The aim of this research was to perform an in vivo study on the relationships between lipid oxide (LP), platelet aggregation and PAF-acetylhydrolase in a model using perfusion of cholesterol-rich LDL media diluted to physiological LDL-cholesterol concentration. Normal rabbits were infused with LDL (d 1.025-1.063 g/ml) extracted from rabbits previously fed either with standard food (I-LDL group), 1% cholesterol food (II-LDL group) or 1% cholesterol plus probucol (IV-LDL group). CU2+ modified II-LDL was also infused (III-LDL group). After dilution as above, LP increased significantly in III- and II-LDL media. After perfusion, LP significantly increased in III- and II-LDL groups as compared to baseline values and to control. Compared to the I-LDL group, PAF-acetylhydrolase and platelet aggregation significantly increased in III- and II-LDL groups. These data indicate the property of cholesterol- rich LDL to activate PAF-acetylhydrolase and enhance platelet aggregation, even when perfused through a medium containing a physiological LDL-cholesterol concentration.
Chui
D H
DH
Department of Neurology, Norman Bethune University of Medical Sciences, Chagchun, China.
Marotta
F
F
Rao
M L
ML
Liu
D S
DS
Zhang
S C
SC
Ideo
C
C
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D000818:Animals; D008078:Cholesterol, LDL / Q000008:administration & dosage / Q000494:pharmacology*; D008074:Lipoproteins / Q000097:blood; D010477:Perfusion; D010741:Phospholipases A / Q000378:metabolism*; D010974:Platelet Aggregation / Q000187:drug effects*; D011817:Rabbits
D008078:Cholesterol, LDL; D008074:Lipoproteins; D010741:Phospholipases A; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase