Inhibition of intercellular communication by airborne particulate matter.
To investigate the inhibition of gap junction mediated intercellular communication (IC) by extracts of airborne particulate matter (APM), V79 cells were incubated with extracts of APM and subsequently microinjected with the fluorescent dye Lucifer Yellow, after which the number of fluorescent (= communicating) cells was determined. To compare inhibitory effects on IC with mutagenicity, APM was also tested in the Salmonella microsome assay. Six different extracts were tested, two outdoor extracts representing a heavily polluted and a relatively clean sample, and four indoor extracts, taken either in livingrooms with or without wood combustion in an open fire place, or in a room with or without cigarette smoking. Non-cytotoxic doses of outdoor and indoor APM inhibited IC in V79 cells in a dose- and time-dependent manner. Mutagenicity data and IC data were correlated. These results suggest that APM has tumor promoter activity in addition to mutagenic activity.
Heussen
G A
GA
Department of Toxicology, Agricultural University, Wageningen, The Netherlands.
D000393:Air Pollutants / Q000633:toxicity*; D002450:Cell Communication / Q000187:drug effects*; D002460:Cell Line; D009153:Mutagens; D011493:Protein Kinase C / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000393:Air Pollutants; D009153:Mutagens; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate