Inhibition of duck hepatitis B virus infection by lysosomotropic agents.
The early phases of hepadnaviral infection were studied in primary duck hepatocyte cultures. Incubation of duck hepatocytes in vitro with duck hepatitis B virus (DHBV) resulted in infection with high levels of viral replication. The lysosomotropic agents ammonium chloride and chloroquine effectively inhibited viral infection, indicating that DHBV infection, similar to infection with other enveloped viruses, depends on receptor-mediated endocytosis and involves membrane fusion triggered by low pH.
Offensperger
W B
WB
Department of Medicine, University of Freiburg, Germany.
Offensperger
S
S
Walter
E
E
Blum
H E
HE
Gerok
W
W
D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D002478:Cells, Cultured; D002738:Chloroquine / Q000494:pharmacology; D004372:Ducks; D015611:Hepatitis B Virus, Duck / Q000187:drug effects* / Q000254:growth & development; D006524:Hepatitis, Viral, Animal / Q000517:prevention & control*; D008247:Lysosomes / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D000643:Ammonium Chloride; D002738:Chloroquine