A gene structure of testosterone 6 beta-hydroxylase (P450IIIA).
Genomic clones of a rat testosterone 6 beta-hydroxylase have been isolated and characterized as the first gene (P450/6 beta A) among P450IIIA subfamily. This gene spans about 25Kb and consists of 13 exons, which is the largest number of exons among cytochrome P-450 genes reported previously. The nucleotide sequence of the exon region showed high similarity to those of P450PCN2 and P450PCN1 cDNA (Gonzalez, F.J. et al. (1987) Mol. Cell. Biol. 2969-2974), but several replacements and deletions of nucleotide were found between the P450/6 beta A gene and both cDNAs, indicating the existence of multiple P450IIIA genes in rats.
Miyata
M
M
Department of Pharmacology, School of Medicine, Keio University, Tokyo, Japan.
Nagata
K
K
Yamazoe
Y
Y
Kato
R
R
D000818:Animals; D001483:Base Sequence; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005091:Exons; D005796:Genes; D015698:Genomic Library; D007438:Introns; D008969:Molecular Sequence Data; D005810:Multigene Family; D015345:Oligonucleotide Probes; D051381:Rats; D011919:Rats, Inbred Strains; D015183:Restriction Mapping; D013250:Steroid Hydroxylases / Q000235:genetics*; D016385:TATA Box; D014158:Transcription, Genetic
D015345:Oligonucleotide Probes; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; C022657:steroid hormone 6-beta-hydroxylase