Cisplatin-induced injury to calcium uptake by mitochondria in glutathione-depleted slices of rat kidney cortex.
Changes in functional activities of mitochondria from kidney cortical slices incubated with cisplatin and the glutathione depletor diethylmaleate were examined. Diethylmaleate, which decreased the glutathione level in the slices, enhanced the cisplatin-induced decreases in glutathione level and calcium uptake in the mitochondria. The movement of cisplatin into mitochondria in the slices was not affected by diethylmaleate. These results suggest that the depressions in glutathione level and calcium uptake by cisplatin in mitochondria are enhanced by a decrease in cytoplasmic glutathione.
Kameyama
Y
Y
Division of Pharmacology, Osaka University of Pharmaceutical Sciences, Japan.
Gemba
M
M
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002945:Cisplatin / Q000633:toxicity*; D004357:Drug Synergism; D005978:Glutathione / Q000502:physiology*; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008298:Maleates / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D008298:Maleates; C014476:diethyl maleate; D005978:Glutathione; D002945:Cisplatin; D002118:Calcium