title: Methodological problems of direct bioluminescent ADP assay in platelets and erythrocytes.
abstract: We have developed a method for ADP bioluminescent measurement in platelets and erythrocytes which complements our previous method for ATP assay. When the different parameters of the system under investigation are taken into account, a linea range between 10(-9) and 10(-7) g/ml can be obtained without incubation or troublesome extraction. This makes the method easy and useful for identifying any disease-induced alterations in ATP and/or ADP levels in these blood cells. The data obtained correlate well with those of a bioluminescent method requiring extraction with ethanol/EDTA and incubation, giving the reference intervals of 3.5-5.5 mumol/10(11) PLT for ATP determination and 1.9-3.7 mumol/10(11) PLT for ADP determination in platelets, and 3.2-3.8 mumol/g Hgb for ATP determination and 0.56-0.73 mumol/g Hgb for ADP in erythrocytes. This assay was applied to quality control on blood bags in transfusion centers and proved to be a rapid and reliable method for testing the viability of stored blood cells.
authors.affiliation: Istituto di Scienze Chimiche, Università di Bologna, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Girotti
authors.forename: E
authors.initials: E
authors.lastname: Ferri
authors.forename: M L
authors.initials: ML
authors.lastname: Cascione
authors.forename: A
authors.initials: A
authors.lastname: Orlandini
authors.forename: L
authors.initials: L
authors.lastname: Farina
authors.forename: S
authors.initials: S
authors.lastname: Nucci
authors.forename: F
authors.initials: F
authors.lastname: Di Graci
authors.forename: R
authors.initials: R
authors.lastname: Budini
mesh_terms: D000244:Adenosine Diphosphate / Q000032:analysis*; D000255:Adenosine Triphosphate / Q000032:analysis; D001792:Blood Platelets / Q000737:chemistry*; D001793:Blood Preservation; D004912:Erythrocytes / Q000737:chemistry*; D005615:Freezing; D006801:Humans; D008163:Luminescent Measurements; D008722:Methods
chemical_list: D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate