Determination of a new 2-amino-2-oxazoline (COR 3224) in plasma and brain tissue of the rat by high-performance liquid chromatography with electrochemical detection.
A reversed-phase (CN as stationary phase) liquid chromatographic method with electrochemical detection is described for the quantitation of COR 3224, a new 2-amino-2-oxazoline in plasma and brain tissue of the rat. Extraction was performed with dichloromethane and detection was achieved at a working electrode potential of +0.85 V versus an Ag/AgCl reference electrode. The recovery of the method is about 80 and 60% for plasma and brain, respectively. The limit of detection was less than 10 ng/ml for both plasma and brain, five times lower than that with ultraviolet detection.
Damaj
M I
MI
Laboratoire de Pharmacologie et de Pharmacocinétique, Faculté de Pharmacie, Chatenay-Malabry, France.
Trouvin
J H
JH
Lambrey
B
B
Jacquot
C
C
D000818:Animals; D000928:Antidepressive Agents / Q000032:analysis* / Q000097:blood; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D010080:Oxazoles / Q000032:analysis* / Q000097:blood; D051381:Rats; D014466:Ultraviolet Rays
D000928:Antidepressive Agents; D010080:Oxazoles; C056984:COR 32-24