Properties of the streptomycete temperate bacteriophage FP43.
FP43 is a temperate bacteriophage for Streptomyces griseofuscus that forms plaques on many Streptomyces species. FP43 virions contain 56 kb of double-strand DNA that is circularly permuted and terminally redundant, and contains 65% G + C. A physical map of the FP43 genome was constructed, and the origin for headful packaging (pac) was localized to an 8.8-kb region of the genome (hft) that mediates high-frequency transduction by FP43 of plasmid pRHB101. The phage attachment site (attP), a replication origin (rep), a region that inhibits plaque formation (pin), and a 3-kb deletion (rpt) that caused a 100-fold reduction in plasmid transduction were mapped.
Hahn
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Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285.
McHenney
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Baltz
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D001435:Bacteriophages / Q000235:genetics* / Q000502:physiology; D015139:Blotting, Southern; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D010957:Plasmids; D015183:Restriction Mapping; D013302:Streptomyces; D014161:Transduction, Genetic; D010948:Viral Plaque Assay; D014771:Virion / Q000235:genetics
D004279:DNA, Viral