Antagonism of kinin-induced contraction of isolated rat uterus by the crude hydroalcoholic extract from Mandevilla illustris.
1. The crude aqueous/alcoholic extract (CE) of Mandevilla illustris (Apocynaceae) rhizomes was analysed against contractile response elicited by bradykinin (BK), lysyl-bradykinin (L-BK), oxytocin(Ot), acetylcholine (Ach), angiotensin II (AII) and barium chloride (BaCl2) in the isolated uterus of the rat. 2. The CE of this plant (0.5-2.0 mg/ml) caused a parallel and concentration-related rightward displacement of BK and L-BK contractile responses. Schild plot revealed a linear relationship (r close to one) and yielded nominal PA2 values of 3.6 and 3.2 respectively, but the slopes were significantly different from unity. 3. However, the anti-BK action of the CE of M. illustris was not selective to kinin action, since in the same range concentration the CE also affected uterine contractile responses induced by Ot, Ach, AII and BaCl2.
Calixto
J B
JB
Department of Pharmacology, Universidade Federal de Santa Catarina, Florianópolis, Brazil.
Yunes
R A
RA
D000109:Acetylcholine / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000804:Angiotensin II / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000818:Animals; D001464:Barium / Q000037:antagonists & inhibitors / Q000494:pharmacology; D017609:Barium Compounds; D001920:Bradykinin / Q000037:antagonists & inhibitors / Q000494:pharmacology; D002712:Chlorides; D000431:Ethanol; D005260:Female; D066298:In Vitro Techniques; D007705:Kinins / Q000037:antagonists & inhibitors*; D010121:Oxytocin / Q000037:antagonists & inhibitors / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D014590:Uterine Contraction / Q000187:drug effects*; D014867:Water
D017609:Barium Compounds; D002712:Chlorides; D007705:Kinins; D010936:Plant Extracts; D014867:Water; C024986:barium chloride; D000804:Angiotensin II; D001464:Barium; D000431:Ethanol; D010121:Oxytocin; D000109:Acetylcholine; D001920:Bradykinin