title: Purification of Food Color Red No. 106 (acid red) using high-speed counter-current chromatography.
abstract: High-speed counter-current chromatography (HSCCC) has been successfully applied to the separation of the components of Food Color Red No. 106 (R-106). The separation was performed using 25 mg of the sample with a two-phase solvent system composed of n-butanol and 0.01 M trifluoroacetic acid (1:1, v/v). Analyses by thin-layer chromatography, high-performance liquid chromatography and fast atom bombardment mass spectrometry confirmed that HSCCC was effective in the purification of the components of R-106. The separation gave 21 mg of a 99.9% pure main component (Acid Red) and 0.9 mg of 98.0% pure subsidiary dye which is probably a des-ethyl derivative.
authors.affiliation: Aichi Prefectural Institute of Public Health, Nagoya, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Oka
authors.forename: Y
authors.initials: Y
authors.lastname: Ikai
authors.forename: N
authors.initials: N
authors.lastname: Kawamura
authors.forename: J
authors.initials: J
authors.lastname: Hayakawa
authors.forename: M
authors.initials: M
authors.lastname: Yamada
authors.forename: K
authors.initials: K
authors.lastname: Harada
authors.forename: H
authors.initials: H
authors.lastname: Murata
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Nakazawa
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
mesh_terms: D001391:Azo Compounds / Q000302:isolation & purification*; D002498:Centrifugation; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D002855:Chromatography, Thin Layer; D005505:Food Coloring Agents / Q000302:isolation & purification*; D009282:Naphthalenesulfonates / Q000302:isolation & purification*; D012995:Solubility; D012997:Solvents; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D001391:Azo Compounds; D005505:Food Coloring Agents; D009282:Naphthalenesulfonates; D012997:Solvents; C069309:C.I. Acid Red 106