title: Co-amplification of L1 line elements with localised low copy repeats in Giemsa dark bands: implications for genome organisation.
abstract: A repeat sequence island, located at the A3 Giemsa dark band on the mouse X chromosome and consisting of 50 copies of a localised long complex repeat unit (LCRU), features an unusually high concentration of L1 LINE repeat sequences juxtaposed and inserted within the LCRU. Sequence analysis of three independent genomic clones containing L1 LINE elements juxtaposed with the LCRU demonstrates a common junction sequence at the L1/LCRU boundary, suggesting that the high concentration of L1 LINE sequences in the repeat sequence island has arisen by association of an L1 element with an LCRU followed by amplification. The LCRU target site at this common junction sequence bears no resemblance to the target site of an L1 element inserted within one LCRU, indicating there is no specific preferential target site for L1 integration. We propose that co-amplification of L1 LINE elements with localised low copy repeat families throughout the genome could have a major effect on the chromosomal distribution of L1 LINE elements.
authors.affiliation: Department of Biochemistry and Molecular Genetics, St Mary's Hospital Medical School, London, UK.
authors.forename: J
authors.initials: J
authors.lastname: Nasir
authors.forename: M K
authors.initials: MK
authors.lastname: Maconochie
authors.forename: S D
authors.initials: SD
authors.lastname: Brown
mesh_terms: D000818:Animals; D001483:Base Sequence; D002871:Chromosome Banding; D003001:Cloning, Molecular; D004247:DNA; D004276:DNA, Satellite / Q000737:chemistry; D005784:Gene Amplification; D051379:Mice; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014960:X Chromosome
chemical_list: D004276:DNA, Satellite; D004247:DNA