Increase of O6-methylguanine-DNA-methyltransferase and N3-methyladenine glycosylase RNA transcripts in rat hepatoma cells treated with DNA-damaging agents.
A variety of DNA-damaging agents increase the O6-methylguanine-DNA-methyltransferase (transferase) and the N3-methyladenine (3-meAde)-DNA-glycosylase activities in a rat hepatoma cell line (H4 cells). Using two cDNA expressing either the rat 3-meAde-DNA-glycosylase or the transferase, the level of mRNA transcripts was measured by hybridization in H4 cells treated with three different inducing agents, gamma-rays, cis-dichlorodiammine platinum II or N-methyl-9-hydroxy ellipticinium. The two mRNA increased 24 hours after the cell treatments but this enhanced transcription was a transient phenomenon, as it was no longer observed after 96 hours. No significant DNA amplification was detectable in the treated cells.
Laval
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U247 INSERM, Institut Gustave Roussy, Villejuif, France.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D002945:Cisplatin / Q000494:pharmacology*; D004249:DNA Damage; D045647:DNA Glycosylases; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects; D004611:Ellipticines / Q000494:pharmacology*; D029968:Escherichia coli Proteins; D005720:Gamma Rays; D008114:Liver Neoplasms, Experimental / Q000201:enzymology; D009699:N-Glycosyl Hydrolases / Q000235:genetics* / Q000378:metabolism; D019853:O(6)-Methylguanine-DNA Methyltransferase; D051381:Rats; D014157:Transcription Factors; D014158:Transcription, Genetic / Q000187:drug effects* / Q000528:radiation effects
D000970:Antineoplastic Agents; D001426:Bacterial Proteins; D004611:Ellipticines; D029968:Escherichia coli Proteins; D014157:Transcription Factors; C027767:elliptinium; C045675:Ada protein, E coli; D019853:O(6)-Methylguanine-DNA Methyltransferase; C021565:3-methyladenine-DNA glycosylase; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D002945:Cisplatin