Physiology of bone: mineral compartment proteins as candidates for environmental perturbation by lead.
Termine et al. first demonstrated that sequential dissociative extraction and fractionation procedures with protease inhibitors could provide a convenient approach for the study of mineral compartment constituents. The primary extraction regimen used 4 M guanidine HCl to remove most of the protein from the nonmineralized phase of bone. Subsequently, EDTA-guanidine was used to remove the mineral-phase components. These methods discriminate on the basis of physical-chemical association with a mineral phase rather than on the specific gene products of a particular cell. In the present discussion emphasis is directed at a group of divalent cation binding proteins isolated from the mineral compartment of bone. The localization, synthesis, and chemical characteristics of osteonectin, bone sialoproteins I and II, and bone acidic glycoprotein-75 are discussed and offered as possible sites for perturbation by the environment with lead exposure.
Sauk
J J
JJ
Department of Pathology, University of Maryland Dental School, Baltimore 21201.
Somerman
M J
MJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D001857:Bone Matrix / Q000187:drug effects*; D004785:Environmental Pollutants / Q000506:poisoning*; D006706:Homeostasis; D006801:Humans; D007855:Lead Poisoning / Q000378:metabolism*; D008903:Minerals / Q000378:metabolism*; D008969:Molecular Sequence Data; D015676:Osteonectin / Q000187:drug effects; D011506:Proteins / Q000187:drug effects*
D004785:Environmental Pollutants; D008903:Minerals; D015676:Osteonectin; D011506:Proteins