title: Primary structure of the large (L) RNA segment of nephropathia epidemica virus strain Hällnäs B1 coding for the viral RNA polymerase.
abstract: The L RNA segment of the nephropathia epidemica virus (NEV) strain Hällnäs B1 was characterized by molecular cloning of the corresponding cDNA and subsequent determination of the DNA nucleotide sequence. The L RNA segment is 6550 nucleotides long with complementarity of 20 bases at the 3' and 5' termini. The viral messenger sense RNA contains one major open reading frame (ORF) with a coding capacity of 2156 amino acid residues encoding a protein with a calculated molecular weight of 246 kDa and an IEP of pH 7.4. Comparison of the deduced amino acid sequences from NEV hantavirus and Bunyamwera virus (BWV) L segment messenger sense RNAs, revealed a high degree of diversity (overall amino acid identity, 17%). However, three clusters of 30-40% amino acid identity were detected. One of these domains, containing an Asp-Asp motif found in many RNA polymerases, also shares amino acid sequence homology with the PB1 polymerase component of influenza type A. These results indicate that the L RNA segment of the NEV codes for the viral RNA-dependent RNA polymerase. The data presented here complete our previous studies on the characterization of the NEV genome by cDNA sequencing of the viral M and S RNA segments.
authors.affiliation: ZMBH, Heidelberg, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Stohwasser
authors.forename: K
authors.initials: K
authors.lastname: Raab
authors.forename: G
authors.initials: G
authors.lastname: Darai
authors.forename: E K
authors.initials: EK
authors.lastname: Bautz
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002043:Bunyaviridae / Q000201:enzymology / Q000235:genetics*; D004279:DNA, Viral / Q000737:chemistry*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012367:RNA, Viral / Q000737:chemistry*; D012324:RNA-Dependent RNA Polymerase / Q000096:biosynthesis / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014709:Vero Cells
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase