title: Paramyosin is the Schistosoma mansoni (Trematoda) homologue of antigen B from Taenia solium (Cestoda).
abstract: Antigen B, a major antigen of the cestode parasite Taenia solium, has been purified and a portion of amino acid sequence obtained. Paramyosin of the trematode parasite Schistosoma mansoni, an immunogenic protein that has shown promise as a vaccine candidate, has several biochemical and immunological properties in common with antigen B. A full-length cDNA clone of S. mansoni paramyosin has been obtained and the predicted translation product contains a sequence that is highly homologous to the sequence obtained for antigen B. The predicted amino acid composition and isolectric point of paramyosin are nearly identical to those established for antigen B. Recombinant S. mansoni paramyosin, expressed in Escherichia coli as a fusion protein with beta-galactosidase, was recognized by antisera against T. solium antigen B. We conclude from these results that S. mansoni paramyosin and T. solium antigen B are homologous proteins. Since S. mansoni paramyosin is thought to be a muscle protein and T. solium antigen B a secreted glycoprotein with anti-complement activity, this conclusion raises some interesting questions regarding the role of this class of proteins in the host-parasite relationship.
authors.affiliation: Department of Immunology, Universidad Nacional Autónoma de México, Mexico City.
authors.forename: J P
authors.initials: JP
authors.lastname: Laclette
authors.forename: A
authors.initials: A
authors.lastname: Landa
authors.forename: L
authors.initials: L
authors.lastname: Arcos
authors.forename: K
authors.initials: K
authors.lastname: Willms
authors.forename: A E
authors.initials: AE
authors.lastname: Davis
authors.forename: C B
authors.initials: CB
authors.lastname: Shoemaker
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D004247:DNA; D015870:Gene Expression; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D015183:Restriction Mapping; D012550:Schistosoma mansoni / Q000235:genetics* / Q000276:immunology; D012689:Sequence Homology, Nucleic Acid; D013621:Taenia / Q000235:genetics* / Q000276:immunology; D014335:Tropomyosin / Q000235:genetics* / Q000276:immunology
chemical_list: D000947:Antigens, Helminth; D014335:Tropomyosin; D004247:DNA