Dopamine activation of an orphan of the steroid receptor superfamily.
The chicken ovalbumin upstream promoter transcription factor (COUP-TF) is a member of the steroid receptor superfamily and participates in the regulation of several genes. While a number of functions have been ascribed to COUP-TF, no ligand or activator molecule has been identified, and thus it is classified as one of a group of orphan receptors. Activation of COUP-TF by physiological concentrations of the neurotransmitter dopamine was observed in transient transfection assays. Treatment of transfected cells with the dopamine receptor agonist alpha-ergocryptine also activated COUP-dependent expression of a reporter gene. COUP-TF that contained a deletion in the COOH-terminal domain was not activated by these compounds. These observations suggest that dopamine may be a physiological activator of COUP-TF.
Power
R F
RF
Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030.
Lydon
J P
JP
Conneely
O M
OM
O'Malley
B W
BW
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D002645:Chickens; D002678:Chimera; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D004298:Dopamine / Q000494:pharmacology*; D004873:Ergolines / Q000494:pharmacology; D004988:Ethers, Cyclic / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D019319:Okadaic Acid; D010047:Ovalbumin / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011987:Receptors, Steroid / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D014162:Transfection
D004873:Ergolines; D004988:Ethers, Cyclic; D011987:Receptors, Steroid; D014157:Transcription Factors; D019319:Okadaic Acid; D015124:8-Bromo Cyclic Adenosine Monophosphate; C100269:ergocryptine; D010047:Ovalbumin; D015500:Chloramphenicol O-Acetyltransferase; D004298:Dopamine