title: Divalent cation-sensitive antagonist binding to heart muscarinic receptors.
abstract: 1. The binding of (-) [3H]quinuclidinylbenzilate (QNB), a potent muscarinic antagonist, to cardiac muscarinic receptors was examined in washed particles and microsomes isolated from rat heart atria. 2. Addition of divalent cations (Mg2+, Mn2+ and Ca2+) to the binding assays increased (6 to 8-fold) the antagonist binding to microsomes but exerted little or only weak (up to 35%) stimulation of the antagonist binding to washed particles. 3. Mg2+ ions present only in the dilution and washing buffer prior to rapid filtration of the binding reaction were capable of markedly increasing the antagonist binding to microsomes but not to washed particles. 4. Such differential action of Mg2+ on antagonist binding to subcellular fractions was seen when fractions were either isolated and or incubated in media containing either 10 or 50 mM imidazole-Cl, Tris-phosphate or Tris-Cl buffer, each at pH 7.5. While the modulatory effect of Mg2+ was not seen in Na-K-phosphate buffer, pH 7.5, the antagonist binding per se was much higher in Na-K-phosphate buffer compared to other buffers.
authors.affiliation: Department of Physiology, College of Medicine, University of Saskatchewan, Saskatoon, Canada.
authors.forename: P V
authors.initials: PV
authors.lastname: Sulakhe
authors.forename: G
authors.initials: G
authors.lastname: Jagadeesh
authors.forename: N T
authors.initials: NT
authors.lastname: Phan
mesh_terms: D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D002021:Buffers; D002118:Calcium / Q000494:pharmacology; D002413:Cations, Divalent / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008345:Manganese / Q000494:pharmacology; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D010276:Parasympatholytics / Q000378:metabolism*; D011813:Quinuclidinyl Benzilate; D051381:Rats; D011919:Rats, Inbred Strains; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000378:metabolism*; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism
chemical_list: D002021:Buffers; D002413:Cations, Divalent; D010276:Parasympatholytics; D011976:Receptors, Muscarinic; D008345:Manganese; D011813:Quinuclidinyl Benzilate; D008274:Magnesium; D002118:Calcium