title: A simplified method for screening the apolipoprotein E polymorphism.
abstract: A simple method for screening populations for the apolipoprotein E polymorphism which involves isoelectric focusing of delipidated samples on polyacrylamide gels of pH 4.5-5.8 followed by immunoblotting using a double-antibody technique is presented. The method is a synthesis of two previously published procedures and works well on samples that have been stored for as long as 15 years.
authors.affiliation: Department of Epidemiology, Graduate School of Public Health, University of Pittsburgh, Pa.
authors.forename: J E
authors.initials: JE
authors.lastname: Eichner
authors.forename: L H
authors.initials: LH
authors.lastname: Kuller
authors.forename: R E
authors.initials: RE
authors.lastname: Ferrell
authors.forename: M I
authors.initials: MI
authors.lastname: Kamboh
mesh_terms: D000328:Adult; D001057:Apolipoproteins E / Q000235:genetics*; D002318:Cardiovascular Diseases / Q000175:diagnosis / Q000235:genetics; D005260:Female; D005820:Genetic Testing; D006801:Humans; D007525:Isoelectric Focusing; D008137:Longitudinal Studies; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012307:Risk Factors
chemical_list: D001057:Apolipoproteins E