Stimulation of carnitine acetyltransferase in PC12 cells by nerve growth factor: relationship to choline acetyltransferase stimulation.
The activity of carnitine acetyltransferase (acetyl-CoA:L-carnitine O-acetyltransferase) was found to be at least 50-fold higher than that of choline acetyltransferase in PC12 cells. Nerve growth factor stimulated both enzymes in a parallel manner with respect to concentration of NGF and culture time. The stimulation of both enzymes was completely inhibited by 10 microM 6-thioguanine, an inhibitor of protein kinase N. Results are discussed with reference to the hypothesis that the two enzymes may be functionally related in neuronal cells.
White
H L
HL
Div. of Pharmacology, Wellcome Research Laboratories, Research Triangle Park, North Carolina 27709.
Scates
P W
PW
D000310:Adrenal Gland Neoplasms / Q000201:enzymology*; D000818:Animals; D002332:Carnitine O-Acetyltransferase / Q000378:metabolism*; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000494:pharmacology*; D010673:Pheochromocytoma / Q000201:enzymology*; D011493:Protein Kinase C; D047428:Protein Kinase Inhibitors; D051381:Rats; D013866:Thioguanine / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D009414:Nerve Growth Factors; D047428:Protein Kinase Inhibitors; D002795:Choline O-Acetyltransferase; D002332:Carnitine O-Acetyltransferase; C061570:protein kinase N; D011493:Protein Kinase C; D013866:Thioguanine