Determination of cyclohexanol in urine and its use in environmental monitoring of cyclohexanone exposure.
A simple and sensitive method for determining urinary cyclohexanol, the main metabolite of cyclohexanone, by hydrolysis and gas chromatography (GC) with a flame ionization detector was developed. A 2-mL urine sample was hydrolyzed with 0.4 mL of concentrated HCl and followed by extracting twice with diethylether. Two microL of the filtrate was injected into the GC with a methyl silicone column. The detection limit is estimated to be 0.4 mg/L. The coefficient of variation for the procedure is 8% and 10% for the range of concentration 5 and 50 mg/L, respectively. The within-run variation was 5.4% and between-day variation was 9.67%. The method was verified with urine samples collected from workers exposed to cyclohexanone. An excellent correlation (r = 0.88) was observed between environmental cyclohexanone exposure and cyclohexanol in urine. The procedure is relatively simple and reproducible and it can be applied for occupational health measurement of cyclohexanone exposure.
Ong
C N
CN
Department of Community, Occupational and Family Medicine, National University of Singapore.
Sia
G L
GL
Chia
S E
SE
Phoon
W H
WH
Tan
K T
KT
D002138:Calibration; D002849:Chromatography, Gas; D003511:Cyclohexanols / Q000652:urine*; D003512:Cyclohexanones / Q000652:urine*; D004784:Environmental Monitoring / Q000379:methods*; D006801:Humans; D006868:Hydrolysis; D016273:Occupational Exposure
D003511:Cyclohexanols; D003512:Cyclohexanones; C036468:cyclohexanone