Expression in monkey cells of the missing enzyme in L-ascorbic acid biosynthesis, L-gulono-gamma-lactone oxidase.
L-Gulono-gamma-lactone oxidase (GLO), an enzyme that is missing in scurvy-prone animals, was produced in COS-1 cells by transfection with a minigene constructed from pSVL vector and rat GLO cDNA. A functional GLO protein was expressed, which was indistinguishable from rat GLO in molecular size. The microsomes obtained from the transfected COS-1 cells showed GLO activity comparable to that in rat liver microsomes.
Yagi
K
K
Institute of Applied Biochemistry, Yagi Memorial Park, Japan.
Koshizaka
T
T
Kito
M
M
Ozawa
T
T
Nishikimi
M
M
D000818:Animals; D001205:Ascorbic Acid / Q000096:biosynthesis* / Q000235:genetics; D002460:Cell Line; D002522:Chlorocebus aethiops; D015870:Gene Expression; D005813:Genes, Synthetic; D007668:Kidney; D050544:L-Gulonolactone Oxidase; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis; D013401:Sugar Alcohol Dehydrogenases / Q000096:biosynthesis / Q000235:genetics*; D014162:Transfection
D011994:Recombinant Proteins; D013401:Sugar Alcohol Dehydrogenases; D050544:L-Gulonolactone Oxidase; D001205:Ascorbic Acid