Cloning of the murine lymphocyte function-associated molecule-1 alpha-subunit and its expression in COS cells.
The lymphocyte function-associated molecule 1 (LFA-1, CD11a/CD18) is an integrin that mediates adhesion of immune cells by interaction with two members of the Ig superfamily, ICAM-1 and ICAM-2. LFA-1 consists of an alpha subunit (Mr = 180,000) and a beta subunit (Mr = 95,000). We report here the isolation and expression of the murine alpha subunit cDNA (GenBank accession no. M60778). The deduced sequence comprises a 1061 amino acid extracellular domain, a 29 amino acid transmembrane region, and a 50 amino acid cytoplasmic domain. It has a 72% amino acid identity with its human counterpart and 34% identity with the murine Mac-1 alpha subunit. The murine LFA-1 alpha subunit could be expressed on the cell surface of a fibroblastoid cell line, COS, by cotransfection with either the human or murine beta subunit cDNA.
Kaufmann
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Y
Center for Blood Research, Harvard Medical School, Boston, MA 02115.
Tseng
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E
Springer
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TA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D016169:Lymphocyte Function-Associated Antigen-1 / Q000235:genetics*; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D013045:Species Specificity; D014162:Transfection
D016169:Lymphocyte Function-Associated Antigen-1; D046911:Macromolecular Substances; D004247:DNA