Seasonal effects of administration of melatonin and 5-methoxytryptophol on ovarian activity in the catfish Heteropneustes fossilis (Bloch).
In Heteropneustes fossilis, effects of intraperitoneal administration (25 micrograms/fish, alternate days for a month 1 hr before the onset of dark phase) of melatonin (MEL) and 5-methoxytryptophol (5-MTL) on ovarian activity were investigated during different seasons of the annual reproductive cycle under natural photothermal conditions. The gonadosomatic index (GSI) had decreased significantly by MEL during preparatory and prespawning seasons, and by 5-MTL in all but the spawning season. Both MEL and 5-MTL administrations arrested vitellogenesis during the gonadal recrudescence phase. As a result, stage I previtellogenic oocytes showed a significant increase in the MEL group in the preparatory and prespawning seasons, and in the 5-MTL group only in the prespawning season. Almost the same trend was noticed in the percentage distribution of stage 2 and 3 oocytes. The percentage distribution of stage 4 postvitellogenic oocytes decreased significantly during the preparatory and prespawning seasons, but not in the spawning season. The indole treatment resulted in a highly significant increase in the percentage distribution of atretic oocytes during prespawning, spawning, and post-spawning phases. Total protein content of the ovary was significantly lower in all seasons after MEL treatment, and only in the preparatory and prespawning seasons following 5-MTL treatment. The ovarian glycogen level was significantly lower in the preparatory and prespawning seasons (MEL group), and in the 5-MTL group only in the prespawning phase. Ascorbic acid level was inhibited by the indole treatment in all seasons except in the 5-MTL group during the post-spawning season. The results show that both MEL and 5-MTL are effective in inhibiting ovarian vitellogenesis and inducing atresia in the catfish.
Joy
K P
KP
Department of Zoology, Banaras Hindu University, Varanasi, India.
Agha
A K
AK
D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D002397:Catfishes; D004334:Drug Administration Schedule; D005260:Female; D005496:Follicular Atresia / Q000187:drug effects; D006003:Glycogen / Q000378:metabolism; D007211:Indoles / Q000008:administration & dosage / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D008550:Melatonin / Q000008:administration & dosage / Q000494:pharmacology*; D009865:Oocytes / Q000187:drug effects; D010053:Ovary / Q000187:drug effects*; D012621:Seasons; D014818:Vitellogenesis / Q000187:drug effects
D007211:Indoles; C002465:methoxytryptophol; D006003:Glycogen; D008550:Melatonin; D001205:Ascorbic Acid