title: Computer aided tracing and encoding of axonal arborizations.
abstract: This paper reports a method of acquiring and codifying branching profiles of axonal arborizations. Using a microcomputer interfaced with a motor driven microscope scanning stage, an acquisition strategy was developed. This strategy allows large profiles (covering up to 1250 mm2) to be measured with an accuracy of a few microns. Each histological section is associated with a specific coordinate system. This system was found to locate the profiles with high precision and reliability in the x-y microscope stage. The overall tracing accuracy of the system is fully discussed.
authors.affiliation: Istituto di Neurofisiologia CNR, Pisa, Italy.
authors.forename: G M
authors.initials: GM
authors.lastname: Ratto
authors.forename: C
authors.initials: C
authors.lastname: Usai
mesh_terms: D001369:Axons / Q000648:ultrastructure*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D012160:Retina / Q000166:cytology; D012984:Software