Importance of detection of SS-A/Ro autoantibody in screening immunofluorescence tests for autoantibodies to nuclear antigens.
SS-A/Ro autoantibodies are related to Sjögren's syndrome and to several clinical subsets of lupus erythematosus. The most widely used laboratory procedure for ANA screening is the indirect immunofluorescence test (IF-ANA); the laboratory detection of anti-SS-A/Ro requires implementation and adherence to several technical and quality assurance recommendations. Using appropriate substrate cells containing the SS-A/Ro antigen, many so-called "ANA-negative" lupus erythematosus patients will demonstrate a positive IF-ANA.
Bylund
D J
DJ
Department of Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037.
Nakamura
R M
RM
D000974:Antibodies, Antinuclear / Q000276:immunology*; D001324:Autoantigens / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007231:Infant, Newborn; D008178:Lupus Erythematosus, Cutaneous / Q000175:diagnosis* / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis* / Q000276:immunology; D011785:Quality Assurance, Health Care; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D012859:Sjogren's Syndrome / Q000175:diagnosis* / Q000276:immunology; D000099207:SS-A Antigen
D000974:Antibodies, Antinuclear; D001324:Autoantigens; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C116006:RO60 protein, human