A gene tightly linked to CEN6 is important for growth of Saccharomyces cerevisiae.
Transcriptional analysis of the region flanking the left boundary of the centromere of chromosome VI revealed the presence of a gene immediately adjacent to CEN6. The transcription of the gene is directed toward the centromere, and nucleotide sequence analysis showed that the coding region terminates only 50 bp away from CEN6. Our results extend to chromosome VI the observation that centromere-flanking regions of S. cerevisiae are transcriptionally active. Disruption of the coding region of the gene showed that its product, whilst not essential for cell viability, is important for normal cell growth. The gene has been termed DEG1 (DEpressed Growth rate). Comparison of the deduced amino acid sequence of DEG1 with a protein sequence databank revealed homology with the enzyme tRNA pseudouridine synthase I of E. coli.
Carbone
M L
ML
Dipartimento di Genetica e di Biologia dei Microrganismi, Università di Milano, Italy.
Solinas
M
M
Sora
S
S
Panzeri
L
L
D000595:Amino Acid Sequence; D001483:Base Sequence; D003062:Codon; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D008040:Genetic Linkage; D019751:Intramolecular Transferases; D007535:Isomerases / Q000235:genetics; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D003062:Codon; D004271:DNA, Fungal; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D007535:Isomerases; D019751:Intramolecular Transferases; C068903:DEG1 protein, S cerevisiae; C023199:tRNA-pseudouridine synthase I