Bombesin produces hypothermia in rats pretreated with 2-deoxy-D-glucose.
Previous research has shown that microinfusion of bombesin into the preoptic area (POA) decreases core body temperature in rats that are food-deprived or made hypoglycemic with insulin. The present study employed 2-deoxy-D-glucose, a competitive inhibitor of glycolysis, to further investigate the importance of a reduction in glucose utilization in the production of bombesin-induced hypothermia. Rats (n = 7) were pretreated with 2-DG (0, 25, 50, 100, 200 mg/kg; IP) followed by bombesin (100 ng/1.0 microliters) microinfusions into the POA. The highest dose of 2-DG (200 mg) was also tested in the absence of bombesin as a control. Pretreatment with 2-DG resulted in a dose-related reduction in Tb following bombesin. Injections of 2-DG alone did not significantly alter Tb. The results provide additional evidence that the production of bombesin-induced hypothermia in fasted rats is linked to a reduction in glucose utilization.
Babcock
A M
AM
Department of Psychology, University of South Alabama, Mobile 36688.
Maisel
M M
MM
D000818:Animals; D001831:Body Temperature / Q000187:drug effects*; D001839:Bombesin / Q000494:pharmacology*; D003847:Deoxyglucose / Q000494:pharmacology*; D005260:Female; D008845:Microinjections; D011301:Preoptic Area; D051381:Rats; D011919:Rats, Inbred Strains
D003847:Deoxyglucose; D001839:Bombesin