DNA fingerprinting of eukaryote genomes by synthetic oligodeoxyribonucleotide probes.
An extreme level of DNA sequence polymorphism, the basis of DNA fingerprinting, was first demonstrated using genome derived cloned probes. Subsequently, it was shown that DNA fingerprinting can also be carried out using short synthetic oligodeoxyribonucleotide probes specific for simple repetitive sequences. Further, in addition to radioactively labeled probes, non-radioactive oligonucleotides generate equally informative hybridization patterns. We discuss the development in the area of DNA fingerprinting and its future scope with respect to plant, animal and the human DNA.
Ali
S
S
National Institute of Immunology, Shahid Jeet Singh Marg, New Delhi, India.
Epplen
J T
JT
D000818:Animals; D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods*; D005057:Eukaryotic Cells; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D010944:Plants; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D015345:Oligonucleotide Probes