Immunohistochemical detection of NSE and S-100 protein in the thalamic VB nucleus after ablation of somatosensory cortex in the rat.
The ventrobasal (VB) nucleus has been studied after ablation of somatosensory cortex in 39 adult rats by the application of both NSE- and S-100 protein-immunoreactivity. Both NSE- and S-100 protein-immunoreactivity are confirmed in neurons and reactive astrocytes in the affected VB area and its surroundings, respectively. The NSE-immunoreactivity first starts in the affected VB at seven days postlesion and appears more active in its surrounding area at fourteen days postlesion. At the twenty-eight days, NSE-positive neurons are reduced in number and their stainability becomes weak. The time course of NSE-immunoreactivity is based on the progression of neuronal damage. And it is conceivable that the accumulation of NSE in neurons correlates with the regeneration. The S-100 protein-immunoreactivity is also first detected in the affected area at seven days postlesion and spread in its surrounding area at fourteen days postlesion. At twenty-eight days, S-100 protein-positive astrocytes are reduced in cell volume and their processes become thin. The time course of S-100 protein-immunoreactivity correlates with the degree of astrocytic hypertrophy. And the potent accumulation of S-100 protein appears after the onset of gliosis. The onset of neuronal damage and the repair process can be followed with immunohistochemical technique for both NSE and S-100 protein morphologically. Namely, NSE and S-100 protein can be of potential use as markers for destructive processes in the CNS.
Yamamoto
C
C
Department of Anatomy, Hamamatsu University School of Medicine, Japan.
Kawana
E
E
D000818:Animals; D015415:Biomarkers; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis; D008297:Male; D010751:Phosphopyruvate Hydratase / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D009418:S100 Proteins / Q000032:analysis*; D013003:Somatosensory Cortex / Q000601:surgery; D013787:Thalamic Nuclei / Q000737:chemistry*
D015415:Biomarkers; D007797:Laminin; C030564:S-100 calcium-binding protein alpha subunit; D009418:S100 Proteins; D010751:Phosphopyruvate Hydratase