Comparison of enzyme immunoassay and fluorescence polarization immunoassay as techniques for measuring anticonvulsant drugs on the same analytical instrument.
We have applied kits for enzyme immunoassay and fluorescence polarization immunoassay of anticonvulsant drugs to the same centrifugal analyser. There was a good correlation between the two techniques for the assay of phenytoin, carbamazepine, and phenobarbitone. Within- and between-batch reproducibility was also comparable but only the fluorescence polarization system allowed the use of stored calibration curves. The ability to use stored curves may be particularly advantageous to laboratories required to run a large number of stat assays or who are handling low workloads of particular analytes.
Thomas
K C
KC
Department of Chemical Pathology, East Glamorgan General Hospital, Pontypridd, Mid Glamorgan, Wales.
Hullin
D A
DA
Davis
S J
SJ
D002220:Carbamazepine / Q000097:blood*; D016231:Fluorescence Polarization Immunoassay / Q000379:methods; D006801:Humans; D007124:Immunoenzyme Techniques; D010634:Phenobarbital / Q000097:blood*; D010672:Phenytoin / Q000097:blood*
D002220:Carbamazepine; D010672:Phenytoin; D010634:Phenobarbital