title: The primary structure of the hemoglobins of a southern hemisphere lamprey (Mordacia mordax, Cyclostomata).
abstract: Mordacia mordax is a southern hemisphere lamprey belonging to Mordaciidae, a primitive family of Cyclostomata. Adult erythrocytes contain three monomeric hemoglobins which can be easily separated by cellulose acetate electrophoresis and isolated by ion-exchange chromatography. The N-terminal regions, and the tryptic peptides from each chain were submitted to automated Edman degradation; the alignment of the fragments was obtained by homology with the other Petromyzonoidea hemoglobins hitherto sequenced. Our results confirm the phylogenic distance between lampreys and hag-fish hemoglobins. As was observed for Petromyzon marinus species, two hemoglobins of Mordacia mordax are very close, as they differ only at 7 positions.
authors.affiliation: Département de Biochimie Médicale, Université de Bordeaux II.
authors.forename: I
authors.initials: I
authors.lastname: Hombrados
authors.forename: Y
authors.initials: Y
authors.lastname: Vidal
authors.forename: K
authors.initials: K
authors.lastname: Rodewald
authors.forename: G
authors.initials: G
authors.lastname: Braunitzer
authors.forename: E
authors.initials: E
authors.lastname: Neuzil
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002852:Chromatography, Ion Exchange; D004588:Electrophoresis, Cellulose Acetate; D004912:Erythrocytes / Q000737:chemistry; D005914:Globins / Q000032:analysis; D006454:Hemoglobins / Q000032:analysis*; D006868:Hydrolysis; D007798:Lampreys / Q000097:blood*; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification; D014357:Trypsin
chemical_list: D000596:Amino Acids; D006454:Hemoglobins; D010455:Peptides; D005914:Globins; D014357:Trypsin