title: Influence of airborne suspended matter on mitotic cell division.
abstract: The effect of organic extracts of airborne suspended matter collected in the highly polluted industrial region of Silesia (Poland) on mitotic cell division was evaluated in the Chinese hamster V79 cell line. Crude benzene extracts as well as sequential elution solvent chromatography (SESC) fractions were investigated for their ability to affect the mitotic index, the proportion of anaphases-telophases to metaphases (AT/M ratio), the cloning efficiency and to produce aneuploid cells. The incidence of cell division disturbances in V79 cells exposed to extracts increased in a concentration-dependent manner. Mitotic arrest, manifested as a highly increased mitotic index and a concomitant decrease in the AT/M ratio, was found for the crude extract at a dose corresponding to 0.75 m3 of air. Comparable effects were noticed for SESC fraction 4, probably containing monophenol compounds. A strong dose-dependent reduction of cloning efficiency of V79 cells demonstrated cytotoxic activity of both the crude extract and fraction 4.
authors.affiliation: Department of Tumor Biology, Institute of Oncology, Gliwice, Poland.
authors.forename: G
authors.initials: G
authors.lastname: Motykiewicz
authors.forename: W
authors.initials: W
authors.lastname: Hadnagy
authors.forename: N H
authors.initials: NH
authors.lastname: Seemayer
authors.forename: J
authors.initials: J
authors.lastname: Szeliga
authors.forename: A
authors.initials: A
authors.lastname: Tkocz
authors.forename: M
authors.initials: M
authors.lastname: Chorazy
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D000782:Aneuploidy; D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D002850:Chromatography, Gel; D002869:Chromosome Aberrations; D002999:Clone Cells / Q000502:physiology; D006224:Cricetinae; D008938:Mitosis; D008940:Mitotic Index; D011044:Poland; D012905:Smog / Q000009:adverse effects; D008941:Spindle Apparatus
chemical_list: D000393:Air Pollutants; D012905:Smog