title: Odontoblast-like cytodifferentiation of human dental pulp cells in vitro in the presence of a calcium hydroxide-containing cement.
abstract: The cement produced microcrystals of calcite by reaction with culture medium supplemented with calf serum. Human dental pulp cells seeded on such a substrate preferentially adhered and aggregated around the microcrystals. Immunofluorescence and immunogold labelling revealed a high affinity of serum fibronectin molecules for the calcite crystals. At 4 weeks in culture, the cells had various features of differentiated odontoblasts, notably nuclear polarization, typical appearance of the Golgi apparatus, synthesis of type I collagen and absence of type III, and apical accumulation of actin and vimentin. These cells also elaborated a collagenous extracellular matrix which did not mineralize.
authors.affiliation: Laboratoire d'Histophysiologie et de Pathologie des Tissus Dentaires, UA CNRS 244, Faculté d'Odontologie, Lyon, France.
authors.forename: D
authors.initials: D
authors.lastname: Seux
authors.forename: M L
authors.initials: ML
authors.lastname: Couble
authors.forename: D J
authors.initials: DJ
authors.lastname: Hartmann
authors.forename: J P
authors.initials: JP
authors.lastname: Gauthier
authors.forename: H
authors.initials: H
authors.lastname: Magloire
mesh_terms: D000293:Adolescent; D002126:Calcium Hydroxide / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002648:Child; D003094:Collagen / Q000032:analysis; D003461:Crystallography; D003738:Dental Cements / Q000494:pharmacology*; D003782:Dental Pulp / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects; D004577:Electron Probe Microanalysis; D005353:Fibronectins / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008903:Minerals / Q000494:pharmacology; D009804:Odontoblasts / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects; D014404:Tubulin / Q000032:analysis; D014746:Vimentin / Q000032:analysis; D014961:X-Ray Diffraction
chemical_list: D003738:Dental Cements; D005353:Fibronectins; D008903:Minerals; D014404:Tubulin; D014746:Vimentin; C025702:Dycal; D003094:Collagen; D002126:Calcium Hydroxide