Successful co-culture of 1-4-cell cattle ova to the morula or blastocyst stage.
Bovine ova (n = 326) collected at the 1-4-cell stage were cultured in TCM-199 + 10% foetal calf serum with or without oviducal cells. The bovine oviducal cells were collected and seeded either on the day of ovum recovery (BOC-0) or 3 days earlier (BOC-3). In Exp. 1, the effect of age of oviducal cells in co-culture on ovum development was examined. In the BOC-0 and BOC-3 treatments, respectively, 36/46 (78%) and 30/37 (81%) of ova developed to morulae or blastocysts, while no ova developed past the 8-16-cell stage in the absence of oviducal cells. In Exp. 2, the effect of age of oviducal cells and of physical contact between the oviducal cells and ova on ovum development was examined. In the BOC-0 and BOC-3 treatments, respectively, 29/42 (69%) and 23/43 (53%) of the ova developed to morulae or blastocysts, while 1/42 (2%) developed to the morula stage in the absence of oviducal cells. Physical separation of the ova using a microporous membrane inserted between the oviducal cells and the ova did not affect ovum development, with 26/42 (62%) and 22/42 (52%) of ova developing to morulae or blastocysts in the BOC-0 and BOC-3 treatments, respectively. A high proportion of the morulae and blastocysts in Exp. 1 (57/66, 86%) and Exp. 2 (67/100, 67%) were of quality grades 1 or 2, with mean nuclei counts of 85 for morulae and 111 for blastocysts.(ABSTRACT TRUNCATED AT 250 WORDS)
McCaffrey
C
C
Agriculture and Food Development Authority, Belclare, Tuam, Co. Galway, Ireland.
McEvoy
T G
TG
Diskin
M G
MG
Gwazdauskas
F C
FC
Kane
M T
MT
Sreenan
J M
JM
D000818:Animals; D001755:Blastocyst / Q000502:physiology; D002417:Cattle; D002450:Cell Communication / Q000502:physiology; D002452:Cell Count; D002469:Cell Separation; D002478:Cells, Cultured; D005260:Female; D009028:Morula / Q000502:physiology; D010063:Ovum / Q000254:growth & development*; D013997:Time Factors