13C-NMR: a simple yet comprehensive method for analysis of intermediary metabolism.
13C-NMR is a particularly attractive tool for metabolic studies because of its inherent simplicity: all labeled products at sufficient concentration may be identified and analysed in a single spectrum. However, the real power behind the approach presented here is the ability to measure groups of individual 13C-isotopomers (isotope isomers). The information that this provides is superior to conventional tracer techniques, allowing a very detailed description of metabolic events. Several examples are given of the value of this convenient and straightforward analysis for some problems of current interest in intermediary metabolism.
Jeffrey
F M
FM
Mary Nell and Ralph B. Rogers Magnetic Resonance Center, Department of Radiology, Dallas 75235-8573.
Rajagopal
A
A
Malloy
C R
CR
Sherry
A D
AD
D000818:Animals; D002247:Carbon Isotopes; D002952:Citric Acid Cycle; D003198:Computer Simulation; D005227:Fatty Acids / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009206:Myocardium / Q000378:metabolism; D051381:Rats
D002247:Carbon Isotopes; D005227:Fatty Acids