Interleukins 1, 4 and 6 induce the colony formation of human bone marrow B lineage cells.
An agar-based, B cell colony assay (McGinnes, K. et al., Blood 1990. 76:896) has been used to study the influence of known cytokines on the growth of B lineage colonies initiated by cells from normal, human bone marrow samples. We demonstrate that a combination of interleukin (IL) 1, IL4 and IL6 act directly to promote the generation of plaque-forming colonies. IL6 was shown to act at a late stage of colony formation, which is consistent with its role in the induction of immunoglobulin secretion from mature B cells. In contrast, IL 1 and IL 4 were required at earlier stages in the formation of colonies containing cells which secrete immunoglobulin.
McGinnes
K
K
Division of Cell and Molecular Biology, Ontario Cancer Institute, Toronto, Canada.
Paige
C J
CJ
D001402:B-Lymphocytes / Q000187:drug effects* / Q000502:physiology; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D006410:Hematopoiesis / Q000187:drug effects*; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000494:pharmacology*; D015851:Interleukin-7 / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects
D007375:Interleukin-1; D015850:Interleukin-6; D015851:Interleukin-7; D015847:Interleukin-4