Culture of human outer root sheath cells from plucked hair follicles in serum-free conditions.
We succeeded in culturing human outer root sheath cells (ORSC) in serum-free conditions without a biological feeder layer. The combination of collagen type IV substrate and modified MCDB 153 medium supplemented with bovine pituitary gland extract (K-GM medium) could support the growth of ORSC. These cells can be serially cultivated for at least 4 passages and stored in liquid nitrogen with good recovery. Thus, a large series of experiments using ORSC may be run simultaneously.
Arase
S
S
Department of Dermatology, School of Medicine, University of Tokushima, Japan.
Katoh
S
S
Sadamoto
Y
Y
Nakanishi
H
H
Urano
Y
Y
Fujie
K
K
Takeda
K
K
D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen; D003114:Colony-Forming Units Assay; D003470:Culture Media; D006197:Hair / Q000166:cytology*; D006801:Humans
D003470:Culture Media; D003094:Collagen