Studies on the intracellular distributions of soluble epoxide hydrolase and of catalase by digitonin-permeabilization of hepatocytes isolated from control and clofibrate-treated mice.
Digitonin permeabilization of hepatocytes from control and clofibrate-treated (0.5% by mass, 10 days) male C57bl/6 mice was used to study the intracellular distributions of soluble ('cytosolic') epoxide hydrolase and of catalase. The following conclusions were drawn. (1) About 60% of the total soluble epoxide hydrolase activity in control mouse hepatocytes is situated in the cytosol. (2) The rest is not mitochondrial, but probably peroxisomal. (3) Of the total catalase activity in control mouse hepatocytes, 5-10% is found in the cytosol. (4) Treatment of mice with clofibrate increases the total hepatocyte activity of soluble epoxide hydrolase 4-fold, but does not influence the relative distribution of this enzyme between cytosol and peroxisomes. (5) The total catalase activity is increased 3.5-fold by clofibrate treatment and 15-35% of this activity is shifted from the peroxisomes to the cytosol.
Eriksson
A M
AM
Department of Biochemistry, University of Stockholm, Sweden.
Zetterqvist
M A
MA
Lundgren
B
B
Andersson
K
K
Beije
B
B
DePierre
J W
JW
D000818:Animals; D002374:Catalase / Q000032:analysis* / Q000378:metabolism*; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D002994:Clofibrate / Q000494:pharmacology*; D004072:Digitonin / Q000494:pharmacology*; D004851:Epoxide Hydrolases / Q000032:analysis / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012016:Reference Values; D013347:Subcellular Fractions / Q000201:enzymology
D002374:Catalase; D004851:Epoxide Hydrolases; D002994:Clofibrate; D004072:Digitonin