title: Evaluation of a hygiene monitor for detection of contamination in dental surgeries.
abstract: Routines for disinfecting working surfaces in dental surgeries are difficult to monitor without time-consuming and labour-intensive microbiological techniques, yet effective monitoring is a vital part of cross-infection control. Easy to use, on-site methods would be valuable in this context. This study evaluates a portable monitor, the Biotrace Hygiene Monitor, which uses bioluminescence to measure adenosine triphosphate (ATP) on surfaces. Under laboratory conditions, the ability of the monitor to detect whole saliva and Streptococcus sanguis was determined and, in the general practice environment, the level of ATP on surfaces in five dental surgeries was assessed. The minimum amount of saliva detectable was 0.5 microliters and in surgeries, the monitor readily identified numerous surfaces with fairly high levels of ATP. Routine cleaning methods sometimes left ATP on surfaces at levels which represented a cross-infection risk, if it is assumed that the ATP derived from patients' saliva. Modification of cleaning methods resulted in a reduction of ATP levels to within that which could be considered reasonably practicably safe. It is concluded that the Biotrace Hygiene Monitor offers a simple and valuable means of monitoring dental practice cleaning routines.
authors.affiliation: Department of Oral Pathology, School of Clinical Denstistry, University of Sheffield.
authors.forename: C W
authors.initials: CW
authors.lastname: Douglas
authors.forename: P S
authors.initials: PS
authors.lastname: Rothwell
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D003140:Communicable Disease Control / Q000295:instrumentation* / Q000379:methods; D003770:Dental Offices; D004784:Environmental Monitoring / Q000295:instrumentation*; D005069:Evaluation Studies as Topic; D006801:Humans; D008164:Luminescent Proteins; D012463:Saliva; D013298:Streptococcus sanguis / Q000032:analysis
chemical_list: D008164:Luminescent Proteins; D000255:Adenosine Triphosphate