Identification of sn-2-omega-hydroxycarboxylate-containing phospholipids in a lipid extract from bovine brain.
Phospholipids having both a long-chain acyl (palmitoyl or stearoyl) and a short-chain hydroxycarboxylyl (C3-C9) residue were identified by GC-MS in a fraction with PAF-like activity from a bovine brain lipid extract. The hydroxyl group in the hydroxycarboxylate residue was determined to be at the omega-position by comparison of the mass spectra of the tert-butyl-dimethylsilyl derivatives of these compounds with those of synthetic hydroxybutyrate-containing phosphatidylcholines. The co-existence of short-chain hydroxycarboxylate-, monocarboxylate- and dicarboxylate-containing phospholipids in the bovine brain lipid extract suggested that these compounds were formed by peroxidation of membrane phospholipids, especially phosphatidylcholines.
Tokumura
A
A
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Tanaka
T
T
Yotsumoto
T
T
Tsukatani
H
H
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D008401:Gas Chromatography-Mass Spectrometry; D006880:Hydroxy Acids / Q000032:analysis*; D015394:Molecular Structure; D010743:Phospholipids / Q000737:chemistry* / Q000302:isolation & purification; D010972:Platelet Activating Factor / Q000302:isolation & purification
D006880:Hydroxy Acids; D010743:Phospholipids; D010972:Platelet Activating Factor