Altered phospholipase C activity in renal medulla of DOCA-salt hypertensive rats.
We investigated the alterations of phospholipase C activity in the kidney and arterial wall of deoxycorticosterone acetate (DOCA)-salt hypertensive rats. Neither DOCA nor salt administration alone affected phospholipase C activity in the kidney and arterial wall. In contrast, when the rats were given both DOCA and salt and developed high blood pressure, the phospholipase C activity became greater in the outer and inner medulla (papilla) while the enzyme activity was reduced significantly in the renal cortex and arterial wall. Such an increase in phospholipase C activity was not observed in the kidney of a genetic rat for spontaneous hypertension. Moreover, the phospholipase A2 activity was also stimulated in the renal medulla of DOCA-salt hypertensive rats. Thus, these data indicate that DOCA-salt hypertension is associated with an increase in phospholipase C activity in the renal medulla, which seems primary to the development of DOCA-salt hypertension.
Uehara
Y
Y
2nd Department of Medicine, University of Tokyo, Japan.
Numabe
A
A
Takada
S
S
Hirawa
N
N
Matsuoka
H
H
Ikeda
T
T
Yagi
S
S
Sugimoto
T
T
D000818:Animals; D003900:Desoxycorticosterone / Q000009:adverse effects; D006973:Hypertension / Q000139:chemically induced / Q000201:enzymology*; D007679:Kidney Medulla / Q000201:enzymology*; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D051381:Rats; D011918:Rats, Inbred SHR; D011919:Rats, Inbred Strains; D011921:Rats, Inbred WKY; D012965:Sodium Chloride / Q000009:adverse effects; D010738:Type C Phospholipases / Q000378:metabolism*
D003900:Desoxycorticosterone; D012965:Sodium Chloride; D010741:Phospholipases A; D054467:Phospholipases A2; D010738:Type C Phospholipases