title: Incorporation of exogenous uracil by Cryptosporidium parvum in vitro.
abstract: Oocysts of Cryptosporidium parvum were used to infect Madin-Darby bovine kidney cells. Cultures were incubated in a reduced-oxygen atmosphere in candle jars or in a 5% CO2-95% air atmosphere. At 72 h, parasites were quantitated microscopically and found to be enhanced 5.5-fold in the reduced-oxygen atmosphere. Using candle jars, we then determined that C. parvum was amenable to [3H]uracil incorporation assays and easily quantitated with this method.
authors.affiliation: Division of Biology, Kansas State University, Manhattan 66506.
authors.forename: S J
authors.initials: SJ
authors.lastname: Upton
authors.forename: M
authors.initials: M
authors.lastname: Tilley
authors.forename: R R
authors.initials: RR
authors.lastname: Mitschler
authors.forename: B S
authors.initials: BS
authors.lastname: Oppert
mesh_terms: D000818:Animals; D002460:Cell Line; D003458:Cryptosporidium / Q000254:growth & development / Q000378:metabolism*; D014498:Uracil / Q000378:metabolism*
chemical_list: D014498:Uracil