title: Source and role of diacylglycerol formed during phagocytosis of opsonized yeast particles and associated respiratory burst in human neutrophils.
abstract: The results presented in this paper demonstrate that in human neutrophils phagocytosis of C3b/bi and IgG-opsonized yeast particles is associated with activation of phospholipase D and that this reaction is the main source of diglycerides. The demonstration is based upon the following findings: 1) the challenge of neutrophils with these opsonized particles was followed by a rapid formation of [3H]alkyl-phosphatidic acid [( 3H]alkyl-PA) and [3H]alkyl-diglyceride [( 3H]alkyl-DG) in cells labeled with [3H]alkyl-lyso-phosphatidylcholine; 2) in the presence of ethanol [3H]alkyl-phosphatidylethanol was formed, and accumulation of [3H]alkyl-PA and [3H]alkyl-DG was depressed; 3) propranolol, by inhibiting the dephosphorylation of [3H]alkyl-PA, completely inhibited the accumulation of [3H]alkyl-DG and depressed by about 75% the formation of diglyceride mass. Evidence is also presented that phagocytosis of C3b/bi and IgG-opsonized yeast particles and associated respiratory burst can take place independently of diglyceride formation and of the activity of this second messenger on protein kinase C. In fact: a) propranolol while completely inhibited the formation of diglyceride mass did not modify either the phagocytosis or respiratory burst; b) these two processes were insensitive to staurosporine.
authors.affiliation: Institute of General Pathology, University of Verona, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Della Bianca
authors.forename: M
authors.initials: M
authors.lastname: Grzeskowiak
authors.forename: D
authors.initials: D
authors.lastname: Lissandrini
authors.forename: F
authors.initials: F
authors.lastname: Rossi
mesh_terms: D000470:Alkaloids / Q000494:pharmacology; D003179:Complement C3b / Q000502:physiology; D004075:Diglycerides / Q000097:blood*; D006801:Humans; D007074:Immunoglobulin G; D066298:In Vitro Techniques; D007700:Kinetics; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D010101:Oxygen Consumption / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects; D010739:Phospholipase D / Q000097:blood; D010743:Phospholipids / Q000097:blood*; D011433:Propranolol / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D012441:Saccharomyces cerevisiae; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014316:Tritium
chemical_list: D000470:Alkaloids; D004075:Diglycerides; D007074:Immunoglobulin G; D010743:Phospholipids; D014316:Tritium; D009240:N-Formylmethionine Leucyl-Phenylalanine; D003179:Complement C3b; D011433:Propranolol; D011493:Protein Kinase C; D010739:Phospholipase D; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate