title: Cloning of human alpha 1(X) collagen DNA and localization of the COL10A1 gene to the q21-q22 region of human chromosome 6.
abstract: With consensus primers based upon the nucleotide sequence of the chicken alpha 1(X) collagen gene, we have used PCR with human genomic DNA as template to isolate a 289 bp fragment coding for part of the carboxyl non-triple helical domain of the human alpha 1(X) gene. We have demonstrated the presence of the sequence of the PCR clone within the human genome by partial sequence analysis of a 1 kb HindIII genomic DNA fragment that hybridized with the PCR clone. Furthermore, using the PCR clone as a probe for in situ hybridization of human metaphase chromosome spreads, and for Southern analysis of a panel of human-hamster somatic cell hybrid DNAs, we have assigned the locus for the alpha 1(X) gene to the q21-q22 region of human chromosome 6.
authors.affiliation: Department of Anatomy and Cellular Biology, Harvard Medical School, Boston, MA 02115.
authors.forename: S
authors.initials: S
authors.lastname: Apte
authors.forename: M G
authors.initials: MG
authors.lastname: Mattei
authors.forename: B R
authors.initials: BR
authors.lastname: Olsen
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002896:Chromosomes, Human, Pair 6; D003001:Cloning, Molecular; D003094:Collagen / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: D003094:Collagen