title: [Histochemical investigations of the intracellular localization of adenylate cyclase activity after mitogenic or beta-adrenergic stimulation of T-lymphocytes of the mouse].
abstract: The role of AC at the beginning of an immune response is not fully understood until now. Using the specific substrate AMP-PNP we could show AC-activity in ConA-stimulated murine spleen lymphocytes histochemically. In contrast to beta-adrenergic stimulation (by Isoproterenol) ConA-induced AC-activity was found not only at the cell membrane and the nuclear membrane but also at some structures of the Golgi apparatus of the cells. The difference in AC-activity after beta-adrenergic or mitogenic stimulation is discussed.
authors.affiliation: Institut für Biochemie, Universität Rostock, Bundesrepublik Deutschland.
authors.forename: D
authors.initials: D
authors.lastname: Paukstat
authors.forename: W
authors.initials: W
authors.lastname: Schulze
authors.forename: A
authors.initials: A
authors.lastname: Henkel
authors.forename: C
authors.initials: C
authors.lastname: Hückel
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002451:Cell Compartmentation; D002462:Cell Membrane / Q000201:enzymology; D003208:Concanavalin A / Q000494:pharmacology; D006056:Golgi Apparatus / Q000201:enzymology; D006651:Histocytochemistry; D007545:Isoproterenol / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008808:Mice, Inbred CBA; D009685:Nuclear Envelope / Q000201:enzymology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
chemical_list: D003208:Concanavalin A; D000262:Adenylyl Cyclases; D007545:Isoproterenol
vernacular_title: Histochemische Untersuchungen zur intrazellulären Lokalisation von Adenylatzyklase-Aktivität nach mitogener bzw. beta-adrenerger Stimulierung von T-Lymphozyten der Maus.