title: Purification of lipopolysaccharide from strains of Yersinia enterocolitica belonging to serogroups 03 and 09.
abstract: Lipopolysaccharide (LPS) was purified from strains of Yersinia enterocolitica belonging to serogroups 03 and 09, by three methods, and analysed by SDS-PAGE and silver staining for carbohydrate. SDS-PAGE of LPS prepared from whole-cells by digestion with proteinase-K, produced profiles containing high molecular mass LPS and a lower molecular mass region migrating as discrete bands. LPS prepared from strains belonging to serogroup 03, using a hot-phenol procedure alone was found to contain cellular proteins, and LPS prepared from strains of serogroup 09, by this method, did not contain high molecular mass carbohydrate. A novel method of preparing LPS by digesting bacterial outer membranes with proteinase-K prior to hot-phenol extraction produced protein-free LPS from strain of Y. enterocolitica 03 and high molecular mass LPS from strains belonging to serogroup 09.
authors.affiliation: Division of Enteric Pathogens, Central Public Health Laboratory, London, U.K.
authors.forename: H
authors.initials: H
authors.lastname: Chart
authors.forename: B
authors.initials: B
authors.lastname: Rowe
mesh_terms: D002462:Cell Membrane / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D008070:Lipopolysaccharides / Q000302:isolation & purification*; D008970:Molecular Weight; D013045:Species Specificity; D015008:Yersinia enterocolitica / Q000145:classification / Q000276:immunology*
chemical_list: D008070:Lipopolysaccharides