Production and characterization of antibodies against nivalenol tetraacetate.
Antibody against nivalenol tetraacetate (tetra-Ac-NIV) was prepared by immunization of rabbits with triacetyl-15-pimelate-NIV conjugated to bovine serum albumin. By using tritiated tetra-Ac-NIV as the test ligand, antibody titers were demonstrated as early as 4 weeks after immunization. Useful antibody for radioimmunoassay (RIA) of tetra-Ac-NIV was obtained 7 weeks after immunization, with one booster injection. Results of competitive RIA revealed that the antibody was most specific to tetra-Ac-NIV. The relative cross-reactivity of this antibody with tetra-Ac-NIV, deoxynivalenol triacetate, and neosolaniol triacetate was found to be 100, 2.2, and less than 1, respectively. Practically no cross-reaction was found with deoxynivalenol, fusarenon X, and NIV. The detection limit for tetra-Ac-NIV by RIA was about 5.0 ng/ml (0.5 ng per assay). The use of this antibody for quantitation of NIV in cereals after acetylation of sample extracts is proposed.
Wang
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Food Research Institute, University of Wisconsin-Madison 53706.
Chu
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FS
D000107:Acetylation; D000818:Animals; D000908:Antibodies, Fungal / Q000096:biosynthesis*; D000918:Antibody Specificity; D005670:Fusarium / Q000276:immunology; D015394:Molecular Structure; D009183:Mycotoxins / Q000276:immunology*; D011817:Rabbits; D011863:Radioimmunoassay; D014255:Trichothecenes / Q000276:immunology*
D000908:Antibodies, Fungal; D009183:Mycotoxins; D014255:Trichothecenes; C069217:nivalenol tetraacetate; C038405:nivalenol; C007262:deoxynivalenol