Site-specific recombination between homologous chromosomes in Drosophila.
The ability to mark a cell and its descendants genetically so that the resulting cell clone can be distinguished from neighboring cells facilitates studies in animal biology and development. A method of generating clones by inducing homologous mitotic recombination in Drosophila with a site-specific yeast recombinase is described. This method allows for frequent mosaicism after mitotic exchange is induced at predefined sites in the genome.
Golic
K G
KG
Department of Molecular Genetics and Cell Biology, Howard Hughes Medical Institute, University of Chicago, IL 60637.
D000818:Animals; D002874:Chromosome Mapping; D002875:Chromosomes / Q000502:physiology*; D003433:Crosses, Genetic; D004330:Drosophila / Q000235:genetics*; D005260:Female; D005838:Genotype; D008297:Male; D008938:Mitosis; D008957:Models, Genetic; D009030:Mosaicism; D011995:Recombination, Genetic