title: Detection of Escherichia coli alpha-haemolysin genes and their expression in a human faecal strain of Enterobacter cloacae.
abstract: Faecal samples of 200 infants were investigated for haemolytic Enterobacteriaceae. Forty infants were carrying alpha-haemolysin producing Escherichia coli, two carried haemolytic strains of Morganella morganii and one infant carried a haemolytic strain of Enterobacter cloacae. The M. morganii and E. cloacae strains were found to produce alpha-haemolysin which was tested with a specific monoclonal antibody and by DNA-hybridization with an alpha-haemolysin specific gene probe. To our knowledge this is the first report of alpha-haemolysin production found in a strain of E. cloacae.
authors.affiliation: Robert Koch-Institut des Bundesgesundheitsamtes, Berlin, F.R.G.
authors.forename: J
authors.initials: J
authors.lastname: Prada
authors.forename: L
authors.initials: L
authors.lastname: Beutin
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D004754:Enterobacter / Q000235:genetics*; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D015870:Gene Expression; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000235:genetics*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D010641:Phenotype
chemical_list: D006460:Hemolysin Proteins