title: Amino acid sequence analysis of a mouse interleukin 5 receptor protein reveals homology with a mouse interleukin 3 receptor protein.
abstract: A polypeptide chain for the mouse interleukin 5 receptor (IL5R) was purified from detergent-lysed B13 cells, a mouse IL5-dependent pre-B cell line. Purification was by a single immunoaffinity chromatographic step using an anti-mouse IL5R monoclonal antibody, R52. Internal amino acid sequence was obtained from four trypsin-generated peptides. All peptides were found to be present in the published amino acid sequence of a mouse IL3R and the mouse IL3R-like protein deduced from the cDNA. This indicates that the mouse IL5R and the mouse IL3R have a homologous polypeptide in common and suggests that the specificity of these lymphokine receptors is mainly generated by association with another ligand-specific polypeptide chain.
authors.affiliation: Roche Research Gent, Belgium.
authors.forename: R
authors.initials: R
authors.lastname: Devos
authors.forename: J
authors.initials: J
authors.lastname: Vandekerckhove
authors.forename: A
authors.initials: A
authors.lastname: Rolink
authors.forename: G
authors.initials: G
authors.lastname: Plaetinck
authors.forename: J
authors.initials: J
authors.lastname: Van der Heyden
authors.forename: W
authors.initials: W
authors.lastname: Fiers
authors.forename: J
authors.initials: J
authors.lastname: Tavernier
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D007377:Interleukin-3 / Q000502:physiology; D015848:Interleukin-5 / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D011971:Receptors, Immunologic / Q000032:analysis* / Q000302:isolation & purification; D018123:Receptors, Interleukin; D016185:Receptors, Interleukin-3 / Q000032:analysis*; D053649:Receptors, Interleukin-5
chemical_list: D000911:Antibodies, Monoclonal; D007377:Interleukin-3; D015848:Interleukin-5; D011971:Receptors, Immunologic; D018123:Receptors, Interleukin; D016185:Receptors, Interleukin-3; D053649:Receptors, Interleukin-5