title: Protein growth factor(s) from C6 glioma cells that promote the growth of murine hybridomas.
abstract: C6 glioma cell conditioned medium (C6CM) has been used as a growth supplement for murine hybridomas. At 10% C6CM has been found to increase cell proliferation by 3-4-fold. This effect is observed in the presence of saturating concentration of FCS. Clonal growth is also enhanced 6.7-fold. No growth promoting effect is seen on murine myelomas or spleen cells. The factor(s) appear to be protein in nature.
authors.affiliation: Department of Biochemistry, All India Institute of Medical Sciences, New Delhi.
authors.forename: K G
authors.initials: KG
authors.lastname: Gomathi
authors.forename: E
authors.initials: E
authors.lastname: Sharma
authors.forename: S K
authors.initials: SK
authors.lastname: Sharma
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D005910:Glioma / Q000503:physiopathology*; D006133:Growth Substances / Q000494:pharmacology*; D006825:Hybridomas / Q000166:cytology / Q000187:drug effects*; D015850:Interleukin-6 / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D011506:Proteins / Q000494:pharmacology*; D051381:Rats
chemical_list: D003470:Culture Media; D006133:Growth Substances; D015850:Interleukin-6; D011506:Proteins