Antagonistic effect of the antiestrogen 4-hydroxytamoxifen on estradiol-stimulated acetylation of nuclear high mobility group (HMG) proteins in the uterus of newborn guinea-pigs.
The effect of the antiestrogen 4-hydroxytamoxifen on the estradiol-stimulated acetylation of nuclear high mobility group (HMG) proteins was studied in the uterus of newborn (3-day-old) guinea-pig. 4-Hydroxytamoxifen (10(-6) M) selectively inhibits the stimulatory effect of estradiol (5 x 10(-8) M) on the acetylation of HMG-14 proteins 30 min after incubation with uterine tissue slices. No effect of 4-hydroxytamoxifen was observed on HMG-1 + HMG-2 or HMG-17 proteins. The data suggest that the blockage of HMG-14 acetylation is an early event in gene expression which is in relation to the antagonistic effect of the antiestrogen.
Bertrand-Mercat
P
P
C.N.R.S. Steroid Hormone Research Unit, Foundation for Hormone Research, Paris, France.
Pasqualini
J R
JR
D000107:Acetylation; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002467:Cell Nucleus / Q000378:metabolism; D004852:Epoxy Compounds / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology*; D004965:Estrogen Antagonists / Q000494:pharmacology*; D005260:Female; D006168:Guinea Pigs; D006609:High Mobility Group Proteins / Q000378:metabolism*; D009578:Nitrobenzenes / Q000494:pharmacology; D010664:Phenylmethylsulfonyl Fluoride / Q000378:metabolism; D011480:Protease Inhibitors / Q000494:pharmacology; D013629:Tamoxifen / Q000031:analogs & derivatives* / Q000494:pharmacology; D014361:Trypsin Inhibitors / Q000378:metabolism; D014599:Uterus / Q000378:metabolism*
D002087:Butyrates; D004852:Epoxy Compounds; D004965:Estrogen Antagonists; D006609:High Mobility Group Proteins; D009578:Nitrobenzenes; D011480:Protease Inhibitors; D014361:Trypsin Inhibitors; D013629:Tamoxifen; D020148:Butyric Acid; C058462:1,2-epoxy-1-(4-nitrophenyl)pentane; C016601:afimoxifene; D004958:Estradiol; D010664:Phenylmethylsulfonyl Fluoride