Establishment of a novel cell line with T-lineage phenotype (HPB-MLp-W) from a non-Hodgkin's lymphoma patient.
We report the characterization of a novel human T-cell line, HPB-MLp-W, which was established from blastic cells of a lymph node specimen from a patient with non-Hodgkin's lymphoma. They demonstrated the T-cell association antigens, CD2 and CD4, but no CD3, CD8, CD1, CD5, CD7 nor T-cell antigen receptor on their cell surfaces. They were also positive for Ia and Ki-1 antigen, and negative for CD25 (Tac-1). The cell line HPB-MLp-W had the same pattern of antigen expression as the patient's cells. Southern-blot analysis of DNA showed a rearrangement of the T-cell receptor-alpha and beta genes. To our knowledge, this is a novel cell line with unique T-lineage marker, to be established from a case of non-Hodgkin's lymphoma.
Morikawa
S
S
Department of Pathology, Shimane Medical University, Izumo, Japan.
Morikawa
K
K
Hara
J
J
Nagasaki
M
M
Nakano
A
A
Oseko
F
F
D015703:Antigens, CD / Q000032:analysis*; D001706:Biopsy; D002460:Cell Line; D002871:Chromosome Banding; D046508:Culture Techniques / Q000379:methods; D005260:Female; D015329:Gene Rearrangement, T-Lymphocyte; D006801:Humans; D007621:Karyotyping; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D008875:Middle Aged; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology*
D015703:Antigens, CD