Muscarinic receptors in separate populations of noradrenaline- and adrenaline-containing chromaffin cells.
We have performed binding experiments of (a)[3H]quinuclidinyl benzilate to partially purified membranes from noradrenaline- and adrenaline-containing chromaffin cells and (b) [3H]N-methyl-quinuclidinyl benzilate to acutely isolated, or 48-h cultured, chromaffin cells subpopulations. Using this approach, we obtained enough evidence to conclude (1st) that muscarinic receptors are present in both noradrenaline- and adrenaline containing cells; (2nd) that noradrenaline cells contain in fact 2-3 fold higher density of those receptors; and (3rd) that those receptors undergo plastic changes upon chronic culturing of the cells.
Michelena
P
P
Departamento de Farmacología, Facultad de Medicina, Universidad Autónoma de Madrid, Spain.
Moro
M A
MA
Castillo
C J
CJ
García
A G
AG
D000313:Adrenal Medulla / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D004837:Epinephrine / Q000032:analysis*; D007700:Kinetics; D009638:Norepinephrine / Q000032:analysis*; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D011869:Radioligand Assay; D011976:Receptors, Muscarinic / Q000378:metabolism*; D014316:Tritium
D011976:Receptors, Muscarinic; D014316:Tritium; D011813:Quinuclidinyl Benzilate; D009638:Norepinephrine; D004837:Epinephrine