Characterization of a 54-nucleotide gap region in the 28S rRNA gene of Schistosoma mansoni.
We have analyzed 572 bp in the 28S rDNA of the human blood fluke Schistosoma mansoni which correspond to expansion segment 5 of domain IV as defined by Clark et al. for the Xenopus laevis 28S rRNA. S1 nuclease mapping and primer extension analysis comparing this region with the mature 28S rRNA indicate that there are 54 nucleotides present in the 28S rDNA which are absent from the mature rRNA. This defines a gap that creates two 28S rRNA subunits (28S alpha and 28S beta). Comparison of the S. mansoni sequence with rDNAs of other organisms which contain gaps in their 28S rRNA shows that the overall features are conserved except that the S. mansoni gap is less A + T-rich. The conserved features include: (1) the location of the gap within the 28S rRNA; (2) the predicted secondary structure of the gap, containing a stem-loop with a UAAU sequence within the loop; and (3) a conserved CGAAAGGG on the 3' side of the gap.
van Keulen
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Department of Microbiology, State University of New York, Buffalo 14214.
Mertz
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LoVerde
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Shi
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Rekosh
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D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004275:DNA, Ribosomal / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000235:genetics; D015723:Gene Library; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012339:RNA, Ribosomal, 28S / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D012550:Schistosoma mansoni / Q000235:genetics*
D004275:DNA, Ribosomal; D004277:DNA, Single-Stranded; D012339:RNA, Ribosomal, 28S