[Measurement of anti-microsomal and anti-thyroglobulin antibodies by radioimmunoassay].
In this study, we measured anti-microsomal (same as anti-thyroid peroxidase) and anti-thyroglobulin antibodies (anti-TPOAb and anti-TgAb) in sera by new RIA kits and compared with the conventional particle agglutination (PA) kits. Two of 80 normals (2.5%) showed anti-TPOAb positive activity (greater than or equal to 0.3 unit/ml), and 9 of same samples (11.3%) showed anti TgAb positive activity (greater than or equal to 0.3 unit/ml). Sixty of 67 patients with Graves' disease (89.6%), and 14 of 22 with Hashimoto's disease (63.6%) were anti-TPOAb positive, and 42 (62.7%), and 18 (81.8%) were anti-TgAb positive respectively. Samples of 5 non-autoimmune disease were all negative by TPOAb and TgAb assays, suggesting the high specificity of new RIA kits to the autoimmune thyroid diseases. The RIA and PA methods showed a good correlation (anti-TPOAb:rs = 0.9134, anti-TgAb:rs = 0.7016). However, several negative sera in PA assay showed positive values in the RIA, so it was showed that new RIA kits were more sensitive than the PA assays.
Nakamura
H
H
Central Clinical Laboratories, Tokyo University School of Medicine.
Mikami
Y
Y
Aono
Y
Y
Yoshinoya
S
S
Uchimura
H
H
Okubo
A
A
Kitamura
M
M
Kubota
K
K
Sasaki
N
N
Takaku
F
F
D001323:Autoantibodies / Q000032:analysis*; D006801:Humans; D007453:Iodide Peroxidase / Q000276:immunology; D008861:Microsomes / Q000276:immunology*; D011863:Radioimmunoassay; D013954:Thyroglobulin / Q000276:immunology*
D001323:Autoantibodies; D013954:Thyroglobulin; D007453:Iodide Peroxidase