title: Quantitation of Fansimef components (mefloquine + sulfadoxine + pyrimethamine) in human plasma by two high-performance liquid chromatographic methods.
abstract: Two simple, precise, and selective high-performance liquid chromatographic methods are described for the simultaneous quantitation of mefloquine (MQ) plus pyrimethamine (PYR) or sulfadoxine (SDX) plus its principal metabolite N4-acetylsulfadoxine (N4SDX) in human plasma. After a single-step extraction, MQ plus PYR and SDX plus N4SDX including internal standards were separated using ion-paired and ion-suppression chromatography. Total run times for the assays were less than 12 min. Intraassay and interassay precision of the methods expressed as the coefficients of variation were less than 9% in plasma for the four compounds. The extraction recovery averaged 98% for MQ, 97% for PYR, 96% for SDX, and 81% for N4SDX. Plasma concentrations of the four compounds in a pediatric patient after a single oral dose of Fansimef (MQ + SDX + PYR) were determined to demonstrate the clinical application of the methods.
authors.affiliation: Department of Immunology and Biochemistry, Armed Forces Research Institute of Medical Sciences, San Francisco, CA 96346-5000.
authors.forename: M D
authors.initials: MD
authors.lastname: Edstein
authors.forename: I D
authors.initials: ID
authors.lastname: Lika
authors.forename: T
authors.initials: T
authors.lastname: Chongsuphajaisiddhi
authors.forename: A
authors.initials: A
authors.lastname: Sabchareon
authors.forename: H K
authors.initials: HK
authors.lastname: Webster
mesh_terms: D000962:Antimalarials / Q000493:pharmacokinetics*; D002648:Child; D002851:Chromatography, High Pressure Liquid; D004338:Drug Combinations; D006801:Humans; D008297:Male; D015767:Mefloquine / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics; D011739:Pyrimethamine / Q000097:blood* / Q000493:pharmacokinetics*; D013413:Sulfadoxine / Q000097:blood* / Q000493:pharmacokinetics*; D000096926:Benzenesulfonamides
chemical_list: D000962:Antimalarials; D004338:Drug Combinations; D015767:Mefloquine; D011739:Pyrimethamine; D013413:Sulfadoxine; C046846:mefloquine-sulfadoxine-pyrimethamine; D000096926:Benzenesulfonamides