The effect of an N-methyl-D-aspartate lesion in the hippocampus on glial and neuronal marker proteins.
The study employed an immunochemical quantification of brain cell marker proteins in addition to quantitative morphology in order to provide a more multifacetted and characterized model for an excitotoxic CNS lesion. The importance of the approach in the evaluation of the potential of neuroprotective agents is emphasized. The S-100 protein, the glial fibrillary acidic (GFA) protein, neuron specific enolase (NSE) and neuronal intermediary filament polypeptides (NF 68 and NF 200) were measured with a dot-immunobinding assay, 3-30 days after a unilateral injection of N-methyl-D-aspartate (NMDA) in the left dorsal hippocampus of the rat. After 3 days, the neuronal cell loss averaged 80% in the hippocampus. The S-100 content was reduced 3 days after injection, but was 150% of control at 30 days. GFA increased constantly from days 3 to 30. The neuronal marker proteins were all markedly reduced 7 days after injection. However, at 30 days, NF 68 and NF 200 were close to control (80%). Increasing content would reflect regeneration and sprouting of neurites. The content of the neuronal cytoplasmic marker, NSE, was significantly lower than control also at 10 and 30 days, although a gradual recovery could be traced.
Wang
S
S
Institute of Neurobiology, University of Göteborg, Sweden.
Lees
G J
GJ
Rosengren
L E
LE
Karlsson
J E
JE
Stigbrand
T
T
Hamberger
A
A
Haglid
K G
KG
D000818:Animals; D015415:Biomarkers; D002470:Cell Survival / Q000187:drug effects; D003593:Cytoplasm / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein / Q000276:immunology / Q000378:metabolism; D006624:Hippocampus / Q000033:anatomy & histology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D016202:N-Methylaspartate / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000276:immunology / Q000378:metabolism*; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009929:Organ Size / Q000187:drug effects; D010477:Perfusion; D010751:Phosphopyruvate Hydratase / Q000276:immunology / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D011919:Rats, Inbred Strains; D009418:S100 Proteins / Q000276:immunology / Q000378:metabolism
D015415:Biomarkers; D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; C030564:S-100 calcium-binding protein alpha subunit; D009418:S100 Proteins; D016202:N-Methylaspartate; D010751:Phosphopyruvate Hydratase