A quantitative microwell assay for chondrocyte cell adhesion.
An assay for measuring the quantity of live articular chondrocytes attached to a substratum in microwell plates was established by measuring the absorbance of the blue formazan product generated from the dye 3-(4,5-dimethylthiazol-2-yl)-2,5-dypenyl tetrazolium bromide (MTT). Blue formazan production was optimal at an MTT concentration of 1 mg/ml (100 microliters per microwell) and an incubation period of 3 h. The absorbance of the dye was linearly related to the quantity of cells added per microwell. The number of live chondrocytes attached to adhesive proteins can be quantitated using this technique.
Miller
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RR
Department of Musculoskeletal Research, Cleveland Clinic Foundation, Ohio 44195.
McDevitt
C A
CA
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology*; D002417:Cattle; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D003124:Colorimetry / Q000379:methods*; D004396:Coloring Agents; D005353:Fibronectins / Q000378:metabolism; D005562:Formazans / Q000378:metabolism; D007700:Kinetics; D008297:Male; D012756:Sheep; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013778:Tetrazolium Salts; D013844:Thiazoles
D004396:Coloring Agents; D005353:Fibronectins; D005562:Formazans; D013778:Tetrazolium Salts; D013844:Thiazoles; D013385:Succinate Dehydrogenase; C022616:thiazolyl blue