Quantitation of melphalan in plasma of patients by reversed-phase high-performance liquid chromatography with electrochemical detection.
An analytical procedure for the separation and determination of melphalan in human plasma was carried out. A simple high-performance liquid chromatographic method with electrochemical detection was developed taking advantage of the high sensitivity of the electrode redox reaction. The sample pretreatment consisted of a direct extraction of the interferents rather than of melphalan, owing to the difficulty of extraction of the drug, and was very simple, rapid and reproducible.
Silvestro
L
L
Istituto di Farmacologia e Terapia Sperimentale, Università di Torino, Italy.
Viano
I
I
Baiocchi
C
C
Saini
G
G
Marmont
F
F
Ferro
R
R
D000284:Administration, Oral; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D006801:Humans; D008558:Melphalan / Q000008:administration & dosage / Q000097:blood*; D010084:Oxidation-Reduction
D008558:Melphalan