Regional differences in catecholamine concentrations in bovine ovaries analysed by high-performance liquid chromatography.
Bovine corpora lutea and ovarian stroma were analysed by high-performance liquid chromatography for catecholamine content. High concentrations (up to 102 nmol/g wet weight) were found in both 'central' stroma, containing many blood vessels, and 'peripheral' stroma. Central stroma contained noradrenaline and some dopamine, whereas peripheral stroma contained a higher proportion of dopamine and also significant amounts of 3,4-dihydroxyphenylacetic acid (DOPAC). Occasional samples of stroma had very high amounts of dopamine, suggesting that it is stored in specific regions. Corpora lutea, although devoid of direct innervation, contained dopamine (up to 5.3 nmol/g) and noradrenaline (up to 1.2 nmol/g). The average dopamine:noradrenaline molar ratio was 1.19:1 and the concentrations of dopamine and noradrenaline were highly correlated (P less than 0.002). The concentration of dopamine was significantly higher in the early luteal phase of the oestrous cycle than during the rest of the cycle or in pregnancy. The levels of noradrenaline and dopamine present in corpora lutea are sufficient to modulate the production of both oxytocin and progesterone by luteal cells in vitro.
Denning-Kendall
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PA
Department of Anatomy, School of Medical Sciences, University of Bristol.
Wild
M L
ML
Wathes
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DC
D015102:3,4-Dihydroxyphenylacetic Acid / Q000032:analysis; D000818:Animals; D002417:Cattle / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003338:Corpus Luteum / Q000737:chemistry; D004298:Dopamine / Q000032:analysis*; D004971:Estrus / Q000378:metabolism; D005260:Female; D009638:Norepinephrine / Q000032:analysis*; D010053:Ovary / Q000737:chemistry*; D011374:Progesterone / Q000032:analysis
D015102:3,4-Dihydroxyphenylacetic Acid; D011374:Progesterone; D004298:Dopamine; D009638:Norepinephrine