Functional subpopulations of individual pancreatic B-cells in culture.
In the present study we have used a reverse hemolytic plaque assay and frequency distribution analysis to characterize the insulin secretory response of individual rat pancreatic B-cells in culture. Heterogeneous secretory behavior was observed with a multimodal distribution, suggesting the existence of B-cell subpopulations. Insulin secretion in response to increased extracellular glucose involved both amplification of secretion by individual cells as well as recruitment of previously silent B-cells.
Hiriart
M
M
Department of Neurosciences, Universidad Nacional Autonoma de Mexico, Mexico City.
Ramirez-Medeles
M C
MC
D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D003470:Culture Media; D005947:Glucose; D006462:Hemolytic Plaque Technique; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D009994:Osmolar Concentration
D003470:Culture Media; D007328:Insulin; D005947:Glucose