Studies on modulation of the effects of colchicine by L-cysteine using bone marrow of Swiss mice.
Colchicine (COL) elevates the frequency of micronucleated polychromatic erythrocytes (PE), the ratio of normochromatic to polychromatic erythrocytes (N/PE) and the frequency of large PE due to spindle disruption. Simultaneous i.p. injection of L-cysteine (CYS) does not influence the effects of COL while if administered 1 h prior to COL, CYS suppresses the N/PE ratio and frequency of large PE but not the frequency of micronucleated PE elevated by COL. Preincubation of CYS with COL at 37 degrees C for 1 h results in a significant decrease in all the COL effects. The modulatory effect of exogenous CYS appears to be due to its competition with the endogenous tubulin cysteine residues for interacting with COL.
Ghaskadbi
S
S
Department of Zoology, M.A.C.S. Research Institute, Pune, India.
Vaidya
V G
VG
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D002845:Chromatography; D002875:Chromosomes / Q000187:drug effects; D003078:Colchicine / Q000633:toxicity*; D003545:Cysteine / Q000494:pharmacology*; D004347:Drug Interactions; D004912:Erythrocytes / Q000187:drug effects; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D008941:Spindle Apparatus / Q000187:drug effects*; D014404:Tubulin / Q000378:metabolism
D014404:Tubulin; D003545:Cysteine; D003078:Colchicine