Changes in responsiveness of the canine basilar artery to endothelin-1 after subarachnoid hemorrhage.
The effect of endothelin-1 (ET-1) on the basilar arteries from control and subarachnoid hemorrhage (SAH) dogs were examined. The maximal contraction of the basilar artery in response to ET-1 was markedly decreased in the SAH group. Treatment with 10(-8)M phorbol 12-myristate 13-acetate (PMA) reduced the contractile responses to ET-1 in the basilar arteries from control dogs. ET-1-induced contractions of the basilar arteries from control dogs were similar to those in strips from SAH dogs by the treatment with 10(-8) M PMA. Ca(2+)-induced contraction of the basilar arteries which were depolarized with isotonic K+ (64 mM) were significantly attenuated in SAH dogs. Treatment with PMA also reduced the contractile responses to Ca2+ in the basilar arteries from control dogs. These results indicate that decreased contractile responses of the basilar arteries to ET-1 and Ca2+ in the SAH group may be related to changes in the activity of the protein kinase C in vascular smooth muscle.
Kamata
K
K
Department of Pharmacology, School of Pharmacy, Hoshi University, Tokyo, Japan.
Nishiyama
H
H
Miyata
N
N
Kasuya
Y
Y
D000818:Animals; D001488:Basilar Artery / Q000187:drug effects* / Q000503:physiopathology; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers; D004095:Dihydropyridines / Q000494:pharmacology; D004285:Dogs; D016232:Endothelins / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009529:Nicardipine / Q000494:pharmacology; D013345:Subarachnoid Hemorrhage / Q000503:physiopathology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D002121:Calcium Channel Blockers; D004095:Dihydropyridines; D016232:Endothelins; C054179:CD 349; D009529:Nicardipine; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium