title: Isolation of murine sialoglycoprotein using consecutive chromatography.
abstract: Affinity columns and high performance liquid chromatography were employed consecutively to obtain 89, 65, 46 and 29 kilodalton sialoglycoproteins from mouse erythrocyte ghosts free of the Band 3 protein which traditionally co-purifies with these proteins. The purification scheme involves Concanavalin A, Wheat Germ Agglutinin and/or Limulus lectin Sepharose 4B columns. We have designated these glycophorin-like proteins Sialoglycoproteins 1, 2, 3, and 4, respectively. Sialoglycoprotein 2 can be isolated independently using a Limulus column combination, while Sialoglycoproteins 3 and 4 were isolated separately during high performance liquid chromatography, demonstrating heterogeneity in binding properties between these sialoglycoproteins.
authors.affiliation: Division of Biomedical Sciences, School of Graduate Studies, Meharry Medical College, Nashville, Tennessee 37208.
authors.forename: D J
authors.initials: DJ
authors.lastname: Wilson
authors.forename: J M
authors.initials: JM
authors.lastname: Planas
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D003208:Concanavalin A / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000737:chemistry*; D006737:Horseshoe Crabs; D037102:Lectins / Q000737:chemistry; D051379:Mice; D012795:Sialoglycoproteins / Q000302:isolation & purification*; D014909:Wheat Germ Agglutinins / Q000737:chemistry
chemical_list: D037102:Lectins; D012795:Sialoglycoproteins; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A