Interleukin-4 induces the synthesis and secretion of MCP-1/JE by human endothelial cells.
The authors have demonstrated that human interleukin-4 (IL-4) induces increased expression of the mRNA encoding the monocyte-specific chemoattractant and activator, MCP-1/JE, in human endothelial cells (EC). In addition, treatment of ECs with IL-4 resulted in the synthesis and secretion of MCP-1/JE protein. While IL-4 did not significantly influence the induced expression of MCP-1/JE mRNA by interleukin-1 (IL-1) or tumor necrosis factor, concomitant treatment with IL-4 and IL-1 caused more secretion of MCP-1/JE protein than either cytokine alone. These results suggest that EC-produced MCP-1/JE may mediate some of IL-4's effects on monocyte physiology in vivo, including IL-4's anti-tumor properties.
Rollins
B J
BJ
Division of Medicine, Dana-Farber Cancer Institute, Boston, MA 02115.
Pober
J S
JS
D002478:Cells, Cultured; D018932:Chemokine CCL2; D002630:Chemotactic Factors / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D018932:Chemokine CCL2; D002630:Chemotactic Factors; D007375:Interleukin-1; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4