[The DNA-mediated transformation of mouse fibroblasts after radiation damage to the cellular chromatin].
In experiments with NIH3T3 mouse fibroblast the authors showed a dose-dependent (gamma- or UV-radiation) increase in the immortalized fibroblast transformation by purified DNA preparations from Ehrlich ascites tumour cells. The transformant yield was the highest when the transfection started within the first few minutes after irradiation, when radiation lesions occurred in the genome and the system of enzymic DNA repair was activated. The proteolytic activity inhibition by treating the exposed cells with phenylmethanesultonyl fluorine reduced the radiation-induced transformation.
Bezlepkin
V G
VG
Ostrovskaia
L B
LB
Bezlepkina
T A
TA
Giaziev
A I
AI
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000235:genetics; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D002478:Cells, Cultured / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D002843:Chromatin / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D004249:DNA Damage / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects*; D004260:DNA Repair / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D004273:DNA, Neoplasm / Q000302:isolation & purification / Q000494:pharmacology*; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D005720:Gamma Rays; D051379:Mice; D013997:Time Factors; D014162:Transfection / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D014466:Ultraviolet Rays
D002843:Chromatin; D004273:DNA, Neoplasm
DNK-oposredovannaia transformatsiia fibroblastov mysheĭ posle radiatsionnogo povrezhdeniia kletochnogo khromatina.