Molecular cloning and mapping of 10 new probes on the human Y chromosome.
We have developed a novel positive cloning vector whose use precludes the cloning of any fragments less than 0.8 kb as well as 3.4-kb EcoRI fragments of DYZ1, the largest repeating-DNA family on the long arm of the human Y chromosome. Using this vector, we subcloned inserts of a Y-chromosome-specific phage library constructed from EcoRI-digested flow-sorted Y-chromosome DNA. Ten novel Y-specific fragments were obtained. Their localization on the Y chromosome was determined by deletion mapping using clinical samples with structurally abnormal Y chromosomes. The long arm of the Y chromosome was divided into 12 segments by the novel probes in combination with established probes. The amelogenin-like sequence, mapped on the long arm in Human Gene Mapping 10, has been mapped on the short arm.
Nakahori
Y
Y
Department of Congenital Abnormalities Research, National Children's Medical Research Center, Tokyo, Japan.
Tamura
T
T
Nagafuchi
S
S
Fujieda
K
K
Minowada
S
S
Fukutani
K
K
Fuse
H
H
Hayashi
K
K
Kuroki
Y
Y
Fukushima
Y
Y
D053523:Amelogenin; D002460:Cell Line; D002869:Chromosome Aberrations / Q000235:genetics; D025063:Chromosome Disorders; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D015342:DNA Probes / Q000235:genetics*; D003746:Dental Enamel Proteins / Q000235:genetics; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D008297:Male; D010957:Plasmids / Q000235:genetics; D014998:Y Chromosome
D053523:Amelogenin; D015342:DNA Probes; D003746:Dental Enamel Proteins