title: Unusual immunologic properties of the uveitogenic interphotoreceptor retinoid-binding protein-derived peptide R23.
abstract: Peptide R23, consisting of residues 1091-1115 of bovine interphotoreceptor retinoid-binding protein (IRBP), had some unusual immunologic properties in Lewis rats. The peptide induced experimental autoimmune uveoretinitis and pinealitis in these rats, but only at high doses. The minimal immunopathogenic dose was found to be 100 nmol/rat. On the other hand, R23 was highly immunogenic in Lewis rats, producing cellular immunity, as measured by the lymphocyte proliferation assay, with a minimal dose of 1 nmol/rat. This unusual dissociation between the uveitogenic and immunogenic activities of R23 was attributed to different sites on the peptide, stimulating either the lymphocytes which induce disease or those which vigorously proliferate in culture. The potent immunogenicity of R23 was consistent with the peptide being immunodominant, as demonstrated by its capacity to be recognized by lymphocytes sensitized against whole IRBP and to stimulate these cells in culture to proliferate and acquire uveitogenic capacity. Likewise, lymphocytes sensitized against R23 are stimulated in culture by whole IRBP. Unexpectedly, peptide R23 was inferior to whole IRBP in its capacity to stimulate uveitogenicity in R23-sensitized lymphocytes. This finding also was attributed to the preferential stimulation by R23 of the lymphocytes specific for the putative "nonuveitogenic" site on the peptide. Peptide R23 also differs from the other tested bovine IRBP-derived peptides in the specificity of its antibodies. Unlike antibodies to the other peptides, those to R23 showed a strong cross reactivity toward whole IRBP.
authors.affiliation: Laboratory of Immunology, National Eye Institute, NIH, Bethesda, MD 20892.
authors.forename: S
authors.initials: S
authors.lastname: Kotake
authors.forename: T M
authors.initials: TM
authors.lastname: Redmond
authors.forename: B
authors.initials: B
authors.lastname: Wiggert
authors.forename: B
authors.initials: B
authors.lastname: Vistica
authors.forename: H
authors.initials: H
authors.lastname: Sanui
authors.forename: G J
authors.initials: GJ
authors.lastname: Chader
authors.forename: I
authors.initials: I
authors.lastname: Gery
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001323:Autoantibodies / Q000032:analysis; D003429:Cross Reactions; D005136:Eye Proteins / Q000276:immunology*; D007111:Immunity, Cellular; D016056:Immunodominant Epitopes; D016219:Immunotherapy, Adoptive; D008213:Lymphocyte Activation; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D011917:Rats, Inbred Lew; D012177:Retinol-Binding Proteins / Q000276:immunology*; D014605:Uveitis / Q000276:immunology* / Q000473:pathology; D000099214:Retinol-Binding Proteins, Interstitial
chemical_list: D001323:Autoantibodies; D005136:Eye Proteins; D016056:Immunodominant Epitopes; D012177:Retinol-Binding Proteins; D000099214:Retinol-Binding Proteins, Interstitial