Human melanoma cells transcribe interleukin 1 genes identical to those of monocytes.
Two molecular species of the pleotropic cytokine interleukin 1 (IL-1) are produced as products of two distinct genes transcribed by cells of the monocyte-macrophage lineage. We have shown previously that a significant proportion of human melanoma cell lines express IL-1 biological activity, but it has not been demonstrated that this activity is the same as authentic monocyte IL-1 alpha and -beta. Here we report the cloning and sequencing of IL-1 complementary DNAs from a metastatic melanoma cell line and demonstrate that they encode bona fide IL-1 alpha and IL-1 beta. In addition, IL-1 complementary DNAs encoding a different amino acid at position 145 were revealed.
Shabon
U
U
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104.
Bennicelli
J L
JL
Guerry
D P
DP
Koprowski
H
H
Ricciardi
R P
RP
D002460:Cell Line; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D015723:Gene Library; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D008545:Melanoma / Q000235:genetics* / Q000276:immunology; D009000:Monocytes / Q000276:immunology*; D009362:Neoplasm Metastasis; D014158:Transcription, Genetic
D004273:DNA, Neoplasm; D007375:Interleukin-1