Physiological, morphological, and physicochemical characterization of a novel Escherichia coli bacteriophage, phage MM.
A double-stranded DNA containing, T even-like, Escherichia coli bacteriophage, called MM, has been isolated from the local sewage and purified by polyethylene glycol precipitation followed by banding on a cesium chloride three-step gradient. It yields a burst size of 75 particles per infected cell, and has an adsorption coefficient of 3.3 x 10(-10) cm3/min and a latent period of 45 min. Electron microscopy of phage MM reveals an isometric icosahedral head, 92 nm long and 81 nm wide, and a 112-nm-long contractile tail with six pairs of 40-nm-long fibers attached to its baseplate. Phage MM appears similar to E. coli phage T4 or Salmonella phage O1. The density of phage MM in cesium chloride is 1.515 g/ml, and its total mass is 144 MDa. Gel electrophoresis of purified MM capsids displays two major capsid proteins in approximately equimolar amounts and with apparent molecular masses of 38 and 15 kDa. Similarly, purified MM tails yield two major polypeptides with apparent molecular masses of 55 and 16 kDa, most likely representing the major tail sheath and tail tube polypeptides. Its double-stranded DNA has a G-C content of 50%, a length of 131 kilobases (kb), and a mass of 89 MDa.
Müller
M
M
M. E. Müller-Institute for High-Resolution Electron Microscopy, Biocenter, University of Basel, Switzerland.
Wurtz
M
M
Kellenberger
E
E
Aebi
U
U
D000327:Adsorption; D002499:Centrifugation, Density Gradient; D003090:Coliphages / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure; D004279:DNA, Viral / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000254:growth & development; D007700:Kinetics; D008854:Microscopy, Electron; D010455:Peptides / Q000302:isolation & purification; D012722:Sewage; D013604:T-Phages / Q000502:physiology; D014764:Viral Proteins / Q000302:isolation & purification
D004279:DNA, Viral; D010455:Peptides; D012722:Sewage; D014764:Viral Proteins