Perspective and limitations of cryo-electron microscopy. From model systems to biological specimens.
We investigated the possibility of vitrifying temperature-sensitive lipid phases as well as (small) biological specimens. From a suspension of unilamellar vesicles, prepared from dipalmitoyl-phosphatidylcholine (DPPC), thin aqueous films were formed at various temperatures. With cryo-electron microscopy vesicles were found to be smooth, rippled and faceted or faceted only, depending on the temperature of thin-film formation (318, 312 and 296 K respectively). The morphology and the electron diffraction patterns indicate that membranes can by physically fixed by vitrification in their high-temperature configuration and studied at low temperature by cryo-electron microscopy. This finding suggests that it may also be possible to preserve, in their original state, the more complex membrane systems found in living organisms by initiating rapid-cooling at a physiological temperature. This was explored by vitrification of thin films formed on specimen grids with (human) blood platelets adhering to collagen fibres. Low-temperature observation with an acceleration voltage of 120 kV revealed subcellular details, More details were observed when using higher accelerating voltages (200 and 300 kV) of the electron beam. The results presented in this paper illustrate the great potential of cryo-electron microscopy in the study of membrane dynamics, both in relatively simple model membrane systems and in more complex biological membrane systems.
Frederik
P M
PM
Department of Pathology, University of Limburg, Maastricht, The Netherlands.
Stuart
M C
MC
Bomans
P H
PH
Busing
W M
WM
Burger
K N
KN
Verkleij
A J
AJ
D001792:Blood Platelets / Q000648:ultrastructure*; D002448:Cell Adhesion; D002462:Cell Membrane / Q000648:ultrastructure; D003094:Collagen / Q000648:ultrastructure; D005629:Frozen Sections; D006801:Humans; D008854:Microscopy, Electron / Q000379:methods*; D010743:Phospholipids
D010743:Phospholipids; D003094:Collagen