Effects of dexamethasone on the activity of histidine decarboxylase, ornithine decarboxylase, and dopa decarboxylase in rat oxyntic mucosa.
Since accelerated turnover of histamine in oxyntic mucosa may be an important factor in the pathogenesis of peptic ulcers, the effect of dexamethasone and other glucocorticoids on the activity of gastric histidine decarboxylase (HDC) was studied in the rat. The activity of HDC in rat oxyntic mucosa increased significantly after dexamethasone was injected s.c. to rats at doses larger than 0.4 mg/kg body weight. The maximum response of the HDC activity to dexamethasone (4 mg/kg) was observed 8 h after the treatment. The activity of ornithine decarboxylase (ODC) increased at 4 h, while that of DOPA decarboxylase showed no significant change throughout the 16-h period following a single injection of dexamethasone. The mucosal levels of histamine, putrescine, and spermidine rose significantly after the steroid treatment, while the spermine levels remained nearly constant. There was no sex difference in these responses to dexamethasone. Betamethasone showed nearly the same effects as dexamethasone on the decarboxylase activities and the mucosal levels of diamines. Serum gastrin levels showed no significant change for the first 4 h and then rose significantly 8 and 16 h after dexamethasone treatment. Pentagastrin (0.5 mg/kg) increased the HDC activity, while it showed no significant effect on either the mucosal ODC activity or levels of polyamines and histamine. These data suggest that dexamethasone influences the metabolism of histamine and polyamines in rat oxyntic mucosa both directly and via stimulation of gastrin release.
Araki
M
M
First Department of Surgery, Gunma University School of Medicine, Maebashi, Japan.
Nakamura
M
M
Takenoshita
S
S
Shoda
H
H
Nagamachi
Y
Y
Matsuzaki
S
S
D000818:Animals; D001623:Betamethasone / Q000494:pharmacology; D015317:Biogenic Polyamines / Q000378:metabolism; D002262:Carboxy-Lyases / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology*; D004296:Dopa Decarboxylase / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects / Q000201:enzymology*; D005755:Gastrins / Q000097:blood; D006632:Histamine / Q000378:metabolism; D006640:Histidine Decarboxylase / Q000378:metabolism; D008297:Male; D009955:Ornithine Decarboxylase / Q000378:metabolism; D010418:Pentagastrin / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D015317:Biogenic Polyamines; D005755:Gastrins; D003907:Dexamethasone; D006632:Histamine; D001623:Betamethasone; D002262:Carboxy-Lyases; D004296:Dopa Decarboxylase; D009955:Ornithine Decarboxylase; D006640:Histidine Decarboxylase; D010418:Pentagastrin