title: Effect of essential fatty acid deficiency on lipid composition of basolateral plasma membrane of pig intestinal mucosal cells.
abstract: The effect of essential fatty acid (EFA) deficiency on the lipid composition of basolateral plasma membranes (BPM) from intestinal mucosal cells was investigated in weaning pigs fed control or EFA-deficient diets for 12 weeks. The phospholipid and cholesterol contents relative to protein were similar in both groups, showing a cholesterol/phospholipid molar ratio of 0.6. The distribution of phospholipid classes was also unaffected by the diet. In contrast, fatty acid profiles of the two phospholipid main classes, phosphatidylcholine and phosphatidyl-ethanolamine were altered by EFA deficiency. Linoleic acid (18:2n-6) was largely reduced, whereas arachidonic acid (20:4n-6) only slightly decreased in EFA-deficient pigs. The unsaturation index was essentially maintained by high levels of oleic acid (18:1n-9) and by conversion of oleic acid to 5,8,11-eicosatrienoic acid (20:3n-9). Finally, during the period of EFA deficiency, the lipid composition of BPM of the intestinal mucosal cells was little affected, suggesting a preferential uptake of 20:4n-6 and (or) precursor mobilized from other tissues. However, an effect of dietary treatment on the function of membrane-associated proteins cannot be ruled out.
authors.affiliation: Laboratoire de Nutrition et Securité Alimentaire, CRJ-INRA, Jouy-en-Josas, France.
authors.forename: V
authors.initials: V
authors.lastname: Duranthon
authors.forename: L
authors.initials: L
authors.lastname: Frémont
authors.forename: C L
authors.initials: CL
authors.lastname: Léger
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D002784:Cholesterol / Q000032:analysis; D002849:Chromatography, Gas; D004032:Diet; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000032:analysis*; D005228:Fatty Acids, Essential / Q000172:deficiency*; D007413:Intestinal Mucosa / Q000737:chemistry*; D008055:Lipids / Q000032:analysis*; D008297:Male; D010713:Phosphatidylcholines / Q000032:analysis; D010714:Phosphatidylethanolamines / Q000032:analysis; D013552:Swine
chemical_list: D005227:Fatty Acids; D005228:Fatty Acids, Essential; D008055:Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D002784:Cholesterol