Mutations permitting the anaerobic growth of Escherichia coli on trehalose.
Wild-type strains of Escherichia coli K-12 do not grow anaerobically on trehalose or galactose. We isolated two operon fusion mutants of E. coli which gained the ability to grow on trehalose anaerobically (tan). The tanA-lac mutation was located at 41 min on the E. coli genetic map and also abolished growth on glucuronic acid both aerobically and anaerobically. The tanB-lac mutation was mapped to 68 min and permitted anaerobic growth on galactose as well as trehalose. The tanB-lac fusion was induced anaerobically whereas tanA-lac showed more or less constitutive beta-galactosidase expression.
Mat-Jan
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F
Department of Microbiology, Southern Illinois University, Carbondale 62901.
Williams
C R
CR
Clark
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DP
D000693:Anaerobiosis; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D009154:Mutation; D009876:Operon; D014161:Transduction, Genetic; D014199:Trehalose / Q000378:metabolism*
D004269:DNA, Bacterial; D014199:Trehalose; D005690:Galactose