Quantification of glial fibrillary acidic protein in developing sheep brain cortex, cerebellum and stem.
1. The glial fibrillary acidic protein (GFAP) content of foetal, young (lamb) and adult sheep brain white (stem and cerebellum) and grey (cortex) matter-enriched regions has been determined by means of an improved ELISA using one layer of anti-human GFAP monoclonal antibody. 2. The order of GFAP concentration in brain regions was as follows: brain stem greater than cerebellum greater than cortex. 3. Postnatal brain development accounts for an increase of GFAP in all the regions. The most important increase in GFAP was observed in the adult brain and was proportionally more significant in the grey matter-enriched cortex.
Andrés-Barquín
P J
PJ
Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Universidad de Zaragoza, Spain.
Pérez-Martos
A
A
López-Pérez
M J
MJ
D000375:Aging / Q000378:metabolism; D000818:Animals; D001933:Brain Stem / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism; D002531:Cerebellum / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism; D002540:Cerebral Cortex / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D012756:Sheep / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism
D005904:Glial Fibrillary Acidic Protein