Protein growth factor(s) from C6 glioma cells that promote the growth of murine hybridomas.
C6 glioma cell conditioned medium (C6CM) has been used as a growth supplement for murine hybridomas. At 10% C6CM has been found to increase cell proliferation by 3-4-fold. This effect is observed in the presence of saturating concentration of FCS. Clonal growth is also enhanced 6.7-fold. No growth promoting effect is seen on murine myelomas or spleen cells. The factor(s) appear to be protein in nature.
Gomathi
K G
KG
Department of Biochemistry, All India Institute of Medical Sciences, New Delhi.
Sharma
E
E
Sharma
S K
SK
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D005910:Glioma / Q000503:physiopathology*; D006133:Growth Substances / Q000494:pharmacology*; D006825:Hybridomas / Q000166:cytology / Q000187:drug effects*; D015850:Interleukin-6 / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D011506:Proteins / Q000494:pharmacology*; D051381:Rats
D003470:Culture Media; D006133:Growth Substances; D015850:Interleukin-6; D011506:Proteins