title: Purification and characterization of a 1,2-dihydroxynaphthalene dioxygenase from a bacterium that degrades naphthalenesulfonic acids.
abstract: 1,2-Dihydroxynaphthalene dioxygenase was purified to homogeneity from a bacterium that degrades naphthalenesulfonic acids (strain BN6). The enzyme requires Fe2+ for maximal activity and consists of eight identical subunits with a molecular weight of about 33,000. Analysis of the NH2-terminal amino acid sequence revealed a high degree of homology (22 of 29 amino acids) with the NH2-terminal amino acid sequence of 2,3-dihydroxybiphenyl dioxygenase from strain Pseudomonas paucimobilis Q1. 1,2-Dihydroxynaphthalene dioxygenase from strain BN6 shows a wide substrate specificity and also cleaves 5-, 6-, and 7-hydroxy-1,2-dihydroxynaphthalene, 2,3- and 3,4-dihydroxybiphenyl, catechol, and 3-methyl- and 4-methylcatechol. Similar activities against the hydroxy-1,2-dihydroxynaphthalenes were also found in cell extracts from naphthalene-degrading bacteria.
authors.affiliation: Institut für Mikrobiologie der Universität Stuttgart, Germany.
authors.forename: A E
authors.initials: AE
authors.lastname: Kuhm
authors.forename: A
authors.initials: A
authors.lastname: Stolz
authors.forename: K L
authors.initials: KL
authors.lastname: Ngai
authors.forename: H J
authors.initials: HJ
authors.lastname: Knackmuss
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology* / Q000235:genetics; D001673:Biodegradation, Environmental; D049308:Dioxygenases; D004790:Enzyme Induction; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009282:Naphthalenesulfonates / Q000378:metabolism*; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013056:Spectrophotometry, Ultraviolet; D013379:Substrate Specificity
chemical_list: D009282:Naphthalenesulfonates; D010105:Oxygenases; C023133:dihydroxynaphthalene oxygenase; D049308:Dioxygenases; C074272:2,3-dihydroxybiphenyl oxygenase