Change of intracellular calcium of neural cells induced by extracellular ATP.
Exposure of various neural cells to ATP increased intracellular Ca2+ and the production of inositol trisphosphate. The Ca2+ responses were also observed in the absence of extracellular Ca2+, suggesting that a part of Ca2+ mobilization took place from cytosolic storage. Since adenosine had no effect on intracellular Ca2+ increment, ATP appears to act through a P2-purinergic receptor. Islet-activating protein or pertussis toxin pretreatment hardly influenced the increase in intracellular Ca2+ and inositol trisphosphate production induced by ATP, suggesting that IAP-sensitive GTP-binding proteins do not play a practical role in this reaction.
Hirano
Y
Y
Institute of Endocrinology, Gunma University, Maebashi, Japan.
Okajima
F
F
Tomura
H
H
Majid
M A
MA
Takeuchi
T
T
Kondo
Y
Y
D000241:Adenosine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004789:Enzyme Activation; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D007295:Inositol Phosphates / Q000096:biosynthesis; D051379:Mice; D009447:Neuroblastoma / Q000378:metabolism*; D037342:Pertussis Toxin; D010673:Pheochromocytoma / Q000378:metabolism*; D051381:Rats; D014407:Tumor Cells, Cultured; D010738:Type C Phospholipases / Q000378:metabolism; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D007295:Inositol Phosphates; D010566:Virulence Factors, Bordetella; D000255:Adenosine Triphosphate; D037342:Pertussis Toxin; D010738:Type C Phospholipases; D000241:Adenosine; D002118:Calcium