Use of ultrasound to increase infection of McCoy cell monolayers by Chlamydia trachomatis strain.
An ultrasound cell disrupter with a cooled cup tip was used to increase rapidly Chlamydia trachomatis infection in vitro. After three growth cycles of the NI-1 strain (serovar E), the pulsed ultrasound use enhanced the number of infected McCoy cells by approximately 12-times, as compared with control; and 8.8-times over the shaking with glass beads and centrifugation technique. After three growth cycles of the fast-growing LB-1 strain (serovar L2), the enhancement was by 15 and 10.8 respectively. Consequently, ultrasound treatment with a cooled cup tip can offer a working standard procedure to increase rapidly the number of cells infected with Chlamydia.
Moncan
T
T
Laboratoire de Bacteriologie et Immunologie générale, C.H.R.U., Amiens, France.
Eb
F
F
Orfila
J
J
D001431:Bacteriological Techniques; D002478:Cells, Cultured; D002692:Chlamydia trachomatis / Q000254:growth & development* / Q000302:isolation & purification; D006801:Humans; D014465:Ultrasonics