A hybrid cell mapping panel for regional localization of probes to human chromosome 8.
We have characterized a panel of somatic cell hybrids that carry fragments of human chromosome 8 and used this panel for the regional localization of anonymous clones derived from a chromosome 8 library. The hybrid panel includes 11 cell lines, which were characterized by Southern blot hybridization with chromosome 8-specific probes of known map location and by fluorescent in situ hybridization with a probe derived from a chromosome 8 library. The chromosome fragments in the hybrid cell lines divide the chromosome into 10 intervals. Using this mapping panel, we have mapped 56 newly derived anonymous clones to regions of chromosome 8. We have also obtained physical map locations for 7 loci from the genetic map of chromosome 8, thus aligning the genetic and physical maps of the chromosome.
Wagner
M J
MJ
Institute for Molecular Biology, University of Houston, Texas 77204.
Ge
Y
Y
Siciliano
M
M
Wells
D E
DE
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping / Q000379:methods*; D002898:Chromosomes, Human, Pair 8; D003001:Cloning, Molecular; D006224:Cricetinae; D015342:DNA Probes / Q000235:genetics; D005453:Fluorescence; D015723:Gene Library; D006801:Humans; D006822:Hybrid Cells; D009693:Nucleic Acid Hybridization
D015342:DNA Probes