cDNA cloning and chromosomal localization of the human beta-adrenergic receptor kinase.
The beta-adrenergic receptor kinase (beta ARK) mediates agonist-dependent phosphorylation of the beta 2-adrenergic and related G protein-coupled receptors. A cDNA encoding bovine beta ARK has recently been isolated. In this work we have isolated a cDNA encoding human beta ARK from a retinal cDNA library. The cDNA encodes a protein of 689 amino acids with an overall 98.0% amino acid and 92.5% nucleotide identity with bovine beta ARK. Chromosomal location of the human beta ARK gene was determined by correlating the presence of the beta ARK locus with retention of a specific human chromosome in a rodent-human hybrid panel. This analysis revealed that the human beta ARK locus segregated with the long arm of chromosome 11, centromeric to 11q13.
Benovic
J L
JL
Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, PA 19140.
Stone
W C
WC
Huebner
K
K
Croce
C
C
Caron
M G
MG
Lefkowitz
R J
RJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D003001:Cloning, Molecular; D017868:Cyclic AMP-Dependent Protein Kinases; D004247:DNA / Q000235:genetics*; D054769:G-Protein-Coupled Receptor Kinase 2; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000235:genetics*; D012377:Rodentia; D051552:beta-Adrenergic Receptor Kinases
D004247:DNA; D011494:Protein Kinases; D017868:Cyclic AMP-Dependent Protein Kinases; C496567:GRK2 protein, human; D051552:beta-Adrenergic Receptor Kinases; D054769:G-Protein-Coupled Receptor Kinase 2