title: gp160, the envelope glycoprotein of human immunodeficiency virus type 1, is a dimer of 125-kilodalton subunits stabilized through interactions between their gp41 domains.
abstract: The molecular masses, carbohydrate contents, oligomeric status, and overall molecular structure of the env glycoproteins of human immunodeficiency virus type 1--gp120, gp160, and gp41--have been determined by quantitative electron microscopy. Using purified gp160s, a water-soluble form of env purified from a recombinant vaccinia virus expression system, we have measured the masses of several hundred individual molecules by dark-field scanning transmission electron microscopy. When combined with sequence-based information, these mass measurements establish that gp160s is a dimer of subunits with an average monomer mass of 123 kDa, of which approximately 32 kDa is carbohydrate and 91 kDa is protein. Similarly, gp120 was found to be a monomer of 89 kDa and to contain virtually all of env's glycosylation. gp41 is glycosylated only slightly, if at all, and is responsible for the interactions that stabilize the gp160s dimer. A molecular mass map of gp160s derived by image processing depicts an asymmetric dumbbell whose two domains have masses of approximately 173 and approximately 73 kDa, corresponding to a gp120 dimer and a gp41 dimer, respectively. We infer that the average monomer mass of native gp160 is 125 kDa and that in situ, env is either a dimer or a tetramer but is most unlikely to be a trimer.
authors.affiliation: Laboratory of Structural Biology Research, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Bethesda, Maryland 20892.
authors.forename: D J
authors.initials: DJ
authors.lastname: Thomas
authors.forename: J S
authors.initials: JS
authors.lastname: Wall
authors.forename: J F
authors.initials: JF
authors.lastname: Hainfeld
authors.forename: M
authors.initials: M
authors.lastname: Kaczorek
authors.forename: F P
authors.initials: FP
authors.lastname: Booy
authors.forename: B L
authors.initials: BL
authors.lastname: Trus
authors.forename: F A
authors.initials: FA
authors.lastname: Eiserling
authors.forename: A C
authors.initials: AC
authors.lastname: Steven
mesh_terms: D015686:Gene Products, env / Q000737:chemistry / Q000648:ultrastructure*; D015699:HIV Envelope Protein gp120 / Q000737:chemistry / Q000648:ultrastructure*; D019302:HIV Envelope Protein gp160; D015700:HIV Envelope Protein gp41 / Q000737:chemistry / Q000648:ultrastructure*; D015497:HIV-1 / Q000648:ultrastructure*; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D015394:Molecular Structure; D008970:Molecular Weight; D011487:Protein Conformation; D011498:Protein Precursors / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D015686:Gene Products, env; D015699:HIV Envelope Protein gp120; D019302:HIV Envelope Protein gp160; D015700:HIV Envelope Protein gp41; D046911:Macromolecular Substances; D011498:Protein Precursors