title: Localization of metallothionein in nuclei of growing primary cultured adult rat hepatocytes.
abstract: In primary cultured adult rat hepatocytes stimulated by epidermal growth factor and insulin, dramatic changes in the subcellular distribution of metallothionein were clarified by indirect immunofluorescence using antisera specific for this protein. Metallothionein was detected only in the cytoplasm of cultured hepatocytes in the G0 and G1 phases, but was concentrated in the cell nuclei in the early S phase. The strongest staining pattern in the nuclei was observed 12 h after stimulation. Subsequently, the intensity of metallothionein staining in the nuclei decreased. These results suggest that primary cultured hepatocytes are suitable for examining the relation between subcellular localization of metallothionein and cell growth.
authors.affiliation: Faculty of Pharmaceutical Sciences, Osaka University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tsujikawa
authors.forename: T
authors.initials: T
authors.lastname: Imai
authors.forename: M
authors.initials: M
authors.lastname: Kakutani
authors.forename: Y
authors.initials: Y
authors.lastname: Kayamori
authors.forename: T
authors.initials: T
authors.lastname: Mimura
authors.forename: N
authors.initials: N
authors.lastname: Otaki
authors.forename: M
authors.initials: M
authors.lastname: Kimura
authors.forename: R
authors.initials: R
authors.lastname: Fukuyama
authors.forename: N
authors.initials: N
authors.lastname: Shimizu
mesh_terms: D000818:Animals; D000906:Antibodies; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008668:Metallothionein / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013936:Thymidine / Q000378:metabolism
chemical_list: D000906:Antibodies; D007328:Insulin; D004815:Epidermal Growth Factor; D008668:Metallothionein; D013936:Thymidine