title: Dipyrone enhances intracellular accumulation and cytotoxicity of adriamycin in human chronic myeloid leukemia cells.
abstract: The cytotoxicity induced by dipyrone alone, or in combination with adriamycin (ADR) was studied in human chronic myeloid leukemia (CML) cells. The inhibition of 3H-thymidine incorporation into DNA was taken as a measure of cytotoxicity. While dipyrone alone indicated marginal inhibition, its combination with ADR demonstrated a potentiating effect (p less than 0.001), which was found to be irreversible. The enhanced cytotoxicity of the combination was a result of an increased drug accumulation as studied by the uptake of 14C-adriamycin. Observations indicate that dipyrone can be used to enhance the cytotoxicity of ADR in human CML patients.
authors.affiliation: Cellular Chemotherapy Unit, Tata Memorial Center, Parel, Bombay, India.
authors.forename: M P
authors.initials: MP
authors.lastname: Chitnis
authors.forename: N S
authors.initials: NS
authors.lastname: Kamath
authors.forename: H K
authors.initials: HK
authors.lastname: Parekh
authors.forename: S H
authors.initials: SH
authors.lastname: Advani
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D004177:Dipyrone / Q000494:pharmacology*; D004317:Doxorubicin / Q000378:metabolism / Q000627:therapeutic use*; D004353:Drug Evaluation, Preclinical; D004357:Drug Synergism; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000188:drug therapy* / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D004177:Dipyrone; D004317:Doxorubicin; D004247:DNA; D013936:Thymidine