title: High-performance liquid chromatographic analysis of the (cyanoaquo) stereoisomers of several putative vitamin B12 precursors.
abstract: The cyanoaquo and aquocyano stereoisomers of several putative vitamin B12 precursors are reversibly formed and can be separated using analytical high-performance liquid chromatographic methods. The behavior of these stereoisomers varies somewhat depending on the type of column used and the chromatographic conditions employed. Both reversed-phase and ion-exchange columns can be used to observe the reversible formation and separation of the stereoisomers of (H2O,CN)cobyric acid, cobinamide and the cobinic acid pentaamide-1, -2 and -3 structural isomers. The greatest differences in retention times are seen when the pH of the eluting buffer is less than 4.0 and the buffer contains no KCN.
authors.affiliation: Chicago State University, Department of Chemistry and Physics, IL 60628.
authors.forename: S H
authors.initials: SH
authors.lastname: Ford
authors.forename: J
authors.initials: J
authors.lastname: Gallery
authors.forename: A
authors.initials: A
authors.lastname: Nichols
authors.forename: M
authors.initials: M
authors.lastname: Shambee
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003038:Cobamides / Q000032:analysis*; D045728:Corrinoids; D006863:Hydrogen-Ion Concentration; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D014805:Vitamin B 12 / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism*
chemical_list: D003038:Cobamides; D045728:Corrinoids; C017142:cobinamide; C012851:cobyric acid; D014805:Vitamin B 12