title: Comparison of the humoral and cytotoxic T-lymphocyte response to individual HLA class I alloantigens in highly immunized patients.
abstract: In order to investigate the correlation between activation of cytotoxic T-lymphocyte precursor (CTLp) and the formation of antibodies to alloantigens, we studied 21 highly sensitized patients waiting for a kidney transplantation. Both antibody reactivity and CTLp frequencies of these patients were determined against 88 individual HLA class I alloantigens. A high or low CTLp frequency against a certain HLA-A or -B alloantigen was not correlated with the presence or absence of the antibodies to that antigen. Mismatched antigens, towards which the patient had not formed antibodies, can induce either a higher or a lower frequency of CTLp compared to mismatches towards which the patients had formed antibodies. The possible implications of this lack of correlation between the T- and B-cell allorepertoires with regard to donor selection for (highly immunized) patients is discussed.
authors.affiliation: Department of Immunohaematology and Blood Bank, University Hospital Leiden, The Netherlands.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: van Bree
authors.forename: Y
authors.initials: Y
authors.lastname: Gijbels
authors.forename: A
authors.initials: A
authors.lastname: van Veen
authors.forename: J J
authors.initials: JJ
authors.lastname: van Rood
authors.forename: F H
authors.initials: FH
authors.lastname: Class
mesh_terms: D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D006412:Hematopoietic Stem Cells / Q000276:immunology; D006801:Humans; D007114:Immunization; D007518:Isoantibodies / Q000096:biosynthesis; D016030:Kidney Transplantation / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014181:Transplantation Immunology
chemical_list: D015234:HLA-A Antigens; D015235:HLA-B Antigens; D007518:Isoantibodies