Vitamin E adjuvant formulations in mice.
Water-in-oil-type adjuvants prepared with natural or synthetic vitamin E as the oil phase, Arlacel A or Montanide 103 as emulsifier and 1.0% aqueous solution of bovine serum albumin (BSA) as antigen were tested in mice. An emulsion of 0.42 ml vitamin E, 0.42 ml special mineral oil, 0.15 ml Arlacel A and 1.0 ml BSA gave maximal humoral response, but 0.85 ml special mineral oil, 0.15 ml Arlacel A and 1.0 ml BSA (standard Freund's incomplete adjuvant formulation) gave the greatest delayed-type hypersensitivity response.
Tengerdy
R P
RP
Department of Microbiology, Colorado State University, Fort Collins 80523.
Lacetera
N G
NG
D000276:Adjuvants, Immunologic / Q000737:chemistry*; D000818:Animals; D000917:Antibody Formation; D004121:Dimethyl Sulfoxide; D004655:Emulsions; D005260:Female; D005620:Freund's Adjuvant; D006968:Hypersensitivity, Delayed; D008297:Male; D051379:Mice; D008899:Mineral Oil; D012710:Serum Albumin, Bovine / Q000276:immunology; D024505:Tocopherols; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology*; D024502:alpha-Tocopherol / Q000031:analogs & derivatives*
D000276:Adjuvants, Immunologic; D004655:Emulsions; D014810:Vitamin E; D012710:Serum Albumin, Bovine; D008899:Mineral Oil; D005620:Freund's Adjuvant; D024502:alpha-Tocopherol; D024505:Tocopherols; D004121:Dimethyl Sulfoxide