title: Characteristics of histamine-induced inositol phospholipid hydrolysis in HeLa cells.
abstract: HeLa cells, a human epithelial carcinoma, contain Histamine H1-receptors, as defined by [3H] mepyramine binding studies. Histamine, via H1-receptors, stimulates the production of inositol phosphates (IP) with an EC50 of 1.2 microM. Preincubation of HeLa cells with histamine, followed by a further histamine challenge results in a slower time course of [3H] IP production, suggesting the occurrence of H1-receptor desensitisation.
authors.affiliation: Department of Pharmacology, University of Cambridge, United Kingdom.
authors.forename: D R
authors.initials: DR
authors.lastname: Bristow
authors.forename: J M
authors.initials: JM
authors.lastname: Young
mesh_terms: D002217:Carbachol / Q000494:pharmacology; D006367:HeLa Cells; D006632:Histamine / Q000494:pharmacology*; D006801:Humans; D006868:Hydrolysis; D007295:Inositol Phosphates / Q000378:metabolism*; D007700:Kinetics; D011738:Pyrilamine / Q000378:metabolism; D011969:Receptors, Histamine H1 / Q000187:drug effects / Q000378:metabolism
chemical_list: D007295:Inositol Phosphates; D011969:Receptors, Histamine H1; D006632:Histamine; D002217:Carbachol; D011738:Pyrilamine