title: Subconductance states in calcium-activated potassium channels from canine airway smooth muscle.
abstract: The single-channel patch clamp technique was used to analyze subconductance states in the 260 pS calcium-activated potassium channel from canine airway smooth muscle. More than sixty minutes of single channel data (greater than 87,000 events) from five excised patches were analyzed. Six subconductance amplitudes were clearly established to be 17, 33, 41, 52, 63 and 72% of the full conductance. Subconductance openings were usually brief (milliseconds) and represented less than 5% of the total channel open time, but they also persisted for several seconds on rare occasions. They appeared to be unaffected by voltage or time after seal formation, but may have increased in occurrence with decreasing calcium concentration. Irregular amplitude intervals, and the presence of ramp-like, analog transitions between conductance states, suggest a model for maxi-K subconductance states in which the channel protein undergoes random conformational changes causing a variable pore size.
authors.affiliation: Department of Physiology, University of Alberta, Edmonton, Canada.
authors.forename: L L
authors.initials: LL
authors.lastname: Stockbridge
authors.forename: A S
authors.initials: AS
authors.lastname: French
authors.forename: S F
authors.initials: SF
authors.lastname: Man
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002478:Cells, Cultured; D004285:Dogs; D008564:Membrane Potentials / Q000187:drug effects*; D008954:Models, Biological; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism*; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism*; D014132:Trachea / Q000187:drug effects / Q000378:metabolism*
chemical_list: D015221:Potassium Channels; D002118:Calcium