title: A versatile solid support system for oligodeoxynucleotide probe-based hybridization assays.
abstract: A procedure for immobilization of well-defined quantities of oligodeoxyribonucleotides (ODNs) to a versatile nylon support is described. The solid support, a nylon-6/6 bead, is covalently coated with poly(ethyleneimine) to provide a reactive spacer-arm for attachment of ODNs. 5'-Aminohexyl-tailed ODNs are selectively activated using 2,4,6-trichloro-1,3,5-triazine (cyanuric chloride) and then covalently attached to the bead via the triazine moiety. The modified nylon support has a low level of binding of nonspecific nucleic acid and efficiently captures both RNA and DNA targets.
authors.affiliation: MicroProbe Corporation, Bothell, WA 98021.
authors.forename: J
authors.initials: J
authors.lastname: Van Ness
authors.forename: S
authors.initials: S
authors.lastname: Kalbfleisch
authors.forename: C R
authors.initials: CR
authors.lastname: Petrie
authors.forename: M W
authors.initials: MW
authors.lastname: Reed
authors.forename: J C
authors.initials: JC
authors.lastname: Tabone
authors.forename: N M
authors.initials: NM
authors.lastname: Vermeulen
mesh_terms: D000478:Alkylation; D001483:Base Sequence; D003124:Colorimetry; D004247:DNA; D005453:Fluorescence; D005821:Genetic Techniques; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009757:Nylons; D015345:Oligonucleotide Probes; D011094:Polyethyleneimine; D014227:Triazines
chemical_list: D009757:Nylons; D015345:Oligonucleotide Probes; D014227:Triazines; C019309:cyanuric chloride; D011094:Polyethyleneimine; D004247:DNA