Effects of amidated rat islet amyloid polypeptide on glucose-stimulated insulin secretion in vivo and in vitro in rats.
Amidated rat islet amyloid polypeptide (IAPP) is a constituent of secretory granules in islet B-cells. We examined its influence on glucose-stimulated insulin secretion in rats. In vivo, intravenous infusion of IAPP (68 pmol/min) did not affect glucose-stimulated insulin secretion. In vitro, IAPP slightly inhibited glucose (8.3 mM)-stimulated insulin secretion from isolated rat islets at the high dose level of 10(-5) M but not at the lower dose levels. We conclude that the IAPP is not involved in the normal regulation of insulin secretion.
Ar'Rajab
A
A
Department of Surgery, Lund University, Sweden.
Ahrén
B
B
D000577:Amides / Q000494:pharmacology; D000682:Amyloid / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D005947:Glucose / Q000494:pharmacology*; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D058228:Islet Amyloid Polypeptide; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D000577:Amides; D000682:Amyloid; D007328:Insulin; D058228:Islet Amyloid Polypeptide; D005947:Glucose