Antioxidant properties of the alkaloid boldine in systems undergoing lipid peroxidation and enzyme inactivation.
Boldine, in low micromolar concentrations, was able to prevent brain homogenate autooxidation, the 2,2'-azobis(2-amidinopropane)(AAP)-induced lipid peroxidation of red cell plasma membranes, and the AAP-induced inactivation of lysozyme. These results are indicative of a high reactivity of boldine towards free radicals. The analysis of the boldine effect as a function of incubation times suggests that a metabolite resulting from the interaction of boldine with free radicals also exhibits antioxidant activity, being more efficient than boldine in brain homogenate auto-oxidation and less efficient in lysozyme protection experiments. This behavior may be accounted for in terms of the relative location of the scavengers needed to afford maximal protection.
Speisky
H
H
Facultad de Química, Pontificia Universidad Católica, Santiago, Chile.
Cassels
B K
BK
Lissi
E A
EA
Videla
L A
LA
D000578:Amidines / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000737:chemistry*; D001060:Aporphines / Q000737:chemistry* / Q000494:pharmacology; D001923:Brain Chemistry; D004305:Dose-Response Relationship, Drug; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D015227:Lipid Peroxidation; D008163:Luminescent Measurements; D008297:Male; D009113:Muramidase / Q000037:antagonists & inhibitors; D010101:Oxygen Consumption; D051381:Rats; D011919:Rats, Inbred Strains
D000578:Amidines; D000975:Antioxidants; D001060:Aporphines; C046728:2,2'-azobis(2-amidinopropane); C011686:boldine; D009113:Muramidase