Alteration in L-type pyruvate kinase gene expression is not associated with the LF-B1 mRNA level.
The relation of expression of the LF-B1 gene with the L-type pyruvate kinase (L-PK) mRNA level in rat liver and hepatoma cells was investigated. The L-PK mRNA level in rat liver changed after partial hepatectomy, during development and on intake of a high carbohydrate diet, while the level of LF-B1 mRNA remained unchanged or altered reciprocally. Dedifferentiated AH-130 cells, which did not express L-PK mRNA, expressed LF-B1 mRNA. These results suggest that transcription of the pyruvate kinase L gene is not simply regulated by the level of LF-B1 mRNA.
Imai
E
E
Department of Nutrition and Physiological Chemistry, Osaka University Medical School, Japan.
Noguchi
T
T
Takenaka
M
M
Yamada
K
K
Matsuda
T
T
Monaci
P
P
Nicosia
A
A
Tanaka
T
T
D000818:Animals; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics; D004040:Dietary Carbohydrates / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic; D006498:Hepatectomy; D051537:Hepatocyte Nuclear Factor 1; D051538:Hepatocyte Nuclear Factor 1-alpha; D051539:Hepatocyte Nuclear Factor 1-beta; D007527:Isoenzymes / Q000235:genetics*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental; D008115:Liver Regeneration; D008297:Male; D009687:Nuclear Proteins; D011770:Pyruvate Kinase / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D004268:DNA-Binding Proteins; D004040:Dietary Carbohydrates; D051538:Hepatocyte Nuclear Factor 1-alpha; C496489:Hnf1a protein, rat; D007527:Isoenzymes; D009687:Nuclear Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D051537:Hepatocyte Nuclear Factor 1; D051539:Hepatocyte Nuclear Factor 1-beta; D011770:Pyruvate Kinase