Extended N-terminal sequencing of proteins of the large ribosomal subunit from yeast mitochondria.
We have determined the N-termini of 26 proteins of the large ribosomal subunit from yeast mitochondria by direct amino acid micro-sequencing. The N-terminal sequences of proteins YmL33 and YmL38 showed a significant similarity to eubacterial ribosomal (r-) proteins L30 and L14, respectively. In addition, several proteins could be assigned to their corresponding yeast nuclear genes. Based on a comparison of the protein sequences deduced from the corresponding DNA regions with the N-termini of the mature proteins, the putative leader peptides responsible for mitochondrial matrix-targeting were compiled. In most leader sequences a relative abundance of aromatic amino acids, preferentially phenylalanine, was found.
Grohmann
L
L
Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann, Berlin, Germany.
Graack
H R
HR
Kruft
V
V
Choli
T
T
Goldschmidt-Reisin
S
S
Kitakawa
M
M
D000595:Amino Acid Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000737:chemistry*; D008928:Mitochondria / Q000378:metabolism*; D008969:Molecular Sequence Data; D012269:Ribosomal Proteins / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D016415:Sequence Alignment
D005656:Fungal Proteins; D012269:Ribosomal Proteins