title: The assay of clozapine and N-desmethylclozapine in human plasma by high-performance liquid chromatography.
abstract: A high performance liquid chromatographic method for the simultaneous quantitation of clozapine--a member of the dibenzodiazepine class of antipsychotic drugs--and its reduced metabolite in human plasma has been developed. Clozapine and N-desmethylclozapine are concentrated from blood samples by liquid-liquid extraction, followed by reverse-phase liquid chromatography. Accuracy of the determination is ensured by application of an internal standard, protriptyline, which is closely related to clozapine. A detection limit of 15 ng/mL for clozapine and 30 ng/ml for N-desmethylclozapine was achieved.
authors.affiliation: Department of Psychiatry, College of Medicine, University of Iowa, Iowa City 52242.
authors.forename: M J
authors.initials: MJ
authors.lastname: Lovdahl
authors.forename: P J
authors.initials: PJ
authors.lastname: Perry
authors.forename: D D
authors.initials: DD
authors.lastname: Miller
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003024:Clozapine / Q000031:analogs & derivatives* / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results
chemical_list: C058272:norclozapine; D003024:Clozapine