In vivo microdialysis sampling for pharmacokinetic investigations.
In vivo microdialysis sampling coupled to liquid chromatography was used to study acetaminophen disposition in anesthetized rats. The pharmacokinetics of acetaminophen and its sulfate and glucuronide metabolites were determined using both microdialysis sampling and collection of whole blood. For microdialysis, samples were continuously collected for over 5 hr without fluid loss using a single experimental animal. Microdialysis sampling directly assesses the free drug concentration in blood. The pharmacokinetic results obtained with microdialysis sampling were the same as those obtained from blood collection. The administration of heparin, necessary when collecting blood samples, was found to double the elimination half-life of acetaminophen. Microdialysis sampling is a powerful tool for pharmacokinetic studies, providing accurate and precise pharmacokinetic data.
Scott
D O
DO
Department of Chemistry, University of Kansas, Lawrence 66045.
Sorenson
L R
LR
Steele
K L
KL
Puckett
D L
DL
Lunte
C E
CE
D000082:Acetaminophen / Q000031:analogs & derivatives / Q000097:blood / Q000493:pharmacokinetics*; D000818:Animals; D002853:Chromatography, Liquid; D003956:Dialysis / Q000379:methods; D004305:Dose-Response Relationship, Drug; D008832:Microchemistry / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D000082:Acetaminophen; C037386:acetaminophen glucuronide; C045667:acetaminophen sulfate ester