Development of an analytical method for cephapirin and its metabolite in bovine milk and serum by liquid chromatography with UV-VIS detection and confirmation by thermospray mass spectometry.
Metabolites of the cephapirin beta-lactam antibiotic have not previously been reported in bovine milk. The principal metabolite was tentatively identified as desacetylcephapirin by liquid chromatography with UV-VIS photodiode array (LC/UV-VIS PDA), and liquid-chromatography-mass-spectrometric (LC-MS) detection. Synthetic desacetylcephapirin was prepared by incubation of cephapirin in bovine milk and serum at 37 degrees C. Also, a method for determining cephapirin in bovine milk and serum was developed. The detection limits for cephapirin and desacetylcephapirin were estimated to be 10 and 50 micrograms/kg, respectively, for LC/UV-VIS PDA, and 100 and 500 micrograms/kg for LC-MS.
Tyczkowska
K L
KL
Department of Anatomy, Physiological Sciences, and Radiology, College of Veterinary Medicine, North Carolina State University, Raleigh.
Voyksner
R D
RD
Aronson
A L
AL
D000818:Animals; D002417:Cattle; D002514:Cephapirin / Q000032:analysis* / Q000097:blood; D002853:Chromatography, Liquid / Q000379:methods / Q000662:veterinary; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods / Q000662:veterinary; D008892:Milk / Q000737:chemistry*
D002514:Cephapirin