The effects of iris-ciliary complex on the organ cultured rabbit ocular lens.
Freshly isolated rabbit lenses were cultured with and without attached iris-ciliary (IC)-complex for 24 hours in TC-199 medium. Subsequent morphological analysis revealed that the IC-complex cannot be maintained in serum-free medium. In addition an observed effect of the IC-complex on the co-cultured lenses could not be due only because of the cellular degeneration of the IC-complex. To test this possibility lenses with attached IC-complexes were incubated in 20% serum-containing TC-199 medium. The IC-complex cultured in 20% serum containing medium retained its normal morphology. However co-cultured lens cells displayed vacuoles and other signs of degeneration. The protein synthetic and Na+/K+ pump activities of these lenses were also significantly depressed. These data indicated that the observed effects of IC-complex on the lens were not due to its cellular degradation. Preliminary experiments showed that the IC-complex contains water soluble factor(s) which could effectively inhibit lens protein synthesis and Na+/K+ pump.
Niyogi
T K
TK
Department of Anatomy and Cell Biology, Wayne State University, School of Medicine, Detroit.
Emanuel
K
K
Parafina
J
J
Bagchi
M
M
D000818:Animals; D001345:Autoradiography; D002470:Cell Survival; D002924:Ciliary Body / Q000166:cytology*; D003459:Crystallins / Q000378:metabolism; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D007498:Iris / Q000166:cytology*; D007908:Lens, Crystalline / Q000378:metabolism* / Q000473:pathology; D009924:Organ Culture Techniques; D011188:Potassium / Q000378:metabolism; D011817:Rabbits; D012964:Sodium / Q000378:metabolism
D003459:Crystallins; D003470:Culture Media; D012964:Sodium; D011188:Potassium