Species variation in organellar location and activity of L-pipecolic acid oxidation in mammals.
The oxidation of L-pipecolic acid to alpha-aminoadipic acid was studied in eight species of mammals using an assay system more sensitive than those previously employed. After percoll-gradient fractionation, activity was localized to the mitochondrial-enriched fractions in tissues from rabbit, guinea pig, pig, dog, and sheep, with guinea pig kidney cortex showing greatest specific activity. These results contrast with the peroxisomal oxidation of L-pipecolic acid observed in macaques and man (Mihalik and Rhead 1989; Mihalik et al. 1989). Rats and mice had undetectable levels of both peroxisomal and mitochondrial L-pipecolic acid oxidation. In the rat, peroxisomal oxidation activity was not induced by feeding with either clofibrate or clofibrate and L-pipecolic acid. Thus, among mammals, both the ability to oxidize L-pipecolic acid and the organellar location of this oxidation is species dependent.
Mihalik
S J
SJ
Department of Pediatrics, University of Iowa, Iowa City 52242.
Rhead
W J
WJ
D000818:Animals; D004285:Dogs; D006168:Guinea Pigs; D007672:Kidney Cortex / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008322:Mammals / Q000378:metabolism*; D051379:Mice; D008830:Microbodies / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D015388:Organelles / Q000378:metabolism; D010084:Oxidation-Reduction; D010875:Pipecolic Acids / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D012756:Sheep; D013045:Species Specificity; D013552:Swine
D010875:Pipecolic Acids; C031345:pipecolic acid