title: A putative RNA virus in Babesia bovis.
abstract: Babesia bovis is an intraerythrocytic protozoan that causes bovine babesiosis. Agarose gel electrophoresis of nucleic acids extracted from two isolates of B. bovis reveals, besides bulk DNA, an ethidium bromide-stainable band at about 5.5 kb. Further characterization of the latter with DNase I, RNase and mung bean nuclease suggested it to be a double-stranded RNA. Sonicated parasites were fractionated in a CsCl buoyant density gradient. A sample containing the 5.5-kb RNA was analysed under an electron microscope and a virus-like particle was observed.
authors.affiliation: Department of Biotechnology, Universidade Federal do Rio Grande do Sul, Porto Alegre-RS, Brazil.
authors.forename: R C
authors.initials: RC
authors.lastname: Johnston
authors.forename: N A
authors.initials: NA
authors.lastname: Farias
authors.forename: J C
authors.initials: JC
authors.lastname: Gonzales
authors.forename: H
authors.initials: H
authors.lastname: Dewes
authors.forename: A
authors.initials: A
authors.lastname: Masuda
authors.forename: C
authors.initials: C
authors.lastname: Termignoni
authors.forename: K
authors.initials: K
authors.lastname: Amako
authors.forename: L S
authors.initials: LS
authors.lastname: Ozaki
mesh_terms: D000818:Animals; D001403:Babesia / Q000235:genetics / Q000382:microbiology*; D001404:Babesiosis / Q000235:genetics; D002417:Cattle; D002499:Centrifugation, Density Gradient; D003850:Deoxyribonuclease I; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*; D012259:Ribonuclease, Pancreatic
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral; D003850:Deoxyribonuclease I; D012259:Ribonuclease, Pancreatic