title: Micro method for liquid chromatographic determination of cyclosporin A in whole blood with use of a rapid extraction procedure.
abstract: In this precise, reversed-phase liquid chromatographic procedure, Cyclosporin A (Cy A) is extracted from 300 microL of whole blood with cyclosporin D as internal standard by liquid-liquid extraction. A 20-microL aliquot of the extract, injected onto a Nucleosil octyl analytical column heated at 72 degrees C, is eluted with a mixture of acetonitrile and 0.01 M phosphate buffer, pH 5.5 at a flow rate of 1 mL/min. Detection is set at 210 nm. The chromatography is complete within 10 min. The detection limit is 25 micrograms/L. Between-run CVs range from 2.3 to 6.2% and recovery is 92.1 +/- 6.3%. The major advantages of our extraction procedure are the rapid clean-up method and the small volume required. This procedure is especially suitable for cyclosporine determination in pediatric transplantations.
authors.affiliation: Laboratoire de Toxicologie, Faculté de Pharmacie, Paris, France.
authors.forename: N
authors.initials: N
authors.lastname: Sadeg
authors.forename: P H
authors.initials: PH
authors.lastname: Chuong
authors.forename: J R
authors.initials: JR
authors.lastname: Claude
authors.forename: M
authors.initials: M
authors.lastname: Hamon
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003524:Cyclosporins / Q000032:analysis / Q000097:blood*; D006801:Humans; D013056:Spectrophotometry, Ultraviolet
chemical_list: D003524:Cyclosporins; C050026:cyclosporin D