Chromosome aberration dosimetry: addition of thymidine, an interesting mean to increase the number of dividing cells.
Addition of thymidine to culture medium commonly used by laboratories performing medical cytogenetics followed by a release of the block by 2-deoxycitidine increases greatly the mitotic index and the proportion of metaphases suitable for chromosome analysis. The benefit is, however, less evident when the method is applied to culture medium used in laboratories specialized in chromosome aberration dosimetry.
Dutrillaux
B
B
Institut Curie, Paris, France.
Hilali
A
A
Achkar
W A
WA
Martins
B
B
Sabatier
L
L
Léonard
E D
ED
Léonard
A
A
D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D002869:Chromosome Aberrations; D003078:Colchicine / Q000494:pharmacology; D003470:Culture Media; D003841:Deoxycytidine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006801:Humans; D008940:Mitotic Index / Q000187:drug effects / Q000528:radiation effects; D013936:Thymidine / Q000494:pharmacology*
D003470:Culture Media; D003841:Deoxycytidine; D003078:Colchicine; D013936:Thymidine