A simple method for the recovery of purified recombinant peptides cleaved from glutathione-S-transferase-fusion proteins.
Abath
F G
FG
National Institute for Medical Research, Division of Parasitology, London, United Kingdom.
Simpson
A J
AJ
D000818:Animals; D002498:Centrifugation; D003001:Cloning, Molecular / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism*; D015801:Helminth Proteins / Q000235:genetics / Q000302:isolation & purification; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000302:isolation & purification*; D012550:Schistosoma mansoni / Q000235:genetics; D013917:Thrombin / Q000378:metabolism; D013997:Time Factors
D015801:Helminth Proteins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D005982:Glutathione Transferase; D013917:Thrombin