Direct evidence for the involvement of free radicals in ischemic insult to the intestine.
Ischemia of rat intestine was induced in vivo by occlusion of the superior mesenteric artery (SMA) for 15 min. Sodium salicylate, 100 mg/kg, given IP, 30 min prior to the ischemic event served as a specific trap for hydroxyl radicals and provided direct evidence for the involvement of free radicals during the ischemic insult. Portions of the bowel were sequentially isolated and removed. The hydroxylation products, dihydroxybenzoic acid (DHBA) derivatives were isolated, identified and quantified by HPLC coupled with electrochemical detection (ECD). The level of 2,5-DHBA (Mean +/- SE, ng/g tissue) in the preischemic bowel (N = 21) was 241.8 +/- 10.0. It rose significantly to 313.3 +/- 15.5 in the ischemic specimen (p = 0.0129) and remained unchanged in the reperfusion period (322.8 +/- 15.5). The histological examination correlated well with these levels: mild villi damage in the ischemic period with no further damage in the reperfusion period.
Udassin
R
R
Department of Pediatric Surgery, Hadassah University Hospital, Jerusalem, Israel.
Arieli
I
I
Haskel
Y
Y
Kitrossky
N
N
Chevion
M
M
D000818:Animals; D004195:Disease Models, Animal; D005609:Free Radicals; D005841:Gentisates; D062385:Hydroxybenzoates / Q000378:metabolism; D007422:Intestines / Q000098:blood supply*; D008297:Male; D051381:Rats; D015427:Reperfusion Injury / Q000378:metabolism*
D005609:Free Radicals; D005841:Gentisates; D062385:Hydroxybenzoates; C010925:2,5-dihydroxybenzoic acid