Potential interference of hydrogen peroxide in the 2,2'-bicinchoninic acid protein assay.
At a concentration of 20-800 nmol/0.1 ml hydrogen peroxide instantly reacts with a 2,2'-bicinchoninic acid copper color reagent. It also reacts with a reformulated reagent at pH 7 but the color develops less rapidly. While the effect may interfere with protein estimations at alkaline pH, the effect of pH 7 may be used to determine the possible extent of hydrogen peroxide interference after treatment with catalase.
Baker
W L
WL
Chemistry Department, Swinburne Institute of Technology, Hawthorn, Victoria, Australia.
D002374:Catalase; D003124:Colorimetry; D006861:Hydrogen Peroxide; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D011506:Proteins / Q000737:chemistry*; D011804:Quinolines
D007202:Indicators and Reagents; D011506:Proteins; D011804:Quinolines; D006861:Hydrogen Peroxide; C047117:bicinchoninic acid; D002374:Catalase