Isolation of murine sialoglycoprotein using consecutive chromatography.
Affinity columns and high performance liquid chromatography were employed consecutively to obtain 89, 65, 46 and 29 kilodalton sialoglycoproteins from mouse erythrocyte ghosts free of the Band 3 protein which traditionally co-purifies with these proteins. The purification scheme involves Concanavalin A, Wheat Germ Agglutinin and/or Limulus lectin Sepharose 4B columns. We have designated these glycophorin-like proteins Sialoglycoproteins 1, 2, 3, and 4, respectively. Sialoglycoprotein 2 can be isolated independently using a Limulus column combination, while Sialoglycoproteins 3 and 4 were isolated separately during high performance liquid chromatography, demonstrating heterogeneity in binding properties between these sialoglycoproteins.
Wilson
D J
DJ
Division of Biomedical Sciences, School of Graduate Studies, Meharry Medical College, Nashville, Tennessee 37208.
Planas
J M
JM
D000818:Animals; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D003208:Concanavalin A / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000737:chemistry*; D006737:Horseshoe Crabs; D037102:Lectins / Q000737:chemistry; D051379:Mice; D012795:Sialoglycoproteins / Q000302:isolation & purification*; D014909:Wheat Germ Agglutinins / Q000737:chemistry
D037102:Lectins; D012795:Sialoglycoproteins; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A