title: LAV revisited: origins of the early HIV-1 isolates from Institut Pasteur.
abstract: Two of the first human immunodeficiency virus type-1 (HIV-1) strains isolated were authenticated by reanalyzing original cultured samples stored at the Collection Nationale de Culture des Microorganismes as well as uncultured primary material. Cloned polymerase chain reaction products were used to analyze coding sequences of the V3 loop in the gp120 glycoprotein. The original isolate HIV-1 Bru, formerly called LAV, was derived from patient BRU. HIV-1 Lai was derived from patient LAI and contaminated a HIV-1 Bru culture between 20 July and 3 August 1983. The culture became, in effect, HIV-1 Lai, identifiable by a unique motif in the V3 loop. Because of this contamination two, rather than one, HIV-1 isolates were sent to the Laboratory of Tumor Cell Biology at the National Cancer Institute on 23 September 1983. Original HIV-1 Bru was indeed present in the sample marked JBB/LAV. However the M2T-/B sample harbored HIV-1 Lai, a strain capable of growing on established cell lines. The striking similarity between HIV-1 Lai (formerly LAV-Bru) and HTLV-3B sequences remains.
authors.affiliation: Laboratoire de Rétrovirologie Moléculaire, Institut Pasteur, Paris.
authors.forename: S
authors.initials: S
authors.lastname: Wain-Hobson
authors.forename: J P
authors.initials: JP
authors.lastname: Vartanian
authors.forename: M
authors.initials: M
authors.lastname: Henry
authors.forename: N
authors.initials: N
authors.lastname: Chenciner
authors.forename: R
authors.initials: R
authors.lastname: Cheynier
authors.forename: S
authors.initials: S
authors.lastname: Delassus
authors.forename: L P
authors.initials: LP
authors.lastname: Martins
authors.forename: M
authors.initials: M
authors.lastname: Sala
authors.forename: M T
authors.initials: MT
authors.lastname: Nugeyre
authors.forename: D
authors.initials: D
authors.lastname: Guétard
mesh_terms: D000047:Academies and Institutes; D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology* / Q000473:pathology; D000595:Amino Acid Sequence; D001483:Base Sequence; D001706:Biopsy; D002460:Cell Line; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005602:France; D015699:HIV Envelope Protein gp120 / Q000235:genetics*; D015497:HIV-1 / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D008198:Lymph Nodes / Q000382:microbiology / Q000473:pathology; D008297:Male; D008969:Molecular Sequence Data; D009316:National Institutes of Health (U.S.); D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods; D012689:Sequence Homology, Nucleic Acid; D014481:United States; D014773:Virology / Q000379:methods
chemical_list: D004279:DNA, Viral; D015699:HIV Envelope Protein gp120; D015345:Oligonucleotide Probes