Hamster cheek-pouch testing of dental soft polymers.
The hamster cheek pouch provides a suitable model system for the mucous-membrane irritation testing of dental materials. Poor retention of materials or difficulties in histopathological interpretation caused by surgical artifacts have been reported in published techniques. We describe a new "pouch-in-pouch" technique for mucous membrane irritation tests. The retention rate of polymer discs was 97% and 87% at 14 and 35 days, respectively. Clear differentiation was obtained between the tissue reaction to the test materials and the surgical procedure. Polymer discs containing dibutyltin diacetate (DBTD) or dibutyl phthalate (DBP) as plasticizer resulted in epithelial changes, including epithelial atypia, early papillomas, and areas resembling dysplasia. The potentially pre-malignant nature of these changes requires further investigation.
Harsanyi
B B
BB
Division of Oral Pathology, Faculty of Dentistry, Dalhousie University, Halifax, Nova Scotia, Canada.
Foong
W C
WC
Howell
R E
RE
Hidi
P
P
Jones
D W
DW
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D006224:Cricetinae; D003764:Dental Materials / Q000737:chemistry* / Q000494:pharmacology; D003822:Denture Liners; D003993:Dibutyl Phthalate / Q000737:chemistry* / Q000494:pharmacology; D004848:Epithelium / Q000473:pathology; D006965:Hyperplasia; D008422:Materials Testing / Q000379:methods*; D008647:Mesocricetus; D009061:Mouth Mucosa / Q000187:drug effects / Q000473:pathology*; D009947:Organotin Compounds / Q000737:chemistry* / Q000494:pharmacology; D010968:Plasticizers / Q000737:chemistry* / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D013280:Stomatitis / Q000473:pathology
D001672:Biocompatible Materials; D003764:Dental Materials; D009947:Organotin Compounds; D010968:Plasticizers; D011108:Polymers; D003993:Dibutyl Phthalate; C004924:dibutyltin diacetate