Flush perfusion of rabbit kidneys with autogeneic, allogeneic and xenogeneic blood.
A simple perfusion model was developed to study the events that lead to rejection of renal xenografts. Flush perfusion of the kidneys of 24 rabbits was carried out with blood from rabbits, cats, or humans. Light microscopy showed alterations with neutrophilic granulocytes margination of the vessels after only 15 min of perfusion. In the experiments with human blood the immunofluorescence microscopy showed deposits of immunoglobulins and complement factor III. This simple method is therefore a useful way of studying perfusion in renal xenografts.
Kemp
E
E
Department of Nephrology, Odense University Hospital, Denmark.
Larsen
S
S
Jørgensen
K A
KA
Dieperink
H
H
Starklint
H
H
D000818:Animals; D001769:Blood / Q000276:immunology; D002415:Cats; D003176:Complement C3 / Q000032:analysis; D004730:Endothelium, Vascular / Q000276:immunology; D005340:Fibrinogen / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006084:Graft Rejection / Q000276:immunology*; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D007678:Kidney Glomerulus / Q000276:immunology; D016030:Kidney Transplantation / Q000276:immunology*; D010477:Perfusion / Q000295:instrumentation*; D010974:Platelet Aggregation / Q000276:immunology; D011817:Rabbits; D014183:Transplantation, Heterologous / Q000276:immunology
D003176:Complement C3; D007136:Immunoglobulins; D005340:Fibrinogen