A simplified method for screening the apolipoprotein E polymorphism.
A simple method for screening populations for the apolipoprotein E polymorphism which involves isoelectric focusing of delipidated samples on polyacrylamide gels of pH 4.5-5.8 followed by immunoblotting using a double-antibody technique is presented. The method is a synthesis of two previously published procedures and works well on samples that have been stored for as long as 15 years.
Eichner
J E
JE
Department of Epidemiology, Graduate School of Public Health, University of Pittsburgh, Pa.
Kuller
L H
LH
Ferrell
R E
RE
Kamboh
M I
MI
D000328:Adult; D001057:Apolipoproteins E / Q000235:genetics*; D002318:Cardiovascular Diseases / Q000175:diagnosis / Q000235:genetics; D005260:Female; D005820:Genetic Testing; D006801:Humans; D007525:Isoelectric Focusing; D008137:Longitudinal Studies; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012307:Risk Factors
D001057:Apolipoproteins E