Gasoline pump mechanics had increased frequencies and sizes of micronuclei in lymphocytes stimulated by pokeweed mitogen.
Fifteen gasoline pump mechanics and 15 controls were investigated with the lymphocyte micronucleus assay and also with differential count of leukocytes in the peripheral blood. The lymphocytes were stimulated with phytohemagglutinin and pokeweed mitogen in parallel cultures. The pump mechanics had increased frequencies and sizes of micronuclei in the pokeweed mitogen-stimulated cultures but not in those stimulated with phytohemagglutinin. The difference in effect between the 2 mitogens is probably due to an increased sensitivity of the B-lymphocytes to mutagens. The leukocyte count was significantly higher in the gasoline-exposed group. The effect of the occupational exposure was probably caused by the benzene content of the gasoline. The time-weighted average for 8 h was around 1 mg/m3 of benzene, but there were peak levels of 20 mg/m3.
Högstedt
B
B
Department of Occupational Medicine, Central Hospital, Halmstad, Sweden.
Holmén
A
A
Karlsson
A
A
Raihle
G
G
Nillius
K
K
Vestlund
K
K
D000328:Adult; D002869:Chromosome Aberrations; D005742:Gasoline / Q000009:adverse effects*; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000648:ultrastructure*; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000648:ultrastructure*; D015162:Micronucleus Tests; D008875:Middle Aged; D016273:Occupational Exposure; D010835:Phytohemagglutinins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology*
D005742:Gasoline; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens