Resolution of 4-chlorobenzoate dehalogenase from Pseudomonas sp. strain CBS3 into three components.
Extracts of Pseudomonas sp. strain CBS3 grown with 4-chlorobenzoate as sole carbon source contained an enzyme that converted 4-chlorobenzoate to 4-hydroxybenzoate. This enzyme was shown to consist of three components, all necessary for the reaction. Component I, which had a molecular weight of about 3,000, was highly unstable. Components II and III were stable proteins with molecular weights of about 86,000 and 92,000.
Elsner
A
A
Institut für Mikrobiologie, Universität Hohenheim, Stuttgart, Federal Republic of Germany.
Löffler
F
F
Miyashita
K
K
Müller
R
R
Lingens
F
F
D006867:Hydrolases / Q000737:chemistry / Q000302:isolation & purification*; D008970:Molecular Weight; D011549:Pseudomonas / Q000201:enzymology*
D006867:Hydrolases; C042846:4-chlorobenzoate dehalogenase