title: Production of monoclonal antibodies against a hemagglutinin/protease of Vibrio cholerae non-01.
abstract: Two hybridoma cell lines producing monoclonal antibodies (MAbs) against a hemagglutinin/protease (HA/P) from Vibrio cholerae non-01 were produced and characterized. The two MAbs contained the kappa light chain and were IgG1 type. They similarly neutralized HA/P protease activity derived from both V. cholerae non-01 and V. cholerae 01, whereas they were unable to neutralize the hemagglutinating activity of HA/P, suggesting that the epitopes for protease and hemagglutination activities are different. Western blotting analysis and the cross-neutralization test with the two MAbs confirmed the identity of HA/P produced by V. cholerae non-01 and 01. This study also suggests that HA/P of V. cholerae and a protease of V. parahaemolyticus are immunologically unrelated.
authors.affiliation: Research Institute for Microbial Diseases, Osaka University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Honda
authors.forename: A
authors.initials: A
authors.lastname: Hata-Naka
authors.forename: K
authors.initials: K
authors.lastname: Lertpocasombat
authors.forename: T
authors.initials: T
authors.lastname: Miwatani
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D006384:Hemagglutination; D006825:Hybridomas; D008666:Metalloendopeptidases / Q000276:immunology*; D051379:Mice; D009500:Neutralization Tests; D014734:Vibrio cholerae / Q000201:enzymology / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D008666:Metalloendopeptidases; C039814:hemagglutinin-protease