Projections from the rostral ventrolateral medulla to brainstem monoamine neurons in the rat.
Following the iontophoretic deposition of Phaseolus vulgaris leucoagglutinin (PHA-L) into the rostral ventrolateral medulla (RVL), two-color immunoperoxidase staining was employed to demonstrate contiguity between PHA-L-immunoreactive (PHA-LI) varicose fibers and boutons and brainstem monoaminergic cells. Black-stained PHA-LI cells in the deposition site were found to be located among amber-stained phenylethanolamine N-methyl transferase-immunoreactive (PNMT-I) neurons of the C1 cell group. RVL projections were contiguous with PNMT-I neurons of the C1, C2 and C3 cell groups, with tyrosine hydroxylase-immunoreactive (TH-I) neurons of the A1, A2 and A5 cell groups, and with serotonin-immunoreactive (5-HT-I) neurons of the B1, B2 and B3 cell groups. Preliminary findings of this study have been presented previously (Soc. Neurosci. Abstr., 15 (1989) 451).
Nicholas
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AP
Department of Anatomy and Neurosciences, University of Texas Medical Branch, Galveston 77550.
Hancock
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MB
D000818:Animals; D015306:Biogenic Monoamines / Q000502:physiology*; D001933:Brain Stem / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D007478:Iontophoresis; D008297:Male; D008526:Medulla Oblongata / Q000166:cytology* / Q000201:enzymology / Q000502:physiology; D009474:Neurons / Q000201:enzymology / Q000502:physiology*; D010625:Phenylethanolamine N-Methyltransferase / Q000378:metabolism; D010835:Phytohemagglutinins; D011149:Pons / Q000166:cytology; D051381:Rats; D011919:Rats, Inbred Strains
D015306:Biogenic Monoamines; D010835:Phytohemagglutinins; C100156:leukoagglutinins, plants; D010625:Phenylethanolamine N-Methyltransferase