title: Immunocytochemical localization of cathepsins B, H, and L in the rat gastro-duodenal mucosa.
abstract: Cathepsins B, H, and L are representative cysteine proteinases in lysosomes of a large variety of cells. Previous immunochemical studies indicated the presence of these enzymes also in the gastrointestinal wall. Using specific antisera, the cellular and subcellular distribution of cathepsins B, H, and L in rat gastric (oxyntic and pyloric part) and duodenal mucosa was investigated by light and electron microscopical immunocytochemistry. The subtypes of cathepsins were distributed differently in the cellular constituents of the epithelia: Cathepsin B was localized to lysosomes of all cells except goblet cells. Cathepsin H was found predominantly in gastric parietal cells (lysosomes) and in secretion granules of pyloric gastrin and duodenal cholecystokinin cells. Cathepsin L immunoreactivities were weak and restricted to a minority of cells (gastric mucous cells, enterocytes). Interstitial cells of the lamina propria immunoreactive for cathepsins H and L were identified as macrophages. The present findings suggest a dual function of cathepsins in the gastro-duodenal mucosa. They (1) cleave enzymatically proteins and peptides ingested in lysosomes, and (2) they may be involved in the processing of biologically active peptides (enteric hormones) from their precursor proteins.
authors.affiliation: Department of Gastrointestinal Internal Medicine, University of Tsukuba, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Furuhashi
authors.forename: A
authors.initials: A
authors.lastname: Nakahara
authors.forename: H
authors.initials: H
authors.lastname: Fukutomi
authors.forename: E
authors.initials: E
authors.lastname: Kominami
authors.forename: D
authors.initials: D
authors.lastname: Grube
authors.forename: Y
authors.initials: Y
authors.lastname: Uchiyama
mesh_terms: D000818:Animals; D002401:Cathepsin B / Q000032:analysis; D056655:Cathepsin H; D056668:Cathepsin L; D002403:Cathepsins / Q000032:analysis*; D003546:Cysteine Endopeptidases; D004386:Duodenum / Q000201:enzymology; D010450:Endopeptidases; D005753:Gastric Mucosa / Q000201:enzymology*; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000201:enzymology*; D008297:Male; D016253:Microscopy, Immunoelectron; D011708:Pylorus; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D002401:Cathepsin B; D056668:Cathepsin L; C534280:Ctsl protein, rat; D056655:Cathepsin H; C534128:Ctsh protein, rat