title: Characterization of a murine gene expressed from the inactive X chromosome.
abstract: In mammals, equal dosage of gene products encoded by the X chromosome in male and female cells is achieved by X inactivation. Although X-chromosome inactivation represents the most extensive example known of long range cis gene regulation, the mechanism by which thousands of genes on only one of a pair of identical chromosomes are turned off is poorly understood. We have recently identified a human gene (XIST) exclusively expressed from the inactive X chromosome. Here we report the isolation and characterization of its murine homologue (Xist) which localizes to the mouse X inactivation centre region and is the first murine gene found to be expressed from the inactive X chromosome. Nucleotide sequence analysis indicates that Xist may be associated with a protein product. The similar map positions and expression patterns for Xist in mouse and man suggest that this gene may have a role in X inactivation.
authors.affiliation: Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.
authors.forename: G
authors.initials: G
authors.lastname: Borsani
authors.forename: R
authors.initials: R
authors.lastname: Tonlorenzi
authors.forename: M C
authors.initials: MC
authors.lastname: Simmler
authors.forename: L
authors.initials: L
authors.lastname: Dandolo
authors.forename: D
authors.initials: D
authors.lastname: Arnaud
authors.forename: V
authors.initials: V
authors.lastname: Capra
authors.forename: M
authors.initials: M
authors.lastname: Grompe
authors.forename: A
authors.initials: A
authors.lastname: Pizzuti
authors.forename: D
authors.initials: D
authors.lastname: Muzny
authors.forename: C
authors.initials: C
authors.lastname: Lawrence
authors.forename: H F
authors.initials: HF
authors.lastname: Willard
authors.forename: P
authors.initials: P
authors.lastname: Avner
authors.forename: A
authors.initials: A
authors.lastname: Ballabio
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003360:Cosmids; D003433:Crosses, Genetic; D004247:DNA / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008540:Meiosis; D051379:Mice; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods; D011995:Recombination, Genetic; D012689:Sequence Homology, Nucleic Acid; D014960:X Chromosome
chemical_list: D015345:Oligonucleotide Probes; D004247:DNA