Analysis of the antigenic difference between Vero toxin 2 (VT2) and VT2 variant (VT2vh) of Verotoxin-producing Escherichia coli by a site-directed mutagenesis.
Ouchterlony double gel diffusion analysis of the A and B subunits of purified Vero toxin 2 (VT2) and a variant of VT2 (VT2vh) demonstrated that the difference in antigenicity between VT2 and VT2vh is due to the difference in the B subunit of the two toxins. Analysis of mutants of VT2vh prepared by site-directed mutagenesis attributed the antigenic dissimilarity to the difference in the amino acid residue at position 24 of the B subunit.
Ito
H
H
Department of Microbiology, Faculty of Medicine, Kyoto University, Japan.
Nishibuchi
M
M
Takeda
Y
Y
D000940:Antigenic Variation; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D022622:Shiga Toxin 1
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D022622:Shiga Toxin 1