title: Simple high-performance liquid chromatographic method for the determination of PGT/1A, a new immunostimulating drug, in biological fluids.
abstract: This paper describes a simple high-performance liquid chromatographic method for the determination of PGT/1A (3-L-pyroglutamyl-L-thiazolidine-4-carboxylic acid), a new immunostimulating drug, in plasma and urine. The column was packed with LiChrospher-NH2 (5 microns), the mobile phase was 0.02 M monobasic potassium phosphate (pH 3.2 with concentrated phosphoric acid)-acetonitrile (25:75, v/v), the flow-rate was 1.2 ml/min, the detection wavelength was 210 nm and the apparatus was a Varian Model 5000. Plasma (1 ml) was added to 1.2 ml of acetonitrile and the supernatant injected; the urine was diluted 1:5. The retention time of PGT/1A was 9.4 min in plasma and 9.9 min in urine. The method was validated for recovery, accuracy and reproducibility. The results after intravenous injection in twelve volunteers are also given.
authors.affiliation: Poli Research Center, Milan, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Coppi
authors.forename: M
authors.initials: M
authors.lastname: Barchielli
mesh_terms: D000097:Acetonitriles / Q000008:administration & dosage; D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000097:blood* / Q000652:urine; D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007275:Injections, Intravenous; D008297:Male; D010710:Phosphates / Q000008:administration & dosage; D011188:Potassium / Q000008:administration & dosage; D017680:Potassium Compounds; D011761:Pyrrolidonecarboxylic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D013844:Thiazoles / Q000008:administration & dosage / Q000097:blood* / Q000652:urine; D053778:Thiazolidines
chemical_list: D000097:Acetonitriles; D000276:Adjuvants, Immunologic; D010710:Phosphates; D017680:Potassium Compounds; D013844:Thiazoles; D053778:Thiazolidines; C064893:pidotimod; C013216:potassium phosphate; D011188:Potassium; D011761:Pyrrolidonecarboxylic Acid; C032159:acetonitrile