Incorporation of exogenous uracil by Cryptosporidium parvum in vitro.
Oocysts of Cryptosporidium parvum were used to infect Madin-Darby bovine kidney cells. Cultures were incubated in a reduced-oxygen atmosphere in candle jars or in a 5% CO2-95% air atmosphere. At 72 h, parasites were quantitated microscopically and found to be enhanced 5.5-fold in the reduced-oxygen atmosphere. Using candle jars, we then determined that C. parvum was amenable to [3H]uracil incorporation assays and easily quantitated with this method.
Upton
S J
SJ
Division of Biology, Kansas State University, Manhattan 66506.
Tilley
M
M
Mitschler
R R
RR
Oppert
B S
BS
D000818:Animals; D002460:Cell Line; D003458:Cryptosporidium / Q000254:growth & development / Q000378:metabolism*; D014498:Uracil / Q000378:metabolism*
D014498:Uracil