title: NCAM polysialic acid can regulate both cell-cell and cell-substrate interactions.
abstract: We have proposed previously that the polysialic acid (PSA) moiety of NCAM can influence membrane-membrane apposition, and thereby serve as a selective regulator of a variety of contact-dependent cell interactions. In this study, cell and tissue culture models are used to obtain direct evidence that the presence of PSA on the surface membrane can affect both cell-cell and cell-substrate interactions. Using a neuroblastoma/sensory neuron cell hybrid, it was found that removal of PSA with a specific neuraminidase (endo-N) augments cell-cell aggregation mediated by the L1 cell adhesion molecule as well as cell attachment to a variety of tissue culture substrates. In studies of embryonic spinal cord axon bundling, which involves both cell-cell and cell-substrate interactions, the pronounced defasciculation produced by removal of PSA is most easily explained by an increase in cell-substrate interaction. The fact that in both studies NCAM's intrinsic adhesion function was found not to be an important variable further illustrates that regulation of the cell surface by PSA can extend beyond binding mediated by the NCAM polypeptide.
authors.affiliation: Department of Anatomy and Cell Biology, University of Alberta, Edmonton, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Acheson
authors.forename: J L
authors.initials: JL
authors.lastname: Sunshine
authors.forename: U
authors.initials: U
authors.lastname: Rutishauser
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D002448:Cell Adhesion; D015816:Cell Adhesion Molecules, Neuronal / Q000502:physiology*; D002449:Cell Aggregation; D002642:Chick Embryo; D046508:Culture Techniques; D006822:Hybrid Cells; D007797:Laminin / Q000502:physiology; D008954:Models, Biological; D009439:Neuraminidase / Q000378:metabolism; D011134:Polysaccharides / Q000502:physiology*; D012794:Sialic Acids / Q000502:physiology*; D013116:Spinal Cord / Q000737:chemistry
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D007797:Laminin; D011134:Polysaccharides; D012794:Sialic Acids; C021319:polysialic acid; D009439:Neuraminidase