Oversensitivity to serotonin of the collateralized vascular bed in rat hindquarters: mechanisms of increased vasoconstriction.
A buffered solution was perfused at a constant flow rate (2 ml/min) through both iliac arteries in rat hindquarters. Perfusion pressures were measured in normal and collateralized vascular beds of the left and right hind-leg, respectively. Bolus injections of various agonists produced concentration-dependent increases in perfusion pressure in both collateralized and normal circulatory beds. Serotonin, in particular, and noradrenaline, to a lesser extent, produced more pronounced vasoconstriction on the collateral side than on the normal side. The difference in vasoreactivity to serotonin was related to a difference in both vascular structure and sensitivity of both types of vascular bed. Vasoconstriction induced by serotonin was inhibited by 5-HT2 antagonists. Selective blockade of alpha 1,alpha 2,beta 1-beta 2 adrenoceptors and amine uptake blockade were ineffective. This study indicates that, in rat hind-legs, the collateralized vascular bed is superreactive to serotonin in comparison with the normal bed. This resetting of reactivity to serotonin is due to the specific vascular structure as well as to an increased 5-HT2 receptor-mediated sensitivity.
Verheyen
A
A
Department of Cardiovascular Pharmacology, Medicinal Chemistry and Pharmacology, Janssen Research Foundation, Beerse, Belgium.
Lauwers
F
F
Vlaminckx
E
E
Wouters
L
L
de Clerck
F
F
D000818:Animals; D003097:Collateral Circulation / Q000187:drug effects* / Q000502:physiology; D006614:Hindlimb / Q000098:blood supply; D008297:Male; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000494:pharmacology*; D012702:Serotonin Antagonists; D014661:Vasoconstriction / Q000187:drug effects* / Q000502:physiology
D012702:Serotonin Antagonists; D012701:Serotonin