HIV-1 infection and expression in human colonic cells: infection and expression in CD4+ and CD4- cell lines.
Three human colonic epithelial cell lines, SW620, HT29, and T84, were characterized with respect to HIV-1 infection and gene expression. SW620 and HT29, but not T84, could be infected with HIV-1. CD4 messenger RNA and its protein product were identified in SW620 cells but not in HT29 or T84 cells. Anti-CD4 antibody blocked infection of SW620 cells but had no effect on infection of HT29 cells. In SW620 and HT29 cells transfected with the HIV-1 long terminal repeat (LTR) linked to the chloramphenicol acetyl transferase (CAT) reporter gene, an intact HIV-1 enhancer element was required for stimulation of CAT activity by tumor necrosis factor alpha (TNF alpha) and phorbol ester. T84 was not able to mediate a TNF alpha or phorbol ester response. These studies provide further evidence that HIV-1 can infect cells by mechanisms other than those mediated by the CD4 receptor and describe complementary models for analyzing HIV-1 infection and expression in colonic epithelial cells.
Omary
M B
MB
Department of Medicine, University of California, San Diego, La Jolla 92093-0623.
Brenner
D A
DA
de Grandpre
L Y
LY
Roebuck
K A
KA
Richman
D D
DD
Kagnoff
M F
MF
D015704:CD4 Antigens / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D003106:Colon / Q000166:cytology*; D004847:Epithelial Cells; D004848:Epithelium / Q000382:microbiology; D015967:Gene Expression Regulation, Viral; D016322:HIV Enhancer / Q000187:drug effects; D016325:HIV Long Terminal Repeat / Q000235:genetics; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008954:Models, Biological; D012333:RNA, Messenger / Q000032:analysis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014779:Virus Replication
D015704:CD4 Antigens; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D014409:Tumor Necrosis Factor-alpha; D013755:Tetradecanoylphorbol Acetate