Double fluorescent labeling method used for a study on liposomes.
Carboxyfluorescein (CF) and Calcein (CAL) have almost the same fluorescent spectrum. However, only CAL forms a nonfluorescent chelate with the Co2+ ion. It was proven that this phenomenon can be used for the fractional determination of CF and CAL. And this fractional determination method was available for a study on liposomes.
Sawahara
H
H
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Goto
S
S
Kinoshita
N
N
D000818:Animals; D005260:Female; D005452:Fluoresceins; D005453:Fluorescence; D008081:Liposomes / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR
D005452:Fluoresceins; D008081:Liposomes; C045132:4-carboxyfluorescein; C007740:fluorexon