Ascitic tumor cell cycle metabolism and energetics.
Data are presented on Ehrlich ascitic tumor cells energetic metabolism, activities of the glycolytic enzymes and the pentose phosphate pathway enzymes, contents and synthesis rates of the macromolecules at various cell cycle stages. An attempt was made to correct the direct measurement by taking into consideration a systematic error introduced in the experiment by incomplete cells synchronization. Cell metabolism activation sharply increased at two mitotic cycle stages. At the first stage (end of G1-period, beginning of S-period) the processes associated with the preparation to reduplication and DNA synthesis were activated. The second activation wave (end of S-period, G2-period) was connected with cell preparation of mitosis. The coordination of cell metabolism variations during the cell cycle is shown.
Kazmin
S D
SD
R.E. Kavetsky Institute for Oncology, Ukrainian SSR Academy of Science, Kiev.
Danko
I M
IM
Kolosov
E V
EV
Pinchouk
V G
VG
D000818:Animals; D001693:Biological Transport, Active; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002453:Cell Cycle; D002784:Cholesterol / Q000378:metabolism; D004734:Energy Metabolism; D007399:Interphase; D007939:Leukemia L1210 / Q000378:metabolism*; D007941:Leukemia P388 / Q000378:metabolism*; D008291:Malate Dehydrogenase / Q000378:metabolism; D008938:Mitosis; D009438:Neuraminic Acids / Q000378:metabolism; D009696:Nucleic Acids / Q000096:biosynthesis; D010427:Pentose Phosphate Pathway; D014176:Protein Biosynthesis; D016196:S Phase; D013936:Thymidine / Q000378:metabolism
D009438:Neuraminic Acids; D009696:Nucleic Acids; D002784:Cholesterol; D008291:Malate Dehydrogenase; D013936:Thymidine