Tissue distribution of neutral endopeptidase 24.11 ('enkephalinase') activity in guinea pig trachea.
The distribution of neutral endopeptidase 24.11 (NEP; 'enkephalinase') activity was studied on tissue sections of the guinea pig trachea using a histochemical method based on the catalytic activity of the enzyme. The specificity for NEP of the histochemical reaction was verified by application of an array of peptidase inhibitors. NEP activity was most prominent on the respiratory epithelium, but occurred also in submucous glands, connective tissue of the lamina propria, perichondrium and chondrocytes. The findings suggest that NEP in the trachea is involved in various functions, cleavage of neurally released peptides being only one of them.
Kummer
W
W
Institute for Anatomy and Cell Biology I, University of Heidelberg, Federal Republic of Germany.
Fischer
A
A
D000818:Animals; D001414:Bacitracin / Q000494:pharmacology; D002216:Captopril / Q000494:pharmacology; D004656:Enalapril / Q000494:pharmacology; D004848:Epithelium / Q000201:enzymology; D005260:Female; D006020:Glycopeptides / Q000494:pharmacology; D006168:Guinea Pigs; D007150:Immunohistochemistry; D015260:Neprilysin / Q000378:metabolism*; D015244:Thiorphan / Q000494:pharmacology; D014132:Trachea / Q000201:enzymology*
D006020:Glycopeptides; D001414:Bacitracin; D004656:Enalapril; D002216:Captopril; D015244:Thiorphan; D015260:Neprilysin; C008890:phosphoramidon