title: Establishment of a novel cell line with T-lineage phenotype (HPB-MLp-W) from a non-Hodgkin's lymphoma patient.
abstract: We report the characterization of a novel human T-cell line, HPB-MLp-W, which was established from blastic cells of a lymph node specimen from a patient with non-Hodgkin's lymphoma. They demonstrated the T-cell association antigens, CD2 and CD4, but no CD3, CD8, CD1, CD5, CD7 nor T-cell antigen receptor on their cell surfaces. They were also positive for Ia and Ki-1 antigen, and negative for CD25 (Tac-1). The cell line HPB-MLp-W had the same pattern of antigen expression as the patient's cells. Southern-blot analysis of DNA showed a rearrangement of the T-cell receptor-alpha and beta genes. To our knowledge, this is a novel cell line with unique T-lineage marker, to be established from a case of non-Hodgkin's lymphoma.
authors.affiliation: Department of Pathology, Shimane Medical University, Izumo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Morikawa
authors.forename: K
authors.initials: K
authors.lastname: Morikawa
authors.forename: J
authors.initials: J
authors.lastname: Hara
authors.forename: M
authors.initials: M
authors.lastname: Nagasaki
authors.forename: A
authors.initials: A
authors.lastname: Nakano
authors.forename: F
authors.initials: F
authors.lastname: Oseko
mesh_terms: D015703:Antigens, CD / Q000032:analysis*; D001706:Biopsy; D002460:Cell Line; D002871:Chromosome Banding; D046508:Culture Techniques / Q000379:methods; D005260:Female; D015329:Gene Rearrangement, T-Lymphocyte; D006801:Humans; D007621:Karyotyping; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D008875:Middle Aged; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology*
chemical_list: D015703:Antigens, CD