Evidence that mouse mesangial cells produce colony-stimulating factor-1.
The contractile mesangial cell has previously been shown to be a metabolically active cell capable of producing a variety of substances. In this communication, we report that the contractile mouse mesangial cell produces a factor which induces the replication of blood monocytes and splenic macrophages but not of peritoneal macrophages. This factor has an isoelectric pH of 4 as determined by chromatofocusing, a molecular weight of 68,000 daltons as determined by gel chromatography, and is reactive to anti-CSF-1 as assessed by experiments showing inhibition of function by the antibody. These physiological, physico-chemical and immunological characteristics indicate that the substance is most likely colony-stimulating factor-1 (CSF-1).
Ooi
B S
BS
Department of Internal Medicine, V.A. Medical Center, George Washington University School of Medicine, Washington, DC.
Yamashita
W
W
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002850:Chromatography, Gel; D004261:DNA Replication / Q000187:drug effects; D005920:Glomerular Mesangium / Q000502:physiology*; D007106:Immune Sera; D016173:Macrophage Colony-Stimulating Factor / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D009000:Monocytes / Q000166:cytology / Q000187:drug effects
D007106:Immune Sera; D016173:Macrophage Colony-Stimulating Factor