Tumor necrosis factor-beta in the serum of adult T-cell leukemia with hypercalcemia.
Serum tumor necrosis factor-beta (TNF-beta) from patients with adult T-cell leukemia (ATL) was studied by a sandwich enzyme-linked immunosorbent assay (ELISA) developed in our laboratory using biotinylated monoclonal anti-TNF-beta and recombinant TNF-beta. Seven of eight patients with hypercalcemia showed elevation of serum TNF-beta. On the other hand, TNF-beta could not be detected by the ELISA in 28 patients without hypercalcemia. The lower detection limit in this assay was 100 pg/mL, corresponding to 500 pg/mL by the conventional method. In two patients serum TNF-beta level decreased after treatment in association with the level of serum calcium. Furthermore, immuno-staining using anti-TNF-beta and avidin-biotin complex showed the presence of cytoplasmic TNF-beta in not only human T-cell leukemia virus type I infected cell lines, but also freshly isolated cells from ATL patients with hypercalcemia. The actual biologic activity of TNF-beta in serum was confirmed by a conventional bioassay in a patient with hypercalcemia, and its cytotoxic activity was inhibited by the addition of anti-TNF-beta antibody in the assay. These results suggested that serum TNF-beta might be one of the factors contributing to the hypercalcemia, at least in patients with ATL.
Ishibashi
K
K
Second Department of Internal Medicine, Faculty of Medicine, Kagoshima University, Japan.
Ishitsuka
K
K
Chuman
Y
Y
Otsuka
M
M
Kuwazuru
Y
Y
Iwahashi
M
M
Utsunomiya
A
A
Hanada
S
S
Sakurami
T
T
Arima
T
T
D000328:Adult; D000818:Animals; D002118:Calcium / Q000097:blood*; D002470:Cell Survival / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006934:Hypercalcemia / Q000097:blood*; D007739:L Cells / Q000166:cytology / Q000187:drug effects; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000097:blood* / Q000150:complications; D008233:Lymphotoxin-alpha / Q000097:blood* / Q000494:pharmacology; D051379:Mice; D011994:Recombinant Proteins / Q000494:pharmacology
D008233:Lymphotoxin-alpha; D011994:Recombinant Proteins; D002118:Calcium