title: Nonradioactive DNA probe for detection of gene for methicillin resistance in Staphylococcus aureus.
abstract: A DNA probe was developed by inserting, in the SmaI site of pBluescript sK, a 528-bp fragment of the gene responsible for intrinsic resistance to beta-lactam antibiotics in Staphylococcus aureus (mec determinant). The mec probe provided a useful tool for the rapid identification of the intrinsic resistance trait and for establishing guidelines for testing the in vitro susceptibility of S. aureus to beta-lactams.
authors.affiliation: Institute of Microbiology, University of Verona, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Ligozzi
authors.forename: G M
authors.initials: GM
authors.lastname: Rossolini
authors.forename: E A
authors.initials: EA
authors.lastname: Tonin
authors.forename: R
authors.initials: R
authors.lastname: Fontana
mesh_terms: D015342:DNA Probes; D006824:Hybridization, Genetic; D008712:Methicillin / Q000494:pharmacology*; D016106:Methicillin Resistance / Q000235:genetics*; D015183:Restriction Mapping; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics; D001618:beta-Lactamases / Q000378:metabolism
chemical_list: D015342:DNA Probes; D001618:beta-Lactamases; D008712:Methicillin