Scaling up in isolation of medium-size uraemic toxins.
Plasmatic accumulation of uraemic toxins in the middle molecular mass range has been reported to be associated with several pathologies observed in uraemic patients. The very low concentration of these toxins in uraemic body fluids makes classical chromatography techniques inadequate in isolating sufficient amounts of these endogenous substances, thus precluding their identification. A scaling up of gel permeation and ion-exchange chromatographies was therefore developed. This considerably increased the amount of uraemic toxins isolated, thus allowing the study of their chemical nature and facilitating understanding of their biological activities.
Gallice
P M
PM
Laboratoire de Biophysique, Faculté de Pharmacie, Marseille, France.
Crevat
A D
AD
Berland
Y F
YF
D002850:Chromatography, Gel / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006801:Humans; D014118:Toxins, Biological / Q000097:blood / Q000302:isolation & purification*
D014118:Toxins, Biological; C026865:uremia middle molecule toxins