A new system for high-resolution DNA sequence mapping interphase pronuclei.
Cosmid clones containing human or hamster inserts have been hybridized in situ and localized with fluorescent reporter molecules in interphase nuclei (pronuclei) obtained after fusion of hamster eggs with either human or hamster sperm. Hamster egg cytoplasm processes the tightly packaged sperm DNA into large diffuse networks of chromatin fiber bundles, providing hybridization targets more extended than those available in somatic interphase cell nuclei. Pronuclear physical distances between hybridization signals were measured in micrometers and correlated to genomic distances determined by restriction fragment analyses, using cosmids from the Chinese hamster DHFR region and from the human Factor VIII/color vision pigment gene region (Xq28). The mean pronuclear distances between hybridization sites were about three times as large as those measured in somatic interphase cells for equivalent genomic distances. The relationship between physical and genomic distances was linear from less than 50 kb to at least 800 kb. The results show that physical distance in the sperm-egg system promises to extend the mapping range obtainable in somatic interphase nuclei below 50 kb and up to at least 800 kb.
Brandriff
B
B
Lawrence Livermore National Laboratory, University of California, Livermore, California 94550.
Gordon
L
L
Trask
B
B
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D002874:Chromosome Mapping / Q000379:methods*; D002888:Chromosomes, Human, Pair 19; D003001:Cloning, Molecular; D003360:Cosmids; D006224:Cricetinae; D004247:DNA / Q000378:metabolism; D015342:DNA Probes; D005260:Female; D006801:Humans; D007399:Interphase; D008297:Male; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization; D010063:Ovum / Q000648:ultrastructure; D013094:Spermatozoa / Q000648:ultrastructure; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics; D014960:X Chromosome
D015342:DNA Probes; D004247:DNA; D013762:Tetrahydrofolate Dehydrogenase