Oxidative modification of glycated low density lipoprotein in the presence of iron.
This is the first observation for contributing to the glycation of low density lipoprotein (LDL) to oxidative modification of its own lipids and protein. Human plasma LDL was glycated by incubation with glucose (G-LDL). Glucose incorporated into apoprotein B was approximately 10 mol/mol of apoprotein (2.8% modification of lysine residues) and 84% of G-LDL was adsorbed on phenylboronate affinity column. G-LDL incubated with Fe3+ for 4 h caused a significantly higher level of lipid peroxidation than U-LDL (untreated with glucose), and a higher molecular weight protein was observed in apoprotein B on SDS-polyacrylamide gel electrophoresis (SDS-PAGE), increasing with incubation period. Corresponding to change on SDS-PAGE, G-LDL exposed to Fe3+ moved faster than G-LDL per se or U-LDL to anode on agarose gel electrophoresis. The higher the Fe3+ concentration, the more lipid peroxidation was caused. Alpha-tocopherol or probucol suppressed the lipid peroxidation of G-LDL exposed to Fe3+.
Sakurai
T
T
Tokyo College of Pharmacy, Tokyo, Japan.
Kimura
S
S
Nakano
M
M
Kimura
H
H
D004591:Electrophoresis, Polyacrylamide Gel; D005947:Glucose / Q000378:metabolism; D017127:Glycation End Products, Advanced; D006801:Humans; D007501:Iron / Q000494:pharmacology*; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects*; D008077:Lipoproteins, LDL / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D010084:Oxidation-Reduction; D011341:Probucol / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology; D000093844:Glycated Proteins
D005947:Glucose; D017127:Glycation End Products, Advanced; D007501:Iron; D008077:Lipoproteins, LDL; D011341:Probucol; D014810:Vitamin E; C031797:glycated lipoproteins, LDL; D000093844:Glycated Proteins