A novel spermidine-dependent endoribonuclease activity caused by RNA-protein complex in mouse FM3A cell extracts.
We have found a novel spermidine-dependent endoribonuclease activity in mouse FM3A cell extracts. This endoribonuclease cleaves RNA substrates containing a sequence CCCCCGGUUUGU in its middle. This activity is lost either by heat- or micrococcal nuclease-pretreatment. When heat-pretreated extracts and micrococcal nuclease-pretreated ones are mixed, the activity is restored, suggesting that this activity requires both RNA and protein components. Testing the restoration of the lost endoribonuclease activity in micrococcal nuclease-pretreated extracts by addition of fractionated cellular RNAs, we identified an approximately 65 nucleotide RNA required for this endoribonuclease activity.
Nashimoto
M
M
Department of Biochemistry, Niigata University School of Medicine, Japan.
Kominami
R
R
Nishi
S
S
Mishima
Y
Y
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004722:Endoribonucleases / Q000378:metabolism*; D051379:Mice; D008836:Micrococcal Nuclease / Q000378:metabolism; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis; D013095:Spermidine / Q000494:pharmacology*; D013379:Substrate Specificity; D013698:Templates, Genetic; D014158:Transcription, Genetic
D009841:Oligonucleotides; D004722:Endoribonucleases; C070374:ribonuclease 65; D008836:Micrococcal Nuclease; D013095:Spermidine