Influence of the incorporation of (S)-9-(3,4-dihydroxybutyl)adenine on the enzymatic stability and base-pairing properties of oligodeoxynucleotides.
(S)-9-(3,4-dihydroxybutyl)adenine was used at several positions as nucleoside substitute in the synthesis of dimers and 13-mers. Therefore we used the phosporamidite and the H-phosphonate chemistry. The nuclease susceptibilities and the base-pairing properties of these oligomers have been evaluated.
Augustyns
K
K
Rega Institute for Medical Research, Leuven, Belgium.
Van Aerschot
A
A
Van Schepdael
A
A
Urbanke
C
C
Herdewijn
P
P
D000225:Adenine / Q000031:analogs & derivatives* / Q000737:chemistry; D001482:Base Composition; D004795:Enzyme Stability; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000378:metabolism; D011108:Polymers / Q000138:chemical synthesis / Q000378:metabolism; D013696:Temperature
D009838:Oligodeoxyribonucleotides; D011108:Polymers; C069128:9-(3,4-dihydroxybutyl)adenine; D000225:Adenine