title: Interphase cytogenetics reveals somatic pairing of chromosome 17 centromeres in normal human brain tissue, but no trisomy 7 or sex-chromosome loss.
abstract: Nuclei isolated from normal human brain tissue, collected from six autopsies, were hybridized with a panel of nine satellite DNA probes specific for the centromeric regions of chromosomes 1, 6, 7, 10, 11, 17, 18, and the X and Y chromosomes. The results did not confirm the recently reported trisomy 7 and loss of sex chromosomes observed in metaphases obtained from normal brain tissue after short-term cultures; however, cells of all six brains displayed somatic pairing of the chromosome 17 centromeres in approximately 50% of the nuclei.
authors.affiliation: Department of Neurology, Leiden University, The Netherlands.
authors.forename: E P
authors.initials: EP
authors.lastname: Arnoldus
authors.forename: I A
authors.initials: IA
authors.lastname: Noordermeer
authors.forename: A C
authors.initials: AC
authors.lastname: Peters
authors.forename: A K
authors.initials: AK
authors.lastname: Raap
authors.forename: M
authors.initials: M
authors.lastname: Van der Ploeg
mesh_terms: D001921:Brain / Q000648:ultrastructure*; D002886:Chromosomes, Human, Pair 17 / Q000648:ultrastructure*; D002897:Chromosomes, Human, Pair 7; D003582:Cytogenetics / Q000379:methods*; D015342:DNA Probes; D005260:Female; D006801:Humans; D007399:Interphase; D008297:Male; D009693:Nucleic Acid Hybridization; D012730:Sex Chromosomes / Q000648:ultrastructure*; D014314:Trisomy
chemical_list: D015342:DNA Probes