Crystallization and preliminary diffraction analysis of cholesterol esterase from Candida cylindracea.
Cholesterol esterase (EC 3.1.1.13) from the microorganism Candida cylindracea has been crystallized in two forms. Crystals, typically 0.30 x 0.15 x 0.10 mm in size, diffract rotating anode generated x-rays to beyond 3 A are suitable for data collection for an x-ray crystallographic investigation. A monoclinic crystal form in the space group P2(1) was found to have cell dimensions of a = 122.9 A, b = 101.0 A, c = 95.2 A and beta = 108.3 degrees. The asymmetric unit of the cell contains two dimers of 129 kDa each. A second crystal form, in the triclinic space group P1, has cell dimensions of a = 58.6 A, b = 88.7 A, c = 58.6 A, alpha = 93.3 degrees, beta = 113.8 degrees and gamma = 96.0 degrees, and has one dimer per asymmetric unit.
Ghosh
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Medical Foundation of Buffalo, Inc., NY 14203-1196.
Erman
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Duax
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D002175:Candida / Q000201:enzymology*; D003460:Crystallization; D002787:Sterol Esterase / Q000737:chemistry / Q000302:isolation & purification*; D014961:X-Ray Diffraction
D002787:Sterol Esterase