title: Mapping irradiation hybrids to cosmid and yeast artificial chromosome libraries by direct hybridization of Alu-PCR products.
abstract: A direct hybridization protocol is described for screening cosmid and yeast artificial chromosome libraries with pools of Alu-PCR products from somatic cell or irradiation hybrids. This method eliminates purification, cloning and analysis of each individual Alu-PCR product before library screening. A series of human X chromosome irradiation hybrids were mapped by this method, using a cosmid reference library for comparisons between overlapping hybrids to identify interesting clones for further analysis.
authors.affiliation: Genome Analysis Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London, UK.
authors.forename: A P
authors.initials: AP
authors.lastname: Monaco
authors.forename: V M
authors.initials: VM
authors.lastname: Lam
authors.forename: G
authors.initials: G
authors.lastname: Zehetner
authors.forename: G G
authors.initials: GG
authors.lastname: Lennon
authors.forename: C
authors.initials: C
authors.lastname: Douglas
authors.forename: D
authors.initials: D
authors.lastname: Nizetic
authors.forename: P N
authors.initials: PN
authors.lastname: Goodfellow
authors.forename: H
authors.initials: H
authors.lastname: Lehrach
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D003360:Cosmids; D004247:DNA; D015723:Gene Library; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D014960:X Chromosome
chemical_list: D004247:DNA