Cloning of nocardioform DNA conferring the ability to inactivate rifampicin.
A novel mechanism of resistance to rifampicin has recently been reported in which this antibiotic is inactivated. Here we describe the cloning of DNA from a nocardiofrom strain conferring this ability to inactivate rifampicin. Cloning was on the basis of complementation, as an increased resistance to the antibiotic. The genetic information was on a 8.3-kb BglII fragment.
Andersen
S J
SJ
Department of Genetics, University of the Witwatersrand, Johannesburg, Republic of South Africa.
Dabbs
E R
ER
D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D009154:Mutation; D010957:Plasmids; D015183:Restriction Mapping; D012240:Rhodococcus / Q000187:drug effects / Q000235:genetics*; D012293:Rifampin / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D012293:Rifampin