title: Variability in LPS composition, antigenicity and reactogenicity of phase variants of Bordetella pertussis.
abstract: Comparison of lipopolysaccharides (LPS) from phase variants of different strains of Bordetella phase variants of different strains of Bordetella pertussis has shown a difference in their composition, antigenicity and reactogenicity. Phase I variants of B. pertussis, with the exception of strain 134, contain a preponderance of LPS I whereas the major component of LPS of phase IV variants is LPS II. Sera raised to LPSs of phase I strains, other than 134, cross-react with each other but not with phase IV LPSs; and similarly all sera raised to phase IV LPSs cross-react with each other and with LPS from 134 phase I. The LPSs of all phase I variants, including that of 134, are approximately ten-fold or more reactive in the limulus amoebocyte lysate assay (LAL) than phase IV LPSs. In the human mononuclear cell pyrogen assay phase IV LPSs also stimulated a lower response than phase I LPSs. The B. pertussis phase I LPSs are 10-times more reactive than Escherichia coli standard endotoxin in the LAL assay but 100-times less reactive than E. coli LPS in the monocyte test for pyrogen. The SDS-PAGE profiles of B. pertussis LPSs are quite different from those of B. parapertussis and B. bronchiseptica strains. B. pertussis LPSs produced a typical lipo-oligosaccharide (LOS) pattern. B. bronchiseptica LPS produced a similar pattern but was antigenically distinct from B. pertussis LPSs I and II. B. parapertussis in contrast produced a ladder pattern typical of smooth type LPS.
authors.affiliation: Division of Bacteriology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, U.K.
authors.forename: A
authors.initials: A
authors.lastname: Ray
authors.forename: K
authors.initials: K
authors.lastname: Redhead
authors.forename: S
authors.initials: S
authors.lastname: Selkirk
authors.forename: S
authors.initials: S
authors.lastname: Poole
mesh_terms: D000818:Animals; D001886:Bordetella pertussis / Q000032:analysis* / Q000254:growth & development / Q000276:immunology; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000032:analysis*; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D008033:Limulus Test; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000276:immunology / Q000633:toxicity; D051379:Mice; D009000:Monocytes / Q000378:metabolism; D011749:Pyrogens / Q000032:analysis
chemical_list: D004731:Endotoxins; D008070:Lipopolysaccharides; D011749:Pyrogens