Optimum associations of tester strains for maximum detection of mutagenic compounds in the Ames test.
The Ames test is now widely used as a short-term test for the detection of mutagens. Different strains are available with various genetic characteristics, and in the past decade various authors have recommended different associations of strains to give maximum detection potential. However, few studies have been done to compare the sensitivity of individual strains towards a wide range of compounds in a single study. In order to define the best association of strains for screening or regulatory purpose, we have tested 103 direct mutagens (reference genotoxins or in-house compounds) on 7 strains of Salmonella typhimurium: TA1535, TA1537, TA1538, TA97, TA98, TA100 and TA102. 126 different associations of strains have been studied in terms of sensitivity and percentage overlap. Optimum associations of 2, 3, 4 or 5 strains included strains both with and without plasmid pKM101. However, the specificity of detection is greatly diminished by the presence of plasmid pKM101 in the strain, as shown by the high degree of overlap in associations constituted entirely of strains containing the plasmid. The association of strains TA1538 and TA100 detected 86% of the chemicals tested and is therefore recommended for large-scale screening. A rate of detection of 100% was obtained when 6 strains were used. The best associations of 4 and 5 strains, which detected 97 and 99% chemicals respectively, all contained strains TA1537, TA1538 and TA102. Finally, the associations of 4 strains (TA1537, TA1538, TA100, TA102) or 5 strains (TA1535, TA1537, TA1538, TA97, TA102) seemed well adapted to the optimum detection of mutagenic compounds.
Bonneau
D
D
Centre de Recherche de Vitry-Alfortville, Institut de Recherche sur la Sécurité du Médicament, Vitry sur Seine, France.
Thybaud
V
V
Melcion
C
C
Bouhet
F
F
Cordier
A
A
D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens; D010957:Plasmids; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics*; D012680:Sensitivity and Specificity
D009153:Mutagens