title: Method for the characterization of stationary phases for the separation of proteins by high-performance liquid chromatography.
abstract: A conformational change of lysozyme and myoglobin can be visualized with transition curves in a high-performance liquid chromatographic (HPLC) system using a diode-array detector and UV difference spectrometry. Transition temperatures calculated from the curves are affected by the mobile phase composition and the column packing material. Flow-rates as used in HPLC do not have a measurable influence.
authors.affiliation: Laboratory for Analytical Chemistry, University of Amsterdam, The Netherlands.
authors.forename: R E
authors.initials: RE
authors.lastname: Huisden
authors.forename: J C
authors.initials: JC
authors.lastname: Kraak
authors.forename: H
authors.initials: H
authors.lastname: Poppe
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D009113:Muramidase / Q000302:isolation & purification; D009211:Myoglobin / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
chemical_list: D009211:Myoglobin; D011506:Proteins; D012997:Solvents; D009113:Muramidase