[Increasing the sensitivity of in vitro transformed hamster embryo cells (STHE strain) to cytolysis by resident and activated macrophages].
The sensitivity of low-malignant spontaneously in vitro transformed hamster embryo cells (STHE strain) to cytolysis of both resident and activated macrophages has been examined with cytolytic 3H-thymidine release assay. Activated macrophages were obtained from Syrian hamster peritoneal exudate cells 5 days following priming with 3% thioglycollate-broth and subsequent in vitro activation with proper-myl, levan, LPS, MDP, 1,4-dihydropyridine-derivate PP-256 and PMA. It has been shown that the STHE strain cells were sensitive to cytolysis by only fully activated macrophages. Both resident and non-activated (Thioglycollate-elicited) macrophages have not developed significant levels of the cytolytic activity against STHE cell targets. Short-term treatment of STHE cells with actinomycin D result in augmentation of their sensitivity to cytolysis by resident and activated macrophages.
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D000276:Adjuvants, Immunologic; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000473:pathology*; D006224:Cricetinae; D003470:Culture Media; D003602:Cytotoxicity, Immunologic / Q000187:drug effects / Q000276:immunology*; D003609:Dactinomycin / Q000494:pharmacology*; D004622:Embryo, Mammalian / Q000473:pathology*; D066298:In Vitro Techniques; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D009374:Neoplasms, Experimental / Q000276:immunology / Q000473:pathology*
D000276:Adjuvants, Immunologic; D003470:Culture Media; D003609:Dactinomycin
Povyshenie chuvstvitel'nosti transformirovannykh in vitro kletok shtamma KhETR k tsitoliticheskomu deĭstviiu rezidentnykh i aktivirovannykh makrofagov.