cDNA cloning of porcine interleukin 2 by polymerase chain reaction.
Porcine interleukin 2 (IL-2) cDNA was cloned by polymerase chain reaction (PCR), using primers derived from the corresponding bovine sequence. The resulting porcine DNA sequence encodes a 154 residue IL-2 primary translation product. Comparison of the mature, secreted form of porcine IL-2 with those of other species was carried out in an attempt to identify differences that might contribute to the observed differing species specificities.
Goodall
J C
JC
Department of Biochemistry, University of Bristol, U.K.
Emery
D C
DC
Bailey
M
M
English
L S
LS
Hall
L
L
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D015375:Receptors, Interleukin-2 / Q000235:genetics / Q000378:metabolism; D012756:Sheep; D013045:Species Specificity; D013552:Swine
D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D004247:DNA