Electrophoretic separation of 5'-nucleotidase multiple forms.
A procedure is proposed for the separation of multiple forms of 5'-nucleotidase (EC 3.1.3.5) by cellulose acetate electrophoresis. The effects of various treatments (wheat-germ lectin, neuraminidase, n-butanol, papain, Triton X-100 and precipitation of LDL and VLDL) on the electrophoretic pattern of 5'-nucleotidase and alkaline phosphatase were studied. In healthy controls, the presence of three fractions with alpha 1, alpha 2 and beta mobilities was observed, the latter being the major one. In different hepatobiliary diseases a close relationship between the presence of high molecular weight alkaline phosphatase and the increase in the ratio Total/beta 5'-nucleotidase was observed, showing that the increase in total 5'-nucleotidase in these patients is mainly due to the alpha 1 isoform. The nature of these forms is discussed.
Novo
F J
FJ
Laboratorio Central, Hospital General de Galicia, Santiago de Compostela, Spain.
Tutor
J C
JC
D015720:5'-Nucleotidase / Q000302:isolation & purification* / Q000378:metabolism / Q000652:urine; D000469:Alkaline Phosphatase / Q000302:isolation & purification / Q000378:metabolism; D001646:Bile / Q000378:metabolism; D000440:Butanols; D011232:Chemical Precipitation; D003902:Detergents; D004588:Electrophoresis, Cellulose Acetate; D006801:Humans; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000302:isolation & purification; D008079:Lipoproteins, VLDL / Q000302:isolation & purification; D008107:Liver Diseases / Q000378:metabolism; D009439:Neuraminidase; D017830:Octoxynol; D010206:Papain; D011092:Polyethylene Glycols; D010738:Type C Phospholipases; D014909:Wheat Germ Agglutinins
D000440:Butanols; D003902:Detergents; D007527:Isoenzymes; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D014909:Wheat Germ Agglutinins; D011092:Polyethylene Glycols; D017830:Octoxynol; D000469:Alkaline Phosphatase; D015720:5'-Nucleotidase; D010738:Type C Phospholipases; D009439:Neuraminidase; D010206:Papain