title: Effects of 2,4-dichlorophenoxyacetic acid and picloram on biotransformation, peroxisomal and serum enzyme activities in channel catfish (Ictalurus punctatus).
abstract: Channel catfish (Ictalurus punctatus) exposed to a mixture of picloram and 2,4-dichlorophenoxyacetic acid (2,4-D) for 10 days displayed increased activities of hepatic ethoxyresorufin O-deethylase (EROD), decreased serum chloride concentrations and decreased liver/body weight ratios. These changes were not observed in fish exposed to either compound alone. Neither compound, nor their mixture, increased hepatic peroxisomal catalase or lauroyl CoA oxidase activities. The results of this study indicate that 10-day exposure of 2,4-D or picloram does not induce peroxisomal enzymes in channel catfish; however, exposure to a mixture of 2,4-D and picloram may cause physiological effects to catfish not observed with either compound alone.
authors.affiliation: Ecotoxicology Laboratory, School of Forestry and Environmental Studies, Duke University, Durham, NC 27706.
authors.forename: E P
authors.initials: EP
authors.lastname: Gallagher
authors.forename: R T
authors.initials: RT
authors.lastname: Di Giulio
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000633:toxicity*; D000214:Acyl Coenzyme A / Q000032:analysis; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002374:Catalase / Q000032:analysis; D002712:Chlorides / Q000097:blood; D019363:Cytochrome P-450 CYP1A1; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis / Q000097:blood; D005982:Glutathione Transferase / Q000032:analysis; D007059:Ictaluridae / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008830:Microbodies / Q000187:drug effects / Q000201:enzymology; D009929:Organ Size / Q000187:drug effects; D010088:Oxidoreductases / Q000032:analysis / Q000097:blood; D010846:Picloram / Q000633:toxicity*
chemical_list: D000214:Acyl Coenzyme A; D002712:Chlorides; D015084:2,4-Dichlorophenoxyacetic Acid; C050466:lauroyl-coenzyme A; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D002374:Catalase; D019363:Cytochrome P-450 CYP1A1; D005982:Glutathione Transferase; D010846:Picloram