title: Lead speciation by HPLC-ICP-AES and HPLC-ICP-MS.
abstract: Speciation of inorganic lead (Pb2+) and several trialkyllead species (trimethyllead chloride [TML], triethyllead chloride [TEL], and triphenyllead chloride [TPhL]) is investigated using high-performance liquid chromatography (HPLC) with detection by both inductively coupled plasma emission spectroscopy (ICP-AES), and inductively coupled plasma mass spectrometry (ICP-MS). Reversed-phase, ion-pairing, and ion-exchange HPLC modes are studied. Optimal chromatographic conditions for ICP-AES detection include a reversed-phase separation utilizing a step gradient from 10 to 70% methanol. However, the gradient has been found to destabilize the plasma when using ICP-MS detection. An isocratic separation with a 30% methanol mobile phase has been found to be the best compromise between plasma stability and chromatographic resolution. Detection limits using ICP-MS detection are 3 orders of magnitude improved over ICP-AES detection.
authors.affiliation: Department of Chemistry, University of Cincinnati, Ohio 45221.
authors.forename: A
authors.initials: A
authors.lastname: al-Rashdan
authors.forename: D
authors.initials: D
authors.lastname: Heitkemper
authors.forename: J A
authors.initials: JA
authors.lastname: Caruso
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D007854:Lead / Q000032:analysis*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D000432:Methanol; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods*
chemical_list: D007854:Lead; D000432:Methanol