Screening of various mammalian cell culture media to establish a downstream purification scheme.
Various serum-free media for use in hybridoma cell cultures are reviewed and their protein composition analysed. The chromatographic behaviour of the serum-free components was analysed on ion exchange, hydrophobic and sizing columns in order to facilitate the design of a purification scheme for monoclonal antibodies produced from various cell cultures.
Graf
H
H
Setric Génie Industriel, S.G.I., Toulouse, France.
Rabaud
J N
JN
Egly
J M
JM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange; D003470:Culture Media / Q000032:analysis*; D003584:Cytological Techniques; D006801:Humans; D006825:Hybridomas / Q000166:cytology; D011506:Proteins / Q000032:analysis; D012995:Solubility
D000911:Antibodies, Monoclonal; D003470:Culture Media; D011506:Proteins