A side-entry cold holder for cryo-electron microscopy.
A side-entry cold holder for cryo-electron microscopy has been constructed which can be used with no loss of image resolution or contrast at 3.4 A resolution compared to the normal room-temperature holder. A description is presented of some of the ideas and tests which we have developed during its construction and are included in the design, together with a briefer outline of the remaining limitations.
Henderson
R
R
Medical Research Council, Laboratory of Molecular Biology, Cambridge, UK.
Raeburn
C
C
Vigers
G
G
D015925:Cryopreservation; D008854:Microscopy, Electron / Q000379:methods*