Autoantibodies directed against lipid membrane components in sera of patients with malignant tumors.
Tumor-associated antigens (Ag) are expressed on neoplastic cells. Using an enzyme-linked immunosorbent assay (ELISA), an attempt was made to evaluate the autoimmune responses directed against lipid membrane components. A comparison was made of autoantibody (autoAb) levels in human sera of 684 patients with malignant tumors and those of 185 controls (healthy subjects and patients suffering from other diseases). A highly significant difference was found between the immunological binding of the groups for only one phospholipids (PL), i.e., phosphatidylinositol (PI). Using ELISA tests and PI-related compounds differing in fatty acid residue types and/or in phosphatidyl group, it was demonstrated that the hydrophilic residue is the immunodominant part recognized by the autoAb detected in sera of cancer patients.
Faiderbe
S
S
Immunology Laboratory, IBCN-CNRS, Bordeaux, France.
Chagnaud
J L
JL
Charrier
M C
MC
Peyron
M A
MA
Wafflart
J
J
Geffard
M
M
D000328:Adult; D001323:Autoantibodies / Q000032:analysis*; D001943:Breast Neoplasms / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008563:Membrane Lipids / Q000276:immunology*; D008875:Middle Aged; D009369:Neoplasms / Q000276:immunology*; D010743:Phospholipids / Q000276:immunology*; D012680:Sensitivity and Specificity
D001323:Autoantibodies; D008563:Membrane Lipids; D010743:Phospholipids