title: The sequence of human immunodeficiency virus type 2 circle junction suggests that integration protein cleaves the ends of linear DNA asymmetrically.
abstract: The sequence of the human immunodeficiency virus type 2 circle junction was determined. The most common sequence found between the conserved CA and TG dinucleotides at the ends of the integrated provirus was five bases long (GGTAC). This suggests that the integration of human immunodeficiency virus type 2 DNA is accompanied by the asymmetric loss of two and three bases, respectively, from the U3 and U5 ends of the linear double-stranded DNA prior to integration.
authors.affiliation: ABL-Basic Research Program, National Cancer Institute-Frederick Cancer Research and Development Center, Maryland 21702-1201.
authors.forename: J M
authors.initials: JM
authors.lastname: Whitcomb
authors.forename: S H
authors.initials: SH
authors.lastname: Hughes
mesh_terms: D001483:Base Sequence; D002472:Cell Transformation, Viral; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D015498:HIV-2 / Q000235:genetics*; D019426:Integrases; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D009841:Oligonucleotides; D004254:DNA Nucleotidyltransferases; D019426:Integrases