DNA bioprints: simple nonisotopic DNA fingerprints with biotinylated probes.
The discovery of multilocal DNA fingerprinting represented a revolution in criminal identification and paternity testing. However, for routine use in clinical laboratories, the standard DNA fingerprint methodology is too complex. We have been successful in the development of a simplified DNA nonisotopic fingerprinting system using biotin-labeled probes which we have called DNA bioprints. To achieve this we explored three main technical features: utilization of biotinylated nonradioactive probes as a simpler substitute for 32P-labeled probes, utilization of oligonucleotide probes as a simpler substitute for recombinant probes, and direct hybridization in the dried agarose gel as a simpler substitute for Southern blots. In this article we review our results in the development of DNA bioprints.
Pena
S D
SD
Department of Biochemistry, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Macedo
A M
AM
Gontijo
N F
NF
Medeiros
A M
AM
Ribeiro
J C
JC
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D001710:Biotin; D016172:DNA Fingerprinting; D015342:DNA Probes; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010375:Pedigree; D019809:Streptavidin
D001426:Bacterial Proteins; D015342:DNA Probes; D015345:Oligonucleotide Probes; D001710:Biotin; D019809:Streptavidin