[Beneficial effects of fructose-1,6-diphosphate infusion on hepatic regeneration after partial hepatectomy by microwave tissue coagulator].
Experiments were performed to explore the effect of microwave tissue coagulator (MTC) on post-lobectomy regeneration of the liver and the regeneration-promoting effect of fructose-1,6-diphosphate (FDP) on heat injury of the liver after 40% hepatectomy in rats. Concerning the effect of MTC on hepatic regeneration, a MTC group showed a significantly higher serum LDH level on post-operative day 2, compared with a simple ligation group. A significant difference in DNA synthesizing activity between two groups was noted only in the peripheral region of regenerating liver tissue. A marked depression was observed on post-operative day 2 in the MTC group, 433 +/- 50 dpm/micrograms DNA as compared to 782 +/- 111 dpm/micrograms DNA in the simple ligation group, and also in respect of protein synthesis and labeling index. Administration of FDP prior to operation brought about a complete inhibition of the serum LDH elevation. DNA synthesis was significantly enhanced in the peripheral region of regenerating liver tissue in rats pretreated with FDP (422 +/- 52, 783 +/- 112 and 912 +/- 115 dpm/micrograms DNA in the saline, FDP 0.25 mmol/kg and FDP 0.8 mmol/kg groups, respectively). Similar trends were observed as to protein synthesis and labeling index. FDP has been proven to be effective on protection of the liver cells as well as on promotion of the liver regeneration following hepatic resection by MTC.
Nakai
T
T
Department of Gastroenterological Surgery, Wakayama Medical College, Japan.
Tanimura
H
H
Tabuse
K
K
Nagai
Y
Y
Mori
K
K
Yamoto
H
H
D000818:Animals; D004247:DNA / Q000096:biosynthesis; D005635:Fructosediphosphates / Q000008:administration & dosage / Q000494:pharmacology*; D006498:Hepatectomy / Q000379:methods; D007262:Infusions, Intravenous; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D008115:Liver Regeneration / Q000187:drug effects / Q000528:radiation effects*; D008297:Male; D008872:Microwaves / Q000009:adverse effects*; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains
D005635:Fructosediphosphates; D004247:DNA; C029063:fructose-1,6-diphosphate