Distribution of estrogen receptor-immunoreactive cells in the forebrain of the female guinea pig.
We mapped the distribution of estrogen receptor-containing cells in the forebrain of the adult female guinea pig. Cellular estrogen receptor content was detected using monoclonal antibody H222, directed against the estrogen receptor, and the avidin-biotin method with nickel-intensified diaminobenzidine as the chromagen. A complete set of deletion, titration, and adsorption controls established the specificity of the staining. The most dense collections of estrogen receptor-immunoreactive cells were found in medial preoptic, medial hypothalamic, and limbic nuclei (amygdala, bed nucleus of the stria terminalis, lateral septum). Numerous estrogen receptor-immunoreactive cells were also found in additional, specific subregions of the remainder of the preoptic area, hypothalamus, and limbic system, and also in the midbrain (central gray). Elsewhere, estrogen receptor-immunoreactive cells were present in smaller numbers or were absent. This map confirms and extends previous maps based on estrogen binding. The majority of estrogen receptor-immunoreactive cells are found in areas known to be involved in some aspect of reproduction. In addition, many estrogen receptor-immunoreactive cells are found in areas not typically considered to have a primary role in reproductive behavior or neuroendocrine function.
DonCarlos
L L
LL
Institute of Animal Behavior, Rutgers University, Newark, New Jersey 07102.
Monroy
E
E
Morrell
J I
JI
D000818:Animals; D001931:Brain Mapping / Q000379:methods; D004027:Diencephalon / Q000737:chemistry*; D005260:Female; D006168:Guinea Pigs / Q000378:metabolism*; D007031:Hypothalamus / Q000737:chemistry; D007150:Immunohistochemistry; D008636:Mesencephalon / Q000737:chemistry; D011960:Receptors, Estrogen / Q000032:analysis*; D013687:Telencephalon / Q000737:chemistry*; D009626:Terminology as Topic; D013788:Thalamus / Q000737:chemistry
D011960:Receptors, Estrogen