title: Interleukin-6 does not stimulate bone resorption in neonatal mouse calvariae.
abstract: Recombinant human interleukin-6 (IL-6) was assessed for its ability to stimulate bone resorption in prelabeled mouse calvariae in vitro. IL-6 had no effect on bone resorption at concentrations ranging from 300 to 10,000 U/ml (3-1000 pg/ml). Neither the presence of indomethacin nor prolonged incubation periods (96 h) affected this result. IL-6 did not affect resorption stimulated by human recombinant IL-1 alpha (rIL-1 alpha) but inhibited resorption stimulated by parathyroid hormone (PTH) and 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3]. rIL-1 alpha, PTH, and 1,25-(OH)2D3 induced IL-6 release by calvariae. We conclude from these studies that IL-6 does not stimulate bone resorption in neonatal mouse calvariae. However, it may act as a locally produced inhibitor and therefore a paracrine regulator of bone resorption induced by osteotropic hormones. IL-6 could also function as a long-range stimulator of systemic reactions and acute-phase responses to local inflammatory and neoplastic lesions in bone.
authors.affiliation: Department of Human Metabolism and Clinical Biochemistry, Sheffield University Medical School, UK.
authors.forename: A
authors.initials: A
authors.lastname: al-Humidan
authors.forename: S H
authors.initials: SH
authors.lastname: Ralston
authors.forename: D E
authors.initials: DE
authors.lastname: Hughes
authors.forename: K
authors.initials: K
authors.lastname: Chapman
authors.forename: L
authors.initials: L
authors.lastname: Aarden
authors.forename: R G
authors.initials: RG
authors.lastname: Russell
authors.forename: M
authors.initials: M
authors.lastname: Gowen
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D001862:Bone Resorption / Q000139:chemically induced*; D002117:Calcitriol / Q000494:pharmacology; D046508:Culture Techniques; D004357:Drug Synergism; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology*; D051379:Mice; D010281:Parathyroid Hormone / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D012886:Skull / Q000187:drug effects; D000093242:Interleukin-6 Inhibitors
chemical_list: D002117:Calcitriol; D007213:Indomethacin; D007375:Interleukin-1; D015850:Interleukin-6; D010281:Parathyroid Hormone; D011994:Recombinant Proteins; D000093242:Interleukin-6 Inhibitors