Physical mapping of the Mycoplasma capricolum genome.
A physical map of Mycoplasma capricolum ATCC 27343 genome was constructed, based on estimation of the restriction fragment sizes by pulse-field electrophoresis. The linkage order of restriction fragments was determined by two-dimensional electrophoresis of partial and complete single digests and complete double digests and by Southern hybridization analysis. The genome size was established at 1155.5 kb, and 26 cleavage sites for 7 endonucleases were assigned to the map.
Miyata
M
M
Department of Biology, Faculty of Science, Osaka City University, Japan.
Wang
L
L
Fukumura
T
T
D015139:Blotting, Southern; D002874:Chromosome Mapping; D015180:Electrophoresis, Gel, Two-Dimensional; D005798:Genes, Bacterial; D008040:Genetic Linkage / Q000235:genetics; D009174:Mycoplasma / Q000235:genetics*; D015183:Restriction Mapping