title: Occurrence of ceramide digalactoside as the main glycosphingolipid in the marine sponge Halichondria japonica.
abstract: The main glycosphingolipid of the sponge Halichondria japonica was isolated and its structure was determined to be ceramide digalactoside, Gal alpha 1----4Gal beta 1----1Cer, using FAB/MS, IR, 1H-NMR and chemical methods such as permethylation analysis and degradation techniques. The ceramide moiety was composed mainly of 4-hydroxy-iso-octadecasphinganine and 2-hydroxy-docosanoic acid. Interestingly, the branched long-chain bases were comprised of 70% total long-chain bases and 17.5% 4-hydroxy-anteiso-nonadecasphinganine. Ceramide monohexoside, consisting of galactosyl ceramide (81%) and glucosyl ceramide (19%), was also isolated as a minor component. The composition of the ceramide moiety was nearly the same as that of ceramide digalactoside.
authors.affiliation: Department of Chemistry, Faculty of Science and Technology, Kinki University, Higashiosaka, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Hayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Nishimura
authors.forename: T
authors.initials: T
authors.lastname: Matsubara
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D006028:Glycosphingolipids / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D011161:Porifera / Q000032:analysis*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013055:Spectrophotometry, Infrared
chemical_list: D002241:Carbohydrates; D005227:Fatty Acids; D006028:Glycosphingolipids; C006948:ceramide digalactoside