title: Human hematopoietic stem cells in long-term culture: quantitation and manipulation.
abstract: These results suggest a potentially important role for the LTC system, not only in the development of a quantitative assay for hematopoietic cells, but also clinically as a procedure for manipulating autologous marrow in culture in a variety of ways for therapeutic advantage. These include strategies for immune modulation, gene marking and gene therapy, and perhaps eventually for stem cell amplification.
authors.affiliation: Leukemia/Bone Marrow Transplantation Program of British Columbia, Vancouver General Hospital.
authors.forename: M J
authors.initials: MJ
authors.lastname: Barnett
authors.forename: H J
authors.initials: HJ
authors.lastname: Sutherland
authors.forename: A C
authors.initials: AC
authors.lastname: Eaves
authors.forename: D E
authors.initials: DE
authors.lastname: Hogge
authors.forename: R K
authors.initials: RK
authors.lastname: Humphries
authors.forename: H G
authors.initials: HG
authors.lastname: Klingemann
authors.forename: P M
authors.initials: PM
authors.lastname: Lansdorp
authors.forename: G L
authors.initials: GL
authors.lastname: Phillips
authors.forename: D E
authors.initials: DE
authors.lastname: Reece
authors.forename: J D
authors.initials: JD
authors.lastname: Shepherd
mesh_terms: D016026:Bone Marrow Transplantation; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans