title: Prejunctional muscarinic receptors on cholinergic nerves in guinea pig airways are of the M2 subtype.
abstract: Prejunctional inhibitory muscarinic receptors in guinea pig tracheal strips were investigated by electrical field stimulation. Pilocarpine and methacholine caused, in a similar way, a dose-dependent increase in baseline with a concomitant decrease in twitch response. We showed by using selective muscarinic antagonists, such as pirenzepine (M1-selective), methoctramine (M2-selective), AF-DX 116 (11-[[2-[diethylamino)methyl]-1-piperidinyl]-acetyl]-5,11-dihydro- 6H-pyrido[2,3-b] [1,4]benzodiazepine-6-one, M2-selective), gallamine (M2-selective) and 4-DAMP (4-diphenylacetoxy-N- methylpiperidinemethiodide, M3-selective), that the prejunctional inhibitory muscarinic receptor is of the M2 subtype.
authors.affiliation: Department of Pharmacochemistry, Faculty of Chemistry, Vrije Universiteit, Amsterdam, The Netherlands.
authors.forename: C J
authors.initials: CJ
authors.lastname: Doelman
authors.forename: R C
authors.initials: RC
authors.lastname: Sprong
authors.forename: J E
authors.initials: JE
authors.lastname: Nagtegaal
authors.forename: J F
authors.initials: JF
authors.lastname: Rodrigues de Miranda
authors.forename: A
authors.initials: A
authors.lastname: Bast
mesh_terms: D000818:Animals; D003959:Diamines / Q000494:pharmacology; D004558:Electric Stimulation; D005703:Gallamine Triethiodide / Q000494:pharmacology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D010275:Parasympathetic Nervous System / Q000187:drug effects*; D010276:Parasympatholytics / Q000494:pharmacology; D010880:Piperidines / Q000494:pharmacology; D010890:Pirenzepine / Q000031:analogs & derivatives / Q000494:pharmacology; D011976:Receptors, Muscarinic / Q000187:drug effects*; D014132:Trachea / Q000187:drug effects / Q000502:physiology
chemical_list: D003959:Diamines; D010276:Parasympatholytics; D010880:Piperidines; D011976:Receptors, Muscarinic; D010890:Pirenzepine; C042375:4-diphenylacetoxy-1,1-dimethylpiperidinium; C054938:methoctramine; C048593:otenzepad; D005703:Gallamine Triethiodide