Titration of tannin via alkaline phosphatase activity.
An enzyme assay for tannin is described. It is based on the following steps: (i) bovine serum albumin (BSA) is absorbed onto polystyrene microplates; (ii) tannin is bound to the BSA-coated plates; (iii) alkaline phosphatase is then interacted with the free tannin-binding sites. The method takes advantage of the multiple hydroxyl groups of tannin which can associate more than one ligand, e.g., proteins. A pH-dependent dynamic equilibrium sets up between bound and unbound tannin and is controlled only by its initial concentration.
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Department of Food Science, Agricultural Research Organization, Israel.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008722:Methods; D012710:Serum Albumin, Bovine; D013634:Tannins / Q000032:analysis* / Q000378:metabolism
D013634:Tannins; D012710:Serum Albumin, Bovine; D000469:Alkaline Phosphatase