title: Development and evaluation of the polymerase chain reaction to detect Mycoplasma genitalium.
abstract: The polymerase chain reaction (PCR) was developed to detect Mycoplasma genitalium. Oligonucleotide primers were used to amplify a 374 bp region of the attachment protein of the mycoplasma. DNA from three strains of M. genitalium tested gave a characteristic PCR product which was not seen with DNA from any other source. As little as 10(-15) g of M. genitalium DNA could be detected and it was found in the vagina of progesterone-treated BALB/c mice inoculated with M. genitalium organisms later than they could be cultured from this site, but not in mice that never became colonised vaginally.
authors.affiliation: Division of Sexually Transmitted Diseases, Clinical Research Centre, Harrow, Middlesex, U.K.
authors.forename: H M
authors.initials: HM
authors.lastname: Palmer
authors.forename: C B
authors.initials: CB
authors.lastname: Gilroy
authors.forename: P M
authors.initials: PM
authors.lastname: Furr
authors.forename: D
authors.initials: D
authors.lastname: Taylor-Robinson
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000175:diagnosis* / Q000382:microbiology; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods*; D011374:Progesterone / Q000494:pharmacology; D014621:Vagina / Q000187:drug effects / Q000382:microbiology*; D014623:Vaginal Diseases / Q000175:diagnosis / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D015345:Oligonucleotide Probes; D011374:Progesterone