Detection of Chlamydia psittaci using DNA probes and the polymerase chain reaction.
Fewer than 10(5) elementary bodies of Chlamydia psittaci could be detected by using DNA hybridisation with a plasmid probe specific for avian chlamydial strains. PCR amplification of chlamydial DNA using primers specific for conserved regions of the major outer membrane protein gene enabled the detection of fewer than 10 elementary bodies. DNA could be amplified from 22 of the 24 chlamydial strains tested including avian, feline, ovine, caprine, koala and lymphogranuloma venereum strains.
Rasmussen
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Centre for Molecular Biotechnology, Queensland University of Technology, Brisbane, Australia.
Timms
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D000818:Animals; D002335:Carnivora; D002690:Chlamydia Infections / Q000175:diagnosis / Q000662:veterinary; D002691:Chlamydophila psittaci / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002522:Chlorocebus aethiops; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods*
D015342:DNA Probes; D004279:DNA, Viral; D015345:Oligonucleotide Probes