Immunogold fine structural localization of extracellular matrix components in aged human cornea. I. Types I-IV collagen and laminin.
Using the immunogold technique combined with cryoultramicrotomy and London Resin white (LR white) embedding, we studied the fine structural distribution of types I-IV collagen and laminin in corneal tissue from seven enucleated human eyes (age range, 63-78 years). Type II collagen was not identified in any corneal layer. Type I and type III collagen were distributed in a similar fashion in striated collagen fibrils in Bowman's layer and in the stroma. Type IV collagen was located only in the posterior non-banded region of Descemet's membrane. Laminin was identified in subepithelial anchoring plaques and the sub-endothelial region of Descemet's membrane in accordance with its recognized adhesive function.
Marshall
G E
GE
Department of Ophthalmology, University of Glasgow, United Kingdom.
Konstas
A G
AG
Lee
W R
WR
D000368:Aged; D003094:Collagen / Q000032:analysis* / Q000648:ultrastructure; D003315:Cornea / Q000737:chemistry* / Q000648:ultrastructure; D016326:Extracellular Matrix Proteins / Q000032:analysis* / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007797:Laminin / Q000032:analysis* / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged
D016326:Extracellular Matrix Proteins; D007797:Laminin; D003094:Collagen