title: Primary structure of a trisialylated oligosaccharide from human milk.
abstract: A novel oligosaccharide has been isolated from human milk and identified as a trisialylated derivative of lacto-N-hexaose. This compound was purified by combining ion-exchange chromatography, preparative paper chromatography and high performance liquid chromatography. Structural analyses by methylation, fast atom bombardment-mass spectrometry and proton nuclear magnetic resonance spectroscopy are consistent with the following structure: formula: see text.
authors.affiliation: Laboratoire de Chimie Biologique, Unité Mixte de Recherche du CNRS No 111, Villeneuve d'Ascq, France.
authors.forename: S
authors.initials: S
authors.lastname: Fievre
authors.forename: J M
authors.initials: JM
authors.lastname: Wieruszeski
authors.forename: J C
authors.initials: JC
authors.lastname: Michalski
authors.forename: J
authors.initials: J
authors.lastname: Lemoine
authors.forename: J
authors.initials: J
authors.lastname: Montreuil
authors.forename: G
authors.initials: G
authors.lastname: Strecker
mesh_terms: D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D002854:Chromatography, Paper; D006801:Humans; D008745:Methylation; D008895:Milk, Human / Q000737:chemistry*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D009844:Oligosaccharides; C016735:sialooligosaccharides