title: Protein synthesis requirements for nuclear division, cytokinesis, and cell separation in Saccharomyces cerevisiae.
abstract: Protein synthesis inhibitors have often been used to identify regulatory steps in cell division. We used cell division cycle mutants of the yeast Saccharomyces cerevisiae and two chemical inhibitors of translation to investigate the requirements for protein synthesis for completing landmark events after the G1 phase of the cell cycle. We show, using cdc2, cdc6, cdc7, cdc8, cdc17 (38 degrees C), and cdc21 (also named tmp1) mutants, that cells arrested in S phase complete DNA synthesis but cannot complete nuclear division if protein synthesis is inhibited. In contrast, we show, using cdc16, cdc17 (36 degrees C), cdc20, cdc23, and nocodazole treatment, that cells that arrest in the G2 stage complete nuclear division in the absence of protein synthesis. Protein synthesis is required late in the cell cycle to complete cytokinesis and cell separation. These studies show that there are requirements for protein synthesis in the cell cycle, after G1, that are restricted to two discrete intervals.
authors.affiliation: Department of Biology, University of Virginia, Charlottesville 22901.
authors.forename: D J
authors.initials: DJ
authors.lastname: Burke
authors.forename: D
authors.initials: D
authors.lastname: Church
mesh_terms: D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure*; D003513:Cycloheximide / Q000494:pharmacology*; D004261:DNA Replication / Q000187:drug effects*; D005656:Fungal Proteins / Q000096:biosynthesis*; D005838:Genotype; D016196:S Phase / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D014243:Trichodermin / Q000494:pharmacology*
chemical_list: D005656:Fungal Proteins; D014243:Trichodermin; D003513:Cycloheximide