Mechanisms implicated in the histamine response of the sheep ureterovesical junction.
The isotonic response of the sheep ureterovesical junction to histamine receptor agonists and antagonists was studied in vitro. Histamine (10(-7)-10(-4) M) produced a concentration-dependent contraction which was dependent on the extracellular calcium concentrations. Nifedipine (10(-9)-10(-6) M) partially inhibited the contractile response to histamine. 2-Methylhistamine and 2-pyridylethylamine elicited contractile effect in a concentration-related manner. The order of potency was histamine greater than 2-methylhistamine greater than 2-pyridylethylamine. Dimaprit and 4-methylhistamine produced no response in this tissue. The histamine contraction was antagonized by mepyramine, the pA2 value being 8.41, but not by cimetidine (10(-4) M). Scopolamine (10(-5) M) and indomethacin (10(-6) M) had an inhibitory effect. Phentolamine (10(-5) M), propranolol (10(-7)) and hexamethonium (10(-6) M) did not alter the histamine-induced contractions. The present results indicate that the response to histamine in sheep ureterovesical junction is mediated mainly by a direct action on the smooth muscle through excitatory histamine H1-receptors and partly by an indirect action via the stimulation of intramural cholinergic nerves.
Benedito
S
S
Department of Physiology, Faculty of Veterinary Sciences, Complutense University, Madrid, Spain.
Prieto
D
D
Rivera
L
L
Costa
G
G
García-Sacristán
A
A
D000818:Animals; D002118:Calcium / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006632:Histamine / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology; D011941:Receptors, Adrenergic / Q000187:drug effects / Q000502:physiology; D011950:Receptors, Cholinergic / Q000187:drug effects / Q000502:physiology; D011969:Receptors, Histamine H1 / Q000187:drug effects / Q000502:physiology; D011970:Receptors, Histamine H2 / Q000187:drug effects / Q000502:physiology; D012756:Sheep; D014513:Ureter / Q000187:drug effects* / Q000502:physiology; D001743:Urinary Bladder / Q000187:drug effects* / Q000502:physiology
D011941:Receptors, Adrenergic; D011950:Receptors, Cholinergic; D011969:Receptors, Histamine H1; D011970:Receptors, Histamine H2; D006632:Histamine; D002118:Calcium