Simultaneous use of standard and low-melting agarose for the separation and isolation of DNA by electrophoresis.
In this communication we describe the sequential use of standard and low-melting agarose in a single gel slab for the electrophoresis of DNA. This method has the advantages of high resolution and reproducibility characteristic of standard agarose and the ease of manipulation of DNA for direct cloning, sequential digestion and isolation, characteristic of low-melting agarose.
Fotadar
U
U
Department of Medicine, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, NY 10467.
Shapiro
L E
LE
Surks
M I
MI
D004247:DNA / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D012685:Sepharose; D013696:Temperature
D004247:DNA; D012685:Sepharose