[The effect of pure and long-stored commercial cholesterol on the binding of very low density and low density lipoproteins by rabbit hepatocytes (in vitro research)].
Effects of pure and long-stored commercial cholesterol feeding on rabbit plasma cholesterol level, on rabbit hepatocyte cholesterol esters levels, and on receptor activity of rabbit hepatocytes were investigated in three experimental groups. In comparison with control, the cholesterol levels in plasma of rabbits, fed with pure and long-stored cholesterol, were 3 and 15 times higher accordingly. Free cholesterol and cholesterol esters concentrations were enhanced in hepatocytes of rabbits fed with pure cholesterol (1.4 and 2.3 times, accordingly, p 0.05) and much more enhanced in hepatocytes of rabbits fed with long-stored cholesterol (4.5 and 24 times, accordingly, p less than 0.05). Specific binding of 125-1-labeled VLDL and LDL to rabbit hepatocytes was decreased in experimental groups by 20% and 32%, accordingly in the first group and by 40% and 77%, accordingly in the second group.
Volgushchev
S A
SA
Kosykh
V A
VA
Podrez
E A
EA
Novikova
D K
DK
Karpov
R S
RS
Repin
V S
VS
D000818:Animals; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism / Q000494:pharmacology*; D002788:Cholesterol Esters / Q000378:metabolism; D002791:Cholesterol, Dietary / Q000378:metabolism / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008079:Lipoproteins, VLDL / Q000187:drug effects* / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D011817:Rabbits
D002788:Cholesterol Esters; D002791:Cholesterol, Dietary; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D002784:Cholesterol
Vliianie chistogo i dolgo khranivshegosia kommercheskogo kholesterina na sviazyvanie lipoproteinov ochen' nizkoĭ i nizkoĭ plotnosti gepatotsitami krolikov (issledovaniia in vitro).