Resolution of Rhodocyclus gelatinosus photoreceptor unit components by temperature-induced phase separation in the presence of decyltetraoxyethylene.
A simple method for dissociating photoreceptor units from Rhodocyclus gelatinosus is described. Incubation of a chromatophore extract (Agalidis, I., Rivas, E. and Reiss-Husson, F. (1990) Photosynth. Res. 23, 249-255) at 4 degrees C with decyltetraoxyethylene and octyl-beta-D-thioglucopyranoside, followed by temperature-induced phase separation at 20 degrees C, led to the formation of three phases: a pellet composed of pure B875 antenna; an oily layer containing cytochrome c and other proteins; a detergent-poor supernatant containing crude reaction centers. This method provided a first step towards further purification of reaction centers and B875 antenna, respectively.
Agalidis
H
H
UPR 407, CNRS, Gif-sur-Yvette, France.
Reiss-Husson
F
F
D020130:Bacterial Chromatophores / Q000187:drug effects / Q000378:metabolism*; D004589:Electrophoresis, Disc; D007700:Kinetics; D046911:Macromolecular Substances; D008970:Molecular Weight; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology*; D012242:Rhodobacter sphaeroides / Q000378:metabolism*; D012244:Rhodospirillaceae / Q000378:metabolism*; D013055:Spectrophotometry, Infrared; D013816:Thermodynamics
D046911:Macromolecular Substances; D045322:Photosynthetic Reaction Center Complex Proteins; D011092:Polyethylene Glycols; C069485:decyltetraoxyethylene glycol monoether