Genetic and molecular characterization of an Adh-1 null mutant in tomato.
Treatment of tomato seeds with ethyl methanesulphonate (EMS) followed by allyl alcohol selection of M2 seeds has led to the identification of one plant (B15-1) heterozygous for an alcohol dehydrogenase (Adh) null mutation. Genetic analysis and expression studies indicated that the mutation corresponded to the structural gene of the Adh-1 locus on chromosome 4. Homozygous Adh-1 null mutants lacked ADH-1 activity in both pollen and seeds. Using an antiserum directed against ADH from Arabidopsis thaliana, which cross-reacts with ADH-1 and ADH-2 proteins from tomato, no ADH-1 protein was detected in seeds of the null mutant. Northern blot analysis showed that Adh-1 mRNA was synthesized at wild-type levels in immature seeds of the null mutant, but dropped to 25% in mature seeds. Expression of the Adh-2 gene on chromosome 6 was unaffected. The potential use of the Adh-1 null mutant in selecting rare transposon insertion mutations in a cross with "mutable" Adh-1+ tomato lines is discussed.
Wisman
E
E
Department of Molecular Biology, Agricultural University, Wageningen, The Netherlands.
Koornneef
M
M
Chase
T
T
Lifshytz
E
E
Ramanna
M S
MS
Zabel
P
P
D000426:Alcohol Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D000483:Alleles; D015152:Blotting, Northern; D003429:Cross Reactions; D004592:Electrophoresis, Starch Gel; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D017343:Genes, Plant; D006579:Heterozygote; D009154:Mutation; D010944:Plants / Q000235:genetics* / Q000276:immunology
D005020:Ethyl Methanesulfonate; D000426:Alcohol Dehydrogenase