Cloning and nucleotide sequence of a cDNA encoding ammodytoxin A, the most toxic phospholipase A2 from the venom of long-nosed viper (Vipera ammodytes).
A venom gland cDNA library was constructed in pUC9 and screened with a mixed oligonucleotide probe deduced from the unique Glu-4 to Ile-9 region of ammodytoxins. Twenty-one strongly positive clones were found by hybridization of about 5000 bacterial colonies, nine of them with the inserts encoding ammodytoxin A. The cDNA for ammodytoxin A encodes a 122 amino acid residue mature protein, preceded by a 16 residue signal peptide. Its complete nucleotide sequence shows 99% similarity to those of ammodytoxins B and C.
Pungercar
J
J
Department of Biochemistry, Jozef Stefan Institute, Ljubljana, Yugoslavia.
Kordis
D
D
Strukelj
B
B
Liang
N S
NS
Gubensek
F
F
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry*; D054501:Group II Phospholipases A2; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010741:Phospholipases A / Q000737:chemistry* / Q000235:genetics; D054467:Phospholipases A2; D015183:Restriction Mapping; D012911:Snakes; D014757:Viper Venoms / Q000737:chemistry* / Q000235:genetics
D015345:Oligonucleotide Probes; D014757:Viper Venoms; C065419:ammodytin L; D004247:DNA; C068117:ammodytoxin A; D010741:Phospholipases A; D054501:Group II Phospholipases A2; D054467:Phospholipases A2; C051125:ammodytoxin B; C060161:ammodytoxin C