Cytogenetic analysis of experimental interspecies goat-sheep chimera.
Chromosomal analysis was carried out on blood lymphocytes, skin fibroblasts, and germinal cells of an interspecies goat-sheep chimera. This chimera was produced by aggregation of blastomeres of goat and sheep embryos. A cell chimerism 54,XX/60,XY was found in blood lymphocytes and skin fibroblasts. At birth the percentage of lymphocytes with karyotype 54,XX (sheep) amounted to 80% and with karyotype 60,XY (goat) to 20%. With age the percentage of lymphocytes with chromosome complement 54,XX increased, so that at 18 months it was 94% sheep and 6% goat. At the same age, in skin fibroblasts the percentage of cells with goat karyotype reached 25%. Analysis of germinal cells showed in spermatogonia the presence of only karyotype 60,XY and in primary spermatocytes of 29 autosomal bivalents and the sex bivalent XY.
Jaszczak
K
K
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Mroków.
Członkowska
M
M
Guszkiewicz
A
A
Parada
R
R
D000375:Aging; D000818:Animals; D002478:Cells, Cultured; D002678:Chimera / Q000235:genetics*; D005347:Fibroblasts; D006041:Goats / Q000235:genetics*; D007621:Karyotyping; D008214:Lymphocytes; D008297:Male; D012756:Sheep / Q000235:genetics*; D013093:Spermatogonia; D013094:Spermatozoa