title: Molecular cloning of ompRS, a regulatory locus controlling production of outer membrane proteins in Erwinia carotovora subsp. carotovora.
abstract: A locus, ompRS, controlling synthesis of outer membrane proteins was cloned from Erwinia carotovora subsp. carotovora (Ecc) by complementation of an Escherichia coli ompR-envZ mutant. The Ecc ompRS locus was both structurally and functionally similar to the ompR-envZ operon controlling porin gene expression in E. coli as shown by DNA hybridization and complementation of E. coli ompR and envZ mutants. Furthermore, introduction of ompRS into E. coli delta (ompR-envZ) strains restored the osmoregulation of the major outer membrane protein genes ompC and ompF. Maxicell analysis of ompRS-carrying plasmids suggested that proteins similar in size to the E. coli ompR and envZ gene products were encoded by the Ecc ompR and ompS genes, respectively. Introduction of an ompRS transposon mutant onto the Ecc chromosome by marker exchange mutagenesis showed that ompRS is essential for production of a major outer membrane porin in Ecc. This mutational defect could be complemented by clones carrying Ecc ompRS or E. coli ompR envZ. The lack of the porin did not, however, compromise the virulence of these Ecc mutants.
authors.affiliation: Department of Molecular Genetics, Uppsala Genetic Center, Swedish University of Agricultural Sciences.
authors.forename: M B
authors.initials: MB
authors.lastname: Karlsson
authors.forename: M
authors.initials: M
authors.lastname: Pirhonen
authors.forename: H T
authors.initials: HT
authors.lastname: Saarilahti
authors.forename: E T
authors.initials: ET
authors.lastname: Palva
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004885:Erwinia / Q000235:genetics*; D004926:Escherichia coli; D005798:Genes, Bacterial; D005809:Genes, Regulator; D005814:Genes, Viral; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D015183:Restriction Mapping
chemical_list: D001425:Bacterial Outer Membrane Proteins