Purification and characterisation of a major allergen of Alternaria alternata.
A major allergen of Alternaria (Alt a Bd29K) was identified by SDS-PAGE and immunoblotting. It was purified by gel-permeation and ion-exchange HPLC. The apparent molecular mass was 29,000, with a cysteine linked sub-unit of 15,000 Mr. The apparent isoelectric point was 4.2. It was found to be a constituent of the Alt I allergen group.
Deards
M J
MJ
Bencard Allergy Unit, Beecham Pharmaceuticals, Epsom, Surrey, U.K.
Montague
A E
AE
D000485:Allergens / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D000528:Alternaria / Q000276:immunology*; D000596:Amino Acids / Q000032:analysis; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007073:Immunoglobulin E / Q000276:immunology; D007526:Isoelectric Point; D003904:Mitosporic Fungi / Q000276:immunology*; D008970:Molecular Weight; D010084:Oxidation-Reduction
D000485:Allergens; D000596:Amino Acids; D007073:Immunoglobulin E