Types and localization of aminopeptidases in different human blood cells.
1. Erythrocytes, polymorphonuclears, monocytes and lymphocytes isolated from human peripheral blood, were shown to possess in their cytosols, granules and microsomal fractions, aminopeptidases capable of hydrolysing arginyl-, leucyl-, methionyl-, phenylalanyl- and alanyl-2-naphthylamide. 2. In different cell compartments enzymes of different pI were responsible for these activities. 3. Chloride activated arginine aminopeptidase, broad specificity aminopeptidase and dipeptidyl peptidase III were found in cytosols of all examined cells. 4. In granules at least two aminopeptidases, a basic or neutral one, and an acidic one inactive at pH 4.4, could be discerned, whereas in microsomal fractions a broad specificity aminopeptidase preferring methionine was detected. 5. There is a considerable degree of similarity in the pattern of aminopeptidases within different blood cells. This may suggest that their functions are correlated to the physiological role of a particular cell compartment, rather than to that of a distinct cell type.
Grdisa
M
M
Department of Organic Chemistry and Biochemistry, University of Zagreb, Yugoslavia.
Vitale
L
L
D000626:Aminopeptidases / Q000097:blood*; D002712:Chlorides / Q000494:pharmacology; D003594:Cytoplasmic Granules / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000097:blood; D004912:Erythrocytes / Q000201:enzymology* / Q000648:ultrastructure; D006801:Humans; D007525:Isoelectric Focusing; D007962:Leukocytes / Q000201:enzymology* / Q000648:ultrastructure; D008214:Lymphocytes / Q000201:enzymology / Q000648:ultrastructure; D008861:Microsomes / Q000201:enzymology; D009000:Monocytes / Q000201:enzymology / Q000648:ultrastructure; D009504:Neutrophils / Q000201:enzymology / Q000648:ultrastructure; D013379:Substrate Specificity
D002712:Chlorides; D000626:Aminopeptidases; C019721:aminopeptidase B; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; C100152:dipeptidyl peptidase III