The radiosensitivity of populations of murine hemopoietic colony-forming cells that respond to combinations of growth factors.
Combinations of murine recombinant interleukin 3 (IL-3), purified murine macrophage colony-stimulating factor (M-CSF), and human recombinant interleukin 1 alpha (IL-1 alpha) were used to determine the effects of growth factors on the measured radiosensitivity of different populations of murine colony-forming cells (CFC). The data showed that combinations of growth factors resulted in different values of CFC radiosensitivity, being less than values observed when colony growth was stimulated using a single factor. For various combinations of growth factors, Do values ranged from 106 +/- 8 to 175 +/- 24 cGy for progenitor cells in normal bone marrow; 74 +/- 3 to 171 +/- 18 cGy for primitive multipotent CFC enriched using fluorescence-activated cell sorting; and from 46 +/- 4 to 131 +/- 10 cGy for more mature granulocyte-macrophage CFC, enriched by counterflow centrifugal elutriation. Only combinations of three factors produced the high values of Do reported in experiments using unpurified conditioned medium as a stimulus for colony formation.
Baird
M C
MC
Cancer Research Campaign Department of Radiobiology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, UK.
Hendry
J H
JH
Dexter
M T
MT
Testa
N G
NG
D000818:Animals; D001853:Bone Marrow / Q000502:physiology; D004338:Drug Combinations; D005260:Female; D005434:Flow Cytometry; D007377:Interleukin-3 / Q000008:administration & dosage / Q000494:pharmacology*; D016173:Macrophage Colony-Stimulating Factor / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051379:Mice; D011836:Radiation Tolerance; D013234:Stem Cells / Q000187:drug effects / Q000502:physiology*
D004338:Drug Combinations; D007377:Interleukin-3; D016173:Macrophage Colony-Stimulating Factor