Binding of cytosolic protein from cultured rat hepatocytes to the 3'-end of phosphoenolpyruvate carboxykinase mRNA--significance for protein-mediated mRNA stabilization.
In vitro transcripts of the 3'-non-translated region of the phosphoenolpyruvate carboxykinase (PCK) gene both in "sense" and "antisense" orientation bound cytosolic protein from cultured rat hepatocytes as demonstrated by electrophoretic mobility shift assay. Binding of cytosolic protein was increased 3-fold and PCK mRNA was enhanced 10-fold by treatment of the hepatocytes with 10 nM glucagon. The similar time course of the glucagon-induced increase in protein binding to PCK mRNA 3'-end and in PCK mRNA suggests that protein binding might be involved in the stabilization of PCK mRNA.
Christ
B
B
Institut für Biochemie, Universität Göttingen, Germany.
Heise
T
T
Jungermann
K
K
D000818:Animals; D002478:Cells, Cultured; D003600:Cytosol / Q000502:physiology; D015246:Deoxyribonuclease EcoRI; D004591:Electrophoresis, Polyacrylamide Gel; D005934:Glucagon / Q000494:pharmacology; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000235:genetics*; D010957:Plasmids; D011485:Protein Binding; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic / Q000187:drug effects
D011506:Proteins; D012333:RNA, Messenger; D005934:Glucagon; D015246:Deoxyribonuclease EcoRI; D010729:Phosphoenolpyruvate Carboxykinase (GTP)