Primary structure of a trisialylated oligosaccharide from human milk.
A novel oligosaccharide has been isolated from human milk and identified as a trisialylated derivative of lacto-N-hexaose. This compound was purified by combining ion-exchange chromatography, preparative paper chromatography and high performance liquid chromatography. Structural analyses by methylation, fast atom bombardment-mass spectrometry and proton nuclear magnetic resonance spectroscopy are consistent with the following structure: formula: see text.
Fievre
S
S
Laboratoire de Chimie Biologique, Unité Mixte de Recherche du CNRS No 111, Villeneuve d'Ascq, France.
Wieruszeski
J M
JM
Michalski
J C
JC
Lemoine
J
J
Montreuil
J
J
Strecker
G
G
D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D002854:Chromatography, Paper; D006801:Humans; D008745:Methylation; D008895:Milk, Human / Q000737:chemistry*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D009844:Oligosaccharides; C016735:sialooligosaccharides