Development and evaluation of the polymerase chain reaction to detect Mycoplasma genitalium.
The polymerase chain reaction (PCR) was developed to detect Mycoplasma genitalium. Oligonucleotide primers were used to amplify a 374 bp region of the attachment protein of the mycoplasma. DNA from three strains of M. genitalium tested gave a characteristic PCR product which was not seen with DNA from any other source. As little as 10(-15) g of M. genitalium DNA could be detected and it was found in the vagina of progesterone-treated BALB/c mice inoculated with M. genitalium organisms later than they could be cultured from this site, but not in mice that never became colonised vaginally.
Palmer
H M
HM
Division of Sexually Transmitted Diseases, Clinical Research Centre, Harrow, Middlesex, U.K.
Gilroy
C B
CB
Furr
P M
PM
Taylor-Robinson
D
D
D000818:Animals; D001431:Bacteriological Techniques; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000175:diagnosis* / Q000382:microbiology; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods*; D011374:Progesterone / Q000494:pharmacology; D014621:Vagina / Q000187:drug effects / Q000382:microbiology*; D014623:Vaginal Diseases / Q000175:diagnosis / Q000382:microbiology
D004269:DNA, Bacterial; D015345:Oligonucleotide Probes; D011374:Progesterone