A putative RNA virus in Babesia bovis.
Babesia bovis is an intraerythrocytic protozoan that causes bovine babesiosis. Agarose gel electrophoresis of nucleic acids extracted from two isolates of B. bovis reveals, besides bulk DNA, an ethidium bromide-stainable band at about 5.5 kb. Further characterization of the latter with DNase I, RNase and mung bean nuclease suggested it to be a double-stranded RNA. Sonicated parasites were fractionated in a CsCl buoyant density gradient. A sample containing the 5.5-kb RNA was analysed under an electron microscope and a virus-like particle was observed.
Johnston
R C
RC
Department of Biotechnology, Universidade Federal do Rio Grande do Sul, Porto Alegre-RS, Brazil.
Farias
N A
NA
Gonzales
J C
JC
Dewes
H
H
Masuda
A
A
Termignoni
C
C
Amako
K
K
Ozaki
L S
LS
D000818:Animals; D001403:Babesia / Q000235:genetics / Q000382:microbiology*; D001404:Babesiosis / Q000235:genetics; D002417:Cattle; D002499:Centrifugation, Density Gradient; D003850:Deoxyribonuclease I; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*; D012259:Ribonuclease, Pancreatic
D012330:RNA, Double-Stranded; D012367:RNA, Viral; D003850:Deoxyribonuclease I; D012259:Ribonuclease, Pancreatic