Binding and mobility of anti-dinitrophenyl monoclonal antibodies on fluid-like, Langmuir-Blodgett phospholipid monolayers containing dinitrophenyl-conjugated phospholipids.
The association of a fluorescently labelled anti-dinitrophenyl monoclonal antibody (ANO2) with Langmuir-Blodgett monolayers composed of three different binary mixtures of phosphatidylcholine and dinitrophenyl-conjugated phosphatidylethanolamine has been characterized. Quantitative fluorescence microscopy measurements demonstrated that measurable amounts of antibodies bound to the monolayers only at high molar fractions of dinitrophenyl-conjugated lipid (greater than or equal to 5 mol%). Fluorescence pattern photobleaching recovery measurements showed that the apparent translational diffusion coefficients and mobile fractions of a fluorescent lipid were high for all monolayer compositions and that the antibody translational mobility was measurable but slow and depended on the two-dimensional antibody density. The results demonstrate that the ANO2-binding characteristics of Langmuir-Blodgett monolayers containing dinitrophenyl-conjugated phospholipids are substantially different from those of similar model systems but that the ANO2 antibodies, when bound, display similar diffusive behavior.
Timbs
M M
MM
Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290.
Poglitsch
C L
CL
Pisarchick
M L
ML
Sumner
M T
MT
Thompson
N L
NL
D000911:Antibodies, Monoclonal / Q000276:immunology; D001665:Binding Sites; D004136:Dinitrobenzenes / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D007166:Immunosuppressive Agents / Q000378:metabolism; D008051:Lipid Bilayers; D010100:Oxygen / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013499:Surface Properties; D014867:Water / Q000737:chemistry
C045618:2,4-dinitrophenyl-IgG; D000911:Antibodies, Monoclonal; D004136:Dinitrobenzenes; D007074:Immunoglobulin G; D007166:Immunosuppressive Agents; D008051:Lipid Bilayers; D014867:Water; D010100:Oxygen