The calcium-binding protein MRP-8 is produced by human pulmonary tumor cells.
A calcium-binding protein was isolated from serum-free culture medium of human squamous carcinoma cells (HS 24). N-Terminal sequencing of the protein yielded 30 amino acids which were identical to the N-terminus of cystic fibrosis antigen. Northern blot analysis with an oligonucleotide derived from the N-terminus resulted in the detection of a transcript of approximately 600 bases. Screening of a HS 24-cDNA library with the same oligonucleotide led to the isolation of a 381-bp-cDNA encoding a protein of 93 amino acids. The corresponding protein has been identified as the calcium-binding protein MRP-8 usually found in Macrophages.
Schäfer
T
T
Institut für Biochemie, Technischen Hochschule Darmstadt.
Sachse
G E
GE
Gassen
H G
HG
D000595:Amino Acid Sequence; D001483:Base Sequence; D001798:Blood Proteins / Q000096:biosynthesis; D002135:Calcium-Binding Proteins / Q000096:biosynthesis*; D040501:Calgranulin A; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D014407:Tumor Cells, Cultured / Q000378:metabolism
D001798:Blood Proteins; D002135:Calcium-Binding Proteins; D040501:Calgranulin A; C026422:cystic fibrosis serum factor