Structure of Sm25, an antigenic integral membrane glycoprotein of adult Schistosoma mansoni.
Sm25 is the principal antigen recognised by antibodies from mice protectively vaccinated with isolated tegumental membranes of adult Schistosoma mansoni. The full-length amino acid sequence of this protein has been deduced from the sequence of two cDNAs, one isolated by screening a cDNA library and the other, including the 5' end of the gene, amplified directly from adult worm RNA using the polymerase chain reaction. The predicted sequence represents a nascent polypeptide of Mr 21,500. Following cleavage of a predicted signal sequence, the Mr of the resulting polypeptide is 17,600. The polypeptide contains 2 potential sites for N-linked glycosylation and a hydrophobic domain at the C-terminus that could facilitate membrane association. Analysis of the mature gene product confirmed that Sm25 is an N-glycosylated integral membrane protein and that the Mr of the deglycosylated polypeptide is between 15,000 and 20,000.
Ali
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Division of Parasitology, National Institute for Medical Research, Mill Hill, London, U.K.
Jeffs
S A
SA
Meadows
H M
HM
Hollyer
T
T
Owen
C A
CA
Abath
F G
FG
Allen
R
R
Hackett
F
F
Smithers
S R
SR
Simpson
A J
AJ
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D000954:Antigens, Surface; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D015723:Gene Library; D015801:Helminth Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011233:Precipitin Tests; D012550:Schistosoma mansoni / Q000235:genetics / Q000276:immunology*
D000947:Antigens, Helminth; D000954:Antigens, Surface; D015801:Helminth Proteins; D008562:Membrane Glycoproteins; C068960:Sm25 antigen, Schistosoma mansoni