title: Resolution of Rhodocyclus gelatinosus photoreceptor unit components by temperature-induced phase separation in the presence of decyltetraoxyethylene.
abstract: A simple method for dissociating photoreceptor units from Rhodocyclus gelatinosus is described. Incubation of a chromatophore extract (Agalidis, I., Rivas, E. and Reiss-Husson, F. (1990) Photosynth. Res. 23, 249-255) at 4 degrees C with decyltetraoxyethylene and octyl-beta-D-thioglucopyranoside, followed by temperature-induced phase separation at 20 degrees C, led to the formation of three phases: a pellet composed of pure B875 antenna; an oily layer containing cytochrome c and other proteins; a detergent-poor supernatant containing crude reaction centers. This method provided a first step towards further purification of reaction centers and B875 antenna, respectively.
authors.affiliation: UPR 407, CNRS, Gif-sur-Yvette, France.
authors.forename: H
authors.initials: H
authors.lastname: Agalidis
authors.forename: F
authors.initials: F
authors.lastname: Reiss-Husson
mesh_terms: D020130:Bacterial Chromatophores / Q000187:drug effects / Q000378:metabolism*; D004589:Electrophoresis, Disc; D007700:Kinetics; D046911:Macromolecular Substances; D008970:Molecular Weight; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology*; D012242:Rhodobacter sphaeroides / Q000378:metabolism*; D012244:Rhodospirillaceae / Q000378:metabolism*; D013055:Spectrophotometry, Infrared; D013816:Thermodynamics
chemical_list: D046911:Macromolecular Substances; D045322:Photosynthetic Reaction Center Complex Proteins; D011092:Polyethylene Glycols; C069485:decyltetraoxyethylene glycol monoether