Solid-phase enzyme-immunoassay of anti-insulin antibodies: effect of labeling site in insulin and of labeled number of horseradish peroxidase on the assay sensitivity.
Three horseradish peroxidase (HRP)-labeled porcine insulins which have definite labeling site(s) were compared regarding sensitivity in a solid-phase enzyme-immunoassay (EIA) of anti-insulin antibodies. The standard curves obtained with LysB29-HRP-insulin and GlyA1-HRP-insulin were steeper than that with GlyA1,LysB29-diHRP-insulin for both polyclonal and monoclonal antibodies. Thus, the mono-HRP-labeled insulins can afford higher sensitivities in the EIA. The importance of the HRP-labeling site in insulin and of the number of labeled HRP was first demonstrated by using HRP-labeled insulins having definite labeling site(s).
Zaitsu
K
K
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Nakayama
M
M
Nanami
M
M
Ohkura
Y
Y
D000818:Animals; D006735:Horseradish Peroxidase; D007124:Immunoenzyme Techniques; D007328:Insulin / Q000032:analysis*; D007330:Insulin Antibodies / Q000032:analysis*; D013552:Swine
D007328:Insulin; D007330:Insulin Antibodies; D006735:Horseradish Peroxidase