AP17 and AP19, the mammalian small chains of the clathrin-associated protein complexes show homology to Yap17p, their putative homolog in yeast.
AP17 and AP19 are the smallest polypeptide chain components of AP-2 and AP-1, the clathrin-associated protein complexes found in coated structures of the plasma membrane and Golgi apparatus of mammalian cells. cDNA clones representing the entire coding sequence of AP17 and AP19 were isolated from rat and mouse brain cDNA libraries, respectively. Determination of their nucleotide sequence predicts proteins of 142 and 158 amino acids with Mr 17,018 and 18,733. A sequence comparison of rat brain AP17 with mouse brain AP19 demonstrates that the small chains are highly related. A computer search for other related proteins has uncovered in yeast a previously unknown gene whose DNA sequence encodes a protein homologous to the small chain of AP complexes. The yeast sequence predicts Yap17p, a protein with 147 amino acids and a Mr of 17,373 that is slightly more related to the mammalian AP17 chain than to its AP19 counterpart.
Kirchhausen
T
T
Department of Anatomy and Cellular Biology, Harvard Medical School, Boston, Massachusetts 02115.
Davis
A C
AC
Frucht
S
S
Greco
B O
BO
Payne
G S
GS
Tubb
B
B
D033961:Adaptor Protein Complex 1; D033962:Adaptor Protein Complex 2; D034002:Adaptor Protein Complex sigma Subunits; D033942:Adaptor Proteins, Vesicular Transport; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000502:physiology*; D002417:Cattle; D002966:Clathrin / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005656:Fungal Proteins / Q000235:genetics* / Q000302:isolation & purification; D046911:Macromolecular Substances; D008961:Models, Structural; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010750:Phosphoproteins / Q000235:genetics* / Q000302:isolation & purification; D051381:Rats; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D012689:Sequence Homology, Nucleic Acid
C499068:APS1 protein, S cerevisiae; C069142:APS2 protein, S cerevisiae; D033961:Adaptor Protein Complex 1; D033962:Adaptor Protein Complex 2; D034002:Adaptor Protein Complex sigma Subunits; D033942:Adaptor Proteins, Vesicular Transport; C510066:Ap2s1 protein, mouse; C510067:Ap2s1 protein, rat; D002966:Clathrin; D005656:Fungal Proteins; D046911:Macromolecular Substances; D015345:Oligonucleotide Probes; D010750:Phosphoproteins; D029701:Saccharomyces cerevisiae Proteins; C499071:adaptor protein complex 1, sigma 1 subunit; D004247:DNA