title: A side-entry cold holder for cryo-electron microscopy.
abstract: A side-entry cold holder for cryo-electron microscopy has been constructed which can be used with no loss of image resolution or contrast at 3.4 A resolution compared to the normal room-temperature holder. A description is presented of some of the ideas and tests which we have developed during its construction and are included in the design, together with a briefer outline of the remaining limitations.
authors.affiliation: Medical Research Council, Laboratory of Molecular Biology, Cambridge, UK.
authors.forename: R
authors.initials: R
authors.lastname: Henderson
authors.forename: C
authors.initials: C
authors.lastname: Raeburn
authors.forename: G
authors.initials: G
authors.lastname: Vigers
mesh_terms: D015925:Cryopreservation; D008854:Microscopy, Electron / Q000379:methods*