title: Thermal inactivation study of glutathione peroxidase and glutathione reductase activities in lenses of primates and non-primates.
abstract: Heat lability studies of glutathione peroxidase and glutathione reductase activities were conducted on rabbit, sheep, rat, human, galago, cat and rhesus monkey lens supernatants. These species represent five mammalian orders. Incubation periods were 10.0 minutes in duration, with temperatures ranging from 25-100 degrees C (depending on which enzyme was being investigated). Results obtained for glutathione peroxidase activity demonstrated nearly identical heat lability profiles for human and rhesus monkey lenses. Both species were extremely labile to heat, losing activity at 30 degrees C and becoming totally inactive at temperatures of 50 degrees C (rhesus monkey) and 55 degrees C (human). Their profiles were very dissimilar to those of the other five species investigated, providing evidence for the existence of an evolutionary break. Glutathione reductase activity was extremely stable under conditions of highly elevated temperature for all seven species investigated. The human lens enzyme, the most stable of the species, maintained nearly 100% of its original activity up to 65 degrees C. Lenticular glutathione reductase activity did not reach zero levels in any of the seven species until a temperature of at least 80 degrees C was attained.
authors.affiliation: University of Minnesota, Department of Ophthalmology, Minneapolis 55455.
authors.forename: A M
authors.initials: AM
authors.lastname: Holleschau
authors.forename: W B
authors.initials: WB
authors.lastname: Rathbun
mesh_terms: D000818:Animals; D002415:Cats; D004795:Enzyme Stability; D005701:Galago; D005979:Glutathione Peroxidase / Q000378:metabolism*; D005980:Glutathione Reductase / Q000378:metabolism*; D006358:Hot Temperature; D006801:Humans; D007908:Lens, Crystalline / Q000201:enzymology*; D008253:Macaca mulatta; D011817:Rabbits; D051381:Rats; D012756:Sheep
chemical_list: D005979:Glutathione Peroxidase; D005980:Glutathione Reductase