Odontoblast-like cytodifferentiation of human dental pulp cells in vitro in the presence of a calcium hydroxide-containing cement.
The cement produced microcrystals of calcite by reaction with culture medium supplemented with calf serum. Human dental pulp cells seeded on such a substrate preferentially adhered and aggregated around the microcrystals. Immunofluorescence and immunogold labelling revealed a high affinity of serum fibronectin molecules for the calcite crystals. At 4 weeks in culture, the cells had various features of differentiated odontoblasts, notably nuclear polarization, typical appearance of the Golgi apparatus, synthesis of type I collagen and absence of type III, and apical accumulation of actin and vimentin. These cells also elaborated a collagenous extracellular matrix which did not mineralize.
Seux
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Laboratoire d'Histophysiologie et de Pathologie des Tissus Dentaires, UA CNRS 244, Faculté d'Odontologie, Lyon, France.
Couble
M L
ML
Hartmann
D J
DJ
Gauthier
J P
JP
Magloire
H
H
D000293:Adolescent; D002126:Calcium Hydroxide / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002648:Child; D003094:Collagen / Q000032:analysis; D003461:Crystallography; D003738:Dental Cements / Q000494:pharmacology*; D003782:Dental Pulp / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects; D004577:Electron Probe Microanalysis; D005353:Fibronectins / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008903:Minerals / Q000494:pharmacology; D009804:Odontoblasts / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects; D014404:Tubulin / Q000032:analysis; D014746:Vimentin / Q000032:analysis; D014961:X-Ray Diffraction
D003738:Dental Cements; D005353:Fibronectins; D008903:Minerals; D014404:Tubulin; D014746:Vimentin; C025702:Dycal; D003094:Collagen; D002126:Calcium Hydroxide