Procedure for selecting monoclonal antibodies for use in a ligand displacement assay of serum antibody levels.
Monoclonal antibodies for use in a ligand displacement assay were selected for specificity and affinity/avidity properties that result in their release and displacement in the presence of specific sample antibody but not in the presence of antibodies against other antigens. A screening process is described which involves measurement of displacement of antibody by an ELISA procedure using an enzyme labeled anti-immunoglobulin, providing a means of demonstrating usefulness of a candidate antibody in a ligand displacement format without necessitating the production of enzyme conjugates of each candidate antibody to be screened. The procedure was used to screen a set of eleven monoclonal antibodies (initially selected for anti-Trichinella spiralis specificity by conventional screening methods), and successfully discriminated between antibodies which were useful in the ligand displacement format and those which were not.
Middlebrooks
B L
BL
Department of Biological Sciences, University of Southern Mississippi, Hattiesburg.
Voss
P G
PG
Douglas
W L
WL
Toom
P M
PM
D000818:Animals; D000906:Antibodies / Q000097:blood*; D000909:Antibodies, Helminth / Q000097:blood; D000911:Antibodies, Monoclonal; D000915:Antibody Affinity; D000918:Antibody Specificity; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000276:immunology; D007118:Immunoassay / Q000379:methods*; D008024:Ligands; D051379:Mice; D011817:Rabbits; D014234:Trichinella / Q000276:immunology; D014235:Trichinellosis / Q000276:immunology
D000906:Antibodies; D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D008024:Ligands