title: Functional subpopulations of individual pancreatic B-cells in culture.
abstract: In the present study we have used a reverse hemolytic plaque assay and frequency distribution analysis to characterize the insulin secretory response of individual rat pancreatic B-cells in culture. Heterogeneous secretory behavior was observed with a multimodal distribution, suggesting the existence of B-cell subpopulations. Insulin secretion in response to increased extracellular glucose involved both amplification of secretion by individual cells as well as recruitment of previously silent B-cells.
authors.affiliation: Department of Neurosciences, Universidad Nacional Autonoma de Mexico, Mexico City.
authors.forename: M
authors.initials: M
authors.lastname: Hiriart
authors.forename: M C
authors.initials: MC
authors.lastname: Ramirez-Medeles
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D003470:Culture Media; D005947:Glucose; D006462:Hemolytic Plaque Technique; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D009994:Osmolar Concentration
chemical_list: D003470:Culture Media; D007328:Insulin; D005947:Glucose