Cyclosporine determinations in heart and liver transplant recipients: comparison of HPLC and FPIA methods on whole blood and plasma.
One hundred and eighty five whole blood samples were analysed for cyclosporine levels by fluorescence polarization immunoassay (FPIA) and high performance liquid chromatography (HPLC). 123 came from 4 heart transplant recipients (mean age +/- SD: 47.50 +/- 20.56 years) and 62 from 4 liver transplant recipients (44.50 +/- 16.52 years). FPIA was done on plasma and whole blood in heart transplant recipients, on plasma in the liver recipients. HPLC was always done on whole blood. The results show a good correlation between FPIA on plasma (y) and HPLC (x) in liver recipients (n = 62, r = 0.935, y = 1.23x + 70 ng/ml), slightly worse between FPIA on plasma (y) and HPLC (x) in heart recipients (n = 64, r = 0.610, y = 0.78x + 189 ng/ml) and mediocre for FPIA on whole blood (y) and HPLC (x) in heart recipients (n = 123, r = 0.566, y = 1.35x + 594 ng/ml).
Corda
C
C
Service de Pharmacologie Clinique et de Toxicologie du C.H.U. de Dijon,
Escousse
A
A
Santona
L
L
Guédon
F
F
Mounié
J
J
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D003524:Cyclosporins / Q000097:blood*; D005260:Female; D016231:Fluorescence Polarization Immunoassay; D016027:Heart Transplantation; D006801:Humans; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D011184:Postoperative Period
D003524:Cyclosporins