Cell cycle arrest and cellular differentiation mediated by a cell surface sialoglycopeptide.
Cell cycling by a relatively wide variety of cell lines was shown to be reversibly inhibited by a cell surface sialoglycopeptide (SGP) isolated and purified from intact bovine cerebral cortex cells. Cell cycle arrest, mediated by the bovine SGP inhibitor, was shown to be completely reversible with mouse Swiss 3T3, mouse 1316 fibrosarcoma, mouse N2a neuroblastoma, bovine MDBK and monkey BSC-1 cells. These cell lines represented both fibroblast and epithelial-like cells, transformed and nontransformed cells, as well as their being derived from a broad array of species. In contrast to the others tested, human HL-60 leukemic cells were sensitive to the inhibitory effects of the SGP but did not reenter the mitotic cycle after the removal of the inhibitor. Instead, the mitotic arrest of HL-60 cells appeared to enhance entry into a terminal and irreversible state of cellular differentiation.
Edson
G D
GD
Center for Basic Center Research and BioServe Space Technologies, Kansas State University, Manhattan 66506.
Fattaey
H K
HK
Johnson
T C
TC
D000818:Animals; D002417:Cattle; D002453:Cell Cycle / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D004847:Epithelial Cells; D005347:Fibroblasts / Q000166:cytology; D005354:Fibrosarcoma / Q000473:pathology; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D007942:Leukemia, Experimental / Q000473:pathology; D007951:Leukemia, Myeloid / Q000473:pathology; D051379:Mice; D009447:Neuroblastoma / Q000473:pathology; D012795:Sialoglycoproteins / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D006131:Growth Inhibitors; D012795:Sialoglycoproteins; C047908:sialoglycopeptide inhibitor protein, bovine