Cloning and characterization of the Saccharopolyspora erythraea fdxA gene encoding ferredoxin.
The Saccharopolyspora erythraea gene (fdxA) corresponding to a previously purified ferredoxin [Shafiee and Hutchinson, J. Bacteriol., 170 (1988) 1548-1553] was cloned using an oligodeoxyribonucleotide probe based on the N-terminal sequence of the ferredoxin. The nucleotide sequence of a 1.3-kb segment encompassing fdxA indicates that the corresponding protein, SeFdI, is 105 amino acids long, and very similar to other 7Fe ferredoxins. A partial open reading frame closely linked to fdxA was also detected. Disruption of fdxA was attempted by replacing the wild-type allele with an in vitro mutated copy. The failure to construct an fdxA mutant strain suggests that fdxA lies in an essential region of the S. erythraea chromosome.
Donadio
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Corporate Molecular Biology, Abbott Laboratories, Abbott Park, IL 60064.
Hutchinson
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D000595:Amino Acid Sequence; D001420:Bacteria, Aerobic / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005288:Ferredoxins / Q000235:genetics*; D015723:Gene Library; D005798:Genes, Bacterial; D006094:Gram-Positive Bacteria / Q000235:genetics*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016366:Open Reading Frames; D011995:Recombination, Genetic; D015183:Restriction Mapping
D004269:DNA, Bacterial; D005288:Ferredoxins; D015345:Oligonucleotide Probes