Specific binding sites for growth hormone in cultured mouse thymic epithelial cells.
Growth Hormones bound specifically to murine Thymic epithelial cells, which represent the major component of thymic micro-environment and can be modulated by pituitary hormones. The Kds found with human growth hormone and bovine growth hormone were 0.14 and 0.27 nM with a Bmax 0.56 and 0.35 fmol/10(6) cells respectively. Competition experiment analysis showed ED50 of 0.24 nM for hGH, 0.46 nM for rGH, 0.71 nM for bGH, 11.8 nM for hPRL and 11.2 nM for oPRL. No specific binding of [125I]-oPRL was observed under the same conditions. Both hPRL and bGH showed a negative regulatory effect on the number of the hGH binding sites when incubated with the culture for three days. The presence of GH receptors on Thymic epithelial cells provides biochemical evidence for the effect of GH on thymic function.
Ban
E
E
Pharmacology N.I.E., Institut Pasteur, CNRS UA1113, Paris.
Gagnerault
M C
MC
Jammes
H
H
Postel-Vinay
M C
MC
Haour
F
F
Dardenne
M
M
D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D002460:Cell Line; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D013006:Growth Hormone / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D011388:Prolactin / Q000378:metabolism / Q000494:pharmacology; D011986:Receptors, Somatotropin / Q000378:metabolism*; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism*
D011986:Receptors, Somatotropin; D011388:Prolactin; D013006:Growth Hormone