Detection of methicillin-resistant Staphylococcus aureus (MRSA) using polymerase chain reaction amplification.
Polymerase chain reaction (PCR) was applied for the detection of methicillin-resistant Staphylococcus aureus (MRSA). This procedure amplified a segment of MRSA-PBP (penicillin-binding protein) gene of DNA extract from the clinical isolates of S. aureus. A 1339-base-pair fragment of MRSA-PBP gene in DNA from S. aureus (MIC of methicillin, greater than or equal to 16 micrograms/ml) was amplified and detected by a specific oligonucleotide probe. Moreover, a 4.3 kb HindIII fragment containing MRSA-PBP gene was detected by using the same oligonucleotide probe. On the other hand, no PCR-amplified product was detected in DNA from methicillin-sensitive S. aureus (MIC of methicillin, less than 16 micrograms/ml).
Tokue
Y
Y
Department of Internal Medicine, Tohoku University, Sendai.
Shoji
S
S
Satoh
K
K
Watanabe
A
A
Motomiya
M
M
D001483:Base Sequence; D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D008712:Methicillin / Q000494:pharmacology*; D016106:Methicillin Resistance; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*
D004269:DNA, Bacterial; D015345:Oligonucleotide Probes; D008712:Methicillin