Differential inhibition of rat hepatic glutathione S-transferase isoenzymes in the course of fascioliasis.
The effects of a subclinical fascioliasis at various stages of its development (at week 3, 6 and 9 after infection by oral administration of 20 metacercariae of Fasciola hepatica) in rats were determined on the activity of enzymes involved in liver metabolism of glutathione and on the subunit pattern of cytosolic glutathione S-transferase. The parasitic pathology was ascertained by clinical observation of the rats and at autopsy. Hepatic microsomal cytochrome P-450 content was significantly decreased in infected rats by week 3 and 6 post-infection. Not correlatively, the catalytic activities of glutathione S-transferase towards 1-chloro-2,4-dinitrobenzene and 1,2-dichloro-4-nitrobenzene were significantly lowered in last stages of the experimental fascioliasis (by week 6 and 9 post-infection). These decreases were correlated to that of subunit 1 as determined by means of high-performance liquid chromatography of cytosolic proteins whereas subunit 6 could also be decreased. Fascioliasis did not alter cytosolic glutathione, glutathione reductase and glutathione peroxidase activities or plasma glutathione S-transferase activity accepting 1-chloro-2,4-dinitrobenzene as the substrate.
Galtier
P
P
Laboratoire de Pharmacologie-Toxicologie, INRA, Toulouse, France.
Vandenberghe
Y
Y
Coecke
S
S
Eeckhoutte
C
C
Larrieu
G
G
Vercruysse
A
A
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005211:Fascioliasis / Q000201:enzymology*; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors*; D008099:Liver / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D005982:Glutathione Transferase