[Analysis of tumor DNA by flow cytometry on prostate cyto-aspiration product. Value of routine cytologic evaluation].
In this prospective study, flow cytometry DNA profile of 169 stage D2 prostatic carcinomas were compared with conventional cytologic data. Two transrectal fine-needle aspiration biopsies were performed in each patient. Aspirates were immediately fixed in 2% polyethylene glycol 1500* alcoholic solution (Merck). Suspensions were mixed and studied by a conventional cytologic technique (Papanicolaou) and by flow cytometry (propidium iodide stain on isolated nuclei). A highly significant correlation was found between the DNA profile and the cytologic grade (p less than 0.001). The DNA profile was bimodal in 14% (3/21) of aspirates containing benign or atypical cells, 18% (3/17) of grade I aspirates, 30% (13/43) of grade II aspirates and 71% (24/34) of grade III aspirates. Routine cytologic evaluation of cell suspensions evaluated by flow cytometry is important in clinical practice since both falsely unimodal and falsely bimodal profiles occur. Leukocytes or benign epithelial cells can interfere with the tumor cell population. In fine-needle aspirates, the broad range of non-malignant contaminating cells limits the value of immunocytochemistry and emphasizes the usefulness of routine conventional cytologic evaluation.
Piaton
E
E
Laboratoire de Cytologie, Hôpital Albert-Michallon, Grenoble, France.
Bringuier
P P
PP
Devonec
M
M
Seigneurin
D
D
Perrin
P
P
D000230:Adenocarcinoma / Q000453:epidemiology / Q000378:metabolism* / Q000556:secondary; D001707:Biopsy, Needle; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D011446:Prospective Studies; D011467:Prostate / Q000737:chemistry*; D011471:Prostatic Neoplasms / Q000453:epidemiology / Q000378:metabolism* / Q000556:secondary
D004273:DNA, Neoplasm
Analyse de l'ADN tumoral par cytométrie en flux sur produit de cyto-aspiration prostatique. Intérêt du contrôle cytologique de routine.