Nucleotide sequence of the unique nitrate/nitrite-inducible cytochrome P-450 cDNA from Fusarium oxysporum.
A cDNA clone for the nitrate/nitrite-inducible cytochrome P-450 (P-450) of the fungus Fusarium oxysporum (tentatively termed P-450dNIR) was isolated by an immunoscreening method. Sequence determination revealed a polypeptide of 403 amimo acid residues (Mr = 44,371), which was shown to contain the full-length sequence of the fungal P-450. The amino terminus region of the predicted sequence contained neither the signal-like, hydrophobic domain that is commonly observed in microsomal P-450s nor the tagging prosequence that is essential for localization of mitochondrial P-450s. Further, the sequence exhibited higher homologies against those of soluble bacterial P-450s, in particular P-450s of Streptomyces, rather than those of eukaryotic P-450s including yeast and fungal P-450s. These results are highly indicative that P-450dNIR is the first soluble P-450 derived from eukaryotic organisms. The unique features might be related to the novel function of P-450dNIR, which is involved in a dissimilatory reduction of nitrite by the fungus. P-450dNIR was classified into a new family, P-450LV, and the corresponding gene of the fungus was named CYP55.
Kizawa
H
H
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Tomura
D
D
Oda
M
M
Fukamizu
A
A
Hoshino
T
T
Gotoh
O
O
Yasui
T
T
Shoun
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D005670:Fusarium / Q000201:enzymology*; D006418:Heme / Q000378:metabolism; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism*; D015183:Restriction Mapping; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D009566:Nitrates; D009573:Nitrites; D006418:Heme; D004247:DNA; D003577:Cytochrome P-450 Enzyme System