A comparison of transcriptional regulatory element activities in transformed and non-transformed rat anterior pituitary cells.
Transformed (GH-3) and non-transformed (P3) rat anterior pituitary cells were compared in their ability to direct expression of plasmids containing a variety of eukaryotic transcriptional regulatory elements (TREs). These include the herpes simplex virus thymidine kinase (HSV-TK), Rous sarcoma virus long terminal repeat (RSV-LTR), simian virus 40 early (SV-40E), human cytomegalovirus immediate-early (CMV-IE) and mouse metallothionein 1 (mMT-1) TREs. Chloramphenicol acetyl transferase (CAT) gene expression served as a reporter in this study. Following transient transfection, the cell lines exhibited similar profiles of TRE utilization. In each cell line. CMV-IE was most efficient in directing reporter gene expression, although 2-fold greater activity was observed in GH-3 versus P3 cells. RSV-LTR directed gene expression was lower than that of CMV-IE while both HSV-TK and SV-40E were inactive in each cell line. Also, the mMT-1 promoter was inducible by addition of ZnCl2 to the culture media, though the level required for maximal activation differed between the two cell lines. Transfected GH-3 and P3 cells, therefore, displayed similar TRE utilization profiles yet significant differences were observed in the ability of these cell lines to respond to specific regulatory elements.
Coleman
T A
TA
Department of Zoological and Biomedical Sciences, Ohio University, Athens.
Chomczynski
P
P
Frohman
L A
LA
Kopchick
J J
JJ
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002478:Cells, Cultured; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005814:Genes, Viral; D013006:Growth Hormone / Q000378:metabolism; D010903:Pituitary Gland, Anterior / Q000166:cytology*; D010911:Pituitary Neoplasms / Q000473:pathology*; D010957:Plasmids; D011388:Prolactin / Q000378:metabolism; D011401:Promoter Regions, Genetic; D051381:Rats; D011919:Rats, Inbred Strains; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured / Q000378:metabolism
D011993:Recombinant Fusion Proteins; D011388:Prolactin; D013006:Growth Hormone