Properties of a protease-sensitive acceptor component in mouse brain synaptosomes for Clostridium botulinum type B neurotoxin.
To characterize an acceptor for Clostridium botulinum type B neurotoxin, its binding kinetics were examined with mouse brain synaptosomes treated with various enzymes. The amount of 125I-labelled neurotoxin bound to synaptosomes decreased upon treatment with lysyl endopeptidase, neuraminidase, or phospholipase C. The binding of the neurotoxin was partially recovered by incubation of neuraminidase-treated synaptosomes with ganglioside GT1b or GD1a. Gangliosides incorporated into untreated, lysyl endopeptidase-treated, and phospholipase C-treated synaptosomes had no effect on the binding of the neurotoxin. These results may suggest that type B neurotoxin binds to gangliosides in cooperation with a certain protease-sensitive substance on the neural membranes.
Ogasawara
J
J
Department of Veterinary Science, College of Agriculture, University of Osaka Prefecture, Japan.
Kamata
Y
Y
Sakaguchi
G
G
Kozaki
S
S
D000818:Animals; D001665:Binding Sites; D001905:Botulinum Toxins / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003014:Clostridium botulinum / Q000378:metabolism; D005732:Gangliosides / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D009439:Neuraminidase / Q000378:metabolism; D009498:Neurotoxins / Q000378:metabolism*; D011869:Radioligand Assay; D012697:Serine Endopeptidases / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism*; D010738:Type C Phospholipases / Q000378:metabolism
D005732:Gangliosides; D009498:Neurotoxins; D010738:Type C Phospholipases; D009439:Neuraminidase; D012697:Serine Endopeptidases; C036813:lysyl endopeptidase; D001905:Botulinum Toxins