A chromatographic and spectroscopic study of photodegraded metronidazole in aqueous solution.
Preparative reversed-phase HPLC has been used to isolate a yellow photodegradation (2) product from aqueous metronidazole (1) solutions buffered with citrate:phosphate. Compound 2 can be further degraded into colorless materials by light, heat, or the addition of the nonaqueous solvents. These products have been characterized by UV, IR, proton NMR, mass spectroscopy, and melting point determination. It is proposed that the initial yellow degradation product (2) is an "excimer ion-pair" formed by the stabilization of 1 in its first electronic excited state by the citrate molecule.
Godfrey
R
R
Cyanamid of Great Britain Limited, Divisoinal Laboratories, Gosport, Hampshire, U.K.
Edwards
R
R
D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D008795:Metronidazole / Q000737:chemistry*; D012996:Solutions; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D012996:Solutions; D008795:Metronidazole