title: Regulation of cysteine proteinases during different pathways of differentiation in cellular slime molds.
abstract: Cysteine proteinase activities have been determined using gelatin-SDS-PAGE analysis and assays based on peptide nitroanilides. Vegetative myxamoebae of all species examined contain high levels of cysteine proteinase activity present in multiple forms. In both Dictyostelium discoideum and Polysphondylium pallidum the proteinase content is dependent on whether the cells are grown axenically or in association with bacteria. In all instances development is accompanied by a decreased intracellular cysteine proteinase activity. This occurs during the formation of fruiting bodies in D. discoideum, microcysts in P. pallidum, and macrocysts in Dictyostelium mucoroides. Significant quantities of proteinase activity are always secreted by myxamoebae immediately on starvation. In D. mucoroides this leads to an almost total depletion of intracellular cysteine proteinases by the aggregation stage. As a consequence of this depletion it has been relatively easy to detect a developmentally regulated accumulation of cysteine proteinases at the enzyme activity level, something which has not yet proved possible with D. discoideum. Three cysteine proteinases are produced as D. mucoroides macrocysts develop and mature. In the case of microcyst formation in P. pallidum the proteinase contents of the developing cells and of the microcysts are dependent on how the myxamoebae are grown. In this developmental pathway at least, there is no absolute requirement for specific proteinases to be present (or absent) at a particular stage. The diversity of cysteine proteinases found in cellular slime molds and the variety of features apparent in their regulation suggest that they will prove to be very useful for investigating features of the structure/function relationships in this important group of enzymes.
authors.affiliation: Department of Biological and Molecular Sciences, School of Natural Sciences, University of Stirling, Scotland.
authors.forename: M J
authors.initials: MJ
authors.lastname: North
authors.forename: D A
authors.initials: DA
authors.lastname: Cotter
mesh_terms: D000595:Amino Acid Sequence; D003546:Cysteine Endopeptidases / Q000235:genetics* / Q000378:metabolism; D004023:Dictyostelium / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009235:Myxomycetes / Q000201:enzymology* / Q000254:growth & development / Q000502:physiology; D013172:Spores, Fungal
chemical_list: D003546:Cysteine Endopeptidases