Cloning and characterization of a novel sequence-specific DNA-binding protein recognizing the negative regulatory element (NRE) region of the HIV-1 long terminal repeat.
The present studies describe the isolation of a murine cDNA clone that encodes a novel DNA-binding protein recognizing the negative regulatory element (NRE) region of the HIV-1 long terminal repeat (LTR). This cDNA expresses a truncated protein with a functional DNA-binding domain, which is rich in glutamine/proline and serine/threonine, a characteristic of a majority of sequence-specific DNA-binding proteins and transcriptional factors. The cDNA hybridizes to a single-copy gene that is expressed as an approx. 4.2-kb mRNA in a variety of murine and human cell types, implying that this gene is expressed in an ubiquitous fashion. The NRE region has been reported to down-regulate LTR-directed gene expression [Rosen et al., Cell 41 (1985) 813-823]. This is the first sequence-specific DNA-binding protein reported to recognize the NRE region.
Calvert
I
I
Laboratory of Biochemical Physiology, Program Resources, Inc./DynCorp, National Cancer Institute-Frederick Cancer Research and Development Center, MD 21702-1201.
Peng
Z Q
ZQ
Kung
H F
HF
Raziuddin
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D051381:Rats; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D004268:DNA-Binding Proteins; D014157:Transcription Factors