Effect of fibronectin on corneal epithelial wound healing in the vitamin A-deficient rat.
The authors investigated the in vitro and in vivo effects of fibronectin (Fn) on the migration of corneal epithelium of vitamin A-deficient (A-) and pair-fed control (A+) rats. Groups treated with 50 micrograms/ml Fn showed accelerated healing of epithelium in vitro (P less than 0.05) compared with control groups of A- and A+ rats. However, when 100 micrograms/ml Fn eye drops were administered 14 times over 20 hr, they had no significant effect on A+ rats in vivo, but increased the healing in A- rats (P less than 0.05). In this model, Fn promoted the healing of corneal epithelium under A- conditions where decreased endogenous Fn is seen, whereas A+ corneas in vivo, which have sufficient Fn over the wound surface, did not benefit from topical Fn administration.
Watanabe
K
K
Cornea Unit, Eye Research Institute, Boston, MA 02114.
Frangieh
G
G
Reddy
C V
CV
Kenyon
K R
KR
D000287:Administration, Topical; D000418:Albumins / Q000494:pharmacology; D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D003315:Cornea / Q000187:drug effects* / Q000648:ultrastructure; D004032:Diet; D004848:Epithelium / Q000187:drug effects; D005353:Fibronectins / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D011919:Rats, Inbred Strains; D014802:Vitamin A Deficiency; D014945:Wound Healing / Q000187:drug effects*
D000418:Albumins; D005353:Fibronectins