Erythroid differentiation of K-562 cells induced by ethidium bromide.
The exposure of K-562 cells to ethidium bromide or acridine orange as DNA-intercalating agents induced their differentiation to hemoglobin-producing cells. The addition of L-ethionine or dimethylsulfoxide induced neither such differentiation nor changes in poly(A) polymerase activity of K-562 cells, though the addition of ethidium bromide, acridine orange, hydroxyurea, or 1-beta-arabinofuranosylcytosine induced erythroid differentiation and decreases in poly(A) polymerase activity. These results suggest that DNA-intercalation is one of the pathways triggering differentiation of K-562 cells and the decrease in poly(A) polymerase activity may be an important change associated with differentiation of K-562 cells.
Sasaki
R
R
Department of Hematology, Jichi Medical School, Tochigi, Japan.
Yoshida
H
H
Miura
Y
Y
Takaku
F
F
D000165:Acridine Orange / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D003561:Cytarabine / Q000494:pharmacology; D004257:DNA Polymerase II / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004996:Ethidium / Q000494:pharmacology*; D005001:Ethionine / Q000494:pharmacology; D006454:Hemoglobins / Q000096:biosynthesis; D006801:Humans; D007364:Intercalating Agents; D007700:Kinetics; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive; D011062:Polynucleotide Adenylyltransferase / Q000378:metabolism
D006454:Hemoglobins; D007364:Intercalating Agents; D003561:Cytarabine; D011062:Polynucleotide Adenylyltransferase; D004257:DNA Polymerase II; D004996:Ethidium; D000165:Acridine Orange; D005001:Ethionine; D004121:Dimethyl Sulfoxide