Screening for potassium channel modulators by a high through-put 86-rubidium efflux assay in a 96-well microtiter plate.
A rapid and sensitive 86Rb efflux assay to detect chemical compounds capable of modulating the ATP-dependent potassium (KATP) channel is described. This assay, which is performed in a 96-well microtiter plate, utilizes a substrate adherent cell line as the target, requires a small amount of 86Rb as the tracer, and is a suitable system for performing the biochemical and pharmacological characterization of the KATP-channel and its activators. Because this assay is amenable to automation, it presents a useful means for high-volume screening of chemical compounds on a routine basis.
Daniel
S
S
CNS Research Department, American Cyanamid Company, Pearl River, New York 10965.
Malkowitz
L
L
Wang
H C
HC
Beer
B
B
Blume
A J
AJ
Ziai
M R
MR
D000255:Adenosine Triphosphate / Q000494:pharmacology; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology; D002478:Cells, Cultured; D003981:Diazoxide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015221:Potassium Channels / Q000187:drug effects*; D012414:Rubidium Radioisotopes
D015221:Potassium Channels; D012414:Rubidium Radioisotopes; D000255:Adenosine Triphosphate; D015740:Calcitonin Gene-Related Peptide; D003981:Diazoxide