[The effect of nerve growth factor on cholinergic neurons in dissociated cultures of the septum pellucidum].
Neurons dissociated from septal area of fetal (E 18-19) rat brain were grown for 7 days in culture. Cholinergic neurons were identified by cytochemical demonstration of acetyl cholinesterase. Addition of the nerve growth factor to the culture medium (200 u/ml) increases the number of AChE-positive neurons, the size of the cell body and activity of AChE in these neurons.
Isaev
N K
NK
Viktorov
I V
IV
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D004622:Embryo, Mammalian; D006651:Histocytochemistry; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D011950:Receptors, Cholinergic / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D012688:Septum Pellucidum / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology
D009414:Nerve Growth Factors; D011950:Receptors, Cholinergic; D000110:Acetylcholinesterase
Vliianie faktora rosta nervov na kholinergicheskie neĭrony v dissotsiirovannykh kul'turakh prozrachnoĭ peregorodki (septum pellucidum).