Desmin as an immunochemical marker of human decidual cells and its expression in menstrual fluid.
The expression of intermediate filament proteins in human endometrial tissue was examined. Desmin was selectively expressed in decidualized stroma, as demonstrated by SDS-PAGE analysis and positive response with a monoclonal antibody specific for desmin in ELISA and in western blot analysis. The same monoclonal antibody specifically stained human decidual cells in decidualized endometrium (secretory endometrium) in formalin-fixed paraffin-embedded sections prepared from diagnostic curettage samples. Desmin was also detected in menstrual fluid. Therefore, desmin might serve as a biochemical and histochemical marker of human decidualized endometrium.
Halperin
R
R
Department of Obstetrics and Gynaecology, Sackler School of Medicine, Tel-Aviv University, Israel.
Fleminger
G
G
Kraicer
P F
PF
Hadas
E
E
D015415:Biomarkers; D015153:Blotting, Western; D001826:Body Fluids / Q000737:chemistry*; D003656:Decidua / Q000737:chemistry* / Q000166:cytology; D003893:Desmin / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008598:Menstruation / Q000378:metabolism*
D015415:Biomarkers; D003893:Desmin