title: [Clinical significance of creatine kinase isoform assay by a new reagent based on immunoinhibition method].
abstract: Clinical significance of creatine kinase (CK) isoform assay by a new diagnostic reagent based on immunoinhibition method was evaluated. In the method, a monoclonal antibody (CKM-G 01), which inhibits MM3 but not MM1 isoform, was used. Serial serum samples from 143 acute myocardial infarction (AMI) patients were examined in hospitals. Isoform ratio (I.R.: defined as follows; inhibited CK activity/uninhibited CK activity) increased most rapidly (whose peak appeared 8.5 h after onset of AMI), compared with total CK (18.3 h) and CK-MB (16.1 h). In 61 patient samples collected within 4 h from onset, I.R. in 25 samples (41%) exceeded reference interval, whereas total CK in only 17 samples (28%) did it. Reference interval of the I.R. was 0.42 +/- 0.33 (mean +/- SD) in 1,246 normal subjects. These results show that the assay of CK isoforms by the reagents based on immunoinhibition method is useful for earlier detection of AMI.
authors.affiliation: Research and Development Center, Unitika Ltd., Kyoto.
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Tomita
authors.forename: H
authors.initials: H
authors.lastname: Tsubota
authors.forename: Y
authors.initials: Y
authors.lastname: Uji
authors.forename: H
authors.initials: H
authors.lastname: Sugiuchi
authors.forename: H
authors.initials: H
authors.lastname: Okabe
authors.forename: Y
authors.initials: Y
authors.lastname: Takagi
authors.forename: K
authors.initials: K
authors.lastname: Gomi
authors.forename: K
authors.initials: K
authors.lastname: Yoneda
authors.forename: Y
authors.initials: Y
authors.lastname: Katayama
mesh_terms: D000911:Antibodies, Monoclonal; D004796:Clinical Enzyme Tests / Q000379:methods; D003402:Creatine Kinase / Q000097:blood*; D005260:Female; D006801:Humans; D007159:Immunologic Tests / Q000379:methods; D007202:Indicators and Reagents; D007527:Isoenzymes; D008297:Male; D009203:Myocardial Infarction / Q000175:diagnosis*
chemical_list: D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D007527:Isoenzymes; D003402:Creatine Kinase