title: Antibodies against synthetic amphipathic helical sequences of surfactant protein SP-B detect a conformational change in the native protein.
abstract: Synthetic peptides based on the native human sequence of surfactant protein B have been used to generate polyclonal monospecific antibodies against specific segments of the native SP-B protein. Circular dichroism analysis of the synthetic peptides shows they have a dominant helical content in structure promoting environments and tensiometric measurements indicate these peptides lower surface tension at air-water interfaces implying that they contain amphipathic alpha helical motifs. Antibodies directed against the C-terminal segment of SP-B react with the native protein in the oxidized and reduced state. Antibodies directed against the N-terminal sequence of SP-B react with the native protein only in the reduced state suggesting that this domain has a conformation dependent on disulfide bond formation.
authors.affiliation: Department of Pediatrics, King/Drew Medical Center-UCLA 90059.
authors.forename: B R
authors.initials: BR
authors.lastname: Fan
authors.forename: R
authors.initials: R
authors.lastname: Bruni
authors.forename: H W
authors.initials: HW
authors.lastname: Taeusch
authors.forename: R
authors.initials: R
authors.lastname: Findlay
authors.forename: A J
authors.initials: AJ
authors.lastname: Waring
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D011487:Protein Conformation; D011510:Proteolipids / Q000276:immunology*; D011663:Pulmonary Surfactants / Q000276:immunology* / Q000648:ultrastructure; D013329:Structure-Activity Relationship; D013499:Surface Properties
chemical_list: D010455:Peptides; D011510:Proteolipids; D011663:Pulmonary Surfactants