Mastoparan induces oscillations of cytosolic Ca2+ in rat pancreatic acinar cells.
Microfluorimetry of fura-2 was used to monitor [Ca2+]i in single cells stimulated with the G-protein activating agent mastoparan. Mastoparan induced the generation of [Ca2+]i oscillations, which in contrast to oscillations induced by low concentrations of CCK were acutely dependent on the presence of extracellular Ca2+. Oscillations were inhibited by phorbol ester. Sodium fluoride, a known activator of G-proteins, gave similar results. Both mastoparan and CCK induced turnover of inositol phosphates, at concentrations higher than necessary to induce oscillations.
Yule
D I
DI
Department of Physiology, University of Michigan Ann Arbor 48109-0622.
Williams
J A
JA
D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002766:Cholecystokinin / Q000494:pharmacology; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D007295:Inositol Phosphates / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins; D007700:Kinetics; D008297:Male; D008856:Microscopy, Fluorescence; D010179:Pancreas / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D012969:Sodium Fluoride / Q000494:pharmacology; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors; D014862:Wasp Venoms / Q000494:pharmacology*
D000470:Alkaloids; D007295:Inositol Phosphates; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D014862:Wasp Venoms; C037500:mastoparan; D012969:Sodium Fluoride; D002766:Cholecystokinin; D011493:Protein Kinase C; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium