title: Fast-atom-bombardment mass spectrometry of sulphated oligosaccharides from ovine lutropin.
abstract: The positive- and negative-ion f.a.b.-mass spectra and the fragmentation of sulphated oligosaccharides derived from ovine lutropin are described. Negative-ion f.a.b.-m.s. of methylated derivatives offers a sensitive and rapid method for screening glycans for sulphation, for defining the location of sulphated residues, and for sequencing sulphated branches. Positive-ion f.a.b.-m.s. gives complementary data on non-sulphated branches in both complex and hybrid-type sulphated structures.
authors.affiliation: Department of Biochemistry, Imperial College, London, Great Britain.
authors.forename: A
authors.initials: A
authors.lastname: Dell
authors.forename: H R
authors.initials: HR
authors.lastname: Morris
authors.forename: F
authors.initials: F
authors.lastname: Greer
authors.forename: J M
authors.initials: JM
authors.lastname: Redfern
authors.forename: M E
authors.initials: ME
authors.lastname: Rogers
authors.forename: G
authors.initials: G
authors.lastname: Weisshaar
authors.forename: J
authors.initials: J
authors.lastname: Hiyama
authors.forename: A G
authors.initials: AG
authors.lastname: Renwick
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D007986:Luteinizing Hormone / Q000737:chemistry*; D008745:Methylation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry; D012756:Sheep; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013431:Sulfates / Q000737:chemistry
chemical_list: D009844:Oligosaccharides; D013431:Sulfates; D007986:Luteinizing Hormone