Cloning of a phospholipase A2-activating protein.
Recently we have described the isolation and biochemical characterization of a phospholipase A2-activating protein (PLAP). We have cloned this protein and found it to be expressed as a 2.5-kilobase mRNA. The steady-state levels of PLAP mRNA are induced in smooth muscle and endothelial cells following treatment with leukotriene D4. The increased message levels coincide with increased amounts of PLAP. Synthetic antisense DNA was used to block the synthesis of PLAP and this treatment effectively blocked the activation of phospholipase A2 and the increased generation of prostanoids in smooth muscle and endothelial cells treated with leukotriene D4.
Clark
M A
MA
Smith Kline and French Laboratories, King of Prussia, PA 19479.
Ozgür
L E
LE
Conway
T M
TM
Dispoto
J
J
Crooke
S T
ST
Bomalaski
J S
JS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D016373:DNA, Antisense / Q000138:chemical synthesis; D004587:Electrophoresis, Agar Gel; D004789:Enzyme Activation; D004797:Enzyme-Linked Immunosorbent Assay; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits
D016373:DNA, Antisense; D011506:Proteins; D012333:RNA, Messenger; C055324:phospholipase A2-activating protein