title: Differential localization of TGF-beta 2 in mouse preimplantation and early postimplantation development.
abstract: The localization of transforming growth factor type beta 2 (TGF-beta 2) has been followed during preimplantation and early postimplantation murine development using an anti-peptide antibody that specifically recognizes TGF-beta 2. The staining pattern showed that TGF-beta 2 is expressed from the four-cell stage onward and is differentially regulated as cells diverge to various lineages. High levels of staining were found in the trophectoderm of the blastocyst but no staining was observed in the inner cell mass. During postimplantation development the primitive and embryonic ectoderm also lacked detectable staining while visceral endoderm stained well. Parietal endoderm cells also showed positive staining reaction although to a lesser extent than visceral endoderm cells. These findings were confirmed in model systems of the embryo, namely, embryonal carcinoma and embryonic stem cells differentiated to to cells with either visceral or parietal endoderm characteristics. The possible regulatory role of this factor in early embryogenesis is discussed.
authors.affiliation: Hubrecht Laboratory, The Netherlands Institute for Developmental Biology, Utrecht.
authors.forename: H G
authors.initials: HG
authors.lastname: Slager
authors.forename: K A
authors.initials: KA
authors.lastname: Lawson
authors.forename: A J
authors.initials: AJ
authors.lastname: van den Eijnden-van Raaij
authors.forename: S W
authors.initials: SW
authors.lastname: de Laat
authors.forename: C L
authors.initials: CL
authors.lastname: Mummery
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D046508:Culture Techniques; D004475:Ectoderm / Q000378:metabolism; D004707:Endoderm / Q000378:metabolism; D015870:Gene Expression; D051379:Mice; D008856:Microscopy, Fluorescence; D016212:Transforming Growth Factor beta / Q000096:biosynthesis*; D014327:Trophoblasts
chemical_list: D016212:Transforming Growth Factor beta