The effect of E coli virulence on bacterial translocation and systemic sepsis in the neonatal rabbit model.
In the surgical neonate, three factors that promote bacterial translocation and systemic infection are: (1) intestinal bacterial colonization and overgrowth; (2) compromised host defenses; and (3) disruption of the mucosal epithelial barrier. The newborn rabbit provides an excellent model to study these factors. Like the human, there is early closure of the gut mucosa to macromolecules, and nutrition can be maintained by breast or formula feeding. This study examines translocation and systemic sepsis after colonization with virulent K1 and avirulent K100 strains of Escherichia coli. New Zealand white rabbit pups (2 to 5 days old) were studied. The gastrointestinal tracts of 12 were colonized with K1 E coli; 14 were colonized with K100 E coli; 12 control animals were not inoculated. Mesenteric lymph node (MLN), liver, spleen, and colon homogenate were cultured 72 hours postinoculation. No bacteria were isolated from the colons of all but one control animal. Translocation or systemic sepsis did not occur. Translocation to the MLN was significantly increased (P less than .03) in K1 (50%) and K100 (36%) groups compared with controls (0%). Translocation to liver and spleen (systemic sepsis) was significantly increased (P less than .03) in K1 animals (67%) compared with K100 (0%) or controls (0%). Colonization by both strains of E coli led to translocation to the MLN, but only K1 E coli caused systemic sepsis. This suggests that although colonization by E coli in the newborn leads to translocation to the MLN, progression to systemic sepsis is the result of characteristics of the bacteria and/or neonatal host responses.
Jackson
R J
RJ
University of Pittsburgh School of Medicine, PA.
Smith
S D
SD
Wadowsky
R M
RM
DePudyt
L
L
Rowe
M I
MI
D000818:Animals; D000831:Animals, Newborn; D002465:Cell Movement; D003106:Colon / Q000382:microbiology; D004926:Escherichia coli / Q000302:isolation & purification / Q000472:pathogenicity*; D008099:Liver / Q000382:microbiology; D008198:Lymph Nodes / Q000382:microbiology; D008643:Mesentery / Q000382:microbiology; D011817:Rabbits; D018805:Sepsis / Q000382:microbiology*; D013154:Spleen / Q000382:microbiology