Sub-picosecond dynamics of excited state of primary electron donor in reaction centers of Rhodopseudomonas viridis as revealed by hole burning at 1.7K broad and narrow holes.
Within the QF band of the primary electron donor (P), the spectra of absorbance changes due to the formation of a state of P+QA- (QA is the primary quinone) at 1.7K in Rhodopseudomonas viridis reaction centers excited at 1014 nm have been shown to involve two spectral features characterized by: (i) a progression of broad (170-190 cm-1) Gaussian vibronic bands (S-factor = 1.4) separated by 150 cm-1 and (ii) a 'narrow' structure near 1014 nm, characterized by 0-0 transition at 1014 nm with a width of approximately 50 cm-1 and 0-1 transition at 1000 nm with the width of approximately 100 cm-1, and S-factor = 0.9. The width of 50 cm-1 can be related to either zero-phonon hole (ZPH) width or the structure involving phonon wings and ZPH being unresolved. Since dichroic value (approximately 0.37) is unvarying over the P band, the vibrations involved are totally symmetric. The ZPH (width of approximately 3 cm-1) and phonon wings (frequency of approximately 30 cm-1) are resolved within the P band near 1014 nm when the spectrum of delta A due to the formation of bacteriopheophytinL- is measured at 1.7K.
Ganago
A O
AO
Institute of Soil Science and Photosynthesis, USSR Academy of Sciences, Pushchino, Moscow region.
Shkuropatov AYa
Shuvalov
V A
VA
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004579:Electron Transport; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D012241:Rhodopseudomonas / Q000032:analysis*; D013053:Spectrophotometry
D045322:Photosynthetic Reaction Center Complex Proteins