Identification of the 14 kDa bile acid transport protein of rat ileal cytosol as gastrotropin.
The 14 kDa bile acid binding protein of rat ileal cytosol (I-BABP), previously shown to be the major intracellular transporter of bile acids in enterocytes, was purified by affinity chromatography and gel electrophoresis. Enzymatic digestion of I-BABP which had been electroblotted to nitrocellulose led to the recovery and sequence analysis of four peptides representing 47 residues of sequence (approximately 35% of the full sequence). All the peptide sequences displayed high levels of identity (greater than 60%) and homology (greater than 80%) to the sequences of porcine and canine gastrotropin. This high level of homology together with other features of I-BABP identify it as rat gastrotropin, establishing gastrotropin as the major intracellular bile acid carrier of rat enterocytes.
Vodenlich
A D
AD
Central Research Division, Pfizer Inc., Groton, CT 06340.
Gong
Y Z
YZ
Geoghegan
K F
KF
Lin
M C
MC
Lanzetti
A J
AJ
Wilson
F A
FA
D000595:Amino Acid Sequence; D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003600:Cytosol / Q000378:metabolism; D004285:Dogs; D050556:Fatty Acid-Binding Proteins; D005768:Gastrointestinal Hormones / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D006913:Hydroxysteroid Dehydrogenases; D007082:Ileum / Q000378:metabolism*; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000378:metabolism; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific)
D001647:Bile Acids and Salts; D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D005768:Gastrointestinal Hormones; D006913:Hydroxysteroid Dehydrogenases; D008562:Membrane Glycoproteins; C037643:bile acid binding proteins; C057275:fatty acid-binding protein 6; C435496:AKR1C2 protein, human; D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific)