An immunoreactive apoglycoprotein purified from Coccidioides immitis.
Deglycosylation of glycoproteins in a lysate of spherules of Coccidioides immitis has permitted purification and partial characterization of a proline-rich pronase-sensitive antigen. Moreover, soluble antigen specifically stimulated lymphocytes from persons with dermal delayed-type hypersensitivity to coccidioidal antigens. When related to reference coccidioidin by tandem two-dimensional immunoelectrophoresis, the antigen fused in the anodal region with a specific reference antigen (antigen 2). It did not show identity with coccidioidal antigens used in conventional serologic assays. Although immunoblots of the purified protein with monospecific rabbit antiserum showed a single antigen at 33 kDa, the parent spherule lysate bound the same antibody in a broad band between 70 and greater than 200 kDa, which could be explained by microheterogeneity of glycosylation. Immunoelectron microscopy using affinity-purified human antibodies localized the antigen to the cell wall and internal septa of spherules. These findings suggest that the apoglycoprotein may be important in human immune responses to coccidioidal infection.
Dugger
K O
KO
Medical and Research Services, Veterans Affairs Medical Center, Tucson 85723.
Galgiani
J N
JN
Ampel
N M
NM
Sun
S H
SH
Magee
D M
DM
Harrison
J
J
Law
J H
JH
D000946:Antigens, Fungal / Q000737:chemistry* / Q000302:isolation & purification; D001059:Apoproteins / Q000737:chemistry / Q000276:immunology; D015153:Blotting, Western; D003045:Coccidioides / Q000032:analysis*; D003046:Coccidioidin / Q000276:immunology; D006023:Glycoproteins / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D006858:Hydrofluoric Acid / Q000737:chemistry; D007526:Isoelectric Point; D008213:Lymphocyte Activation; D008970:Molecular Weight
D000946:Antigens, Fungal; D001059:Apoproteins; D006023:Glycoproteins; C009303:spherulin; D003046:Coccidioidin; D006858:Hydrofluoric Acid