title: Structure of Sm25, an antigenic integral membrane glycoprotein of adult Schistosoma mansoni.
abstract: Sm25 is the principal antigen recognised by antibodies from mice protectively vaccinated with isolated tegumental membranes of adult Schistosoma mansoni. The full-length amino acid sequence of this protein has been deduced from the sequence of two cDNAs, one isolated by screening a cDNA library and the other, including the 5' end of the gene, amplified directly from adult worm RNA using the polymerase chain reaction. The predicted sequence represents a nascent polypeptide of Mr 21,500. Following cleavage of a predicted signal sequence, the Mr of the resulting polypeptide is 17,600. The polypeptide contains 2 potential sites for N-linked glycosylation and a hydrophobic domain at the C-terminus that could facilitate membrane association. Analysis of the mature gene product confirmed that Sm25 is an N-glycosylated integral membrane protein and that the Mr of the deglycosylated polypeptide is between 15,000 and 20,000.
authors.affiliation: Division of Parasitology, National Institute for Medical Research, Mill Hill, London, U.K.
authors.forename: P O
authors.initials: PO
authors.lastname: Ali
authors.forename: S A
authors.initials: SA
authors.lastname: Jeffs
authors.forename: H M
authors.initials: HM
authors.lastname: Meadows
authors.forename: T
authors.initials: T
authors.lastname: Hollyer
authors.forename: C A
authors.initials: CA
authors.lastname: Owen
authors.forename: F G
authors.initials: FG
authors.lastname: Abath
authors.forename: R
authors.initials: R
authors.lastname: Allen
authors.forename: F
authors.initials: F
authors.lastname: Hackett
authors.forename: S R
authors.initials: SR
authors.lastname: Smithers
authors.forename: A J
authors.initials: AJ
authors.lastname: Simpson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D000954:Antigens, Surface; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D015723:Gene Library; D015801:Helminth Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011233:Precipitin Tests; D012550:Schistosoma mansoni / Q000235:genetics / Q000276:immunology*
chemical_list: D000947:Antigens, Helminth; D000954:Antigens, Surface; D015801:Helminth Proteins; D008562:Membrane Glycoproteins; C068960:Sm25 antigen, Schistosoma mansoni