Effects of nebracetam (WEB 1881 FU), a novel nootropic, as a M1-muscarinic agonist.
We here investigated the effects of nebracetam (WEB 1881 FU 4-aminomethyl-1-benzylpyrrolidin-2-one-hemifumarate), a novel pyrrolidinone nootropic, on the rise of intracellular Ca2+ concentration ([Ca2+]i) in Jurkat cells, a human leukemic T cell line. Nebracetam induced a rise of [Ca2+]i in the medium with 1 mM Ca2+ and without Ca2+ (plus 1 mM EGTA). The nebracetam-induced [Ca2+]i rise was blocked by atropine greater than pirenzepine greater than AF-DX 116. From these results, nebracetam seems to act as an agonist for human M1-muscarinic receptors.
Kitamura
Y
Y
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Kaneda
T
T
Nomura
Y
Y
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D006801:Humans; D010277:Parasympathomimetics; D010890:Pirenzepine / Q000031:analogs & derivatives / Q000494:pharmacology; D011619:Psychotropic Drugs / Q000494:pharmacology*; D011760:Pyrrolidinones / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014981:Xenopus
D010277:Parasympathomimetics; D011619:Psychotropic Drugs; D011760:Pyrrolidinones; D010890:Pirenzepine; D001285:Atropine; C048593:otenzepad; D002118:Calcium; C056640:nebracetam