Phosphatidylserine reverses the age-dependent decrease in cortical acetylcholine release: a microdialysis study.
In vivo basal acetylcholine (ACh) and choline (Ch) output from the parietal cortex of 3- and 19-month-old freely moving rats was measured by microdialysis. A dialysis tubing was inserted transversally through the parietal cortex 24 h before the experiment. ACh and Ch concentrations were determined in the same perfusate samples by HPLC with electrochemical detection. In 19-month-old rats treated with Tris buffer, ACh and Ch outputs were 39 and 16% lower, respectively, than in 3-month-old rats. Phosphatidylserine (PtdSer) administration (15 mg/kg i.p. daily) for 8 days to 19-month-old rats markedly attenuated the decrease in ACh release. The same treatment did not affect ACh and Ch outputs in 3-month-old rats. ACh and Ch outputs in 19-month-old rats administered either phosphatidylcholine (PtdCho) or o-phospho-dl-serine (P-Ser) (15 mg/kg i.p. daily) for 8 days were as low as in 19-month-old rats receiving Tris buffer only. It is possible that chronic PtdSer treatment improve ACh release in aging rats by increasing the availability of Ch for ACh synthesis.
Casamenti
F
F
Department of Preclinical and Clinical Pharmacology, University of Florence, Italy.
Scali
C
C
Pepeu
G
G
D000109:Acetylcholine / Q000378:metabolism*; D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000033:anatomy & histology; D001923:Brain Chemistry / Q000187:drug effects; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D002794:Choline / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003956:Dialysis; D008297:Male; D010275:Parasympathetic Nervous System / Q000187:drug effects; D010296:Parietal Lobe / Q000187:drug effects / Q000378:metabolism; D010718:Phosphatidylserines / Q000494:pharmacology*; D051381:Rats
D010718:Phosphatidylserines; D002794:Choline; D000109:Acetylcholine