title: Activity of human extravascular temocillin concentrations simulated in an in-vitro kinetic model against beta-lactamase producing gram-negative bacilli.
abstract: An in-vitro kinetic model was used to demonstrate the bactericidal activity of concentrations of temocillin achieved in extravascular body fluids following a 1 g intravenous dosage in man. Concentrations of temocillin attainable in peripheral lymph were bactericidal for ampicillin-resistant strains of Escherichia coli, Enterobacter cloacae and Klebsiella oxytoca. Likewise, concentrations of temocillin achieved in human blister fluid following a 12-hourly dosage regimen were bactericidal for cultures of Ent. cloacae and K. oxytoca which were resistant, respectively, to simulated blister fluid concentrations of cefotaxime and aztreonam. The bactericidal activity of extravascular concentrations of temocillin against these multiresistant, Gram-negative bacteria was a reflection of the beta-lactamase-stability and good pharmacokinetic properties of temocillin.
authors.affiliation: SmithKline Beecham Pharmaceuticals, Betchworth, Surrey, UK.
authors.forename: C E
authors.initials: CE
authors.lastname: Cooper
authors.forename: A R
authors.initials: AR
authors.lastname: White
authors.forename: B
authors.initials: B
authors.lastname: Slocombe
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D001826:Body Fluids / Q000378:metabolism*; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000201:enzymology; D006801:Humans; D008196:Lymph / Q000378:metabolism*; D008954:Models, Biological; D010406:Penicillins / Q000378:metabolism / Q000494:pharmacology*; D001618:beta-Lactamases / Q000096:biosynthesis
chemical_list: D000900:Anti-Bacterial Agents; D010406:Penicillins; C031367:temocillin; D001618:beta-Lactamases