A Pit-1 binding site 3' to the transcription start site inhibits transcription elongation in vitro.
The transcription factor Pit-1 can autoregulate its own expression by interacting with binding sites associated with the Pit-1 gene. One of these binding sites is located within the transcribed region of the gene and appears to be responsible for negatively regulating Pit-1 transcription. Using unique constructions in an in vitro transcription system, we show here that binding of Pit-1 to its recognition element can impede the progress of transcription elongation through the occupied site. We propose that this is a viable mechanism for Pit-1 autoregulation.
Smith
K P
KP
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284-7762.
Sharp
Z D
ZD
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D005786:Gene Expression Regulation; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D011388:Prolactin / Q000235:genetics; D011401:Promoter Regions, Genetic; D051381:Rats; D050817:Transcription Factor Pit-1; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; C494186:Pou1f1 protein, rat; D050817:Transcription Factor Pit-1; D014157:Transcription Factors; D011388:Prolactin; D004247:DNA