Fluorescence assay to monitor phagocytosis by bloodclot derived polymorphonuclear leucocytes. 2. Study of patients with psoriasis and the effect of exposing leucocytes to psoriatic skin scales.
Comparisons of phagocytic parameters were carried out by a recently developed fluorescence test which is reproducible, simple and fast. Phagocytosis by polymorphonuclear leucocytes (PMNs) obtained from patients with psoriasis was compared with that of healthy individuals. Psoriatic skin scales, non-sterile and sterile, were tested for stimulatory effect on PMNs and compared with the effect of normal skin scrapings. Results confirm enhanced phagocytosis of bacteria by PMNs from patients with psoriasis over that of PMNs from healthy volunteers. Furthermore, the supernatant fluid from suspensions of psoriatic skin scales, non-sterile and sterile, stimulated PMNs activity.
Musclow
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CE
Department of Pathology, Mount Sinai Hospital, Toronto, Canada.
Farkas-Himsley
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H
Kanee-Schachter
R
R
Rubino
K
K
Der
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S
D000328:Adult; D001777:Blood Coagulation; D005260:Female; D005453:Fluorescence; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D008297:Male; D009504:Neutrophils / Q000276:immunology*; D010587:Phagocytosis; D011565:Psoriasis / Q000276:immunology*; D012867:Skin / Q000276:immunology* / Q000382:microbiology; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000276:immunology; D013242:Sterilization
D005839:Gentamicins