title: Endogenous VH gene family expression in immunoglobulin-transgenic mice: evidence for selection of antibody repertoires.
abstract: VH gene family expression in single cells of the emergent, available and actual B cell repertoires of C57BL/6 mice was compared to that of two immunoglobulin (Ig)-transgenic B6 lines (B6-Sp6 and M54). We found that less than 5% of bone marrow cells of transgenic mice express endogenous VH genes and that the vast majority (95%) of the peripheral, mature B cell repertoire in these animals is composed of cells expressing the VHJ558 transgenic family. Unimmunized transgenic mice, however, diversify VH gene family usage by 'background' Ig-secreting cells in the spleen, greater than 50% of which express endogenous VH genes. The pattern of endogenous VH gene family expression in the actual repertoire of B6-Sp6 mice is indistinguishable from that of normal B6 mice. In contrast, actual repertoires of M54 mice differ by a 4- to 5-fold higher representation of the VHQ52 family. These results demonstrate a powerful positive selection of B cells into the secretory compartments of unimmunized animals, show that actual and available repertoires differ very markedly, and suggest that V region interactions participate in the selection of 'natural antibody' repertoires.
authors.affiliation: Unité d'Immunobiologie, Institut Pasteur, CNRS UA 359, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Grandien
authors.forename: A
authors.initials: A
authors.lastname: Coutinho
authors.forename: J
authors.initials: J
authors.lastname: Andersson
authors.forename: A A
authors.initials: AA
authors.lastname: Freitas
mesh_terms: D000818:Animals; D000916:Antibody Diversity; D001402:B-Lymphocytes / Q000276:immunology; D015870:Gene Expression; D005803:Genes, Immunoglobulin; D007113:Immunity, Innate; D051379:Mice; D008822:Mice, Transgenic; D005810:Multigene Family