Translocation of the alpha- and beta-isoforms of protein kinase C following activation of human T-lymphocytes.
We have analyzed how activation of human Jurkat T-cells by the mitogenic lectin, concanavalin A (Con A), may affect the cellular distribution of the alpha- and beta-isoforms of protein kinase C (PKC) in T-cells. In non-stimulated cells almost all of the alpha- and beta-PKC was localized to the cytoplasmic compartment. Stimulation with Con A caused a transient translocation of both alpha- and beta-PKC from the cytoplasm to the cell membrane. The alpha-isoform appeared to be translocated to a somewhat greater extent and for a longer period of time than the beta-form. Translocation was maximal between 1 and 5 min for both of the isoforms. 30 min after stimulation, beta-PKC had returned to basal levels, whereas a substantial amount of alpha-PKC remained associated with the particulate fraction. We conclude that activation of human T-cells causes the translocation of at least two different isoforms of PKC, alpha-PKC and beta-PKC.
Kvanta
A
A
Department of Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Jondal
M
M
Fredholm
B B
BB
D000595:Amino Acid Sequence; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D003208:Concanavalin A / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology; D006801:Humans; D007106:Immune Sera; D007118:Immunoassay; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000138:chemical synthesis; D011493:Protein Kinase C / Q000302:isolation & purification / Q000378:metabolism*; D013601:T-Lymphocytes / Q000201:enzymology / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007106:Immune Sera; D007527:Isoenzymes; D010455:Peptides; D003208:Concanavalin A; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate