Interpretation of results with the 8-azaguanine resistance system in Salmonella typhimurium: no evidence for direct acting mutagenesis by 15-oxosteviol, a possible metabolite of steviol.
15-Oxosteviol, postulated to be the mutagenic metabolite of steviol, was observed to be non-mutagenic in preliminary experiments using a number of different systems. Repetition of the original experiment in Salmonella TM677 failed to show any significant induction of 8-azaguanine resistant mutants by 15-oxosteviol even when the number of bacteria tested was greatly increased. Examination of the earlier positive result showed that it could not be justified from the data and revealed a commonly applied way of mishandling data obtained with the TM677 system.
Procinska
E
E
School of Molecular Sciences, University of Sussex, Falmer, Brighton, UK.
Bridges
B A
BA
Hanson
J R
JR
D001375:Azaguanine / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004224:Diterpenes / Q000378:metabolism / Q000633:toxicity*; D045786:Diterpenes, Kaurane; D004352:Drug Resistance, Microbial; D009152:Mutagenicity Tests / Q000379:methods; D009154:Mutation; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics
D004224:Diterpenes; D045786:Diterpenes, Kaurane; C069553:15-oxosteviol; C012042:steviol; D001375:Azaguanine; D004121:Dimethyl Sulfoxide