Effects of progesterone and gossypol on monoamine oxidase activity in human term placental explant.
Human placental explant was cultured, using fresh full term placenta delivered by elective cesarean section (n = 10), and monoamine oxidase (MAO) activity was measured in the tissue by the spectrophotometric method. Placentae were obtained from patients without complications in pregnancy. Chorionic villi 2 g was incubated for 3 hr under the 95% air-5% CO2 humidified condition at 37 degrees C. When progesterone was added in the media (100-500 micrograms/ml), MAO activities significantly decreased, compared with the control. The gossypol (100 micrograms/ml) treated group showed no inhibitory or stimulatory effects on MAO activities, compared with the control. However, progesterone (100-500 micrograms/ml) and gossypol (100 micrograms/ml) treated group showed the same value of MAO activities, compared with the control. Those results indicate that gossypol might block progesterone action which is inhibitory against MAO activity. The mechanism of pharmacological function of gossypol was discussed.
Kono
H
H
Department of Veterinary Physiology, Ohio State University, Columbus.
Lin
Y C
YC
Yamaguchi
M
M
Zuspan
F P
FP
Furuhashi
N
N
Takayama
K
K
Yajima
A
A
D005260:Female; D006072:Gossypol / Q000494:pharmacology*; D006801:Humans; D008995:Monoamine Oxidase / Q000378:metabolism*; D009924:Organ Culture Techniques; D010920:Placenta / Q000187:drug effects / Q000201:enzymology*; D011247:Pregnancy; D011374:Progesterone / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D011374:Progesterone; D008995:Monoamine Oxidase; D006072:Gossypol