DNA homology comparison between American and European Borrelia burgdorferi strains.
Seven strains of Borrelia burgdorferi isolated from ticks and from human beings in Europe and U.S.A. were analyzed for DNA restriction patterns with several enzymes and for DNA homology in Southern blot hybridizations. The restriction patterns showed a moderately high variability. In Southern blot hybridization, strain B31 (U.S.A.) DNA gave a strong signal with itself, strain Bsf (U.S.A.) and Alcaide (isolated in Italy but presumably contracted in Venezuela). Strain B45 (F.R.G.) hybridized to itself, strain BITS (Italy) and to strain D.A. (Italy). Strain Nancy (Italy) gave a signal only when hybridized to itself, although it was classified as Borrelia on the basis of the clinical manifestations, SDS-PAGE protein pattern and antigenic determinants. No hybridization differences were observed for strains isolated from different hosts in the same continental geographical area.
Fattorini
P
P
Istituto di Microbiologia, Università di Trieste, Italy.
Graziosi
G
G
Balanzin
D
D
Cinco
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D001096:Arachnid Vectors; D015373:Bacterial Typing Techniques; D015139:Blotting, Southern; D015748:Borrelia burgdorferi Group / Q000145:classification / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D005060:Europe; D005455:Fluorescent Antibody Technique; D015723:Gene Library; D014644:Genetic Variation / Q000235:genetics; D006801:Humans; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013987:Ticks / Q000382:microbiology; D014481:United States
D000911:Antibodies, Monoclonal; D004269:DNA, Bacterial