Cloning of chromosomal beta-lactamase genes from Yersinia enterocolitica.
Two beta-lactamase genes present in the chromosome of Yersinia enterocolitica have been cloned individually into the plasmid pACY184 and expressed in Escherichia coli. The gene for broad-spectrum beta-lactamase I ('A') was cloned from a strain belonging to the O:3 serotype, and the gene for (cephalosporinase) beta-lactamase II ('B') was cloned from a strain of the O:5b serotype. The properties of the beta-lactamases expressed in E. coli are similar to those previously described in Y. enterocolitica.
Seoane
A
A
Departamento de Biología Molecular, Universidad de Cantabria, Santander, Spain.
García Lobo
J M
JM
D002510:Cephalosporinase / Q000096:biosynthesis / Q000235:genetics*; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis; D015870:Gene Expression; D005798:Genes, Bacterial; D006868:Hydrolysis; D007700:Kinetics; D010405:Penicillinase / Q000096:biosynthesis / Q000235:genetics*; D015183:Restriction Mapping; D015008:Yersinia enterocolitica / Q000201:enzymology / Q000235:genetics*
D004269:DNA, Bacterial; D002510:Cephalosporinase; D010405:Penicillinase