Acute actions of 1,25-dihydroxyvitamin D3 upon chick pancreatic calbindin-D28K.
We have compared the relative responsiveness of pancreatic, intestinal and renal tissue calbindin-D28K protein content to the stimulatory actions of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] in vitamin D-deficient (-D) chicks. Tissue concentrations of calbindin-D28K were undetectable in the -D chick intestine but present, albeit at low concentrations (less than 1 microgram CaBP/mg protein) in the -D kidney and pancreas. Intestinal, pancreatic and renal calbindin-D28K content was stimulated 318, 9.8 and 2.9 fold respectively, 48 hours after -D chicks received a single dose of 1,25(OH)2D3 [6.5 nmol/animal]. The pancreatic calbindin-D28K content could be significantly stimulated as early as 5 hours after 1,25(OH)2D3 administrations in vivo. These findings support the contention that the pancreas is a target for vitamin D, and is consistent with the view that calbindin-D28K plays a role in normal pancreatic functions.
Hall
A K
AK
Division of Biomedical Sciences, University of California, Riverside 92521.
Norman
A W
AW
D000818:Animals; D064026:Calbindins; D002117:Calcitriol / Q000494:pharmacology*; D002118:Calcium / Q000097:blood; D002645:Chickens; D004305:Dose-Response Relationship, Drug; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D008297:Male; D009928:Organ Specificity; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism*; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D014808:Vitamin D Deficiency / Q000378:metabolism*
D064026:Calbindins; D064030:S100 Calcium Binding Protein G; D002117:Calcitriol; D002118:Calcium