title: Antibody reactivity to deletion mutants of the HIV-1 SF2 envelope.
abstract: In human immunodeficiency virus type 1 (HIV-1) infected individuals, the antibody response to the external envelope (gp120) is associated with in vitro neutralization. To further characterize the anti-gp120 response, we examined the IgG reactivity of 75 HIV-1-seroconverted and 200 HIV-1-seropositive individuals to deletion mutants of gp120 in an enzyme immunoassay. We used yeast-derived, non-glycosylated recombinant HIV-1 SF2 gp120 equivalent and-variants deleted in variable regions. We observed two distinctive response patterns: IgG non-responders (SF2-V3-restricted responders) and IgG responders to conserved regions of gp120. This divergence in response pattern occurred soon after gag/env HIV-1 antibody seroconversion and persisted in time within an individual. In addition, the SF2-V3-restricted responders had a higher frequency of HIV-1 core antigen positivity and HIV-1 core antibody negativity than the non-restricted responders. These results suggest that specific and persistent host antibody response patterns to gp120 develop early in HIV-1 infection and that these patterns are associated with differences in HIV-1 expression.
authors.affiliation: Department of Virology, Academic Medical Centre, Amsterdam, The Netherlands.
authors.forename: N K
authors.initials: NK
authors.lastname: Back
authors.forename: N L
authors.initials: NL
authors.lastname: Haigwood
authors.forename: F
authors.initials: F
authors.lastname: de Wolf
authors.forename: B M
authors.initials: BM
authors.lastname: de Jongh
authors.forename: J
authors.initials: J
authors.lastname: Goudsmit
mesh_terms: D015331:Cohort Studies; D015483:HIV Antibodies / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000276:immunology*; D006679:HIV Seropositivity / Q000276:immunology* / Q000382:microbiology; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006716:Homosexuality; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology*; D008137:Longitudinal Studies; D008297:Male; D009154:Mutation; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D007074:Immunoglobulin G; D010446:Peptide Fragments; D011994:Recombinant Proteins