title: Lysis of bovine platelets by Pasteurella haemolytica leukotoxin.
abstract: Pasteurella haemolytica A1 culture supernatants caused rapid cytolysis (less than 5 minutes) of isolated bovine platelets as measured by leakage of the cytoplasmic enzyme lactate dehydrogenase (LD). The platelet lytic factor had several features similar to P haemolytica leukotoxin. Like P haemolytica leukotoxin, the platelet lytic factor was produced by P haemolytica during logarithmic growth phase, was heat-labile, and was active against target cells (platelets) from ruminant species (cattle and sheep), but not from non-ruminant species (horses, pigs, and human beings). Additionally, the platelet lytic factor was neutralized with antileukotoxin rabbit serum. The amount of LD leaked by a fixed concentration of bovine platelets was proportional to the amount of toxin added at low toxic doses and became maximal at 88 +/- 11% of the total platelet LD activity for high doses of toxin. When a fixed dose of toxin was used and the platelet concentration was varied, LD leakage was initially proportional to the platelet concentration, but plateaued at higher platelet concentrations. The platelet lytic factor required Ca2+ and was inhibited by addition of the Ca2+ chelator ethylene glycol-bis(beta-aminoethyl ether)N,N,N',N'-tetraacetic acid. Toxin-mediated platelet damage may be important in thrombi formation and fibrin exudation typically associated with P haemolytica pleuropneumonia of cattle.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, Oklahoma State University, Stillwater 74078.
authors.forename: K D
authors.initials: KD
authors.lastname: Clinkenbeard
authors.forename: M L
authors.initials: ML
authors.lastname: Upton
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000201:enzymology; D002417:Cattle; D002478:Cells, Cultured; D005098:Exotoxins / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D010325:Pasteurella; D013045:Species Specificity
chemical_list: D001427:Bacterial Toxins; D005098:Exotoxins; C018579:leukotoxin; D007770:L-Lactate Dehydrogenase