The influence of diuretics on the excretion and metabolism of doping agents--V. Dimefline.
A sensitive method for the quantitative determination of the respiratory stimulant dimefline in 5 ml urine using capillary gas chromatography with nitrogen specific detection is presented. After the oral administration of a therapeutical amount of 16 mg dimefline to five subjects only 0.26 +/- 0.16% of the dose is excreted as the conjugated drug in 24 h. The maximum excretion rate occurred 3 h after dosing, the peak concentration being 154 +/- 60 ng ml-1. The influence of diuretics taken 2 h after the administration of dimefline was studied in three subjects. From these results it appeared that the use of acetazolamide and hydrochlorthiazide in order to circumvent a positive dimefline doping case is questionable. Due to the potent diuretic effect of furosemide, the intake of this diuretic could result in a suppression of the dimefline concentration below the detection limit of 10 ng ml-1.
Delbeke
F T
FT
Laboratorium voor Farmacologie en Toxicologie der Huisdieren, Faculteit Diergeneeskunde, Rijksuniversiteit Gent, Belgium.
Debackere
M
M
D000086:Acetazolamide / Q000494:pharmacology; D004232:Diuretics / Q000494:pharmacology*; D004300:Doping in Sports; D005419:Flavonoids / Q000652:urine*; D005665:Furosemide / Q000494:pharmacology; D006801:Humans; D006852:Hydrochlorothiazide / Q000494:pharmacology; D008297:Male
D004232:Diuretics; D005419:Flavonoids; D006852:Hydrochlorothiazide; D005665:Furosemide; C015154:dimefline; D000086:Acetazolamide