The effect of chemical radiation protectors on cell cycle progression after gamma or neutron irradiation.
The effects of two chemical radiation protectors, WR-1065 and WR-151326, were characterized in V79 Chinese hamster cells after either cobalt-60 (60Co) gamma or fission spectrum neutron irradiation. Each protector was administered at a concentration of 4 mM to exponentially growing cultures for 30 min prior to and during irradiation with either 60Co gamma or JANUS fission spectrum neutrons. After irradiation the cells were either plated immediately for survival or returned to the incubator and assayed for cell progression. Aliquots of cells were removed at selected times, counted, fixed and stained with 4'6-diamidino-2-phenylindole (DAPI). Analysis of DNA histograms indicate that the presence of the protector during irradiation reduced the division delay experienced at the G2-M interface. Implications of these effects are discussed.
Sigdestad
C P
CP
Biological & Medical Research Division, Argonne National Laboratory, IL 60439.
Bergquist
B L
BL
Grdina
D J
DJ
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D006224:Cricetinae; D004247:DNA / Q000096:biosynthesis; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D005720:Gamma Rays; D007211:Indoles; D008624:Mercaptoethylamines / Q000494:pharmacology*; D009502:Neutrons; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000494:pharmacology*
D005456:Fluorescent Dyes; D007211:Indoles; D008624:Mercaptoethylamines; D011837:Radiation-Protective Agents; D013438:Sulfhydryl Compounds; C020174:N-(2-mercaptoethyl)-1,3-diaminopropane; C058704:3-(3-methylaminopropylamino)propylmercaptan; C007293:DAPI; D004247:DNA