Rapid detection of antigen-specific mouse IgE by enzyme-linked immunosorbent assay (ELISA).
A rapid, sensitive, antigen-specific mouse IgE capture ELISA is described. A monoclonal rat anti-mouse IgE was used as the capture antibody, and a DNP-coupled BSA-biotinylated conjugate along with a peroxidase-avidin-biotin complex was utilized as the detection system. The lower detection limit of this assay is 8.5 ng/ml of antigen-specific IgE. With some modifications, this assay can be employed to screen for antigen specific antibodies of other isotypes and subtypes.
Strouse
R J
RJ
R.W. Johnson Pharmaceutical Research Institute, Department of Immunopharmacology Raritan, New Jersey 08869.
Anderson
D W
DW
Argentieri
D C
DC
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000941:Antigens; D004140:Dinitrophenols / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D005069:Evaluation Studies as Topic; D005260:Female; D007073:Immunoglobulin E / Q000032:analysis* / Q000592:standards; D051379:Mice
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000941:Antigens; D004140:Dinitrophenols; D007073:Immunoglobulin E