Chloramphenicol resistance mutations in the single 23S rRNA gene of the archaeon Halobacterium halobium.
A broad range of antibiotics affecting protein biosynthesis were screened for their ability to inhibit growth of the archaeon Halobacterium halobium. Only a few drugs, including chloramphenicol, produced inhibitory effects. Mutants which showed increased resistance to chloramphenicol were isolated; of the nine tested, eight exhibited a C----U transition at position 2471 and the ninth had an A----C transversion at position 2088 of 23S rRNA. A double mutant containing both C----U (position 2471) and A----C (position 2088) mutations was isolated, but the level of its chloramphenicol resistance did not exceed that of either single-point mutant. Inferences are made concerning the functional significance of the conserved nucleotides in rRNAs.
Mankin
A S
AS
Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.
Garrett
R A
RA
D001483:Base Sequence; D002470:Cell Survival / Q000187:drug effects; D002701:Chloramphenicol / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial; D006217:Halobacterium / Q000235:genetics*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D012338:RNA, Ribosomal, 23S / Q000235:genetics*
D012338:RNA, Ribosomal, 23S; D002701:Chloramphenicol