Identification of collagens in the human testis.
SDS-PAGE of pepsin-solubilized collagens obtained from testes of elderly men revealed types I, III, V, and VI but not type IV. The total collagen content was 21.3 +/- 1.96% (mean +/- SD) as determined by hydroxyproline assay. The ratio for the pepsin-soluble fraction was 7.98 +/- 3.27%, i.e. very low as compared with other tissues. Type I collagen was determined by SDS-PAGE of cyanogen bromide cleavage of the pepsin-insoluble residue.
Takaba
H
H
Department of Urology, Nagoya University School of Medicine, Japan.
Nagai
T
T
Hashimoto
J
J
Yamamoto
M
M
Miyake
K
K
D000368:Aged; D003094:Collagen / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006909:Hydroxyproline / Q000032:analysis; D008297:Male; D013737:Testis / Q000737:chemistry*
D003094:Collagen; D006909:Hydroxyproline