Establishment of a sarcoma cell line, MS-K, expressing Ki-ras proto-oncogene product from mouse bone marrow stromal cells.
A sarcoma cell line, MS-K, was established from a long-term culture of mouse bone marrow stromal cells. When inoculated into syngeneic 3HC/HeNS1c mice, the cells formed large necrosis-free tumors, but there were no apparent changes in hematological features or in general conditions of tumor-bearing mice. The tumor had a fibroblastic appearance, was well vasculated and differentiated into adipocytes at the peripheral region. Immunohistochemical studies revealed that the cells were positive for vimentin and S-100 protein, indicating that the cells were of lipoblast origin. A significant amount of fat-deposition was induced in the cytoplasm of the cells when MS-K cells were cultured in the presence of hydrocortisone and insulin. Antibody-staining for oncogene products showed that the cells were negative for c-fos but strongly positive for Ki-ras. MS-K cells did not adhere hemopoietic stem cells or support their proliferation, which contrasts with previously established MS-1, which is a hemopoietic-supportive and stem cell-adherent lipoblast cell line. These properties of MS-K and MS-1 should be useful in the identification of the surface structures for stem cell anchorage.
Shirata
K
K
Department of Biology, Faculty of Science, Niigata University, Japan.
Suzuki
T
T
Yanaihara
N
N
Sugimoto
K
K
Mori
K J
KJ
D000818:Animals; D001853:Bone Marrow / Q000737:chemistry / Q000473:pathology*; D002461:Cell Line, Transformed; D005260:Female; D007150:Immunohistochemistry; D051379:Mice; D008809:Mice, Inbred C3H; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics*; D009418:S100 Proteins / Q000737:chemistry; D012513:Sarcoma, Experimental / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D014746:Vimentin / Q000737:chemistry
D009418:S100 Proteins; D014746:Vimentin; D016283:Proto-Oncogene Proteins p21(ras)