Interaction between the left-handed Z-DNA and polyamine. The crystal structure of the d(CG)3 and N-(2-aminoethyl)-1,4-diamino-butane complex.
The DNA fragment d(CG)3 was co-crystallized with N-(2-aminoethyl)-1,4-diaminobutane (PA(24], a chemically synthesized polyamine. The complex crystal contained one polyamine, 3 magnesium cations and one sodium cation per duplex of d(CG)3, and well diffracted the X-ray intensities up to 1.0 A resolution. The d(CG)3 took a left-handed Z-DNA conformation, and the PA(24) molecule electrostatically interacted with the phosphate groups of the d(CG)3 duplex.
Ohishi
H
H
Osaka University of Pharmaceutical Sciences, Japan.
Kunisawa
S
S
van der Marel
G
G
van Boom
J H
JH
Rich
A
A
Wang
A H
AH
Tomita
K
K
Hakoshima
T
T
D003460:Crystallization; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D006860:Hydrogen Bonding; D008274:Magnesium / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry*; D012964:Sodium / Q000737:chemistry; D014961:X-Ray Diffraction
D009838:Oligodeoxyribonucleotides; D010710:Phosphates; D011073:Polyamines; C069659:N-(2-aminoethyl)-1,4-diaminobutane; C041751:tri(deoxycytidylic acid-deoxyguanylic acid); D004247:DNA; D012964:Sodium; D008274:Magnesium