Method for the characterization of stationary phases for the separation of proteins by high-performance liquid chromatography.
A conformational change of lysozyme and myoglobin can be visualized with transition curves in a high-performance liquid chromatographic (HPLC) system using a diode-array detector and UV difference spectrometry. Transition temperatures calculated from the curves are affected by the mobile phase composition and the column packing material. Flow-rates as used in HPLC do not have a measurable influence.
Huisden
R E
RE
Laboratory for Analytical Chemistry, University of Amsterdam, The Netherlands.
Kraak
J C
JC
Poppe
H
H
D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D009113:Muramidase / Q000302:isolation & purification; D009211:Myoglobin / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D009211:Myoglobin; D011506:Proteins; D012997:Solvents; D009113:Muramidase