A skin suction blister model in hairless rats: application to the study of anti-inflammatory and immunomodulatory drugs.
A suction blister model was developed in the hairless rat, in order to study the effects of various agents on the migration of polymorphonuclear leukocytes (PMN). A standardized abrasion, a suction blister, was formed by applying negative pressure to the skin and then separating the epidermis from the dermis. A migration chamber containing serum as the chemoattractant was placed over the wound. After 6 h of migration, the cells in the chamber were harvested, counted and identified. We evaluated PMN migration after treating the animals with active compounds: niflumic acid, and anti-inflammatory drug, and RU 41740, an immunomodulator. This in vivo model provided reproducible data and could be used to study further the functional properties of PMN. In addition, because this assay can also be used in man, a drug found to be effective in the animal system could then be tested for its activity in man.
Rommain
M
M
Laboratoires Cassenne C.R.I. Roussel-Uclaf, Osny, France.
Brossard
C
C
Piron
M A
MA
Smets
P
P
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D001426:Bacterial Proteins / Q000494:pharmacology*; D001768:Blister / Q000209:etiology; D002464:Cell Migration Inhibition; D004341:Drug Evaluation; D005260:Female; D009504:Neutrophils / Q000187:drug effects*; D009544:Niflumic Acid / Q000494:pharmacology*; D051381:Rats; D011923:Rats, Nude; D013396:Suction
D000276:Adjuvants, Immunologic; D001426:Bacterial Proteins; D009544:Niflumic Acid; C037567:glycoprotein, Klebsiella pneumoniae