Comparison of the cytotoxicity of different hydroperoxides to V79 cells.
We compared the cytotoxicity of hydrogen peroxide (H2O2), tertiary butyl hydroperoxide (t-BHP) and methyl linoleate hydroperoxide (MLHP) to V79 cells, using a colony formation assay. In all cases L-buthionine-(S,R)-sulphoximine enhanced the cytotoxicity, and Quin 2 inhibited it. Nordihydroguaiaretic acid (NDGA) and o-phenanthroline suppressed the cytotoxicity of H2O2 and t-BHP, but they had no effect on the cytotoxicity of MLHP. These results suggest that the biological effects of t-BHP are similar to those of H2O2 and not to those of lipid hydroperoxides. In the course of the experiments, we found that NDGA, an antioxidant and food additive, was a potent inhibitor of cytotoxicity of H2O2.
Nakayama
T
T
Department of Food Science and Technology, Faculty of Agriculture, Nagoya University, Japan.
Hori
K
K
Terazawa
K
K
Kawakishi
S
S
D000634:Aminoquinolines / Q000494:pharmacology; D000818:Animals; D019328:Buthionine Sulfoximine; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D003114:Colony-Forming Units Assay; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000633:toxicity; D008054:Lipid Peroxides / Q000633:toxicity; D009637:Masoprocol / Q000494:pharmacology; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives / Q000494:pharmacology; D010545:Peroxides / Q000633:toxicity*; D010618:Phenanthrolines / Q000494:pharmacology; D020122:tert-Butylhydroperoxide
D000634:Aminoquinolines; D005609:Free Radicals; D008054:Lipid Peroxides; D010545:Peroxides; D010618:Phenanthrolines; C008464:methyl linoleate hydroperoxide; D008717:Methionine Sulfoximine; D019328:Buthionine Sulfoximine; D009637:Masoprocol; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; C035739:Quin2; C025205:1,10-phenanthroline