title: Properties of the streptomycete temperate bacteriophage FP43.
abstract: FP43 is a temperate bacteriophage for Streptomyces griseofuscus that forms plaques on many Streptomyces species. FP43 virions contain 56 kb of double-strand DNA that is circularly permuted and terminally redundant, and contains 65% G + C. A physical map of the FP43 genome was constructed, and the origin for headful packaging (pac) was localized to an 8.8-kb region of the genome (hft) that mediates high-frequency transduction by FP43 of plasmid pRHB101. The phage attachment site (attP), a replication origin (rep), a region that inhibits plaque formation (pin), and a 3-kb deletion (rpt) that caused a 100-fold reduction in plasmid transduction were mapped.
authors.affiliation: Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285.
authors.forename: D R
authors.initials: DR
authors.lastname: Hahn
authors.forename: M A
authors.initials: MA
authors.lastname: McHenney
authors.forename: R H
authors.initials: RH
authors.lastname: Baltz
mesh_terms: D001435:Bacteriophages / Q000235:genetics* / Q000502:physiology; D015139:Blotting, Southern; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D010957:Plasmids; D015183:Restriction Mapping; D013302:Streptomyces; D014161:Transduction, Genetic; D010948:Viral Plaque Assay; D014771:Virion / Q000235:genetics
chemical_list: D004279:DNA, Viral