Gene mutation assay of acrolein in the CHO/HGPRT test system.
The mutagenic potential of acrolein has been studied with a wide range of in vitro and in vivo genetic toxicity assays. The data often have been conflicting, especially with the Ames assay. This study was undertaken to assess the mutagenic potential of acrolein using the CHO/HGPRT assay, both with and without metabolic activation. This assay system was chosen because it provides eukaryotic DNA as the target and is capable of detecting a range of mutational events. Because of its considerable toxicity, acrolein was tested over a very narrow dose range of 0.2-2 nl ml-1 without exogenous activation and 0.5-8 nl ml-1 with rat S-9 activation. Multiple assays were performed under both conditions. The results indicated that while acrolein was clearly very cytotoxic, it did not induce a significant mutagenic response in the presence or absence of metabolic activation.
Parent
R A
RA
Consultox, Ltd., Baton Rouge, LA 70898.
Caravello
H E
HE
Harbell
J W
JW
D000171:Acrolein / Q000633:toxicity*; D000818:Animals; D002460:Cell Line; D008040:Genetic Linkage / Q000235:genetics; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D008297:Male; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens; D010641:Phenotype; D051381:Rats; D011919:Rats, Inbred Strains; D014960:X Chromosome / Q000187:drug effects
D009153:Mutagens; D000171:Acrolein; D007041:Hypoxanthine Phosphoribosyltransferase