Effect of recombinant human interleukin-6 on nitrite production of mouse myeloid leukemia cells.
The effect of recombinant human interleukin-6 (rhIL-6) on induction of nitrite (NO(-2)) production was investigated in a mouse myeloid leukemia cell line (M1) and a subclone (Mm1). NO(-2) was induced by rhIL-6 (greater than 50 U/ml) in these cell lines. Pretreatment with rhIL-6 (100 U/ml) for 1 day synergistically enhanced the production of NO(-2) in Mm1 by LPS. Furthermore, pretreatment with IL-6 (100 U/ml) shortened the lag time of induction. These results indicate that IL-6 is involved in the regulation of the NO(-2) production in macrophage-like cells.
Takagi
K
K
Department of Environmental Biochemistry, School of Pharmaceutical Science, University of Shizuoka, Japan.
Hosaka
T
T
Suketa
Y
Y
D000818:Animals; D003470:Culture Media; D006801:Humans; D015850:Interleukin-6 / Q000502:physiology*; D007951:Leukemia, Myeloid; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D009573:Nitrites / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D003470:Culture Media; D015850:Interleukin-6; D008070:Lipopolysaccharides; D009573:Nitrites; D011994:Recombinant Proteins