title: Solid-phase enzyme-immunoassay of anti-insulin antibodies: effect of labeling site in insulin and of labeled number of horseradish peroxidase on the assay sensitivity.
abstract: Three horseradish peroxidase (HRP)-labeled porcine insulins which have definite labeling site(s) were compared regarding sensitivity in a solid-phase enzyme-immunoassay (EIA) of anti-insulin antibodies. The standard curves obtained with LysB29-HRP-insulin and GlyA1-HRP-insulin were steeper than that with GlyA1,LysB29-diHRP-insulin for both polyclonal and monoclonal antibodies. Thus, the mono-HRP-labeled insulins can afford higher sensitivities in the EIA. The importance of the HRP-labeling site in insulin and of the number of labeled HRP was first demonstrated by using HRP-labeled insulins having definite labeling site(s).
authors.affiliation: Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Zaitsu
authors.forename: M
authors.initials: M
authors.lastname: Nakayama
authors.forename: M
authors.initials: M
authors.lastname: Nanami
authors.forename: Y
authors.initials: Y
authors.lastname: Ohkura
mesh_terms: D000818:Animals; D006735:Horseradish Peroxidase; D007124:Immunoenzyme Techniques; D007328:Insulin / Q000032:analysis*; D007330:Insulin Antibodies / Q000032:analysis*; D013552:Swine
chemical_list: D007328:Insulin; D007330:Insulin Antibodies; D006735:Horseradish Peroxidase