Binding of botulinum type Cl, D and E neurotoxins to neuronal cell lines and synaptosomes.
Clostridium botulinum 125I-labeled Cl neurotoxin bound to NG108 hybridoma cell line. Unlabeled type Cl neurotoxin inhibited the binding of the labeled Cl toxin but neither types D nor E toxin. 125I-labeled type D neurotoxin bound to rat brain synaptosomes but did not bind to NG108 cells. It is suggested that receptors for types C and D or E toxins on neuronal cell membranes are different.
Yokosawa
N
N
Department of Microbiology, Sapporo Medical College, Japan.
Tsuzuki
K
K
Syuto
B
B
Fujii
N
N
Kimura
K
K
Oguma
K
K
D000818:Animals; D001667:Binding, Competitive; D001905:Botulinum Toxins / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D051379:Mice; D009447:Neuroblastoma / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D013574:Synaptosomes / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D001905:Botulinum Toxins