title: APGW-amide as an inhibitory neurotransmitter of Achatina fulica Ferussac.
abstract: APGWamide (L-Ala-L-Pro-Gly-L-Trp-NH2) was purified from the ganglia of an African giant snail (Achatina fulica Ferussac). This peptide inhibited (hyperpolarized) more than half of the Achatina neurone types tested. This produced an outward current with the membrane conductance increase of RAPN (right anterior pallial neurone) under voltage clamp. The ED50 of the peptide was 6.2 x 10(-6) M (95% confidence limit: 5.0-7.8 x 10(-6) M) and the Emax was 3.9 +/- 0.2 nA. The effects were due to a membrane permeability increase to K+. The peptide is proposed as an inhibitory neurotransmitter of the Achatina neurones.
authors.affiliation: Department of Physiology, Gifu University School of Medicine, Japan.
authors.forename: G J
authors.initials: GJ
authors.lastname: Liu
authors.forename: D E
authors.initials: DE
authors.lastname: Santos
authors.forename: H
authors.initials: H
authors.lastname: Takeuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Kamatani
authors.forename: H
authors.initials: H
authors.lastname: Minakata
authors.forename: K
authors.initials: K
authors.lastname: Nomoto
authors.forename: I
authors.initials: I
authors.lastname: Kubota
authors.forename: T
authors.initials: T
authors.lastname: Ikeda
authors.forename: Y
authors.initials: Y
authors.lastname: Muneoka
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D004553:Electric Conductivity; D019835:FMRFamide; D005724:Ganglia / Q000502:physiology; D007447:Invertebrate Hormones / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009479:Neuropeptides / Q000302:isolation & purification / Q000494:pharmacology*; D012908:Snails
chemical_list: D007447:Invertebrate Hormones; D009479:Neuropeptides; C063193:alanyl-prolyl-glycyl-tryptophanamide; D019835:FMRFamide