Changes in enzyme activities in skin fibroblasts derived from persons of various ages.
We examined antioxidant enzyme activities (catalase, glutathione peroxidase, and superoxide dismutase) in cultured skin fibroblasts (passage number 2-3) derived from 30 persons of various ages. With increasing ages, catalase activity decreased, glutathione peroxidase activity increased slightly, and superoxide dismutase activity was unchanged. After UVA irradiation (4.8 joule/cm2) of the fibroblasts, only catalase activity decreased by 70%. This suggests that catalase may play an important role in the aging of human skin fibroblasts.
Shindo
Y
Y
Department of Dermatology, Shinshu University School of Medicine, Matsumoto, Japan.
Akiyama
J
J
Yamazaki
Y
Y
Saito
K
K
Takase
Y
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology*; D002374:Catalase / Q000096:biosynthesis; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D002950:Citrate (si)-Synthase / Q000096:biosynthesis; D005347:Fibroblasts / Q000201:enzymology / Q000528:radiation effects; D005649:Fumarate Hydratase / Q000096:biosynthesis; D005956:Glucose-6-Phosphate Isomerase / Q000096:biosynthesis; D005954:Glucosephosphate Dehydrogenase / Q000096:biosynthesis; D006801:Humans; D066298:In Vitro Techniques; D007223:Infant; D007231:Infant, Newborn; D007770:L-Lactate Dehydrogenase / Q000096:biosynthesis; D008875:Middle Aged; D010734:Phosphogluconate Dehydrogenase / Q000096:biosynthesis; D012867:Skin / Q000201:enzymology* / Q000528:radiation effects; D013482:Superoxide Dismutase / Q000096:biosynthesis; D014466:Ultraviolet Rays / Q000009:adverse effects
D007770:L-Lactate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D002374:Catalase; D013482:Superoxide Dismutase; D002950:Citrate (si)-Synthase; D005649:Fumarate Hydratase; D005956:Glucose-6-Phosphate Isomerase