Apolipoprotein B upstream suppressor site: identification of an element which can decrease apolipoprotein B transcription.
Elevated plasma levels of apolipoprotein B (apoB) may predispose to development of premature coronary atherosclerosis. We have identified the first well localized domain of the apoB gene which can effect negative regulation of its transcription. This region binds trans-activating factors present only in apoB producing cell lines. Mutagenesis of this region causes up-regulation of its transcriptional activity. We have termed this element apoB upstream suppressor site (aBUSS) and its trans-activators the apoB repressor proteins (ARP). aBUSS and ARP may play important roles in the transcriptional modulation of apoB.
Ross
R S
RS
Molecular Disease Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland.
Li
A C
AC
Hoeg
J M
JM
Schumacher
U K
UK
Demosky
S J
SJ
Brewer
H B
HB
D000818:Animals; D001055:Apolipoproteins B / Q000235:genetics*; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D002872:Chromosome Deletion; D005786:Gene Expression Regulation; D016153:Genes, Suppressor; D006801:Humans; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013489:Suppression, Genetic; D014158:Transcription, Genetic; D014162:Transfection
D001055:Apolipoproteins B; D015500:Chloramphenicol O-Acetyltransferase