The assay of clozapine and N-desmethylclozapine in human plasma by high-performance liquid chromatography.
A high performance liquid chromatographic method for the simultaneous quantitation of clozapine--a member of the dibenzodiazepine class of antipsychotic drugs--and its reduced metabolite in human plasma has been developed. Clozapine and N-desmethylclozapine are concentrated from blood samples by liquid-liquid extraction, followed by reverse-phase liquid chromatography. Accuracy of the determination is ensured by application of an internal standard, protriptyline, which is closely related to clozapine. A detection limit of 15 ng/mL for clozapine and 30 ng/ml for N-desmethylclozapine was achieved.
Lovdahl
M J
MJ
Department of Psychiatry, College of Medicine, University of Iowa, Iowa City 52242.
Perry
P J
PJ
Miller
D D
DD
D002851:Chromatography, High Pressure Liquid; D003024:Clozapine / Q000031:analogs & derivatives* / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results
C058272:norclozapine; D003024:Clozapine