Involvement of prostaglandin E2 in the tumor promoter phorbol ester-caused increase in epidermal cholesterol sulfotransferase activity.
A single topical application of 10 nmol TPA to mouse skin caused an increase in epidermal cholesterol sulfotransferase activity, a squamous differentiation associated enzyme. A topical application of indomethacin (1.2 mumol), a cyclooxygenase inhibitor, 10 min before TPA application markedly inhibited the above TPA action. Concurrent application of prostaglandin E2 (PGE2) with indomethacin reversed the inhibitory effect of indomethacin. PGF2 alpha, however, failed to reverse the inhibition caused by indomethacin. Both indomethacin and PGE2 alone did not change epidermal cholesterol sulfotransferase activity significantly. These results indicate that PGE2 plays a significant role in the mechanism of TPA-caused increase in cholesterol sulfotransferase activity in mouse epidermis.
Yamamoto
S
S
Department of Pharmacology, School of Medicine, Keio University, Tokyo, Japan.
Jiang
H
H
Kato
R
R
D000818:Animals; D015237:Dinoprost / Q000494:pharmacology; D015232:Dinoprostone / Q000494:pharmacology / Q000502:physiology*; D004817:Epidermis / Q000187:drug effects* / Q000201:enzymology; D005260:Female; D007213:Indomethacin / Q000494:pharmacology; D051379:Mice; D015238:Sulfotransferases / Q000032:analysis*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D015237:Dinoprost; D015238:Sulfotransferases; C043456:cholesterol sulfotransferase; D015232:Dinoprostone; D013755:Tetradecanoylphorbol Acetate; D007213:Indomethacin