Genotoxicity of the boldine aporphine alkaloid in prokaryotic and eukaryotic organisms.
The aporphine alkaloid boldine, present in Peumus boldus (boldo-do-Chile) widely used all over the world, was tested for the presence of genotoxic, mutagenic and recombinogenic activities in microorganisms. This alkaloid did not show genotoxic activity with or without metabolic activation in the SOS chromotest and Ames tester strains TA100, TA98 and TA102. It was not able to induce point and frameshift mutations in haploid Saccharomyces cerevisiae cells. However, mitotic recombinational events such as crossing-over and gene conversion were weakly induced in diploid yeast cells by this alkaloid. Also, boldine was able to induce weakly cytoplasmic 'petite' mutation in haploid yeast cells.
Moreno
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PR
Pós-Graduação em Farmácia-UFRGS, Porto Alegre, RS, Brazil.
Vargas
V M
VM
Andrade
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HH
Henriques
A T
AT
Henriques
J A
JA
D001060:Aporphines / Q000633:toxicity*; D001711:Biotransformation; D003434:Crossing Over, Genetic / Q000187:drug effects; D004272:DNA, Mitochondrial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D005785:Gene Conversion / Q000187:drug effects; D008730:Methoxsalen / Q000633:toxicity; D009152:Mutagenicity Tests; D009153:Mutagens; D013014:SOS Response, Genetics / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects
D001060:Aporphines; D004272:DNA, Mitochondrial; D009153:Mutagens; C011686:boldine; D008730:Methoxsalen