Comparison of the humoral and cytotoxic T-lymphocyte response to individual HLA class I alloantigens in highly immunized patients.
In order to investigate the correlation between activation of cytotoxic T-lymphocyte precursor (CTLp) and the formation of antibodies to alloantigens, we studied 21 highly sensitized patients waiting for a kidney transplantation. Both antibody reactivity and CTLp frequencies of these patients were determined against 88 individual HLA class I alloantigens. A high or low CTLp frequency against a certain HLA-A or -B alloantigen was not correlated with the presence or absence of the antibodies to that antigen. Mismatched antigens, towards which the patient had not formed antibodies, can induce either a higher or a lower frequency of CTLp compared to mismatches towards which the patients had formed antibodies. The possible implications of this lack of correlation between the T- and B-cell allorepertoires with regard to donor selection for (highly immunized) patients is discussed.
Zhang
L
L
Department of Immunohaematology and Blood Bank, University Hospital Leiden, The Netherlands.
van Bree
S
S
Gijbels
Y
Y
van Veen
A
A
van Rood
J J
JJ
Class
F H
FH
D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D006412:Hematopoietic Stem Cells / Q000276:immunology; D006801:Humans; D007114:Immunization; D007518:Isoantibodies / Q000096:biosynthesis; D016030:Kidney Transplantation / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014181:Transplantation Immunology
D015234:HLA-A Antigens; D015235:HLA-B Antigens; D007518:Isoantibodies