title: 11 beta-Hydroxysteroid dehydrogenase mRNA expression in rat kidney.
abstract: 11 beta-Hydroxysteroid dehydrogenase (11 beta-OHSD) protects nonspecific renal mineralocorticoid receptors from exposure to circulating glucocorticoid in vivo by catalyzing the conversion of corticosterone to inactive 11-dehydrocorticosterone. Although 11 beta-OHSD bioactivity and aldosterone binding sites are found in distal tubular cells, mineralocorticoid receptor and 11 beta-OHSD immunoreactivities are not colocalized. However, there are several kidney isoforms of 11 beta-OHSD, not all of which may be immunoreactive, whereas only a single mRNA species has been described. Using in situ hybridization we found 11 beta-OHSD mRNA is highly expressed in all renal tubular epithelia in the rat. It is therefore likely that 11 beta-OHSD is colocalized with mineralocorticoid receptors in distal tubular cells.
authors.affiliation: Department of Medicine, University of Edinburgh, Western General Hospital, United Kingdom.
authors.forename: J L
authors.initials: JL
authors.lastname: Yau
authors.forename: A D
authors.initials: AD
authors.lastname: Van Haarst
authors.forename: M P
authors.initials: MP
authors.lastname: Moisan
authors.forename: S
authors.initials: S
authors.lastname: Fleming
authors.forename: C R
authors.initials: CR
authors.lastname: Edwards
authors.forename: J R
authors.initials: JR
authors.lastname: Seckl
mesh_terms: D042842:11-beta-Hydroxysteroid Dehydrogenases; D000818:Animals; D001345:Autoradiography; D006651:Histocytochemistry; D006913:Hydroxysteroid Dehydrogenases / Q000235:genetics*; D007668:Kidney / Q000378:metabolism*; D008297:Male; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D014018:Tissue Distribution
chemical_list: D012333:RNA, Messenger; D006913:Hydroxysteroid Dehydrogenases; D042842:11-beta-Hydroxysteroid Dehydrogenases