Production of monoclonal antibodies against a hemagglutinin/protease of Vibrio cholerae non-01.
Two hybridoma cell lines producing monoclonal antibodies (MAbs) against a hemagglutinin/protease (HA/P) from Vibrio cholerae non-01 were produced and characterized. The two MAbs contained the kappa light chain and were IgG1 type. They similarly neutralized HA/P protease activity derived from both V. cholerae non-01 and V. cholerae 01, whereas they were unable to neutralize the hemagglutinating activity of HA/P, suggesting that the epitopes for protease and hemagglutination activities are different. Western blotting analysis and the cross-neutralization test with the two MAbs confirmed the identity of HA/P produced by V. cholerae non-01 and 01. This study also suggests that HA/P of V. cholerae and a protease of V. parahaemolyticus are immunologically unrelated.
Honda
T
T
Research Institute for Microbial Diseases, Osaka University, Japan.
Hata-Naka
A
A
Lertpocasombat
K
K
Miwatani
T
T
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D006384:Hemagglutination; D006825:Hybridomas; D008666:Metalloendopeptidases / Q000276:immunology*; D051379:Mice; D009500:Neutralization Tests; D014734:Vibrio cholerae / Q000201:enzymology / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D008666:Metalloendopeptidases; C039814:hemagglutinin-protease