title: Effects of hydrocortisone on the ultrastructure of the thymic cysts of the chick embryo.
abstract: Thymi from 80 19-day chick embryos that were injected with 2.5 mg of hydrocortisone on the 15th day of incubation were examined by light and electron microscopy. Six thymic tissue blocks from each embryo were evaluated. Hydrocortisone caused a marked depression of thymic volume due mainly to the depletion of lymphocytes. Cystic structures, consisting of intercellular and intracellular cysts and individual cystic cells, were more numerous per unit area of medullary tissue in treated embryos than in the controls. In the treated group, the shape of cystic cells varied from squamous to columnar, and hypertrophied columnar cells were also evident. The luminal surface possessed well-developed microvilli with membrane invaginations at their bases. The terminal web was prominent and the lateral plasma membranes were highly interdigitated. The rough and smooth-surface endoplasmic reticula and the Golgi complex of thymi from treated embryos were more developed than those in the controls. Mitochondria and vesicles were increased in number following hydrocortisone treatment. Cysts lined partially by cystic and endothelial-like and smooth muscle cells were encountered in the treated group. These structural modifications probably reflect responses to increased absorptive or transport function. The data suggest that hydrocortisone may alter the structure of cystic cells in chick embryos.
authors.affiliation: Department of Anatomy, National University of Singapore, Kent Ridge.
authors.forename: A S
authors.initials: AS
authors.lastname: Chan
mesh_terms: D000818:Animals; D002642:Chick Embryo / Q000187:drug effects* / Q000648:ultrastructure; D006854:Hydrocortisone / Q000494:pharmacology*; D008854:Microscopy, Electron; D013950:Thymus Gland / Q000187:drug effects / Q000196:embryology* / Q000648:ultrastructure
chemical_list: D006854:Hydrocortisone