Assessment of functional gametes in chickens after transfer of primordial germ cells.
The ability of primordial germ cells (PGCs) transferred from donor to recipient embryos to form functional gametes was assessed using feather colour as a phenotypic marker. Donor primordial germ cells were obtained in blood samples taken from Dwarf White Leghorn embryos, homozygous for the dominant allele at the locus for 'dominant white' plumage (I), which had been incubated for 52 h. Blood samples containing PGCs were transferred by intravascular injection to Barred Plymouth Rock embryos (ii) incubated for 53, 72 and 96 h. Of the embryos which hatched, 28 were male and 31 were female. All chicks were raised to sexual maturity and test mated with Barred Plymouth Rock fowl. All of the 3117 offspring exhibited the typical Barred Plymouth Rock phenotype; no Barred Plymouth Rock x Dwarf White Leghorn chicks were obtained. The results of this study suggest that the frequency of transmission of the donor line genotype after PGC transfer must be improved for this technique to be useful for the routine development of transgenic poultry.
Petitte
J N
JN
Department of Animal and Poultry Science, University of Guelph, Ontario, Canada.
Clark
M E
ME
Etches
R J
RJ
D000818:Animals; D030801:Animals, Genetically Modified; D002642:Chick Embryo; D003433:Crosses, Genetic; D005260:Female; D005718:Gametogenesis / Q000502:physiology*; D005838:Genotype; D005854:Germ Cells / Q000637:transplantation*; D008297:Male; D013997:Time Factors