Mouse T-cell associated serine proteinase 1 degrades collagen type IV: a structural basis for the migration of lymphocytes through vascular basement membranes.
We show that CD8+ T-lymphocyte lines perferentially attach to collagen type IV and that mouse T-cell specific serine proteinase 1 (MTSP-1) preferentially degrades native basement membrane collagen type IV. In contrast, the interstitial collagen types I, II, III, V and VI appear not to be affected. The data reveal that MTSP-1 predominantly cleaves the alpha 2(IV) chain, which is found in the native triple helical structure of type IV collagen in a ratio of alpha 1(IV): alpha 2(IV) = 2:1 into small peptides. The cleavage of the alpha 2(IV) chain within the native collagen type IV molecules most likely results not only in a destabilization of single molecules but of the entire collagenous basement membrane scaffold at the site of MTSP-1 secretion.
Simon
M M
MM
Planck Institut für Immunobiologie, Freiburg, Germany.
Kramer
M D
MD
Prester
M
M
Gay
S
S
D000818:Animals; D001485:Basement Membrane / Q000378:metabolism; D002465:Cell Movement / Q000276:immunology; D003094:Collagen / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004730:Endothelium, Vascular / Q000378:metabolism*; D051379:Mice; D012697:Serine Endopeptidases / Q000502:physiology*; D013601:T-Lymphocytes / Q000201:enzymology* / Q000502:physiology
D003094:Collagen; D012697:Serine Endopeptidases