Minimal biotransformation and toxicity of desflurane in guinea pig liver slices.
Biotransformation and hepatotoxicity of desflurane were evaluated in the guinea pig liver slice culture system. Liver slices (250-300 microns) were prepared from 600-650-g male Hartley guinea pigs. The slices were incubated in sealed vials in a Krebs-Henseleit buffer at 37 degrees C under 95% O2. Desflurane was vaporized to produce media concentrations of 0.7-2.3 mM. After incubation (3-24 h) viability of the slices was determined (K+ content; protein synthesis secretion) along with the biotransformation of desflurane (F-). Isoflurane (2.3 mM) was included in the studies for comparative purposes. Although desflurane caused a mild concentration-related reduction in slice K+ content (1.1-2.2 mM; 20%-40% of control), the effects were less than those produced by 2.3 mM isoflurane (50% of control). High concentrations of desflurane decreased protein synthesis at the first 9 h of incubation, and isoflurane decreased protein synthesis throughout the incubation period. Neither anesthetic affected protein secretion. The biotransformation of desflurane was minimal with threefold less F- produced from desflurane than isoflurane.
Ghantous
H N
HN
Department of Anesthesiology, University of Arizona, College of Medicine, Tucson 85724.
Fernando
J
J
Gandolfi
A J
AJ
Brendel
K
K
D000777:Anesthetics / Q000378:metabolism* / Q000633:toxicity; D000818:Animals; D001711:Biotransformation; D046508:Culture Techniques; D000077335:Desflurane; D006168:Guinea Pigs; D007530:Isoflurane / Q000031:analogs & derivatives* / Q000378:metabolism / Q000633:toxicity; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011188:Potassium / Q000378:metabolism; D014176:Protein Biosynthesis
D000777:Anesthetics; D000077335:Desflurane; D007530:Isoflurane; D011188:Potassium