Interphase cytogenetics reveals somatic pairing of chromosome 17 centromeres in normal human brain tissue, but no trisomy 7 or sex-chromosome loss.
Nuclei isolated from normal human brain tissue, collected from six autopsies, were hybridized with a panel of nine satellite DNA probes specific for the centromeric regions of chromosomes 1, 6, 7, 10, 11, 17, 18, and the X and Y chromosomes. The results did not confirm the recently reported trisomy 7 and loss of sex chromosomes observed in metaphases obtained from normal brain tissue after short-term cultures; however, cells of all six brains displayed somatic pairing of the chromosome 17 centromeres in approximately 50% of the nuclei.
Arnoldus
E P
EP
Department of Neurology, Leiden University, The Netherlands.
Noordermeer
I A
IA
Peters
A C
AC
Raap
A K
AK
Van der Ploeg
M
M
D001921:Brain / Q000648:ultrastructure*; D002886:Chromosomes, Human, Pair 17 / Q000648:ultrastructure*; D002897:Chromosomes, Human, Pair 7; D003582:Cytogenetics / Q000379:methods*; D015342:DNA Probes; D005260:Female; D006801:Humans; D007399:Interphase; D008297:Male; D009693:Nucleic Acid Hybridization; D012730:Sex Chromosomes / Q000648:ultrastructure*; D014314:Trisomy
D015342:DNA Probes