Cell size and cell type analysis of calcitonin gene-related peptide-containing cutaneous and splanchnic sensory neurons in the rat.
Cell size, cell type and calcitonin gene-related peptide (CGRP)-like immunoreactivity were compared between cutaneous and splanchnic sensory neurons by means of a combination of fluorescent tracer and immunohistochemistry. Nineteen percent of cutaneous sensory neurons and 88% of splanchnic sensory neurons were shown to contain CGRP. The former cells were larger than the latter ones, which was also confirmed by the finding that about a half of the former cells contained 200 kDa subunit of neurofilament protein, while only 8% of the latter ones were positively stained. These findings suggest that most of the visceral CGRP-IR sensory neurons are small type B.
Kashiba
H
H
Department of Physiology, Kansai College of Acupuncture Medicine, Osaka, Japan.
Senba
E
E
Ueda
Y
Y
Tohyama
M
M
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis; D008297:Male; D009475:Neurons, Afferent / Q000737:chemistry* / Q000166:cytology; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000294:innervation*; D013153:Splanchnic Nerves / Q000737:chemistry* / Q000166:cytology; D013266:Stilbamidines
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D007381:Intermediate Filament Proteins; D013266:Stilbamidines; D015740:Calcitonin Gene-Related Peptide