Identification of uidA gene sequences in beta-D-glucuronidase-negative Escherichia coli.
A probe specific for the uidA gene of Escherichia coli hybridized with 112 of 116 E. coli isolates examined, including 31 beta-D-glucuronidase-negative and 12 enterohemorrhagic E. coli serotype O157:H7 isolates. Southern hybridizations confirmed the presence of a 900-bp HinfI fragment from the uidA gene in all isolates examined, suggesting that uidA gene sequences are present in most E. coli.
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Division of Microbiology, Food and Drug Administration, Washington, D.C. 20204.
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D001483:Base Sequence; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D005966:Glucuronidase / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization
D015342:DNA Probes; D004269:DNA, Bacterial; D005966:Glucuronidase