title: Interleukin-1 mRNA-expressing macrophages in human chronically inflamed gingival tissues.
abstract: Interleukin-1 (IL-1)-producing cells in inflamed gingival tissues obtained from patients with periodontitis were examined by combined immunohistochemistry and in situ hybridization. Macrophages, T cells, B cells, and endothelial cells were visualized in the lesions by the immunoperoxidase method using specific antibodies to each cell type. Subsequent in situ hybridization using 35S-labeled synthetic oligonucleotide probes complementary to human IL-1 alpha and IL-1 beta mRNA showed IL-1 transcripts in macrophages predominantly but not in T cells or B cells. Neither fibroblasts nor keratinocytes contained the IL-1 message to any significant extent. Endothelial cells also were essentially negative for IL-1 messages. These findings indicate that IL-1 is produced principally by macrophages in inflamed gingival tissues of humans.
authors.affiliation: Department of Periodontology, Niigata University School of Dentistry, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuki
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: K
authors.initials: K
authors.lastname: Hara
mesh_terms: D000328:Adult; D001345:Autoradiography; D002908:Chronic Disease; D005881:Gingiva / Q000378:metabolism* / Q000473:pathology; D005891:Gingivitis / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000235:genetics*; D008264:Macrophages / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*
chemical_list: D007375:Interleukin-1; D012333:RNA, Messenger