Nuclear magnetic resonance spectroscopy of dense cell populations for metabolic studies and bioreactor engineering: a synergistic partnership.
Commercial exploitation of the fruits of recombinant DNA and cell fusion technologies is significantly limited by the lack of fundamental metabolic information on the cell lines of interest, whether these are plant, animal, insect, or microbial cells. NMR can help to provide this information and thereby improve bioreactor design and operation. However, in the case of on-line NMR of dense cell culture devices for metabolic studies, these devices are inherently heterogeneous bioreactors. To ensure that the metabolic information generated is reliable, a number of precautions should be taken. These are the same precautions that should be taken to ensure that commercial bioreactors operate in a reaction-controlled regime. Therefore, reactor engineering methodologies, particularly diffusion and reaction analyses and reaction monitoring by whole-cell NMR must go hand in hand, each extending, complementing, and validating the other.
Dale
B E
BE
Gillies
R J
RJ
D000818:Animals; D001709:Biotechnology; D002477:Cells / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy