Ca2+ is essential for multistep activation of the heat shock factor in permeabilized cells.
We have developed a novel permeabilized-cell system to study transcription mechanisms. In permeabilized cells, heat-induced activation of the heat shock factor and transcription of the hsp70 gene require Ca2+. Activation involves at least two steps: Ca(2+)- and heat-dependent activation of heat shock factor binding and a second step, prior to transcription of hsp70, that requires ATP and is sensitive to genistein, a protein kinase inhibitor.
Price
B D
BD
Joint Center for Radiation Therapy, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115.
Calderwood
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SK
D000818:Animals; D002118:Calcium / Q000494:pharmacology* / Q000502:physiology; D002460:Cell Line; D002463:Cell Membrane Permeability; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000235:genetics*; D006358:Hot Temperature; D007700:Kinetics; D051379:Mice; D008815:Mice, Inbred Strains; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D002118:Calcium