Computer aided tracing and encoding of axonal arborizations.
This paper reports a method of acquiring and codifying branching profiles of axonal arborizations. Using a microcomputer interfaced with a motor driven microscope scanning stage, an acquisition strategy was developed. This strategy allows large profiles (covering up to 1250 mm2) to be measured with an accuracy of a few microns. Each histological section is associated with a specific coordinate system. This system was found to locate the profiles with high precision and reliability in the x-y microscope stage. The overall tracing accuracy of the system is fully discussed.
Ratto
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GM
Istituto di Neurofisiologia CNR, Pisa, Italy.
Usai
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D001369:Axons / Q000648:ultrastructure*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D012160:Retina / Q000166:cytology; D012984:Software