title: 21.1.1, a novel activation marker of T and B cells.
abstract: A new rat mAb designated mAb 21.1.1 was raised against a T cell hybridoma of mouse origin, T2D4. This antibody, an IgG2b, immunoprecipitates from the membrane extracts of iodinated T2D4 cells a 56-kDa glycoprotein of apparent pI 4.6 which gives a 34-kDa polypeptide after treatment with endoglycosidase F. MAb 21.1.1 reacts with an antigen expressed on murine mitogen-activated thymocytes and T cells, and on B cells stimulated by anti-IgM antibodies. Cells isolated from the spleen, lymph nodes and bone marrow are negative, as are purified resting B cells or T cells. This antigen is strongly expressed on most day-16 fetal thymocytes whereas adult thymocytes are almost negative. mAb 21.1.1 may be useful for the study of activation and differentiation of T and B cells.
authors.affiliation: Laboratoire d'Immunologie, Cellulaire et Clinique, INSERM Unité 255, Institut Curie, Paris, France.
authors.forename: V
authors.initials: V
authors.lastname: Malard
authors.forename: S
authors.initials: S
authors.lastname: Amigorena
authors.forename: B
authors.initials: B
authors.lastname: Bauvois
authors.forename: M
authors.initials: M
authors.lastname: Daeron
authors.forename: M A
authors.initials: MA
authors.lastname: Cousin
authors.forename: D
authors.initials: D
authors.lastname: Lando
authors.forename: W H
authors.initials: WH
authors.lastname: Fridman
authors.forename: C
authors.initials: C
authors.lastname: Sautes
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000737:chemistry / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000737:chemistry / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D005434:Flow Cytometry; D007526:Isoelectric Point; D008213:Lymphocyte Activation; D051379:Mice; D008970:Molecular Weight; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000196:embryology / Q000254:growth & development / Q000276:immunology; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte