title: Vaccination of cows with rough Escherichia coli mutants fails to protect against experimental intramammary bacterial challenge.
abstract: Vaccination of cows with rough Escherichia coli mutants fails to protect against experimental intramammary bacterial challenge. Vaccine A, a heat-killed Re mutant of strain K12, (UB 1574), was administered as a single parenteral and local dose to 5 cows with 3 control animals and Vaccine B, a heat-killed mutant of O111:B4 (J5) was administered as three parenteral doses into 5 cows with 5 control animals. Following intramammary challenge with a smooth wild-type strain (P4), an acute, severe clinical mastitis developed in all 14 quarters (9 vaccine A and 5 vaccine B) of the vaccinated animals which was indistinguishable from that in the 11 quarters of the control animals. Following vaccine B there was an elevation in serum IgG1 and IgG2 antibody to the common core antigen of endotoxin which, in contrast to the control animals, showed a further increase after intramammary infection.
authors.affiliation: Institute for Animal Health, Compton, Newbury, Berkshire, UK.
authors.forename: A W
authors.initials: AW
authors.lastname: Hill
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines; D001831:Body Temperature; D002417:Cattle; D002452:Cell Count / Q000662:veterinary; D004926:Escherichia coli / Q000276:immunology* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000517:prevention & control / Q000662:veterinary*; D005260:Female; D008414:Mastitis, Bovine / Q000517:prevention & control*; D008892:Milk / Q000166:cytology / Q000382:microbiology; D014611:Vaccination / Q000662:veterinary*
chemical_list: D001428:Bacterial Vaccines