Metabolic heterogeneity in human calf muscle during maximal exercise.
Human skeletal muscle is composed of various muscle fiber types. We hypothesized that differences in metabolism between fiber types could be detected noninvasively with 31P nuclear magnetic resonance spectroscopy during maximal exercise. This assumes that during maximal exercise all fiber types are recruited and all vary in the amount of acidosis. The calf muscles of seven subjects were studied. Two different coils were applied: an 11-cm-diameter surface coil and a five-segment meander coil. The meander coil was used to localize the 31P signal to either the medial or the lateral gastrocnemius. Maximal exercise, consisting of rapid plantar flexions, resulted in an 83.7% +/- 7.8% decrease of the phosphocreatine pool and an 8-fold increase of the inorganic phosphate (Pi) pool. At rest the Pi pool was observed as a single resonance (pH 7.0). Toward the end of the first minute of exercise, three subjects showed three distinct Pi peaks. During the second minute of exercise the pH values stabilized at 7.12 +/- 0.12, 6.63 +/- 0.15, and 6.27 +/- 0.23. The same pattern was seen when the signal was collected from the medial or lateral gastrocnemius. In four subjects only two distinct Pi peaks were observed. The Pi peaks had differing relative areas in different subjects, but they were reproducible in each individual. This method allowed us to study the appearance and disappearance of the different Pi peaks, together with the changes in pH. Because multiple Pi peaks were seen in single muscles they most likely identify different muscle fiber types.
Vandenborne
K
K
Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia 19104.
McCully
K
K
Kakihira
H
H
Prammer
M
M
Bolinger
L
L
Detre
J A
JA
De Meirlier
K
K
Walter
G
G
Chance
B
B
Leigh
J S
JS
D000227:Adenine Nucleotides / Q000378:metabolism; D004734:Energy Metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007866:Leg; D009682:Magnetic Resonance Spectroscopy; D009132:Muscles / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism; D005082:Physical Exertion
D000227:Adenine Nucleotides; D010710:Phosphates; D010725:Phosphocreatine