Toward a science of metabolic engineering.
Application of recombinant DNA methods to restructure metabolic networks can improve production of metabolite and protein products by altering pathway distributions and rates. Recruitment of heterologous proteins enables extension of existing pathways to obtain new chemical products, alter posttranslational protein processing, and degrade recalcitrant wastes. Although some of the experimental and mathematical tools required for rational metabolic engineering are available, complex cellular responses to genetic perturbations can complicate predictive design.
Bailey
J E
JE
California Institute of Technology, Pasadena 91125.
D000596:Amino Acids / Q000096:biosynthesis; D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods*; D008660:Metabolism; D010100:Oxygen / Q000378:metabolism
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D004798:Enzymes; D010100:Oxygen