Characterization of a murine IgG3 monoclonal antibody against Chlamydia-specific lipopolysaccharide and its purification by affinity chromatography on IgG Fc-receptors from Streptococcus dysgalactiae.
Purification of a murine IgG3 monoclonal antibody (Mab 22) directed against an epitope of Chlamydia-specific lipopolysaccharide by affinity chromatography on recently described solid phase IgG Fc-receptors from Streptococcus dysgalactiae is reported. SDS-PAGE studies revealed the purity of the eluted antibody. The purified Mab 22 was characterized by determination of class, subclass and light chain-type, and by dot tests and immunoblot analysis.
Thiele
D
D
Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität, Giessen, FRG.
Lämmler
C
C
Sting
R
R
Krauss
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002692:Chlamydia trachomatis / Q000276:immunology*; D002691:Chlamydophila psittaci / Q000276:immunology*; D002846:Chromatography, Affinity; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D011961:Receptors, Fc; D013291:Streptococcus
D000911:Antibodies, Monoclonal; D008070:Lipopolysaccharides; D011961:Receptors, Fc