Reversed-phase high-performance liquid chromatography of dinucleoside polyphosphates.
The reversed-phase high-performance liquid chromatographic separation of purine dinucleoside polyphosphates on octadecyl- and phenyl-bonded silica packings using phosphate-based eluents was studied. The effects of pH, ionic strength and the content of the organic modifiers methanol and acetonitrile in the mobile phase on the retention and other chromatographic parameters are reported. The data obtained were used to establish an isocratic assay for diguanosine and diadenosine polyphosphates.
Rotllán Pascual
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Departamento de Bioquímica y Biología Molecular, Universidad de La Laguna, Tenerife, Canary Islands, Spain.
Ramos Carrillo
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Rodríguez del Castillo
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D000313:Adrenal Medulla / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D002837:Chromaffin Granules / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003600:Cytosol / Q000737:chemistry; D015226:Dinucleoside Phosphates / Q000032:analysis* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents
D015226:Dinucleoside Phosphates; D007202:Indicators and Reagents