TaqI digestion reveals fractions of satellite DNAs on human chromosomes.
Restriction endonuclease TaqI has been known as a nonbanding restriction endonuclease when it is used on fixed human chromosomes. However, a specific TaqI digestion can be obtained after varying experimental conditions such as concentration of enzyme, time of incubation, and volume of the final reaction mixture. This digestion consists of an extensive DNA loss in heterochromatin subregions of chromosomes 1, 9, 15, 16, and Y. These regions essentially coincide with those corresponding to the main chromosome locations of satellite II DNA, whose tandem repeated units contain many TaqI target sequences, and some satellite III DNA domains enriched in TaqI sites.
Tagarro
I
I
Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
González-Aguilera
J J
JJ
Fernández-Peralta
A M
AM
D002877:Chromosomes, Human / Q000737:chemistry*; D002878:Chromosomes, Human, Pair 1 / Q000737:chemistry; D002884:Chromosomes, Human, Pair 15 / Q000737:chemistry; D002885:Chromosomes, Human, Pair 16 / Q000737:chemistry; D002899:Chromosomes, Human, Pair 9 / Q000737:chemistry; D004276:DNA, Satellite / Q000032:analysis* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D006570:Heterochromatin; D006801:Humans; D012091:Repetitive Sequences, Nucleic Acid; D014998:Y Chromosome / Q000737:chemistry
D004276:DNA, Satellite; D006570:Heterochromatin; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases