Substantial quantities of the high energy derivative oligophosphoglyceroyl-ATP are located in mitochondria in rat liver.
Perfusion with [8-14C]adenosine demonstrated the likely existence in rat liver of oligophosphoglyceroyl-ATP (OPG-ATP). Purification followed by assay with a new specific 3' phosphodiesterase confirmed this. The quantities present were 5-10-fold those found previously and comparable to total soluble nucleotides. OPG-ATP was also purified from the mitochondrial fraction, shown to co-distribute with succinate dehydrogenase and can be co-purified with an enzyme confined to intermembrane space.
Patel
B
B
Department of Biochemistry, University College London, U.K.
Gove
C D
CD
Hutchinson
W L
WL
Mowbray
J
J
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000032:analysis; D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D005988:Glyceric Acids / Q000032:analysis*; D007425:Intracellular Membranes / Q000737:chemistry; D008297:Male; D008930:Mitochondria, Liver / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000032:analysis*; D010477:Perfusion; D010636:Phenols; D051381:Rats; D011919:Rats, Inbred Strains; D013385:Succinate Dehydrogenase / Q000032:analysis
D005988:Glyceric Acids; D009943:Organophosphorus Compounds; D010636:Phenols; C069271:oligophosphoglycerol-ATP; D000255:Adenosine Triphosphate; D013385:Succinate Dehydrogenase