title: Effect of L-azetidine 2-carboxilic acid on the activity of the general amino-acid permease from Saccharomyces cerevisiae var. ellipsoideus.
abstract: Addition of the L-proline analogue L-azetidine 2-carboxylic acid to growing cultures of Saccharomyces cerevisiae var. ellipsoideus promoted fast deactivation of the general aminoacid permease, measured as L-valine uptake, without an immediate decrease in the growth rate. Cells preincubated with the analogue for 3 h were unable to restore either growth ability or general aminoacid permease activity in analogue-free medium. Eadie-Hofstee plots of L-valine uptake in the presence of the analogue are consistent with a strong reduction in the number of active molecules of the general amino-acid permease located in the plasma membrane. Inhibitory effects on protein synthesis were seen after preincubations of the yeast with the analogue for 3 h although a 30 min preincubation had no effect.
authors.affiliation: Departamento de Bioquímica, Biología Molecular y Fisiología, Facultad de Ciencias, Universidad de Valladolid, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Iglesias
authors.forename: J M
authors.initials: JM
authors.lastname: Ferreras
authors.forename: F J
authors.initials: FJ
authors.lastname: Arias
authors.forename: R
authors.initials: R
authors.lastname: Muñoz
authors.forename: M A
authors.initials: MA
authors.lastname: Rojo
authors.forename: T
authors.initials: T
authors.lastname: Girbés
mesh_terms: D026905:Amino Acid Transport Systems; D000596:Amino Acids / Q000378:metabolism; D001383:Azetidinecarboxylic Acid / Q000494:pharmacology*; D001692:Biological Transport; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D003470:Culture Media; D005656:Fungal Proteins / Q000096:biosynthesis; D026901:Membrane Transport Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development
chemical_list: D026905:Amino Acid Transport Systems; D000596:Amino Acids; D003470:Culture Media; D005656:Fungal Proteins; D026901:Membrane Transport Proteins; D001383:Azetidinecarboxylic Acid