Cross-axis synchronous flow-through coil planet centrifuge (type XLL). II. Speculation on the hydrodynamic mechanism in stationary phase retention.
The hydrodynamic mechanism involved in the retention of stationary phase in the present x-axis coil planet centrifuge system is discussed. A statistical treatment of the retention data disclosed important clues such as the effect of the inward-outward elution mode, the close correlation between the planetary motion and the head-tail elution mode, and the superior retention capacity of the left-handed coils. The combined effects of the radial and lateral centrifugal force field derived from the mathematical analysis of acceleration acting on the coil provide an explanation for the phenomena.
Ito
Y
Y
Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892.
D002498:Centrifugation / Q000295:instrumentation*; D002853:Chromatography, Liquid / Q000295:instrumentation*; D004867:Equipment Design; D008433:Mathematics; D008962:Models, Theoretical