Determination of the antimalarial arteether and its deethylated metabolite dihydroartemisinin in plasma by high-performance liquid chromatography with reductive electrochemical detection.
A rapid, sensitive, and specific reversed-phase HPLC method using reductive electrochemical detection has been developed for the determination of arteether and its O-dealkylated metabolite dihydroartemisinin (DQHS) in plasma. The internal standard assay was validated between 25 and 1000 ng/mL, with a coefficient of variation of greater than 10% for both intra- and interday accuracy and precision. An assay for determination of arteether only, using the n-propyl ether of DQHS as internal standard, was also developed and validated from 5-500 ng/mL. A new automated sample deoxygenation and injection system is used to greatly increase sample throughput. These methods have been used to study the pharmacokinetics of arteether after intravenous and intramuscular administration.
Melendez
V
V
Department of Pharmacology, Walter Reed Army Institute of Research, Washington, DC 20307-5100.
Peggins
J O
JO
Brewer
T G
TG
Theoharides
A D
AD
D000818:Animals; D000962:Antimalarials / Q000097:blood* / Q000493:pharmacokinetics; D037621:Artemisinins; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004285:Dogs; D004563:Electrochemistry / Q000379:methods; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D012717:Sesquiterpenes / Q000097:blood* / Q000493:pharmacokinetics
D000962:Antimalarials; D037621:Artemisinins; D012717:Sesquiterpenes; C039060:artenimol; C055141:artemotil