title: Heterologous transformation of Cochliobolus lunatus.
abstract: A DNA mediated transformation system has been developed for the filamentous fungus Cochliobolus lunatus. Transformants were obtained by using plasmid pAN 7-1 carrying the Escherichia coli hygromycin B phosphotransferase gene (hph) fused to an Aspergillus nidulans promoter. The integration of plasmid pAN 7-1 into the fungal genome altered the ability of this microorganism to transform progesterone.
authors.affiliation: Institute of Biochemistry, Medical Faculty, University of Ljubljana, Yugoslavia.
authors.forename: M
authors.initials: M
authors.lastname: Dermastia
authors.forename: D
authors.initials: D
authors.lastname: Rozman
authors.forename: R
authors.initials: R
authors.lastname: Komel
mesh_terms: D001203:Ascomycota / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D001711:Biotransformation; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics*; D006921:Hygromycin B / Q000494:pharmacology; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011374:Progesterone / Q000378:metabolism; D011523:Protoplasts / Q000187:drug effects / Q000502:physiology; D015183:Restriction Mapping; D014170:Transformation, Genetic
chemical_list: D004271:DNA, Fungal; D006921:Hygromycin B; D011374:Progesterone