Changes of nucleotide patterns in liver, muscle and blood during the growth of Ehrlich ascites cells: application of the reversed-phase and ion-pair reversed-phase high-performance liquid chromatography with radial compression column.
The pool of purine compounds was analysed in liver, skeletal muscle and blood of mice during the growth of Ehrlich ascites tumour cells. Three fast isocratic high-performance liquid chromatographic methods were used. (1) Determination of nucleotides by an isocratic ion-pair reversed-phase chromatography with a 10 mM ammonium phosphate buffer containing acetonitrile and tetrabutylammonium phosphate. (2) Separation of nucleosides and nucleobases in cell extracts by a reversed-phase system with methanol and 50 mM potassium phosphate buffer as eluent. (3) Nucleosides and nucleobases in body fluids were analysed by a reversed-phase system with 10 mM potassium phosphate containing methanol. These methods allow the rapid determination of purine compounds in small biological samples from various cell types and body fluids, with high accuracy and sensitivity. The pool of cellular nucleotides increased during the exponential phase of tumour growth. Adenosine accumulated significantly in all tissues in the stationary phase of tumour growth.
Grune
T
T
Institute of Biochemistry, Medical Faculty (Charité), Humboldt University, Berlin, G.D.R.
Siems
W
W
Gerber
G
G
Tikhonov
Y V
YV
Pimenov
A M
AM
Toguzov
R T
RT
D000818:Animals; D002021:Buffers; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism* / Q000473:pathology; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D007202:Indicators and Reagents; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D009132:Muscles / Q000378:metabolism*; D011687:Purines / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism; D012263:Ribonucleosides / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D012265:Ribonucleotides / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*
D002021:Buffers; D007202:Indicators and Reagents; D011687:Purines; D012263:Ribonucleosides; D012265:Ribonucleotides