Separation of nucleotides in homogenates of octopus retina by ion-pair reversed-phase liquid chromatography and identification by mass spectrometry.
An isocratic ion-pair reversed-phase liquid chromatographic method has been developed for the determination of thirteen nucleotides including cyclic AMP and cyclic GMP. The resolution capability of this method was evaluated successfully using homogenates of octopus retina, the aim being to elucidate the role of nucleotides (particularly ADP and ATP) in the control of oxidative metabolism. To overcome the inherent lack of specificity of ultraviolet detection we used the coupling of liquid chromatography with mass spectrometry, via a thermospray interface, to confirm the identity of the nucleotides of interest in the biological samples.
Fathi
M
M
Central Laboratory for Clinical Chemistry, University Cantonal Hospital, Geneva, Switzerland.
Tsacopoulos
M
M
Raverdino
V
V
Porthault
M
M
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D009711:Nucleotides / Q000032:analysis* / Q000378:metabolism; D049831:Octopodiformes / Q000378:metabolism*; D012160:Retina / Q000737:chemistry* / Q000378:metabolism
D009711:Nucleotides