[Development and clinical evaluation of a new urinary protein test paper (URINE-TP)].
Because standard urinary protein test paper reacts more strongly to albumin than to globulin, we attempted to develop a test paper sensitive to globulin by applying chromatic analysis principles used in quantitative analysis of urinary protein. We developed a new urinary protein test paper (URINE-TP) containing a reagent of ACID VIOLET 17. It was found that URINE-TP reacts with the same strength to gamma-globulin as to albumin. This new test paper therefore allows accurate detection of Bence Jones protein, which was formerly detectable with standard test paper. There was a correlation between values for urinary protein obtained with URINE-TP and those obtained by quantitative analysis. As with standard test paper, URINE-TP indicates urinary protein values in the normal range by a lack of reaction, but on URINE-TP positive and negative results are more clearly distinguishable than on standard test paper. From these results, we conclude that URINE-TP enables detection of both gamma-globulin and albumin in the urine, and that is as sensitive as the analytical method in the screening of urinary proteins.
Kanamori
K
K
Department of Clinical Biochemistry, Faculty of Medicine, Tokyo Medicine and Dental University.
Shiba
K
K
Sugiura
K
K
D001536:Bence Jones Protein / Q000652:urine; D005916:Globulins / Q000652:urine; D006801:Humans; D011507:Proteinuria / Q000175:diagnosis*; D011934:Reagent Strips; D012394:Rosaniline Dyes
D005916:Globulins; D011934:Reagent Strips; D012394:Rosaniline Dyes; C031693:violet BNP; D001536:Bence Jones Protein