"Pocket blotting": a method for transferring nucleic acids onto nylon membranes.
We have developed a fast and efficient method for transferring nucleic acids onto nylon membranes. This method requires less DNA for transfer; no decrease in efficiency is observed after successive probing, and several gels can be processed simultaneously. We believe that this techniques is of general interest in routine analysis of multiple samples in population genetic studies or in diagnosis purposes.
Cuny
G
G
CNRS, UPR 8042, INSERM, U. 249, Intitut de Biologie, Montpellier, France.
Veas
F
F
Roizès
G
G
D002021:Buffers; D004247:DNA / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D005821:Genetic Techniques; D006801:Humans; D008567:Membranes, Artificial; D009693:Nucleic Acid Hybridization; D009757:Nylons; D012685:Sepharose; D013997:Time Factors
D002021:Buffers; D008567:Membranes, Artificial; D009757:Nylons; D004247:DNA; D012685:Sepharose