Effects of Ca2+ channel ligands on [3H]QNB binding at m1 and m3 muscarinic receptors.
1. The effects of Ca2+ channel ligands on [3H]QNB binding in m1- or m3-transfected Chinese hamster ovary (CHO) cells have been studied. 2. The IC50 values of Ca2+ channel ligands for the inhibition of [3H]QNB binding were between 10(-6) and 10(-4) M and the rank order of potency was HOE 166 greater than McN 6186 greater than nicardipine greater than tiamdipine greater than verapamil greater than diltiazem greater than Bay K 8644 greater than nifedipine at m1 and m3 receptors. 3. The results indicate that Ca2+ channel ligands employed in this experiment do not distinguish subtypes of muscarinic receptors.
Kwon
Y W
YW
Department of Biochemical Pharmacology, School of Pharmacy, State University of New York, Buffalo 14260.
Triggle
D J
DJ
D000818:Animals; D002120:Calcium Channel Agonists / Q000494:pharmacology*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002478:Cells, Cultured; D006224:Cricetinae; D005260:Female; D010053:Ovary / Q000187:drug effects / Q000378:metabolism; D011813:Quinuclidinyl Benzilate / Q000378:metabolism*; D051381:Rats; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000378:metabolism*
D002120:Calcium Channel Agonists; D002121:Calcium Channel Blockers; D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate