Rapid and simple high-performance liquid chromatographic determination of tricyclic antidepressants for routine and emergency serum analysis.
An isocratic reversed-phase high-performance liquid chromatographic procedure is presented for the simultaneous detection of desipramine, nortriptyline, imipramine, amitriptyline and clomipramine in serum. Drugs are extracted after sample alkalinization and separated from each other on an octyl reversed-phase with n-butylamine as mobile phase modifier. Detection is achieved at 254 nm. The recovery of tricyclic antidepressants (92-110%) has good precision, with a relative standard deviation of less than 5%. Being rapid and simple, the method is suitable for the emergency clinical laboratory.
Segatti
M P
MP
Laboratorio di Analisi Chimico Cliniche, Ospedale Infantile Burlo Garofolo, Trieste, Italy.
Nisi
G
G
Grossi
F
F
Mangiarotti
M
M
Lucarelli
C
C
D000929:Antidepressive Agents, Tricyclic / Q000097:blood*; D002648:Child; D002851:Chromatography, High Pressure Liquid; D004630:Emergencies; D006801:Humans; D013056:Spectrophotometry, Ultraviolet
D000929:Antidepressive Agents, Tricyclic