Endothelin-1 inhibits adipogenic differentiation of 3T3-L1 preadipocytes.
The effect of endothelin (ET)-1 on the adipogenic differentiation of 3T3-L1 preadipocytes was examined. Cellular morphology and lipoprotein lipase activity were used as differentiation markers. ET-1 inhibited the hormone-induced adipogenic differentiation of 3T3-L1 preadipocytes morphologically and biochemically in a dose-dependent manner. These findings promote ET-1 as a potent inhibitor of adipogenic differentiation, playing an important role in cellular differentiation of preadipocytes and making it a significant regulator of lipid metabolism.
Tanahashi
T
T
Growth Factor Division, National Cancer Center Research Institute, Tokyo, Japan.
Yamaguchi
K
K
Ishikawa
S
S
Kusuhara
M
M
Adachi
I
I
Abe
O
O
D000273:Adipose Tissue / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology; D000818:Animals; D015415:Biomarkers; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D016232:Endothelins / Q000494:pharmacology*; D008071:Lipoprotein Lipase / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains
D015415:Biomarkers; D016232:Endothelins; D008071:Lipoprotein Lipase; D002118:Calcium