Immunohistochemical detection of DNA alkylation adducts in rat and hamster liver after treatment with dimethylnitrosamine.
Monoclonal and polyclonal antibodies specific for methylation adducts have been applied in an immunohistochemical study of DNA damage in rat and hamster liver following exposure to dimethylnitrosamine. The approach was validated, for frozen and paraffin-embedded sections, by comparison with biochemical data on adduct levels in whole tissues or specific cell populations in the same experimental systems. The potential application of this method to human exposure assessment is discussed.
Asamoto
M
M
Unit of Mechanisms of Carcinogenesis, International Agency for Research on Cancer, Lyon, France.
Mikheev
A M
AM
Jiang
Y Z
YZ
Wild
C P
CP
Hall
J
J
Montesano
R
R
D000478:Alkylation; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D006224:Cricetinae; D004249:DNA Damage; D004128:Dimethylnitrosamine / Q000494:pharmacology*; D007150:Immunohistochemistry; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D008780:Methyltransferases / Q000276:immunology / Q000378:metabolism*; D019853:O(6)-Methylguanine-DNA Methyltransferase; D051381:Rats
D000911:Antibodies, Monoclonal; D008780:Methyltransferases; D019853:O(6)-Methylguanine-DNA Methyltransferase; D004128:Dimethylnitrosamine