Association of exogenous DNA with cattle and insect spermatozoa in vitro.
Spermatozoa isolated from domestic cattle (Bos taurus), the Australian sheep blowfly (Lucilia cuprina), and the honeybee (Apis mellifera) are capable of binding exogenous radiolabeled linear DNA. Both motile and nonmotile bovine sperm exhibit four distinct patterns of DNA association. Following treatment with DNase I, the relative proportion of one of these patterns increases specifically in living sperm, suggesting that a small proportion of DNA that associates with bovine sperm may be sequestered within the sperm head.
Atkinson
P W
PW
CSIRO Division of Entomology, Canberra, NSW, Australia.
Hines
E R
ER
Beaton
S
S
Matthaei
K I
KI
Reed
K C
KC
Bradley
M P
MP
D000818:Animals; D001345:Autoradiography; D001516:Bees; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism; D004175:Diptera; D008297:Male; D013077:Sperm Head / Q000378:metabolism; D013094:Spermatozoa / Q000378:metabolism*; D014170:Transformation, Genetic
D004247:DNA; D003850:Deoxyribonuclease I