A "slow" temperature jump apparatus built from a stopped-flow machine.
A simple modification to a standard thermostated stopped-flow machine is described which allows it to be used as a temperature jump machine. Temperature jumps larger than 10 degrees C can be achieved in less than 150 ms which makes it useful for the range of times where conventional rapid temperature jumps are not applicable. The apparatus has a sample size of 300 microliters and can produce temperature jumps both above and below the initial temperature.
Goldmann
W H
WH
University of Bristol, Department of Biochemistry, United Kingdom.
Geeves
M A
MA
D000199:Actins / Q000378:metabolism; D000818:Animals; D015879:Myosin Subfragments / Q000378:metabolism; D011817:Rabbits; D013053:Spectrophotometry / Q000295:instrumentation*; D013696:Temperature
D000199:Actins; D015879:Myosin Subfragments