title: Factors contributing to chromosome damage in lymphocytes of cigarette smokers.
abstract: Cigarette smoking is generally believed to be responsible for a substantial number of human health problems. However, the causal relationship between smoking, the induction of biological effects and the extent of health problems among smokers have not been fully documented. Using the recently developed lymphocyte micronucleus (MN) assay, we have evaluated the chromosome aberration frequencies in 67 cigarette smokers and 59 matched non-smoking control subjects. We found that the mean MN frequency (per 100 cells) in the smokers was slightly higher than that found in the non-smokers (0.71 +/- 0.23 and 0.58 +/- 0.05 respectively; p less than 0.08). Factors which contribute to the expression of chromosome aberrations were also investigated. A significant age-dependent increase in MN frequencies was observed in both groups (p less than 0.05). Linear regression analysis showed that the age-dependent effects among smokers (r = 0.54; p less than 0.02) was further enhanced by cigarette consumption (r = 0.62; p less than 0.005). Consumption of low potency 'one-a-day' type multivitamins had no effect on MN frequencies in either sex of non-smokers and in the 1 male smoker who took multivitamins but vitamin intake consistently reduced the MN frequencies among female smokers. Using a challenge assay, fidelity of DNA repair was evaluated. Lymphocytes from both smokers and non-smokers were irradiated with single doses of 0 or 100 cGy of X-rays or with double doses of 100 cGy of X-rays each separated by 15 or 60 min (100/15 or 100/60). Chromosome translocation frequencies were consistently higher after irradiation in lymphocytes from smokers than in those from non-smokers. Statistically significant differences were detected when the cells were irradiated with the double doses of 100 cGy X-rays each separated by 60 min (p less than 0.05). These data suggest that lymphocytes from smokers made more mistakes in the repair of DNA damage than cells from non-smokers. Our studies provide new insights into the genotoxic effects of cigarette smoke and new information which may be useful for understanding the mechanisms for induction of health problems from smoking.
authors.affiliation: Department of Preventive Medicine and Community Health, University of Texas Medical Branch, Galveston 77550.
authors.forename: W W
authors.initials: WW
authors.lastname: Au
authors.forename: D M
authors.initials: DM
authors.lastname: Walker
authors.forename: J B
authors.initials: JB
authors.lastname: Ward
authors.forename: E
authors.initials: E
authors.lastname: Whorton
authors.forename: M S
authors.initials: MS
authors.lastname: Legator
authors.forename: V
authors.initials: V
authors.lastname: Singh
mesh_terms: D000375:Aging; D002869:Chromosome Aberrations / Q000276:immunology*; D004260:DNA Repair; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects; D008297:Male; D015162:Micronucleus Tests; D012907:Smoking / Q000009:adverse effects*; D014178:Translocation, Genetic; D014815:Vitamins / Q000008:administration & dosage
chemical_list: D014815:Vitamins