Monitoring of effects of cis-diamminedichloroplatinum (II). Part V. Comparison of in vitro Pt-cytostatic effect on blastic transformation of human and murine cells.
The effect of oxoplatinum on blastic transformation of human peripheral blood mononuclear cells (PBMC) was monitored following phytohemagglutinin (PHA) stimulation. The results were compared with those published in part III of the present work as well as with the effect of cisplatinum and carboplatinum on the blastic transformation of human cells. In addition, the influence of three Pt-cytostatics on blastic transformation (BT) of PHA pretreated murine splenic cells was studied, and a comparison of sensitivity to cytostatics was made between the murine splenic and human peripheral cells. Cisplatinum demonstrated the highest toxicity, and carboplatinum showed minimum toxicity. The highest inhibitory effect was noted when cytostatics were applied either simultaneously with, or 48 h after PHA, i.e., at the stage of maximum proliferating activity. The murine spleen cells showed higher susceptibility to the effect of Pt-cytostatics in blastic transformation test than human peripheral cells. The murine spleen nuclear cells revealed deeper BT inhibition at the same concentration of substance than the human PBMC.
Kanková-Vancurová
M
M
Faculty of Pharmacy, Charles University, Hradec Králove, Czechoslovakia.
Procházková
J
J
D000818:Animals; D001752:Blast Crisis / Q000188:drug therapy*; D016190:Carboplatin / Q000627:therapeutic use; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000031:analogs & derivatives / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D051379:Mice; D008809:Mice, Inbred C3H; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology; D010835:Phytohemagglutinins / Q000494:pharmacology; D013154:Spleen / Q000187:drug effects / Q000276:immunology
D010835:Phytohemagglutinins; D016190:Carboplatin; D002945:Cisplatin; C043804:diamminedichlorodihydroxyplatinum IV