Balance between fatty acid degradation and lipid accumulation in cultured smooth muscle cells and IC-21 macrophages exposed to oleic acid.
1. The effect of changes in fatty acid beta-oxidation activity on triglyceride and cholesteryl ester synthesis were studied in cultured smooth muscle cells (SMC) and in a macrophage cell line IC-21 in the presence of oleic acid (100 microM). 2. Etomoxir, an inhibitor of carnitine palmitoyltransferase I, stimulated the incorporation of [2-3H]glycerol into triglycerides in SMC and in macrophages 6.2- and 8.2-fold, respectively, and the incorporation of [4-14C]cholesterol into cholesteryl esters in macrophages 3.5-fold. 3. L-Carnitine, a cofactor of fatty acid beta-oxidation, decreased the incorporation of [2-3H]glycerol into triglycerides in smooth muscle cells by 69% and the incorporation of [4-14C]cholesterol into cholesteryl esters by 52%. L-Carnitine had no effect on the macrophages.
Moinat
M
M
Département de Biochimie Médicale, Centre Médical Universitaire, Geneva, Switzerland.
Kossovsky
M
M
Chevey
J M
JM
Giacobino
J P
JP
D000818:Animals; D002331:Carnitine / Q000494:pharmacology; D002334:Carnitine O-Palmitoyltransferase / Q000037:antagonists & inhibitors; D002460:Cell Line; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism; D002788:Cholesterol Esters / Q000096:biosynthesis; D003331:Coronary Vessels; D004852:Epoxy Compounds / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D050356:Lipid Metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D013552:Swine; D014280:Triglycerides / Q000096:biosynthesis
D002788:Cholesterol Esters; D004852:Epoxy Compounds; D005227:Fatty Acids; D014280:Triglycerides; D002784:Cholesterol; D002334:Carnitine O-Palmitoyltransferase; C054207:etomoxir; D005990:Glycerol; D002331:Carnitine