Genomic rearrangement of the c-myc proto-oncogene in non-AIDS-related lymphoma in Japan.
Southern blot analysis was employed to analyze the structural alterations of the c-myc oncogene in genomic DNA derived from tumor specimens of 35 adults with pathologically classified and immunophenotyped non-AIDS-related, non-Hodgkin's lymphoma in Japan. In this study, seven cases (20%), including one peripheral T-cell lymphoma and six B-cell lymphomas of various histological types, were demonstrated to have additional c-myc fragments. An interesting feature is that c-myc rearrangements were found in three out of eight primary gastrointestinal lymphomas. Analyses with several restriction enzymes revealed that the breakpoints in these cases were clustered in a region spanning the first exon, first intron and nearby 5'-flanking sequences of the c-myc gene, suggesting that the alteration of this region may represent an important molecular event in activating the oncogenic potential of the c-myc gene.
Nagai
M
M
First Department of Internal Medicine, Kagawa Medical School, Japan.
Ikeda
K
K
Tasaka
T
T
Irino
S
S
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015139:Blotting, Southern; D002873:Chromosome Fragility; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D015321:Gene Rearrangement; D016259:Genes, myc; D006801:Humans; D007564:Japan; D016393:Lymphoma, B-Cell / Q000235:genetics; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics* / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000235:genetics; D008297:Male; D008875:Middle Aged; D005810:Multigene Family; D000090063:Proto-Oncogene Mas; D015183:Restriction Mapping
D004273:DNA, Neoplasm; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas