A sensitive immunochemiluminescence assay for human serum amyloid A protein.
We describe an immunochemiluminescence assay for human plasma serum amyloid A protein (SAA) in which specific rabbit polyclonal antibodies against synthetic peptides are used. The detection of the antigen-antibody reaction at 425 nm is based on a brief emission of light by a luminophor component (signal) in response to chemical energy. The working range of the assay covers plasma SAA concentrations from 5 to 100 micrograms/L. The lower detection limit is 5 micrograms/L, the within- and between-assay CVs are less than 12%. Bilirubin, cholesterol and triglyceride in final concentrations of up to 220 mumol/L, 8.1 mmol/L and 2.68 mmol/L, respectively, do not interfere with the assay. Results were correlated with those obtained by the enzyme-linked immunosorbent assay using the same antibodies (r = 0.95; p less than 0.001; n = 50). This method is inexpensive, simple and easily automated.
Hachem
H
H
Departement de Biologie Clinique, Centre Claudius Regaud, Toulouse, France.
Saile
R
R
Favre
G
G
Bosco
R
R
Raynal
G
G
Fruchart
J C
JC
Soula
G
G
D000906:Antibodies; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements; D008165:Luminol; D000685:Serum Amyloid A Protein / Q000032:analysis* / Q000276:immunology
D000906:Antibodies; D000685:Serum Amyloid A Protein; D008165:Luminol