Differential penetration of targeting agents into multicellular spheroids derived from human neuroblastoma.
We have used a multicellular tumour spheroid model for determination of the penetration of various targeting agents of potential use in the treatment of neuroblastoma. Both the radiopharmaceutical meta-iodobenzylguanidine (mIBG) and the beta subunit of nerve growth factor (beta-NGF) distributed uniformly throughout spheroids, though the latter was poorly concentrated relative to mIBG. In contrast, the anti-neuroectodermal monoclonal antibody. UJ13A bound only to peripheral cell layers with little accumulation in the spheroid interior. Differential penetration of targeting agents may influence the choice of conjugated radionuclide which is likely to achieve maximum therapeutic benefit.
Mairs
R J
RJ
Department of Radiation Oncology, University of Glasgow.
Angerson
W J
WJ
Babich
J W
JW
Murray
T
T
D019797:3-Iodobenzylguanidine; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000627:therapeutic use; D001692:Biological Transport; D002460:Cell Line; D002648:Child; D004337:Drug Carriers; D006801:Humans; D007124:Immunoenzyme Techniques; D007457:Iodine Radioisotopes / Q000008:administration & dosage* / Q000627:therapeutic use; D007462:Iodobenzenes / Q000008:administration & dosage* / Q000378:metabolism / Q000627:therapeutic use; D008954:Models, Biological; D009414:Nerve Growth Factors / Q000008:administration & dosage* / Q000378:metabolism / Q000627:therapeutic use; D009447:Neuroblastoma / Q000000981:diagnostic imaging / Q000473:pathology / Q000532:radiotherapy; D011877:Radionuclide Imaging
D000911:Antibodies, Monoclonal; D004337:Drug Carriers; D007457:Iodine Radioisotopes; D007462:Iodobenzenes; D009414:Nerve Growth Factors; D019797:3-Iodobenzylguanidine