Glycosyltransferase activities in chondrocytes from osteoarthritic and normal human articular cartilage.
Six glycosyltransferases (mannosyl-, glucosyl-, N-acetyl-glucosaminyl-, galactosyl-, sialyl- and fucosyltransferases) are studied and characterized for their optimal conditions and their relations with interfering reactions (glycosyl-nucleotide pyrophosphatases, glycosidases and proteinases) in chondrocytes from osteoarthritic and normal human articular cartilage. Osteoarthritis induces increased activities for five glycosyl-transferases. The observed modifications are not explained by alterations in physico-chemical parameters of the enzymes or by intervention of glycosyl-nucleotide pyrophosphatases, glycosidases or proteolytic enzymes.
Richard
M
M
Department of General and Medical Biochemistry, INSERM-CNRS U189. Lyon-Sud Medical School, Oullins, France.
Vignon
E
E
Peschard
M J
MJ
Broquet
P
P
Carret
J P
JP
Louisot
P
P
D002358:Cartilage, Articular / Q000166:cytology / Q000201:enzymology*; D006031:Glycosylation; D006602:Hexosyltransferases / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D010003:Osteoarthritis / Q000201:enzymology*; D012016:Reference Values
D006602:Hexosyltransferases; C018660:asparagine-N-glycosyltransferase