[Prenatal sex identification with biotinylated human Y-specific DNA probe].
Using biotinylated human Y-specific DNA probe PHY2.1 and Cos 84, we carried out Southern and dot hybridization with human DNA from artificially aborted chorionic villi, and with DNA of peripheral lymphocytes from normal male and female. The results show that PHY2.1 may be applied in prenatal sex identification both by Southern and by dot hybridization but with Cos 84, only Southern hybridization could be used.
Dong
W
W
Department of Medical Genetics, Chengdu First Municipal Hospital.
Jiang
S
S
Zhang
S
S
Zhao
L
L
D001710:Biotin; D015139:Blotting, Southern; D015342:DNA Probes; D005260:Female; D006801:Humans; D008297:Male; D009943:Organophosphorus Compounds / Q000138:chemical synthesis; D011296:Prenatal Diagnosis; D012732:Sex Determination Analysis; D014050:Toluene / Q000031:analogs & derivatives / Q000138:chemical synthesis; D014998:Y Chromosome
D015342:DNA Probes; D009943:Organophosphorus Compounds; D014050:Toluene; D001710:Biotin; C065428:4-toluenesulfinic acid