title: Bacterially expressed nucleoprotein of infectious hematopoietic necrosis virus augments protective immunity induced by the glycoprotein vaccine in fish.
abstract: The ribonucleoprotein gene of infectious hematopoietic necrosis virus (IHNV) has been expressed in Escherichia coli as a trpE fusion protein. This viral protein does not induce protective immunity to lethal IHNV infection in fish, and virus-neutralizing antibodies do not react with this viral protein. However, when it was administered with a bacterial lysate containing a region of the IHNV glycoprotein, there was enhanced resistance in immunized fish to lethal virus infection.
authors.affiliation: Department of Microbiology, Oregon State University, Corvallis 97331-3804.
authors.forename: L A
authors.initials: LA
authors.lastname: Oberg
authors.forename: J
authors.initials: J
authors.lastname: Wirkkula
authors.forename: D
authors.initials: D
authors.lastname: Mourich
authors.forename: J C
authors.initials: JC
authors.lastname: Leong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D005393:Fish Diseases / Q000517:prevention & control*; D015870:Gene Expression; D006023:Glycoproteins / Q000276:immunology; D007114:Immunization / Q000662:veterinary; D008969:Molecular Sequence Data; D010957:Plasmids; D012367:RNA, Viral / Q000737:chemistry; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012209:Rhabdoviridae / Q000235:genetics / Q000276:immunology*; D012261:Ribonucleoproteins / Q000235:genetics / Q000276:immunology*; D014337:Trout; D014614:Vaccines, Synthetic; D014765:Viral Vaccines; D014777:Virus Diseases / Q000517:prevention & control / Q000662:veterinary*
chemical_list: D004279:DNA, Viral; D006023:Glycoproteins; D012367:RNA, Viral; D011994:Recombinant Proteins; D012261:Ribonucleoproteins; D014614:Vaccines, Synthetic; D014765:Viral Vaccines