Production of intracellular arachidonic acid metabolites by human granulosa cells.
The major intracellular metabolites of arachidonic acid within human granulosa cells are an epoxy-eicosatrienoic acid (EET), and a dihydroxy-metabolite. The former which was present at higher levels, co-migrated with 5,6-EET on HPLC. Incubation of the cells with LH for 5 min stimulated the production of both 5,6-EET and the dihydroxy compound 2-4 fold. The production of other intracellular arachidonic acid metabolites was unaffected by stimulation with LH. These results suggest that one or both of these metabolites may have a role in steroidogenesis in human granulosa cells.
Zosmer
A
A
Institute of Obstetrics and Gynaecology, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Elder
M G
MG
Sullivan
M H
MH
D001095:Arachidonic Acids / Q000096:biosynthesis*; D005260:Female; D005307:Fertilization in Vitro; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007986:Luteinizing Hormone / Q000494:pharmacology
D001095:Arachidonic Acids; D007986:Luteinizing Hormone