Phenytoin-induced DNA synthesis and inositol 1,4,5-trisphosphate formation in L-929 fibroblasts.
Culture of L-929 fibroblasts in the presence of phenytoin (2.5-5.0 micrograms/ml) increased DNA synthesis, as indicated by increased [3H]thymidine uptake, while a higher dose (20 micrograms/ml) inhibited DNA synthesis. In like manner, a low dose of phenytoin (5.0 micrograms/ml) was effective in increasing inositol 1,4,5-trisphosphate formation while a higher dose (10 micrograms/ml) tended to inhibit this activity. These data suggest that the formation of inositol phosphate second messengers may play a role in phenytoin-induced fibroblast proliferation and connective tissue growth.
Dill
R E
RE
Department of Anatomy, Baylor College of Dentistry, Dallas, Texas 75246.
Farmer
G R
GR
D001839:Bombesin / Q000494:pharmacology; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D005347:Fibroblasts / Q000378:metabolism*; D015544:Inositol 1,4,5-Trisphosphate / Q000096:biosynthesis*; D010672:Phenytoin / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism
D010672:Phenytoin; D015544:Inositol 1,4,5-Trisphosphate; D004247:DNA; D001839:Bombesin; D013936:Thymidine