title: Intraerythrocytic administration of a synthetic Plasmodium antigen elicits antibody response in mice, without carrier molecules or adjuvants.
abstract: The synthetic peptide (NANP)40, reproducing the tandem-repeated epitope of the circumsporozoite protein of Plasmodium (Laverania) falciparum, was entrapped into murine, autologous erythrocytes by a hypotonic dialysis method. Mice immunized intravenously with minute amounts of encapsulated peptide produced considerable antibody titres. This result indicates that intraerythrocytic antigen administration may have a potential as an immunization system for humans, since it dispenses with adjuvants and carrier molecules.
authors.affiliation: Dipartimento di Biologia Molecolare, Universita' di Camerino, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Esposito
authors.forename: A M
authors.initials: AM
authors.lastname: Santroni
authors.forename: A
authors.initials: A
authors.lastname: Habluetzel
authors.forename: V
authors.initials: V
authors.lastname: Grelloni
authors.forename: A
authors.initials: A
authors.lastname: Pessi
authors.forename: S
authors.initials: S
authors.lastname: Lombardi
authors.forename: G
authors.initials: G
authors.lastname: Falcioni
authors.forename: M
authors.initials: M
authors.lastname: Brunori
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D000953:Antigens, Protozoan / Q000138:chemical synthesis / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology*; D007114:Immunization / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D010963:Plasmodium falciparum / Q000276:immunology*; D015800:Protozoan Proteins
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015800:Protozoan Proteins; C041595:circumsporozoite protein, Protozoan