Determination of aluminum in bone in haemodialyzed patients, using inductively coupled argon plasma emission spectrometry.
We propose a new method for measuring aluminium in bone tissue, using argon plasma emission spectrophotometry. The detection limit in the bone nitric digestion liquid was 0.015 mumol/l (corresponding to 0.0075 mumol/g, i.e. 0.2 microgram/g for a tissue sample with 1.0 g wet weight). Within-run CVs were 4.66% and 1.43% for tissues containing 0.15 and 0.64 mumol/g, respectively. Other bone constituents such as calcium, phosphorus, magnesium, sodium, potassium, do not affect aluminum results. Normal values obtained in bone of subjects not suspected of aluminium intoxication were: mean +/- SD; 0.09 +/- 0.044 mumol/g (n = 24). In dialysis patients we found a mean bone aluminium content of 0.75 mumol/g for concentrations ranging between 0.16 and 3.38 mumol/g.
Leflon
P
P
Biochemistry Laboratory, University Hospital, Amiens, France.
Plaquet
R
R
Morinière
P
P
Fournier
A
A
D000535:Aluminum / Q000032:analysis*; D001128:Argon; D001842:Bone and Bones / Q000737:chemistry*; D002118:Calcium / Q000032:analysis; D006801:Humans; D008903:Minerals / Q000032:analysis; D012016:Reference Values; D006435:Renal Dialysis; D013054:Spectrophotometry, Atomic; D013057:Spectrum Analysis / Q000706:statistics & numerical data
D008903:Minerals; D001128:Argon; D000535:Aluminum; D002118:Calcium