title: Determination of onapristone and its N-desmethyl metabolite in human plasma or serum by high-performance liquid chromatography.
abstract: An automated reversed-phase high-performance liquid chromatographic method for the determination of the antiprogestin onapristone and its N-desmethyl metabolite in human plasma or serum is described. Ultraviolet detection was performed at 315 nm, with a limit of detection of 1 ng/ml at a signal-to-noise ratio of 3. The intra- and inter-assay precision were less than or equal to 6% and less than or equal to 7%, respectively. Onapristone and its N-desmethyl metabolite were stable in human plasma. The method was successfully applied to serum samples obtained from human volunteers after oral administration of onapristone.
authors.affiliation: Schering AG, Pharmacokinetics, Berlin, F.R.G.
authors.forename: C
authors.initials: C
authors.lastname: Zurth
authors.forename: F
authors.initials: F
authors.lastname: Kagels
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D004355:Drug Stability; D005615:Freezing; D006067:Gonanes / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D013696:Temperature
chemical_list: D006067:Gonanes; D007202:Indicators and Reagents; C068050:ZK 115716; C053238:onapristone