Partition of macromolecules and cell particles in aqueous two-phase systems based on hydroxypropyl starch and poly(ethylene glycol).
The partition behavior of proteins, nucleic acids, cell membranes, cell organelles and whole cells has been studied in liquid-liquid two-phase systems composed of water, poly-(ethylene glycol), and an hydroxypropyl starch. The properties of the systems are in many respects comparable with the traditional poly(ethylene glycol)-dextran systems, but the cost is reduced to around one-fifth.
Sturesson
S
S
Hyclone AB, Lund, Sweden.
Tjerneld
F
F
Johansson
G
G
D002458:Cell Fractionation / Q000379:methods*; D002462:Cell Membrane / Q000648:ultrastructure; D002736:Chloroplasts / Q000648:ultrastructure; D007202:Indicators and Reagents; D009696:Nucleic Acids / Q000302:isolation & purification*; D011092:Polyethylene Glycols; D011506:Proteins / Q000302:isolation & purification*; D012965:Sodium Chloride; D013213:Starch / Q000031:analogs & derivatives*; D014867:Water
D007202:Indicators and Reagents; D009696:Nucleic Acids; D011506:Proteins; D014867:Water; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D013213:Starch; C046042:2-hydroxypropyl starch