ENU mutagenesis in the mouse electrophoretic specific-locus test, 1. Dose-response relationship of electrophoretically-detected mutations arising from mouse spermatogonia treated with ethylnitrosourea.
The mouse electrophoretic specific-locus test for induced germ-cell mutations, was used to determine the response of spermatogonial stem cells to a series of doses of the germ cell mutagen N-ethyl-N-nitrosourea (ENU). Male DBA/2J and C57B1/6J mice were treated with doses of 50, 100, 200 or 250 mg/kg ENU and their progeny screened for electrophoretically-detectable mutations at 32 separate loci. As expected, increasing doses of ENU led to increasing mutant frequencies. The differences in mutant frequencies between treated DBA/2J and C57B1/6J males were not statistically significant.
Lewis
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Research Triangle Institute, Research Triangle Park, NC 27709-2194.
Barnett
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Sadler
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Shelby
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MD
D000483:Alleles; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004592:Electrophoresis, Starch Gel; D005038:Ethylnitrosourea / Q000633:toxicity*; D005260:Female; D007668:Kidney; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D016296:Mutagenesis; D009152:Mutagenicity Tests; D013093:Spermatogonia / Q000187:drug effects*; D013234:Stem Cells
D005038:Ethylnitrosourea