Improved purification of leukocidin from Staphylococcus aureus and toxin distribution among hospital strains.
For purification of F and S components of the Panton-Valentine leukocidin, an easy three-step method using fast protein liquid chromatography was developed to replace the time-consuming purification procedures previously published. This technique enabled the recovery of 13 and 17 mg of purified F and S, respectively, per litre of culture supernatant. Affinity-purified neutralizing polyclonal antibodies were obtained against each individual component. One hundred and thirty-nine Staphylococcus aureus strains isolated from various clinical samples of hospitalized patients were screened by immunoprecipitation for Panton-Valentine leukocidin (PVL) production. Only 8 strains produced PVL; all originated from injured superficial soft tissues. Contrary to widespread opinion, the 8 PVL-producing strains were never associated with severe infection.
Finck-Barbançon
V
V
Institut de Bactériologie, Laboratoire de Toxinologie bactérienne, Strasbourg, France.
Prévost
G
G
Piémont
Y
Y
D000818:Animals; D006461:Hemolysis; D006801:Humans; D007956:Leukocidins / Q000302:isolation & purification* / Q000494:pharmacology / Q000502:physiology; D007962:Leukocytes / Q000187:drug effects; D008722:Methods; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000032:analysis* / Q000472:pathogenicity; D014774:Virulence
D007956:Leukocidins