title: A role for osteocalcin in osteoclast differentiation.
abstract: Specific cellular interactions with components of the extracellular matrix can influence cellular differentiation and development of many tissues. The extracellular matrix of bone is composed of organic constituents and a solid phase of calcium and inorganic phosphate (apatite). When implanted subcutaneously in rats, particles of bone matrix (BPs) recruit progenitors that differentiate into multinucleated cells with osteoclastic features. Because BPs deficient in osteocalcin, a bone matrix protein, were less efficient at promoting osteoclast formation than were normal BPs, we directly examined the influence of osteocalcin on osteoclast differentiation. We evaluated tissue responses to particles of synthetic crystalline apatite alone (Ap), having many of the features of native apatite of mature bone, or to apatite prepared with osteocalcin (Ap/OC), bovine serum albumin (Ap/BSA) or rat bone collagen (Ap/Col). Twelve days after subcutaneous implantation in normal rats, Ap, Ap/BSA, and Ap/Col particles generated a mild foreign body reaction with multinucleated cells in direct contact with the particles; these cells were negative for tartrate-resistant acid phosphatase (TRAP) activity and lacked ruffled borders. In contrast, Ap particles containing approximately 0.1% osteocalcin were partially resorbed and they generated more multinucleated cells that were TRAP-positive, were immunoreactive with an antibody against tartrate-resistant purple acid phosphatase, and displayed ultrastructural features of active osteoclasts including ruffled borders and clear zones. These data support the hypothesis that osteocalcin may function as a matrix signal in the recruitment and differentiation of bone-resorbing cells.
authors.affiliation: Department of Orthopedic Surgery, Brigham and Women's Hospital, Boston, Massachusetts 02115.
authors.forename: J
authors.initials: J
authors.lastname: Glowacki
authors.forename: C
authors.initials: C
authors.lastname: Rey
authors.forename: M J
authors.initials: MJ
authors.lastname: Glimcher
authors.forename: K A
authors.initials: KA
authors.lastname: Cox
authors.forename: J
authors.initials: J
authors.lastname: Lian
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D001031:Apatites / Q000494:pharmacology; D001862:Bone Resorption; D001842:Bone and Bones / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D002454:Cell Differentiation / Q000187:drug effects; D003094:Collagen / Q000494:pharmacology; D005109:Extracellular Matrix / Q000502:physiology; D005455:Fluorescent Antibody Technique; D008854:Microscopy, Electron; D015675:Osteocalcin / Q000494:pharmacology / Q000502:physiology*; D010010:Osteoclasts / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D051381:Rats; D013055:Spectrophotometry, Infrared
chemical_list: D001031:Apatites; D015675:Osteocalcin; D003094:Collagen; D000135:Acid Phosphatase