Effect of starvation and diabetes on the activity of the eukaryotic initiation factor eIF-2 in rat skeletal muscle.
The ability of the initiation factor eIF-2 in skeletal muscle extracts to form ternary initiation complexes ([Met-tRNA(f).eIF-2.GDP]) is decreased by either starvation or diabetes. These conditions also impair the ability of muscle extracts to dissociate [eIF-2.GDP], suggesting inhibition of the guanine nucleotide exchange reaction essential for eIF-2 recycling. We could not, however, detect any change in the phosphorylation state of the alpha subunit of eIF-2. This suggests that eIF-2 activity may be regulated in this system by a mechanism not involving its phosphorylation.
Jeffrey
I W
IW
Biochemistry Laboratory, School of Biological Sciences, University of Sussex, Falmer, Brighton, UK.
Kelly
F J
FJ
Duncan
R
R
Hershey
J W
JW
Pain
V M
VM
D000818:Animals; D003920:Diabetes Mellitus / Q000188:drug therapy / Q000378:metabolism; D015852:Eukaryotic Initiation Factor-2 / Q000096:biosynthesis* / Q000378:metabolism; D006150:Guanine Nucleotides / Q000378:metabolism; D006614:Hindlimb; D007328:Insulin / Q000008:administration & dosage; D009124:Muscle Proteins / Q000096:biosynthesis; D009132:Muscles / Q000378:metabolism*; D010766:Phosphorylation; D014176:Protein Biosynthesis / Q000502:physiology; D012358:RNA, Transfer, Met / Q000378:metabolism*; D051381:Rats; D013217:Starvation / Q000378:metabolism*
D015852:Eukaryotic Initiation Factor-2; D006150:Guanine Nucleotides; D007328:Insulin; D009124:Muscle Proteins; D012358:RNA, Transfer, Met