Detection and identification of Helicobacter pylori by the polymerase chain reaction.
A polymerase chain reaction for the specific detection of Helicobacter pylori was developed using a primer pair derived from the nucleotide sequence of the urease A gene of H pylori. Specific amplification of a 411 base pair DNA fragment from all strains of H pylori tested was achieved. Ten organisms were detected using the PCR and the technique permitted direct detection of H pylori in clinical biopsy samples. PCR will be useful for both prospective and retrospective investigation of the aetiology and epidemiology of H pylori associated disease.
Clayton
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C
Department of Medical Microbiology, St Bartholomew's Hospital Medical College, West Smithfield, London.
Kleanthous
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K
Tabaqchali
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S
D001483:Base Sequence; D004587:Electrophoresis, Agar Gel; D005753:Gastric Mucosa / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*