title: Dual effect of barium on basolateral membrane conductance of frog skin.
abstract: The effect of Ba2+ on basolateral membrane conductance (gi) in isolated frog skins was analysed. Response patterns were different in tissues with high and low spontaneous intracellular potential. At high (negative) potentials, serosal Ba2+ inhibited gi as is expected of a potent K+ channel blocker, whereas in tissues with low potential, gi remained unchanged or even increased after Ba2+. The direction of change in gi was also dependent on the magnitude of gi under control conditions. Decrease of gi was only observed at high gi in the control period. In contrast, gi increased if control values of gi were below 0.5 mS/cm2. In tissues with spontaneously low intracellular potential, an inhibitory effect of Ba2+ on gi could be induced by hyperpolarization of the basolateral membrane with transepithelial voltage perturbation. Under these conditions, voltage-dependent, inward rectifying K+ channels are activated, which are Ba2(+)-sensitive. Furthermore, hyperpolarization of the basolateral membrane potential (Vi) during Ba2+ rapidly decreased gi. These results suggest that Ba2+, in addition to blocking K+ channels, activates (presumably unspecific) basolateral membrane channels. This dual effect, which is obvious in tissues with low spontaneous gi, might similarly exist in tissues with high control gi. Identification, however, is virtually impossible due to the large decrease in potassium conductance.
authors.affiliation: Physiologisches Institut, Universität München, Federal Republic of Germany.
authors.forename: M
authors.initials: M
authors.lastname: Granitzer
authors.forename: W
authors.initials: W
authors.lastname: Nagel
mesh_terms: D000818:Animals; D001464:Barium / Q000494:pharmacology*; D004553:Electric Conductivity; D004594:Electrophysiology; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D015221:Potassium Channels / Q000187:drug effects; D011896:Rana temporaria; D012867:Skin / Q000187:drug effects / Q000378:metabolism*
chemical_list: D015221:Potassium Channels; D001464:Barium