Efflux of inorganic phosphate from rat hepatocytes: lack of effect of insulin.
Efflux of Pi from rat hepatocytes suspended in phosphate free-medium was studied by chemical assay of released Pi and by monitoring the loss in radioactivity of cells pre-labelled with [32P]-Pi. The release follows first-order kinetics fairly closely with a rate constant of 7 x 10(-3) min-1 approximately. Insulin at a concentration of 10(-8) M had no effect on the rate of Pi release and it is concluded therefore, that the insulin-stimulated accumulation of Pi described in the literature is the result of hormone action on Pi uptake by liver rather than on its release.
Parkash
M
M
Department of Biochemistry, King's College London, UK.
Butterworth
P J
PJ
D000818:Animals; D002478:Cells, Cultured; D007328:Insulin / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D010710:Phosphates