The use of irradiated embryos of Chrysomya bezziana for the detection of the Old World screw-worm fly.
Irradiation of Chrysomya bezziana embryos 1 h before hatching with doses less than or equal to 7 kilorad (kr) had a significant effect on percentage egg hatch, weights and survival of larvae. Doses greater than or equal to 1 kr allowed larval development to the end of the 3rd instar stage in vitro, but prevented normal pupal development. Cattle with wounds infested with 1st instar larvae derived from irradiated embryos had 3rd instar larvae present after 3 d but these failed to pupate. Thus it would be feasible to use such larvae for wound infestation for the enhanced detection of screw-worm fly in areas where the release of fertile flies is undesirable.
Clarke
G M
GM
DPIE/CSIRO Screw-Worm Fly Unit, Boroko, Papua New Guinea.
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D004175:Diptera / Q000254:growth & development / Q000528:radiation effects*; D007814:Larva / Q000254:growth & development / Q000528:radiation effects; D012610:Screw Worm Infection / Q000175:diagnosis / Q000662:veterinary*