Simplified procedure for transient transformation of plant protoplasts using polyethylene glycol treatment.
A modification of the polyethylene glycol-mediated transformation procedure which eliminates the manual polyethylene glycol dilution step is presented. A transformation mixture of protoplasts, DNA and polyethylene glycol was plated directly onto agarose blocks after incubation. The procedure was simple and fast, thereby suitable for screening the gene activity of large numbers of plasmid constructions. It has been tested for both maize and rice protoplasts.
Lyznik
L A
LA
Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907.
Peng
J Y
JY
Hodges
T K
TK
D004274:DNA, Recombinant; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D010944:Plants / Q000235:genetics*; D010957:Plasmids; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011523:Protoplasts; D014170:Transformation, Genetic / Q000187:drug effects*; D003313:Zea mays
D004274:DNA, Recombinant; D011092:Polyethylene Glycols; D005966:Glucuronidase