title: Physical mapping distal to the DMD locus.
abstract: We report a new locus, designated JC-1, which maps between the gene responsible for adrenal hypoplasia (AHC) and the gene that encodes glycerol kinase (GK) in Xp21.2-21.3. The probe identifying this locus was obtained by cloning the distal sequence of a junction fragment from a Duchenne muscular dystrophy (DMD) patient with a large deletion. Pulsed-field gel electrophoresis analysis shows that a region of at least 4 Mb separates the 3' end of the dystrophin gene and the closest distal marker to AHC, DXS28. This region of the human genome contains few genes whose deletion results in a clinical phenotype. JC-1 is a useful probe from which to initiate strategies directed at cloning the AHC and GK loci.
authors.affiliation: Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, England.
authors.forename: D R
authors.initials: DR
authors.lastname: Love
authors.forename: J F
authors.initials: JF
authors.lastname: Bloomfield
authors.forename: S J
authors.initials: SJ
authors.lastname: Kenwrick
authors.forename: J R
authors.initials: JR
authors.lastname: Yates
authors.forename: K E
authors.initials: KE
authors.lastname: Davies
mesh_terms: D015139:Blotting, Southern; D002460:Cell Line; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D005991:Glycerol Kinase / Q000235:genetics; D006801:Humans; D009136:Muscular Dystrophies / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D014770:Virilism / Q000235:genetics; D014960:X Chromosome
chemical_list: D004247:DNA; D005991:Glycerol Kinase