Molecular characterization of non-histone chromatin proteins from experimental tumours.
Using two-dimensional (2-D) electrophoresis, two non-histone chromatin protein fractions (NHCP1 and NHCP2) from three animal tumours (Kirkman-Robbins hepatoma, Morris hepatoma 7777 and Ehrlich ascites cells) and normal hamster liver were analyzed. Apart from many common components several tissue specific polypeptides of the NHCP1 and NHCP2 fractions were detected. It was found that some spots present in electropherograms of non-histone proteins of tumour cells (M X 10(-3)/pI): 17-24/4.9-6.5 (NHCP1 and NHCP2); 34-41/4.9-6.0 (HCP1 and NHCP2); 44-46/5.3-7.5 (HCP2); 46-49/5.0-7.5 (NHCP1); 49/5.9-7.5 (NHCP2) and 102-134/5.6-7.0 (NHCP1) were absent from normal liver.
Kiliańska
Z
Z
Department of Cytobiochemistry, Institute of Biochemistry, University of Lódź, Poland.
Krajewska
W M
WM
Lipińska
A
A
Marszałek
M
M
Gaczyński
M
M
Kłyszejko-Stefanowicz
L
L
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000737:chemistry; D002467:Cell Nucleus / Q000737:chemistry; D002843:Chromatin / Q000737:chemistry*; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry*; D006224:Cricetinae; D015180:Electrophoresis, Gel, Two-Dimensional; D008099:Liver / Q000737:chemistry; D008114:Liver Neoplasms, Experimental / Q000737:chemistry*; D051379:Mice; D008970:Molecular Weight; D009374:Neoplasms, Experimental / Q000737:chemistry*; D051381:Rats; D012394:Rosaniline Dyes; D012834:Silver
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D012394:Rosaniline Dyes; D012834:Silver; C048139:Coomassie blue