title: No evidence for the enhanced production of insulin autoantibodies after confrontation with common viral antigens in insulin dependent diabetes mellitus.
abstract: The production of insulin autoantibodies (IAA) was studied after common viral infections in 12 children with type 1 diabetes mellitus and in their 18 healthy siblings. In addition, the production of IAA was measured after influenza vaccination with booster in 39 patients with type 1 diabetes mellitus and in 39 healthy controls. In 7 of the 12 diabetic children 13 viral infections were serologically confirmed. Among the siblings 14 periods of infection were noted in 9 individuals. A significant rise in IAA antibody titre was demonstrated in patients twice (IgG both times) and in siblings 11 times (IgM 5x, IgG 6x, difference significant P less than 0.05). In only three cases the rise in antibody titres occurred 6-12 wk after documented infection. There was a significant inverse correlation with age in both patients (r = 0.89, P less than 0.0001) and siblings (r = 0.67, P less than 0.001) for IgM IAA. After influenza vaccination a significant increase in IAA was noted twice: IgM IAA in a patient with diabetes and IgG IAA in a healthy volunteer. A four-fold decrease in IgG IAA was demonstrated in one diabetic patient. From these results it is concluded that IAA formation is not a direct sequela of viral infection or vaccination.
authors.affiliation: Department of Virology, University Hospital Dijkzigt, Rotterdam.
authors.forename: R J
authors.initials: RJ
authors.lastname: Diepersloot
authors.forename: K P
authors.initials: KP
authors.lastname: Bouter
authors.forename: G J
authors.initials: GJ
authors.lastname: Bruining
authors.forename: J L
authors.initials: JL
authors.lastname: Molenaar
authors.forename: J B
authors.initials: JB
authors.lastname: Hoekstra
authors.forename: N
authors.initials: N
authors.lastname: Masurel
authors.forename: D W
authors.initials: DW
authors.lastname: Erkelens
mesh_terms: D000293:Adolescent; D000328:Adult; D001323:Autoantibodies / Q000032:analysis*; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000150:complications / Q000276:immunology*; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D007252:Influenza Vaccines / Q000276:immunology*; D007330:Insulin Antibodies / Q000032:analysis*; D008297:Male; D014777:Virus Diseases / Q000097:blood / Q000150:complications / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D007136:Immunoglobulins; D007252:Influenza Vaccines; D007330:Insulin Antibodies