Molecular cloning and sequence analysis of the gene encoding the major merozoite surface antigen of Plasmodium chabaudi chabaudi IP-PC1.
The complete nucleotide sequence of the gene encoding the precursor to the major merozoite surface antigens of Plasmodium chabaudi chabaudi strain IP-PC1 has been determined. A single open reading frame was detected, that coded for a protein of 199 kDa. The encoded protein (p199) contains putative signal and membrane anchor sequences and shows a clustering of Cys residues in the last 120 amino acids. Incompletely conserved tandem repeat oligopeptides are present at different positions in the molecule. P199 shows 69% overall homology to the analogous antigen in Plasmodium yoelii yoelii strain YM. The divergence between these antigens is largely confined to 4 areas where a number of insertions and/or deletions have occurred. All repeats occur in these divergent regions. The overall homology with both alleles of Plasmodium falciparum PMMSA is 33%.
Deleersnijder
W
W
Laboratorium Algemene Biologie, Instituut voor Moleculaire Biologie, Vrije Universiteit Brussel, Belgium.
Hendrix
D
D
Bendahman
N
N
Hanegreefs
J
J
Brijs
L
L
Hamers-Casterman
C
C
Hamers
R
R
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D020066:Merozoite Surface Protein 1; D008969:Molecular Sequence Data; D010961:Plasmodium / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*; D015800:Protozoan Proteins / Q000235:genetics*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D020066:Merozoite Surface Protein 1; D011498:Protein Precursors; D015800:Protozoan Proteins