title: Cloricromene inhibits the activation of human platelets by ADP alone or in combination with adrenaline.
abstract: Cloricromene may inhibit platelet activation induced by several agonists. In this study we report that ADP and adrenaline synergistically promote platelet aggregation and cytoplasmic Ca2+ movements in aequorin-loaded platelets. Cloricromene caused dose-dependent reduction in platelet aggregation and cytoplasmic Ca2+ movements after exposure of the cells to a low concentration of ADP (2 microM) or to a combination of ADP (2 microM) and adrenaline (10 microM). Cloricromene's inhibitory action may be of considerable pharmacological interest since platelet activation by a combination of agonists may mimic the conditions under which thrombosis occurs in vivo.
authors.affiliation: Laboratory of Vascular Biology, Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Del Maschio
authors.forename: G
authors.initials: G
authors.lastname: Bazzoni
authors.forename: A
authors.initials: A
authors.lastname: Zatta
authors.forename: Z M
authors.initials: ZM
authors.lastname: Chen
authors.forename: E
authors.initials: E
authors.lastname: Dejana
authors.forename: M
authors.initials: M
authors.lastname: Prosdocimi
mesh_terms: D000244:Adenosine Diphosphate / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D000331:Aequorin / Q000494:pharmacology; D002118:Calcium / Q000097:blood; D002866:Chromonar / Q000031:analogs & derivatives* / Q000494:pharmacology; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D004357:Drug Synergism; D004837:Epinephrine / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors
chemical_list: D010975:Platelet Aggregation Inhibitors; D000331:Aequorin; D000244:Adenosine Diphosphate; C025945:cloricromen; D002866:Chromonar; D002118:Calcium; D004837:Epinephrine