Candida albicans--adriamycin interactions: ultrastructural and spectrofluorometric study of whole yeasts and spheroplasts.
The occurrence of candidiasis in cancer patients who undergo chemotherapy requires the interrelation of Candida albicans and the antimitotic drug Adriamycin (ADM) which is well known as an intercalating agent. The whole yeasts were not affected by 2 h of contact with the drug at 10(-4) M neither for their growth curve nor for their ultrastructure, despite the presence of free ADM on their surface. Spheroplasts displayed a delay in their growth and exhibited altered nucleoli with segregation of their granular and fibrillar components. The modified emission spectrum of ADM, determined by spectrofluorometry, corresponded neither to the free ADM nor to the DNA-bound drug, but it could be related to a metabolite of the drug. The cell wall appeared to be one of the main sites for ADM resistance of Candida albicans in vitro.
Bobichon
H
H
UFR de Pharmacie 51, Reims, France.
Bussy
V
V
Angiboust
J F
JF
Manfait
M
M
Bouchet
P
P
Jardillier
J C
JC
D002176:Candida albicans / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D004317:Doxorubicin / Q000378:metabolism / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D008854:Microscopy, Electron; D013050:Spectrometry, Fluorescence; D013104:Spheroplasts / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure
D004317:Doxorubicin