Evaluation of DNA-binding activity of hydroxyanthraquinones occurring in Rubia tinctorum L.
The naturally-occurring anthraquinones (AQs), alizarin (1,2-dihydroxyanthraquinone) and lucidin (1,3-dihydroxy-2-hydroxymethylanthraquinone), were incubated with DNA in the presence of S9 mix. The isolated DNA was analysed by 32P-postlabelling for the presence of aromatic adducts. Only lucidin formed up to five different DNA adducts in the range from 0.995 to 3.05 adducts/10(8) nucleotides. Lucidin was also incubated with polynucleotides poly[d(A-T)] and polydG*polydC in the presence of S9 mix. Analysis of polydG*polydC revealed a similar adduct pattern to that obtained with lucidin-modified DNA. Alizarin, lucidin, a glycoside mixture containing alizarinprimeveroside and lucidinprimeveroside, and Rubia Teep (a herbal drug made from Rubia tinctorum containing lucidin) were incubated with primary rat hepatocytes for 24 h and the isolated DNA was analysed by 32P-postlabelling. Lucidin, the glycoside mixture and Rubia Teep gave rise to DNA adducts, but alizarin did not. Male Parkes mice were treated orally for 4 days with alizarin (10 mg/d), lucidin (2 mg/d), the glycoside mixture (20 mg/d) or Rubia Teep (1/2 tablet/d) and DNA was isolated from liver, kidney, duodenum and colon. Analysis by 32P-postlabelling revealed that lucidin, the glycoside mixture and Rubia Teep, but not alizarin, formed DNA adducts in all the tissues examined but that the adduct patterns were organ-specific.
Poginsky
B
B
Department of Toxicology, University of Hamburg Medical School, FRG.
Westendorf
J
J
Blömeke
B
B
Marquardt
H
H
Hewer
A
A
Grover
P L
PL
Phillips
D H
DH
D000818:Animals; D000880:Anthraquinones / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000378:metabolism; D010946:Plants, Medicinal / Q000032:analysis*
D000880:Anthraquinones; D010936:Plant Extracts; C010078:alizarin; C039315:lucidin; D004247:DNA