HLA class-II antigen-mediated induction of a proliferative response to anti-IgM in human B lymphocytes.
Signal transduction via HLA class-II antigens has been studied using human resting B lymphocytes and monoclonal anti-HLA class-II antibodies. An increased intracellular calcium flux, phosphatidylinositol biphosphate hydrolysis and activation of protein kinase C have all been observed following signal transduction via HLA-class-II molecules. The interaction of HLA-class-II-mediated signalling with sIg-mediated signalling has been studied using a non-mitogenic anti-sIg. This combination provides a model for T-cell-dependent antigenic stimulation. The results demonstrate that stimulation via HLA class-II antigens permits a proliferative response to an otherwise non-mitogenic anti-sIg and that this effect is not restricted to one HLA class-II isotype. These data suggest that HLA-class-II-mediated signalling is important in responses to T-cell-dependent antigens.
Mooney
N
N
Institut Biomédical des Cordeliers, Paris, France.
Van Alewyk
D
D
Brick-Ghannan
C
C
Charron
D
D
D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology*; D015153:Blotting, Western; D002478:Cells, Cultured; D004261:DNA Replication; D006681:HLA-D Antigens / Q000276:immunology*; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology*; D007700:Kinetics; D008213:Lymphocyte Activation; D013154:Spleen / Q000276:immunology
D000911:Antibodies, Monoclonal; D006681:HLA-D Antigens; D006684:HLA-DR Antigens; D007075:Immunoglobulin M