title: Density distribution and density conversion of neutrophils in allergic subjects.
abstract: We investigated the density distribution of neutrophils in peripheral blood of allergic subjects. We divided neutrophils into four groups: fraction 1 (density greater than 1.085), fraction 2 (1.081 less than density less than or equal to 1.085), fraction 3 (1.077 less than density less than or equal to 1.081) and fraction 4 (density less than or equal to 1.077). The percentage of neutrophils in fraction 2 in allergic rhinitis (AR) subjects or asthmatics was lower than that in normals (p less than 0.01). The percentage of neutrophils in fraction 3 and fraction 4 from AR or asthmatics was greater than that in normals (fraction 3, p less than 0.01; fraction 4, p less than 0.05). In neutrophils from AR subjects (fraction 3), chemotaxis to N-formyl-methionyl-leucyl-phenylalanine or platelet-activating factor (PAF) was enhanced compared to fraction 2. PAF (10(-7) M) changed the density of neutrophils (p less than 0.01), which were inhibited by WEB 2086 (p less than 0.05). Furthermore, granulocyte-macrophage colony-stimulating factor changed the density of neutrophils (p less than 0.01). These findings suggest that biological agents may activate neutrophils and convert their density resulting in neutrophils with lower density in allergic subjects.
authors.affiliation: Allergic Disease Center, Creighton University School of Medicine, Omaha, Nebr.
authors.forename: H
authors.initials: H
authors.lastname: Miyagawa
authors.forename: C
authors.initials: C
authors.lastname: Okada
authors.forename: H
authors.initials: H
authors.lastname: Sugiyama
authors.forename: R J
authors.initials: RJ
authors.lastname: Hopp
authors.forename: D K
authors.initials: DK
authors.lastname: Agrawal
authors.forename: M
authors.initials: M
authors.lastname: Nabe
authors.forename: C
authors.initials: C
authors.lastname: Gordy
authors.forename: A K
authors.initials: AK
authors.lastname: Bewtra
authors.forename: R G
authors.initials: RG
authors.lastname: Townley
mesh_terms: D001249:Asthma / Q000097:blood; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D002634:Chemotaxis, Leukocyte; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D006967:Hypersensitivity / Q000097:blood*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology* / Q000502:physiology; D010972:Platelet Activating Factor / Q000494:pharmacology
chemical_list: D010972:Platelet Activating Factor; D009240:N-Formylmethionine Leucyl-Phenylalanine; D016178:Granulocyte-Macrophage Colony-Stimulating Factor