title: Immunological studies on onchocerciasis in Sierra Leone. 1. Pretreatment baseline data.
abstract: Cellular immune responses were tested in vitro using peripheral blood mononuclear cells from 203 individuals resident in an area of Sierra Leone where onchocerciasis is hyperendemic, and 32 individuals (Gambians) with no history of contact with Onchocerca volvulus. Mean reactivity to the mitogen, Concanavalin A, did not differ between these two groups, but responses to PPD were markedly lower in those with onchocerciasis. Proliferative responses to adult female O. volvulus antigen in the latter group were generally low although elevated reactivity was found in certain sub-groups. Higher responses were evident in infected 10-14 year olds, and there was an association between elevated reactivity to O. volvulus antigens and acute reactive dermatological signs, with individuals in the latter group also carrying higher dermal microfilarial loads. A sub-group presenting with lymphadenopathy showed the strongest associations of these three parameters. These results suggested the requirement for a threshold density of dermal microfilariae for induction of acute reactivity. The presence of immunosuppressive factors in soluble O. volvulus antigen was indicated by the ability to suppress, at low concentrations, the cellular responses to PPD of a proportion of individuals.
authors.affiliation: MRC Laboratory, Bo, Sierra Leone.
authors.forename: A J
authors.initials: AJ
authors.lastname: Luty
authors.forename: M D
authors.initials: MD
authors.lastname: Downham
authors.forename: J A
authors.initials: JA
authors.lastname: Whitworth
authors.forename: D
authors.initials: D
authors.lastname: Morgan
authors.forename: A
authors.initials: A
authors.lastname: McNicholas
authors.forename: D W
authors.initials: DW
authors.lastname: Taylor
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003208:Concanavalin A / Q000276:immunology; D005260:Female; D005714:Gambia; D006801:Humans; D007111:Immunity, Cellular; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D009854:Onchocerca / Q000276:immunology; D009855:Onchocerciasis / Q000276:immunology*; D012189:Retrospective Studies; D012737:Sex Factors; D012807:Sierra Leone; D012867:Skin / Q000473:pathology; D014373:Tuberculin / Q000276:immunology
chemical_list: D000947:Antigens, Helminth; D014373:Tuberculin; D003208:Concanavalin A