title: [Spatial structure of the basic chain of the neurotoxin I molecule from the venom of cobra Naja naja oxiana and its crystal packing].
abstract: The structure of the alpha-carbon chain was solved by molecular replacement method at 2.7 A resolution. Neurotoxin I (NTX-I) is one of the main protein components purified from the venom of the central asian cobra Naja naja oxiana. NTX-I is known to bind specifically to acetylcholine receptors thus preventing the transmission of the neuroconductivity signal from synapses to muscles. NTX-I crystals were grown either by vapour diffusion or dialysis methods using specially prepared microdialysis cell. The intensities of reflections from native NTX-I crystals were measured in the range of 38.0-2.1 A-1 by omega-scan method with a Syntex P21 diffractometer operated in automatic regime. To determine the position and mode of packing of NTX-I molecule in unit cell program packages MERLOT and BLANC were applied running on a NORD-500 computer.
authors.forename: A M
authors.initials: AM
authors.lastname: Mikhaĭlov
authors.forename: A V
authors.initials: AV
authors.lastname: Nikitenko
authors.forename: E V
authors.initials: EV
authors.lastname: Chetverina
authors.forename: S D
authors.initials: SD
authors.lastname: Trakhanov
authors.forename: B K
authors.initials: BK
authors.lastname: Vaĭnshten
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002853:Chromatography, Liquid; D003460:Crystallization; D004546:Elapid Venoms / Q000737:chemistry*; D008969:Molecular Sequence Data; D009132:Muscles / Q000502:physiology; D009498:Neurotoxins / Q000737:chemistry*; D011487:Protein Conformation; D013569:Synapses / Q000502:physiology
chemical_list: D004546:Elapid Venoms; D009498:Neurotoxins
vernacular_title: Prostranstvennoe stroenie osnovnoĭ tsepi molekuly neĭrotoksina I iz iada kobry Naja naja oxiana i ee ukladka v kristallicheskoĭ iacheĭke.