Studies on the phenotype and karyotype of immortalized rabbit kidney epithelial cell lines.
Differentiated mammalian cell lines can be isolated by immortalizing primary cells by transfection with DNA from plasmids containing sequences from SV40 early region. These cell lines show cytogenetic abnormalities but the degree of aneuploidy is considerably less than that observed in other established cell lines. No correlation was observed between the degree of differentiation of a clone and the extent of chromosomal damage.
MacDonald
C
C
Department of Bioscience and Biotechnology, Todd Centre, Glasgow, Scotland.
Watts
P
P
Stuart
B
B
Kreuzburg-Duffy
U
U
Scott
D M
DM
Kinne
R K
RK
D000782:Aneuploidy; D000818:Animals; D001693:Biological Transport, Active; D002452:Cell Count; D002460:Cell Line; D002461:Cell Line, Transformed / Q000166:cytology* / Q000378:metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D007621:Karyotyping; D007684:Kidney Tubules / Q000166:cytology* / Q000378:metabolism; D010641:Phenotype; D011817:Rabbits; D014162:Transfection