[Increase in the fraction of non-repaired mutations in Escherichia coli cells, incubated in the absence of glucose after UV-irradiation].
In E. coli WP2 trpE65 cells irradiated with UV-dose of 11 J/m2, the additional small portion of induced Trp+ mutations became resistant to photoreactivation or "dark" (excision) repair after a short-termed (10-30 min) postirradiation incubation of bacteria in a minimal medium deprived of glucose and tryptophan. Since protein synthesis could not proceed in those cells because of the lack of energy and tryptophan, the data indicate that an unknown mechanism exists which imparts some mutations with the resistance to antimutagenic repair in the absence of the inducible mutagenic system. In the light of this result, one could suggest that the normal process of mutation fixation (that is the loss of sensitivity of mutations to photoreactivation or to excision repair in cells incubated in growth medium after irradiation) should not necessarily be a direct consequence of manifestation of the activity of an inducible mutagenic system.
Filippov
V D
VD
Lotareva
O V
OV
D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics* / Q000528:radiation effects; D005798:Genes, Bacterial; D005947:Glucose; D009154:Mutation; D011500:Protein Synthesis Inhibitors
D003470:Culture Media; D011500:Protein Synthesis Inhibitors; D005947:Glucose
Uvelichenie fraktsii nerepariruemykh mutatsiĭ v kletkakh Escherichia coli, proinkubirovannykh v otsutstvie gliukozy posle UF-oblucheniia.