title: Binding and internalization of immunoglobulin G by cultured human trophoblast.
abstract: A preparation consisting of a high percentage of trophoblasts can be obtained by centrifuging a mixture of cell types from trypsinized term placental villi on a discontinuous gradient of Percoll. When cultured these cells maintain trophoblast characteristics as judged by immunocytochemical markers and beta-human chorionic gonadotrophin production. Incubation of cells with labelled human immunoglobulin G demonstrated their ability to bind and internalize this macromolecule in a time- and temperature-dependent manner.
authors.affiliation: Department of Obstetrics and Gynaecology, Charing Cross and Westminster Medical School, West London Hospital, UK.
authors.forename: S R
authors.initials: SR
authors.lastname: Sooranna
authors.forename: S F
authors.initials: SF
authors.lastname: Contractor
mesh_terms: D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D007150:Immunohistochemistry; D013696:Temperature; D014327:Trophoblasts / Q000378:metabolism*
chemical_list: D007074:Immunoglobulin G