title: [Successful use of deep-frozen stallion sperm after 23 years of storage at -196 degrees C].
abstract: A reduction in the motility of the spermatozoa in stallion semen stored in pellet form for 23 years in liquid nitrogen at -196 degrees C could not be seen after thawing. The insemination of a mare with this semen resulted in a normal pregnancy. A normally developed, healthy male foal was born after a gestational period of 321 days.
authors.affiliation: Klinik für Andrologie und Besamung, Haustiere der Tierärztlichen Hochschule, Hannover.
authors.forename: H D
authors.initials: HD
authors.lastname: Krause
authors.forename: H
authors.initials: H
authors.lastname: Sieme
authors.forename: H
authors.initials: H
authors.lastname: Merkt
authors.forename: H
authors.initials: H
authors.lastname: Bader
authors.forename: A
authors.initials: A
authors.lastname: Wöckener
mesh_terms: D000818:Animals; D015925:Cryopreservation; D005260:Female; D005306:Fertilization; D006736:Horses / Q000502:physiology*; D008297:Male; D011247:Pregnancy; D011256:Pregnancy Outcome; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*
vernacular_title: Erfolgreicher Einsatz von tiefgefrorenem Hengstsperma nach 23jähriger Lagerung in Pellet-Form bei -196 degrees C.