title: Suppression of differentiation phenotypes in myogenic cells: association of aneuploidy and altered regulation of c-myc gene expression.
abstract: We isolated 10 myoblast clones from Syrian/golden hamster embryo (SHE) cells irradiated with ultraviolet light. They were originally isolated as anchorage-independent clones. All clones showed characteristic morphology of myoblast in culture and formed swirled myofiber colonies specific to myoblast macrocolonies. However, in immunostaining experiments using anti-fast myosin antibody, we found that 4 of 10 myoblast clones were myosin negative (Myo-), while six were myosin positive (Myo+). Western blot analysis confirmed the disappearance of the 200-kDa myosin-specific band in the 4 Myo- clones. Furthermore, these 4 Myo- clones lost the ability to form multinucleated myotubes. Karyotype analysis revealed that all Myo- clones had trisomy of chromosome 7, while Myo+ clones showed no apparent karyotypic change from normal SHE cells. Compared with that of SHE cells, the transcriptional level of the myc gene in Myo+ clones was augmented, but there was no increase of myc gene expression in Myo- clones. Furthermore, the introduction of activated myc gene partially converted the Myo- phenotype to Myo+. These results suggest that trisomy of chromosome 7 and a deficiency in enhanced expression of the myc oncogene are associated with the suppression of both the production of myosin and the formation of multinucleated cells.
authors.affiliation: Division of Radiation Biology, School of Medicine, Yokohama City University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: F
authors.initials: F
authors.lastname: Suzuki
authors.forename: O
authors.initials: O
authors.lastname: Nikaido
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
mesh_terms: D000782:Aneuploidy; D000818:Animals; D015153:Blotting, Western; D002448:Cell Adhesion; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D006224:Cricetinae; D015972:Gene Expression Regulation, Neoplastic; D016259:Genes, myc; D007621:Karyotyping; D008647:Mesocricetus; D009132:Muscles / Q000166:cytology; D010641:Phenotype