Avidin-peroxidase: synthesis and HPLC isolation of highly sensitive oligomers.
Optimal conditions for the preparation of avidin-peroxidase conjugates by the periodate method were studied. A method based on hydrophobic interaction chromatography was developed for the isolation of active oligomers. The probable structure of oligomers with highest sensitivity is discussed.
Gololobov
G V
GV
Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
Shuster
A M
AM
Zalite
I K
IK
Gabibov
A G
AG
Rabinkov
A G
AG
D001360:Avidin / Q000737:chemistry*; D001710:Biotin; D002851:Chromatography, High Pressure Liquid; D006735:Horseradish Peroxidase / Q000138:chemical synthesis / Q000737:chemistry*; D010084:Oxidation-Reduction; D011108:Polymers / Q000138:chemical synthesis / Q000302:isolation & purification
D011108:Polymers; D001360:Avidin; D001710:Biotin; D006735:Horseradish Peroxidase