A simple procedure for determination of the dead time of a stopped-flow instrument.
The reducing reaction of 2,6-dichlorophenolindophenol by L-ascorbic acid was used to determine the dead time of a stopped-flow instrument. Because this reaction is irreversible, the dead time could be determined by a simple graphical analysis. The dead time values determined by the present method were comparable to those by other methods previously reported.
Matsumura
K
K
Second Department of Physiology, Nara Medical University, Kashihara, Japan.
Enoki
Y
Y
Kohzuki
H
H
Sakata
S
S
D015086:2,6-Dichloroindophenol; D055598:Chemical Phenomena; D002621:Chemistry; D013053:Spectrophotometry / Q000379:methods*
D015086:2,6-Dichloroindophenol