title: Early events after intra-abdominal infection with Bacteroides fragilis and Escherichia coli.
abstract: Growth of Bacteroides fragilis and Escherichia coli was monitored during early stages of single (mono-) and mixed intra-abdominal infection in a rat fibrin clot model. When B. fragilis and E. coli were together involved in the infection, B. fragilis numbers increased about 6 h after an initial decline. This increase was not found with B. fragilis mono-infections. The numbers of E. coli increased rapidly in both mono- and mixed infections and stayed high for several days, but only mixed infection resulted in abscesses that persisted for more than 7 days. Macrophages, the main component of the peritoneal cellular defence mechanism, were outnumbered by polymorphonuclear leucocytes during the first 6 h of infection. Further characterisation of the macrophage population by means of monoclonal antibodies showed a shift from resident to exudate macrophages as the result of influx of the latter.
authors.affiliation: Department of Medical Microbiology, School of Medicine, Vrije Universiteit, Amsterdam, The Netherlands.
authors.forename: W R
authors.initials: WR
authors.lastname: Verweij
authors.forename: F
authors.initials: F
authors.lastname: Namavar
authors.forename: W F
authors.initials: WF
authors.lastname: Schouten
authors.forename: D M
authors.initials: DM
authors.lastname: MacLaren
mesh_terms: D000005:Abdomen; D000818:Animals; D001442:Bacteroides Infections / Q000150:complications* / Q000473:pathology; D001441:Bacteroides fragilis / Q000254:growth & development; D001777:Blood Coagulation; D002452:Cell Count; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000254:growth & development; D004927:Escherichia coli Infections / Q000150:complications* / Q000473:pathology; D005337:Fibrin; D006801:Humans; D008297:Male; D010529:Peritoneal Cavity; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D005337:Fibrin