title: Mesotocin in the brain and plasma of an Australian marsupial, the brushtail possum (Trichosurus vulpecula).
abstract: Oxytocic peptides extracted from the brain and plasma of the brushtail possum, Trichosurus vulpecula, were separated by reverse-phase high pressure lipid chromatography (HPLC) and quantified by specific radioimmunoassays for oxytocin (OT) and mesotocin (MT). The pituitary, hypothalamus and cerebral cortex were found to contain MT only in quantities of 3.9 +/- 0.2 (SE) ug, 17.6 +/- 0.6 ng and 21.0 +/- 2.6 ng respectively. The plasma concentration of MT varied according to the degree of stress of the possum. In anaesthetized animals values of 39.7 +/- 9.7 pg/ml (11 males) and 31.5 +/- 12.9 pg/ml (6 females) were obtained; in four conscious catheterized animals, 9.4 +/- 6.3 pg/ml. Samples taken from three anaesthetized animals during exsanguination contained 271 +/- 102 (SD) pg MT/ml. It was concluded that hypothalamic and extra-hypothalamic MT is present in the marsupial brain and that as in placental mammals, stress stimulates the secretion of mesotocin.
authors.affiliation: Department of Physiology and Pharmacology, University of Queensland, St Lucia, Australia.
authors.forename: R A
authors.initials: RA
authors.lastname: Bathgate
authors.forename: C
authors.initials: C
authors.lastname: Sernia
authors.forename: R T
authors.initials: RT
authors.lastname: Gemmell
mesh_terms: D000818:Animals; D001923:Brain Chemistry / Q000502:physiology*; D005260:Female; D008297:Male; D009893:Opossums / Q000097:blood / Q000378:metabolism*; D010121:Oxytocin / Q000031:analogs & derivatives* / Q000032:analysis; D011863:Radioimmunoassay; D012737:Sex Factors
chemical_list: C001713:mesotocin; D010121:Oxytocin; C008215:isotocin