title: Androstenedione and progesterone concentrations in preovulatory follicular fluid correlate with successful fertilization and cleavage of human oocytes in vitro.
abstract: OBJECTIVE
  To determine if androstenedione (A) and progesterone (P) concentrations in preovulatory follicular fluid (FF) correlate with successful fertilization and cleavage of human oocytes in vitro.
  
  
  METHODS
  A retrospective randomized trial.
  
  
  METHODS
  Hospital department of obstetrics and gynecology.
  
  
  METHODS
  Fifty-five patients, ages 24 to 39 years, with normal menstrual cycles undergoing in vitro fertilization (IVF) for tubal infertility.
  
  
  METHODS
  Multiple follicular development was induced with clomiphene citrate and human menopausal gonadotropin.
  
  
  METHODS
  Relationships among FF steroid hormone, morphological maturity of oocyte-corona-cumulus complexes, and fertilization and cleavage of oocytes.
  
  
  RESULTS
  Follicles with mature oocyte-corona-cumulus complexes and unfertilized oocytes contained significantly greater amounts of A (P less than 0.05) than those with mature oocyte-corona-cumulus complexes and fertilized ova, indicating the occurrence of atretic changes. Follicles yielding successfully fertilized and cleaved ova had significantly greater amounts of P (P less than 0.05) and A (P less than 0.01) but similar levels of 17 beta-estradiol compared with follicles yielding fertilized ova that failed to cleave.
  
  
  CONCLUSIONS
  Follicles yielding oocytes that cleaved as a result of IVF have both a shift in steroidogenesis from estrogen to progestin accumulation and declining aromatase activity, thus reflecting progressive luteinization of the follicles.
authors.affiliation: Tokyo Dental College, Ichikawa General Hospital, Chiba, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: T
authors.initials: T
authors.lastname: Oda
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshimura
authors.forename: Y
authors.initials: Y
authors.lastname: Takehara
authors.forename: M
authors.initials: M
authors.lastname: Natori
authors.forename: S
authors.initials: S
authors.lastname: Nozawa
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D000735:Androstenedione / Q000378:metabolism*; D002970:Cleavage Stage, Ovum; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000378:metabolism*; D005498:Follicular Phase; D006801:Humans; D008875:Middle Aged; D009865:Oocytes / Q000166:cytology*; D010062:Ovulation Induction / Q000379:methods; D011374:Progesterone / Q000378:metabolism*; D012189:Retrospective Studies
chemical_list: D000735:Androstenedione; D011374:Progesterone; D004958:Estradiol