Monitoring hepatitis B virus replication.
Serum hepatitis B virus DNA is the best marker for monitoring hepatitis B virus replication in the liver. Data are presented on the sensitivity, specificity and application of a standardized solution hybridization assay for the quantitation of hepatitis B viral DNA in serum. The assay is linear over several orders of magnitude in sample concentration and is equivalent in sensitivity and specificity to classic filter hybridization methods employing [32P]DNA probes and autoradiography. The assay provides clinically relevant information for monitoring viral replication before, during and after antiviral therapy.
Kuhns
M C
MC
Diagnostics Division, Abbott Laboratories, North Chicago, IL.
D004279:DNA, Viral / Q000097:blood / Q000302:isolation & purification*; D006509:Hepatitis B / Q000097:blood / Q000503:physiopathology* / Q000628:therapy; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D007370:Interferon Type I / Q000627:therapeutic use; D008099:Liver / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D011241:Prednisone / Q000627:therapeutic use; D011994:Recombinant Proteins
D004279:DNA, Viral; D007370:Interferon Type I; D011994:Recombinant Proteins; D011241:Prednisone