[The effect of pH, temperature and albumin content on the vitality of fetal rat liver cells during in vitro tests].
The influence of pH, temperature, and albumin content in the incubation medium on the vitality incubated hepatocytes was tested in vitro as well as the effects in the trypan-blue exclusion test. pH changes between 6.8 and 7.4 in the incubation medium have not any influence on the vitality of the hepatocytes or the trypan-blue exclusion test. Reduced vitality results occurred in an initial pH 7.8 in the incubation medium. The vital dye exclusion test is not useful in case of incubation temperature of 4 degrees C, since the results regarding the vitality of the hepatocytes are only apparently better. An addition of human serum albumin is necessary for a good survival of the hepatocytes during the incubation.
Hellmann
C
C
Klinik und Poliklinik für Kinderheilkunde, Bereiches Medizin (Charité), Humboldt-Universität zu Berlin.
Otting
U
U
Grossmann
P
P
D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000166:cytology* / Q000196:embryology; D051381:Rats; D012709:Serum Albumin / Q000378:metabolism*; D013696:Temperature; D014343:Trypan Blue
D003470:Culture Media; D012709:Serum Albumin; D014343:Trypan Blue
Einfluss von pH, Temperatur und Albumingehalt auf die Vitalität von Leberzellen fetaler Ratten bei in vitro-Versuchen.