[Design of recombinant-stable plasmids of the pFH series].
By cloning synthetic oligonucleotides into pUC18 plasmid, pFH123--pFH127 plasmids have been constructed. Their polylinker area, along with sites of widely used restriction endonucleases, contains two pairs each of FokI and HgaI sites in the opposite orientation to provide subfragment with unique predetermined 5'-ends. Comparative stability of the new plasmids and their derivatives has been studied and compared with that of the earlier constructed pMB plasmids.
Daniliuk
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NK
Serpinskiĭ
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OI
Siniakov
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D001483:Base Sequence; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids; D011995:Recombination, Genetic
Konstruirovanie rekombinantno-ustoĭchivykh plazmid serii pFH.