Resolution of the enantiomers of omeprazole and some of its analogues by liquid chromatography on a trisphenylcarbamoylcellulose-based stationary phase. The effect of the enantiomers of omeprazole on gastric glands.
The enantiomers of omeprazole and some of its analogues have been separated on a chiral stationary phase comprising trisphenylcarbamoylcellulose coated on 3-aminopropyl silica. The nature of the supporting silica has a crucial effect on the separations obtained. The racemisation half-life of omeprazole was estimated to be 1.3.10(2) h at 37 degrees C. In vitro tests on isolated gastric glands from rabbits showed that both enantiomers of omeprazole had an inhibitory effect on acid formation.
Erlandsson
P
P
Department of Technical Analytical Chemistry, University of Lund, Sweden.
Isaksson
R
R
Lorentzon
P
P
Lindberg
P
P
D000818:Animals; D002482:Cellulose / Q000031:analogs & derivatives*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005744:Gastric Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism; D006207:Half-Life; D009853:Omeprazole / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D010316:Particle Size; D011134:Polysaccharides / Q000737:chemistry; D011817:Rabbits; D013237:Stereoisomerism
D011134:Polysaccharides; D002482:Cellulose; C068231:trisphenylcarbamoylcellulose; D009853:Omeprazole