Determination of the specific activity of recombinant hirudin.
The anticipated importance of recombinant hirudin as an anti-thrombotic agent necessitates the development of tools for the quantification of its biological activity. A reproducible method for the determination of the specific activity of hirudin using a sensitive chromogenic assay is described. Purified recombinant hirudin proved to be close to 100% active versus thrombin. The method can also be used to determine the thrombin concentration if the hirudin concentration is known.
Eric
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Department of Molecular Biology, Transgene S.A., Strasbourg, France.
D000595:Amino Acid Sequence; D002863:Chromogenic Compounds / Q000378:metabolism; D006629:Hirudins / Q000494:pharmacology*; D007700:Kinetics; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D012044:Regression Analysis; D015203:Reproducibility of Results; D013696:Temperature; D013917:Thrombin / Q000037:antagonists & inhibitors* / Q000378:metabolism
D002863:Chromogenic Compounds; D006629:Hirudins; D009842:Oligopeptides; D011994:Recombinant Proteins; C034172:chromozym PL; D013917:Thrombin