title: Molecular characterization of non-histone chromatin proteins from experimental tumours.
abstract: Using two-dimensional (2-D) electrophoresis, two non-histone chromatin protein fractions (NHCP1 and NHCP2) from three animal tumours (Kirkman-Robbins hepatoma, Morris hepatoma 7777 and Ehrlich ascites cells) and normal hamster liver were analyzed. Apart from many common components several tissue specific polypeptides of the NHCP1 and NHCP2 fractions were detected. It was found that some spots present in electropherograms of non-histone proteins of tumour cells (M X 10(-3)/pI): 17-24/4.9-6.5 (NHCP1 and NHCP2); 34-41/4.9-6.0 (HCP1 and NHCP2); 44-46/5.3-7.5 (HCP2); 46-49/5.0-7.5 (NHCP1); 49/5.9-7.5 (NHCP2) and 102-134/5.6-7.0 (NHCP1) were absent from normal liver.
authors.affiliation: Department of Cytobiochemistry, Institute of Biochemistry, University of Lódź, Poland.
authors.forename: Z
authors.initials: Z
authors.lastname: Kiliańska
authors.forename: W M
authors.initials: WM
authors.lastname: Krajewska
authors.forename: A
authors.initials: A
authors.lastname: Lipińska
authors.forename: M
authors.initials: M
authors.lastname: Marszałek
authors.forename: M
authors.initials: M
authors.lastname: Gaczyński
authors.forename: L
authors.initials: L
authors.lastname: Kłyszejko-Stefanowicz
mesh_terms: D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000737:chemistry; D002467:Cell Nucleus / Q000737:chemistry; D002843:Chromatin / Q000737:chemistry*; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry*; D006224:Cricetinae; D015180:Electrophoresis, Gel, Two-Dimensional; D008099:Liver / Q000737:chemistry; D008114:Liver Neoplasms, Experimental / Q000737:chemistry*; D051379:Mice; D008970:Molecular Weight; D009374:Neoplasms, Experimental / Q000737:chemistry*; D051381:Rats; D012394:Rosaniline Dyes; D012834:Silver
chemical_list: D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D012394:Rosaniline Dyes; D012834:Silver; C048139:Coomassie blue