[Growth of Coxiella burnetii in selected cell cultures].
Usefulness of monolayer cell cultures for C. burnetii multiplication was evaluated. Continuous Vero cell line and primary cell (cultures cell culture of monkey kidney Cercopithecus aethiops, cell culture of chicken fibroblasts, diploid cell line of human embryo) was inoculated for this purpose. Two Coxiella burnetii strains suspensions were used as inoculum of standard Henzerling strain and Zamość strain isolated from an area of Poland. Primary cell lines were characterized by considerable degree of sensitivity to, C. burnetii infection, and at the same time, the most sensitive was the line derived from monkey kidney. Progressive vacuolisation of infected culture cells was designated as cytopathic effect. Henzerling strain showed higher infectivity (4.7 x 10(8) PFU/ml) for chicken fibroblasts as compared to Zamość strain (8.7 x 10(6) PFU/ml). On the other hand both strains induced cytopathic effect in Vero cell line without degeneration of host cells.
Rumin
W
W
Zakład Bakteriologii PZH w Warszawie.
Kruszewska
D
D
Sadowski
W
W
Tylewska-Wierzbanowska
S
S
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D015169:Colony Count, Microbial; D004622:Embryo, Mammalian; D005347:Fibroblasts; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney; D007709:Klebsiella / Q000254:growth & development*
Wzrost Coxiella burnetii w wybranych hodowlach komórkowych.