Direct sequencing of PCR products using the Maxam-Gilbert method.
Direct sequencing of polymerase chain reaction (PCR) products by using the Maxam-Gilbert method is described. In this method, one of the primers is end labeled. Thus it is possible to sequence the reaction product directly following purification using this chemical method.
Stamm
S
S
Fishberg Research Center for Neurobiology, Mount Sinai School of Medicine, New York, NY 10029.
Longo
F M
FM
D001483:Base Sequence; D009710:Nucleotide Mapping / Q000379:methods; D016133:Polymerase Chain Reaction