Monocarboxylate carrier from bovine heart mitochondria: reconstitution in a functionally active state.
A method has been developed for functional reconstitution of the isolated pyruvate carrier. Optimal conditions were reached after removal of deoxycholate with 27 mg Triton X-100 per gram of moist Amberlite XAD-2 beads and 7 micrograms protein/mg of phospholipid. Uptake of pyruvate into proteoliposomes and sensitivity of the carrier towards inhibitors resemble those in intact mitochondria.
Nałecz
K A
KA
Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, Warszawa, Poland.
Zambrowicz
B E
BE
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002417:Cattle; D002852:Chromatography, Ion Exchange; D003902:Detergents; D007700:Kinetics; D008929:Mitochondria, Heart / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism*
D002352:Carrier Proteins; D003902:Detergents; D011773:Pyruvates