Construction and regional localization of clones from a NotI linking library from human chromosome 17q.
A NotI linking library was constructed from a somatic cell hybrid containing chromosome 17q as its only human material. A total of 112 human clones were assigned to nine regions of 17q using a somatic cell hybrid mapping panel. The library includes clones that detect the acute promyelocytic leukemia and von Recklinghausen neurofibromatosis translocation breakpoints at 17q11.2-12 and 17q11.2, respectively, on pulsed-field gel electrophoresis. The mapped clones represent over 50% of the estimated number of NotI sites on 17q, and therefore constitute an important resource for long-distance mapping.
Borrow
J
J
Somatic Cell Genetics Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
Black
D M
DM
Goddard
A D
AD
Yagle
M K
MK
Frischauf
A M
AM
Solomon
E
E
D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D003001:Cloning, Molecular; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D015723:Gene Library; D008040:Genetic Linkage; D006801:Humans
D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases