Anti-thymocyte globulin induced thrombocytopenia.
Anti-thymocyte globulin is strongly reactive with platelets as measured by flow cytometric analysis. This reactivity appears to be independent of human leukocyte antigen (HLA) phenotype, is dose-dependent with saturation of platelet binding sites readily achieved with concentrations of horse anti-thymocyte globulin (ATG) at 0.25 mg per ml, and cannot be blocked with human anti-PLA1 antibody nor with heat-aggregated human IgG. Results of immunoblot studies after electrophoresis of platelet membrane proteins are consistent with the proposal that horse ATG contains antibodies specific for platelet glycoproteins V and IIa.
Lazarchick
J
J
Department of Pathology/Laboratory Medicine, Medical University of South Carolina, Charleston 29425.
Russell
R
R
Horn
B
B
D000918:Antibody Specificity; D001792:Blood Platelets / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D006680:HLA Antigens / Q000276:immunology; D006358:Hot Temperature; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000276:immunology; D007518:Isoantibodies / Q000009:adverse effects* / Q000276:immunology; D008970:Molecular Weight; D010980:Platelet Membrane Glycoproteins / Q000276:immunology; D011961:Receptors, Fc / Q000276:immunology; D013921:Thrombocytopenia / Q000209:etiology*
D006680:HLA Antigens; D007074:Immunoglobulin G; D007518:Isoantibodies; D010980:Platelet Membrane Glycoproteins; D011961:Receptors, Fc; C035678:anti-Thy antibody