Fibril amyloid enhancing factor (FAEF)-accelerated amyloidosis in the hamster is not dependent on serine esterase activity and mononuclear phagocytosis.
Cells of the mononuclear phagocytic system (MPS) were described as playing a decisive role in amyloidogenesis. A relationship between the amyloid enhancing factor (AEF) and MPS cells was suggested and recently AEF activity was attributed to a serine esterase (SE) of leucocytic origin. In the present study, no correlation was found between the SE content and AEF activity in either peritoneal cell lysates or AEF preparations of different origin. Furthermore, pretreatment of fibril AEF (FAEF) with the SE inhibitor phenylmethylsulphonyl fluoride (PMSF) did not affect its activity in the hamster. Blockade of the MPS by dextran sulphate did not inhibit deposition of amyloid after intravenous injection of FAEF but amyloid deposition was inhibited when FAEF was administered intraperitoneally. These results suggest that MPS cells could be involved in transport of AEF, but that phagocytic activity of MPS cells is not essential in AA-amyloid fibrillogenesis. It is concluded that these results are not consistent with the previously suggested nature of the AEF or with the proposed central role of the MPS in amyloidogenesis.
Niewold
T A
TA
Department of Veterinary Pathology, University of Utrecht, The Netherlands.
Gruys
E
E
Kisilevsky
R
R
Shirahama
T S
TS
D000686:Amyloidosis / Q000139:chemically induced / Q000378:metabolism*; D000818:Animals; D006224:Cricetinae; D016264:Dextran Sulfate / Q000494:pharmacology; D004195:Disease Models, Animal; D004950:Esterases / Q000378:metabolism*; D005260:Female; D006023:Glycoproteins / Q000008:administration & dosage / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000201:enzymology*; D008297:Male; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology; D010587:Phagocytosis / Q000187:drug effects; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology
D006023:Glycoproteins; C046748:amyloid enhancing factor; D010664:Phenylmethylsulfonyl Fluoride; D016264:Dextran Sulfate; D004950:Esterases; C022635:serine esterase