title: One-electron reduction of daunorubicin intercalated in DNA or in a protein: a gamma radiolysis study.
abstract: The one-electron reduction of daunorubicin, an anthracycline antibiotic, intercalated in DNA or in the apoprotein of the riboflavin binding protein, was studied by gamma radiolysis. The two reduction mechanisms appear very similar to the one found for the non-intercalated drug. Hydrogen peroxide, which oxidizes non-intercalated hydroquinone daunorubicin with two electrons in one step (C. Houée-Levin, M. Gardès-Albert and C. Ferradin, FEBS lett., 173, 27-30, (1984], reacts with daunorubicin hydroquinone in DNA but not in the protein. It appears thus that the site accessibility to hydrogen peroxide in DNA is better than in the protein. Biological consequences are discussed.
authors.affiliation: Laboratoire de Chimie Physique, Université Paris V, France.
authors.forename: C
authors.initials: C
authors.lastname: Houée-Levin
authors.forename: M
authors.initials: M
authors.lastname: Gardès-Albert
authors.forename: K
authors.initials: K
authors.lastname: Benzineb
authors.forename: A
authors.initials: A
authors.lastname: Rouscilles
authors.forename: C
authors.initials: C
authors.lastname: Ferradini
mesh_terms: D001059:Apoproteins / Q000187:drug effects* / Q000378:metabolism; D002352:Carrier Proteins / Q000187:drug effects* / Q000378:metabolism; D004247:DNA / Q000187:drug effects*; D003630:Daunorubicin / Q000378:metabolism / Q000528:radiation effects*; D005720:Gamma Rays; D006861:Hydrogen Peroxide / Q000378:metabolism; D007364:Intercalating Agents / Q000378:metabolism / Q000528:radiation effects*; D026901:Membrane Transport Proteins; D008956:Models, Chemical; D010084:Oxidation-Reduction; D010782:Photolysis; D013329:Structure-Activity Relationship
chemical_list: D001059:Apoproteins; D002352:Carrier Proteins; D007364:Intercalating Agents; D026901:Membrane Transport Proteins; C019079:riboflavin-binding protein; D004247:DNA; D006861:Hydrogen Peroxide; D003630:Daunorubicin