title: Autoradiographic and functional development of gastric cholecystokinin receptors in the rat.
abstract: To determine the functional role for and the pharmacological specificity of developing gastrointestinal CCK receptors, in vitro pyloric contractility and autoradiographic CCK receptor binding were examined in pups aged 1-20 days. CCK contracted the gastroduodenal junction at all ages, while nonsulfated CCK-8 (d-CCK) was less potent. Autoradiographic studies revealed CCK binding localized to the gastroduodenal junction throughout development. MK-329, a specific type A CCK receptor antagonist, completely displaced 125I CCK-8 binding at all ages, while d-CCK displaced binding at ages at which d-CCK elicited gastroduodenal contractility. The results demonstrate a physiological role for and pharmacological specificity of neonatal gastroduodenal CCK receptors.
authors.affiliation: Department of Psychiatry and Behavioral Sciences Johns Hopkins University School of Medicine, Baltimore, MD 21205.
authors.forename: G J
authors.initials: GJ
authors.lastname: Schwartz
authors.forename: T H
authors.initials: TH
authors.lastname: Moran
authors.forename: P R
authors.initials: PR
authors.lastname: McHugh
mesh_terms: D000818:Animals; D001345:Autoradiography; D005753:Gastric Mucosa / Q000378:metabolism*; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D011706:Pyloric Antrum; D051381:Rats; D011919:Rats, Inbred Strains; D011949:Receptors, Cholecystokinin / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D013270:Stomach / Q000254:growth & development
chemical_list: D011949:Receptors, Cholecystokinin