Immunocytochemical localization of amelogenin in rat incisor ameloblasts using ultrathin frozen sections.
The localization of amelogenin, an enamel matrix protein, was examined by ultrastructural immunocytochemistry using unembedded ultrathin frozen sections of undecalcified rat incisor ameloblasts. Antibody against bovine amelogenin labeled Golgi complexes, secretory granules, and lysosomal structures in the preameloblasts and inner enamel-secretory ameloblasts as well as the enamel. The antibody also labeled dentin matrix facing preameloblasts. These results support the findings in previous reports using conventional epon embedded specimens. However, rough-surfaced endoplasmic reticulum failed to be labeled by this antibody.
Nishikawa
S
S
Department of Histology, Kanagawa Dental College, Japan.
Takagi
T
T
Sasa
S
S
D000565:Ameloblasts / Q000648:ultrastructure*; D053523:Amelogenin; D000818:Animals; D003746:Dental Enamel Proteins / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D007180:Incisor / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains
D053523:Amelogenin; C507201:Amelx protein, rat; D003746:Dental Enamel Proteins