An enzymatic method for detecting MAO-A and MAO-B inhibitors in plasma and its application in studies with reversible MAO-A selective inhibitors.
A general method was developed for the simultaneous enzymatic assay of MAO-A and MAO-B inhibitors in plasma of pretreated animals. After extraction of the inhibitor from plasma into an appropriate volatile organic solvent, dried aliquots of the organic layer are incubated with a buffered rat brain MAO preparation and substrates, [3H]serotonin and [14C]phenethylamine. Under conditions employed, the presence in plasma of MAO-A and/or B inhibitors can be detected.
White
H L
HL
Division of Pharmacology, Wellcome Research Laboratories, Research Triangle Park, N.C.
D000818:Animals; D001921:Brain / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008995:Monoamine Oxidase / Q000378:metabolism; D008996:Monoamine Oxidase Inhibitors / Q000097:blood*; D010627:Phenethylamines / Q000378:metabolism; D051381:Rats; D012701:Serotonin / Q000378:metabolism; D013892:Thioxanthenes / Q000494:pharmacology
D008996:Monoamine Oxidase Inhibitors; D010627:Phenethylamines; D013892:Thioxanthenes; D012701:Serotonin; C068591:BW 616U76; D008995:Monoamine Oxidase