On the degradation and elimination of spermine by the vertebrate organism.
1. Treatment of mice and rats with the polyamine oxidase inhibitor N1,N4-bis-(2,3-butadienyl)-1,4-butanediamine (MDL 72527) causes a gradual accumulation of spermine in the circulation and a decrease of spermidine concentration. 2. Spermine is mainly localized in the red blood cells. 3. Co-administration of 2-(difluoromethyl)ornithine and MDL 72527 enhances considerably the rate and extent of spermine accumulation in the circulation. 4. It is assumed that the increased rate of spermine accumulation by the two drugs is due to the enhancement of cell death, i.e. spermine accumulation is the result of its release into the circulation from dying cells, not due to physiological release. 5. After discontinuation of polyamine oxidase inhibition spermine appears to be gradually transformed into spermidine by red blood cell polyamine oxidase, obviously without transformation into N1-acetylspermine.
Sarhan
S
S
Merrell Dow Research Institute, Strasbourg, France.
Quemener
V
V
Moulinoux
J P
JP
Knödgen
B
B
Seiler
N
N
D000818:Animals; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D007700:Kinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011073:Polyamines / Q000097:blood / Q000652:urine; D011700:Putrescine / Q000031:analogs & derivatives; D051381:Rats; D011919:Rats, Inbred Strains; D013095:Spermidine / Q000378:metabolism*
D011073:Polyamines; C044445:MDL 72527; D013095:Spermidine; D011700:Putrescine