Centromeric DNA of Kluyveromyces lactis.
A direct selection method was used to isolate centromeres from a genomic library of the yeast Kluyveromyces lactis. The method is based on the lethality at high copy number of the ochre-suppressing tRNA gene SUP11. Five different chromosomal fragments were found that confer mitotic stability to plasmids containing a replication origin of K. lactis (KARS). In addition, KARS plasmids containing these fragments have a copy number of approximately one, and each of the five fragments hybridizes to a different chromosome of K. lactis. From these results we conclude that five of the six centromeres of K. lactis have been isolated. These centromeres do not function in S. cerevisiae.
Heus
J J
JJ
Department of Cell Biology and Genetics, Leiden University, The Netherlands.
Zonneveld
B J
BJ
Steensma
H Y
HY
Van den Berg
J A
JA
D002503:Centromere; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000302:isolation & purification; D005800:Genes, Fungal; D015698:Genomic Library; D007716:Kluyveromyces / Q000235:genetics*; D008938:Mitosis; D010957:Plasmids; D015183:Restriction Mapping
D004271:DNA, Fungal