[17 Beta-estradiol interaction with haptoglobin and its complex with hemoglobin].
The estradiol-binding capacity of sheep haptoglobin and its complex with hemoglobin was investigated. It was shown that in the presence of H2O2 the haptoglobin-hemoglobin complex tightly binds 17 beta-estradiol. No dissociation of the estradiol-protein complex occurs after its precipitation with trichloroacetic acid and steroid extraction with the ester. It is supposed that the 17 beta-estradiol is bound to the protein by covalent bonds; this binding is characteristic only of the haptoglobin-hemoglobin complex but not of haptoglobin alone.
Isaeva
G M
GM
Smirnov
A N
AN
Rozen
V B
VB
Beĭsembaeva
D000818:Animals; D004958:Estradiol / Q000378:metabolism*; D006242:Haptoglobins / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism*; D006861:Hydrogen Peroxide; D011485:Protein Binding; D012756:Sheep; D014238:Trichloroacetic Acid
D006242:Haptoglobins; D006454:Hemoglobins; D004958:Estradiol; D014238:Trichloroacetic Acid; D006861:Hydrogen Peroxide
Issledovanie vzaimodeĭstviia 17 beta-éstradiola s gaptoglobinom i egokompleksom s gemoglobinom.