title: Localization of platelet-derived endothelial cell growth factor in human placenta and purification of an alternatively processed form.
abstract: Platelet-derived endothelial cell growth factor (PD-ECGF) was purified to homogeneity from human term placenta, an organ characterized by extensive angiogenesis. N-terminal amino acid sequencing revealed that placental PD-ECGF was proteolytically processed at Thr-6, in contrast to PD-ECGF purified from human platelets, which is processed at Ala-11. The purified factor stimulated porcine aortic endothelial cells as well as two choriocarcinoma cell lines. Immunohistochemical staining revealed that PD-ECGF was present in the connective tissue cells of the placenta. The possibility that PD-ECGF is involved in the development of the placenta is discussed.
authors.affiliation: Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden.
authors.forename: K
authors.initials: K
authors.lastname: Usuki
authors.forename: L
authors.initials: L
authors.lastname: Norberg
authors.forename: E
authors.initials: E
authors.lastname: Larsson
authors.forename: K
authors.initials: K
authors.lastname: Miyazono
authors.forename: U
authors.initials: U
authors.lastname: Hellman
authors.forename: C
authors.initials: C
authors.lastname: Wernstedt
authors.forename: K
authors.initials: K
authors.lastname: Rubin
authors.forename: C H
authors.initials: CH
authors.lastname: Heldin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004727:Endothelium / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D010920:Placenta / Q000737:chemistry* / Q000502:physiology; D010982:Platelet-Derived Growth Factor / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology; D011499:Protein Processing, Post-Translational; D013552:Swine; D014407:Tumor Cells, Cultured
chemical_list: D010982:Platelet-Derived Growth Factor