title: Serum, trypsin, and cell shape but not cell-to-cell contact influence the X-ray sensitivity of Chinese hamster V79 cells in monolayers and in spheroids.
abstract: Nutrient concentration in the growth medium and trypsin affect cellular radiosensitivity in a manner that is related to cell shape (Reddy, Stevenson, and Lange, Int. J. Radiat. Biol. 55, 105-117 (1989); Reddy and Lange, Radiat. Res. 119, 338-347 (1989]. Hence we hypothesized that the concentration of serum in the medium could influence the X-ray sensitivity of cells and that the spread cells in monolayers and round cells in spheroids may differ in their response to the radiosensitizing effect of trypsin. We compared the X-ray sensitivity of monolayer and spheroid cells grown for 19 +/- 1 h in MEM supplemented with 5 or 15% serum. Cells were trypsinized and plated either immediately before, or 2.5 +/- 0.5 h after, irradiation and incubation for repair in situ. Survival of cells in monolayers and in spheroids was higher in MEM with 5% serum than with 15% serum. Trypsin treatment affected the shape and radiosensitivity of cells in monolayers but not in spheroids. When all cells were grown in the same serum concentration and a 2.5-h postirradiation incubation was allowed prior to trypsinization, the X-ray sensitivity of cells in spheroids was greater than that of cells in monolayers. The survival of cells in spheroids became equal to that of monolayer cells when cells in spheroids were converted to monolayers by placing them in 25-cm2 flasks and allowing them 3 h to attach and spread. Cell cycle distributions were nearly the same in monolayers and spheroids cultured in MEM with 5 or 15% serum. We conclude that: (1) serum concentration in the growth medium and trypsin do appear to contribute to the differences in the radiosensitivity of spheroids and monolayer V79 cells; (2) these differences are associated with changes in cell morphology.
authors.affiliation: Department of Radiation Oncology, SUNY Health Science Center at Brooklyn, Brooklyn 11203.
authors.forename: N M
authors.initials: NM
authors.lastname: Reddy
authors.forename: C S
authors.initials: CS
authors.lastname: Lange
mesh_terms: D000818:Animals; D001769:Blood; D002449:Cell Aggregation / Q000502:physiology; D002450:Cell Communication / Q000502:physiology; D002470:Cell Survival / Q000528:radiation effects*; D003470:Culture Media; D066298:In Vitro Techniques; D011836:Radiation Tolerance / Q000187:drug effects / Q000502:physiology*; D014357:Trypsin / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D014357:Trypsin