Application of sodium lauryl sulphate ion-pairing reversed-phase high-performance liquid chromatography in the separation of some isomeric and other closely related basic compounds in pharmaceutical analysis.
Ion-pair reversed-phase high-performance liquid chromatography proved to be useful for the separation of basic compounds of similar structure. Using suitable ion-pairing reagent concentration, pH and ionic strength values, good separations can be achieved. An ion-pair chromatographic study of a benzodioxanyl derivative, an aminopyrimidine derivative and a lysergic acid derivative was performed. Each of these compounds may be contaminated by a structural isomer produced as a by-product during the synthesis. By choosing the optimum experimental conditions these isomers can be separated from each other and from other related compounds and can be quantified in various substances and pharmaceutical products.
Bidló-Iglóy
M
M
Institute for Drug Research, Budapest, Hungary.
D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004146:Dioxanes / Q000032:analysis* / Q000378:metabolism; D007536:Isomerism; D008237:Lysergic Acid / Q000032:analysis* / Q000378:metabolism; D011743:Pyrimidines / Q000032:analysis* / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate
D004146:Dioxanes; D011743:Pyrimidines; D012967:Sodium Dodecyl Sulfate; D008237:Lysergic Acid