title: Distribution of neuropeptide Y and vasoactive intestinal polypeptide immunoreactive nerves in normal and transplanted pancreatic tissue.
abstract: Neuropeptide Y (NPY) and vasoactive intestinal polypeptide (VIP) immunoreactive nerves were demonstrated in 21-day-old embryonic pancreatic tissue fragments transplanted into the anterior eye chamber of rats for 22, 45 and 109 days and in 60-day-old normal adult pancreas using immunohistochemical technique. In normal adult tissue, NPY-positive neurons lie close to the basal and lateral walls of the acinar cells. NPY-containing nerve fiber plexuses were found around blood vessels. VIP-immunopositive nerves were also discernible in the outer parts of the islets of Langerhans and on pancreatic ducts. In the transplants, it is not only the neural elements that survived but also the pancreatic ducts and the endocrine cells. VIP- and NPY-positive neurons were found in the stroma of the surviving pancreatic tissue. The distribution of these neural elements is similar to that of normal tissue in the surviving pancreatic ducts but different with regards to the acinar tissue. This study confirms that intrinsic nerves can survive and synthesize polypeptides even after 109 days of transplantation into the anterior eye chamber.
authors.affiliation: 1st Department of Anatomy, Histology and Embryology, Semmelweis University Medical School, Budapest, Hungary.
authors.forename: E
authors.initials: E
authors.lastname: Adeghate
authors.forename: T
authors.initials: T
authors.lastname: Donáth
mesh_terms: D000818:Animals; D000867:Anterior Chamber / Q000601:surgery; D016332:Fetal Tissue Transplantation / Q000473:pathology / Q000502:physiology*; D007124:Immunoenzyme Techniques; D009478:Neuropeptide Y / Q000032:analysis*; D010179:Pancreas / Q000737:chemistry / Q000196:embryology / Q000294:innervation*; D016035:Pancreas Transplantation / Q000473:pathology / Q000502:physiology*; D051381:Rats; D012016:Reference Values; D016042:Transplantation, Heterotopic / Q000502:physiology; D014660:Vasoactive Intestinal Peptide / Q000032:analysis*
chemical_list: D009478:Neuropeptide Y; D014660:Vasoactive Intestinal Peptide