A new method for the purification of the influenza A virus neuraminidase.
A rapid new method for the purification of neuraminidase (NA) heads from influenza A virus is described. Virus was pelleted directly from allantoic fluid and was digested with pronase. The cores were removed by centrifugation, redigested and the released NA heads were pooled and concentrated. The NA was separated from all contaminating proteins in a single step on a Superose 12 column. The purified material was suitable for both crystallography and for the production of monospecific antisera.
McKimm-Breschkin
J L
JL
CSIRO Division of Biomolecular Engineering, Parkville, Australia.
Caldwell
J B
JB
Guthrie
R E
RE
Kortt
A A
AA
D000818:Animals; D002498:Centrifugation; D002642:Chick Embryo; D002850:Chromatography, Gel; D009980:Influenza A virus / Q000201:enzymology*; D009439:Neuraminidase / Q000302:isolation & purification*; D011402:Pronase / Q000378:metabolism
D009439:Neuraminidase; D011402:Pronase