Induction of experimental systemic lupus erythematosus in mice by immunization with a monoclonal anti-La autoantibody.
Experimental systemic lupus erythematosus (SLE) in mice can be induced by immunization either with a human monoclonal anti-DNA antibody bearing the 16/6 idiotype (16/6 Id) or with a mouse monoclonal anti-idiotypic antibody specific for the 16/6 Id. In the present report we investigated the pathogenic role of a monoclonal anti-La autoantibody in the induction and mediation of experimental SLE in mice. The monoclonal anti-La antibody was derived from a mouse in which experimental SLE was induced by immunization with the monoclonal anti-16/6 Id antibody. Following immunization with the anti-La antibody the mice produced antibodies to double-stranded DNA, single-stranded DNA, Sm, SS-A/Ro, SS-B/La, and ribonucleoprotein. Furthermore, even though the anti-La antibody does not express nor react with the 16/6 Id, the immunized mice produced high titers of anti-16/6 Id antibodies as well as 16/6 Id bearing antibodies. Four months following immunization the mice exhibited significant proteinuria, and kidney sections revealed immune complex deposits on the basement membrane of the glomeruli. These results suggest that anti-La autoantibodies are involved in the induction and mediation of SLE in mice.
Fricke
H
H
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
Offen
D
D
Mendlovic
S
S
Shoenfeld
Y
Y
Bakimer
R
R
Sperling
J
J
Mozes
E
E
D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology* / Q000633:toxicity; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000633:toxicity; D001324:Autoantigens / Q000276:immunology*; D005260:Female; D007114:Immunization; D008180:Lupus Erythematosus, Systemic / Q000209:etiology*; D051379:Mice; D008809:Mice, Inbred C3H / Q000276:immunology; D012261:Ribonucleoproteins; D000097585:SS-B Antigen
D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D001324:Autoantigens; D012261:Ribonucleoproteins; D000097585:SS-B Antigen