Acid and alkali as aids in differentiation of pathogenic Nocardia and for their isolation from clinical specimens.
The tolerance of Nocardia brasiliensis, Nocardia asteroides and Nocardia caviae to different concentrations of acids and alkalis was studied in this work. The purpose was to determine their use: (a) in the differentiation of species; (b) for decontamination of clinical materials during the isolation of pathogenic Nocardia. The results showed (1) that both 2 M NH3 and 0.05 M H2SO4 solutions are useful for differentiating species. They can also be used as a complementary method for identification. (2) That both 1 M NH3 and 0.05 M H2SO4 solutions were useful for decontamination of clinical materials during the isolation of pathogenic Nocardia.
van Gelderen de Komaid
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Cátedra de Micología, Instituto de Microbiología Dr. Luis C. Verna, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Repblica Argentina.
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D000085:Acetates / Q000494:pharmacology; D019342:Acetic Acid; D000143:Acids / Q000494:pharmacology*; D000468:Alkalies / Q000494:pharmacology*; D000641:Ammonia / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006878:Hydroxides / Q000494:pharmacology; D009615:Nocardia / Q000187:drug effects / Q000302:isolation & purification*; D009617:Nocardia Infections / Q000382:microbiology*; D009616:Nocardia asteroides / Q000187:drug effects / Q000302:isolation & purification; D011188:Potassium / Q000494:pharmacology; D017680:Potassium Compounds; D012988:Soil Microbiology; D013464:Sulfuric Acids / Q000494:pharmacology
D000085:Acetates; D000143:Acids; D000468:Alkalies; D006878:Hydroxides; D017680:Potassium Compounds; D013464:Sulfuric Acids; D000641:Ammonia; C033158:sulfuric acid; D019342:Acetic Acid; D011188:Potassium; C029943:potassium hydroxide