[Hybridase cleavage of RNA. II. Automatic synthesis of mixed oligonucleotide probes].
Several oligo(ribodeoxyribo)nucleotides to be used as probes in the RNase H-induced hydrolyses of 5S ribosomal RNA E. coli were synthesized on the Victoria-4M automatic gene synthesizer by the phosphoramidite approach, which allows for introducing ribonucleotides into any position of the oligomer. 2'-Hydroxy function was protected by tert-butyldimethylsilyl group whose hydrophobicity simplified isolation of the oligonucleotides by reverse-phased HPLC.
Romanova
E A
EA
Oretskaia
T S
TS
Sukhomlinov
V V
VV
Krynetskaia
N F
NF
Metelev
V G
VG
Shabarova
Z A
ZA
D001345:Autoradiography; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000138:chemical synthesis*
D015345:Oligonucleotide Probes
Gibridaznoe rasshcheplenie RNK. II. Avtomaticheskiĭ sintez smeshannykh oligonukleotidnykh zondov.