title: Suppression of alveolar macrophage membrane-receptor-mediated phagocytosis by model particle-adsorbate complexes: physicochemical moderators of uptake.
abstract: In order to assess the abilities of alveolar macrophages (AMs) to phagocytize adsorbent-adsorbate complexes, rat AMs were incubated in vitro with two carbon blacks that have 15-fold differences in specific surface areas (ASTM classification N339 less than Black Pearls 2000) sorbed with 0.5 and 1.0 monolayer coverages of a polar and semi-polar adsorbate (acrolein and benzofuran, respectively). One-half monolayer coverages of N339 with either adsorbates significantly suppressed the phagocytosis of the carbon black, whereas one monolayer coverage did not. Neither adsorbate at either coverages affected the phagocytosis of Black Pearls 2000. The capacity of macrophages to phagocytize a subsequent particle challenge via the Fc-membrane receptor was quantified following treatment of the macrophages with the carbon black-adsorbate complexes. Treatment of the macrophages with carbon black N339-adsorbates complexes at both coverages impaired Fc-receptor-mediated phagocytosis, whereas no effect was observed when the carbon black was Black Pearls 2000. The results of this study indicate that the surface properties of the particles, the chemical properties of the chemical pollutants, and the interactions between particles and pollutants play a major role in defining the biological effect of particle-pollutant complexes.
authors.affiliation: Department of Environmental Health Sciences, Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD 21205.
authors.forename: G J
authors.initials: GJ
authors.lastname: Jakab
authors.forename: T H
authors.initials: TH
authors.lastname: Risby
authors.forename: S S
authors.initials: SS
authors.lastname: Sehnert
authors.forename: R R
authors.initials: RR
authors.lastname: Hmieleski
authors.forename: M I
authors.initials: MI
authors.lastname: Gilmour
mesh_terms: D000327:Adsorption; D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D002244:Carbon; D066298:In Vitro Techniques; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects; D011650:Pulmonary Alveoli / Q000187:drug effects* / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D011961:Receptors, Fc
chemical_list: D000393:Air Pollutants; D011961:Receptors, Fc; D002244:Carbon