title: Immunological characterization of L-triiodothyronine and L-thyroxine labelled with rhodamine B isothiocyanate.
abstract: In the present work the label of L-T3 and L-T4 with RBITC have been developed by means of a relatively easy procedure. A purification by TLC, previously optimized, offers the possibility of obtaining a good recovery of conjugates T3 and T4-RBITC. The immunoreactivity of conjugates was calculated by a formulas especially designed for this purpose. This formulas could be applied to any molecule labelled with fluorescent compounds.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Málaga, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Reyes
authors.forename: J L
authors.initials: JL
authors.lastname: Diéguez
authors.forename: M
authors.initials: M
authors.lastname: Ruiz
authors.forename: A
authors.initials: A
authors.lastname: Alonso
authors.forename: M
authors.initials: M
authors.lastname: Morell
mesh_terms: D002855:Chromatography, Thin Layer; D005456:Fluorescent Dyes; D011863:Radioimmunoassay; D012235:Rhodamines; D013050:Spectrometry, Fluorescence; D013974:Thyroxine / Q000032:analysis* / Q000276:immunology; D014284:Triiodothyronine / Q000032:analysis* / Q000276:immunology
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; D014284:Triiodothyronine; C027755:rhodamine isothiocyanate; D013974:Thyroxine