c-yes protein kinase is associated with a 38 kD protein in cerebellum.
p62c-yes, the protein product of the yes proto-oncogene, was found in association with a cellular protein of 38 kD in chicken cerebella. The complex was detected by immunoprecipitation of cerebellar membranes with affinity purified anti-yes IgG followed by in vitro phosphorylation of the immunocomplex. Both proteins were found to be phosphorylated exclusively on tyrosine. The sedimentation profile of the yes kinase indicated that a fraction of p62c-yes was complexed with the 38 kD protein and comigrated in the gradient with a molecular mass of approximately 150 kD. We have previously described the association of p60c-src with a 38 kD protein, referred to as p38 [Grandori, C. and Hanafusa, H., J. Cell Biol. (1988), 107: 2125-2135]. Comparison of the src-associated p38 with the yes-associated 38 kD protein indicates that they are indistinguishable by one-dimensional peptide mapping. Association of p38 with more than one member of the src-family of tyrosine kinases makes this protein an attractive probe to study the structural and functional aspects of these enzymes.
Grandori
C
C
Laboratory of Molecular Oncology, Rockefeller University, New York, New York 10021.
Sudol
M
M
Hanafusa
H
H
D000818:Animals; D002531:Cerebellum / Q000737:chemistry / Q000378:metabolism*; D002645:Chickens; D010766:Phosphorylation; D011233:Precipitin Tests; D011494:Protein Kinases / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000378:metabolism*; D051575:Proto-Oncogene Proteins c-yes; D019061:src-Family Kinases
D011518:Proto-Oncogene Proteins; D011494:Protein Kinases; D051575:Proto-Oncogene Proteins c-yes; D019061:src-Family Kinases