[Novel PCR methods for detection of HIV proviruses using digoxigenin labelled probes--filter paper method].
We have reported novel methods for screening of HIV by using polymerase chain reaction (PCR) and digoxigenin labelled probes. Here we developed a filter paper method for samples from long distances. About 50 microliters of peripheral bloods were absorbed in filters (5 mm diameter), dried, heated, then stored for 1 month at room temperature before PCR. This method was proved to be sufficient for detection of HIV proviruses from clinical samples.
Yamaguchi
K
K
Department of Internal Medicine, Juntendo University School of Medicine.
Ushijima
H
H
D015342:DNA Probes; D004076:Digoxigenin; D005374:Filtration; D015658:HIV Infections / Q000175:diagnosis*; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011533:Proviruses / Q000302:isolation & purification*
D015342:DNA Probes; D004076:Digoxigenin