[Molecular cloning and structural-functional analysis of the arginine biosynthesis genes of the thermophilic bacterium Bacillus stearothermophilus].
Genes encoding arginine biosynthesis of Bacillus stearothermophilus strain 718 were cloned in the mutant argA strain of the Escherichia coli K-12. The arg genes were shown to be located on the 3.7 kb DNA fragment in the following order: argA--argE--argB. The expression of the argA gene of B. stearothermophilus on the multicopy vehicle is twofold higher in argR- strain of E. coli K-12 than is isogenic argR+ strain. According to hybridization analysis argA genes of B. stearothermophilus and B. subtilis have low level of homology, which is the evidence of their evolutional divergence.
Sakanian
V A
VA
Ovespian
A S
AS
Mett
I L
IL
Kochikian
A V
AV
Petrosian
P K
PK
D001120:Arginine / Q000096:biosynthesis*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D001411:Geobacillus stearothermophilus / Q000235:genetics*; D005810:Multigene Family / Q000235:genetics; D009154:Mutation / Q000235:genetics; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping
D004269:DNA, Bacterial; D001120:Arginine
Molekuliarnoe klonirovanie i strukturno-funktsional'nyĭ analiz genov biosinteza arginina termofil'noĭ bakterii Bacillus stearothermophilus.