[Analysis of immunoprotection against fatal experimental Klebsiella pneumoniae pneumonia].
Comparatively little is known about the immunoprotective mechanisms against K. pneumoniae pneumonia. Whether the antibody against K. pneumoniae protects against K. pneumoniae pneumonia is not fully known. This study was designed to investigate some of the murine host factors responsible for immunization with K. pneumoniae DT-S to infection by aerosol inhalation with K. pneumoniae DT-S: in particular, the role of the antibody and the immunoprotective antibody which was the antibody against which component of K. pneumoniae was investigated. Mice preimmunized by aerosol inhalation with live or formalin-killed DT-S and mice preimmunized intravenously with live DT-S did not protect against infection by aerosol inhalation with DT-S. Mice preimmunized intravenously with formalin-killed DT-S were shown to protect significantly against fatal experimental K. pneumoniae pneumonia (p less than 0.01). This immunoprotection against fatal experimental pneumonia was related to titers of anti-ultrasonicates of DT-S antibody detected by an Enzyme-linked immunosorbent assay (ELISA). That this immunoprotection is dependent on the antibody against which component of DT-S was investigated by ELISA. An ELISA was performed to detected antibody against purified capsular polysaccharide (CPS), lipopolysaccharide (LPS) and outer membrane proteins (OMP). Mice preimmunized intravenously with formalin-killed DT-S were detected to have antibodies against LPS, but not to CPS and OMP. Then the immunoprotection against fatal experimental pneumonia was related to titers of anti-LPS antibody. These findings indicate that anti-LPS antibody may play a major role in the protection against fatal experimental K. pneumoniae pneumonia.
Shimoguchi
K
K
Department of Laboratory Medicine, Nagasaki University School of Medicine.
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D007114:Immunization; D007710:Klebsiella Infections / Q000276:immunology* / Q000517:prevention & control; D007711:Klebsiella pneumoniae / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011014:Pneumonia / Q000276:immunology* / Q000517:prevention & control
D000907:Antibodies, Bacterial; D008070:Lipopolysaccharides