title: Ornithine decarboxylase in Saccharomyces cerevisiae: chromosomal assignment and genetic mapping of the SPE1 gene.
abstract: The gene for ornithine decarboxylase in Saccharomyces cerevisiae, SPE1, has been assigned to chromosome XI by the technique of transverse alternating pulsed field electrophoresis and DNA-DNA hybridization. Genetic mapping by tetrad analysis shows that the SPE1 gene is located on the left arm of chromosome XI, 6 cM from the LAP1 gene and 43 cM from the TRP3 gene. The spe10 mutation previously isolated in this laboratory is mapped to the N-terminal region of the SPE1 gene, and therefore should be designated as a spe1 allele.
authors.affiliation: Laboratory of Biochemical Pharmacology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: Q W
authors.initials: QW
authors.lastname: Xie
authors.forename: C W
authors.initials: CW
authors.lastname: Tabor
authors.forename: H
authors.initials: H
authors.lastname: Tabor
mesh_terms: D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D015342:DNA Probes; D004271:DNA, Fungal / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D005816:Genetic Complementation Test; D009154:Mutation; D009693:Nucleic Acid Hybridization; D009955:Ornithine Decarboxylase / Q000235:genetics*; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*
chemical_list: D015342:DNA Probes; D004271:DNA, Fungal; D009955:Ornithine Decarboxylase