title: Involvement of calcium, calmodulin and protein phosphorylation in morphogenesis of Candida albicans.
abstract: N-Acetyl-D-glucosamine-induced germ tube formation in Candida albicans at 37 degrees C was accompanied by an increase in the rate of protein phosphorylation. The calmodulin antagonist trifluoperazine and the Ca2+ ionophore A23187, which inhibited germ tube formation, also reduced the rate of phosphorylation. The rate of phosphorylation was also reduced when cells were incubated at 25 degrees C, which favoured yeast-phase growth. Two-dimensional SDS-PAGE analysis of phosphoproteins from germ-tube-forming and yeast cells revealed two germ-tube-specific and three yeast-specific phosphoproteins. Germ tubes and hyphae had more calmodulin activity than yeast cells, irrespective of the germ-tube-inducing condition used. As a first step towards understanding the inhibitory effect of trifluoperazine on germ tube formation, calmodulin from C. albicans was purified to homogeneity. It was heat stable, and displayed a pronounced Ca2(+)-induced shift in electrophoretic mobility.
authors.affiliation: Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
authors.forename: V
authors.initials: V
authors.lastname: Paranjape
authors.forename: B G
authors.initials: BG
authors.lastname: Roy
authors.forename: A
authors.initials: A
authors.lastname: Datta
mesh_terms: D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002147:Calmodulin / Q000378:metabolism*; D002176:Candida albicans / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D009024:Morphogenesis; D010750:Phosphoproteins / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation / Q000187:drug effects; D014268:Trifluoperazine / Q000494:pharmacology*
chemical_list: D002147:Calmodulin; D005656:Fungal Proteins; D010750:Phosphoproteins; D010761:Phosphorus Radioisotopes; D014268:Trifluoperazine; D000001:Calcimycin; D002118:Calcium