Influence of chronic chlorimipramine treatment on the serotonergic modulation of acetylcholine release from guinea pig caudate nucleus slices.
The influence of chronic chlorimipramine (10 mg/kg daily s.c. for 14 days) treatment (CCT) on 5-HT modulation of basal 3H-choline overflow from guinea pig caudate nucleus slices was studied. The increase in basal tritium overflow induced by 5-HT (3-100 mumol/l) or by DOI (30-100 mumol/l) in normal slices was significantly lower in CCT slices. When tetrodotoxin 0.5 mumol/l was added, the facilitatory effect by 5-HT was cancelled, its late inhibitory effect became more evident and to the same extent in normal and CCT slices. This inhibition was cancelled by ICS 205-930 30 mumol/l. These results indicate that the 5-HT2-mediated facilitatory response of intrastriatal cholinergic neurones to 5-HT is reduced by CCT, while the inhibitory response is unaffected.
Siniscalchi
A
A
Institute of Pharmacology, University of Ferrara, Italy.
Beani
L
L
Bianchi
C
C
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D002421:Caudate Nucleus / Q000187:drug effects / Q000378:metabolism*; D002794:Choline / Q000378:metabolism; D002997:Clomipramine / Q000008:administration & dosage / Q000494:pharmacology*; D004334:Drug Administration Schedule; D005260:Female; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D011985:Receptors, Serotonin / Q000187:drug effects / Q000502:physiology; D012016:Reference Values; D012701:Serotonin / Q000494:pharmacology*; D013779:Tetrodotoxin / Q000494:pharmacology
D011985:Receptors, Serotonin; D012701:Serotonin; D013779:Tetrodotoxin; D002794:Choline; D000109:Acetylcholine; D002997:Clomipramine