[Recombinant monoclonal antibodies to the Lassa virus: the formation of reactive paratopes].
Eighteen hybrid lines secreting recombinant monoclonal antibodies to Lassa virus were produced by fusion of mouse splenocytes with antibody-secreting X-63 myeloma cells. Interrelations between the structure and reactivity of the antibodies were studied by different serological and immunochemical methods. Monoclonal antibodies were divided into different groups according to their serological properties and macromolecular structure. A comparative analysis of the structure and reactivity of the recombinant monoclonal antibodies showed that the light and heavy Ig-specific chains could form the reactive antibodies when the chains were present in different paratopes of Ig molecules.
Vladyko
A S
AS
Kunitskaia
L Ia
LIa
Kras'ko
A G
AG
Bystrova
S I
SI
Pyzhova
N S
NS
Lukashevich
I S
IS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D000915:Antibody Affinity / Q000276:immunology; D000937:Antigen-Antibody Reactions / Q000276:immunology; D001666:Binding Sites, Antibody / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D007132:Immunoglobulin Isotypes / Q000032:analysis / Q000276:immunology; D007836:Lassa virus / Q000276:immunology*; D051379:Mice; D011994:Recombinant Proteins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007132:Immunoglobulin Isotypes; D011994:Recombinant Proteins
Rekombinantnye monoklonal'nye antitela k virusu Lassa: formirovanie reaktivnykh paratopov.