[Post mortem HLA phenotyping of donors of eyes. Evaluation of a serological method of microlymphotoxicity on lymphocytes and PHA lymphoblasts].
To increase the number of HLA typed corneas a microlymphocytotoxicity assay on lymphocytes and PHA lymphoblasts was investigated. A double immunofluorescence technique using magnetic beads coated with anti-T8 (for class I) and anti-DR (for class II) monomorphic antibodies was applied. Blood samples from 50 non selected donors were obtained. HLA class I and class II typing was possible in 74% of the cases. Using PBL's (Peripheral Blood Lymphocytes) HLA class I could be defined in 29 out of 50 Cases and class II in 15 out of 50 cases. On PHA blasts HLA class I and class II antigens could be defined in 32 and 33 out of 50 cases respectively. Mean time of culture was 10 days (6-20). No influence of donor age and post partum time could be observed. By combination of both methods a significant proportion of eye donors could be reliably typed within a short time.
Delbosc
B
B
Clinique ophtalmologique, Hospices civils, Strasbourg.
Urlacher
A
A
Tongio
M M
MM
Flament
J
J
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001757:Blastomeres / Q000276:immunology; D005069:Evaluation Studies as Topic; D005123:Eye / Q000276:immunology / Q000637:transplantation*; D005455:Fluorescent Antibody Technique; D006680:HLA Antigens / Q000032:analysis / Q000235:genetics*; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D008214:Lymphocytes / Q000276:immunology; D008875:Middle Aged; D014019:Tissue Donors
D006680:HLA Antigens
Phénotypage HLA post mortem des donneurs d'yeux. Evaluation d'une méthode sérologique de microlymphocytotoxicité sur lymphocytes et lymphoblastes PHA.