Establishment of cell-to-cell contact by adoptively transferred adherent lymphokine-activated killer cells with metastatic murine melanoma cells.
A murine model of pulmonary B16 melanoma was used to study the infiltration into metastases of lymphokine-activated killer (LAK) cells and adherent lymphokine-activated killer (A-LAK) cells and, specifically, to study whether A-LAK cells are able to leave the tumor microcirculation and establish cell-to-cell contact with malignant cells. Fluorescence microscopy demonstrated that A-LAK cells accumulated in metastases twice as efficiently as LAK cells during interleukin-2 stimulation. Electron microscopy of pulmonary metastases 16 hours after administration of 2.5 x 10(7) A-LAK cells revealed A-LAK cells, identified by the presence of typical two-compartment granules, in direct contact with melanoma cells. This finding was confirmed by using A-LAK cells prelabeled with polycationized ferritin. In conclusion, our observations demonstrate unambiguously the ability of adoptively transferred A-LAK cells to establish contact with extravascular metastatic melanoma cells.
Basse
P H
PH
Department of Pathology, University of Pittsburgh School of Medicine, Pa.
Nannmark
U
U
Johansson
B R
BR
Herberman
R B
RB
Goldfarb
R H
RH
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002450:Cell Communication / Q000502:physiology*; D005293:Ferritins; D016219:Immunotherapy, Adoptive; D015979:Killer Cells, Lymphokine-Activated / Q000166:cytology* / Q000648:ultrastructure; D008113:Liver Neoplasms / Q000473:pathology / Q000556:secondary / Q000628:therapy; D008175:Lung Neoplasms / Q000473:pathology / Q000556:secondary / Q000628:therapy; D008297:Male; D008546:Melanoma, Experimental / Q000473:pathology* / Q000556:secondary / Q000628:therapy; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D012235:Rhodamines
D012235:Rhodamines; C010297:polycationic ferritin; D005293:Ferritins