Analysis of sulphonamides using supercritical fluid chromatography and supercritical fluid chromatography-mass spectrometry.
Packed-column supercritical fluid chromatography has been used for the separation of mixtures of sulphonamides on silica and amino-bonded stationary phases utilizing carbon dioxide with methanol modifier as the mobile phase. The effect of modifier concentration, column pressure and modifier identity on retention was also studied. Packed-column supercritical fluid chromatography-mass spectrometry (SFC-MS) of these mixtures utilizing both moving-belt and modified thermospray interfaces was also studied. The identification of sulphamethazine in a spiked porcine kidney extract was performed by SFC-MS using the moving-belt interface.
Perkins
J R
JR
Department of Chemistry, University College of Swansea, U.K.
Games
D E
DE
Startin
J R
JR
Gilbert
J
J
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D003080:Cold Temperature; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007668:Kidney / Q000737:chemistry; D013449:Sulfonamides / Q000032:analysis*; D013552:Swine
D013449:Sulfonamides