A possible specific receptor for 3-beta-androstanediol in the human sebaceous gland.
Dihydrotestosterone (DHT) does not seem to be the active specific metabolite of testosterone in hypertrophic sebaceous glands of subjects affected by male pattern baldness (MPB) and several results indicate that probably 3-beta-androstanediol (beta DIOL) could be an active form of testosterone in those glands. Cytosol and serum from several patients affected by MPB and subjected to hair autotransplantation, was incubated with both beta DIOL and 3-alpha-androstanediol (alpha DIOL). Binding patterns indicate that alpha DIOL binds to cytosolic proteins probably due to the contaminating sex hormone binding globulin (SHBG), whereas beta DIOL exhibits an atypical binding process in cytosol in the presence of high concentrations of non radioactive beta DIOL. This binding increases progressively up to 2 pmol/mg protein at the limit solubility conditions for the non radioactive steroid. This pattern is not observed in serum from the same patients, where the binding of beta DIOL is typically restricted to the SHBG. These results strongly suggest the existence of a specific beta DIOL-binding protein in the hypertrophic sebaceous glands and explain the lack of specific receptor for DHT in these tissues.
Caballero
M J
MJ
Unidad de Farmacología, Facultad de Medicina, Universidad de Barcelona, Reus/Tarragona, Spain.
Carreras
E
E
Mallol
J
J
D000505:Alopecia / Q000378:metabolism / Q000601:surgery; D001378:Azasteroids / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D013196:Dihydrotestosterone / Q000031:analogs & derivatives* / Q000378:metabolism; D006197:Hair / Q000637:transplantation; D006801:Humans; D006984:Hypertrophy; D008297:Male; D011944:Receptors, Androgen / Q000378:metabolism*; D012627:Sebaceous Glands / Q000378:metabolism* / Q000473:pathology; D012738:Sex Hormone-Binding Globulin / Q000378:metabolism; D014182:Transplantation, Autologous
D001378:Azasteroids; D011944:Receptors, Androgen; D012738:Sex Hormone-Binding Globulin; C038884:androstane-3,17-diol receptor; D013196:Dihydrotestosterone; C031563:17-N,N-diethylcarbamoyl-4-methyl-4-azaandrostane-3-one