Preparation of diethylaminoethyl hollow fibres for high-flow liquid chromatography.
A novel anion-exchange column for fast-flow liquid chromatography was developed. The column was prepared by immobilizing diethylaminoethyl groups on to the inner walls of regenerated cellulose hollow fibres. A detailed investigation was conducted of the parameters affecting diethylaminoethyl immobilization such as the NaOH concentration, the reaction temperature, the diethylaminoethyl concentration and the reaction span. Based upon the findings, a protocol to optimize the preparation of the diethylaminoethyl hollow fibres was established. The diethylaminoethyl hollow fibres prepared adsorbed 186 +/- 30 mg of albumin/g fibres.
Tsuei
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College of Pharmacy, University of Michigan, Ann Arbor 48109-1065.
Yang
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D000327:Adsorption; D002852:Chromatography, Ion Exchange / Q000295:instrumentation*; D004048:Diethylamines / Q000737:chemistry*; D008970:Molecular Weight; D012972:Sodium Hydroxide / Q000494:pharmacology; D013696:Temperature
D004048:Diethylamines; D012972:Sodium Hydroxide