title: Suppression of water shift into intracellular space by TA-3090 (calcium entry blocker) measured with NMR.
abstract: In order to clarify the effects of TA-3090 (calcium entry blocker) on the suppression of water after ischaemic events, 16 measurements of T2f were continuously performed on brain biopsy (for 2-60 min) obtained from treated and control Wistar rats. The time constant (k) and NMR parameters (T2f,(O) delta T2f, T2fmax(T2f(O) + delta T2f) were obtained from 16 values of T2f. The values of k in Wistar rats treated with intravenously administrated TA-3090 (0.5 mg/kg) were significantly prolonged as compared to that of control. There were no significant differences of maximum prolongation of T2f(T2fmax(T2f(O) + delta T2f) among three groups. Since the prolongation of T2f after biopsy reflects the water shift from extra to intracellular space, the increments of time constant indicates that TA-3090 suppresses the water shift into intracellular space. Our present results suggest that TA-3090 prevents some processes involved in irreversible cell damage and suppresses the cytotoxic brain oedema in the incomplete ischaemic area where non-competitive calcium channel is inactive.
authors.affiliation: Department of Neurology, Tokai University School of Medicine, Kanagawa, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.forename: M
authors.initials: M
authors.lastname: Haida
authors.forename: R
authors.initials: R
authors.lastname: Taniguchi
authors.forename: K
authors.initials: K
authors.lastname: Yazaki
authors.forename: D
authors.initials: D
authors.lastname: Kurita
authors.forename: M
authors.initials: M
authors.lastname: Fukuzaki
authors.forename: Y
authors.initials: Y
authors.lastname: Shinohara
mesh_terms: D000818:Animals; D001834:Body Water / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D004110:Diltiazem / Q000031:analogs & derivatives* / Q000494:pharmacology; D007424:Intracellular Fluid / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D002121:Calcium Channel Blockers; C056595:clentiazem; D004110:Diltiazem