title: [The dependence of the formation of stromal CFU-f colonies on the stimulating action of hematopoietic cells].
abstract: CFU-f-derived stromal colony formation was accomplished in adherent marrow cell cultures (AMCC) with serum-rich medium. It turned out to require additional stimulation by hemopoietic feeder cells: by irradiated marrow cells and spleen cells if they possess megakaryocytes and platelets or by platelets from the blood. PDGF, EGF and IL-3 did not substitute the colony stimulating activity of feeder cells. Thymus, lymph node cells and blood leucocytes had no colony stimulating activity. At low oxygen concentrations which improve colony formation the stimulating activity of hemopoietic feeder cells was expressed, as well. Thus, CFU-f colony formation depends on stimulation by hemopoietic cells in addition to serum growth factors. In full populations of marrow cells the CFU-f colony formation is stimulated by marrow cells which accompany the CFU-f.
authors.forename: N V
authors.initials: NV
authors.lastname: Latsinik
authors.forename: A Ia
authors.initials: AIa
authors.lastname: Fridenshteĭn
authors.forename: Iu F
authors.initials: IuF
authors.lastname: Gorskaia
authors.forename: I L
authors.initials: IL
authors.lastname: Moskvina
authors.forename: Kh Ch
authors.initials: KhCh
authors.lastname: Iuĭ
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000528:radiation effects; D001854:Bone Marrow Cells; D002448:Cell Adhesion / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D003114:Colony-Forming Units Assay / Q000379:methods; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D006168:Guinea Pigs; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D007377:Interleukin-3 / Q000494:pharmacology; D051379:Mice; D008808:Mice, Inbred CBA; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology
chemical_list: D007377:Interleukin-3; D010982:Platelet-Derived Growth Factor; D004815:Epidermal Growth Factor
vernacular_title: Zavisimost' obrazovaniia stromal'nykh KOKf-koloniĭ ot stimuliruiushchego vozdeĭstviia gemopoéticheskikh kletok.