Isolation of intestinal epithelial cells and evaluation of transport functions.
Epithelial cells can be isolated from the small intestine of chickens by a procedure involving hyaluronidase treatment of the intact tissue. The isolated cells retain a high degree of functional activity as assessed by the formation of 70-fold gradients of alpha-MG. Stability of the sugar gradients reflects maintenance of stable electrochemical Na+ gradients across the plasma membrane. The cells can be used to evaluate the properties of Na(+)-dependent sugar transport, Na(+)-independent sugar transport, ion transport, metabolism, membrane potentials, and the integration of these events, all of which are important to achieving a stable sugar gradient.
Kimmich
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GA
D000818:Animals; D001692:Biological Transport; D002250:Carbon Radioisotopes; D002469:Cell Separation / Q000379:methods; D002645:Chickens; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology; D007202:Indicators and Reagents; D007413:Intestinal Mucosa / Q000166:cytology* / Q000502:physiology; D007700:Kinetics; D008564:Membrane Potentials; D008757:Methylglucosides / Q000378:metabolism; D009861:Onium Compounds / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D011865:Radioisotope Dilution Technique
D002250:Carbon Radioisotopes; D007202:Indicators and Reagents; D008757:Methylglucosides; D009861:Onium Compounds; D009943:Organophosphorus Compounds; C027020:methylglucoside; C013289:tetraphenylphosphonium