Cloning and nucleotide sequence of a chromosomally encoded tetracycline resistance determinant, tetA(M), from a pathogenic, methicillin-resistant strain of Staphylococcus aureus.
This report describes the cloning and sequencing of a chromosomally encoded tetracycline resistance determinant from a clinical isolate of methicillin-resistant Staphylococcus aureus. On the basis of the sequence, the gene is in the tet(M) class, and it was shown that the S. aureus tetA(M) gene is induced at the level of transcription.
Nesin
M
M
Department of Biochemistry, New York University Medical Center, New York 10003.
Svec
P
P
Lupski
J R
JR
Godson
G N
GN
Kreiswirth
B
B
Kornblum
J
J
Projan
S J
SJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D016106:Methicillin Resistance / Q000235:genetics*; D008969:Molecular Sequence Data; D013211:Staphylococcus aureus / Q000235:genetics* / Q000648:ultrastructure; D013753:Tetracycline Resistance / Q000235:genetics*; D014158:Transcription, Genetic