[The detection of viral antigens and ribosomal RNA in cells by using low-temperature media].
A post-embedding technique for immunocytochemical analysis at the ultrastructural level was used to detect and localize HIV antigens on ultrathin sections of Lowicryl-embedded HIV-infected cells. A genomic probe containing ribosomal sequences and labeled with biotin was used to hybridize rRNA molecules in sections of animal cells embedded in Lowicryl. The method presently described offers the possibility to detect rapidly and precisely ribosomal gene expression and viral proteins at the ultrastructural level.
Grigor'ev
V B
VB
Kadoshnikov
Iu P
IuP
Escaig
F
F
Fournier
J G
JG
Rudneva
I A
IA
Karamov
E V
EV
Kazennova
E V
EV
Garaev
M M
MM
D000180:Acrylic Resins; D000818:Animals; D015342:DNA Probes; D005786:Gene Expression Regulation / Q000235:genetics; D015488:HIV Antigens / Q000032:analysis*; D015497:HIV-1 / Q000276:immunology*; D015498:HIV-2 / Q000276:immunology*; D006801:Humans; D008722:Methods; D009693:Nucleic Acid Hybridization; D012335:RNA, Ribosomal / Q000032:analysis* / Q000235:genetics; D013696:Temperature
D000180:Acrylic Resins; D015342:DNA Probes; D015488:HIV Antigens; D012335:RNA, Ribosomal; C037883:Lowicryl 4KM resin
Vyiavlenie virusnykh antigenov i ribosomal'noĭ RNK v kletkakh s ispol'zovaniem nizkotemperaturnykh sred.