[The evaluation of heterosexual transmission of the human immunodeficiency virus by nucleic acid amplification technics].
Heterosexual couples of individuals infected by HIV represent a group of high risk of virus infection. We have investigated the presence of fragments of the HIV genome with a new nucleic acid amplification technique (PCR or polymerase chain reaction) in lymphocytes from 33 seronegative couples with anti-HIV antibodies, most of which were heroin addicts. 12 of the 33 couples (36%) had positive PCR for a gag HIV amplimer in the absence of antibodies. 4 of them (12%) also showed reactivity for sequences from an env amplimer. A control group of 7 patients with AIDS and 4 seropositive couples showed reactivity to gag and env in all. It is concluded that silent HIV infection may frequently develop in heterosexual couples of infected individuals.
Soriano
V
V
Unidad de Enfermedades Infecciosas, Hospital de Badalona Germans Trias i Pujol, Universidad Autónoma de Barcelona.
Hewlett
I
I
Tor
J
J
de Amo
C
C
Lázaro
I
I
Muga
R
R
Clotet
R
R
Ribas-Mundó
M
M
Foz
M
M
D015492:AIDS Serodiagnosis; D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis / Q000235:genetics / Q000635:transmission*; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D005784:Gene Amplification / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics*; D006679:HIV Seropositivity / Q000175:diagnosis / Q000235:genetics / Q000635:transmission; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008297:Male; D021141:Nucleic Acid Amplification Techniques; D012725:Sexual Behavior
D004279:DNA, Viral
Evaluación de la transmisión heterosexual del virus de la inmunodeficiencia humana por técnicas de amplificación de ácidos nucleicos.