title: Reactivity of Limulus amoebocyte lysate towards (1----3)-beta-D-glucans.
abstract: The structure activity relationship for beta-D-glucans for the gelation of the amoebocyte lysates of the horseshoe crab (Limulus) has been investigated. beta-D-Glucans that had no (1----3) linkages induced little or no gelation. The (1----3)-beta-D-glucans curdlan (unbranched), grifolan (approximately 33% branched), schizophyllan (approximately 33% branched), lentinan (approximately 40% branched). SSG (approximately 50% branched), and OL-2 (approximately 66% branched) induced significant gelation. The optimum concentration for gelation was correlated with the content of branching. Single chain (rather than a triple helix) conformation and higher molecular weight were associated with higher reactivity.
authors.affiliation: Laboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Ohno
authors.forename: Y
authors.initials: Y
authors.lastname: Emori
authors.forename: T
authors.initials: T
authors.lastname: Yadomae
authors.forename: K
authors.initials: K
authors.lastname: Saito
authors.forename: A
authors.initials: A
authors.lastname: Masuda
authors.forename: S
authors.initials: S
authors.lastname: Oikawa
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D005936:Glucans / Q000737:chemistry / Q000378:metabolism*; D008033:Limulus Test; D008970:Molecular Weight; D013329:Structure-Activity Relationship; D047071:beta-Glucans
chemical_list: D005936:Glucans; D047071:beta-Glucans; C033363:beta-1,3-glucan