Use of two-dimensional thin-layer chromatography for the components study of poly(adenosine diphosphate ribose).
Two-dimensional thin-layer chromatography on cellulose plates has been used for separating and quantifying the three adenosine derivatives: AMP, phosphoribosyl AMP (PRAMP), and (PR)2AMP obtained by venom phosphodiesterase digestion of poly(ADP-ribose). In vitro synthesized polymer, up to 300 derivatives in length were studied. Some parameters of the complexity of poly(ADP-ribose) could be deduced from our results: (i) The first branching point appears in fragments of approximately 21 derivatives in length. (ii) The branching points are located at regular distances of approximately 41 derivatives from each other.
Keith
G
G
IBMC du CNRS et de l'Université Louis Pasteur, Strasbourg, France.
Desgrès
J
J
de Murcia
G
G
D000249:Adenosine Monophosphate / Q000031:analogs & derivatives* / Q000032:analysis* / Q000302:isolation & purification; D002855:Chromatography, Thin Layer / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D011064:Poly Adenosine Diphosphate Ribose / Q000032:analysis* / Q000302:isolation & purification; D011108:Polymers
D011108:Polymers; C016664:2'-(5''-phosphoribosyl)-5'-adenosine monophosphate; D011064:Poly Adenosine Diphosphate Ribose; D000249:Adenosine Monophosphate; C068259:diphosphoribosyl-adenosine monophosphate