[Immunomodulating effect of lysed immunoactive fractions of selected Escherichia coli strains on the macrophage system. An in vitro study].
The immunomodulator Uro-Vaxom (an immunoactive fraction of E. coli, FEC) is a therapeutic agent used to control bacterial infections. FEC was applied to macrophages of C57BL/6 mice to investigate the in vitro activation of these cells of the unspecific immune system. Experiments were designed to test the secretory, immuno-regulatory and cytotoxic function of macrophages after application of FEC. As presented here, production of Interleukin-6 and tumor-necrosis-factor were significantly and dose-dependent way increased, whereas it was not possible to induce a secretion of Interleukin-1. In addition, FEC activated macrophages kill Leishmania donovani promastigotes, Candida albicans and Staphylococcus aureus. In comparison to a pressed echinaceae-preparation, FEC activated mouse macrophages secrete Interleukin-6 and tumor-necrosis-factor and kill protozoa, fungi and bacteria, with higher efficiency.
Hockertz
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Abteilung Immunbiologie, Immuntoxikologie Fraunhofer-Institut für Toxikologie und Aerosolforschung, Hannover.
D000818:Animals; D002176:Candida albicans / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D004926:Escherichia coli / Q000276:immunology*; D005260:Female; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology; D007893:Leishmania donovani / Q000276:immunology; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010529:Peritoneal Cavity / Q000166:cytology; D013211:Staphylococcus aureus / Q000276:immunology; D014407:Tumor Cells, Cultured / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
D007375:Interleukin-1; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha
Immunmodulierende Wirkung von lysierten, immunaktiven Fraktionen aus ausgewählten Escherichia-coli-Stämmen auf das Makrophagensystem. Eine In-vitro-Studie.