title: FIA-spectrophotometric assay of N-acetylcysteine by o-phthalaldehyde derivatization.
abstract: A flow injection-spectrophotometric procedure is proposed for the determination of N-acetylcysteine (NAC). The procedure is based on the rapid reaction of the thiol group with o-phthalaldehyde and isoleucine at pH 9.5 to give an 1-alkylthio-2-alkyl-substituted isoindole which shows maximum absorbance at 335 nm. The linear dynamic range is 16-160 micrograms/ml, with a 0.6% relative standard deviation for 100 micrograms/ml. The sampling rate is 126 samples/h. The method is applied successfully to the evaluation of NAC in commercial formulations.
authors.affiliation: Departamento de Química Analítica, Facultad de Química, Universidad de Valencia, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Medina Hernández
authors.forename: M C
authors.initials: MC
authors.lastname: García Alvarez-Coque
authors.forename: E
authors.initials: E
authors.lastname: Bonet Domingo
authors.forename: R M
authors.initials: RM
authors.lastname: Villanueva Camañas
mesh_terms: D000111:Acetylcysteine / Q000032:analysis*; D007202:Indicators and Reagents; D011208:Powders; D012996:Solutions; D013053:Spectrophotometry; D009764:o-Phthalaldehyde / Q000737:chemistry
chemical_list: D007202:Indicators and Reagents; D011208:Powders; D012996:Solutions; D009764:o-Phthalaldehyde; D000111:Acetylcysteine