Isolation of Catharanthus alkaloids by solid-phase extraction and semipreparative HPLC.
A combination of moderate-pressure chromatography on C18 sorbent and preparative HPLC is developed for rapid isolation of alkaloids from Catharanthus roseus. The procedure is optimized for vindoline and catharanthine with respective yields of 3 and 2 mg per 1 g of dried leaves of the plant. The methodology is also applied for identification of the above and other alkaloids from cultured plant cells.
Naaranlahti
T
T
Department of Pharmaceutical Chemistry, University of Kuopio, Finland.
Nordström
M
M
Lapinjoki
S P
SP
Huhtikangas
A
A
Lounasmaa
M
M
D000470:Alkaloids / Q000302:isolation & purification*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D010946:Plants, Medicinal / Q000032:analysis* / Q000166:cytology; D014747:Vinblastine / Q000031:analogs & derivatives / Q000302:isolation & purification; D014748:Vinca Alkaloids / Q000302:isolation & purification
D000470:Alkaloids; D014748:Vinca Alkaloids; C009667:vindoline; D014747:Vinblastine; C017836:catharanthine