title: The influence of CS-3000 plateletapheresis on blood donors and on platelet concentrates.
abstract: Platelet concentrates were prepared from blood of donors by means of Fenwal CS-3000, or by double centrifugation method. Platelets from CS-3000 were cryopreserved with 5% DMSO for 2-4 weeks. After thawing and washing platelets were stored in room temperature for 6 hours. Immediately after plateletapheresis the platelet count decreased by 24% and leukocyte count, Ht and Hb diminished on average 30%, 22% and 18% respectively. The efficiency of separation procedure was 45% and white cells contamination 5.8 +/-8.1 x 10(11). Significant morphological changes and the decrease in total ATP comparing to double centrifugation method were observed. Storage of platelets for 6 hours in room temperature didn't cause their quality changes. Single donor plateletapheresis method can prevent alloimmunization and diminish the risk of transmission of the viruses.
authors.affiliation: Instytut Hematologii, Warszawa, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Kotelba-Witkowska
authors.forename: Z R
authors.initials: ZR
authors.lastname: Mrowiec
authors.forename: J
authors.initials: J
authors.lastname: Daszyński
authors.forename: E
authors.initials: E
authors.lastname: Skrzeczkowska
authors.forename: B
authors.initials: B
authors.lastname: Stachecki
authors.forename: M
authors.initials: M
authors.lastname: Kaszkowiak
authors.forename: E
authors.initials: E
authors.lastname: Jakuboszczak
mesh_terms: D001782:Blood Donors; D001792:Blood Platelets / Q000502:physiology*; D015925:Cryopreservation; D004121:Dimethyl Sulfoxide; D006801:Humans; D010976:Platelet Count; D010983:Plateletpheresis / Q000379:methods*
chemical_list: D004121:Dimethyl Sulfoxide