Assay for the soluble interleukin-2 receptor by sandwich enzyme linked immunosorbent assay.
A system for the detection of soluble IL-2R by sandwich enzyme linked immunosorbent assay (ELISA) was established. This assay system has good reproducibility, and was found to be specific for soluble IL-2R by examination of the binding of anti-IgM antibody or anti-IL-2R monoclonal antibody (mAb) with IgM or soluble IL-2R and inhibition by IL-2 of the binding of anti-IL-2R mAb to soluble IL-2R. The IL-2R molecules in supernatant of phytohemagglutinin-stimulated peripheral blood mononuclear cells were of 44,000-61,000 molecular weight as estimated by size of exclusion high performance liquid chromatography. The results of determinations for soluble IL-2R with a Eurogenetics kit (sandwich enzyme linked immunosorbent assay) were significantly correlated with those by the our method.
Takaishi
M
M
Department of Clinical Pathology, Hiroshima University School of Medicine.
Akiyama
M
M
Hayashi
T
T
Hirai
Y
Y
Murakami
Y
Y
Ueda
R
R
Tatsugawa
K
K
Yamakido
M
M
Tsubokura
T
T
D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis; D007376:Interleukin-2 / Q000494:pharmacology; D008970:Molecular Weight; D015375:Receptors, Interleukin-2 / Q000032:analysis* / Q000276:immunology; D012995:Solubility
D007075:Immunoglobulin M; D007376:Interleukin-2; D015375:Receptors, Interleukin-2