title: Assessment of biological reagents for presence of human immunodeficiency virus-1 (HIV-1) antigens.
abstract: We tested 122 biological reagents including laboratory quality assurance sera and therapeutic human immune serum globulin products using an immunoenzymometric assay (IEMA) for HIV-1 antigens. Biological reagents tested were 64 HIV-1 antibody non-reactive and 44 HIV-1 antibody reactive quality assurance samples, and 14 HIV-1 antibody reactive human immune serum globulins from 21 manufacturers. Twenty-one of these biological reagents were previously reported by us as Western blot reactive. All 122 samples tested were non-reactive for HIV-1 antigens. Low incidence of HIV-1 antigen in these biological reagents should not alter laboratory safety practices in which all samples are considered infectious. Use of HIV-1 antigen measurements, either alone or with HIV-1 antibody determinations does not increase the likelihood of detecting HIV-1 reactive samples.
authors.affiliation: Department of Pathology and Laboratory Medicine, UCLA School of Medicine.
authors.forename: P J
authors.initials: PJ
authors.lastname: Howanitz
authors.forename: J H
authors.initials: JH
authors.lastname: McBride
authors.forename: D O
authors.initials: DO
authors.lastname: Rodgerson
mesh_terms: D015492:AIDS Serodiagnosis; D015483:HIV Antibodies / Q000032:analysis* / Q000276:immunology; D015488:HIV Antigens / Q000032:analysis*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000592:standards*; D011786:Quality Control
chemical_list: D015483:HIV Antibodies; D015488:HIV Antigens; D007136:Immunoglobulins