title: Direct radioimmunoassay for diethylstilbestrol in serum.
abstract: A radioimmunoassay method for the measurement of diethylstilbestrol directly on 50 microliters of human serum was established using a tritium-labeled radioligand and a double antibody as a separation reagent. The assay was sensitive (less than 0.17 micrograms/L), reproducible (intra-assay and interassay coefficient of variation values, 8.14% and 8.25%, respectively), and accurate (recovery up to 95%). Factors influencing the assay are identified and discussed.
authors.affiliation: National Center for Scientific Research Demokritos, Athens, Greece.
authors.forename: E V
authors.initials: EV
authors.lastname: Economou
authors.forename: S E
authors.initials: SE
authors.lastname: Kakabakos
authors.forename: G P
authors.initials: GP
authors.lastname: Evangelatos
authors.forename: D S
authors.initials: DS
authors.lastname: Ithakissios
mesh_terms: D001798:Blood Proteins / Q000494:pharmacology; D004054:Diethylstilbestrol / Q000097:blood* / Q000627:therapeutic use; D000431:Ethanol / Q000494:pharmacology; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000097:blood / Q000188:drug therapy; D011863:Radioimmunoassay / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D012709:Serum Albumin / Q000494:pharmacology
chemical_list: D001798:Blood Proteins; D012709:Serum Albumin; D000431:Ethanol; D004054:Diethylstilbestrol