[The assessment of factor VIII-related antigen in endothelial cells of pulmonary blood vessels in multiple organ failure].
Twenty-five rabbits were randomized into test group (n = 20) and control group (n = 5). Test group animals were fed E coli [(0.4-0.6) x 10(11)/kg] by gastro-lavage and followed by hemorrhagic shock (MBP 40 mmHg for an hour) plus rapid reinfusion with the shed blood. The control group served as operation control. Twelve animals in test group developed MOF (60%). The application of the PAP method in assaying the amount of F VIIIR: Ag in endothelial cells of pulmonary blood vessels. The result showed that the scores of test group was 0.66 +/- 0.24 and the control group 3.01 +/- 0.22. The difference between two groups was statistically significant (P less than 0.05). The PaO2 in MOF animals was 63.1 +/- 9.6 mmHg and the control group 93.6 +/- 4.6 mmHg. The decrease of PaO2 and value scores of FVIIIR: Ag was closely related (r = 0.72, P less than 0.01). The result suggests that a large amount of F VIIIR: Ag was released into blood due to the endothelial injury of pulmonary blood vessels in MOF. The injury of endothelial cells in pulmonary blood vessels was an important factor in pathogenesis of MOF.
Wei
S J
SJ
Department of pathology, Medical College of GLD, Beijing, China.
D000818:Animals; D002196:Capillaries; D004730:Endothelium, Vascular / Q000276:immunology*; D008168:Lung / Q000098:blood supply*; D008297:Male; D009102:Multiple Organ Failure / Q000276:immunology*; D011817:Rabbits; D014841:von Willebrand Factor / Q000032:analysis*
D014841:von Willebrand Factor