New distinctions between regions of centromeric heterochromatin in human chromosomes by treatments with the isoschizomers NdeII-Sau3AI.
The isoschizomers NdeII-Sau3AI (decreases GATC) have been used to characterize heterochromatic regions in human chromosomes. The findings with NdeII are identical with those previously published with MboI, but the results with Sau3AI provide evidence for new distinctions of centromeric heterochromatin in chromosomes 5 and 6. The results are discussed in relation to the chromatin organization at these regions an the mechanisms of the action of restriction endonucleases.
Ludenã
P
P
Departamento de Biologia, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
Sentís
C
C
Fernández-Peralta
A M
AM
Gonzalez-Aguilera
J
J
Fernández-Piqueras
J
J
D002503:Centromere / Q000648:ultrastructure*; D002871:Chromosome Banding; D002877:Chromosomes, Human / Q000648:ultrastructure; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D005260:Female; D006570:Heterochromatin / Q000378:metabolism*; D006801:Humans; D007621:Karyotyping; D008297:Male
D006570:Heterochromatin; D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases