Experiments on a new hollow fiber membrane for immuno isolated transplantation of islets of Langerhans.
Immunoisolated transplantation of xenogeneic islets could solve problems concerning the immunology of islet transplantation. This study presents results from in vitro and in vivo experiments in rodents by the use of a PEEK-hollow fiber. Glucagon secretion of encapsulated islets during a 48-hour-culture period sustained on the same level from day 2 to 28. There were no significant differences in glucose (16.7 mmol/l)-stimulated insulin release after 6, 14 or 28 days in culture. Contrary, intraperitoneal transplantation of 800 encapsulated islets resulted in a normoglycemia of 3.8 days (medium survival time) which was similar to that of not encapsulated controls. It was concluded that a more open ultrastructure of the membrane tested could result in a minimization of the diffusion distance and overcome principle geometric problems of the hollow fiber model.
Zekorn
T
T
Medizinische Klinik III, Justus Liebig Universität, Giessen, Germany.
Renardy
M
M
Planck
H
H
Zschocke
P
P
Bretzel
R G
RG
Siebers
U
U
Federlin
K
K
D000818:Animals; D001187:Artificial Organs; D003921:Diabetes Mellitus, Experimental / Q000601:surgery*; D005934:Glucagon / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D016381:Islets of Langerhans Transplantation / Q000276:immunology / Q000379:methods*; D008297:Male; D008567:Membranes, Artificial; D051381:Rats; D011917:Rats, Inbred Lew
D007328:Insulin; D008567:Membranes, Artificial; D005934:Glucagon