title: Isolation of intestinal epithelial cells and evaluation of transport functions.
abstract: Epithelial cells can be isolated from the small intestine of chickens by a procedure involving hyaluronidase treatment of the intact tissue. The isolated cells retain a high degree of functional activity as assessed by the formation of 70-fold gradients of alpha-MG. Stability of the sugar gradients reflects maintenance of stable electrochemical Na+ gradients across the plasma membrane. The cells can be used to evaluate the properties of Na(+)-dependent sugar transport, Na(+)-independent sugar transport, ion transport, metabolism, membrane potentials, and the integration of these events, all of which are important to achieving a stable sugar gradient.
authors.forename: G A
authors.initials: GA
authors.lastname: Kimmich
mesh_terms: D000818:Animals; D001692:Biological Transport; D002250:Carbon Radioisotopes; D002469:Cell Separation / Q000379:methods; D002645:Chickens; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology; D007202:Indicators and Reagents; D007413:Intestinal Mucosa / Q000166:cytology* / Q000502:physiology; D007700:Kinetics; D008564:Membrane Potentials; D008757:Methylglucosides / Q000378:metabolism; D009861:Onium Compounds / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D011865:Radioisotope Dilution Technique
chemical_list: D002250:Carbon Radioisotopes; D007202:Indicators and Reagents; D008757:Methylglucosides; D009861:Onium Compounds; D009943:Organophosphorus Compounds; C027020:methylglucoside; C013289:tetraphenylphosphonium