title: Characteristics of taurine transport in rat liver lysosomes.
abstract: Taurine (2-aminoethanesulfonic acid) is a unique sulfur amino acid derivative that has putative nutritional, osmoregulatory, and neuroregulatory roles and is highly concentrated within a variety of cells. The permeability of Percoll density gradient purified rat liver lysosomes to taurine was examined. Intralysosomal amino acid analysis showed trace levels of taurine compared to most other amino acids. Taurine uptake was Na(+)-independent, with an overshoot between 5-10 minutes. Trichloroacetic acid extraction studies and detergent lysis confirmed that free taurine accumulated in the lysosomal space. Kinetic studies revealed heterogeneous uptake with values for Km1 = 31 +/- 1.82 and Km2 greater than 198 +/- 10.2 mM. The uptake had a pH optimal of 6.5 and was stimulated by the potassium specific ionophore valinomycin. The exodus rate was fairly rapid, with a t1/2 of 5 minutes at 37 degrees C. Analog inhibition studies indicated substrate specificity similar to the plasma membrane beta-alanine carrier system, with inhibition by beta-alanine, hypotaurine, and taurine. alpha-Alanine, 2-methylaminoisobutyric acid (MeAIB), and threonine were poor inhibitors. No effects were observed with sucrose and the photoaffinity derivative of taurine NAP-taurine [N-(4-azido-2-nitrophenyl)-2-aminoethanesulfonate]. In summary, rat liver lysosomes possess a high Km system for taurine transport that is sensitive to changes in K+ gradient and perhaps valinomycin induced diffusional membrane potential. These features may enable lysosomes to adapt to changing intracellular concentrations of this osmotic regulatory substance.
authors.affiliation: Division of Medical Genetics, Harbor-UCLA Medical Center, Torrance 90502.
authors.forename: J V
authors.initials: JV
authors.lastname: Vadgama
authors.forename: K
authors.initials: K
authors.lastname: Chang
authors.forename: J D
authors.initials: JD
authors.lastname: Kopple
authors.forename: J M
authors.initials: JM
authors.lastname: Idriss
authors.forename: A J
authors.initials: AJ
authors.lastname: Jonas
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology; D005260:Female; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*; D008247:Lysosomes / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects; D008985:Monensin / Q000494:pharmacology; D009550:Nigericin / Q000494:pharmacology; D009994:Osmolar Concentration; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000494:pharmacology; D013379:Substrate Specificity; D013654:Taurine / Q000378:metabolism / Q000493:pharmacokinetics*; D013997:Time Factors; D014634:Valinomycin / Q000494:pharmacology
chemical_list: D013654:Taurine; D014634:Valinomycin; D000431:Ethanol; D000255:Adenosine Triphosphate; D008985:Monensin; D012964:Sodium; D009550:Nigericin