Processing of pro-opiomelanocortin-derived amidated joining peptide and glycine-extended precursor in monkey pituitary.
The molecular forms of proopiomelanocortin (POMC) derived amidated and C-terminal glycine-extended joining peptide from monkey (Macaca mulatta) pituitary were determined. The predominant forms of joining peptide found were the low molecular peptides POMC(76-105) and POMC(76-106), respectively. Significant amounts of N-terminally truncated POMC(78-105) and POMC(78-106) were also detected in the posterior-intermediate lobe. No N-terminal extended forms were detected. The relative amount of amidated joining peptide to total joining peptide was 6-35%. It is concluded that not only is the primary sequence of monkey and human POMC extremely conserved, but also the processing patterns are similar. The monkey therefore serves as a suitable model for studying regulation of the processing of POMC and the hypothalamus-pituitary-adrenal axis in man.
Fenger
M
M
Mental Health Research Institute, University of Michigan, Ann Arbor 48109.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005998:Glycine / Q000502:physiology*; D066298:In Vitro Techniques; D007700:Kinetics; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism*; D010902:Pituitary Gland / Q000378:metabolism*; D011333:Pro-Opiomelanocortin / Q000235:genetics / Q000378:metabolism*; D011863:Radioimmunoassay
D000596:Amino Acids; D010446:Peptide Fragments; D011333:Pro-Opiomelanocortin; C064114:pro-opiomelanocortin joining peptide(79-108); D005998:Glycine