Identification and expression of boar sperm proteins in the reproductive tract that interact with antibodies in serum from an infertile woman.
Serum designated as IS obtained from a young healthy infertile woman induced a head-to-head agglutination of ejaculated boar sperm. The immunoglobulin G (IgG) prepared from IS localized to the acrosomal region of the sperm head obtained from the corpus and cauda epididymis as determined by an indirect immunofluorescent method. The IgG interacted with a boar sperm protein with an estimated molecular weight of 45-kDa, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoretic (SDS-PAGE) immunoblotting technique. However, the IgG did not interact with proteins extracted from sperm obtained from the testis and caput epididymis or from non-gonadal tissues including liver, kidney, spleen, muscle and serum. The IgG interacted with additional proteins of about 75- and 38-kDa present in the corpus and cauda epididymal fluids but not those in the caput epididymal fluid. The staining intensity of the 75-kDa band was reduced and that of the 38-kDa was nullified with ejaculated seminal plasma proteins. The interacting proteins were adsorbed when chromatographed on Concanavalin A Sepharose column, suggesting that they are glycoproteins.
Haneji
T
T
Department of Anatomy, School of Medicine, Chiba University, Japan.
Nagano
T
T
Koide
S S
SS
D000372:Agglutination Tests; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004822:Epididymis / Q000276:immunology / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000276:immunology; D007247:Infertility, Female / Q000097:blood / Q000276:immunology*; D008297:Male; D014176:Protein Biosynthesis; D011506:Proteins / Q000276:immunology*; D013094:Spermatozoa / Q000276:immunology* / Q000378:metabolism; D013552:Swine; D013737:Testis / Q000276:immunology / Q000378:metabolism
D007074:Immunoglobulin G; D011506:Proteins