Studies of the proliferation and differentiation of immature myeloid cells in vitro: 4: Preculture proto-oncogene expression and the behaviour of myeloid leukemia cells in vitro.
Studies were conducted to determine the relationship between the pretherapy characteristics of leukemia cells and their behaviour during culture in vitro. Leukemia cells which proliferated well in vitro also proliferated well in vivo. Cells which manifested myeloid or monocytic differentiation in vivo tended to manifest differentiation along these lines in vitro. Cells which manifested high levels of expression of c-fms, c-fes, or triose phosphate isomerase prior to culture were likely to differentiate in vitro, with high levels of c-fes expression being related to myeloid maturation. These observations suggest that differentiation at the molecular level prior to culture is a requisite for leukemia cell differentiation in vitro. The same may be true for differentiation in vivo under the influence of exogenously administered agents such as cytotoxic chemotherapy or recombinant growth factors.
Yin
M Y
MY
Barrett Cancer Center, University of Cincinnati Medical Center, Ohio 45267-0502.
Gao
X Z
XZ
Wang
Z Q
ZQ
Preisler
H D
HD
D000328:Adult; D000368:Aged; D001853:Bone Marrow / Q000473:pathology; D002454:Cell Differentiation; D002455:Cell Division; D005260:Female; D015870:Gene Expression / Q000502:physiology; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009000:Monocytes; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D011519:Proto-Oncogenes; D011994:Recombinant Proteins / Q000494:pharmacology; D014305:Triose-Phosphate Isomerase / Q000378:metabolism; D014407:Tumor Cells, Cultured
D006133:Growth Substances; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D011994:Recombinant Proteins; D014305:Triose-Phosphate Isomerase