[The effect of the carbon sources and calcium ions on the activity of thermostable alpha-amylase in Bacillus sp. 86].
Amylolytic activity of Bacillus sp. 86 thermophilic strain was studied as affected by carbon sources. Glucose and saccharose being added to the nutrition medium with baker's yeast as the basic source of carbon the activity of alpha-amylase decreased. Introduction of native or soluble starch to the medium promoted a sharp increase in the enzymic activity. Bacillus sp. 86 being periodically cultivated in the optimized medium containing 4% of soluble starch and 0.5% of CaCl2, an eight-fold increase of amylolytic activity of cultural liquid was achieved as compared to the enzyme activity in the primary medium.
Kichakova
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D001407:Bacillus / Q000201:enzymology / Q000302:isolation & purification; D002118:Calcium / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism*; D003470:Culture Media; D004795:Enzyme Stability; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D012988:Soil Microbiology; D000516:alpha-Amylases / Q000378:metabolism*
D003470:Culture Media; D002244:Carbon; D000516:alpha-Amylases; D002118:Calcium
Vliianie istochnikov ugleroda i ionov kal'tsiia na aktivnost' termostabil'noĭ al'fa-amilazy Bacillus sp. 86.