[The regulation of DNA repair in mammalian cells. I. The repair of DNA radiation damages in Swiss 3T6 mouse cells under the action of the epidermal growth factor and insulin].
Methods of centrifugation in alkaline sucrose gradients as well as alkaline and neutral elution on filters were used to show a significant reduction in the rate of both single- and double-strand DNA breaks in the quiescent mouse Swiss 3T6 cell culture as compared to the proliferating one. The low efficiency of repair of single-strand DNA breaks in quiescent cells may result from a nearly complete absence of the fast repair of DNA lesions during the first minutes of postradiation incubation. The epidermal growth factor in combination with insulin (no other serum component present) leads to a recovery of the repair process. The stimulating effect of mitogens on the repair of both single- and double-strand DNA breaks allows to suggest that similar factors may be responsible for these recovery processes.
Bil'din
V N
VN
Seregina
T B
TB
Zhestianikov
V D
VD
D000818:Animals; D002461:Cell Line, Transformed; D002478:Cells, Cultured / Q000187:drug effects / Q000528:radiation effects; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects / Q000528:radiation effects*; D004249:DNA Damage / Q000187:drug effects*; D004260:DNA Repair / Q000187:drug effects*; D004277:DNA, Single-Stranded / Q000096:biosynthesis / Q000187:drug effects / Q000528:radiation effects*; D004815:Epidermal Growth Factor / Q000302:isolation & purification / Q000494:pharmacology*; D005720:Gamma Rays; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D051379:Mice; D013997:Time Factors
D004277:DNA, Single-Stranded; D007328:Insulin; D004815:Epidermal Growth Factor; D004247:DNA
Reguliatsiia reparatsii DNK v kletkakh mlekopitaiushchikh. I. Reparatsiia radiatsionnykh povrezhdeniĭ DNK v myshinykh kletkakh Swiss 3T6 pri deĭstvii épidermal'nogo faktora rosta i insulina.