Intracellular analysis of effects of phencyclidine on rat neostriatal neurons recorded in vitro.
The effects of phencyclidine (PCP) on the synaptic and direct membrane properties of neostriatal neurons were assessed using intracellular recordings from 62 neurons in rat neostriatal slices. Electrophysiological measurements were obtained before, during and after bath application of PCP. At all concentrations (1-300 microM) PCP raised current thresholds for evoking synaptic potentials and reduced excitability (the firing rate of directly evoked action potentials). In a smaller number of cells PCP (50-300 microM) increased input resistance. These effects were long-lasting and neurons did not show reversal with washes as long as 75 min. Some neurons were injected with biocytin for identification. All were medium-sized spiny type I neurons typical of rodent neostriatum.
Bailey
C S
CS
Mental Retardation Research Center, University of California, Los Angeles 90024-1759.
Howard
S G
SG
Levine
M S
MS
D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects / Q000502:physiology*; D004553:Electric Conductivity; D004558:Electric Stimulation; D005071:Evoked Potentials / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D010622:Phencyclidine / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013569:Synapses / Q000187:drug effects / Q000502:physiology
D010622:Phencyclidine