The interaction of bile salt micelles with the dansyltyrosine derivatives of porcine colipase.
The interaction of bile salt micelles with the tyrosines of pancreatic colipase was assessed by steady-state and time-resolved fluorescence techniques. Dansyltyrosine fluorescence showed that Tyr-55 was located in the proposed interface recognition site. In support of this claim was a 70 nm blue shift and 4.3-fold quantum yield increase in emission spectrum due to taurodeoxycholate (TDOC) micelle-complex formation. Complex formation also caused a shift in the center of the major lifetime distribution from 11.7 to 15.1 ns, and more than doubled the polarization and anisotropy decay parameters. These data supported an earlier model of colipase-micelle binding that suggested that Tyr-55 was inserted into the interior of the TDOC micelle upon binding (J.C. McIntyre, P. Hundley and W.D. Behnke, Biochem. J. 245 (1987) 821). Identical experiments on a DNS-Tyr-59 derivative of colipase showed that Tyr-59 did not specifically interact with micelles. Moreover, acrylamide quenching data suggest an alteration in the protein environment surrounding DNS-Tyr-59 such that during complex formation, the efficiency of quenching of DNS-Tyr-59 increases.
McIntyre
J C
JC
Department of Molecular Genetics, Biochemistry and Microbiology, College of Medicine, University of Cincinnati, OH 45267.
Schroeder
F
F
Behnke
W D
WD
D000178:Acrylamides; D000818:Animals; D001647:Bile Acids and Salts / Q000737:chemistry*; D003089:Colipases / Q000737:chemistry*; D003619:Dansyl Compounds / Q000737:chemistry*; D008823:Micelles; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013552:Swine; D014443:Tyrosine / Q000737:chemistry*
D000178:Acrylamides; D001647:Bile Acids and Salts; D003089:Colipases; D003619:Dansyl Compounds; D008823:Micelles; D014443:Tyrosine