title: The effect of DPT inoculation on the hormonal control of glucose homeostasis in children recovered from malnutrition.
abstract: The effect of a controlled stress (DPT inoculation) on the hormonal control of glucose homeostasis was investigated in children nutritionally rehabilitated from severe malnutrition. The age range of the 15 children studied was 6-26 months. Plasma insulin (INS), growth hormone (GH) and interleukin-1 (IL-1) were measured by radioimmunoassay; plasma glucose (GLU) by a glucose oxidase method; and red cell insulin binding (%SB) was determined, using A-14 monoiodinated insulin. Measurements were made on two occasions: (T-0) at 10 a.m., 12 hr before DPT inoculation, and (T-36) 36 hr. after inoculation. On both occasions, 4 hr post-prandial blood samples were used, and the mean body temperature (T) on the day of the test was determined. Red cell insulin binding (%SB) was significantly higher at T-36 than at T-0 (16.8 +/- 1.7 vs 12.1 +/- 1.2 (14), p = 0.005). (Results were expressed as mean +/- SEM, numbers of paired observations in parentheses). The higher %SB after DPT was accompanied by an increase in the number of receptor sites (S) (29.05 +/- 6.5 vs 15.6 +/- 2.5 (14), p = 0.025). However, insulin receptor affinity (K x 10(9) M-1) was decreased (0.7 +/- 0.1 vs 1.5 +/- 0.3 (14), p = 0.008). There were no significant differences in the plasma levels of insulin, glucose and interleukin-1, but plasma growth hormone (microU/ml) was increased after DPT, (18.0 +/- 3.0 vs. 11.5 +/- 1.2 (13), p = 0.04). Body temperature (degree C) was also significantly increased after DPT, (99.6 +/- 0.4 vs. 98.3 +/- 0.2 (14), p = 0.006).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Tropical Metabolism Research Unit, U.W.I., Jamaica.
authors.forename: H M
authors.initials: HM
authors.lastname: Payne-Robinson
authors.forename: J F
authors.initials: JF
authors.lastname: Doherty
authors.forename: M H
authors.initials: MH
authors.lastname: Golden
authors.forename: D T
authors.initials: DT
authors.lastname: Simeon
mesh_terms: D001786:Blood Glucose / Q000378:metabolism*; D001831:Body Temperature / Q000187:drug effects; D015362:Child Nutrition Disorders / Q000097:blood*; D002675:Child, Preschool; D015721:Diphtheria-Tetanus-Pertussis Vaccine / Q000009:adverse effects*; D013006:Growth Hormone / Q000097:blood; D006706:Homeostasis / Q000187:drug effects*; D006728:Hormones / Q000097:blood*; D006801:Humans; D007223:Infant; D007328:Insulin / Q000097:blood; D007375:Interleukin-1 / Q000097:blood; D011863:Radioimmunoassay
chemical_list: D001786:Blood Glucose; D015721:Diphtheria-Tetanus-Pertussis Vaccine; D006728:Hormones; D007328:Insulin; D007375:Interleukin-1; D013006:Growth Hormone