Single-copy flanking sequences in human histone gene clusters map to chromosomes 1 and 6.
Two single-copy sequences flanking two different human histone gene clusters were used as probes to map these clusters by in situ hybridization. pFF435B, a unique sequence subclone derived from a lambda genomic clone (lambda HHG55) containing H2A, H2B, H3, and H4 genes, mapped to chromosome 1q21 (chi 2 = 120.99, P less than 0.001). pST519E, a single-copy sequence derived from a lambda genomic clone (lambda HHG17) containing only H3 and H4 genes, mapped to chromosome 6p21 (chi 2 = 112.62, P less than 0.001). These findings agree with previous assignments of human histone genes to chromosomes 1 and 6 and demonstrate that the single-copy flanking sequences in different human histone gene clusters are unique for different chromosomes.
Allen
B S
BS
Department of Biochemistry and Molecular Biology, University of Florida, Gainesville 32610.
Stein
J L
JL
Stein
G S
GS
Ostrer
H
H
D002878:Chromosomes, Human, Pair 1; D002896:Chromosomes, Human, Pair 6; D006657:Histones / Q000235:genetics*; D006801:Humans; D007438:Introns; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping
D006657:Histones