The role of endothelial second messenger's-generating system in the pathogenesis of brain oedema.
The role of second messengers in the regulation of protein phosphorylation was studied in microvessels isolated from rat cerebral cortex. Calcium-calmodulin (CAM)-, Ca2+/phospholipid (PK C)-, cyclic GMP (cGMP)-, and cyclic AMP (cAMP)-dependent protein kinases were detected. Autophosphorylation of both the alpha- and beta-subunits of CAM-dependent protein kinase and the proteolytic fragment of the PK C enzyme was also detected. In other experiments, the effect of the protein kinase C enzyme inhibitor H-7 was examined on the brain oedema formation evoked by bilateral occlusion of the common carotid arteries in Sprague-Dawley rats of CFY strain.
Joó
F
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Laboratory of Molecular Neurobiology, Biological Research Center, Szeged, Hungary.
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D001929:Brain Edema / Q000209:etiology* / Q000517:prevention & control; D002560:Cerebrovascular Circulation; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000502:physiology*; D005260:Female; D007546:Isoquinolines / Q000494:pharmacology; D010766:Phosphorylation; D010879:Piperazines / Q000494:pharmacology; D047428:Protein Kinase Inhibitors; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D015290:Second Messenger Systems
D007546:Isoquinolines; D010879:Piperazines; D047428:Protein Kinase Inhibitors; D011506:Proteins; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine