HLA-DR/Dw matching by PCR fingerprinting: the origin of PCR fingerprints and further applications.
Polymerase chain reaction (PCR) fingerprinting, a new method for the rapid matching of HLA-Dr/Dw allotypes, involves the visual comparison of polymorphic HLA-DRB gene second exon PCR products, resolved in non-denaturing polyacrylamide gels (Bidwell & Hui, 1990). We show here that the satellite DNA bands within PCR fingerprints originate by heteroduplex formation between heterologous DNAs co-amplified by a common PCR primer set. We also present two further applications of the technique which permit discrimination between unrelated HLA-DR/Dw allotypes with similar PCR fingerprints.
Wood
N A
NA
Molecular Genetics Laboratory, U.K. Transplant Service, Bristol.
Clay
T M
TM
Bidwell
J L
JL
D016172:DNA Fingerprinting / Q000379:methods*; D006684:HLA-DR Antigens / Q000145:classification / Q000235:genetics*; D006579:Heterozygote; D006650:Histocompatibility Testing / Q000379:methods; D006720:Homozygote; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
D006684:HLA-DR Antigens