Demonstration by confocal laserscanning microscopy of invasive potential with Bacteroides fragilis.
The confocal laserscanning microscopy (CLSM) system provides a stereoscopic view of an object. By this system the penetration of B. fragilis into HeLa cells was observed. The intensity of contact is highlighted with time. The CLSM system consolidates the recently described fluorescence technique to test invasive potential. This work purports that certain gram-negative anaerobes should be considered for invasiveness.
Goldner
M
M
Unité des Anaérobies et Station Centrale de Microscopie Electronique, Institut Pasteur, Paris.
Coquis-Rondon
M
M
Carlier
J P
JP
D001422:Bacterial Adhesion; D001441:Bacteroides fragilis / Q000472:pathogenicity* / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D008853:Microscopy; D014774:Virulence