title: Ontogeny of mitochondrial calcium transport in spontaneously hypertensive (SHR) and WKY rats.
abstract: The current studies were designed to investigate calcium uptake by intestinal jejunal sacs as well as in intestinal mitochondria of spontaneously hypertensive rats and their genetically matched WKY control rats. Kinetics of jejunal calcium uptake by jejunal sacs of adult SHR and WKY rats showed a significant decrease in Vmax of calcium uptake in SHR (227 +/- 24 versus 423 +/- 22 nmol.g tissue-1.3 min-1) compared to WKY rats P less than 0.001. To explore the intracellular handling of calcium by the intestinal mitochondria, calcium uptake was characterized by intestinal mitochondria before (suckling and weanling periods) and after (adult period) development of hypertension. Calcium uptake by intestinal mitochondria was driven by ATP in the presence of succinate as a respiratory substrate. Calcium uptake was stimulated several fold by the presence of ATP compared to no ATP conditions. Maximal calcium uptake occurred between 15-30 min and was significantly greater in adult SHR and WKY rats compared to corresponding values in weanling and suckling rats. Maximal ATP dependent calcium uptake in adult, weanling and suckling WKY rats was significantly greater compared to corresponding mean values in each age group in SHR (P less than 0.001). Oligomycin (10 micrograms/mg protein) inhibited calcium uptake partially. Ruthenium red (0.25 microM), 1 mM sodium azide and 0.5 mM dinitrophenol inhibited calcium uptake by more than 80% in both SHR and WKY rats. Kinetic parameters for ATP stimulated calcium uptake at 10 s revealed a Vmax of 0.56 +/- 0.6, 3.46 +/- 0.23 and 3.95 +/- 0.52 nmol/mg protein/10 s in suckling, weanling and adult WKY rats.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN 37232.
authors.forename: N
authors.initials: N
authors.lastname: Arab
authors.forename: S H
authors.initials: SH
authors.lastname: Shibata
authors.forename: F K
authors.initials: FK
authors.lastname: Ghishan
mesh_terms: D000367:Age Factors; D000818:Animals; D000833:Animals, Suckling; D001693:Biological Transport, Active / Q000235:genetics; D002118:Calcium / Q000378:metabolism*; D046508:Culture Techniques; D004195:Disease Models, Animal; D006973:Hypertension / Q000235:genetics / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D013997:Time Factors; D014886:Weaning
chemical_list: D002118:Calcium