Urinary excretion of differently sized polyethylene glycols after intravenous administration in uremic and control rats: effects of low- and high-protein diets.
The intestine constitutes a barrier towards potentially harmful agents in the intestinal lumen. Different-sized polyethylene glycols (PEGs; range 326-1,1162 Da) have been used to study the intestinal permeability properties in 5/6-nephrectomized rats on either a high- (22%) or low-protein (8%) diet. PEGs were administered intravenously, and the urinary recovery was measured. The 24-hour urinary recovery of PEGs was significantly reduced in the uremic groups. The ratios between different sizes of PEGs, indicating a size-selective escape of molecules from blood, were decreased in the uremic groups. The urinary recovery was in general increased in the control group on the high-protein diet compared to the control group on the low-protein diet. The results provide evidence for an increased permeability of larger PEGs (range 634-1,162 Da) in uremic rats and that the protein content of the diet might affect the permeability properties.
Magnusson
M
M
Department of Nephrology, University of Linköping, Sweden.
Magnusson
K E
KE
Sundqvist
T
T
Denneberg
T
T
D000818:Animals; D004044:Dietary Proteins / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007275:Injections, Intravenous; D007668:Kidney / Q000502:physiology / Q000503:physiopathology; D008297:Male; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014511:Uremia / Q000652:urine*
D004044:Dietary Proteins; D011092:Polyethylene Glycols