title: Affinity purification of transcription factor IIA from HeLa cell nuclear extracts.
abstract: One of the general transcription factors, TFIIA, was purified to homogeneity from HeLa cell nuclear extracts by yeast TFIID affinity chromatography. Human TFIIA had a molecular weight of approximately 38 kd. It was able to associate with the complex formed by yeast TFIID and the TATA elements of the adenovirus E4 and ML promoters, and the HSP70 promoter. The association extended the protected region on each TATA element by yeast TFIID from DNase I digestion. Affinity-purified TFIIA was also able to stimulate transcription from the E4 and ML promoters in in vitro reconstituted systems.
authors.affiliation: Department of Bacteriology, University of Tokyo Faculty of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Usuda
authors.forename: A
authors.initials: A
authors.lastname: Kubota
authors.forename: A J
authors.initials: AJ
authors.lastname: Berk
authors.forename: H
authors.initials: H
authors.lastname: Handa
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D001483:Base Sequence; D002846:Chromatography, Affinity; D016172:DNA Fingerprinting; D005814:Genes, Viral; D006367:HeLa Cells; D006360:Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013379:Substrate Specificity; D016385:TATA Box; D035561:Transcription Factor TFIIA; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D006360:Heat-Shock Proteins; D035561:Transcription Factor TFIIA; D035362:Transcription Factor TFIID; D014157:Transcription Factors