Characterization of insulin receptors in primary cultures of quail (Coturnix coturnix japonica) oviduct cells. The level of insulin receptor is regulated by steroid and peptide hormones.
1. We have characterized the insulin receptor in primary cultured quail oviduct cells and examined the hormonal regulation of its level. 2. We have also shown the recycling pathway of insulin receptors in the cultured cells using specific inhibitors (tunicamycin, chloroquine, monensin, and brefeldin A). 3. Our data suggest that glucocorticoids play important physiological roles in egg-white protein synthesis through increasing the number of insulin receptors and insulin through enhancing the transport of amino acids.
Kato
S
S
Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Japan.
Itoh
S
S
Miura
Y
Y
Naito
H
H
Noguchi
T
T
D000621:Aminoisobutyric Acids / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002478:Cells, Cultured; D003370:Coturnix; D003907:Dexamethasone / Q000494:pharmacology*; D015536:Down-Regulation; D005947:Glucose / Q000378:metabolism; D006031:Glycosylation; D006728:Hormones / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism / Q000494:pharmacology*; D010057:Oviducts / Q000166:cytology / Q000378:metabolism*; D014176:Protein Biosynthesis; D011972:Receptor, Insulin / Q000378:metabolism*; D015854:Up-Regulation
D000621:Aminoisobutyric Acids; D006728:Hormones; D007328:Insulin; D003907:Dexamethasone; D011972:Receptor, Insulin; D005947:Glucose