In situ localization of NF-H neurofilament subunit mRNAs in rat brain.
The expression of NF-H neurofilament subunit mRNAs was investigated in the rat brain at different ontogenic stages. The levels of NF-H mRNAs vary 15-fold among brain regions with the highest level in the brainstem. In situ localization studies revealed that the NF-H mRNAs are mainly concentrated in the brainstem motoneuron nuclei. By increasing the sensitivity of the hybridization method, NF-H mRNAs could also be localized in neurons present in the cortex, thalamus and hippocampus areas. Minor amounts of NF-H mRNAs were already detected at 17-day embryonic stages.
Roussel
G
G
Centre de Neurochimie du CNRS and U44 de l'INSERM, Strasbourg, France.
Felix
J M
JM
Dautigny
A
A
Pham-Dinh
D
D
Hindelang
C
C
Jollès
P
P
Nussbaum
J L
JL
D000818:Animals; D015152:Blotting, Northern; D001923:Brain Chemistry; D001933:Brain Stem / Q000033:anatomy & histology / Q000378:metabolism; D002540:Cerebral Cortex / Q000033:anatomy & histology / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006624:Hippocampus / Q000033:anatomy & histology / Q000378:metabolism; D007382:Intermediate Filaments / Q000378:metabolism*; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis* / Q000737:chemistry; D051381:Rats; D013788:Thalamus / Q000033:anatomy & histology / Q000378:metabolism
D012333:RNA, Messenger