Morphometric study of immunoreactive GH-cells of the adenohypophysis of rats treated with Met-enkephalin.
Morphological and morphometric changes were found in adenohypophyseal GH immunoreactive cells after intraventricular administration of Met-enkephalin to adult rats of both sexes. Morphologically, following Met-enkephalin treatment, the males showed cells stained intensely and homogeneously in their cytoplasm, while the females showed cells with a weaker degree of staining and a cytoplasm with a granular aspect; previous administration of naloxone prevented the changes observed in the males and intensified the reaction in the females. Morphometrically, in all the groups of animals studied, the males showed a greater cellular area than the females, accompanied by a decrease in the cytoplasmic area but without significant differences in the nuclear area. Met-enkephalin in both sexes produced an increase in cellular area, accompanied by a significant increase in the cytoplasmic area and, although less manifest, in the nuclear area. Neither kind of change appeared when naloxone was administered prior to Met-enkephalin treatment.
Carretero
J
J
Department of Human Anatomy and Histology, Faculty of Medicine, University of Salamanca, Spain.
Sanchez
D
D
Sanchez
F
F
Montero
M
M
Blanco
E
E
Gonzalez
R
R
Vazquez
R
R
D000818:Animals; D003593:Cytoplasm / Q000648:ultrastructure; D004744:Enkephalin, Methionine / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D013006:Growth Hormone / Q000096:biosynthesis / Q000502:physiology*; D007150:Immunohistochemistry; D007276:Injections, Intraventricular; D008297:Male; D009270:Naloxone / Q000494:pharmacology; D010903:Pituitary Gland, Anterior / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012737:Sex Factors
D009270:Naloxone; D004744:Enkephalin, Methionine; D013006:Growth Hormone