[Immunohistochemical study of the surface membrane on a intra-ocular lens].
Surface membranes of intraocular lenses implanted in three rabbit, were studied immunohistochemically with anti-rabbit fibronectin serum and anti-rabbit whole serum antiserum. The specimens were embedded in an epoxy resin and cut at 1 micron. Before the immunostaining procedure, the sections were placed in a solution of saturated NaOH in absolute ethanol to remove the epoxy resin. Immunostaining procedure was performed by routine avidin-biotin-peroxidase technique. The surface membranes showed positive reactions against both anti-sera. These results indicated that the surface membrane of implanted intraocular lenses was composed of fibronectin with some other serum proteins.
Sakamoto
T
T
Department of Pathology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Ishibashi
T
T
Sugai
S
S
Ohnishi
Y
Y
Fukushima
M
M
Sueishi
K
K
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D007150:Immunohistochemistry; D007910:Lenses, Intraocular; D008566:Membranes / Q000378:metabolism; D011817:Rabbits
D001798:Blood Proteins; D005353:Fibronectins