title: [Oxidase activity of horseradish peroxidase in oxidation of the bilirubin-albumin complex].
abstract: The catalytic activity of horseradish peroxidase was investigated upon oxidation of the bilirubin-albumin complex (10(-5)-10(-4) M; BR: SA = 1: 1.5 mol/mol) by dissolved oxygen to biliverdin and other components. The process can be described by the Michaelis-Menten equation with Km = 14.4 microM and Vmax = 0.12 microM/min, if HRP = 3.1 x 10(-8) M. Oxidation is supposed to proceed in the triple complex--peroxidase: albumin: bilirubin.
authors.forename: O N
authors.initials: ON
authors.lastname: Zefirova
authors.forename: G A
authors.initials: GA
authors.lastname: Sytov
authors.forename: V A
authors.initials: VA
authors.lastname: Postnikov
authors.forename: L I
authors.initials: LI
authors.lastname: Valuev
authors.forename: A
authors.initials: A
authors.lastname: Plate
mesh_terms: D000818:Animals; D001663:Bilirubin / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D002417:Cattle; D006735:Horseradish Peroxidase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D010084:Oxidation-Reduction; D012709:Serum Albumin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D012709:Serum Albumin; D006735:Horseradish Peroxidase; D002374:Catalase; D001663:Bilirubin
vernacular_title: Oksidaznaia aktivnost' peroksidazy khrena v okislenii kompleksa bilirubin-al'bumin.