Recovery of octyl beta-glucoside from detergent/protein mixtures.
Octyl beta-glucoside is a nonionic detergent which is useful in the purification and characterization of membrane proteins. However, the cost of this reagent is prohibitive for large-scale protein purifications. A simple procedure to recover pure octyl beta-glucoside from mixtures of detergents with proteins and buffer salts is described.
Kobs
S F
SF
Department of Chemistry, Ohio State University, Columbus 43210.
D002021:Buffers; D003902:Detergents / Q000302:isolation & purification*; D005960:Glucosides / Q000302:isolation & purification*; D008565:Membrane Proteins / Q000302:isolation & purification*
D002021:Buffers; D003902:Detergents; D005960:Glucosides; D008565:Membrane Proteins; C018619:octyl-beta-D-glucoside