Capillary electrophoresis of amino sugars with laser-induced fluorescence detection.
3-(4-Carboxybenzoyl)-2-quinolinecarboxaldehyde has been utilized as a precolumn derivatization agent for various amino sugars. Constituents of various biological mixtures can be converted to highly fluorescent isoindole derivatives, separated by high-performance capillary electrophoresis and determined at attomole (10(-18) mol) levels by a laser-induced fluorescence detector. This method has been applied to the analysis of monosaccharides and acid-hydrolyzed polysaccharides. Carbohydrate moieties derived from a glycoprotein were also tagged and determined.
Liu
J P
JP
Department of Chemistry, Indiana University, Bloomington 47405.
Shirota
O
O
Novotny
M
M
D000606:Amino Sugars / Q000032:analysis* / Q000737:chemistry; D004586:Electrophoresis / Q000379:methods*; D006868:Hydrolysis; D007834:Lasers; D013050:Spectrometry, Fluorescence
D000606:Amino Sugars