title: Agglutination of human O erythrocytes by influenza A(H1N1) viruses freshly isolated from patients.
abstract: The hemagglutinin titers of 10 influenza A (H1N1) viruses were examined using the erythrocytes of several species. Human O erythrocytes showed the highest agglutination titer to the viruses, whereas chicken erythrocytes showed a low titer. These findings were noted for at least 10 passages by serial dilutions of the viruses in Madin-Darby canine kidney (MDCK) cells. All influenza A(H1N1) viruses, plaque-cloned directly from throat-washing specimens of patients, also agglutinated human O but not chicken erythrocytes. The results of a hemadsorption test indicated that chicken erythrocytes possess less affinity to MDCK cells infected with the A/Osaka City/2/88(H1N1) stain than to those infected with the A/Yamagata/120/86(H1N1) strain which is used as an inactivated influenza vaccine in Japan. However, there were no significant differences between the A/Osaka City/2/88 and the A/Yamagata/120/86 strains in the hemagglutination inhibition test. Since human O erythrocytes have high agglutination activity to influenza A(H1N1) and also to A(H3N2) and B viruses in MDCK cells, these erythrocytes may be useful for the serological diagnosis of influenza.
authors.affiliation: Department of Virology, Osaka City Institute of Public Health and Environmental Sciences, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Murakami
authors.forename: K
authors.initials: K
authors.lastname: Haruki
authors.forename: Y
authors.initials: Y
authors.lastname: Seto
authors.forename: T
authors.initials: T
authors.lastname: Kimura
authors.forename: S
authors.initials: S
authors.lastname: Minoshiro
authors.forename: K
authors.initials: K
authors.lastname: Shibe
mesh_terms: D000328:Adult; D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D002460:Cell Line; D002645:Chickens; D002648:Child; D004912:Erythrocytes / Q000276:immunology; D006168:Guinea Pigs; D006382:Hemadsorption; D006386:Hemagglutination Tests; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype; D009980:Influenza A virus / Q000276:immunology*; D007251:Influenza, Human / Q000175:diagnosis*; D010948:Viral Plaque Assay
chemical_list: D000956:Antigens, Viral