title: [Identification of myosin light chain 1 from human heart ventricles on two-dimensional electrophoretograms using monoclonal antibodies].
abstract: Two-dimensional electrophoresis performed according to O'Farrell was used to isolate the myosin light chain I from human heart ventricles. This chain was further used to obtain hybridomas able to secrete monoclonal antibodies. This method permits to increase the yield of monoclonal antibody-secreting hydridomas and to spare effort with regard to cultivation and testing of experimental samples.
authors.forename: L I
authors.initials: LI
authors.lastname: Kovalev
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Volgin
authors.forename: A L
authors.initials: AL
authors.lastname: Konorova
authors.forename: M N
authors.initials: MN
authors.lastname: Tsvetkova
authors.forename: E V
authors.initials: EV
authors.lastname: Puliaeva
authors.forename: L A
authors.initials: LA
authors.lastname: Pevnitskiĭ
authors.forename: S S
authors.initials: SS
authors.lastname: Shishkin
mesh_terms: D000911:Antibodies, Monoclonal / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D006352:Heart Ventricles / Q000737:chemistry*; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D009218:Myosins / Q000302:isolation & purification*
chemical_list: D000911:Antibodies, Monoclonal; D009218:Myosins
vernacular_title: Identifikatsiia legkoĭ tsepi 1 miozina iz zheludochkov serdtsa cheloveka na dvumernykh élektroforegrammakh s pomoshch'iu monoklonal'nykh antitel.