[The characteristics of the ultrastructural organization of the apical plasma membrane of epithelial cells secreting hydrogen ions].
The analysis of ultrastructural characteristics of mitochondria-rich cells of the frog urinary bladder with the aid of three electron microscopic methods (ultrathin sections, scanning electron microscopy, freeze-fracture) has been done. The inverted distribution of globular intramembrane particles (IMP) in apical membranes reflecting their low water permeability has been shown. The typical feature of plasma membranes of mitochondria-rich cells is the presence of rod-shaped IMP on the P-face of the apical membrane and complementary pits on the EF. There is a correlation between the quantity of rod-shaped IMP and the rate of ionic transport. The analysis of cholesterol contents in plasma membranes of epithelial cells of the frog urinary bladder has shown that the apical membranes of mitochondria-rich cells contain more cholesterol than those of granular cells; the great pat of cholesterol is localized in the cytoplasmic leaflet.
Snigirevskaia
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Kever
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Komissarchik
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IaIu
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D004848:Epithelium / Q000378:metabolism / Q000648:ultrastructure; D005614:Freeze Fracturing; D006859:Hydrogen / Q000502:physiology*; D066298:In Vitro Techniques; D007477:Ions; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008928:Mitochondria / Q000378:metabolism / Q000648:ultrastructure; D001743:Urinary Bladder / Q000378:metabolism / Q000648:ultrastructure*
D007477:Ions; D006859:Hydrogen
Osobennosti ul'trastrukturnoĭ organizatsii apikal'noĭ plazmaticheskoĭ membrany épitelial'nykh kletok, sekretiruiushchikh iony vodoroda.