Reversed-phase chromatography of Escherichia coli ribosomal proteins. Correlation of retention time with chain length and hydrophobicity.
The elution times of 52 bacterial ribosomal proteins from a C4 reversed-phase column have been predicted. The prediction is based upon the use of the hydrophobicity coefficients for the protein amino acid content as defined by Guo et al. [J. Chromatogr., 359 (1986) 499-518]. A strong positive correlation was observed when the difference between predicted and observed protein retention time was plotted against the product of net hydrophobicity and natural log of protein chain length. Observations with this class of related proteins strengthens and extends the observations of Mant et al. [J. Chromatogr., 476 (1989) 363-375]. Observed deviations from predicted chromatographic behavior can be explained for several proteins which elute as dimers or which have modified amino acid residues.
Champney
W S
WS
Department of Biochemistry, College of Medicine, East Tennessee State University, Johnson City 37614.
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004926:Escherichia coli / Q000032:analysis*; D046911:Macromolecular Substances; D012269:Ribosomal Proteins / Q000737:chemistry / Q000302:isolation & purification*
D046911:Macromolecular Substances; D012269:Ribosomal Proteins