Halobacterium halobium Mn-SOD gene: archaebacterial and eubacterial features.
A 1.8 kb PstI fragment from Halobacterium halobium DNA was found to hybridize to synthetic oligonucleotide probes constructed by using the sequence of the N-terminus of a Mn-containing superoxide dismutase purified from H. halobium. The entire insert containing a 600-bp coding sequence for Mn-SOD and its 5' and 3' flanking regions was sequenced. The derived amino acid sequence of the structural gene showed a similarity to other manganic and iron-containing superoxide dismutases in normally conserved regions. Primer extension analysis of the H. halobium Mn-SOD mRNA showed that gene transcription begins 14 bases upstream of the translational start. A Shine-Dalgarno sequence and archaebacterial consensus promoter sequences were observed. Several other promoter and terminator nucleotide sequences homologous to prokaryotic and eukaryotic organisms were found.
Salin
M L
ML
Department of Biochemistry and Molecular Biology, Mississippi State University, MS 39762.
Duke
M V
MV
Ma
D P
DP
Boyle
J A
JA
D000595:Amino Acid Sequence; D001419:Bacteria / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D005798:Genes, Bacterial; D006217:Halobacterium / Q000201:enzymology / Q000235:genetics*; D006801:Humans; D008345:Manganese; D008969:Molecular Sequence Data; D010802:Phylogeny; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000235:genetics*
D001426:Bacterial Proteins; D008345:Manganese; D013482:Superoxide Dismutase