Cholic acid and chenodeoxycholic acid transport in the hepatic acinus in rats. Effect of necrosis of zone 3 induced by bromobenzene.
The transport of cholic acid (CA) and chenodeoxycholic acid (CDC) and their influence on bile formation was investigated in rats treated with bromobenzene (BZ), a toxicant which selectively destroys zone 3 of the hepatic acinus. The necrosis equals 27-31% of the acinus cells. The absence of zone 3 in rats reduced the secretory rate maximum of CA and CDC by 18% (NS) and 25% (p less than 0.05), respectively. The maximum bile flow was not different from control during CA infusion but was lower during CDC infusion in BZ-treated animals. Although the bile acid concentration was lower in BZ-treated rats, only values obtained during the basal period and the beginning of the infusion reached the level of statistically significant difference. The bile salt-independent flow (BSIF) was not affected by the absence of zone 3. Our data suggest that zones 1 and 2 of the hepatic acinus can compensate for the secretion of CA and elaboration of BSIF when zone 3 is destroyed. However, necrosis of zone 3 reduces CDC secretion. Thus, the capacity for bile acid transport of the hepatocytes of different zones in the hepatic acinus may differ according to the circulating bile acid.
Dionne
S
S
Pediatric Research Center, Hôpital Sainte-Justine, Montreal, Quebec, Canada.
Russo
P
P
Tuchweber
B
B
Plaa
G L
GL
Yousef
I M
IM
D000818:Animals; D001646:Bile / Q000378:metabolism; D001692:Biological Transport / Q000502:physiology; D001969:Bromobenzenes; D002635:Chenodeoxycholic Acid / Q000378:metabolism*; D019826:Cholic Acid; D002793:Cholic Acids / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009336:Necrosis; D051381:Rats; D011919:Rats, Inbred Strains; D012044:Regression Analysis
D001969:Bromobenzenes; D002793:Cholic Acids; D002635:Chenodeoxycholic Acid; C032036:bromobenzene; D019826:Cholic Acid