Assignment of allotypes G1m(a+) and G1m(a-) at the genomic level by polymerase chain reaction analysis.
A method of assignment of the human immunoglobulin allotypes G1m(a+) and G1m(a-) without the use of serological reagents is described. It is based upon oligonucleotide-directed enzymatic amplification of genomic segments encoding CH3 of gamma chains, followed by dot-blot hybridization of radioactively labelled oligonucleotides to the amplified DNA. The method was used to classify the immunoglobulin allotypes of 11 persons, six G1m(a+) and five G1m(a-), and the resultant classification agreed completely with that of classical serological typing.
Grubb
R
R
Department of Medical Microbiology, University of Lund, Sweden.
Abrahamson
M
M
Grubb
A
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D005803:Genes, Immunoglobulin; D006801:Humans; D007129:Immunoglobulin Gm Allotypes / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction
D007129:Immunoglobulin Gm Allotypes