Mass spectrometric determination of isotopically labeled tyrosines and tryptophans in photosynthetic reaction centers of Rhodobacter sphaeroides R-26.
A synthetic medium for growing Rhodobacter sphaeroides R-26 is developed. This medium opened the way to the preparation of photosynthetic reaction centers incorporated with L-[4'-13C]tyrosine or L-[1'-15N]tryptophan. Gas chromatography combined with mass spectroscopy was used to estimate the metabolic incorporation of the labeled amino acid into the protein. Conditions were found for near-quantitative incorporation of labeled aromatic amino acids into the reaction center.
Raap
J
J
Gorlaeus Laboratories, University of Leiden, The Netherlands.
Winkel
C
C
de Wit
A H
AH
van Houten
A H
AH
Hoff
A J
AJ
Lugtenburg
J
J
D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D007553:Isotope Labeling; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism*; D012242:Rhodobacter sphaeroides / Q000378:metabolism*; D014364:Tryptophan / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism*
D045322:Photosynthetic Reaction Center Complex Proteins; D014443:Tyrosine; D014364:Tryptophan