Spontaneous rhythmic activity in the intermediolateral cell nucleus of the neonate rat thoracolumbar spinal cord in vitro.
Intracellular recordings from the intermediolateral cell nucleus of the neonate rat thoracolumbar spinal cord slice preparation revealed a population of neurons which displayed three types of spontaneous rhythmic activity: burst firing, tonic beating and membrane oscillations. Most neurons displayed more than one of these types of activity. Neurons had mean resting potentials of -59 mV and input resistances ranging from 10 to 48 m omega. Spontaneous oscillations which were observed either independently or following hyperpolarization of neurons displaying tonic beating or bursting behaviour had a mean peak amplitude and frequency of approximately 14 mV and 1 Hz respectively. Oscillations were not obviously reversible as they were still apparent at potentials as negative as -120 to -140 mV. This suggests that the oscillations had a site of generation distant to the recording electrode. Neurons displaying tonic beating activity were characterized by low frequency firing activated at the peak of the depolarizing phase of the underlying oscillation and these neurons could be induced to exhibit burst behaviour by membrane depolarization. The frequency of firing in tonic beating neurons ranged from 0.1 to 8.8 Hz. Burst firing was characterized by: bursts of 3-17 action potentials; burst cycle frequency of approximately 1 Hz; an afterdepolarization potential mainly observed at the termination of a burst. Burst firing was abolished by cobalt and membrane hyperpolarization but not by barium, low calcium or tetraethylammonium chloride. The switch from tonic beating to burst firing may, in part, involve activation of a voltage- and calcium-dependent afterdepolarization potential. We conclude that a population of neurons in the lateral horn of the spinal cord are capable of rhythmic activity with underlying spontaneous pacemaker-like oscillations.
Spanswick
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Department of Physiology, University of Birmingham Medical School, U.K.
Logan
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SD
D000200:Action Potentials; D000818:Animals; D000831:Animals, Newborn; D003035:Cobalt / Q000494:pharmacology; D004558:Electric Stimulation; D004594:Electrophysiology / Q000379:methods; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000502:physiology*; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000494:pharmacology
D013757:Tetraethylammonium Compounds; D003035:Cobalt; D019789:Tetraethylammonium; C018021:cobaltous chloride