Physical map around the retinoblastoma gene: possible genomic imprinting suggested by NruI digestion.
A long-range restriction map constructed around the retinoblastoma (RB) gene by means of PFGE analysis allowed further definition of chromosomal rearrangements with a breakpoint within the gene, as well as of submicroscopic deletions. A serendipitous observation was that the NruI restriction pattern differs according to the parental origin of the rearrangement.
Blanquet
V
V
U. 173 INSERM, Hôpital Necker-Enfants-Malades, Paris, France.
Turleau
C
C
de Grouchy
J
J
Creau-Goldberg
N
N
D015139:Blotting, Southern; D002872:Chromosome Deletion; D015342:DNA Probes; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D011995:Recombination, Genetic; D015183:Restriction Mapping; D012175:Retinoblastoma / Q000235:genetics*
D015342:DNA Probes; C055654:endodeoxyribonuclease NruI; D015252:Deoxyribonucleases, Type II Site-Specific