An optimized procedure for the separation of amino acid phenylthiohydantoins by reversed-phase HPLC.
An optimization procedure for the separation of 24 PTH-amino acids by high-performance liquid chromatography on an inexpensive Merck Superspher Si 60 RP-8, (4.0 x 250 mm) column with PTH-Nle as an internal standard is described. The effects of pH, ionic strength, temperature and gradient were investigated. Using conventional HPLC equipment, the practical detection limit is about 5 pmol.
Persson
B
B
Department of Biochemistry, University of Uppsala, Sweden.
Eaker
D
D
D000596:Amino Acids / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D010669:Phenylthiohydantoin / Q000031:analogs & derivatives* / Q000302:isolation & purification; D015203:Reproducibility of Results; D013696:Temperature
D000596:Amino Acids; D010669:Phenylthiohydantoin