title: Dynamics of tracer distribution in radiation induced brain oedema in rats.
abstract: The dynamic behaviour of the distribution of Evans blue (EB), sodium fluorescein (SF), Lucifer yellow (LY) and horseradish peroxidase (HRP) was studied using standard light- and fluorescence microscopy following ultraviolet radiation induced brain oedema in the rat model. The cerebral cortex was irradiated after craniotomy (2 x 2 mm) under anaesthesia. The tracers were injected (iv) 30 min prior to radiation. Animals were perfused with glutaraldehyde through the heart at different survival periods ranging from 30 min to 24 h post irradiation. The results showed a remarkable difference in distribution and spread of these tracers in the oedematous brain following radiation. The extravasation of EB was evident in ipsilateral cortex 6 h after radiation which extended to the contralateral side at the end of the 24 h survival period. The HRP reaction product was seen in the necrotic area 3 h after radiation which further extended to the underlying white matter at 24 h survival. The LY stained the ipsilateral micronecrotic area 6 h after radiation, whereas a non-specific diffuse fluorescence of SF was noted at this time period. These results point out a specific selectivity of tracer distribution in oedematous brain following ultraviolet radiation.
authors.affiliation: Department of Neurosciences, UPV, Lejona, Spain.
authors.forename: J V
authors.initials: JV
authors.lastname: Lafuente
authors.forename: E
authors.initials: E
authors.lastname: Pouschman
authors.forename: J
authors.initials: J
authors.lastname: Cervós-Navarro
authors.forename: H S
authors.initials: HS
authors.lastname: Sharma
authors.forename: C
authors.initials: C
authors.lastname: Schreiner
authors.forename: M
authors.initials: M
authors.lastname: Korves
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier; D001929:Brain Edema / Q000209:etiology / Q000378:metabolism*; D002199:Capillary Permeability; D005070:Evans Blue; D019793:Fluorescein; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006735:Horseradish Peroxidase; D007546:Isoquinolines; D008970:Molecular Weight; D011833:Radiation Injuries, Experimental; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D007546:Isoquinolines; D005070:Evans Blue; C017475:lucifer yellow; D006735:Horseradish Peroxidase; D019793:Fluorescein