The virulent bacteriophage IRA of Salmonella typhimurium: cloning of phage genes which are potentially lethal for the host cell.
Morphological and biological properties of Salmonella typhimurium phage IRA were studied. The phage is a member of the Styloviridae family and exhibits a very wide spectrum of lytic activity. Molecular cloning of phage genes whose expression is lethal for the host cell has been performed and consequences of gene expression have been investigated. Expression of recombinant plasmid pKI71 causes structural changes of the cell wall and also degradation of host DNA while plasmid DNA remains intact. Expression of pKI72 blocks normal cell division. The possibility of applying such recombinant clones in marking pathways of microbial contamination in water areas is proposed.
Adamia
R S
RS
Institute of Vaccines and Sera, Scientific-Industrial Union, Bacteriophage, Tbilisi, Georgia, USSR.
Matitashvili
E A
EA
Kvachadze
L I
LI
Korinteli
V I
VI
Matoyan
D A
DA
Kutateladze
M I
MI
Chanishvili
T G
TG
D001433:Bacteriolysis; D002473:Cell Wall / Q000378:metabolism; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis*; D015967:Gene Expression Regulation, Viral; D006868:Hydrolysis; D008854:Microscopy, Electron; D010957:Plasmids; D015183:Restriction Mapping; D012483:Salmonella Phages / Q000235:genetics* / Q000502:physiology / Q000648:ultrastructure; D012486:Salmonella typhimurium / Q000235:genetics / Q000648:ultrastructure*
D004269:DNA, Bacterial; D004279:DNA, Viral