Human spermidine synthase gene: structure and chromosomal localization.
The human spermidine synthase (EC 2.5.1.16) gene was isolated from a genomic library constructed with DNA obtained from a human immunoglobulin G (IgG) myeloma cell line. Subsequent sequence analyses revealed that the gene comprised of 5,818 nucleotides from the cap site to the last A of the putative polyadenylation signal with 8 exons and 7 intervening sequences. The 5'-flanking region of the gene was extremely GC rich, lacking any TATA box but containing CCAAT consensus sequences. No perfect consensus sequence for the cAMP-responsive element for the AP-1 binding site was found, yet the gene contained seven AP-2 binding site consensus sequences. The putative polyadenylation signal was an unusual AATACA instead of AATAAA. Polymerase chain reaction analysis with DNA obtained from human x hamster somatic cell hybrids indicated that human spermidine synthase genomic sequences segregate with human chromosome 1. Transfection of the genomic clone into Chinese hamster ovary cells displaying a low endogenous spermidine synthase activity revealed that the gene was transiently expressed and hence in all likelihood represents a functional gene.
Myöhänen
S
S
Department of Biochemistry & Biotechnology, University of Kuopio, Finland.
Kauppinen
L
L
Wahlfors
J
J
Alhonen
L
L
Jänne
J
J
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002878:Chromosomes, Human, Pair 1; D004247:DNA; D015342:DNA Probes; D015698:Genomic Library; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D011436:Spermidine Synthase / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D004247:DNA; D011436:Spermidine Synthase