Blood clearance and organ distribution and tissue concentration of native, homopolymerized and IgG-conjugated ribosome-inactivating proteins.
1. The blood clearance, organ distribution and tissue concentration of several native, homopolymerized, and IgG-conjugated 125I-labelled ribosome-inactivating proteins (RIPs) were determined in mice. 2. Native RIPs were cleared rapidly from blood, with half-lives of 4-8 min, and were concentrated mainly in the kidneys. 3. After conjugation to IgG the three RIPs studied showed increased blood half-lives and decreased concentrations in the kidneys. 4. The two homopolymers studied had blood half-lives and kidney concentrations intermediate to those of free and conjugated RIPs. 5. These results indicate that after IgG-conjugation the increased half-lives of the RIPs studied were at least in part due to the larger molecular size of the conjugates and to their lower renal excretion.
Barbieri
L
L
Dipartimento di Patologia Sperimentale, Università di Bologna, Italy.
Battelli
M G
MG
Stirpe
F
F
D000818:Animals; D000963:Antimetabolites / Q000378:metabolism / Q000493:pharmacokinetics; D000972:Antineoplastic Agents, Phytogenic / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics; D006023:Glycoproteins / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics / Q000502:physiology; D007074:Immunoglobulin G / Q000378:metabolism; D000922:Immunotoxins / Q000378:metabolism / Q000493:pharmacokinetics* / Q000494:pharmacology; D007668:Kidney / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D051379:Mice; D009699:N-Glycosyl Hydrolases; D010940:Plant Proteins / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics; D011108:Polymers / Q000378:metabolism / Q000494:pharmacology; D011500:Protein Synthesis Inhibitors / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics*; D054789:Ribosome Inactivating Proteins, Type 1; D054790:Ribosome Inactivating Proteins, Type 2; D000076985:Saporins; D014018:Tissue Distribution; D014118:Toxins, Biological
D000963:Antimetabolites; D000972:Antineoplastic Agents, Phytogenic; D006023:Glycoproteins; D007074:Immunoglobulin G; D000922:Immunotoxins; D010940:Plant Proteins; D011108:Polymers; D011500:Protein Synthesis Inhibitors; D054789:Ribosome Inactivating Proteins, Type 1; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C051829:bryodin; C057422:trichokirin protein, Trichosanthes kirilowii; C025674:GEL protein, Gelonium multiflorum; D009699:N-Glycosyl Hydrolases; D000076985:Saporins; C024275:pokeweed antiviral protein