Occurrence of cholesterol 7 alpha- and 7 beta-hydroperoxides in rat skin as aging markers.
Evidence for presence of cholesterol 7 alpha- and 7 beta-hydroperoxides in rat skin was presented for the first time. The 7-hydroperoxides in rat skin were reduced with sodium borohydride and trimethylsilylated for identification with the authentic compounds by gas chromatography/mass spectrometry. A content of cholesterol 7-hydroperoxides in rat skin, determined by high performance liquid chromatography with a chemiluminescence detector, highly correlated with the age of rats (r = 0.874; between 1 and 45 weeks old), indicating that cholesterol 7 alpha- and 7 beta-hydroperoxides were good markers for aging.
Ozawa
N
N
Drug Metabolism & Analytical Chemistry Research, Upjohn Pharmaceuticals Limited Tsukuba Research Laboratories, Ibaraki, Japan.
Yamazaki
S
S
Chiba
K
K
Aoyama
H
H
Tomisawa
H
H
Tateishi
M
M
Watabe
T
T
D000375:Aging; D000818:Animals; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D007536:Isomerism; D008054:Lipid Peroxides / Q000032:analysis*; D008163:Luminescent Measurements; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000737:chemistry / Q000254:growth & development*
D008054:Lipid Peroxides; C041011:cholesterol 7-hydroperoxide; D002784:Cholesterol