Thin-layer chromatography and densitometry in drug assay: comparison of methods for monitoring valproic acid in plasma.
A thin-layer chromatographic (TLC) method is described for the assay of the anti-epileptic drug, valproic acid, in plasma. The use of high-performance (HPTLC) plates gave sensitive detection limits (4.87 micrograms ml-1) for derivatives of valproic acid and the reproducibility on the same or different plates was good. Comparison with high-performance liquid chromatography showed a similar performance of plate and column.
Corti
P
P
Dipartimento Farmaco Chimico Tecnologico, Università degli Studi di Siena, Italy.
Cenni
A
A
Corbini
G
G
Dreassi
E
E
Murratzu
C
C
Caricchia
A M
AM
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D003720:Densitometry / Q000379:methods; D014635:Valproic Acid / Q000097:blood*
D014635:Valproic Acid