title: Interaction of DNA with nuclear skeleton in rat hepatocytes.
abstract: We have characterized fractions liberated from rat liver cell nuclei digested with DNase I and treated with buffers containing 0.1, 0.5 and 2 M NaCl. Analysis of DNA and proteins present in these fractions as well as in nuclear matrix, confirms the chromatin model according to which transcriptionally active loops interact differently with nuclear skeleton than inactive ones.
authors.affiliation: Institute of Oncology, Department of Tumour Biology, Gliwice, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Widłak
authors.forename: J
authors.initials: J
authors.lastname: Rogoliński
authors.forename: J
authors.initials: J
authors.lastname: Rzeszowska-Wolny
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003850:Deoxyribonuclease I; D005796:Genes; D008099:Liver / Q000378:metabolism*; D015530:Nuclear Matrix / Q000378:metabolism*; D009857:Oncogenes; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004247:DNA; D003850:Deoxyribonuclease I