Modulation of splenic lymphocyte activities by a new hypoxanthine derivative (ST 789) in immunosuppressed mice.
Splenic lymphocytes of experimentally-immunosuppressed mice of different age (10 weeks and 6 months) were studied to evaluate their functional response following subcutaneous and intraperitoneal treatment with the hypoxanthine derivative N-alpha-5-(1,6-dihydro-6-oxo-9-purinyl)pentyloxy-carbonyl-L- arginine (ST 789). Experimental immunosuppression was carried out by injecting hybrid B6D2F1 mice with a single dose of cyclophosphamide (100 mg/kg, i.p.) 2 hours prior to treatment with ST 789. In the young, we found that ST 789 markedly restored the splenocyte proliferative response, assessed as total amount of 3H-thymidine incorporated by mitogen-stimulated cells. In the adult, however, the ST 789-induced recovery was less pronounced. Finally, the effects of ST 789 treatment on Con A-induced IL-2 production by splenocytes were studied in normal and immunosuppressed mice of 10 weeks.
Ruggiero
V
V
Dept. of Immunology and Microbiology, Sigma Tau, Pomezia, Italy.
Albertoni
C
C
Manganello
S
S
Foresta
P
P
Martelli
E A
EA
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003520:Cyclophosphamide / Q000494:pharmacology; D007042:Hypoxanthines / Q000494:pharmacology*; D007165:Immunosuppression Therapy; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000166:cytology*
D007042:Hypoxanthines; D007376:Interleukin-2; C054420:PCF 39; D003208:Concanavalin A; D003520:Cyclophosphamide; D001120:Arginine