Retinoic acid and alpha-difluoromethylornithine induce different expression of neural-specific cell adhesion molecules in differentiating neuroblastoma cells.
Human neuroblastoma cells SK-N-BE(2) can be induced to differentiate towards a neuronal phenotype by retinoic acid (RA) or a schwannian/glial phenotype by alpha-difluoromethylornithine (DFMO), producing differential binding of 14 antibodies (MAbs). RA induced the expression of the neural cell adhesion molecule, NCAM (also confirmed by northern blot); whereas DFMO enhanced the binding of MAbs UJ181.4, UJ127.11 which recognise an identical protein doublet of 220-240 kDa, thought to be the L1 protein(s). The data presented demonstrate that neuroblastoma cells differentiate toward separate phenotypes associated with a specific induction of two different adhesion molecules, NCAM on neuronal cells and L1 on schwannian/glial cells.
Melino
G
G
Dept. Experimental Medicine, Tor Vergata University, Rome, Italy.
Piacentini
M
M
Patel
K
K
Annicchiarico-Petruzzelli
M
M
Piredda
L
L
Kemshead
J T
JT
D000911:Antibodies, Monoclonal; D015152:Blotting, Northern; D015153:Blotting, Western; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis / Q000235:genetics*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D000518:Eflornithine / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008959:Models, Neurological; D008970:Molecular Weight; D009447:Neuroblastoma / Q000235:genetics / Q000473:pathology; D009474:Neurons / Q000166:cytology; D010641:Phenotype; D014212:Tretinoin / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D015816:Cell Adhesion Molecules, Neuronal; D014212:Tretinoin; D000518:Eflornithine