Detection of nephritis strain-associated streptokinase by monoclonal antibodies.
Monoclonal antibodies (MAbs) N-59 and RU-1 were produced by immunisation of mice with streptokinase secreted by Streptococcus group A, type 12, strain A374 isolated from a patient with post-streptococcal glomerulonephritis (PSGN) and were characterised by Western blot analysis. MAb N-59 recognised antigenic determinants shared by both nephritis strain-associated streptokinase (NSA-SKase) and streptokinase of Streptococcus group C (C-SKase); MAb RU-1 reacted only with NSA-SKase. All nephritis-associated group A streptococcal strains tested reacted with MAb N-59; 87.5% of these strains reacted with MAb RU-1. MAb N-59 reacted with SKase produced by group G streptococcal strains isolated from patients with PSGN, and MAb RU-1 recognised SKase in two out of three of these strains.
Ohkuni
H
H
Division of Immunology, Nippon Medical School, Kanagawa, Japan.
Todome
Y
Y
Yoshimura
K
K
Yamamoto
T
T
Suzuki
H
H
Yokomuro
K
K
Johnston
K H
KH
Zabriskie
J B
JB
D000818:Animals; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins / Q000302:isolation & purification*; D015153:Blotting, Western; D005921:Glomerulonephritis / Q000201:enzymology / Q000382:microbiology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D013292:Streptococcus agalactiae / Q000201:enzymology; D013297:Streptococcus pyogenes / Q000032:analysis / Q000201:enzymology*; D013300:Streptokinase / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; C042387:nephritis-strain associated protein, group A Streptococcus; D013300:Streptokinase