title: [Catalytic properties of cholinesterases immobilized in a gelatin membrane].
abstract: Catalytic properties of human blood erythrocyte acetylcholinesterase and horse blood serum butyrylcholinesterase immobilized and nonimmobilized in the gelatin membrane have been comparatively studied. Cholinesterase immobilization induces an increase in the Michaelis constant value and a decrease in the maximum rate value in reactions of enzymic hydrolysis of thiocholine ethers, but exerts no effect on these kinetic parameters in case of enzymic hydrolysis of indophenylacetate. The effect of reversible inhibitors: galanthamine, N-methyl-4-piperidinyl benzylate and 1,2,3,4-tetrahydro-9-aminoacridine (tacrine), as well as of irreversible inhibitors: O-ethyl-O-(4-nitrophenyl)ethyl phosphonate (armin), diisopropyl fluorophosphate (DFP), O,O-diethyl-O-(4-nitrophenyl) phosphate (paraoxon) and O,O-dimethyl-O-(2,2-dichlorovinyl) phosphate (DDVP) on immobilized cholinesterases is weaker as compared with the effect on nonimmobilized enzymes. The results obtained are discussed for the effect of immobilization on the catalytically active enzyme surface.
authors.forename: L P
authors.initials: LP
authors.lastname: Kuznetsova
authors.forename: L I
authors.initials: LI
authors.lastname: Kugusheva
authors.forename: E B
authors.initials: EB
authors.lastname: Nikol'skaia
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002091:Butyrylcholinesterase / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D002384:Catalysis; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005780:Gelatin; D006736:Horses; D006801:Humans; D007700:Kinetics; D008567:Membranes, Artificial; D013379:Substrate Specificity
chemical_list: D002800:Cholinesterase Inhibitors; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D005780:Gelatin; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase
vernacular_title: Kataliticheskie svoĭstva kholinésteraz, immobilizovannykh v zhelatinovoĭ membrane.