Protein kinase C and linoleic acid-induced inhibition of melanogenesis.
Linoleic acid has been shown to inhibit melanogenesis in cultured B16 mouse melanoma cells. We report here the possible involvement of protein kinase C (PKC) in linoleic acid-induced inhibition of melanogenesis in B16 cells. A single PKC subspecies (alpha-PKC) was detected in B16 cells. The enzyme was activated by linoleic acid in vitro. The effective concentrations at which PKC was activated (25 microM; maximum response) were consistent with those for the inhibition of melanogenesis in cell culture system. In addition, the permeable diacylglycerol 1-oleoyl-2-acetyl glycerol that activates PKC also inhibits melanogenesis at 100 microM. These results suggest that activation of PKC plays a pivotal role in the linoleic acid-induced inhibition of melanogenesis in B16 cells.
Ando
H
H
Department of Dermatology, Kobe University School of Medicine, Japan.
Oka
M
M
Ichihashi
M
M
Mishima
Y
Y
D000818:Animals; D004075:Diglycerides / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000502:physiology*; D008543:Melanins / Q000096:biosynthesis*; D008546:Melanoma, Experimental; D051379:Mice; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000502:physiology*; D014407:Tumor Cells, Cultured
D004075:Diglycerides; D008041:Linoleic Acids; D008543:Melanins; C038237:1-oleoyl-2-acetylglycerol; D019787:Linoleic Acid; D011493:Protein Kinase C