[Expression of class II HLA antigens on cryopreserved isolated mononuclear splenic cells].
The human spleen presents a rich source of class 2 HLA-positive mononuclear cells. After cryopreservation in liquid nitrogen and storage during 18 months these cells retain high viability and continue expressing class 2 HLA antigens on their membrane. Cryopreserved cells react specifically with antibodies to particular determinants of class 2 HLA-DR molecules. This permits their using as target-cells in serological reactions, and serves the basis for creation of banks of well-characterized "standard" class 2 HLA-positive cells with the known phenotype.
Kaluzhina
N N
NN
Abramov
V Iu
VIu
D002462:Cell Membrane / Q000276:immunology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D015925:Cryopreservation / Q000379:methods*; D006681:HLA-D Antigens / Q000032:analysis*; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000276:immunology* / Q000502:physiology; D013154:Spleen / Q000166:cytology*; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*
D006681:HLA-D Antigens
Izuchenie ékspressii HLA-antigenov II klassa na kriokonservi- rovannykh mononuklearnykh kletkakh selezenki.