Procainamide inhibits DNA methyltransferase in a human T cell line.
Procainamide, a widely used antiarrythmic, causes DNA hypomethylation in the human T cell line Jurkat, but the mechanism is unknown. We report that procainamide inhibits the DNA methyltransferase catalyzed transfer of methyl groups from S-adenosylmethionine to DNA, but has no effect on other known regulators of DNA methylation. Our results suggest that procainamide could inhibit cellular DNA methylation by inhibiting DNA methyltransferase activity.
Scheinbart
L S
LS
Department of Internal Medicine, University of Michigan, Ann Arbor 48109-0531.
Johnson
M A
MA
Gross
L A
LA
Edelstein
S R
SR
Richardson
B C
BC
D000818:Animals; D002460:Cell Line; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000378:metabolism*; D008745:Methylation; D010743:Phospholipids / Q000378:metabolism; D011342:Procainamide / Q000494:pharmacology*; D051381:Rats; D012435:S-Adenosylhomocysteine / Q000378:metabolism*; D012436:S-Adenosylmethionine / Q000378:metabolism*; D013601:T-Lymphocytes / Q000201:enzymology*
D010743:Phospholipids; D012436:S-Adenosylmethionine; D012435:S-Adenosylhomocysteine; D004248:DNA (Cytosine-5-)-Methyltransferases; D011342:Procainamide