Antineoplastic agents, 177. Isolation and structure of phyllanthostatin 6.
The isolation and structural elucidation of a new Phyllanthus glycoside, phyllanthostatin 6 [7], was summarized. Phyllanthostatin 6 [7] was isolated from the roots of Phyllanthus acuminatus (Euphorbiaceae) and was found to inhibit (ED50 = 0.35 micrograms/ml) growth of the murine P-388 lymphocytic leukemia cell line. Two other new constituents were shown to be didesacetylphyllanthostatin 3 [9] and descinnamoylphyllanthocindiol [10]. Structure determinations were achieved employing hrfabms and 2D-nmr spectroscopy. Application of an hplc separation technique to the Phyllanthus glycosides and development of a new isolation procedure for the major antineoplastic constituent, phyllanthoside [1], are also described.
Pettit
G R
GR
Cancer Research Institute, Arizona State University, Tempe 85287-1604.
Schaufelberger
D E
DE
Nieman
R A
RA
Dufresne
C
C
Saenz-Renauld
J A
JA
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification; D001572:Benzofurans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007941:Leukemia P388 / Q000188:drug therapy; D051379:Mice; D015394:Molecular Structure; D010944:Plants / Q000032:analysis; D011714:Pyrans / Q000302:isolation & purification; D012717:Sesquiterpenes; D013057:Spectrum Analysis; D013141:Spiro Compounds; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D001572:Benzofurans; D006027:Glycosides; D011714:Pyrans; D012717:Sesquiterpenes; D013141:Spiro Compounds; C068503:phyllanthostatin 6; C068504:didesacetylphyllanthostatin 3; C068502:descinnanoylphyllanthocindiol; C014754:phyllanthoside