Simultaneous liquid chromatographic determination of cholesterol, phytosterols and tocopherols in foods.
A method is described for the simultaneous determination of cholesterol, phytosterols and tocopherols in dairy and non-dairy high-performance liquid chromatography. A facile saponification and extraction scheme is completed rapidly within a single reaction tube. Clean-up is not required, with analysis completed by one of two alternative reversed-phase approaches. Sterols are monitored by short-wave ultraviolet spectrophotometry, and tocopherols by means of a series fluorescence detector. Cholesterol is resolved from the principal plant phytosterols, while the tocopherol congeners are well separated, thereby offering complementary information regarding source-lipid composition in foods under regulatory quality-control conditions. Squalene, an essential metabolic sterol precursor, can also be determined concurrently at the same viewing wavelength.
Indyk
H E
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Anchor Products Ltd., Waitoa, New Zealand.
D000818:Animals; D002784:Cholesterol / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D008892:Milk / Q000737:chemistry; D010840:Phytosterols / Q000032:analysis*; D011786:Quality Control; D014810:Vitamin E / Q000032:analysis*
D010840:Phytosterols; D014810:Vitamin E; D002784:Cholesterol