[Evaluation of a latex agglutination method for the rapid identification of Campylobacter jejuni/coli].
A latex agglutination assay was developed to identify Campylobacter jejuni and Campylobacter coli. We evaluated the specificity, reproducibility and utility of the assay for clinical use and the following results were obtained. 1) To prepare standardized antigen, bacterial cells must be suspended to a density of 1 to 5 McFarland unit, and heated at 121 degrees C for 10 to 30 min. 2) Bacterial cells may be suspended either in the solution provided with the kit, or in physiological saline, without affecting the results. 3) Of C. jejuni, 94 strains, 6 of C. coli, and 3 of "Campylobacter upsaliensis", all tested positive without exception. All other Campylobacter species, encompassing 13 species and 80 strains, were negative. An additional 9 species and 30 strains, of non-Campylobacter gram negative bacteria, isolated on the Campylobacter selection agar medium, also were uniformly negative. Based on these results, we conclude that bacteria testing positive with the kit can be identified as C. jejuni/coli. Interestingly, "C. upsaliensis", although isolated very rarely from the clinical specimens, also tested positive.
Yamazaki
E
E
Department of Clinical Laboratories, Tokyo Metropolitan Fuchu Hospital.
Koganei
M
M
Furuta
M
M
Kanome
Y
Y
Sakamoto
Y
Y
Ueshima
T
T
Mizuguchi
K
K
Takahashi
M
M
Kai
A
A
Itoh
T
T
D002167:Campylobacter / Q000145:classification; D016123:Campylobacter jejuni / Q000145:classification*; D005069:Evaluation Studies as Topic; D007841:Latex Fixation Tests / Q000379:methods* / Q000592:standards; D011933:Reagent Kits, Diagnostic / Q000592:standards
D011933:Reagent Kits, Diagnostic