A comparative study of two methods for the isolation of human leucocytes for DNA extraction.
The 'Dextran' and the 'Buffy-coat' methods for isolation of human leucocytes for DNA extraction were compared on the basis of DNA yield from the same amounts (10 ml) of blood. Human leucocytes from a total of 11 samples were isolated using both methods for each sample after which DNA was extracted. Extracted DNA samples were treated with ribonucleases and proteinase K after which the yields were quantitated by measuring absorbance at 260 nm. The 'Buffy-coat' method yielded a mean concentration of DNA of 476.7 micrograms/ml (range: 212 to 700 micrograms/ml) while the 'Dextran' method yielded 188.4 micrograms/ml (range: 64 to 340 micrograms/ml). The difference was confirmed by subjecting the extracted DNA samples to agarose gel electrophoresis.
Lim
L H
LH
Division of Haematology, Faculty of Medicine, National University of Malaysia, Kuala Lumpur.
Ton
S H
SH
Cheong
S K
SK
D001773:Blood Cells / Q000378:metabolism; D002469:Cell Separation / Q000379:methods*; D004247:DNA / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*
D004247:DNA