Stability and activity of 20 beta-hydroxysteroid dehydrogenase in microemulsion of non-ionic detergents.
We report here the formation of a microemulsion with non-ionic detergents and cyclohexane. The activity and stability of 20 beta-hydroxysteroid dehydrogenase solubilized in all water systems and in microemulsions of Nonidet P-40: Triton X-35/water/cyclohexane was investigated. In the microemulsion the activity depended on the molecular ratio of water to surfactant (Wo); maximal activity was obtained at Wo of 8.4. The stability in the microemulsion was higher at Wo = 11.75 i.e. the enzyme, retained about 50% of activity after eight days.
Ayala
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Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, D.F.
Mendoza-Hernández
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D002021:Buffers; D003349:Cortisone Reductase / Q000378:metabolism*; D003510:Cyclohexanes / Q000494:pharmacology; D003902:Detergents; D004655:Emulsions; D004795:Enzyme Stability; D007700:Kinetics; D012995:Solubility; D012996:Solutions
D002021:Buffers; D003510:Cyclohexanes; D003902:Detergents; D004655:Emulsions; D012996:Solutions; D003349:Cortisone Reductase