Rapid and standardized quantitation of hemolytic activity of the fourth component of human complement.
Based on a method that uses the fourth component of complement (C4)-deficient guinea pig serum to quantify the hemolytic activity of C4, we developed an assay that allows the processing of a large number of individual samples with high reproducibility. In contrast to the conventional procedure using titration curves of each sample to be determined, we can show that a single appropriate dilution of the sample allows accurate quantitation of hemolytic activity. The reliability of the procedure is demonstrated using either C4A- or C4B- deficient and normal individual samples.
Kaufmann
T
T
Institut für Rechstmedizin, Johannes-Gutenberg-Universität Mainz, FRG.
Kytzia
H J
HJ
Rittner
C
C
D003181:Complement C4 / Q000235:genetics / Q000276:immunology*; D003171:Complement Pathway, Classical; D004912:Erythrocytes / Q000276:immunology; D006461:Hemolysis; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D011110:Polymorphism, Genetic
D003181:Complement C4