N-acetylation of serotonin, octopamine and dopamine by adult Brugia pahangi.
The metabolism of biogenic amines by the filarial worm, Brugia pahangi, was investigated by incubating cut worms with radio-labelled amine substrates. Two-dimensional thin-layer chromatography and analysis on two high-performance liquid chromatography systems showed that [14C]5-hydroxytryptamine was metabolised to a less polar compound that was identified as N-acetyl 5-hydroxytryptamine. N-Acetyloctopamine and N-acetyldopamine were also formed when cut B. pahangi were incubated with [14C]octopamine and [3H]dopamine, respectively. N-Acetyltransferase activity towards 5-hydroxytryptamine was readily detected in nematode homogenates. This enzyme was localised in a 50,000 x g supernatant and required the addition of the co-substrate, acetyl CoA, for activity. No evidence was obtained for the involvement of monoamine oxidases in the metabolism of 5-HT in these filarial worms.
Isaac
R E
RE
Department of Pure and Applied Biology, University of Leeds, U.K.
Muimo
R
R
MacGregor
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AN
D000107:Acetylation; D000818:Animals; D002009:Brugia / Q000201:enzymology / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004298:Dopamine / Q000378:metabolism*; D009655:Octopamine / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism*
D009655:Octopamine; D012701:Serotonin; D004298:Dopamine