[Aggregates of islet cells, their structure and insulin-producing activity during cultivation in vitro].
Purified rat islets were dissociated into single-cell suspension with an EDTA-Trypsin treatment. During a stationary culture in vitro the islet cells reassociated forming aggregates (neoislets). Electron microscopy revealed that the aggregates consisted mostly of beta-cells and not numerous alpha-cells. They showed a good insulin-secreting capacity and were able to increase insulin release in response to glucose plus theophylline. The lack of passenger leukocytes makes the neoislets particularly suited for experimental and clinical transplantation.
Ablamunits
V G
VG
Bliumkin
V N
VN
Mogilevskiĭ
G M
GM
Kirsanova
L A
LA
Baranova
F S
FS
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000033:anatomy & histology / Q000378:metabolism; D016381:Islets of Langerhans Transplantation; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013806:Theophylline / Q000494:pharmacology
D003470:Culture Media; D007328:Insulin; D013806:Theophylline; D005947:Glucose
Agregaty ostrovkovykh kletok, ikh struktura i insulinprodutsiruiushchaia aktivnost' v usloviiakh kul'tivirovaniia in vitro.