title: Calcium mobilization in activated mast cells monitored by flow cytometric analysis.
abstract: Changes in intracellular calcium levels induced in mast cells by either antigen or ionomycin were monitored using flow cytometry and the calcium binding dye indo-1. Both stimuli increased calcium levels in responsive cells by a similar amount, but not all cells examined were responsive to antigen. Antigen-induced increases in intracellular calcium levels could be completely blocked by the calcium antagonist TMB-8. Flow cytometry appears to be a useful method for monitoring mast cell calcium concentrations, as it provides the capability to study large numbers of individual cells.
authors.affiliation: Department of Medicine, University of California, San Diego School of Medicine.
authors.forename: G L
authors.initials: GL
authors.lastname: Rossi
authors.forename: D J
authors.initials: DJ
authors.lastname: Young
authors.forename: S I
authors.initials: SI
authors.lastname: Wasserman
authors.forename: K E
authors.initials: KE
authors.lastname: Barrett
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002478:Cells, Cultured; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D007211:Indoles; D015759:Ionomycin / Q000494:pharmacology; D007700:Kinetics; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D051379:Mice
chemical_list: D000941:Antigens; D002121:Calcium Channel Blockers; D005456:Fluorescent Dyes; D007211:Indoles; D015759:Ionomycin; C006014:8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate; D005707:Gallic Acid; C048960:indo-1; D002118:Calcium