Separation of rat pituitary secretory granules by continuous flow electrophoresis.
The separation of growth hormone-containing cytoplasmic secretory granules from the rat pituitary gland by continuous flow electrophoresis is described. The results are consistent with the hypothesis that granule subpopulations can be separated due to differences in surface charge; these, in turn, may be related to the oligomeric state of the hormone.
Hayes
D
D
Department of Molecular and Cell Biology, Penn State University, University Park 16802.
Exton
C
C
Salada
T
T
Shellenberger
K
K
Waddle
J
J
Hymer
W C
WC
D000818:Animals; D003594:Cytoplasmic Granules / Q000648:ultrastructure*; D004586:Electrophoresis; D010902:Pituitary Gland / Q000648:ultrastructure*; D051381:Rats