Molecular cloning and sequence analysis of cDNA coding for rat liver hemoprotein H-450.
cDNA clones coding for hemoprotein H-450 were isolated from a rat liver cDNA library using anti-H-450 antibody. The molecular weight calculated from the deduced amino acid sequence comprising 547 amino acid residues was 60,085. The N-terminal sequence and a partial internal amino acid sequence of purified H-450, which were determined chemically, were both found in the amino acid sequence of H-450 deduced from the nucleotide sequence. H-450 mRNA is expressed in liver, kidney, and brain. A homology search of amino acid sequences indicated that H-450 shows no homology with cytochrome P-450, but shows significant homology with bacterial O-acetylserine (thiol)-lyases. However, H-450 has no O-acetylserine (thiol)-lyase activity.
Ishihara
S
S
Department of Biology, Faculty of Science, Kyushu University, Fukuoka.
Morohashi
K
K
Sadano
H
H
Kawabata
S
S
Gotoh
O
O
Omura
T
T
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D015698:Genomic Library; D006420:Hemeproteins / Q000096:biosynthesis / Q000235:genetics*; D008099:Liver / Q000737:chemistry*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D000906:Antibodies; D006420:Hemeproteins; D012333:RNA, Messenger; C068495:hemoprotein H-450; D004247:DNA