[Histochemical identification of endogenous lectins using labelled neoglycoproteins in human head-and-neck squamous cell carcinoma].
According to the "Population-based cancer register" of the Federal Republic of Germany only malignant neoplasms of the buccal cavity, the pharynx and larynx as well as cancers of the respiratory tract show an increasing rate of incidence and mortality. The molecular mechanisms and etiological factors causing this phenomenon are still little understood despite intensive research work. Recognition between receptors on a cellular level may be mediated by specific amino acid sequences on the level of protein-protein recognition. Additionally, the interactions between cell sugars and the corresponding protein receptor may play a decisive role in development, regeneration and organisation of cells and tissue. The high specificity of the binding of biotinylated neoglycoproteins in tissue sections enables to detect glycohistochemically binding sites for the carbohydrate ligands of the glycosylated carrier protein. The evidence of lectins in squamous cell cancer of the oral cavity, oropharynx, larynx, and hypopharynx has not been established so far. Squamous cell cancer tissue samples of twelve patients with different tumour locations were investigated by incubation of sections of paraffin-embedded samples and application of an avidin-biotin-peroxidase complex for visualisation with synthetic biotinylated neoglycoproteins. Altogether 168 stained sections were evaluated including controls. Pronounced cytoplasmatic staining was seen with the following neoglycoproteins: sialic acid-bovine serum albumin (BSA), glucuronic acid-BSA, N-acetylglucosamine (glcNAc)-BSA, N-acetylgalactosamine (beta-galNAc)-BSA, lactose-BSA, maltose-BSA, mannose-BSA, mannose-6-phosphate-BSA. No corresponding lectins seems to exist for the following investigated sugars: fucoidan, heparin, and the alpha-anomeric form of N-acetylgalactosamine, because no specific staining was seen.(ABSTRACT TRUNCATED AT 250 WORDS)
Steuer
M K
MK
Klinik und Poliklinik für Hals-Nasen-Ohrenheilkunde, Universität Köln.
Gabius
H J
HJ
Bardosi
A
A
Matthias
R
R
D001710:Biotin; D002294:Carcinoma, Squamous Cell / Q000737:chemistry*; D006023:Glycoproteins; D006031:Glycosylation; D006258:Head and Neck Neoplasms / Q000737:chemistry*; D006801:Humans; D037102:Lectins / Q000032:analysis*; D010544:Peroxidases; D011975:Receptors, Mitogen / Q000032:analysis; D012710:Serum Albumin, Bovine
D006023:Glycoproteins; D037102:Lectins; D011975:Receptors, Mitogen; D012710:Serum Albumin, Bovine; D001710:Biotin; D010544:Peroxidases
Histochemische Identifizierung endogener Lektine durch markierte Neoglykoproteine bei humanen Kopf-Hals-Plattenepithelkarzinomen.