Immunological characterization of L-triiodothyronine and L-thyroxine labelled with rhodamine B isothiocyanate.
In the present work the label of L-T3 and L-T4 with RBITC have been developed by means of a relatively easy procedure. A purification by TLC, previously optimized, offers the possibility of obtaining a good recovery of conjugates T3 and T4-RBITC. The immunoreactivity of conjugates was calculated by a formulas especially designed for this purpose. This formulas could be applied to any molecule labelled with fluorescent compounds.
Reyes
A
A
Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Málaga, Spain.
Diéguez
J L
JL
Ruiz
M
M
Alonso
A
A
Morell
M
M
D002855:Chromatography, Thin Layer; D005456:Fluorescent Dyes; D011863:Radioimmunoassay; D012235:Rhodamines; D013050:Spectrometry, Fluorescence; D013974:Thyroxine / Q000032:analysis* / Q000276:immunology; D014284:Triiodothyronine / Q000032:analysis* / Q000276:immunology
D005456:Fluorescent Dyes; D012235:Rhodamines; D014284:Triiodothyronine; C027755:rhodamine isothiocyanate; D013974:Thyroxine