Serum levels and catabolism of 3'-azido-3'-deoxythymidine in vivo measured using a specific radioimmunoassay.
The thymidine analogue 3'-azido-3'-deoxythymidine is an effective inhibitor of HIV replication in vitro and is used in the treatment of acquired immunodeficiency syndrome. We report here upon a rapid sensitive radioimmunoassay for the detection of azidothymidine in serum or plasma. The assay is simple to perform and levels as low as 1 ng azidothymidine/ml can be detected. The assay is specific for azidothymidine and shows almost no cross-reaction with closely related nucleoside analogues or with naturally occurring nucleosides. Using this radioimmunoassay we were able to measure the azidothymidine levels in the serum of monkeys and acquired immunodeficiency syndrome patients treated with AZT. Individual variation in the peak serum level and clearance rate of azidothymidine were seen, which emphasizes the need to tailor the dose to the individual.
Cox
S
S
Department of Virology, National Bacteriological Laboratory, Stockholm, Sweden.
Rudén
U
U
Nagy
K
K
Albert
J
J
Holmberg
V
V
Sandström
E
E
Wahren
B
B
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism; D000818:Animals; D001711:Biotransformation; D003429:Cross Reactions; D006801:Humans; D007700:Kinetics; D008252:Macaca fascicularis; D011863:Radioimmunoassay / Q000379:methods; D015215:Zidovudine / Q000097:blood / Q000378:metabolism* / Q000627:therapeutic use
D015215:Zidovudine