title: Monoclonal antibodies recognizing both soluble and membrane bound catechol-O-methyltransferase.
abstract: Both cytosolic, soluble and membrane-bound catechol-O-methyl-transferase (COMT) from pig and rat liver or kidney were recognized by mouse monoclonal antibodies (MAbs) raised against soluble COMT isolated from pig liver. In ELISA, the MAbs Co 16 and Co 54 reacted better with the pig than with the rat enzyme. The MAb Co 60 showed good reactivity with both pig and rat COMT. In addition, all three MAbs recognize the soluble (23 kDa) as well as the membrane-bound (26 kDa) forms of the COMT enzyme.
authors.affiliation: Pharmacological Department, University of Florence, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Bertocci
authors.forename: G
authors.initials: G
authors.lastname: Garotta
authors.forename: G
authors.initials: G
authors.lastname: Zürcher
authors.forename: V
authors.initials: V
authors.lastname: Miggiano
authors.forename: M
authors.initials: M
authors.lastname: Da Prada
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002394:Catechol O-Methyltransferase / Q000276:immunology* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D008099:Liver / Q000201:enzymology; D008566:Membranes / Q000201:enzymology; D051379:Mice; D010624:Phenelzine; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology / Q000276:immunology; D013552:Swine
chemical_list: D000911:Antibodies, Monoclonal; D002394:Catechol O-Methyltransferase; D010624:Phenelzine