[Comparison of the biological activity and stability of menadione and menadiol in male chickens].
A bioassay of vitamin K is described, based on the prothrombin clotting time of 3-week-old, vitamin-K-depleted, and cumatetralyl-sensitized male broiler chicks, using a homologous thrombokinase preparation. With this test it could be shown that the diacetate and dibutyrate esters of menadiol are vitamin-K-active. The bioactivity of menadione from these menadiolesters amounted to about 70% of the standard menadione from a coated menadione sodium bisulfite (Dohyfral). Menadiol seems to be temperature-resistant under such conditions, whereby two uncoated MSB preparations lost about 60% of their activity.
Gropp
J
J
Institut für Physiologie, Physiologische Chemie und Ernährungsphysiologie, Ludwig-Maximilians-Universität München.
Mehringer
W
W
D000818:Animals; D001681:Biological Assay; D001682:Biological Availability; D002645:Chickens / Q000097:blood / Q000378:metabolism*; D004285:Dogs; D004355:Drug Stability; D010314:Partial Thromboplastin Time; D014812:Vitamin K / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000493:pharmacokinetics
D014812:Vitamin K; C023885:menadiol
Vergleich der biologischen Aktivität und Stabilität von Menadion und Menadiol bei Hühnerküken.