Neuropeptide Y and peptide YY stimulate the growth of exocrine pancreatic carcinoma cells.
Neuropeptides exert inhibitory effects on pancreatic secretion, but their role in the regulation of growth is unknown. This study was executed to evaluate the effects of PYY and NPY on cell growth and 3H-thymidine incorporation in human (MiaPaCa-2, Capan-2) and hamster (H2T) exocrine pancreatic carcinoma cells in vitro. A significant increase in the number of cells after 96 h of treatment with NPY was observed at 0.01 microM in H2T, 0.1 microM in MiaPCa-2 and at 1 microM in Capan-2 cells. PYY was less potent and did not increase significantly cell growth in MiaPaCa-2, but did at 0.1 microM in Capan-2 and at 1 microM concentration in H2T. Stimulation for 48h with NPY increased 3H-thymidine incorporation significantly at 0.01 microM in all cell lines. With PYY, stimulation of 3H-thymidine incorporation occurred in H2T cells at 0.01 microM. 3H-thymidine incorporation after PYY treatment was significantly increased at 0.1 microM in MiaPaCa-2 and at 1 microM in Capan-2 cells. Receptor studies showed low but definite specific binding of both NPY and PYY in all cell lines. The results suggest that NPY and PYY may have a role in the regulation of growth of exocrine pancreatic carcinoma cells.
Rämö
O J
OJ
Department of Neurosurgery, University of Cincinnati, College of Medicine, Cincinnati, OH 45267.
Balasubramaniam
A
A
Sheriff
S
S
Rogers
D H
DH
McCullough
P J
PJ
Bell
R H
RH
D000230:Adenocarcinoma / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006801:Humans; D009478:Neuropeptide Y / Q000378:metabolism / Q000494:pharmacology*; D010190:Pancreatic Neoplasms / Q000473:pathology*; D019894:Peptide YY; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology*; D013268:Stimulation, Chemical; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
D004273:DNA, Neoplasm; D009478:Neuropeptide Y; D010455:Peptides; D019894:Peptide YY