title: Lumbar subarachnoid catheterization in rats.
abstract: An animal model was developed for the study of subarachnoid (spinal) anesthesia and analgesia under unanesthetized, unsedated and unrestrained conditions. Sprague-Dawley rats were anesthetized with intraperitoneal ketamine (75-100 mg.kg-1). A PE10 catheter was inserted under direct vision into the lumbar subarachnoid space, through partial laminectomy of L1 or L2 with or without removal of adjacent intervertebral ligament. One week after surgery, correct position of the catheter was verified by subarachnoid injection of 0.03-0.05 ml of 1.5% lidocaine, which produced temporary hind limb paralysis in all but one animal in 28 consecutive operations. There was neither mortality nor major complication, intraoperatively or postoperatively. Only 2 animals developed minor subcutaneous would infections which responded to incision, drainage and debridement.
authors.affiliation: Department of Anesthesiology, New York University, School of Medicine, NY 10016.
authors.forename: B C
authors.initials: BC
authors.lastname: Wang
authors.forename: D E
authors.initials: DE
authors.lastname: Hillman
authors.forename: D
authors.initials: D
authors.lastname: Li
authors.forename: H
authors.initials: H
authors.lastname: Turndorf
mesh_terms: D000818:Animals; D002404:Catheterization / Q000662:veterinary*; D008297:Male; D011182:Postoperative Care; D051381:Rats; D011919:Rats, Inbred Strains; D013129:Spinal Puncture / Q000662:veterinary*; D013346:Subarachnoid Space