title: Isolation of a plasmid from "canine" Staphylococcus epidermidis mediating constitutive resistance to macrolides and lincosamides.
abstract: A small plasmid of 2.5 kB mediating constitutive resistance to macrolide-lincosamide-(ML)antibiotics could be detected in a "canine" Staphylococcus epidermidis-culture. This plasmid, designated as pSES 1, was identified by interspecies protoplast transformation into Staphylococcus aureus RN 4220. A detailed restriction map of pSES 1 could be constructed using the restriction endonucleases Acc I, Bcl I, Cfo I, Cla I, Hind III, Hinf I, Mbo I, Sst I and Taq I. This map allowed structural comparisons of pSES 1 with plasmids from "human" Staphylococcus- and Bacillus-species, also mediating macrolide-lincosamide resistance (MLR). On the basis of its restriction map, pSES 1 proved to be similar to the plasmids pNE 131 from "human" S. epidermidis, pE 194 from "human" S. aureus and pIM 13 from B. subtilis.
authors.affiliation: Institut für Bakteriologie und Immunologie, Justus Liebig-Universität Giessen, F.R.G.
authors.forename: S
authors.initials: S
authors.lastname: Schwarz
authors.forename: H
authors.initials: H
authors.lastname: Blobel
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D004283:Dog Diseases / Q000382:microbiology; D004285:Dogs; D004352:Drug Resistance, Microbial / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D055231:Lincosamides; D018942:Macrolides; D011523:Protoplasts; D011815:R Factors; D015183:Restriction Mapping; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000235:genetics*; D014169:Transformation, Bacterial; D014552:Urinary Tract Infections / Q000382:microbiology / Q000662:veterinary*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D055231:Lincosamides; D018942:Macrolides