title: Multimeric analysis of von Willebrand factor by molecular sieving electrophoresis in sodium dodecyl sulphate agarose gel.
abstract: We report the development and optimisation of an agarose gel electrophoretic method for the separation and detection of von Willebrand Factor (vWF) multimers. The method has been specifically developed for use in the clinical evaluation and classification of patients with von Willebrand's Disease (vWD) and clearly shows structural multimer abnormalities associated with the bleeding diathesis of this inherited bleeding disorder.
authors.affiliation: Department of Biochemistry, Alfred Hospital, Prahran, Melbourne, Victoria, Australia.
authors.forename: G
authors.initials: G
authors.lastname: Raines
authors.forename: H
authors.initials: H
authors.lastname: Aumann
authors.forename: S
authors.initials: S
authors.lastname: Sykes
authors.forename: A
authors.initials: A
authors.lastname: Street
mesh_terms: D001704:Biopolymers; D002021:Buffers; D004587:Electrophoresis, Agar Gel; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D008567:Membranes, Artificial; D011145:Polyvinyls; D014841:von Willebrand Factor / Q000737:chemistry*; D005465:Fluorocarbon Polymers
chemical_list: D001704:Biopolymers; D002021:Buffers; D008567:Membranes, Artificial; D011145:Polyvinyls; D014841:von Willebrand Factor; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers