title: An early marker of hyperoxic lung injury in the rat and its pharmacological modulation.
abstract: Of three possible early biochemical changes which were investigated in rats after hyperoxia, one was shown to be a useful marker of damage in this species. Mitochondrial oxygen uptake measured in lung homogenates has already been reported to be impaired after 24 h. With a purified mitochondrial fraction, we found significant impairment after only 3 h exposure to 100% oxygen. To our knowledge, this is the earliest significant change reported in this species. The antioxidants N-acetyl cysteine, dimethyl sulphoxide and allopurinol were found to ameliorate the injury. This suggests a possible link with the pulmonary damage and survival of rats in hyperoxia, which may be modulated also by antioxidant therapy.
authors.affiliation: Division of Anaesthesia, Clinical Research Centre, Harrow, Middlesex.
authors.forename: M J
authors.initials: MJ
authors.lastname: O'Connell
authors.forename: S D
authors.initials: SD
authors.lastname: Snape
authors.forename: J F
authors.initials: JF
authors.lastname: Nunn
mesh_terms: D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D003553:Cystine / Q000031:analogs & derivatives / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005260:Female; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
chemical_list: D000975:Antioxidants; D003553:Cystine; D000493:Allopurinol; D010100:Oxygen; D004121:Dimethyl Sulfoxide; C030905:N-monoacetylcystine