Presence of influenza virus-reactive glycophorins other than glycophorin A in human erythrocyte membranes.
The reactivities against seven hemagglutinins including influenza A and B viruses of six sialoglycoprotein (glycophorin) fractions, M-Frs.1-3 and N-Frs.1-3, separated from human OMs and ONs erythrocyte membranes by a combination of the LIS-phenol method and gel filtration were studied. The serological results show that M-Fr.1 and N-Fr.1 were glycophorins AM and AN, respectively, the reactivities against influenza A and B viruses of both M-Fr.2 and N-Fr.2 which contained glycophorins B and C were remarkably higher than that of glycophorin A and the reactivities against influenza viruses of both M-Fr.3 and N-Fr.3 which contained glycophorins B and D were considerably lower than that of glycophorin A.
Ohyama
K
K
Department of Biochemistry, Tokyo College of Pharmacy, Japan.
Yamauchi
S
S
Endo
T
T
Ohkuma
S
S
D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000276:immunology*; D006021:Glycophorins / Q000302:isolation & purification / Q000502:physiology*; D006385:Hemagglutination Inhibition Tests; D006389:Hemagglutinins, Viral / Q000276:immunology*; D006801:Humans; D009975:Orthomyxoviridae / Q000276:immunology*
D000596:Amino Acids; D002241:Carbohydrates; D006021:Glycophorins; D006389:Hemagglutinins, Viral