Production of interleukin-6 from macrophages by MDP-Lys (L 18), romurtide.
N2-[(N-Acetylmuramoyl)-L-alanyl-D-isoglutaminyl-N6-stearoyl-L-lysine [MDP-Lys(L18), romurtide] is an immunopotentiating substance. In addition to neutrophilic leukocytosis, the effects previously found after the administration of this compound in both mice and humans were thrombocytosis and elevated levels of colony-stimulating factor (CSF) in peripheral blood. Although the exact mechanism of thrombopoiesis is not yet known, evidence has been accumulating that interleukin-6 (IL-6) plays an important role in the maturation of megakaryocytes, and the administration of IL-6 has been reported to induce a significant increase in blood platelets associated with promotion of megakaryocyte maturation. We measured the IL-6 levels in the culture supernatants of peripheral blood mononuclear cells (PBMCs), adherent cells and nonadherent cells in the presence of romurtide. Significant augmentation of IL-6 from PBMCs and adherent cells, but not nonadherent cells, was observed in the presence of romurtide in vitro.
Shimoda
K
K
Cancer Center, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Okamura
S
S
Harada
N
N
Omori
F
F
Niho
Y
Y
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002478:Cells, Cultured; D004357:Drug Synergism; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000737:chemistry; D008264:Macrophages / Q000378:metabolism*
D015850:Interleukin-6; D000119:Acetylmuramyl-Alanyl-Isoglutamine; C036866:romurtide