title: Preparative scale separation of neutral lipids and phospholipids by centrifugally accelerated thin-layer chromatography.
abstract: Mixtures of lipids and phospholipids were separated by centrifugally accelerated thin-layer chromatography on a preparative scale (300-500 mg lipid mixture per run). The isolated lipids and phospholipids were identified by 1H and 13C NMR spectroscopy and their fatty acid composition was determined by GLC and GLC-MS of their methyl esters.
authors.affiliation: Institute of Pharmacy, University of Oslo, Norway.
authors.forename: S
authors.initials: S
authors.lastname: Bergheim
authors.forename: K E
authors.initials: KE
authors.lastname: Malterud
authors.forename: T
authors.initials: T
authors.lastname: Anthonsen
mesh_terms: D002498:Centrifugation; D002855:Chromatography, Thin Layer / Q000379:methods*; D005227:Fatty Acids / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D008055:Lipids / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D010743:Phospholipids / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D005227:Fatty Acids; D008055:Lipids; D010743:Phospholipids