Microbial metabolism of quinoline and related compounds. VII. Quinoline oxidoreductase from Pseudomonas putida: a molybdenum-containing enzyme.
The quinoline oxidoreductase from Pseudomonas putida was purified 50-fold to homogeneity with 21% recovery, using ammonium sulfate precipitation, hydrophobic interaction-, anion exchange-, and gel chromatography. The Mr of the native enzyme was calculated to be 300,000 by gel filtration. SDS-polyacrylamide gel electrophoresis of the enzyme revealed three protein bands corresponding to Mr 85,000, 30,000 and 20,000. The enzyme contained 8 atoms of iron, 8 atoms of acid-labile sulfide, 2 molecules of FAD, and the molybdenum cofactor, molybdopterin. Besides quinoline, the quinoline oxidoreductase also catalysed the conversion of 5-, 6-, 7- and 8-hydroxyquinoline and 8-chloroquinoline to the corresponding 2-oxo compounds. The incorporated oxygen atom was derived from water. Cyanide and methanol were effective inhibitors.
Bauder
R
R
Institut für Mikrobiologie, Universität Hohenheim.
Tshisuaka
B
B
Lingens
F
F
D003067:Coenzymes / Q000032:analysis; D005182:Flavin-Adenine Dinucleotide / Q000032:analysis; D005420:Flavoproteins / Q000032:analysis; D007501:Iron / Q000032:analysis; D008667:Metalloproteins / Q000032:analysis; D008970:Molecular Weight; D008982:Molybdenum / Q000032:analysis*; D000090006:Molybdenum Cofactors; D010088:Oxidoreductases / Q000302:isolation & purification*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D011549:Pseudomonas / Q000201:enzymology*; D011621:Pteridines / Q000032:analysis; D012988:Soil Microbiology; D013053:Spectrophotometry; D013455:Sulfur / Q000032:analysis
D003067:Coenzymes; D005182:Flavin-Adenine Dinucleotide; D005420:Flavoproteins; D007501:Iron; D008667:Metalloproteins; D008982:Molybdenum; D000090006:Molybdenum Cofactors; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; D011621:Pteridines; D013455:Sulfur; C022261:molybdopterin; C068667:quinoline 2-oxidoreductase