Acetylcholine-activated currents in quail myotubes expressing viral oncogenes.
Acetylcholine-activated currents were recorded in cultured myotubes arising from embryonic quail myoblasts transformed by the v-src and v-ras oncogenes. In src-myotubes, the whole cell inward current decayed more slowly than in non-transformed controls. In ras-myotubes, the current had a faster decay and smaller amplitude than in the controls. The single-channel conductance and mean open times recorded from cell-attached patches were similar in transformed and control cells. However, in ras-myotubes the frequency of channel openings strongly decreased with time. It is concluded that oncogenic tyrosine-specific protein kinase (v-src product) and G-like p21 protein (v-ras product) can induce differential changes in the function of nicotinic ACh receptor, perhaps related to specific biochemical events elicited in the establishment of the transformed state.
Grassi
F
F
Dipartimento di Medicina Sperimentale, Università dell'Aquila, Italy.
Gauzzi
M C
MC
Eusebi
F
F
Alemà
S
S
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D015870:Gene Expression; D011905:Genes, ras / Q000502:physiology*; D016391:Genes, src / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects / Q000235:genetics; D009132:Muscles / Q000187:drug effects / Q000502:physiology*; D011784:Quail; D014170:Transformation, Genetic
D000109:Acetylcholine