Age related changes in the turnover of proteoglycans from explants of bovine articular cartilage.
Articular cartilage in explant culture synthesized 2 proteoglycan types of different size. The proportion of 35S-sulfate incorporated into the small proteoglycan was higher in mature (17%) than in immature cartilage (11%). The chondroitin sulfate chains of both proteoglycans, synthesized by mature cartilage were shorter than those of immature cartilage, with a higher ratio of 6-sulfated over 4-sulfated disaccharides. Radiolabelled macromolecules from tissue of both ages were released in 2 phases: an initial fast release followed by a period of slow release. The half-lives of both proteoglycan populations were shorter in mature cartilage than in immature tissue (-45%). The amount of proteoglycans released during the initial short phase increases with age, from 23-24% in immature cartilage to 28-29% in mature tissue.
Brand
H S
HS
Jan van Breemen Institut, Center for Rheumatology and Rehabilitation, Amsterdam, The Netherlands.
de Koning
M H
MH
van Kampen
G P
GP
van der Korst
J K
JK
D000375:Aging / Q000378:metabolism*; D000818:Animals; D002358:Cartilage, Articular / Q000378:metabolism*; D002417:Cattle; D003470:Culture Media; D046508:Culture Techniques; D005109:Extracellular Matrix / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism; D014020:Tissue Extracts / Q000378:metabolism
D003470:Culture Media; D006025:Glycosaminoglycans; D011509:Proteoglycans; D013431:Sulfates; D014020:Tissue Extracts