[The hepatocyte of the fetal rat as an in vitro model system for testing the antioxidant activity of biological fluids].
In case of hepatocytes of the fetal rat at the end of the gestation (H.) the lipid-peroxidation was determined by measuring the malondialdehyde (MDA) and the vitality by means of the trypan-blue test. The H. with an incubation up to 120 minutes in PBS formed a high spontaneous value of MDA with a simultaneous loss of the cell activity (r = -0.89; p less than 0.05; n = 25). On the conditions of an experimentally generated activation of O2-radical-forming processes (addition of Fe2+/vit. C) concentration-depending increasing of the MDA-formation and trypan-blue reception of the H. were induced (p less than 0.01). The addition of vit. C reduced the MDA-formation and the vitality of the H. The results exclude a direct relation of the two parameters. The MDA-formation of the H. in vitro can be used for testing antioxidative effects of biological fluids.
Otting
U
U
Klinik und Poliklinik für Kinderheilkunde, Bereiches Medizin (Charité), Humboldt-Universität zu Berlin.
Hellmann
C
C
Räder
R
R
D000818:Animals; D001826:Body Fluids / Q000378:metabolism*; D002470:Cell Survival; D002478:Cells, Cultured; D015227:Lipid Peroxidation; D008099:Liver / Q000166:cytology* / Q000196:embryology; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D014343:Trypan Blue
D014343:Trypan Blue
Der Hepatozyt der fetalen Ratte als in vitro-Modellsystem zur Prüfung antioxidativer Aktivität von biologischen Flüssigkeiten.