Differential effects of methylmercuric chloride and mercuric chloride on oxidation and iodination reactions catalyzed by thyroid peroxidase.
Thyroid peroxidase (TPO), the major enzyme in the thyroid hormone synthesis, multifunctionally catalyzes (1) iodide oxidation, (2) iodination of the precursor protein, and (3) a coupling reaction of iodotyrosyl residues. The present study was carried out to examine the mercurial effects on the iodination, the second step of TPO. Purified porcine thyroglobulin or bovine serum albumin as acceptor protein was iodinated with [125I]NaI and H2O2 by purified porcine TPO. Iodinated protein was separated by acid precipitation on membrane filter or paper chromatography. Both CH3HgCl and HgCl2 dose-dependently inhibited the iodination, but HgCl2 was more potent to inhibit the iodination than CH3HgCl. These mercurial effects on the second step resemble the effects on the third step which were already reported; but are in marked contrast to the effects on the first step, where TPO was inhibited by HgCl2 but never by CH3HgCl.
Nishida
M
M
Faculty of Pharmaceutical Sciences, University of Tokushima, Shomachi, Japan.
Sato
K
K
Kawada
J
J
D000818:Animals; D006861:Hydrogen Peroxide / Q000378:metabolism; D007453:Iodide Peroxidase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D007455:Iodine / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008627:Mercuric Chloride / Q000494:pharmacology*; D008767:Methylmercury Compounds / Q000494:pharmacology*; D010084:Oxidation-Reduction; D012709:Serum Albumin / Q000378:metabolism; D012974:Sodium Iodide / Q000378:metabolism; D013552:Swine; D013954:Thyroglobulin / Q000378:metabolism
D007457:Iodine Radioisotopes; D008767:Methylmercury Compounds; D012709:Serum Albumin; D008627:Mercuric Chloride; D013954:Thyroglobulin; D007455:Iodine; D006861:Hydrogen Peroxide; D007453:Iodide Peroxidase; D012974:Sodium Iodide; C004925:methylmercuric chloride