Selective determination of altertoxins by high-performance liquid chromatography with electrochemical detection with dual "in-series" electrodes.
A selective method for the determination of altertoxin-I and altertoxin-II by high-performance liquid chromatography with electrochemical detection is described. Altertoxins were separated on a reversed-phase column with methanol-water containing 0.1 M sodium nitrate and 1 mM nitric acid (60:40) as eluent and detected with dual in-series electrodes operating in the "redox" mode (generator electrode +1.0 V, indicator electrode -0.1 V). The method was applied successfully to the determination of sub-ppm levels of altertoxins in samples of maize, rice and tomatoes infected by Alternaria alternata.
Visconti
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Istituto Tossine e Micotossine da Parassiti Vegetali, Consiglio Nazionale delle Ricerche, Via G. Amendola, Bari, Italy.
Sibilia
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Palmisano
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D001551:Benz(a)Anthracenes / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D009183:Mycotoxins / Q000032:analysis* / Q000378:metabolism; D012275:Oryza / Q000032:analysis; D010084:Oxidation-Reduction; D010569:Perylene / Q000031:analogs & derivatives; D014675:Vegetables / Q000032:analysis; D003313:Zea mays / Q000032:analysis
D001551:Benz(a)Anthracenes; D009183:Mycotoxins; C050234:altertoxin II; C050233:altertoxin I; D010569:Perylene