title: Detection of Mycoplasma hyopneumoniae DNA by the polymerase chain reaction.
abstract: DNA amplification by the polymerase chain reaction (PCR) was examined to detect DNA of Mycoplasma hyopneumoniae, an etiological agent of porcine pneumonia. A pair of synthetic primers was selected that specify the amplification of a 520-basepair DNA fragment in a reiterative sequence of M. hyopneumoniae genome. The PCR product was detected by direct gel electrophoresis or by blot hybridization to a synthetic oligonucleotide probe. The specificity of PCR for M. hyopneumoniae was confirmed by lack of cross-reactivity to DNA from other porcine mycoplasmas.
authors.affiliation: Animal Center for Biomedical Research, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Harasawa
authors.forename: K
authors.initials: K
authors.lastname: Koshimizu
authors.forename: O
authors.initials: O
authors.lastname: Takeda
authors.forename: T
authors.initials: T
authors.lastname: Uemori
authors.forename: K
authors.initials: K
authors.lastname: Asada
authors.forename: I
authors.initials: I
authors.lastname: Kato
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial; D015345:Oligonucleotide Probes