The separation and detection of alkaline oligoclonal IgG bands in cerebrospinal fluid using immobilised pH gradients.
A method is described for the separation and detection of highly alkaline IgG bands in unconcentrated cerebrospinal fluid (CSF). These bands are frequently found in the cerebrospinal fluid of patients with inflammatory diseases of the central nervous system, particularly in the case of multiple sclerosis, and their detection is an important aid in clinical diagnosis. An isoelectric focusing technique using an immobilised pH gradient in polyacrylamide gel has been developed over the pH range 7-10, producing a linear and stable pH gradient with excellent resolution. After electrofocusing, the protein patterns were blotted onto polyvinylidene difluoride membranes and visualised using anti-human IgG followed by an enzyme-labelled second antibody. Blotting could be carried out by capillary diffusion for up to 16 h duration without any loss in resolution. Using this method, highly alkaline intrathecal IgG bands were found in the cerebrospinal fluid of all of the 14 multiple sclerosis patients. There were also 2 patients with alkaline IgG bands in their cerebrospinal fluid who were not diagnosed as multiple sclerosis. By contrast, no alkaline IgG bands with an isoelectric point (pI) greater than 8.6 were found in any of the serum samples studied (n = 50) from patients with various neurological disorders including multiple sclerosis.
Cowdrey
G
G
Department of Biochemistry, Hurstwood Park Neurological Centre, Haywards Heath, Sussex, UK.
Gould
B
B
Rees
J
J
Firth
G
G
D002021:Buffers; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid*; D007136:Immunoglobulins / Q000134:cerebrospinal fluid*; D007525:Isoelectric Focusing / Q000379:methods*; D008567:Membranes, Artificial; D009103:Multiple Sclerosis / Q000175:diagnosis; D043025:Oligoclonal Bands; D011145:Polyvinyls; D005465:Fluorocarbon Polymers
D002021:Buffers; D007074:Immunoglobulin G; D007136:Immunoglobulins; D008567:Membranes, Artificial; D043025:Oligoclonal Bands; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers