The insulin signal initiating cellular differentiation is preserved by chick embryo myoblasts incubated at 2 degrees C.
Insulin pulse treatment, lasting for 1 to 3 min, stimulates differentiation of chick embryo myoblasts to myotubes and myofibers in a serum-free medium. The use of serum-free medium supplemented with 2,2'-thiodiethanol permits terminal differentiation of chick embryo myoblasts to cross-striated, spontaneously contracting myofibers. The experiments carried out showed that incubation of chick embryo myoblasts after the insulin pulse treatment for 10 days at 2 degrees C does not inhibit the progress of their differentiation. The data demonstrate that differentiation can be interrupted at any time by transferring cells to 2 degrees C and resumed without delay after returning to 37 degrees C.
Reiss
K
K
Department of Cell Biology, Jan Zurzycki Institute of Molecular Biology, Jagiellonian University, Kraków/Poland.
Kajstura
J
J
Korohoda
W
W
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D003402:Creatine Kinase / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D009132:Muscles / Q000166:cytology* / Q000196:embryology / Q000201:enzymology; D015398:Signal Transduction; D013696:Temperature
D007328:Insulin; D003402:Creatine Kinase