title: Identification by n.m.r. spectroscopy of oligosaccharides obtained by treatment of the hairy regions of apple pectin with rhamnogalacturonase.
abstract: 2D-N.m.r. methods have been used to determine the composition of a mixture of oligosaccharides obtained by enzymic degradation of the modified hairy (ramified) regions of apple pectin with a new rhamnogalacturonase. The structures of the oligosaccharides were based on the unit alpha-Rhap-(1----4)-alpha-GalA-(1----2)-alpha-Rhap-(1----4)- GalA. A-beta-Galp unit was 4-linked to approximately half of the terminal Rhap residues and to half of the (1----2)-linked Rhap residues. The sample contained a mixture of a tetrasaccharide, two pentasaccharides, and one hexasaccharide.
authors.affiliation: AFRC Institute of Food Research, Norwich, Great Britain.
authors.forename: I J
authors.initials: IJ
authors.lastname: Colquhoun
authors.forename: G A
authors.initials: GA
authors.lastname: de Ruiter
authors.forename: H A
authors.initials: HA
authors.lastname: Schols
authors.forename: A G
authors.initials: AG
authors.lastname: Voragen
mesh_terms: D002240:Carbohydrate Sequence; D005638:Fruit; D006026:Glycoside Hydrolases; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D010368:Pectins / Q000737:chemistry*
chemical_list: D009844:Oligosaccharides; D010368:Pectins; D006026:Glycoside Hydrolases; C067959:rhamnogalacturonase A