Interaction of DNA with nuclear skeleton in rat hepatocytes.
We have characterized fractions liberated from rat liver cell nuclei digested with DNase I and treated with buffers containing 0.1, 0.5 and 2 M NaCl. Analysis of DNA and proteins present in these fractions as well as in nuclear matrix, confirms the chromatin model according to which transcriptionally active loops interact differently with nuclear skeleton than inactive ones.
Widłak
P
P
Institute of Oncology, Department of Tumour Biology, Gliwice, Poland.
Rogoliński
J
J
Rzeszowska-Wolny
J
J
D000818:Animals; D004247:DNA / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003850:Deoxyribonuclease I; D005796:Genes; D008099:Liver / Q000378:metabolism*; D015530:Nuclear Matrix / Q000378:metabolism*; D009857:Oncogenes; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid
D004247:DNA; D003850:Deoxyribonuclease I