title: Construction of NotI restriction map of the Streptococcus mutans genome.
abstract: Streptococcus mutans and Streptococcus sobrinus are the major causative organisms of human dental caries. Pulsed-field gel electrophoresis (PFG) showed that the restriction enzyme NotI produced ten and six DNA fragments from the genomes of S. mutans strain MT8148 and S. sobrinus strain 6715, respectively. The sizes of the chromosomes of S. mutans and S. sobrinus were each estimated to be about 2200 kb. The NotI restriction map of S. mutans MT8148 genome was constructed by Southern blot analysis with probes that overlapped two adjacent NotI fragments. Several virulence-associated genes of S. mutans were placed on the NotI restriction map. In addition, unique 'fingerprints' of S. mutans chromosomal DNA digested with NotI were produced by PFG, and these may be useful for epidemiological studies.
authors.affiliation: Department of Dental Research, National Institute of Health, Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Okahashi
authors.forename: C
authors.initials: C
authors.lastname: Sasakawa
authors.forename: N
authors.initials: N
authors.lastname: Okada
authors.forename: M
authors.initials: M
authors.lastname: Yamada
authors.forename: M
authors.initials: M
authors.lastname: Yoshikawa
authors.forename: M
authors.initials: M
authors.lastname: Tokuda
authors.forename: I
authors.initials: I
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Koga
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000737:chemistry*; D015252:Deoxyribonucleases, Type II Site-Specific; D004586:Electrophoresis; D005798:Genes, Bacterial; D005819:Genetic Markers; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D013295:Streptococcus mutans / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D005819:Genetic Markers; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases