Receptors for interleukin 4, interleukin 5 and interleukin 6.
cDNA encoding IL-4, IL-5 and IL-6 have been isolated and the availability of recombinant proteins allowed the demonstration of the pleiotropic effects of these cytokines. IL-4, IL-5 and IL-6 bind to high affinity receptors (Kd = 1-6 x 10(-10) M) expressed in low numbers on cells. The IL-4R is expressed on virtually every cell type whereas the IL-6R and IL-5R are less widely distributed. Low affinity IL-6 and IL-5 binding sites have also been detected but the biological activity of these different cytokines appears to correlate with the presence of high affinity binding sites. Biochemical characterization of the IL-4R, IL-5R and IL-6R by crosslinking of the labelled ligands, immunoprecipitation or purification experiments suggests that these receptors are composed of several proteins. IL-4 binds to three species (130, 75 and 65 kDa) and cDNAs encoding the 130 kDa protein have been isolated. IL-6 binds to a 80 kDa protein for which a cDNA has been isolated. Furthermore the IL-6 80 kDa protein complex binds to a 130 kDa transducer for which a cDNA was recently isolated. The IL-5R consists of three proteins of 105, 75 and 60 kDa but corresponding cDNAs have not yet been isolated. The IL-4, IL-6R 80 kDA protein and the IL-6 transducer protein display significant homology in their extracellular domains. These proteins belong to the new cytokine receptor family (hematopoietin receptors superfamily) which also includes the G-CSFR, GM-CSFR, IL-3, IL-7R and IL-2R 70 kDa chain.
Banchereau
J
J
Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
Galizzi
J P
JP
D000818:Animals; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D015847:Interleukin-4 / Q000378:metabolism* / Q000502:physiology; D015848:Interleukin-5 / Q000378:metabolism* / Q000502:physiology; D015850:Interleukin-6 / Q000378:metabolism* / Q000502:physiology; D008024:Ligands; D051379:Mice; D005810:Multigene Family; D009928:Organ Specificity; D011485:Protein Binding; D011971:Receptors, Immunologic / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D018123:Receptors, Interleukin; D019948:Receptors, Interleukin-4; D053649:Receptors, Interleukin-5; D019947:Receptors, Interleukin-6; D011975:Receptors, Mitogen / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D015848:Interleukin-5; D015850:Interleukin-6; D008024:Ligands; D011971:Receptors, Immunologic; D018123:Receptors, Interleukin; D019948:Receptors, Interleukin-4; D053649:Receptors, Interleukin-5; D019947:Receptors, Interleukin-6; D011975:Receptors, Mitogen; D015847:Interleukin-4; D004247:DNA