Factors affecting 39K NMR detectability in rat tissue.
In this study we have found that NMR detectability of 39K in rat thigh muscle may be substantially higher (up to 100% of total tissue potassium) than values previously reported of around 40%. The signal was found to consist of two superimposed components, one broad and one narrow, of approximately equal area. Investigations involving improvements in spectral parameters such as signal-to-noise ratio and baseline roll, together with computer simulations of spectra, show that the quality of the spectra has a major effect on the amount of signal detected, which is largely due to the loss of detectability of the broad signal component. In particular, lower-field spectrometers using conventional probes and detection methods generally have poorer signal-to-noise and worse baseline roll artifacts, which make detection of a broad component of the muscle signal difficult.
Rashid
S A
SA
School of Pharmaceutical Chemistry, Victorian College of Pharmacy Ltd, Parkville, Australia.
Adam
W R
WR
Craik
D J
DJ
Shehan
B P
BP
Wellard
R M
RM
D000818:Animals; D003198:Computer Simulation; D004867:Equipment Design; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods; D008954:Models, Biological; D009132:Muscles / Q000737:chemistry*; D010783:Photometry / Q000379:methods; D011188:Potassium / Q000032:analysis* / Q000737:chemistry; D051381:Rats; D013997:Time Factors
D011188:Potassium