Dipyridamole--evaluation of an established antithrombotic drug in view of modern concepts of blood cell-vessel wall interactions.
The effect of dipyridamole on the local antithrombotic activities of endothelium has been evaluated. Human whole blood was allowed to flow over an endothelial cell-derived extracellular matrix partially covered by human endothelial cells. Half-maximal suppression of platelet aggregate formation occurred with approximately 5 microM dipyridamole. Similarly, a pronounced inhibition of thrombus formation was observed by in vivo microscopy and computer-assisted morphometric analysis, after oral treatment of non-anesthetized hamsters with dipyridamole, 5 mg/kg. This strong suppression of thrombus formation was maintained in animals on a long-term cholesterol-supplemented diet. The antithrombotic potential of dipyridamole has been clearly demonstrated, both in vitro and in vivo using these more complex approaches employing quantitative microscopy.
Eisert
W G
WG
Department of Pharmacology, Dr K. Thomae GmbH, Biberach, FRG.
Müller
T H
TH
D000818:Animals; D001808:Blood Vessels / Q000293:injuries; D002791:Cholesterol, Dietary; D006224:Cricetinae; D004176:Dipyridamole / Q000494:pharmacology*; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008647:Mesocricetus; D010973:Platelet Adhesiveness / Q000187:drug effects; D013927:Thrombosis / Q000209:etiology / Q000517:prevention & control*
D002791:Cholesterol, Dietary; D005343:Fibrinolytic Agents; D004176:Dipyridamole