Determination of ximoprofen and its metabolites in human urine by high-performance liquid chromatography with ultraviolet absorbance detection.
A simple, sensitive and selective method for the determination of ximoprofen and its keto and hydroxy metabolites in human urine has been developed using high-performance liquid chromatography in the reversed-phase mode. The limit of reliable determination of ximoprofen and each of its metabolites in urine is about 1 microgram/ml (4 nmol/ml). The method has been applied to urine samples obtained from human volunteers after administration of single intravenous doses of 30 mg of ximoprofen and about 70% dose was accounted for in terms of these compounds and their glucuronic acid conjugates.
Morris
G R
GR
Department of Metabolism and Pharmacokinetics, Huntingdon Research Centre Ltd., U.K.
Walmsley
L M
LM
Brodie
R R
RR
Chasseaud
L F
LF
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000378:metabolism / Q000652:urine*; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D005966:Glucuronidase; D006207:Half-Life; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007275:Injections, Intravenous; D010666:Phenylpropionates / Q000378:metabolism / Q000652:urine*; D013056:Spectrophotometry, Ultraviolet
D000894:Anti-Inflammatory Agents, Non-Steroidal; D010666:Phenylpropionates; D005966:Glucuronidase; C061944:ximoprofen