Flavonoids as inhibitors of rat liver cytosolic glutathione S-transferase.
The inhibitory potencies of different flavonoids for rat liver cytosolic glutathione S-transferase activity varied over 30-fold, depending on the pattern of hydroxylation, the presence of a C-2, C-3 double bond and the substitution of a hydroxyl group with a sugar moiety. Kinetic inactivation studies of the enzyme with the inhibitor quercetin revealed a non-competitive profile versus both glutathione and 1-chloro-2,4-dinitrobenzene.
Merlos
M
M
Laboratory of Pharmacology and Pharmacognosy, School of Pharmacy, University of Barcelona, Spain.
Sanchez
R M
RM
Camarasa
J
J
Adzet
T
T
D000818:Animals; D003600:Cytosol / Q000201:enzymology; D004137:Dinitrochlorobenzene / Q000494:pharmacology; D005419:Flavonoids / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors*; D006900:Hydroxylation; D007700:Kinetics; D008099:Liver / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D015394:Molecular Structure; D011794:Quercetin / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013329:Structure-Activity Relationship
D004137:Dinitrochlorobenzene; D005419:Flavonoids; D011794:Quercetin; D005982:Glutathione Transferase; D005978:Glutathione