title: The effects of scleroderma sera on endothelial cell survival in vitro.
abstract: The effects of sera and of platelet-poor plasma from patients with scleroderma on endothelial cell survival in vitro were studied. The survival ratio of rat heart endothelial cells was studied both in 10% test serum and in 10% platelet-poor plasma. Sera from patients with scleroderma decreased the survival ratio significantly when compared with sera from normal controls. In contrast, there was no significant difference between platelet-poor plasma from patients with scleroderma and that from normal controls. Our data indicate that platelets in the patients with scleroderma may cause vascular damage by affecting endothelial cell survival.
authors.affiliation: Department of Dermatology, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Etoh
authors.forename: A
authors.initials: A
authors.lastname: Igarashi
authors.forename: K
authors.initials: K
authors.lastname: Iozumi
authors.forename: Y
authors.initials: Y
authors.lastname: Ishibashi
authors.forename: K
authors.initials: K
authors.lastname: Takehara
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D000974:Antibodies, Antinuclear / Q000032:analysis; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D002503:Centromere / Q000276:immunology; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D008875:Middle Aged; D009500:Neutralization Tests; D051381:Rats; D012595:Scleroderma, Systemic / Q000097:blood*; D016212:Transforming Growth Factor beta / Q000276:immunology
chemical_list: D000974:Antibodies, Antinuclear; D016212:Transforming Growth Factor beta