Phylogenetic comparisons of the branched-chain alpha-ketoacid dehydrogenase complex.
1. Antibodies against the E1b and E2b components of bovine branched-chain alpha-ketoacid (BCKA) dehydrogenase (BCKAD) complex completely inhibited BCKA oxidation in mammalian and avian mitochondria. BCKA oxidation by salmonid mitochondria was less affected and the enzyme from Pseudomonas putida was unaffected. 2. In rodents, anti-E1b E2b IgG inhibited oxidation of all three BCKA in a similar dose-dependent manner: oxidation of alpha-ketobutyrate and alpha-keto-y-methiolbutyrate was also partially inhibited. 3. Except for the salmonid BCKAD, a similar Mr for the E2b and E1b alpha proteins was observed in these species. 4. After digestion with V-8 protease similar immunoreactive peptides were observed for the human and rodent complex.
Eisenstein
R S
RS
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706.
Miller
R H
RH
Hoganson
G
G
Harper
A E
AE
D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002417:Cattle; D002645:Chickens; D015151:Immunoblotting; D007658:Ketone Oxidoreductases / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D010802:Phylogeny; D051381:Rats; D011919:Rats, Inbred Strains; D012697:Serine Endopeptidases / Q000378:metabolism; D012756:Sheep; D013045:Species Specificity; D014337:Trout
D000906:Antibodies; D009097:Multienzyme Complexes; D010446:Peptide Fragments; D007658:Ketone Oxidoreductases; D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase