title: Effects of selective cardiac denervation on collateral blood flow after coronary artery occlusion in conscious dogs.
abstract: The extent to which cardiac nerves regulate responses to myocardial ischemia remains controversial. Our data in conscious dogs indicate that neither selective posterior left ventricular (LV) wall denervation nor selective ventricular denervation, leaving the atria intact, modifies the effects of coronary artery occlusion (for 24 h) on regional myocardial function and infarct size as compared to normally innervated dogs. Since hemodynamic changes were similar among the three groups after coronary artery occlusion, it is possible to speculate that responses of collateral blood flow to the ischemic zone were also not modified by chronic selective cardiac denervation. To address this, individual samples were selected and included in either the infarcted (TTC negative) or salvaged (TTC positive) group. The infarcted and salvaged samples were paired according to blood flow levels of 0.1-0.2, 0.2-0.3, or 0.3-0.4 ml/min/g at either 5 min, 1 h, 3 h, or 6 h after coronary artery occlusion. The results demonstrated similar patterns of myocardial blood flow in tissue samples within the area at risk after coronary artery occlusion in the animals, regardless of whether the ischemic zone was innervated or denervated. While blood flow rose in ischemic tissue that ultimately was salvaged, and tended not to rise over the 24 h monitoring period in tissue samples that became necrotic, no differences could be discerned on the basis of intact or absent innervation of the ischemic zone. Thus, chronic absence of cardiac nerves does not affect regulation of ischemic zone blood flow following coronary artery occlusion in conscious dogs.
authors.affiliation: Department of Medicine, Harvard Medical School, Brigham & Women's Hospital, Southborough, Massachusetts.
authors.forename: Y T
authors.initials: YT
authors.lastname: Shen
authors.forename: D R
authors.initials: DR
authors.lastname: Knight
authors.forename: J X
authors.initials: JX
authors.lastname: Thomas
authors.forename: S F
authors.initials: SF
authors.lastname: Vatner
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology; D003097:Collateral Circulation / Q000502:physiology*; D003324:Coronary Artery Disease / Q000503:physiopathology*; D003326:Coronary Circulation / Q000502:physiology*; D003327:Coronary Disease / Q000503:physiopathology; D003714:Denervation; D004285:Dogs; D006321:Heart / Q000294:innervation; D009203:Myocardial Infarction / Q000503:physiopathology