A method for the in vitro determination of feline corneal endothelial permeability.
A mounting block for in vitro perfusion of the cat cornea is described. Using this apparatus and the techniques of Araie, the permeability (Pac) of the normal cat corneal endothelium to carboxyfluorescein was determined to be 2.5 +/- 0.2 x 10(-4) cm/min. To assess the sensitivity of this technique in determining changes in Pac associated with alterations in endothelial, morphology, three cats underwent 2 successive unilateral, central, 10 mm diameter circular areas of endothelial debridement 6 weeks apart. Six weeks following the second wounding all 3 animals underwent morphometric analysis and Pac determination. A trend toward an elevation in Pac with extreme reductions in cell density was observed.
McDermott
M L
ML
Department of Ophthalmology, Wayne State University, Detroit, MI.
Watsky
M A
MA
Geroski
D H
DH
Edelhauser
H F
HF
D000818:Animals; D001692:Biological Transport; D002415:Cats; D002452:Cell Count; D002463:Cell Membrane Permeability; D004195:Disease Models, Animal; D004728:Endothelium, Corneal / Q000293:injuries / Q000378:metabolism* / Q000648:ultrastructure; D005452:Fluoresceins / Q000493:pharmacokinetics; D066298:In Vitro Techniques; D010477:Perfusion / Q000379:methods
D005452:Fluoresceins; C024098:6-carboxyfluorescein