Preparative separation of pyrogens from proteins by isoelectric focusing using a multicompartment electrolyser with Immobiline membranes.
Due to the nature of lipopolysaccharide endotoxin structures of bacterial pyrogens, their removal from solutions containing therapeutic proteins is often a problem in the pharmaceutical industry. In this report we describe the application of electromotive force to dislodge lipopolysaccharide endotoxins from proteins. This was performed by employing a multicompartment electrolyzer fitted with Immobiline membranes of specified pIs. A thousand-fold reduction of endotoxin could be achieved in the model test system described. This contribution describes the use of a new recycling isoelectric focusing approach without the use of carrier ampholytes.
Lucas
J
J
Ciba-Geigy Biotechnology Service, Basel.
Faupel
M
M
Goecking
C
C
D007525:Isoelectric Focusing; D007526:Isoelectric Point; D008567:Membranes, Artificial; D008954:Models, Biological; D011749:Pyrogens / Q000302:isolation & purification*
D008567:Membranes, Artificial; D011749:Pyrogens