title: Inhibitory effects of phospholipase D on chondrogenesis in vitro.
abstract: Mesenchymal cells from the wing buds of stage 24 chick embryos undergo differentiation to cartilage when plated at high density. Treatment of these cultures with phospholipase D resulted in inhibition of chondrogenesis. Phospholipase D treatment (which produces phosphatidic acid from membrane phospholipids) was found to affect cell proliferation and to dramatically increase intracellular free calcium levels and inositol phosphate production. Intracellular free Ca2+, mobilized as a result of phosphatidylinositol phosphate hydrolysis, may therefore inhibit chondrogenesis in embryonic mesenchymal cells.
authors.affiliation: Department of Biology, University of Dayton, Ohio 45469-2320.
authors.forename: P A
authors.initials: PA
authors.lastname: Tsonis
authors.forename: K
authors.initials: K
authors.lastname: Del Rio-Tsonis
authors.forename: J
authors.initials: J
authors.lastname: Rothrock
authors.forename: J
authors.initials: J
authors.lastname: Dominguez
authors.forename: D
authors.initials: D
authors.lastname: English
authors.forename: K
authors.initials: K
authors.lastname: Glade
authors.forename: P F
authors.initials: PF
authors.lastname: Goetinck
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002356:Cartilage / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D007295:Inositol Phosphates / Q000378:metabolism; D010739:Phospholipase D / Q000494:pharmacology*
chemical_list: D007295:Inositol Phosphates; D010739:Phospholipase D; D002118:Calcium