Non-radioactive labeling and detection of nucleic acids. II. Optimization of the digoxigenin system.
The random-primed DNA labeling technique was modified for the incorporation of digoxigenin into DNA as basic component of the digoxigenin-based non-radioactive DNA labeling and detection system. Digoxigenin molecules act as reporter groups for highly sensitive DNA detection by a digoxigenin-specific antibody:alkaline phosphatase-conjugate-catalysed color reaction. The parameters affecting the individual reaction steps of the digoxigenin labeling, hybridization and detection reactions were optimized to maximal sensitivity and specificity.
Höltke
H J
HJ
Boehringer Mannheim GmbH, Biochemisches Forschungszentrum Penzberg, Tutzing.
Seibl
R
R
Burg
J
J
Mühlegger
K
K
Kessler
C
C
D015152:Blotting, Northern; D015139:Blotting, Southern; D003101:Collodion; D004247:DNA / Q000032:analysis* / Q000378:metabolism; D015342:DNA Probes; D004076:Digoxigenin / Q000378:metabolism; D015151:Immunoblotting; D008567:Membranes, Artificial; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000032:analysis*; D009757:Nylons
D015342:DNA Probes; D008567:Membranes, Artificial; D009696:Nucleic Acids; D009757:Nylons; D003101:Collodion; D004247:DNA; D004076:Digoxigenin