title: [The detection of viral antigens and ribosomal RNA in cells by using low-temperature media].
abstract: A post-embedding technique for immunocytochemical analysis at the ultrastructural level was used to detect and localize HIV antigens on ultrathin sections of Lowicryl-embedded HIV-infected cells. A genomic probe containing ribosomal sequences and labeled with biotin was used to hybridize rRNA molecules in sections of animal cells embedded in Lowicryl. The method presently described offers the possibility to detect rapidly and precisely ribosomal gene expression and viral proteins at the ultrastructural level.
authors.forename: V B
authors.initials: VB
authors.lastname: Grigor'ev
authors.forename: Iu P
authors.initials: IuP
authors.lastname: Kadoshnikov
authors.forename: F
authors.initials: F
authors.lastname: Escaig
authors.forename: J G
authors.initials: JG
authors.lastname: Fournier
authors.forename: I A
authors.initials: IA
authors.lastname: Rudneva
authors.forename: E V
authors.initials: EV
authors.lastname: Karamov
authors.forename: E V
authors.initials: EV
authors.lastname: Kazennova
authors.forename: M M
authors.initials: MM
authors.lastname: Garaev
mesh_terms: D000180:Acrylic Resins; D000818:Animals; D015342:DNA Probes; D005786:Gene Expression Regulation / Q000235:genetics; D015488:HIV Antigens / Q000032:analysis*; D015497:HIV-1 / Q000276:immunology*; D015498:HIV-2 / Q000276:immunology*; D006801:Humans; D008722:Methods; D009693:Nucleic Acid Hybridization; D012335:RNA, Ribosomal / Q000032:analysis* / Q000235:genetics; D013696:Temperature
chemical_list: D000180:Acrylic Resins; D015342:DNA Probes; D015488:HIV Antigens; D012335:RNA, Ribosomal; C037883:Lowicryl 4KM resin
vernacular_title: Vyiavlenie virusnykh antigenov i ribosomal'noĭ RNK v kletkakh s ispol'zovaniem nizkotemperaturnykh sred.