[Oxygen activation by lymphocytes during interaction of HLA antibodies with HLA antigens].
The method of luminol-dependent chemiluminescence was used to detect oxygen activation during interaction of HLA antibodies with HLA antigens expressed on lymphocyte surface. Introduction of specific anti-HLA serum into the lymphocyte suspension leads to a rapid decrease of intensity in chemiluminescence intensity follows. The maximum yield of induced chemiluminescence in case of using a specific antiserum is significantly lower than in the control. The method could be used for detection of HLA antigens on lymphocyte surface.
Semenkova
G N
GN
Levin
V I
VI
Cherenkevich
S N
SN
Svirnovskiĭ
A I
AI
Medvedeva
I N
IN
Voĭtkun
V A
VA
D000937:Antigen-Antibody Reactions / Q000502:physiology*; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D003470:Culture Media; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D007518:Isoantibodies / Q000276:immunology*; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D010100:Oxygen / Q000097:blood*
D003470:Culture Media; D006680:HLA Antigens; D007518:Isoantibodies; D008165:Luminol; D010100:Oxygen
Aktivatsiia kisloroda limfotsitami pri vzaimodeĭstvii HLA-antitel s HLA-antigenami.