Cytological effects of culture media conditioned by B16 melanoma cells and 3T3 fibroblasts.
Culture media conditioned (CM) by mixed populations of mouse B16 melanoma cells and 3T3 fibroblasts cultivated as monolayers exert cytological effects on B16 or 3T3 cells when treated separately in culture. By ultrafiltration of these CM, we show that a stimulatory activity on B16 melanoma cells proliferation is present in fractions with M.W. greater than 10,000 daltons. A strong cytotoxic activity for B16 melanoma cells and, to a lower degree, for 3T3 fibroblasts is detected in fractions with M.W. less than 1,000 daltons. The ultrastructural analysis of cells (B16 or 3T3) treated with cytotoxic fractions reveals in them mitochondrial swelling, blebs, broken membranes and dead cells.
Coucke
P H
PH
Department of Histology and Cytology, Faculty of Medicine, University of Liège, Belgium.
Siwek
B L
BL
De Pauw-Gillet
M C
MC
Bassleer
R J
RJ
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D006131:Growth Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D007700:Kinetics; D008546:Melanoma, Experimental / Q000473:pathology / Q000503:physiopathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000302:isolation & purification / Q000494:pharmacology; D008970:Molecular Weight; D014462:Ultrafiltration
D003470:Culture Media; D006131:Growth Inhibitors; D008934:Mitogens