title: Influence of dietary fatty acid composition on cholesterol synthesis and esterification in hamsters.
abstract: To investigate the effects of dietary fat quality on synthesis and esterification of cholesterol, Syrian hamsters were fed diets containing corn, olive, coconut or menhaden oils (10% w/w) with added cholesterol (0.1% w/w). After 3 weeks, animals were sacrificed 90 min following IP injection of 3H2O. Synthesis of free cholesterol and movement of free cholesterol into ester pools were measured from 3H-uptake rate in liver and duodenum. Plasma total cholesterol and triglycerides levels were highest in coconut oil-fed animals, whereas hepatic total cholesterol and ester levels were elevated in olive oil-fed animals, as compared with all other groups. No diet-related differences were seen in duodenal cholesterol or total fatty acid content. In duodenum, uptake of 3H per g tissue into cholesterol was greater compared with liver; however, within each tissue, 3H-uptake into cholesterol was similar across groups. Notably, 3H-uptake into cholesterol ester in liver was highest in menhaden oil-fed animals. These data suggest that menhaden fish oil consumption results in enhanced movement of newly synthesized cholesterol into ester as compared with other fat types.
authors.affiliation: Division of Human Nutrition, School of Family and Nutritional Sciences, University of British Columbia, Vancouver, Canada.
authors.forename: P J
authors.initials: PJ
authors.lastname: Jones
authors.forename: J E
authors.initials: JE
authors.lastname: Ridgen
authors.forename: A P
authors.initials: AP
authors.lastname: Benson
mesh_terms: D000818:Animals; D001835:Body Weight; D002784:Cholesterol / Q000096:biosynthesis* / Q000378:metabolism; D006224:Cricetinae; D004041:Dietary Fats / Q000032:analysis*; D004386:Duodenum / Q000502:physiology; D004952:Esters / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis*; D005766:Gastrointestinal Contents / Q000737:chemistry; D008055:Lipids / Q000032:analysis / Q000097:blood; D008297:Male; D008647:Mesocricetus; D009929:Organ Size
chemical_list: D004041:Dietary Fats; D004952:Esters; D005227:Fatty Acids; D008055:Lipids; D002784:Cholesterol