Polymer-protein conjugates. I. Effect of protein conjugation on the cloud point of poly (N-isopropylacrylamide).
Poly (N-isopropylacrylamide) (p(NIPAAm] exhibits a cloud point around 32 degrees C. In this study we have conjugated several proteins to p(NIPAAm), and the effects of protein molecular weight, pH and ionic strength on the cloud point of the p(NIPAAm)-protein conjugates have been studied. The cloud points of p(NIPAAm)-protein conjugates appear to be unaffected by the molecular weight or isoelectric point (pl) of the proteins, the pH or ionic strength.
Chen
J P
JP
Center for Bioengineering, FL-20, University of Washington, Seattle 98195.
Yang
H J
HJ
Hoffman
A S
AS
D000180:Acrylic Resins / Q000737:chemistry*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003692:Delayed-Action Preparations / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008970:Molecular Weight; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
D000180:Acrylic Resins; D003692:Delayed-Action Preparations; D011108:Polymers; D011506:Proteins; C052970:poly-N-isopropylacrylamide