Determination of the components of analgesic mixtures using high-performance thin-layer chromatography.
A spectrodensitometric method is described for the determination of the components of two analgesic mixtures. For the first mixture (paracetamol-ascorbic acid-caffeine-phenylephrine), the pharmaceutically active components were separated from each other and closely related degradation products and impurities on high-performance thin-layer chromatography (F254) plates using methylene chloride-ethyl acetate-ethanol-formic acid (3.5 + 2 + 4 + 0.5) and methylene chloride-ethyl acetate-ethanol (5 + 5 + 1) as the developing systems. The other mixture (phenazone-phenacetin-caffeine) was separated efficiently from the degradation products using the same plates and acetonitrile-chloroform (1 + 1) as the mobile phase. The proposed method was used to determine these mixtures in commercial tablets.
el Sadek
M
M
Faculty of Pharmacy, Zagazig University, Egypt.
el Shanawany
A
A
Aboul Khier
A
A
Rücker
G
G
D000082:Acetaminophen / Q000032:analysis; D000700:Analgesics / Q000032:analysis*; D000983:Antipyrine / Q000032:analysis; D001205:Ascorbic Acid / Q000032:analysis; D002110:Caffeine / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D010615:Phenacetin / Q000032:analysis; D010656:Phenylephrine / Q000032:analysis
D000700:Analgesics; D010656:Phenylephrine; D000082:Acetaminophen; D002110:Caffeine; D010615:Phenacetin; D001205:Ascorbic Acid; D000983:Antipyrine