[Induction by kopsinine of hepatic mixed-function oxidase in mice].
Kopsinine is an indole alkaloid. Oral administration of kopsinine 200 mg/kg once daily for 3 d significantly increased liver microsomal protein, cytochrome P-450, cytochrome b5, NADPH-cytochrome C reductase, aminopyrine demethylase and benzo(a)pyrine hydroxylase activities in mice. kopsinine only induced cytochrome P-450 in rough endoplasmic reticulum of liver. SDS-polyamine gel electrophoresis analysis showed that the protein bands of microsomes from kopsinine treated mice were similar to that induced by phenobarbital in mice. Metyrapon, a specific inhibitor of cytochrome P-450, partially antagonized aminopyrine demethylase activity of microsomes from mice treated with kopsinine. The results suggest that kopsinine yields a pentobarbital-like induction on liver mixed function oxidase in mice. In addition, kopsinine was found to shorten the barbital-induced sleeping time in mice.
Huang
W Y
WY
Department of Pharmacology, Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Liu
G T
GT
D000284:Administration, Oral; D000470:Alkaloids / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis; D026121:Indole Alkaloids; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000096:biosynthesis*; D012890:Sleep / Q000187:drug effects
D000470:Alkaloids; D026121:Indole Alkaloids; C061491:kopsinine; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases