Antibodies to gut protozoa in commercial immunoglobulin preparations.
Using parasite agglutination, indirect immunofluorescence, enzyme-linked immunosorbent assay, and immunoblotting, Giardia lamblia specific antibody was detected in 5 commercially available immunoglobulin (IgG) preparations. IgG antibodies to Entamoeba histolytica were either present in very low titre or were absent. Immunoblotting showed that anti-G. lamblia antibodies were detected towards a range of Giardia proteins, 25-200 kDa. These findings raise the possibility that pooled human IgG preparations could be evaluated in the treatment of chronic giardiasis which is refractory to conventional therapy.
Cevallos
A M
AM
Department of Gastroenterology, St Bartholomew's Hospital, West Smithfield, London, UK.
Butcher
P D
PD
Arvind
A S
AS
Webster
A D
AD
Farthing
M J
MJ
D000371:Agglutination; D000818:Animals; D000913:Antibodies, Protozoan / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D004748:Entamoeba histolytica / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D005872:Giardia / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000276:immunology / Q000302:isolation & purification / Q000627:therapeutic use*
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G