Changes in the phospholipid and fatty acid composition in normal erythrocytes from sheep of different ages. Aminophospholipid organization in the membrane bilayer.
Development and aging processes in mammals are associated with changes in several physiological parameters. The aim of the present study was to investigate the changes in erythrocyte lipid composition during sheep development. In all the age groups studied, cholesterol/phospholipid ratios remained constant, at close to unity, while phospholipid patterns (sphingomyelin: 45-51%, phosphatidylethanolamine: 26-33%, phosphatidylserine: 13-19% and phosphatidylcholine: less than 2%) changed during development, with a statistically significant decrease (P less than 0.01) in phosphatidylserine and an increase in sphingomyelin content. These data suggest an increase in the rigidity of the erythrocyte lipid bilayer in adult sheep when compared with 1-month-old animals due to a decrease in the phosphatidylserine/sphingomyelin ratio. Fatty acid profiles consistently showed 5 main acids: oleic (52-54%), stearic (17-18%), linoleic (9-15%), palmitic (8.5-11%) and arachidonic acid (2-3%), mainly with significant variations (P less than 0.01) in palmitic and linoleic acid contents, respectively reaching the highest and lowest percentages in the youngest sheep. However, the developmental process seems to have no influence on the aminophospholipid topology of erythrocytes. This study suggests that the animals' developmental process has a marked effect on the lipid composition of erythrocyte membranes, which could affect cell functions.
Marin
M S
MS
Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Salamanca, Spain.
Fernandez
A
A
Sanchez-Yagüe
J
J
Cabezas
J A
JA
Llanillo
M
M
D000375:Aging / Q000502:physiology*; D000818:Animals; D004905:Erythrocyte Aging / Q000502:physiology*; D004910:Erythrocyte Membrane / Q000737:chemistry* / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D008051:Lipid Bilayers / Q000737:chemistry*; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000032:analysis / Q000737:chemistry*; D010718:Phosphatidylserines / Q000032:analysis; D010743:Phospholipids / Q000378:metabolism*; D012756:Sheep / Q000254:growth & development; D013109:Sphingomyelins / Q000032:analysis; D014302:Trinitrobenzenesulfonic Acid
D005227:Fatty Acids; D008051:Lipid Bilayers; D008563:Membrane Lipids; D010718:Phosphatidylserines; D010743:Phospholipids; D013109:Sphingomyelins; D014302:Trinitrobenzenesulfonic Acid