Purification and characterization of iduronic acid-rich and glucuronic acid-rich proteoglycans implicated in human post-burn keloid scar.
Small proteoglycans (PGs), extracted from human keloid scar tissue with 4M guanidinium chloride and fractionated by DEAE-cellulose chromatography, were separated by ethanol precipitation into one L-iduronic acid-rich and one D-glucuronic acid-rich fraction. The size of the L-iduronic acid-rich PG was 102 kDa with a 27 kDa glycosaminoglycan chain, that of the D-glucuronic acid-rich PG was 90 kDa with a 26 kDa glycosaminoglycan chain, and the protein core of both PGs was 14.5 kDa. The two PGs carried sulfate groups mostly attached at C-4 of the 2-amino-2-deoxy-D-galactose units. The N-terminal amino acid sequence of both was similar to human bone PGII (decorin), normal and hypertrophic scar, and human dermal tissue PG.
Garg
H G
HG
Department of Biological Chemistry, and Molecular Pharmacology, Harvard Medical School, Shriners Burns Institute, Boston, Massachusetts.
Lippay
E W
EW
Burd
D A
DA
Neame
P J
PJ
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001704:Biopolymers; D002056:Burns / Q000150:complications*; D004187:Disaccharides / Q000032:analysis; D004588:Electrophoresis, Cellulose Acetate; D004591:Electrophoresis, Polyacrylamide Gel; D005965:Glucuronates / Q000032:analysis*; D020723:Glucuronic Acid; D006025:Glycosaminoglycans / Q000032:analysis; D006801:Humans; D007067:Iduronic Acid / Q000032:analysis*; D007627:Keloid / Q000209:etiology / Q000378:metabolism*; D008969:Molecular Sequence Data; D011509:Proteoglycans / Q000737:chemistry* / Q000302:isolation & purification
D000596:Amino Acids; D001704:Biopolymers; D004187:Disaccharides; D005965:Glucuronates; D006025:Glycosaminoglycans; D011509:Proteoglycans; D007067:Iduronic Acid; D020723:Glucuronic Acid