Column chromatographic pre-concentration of iron(III) in alloys and biological samples with 1-nitroso-2-naphthol-3,6-disulphonate and benzyldimethyltetradecylammonium-perchlorate adsorbent supported on naphthalene using atomic absorption spectrometry.
The solid ion-pair material produced from the reaction between benzyldimethyltetradecylammonium chloride (BDTA) and sodium perchlorate on naphthalene provides the basis for a simple, rapid and selective technique for pre-concentrating iron from up to 500 ml of aqueous solution. Iron reacts with disodium 1-nitroso-2-naphthol-3,6-disulphonate (Nitroso-R salt) to form a water-soluble coloured chelate anion. The iron chelate anion forms a water-insoluble, stable iron-Nitroso-R-BDTA complex on naphthalene packed in a column. Trace amounts of iron are quantitatively retained on naphthalene in the pH range 3.5-7.5 and at a flow-rate of 1-2 ml min-1. The solid mass is dissolved out from the column with 5 ml of N,N-dimethylformamide and iron is determined by means of an atomic absorption spectrometer at 248 nm. The calibration graph is linear for concentrations of iron over the range of 0.5-20 micrograms in 5 ml of final solution. The standard deviation and relative standard deviation were calculated. The detection limit of the method was 0.0196 micrograms ml-1 of iron. The sensitivity for 1% absorption was 0.072 microgram ml-1 (0.165 microgram ml-1 by direct atomic absorption spectrometry of aqueous solution). The proposed method was applied to the determination of iron in standard alloys and biological samples.
Miura
J
J
Faculty of Engineering, Fukui University, Japan.
Arima
S
S
Satake
M
M
D000327:Adsorption; D000497:Alloys / Q000032:analysis*; D000818:Animals; D001548:Benzalkonium Compounds; D049872:Bivalvia / Q000032:analysis; D002708:Chlorella / Q000032:analysis; D002845:Chromatography / Q000379:methods*; D005290:Ferric Compounds / Q000032:analysis*; D007202:Indicators and Reagents; D009281:Naphthalenes; D009282:Naphthalenesulfonates; D009603:Nitroso Compounds; D013054:Spectrophotometry, Atomic
D000497:Alloys; D001548:Benzalkonium Compounds; D005290:Ferric Compounds; D007202:Indicators and Reagents; D009281:Naphthalenes; D009282:Naphthalenesulfonates; D009603:Nitroso Compounds; C001259:Fremy's salt; C005883:zephiramine; C031721:naphthalene; C004200:1-nitroso-3,6-disulfonate