Decondensation of mouse sperm chromatin in cell-free extracts: a micromethod.
A micromethod is presented which makes possible the analysis of mouse sperm nucleus decondensation in vitro using very small volumes of cytoplasmic preparations, even smaller than 1 microliters. We show that cell-free extracts obtained from interphase HeLa cells as well as lysates from mouse eggs and embryos can sustain early stages of mouse sperm nucleus transformation, provided the sperm nuclear envelope is damaged or removed.
Maleszewski
M
M
Department of Embryology, University of Warsaw, Poland.
D000818:Animals; D001399:Azure Stains; D002467:Cell Nucleus / Q000502:physiology / Q000648:ultrastructure; D002843:Chromatin / Q000502:physiology* / Q000648:ultrastructure; D003593:Cytoplasm / Q000502:physiology / Q000648:ultrastructure; D005307:Fertilization in Vitro; D006367:HeLa Cells; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013094:Spermatozoa / Q000502:physiology* / Q000648:ultrastructure
D001399:Azure Stains; D002843:Chromatin