Secondary cataract material collected with a glass cannula. A SEM study.
Secondary cataract material from three patients, collected with a glass cannula approx. 18, 24 and 30 months respectively after operation, was prepared for SEM examination. For the soft samples this was done by filtration through a millipore filter followed by fixation and drying. The more solid material was suspended in a fixation solution, followed by centrifuging, suspension in 70% ethanol and drying on a specimen-holder. The short residence samples (18 months) showed mainly erythrocytes, some (inflammatory) cells and degenerated lens-fibre material. Most of the more solid material, which was collected more than 20 months after operation, showed fragments of (regenerated) capsule epithelium and pieces of solid lens-fibre material with fragments of capsule epithelium attached.
Jongebloed
W L
WL
Department of Histology and Cellbiology, University of Groningen, The Netherlands.
van der Veen
G
G
Dijk
F
F
Worst
J G
JG
D002386:Cataract / Q000473:pathology*; D002387:Cataract Extraction; D006801:Humans; D007908:Lens, Crystalline / Q000648:ultrastructure*; D013997:Time Factors