title: Interaction of bovine lactoferrin with other proteins of milk whey.
abstract: The association between bovine lactoferrin and the major bovine whey proteins, beta-lactoglobulin, alpha-lactalbumin and albumin has been studied by immunochemical techniques, gel filtration and affinity chromatography in lacteal secretions and using purified proteins. Bovine lactoferrin is able to form non-covalent complexes with beta-lactoglobulin or albumin, with lactoferrin-protein molar ratios of 2:1 and 1:1 respectively. No association was detected with alpha-lactalbumin. Lactoferrin interacts with beta-lactoglobulin-Sepharose at low ionic strength, but not in the presence of 0.3 M NaCl, indicating that ionic interactions are important. The lack of association with alpha-lactalbumin suggests however a certain degree of specificity in this electrostatic interaction.
authors.affiliation: Departmento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Zaragoza, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Lampreave
authors.forename: A
authors.initials: A
authors.lastname: Piñeiro
authors.forename: J H
authors.initials: JH
authors.lastname: Brock
authors.forename: H
authors.initials: H
authors.lastname: Castillo
authors.forename: L
authors.initials: L
authors.lastname: Sánchez
authors.forename: M
authors.initials: M
authors.lastname: Calvo
mesh_terms: D000418:Albumins / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D005260:Female; D007122:Immunoelectrophoresis; D007768:Lactalbumin / Q000737:chemistry*; D007781:Lactoferrin / Q000737:chemistry*; D007782:Lactoglobulins / Q000737:chemistry*
chemical_list: D000418:Albumins; D007782:Lactoglobulins; D007768:Lactalbumin; D007781:Lactoferrin