Tartrate-resistant, purple acid phosphatase in Gaucher cells of the spleen. Immuno- and cytochemical analysis.
Bioptic material from the spleen of a three-year-old boy with a type 1 Gaucher disease was studied by immuno- and cytochemical methods with special regard to the macrophage-derived Gaucher cells. These cells were positive with PAS and Prussian blue staining, and were immuno-positive with the monoclonal 25 F9 antibody, specific to mature, non-inflammatory macrophages. Large Gaucher cells and their postulated small precursor cells revealed strong tartrate-resistant acid phosphatase (TRAP) and unspecific carboxylate esterase activities. Using a polyclonal antibody to bovine spleen purple phosphatase, a lysosomal TRAP from splenic macrophages, the TRAP of the Gaucher cells could be identified to belong to this group of iron-containing, purple acid phosphatases immunocytochemically. The origin of splenic Gaucher cells from blood monocytes and their further development are discussed.
Schindelmeiser
J
J
Institut für Anatomie, Westfälische Wilhelms-Universität Münster, FRG.
Radzun
H J
HJ
Münstermann
D
D
D000135:Acid Phosphatase / Q000032:analysis*; D002675:Child, Preschool; D005776:Gaucher Disease / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D013154:Spleen / Q000166:cytology / Q000201:enzymology*; D013644:Tartrates / Q000494:pharmacology
D013644:Tartrates; D000135:Acid Phosphatase; C029768:tartaric acid