Spatial normalization of one-dimensional electrophoretic gel images.
A strategy for using processed, digitized images of one-dimensional electrophoretic gels to facilitate the analysis of large sets of overlapping clones is described. The images are acquired from fluorescently stained gels or from transilluminated gel photographs using a cooled, solid-state charge-coupled device camera. By employing sets of bands in the size-standard lanes as reference points, all the gel images are spatially normalized to a common reference template. After normalization, lane images from different gels can be compared as though the gels had been electrophoresed under identical, uniform-field conditions. Applications of this procedure to the analysis of a large set of overlapping lambda clones from chromosome VII of Saccharomyces cerevisiae and to the estimation of fragment sizes are illustrated.
Drury
H A
HA
Biomedical Computer Laboratory, Washington University, St. Louis, Missouri 63110.
Green
P
P
McCauley
B K
BK
Olson
M V
MV
Politte
D G
DG
Thomas
L J
LJ
D000703:Analog-Digital Conversion; D015825:Chromosomes, Fungal; D004271:DNA, Fungal / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D004586:Electrophoresis; D005782:Gels; D012441:Saccharomyces cerevisiae / Q000235:genetics
D004271:DNA, Fungal; D004274:DNA, Recombinant; D005782:Gels