Determination of the protofilament number in microtubules by templates.
We have used microtubules (MTs) and double-walled microtubules (dwMTs), both fragmented, as templates for MT formation from phosphocellulose-purified tubulin. In both cases the mean protofilament number of the nucleated MTs corresponds to that of the templates. The results confirm the observations of Scheele et al. (J. Mol. Biol. 154, 485, 1982) that templates determine the protofilament number of nucleated MTs.
Vater
W
W
Central Institute of Microbiology and Experimental Therapy, Academy of Sciences of the GDR, Jena.
Böhm
K J
KJ
Steinmetzer
P
P
Unger
E
E
D008841:Actin Cytoskeleton / Q000648:ultrastructure; D000818:Animals; D001921:Brain / Q000378:metabolism / Q000648:ultrastructure; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D008868:Microtubule Proteins / Q000302:isolation & purification / Q000648:ultrastructure*; D008870:Microtubules / Q000648:ultrastructure*; D013552:Swine
D046911:Macromolecular Substances; D008868:Microtubule Proteins