[Hepatitis B virus DNA. Current diagnosis in children with chronic hepatitis B].
After cloning the hepatitis B virus genome molecular hybridization techniques have been established for detection of HBV-DNA in serum and liver tissue. HBV-DNA is presently the most sensitive marker of viral replication and infectivity, which was previously related to the presence of HBeAg in serum and HBcAg in liver cells. HBV-DNA is usually analysed by dot blot-, Southern blot- and in situ hybridization techniques. The study of HBV-DNA has become a valuable part of the routine diagnostic in chronic hepatitis B, providing a more reliable estimation of viral replication and contagiousness and better parameters for severity and prognosis of the chronic infection.
Wirth
S
S
Kinderklinik, Johannes-Gutenberg-Universität Mainz.
Keller
K M
KM
Baumann
W
W
Zabel
B
B
D015139:Blotting, Southern; D002648:Child; D015342:DNA Probes; D005814:Genes, Viral / Q000235:genetics; D006509:Hepatitis B / Q000175:diagnosis*; D006515:Hepatitis B virus / Q000235:genetics*; D006521:Hepatitis, Chronic / Q000175:diagnosis*; D006801:Humans; D015151:Immunoblotting
D015342:DNA Probes
Hepatitis-B-Virus-DNS. Aktuelle Diagnostik bei Kindern mit chronischer Hepatitis B.