In situ detection of cutaneous melanoma by prompt gamma-ray spectrometry using melanoma-seeking 10B-dopa analogue.
10B1-para-boronophenylalanine (10B1-BPA), one of our boronated dopa analogues developed for thermal neutron capture therapy, has been found to have a selective affinity for malignant melanoma. We have established a method of 'in situ' detection of subcutaneous melanoma lesions, using this melanoma-seeking 10B-labeled compound. In this study, we applied an 'in situ' 10B microanalysis system via detection of the prompt gamma-ray from the 10B(n, alpha)7Li reaction triggered by irradiating the 10B-containing target with pure thermal neutrons, called prompt gamma-ray spectrometry, to hamsters bearing Greene's melanoma in subcutis and to a human patient whose occipital subcutaneous tumor was suspected of being a metastatic melanoma. In the hamsters, the time-dependent 10B dynamics showed increased 10B accumulation in melanoma, after 10B1-BPA administration, in contrast to that in non-melanoma normal skin. In the human patient, after subcutaneous injection of 10B1-BPA into perilesional sites 4 cm distant from the tumor margin, the average 10B concentration in the tumor was determined to be 24 ppm (microgram/g), in contrast to 3 ppm in skin covering the tumor and 1.1 ppm in blood, indicative of selectively high 10B1-BPA uptake by the tumor.
Honda
C
C
Department of Dermatology, Kobe University School of Medicine, Japan.
Mishima
Y
Y
Ichihashi
M
M
Hatta
S
S
D000368:Aged; D000818:Animals; D001895:Boron / Q000378:metabolism; D001896:Boron Compounds / Q000378:metabolism; D006224:Cricetinae; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007554:Isotopes; D008297:Male; D008545:Melanoma / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D008647:Mesocricetus; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000378:metabolism; D012878:Skin Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D013051:Spectrometry, Gamma / Q000379:methods; D013997:Time Factors
D001896:Boron Compounds; D007554:Isotopes; D010649:Phenylalanine; D001895:Boron; C033685:4-boronophenylalanine