Procedure for the purification of streptavidin by hydrophobic interaction chromatography.
A procedure is described for the purification of hydrophobic microbial proteins such as streptavidin from Streptomyces avidinii, using Benzyl-DC bead cellulose as the column material. The separation is rapid with a high loading capacity and sufficient resolution for preparative uses. Advantages are discussed especially for industrial purposes.
Schwidop
W D
WD
VEB Chemieanlagenbaukombinat Leipzig-Grimma, HA Biotechnologie, Leipzig, G.D.R.
Klossek
P
P
Müller
R
R
Claus
R
R
D001426:Bacterial Proteins / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D019809:Streptavidin; D013302:Streptomyces / Q000032:analysis / Q000166:cytology
D001426:Bacterial Proteins; D019809:Streptavidin