The length polymorphism in the 5' flanking region of the human beta-globin gene with denaturing gradient gel electrophoresis in a Japanese population.
The ATTTT repeat polymorphism located approximately 1,400 base pairs (bp) upstream from the beta-globin structural gene was analyzed by denaturing gradient gel electrophoresis (DGGE) of RNA:DNA duplexes. A study of 81 unrelated Japanese from Hiroshima revealed a sequence heteromorphism in this site. The alleles with five and six repeats of the ATTTT unit, which have been reported, were found in polymorphic proportions. Two unreported alleles were also detected, the first, in two persons, characterized by seven repeats and the other, in a single person, having an A-to-G nucleotide substitution in the fifth repeat.
Takahashi
N
N
Department of Genetics, Radiation Effects Research Foundation, Hiroshima, Japan.
Hiyama
K
K
Kodaira
M
M
Satoh
C
C
D002460:Cell Line; D004586:Electrophoresis; D005914:Globins / Q000235:genetics*; D007564:Japan; D010957:Plasmids; D011110:Polymorphism, Genetic
D005914:Globins