[The identification of Ca2+-dependent nucleases activated in the nuclei of rat thymocytes under the action of glucocorticoids].
Spectra of thymocyte's nuclear nucleases of control and glucocorticoid treated (5 mg/kg body weight) adrenalectomized rats have been investigated. Using the method of SDS-electrophoresis of nuclear proteins in 3H-DNA-polyacrylamide gel (PAAG) the authors managed to discover a number of polypeptides of 35, 32, 17.7, 17.0, 16.4 kDa molecular mass possessing a nuclease activity. The enzyme of 35 kDa is only active in the presence of Ca2+ and Mg2+ ions and inhibited by cycloheximide. Nucleases of 32, 17.7, 17.0, 16.4 kDa are active in the presence of Ca2+ ions. The enzymic activity of these nucleases increases 60 min after steroid treatment. Nuclease of 17.7, 17.0, 16.4 kDa are poly(ADP-ribosylated). Glucocorticoid mediated activation don't blocked by poly(ADP-ribosylation). Possible role and mechanism of discovered nucleases are discussed.
Seĭliev
A A
AA
Khanson
K P
KP
D000315:Adrenalectomy; D000818:Animals; D002118:Calcium / Q000032:analysis*; D002467:Cell Nucleus / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology*; D002852:Chromatography, Ion Exchange; D003851:Deoxyribonucleases / Q000032:analysis* / Q000187:drug effects / Q000302:isolation & purification; D003907:Dexamethasone / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D015151:Immunoblotting; D008297:Male; D008970:Molecular Weight; D051381:Rats; D013950:Thymus Gland / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology*
D003907:Dexamethasone; D003851:Deoxyribonucleases; D002118:Calcium
Identifikatsiia Ca2+-zavisimykh nukleaz, aktiviziruiushchikhsia v iadrakh timotsitov krys pod deĭstviem gliukokortikoidov.