title: Isolation of a serologically different compact-colony-forming active substance from strains of Staphylococcus aureus.
abstract: To observe the possible serological heterogeneity of compact-colony-forming active substance (CCFAS), heat-killed vaccines were prepared by two strains of Staphylococcus aureus, strains SMU 1-46 and SMU 7931, cultured in 0.03 M trishydrochloride-buffered brain heart infusion, pH 8.4. After immunization with the vaccine in rabbits, antibody responses were observed during a period of six weeks after the immunization either by homologous and heterologous organisms using alkaline serum-soft agar technique. The results showed that remarkable antibody production was shown only against homologous strain, but not against heterologous strain. The antibodies were absorbed out only with highly purified preparation of CCFAS extracted from homologous strain and not with heterologous CCFAS. Differences of the major chemical composition of the substances showed that highly purified CCFAS extracted from strain SMU 7931 contained 2.84 and 2.04 times higher amounts of galactose and 2-amino-2-deoxy-D-galacturonic acid than those of CCFAS obtained from strain SMU 1-46.
authors.affiliation: Department of Microbiology, St Marianna University School of Medicine, Kanagawa.
authors.forename: K
authors.initials: K
authors.lastname: Yoshida
authors.forename: T
authors.initials: T
authors.lastname: Ohtomo
authors.forename: M
authors.initials: M
authors.lastname: Suganuma
mesh_terms: D000362:Agar; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001428:Bacterial Vaccines; D004058:Diffusion; D005690:Galactose / Q000032:analysis; D006603:Hexuronic Acids / Q000032:analysis; D007106:Immune Sera; D011135:Polysaccharides, Bacterial / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits; D013211:Staphylococcus aureus / Q000032:analysis* / Q000276:immunology; D015164:Vaccines, Inactivated
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D006603:Hexuronic Acids; D007106:Immune Sera; D011135:Polysaccharides, Bacterial; D015164:Vaccines, Inactivated; C033657:compact-colony-forming active substance; C011399:2-amino-2-deoxygalacturonic acid; D000362:Agar; D005690:Galactose