[Use of peroxidase-labeled antigen of tick-borne encephalitis virus for analysis of specific immunoglobulin response and the evaluation of its diagnostic usefulness for detecting the etiological factor of human neurologic infections].
Peroxidase labelled virus of tick-borne encephalitis (k.z.m.) was used for humoral immune response analysis in humans. Dot-ELISA methods elaborated in a version of "catching antibodies" (cAb-ELISA) was compared in respect to its diagnostic usefulness to indirect immunoenzymatic method (i-ELISA) and to haemagglutination inhibition test (OZHA). High sensitivity and diagnostic value of both methods were confirmed. As compared to i-ELISA, cAb-ELISA was characterised by higher specificity of IgM antibody determination resulting from a lack of influence of rheumatoid factor and specific IgG on above mentioned determinations of antiviral IgM in cAb-ELISA. Moreover, parallel analysis by cAb-ELISA technique of serum and cerebro-spinal fluid permits to evaluate in a simple manner a synthesis of specific viral antibodies present in central nervous system.
Gut
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Zakład Wirusologii PZH w Warszawie.
Panfist
A
A
Jarzabek
Z
Z
D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology / Q000302:isolation & purification*; D004675:Encephalitis, Tick-Borne / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005188:False Negative Reactions; D006385:Hemagglutination Inhibition Tests / Q000379:methods; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007136:Immunoglobulins
Uzycie znakowanego peroksydaza antygenu wirusa kleszczowego zaplaenia mózgu do analizy swoistej odpowiedzi w klasach immunoglobulin i ocena diagnostycznej przydatności tej metody do ustalania etiologicznego czynnika neuroinfekcji człowieka.