Role of mixed-function oxidases and non-protein sulfhydryl content in [14C]-2-chloro-4-acetotoluidide binding to liver and kidney in starlings.
The compound 2-chloro-4-acetotoluidide (CAT) is highly toxic to many avian species, including the starling. In our earlier work, we demonstrated the covalent binding of radioactivity from [14C]-CAT to liver and kidneys of the starling. In the present study, the effects of inducers of mixed-function oxidase (MFO) and non-protein sulfhydryl (NPSH) depletor on the total and covalent binding of [14C]-CAT radioactivity to liver and kidney of the starling were examined. The total and covalently bound radioactivity from [14C]-CAT to liver and kidney were decreased significantly in the starling pretreated with the MFO inducer, 3-methylcholanthrene. However, pretreatment with phenobarbital, another inducer of MFO, had no effect. Pretreatment with the inhibitor of MFO, SKF 525-A, reduced the covalent binding of [14C]-CAT radioactivity to liver but not to kidney. There was no reduction in the NPSH content of liver or kidney following intravenous administration of CAT (3.5 mg kg-1). Reduction of NPSH levels in the liver or kidney following treatment with diethyl maleate caused a significant increase in the covalent binding of [14C]-CAT to kidney but not to liver.
Giri
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Department of Veterinary Pharmacology and Toxicology, School of Veterinary Medicine, University of California, Davis 95616.
Siegel
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DM
D000818:Animals; D001717:Birds / Q000378:metabolism*; D002250:Carbon Radioisotopes; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction; D005260:Female; D005978:Glutathione / Q000378:metabolism; D007668:Kidney / Q000201:enzymology / Q000378:metabolism*; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008298:Maleates / Q000494:pharmacology; D008748:Methylcholanthrene / Q000494:pharmacology; D006899:Mixed Function Oxygenases / Q000096:biosynthesis / Q000502:physiology*; D010634:Phenobarbital / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D014052:Toluidines / Q000378:metabolism* / Q000494:pharmacology
D002250:Carbon Radioisotopes; D008298:Maleates; D011725:Pyridines; D013438:Sulfhydryl Compounds; D014052:Toluidines; C000404:2-chloro-4-acetotoluidine; C013109:metapyrone; D008748:Methylcholanthrene; D006899:Mixed Function Oxygenases; C014476:diethyl maleate; D005978:Glutathione; D010634:Phenobarbital