title: Nucleotide regulation of vasoactive intestinal peptide binding to bovine thyroid plasma membranes.
abstract: The specific binding of vasoactive intestinal peptide (VIP) to bovine thyroid plasma membranes is inhibited by guanine nucleotides. Guanosine 5'-triphosphate (GTP) and the non-hydrolyzable GTP analogs guanosine 5'-beta,gamma-imidotriphosphate (Gpp(NH)p) and guanosine 5'-O-(3-thiotriphosphate) (GTP-gamma-S) inhibited markedly the binding of VIP to its receptors. This inhibition was higher with GTP than with Gpp(NH)p and GTP-gamma-S and was due to an increase of the rate of dissociation of peptide bound to membranes. Other nucleotides did not show any effect.
authors.affiliation: Department of Medical Biochemistry and Molecular Biology, School of Medicine, University of Sevilla, Spain.
authors.forename: J R
authors.initials: JR
authors.lastname: Calvo
authors.forename: M F
authors.initials: MF
authors.lastname: Morales
authors.forename: J C
authors.initials: JC
authors.lastname: Mesa
authors.forename: R
authors.initials: R
authors.lastname: Goberna
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology*; D013961:Thyroid Gland / Q000187:drug effects / Q000378:metabolism*; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
chemical_list: D014660:Vasoactive Intestinal Peptide; D006160:Guanosine Triphosphate