Multiple forms of cytoplasmic ER and PgR characterized by polyacrylamide gel electrophoresis.
The multiple forms of cytoplasmic ER and PgR were electrophoretically characterized by PAGE under non-denatured conditions and SDS PAGE under denatured conditions. The ordinary PAGE and SDS PAGE were modified in such a way as to reveal the authentic multiple forms of the hormone receptors studied. The experimental conditions were well-defined. The standard protein criteria for evaluation of molecular weights were established. The high polymers containing main activity can be revealed by 3% PAGE under non-denatured conditions and all the multiple forms can be revealed by 3% SDS PAGE under milder SDS coupling reaction. The multiple forms commonly occurring are monomer, dimer, tetramer and hexamer for both ER and PgR. For PgR, there are two monomer components A and B, dimer AB. Tetramer 2AB and hexamer 3 AB. Octamer 4 AB is observed sometimes.
Chen
Y M
YM
Southfield Oncology Institute, Inc., Southfield, Michigan 48034.
Vaughn
C B
CB
D000818:Animals; D003600:Cytosol / Q000737:chemistry / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004958:Estradiol / Q000378:metabolism; D005260:Female; D046911:Macromolecular Substances; D008970:Molecular Weight; D011372:Progestins / Q000378:metabolism; D011817:Rabbits; D011960:Receptors, Estrogen / Q000302:isolation & purification* / Q000378:metabolism; D011980:Receptors, Progesterone / Q000302:isolation & purification* / Q000378:metabolism; D014599:Uterus / Q000737:chemistry / Q000378:metabolism*
D046911:Macromolecular Substances; D011372:Progestins; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D004958:Estradiol