Sensitive method for measuring hydrolysis of enkephalins in plasma, using high-performance liquid chromatography with electrochemical detection.
This paper describes a simple and sensitive method for detection of [Leu]- and [Met]enkephalin and their N-terminal tyrosine-containing metabolic fragments (Tyr, Tyr-Gly, Tyr-Gly-Gly, and Tyr-Gly-Gly-Phe), using high-performance liquid chromatography with electrochemical detection. The method employs a carbon graphite working electrode with increased working electrode surface area (40 mm2). The procedures were applied to assay of the activities of enkephalin-degrading enzymes in whole plasma collected from rats, mice, and chicks.
Shibanoki
S
S
Department of Pharmacology, School of Medicine, Nihon University, Tokyo, Japan.
Weinberger
S B
SB
Ishikawa
K
K
Martinez
J L
JL
D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000097:blood*; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010450:Endopeptidases / Q000097:blood*; D004743:Enkephalin, Leucine / Q000097:blood; D004744:Enkephalin, Methionine / Q000097:blood; D004745:Enkephalins / Q000097:blood*; D006868:Hydrolysis; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000097:blood / Q000737:chemistry; D051381:Rats; D011919:Rats, Inbred Strains
D004745:Enkephalins; D010446:Peptide Fragments; D004744:Enkephalin, Methionine; D004743:Enkephalin, Leucine; D010450:Endopeptidases; D000626:Aminopeptidases; C020071:dipeptidyl carboxypeptidase