[Update on the role of vascular, plasmatic and cellular components in the regulation of the interaction of platelets with the subendothelium].
The interaction of platelets (Plts) with subendothelium (SE) or with extracellular matrices (ECM) generated by endothelial cells was studied under flow conditions. The role of: a) platelet membrane glycoproteins (GPS) or subunits; b) plasma adhesive proteins such as von Willebrand factor (VWF) and fibronectin (Fn); and c) structural proteins of the SE such as laminin (Lm). Specific antibodies or purified proteins were added to the perfusates. GPS. Monoclonal antibodies (MoAb) directed against distinct epitopes of the GPIIb-IIIa affected in different manner the deposition of platelets. MoAb EDU3 decreased (p less than 0.01) the surface of the vessel covered by platelets (% CS) and increased the presence of platelets in contact (% C) with the SE. MoAb C17 did not modify the % CS but altered the morphological characteristics of the aggregates. A monoclonal antibody against GPIIb, decreased the % CS (p less than 0.01) without influencing % C; VWF. A linear progression of the % CS was observed for VWF levels ranging from 0.1 to 0.6 U/mL. A plateau was reached for concentrations of vWF above 0.6 U/mL; Fn. Perfusions in which blood was reconstituted with Fn-depleted plasma showed the cooperation of this protein facilitating Plt-Plt interaction. No statistical differences were observed in the % CS when ECM were incubated with a MoAb against Fn (3E3); Lm. The % CS decreased statistically (p less than 0.01) when SE or ECM were incubated with an antibody to Lm. These results show the critical role of vascular, plasmatic and cellular components in the maintenance of and adequate platelet function.
Ordinas
A
A
Servicio de Hemoterapia y Hemostasia, Hospital Clínic i Provincial, Barcelona.
Escolar
G
G
Bastida
E
E
Castillo
R
R
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001792:Blood Platelets / Q000502:physiology*; D004730:Endothelium, Vascular / Q000502:physiology*; D005109:Extracellular Matrix / Q000378:metabolism; D005353:Fibronectins / Q000502:physiology; D007797:Laminin / Q000502:physiology; D010477:Perfusion; D010980:Platelet Membrane Glycoproteins / Q000276:immunology / Q000502:physiology; D011817:Rabbits; D014841:von Willebrand Factor / Q000502:physiology
D000911:Antibodies, Monoclonal; D005353:Fibronectins; D007797:Laminin; D010980:Platelet Membrane Glycoproteins; D014841:von Willebrand Factor
Actualización del papel de los componentes vasculares, plasmáticos y celulares en la regulación de la interacción de las plaquetas con el subendotelio.