Isolation of a crotoxin-like protein from the venom of a South American rattlesnake (Crotalus durissus collilineatus).
1. A crotoxin-like protein was isolated from the venom of a South American rattlesnake Crotalus durissus collilineatus. 2. Many of its properties are similar to those of crotoxin, including its non-covalent heterodimeric structure, electrophoretic mobility on SDS-PAGE, isoelectric focusing properties, toxicity in mice, immunological reactivity, multiple isoforms, phospholipase activity, peptide map, and instability on an anion-exchange column. 3. Results indicate that "collilineatus toxin" is strongly homologous with crotoxin, found in the venom of Crotalus durissus terrificus, and all other characterized rattlesnake neurotoxins.
Lennon
B W
BW
Department of Molecular Biology, University of Wyoming, Laramie 82071.
Kaiser
I I
II
D000818:Animals; D015153:Blotting, Western; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003435:Crotalid Venoms / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity; D003439:Crotoxin / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D007525:Isoelectric Focusing; D007928:Lethal Dose 50; D046911:Macromolecular Substances; D051379:Mice; D010449:Peptide Mapping; D010740:Phospholipases / Q000378:metabolism
D003435:Crotalid Venoms; D046911:Macromolecular Substances; D003439:Crotoxin; D010740:Phospholipases