title: Tissue architecture and glycosphingolipid content in human gliomas II-IV.
abstract: Contradictory results have been reported claiming either none, partial or almost complete correlation between the complexity of GSL compound profiles and the assumed glial tumor differentiation. Therefore an attempt was made to compare GSL patterns with both the general (final) tumor diagnosis and malignancy grade (WHO) as well as the regional evaluation of the histology and the grading in the tumor tissue pieces directly subjected to biochemical analysis. Regional and general (final) diagnosis did not always correspond, especially when more than one tissue sample of a given tumor was analyzed. Four GSL component patterns were identified by TLC: GSL-type I with gangliosides primarily of the simple Glac-family lacking sulfatide and the more complex Gtri- and Gtet-gangliosides, GSL-type II with ganglioside of the Glac- and Gtri-families, also without sulfatide, and GSL-type III, with more complex gangliosides of the Gtri- and Gtet-families in addition to Glac-gangliosides and sulfatide, similar to the normal brain pattern, and the pattern of normal brain. There was only insufficient correlation between these GSL-type patterns and final diagnoses. However, between regional diagnosis of astrocytoma II and GSL-type III on the one hand and glioblastoma multiforme IV and GSL-type I on the other hand, a coincidence of more than 85% was found. In only 50% the intermediate GSL-type II and glioma III were associated. There was no relation between GFAP or vimentin expression and histology or GSL-type both with regard to final and regional diagnoses. Regional astrocytoma architectures exhibiting GSL-type III were mostly fibrillary, whilst glioblastomas with GSL component pattern I had often a giant cell make up.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Abteilung Neuropathologie, Philipps-Universität, Marburg, FRG.
authors.forename: H D
authors.initials: HD
authors.lastname: Mennel
authors.forename: H
authors.initials: H
authors.lastname: Wiegandt
authors.forename: B L
authors.initials: BL
authors.lastname: Bauer
authors.forename: R
authors.initials: R
authors.lastname: Jennemann
authors.forename: A F
authors.initials: AF
authors.lastname: Rodden
authors.forename: W
authors.initials: W
authors.lastname: Schachenmayr
mesh_terms: D000943:Antigens, Differentiation / Q000032:analysis; D005910:Glioma / Q000737:chemistry / Q000175:diagnosis / Q000473:pathology*; D006028:Glycosphingolipids / Q000032:analysis*; D006801:Humans
chemical_list: D000943:Antigens, Differentiation; D006028:Glycosphingolipids