Anti-HIV effects of CD4-Pseudomonas exotoxin on human lymphocyte and monocyte/macrophage cell lines.
CD4(178)-PE40 is a recombinant protein consisting of the HIV envelope glycoprotein-binding region of human CD4 linked to active domains of Pseudomonas aeruginosa exotoxin A. The hybrid toxin selectively kills HIV-infected human T-cell lines and protects against HIV spread in mixtures of uninfected and infected cells. We now report that CD4(178)-PE40 also selectively kills chronically HIV-1-infected cells of monocyte/macrophage lineage. The results provide further support for therapeutic use of this hybrid toxin in the treatment of HIV-infected individuals.
Ashorn
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P
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Moss
B
B
Berger
E A
EA
D036002:ADP Ribose Transferases; D000163:Acquired Immunodeficiency Syndrome / Q000188:drug therapy; D000998:Antiviral Agents / Q000494:pharmacology*; D001427:Bacterial Toxins / Q000494:pharmacology*; D015704:CD4 Antigens; D002460:Cell Line; D005098:Exotoxins / Q000494:pharmacology*; D015497:HIV-1 / Q000187:drug effects; D006801:Humans; D000922:Immunotoxins / Q000494:pharmacology*; D007962:Leukocytes / Q000187:drug effects* / Q000382:microbiology; D008214:Lymphocytes / Q000187:drug effects / Q000382:microbiology; D008264:Macrophages / Q000187:drug effects* / Q000382:microbiology; D009000:Monocytes / Q000187:drug effects / Q000382:microbiology; D011994:Recombinant Proteins / Q000494:pharmacology*; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D000998:Antiviral Agents; D001427:Bacterial Toxins; D015704:CD4 Antigens; D005098:Exotoxins; D000922:Immunotoxins; D011994:Recombinant Proteins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A; C057689:CD4-Pseudomonas toxin