Cultivation of psoriatic keratinocytes and an attempt to isolate and detect a specific psoriatic antigen.
Keratinocytes from psoriatic lesion and healthy skin region obtained from the skin of a psoriatic patient were cultivated on lethally irradiated 3T3 cells. They multiplied twice as quickly as normal keratinocytes from healthy skin. Ten antigen fractions were prepared from cell suspensions of psoriatic keratinocytes and from the medium left after their cultivation. Later, they were evaluated in ELISA tests performed with the sera of psoriatic patients and healthy persons. The presence of specific antigens was not demonstrated.
Novotný
J
J
Institute of Sera and Vaccines, Praha.
Arenberger
P
P
Matousková
E
E
D000941:Antigens / Q000302:isolation & purification*; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015603:Keratinocytes / Q000473:pathology*; D011565:Psoriasis / Q000473:pathology*
D000941:Antigens