[Analysis of sera from two cattle herds suspected of brucellosis using a competition enzyme immunoassay with monoclonal antibodies].
The milk pools of two cattle herds in Lower Saxony were found to give positive results in an enzyme-linked immunosorbent assay (ELISA). The sera from these cattle were tested in competition enzyme immunoassays (cEIA) using two monoclonal antibodies. The results were compared to those obtained in the serum agglutination assay (SLA) and in an ELISA. The cEIAs detected more positive samples than the other tests. All sera that were positive in the SLA and in the ELISA were also positive in the cEIAs. Due to the higher sensitivity, these tests may be an alternative for the SLA.
Greiser-Wilke
I
I
Institut für Virologie Tierärztlichen Hochschule Hannover.
MacMillan
A P
AP
Moennig
V
V
D000372:Agglutination Tests; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood*; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D002002:Brucella / Q000276:immunology*; D002007:Brucellosis, Bovine / Q000175:diagnosis*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007124:Immunoenzyme Techniques; D008892:Milk / Q000276:immunology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal
Untersuchung von Seren zweier brucellose-verdächtiger Rinderbestände mittels eines Kompetitions-Enzymimmunoassays mit monoklonalen Antikörpern.