title: Effect of 3,4-dichloroaniline on the early life stages of the zebrafish (Brachydanio rerio): results of a comparative laboratory study.
abstract: An early life-stage (ELS) test was conducted with the zebrafish and 3,4-dichloroaniline (3,4-DCA) in eight laboratories. Based on the results from all eight laboratories, an LOEC of 200 micrograms 3,4-dichloroaniline/liter applies for the early life stages of the zebrafish. Effects observed were reduction of the survival rate and malformations. If the 100 micrograms/liter concentration additionally tested by one laboratory is included in the assessment, an LOEC of 100 micrograms 3,4-DCA/liter is obtained for the survival rate and increase in length. The NOEC is 20 micrograms/liter. The present results of a comparative laboratory study with the zebrafish show that a 42-day ELS test can be conducted with this species of fish, and affords meaningful results.
authors.affiliation: Institut für Zoologie, Johannes Gutenberg-Universität Mainz, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Nagel
authors.forename: H
authors.initials: H
authors.lastname: Bresch
authors.forename: N
authors.initials: N
authors.lastname: Caspers
authors.forename: P D
authors.initials: PD
authors.lastname: Hansen
authors.forename: M
authors.initials: M
authors.lastname: Markert
authors.forename: R
authors.initials: R
authors.lastname: Munk
authors.forename: N
authors.initials: N
authors.lastname: Scholz
authors.forename: B B
authors.initials: BB
authors.lastname: ter Höfte
mesh_terms: D000375:Aging / Q000502:physiology; D000814:Aniline Compounds / Q000032:analysis / Q000633:toxicity*; D000818:Animals; D005298:Fertility / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D015027:Zebrafish / Q000502:physiology*
chemical_list: D000814:Aniline Compounds; C014464:3,4-dichloroaniline