[Use of fermenter-dialysis culture for the cultivation of medically relevant microbes. III. Candida albicans].
For mass cultivation of Candida albicans (serotype A), a fermenter dialysis culture technique is described and compared with shaking culture and fermenter batch culture techniques. Important growth parameters such as yeast dry weight and viable cell counts demonstrate the advantage of the fermenter dialysis culture. Mannan, the major antigen from Candida albicans prepared by phenol-water extraction followed by gel chromatography was tested with the monoclonal IgM antibody H5.
Pfüller
R
R
Institut für Infektionen im Kindesalter des Klinikums Berlin-Buch.
Mehl
M
M
Starke
R
R
Hentschel
C
C
Grunow
R
R
Schmidt
G
G
von Baehr
R
R
D002176:Candida albicans / Q000254:growth & development*; D015169:Colony Count, Microbial; D003470:Culture Media; D005285:Fermentation
D003470:Culture Media
Einsatz der Fermenter-Dialysekultur zur Kultivierung medizinisch relevanter Erreger. III. Candida albicans.