title: [Immunohistochemical study of the surface membrane on a intra-ocular lens].
abstract: Surface membranes of intraocular lenses implanted in three rabbit, were studied immunohistochemically with anti-rabbit fibronectin serum and anti-rabbit whole serum antiserum. The specimens were embedded in an epoxy resin and cut at 1 micron. Before the immunostaining procedure, the sections were placed in a solution of saturated NaOH in absolute ethanol to remove the epoxy resin. Immunostaining procedure was performed by routine avidin-biotin-peroxidase technique. The surface membranes showed positive reactions against both anti-sera. These results indicated that the surface membrane of implanted intraocular lenses was composed of fibronectin with some other serum proteins.
authors.affiliation: Department of Pathology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sakamoto
authors.forename: T
authors.initials: T
authors.lastname: Ishibashi
authors.forename: S
authors.initials: S
authors.lastname: Sugai
authors.forename: Y
authors.initials: Y
authors.lastname: Ohnishi
authors.forename: M
authors.initials: M
authors.lastname: Fukushima
authors.forename: K
authors.initials: K
authors.lastname: Sueishi
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D007150:Immunohistochemistry; D007910:Lenses, Intraocular; D008566:Membranes / Q000378:metabolism; D011817:Rabbits
chemical_list: D001798:Blood Proteins; D005353:Fibronectins