[Sensitized NADH formation of single-stranded breaks in plasmid DNA upon the action of near UV-radiation].
It has been shown that NADH photosensitize in vitro single-strand breaks formation in double-strand plasmid DNA pBR 322 upon near-UV (320-400 nm) irradiation. The number of single-strand breaks depends both on UV light dose and sensitizer concentration. Addition of catalase and sodium benzoate strongly decreases the single-strand breaks formation. The results show an important role of hydrogen peroxide (H2O2) and hydroxyl radical (.OH) in inducing single-strand breaks in plasmid DNA irradiated by near-UV radiation in the presence of NADH.
Burchuladze
T G
TG
Sideris
E G
EG
Fraĭkin
G Ia
GIa
D001565:Benzoates / Q000494:pharmacology; D019817:Benzoic Acid; D002374:Catalase / Q000494:pharmacology; D004247:DNA / Q000187:drug effects / Q000528:radiation effects*; D004249:DNA Damage; D004587:Electrophoresis, Agar Gel; D006861:Hydrogen Peroxide / Q000633:toxicity; D009243:NAD / Q000096:biosynthesis*; D010957:Plasmids; D014466:Ultraviolet Rays
D001565:Benzoates; D009243:NAD; D019817:Benzoic Acid; D004247:DNA; D006861:Hydrogen Peroxide; D002374:Catalase
Sensibilizirovannoe NADH obrazovanie odnonitevykh razryvov v plazmidnoĭ DNK pri deĭstvii blizhnego UF-izlucheniia.