Effect of chronic halothane exposure on lipid peroxidation, osmotic fragility and morphology of rat erythrocytes.
Effect of chronic halothane exposure on hepatic and erythrocyte lipid peroxidation and erythrocyte osmotic fragility and morphology were determined in rats exposed to 0.4% halothane, 8 h per day for 40 days. Hepatic lipid peroxidation was increased in the halothane-treated group compared to controls. Lipid peroxidation was not increased by halothane exposure in erythrocytes without hydrogen peroxide, but after peroxide supplementation lipid peroxidation increased more in the erythrocytes of halothane-exposed rats than in control rats. We have observed significant morphological changes in erythrocytes from halothane-treated rats. In addition, erythrocytes of halothane-treated rats were more fragile in saline solutions compared to those of controls. Our results suggest that chronic halothane exposure is not only hepatotoxic but also affects erythrocyte membrane structure and stability.
Toker
K
K
Department of Anesthesiology and Reanimation, Faculty of Medicine, Marmara University, Istanbul, Turkey.
Ozer
N K
NK
Yalçin
A S
AS
Tüzüner
S
S
Göğüs
F Y
FY
Emerk
K
K
D000280:Administration, Inhalation; D000818:Animals; D004334:Drug Administration Schedule; D004910:Erythrocyte Membrane / Q000187:drug effects; D004912:Erythrocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006221:Halothane / Q000008:administration & dosage / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D009994:Osmolar Concentration; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D006221:Halothane