title: Outer membrane proteins of Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus studied by SDS-PAGE and immunoblotting.
abstract: This investigation characterized and compared outer membrane proteins (OMP) of the closely related Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus by means of SDS-PAGE patterns and reactions on immunoblots with rabbit antiserum against A. actinomycetemcomitans FDC Y4. Reactions with serum from a patient with Papillon Lefévre syndrome (PLS), from whom periodontal wild strains of A. actinomycetemcomitans had been isolated, were also studied. OMP were purified with selective solubilization from lyophilized cells of 10 wild and 4 reference strains of A. actinomycetemcomitans and 4 reference strains of H. aphrophilus. OMP profiles from wild and reference strains of A. actinomycetemcomitans were similar while those from A. actinomycetemcomitans and H. aphrophilus differed. The most prominent difference was absence of a heat modifiable protein in H. aphrophilus strains. Immunoblotting revealed strong common antigens in most strains, including a heat modifiable protein with mol wt 34 kDa, as well as a 29 kDa and a 16.5 kDa protein. Treatment with pronase and sodium periodate confirmed the protein nature of the major OMP antigens.
authors.affiliation: Department of Periodontology, University of Bergen, Norway.
authors.forename: A I
authors.initials: AI
authors.lastname: Bolstad
authors.forename: T
authors.initials: T
authors.lastname: Kristoffersen
authors.forename: I
authors.initials: I
authors.lastname: Olsen
authors.forename: H R
authors.initials: HR
authors.lastname: Preus
authors.forename: H B
authors.initials: HB
authors.lastname: Jesen
authors.forename: E N
authors.initials: EN
authors.lastname: Vasstrand
authors.forename: V
authors.initials: V
authors.lastname: Bakken
mesh_terms: D000188:Actinobacillus / Q000145:classification* / Q000276:immunology; D010520:Aggressive Periodontitis / Q000382:microbiology; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification; D002648:Child; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006190:Haemophilus / Q000145:classification* / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D010214:Papillon-Lefevre Disease / Q000382:microbiology
chemical_list: D001425:Bacterial Outer Membrane Proteins