Determination of amineptine and its main metabolite in plasma by high-performance liquid chromatography after solid-phase extraction.
Amineptine and its main metabolite were determined simultaneously in plasma by high-performance liquid chromatography using quinupramine as internal standard. The method comprised adsorption on Extrelut column from alkaline plasma, elution with diethyl ether-methylene chloride, evaporation in the presence of 0.01 M hydrochloric acid and injection of the acid solution onto a mu Bondapak C18 column, using acetonitrile-0.025 M potassium dihydrogenphosphate as mobile phase and ultraviolet detection at 210 nm. Average steady-state concentrations of the two compounds were determined in four patients under treatment regimen (two 100-mg doses of amineptine per day, at 8.00 and 12.00 h). The concentrations determined 20 h after the last dose were undetectable in all cases, whereas the concentrations determined 1 h after the second dose were found to be 780 +/- 96 ng ml-1 for amineptine and 690 +/- 137 ng ml-1 for its metabolite. The technique can also be applied to whole blood with, if necessary, identification on the basis of the ultraviolet spectrum obtained by photodiode-array detection.
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Laboratoire Interrégional de Police Scientifique, Marseille, France.
Spinazzola
J
J
Bresson
M
M
Conquy
T
T
Viala
A
A
D000327:Adsorption; D000928:Antidepressive Agents / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D003986:Dibenzocycloheptenes / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use; D004986:Ether; D006801:Humans; D008752:Methylene Chloride; D008832:Microchemistry; D008875:Middle Aged; D011786:Quality Control
D000928:Antidepressive Agents; D003986:Dibenzocycloheptenes; D004986:Ether; C011597:amineptin; D008752:Methylene Chloride