Immunoturbidimetric determination of pregnancy-specific beta 1-glycoprotein (SP-1).
We describe a simple and rapid immunoturbidimetric end-point assay for measuring pregnancy-specific beta 1-glycoprotein (SP-1) in human serum with the Kone Progress clinical chemistry analyser, using a commercially available antiserum. The absorbance was measured at 340 nm 5 min after addition of antiserum. The assay range was 3-250 mg/l. Over this range the CV for between-day precision was below 6%. Bilirubin up to 160 mumol/l, haemoglobin up to 2 g/l and lipaemia up to 320 mg/l did not interfere. The reference interval in maternal serum at the 15th week of gestation, was 13 to 46 mg/l with a median of 25 mg/l (n = 78) and that of the 16th week was 6 to 60 mg/l with a median of 28 mg/l (n = 78). Comparison of results obtained by two commercially available radio-immunoassays and by the present method shows acceptable correlation. Rapid and reproducible, the immunoturbidimetric method is suitable for use in routine determination of SP-1.
Turpeinen
U
U
Department of Obstetrics and Gynaecology, Helsinki University Central Hospital, Finland.
Stenman
U H
UH
D005260:Female; D006801:Humans; D009391:Nephelometry and Turbidimetry; D011247:Pregnancy; D011268:Pregnancy-Specific beta 1-Glycoproteins / Q000032:analysis* / Q000276:immunology; D011863:Radioimmunoassay
D011268:Pregnancy-Specific beta 1-Glycoproteins