Interaction of fluconazole and human phagocytic cells. Uptake of the antifungal agent and its effects on the survival of ingested fungi in phagocytes.
3H-labelled fluconazole (CAS 86386-73-4) very rapidly penetrated into polymorphonuclear leucocytes (PMNLs) and macrophages (monocytes) isolated from volunteers. The concentration of the antimycotic in the PMNLs was about 28% and in the macrophages even 63% above that in the extracellular medium. At the concentrations examined (5, 10 and 20 micrograms/ml) fluconazole damaged cells of Candida albicans which had been phagocytized by PMNLs or macrophages. There is an evident synergism between therapeutically attainable concentrations of fluconazole and phagocytic cells and this militates against the intracellular survival of the fungus.
Wildfeuer
A
A
Department of Pathology, Faculty of Theoretical Medicine, University of Ulm/Donau, Fed. Rep. of Germany.
Laufen
H
H
Haferkamp
O
O
D002176:Candida albicans / Q000187:drug effects; D015725:Fluconazole / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008264:Macrophages / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects; D010586:Phagocytes / Q000187:drug effects* / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects
D015725:Fluconazole