[Features of a protein component of the endotoxin from Yersinia pseudotuberculosis].
The protein moiety of endotoxin from Yersinia pseudotuberculosis was found to consist of two polypeptides with apparent molecular masses 40 and 14.5 kDa (4:1 w/w). The major protein (40 kDa) was isolated from the endotoxin pretreated with sodium deoxy cholate by gel chromatography on the Sephadex G-200 column. Comparative study of this protein and oligomeric form of porin from the outer membrane of Y. pseudotuberculosis using SDS--PAGE, velocity sedimentation, lipid bilayer experiments, chemical and serological analyses revealed their identity. The deoxycholate treatment of the endotoxin does not affect complexes of the major protein and LPS.
Solov'eva
T F
TF
Bakholdina
S I
SI
Ermak
I M
IM
Khomenko
V A
VA
Fedoreeva
L I
LI
Novikova
O D
OD
Frolova
G M
GM
Likhatskaia
G N
GN
Ovodov
Iu S
IuS
D000596:Amino Acids / Q000032:analysis; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000737:chemistry*; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000737:chemistry; D015011:Yersinia pseudotuberculosis / Q000378:metabolism*
D000596:Amino Acids; D001425:Bacterial Outer Membrane Proteins; D004731:Endotoxins; D008070:Lipopolysaccharides
Kharakteristika belkovogo komponenta éndotoksina iz Yersinia pseudotuberculosis.