title: [Study of the structure of the photosynthetic reaction center of the green thermophilic bacteria Chloroflexus aurantiacus].
abstract: Analysis of the Chloroflexus aurantiacus reaction centre (RC) using both protein and recombinant DNA techniques resulted in determination of its polypeptide composition and the primary structures of its two subunits. A model of the polypeptide chains' folding in the membrane is suggested based on: i) homology between L- and M-subunits of Chloroflexus aurantiacus RC and their counterparts in purple bacteria; ii) comparison of their hydropathy plots, and iii) data on the tertiary structures of purple bacteria RCs. The role of a number of functionally important amino acid residues in the RC electron transport activity is discussed. Limited proteolysis of the RC under non-denaturing conditions was used to determine the contribution of the N-terminal regions to its thermal stability.
authors.forename: M A
authors.initials: MA
authors.lastname: Kutuzov
authors.forename: B E
authors.initials: BE
authors.lastname: Shmukler
authors.forename: A A
authors.initials: AA
authors.lastname: Zargarov
authors.forename: I N
authors.initials: IN
authors.lastname: Telezhinskaia
authors.forename: N B
authors.initials: NB
authors.lastname: Levina
authors.forename: A S
authors.initials: AS
authors.lastname: Zolotarev
authors.forename: N G
authors.initials: NG
authors.lastname: Abdulaev
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000235:genetics*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D045322:Photosynthetic Reaction Center Complex Proteins
vernacular_title: Izuchenie structury fotosinteticheskogo reaktsionnogo tsentra zelenoĭ termofil'noĭ bakterii Chloroflexus aurantlacus.