Distribution of mouse interferon-beta in normal and brain tumour-bearing mice.
The distribution of 125I-labelled recombinant mouse interferon-beta (rMuIFN-beta) in normal and glioma (203 glioma) bearing mice was studied by radioassay and macro-autoradiography at 15 and 30 min after a single intravenous injection. The level of rMuIFN-beta in the spleen was about 20-fold higher than in serum. Concentrations higher than the serum level was detected in the lung, liver and kidney. The concentration of rMuIFN-beta in the brain was 8% of the serum level and the concentration in the glioma 30 min after administration was about 10-fold higher than in normal mouse brain. Macro-autoradiographic study demonstrated a wide distribution range and selective uptake in glioma tissue. Furthermore, we found that mouse gliomas were sensitive to mouse IFN-beta. Our findings demonstrate that in the mouse glioma model, intravenously administered interferon reaches the tumour.
Mihara
Y
Y
Department of Neurosurgery, Kumamoto University Medical School, Japan.
Kuratsu
J
J
Takaki
S
S
Hori
K
K
Nagai
E
E
Satoh
Y
Y
Minowa
N
N
Ushio
Y
Y
D000818:Animals; D001345:Autoradiography; D001932:Brain Neoplasms / Q000276:immunology* / Q000628:therapy; D002460:Cell Line; D005910:Glioma / Q000276:immunology* / Q000628:therapy; D007262:Infusions, Intravenous; D007370:Interferon Type I / Q000008:administration & dosage / Q000493:pharmacokinetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D011994:Recombinant Proteins
D007370:Interferon Type I; D011994:Recombinant Proteins