[Phenotyping of apolipoprotein E: immunoblotting after isoelectric focusing in immobilized pH gradients].
Apolipoprotein E is a major ligand for cellular lipoprotein receptors and plays an important role in the catabolism of triglyceride-rich lipoproteins. The polymorphism of apo E is associated with familial dysbetalipoproteinemia, LDL-cholesterol, and cardiovascular risk. In this report a method for the analysis of apo E isoforms in immobilized pH gradients is presented that does not require isolation apo E containing lipoproteins by ultracentrifugation.
März
W
W
Gustav Embden-Zentrum der Biologischen Chemie, J.W. Goethe-Universität, Frankfurt am Main.
Cezanne
S
S
Gross
W
W
D001057:Apolipoproteins E / Q000235:genetics*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015151:Immunoblotting; D007525:Isoelectric Focusing; D010641:Phenotype; D011110:Polymorphism, Genetic / Q000235:genetics*
D001057:Apolipoproteins E
Phänotypisierung des Apolipoprotein E: Immunoblotting nach isoelektrischer Fokussierung in immobilisierten pH Gradienten.