title: Genetic control of capsid length in bacteriophage T4. VII. A model of length regulation based on DNA size.
abstract: Four models for head length regulation in bacteriophage T4 are described and discussed. Several length mutants in the major capsid protein gene (23) were studied by sucrose gradient analysis, rotating gel analysis of DNA length, and by mixed infection gene dosage experiments with T4 amber mutants in gene 24. The results show that head length variation is quantized and highly specific, in that certain amino acid changes in gp23 results in reproducible and well-defined head length phenotypes. These data are presented as being most consistent with a vernier-type of head length control mechanism.
authors.affiliation: Microbiology Department, University of California, Los Angeles 90024-1489.
authors.forename: T
authors.initials: T
authors.lastname: Lane
authors.forename: F
authors.initials: F
authors.lastname: Eiserling
mesh_terms: D002213:Capsid / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient; D004279:DNA, Viral / Q000235:genetics* / Q000648:ultrastructure; D004926:Escherichia coli / Q000235:genetics* / Q000648:ultrastructure; D005613:Freeze Etching; D005814:Genes, Viral; D007700:Kinetics; D008854:Microscopy, Electron; D008961:Models, Structural; D016296:Mutagenesis; D011487:Protein Conformation; D013604:T-Phages / Q000235:genetics* / Q000648:ultrastructure; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D015678:Viral Structural Proteins