[The clinical significance of flow cytometric DNA measurement of paraffin specimen in bladder tumor].
A retrospective study of flow cytometric measurements on paraffin embedded tumor specimens from 188 patients with bladder tumor was made and the results were analyzed in combination with the morphological changes of bladder tumors. It was found that the DNA ploidy pattern, degree of infiltration and the multiplicity of bladder tumor were closely related with tumor recurrence, among which the DNA ploidy pattern was most significant. In the aneuploid bladder tumors the recurrent rate and mean annual recurrent frequency were 76.7% and 1.46, and those in the diploid bladder tumors were 18.7% and 0.33 respectively. Aneuploidy was the most indicative parameter in the recurrence of bladder tumor. In addition, according to the DNA ploidy pattern and DNA index, the aneuploid tumors in our group were divided into 4 types, namely, tetraploid tumors, non-euploid tumors with DNA index less than or equal to 1.5, non-euploid tumors with DNA index greater than 1.5 and two-aneuploid tumors. The results showed that the recurrent rate of tetraploid tumor was relatively low and it became higher and higher in the following order, e.g., non-euploid tumors with DNA index less than or equal to 1.5, non-euploid tumors with DNA index greater than 1.5 and two-aneuploid tumors, indicating that there were different biological behaviors in tumors with different ploidy patterns. Finally, the relationship between DNA ploidy pattern and tumor metastasis was also discussed.
Wang
Z
Z
Zhongshan Hospital, Shanghai Medical University.
D000782:Aneuploidy; D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D012189:Retrospective Studies; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology
D004273:DNA, Neoplasm