title: Glycosyltransferase activities in chondrocytes from osteoarthritic and normal human articular cartilage.
abstract: Six glycosyltransferases (mannosyl-, glucosyl-, N-acetyl-glucosaminyl-, galactosyl-, sialyl- and fucosyltransferases) are studied and characterized for their optimal conditions and their relations with interfering reactions (glycosyl-nucleotide pyrophosphatases, glycosidases and proteinases) in chondrocytes from osteoarthritic and normal human articular cartilage. Osteoarthritis induces increased activities for five glycosyl-transferases. The observed modifications are not explained by alterations in physico-chemical parameters of the enzymes or by intervention of glycosyl-nucleotide pyrophosphatases, glycosidases or proteolytic enzymes.
authors.affiliation: Department of General and Medical Biochemistry, INSERM-CNRS U189. Lyon-Sud Medical School, Oullins, France.
authors.forename: M
authors.initials: M
authors.lastname: Richard
authors.forename: E
authors.initials: E
authors.lastname: Vignon
authors.forename: M J
authors.initials: MJ
authors.lastname: Peschard
authors.forename: P
authors.initials: P
authors.lastname: Broquet
authors.forename: J P
authors.initials: JP
authors.lastname: Carret
authors.forename: P
authors.initials: P
authors.lastname: Louisot
mesh_terms: D002358:Cartilage, Articular / Q000166:cytology / Q000201:enzymology*; D006031:Glycosylation; D006602:Hexosyltransferases / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D010003:Osteoarthritis / Q000201:enzymology*; D012016:Reference Values
chemical_list: D006602:Hexosyltransferases; C018660:asparagine-N-glycosyltransferase