Reactivity of monoclonal anti-human skin basal cell antibody to cultured keratinocytes in serum-free medium.
The reactivity of the monoclonal anti-human skin basal cell antibody (3B4-6) to cultured keratinocytes was examined by immunofluorescence techniques in order to verify the change in distribution of basal cell antigens during the in vitro differentiation of keratinocytes cultured in serum-free medium. The basal cells' antigen(s) determinants recognized by 3B4-6 were present in the cytoplasm of the cells at low concentration of Ca2+ (0.03 mM). High concentrations of Ca2+ (0.1 mM approximately 0.3 mM) induced the reactivity on the cell surface. This result was confirmed by flow cytometry analysis. The staining pattern was different from that of anti-involucrin antibody in 0.3 mM Ca2+ culture medium. This MoAb might be a useful tool for monitoring the degree of differentiation of cultured keratinocytes.
Furukawa
F
F
Department of Dermatology, Faculty of Medicine, Kyoto University, Japan.
Norris
D A
DA
Kashihara-Sawami
M
M
Lyons
M B
MB
Ueda
M
M
Imamura
S
S
D000911:Antibodies, Monoclonal / Q000276:immunology*; D002118:Calcium / Q000032:analysis / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D011498:Protein Precursors / Q000276:immunology; D012867:Skin / Q000166:cytology*
D000911:Antibodies, Monoclonal; D003470:Culture Media; D011498:Protein Precursors; C032253:involucrin; D002118:Calcium