title: DNA diagnosis of hydatidiform mole using the polymerase chain reaction.
abstract: We have used the polymerase chain reaction (PCR) technique for the diagnosis of hydatidiform mole, a trophoblastic disease. For this, we targeted the hypervariable 3' flanking region of the APOB gene (APOB/VNTR) because of its high heterozygosity index (0.61) in the Japanese population. We examined seven clinical cases which were tentatively diagnosed as hydatidiform moles. Five of these revealed DNA segments unique to the paternal APOB allele, allowing us to diagnose a complete mole. The PCR technique for targeting the APOB/VNTR appears useful for early diagnosis of hydatidiform mole.
authors.affiliation: Department of Molecular Biology, Keio University School of Medicine, Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Fukuyama
authors.forename: M
authors.initials: M
authors.lastname: Takata
authors.forename: J
authors.initials: J
authors.lastname: Kudoh
authors.forename: K
authors.initials: K
authors.lastname: Sakai
authors.forename: S
authors.initials: S
authors.lastname: Tamura
authors.forename: N
authors.initials: N
authors.lastname: Shimizu
mesh_terms: D000483:Alleles; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D006828:Hydatidiform Mole / Q000175:diagnosis* / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D014594:Uterine Neoplasms / Q000175:diagnosis* / Q000235:genetics