title: Oxidation-reduction potentials of cytochromes in Ascaris muscle mitochondria: high-redox-potential cytochrome b558 in complex II (succinate-ubiquinone reductase).
abstract: Oxidation-reduction midpoint potentials (Ems) were determined at pH 7.0 for cytochromes in the anaerobic respiratory chain of Ascaris mitochondria by redox titration techniques. Cytochrome b558, which is associated with complex II that functions as fumarate reductase in the terminal step of the respiratory chain, was shown to have an Em of -34 mV in the isolated complex II and -54 mV in mitochondria. These values are much higher than the value of Ascaris cytochrome b558. In contrast, Ems of cytochromes C + C1 and cytochrome b559.5 were determined in situ to be 235 mV and 78 mV, respectively, which are comparable to those of their mammalian counterparts.
authors.affiliation: Department of Parasitology, Juntendo University, School of Medicine, Tokyo Japan.
authors.forename: S
authors.initials: S
authors.lastname: Takamiya
authors.forename: K
authors.initials: K
authors.lastname: Kita
authors.forename: K
authors.initials: K
authors.lastname: Matsuura
authors.forename: R
authors.initials: R
authors.lastname: Furushima
authors.forename: H
authors.initials: H
authors.lastname: Oya
mesh_terms: D000818:Animals; D001200:Ascaris / Q000201:enzymology*; D002417:Cattle; D003573:Cytochrome b Group / Q000378:metabolism*; D042963:Electron Transport Complex II; D008931:Mitochondria, Muscle / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000378:metabolism*; D019255:NADPH Oxidases; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000378:metabolism*
chemical_list: D003573:Cytochrome b Group; D009097:Multienzyme Complexes; C036888:cytochrome b558; D010088:Oxidoreductases; D042963:Electron Transport Complex II; D013385:Succinate Dehydrogenase; D019255:NADPH Oxidases