The photocycle of bacteriorhodopsin immobilized in poly(vinyl alcohol) film.
The kinetics of photoelectric and optical signals were measured on samples containing oriented purple membranes immobilized in a poly(vinyl alcohol) film and on purple membranes introduced into a PVA-H2O mixture. The bacteriorhodopsin photocycle in the PVA-H2O mixture was complete. The only observed changes were the slowing down of the optical and electrical signals in relation to the M412-O640 and O640-bRall-trans steps. In the PVA film the O640 intermediate disappeared and a negative photoelectric signal appeared.
Bryl
K
K
Department of Physics and Biophysics, University of Agriculture and Technology, Olsztyn, Poland.
Varo
G
G
Drabent
R
R
D001436:Bacteriorhodopsins / Q000737:chemistry*; D004553:Electric Conductivity; D006217:Halobacterium / Q000032:analysis*; D007700:Kinetics; D008027:Light; D046911:Macromolecular Substances; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D013053:Spectrophotometry; D014867:Water
D046911:Macromolecular Substances; D014867:Water; D001436:Bacteriorhodopsins; D011142:Polyvinyl Alcohol