The hemimethylated replication origin of Escherichia coli can be initiated in vitro.
Unmethylated, fully methylated, and hemimethylated oriC-containing plasmids were assayed as substrates for DNA replication in vitro by using a system reconstituted with pure proteins. In contrast to the in vivo situation, all three substrates were initiated efficiently; the fully methylated plasmid was about twice as active as the other two.
Boye
E
E
Department of Biophysics, Norwegian Radium Hospital, Oslo.
D001426:Bacterial Proteins / Q000378:metabolism; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics*; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000235:genetics*; D007700:Kinetics; D008745:Methylation; D010957:Plasmids; D015183:Restriction Mapping
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria