title: A genetic mosaic screen of essential zygotic genes in Caenorhabditis elegans.
abstract: We have devised a simple genetic mosaic screen, which circumvents the difficulties posed by phenotypic analysis of early lethal mutants, to analyze essential zygotic genes in Caenorhabditis elegans. The screen attempts to distinguish genes involved in cell type and/or lineage specific processes such as determination, differentiation or morphogenesis from genes involved in general processes such as intermediary metabolism by using the pattern of gene function to classify genes: genes required in one or a subset of early blastomeres may have specific functions, whereas genes required in all early blastomeres may have general functions. We found that 12 of 17 genes examined function in specific early blastomeres, suggesting that many zygotic genes contribute to specific early processes. We discuss the advantages and limitations of this screen, which is applicable to other regions of the C. elegans genome.
authors.affiliation: Department of Molecular Biology, Princeton University, New Jersey 08544.
authors.forename: E A
authors.initials: EA
authors.lastname: Bucher
authors.forename: I
authors.initials: I
authors.lastname: Greenwald
mesh_terms: D000483:Alleles; D000818:Animals; D002107:Caenorhabditis / Q000166:cytology / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D002455:Cell Division; D005804:Genes, Lethal; D005816:Genetic Complementation Test; D005821:Genetic Techniques; D009030:Mosaicism / Q000235:genetics*; D005810:Multigene Family; D016296:Mutagenesis; D010641:Phenotype; D015053:Zygote