The timing of synthesis of proteins required for mitotic spindle and phragmoplast in partially synchronized root meristems of Vicia faba L.
After cycloheximide treatment (1 h, 2.5 micrograms/ml) protein synthesis was decreased by 70% and was partially restored after 7 h of postincubation (still 20% decrease). In partially synchronized root meristems of Vicia faba L. treated with cycloheximide at middle G2, a strong decrease of the mitotic index was observed. Exposure to the drug at late G2 did not modify the mitotic index; the changes in the phase indices suggested that the course of mitosis was blocked at prophase-metaphase/anaphase-telophase transitions. The use of indirect immunocytochemical staining of tubulin (second antibody labeled with peroxidase) made it possible to show a decreased number of cells with preprophase bands in cycloheximide-treated meristems and the mitotic spindles and phragmoplasts containing a reduced number of shortened bands of microtubules. As a result of these structural and functional disturbances, binucleate cells and polyploid nuclei were observed.
Olszewska
M J
MJ
Department of Plant Cytology and Cytochemistry, University of Lodz, Poland.
Marciniak
K
K
Kuran
H
H
D003513:Cycloheximide / Q000494:pharmacology; D007150:Immunohistochemistry; D007399:Interphase; D008938:Mitosis; D008940:Mitotic Index; D010940:Plant Proteins / Q000096:biosynthesis*; D010944:Plants / Q000378:metabolism* / Q000648:ultrastructure; D008941:Spindle Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D014404:Tubulin / Q000378:metabolism
D010940:Plant Proteins; D014404:Tubulin; D003513:Cycloheximide