title: Bronchial inflammation and hyperreactivity after anaphylactic shock in guinea pigs actively sensitized by systemic or aerosol route.
abstract: Hyperreactivity and bronchial inflammation resulting from active anaphylactic shock induced by aerosol have been studied in guinea pigs after sensitization by intramuscular injection of large-dose ovalbumin or aerosol ovalbumin. When animals were sensitized by i.m. injection of 30 mg/kg ovalbumin, hyperreactivity to inhalation of histamine was obtained 1-3 h after shock. In bronchoalveolar lavage (BAL) fluid an increase in the number of eosinophils (6-48 h after shock) and neutrophils (6-24 h) was observed. When guinea pigs were sensitized by aerosol route, the hyperreactivity to histamine inhalation appeared 1-6 h after shock. In BAL fluid the number of mononuclear cells dropped (1-3 h) and then increased (24-48 h); the number of neutrophils (6-48 h) and eosinophils (24-48 h) increased. The results observed during these two types of sensitization were compared to those obtained after sensitization by injection of a large dose of ovalbumin mixed with Freund's complete adjuvant.
authors.affiliation: Centre de Recherche Pierre Fabre, Castres, France.
authors.forename: J P
authors.initials: JP
authors.lastname: Tarayre
authors.forename: M
authors.initials: M
authors.lastname: Aliaga
authors.forename: M
authors.initials: M
authors.lastname: Barbara
authors.forename: N
authors.initials: N
authors.lastname: Malfetes
authors.forename: S
authors.initials: S
authors.lastname: Vieu
authors.forename: J
authors.initials: J
authors.lastname: Tisné-Versailles
mesh_terms: D000336:Aerosols; D000707:Anaphylaxis / Q000150:complications / Q000276:immunology*; D000818:Animals; D001991:Bronchitis / Q000209:etiology / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D004804:Eosinophils / Q000187:drug effects; D006168:Guinea Pigs; D006967:Hypersensitivity / Q000276:immunology*; D007273:Injections, Intramuscular; D008297:Male; D009504:Neutrophils / Q000187:drug effects; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000336:Aerosols; D010047:Ovalbumin