[Human sex identification in dried bloodstains by analysis of amplified DNA sequences].
This paper describes a technique of DNA amplification in vitro and its application on sex identification of human dried bloodstains. Dried bloodstains (8 males and 8 females) were prepared on filter paper, and then DNA was extracted. Target DNA sequences (Y-3.4kb repeat and Alu-repeat) were amplified by polymerase chain reaction (PCR) with two pairs of primers (Y1.1, Y1.2 and Alu9.1, Alu9.2). Amplification products were obtained in 8 male's bloodstains with the primers Y1.1 and Y1.2, but not in 8 female's bloodstains; and in 4 male's and 4 female's bloodstains with Alu9.1 and Alu9.2.
Sun
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Wu
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D001483:Base Sequence; D001801:Blood Stains; D004247:DNA / Q000235:genetics*; D005260:Female; D005784:Gene Amplification; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012732:Sex Determination Analysis
D004247:DNA