title: Release of tumor necrosis factor alpha by human peritoneal macrophages in response to toxic shock syndrome toxin-1.
abstract: We examined the release in vitro of tumor necrosis factor-alpha (TNF-alpha) by peritoneal macrophages and peripheral blood monocytes following incubation with toxic shock syndrome toxin-1 (TSST-1). We obtained peritoneal macrophages from 22 women at laparoscopy and peripheral blood monocytes from four healthy women during both the midfollicular and midluteal phases of the menstrual cycle. The samples were incubated for 24 hours at 37 C with 10(-2)-10(4) ng/mL of TSST-1 or 10(4) ng/mL of bacterial endotoxin. Tumor necrosis factor-alpha activity was determined with a bioassay using an actinomycin D-sensitized WEHI-164 murine fibrosarcoma cell line. Twenty-four-hour incubation with TSST-1 resulted in a dose-dependent release of TNF-alpha by both peritoneal macrophages (maximal response 554 +/- 97 U of activity) and peripheral blood monocytes (maximal response 478 +/- 81 U of activity). We observed enhanced TNF-alpha release by peritoneal macrophages from women with endometriosis, compared with those without endometriosis, at a concentration of 10(4) ng/mL of TSST-1 (704 +/- 134 versus 354 +/- 103 U of activity; P less than .05). These data support the theory that the metabolic and physiologic derangements of perimenstrual toxic shock syndrome may be partially mediated by TNF-alpha released by peritoneal macrophages as a result of exposure to TSST-1.
authors.affiliation: Department of Obstetrics and Gynecology, University of California, Los Angeles.
authors.forename: R P
authors.initials: RP
authors.lastname: Buyalos
authors.forename: E M
authors.initials: EM
authors.lastname: Rutanen
authors.forename: E
authors.initials: E
authors.lastname: Tsui
authors.forename: J
authors.initials: J
authors.lastname: Halme
mesh_terms: D000328:Adult; D001202:Ascitic Fluid / Q000166:cytology; D001427:Bacterial Toxins; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004715:Endometriosis / Q000097:blood; D004768:Enterotoxins / Q000494:pharmacology / Q000502:physiology*; D005260:Female; D005833:Genital Neoplasms, Female / Q000097:blood; D006801:Humans; D066298:In Vitro Techniques; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D008597:Menstrual Cycle / Q000502:physiology; D009000:Monocytes / Q000378:metabolism; D013211:Staphylococcus aureus; D018089:Superantigens; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D018089:Superantigens; D014409:Tumor Necrosis Factor-alpha; C030107:enterotoxin F, Staphylococcal