title: Capillary electrophoresis of amino sugars with laser-induced fluorescence detection.
abstract: 3-(4-Carboxybenzoyl)-2-quinolinecarboxaldehyde has been utilized as a precolumn derivatization agent for various amino sugars. Constituents of various biological mixtures can be converted to highly fluorescent isoindole derivatives, separated by high-performance capillary electrophoresis and determined at attomole (10(-18) mol) levels by a laser-induced fluorescence detector. This method has been applied to the analysis of monosaccharides and acid-hydrolyzed polysaccharides. Carbohydrate moieties derived from a glycoprotein were also tagged and determined.
authors.affiliation: Department of Chemistry, Indiana University, Bloomington 47405.
authors.forename: J P
authors.initials: JP
authors.lastname: Liu
authors.forename: O
authors.initials: O
authors.lastname: Shirota
authors.forename: M
authors.initials: M
authors.lastname: Novotny
mesh_terms: D000606:Amino Sugars / Q000032:analysis* / Q000737:chemistry; D004586:Electrophoresis / Q000379:methods*; D006868:Hydrolysis; D007834:Lasers; D013050:Spectrometry, Fluorescence
chemical_list: D000606:Amino Sugars