Restoration of energy metabolism and resolution of oedema following profound ischaemia.
Cerebral ischaemia was produced in 2 groups of gerbils by occlusion of the common carotid arteries for 30 minutes, resulting in cerebral oedema. In group 1 cerebral oedema was measured by specific gravity microgravimetry, and in group 2 brain metabolism and blood flow were measured by 31P and 1H NMR spectroscopy and hydrogen clearance respectively. In group 1 the brain water content did not return to control levels by 180 minutes of reperfusion. Energy metabolism, determined by 31P NMR spectroscopy returned to control by 12 minutes, intracellular pH (pHi) by 20 minutes, and lactate, determined by 1H NMR spectroscopy, by 50 minutes. There was a lag of about 10 minutes before lactate began to be cleared from the brain. We suggest that while pHi is low, Na+/H+ exchange will negate the Na+ extrusion driven by the Na+/K+ ATPase. When pHi approaches normal there will be a net extrusion of Na+, taking osmotic water with it, and presumably with passive washout of lactate. This may be the cause of the initial delay in lactate clearance.
Allen
K L
KL
Institute of Neurology, London, U.K.
Busza
A L
AL
Proctor
E
E
Williams
S R
SR
Van Bruggen
N
N
Gadian
D G
DG
Crockard
H A
HA
D000818:Animals; D001834:Body Water / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D001929:Brain Edema / Q000175:diagnosis / Q000209:etiology* / Q000378:metabolism; D002545:Brain Ischemia / Q000150:complications*; D004734:Energy Metabolism; D005849:Gerbillinae; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013046:Specific Gravity