Inhibitors of membrane transmethylation reactions prevent the lymphocyte chemokinetic response.
The methylation of membrane phospholipids has been shown to occur following receptor-mediated activation of leukocytes. The present studies show that the human lymphocyte response to two positive chemokinetic signals (bradykinin and lymphocyte chemoattractant factor) can be interrupted by inhibitors of S-adenosyl-L-methionine-mediated transmethylation reactions. The chemokinetic response to the nonphysiologic stimulant colchicine is not affected. We speculate that phospholipid methylation accompanies receptor-mediated lymphocyte migration, and may facilitate activation of second messenger systems.
McFadden
R G
RG
Department of Medicine, Lawson Research Institute of St. Joseph's Health Centre, London, Ontario, Canada.
Fraher
L J
LJ
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D058892:Adenosine Deaminase Inhibitors; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D003078:Colchicine / Q000494:pharmacology; D006710:Homocysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D019410:Interleukin-16; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects; D008222:Lymphokines / Q000494:pharmacology; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism*; D008745:Methylation / Q000187:drug effects; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012436:S-Adenosylmethionine / Q000037:antagonists & inhibitors* / Q000378:metabolism; D015290:Second Messenger Systems
D058892:Adenosine Deaminase Inhibitors; D019410:Interleukin-16; D008222:Lymphokines; D008563:Membrane Lipids; D010743:Phospholipids; D006710:Homocysteine; C010192:9-(2-hydroxy-3-nonyl)adenine; D012436:S-Adenosylmethionine; C007957:homocysteine thiolactone; D000225:Adenine; D001920:Bradykinin; D003078:Colchicine