title: Granulocyte proteases do not process endothelial cell-derived unusually large von Willebrand factor multimers to plasma vWF in vivo.
abstract: The unusually large von Willebrand factor (ULvWF) multimers present within endothelial cells and platelets are larger than the vWF multimers normally found in adult human plasma. Furthermore, ULvWF multimers are cleared rapidly from the circulation if they are released by intense endothelial cell stimulation. The mechanisms by which the ULvWF multimers are processed to large plasma vWF multimers are not known. It has been demonstrated that granulocyte proteases are capable of decreasing vWF multimer size in vitro, and that some patients with myeloproliferative syndromes have a relative absence of large plasma vWF multimers in sodium citrate-anticoagulated plasma samples. In order to assess the influence of granulocyte proteases on vWF multimer size, we evaluated the vWF multimeric patterns in 94 plasma samples from 60 patients with neutrophil counts that were either considerably elevated or extremely reduced. In 83 of 94 plasma samples, the vWF multimeric patterns were normal. No patients with very low neutrophil counts had ULvWF multimers present. These observations suggest that granulocyte proteases are not likely to be involved in vivo in the processing of ULvWF multimers from endothelial cells to the smaller vWF forms in circulation.
authors.affiliation: Baylor College of Medicine, Methodist Hospital, Houston, Texas 77030.
authors.forename: M D
authors.initials: MD
authors.lastname: Phillips
authors.forename: C
authors.initials: C
authors.lastname: Vu
authors.forename: L
authors.initials: L
authors.lastname: Nolasco
authors.forename: J L
authors.initials: JL
authors.lastname: Moake
mesh_terms: D010450:Endopeptidases / Q000276:immunology*; D004730:Endothelium, Vascular / Q000378:metabolism*; D006098:Granulocytes / Q000201:enzymology*; D006801:Humans; D007958:Leukocyte Count; D009504:Neutrophils / Q000473:pathology; D014841:von Willebrand Factor / Q000737:chemistry / Q000378:metabolism*
chemical_list: D014841:von Willebrand Factor; D010450:Endopeptidases