Fidelity of DNA polymerase alpha partially purified from a mutator mutant and wild-type mouse FM3A cells.
Several mutator mutants were isolated from a cultured mouse cell line (FM3A). The mutants exhibited a high rate of spontaneous mutation at three genetic loci of drug resistance. To investigate a possible link between mutator phenotype and fidelity of DNA replication, DNA polymerase alpha was partially purified from the wild-type and the mutator mutant (Fmut 1) showing the highest mutation rate. Using a combination of synthetic template and primer, the ratio of incorporation of incorrect to correct nucleotides was determined. The results indicated that the DNA polymerase alpha from the mutator mutant showed a slightly higher rate of misincorporation, 1.4 and 1.6 times, than that of the wild-type.
Hyodo
M
M
Department of Molecular Biology, School of Medicine, Tokai University, Kanagawa, Japan.
Suzuki
K
K
D000818:Animals; D002460:Cell Line; D004257:DNA Polymerase II / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D003854:Deoxyribonucleotides / Q000378:metabolism; D051379:Mice; D009154:Mutation; D010641:Phenotype
D003854:Deoxyribonucleotides; D004257:DNA Polymerase II