title: Identification, immunoaffinity purification and initial characterization of a novel 71 kD human decidua associated protein by use of specific monoclonal antibodies.
abstract: This study is part of an ongoing attempt to identify and characterize proteins associated with the human decidual tissue. A novel decidual-associated glycoprotein with an apparent molecular weight of 71 kD named hDP71 (human decidual-protein 71), has been identified and purified by immunoaffinity technique using monoclonal antibodies. The monoclonal antibodies recognizing the hDP71 were raised against a partly purified preparation of decidual associated proteins, which was obtained by immunoabsorption of serum proteins from crude decidual extract. Although the hDP71 was copurified with another decidual-associated glycoprotein, the previously described hDP200 (Halperin et al., 1989), evidence is presented showing that the monoclonal antibodies described above are specific for hDP71.
authors.affiliation: Department of Obstetrics and Gynecology, Sackler School of Medicine, Tel Aviv, Israel.
authors.forename: R
authors.initials: R
authors.lastname: Halperin
authors.forename: G
authors.initials: G
authors.lastname: Fleminger
authors.forename: Y
authors.initials: Y
authors.lastname: Ovadia
authors.forename: P F
authors.initials: PF
authors.lastname: Kraicer
authors.forename: E
authors.initials: E
authors.lastname: Hadas
mesh_terms: D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity; D003656:Decidua / Q000737:chemistry*; D004563:Electrochemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007120:Immunochemistry; D008970:Molecular Weight; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D000911:Antibodies, Monoclonal; D011257:Pregnancy Proteins