title: Mass spectrometric determination of isotopically labeled tyrosines and tryptophans in photosynthetic reaction centers of Rhodobacter sphaeroides R-26.
abstract: A synthetic medium for growing Rhodobacter sphaeroides R-26 is developed. This medium opened the way to the preparation of photosynthetic reaction centers incorporated with L-[4'-13C]tyrosine or L-[1'-15N]tryptophan. Gas chromatography combined with mass spectroscopy was used to estimate the metabolic incorporation of the labeled amino acid into the protein. Conditions were found for near-quantitative incorporation of labeled aromatic amino acids into the reaction center.
authors.affiliation: Gorlaeus Laboratories, University of Leiden, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Raap
authors.forename: C
authors.initials: C
authors.lastname: Winkel
authors.forename: A H
authors.initials: AH
authors.lastname: de Wit
authors.forename: A H
authors.initials: AH
authors.lastname: van Houten
authors.forename: A J
authors.initials: AJ
authors.lastname: Hoff
authors.forename: J
authors.initials: J
authors.lastname: Lugtenburg
mesh_terms: D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D007553:Isotope Labeling; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism*; D012242:Rhodobacter sphaeroides / Q000378:metabolism*; D014364:Tryptophan / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D045322:Photosynthetic Reaction Center Complex Proteins; D014443:Tyrosine; D014364:Tryptophan