Cloning of genes for a hemolytic factor of Leptospira interrogans serovar autumnalis strain Congo 21-543.
A DNA fragment encoding a hemolytic factor was cloned from the parasitic spirochete Leptospira interrogans serovar autumnalis strain Congo 21-543. Initial clones were isolated by screening a genomic library in pBR322 in Escherichia coli for hemolytic activity. Hemolytic activity was coded by a 4.5 kilobase BamHI-HindIII fragment. Southern hybridization with DNAs from other strains of Leptospira using this gene as a probe showed that DNAs from non-parasitic strains failed to hybridize with the probe, whereas those from all parasitic strains tested had the sequence which hybridize to the probe.
Fukunaga
M
M
Faculty of Pharmacy and Pharmaceutical Science, University of Fukuyama.
Mifuchi
I
I
Yanagihara
Y
Y
D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis*; D005798:Genes, Bacterial; D006459:Hemolysin Factors / Q000235:genetics*; D007921:Leptospira interrogans / Q000235:genetics* / Q000472:pathogenicity; D015183:Restriction Mapping; D014170:Transformation, Genetic
D004269:DNA, Bacterial