Soluble human lymphocyte sugar binding proteins with immunosuppressive activity.
Suppression of the immune response, which involves suppressor factors released from specialized T cells, is inhibited by alpha-L-rhamnose. In this paper, we show the presence of rhamnose-specific receptors on a human CD8+ T cell-rich population and describe a novel method to isolate cells which express a given sugar-binding protein on their surface. We describe the isolation of alpha-L-rhamnose-specific molecules (rhamnose-binding fractions: RBF) from a water-soluble extract from lymphocytes, their purification by affinity chromatography on immobilized neoglycoproteins containing rhamnose residues. RBF kept their ability to bind rhamnose, as shown by the binding of fluorescein-labeled RBF to rhamnosylated BSA-substituted beads. RBF efficiently suppresses DNA synthesis of mitogen-stimulated human lymphocytes as well as B cell immunoglobulin production. Therefore, these rhamnose-binding molecules appear to be antigen-independent suppressor factors.
Grillon
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Département de Biochimie des Glycoconjugués et Lectines Endogènes, Centre de Biophysique Moléculaire, CNRS, Orléans, France.
Monsigny
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Kieda
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D002352:Carrier Proteins / Q000276:immunology* / Q000302:isolation & purification / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis; D007166:Immunosuppressive Agents / Q000302:isolation & purification* / Q000494:pharmacology; D008863:Microspheres; D011956:Receptors, Cell Surface; D012210:Rhamnose / Q000378:metabolism; D012995:Solubility; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D002352:Carrier Proteins; D007136:Immunoglobulins; D007166:Immunosuppressive Agents; D011956:Receptors, Cell Surface; C055213:saccharide-binding proteins; D004247:DNA; D012210:Rhamnose