Recovery of the contact site A glycoprotein of Dictyostelium discoideum from sodium dodecyl sulfate-polyacrylamide gel and characteristics of monoclonal antibodies against the recovered protein.
Monoclonal antibodies were produced against a cell-cell adhesion (contact site A) glycoprotein of Dictyostelium discoideum, isolated by preparative gel electrophoresis. The glycoprotein was recovered by electroelution from a polyacrylamide gel strip and used for the production of monoclonal antibodies. Four of the five antibodies obtained bound specifically to the protein moiety of the contact site A glycoprotein. The specificities of the antibodies were in striking contrast to those of antibodies raised against the contact site A glycoprotein purified by Triton X-114 phase separation and DEAE chromatography. The majority of the latter antibodies recognized the carbohydrate moiety of the contact site A glycoprotein and cross-reacted heavily with other membrane glycoproteins.
Ochiai
H
H
Department of Botany, Faculty of Science, Hokkaido University, Sapporo.
Jin
K
K
Kakihara
N
N
Saito
T
T
Fukuzawa
M
M
D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000276:immunology / Q000302:isolation & purification*; D003429:Cross Reactions; D004023:Dictyostelium / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000276:immunology / Q000302:isolation & purification*; D008562:Membrane Glycoproteins / Q000276:immunology / Q000302:isolation & purification*; D015800:Protozoan Proteins; D012967:Sodium Dodecyl Sulfate
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D005656:Fungal Proteins; D008562:Membrane Glycoproteins; D015800:Protozoan Proteins; C052291:cell cohesion molecule, Dictyostelium; D012967:Sodium Dodecyl Sulfate