Localization of platelet-derived endothelial cell growth factor in human placenta and purification of an alternatively processed form.
Platelet-derived endothelial cell growth factor (PD-ECGF) was purified to homogeneity from human term placenta, an organ characterized by extensive angiogenesis. N-terminal amino acid sequencing revealed that placental PD-ECGF was proteolytically processed at Thr-6, in contrast to PD-ECGF purified from human platelets, which is processed at Ala-11. The purified factor stimulated porcine aortic endothelial cells as well as two choriocarcinoma cell lines. Immunohistochemical staining revealed that PD-ECGF was present in the connective tissue cells of the placenta. The possibility that PD-ECGF is involved in the development of the placenta is discussed.
Usuki
K
K
Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden.
Norberg
L
L
Larsson
E
E
Miyazono
K
K
Hellman
U
U
Wernstedt
C
C
Rubin
K
K
Heldin
C H
CH
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004727:Endothelium / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D010920:Placenta / Q000737:chemistry* / Q000502:physiology; D010982:Platelet-Derived Growth Factor / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology; D011499:Protein Processing, Post-Translational; D013552:Swine; D014407:Tumor Cells, Cultured
D010982:Platelet-Derived Growth Factor