title: Comparative studies on the degradation of guanidino and ureido compounds by Pseudomonas.
abstract: The utilization of guanidino and ureido compounds was studied in several Pseudomonas species. Multiple routes of agmatine catabolism were found. All members of the homology group I of Pseudomonas use the initial deamination of agmatine to carbamoylputrescine which is subsequently converted to putrescine. In Pseudomonas indigofera, the catabolism of agmatine can also occur via an initial hydrolysis of the amidino group to putrescine catalyzed by an agmatine amidinohydrolase. A third pathway was found in Pseudomonas cepacia, namely oxidative deamination producing guanidinobutyraldehyde catalyzed by agmatine dehydrogenase, followed by formation of guanidinobutyrate and removal of urea by guanidinobutyrate amidinohydrolase to produce 4-aminobutyrate. Novel amidino-hydrolases were characterized in P. putida for the utilization of arcaine and audouine, and in P. cepacia for arcaine, homoarginine and guanidinovalerate. Guanidinovalerate amidinohydrolase was also detected in P. doudoroffii. Some of these amidinohydrolases accept more than one substrate, e.g., guanidinobutyrate and guanidinovalerate utilization by P. doudoroffii and P. cepacia, the catabolism of arcaine and audouine by P. putida, and the degradation of arcaine and homoarginine by P. cepacia.
authors.affiliation: Laboratoire de Microbiologie, Faculté des Sciences, Université Libre de Bruxelles, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Tricot
authors.forename: A
authors.initials: A
authors.lastname: Piérard
authors.forename: V
authors.initials: V
authors.lastname: Stalon
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism; D003401:Creatine / Q000378:metabolism; D003404:Creatinine / Q000378:metabolism; D006146:Guanidines / Q000378:metabolism*; D006868:Hydrolysis; D011549:Pseudomonas / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D014508:Urea / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D006146:Guanidines; D014508:Urea; D003404:Creatinine; D000581:Amidohydrolases; C019946:creatininase; D003401:Creatine