Active release of surface proteins: a mechanism associated with the immune escape of Acanthocheilonema viteae microfilariae.
Living Acanthocheilonema viteae microfilariae obtained from peripheral blood of parasitised Meriones unguiculatus were surface-labelled with 125I. Four major surface exposed proteins of approximately 14.50, 14.55, 17.5, 19 kDa and one less abundant protein of 40 kDa were identified. Under non-reducing conditions the low-molecular-weight (LMW) proteins were isolated as multimers suggesting the presence of intermolecular disulphide linkages. In gels containing Triton X-100 the labelled epicuticular proteins behaved lipophilically. By cultivation of surface-labelled and metabolically labelled microfilaria in vitro, a continuous shedding of two LMW proteins was demonstrated. These proteins were produced in large amounts and released into the culture supernatant as monomeric and pentameric molecules. Concomitant with this release, one of the proteins appeared to lose its lipophilic character, giving rise to a hydrophilic 14.50-kDa entity. Although most of the extracted surface proteins reacted with sera from patent jirds, these sera failed to recognise the surface of living microfilariae. However, microfilariae pretreated with glutaraldehyde or attenuated with Na-azide could be labelled with surface specific antibodies.
Apfel
H
H
Max-Planck-Institut für Biologie, Infektionsbiologie, Tübingen, F.R.G.
Meyer
T F
TF
D000049:Acanthocephala / Q000254:growth & development / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005849:Gerbillinae; D015801:Helminth Proteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D006373:Helminthiasis / Q000276:immunology; D050356:Lipid Metabolism; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D008970:Molecular Weight
D000947:Antigens, Helminth; D015801:Helminth Proteins; D008565:Membrane Proteins