Proliferation of hybridoma cells in low-serum medium.
After stepwise adaptation to PFH-medium with low serum levels over a period of 4 weeks two pools of the IWF-F2 hybridoma have been obtained which can be grown continuously in PFH-medium supplemented with 2 resp. 1% of bovine serum without loss of production of monoclonal antibody. Adaptation of this hybridoma to growth without any serum supplement was not possible during the course of this experimental series.
Sávoly
S B
SB
Institute of Drug Research, Academy of Sciences of the GDR, Berlin.
Kovár
J
J
Rossow
N
N
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D006825:Hybridomas / Q000166:cytology* / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis*; D009101:Multiple Myeloma; D051381:Rats
D003470:Culture Media; D007074:Immunoglobulin G