Determination of tretinoin in creams by high-performance liquid chromatography.
A stability indicating reversed-phase high-performance liquid chromatographic method has been developed to quantify tretinoin (all-trans-retinoic acid) in cream formulations. Tretinoin cream samples were dissolved directly in tetrahydrofuran and diluted for injection. Separation was accomplished on a 15 cm Nova-Pak C18 column using a tetrahydrofuran-phosphate buffer solvent system (42:58, v/v) and 1.0 ml/min flow-rate. The method is able to separate tretinoin from its degradation products formed under stressing conditions. Excellent precision and accuracy were found for the assay of tretinoin in the cream formulations.
Kril
M B
MB
Bristol-Myers Squibb Co., Pharmaceutical Research Institute, Buffalo, NY 14213.
Burke
K A
KA
DiNunzio
J E
JE
Gadde
R R
RR
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003879:Dermatologic Agents / Q000032:analysis*; D009824:Ointments; D014212:Tretinoin / Q000032:analysis*
D003879:Dermatologic Agents; D009824:Ointments; D014212:Tretinoin