[Flow cytome tric analysis of cellular DNA content and cytokinetic study of experimental lung squamous cell carcinoma in rats].
Cellular DNA content in normal lung tissues (group 1, 7 wistar rats), in tissues adjacent to lung cancers (group 2, 8 rats) and in experimental lung squamous cell carcinomas (group 3, 10 rats) was measured by flow cytometry. The results were as follows. Of 10 experimental lung cancers, 8 had aneuploid DNA content, 1 had multiple aneuploid stem lines, and 1 had diploid DNA content. But DNA content in normal lung tissues and in tissues adjacent to experimental lung cancers was all diploidy. Concerning the number of S phase cell and proliferative index, there was significant difference between group 1 and 2 as well as between group 3 and either group 1 or 2.
Zhu
J J
JJ
Department of Pathology, China Medical University, Shenyang.
He
M C
MC
D000782:Aneuploidy; D000818:Animals; D001564:Benzo(a)pyrene; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D002453:Cell Cycle; D002455:Cell Division; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D008175:Lung Neoplasms / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D016196:S Phase
D004273:DNA, Neoplasm; D001564:Benzo(a)pyrene