Low access resistance perforated patch recordings using amphotericin B.
We present experimental procedures describing the creation of perforated patches by use of amphotericin B. In 13 different cellular preparations, access resistances below 10 M omega were achieved and with blunt electrode tips, access resistances of 3-4 M omega were possible. In addition to using the techniques to measure whole cell currents, we have used them to measure single channel currents in a new "outside-out patch" preparation and we have utilized them to measure the resting voltage of epithelial monolayers. We conclude that these new approaches can provide a substantial increase in versatility and quality for many kinds of electrophysiological measurements.
Rae
J
J
Department of Physiology and Biophysics, Mayo Foundation, Rochester, MN 55905.
Cooper
K
K
Gates
P
P
Watsky
M
M
D000666:Amphotericin B / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D001001:Anura; D002478:Cells, Cultured; D004566:Electrodes; D004594:Electrophysiology / Q000295:instrumentation*; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology; D005399:Fishes; D006168:Guinea Pigs; D006801:Humans; D007908:Lens, Crystalline / Q000502:physiology; D051379:Mice; D009761:Nystatin / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D012996:Solutions; D014407:Tumor Cells, Cultured / Q000187:drug effects
D012996:Solutions; D009761:Nystatin; D000666:Amphotericin B