title: Polyol determination along the rat nephron.
abstract: The polyols sorbitol and inositol were determined in single freshly microdissected tubule segments of rat kidney. Twenty different structures were separated from six different kidney zones reaching from cortex to papillary tip. Picomol amounts of sorbitol and inositol were quantitated by use of an enzymatic bioluminescence procedure. Experimental conditions (700 mosmol/kg, 4 degrees C) were chosen to assure constant polyol concentrations over 3 h dissection period. Sorbitol exhibited a concentration gradient in the collecting duct system from the outer/inner medullary border (3.9 +/- 0.5 pmol/mm) to the papillary tip (78.8 +/- 6.9 pmol/mm). In the same region descending and ascending limbs of Henle's loop contained 1.5 +/- 0.5 to 5.3 +/- 1.6 pmol/mm and 2.5 +/- 0.8 to 8.35 +/- 1.5 pmol/mm, respectively. In contrast, all outer medullary and cortical structures had lower sorbitol concentrations. Inositol amounts increased continuously in the collecting duct from cortex (5.3 +/- 0.5 pmol/mm) to inner medulla (30.7 +/- 3.8 pmol/mm). This polyol was also found in thick ascending limb of Henle's loop (6.2 +/- 1.1 pmol/mm in cortex to 11.2 +/- 1.4 pmol/mm in outer medulla) and in proximal tubules (5.6 +/- 1.2 pmol/mm in S1 and 4.5 +/- 1.5 pmol/mm in S3). When related to cellular volume measured by planimetry, intracellular sorbitol concentration was calculated to be 51 mmol/l in papillary collecting duct and inositol 28 mmol/l in outer medullary thick ascending limb cells. These data confirm the role of sorbitol in the renal concentrating process in papilla. Inositol seems to have additional function in thick ascending limb of Henle's loop and the proximal tubule.
authors.affiliation: Institut für Klinische Chemie, Städt. Krankenhaus München Bogenhausen.
authors.forename: M
authors.initials: M
authors.lastname: Schmolke
authors.forename: A
authors.initials: A
authors.lastname: Bornemann
authors.forename: W G
authors.initials: WG
authors.lastname: Guder
mesh_terms: D000818:Animals; D004210:Dissection; D007294:Inositol / Q000032:analysis* / Q000493:pharmacokinetics; D007672:Kidney Cortex / Q000737:chemistry / Q000378:metabolism; D007679:Kidney Medulla / Q000737:chemistry / Q000378:metabolism; D007684:Kidney Tubules / Q000033:anatomy & histology / Q000737:chemistry / Q000378:metabolism; D008163:Luminescent Measurements; D008297:Male; D008832:Microchemistry / Q000379:methods; D009399:Nephrons / Q000737:chemistry* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013012:Sorbitol / Q000032:analysis* / Q000493:pharmacokinetics; D014018:Tissue Distribution
chemical_list: D007294:Inositol; D013012:Sorbitol