Identification of a cell surface component of Swiss 3T3 cells associated with an inhibition of cell division.
Polyclonal IgG, prepared to a purified bovine cell surface sialoglycopeptide (SGP) inhibitor of cell division, was used to identify an antigenically related molecule on the surface of Swiss 3T3 cells. SDS-PAGE and Western analyses showed that the anti-SGP antibody was monospecific and primarily recognized a 66-kDA protein of 3T3 cell membranes. Treatment of intact 3T3 cells or 3T3 cell membranes with either broad and phosphatidylinositol-specific phospholipase C enzymes suggested that the antigenic material most likely existed as an integral membrane molecule, or associated as a multimeric complex, and was not anchored at the cell surface by a phospholipid. The addition of anti-SGP IgG to 3T3 cell monolayer cultures was shown to promote cell division, suggesting a regulatory function for the membrane-associated molecule.
Lakshmanarao
S S
SS
Center for Basic Cancer Research, Kansas State University, Manhattan 66506-4903.
Toole-Simms
W E
WE
Fattaey
H K
HK
Leach
R J
RJ
Johnson
T C
TC
D000818:Animals; D015153:Blotting, Western; D002455:Cell Division / Q000502:physiology*; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D007074:Immunoglobulin G / Q000276:immunology; D008565:Membrane Proteins / Q000032:analysis* / Q000502:physiology; D051379:Mice; D011817:Rabbits; D012795:Sialoglycoproteins / Q000276:immunology
D007074:Immunoglobulin G; D008565:Membrane Proteins; D012795:Sialoglycoproteins