Epithelium-derived linoleic acid metabolites modulate airway smooth muscle function.
Cultured epithelial cells obtained from guinea pig tracheal preparations metabolized arachidonic acid into 5- and 15-hydroxy-eicosatetraenoic acid and into the prostaglandins E2 and F2 alpha. Linoleic acid was converted by the epithelial cells into 9-hydroxy-octadecadienoic acid (9-HODE) and smaller amounts of 13-HODE. It was further investigated whether linoleic acid metabolites are of importance for the regulation of airway smooth muscle function. 13-HODE caused an increase of maximal contraction of tracheal rings to histamine, while 9-HODE had no effect.
Henricks
P A
PA
Department of Pharmacology, Faculty of Pharmacy, University of Utrecht, The Netherlands.
Engles
F
F
van Esch
B
B
van der Linde
H J
HJ
Oosthuizen
M J
MJ
Nijkamp
F P
FP
D000818:Animals; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism / Q000502:physiology; D006632:Histamine / Q000494:pharmacology; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism* / Q000494:pharmacology; D044243:Linoleic Acids, Conjugated; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D014132:Trachea / Q000166:cytology / Q000378:metabolism / Q000502:physiology*
D008041:Linoleic Acids; D044243:Linoleic Acids, Conjugated; C024347:9-hydroxy-10,12-octadecadienoic acid; C024348:13-hydroxy-9,11-octadecadienoic acid; D006632:Histamine; D019787:Linoleic Acid