title: Binding of 2-amino-4-chloro-m-benzenedisulfonamide as a metabolite of hydrochlorothiazide to erythrocytes.
abstract: 2-Amino-4-chloro-m-benzenedisulfonamide (ACBS) is a metabolite of hydrochlorothiazide. We reported that the ACBS concentration in erythrocytes was higher than in plasma in a patient. Therefore the binding of ACBS to rabbit erythrocyte was studied. The Scatchard plot showed the nonlinear plot and the horizontal asymptote. Curvature in this plot indicated the existence of 2 classes of binding. One class was at a specific site, probably at carbonic anhydrase. Chromatographic data seemed to support the possibility.
authors.affiliation: Faculty of Pharmaceutical Sciences, Osaka University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yamazaki
authors.forename: S
authors.initials: S
authors.lastname: Itoh
authors.forename: J
authors.initials: J
authors.lastname: Saji
authors.forename: K
authors.initials: K
authors.lastname: Tanabe
authors.forename: H
authors.initials: H
authors.lastname: Nakahama
authors.forename: Y
authors.initials: Y
authors.lastname: Orita
mesh_terms: D000818:Animals; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D006852:Hydrochlorothiazide / Q000097:blood*; D066298:In Vitro Techniques; D011817:Rabbits; D013424:Sulfanilamides / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D013424:Sulfanilamides; D006852:Hydrochlorothiazide; C054586:4-amino-6-chloro-1,3-benzenedisulfonamide