Inhibition of human immunodeficiency virus forward and reverse transcription by PC6, a natural product from cones of pine trees.
We have previously shown that PC6, a natural product extracted from cones of Pinus parviflora Sieb et Zucc, can inhibit the replication of human immunodeficiency virus type 1 (HIV-1) in CD4+ T cells and in monocyte/macrophage cell lines. Here, we show by immunoprecipitation of HIV-1 proteins with a specific pooled serum that PC6 inhibited the expression of all HIV-1 proteins in CEM cells. PC6 did not affect the posttranslational processing of the HIV-1 proteins. Northern, Southern, and kinetics analyses revealed that PC6 inhibited HIV-1 reverse and forward transcription in CEM cells. Transient transfection of CEM cells with HIV-1 long terminal repeat (LTR) linked CAT DNA also showed that PC6 inhibited LTR-driven gene expression at the transcriptional level.
Takayama
H
H
Laboratory of Virology, Tampa Bay Research Institute, St. Petersburg, FL 33716.
Bradley
G
G
Lai
P K
PK
Tamura
Y
Y
Sakagami
H
H
Tanaka
A
A
Nonoyama
M
M
D000998:Antiviral Agents / Q000494:pharmacology*; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002460:Cell Line; D004279:DNA, Viral / Q000187:drug effects; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000187:drug effects*; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D010936:Plant Extracts / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D011233:Precipitin Tests; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D012367:RNA, Viral / Q000187:drug effects; D014158:Transcription, Genetic / Q000187:drug effects*; D014162:Transfection; D014764:Viral Proteins / Q000096:biosynthesis
D000998:Antiviral Agents; D004279:DNA, Viral; C069800:PC6 extract; D010936:Plant Extracts; D012367:RNA, Viral; D014764:Viral Proteins