title: Determination of amineptine and its main metabolite in plasma by high-performance liquid chromatography after solid-phase extraction.
abstract: Amineptine and its main metabolite were determined simultaneously in plasma by high-performance liquid chromatography using quinupramine as internal standard. The method comprised adsorption on Extrelut column from alkaline plasma, elution with diethyl ether-methylene chloride, evaporation in the presence of 0.01 M hydrochloric acid and injection of the acid solution onto a mu Bondapak C18 column, using acetonitrile-0.025 M potassium dihydrogenphosphate as mobile phase and ultraviolet detection at 210 nm. Average steady-state concentrations of the two compounds were determined in four patients under treatment regimen (two 100-mg doses of amineptine per day, at 8.00 and 12.00 h). The concentrations determined 20 h after the last dose were undetectable in all cases, whereas the concentrations determined 1 h after the second dose were found to be 780 +/- 96 ng ml-1 for amineptine and 690 +/- 137 ng ml-1 for its metabolite. The technique can also be applied to whole blood with, if necessary, identification on the basis of the ultraviolet spectrum obtained by photodiode-array detection.
authors.affiliation: Laboratoire Interrégional de Police Scientifique, Marseille, France.
authors.forename: P P
authors.initials: PP
authors.lastname: Rop
authors.forename: J
authors.initials: J
authors.lastname: Spinazzola
authors.forename: M
authors.initials: M
authors.lastname: Bresson
authors.forename: T
authors.initials: T
authors.lastname: Conquy
authors.forename: A
authors.initials: A
authors.lastname: Viala
mesh_terms: D000327:Adsorption; D000928:Antidepressive Agents / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D003986:Dibenzocycloheptenes / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use; D004986:Ether; D006801:Humans; D008752:Methylene Chloride; D008832:Microchemistry; D008875:Middle Aged; D011786:Quality Control
chemical_list: D000928:Antidepressive Agents; D003986:Dibenzocycloheptenes; D004986:Ether; C011597:amineptin; D008752:Methylene Chloride