Determination of cardenolides in hairy root cultures of Digitalis lanata by enzyme-linked immunosorbent assay.
A competitive ELISA technique using digoxin-specific antibody has been developed to determine digoxin and its related compounds in hairy root cultures of Digitalis lanata. The ELISA could detect 0.2-2 nM digoxin and closely related cardenolides. Hairy roots cultured in the dark accumulated very small amounts of cardenolides (0.02-0.07 micrograms/g dry wt), while the content of cardenolides in green hairy roots cultured in the light was increased maximally 600-fold (16.5 micrograms/g dry wt) compared to those in the dark.
Yoshimatsu
K
K
Tsukuba Medicinal Plant Research Station, National Institute of Hygienic Sciences, Ibaraki, Japan.
Satake
M
M
Shimomura
K
K
Sawada
J I
JI
Terao
T
T
D000911:Antibodies, Monoclonal / Q000276:immunology; D002298:Cardenolides / Q000032:analysis*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D004070:Digitalis / Q000032:analysis*; D004077:Digoxin / Q000032:analysis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D010946:Plants, Medicinal; D010947:Plants, Toxic
D000911:Antibodies, Monoclonal; D002298:Cardenolides; D004077:Digoxin