Effect of tumor necrosis factor on cell proliferation kinetics and sister chromatid exchange frequency in human lymphocytes.
Recombinant human tumor necrosis factor alpha (TNF) was found to cause a significant increase in cell proliferation rates and sister chromatid exchange (SCE) frequency in phytohemagglutinin-stimulated human lymphocytes in vitro. The cells were treated for the entire cultivation time with 10, 50, 100, 500, 1000 and 5000 U/ml TNF. Cell proliferation rates, as measured by the replication index, increased significantly (P less than 0.01) in a concentration-independent manner. The maximal extent of SCE induction (15.18 +/- 0.57 versus 10.26 +/- 0.45 SCEs/cell in control, P less than 0.001) was observed with 50 U/ml TNF.
Lazutka
J R
JR
Ecological Genetics Laboratory, Vilnius University, Lithuania.
Rudaitiene
S
S
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D014409:Tumor Necrosis Factor-alpha; D004247:DNA