title: Characterization of putative growth hormone receptors in human choroid plexus.
abstract: The ability to bind iodine-labelled human growth hormone ([125I]hGH) was measured in different parts of the human brain. The choroid plexus contained the highest amount of binding sites (receptors) and was therefore selected for further studies. The binding between [125I]hGH and the receptor was saturable, of high affinity (Ka = 0.63 nM-1) and pH- as well as time-dependent. After solubilization with Triton X-100 the receptors retained their hormone-binding properties and eluted in the high molecular weight range (greater than 500,000) upon molecular sieve chromatography. Analysis by an affinity cross-linking technique indicated a hormone-binding unit of molecular weight 51,000. The molecular characteristics of the identified binding sites are discussed in comparison to those of growth hormone receptors of human and animal origin.
authors.affiliation: Department of Pharmaceutical Pharmacology, University of Uppsala, Sweden.
authors.forename: Z N
authors.initials: ZN
authors.lastname: Lai
authors.forename: M
authors.initials: M
authors.lastname: Emtner
authors.forename: P
authors.initials: P
authors.lastname: Roos
authors.forename: F
authors.initials: F
authors.lastname: Nyberg
mesh_terms: D000328:Adult; D001921:Brain / Q000378:metabolism; D002831:Choroid Plexus / Q000378:metabolism*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D007700:Kinetics; D008297:Male; D011869:Radioligand Assay; D011986:Receptors, Somatotropin / Q000187:drug effects*; D012737:Sex Factors
chemical_list: D007457:Iodine Radioisotopes; D011986:Receptors, Somatotropin