Drugs as allergens: an immunoassay for detecting IgE antibodies to cephalosporins.
A radioimmunoassay employing cephalothin linked to a solid phase has been developed for the detection of cephalosporin-reactive IgE antibodies. Direct binding and inhibition studies demonstrated allergenic cross-reactivity between cephalosporins and penicillins, and quantitative hapten inhibition experiments identified the 2-thiophene group, and particularly the attached methylene group, of cephalothin as an allergenic determinant.
Harle
D G
DG
Kolling Institute of Medical Research, Royal North Shore Hospital, St Leonards, Australia.
Baldo
B A
BA
D000818:Animals; D002511:Cephalosporins / Q000276:immunology*; D003429:Cross Reactions; D004342:Drug Hypersensitivity / Q000175:diagnosis*; D006241:Haptens / Q000276:immunology; D007073:Immunoglobulin E / Q000032:analysis*; D010406:Penicillins / Q000276:immunology; D011863:Radioimmunoassay; D013329:Structure-Activity Relationship
D002511:Cephalosporins; D006241:Haptens; D010406:Penicillins; D007073:Immunoglobulin E