A human DNA telomeric fragment contains yeast ARS and mitotic stabilizing sequences.
A minilibrary of human DNA fragments was prepared in the vector YIP5 from a DNA preparation enriched for telomeric sequences. Screening of the library produced one clone that hybridized to the TTAGGG sequence. The cloned DNA fragment was shown to be telomeric by a number of criteria. In situ hybridization to metaphase human chromosomes showed that the fragment hybridized to the tips of all human chromosomes. The fragment contained at least two yeast autonomously replicating sequences (ARS) and stabilizing sequences, since it transformed Saccharomyces cerevisiae with high efficiency, giving rise to clones which were mitotically stable under non-selective growth.
Ascenzioni
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Dipartimento di Biologia Cellulare e dello Sviluppo, Università La Sapienza, Roma, Italy.
Della Valle
G
G
Guerrini
A M
AM
Pisani
G
G
Biondi
O
O
Donini
P
P
D001483:Base Sequence; D003001:Cloning, Molecular; D004261:DNA Replication; D004926:Escherichia coli; D015698:Genomic Library; D006801:Humans; D008938:Mitosis; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*