title: ENU mutagenesis in the mouse electrophoretic specific-locus test, 1. Dose-response relationship of electrophoretically-detected mutations arising from mouse spermatogonia treated with ethylnitrosourea.
abstract: The mouse electrophoretic specific-locus test for induced germ-cell mutations, was used to determine the response of spermatogonial stem cells to a series of doses of the germ cell mutagen N-ethyl-N-nitrosourea (ENU). Male DBA/2J and C57B1/6J mice were treated with doses of 50, 100, 200 or 250 mg/kg ENU and their progeny screened for electrophoretically-detectable mutations at 32 separate loci. As expected, increasing doses of ENU led to increasing mutant frequencies. The differences in mutant frequencies between treated DBA/2J and C57B1/6J males were not statistically significant.
authors.affiliation: Research Triangle Institute, Research Triangle Park, NC 27709-2194.
authors.forename: S E
authors.initials: SE
authors.lastname: Lewis
authors.forename: L B
authors.initials: LB
authors.lastname: Barnett
authors.forename: B M
authors.initials: BM
authors.lastname: Sadler
authors.forename: M D
authors.initials: MD
authors.lastname: Shelby
mesh_terms: D000483:Alleles; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004592:Electrophoresis, Starch Gel; D005038:Ethylnitrosourea / Q000633:toxicity*; D005260:Female; D007668:Kidney; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D016296:Mutagenesis; D009152:Mutagenicity Tests; D013093:Spermatogonia / Q000187:drug effects*; D013234:Stem Cells
chemical_list: D005038:Ethylnitrosourea