High-performance liquid chromatographic determination of ciprofloxacin in rat brain and cerebrospinal fluid.
A novel high-performance liquid chromatographic method for the fluorometric determination of a newer quinolone, ciprofloxacin (CPFX), in rat brain and cerebrospinal fluid (CSF) was developed. CPFX in brain homogenate was extracted and injected onto a reversed-phase column without fluorescence derivatization. CSF was directly analyzed without the extraction procedure. Calibration curves were linear over the concentration ranges of 10 to 500 ng/g for brain and 5 to 500 ng/ml for CSF. The recoveries of CPFX added to brain were more than 97% with a coefficient of variation of less than 4%. The present method was sensitive and reliable enough to be utilized for detailed pharmacokinetic studies of CPFX in rat brain and CSF.
Katagiri
Y
Y
Department of Pharmacy, Shimane Medical University Hospital, Japan.
Naora
K
K
Ichikawa
N
N
Hayashibara
M
M
Iwamoto
K
K
D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D002939:Ciprofloxacin / Q000032:analysis* / Q000134:cerebrospinal fluid; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D002939:Ciprofloxacin