Production and characterisation of anti-theophylline monoclonal antibodies suitable for immunoassay.
Spleen cells from BALB/c mice immunised with KLH-theophylline conjugate were fused with a mouse myeloma cell line P3-X63-Ag8.653, and antibody-producing hybrids were identified by enzyme immunoassay. Three cell clones were obtained, each capable of producing a unique monoclonal antibody to theophylline. Using these monoclonal antibodies, an immunoassay system for theophylline was developed.
Danilova
N D
ND
Department of Medical Diagnostics, Institute of Biotechnology, Moscow, U.S.S.R.
Vasilov
R G
RG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D006241:Haptens; D006433:Hemocyanins / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007118:Immunoassay / Q000379:methods*; D051379:Mice; D013806:Theophylline / Q000097:blood / Q000737:chemistry / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006241:Haptens; D006433:Hemocyanins; D013806:Theophylline; C032808:keyhole-limpet hemocyanin