Recovery of impaired gluconeogenesis in kidney-cortex tubules of gentamicin-treated rabbits.
Rabbits were given gentamicin over a period of 10 days. At 1, 3, 5 and 10 days renal proximal tubules were isolated and glucose synthesis from several substrates was measured. A relationship between the inhibition of renal gluconeogenesis, accompanied by a decline of both pyruvate carboxylase and phosphoenopyruvate carboxykinase (PEPCK) activities, and an increased gentamicin level in kidney-cortex was noticed after 5 days of therapy. Both the rates of glucose formation from various substrates as well as pyruvate carboxylase and the cytosolic PEPCK activity recovered fully within 3 weeks after cessation of antibiotic treatment while an increase of activity of the mitochondrial PEPCK occurred during chronic administration of the drug for 10 days. It is concluded, that gentamicin-induced inhibition of gluconeogenesis is one of the events occurring during complex action of this drug on renal cortex.
Michalik
M
M
Institute of Biochemistry, Warsaw University, Poland.
Biedermann
I
I
Lietz
T
T
Bryła
J
J
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D005839:Gentamicins / Q000494:pharmacology*; D005943:Gluconeogenesis / Q000187:drug effects*; D006029:Glycosuria / Q000652:urine; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism*; D007684:Kidney Tubules / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism; D011766:Pyruvate Carboxylase / Q000378:metabolism; D011817:Rabbits
D001786:Blood Glucose; D005839:Gentamicins; D003404:Creatinine; D010729:Phosphoenolpyruvate Carboxykinase (GTP); D011766:Pyruvate Carboxylase