Isolation and characterization of a new abortifacient protein, karasurin, from root tubers of Trichosanthes kirilowii Max. var. japonicum Kitam.
A new abortifacient protein, named karasurin, was isolated from fresh root tubers of Trichosanthes kirilowii MAXIMOWICZ var. japonicum KITAMURA (Cucurbitaceae, Japanese name: kikarasuuri) by the procedure involving acetone fractionation and ion-exchange chromatography on Toyopearlpak SP 650S. Homogeneity of Karasurin was demonstrated by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, and high performance liquid chromatography (HPLC). Karasurin was a highly basic protein of pI 10.1 and the molecular weight was estimated as 28000 by SDS-polyacrylamide gel electrophoresis. Karasurin showed a strong abortion effect in pregnant mice.
Toyokawa
S
S
Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Takeda
T
T
Ogihara
Y
Y
D000020:Abortifacient Agents, Nonsteroidal / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification* / Q000494:pharmacology; D005260:Female; D051379:Mice; D008969:Molecular Sequence Data; D009699:N-Glycosyl Hydrolases; D010940:Plant Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D011247:Pregnancy
D000020:Abortifacient Agents, Nonsteroidal; D004365:Drugs, Chinese Herbal; D010940:Plant Proteins; C069870:karasurin protein, Trichosanthes kirilowii; D009699:N-Glycosyl Hydrolases