[Obtention of Trypanosoma cruzi clones of thin and thick epimastigotes and trypomastigotes living in the blood on various agar media].
Trypanosoma cruzi (EP strain) presents clonal growth when it is incorporated into at 0.7% agar and at 0.8% in surface. The colonies were obtained from a single parasite and in a relatively short time. The efficiency of plating was variable and depended on the type of agar medium. A maximal efficiency was obtained for at 0.7 agar/LIT-BHI and for at 0.8% agar/LIT-BHI-Blood. The sanguicolous tripomastigotes, either thin or thick, were able to generate colonies from single parasites, with 100% plating efficiency, higher than 80% corresponding to the epimastigotes coming from LIT medium.
Concepción
J L
JL
Facultad de Ciencias, Universidad de los Andes, Mérida, Venezuela.
López
N
N
Dagert
M
M
D000362:Agar; D000818:Animals; D010274:Parasitology / Q000379:methods; D014349:Trypanosoma cruzi / Q000254:growth & development / Q000302:isolation & purification*
D000362:Agar
Obtención de clones de Trypanosoma cruzi a partir de epimastigotes y tripomastigotes sanguícolas delgados y gruesos en diferentes medios agarizados.