[Characterization of erythrocyte deformability by filtration technics. 5. The effect of leukocytes on the filterability of erythrocytes].
Filterability measurements have been used widely in medical research and clinical practice to characterize erythrocyte deformability. In the present paper the influence of white blood cells (WBC) on the of filterability indices is investigated and discussed. We found for two gravity filtration systems using cellulose filters and concentrated erythrocyte suspensions sensitivities of their filterability indices of 1% per 100 WBC/microliters (initial) filtration rate measurement) and 3% per 100 WBC/microliters (hemofiltrometer). The necessity, advantages and disadvantages of additional methods for WBC removal are discussed in comparison to nuclepore filtration systems with sensitivities of 5-12% per 100 WBC/microliters. The standardization of residual WBC-count in limits of 500 +/- 300 WBC/microliters at a hematocrit of 60% is proposed.
Kucera
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Abteilung Hämorheologie und Hämodynamik, Medizinischen Fakultät (Charité), Humboldt-Universität zu Berlin.
Wegner
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G
Lerche
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D
D004907:Erythrocyte Deformability; D004912:Erythrocytes / Q000166:cytology / Q000502:physiology; D005374:Filtration / Q000379:methods; D006400:Hematocrit; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes / Q000166:cytology / Q000502:physiology*
Charakterisierung der Erythrozytendeformierbarkeit mittels Filtrationstechnik. 5. Mitteilung: Der Einfluss von Leukozyten auf die Filtrierbarkeit von Erythrozyten.