Inactivation of strongyloides stercoralis filariform larvae in vitro by six Jamaican plant extracts and three commercial anthelmintics.
In vitro bioassay of (a) aqueous methanol extracts (AME) of the green leaves of mimosa (Mimosa pudica), love weed (Cuscuta americana), vervine (Stachytarpheta jamaicensis), chicken weed (Salvia serotina) and breadfruit (Artocarpus altilis); (b) methanol-water fraction (MWF) of breadfruit leaves, and (c) commercially available drugs albendazole, thiabendazole and levamisole were assayed for nematode inactivating potential, using filariform larvae of Strongyloides stercoralis. Test larvae were obtained from a 10-day-old charcoal coproculture. Bioassays were conducted in Locke's solution, using 100 larvae in each of three replicates. Inactivation was recorded microscopically at 1, 3, 6 and 12 hours, then every 24 hours up to 5 days' incubation. It50 (time for inactivation of 50% of larvae) values read: levamisole and mimosa extract less than 1 hour; love weed extract, approximately 2 hours; breadfruit (MWF), 9.5 hours; chicken weed, 20 hours; albendazole, 35 hours; breadfruit (AME), 49 hours; thiabendazole, 74 hours and vervine extract, 81.5 hours. It95 values followed a similar, trend, and were approximately double the It50 measures. A potential role for locally available natural products in the treatment of strongyloidiasis is highlighted.
Robinson
R D
RD
Department of Zoology, U.W.I., Jamaica.
Williams
L A
LA
Lindo
J F
JF
Terry
S I
SI
Mansingh
A
A
D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D001681:Biological Assay; D005243:Feces / Q000469:parasitology; D006801:Humans; D007563:Jamaica; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D013321:Strongyloides / Q000187:drug effects* / Q000254:growth & development
D000871:Anthelmintics; D010936:Plant Extracts