Immunodiagnosis of taeniasis by coproantigen detection.
Immunodiagnostic tests for Taenia-specific faecal antigen based on polyclonal rabbit antisera against Taenia saginata or Taenia solium proglottid extracts in capture-type ELISA assays have been developed. Taenia-specific antigen was detected in detergent-solubilized faecal extracts from T. solium- and T. saginata-infected hosts. Coproantigen from T. solium-infected hamsters did not cross-react with faeces from rodents infected with Hymenolepis diminuta, H. citelli, H. microstoma, Necator americanus, Strongyloides ratti or Nematospiroides dubius and faeces from uninfected animals. When the T. saginata-capture ELISA was tested with faecal samples positive for T. solium antigen, no cross-reactions were obtained. However, faecal samples from humans infected with T. solium or T. saginata, including some with extremely low egg counts, were cross-reactive by either test. Nevertheless, considerably higher O.D. values were obtained with stool samples from Taenia patients compared to Hymenolepis nana-infected or uninfected individuals. Two individuals, infected with Taenia sp. and positive for coproantigens by ELISA, became antigen-negative 6 days after treatment with Niclosamide. The possibility of developing species-specific immunodiagnostic tests for human taeniasis through coproantigen detection is discussed.
Allan
J C
JC
Department of Parasitology, Liverpool School of Tropical Medicine, UK.
Avila
G
G
Garcia Noval
J
J
Flisser
A
A
Craig
P S
PS
D000293:Adolescent; D000328:Adult; D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D006224:Cricetinae; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology*; D005260:Female; D006801:Humans; D008297:Male; D008647:Mesocricetus; D011237:Predictive Value of Tests; D013621:Taenia / Q000276:immunology*; D013622:Taeniasis / Q000175:diagnosis*
D000947:Antigens, Helminth