title: Genetic analysis of functional differences among distinct ferredoxins in Rhodobacter capsulatus.
abstract: Rhodobacter capsulatus has been known to possess two ferredoxins (I and II) with distinct physicochemical and structural properties: ferredoxin I is a 2[4Fe-4S] type and the other is a [3Fe-4S] [4Fe-4S] type. To analyze their possible functional differences, their genes (fdxN and fdxA) were cloned, sequenced, and subjected to interposon mutagenesis experiments. The former gene was adjacent to a gene encoding a chloroplast-type [2Fe-2S] ferredoxin (fdxC). Mutants with inactivated fdxN and/or fdxC were obtained, and they showed virtually no growth under nitrogen-fixing conditions. Complementation experiments confirmed that both fdxN and fdxC were required for nitrogen fixation. On the other hand, we have not been able to disrupt fdxA under the screening conditions surveyed, including conditions that do not require nitrogenase activity for growth, suggesting that ferredoxin II could have an unknown essential role(s). These indicate functional differences among multiple ferredoxins in one bacterium other than in cyanobacterial heterocysts and indispensability of certain ferredoxins in nitrogen fixation other than Rhizobium meliloti FdxN.
authors.affiliation: Department of Biology, Faculty of Science, Osaka University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Saeki
authors.forename: Y
authors.initials: Y
authors.lastname: Suetsugu
authors.forename: K
authors.initials: K
authors.lastname: Tokuda
authors.forename: Y
authors.initials: Y
authors.lastname: Miyatake
authors.forename: D A
authors.initials: DA
authors.lastname: Young
authors.forename: B L
authors.initials: BL
authors.lastname: Marrs
authors.forename: H
authors.initials: H
authors.lastname: Matsubara
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003360:Cosmids; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005288:Ferredoxins / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames; D015183:Restriction Mapping; D016354:Rhodobacter capsulatus / Q000235:genetics* / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D005288:Ferredoxins