Preparative free-flow isoelectric focusing: modeling and experiments.
Free-flow isoelectric focusing was adapted to preparative scale separations and chemical engineering methods were used to describe the main mechanisms operating in the apparatus. A mixture of human serum albumin (pI 4.6) and beta-lactoglobulin (pI 5.22) was separated in pH gradients, generated with carrier ampholytes of different origin and covering the pH ranges 4-6.5, 3.5-5, 4-5.5 and 4.5-5.0. Best results were obtained in the pH 4-5.5 range. The experimental results have validated the results obtained with a numerical model.
Poux
M
M
Laboratoire de Génie Chimique, URA CNRS, Toulouse, France.
Bertrand
J
J
D000665:Ampholyte Mixtures; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D007782:Lactoglobulins / Q000737:chemistry; D015233:Models, Statistical; D015203:Reproducibility of Results; D012709:Serum Albumin / Q000737:chemistry
D000665:Ampholyte Mixtures; D007782:Lactoglobulins; D012709:Serum Albumin