Reaction of the rabbit corneal endothelium to nylon sutures. A SEM study.
Nylon and stainless steel sutures separately placed deeply into rabbit corneas by splitting the stroma for a few millimeters, without closing sutures, remained in the cornea for two, four and six weeks respectively. In contrast to the stainless steel sutures an extensive tissue reaction could be observed clinically around the nylon sutures within a few days and was still present after 4-6 weeks. On the endothelial side, covering of the nylon with fibroblast cells took place very slowly, this in contrast with stainless steel. Irritation of the tissue and an oedematous appearance of the endothelium around the nylon suture was the result. At first a kind of collagen network, often mixed with inflammatory cells, was deposited on the nylon material before fibroblast cells could grow in. Covering of the stainless steel started quite soon after implantation, without preliminary deposition of collagen material. The reason for this phenomenon must be sought in the high free surface energy of the stainless steel, which attracts cells, in contrast to nylon which has a very low free surface energy.
Jongebloed
W L
WL
Department of Histology and Cellbiology, University of Groningen, The Netherlands.
van der Veen
G
G
Kalicharan
D
D
Rijneveld
W J
WJ
Houtman
W A
WA
Worst
J G
JG
D000818:Animals; D002465:Cell Movement; D003315:Cornea / Q000601:surgery / Q000648:ultrastructure*; D005347:Fibroblasts; D008855:Microscopy, Electron, Scanning; D009757:Nylons / Q000009:adverse effects*; D011817:Rabbits; D013193:Stainless Steel; D013537:Sutures; D014945:Wound Healing
D009757:Nylons; D013193:Stainless Steel