title: Effects of prepubertal castration on the spinal motor nucleus of the ischiocavernosus muscle of the rat.
abstract: The location, number and size of the motoneurons innervating the ischiocavernosus muscle, identified by means of horseradish-peroxidase (HRP) retrograde transport, were studied (1) in adult untreated male rats, (2) in adult male rats castrated before puberty, and (3) in adult male rats castrated before puberty and injected with testosterone from the day of castration. After injection of HRP into the ischiocavernosus muscle, labeled motoneurons were found in the dorsolateral and dorsomedial columns of the lamina IX, at the level of L6 and S1 segments of the spinal cord. Morphometric analysis demonstrated that prepubertal castration induces a statistically significant reduction in the somatic and nuclear areas (40% and 35%, respectively, if compared to those of the control rats) of both the dorsolateral and dorsomedial motoneurons, but does not affect their number. The effects of castration are prevented by exogenous testosterone.
authors.affiliation: Department of Human Anatomy and Physiology, Section of Neuroanatomy and Neuroembryology, Torino, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Vercelli
authors.forename: C
authors.initials: C
authors.lastname: Cracco
mesh_terms: D000818:Animals; D007124:Immunoenzyme Techniques; D007267:Injections; D008297:Male; D051379:Mice; D009046:Motor Neurons / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*; D009132:Muscles / Q000294:innervation*; D009919:Orchiectomy; D010502:Perineum; D051381:Rats; D011919:Rats, Inbred Strains; D012741:Sexual Maturation / Q000502:physiology*; D013127:Spinal Nerves / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*; D013739:Testosterone / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D013739:Testosterone