Preparative scale isolation of sphingosine.
A high-yield method is described for the preparation of sphingenine from galactosylceramide. Two successive cleavage reactions are used, alkaline butanol to form galactosylsphingosine, then acidic acetonitrile to form the sphingenine. A column purification procedure utilizing silica gel is described. The method is superior to previously described methods in its rapidity and avoidance of isomerization and contamination by racemization and rearrangement products.
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Mental Health Research Institute, University of Michigan, Ann Arbor 48109.
D000097:Acetonitriles; D000440:Butanols; D002855:Chromatography, Thin Layer / Q000379:methods*; D005699:Galactosylceramides / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D013110:Sphingosine / Q000302:isolation & purification*
D000097:Acetonitriles; D000440:Butanols; D005699:Galactosylceramides; D013110:Sphingosine