title: [Purification and properties of the hexon antigen of canine adenovirus serotype CAV-1].
abstract: The main antigen and immunogen of canine adenovirus type 1 (CAV-1) has been purified to near homogeneity from cultural fluid of a CAV-1-infected primary cell culture by hydrophobic and anion-exchange chromatography. The hexon native form (trimer) was shown to be resistant against denaturation by SDS under conditions of SDS-PAGE performed without heating the samples. The monomer chain of the CAV-1 hexon was apparently identical in terms of electrophoretic mobility with that of the previously sequenced BAV-3 hexon polypeptide (103 kDA). In blot enzyme immunoassay only native trimers of CAV-1 hexon were detected by cross-specific polyclonal and monoclonal anti-hexon antibodies.
authors.forename: L V
authors.initials: LV
authors.lastname: Verkhovskaia
authors.forename: V N
authors.initials: VN
authors.lastname: Ulasov
authors.forename: E K
authors.initials: EK
authors.lastname: Kiseleva
authors.forename: S N
authors.initials: SN
authors.lastname: Khil'ko
authors.forename: T I
authors.initials: TI
authors.lastname: Tikhonenko
mesh_terms: D000256:Adenoviridae / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D015153:Blotting, Western; D002213:Capsid / Q000737:chemistry / Q000302:isolation & purification*; D036022:Capsid Proteins; D002852:Chromatography, Ion Exchange; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus
vernacular_title: Ochistka i svoĭstva geksonobogo antigena adenovirusa sobak serotipa CAV-1.