title: Clonorchis sinensis: purification and characterization of a cysteine proteinase from adult worms.
abstract: 1. Adult Clonorchis sinensis, the Chinese liver fluke, is known to migrate to the bile ducts of its mammalian host and cause significant pathology. 2. An acidic, thiol-dependent proteinase with a native mol. wt of approximately 18,500 was purified to homogeneity using ion-exchange chromatography and gel filtration chromatography. By SDS-polyacrylamide gel electrophoresis, the mol. wt of the enzyme was estimated to be 15,000. 3. The enzyme was similar to cathepsin B-like cysteine proteinases based on pH optimum, substrate specificity, and inhibitor sensitivity. 4. Antisera from human clonorchiasis and C. sinensis-infected rabbits reacted in immunoblots with the partially purified proteinase. The C. sinensis proteinase may be useful for serodiagnosis of clonorchiasis.
authors.affiliation: Department of Biology, Chung-Ang University, Seoul, Korea.
authors.forename: C Y
authors.initials: CY
authors.lastname: Song
authors.forename: M H
authors.initials: MH
authors.lastname: Dresden
authors.forename: A A
authors.initials: AA
authors.lastname: Rege
mesh_terms: D000818:Animals; D002401:Cathepsin B / Q000378:metabolism; D002845:Chromatography; D003004:Clonorchis sinensis / Q000201:enzymology*; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D004229:Dithiothreitol / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D015151:Immunoblotting; D008970:Molecular Weight; D011480:Protease Inhibitors / Q000494:pharmacology; D013379:Substrate Specificity
chemical_list: D011480:Protease Inhibitors; D003546:Cysteine Endopeptidases; D002401:Cathepsin B; D004229:Dithiothreitol