Expression of HTLV-I envelope protein fused to hydrophobic amino-terminal peptide of baculovirus polyhedrin in insect cells and its application for serological assays.
The envelope of human T-cell leukemia virus type I (HTLV-I) consists of two glycoproteins gp46 and p20E. Recombinant envelope proteins were produced by using an expression vector derived from insect baculovirus, Bombyx mori nuclear polyhedrosis virus. Polyhedrin fusion proteins C182, N147, and N287 contained whole region p20E, C-terminal half of gp46, and almost whole region gp46, respectively. N147 and N287 were suggested to be processed forms resulting from internal cleavage by cellular enzymes. In cultured cells and the insect larvae, C182 and N147 were produced abundantly enough to be purified to homogeneity; however, N287 was produced poorly and not purified. The purified proteins were recognized by HTLV-I-infected human sera and shown to be highly specific antigens for blood screening systems.
Nyunoya
H
H
Virology Division National Cancer Center Research Institute, Tsukiji, Japan.
Ogura
T
T
Kikuchi
M
M
Iwamoto
H
H
Yamashita
K
K
Maekawa
M
M
Takebe
Y
Y
Miyamura
K
K
Yamazaki
S
S
Shimotohno
K
K
D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D001483:Base Sequence; D012831:Bombyx; D002460:Cell Line; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D015686:Gene Products, env; D005822:Genetic Vectors; D006168:Guinea Pigs; D015481:HTLV-I Antibodies / Q000097:blood / Q000276:immunology; D015490:HTLV-I Infections / Q000276:immunology; D015368:Human T-lymphotropic virus 1 / Q000235:genetics* / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D000077183:Occlusion Body Matrix Proteins; D011993:Recombinant Fusion Proteins / Q000276:immunology; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics* / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology; D015678:Viral Structural Proteins
D015686:Gene Products, env; D015481:HTLV-I Antibodies; D000077183:Occlusion Body Matrix Proteins; C067985:P20E protein, Human T-lymphotropic virus 1; D011993:Recombinant Fusion Proteins; D015682:Retroviridae Proteins, Oncogenic; D014759:Viral Envelope Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C055266:gp46 protein, Human T-cell leukemia virus type I; C009829:polyhedrin protein, Nucleopolyhedrovirus