GDA-J/F3 monoclonal antibody as a novel probe for the human sperm tail fibrous sheath and its anomalies.
GDA-J/F3 monoclonal antibody (MoAb) recognized an antigen in the fibrous sheath (FS) of human spermatozoa. This was based on: (i) intracellular localization of the antigen which was limited to the principal piece of the sperm tail; (ii) its absence from the cilia of trachea and nasal mucosa which lack FS; (iii) immunogold electron microscopy (IEM) which confirmed its ultrastructural localization to the FS. The antibody was used for the detection of abnormal germ cells in human semen. Nucleated cells other than spermatozoa (NCOS) obtained from oligozoospermic donors were screened with GDA-J/F3 MoAb using the indirect immunofluorescence test. The antibody which stains only the tails in normal cells, produced diffuse cytoplasmic immunofluorescence inside some spermatids. Using phase contrast microscopy, the tails in these spermatids were either present or undetectable. Electron microscopy studies of the NCOS showed the lack of the FS in those which had the tails (afibrous tail), or the presence of disorganized tails (tail dysgenesis) in the others. This antibody therefore provides a useful analytical tool for probing the FS as well as for the easy identification of certain abnormal germ cells in human semen.
Jassim
A
A
Department of Immunology, London Hospital Medical College, UK.
Auger
D
D
Oliver
T
T
Sachs
J
J
D000911:Antibodies, Monoclonal; D002923:Cilia / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D016253:Microscopy, Immunoelectron; D009666:Nose / Q000166:cytology; D013082:Sperm Tail / Q000276:immunology / Q000648:ultrastructure*; D014132:Trachea / Q000166:cytology
D000911:Antibodies, Monoclonal