Calcium mobilization in activated mast cells monitored by flow cytometric analysis.
Changes in intracellular calcium levels induced in mast cells by either antigen or ionomycin were monitored using flow cytometry and the calcium binding dye indo-1. Both stimuli increased calcium levels in responsive cells by a similar amount, but not all cells examined were responsive to antigen. Antigen-induced increases in intracellular calcium levels could be completely blocked by the calcium antagonist TMB-8. Flow cytometry appears to be a useful method for monitoring mast cell calcium concentrations, as it provides the capability to study large numbers of individual cells.
Rossi
G L
GL
Department of Medicine, University of California, San Diego School of Medicine.
Young
D J
DJ
Wasserman
S I
SI
Barrett
K E
KE
D000818:Animals; D000941:Antigens / Q000276:immunology; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002478:Cells, Cultured; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D007211:Indoles; D015759:Ionomycin / Q000494:pharmacology; D007700:Kinetics; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D051379:Mice
D000941:Antigens; D002121:Calcium Channel Blockers; D005456:Fluorescent Dyes; D007211:Indoles; D015759:Ionomycin; C006014:8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate; D005707:Gallic Acid; C048960:indo-1; D002118:Calcium