Uptake of antitetanus F(ab')2 fragments into eukaryotic cells.
1. In order to introduce antitetanus immunoglobulin fragments into eukaryotic cells, either antitetanus F(ab')2 or Fab' fragments have been linked to carrier molecules. Aciclovir, horseradish peroxidase, wheat germ agglutinin, and transferrin were tried as carriers. 2. F(ab')2-aciclovir and Fab'-horseradish peroxidase were not internalized by NG108-15 neurohybridoma cells. 3. [Fab']2-wheat germ agglutinin and F(ab')2-transferrin conjugates were internalized into various cells. 4. F(ab')2-transferrin conjugates were made with three different linkers: N-succinimidyl 3-(2-pyridyldithio) propionate, bis-maleimido hexane, and bis-maleimidoethoxy propane. All three conjugates were internalized but had a different fate inside the cells.
Wellhöner
H H
HH
Department of Toxicology, Medical School of Hannover, FRG.
Bigalke
H
H
Borcholte
T
T
Erdmann
G
G
Eschenhagen
T
T
Jung
K H
KH
Marxen
P
P
Peukert
U
U
Neville
D M
DM
Srinivasachar
K
K
D000212:Acyclovir / Q000276:immunology; D005057:Eukaryotic Cells / Q000276:immunology*; D006735:Horseradish Peroxidase / Q000276:immunology; D007140:Immunoglobulin Fab Fragments; D000922:Immunotoxins / Q000276:immunology; D013743:Tetanus Antitoxin / Q000276:immunology*; D014168:Transferrin / Q000276:immunology; D014909:Wheat Germ Agglutinins / Q000276:immunology
D007140:Immunoglobulin Fab Fragments; D000922:Immunotoxins; D013743:Tetanus Antitoxin; D014168:Transferrin; D014909:Wheat Germ Agglutinins; D006735:Horseradish Peroxidase; D000212:Acyclovir