Oscillatory reaction of alcohol dehydrogenase in an oil/water system.
With the use of an oil/water system, oscillatory reactions of an enzyme have been demonstrated. This reaction system has been conceived as an example of the metabolic oscillations of living cells. When a substrate (ethanol) in the oil phase of toluene or chloroform slowly migrated into the aqueous phase containing alcohol dehydrogenase and NAD+, oscillations were observed in the concentration of NADH produced. The gradual entry of substrate into the aqueous phase was essential for the oscillatory reactions to occur. A possible mechanism to account for the appearance of oscillatory reactions of enzymes is proposed, which differs from that presented previously.
Hideshima
T
T
College of Arts and Sciences, Chiba University, Japan.
D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D001683:Biological Clocks; D002725:Chloroform / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D006019:Glycolysis; D007700:Kinetics; D008954:Models, Biological; D009243:NAD / Q000378:metabolism; D009821:Oils / Q000378:metabolism*; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D014050:Toluene / Q000378:metabolism; D014867:Water / Q000378:metabolism*
D009821:Oils; D014867:Water; D009243:NAD; D014050:Toluene; D000431:Ethanol; D002725:Chloroform; D000426:Alcohol Dehydrogenase