title: The unusual action of (R,S)-2-hydroxy-2-trifluoromethyl-trans-n-octadec-4-enoic acid on 5-lipoxygenase from potato tubers.
abstract: We found that (R,S)-2-hydroxy-2-trifluoromethyl-trans-n-octadec-4-enoic acid (HTFOA) is a powerful activator of 5-lipoxygenase from potato tubers. The degree of activation of the enzyme is proportional to the HTFOA concentration and is a maximum at about 0.1 mM independently of initial substrate concentration (25 microM or 0.1 mM). At greater concentrations of HTFOA, enzyme inhibition takes place. Enzyme activation is inversely proportional to the substrate (linoleic acid) concentration. The results may be explained by assuming that a regulatory center exists in the enzyme molecule, which shows affinity to both substances: activator and linoleic acid.
authors.affiliation: Institute of Bioorganic Chemistry, Ukrainian Academy of Sciences, Kiev, USSR.
authors.forename: I A
authors.initials: IA
authors.lastname: Butovich
authors.forename: V A
authors.initials: VA
authors.lastname: Soloshonok
authors.forename: V P
authors.initials: VP
authors.lastname: Kukhar
mesh_terms: D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism*; D004789:Enzyme Activation; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry / Q000494:pharmacology*; D006880:Hydroxy Acids / Q000737:chemistry / Q000494:pharmacology*; D007700:Kinetics; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000737:chemistry; D010084:Oxidation-Reduction; D011198:Solanum tuberosum / Q000201:enzymology*
chemical_list: D005229:Fatty Acids, Monounsaturated; D006880:Hydroxy Acids; D008041:Linoleic Acids; C069772:2-hydroxy-2-trifluoromethyloctadec-4-enoic acid; D019787:Linoleic Acid; D001094:Arachidonate 5-Lipoxygenase