C7*9, a new frequent C7 allele detected by an allotype-specific monoclonal antibody.
Two sandwich ELISA for the quantitation of C7 in human plasma--one based on a monoclonal (MAb), the other performed with a polyclonal anti-C7 IgG--were established. Results from ELISA as well as from isoelectric focussing and immunoblotting with both antibodies revealed that the MAb is directed to an allotypic epitope on C7. About 6.7% of the plasma samples did not react with this MAb. Since these findings cannot be explained by the known alleles, the existence of a new C7 allele, C7*9, was suggested, whose product C7-9 is not detected by the MAb. A gene frequency of 0.21 C7*9 was assumed. When using conventional techniques C7*9 is included in the frequency of C7*1.
Würzner
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Department of Immunology, Georg August University, Göttingen, FRG.
Nitze
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Götze
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D000483:Alleles; D000906:Antibodies; D000911:Antibodies, Monoclonal; D003184:Complement C7 / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005787:Gene Frequency; D006801:Humans; D015151:Immunoblotting; D007525:Isoelectric Focusing; D010641:Phenotype; D011110:Polymorphism, Genetic
D000906:Antibodies; D000911:Antibodies, Monoclonal; D003184:Complement C7