[Enzyme immunoassay for the determination of albumin in urine].
Two modifications of a double antibody enzyme immunoassay for the determination of urinary albumin content are described. The method is simple, rapid and precise and can be carried out in test tubes and on microtiter plates as well. In 1:10 diluted urine samples albumin concentrations of 1.25 to 20 mg/l (corresponding to the normal range) can be determined. For a control sample with 0.3 mg/l albumin the intra- and interassay coefficients of variation were 4.9% (n = 11) and 10.4% (n = 21), respectively, on microtiter plates.
Gottschling
H D
HD
Institut für Diabetes Gerhardt Katsch Karlsburg.
Jahr
D
D
Diaz-Alonso
J H
JH
Keilacker
H
H
Besch
W
W
Werner
S
S
Ziegler
M
M
D000419:Albuminuria; D000906:Antibodies; D003922:Diabetes Mellitus, Type 1 / Q000652:urine; D006801:Humans; D007124:Immunoenzyme Techniques; D008832:Microchemistry
D000906:Antibodies
Enzymimmunoassay zur Bestimmung von Albumin im Urin.