Expression of the Aspergillus niger glucose oxidase gene in A. niger, A. nidulans and Saccharomyces cerevisiae.
We report the cloning of the Aspergillus niger glucose oxidase gene and its use to elevate glucose oxidase productivity in A. niger by increasing the gene dosage. In addition, the gene has been introduced into A. nidulans where it provides the novel capacity to produce glucose oxidase. A plasmid, in which DNA encoding the mature form of glucose oxidase was preceded by a Saccharomyces cerevisiae secretion signal, effected high-level production of extracellular glucose oxidase in this yeast.
Whittington
H
H
Department of Botany, University of Nottingham, UK.
Kerry-Williams
S
S
Bidgood
K
K
Dodsworth
N
N
Peberdy
J
J
Dobson
M
M
Hinchliffe
E
E
Ballance
D J
DJ
D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000235:genetics*; D001234:Aspergillus niger / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D004271:DNA, Fungal; D015870:Gene Expression; D005800:Genes, Fungal; D005949:Glucose Oxidase / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004271:DNA, Fungal; D005949:Glucose Oxidase