[The dependence of the formation of stromal CFU-f colonies on the stimulating action of hematopoietic cells].
CFU-f-derived stromal colony formation was accomplished in adherent marrow cell cultures (AMCC) with serum-rich medium. It turned out to require additional stimulation by hemopoietic feeder cells: by irradiated marrow cells and spleen cells if they possess megakaryocytes and platelets or by platelets from the blood. PDGF, EGF and IL-3 did not substitute the colony stimulating activity of feeder cells. Thymus, lymph node cells and blood leucocytes had no colony stimulating activity. At low oxygen concentrations which improve colony formation the stimulating activity of hemopoietic feeder cells was expressed, as well. Thus, CFU-f colony formation depends on stimulation by hemopoietic cells in addition to serum growth factors. In full populations of marrow cells the CFU-f colony formation is stimulated by marrow cells which accompany the CFU-f.
Latsinik
N V
NV
Fridenshteĭn
A Ia
AIa
Gorskaia
Iu F
IuF
Moskvina
I L
IL
Iuĭ
Kh Ch
KhCh
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000528:radiation effects; D001854:Bone Marrow Cells; D002448:Cell Adhesion / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D003114:Colony-Forming Units Assay / Q000379:methods; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D006168:Guinea Pigs; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D007377:Interleukin-3 / Q000494:pharmacology; D051379:Mice; D008808:Mice, Inbred CBA; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology
D007377:Interleukin-3; D010982:Platelet-Derived Growth Factor; D004815:Epidermal Growth Factor
Zavisimost' obrazovaniia stromal'nykh KOKf-koloniĭ ot stimuliruiushchego vozdeĭstviia gemopoéticheskikh kletok.