Reactivity of Limulus amoebocyte lysate towards (1----3)-beta-D-glucans.
The structure activity relationship for beta-D-glucans for the gelation of the amoebocyte lysates of the horseshoe crab (Limulus) has been investigated. beta-D-Glucans that had no (1----3) linkages induced little or no gelation. The (1----3)-beta-D-glucans curdlan (unbranched), grifolan (approximately 33% branched), schizophyllan (approximately 33% branched), lentinan (approximately 40% branched). SSG (approximately 50% branched), and OL-2 (approximately 66% branched) induced significant gelation. The optimum concentration for gelation was correlated with the content of branching. Single chain (rather than a triple helix) conformation and higher molecular weight were associated with higher reactivity.
Ohno
N
N
Laboratory of Immunopharmacology of Microbial Products, Tokyo College of Pharmacy, Japan.
Emori
Y
Y
Yadomae
T
T
Saito
K
K
Masuda
A
A
Oikawa
S
S
D000818:Animals; D002236:Carbohydrate Conformation; D005936:Glucans / Q000737:chemistry / Q000378:metabolism*; D008033:Limulus Test; D008970:Molecular Weight; D013329:Structure-Activity Relationship; D047071:beta-Glucans
D005936:Glucans; D047071:beta-Glucans; C033363:beta-1,3-glucan