title: [Effect of various factors during cryopreservation on the morphology and viability of several poikilothermic cell lines].
abstract: This paper deals with the effect of several factors, during cryopreservation, on three poikilothermic cell lines using morphology and viability as indicators. It was proved that to avoid toxicity in the suspension medium is of vital importance for the outcome of freezing. In addition, one of the systems studied and of great application in Virology does not bear long hibernations. Finally, the most adequate freezing method for this type of cells is discussed.
authors.affiliation: Instituto de Medicina Tropical Pedro Kouri, Ciudad de La Habana, Cuba.
authors.forename: A
authors.initials: A
authors.lastname: Castillo Alvarez
authors.forename: L
authors.initials: L
authors.lastname: Morier Días
authors.forename: V
authors.initials: V
authors.lastname: Pérez Forcada
authors.forename: I
authors.initials: I
authors.lastname: Lezcano Jiménez
mesh_terms: D002460:Cell Line / Q000166:cytology* / Q000502:physiology*; D002470:Cell Survival; D003080:Cold Temperature; D015925:Cryopreservation / Q000379:methods*; D013997:Time Factors
vernacular_title: Efecto de varios factores durante la criopreservación sobre la morfología y la viabilidad de algunas líneas celulares poikilotérmicas.