Molecular karyotype of Entamoeba histolytica and Entamoeba invadens.
We report the size-fractionation of Entamoeba histolytica and E. invadens deoxyribonucleic acid (DNA) by pulsed field gradient electrophoresis. Using 3 different electrophoretic conditions, we were able to resolve 6 to 9 bands between 300 and 2000 kilobases (kb), distributed in 16-22 large and up to 31 small chromosomes for E. histolytica DNA. For E. invadens, 4 to 5 bands between 300 and over 2000 kb were resolved and discriminated in 4 large and 6 small chromosomes. A ribosomal probe from Trypanosoma brucei hybridized with a 1100 kb band in E. histolytica strain HM1:IMSS and with a 1000 kb band in clone A of strain HM1:IMSS. Both cell lines also showed hybridization at the origin. The ribosomal probe hybridized only at the origin of the E. invadens DNA lane. A triosephosphate isomerase DNA probe from T. brucei hybridized only with a 2000 kb band in E. invadens, indicating that banding patterns are chromosomal bands and ruling out the possibility of DNA degradation.
Valdés
J
J
Department of Genetics and Molecular Biology, CINVESTAV I.P.N., México, D.F.
de la Cruz Hernández
F
F
Ocádiz
R
R
Orozco
E
E
D000818:Animals; D015139:Blotting, Southern; D002875:Chromosomes / Q000737:chemistry; D016054:DNA, Protozoan / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D004747:Entamoeba / Q000235:genetics*; D004748:Entamoeba histolytica / Q000235:genetics; D007621:Karyotyping; D009693:Nucleic Acid Hybridization
D016054:DNA, Protozoan