A biochemical investigation of auranofin nephrotoxicity by high field proton nuclear magnetic resonance (NMR) spectroscopy.
High-field proton NMR spectroscopic analysis of urine and plasma has been employed to study the biochemical effects and nephrotoxic action of an intramuscular dose of auranofin in rats. Auranofin induced a characteristic profile of proximal tubular damage as evidenced by aminoaciduria, lactic aciduria and increased urinary acetate concentrations. In addition, ethanol was detectable in both urine and plasma obtained from auranofin-treated rats. Auranofin-mediated elevations in the plasma and urine concentrations of 3-D-hydroxybutyrate indicated an increased utilisation of fats for fuel in rats treated with this novel therapeutic agent.
Grootveld
M
M
Inflammation Group, London Hospital Medical College, U.K.
Claxson
A W
AW
Naughton
D
D
Blake
D R
DR
D000085:Acetates / Q000097:blood; D000426:Alcohol Dehydrogenase / Q000037:antagonists & inhibitors; D000596:Amino Acids / Q000652:urine; D000818:Animals; D001310:Auranofin; D006885:Hydroxybutyrates / Q000097:blood; D007656:Ketoglutaric Acids / Q000097:blood; D007674:Kidney Diseases / Q000139:chemically induced* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains
D000085:Acetates; D000596:Amino Acids; D006885:Hydroxybutyrates; D007656:Ketoglutaric Acids; D001310:Auranofin; D000426:Alcohol Dehydrogenase