Characterization of estrogen receptor in human gastric cancer.
Estrogen receptors (ER) were examined in cytosol, nuclear potassium chloride (KCl) extractable fraction, and nuclear KCl unextractable fraction by the dextran-coated charcoal adsorption method in various gastric cancer tissue. The overall ER-positive rate in the cytosol and nuclear fraction was 19.2%. The maximum binding site (Bmax) was 36.0 to 175.0 fmol/mg of protein, and the dissociation constant (Kd) was 0.6 to 1.6 X 10(-9) in cytosol fraction. In the nuclear fraction, Bmax was 7.5 fmol/mg of DNA and Kd was 2.3 X 10(-9). Estrogen receptors were characterized in cytosol protein. In cytosol, the estrogen (E2)-ER complex was sedimented at approximately the 5S and 8S regions by 5% to 20% linear sucrose gradient centrifugation. A steroid specificity study of ER showed the presence of an binder in gastric cancer tissue. In conclusion, these results that gastric cancer tissue has E2 binding sites with the same biochemical characteristics as in breast cancer and endometrial cancer strongly suggest the hormonal dependency of gastric cancer.
Matsui
M
M
First Department of Surgery, Kyoto Prefectural University of Medicine, Japan.
Kojima
O
O
Uehara
Y
Y
Takahashi
T
T
D000230:Adenocarcinoma / Q000737:chemistry*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011869:Radioligand Assay; D011960:Receptors, Estrogen / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013274:Stomach Neoplasms / Q000737:chemistry*; D013347:Subcellular Fractions / Q000737:chemistry
D011960:Receptors, Estrogen