[Catalytic activity of steroid conjugates of various types of peroxidases in aqueous and micellar media].
The horseradish peroxidase (HRP) conjugates with the sheep antirabbit antibodies and cortisol (COR) or progesterone (PROG) containing 9 to 40 steroid molecules per HRP molecule were synthesized. In aqueous media all the conjugates have lower catalytic activity in the o-phenylenediamine oxidation than the native enzyme. In reversed Aerosol OT micelles in heptane the HRP-COR and HRP-PROG conjugates containing 12 and 9 steroid molecules, respectively, have catalytic constants 2.6 and 2.7 times higher than the unmodified enzyme. The influence of the HRP hydrophobisation and its inactivation on the course of modification on catalytic properties of the enzyme is discussed.
Eremin
A N
AN
Petrusha
O G
OG
Matveentsev
V D
VD
Metelitsa
D I
DI
D002384:Catalysis; D006735:Horseradish Peroxidase / Q000737:chemistry* / Q000276:immunology; D006854:Hydrocortisone / Q000737:chemistry* / Q000276:immunology; D007700:Kinetics; D008823:Micelles; D010084:Oxidation-Reduction; D011374:Progesterone / Q000737:chemistry* / Q000276:immunology; D014867:Water
D008823:Micelles; D014867:Water; D011374:Progesterone; D006735:Horseradish Peroxidase; D006854:Hydrocortisone
Kataliticheskaia aktivnots' steroidnykh kon''iugatov peroksidazy raznogo sostava v vodnykh i mitselliarnykh sredakh.