Liquid chromatography with amperometric detection for the determination of non-steroidal anti-inflammatory drugs in plasma.
A simple and sensitive liquid chromatographic method with electrochemical detection is described for the quantitative determination of the non-steroidal anti-inflammatory drugs diflunisal, indomethacin, naproxen, piroxicam and sulindac in human plasma. Isolation of the drug from the biological fluid is achieved using a Sep-pak RP18 cartridge. Separation of plasma components occurs on a reversed-phase C18 column with a mobile phase consisting of methanol-water-phosphate buffer. For the amperometric detection the potential of +0.9 V was set on the working electrode. The detection limit of the assay is 10-20 ng/ml. The method showed good concordance for plasma samples containing the drugs (r = 0.999) and can be readily utilized for clinical pharmacokinetic studies.
Kazemifard
A G
AG
Department of Pharmacy, University of Sydney, Australia.
Moore
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DE
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D004061:Diflunisal / Q000097:blood; D004553:Electric Conductivity; D006801:Humans; D007213:Indomethacin / Q000097:blood; D009288:Naproxen / Q000097:blood; D010894:Piroxicam / Q000097:blood; D013467:Sulindac / Q000097:blood
D000894:Anti-Inflammatory Agents, Non-Steroidal; D010894:Piroxicam; D013467:Sulindac; D009288:Naproxen; D004061:Diflunisal; D007213:Indomethacin