Hemoglobin-acetaldehyde adducts in human volunteers following acute ethanol ingestion.
Rabbit antibodies against albumin-acetaldehyde adduct were used in an enzyme-linked immunosorbent assay to detect acetaldehyde-hemoglobin condensates from the blood of 12 volunteers following ingestion of 1.3 to 2.9 g of ethanol per kg body weight during 8 hr. Blood samples were drawn before drinking and between 2 to 46 hr after starting the drinking session. While there were no significant increases in blood acetaldehyde levels in these samples, acetaldehyde-hemoglobin adducts were significantly increased in the samples drawn after ethanol had been eliminated from the body. Administration of ethanol (0.1 g/kg) to an Oriental flusher resulted in an increase both in blood acetaldehyde and the hemoglobin-acetaldehyde adduct levels. These results suggest that acetaldehyde-hemoglobin condensates are formed in vivo following acute ethanol ingestion. Such condensates may be of value to mark alcohol consumption.
Niemelä
O
O
Department of Clinical Chemistry, University of Oulu, Finland.
Israel
Y
Y
Mizoi
Y
Y
Fukunaga
T
T
Eriksson
C J
CJ
D000079:Acetaldehyde / Q000493:pharmacokinetics*; D000328:Adult; D000435:Alcoholic Intoxication / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005483:Flushing / Q000097:blood; D006441:Hemoglobin A / Q000378:metabolism*; D006801:Humans; D008297:Male; D011485:Protein Binding / Q000502:physiology
D006441:Hemoglobin A; D000079:Acetaldehyde