Induction of specific-locus and dominant lethal mutations in male mice by busulfan.
The chemotherapeutic agent busulfan was tested for the induction of dominant lethal and specific-locus mutations in male mice. A dose of 5 mg/kg b.w. of busulfan induces dominant lethal mutations in spermatozoa. A dose of 20 mg/kg b.w. induces dominant lethal mutations in spermatozoa and spermatids. A total of 83,196 offspring were scored in the specific-locus experiments. Busulfan-induced specific-locus mutations were recovered in spermatozoa and spermatids, but not in spermatogonia. The sensitivity patterns for the induction of dominant lethal and specific-locus mutations by busulfan in germ cells of male mice are similar but not identical.
Ehling
U H
UH
GSF-Institut für Säugetiergenetik, Neuherberg, F.R.G.
Neuhäuser-Klaus
A
A
D000483:Alleles; D000818:Animals; D002066:Busulfan / Q000633:toxicity*; D005260:Female; D005799:Genes, Dominant; D005804:Genes, Lethal; D006720:Homozygote; D008297:Male; D051379:Mice; D016296:Mutagenesis; D013087:Spermatids / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects*
D002066:Busulfan