Inhibitory effects of phospholipase D on chondrogenesis in vitro.
Mesenchymal cells from the wing buds of stage 24 chick embryos undergo differentiation to cartilage when plated at high density. Treatment of these cultures with phospholipase D resulted in inhibition of chondrogenesis. Phospholipase D treatment (which produces phosphatidic acid from membrane phospholipids) was found to affect cell proliferation and to dramatically increase intracellular free calcium levels and inositol phosphate production. Intracellular free Ca2+, mobilized as a result of phosphatidylinositol phosphate hydrolysis, may therefore inhibit chondrogenesis in embryonic mesenchymal cells.
Tsonis
P A
PA
Department of Biology, University of Dayton, Ohio 45469-2320.
Del Rio-Tsonis
K
K
Rothrock
J
J
Dominguez
J
J
English
D
D
Glade
K
K
Goetinck
P F
PF
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002356:Cartilage / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D007295:Inositol Phosphates / Q000378:metabolism; D010739:Phospholipase D / Q000494:pharmacology*
D007295:Inositol Phosphates; D010739:Phospholipase D; D002118:Calcium