title: Simulcast: contiguous views of fracture faces and membrane surfaces in a single cell.
abstract: We introduce "simulcast", a new method that combines the advantages of freeze-fracture with those of "fracture-flip" (Anderson-Forsman, C., P. Pinto da Silva, J. Cell Sci. 90, 531-541 (1988)) to provide images of the fracture faces and membrane surfaces of the same membrane in a single cell. The method involves low-angle unidirectional shadowing, careful azimuthal reorientation of the replicas, flipping, and re-shadowing. Simulcast relates, in a single image, the freeze-fracture morphology of fracture faces, the nanoanatomy of membrane surfaces and the topochemistry of surface receptors/antigens.
authors.affiliation: Membrane Biology Section, National Cancer Institute, Frederick Cancer Research MD 21702-1201.
authors.forename: S
authors.initials: S
authors.lastname: Ru-Long
authors.forename: P
authors.initials: P
authors.lastname: Pinto da Silva
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure*; D005614:Freeze Fracturing / Q000379:methods*; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D012617:Sea Urchins; D013094:Spermatozoa / Q000648:ultrastructure