The complete amino acid sequence of toxin TsTX-VI isolated from the venom of the scorpion Tityus serrulatus.
The complete sequence of the toxin TsTX-VI from the venom of the scorpion Tityus serrulatus Lutz and Mello is presented. The sequence has been determined by automated Edman analysis of the reduced and carboxymethylated protein as well as of the resulting peptides, obtained from S. aureus protease and tryptic digestions. TsTX-VI is composed of 62 residues and has a calculated molecular weight of 6717. Homology studies with other scorpion toxins show that TsTX-VI is more similar to the Old World than to the North American scorpion toxins. The hydropathic index indicates that TsTX-VI is more hydrophobic than Ts-gamma. Toxicity studies carried out in mice demonstrate that i.v. injection of TsTX-VI is unable to evoke the usual symptoms induced by the typical neurotoxins of this venom, but only a generalized allergic reaction. These properties are important in clarifying the relationship between primary structure and biological function of scorpion toxins.
Marangoni
S
S
Department of Pathology, New York University Medical Center, New York 10016.
Ghiso
J
J
Sampaio
S V
SV
Arantes
E C
EC
Giglio
J R
JR
Oliveira
B
B
Frangione
B
B
D000595:Amino Acid Sequence; D000818:Animals; D010450:Endopeptidases; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000737:chemistry*; D010446:Peptide Fragments / Q000302:isolation & purification; D012604:Scorpion Venoms / Q000032:analysis* / Q000737:chemistry*; D012689:Sequence Homology, Nucleic Acid; D013329:Structure-Activity Relationship; D014357:Trypsin
D009498:Neurotoxins; D010446:Peptide Fragments; D012604:Scorpion Venoms; C068452:toxin TsTX-VI; D010450:Endopeptidases; D014357:Trypsin