title: [Separation using RP-HPLC of non-human globin in the study of hemoglobin polymorphism].
abstract: The successful extension of the reversed-phase HPLC methodology to the study of non-human globin chains is shown. A large-pore C4 column, and simple acetonitrile in aqueous TFA gradients, were used to completely separate and quantitate globin chains from caprines, swines, rodents, and birds. Results indicate a large hemoglobin polymorphism in some species.
authors.affiliation: Istituto di Fisiologia Generale e Chimica Biologica, Università di Sassari.
authors.forename: L
authors.initials: L
authors.lastname: Manca
authors.forename: E
authors.initials: E
authors.lastname: Cocco
authors.forename: S
authors.initials: S
authors.lastname: Naitana
authors.forename: B
authors.initials: B
authors.lastname: Masala
mesh_terms: D000818:Animals; D001717:Birds / Q000097:blood* / Q000235:genetics; D002851:Chromatography, High Pressure Liquid; D005914:Globins / Q000235:genetics / Q000302:isolation & purification*; D008322:Mammals / Q000097:blood* / Q000235:genetics; D011110:Polymorphism, Genetic; D013045:Species Specificity
chemical_list: D005914:Globins
vernacular_title: Separazione mediante RP-HPLC di globine non umane nello studio del polimorfismo della emoglobina.