High-performance liquid chromatographic method for the study of solvent effects on the peptidase and esterase activity of thermitase.
The high-performance liquid chromatographic separation of enzymatic degradation products of a multi-functional peptide substrate under isocratic conditions is described. This technique was applied to the study of solvent effects on the peptidase and esterase activity of thermitase.
Dudek
S
S
Institute of Biochemistry, Medical Faculty, Martin-Luther University, Halle G.D.R.
Friebe
S
S
Hermann
P
P
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010450:Endopeptidases / Q000378:metabolism*; D004950:Esterases / Q000378:metabolism*; D012697:Serine Endopeptidases; D012997:Solvents / Q000494:pharmacology*
D012997:Solvents; D004950:Esterases; D010450:Endopeptidases; D012697:Serine Endopeptidases; C022728:thermitase