title: [Experimental study of a model for granulocyte storage].
abstract: An efficient granulocytes preservation would allow an easier study of their structure and functions, of granulocytes functional diseases (e.g. chronic granulomatous disease) and an easier use of granulocytes as a clinical tool (e.g. for localizing infections or for transfusions). Unfortunately, granulocytes are nearly impossible to preserve, even in a short-term storage (less than 24 hours). This study proposes an experimental model which could improve in vitro granulocyte functions maintenance. Bicarbonate buffer, glucose, adenosine triphosphate, vitamins C and E, nicotinamide adenine dinucleotide, nicotinamide adenine dinucleotide phosphate, amikacin, and ampicillin supplementation significantly (p 0.05) improve maintenance of one or several granulocyte functions during storage.
authors.affiliation: Centre régional de transfusion sanguine de Nancy-Brabois, Vandceuvre-les-Nancy.
authors.forename: L
authors.initials: L
authors.lastname: Verstraeten
authors.forename: M
authors.initials: M
authors.lastname: Marchand-Arvier
authors.forename: F
authors.initials: F
authors.lastname: Schooneman
authors.forename: C
authors.initials: C
authors.lastname: Vigneron
mesh_terms: D002634:Chemotaxis, Leukocyte / Q000502:physiology; D006098:Granulocytes / Q000502:physiology; D006801:Humans; D011309:Preservation, Biological / Q000379:methods*
vernacular_title: Etude expérimentale d'un modèle de conservation des polynucléaires neutrophiles.