[Uracil, contained in DNA, can direct the correction of mispaired nucleotides in human cells].
The mismatch correction has been studied in human cells and presented in this paper. In the study the experimental model with half-containing hetroduplex (-1 residue in the polylinker region of lac gene) M13 DNA has been used. M 13 DNA was isolated from human cells 24 hours after transfection and transformed into ung+ and ung- cells of Escherichia coli. The percentage of lac- colonies (formed due to frameshift mutation in the lac gene) was analyzed. The increased percent of lac- mutations after human transfection indicated that DNA-uracil can polarize mismatch correction in human cells.
Golubovskaia
V M
VM
Aprelikova
O N
ON
Tomilin
N V
NV
D001435:Bacteriophages / Q000235:genetics; D001482:Base Composition; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005814:Genes, Viral; D006801:Humans; D009154:Mutation; D009692:Nucleic Acid Heteroduplexes; D009711:Nucleotides / Q000235:genetics*; D014162:Transfection; D014498:Uracil / Q000378:metabolism*
D009692:Nucleic Acid Heteroduplexes; D009711:Nucleotides; D014498:Uracil; D004247:DNA
Uratsil, soderzhashchiĭsia v DNK, mozhet napravliat' koretsiiu nesparennykh nukleotidov v kletkakh cheloveka.