title: Rapid and standardized quantitation of hemolytic activity of the fourth component of human complement.
abstract: Based on a method that uses the fourth component of complement (C4)-deficient guinea pig serum to quantify the hemolytic activity of C4, we developed an assay that allows the processing of a large number of individual samples with high reproducibility. In contrast to the conventional procedure using titration curves of each sample to be determined, we can show that a single appropriate dilution of the sample allows accurate quantitation of hemolytic activity. The reliability of the procedure is demonstrated using either C4A- or C4B- deficient and normal individual samples.
authors.affiliation: Institut für Rechstmedizin, Johannes-Gutenberg-Universität Mainz, FRG.
authors.forename: T
authors.initials: T
authors.lastname: Kaufmann
authors.forename: H J
authors.initials: HJ
authors.lastname: Kytzia
authors.forename: C
authors.initials: C
authors.lastname: Rittner
mesh_terms: D003181:Complement C4 / Q000235:genetics / Q000276:immunology*; D003171:Complement Pathway, Classical; D004912:Erythrocytes / Q000276:immunology; D006461:Hemolysis; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D011110:Polymorphism, Genetic
chemical_list: D003181:Complement C4