Post-column detection by on-line photolysis-electrochemistry in liquid chromatography; application to the determination of phenylalanine in human urine.
On-line, post-column photochemical derivatization for liquid chromatography-electrochemical detection (LC-hv-EC) has been employed for the analysis of phenylalanine in urine. The method is highly sensitive and selective. The quantitative results show good precision and reproducibility. It can be used easily and has the clinical use of diagnosing phenylketonuria disease.
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Department of Chemistry, Barnett Institute, Northeastern University, Boston, MA 02115.
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D002853:Chromatography, Liquid; D004563:Electrochemistry; D006801:Humans; D007202:Indicators and Reagents; D010649:Phenylalanine / Q000652:urine*; D010777:Photochemistry
D007202:Indicators and Reagents; D010649:Phenylalanine