[Identification of myosin light chain 1 from human heart ventricles on two-dimensional electrophoretograms using monoclonal antibodies].
Two-dimensional electrophoresis performed according to O'Farrell was used to isolate the myosin light chain I from human heart ventricles. This chain was further used to obtain hybridomas able to secrete monoclonal antibodies. This method permits to increase the yield of monoclonal antibody-secreting hydridomas and to spare effort with regard to cultivation and testing of experimental samples.
Kovalev
L I
LI
Volgin
A Iu
AIu
Konorova
A L
AL
Tsvetkova
M N
MN
Puliaeva
E V
EV
Pevnitskiĭ
L A
LA
Shishkin
S S
SS
D000911:Antibodies, Monoclonal / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D006352:Heart Ventricles / Q000737:chemistry*; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D009218:Myosins / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D009218:Myosins
Identifikatsiia legkoĭ tsepi 1 miozina iz zheludochkov serdtsa cheloveka na dvumernykh élektroforegrammakh s pomoshch'iu monoklonal'nykh antitel.