Evidence that the protein components of bovine erythrocyte green heme binding protein and flavin reductase are identical.
Bovine erythrocyte green heme binding protein and bovine erythrocyte flavin reductase have been isolated in highly purified forms and subjected to amino acid analysis and N-terminal amino acid sequence analysis. The two proteins possess similar amino acid compositions and identical N-terminal amino acid sequences. Moreover, the two proteins are immunochemically cross-reactive and are indistinguishable when compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and by double diffusion technique. This study provides evidence that the protein components of bovine erythrocyte green heme binding protein and flavin reductase are identical.
Quandt
K S
KS
Department of Biological Chemistry, Medical School, University of Michigan, Ann Arbor 48109-0606.
Xu
F
F
Chen
P
P
Hultquist
D E
DE
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D042966:Cytochrome-B(5) Reductase / Q000097:blood* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism*; D007106:Immune Sera; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid
D007106:Immune Sera; D010446:Peptide Fragments; D042966:Cytochrome-B(5) Reductase