Studies concerning ATP dynamics in essential hypertension.
The use of Beckmann LS-7000 liquid scintillation spectrometer enabled us to develop a rapid and reproducible technique for the enzymatic microdosage of ATP by means of the bioluminescence reaction of the luciferin-luciferase system from the firefly. Changes of ATP concentration in the plasma, erythrocytes and cerebrospinal fluid of the hypertensive subjects as compared to the control ones have been revealed by means of this method. According to our results ATP concentration significantly increases in the plasma and cerebrospinal fluid and slightly modifies in the erythrocytes of the hypertensive as compared with control subjects. Correlation of data concerning the variation of ATP distribution in the different compartments (plasma, erythrocytes, cerebrospinal fluid) with those related to Na and K content in hypertensive and control subjects seems to be useful for some remarks on the molecular mechanisms in the essential hypertension.
Vasilescu
V
V
Department of Biophysics, Faculty of Medicine, Bucharest, Romania.
Tripşa
M F
MF
Dinu
A
A
D000255:Adenosine Triphosphate / Q000032:analysis*; D000328:Adult; D002555:Cerebrospinal Fluid / Q000737:chemistry; D004912:Erythrocytes / Q000737:chemistry; D006801:Humans; D006973:Hypertension / Q000378:metabolism*; D008875:Middle Aged; D010949:Plasma / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D000255:Adenosine Triphosphate