Evidence that the M antigen of Histoplasma capsulatum var. capsulatum is a catalase which exhibits cross-reactivity with other dimorphic fungi.
A panel of monoclonal antibodies (Mabs) was raised against histoplasmin, the antigen derived from the mycelial phase of Histoplasma capsulatum var. capsulatum which contains the diagnostically useful H and M antigens. A number of Mabs were obtained which recognized a 70-75 kD component of an antigenic preparation of H. capsulatum var. capsulatum by Western blotting. When reacted with histoplasmin by Western blotting the Mabs recognized a similar 70-75 kD band, together with a series of higher molecular mass bands at approximately 130, 190 and 230 kD, a pattern which correlates strongly with both the published relative molecular mass (Mr) of the M antigen and the known subunit structure of the enzyme catalase. These Mabs were also shown to recognize a commercial preparation of Aspergillus niger catalase by ELISA. Other dimorphic fungi were also reactive with these Mabs by Western blotting, indicating the presence of common epitopes on the catalase molecules of these species.
Hamilton
A J
AJ
Department of Clinical Sciences, Guys Hospital, London Bridge, UK.
Bartholomew
M A
MA
Figueroa
J
J
Fenelon
L E
LE
Hay
R J
RJ
D000911:Antibodies, Monoclonal / Q000276:immunology; D000946:Antigens, Fungal / Q000737:chemistry* / Q000276:immunology; D015153:Blotting, Western; D002374:Catalase / Q000737:chemistry* / Q000276:immunology; D003429:Cross Reactions; D006658:Histoplasma / Q000201:enzymology / Q000276:immunology*; D006659:Histoplasmin / Q000276:immunology
D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal; D006659:Histoplasmin; D002374:Catalase