title: A biochemical investigation of auranofin nephrotoxicity by high field proton nuclear magnetic resonance (NMR) spectroscopy.
abstract: High-field proton NMR spectroscopic analysis of urine and plasma has been employed to study the biochemical effects and nephrotoxic action of an intramuscular dose of auranofin in rats. Auranofin induced a characteristic profile of proximal tubular damage as evidenced by aminoaciduria, lactic aciduria and increased urinary acetate concentrations. In addition, ethanol was detectable in both urine and plasma obtained from auranofin-treated rats. Auranofin-mediated elevations in the plasma and urine concentrations of 3-D-hydroxybutyrate indicated an increased utilisation of fats for fuel in rats treated with this novel therapeutic agent.
authors.affiliation: Inflammation Group, London Hospital Medical College, U.K.
authors.forename: M
authors.initials: M
authors.lastname: Grootveld
authors.forename: A W
authors.initials: AW
authors.lastname: Claxson
authors.forename: D
authors.initials: D
authors.lastname: Naughton
authors.forename: D R
authors.initials: DR
authors.lastname: Blake
mesh_terms: D000085:Acetates / Q000097:blood; D000426:Alcohol Dehydrogenase / Q000037:antagonists & inhibitors; D000596:Amino Acids / Q000652:urine; D000818:Animals; D001310:Auranofin; D006885:Hydroxybutyrates / Q000097:blood; D007656:Ketoglutaric Acids / Q000097:blood; D007674:Kidney Diseases / Q000139:chemically induced* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000085:Acetates; D000596:Amino Acids; D006885:Hydroxybutyrates; D007656:Ketoglutaric Acids; D001310:Auranofin; D000426:Alcohol Dehydrogenase