title: Heterologous gene expression on the linear DNA killer plasmid from Kluyveromyces lactis.
abstract: Linear hybrid plasmids based on the killer plasmid pGKL1 from Kluyveromyces lactis were obtained by in vivo recombination in Saccharomyces cerevisiae. Like pGKL1, the hybrids are located in the cytoplasm, have terminal inverted repeats (TIR) and possess covalently linked proteins at their 5' ends. The construction of cytoplasmic hybrid plasmids is based on the use of a pGKL1 promoter to control the marker gene used for recombination. Nuclear promoters are not recognised in the cytoplasm.
authors.affiliation: Lehrstuhl für Allgemeine Botanik, Ruhr-Universität Bochum, Federal Republic of Germany.
authors.forename: J
authors.initials: J
authors.lastname: Kämper
authors.forename: K
authors.initials: K
authors.lastname: Esser
authors.forename: N
authors.initials: N
authors.lastname: Gunge
authors.forename: F
authors.initials: F
authors.lastname: Meinhardt
mesh_terms: D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004271:DNA, Fungal; D015870:Gene Expression; D007716:Kluyveromyces / Q000235:genetics*; D008969:Molecular Sequence Data; D010641:Phenotype; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014170:Transformation, Genetic
chemical_list: D004271:DNA, Fungal