title: 6-Deoxy-6-bromo-ascorbic acid inhibits growth of mouse melanoma cells.
abstract: The influence of 6-deoxy-6-bromo-ascorbic acid (6-Br-AA) and ascorbic acid (AA) on the growth of mouse melanoma cell line B16, mouse fibroblasts (L929), and human cervical carcinoma HeLa cells was examined by in vitro experiments. 6-Br-AA was added in concentrations 10(-1) to 10(-8)M and for incubation periods of 2, 18, 24 and 72 h. The present results indicate that 6-Br-AA exhibits a highly pronounced inhibiting effect on growth and DNA synthesis of melanoma cells. Inhibitory effect of 6-Br-AA was confirmed by the in vivo experiments. The tumor-suppressing effect on solid melanoma B16 was attained with 9 mg per mouse given three times daily for 16 days. Our preliminary results indicate that 6-Br-AA could serve as a potential antitumor agent.
authors.affiliation: Ruder Bosković Institute, Zagreb, Yugoslavia.
authors.forename: M
authors.initials: M
authors.lastname: Osmak
authors.forename: M
authors.initials: M
authors.lastname: Eckert-Maksić
authors.forename: K
authors.initials: K
authors.lastname: Pavelić
authors.forename: Z B
authors.initials: ZB
authors.lastname: Maksić
authors.forename: R
authors.initials: R
authors.lastname: Spavénti
authors.forename: L
authors.initials: L
authors.lastname: Beketić
authors.forename: I
authors.initials: I
authors.lastname: Kovacek
authors.forename: B
authors.initials: B
authors.lastname: Susković
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D008297:Male; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D000970:Antineoplastic Agents; C068572:6-deoxy-6-bromoascorbic acid; D001205:Ascorbic Acid