title: Evaluation of Setaria cervi antigen in the diagnosis of human filariasis.
abstract: Bovine filariid worm (Setaria cervi) antigen was evaluated for the immunodiagnosis of human filariasis. Patients with manifestations of filarial infection; [1) microfilaremia cases, (2) chronic clinical cases, (3) tropical pulmonary eosinophilia (TPE) cases) were taken for the investigations along with (4) normal endemic and non-endemic human subjects. CIEP, IFAT and ELISA tests were employed for detection of serum antibodies. There was a high degree of sensitivity shown by IFAT (1:64-1:256) and ELISA (1:200-1:20,800) in microfilaraemia cases and higher in chronic clinical cases with IFAT (1:128-1:1,024) and ELISA (1:12,800-1:102,400). Only one TPE case showed positive titre by IFAT (1:64) and ELISA (1:200) whereas sera from controls and patients with helminth infections did not show positive titres (1:100). In the case of CIEP, positive reaction was seen in only one case from group I.
authors.affiliation: Department of Zoonosis, Haffkine Institute, Parel, Bombay, India.
authors.forename: A J
authors.initials: AJ
authors.lastname: Almeida
authors.forename: M K
authors.initials: MK
authors.lastname: Bhopale
authors.forename: D M
authors.initials: DM
authors.lastname: Renapurkar
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth; D003378:Counterimmunoelectrophoresis; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005368:Filariasis / Q000175:diagnosis*; D005370:Filarioidea / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D008875:Middle Aged; D012719:Setariasis
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007136:Immunoglobulins