title: Preparative scale isolation of sphingosine.
abstract: A high-yield method is described for the preparation of sphingenine from galactosylceramide. Two successive cleavage reactions are used, alkaline butanol to form galactosylsphingosine, then acidic acetonitrile to form the sphingenine. A column purification procedure utilizing silica gel is described. The method is superior to previously described methods in its rapidity and avoidance of isomerization and contamination by racemization and rearrangement products.
authors.affiliation: Mental Health Research Institute, University of Michigan, Ann Arbor 48109.
authors.forename: N S
authors.initials: NS
authors.lastname: Radin
mesh_terms: D000097:Acetonitriles; D000440:Butanols; D002855:Chromatography, Thin Layer / Q000379:methods*; D005699:Galactosylceramides / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D013110:Sphingosine / Q000302:isolation & purification*
chemical_list: D000097:Acetonitriles; D000440:Butanols; D005699:Galactosylceramides; D013110:Sphingosine