Analysis of caprine mycoplasmas and mycoplasma infections in goats using two-dimensional electrophoresis and immunoblotting.
Seven strains of mycoplasma have been characterized by two-dimensional polyacrylamide gel electrophoresis in combination with immunoblotting. The two-dimensional patterns of the strains were compared and similarities and differences are discussed. Hyperimmune rabbit antisera were used in the immunoblotting experiment to study the immunological cross-reactivity between the species. Sera from goats, naturally and experimentally infected with mycoplasma, were also used in the immunoblotting experiment to study the immune responses of the infected animals.
Olsson
B
B
Pharmacia AB, Uppsala, Sweden.
Bölske
G
G
Bergström
K
K
Johansson
K E
KE
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000276:immunology; D003429:Cross Reactions; D015180:Electrophoresis, Gel, Two-Dimensional; D015511:Goat Diseases / Q000276:immunology*; D006041:Goats; D009174:Mycoplasma / Q000276:immunology*; D009175:Mycoplasma Infections / Q000276:immunology / Q000662:veterinary*
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins