Protein kinase C (alpha and beta) immunoreactivity in rabbit and rat retina: effect of phorbol esters and transmitter agonists on immunoreactivity and the translocation of the enzyme from cytosolic to membrane compartments.
Using a monoclonal antibody against protein kinase C (PKC) that recognises the isoenzymes alpha, beta I, and beta II, positive immunoreactivity was observed throughout the cytoplasm of bipolar cells in both rat and rabbit retinas. PKC immunoreactivity was also associated with the outer segment of photoreceptors in the rabbit retina and presumed amacrine cells in the rat retina. The PKC immunoreactivity in the retina was unaffected in content or localisation in rats kept in continuous dark or light conditions over a period of 6 days. The localisation of PKC immunoreactivity in retinas was similar in 6-day-old, 16 day-old, or adult rabbits. However, the content of PKC was lowest at the youngest stage and highest in the adult rabbit retinas. Of the two active phorbol esters studied, only phorbol 12,13-dibutyrate (PDbut) at a concentration of 1 microM caused the PKC immunoreactivity in rabbit retina bipolar cells to be "transported" from the perikarya towards the axonal terminal processes. Biochemical analyses showed that most of the cytosolic PKC was translocated to the membrane compartment following such treatment. The other phorbol ester, phorbol 12-myristate 13-acetate, even at a concentration of 10 microM did not cause a similar transport of PKC immunoreactivity in the bipolar cells, although a partial translocation of the enzyme could be followed biochemically. Both the translocation and transport of PKC by PDbut could be reversed by simply incubating the retinas in physiological solution for 60 min. The "transport" and translocation processes were not obviously affected by the transport inhibitor colchicine or by known PKC inhibitor such as staurosporine, H-7, sphingosine, or polymyxin B. In addition, agonists known to stimulate inositol phosphates in the retina, viz., carbachol, noradrenaline, and quisqualate, or 4-aminopyridine did not cause a translocation or "transport" of PKC as observed for the phorbol esters.
Osborne
N N
NN
Nuffield Laboratory of Ophthalmology, Oxford University, England.
Broyden
N J
NJ
Barnett
N L
NL
Morris
N J
NJ
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D015761:4-Aminopyridine / Q000494:pharmacology; D000375:Aging; D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D000911:Antibodies, Monoclonal; D002462:Cell Membrane / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D005455:Fluorescent Antibody Technique; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D007546:Isoquinolines / Q000494:pharmacology; D007700:Kinetics; D010703:Phorbol Esters / Q000494:pharmacology*; D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D016318:Quisqualic Acid / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D012160:Retina / Q000201:enzymology* / Q000254:growth & development; D013110:Sphingosine / Q000494:pharmacology; D019311:Staurosporine
D000470:Alkaloids; D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D007546:Isoquinolines; D010703:Phorbol Esters; D010879:Piperazines; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D016318:Quisqualic Acid; D015761:4-Aminopyridine; D011493:Protein Kinase C; D019311:Staurosporine; D013110:Sphingosine