D11S146 and BCL1 are physically linked but can be discriminated by their amplification status in human breast cancer.
Band q13 of chromosome 11 is frequently altered in a number of human cancers. We have undertaken physical mapping in this region, starting with D11S146, an anonymous 11q13 DNA fragment. This probe has been used by others as a landmark to locate MEN1, a locus of predisposition to multiple endocrine neoplasia. Long-range restriction mapping locates D11S146 within approximately 400 kb of the BCL1 translocation breakpoint involved in certain B-cell malignancies. BCL1 and two proto-oncogenes, INT2 and HST, were previously found to be coamplified in approximately 1/5 breast carcinomas. Although close to BCL1, D11S146 is present in less than 3/4 of these amplification units and delimits their centromeric boundary. Therefore, we propose that D11S146 defines two genetic regions. The centromeric region--PYGM/D11S146--contains MEN1. The telomeric one includes the D11S146/BCL1/INT2/HST area and is relevant to DNA amplification in carcinomas and to B-cell translocations.
Szepetowski
P
P
LGMCH, UFR de Médecine, Nice, France.
Nguyen
C
C
Perucca-Lostanlen
D
D
Carle
G F
GF
Tsujimoto
Y
Y
Birnbaum
D
D
Theillet
C
C
Gaudray
P
P
D001943:Breast Neoplasms / Q000235:genetics*; D002294:Carcinoma, Squamous Cell; D002880:Chromosomes, Human, Pair 11; D015342:DNA Probes; D004273:DNA, Neoplasm; D005784:Gene Amplification; D008040:Genetic Linkage; D006801:Humans; D015183:Restriction Mapping; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D004273:DNA, Neoplasm