Purification of transferrins and lactoferrin using DEAE affi-gel blue.
1. A simple method for purifying transferrins and lactoferrin is described. 2. The method consists of a preliminary step of dye-ligand chromatography using DEAE Affi-Gel Blue as the gel matrix at pH 7.5. In this chromatographic step, the transferrins and lactoferrin were readily separated from the bulk of the other proteins by start buffer elution. 3. Differences in the chromatographic behaviour of the various serum transferrins (monkey, human, rabbit, pig, chicken and duck) and ovotransferrin upon DEAE Affi-Gel Blue chromatography can be attributed to differences in the anionic charge of the transferrins in 0.02 M potassium phosphate buffer, pH 7.5, thus resulting in the differential retardation of these protein molecules by the gel matrix. 4. The result of DEAE Affi-Gel Blue chromatography of human lactoferrin is different from that for the transferrins. This may possibly reflect the differences in the strength of interaction between lactoferrin and transferrin with this gel matrix.
Chung
M C
MC
Department of Biochemistry, National University of Singapore.
Chan
S L
SL
Shimizu
S
S
D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D002850:Chromatography, Gel; D004372:Ducks; D006801:Humans; D007781:Lactoferrin / Q000302:isolation & purification*; D008252:Macaca fascicularis; D008969:Molecular Sequence Data; D011817:Rabbits; D013552:Swine; D014168:Transferrin / Q000737:chemistry / Q000302:isolation & purification*; D014227:Triazines
D014168:Transferrin; D014227:Triazines; C038830:DEAE affigel blue; D007781:Lactoferrin