Low stimulation of NADH oxidation and oxygen consumption by 5-iminodaunorubicin and its derivatives.
NADH oxidation and oxygen consumption mediated by 5-iminodaunorubicin and its derivatives have been studied. Experiments were carried out using two enzyme system: microsomes isolated from rat liver and cytochrome c-reductase. All 5-iminoanthracyclines examined were relatively poor electron transfer mediators. In addition, 5-imino-derivatives of daunorubicin modified at sugar moiety were less effective in stimulating NADH oxidation and oxygen radical production than 5-iminodaunorubicin itself. This may be due to the additive effect of 5-imino structure and the presence of bulky substituents at sugar moiety, which might make the compounds poor enzyme substrates.
Tarasiuk
J
J
Department of Pharmaceutical Technology and Biochemistry, Technical University of Gdańsk, Poland.
Stefańska
B
B
Borowski
E
E
D000818:Animals; D002241:Carbohydrates / Q000737:chemistry; D003630:Daunorubicin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D004579:Electron Transport; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D009243:NAD / Q000378:metabolism*; D009245:NADH Dehydrogenase / Q000378:metabolism; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D051381:Rats; D011919:Rats, Inbred Strains
D002241:Carbohydrates; D009243:NAD; D009245:NADH Dehydrogenase; C018979:5-iminodaunorubicin; D003630:Daunorubicin