Early transient ultrastructural and quantitative changes in nucleolar vacuoles of sympathetic neurons in isolated superior cervical ganglion of the rat.
Nucleolar changes and their time-course following axotomy and decentralization were investigated using an ultrastructural and stereological analysis of sympathetic neurons in superior cervical ganglion. By comparison with ganglia fixed immediately after removal, incubation for 2 h, 3 h and 5 h in NCTC 109 medium at 37 degrees C was found to induce early changes in the nucleolar volume (first a 33% increase, followed by a 43% decrease and a subsequent recovery to control values after 5 h). Moreover, dense material (also called 'microspherules') appeared in nucleolar vacuoles as early as 2 h after the beginning of incubation and was present after 3 h, but disappeared after a 5 h incubation. The dense material may have been either fibrillar component involved in rDNA transcription, or granular component involved in the storage and/or transport of preribosomal particles. Such early, transient and quantitative changes were observed very earlier than in previous studies on the axon reaction.
Robaglia
A
A
Laboratoire de Biologie Cellulaire et Tissulaire, Faculté de Médecine, Marseille, France.
Bottini
J
J
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D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure*; D005728:Ganglia, Sympathetic / Q000166:cytology / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D009474:Neurons / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014617:Vacuoles / Q000648:ultrastructure*