Isolation and identification of islet amyloid polypeptide in normal human pancreas.
To identify islet amyloid polypeptide (IAPP) present in normal human pancreas, we isolated the peptide from a soluble peptide fraction of amyloid deposit-free pancreata of two non-diabetic patients by using reverse-phase high performance liquid chromatography coupled with a radioimmunoassay specific for human IAPP. IAPP(1-37) and IAPP(17-37) were isolated and their complete amino acid sequences were determined up to the C-terminus. Identification of IAPP in normal human pancreas suggests the possible biological function of IAPP as a novel pancreatic hormone in humans.
Nakazato
M
M
Third Department of Internal Medicine, Miyazaki Medical College, Japan.
Asai
J
J
Miyazato
M
M
Matsukura
S
S
Kangawa
K
K
Matsuo
H
H
D000328:Adult; D000595:Amino Acid Sequence; D000682:Amyloid / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D058228:Islet Amyloid Polypeptide; D007515:Islets of Langerhans / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011863:Radioimmunoassay
D000682:Amyloid; D058228:Islet Amyloid Polypeptide