Comparison of cultured human mononuclear cells, Limulus amebocyte lysate and rabbits in the detection of pyrogens.
Isolated human mononuclear cells exposed to either lipopolysaccharide or Staphylococcus aureus secreted interleukin-1 like material. The secretion was concentration dependent. The minimal detectable level in the test solution of lipopolysaccharide and Staphylococcus aureus was 200 pg/ml and 10(5) cells/ml respectively. The sensitivity and specificity of the Limulus Amebocyte Lysate test, the rabbit pyrogen test and the monocyte test are compared. The monocyte test is proposed as an alternative in-vitro test to the rabbit pyrogen test.
Hansen
E W
EW
Royal Danish School of Pharmacy, Department of Biological Sciences, Copenhagen.
Christensen
J D
JD
D000818:Animals; D002478:Cells, Cultured; D004731:Endotoxins / Q000032:analysis; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008033:Limulus Test; D008070:Lipopolysaccharides / Q000494:pharmacology; D011749:Pyrogens / Q000032:analysis*; D011817:Rabbits; D013211:Staphylococcus aureus
D004731:Endotoxins; D007375:Interleukin-1; D008070:Lipopolysaccharides; D011749:Pyrogens