[Changes in the synaptosomal membrane in the modelling of the phototherapeutic action used in bilirubin encephalopathy].
Continuous irradiation of bilirubin containing (3.10(-5) M) particles of the synaptosomal membrane (pH 7.2, at 10 degrees C) with blue light for 2 hours led to decrease of the total specific activity of ATPase, specific activity of Na, K-ATPase, and (less significantly and at a slower rate) specific activity of acetilcholinesterase. Pre-irradiation argon barbotage of the aqueous suspension of the synaptosomal membrane particles or addition of tocopherol acetate to the suspension counteracted the irradiation induced decrease of the enzyme activity. A counteraction was also produced by pre-irradiation alkalization of the suspension to pH 7.8 at 10 degrees C or addition of serum albumin to the suspension; the character of the effect of this protein on the activity of the membrane enzymes in irradiation was determined by certain peculiarities of its chemical composition. The strongest counteraction to the irradiation induced decrease of enzyme activity occurred in addition of monomeric albumin, freed of organophilic ligands, when pH of the suspension was 7.8 (10 degrees C). The activity of Na, K-ATPase and acetylcholinesterase was reduced most markedly when monomeric ligand containing albumin was added to a suspension of membrane particles which was acidified to pH 6.8 (10 degrees C) before the beginning of irradiation.
Tkachenko
A V
AV
D000818:Animals; D001663:Bilirubin / Q000378:metabolism / Q000528:radiation effects; D001921:Brain / Q000201:enzymology / Q000528:radiation effects*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000201:enzymology / Q000528:radiation effects*; D007647:Kernicterus / Q000378:metabolism / Q000628:therapy*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism / Q000528:radiation effects; D010789:Phototherapy / Q000379:methods*; D051381:Rats; D012709:Serum Albumin / Q000378:metabolism / Q000528:radiation effects; D013574:Synaptosomes / Q000201:enzymology / Q000528:radiation effects*; D013997:Time Factors
D008565:Membrane Proteins; D012709:Serum Albumin; D001663:Bilirubin
Izmeneniia v sinaptosomal'noĭ membrane pri modelirovanii fototerapevticheskogo vozdeĭstviia, primeniaemogo pri bilirubinovoĭ éntsefalopatii.