title: The effect of storage time on isolation of Leptospira interrogans from bovine kidneys.
abstract: Kidney tissues from 20 cattle infected with Leptospira interrogans serovars hardjo, pomona, or grippotyphosa were cultured on the day of slaughter and 3, 6, and 8 days later to examine the effect of storage time on the recovery of leptospires by conventional culture methods. Leptospires were isolated from 85% of infected bovine kidney tissues cultured on day 1, and from 95%, 90%, and 90% of kidney tissues stored in transport medium at 4 C for 3, 6, and 8 days, respectively, prior to inoculation of culture media.
authors.affiliation: National Veterinary Services Laboratories, US Department of Agriculture, Ames, IA 50010.
authors.forename: D A
authors.initials: DA
authors.lastname: Miller
authors.forename: M A
authors.initials: MA
authors.lastname: Wilson
authors.forename: G W
authors.initials: GW
authors.lastname: Beran
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D007668:Kidney / Q000382:microbiology*; D007921:Leptospira interrogans / Q000302:isolation & purification*; D012034:Refrigeration; D013048:Specimen Handling / Q000662:veterinary*; D013997:Time Factors; D014895:Weil Disease / Q000382:microbiology / Q000662:veterinary*