title: Electrostatic assay of phospholipase A activity: an application of the second harmonic method of monitoring membrane boundary potentials.
abstract: To evaluate phospholipase A activity a new assay is suggested. This assay is based on the recording of boundary potential changes of the planar bilayer lipid membrane during enzymatic hydrolysis of lipids. To register these changes, a second harmonic method is used. Sensitivity of the assay is about 0.0002 units/ml regardless of the impurities that may be present in the samples. One analysis takes about 5 min.
authors.affiliation: Frumkin Institute of Electrochemistry U.S.S.R. Academy of Sciences, Moscow.
authors.forename: V M
authors.initials: VM
authors.lastname: Mirsky
authors.forename: V V
authors.initials: VV
authors.lastname: Cherny
authors.forename: V S
authors.initials: VS
authors.lastname: Sokolov
authors.forename: V S
authors.initials: VS
authors.lastname: Markin
mesh_terms: D004560:Electricity; D008051:Lipid Bilayers / Q000378:metabolism; D008564:Membrane Potentials / Q000502:physiology; D010741:Phospholipases A / Q000378:metabolism*
chemical_list: D008051:Lipid Bilayers; D010741:Phospholipases A