Isoform, kinetic and immunochemical characterization of rodent liver alpha-L-fucosidases.
alpha-L-Fucosidase from hamster and six inbred mouse strains contains two to three unique basic isoelectric forms (above pI 7.0) in addition to the usual acidic and neutral isoforms from pI 4-7. Rat liver alpha-L-fucosidase contains multiple isoforms between pI values of 4.0 and 7.3 whereas guinea pig liver alpha-L-fucosidase exhibits a single broad isoform at pI 5.3. 2. All the alpha-L-fucosidases have similar KM values (0.05-0.12 mM) for 4-methylumbelliferyl-alpha-L-fucopyranoside but pH-activity curves which are significantly different in optima and per cent of optimal activity in the acid region. 3. Double-antibody immunoprecipitation experiments indicate that rodent liver alpha-L-fucosidases crossreact to varying extents with polyclonal antibody against human liver alpha-L-fucosidase. 4. Hamster, guinea pig and mouse liver alpha-L-fucosidases exhibit significantly less binding than human and rat liver fucosidases to the agarose-epsilon-aminocaproylfucosamine affinity resin.
Johnson
S W
SW
Department of Chemistry, Lehigh University, Bethlehem, PA 18015.
Alhadeff
J A
JA
D000818:Animals; D002846:Chromatography, Affinity; D006224:Cricetinae; D006168:Guinea Pigs; D006863:Hydrogen-Ion Concentration; D007163:Immunosorbent Techniques; D007526:Isoelectric Point; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008647:Mesocricetus; D051379:Mice; D008806:Mice, Inbred AKR; D008810:Mice, Inbred C57BL; D013045:Species Specificity; D013379:Substrate Specificity; D005644:alpha-L-Fucosidase / Q000737:chemistry* / Q000378:metabolism
D005644:alpha-L-Fucosidase