Identification of 3,6,7,12-tetrahydroxy-5 beta-cholan-24-oic acids in human biologic fluids.
Unusual bile acids, 3 alpha, 6 alpha, 7 alpha, 12 alpha-, and 3 alpha, 6 beta, 7 beta, 12 alpha-tetrahyroxy-5 beta-cholan-24-oic acids, were identified in all amniotic fluid (four samples) and urine (six samples) from adult patients with cholestatic liver disease by gas-liquid chromatography/mass spectrometry. For the certain identification of these bile acids in the biologic samples, the chemical syntheses of 3 alpha, 6 beta, 7 alpha, 12 alpha- and 3 alpha, 6 beta, 7 beta, 12 alpha-tetrahydroxy-5 beta-cholan-24-oic acids were conducted.
Yoshii
M
M
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, Japan.
Kihira
K
K
Shoda
J
J
Osuga
T
T
Hoshita
T
T
D000653:Amniotic Fluid / Q000737:chemistry*; D002777:Cholestanols / Q000032:analysis* / Q000652:urine; D002779:Cholestasis / Q000652:urine; D006801:Humans; D015394:Molecular Structure
D002777:Cholestanols