A new, rapid, one-step method for the isolation of platelets from human blood.
We describe a new, rapid method for the isolation of platelets from human blood using a single centrifugation step through Nycodenz, an inert, nontoxic, nonionic medium. As judged by aggregation and nucleotide release studies, the platelets are recovered in high yield and in excellent functional condition.
Ford
T C
TC
Department of Cellular and Molecular Sciences, St. Georges Hospital Medical School, London, UK.
Graham
J
J
Rickwood
D
D
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism / Q000502:physiology*; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods; D004912:Erythrocytes / Q000378:metabolism / Q000502:physiology; D006801:Humans; D010974:Platelet Aggregation
D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate