Immunocytochemical localization of glucocorticoid receptor in human gingival fibroblasts and evidence for a colocalization of glucocorticoid receptor with cytoplasmic microtubules.
The cellular distribution of the glucocorticoid receptor (GR) in relation to various intracellular and plasma membrane structures in human fibroblasts was studied using indirect immunofluorescence techniques with monoclonal and polyclonal antibodies. During interphase, GR was located predominantly in the cytoplasm, showing a similar pattern as tubulin. In mitotic cells, GR and tubulin were localized in mitotic spindles and in telophase midbodies. Colchicine and vinblastine induced a similar redistribution of GR and tubulin to the cell periphery. This redistribution was reversible for colchicine but not for vinblastine. Vinblastine also induced paracrystals containing GR and tubulin. These results support the hypothesis that GR interacts in vivo with cytoplasmic microtubules.
Akner
G
G
Department of Medical Nutrition, Karolinska Institutet, Huddinge University Hospital, Sweden.
Sundqvist
K G
KG
Denis
M
M
Wikström
A C
AC
Gustafsson
J A
JA
D015153:Blotting, Western; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D005881:Gingiva / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008870:Microtubules / Q000378:metabolism*; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D014747:Vinblastine / Q000494:pharmacology
D011965:Receptors, Glucocorticoid; D014747:Vinblastine; D003078:Colchicine