[Effects of sodium tanshinone II-A sulfonate on slow action potentials of dissociated single ventricular cells of adult guinea pig heart].
The sodium channels of dissociated single ventricular cells of adult guinea pig heart were inactivated by partial depolarization in high K+ (25 mmol/L) Tyrode's solution and slow response action potential was elicited by intracellular stimulation. An obvious inhibition of the response was observed in the presence of 20 mumol/L sodium tanshinone II-A sulfonate (DS-201). In the concentration range from 1 mumol/L to 20 mumol/L, the inhibition effect of sodium tanshinone II-A sulfonate on the slow response action potentials enhanced by 0.28 mumol/L isoprenaline is concentration-dependent. Moreover, the inhibition effects of sodium tanshinone II-A sulfonate become stronger with the increase (in the range of 6.9 nmol/L to 0.55 mumol/L) of isoprenaline. The above-mentioned results suggest that sodium tanshinone II-A sulfonate may be a kind of effective calcium channel blocker. Under the effect of high concentration (50-100 mumol/L) of sodium tanshinone II-A sulfonate, the amplitude of fast response action potential of dissociated ventricular myocytes of adult guinea pig was decreased and the time to reach the peak was prolonged. All these results indicate that sodium channels were blocked to a certain extent by the high concentration of sodium tanshinone II-A sulfonate.
Liu
Q Y
QY
Shanghai Institute of Physiology, Chinese Academy of Sciences.
Tsai
T D
TD
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002317:Cardiovascular Agents / Q000494:pharmacology*; D005260:Female; D006168:Guinea Pigs; D008297:Male; D009206:Myocardium / Q000166:cytology*; D010616:Phenanthrenes / Q000494:pharmacology*; D016276:Ventricular Function
D002317:Cardiovascular Agents; D010616:Phenanthrenes; C024894:tanshinone II A sodium sulfonate