Metabolism and effect on cholesterol metabolism of 3 alpha-hydroxy-7-hydroxyimino-5 beta-cholanoic acid in hamsters.
The metabolic fate of a bile acid analog, 3 alpha-hydroxy-7-hydroxyimino-5 beta-cholanoic acid, was studied in hamsters. This compound was absorbed rapidly from the intestine and secreted into the bile as either taurine- or glycine-conjugates, at the rate similar to that of chenodeoxycholic acid. The ratio of glycine to taurine conjugates for this bile acid analog, 0.2, was much smaller than that for chenodeoxycholic acid, 2.0. After oral administration of a single dose of the labeled analog to intact hamsters, radioactivity was recovered in faces but not in urine. A major metabolite found in the feces was lithocholic acid (60%), whereas unchanged material was present only in a trace amount (2.4%). After the hamsters were fed chow supplemented with 0.075% of this bile acid analog for 21 d, analysis of the gallbladder bile acids revealed that the administered compound accounted for only 1.6% of total bile acids. The biliary bile acid composition was similar to that of chenodeoxycholic acid fed group. In the strain of hamster studied, feeding of the bile acid analog decreased cholesterol absorption significantly (19% decrease, p less than 0.05), and tended to reduce serum and liver cholesterol concentrations.
Miki
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S
Department of Surgery I, Kyushu University School of Medicine, Fukuoka, Japan.
Une
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Hoshita
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T
D000818:Animals; D001646:Bile / Q000378:metabolism; D001659:Biliary Tract / Q000378:metabolism; D001711:Biotransformation; D002635:Chenodeoxycholic Acid / Q000378:metabolism / Q000494:pharmacology / Q000652:urine; D002784:Cholesterol / Q000097:blood / Q000378:metabolism* / Q000493:pharmacokinetics; D002791:Cholesterol, Dietary / Q000378:metabolism; D002793:Cholic Acids / Q000378:metabolism / Q000494:pharmacology*; D002855:Chromatography, Thin Layer; D006224:Cricetinae; D007408:Intestinal Absorption / Q000187:drug effects; D008099:Liver / Q000378:metabolism; D008297:Male; D008647:Mesocricetus; D014316:Tritium
D002791:Cholesterol, Dietary; D002793:Cholic Acids; D002635:Chenodeoxycholic Acid; D014316:Tritium; C068239:3-hydroxy-7-(hydroxyimino)cholanic acid; D002784:Cholesterol