Non-invasive 31P NMR study on the development of brain membrane of gerbils and jimpy mice, a myelin-deficient mutant.
The broad background resonance observed in the in vivo 31P NMR spectra of adult murine heads was investigated in terms of phosphorus atoms in bone and membrane phospholipids. The broad background resonance was found to be weak in a juvenile and increase with advance of age. Fractionation of adult gerbil heads showed that the broad signal was derived from bone and membrane, of which myelin is the major component. The two origins of the broad background resonance exhibited considerably different line shapes in spectra, which enabled us to extract the membrane component from an intact murine head spectrum (sequential subtraction method). By the use of this method, the development of membrane in gerbil brain at various age grades could be estimated. The membrane component was shown to be suppressed in a mutant mouse, jimpy, which has a deficiency in myelin formation ability. Furthermore, the value of T1 of the membrane component was estimated to be 0.9 sec, which was in good agreement with previously reported values for excised brain.
Kanashiro
M
M
Department of Biochemistry, National Cardiovascular Center Research Institute, Osaka, Japan.
Naritomi
H
H
Sasaki
M
M
Tsuji
S
S
Miyake
Y
Y
D000818:Animals; D001842:Bone and Bones / Q000737:chemistry / Q000378:metabolism; D001921:Brain / Q000254:growth & development* / Q000378:metabolism; D001923:Brain Chemistry; D002457:Cell Extracts / Q000737:chemistry; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D005260:Female; D005849:Gerbillinae; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D051379:Mice; D008816:Mice, Jimpy; D008818:Mice, Neurologic Mutants; D009186:Myelin Sheath / Q000737:chemistry / Q000378:metabolism; D012535:Scalp / Q000737:chemistry; D012886:Skull / Q000737:chemistry
D002457:Cell Extracts