A low-cost automated device incorporating a hollow fiber filtration cartridge for large-scale production of ghosts from human erythrocytes.
Readily available elements were used to build an automatic apparatus dedicated to the preparation of erythrocyte ghosts. The apparatus is designed around a low-cost re-usable hollow-fiber filtration cartridge (marketed for therapeutic plasmapheresis). The apparatus is controlled by a simple programmer (based on a diode matrix and low cost timers and liquid level sensors): once the apparatus is loaded with whole red blood cells, washing of cells, as well as hemolysis and washing of ghosts, is performed by the machine in about 4.5 h without any operator intervention. Automatic filter cleaning takes a further 110 min.
el Ouggouti
S
S
INSERM U160, Hôpital Beaujon, Clichy, France.
Truskolaski
A
A
Bussel
A
A
Dhermy
D
D
Bertrand
O
O
D001331:Automation / Q000191:economics; D002458:Cell Fractionation / Q000295:instrumentation*; D004910:Erythrocyte Membrane; D005374:Filtration / Q000295:instrumentation; D006801:Humans; D010956:Plasmapheresis / Q000295:instrumentation*