Effect of indomethacin on materno-fetal amino acid transfer in the dual-perfused human placental cotyledon.
Indomethacin (1, 10 and 100 mumol L-1) was incrementally infused (8 min for each concentration) into the maternal and fetal circulations of dual-perfused human placental lobules, and measurements were made of materno-fetal amino acid transfer (using the non-metabolizable amino acid alpha-aminoisobutyric acid, or AIB) and fetal effluent perfusate prostaglandin (PG) E levels. No significant changes in AIB transfer compared with controls were observed at any of the doses of indomethacin infused. There was, however, a significant dose-dependent reduction compared with controls in PGE in the fetal circulation with the infusion of indomethacin.
Krishna
B R
BR
Department of Pharmacology, Monash University, Clayton, Vic. Australia.
King
R G
RG
Gu
W
W
Brennecke
S P
SP
D000596:Amino Acids / Q000378:metabolism*; D000621:Aminoisobutyric Acids / Q000378:metabolism*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology*; D008431:Maternal-Fetal Exchange; D010477:Perfusion; D010920:Placenta / Q000098:blood supply / Q000378:metabolism*; D011247:Pregnancy; D011458:Prostaglandins E / Q000378:metabolism
D000596:Amino Acids; D000621:Aminoisobutyric Acids; D011458:Prostaglandins E; C100049:2-aminoisobutyric acid; D007213:Indomethacin