title: Assay for the soluble interleukin-2 receptor by sandwich enzyme linked immunosorbent assay.
abstract: A system for the detection of soluble IL-2R by sandwich enzyme linked immunosorbent assay (ELISA) was established. This assay system has good reproducibility, and was found to be specific for soluble IL-2R by examination of the binding of anti-IgM antibody or anti-IL-2R monoclonal antibody (mAb) with IgM or soluble IL-2R and inhibition by IL-2 of the binding of anti-IL-2R mAb to soluble IL-2R. The IL-2R molecules in supernatant of phytohemagglutinin-stimulated peripheral blood mononuclear cells were of 44,000-61,000 molecular weight as estimated by size of exclusion high performance liquid chromatography. The results of determinations for soluble IL-2R with a Eurogenetics kit (sandwich enzyme linked immunosorbent assay) were significantly correlated with those by the our method.
authors.affiliation: Department of Clinical Pathology, Hiroshima University School of Medicine.
authors.forename: M
authors.initials: M
authors.lastname: Takaishi
authors.forename: M
authors.initials: M
authors.lastname: Akiyama
authors.forename: T
authors.initials: T
authors.lastname: Hayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Hirai
authors.forename: Y
authors.initials: Y
authors.lastname: Murakami
authors.forename: R
authors.initials: R
authors.lastname: Ueda
authors.forename: K
authors.initials: K
authors.lastname: Tatsugawa
authors.forename: M
authors.initials: M
authors.lastname: Yamakido
authors.forename: T
authors.initials: T
authors.lastname: Tsubokura
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis; D007376:Interleukin-2 / Q000494:pharmacology; D008970:Molecular Weight; D015375:Receptors, Interleukin-2 / Q000032:analysis* / Q000276:immunology; D012995:Solubility
chemical_list: D007075:Immunoglobulin M; D007376:Interleukin-2; D015375:Receptors, Interleukin-2