Design of 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde as a reagent for ultrasensitive determination of primary amines by capillary electrophoresis using laser fluorescence detection.
Amino acids and peptides, from both standard solutions and biological samples, were successfully reacted with 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde, at low concentration, to form highly fluorescent isoindole derivatives. The formed mixtures are effectively separated by high-performance capillary electrophoresis and their constituents detected by their laser-induced fluorescence signals. The minimum detectable quantities in the low attomole (10(-18) mol) range are encountered.
Liu
J P
JP
Department of Chemistry, Indiana University, Bloomington 47405.
Hsieh
Y Z
YZ
Wiesler
D
D
Novotny
M
M
D000588:Amines / Q000032:analysis*; D000596:Amino Acids / Q000032:analysis; D001565:Benzoates / Q000737:chemistry*; D004586:Electrophoresis; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007834:Lasers; D010455:Peptides / Q000032:analysis; D011804:Quinolines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D000588:Amines; D000596:Amino Acids; D001565:Benzoates; D007202:Indicators and Reagents; D010455:Peptides; D011804:Quinolines; C069617:3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde