Direct radioimmunoassay for diethylstilbestrol in serum.
A radioimmunoassay method for the measurement of diethylstilbestrol directly on 50 microliters of human serum was established using a tritium-labeled radioligand and a double antibody as a separation reagent. The assay was sensitive (less than 0.17 micrograms/L), reproducible (intra-assay and interassay coefficient of variation values, 8.14% and 8.25%, respectively), and accurate (recovery up to 95%). Factors influencing the assay are identified and discussed.
Economou
E V
EV
National Center for Scientific Research Demokritos, Athens, Greece.
Kakabakos
S E
SE
Evangelatos
G P
GP
Ithakissios
D S
DS
D001798:Blood Proteins / Q000494:pharmacology; D004054:Diethylstilbestrol / Q000097:blood* / Q000627:therapeutic use; D000431:Ethanol / Q000494:pharmacology; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000097:blood / Q000188:drug therapy; D011863:Radioimmunoassay / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D012709:Serum Albumin / Q000494:pharmacology
D001798:Blood Proteins; D012709:Serum Albumin; D000431:Ethanol; D004054:Diethylstilbestrol