title: A radioimmunoassay for the determination of insulins from several animal species, insulin derivatives and insulin precursors in both their native and denatured state.
abstract: Antibodies were raised against the carboxy terminus of the insulin A chain in sheep, goat and rabbit using as antigen the synthetic octapeptide YQLENYCN conjugated to bovine serum albumin (BSA). All of the antisera obtained cross-reacted with molecules containing this peptide sequence. Using these antibodies, we developed a radioimmunoassay that could detect the insulin A chain itself, both denatured and natural mammalian and avian insulins, as well as proinsulins and insulin fusion proteins from microorganisms.
authors.affiliation: Hoechst AG, F.R.G.
authors.forename: S
authors.initials: S
authors.lastname: Müllner
authors.forename: H
authors.initials: H
authors.lastname: Neubauer
authors.forename: W
authors.initials: W
authors.lastname: König
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D007328:Insulin / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D011384:Proinsulin / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods*; D013045:Species Specificity
chemical_list: D007328:Insulin; D010455:Peptides; D011384:Proinsulin