Identification of the rhamnose-sensitive adhesin of Capnocytophaga ochracea ATCC 33596.
The coaggregation of the Gram-negative microorganism Capnocytophaga ochracea ATCC 33596 with several Gram-positive bacteria, including streptococci, actinomyces and rothia, is mediated by rhamnose-sensitive adhesin(s). In the present study, MAbs against the C. ochracea adhesin(s) were prepared. These antibodies inhibited all the rhamnose-sensitive interactions, indicating that they recognize epitopes at or near the rhamnose binding site of the adhesin. The monoclonals served as probes in immunoblot analysis and recognized a polypeptide of Mr 155 K present in the wild-type organism but absent in a coaggregation defective mutant. It is concluded that the rhamnose-sensitive interactions of C. ochracea are mediated by a 155 K Mr polypeptide present on its outer surface.
Weiss
E I
EI
Maurice and Gabriela School of Dental Medicine, Tel Aviv University, Ramat Aviv, Israel.
Eli
I
I
Shenitzki
B
B
Smorodinsky
N
N
D000188:Actinobacillus / Q000502:physiology; D000911:Antibodies, Monoclonal; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000276:immunology; D002206:Capnocytophaga / Q000032:analysis* / Q000502:physiology; D015151:Immunoblotting; D012210:Rhamnose / Q000032:analysis*; D013298:Streptococcus sanguis / Q000502:physiology
D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D012210:Rhamnose