Mutagenicity of N-nitrosomorpholine biosynthesized from morpholine in the presence of nitrate and its inhibition by ascorbic acid.
N-nitrosomorpholine biosynthesis and inhibition in mice treated with nitrate, morpholine and ascorbic acid, was studied by means of the mutagenic activity in urine samples. The preincubation method was used and Salmonella typhimurium TA 1535 was the test strain. The results suggest that N-nitrosomorpholine biosynthesis was carried out when the animals were fed high doses of nitrate, however ascorbic acid was an inhibitor of this process.
Pérez
A
A
Institute of Nutrition and Food Hygiene, Havana, Cuba.
Fernández
S I
SI
Garcia-Roché
M O
MO
de las Cagigas
A
A
Castillo
A
A
Fonseca
G
G
Herrera
M
M
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009025:Morpholines / Q000378:metabolism*; D016296:Mutagenesis / Q000187:drug effects*; D009152:Mutagenicity Tests; D009566:Nitrates / Q000494:pharmacology*; D009602:Nitrosamines / Q000378:metabolism / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains
D009025:Morpholines; D009566:Nitrates; D009602:Nitrosamines; C002741:N-nitrosomorpholine; D001205:Ascorbic Acid