High-performance liquid chromatographic method for the determination of polycyclic hydrocarbon metabolite distributions.
Analytical separations and quantification of eight metabolites of benzo[a]pyrene, nine metabolites of 7-methylbenz[c]acridine and nine metabolites of dibenz[a,j]acridine have been carried out on two reversed-phase high-performance liquid chromatographic systems using 3- and 10-microns C8 packings. Quantitative results for the distributions of primary metabolites for the two systems closely agreed and showed that up to 70% savings in chromatographic time could be achieved with the 3 micron particle size columns.
Sugiyanto
Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, Indonesia.
Holder
G M
GM
Ryan
A J
AJ
D000166:Acridines / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001564:Benzo(a)pyrene / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D066298:In Vitro Techniques; D008748:Methylcholanthrene / Q000494:pharmacology; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism; D010316:Particle Size; D011083:Polycyclic Compounds / Q000737:chemistry / Q000378:metabolism*; D051381:Rats
D000166:Acridines; D011083:Polycyclic Compounds; C022289:dibenzacridine; C031277:7-methylbenz(c)acridine; D001564:Benzo(a)pyrene; D008748:Methylcholanthrene