Ornithine decarboxylase in Saccharomyces cerevisiae: chromosomal assignment and genetic mapping of the SPE1 gene.
The gene for ornithine decarboxylase in Saccharomyces cerevisiae, SPE1, has been assigned to chromosome XI by the technique of transverse alternating pulsed field electrophoresis and DNA-DNA hybridization. Genetic mapping by tetrad analysis shows that the SPE1 gene is located on the left arm of chromosome XI, 6 cM from the LAP1 gene and 43 cM from the TRP3 gene. The spe10 mutation previously isolated in this laboratory is mapped to the N-terminal region of the SPE1 gene, and therefore should be designated as a spe1 allele.
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Laboratory of Biochemical Pharmacology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
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Tabor
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D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D015342:DNA Probes; D004271:DNA, Fungal / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D005816:Genetic Complementation Test; D009154:Mutation; D009693:Nucleic Acid Hybridization; D009955:Ornithine Decarboxylase / Q000235:genetics*; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*
D015342:DNA Probes; D004271:DNA, Fungal; D009955:Ornithine Decarboxylase