A general-purpose pulsed field controller.
A pulsed field controller compatible with various types of electrophoresis tanks and power supplies is described. The system is based on a high-precision timing circuit, with an output isolated from the main high voltage supply, and four high voltage transistors. The switching time is programmed manually between 5-160 s. With a transverse alternating field electrophoresis system, intact yeast chromosomes could be completely separated after 24 h.
Abbal
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P
Institut des Produits de la Vigne, Institut National de la Recherche Agronomique, Montpellier, France.
Picou
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C
D015825:Chromosomes, Fungal / Q000648:ultrastructure; D004586:Electrophoresis / Q000295:instrumentation*; D015203:Reproducibility of Results