title: Fragment tagging.
abstract: A tactic known as fragment tagging, which has proven to be exceptionally useful in expediting DNA cloning and plasmid construction schemes, is described. The advantage of fragment tagging is that it facilitates the isolation of specific plasmid DNA molecules present in small amounts within mixed pools of DNA. Four examples that illustrate several variations of the fragment tagging concept are presented.
authors.affiliation: Department of Biochemistry, Purdue University, West Lafayette, IN 47907.
authors.forename: J H
authors.initials: JH
authors.lastname: Zeilstra-Ryalls
authors.forename: R L
authors.initials: RL
authors.lastname: Somerville
mesh_terms: D003001:Cloning, Molecular; D004247:DNA / Q000096:biosynthesis / Q000737:chemistry*; D011088:DNA Ligases; D004351:Drug Resistance / Q000235:genetics; D005819:Genetic Markers; D010957:Plasmids; D015183:Restriction Mapping
chemical_list: D005819:Genetic Markers; D004247:DNA; D011088:DNA Ligases