Characterization of contaminants in EMS-associated L-tryptophan samples by high-performance liquid chromatography.
To identify chemical contaminant(s) associated with eosinophilia-myalgia syndrome (EMS), case and control lots of tryptophan were analyzed by HPLC with both UV and FL detection. Numerous contaminant peaks appeared on the chromatograms and some of them were identified as 5-hydroxytryptophan, indol aldehyde, indol, etc; from the retention time of authentic compounds. Among these, three peaks were significantly associated with case lots. One corresponds to di-tryptophan aminal of aldehyde (peak E). Others are unknown contaminants, UV-5 (FL-7) and UV-28 (FL-36). The structural elucidation and toxicological implication of UV-5 (FL-7) are currently in progress.
Toyo'oka
T
T
National Institute of Hygienic Sciences, Tokyo, Japan.
Yamazaki
T
T
Tanimoto
T
T
Sato
K
K
Sato
M
M
Toyoda
M
M
Ishibashi
M
M
Yoshihira
K
K
Uchiyama
M
M
D002851:Chromatography, High Pressure Liquid; D004340:Drug Contamination; D004802:Eosinophilia / Q000139:chemically induced*; D006801:Humans; D009135:Muscular Diseases / Q000139:chemically induced*; D010146:Pain / Q000139:chemically induced; D013577:Syndrome; D014364:Tryptophan / Q000009:adverse effects / Q000032:analysis*
D014364:Tryptophan