Use of isolated adult myocytes to evaluate cardiotoxicity. II. Preparation and properties.
The preparation and properties of isolated adult cardiac myocytes are reviewed, with the goal being to evaluate their usefulness as a model system for measuring cardiotoxicity. Some important factors in cell isolation methodology which impact on the quality of the preparation are identified, along with criteria for assessing the quality of cells after isolation. By all criteria, myocytes isolated by good procedures appear to largely retain their original properties. Moreover, the distinctive behavior of adult myocytes under metabolic stress endows them with a particular usefulness as monitors of toxicity. Overall, we conclude that the art of adult heart cell isolation and culture is now sufficiently advanced for either freshly isolated cells in suspension or cells in culture to be a useful model system for toxicity studies.
Haworth
R A
RA
Department of Anesthesiology, University of Wisconsin, Madison 53792.
D000818:Animals; D002118:Calcium / Q000502:physiology; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D006321:Heart / Q000187:drug effects / Q000502:physiology; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism; D014116:Toxicology / Q000379:methods
D002118:Calcium