Dopamine synthesis and release in LLC-PK1 cells.
A porcine-derived renal epithelial cell line, LLC-PK1, was used to examine dopamine (DA) synthesis from L-dopa and DA release into the media. DA synthesis and release were elevated by increasing either L-dopa concentration or incubation time. DA synthesis was shown to be entirely due to conversion of L-dopa to DA by aromatic amino acid decarboxylase. DA release from LLC-PK1 cells was stimulated by NaCl and other Na+ or Cl- salts. DA release by LLC-PK1 cells was not dependent on extracellular Ca2+ or significantly stimulated by the depolarizing actions of KCl. LLC-PK1 cells which are devoid of any neural contributions to renal DA production can synthesize DA from L-dopa and release DA in response to stimulation by either Na+ or Cl-.
Dawson
R
R
Department of Pharmacodynamics, University of Florida, Gainesville 32610.
Phillips
M I
MI
D000818:Animals; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000378:metabolism; D002395:Catecholamines / Q000378:metabolism; D002460:Cell Line; D004298:Dopamine / Q000096:biosynthesis*; D007668:Kidney / Q000166:cytology; D007980:Levodopa / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D013552:Swine
D002395:Catecholamines; D007980:Levodopa; D011189:Potassium Chloride; D001142:Aromatic-L-Amino-Acid Decarboxylases; D011188:Potassium; D004298:Dopamine