title: Enzyme immunoassay of testosterone using nitrocellulose discs as the solid phase.
abstract: An enzyme immunoassay (EIA) for the measurement of serum testosterone has been developed using nitrocellulose paper discs as the solid support. The paper discs (6 mm diameter) coated with testosterone-specific antibody were incubated with testosterone and testosterone-peroxidase conjugate in glass tubes. The amount of testosterone present in samples could be estimated from the bound peroxidase activity. The assay was validated by comparison with a microtitre plate-based ELISA and a commercial radioimmunoassay (RIA) kit. The correlation coefficient between RIA and EIA was 0.84. No significant cross reactivity was observed with other steroids, except for dihydrotestosterone. The inter- and intra-assay coefficients of variation were 4.4 and 9.6% respectively. The preservation and transport of the coated paper discs are convenient and the overall cost of the method is less than that of other methods.
authors.affiliation: Indian Institute of Chemical Biology, Calcutta.
authors.forename: A K
authors.initials: AK
authors.lastname: Samanta
authors.forename: E
authors.initials: E
authors.lastname: Ali
mesh_terms: D003101:Collodion; D003429:Cross Reactions; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation* / Q000592:standards / Q000706:statistics & numerical data; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013739:Testosterone / Q000097:blood* / Q000592:standards
chemical_list: D013739:Testosterone; D003101:Collodion