[Determination of interacting segments of tRNA(Leu) from cow mammary glands with homologous aminoacyl-tRNA-synthetase by a chemical modification method].
The interaction of the cow mammary gland tRNA(IAGLeu), having a long variable loop, with the cognate aminoacyl-tRNA synthetase has been studied by the alkylation with ethylnitrosourea. It was shown that leucyl-tRNA synthetase protects from alkylation 3'-phosphates of the nucleotides 12-13 in D-loop, 23-24 in D-stem and 37-43 in the anticodon arm of tRNA(IAGLeu). All regions of interaction with the aminoacyl-tRNA synthetase are located in the same plane of tRNA whereas the long variable loop is in another plane.
Petrushenko
Z M
ZM
Tukalo
M A
MA
Gudzera
O I
OI
Rozhko
O T
OT
Matsuka
G Kh
GKh
D000478:Alkylation; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012356:RNA, Transfer, Leu / Q000235:genetics* / Q000378:metabolism
D012356:RNA, Transfer, Leu; D000604:Amino Acyl-tRNA Synthetases
Opredelenie uchastkov vzaimodeĭstviia tRNK(Leu) iz molochnoĭ zhelezy korov s gomologichnoĭ aminoatsil-tRNK-sintetazoĭ metodom khimicheskoĭ modifikatsii.