Continuous-flow chemiluminescence determination of isoniazid by oxidation with N-bromosuccinimide.
A rapid and precise continuous-flow method is described for the determination of isoniazid (0.050-20.0 micrograms ml(-1)) based on the chemiluminescence produced during its reaction with N-bromosuccinimide in alkaline medium. The emission intensity is greatly enhanced by the presence of ammonia. The procedure is automated and samples can be analysed at a rate of 112 solutions per hour with a relative error of about 2%. When applied to tablets, the method is relatively free from interferences from common excipients and co-existing compounds. The results obtained for the assay of commercial preparations compared well with those obtained by an official chemical method and demonstrated good accuracy and precision.
Halvatzis
S A
SA
Laboratory of Analytical Chemistry, University of Athens, Greece.
Timotheou-Potamia
M M
MM
Calokerinos
A C
AC
D000641:Ammonia; D001322:Autoanalysis / Q000592:standards / Q000706:statistics & numerical data; D001974:Bromosuccinimide; D007538:Isoniazid / Q000032:analysis*; D049449:Luminescence; D010084:Oxidation-Reduction; D011786:Quality Control; D013501:Surface-Active Agents
D013501:Surface-Active Agents; D000641:Ammonia; D001974:Bromosuccinimide; D007538:Isoniazid