Production of various marker substances in human ovarian cancer cell lines of epithelial origin.
Some characteristics of cell biology and the production of various tumor markers were examined using 8 human ovarian cancer cell lines of epithelial origin. Structural abnormalities of chromosomes 1, 3, and 6 were relatively common karyotypic changes among the cell lines. Cytoplasmic estradiol or progesterone receptor was not detected in any of the cell lines. A significant heterogeneity of the production of various tumor markers (ferritin, tissue peptide antigen, carcinoembryonic antigen, carbohydrate antigens 125, and 19-9) was noted among the cell lines grown in culture medium supplemented with serum. Three of the 8 cell lines were adapted to proliferate in completely synthetic serum-free culture medium. In addition to marker substances described above, small amounts of progesterone or human chorionic gonadotropin were produced in 2 of the 3 cell lines grown in serum-free culture medium. These results indicate that various marker substances including tumor markers are not produced consistently by human ovarian cancer cells of epithelial origin.
Sekiya
S
S
Department of Obstetrics and Gynecology, Chiba University School of Medicine, Japan.
Tanaka
N
N
Takamizawa
H
H
D000230:Adenocarcinoma / Q000473:pathology; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis*; D002272:Carcinoembryonic Antigen / Q000032:analysis; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D003536:Cystadenocarcinoma / Q000473:pathology; D004715:Endometriosis / Q000473:pathology; D004848:Epithelium / Q000473:pathology; D005260:Female; D005293:Ferritins / Q000032:analysis; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology*; D010455:Peptides / Q000032:analysis; D011960:Receptors, Estrogen / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis; D019396:Tissue Polypeptide Antigen; D014407:Tumor Cells, Cultured / Q000473:pathology*
D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D010455:Peptides; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D019396:Tissue Polypeptide Antigen; D005293:Ferritins