title: Modulatory effect of metal ions on the immune response of fish: in vivo and in vitro influence of MnCl2 on NK activity of carp pronephros cells.
abstract: The in vivo and in vitro influence of MnCl2 on carp pronephros cells was investigated. Increased cytotoxicity against both YAC-1 and P 815 target cells was observed following an intraperitoneal injection of 40, 80, or 120 micrograms MnCl2/g body wt administrated 24 hr prior to the in vitro 51Cr release assay. Similarly, in vitro treatment of carp pronephros cells, at a final concentration of 60 micrograms/culture, resulted in an increase of NK cell activity in both YAC-1 and P 815 target cell lines. However, a significant decrease in this activity was shown with lower doses of MnCl2 (40 and 20 micrograms/culture).
authors.affiliation: Laboratoire d'Immunophysiologie Générale et Comparée, UER des Sciences, Limoges, France.
authors.forename: Z
authors.initials: Z
authors.lastname: Ghanmi
authors.forename: M
authors.initials: M
authors.lastname: Rouabhia
authors.forename: M
authors.initials: M
authors.lastname: Alifuddin
authors.forename: D
authors.initials: D
authors.lastname: Troutaud
authors.forename: P
authors.initials: P
authors.lastname: Deschaux
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D002347:Carps; D002460:Cell Line; D002712:Chlorides; D002860:Chromium Radioisotopes; D007274:Injections, Intraperitoneal; D007668:Kidney / Q000187:drug effects* / Q000276:immunology; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology; D008345:Manganese / Q000276:immunology; D017895:Manganese Compounds; D020149:Manganese Poisoning; D009374:Neoplasms, Experimental / Q000276:immunology
chemical_list: D002712:Chlorides; D002860:Chromium Radioisotopes; D017895:Manganese Compounds; D008345:Manganese; C025340:manganese chloride