Vimentin expression in normal human keratinocytes grown in serum-free defined MCDB 153 medium.
We have shown that subcultured keratinocytes derived from normal human skin and grown on plastic in serum-free defined medium (MCDB 153, with 0.1 mM Ca2+ concentration) synthesize vimentin. A fibrillar vimentin expression was observed in the cell cytoplasm by immunohistochemistry with different monoclonal antibodies to vimentin in a high proportion of cultured cells. Two dimensional gel electrophoresis of cytoskeletal proteins from keratinocyte subcultures and immunoblotting reaction with different monoclonal antibodies to vimentin showed a specific reaction in a position corresponding to vimentin. No cross-reaction with keratin polypeptides was obtained. This expression of vimentin may be related to the adaptation of cells to in vitro growth conditions.
Richard
M H
MH
Laboratoire de Recherche Dermatologique et Immunologie, INSERM U. 209, CNRS URA 601, Hôpital E. Herriot, Lyon, France.
Viac
J
J
Reano
A
A
Gaucherand
M
M
Thivolet
J
J
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D005347:Fibroblasts / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D015603:Keratinocytes / Q000378:metabolism*; D051379:Mice; D012016:Reference Values; D014746:Vimentin / Q000096:biosynthesis*
D003470:Culture Media; D014746:Vimentin