Effects of calcium channel blockers and hydralazine on epinephrine-induced stimulation of glucose output from primary cultured rat hepatocytes.
Effects of calcium channel blockers from structurally different classes and hydralazine on epinephrine-induced stimulation of glucose output from primary cultured rat hepatocytes were examined in vitro. Diltiazem, verapamil, and nifedipine dose-dependently inhibited the epinephrine-induced stimulation of glucose output from the primary cultured rat hepatocytes. In contrast to these agents, nicardipine caused decreases in the basal and epinephrine-stimulated glucose output from rat hepatocytes in primary culture. Hydralazine treatment was found to reduce the response in a dose-dependent manner. These results indicate that the calcium channel blockers and hydralazine did not potentiate but reduce the epinephrine-induced stimulation of glucose output from primary cultures of rat hepatocytes in vitro.
Sunaga
K
K
Group of Biochemical Pharmacology, Faculty of Pharmaceutical Sciences, Josai University, Saitama, Japan.
Ogihara
M
M
D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002478:Cells, Cultured; D004837:Epinephrine / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D006830:Hydralazine / Q000494:pharmacology*; D007545:Isoproterenol / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D002121:Calcium Channel Blockers; D006830:Hydralazine; D005947:Glucose; D007545:Isoproterenol; D009638:Norepinephrine; D004837:Epinephrine