[Isoelectric focusing of pure human pancreatic juice. 1. Examination of patients with and without pancreatic diseases].
Thin layer urea isoelectric focusing is a powerful tool for a rapid and reproducible high resolution separation of cholecystokinin stimulated human pancreatic proteins. In pancreatic juice of patients without pancreatic disease, 11 main protein bands were separated and distinguished by Coomassie Blue-R 250 staining. A number of faint protein bands divided among the whole pH range demonstrate a wide microheterogeneity of exocrine pancreatic proteins. Secretory protein patterns of patients with chronic pancreatitis are shown to be different from those of patients without pancreatic disease. Significant alterations were found at the acidic protein band region of pI 4.4 to 4.7. In two cases of chronic pancreatitis the IEF pattern was very similar to that obtained by a short time enteropeptidase incubation of pure pancreatic juice from patients without pancreatic disease.
Appelt
G
G
Forschungsabteilung Gastroenterologie, Bereichs Medizin, Universität Leipzig.
Hänsler
M
M
Rogos
R
R
D002766:Cholecystokinin; D006801:Humans; D007202:Indicators and Reagents; D007525:Isoelectric Focusing / Q000379:methods; D008970:Molecular Weight; D010182:Pancreatic Diseases / Q000503:physiopathology*; D010189:Pancreatic Juice / Q000737:chemistry* / Q000187:drug effects; D011506:Proteins / Q000302:isolation & purification*; D012016:Reference Values
D007202:Indicators and Reagents; D011506:Proteins; D002766:Cholecystokinin
Isoelektrische Fokussierung von reinem menschlichen Pankreassaft. 1. Mitteilung: Untersuchungen des Pankreassaftes von Patienten mit und ohne Pankreaserkrankungen.