Decreased activity of liver coproporphyrinogen oxidase in hexachlorobenzene-induced porphyria.
A 2.5-time decrease of hepatic coproporphyrinogen oxidase activity was established in mice fed for 8 weeks with a diet containing 0.02% hexachlorobenzene. The enzyme activity was determined by a newly developed technique measuring directly the unconverted substrate, coproporphyrinogen III, with reverse phase HPLC (high-performance liquid chromatography) after its spontaneous oxidation to coproporphyrin.
Adjarov
D G
DG
Institute of Gastroenterology and Nutrition, Bulgarian Medical Academy, Sofia.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003304:Coproporphyrinogen Oxidase / Q000378:metabolism*; D003306:Coproporphyrins / Q000378:metabolism; D006581:Hexachlorobenzene / Q000009:adverse effects*; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000201:enzymology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010084:Oxidation-Reduction; D011164:Porphyrias / Q000139:chemically induced* / Q000201:enzymology; D013997:Time Factors
D003306:Coproporphyrins; D006581:Hexachlorobenzene; D003304:Coproporphyrinogen Oxidase