Immunocytochemical localization of chloramphenicol acetyltransferase as a single-cell marker of transfected and transgenic cells.
A technique is described for immunocytochemical localization of the bacterial gene product chloramphenicol acetyltransferase, which is a commonly used reporter gene in transfected and transgenic cells. The described procedure is capable of localizing the enzyme in individual cells, providing a means of determining the cell type(s) expressing a foreign construct in complex cultures or in tissue sections of transgenic mice.
Harlan
R E
RE
Dept. of Anatomy, Tulane University, School of Medicine, New Orleans, LA 70112.
Mondal
D
D
Coleman
R
R
Prakash
O
O
D000818:Animals; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000032:analysis* / Q000235:genetics; D015696:Gene Products, tat / Q000235:genetics; D005819:Genetic Markers; D016325:HIV Long Terminal Repeat / Q000235:genetics; D015497:HIV-1 / Q000235:genetics; D007150:Immunohistochemistry; D051379:Mice; D008822:Mice, Transgenic; D009457:Neuroglia; D014162:Transfection; D054322:tat Gene Products, Human Immunodeficiency Virus
D015696:Gene Products, tat; D005819:Genetic Markers; D054322:tat Gene Products, Human Immunodeficiency Virus; D015500:Chloramphenicol O-Acetyltransferase