[Recording of the free calcium content of the peripheral blood leukocytes in allergic diseases in children].
Flow cytometry was used to analyse the free calcium content in leukocytes of peripheral blood in children with allergic diseases, both before and after contacting with allergens and mediators of anaphylaxis. Computer analysis with a special mathematical program was used to evaluate the gravity-centre coordinate of the histograms obtained. More than a 10% shift to the right on the X-axis was diagnosed as an increased sensitivity of peripheral blood cells to the allergens or mediators of anaphylaxis. Good correlations were obtained between our data and different provocation tests, usually used in practical allergology.
Kirillov
M A
MA
Nisheva
E S
ES
Rozanov
Iu M
IuM
D000293:Adolescent; D000634:Aminoquinolines; D002118:Calcium / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D005434:Flow Cytometry / Q000379:methods; D005456:Fluorescent Dyes; D005512:Food Hypersensitivity / Q000097:blood; D006098:Granulocytes / Q000737:chemistry; D006801:Humans; D006967:Hypersensitivity / Q000097:blood*; D007223:Infant; D007962:Leukocytes / Q000737:chemistry*
D000634:Aminoquinolines; D005456:Fluorescent Dyes; C035739:Quin2; D002118:Calcium
Registratsiia soderzhaniia svobodnogo kal'tsiia v leĭkotsitakh perifericheskoĭ krovi pri allergicheskikh zabolevaniiakh u deteĭ.