A dual dominant selection, dual marker gene amplification model for environmental surveillance of recombinant viral vaccines.
A simple dual dominant selection marker gene model is proposed for recombinant DNA (rDNA) viral vaccines. Marker genes are also used as targets for in vitro DNA amplification (polymerase chain reaction). Environmentally stable and destabilized rDNA viruses are easily recovered and identified. Use of the model would facilitate evaluation of pressing environmental and safety issues surrounding release of rDNA herpesviruses and poxviruses into the biosphere.
Katz
J B
JB
Diagnostic Virology Laboratory, United States Department of Agriculture, Ames, IA 50010.
D004274:DNA, Recombinant / Q000276:immunology*; D004279:DNA, Viral; D004784:Environmental Monitoring; D005819:Genetic Markers; D006564:Herpesviridae / Q000235:genetics / Q000276:immunology*; D008957:Models, Genetic; D016133:Polymerase Chain Reaction; D011212:Poxviridae / Q000235:genetics / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000235:genetics; D014765:Viral Vaccines / Q000235:genetics*
D004274:DNA, Recombinant; D004279:DNA, Viral; D005819:Genetic Markers; D014614:Vaccines, Synthetic; D014765:Viral Vaccines