title: Effect of stimulation of leukocyte chemotaxis by fMLP on white blood cell behaviour in the microcirculation of rat brain.
abstract: The role of white blood cells in acute cerebral disorders such as ischemia or stroke is still unclear. Therefore, in the present study we investigated the effects of the leukotaxin n-formyl-methionyl-leucyl-phenylalanine (fMLP) on white blood cell endothelial-cell interactions in the rat brain surface microcirculation. An improved closed cranial window technique was applied. Superfusion of fMLP in rising concentrations (10(-8) - 10(-5) M) was seen to induce rolling and adherence of leukocytes to teh endothelium of small venules. Rolling was more effectively stimulated than firm attachment. fMLP-induced vasodilation was more pronounced in arterioles than in venules. In this study it has been shown that the hydrophilic fMLP is effectively stimulating neutrophil chemotaxis across the blood-brain barrier. Further, the closed cranial window preparation is useful to analyze quantitatively properties of activated leukocytes, which may be pertinent in injury to the blood-brain barrier and induction of microcirculatory disturbances.
authors.affiliation: Institute for Surgical Research, Klinikum Grosshadern, Ludwig-Maximilians-Universität München, Federal Republic of Germany.
authors.forename: S
authors.initials: S
authors.lastname: Corvin
authors.forename: L
authors.initials: L
authors.lastname: Schürer
authors.forename: C
authors.initials: C
authors.lastname: Abels
authors.forename: O
authors.initials: O
authors.lastname: Kempski
authors.forename: A
authors.initials: A
authors.lastname: Baethmann
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002560:Cerebrovascular Circulation; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology; D007962:Leukocytes / Q000502:physiology*; D008297:Male; D008833:Microcirculation / Q000187:drug effects; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D014664:Vasodilation / Q000187:drug effects
chemical_list: D009240:N-Formylmethionine Leucyl-Phenylalanine