Preparative scale separation of neutral lipids and phospholipids by centrifugally accelerated thin-layer chromatography.
Mixtures of lipids and phospholipids were separated by centrifugally accelerated thin-layer chromatography on a preparative scale (300-500 mg lipid mixture per run). The isolated lipids and phospholipids were identified by 1H and 13C NMR spectroscopy and their fatty acid composition was determined by GLC and GLC-MS of their methyl esters.
Bergheim
S
S
Institute of Pharmacy, University of Oslo, Norway.
Malterud
K E
KE
Anthonsen
T
T
D002498:Centrifugation; D002855:Chromatography, Thin Layer / Q000379:methods*; D005227:Fatty Acids / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D008055:Lipids / Q000737:chemistry / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D010743:Phospholipids / Q000737:chemistry / Q000302:isolation & purification*
D005227:Fatty Acids; D008055:Lipids; D010743:Phospholipids