Pseudoepidermis, constructed in vitro, for use in toxicological and pharmacological studies.
The purpose of this study was to establish the validity of the stratified, cornified keratinocyte culture as a model for investigating cutaneous toxicities. This pseudoepidermis, grown on a nylon membrane at the air-liquid interface, responded to topical application of a known vesicant similarly to the response of the tissue in vivo. Alterations in the morphology of the in vitro model also resembled pathological changes seen in in vivo models after exposure to this agent. The effects of the skin irritants benzoate and salicylate on protein and DNA synthesis in the culture were also similar to those observed in vivo.
Scavarelli-Karantsavelos
R M
RM
Department of Environmental and Industrial Health, School of Public Health, University of Michigan, Ann Arbor.
Zaman Saroya
S
S
Vaughan
F L
FL
Bernstein
I A
IA
D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography; D001565:Benzoates / Q000494:pharmacology; D019817:Benzoic Acid; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects; D004121:Dimethyl Sulfoxide; D007509:Irritants / Q000494:pharmacology; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D008954:Models, Biological; D009151:Mustard Gas / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism; D051381:Rats; D012459:Salicylates / Q000494:pharmacology; D020156:Salicylic Acid; D012867:Skin / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
D001565:Benzoates; D007509:Irritants; D011506:Proteins; D012459:Salicylates; D019817:Benzoic Acid; D004247:DNA; D007930:Leucine; D020156:Salicylic Acid; D009151:Mustard Gas; D013936:Thymidine; D004121:Dimethyl Sulfoxide