Fluorescent location of human colonic tumour cells by means of an enzyme-inhibitor complex.
We studied the enzymic status of the tumour cell surface protease, guanidinobenzoatase (GB) in frozen sections of a human colonic tumour grown in nude mice and also in human colons. Active enzyme was demonstrated by the binding of a synthetic fluorescent probe for the active centre of guanidinobenzoatase (GB). It was observed that tissue derived inhibitors of GB blocked the binding of this fluorescent probe and that enzyme inhibitor complex formation could be controlled by lowering the pH of the medium with lactic acid. The presence of an inhibitor of GB in the mouse tumour extract was taken advantage of by making two fluorescent derivatives of this inhibitor; both of which located GB on colonic tumour cells in frozen sections of human colon.
Steven
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FS
Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Manchester, U.K.
Maier
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Talbot
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D000585:Aminacrine; D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000032:analysis* / Q000037:antagonists & inhibitors; D003110:Colonic Neoplasms / Q000201:enzymology / Q000473:pathology*; D010450:Endopeptidases; D005456:Fluorescent Dyes; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence; D009368:Neoplasm Transplantation; D011480:Protease Inhibitors / Q000032:analysis*; D014183:Transplantation, Heterologous
D005456:Fluorescent Dyes; D011480:Protease Inhibitors; D000585:Aminacrine; D002265:Carboxylic Ester Hydrolases; C037288:guanidinobenzoate esterase; D010450:Endopeptidases