Automated liquid chromatographic determination of ranitidine in microliter samples of rat plasma.
An improved high-performance liquid chromatographic method with UV detection at 313 nm has been developed for quantitation of ranitidine in 100 microliter of rat plasma over the range 25 to 1000 ng/ml. To each sample were added the internal standard (metiamide) and 2 M NaOH. After dichloromethane extraction, the nitrogen-dried extracts were reconstituted in the mobile phase of 0.01 M phosphate buffer-triethylamine-methanol-water (530:5:390:75 v/v). Chromatography on mu Bondapak C18 with quantitation by peak height ratios showed an analyte recovery of 97%; a limit of detection of 10 ng/ml; a precision of 1-10% and an accuracy of 1-5%. About 90 samples can be processed in 24 h.
Segelman
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Department of Chemical Biology and Pharmacognosy, College of Pharmacy, Rutgers-The State University, Piscataway, NJ 08854.
Adusumalli
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Segelman
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D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D011899:Ranitidine / Q000097:blood*; D051381:Rats; D014466:Ultraviolet Rays
D011899:Ranitidine