Simultaneous determination of ampicillin and sulbactam by liquid chromatography: post-column reaction with sodium hydroxide and sodium hypochlorite using an active hollow-fibre membrane reactor.
A high-performance liquid chromatographic method has been developed for the simultaneous determination of ampicillin (ABPC) and sulbactam (SBT) in serum and urine. The method involves separation of ABPC and SBT from the background components of serum and urine on a C18 column, post-column reaction with sodium hydroxide and sodium hypochlorite using an active hollow-fibre membrane reactor, and detection at 270 nm. At ABPC and SBT concentrations of 10 and 5 micrograms/ml in urine and serum samples, the precisions (relative standard deviations) were 0.9-2.5% (n = 8). The detection limits were 20 and 5 ng for ABPC and SBT, respectively, at a signal-to-noise ratio of 3.
Haginaka
J
J
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Hyogo, Japan.
Nishimura
Y
Y
D000667:Ampicillin / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D012972:Sodium Hydroxide; D012973:Sodium Hypochlorite; D013407:Sulbactam / Q000032:analysis* / Q000097:blood / Q000652:urine
D012972:Sodium Hydroxide; D000667:Ampicillin; D012973:Sodium Hypochlorite; D013407:Sulbactam