A protein kinase C isozyme is translocated to cytoskeletal elements on activation.
Protein kinase C (PKC)1 isozymes comprise a family of related cytosolic kinases that translocate to the cell particulate fraction on stimulation. The activated enzyme is thought to be on the plasma membrane. However, phosphorylation of protein substrates occurs throughout the cell and is inconsistent with plasma membrane localization. Using an isozyme-specific monoclonal antibody we found that, on activation, this PKC isozyme translocates to myofibrils in cardiac myocytes and to microfilaments in fibroblasts. Translocation of this activated PKC isozyme to cytoskeletal elements may explain some of the effects of PKC on cell contractility and morphology. In addition, differences in the translocation site of individual isozymes--and, therefore, phosphorylation of different substrates localized at these sites--may explain the diverse biological effects of PKC.
Mochly-Rosen
D
D
Department of Neurology, University of California, San Francisco.
Henrich
C J
CJ
Cheever
L
L
Khaner
H
H
Simpson
P C
PC
D008841:Actin Cytoskeleton / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal; D001693:Biological Transport, Active; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism*; D004789:Enzyme Activation; D005455:Fluorescent Antibody Technique; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D009206:Myocardium / Q000201:enzymology* / Q000378:metabolism; D009210:Myofibrils / Q000378:metabolism; D010766:Phosphorylation; D011493:Protein Kinase C / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D011493:Protein Kinase C