title: A comparative study of two methods for the isolation of human leucocytes for DNA extraction.
abstract: The 'Dextran' and the 'Buffy-coat' methods for isolation of human leucocytes for DNA extraction were compared on the basis of DNA yield from the same amounts (10 ml) of blood. Human leucocytes from a total of 11 samples were isolated using both methods for each sample after which DNA was extracted. Extracted DNA samples were treated with ribonucleases and proteinase K after which the yields were quantitated by measuring absorbance at 260 nm. The 'Buffy-coat' method yielded a mean concentration of DNA of 476.7 micrograms/ml (range: 212 to 700 micrograms/ml) while the 'Dextran' method yielded 188.4 micrograms/ml (range: 64 to 340 micrograms/ml). The difference was confirmed by subjecting the extracted DNA samples to agarose gel electrophoresis.
authors.affiliation: Division of Haematology, Faculty of Medicine, National University of Malaysia, Kuala Lumpur.
authors.forename: L H
authors.initials: LH
authors.lastname: Lim
authors.forename: S H
authors.initials: SH
authors.lastname: Ton
authors.forename: S K
authors.initials: SK
authors.lastname: Cheong
mesh_terms: D001773:Blood Cells / Q000378:metabolism; D002469:Cell Separation / Q000379:methods*; D004247:DNA / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*
chemical_list: D004247:DNA