title: Molecular cloning of human satellite DNA sequences and their use in detecting DNA polymorphism.
abstract: A approximately 400 bp HaeIII human genomic satellite DNA band was cloned into pUC18 to construct a partial library. A fragment of bacteriophage M13 containing a sequence homologous to the human minisatellite core was cloned in pUC18 and was used as a probe to isolate a approximately 350 bp human satellite clone (pTRF5.6) from the partial library. Other clones from this library showed a wide variation in terms of size and hybridization to the pTRF5.6 clone. Human DNA from different individuals was digested with restriction enzymes, Southern transferred and probed with TRF5.6. Individual-specific complex pattern of DNA bands was produced. TRF5.6, therefore, could be useful as a probe for detecting genetic polymorphism.
authors.affiliation: National Institute of Immunology, Shaheed Jeet Singh Marg, New Delhi.
authors.forename: N Z
authors.initials: NZ
authors.lastname: Ehtesham
authors.forename: G P
authors.initials: GP
authors.lastname: Talwar
authors.forename: A
authors.initials: A
authors.lastname: Ali
authors.forename: S E
authors.initials: SE
authors.lastname: Hasnain
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004276:DNA, Satellite / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic
chemical_list: D004276:DNA, Satellite