Effects of succinylated concanavalin A on infectivity and syncytial formation of human immunodeficiency virus.
The effects of various lectins on the infectivity of human immunodeficiency virus (HIV) type 1 was investigated. Among the 25 lectins investigated, 2 types of concanavalin A (Con A) and 3 types of phytohemagglutinin were found to inhibit HIV infection. Succinylated Con A (S-Con A) efficiently blocked HIV-induced formation of syncytia in a coculture of MOLT-4 cells and blocked cell-free infection by HIV of MT-4 cells. The HIV-binding study revealed that S-Con A only partially inhibited viral binding to cells, although the control Leu-3a monoclonal antibody strongly inhibited it. When S-Con A was added to cultures after the initiation of viral adsorption, the number of HIV antigen-positive cells that developed depended on the time interval before addition of the compound. S-Con A inhibited HIV infection even after viral binding to cells at 0 degrees C and further incubation at 37 degrees C for 1 day. These data suggest that S-Con A inhibited mainly the fusion process rather than viral binding to cells in exerting its anti-HIV activity.
Matsui
T
T
Department of Virology and Parasitology, Yamaguchi University School of Medicine, Japan.
Kobayashi
S
S
Yoshida
O
O
Ishii
S
S
Abe
Y
Y
Yamamoto
N
N
D002459:Cell Fusion / Q000187:drug effects; D002460:Cell Line; D003208:Concanavalin A / Q000031:analogs & derivatives* / Q000494:pharmacology; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D006678:HIV / Q000187:drug effects* / Q000472:pathogenicity / Q000502:physiology; D006801:Humans; D037102:Lectins / Q000494:pharmacology; D011991:Receptors, Virus / Q000187:drug effects
D037102:Lectins; D011991:Receptors, Virus; D003208:Concanavalin A; C019558:succinylconcanavalin A