title: The influence of metal ions and some inhibitors on the activity of the proteinase isolated from the hatching liquid of Coregonus peled.
abstract: A specific proteinase (chorionase) was isolated from the hatching liquid, obtained by electric stimulation of the hatching glands in Coregonus peled embryos. The enzymatic preparation was electrophoretically homogeneous, and showed proteolytic activity towards the egg membranes. Ions of Na+ at concentration from 10(-5) to 10(-4) M activated the chorionase of C. peled, whereas ions of K+, Na+, Ca2+ and Mg2+ at concentrations above 10(-4) M inhibited the enzyme. Addition of EDTA to the incubation mixture significantly reduced the enzyme activity. The chorionase was not inhibited by the natural soybean trypsin inhibitor.
authors.affiliation: Department of Animal Biochemistry, Academy of Agriculture and Technology, Olsztyn-Kortowo, Poland.
authors.forename: Z
authors.initials: Z
authors.lastname: Luberda
authors.forename: J
authors.initials: J
authors.lastname: Strzezek
authors.forename: M
authors.initials: M
authors.lastname: Luczyński
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002823:Chorion; D004558:Electric Stimulation; D004625:Embryo, Nonmammalian; D010450:Endopeptidases / Q000378:metabolism*; D005399:Fishes; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008666:Metalloendopeptidases / Q000302:isolation & purification / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology*; D012964:Sodium / Q000494:pharmacology
chemical_list: D011480:Protease Inhibitors; D012964:Sodium; D010450:Endopeptidases; D008666:Metalloendopeptidases; C024344:envelysin; D008274:Magnesium; D011188:Potassium; D002118:Calcium