Current methods for YAC clone characterization.
Yeast artificial chromosome (YAC) cloning has allowed isolation of much longer DNA fragments than was previously possible. While this technology makes feasible the isolation of large regions of complex genomes in overlapping cloned segments, it has also required altered methods for manipulation of cloned DNAs. In particular, the isolation of insert sequences from YAC clones has been especially difficult. Methods for characterization of YAC inserts are described and compared.
Nelson
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DL
Institute for Molecular Genetics, Baylor College of Medicine, Houston, TX 77030.
D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D015723:Gene Library; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D015183:Restriction Mapping