An enzyme-linked immunoassay for human salivary albumin.
An ELISA method has been developed for the determination of albumin in human parotid saliva. The range of sensitivity is 2-80 micrograms/l and it is suitable for use with volumes of 5 microliters or less of freshly collected parotid fluid, without prior sample preparation. Calibration data were fitted by microcomputer to an exponential function. The albumin concentration of parotid saliva was 1.9 +/- 1.53 mg/l, but levels fell on sample storage. Isoelectric focusing, followed by immunoblotting with antiserum to human serum albumin, showed identical double bands of pI 4.9-5.0 in albumin from both serum and saliva, thereby confirming that the antigens from the two sources are identical.
Sweeney
D
D
Oral Biochemistry Unit (Oral Biology Group), University of Glasgow Dental Hospital and School, Scotland.
Beeley
J A
JA
D000293:Adolescent; D000328:Adult; D000418:Albumins / Q000032:analysis*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007525:Isoelectric Focusing; D008875:Middle Aged; D010306:Parotid Gland; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D012709:Serum Albumin / Q000032:analysis
D000418:Albumins; D007074:Immunoglobulin G; D012471:Salivary Proteins and Peptides; D012709:Serum Albumin