Binding of 2-amino-4-chloro-m-benzenedisulfonamide as a metabolite of hydrochlorothiazide to erythrocytes.
2-Amino-4-chloro-m-benzenedisulfonamide (ACBS) is a metabolite of hydrochlorothiazide. We reported that the ACBS concentration in erythrocytes was higher than in plasma in a patient. Therefore the binding of ACBS to rabbit erythrocyte was studied. The Scatchard plot showed the nonlinear plot and the horizontal asymptote. Curvature in this plot indicated the existence of 2 classes of binding. One class was at a specific site, probably at carbonic anhydrase. Chromatographic data seemed to support the possibility.
Yamazaki
M
M
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Itoh
S
S
Saji
J
J
Tanabe
K
K
Nakahama
H
H
Orita
Y
Y
D000818:Animals; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D006852:Hydrochlorothiazide / Q000097:blood*; D066298:In Vitro Techniques; D011817:Rabbits; D013424:Sulfanilamides / Q000097:blood* / Q000493:pharmacokinetics
D013424:Sulfanilamides; D006852:Hydrochlorothiazide; C054586:4-amino-6-chloro-1,3-benzenedisulfonamide