Alternative translation initiation site usage results in two structurally distinct forms of Pit-1.
Pit-1 is a pituitary-specific transcription factor that plays a critical role in the normal development of the anterior pituitary gland. Previous analyses have shown that this protein exists in the rat pituitary gland and in rat pituitary-derived cell lines as two forms of relative molecular mass 33 and 31 kDa. This aspect of Pit-1 expression has been conserved throughout the evolution from rodents to humans. Here, we determine the origin of these structurally distinct forms of Pit-1 protein and find that these arise as a consequence of the alternative usage of translation initiation sites present in Pit-1 mRNA.
Voss
J W
JW
Howard Hughes Medical Institute, University of California, San Diego, La Jolla 92093-0648.
Yao
T P
TP
Rosenfeld
M G
MG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D003062:Codon / Q000235:genetics; D004268:DNA-Binding Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D007106:Immune Sera; D008715:Methionine / Q000378:metabolism; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D010903:Pituitary Gland, Anterior / Q000502:physiology*; D014176:Protein Biosynthesis; D051381:Rats; D050817:Transcription Factor Pit-1; D014157:Transcription Factors / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection
D003062:Codon; D004268:DNA-Binding Proteins; D007106:Immune Sera; D010455:Peptides; C494186:Pou1f1 protein, rat; D050817:Transcription Factor Pit-1; D014157:Transcription Factors; D008715:Methionine