Transcellular permeation of nitrophenols through newborn rat skin epidermal cells in monolayer culture.
The permeation of nitrophenols through epidermal cells from newborn rat skin cultured on type IV collagen-coated Millipore filters was studied under various conditions. The order of permeation through the cultured skin cells was found to be p- greater than m- greater than o-nitrophenol at both 10 and 37 degrees C. This order was the same as that of their affinities to isolated skin cells. The permeation of nitrophenols was not inhibited by the inhibitors of energy transduction 2-deoxyglucose and NaN3. These results suggest that the permeation of nitrophenols across a cultured cell layer occurs by simple diffusion. The order of permeation of nitrophenols across newborn abdominal epidermis was exactly the opposite of that of their permeation across a cultured cell layer.
Ohkura
K
K
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Iwamoto
K
K
Terada
H
H
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D002478:Cells, Cultured; D008297:Male; D009596:Nitrophenols / Q000493:pharmacokinetics*; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D012869:Skin Absorption
D009596:Nitrophenols