Determination of antitubercular drugs in human plasma by HPLC through direct injection.
A simple and efficient method is described for the direct determination of Isoniazide (INH) and Pyrazinamide (PZAm) in human plasma, based on reversed-phase HPLC. A number of other antitubercular drugs such as p-Aminosalycylic acid (PAS), Streptomycin (STM) and Ethambutol (ETH) and the major metabolites of the two investigated drugs, namely N-Monoacetylisoniazide (AcINH) and Pyrazinoic acid (PZAc), were shown not to interfere with the assay method. A potential application of the method to routine clinical monitoring of antitubercular drugs is discussed.
Altomare
C
C
Dipartimento Farmaco-Chimico, Università di Bari, Italy.
Carotti
A
A
Cellamare
S
S
Leogrande
G
G
De Vito
D
D
D000995:Antitubercular Agents / Q000097:blood*; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D006801:Humans
D000995:Antitubercular Agents