title: Evolutionary aspects of superoxide dismutase: the copper/zinc enzyme.
abstract: Copper/zinc superoxide dismutase is typically an enzyme of eukaryotes. The presence of the enzyme in the ponyfish symbiont Photobacterium leiognathi and some free living bacteria does not have an immediate explanation. Amino acid sequence alignment of 19 Cu/Zn superoxide dismutases shows 21 invariant residues in key positions related to maintenance of the beta-barrel fold, the active site structure including the electrostatic channel loop, and dimer contacts. Nineteen other residues are invariant in 18 of the 19 sequences. Thirteen of these nearly invariant residues show substitutions in Photobacterium Cu/Zn superoxide dismutase. Copper/zinc superoxide dismutase from the trematode Schistosoma mansoni shows an N-terminal sub-domain with a hydrophobic leader peptide, as in human extracellular superoxide dismutase which is a Cu/Zn enzyme. The latter also has a C-terminal sub-domain with preponderance of hydrophilic and positively charged residues. The amino acid sequence of this superoxide dismutase between the N-terminal and C-terminal regions shares many features of cytosolic Cu/Zn superoxide dismutase, including 20 of the 21 invariant residues found in 19 Cu/Zn enzymes, suggesting a similar type of beta-barrel fold and active site structure for the extracellular enzyme.
authors.affiliation: Department of Physiology and Biochemistry, University of Malta, Msida.
authors.forename: W H
authors.initials: WH
authors.lastname: Bannister
authors.forename: J V
authors.initials: JV
authors.lastname: Bannister
authors.forename: D
authors.initials: D
authors.lastname: Barra
authors.forename: J
authors.initials: J
authors.lastname: Bond
authors.forename: F
authors.initials: F
authors.lastname: Bossa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D005075:Biological Evolution; D003300:Copper; D005110:Extracellular Space / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000235:genetics*; D015032:Zinc
chemical_list: D001426:Bacterial Proteins; D005656:Fungal Proteins; D010940:Plant Proteins; D003300:Copper; D013482:Superoxide Dismutase; D015032:Zinc