title: HLA-DR/Dw matching by PCR fingerprinting: the origin of PCR fingerprints and further applications.
abstract: Polymerase chain reaction (PCR) fingerprinting, a new method for the rapid matching of HLA-Dr/Dw allotypes, involves the visual comparison of polymorphic HLA-DRB gene second exon PCR products, resolved in non-denaturing polyacrylamide gels (Bidwell & Hui, 1990). We show here that the satellite DNA bands within PCR fingerprints originate by heteroduplex formation between heterologous DNAs co-amplified by a common PCR primer set. We also present two further applications of the technique which permit discrimination between unrelated HLA-DR/Dw allotypes with similar PCR fingerprints.
authors.affiliation: Molecular Genetics Laboratory, U.K. Transplant Service, Bristol.
authors.forename: N A
authors.initials: NA
authors.lastname: Wood
authors.forename: T M
authors.initials: TM
authors.lastname: Clay
authors.forename: J L
authors.initials: JL
authors.lastname: Bidwell
mesh_terms: D016172:DNA Fingerprinting / Q000379:methods*; D006684:HLA-DR Antigens / Q000145:classification / Q000235:genetics*; D006579:Heterozygote; D006650:Histocompatibility Testing / Q000379:methods; D006720:Homozygote; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D006684:HLA-DR Antigens