title: [Hepatitis B virus DNA. Current diagnosis in children with chronic hepatitis B].
abstract: After cloning the hepatitis B virus genome molecular hybridization techniques have been established for detection of HBV-DNA in serum and liver tissue. HBV-DNA is presently the most sensitive marker of viral replication and infectivity, which was previously related to the presence of HBeAg in serum and HBcAg in liver cells. HBV-DNA is usually analysed by dot blot-, Southern blot- and in situ hybridization techniques. The study of HBV-DNA has become a valuable part of the routine diagnostic in chronic hepatitis B, providing a more reliable estimation of viral replication and contagiousness and better parameters for severity and prognosis of the chronic infection.
authors.affiliation: Kinderklinik, Johannes-Gutenberg-Universität Mainz.
authors.forename: S
authors.initials: S
authors.lastname: Wirth
authors.forename: K M
authors.initials: KM
authors.lastname: Keller
authors.forename: W
authors.initials: W
authors.lastname: Baumann
authors.forename: B
authors.initials: B
authors.lastname: Zabel
mesh_terms: D015139:Blotting, Southern; D002648:Child; D015342:DNA Probes; D005814:Genes, Viral / Q000235:genetics; D006509:Hepatitis B / Q000175:diagnosis*; D006515:Hepatitis B virus / Q000235:genetics*; D006521:Hepatitis, Chronic / Q000175:diagnosis*; D006801:Humans; D015151:Immunoblotting
chemical_list: D015342:DNA Probes
vernacular_title: Hepatitis-B-Virus-DNS. Aktuelle Diagnostik bei Kindern mit chronischer Hepatitis B.