title: Immunologic measurement of polycyclic aromatic hydrocarbon-albumin adducts in foundry workers and roofers.
abstract: Roofers and iron foundry workers with high exposure to polycyclic aromatic hydrocarbons (PAH) were monitored for levels of covalent PAH serum albumin adducts, quantitated in enzymatically digested samples by a sensitive competitive enzyme-linked immunosorbent assay. Albumin adducts were higher in the foundry workers (5.22 fmol/micrograms, 0.314 mmol/mol) than in the reference group (4.07 fmol/micrograms, 0.245 mmol/mol), but only of borderline significance probably due to the small sample size. In a subset of foundry workers, a significant difference in adduct levels was observed for samples collected immediately after vacation and after six weeks of workplace exposure. The roofers also showed higher levels of adducts (5.19 fmol/micrograms, 0.312 mmol/mol) than their reference group (3.28 fmol/micrograms, 0.197 mmol/mol). These results demonstrate the feasibility of PAH protein adduct measurement as a marker of human exposure to this class of chemicals.
authors.affiliation: College of Pharmacy, Sung Kyun Kwan University, Su-Won, South Korea.
authors.forename: B M
authors.initials: BM
authors.lastname: Lee
authors.forename: B Y
authors.initials: BY
authors.lastname: Yin
authors.forename: R
authors.initials: R
authors.lastname: Herbert
authors.forename: K
authors.initials: K
authors.lastname: Hemminki
authors.forename: F P
authors.initials: FP
authors.lastname: Perera
authors.forename: R M
authors.initials: RM
authors.lastname: Santella
mesh_terms: D000911:Antibodies, Monoclonal; D001564:Benzo(a)pyrene / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008669:Metallurgy; D016273:Occupational Exposure; D011083:Polycyclic Compounds / Q000097:blood* / Q000276:immunology / Q000378:metabolism; D012709:Serum Albumin / Q000032:analysis* / Q000276:immunology / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D011083:Polycyclic Compounds; D012709:Serum Albumin; D001564:Benzo(a)pyrene