Cyclothiazomycin, a novel polythiazole-containing peptide with renin inhibitory activity. Taxonomy, fermentation, isolation and physico-chemical characterization.
Cyclothiazomycin is a novel renin inhibitor produced by Streptomyces sp. NR0516. It was isolated from fermentation broth by extraction with butyl alcohol, QAE-Toyopearl column chromatography and preparative HPLC. Cyclothiazomycin, which was determined to be a unique polythiazole-containing bicyclic peptide, exhibited inhibitory activity against human plasma renin with IC50 being 1.7 microM.
Aoki
M
M
Nippon Roche Research Center, Kanagawa, Japan.
Ohtsuka
T
T
Yamada
M
M
Ohba
Y
Y
Yoshizaki
H
H
Yasuno
H
H
Sano
T
T
Watanabe
J
J
Yokose
K
K
Seto
H
H
D000803:Angiotensin I / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011863:Radioimmunoassay; D012988:Soil Microbiology; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013302:Streptomyces / Q000145:classification / Q000378:metabolism*; D013844:Thiazoles / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000092502:Renin Inhibitors
D000803:Angiotensin I; D010456:Peptides, Cyclic; D013844:Thiazoles; D000092502:Renin Inhibitors; C069913:cyclothiazomycin