The monoclonal antibody RIB5/1--a useful completion of the MRC OX-3 and OX-6 haplotype specificity.
The haplotype binding pattern of a new mAb RIB5/1 against RT1 class II antigens on different tissues of inbred rat strains was investigated using the indirect POD-technique and a cytofluorometric analysis. RIB5/1 binds to a polymorphic determinant of the RT1 class II glycoprotein. Unlike MRC OX-3 (and OX-6), RIB5/1 reacts in optimal dilution with the RT1 haplotypes dvl, a, av, g, 1 but not with u. Therefore, the mAb pair MRC OX-3 (OX-6) and RIB5/1 is applicable for specific immunohistological purposes in chimaeric models with these RT1 haplotypes.
Lehmann
M
M
Institute of Biochemistry, University of Rostock.
Kuttler
B
B
Köhler
H
H
Hahn
H J
HJ
Volk
H D
HD
Brock
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D005434:Flow Cytometry; D006239:Haplotypes; D006649:Histocompatibility Antigens / Q000235:genetics / Q000276:immunology*; D007124:Immunoenzyme Techniques; D008214:Lymphocytes / Q000276:immunology; D008221:Lymphoid Tissue / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D006649:Histocompatibility Antigens; C038353:histocompatibility antigens RT, rat