Divergency of structure and function of vertebrate xanthine:NAD+ oxidoreductase.
Selection pressure for increasing metabolic flux through a define metabolic pathway affects the enzyme levels, enzyme structure and their kinetic properties. These aspects exemplified by xanthine oxidoreductase from vertebrates of various type of nitrogen excretion are discussed. Two trends in evolutionary kinetic changes of oxypurine hydroxylating activity could be distinguished. Changes in the subunit structure and kinetic properties suggest that the domain catalysing oxypurine hydroxylation and the one cooperating with NAD+ evolved through separate pathways.
Jezewska
M
M
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw.
D000818:Animals; D007700:Kinetics; D015394:Molecular Structure; D013329:Structure-Activity Relationship; D014714:Vertebrates; D014968:Xanthine Dehydrogenase / Q000737:chemistry / Q000378:metabolism*
D014968:Xanthine Dehydrogenase