Structure, tissue-specific expression, and transforming activity of the mouse met protooncogene.
A 6.7-kilobase met complementary DNA (cDNA) was isolated from a pcD cDNA library prepared from C3H mouse fibroblast cell line polyadenylated RNA. Sequence analysis of 6.7-kilobase met cDNA insert revealed that it contained the entire open reading frame and shared an overall homology of 88.1% with the human met gene. Using the mouse met cDNA as probe, high levels of met expression were observed in the kidney, brain, lung, skin, and embryonic tissue as well as in several factor responsive mouse myeloid cell lines. Under SV40 promoter control, the mouse met protooncogene cDNA in the pCD vector was able to transform NIH 3T3 cells. These transformed cells possess multiple copies of mouse met cDNA and exhibit properties of malignant cells, including growth in soft agar and induction of tumors in nude mice. Tumor explant cell lines analyzed by Western blot also reveal the presence of high levels of Mr 170,000 and 140,000 met protein product(s).
Iyer
A
A
Rush Presbyterian St. Luke's Medical Center, Medical Oncology Department, Chicago, Illinois 60612.
Kmiecik
T E
TE
Park
M
M
Daar
I
I
Blair
D
D
Dunn
K J
KJ
Sutrave
P
P
Ihle
J N
JN
Bodescot
M
M
Vande Woude
G F
GF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D004247:DNA / Q000235:genetics; D004790:Enzyme Induction; D005347:Fibroblasts / Q000187:drug effects; D051379:Mice; D008809:Mice, Inbred C3H / Q000235:genetics; D008819:Mice, Nude; D008969:Molecular Sequence Data; D009368:Neoplasm Transplantation; D009928:Organ Specificity; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D019859:Proto-Oncogene Proteins c-met; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D011518:Proto-Oncogene Proteins; D011993:Recombinant Fusion Proteins; D004247:DNA; D011505:Protein-Tyrosine Kinases; D019859:Proto-Oncogene Proteins c-met