Expression of calbindin D-28K-like immunoreactivity in human SK-N-SH and SH-SY-5Y neuroblastoma cells.
Calbindin D-28K is a calcium-binding protein first isolated from avian intestines, and more recently shown to be present in a number of other vertebrate species and in other tissues, including CNS neurones. Using immunocytochemical techniques and Western blotting, we have shown that a calbindin D-28K-like protein is expressed in two related human neuroblastoma cell lines, SK-N-SH and SH-SY-5Y. To our knowledge these are the first immortal neuronal cell lines shown to express calbindin D-28K.
Morton
A J
AJ
Institute of Animal Physiology & Genetics Research, Babraham, Cambridge, U.K.
Emson
P C
PC
D015153:Blotting, Western; D064026:Calbindins; D006801:Humans; D007124:Immunoenzyme Techniques; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009447:Neuroblastoma / Q000737:chemistry; D009474:Neurons / Q000737:chemistry*; D010641:Phenotype; D064030:S100 Calcium Binding Protein G / Q000032:analysis*; D014407:Tumor Cells, Cultured
D064026:Calbindins; D009419:Nerve Tissue Proteins; D064030:S100 Calcium Binding Protein G