title: Distribution of glycoconjugates in the optic vesicle and optic cup.
abstract: Lectin histochemical methods and immunohistochemical techniques have been utilized to investigate and partially characterize glycoconjugates in the developing eye. Peanut-lectin-binding sites associated with radial glial cells were found in the diencephalon. In the optic primordia, binding sites associated with radial glia were masked by terminal sialic acid, and only reacted with peanut lectin when pretreated with sialidase. This finding indicates that glycoconjugates associated with diencephalic radial glia contain terminal galactose-beta-(1----3)N-acetyl galactosamine, but glycoconjugates associated with radial glia in the optic primordia contain sialic acid----galactose-beta(1----3)N-acetyl galactosamine. The selective distribution of galactose, N-acetyl galactosamine and fucose associated with radial glial cells has also been demonstrated. We postulate that these distributions mediate the shaping of the developing eye.
authors.affiliation: Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599.
authors.forename: A J
authors.initials: AJ
authors.lastname: Alles
authors.forename: A R
authors.initials: AR
authors.lastname: Fazel
authors.forename: S S
authors.initials: SS
authors.lastname: Spicer
authors.forename: R M
authors.initials: RM
authors.lastname: Dom
mesh_terms: D000818:Animals; D001665:Binding Sites; D005123:Eye / Q000737:chemistry / Q000196:embryology*; D006001:Glycoconjugates / Q000096:biosynthesis*; D007124:Immunoenzyme Techniques; D007381:Intermediate Filament Proteins / Q000096:biosynthesis*; D037102:Lectins; D009024:Morphogenesis / Q000502:physiology; D009457:Neuroglia / Q000378:metabolism*; D019887:Peanut Agglutinin; D051381:Rats; D011919:Rats, Inbred Strains; D014746:Vimentin / Q000096:biosynthesis
chemical_list: D006001:Glycoconjugates; D007381:Intermediate Filament Proteins; D037102:Lectins; D019887:Peanut Agglutinin; D014746:Vimentin