Use of an Escherichia coli beta-glucuronidase gene as a reporter gene for investigation of Bacteroides promoters.
We have constructed transcriptional fusion vectors for use in Bacteroides spp., a genus of gram-negative obligate anaerobes found in high numbers in the human colon. The reporter group in these vectors is a promoterless beta-glucuronidase gene from Escherichia coli (uidA). Two of the vectors (pMJF-2 and pMJF-3) replicate in Bacteroides spp. The third, pCQW-1, does not replicate in Bacteroides spp. and can be used to introduce E. coli beta-glucuroindase fusions into the Bacteroides chromosome.
Feldhaus
M J
MJ
Department of Microbiology, University of Illinois, Urbana 61801.
Hwa
V
V
Cheng
Q
Q
Salyers
A A
AA
D001439:Bacteroides / Q000235:genetics*; D004261:DNA Replication; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D005822:Genetic Vectors; D005966:Glucuronidase / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping
D005966:Glucuronidase