title: Cloning of the nucleic acid-binding domain of the rat HnRNP C-type protein.
abstract: A cDNA encoding the nucleic acid-binding domain of the hnRNP C-type protein has been cloned by DNA-affinity screening of pituitary-derived expression libraries. An analysis revealed sequence identity with the human C-type cDNA and demonstrated the presence of a peptide sequence contained within the single-stranded DNA-binding protein, UP2, which was absent from the human cDNA. Structural analysis of the protein encoded by the rat cDNA demonstrated a net charge of +15 with 14.56% and 6.33% lysines and arginines, respectively, and an amino acid sequence that is consistent with an extensive helix-loop-helix-turn-helix structure.
authors.affiliation: Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284-78284.
authors.forename: Z D
authors.initials: ZD
authors.lastname: Sharp
authors.forename: K P
authors.initials: KP
authors.lastname: Smith
authors.forename: Z D
authors.initials: ZD
authors.lastname: Cao
authors.forename: S
authors.initials: S
authors.lastname: Helsel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000235:genetics; D015723:Gene Library; D034482:Heterogeneous-Nuclear Ribonucleoprotein Group C; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D006801:Humans; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000378:metabolism; D011487:Protein Conformation; D051381:Rats; D012261:Ribonucleoproteins / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D034482:Heterogeneous-Nuclear Ribonucleoprotein Group C; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D012261:Ribonucleoproteins; D004247:DNA