[Collagen as a carrier for heart cell cultures].
Extracellular collagen from rat tail (types 1 and 3) is a suitable matrix for isolated neonatal heart cell cultivation. The fine structure and the physiological response to isoproterenol for instance is similar for neonatal cardiomyocytes cultured in the medium Sm 20-1 with or without collagen matrix. For electron microscopical routine preparation it is easier to use matrix-grown cardiomyocytes because additional preparation steps (centrifugation) are unnecessary.
Schulze
W
W
Zentralinstitut für Herz-Kreislauf-Forschung, Akademie der Wissenschaften, DDR, Berlin-Buch.
Wallukat
G
G
Altankow
A
A
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D003094:Collagen; D005109:Extracellular Matrix; D006321:Heart / Q000187:drug effects / Q000502:physiology; D006352:Heart Ventricles; D007545:Isoproterenol / Q000494:pharmacology; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009206:Myocardium / Q000166:cytology* / Q000648:ultrastructure; D051381:Rats
D003094:Collagen; D007545:Isoproterenol
Kollagen als Carrier für Herzzellkulturen.