A new method for labelling of monoclonal antibodies and their fragments with technetium-99m.
A new method of labelling of antibodies with technetium-99m is described. The antibody or fragment is derivatized by iminothiolane. Ligand exchange from a labile technetium complex such as TcO(GLU)2- results in greater than 90% of the technetium labelling the antibody. This method appears to be equally applicable to antibodies and fragments.
Joiris
E
E
R/D Imaging, Medgenix Group, Fleurus, Belgium.
Bastin
B
B
Thornback
J R
JR
D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005337:Fibrin / Q000276:immunology; D006801:Humans; D007096:Imidoesters / Q000737:chemistry; D007128:Immunoglobulin Fragments / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry; D007553:Isotope Labeling; D013667:Technetium / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D007096:Imidoesters; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; C005905:methyl 4-mercaptobutyrimidate; D013667:Technetium; D005337:Fibrin