title: Comparison of two stocks of mice in spermatogonial response to different conditions of radiation exposure.
abstract: In a previous report (Generoso et al., 1985) it was shown that the two hybrid stocks of mice, (C3H/R1 x 101/R1)F1 and (SEC/R1 x C57BL/6)F1, differed in their responses to induction of chromosomal aberrations following exposure of the stem-cell spermatogonia to 500 R x 4 (4-week intervals) acute X-rays. The levels of response in the two stocks were paralleled by the effects on the length of the sterile period, which presumably results from stem-cell killing and repopulation. The present study was conducted in order to determine whether the differences between the two stocks in these parameters hold true also for other conditions of radiation exposure. Thus, comparative experiments were conducted using the following acute exposure regimens: 500 R single dose, 500 R + 500 R (24-h interval), 100 R + 900 R (24-h interval), and 500 R x 4 (8-week intervals). The endpoints measured were chromosome rearrangements in diakinesis/metaphase-I meiocytes, embryonic lethality in conceptuses, length of sterile period and testis weight. Trend analysis indicated that higher frequencies of chromosome rearrangements and embryonic lethality were recovered from (C3H/R1 x 101/R1)F1 than from (SEC/R1 x C57BL/6)F1 males, that there were no significant differences between stocks in testis weight reductions, and that there was no consistency in the direction of the significant differences that occurred in the length of the sterile period. A definitive conclusion regarding the possible association between induction of chromosomal aberrations and induction of cell killing awaits direct histological analysis of the stem-cell population.
authors.affiliation: Biology Division, Oak Ridge National Laboratory, TN 37731-8077.
authors.forename: W M
authors.initials: WM
authors.lastname: Generoso
authors.forename: K T
authors.initials: KT
authors.lastname: Cain
authors.forename: C V
authors.initials: CV
authors.lastname: Cornett
authors.forename: E L
authors.initials: EL
authors.lastname: Frome
mesh_terms: D000818:Animals; D002869:Chromosome Aberrations; D004307:Dose-Response Relationship, Radiation; D004622:Embryo, Mammalian / Q000528:radiation effects; D007248:Infertility, Male; D008297:Male; D051379:Mice; D013093:Spermatogonia / Q000528:radiation effects*; D013234:Stem Cells / Q000528:radiation effects; D013737:Testis / Q000528:radiation effects*; D014178:Translocation, Genetic