[3H]-13-cis-retinoic acid covalently binds to thioredoxin reductase in human keratinocytes.
13-cis-Retinoic acid is a stereospecific suicide inhibitor of thioredoxin reductase. [3H]-labeled 13-cis-retinoic acid has been used to covalently label thioredoxin reductase in human keratinocytes. The acid-soluble cytosol fraction of human keratinocytes contained three radioactive proteins labeled by the addition of high-specific-activity [3H]-13-cis-retinoic acid to cell cultures. One of these proteins was identified as cytosolic keratinocyte thioredoxin reductase by fast-protein liquid chromatography and SDS-gel radioautography. The inhibition of thioredoxin reductase by 13-cis-retinoic acid may explain the known cytostatic and teratogenic properties attributed to this retinoid.
Schallreuter
K U
KU
Department of Dermatology, University of Hamburg, FRG.
Grebe
T
T
Pittelkow
M R
MR
Wood
J M
JM
D002478:Cells, Cultured; D002853:Chromatography, Liquid; D006801:Humans; D015603:Keratinocytes / Q000201:enzymology / Q000378:metabolism*; D011485:Protein Binding; D013880:Thioredoxin-Disulfide Reductase / Q000378:metabolism*; D014212:Tretinoin / Q000378:metabolism*; D014316:Tritium
D014316:Tritium; D014212:Tretinoin; D013880:Thioredoxin-Disulfide Reductase