Characterization of a human gene conferring sensitivity to infection by gibbon ape leukemia virus.
Gibbon ape leukemia virus (GALV) enters cells following interaction with a specific receptor protein. We have isolated human complementary DNAs (cDNAs) encoding a protein which, when expressed in normally uninfectable mouse NIH3T3 cells, confers on these cells specific sensitivity to infection by GALV. This was done by transfection into mouse cells of human DNA and selection of putative receptor gene transfectants using infection with a retrovirus carrying a drug resistance gene. Transfected genomic sequences were then cloned through their association with repetitive DNA, and these were used to isolate cDNA clones. The predicted 679-amino acid sequence encoded in these cDNAs is characteristic of an integral membrane protein in that multiple potential transmembrane domains are present. Searches of DNA and protein data banks failed to reveal homologies to other known sequences. It thus appears that the sequence isolated is novel and represents the human receptor for GALV. As expected from the wide host range of the virus, closely related homologues of the gene were found in several other vertebrate species tested.
O'Hara
B
B
Molecular Biology Research Section, Lederle Laboratories, American Cyanamid Company, Pearl River, New York 10965.
Johann
S V
SV
Klinger
H P
HP
Blair
D G
DG
Rubinson
H
H
Dunn
K J
KJ
Sass
P
P
Vitek
S M
SM
Robins
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005347:Fibroblasts / Q000473:pathology; D005796:Genes; D020022:Genetic Predisposition to Disease; D006801:Humans; D008322:Mammals / Q000235:genetics; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011991:Receptors, Virus / Q000235:genetics / Q000502:physiology; D011994:Recombinant Proteins / Q000502:physiology; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014162:Transfection
D011991:Receptors, Virus; D011994:Recombinant Proteins; C067981:leukemia virus receptor, gibbon ape; D004247:DNA