Assay of hydroxyfarrerol in biological fluids.
A high-performance liquid chromatographic method for the determination of hydroxyfarrerol (IdB 1031) in biological samples was developed. IdB 1031 was first extracted by liquid-solid partition and the extracts were evaporated and analysed on a reversed-phase column under isocratic conditions, using either an electrochemical or a UV detector. The detection limit was ca. 5 ng/ml. Preliminary pharmacokinetic data showed that rats treated orally with 500 mg/kg had an average peak plasma concentration (Cmax) of 497 ng/ml after 2 h.
Marzo
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A
Drug Metabolism and Pharmacokinetic Department, Sigma-Tau SpA, Rome, Italy.
Arrigoni Martelli
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E
Bruno
G
G
Martinelli
E M
EM
Pifferi
G
G
D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000097:blood*; D051381:Rats
D005419:Flavonoids; C068573:IdB 1031