title: The use of paramagnetic beads for the detection of major histocompatibility complex class I and class II antigens.
abstract: Specific immunoadsorbents were prepared using paramagnetic particles (Dynabeads), and their ability to immunoprecipitate major histocompatibility complex (MHC) Class I and Class II antigens compared with conventional protein A Sepharose immunoadsorbents. Lysates of lymphoblastoid cells provided the antigen source which were visualized by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Dynabeads were found to be as effective as protein A Sepharose immunoadsorbents at immunoprecipitating MHC Class I and Class II antigens, but had a much lower nonspecific binding capacity resulting in fewer interference bands and lower backgrounds.
authors.affiliation: Department of Microbiology and Immunology, Duke University Medical Center, Durham, NC 27710.
authors.forename: A J
authors.initials: AJ
authors.lastname: Worlock
authors.forename: A
authors.initials: A
authors.lastname: Sidgwick
authors.forename: T
authors.initials: T
authors.lastname: Horsburgh
authors.forename: P R
authors.initials: PR
authors.lastname: Bell
mesh_terms: D002478:Cells, Cultured; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D007163:Immunosorbent Techniques; D007164:Immunosorbents; D008214:Lymphocytes / Q000276:immunology; D008280:Magnetics; D008863:Microspheres; D008970:Molecular Weight
chemical_list: D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D007164:Immunosorbents