Platelet-derived growth factor is detected in human blastocyst culture medium but not in human follicular fluid--a preliminary report.
The content of PDGF in human blastocyst culture medium (n = 8), serum (n = 12), and FF (n = 17) from natural IVF cycles was determined by an RIA specific for PDGF B-chain. The blastocysts were cultured under serum-free conditions throughout development. The findings show that PDGF B-chain is released into the culture medium of human blastocysts and that serum is positive, whereas FF is negative for PDGF.
Svalander
P C
PC
Sophiahemmet Hospital, Stockholm, Sweden.
Holmes
P V
PV
Olovsson
M
M
Wikland
M
M
Gemzell-Danielsson
K
K
Bygdeman
M
M
D001755:Blastocyst / Q000737:chemistry*; D002478:Cells, Cultured; D003470:Culture Media; D004624:Embryo Transfer; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000737:chemistry*; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000032:analysis*; D011247:Pregnancy; D011863:Radioimmunoassay
D003470:Culture Media; D010982:Platelet-Derived Growth Factor