Neonatal lethality and lymphopenia in mice with a homozygous disruption of the c-abl proto-oncogene.
The c-abl proto-oncogene, which encodes a cytoplasmic protein-tyrosine kinase, is expressed throughout murine gestation and ubiquitously in adult mouse tissues. However, its levels are highest in thymus, spleen, and testes. To examine the in vivo role of c-abl, the gene was disrupted in embryonic stem cells, and the resulting genetically modified cells were used to establish a mouse strain carrying the mutation. Most mice homozygous for the c-abl mutation became runted and died 1 to 2 weeks after birth. In addition, many showed thymic and splenic atrophy and a T and B cell lymphopenia.
Tybulewicz
V L
VL
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142.
Crawford
C E
CE
Jackson
P K
PK
Bronson
R T
RT
Mulligan
R C
RC
D000818:Animals; D000831:Animals, Newborn; D015139:Blotting, Southern; D003001:Cloning, Molecular; D005804:Genes, Lethal; D005818:Genetic Engineering; D006410:Hematopoiesis; D006579:Heterozygote; D006720:Homozygote; D008231:Lymphopenia / Q000235:genetics; D051379:Mice; D008817:Mice, Mutant Strains; D016315:Proto-Oncogene Proteins c-abl / Q000502:physiology*; D011519:Proto-Oncogenes; D011995:Recombination, Genetic; D013154:Spleen / Q000002:abnormalities; D013950:Thymus Gland / Q000002:abnormalities
D016315:Proto-Oncogene Proteins c-abl