Isolation and characterization of lipopolysaccharide from Centipeda periodontii ATCC 35019.
Lipopolysaccharide (LPS) was extracted from cells of Centipeda periodontii ATCC 35019 by a hot phenol-water method, purified by nuclease treatment and by repeated ultracentrifugations. The LPS was composed of 21.7% hexose, 9.1% hexosamine, 10.3% fatty acid, 0.5% protein, 2.5% phosphorus, 4.3% 2-keto-3-deoxyoctonate(KDO) and 2.3% heptose. The major fatty acids of the LPS were 3OH C13:0 and C13:0. C. periodontii LPS was able to gelatinize Limulus amebocyte and to produce the local Schwartzman reaction in rabbits. LPS from C. periodontii exhibited strong immunobiological activities, mitogenicity on splenocytes of C3H/HeN mice, and stimulation of interleukin 1 (IL-1) production in peritoneal macrophages of C3H/HeN mice.
Kokeguchi
S
S
Okayama University Dental School, Japan.
Tsutsui
O
O
Kato
K
K
Matsumura
T
T
D004731:Endotoxins; D005227:Fatty Acids; D006089:Gram-Negative Anaerobic Bacteria; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis; D008033:Limulus Test; D008070:Lipopolysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D008934:Mitogens; D010518:Periodontitis / Q000382:microbiology; D010636:Phenols; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000302:isolation & purification
D004731:Endotoxins; D005227:Fatty Acids; D007375:Interleukin-1; D008070:Lipopolysaccharides; D008934:Mitogens; D010636:Phenols; D011135:Polysaccharides, Bacterial