title: Cyclothiazomycin, a novel polythiazole-containing peptide with renin inhibitory activity. Taxonomy, fermentation, isolation and physico-chemical characterization.
abstract: Cyclothiazomycin is a novel renin inhibitor produced by Streptomyces sp. NR0516. It was isolated from fermentation broth by extraction with butyl alcohol, QAE-Toyopearl column chromatography and preparative HPLC. Cyclothiazomycin, which was determined to be a unique polythiazole-containing bicyclic peptide, exhibited inhibitory activity against human plasma renin with IC50 being 1.7 microM.
authors.affiliation: Nippon Roche Research Center, Kanagawa, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Aoki
authors.forename: T
authors.initials: T
authors.lastname: Ohtsuka
authors.forename: M
authors.initials: M
authors.lastname: Yamada
authors.forename: Y
authors.initials: Y
authors.lastname: Ohba
authors.forename: H
authors.initials: H
authors.lastname: Yoshizaki
authors.forename: H
authors.initials: H
authors.lastname: Yasuno
authors.forename: T
authors.initials: T
authors.lastname: Sano
authors.forename: J
authors.initials: J
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Yokose
authors.forename: H
authors.initials: H
authors.lastname: Seto
mesh_terms: D000803:Angiotensin I / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011863:Radioimmunoassay; D012988:Soil Microbiology; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013302:Streptomyces / Q000145:classification / Q000378:metabolism*; D013844:Thiazoles / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000092502:Renin Inhibitors
chemical_list: D000803:Angiotensin I; D010456:Peptides, Cyclic; D013844:Thiazoles; D000092502:Renin Inhibitors; C069913:cyclothiazomycin