title: Low access resistance perforated patch recordings using amphotericin B.
abstract: We present experimental procedures describing the creation of perforated patches by use of amphotericin B. In 13 different cellular preparations, access resistances below 10 M omega were achieved and with blunt electrode tips, access resistances of 3-4 M omega were possible. In addition to using the techniques to measure whole cell currents, we have used them to measure single channel currents in a new "outside-out patch" preparation and we have utilized them to measure the resting voltage of epithelial monolayers. We conclude that these new approaches can provide a substantial increase in versatility and quality for many kinds of electrophysiological measurements.
authors.affiliation: Department of Physiology and Biophysics, Mayo Foundation, Rochester, MN 55905.
authors.forename: J
authors.initials: J
authors.lastname: Rae
authors.forename: K
authors.initials: K
authors.lastname: Cooper
authors.forename: P
authors.initials: P
authors.lastname: Gates
authors.forename: M
authors.initials: M
authors.lastname: Watsky
mesh_terms: D000666:Amphotericin B / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D001001:Anura; D002478:Cells, Cultured; D004566:Electrodes; D004594:Electrophysiology / Q000295:instrumentation*; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology; D005399:Fishes; D006168:Guinea Pigs; D006801:Humans; D007908:Lens, Crystalline / Q000502:physiology; D051379:Mice; D009761:Nystatin / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D012996:Solutions; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D012996:Solutions; D009761:Nystatin; D000666:Amphotericin B