title: The frequency of LPS-responsive B cells to autologous and heterologous thyroglobulin.
abstract: The frequency of precursors within the mouse splenic B cell pool, reactive with mouse thyroglobulin (mTg) was estimated using a limiting dilution assay system. The mean frequency was found to be 1/3900 B cells. The results provide a minimal estimate of the frequency of mTg-reactive B cells. The frequency of mTg-reactive B cells was not influenced by the MHC locus, as both high- and low-responder strains showed similar frequencies. While the frequency of B cells reactive to human Tg was found to be similar to that reactive to mTg, only 20% of the mTg-reactive clones also cross-react with human Tg. Similarly, only 30% of huTg reactive clones were found to react with mTg. Therefore, a large proportion of Tg-reactive antibodies are restricted to self-determinants and not determinants to conserved regions of the Tg molecule.
authors.affiliation: Johns Hopkins School of Hygiene and Public Health, Baltimore, Maryland 21205.
authors.forename: R C
authors.initials: RC
authors.lastname: Kuppers
mesh_terms: D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D013045:Species Specificity; D013154:Spleen / Q000166:cytology; D013954:Thyroglobulin / Q000276:immunology*
chemical_list: D008070:Lipopolysaccharides; D013954:Thyroglobulin