Amelogenin post-secretory processing during biomineralization in the postnatal mouse molar tooth.
The primary structures, molecular genetics and biosynthesis of the amelogenin protein of the developing tooth are established, but knowledge of their subsequent post-secretory processing and its relation to enamel biomineralization is fragmentary. Preparations of tooth matrix proteins were isolated from molars (M1) of mice from birth to 15 days and analysed by SDS-PAGE and immunochemical methods. Amelogenin proteins, isolated and partially purified by HPLC, were characterized by amino acid analysis and SDS-PAGE. At birth a 26 kDa amelogenin was present that during subsequent developmental stages generated a series of 20-25 kDa amelogenins differing in apparent size by approximately 1 kDa. Amino acid analyses showed that all these amelogenins have amino-terminal TRAP sequences; analyses for both glycosylation and phosphorylation were negative. It is suggested that these post-secretory amelogenins are generated by a sequence of specific carboxy-terminal cleavages, and that the observed post-secretory processing of amelogenin is functionally linked to the structure of the enamel matrix and the control of crystallite development.
Fincham
A G
AG
Center for Craniofacial Molecular Biology, School of Dentistry, University of Southern California, Los Angeles 90033.
Hu
Y
Y
Lau
E C
EC
Slavkin
H C
HC
Snead
M L
ML
D000367:Age Factors; D000566:Amelogenesis / Q000502:physiology*; D053523:Amelogenin; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003743:Dental Enamel / Q000737:chemistry / Q000502:physiology*; D003746:Dental Enamel Proteins / Q000032:analysis / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis; D051379:Mice; D008963:Molar; D008970:Molecular Weight; D010750:Phosphoproteins / Q000032:analysis; D014074:Tooth Calcification / Q000502:physiology*; D014083:Tooth Germ / Q000737:chemistry / Q000502:physiology*
D053523:Amelogenin; C507200:Amelx protein, mouse; D000596:Amino Acids; D003746:Dental Enamel Proteins; D006023:Glycoproteins; D010750:Phosphoproteins