[Determination of two major metabolites of A-OT-Fu in mice serum by 4th order derivative UV spectrophotometry].
A 4th order derivative UV spectrophotometric method was developed for the determination of two major metabolites (TFu and 5-Fu) of A-OT-Fu in mice serum following per os of 290 mg/kg of the drug. TFu and 5-Fu were extracted from mice serum by ethyl acetate. The amplitudes of 252 and 289 nm for axle are linear with concentration of TFu and 5-Fu within the range of 5-50 micrograms/ml. Under the given experimental condition the recovery of TFu and 5-Fu were over 95%. The maximum concentration of TFu and 5-Fu appeared at 1 h and 2 h respectively after administration.
Zhang
J R
JR
Department of Pharmacy, Shandong Medical University, Jinan.
Wang
W H
WH
Pang
H
H
Cheng
X M
XM
Zhang
C L
CL
D000818:Animals; D000970:Antineoplastic Agents; D005472:Fluorouracil / Q000031:analogs & derivatives* / Q000097:blood; D008297:Male; D051379:Mice; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D000970:Antineoplastic Agents; D005472:Fluorouracil