title: rRNA probing of chromosomal DNA of epidemic and sporadic isolates of Salmonella enterica subsp. enterica serovar Kottbus from northern and southern Italy.
abstract: Fifty-two strains of Salmonella enterica subsp. enterica serovar Kottbus, identified at the Centres of Enterobacteriaceae of Northern and Southern Italy, were investigated by molecular genetic methods. Thirteen isolates were recovered during two food-poisoning outbreaks that occurred in May 1987 in Lombardy. The rDNA gene restriction patterns, obtained by probing endonuclease cleaved chromosomal DNA with photobiotin labeled Escherichia coli rRNA, revealed some heterogeneity among strains isolated from Southern Italy, whereas Northern Italy isolates exhibited virtually identical banding patterns.
authors.affiliation: Istituto di Igiene, Università di Palermo, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Nastasi
authors.forename: C
authors.initials: C
authors.lastname: Mammina
authors.forename: M R
authors.initials: MR
authors.lastname: Villafrate
authors.forename: M
authors.initials: M
authors.lastname: Pontello
authors.forename: M
authors.initials: M
authors.lastname: Corbellini
mesh_terms: D015139:Blotting, Southern; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis*; D004196:Disease Outbreaks; D004926:Escherichia coli / Q000235:genetics; D014644:Genetic Variation; D006801:Humans; D007558:Italy / Q000453:epidemiology; D009693:Nucleic Acid Hybridization; D011153:Population; D015347:RNA Probes; D012335:RNA, Ribosomal / Q000032:analysis*; D012475:Salmonella / Q000235:genetics / Q000302:isolation & purification*; D012478:Salmonella Food Poisoning / Q000453:epidemiology*
chemical_list: D004269:DNA, Bacterial; D015347:RNA Probes; D012335:RNA, Ribosomal