6-Deoxy-6-bromo-ascorbic acid inhibits growth of mouse melanoma cells.
The influence of 6-deoxy-6-bromo-ascorbic acid (6-Br-AA) and ascorbic acid (AA) on the growth of mouse melanoma cell line B16, mouse fibroblasts (L929), and human cervical carcinoma HeLa cells was examined by in vitro experiments. 6-Br-AA was added in concentrations 10(-1) to 10(-8)M and for incubation periods of 2, 18, 24 and 72 h. The present results indicate that 6-Br-AA exhibits a highly pronounced inhibiting effect on growth and DNA synthesis of melanoma cells. Inhibitory effect of 6-Br-AA was confirmed by the in vivo experiments. The tumor-suppressing effect on solid melanoma B16 was attained with 9 mg per mouse given three times daily for 16 days. Our preliminary results indicate that 6-Br-AA could serve as a potential antitumor agent.
Osmak
M
M
Ruder Bosković Institute, Zagreb, Yugoslavia.
Eckert-Maksić
M
M
Pavelić
K
K
Maksić
Z B
ZB
Spavénti
R
R
Beketić
L
L
Kovacek
I
I
Susković
B
B
D000818:Animals; D000970:Antineoplastic Agents; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D008297:Male; D008546:Melanoma, Experimental / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL
D000970:Antineoplastic Agents; C068572:6-deoxy-6-bromoascorbic acid; D001205:Ascorbic Acid