[Segments of 18S rRNA, located in the area of the mRNA-binding center of ribosomes from human placenta].
The affinity labelling of human placenta 80S ribosomes by 4-(N-2-chloroethyl-N-methylamino)benzyl-5'-phosphoramide of hexauridylate has been studied. This mRNA analogue has normal coding properties because its binding to placenta ribosomes significantly increases in the presence of the cognate tRNA(Phe). Incubation of the mRNA analogue in the complex with ribosomes and Phe-tRNAPhe) leads to its covalent attachment exclusively to the small subunit (mainly to 18S rRNA). The reaction site has been shown by hybridisation experiments to be located within positions 975-1055 of 18S rRNA. The identified fragment is located in a highly conserved part of the small subunit rRNA domain II.
Graĭfer
D M
DM
Zenkova
M A
MA
Malygin
A A
AA
Mamaev
S V
SV
Mundus
D A
DA
Karpova
G G
GG
D000345:Affinity Labels; D001483:Base Sequence; D015152:Blotting, Northern; D005260:Female; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism*; D012337:RNA, Ribosomal, 18S / Q000378:metabolism*; D012360:RNA, Transfer, Phe / Q000378:metabolism; D015183:Restriction Mapping
D000345:Affinity Labels; D012333:RNA, Messenger; D012337:RNA, Ribosomal, 18S; D012360:RNA, Transfer, Phe
Uchastok 18S rRNK, lokalizovannyĭ v oblasti mRNK-sviazyvaiushchego tsentra ribosom iz platsenty cheloveka.