Use of metal chelate affinity chromatography for removal of zinc ions from alkaline phosphatase from Escherichia coli.
Alkaline phosphatase from Escherichia coli (APEC) is not retained at 4 degrees C on a metal-free tris(carboxymethyl)ethylenediamine (TED) column, but at 15 degrees C the metalloenzyme becomes bound to the gel. Chromatography of phosphatase on metal-free TED gel indicates a decline in its enzymic activity and zinc content to about 26% and 40%, respectively. The activity of chromatographed APEC can be partially restored by addition of zinc ions, indicating that metal-free TED gel is capable of removing zinc ions from alkaline phosphatase.
Lubińska
V K
VK
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw.
Muszyńska
G
G
D000469:Alkaline Phosphatase / Q000302:isolation & purification* / Q000378:metabolism; D002846:Chromatography, Affinity; D003956:Dialysis; D004793:Enzyme Reactivators; D004926:Escherichia coli / Q000201:enzymology*; D012965:Sodium Chloride; D015032:Zinc / Q000302:isolation & purification* / Q000494:pharmacology
D004793:Enzyme Reactivators; D012965:Sodium Chloride; D000469:Alkaline Phosphatase; D015032:Zinc