title: [3H]-13-cis-retinoic acid covalently binds to thioredoxin reductase in human keratinocytes.
abstract: 13-cis-Retinoic acid is a stereospecific suicide inhibitor of thioredoxin reductase. [3H]-labeled 13-cis-retinoic acid has been used to covalently label thioredoxin reductase in human keratinocytes. The acid-soluble cytosol fraction of human keratinocytes contained three radioactive proteins labeled by the addition of high-specific-activity [3H]-13-cis-retinoic acid to cell cultures. One of these proteins was identified as cytosolic keratinocyte thioredoxin reductase by fast-protein liquid chromatography and SDS-gel radioautography. The inhibition of thioredoxin reductase by 13-cis-retinoic acid may explain the known cytostatic and teratogenic properties attributed to this retinoid.
authors.affiliation: Department of Dermatology, University of Hamburg, FRG.
authors.forename: K U
authors.initials: KU
authors.lastname: Schallreuter
authors.forename: T
authors.initials: T
authors.lastname: Grebe
authors.forename: M R
authors.initials: MR
authors.lastname: Pittelkow
authors.forename: J M
authors.initials: JM
authors.lastname: Wood
mesh_terms: D002478:Cells, Cultured; D002853:Chromatography, Liquid; D006801:Humans; D015603:Keratinocytes / Q000201:enzymology / Q000378:metabolism*; D011485:Protein Binding; D013880:Thioredoxin-Disulfide Reductase / Q000378:metabolism*; D014212:Tretinoin / Q000378:metabolism*; D014316:Tritium
chemical_list: D014316:Tritium; D014212:Tretinoin; D013880:Thioredoxin-Disulfide Reductase