title: Isolation and characterization of the gene encoding a novel, thermostable serine proteinase from the mould Tritirachium album Limber.
abstract: A number of proteinases are induced and secreted into the culture medium of Tritirachium album Limber when the nitrogen source is limited to exogenous proteins. We have constructed a cDNA library using the polyadenylated RNA isolated during the nutritional induction with bovine serum albumin. A full-length clone of a gene for a new proteinase (named proteinase R) was identified from this library. This clone contained sequences coding for the 108-amino-acid prepro-leader as well as for the 279-amino-acid mature proteinase. Proteinase R apparently belongs to the subtilisin group of serine proteases that contains disulphide bonds. Homology between proteinase R and proteinase K was found to be about 87% at the nucleotide as well as at the amino acid level. The Brookhaven Protein Data Base co-ordinate file of proteinase K was used as a template to study the proteinase R substitutions in three-dimensional space. The majority of the substitutions of proteinase R with respect to proteinase K were found to be on the exterior of the protein model.
authors.affiliation: Amgen, Inc., Department 221, Thousand Oaks, California 91320.
authors.forename: B B
authors.initials: BB
authors.lastname: Samal
authors.forename: B
authors.initials: B
authors.lastname: Karan
authors.forename: T C
authors.initials: TC
authors.lastname: Boone
authors.forename: T D
authors.initials: TD
authors.lastname: Osslund
authors.forename: K K
authors.initials: KK
authors.lastname: Chen
authors.forename: Y
authors.initials: Y
authors.lastname: Stabinsky
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D005658:Fungi / Q000201:enzymology* / Q000235:genetics; D005800:Genes, Fungal; D006358:Hot Temperature; D008969:Molecular Sequence Data; D012331:RNA, Fungal / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D012697:Serine Endopeptidases / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D012331:RNA, Fungal; D012697:Serine Endopeptidases