[The participation of protein synthesis in the K+-dependent activation of hormonal biosynthesis in the adrenal cortex of guinea pigs].
An in vitro effect of cycloheximide (CHI), an inhibitor of protein synthesis, on the stimulation of steroidogenesis, caused by a rise of K+ concentration in a medium from 1 to 10 mM, was studied. A rise of a K+ level in the incubation of guinea-pig adrenocortical sections resulted in enhanced protein synthesis and activation of aldosterone and corticosterone synthesis from cholesterol. CHI addition completely suppressed these effects of K+. Comparison of incorporation of a cholesterol and corticosterone mark in aldosterone suggests that a rise of a K+ level within physiological limits enhanced hormone biosynthesis at the stage of splitting off a lateral cholesterol chain. The authors discuss a possible involvement of protein synthesis, stimulated by a high K+ concentration, in acceleration of final reactions of aldosterone biosynthesis.
Chelnakova
I S
IS
Pushkarev
V M
VM
Tron'ko
N D
ND
Mikosha
A S
AS
D000302:Adrenal Cortex / Q000187:drug effects / Q000378:metabolism*; D000305:Adrenal Cortex Hormones / Q000032:analysis / Q000096:biosynthesis*; D000818:Animals; D002855:Chromatography, Thin Layer / Q000379:methods; D003513:Cycloheximide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D006168:Guinea Pigs; D008297:Male; D011188:Potassium / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000187:drug effects; D014316:Tritium
D000305:Adrenal Cortex Hormones; D011506:Proteins; D014316:Tritium; D003513:Cycloheximide; D011188:Potassium
Uchastie belkovogo sinteza v K+-zavisimoĭ aktivatsii biosinteza gormonov v kore nadpochechnikov morskikh svinok.