title: Antibodies to gut protozoa in commercial immunoglobulin preparations.
abstract: Using parasite agglutination, indirect immunofluorescence, enzyme-linked immunosorbent assay, and immunoblotting, Giardia lamblia specific antibody was detected in 5 commercially available immunoglobulin (IgG) preparations. IgG antibodies to Entamoeba histolytica were either present in very low titre or were absent. Immunoblotting showed that anti-G. lamblia antibodies were detected towards a range of Giardia proteins, 25-200 kDa. These findings raise the possibility that pooled human IgG preparations could be evaluated in the treatment of chronic giardiasis which is refractory to conventional therapy.
authors.affiliation: Department of Gastroenterology, St Bartholomew's Hospital, West Smithfield, London, UK.
authors.forename: A M
authors.initials: AM
authors.lastname: Cevallos
authors.forename: P D
authors.initials: PD
authors.lastname: Butcher
authors.forename: A S
authors.initials: AS
authors.lastname: Arvind
authors.forename: A D
authors.initials: AD
authors.lastname: Webster
authors.forename: M J
authors.initials: MJ
authors.lastname: Farthing
mesh_terms: D000371:Agglutination; D000818:Animals; D000913:Antibodies, Protozoan / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D004748:Entamoeba histolytica / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D005872:Giardia / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000276:immunology / Q000302:isolation & purification / Q000627:therapeutic use*
chemical_list: D000913:Antibodies, Protozoan; D007074:Immunoglobulin G