The intramyocardial fate of [1-14C] palmitate, iodopalmitate and iodophenylpentadecanoate in isolated rat hearts. A contribution to the choice of an iodinated fatty acid as a tracer of myocardial metabolism.
Labeled iodinated fatty acids (FAs) have been proposed to explore myocardial metabolism by external detection in man. We have chosen a 16-carbon FA, iodinated in omega position, whereas other authors use an iodophenylated FA. To explore the influence of the presence of an iodine or of an iodophenyl radical on the metabolism of the FA, we have compared, in isolated rat hearts perfused in a recirculating system, the intramyocardial fate of palmitate (PA), iodopalmitate (IPA), and iodophenylpentadecanoate (IPPA), the 3 of them being labeled with C14 in position 1. The addition of the iodine atom brings about a hindrance to the esterification of the FA into triglycerides, but not modification of the myocardial uptake and of the CO2 produced. The addition of the iodophenyl radical impairs both the FA storage and its oxidation, leading to a very high level of free FA. The phospholipid distribution is also modified. Apart from their myocardial use in the isolated rat heart, the 3 FAs were assayed in vitro as a substrate for acylCoA-synthase. As IPA more closely mimics native FA metabolism, it is therefore more suitable than IPPA as a tracer of myocardial metabolism.
Keriel
C M
CM
Laboratoire de Physiologie Cellulaire Cardiaque, Université Joseph Fourier Grenoble, France.
Humbert
T G
TG
Berard
C R
CR
Marti Batlle
D S
DS
Le Bars
D
D
Mathieu
J P
JP
Luu-Duc
C
C
Comet
M
M
Cuchet
P J
PJ
D000818:Animals; D002250:Carbon Radioisotopes; D003066:Coenzyme A Ligases / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D007462:Iodobenzenes / Q000493:pharmacokinetics*; D009206:Myocardium / Q000378:metabolism*; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000493:pharmacokinetics*; D051381:Rats; D011919:Rats, Inbred Strains
D002250:Carbon Radioisotopes; D007462:Iodobenzenes; D010169:Palmitic Acids; D019308:Palmitic Acid; C025391:omega-(4-iodophenyl)pentadecanoic acid; D003066:Coenzyme A Ligases