Effects of gossypol on phorbol ester-calcimycin-induced prostaglandin synthesis by macrophages.
Arachidonic acid metabolism via cyclooxygenase pathway in mouse resident peritoneal macrophages were stimulated by phorbol-12-myristate-13-acetate (PMA), calcimycin and exogenous arachidonic acid, respectively. Racemic, (-) and (+)-gossypol dose-dependently inhibited PMA or calcimycin-induced synthesis of prostacyclin, thromboxane A2 and prostaglandin F2 alpha, but failed to affect arachidonic acid-induced synthesis of prostaglandin. It is suggested that both (-)-gossypol and (+)-gossypol inhibit synthesis of prostaglandin at the level of arachidonic acid release, and the inhibition of prostaglandin synthesis by gossypol might not be the main mode of its antifertility action.
Xiao
D M
DM
Department of Pathophysiology, Peking Union Medical College, Beijing.
Wang
X L
XL
Zhang
J B
JB
Chen
H C
HC
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis*; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology*; D015237:Dinoprost / Q000096:biosynthesis*; D005260:Female; D006072:Gossypol / Q000494:pharmacology*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013929:Thromboxane B2 / Q000096:biosynthesis*
D000001:Calcimycin; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D015237:Dinoprost; D006072:Gossypol; D013755:Tetradecanoylphorbol Acetate