[Effect of ethonium on the lysability of staphylococcal cells by an enzyme preparation of the strain Streptomyces recifensis subsp. lyticus].
The combined effect of ethonium, a synthetic surface active substance and preparations of lytic enzymes on staphylococcal cells and possible use of ethonium for preprocessing the staphylococcal biomass were studied. With addition of ethonium to the reaction system the activity of the enzyme preparations against both the intact staphylococcal cells and the preheated ones markedly increased. The maximum activity of the lytic enzymes was observed when the substance concentration was equal to 25 mg/l. Preprocessing of the biomass with 0.05% ethonium solution increased the rate and strength of the enzymatic lysis. The data are indicative of possible decrease of the concentration of the lytic enzymes and efficient usage of low active preparations in disintegration of staphylococcal cells in the presence of ethonium.
Zhernosekova
I V
IV
Kukushkina
N V
NV
Babenko
Iu S
IuS
D002473:Cell Wall / Q000187:drug effects; D006868:Hydrolysis; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D013210:Staphylococcus / Q000187:drug effects* / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology*; D013501:Surface-Active Agents / Q000494:pharmacology*; D013696:Temperature
D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; C004921:Ethonium
Vliianie étoniia na liziruemost' kletok stafilokokka fermentnym preparatom shtamma Streptomyces recifensis subsp. lyticus.