title: Three regions of erythrocyte band 3 protein are phosphorylated on tyrosines: characterization of the phosphorylation sites by solid phase sequencing combined with capillary electrophoresis.
abstract: The major part of band 3 phosphorylation was recently shown to concern the first tryptic peptide of the protein (Yannoukakos et al. (1991) Biochim. Biophys. Acta 1061, 253-266). Tyrosine 8 is the prevalent site of phosphorylation, but other phosphorylated regions were found which could not be analyzed with certainty. Direct characterization of the phosphorylated residues in all these phosphorylated fragments was made possible due to recent advances in protein chemistry techniques, such as solid phase sequence analysis and capillary electrophoresis. The present report establishes that band 3 phosphorylation occurs predominantly on tyrosines: besides tyrosine 8 already known in the N-terminal region, two other tyrosines are demonstrated to be targets for the tyrosine kinase, tyrosine 359 and tyrosine 904. These residues lie in regions of band 3 exposed to the cytoplasm, the junction of the cytoplasmic and the membrane-spanning domains, and the C-terminal end of the protein which is also cytosolic, respectively.
authors.affiliation: INSERM U299, Hôpital de Bicêtre, Kremlin-Bicêtre, France.
authors.forename: D
authors.initials: D
authors.lastname: Yannoukakos
authors.forename: H E
authors.initials: HE
authors.lastname: Meyer
authors.forename: C
authors.initials: C
authors.lastname: Vasseur
authors.forename: C
authors.initials: C
authors.lastname: Driancourt
authors.forename: H
authors.initials: H
authors.lastname: Wajcman
authors.forename: E
authors.initials: E
authors.lastname: Bursaux
mesh_terms: D000595:Amino Acid Sequence; D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004586:Electrophoresis; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010766:Phosphorylation; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D001457:Anion Exchange Protein 1, Erythrocyte; D014443:Tyrosine