title: New enzyme-linked immunosorbent assay methods for measurement of serum erythropoietin levels and erythropoietin antibodies.
abstract: For clinical studies with erythropoietin (EPO), enzyme-linked immunosorbent assays for the determination of EPO and EPO antibodies were developed. Using polyclonal and monoclonal EPO antibodies in a sandwich technique, serum EPO levels greater than 10 pg/ml (corresponding to 1 mU/ml, calibrated with the 2nd WHO IRP EPO) can be determined. In 103 healthy blood donors, a mean (+/- SD) value of 36 +/- 19 pg EPO/ml was found. Very high EPO concentrations were found in patients suffering from myelodysplastic syndrome and aplastic anemia; elevated levels were associated with rheumatoid arthritis and myelomatosis. No EPO antibodies were detectable in EPO-treated patients.
authors.affiliation: Boehringer-Mannheim GmbH, Tutzing, FRG.
authors.forename: R
authors.initials: R
authors.lastname: Kientsch-Engel
authors.forename: K
authors.initials: K
authors.lastname: Hallermayer
authors.forename: A
authors.initials: A
authors.lastname: Dessauer
authors.forename: L
authors.initials: L
authors.lastname: Wieczorek
mesh_terms: D000906:Antibodies / Q000032:analysis*; D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004921:Erythropoietin / Q000097:blood* / Q000276:immunology; D006402:Hematologic Diseases / Q000097:blood* / Q000276:immunology; D006801:Humans; D011863:Radioimmunoassay
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D004921:Erythropoietin