Protein kinase C mobilization in B lymphocytes. Differential isoenzyme translocation upon activation.
The subcellular distribution of protein kinase C has been analyzed in murine B lymphocytes exposed to LPS, anti-IgM antibodies and phorbol dibutyrate. An accurate determination of the enzyme mobilized from the soluble to the particulate fractions by these activators, has been made possible by the use of B cells in which the major part of the activity was present in the cytosol. Upon stimulation, we have analyzed the isoenzymatic forms translocated to the B cell membrane, showing a differential pattern of isoenzyme mobilization between LPS and anti-IgM antibodies. These data, together with the different Ca2+ requirements for the activation of the translocated protein kinase C isoenzymes, might help to unravel the mechanism responsible for the clonal expansion and differentiation of B lymphocytes, induced by the two ligands.
Márquez
C
C
Centro de Biología Molecular, Universidad Autónoma, Madrid, Spain.
Martínez
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C
Boscá
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L
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000201:enzymology*; D015153:Blotting, Western; D002118:Calcium / Q000502:physiology; D002451:Cell Compartmentation; D002454:Cell Differentiation; D003600:Cytosol / Q000201:enzymology; D004789:Enzyme Activation / Q000187:drug effects; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000378:metabolism*; D008050:Lipid A / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008566:Membranes / Q000201:enzymology; D051379:Mice; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D011493:Protein Kinase C / Q000276:immunology / Q000378:metabolism*; D011947:Receptors, Antigen, B-Cell / Q000502:physiology
D007527:Isoenzymes; D008050:Lipid A; D008070:Lipopolysaccharides; D011947:Receptors, Antigen, B-Cell; D015240:Phorbol 12,13-Dibutyrate; D011493:Protein Kinase C; D002118:Calcium