[Oxidase activity of horseradish peroxidase in oxidation of the bilirubin-albumin complex].
The catalytic activity of horseradish peroxidase was investigated upon oxidation of the bilirubin-albumin complex (10(-5)-10(-4) M; BR: SA = 1: 1.5 mol/mol) by dissolved oxygen to biliverdin and other components. The process can be described by the Michaelis-Menten equation with Km = 14.4 microM and Vmax = 0.12 microM/min, if HRP = 3.1 x 10(-8) M. Oxidation is supposed to proceed in the triple complex--peroxidase: albumin: bilirubin.
Zefirova
O N
ON
Sytov
G A
GA
Postnikov
V A
VA
Valuev
L I
LI
Plate
A
A
D000818:Animals; D001663:Bilirubin / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D002417:Cattle; D006735:Horseradish Peroxidase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D010084:Oxidation-Reduction; D012709:Serum Albumin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D012709:Serum Albumin; D006735:Horseradish Peroxidase; D002374:Catalase; D001663:Bilirubin
Oksidaznaia aktivnost' peroksidazy khrena v okislenii kompleksa bilirubin-al'bumin.