title: Distribution of mouse interferon-beta in normal and brain tumour-bearing mice.
abstract: The distribution of 125I-labelled recombinant mouse interferon-beta (rMuIFN-beta) in normal and glioma (203 glioma) bearing mice was studied by radioassay and macro-autoradiography at 15 and 30 min after a single intravenous injection. The level of rMuIFN-beta in the spleen was about 20-fold higher than in serum. Concentrations higher than the serum level was detected in the lung, liver and kidney. The concentration of rMuIFN-beta in the brain was 8% of the serum level and the concentration in the glioma 30 min after administration was about 10-fold higher than in normal mouse brain. Macro-autoradiographic study demonstrated a wide distribution range and selective uptake in glioma tissue. Furthermore, we found that mouse gliomas were sensitive to mouse IFN-beta. Our findings demonstrate that in the mouse glioma model, intravenously administered interferon reaches the tumour.
authors.affiliation: Department of Neurosurgery, Kumamoto University Medical School, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Mihara
authors.forename: J
authors.initials: J
authors.lastname: Kuratsu
authors.forename: S
authors.initials: S
authors.lastname: Takaki
authors.forename: K
authors.initials: K
authors.lastname: Hori
authors.forename: E
authors.initials: E
authors.lastname: Nagai
authors.forename: Y
authors.initials: Y
authors.lastname: Satoh
authors.forename: N
authors.initials: N
authors.lastname: Minowa
authors.forename: Y
authors.initials: Y
authors.lastname: Ushio
mesh_terms: D000818:Animals; D001345:Autoradiography; D001932:Brain Neoplasms / Q000276:immunology* / Q000628:therapy; D002460:Cell Line; D005910:Glioma / Q000276:immunology* / Q000628:therapy; D007262:Infusions, Intravenous; D007370:Interferon Type I / Q000008:administration & dosage / Q000493:pharmacokinetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D011994:Recombinant Proteins
chemical_list: D007370:Interferon Type I; D011994:Recombinant Proteins