Accuracy of electronic deposition of cells onto microscope slides using a cytometric positioning system.
The Cell Deposition System (CDS) analyzes and deposits biological cells to specific locations on microscope slides. This research will determine the accuracy of the CDS by comparing the stored computer data with the cells placement on the microscope slide. Two independent maps (Control 1 and Control 2) of cell placement for each slide were made, each map to provide a control for the other. The fluorescence data (cell sequence data) was compared to each of the control maps. For the slide, CT4, the error between maps was 13.6% mismatches, between Control 2 and the fluorescence data 32.1% mismatches. Comparing the maps 3/16 rows were perfectly matched; Whereas, comparing fluorescence data to maps 1/16 express perfect matches. Depositing cells in this manner is remarkable accurate. Nevertheless work remains to prevent the "disappearance" of cells, their adjacent positional invasion, and their clustering. This technique provides a superior preparative method for sample archival storage and for further automated multiparametric microscope assessment.
Jalbuena
R C
RC
Dept. of Elect. and Computer Eng., Univ. of Missouri, Columbia.
Tyrer
H W
HW
D000818:Animals; D002469:Cell Separation; D002645:Chickens; D004912:Erythrocytes / Q000166:cytology; D005434:Flow Cytometry / Q000379:methods*; D011898:Ranidae