title: Neonatal lethality and lymphopenia in mice with a homozygous disruption of the c-abl proto-oncogene.
abstract: The c-abl proto-oncogene, which encodes a cytoplasmic protein-tyrosine kinase, is expressed throughout murine gestation and ubiquitously in adult mouse tissues. However, its levels are highest in thymus, spleen, and testes. To examine the in vivo role of c-abl, the gene was disrupted in embryonic stem cells, and the resulting genetically modified cells were used to establish a mouse strain carrying the mutation. Most mice homozygous for the c-abl mutation became runted and died 1 to 2 weeks after birth. In addition, many showed thymic and splenic atrophy and a T and B cell lymphopenia.
authors.affiliation: Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142.
authors.forename: V L
authors.initials: VL
authors.lastname: Tybulewicz
authors.forename: C E
authors.initials: CE
authors.lastname: Crawford
authors.forename: P K
authors.initials: PK
authors.lastname: Jackson
authors.forename: R T
authors.initials: RT
authors.lastname: Bronson
authors.forename: R C
authors.initials: RC
authors.lastname: Mulligan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015139:Blotting, Southern; D003001:Cloning, Molecular; D005804:Genes, Lethal; D005818:Genetic Engineering; D006410:Hematopoiesis; D006579:Heterozygote; D006720:Homozygote; D008231:Lymphopenia / Q000235:genetics; D051379:Mice; D008817:Mice, Mutant Strains; D016315:Proto-Oncogene Proteins c-abl / Q000502:physiology*; D011519:Proto-Oncogenes; D011995:Recombination, Genetic; D013154:Spleen / Q000002:abnormalities; D013950:Thymus Gland / Q000002:abnormalities
chemical_list: D016315:Proto-Oncogene Proteins c-abl