The association of "standard ELISA" and HIV (env) ELISA for detection and confirmation of HIV antibodies in human sera.
Sera from various population of subjects, including patients with AIDS and ARC, drug-addicts seropositive for HIV and healthy blood donors were screened with "standard ELISA" and HIV (env) ELISA. In the present study all 80 specimens from patients with AIDS and all 60 patients with ARC were positive by HIV (env) ELISA, and 1507 specimens from HIV drug positive by W.B. were detected as positive by HIV (env) ELISA. The specificity of HIV (env) ELISA was defined in terms of percentage of non-reacting persons in a low risk population is 100%. Furthermore the HIV (env) ELISA is highly specific and sensitive and could be used in association with "standard ELISA" for detection and confirmation of HIV antibodies in human sera and plasma.
Filice
G
G
IRCCS Policlinico S. Matteo, Department of Infectious Diseases, University of Pavia.
Soldini
L
L
Orsolini
P
P
Campisi
D
D
Chiesa
A
A
Gulminetti
R
R
Achilli
G
G
Cattaneo
E
E
D015492:AIDS Serodiagnosis / Q000379:methods*; D000386:AIDS-Related Complex / Q000276:immunology; D000163:Acquired Immunodeficiency Syndrome / Q000150:complications / Q000276:immunology; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005188:False Negative Reactions; D005189:False Positive Reactions; D015483:HIV Antibodies / Q000097:blood*; D015488:HIV Antigens / Q000276:immunology; D015700:HIV Envelope Protein gp41 / Q000138:chemical synthesis / Q000276:immunology*; D006801:Humans; D012680:Sensitivity and Specificity; D019966:Substance-Related Disorders / Q000150:complications
D000911:Antibodies, Monoclonal; D015483:HIV Antibodies; D015488:HIV Antigens; D015700:HIV Envelope Protein gp41