Microwave-stimulated fixation for electron microscopy using a domestic microwave oven.
We investigated microwave-stimulated fixation of tissues for transmission electron microscopy using a domestic microwave oven operating at a frequency of 2.45 GHz with an output power of 500W. Microwave-stimulated fixation, in 4% glutaraldehyde, of fresh rat kidney, liver, heart and brain tissues was compared to conventional fixation. Human renal biopsies were similarly studied. Electron microscopy showed excellent ultrastructural preservation comparable to that obtained by conventional fixation. The optimal temperature range for microwave-stimulated fixation was found to lie between 50 degrees C and 55 degrees C. Our results indicate that microwave-stimulated fixation is a rapid and reproducible technique and can be effectively applied to routine diagnostic pathology.
Ng
K H
KH
Department of Pathology, University of Malaya, Kuala Lumpur.
Gan
S K
SK
D000818:Animals; D005404:Fixatives; D006652:Histological Techniques / Q000295:instrumentation; D006801:Humans; D008854:Microscopy, Electron; D008872:Microwaves; D051381:Rats
D005404:Fixatives