[Proliferation behavior of the anagen hair bulb in androgenetic alopecia before and after local administration of 17 alpha- propylmesterolone--DNA flow cytophotometry studies].
10 male test persons with androgenetic alopecia (Hamilton stage IV) were topically treated with an alcoholic 17 alpha-propylmesterolone solution (PM, 3%) for 3 months. Before and after treatment, we took deep biopsies from the fronto-temporal scalp region. Microsurgically, we isolated anagen hair bulbs, connective tissue with epidermis, and fat from the biopsy samples in order to determine cell cycle kinetics by means of DNA-flow cytometry. By comparison, cell cycle kinetics of the pre- and post-treatment samples did not show any significant changes with regard to connective tissue and fat, whereas in anagen hair bulbs, we found a significant increase of both S-phase cells (%) and the proliferation index (%). These results imply that PM, if topically applied, enhances the proliferative activity of hair matrix cells.
Kiesewetter
F
F
Dermatologische Universitäts-Klinik Erlangen.
Schell
H
H
Seidel
C
C
von Hintzenstern
J
J
D000287:Administration, Topical; D000328:Adult; D000505:Alopecia / Q000188:drug therapy*; D000726:Androgen Antagonists; D002455:Cell Division / Q000187:drug effects*; D004261:DNA Replication / Q000187:drug effects*; D004334:Drug Administration Schedule; D005434:Flow Cytometry; D006197:Hair / Q000187:drug effects* / Q000254:growth & development; D006801:Humans; D008297:Male; D008655:Mesterolone / Q000008:administration & dosage / Q000031:analogs & derivatives*
D000726:Androgen Antagonists; D008655:Mesterolone; C041614:17-propylmesterolone
Proliferationsverhalten des anagenen Haarbulbus bei androgenetischer Alopezie vor und nach lokaler Applikation von 17 alpha-Propylmesterolon--DNS-impulszytophotometrische Untersuchungen.