Ammonia as a potential mediator of adult human periodontal infection: inhibition of neutrophil function.
Neutrophils (polymorphonuclear leucocytes) are the principal cell of the host defence system. Consequently, if periodontal pathogen-derived substances in the gingival crevice significantly inhibit their function, they could shift the bacterial-host balance in favour of the bacteria. The hypothesis that ammonia can inhibit neutrophil function was tested. Ammonia was specifically selected because periodontal pathogens produce substantial amounts of ammonia. The findings indicated that ammonia can inhibit neutrophil phagocytosis, degranulation and oxygen metabolism. Ammonia decreased the total number of phagocytosing polymorphonuclear neutrophils (66% of control) and also decreased degranulation (61% of control). Ammonia decreased oxygen metabolism of both resting and stimulated neutrophils (33 and 42% of control, respectively). These observations support the hypothesis that ammonia can inhibit the function of polymorphonuclear leukocytes. They suggest that the presence of ammonia in the gingival crevice may increase the risk of development of periodontal disease.
Niederman
R
R
Forsyth Research Institute, Department of Cell Biology, Boston, MA 02115.
Brunkhorst
B
B
Smith
S
S
Weinreb
R N
RN
Ryder
M I
MI
D000328:Adult; D000641:Ammonia / Q000494:pharmacology*; D015550:Cell Degranulation / Q000187:drug effects; D003594:Cytoplasmic Granules / Q000201:enzymology; D006801:Humans; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D010101:Oxygen Consumption / Q000187:drug effects; D010196:Pancreatic Elastase / Q000032:analysis / Q000378:metabolism; D010510:Periodontal Diseases / Q000473:pathology*; D010587:Phagocytosis / Q000187:drug effects
D000641:Ammonia; D010196:Pancreatic Elastase