Quantitative reappraisal of general expressions for multivalent protein binding in subunit-exchange chromatography.
Quantitative expressions have been derived for bivalent equilibria with immobilized ligand systems and for the equilibria for an immobilized protein whose self-association is modified by binding with a soluble ligand, as analyzed by affinity chromatography. These general expressions have been applied in a reexamination of multivalency in the affinity chromatography of antibodies, as reported by Eilat and Chaiken (Biochemistry 18 (1979) 790) and also to studies of neurophysin-peptide hormone interactions using glass matrices reported by Swaisgood and Chaiken (Biochemistry 25 (1986) 4148).
Chun
P W
PW
Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville 32610.
Milov
D E
DE
Jou
W S
WS
D001127:Arginine Vasopressin / Q000737:chemistry; D002846:Chromatography, Affinity; D007070:Immunoglobulin A / Q000737:chemistry; D008433:Mathematics; D009481:Neurophysins / Q000737:chemistry; D010767:Phosphorylcholine / Q000737:chemistry; D011506:Proteins / Q000378:metabolism*
D007070:Immunoglobulin A; D009481:Neurophysins; D011506:Proteins; D010767:Phosphorylcholine; D001127:Arginine Vasopressin