title: cDNA cloning of carboxyl ester lipase from human pancreas reveals a unique proline-rich repeat unit.
abstract: We report the isolation and nucleotide sequence of the cDNA for carboxyl ester lipase (CEL) from human pancreas. CEL was purified from human pancreas and microsequence analysis was performed on the amino-terminal and internal peptides. Peptide sequence was used to design oligonucleotide probes for screening a human pancreas cDNA library. Partial length cDNAs for CEL were isolated from the library, and the 5' portion of the cDNA was obtained using the anchored polymerase chain reaction. The deduced amino acid sequence indicates that mature CEL contains 722 amino acids and is synthesized with a 20 amino acid leader peptide. The amino acid sequence is rich in proline (12.2%), with 68% of the proline residues occurring within the final 25% of protein length. This is due to the occurrence of a series of proline-rich tandem repeat units near the carboxyl terminus, and accounts for the previously observed species variation in CEL size and amino acid composition. The primary sequence of CEL shows strong similarity to members of the serine esterase family, including the identical G-E-S-A-G motif at the putative active site. A striking homology also occurs between CEL and acetylcholinesterase and cholinesterase, essential enzymes of the nervous system. Proteins with cholesteryl esterase activity have been detected in extra-pancreatic tissues including liver, intestine, kidney, aorta, macrophage, and in the milk of some species (human, gorilla, cat, dog), but not others (rat, cow). To clarify the structural relationships between these various esterases and CEL, we used the CEL cDNA to study expression in pancreas and liver. CEL mRNA was abundant in pancreas of human and rat, with the human CEL mRNA approximately 300 nucleotides larger than that from rat. CEL mRNA was not detected in human adult or fetal liver, nor in rat liver. These results indicate that CEL is not synthesized in significant amounts in liver, and suggest that the cholesterol esterase activity that has been described in liver may be due to a distinct enzyme, or may be derived from pancreas, as has been proposed for the cholesterol esterase activity in intestine.
authors.affiliation: Lipid Research, VA Wadsworth Medical Center, Los Angeles, CA 90073.
authors.forename: K
authors.initials: K
authors.lastname: Reue
authors.forename: J
authors.initials: J
authors.lastname: Zambaux
authors.forename: H
authors.initials: H
authors.lastname: Wong
authors.forename: G
authors.initials: G
authors.lastname: Lee
authors.forename: T H
authors.initials: TH
authors.lastname: Leete
authors.forename: M
authors.initials: M
authors.lastname: Ronk
authors.forename: J E
authors.initials: JE
authors.lastname: Shively
authors.forename: B
authors.initials: B
authors.lastname: Sternby
authors.forename: B
authors.initials: B
authors.lastname: Borgström
authors.forename: D
authors.initials: D
authors.lastname: Ameis
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000235:genetics* / Q000378:metabolism; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA; D006801:Humans; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D011392:Proline / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D016415:Sequence Alignment
chemical_list: D012333:RNA, Messenger; D004247:DNA; D011392:Proline; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase