[An analysis of the molecular forms of the lipopolysaccharide from Pseudomonas syringae pv. atrofaciens IMV K-1025].
Water extract and salt-EDTA extract of Pseudomonas syringae, pv. atrofaciens cells were fractionated by ultracentrifugation with following salting out of ultracentrifugal supernatant by ammonium sulphate at 55% saturation (pH 4.5). The composition and distribution of LPS molecular forms were studied in the obtained fractions by means of electrophoresis in 10% polyacrylamide gel with 1% sodium dodecylsulphate when staining gels by silver nitrate and cumassi. It is shown that ultracentrifugal supernatant and a sediment as well as sulphate sediment contain S-LPS and R-LPS. SR-LPS is not differentiated. Sulphate supernatant does not contain the determinable amount of S-LPS but it is enriched by the proteins with molecular weights of 65-15 kDalton. S-LPS is localized in the gel area which corresponds to mobilities of polypeptides with molecular weights 130-45 KDalton and the number of monomeric links in O-specific chains of its molecules reaches 25-30. R-LPS migrates under electrophoresis in gel to the mobility zone of polypeptides with molecular weights 14.5-16 kDalton.
Ovod
V V
VV
Mikhal'skiĭ
L A
LA
Vershigora
A E
AE
Gvozdiak
R I
RI
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000302:isolation & purification; D001704:Biopolymers; D004591:Electrophoresis, Polyacrylamide Gel; D008070:Lipopolysaccharides / Q000032:analysis* / Q000302:isolation & purification; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification; D011549:Pseudomonas / Q000032:analysis*; D014461:Ultracentrifugation
D001425:Bacterial Outer Membrane Proteins; D001704:Biopolymers; D008070:Lipopolysaccharides; D010455:Peptides
Analiz molekuliarnykh form lipopolisakharida Pseudomonas syringae pv. atrofaciens IMV K-1025.