Clinical and experimental mycotic keratitis caused by Aspergillus terreus and the effect of subconjunctival oxiconazole treatment in the animal model.
Aspergillus terreus was isolated from a case of Keratomycosis. The patient, a 50 year old, female presented with a large corneal ulcer with hypopyon. The direct microscopic examination of the scrapings revealed hyaline, thin, septate and branched hyphae. In vitro some antimycotics (amphotericin B, 5-fluorocytosine, oxiconazole, amorolfine and ketoconazole) were tested against A. terreus by agar dilution method. Ketoconazole with MIC of 3 micrograms/ml after 7 days of incubation was most effective followed by oxiconazole (10 micrograms/ml). Experimental corneal ulcer was produced by injecting intralamellary 0.1 ml of the spore suspension containing 10 x 10(6) cfu/ml into the eyes of previously immunocompressed albino rabbits. Histopathologic examination showed infiltration and large destruction of the corneal stroma. Subconjunctival oxiconazole therapy exhibited complete cure. Based on our findings, a clinical evaluation of oxiconazole in human keratomycosis seems to be justified.
Singh
S M
SM
Department of Biological Sciences, Rani Durgavati University, Jabalpur, India.
Sharma
S
S
Chatterjee
P K
PK
D000666:Amphotericin B / Q000494:pharmacology; D000818:Animals; D000935:Antifungal Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D001228:Aspergillosis / Q000188:drug therapy* / Q000473:pathology; D001230:Aspergillus / Q000187:drug effects; D003320:Corneal Ulcer / Q000188:drug therapy* / Q000473:pathology; D004195:Disease Models, Animal; D005260:Female; D005437:Flucytosine / Q000494:pharmacology; D006801:Humans; D007093:Imidazoles / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D007654:Ketoconazole / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D009025:Morpholines / Q000494:pharmacology; D011817:Rabbits
D000935:Antifungal Agents; D007093:Imidazoles; D009025:Morpholines; D000666:Amphotericin B; C038974:amorolfine; C022155:oxiconazole; D005437:Flucytosine; D007654:Ketoconazole