CA 19-9 and CA 125 determination by immunoluminometric assay.
We evaluated a new immunoluminometric technique (ILMA) for the measurement of the cancer antigens, CA 19-9 and CA 125 in serum. A satisfactory reproducibility was found, coefficients of variation ranging from 4.2 to 7.3% in within-run and between-run assays. The linearity of tests was maintained over a wide concentration range. Mean analytical recovery was 94% for CA 19-9 and 98% for CA 125. A significant agreement between results obtained by immunoradiometric assays and evaluated methods was found both for CA 125 (ILMA = 0.917 IRMA + 1.048; r = 0.966; n = 98) and CA 19-9 (ILMA = 1.156 IRMA + 0.996; r = 0.995; n = 100).
Plebani
M
M
Cattedra e Servizio di Biochimica Clinica, Università di Padova, Italia.
Faggian
D
D
Masiero
M
M
Giacomini
A
A
Burlina
A
A
D000328:Adult; D000368:Aged; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000097:blood*; D014408:Biomarkers, Tumor / Q000097:blood; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000706:statistics & numerical data; D015592:Immunoradiometric Assay; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D010190:Pancreatic Neoplasms / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D015203:Reproducibility of Results
D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor