title: Assay of hydroxyfarrerol in biological fluids.
abstract: A high-performance liquid chromatographic method for the determination of hydroxyfarrerol (IdB 1031) in biological samples was developed. IdB 1031 was first extracted by liquid-solid partition and the extracts were evaporated and analysed on a reversed-phase column under isocratic conditions, using either an electrochemical or a UV detector. The detection limit was ca. 5 ng/ml. Preliminary pharmacokinetic data showed that rats treated orally with 500 mg/kg had an average peak plasma concentration (Cmax) of 497 ng/ml after 2 h.
authors.affiliation: Drug Metabolism and Pharmacokinetic Department, Sigma-Tau SpA, Rome, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Marzo
authors.forename: E
authors.initials: E
authors.lastname: Arrigoni Martelli
authors.forename: G
authors.initials: G
authors.lastname: Bruno
authors.forename: E M
authors.initials: EM
authors.lastname: Martinelli
authors.forename: G
authors.initials: G
authors.lastname: Pifferi
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000097:blood*; D051381:Rats
chemical_list: D005419:Flavonoids; C068573:IdB 1031