An RME1-independent pathway for sporulation control in Saccharomyces cerevisiae acts through IME1 transcript accumulation.
The RES1-1 mutation was isolated on the basis of its ability to allow MATa/MAT alpha diploid Saccharomyces cerevisiae cells to express a late sporulation-regulated gene, SPR3, in the presence of excess copies of RME1. RME1 is a repressor of meiosis that is normally expressed in cells that lack the a1/alpha 2 repressor encoded by MAT. The RES1-1 mutation also supports sporulation in mat-insufficient diploids. This phenotype does not result from a failure to express RME1 and is not due to activation of the silent copies of mating type information. RES1-1 activates sporulation by allowing IME1 accumulation in all cell types, irrespective of the presence of the MAT products. IME1 is still responsive to RME1 in RES1-1 cells, since double mutants (rme1 RES1-1) that are deficient at MAT can sporulate better than either single mutant. RES1-1 is not an allele of IME1.
Kao
G
G
Department of Biological Sciences, University of New Orleans, Louisiana 70148.
Shah
J C
JC
Clancy
M J
MJ
D000483:Alleles; D015152:Blotting, Northern; D003433:Crosses, Genetic; D004171:Diploidy; D004843:Epistasis, Genetic; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D009154:Mutation; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology; D013172:Spores, Fungal; D014158:Transcription, Genetic