Electrostatic assay of phospholipase A activity: an application of the second harmonic method of monitoring membrane boundary potentials.
To evaluate phospholipase A activity a new assay is suggested. This assay is based on the recording of boundary potential changes of the planar bilayer lipid membrane during enzymatic hydrolysis of lipids. To register these changes, a second harmonic method is used. Sensitivity of the assay is about 0.0002 units/ml regardless of the impurities that may be present in the samples. One analysis takes about 5 min.
Mirsky
V M
VM
Frumkin Institute of Electrochemistry U.S.S.R. Academy of Sciences, Moscow.
Cherny
V V
VV
Sokolov
V S
VS
Markin
V S
VS
D004560:Electricity; D008051:Lipid Bilayers / Q000378:metabolism; D008564:Membrane Potentials / Q000502:physiology; D010741:Phospholipases A / Q000378:metabolism*
D008051:Lipid Bilayers; D010741:Phospholipases A