Application of capillary DNA chromatography to detect AIDS virus (HIV-1) DNA in blood.
The application of capillary DNA chromatography to the detection of the AIDS human immunodeficiency virus, type 1 (HIV-1) proviral DNA in blood is reported for the first time. Combining polymerase chain reaction with fluorescence-labeled DNA probes provides the basis for the amplification and specific detection of the DNA. Samples analyzed for HIV-1 DNA included both infected human blood and cell cultures. The new DNA separation method, capillary DNA chromatography (related to both capillary electrophoresis and capillary hydrodynamic chromatography), is shown to be a powerful method of analysis for DNA. These preliminary results indicate that an automated approach to screening the blood supply for HIV-1 may become a reality in the future.
Brownlee
R G
RG
Irwin Memorial Blood Bank, San Francisco, CA 94118.
Sunzeri
F J
FJ
Busch
M P
MP
D002845:Chromatography / Q000379:methods*; D015342:DNA Probes; D004279:DNA, Viral / Q000097:blood*; D004586:Electrophoresis / Q000379:methods; D005453:Fluorescence; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction
D015342:DNA Probes; D004279:DNA, Viral