Treatment of rat pancreatic islets with MHC Ia antibody and complement prior to transplantation.
Rat pancreatic islets were treated with MHC Ia antigen monoclonal antibody and complement in order to lyse Ia antigen bearing cells within the islets. Ia antigen expression was significantly reduced after this treatment as shown by indirect immunofluorescence tests. Glucose stimulated insulin secretion and viability of the islets were not impaired. The partial reduction of the Ia antigen expression, however, did not provoke a prolongation of the graft acceptance after islet allotransplantation across a major histocompatibility barrier.
Flesch
B K
BK
Medizinische Klinik III, Justis Liebig Universität, Giessen, Germany.
Otto
D A
DA
Bretzel
R G
RG
Federlin
K
K
D000818:Animals; D002470:Cell Survival / Q000502:physiology; D003165:Complement System Proteins / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006082:Graft Enhancement, Immunologic / Q000379:methods*; D006085:Graft Survival / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D016381:Islets of Langerhans Transplantation / Q000276:immunology* / Q000379:methods; D007518:Isoantibodies / Q000276:immunology*; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D014184:Transplantation, Homologous / Q000276:immunology
D000949:Histocompatibility Antigens Class II; D007328:Insulin; D007518:Isoantibodies; D003165:Complement System Proteins