Protective effect of S-nitroso-N-acetyl-penicillamine in endotoxin-induced acute intestinal damage in the rat.
Macroscopic jejunal damage and plasma leakage induced within 15 min by E. coli lipopolysaccharide (LPS 50 mg kg-1 i.v.) in the rat was enhanced by the inhibitor of nitric oxide (NO) formation, NG-monomethyl-L-arginine (L-NMMA 50 mg kg-1 i.v.). The nitro-vasodilator, S-nitroso-N-acetyl-penicillamine (SNAP; 10 micrograms kg-1 min-1 i.v.), which generates NO, attenuated both LPS-induced intestinal damage and the enhancement of such damage and plasma leakage produced by L-NMMA. Endogenous NO may thus have a protective role in the intestinal vasculature that can be mimicked by generators of NO.
Boughton-Smith
N K
NK
Department of Pharmacology, Wellcome Research Laboratories, Beckenham, Kent, U.K.
Hutcheson
I R
IR
Deakin
A M
AM
Whittle
B J
BJ
Moncada
S
S
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects; D004731:Endotoxins / Q000037:antagonists & inhibitors / Q000633:toxicity*; D004926:Escherichia coli; D007410:Intestinal Diseases / Q000139:chemically induced / Q000503:physiopathology / Q000517:prevention & control*; D007583:Jejunum / Q000187:drug effects; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D010396:Penicillamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D026423:S-Nitroso-N-Acetylpenicillamine; D012711:Serum Albumin, Radio-Iodinated; D014665:Vasodilator Agents / Q000494:pharmacology*; D019323:omega-N-Methylarginine
D004731:Endotoxins; D008070:Lipopolysaccharides; D012711:Serum Albumin, Radio-Iodinated; D014665:Vasodilator Agents; D019323:omega-N-Methylarginine; D026423:S-Nitroso-N-Acetylpenicillamine; D001120:Arginine; D010396:Penicillamine