Proteinase-antiproteinase imbalance in meningitis: determination of alpha 1 proteinase inhibitor (alpha 1PI), elastase-alpha 1PI complex, and elastase inhibition capacity in cerebrospinal fluid.
Mortality and long-term neurologic sequelae are still frequent complications of meningitis despite effective antibiotic treatment. This suggests that pathogen-independent inflammatory mechanisms may play an important role in the course of this illness. Neutrophil granulocytes form the primary immune defense in meningitis. Once activated, these cells release elastase into the cerebrospinal fluid (CSF). Elastase may induce tissue damage if local antiproteinase capacity is low as under normal conditions. To define the relevance of this mechanism we studied 22 patients with meningitis. Concentrations of elastase in complex with the main antiproteinase alpha 1-proteinase inhibitor (elastase-alpha 1 PI), alpha 1-proteinase inhibitor (alpha 1PI), and elastase inhibition capacity (EIC) were measured in CSF of 9 patients with bacterial meningitis (BM), aged 1 month-14 years; 13 patients with non-bacterial meningitis (NBM), aged 1 month-15 years; and 20 patients in whom meningitis was excluded after spinal tap (control group), aged 6 months-15 years. The concentration of elastase-alpha 1PI in the BM group (median 552 micrograms/l) was significantly higher than in either the NBM group (median 30 micrograms/l, p less than 0.01) or the control group (median 30 micrograms/l, p less than 0.01). Similarly, the alpha 1PI-concentration in the BM group was significantly higher (median 113 mg/l) than either the NBM group (median 13.7 mg/l, p less than 0.025) or the control group (median 6.3 mg/l, p less than 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)
van Wees
J
J
Klinik für Pädiatrie, Medizinische Universität zu Lübeck.
Tegtmeyer
F K
FK
Otte
J
J
Wood
W G
WG
Braun
J
J
D000293:Adolescent; D001424:Bacterial Infections / Q000134:cerebrospinal fluid / Q000201:enzymology; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007223:Infant; D008581:Meningitis / Q000134:cerebrospinal fluid / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000134:cerebrospinal fluid*; D011480:Protease Inhibitors / Q000134:cerebrospinal fluid; D000515:alpha 1-Antitrypsin / Q000134:cerebrospinal fluid*
D011480:Protease Inhibitors; D000515:alpha 1-Antitrypsin; D010196:Pancreatic Elastase