Mice homozygous for the ablm1 mutation show poor viability and depletion of selected B and T cell populations.
The c-abl gene, originally identified as the cellular homolog of the transforming gene of the Abelson murine leukemia virus, encodes a protein-tyrosine kinase of unknown function that is expressed in all mammalian tissues. We have previously described the introduction of a mutation in the c-abl gene into the mouse germline via targeted gene disruption of embryonic stem cells. We now show that mice homozygous for this mutation are severely affected, displaying increased perinatal mortality, runtedness, and abnormal spleen, head, and eye development. We have examined components of the immune system and have found major reductions in B cell progenitors in the adult bone marrow, with less dramatic reductions in developing T cell compartments.
Schwartzberg
P L
PL
Department of Biochemistry and Molecular Biophysics, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
Stall
A M
AM
Hardin
J D
JD
Bowdish
K S
KS
Humaran
T
T
Boast
S
S
Harbison
M L
ML
Robertson
E J
EJ
Goff
S P
SP
D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D016175:B-Lymphocyte Subsets / Q000166:cytology; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D005124:Eye Abnormalities / Q000235:genetics; D005434:Flow Cytometry; D006410:Hematopoiesis; D006720:Homozygote; D008214:Lymphocytes / Q000166:cytology*; D051379:Mice; D008817:Mice, Mutant Strains; D009841:Oligonucleotides / Q000737:chemistry; D010641:Phenotype; D010766:Phosphorylation; D016133:Polymerase Chain Reaction; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D016315:Proto-Oncogene Proteins c-abl / Q000235:genetics*; D013154:Spleen / Q000166:cytology / Q000473:pathology; D016176:T-Lymphocyte Subsets / Q000166:cytology
D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte; D009841:Oligonucleotides; D011505:Protein-Tyrosine Kinases; D016315:Proto-Oncogene Proteins c-abl