[Immunogenicity and stability of a Soviet inactivated cultured vaccine against Japanese encephalitis].
Six immunologically active vaccine batches inducing a specific antibody to Japanese encephalitis (JE) virus were obtained in serial manufacture of the preparation. In HI tests, the minimal antibody titre was 1:80, the maximal 1:320, neutralization index 1g was 3.7 to 5.2. The data on the stability of the antigenic potency of the vaccines in relation to the duration of storage at 4 degrees-6 degrees C are presented (the follow-up period 3 years). A certain relationship was found between the antigenic potency of the preparation and the titre of the initial infectious tissue culture virus. Also, a definite correlation was found between the initial immunogenic potency of the vaccines and their stability in storage. After 3 years of storage, three vaccine lots remained antigenically active, namely those which after manufacture had induced antihemagglutinins in titres 1:160 to 1:320. The antigenic activity of 6 vaccine batches prepared from the production strain Peking-1 (Nakayama serotype) was studied against the predominant strain of Jagar-10 serotype. All the freshly prepared vaccine batches were found to induce production of antihemagglutinins to both serotypes of JE virus, whereas virus-neutralizing antibodies were found only to the test strain Nakajama-NIH homologous to the vaccine Peking-1 strain. After 1 year of storage, four vaccine batches lost their capacity to induce production of antihemagglutinins to Jagar-01 strain, two batches induced antibody in low titres. This fact should be considered in evaluation of postvaccination immunity status in humans.
Khanina
M K
MK
Bochkova
N G
NG
Grachev
V P
VP
El'bert
L B
LB
Vorob'eva
M S
MS
Borsuk
E A
EA
Pogodina
V V
VV
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology; D004355:Drug Stability; D004356:Drug Storage; D004664:Encephalitis Virus, Japanese / Q000276:immunology*; D006168:Guinea Pigs; D006385:Hemagglutination Inhibition Tests; D051379:Mice; D012692:Serial Passage; D013997:Time Factors; D014613:Vaccines, Attenuated / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification / Q000633:toxicity; D014765:Viral Vaccines / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification / Q000633:toxicity
D000914:Antibodies, Viral; D000956:Antigens, Viral; D014613:Vaccines, Attenuated; D014765:Viral Vaccines
Immunogennost' i stabil'nost' otechestvennoĭ kul'tural'noĭ inaktivirovannoĭ vaktsiny protiv iaponskogo éntsefalita.