ROESY studies and HSEA calculations on xylose-containing oligosaccharides related to N-glycoproteins.
Conformational studies on beta-D-Xyl-(1----2)-[alpha-D-Man-(1----6)]-beta-D-OMe, beta-D-Xyl-(1----2)-[alpha-D-Man-(1----3)]-beta-D-Man-OMe, and beta-D-Xyl-(1----2)-[alpha-D-Man-(1----3)]-[alpha-D-Man-(1----6)]-beta-D -Man-Ome have been carried out using rotating-frame n.O.e. experiments in combination with HSEA calculations. The estimated time-averaged torsional angles phi and psi about the various glycosidic linkages and the estimated time-averaged rotamer population around the C-5-C-6-linkage of beta-Man in the tetrasaccharide are similar to the corresponding parameters for both trisaccharides.
Leeflang
B R
BR
Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.
Bouwstra
J B
JB
Kerékgyártó
J
J
Kamerling
J P
JP
Vliegenthart
J F
JF
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006023:Glycoproteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D012984:Software; D013816:Thermodynamics; D014994:Xylose / Q000737:chemistry*
D006023:Glycoproteins; D009844:Oligosaccharides; D014994:Xylose