Post-column continuous-flow analysis combined with reversed-phase liquid chromatography and computer-aided detection for the characterisation of peptides.
Peptide mapping is a key technique for structural identification of new proteins or the products of recombinant gene technology. The recognition of oligopeptides, separated by reversed-phase liquid chromatography, is limited by the conventional reliance on the correlation of retention times with standards, supported by dual-wavelength chromatograms. It has been reported that the recognition of phenolic compounds can be achieved by a novel technique, based on computer-aided photodiode-array detection of the pH-shifted solutes after post-column continuous-flow analysis. This work describes how the generation of the pH-shifted difference spectra for dipeptides, containing a tyrosyl residue, may be used to enhance peak recognition, when used in conjunction with absorbance ratios.
Fell
A F
AF
Department of Pharmaceutical Chemistry, School of Pharmacy, University of Bradford, U.K.
Castledine
J B
JB
Sellberg
B
B
Modin
R
R
Weinberger
R
R
D015502:Absorptiometry, Photon; D000596:Amino Acids / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D001330:Electronic Data Processing / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D010455:Peptides / Q000737:chemistry*
D000596:Amino Acids; D010455:Peptides