BIS 1, a major component of the cereal genome and a tool for studying genomic organization.
We report the cloning and characterization of an element (BIS 1) in barley. Related sequences were also found in wheat and rye genomes. BIS 1-related sequences may be some of the more frequent of the complex repeats in the barley genome, and their dispersion throughout the barley genome suggests that they are capable of both mobility and amplification. BIS 1 sequences have been used to study the gross structure of the barley genome. These studies indicate that the genome may be formed from larger genomic structures of tens of kilobases which may be repeated. Surprisingly, these studies also show that these large genomic structures also occur in similar proportions and at similar size distribution in the wheat genome.
Moore
G
G
Cambridge Laboratory, John Innes Centre for Plant Science Research, Norwich, Norfolk, United Kingdom.
Cheung
W
W
Schwarzacher
T
T
Flavell
R
R
D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA; D001467:Hordeum / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D004247:DNA