Chromatographic separation and photoelectrochemical detection of sodium nitroprusside and its degradative and metabolic products.
Ion-pair chromatography has been used for the separation of nitroprusside ion and its photochemical hydrolytic and metabolic products. Organic modifier and pH were adjusted for maximum separation of the ions. Methanol was selected as the organic modifier in a pH range 5-8 and tetrabutylammonium perchlorate was used as the ion-pairing reagent. Ions were detected with a photoelectrochemical detector as described by Krull. A modification of this procedure was used to detect nitroprusside ion in spiked serum samples.
Ayers
T H
TH
Department of Medicinal Chemistry, College of Pharmacy, University of Georgia, Athens 30602.
Metwally
M E
ME
Honigberg
I L
IL
D002845:Chromatography; D004563:Electrochemistry; D005292:Ferricyanides / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D007202:Indicators and Reagents; D009599:Nitroprusside / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D010777:Photochemistry; D012997:Solvents
D005292:Ferricyanides; D007202:Indicators and Reagents; D012997:Solvents; D009599:Nitroprusside