[Methodological errors in amino acid assay in biological fluids].
Numerous methodological errors have been observed in the measurement of free-amino acids in the biological fluids. Some of them are related to the presence or the formation of amino acids or ninhydrin-positive compounds in tubes or anticoagulants used for the blood sampling. Hemolysis or blood clotting modify the concentration of some amino acids in plasma. A special error observed with the capillary blood is related to the amino acids present on area of the skin. Bacterial contamination of urines or buffers used for the analysis can also modify the results of the amino acids assays.
Parvy
P
P
Laboratoire de Biochimie Médicale B, Hôpital Necker, Enfants Malades, Paris.
Bardet
J
J
Rabier
D
D
Kamoun
P
P
D000596:Amino Acids / Q000097:blood / Q000737:chemistry* / Q000652:urine; D001681:Biological Assay / Q000379:methods*; D006801:Humans
D000596:Amino Acids
Les erreurs méthodologiques dans l'analyse des acides aminés des liquides biologiques.