Evidence for microbial polysaccharide preparations containing polyester substituents.
CPMAS 13C-n.m.r. spectroscopy was employed to characterize the composition and solid phase morphology of gellan, welan, rhamsan and NW11. Spectra indicated that commercial preparations of these polysaccharides, which share a similar molecular backbone, contain a non-carbohydrate component exhibiting four inequivalent carbon atoms. Isolation of this component, followed by 13C-n.m.r. in CHCl3 and MS analysis, revealed its structure to be poly(beta-hydroxybutyrate). Evidence is presented which suggests that this polyester may be a covalent adduct to the above polysaccharides, although this cannot be unambiguously determined at this time. Further experimentation is in progress.
Giammatteo
P J
PJ
Texaco Research Center, Beacon, New York 12508.
Stipanovic
A J
AJ
Nero
V P
VP
Robison
P D
PD
D006885:Hydroxybutyrates / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D011091:Polyesters / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D000094122:Polyhydroxybutyrates
D006885:Hydroxybutyrates; D011091:Polyesters; D011135:Polysaccharides, Bacterial; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates