[The use of Aedes albopictus C6/36 cells in the propagation and classification of arbovirus of the Togaviridae, Flaviviridae, Bunyaviridae and Rhabdoviridae families].
C6/36 Aedes albopictus cells were infected with Brazilian arbovirus from the families Togaviridae, Flaviviridae, Bunyaviridae and Rhabdoviridae. Replication was obtained with all the studied viruses and cytopathic effect was observed with some. Viral antigen was assayed in C6/36 cell cultures for antigen was assayed in C6/36 cells by an indirect immunofluorescence test using specific mouse immune ascitic fluid. Antigen production was detected in C6/36 cells infected with all the studied viruses. The author recommends the inoculation of C6/36 cell cultures for isolation of virus from the four studied families. The immunofluorescence technique is an important tool for classification and identification of virus growing in C6/36 cells.
Figueiredo
L T
LT
Departamento de Virologia do Instituto Oswaldo Cruz, Rio de Janeiro, Brasil.
D000330:Aedes / Q000166:cytology; D000818:Animals; D001103:Arboviruses / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification; D002043:Bunyaviridae / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification; D002478:Cells, Cultured; D012209:Rhabdoviridae / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification; D014035:Togaviridae / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification
Uso de células de Aedes albopictus C6/36 na propagação e classificação de arbovírus das famílias Togaviridae, Flaviviridae, Bunyaviridae e Rhabdoviridae.