Detection of potato spindle tuber viroid (PSTV) in dormant potato tubers by concatameric cDNA probe.
The 32P-labelled concatameric insert cut out from a plasmid pSPAv6.2(+), containing 6.2 copies of a full-length PSTV, was used to detect PSTV in dormant potato tubers by dot-blot hybridisation assay. The concatameric insert probe was 4 times more sensitive than the monomeric one. This allowed the detection of 0.5 pg of viroid RNA. The sensitivity makes th eoligomeric cDNA probe a useful alternative to cDNA probes.
Zekanowski
C
C
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw.
Wełnicki
M
M
Skrzeczkowski
J
J
Zagórski
W
W
D015342:DNA Probes; D015151:Immunoblotting / Q000379:methods; D009693:Nucleic Acid Hybridization; D010942:Plant Viruses / Q000235:genetics / Q000302:isolation & purification*; D010957:Plasmids; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics; D011198:Solanum tuberosum / Q000382:microbiology*; D014772:Viroids / Q000235:genetics / Q000302:isolation & purification*
D015342:DNA Probes; D012367:RNA, Viral