Biochemical characterization and purification of the neuronal sodium-dependent noradrenaline transporter.
The protein properties of the neuronal sodium-dependent noradrenaline (NA) transporter of PC12 (rat pheochromocytoma) cells and of bovine adreno-medullary cells were studied by means of binding of 3H-desipramine (3H-DMI). 3H-DMI binding was decreased by proteases, phospholipase A2, by disulfide reducing agents and by the sulfhydryl-group alkylating agent N-ethylmaleimide. The NA transporter was partially purified by anion exchange and affinity chromatography. Tritiated desmethylxylamine (3H-DMX) bound irreversibly and in a DMI-sensitive manner to two PC12 membrane proteins (32kd and 53kd) which may represent components of the NA transporter.
Bönisch
H
H
Department of Pharmacology, University of Bonn, Federal Republic of Germany.
Martiny-Baron
G
G
Blum
B
B
Michael-Hepp
J
J
D000310:Adrenal Gland Neoplasms / Q000378:metabolism; D000313:Adrenal Medulla / Q000187:drug effects / Q000378:metabolism; D000345:Affinity Labels; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D002417:Cattle; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D003891:Desipramine; D004220:Disulfides / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009588:Nitrogen Mustard Compounds / Q000494:pharmacology; D009638:Norepinephrine / Q000378:metabolism*; D050484:Norepinephrine Plasma Membrane Transport Proteins; D010673:Pheochromocytoma / Q000378:metabolism; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D051381:Rats; D012964:Sodium / Q000502:physiology*; D013439:Sulfhydryl Reagents; D013566:Sympathomimetics / Q000494:pharmacology; D027981:Symporters
D000345:Affinity Labels; D002352:Carrier Proteins; D004220:Disulfides; D009588:Nitrogen Mustard Compounds; D050484:Norepinephrine Plasma Membrane Transport Proteins; D011506:Proteins; C493226:Slc6a2 protein, rat; D013439:Sulfhydryl Reagents; D013566:Sympathomimetics; D027981:Symporters; C026131:xylamine; D012964:Sodium; D003891:Desipramine; D009638:Norepinephrine