Analysis of T lymphocytes cloned from the synovial fluid and blood of a patient with Lyme arthritis.
Cloned T lymphocytes reactive with Borrelia burgdorferi proteins were isolated from a patient with chronic Lyme arthritis. All of the T cell clones which proliferated in response to Borrelia proteins were CD3 + CD4 + CD8 - TCR alpha beta + and HLA-DR restricted. One T cell clone (GN30) exhibited HLA-DR-restricted cytotoxic activity against antigen-presenting cells pulsed with Borrelia antigen. In response to Borrelia antigen, the T cell clones produced TNF-alpha, INF-gamma, and GM-CSF. There are at least three distinct spirochetal proteins recognized by the four T cell clones analyzed. Purified Borrelia proteins triggered the HLA-DR-restricted proliferative and cytotoxic responses, as well as lymphokine secretion by two of the T cell clones. The spirochetal protein which triggered the HLA-DR-restricted proliferative and cytotoxic activities of the T cell clone (GN30) isolated from synovial fluid is the 41 kd flagellar protein.
Yssel
H
H
Department of Human Immunology, DNAX Research Institute, Palo Alto, CA.
Nakamoto
T
T
Schneider
P
P
Freitas
V
V
Collins
C
C
Webb
D
D
Mensi
N
N
Soderberg
C
C
Peltz
G
G
D000942:Antigens, Bacterial; D001170:Arthritis, Infectious / Q000097:blood / Q000276:immunology* / Q000473:pathology; D001426:Bacterial Proteins / Q000276:immunology; D015748:Borrelia burgdorferi Group / Q000276:immunology; D015704:CD4 Antigens; D002999:Clone Cells / Q000276:immunology / Q000473:pathology; D003602:Cytotoxicity, Immunologic; D006684:HLA-DR Antigens; D006801:Humans; D008193:Lyme Disease / Q000097:blood / Q000276:immunology* / Q000473:pathology; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000096:biosynthesis; D013582:Synovial Fluid / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D015704:CD4 Antigens; D006684:HLA-DR Antigens; D008222:Lymphokines