Preliminary crystallographic analysis of class 3 rat liver aldehyde dehydrogenase.
NAD-linked aldehyde dehydrogenases (A1DH) (EC 1.2.1.3) catalyze the irreversible oxidation of a wide variety of aldehydes to their respective carboxylic acids. Crystals of a class 3 AIDH (from an Escherichia coli expression system) suitable for X-ray analysis have been obtained. These crystals, which can be grown to a size of 0.8 x 0.3 x 0.2 mm, diffract to 2.5 A resolution. Analysis of the diffraction pattern indicates that the crystals belong to the monoclinic space group P21, with cell parameters a = 65.11 A, b = 170.67 A, c = 47.15 A, and beta = 110.5 degrees. Assuming one dimer per asymmetric unit, the value Vm is calculated to be 2.45 and the solvent content of the crystal is estimated to be 50%. A self-rotation function study produced significant rotation peaks (58% of the origin) on the kappa = 180 section at psi = 90 degrees and phi = 71 degrees and 341 degrees, indicating that the pseudo-dimer axis is (or is very nearly) perpendicular to the b-axis.
Rose
J P
JP
Department of Crystallography, University of Pittsburgh, Pennsylvania 15260.
Hempel
J
J
Kuo
I
I
Lindahl
R
R
Wang
B C
BC
D000444:Aldehyde Dehydrogenase / Q000648:ultrastructure*; D000818:Animals; D003461:Crystallography; D008099:Liver / Q000201:enzymology; D011487:Protein Conformation; D051381:Rats; D011994:Recombinant Proteins; D014961:X-Ray Diffraction
D011994:Recombinant Proteins; D000444:Aldehyde Dehydrogenase