title: Multicentre evaluation of an enzyme-immunoassay for cortisol determination.
abstract: The present paper describes a multicentre evaluation of a one step enzyme-immunoassay for the determination of cortisol in serum or plasma. Data from the investigation were analysed in terms of imprecision, detection limit, and correlation with other test methods. Within-run and between-run imprecisions (coefficient of variation) of Enzymun-Test Cortisol were less than 8% and 12%, respectively. The detection limit was 30 nmol/l (11 micrograms/l). With the exception of prednisolone, only low interference was found with other endogenous steroids. A good correlation between Enzymun-Test Cortisol and HPLC, LIA, FPIA and RIA was registered, although the latter two methods showed a scattering of regression lines from the different evaluators. The results show that Enzymun-Test Cortisol can be recommended as an alternative for the measurement of cortisol. As the method is calibrated against isotope dilution-mass spectrometry, results obtained with Enzymun-Test Cortisol are in agreement with the reference method.
authors.affiliation: Institut für Klinische Chemie und Laboratoriumsmedizin, Universitätsklinik der Ruhr-Universität, Bochum, Federal Republic of Germany.
authors.forename: S
authors.initials: S
authors.lastname: Tunn
authors.forename: G
authors.initials: G
authors.lastname: Pappert
authors.forename: P
authors.initials: P
authors.lastname: Willnow
authors.forename: M
authors.initials: M
authors.lastname: Krieg
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods / Q000706:statistics & numerical data; D002624:Chemistry, Clinical / Q000379:methods / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D007124:Immunoenzyme Techniques / Q000706:statistics & numerical data
chemical_list: D006854:Hydrocortisone