N-terminal halves of gramicidin S synthetase 1, and tyrocidine synthetase 1 as novel members of firefly luciferase family.
It was found, by computer-assisted homology search, that the N-terminal halves of gramicidin S synthetase 1 and tyrocidine synthetase 1 are homologous with beetle luciferases and plant 4-coumarate:CoA ligases. The comparison of the reactions catalyzed by these enzymes showed that they are involved in similar reactions; the adenylation of their substrates and the formation of thiolester. Structural and functional implication of the sequence homology and molecular evolution of these proteins are discussed.
Toh
H
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Protein Engineering Research Institute, Osaka, Japan.
D000591:Amino Acid Isomerases / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D005075:Biological Evolution; D001330:Electronic Data Processing; D008156:Luciferases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010453:Peptide Synthases / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D012689:Sequence Homology, Nucleic Acid; D013379:Substrate Specificity
D008156:Luciferases; D000591:Amino Acid Isomerases; C022343:phenylalanine racemase (ATP-hydrolyzing); D010453:Peptide Synthases; C035180:tyrocidine synthetase