title: Dietary modulation of plasma bilirubin and of hepatic microsomal lipid peroxidation in the Gunn rat.
abstract: An in vitro assay for the simultaneous measurement of lipid peroxidation (LPO) and bilirubin degradation (BRD) activities in rat liver microsomes has been developed; a good correlation between the 2 activities was observed (r = 0.78). In the Gunn rat a lipid free diet caused an increase in plasma bilirubin (62.4 +/- 25.8%, n = 6) and a concomitant decrease in both hepatic microsomal LPO and BRD to zero. In contrast, on a 25% lipid diet there was a decrease in plasma bilirubin (46.1 +/- 3.6%; n = 8) associated with an increase in LPO (1.26 +/- 0.11 nmol/min/mg protein, and BRD (0.21 +/- 0.6 nmol/min/mg protein). Therefore, in the absence of bilirubin glucuronidation, dietary modulation of plasma bilirubin and lipid peroxidation appear to be closely associated.
authors.affiliation: Dept of Medicine, Royal Free Hospital School of Medicine, London, U.K.
authors.forename: M
authors.initials: M
authors.lastname: Joshi
authors.forename: B H
authors.initials: BH
authors.lastname: Billing
authors.forename: T
authors.initials: T
authors.lastname: Hallinan
mesh_terms: D000818:Animals; D001663:Bilirubin / Q000097:blood* / Q000378:metabolism; D004041:Dietary Fats / Q000008:administration & dosage*; D066298:In Vitro Techniques; D015227:Lipid Peroxidation; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D051381:Rats; D011911:Rats, Gunn
chemical_list: D004041:Dietary Fats; D001663:Bilirubin