Selection of donors significantly improves pig islet isolation yield.
The purpose of the present study was to test the influence of donor age on islet isolation yield in swines. Large White pigs of 10 months (group 1, n = 10) and 2-3 years (group 2, n = 5) were used. A modification of the automated method for human islet isolation was used for pig islet separation. After ductal injection of a collagenase solution (Seromed, type C-1000) the islets were separated through a continuous digestion process. In group 1 it was possible to obtain an average of 636,100 islets (volume = 476.1 mm3, insulin content = 101.4 Units). In group 2 the average total islet number was 799,800 representing a volume of 1190.6 mm3 (Insulin content 210 Units). Total islet number was not significantly different in the two groups, however when islet volume and total insulin content were considered, older animals allowed a significantly higher yield (P less than 0.01 for volume and P less than 0.05 for insulin content). These findings indicate that it is possible to separate large number of pig islets from both young (10 months) and older (2-3 years) animals and that selection of donor pigs will improve islet isolation yield. These results may be of assistance in the definition of requirements for more effective islet isolation in swines.
Socci
C
C
Department of Surgery, Medicine and Pathology, Scientific Institute H. San Raffaele, University of Milan, Italy.
Ricordi
C
C
Davalli
A M
AM
Staudacher
C
C
Baro
P
P
Vertova
A
A
Freschi
M
M
Gavazzi
F
F
Braghi
S
S
Pozza
G
G
D000818:Animals; D002452:Cell Count; D002469:Cell Separation; D007515:Islets of Langerhans / Q000254:growth & development; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D013552:Swine; D014019:Tissue Donors