Structural features of the amplified N-myc oncogene detected by micrococcal nuclease digestion of neuroblastoma cell nuclei.
The structure of chromatin containing amplified N-myc in neuroblastoma and retinoblastoma cells was investigated using micrococcal nuclease digestion of isolated nuclei. The size distribution of DNA fragments containing N-myc, produced by micrococcal nuclease digestion of nuclei, was determined and compared to that of DNA containing the structural gene for dihydrofolate reductase. A perturbation of the native structure of chromatin containing N-myc was evident from the association of N-myc with more extensively digested DNA when compared with chromatin containing dihydrofolate reductase.
Telford
D J
DJ
Children's Leukaemia and Cancer Research Unit, Prince of Wales Children's Hospital, Sydney, New South Wales, Australia.
Stewart
B W
BW
D002467:Cell Nucleus / Q000737:chemistry*; D015342:DNA Probes; D005134:Eye Neoplasms / Q000235:genetics / Q000648:ultrastructure; D005784:Gene Amplification / Q000235:genetics*; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D008836:Micrococcal Nuclease; D008970:Molecular Weight; D009447:Neuroblastoma / Q000235:genetics* / Q000648:ultrastructure; D012175:Retinoblastoma / Q000235:genetics / Q000648:ultrastructure; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D008836:Micrococcal Nuclease