title: Determination of methotrexate in serum: the aca MTHO immunoassay and HPLC compared.
abstract: The determination of methotrexate on the aca clinical analyser (aca MTHO method) was evaluated and the method compared with a fluorescence polarisation immunoassay (FPIA) and a specific high-performance liquid chromatography (HPLC) procedure. The within series and the between days coefficients of variation of measurements in human pool serum spiked with methotrexate (0.07 mumol/l to 15 mumol/l) ranges from 11.7% to 2.5% and 14.0% to 5.8%, respectively. The calibration was found to be stable for 9 weeks. There was a good correlation between the results of the MTHO method when compared with FPIA and HPLC. At low methotrexate concentration, the results of HPLC were on average 11% and 14% lower than those obtained by the MTHO assay and FPIA respectively.
authors.affiliation: Institut für Klinische Chemie I, Medizinische Hochschule Hannover.
authors.forename: W
authors.initials: W
authors.lastname: Martz
authors.forename: M
authors.initials: M
authors.lastname: Oellerich
authors.forename: G
authors.initials: G
authors.lastname: Schumann
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D005454:Fluorescence Polarization; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000706:statistics & numerical data; D008727:Methotrexate / Q000097:blood*; D015203:Reproducibility of Results
chemical_list: D008727:Methotrexate