title: Avidin-peroxidase: synthesis and HPLC isolation of highly sensitive oligomers.
abstract: Optimal conditions for the preparation of avidin-peroxidase conjugates by the periodate method were studied. A method based on hydrophobic interaction chromatography was developed for the isolation of active oligomers. The probable structure of oligomers with highest sensitivity is discussed.
authors.affiliation: Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
authors.forename: G V
authors.initials: GV
authors.lastname: Gololobov
authors.forename: A M
authors.initials: AM
authors.lastname: Shuster
authors.forename: I K
authors.initials: IK
authors.lastname: Zalite
authors.forename: A G
authors.initials: AG
authors.lastname: Gabibov
authors.forename: A G
authors.initials: AG
authors.lastname: Rabinkov
mesh_terms: D001360:Avidin / Q000737:chemistry*; D001710:Biotin; D002851:Chromatography, High Pressure Liquid; D006735:Horseradish Peroxidase / Q000138:chemical synthesis / Q000737:chemistry*; D010084:Oxidation-Reduction; D011108:Polymers / Q000138:chemical synthesis / Q000302:isolation & purification
chemical_list: D011108:Polymers; D001360:Avidin; D001710:Biotin; D006735:Horseradish Peroxidase