Multinucleation of macrophages with water-solubilized lignin derivatives in vitro.
When macrophages derived from bone marrow were incubated with lignin derivative at 10 micrograms/ml, they formed multinucleated giant cells containing more than sixty nuclei. Cine-microscopic observation revealed that the formation of the giant cells was due to cell fusion. This phenomenon was specific for primary-cultured macrophages, and was not observed with either a macrophage cell line or established cell lines derived from other tissues.
Sorimachi
K
K
Department of Microbiology, Dokkyo University School of Medicine, Tochigi, Japan.
Akimoto
K
K
Yamazaki
S
S
Niwa
A
A
Toda
S
S
Yasumura
Y
Y
D000818:Animals; D001853:Bone Marrow / Q000648:ultrastructure; D001854:Bone Marrow Cells; D002459:Cell Fusion / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects* / Q000648:ultrastructure; D002478:Cells, Cultured; D015726:Giant Cells / Q000187:drug effects* / Q000648:ultrastructure; D008031:Lignin / Q000494:pharmacology*; D051381:Rats
D008031:Lignin