title: Ostertagia ostertagi: isolation and partial characterization of somatic and metabolic antigens.
abstract: One metabolic (ES) and two somatic extracts (AS and MS) were prepared from Ostertagia ostertagi. Partial characterization of the three preparations was accomplished by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblot techniques. In immunoblots, AS showed a low number of bands that reacted weakly with sera collected from infected calves. MS reacted strongly with the homologous sera, and a sharp group of bands appeared from 12 to 14 kDa, increasing in intensity as the infection progressed. ES showed a group of strongly immunogenic bands in the range of 16-22 kDa. The three preparations were also tested for reactivity with specific anti-Ostertagia antibodies in an enzyme-linked immunosorbant assay (ELISA). The ELISA results showed that all three worm extracts contained antigen epitopes recognized by circulating antibody in sera taken from O. ostertagi-infected calves. The strongest response was seen when antibodies of the IgG1 isotype were reacted with MS and ES. When sera taken from O. radiatum-infected calves were used both somatic extracts showed high levels of cross-reactivity.
authors.affiliation: Instituto Nacional de Investigaciones Agrarias (I.N.I.A.), Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Canals
authors.forename: L C
authors.initials: LC
authors.lastname: Gasbarre
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D000947:Antigens, Helminth / Q000276:immunology / Q000302:isolation & purification*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D008297:Male; D010028:Ostertagia / Q000276:immunology*; D010029:Ostertagiasis / Q000276:immunology / Q000662:veterinary
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth