Expression of ras-like proteins in embryonic and adult cells of Xenopus laevis.
A polyclonal antibody raised against v-Ha-ras p21 was purified and its specificity was checked on Ha-ras transformed cell lines. It was used to immunoprecipitate p21 from different Xenopus laevis cell types: brain cells, blood cells, and embryonic material. By one-dimensional Western blot analysis, we show that ras p21 is synthesized very early in oogenesis and accumulates throughout vitellogenesis. The ras p21 content, estimated to be 1.1 ng in the full-grown oocyte, remains constant during oocyte maturation and egg cleavage. Increase in the amount of ras p21 occurs at the beginning of neurulation. Two-dimensional Western blot patterns reveal the presence of multiple molecular forms of p21 in all Xenopus cell types studied. The numerous resolved polypeptides were ascribed to the expression of at least two different ras genes. Furthermore, specific charge modifications of the ras polypeptides are observed in brain, blood, and embryonic cells. During oogenesis and early embryonic development, differences in two-dimensional patterns mainly concern variations in the relative amounts of the different polypeptides. The results are discussed in relation to the well documented synthesis activities of the growing oocyte and of the early developing embryo.
Hanocq-Quertier
J
J
Laboratoire de Biologie du Développement, Univesité libre de Bruxelles, Belgium.
Hanocq
F
F
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D015153:Blotting, Western; D001921:Brain / Q000196:embryology / Q000378:metabolism; D002472:Cell Transformation, Viral; D015180:Electrophoresis, Gel, Two-Dimensional; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D015689:Oncogene Protein p21(ras) / Q000096:biosynthesis* / Q000276:immunology; D009866:Oogenesis; D011233:Precipitin Tests; D051381:Rats; D014407:Tumor Cells, Cultured; D014818:Vitellogenesis; D014982:Xenopus laevis
D000906:Antibodies; D015689:Oncogene Protein p21(ras)