Early induction of ornithine decarboxylase occurs simultaneously with inositol phosphate accumulation in concanavalin A-stimulated rat thymocytes.
Stimulation of rat thymocytes with the lectin ConA produced an early peak of ornithine decarboxylase (ODC) activity within 10 min. This ODC induction appeared as early as the well-known inositol phosphate accumulation following mitogenic stimulation, and may be part of the signal transduction mechanism. The distribution of counts among the inositol phosphates was constant during the overall time of Concanavalin A (ConA) stimulation. We conclude that early induction of pre-existing ODC may be independent of protein kinase C action.
Valette
L
L
Institute National de la Santé et de la Recherche Médicale U205, Laboratoire de Chimie Biologique, Villeurbanne, France.
Fonlupt
P
P
Prigent
A F
AF
Macovschi
O
O
Lagarde
M
M
D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D004789:Enzyme Activation; D004790:Enzyme Induction; D007295:Inositol Phosphates / Q000378:metabolism*; D008213:Lymphocyte Activation; D008297:Male; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*
D007295:Inositol Phosphates; D003208:Concanavalin A; D011493:Protein Kinase C; D009955:Ornithine Decarboxylase