Effects of free fatty acids on the binding of steroid hormones to bovine serum albumin.
The effects of binding of free fatty acids (FFA) to bovine serum albumin (BSA) on steroid hormone binding to BSA were examined. The FFA studied included myristic, palmitic, stearic, oleic and linoleic acids. The binding coefficient K was estimated from the changes in the equilibrium partition coefficient between the aqueous and the hexane phase caused by the addition to BSA to the aqueous phase. A noticeable effect of FFA binding (molar ratio FFA/BSA, 2:1) on the affinities of alpha-estradiol, ethynylestradiol and dehydroisoandrosterone to BSA was not observed: however, the affinities of progesterone, androsterone and testosterone were distinctly enhanced by FFA binding. Furthermore, the elution profiles of gel filtration chromatography clearly showed that progesterone and testosterone are easily liberated from the hormone/BSA complexes and that stronger binding of these hormones to BSA is caused by binding of FFA to BSA. The affinity of ethynylestradiol to BSA is stronger than that of progesterone and testosterone and is not affected by palmitic acid binding to BSA.
Watanabe
S
S
Kanagawa Prefectural Public Health Laboratory, Yokohama, Japan.
Tani
T
T
Watanabe
S
S
Seno
M
M
D000728:Androgens / Q000378:metabolism*; D000738:Androsterone / Q000378:metabolism; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D004967:Estrogens / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology*; D006586:Hexanes; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000494:pharmacology; D011374:Progesterone / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D012996:Solutions; D013739:Testosterone / Q000378:metabolism; D014867:Water
D000728:Androgens; D004967:Estrogens; D005230:Fatty Acids, Nonesterified; D006586:Hexanes; D010169:Palmitic Acids; D012996:Solutions; D014867:Water; D012710:Serum Albumin, Bovine; D019308:Palmitic Acid; D013739:Testosterone; D011374:Progesterone; D000738:Androsterone