[Use of monoclonal antibodies for the antigenic analysis of West Nile viral strains isolated in Madagascar. Contribution to the understanding of the epidemiological cycle].
The antigenic relationship of 53 MADAGASCAR West Nile isolates to each other and to the two prototype viruses (Eg 101 and G 2266) was assessed using monoclonal antibodies. In MADAGASCAR exist 5 antigenic groups: 4 which are much closer to the Egyptian strain Eg 101 than to the Indian, and antigenically distinct from South african H 442 strain. One other is closely related to Indian strain G 2266. Antigenic variations are observed in every periods of transmission cycle. Some differences between strains isolated in a same region are also observed. MADAGASCAR is an exchange place for West Nile virus through the instrumentality of migratory birds.
Morvan
J
J
Unité de Recherche sur les Arbovirus, Institut Pasteur, Antananarivo, Madagascar.
Fontenille
D
D
Besselaar
T G
TG
Coulanges
P
P
D000330:Aedes; D000818:Animals; D000911:Antibodies, Monoclonal; D000940:Antigenic Variation / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology; D001717:Birds; D002519:Ceratopogonidae; D004199:Disease Vectors; D005455:Fluorescent Antibody Technique; D006801:Humans; D007303:Insect Vectors; D008270:Madagascar / Q000453:epidemiology; D010311:Parrots; D012703:Serotyping; D014901:West Nile Fever / Q000453:epidemiology / Q000276:immunology* / Q000635:transmission; D014902:West Nile virus / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral
Utilisation des anticorps monoclonaux pour l'analyse antigénique des souches de virus West Nile isolées à Madagascar. Apport pour la compréhension du cycle épidémiologique.