The development of a radioimmunoassay system for testosterone (T) and dihydrotestosterone (DHT). Part 1. The preparation of the T-derivatives and T-protein conjugates.
Testosterone-17-hemisuceinate (T-17-HS) by esterification with succinic anhydride technique and Testosterone-3-carboxy-methyl-oxyme (T-3-CMO) derivatives were synthesized. The T-protein (BSA) conjugates were prepared by the condensation with carbodiimide technique, for the T-17-HS derivative and by the mixed anhydride technique, for the T-3-CMO. Both the derivatives and the T-BSA conjugates were characterized by melting point, ultraviolet and infrared spectra, thin layer chromatography. The physico-chemical characteristics and the hapten-protein incorporation being convenient: 28 mol T/mol BSA for T-17-HS-BSA and 38 mol T/mol BSA for T-3-CMO-BSA, the T-carrier conjugates are adequate for their use as immunogens.
Neacşu
E
E
C. I. Parhon Institute of Endocrinology, Bucharest, Romania.
Oniciu
D
D
Simionescu
L
L
D000818:Animals; D002855:Chromatography, Thin Layer; D013196:Dihydrotestosterone / Q000737:chemistry*; D006801:Humans; D011863:Radioimmunoassay / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D013739:Testosterone / Q000031:analogs & derivatives* / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry*
C067733:testosterone-3-carboxymethyloxime; D013196:Dihydrotestosterone; D013739:Testosterone; C029763:testosterone-17-succinate