title: [Evaluation of specificity of EIA kit (ED-007)].
abstract: EIA (Eitest: Eisai) and Gelatin Particle Agglutination: PA (SERODIA: Fujirebio) assays were performed for the detection of HTLV-I antibodies with 10,780 sera from patients since 1986. Eleven point five percent were reactive by both EIA and PA, while 1.1% was PA(+), EIA(-), these PA false positive occurred on low titer. Conversely 0.2% on EIA positive seems PA false negative because of Western blot (WB) positivity. Zero point nine percent was EIA(+), PA(-), of these 80.6% were not inhibited by EIA confirmatory test. The main cause of EIA false positive was due to reactivity of auto antibody with MT-2 cell lysate. We used new EIA (ED-007: Eisai) coated with HTLV-I antigen purified from supernatant of culture medium of MT-2 cell. The results completely matched with EIA confirmatory test and WB. Cut off index value of sera from SLE patients were down to 0.2 (mean +/- SD) from 0.7 +/- 0.4 of Eitest ATL. EIA (ED-007) are probably useful and more specific assays for the detection of HTLV-I antibodies, especially, the sera of patients with autoimmune diseases.
authors.affiliation: Blood Transfusion Service, Kumamoto University Hospital.
authors.forename: Y
authors.initials: Y
authors.lastname: Nishimura
authors.forename: N
authors.initials: N
authors.lastname: Fukuoka
authors.forename: Y
authors.initials: Y
authors.lastname: Fukuyoshi
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuo
authors.forename: K
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: K
authors.initials: K
authors.lastname: Takatsuki
mesh_terms: D000372:Agglutination Tests; D005069:Evaluation Studies as Topic; D005780:Gelatin; D015481:HTLV-I Antibodies / Q000032:analysis*; D015490:HTLV-I Infections / Q000175:diagnosis; D006801:Humans; D007124:Immunoenzyme Techniques; D011933:Reagent Kits, Diagnostic / Q000592:standards*
chemical_list: D015481:HTLV-I Antibodies; D011933:Reagent Kits, Diagnostic; D005780:Gelatin