The influence of CS-3000 plateletapheresis on blood donors and on platelet concentrates.
Platelet concentrates were prepared from blood of donors by means of Fenwal CS-3000, or by double centrifugation method. Platelets from CS-3000 were cryopreserved with 5% DMSO for 2-4 weeks. After thawing and washing platelets were stored in room temperature for 6 hours. Immediately after plateletapheresis the platelet count decreased by 24% and leukocyte count, Ht and Hb diminished on average 30%, 22% and 18% respectively. The efficiency of separation procedure was 45% and white cells contamination 5.8 +/-8.1 x 10(11). Significant morphological changes and the decrease in total ATP comparing to double centrifugation method were observed. Storage of platelets for 6 hours in room temperature didn't cause their quality changes. Single donor plateletapheresis method can prevent alloimmunization and diminish the risk of transmission of the viruses.
Kotelba-Witkowska
B
B
Instytut Hematologii, Warszawa, Poland.
Mrowiec
Z R
ZR
Daszyński
J
J
Skrzeczkowska
E
E
Stachecki
B
B
Kaszkowiak
M
M
Jakuboszczak
E
E
D001782:Blood Donors; D001792:Blood Platelets / Q000502:physiology*; D015925:Cryopreservation; D004121:Dimethyl Sulfoxide; D006801:Humans; D010976:Platelet Count; D010983:Plateletpheresis / Q000379:methods*
D004121:Dimethyl Sulfoxide