title: Renal preservation after warm ischemia using oxygen free radical scavengers to prevent reperfusion injury.
abstract: Oxygen free radicals damage kidneys and accumulate during the period of preservation prior to transplantation. We hypothesized that a perfusate containing either an oxygen free radical scavenger such as ceruloplasmin, or an iron-chelating agent such as deferoxamine, would improve kidney preservation. Thirty-eight mongrel dogs underwent autotransplantation of the left kidney after 30 min of warm ischemia and 48 hr of machine perfusion (MOX-100, Water Instruments, Rochester, MN) at 5 degrees C and pH of 7.4. The right kidney was removed at the time of autotransplantation. Four blind code-labeled preservation solutions were tested. SGF-I was used for the control group (Group 1, n = 13), and the remaining animals were transplanted with kidneys preserved with one of three solutions modified from the basic SGF-I solution: Group 2, SGF-I plus deferoxamine (656 mg/liter), n = 8; Group 3, SGF-I ceruloplasmin enriched (72 mg/dl), n = 8; and Group 4, SGF-I ceruloplasmin reduced (3.4 mg/dl), n = 9. Serum creatinine levels were measured daily for 2 weeks and survival curves for each of the four groups were estimated by the Kaplan-Meier method. Peak mean serum creatinine levels +/- standard errors in Groups 1 through 4 were 12.6 +/- 1.97, 7.8 +/- 0.90, 7.1 +/- 1.26, and 8.2 +/- 1.09, respectively. Repeated measures analysis of variance showed statistically significant differences between the groups with respect to their serum creatinine profiles (Wald's test x2 with 3 df = 22.39, P value less than 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Surgery, University of Minnesota, Minneapolis 55455.
authors.forename: P
authors.initials: P
authors.lastname: Baron
authors.forename: O
authors.initials: O
authors.lastname: Gomez-Marin
authors.forename: C
authors.initials: C
authors.lastname: Casas
authors.forename: J
authors.initials: J
authors.lastname: Heil
authors.forename: N
authors.initials: N
authors.lastname: Will
authors.forename: R
authors.initials: R
authors.lastname: Condie
authors.forename: B
authors.initials: B
authors.lastname: Burke
authors.forename: J S
authors.initials: JS
authors.lastname: Najarian
authors.forename: D E
authors.initials: DE
authors.lastname: Sutherland
mesh_terms: D000818:Animals; D002570:Ceruloplasmin / Q000494:pharmacology; D003404:Creatinine / Q000097:blood; D003676:Deferoxamine / Q000494:pharmacology; D004285:Dogs; D016166:Free Radical Scavengers; D006358:Hot Temperature; D007511:Ischemia / Q000503:physiopathology*; D007668:Kidney; D009926:Organ Preservation / Q000379:methods*; D010100:Oxygen / Q000378:metabolism*; D012016:Reference Values; D012079:Renal Circulation; D015427:Reperfusion Injury / Q000401:mortality / Q000517:prevention & control*; D016019:Survival Analysis
chemical_list: D016166:Free Radical Scavengers; D003404:Creatinine; D002570:Ceruloplasmin; D003676:Deferoxamine; D010100:Oxygen