The effects of scleroderma sera on endothelial cell survival in vitro.
The effects of sera and of platelet-poor plasma from patients with scleroderma on endothelial cell survival in vitro were studied. The survival ratio of rat heart endothelial cells was studied both in 10% test serum and in 10% platelet-poor plasma. Sera from patients with scleroderma decreased the survival ratio significantly when compared with sera from normal controls. In contrast, there was no significant difference between platelet-poor plasma from patients with scleroderma and that from normal controls. Our data indicate that platelets in the patients with scleroderma may cause vascular damage by affecting endothelial cell survival.
Etoh
T
T
Department of Dermatology, Faculty of Medicine, University of Tokyo, Japan.
Igarashi
A
A
Iozumi
K
K
Ishibashi
Y
Y
Takehara
K
K
D000328:Adult; D000368:Aged; D000818:Animals; D000974:Antibodies, Antinuclear / Q000032:analysis; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D002503:Centromere / Q000276:immunology; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D008875:Middle Aged; D009500:Neutralization Tests; D051381:Rats; D012595:Scleroderma, Systemic / Q000097:blood*; D016212:Transforming Growth Factor beta / Q000276:immunology
D000974:Antibodies, Antinuclear; D016212:Transforming Growth Factor beta