title: Long term production of SP1 by amniotic cells.
abstract: We verified the long term production of SP1 by amniotic cells. After 20 days of incubation, protein synthesis was inhibited by adding puromycin to the culture medium and stimulated by the addition of foetal calf serum to the culture media.
authors.affiliation: Department of Obstetrics and Gynecology, University of Pavia, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Guaschino
authors.forename: C
authors.initials: C
authors.lastname: Aprile
authors.forename: L
authors.initials: L
authors.lastname: Bacchella
authors.forename: M L
authors.initials: ML
authors.lastname: Masanti
authors.forename: A
authors.initials: A
authors.lastname: Spinillo
mesh_terms: D000650:Amnion / Q000166:cytology*; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007231:Infant, Newborn; D011247:Pregnancy; D011268:Pregnancy-Specific beta 1-Glycoproteins / Q000096:biosynthesis*; D011863:Radioimmunoassay
chemical_list: D011268:Pregnancy-Specific beta 1-Glycoproteins