title: An optimized procedure for the separation of amino acid phenylthiohydantoins by reversed-phase HPLC.
abstract: An optimization procedure for the separation of 24 PTH-amino acids by high-performance liquid chromatography on an inexpensive Merck Superspher Si 60 RP-8, (4.0 x 250 mm) column with PTH-Nle as an internal standard is described. The effects of pH, ionic strength, temperature and gradient were investigated. Using conventional HPLC equipment, the practical detection limit is about 5 pmol.
authors.affiliation: Department of Biochemistry, University of Uppsala, Sweden.
authors.forename: B
authors.initials: B
authors.lastname: Persson
authors.forename: D
authors.initials: D
authors.lastname: Eaker
mesh_terms: D000596:Amino Acids / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D010669:Phenylthiohydantoin / Q000031:analogs & derivatives* / Q000302:isolation & purification; D015203:Reproducibility of Results; D013696:Temperature
chemical_list: D000596:Amino Acids; D010669:Phenylthiohydantoin