[Purification of retinal S-antigen and its uveiticogenic activity].
Retinal S-antigen, a soluble protein present in the photosensory layer of the retina, has been shown to be involved in the pathogenesis of some uveitis. The procedure for the isolation of S-antigen has been continually improved and perfected. In light of the method of A1-Mahdawi and coworkers, with some modification, we obtained good yields of highly purified S-antigen (0.325 mg per retina) from bovine retinas. Experimental auto-immune uveitis (EAU) was successfully induced by injecting the S-antigen into the hind footpad of guinea pigs. The EAU was characterized by lymphocyte infiltration in the pars plana and vitreous base, a counterpart of human intermediate uveitis.
Yang
P Z
PZ
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou.
D000818:Animals; D000941:Antigens / Q000302:isolation & purification*; D019393:Arrestin; D001324:Autoantigens / Q000302:isolation & purification*; D002417:Cattle; D002924:Ciliary Body / Q000473:pathology; D005136:Eye Proteins / Q000302:isolation & purification*; D005260:Female; D006168:Guinea Pigs; D012160:Retina / Q000276:immunology*; D014605:Uveitis / Q000209:etiology / Q000276:immunology*; D014822:Vitreous Body / Q000473:pathology
D000941:Antigens; D019393:Arrestin; D001324:Autoantigens; D005136:Eye Proteins