title: [Storage of granulocytes].
abstract: Granulocytes transfusions set numerous problems and are less and less used. The very short maintenance of the granulocyte functions (chemotaxis) limits their use are a clinical tool (e.g. for localizing infections and for transfusions). Neither granulocyte collection by centrifugation methods (continuous or discontinuous flow) nor glucocorticoid premedication significantly alter granulocytes functions (in particular chemotaxis). Liquid preservation methods give better results than cryogenic methods. Optimal storage parameters are a temperature of 22 degrees C and a pH level close to 7.4. Chemotaxis is the most sensitive indicator of granulocyte damages in connection with storage. Bicarbonate buffer, glucose, proteins and reducing agents supplementation significantly improve chemotaxis maintenance. Cryogenic methods are less studied and less used. They allow storage of only little amounts of granulocytes. In conclusion, efficient granulocyte storage (over several days) would allow an easier use of these cells in many fields, but unfortunately, no solution is yet available.
authors.affiliation: Centre régional de transfusion sanguine de Nancy-Brabois, Vandoeuvre-les-Nancy.
authors.forename: L
authors.initials: L
authors.lastname: Verstraeten
authors.forename: M
authors.initials: M
authors.lastname: Marchand-Arvier
authors.forename: F
authors.initials: F
authors.lastname: Schooneman
authors.forename: C
authors.initials: C
authors.lastname: Vigneron
mesh_terms: D002634:Chemotaxis, Leukocyte / Q000502:physiology; D006098:Granulocytes / Q000502:physiology / Q000637:transplantation; D006801:Humans; D011309:Preservation, Biological / Q000379:methods*
vernacular_title: Conservation des polynucléaires neutrophiles.