title: Evidence that the protein components of bovine erythrocyte green heme binding protein and flavin reductase are identical.
abstract: Bovine erythrocyte green heme binding protein and bovine erythrocyte flavin reductase have been isolated in highly purified forms and subjected to amino acid analysis and N-terminal amino acid sequence analysis. The two proteins possess similar amino acid compositions and identical N-terminal amino acid sequences. Moreover, the two proteins are immunochemically cross-reactive and are indistinguishable when compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and by double diffusion technique. This study provides evidence that the protein components of bovine erythrocyte green heme binding protein and flavin reductase are identical.
authors.affiliation: Department of Biological Chemistry, Medical School, University of Michigan, Ann Arbor 48109-0606.
authors.forename: K S
authors.initials: KS
authors.lastname: Quandt
authors.forename: F
authors.initials: F
authors.lastname: Xu
authors.forename: P
authors.initials: P
authors.lastname: Chen
authors.forename: D E
authors.initials: DE
authors.lastname: Hultquist
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D042966:Cytochrome-B(5) Reductase / Q000097:blood* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism*; D007106:Immune Sera; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid
chemical_list: D007106:Immune Sera; D010446:Peptide Fragments; D042966:Cytochrome-B(5) Reductase