title: Comparison of measurements of human sperm motility characteristics by the automated CellSoft system and time-exposure photomicrography.
abstract: Human sperm motility characteristics in 28 semen samples with sperm concentrations less than 40 x 10(6) ml-1, as determined by the World Health Organization manual analysis (WHO, 1987), were measured by the automated CellSoft semen analyser (Cryo Resources Ltd, New York, NY, USA) using different system parameter settings (Mortimer & Mortimer, 1988a). The results were compared with those obtained by time-exposure photomicrographic (TEP) analysis. It was found that the settings of the minimum video frame rate and the threshold velocity used to distinguish motile from immotile sperm by the automated CellSoft system had a significant influence on measurements of percentage motility but not on linear velocity. At the five different parameter settings used in the present study, the automated CellSoft system gave significantly lower mean values for percentage motility in comparison with the WHO manual and TEP analyses. Measurements for linear velocity between the automated CellSoft system and TEP analyses were found not to be significantly different in these defined semen samples.
authors.affiliation: Department of Obstetrics and Gynaecology, University of Hong Kong, Queen Mary Hospital.
authors.forename: S Y
authors.initials: SY
authors.lastname: Chan
authors.forename: G H
authors.initials: GH
authors.lastname: Zhang
authors.forename: T
authors.initials: T
authors.lastname: Lo
authors.forename: A
authors.initials: A
authors.lastname: Leung
authors.forename: C
authors.initials: C
authors.lastname: Wang
mesh_terms: D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D010784:Photomicrography; D013076:Sperm Count; D013081:Sperm Motility