An enzyme immunoassay for atractyloside, the nephrotoxin of Callilepis laureola (Impila).
Tubers of Callilepis laureola, a traditional remedy, contain an inhibitor of oxidative phosphorylation; atractyloside. A "competitive" ELISA was developed, using the antiserum produced to an atractyloside-protein conjugate. An ovalbumin-atractyloside conjugate was adsorbed to microtitre wells and plates incubated with sample (atractyloside or tuber extract) and antiserum. After successive incubation with secondary antibody-enzyme conjugate and substrate, the absorbance was read at 405 nm. Antibody working dilution was low, but results, confirmed by thin layer chromatography, indicate the immunoassay has diagnostic potential.
Bye
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Department of Biochemistry, University of Natal, Pietermartizburg, Republic of South Africa.
Coetzer
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Dutton
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D000818:Animals; D000917:Antibody Formation; D001278:Atractyloside / Q000032:analysis*; D002855:Chromatography, Thin Layer; D004797:Enzyme-Linked Immunosorbent Assay; D010946:Plants, Medicinal / Q000032:analysis*; D011817:Rabbits / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology
D001278:Atractyloside; D012710:Serum Albumin, Bovine