title: Procainamide inhibits DNA methyltransferase in a human T cell line.
abstract: Procainamide, a widely used antiarrythmic, causes DNA hypomethylation in the human T cell line Jurkat, but the mechanism is unknown. We report that procainamide inhibits the DNA methyltransferase catalyzed transfer of methyl groups from S-adenosylmethionine to DNA, but has no effect on other known regulators of DNA methylation. Our results suggest that procainamide could inhibit cellular DNA methylation by inhibiting DNA methyltransferase activity.
authors.affiliation: Department of Internal Medicine, University of Michigan, Ann Arbor 48109-0531.
authors.forename: L S
authors.initials: LS
authors.lastname: Scheinbart
authors.forename: M A
authors.initials: MA
authors.lastname: Johnson
authors.forename: L A
authors.initials: LA
authors.lastname: Gross
authors.forename: S R
authors.initials: SR
authors.lastname: Edelstein
authors.forename: B C
authors.initials: BC
authors.lastname: Richardson
mesh_terms: D000818:Animals; D002460:Cell Line; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000378:metabolism*; D008745:Methylation; D010743:Phospholipids / Q000378:metabolism; D011342:Procainamide / Q000494:pharmacology*; D051381:Rats; D012435:S-Adenosylhomocysteine / Q000378:metabolism*; D012436:S-Adenosylmethionine / Q000378:metabolism*; D013601:T-Lymphocytes / Q000201:enzymology*
chemical_list: D010743:Phospholipids; D012436:S-Adenosylmethionine; D012435:S-Adenosylhomocysteine; D004248:DNA (Cytosine-5-)-Methyltransferases; D011342:Procainamide