[Application of anti-p-105 monoclonal antibody to analysis of mode of action of antitumor agents].
For the elucidation of the mode of action of anti-tumor agents, the cell cycle analysis of etoposide and podophyllotoxin was examined by means of the multiparameter flow cytometry using an anti-p-105 monoclonal antibody. The p-105 is one of the proliferation associated nuclear antigens and its expression increases with a cell cycle progression, especially in mitotic phase. Thus, we have applied its anti-monoclonal antibody to the discrimination between M and G2 phases of the gastric cancer cells. The results revealed that etoposide caused a G2 block and retarded an S phase transition, and podophyllotoxin caused both G2 and M phase blocks in the gastric cancer cells. This cell cycle analysis using an anti-p-105 monoclonal antibody would be a useful analysis of the antitumor agents, especially for M phase analysis of human cancer cells because of its rapidity and convenience.
Ohyama
S
S
Second Dept. of Surgery, School of Medicine, Kanazawa University, Japan.
Yonemura
Y
Y
Tugawa
K
K
Kimura
H
H
Kosaka
T
T
Miyazaki
I
I
Endo
Y
Y
Tanaka
M
M
Sasaki
T
T
D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D005047:Etoposide / Q000494:pharmacology; D006801:Humans; D008938:Mitosis; D011034:Podophyllotoxin / Q000494:pharmacology; D013274:Stomach Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; D005047:Etoposide; D011034:Podophyllotoxin