Changes of gene expression during long term adaptation of human EUE cells to a hypertonic medium: electrophoretic protein patterns and DNase I digestion in situ.
The effects of long term (1 to 10 days) growth in a hypertonic medium have been studied in human EUE cells. Following polyacrylamide gel electrophoresis, a change in the protein pattern has been found, with the progressive enhancement, during adaptation, of a 33 kDa band. Experiments of DNA digestion in situ by DNase I showed that chromatin DNA of cells grown in a hypertonic medium is more available to the enzyme cleavage. These findings show that long term hypertonic stress is able to induce a change in gene expression in EUE cells.
Manfredi Romanini
M G
MG
Dipartimento di Biologia Animale and Centro di Studio per l'Istochimica del C.N.R., Pavia, Italy.
Fraschini
A
A
Fuhrman Conti
A M
AM
Gasperi
G
G
Giuliani
A
A
Pellicciari
C
C
D002478:Cells, Cultured; D003850:Deoxyribonuclease I; D004591:Electrophoresis, Polyacrylamide Gel; D004622:Embryo, Mammalian; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D006982:Hypertonic Solutions; D008970:Molecular Weight; D011506:Proteins / Q000235:genetics* / Q000302:isolation & purification
D006982:Hypertonic Solutions; D011506:Proteins; D003850:Deoxyribonuclease I