[An improved method for the microencapsulation of liver microsomes for use in extracorporeal detoxication].
Rat liver microsomes were microencapsulated in a pure aqueous medium by means of a new technique. The wall of the microcapsules consists of a semipermeable simplex membrane which is stabilized mainly by electrostatic interactions between a polymeric polyanion (sodium cellulose sulphate) and a polymeric polycation (polydimethyldiallylammonium chloride). The metabolic as well as the mechanic parameters of the microcapsules could be markedly improved by separating the metabolic (liver microsomes) from the membrane component (sodium cellulose sulphate) in such a way that two distinct compartments are formed during the preparation of the microcapsules.
Stange
J
J
Klinik für Innere Medizin, Institut für Pathologische Biochemie.
Dummler
W
W
Brügmann
E
E
Falkenhagen
D
D
Siegmund
E
E
Ernst
B
B
Dautzenberg
H
H
D000818:Animals; D004339:Drug Compounding / Q000379:methods*; D008658:Inactivation, Metabolic / Q000502:physiology*; D008297:Male; D008862:Microsomes, Liver; D051381:Rats; D011919:Rats, Inbred Strains
Ein verbessertes Verfahren für die Mikroverkapselung von Lebermikrosomen zur Anwendung in der extrakorporalen Detoxikation.