title: Involvement of intracellular iron in the toxicity of oxidized low density lipoprotein to cultured endothelial cells.
abstract: We evaluated the role of iron in the toxicity of oxidized low density lipoprotein (Ox-LDL) to cultured vascular endothelial cells. Exposure of the endothelial cells to Ox-LDL led to cell lysis as judged by the release of lactate dehydrogenase into the medium. The presence of deferoxamine, an iron chelator, in the reaction medium containing Ox-LDL prevented the lysis of cells by Ox-LDL. Pretreatment of the cells with deferoxamine also reduced their susceptibility to the cytotoxicity of Ox-LDL. The formation of thiobarbituric acid-reacting substances (TBARS) was observed in the cells exposed to Ox-LDL. Pretreatment of cells with deferoxamine reduced the formation of TBARS which was induced by Ox-LDL. These observations suggest that the toxicity of Ox-LDL to cultured endothelial cells involves the lipid peroxidation of cellular membrane catalyzed by iron derived from the target (endothelial) cells.
authors.affiliation: Department of Geriatrics, Nagoya University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kuzuya
authors.forename: M
authors.initials: M
authors.lastname: Naito
authors.forename: K
authors.initials: K
authors.lastname: Yamada
authors.forename: C
authors.initials: C
authors.lastname: Funaki
authors.forename: T
authors.initials: T
authors.lastname: Hayashi
authors.forename: K
authors.initials: K
authors.lastname: Asai
authors.forename: F
authors.initials: F
authors.lastname: Kuzuya
mesh_terms: D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D007501:Iron / Q000502:physiology*; D015227:Lipid Peroxidation / Q000502:physiology*; D008077:Lipoproteins, LDL / Q000502:physiology*; D008563:Membrane Lipids / Q000378:metabolism*; D010084:Oxidation-Reduction; D013858:Thiobarbiturates
chemical_list: D008077:Lipoproteins, LDL; D008563:Membrane Lipids; D013858:Thiobarbiturates; D007501:Iron; C029684:thiobarbituric acid