Anti-cholera toxin IgA-, IgG- and IgM-secreting cells in various rat lymphoid tissues after repeated intestinal or parenteral immunizations.
Single antibody-secreting spot-forming cells (SFC) of the 3 main isotypes were counted in lymphoid cells from the gut lamina propria (LP), Peyer's patches (PP), mesenteric nodes (MN) and spleen (SP) of rats immunized 2-6 times intraduodenally (ID) or intraperitoneally (IP) with cholera toxin (CT). Responses for all isotypes peaked in all tissues after 4 ID- or IP-immunizations at much larger values than previously reported, and significantly decreased thereafter, except in LP and PP after IP-injections, where IgA- or IgG-SFC, but not IgM-SFC, only appeared or increased after 6 IP-doses. The highest IgA-SFC numbers (17% of tested cells) were in LP after 4 ID-doses. The isotype ratio was IgA greater than IgG greater than IgM in LP and PP after ID-injections, but IgG greater than IgA greater than IgM in MN and SP after both ID- and IP-routes. The isotype dispersion was much larger in LP and PP than in MN and SP. Our data show that at least 4 IP CT-doses were required to only elicit a few IgG- and almost no IgA-SFC in LP and PP, outlining the need for intestinal CT-immunizations to induce strong mucosal IgA-SFC responses. We also show good systemic responses elicited both by enteral and parenteral routes, and the small contribution of PP in total SFC, particularly after parenteral immunizations.
Solbreux
P M
PM
Catholic University of Louvain, International Institute of Cellular and Molecular Pathology, Brussels, Belgium.
Dive
C
C
Vaerman
J P
JP
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000921:Antibody-Producing Cells / Q000276:immunology*; D002452:Cell Count; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000008:administration & dosage / Q000276:immunology*; D007114:Immunization; D007115:Immunization Schedule; D007132:Immunoglobulin Isotypes / Q000276:immunology*; D007263:Infusions, Parenteral; D007274:Injections, Intraperitoneal; D007422:Intestines / Q000276:immunology*; D008221:Lymphoid Tissue / Q000276:immunology*; D008297:Male; D011817:Rabbits; D051381:Rats; D013154:Spleen / Q000276:immunology
D000911:Antibodies, Monoclonal; D007132:Immunoglobulin Isotypes; D002772:Cholera Toxin