[Purification and properties of the hexon antigen of canine adenovirus serotype CAV-1].
The main antigen and immunogen of canine adenovirus type 1 (CAV-1) has been purified to near homogeneity from cultural fluid of a CAV-1-infected primary cell culture by hydrophobic and anion-exchange chromatography. The hexon native form (trimer) was shown to be resistant against denaturation by SDS under conditions of SDS-PAGE performed without heating the samples. The monomer chain of the CAV-1 hexon was apparently identical in terms of electrophoretic mobility with that of the previously sequenced BAV-3 hexon polypeptide (103 kDA). In blot enzyme immunoassay only native trimers of CAV-1 hexon were detected by cross-specific polyclonal and monoclonal anti-hexon antibodies.
Verkhovskaia
L V
LV
Ulasov
V N
VN
Kiseleva
E K
EK
Khil'ko
S N
SN
Tikhonenko
T I
TI
D000256:Adenoviridae / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D015153:Blotting, Western; D002213:Capsid / Q000737:chemistry / Q000302:isolation & purification*; D036022:Capsid Proteins; D002852:Chromatography, Ion Exchange; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus
Ochistka i svoĭstva geksonobogo antigena adenovirusa sobak serotipa CAV-1.