title: Mathematical modelling of metabolic pathways affected by an enzyme deficiency.
abstract: The regulation of metabolic pathways of the red cell affected by an enzyme deficiency is studied on the basis of comprehensive mathematical models. The main steps of such a theoretical approach are outlined considering individual alterations in the kinetic properties of two regulatory enzymes: pyruvate kinase and glucose-6-phosphate dehydrogenase. It is demonstrated that mathematical modelling helps to relate the observed changes of cellular quantities as shortened life-span, or as resistance against oxidative stress to alterations in the metabolic regulation.
authors.affiliation: Institut für Biochemie, Bereich Medizin (Charité), Humboldt Universität Berlin.
authors.forename: H G
authors.initials: HG
authors.lastname: Holzhütter
authors.forename: R
authors.initials: R
authors.lastname: Schuster
authors.forename: D
authors.initials: D
authors.lastname: Buckwitz
authors.forename: G
authors.initials: G
authors.lastname: Jacobasch
mesh_terms: D000818:Animals; D003198:Computer Simulation; D004912:Erythrocytes / Q000201:enzymology*; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008433:Mathematics; D008954:Models, Biological; D011770:Pyruvate Kinase / Q000172:deficiency*
chemical_list: D011770:Pyruvate Kinase