[Experimental study of a model for granulocyte storage].
An efficient granulocytes preservation would allow an easier study of their structure and functions, of granulocytes functional diseases (e.g. chronic granulomatous disease) and an easier use of granulocytes as a clinical tool (e.g. for localizing infections or for transfusions). Unfortunately, granulocytes are nearly impossible to preserve, even in a short-term storage (less than 24 hours). This study proposes an experimental model which could improve in vitro granulocyte functions maintenance. Bicarbonate buffer, glucose, adenosine triphosphate, vitamins C and E, nicotinamide adenine dinucleotide, nicotinamide adenine dinucleotide phosphate, amikacin, and ampicillin supplementation significantly (p 0.05) improve maintenance of one or several granulocyte functions during storage.
Verstraeten
L
L
Centre régional de transfusion sanguine de Nancy-Brabois, Vandceuvre-les-Nancy.
Marchand-Arvier
M
M
Schooneman
F
F
Vigneron
C
C
D002634:Chemotaxis, Leukocyte / Q000502:physiology; D006098:Granulocytes / Q000502:physiology; D006801:Humans; D011309:Preservation, Biological / Q000379:methods*
Etude expérimentale d'un modèle de conservation des polynucléaires neutrophiles.