Distribution and elimination of brevetoxin PbTx-3 in rats.
After i.v. administration, [3H]PbTx-3 was rapidly cleared from the blood; less than 10% remained after 1 min. Within 30 min, radiolabel distributed to skeletal muscle (69.5%), liver (18.0%), and intestinal tract (8.0%). Over 24 hr, radiolabel decreased in muscle, remained constant in liver, and increased in the intestinal tract and feces. Elimination occurred via feces (75.1%) and urine (14.4%), with 9.0% remaining in the carcass after 6 days. This distribution and elimination profile suggested that the liver was the major organ of metabolism and that biliary excretion was an important route of elimination. Thin-layer chromatography confirmed the presence of brevetoxin metabolites in fecal extracts. Skeletal muscle does not appear to be a site of metabolism, but a storage compartment, from which toxin is slowly released prior to clearance by the liver. These studies are the first demonstration of in vivo brevetoxin metabolism in mammals.
Poli
M A
MA
Pathophysiology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21701-5011.
Templeton
C B
CB
Thompson
W L
WL
Hewetson
J F
JF
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005243:Feces / Q000737:chemistry; D008297:Male; D008387:Marine Toxins / Q000737:chemistry / Q000493:pharmacokinetics*; D009132:Muscles / Q000737:chemistry; D035964:Oxocins; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution; D014020:Tissue Extracts / Q000737:chemistry; D000095702:Polyether Toxins
D008387:Marine Toxins; D035964:Oxocins; D014020:Tissue Extracts; C041017:brevetoxin T17; D000095702:Polyether Toxins