title: Routed protein migration after protein extravasation and water leakage caused by cold injury.
abstract: The movement of extravasated endogenous protein induced by cold injury was investigated in the rat. Extravasated proteins were observed around the cold injury immediately after injury; within 3 hr they had moved into the ipsi- and contralateral hemispheres along the nerve fibres (routed protein migration). The water content in the areas where the extravasated protein was observed was increased, and NMR-CT scans (TR 2000 msec, TE 90 msec) showed high intensity patterns whose distribution and progression coincided with those of extravasated protein. NMR relaxation time, T1, showed a slight increase in the area where the extravasated protein was observed, but T2 value did not show any significant changes. In the opposite hemisphere, CBF decreased within 3 to 6 hours after injury. Local cerebral glucose utilization was reduced, but this change occurred more than 6 hours after injury. These results indicate that water leakage and protein extravasation occur simultaneously after the injury without any significant change of water state or protein conformation. Subsequently, a reduction of CBF is induced, which is followed by changes in cerebral metabolism.
authors.affiliation: Department of Neurology, Tokai University School of Medicine, Kanagawa, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Shinohara
authors.forename: H
authors.initials: H
authors.lastname: Ohsuga
authors.forename: S
authors.initials: S
authors.lastname: Ohsuga
authors.forename: S
authors.initials: S
authors.lastname: Takizawa
authors.forename: M
authors.initials: M
authors.lastname: Haida
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D001834:Body Water / Q000378:metabolism*; D001921:Brain / Q000648:ultrastructure; D001930:Brain Injuries / Q000097:blood / Q000378:metabolism*; D002199:Capillary Permeability; D002560:Cerebrovascular Circulation; D003080:Cold Temperature; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D001798:Blood Proteins