Binding and internalization of immunoglobulin G by cultured human trophoblast.
A preparation consisting of a high percentage of trophoblasts can be obtained by centrifuging a mixture of cell types from trypsinized term placental villi on a discontinuous gradient of Percoll. When cultured these cells maintain trophoblast characteristics as judged by immunocytochemical markers and beta-human chorionic gonadotrophin production. Incubation of cells with labelled human immunoglobulin G demonstrated their ability to bind and internalize this macromolecule in a time- and temperature-dependent manner.
Sooranna
S R
SR
Department of Obstetrics and Gynaecology, Charing Cross and Westminster Medical School, West London Hospital, UK.
Contractor
S F
SF
D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D007150:Immunohistochemistry; D013696:Temperature; D014327:Trophoblasts / Q000378:metabolism*
D007074:Immunoglobulin G