title: The effect of temperature on the ultrastructure of Histoplasma capsulatum during the mycelium-yeast transition.
abstract: The ultrastructural morphology of the early phases of mycelium-yeast transition in Histoplasma capsulatum after a temperature shift from 25 degrees C to only 34 degrees C is described. Under this condition of lower temperature oxidative phosphorylation is not completely uncoupled and maximum production of heat shock proteins (hsp) occurs. 24 h after temperature shift more than 90% of the cells still appear vital. Alterations in the organization of the mitochondrial cristae are the only ultrastructural changes observed in these cells. In contrast, 70% of the cells degenerate 24 h after a temperature shift from 25 degrees C to 37 degrees C and in the remaining cells mitochondria are rarely observed. These observations are discussed in relation to the production of hsp, the uncoupling of oxidative phosphorylation and the virulence of different strains of H. capsulatum.
authors.affiliation: Clinica delle Malattie Infettive, II. Facoltá di Medicina e Chirurgia, Universitá degli Studi di Napoli, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Borgia
authors.forename: A
authors.initials: A
authors.lastname: Tallarino
authors.forename: J
authors.initials: J
authors.lastname: Crowell
authors.forename: A
authors.initials: A
authors.lastname: Lambiase
authors.forename: S
authors.initials: S
authors.lastname: Cicciarello
authors.forename: L
authors.initials: L
authors.lastname: Reynaud
authors.forename: G
authors.initials: G
authors.lastname: Nasti
authors.forename: M
authors.initials: M
authors.lastname: Piazza
mesh_terms: D006360:Heat-Shock Proteins / Q000096:biosynthesis; D006658:Histoplasma / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure*; D006358:Hot Temperature; D010085:Oxidative Phosphorylation
chemical_list: D006360:Heat-Shock Proteins