The use of paramagnetic beads for the detection of major histocompatibility complex class I and class II antigens.
Specific immunoadsorbents were prepared using paramagnetic particles (Dynabeads), and their ability to immunoprecipitate major histocompatibility complex (MHC) Class I and Class II antigens compared with conventional protein A Sepharose immunoadsorbents. Lysates of lymphoblastoid cells provided the antigen source which were visualized by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Dynabeads were found to be as effective as protein A Sepharose immunoadsorbents at immunoprecipitating MHC Class I and Class II antigens, but had a much lower nonspecific binding capacity resulting in fewer interference bands and lower backgrounds.
Worlock
A J
AJ
Department of Microbiology and Immunology, Duke University Medical Center, Durham, NC 27710.
Sidgwick
A
A
Horsburgh
T
T
Bell
P R
PR
D002478:Cells, Cultured; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D007163:Immunosorbent Techniques; D007164:Immunosorbents; D008214:Lymphocytes / Q000276:immunology; D008280:Magnetics; D008863:Microspheres; D008970:Molecular Weight
D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D007164:Immunosorbents