Cell lineage specificity of expression of the murine transforming growth factor beta 3 and transforming growth factor beta 1 genes.
We have cloned a murine transforming growth factor (TGF) beta 3 complementary (cDNA) from normal tissue by low stringency screening of a testicular cDNA library with a TGF beta 1 probe. The coding domain of this TGF beta 3 cDNA agrees completely with the sequence reported for the TGF beta 3 cDNA isolated from the AKR-2B cell line, but the testicular clone uses a distinct and unusual polyadenylation signal resulting in an altered 3' untranslated domain. Northern blot hybridization analysis of gonadal tissues showed that both TGF beta 3 and TGF beta 1 mRNAs are detectable in the mouse testis and ovary. A detailed analysis of TGF beta 3 and TGF beta 1 gene expression in normal and germ cell-deficient male mice showed that the somatic cell compartment of the mouse testis expresses the usual-sized transcripts for both genes. However, a smaller (1.8-kilobase) TGF beta 1 mRNA is expressed selectively in male germ cells, and expression of this transcript was constitutive throughout the spermatogenic stages examined. This result demonstrates a new pattern of TGF beta 1 gene expression, consistent with cell lineage-specific transcriptional regulation during spermatogenesis.
Watrin
F
F
Department of Genetics and Development, Columbia University, College of Physicians and Surgeons, New York, New York 10032.
Scotto
L
L
Assoian
R K
RK
Wolgemuth
D J
DJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D007668:Kidney; D008168:Lung; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010053:Ovary; D013091:Spermatogenesis; D013737:Testis; D016212:Transforming Growth Factor beta / Q000235:genetics*
D016212:Transforming Growth Factor beta; D004247:DNA