Down regulation of lamins A and C by murine interferon beta.
Treatment of spontaneously differentiated PSMB embryonal carcinoma cells with murine interferon beta results in a transient decrease in the expression of the nuclear lamins A and C. Reduced levels of mRNAs were observed 4 h after the addition of interferon beta, with reductions in the polypeptides and assembled proteins within the nuclear lamina seen after 8 h of treatment. Expression of the 72-kDa (lamin A) and the 62-kDa (lamin C) polypeptides remained down regulated for 8 h, returning to control levels after 16 h of interferon treatment. The specificity of this response is indicated by the inhibitory action of a neutralizing antibody to interferon.
Alldridge
L C
LC
Department of Biology, University of Essex, Wivenhoe Park, Colchester, United Kingdom.
O'Farrell
M K
MK
Dealtry
G B
GB
D000818:Animals; D000906:Antibodies / Q000276:immunology; D015153:Blotting, Western; D054278:Embryonal Carcinoma Stem Cells; D005455:Fluorescent Antibody Technique; D007370:Interferon Type I / Q000276:immunology / Q000494:pharmacology*; D034904:Lamin Type A; D034882:Lamins; D051379:Mice; D014411:Neoplastic Stem Cells; D009687:Nuclear Proteins / Q000187:drug effects*
D000906:Antibodies; D007370:Interferon Type I; D034904:Lamin Type A; D034882:Lamins; D009687:Nuclear Proteins; C440308:lamin C