Aspirin induces morphological transformation to the secretory state in isolated rabbit parietal cells.
The morphological response of rabbit parietal cells to aspirin was evaluated by grading several ultra-structural features including the extent of the tubulovesicular system, intracellular secretory canaliculi, and microvilli. After exposure of isolated parietal cells and gastric glands to aspirin or histamine, there was an approximately twofold increase in the ratio of secretory to nonsecretory parietal cells, and depletion of extracellular Ca2+ abolished the aspirin-induced morphological changes. Morphometry in parietal cells showed that aspirin induced a sixfold increase in secretory canalicular membrane elaboration. Aspirin potentiated histamine-induced parietal cell respiration and aminopyrine uptake ratio but did not increase basal respiration or aminopyrine uptake, suggesting an apparent dissociation from aspirin-induced morphological changes.
Murthy
U K
UK
Department of Medicine, State University of New York Health Science Center, Syracuse.
Levine
R A
RA
D000818:Animals; D001241:Aspirin / Q000494:pharmacology*; D002118:Calcium / Q000502:physiology; D005753:Gastric Mucosa / Q000187:drug effects / Q000648:ultrastructure; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D010295:Parietal Cells, Gastric / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D011817:Rabbits
D006632:Histamine; D001241:Aspirin; D002118:Calcium