title: The tubulin genes of the human malaria parasite Plasmodium falciparum, their chromosomal location and sequence analysis of the alpha-tubulin II gene.
abstract: We report the isolation and sequencing of genomic clones encompassing the entire alpha-tubulin II gene from the human malaria parasite Plasmodium falciparum. This gene is closely related to, but significant different from the alpha-tubulin I gene that we have described previously. These two genes represent the entire complement of alpha-tubulin sequences in this organism and are expressed in a stage-specific manner. The alpha-II gene is present as a single copy and encodes a tubulin molecule with a predicted length of 450 amino acid residues (49.7 kDa). Like the alpha-I gene, it contains two introns, which are in identical positions to those of alpha-I, but are about one-third smaller. The deduced alpha-II protein is very similar to alpha-tubulin I (94.2% amino acid identity), except for notable differences across residues 40-45. In addition, unlike the great majority of alpha-tubulin genes (including alpha-I), alpha-II does not encode a terminal tyrosine residue. Using pulsed field gel electrophoresis we demonstrate that the two alpha-tubulin genes, together with the single beta-tubulin gene, are unlinked, all residing on different chromosomes. We assign alpha-I to chromosome 9, alpha-II to chromosome 4 and beta-tubulin to chromosome 10.
authors.affiliation: Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology (UMIST), U.K.
authors.forename: S P
authors.initials: SP
authors.lastname: Holloway
authors.forename: M
authors.initials: M
authors.lastname: Gerousis
authors.forename: C J
authors.initials: CJ
authors.lastname: Delves
authors.forename: P F
authors.initials: PF
authors.lastname: Sims
authors.forename: J G
authors.initials: JG
authors.lastname: Scaife
authors.forename: J E
authors.initials: JE
authors.lastname: Hyde
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D005796:Genes; D007438:Introns; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics*; D011110:Polymorphism, Genetic; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014404:Tubulin / Q000235:genetics*
chemical_list: D014404:Tubulin