[Plasmid R68.45 and S-a transduction by the temperate bacteriophage 59 of Erwinia carotovora 268].
Temperature bacteriophage 59 of Erwinia carotovera 268 had transduced extrachromosomal DNA: plasmids of R68.45 and S-a. Before plasmid transduction experiments the suitable donor strains of indicator culture Erwinia horticola 450 harbouring R68.45 and S-a were created. The frequency of plasmid R68.45 transfer from Pseudomonas putida to E. horticola 450-8 by conjugation was equal to 5 x 10(-8) per a donor cell and in the case of S-a--from E. coli C600 for the same recipient cells--was 2 x 10(-6). Bacteriophage 59 has transduced only separate markers of plasmid R68.45, since plasmid S-a is probably transduced by the phage as an intact unit.
Mukvich
N S
NS
Romaniuk
L V
LV
Maliuk
A G
AG
Shevchenko
T V
TV
Kishko
Ia G
IaG
D001435:Bacteriophages / Q000235:genetics*; D003227:Conjugation, Genetic / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D004885:Erwinia / Q000235:genetics; D005111:Extrachromosomal Inheritance / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D011549:Pseudomonas / Q000235:genetics; D014161:Transduction, Genetic / Q000235:genetics*
D004269:DNA, Bacterial
Transduktsiia plazmid R68.45 i S-a umerennym bakteriofagom 59 Erwinia carotovora 268.