The endothelium in early experimental contact hypersensitivity of the oral mucosa. Leukocyte-binding properties and ultrastructure.
Contact hypersensitivity is characterized by an early and specific diapedesis of mononuclear cells into the site of antigenic challenge. In order to study the functional and ultrastructural properties of the endothelium involved in the recruitment of leukocytes, Sprague-Dawley rats were skin sensitized to DNFB; and this was followed by challenge of the oral mucosa. In vitro binding of peripheral blood mononuclear cells to high endothelial venules in lymph nodes was highly specific but no affinity of peripheral blood mononuclear cells to the vessels was observed in normal oral mucosa or in early contact hypersensitivity. However, 10 days after repeated DNFB challenge, occasional vessels bound overlaid peripheral blood mononuclear cells. Ultrastructurally, we identified migration of mononuclear cells through small venules three h after challenge. The vessels involved, however, did not display morphological signs of activation reminiscent of high endothelial venules in lymph nodes. Mast cell degranulation was evident as early as 30 min after challenge, and a possible mechanism for mast cell-mediated leukocyte recruitment is discussed.
Warfvinge
G
G
Department of Oral Pathology, School of Dentistry, University of Lund, Malmö, Sweden.
Larsson
A
A
D000818:Animals; D002448:Cell Adhesion; D003877:Dermatitis, Contact / Q000473:pathology / Q000503:physiopathology*; D004195:Disease Models, Animal; D004729:Endothelium, Lymphatic / Q000503:physiopathology* / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000503:physiopathology* / Q000648:ultrastructure; D005260:Female; D066298:In Vitro Techniques; D007962:Leukocytes / Q000502:physiology*; D008297:Male; D008854:Microscopy, Electron; D009061:Mouth Mucosa / Q000503:physiopathology* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains