title: [Features of a protein component of the endotoxin from Yersinia pseudotuberculosis].
abstract: The protein moiety of endotoxin from Yersinia pseudotuberculosis was found to consist of two polypeptides with apparent molecular masses 40 and 14.5 kDa (4:1 w/w). The major protein (40 kDa) was isolated from the endotoxin pretreated with sodium deoxy cholate by gel chromatography on the Sephadex G-200 column. Comparative study of this protein and oligomeric form of porin from the outer membrane of Y. pseudotuberculosis using SDS--PAGE, velocity sedimentation, lipid bilayer experiments, chemical and serological analyses revealed their identity. The deoxycholate treatment of the endotoxin does not affect complexes of the major protein and LPS.
authors.forename: T F
authors.initials: TF
authors.lastname: Solov'eva
authors.forename: S I
authors.initials: SI
authors.lastname: Bakholdina
authors.forename: I M
authors.initials: IM
authors.lastname: Ermak
authors.forename: V A
authors.initials: VA
authors.lastname: Khomenko
authors.forename: L I
authors.initials: LI
authors.lastname: Fedoreeva
authors.forename: O D
authors.initials: OD
authors.lastname: Novikova
authors.forename: G M
authors.initials: GM
authors.lastname: Frolova
authors.forename: G N
authors.initials: GN
authors.lastname: Likhatskaia
authors.forename: Iu S
authors.initials: IuS
authors.lastname: Ovodov
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000737:chemistry*; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000737:chemistry; D015011:Yersinia pseudotuberculosis / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D001425:Bacterial Outer Membrane Proteins; D004731:Endotoxins; D008070:Lipopolysaccharides
vernacular_title: Kharakteristika belkovogo komponenta éndotoksina iz Yersinia pseudotuberculosis.