Pertussis toxin pretreatment affects opiate/nonopiate and stress-induced analgesia differently.
Intracerebroventricular injection of pertussis toxin (PTX, 1 microgram/rat) six days before the hot plate test abolished analgesia induced by central morphine. The toxin did not affect analgesia evoked by central neurotensin or ASU 1-7 eel calcitonin. PTX pretreatment also attenuated footshock-induced analgesia (FSIA) delivered to all four paws. When the shock was restricted to the front paws, PTX consistently lowered postshock tail flick latencies, but did not reduce analgesia resulting from shock delivered to the hind paws. It thus appears that PTX-sensitive G-proteins are an essential transduction step needed to initiate the molecular events underlying opiate analgesia evoked by either morphine or shock. In contrast, the signal transduction mechanism subsequent to the stimulation of neurotensin or calcitonin receptors, and to the nonopiate FSIA, appears not to involve PTX-sensitive G-proteins.
Parolaro
D
D
Institute of Pharmacology, Faculty of Sciences, School of Medicine, University of Milan, Italy.
Massi
P
P
Patrini
G
G
Rubino
T
T
Parenti
M
M
Gori
E
E
D000818:Animals; D002116:Calcitonin / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors; D004597:Electroshock; D007276:Injections, Intraventricular; D008297:Male; D008845:Microinjections; D009020:Morphine / Q000037:antagonists & inhibitors*; D009496:Neurotensin / Q000037:antagonists & inhibitors*; D010146:Pain / Q000503:physiopathology*; D010147:Pain Measurement; D037342:Pertussis Toxin; D051381:Rats; D011919:Rats, Inbred Strains; D013312:Stress, Physiological / Q000503:physiopathology*; D013997:Time Factors; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D010566:Virulence Factors, Bordetella; D009496:Neurotensin; D009020:Morphine; D002116:Calcitonin; D037342:Pertussis Toxin; C012755:elcatonin