Linkage analysis of the human dopamine beta-hydroxylase gene.
The human gene for dopamine beta-hydroxylase (D beta H) has been mapped to chromosome 9q34. Using polymerase chain reaction amplification of exon 11 of the D beta H gene followed by digestion of the reaction products with FnuDII (BstUI), we detected a low-frequency restriction fragment length polymorphism (RFLP). The CEPH panel of family DNAs was genotyped for this RFLP, enabling us to determine the linkage relationships between D beta H and four other loci previously mapped to human chromosome 9q. We obtained two-point recombination frequencies (theta) between D beta H and arginosuccinate synthetase (theta = 0, LOD = 7.37), the ABO blood group locus (theta = 0, LOD = 4.5), CRI-P111 (theta = 0, LOD = 2.1), and D9S31 (theta = .06, LOD = 2.81).
Perry
S E
SE
Department of Medicine, Vanderbilt University, Nashville, Tennessee 37232.
Summar
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Phillips
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JA
Robertson
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D001483:Base Sequence; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9; D004247:DNA; D004299:Dopamine beta-Hydroxylase / Q000235:genetics*; D005091:Exons; D008040:Genetic Linkage; D006801:Humans; D008126:Lod Score; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D004247:DNA; D004299:Dopamine beta-Hydroxylase