title: Use of high resolution thermal denaturation approach in preliminary identification of plant DNAs.
abstract: High resolution thermal denaturation (HRTD) profiles of the DNAs of six related millets, oat and rice showed several maxima and shoulders skewed towards the GC-rich side. Linear regression and correlation analyses exhibited no correlation between the repetitive DNA content/nuclear DNA content and the number of peaks, while there existed a very good correlation between delta T and repetitive DNA content/nuclear DNA content indicating that increase in repetitive DNA content has resulted in a greater sequence heterogeneity in these DNAs. In addition, specific melting characteristics of each of the eight plant DNAs were identified, which showed species specificity.
authors.affiliation: Division of Biochemical Sciences, National Chemical Laboratory, Poona.
authors.forename: L S
authors.initials: LS
authors.lastname: Kumar
authors.forename: M N
authors.initials: MN
authors.lastname: Sainani
authors.forename: P K
authors.initials: PK
authors.lastname: Ranjekar
authors.forename: V R
authors.initials: VR
authors.lastname: Patwardhan
mesh_terms: D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D006358:Hot Temperature; D009691:Nucleic Acid Denaturation; D010944:Plants / Q000235:genetics*; D013045:Species Specificity
chemical_list: D004247:DNA