Effect of L-arginine on haemoglobin-induced inhibition of endothelium-dependent relaxation of isolated cerebral arteries.
The effects of Oxy-haemoglobin were investigated in the rat isolated basilar artery and compared to Methylene blue and NG-Nitro-L-Arginine (NOLAG), as these compounds interfere with the relaxation induced by the endothelium-derived relaxing factor. Acetylcholine induced a concentration-related relaxation of serotonin-precontracted arteries which was further enhanced by the application of L-arginine. Similarly, the spasmolytic activity of L-arginine was enhanced by acetylcholine. Oxy-haemoglobin, Methylene blue and NOLAG induced an increase of resting tension and inhibited acetylcholine-induced relaxation. Furthermore, inhibition of acetylcholine-induced relaxation by Oxy-haemoglobin and NOLAG but not that by Methylene blue was partially reversed by L-arginine.
Schilling
L
L
Department of Physiology, University of Munich, Federal Republic of Germany.
Parsons
A A
AA
Mackert
J R
JR
Wahl
M
M
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology*; D002536:Cerebral Arteries / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000187:drug effects*; D006454:Hemoglobins / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D008751:Methylene Blue / Q000494:pharmacology; D019335:Nitroarginine; D009994:Osmolar Concentration; D010108:Oxyhemoglobins / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000494:pharmacology; D014661:Vasoconstriction; D014664:Vasodilation
D006454:Hemoglobins; D010108:Oxyhemoglobins; D019335:Nitroarginine; D012701:Serotonin; D001120:Arginine; D000109:Acetylcholine; D008751:Methylene Blue