Electrophoresis of nonreduced IgM and IgG monoclonal antibodies on mixed agarose-polyacrylamide gels.
A gel matrix composed of 0.5% agarose and 2.5% polyacrylamide allows the parallel electrophoretic separation of nonreduced IgM and IgG monoclonal antibodies. The mobilities of IgM and IgG differ at pH 9.5, which enables a preliminary Ig class designation. It allows rapid detection of incomplete cloning if both IgM and IgG are present. A semiquantitative evaluation of nonreduced IgM in ascitic fluid is possible as well.
Slavícková
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Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
D000911:Antibodies, Monoclonal / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007075:Immunoglobulin M