Nucleolar organizer regions in meningiomas. Correlation with histopathologic malignancy grading, DNA cytometry and clinical outcome.
Eighty-one meningiomas (63 grade I, 9 grade II and 9 grade III) and 2 meningeal sarcomas (grade IV) were investigated by a simple one-step silver staining for nucleolar organizer region (NOR)-associated proteins (AgNOR technique) and by DNA cytometry. The number of NORs per cell and the NOR area per cell were correlated with the histopathologic grading, as were the 5c exceeding rate and the 2c deviation index (2cDI) obtained by DNA cytometry. The differences in NOR parameters were only significant (at P less than .001) between grades I and II; P was less than .05 for the 2cDI between grades I and II. No significant differences between grades II and III were found. Among recurrent tumors, the AgNOR technique revealed the proliferative potential in 8 of 11 tumors studied, whereas DNA cytometry failed to recognize malignant features in 8 of 10 tumors investigated.
Plate
K H
KH
Department of Neuropathology, Philipps Univeristy, Marburg, Federal Republic of Germany.
Ruschoff
J
J
Mennel
H D
HD
D004273:DNA, Neoplasm / Q000032:analysis*; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D008577:Meningeal Neoplasms / Q000737:chemistry / Q000145:classification / Q000473:pathology / Q000648:ultrastructure*; D008579:Meningioma / Q000737:chemistry / Q000145:classification / Q000473:pathology / Q000648:ultrastructure*; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D011379:Prognosis; D012834:Silver; D014944:World Health Organization
D004273:DNA, Neoplasm; D012834:Silver