Interleukin-1 beta stimulates fibroblast elastase activity.
We report the stimulation of elastase activity of human skin fibroblasts by interleukin-1 beta (IL-1). Elastase activity was determined by the hydrolysis of the synthetic substrate succinoyl trialanine paranitroanilide (Suc(Ala3)NA). Recombinant human IL-1 beta induced a concentration-dependent increase in the elastase activity of fibroblasts. The supernatants of keratinocyte cultures that contained IL-1/ETAF showed similar stimulatory activity.
Croute
F
F
INSERM U.209, Hôpital E. Herriot, Lyon, France.
Delaporte
E
E
Bonnefoy
J Y
JY
Fertin
C
C
Thivolet
J
J
Nicolas
J F
JF
D000328:Adult; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004817:Epidermis / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism* / Q000494:pharmacology; D015603:Keratinocytes / Q000378:metabolism; D010196:Pancreatic Elastase / Q000378:metabolism*; D012867:Skin / Q000187:drug effects / Q000201:enzymology*
D007375:Interleukin-1; D010196:Pancreatic Elastase