[Novel PCR methods for detection of HIV proviruses using digoxigenin labelled probes--Southern blot and dot blot].
Polymerase chain reaction (PCR) is useful for diagnosis of HIV infection in the silent stage, for HIV transmission from mother to child and for existence of HIV in blood and blood products. We developed a simple, rapid and safe PCR method by using digoxigenin labelled probes. Furthermore, we developed a semi-quantitative dot blot method. These methods will be helpful for diagnosis.
Yamaguchi
K
K
Department of Internal Medicine, Juntendo University School of Medicine.
Ushijima
H
H
Tsuchie
H
H
Matsumoto
T
T
Matsuda
J
J
Kitamura
T
T
D015139:Blotting, Southern / Q000379:methods*; D015342:DNA Probes; D004076:Digoxigenin; D006678:HIV / Q000302:isolation & purification; D015658:HIV Infections / Q000175:diagnosis*; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011533:Proviruses / Q000302:isolation & purification
D015342:DNA Probes; D004076:Digoxigenin