title: [Hybridase cleavage of RNA. II. Automatic synthesis of mixed oligonucleotide probes].
abstract: Several oligo(ribodeoxyribo)nucleotides to be used as probes in the RNase H-induced hydrolyses of 5S ribosomal RNA E. coli were synthesized on the Victoria-4M automatic gene synthesizer by the phosphoramidite approach, which allows for introducing ribonucleotides into any position of the oligomer. 2'-Hydroxy function was protected by tert-butyldimethylsilyl group whose hydrophobicity simplified isolation of the oligonucleotides by reverse-phased HPLC.
authors.forename: E A
authors.initials: EA
authors.lastname: Romanova
authors.forename: T S
authors.initials: TS
authors.lastname: Oretskaia
authors.forename: V V
authors.initials: VV
authors.lastname: Sukhomlinov
authors.forename: N F
authors.initials: NF
authors.lastname: Krynetskaia
authors.forename: V G
authors.initials: VG
authors.lastname: Metelev
authors.forename: Z A
authors.initials: ZA
authors.lastname: Shabarova
mesh_terms: D001345:Autoradiography; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000138:chemical synthesis*
chemical_list: D015345:Oligonucleotide Probes
vernacular_title: Gibridaznoe rasshcheplenie RNK. II. Avtomaticheskiĭ sintez smeshannykh oligonukleotidnykh zondov.