Characterization of polymorphic loci on a telomeric fragment of DNA from the long arm of human chromosome 7.
The 240-kb yeast artificial chromosome (YAC) HTY146 (D7S427) containing the telomere from the q arm of human chromosome 7 was subcloned into the cosmid vector sCOS-1. Cosmid subclones were screened for DNA polymorphisms by Southern blot analysis of restriction digests of DNA from random individuals. Four distinct polymorphisms were characterized. These markers provide a resource for defining the end of the genetic map for the long arm of human chromosome 7.
Dietz-Band
J
J
Genetics Group, Los Alamos National Laboratory, New Mexico 87545.
Riethman
H
H
Hildebrand
C E
CE
Moyzis
R
R
D015139:Blotting, Southern; D015825:Chromosomes, Fungal; D002897:Chromosomes, Human, Pair 7; D003001:Cloning, Molecular; D003360:Cosmids; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D015723:Gene Library; D015894:Genome, Human; D006801:Humans; D011110:Polymorphism, Genetic
D015342:DNA Probes; D004247:DNA