Immunohistochemical localization of glycated protein in diabetic rat kidney.
The localization of glycated protein in the kidney of diabetic rats was examined immunohistochemically with antiserum against glucitol-lysine. In diabetic rats the brush border and basement membrane of the proximal convoluted tubules were strongly immunoreactive with the antiserum but in control rats, only the brush border was weakly reactive. The immunoreactive tubules were more abundant in diabetic rats. No immunoreaction was found in any other structures in the kidney. Glycation of the proximal convoluted tubules may be an alteration in diabetic nephropathy.
Yano
M
M
Department of Laboratory Medicine, School of Medicine, University of Tokushima, Japan.
Mizuno
A
A
Shima
K
K
D000818:Animals; D000906:Antibodies; D001485:Basement Membrane / Q000648:ultrastructure; D003921:Diabetes Mellitus, Experimental / Q000473:pathology*; D006031:Glycosylation; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000473:pathology*; D008239:Lysine / Q000031:analogs & derivatives* / Q000032:analysis; D008297:Male; D008871:Microvilli / Q000648:ultrastructure; D009928:Organ Specificity; D011506:Proteins / Q000032:analysis*; D051381:Rats
D000906:Antibodies; D007106:Immune Sera; D011506:Proteins; C034247:glucitollysine; D008239:Lysine