Isolation of genes highly expressed in early and late stages of Friend virus-induced erythroleukemia in mice.
We isolated two genes that are highly expressed in Friend erythroleukemia. The first one, expressed at an extremely high level in a late-stage tumorigenic cell line F5-5, was found to be a species of intracisternal A-particle. Most of those genomes in F5-5 DNA were not amplified nor rearranged compared with those in the normal cell DNA, suggesting transcriptional activation. Another late-stage cell line T3K showed similar results. The second gene, highly expressed in early-stage cells but weaker in late-stage cells, was identified as one of the heme-synthesizing enzymes, uroporphyrinogen decarboxylase. Usefulness of these two genes for the analysis of erythroid transformation and differentiation will be discussed.
Misawa
Y
Y
Institute of Medical Science, University of Tokyo, Japan.
Tojo
A
A
Shibuya
M
M
D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D002262:Carboxy-Lyases / Q000235:genetics*; D002460:Cell Line; D004273:DNA, Neoplasm / Q000032:analysis / Q000302:isolation & purification*; D005622:Friend murine leukemia virus; D005784:Gene Amplification; D007426:Genes, Intracisternal A-Particle; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology / Q000235:genetics*; D051379:Mice; D011519:Proto-Oncogenes; D012334:RNA, Neoplasm / Q000032:analysis; D014575:Uroporphyrinogen Decarboxylase / Q000235:genetics*
D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D002262:Carboxy-Lyases; D014575:Uroporphyrinogen Decarboxylase