[Cloning of the Streptococcus thermophilus beta-galactosidase gene and its expression in Escherichia coli and Bacillus subtilis cells].
The beta-galactosidase gene from the chromosome of Streptococcus thermophilus, strain 6 kb, has been cloned on a vector plasmid pBR322. The corresponding gene has been found to be located on the Pst1 DNA fragment. The restriction map of this 6 kb fragment has been constructed. The shortening of the DNA fragment carrying the beta-galactosidase gene has been achieved by digestion of the recombinant derivative of pBR322 by the restriction endonuclease Sau3A under the conditions of incomplete hydrolysis. The obtained fragments have been cloned into the BamHI site in the berepliconed shuttle vector pCB20 for grampositive and gramnegative bacteria. The obtained recombinant plasmids contained the beta-galactosidase gene in the inserted fragments of different length. Expression of the cloned beta-galactosidase gene in Escherichia coli and Bacillus subtilis cells has been studied.
Molotov
S V
SV
Duzhiĭ
D E
DE
Danilevich
V N
VN
Sukhodolets
V V
VV
D001412:Bacillus subtilis / Q000235:genetics; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005696:Galactosidases / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D010957:Plasmids; D015183:Restriction Mapping; D013291:Streptococcus / Q000201:enzymology / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics*
D004269:DNA, Bacterial; D005696:Galactosidases; D001616:beta-Galactosidase
Klonirovanie gena beta-galaktozidazy Streptococcus thermophilus i ego ékspressiia v kletkakh Escherichia coli i Bacillus subtilis.