Differential requirement for protein synthesis in cytolysis mediated by class I and class II MHC-restricted cytotoxic T cells.
Antigen-specific cytolysis by major histocompatibility complex (MHC) class II-restricted cloned T-cell lines was found to be dependent on protein synthesis. Cytolysis by both polyclonal short-term and long-term class I-restricted cytotoxic T lymphocytes (CTL) was almost completely insensitive to inhibitors of protein synthesis. Kinetic studies with class II-restricted CTL indicated that approximately 2-3 hr after initiation of activation, resistance to inhibitors of protein synthesis was acquired. This strongly suggests that either a cytotoxic effector molecule or an intermediary important in the delivery of such a molecule is synthesized rapidly after activation in class II-restricted CTL, whilst class I-restricted CTL have no such requirement.
Tite
J P
JP
Department of Molecular Biology, Wellcome Biotech, Beckenham, Kent, U.K.
D000818:Animals; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects / Q000276:immunology*; D003609:Dactinomycin / Q000494:pharmacology; D005260:Female; D005802:Genes, MHC Class II / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D008285:Major Histocompatibility Complex / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D014176:Protein Biosynthesis; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism
D003609:Dactinomycin; D003513:Cycloheximide