Adrenergic and calcium-mediated subcellular redistribution of protein kinase C in primary neuronal cultures.
Incubation of primary neuronal cultures prepared from the brains of neonatal rats with 50 microM epinephrine resulted in the transient redistribution of protein kinase C from the cytosol to the particulate fraction. This effect occurred after 1 and 5 min of incubation and resulted in a decrease in cytosolic protein kinase C activity with a corresponding increase in particulate protein kinase C of approximately 30% and 15%, respectively. The epinephrine-stimulated translocation of protein kinase C was blocked by 1 microM prazosin indicating the involvement of alpha 1-adrenergic receptors. Further, inclusion of 0.1 microM Ca2+ in the homogenization buffer was found to significantly enhance the binding of protein kinase C to cellular membranes prepared from neuronal cultures. These results indicate that alpha 1-adrenergic receptors in neuronal brain cell cultures are linked to the activation of protein kinase C and that the mobilization of Ca2+ may enhance this effect.
Kalberg
C J
CJ
Department of Physiology, College of Medicine, University of Florida, Gainesville 32610.
Sumners
C
C
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000201:enzymology; D002122:Calcium Chloride / Q000494:pharmacology*; D002458:Cell Fractionation / Q000379:methods; D002478:Cells, Cultured; D003600:Cytosol / Q000201:enzymology; D004533:Egtazic Acid / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology*; D007700:Kinetics; D009474:Neurons / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D011224:Prazosin / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D004533:Egtazic Acid; D011493:Protein Kinase C; D002122:Calcium Chloride; D013755:Tetradecanoylphorbol Acetate; D011224:Prazosin; D004837:Epinephrine