title: Conditions for the extraction of protease-free LMG proteins from calf thymus.
abstract: Calf thymus LMG proteins are known to be susceptible to a wide variety of chromatin-associated proteases. We have investigated the use of different inhibitors to protect LMG chromosomal proteins from proteolytic degradation. In this work we have developed a preparative method for the extraction of LMG proteins from purified chromatin which provides an LMG protein extract devoid of proteolytic activity.
authors.affiliation: Laboratoire de Biochimie et Radiobiochimie, Faculté des Sciences, Université de Nantes, France.
authors.forename: J P
authors.initials: JP
authors.lastname: Durand
authors.forename: F
authors.initials: F
authors.lastname: Goudard
authors.forename: J
authors.initials: J
authors.lastname: Pieri
authors.forename: J M
authors.initials: JM
authors.lastname: Walker
mesh_terms: D000818:Animals; D002417:Cattle; D002843:Chromatin / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification*; D010450:Endopeptidases / Q000302:isolation & purification; D011480:Protease Inhibitors; D013950:Thymus Gland / Q000737:chemistry*
chemical_list: D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D011480:Protease Inhibitors; D010450:Endopeptidases