[Formation of interleukin-2 and interferon alpha by mononuclear leukocytes of swine after in vitro stimulation with different virus preparations].
A new model was established to investigate the release of cytokines by porcine peripheral mononuclear leukocytes (MNL). Thereafter, this model is suitable to test paramunity inducers (biological response modifiers) and might replace animal models. The paramunity inducers Pind AVI and Pind ORF as well as other preparations of pox- and paramyxoviruses were employed. Production of both Interleukin 2 (IL2) and Interferon alpha (IFN alpha) was evaluated. Virus containing fluids induced an increase in IL2 activity (CTLL-2 proliferation assay) and IFN alpha (MDBK-cell line, VSV-virus) was noted. The release of IFN alpha and IL2 is caused by viral components. Pind AVI and Pind ORF induce production of IFN alpha in porcine MNL to a similar amount as ND-virus does. All virus preparations caused an increase in IL2 activity in supernatants of resting as well as mitogen-activated MNL without prior sensitization of the cells. The highest IL2-activity was measured after co-stimulation of the MNL with Concanavalin A and ND-virus.
Steinmassl
M
M
Institut für Med. Mikrobiologie, Infektions-und Seuchenlehre, Tierärztlichen Fakultät der Ludwig-Maximilians-Universität.
Wolf
G
G
D000818:Animals; D001688:Biological Products / Q000494:pharmacology; D002478:Cells, Cultured; D007370:Interferon Type I / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D011837:Radiation-Protective Agents / Q000494:pharmacology; D013552:Swine; D014780:Viruses / Q000276:immunology*
D001688:Biological Products; D007370:Interferon Type I; D007376:Interleukin-2; C028621:PIND-AVI; C044867:PIND-ORF; D011837:Radiation-Protective Agents
Bildung von Interleukin-2 und Interferon alpha durch mononukleäre Leukozyten des Schweines nach in vitro-Stimulation mit verschiedenen Viruspräparaten.