Molecular cloning of porcine parvovirus DNA for the purpose of obtaining viral antigen synthesis in bacteria.
An expression of porcine parvovirus 1.05 kb DNA fragment was obtained in Escherichia coli under the control both of Pr-lambda and lac-promoters. The product of expression under Pr-control was demonstrated to show PPV-specific antigenic properties. Its electrophoretic mobility corresponded to a molecular weight of approximately 45 KD.
Zaberezhny
A D
AD
All-Union Institute of Experimental Veterinary Medicine, Moscow, UdSSR.
Konorova
A L
AL
D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis*; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D010321:Parvoviridae / Q000235:genetics*; D013552:Swine
D000956:Antigens, Viral; D004279:DNA, Viral