[The development of an immunoenzyme method for determining human secretory IgA].
The results of the work on the development of an enzyme immunoassay (EIA) system for the determination of secretory IgA (S-IgA) are presented. A first, S-IgA was isolated from human colostrum and used as the basis for obtaining biologically active immunosorbent; then antibodies to S-IgA were isolated and the specific conjugate was obtained. The determination of S-IgA was carried out by the method of sandwich EIA. The newly developed EIA system permitted the determination of S-IgA only, giving no positive reactions with serum immunoglobulins. The data thus obtained make it possible to regard this assay system as specific, sensitive and suitable for further trials.
Iankina
N F
NF
Semenova
G V
GV
Shkurina
E A
EA
D000918:Antibody Specificity; D002850:Chromatography, Gel; D003126:Colostrum / Q000276:immunology; D006801:Humans; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D007071:Immunoglobulin A, Secretory / Q000032:analysis* / Q000302:isolation & purification
D007071:Immunoglobulin A, Secretory
Razrabotka immunofermentnogo metoda dlia opredeleniia sekretornogo IgA cheloveka.