Aortic endothelial cell damage induced by beta-VLDL and macrophages in vitro.
We performed an in vitro study to assess damage to swine aortic endothelial cells by rabbit beta-VLDL and/or rabbit peritoneal macrophages. Incubation of cultured aortic endothelial cells with beta-VLDL, macrophages, or macrophage lysate induced endothelial cell damage time- and dose-dependently as estimated by [3H]adenine release. Incubation of endothelial cells with both beta-VLDL and macrophages produced a synergistic effect on the increase of [3H]adenine release. Pretreatment of the endothelial cells with some kinds of antioxidants (probucol 50 micrograms/ml, vitamin E 50 microM, superoxide dismutase-polyethylene glycol 0.5 mg/ml, or catalase-polyethylene glycol 0.5-1.0 mg/ml) significantly prevented the endothelial damage by beta-VLDL or macrophage lysate. We conclude that beta-VLDL and/or macrophages could induce endothelial cell damage and that some kinds of antioxidants could prevent it.
Tanimura
N
N
First Department of Pathology, Miyazaki Medical College, Japan.
Asada
Y
Y
Hayashi
T
T
Kisanuki
A
A
Sumiyoshi
A
A
D000225:Adenine / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001013:Aorta, Thoracic / Q000378:metabolism / Q000473:pathology; D002374:Catalase / Q000494:pharmacology; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D008079:Lipoproteins, VLDL / Q000502:physiology* / Q000633:toxicity; D008264:Macrophages / Q000502:physiology*; D008297:Male; D011092:Polyethylene Glycols / Q000494:pharmacology; D011341:Probucol / Q000494:pharmacology; D011817:Rabbits; D013482:Superoxide Dismutase / Q000494:pharmacology; D013552:Swine; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D008079:Lipoproteins, VLDL; C070295:catalase-polyethylene glycol; D014810:Vitamin E; D011092:Polyethylene Glycols; D002374:Catalase; D013482:Superoxide Dismutase; C063471:polyethylene glycol-superoxide dismutase; D000225:Adenine; D011341:Probucol