The prevention of diabetic retinal and renal basement membrane thickening in mice by islet cell allografts.
The effect of fetal islet cell allografts on retinal capillary (RCBMT) and glomerular (GBMT) basement membrane thickening in diabetic mice was studied, as well as the effect of diabetes on the anterior lens capsule and the inner lamina of Descemet's membrane. CBA mice rendered diabetic with streptozotocin received fetal pancreatic islet cell grafts from C3H and BALB/c donors four weeks after induction of diabetes with an additional late transplant group receiving C3H grafts after six months of diabetes. After 12 months animals with successful grafts were examined for RCBMT along with untreated diabetics (UD, n = 4) and normals (N, n = 9). Untreated diabetics had significantly increased RCBMT (167.1 +/- 12.1 vs 129.2 +/- 24.3 nm, p = 0.05) and GBMT (278 +/- 11 vs 248 +/- 6 nm, p = 0.004) compared to normals. All graft recipients had RCBMT similar to normal and significantly less than untreated diabetics: BALB/c (n = 7), 123.8 +/- 21.0, p = 0.02; C3h early (n = 5), 126.6 +/- 21.7, p = 0.05; C3H late (n = 4), 134.4 +/- 16.9, p = 0.03. The same was true for GBMT; 250 +/- 10 nm, p = 0.02 for BALB/c recipients, 250 +/- 8 nm, p = 0.04 for early and 255 +/- 9 nm, p = 0.02 for late C3H recipients. GMBT in six month biopsies of normals and prospective late graft recipients were well below these levels.(ABSTRACT TRUNCATED AT 250 WORDS)
Gillies
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MC
Transplantation Unit, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.
Mandel
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TE
D000818:Animals; D001485:Basement Membrane / Q000473:pathology*; D001786:Blood Glucose; D001835:Body Weight; D046508:Culture Techniques; D003886:Descemet Membrane / Q000473:pathology; D003921:Diabetes Mellitus, Experimental / Q000601:surgery*; D003930:Diabetic Retinopathy / Q000473:pathology / Q000517:prevention & control*; D016381:Islets of Langerhans Transplantation; D007678:Kidney Glomerulus / Q000473:pathology*; D007903:Lens Capsule, Crystalline / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D012171:Retinal Vessels / Q000473:pathology*; D014184:Transplantation, Homologous
D001786:Blood Glucose