Structural, functional, and subunit assembly properties of hemoglobin Attleboro [alpha 138 (H21) Ser----Pro], a variant possessing a site maturation at a critical C-terminal residue.
Hemoglobin Attleboro, a new alpha-chain variant with a substitution of proline for serine at position 138 (H21), was found to be a noncooperative high-affinity hemoglobin (P50 = 0.26 mmHg at pH 7 and 20 degrees C) which lacked an alkaline Bohr effect. Addition of 2,3-diphosphoglycerate (DPG) or inositol hexaphosphate (IHP) led to a decrease in oxygen affinity but to no alteration in either Bohr effect or cooperativity. Ligand binding kinetics studies revealed an overall rate of oxygen dissociation at pH 7.0 and 20 degrees C that was 2.7-fold slower than that for Hb A. At pH 8.5, the kinetic profile was identical with that at pH 7, confirming the absence of a Bohr effect for this variant hemoglobin. Measurement of the rate of oxygen dissociation with carbon monoxide replacement indicated a lack of cooperativity. Sedimentation velocity experiments yielded s20,w values of 2.8 and 4.3 for 65 microM solutions of oxyhemoglobins Attleboro and A, respectively (indicating an enhancement in the oxy dimer population of this variant). Studies of the carbon monoxide combination of this variant revealed an association rate 20-fold faster than that for Hb A; only in the presence of a 1000-fold molar excess of IHP was there a significant reduction in the overall rate. Rapid-scan and traditional stopped-flow experiments conducted in the Soret Soret region demonstrated an alteration in the structure and rate of assembly of the deoxy tetramer of Hb Attleboro relative to that of Hb A. The abnormal properties of this hemoglobin variant can be attributed to major perturbations in the C-terminal region.
McDonald
M J
MJ
Department of Chemistry, University of Lowell, Massachusetts 01854.
Michalski
L A
LA
Turci
S M
SM
Guillette
R A
RA
Jue
D L
DL
Johnson
M H
MH
Moo-Penn
W F
WF
D000595:Amino Acid Sequence; D002675:Child, Preschool; D005260:Female; D006455:Hemoglobins, Abnormal / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009154:Mutation; D010100:Oxygen / Q000378:metabolism; D010108:Oxyhemoglobins / Q000378:metabolism; D004856:Postural Balance; D011392:Proline / Q000378:metabolism; D011485:Protein Binding; D012694:Serine / Q000378:metabolism
D006455:Hemoglobins, Abnormal; D008024:Ligands; D046911:Macromolecular Substances; D010108:Oxyhemoglobins; C063394:hemoglobin Attleboro; D012694:Serine; D011392:Proline; D010100:Oxygen