Flow cytometry resonance energy transfer suggests an association between low-affinity interleukin 2 binding sites and HLA class I molecules.
Using flow cytometry energy transfer we have studied the sterical proximity of interleukin 2 receptors and the heavy chain of the major histocompatibility complex at the surface of normal lymphocytes. Our data suggest that class I molecules may be part of a low-affinity interleukin 2 receptor multimolecular complex, where the MHC class I heavy chain is in close proximity to the actual interleukin 2 binding site, in contrast to the light chain (beta 2-microglobulin).
Harel-Bellan
A
A
Laboratory of Immunoregulation, National Cancer Institute, Frederick, MD 21701.
Krief
P
P
Rimsky
L
L
Farrar
W L
WL
Mishal
Z
Z
D000906:Antibodies / Q000378:metabolism; D001665:Binding Sites; D002462:Cell Membrane / Q000032:analysis; D004735:Energy Transfer; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D015375:Receptors, Interleukin-2 / Q000032:analysis*; D012235:Rhodamines; D013601:T-Lymphocytes / Q000032:analysis*; D013861:Thiocyanates; D001613:beta 2-Microglobulin / Q000276:immunology
D000906:Antibodies; D005452:Fluoresceins; D005456:Fluorescent Dyes; D015395:Histocompatibility Antigens Class I; D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D012235:Rhodamines; D013861:Thiocyanates; D001613:beta 2-Microglobulin; C005358:tetramethylrhodamine; D016650:Fluorescein-5-isothiocyanate