The effect of complete carcinogens on intercellular transfer of lucifer yellow in fibroblast culture.
The effect on permeability of gap junctions of complete powerful carcinogens, 3-methylcholanthrene (MC), 7,12-dimethylbenz(a)anthracene (DMBA), ethyl methanesulfonate (EMS), and weak carcinogens, benz(a)anthracene (BA), benzo(e)pyrene (B(e)P) as well as the aryl-hydroxylase inhibitor 7,8-benzoflavone (7,8-BF) has been studied with the use of a dye-coupling technique and transformed Djungarian hamster DM15 fibroblasts. MC, EMS and 7,8-BF were found to exert a strong inhibitory effect on cell-to-cell dye transfer. BA and DMBA had the uncoupling activity only in 2 out of 4 experiments. B(e)P was not shown to affect LY transfer between DM15 cells. The uncoupling effect of MC, 7,8-BF and EMS (only when EMS used at the concentration of 600 micrograms/ml but not 1000 micrograms/ml) appeared reversible. The causes of failure to detect DMBA and B(e)P effects on gap junctions are discussed.
Budunova
I V
IV
Laboratory of Carcinogen Screening Methods, All-Union Cancer Research Center of the AMS USSR, Moscow.
Mittelman
L A
LA
Belitsky
G A
GA
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D000818:Animals; D001551:Benz(a)Anthracenes / Q000633:toxicity; D001564:Benzo(a)pyrene / Q000633:toxicity; D001571:Benzoflavones / Q000633:toxicity; D002273:Carcinogens / Q000633:toxicity*; D002461:Cell Line, Transformed; D002463:Cell Membrane Permeability / Q000187:drug effects* / Q000502:physiology; D002472:Cell Transformation, Viral; D006224:Cricetinae; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D005347:Fibroblasts / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure; D007365:Intercellular Junctions / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D007546:Isoquinolines / Q000378:metabolism*; D008748:Methylcholanthrene / Q000633:toxicity
D001551:Benz(a)Anthracenes; D001571:Benzoflavones; D002273:Carcinogens; D007546:Isoquinolines; D001564:Benzo(a)pyrene; D008748:Methylcholanthrene; D015127:9,10-Dimethyl-1,2-benzanthracene; C017475:lucifer yellow; D005020:Ethyl Methanesulfonate