A simple technique for preparation of bone marrow or peripheral blood buffy coat cells for electron microscopy.
A simple and rapid method of processing ethylene-diamine-tetra-acetic acid anticoagulated peripheral blood or aspirated bone marrow for electron microscopy is described. The resultant buffy coat pellet is easily processed into epoxy resin. Semithin sections (1 mu) stained with 1% toluidine blue reveal the various stratified cell layers allowing convenient selection for ultramicrotomy and ultrastructural evaluation.
Mills
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AE
Department of Pathology, Red Cross War Memorial Children's Hospital, Cape Town, South Africa.
Emms
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M
Licata
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SG
D000208:Acute Disease; D001707:Biopsy, Needle; D001800:Blood Specimen Collection; D001853:Bone Marrow / Q000648:ultrastructure*; D004492:Edetic Acid; D006801:Humans; D007938:Leukemia / Q000473:pathology; D007962:Leukocytes / Q000648:ultrastructure*; D008214:Lymphocytes / Q000648:ultrastructure; D008854:Microscopy, Electron; D009447:Neuroblastoma / Q000648:ultrastructure
D004492:Edetic Acid