The development of a radioimmunoassay system for testosterone (T) and dihydrotestosterone (DHT). Part 2. The preparation of antisera to T.
Testosterone-3-O-carboxymethyl-oxime derivative was synthetized and coupled to bovine serum albumin (BSA). The T-3-CMO-BSA conjugate homogenized with Freund's adjuvant used as immunogen was injected multiple sites in rabbits. The antisera collected were characterized in a radioimmunological system, separation with dextran-charcoal using 125I-Testosterone as tracer. The antibody titres varied from one animal to another. The titre of anti-T serum selected for RIA was 1: 10(4)-1: 2 X 10(4) (initial dilution). All anti-T sera 100 percent crossreacted with 5 alpha-dihydrotestosterone but nonsignificant interference was observed with other C19, C21 and C18 steroids. The affinity constant of the selected anti-T serum was in the range 1.4-1.9 X 10(9) litres/mole. The data so far published on the antisera toward testosterone are reviewed. We conclude that the selected anti-T-3-CMO-BSA serum may provide assays for testosterone with potential for clinical applications.
Simionescu
L
L
C. I. Parhon Institute of Endocrinology, Bucharest, Romania.
Neacşu
E
E
Zimel
A
A
Caragheorgheopol
A
A
D000818:Animals; D000915:Antibody Affinity; D000918:Antibody Specificity; D013196:Dihydrotestosterone / Q000097:blood* / Q000276:immunology; D005620:Freund's Adjuvant / Q000008:administration & dosage; D007106:Immune Sera / Q000032:analysis / Q000302:isolation & purification*; D007114:Immunization / Q000379:methods; D007457:Iodine Radioisotopes; D008297:Male; D011817:Rabbits; D011863:Radioimmunoassay / Q000295:instrumentation / Q000379:methods*; D013739:Testosterone / Q000097:blood* / Q000276:immunology; D013997:Time Factors
D007106:Immune Sera; D007457:Iodine Radioisotopes; D013196:Dihydrotestosterone; D013739:Testosterone; D005620:Freund's Adjuvant