Surface Listeria monocytogenes carbohydrate-binding components revealed by agglutination with neoglycoproteins.
Carbohydrate-binding components were shown to be present at the surface of Listeria monocytogenes by means of a panel of neoglycoproteins using direct agglutination. These lectin-like components bind on neoglycoproteins bearing D-glucosamine, L-fucosylamine, or para-amino-phenyl-alpha-D-mannopyrannoside residues. The interactions were inhibited by the carbohydrate moieties specific to the neoglycoproteins. The protein nature of the lectin-like components of L. monocytogenes was ascertained by the loss of carbohydrate-binding capacity following protease treatment.
Cottin
J
J
UFR Médecine-Pharmacie-Laboratoire de Bactériologie, Angers, France.
Loiseau
O
O
Robert
R
R
Mahaza
C
C
Carbonnelle
B
B
Senet
J M
JM
D000372:Agglutination Tests; D050260:Carbohydrate Metabolism; D004492:Edetic Acid / Q000494:pharmacology; D037102:Lectins / Q000378:metabolism; D008089:Listeria monocytogenes / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D011402:Pronase / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
D037102:Lectins; D008562:Membrane Glycoproteins; D004492:Edetic Acid; D014357:Trypsin; D011402:Pronase