Dimethylarginine:pyruvate aminotransferase in rats. Purification, properties, and identity with alanine:glyoxylate aminotransferase 2.
Dimethylarginine:pyruvate aminotransferase, which plays a role in the metabolism of dimethylarginines, has been purified to homogeneity from rat kidney. The enzyme has a molecular weight of approximately 200,000 and an isoelectric point at about pH 6.3. The enzyme consists of four similar subunits having a molecular weight of about 50,000. The enzyme catalyzes the effective transaminations of guanidino-N methylated L-arginines (e.g. NG,NG-dimethyl-L-arginine, NG,N'G-dimethyl-L-arginine and NG-monomethyl-L-arginine) and the alpha-amino group of L-ornithine to pyruvate or glyoxylate. The enzyme was always accompanied by the known alanine:glyoxylate amino-transferase activity with the ratios of their specific activities remaining constant during the purification steps. The physicochemical and immunological properties of the purified enzyme were shown to be identical with those of the isozyme of alanine:glyoxylate aminotransferase (EC 2.6.1.44), designated as alanine:glyoxylate aminotransferase 2 (Noguchi, T. (1987) in Peroxisomes in Biology and Medicine (Fahimi, H. D., and Sies, H., eds) pp. 234-243, Springer-Verlag, Heidelberg). The distribution profiles in tissues and the negative response to glucagon treatment further supported the identity of the two enzymes. The present data show that alanine:glyoxilate aminotransferase 2 functions in dimethylarginine metabolism in vivo in rats.
Ogawa
T
T
Department of Nutrition, School of Medicine, University of Tokushima, Japan.
Kimoto
M
M
Sasaoka
K
K
D000410:Alanine Transaminase / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002852:Chromatography, Ion Exchange; D005934:Glucagon / Q000494:pharmacology; D006038:Glyoxylates / Q000378:metabolism; D005779:Immunodiffusion; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D046911:Macromolecular Substances; D008970:Molecular Weight; D009928:Organ Specificity; D011773:Pyruvates / Q000378:metabolism; D051381:Rats; D013053:Spectrophotometry; D013379:Substrate Specificity; D000637:Transaminases
D006038:Glyoxylates; D046911:Macromolecular Substances; D011773:Pyruvates; D005934:Glucagon; D000637:Transaminases; D000410:Alanine Transaminase; C019635:Alanine-glyoxylate transaminase