Enhanced chemiluminescence ELISA for Listeria specific antigen in cerebrospinal fluid using an FITC-anti-FITC system.
An enzyme-linked immunosorbent assay (ELISA) is described for the detection of a soluble Listeria monocytogenes serogroup 4 antigen in cerebrospinal fluid samples (CSFs). In the ELISA an anti-Listeria monoclonal antibody, immobilized onto assay wells, was used to capture antigen from CSFs, the captured antigen was then reacted with a fluorescein isothiocyanate (FITC) conjugate of the same anti-Listeria antibody, which was detected with a horseradish peroxidase conjugate of a monoclonal antibody to FITC. The presence of antigen was detected by an enhanced chemiluminescence assay using a camera luminometer. Antigen was detected in the CSFs taken from five out of seven patients with culture proven L. monocytogenes serogroup 4 central nervous system infections, and in none of the CSFs taken from 25 other patients.
Samuel
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Division of Microbiological Reagents and Quality Control, Central Public Health Laboratory, Colindale, London, UK.
McLauchlin
J
J
Taylor
A G
AG
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000134:cerebrospinal fluid*; D004797:Enzyme-Linked Immunosorbent Assay; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D006801:Humans; D008089:Listeria monocytogenes / Q000145:classification / Q000276:immunology*; D008163:Luminescent Measurements; D008584:Meningitis, Listeria / Q000175:diagnosis; D012703:Serotyping; D013861:Thiocyanates
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D005452:Fluoresceins; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate