cDNA isolation, expression analysis, and chromosomal localization of two human zinc finger genes.
On the basis of sequence similarity in the repeated zinc finger domain, we have identified and characterized two human cDNA clones (ZNF7 and ZNF8), both encoding proteins containing potential finger-like nucleic acid binding motifs. Northern blot analysis indicates that both genes are expressed as multiple transcripts and they are ubiquitously present in many human cell lines of different embryological derivation. Moreover, their expression is modulated during in vitro induced terminal differentiation of human myeloid cell line HL-60. By in situ hybridization experiments, we have localized the ZNF7 gene to chromosome 8 (region q24) and the ZNF8 gene to the terminal band of the long arm of chromosome 20 (20q13).
Lania
L
L
Department of Genetics, University of Naples, Italy.
Donti
E
E
Pannuti
A
A
Pascucci
A
A
Pengue
G
G
Feliciello
I
I
La Mantia
G
G
Lanfrancone
L
L
Pelicci
P G
PG
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002454:Cell Differentiation; D002460:Cell Line; D002874:Chromosome Mapping; D002890:Chromosomes, Human, Pair 20; D002898:Chromosomes, Human, Pair 8; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D005796:Genes; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D008667:Metalloproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D015032:Zinc
D004268:DNA-Binding Proteins; D051741:Kruppel-Like Transcription Factors; D008667:Metalloproteins; C497248:ZNF8 protein, human; D004247:DNA; D015032:Zinc