Time-resolved polarization measurements of porphyrin fluorescence in solution and in single cells.
Porphyrin monomers, dimers and aggregates, which can be differentiated on the basis of their fluorescence lifetimes, are shown to possess different degrees of fluorescence polarization. This opens up new possibilities for microscopic imaging of these individual components in photosensitization and tumour detection. A rough estimate of the size of the porphyrin aggregates is obtained from the data of time-resolved fluorescence anisotropy.
Seidlitz
H K
HK
Gesellschaft für Strahlen- und Umweltforschung München, Institut für Angewandte Optik, Neuherberg, F.R.G.
Schneckenburger
H
H
Stettmaier
K
K
D000818:Animals; D002460:Cell Line; D004848:Epithelium / Q000032:analysis; D005454:Fluorescence Polarization / Q000379:methods; D011166:Porphyrins / Q000032:analysis*; D011524:Protoporphyrins / Q000032:analysis; D051381:Rats; D012996:Solutions; D013997:Time Factors; D014709:Vero Cells
D011166:Porphyrins; D011524:Protoporphyrins; D012996:Solutions