[Serum antibodies in dogs with autoimmune disorders recognize 40 kd glycoprotein in the nucleus of mammalian cells].
We report a new antibody specificity in 15 sera recovered from a group of dogs developing systemic lupus erythematosus (SLE) or clinically related disorders. This antibody stains in a speckled fashion the nucleus of human Hep-2 cells. Immunodiffusion tests with saline extracts of rabbit thymus showed that all 15 sera generate a common precipitation line which crosses the lines from reference sera to Sm, SS-A/ro, SS-B/La, and RNP antigens. The target nuclear antigen is a 40 kD polypeptide (p40). An important property of p40 resides in its ability to bind specifically Wheat Germ Agglutinin lectin but not Concanavalin A, supporting the notion that the antigen is a glycoprotein bearing a N-acetylglucosamine moiety.
Soulard
M
M
I.N.S.E.R.M. U no 301, Institut de Génétique moléculaire, Paris.
Barque
J P
JP
Della Valle
V
V
Danon
F
F
Larsen
C J
CJ
D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology*; D001327:Autoimmune Diseases / Q000276:immunology*; D004285:Dogs; D006023:Glycoproteins / Q000145:classification / Q000276:immunology*; D006367:HeLa Cells; D007074:Immunoglobulin G / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008322:Mammals / Q000276:immunology*; D008970:Molecular Weight
D000974:Antibodies, Antinuclear; D006023:Glycoproteins; D007074:Immunoglobulin G
Des anticorps sériques de chiens souffrant de désordres autoimmuns reconnaissent une glycoprotéine de 40 kd dans le noyau de cellules de mammifères.