Genetic transformation of intact cells of Bacillus subtilis by electroporation.
Plasmid DNAs were introduced by electroporation into Bacillus subtilis PB1424 as an alternative to competent-cell or protoplast transformation. The maximum electroporation efficiency was 10(4) transformants/microgram DNA. Parameters including growth phase of cells, ionic strength of the suspending medium, concentration and size of plasmid DNAs, amplitude and duration of the pulse, were evaluated in order to determine conditions that improved transformation efficiency.
Brigidi
P
P
Department of Pharmaceutical Sciences, University of Bologna, Italy.
De Rossi
E
E
Bertarini
M L
ML
Riccardi
G
G
Matteuzzi
D
D
D001412:Bacillus subtilis / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004560:Electricity; D005821:Genetic Techniques; D005822:Genetic Vectors; D010957:Plasmids; D014170:Transformation, Genetic
D004269:DNA, Bacterial