Identification of HTLV-I sequence in cord blood mononuclear cells of neonates born to HTLV-I antigen/antibody-positive mothers by polymerase chain reaction.
We developed a polymerase chain reaction (PCR) method which has high sensitivity and simple technique in order to investigate the presence or absence of human T lymphotropic virus type I (HTLV-I) provirus in cord blood mononuclear cells of neonates born to HTLV-I carrier mothers. Out of 40, three subjects were found to contain the HTLV-I provirus genome. These three subjects remained HTLV-I sequence-positive in follow-up study. On the other hand, when examined by a conventional technique for detection of HTLV-I-associated antigen on peripheral mononuclear cells, all 40 neonates were HTLV-I-associated antigen-negative. These results suggest that PCR is more sensitive than the conventional antigen detection method and is useful in early detection of HTLV-I infection in neonates born to HTLV-I carriers.
Saito
S
S
Department of Obstetrics and Gynecology, Nara Medical University.
Furuki
K
K
Ando
Y
Y
Tanigawa
T
T
Kakimoto
K
K
Moriyama
I
I
Ichijo
M
M
D001483:Base Sequence; D015139:Blotting, Southern; D004279:DNA, Viral / Q000032:analysis*; D005312:Fetal Blood / Q000382:microbiology; D015481:HTLV-I Antibodies / Q000032:analysis; D015490:HTLV-I Infections / Q000151:congenital / Q000635:transmission*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D006801:Humans; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000175:diagnosis* / Q000382:microbiology; D007963:Leukocytes, Mononuclear / Q000382:microbiology; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction
D004279:DNA, Viral; D015481:HTLV-I Antibodies; D015345:Oligonucleotide Probes