Efficient tumor suppression by glioma-specific murine cytotoxic T lymphocytes transfected with interferon-gamma gene.
We have recently shown that exogenous expression of the mouse interferon-gamma (IFN-gamma) gene augmented the cell-killing potential of a line of cytotoxic T lymphocytes (CTLs) specific against a murine glioma line (203-glioma). In the present work, we further investigated the in vivo antitumor effects of the E gamma-6 and E gamma-9 sublines of this CTL line transfected with the IFN-gamma gene. Using the Winn assay to test the neutralization of subcutaneous gliomas, we determined that these CTL sublines were more effective than the E-4 parent CTL line and that suppression of the tumor growth was dependent on the number of effector cells (CTLs). Moreover, intravenous injection of E gamma-9 cells was more effective in suppressing the tumor growth than intravenous injection of E-4 cells. These results suggest that transfection of antitumor effector cells with the IFN-gamma gene could improve the efficacy of adoptive immunotherapy against cancer.
Miyatake
S
S
Department of Neurosurgery, Faculty of Medicine, Kyoto University, Japan.
Nishihara
K
K
Kikuchi
H
H
Yamashita
J
J
Namba
Y
Y
Hanaoka
M
M
Watanabe
Y
Y
D000818:Animals; D003602:Cytotoxicity, Immunologic; D005910:Glioma / Q000628:therapy*; D007116:Immunization, Passive; D007167:Immunotherapy; D007371:Interferon-gamma / Q000008:administration & dosage*; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection; D014407:Tumor Cells, Cultured
D011994:Recombinant Proteins; D007371:Interferon-gamma