Crystallization and preliminary X-ray investigation of lipoxygenase 1 from soybeans.
Soybean lipoxygenase 1 has been crystallized by the vapor diffusion method in 8-10% polyethylene glycol (average Mr 3400), 0.2 M sodium acetate buffer, pH 5.2-5.6, at a protein concentration of 6-12 mg/ml. Microseeding was employed to obtain growth of large single crystals. The crystals, which diffract to at least 2.2-A spacings, are monoclinic and of space group P2(1). Cell constants are: a = 95.4, b = 94.2, and c = 50.4 A and beta = 91.4 degrees. The calculated value of Vm (2.41 A3/Da) is consistent with the probable presence of one molecule of lipoxygenase/crystallographic asymmetric unit (Z = 2).
Steczko
J
J
Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907.
Muchmore
C R
CR
Smith
J L
JL
Axelrod
B
B
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003460:Crystallization; D006863:Hydrogen-Ion Concentration; D008084:Lipoxygenase; D010944:Plants / Q000201:enzymology*; D011092:Polyethylene Glycols; D013025:Glycine max; D014961:X-Ray Diffraction
D011092:Polyethylene Glycols; D008084:Lipoxygenase