Pyrimidine ribonucleoside catabolism in Pseudomonas fluorescens biotype A.
The pyrimidine ribonucleosides uridine or cytidine were shown to serve as a source of nitrogen or carbon for the growth of Pseudomonas fluorescens strain A126. After incubation of either pyrimidine ribonucleoside with extracts of this strain, the resultant catabolic products were detected by thin-layer chromatography. It was found that pyrimidine ribonucleoside catabolism in this pseudomonad involved the enzymes nucleoside hydrolase and cytosine deaminase. The specific activities of both these enzymes could be influenced by the nitrogen or carbon source present in the medium.
Chu
C P
CP
Department of Biological Sciences, University of Southern Mississippi, Hattiesburg 39406.
West
T P
TP
D002244:Carbon / Q000378:metabolism; D002855:Chromatography, Thin Layer; D003470:Culture Media; D003596:Cytosine / Q000378:metabolism*; D043525:Cytosine Deaminase; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D009700:Nucleoside Deaminases / Q000378:metabolism; D011551:Pseudomonas fluorescens / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D014529:Uridine / Q000378:metabolism*
D003470:Culture Media; D002244:Carbon; D003596:Cytosine; D009699:N-Glycosyl Hydrolases; D009700:Nucleoside Deaminases; D043525:Cytosine Deaminase; D009584:Nitrogen; D014529:Uridine