Cytokine production in a model of wound healing: the appearance of MIP-1, MIP-2, cachectin/TNF and IL-1.
Macrophages are essential for normal wound repair and many of their effects on healing wounds are likely to be mediated by the secretion of cytokines. This study examines the appearance of messenger RNA (mRNA) for cachectin/tumor necrosis factor (TNF), IL 1, and macrophage inflammatory proteins 1 and 2 (MIP-1 and MIP-2), as well as the mature peptides, in a model of wound healing using wound chambers. RNA for all four cytokines can be detected in wound inflammatory cells by polymerase chain reaction amplification throughout the first 7 days. Cachectin/TNF and IL 1 protein levels peaked on the first day after wound chamber implantation, and MIP-1 and MIP-2 were detected only on day 3. The data suggest that these cytokines participate in the early inflammatory response to wounding.
Fahey
T J
TJ
Department of Surgery, New York Hospital-Cornell Medical Center, New York.
Sherry
B
B
Tracey
K J
KJ
van Deventer
S
S
Jones
W G
WG
Minei
J P
JP
Morgello
S
S
Shires
G T
GT
Cerami
A
A
D000818:Animals; D015153:Blotting, Western; D002465:Cell Movement; D054407:Chemokine CCL4; D054426:Chemokine CXCL2; D016207:Cytokines / Q000502:physiology*; D015870:Gene Expression; D007375:Interleukin-1 / Q000502:physiology*; D007962:Leukocytes / Q000502:physiology*; D008262:Macrophage Activation; D019402:Macrophage Inflammatory Proteins; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015846:Monokines / Q000502:physiology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*; D014945:Wound Healing
D054407:Chemokine CCL4; D054426:Chemokine CXCL2; D016207:Cytokines; D007375:Interleukin-1; D019402:Macrophage Inflammatory Proteins; D015846:Monokines; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha