A sandwich enzyme immunoassay for bovine interferon-gamma and its use for the detection of tuberculosis in cattle.
An in vitro cellular assay for bovine tuberculosis has recently been developed. This assay detects gamma-interferon released in response to specific antigen in a whole blood culture system. The bio-assay previously described for the detection of bovine gamma-interferon (IFN-gamma) has now been replaced with a sandwich enzyme immunoassay (EIA) which utilises two monoclonal antibodies to bovine IFN-gamma. The EIA detects less than 25pg/ml of recombinant bovine IFN-gamma and is specific for biologically active bovine IFN-gamma; and does not detect bovine alpha or beta interferon. IFN-gamma from sheep, goat and buffalo, but not from pig, deer or man, are also recognised by the EIA. The bovine IFN-gamma EIA when used in conjunction with the whole blood culture system has resulted in a simple, rapid and sensitive in vitro assay for specific cell mediated immune responsiveness to M. bovis infection in cattle.
Rothel
J S
JS
CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Victoria.
Jones
S L
SL
Corner
L A
LA
Cox
J C
JC
Wood
P R
PR
D000818:Animals; D000911:Antibodies, Monoclonal; D001681:Biological Assay; D002417:Cattle; D005260:Female; D007124:Immunoenzyme Techniques; D007371:Interferon-gamma / Q000032:analysis* / Q000276:immunology; D011237:Predictive Value of Tests; D011897:Random Allocation; D013045:Species Specificity; D014373:Tuberculin / Q000276:immunology; D014380:Tuberculosis, Bovine / Q000175:diagnosis*
D000911:Antibodies, Monoclonal; D014373:Tuberculin; D007371:Interferon-gamma