Human glucose-6-phosphate dehydrogenase gene carried on a yeast artificial chromosome encodes active enzyme in monkey cells.
Yeast artificial chromosomes (YACs) permit the cloning of large tracts of human DNA. A YAC containing the human glucose-6-phosphate dehydrogenase gene is shown to encode active enzyme, supporting the inference that the YAC conserves the structure of the genomic DNA.
D'Urso
M
M
International Institute of Genetics and Biophysics, Naples, Italy.
Zucchi
I
I
Ciccodicola
A
A
Palmieri
G
G
Abidi
F E
FE
Schlessinger
D
D
D000818:Animals; D015139:Blotting, Southern; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D015870:Gene Expression; D015723:Gene Library; D005796:Genes; D005822:Genetic Vectors; D015894:Genome, Human; D006150:Guanine Nucleotides / Q000235:genetics*; D006153:Guanosine Diphosphate / Q000235:genetics* / Q000378:metabolism; D000882:Haplorhini; D006801:Humans; D006822:Hybrid Cells; D015183:Restriction Mapping
D006150:Guanine Nucleotides; D006153:Guanosine Diphosphate