Mycoplasma iowae species-specific DNA probe.
A Mycoplasma iowae (MI) species-specific DNA probe (designated pMI-2) of 6.0 kbp (kilobase pairs) was isolated from an MI strain I-695 genomic library prepared in plasmid pUC8 and Escherichia coli strain JM83. When labeled with [32]P by nick translation, the probe hybridized in dot blot assays with 6 reference strains and 8 field isolates of MI but not with 16 other known species of avian mycoplasmas. The pMI-2 probe detected a minimum of 1.5 ng of MI strain I-695 chromosomal DNA. Under identical conditions of hybridization, the probe did not hybridize with a high concentration (200 ng) of M. gallisepticum or M. synoviae chromosomal DNA.
Zhao
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SH
Department of Epidemiology and Preventive Medicine, School of Veterinary Medicine, University of California, Davis 95616.
Yamamoto
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D000818:Animals; D015139:Blotting, Southern; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D009174:Mycoplasma / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D011237:Predictive Value of Tests; D013045:Species Specificity; D014422:Turkeys
D015342:DNA Probes; D004269:DNA, Bacterial