Mouse "protective protein". cDNA cloning, sequence comparison, and expression.
The "protective protein" is the glycoprotein that forms a complex with the lysosomal enzymes beta-galactosidase and neuraminidase. Its deficiency in man leads to the metabolic storage disorder galactosialidosis. The primary structure of human protective protein, deduced from its cloned cDNA, shows homology to yeast serine carboxypeptidases. We have isolated a full-length cDNA encoding murine protective protein. The nucleotide sequences as well as the predicted amino acid sequences are highly conserved between man and mouse. Domains important for the protease function are completely identical in the two proteins. Both human and mouse mature protective proteins covalently bind radiolabeled diisopropyl fluorophosphate. Transient expression of the murine cDNA in COS-1 cells yields a protective protein precursor of 54 kDa, a size characteristic of the glycosylated form. This cDNA-encoded precursor, endocytosed by human galactosialidosis fibroblasts, is processed into a 32- and a 20-kDa heterodimer and corrects beta-galactosidase and neuraminidase activities. A tissue-specific expression of protective protein mRNA is observed when total RNA from different mouse organs is analyzed on Northern blots.
Galjart
N J
NJ
Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands.
Gillemans
N
N
Meijer
D
D
d'Azzo
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002268:Carboxypeptidases / Q000235:genetics* / Q000378:metabolism; D043402:Cathepsin A; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000235:genetics*; D015870:Gene Expression; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007531:Isoflurophate / Q000378:metabolism; D008247:Lysosomes / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000172:deficiency / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014018:Tissue Distribution; D014162:Transfection; D001616:beta-Galactosidase / Q000172:deficiency / Q000378:metabolism
D006023:Glycoproteins; D012333:RNA, Messenger; D007531:Isoflurophate; D004247:DNA; D009439:Neuraminidase; D001616:beta-Galactosidase; D002268:Carboxypeptidases; C468629:CTSA protein, human; D043402:Cathepsin A