FITC-labeled lipopolysaccharide: use as a probe for liposomal membrane incorporation studies.
FITC-labeled LPS from Neisseria meningitidis can be used as a probe to follow the process of LPS incorporation into liposomal membrane and to study its interaction with a bilayer. The incorporation of FITC-LPS into the bilayer was proved by physicochemical methods as well as by liposomal LPS toxicity decrease in actinomycin D-sensitized mice. Fluorescence intensity increase was observed upon the insertion of FITC-LPS into the membrane of dehydration/rehydration vesicles and vesicles obtained by co-sonication of lipid suspension and FITC-LPS. Following FITC-LPS fluorescence polarization it was shown that the substance seems to be clusterized in the liposomal membrane starting from FITC-LPS/lipid molar ratio 1:800.
Trubetskoy
V S
VS
USSR Cardiology Research Center, Moscow.
Koshkina
N V
NV
Omel'yanenko
V G
VG
L'vov
V L
VL
Dmitriev
B A
BA
Petrov
A B
AB
Torchilin
V P
VP
D000818:Animals; D002784:Cholesterol; D003609:Dactinomycin / Q000494:pharmacology; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005454:Fluorescence Polarization; D008070:Lipopolysaccharides / Q000633:toxicity; D008081:Liposomes; D051379:Mice; D008808:Mice, Inbred CBA; D009345:Neisseria meningitidis; D010713:Phosphatidylcholines; D013010:Sonication; D013050:Spectrometry, Fluorescence; D013861:Thiocyanates
D005452:Fluoresceins; D008070:Lipopolysaccharides; D008081:Liposomes; D010713:Phosphatidylcholines; D013861:Thiocyanates; D003609:Dactinomycin; D002784:Cholesterol; D016650:Fluorescein-5-isothiocyanate