[Isolation and primary characteristics of the common species-specific outer membrane protein of Rickettsia prowazekii].
Common species-specific protein is isolated for the first time in the chromatographically pure state from the outer membrane of Rickettsia prowazekii, and its amino acid composition is determined. As revealed by chromatofocusing technique, the protein possesses pI 4.18 +/- 0.03. Three basic ninhydrin-positive compounds, differing from usual amino acids, were discovered in the protein hydrolyzate. Data suggesting the subunit structure of the isolated protein are presented.
Rodionov
A V
AV
Belousova
L S
LS
Isachkova
I P
IP
Pluzhnikova
G N
GN
Dmitriev
B A
BA
Molokova
E V
EV
D000596:Amino Acids / Q000032:analysis; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D008970:Molecular Weight; D012283:Rickettsia prowazekii / Q000032:analysis*; D013045:Species Specificity
D000596:Amino Acids; D001425:Bacterial Outer Membrane Proteins
Vydelenie i pervichnaia kharakteristika obshchevidovogo belka naruzhnoĭ membrany Rickettsia prowazekii.