DNA methylation in the Alcohol dehydrogenase-1 gene of maize.
Using a battery of methylation-sensitive restriction enzymes, cytosine methylation at 23 sites in a 7.6 kb region surrounding the Alcohol dehydrogenase-1 (Adh1) gene was measured in DNA prepared from immature maize cobs. Both the 5' upstream region and the entire coding region were hypomethylated in the two alleles examined. Methylation in Adh1 is independent of changes in Mutator transposable element methylation. The role of DNA methylation in Adh1 gene regulation is discussed.
Walbot
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V
Department of Biological Sciences, Stanford University, CA 94305-5020.
Warren
C
C
D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000483:Alleles; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D008745:Methylation; D010944:Plants / Q000235:genetics* / Q000378:metabolism; D003313:Zea mays / Q000235:genetics / Q000378:metabolism
D004247:DNA; D000426:Alcohol Dehydrogenase