title: SBD, a novel structural subunit of the Drosophila nicotinic acetylcholine receptor, shares its genomic localization with two alpha-subunits.
abstract: Nicotinic acetylcholine receptors (nAChRs) display marked heterogeneity in both vertebrates and invertebrates. Here we describe the structure of a cDNA from Drosophila melanogaster which encodes a novel nAChR beta-type subunit (SBD or beta 2). The deduced amino acid sequence of SBD displays remarkable similarity to the Drosophila alpha-subunits, ALS and SAD, while homology to the Drosophila beta-subunit ARD is less pronounced. The temporal expression of sbd transcripts during Drosophila development is similar to that of other nAChR subunit mRNAs, with high levels being found during late embryonic and late pupal stages. In embryos, sbd and als transcripts are localized in the central nervous system. The sbd gene maps cytogenetically in proximity to the als and sad genes at position 96A of chromosome 3R, suggesting the existence of a nAChR gene cluster in invertebrates.
authors.affiliation: ZMBH, Universität Heidelberg, FRG.
authors.forename: E
authors.initials: E
authors.lastname: Sawruk
authors.forename: C
authors.initials: C
authors.lastname: Udri
authors.forename: H
authors.initials: H
authors.lastname: Betz
authors.forename: B
authors.initials: B
authors.lastname: Schmitt
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002875:Chromosomes / Q000502:physiology; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000502:physiology; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D011978:Receptors, Nicotinic / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D046911:Macromolecular Substances; D015345:Oligonucleotide Probes; D011978:Receptors, Nicotinic; D004247:DNA