title: Effective use of rabbit gastric antral mucosal slices in prostaglandin synthesis and metabolism studies.
abstract: Intact slice preparations of rabbit stomach (antral mucosa, corporal mucosa, antral muscle and corporal muscle) were incubated and the released prostaglandins (PGs) were measured by reverse-phase high-performance liquid chromatography using 9-anthryldiazomethane for derivatization. With respect to total PG production, the highest amounts were generated by antral mucosal slices. Antral mucosal slices produced PGE2, 6-keto PGF1 alpha, thromboxane B2, PGF2 alpha and PGD2 (in descending order of magnitude) and possessed a high capacity for producing 13,14-dihydro-15-keto derivatives of both PGE2 and PGF2 alpha. Studies utilizing aspirin, EGTA or Ca2+ revealed that PG release by antral mucosal slices in the present in vitro system reflects a composite of the activities of phospholipase A3, PG cyclooxygenase and PG-metabolizing enzymes. These results show that antral mucosal slices will be useful in physiological and pharmacological studies on PG synthesis and metabolism of the stomach.
authors.affiliation: Department of Hygienic Chemistry, Osaka University of Pharmaceutical Sciences, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Fujimoto
authors.forename: E
authors.initials: E
authors.lastname: Nakatani
authors.forename: H
authors.initials: H
authors.lastname: Minamino
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: S
authors.initials: S
authors.lastname: Sakuma
authors.forename: T
authors.initials: T
authors.lastname: Fujita
mesh_terms: D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004533:Egtazic Acid / Q000494:pharmacology; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D009132:Muscles / Q000378:metabolism; D011453:Prostaglandins / Q000096:biosynthesis*; D011706:Pyloric Antrum / Q000378:metabolism*; D011817:Rabbits
chemical_list: D011453:Prostaglandins; D004533:Egtazic Acid; D001241:Aspirin; D002118:Calcium