title: Effects of purified anti-Lyt-2 mAb treatment on murine listeriosis: comparative roles of Lyt-2+ and L3T4+ cells in resistance to primary and secondary infection, delayed-type hypersensitivity and adoptive transfer of resistance.
abstract: Mice treated with purified anti-Lyt-2 monoclonal antibody (mAb) displayed a delayed ability to eliminate a primary Listeria monocytogenes infection from their spleens. Elimination of listeriae from the liver was unimpaired by anti-Lyt-2 mAb treatment. Treatment with anti-L3T4 mAb, alone or in combination with anti-Lyt-2 mAb, resulted in similar increases in the numbers of listeriae recovered from the spleens at 7 days after challenge. Listeria-infected mice that had been treated with anti-Lyt-2 mAb alone developed a strong delayed-type hypersensitivity (DTH) response, although it was significantly reduced as compared to control listeria-infected mice. In contrast, treatment with anti-L3T4 mAb severely impaired the development of DTH in listeria-infected mice. Treatment with anti-Lyt-2 mAb and anti-L3T4 mAb, singly or in combination, did not prevent mice from developing increased anti-listeria resistance if they were then immunized with a sublethal dose of L. monocytogenes. Treatment of mice with anti-Lyt-2 mAb or anti-L3T4 mAb before immunization, however, reduced the ability of their spleen cells to transfer anti-listeria resistance to recipient mice. These results indicate that Lyt-2+ cells make substantial contributions to the resistance of mice to primary L. monocytogenes infection, and to the ability of spleen cells from listeria-immunized mice to transfer resistance to naive recipients.
authors.affiliation: Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison.
authors.forename: C J
authors.initials: CJ
authors.lastname: Czuprynski
authors.forename: J F
authors.initials: JF
authors.lastname: Brown
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D015704:CD4 Antigens / Q000032:analysis*; D016827:CD8 Antigens; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007109:Immunity; D007116:Immunization, Passive; D007156:Immunologic Memory; D008088:Listeriosis / Q000276:immunology*; D008099:Liver / Q000276:immunology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D015704:CD4 Antigens; D016827:CD8 Antigens