Rapid purification of the peptide toxins microcystin-LR and nodularin.
An isolation procedure was developed to provide within one day microcystin-LR, a cyclic heptapeptide toxin from Microcystis aeruginosa PCC 7806. After ODS (octadecylsilyl) solid phase extraction, the crude toxin fraction was chromatographed using a strong anion exchange column. The toxin was eluted with 0.02 M ammonium bicarbonate. An at least 95% purity was revealed on HPLC separation by monitoring at 214 nm. Application of the procedure to the cyclic pentapeptide toxin nodularin from Nodularia spumigena AV2 was examined.
Martin
C
C
Institut für Biologie II, Mikrobiologie, Albert-Ludwigs-Universität, Freiburg i. Br., F.R.G.
Sivonen
K
K
Matern
U
U
Dierstein
R
R
Weckesser
J
J
D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D000458:Cyanobacteria / Q000032:analysis*; D008387:Marine Toxins / Q000302:isolation & purification*; D052998:Microcystins; D010456:Peptides, Cyclic / Q000302:isolation & purification*
D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; C063998:nodularin; C057862:cyanoginosin LR