Changes of femoral alkaline phosphatase activity in adult rats treated by sorbitol enriched or vitamin D3 deficient diet.
The effect of vitamin D3-deficiency and dietary sorbitol on serum calcium level, the activity and alkaline phosphatase (AP) pattern in femoral epiphysis were studied. Rats fed a diet supplemented with sorbitol or vitamin D3 showed the same serum calcium concentration and AP activity in serum and femur. Rats fed a vitamin D3-deficient diet displayed decreased serum calcium concentration and increased AP activity both in serum and femur. Four forms of AP were isolated from the femur of these rat groups: of Mr 100,000, 110,000, 130,000 and 165,000. Rats receiving the diet supplemented with sorbitol showed a marked rise in the activity of the Mr 165,000 form, and appearance of a new monomer of 100,000, never formed in two remaining groups.
Tardivel
S
S
Métabolisme Minéral des Mammiféres, EPHE, Physiologie, Faculté de Pharmacie, Châtenay-Malabry, France.
Banide
H
H
Dupuis
Y
Y
Ranivosoa
A
A
Fournier
P
P
Zelazowska
E
E
Porembska
Z
Z
D000469:Alkaline Phosphatase / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D002118:Calcium / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D004838:Epiphyses / Q000201:enzymology*; D005269:Femur; D008297:Male; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D013012:Sorbitol / Q000008:administration & dosage* / Q000494:pharmacology; D014808:Vitamin D Deficiency / Q000201:enzymology*
D013012:Sorbitol; D000469:Alkaline Phosphatase; D002118:Calcium