title: [A specific protein inhibiting membrane permeation of chloramphenicol in Pseudomonas aeruginosa].
abstract: A specific protein (MW 18,000) was found using chloramphenicol (CP) base-affinity chromatography of periplasmic-space proteins obtained from an impermeability-type CP-resistant Pseudomonas aeruginosa harboring plasmid kR102. Membrane reconstitution experiments using a liposome system appeared to indicate that the permeability of CP was inhibited by the specific protein.
authors.affiliation: Department of Microbiology, Tokyo College of Pharmacy.
authors.forename: K
authors.initials: K
authors.lastname: O'Hara
authors.forename: H
authors.initials: H
authors.lastname: Fukuda
authors.forename: T
authors.initials: T
authors.lastname: Kanda
authors.forename: M
authors.initials: M
authors.lastname: Kono
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification*; D002463:Cell Membrane Permeability; D002701:Chloramphenicol / Q000493:pharmacokinetics* / Q000494:pharmacology; D002846:Chromatography, Affinity; D004352:Drug Resistance, Microbial; D008970:Molecular Weight; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D002701:Chloramphenicol