Rat liver DT-diaphorase as a nitroso-reductase.
Reduction of several nitroso-compounds by purified DT-diaphorase from rat liver cytosol was investigated. Among nitroso-compounds tested, 1-nitroso-2-naphthol and p-nitrosophenol were reduced in the presence of reduced nicotinamide adenine dinucleotide phosphate (NADPH) at rates much faster than that of nitrosobenzene. On the contrary, none of the N-nitroso-compounds tested was reduced by this enzyme. Experiments on the identification of reduction products and on the inhibition with dicoumarol and the antiserum indicated that DT-diaphorase catalyzes 4-electron reduction of C-nitroso-compounds and plays a major role in the reduction of these compounds by rat liver cytosol.
Koga
N
N
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Hokama-Kuroki
Y
Y
Yoshimura
H
H
D000818:Animals; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology*; D016660:NAD(P)H Dehydrogenase (Quinone); D009603:Nitroso Compounds / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D011808:Quinone Reductases / Q000378:metabolism*; D051381:Rats
D009603:Nitroso Compounds; D010088:Oxidoreductases; D016660:NAD(P)H Dehydrogenase (Quinone); D011808:Quinone Reductases