[Detection of circulating antigen in the sera in patients with schistosomiasis using monoclonal antibody testing].
Monoclonal Antibody-Antigen Spot Test (McAb-AST) was used for detecting circulating antigen in sera from patients with schistosomiasis japonicum. McAb-AST positive rate in 308 schistosomiasis patients before treatment was 75.97%. Among the patients who had moderate or severe infection, the McAb-AST positive rates were 91.18% or 100% respectively. However, the McAb-AST cross-reactive rates in 123 normal donors, 98 clonorchiasis patients, 50 paragonimiasis patients and 56 patients with other parasitic diseases were 0%, 3.06%, 4%, and 1.79% respectively. 1.3 and 6 months after treatment, the McAb-AST negative-converting rates in patients who had negative results of stool hatching were 98.03%, 100% and 100% respectively. Compared with the results of detection of circulating antibodies, negative-conversion of circulating antigen in the patients with negative stool hatching appeared earlier and the negative-converting rate of circulating antigen was much higher. Therefore, McAb-AST had significant value in clinical practice because of its high sensitivity, specificity and good reproducibility.
Chen
Y T
YT
Schistosomiasis Research Laboratory, First Hospital, Chongqing Medical University.
Liu
Y H
YH
Wang
X G
XG
D000818:Animals; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000032:analysis*; D006801:Humans; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth