Xenograft rejection of class I-expressing transgenic skin is CD4-dependent and CD8-independent.
B10.PD1 mice are transgenic animals expressing a class I MHC antigen of pigs. B10.PD1 skin graft survival on B6 mice was prolonged by anti-CD4 antibody treatment in vivo but not by anti-CD8 treatment. These results suggest: (1) that the defect in cell-mediated recognition of xenografts involves an interaction with the xeno-MHC antigens themselves and not with the cells which express them; (2) that antigen processing and presentation of xeno-antigens on responder-type APCs is required in vivo; and (3) that CD8-independent, possibly noncytotoxic, mechanisms of xenograft rejection may exist.
Auchincloss
H
H
Department of Surgery, Massachusetts General Hospital, Boston 02114.
Moses
R
R
Conti
D
D
Sundt
T
T
Smith
C
C
Sachs
D H
DH
Winn
H J
HJ
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D015704:CD4 Antigens / Q000276:immunology*; D016827:CD8 Antigens; D006084:Graft Rejection; D006085:Graft Survival; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D008212:Lymphocyte Depletion; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D016038:Skin Transplantation / Q000276:immunology*; D013552:Swine; D013556:Swine, Miniature; D013601:T-Lymphocytes / Q000276:immunology; D013934:Thymectomy; D014183:Transplantation, Heterologous
D000945:Antigens, Differentiation, T-Lymphocyte; D015704:CD4 Antigens; D016827:CD8 Antigens; D015395:Histocompatibility Antigens Class I