title: Differential expression of low molecular weight form of Gs-alpha in neostriatum and cerebellum: correlation with expression of calmodulin-independent adenylyl cyclase.
abstract: Adenylyl cyclase of neostriatal plasma membranes is far less responsive to stimulation by Ca2+/calmodulin than is the enzyme from cerebellum. When these tissues were investigated with antibodies against known G-proteins, no difference could be detected in any G-protein species, other than Gs. Whereas the cerebellum expressed predominantly the large form of Gs-alpha, the neostriatum expressed mainly lower molecular weight forms. The possibility is considered that there is selective association between lower molecular weight forms of Gs and calmodulin-independent forms of adenylyl cyclase.
authors.affiliation: Department of Pharmacology, University of Colorado Health Sciences Center, Denver 80262.
authors.forename: D M
authors.initials: DM
authors.lastname: Cooper
authors.forename: C L
authors.initials: CL
authors.lastname: Boyajian
authors.forename: P K
authors.initials: PK
authors.lastname: Goldsmith
authors.forename: C G
authors.initials: CG
authors.lastname: Unson
authors.forename: A
authors.initials: A
authors.lastname: Spiegel
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002147:Calmodulin / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism*; D003342:Corpus Striatum / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000235:genetics*; D005786:Gene Expression Regulation; D015971:Gene Expression Regulation, Enzymologic; D015151:Immunoblotting; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D002147:Calmodulin; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases