Glycosphingolipids of porcine blood: human blood group A and H antigens with type 1 chain in erythrocytes and plasma.
Glycosphingolipids were purified from porcine erythrocytes and plasma. Two minor glycolipids with human blood group A and H antigenicities were found in both sources as components. The two antigenic glycolipids were identified as a hexaglycosylceramide (IV3 alpha GalNAc,IV2 alpha Fuc-Lc4Cer) for the A antigen and pentaglycosylceramide (IV2 alpha Fuc-Lc4Cer) for the H antigen and belonged to lactoseries (type 1 sugar chain) in contrast to those with neolacto core (type 2 sugar chain) in human erythrocytes, thereby endorsing biochemically the previous serological observations that the A antigen on porcine erythrocytes is uptake from plasma, probably the H antigen being the case. In addition to major glycolipids of globoseries in red cells and plasma, a variety of acidic glycolipids including two classes of sulphatides (sulphated galactosylceramide and sulphated lactosylceramide) and five classes of gangliosides (GM3, GD3, GM1, fucosyl GM1 and GD1a) containing N-acetylneuraminic acid and N-glycolylneuraminic acid were obtained from plasma.
Makita
A
A
Biochemistry Laboratory, Hokkaido University School of Medicine, Sapporo, Japan.
Gasa
S
S
Sako
F
F
Hanagata
G
G
D000818:Animals; D001789:Blood Group Antigens / Q000276:immunology*; D002240:Carbohydrate Sequence; D002518:Ceramides / Q000097:blood / Q000145:classification; D004912:Erythrocytes / Q000276:immunology; D005732:Gangliosides / Q000097:blood / Q000145:classification; D008401:Gas Chromatography-Mass Spectrometry; D006028:Glycosphingolipids / Q000097:blood* / Q000145:classification / Q000302:isolation & purification; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010949:Plasma / Q000276:immunology; D013433:Sulfoglycosphingolipids / Q000097:blood / Q000145:classification; D013552:Swine / Q000097:blood*
D001789:Blood Group Antigens; D002518:Ceramides; D005732:Gangliosides; D006028:Glycosphingolipids; D013433:Sulfoglycosphingolipids