Demonstration of dopamine DA-1 receptor sites in rat juxtaglomerular cells by light microscope autoradiography.
The binding of the selective DA-1 receptor antagonist [3H]-SCH 23390 in sections of rat kidney was studied using combined in vitro biochemical radio-receptor assay and autoradiography. [3H]-SCH 23390 was bound to sections of rat kidney in a manner consistent with the labeling of DA-1 receptors with a dissociation constant value of 4.2 nmol/l and a Bmax value of 180.6 fmol/mg protein. Light microscope autoradiography revealed a dense accumulation of silver grains in juxtaglomerular cells and in proximal convoluted tubule cells. These findings suggest that the stimulation of renin release elicited by dopamine and DA-1 receptor agonists may be mediated by the activation of DA-1 receptors located on juxtaglomerular cells.
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Dipartimento di Sanità Pubblica e Biologia Cellulare, Università Tor Vergata, Roma, Italy.
Ricci
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D000818:Animals; D001345:Autoradiography / Q000379:methods; D001552:Benzazepines / Q000378:metabolism; D007606:Juxtaglomerular Apparatus / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure*; D007668:Kidney / Q000033:anatomy & histology / Q000378:metabolism; D007672:Kidney Cortex / Q000378:metabolism; D007678:Kidney Glomerulus / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D007684:Kidney Tubules / Q000378:metabolism; D008297:Male; D008853:Microscopy / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D011954:Receptors, Dopamine / Q000378:metabolism*; D012834:Silver / Q000378:metabolism; D014316:Tritium
D001552:Benzazepines; D011954:Receptors, Dopamine; D014316:Tritium; D012834:Silver