title: Cell modulation of hydrophobic tailed 16S acetylcholinesterase by intracellular calcium in rat superior cervical ganglion neurons.
abstract: In primary cell cultures of rat superior cervical ganglia (SCG) the tailed asymmetric 16S molecular form of acetylcholinesterase (AChE) possesses hydrophilic (high-salt soluble, HSS) and hydrophobic (detergent extracted, DE) variants. Hydrophobic tailed acetylcholinesterase is associated with membranes through a glycolipid anchor. In the presence of tunicamycin, an antibiotic which inhibits protein glycosylation, the cellular amount of the hydrophobic DE 16S AChE is increased. Exposure of the cells to the calcium ionophore A 23187 leads to a decrease in DE 16S AChE and a correlated increase in hydrophilic HSS 16S AChE. These results suggest the existence of an endogenous processing of tailed AChE, transforming the hydrophobic variant into an hydrophilic one controlled through glycosylation and intracellular calcium.
authors.affiliation: Unité de Développement, Pathologie, Régénération du Système Neuromusculaire, INSERM U 153, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Verdière-Sahuque
authors.forename: D
authors.initials: D
authors.lastname: Goudou
authors.forename: L
authors.initials: L
authors.lastname: Garcia
authors.forename: M
authors.initials: M
authors.lastname: Nicolet
authors.forename: F
authors.initials: F
authors.lastname: Rieger
mesh_terms: D000110:Acetylcholinesterase / Q000502:physiology*; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000502:physiology*; D003902:Detergents / Q000494:pharmacology; D015536:Down-Regulation; D005260:Female; D005728:Ganglia, Sympathetic / Q000166:cytology / Q000201:enzymology*; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000201:enzymology / Q000502:physiology*; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D014415:Tunicamycin / Q000494:pharmacology
chemical_list: D003902:Detergents; D014415:Tunicamycin; D000001:Calcimycin; D000110:Acetylcholinesterase; D002118:Calcium