title: Detection of HIV-antibody evaluation of four commercially available enzyme immunoassays.
abstract: We evaluated 550 serum samples with four commercially available enzyme immunoassays and Western Blot was used as the confirmatory test for antibodies against human immunodeficiency virus (HIV). The Wellcozyme (Wellcome), Flow HIV-TEK G, and Behring test kits identified all 50 Western Blot positive samples correctly, whereas DuPont failed to detect one sample. None of the kit was able to pickup one sample that showed a faint P24 band on Western Blot strip. The frequency of false positive reaction in the 500 negative serum samples were Wellcome 0%, Behring and DuPont 0.2% and Flow HIV-TEK 0.4%.
authors.affiliation: National AIDS Centre, National Institute of Health, Islamabad.
authors.forename: F
authors.initials: F
authors.lastname: Anwer
authors.forename: N
authors.initials: N
authors.lastname: Afzal
authors.forename: S
authors.initials: S
authors.lastname: Zaidi
authors.forename: I
authors.initials: I
authors.lastname: Sultana
mesh_terms: D015492:AIDS Serodiagnosis / Q000379:methods*; D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis / Q000276:immunology*; D015153:Blotting, Western / Q000379:methods; D003951:Diagnostic Errors; D015483:HIV Antibodies / Q000032:analysis*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007194:India
chemical_list: D015483:HIV Antibodies