Characterization of the spoIVB and recN loci of Bacillus subtilis.
Two independent genes, recN and spoIVB, along with their respective promoter and termination regions, were discovered and sequenced in the 3.4-kilobase region between the ahrC and spoOA genes at map position 216 in the Bacillus subtilis chromosome map. The gene encoding a 576-amino-acid protein, which maintains a high homology with the Escherichia coli recN gene product, was adjacent to ahrC. The sequence revealed a 64,472-dalton polypeptide which contained a conserved ATP-binding site and possible lexA-type regulatory binding sequences in its promoter region. A second open reading frame identified as the spoIVB gene was directly downstream of recN. It consisted of 1,275 nucleotides which coded for a 425-amino-acid polypeptide with a molecular weight of 45,976. Phenotypic, genetic, and transcriptional analyses confirmed that this gene was spoIVB. Although no chloroform-resistant spores were produced by spoIVB-inactivated strains, under microscopic examination, phase-gray forespores were visible. The spoIVB165 mutation was localized to a 200-base-pair region in the amino-terminal portion of the polypeptide, spoIVB was not transcribed until hour 2 of sporulation in wild-type B. subtilis cells, as determined by beta-galactosidase activity assays from lacZ transcriptional fusion constructions. We found no amino acid sequence homology between the spoIVB gene product and other known bacterial proteins.
Van Hoy
B E
BE
Department of Molecular and Experimental Medicine, Research Institute of Scripps Clinic, La Jolla, California 92037.
Hoch
J A
JA
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics* / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013171:Spores, Bacterial / Q000502:physiology; D013728:Terminator Regions, Genetic; D014157:Transcription Factors; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D015345:Oligonucleotide Probes; C080950:Spo0A protein, Bacillus subtilis; D014157:Transcription Factors; D004262:DNA Restriction Enzymes; C047879:RecN protein, Bacteria