Induction of a heat shock gene (hsp70) in rabbit retinal ganglion cells detected by in situ hybridization with plastic-embedded tissue.
Elevation of body temperature by 2-3 degrees C induces a 2.7 kilobase hsp70 mRNA species in the rabbit retina within 1 hr. In situ hybridization with thin sections derived from plastic-embedded tissue permitted a higher level of resolution of retinal cell types compared to procedures which involved the use of frozen tissue sections. A prominent induction of hsp70 mRNA in retinal ganglion cells was observed when an hsp70 riboprobe was utilized for in situ hybridization. These results indicate that this neuronal cell type responds rapidly to fever-like body temperatures by inducing one of the major heat shock genes.
Masing
T E
TE
Department of Zoology, University of Toronto, Ontario, Canada.
Rush
S J
SJ
Brown
I R
IR
D000818:Animals; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000235:genetics*; D006652:Histological Techniques; D006358:Hot Temperature; D009693:Nucleic Acid Hybridization; D010969:Plastics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011817:Rabbits; D012160:Retina / Q000166:cytology; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000502:physiology*
D006360:Heat-Shock Proteins; D010969:Plastics; D012333:RNA, Messenger