Formation of deglycosylated alpha-L-fucosidase by endo-beta-N-acetylglucosaminidase in Fusarium oxysporum.
Two forms of alpha-L-fucosidase, deglycosylated and glycosylated, were found in the fucose-inducing culture broth of Fusarium oxysporum. Endo-beta-N-acetylglucosaminidase was also found in the same culture broth. The deglycosylated alpha-L-fucosidase was purified from the culture broth to homogeneity on polyacrylamide disc gel electrophoresis and analytical ultracentrifugation. Purified deglycosylated alpha-L-fucosidase was compared in chemical composition and immunological homology with glycosylated alpha-L-fucosidase which had been reported previously (K. Yamamoto, Y. Tsuji, H. Kumagai, and T. Tochikura, Agric. Biol. Chem. 50: 1689, 1986). Both enzymes had nearly the same amino acid compositions and were immunologically identical. Glycosylated alpha-L-fucosidase had mannose, galactose, and N-acetylglucosamine residues. In contrast, the deglycosylated enzyme had only N-acetylglucosamine residues. These results suggest that the deglycosylated alpha-L-fucosidase is formed by the release of sugar chains from the glycosylated form by Fusarium endo-beta-N-acetylglucosaminidase. Furthermore, various enzymatic properties were compared: the two alpha-L-fucosidases were found to exhibit similar catalytic activities and thermal stability profiles. The deglycosylated enzyme, however, was slightly unstable in the acidic pH range compared with the glycosylated enzyme.
Tsuji
Y
Y
Institute of Soysauce, Higashimaru Shoyu Co. Ltd., Hyogo, Japan.
Yamamoto
K
K
Tochikura
T
T
D000118:Acetylglucosaminidase / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D005670:Fusarium / Q000201:enzymology*; D006031:Glycosylation; D006596:Hexosaminidases / Q000378:metabolism*; D007120:Immunochemistry; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D005644:alpha-L-Fucosidase / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification
D000596:Amino Acids; D002241:Carbohydrates; D006596:Hexosaminidases; D005644:alpha-L-Fucosidase; D000118:Acetylglucosaminidase; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase