Cell-adhesive motif in region II of malarial circumsporozoite protein.
The segment of the malarial circumsporozoite (CS) protein designated Region II is highly conserved among different malarial species. A similar sequence is also present in several other proteins, including thrombospondin, properdin, and a blood-stage antigen of Plasmodium falciparum. By means of peptides synthesized from sequences of the Plasmodium vivax CS protein in the vicinity of Region II, it was found that two overlapping 18- to 20-amino acid peptides promoted the adhesion of a variety of human hematopoietic cell lines. The amino acid sequence valine-threonine-cysteineglycine (VTCG), contained within this common motif, was shown to be the critical sequence for the observed cell-adhesive properties.
Rich
K A
KA
Department of Pathology, University of Southern California School of Medicine, Los Angeles 90033.
George
F W
FW
Law
J L
JL
Martin
W J
WJ
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D002448:Cell Adhesion; D002460:Cell Line; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis; D010963:Plasmodium falciparum / Q000235:genetics*; D010966:Plasmodium vivax / Q000235:genetics*; D015800:Protozoan Proteins / Q000235:genetics*; D013861:Thiocyanates
D000953:Antigens, Protozoan; D005452:Fluoresceins; D005456:Fluorescent Dyes; D010455:Peptides; D015800:Protozoan Proteins; D013861:Thiocyanates; C041595:circumsporozoite protein, Protozoan; D016650:Fluorescein-5-isothiocyanate