title: Bacterial ferritin contains 24 haem groups.
abstract: Pseudomonas aeruginosa bacterioferritin, also known as cytochrome b1 or cytochrome b557, has been isolated with 9 haems per 24 subunits. Various forms of the protein have been prepared including the completely haem-free protein and the fully haem-loaded protein with 24 haems per 24 subunits. The presence of the core does not significantly affect haem addition or removal. The absorbance ratio of the non-haem-iron-loaded protein, 278 nm:417 nm (oxidised), can be used to estimate the haem loading.
authors.affiliation: Centre for Metalloprotein Spectroscopy and Biology, School of Chemical Sciences, University of East Anglia, Norwich, UK.
authors.forename: F H
authors.initials: FH
authors.lastname: Kadir
authors.forename: G R
authors.initials: GR
authors.lastname: Moore
mesh_terms: D001426:Bacterial Proteins; D003573:Cytochrome b Group / Q000032:analysis*; D005293:Ferritins / Q000032:analysis*; D006418:Heme / Q000032:analysis*; D007700:Kinetics; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D001426:Bacterial Proteins; D003573:Cytochrome b Group; D006418:Heme; D005293:Ferritins; C018032:bacterioferritin