title: Effect of fructose on glycogen synthesis in the perfused rat liver.
abstract: The effect of fructose on glycogen synthesis was examined in the perfused liver of starved rats. With increasing fructose concentration in the perfusate, glycogen synthesis and the % a form of glycogen synthase increased to a maximum at 2 mM and then decreased, progressively. The glucose 6-P level increased with the increase in fructose concentration. On the other hand, the ATP content was unchanged at a concentration of 2 mM or less and decreased at 3 mM or more. We also showed that the stimulation of glycogen synthesis by fructose at a concentration of 2 mM or less was due to activation of glycogen synthase by accumulated glucose 6-P and that ATP depletion at a concentration of 3 mM or more caused an increase in phosphorylase a and a decrease in glycogen synthase activity even in the presence of a high concentration of glucose 6-P.
authors.affiliation: Second Department of Surgery, Osaka University Medical School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nishi
authors.forename: Y
authors.initials: Y
authors.lastname: Kido
authors.forename: A
authors.initials: A
authors.lastname: Ogawa
authors.forename: E
authors.initials: E
authors.lastname: Furuya
authors.forename: T
authors.initials: T
authors.lastname: Mori
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005632:Fructose / Q000494:pharmacology*; D005958:Glucosephosphates / Q000378:metabolism; D006006:Glycogen Synthase / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008112:Liver Glycogen / Q000096:biosynthesis*; D008297:Male; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D014530:Uridine Diphosphate / Q000032:analysis
chemical_list: D005958:Glucosephosphates; D008112:Liver Glycogen; D005632:Fructose; D014530:Uridine Diphosphate; D000255:Adenosine Triphosphate; D006006:Glycogen Synthase