title: Protection of immunosuppressed mice against infection with pseudomonas aeruginosa by monoclonal antibodies to outer membrane protein OprI.
abstract: Two monoclonal antibodies (mAbs) designated 2A1 and 6A4 which had been shown to be directed against Pseudomonas aeruginosa outer membrane lipoprotein I were tested in cyclophosphamide treated mice for their protective ability against P. aeruginosa infection. Pretreatment of mice with either 2 mg of 2A1 or 4 mg of 6A4 in combination with lethally irradiated human leucocytes reduced the mortality after subsequent subcutaneous injection of 100 living P. aeruginosa organisms to 50% of the controls. Without leucocytes only mAb 2A1 (isotype IgG2b) showed protection (47%). Irradiated leucocytes alone without mAb did not protect the mice significantly from fatal P. aeruginosa infection.
authors.affiliation: Chirurgische Universitäts-Klinik, Freiburg, FR Germany.
authors.forename: R
authors.initials: R
authors.lastname: Rahner
authors.forename: A
authors.initials: A
authors.lastname: Eckhardt
authors.forename: M
authors.initials: M
authors.lastname: Duchêne
authors.forename: H
authors.initials: H
authors.lastname: Domdey
authors.forename: B U
authors.initials: BU
authors.lastname: von Specht
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology / Q000627:therapeutic use*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D004195:Disease Models, Animal; D007074:Immunoglobulin G / Q000008:administration & dosage / Q000276:immunology / Q000627:therapeutic use*; D007153:Immunologic Deficiency Syndromes / Q000097:blood / Q000150:complications* / Q000276:immunology; D007958:Leukocyte Count; D051379:Mice; D008807:Mice, Inbred BALB C; D011552:Pseudomonas Infections / Q000209:etiology / Q000401:mortality / Q000628:therapy*; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D015996:Survival Rate
chemical_list: D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D007074:Immunoglobulin G