Autoantibodies to mitochondrial and centromere antigens in primary biliary cirrhosis and systemic sclerosis.
High levels of IgG antibodies to the multi-enzyme complex bovine pyruvate dehydrogenase (PDHC) were detected by a sensitive ELISA in all patients with histologically proven primary biliary cirrhosis (PBC), in a minority of patients with systemic sclerosis without overt PBC or with chronic liver diseases of another cause and in no rheumatoid arthritis or normal controls. Most (15/21) but not all of these positive patients recognized a 70-kD (E2) component of PDHC on immunoblotting. Additional recognition of a centromere-associated 140-kD polypeptide was associated with the presence of scleroderma-like features (Raynaud's phenomenon and sclerodactyly) in PBC, further emphasizing the serological and clinical overlap between these two conditions.
McHugh
N J
NJ
Royal National Hospital for Rheumatic Diseases, Bath Institute for Rheumatic Diseases, England.
James
I E
IE
Fairburn
K
K
Maddison
P J
PJ
D001323:Autoantibodies / Q000276:immunology* / Q000302:isolation & purification; D002503:Centromere / Q000276:immunology*; D016009:Chi-Square Distribution; D002846:Chromatography, Affinity; D002875:Chromosomes / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000032:analysis; D008105:Liver Cirrhosis, Biliary / Q000276:immunology*; D008107:Liver Diseases / Q000276:immunology; D008928:Mitochondria / Q000276:immunology*; D011768:Pyruvate Dehydrogenase Complex / Q000276:immunology; D012594:Scleroderma, Localized / Q000276:immunology; D012595:Scleroderma, Systemic / Q000276:immunology*
D001323:Autoantibodies; D007074:Immunoglobulin G; D011768:Pyruvate Dehydrogenase Complex