Action of 6-(p-hydroxyphenylazo)-uracil on bacteriophage IG 1.
IG 1, a temperate phage of Bacillus subtilis, is strongly induced from its lysogens by 6-(p-hydroxyphenylazo)-uracil, an azopyrimidine known as selectively inhibiting the B. subtilis DNA polymerase III. IG 1 phages originated either by induction or infection multiply, abundantly, in the presence of that azopyrimidine, in spite of the drastic decline of cell viability. Chloramphenicol completely suppresses the induction effect and also blocks the formation of spontaneously induced phage particles.
Fernandes
R M
RM
Faculdade de Ciencias e Tecnologia, Universidade Nova de Lisboa, Monte de Caparica, Portugal.
de Lencastre
H
H
Archer
L J
LJ
D001412:Bacillus subtilis; D001435:Bacteriophages / Q000187:drug effects* / Q000254:growth & development; D002701:Chloramphenicol / Q000494:pharmacology; D006906:Hydroxyphenylazouracil / Q000494:pharmacology*; D014775:Virus Activation / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects
D006906:Hydroxyphenylazouracil; D002701:Chloramphenicol