Buffered charcoal-yeast extract medium for the isolation of brucellae.
A patient with chronic osteomyelitis caused by Brucella abortus had negative agglutination titers. Because of a superimposed staphylococcal infection that resulted in the overgrowth of this organism on nonselective media, brucellae were isolated only on a selective buffered charcoal-yeast extract (BCYE) agar. Sixteen strains of various Brucella species were inoculated on BCYE agar; selective BCYE agar with polymyxin, anisomycin, and cefamandole; and brucella blood agar. The growth and recovery rates on the three media tested were comparable for 14 strains. BCYE agar with polymyxin, anisomycin, and cefamandole may be useful as a selective medium for the isolation of brucellae.
Raad
I
I
Department of Medicine (Infectious Diseases), University of Florida, Gainesville.
Rand
K
K
Gaskins
D
D
D000328:Adult; D001431:Bacteriological Techniques; D002002:Brucella / Q000254:growth & development / Q000302:isolation & purification*; D002003:Brucella abortus / Q000302:isolation & purification; D002006:Brucellosis / Q000175:diagnosis; D002021:Buffers; D002606:Charcoal; D003470:Culture Media; D005069:Evaluation Studies as Topic; D006801:Humans; D008297:Male; D010019:Osteomyelitis / Q000175:diagnosis; D013045:Species Specificity; D015003:Yeasts
D002021:Buffers; D003470:Culture Media; D002606:Charcoal