title: The splenic pool of mouse stem cells: in vitro differentiation and expression of the BP-1 alloantigen on cells of the lymphoid and myeloid lineages.
abstract: The relative paucity of data about the development of the stem cell pool present in the spleen prompted this study. During in vitro cultures of B-enriched lymphocytes from mouse spleens and in the presence of a culture supernatant of WEHI-3 cells (WEHI-SUP), a population of cells expressing the BP-1 antigen appears progressively, reaches an optimal size 8 days after initiation of the culture, and disappears on day 28. In 8-day-old cultures, a minor population of cells bearing both BP-1 and B220 can be detected. The growth of this cell population, with characteristics of the B lymphoid lineage (pro-B), is strictly dependent on the presence of WEHI-SUP in the medium. After 2 weeks of culture, the BP-1 antigen is expressed on a cell population, which is essentially constituted of B220-, polynuclear cells. The BP-1 antigen, which is considered as characteristic of early cells of the B lymphoid lineage, can therefore also be expressed on cells of the myeloid lineage. The injection of BP-1+ or B220+ cells in irradiated mice can hardly reconstitute their B cell pool, whereas BP-1- and B220- cells are much more efficient in vivo progenitors of this cell lineage.
authors.affiliation: Unité d'Immunophysiologie Moléculaire, URA-145 du C.N.R.S., Institut Pasteur, Paris, France.
authors.forename: R
authors.initials: R
authors.lastname: Girard
authors.forename: L
authors.initials: L
authors.lastname: Phalente
authors.forename: R
authors.initials: R
authors.lastname: Chaby
authors.forename: T
authors.initials: T
authors.lastname: Pedron
authors.forename: G
authors.initials: G
authors.lastname: Buttin
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003470:Culture Media; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology*; D007074:Immunoglobulin G / Q000032:analysis; D007519:Isoantigens / Q000032:analysis*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens; D013154:Spleen / Q000166:cytology*
chemical_list: D000954:Antigens, Surface; C069449:BP-1 alloantigen; D003470:Culture Media; D007074:Immunoglobulin G; D007519:Isoantigens; D008934:Mitogens