Participation of PBP 3 in the acquisition of dicloxacillin resistance in Listeria monocytogenes.
Purified membranes of Listeria monocytogenes ATCC 15313 contain at least five penicillin-binding proteins. In two dicloxacillin-resistant mutants, derived from a sensitive parent strain, a 16-fold increase in the MIC of dicloxacillin was observed. A less-significant increase was detected in the MICs of other beta-lactam drugs. In the mutants, PBP 3 lost its strong affinity for dicloxacillin, but remained fully susceptible to binding of 125I-penicillin X, as compared with the wild-type strain. PBP 2 could not be detected in one of the mutants. No decrease in affinity for the radioactive tracer or dicloxacillin was detected in any other PBP of the resistant mutants.
Gutkind
G O
GO
Departamento de Microbiologia e Inmunologia, Facultad de Farmacia y Bioquimica, Buenos Aires, Argentina.
Ogueta
S B
SB
de Urtiaga
A C
AC
Mollerach
M E
ME
de Torres
R A
RA
D000217:Acyltransferases / Q000378:metabolism*; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004009:Dicloxacillin / Q000494:pharmacology*; D006602:Hexosyltransferases / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008089:Listeria monocytogenes / Q000235:genetics* / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D009097:Multienzyme Complexes / Q000378:metabolism*; D002267:Muramoylpentapeptide Carboxypeptidase / Q000378:metabolism*; D009154:Mutation; D010403:Penicillin Resistance / Q000235:genetics*; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases / Q000378:metabolism*
D001426:Bacterial Proteins; D002352:Carrier Proteins; D007457:Iodine Radioisotopes; D009097:Multienzyme Complexes; D046915:Penicillin-Binding Proteins; D004009:Dicloxacillin; D000217:Acyltransferases; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase