title: Interleukin-1 alpha inhibits the effects of gamma-interferon and tumor necrosis factor alpha on the expression of the major histocompatibility antigens by the rat endothelium.
abstract: Modulation of the major histocompatibility (MHC) antigen expression on rat endothelial cells by a mixture of cytokines has been examined. Experiments were performed employing both enzyme-linked immunoassay (ELISA) and fluorescence-activated cell-sorting (FACS) techniques and recombinant cytokines: interleukin-1 alpha (IL-1 alpha), tumor necrosis factor alpha (TNF alpha), and gamma-interferon (tau IFN). The results obtained show that TNF alpha enhances the effect of tau IFN on the expression of class I and II MHC antigens. IL-1 alpha did not affect tau IFN-induced class I expression but did inhibit tau IFN-induced class II expression. Finally, TNF alpha-induced class I MHC expression was inhibited strongly by IL-1 alpha. Pretreatment of endothelium with tau IFN did not potentiate the effects of IL-1 alpha or TNF alpha on the endothelial MHC antigen expression. These results suggest a possible anti-inflammatory role of IL-1 alpha via down-regulation of MHC antigen expression by the endothelium.
authors.affiliation: Transplantation Laboratory, University of Helsinki, Finland.
authors.forename: D
authors.initials: D
authors.lastname: Leszczynski
mesh_terms: D000818:Animals; D004730:Endothelium, Vascular / Q000187:drug effects / Q000276:immunology*; D006649:Histocompatibility Antigens / Q000276:immunology*; D007371:Interferon-gamma / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D007375:Interleukin-1 / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000494:pharmacology*
chemical_list: D006649:Histocompatibility Antigens; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma