High-frequency, site-specific recombination between lactococcal and pAM beta 1 plasmid DNAs.
In vivo recombination events involving the 75-kilobase lactose proteinase plasmid pCI301 of Lactococcus lactis subsp. lactis UC317 and the conjugative enterococcal plasmid pAM beta 1 were analyzed. A fragment, identified as containing the pCI301 recombination site, mediated greatly elevated levels of mobilization and recombination with pAM beta 1 when cloned in a nonmobilizable L. lactis-Escherichia coli shuttle vector. This latter recombination event was site and orientation specific on both plasmids. Recombination on pAM beta 1 was within the region associated with plasmid replication, but no effect on pAM beta 1 replication functions was detected. Resolution of recombinant plasmids generated derivatives indistinguishable from the parental plasmids.
Hayes
F
F
Department of Food Microbiology, University College, Cork, Ireland.
Daly
C
C
Fitzgerald
G F
GF
D003227:Conjugation, Genetic; D013294:Lactococcus lactis / Q000235:genetics*; D010957:Plasmids; D011995:Recombination, Genetic; D013291:Streptococcus / Q000235:genetics