title: Age-related alterations in follicular development and hormonal profiles in rats with 4-day estrous cycles.
abstract: Two experiments were conducted to examine the hypothesis that an alteration in follicular development is associated with advancing maternal age in the absence of prolonged estrous cycles. In Experiment 1, serum and four follicles (from one ovary per rat) were collected from young and middle-aged, 4-day cycling rats on estrus or metestrus. Number and diameter of nonatretic antral follicles greater than 200 microns in diameter were determined from serial sections of the other ovary from each rat. In Experiment 2, serum and follicles (12 +/- 2) from both ovaries were collected from young and middle-aged rats on each day of a 4-day estrous cycle. All microdissected follicles were assayed for estradiol-17 beta (E2) and all sera were assayed for E2, follicle-stimulating hormone, and luteinizing hormone by radioimmunoassay. Numbers of follicles greater than 400 microns in diameter did not differ, while numbers of follicles 200-400 microns in diameter were reduced in middle-aged rats compared to young rats (Experiment 1). The mean diameter of follicles greater than 400 microns in diameter and the follicular content of E2 was greater in middle-aged than in young rats. In Experiment 2, a greater proportion of large follicles were observed in middle-aged rats than in young rats on all days, and a greater proportion of follicles with high concentrations of E2 were observed on diestrus. We interpreted these data as indicative of an early age-related change in the control of follicular recruitment, growth, and maturation.
authors.affiliation: Department of Obstetrics and Gynecology, West Virginia University, Morgantown 26506-6302.
authors.forename: S P
authors.initials: SP
authors.lastname: Lerner
authors.forename: S
authors.initials: S
authors.lastname: Meredith
authors.forename: W V
authors.initials: WV
authors.lastname: Thayne
authors.forename: R L
authors.initials: RL
authors.lastname: Butcher
mesh_terms: D000375:Aging; D000704:Analysis of Variance; D000818:Animals; D004958:Estradiol / Q000032:analysis; D004967:Estrogens / Q000032:analysis*; D004971:Estrus; D005260:Female; D005640:Follicle Stimulating Hormone / Q000032:analysis*; D007986:Luteinizing Hormone / Q000032:analysis*; D006080:Ovarian Follicle / Q000502:physiology*; D051381:Rats
chemical_list: D004967:Estrogens; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone