Octyl glucoside extracts GTP-binding regulatory proteins from rat brain "synaptoneurosomes" as large, polydisperse structures devoid of beta gamma complexes and sensitive to disaggregation by guanine nucleotides.
GTP-binding regulatory proteins are generally purified from cholate-extracted membranes in the form of heterotrimers (G proteins) consisting of a GTP-binding subunit (alpha protein) complexed with a tightly interacted heterodimer termed beta gamma. In this study we extracted the proteins from rat brain "synaptoneurosomes" using the neutral detergent 1-octyl beta-D-glucopyranoside (octyl glucoside). Using specific antibodies for detection by immunoblotting and sucrose gradients for analyzing hydrodynamic properties, we found that each species of alpha protein (alpha subunits of stimulatory, inhibitory, and brain GTP-binding proteins) exhibited a broad range (4 S to greater than 12 S) of polydisperse structures with peak values (5 S to 7 S) considerably greater than that of heterotrimeric G proteins. The beta subunit proteins, for example, appeared as a homogeneous peak at 4.4 S within which only a fraction of the total alpha proteins can be associated. Incubation of octyl glucose extracts at 30 degrees C rapidly sedimented the alpha proteins but not the beta proteins. Incubation at 30 degrees C with guanosine 5'[gamma-thio]triphosphate (10-100 microM) prevented rapid sedimentation. Hydrodynamic analysis revealed that all alpha proteins were converted to approximately 4 S structures by the actions of guanosine 5'-[gamma-thio]triphosphate without change in the hydrodynamic properties of the beta proteins. Extraction of the membranes with sodium cholate instead of octyl glucoside resulted in complete loss of the large, polydisperse structures of the alpha proteins; the S values were approximately 4 S, in the range for beta proteins. These findings suggest that the transducing GTP-binding proteins in synaptoneurosomes exist as polydisperse, possibly multimer, structures of various size that are stable in octyl glucoside but destroyed by cholate. The polydisperse structures are not associated with beta gamma complexes and are sensitive to the disaggregating effects of guanosine 5'-[gamma-thio]triphosphate.
Nakamura
S
S
Section on Signal Transduction, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.
Rodbell
M
M
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D019826:Cholic Acid; D002793:Cholic Acids / Q000494:pharmacology; D003902:Detergents; D019204:GTP-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism; D005960:Glucosides / Q000494:pharmacology; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D046911:Macromolecular Substances; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D013574:Synaptosomes / Q000378:metabolism*; D013696:Temperature; D013873:Thionucleotides / Q000378:metabolism / Q000494:pharmacology
D002793:Cholic Acids; D003902:Detergents; D005960:Glucosides; D046911:Macromolecular Substances; D013873:Thionucleotides; C018619:octyl-beta-D-glucoside; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate; D019204:GTP-Binding Proteins; D019826:Cholic Acid