Cultivation of mammalian cells in heat-sealable pouches that are permeable to carbon dioxide.
Petri plates, 96-well plates, and other unsealed culture vessels are the chief cause of air-borne contamination of cell cultures. In this study, heat-sealable plastic pouches that are permeable to CO2 and other gases are used as a means to avoid contamination. Several applications of heat-sealable pouches are described. First, petri plates can simply be sealed in a wide variety of plastic films that are permeable to CO2. Second, a few CO2-permeable plastic films can be used directly as substrata for mammalian cell growth and can also be cut with a simple hole punch for the isolation of clones. Third, one can grow cells in chemically sterilized carbonic acid solutions and thereby avoid the use of a CO2 incubator entirely.
Klebe
R J
RJ
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284.
Lyn
S
S
Magnuson
V L
VL
Zardeneta
G
G
D000818:Animals; D002245:Carbon Dioxide; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D002999:Clone Cells; D003470:Culture Media; D006367:HeLa Cells; D006801:Humans; D008722:Methods; D010539:Permeability; D014407:Tumor Cells, Cultured
D003470:Culture Media; D002245:Carbon Dioxide