Effect of platelet-derived growth factor on the proliferation of blast progenitors from acute myeloblastic leukemia patients.
The effect of platelet-derived growth factor (PDGF) on self-renewal and terminal divisions of blast progenitors of acute myeloblastic leukemia was studied. The terminal divisions of blast progenitors were determined as the primary blast colony formation in methylcellulose culture; self-renewal was assessed by secondary colony formation by replating in methylcellulose and the recovery of clonogenic cells in suspension culture. PDGF neither enhanced nor suppressed primary or secondary blast colony formation in methylcellulose culture nor recovery of clonogenic cells in suspension. The results show that PDGF does not affect the self-renewal or terminal divisions of leukemic blast progenitors.
Nara
N
N
First Department of Internal Medicine, Tokyo Medical and Dental University.
Tohda
S
S
Nagata
K
K
Suzuki
T
T
Yamashita
Y
Y
Imai
Y
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D012016:Reference Values
D010982:Platelet-Derived Growth Factor