title: The bactericidal activity of a deuteroporphyrin-hemin mixture on gram-positive bacteria. A microbiological and spectroscopic study.
abstract: The combined antibacterial activity of various porphyrins with hemin on Gram-positive bacteria was studied. Protoporphyrin, hematoporphyrin derivative and deuteroporphyrin show only a marginal inhibitory effect in the dark. However, hemin has a strong cytotoxic effect which is independent of illumination and is equally strong in the dark. The disadvantage of hemin treatment is that it is temporary. In this study, we have demonstrated that a combination of deuteroporphyrin and hemin has a unique cytotoxic activity on Staphylococcus aureus, Streptococcus faecalis and Bacillus cereus. The effect of the combined compound is stronger than that of the separate constituents, and is as strong in the dark as in the light. Only 0.005% of the initial S. aureus population survive after a 2 h treatment. Absorption and fluorescence spectra of hemin-deuteroporphyrin mixtures in water and liposomes suggest the formation of a species with spectroscopic properties which are different from those of the two constituents.
authors.affiliation: Department of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
authors.forename: Z
authors.initials: Z
authors.lastname: Malik
authors.forename: H
authors.initials: H
authors.lastname: Ladan
authors.forename: Y
authors.initials: Y
authors.lastname: Nitzan
authors.forename: B
authors.initials: B
authors.lastname: Ehrenberg
mesh_terms: D001409:Bacillus cereus / Q000187:drug effects; D003624:Darkness; D003905:Deuteroporphyrins / Q000494:pharmacology*; D013293:Enterococcus faecalis / Q000187:drug effects; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006418:Heme / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D011166:Porphyrins / Q000494:pharmacology; D013050:Spectrometry, Fluorescence / Q000379:methods; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D003905:Deuteroporphyrins; D011166:Porphyrins; D006418:Heme; C028839:deuteroporphyrin-IX