title: High efficiency electroporation of Pseudomonas aeruginosa using frozen cell suspensions.
abstract: High efficiency transformation of Pseudomonas aeruginosa was achieved using frozen cell suspensions and high voltage electroporation. We have obtained frequencies as high as 5.8 x 10(8) transformants/micrograms of plasmid DNA using PA01 strain OT684 and a buffer of 15% glycerol-1 mM MOPS. The method allows for easy and reproducible production of frozen cell suspensions for rapid transformation of P. aeruginosa.
authors.affiliation: Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada.
authors.forename: M A
authors.initials: MA
authors.lastname: Farinha
authors.forename: A M
authors.initials: AM
authors.lastname: Kropinski
mesh_terms: D004269:DNA, Bacterial / Q000494:pharmacology; D004563:Electrochemistry; D005615:Freezing; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics*; D014169:Transformation, Bacterial
chemical_list: D004269:DNA, Bacterial