Properties of a novel nitric oxide-stimulated ADP-ribosyltransferase.
A novel ADP-ribosyltransferase is present in the cytosolic fraction of various cells. The kinetic behavior and physical properties of this enzyme's activity are clearly distinguished from other known cytosolic ADP-ribosyltransferases. Agents that release nitric oxide, such as sodium nitroprusside, greatly stimulated this activity, although this effect was dependent on the presence of intact thiol groups. Dithiothreitol, reduced glutathione, or cysteine was needed for activation of the enzyme, while N-ethylmaleimide inhibited enzyme activity. High concentrations of phosphate had a slight stimulatory effect, while high concentrations of sodium chloride and thiocyanate were inhibitory. ATP also inhibited this activity. This cytosolic ADP-ribosyltransferase is clearly distinguished from other known and characterized cytosolic transferases. Its activation by biologically active nitric oxide suggests an important role for this enzymatic activity.
Brüne
B
B
Division of Cell Biology, Burroughs Wellcome Company, Research Triangle Park, North Carolina 27709.
Lapetina
E G
EG
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D001792:Blood Platelets / Q000201:enzymology; D003545:Cysteine / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology; D004229:Dithiothreitol / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005033:Ethylmaleimide / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D006801:Humans; D009569:Nitric Oxide / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*
D009599:Nitroprusside; D000246:Adenosine Diphosphate Ribose; D009569:Nitric Oxide; D011065:Poly(ADP-ribose) Polymerases; D005978:Glutathione; D003545:Cysteine; D005033:Ethylmaleimide; D004229:Dithiothreitol