Isolation and sequencing of cDNA clones encoding ethylene-induced putative peroxidases from cucumber cotyledons.
A cDNA library from ethephon-treated cucumber cotyledons (Cucumis sativus L. cv. Poinsett 76) was constructed. Two cDNA clones encoding putative peroxidases were isolated by means of a synthetic probe based on a partial amino acid sequence of a 33 kDa cationic peroxidase that had been previously shown to be induced by ethylene. DNA sequencing indicates that the two clones were derived from two closely related RNA species that are related to published plant peroxidase sequences. Southern analysis indicates that there are 1-5 copies in a haploid genome of a gene homologous to the cDNA clones. The deduced amino acid sequences are homologous with a tobacco (55% sequence identity), a horseradish (53%), a turnip (45%), and a potato (41%) peroxidase. The cloned sequences do not encode the 33 kDa peroxidase from which the original synthetic probe was been derived, but rather other putative peroxidases. An increase in the level of mRNA is evident by 3 hours after ethephon or ethylene treatment and plateaus by 15 hours.
Morgens
P H
PH
United States Department of Agriculture, Agricultural Research Service, Kearneysville, WV 25430.
Callahan
A M
AM
Dunn
L J
LJ
Abeles
F B
FB
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D005030:Ethylenes / Q000494:pharmacology; D008969:Molecular Sequence Data; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010544:Peroxidases / Q000235:genetics*; D010944:Plants / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D005030:Ethylenes; D009943:Organophosphorus Compounds; D012333:RNA, Messenger; D004247:DNA; C036216:ethylene; D010544:Peroxidases; C005073:ethephon