Interaction of platelet-activating factor with interferon-gamma in the stimulation of interleukin-1 production by human monocytes.
Platelet-activating factor (PAF) produces both early and late inflammatory responses in vivo. The late and persistent responses to PAF may result from the production of cytokines, such as interleukin-1 (IL-1). The effects of PAF on IL-1 release by human monocytes were studied with PAF alone and in combination with interferon-gamma. These studies involved the use of D10.G4.1 cells in a proliferation assay to determine the actual concentration of active IL-1 in monocyte supernatant and pellet fractions. These studies confirmed that PAF stimulates IL-1 release at two different ranges of PAF concentrations, 100 fM to 1 pM and 100 pM to 1 nM. PAF inhibited IL-1 release at 1 or 10 pM. PAF effects on IL-1 release were specific to the form of PAF that is biologically active in most system; (S)-PAF and lyso-PAF had no effect. When monocytes were incubated with both PAF and interferon-gamma, IL-1 release was greatly stimulated at the same two ranges of PAF concentrations, 100 fM to 1 pM and 100 pM to 1 nM of PAF. PAF and interferon-gamma interacted synergistically to enhance significantly the release of IL-1.
Barthelson
R
R
Department of Medicine, University of California-San Francisco.
Valone
F
F
D000328:Adult; D015153:Blotting, Western; D003470:Culture Media; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000502:physiology*; D007375:Interleukin-1 / Q000096:biosynthesis*; D009000:Monocytes / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000031:analogs & derivatives / Q000494:pharmacology / Q000502:physiology*; D013237:Stereoisomerism
D003470:Culture Media; D007375:Interleukin-1; C029271:O-deacetyl platelet activating factor; D010972:Platelet Activating Factor; D007371:Interferon-gamma