An inhibitor of prostaglandin biosynthesis from human decidua: partial purification and properties.
An inhibitor of prostaglandin synthetase which catalyzes the conversion of arachidonic acid into prostaglandin E2 was partially purified from the 105,000 x g supernatant fraction of the human decidual cell homogenate. By means of ammonium sulfate fractionation, Mono Q ion-exchange chromatography, and gel filtration chromatography, the inhibitor was purified about 15-fold, giving a preparation with a molecular weight of 55-60 KDa. The 50% inhibitory concentration of the purified substance was approximately 0.2 mg/ml. The inhibitor may play a role in suppression of prostaglandin production by decidua in early pregnancy.
Ishihara
O
O
Department of Obstetrics and Gynaecology, Saitama Medical Centre, Saitama Medical School, Japan.
Kinoshita
K
K
Satoh
K
K
Mizuno
M
M
Shimizu
T
T
D000818:Animals; D016861:Cyclooxygenase Inhibitors; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D003656:Decidua / Q000187:drug effects / Q000378:metabolism*; D015232:Dinoprostone / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D008297:Male; D008861:Microsomes / Q000201:enzymology; D011448:Prostaglandin Antagonists / Q000302:isolation & purification* / Q000494:pharmacology; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D012669:Seminal Vesicles / Q000201:enzymology; D012756:Sheep
D016861:Cyclooxygenase Inhibitors; D011448:Prostaglandin Antagonists; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone