title: Staphylolytic enzyme from Pseudomonas aeruginosa: characterization and immunocytochemical localization.
abstract: Staphylolytic enzyme, a specific peptidase produced by Pseudomonas aeruginosa, has been characterized by using immunochemical procedures. Lytic activity was detected in the extracellular medium of Pseudomonas cultures at the beginning of the stationary growth phase. No activity was detected in bacterial cells. However, lytic protein antigen was present in periplasmic and cytoplasmic fractions, suggesting that staphylolytic enzyme is synthesized as an inactive precursor which becomes active during translocation to the extracellular broth. Results obtained in immunolocalization experiments indicate the presence of the precursor in the outer part of cells. The export pathway of staphylolytic enzyme through the periplasmic space is proposed.
authors.affiliation: Departamento de Microbiología y Biología Celular, Facultad de Biología, Universidad de La Laguna, Islas Canarias, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Carnicero
authors.forename: T B
authors.initials: TB
authors.lastname: Mansito
authors.forename: J M
authors.initials: JM
authors.lastname: Roldán
authors.forename: M A
authors.initials: MA
authors.lastname: Falcón
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D010447:Peptide Hydrolases / Q000032:analysis*; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000648:ultrastructure; D013211:Staphylococcus aureus / Q000378:metabolism*
chemical_list: D010447:Peptide Hydrolases