Recombinant porcine interferon-gamma activates in vitro porcine adherent mononuclear cells to produce interleukin 1.
The effect of recombinant porcine interferon-gamma (rPoIFN gamma) on in vitro production of interleukin 1 (IL-1) by porcine blood monocytes was studied. Three-day-old cultures of porcine adherent blood mononuclear cells were treated by doses of rPoIFN gamma for 3 or 6 days before lipopolysaccharide (LPS)-induction. While rPoIFN gamma alone had no effect, a combined treatment by rPoIFN gamma and LPS enhanced the IL-1 secretory potential of adherent mononuclear cells and, to a lesser extent, the level of cell-associated IL-1. The IL-1 activity was neutralized by anti porcine IL-1 alpha and beta antisera. These results demonstrate that rPoIFN gamma has immunomodulatory effects in vitro on porcine monokine production.
Charley
B
B
INRA, Station de Virologie et d'Immunologie Moléculaires, Jouy-en-Josas, France.
Laverne
S
S
Lavenant
L
L
D000818:Animals; D002478:Cells, Cultured; D007371:Interferon-gamma / Q000494:pharmacology*; D007375:Interleukin-1 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D009500:Neutralization Tests; D011994:Recombinant Proteins; D013552:Swine
D007375:Interleukin-1; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D007371:Interferon-gamma