Detection of immunoglobulin light-chain mRNA in lymphoid tissues using a practical in situ hybridization method.
The identification of immunoglobulin protein in routinely fixed and paraffin-embedded sections using antibodies combined with immunoperoxidase or similar techniques of detection is often problematic. We developed an in situ hybridization methodology for the identification of light-chain mRNA that is applicable to formalin-fixed, paraffin-embedded tissues, using either radiolabeled or biotinylated oligonucleotide probes based on the kappa and lambda light-chain gene-constant regions. Reactive plasma cells can be consistently identified in reactive lymphoid tissues, and a monotypic pattern of light-chain mRNA restriction was seen in each of eight cases of multiple myeloma/plasmacytoma. Immunoblasts and germinal center cells also are labeled in reactive lymphoid tissues. Using 355-labeled probes, 29 of 93 cases (30%) of non-Hodgkin's lymphomas had detectable light-chain mRNA, while 19% of non-Hodgkin's lymphomas were positive using biotinylated probes.
Weiss
L M
LM
James Irvine Center, City of Hope National Medical Center, Duarte, CA 91010.
Movahed
L A
LA
Chen
Y Y
YY
Shin
S S
SS
Stroup
R M
RM
Bui
N
N
Estess
P
P
Bindl
J M
JM
D001345:Autoradiography; D001483:Base Sequence; D005629:Frozen Sections; D005821:Genetic Techniques; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D008221:Lymphoid Tissue / Q000737:chemistry*; D008223:Lymphoma / Q000737:chemistry*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D010232:Paraffin; D012333:RNA, Messenger / Q000032:analysis*
D007147:Immunoglobulin Light Chains; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D010232:Paraffin