title: Dysfunctional C1 inhibitor Ta: deletion of Lys-251 results in acquisition of an N-glycosylation site.
abstract: Hereditary angioneurotic edema is inherited as an autosomal dominant disorder and is characterized by potentially life-threatening episodic angioedema. In type II hereditary angioneurotic edema, a dysfunctional C1 inhibitor molecule is present together with low levels of normal C1 inhibitor. About 70% of these dysfunctional proteins result from reactive center (Arg-444) mutations. We describe the deletion of nucleotides encoding Lys-251 (AAG) in C1 inhibitor Ta, the dysfunctional C1 inhibitor from a family with type II hereditary angioneurotic edema. DNA sequence analysis was derived from clones obtained through polymerase chain reaction amplification of blood monocyte C1 inhibitor mRNA. As expected, clones with both normal and abnormal sequence were isolated. The deletion was verified by protein sequence analysis. These data, together with biochemical analysis of the protein and cell-free translation studies, suggest that this deletion, by altering the normal amino acid sequence from Asn-Lys-Ile-Ser to Asn-Ile-Ser, creates a new glycosylation site. The additional carbohydrate accounts for the larger size on SDS/PAGE and very likely interferes with protein function.
authors.affiliation: Department of Pediatrics, Harvard Medical School, Boston, MA.
authors.forename: R B
authors.initials: RB
authors.lastname: Parad
authors.forename: J
authors.initials: J
authors.lastname: Kramer
authors.forename: R C
authors.initials: RC
authors.lastname: Strunk
authors.forename: F S
authors.initials: FS
authors.lastname: Rosen
authors.forename: A E
authors.initials: AE
authors.lastname: Davis
mesh_terms: D000595:Amino Acid Sequence; D000799:Angioedema / Q000235:genetics*; D001483:Base Sequence; D002474:Cell-Free System; D002872:Chromosome Deletion; D003174:Complement C1 Inactivator Proteins / Q000235:genetics* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D006801:Humans; D008239:Lysine; D008969:Molecular Sequence Data; D008970:Molecular Weight; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D003174:Complement C1 Inactivator Proteins; D012333:RNA, Messenger; C065608:complement C1 inhibitor Ta; D008239:Lysine