The blasticidin S resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome.
The blasticidin S resistance gene (bsr), originally isolated from Bacillus cereus, was studied in Bacillus subtilis. It was found that a 617 bp fragment including the intact bsr gene and its 5' flanking region could confer BS resistance on B. subtilis when integrated in its chromosome, even in a single copy state. The construction of a bsr gene cassette and its practical application as a novel selection marker for B. subtilis are reported.
Itaya
M
M
Mitsubishi Kasei Institute of Life Sciences, Tokyo.
Yamaguchi
I
I
Kobayashi
K
K
Endo
T
T
Tanaka
T
T
D000935:Antifungal Agents / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics; D005819:Genetic Markers; D009705:Nucleosides / Q000494:pharmacology; D014169:Transformation, Bacterial
D000935:Antifungal Agents; D005819:Genetic Markers; D009705:Nucleosides; C004500:blasticidin S