Overexpression of the CDC25 gene, an upstream element of the RAS/adenylyl cyclase pathway in Saccharomyces cerevisiae, allows immunological identification and characterization of its gene product.
The product of the START gene CDC25, an upstream element of the RAS/adenylyl cyclase pathway in Saccharomyces cerevisiae, was identified using specific antibodies raised against a chimeric beta-galactosidase/CDC25 protein. The CDC25 protein is poorly expressed and can be detected only when the CDC25 gene is overexpressed under the control of the galactose-inducible GAL1-10 strong promoter elements. It has a molecular weight of 180,000, is not glycosylated and is strongly associated with the particulate fraction. After deletion of residues 1255-1550 the protein is found in the soluble fraction.
Vanoni
M
M
Dipartimento di Fisologia e Biochimica Generali, Università degli Studi di Milano, Italy.
Vavassori
M
M
Frascotti
G
G
Martegani
E
E
Alberghina
L
L
D000262:Adenylyl Cyclases / Q000235:genetics*; D000906:Antibodies; D018797:Cell Cycle Proteins; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D015151:Immunoblotting; D008970:Molecular Weight; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000276:immunology; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics / Q000276:immunology; D018631:ras Proteins; D020666:ras-GRF1
D000906:Antibodies; C542351:CDC25 protein, S cerevisiae; D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D020666:ras-GRF1; D001616:beta-Galactosidase; D018631:ras Proteins; D000262:Adenylyl Cyclases