title: Transmembrane potential measurement with carbocyanine dye diS-C3-(5): fast fluorescence decay studies.
abstract: The mechanism of carbocyanine dye diS-C3-(5) fluorescence intensity variations with transmembrane potential changes has been studied using time-resolved fluorescence spectroscopy. Clear evidence is given of the transmembrane-potential-dependent partition of the dye among various sites with different fluorescence lifetimes. It was found that fluorescence decay profiles reflect the transmembrane potential changes.
authors.affiliation: Institute of Physics, Charles University, Prague, Czechoslovakia.
authors.forename: M
authors.initials: M
authors.lastname: Síp
authors.forename: P
authors.initials: P
authors.lastname: Herman
authors.forename: J
authors.initials: J
authors.lastname: Plásek
authors.forename: V
authors.initials: V
authors.lastname: Hrouda
mesh_terms: D052160:Benzothiazoles; D002138:Calibration; D002232:Carbocyanines; D004058:Diffusion; D005456:Fluorescent Dyes; D006801:Humans; D008564:Membrane Potentials; D011188:Potassium / Q000502:physiology; D011804:Quinolines; D013050:Spectrometry, Fluorescence; D013997:Time Factors; D014407:Tumor Cells, Cultured
chemical_list: D052160:Benzothiazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; D011804:Quinolines; C012944:3,3'-dipropyl-2,2'-thiadicarbocyanine; D011188:Potassium