Pertussis toxin inhibits endothelium-dependent relaxations evoked by fluoride.
Fluoride is a direct activator of G-proteins. In isolated rings of canine coronary artery, fluoride caused relaxation of rings with endothelium, but only slight contraction of rings denuded of endothelium. The endothelium-dependent relaxations to fluoride were inhibited by pertussis toxin, an inhibitor of G-proteins, or by methylene blue, an inhibitor of soluble guanylate cyclase. Therefore, fluoride causes endothelium-dependent relaxations in part by activating a pertussis toxin-sensitive G-protein in the endothelial cells.
Flavahan
N A
NA
Department of Physiology and Biophysics, Mayo Clinic, Rochester, MN 55905.
Vanhoutte
P M
PM
D000109:Acetylcholine / Q000494:pharmacology; D000535:Aluminum / Q000494:pharmacology; D000077410:Aluminum Chloride; D017607:Aluminum Compounds; D000818:Animals; D002712:Chlorides / Q000494:pharmacology; D003331:Coronary Vessels / Q000187:drug effects; D015237:Dinoprost / Q000494:pharmacology; D004285:Dogs; D005260:Female; D005459:Fluorides / Q000494:pharmacology*; D019204:GTP-Binding Proteins / Q000494:pharmacology; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D008751:Methylene Blue / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects; D009126:Muscle Relaxation / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D009569:Nitric Oxide / Q000037:antagonists & inhibitors*; D005996:Nitroglycerin / Q000494:pharmacology; D037342:Pertussis Toxin; D011433:Propranolol / Q000494:pharmacology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D017607:Aluminum Compounds; D002712:Chlorides; D010566:Virulence Factors, Bordetella; D009569:Nitric Oxide; D000077410:Aluminum Chloride; D011433:Propranolol; D015237:Dinoprost; D000535:Aluminum; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins; D005996:Nitroglycerin; D000109:Acetylcholine; D005459:Fluorides; D008751:Methylene Blue; D007213:Indomethacin