title: Comparative approach to the evaluation of hemoglobin A2 by two different methods: high-performance liquid chromatography and DE-52 microchromatography.
abstract: Hb A2 was determined in 477 subjects: 77 were affected by iron deficiency anemia, 172 were carriers of beta-thalassemia trait and 228 were normal controls. Hb A2 was determined by both DE-52 microchromatography and high-performance liquid chromatography (HPLC). The analysis of the data by linear regression demonstrated that the methods furnish overlapping results. Our findings show that HPLC is a rapid and easily reproduced method which allows a quantitative and qualitative discrimination of the various Hb fractions, making it a valid tool in screening programs for hemoglobinopathies.
authors.affiliation: Cattedra di Ematologia Pediatrica, Università di Catania, Italia.
authors.forename: P
authors.initials: P
authors.lastname: Samperi
authors.forename: R
authors.initials: R
authors.lastname: Testa
authors.forename: M
authors.initials: M
authors.lastname: Mancuso
authors.forename: G
authors.initials: G
authors.lastname: Schilirò
mesh_terms: D000328:Adult; D000747:Anemia, Hypochromic / Q000097:blood*; D002648:Child; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D006580:Genetic Carrier Screening; D006441:Hemoglobin A / Q000032:analysis*; D006443:Hemoglobin A2 / Q000032:analysis*; D006801:Humans; D008297:Male; D012016:Reference Values; D013789:Thalassemia / Q000097:blood* / Q000235:genetics
chemical_list: D006441:Hemoglobin A; D006443:Hemoglobin A2