Anti-idiotype-induced, lipopolysaccharide-specific antibody response to Pseudomonas aeruginosa.
Antibody directed to the O-specific polysaccharide (Ps) side chain of Pseudomonas aeruginosa LPS provides immunotype-specific protection against infection by virtue of enhancing opsonophagocytosis. We have developed a syngeneic anti-idiotypic antibody (mAb2) directed to a functionally active monoclonal immunotype 1 Ps-antibody (mAb1). The mAb2 performed as a molecular mimic of Ps as evidenced by 1) blocking of mAb1/mAb2 interaction by Ps, 2) blocking of mAb1/Ps binding by mAb2, 3) cross-species binding of mAb2 to human Ps antibodies from individuals immunized with the same immunotype 1 Ps, and 4) induction of anti-LPS antibody by immunization with mAb2 in syngeneic mice. Our studies thus show that an anti-idiotypic antibody may functionally mimic the O-polysaccharide of P. aeruginosa LPS, and bind to cross-reactive Id present in human Ps antibodies. We have further shown that this anti-idiotypic antibody induces anti-LPS antibody when used as an Ag in syngeneic mice, suggesting that this approach may eventually be used to successfully immunize humans.
Schreiber
J R
JR
Department of Pediatrics, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106.
Patawaran
M
M
Tosi
M
M
Lennon
J
J
Pier
G B
GB
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D001667:Binding, Competitive; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007167:Immunotherapy; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D011552:Pseudomonas Infections / Q000628:therapy; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D013045:Species Specificity
D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D007130:Immunoglobulin Idiotypes; D008070:Lipopolysaccharides