Structure and methylation patterns of the gene encoding human carbonic anhydrase I.
The gene (CAI) encoding human carbonic anhydrase I (CAI) has been isolated and shown to have a total length of 50 kb. Some 36 kb of this consists of a large intron separating the erythroid-specific promoter from the coding region. A small (54 bp) noncoding exon from within this intron is occasionally found in transcripts. Two different polyadenylation sites have been found, the most distal of which is the most commonly used. Methylation levels near the promoter differ widely in cell lines. In CAI-expressing cells, a region of DNA near the promoter is demethylated in a generally highly methylated background. Surprisingly, non-CAI-expressing cell lines show much lower levels of methylation.
Lowe
N
N
Biochemistry Department, University College London, U.K.
Brady
H J
HJ
Barlow
J H
JH
Sowden
J C
JC
Edwards
M
M
Butterworth
P H
PH
D000595:Amino Acid Sequence; D001483:Base Sequence; D002256:Carbonic Anhydrases / Q000096:biosynthesis / Q000235:genetics*; D004247:DNA / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000235:genetics; D050981:Erythroid-Specific DNA-Binding Factors; D005091:Exons; D006367:HeLa Cells; D006801:Humans; D008745:Methylation; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D015183:Restriction Mapping; D016385:TATA Box; D014157:Transcription Factors / Q000235:genetics
D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D011994:Recombinant Proteins; D014157:Transcription Factors; D004247:DNA; D002256:Carbonic Anhydrases