Effects of dietary excess amino acids on the concentrations of cholesterol, alpha-tocopherol, ascorbic acid, and copper in serum and tissues of rats.
An experiment was conducted with growing rats to investigate the effects of feeding excessive specific L-amino acids for 8 days on serum and tissue cholesterol, alpha-tocopherol, ascorbic acid, and copper, and on liver microsomal cytochrome P-450. To a 10% casein diet were added 4% L-methionine, 5% L-cystine, 5% L-histidine, 5% L-threonine, 5% L-tryptophan, 5% L-phenylalanine, 5% L-tyrosine, 6% L-valine, 7% L-isoleucine, 7% L-lysine, or 8% L-leucine. Excessive cystine and histidine increased serum cholesterol and alpha-tocopherol. Excessive cystine and methionine increased liver and kidney alpha-tocopherol and ascorbic acid. Excessive tyrosine and phenylalanine caused a marked increase in serum copper and ceruloplasmin activity, whereas excessive cystine, methionine, and histidine caused a decrease in the ceruloplasmin activity. Excessive histidine increased liver cytochrome P-450, whereas excessive tyrosine markedly decreased liver cytochrome P-450.
Katayama
T
T
Department of Applied Biochemistry, Faculty of Applied Biological Science, Hiroshima University, Higashi-Hiroshima, Japan.
Hayashi
J
J
Kishida
M
M
Kato
N
N
D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D001205:Ascorbic Acid / Q000097:blood / Q000378:metabolism*; D002570:Ceruloplasmin / Q000378:metabolism; D002784:Cholesterol / Q000097:blood / Q000378:metabolism*; D003300:Copper / Q000097:blood / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004032:Diet; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014810:Vitamin E / Q000097:blood / Q000378:metabolism*
D000596:Amino Acids; D014810:Vitamin E; D003300:Copper; D003577:Cytochrome P-450 Enzyme System; D002784:Cholesterol; D002570:Ceruloplasmin; D001205:Ascorbic Acid