Modification methylase M.Sau3239I from Streptomyces aureofaciens 3239.
By chromatography on phosphocellulose and Heparin-Sepharose the modification methylase M.Sau3239I was detected and partly purified from cells of Streptomyces aureofaciens 3239. Methylation by this enzyme protects DNA from cleavage by the restriction endonuclease R.Sau3239I. The enzyme catalyzes methylation of adenine to N-6-methyladenine in the 5'-CTCGmAG-3' recognition sequence.
Zelinková
E
E
Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Paulícek
M
M
Zelinka
J
J
D004247:DNA / Q000378:metabolism; D015257:DNA-Cytosine Methylases / Q000302:isolation & purification*; D015252:Deoxyribonucleases, Type II Site-Specific; D008745:Methylation; D009691:Nucleic Acid Denaturation; D013304:Streptomyces aureofaciens / Q000201:enzymology* / Q000235:genetics
D004247:DNA; D015257:DNA-Cytosine Methylases; C034510:CTCGAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific