title: An inhibitor of prostaglandin biosynthesis from human decidua: partial purification and properties.
abstract: An inhibitor of prostaglandin synthetase which catalyzes the conversion of arachidonic acid into prostaglandin E2 was partially purified from the 105,000 x g supernatant fraction of the human decidual cell homogenate. By means of ammonium sulfate fractionation, Mono Q ion-exchange chromatography, and gel filtration chromatography, the inhibitor was purified about 15-fold, giving a preparation with a molecular weight of 55-60 KDa. The 50% inhibitory concentration of the purified substance was approximately 0.2 mg/ml. The inhibitor may play a role in suppression of prostaglandin production by decidua in early pregnancy.
authors.affiliation: Department of Obstetrics and Gynaecology, Saitama Medical Centre, Saitama Medical School, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Ishihara
authors.forename: K
authors.initials: K
authors.lastname: Kinoshita
authors.forename: K
authors.initials: K
authors.lastname: Satoh
authors.forename: M
authors.initials: M
authors.lastname: Mizuno
authors.forename: T
authors.initials: T
authors.lastname: Shimizu
mesh_terms: D000818:Animals; D016861:Cyclooxygenase Inhibitors; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D003656:Decidua / Q000187:drug effects / Q000378:metabolism*; D015232:Dinoprostone / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D008297:Male; D008861:Microsomes / Q000201:enzymology; D011448:Prostaglandin Antagonists / Q000302:isolation & purification* / Q000494:pharmacology; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D012669:Seminal Vesicles / Q000201:enzymology; D012756:Sheep
chemical_list: D016861:Cyclooxygenase Inhibitors; D011448:Prostaglandin Antagonists; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone