Histones H1 and H4 are present near the replication fork.
The presence of histones H1 and H4 at the sites of actual DNA synthesis has been studied with Ehrlich ascites tumour cells, pulse labeled for different times with 3H-thymidine and then treated with formaldehyde to crosslink histones to DNA. The fixed chromatin fragments were sonicated to reduce the size of DNA, purified in a CsCl gradient and immunoprecipitated with antibodies to histones H1 and H4. Determination of specific radioactivity in precipitated probes showed that both histones have been associated with nascent DNA even upon 1 min pulse with 3H-thymidine, thus indicating their presence near the replication fork.
Stefanovsky
V
V
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
Dimitrov
S
S
Russanova
V
V
Pashev
I
I
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D004261:DNA Replication / Q000502:physiology*; D005557:Formaldehyde; D006657:Histones / Q000302:isolation & purification*; D009707:Nucleosomes / Q000737:chemistry*; D011233:Precipitin Tests; D013936:Thymidine
D006657:Histones; D009707:Nucleosomes; D005557:Formaldehyde; D013936:Thymidine