Thermodynamics and kinetics for the helix formation of the P3 region in Tetrahymena ribozyme.
The thermodynamic and kinetic results for the helix formation of the oligonucleotides, GACCGUCA and UGUCGGUC, which correspond to the sequence of the P3 region in Tetrahymena ribozyme are reported. The kinetic result suggested that the melting mechanism of the duplex of the oligonucleotides consisted of at least two steps because of a UU mismatch.
Sugimoto
N
N
Department of Chemistry, Faculty of Science, Konan University, Kobe, Japan.
Monden
N
N
Sasaki
M
M
Yamakage
S
S
Takaku
H
H
D000818:Animals; D001483:Base Sequence; D007700:Kinetics; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000737:chemistry / Q000378:metabolism; D016337:RNA, Catalytic / Q000737:chemistry / Q000378:metabolism*; D013768:Tetrahymena / Q000201:enzymology*; D013816:Thermodynamics
D009843:Oligoribonucleotides; D016337:RNA, Catalytic