Manifestation of diabetes mellitus on mouse follicular and pre-embryo development: effect of hyperglycemia per se.
Animal models of diabetes mellitus during pregnancy have repeatedly suggested that maternal hyperglycemia was teratogenic during organogenesis, and thus may contribute to diabetic teratogenesis. However, little attention has been focused on the effects of hyperglycemia on pre-organogenic development. In this report, we examine the effect of hyperglycemia (950 mg glucose/dL) on the development of mouse pre-embryos in vitro. B6C3F1 mice were superovulated with 5 U pregnant mare serum gonadotropin (PMSG) followed by 5 U human chorionic gonadotropin (hCG) 48 hours later. Two cell pre-embryos were recovered 48 hours later, pooled together, and randomly assigned to different treatment groups. Cultures were performed in HAM's F-10 media (Gibco, Long Island, NY) with 0.1% bovine serum albumin (BSA; Sigma, St. Louis, MO) BSA at 37 degrees C in an atmosphere of 5% CO2, 5% O2, and 90% N2 with 15 to 30 embryos per milliliter of culture fluid. Cultures were viewed daily at 24, 48, and 72 hours after culturing, with recording of the development. Compared with control pre-embryos (n = 216), embryos cultured in elevated glucose levels (950 mg/dL) (n = 226) demonstrated marked growth retardation as assessed both by (1) distribution of developmental stages at each observation point (24 hours, P less than .001; 48 hours, P less than .006; 72 hours, P less than .001); and (2) a difference in the average rank sums indicating a delay in maturation (P less than .005). In a second protocol group, pre-embryos were cultured in an equivalent amount of L-glucose; no impairment in development compared with controls was noted.(ABSTRACT TRUNCATED AT 250 WORDS)
Diamond
M P
MP
Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, CT 06510.
Harbert-Moley
K
K
Logan
J
J
Pellicer
A
A
Lavy
G
G
Vaughn
W K
WK
DeCherney
A H
AH
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D001755:Blastocyst / Q000166:cytology; D001757:Blastomeres / Q000166:cytology; D002478:Cells, Cultured / Q000187:drug effects; D004622:Embryo, Mammalian / Q000196:embryology*; D005314:Embryonic and Fetal Development; D005260:Female; D005947:Glucose / Q000494:pharmacology; D006943:Hyperglycemia / Q000150:complications / Q000196:embryology*; D051379:Mice; D010063:Ovum / Q000166:cytology; D011247:Pregnancy; D011254:Pregnancy in Diabetics / Q000196:embryology* / Q000378:metabolism; D013997:Time Factors
D001095:Arachidonic Acids; D016718:Arachidonic Acid; D005947:Glucose