title: Chromatographic and spectroscopic studies on the constituents in male and female flowers of Hagenia abyssinica.
abstract: The phenolic constituents of male and female flowers of Hagenia abyssinica were examined using chromatographic and spectroscopic techniques. Chromatographic analysis of kosins (phloroglucinols) was carried out using TLC, reversed-phase LC and glass-capillary GLC. Purification of the crude kosins was effected by silica gel column chromatography and purification of phenolic acids using ion-exchange resins and preparative TLC on silica gel. The kosins were characterized by TLC, LC, UV, IR, MS and H1 NMR spectroscopy while the phenolic acids were characterized by UV-spectroscopy, TLC and LC. The phenolic acids found were protocatechuic acid, p-hydroxybenzoic acid and vanillic acid. The content of the kosins and phenolic acids was established quantitatively for both male and female flowers.
authors.affiliation: Pharmaceutical Analysis Research Group, School of Pharmacy, University of Bradford, UK.
authors.forename: T Z
authors.initials: TZ
authors.lastname: Woldemariam
authors.forename: A F
authors.initials: AF
authors.lastname: Fell
authors.forename: P A
authors.initials: PA
authors.lastname: Linley
mesh_terms: D000351:Africa, Eastern; D002849:Chromatography, Gas; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D010636:Phenols / Q000032:analysis; D010696:Phloroglucinol / Q000031:analogs & derivatives / Q000032:analysis; D010946:Plants, Medicinal / Q000032:analysis*; D012015:Reference Standards; D012997:Solvents; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007202:Indicators and Reagents; D010636:Phenols; D012997:Solvents; C010510:protokosin; C010477:kosotoxin; C069975:alpha-kosin; D010696:Phloroglucinol