Short-term TPN containing n-3 fatty acids ameliorate lactic acidosis induced by endotoxin in guinea pigs.
We evaluated the effect of total parenteral nutrition (TPN) enriched with n-3 fatty acids on the physiologic response to endotoxin in guinea pigs. Animals were randomly assigned to receive TPN differing only in lipid source for 3.5 d. Group 1 received soybean fat emulsion (Intralipid) whereas group 2 received fish (menhaden) oil. During the last 7 h of TPN, animals were further randomized to have either saline or E coli endotoxin added to the infusate. Acid-base status and serum lactate concentrations were determined. Animals infused with soybean fat emulsions and endotoxin developed a significant metabolic acidosis, lactic acidemia, and decrease in mixed venous O2 compared with controls and fish-oil-treated animals (p less than 0.05). The significantly reduced serum lactate and higher mixed venous O2 in fish-oil-infused animals suggests that the underlying mechanism involves improvement in endotoxin-induced tissue hypoperfusion, presumably through alterations in prostaglandin metabolism.
Pomposelli
J J
JJ
Laboratory of Nutrition/Infection, Cancer Research Institute, New England Deaconess Hospital, Boston, MA 02215.
Flores
E
E
Hirschberg
Y
Y
Teo
T C
TC
Blackburn
G L
GL
Zeisel
S H
SH
Bistrian
B R
BR
D000136:Acid-Base Equilibrium / Q000187:drug effects; D000140:Acidosis, Lactic / Q000139:chemically induced* / Q000517:prevention & control; D000818:Animals; D001784:Blood Gas Analysis; D004731:Endotoxins / Q000633:toxicity*; D005217:Fat Emulsions, Intravenous / Q000008:administration & dosage; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D005395:Fish Oils / Q000494:pharmacology; D006168:Guinea Pigs; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000097:blood; D010100:Oxygen / Q000097:blood; D010289:Parenteral Nutrition, Total
D004731:Endotoxins; D005217:Fat Emulsions, Intravenous; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D007773:Lactates; D010100:Oxygen