title: Genetic mapping of the structural gene for phospholipase C of Pseudomonas aeruginosa PAO.
abstract: An insertion mutation constructed by gene replacement methods was used to map the gene corresponding to the hemolytic phospholipase C (plcS gene) in Pseudomonas aeruginosa PAO1 by R68.45-mediated conjugation. plcS mapped approximately at 67 min on the 75-min chromosomal map (B. W. Holloway, K. O'Hoy, and H. Matsumoto, p. 213-221, in S. J. O'Brien, ed., Genetic Maps 1987, vol. 4, 1987), between the markers pur-67 and pru-375 and considerably distal to the regulatory genes plcA and plcB, which are located at approximately 12 min.
authors.affiliation: Department of Clinical Bacteriology, Danderyd Hospital, Sweden.
authors.forename: V
authors.initials: V
authors.lastname: Lindgren
authors.forename: R M
authors.initials: RM
authors.lastname: Ostroff
authors.forename: M L
authors.initials: ML
authors.lastname: Vasil
authors.forename: B
authors.initials: B
authors.lastname: Wretlind
mesh_terms: D002874:Chromosome Mapping; D003227:Conjugation, Genetic; D003433:Crosses, Genetic; D005798:Genes, Bacterial; D005838:Genotype; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics*; D010738:Type C Phospholipases / Q000235:genetics*
chemical_list: D010738:Type C Phospholipases