Rapid immunoblot method for diagnosis of acute Mycoplasma pneumoniae infection.
A rapid immunoblotting technique based on the IgM response to a major immunogenic protein is described for the diagnosis of Mycoplasma pneumoniae infection. Using results of the complement fixation test as the criterion for diagnosis, the rapid immunoblot method was positive in 95.7% of patients. The sensitivity was reduced to 81.9% if the test was performed on either single sera or acute sera only from serum pairs. Although the few sera that failed to demonstrate a positive IgM response were more likely to be from older patients, there was a consistent IgM response recorded for both younger (less than 20 years) and older (greater than or equal to 20 years) patients.
Cimolai
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N
Department of Pathology, University of British Columbia, Vancouver, Canada.
Mah
D
D
Thomas
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E
Middleton
P J
PJ
D000907:Antibodies, Bacterial / Q000032:analysis; D005069:Evaluation Studies as Topic; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D007075:Immunoglobulin M / Q000032:analysis; D009177:Mycoplasma pneumoniae / Q000276:immunology; D011019:Pneumonia, Mycoplasma / Q000175:diagnosis* / Q000276:immunology; D013997:Time Factors
D000907:Antibodies, Bacterial; D007075:Immunoglobulin M