Interleukin-1: effects on rabbit ileal mucosal ion transport in vitro.
Effects of recombinant human interleukin-1 (IL-1) on electrical properties and unidirectional fluxes of Na+ and Cl- in rabbit ileum were examined in vitro employing the Ussing chamber technique. Serosal but not mucosal addition of Il-1 increased short-circuit current (Isc) in a concentration-dependent manner with a maximal effect occurring at 5 ng/ml and a half-maximal effect at 0.9 ng/ml. The time required for a maximal effect was between 30 and 40 min. Flux measurements revealed that serosal IL-1 reduced Na+ and Cl- absorption. The increase in Isc elicited by IL-1 was not altered by pretreatment of tissues with serosal atropine, tetrodotoxin, the H1-histamine receptor antagonist, mepyramine or removal of Ca2+ from the serosal bathing solution. The effects of IL-1 were inhibited by removal of Cl- from the bathing solutions, or by indomethacin or piroxicam. Levels of immunoreactive thromboxane B2 and 6-keto-PGF1 alpha and PGE2 were not significantly altered by IL-1. These results demonstrate that IL-1 can inhibit Na+ and Cl- absorption in rabbit ileum.
Chiossone
D C
DC
Department of Drug Delivery, Smith Kline and French Laboratories, King of Prussia, PA 19406-0939.
Simon
P L
PL
Smith
P L
PL
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000502:physiology; D002712:Chlorides / Q000378:metabolism; D004594:Electrophysiology; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology*; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D011453:Prostaglandins / Q000378:metabolism; D011738:Pyrilamine / Q000494:pharmacology; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism; D013779:Tetrodotoxin / Q000494:pharmacology
D002712:Chlorides; D007375:Interleukin-1; D008070:Lipopolysaccharides; D011453:Prostaglandins; D011994:Recombinant Proteins; D000001:Calcimycin; D013779:Tetrodotoxin; D001285:Atropine; D012964:Sodium; D011738:Pyrilamine; D002118:Calcium; D007213:Indomethacin