Development of a beta-galactosidase alpha-complementation system for molecular cloning in Bacillus subtilis.
A versatile beta-galactosidase alpha-complementation system for Bacillus subtilis was developed, which can be used for molecular cloning in this Gram+ organism. The cloning system, which is based on the highly efficient host-vector system 6GM-pHP13, offers several advantages over previously described systems: (1) convenient direct selection of recombinants; (2) the cloning of large heterologous DNA fragments with high efficiency; and (3) the availability of six unique target sites: SphI, NdeI, NheI, BamHI, SmaI and EcoRI.
Haima
P
P
Department of Microbiology, Center of Biological Sciences, Haren, The Netherlands.
van Sinderen
D
D
Schotting
H
H
Bron
S
S
Venema
G
G
D001412:Bacillus subtilis / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D005696:Galactosidases / Q000235:genetics*; D015870:Gene Expression; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D001616:beta-Galactosidase / Q000235:genetics*
D005696:Galactosidases; D001616:beta-Galactosidase