Detection of PR-b proteins in tobacco roots infected with Chalara elegans.
All previous studies on pathogenesis-related (b) protein (PR-b) induction in tobacco have been carried out on leaves or callus tissue. This paper reports the production of PR-b proteins also in roots of tobacco plants (Nicotiana tabacum cv. Xanthi NC) infected with Chalara elegans. Antiserum against PR-b1 reacted with PR-b1, PR-b2 and PR-b3 and gave the same pattern of reaction as for leaves. Antiserum against PR-b5 revealed the presence of PR-b4, PR-b5 and, very weakly, PR-b6 which have been shown to be beta-1,3 glucanases. Antiserum against PR-b7 reacted with both PR-b7 and PR-b8 which are chitinases.
Tahiri-Alaoui
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A
Laboratoire de Phytoparasitologie INRA-CNRS, INRA, Dijon, France.
Dumas
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E
Gianinazzi
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S
D007120:Immunochemistry; D003904:Mitosporic Fungi / Q000472:pathogenicity; D010940:Plant Proteins / Q000096:biosynthesis* / Q000276:immunology; D010944:Plants / Q000378:metabolism* / Q000382:microbiology; D010947:Plants, Toxic; D014026:Nicotiana / Q000378:metabolism / Q000382:microbiology
D010940:Plant Proteins; C053376:pathogenesis-related proteins, plant