FIA-fluorimetric determination of thiamine.
A flow injection-fluorimetric determination of thiamine is reported. The procedure is based on the oxidation of the analyte with potassium hexacyanoferrate(III) immobilized on an anionic exchange resin; the fluorescence is monitored in aqueous basic solution. Concentrations of the vitamin of 0.1-4 ppm have been determined; the relative standard deviation was 1.8%. The injection rate was 28 samples/h. The influence of other substances and the determination of the drug in a pharmaceutical formulation are also reported.
Martinez Calatayud
J
J
Departamento de Quimica Analitica, Universidad de Valencia, Spain.
Gomez Benito
C
C
gaspar Gimenez
D
D
D005470:Fluorometry; D007202:Indicators and Reagents; D010084:Oxidation-Reduction; D012116:Resins, Plant; D013831:Thiamine / Q000032:analysis*
D007202:Indicators and Reagents; D012116:Resins, Plant; D013831:Thiamine