Transfer of hygromycin resistance into Brassica napus using total DNA of a transgenic B. nigra line.
The successful transfer of a marker gene (hpt gene) from Brassica nigra into B. napus via direct gene transfer was demonstrated. Total DNA was isolated from a hygromycin-resistant callus line, which contained three to five copies of the hpt gene. This line had been produced via direct gene transfer with the hygromycin resistance-conferring plasmid pGL2. The treatment of B. napus protoplasts with genomic DNA of B. nigra (HygR) resulted in relative transformation frequencies of 0.1-0.4%. Similar transformation rates were obtained in direct gene transfer experiments using B. napus protoplasts and plasmid pGL2.
Golz
C
C
Institut für Angewandte Genetik, FU Berlin, FRG.
Köhler
F
F
Schieder
O
O
D001937:Brassica / Q000201:enzymology / Q000235:genetics*; D002934:Cinnamates; D004247:DNA / Q000235:genetics; D004351:Drug Resistance; D017343:Genes, Plant; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D007527:Isoenzymes / Q000032:analysis; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D010957:Plasmids; D011523:Protoplasts / Q000187:drug effects; D013045:Species Specificity; D014170:Transformation, Genetic
D002934:Cinnamates; D007527:Isoenzymes; D010940:Plant Proteins; D006921:Hygromycin B; C026273:hygromycin A; D004247:DNA