Analysis of cytological changes in hepatocytes from rats dosed with 3'-methyl-4-dimethylaminoazobenzene: initial response appears to involve cytokinesis of binucleated cells.
The reduction in the ratio of tetraploid (4N + 2 X 2N) to diploid (2N) hepatocytes in the adult rat after treatment with the hepatocarcinogen 3'-methyl-4-dimethylaminoazobenzene (3'M) has been investigated. Analysis of isolated hepatocytes 18-28 days after treatment has confirmed that initially some of the 2 X 2N hepatocytes are converted into 2N cells by cytokinesis, and that there is no DNA synthesis during this process. Shortly afterwards nonpolyploidizing growth commences by proliferation of some 2N cells.
Kelly
M D
MD
ICI Central Toxicology Laboratories, Alderley Park, Macclesfield, Cheshire, U.K.
Styles
J A
JA
Pritchard
N R
NR
D000284:Administration, Oral; D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000502:physiology; D007399:Interphase / Q000187:drug effects; D008099:Liver / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D008297:Male; D008749:Methyldimethylaminoazobenzene / Q000008:administration & dosage / Q000494:pharmacology*; D011003:Ploidies; D051381:Rats; D004124:p-Dimethylaminoazobenzene / Q000494:pharmacology*
D008749:Methyldimethylaminoazobenzene; D004124:p-Dimethylaminoazobenzene