Expression of a rice glutelin promoter in transgenic tobacco.
A chimeric gene consisting of the 5' flanking sequences of a rice glutelin gene (Gt3) linked to the chloramphenicol acetyltransferase (CAT) coding segment was introduced into tobacco via Agrobacterium tumefaciens-mediated transformation. CAT enzyme activity could be detected in extracts from seeds as early as 8 days after flowering and obtained a maximum level at 16 days after flowering, the onset of overall protein accumulation. Significant expression of CAT activity in non-seed tissues occurred in some, but not all plants, suggesting possible chromosome position effects on non-seed tissue expression. A positive correlation was observed between expression levels in seeds and gene copy numbers.
Leisy
D J
DJ
Institute of Biological Chemistry, Washington State University, Pullman 99164-6340.
Hnilo
J
J
Zhao
Y
Y
Okita
T W
TW
D002678:Chimera; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D017343:Genes, Plant; D005983:Glutens / Q000235:genetics*; D007700:Kinetics; D012275:Oryza / Q000235:genetics*; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D012231:Rhizobium / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics*; D014170:Transformation, Genetic
D005983:Glutens; D015500:Chloramphenicol O-Acetyltransferase