title: Contribution of immunohistochemistry toward the diagnosis of tumors of laboratory rats.
abstract: A polyclonal antibody detecting S-100 protein (S-100) and a monoclonal antibody demonstrating epithelial cell (Lu-5) were used in addition to routine, hematoxylin-eosin stain to improve the identification of tumors of neural or epithelial origin diagnosed in conventionally treated tissue from rat bioassays. Among 108 lesions tested for S-100, 51 reacted positively; they included benign and malignant schwannoma, endomyocardial disease, some cases of benign and malignant thymoma, and renal tubular adenoma. S-100 protein is considered particularly useful for discriminating of neoplasms of Schwann cell origin from mesenchymal tumors. 31 of 51 lesions tested for Lu-5 reacted positively: they comprised adenoma, carcinoma, benign and malignant thymoma and atriocaval node tumor of the heart. Lu-5 was especially useful to distinguish epithelial from mesenchymal neoplasms and was capable of identifying epithelial elements in lymphocyte-rich thymomas as well as in dedifferentiated or autolytic tumors. The binding of both antibodies in neoplastic tissue was compared with a complete set of anatomically normal rat tissues.
authors.affiliation: Ciba-Geigy AG, Basel, Switzerland.
authors.forename: C
authors.initials: C
authors.lastname: Landes
authors.forename: K
authors.initials: K
authors.lastname: Heider
authors.forename: A L
authors.initials: AL
authors.lastname: Krinke
authors.forename: G J
authors.initials: GJ
authors.lastname: Krinke
authors.forename: A T
authors.initials: AT
authors.lastname: Mahrous
authors.forename: R
authors.initials: R
authors.lastname: Hess
mesh_terms: D000818:Animals; D000906:Antibodies; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002277:Carcinoma / Q000175:diagnosis; D003937:Diagnosis, Differential; D005260:Female; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000175:diagnosis; D008637:Mesenchymoma / Q000175:diagnosis; D009374:Neoplasms, Experimental / Q000175:diagnosis*; D051381:Rats; D011919:Rats, Inbred Strains; D009418:S100 Proteins / Q000378:metabolism*
chemical_list: D000906:Antibodies; D002135:Calcium-Binding Proteins; D009418:S100 Proteins