title: Purification of pertussis toxin substrates from bovine heart membranes.
abstract: Pertussis toxin substrates were purified from bovine heart membranes by means of a sequential column chromatographic techniques. The elution pattern from the final ion-exchange chromatography and relative mobilities of these peptides on SDS-polyacrylamide gel electrophoresis revealed that the bovine heart membranes contained two distinct pertussis toxin substrates: Gi2 and Go.
authors.affiliation: Department of Cardiovascular Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Urasawa
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Murakami
authors.forename: H
authors.initials: H
authors.lastname: Kawaguchi
authors.forename: H
authors.initials: H
authors.lastname: Yasuda
mesh_terms: D000067956:Adenylyl Cyclase Inhibitors; D000818:Animals; D002417:Cattle; D019204:GTP-Binding Proteins / Q000235:genetics / Q000302:isolation & purification*; D008565:Membrane Proteins / Q000302:isolation & purification*; D008970:Molecular Weight; D009206:Myocardium / Q000737:chemistry*; D037342:Pertussis Toxin; D010566:Virulence Factors, Bordetella / Q000378:metabolism*
chemical_list: D000067956:Adenylyl Cyclase Inhibitors; D008565:Membrane Proteins; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins