[The effect of ts-mutation on expression of genes induced by heat shock in Drosophila melanogaster. III. Synthesis of proteins cognate to HSP70].
2D-electrophoresis performed as described by O'-Farrel has revealed clear-cut differences in the pattern of proteins synthesized in the cells of wild-type flies and in the cells of ts-lethal. The cells of the mutant studied after heat-shock exhibit not only the lack of HSP83 and HSP35 but also the absence of a few of the heat-shock proteins belonging to the HSP70 group. Moreover, in the cells of the mutant an intensive synthesis of a protein with mol. weight 72 KDa was observed after heat-shock. This protein belongs to highly abundant heat-shock cognate proteins (HSCP) which are usually not induced by temperature elevation.
Evgen'ev
M B
MB
Denisenko
O N
ON
D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics*; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D006358:Hot Temperature; D008970:Molecular Weight; D009154:Mutation
D006360:Heat-Shock Proteins
Vliianie ts-mutatsii na ékspressiiu genov, indutsiruemykh teplovym shokom u Drosophila melanogaster. Soobshchenie III. Sintez belkov, rodstvennykh BShT70.