Specific, high-affinity binding sites for human luteinizing hormone (hLH) and human chorionic gonadotrophin (hCG) in Candida species.
The presence of specific binding sites for [125I]-labelled hLH and hCG is described in Candida species. Binding was present in three strains of Candida albicans, and in Candida tropicalis, and was greatest in microsomes, though binding was also present in cytosol fractions. hLH and hCG mutually competed for these binding sites. Other hormones did not bind and did not compete for hLH binding sites. Scatchard plots showed two classes of binding sites, one with high affinity, low capacity and the other with lower affinity, high capacity binding in both microsomes and cytosol. This is the first report of specific binding sites for mammalian peptide hormones in a yeast.
Bramley
T A
TA
University of Edinburgh, Department of Obstetrics and Gynaecology, Scotland, U.K.
Menzies
G S
GS
Williams
R J
RJ
Adams
D J
DJ
Kinsman
O S
OS
D000818:Animals; D001665:Binding Sites; D002175:Candida / Q000378:metabolism*; D002176:Candida albicans / Q000378:metabolism; D006063:Chorionic Gonadotropin / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000378:metabolism; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D013006:Growth Hormone / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D007986:Luteinizing Hormone / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism; D011817:Rabbits; D012756:Sheep
D006063:Chorionic Gonadotropin; D004815:Epidermal Growth Factor; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013006:Growth Hormone