Immunoturbidimetric quantification of serum immunoglobulin G concentration in foals.
Immunoturbidimetric determination of serum IgG concentration in foals was compared with the reference methods of single radial immunodiffusion and serum protein electrophoresis. High positive correlations were discovered when the technique was compared with either of these reference methods. The zinc sulfate turbidity test for serum IgG estimation was also evaluated. Although a positive correlation was discovered when the latter method was compared with reference methods, it was not as strong as the correlation between reference methods and the immunoturbidimetric method. The immunoturbidimetric method used in this study is specific and precise for equine serum IgG determination. It is rapid and, thus, is advantageous when timely evaluation of critically ill foals is necessary. The technique should be adaptable to various spectrophotometers and microcomputers for widespread application in veterinary medicine.
Bauer
J E
JE
Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville 32610.
Brooks
T P
TP
D000818:Animals; D001798:Blood Proteins / Q000032:analysis; D004586:Electrophoresis / Q000662:veterinary; D006736:Horses / Q000097:blood*; D005779:Immunodiffusion / Q000662:veterinary; D007074:Immunoglobulin G / Q000032:analysis*; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D012015:Reference Standards
D001798:Blood Proteins; D007074:Immunoglobulin G