title: Glycosidase activities of mycoplasmas.
abstract: The activities of alpha- and beta-glucosidase, beta-galactosidase and beta-N acetylglucosaminidase were assessed at acidic pH by fluorimetry using the appropriate 4-methylumbelliferyl substrate in four Mycoplasma species (M. pneumoniae, M. gallisepticum, M. hominis and M. capricolum) and in Acholeplasma laidlawii. The glycosidase activities were in a low range (0.1-4.2 nmole per h per mg protein) with the exception of higher activities of beta-N-acetylglucosaminidase in A. laidlawii. The enzyme levels of a virulent and a nonvirulent strain of M. pneumoniae were comparable. Despite the very sensitive assay, neuraminidase activity was not detected in M. pneumoniae and M. gallisepticum. No induction of alpha-glucosidase could be demonstrated for M. pneumoniae or A. laidlawii. At least part of the glycosidase activities was localized in the membrane fraction of all mycoplasmas studied. This may support the hypothesis that pathogenic mycoplasmas, being membrane parasites, may modify, by their glycosidases, some host cell glycoconjugates. However, our study did not distinguish the pathogenic mycoplasmas to possess a characteristic glycosidase profile.
authors.affiliation: Department of Membrane and Ultrastructure Research, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
authors.forename: I
authors.initials: I
authors.lastname: Kahane
authors.forename: A
authors.initials: A
authors.lastname: Reisch-Saada
authors.forename: M
authors.initials: M
authors.lastname: Almagor
authors.forename: P
authors.initials: P
authors.lastname: Abeliuck
authors.forename: S
authors.initials: S
authors.lastname: Yatziv
mesh_terms: D000118:Acetylglucosaminidase / Q000032:analysis; D000128:Acholeplasma laidlawii / Q000201:enzymology; D005470:Fluorometry; D006026:Glycoside Hydrolases / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D009174:Mycoplasma / Q000201:enzymology* / Q000472:pathogenicity; D009177:Mycoplasma pneumoniae / Q000201:enzymology / Q000472:pathogenicity; D014774:Virulence; D000520:alpha-Glucosidases / Q000032:analysis; D001616:beta-Galactosidase / Q000032:analysis; D001617:beta-Glucosidase / Q000032:analysis
chemical_list: D006026:Glycoside Hydrolases; D000520:alpha-Glucosidases; D001617:beta-Glucosidase; D001616:beta-Galactosidase; D000118:Acetylglucosaminidase