title: Diode array detection of low level co-eluting species in high-performance liquid chromatography.
abstract: A simple and sensitive method for detecting low levels (0.5 and 1.0%, w/w) of co-eluting species in HPLC has been developed. This method is based on the subtraction of normalized peak up-slope and down-slope spectra from that of the apex. Visual inspection of the resultant "difference spectra" allows for a qualitative judgement regarding the integrity of the peak under consideration.
authors.affiliation: Sterling Research Group, Alnwick, Northumberland, UK.
authors.forename: N H
authors.initials: NH
authors.lastname: Anderson
authors.forename: M R
authors.initials: MR
authors.lastname: Gray
authors.forename: C J
authors.initials: CJ
authors.lastname: Hinds
mesh_terms: D000865:Antazoline / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003613:Danazol / Q000032:analysis; D005938:Glucocorticoids / Q000032:analysis; D007202:Indicators and Reagents; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005938:Glucocorticoids; D007202:Indicators and Reagents; D000865:Antazoline; D003613:Danazol