title: [Determination of sulbactam in human serum using capillary isotachophoresis].
abstract: A method of determination of sulbaktam in human serum by capillary isotachophoresis with the use of a conductivity detector was worked out. Prior to the proper analysis, a pretreatment of the sample of serum was carried out by extracting sulbaktam to butyl acetate. The total yield of the proposed analytical procedure with an extraction first stage approaches 94%. The smallest determinable amount of the sample corresponds to 1 micrograms of sulbaktam in 1 ml of serum. The reported method was employed to evaluate the samples of sera of volunteers after intravenous administration of the dosage form sulbaktam-ampicillin (VUFB) and the foreign pharmaceutical preparation Unasyn (Pfizer). Statistically insignificant differences in pharmacokinetic parameters have confirmed that the preparations are highly bio-equivalent.
authors.affiliation: Výzkumný ústav pro farmacii a biochemii, Praha.
authors.forename: I
authors.initials: I
authors.lastname: Jelínek
authors.forename: H
authors.initials: H
authors.lastname: Krejcírová
authors.forename: J
authors.initials: J
authors.lastname: Dohnal
authors.forename: Z
authors.initials: Z
authors.lastname: Roubal
authors.forename: V
authors.initials: V
authors.lastname: Holá
authors.forename: V
authors.initials: V
authors.lastname: Rejholec
mesh_terms: D004586:Electrophoresis / Q000379:methods*; D006801:Humans; D013407:Sulbactam / Q000097:blood*
chemical_list: D013407:Sulbactam
vernacular_title: Stanovení sulbaktamu v lidském séru kapilární izotachoforézou.