title: Oligonucleotides with pyrene fluorophore at the sugar fragment: synthesis and properties in binding to complementary polynucleotide.
abstract: Oligonucleotides with 1-pyrenylmethyl substituent at the designated sugar residue were synthesized by using 5'-dimethoxytrityl 2'-(1-pyrenylmethyl)uridine 3'-phosphorobisdiethylamidite 1. It was shown that the pyrene-oligonucleotides have enhanced affinity in binding to the complementary polynucleotide sequence. The fluorescence yield and life time of the pyrene-oligonucleotide was drastically enhanced when bound by the complementary polynucleotide.
authors.affiliation: Department of Applied Chemistry, Himeji Institute of Technology, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yamana
authors.forename: T
authors.initials: T
authors.lastname: Gokota
authors.forename: Y
authors.initials: Y
authors.lastname: Ohashi
authors.forename: H
authors.initials: H
authors.lastname: Ozaki
authors.forename: M
authors.initials: M
authors.lastname: Kitamura
authors.forename: H
authors.initials: H
authors.lastname: Nakano
authors.forename: O
authors.initials: O
authors.lastname: Sangen
authors.forename: T
authors.initials: T
authors.lastname: Shimidzu
mesh_terms: D001483:Base Sequence; D002241:Carbohydrates / Q000737:chemistry; D005453:Fluorescence; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D011721:Pyrenes / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; D009841:Oligonucleotides; D011721:Pyrenes