A high-performance liquid chromatography method for determination of 4-aminopyridine in tissues and urine.
An analytical method was developed to measure 4-aminopyridine in tissues and urine to determine appropriate diagnostic samples in acute poisoning cases. Tissues from rats dosed with 4-aminopyridine were extracted with methylene chloride. Extracts were analyzed by high-performance liquid chromatography using an isocratic solvent system of acetonitrile and aqueous solution (15/85 v/v) consisting of 0.015 M sodium salt of l-heptane-sulfonic acid, 0.002 M tetramethylammonium bromide, and 0.01 M sodium dihydrogen phosphate adjusted to pH 3.0 with phosphoric acid. We concluded that suitable diagnostic samples for acute poisoning cases include stomach contents, kidney, liver, and urine.
Casteel
S W
SW
Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine, University of Missouri-Columbia 65205.
Thomas
B R
BR
D015761:4-Aminopyridine / Q000032:analysis* / Q000506:poisoning / Q000652:urine; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005766:Gastrointestinal Contents / Q000737:chemistry*; D007668:Kidney / Q000737:chemistry*; D008099:Liver / Q000737:chemistry*; D008297:Male; D011041:Poisoning / Q000175:diagnosis / Q000662:veterinary; D051381:Rats; D011919:Rats, Inbred Strains
D015761:4-Aminopyridine