Down-regulation of phytochrome mRNA abundance by red light and benzyladenine in etiolated cucumber cotyledons.
Northern blot analysis revealed that a single 4.2 kb phytochrome mRNA species was detectable in cotyledons excised from five-day-old etiolated cucumber seedlings. Intact etiolated five-day-old cucumber seedlings were given a red light or benzyladenine treatment, and cotyledons were harvested at various times following treatment. The abundance of phytochrome mRNA in the cotyledons was quantitated using 32P-labeled RNA probes and slot blot analysis. By 2 h after irradiation the phytochrome mRNA level was reduced to 40% of the initial abundance and reaccumulation began by 3 h after irradiation. Reaccumulation of phytochrome mRNA to the time-zero dark control level was achieved by 10 h after treatment. A decrease in phytochrome mRNA abundance was evident by 2 h after benzyladenine treatment, and a maximal reduction to 45% of the time-zero dark control was attained by 4 h after treatment. No recovery of the phytochrome mRNA level was evident by 8 h after benzyladenine treatment. The abundance of actin mRNA was unaffected by benzyladenine treatment.
Cotton
J L
JL
Department of Biology, Colorado State University, Ft. Collins 80523.
Ross
C W
CW
Byrne
D H
DH
Colbert
J T
JT
D000199:Actins / Q000235:genetics; D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D001593:Benzyl Compounds; D003583:Cytokinins / Q000494:pharmacology; D015536:Down-Regulation; D007701:Kinetin; D008027:Light; D010834:Phytochrome / Q000235:genetics*; D010944:Plants / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D011687:Purines; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects
D000199:Actins; D001593:Benzyl Compounds; D003583:Cytokinins; D011687:Purines; D012333:RNA, Messenger; D010834:Phytochrome; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin