Identification of low and high molecular weight follicle-stimulating hormone receptor-binding inhibitors in human follicular fluid.
Various fractions of human follicular fluid (FF) were tested for follicle-stimulating hormone (FSH) receptor-binding inhibitory activity, as well as for their ability to form a dansyl derivative previously reported to be associated with FSH receptor-binding inhibitory activity in bovine FF. Pooled human FF was found to inhibit binding of radioiodinated human FSH (125I-hFSH) to its receptor in a concentration-related manner. Fractionation of human FF by sequential ultrafiltration through membranes of calibrated pore size resulted in human FF components of low (500 to 5,000) and high (greater than 5,000) molecular weight, (Mr) respectively. Each of these inhibited 125I-hFSH binding to receptor in a concentration-related manner. Dansylation of the above components revealed the Rf = 0.15 dansyl derivative to be present in unfractionated human FF and in the low (500 to 5,000 Mr), but not the high (greater than 5,000 Mr), component of human FF. These findings are consistent with those previously reported for bovine FF and porcine FF and indicate that human FF contains FSH receptor-binding inhibitors with properties similar to those observed in animal species.
Lee
D W
DW
Albany Medical College, New York 12208.
Shelden
R M
RM
Reichert
L E
LE
D000818:Animals; D001826:Body Fluids / Q000378:metabolism*; D002417:Cattle; D002855:Chromatography, Thin Layer; D003619:Dansyl Compounds / Q000378:metabolism; D005260:Female; D005640:Follicle Stimulating Hormone / Q000037:antagonists & inhibitors*; D006801:Humans; D008970:Molecular Weight; D006080:Ovarian Follicle / Q000378:metabolism*; D011869:Radioligand Assay; D011962:Receptors, FSH / Q000378:metabolism*
D003619:Dansyl Compounds; D011962:Receptors, FSH; D005640:Follicle Stimulating Hormone