Rhizocticin A, an antifungal phosphono-oligopeptide of Bacillus subtilis ATCC 6633: biological properties.
Rhizocticin A, the main component of the antifungal, hydrophilic phosphono-oligopeptides of Bacillus subtilis ATCC 6633, was used for sensitivity testing and experiments into the molecular mechanism of the antibiotic action. Budding and filamentous fungi as well as the cultivated nematode Caenorhabditis elegans were found to be sensitive, whereas bacteria and the protozoon Paramecium caudatum were insensitive. Rhizoctonia solani was inhibited in agar dilution tests but not in diffusion tests. The antifungal effect of rhizocticin A was neutralized by a variety of amino acids and oligopeptides. Oligopeptide influence was mainly understood as transport antagonism, and it was concluded that the antibiotic enters the recipient cell via the peptide transport system. L- and D-cystine were also identified as potent, general antagonists of the oligopeptide transport. The rhizocticin-antagonism of four other amino acids was taken as a clue to the site of action. Provided that rhizocticin A is split by peptidases of the target cell into inactive L-arginine and toxic L-2-amino-5-phosphono-3-cis-pentenoic acid (L-APPA), the latter may interfere with the threonine or threonine-related metabolism.
Kugler
M
M
Institut für Mikrobiologie I, Universität Tübingen, Federal Republic of Germany.
Loeffler
W
W
Rapp
C
C
Kern
A
A
Jung
G
G
D000596:Amino Acids / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000818:Animals; D000935:Antifungal Agents / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000378:metabolism*; D001419:Bacteria / Q000187:drug effects; D056890:Eukaryota / Q000187:drug effects; D005285:Fermentation; D005658:Fungi / Q000187:drug effects*; D015394:Molecular Structure; D009348:Nematoda / Q000187:drug effects; D009842:Oligopeptides / Q000494:pharmacology; D009943:Organophosphorus Compounds / Q000037:antagonists & inhibitors / Q000494:pharmacology
D000596:Amino Acids; D000935:Antifungal Agents; D009842:Oligopeptides; D009943:Organophosphorus Compounds; C063847:rhizocticin A