title: Resistance mechanisms of multiresistant serotype 012 Pseudomonas aeruginosa isolated in Europe.
abstract: Serotype 012 Pseudomonas aeruginosa resistant to gentamicin (MIC greater than 4 mg/l) and carbenicillin (MIC greater than 128 mg/l) occur widely in Europe and are homogeneous in their phenotypic and genetic properties. It has been suggested that a single multiresistant strain of this serotype has become widespread. This study examined the resistance mechanisms present in multiresistant serotype 012 P. aeruginosa isolates from Austria, Belgium, France, Greece, Italy, Holland, West Germany and the UK. Disseminated isolates produced a PSE-1 type beta-lactamase, correlating with their resistance to the known substrates of this enzyme (carbenicillin, azlocillin and cefsulodin). These isolates also reacted with gene probes for the aminoglycoside modifying enzymes AAC(6')I and ANT(3'). The probe for AAC(6')I is known to cross-react with the gene for AAC(6')II. The fact that the organisms were resistant to netilmicin, gentamicin, sisomicin and tobramycin, but less so to amikacin, suggested that the latter enzyme was produced rather than AAC(6')I. PSE-1 beta-lactamase and the gene for AAC(6')I/AAC(6')II were absent from the International Antigenic Typing Scheme 012 reference strain, which was sensitive to beta-lactams and aminoglycosides, and also from a multiresistant serotype 012 strain isolated at a London burns unit in 1987. These organisms have been shown previously to be distinct from the disseminated multiresistant strain in their phenotypic properties. Two 012 isolates appeared to have additional resistance determinants to amikacin and isepamicin.
authors.affiliation: Division of Hospital Infection, Central Public Health Laboratory, London, UK.
authors.forename: T L
authors.initials: TL
authors.lastname: Pitt
authors.forename: D M
authors.initials: DM
authors.lastname: Livermore
authors.forename: G
authors.initials: G
authors.lastname: Miller
authors.forename: A
authors.initials: A
authors.lastname: Vatopoulos
authors.forename: N J
authors.initials: NJ
authors.lastname: Legakis
mesh_terms: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D005060:Europe; D009693:Nucleic Acid Hybridization / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000201:enzymology / Q000378:metabolism*; D013045:Species Specificity; D001618:beta-Lactamases / Q000096:biosynthesis*; D047090:beta-Lactams
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases