Dipalmitoylphosphatidylcholine (L-alpha-lecithin) stimulates phospholipase A2 activity in human amnion.
In order to investigate the mechanism of dipalmitoylphosphatidylcholine (DPPC, L-alpha-lecithin) stimulation of the prostaglandin E (PGE) production of the amniotic membrane, effects of DPPC (50-800 micrograms/ml) on phospholipase A2 (PLA2), phospholipase C (PLC), PG endoperoxide synthase, and PGE synthase activities of human amniotic membrane were studied. Only PLA2 activity was increased by DPPC, suggesting that lecithin, the major surfactant component, increases the PGE production of the amniotic membrane by activating PLA2.
Ohtsuka
T
T
Department of Obstetrics and Gynecology, Miyazaki Medical College, Japan.
Lee
H C
HC
Mibe
M
M
Nabekura
H
H
Yamaguchi
M
M
Mori
N
N
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000494:pharmacology*; D000650:Amnion / Q000187:drug effects / Q000201:enzymology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D011247:Pregnancy; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D011458:Prostaglandins E / Q000096:biosynthesis*; D010738:Type C Phospholipases / Q000378:metabolism
D011458:Prostaglandins E; D015060:1,2-Dipalmitoylphosphatidylcholine; D011451:Prostaglandin-Endoperoxide Synthases; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D010738:Type C Phospholipases