The structure of the human synapsin I gene and protein.
Synapsin I is a peripheral membrane protein of synaptic vesicles that mediates their attachment to the cytoskeleton. Human genomic clones containing the full coding sequence of synapsin I were isolated, and the exons were mapped and sequenced. Human synapsin I is encoded by a single copy gene containing 13 exons ranging in size from 58 base pairs to more than 1 kilobase that are unequally distributed over more than 30 kilobases of DNA on the X-chromosome. The differential splicing of the primary synapsin I transcript that generates synapsins Ia and Ib involves alternative use of splice acceptor sites at the last intron-exon boundary. The primary structure of synapsin I is highly conserved between the human, rat, and bovine proteins (95% identity). The intron placement within that primary structure correlates with the previously postulated domain model of the protein. Exon I contains domains A and B, while exon 12 contains almost all of domain D, and exon 13 contains the alternatively spliced domains E and F. Domain C, the central homologous domain implicated in the binding of synapsin I to actin and to synaptic vesicles, is divided into nine exons.
Südhof
T C
TC
Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas 75235.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005091:Exons; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D016704:Synapsins
D009419:Nerve Tissue Proteins; D016704:Synapsins; D004247:DNA