[Formation of virus-like particles by HIV-1 Gag proteins, expressed by a recombinant vaccinia virus].
Monkey kidney cells CV-1 were infected with recombinant vaccinia virus carrying HIV-1 gag gene with a deletion of 230 nucleotide pairs from the 3'-terminus. The main gene product detected in the lysates of infected cells was the gag precursor rp50. The protein was accumulated on the cell membranes suggesting that it had a myristylated N-terminus, and was cleaved by a recombinant virus specific protease with the formation of two proteins, p17 and p24 corresponding in molecular masses to mature gag proteins. Virus-like particles similar to immature HIV virions were budding from the surface of infected cells. They look like the ring of optically dense material covered with a lipid bilayer, of the same size (100-120 nm) and of the same density in a sucrose gradient (1.16-1.18 g/ml) as HIV-1 virions. The particles contained rp50 and cellular heterogeneous RNA. Thus, the unprocessed gag precursor with deleted 77 amino acid residues from the C-terminus is able to form virus-like particles in the absence of env proteins and virus-specific RNA, and these particles are budding from the cell surface. The question about the use of extracellular Gag-particles for AIDS diagnostic work and construction of vaccines is discussed.
Vzorov
A N
AN
Tentsov
Iu Iu
IuIu
Grigor'ev
V B
VB
Shulenin
S A
SA
Garaev
M M
MM
Bogdan
O P
OP
Bukrinskaia
A G
AG
D015152:Blotting, Northern; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D015967:Gene Expression Regulation, Viral; D015683:Gene Products, gag / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D006868:Hydrolysis; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis; D011995:Recombination, Genetic; D014616:Vaccinia virus / Q000235:genetics*; D014771:Virion
D015683:Gene Products, gag; D012367:RNA, Viral
Obrazovanie virusopodobnykh chastits belkov Gag VICh-1, ékspressiruemym rekombinantnym virusom ospovaktsiny.