Expression of IgE from a nonrearranged epsilon locus in cloned B-lymphoblastoid cells that also express IgM.
During development, B lymphocytes have the ability to switch from synthesis of IgM to immunoglobulins of another isotype such as IgG, IgA, or IgE. This class switching mechanism has been shown to involve DNA rearrangement and concomitant deletion of intervening CH genes. In our report, an EBV-transformed B lymphoblastoid cloned cell line is described that simultaneously expressed and secreted both IgM and IgE. DNA analysis showed the (nonproductive) rearrangement of one allele to gamma and (productive) rearrangement of the other allele to mu. Germ-line arrangement of the C epsilon gene was preserved on both alleles.
Chan
M A
MA
Division of Basic Sciences, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.
Benedict
S H
SH
Dosch
H M
HM
Hui
M F
MF
Stein
L D
LD
D001402:B-Lymphocytes / Q000502:physiology*; D015152:Blotting, Northern; D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism; D015870:Gene Expression; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D005803:Genes, Immunoglobulin; D006801:Humans; D007073:Immunoglobulin E / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007075:Immunoglobulin M / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011233:Precipitin Tests; D012333:RNA, Messenger / Q000235:genetics
D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D012333:RNA, Messenger; D007073:Immunoglobulin E