A promoter that drives transgene expression in cerebellar Purkinje and retinal bipolar neurons.
A genomic clone encoding the Purkinje cell-specific L7 protein has been isolated and utilized to drive the expression of beta-galactosidase in mice. Three independent transgenic lines, germ line transformed with an L7-beta-galactosidase fusion gene, exhibit beta-galactosidase expression in both cerebellar Purkinje cells and retinal bipolar neurons. This distribution is the same as that previously determined for the L7 protein by immunohistochemistry. The transgenic murine lines can be used to obtain populations of marked Purkinje and bipolar neurons. Similar L7 promoter constructs can be used to express other foreign genes specifically in these two classes of neurons.
Oberdick
J
J
Department of Neuroscience, Roche Research Center, Nutley, NJ 07110.
Smeyne
R J
RJ
Mann
J R
JR
Zackson
S
S
Morgan
J I
JI
D000818:Animals; D002531:Cerebellum / Q000378:metabolism; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D005696:Galactosidases / Q000235:genetics*; D015870:Gene Expression; D015723:Gene Library; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011689:Purkinje Cells / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000032:analysis; D015183:Restriction Mapping; D012160:Retina / Q000378:metabolism*; D012165:Retinal Ganglion Cells / Q000378:metabolism*; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics*
D009419:Nerve Tissue Proteins; C091104:Purkinje cell protein L7; D011993:Recombinant Fusion Proteins; D005696:Galactosidases; D001616:beta-Galactosidase