Ultrastructural effects of Nifurtimox on rat adrenal cortex related to reductive biotransformation.
Nifurtimox (Nfx) (4(5-nitrofurfurylidene)amino)-3-methylthiomorpholine-1, 1-dioxide) is a drug used against Chagas' disease, a parasitic sickness afflicting several million Latin Americans. Nfx administration to Sprague-Dawley male rats (220-250 g) at a dose of 100 mg/kg caused pronounced alterations in the adrenal cortex involving the fasciculata and reticularis zones but which were not evident in the glomerulosa. Alterations observed involved mitochondria, nuclei, Golgi apparatus, and the endoplasmic reticulum but were more intense in the mitochondria. There is Nfx nitroreductase activity in the adrenal microsomal, mitochondrial, and cytosolic-rich fractions but most of it is in the mitochondrial-rich fraction. Activity in the first two fractions requires NADPH and that in the cytosol is only observed in the presence of hypoxanthine as substrate. Enzymatic activity in all fractions is inhibited by oxygen. CO does not inhibit mitochondrial Nfx nitroreductase and inhibits only 10% of the microsomal enzyme activity. Hypoxanthine-dependent cytosolic activity is inhibited by allopurinol. Present results suggest that Nfx is activated to damage-producing reactive metabolites by nitroreductive biotransformation in rat adrenal organelles. Mitochondrial and microsomal bioactivation would occur at the level of the flavoenzyme P-450 reductase rather than at P-450 itself, and cytosolic bioactivation would be mediated by xanthine oxidase. Epidemiological studies on adrenal function in patients undergoing Nfx treatment would be necessary to establish the potential toxicological relevance of these findings.
de Castro
C R
CR
Centro de Investigaciones Toxicológicas (CEITOX) CITEFA/CONICET, Provincia de Buenos Aires, Argentina.
De Toranzo
E G
EG
Carbone
M
M
Castro
J A
JA
D000302:Adrenal Cortex / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D000818:Animals; D001711:Biotransformation / Q000187:drug effects / Q000502:physiology; D002245:Carbon Dioxide / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology; D008297:Male; D008854:Microscopy, Electron; D008861:Microsomes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D009547:Nifurtimox / Q000378:metabolism / Q000493:pharmacokinetics / Q000494:pharmacology*; D009581:Nitrofurans / Q000494:pharmacology*; D009584:Nitrogen / Q000378:metabolism; D009601:Nitroreductases / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D009581:Nitrofurans; D002245:Carbon Dioxide; C029492:aromatic NADH-dependent nitroreductase; D009601:Nitroreductases; D009547:Nifurtimox; D009584:Nitrogen; D010100:Oxygen