Nopaline synthase gene is expressed in Escherichia coli and in cucumber cells under a hybrid promoter.
Fusion of the nopaline synthase gene (nos) to the Escherichia coli trp promoter gave rise to a hybrid promoter (tros). Under control of this hybrid element, synthesis of nopaline was observed in E. coli as well as in cucumber cells transformed with the described vector.
Gafni
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Department of Plant Genetics and Breeding, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel.
D000594:Amino Acid Oxidoreductases / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014170:Transformation, Genetic
D000594:Amino Acid Oxidoreductases; C021883:nopaline synthase