Enzymatic synthesis of purine 2'-deoxyriboside and its properties as an inhibitor of adenosine deaminases from calf intestinal mucosa and Bacillus cereus.
Enzymatic synthesis of purine 2'-deoxyriboside was obtained by reacting purine with excess 2-deoxy-alpha-D-ribose-1-phosphate in the presence of commercial bovine nucleoside phosphorylase; the product was isolated by semipreparative reverse phase HPLC with an overall 62% yield. Purine 2'-deoxyriboside was shown to behave as a competitive inhibitor of adenosine deaminase from calf intestinal mucosa and Bacillus cereus, with apparent Ki values of 4.5 and 8.5 microM, respectively.
Caffaz
S
S
Dipartimento di Fisiologia e Biochimica, Laboratori di Biochimica, Università, Pisa.
Allegrini
S
S
Cercignani
G
G
D058892:Adenosine Deaminase Inhibitors; D000818:Animals; D001409:Bacillus cereus / Q000201:enzymology; D002417:Cattle; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000201:enzymology; D007700:Kinetics; D009700:Nucleoside Deaminases / Q000037:antagonists & inhibitors*; D011684:Purine Nucleosides / Q000096:biosynthesis* / Q000494:pharmacology; D012263:Ribonucleosides / Q000096:biosynthesis* / Q000494:pharmacology; D012267:Ribosemonophosphates
D058892:Adenosine Deaminase Inhibitors; D011684:Purine Nucleosides; D012263:Ribonucleosides; D012267:Ribosemonophosphates; C031302:2-deoxyribose 1-phosphate; C005248:nebularine; D009700:Nucleoside Deaminases