Measurement of intracellular Na in the rat salivary gland: a 23Na-NMR study using double quantum filtering.
23Na in the prefused rat mandibular salivary gland was measured by spin-echo double quantum filter 23Na-NMR spectroscopy at 8.45 T. Resonances due to the intracellular 23Na and the interstitial 23Na were observed in the perfused gland at 25 degrees C. The resonance due to intracellular 23Na consisted of two Lorentzian signals stemming from the [1/2 mean value of -1/2[ coherence (sharp resonance) and the [-1/2 mean value of -3/2[ and [3/2 mean value of 1/2[ coherences (broad resonance). The transverse relaxation rate constant corresponding to the [1/2 mean value of -1/2[ coherence was 95 +/- 4 s-1 and that corresponding to the [-1/2 mean value of -3/2[ and [3/2 mean value of 1/2[ coherences was 1360 +/- 75 s-1 (mean +/- S.E., n = 5). The resonance due to the interstitial 32Na had longer relaxation rate constants, and disappeared upon administration of dysprosium triethylenetetramine-N,N',N",N",N"'-hexaacetic acid.
Seo
Y
Y
Department of Molecular Physiology, National Institute for Physiological Sciences, Okazaki, Japan.
Murakami
M
M
Watari
H
H
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D004419:Dysprosium; D004492:Edetic Acid / Q000031:analogs & derivatives; D009682:Magnetic Resonance Spectroscopy; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012469:Salivary Glands / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism; D012964:Sodium / Q000032:analysis* / Q000378:metabolism
D004419:Dysprosium; D010042:Ouabain; C026059:triethylenetetraminehexaacetic acid; D004492:Edetic Acid; D012964:Sodium; D000109:Acetylcholine