Distribution of gonococcal lipopolysaccharide biosynthesis genes among strains of Neisseria gonorrhoeae and other neisserial species.
A plasmid, pTME6, containing Neisseria gonorrhoeae lipopolysaccharide biosynthesis genes was used as a probe to analyze DNA from strains of N. gonorrhoeae, N. meningitidis and various commensal Neisseria by Southern blotting. Chromosomal DNA from 26 gonococcal strains probed with 32P-labeled pTME6 produced five different hybridization patterns. No correlation between hybridization pattern and auxotype, serotype, serum sensitivity or SDS-urea-PAGE migration of LPS was observed. DNA from strains of N. meningitidis, N. lactamica and N. cinerea, but not other commensal Neisseria species, hybridized strongly to pTME6.
Palermo-Dilts
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DA
Department of Microbiology and Immunology, School of Medicine and Dentistry, University of Rochester, NY 14642.
Silver
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LE
Clark
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VL
D004269:DNA, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D008070:Lipopolysaccharides / Q000096:biosynthesis / Q000235:genetics*; D009343:Neisseria / Q000235:genetics*; D009344:Neisseria gonorrhoeae / Q000235:genetics* / Q000254:growth & development; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D013291:Streptococcus / Q000235:genetics* / Q000254:growth & development
D004269:DNA, Bacterial; D008070:Lipopolysaccharides