Identification of ADPR-transferase activity in the rat tapeworm, Hymenolepis diminuta.
1. The nuclear fraction of the rat tapeworm Hymenolepis diminuta (Cestoda) contains the enzyme adenosine diphosphoribosyl transferase (ADPR-transferase). 2. The enzyme catalyzes the postsynthetic modification of some nuclear proteins by the covalent attachment of the (ADP-ribose) moiety of NAD to such proteins. 3. The reaction is dependent on DNA which contains strand-breaks, and chain lengths equivalent to (ADP-ribose) is estimated. 4. The formation of polynucleotide products was competitively inhibited by 3-acetamidobezamide, with a Km of 125 microM. 5. The catalytic properties of ADPR-transferase in Hymenolepis diminuta are similar to those in T. brucei.
Alsharif
M
M
Department of Biochemistry, University of Bath, UK.
Apweiler
R
R
Whish
W J
WJ
Branford White
C J
CJ
Brunt
R V
RV
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000031:analogs & derivatives / Q000378:metabolism; D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D002467:Cell Nucleus / Q000201:enzymology; D006926:Hymenolepis / Q000201:enzymology*; D007700:Kinetics; D008297:Male; D009243:NAD / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D051381:Rats
D001549:Benzamides; D009687:Nuclear Proteins; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D009243:NAD; C016664:2'-(5''-phosphoribosyl)-5'-adenosine monophosphate; D000246:Adenosine Diphosphate Ribose; D000249:Adenosine Monophosphate; C040561:3-acetamidobenzamide; D011065:Poly(ADP-ribose) Polymerases