Production and characterization of monoclonal antibodies specific for bovine gamma-interferon.
Nine stable hybridoma cell lines were established which secreted specific monoclonal antibodies (MAbs) to bovine gamma-interferon (BoIFN-gamma). Specific binding of each of the MAbs to recombinant BoIFN-gamma (rBoIFN-gamma) was demonstrated in an indirect ELISA, whilst none of the MAbs bound to rBoIFN-alpha or rBoIFN-beta. In a Western blot the MAbs reacted with the 16 kDa and 32 kDa polypeptides present in rBoIFN-gamma preparations. Competitive ELISA's showed that four MAbs bound to one epitope on rBoIFN-gamma, and the other five MAbs bound to a separate epitope. Two MAbs, each recognising different epitopes, were shown to neutralise the anti-viral activity of natural BoIFN-gamma.
Wood
P R
PR
CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Vic., Australia.
Rothel
J S
JS
McWaters
P G
PG
Jones
S L
SL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D002417:Cattle / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D007124:Immunoenzyme Techniques / Q000662:veterinary; D007370:Interferon Type I / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology* / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D011994:Recombinant Proteins; D012672:Semliki forest virus / Q000187:drug effects
D000911:Antibodies, Monoclonal; D007370:Interferon Type I; D011994:Recombinant Proteins; D007371:Interferon-gamma