Histochemical identification of oestrogen target cells in the medullary bone of laying hens.
1. Oestrogen target cells in the medullary bone of laying hens were examined using a histochemical method with fluorescein isothiocyanate labelled oestradiol. 2. Specific fluorescence was found to occur strongly in osteoblasts and weakly in osteoclasts on the medullary bone surface. 3. This study suggests that osteoblasts on the medullary bone surface are oestrogen target cells.
Ohashi
T
T
Department of Animal Science, Faculty of Agriculture, Niigata University, Japan.
Kusuhara
S
S
Ishida
K
K
D000818:Animals; D001842:Bone and Bones / Q000032:analysis / Q000166:cytology*; D002645:Chickens / Q000378:metabolism*; D004958:Estradiol / Q000032:analysis; D004967:Estrogens / Q000378:metabolism*; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D006651:Histocytochemistry; D008856:Microscopy, Fluorescence; D013861:Thiocyanates
D004967:Estrogens; D005452:Fluoresceins; D013861:Thiocyanates; D004958:Estradiol; D016650:Fluorescein-5-isothiocyanate