title: Multivariate analysis of quantitative chemical and enzymic characterization data in classification of Actinobacillus, Haemophilus and Pasteurella spp.
abstract: Chemotaxonomic data for strains of Actinobacillus, Haemophilus and Pasteurella spp. were analysed using three multivariate statistical strategies: principal components, partial least squares discriminant, and soft independent modelling of class analogy. The species comprised Actinobacillus actinomycetemcomitans. Haemophilus aphrophilus, H. paraphrophilus, H. influenzae, Pasteurella multocida, P. haemolytica and P. ureae. Strains were characterized by cell sugar and fatty acid composition, lysis kinetics during EDTA and EDTA plus lysozyme treatment, and methylene blue reduction. In total 23 quantitative variables were compiled from chemotaxonomic analyses of 25 strains. A. actinomycetemcomitans and H. aphrophilus formed distinct classes which differed from those of H. paraphrophilus, H. influenzae and Pasteurella spp. All characterization variables, except those describing fatty acid content, contributed significantly to inter-species discrimination.
authors.affiliation: Research Department, National Institute of Occupational Health, Umeå, Sweden.
authors.forename: I
authors.initials: I
authors.lastname: Brondz
authors.forename: I
authors.initials: I
authors.lastname: Olsen
authors.forename: M
authors.initials: M
authors.lastname: Sjöström
mesh_terms: D000188:Actinobacillus / Q000145:classification* / Q000187:drug effects / Q000378:metabolism; D002241:Carbohydrates / Q000032:analysis; D016002:Discriminant Analysis; D004492:Edetic Acid / Q000494:pharmacology; D005227:Fatty Acids / Q000032:analysis; D006190:Haemophilus / Q000145:classification* / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D008751:Methylene Blue / Q000378:metabolism; D015999:Multivariate Analysis; D009113:Muramidase / Q000494:pharmacology; D010325:Pasteurella / Q000145:classification* / Q000187:drug effects / Q000378:metabolism; D009626:Terminology as Topic
chemical_list: D002241:Carbohydrates; D005227:Fatty Acids; D004492:Edetic Acid; D009113:Muramidase; D008751:Methylene Blue