title: [Production and characterization of a high affinity monoclonal antibody with digoxin and digitoxin specificity].
abstract: A monoclonal antibody with a high affinity for digoxin (KA = 5.5 x 10(10) M-1) and digitoxin KA = 5.0 x 10(10) M-1) was produced by somatic cell fusion. This antibody, designated 2A3, displayed little cross reactivity with other glucosides and no cross reactivity with endogenous steroids. It was shown that 2A3 is a suitable tool in an enzyme immunoassay for digoxin and digitoxin.
authors.affiliation: Institut für Klinische Immunologie, Friedrich-Schiller-Universität Jena.
authors.forename: H U
authors.initials: HU
authors.lastname: Simon
authors.forename: W
authors.initials: W
authors.lastname: Hubl
authors.forename: W D
authors.initials: WD
authors.lastname: Müller
authors.forename: I
authors.initials: I
authors.lastname: Wolf
authors.forename: G
authors.initials: G
authors.lastname: Schlenvoigt
authors.forename: L
authors.initials: L
authors.lastname: Jäger
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D003429:Cross Reactions; D004074:Digitoxin / Q000276:immunology*; D004077:Digoxin / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay
chemical_list: D000911:Antibodies, Monoclonal; D004077:Digoxin; D004074:Digitoxin
vernacular_title: Produktion und Charakterisierung eines hochaffinen monoklonalen Antikörpers mit Digoxin- und Digitoxin-Spezifität.