Gram-negative bacteria sepsis in the rat and tissue lipolytic activity on LCT and MCT/LCT-based commercial parenteral emulsions.
The aim of this study was to evaluate the effect of a gram-negative bacteria sepsis on the activity of the enzymes lipoprotein lipase (LPL) and hepatic lipase (HL), involved in the clearance of circulating triacylglycerol-rich fat particles. Fasting rats were intravenously injected with NaCl9 g.l-1, live or heat-killed Pseudomonas aeruginosa bacteria. After 18 h the animals were killed. When compared to controls, the 2 treated groups showed an increase in body temperature, cholesterolemia, triglyceridemia and a decrease in ketonemia, proteinemia, albuminemia and in the in vitro activity of diaphragm, heart and adipose tissue LPL and of HL. The decrease in the enzyme activities occurred independent of the type of emulsion used as in vitro substrate, whether it was based on long-chain triglycerides or on medium- and long-chain triglycerides, but in any case the activity was lower with the first than with the second type of fat emulsion.
Meraihi
Z
Z
Laboratoire, Clinique Médicale A, Hôpital Civil, Strasbourg, France.
Lutz
O
O
Scheftel
J M
JM
Frey
A
A
Bach
A C
AC
D000818:Animals; D005217:Fat Emulsions, Intravenous / Q000008:administration & dosage*; D008049:Lipase / Q000097:blood; D008053:Lipid Mobilization / Q000502:physiology*; D008055:Lipids / Q000097:blood; D008071:Lipoprotein Lipase / Q000097:blood; D008099:Liver / Q000201:enzymology; D008297:Male; D010289:Parenteral Nutrition, Total / Q000379:methods*; D011552:Pseudomonas Infections / Q000503:physiopathology*; D051381:Rats; D011919:Rats, Inbred Strains; D018805:Sepsis / Q000503:physiopathology*; D014280:Triglycerides / Q000008:administration & dosage*
D005217:Fat Emulsions, Intravenous; D008055:Lipids; D014280:Triglycerides; D008049:Lipase; D008071:Lipoprotein Lipase