Qualitative and quantitative changes in beta-1,4-glucosidase accompanying growth of Aspergillus nidulans.
A comparative study of the wild type A. nidulans and a mutant strain aco-T69 (lacking conidia and cleistothecia) revealed the better production of beta-1,4-glucosidase in the former. The relative distribution of the enzyme levels in various morphological structures viz. somatic hyphae, spores and cleistothecia also showed a variation. Highest specific activity was found in the cleistothecial extracts. The electrophoretic analysis of the wild type strain demonstrated the presence of three isoenzymes of beta-1,4-glucosidase i.e. beta-GLU I, beta-GLU II and beta-GLU III, the number of which varies from one to three during the growth of the organism. The parallel study of the mutant strain showed complete absence of beta-GLU II. All three forms were present in the somatic hyphae and cleistothecial extracts while spore extracts depicted the absence of beta-GLU III in the wild type.
Sharma
S
S
School of Life Sciences, Guru Nanak Dev University, Amritsar, India.
Bagga
P S
PS
Sandhu
D K
DK
D001230:Aspergillus / Q000201:enzymology* / Q000254:growth & development; D007527:Isoenzymes / Q000032:analysis; D001617:beta-Glucosidase / Q000032:analysis*
D007527:Isoenzymes; D001617:beta-Glucosidase