Peripheral B-lymphocyte markers and function in IgA nephropathy.
Peripheral blood B-lymphocyte markers and functions were observed in 21 patients with IgA nephropathy (IgA NP) and in 16 controls. IgA NP B lymphocytes expressed significantly higher positivity with Leu 1 (CD 5) monoclonal antibody than controls. CD 5 positive B lymphocytes are thought to be a distinct subset of the B cells (autoregulatory B lymphocytes) inducible in IgA NP by lipopolysaccharide (LPS) stimulation parallel to the higher expression of surface IgM heavy chain positivity. The activated state of IgA NP B lymphocytes has been proved by their higher OKIa (HLA-DR) positivities but lower IOB1a (CD 21, C3d-receptor) and decreased IgG-Fc-receptor (ox-rosette) expression. IgA NP B lymphocytes showed a higher IgA but also IgG and IgM polyclonal immunoglobulin production than control B lymphocytes in co-cultures with T lymphocytes. Not only regulatory T lymphocyte subsets but also serum derived from IgA NP patients stimulated the immunoglobulin production of IgA NP B cells.
Magyarlaki
T
T
Department of Pathology, University Medical School of Pécs, Hungary.
Davin
J C
JC
Szabados
E
E
Kocsis
B
B
Nagy
J
J
D000328:Adult; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000032:analysis / Q000276:immunology*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005922:Glomerulonephritis, IGA / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000032:analysis; D007143:Immunoglobulin Heavy Chains / Q000032:analysis / Q000276:immunology; D007075:Immunoglobulin M / Q000032:analysis; D008297:Male; D008875:Middle Aged; D011043:Pokeweed Mitogens; D011092:Polyethylene Glycols; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000032:analysis / Q000276:immunology
D000911:Antibodies, Monoclonal; D007070:Immunoglobulin A; D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D011043:Pokeweed Mitogens; D011092:Polyethylene Glycols