Activation of promutagenic chemicals by Streptomyces griseus containing cytochrome P-450soy.
Streptomyces griseus cells containing cytochrome P-450soy oxidize a diverse array of xenobiotic compounds. This metabolic capability was exploited for activation of promutagenic chemicals such as polycyclic aromatic hydrocarbons, aromatic amines and small aliphatics in a modified Salmonella/Ames plate incorporation assay using tester strains TA98 and TA1538. In this assay promutagens such as 3,3'-dimethylbenzidine, 3,3'-dimethoxybenzidine, benzidine, 2-acetylaminofluorene, 2-aminoanthracene, 2,4-diaminotoluene, 4-aminobiphenyl, benzo(a)pyrene, chloropicrin and N-nitrosodimethylamine were oxidized to mutagenic metabolites by S. griseus intact cells which mutated Salmonella tester strains (TA98 and TA1538). S. griseus failed to activate 7,12-dimethylbenzanthracene and 4-chloro-2-nitroaniline. In parallel tests performed with rat liver homogenate (S9), N-nitrosodimethylamine was not activated.
Sariaslani
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FS
Central Research & Development Dept., E.I. duPont de Nemours & Co., Wilmington, Delaware 19880-0228.
Stahl
R G
RG
D001711:Biotransformation; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004789:Enzyme Activation; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000378:metabolism*; D013025:Glycine max; D013305:Streptomyces griseus / Q000378:metabolism*
D009153:Mutagens; D003577:Cytochrome P-450 Enzyme System