Cloning and sequencing of the class B beta-lactamase gene (ccrA) from Bacteroides fragilis TAL3636.
Bacteroides fragilis TAL3636 produces a class B, Zn2(+)-requiring beta-lactamase. The gene, ccrA, was cloned and expressed in Escherichia coli. The gene was sequenced and shown to share greater than 33% identity with the metalloenzyme from Bacillus cereus 569/H.
Rasmussen
B A
BA
Department of Molecular Biology, American Cyanamid Company, Pearl River, New York 10965.
Gluzman
Y
Y
Tally
F P
FP
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001409:Bacillus cereus / Q000201:enzymology; D001441:Bacteroides fragilis / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D001618:beta-Lactamases / Q000235:genetics*; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases