Alkaline induction of a novel gene locus, alx, in Escherichia coli.
A novel pH-regulated locus inducible over 100-fold in alkaline media was identified in Escherichia coli through screening of 93,000 Mu dI1734 (lacZ Kmr) operon fusions at pH 6.5 and pH 8.5. Four lacZ fusions that showed expression only at the higher pH were mapped at 67.5 min by P1 transduction crosses. The locus was designated alx.
Bingham
R J
RJ
Department of Biology, Kenyon College, Gambier, Ohio 43022.
Hall
K S
KS
Slonczewski
J L
JL
D003433:Crosses, Genetic; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009876:Operon; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D014161:Transduction, Genetic; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase