Immobilized carbonic anhydrase in a membrane lung for enhanced CO2 removal.
The potential use of carbonic anhydrase (CA), immobilized within membrane lungs, to accelerate the removal of CO2 from blood was investigated. Using a variation on the technique of Chang, 500 micrograms/mL of CA was encapsulated in 5-20 microns cellulose nitrate microcapsules which were then immobilized onto a 0.1 m2 silicone rubber membrane. Using an in vitro test circuit, 0.68 mmol/m2/min of CO2 was removed from venous blood through an untreated membrane at steady state. With the CA-immobilized membrane, CO2 removal was enhanced to 1.09 mmol/m2/min. This technique has the potential of greatly improving the CO2 efficiency of commercial membrane lungs for ECCOR.
Salley
S O
SO
Department of Chemical Engineering, Wayne State University, Detroit, MI.
Song
J Y
JY
Whittlesey
G C
GC
Klein
M D
MD
D002245:Carbon Dioxide / Q000097:blood*; D002256:Carbonic Anhydrases; D004800:Enzymes, Immobilized; D004867:Equipment Design; D015199:Extracorporeal Membrane Oxygenation / Q000295:instrumentation*; D006801:Humans; D010107:Oxygenators, Membrane; D010316:Particle Size; D013499:Surface Properties
D004800:Enzymes, Immobilized; D002245:Carbon Dioxide; D002256:Carbonic Anhydrases