Synthesis and biological activities of 8-substituted 2-5A analogues.
The 8-(2-hydroxypropyl)-adenosine and 8-hydroxy adenosine-substituted analogues of 2-5A and it's derivatives were synthesized and their biological activity was evaluated in mouse L cell extracts. The 8-hydroxy adenosine-substituted analogues (i.e. pppAOH2'p5AOH2'p5'AOH, pAOH2'p5'AOH2'p5'AOH, pppA2'p5'A2'p5'AOH, pA2'p5'A2'p5'AOH) inhibited protein synthesis with a relative activity compared to the parent 2-5A. Further, the greater interest is the observation that the corresponding 5'-monophosphate had to inhibitory activity. However, 8-(2-hydroxypropyl)-adenosine substituted analogue (pAHPr2'p5'AHPr2'p5'AHPr) can not about bound as well as parent 2-5A.
Nagai
K
K
Department of Industrial Chemistry, Chiba Institute of Technology, Japan.
Yokoyama
S
S
Kawai
G
G
Shuhadlnik
R J
RJ
Takaku
H
H
D000227:Adenine Nucleotides / Q000737:chemistry* / Q000494:pharmacology; D000241:Adenosine / Q000031:analogs & derivatives / Q000737:chemistry; D000818:Animals; D004722:Endoribonucleases / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D007739:L Cells; D051379:Mice; D009843:Oligoribonucleotides / Q000737:chemistry* / Q000494:pharmacology; D012335:RNA, Ribosomal / Q000378:metabolism
D000227:Adenine Nucleotides; D009843:Oligoribonucleotides; D012335:RNA, Ribosomal; C070276:8-(2-hydroxypropyl)adenosine; C036009:8-oxoadenosine; C023505:2',5'-oligoadenylate; D004722:Endoribonucleases; C031956:2-5A-dependent ribonuclease; D000241:Adenosine