Interferon gamma is involved in the recovery of athymic nude mice from recombinant vaccinia virus/interleukin 2 infection.
Athymic nude mice recover from an infection with recombinant vaccinia virus (VV) encoding murine interleukin 2 (IL-2), but treatment with a mAb to IL-2 accentuated infection. Administration of a mAb against interferon gamma (IFN-gamma) to mice infected with the IL-2-encoding virus completely prevented the IL-2-induced mechanisms of recovery. Both asialo-GM1+ (NK) and asialo-GM1- (non-NK) cells were participants in the IFN-gamma-mediated recovery of nude mice from infection with the IL-2-encoding VV recombinant. Depletion of asialo-GM1+ cells exacerbated infection, though not as much as anti-IFN-gamma mAb. In vitro, both asialo-GM1+ and asialo-GM1- nude mouse splenocytes produced IFN-gamma in response to IL-2.
Karupiah
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Division of Cell Biology, John Curtin School of Medical Research, Australian National University, Canberra, ACT.
Blanden
R V
RV
Ramshaw
I A
IA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000502:physiology; D002452:Cell Count; D005260:Female; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D007376:Interleukin-2 / Q000276:immunology / Q000502:physiology*; D007694:Killer Cells, Natural / Q000378:metabolism; D008168:Lung / Q000378:metabolism / Q000382:microbiology; D051379:Mice; D008819:Mice, Nude / Q000382:microbiology*; D010053:Ovary / Q000378:metabolism / Q000382:microbiology; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D014615:Vaccinia / Q000188:drug therapy* / Q000401:mortality; D014616:Vaccinia virus / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology*; D014779:Virus Replication / Q000187:drug effects / Q000502:physiology
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D007371:Interferon-gamma