A unique mRNA species for the alpha subunit of Gs is present in rat haploid germ cells.
The 1.9 kb mRNA encoding the alpha subunit of Gs observed in the somatic cells of the rat testis was not seen in the germ cells (pachytene spermatocytes and round spermatids) as revealed by Northern blot analysis. Interestingly, the cDNA probe for Gs alpha detected a mRNA of shorter chain length (0.9 kb) exclusively in the haploid germ cells (round and elongated spermatids). However, no ADP ribosylated protein was detected in membranes from germ cells after cholera toxin treatment. The 0.9 kb transcript may represent a haploid germ cell-specific variant of Gs alpha, which is functionally different from the stimulatory component of the adenylate cyclase system.
Haugen
T B
TB
Institute of Medical Biochemistry, University of Oslo, Norway.
Paulssen
E J
EJ
Gordeladze
J O
JO
Hansson
V
V
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D037361:Adenylate Cyclase Toxin; D000367:Age Factors; D000818:Animals; D015152:Blotting, Northern; D002772:Cholera Toxin / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D037342:Pertussis Toxin; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D013087:Spermatids / Q000502:physiology*; D013737:Testis / Q000502:physiology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D037361:Adenylate Cyclase Toxin; D012333:RNA, Messenger; D010566:Virulence Factors, Bordetella; D000246:Adenosine Diphosphate Ribose; D002772:Cholera Toxin; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins