Effect of prolactin on phosphatidylcholine hydrolysis via phospholipase C in isolated adrenocortical cells of guinea pig.
Prolactin significantly increased the rate of incorporation of [3H]choline into phosphorylcholine (PRC) in a purified suspension of guinea pig adrenocortical cells. The rate of phosphatidylcholine (PTC) labelling and cellular PTC content did not change. In cells prelabelled with [3H]choline or CDP [14C]choline, prolactin diminished the rate of reduction of the radioactive PRC pool after 60-90 min incubation without any change in the rate of PTC biosynthesis. Taken together, these findings suggest that prolactin stimulates the hydrolysis of PTC by phospholipase C into PRC and diacylglycerol. The significance of this effect as part of the mechanism of action of prolactin on adrenocortical cells is discussed.
Sautin YuY
Institute of Endocrinology and Metabolism, Kiev, USSR.
Tronko
N D
ND
Mikosha
A S
AS
D000302:Adrenal Cortex / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D004075:Diglycerides / Q000378:metabolism; D006168:Guinea Pigs; D006868:Hydrolysis; D007700:Kinetics; D010713:Phosphatidylcholines / Q000378:metabolism*; D010767:Phosphorylcholine / Q000378:metabolism; D011388:Prolactin / Q000502:physiology*; D010738:Type C Phospholipases / Q000378:metabolism*
D004075:Diglycerides; D010713:Phosphatidylcholines; D010767:Phosphorylcholine; D011388:Prolactin; D010738:Type C Phospholipases