Muramylpeptides augment expression of Ia-antigens on mouse macrophages.
The muramylpeptide N-acetylglucosaminyl-beta 1----4-N-acetylmuramyl-L-alanyl-D-isoglutamine (GMDP) was shown by flow cytometry to produce a dose-dependent increase in the expression of Ia-antigens by mouse peritoneal macrophages in vitro. Muramyldipeptide (N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP) had a similar, but weaker, effect. GMDP also induced expression of Ia-antigens by murine peritoneal macrophages in vivo. GMDP acted directly on the macrophages because Ia-antigen expression by cells of the cloned mouse myelomonocytic line WEHI-3 was also induced. Expression of the interleukin-2 receptor on the surface of the macrophages was also stimulated by GMDP, indicating that GMDP may influence development of the immune response through this mechanism.
Nesmeyanov
V A
VA
Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Moscow.
Khaidukov
S V
SV
Komaleva
R L
RL
Andronova
T M
TM
Ivanov
V T
VT
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D000276:Adjuvants, Immunologic; D000818:Animals; D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D005434:Flow Cytometry; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis*; D007700:Kinetics; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis
D000276:Adjuvants, Immunologic; D000949:Histocompatibility Antigens Class II; D015375:Receptors, Interleukin-2; D000119:Acetylmuramyl-Alanyl-Isoglutamine; C023868:N-acetyl-beta-glucosaminyl-N-acetylmuramyl-alanylisoglutamine