Detection of antigalactosyltransferase antibodies in plasma from patients with antisperm antibodies.
Sperm-associated galactosyltransferase has been implicated as a macromolecule involved in sperm to egg binding interactions during fertilization. An analysis of human plasma from 18 patients with antibodies to sperm antigens detected antigalactosyltransferase antibodies when compared with 10 control patients. These plasma antibodies were all able to immunoprecipitate enzyme activity from a preparation of human milk galactosyltransferase but varied in their ability to detect the protein by Western blot analyses. These results confirm the antigenic presence of galactosyltransferase in human sperm.
Humphreys-Beher
M G
MG
University of Florida, Gainesville.
Garrison
P W
PW
Blackwell
R E
RE
D000906:Antibodies / Q000097:blood*; D000941:Antigens / Q000276:immunology; D015153:Blotting, Western; D005700:Galactosyltransferases / Q000276:immunology*; D006801:Humans; D007163:Immunosorbent Techniques; D008297:Male; D013094:Spermatozoa / Q000201:enzymology / Q000276:immunology*
D000906:Antibodies; D000941:Antigens; D005700:Galactosyltransferases