Canine pancreatic islet transplantation: a comparison of two isolation techniques.
A new method (type B) for isolation of canine islets was developed, utilizing intraductal collagenase perfusion, stationary digestion, filtration and tissue separation by means of a dextran density gradient. The results of this technique were compared with those of a previously established method (type A). Islets were autotransplanted either via the splenic or the portal vein. Lasting normoglycemia was obtained in 5/8 intrasplenic type B transplants (63%), 6/8 intrahepatic type B transplants (75%), 5/6 intrasplenic type A transplants (83%) and 0/3 intrahepatic type A transplants. No difference was found in metabolic parameters 2 weeks after successful transplantation following type A or B islet isolation. Islet yield was higher with type A and purification better with type B isolation. Both techniques are relatively simple and inexpensive. Because of its higher purification rate and the success of intrahepatic islet transplantation method B has replaced method A as the routine procedure in our laboratory.
van der Vliet
J A
JA
Department of Surgery, University of Minnesota, Minneapolis.
Kaufman
D B
DB
Meloche
R M
RM
van der Hem
L G
LG
Field
M J
MJ
Sutherland
D E
DE
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D004285:Dogs; D005069:Evaluation Studies as Topic; D005215:Fasting; D005260:Female; D005951:Glucose Tolerance Test; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000378:metabolism; D016381:Islets of Langerhans Transplantation; D008297:Male; D011184:Postoperative Period; D009927:Tissue and Organ Procurement / Q000379:methods*; D014182:Transplantation, Autologous
D001786:Blood Glucose; D007328:Insulin