[Evaluation of methods for the detection of syncytial respiratory virus in nasopharyngeal secretions].
The screening for respiratory syncytial virus (RSV) in nasopharyngeal secretions with enzyme immunoassay (ELISA) and indirect immunofluorescence (IIF) has been evaluated in infants and young children with acute respiratory infection. Both methods were compared with viral isolation in HEp-2 cells and the investigation of fluorescent foci in cell cultures inoculated by centrifugation. 226 samples were evaluated by IFF, 182 of which were also evaluated by ELISA while 158 were inoculated into cell cultures. 20.35% of samples were positive with IFF and 19.23% with ELISA. Isolation of RSV was obtained in 25 of the samples inoculated into HEp-2 cells (15.8%). The cytopathic effect took a mean of 5.4 days to develop. The investigation of fluorescent foci in centrifugated cultures allowed to detect 76% of positive samples 24 hours after centrifugation and 84% of positive samples 48 hours after it. Considering the viral isolation as the reference method, IIF and ELISA had a 88% and 76% sensitivity, respectively, with very similar specifities (90.2% and 91.7%).
Buesa
F J
FJ
Departamento de Microbiología, Facultad de Medicina, Universidad de Valencia.
García-Verdú
R
R
Pastor
M
M
Escribano
A
A
D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D009093:Mucus / Q000382:microbiology; D009305:Nasopharynx / Q000378:metabolism / Q000382:microbiology; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification*; D010253:Respirovirus Infections / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D013997:Time Factors
Valoración de métodos de detección de virus respiratorio sincitial en secreciones nasofaríngeas.