Identification of nucleohistones by glycosylation and basic dyes.
Earlier examinations have shown that reducing sugars mainly react with the epsilon-amino group of lysine. After DNA extraction, lysine-rich histone proteins of alcohol fixed nuclei of rat organs were treated with glucose-6-phosphate. It was possible to selectively stain the histone type proteins of DNA negative nuclei with basic dyes.
Jobst
K
K
Department of Clinical Chemistry and Pathology, Medical University, Pécs, Hungary.
Lakatos
A
A
Horváth
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D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry*; D004396:Coloring Agents; D005947:Glucose / Q000378:metabolism; D005958:Glucosephosphates; D006651:Histocytochemistry; D006657:Histones / Q000032:analysis*; D007525:Isoelectric Focusing; D007668:Kidney / Q000648:ultrastructure; D008099:Liver / Q000648:ultrastructure; D008239:Lysine / Q000032:analysis; D010710:Phosphates / Q000032:analysis; D051381:Rats
D004396:Coloring Agents; D005958:Glucosephosphates; D006657:Histones; D010710:Phosphates; D005947:Glucose; D008239:Lysine