title: Involvement of external calcium in the release of arachidonic acid by mouse peritoneal macrophages.
abstract: The present investigation was undertaken to study the potential role of extracellular calcium on the release of arachidonic acid from mouse peritoneal macrophages. Both in phorbol ester-treated and in Ca2(+)-depleted cells, a rapid release of arachidonic acid was seen in direct response to added Ca2+. The response was directly dependent on the extracellular Ca2+ concentration, with a Ca2+ threshold of 100 nM. These results support the notion that arachidonic acid release in macrophages is functionally coupled to influx of external calcium.
authors.affiliation: Centro de Investigación, Hospital 12 de Octubre, Madrid, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Fernández
authors.forename: J
authors.initials: J
authors.lastname: Balsinde
mesh_terms: D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000502:physiology*; D005110:Extracellular Space; D005260:Female; D066298:In Vitro Techniques; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D001095:Arachidonic Acids; D016718:Arachidonic Acid; D000001:Calcimycin; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium