Cloning a gene coding for norflurazon resistance in cyanobacteria.
The herbicide norflurazon inhibits carotene biosynthesis in photosynthetic organisms by blocking the enzyme phytoene dehydrogenase (= phytoene desaturase). We have isolated norflurazon-resistant mutants of the cyanobacterium Synechococcus PCC7942. The herbicide-resistance gene from the mutant NFZ4 has been cloned by genetic complementation of the resistance trait in wild type cells. The experiment described here illustrates the usefulness of employing cyanobacteria to clone herbicide-resistance genes in a quick and simple way.
Chamovitz
D
D
Department of Genetics, Hebrew University of Jerusalem, Israel.
Pecker
I
I
Sandmann
G
G
Böger
P
P
Hirschberg
J
J
D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D004351:Drug Resistance / Q000235:genetics; D015723:Gene Library; D017343:Genes, Plant; D005816:Genetic Complementation Test; D006540:Herbicides / Q000494:pharmacology*; D009154:Mutation; D010088:Oxidoreductases / Q000235:genetics; D010957:Plasmids; D011724:Pyridazines / Q000494:pharmacology*; D015183:Restriction Mapping
D006540:Herbicides; D011724:Pyridazines; D010088:Oxidoreductases; C022398:phytoene dehydrogenase; C008677:norflurazone