The amino acid sequence of chymopapain from Carica papaya.
Chymopapain is a polypeptide of 218 amino acid residues. It has considerable structural similarity with papain and papaya proteinase omega, including conservation of the catalytic site and of the disulphide bonding. Chymopapain is like papaya proteinase omega in carrying four extra residues between papain positions 168 and 169, but differs from both papaya proteinases in the composition of its S2 subsite, as well as in having a second thiol group, Cys-117. Some evidence for the amino acid sequence of chymopapain has been deposited as Supplementary Publication SUP 50153 (12 pages) at the British Library Document Supply Centre, Boston Spa., Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies may be obtained on the terms indicated in Biochem. J. (1990) 265, 5. The information comprises Supplement Tables 1-4, which contain, in order, amino acid compositions of peptides from tryptic, peptic, CNBr and mild acid cleavages, Supplement Fig. 1, showing re-fractionation of selected peaks from Fig. 2 of the main paper. Supplement Fig. 2, showing cation-exchange chromatography of the earliest-eluted peak of Fig. 3 of the main paper, Supplement Fig. 3, showing reverse-phase h.p.l.c. of the later-eluted peak from Fig. 3 of the main paper, and Supplement Fig. 4, showing the separation of peptides after mild acid hydrolysis of CNBr-cleavage fragment CB3.
Watson
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Division of Biological Sciences, National Research Council of Canada, Ottawa, Ont.
Yaguchi
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Lynn
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D000595:Amino Acid Sequence; D001665:Binding Sites; D002917:Chymopapain; D003488:Cyanogen Bromide; D003546:Cysteine Endopeptidases; D004220:Disulfides / Q000378:metabolism; D008969:Molecular Sequence Data; D010206:Papain; D010434:Pepsin A; D010446:Peptide Fragments / Q000302:isolation & purification; D010940:Plant Proteins; D010944:Plants / Q000201:enzymology*; D014357:Trypsin
D004220:Disulfides; D010446:Peptide Fragments; D010940:Plant Proteins; D014357:Trypsin; D003546:Cysteine Endopeptidases; D010206:Papain; C039974:caricain; D002917:Chymopapain; D010434:Pepsin A; D003488:Cyanogen Bromide