The human Evi-1 gene is located on chromosome 3q24-q28 but is not rearranged in three cases of acute nonlymphocytic leukemias containing t(3;5)(q25;q34) translocations.
The murine Evi-1 gene encodes a protein that has multiple 28-amino acid repeats containing the consensus sequence found in the zinc finger domains of many transcriptional regulatory proteins. Activation of the expression of the Evi-1 gene is frequently found in murine myeloid leukemias and leukemia cell lines and is due to retroviral insertions in the 5' region of the gene in either the Evi-1 or the CB-1/FIM3 common sites of viral integrations. To examine the role of the Evi-1 gene in human leukemias we have cloned regions of the human locus corresponding to the coding region of the gene and regions corresponding to the Evi-1 and CB-1/FIM3 common sites of integrations. Using these probes we demonstrate that the human Evi-1 gene maps to chromosome 3q24-q28 in a region that is translocated in acute nonlymphocytic leukemias with a t(3;5)(q25;q34). By in situ hybridization with metaphase chromosomes from one patient with a 3;5 translocation, the Evi-1 gene was found to be translocated to the derivative 5 chromosome. However, no rearrangements were detected by Southern blot analysis with DNAs from three patients with a t(3;5) using probes from the Evi-1 or CB-1/FIM3 loci. No Evi-1 transcripts were detected with RNA from leukemic blasts of one patient with a t(3;5).
Morishita
K
K
Department of Biochemistry, St. Jude Children's Research Hospital, Memphis, Tennessee 38101.
Parganas
E
E
Bartholomew
C
C
Sacchi
N
N
Valentine
M B
MB
Raimondi
S C
SC
Le Beau
M M
MM
Ihle
J N
JN
D015152:Blotting, Northern; D015139:Blotting, Southern; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D015321:Gene Rearrangement; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D000074008:MDS1 and EVI1 Complex Locus Protein; D008667:Metalloproteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D011519:Proto-Oncogenes; D015183:Restriction Mapping; D014157:Transcription Factors; D014158:Transcription, Genetic; D014178:Translocation, Genetic; D015032:Zinc / Q000378:metabolism
D004268:DNA-Binding Proteins; D000074008:MDS1 and EVI1 Complex Locus Protein; C063575:MECOM protein, human; D008667:Metalloproteins; D014157:Transcription Factors; D015032:Zinc