Assignments of the iminoproton resonances of Bombyx mori tRNA(UCCGly) and the comparison of its structure and stability with those of tRNA(GCCGly).
Most of the iminoproton resonances in the 1H-NMR spectrum of Bombyx mori tRNA(UCCGly) have been assigned by the sequential NOEs. Any peak which indicates the presence of the tertiary GC base pair between the D and T loops could not be detected. The effects of temperature and the addition of magnesium ions and spermine on the 1H-NMR spectrum of this tRNA were examined. From the temperature change, it was found that the acceptor stem and the D stem in Bombyx mori tRNA(UCCGly) are equally stable even in the absence of magnesium, which is different from tRNA(GCCGly) where the D stem is not so stable.
Amano
M
M
Institute for Protein Research, Osaka University, Japan.
Kyogoku
Y
Y
Kawakami
M
M
D000818:Animals; D001483:Base Sequence; D012831:Bombyx; D003062:Codon; D008274:Magnesium / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012353:RNA, Transfer, Gly / Q000737:chemistry*; D013096:Spermine / Q000494:pharmacology; D013816:Thermodynamics
D003062:Codon; D012353:RNA, Transfer, Gly; D013096:Spermine; D008274:Magnesium