Fast-LC determination of 1-methyl-1H-tetrazole-5-thiol in human plasma with column-switching sample clean-up.
A simple, fast and sensitive method for the quantitative determination of 1-methyl-1H-tetrazole-5-thiol using HPLC is reported. Samples are deproteinated by plasma water filtration and injected into a HPLC system capable of column switching and backflushing the analytical column. The limit of quantitation was determined to be 70 ng ml-1 and the limit of detection 22 ng ml-1. Between-day precision (expressed as percent coefficient of variation) of standard curve slopes was +/- 3.5% with a range of within-day percent coefficient of variations from 0.28 to 1.4%. Recovery and precision of spiked control samples was 96 +/- 7% over a concentration range of 630-6300 ng ml-1.
Bombardt
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Biofluids Analytical Laboratories, Upjohn Company, Kalamazoo, MI 49001.
Courtney
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Ko
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D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013777:Tetrazoles / Q000097:blood*
D013777:Tetrazoles; C038625:1-N-methyl-5-thiotetrazole