Acyl-CoA: 1-acyl-glycero-3-phosphoethanolamine O-acyltransferase and liver plasma membrane fluidity.
Investigations have been carried out on the influence of membrane lipid composition and physical state on acyl-CoA: 1-acyl-glycerol-3-phosphoethanolamine O-acyltransferase activity in rat liver plasma membranes. The lipid composition of the membranes was modified either by way of lipid transfer proteins or by partial delipidation with exogenous phospholipases and subsequent enrichment of the membranes with different phospholipids. The results indicated that membrane rigidification by enrichment of the membranes with DPPC or SM reduced the transfer of oleic and palmitic acid to lysophosphatidylethanolamine, whereas all phospholipids inducing membrane fluidization lead to acyltransferase activation. The eventual role of membrane fluidity in the deacylation-reacylation cycle is discussed.
Momchilova-Pankova
A B
AB
Central Laboratory of Biophysics, Bulgarian Academy of Sciences, Sofia.
Markovska
T T
TT
Koumanov
K S
KS
D000215:Acylation; D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D008563:Membrane Lipids / Q000378:metabolism; D010741:Phospholipases A / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D008563:Membrane Lipids; C053809:1-acylglycerophosphoethanolamine acyltransferase; D000217:Acyltransferases; D010741:Phospholipases A