Production of arachidonic and linoleic acid metabolites by guinea pig tracheal epithelial cells.
Pulmonary epithelial cells may be responsible for regulating airway smooth muscle function, in part by release of fatty acid-derived mediators. Incubation of isolated guinea pig tracheal epithelial cells with radiolabeled arachidonic acid (AA) leads to the production of 5- and 15-hydroxyeicosatetraenoic acid (5- and 15-HETE) and smaller amounts of leukotriene (LT) B4 and C4 and 12-hydroxyheptadecatrienoic acid (HHT). Epithelial cells also are able to release linoleic acid (LA) metabolites. Incubation with radiolabeled linoleic acid leads to the formation of 9- and 13-hydroxyoctadecadienoic acid (9- and 13-HODE). The biological significance of these mediators produced by epithelial cells is discussed.
Oosthuizen
M J
MJ
Department of Pharmacology, Faculty of Pharmacy, State University of Utrecht, The Netherlands.
Engels
F
F
Van Esch
B
B
Henricks
P A
PA
Nijkamp
F P
FP
D000818:Animals; D000991:Antithrombins / Q000096:biosynthesis; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D002250:Carbon Radioisotopes; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D006168:Guinea Pigs; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis; D008041:Linoleic Acids / Q000096:biosynthesis / Q000378:metabolism*; D044243:Linoleic Acids, Conjugated; D008297:Male; D010477:Perfusion; D014132:Trachea / Q000378:metabolism*
D000991:Antithrombins; D001095:Arachidonic Acids; D002250:Carbon Radioisotopes; D005227:Fatty Acids; D006893:Hydroxyeicosatetraenoic Acids; D008041:Linoleic Acids; D044243:Linoleic Acids, Conjugated; C024347:9-hydroxy-10,12-octadecadienoic acid; D016718:Arachidonic Acid; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; C024348:13-hydroxy-9,11-octadecadienoic acid; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid