title: Mast cell growth-enhancing activity (MEA) is structurally related and functionally identical to the novel mouse T cell growth factor P40/TCGFIII (interleukin 9).
abstract: We have previously shown that certain bone marrow-derived mast cell (BMMC) lines proliferate in response to a mast cell growth-enhancing activity (MEA) that is distinct from interleukin (IL) 3 and IL 4. Here we provide evidence that MEA is identical with the recently cloned mouse T cell growth factor P40. The evidence is as follows: (a) recombinant P40 displayed all the biological activities ascribed to MEA: it supported the growth of MEA-sensitive BMMC lines, it induced IL 6 secretion by these cells, and it enhanced survival of primary mast cell cultures; (b) highly purified MEA stimulated the growth of P40-dependent cell lines; (c) a rabbit monospecific antiserum directed against P40 specifically inhibited the action of MEA on BMMC; (d) specific binding sites for P40 were detected on BMMC and (e) MEA competed with P40 for binding to P40-dependent T cells, indicating that the two molecules interact with the same receptor. These observations further extend the range of biological activities ascribed to P40 and warrant its proposed designation as IL9.
authors.affiliation: GSF-Institut für Experimentelle Hämatologie, Munich, FRG.
authors.forename: L
authors.initials: L
authors.lastname: Hültner
authors.forename: C
authors.initials: C
authors.lastname: Druez
authors.forename: J
authors.initials: J
authors.lastname: Moeller
authors.forename: C
authors.initials: C
authors.lastname: Uyttenhove
authors.forename: E
authors.initials: E
authors.lastname: Schmitt
authors.forename: E
authors.initials: E
authors.lastname: Rüde
authors.forename: P
authors.initials: P
authors.lastname: Dörmer
authors.forename: J
authors.initials: J
authors.lastname: Van Snick
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D001854:Bone Marrow Cells; D006133:Growth Substances / Q000502:physiology; D007106:Immune Sera; D066298:In Vitro Techniques; D016906:Interleukin-9; D007378:Interleukins / Q000502:physiology; D008407:Mast Cells / Q000502:physiology*; D051379:Mice; D013601:T-Lymphocytes / Q000502:physiology
chemical_list: D006133:Growth Substances; D007106:Immune Sera; D016906:Interleukin-9; D007378:Interleukins