title: Rabbit Ig kappa 1b6 gene structure.
abstract: Previous studies employing Southern blot analyses have detected multiple kappa-homologous sequences within EcoRI-digested DNA isolated from kappa 1b6 homozygous rabbits and kappa 1b6 L chain secreting RMH H158 cell line. These results are very unexpected because the published partial protein sequence for the kappa 1b6 C region is incompatible with an EcoRI restriction endonuclease recognition sequence at the nucleotide level for this allotype. To determine their identity, the kappa-homologous sequences were isolated from DNA extracted from a kappa 1b6 L chain secreting RMH H158 cell line by molecular cloning. Structural analyses demonstrated these sequences to contain genetic information encoding the majority of the kappa 1b6 L chain gene locus. The protein sequence deduced from the kappa 1b6 C region gene was shown to differ from the published partial kappa 1b6 C region protein sequence at five amino acid positions. One of these differences results in a glycine to serine interchange that introduces an EcoRI restriction endonuclease recognition site within the kappa 1b6 C region gene. Subsequent genomic Southern blot analyses confirmed this structural assignment. Based on these data, the EcoRI-sensitive kappa-homologous fragments present within the genomes of the RMH H158 cell line and kappa 1b6 homozygous rabbits represent the nominal kappa 1 gene and not an alternative kappa isotype or kappa pseudogene. Rabbit Ig kappa 1 allelic nucleotide sequence homology comparisons have shown the isolated kappa 1b6 J-C gene locus to display common structural features previously identified in other kappa 1 alleles.
authors.affiliation: Weis Center for Research, Geisinger Clinic, Danville, PA 17822.
authors.forename: K L
authors.initials: KL
authors.lastname: Dreher
authors.forename: V
authors.initials: V
authors.lastname: Asundi
authors.forename: B
authors.initials: B
authors.lastname: Wolf
authors.forename: L
authors.initials: L
authors.lastname: Bruggeman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D015870:Gene Expression; D005803:Genes, Immunoglobulin; D007126:Immunoglobulin Allotypes / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D008969:Molecular Sequence Data; D011817:Rabbits; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D007126:Immunoglobulin Allotypes; D007145:Immunoglobulin kappa-Chains