title: Measurement of 5-methyltetrahydrofolic acid in man by high-performance liquid chromatography.
abstract: 5-Methyl-5,6,7,8-tetrahydrofolic (5-methylTHF) acid can be measured in human serum and whole blood by fluorescence after deproteinization with perchloric acid and separation by high-performance liquid chromatography (HPLC). The technique is sensitive, rapid, and correlates with existing methods for total folates.
authors.affiliation: Haematology Department, General Hospital, Birmingham B4, England.
authors.forename: R J
authors.initials: RJ
authors.lastname: Leeming
authors.forename: A
authors.initials: A
authors.lastname: Pollock
authors.forename: L J
authors.initials: LJ
authors.lastname: Melville
authors.forename: C G
authors.initials: CG
authors.lastname: Hamon
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007202:Indicators and Reagents; D007780:Lacticaseibacillus casei / Q000187:drug effects / Q000254:growth & development; D013050:Spectrometry, Fluorescence; D013763:Tetrahydrofolates / Q000097:blood* / Q000494:pharmacology
chemical_list: D007202:Indicators and Reagents; D013763:Tetrahydrofolates; C005984:5-methyltetrahydrofolate