The establishment of rat hybridoma cell lines secreting McAb against strains of potato virus Y and analysis of its stability.
The rat splenocytes immunized with potato virus Y (PVYn) and ratmyeloma (IR983) were fused by PEG (M. W.1450). Three kinds of stable hybridoma cell lines secreting specific monoclonal antibodies (McAbs) were derived. One kind of the cell lines producing McAbs reacts to PVYn specifically. Another reacts to PVYo specifically. The third one reacts to both of the two strains. Tested by the methods of sandwich-ELISA and indirect-ELISA, all kinds of McAbs did not react to seven plant viruses: tobacco mosaic (TMV), cucumber mosaic (CMV), tobacco tech (TEV), alfalfa mosaic (AMV), turnip mosaic (TuMV), potato leaf roll (PLRV), potato virus X (PVX). The biological properties of the hybridoma cell lines and the McAbs were tested.
Guo
J
J
Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing.
Xiao
X W
XW
Cai
S H
SH
Lu
W C
WC
Liu
X P
XP
Hsu
H T
HT
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000166:cytology / Q000378:metabolism*; D010942:Plant Viruses / Q000276:immunology* / Q000302:isolation & purification; D051381:Rats; D013696:Temperature
D000911:Antibodies, Monoclonal