Regulation of a thermostable alpha-amylase of Streptomyces thermoviolaceus CUB74: maltotriose is the smallest inducer.
We have examined induction and repression by various sugars and carbon sources of the synthesis of a thermostable alpha-amylase in its natural host, S thermoviolaceus CUB74. The smallest molecule capable of inducing synthesis of the enzyme was maltotriose whereas maltose had no effect which might suggest a different control system from that found in other streptomycete amylases. Addition of mannitol to the growth medium impeded the alpha-amylase induction whereas glucose had no effect. After cloning of its gene into a new streptomycete host, S lividans TK24, the S thermoviolaceus alpha-amylase could not be induced by any of the sugars tested.
Bahri
S M
SM
Department of Biochemistry and Molecular Biology, University College London, U.K.
Ward
M
M
D004790:Enzyme Induction / Q000187:drug effects / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics; D013696:Temperature; D014312:Trisaccharides / Q000494:pharmacology; D000516:alpha-Amylases / Q000096:biosynthesis* / Q000235:genetics
D014312:Trisaccharides; C008317:maltotriose; D000516:alpha-Amylases