Dual regulation by G proteins of agonist-dependent phosphorylation of muscarinic acetylcholine receptors.
Muscarinic acetylcholine receptors purified from porcine atrium were phosphorylated, depending on the presence of agonists, by a protein kinase partially purified from porcine brain, which had similar properties to the beta-adrenergic receptor kinase. GTP-binding regulatory proteins (Go) had dual effects on the phosphorylation of muscarinic receptors, i.e. stimulation at lower concentrations and inhibition at higher concentrations. The stimulatory effect was reproduced with the beta gamma subunit of Go and the inhibitory effect with the combination of the alpha and beta gamma subunits.
Haga
K
K
Department of Biochemistry, Faculty of Medicine, University of Tokyo, Japan.
Haga
T
T
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000502:physiology*; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D010766:Phosphorylation; D011976:Receptors, Muscarinic / Q000502:physiology*; D015398:Signal Transduction; D013329:Structure-Activity Relationship; D013552:Swine
D046911:Macromolecular Substances; D011976:Receptors, Muscarinic; D001285:Atropine; D000255:Adenosine Triphosphate; D002217:Carbachol; D019204:GTP-Binding Proteins