Haematopoietic suppressing activity of gamma-interferon in serum and bone marrow of aplastic anaemia patients.
To determine the mechanism by which haematopoiesis is suppressed in aplastic anaemia, the effect of the sera from 6 patients on the granulopoietic precursors (colony-forming units in culture; CFU-C) was studied in vitro. Addition of the sera from 2 patients significantly suppressed CFU-C. The suppressive effect of the sera on CFU-C was inhibited by the addition of 1.5 NU/ml of anti-gamma-IFN antibody. In another patient, anti-gamma-IFN antibody increased autologous CFU-C although the serum of the patient did not suppress CFU-C. Serum gamma-IFN levels of all patients were under 2 IU/ml. The above findings suggest that humoral factors inhibit haematopoiesis in some patients with aplastic anaemia, and that gamma-IFN plays a role as an inhibitor even at a low concentration.
Tohda
S
S
First Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
Suzuki
T
T
Nagata
K
K
Nara
N
N
Aoki
N
N
D000293:Adolescent; D000328:Adult; D000368:Aged; D000741:Anemia, Aplastic / Q000097:blood / Q000503:physiopathology*; D001853:Bone Marrow / Q000503:physiopathology*; D001854:Bone Marrow Cells; D006410:Hematopoiesis / Q000502:physiology*; D006801:Humans; D007371:Interferon-gamma / Q000502:physiology*
D007371:Interferon-gamma