Identification of germination gene of Bacillus megaterium.
Glucose, KNO3, proline and leucine initiate the spore germination of B. megaterium ATCC 12872, but not of B. megaterium ATCC 19213. In order to isolate the gene concerning germination of B. megaterium ATCC 12872, we constructed its gene library in plasmid vector, and introduced into B. megaterium ATCC 19213. We obtained a transformant whose spores differed from those of the wild type strain with respect to germinability. Spores of this transformant could be germinated by glucose, proline or leucine. The recombinant plasmid prepared from this transformant was found to carry 2 kilobase pairs fragment of B. megaterium ATCC 12872 DNA. This fragment may contain the gene encoding the protein which plays an important role in germination.
Tani
K
K
Department of Environmental Chemistry and Microbiology, Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Kawanishi
M
M
Nishikawa
J
J
Sasaki
M
M
Takubo
Y
Y
Nishihara
T
T
Kondo
M
M
D000596:Amino Acids / Q000494:pharmacology; D001410:Bacillus megaterium / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D005798:Genes, Bacterial; D005947:Glucose / Q000494:pharmacology; D006358:Hot Temperature; D007700:Kinetics; D010957:Plasmids; D013171:Spores, Bacterial / Q000187:drug effects / Q000502:physiology; D014169:Transformation, Bacterial
D000596:Amino Acids; D005947:Glucose