The simultaneous acute release of tissue-type plasminogen activator and von Willebrand factor in the perfused rat hindleg region.
In perfused rat hindlegs, platelet-activating factor and bradykinin induced the acute release of both tissue-type plasminogen activator (t-PA) and von Willebrand Factor (vWF). The time course of release was similar for both proteins, and the amounts of t-PA and vWF released under various conditions were closely correlated. Release of both t-PA and vWF required extracellular calcium, and could be induced by the calcium ionophore A-23187. Protein synthesis was not required for release to occur. Phorbol myristate acetate also induced release of t-PA and vWF, though with a different time course; DDAVP was inactive. The results suggest that the release of t-PA, and that of vWF, are closely linked at the cellular level.
Tranquille
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Gaubius Institute TNO, Leiden, The Netherlands.
Emeis
J J
JJ
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D006614:Hindlimb / Q000098:blood supply; D008297:Male; D010477:Perfusion; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors; D010959:Tissue Plasminogen Activator / Q000378:metabolism*; D014841:von Willebrand Factor / Q000378:metabolism*
D014841:von Willebrand Factor; D000001:Calcimycin; D011493:Protein Kinase C; D010959:Tissue Plasminogen Activator; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium