Modulation of the immune response to transplanted tumors in rats by selective depletion of neutrophils in vivo using a monoclonal antibody: abrogation of specific transplantation resistance to chemical carcinogen-induced syngeneic tumors by selective depletion of neutrophils in vivo.
The role of neutrophils in transplantation immunity to syngeneic rat tumors was examined using a monoclonal antibody (RP-3) that depletes rat neutrophils selectively in vivo. We used 2 chemical carcinogen-induced transplanted tumors of different antigenic specificity (KMT-17 and KDH-8 of WKA rat origin). When neutrophils were selectively depleted by i.p. injection of RP-3 at the time of in vivo priming with X-irradiated tumor cells, the growth of subsequently s.c. transplanted identical tumors was not inhibited, in contrast to the group of rats immunized without RP-3 treatment. Tumor growth was also not inhibited when the immune rats were treated with RP-3 at the time of identical viable tumor cell challenge. These results suggest that neutrophils play a role in both the priming and effector phases of specific transplantation resistance to syngeneic tumors.
Midorikawa
Y
Y
Department of Parasitology, Yamagata University School of Medicine, Japan.
Yamashita
T
T
Sendo
F
F
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D005354:Fibrosarcoma / Q000139:chemically induced / Q000276:immunology*; D006084:Graft Rejection / Q000276:immunology; D007111:Immunity, Cellular; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000276:immunology*; D008748:Methylcholanthrene; D009368:Neoplasm Transplantation; D009504:Neutrophils / Q000276:immunology*; D051381:Rats; D014181:Transplantation Immunology
D000911:Antibodies, Monoclonal; D008748:Methylcholanthrene; D015127:9,10-Dimethyl-1,2-benzanthracene