Isolation of two hemorrhagic toxins from Crotalus basiliscus basiliscus (Mexican west coast rattlesnake) venom and their effect on blood clotting and complement.
1. Two hemorrhagic toxins of mol. wt 27,000 (B1) and 27,500 (B2) and pI 9.8 and 5.2 respectively were isolated from Crotalus basiliscus venom. 2. The two proteinases did not cross-react antigenically. 3. Both toxins caused hemorrhage in mice and each was capable of hydrolyzing hide power azure, casein, collagen and fibrin. 4. B1 hydrolyzed the A alpha, B beta and gamma chains of fibrinogen. B2 hydrolyzed the A alpha and B beta chains of fibrinogen, but not the gamma chain. 5. Both proteinases inactivated guinea pig complement.
Molina
O
O
Department of Biological Sciences, University of Texas, El Paso 79968.
Seriel
R K
RK
Martinez
M
M
Sierra
M L
ML
Varela-Ramirez
A
A
Rael
E D
ED
D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D002848:Chromatography, DEAE-Cellulose; D002863:Chromogenic Compounds / Q000494:pharmacology; D003165:Complement System Proteins / Q000187:drug effects*; D003435:Crotalid Venoms / Q000032:analysis*; D004492:Edetic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000302:isolation & purification* / Q000502:physiology; D005340:Fibrinogen / Q000378:metabolism; D005343:Fibrinolytic Agents / Q000494:pharmacology; D006470:Hemorrhage / Q000139:chemically induced*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D014118:Toxins, Biological / Q000302:isolation & purification* / Q000494:pharmacology
D002863:Chromogenic Compounds; D003435:Crotalid Venoms; D005343:Fibrinolytic Agents; D014118:Toxins, Biological; D005340:Fibrinogen; D003165:Complement System Proteins; D004492:Edetic Acid; C019724:arginine esterase; D002265:Carboxylic Ester Hydrolases; D010450:Endopeptidases