title: Evaluation of enzyme-linked immunosorbent and alternative assays for detection of HIV antibodies using panels of Brazilian sera.
abstract: Sera from 472 Brazilian subjects, confirmed to be either positive or negative for HIV antibodies and comprising the total clinical spectrum of HIV infection, were utilized in the evaluation of six commercially available enzyme-linked immunosorbent assays (ELISA), as well as of four alternative assays, namely indirect immunofluorescence (IIF), passive hemagglutination (PHA), dot blot and Karpas AIDS cell test. The sensitivities ranged from 100% (Abbott and Roche ELISA) to 84.2% (PHA) and the specificities ranged from 99.3% (IIF) to 80.2% (PHA). The sensitivity and specificity of the PHA and the sensitivity of the Karpas cell test were significantly lower than those of the other tests. Although the IFF and dot blot had good sensitivities and specificities, the six ELISA were more attractive than those tests when other parameters such as ease of reading and duration of assay were considered.
authors.affiliation: Fundaçao Oswaldo Cruz, WHO Collaborating Centre on AIDS, Bahia, Brazil.
authors.forename: J
authors.initials: J
authors.lastname: Ivo-Dos-Santos
authors.forename: D L
authors.initials: DL
authors.lastname: Mello
authors.forename: J C
authors.initials: JC
authors.lastname: Couto-Fernandez
authors.forename: R M
authors.initials: RM
authors.lastname: Passos
authors.forename: L A
authors.initials: LA
authors.lastname: Dias-Carneiro
authors.forename: E A
authors.initials: EA
authors.lastname: Castilho
authors.forename: B
authors.initials: B
authors.lastname: Galvão-Castro
mesh_terms: D015153:Blotting, Western; D001938:Brazil; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D015483:HIV Antibodies / Q000032:analysis*; D006386:Hemagglutination Tests; D006801:Humans; D012680:Sensitivity and Specificity
chemical_list: D015483:HIV Antibodies