Intraocular in vivo immunofluorescence. A new technique for visualizing structures of the ocular fundus.
A new noninvasive technique for observing structures in the living eye has been developed: the technique is based on intravenous application of specific antibodies, labeled with fluorescein. The immunologically marked substructures of the fundus of the eye, the only transparent organ in mammals, are photographed directly using high speed film. Combined with a digital image processor to enhance low contrast contours, this technique can be used for diagnostic purposes, as shown in rabbits with experimental toxoplasmic chorioretinitis.
Scheiffarth
O F
OF
Eye Hospital of the University of Munich, Federal Republic of Germany.
Zrenner
E
E
Disko
R
R
Stefani
F H
FH
Brabänder
B
B
D000818:Animals; D000913:Antibodies, Protozoan; D000918:Antibody Specificity; D002825:Chorioretinitis / Q000175:diagnosis / Q000209:etiology; D005451:Fluorescein Angiography / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D005654:Fundus Oculi; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007074:Immunoglobulin G / Q000276:immunology; D010781:Photography; D011817:Rabbits; D013861:Thiocyanates; D014124:Toxoplasmosis, Animal / Q000150:complications / Q000175:diagnosis; D014126:Toxoplasmosis, Ocular / Q000150:complications / Q000175:diagnosis
D000913:Antibodies, Protozoan; D005452:Fluoresceins; D007074:Immunoglobulin G; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate