title: The effect of high doses of recombinant human erythropoietin on megakaryocytopoiesis and platelet production in splenectomized mice.
abstract: The effects of recombinant human erythropoietin (rHuEpo) on megakaryocytopoiesis and platelet production were investigated in splenectomized, sham-operated and intact mice. When splenectomized mice were injected with 50 U of rHuEpo daily for 4 d, peripheral platelet counts, megakaryocyte (MK) size and the percentage of mature MK (stage IV MK) increased from 1 to 4 d after the initial rHuEpo injection. The total number of marrow MK colony forming units (CFU-MK) increased from 2 to 4 d after the initial injection. Furthermore, from days 6 to 8, the total number of marrow MK also increased. In addition, when the dose of rHuEpo was increased to 20-200 U per mouse, clear dose responses were detected in platelet counts and MK numbers. When sham-operated and intact mice were injected with rHuEpo, no significant change in platelet numbers was detected. The number of MK, MK size and the number of CFU-MK increased only in spleen, not in bone marrow. Our data indicate that Epo has stimulatory effects on platelet production in vivo, if used in sufficient quantities.
authors.affiliation: First Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Tsukada
authors.forename: M
authors.initials: M
authors.lastname: Misago
authors.forename: M
authors.initials: M
authors.lastname: Kikuchi
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: R
authors.initials: R
authors.lastname: Ogawa
authors.forename: S
authors.initials: S
authors.lastname: Oda
authors.forename: S
authors.initials: S
authors.lastname: Chiba
authors.forename: S
authors.initials: S
authors.lastname: Eto
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D003114:Colony-Forming Units Assay; D004921:Erythropoietin / Q000235:genetics / Q000494:pharmacology*; D005260:Female; D006400:Hematocrit; D006410:Hematopoiesis / Q000187:drug effects*; D006801:Humans; D007501:Iron / Q000097:blood; D007700:Kinetics; D008533:Megakaryocytes / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D010976:Platelet Count / Q000187:drug effects; D011994:Recombinant Proteins / Q000494:pharmacology; D012016:Reference Values; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013156:Splenectomy; D013997:Time Factors
chemical_list: D011994:Recombinant Proteins; D004921:Erythropoietin; D007501:Iron