title: Histones H1 and H4 are present near the replication fork.
abstract: The presence of histones H1 and H4 at the sites of actual DNA synthesis has been studied with Ehrlich ascites tumour cells, pulse labeled for different times with 3H-thymidine and then treated with formaldehyde to crosslink histones to DNA. The fixed chromatin fragments were sonicated to reduce the size of DNA, purified in a CsCl gradient and immunoprecipitated with antibodies to histones H1 and H4. Determination of specific radioactivity in precipitated probes showed that both histones have been associated with nascent DNA even upon 1 min pulse with 3H-thymidine, thus indicating their presence near the replication fork.
authors.affiliation: Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
authors.forename: V
authors.initials: V
authors.lastname: Stefanovsky
authors.forename: S
authors.initials: S
authors.lastname: Dimitrov
authors.forename: V
authors.initials: V
authors.lastname: Russanova
authors.forename: I
authors.initials: I
authors.lastname: Pashev
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D004261:DNA Replication / Q000502:physiology*; D005557:Formaldehyde; D006657:Histones / Q000302:isolation & purification*; D009707:Nucleosomes / Q000737:chemistry*; D011233:Precipitin Tests; D013936:Thymidine
chemical_list: D006657:Histones; D009707:Nucleosomes; D005557:Formaldehyde; D013936:Thymidine