title: A ferredoxin-type iron-sulfur protein gene, frx B, is expressed in the chloroplasts of tobacco and spinach.
abstract: Previously, a ferredoxin-type iron-sulfur protein, frx B protein, was identified in a high-salt extract of the purified thylakoid membrane of Chlamydomonas reinhardtii, a unicellular green alga. Polyclonal antibody was raised against a synthetic pentadecameric peptide with an amino acid sequence corresponding to the highly conserved region of the putative frx B proteins of 3 land plants. In this report, protein(s) reacting strongly and specifically with this antibody was detected in the equivalent high-salt extract prepared from purified chloroplast of spinach and tobacco. One strong reaction polypeptide band from tobacco chloroplast was purified from SDS-polyacrylamide gel and subjected to endoproteinase lys C digestion. The resulting polypeptides were separated by reversed-phase chromatography. N-terminal sequencing of 3 purified polypeptides revealed that the protein is encoded by the 'frxB gene' identified from DNA sequence analysis.
authors.affiliation: Institute of Molecular Biology, Academia Sinica, Nankang, Taipei, Republic of China.
authors.forename: C H
authors.initials: CH
authors.lastname: Lin
authors.forename: M
authors.initials: M
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D002736:Chloroplasts / Q000378:metabolism*; D017343:Genes, Plant; D007425:Intracellular Membranes / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D010944:Plants / Q000235:genetics*; D010947:Plants, Toxic; D014026:Nicotiana / Q000235:genetics*
chemical_list: D007506:Iron-Sulfur Proteins; D010940:Plant Proteins; C070793:frx B protein, Chlamydomonas reinhardtii