Myristoylated src-oncogene products are potently detected by the monoclonal antibody to the NH2-terminal myristoyl-Gly-Ser-Ser-Lys src-peptide.
Monoclonal antibodies were raised against a synthetic NH2-terminal myristoyl tetrapeptide (N-myristoyl-Gly-Ser-Ser-Lys) which is characteristic of an NH2-terminal portion of pp60src, the transforming protein of src-oncogene. The antibody reacted with the albumin conjugated with both the N-myristoyl and N-lauroyl-tetrapeptides, but concentrations at which 50% of the immunoreaction was inhibited were 5 pmol for the N-myristoyl and 830 pmol for N-lauroyl tetrapeptidyl albumin. On the other hand, N-palmitoyl tetrapeptidyl and underivatized albumin, and Gly-Ser-Ser-Lys-Ser-Lys-Pro-Lys octapeptide had no effects. These results suggest a high affinity of the antibody for an N-myristoyl-Gly-Ser-Ser-Lys moiety. src-Oncogene products in Rous sarcoma virus-transformed cells and human colon carcinoma tumor cells were selectively identified as myristoylated pp60src by immunoprecipitation analyses with the antibody.
Shoji
S
S
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
Kida
Y
Y
Takenaka
O
O
Yoshinaga
T
T
Funakoshi
T
T
Kubota
Y
Y
D000230:Adenocarcinoma / Q000276:immunology; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D003110:Colonic Neoplasms / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D009101:Multiple Myeloma / Q000276:immunology; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism*; D009842:Oligopeptides / Q000276:immunology*; D015688:Oncogene Protein pp60(v-src) / Q000276:immunology*; D010190:Pancreatic Neoplasms / Q000276:immunology; D010455:Peptides / Q000276:immunology*; D011233:Precipitin Tests; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D009227:Myristic Acids; D009842:Oligopeptides; D010455:Peptides; D019814:Myristic Acid; C065177:myristoyl-glycyl-seryl-seryl-lysine; D015688:Oncogene Protein pp60(v-src)