title: An ELISA for the serodiagnosis of tuberculosis using a 30,000-Da native antigen of Mycobacterium tuberculosis.
abstract: An ELISA was established for the measurement of IgG antibody in human serum to the 30,000-Da native antigen of Mycobacterium tuberculosis and evaluated for its utility in the diagnosis of tuberculosis at the Instituto Nacional de Enfermedades Respiratorias in Mexico City. The test had a sensitivity of 70% in patients with sputum-positive active pulmonary tuberculosis and a specificity in control subjects of 100%. The accuracy of positive prediction was 100% and of negative prediction 93% for patients with pulmonary tuberculosis at this institute. Less favorable test characteristics were obtained for patients with miliary and pleural tuberculosis, in which the test had sensitivities of 22% and 14%. These results offer the promise of an accurate serodiagnostic test for pulmonary tuberculosis using readily obtained reagents.
authors.affiliation: Department of Microbiology, Instituto Nacional de Enfermedades Respiratorias, Mexico, DF.
authors.forename: E
authors.initials: E
authors.lastname: Sada
authors.forename: L E
authors.initials: LE
authors.lastname: Ferguson
authors.forename: T M
authors.initials: TM
authors.lastname: Daniel
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008172:Lung Diseases, Fungal / Q000175:diagnosis; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011237:Predictive Value of Tests; D014376:Tuberculosis / Q000175:diagnosis*; D014391:Tuberculosis, Miliary / Q000175:diagnosis; D014396:Tuberculosis, Pleural / Q000175:diagnosis; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial