[The genetic transfer, heredity and phenotypic expression of plasmid RP4::Mu cts genes in Bacillus cereus].
The transcipients were obtained in intrageneric matings of E.coli donor harbouring the plasmid PR4::Mu cts 62 with Bac. cereus GP7 recipient cells with the frequency 10(-9). The transcipient clone Bac. cereus 682 was selected having stably inherited and expressed the hybrid plasmid PR4::Mu cts 62 genes for antibiotic resistance and temperature sensitivity. Production of the bacteriophage Mu cts 62 particles was not registered in the bacillary transcipient cells. The plasmid RP4::Mu cts 62 genes were localized in the chromosome of Bac. cereus 682 transcipient by the blot-hybridization technique with 32P-labelled DNA of the bacteriophage Mu cts 62 and the plasmid PR4. The transcipient of Bac. cereus 682 has the donor properties and transfers the RP4::Mu cts 62 genes to recipient cells of Bac. cereus DSM 318 with the frequency of 10(-6)-10(-7). The expression and transfer of the gram-negative plasmid genes in gram-positive bacterial cells are discussed.
Timakova
N V
NV
Aleshkin
G I
GI
Titova
I V
IV
Goderdzishvili
M G
MG
Zakharenko
V I
VI
Brukhanskiĭ
G V
GV
Loginova
O G
OG
Ditiatkin
S Ia
SIa
Il'iashenko
B N
BN
D001409:Bacillus cereus / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D005819:Genetic Markers; D010641:Phenotype; D010957:Plasmids / Q000235:genetics*; D013045:Species Specificity; D014169:Transformation, Bacterial
D005819:Genetic Markers
Geneticheskiĭ perenos, nasledovanie i fenotipicheskoe vyrazhenie genov plazmidy RP4::Mu cts v Bacillus cereus.