title: Identification and cDNA cloning of a new nodule-specific gene, Nms-25 (nodulin-25) of Medicago sativa.
abstract: A new nodule-specific gene, Nms-25 (nodulin-25), was identified in cDNA clones isolated from a nodule-specific cDNA library of Medicago sativa. The first transcript of this gene appeared 9 days after inoculation of the roots with Rhizobium meliloti. The time of expression and the quantity of the transcripts of the Nms-25 gene was similar to that of leghemoglobin genes suggesting a similar regulation. A protein of 246 amino acids could be deduced from a full-length cDNA clone. The first 24 amino acids at the N-terminal end of this protein formed a signal sequence which might direct membrane transport into the peribacteroid space. Using different predictive methods the signal sequence cleaved protein was tentatively predicted to be a water-soluble enzyme, but not hydrolase.
authors.affiliation: Institute of Genetics, Hungarian Academy of Sciences, Szeged.
authors.forename: G B
authors.initials: GB
authors.lastname: Kiss
authors.forename: E
authors.initials: E
authors.lastname: Vincze
authors.forename: Z
authors.initials: Z
authors.lastname: Végh
authors.forename: G
authors.initials: G
authors.lastname: Tóth
authors.forename: J
authors.initials: J
authors.lastname: Soós
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D015870:Gene Expression; D017343:Genes, Plant; D000455:Medicago sativa / Q000235:genetics*; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics; D015183:Restriction Mapping
chemical_list: D010940:Plant Proteins; D004247:DNA