title: Amino acid requirements for growth of Chlamydia pneumoniae in cell cultures: growth enhancement by lysine or methionine depletion.
abstract: Amino acid requirements for growth of two isolates of Chlamydia pneumoniae were studied and compared with those for one strain of Chlamydia trachomatis in a HeLa 229 cell culture. It was shown that among 13 amino acids in Eagle minimum essential medium, C. pneumoniae required all amino acids except lysine. A true requirement for arginine, isoleucine, leucine, threonine, and valine could not be determined because depletion of 100% of these amino acids caused cell detachment. Consequently, a requirement for these amino acids was based on 90% depletion. C. trachomatis biovar trachoma required all amino acids except threonine, which was indeterminable. Depletion of 100 and 90% of the lysine and 90 to 70% of the methionine was shown to enhance the growth of C. pneumoniae. This phenomenon was shown to be a property of C. pneumoniae because the effect of lysine and methionine reduction was also demonstrated in another human line, HL cells, and a mouse line, McCoy cells.
authors.affiliation: Department of Pathobiology, University of Washington, Seattle 98195.
authors.forename: C C
authors.initials: CC
authors.lastname: Kuo
authors.forename: J T
authors.initials: JT
authors.lastname: Grayston
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D002689:Chlamydia / Q000254:growth & development* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008239:Lysine / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism*; D051379:Mice
chemical_list: D000596:Amino Acids; D008715:Methionine; D008239:Lysine