Structural studies on allergen RC-13 from Ricinus communis L.: isolation and characterization of a major glycopeptide.
A glycoprotein, RC-13, isolated from Ricinus communis seeds was reduced, S-alkylated and cleaved by trypsin. The tryptic digest was fractionated by ion-exchange chromatography and a glycopeptide was isolated and purified by high-voltage paper electrophoresis. When submitted to amino acid and carbohydrate analyses this major glycopeptide showed the following chemical composition: Lys1, Asp1, Thr2, Ser4, Glu1, Pro2, Gly2, Ala2, Val2, GlcN6, Man6 and Gal8. Hydrazynolysis positioned Ser as the C-terminal residue. It is postulated that this glycopeptide belongs to the C-terminal region of the allergen.
Silva Júnior
J G
JG
Departamento de Bioquímica, Universidade Federal do Rio de Janeiro.
Garcia
M A
MA
Machado
O L
OL
Grassiano
D M
DM
Domont
G B
GB
D000485:Allergens / Q000302:isolation & purification*; D000596:Amino Acids / Q000032:analysis; D002367:Ricinus communis / Q000032:analysis*; D006020:Glycopeptides / Q000302:isolation & purification*; D010449:Peptide Mapping; D010947:Plants, Toxic; D012639:Seeds / Q000032:analysis
D000485:Allergens; D000596:Amino Acids; D006020:Glycopeptides