V kappa gene usage, idiotype expression, and antigen binding among clones expressing the VHX24 gene family derived from naive and anti-idiotype immune BALB/c mice.
The BALB/c myeloma protein ABPC48 binds beta(2-6)-linked fructosans and expresses genes derived from the VHX24 and V kappa 10 gene families. We have selected 30 hybridomas expressing the VHX24 gene family derived from mitogen-stimulated spleen cells of naive BALB/c mice and mice injected at birth with the syngeneic monoclonal anti-ABPC48Id, IDA10. The majority of mAb with kappa L chains uses V kappa 1. Antibodies reacting with IDA10 use both V kappa 10 and V kappa 1. Most of these VHX24+ mAb reacted with one or more members of a limited panel of predominantly polysaccharide Ag that have been previously observed to interact with antibodies expressing the VHX24 gene family. Nucleotide sequencing of selected VH and V kappa genes shows a very low frequency of somatic mutation. The effect of neonatal anti-Id injection on VHX24-V kappa pairing and Id expression is discussed.
Bonilla
F A
FA
Department of Microbiology, Mount Sinai School of Medicine, City University of New York, NY 10029.
Zaghouani
H
H
Rubin
M
M
Bona
C
C
D000818:Animals; D000906:Antibodies / Q000235:genetics* / Q000276:immunology; D000941:Antigens / Q000276:immunology; D001483:Base Sequence; D015870:Gene Expression; D005803:Genes, Immunoglobulin; D006825:Hybridomas; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007130:Immunoglobulin Idiotypes / Q000235:genetics*; D007132:Immunoglobulin Isotypes / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data
D000906:Antibodies; D000941:Antigens; D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes; D007132:Immunoglobulin Isotypes; D007145:Immunoglobulin kappa-Chains; D008070:Lipopolysaccharides