title: Inhibitory effects of gold sodium thiomalate on the proliferation and interferon-gamma induced HLA-DR expression in human endothelial cells.
abstract: Our study was undertaken to investigate the effects of gold sodium thiomalate (GSTM) on the proliferation and HLA-DR antigen expression of human umbilical vein endothelial cells (EC). The proliferation of the EC was determined by 3H-thymidine incorporation into the EC. The expression of HLA-DR antigen on the surface of the EC was detected by an indirect immunofluorescent method using a fluorescent flow cytometer. When GSTM was added at the start of the EC culture, GSTM at a low concentration could inhibit the proliferative response of the EC to endothelial cell growth supplement. Furthermore, when the EC were cultured with recombinant interferon-gamma (rIFN-gamma) and GSTM, GSTM was also able to suppress the HLA-DR antigen expression on the surface of EC induced by rIFN-gamma. On the time-kinetic study of the effects of GSTM on the HLA-DR antigen expression induced by rIFN-gamma, the pretreatment of GSTM was able to suppress the HLA-DR antigen expression, whereas GSTM did not affect the HLA-DR antigen expression already induced by rIFN-gamma. Our findings suggest that the therapeutic effects of gold compounds in patients with rheumatoid arthritis may be attributed to the interference of the proliferation and HLA-DR antigen expression of the EC.
authors.affiliation: First Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Kawakami
authors.forename: K
authors.initials: K
authors.lastname: Eguchi
authors.forename: K
authors.initials: K
authors.lastname: Migita
authors.forename: H
authors.initials: H
authors.lastname: Nakao
authors.forename: T
authors.initials: T
authors.lastname: Otsubo
authors.forename: Y
authors.initials: Y
authors.lastname: Ueki
authors.forename: C
authors.initials: C
authors.lastname: Shimomura
authors.forename: H
authors.initials: H
authors.lastname: Tezuka
authors.forename: M
authors.initials: M
authors.lastname: Matsunaga
authors.forename: T
authors.initials: T
authors.lastname: Ishimaru
mesh_terms: D000954:Antigens, Surface / Q000032:analysis; D002455:Cell Division / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology* / Q000276:immunology; D006052:Gold Sodium Thiomalate / Q000494:pharmacology*; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*
chemical_list: D000954:Antigens, Surface; D006684:HLA-DR Antigens; D006052:Gold Sodium Thiomalate; D007371:Interferon-gamma