Development of an enzyme-linked immunosorbent assay to detect serum antibody to chicken anemia agent.
An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to chicken anemia agent (CAA) has been developed. This test utilizes a CAA-specific mouse monoclonal antibody to selectively capture virus antigen. Chicken antibodies to CAA bind to the captured antigen and are detected with horseradish peroxidase-labeled anti-chicken immunoglobulin using a conventional indirect ELISA protocol. When 388 chicken sera from specific-pathogen-free and commercial flocks from the United Kingdom, West Germany, the United States and Australia were examined, 98.5% agreement was obtained between the results of the ELISA and the indirect immunofluorescence assay. This ELISA should have worldwide application in testing SPF and commercial chicken flocks for CAA antibodies.
Todd
D
D
Veterinary Research Laboratories, Stormont, Belfast, Northern Ireland.
Mackie
D P
DP
Mawhinney
K A
KA
Connor
T J
TJ
McNeilly
F
F
McNulty
M S
MS
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000097:blood*; D002645:Chickens / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D007106:Immune Sera / Q000276:immunology; D009500:Neutralization Tests; D014780:Viruses / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007106:Immune Sera