title: [Structural-functional organization of the incompatibility group P plasmid pBS221].
abstract: Plasmid pBS221 was physically mapped for restriction endonucleases EcoRI, BamHI, BglII, HindIII. The regions essential for the plasmid existence and participating in replication (oriV trfA*) and mobilization (mob) were cloned. The tet determinant and oriV trfA* regions were localized on the physical map of the plasmid. A DNA sequence homologous to genes of Tn501 mer operon was detected in this plasmid. The studies on homology of plasmids RP4 (IncP alpha), R751 (IncP beta) and pBS221 plasmid suggest that the latter belongs to the IncP beta subgroup.
authors.forename: O V
authors.initials: OV
authors.lastname: Parfenova
authors.forename: L A
authors.initials: LA
authors.lastname: Anisimova
authors.forename: A M
authors.initials: AM
authors.lastname: Boronin
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics; D011995:Recombination, Genetic; D012093:Replicon; D015183:Restriction Mapping; D012231:Rhizobium / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004269:DNA, Bacterial
vernacular_title: Strukturno-funktsional'naia organizatsiia plazmidy pBS221 gruppy nesovmestimosti P.