Mutagenesis of the NH2-terminal domain of elongation factor Tu.
Mutagenesis was carried out in the N-terminal domain of elongation factor Tu (EF-Tu) to characterize the structure-function relationships of this model GTP binding protein with respect to stability, the interaction with GTP and GDP, and the catalytic activity. The substitutions were introduced in elements around the guanine nucleotide binding site or in the loops defining this site, in the intact molecule or in the isolated N-terminal domain (G domain). The double substitution Val88----Asp and Leu121----Lys, two residues situated on two vicinal alpha-helices, influences the basic activities of the truncated factor to a limited extent, probably via long-range interactions, and induces a destabilisation of the G domain structure. The functional alterations brought about by substitutions on the consensus sequences 18-24 and 80-83 highlight the importance of these residues for the interaction with GTP/GDP and the GTPase activity. Mutations concerning residues interacting with the guanine base lead to proteins in large part insoluble and inactive. In one case, the mutated protein (EF-TuAsn135----Asp) inhibited the growth of the host cell. This demonstrates the crucial role of the base specificity for the active conformation of EF-Tu. The obtained results are discussed in the light of the three-dimensional structure of EF-Tu.
Gümüşel
F
F
Laboratoire de Biochimie, Laboratoire Associè du C.N.R.S., N0240, Ecole Polytechnique, Palaiseau, France.
Cool
R H
RH
Weijland
A
A
Anborgh
P H
PH
Parmeggiani
A
A
D000595:Amino Acid Sequence; D001224:Aspartic Acid; D001665:Binding Sites; D003196:Computer Graphics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D007700:Kinetics; D007930:Leucine; D008239:Lysine; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010444:Peptide Elongation Factor Tu / Q000235:genetics / Q000378:metabolism*; D011487:Protein Conformation; D013816:Thermodynamics; D014508:Urea / Q000494:pharmacology; D014633:Valine
D006153:Guanosine Diphosphate; D001224:Aspartic Acid; D006160:Guanosine Triphosphate; D014508:Urea; D010444:Peptide Elongation Factor Tu; D007930:Leucine; D014633:Valine; D008239:Lysine