Comparative study on cytochrome P-450 of yeasts using specific antibodies to cytochromes P-450alk and P-450(14DM).
The occurrence of cytochrome P-450(14DM) (lanosterol 14 alpha-demethylase) and cytochrome P-450alk (long-chain alkane terminal hydroxylase) in various yeast strains was determined with immunological procedures. Cytochrome P-450(14DM), which is constitutive or housekeeping enzyme playing an essential role in ergosterol biogenesis, was found in all yeast strains so far tested. Cytochromes P-450(14DM) from different species of yeast were immunologically different, although they may have had a few common antigenic sites. In contrast, cytochrome P-450alk was detected only in the alkane-assimilating yeasts.
Kaergel
E
E
Central Institute for Molecular Biology, National Academy of Science of G.D.R., Berlin-Buch.
Aoyama
Y
Y
Schunck
W H
WH
Mueller
H G
HG
Yoshida
Y
Y
D000473:Alkanes / Q000378:metabolism; D000908:Antibodies, Fungal / Q000276:immunology; D000918:Antibody Specificity; D002175:Candida / Q000201:enzymology*; D003429:Cross Reactions; D042926:Cytochrome P-450 CYP4A; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis* / Q000276:immunology; D005779:Immunodiffusion; D008861:Microsomes / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000032:analysis / Q000276:immunology; D010088:Oxidoreductases / Q000032:analysis / Q000276:immunology; D011863:Radioimmunoassay; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D058886:Sterol 14-Demethylase; D015003:Yeasts / Q000201:enzymology*
D000473:Alkanes; D000908:Antibodies, Fungal; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases; D058886:Sterol 14-Demethylase; D042926:Cytochrome P-450 CYP4A