Molecular cloning of DNA from specific chromosomal regions by microdissection and sequence-independent amplification of DNA.
A method that permits the in vitro amplification and cloning of DNA dissected from specific regions of a chromosome and does not require prior knowledge of the DNA sequence is described. DNA from several different chromosomal loci in the Drosophila melanogaster genome has been isolated by this method. Although the procedure was developed to permit the isolation of DNA sequences from serial sections of a single microdissected polytene chromosome, it should be useful for obtaining DNA clones from specific regions of the nonpolytene chromosomes of other organisms as well.
Johnson
D H
DH
Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Florida 33136.
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002875:Chromosomes / Q000648:ultrastructure*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004331:Drosophila melanogaster; D005784:Gene Amplification; D008866:Microsurgery
D004247:DNA