Failure of 1,25-(OH)2-vitamin D3 to affect calcium flux across sheep rumen wall mucosa in vitro.
Isolated pieces of mucosa from the sheep rumen wall mounted between half-chambers were used in conventional incubation studies (1 h, 39 degrees C) with buffer solutions resembling rumen fluid (= mucosal fluid) and extracellular fluid (= serosal fluid), respectively. The mucosal-to-serosal (ms) flux of calcium was studied by use of 45CaCl2 with and without 1,25-(OH)2-vitamin D3 in the serosal incubation fluid. Different concentrations of the vitamin had no effect on Ca ms-flux.
Bödeker
D
D
Department of Physiology, School of Veterinary Medicine, Hannover, FRG.
Mahler
M
M
Höller
H
H
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002117:Calcitriol / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism*; D012417:Rumen / Q000378:metabolism*; D012756:Sheep / Q000378:metabolism*
D002117:Calcitriol; D002118:Calcium