Isolation and characterization of several plasma apolipoproteins of common marmoset monkey.
To explore the potential of the common marmoset monkey (Callithrix jacchus) as a model for human plasma lipoprotein metabolism, several marmoset apolipoproteins were isolated and characterized in this study. Based on several properties, including molecular weight, amino acid composition, and sequence, the marmoset apolipoproteins are strikingly similar to human apolipoprotein (apo) A-I, A-II, C-III, and A-IV. The first 54 residues of marmoset apo A-I showed 87% sequence identity with the corresponding region of human apo A-I. Amino-terminal sequence analysis of a minor basic apo A-I isoform revealed that it contained an amino-terminal hexapeptide extension (Arg-His-Phe-Gln-Gln-) identical to that found in human proapo A-I. Like apo A-II in most nonhuman primates, marmoset apo-A-II differed from human apo A-II in that it did not contain cysteine and therefore existed as a monomer. The complete amino acid sequence of marmoset apo A-II was deduced. The protein contains 77 amino acids, as does human apo A-II, and showed an 82% identity with its human equivalent. In both species, apo C-III and E had similar amino-terminal sequences and amino acid compositions. Like human apo E, marmoset apo E contained minor sialylated isoforms. However, unlike human apo C-III, no sialyated isoforms of marmoset apo C-III were observed. In addition, the marmoset possessed an apolipoprotein whose molecular weight and amino acid composition were similar to those of human apo A-IV. The close structural similarities between corresponding marmoset and human apolipoproteins indicate that the marmoset monkey will be useful as a model for human lipoprotein metabolism.
Crook
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Gladstone Foundation Laboratories for Cardiovascular Disease, Department of Pathology, University of California, San Francisco 94140.
Weisgraber
K H
KH
Rall
S C
SC
Mahley
R W
RW
D000595:Amino Acid Sequence; D000818:Animals; D001053:Apolipoproteins / Q000097:blood / Q000302:isolation & purification* / Q000378:metabolism; D001054:Apolipoproteins A / Q000097:blood / Q000302:isolation & purification; D001057:Apolipoproteins E / Q000097:blood / Q000302:isolation & purification; D002144:Callithrix / Q000097:blood* / Q000378:metabolism; D002143:Callitrichinae / Q000097:blood*; D008954:Models, Biological; D008969:Molecular Sequence Data
D001053:Apolipoproteins; D001054:Apolipoproteins A; D001057:Apolipoproteins E