title: Non-enzymatic covalent binding of radioactivity from [14C]thiourea to rat lung protein.
abstract: In vitro covalent binding of radioactivity from [14C]thiourea (TU) to rat lung protein occurs in the presence of 100% CO2. Prior boiling of lung slices results in a subsequent increase in covalent binding. Covalently bound radioactivity was released from rat lung homogenates and acid-insoluble protein obtained from animals treated with [14C]TU. The release was achieved with ammonium ion. One possibility suggested by these results is that the bound species may involve binding of isothiocyanic acid via a thiocarbamylation reaction.
authors.affiliation: Department of Pharmacology, School of Medicine, University of California, Davis 95616.
authors.forename: M A
authors.initials: MA
authors.lastname: Hollinger
authors.forename: S N
authors.initials: SN
authors.lastname: Giri
mesh_terms: D000085:Acetates / Q000494:pharmacology; D000332:Aerobiosis; D000818:Animals; D002245:Carbon Dioxide / Q000494:pharmacology; D002250:Carbon Radioisotopes; D003486:Cyanides / Q000494:pharmacology; D003545:Cysteine / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D008168:Lung / Q000378:metabolism*; D008297:Male; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013447:Sulfites / Q000494:pharmacology; D013890:Thiourea / Q000378:metabolism*
chemical_list: D000085:Acetates; D002250:Carbon Radioisotopes; D003486:Cyanides; D011506:Proteins; D013447:Sulfites; D002245:Carbon Dioxide; D000431:Ethanol; D013890:Thiourea; D003545:Cysteine; C018824:ammonium acetate