[The demonstration of proliferating cells by using monoclonal antibodies to 5-bromo-2'-deoxyuridine in the peroxidase-antiperoxidase method].
Monoclonal antibodies (MAb) to the thymidine analog 5-bromo-2'-deoxyuridine (BrdUrd) and to horseradish peroxidase (HRPO) have been produced and characterized. On the basis of Mab to HRPO, complexes of the antibodies and HRPO (PAP-complexes) for immunochemical investigations are prepared. The possibility to identify proliferating cells in cultures of mouse myeloma Sp2/0 and mouse fibroblasts NIH 3T3 using Mab to BrdURd by the PAP-method is shown. The conditions of performing the analysis were optimized. The effect of various techniques ot cell fixation and cell DNA denaturation on cell morphology and on specific staining of nuclei of BrdUrd-containing cells in investigated.
Morenkov
O S
OS
Mantsygin
Iu A
IuA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000918:Antibody Specificity / Q000276:immunology; D001973:Bromodeoxyuridine / Q000276:immunology*; D002455:Cell Division / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured / Q000166:cytology / Q000276:immunology; D003584:Cytological Techniques; D005347:Fibroblasts / Q000166:cytology* / Q000276:immunology; D006735:Horseradish Peroxidase / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D007114:Immunization / Q000379:methods; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000276:immunology / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000276:immunology
D000911:Antibodies, Monoclonal; D006735:Horseradish Peroxidase; D001973:Bromodeoxyuridine
Vyiavlenie proliferiruiushchikh kletok s pomoshch'iu monoklonal'nykh antital k 5-brom-2'-dezoksiuridinu metodom peroksidaza--antiperoksidaza.