Production of interferon-gamma by in vivo tumor-sensitized T cells: association with active antitumor immunity.
The state of active immunity to Meth A fibrosarcoma in mice immunized with an admixture of Meth A cells and Propionibacterium acnes is associated with possession by the host of spleen cells capable of producing interferon-gamma (IFN-gamma) upon in vitro restimulation with irradiated tumor cells. The ability of spleen cells from immunized mice to produce IFN-gamma in response to irradiated Meth A cells decays as active antitumor immunity is replaced by a state of immunological memory. The IFN-producing cells are L3T4+Ly2+, cyclophosphamide-sensitive and radiosensitive T cells, as determined by their sensitivity to corresponding monoclonal antibodies and complement. The induction of IFN-gamma production by in vivo tumor-sensitized T cells is tumor specific, in that spleen cells from mice immunized against Meth A fibrosarcoma can produce IFN in response to irradiated Meth A cells but not in response to another syngeneic tumor M109 lung carcinoma.
Bursuker
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Pharmaceutical Research and Development Division, Bristol-Myers Company, Wallingford, CT 06492.
Pearce
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MT
D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology; D005354:Fibrosarcoma / Q000276:immunology*; D005720:Gamma Rays; D007110:Immunity, Active / Q000502:physiology; D007116:Immunization, Passive; D007369:Interferon Inducers / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis*; D007958:Leukocyte Count; D051379:Mice; D009500:Neutralization Tests; D011425:Propionibacterium acnes / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D013934:Thymectomy
D007369:Interferon Inducers; D007371:Interferon-gamma; D003520:Cyclophosphamide