A radioactive assay for the physiological activity of the tryptophan synthetase alpha subunit in crude extracts of Escherichia coli.
A modified assay has been devised for the physiological reaction, indole-3-glycerol phosphate to Trp, of the enzyme tryptophan synthetase. The assay may be applied to crude bacterial extracts, and is based on the measurement of incorporation of radioactivity from [3H]Ser into Trp. Comparison with previous colorimetric assays indicates an improvement in sensitivity of about 30-fold, and advantages in terms of sample economy and simplified manipulation.
Sörensen
P M
PM
Institut de Biologie Physico-Chimique, URA 1139 CNRS, Paris, France.
Hijazi
K A
KA
Murgola
E J
EJ
Buckingham
R H
RH
D003124:Colorimetry; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005069:Evaluation Studies as Topic; D009154:Mutation; D011487:Protein Conformation; D014316:Tritium; D014367:Tryptophan Synthase / Q000032:analysis* / Q000737:chemistry / Q000235:genetics
D014316:Tritium; D014367:Tryptophan Synthase