title: The effect of GDP on rod outer segment G-protein interactions.
abstract: The role of GDP has heretofore been little studied in the analysis of visual receptor G-protein (G) interactions. Here we use kinetically resolved absorption and light scattering spectroscopy, centrifugation, porous membrane filtration, and enzyme assay to compare the effectiveness of GDP with that of GTP or gamma-thio-guanosine-5'-triphosphate in the modulation of G-protein binding to rod disc membranes and activated receptor (R*). We also compare effectiveness of GDP with that of GTP in the separation of G alpha and G beta gamma subunits and in activation of effector, cGMP phosphodiesterase. We find that when different nucleotide affinities are taken into account, actions such as the release of G from R* binding, earlier ascribed to GTP alone, are also typical of GDP. The principal specific actions of GTP that occur only weakly or undetectably for GDP are, respectively, the release of G-protein subunits from the membrane into solution and activation of phosphodiesterase. While GDP, like GTP, releases G-protein binding to receptor, we argue that GDP cannot mediate G-protein subunit separation, even on the membrane surface. GDP retained on G-protein after GTP hydrolysis may function to prevent tight binding to quiescent receptors in a manner analogous to its action on G-protein binding to activated receptors. Weak binding of G.GDP may function to accelerate receptor catalyzed amplification during transduction.
authors.affiliation: Department of Anatomy, University of Pennsylvania School of Medicine, Philadelphia 19104-6058.
authors.forename: J
authors.initials: J
authors.lastname: Panico
authors.forename: J H
authors.initials: JH
authors.lastname: Parkes
authors.forename: P A
authors.initials: PA
authors.lastname: Liebman
mesh_terms: D000818:Animals; D002417:Cattle; D003624:Darkness; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006153:Guanosine Diphosphate / Q000494:pharmacology*; D006160:Guanosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D008027:Light; D008433:Mathematics; D008962:Models, Theoretical; D012374:Rod Cell Outer Segment / Q000378:metabolism*; D012542:Scattering, Radiation; D013053:Spectrophotometry
chemical_list: D006153:Guanosine Diphosphate; D006160:Guanosine Triphosphate; D019204:GTP-Binding Proteins