Comparison of beta-galactosidase production by two inducible promoters in Myxococcus xanthus.
The inducibility of two promoter systems, one heterologous and one homologous, has been assessed in the Gram-negative bacterium Myxococcus xanthus. The heterologous system involved the hybrid tac promoter and the presence of lacIq, the lac repressor from Escherichia coli. This system is inducible in its natural host with isopropyl-beta-D-thiogalactopyranoside (IPTG). The homologous promoter system involves the light-inducible carQRS promoter, which is normally involved in the expression of the regulators of the light-inducible light-protective carotenoid synthesis regulon in M. xanthus. In each case, promoter activity and strength was assayed using the E. coli gene lacZ. In our constructs, which were present in a single copy in the M. xanthus chromosome, the carQRS promoter yielded at least a 47-fold increase in beta-galactosidase production upon light induction, whilst IPTG increased by 8-fold the amount of enzyme produced under the control of the ptac-lacIq system. Regulation by the latter was significantly higher than that obtained with the unmodified lacZ promoter.
Letouvet-Pawlak
B
B
Laboratoire de Microbiologie URA 203, CNRS, Faculté des Sciences de Rouen, Mont Saint Aignan, France.
Monnier
C
C
Barray
S
S
Hodgson
D A
DA
Guespin-Michel
J F
JF
D004790:Enzyme Induction / Q000187:drug effects; D005696:Galactosidases / Q000096:biosynthesis*; D005827:Genetics, Microbial; D066298:In Vitro Techniques; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology*; D009231:Myxococcales / Q000187:drug effects / Q000201:enzymology*; D010775:Photic Stimulation; D011401:Promoter Regions, Genetic / Q000187:drug effects; D013865:Thioglycosides / Q000494:pharmacology*; D001616:beta-Galactosidase / Q000096:biosynthesis*
D013865:Thioglycosides; D007544:Isopropyl Thiogalactoside; D005696:Galactosidases; D001616:beta-Galactosidase