Detection of N-acetylcysteine, cysteine and their disulfides in urine by liquid chromatography with a dual-electrode amperometric detector.
A method for the determination of N-acetylcysteine, cysteine and their disulfides in urine is described. The thiols and disulfides are separated by reversed-phase ion-pair chromatography with octyl sodium sulfate as the ion-pairing reagent and detected with a dual-electrode amperometric detector using Au/Hg amalgam electrodes. Both the thiols and disulfides are detected with this system. In addition, dimers and mixed disulfides can be detected individually.
Stenken
J A
JA
Department of Chemistry, University of Kansas, Lawrence 66045.
Puckett
D L
DL
Lunte
S M
SM
Lunte
C E
CE
D000111:Acetylcysteine / Q000652:urine*; D002853:Chromatography, Liquid; D003545:Cysteine / Q000652:urine*; D004220:Disulfides / Q000652:urine*; D004563:Electrochemistry; D006801:Humans
D004220:Disulfides; D003545:Cysteine; D000111:Acetylcysteine