Portal-drained visceral flux of nutrients in lambs fed alfalfa or maintained by total intragastric infusion.
An experiment was performed using lambs fitted with chronic indwelling catheters in appropriate blood vessels for portal-drained visceral (PDV) flux measurements. The objective of the experiment was to evaluate PDV nutrient flux in alfalfa-fed and intragastrically infused lambs and to evaluate the effects of amount of energy and N infused on PDV nutrient metabolism. Lambs were fed alfalfa or infused with 1.64 and 10.9; 1.82 and 12.3; or 2.37 and 15.0 Mcal GE and g N/d, respectively. Arterial concentrations and PDV fluxes of glucose, L-lactate, acetate and portal blood flow were not different (P greater than .10) between alfalfa-fed and infused lambs. Net flux of alpha-amino N, ammonia N and branched-chain VFA were lower (P less than .05) and net flux of propionate, butyrate and total VFA were higher for intragastric infusion vs alfalfa. No consistent differences in PDV fluxes were noted among the three levels of energy and N infused, although the energy and N levels tested were near maintenance requirements. Nitrogen retention increased as level of energy and N infusion increased. Approximately 47, 70 and 22% of ruminally infused acetate, propionate and butyrate, respectively, were found on a net basis in portal blood as VFA. Measurements of net nutrient utilization by the PDV that eliminate the influence of ruminal fermentation are possible. How the changes in PDV tissues due to intragastric infusion influence these estimates is unknown.
Gross
K L
KL
Kansas State University, Manhattan 66506.
Harmon
D L
DL
Avery
T B
TB
D000821:Animal Feed; D000818:Animals; D002408:Catheters, Indwelling / Q000662:veterinary; D004750:Enteral Nutrition / Q000662:veterinary*; D005232:Fatty Acids, Volatile / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008297:Male; D000455:Medicago sativa; D009584:Nitrogen / Q000378:metabolism; D011168:Portal System / Q000378:metabolism; D012417:Rumen / Q000032:analysis; D012756:Sheep / Q000378:metabolism*; D014781:Viscera / Q000378:metabolism*
D005232:Fatty Acids, Volatile; D009584:Nitrogen