Transmembrane potential measurement with carbocyanine dye diS-C3-(5): fast fluorescence decay studies.
The mechanism of carbocyanine dye diS-C3-(5) fluorescence intensity variations with transmembrane potential changes has been studied using time-resolved fluorescence spectroscopy. Clear evidence is given of the transmembrane-potential-dependent partition of the dye among various sites with different fluorescence lifetimes. It was found that fluorescence decay profiles reflect the transmembrane potential changes.
Síp
M
M
Institute of Physics, Charles University, Prague, Czechoslovakia.
Herman
P
P
Plásek
J
J
Hrouda
V
V
D052160:Benzothiazoles; D002138:Calibration; D002232:Carbocyanines; D004058:Diffusion; D005456:Fluorescent Dyes; D006801:Humans; D008564:Membrane Potentials; D011188:Potassium / Q000502:physiology; D011804:Quinolines; D013050:Spectrometry, Fluorescence; D013997:Time Factors; D014407:Tumor Cells, Cultured
D052160:Benzothiazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; D011804:Quinolines; C012944:3,3'-dipropyl-2,2'-thiadicarbocyanine; D011188:Potassium