Elongation factor EF-1 alpha from Artemia is specifically labeled at Lys-63 by the guanine nucleotide analog pyridoxal-5'-diphospho-5'-guanosine.
We have synthesized the guanine nucleotide analog pyridoxal-5'-diphospho-5'-guanosine. This compound specifically modifies a single lysine residue in elongation factor 1 alpha from Artemia, indicating that this residue is in close contact with the reactive part of the guanine nucleotide analog. This result is discussed in terms of the structure of the nucleotide-binding domain of the factor.
Amons
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Department of Medical Biochemistry, Sylvius Laboratory, State University of Leiden, The Netherlands.
Schipper
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A
Möller
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W
D000345:Affinity Labels; D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia / Q000032:analysis*; D001665:Binding Sites; D001667:Binding, Competitive; D006150:Guanine Nucleotides; D006153:Guanosine Diphosphate / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D008239:Lysine; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000378:metabolism*
D000345:Affinity Labels; D006150:Guanine Nucleotides; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; C057277:guanosine diphosphopyridoxal; D006153:Guanosine Diphosphate; D008239:Lysine