title: Occurrence in the archaebacterium Sulfolobus solfataricus of a ribosomal protein complex corresponding to Escherichia coli (L7/L12)4.L10 and eukaryotic (P1)2/(P2)2.P0.
abstract: Two-dimensional electrophoresis of total protein from 50 S ribosomal subunits of the archaebacterium Sulfolobus solfataricus demonstrated a complex between two proteins that was stable in 6 M urea, but dissociable in detergent or below pH 5.5. The proteins, numbered L1 and L10 according to their electrophoretic mobilities, corresponded to Escherichia coli ribosomal proteins L10 and L7/L12, respectively. The members of the complex were therefore designated Sso L10e and Sso L12e. Sso L12e had other properties in common with E. coli L7/L12: low molecular weight, relative acidity, selective release from the ribosome by high salt/ethanol, and dimeric structure. The Sso L12e.Sso L10e complex was isolated by gel filtration of total 50 S proteins in 4 M urea. The stoichiometry of the components was approximately four copies of Sso L12e to one copy of Sso L10e. The occurrence in an archaebacterium of a complex of acidic ribosomal proteins similar to E. coli (L7/L12)4.L10 and eukaryotic (P1)2/(P2)/.P0 strongly supports the concept that this element of quaternary structure is a major conserved feature of the ribosome and reaffirms its importance in the translocation step of protein synthesis.
authors.affiliation: Department of Biological Chemistry, School of Medicine, University of California, Davis 95616.
authors.forename: C
authors.initials: C
authors.lastname: Casiano
authors.forename: A T
authors.initials: AT
authors.lastname: Matheson
authors.forename: R R
authors.initials: RR
authors.lastname: Traut
mesh_terms: D001105:Archaea / Q000032:analysis*; D002850:Chromatography, Gel; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000032:analysis*; D008970:Molecular Weight; D010750:Phosphoproteins; D000080882:Ribosomal Protein L10; D012269:Ribosomal Proteins / Q000302:isolation & purification*; D009626:Terminology as Topic
chemical_list: D010750:Phosphoproteins; D012269:Ribosomal Proteins; C031937:phosphoprotein P2, ribosomal; C035690:ribosomal phosphoprotein P1; C054675:ribosomal protein P0; C016700:ribosomal protein L7-L12