title: Development of a beta-galactosidase alpha-complementation system for molecular cloning in Bacillus subtilis.
abstract: A versatile beta-galactosidase alpha-complementation system for Bacillus subtilis was developed, which can be used for molecular cloning in this Gram+ organism. The cloning system, which is based on the highly efficient host-vector system 6GM-pHP13, offers several advantages over previously described systems: (1) convenient direct selection of recombinants; (2) the cloning of large heterologous DNA fragments with high efficiency; and (3) the availability of six unique target sites: SphI, NdeI, NheI, BamHI, SmaI and EcoRI.
authors.affiliation: Department of Microbiology, Center of Biological Sciences, Haren, The Netherlands.
authors.forename: P
authors.initials: P
authors.lastname: Haima
authors.forename: D
authors.initials: D
authors.lastname: van Sinderen
authors.forename: H
authors.initials: H
authors.lastname: Schotting
authors.forename: S
authors.initials: S
authors.lastname: Bron
authors.forename: G
authors.initials: G
authors.lastname: Venema
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D005696:Galactosidases / Q000235:genetics*; D015870:Gene Expression; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D001616:beta-Galactosidase / Q000235:genetics*
chemical_list: D005696:Galactosidases; D001616:beta-Galactosidase