Bacillus amyloliquefaciens alpha-amylase signal sequence fused in frame with human proinsulin is properly processed by Bacillus subtilis cells.
The plasmid pBINS1, containing the promoter, SD and leader peptide sequences of Bacillus amyloliquefaciens alpha-amylase gene and 267 bp long sequence coding for human proinsulin directs the efficient synthesis of hybrid preproinsulin, as well as quantitative secretion of proinsulin outside of protease-deficient Bacillus subtilis AJ73 cells. The recombinant proinsulin has been isolated from the culture medium and its N-terminal sequence shown to be identical with that of natural human prohormone.
Novikov
A A
AA
Institute of Molecular Genetics, USSR Academy of Sciences, Moscow.
Borukhov
S I
SI
Strongin AYa
D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D001412:Bacillus subtilis / Q000235:genetics*; D003001:Cloning, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D011384:Proinsulin / Q000096:biosynthesis / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D000516:alpha-Amylases / Q000235:genetics*
D011994:Recombinant Proteins; D011384:Proinsulin; D000516:alpha-Amylases