title: Chemical synthesis of oligoribonucleotides for structural studies.
abstract: Large-scale synthesis of oligoribonucleotides has been performed successfully on a solid support by the phosphoramidite approach using levulinyl and tetrahydrofuranyl protection for the 5'- and 2'-hydroxyl groups respectively. A hexamer containing inosine and four fragments of a hammerhead-type ribozyme have been synthesized on a 10 mumol scale for structural studies by NMR spectroscopy and X-ray crystallography.
authors.affiliation: Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
authors.forename: S
authors.initials: S
authors.lastname: Iwai
authors.forename: S
authors.initials: S
authors.lastname: Tsuda
authors.forename: K
authors.initials: K
authors.lastname: Hikichi
authors.forename: E
authors.initials: E
authors.lastname: Ohtsuka
mesh_terms: D001483:Base Sequence; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D016337:RNA, Catalytic / Q000737:chemistry
chemical_list: D009843:Oligoribonucleotides; D016337:RNA, Catalytic