[Production and characteristics of monoclonal antibodies reacting with human-type M. tuberculosis].
Monoclonal antibodies to M. tuberculosis were produced by hybrid technology. They were described via enzyme immunoassay and immunoblotting. It was shown that these antibodies should be included into IgG class. Besides, they are oriented towards and antigen with a molecular weight of 20 kDa, react with H37Rv at a concentration of 12 ng/ml and with BCG at a concentration of 50 micrograms/ml and fail to react with M. Intracellulare, M. Scrofulaceum and E. coli.
Chernousova
L N
LN
Kulikovskaia
N V
NV
Razumovskaia
V S
VS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D015269:Mycobacterium avium Complex / Q000276:immunology; D009163:Mycobacterium bovis / Q000276:immunology; D015359:Mycobacterium scrofulaceum / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G
Poluchenie i kharakteristika monoklonal'nykh antitel, reagiruiushchikh s M. tuberculosis vida humanus.