Regulation of poly(ADP-ribose) polymerase mRNA levels during compensatory and mitogen-induced growth of rat liver.
Poly(ADP-ribose) polymerase mRNA levels were studied in hepatic regeneration following partial hepatectomy, and in hyperplasia induced by the mitogen lead nitrate. A significant increase in the level of poly(ADP-ribose) polymerase mRNA was found 8 h after partial hepatectomy when no detectable increase of DNA synthesis could be observed; the level of poly(ADP-ribose) polymerase transcripts increased up to six-fold within 1-2 days. A similar increase of the level of poly(ADP-ribose) polymerase mRNA was found 24 h after treatment with lead nitrate. A twofold increase in poly(ADP-ribose) polymerase activity was observed 2 days after (a) partial hepatectomy and (b) lead nitrate treatment. From these results an important role of poly(ADP-ribose) polymerase in cell proliferation could be suggested.
Menegazzi
M
M
Istituto di Chimica Biologica, Università di Verona, Italy.
De Prati
A C
AC
Ledda-Columbano
G M
GM
Columbano
A
A
Uchida
K
K
Miwa
M
M
Suzuki
H
H
D000818:Animals; D002455:Cell Division; D002467:Cell Nucleus / Q000201:enzymology; D004247:DNA / Q000096:biosynthesis; D015870:Gene Expression / Q000187:drug effects; D007854:Lead / Q000494:pharmacology; D008099:Liver / Q000201:enzymology / Q000254:growth & development / Q000502:physiology*; D008115:Liver Regeneration; D009566:Nitrates / Q000494:pharmacology; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D009566:Nitrates; D012333:RNA, Messenger; D007854:Lead; C017461:lead nitrate; D004247:DNA; D011065:Poly(ADP-ribose) Polymerases