title: Pseudomonas putida KF715 bphABCD operon encoding biphenyl and polychlorinated biphenyl degradation: cloning, analysis, and expression in soil bacteria.
abstract: We cloned the entire bphABCD genes encoding degradation of biphenyl and polychlorinated biphenyls to benzoate and chlorobenzoates from the chromosomal DNA of Pseudomonas putida KF715. The nucleotide sequence revealed two open reading frames corresponding to the bphC gene encoding 2,3-dihydroxybiphenyl dioxygenase and the bphD gene encoding 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (ring-meta-cleavage compound) hydrolase.
authors.affiliation: Fermentation Research Institute, Agency of Industrial Science and Technology, Ibaraki, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Hayase
authors.forename: K
authors.initials: K
authors.lastname: Taira
authors.forename: K
authors.initials: K
authors.lastname: Furukawa
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001565:Benzoates / Q000378:metabolism; D001713:Biphenyl Compounds / Q000378:metabolism*; D003001:Cloning, Molecular; D049308:Dioxygenases; D015870:Gene Expression; D005798:Genes, Bacterial; D006867:Hydrolases / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon; D010105:Oxygenases / Q000235:genetics*; D011078:Polychlorinated Biphenyls / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics*; D015183:Restriction Mapping; D012988:Soil Microbiology
chemical_list: D001565:Benzoates; D001713:Biphenyl Compounds; D011078:Polychlorinated Biphenyls; D010105:Oxygenases; D049308:Dioxygenases; C074272:2,3-dihydroxybiphenyl oxygenase; D006867:Hydrolases; C062907:2,6-dioxo-6-phenylhexa-3-enoate hydrolase