title: Performance evaluation of an aqueous-organic phase separator for post-column reactions in high-performance liquid chromatography, and its application to the enhanced detection of some basic drugs of abuse.
abstract: A phase separator is described that is suitable for post-column HPLC applications. It operates with commonly used HPLC eluents and immiscible organic solvents as long as the two phases remain immiscible. It is compatible with gradient elution systems. Separation efficiency is routinely better than 0.8, which ensures that analyte peak heights are about 95% of the maximum height under these conditions. An application for the detection of pethidine, cocaine, methadone, piritramide and dipipanone at 0.8-1.8 ng on-column loadings is described.
authors.affiliation: School of Pharmacy and Pharmacology, University of Bath, Avon, UK.
authors.forename: I M
authors.initials: IM
authors.lastname: Roy
authors.forename: T M
authors.initials: TM
authors.lastname: Jefferies
mesh_terms: D002851:Chromatography, High Pressure Liquid; D013287:Illicit Drugs / Q000032:analysis*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
chemical_list: D013287:Illicit Drugs; D012997:Solvents