title: Isolation of a Y chromosomal DNA sequence and its clinical application.
abstract: A 4.6 kb long, Y-specific DNA fragment was isolated from a flow-sorted human Y chromosomal library, and its male specificity was confirmed by Southern blot analysis. The fragment, designated as pY-80, was proven with an in situ hybridization experiment to have originated from the Yp11.2-Ypter region. Its 2,808 bp section was sequenced. The polymerase chain reaction proceeded with oligonucleotides flanking a 666 bp PstI-EcoRI fragment of the sequence as primers and a male genomic DNA as a template, but not with a female genomic DNA. Preliminary tests of samples of various sources successfully detected the Y-specific fragment in male-derived samples, including mouth wash, single hair roots, urinary epithelial cells, dried blood spots and amniotic fluid cells.
authors.affiliation: Department of Pediatrics, Yamaguchi University School of Medicine, Ube, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tsukahara
authors.forename: S
authors.initials: S
authors.lastname: Matsuura
authors.forename: F
authors.initials: F
authors.lastname: Kishi
authors.forename: A
authors.initials: A
authors.lastname: Yoshida
authors.forename: T
authors.initials: T
authors.lastname: Kajii
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014998:Y Chromosome / Q000737:chemistry*
chemical_list: D004247:DNA