Rapid and efficient detection of genetic polymorphism in wheat through amplification by polymerase chain reaction.
The polymerase chain reaction (PCR) was used to amplify genomic DNA of several wheat genotypes. The oligonucleotides used as primers were the terminal sequences of a gamma-gliadin gene. The electrophoretic analysis of the PCR products showed specific bands which revealed both inter- and intra-specific genetic polymorphism among the examined genotypes. The technique is proposed as a very simple and efficient alternative to RFLP markers.
D'Ovidio
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Dipartimento di Agrobiologia e Agrochimica, Università della Tuscia, Viterbo, Italy.
Tanzarella
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OA
Porceddu
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D001483:Base Sequence; D004247:DNA / Q000235:genetics; D005784:Gene Amplification; D005819:Genetic Markers; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D014908:Triticum / Q000235:genetics
D005819:Genetic Markers; D004247:DNA