Potassium currents activated in hippocampal neurons by serotonin are mediated by a change in intracellular calcium concentrations.
Intracellular activity was recorded from CA1 neurons in the slice preparation, by using K-acetate-, Bapta- or EGTA-filled micropipettes. The calcium chelator, Bapta, clamps [Ca]i more rapidly than EGTA and is less associated with pH changes. Neurons recorded with Bapta-filled micropipettes were much less sensitive to 5-HT and baclafen than those recorded with the standard K-acetate- or EGTA-filled micropipetes. These results suggest that [Ca]i is important for the generation of the 5-HT-dependent potassium current.
Segal
M
M
Center for Neurosciences, Weizmann Institute of Science, Rehovot, Israel.
D000818:Animals; D001418:Baclofen / Q000494:pharmacology; D002118:Calcium / Q000502:physiology*; D004533:Egtazic Acid / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects; D066298:In Vitro Techniques; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D015221:Potassium Channels / Q000187:drug effects*; D051381:Rats; D012701:Serotonin / Q000502:physiology*
D015221:Potassium Channels; D012701:Serotonin; D004533:Egtazic Acid; D001418:Baclofen; D002118:Calcium