[A specific protein inhibiting membrane permeation of chloramphenicol in Pseudomonas aeruginosa].
A specific protein (MW 18,000) was found using chloramphenicol (CP) base-affinity chromatography of periplasmic-space proteins obtained from an impermeability-type CP-resistant Pseudomonas aeruginosa harboring plasmid kR102. Membrane reconstitution experiments using a liposome system appeared to indicate that the permeability of CP was inhibited by the specific protein.
O'Hara
K
K
Department of Microbiology, Tokyo College of Pharmacy.
Fukuda
H
H
Kanda
T
T
Kono
M
M
D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification*; D002463:Cell Membrane Permeability; D002701:Chloramphenicol / Q000493:pharmacokinetics* / Q000494:pharmacology; D002846:Chromatography, Affinity; D004352:Drug Resistance, Microbial; D008970:Molecular Weight; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000378:metabolism*
D001426:Bacterial Proteins; D002701:Chloramphenicol