The induction of micronucleated polychromatic erythrocytes in mice using single and multiple treatments.
Studies are reviewed in which the effect of treatment/sample protocol on the induction of micronucleated polychromatic erythrocytes (MN-PCE) in male B6C3F1 mice by 3 carcinogens (benzidine, dimethylbenzanthracene and mitomycin C) were evaluated. 3 different treatment/sampling protocols were used, involving from 1 to 3 consecutive daily treatments and from 3 to 1, respectively, consecutive daily samplings beginning 24 h after the last injection. The results indicate that the 3-day injection/single sample time protocol eliminates the need for multiple sample times, minimizes the number of animals required in a study, decreases the time needed for data collection and simplifies data analysis. A comparison of the frequency of induced MN-PCE in peripheral blood and bone marrow suggests that, following a 3-injection protocol, either tissue can be used with equal efficiency.
Tice
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RR
Integrated Laboratory Systems, Research Triangle Park, NC 27709.
Erexson
G L
GL
Shelby
M D
MD
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D001560:Benzidines / Q000008:administration & dosage / Q000494:pharmacology; D002273:Carcinogens / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D004912:Erythrocytes / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects*; D015162:Micronucleus Tests / Q000379:methods; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology
D001560:Benzidines; D002273:Carcinogens; D008937:Mitomycins; C029876:benzidine; D016685:Mitomycin; D015127:9,10-Dimethyl-1,2-benzanthracene