Estimation of D segment usage in initial D to JH joinings in a murine immature B cell line. Preferential usage of DFL16.1, the most 5' D segment and DQ52, the most JH-proximal D segment.
We established SPL2-1-2, a murine immature B cell line transformed by tsOS-59, a temperature-sensitive mutant of Abelson murine leukemia virus. SPL2-1-2 has a VHDJH-/G (germ-line) configuration, and can continuously generate D to JH joinings from the germ-line allele during culture. The D to JH joinings are strongly promoted by the shift of the culture temperature from permissive (35 degrees C) to non-permissive temperature (39 degrees C). Using this H chain gene rearrangement system, we succeeded in the estimation of the frequencies of D segment usage in initial D to JH joinings by deletion-mapping analysis. The results demonstrated the preferential utilization of DFL16.1, the most 5' D segment and DQ52, the most JH-proximal D segment in initial D to JH joinings.
Tsukada
S
S
Third Department of Internal Medicine, Osaka University Medical School, Japan.
Sugiyama
H
H
Oka
Y
Y
Kishimoto
S
S
D000483:Alleles; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000502:physiology*; D015139:Blotting, Southern; D002454:Cell Differentiation; D002872:Chromosome Deletion; D004252:DNA Mutational Analysis; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D005803:Genes, Immunoglobulin; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D066298:In Vitro Techniques; D051379:Mice; D011995:Recombination, Genetic; D013696:Temperature
D007143:Immunoglobulin Heavy Chains