Comparison of the properties of glucoamylases from Rhizopus niveus and Aspergillus niger.
Some properties of the glucoamylase from Rhizopus niveus have been determined and compared with the comparable properties of the glucoamylase from Aspergillus niger. The enzymes from these organisms possess the following common properties: quantitative conversion of starch to glucose, molecular weights in the range 95,500 to 97,500, and glycoprotein structures with many oligosaccharide side chains attached to the protein moieties of the enzymes. Differences in the glucoamylases exist in electrophoretic mobility, amino acid composition, nature of carbohydrate units, and types of glycosidic linkages. Lysine, threonine, serine, glutamic acid, tyrosine, and phenylalanine differ in the two glucoamylases by 25 to 50%. Whereas the enzyme from R. niveus contains mannose and glucosamine, in the N-acetyl form, as the carbohydrate constituents, the enzyme from A. niger contains mannose, glucose, and galactose. The carbohydrate chains of the R. niveus enzyme are linked by O-glycosidic and N-glycosidic linkages to the protein, while those of the A. niger enzyme are linked by O-glycosidic linkages only. Antibodies directed against the two glucosamylases have been isolated by affinity chromatography and found to be specific for the carbohydrate units of the glucoamylases. Cross reactions did not occur between the glucoamylases and the purified antibodies.
Pazur
J H
JH
Paul M. Althouse Laboratory, Pennsylvania State University, University Park 16802.
Liu
B L
BL
Miskiel
F J
FJ
D000596:Amino Acids / Q000032:analysis; D001234:Aspergillus niger / Q000201:enzymology*; D002236:Carbohydrate Conformation; D002241:Carbohydrates / Q000032:analysis; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D008401:Gas Chromatography-Mass Spectrometry; D005087:Glucan 1,4-alpha-Glucosidase / Q000032:analysis / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D008745:Methylation; D008970:Molecular Weight; D012233:Rhizopus / Q000201:enzymology*; D013379:Substrate Specificity
D000596:Amino Acids; D002241:Carbohydrates; D005087:Glucan 1,4-alpha-Glucosidase