Sequences of the genes encoding argininosuccinate synthetase in Escherichia coli and Saccharomyces cerevisiae: comparison with methanogenic archaebacteria and mammals.
The nucleotide (nt) sequences of the genes encoding argininosuccinate synthetase from Escherichia coli K-12 (argG) and Saccharomyces cerevisiae (ARG1) were determined. The deduced amino-acid sequences were compared to each other and to their counterparts in two methanogens and in mammals. Three regions are highly conserved. Two of them appear to contain possible Walker-type nt-binding sites [Walker et al., EMBO J. 1 (1982) 945-951] and are therefore candidates for ATP-binding sites. The third region shows some similarity to a short portion of the N-proximal part of the PurA enzyme which catalyses an analogous reaction.
Van Vliet
F
F
Vrije Universiteit Brussel, Belgium.
Crabeel
M
M
Boyen
A
A
Tricot
C
C
Stalon
V
V
Falmagne
P
P
Nakamura
Y
Y
Baumberg
S
S
Glansdorff
N
N
D000595:Amino Acid Sequence; D000818:Animals; D001105:Archaea / Q000235:genetics; D001124:Argininosuccinate Synthase / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005800:Genes, Fungal; D008322:Mammals / Q000235:genetics; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D001124:Argininosuccinate Synthase