[Detection and antigenic distribution of hepatitis B virus in liver tissue and its relation to other serological markers of viral replication].
The aim of this work has been the production of specific monoclonal antibodies against HBV-antigens and their utilisation in order to study their distribution on liver tissue. The monoclonal antibodies anti-HBc and anti-HBs were obtained by the modified hybridoma technique. This study was performed on 50 patients affected by several chronic hepatopathies. For the detection of the antigens, avidin-biotin-peroxidase complex immunostaining was used. Both cytoplasmic and membranous HBsAg were detected in 15 out of 16 HBsAg+ patients; 8 of 12 HBsAg-/anti-HBc+ patients and 1 HBsAg-/antiHBc- patient. Cytoplasmic and nuclear HBcAg was observed in 12 of 16 HBsAg+ patients and 4 of 20 HBsAg- patients. Although the presence of serum HBsAg is an index of liver infection, in some HBsAg-/antiHB+ patients (20%) with undetectable levels of HBsAg, hepatic injury may be disclosed by the detection of other markers of active viral replication.
Malavé
C
C
Departamento de Microbiología e Inmunología, Facultad de Medicina, Universidad del País Vasco, UPV/EHU, Lioa, Vizcaya.
Fernández de Aránguiz
A
A
Sarria
L
L
Ereño
C
C
Campelo
C
C
Gorriño
M T
MT
Suñén
E
E
Cisterna
R
R
D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D015415:Biomarkers; D002462:Cell Membrane / Q000276:immunology; D002467:Cell Nucleus / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003593:Cytoplasm / Q000276:immunology; D005260:Female; D015658:HIV Infections / Q000150:complications; D006509:Hepatitis B / Q000150:complications / Q000382:microbiology*; D006510:Hepatitis B Antibodies; D006511:Hepatitis B Antigens / Q000032:analysis*; D006515:Hepatitis B virus / Q000276:immunology / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D008099:Liver / Q000382:microbiology* / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D006510:Hepatitis B Antibodies; D006511:Hepatitis B Antigens
Detección y distribución antigénica del virus de la hepatitis B en tejido hepático y su relación con otros marcadores serológicos de replicación viral.