Enzymatic and immunological properties of alkaline phosphatase of bullfrog.
Enzymatic and immunological properties of alkaline phosphatase (ALPase) in several tissues of bullfrog (Rana catesbeiana) were investigated. Inhibition and thermal inactivation studies showed that bullfrog ALPases in kidney, liver, and intestine had similar enzymatic properties. In addition, mouse antiserum against bullfrog liver ALPase cross-reacted with kidney and intestine enzymes as well as with liver enzyme. These results suggest that a single phenotype of ALPase exists in all tissues of bullfrog in contrast to two or three isoenzymes in mammals.
Goseki
M
M
Department of Biochemistry, School of Dentistry, Tokyo Medical and Dental University, Japan.
Oida
S
S
Sasaki
S
S
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D002347:Carps; D003370:Coturnix; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D006801:Humans; D005779:Immunodiffusion; D007422:Intestines / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D051379:Mice; D012911:Snakes; D013552:Swine; D014714:Vertebrates / Q000378:metabolism; D014982:Xenopus laevis / Q000378:metabolism*
D000469:Alkaline Phosphatase