Sensitive detection of Mycoplasma pulmonis by using the polymerase chain reaction.
Detection of Mycoplasma pulmonis was examined by using the polymerase chain reaction (PCR) for amplifying a specific DNA sequence. In gel electrophoresis which was conducted to detect the amplified products, only 1 pg of M. pulmonis DNA could be detected following 30 cycles of amplification, while no amplified product was detected even from 1 microgram of M. arthritidis or M. neurolyticum DNA. Furthermore, 10 colony-forming units of M. pulmonis could be detected by direct amplification from the mycoplasma suspension. These results suggest the usefulness of the PCR as a highly sensitive, specific, and rapid method for direct detection of M. pulmonis.
Kunita
S
S
Department of Laboratory Animal Science, School of Hygienic Sciences, Kitasato University, Sagamihara-shi, Japan.
Terada
E
E
Goto
K
K
Kagiyama
N
N
D001483:Base Sequence; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004259:DNA-Directed DNA Polymerase; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D009177:Mycoplasma pneumoniae / Q000302:isolation & purification*; D009841:Oligonucleotides; D016133:Polymerase Chain Reaction
D004269:DNA, Bacterial; D009841:Oligonucleotides; D004259:DNA-Directed DNA Polymerase