Regulatory role of arachidonic acid-derived metabolites for proliferation of transformed murine Leydig cell in serum-free culture condition.
The growth-regulatory ability of eicosanoids from arachidonic acid is poorly understood. To investigate their role in cell growth, we cultured transformed murine Leydig cells (B-1 or B-1 F) in serum-free medium supplemented with various compounds modulating arachidonic acid metabolism. The addition of 5-lipoxygenase inhibitors (AA 861, NDGA and quercetin) showed a growth-stimulative effect. On the other hand, their growth was remarkably inhibited by arachidonic acid added into the culture medium. This growth-inhibiting ability of arachidonic acid was almost completely reversed by a simultaneous exposure of B-1 cells to lipoxygenase inhibitor. Exogenously added 5-HETE inhibited cell proliferation in a dose-dependent manner. These results suggest that 5-lipoxygenase-catalyzed products from arachidonic acid have the ability to suppress the proliferation of some transformed cells.
Nishizawa
Y
Y
Third Department of Internal Medicine, Osaka University Hospital, Japan.
Nishii
K
K
Kishimoto
S
S
Matsumoto
K
K
Sato
B
B
D000098:Acetophenones / Q000494:pharmacology; D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism*; D001091:Arachidonate Lipoxygenases / Q000378:metabolism*; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism* / Q000494:pharmacology; D002455:Cell Division; D002460:Cell Line; D007213:Indomethacin / Q000494:pharmacology; D015289:Leukotrienes / Q000494:pharmacology; D007984:Leydig Cell Tumor / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D016859:Lipoxygenase Inhibitors; D051379:Mice
D000098:Acetophenones; D001095:Arachidonic Acids; D015289:Leukotrienes; D016859:Lipoxygenase Inhibitors; D016718:Arachidonic Acid; C025348:arachidonic acid 5-hydroperoxide; D001091:Arachidonate Lipoxygenases; D001094:Arachidonate 5-Lipoxygenase; C006821:4-bromophenacyl bromide; D007213:Indomethacin