title: Isolation of an enzyme catalysing nitrosamine formation in Pseudomonas aeruginosa and Neisseria mucosae.
abstract: An enzyme catalysing nitrosamine formation was isolated and purified from two denitrifying microorganisms, Pseudomonas aeruginosa and Neisseria mucosae. The soluble enzyme has a molecular weight of 66 as determined by gel filtration and SDS-polyacrylamide gel electrophoresis and a pH optimum for P. aeruginosa of 7.25. A number of microorganisms isolated from human infections have previously been found to possess nitrosating enzymes.
authors.affiliation: International Agency for Research on Cancer, Lyon, France.
authors.forename: S
authors.initials: S
authors.lastname: Calmels
authors.forename: N
authors.initials: N
authors.lastname: Dalla Venezia
authors.forename: H
authors.initials: H
authors.lastname: Bartsch
mesh_terms: D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008970:Molecular Weight; D009343:Neisseria / Q000201:enzymology*; D009602:Nitrosamines / Q000378:metabolism*; D010088:Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000201:enzymology*
chemical_list: D009602:Nitrosamines; D010088:Oxidoreductases; C065724:nitrosating enzyme