Outer membrane protein D2 catalyzes facilitated diffusion of carbapenems and penems through the outer membrane of Pseudomonas aeruginosa.
The outer membrane of imipenem-resistant mutants of Pseudomonas aeruginosa with decreased permeability to imipenem was shown by Western (immuno-) blotting to contain protein D1 and to lack protein D2. Protein D2 was purified and was shown to allow the permeation of imipenem at a rate higher than expected from its molecular weight. Spontaneous imipenem-resistant mutants of P. aeruginosa PAO1 appeared at a frequency of 10(-8) in the laboratory and did not synthesize protein D2. Experiments performed with intact cells carrying plasmid pHN4 containing the gene for L-1 beta-lactamase from Pseudomonas maltophilia showed that this channel could also be used by SM-7338, Sch 33755, and Sch 33440 but apparently not by Sch 34343 or Sch 29482.
Trias
J
J
Department of Microbiology and Immunology, University of California, Berkeley 94720.
Nikaido
H
H
D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D002463:Cell Membrane Permeability; D004058:Diffusion; D015378:Imipenem / Q000494:pharmacology; D007700:Kinetics; D007769:Lactams; D000077731:Meropenem; D008970:Molecular Weight; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D013845:Thienamycins / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D007769:Lactams; D013845:Thienamycins; D015378:Imipenem; C034110:Sch 33755; C033922:Sch 29482; C063251:Sch 33440; C044617:Sch 34343; D000077731:Meropenem