Effect of tracer and intravenous fat emulsion on the measurement of reticuloendothelial system function.
Since the addition of lipid to intravenous feeding formulas, animal and human studies have shown impairment of the reticuloendothelial system (RES) due to slow rates of clearance and gradual accumulation of long chain triglycerides (LCT) in the liver. Medium chain triglycerides (MCT) accumulate only minimally in the liver and do not impair the RES. However, results from animal studies using technetium sulfur colloid (TSC) to assess RES function have been inconclusive. The present study reevaluates RES function after lipid infusion in guinea pigs as measured by organ distribution of TSC. Guinea pigs were fed 300 kcal/kg/day of total parenteral nutrition (TPN) for 2.5 days, with 50% of nonprotein calories as fat in the form of LCT or MCT, then injected intravenously with 2.5 or 25 microCi of TSC, and uptake by liver, spleen, and lungs was determined. Liver, lungs, and spleen all increased in size after TPN with LCT or MCT. Liver TSC uptake was significantly affected by the dose of TSC (p less than 0.05), with the high dose probably inducing an increased capacity of the liver to clear TSC from the blood. Liver uptake was not influenced by diet, but feeding MCT did significantly stimulate lung uptake of TSC (p less than 0.0001). This suggests that the hepatic TSC uptake system is not saturable, and may not be an appropriate measure of Kupffer cell function since the colloid is not phagocytosed. However, TSC blood clearance remains an excellent prognostic indicator for bacteremia and mortality in humans, and is useful for measuring global RES function.
Hirschberg
Y
Y
New England Deaconess Hospital, Laboratory of Nutrition/Infection, Boston, MA 02215.
Pomposelli
J J
JJ
Mascioli
E A
EA
Bistrian
B R
BR
Blackburn
G L
GL
D000818:Animals; D005217:Fat Emulsions, Intravenous / Q000493:pharmacokinetics*; D006168:Guinea Pigs; D008297:Male; D008657:Metabolic Clearance Rate; D012157:Mononuclear Phagocyte System / Q000187:drug effects* / Q000502:physiology; D010289:Parenteral Nutrition, Total / Q000379:methods; D013671:Technetium Tc 99m Sulfur Colloid
D005217:Fat Emulsions, Intravenous; D013671:Technetium Tc 99m Sulfur Colloid