Specific inhibition of iturin biosynthesis by cerulenin.
Cerulenin, an inhibitor of fatty acid synthesis, inhibits the biosynthesis of iturin by Bacillus subtilis. With a cerulenin concentration of 2 micrograms/mL, 50% inhibition was achieved. At this concentration, cerulenin does not affect growth or total protein synthesis but does inhibit the incorporation of sodium [14C]acetate, [14C]myristic acid, and [14C]asparagine into iturin. Since cerulenin is known to block the condensation of malonyl-CoA subunits in the formation of fatty acids, the inhibition of iturin and beta-amino acid syntheses by cerulenin is discussed in relation with lipid synthesis.
Hourdou
M L
ML
Laboratoire de biochimie microbienne, Université Claude Bernard, Lyon I, Villeurbanne, France.
Besson
F
F
Michel
G
G
D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D000935:Antifungal Agents / Q000494:pharmacology*; D001216:Asparagine / Q000378:metabolism; D001412:Bacillus subtilis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D002569:Cerulenin / Q000494:pharmacology*; D000431:Ethanol / Q000494:pharmacology; D005227:Fatty Acids / Q000096:biosynthesis; D050356:Lipid Metabolism; D008969:Molecular Sequence Data; D009227:Myristic Acids / Q000378:metabolism; D010455:Peptides; D010456:Peptides, Cyclic / Q000096:biosynthesis; D013997:Time Factors
D000085:Acetates; D000596:Amino Acids; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D005227:Fatty Acids; D009227:Myristic Acids; D010455:Peptides; D010456:Peptides, Cyclic; D002569:Cerulenin; D000431:Ethanol; C013579:iturin A; D001216:Asparagine; D019342:Acetic Acid