title: Comparative bacterial clearances of muscle and skin/subcutaneous tissues with and without dead bone: a laboratory study.
abstract: Seventeen New Zealand White rabbits underwent implantation of three different concentrations of bacteria and a sterile saline control solution with and without dead autologous bone in eight separate muscular and eight separate subcutaneous sites. Following a period of 1 week, each site was surgically explored and samples of tissue were taken for histology and quantitative culture. Results reveal that final bacterial concentrations in the subcutaneous sites were significantly lower than in the muscle sites (p less than or equal to 0.0001) for each concentration of bacteria, with and without dead bone. Dead bone resulted in very significantly greater bacterial concentrations in both subcutaneous and muscle sites. Clinically, these results indicate that a thorough bony wound debridement is more important than the type of tissue used to close the wound. Flap tissue should be selected with regard to the perfusion, contour, and appearance of the recipient site.
authors.affiliation: Division of Plastic and Reconstructive Surgery, Massachusetts General Hospital, Boston.
authors.forename: M
authors.initials: M
authors.lastname: Masem
authors.forename: B M
authors.initials: BM
authors.lastname: Greenberg
authors.forename: C
authors.initials: C
authors.lastname: Hoffman
authors.forename: D C
authors.initials: DC
authors.lastname: Hooper
authors.forename: J W
authors.initials: JW
authors.lastname: May
mesh_terms: D000038:Abscess / Q000473:pathology; D000818:Animals; D001842:Bone and Bones; D003872:Dermatitis / Q000473:pathology; D008214:Lymphocytes / Q000473:pathology; D009132:Muscles / Q000382:microbiology* / Q000473:pathology; D009220:Myositis / Q000473:pathology; D011817:Rabbits; D012867:Skin / Q000382:microbiology* / Q000473:pathology; D013203:Staphylococcal Infections; D013211:Staphylococcus aureus / Q000302:isolation & purification / Q000502:physiology*