title: Histopathology of melanocytic lesions in goats and establishment of a melanoma cell line: a potential model for human melanoma.
abstract: Melanocytic cells from white Angora goats were studied in vivo and in vitro. The histopathology of pigmented areas of skin from the most common sites of melanoma (solar-exposed areas of the ear, face, and perineum) resembled that of the epidermal melanocytes in Hutchinson's melanotic freckle in humans. Seven melanoma biopsies from 6 Angora goats showed histopathological features in common with human melanoma. A melanoma cell line, GM-1, was established in culture from a lymph node metastasis obtained from an animal that had a primary tumor excised and later developed extensive metastatic disease. GM-1 cells were mainly diploid, amelanotic, proliferated rapidly, spontaneously formed vacuolated cells, and were tumorigenic in nude mice. The species of origin of the GM-1 line was confirmed by isozyme profiles. GM-1 cultured cells and the original biopsy both expressed S-100 protein and tyrosinase antigen. Using GM-1 cells as the immunogen, a monoclonal antibody (MoAb 1F1) was derived that reacted strongly with a 116 kDa antigen in 50% of the GM-1 cells, but had little activity with goat fibroblasts (GM-F) or with human melanoma cells. GM-F, on the other hand, yielded more intense staining than GM-1 with an intermediate filament antibody (IFA), reacting with a 58 kDa antigen in both cell lines. The sensitivity of GM-1 to anticancer agents was similar to that of human melanoma cells. The pathology of caprine melanoma and its association with sun-exposed sites in relatively young animals suggest that it may be a suitable model for studying induction of melanoma by natural sunlight.
authors.affiliation: Queensland Institute of Medical Research, Herston, Brisbane, Australia.
authors.forename: P G
authors.initials: PG
authors.lastname: Parsons
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: J
authors.initials: J
authors.lastname: Candy
authors.forename: B
authors.initials: B
authors.lastname: Meyers
authors.forename: J
authors.initials: J
authors.lastname: Vickers
authors.forename: W R
authors.initials: WR
authors.lastname: Kelly
authors.forename: I
authors.initials: I
authors.lastname: Smith
authors.forename: P
authors.initials: P
authors.lastname: Spradbrow
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D014408:Biomarkers, Tumor; D002460:Cell Line; D004195:Disease Models, Animal; D005260:Female; D015511:Goat Diseases / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D006041:Goats; D008297:Male; D008544:Melanocytes / Q000473:pathology*; D008545:Melanoma / Q000737:chemistry / Q000209:etiology / Q000473:pathology / Q000662:veterinary*; D051379:Mice; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000032:analysis; D009368:Neoplasm Transplantation; D012878:Skin Neoplasms / Q000737:chemistry / Q000209:etiology / Q000473:pathology / Q000662:veterinary*; D013472:Sunlight / Q000009:adverse effects; D014183:Transplantation, Heterologous
chemical_list: D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins