Characterization of murine liver-derived inhibitory protein.
Murine liver-derived inhibitory protein (LIP) capable of inhibiting human lymphocyte proliferation was highly purified from liver extract. Its molecular weight determined by gel filtration and SDS-PAGE was 105,000 and 38,400 respectively. LIP moved electrophoretically at the gamma-globulin region. Its activity in inhibiting lymphocyte proliferation was temperature-stable up to 60 degrees C, and pH-stable between 4 and 11. It was not cytotoxic to lymphocytes as shown in 51Cr-release experiments. The purified LIP possessed arginase activity.
Wang
S R
SR
Department of Medicine, Veterans General Hospital, Taiwan, ROC.
Huang
M H
MH
Chang
K L
KL
Yu
C L
CL
Chiang
B N
BN
Han
S H
SH
D000818:Animals; D001119:Arginase / Q000378:metabolism; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000502:physiology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008813:Mice, Inbred ICR; D008970:Molecular Weight; D011489:Protein Denaturation; D011506:Proteins / Q000302:isolation & purification / Q000494:pharmacology / Q000502:physiology*
D011506:Proteins; D001119:Arginase