The promoter of the glucoamylase-encoding gene of Aspergillus niger functions in Ustilago maydis.
Promoter sequences from the Aspergillus niger glucoamylase-encoding gene (glaA) were linked to the bacterial hygromycin (Hy) phosphotransferase-encoding gene (hph) and this chimeric marker was used to select Hy-resistant (HyR) Ustilago maydis transformants. This is an example of an Ascomycete promoter functioning in a Basidiomycete. HyR transformants varied with respect to copy number of integrated vector, mitotic stability, and tolerance to Hy. Only 216 bp of glaA promoter sequence is required for expression in U. maydis but this promoter is not induced by starch as it is in Aspergillus spp. The transcriptional start points are the same in U. maydis and A. niger.
Smith
T L
TL
Institute for Microbial and Biochemical Technology, U.S. Department of Agriculture, Madison, WI 53705.
Gaskell
J
J
Berka
R M
RM
Yang
M
M
Henner
D J
DJ
Cullen
D
D
D001234:Aspergillus niger / Q000235:genetics*; D001487:Basidiomycota / Q000235:genetics*; D002934:Cinnamates; D004274:DNA, Recombinant; D004352:Drug Resistance, Microbial / Q000235:genetics; D005087:Glucan 1,4-alpha-Glucosidase / Q000235:genetics*; D006921:Hygromycin B / Q000031:analogs & derivatives; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic / Q000502:physiology*; D014170:Transformation, Genetic; D014588:Ustilago / Q000235:genetics*
D002934:Cinnamates; D004274:DNA, Recombinant; D006921:Hygromycin B; C026273:hygromycin A; D005087:Glucan 1,4-alpha-Glucosidase