title: Comparison of persistence of seven bovine viruses on bovine embryos following in vitro exposure.
abstract: The ability of seven cytopathic strains of bovine viruses to adhere to the zona pellucida of six-to-eight day-old bovine embryos were compared. Embryos were exposed to virus by placing them either in virus suspensions or by culturing them on infected bovine turbinate cultures for 18-24 h. After exposure to bovine virus diarrhea virus (BVDV), infectious bovine rhinotracheitis virus (IBV), bluetongue virus (BTV), pseudorabies virus (PRV), vesicular stomatitis virus (VSV), parainfluenza 3 virus (PI3), or bovine enterovirus virus (BEV), the embryos were tested for virus by culture in bovine turbinate cells and by morphological examination using electron microscopy (EM). A special technique to minimize loss of embryos processed for EM was developed. More embryos had viral particles on the surface of the zona pellucida after exposure to 18-24 hour infected cell cultures than did embryos exposed to viral culture suspensions. The most dramatic finding was that BTV adhered in large numbers to the surface of the zona pellucida of exposed embryos. IBRV, PRV, and VSV comprised an intermediate group, with virions occasionally detected on the surface of exposed embryos after 5 washes. Therefore, extensive washing is required. The PI3 and BEV were easily removed from embryo-exposed virus by washing. BVD was difficult to identify morphologically, making assessment by EM unreliable. There was no evidence that any one of the seven viruses penetrated the intact zona pellucida. Using a micromanipulator, 42 embryos were also directly inoculated through the zona pellucida with +/- 50 picoliters of virus inoculum or medium.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Microbiology, Immunology and Parasitology, New York State College of Agriculture and Life Sciences, Cornell University.
authors.forename: J H
authors.initials: JH
authors.lastname: Gillespie
authors.forename: D H
authors.initials: DH
authors.lastname: Schlafer
authors.forename: R H
authors.initials: RH
authors.lastname: Foote
authors.forename: S
authors.initials: S
authors.lastname: Quick
authors.forename: E
authors.initials: E
authors.lastname: Dougherty
authors.forename: E
authors.initials: E
authors.lastname: Schiff
authors.forename: S
authors.initials: S
authors.lastname: Allen
mesh_terms: D000818:Animals; D002417:Cattle / Q000196:embryology*; D004624:Embryo Transfer / Q000662:veterinary; D066298:In Vitro Techniques; D008845:Microinjections; D008854:Microscopy, Electron; D010063:Ovum / Q000382:microbiology*; D018406:Virus Physiological Phenomena; D015044:Zona Pellucida / Q000382:microbiology*