Tetravalent vanadium mediated oxidation of low density lipoprotein.
1. Tetravalent vanadium causes oxidation of low density lipoprotein (LDL) as manifest by protein degradation and lipid peroxidation. 2. Oxidative modification of the apolipoprotein B-100 is paralleled by the formation of thiobarbituric acid reactive substance and fluorescent chromolipid production. 3. The metal chelators ethylenediamine tetracetic acid and desferrioxamine, and the alcohols, ethanol and isopropanol inhibit the oxidation of LDL by tetravalent vanadium. No inhibition is observed with superoxide dismutase, catalase or mannitol. 4. The data suggest that aldehydes formed during the process of lipid peroxidation induced by tetravalent vanadium react with the proteins in LDL to form fluorescent chromolipids and that the oxidative process originates within the hydrophobic domain of LDL.
Dickson
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New York University Medical Center, Department of Pharmacology, NY 10016.
Stern
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D000433:1-Propanol / Q000494:pharmacology; D002412:Cations; D003676:Deferoxamine / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D000431:Ethanol / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000378:metabolism*; D010084:Oxidation-Reduction; D013050:Spectrometry, Fluorescence; D013858:Thiobarbiturates; D014639:Vanadium / Q000494:pharmacology*; D017968:Vanadium Compounds
D002412:Cations; D008077:Lipoproteins, LDL; D013858:Thiobarbiturates; D017968:Vanadium Compounds; D014639:Vanadium; D000431:Ethanol; C034028:vanadyl sulfate; D000433:1-Propanol; D004492:Edetic Acid; D003676:Deferoxamine; C029684:thiobarbituric acid