title: Production of arachidonic and linoleic acid metabolites by guinea pig tracheal epithelial cells.
abstract: Pulmonary epithelial cells may be responsible for regulating airway smooth muscle function, in part by release of fatty acid-derived mediators. Incubation of isolated guinea pig tracheal epithelial cells with radiolabeled arachidonic acid (AA) leads to the production of 5- and 15-hydroxyeicosatetraenoic acid (5- and 15-HETE) and smaller amounts of leukotriene (LT) B4 and C4 and 12-hydroxyheptadecatrienoic acid (HHT). Epithelial cells also are able to release linoleic acid (LA) metabolites. Incubation with radiolabeled linoleic acid leads to the formation of 9- and 13-hydroxyoctadecadienoic acid (9- and 13-HODE). The biological significance of these mediators produced by epithelial cells is discussed.
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, State University of Utrecht, The Netherlands.
authors.forename: M J
authors.initials: MJ
authors.lastname: Oosthuizen
authors.forename: F
authors.initials: F
authors.lastname: Engels
authors.forename: B
authors.initials: B
authors.lastname: Van Esch
authors.forename: P A
authors.initials: PA
authors.lastname: Henricks
authors.forename: F P
authors.initials: FP
authors.lastname: Nijkamp
mesh_terms: D000818:Animals; D000991:Antithrombins / Q000096:biosynthesis; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D002250:Carbon Radioisotopes; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D006168:Guinea Pigs; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis; D008041:Linoleic Acids / Q000096:biosynthesis / Q000378:metabolism*; D044243:Linoleic Acids, Conjugated; D008297:Male; D010477:Perfusion; D014132:Trachea / Q000378:metabolism*
chemical_list: D000991:Antithrombins; D001095:Arachidonic Acids; D002250:Carbon Radioisotopes; D005227:Fatty Acids; D006893:Hydroxyeicosatetraenoic Acids; D008041:Linoleic Acids; D044243:Linoleic Acids, Conjugated; C024347:9-hydroxy-10,12-octadecadienoic acid; D016718:Arachidonic Acid; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; C024348:13-hydroxy-9,11-octadecadienoic acid; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid