Distribution and scatter of yeast cell phagocytosis by human monocytes in an improved glass surface assay.
The assessment of yeast cell phagocytosis by glass-adherent monocytes was improved by ultrasonication of yeast cells prior to the experiments in order to prevent aggregation, restriction of measurements to completed engulfment regardless of the number of peptides ingested, and stopping of phagocytosis before counting by maintained cooling and the addition of EDTA. The modifications provided a reduced dispersion of individual values, increased engulfment of yeast cells, and decreased numbers of yeast cells only adherent to the monocyte membrane. The improvement made the method more convenient for the study of engulfment by monocytes.
Bjermer
L
L
Department of Lung Medicine, University Hospital, Umeå, Sweden.
Athlin
L
L
Sondell
K
K
Eriksson
S
S
Norberg
B
B
D005898:Glass; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology*; D012441:Saccharomyces cerevisiae / Q000276:immunology*; D013499:Surface Properties