[Phenotypic and functional characterization of cloned T-lymphocytes from cerebrospinal fluid of patients with multiple sclerosis].
T cell clones derived from the cerebrospinal fluid of patients with multiple sclerosis were investigated for their ability to produce IL2, IL4, IFN gamma and TNF alpha. As controls, liver infiltrating T lymphocyte clones from patients with chronic active hepatitis were used. All CSF clones (both CD4+ and CD8+) produced high amounts of IFN gamma and particularly of TNF alpha. TNF was synthesized in a significantly higher amount than control clones. Moreover, they were capable of secreting IL2 but not IL4. From our results we conclude that CSF-CD4+ T clones could constitute a subset with functional properties similar to those of the Th1/inflammatory cells of the mouse. The unusually high amount of TNF produced by CSF derived T cell clones strongly suggests a significant role for this cytokine in MS immunopathogenesis.
Barnaba
V
V
Fondazione Andrea Cesalpino, I Clinica Medica, Università La Sapienza, Roma.
Benvenuto
R
R
Buttinelli
C
C
Salvetti
M
M
Toma
L
L
Fiori
P
P
D000328:Adult; D002455:Cell Division; D002555:Cerebrospinal Fluid / Q000166:cytology; D002999:Clone Cells; D016207:Cytokines / Q000378:metabolism; D003601:Cytotoxicity Tests, Immunologic; D006801:Humans; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid*; D010641:Phenotype; D013601:T-Lymphocytes / Q000502:physiology*
D016207:Cytokines
Caratterizzazione fenotipica e funzionale di cloni T linfocitari di derivazione liquorale in pazienti affetti da sclerosi multipla.