title: Lack of regio- and stereospecificity in oxidation of (+) camphor by Streptomyces griseus enriched in cytochrome P-450soy.
abstract: Oxidation of (+) camphor by cytochrome P-450soy-enriched intact cells of Streptomyces griseus resulted in the formation of one major and several minor metabolites. The minor metabolites were identified as 3-endo-hydroxycamphor (2%), 5-endo-hydroxycamphor (7%), 5-exo-hydroxycamphor (9%), 2,5-diketobornane (2%), and camphorquinone (3%). The major metabolite was isolated and conclusively identified as 6-endo-hydroxycamphor (60%). When supplemented with NADPH, spinach ferredoxin:NADP oxidoreductase and spinach ferredoxin, homogeneous preparations of cytochrome P-450soy oxidized camphor to a mixture of 3-endo-, 5-endo-, 5-exo-and 6-endo-hydroxycamphor. The data presented indicates that cytochrome P-450soy resembles its mammalian counterparts in its lack of regio- and stereospecificity in camphor oxidation.
authors.affiliation: Central Research and Development Department, E. I. duPont de Nemours & Company, Wilmington, DE 19880-0228.
authors.forename: F S
authors.initials: FS
authors.lastname: Sariaslani
authors.forename: L R
authors.initials: LR
authors.lastname: McGee
authors.forename: M K
authors.initials: MK
authors.lastname: Trower
authors.forename: F G
authors.initials: FG
authors.lastname: Kitson
mesh_terms: D002164:Camphor / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005433:Flour; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D013025:Glycine max / Q000201:enzymology; D013237:Stereoisomerism; D013305:Streptomyces griseus / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*
chemical_list: D002164:Camphor; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; C028878:ferredoxin-NAD+ reductase