title: A lipopeptide-encoding sequence upstream from the lysA gene of Pseudomonas aeruginosa.
abstract: An open reading frame (ORF) of 141 bp was observed upstream from the Pseudomonas aeruginosa lysA gene. The translation product of this ORF contains a signal peptide with a lipoprotein box, Ile-Ala-Ala-Cys, at the predicted signal peptidase cleavage site. The Escherichia coli phoA gene without its signal sequence was fused in frame to this ORF in a broad host-range plasmid. The resulting construct expressed a hybrid protein exhibiting alkaline phosphatase activity in phoA mutants of both E. coli and P. aeruginosa. This indicates that the ORF encodes a peptide, part of which acts as an export signal. The hybrid peptide was identified by immunoblotting with alkaline phosphatase antiserum. The accumulation of a precursor form was observed when P. aeruginosa cells carrying this gene fusion on a plasmid were treated with globomycin. Moreover, the mature form could be labelled with 2-[3H]-glycerol, indicating that lipidic residues may be linked to the hybrid protein. Taken together, these results strongly suggest that the ORF encodes a lipopeptide. We propose that the gene is called IppL.
authors.affiliation: Laboratoire de Chimie Bactérienne, C.N.R.S., Marseille, France.
authors.forename: A
authors.initials: A
authors.lastname: Jann
authors.forename: D
authors.initials: D
authors.lastname: Cavard
authors.forename: C
authors.initials: C
authors.lastname: Martin
authors.forename: B
authors.initials: B
authors.lastname: Cami
authors.forename: J C
authors.initials: JC
authors.lastname: Patte
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003062:Codon; D015870:Gene Expression; D005798:Genes, Bacterial; D008074:Lipoproteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000254:growth & development; D012333:RNA, Messenger; D015183:Restriction Mapping
chemical_list: D003062:Codon; D008074:Lipoproteins; D021382:Protein Sorting Signals; D012333:RNA, Messenger