An abnormal profile of DNA replication intermediates in Bloom's syndrome.
Bloom's syndrome (BS) cells display a characteristic genomic instability, notably an elevated frequency of sister-chromatid exchange. Replicating DNA in cultured BS cells was labeled with [3H]thymidine using several time schedules. Separation of DNA in agarose gels showed high molecular weight DNA and three classes of DNA replication intermediates: 20-kilobase DNA, 10-kilobase DNA, and Okazaki fragments. In contrast newly replicated DNA from normal cells showed no 20-kilobase DNA replication intermediates. Certain BS cells, exceptional in that their characteristic genomic instability has for unknown reasons been corrected, also differed from normal cells in having the 20-kilobase intermediate, but they differed from both normal cells and the other (the uncorrected) BS cells in lacking the 10-kilobase DNA replication intermediates.
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Radiumhemmet, Karolinska Hospital, Stockholm, Sweden.
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Nylen
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Winblad
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German
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D016590:Aphidicolin; D001816:Bloom Syndrome / Q000235:genetics*; D002460:Cell Line; D004261:DNA Replication; D004224:Diterpenes; D006801:Humans; D008970:Molecular Weight; D012854:Sister Chromatid Exchange; D013997:Time Factors
D004224:Diterpenes; D016590:Aphidicolin