title: Expression of autoantibodies to recombinant (U1) RNP-associated 70K antigen in systemic lupus erythematosus.
abstract: To determine the specificity of antibodies to the (U1) ribonucleoprotein antigen in systemic lupus erythematosus (SLE), patient sera were tested for binding to a recombinant human 70K antigen. By solid-phase immunoassay, we detected anti-70K reactivity in sera from 31 of 96 patients with systemic lupus erythematosus (SLE), demonstrating that anti-70K antibodies may occur in patients with SLE as well as other clinical diagnoses. In sequential sera from 2 of these patients, we found that anti-70K binding varied dramatically over the course of disease. The changes in anti-70K antibody levels did not correlate with clinical events nor evolving antibody reactivity with the Sm-specific antigens.
authors.affiliation: Department of Medicine, Duke University Medical Center, Durham, North Carolina.
authors.forename: E W
authors.initials: EW
authors.lastname: St Clair
authors.forename: C C
authors.initials: CC
authors.lastname: Query
authors.forename: R
authors.initials: R
authors.lastname: Bentley
authors.forename: J D
authors.initials: JD
authors.lastname: Keene
authors.forename: R P
authors.initials: RP
authors.lastname: Polisson
authors.forename: N B
authors.initials: NB
authors.lastname: Allen
authors.forename: D S
authors.initials: DS
authors.lastname: Caldwell
authors.forename: J R
authors.initials: JR
authors.lastname: Rice
authors.forename: C
authors.initials: C
authors.lastname: Cox
authors.forename: D S
authors.initials: DS
authors.lastname: Pisetsky
mesh_terms: D001323:Autoantibodies / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D015153:Blotting, Western; D003240:Connective Tissue Diseases / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D005779:Immunodiffusion; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D012342:RNA, Small Nuclear; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012261:Ribonucleoproteins / Q000276:immunology*; D055517:snRNP Core Proteins
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D012342:RNA, Small Nuclear; D011993:Recombinant Fusion Proteins; D012261:Ribonucleoproteins; D055517:snRNP Core Proteins