The effect of mono(2-ethylhexyl) phthalate on Sertoli cell transferrin secretion in vitro.
We have examined the effect of mono(2-ethylhexyl) phthalate (MEHP) on the secretion of rat transferrin (rTf) by Sertoli cells (SC) in the presence and absence of FSH. Significant decreases in rTf secretion were observed in SC cultures exposed to 50 and 75 microM MEHP, in the absence of FSH. Treatment of SC with FSH (300 ng/ml) obviated this suppression of rTf secretion by MEHP. These findings indicate an effect of MEHP on rTf secretion by SC in vitro which could account for the testicular toxicity of MEHP in vivo.
Thysen
B
B
Department of Laboratory Medicine, Albert Einstein College of Medicine, Yeshiva University, Bronx, New York 10461.
Morris
P L
PL
Gatz
M
M
Bloch
E
E
D000818:Animals; D002478:Cells, Cultured; D004051:Diethylhexyl Phthalate / Q000633:toxicity / Q000031:analogs & derivatives; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012708:Sertoli Cells / Q000187:drug effects* / Q000378:metabolism; D014168:Transferrin / Q000378:metabolism*
D014168:Transferrin; D005640:Follicle Stimulating Hormone; D004051:Diethylhexyl Phthalate; C016599:mono-(2-ethylhexyl)phthalate