title: The effect of pharmacological modification of gastric emptying and mouth-to-caecum transit time on the absorption of sugar probe marker molecules of intestinal permeability in normal man.
abstract: The present study examined the hypothesis that altered motility of the gastrointestinal tract affects absorption of probe markers of intestinal permeability. Seven healthy subjects, aged 32-44 years, received saline, 600 micrograms atropine or 10 mg metoclopramide in randomized order at weekly intervals. After 10 min they ingested a test solution containing 5 g lactulose, 5 g mannitol and 2 g 3-O-methyl glucose in 100 ml tap water. The molarity of the solution was 542 mmol l-1 and the dose administered was 80 ml m-2 body surface area. Gastric emptying was measured by ultrasound, mouth-to-caecum transit time by breath hydrogen analysis and sugar concentrations by gas-liquid chromatography. Gastric emptying half-times (min) were [mean (95% confidence intervals)] 14.9 (11:4-18.5) after saline, 22 (18.7-25.2) after atropine and 10.3 (7.0-12.6) after metoclopramide (P less than 0.002). Transit times (min) were 68.9 (52-85.2) after saline, 143 (126-159) after atropine and 38 (21.2-54.5) after metoclopramide; P less than 0.0001. Analysis of plasma levels of mannitol and 3-O-methyl glucose showed a significant within-subject effect of drug with time (P less than 0.03). Urinary excretion of mannitol in the first 5 h after ingestion of the test solution was 1256 (974-1620) mg after saline, 1560 (1210-2013) mg after atropine and 955 (740-1232) mg after metoclopramide (P less than 0.03). There were no significant differences in lactulose and 3-O-methyl glucose urinary excretion between drug treatments.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Child Health, University of Newcastle upon Tyne, Medical School, UK.
authors.forename: A L
authors.initials: AL
authors.lastname: Brunetto
authors.forename: A D
authors.initials: AD
authors.lastname: Pearson
authors.forename: R
authors.initials: R
authors.lastname: Gibson
authors.forename: D N
authors.initials: DN
authors.lastname: Bateman
authors.forename: M U
authors.initials: MU
authors.lastname: Rashid
authors.forename: M F
authors.initials: MF
authors.lastname: Laker
mesh_terms: D000328:Adult; D001285:Atropine / Q000494:pharmacology; D002241:Carbohydrates / Q000493:pharmacokinetics; D005746:Gastric Emptying / Q000187:drug effects / Q000502:physiology*; D005772:Gastrointestinal Transit / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007408:Intestinal Absorption / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008787:Metoclopramide / Q000494:pharmacology; D010539:Permeability
chemical_list: D002241:Carbohydrates; D001285:Atropine; D008787:Metoclopramide