title: Fluctuations of euglobulin lysis time, tissue plasminogen activator, and free and total plasminogen activator inhibitor levels in plasma in daytime.
abstract: Blood was taken from healthy 15 males and 10 females at 9:30 h, 10:00 h and 12:30 h. The euglobulin fraction was prepared and clotted by the addition of human thrombin. The clot lysis time shortened significantly from 9:30 h to 10:00 h (p less than 0.01) and further to 12:30 h (p less than 0.01). Plasma levels of tissue plasminogen activator (t-PA) antigens did not change from 9:30 h to 10:00 h, but slightly and significantly to 12:30 h (p less than 0.01). Plasma levels of free plasminogen activator inhibitor-1 (PAI-1) and complex of t-PA-PAI-1 decreased from 9:30 h to 10:00 h (p less than 0.02) and to 12:30 h (p less than 0.01). Plasma levels of total PAI-1 (free plus complex) decreased from 9:30 h to 10:00 h (p less than 0.01) and to 12:30 h (p less than 0.01). These results suggest that a major factor contributing to the enhanced fibrinolytic activity of the euglobulin fraction may be a level of PAI-1 (free and total).
authors.affiliation: Department of Physiology, Hamamatsu University, School of Medicine, Shizuoka-ken, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Takada
authors.forename: Y
authors.initials: Y
authors.lastname: Takada
authors.forename: T
authors.initials: T
authors.lastname: Urano
authors.forename: K
authors.initials: K
authors.lastname: Sakakibara
authors.forename: A
authors.initials: A
authors.lastname: Rydzewski
mesh_terms: D000328:Adult; D001777:Blood Coagulation / Q000187:drug effects / Q000502:physiology; D002940:Circadian Rhythm; D005260:Female; D005342:Fibrinolysis / Q000187:drug effects / Q000502:physiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D015849:Plasminogen Inactivators / Q000097:blood* / Q000494:pharmacology; D012712:Serum Globulins / Q000378:metabolism / Q000502:physiology*; D010959:Tissue Plasminogen Activator / Q000097:blood*
chemical_list: D015849:Plasminogen Inactivators; D012712:Serum Globulins; D010959:Tissue Plasminogen Activator