Comparison of the mode of action of ras p21 with those of protein kinases A and C in the stimulation of gene expression in NIH/3T3 cells.
To compare the mode of action of ras p21 with those of protein kinases A and C in the regulation of gene expression in NIH/3T3 cells, we investigated the transcriptional activity of various enhancer/promoters and enhancer motifs in the cells transfected with the c-Ha-rasva112 complementary DNA (cDNA). The results indicate that the c-Ha-rasva112 protein stimulates the enhancer/promoters of the c-fos gene, the metallothionein IIA gene, the simian virus 40 (SV40) virus genome and the Rous sarcoma (RS) virus genome, and the serum-response element and the 12-O-tetradecanoylphorbol-13-acetate (TPA)-response element in a manner independent of protein kinases A and C in NIH/3T3 cells.
Oku
N
N
Department of Biochemistry, Kobe University School of Medicine, Japan.
Kaibuchi
K
K
Fukumoto
Y
Y
Hori
Y
Y
Fujioka
H
H
Takai
Y
Y
D000818:Animals; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D008156:Luciferases / Q000235:genetics; D008668:Metallothionein / Q000235:genetics; D051379:Mice; D015689:Oncogene Protein p21(ras) / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011493:Protein Kinase C / Q000502:physiology*; D011494:Protein Kinases / Q000502:physiology*; D011518:Proto-Oncogene Proteins / Q000235:genetics; D016760:Proto-Oncogene Proteins c-fos; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D014162:Transfection
D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D011993:Recombinant Fusion Proteins; D008668:Metallothionein; D008156:Luciferases; D015500:Chloramphenicol O-Acetyltransferase; D011494:Protein Kinases; D011493:Protein Kinase C; D015689:Oncogene Protein p21(ras)