title: Evaluation of Bacto TB hydrolysis reagent (Tween 80) for the identification of Branhamella catarrhalis.
abstract: An investigation of the hydrolysis of Tween 80 reagent by Branhamella catarrhalis and related organisms (Neisseria and Moraxella species) revealed that only B. catarrhalis gave a positive result. A total of 226 strains, including reference organisms and clinical isolates, were studied. B. catarrhalis changed the color of the reagent from amber to pink-red after overnight incubation. We recommend this simple and cost-effective test as an alternative procedure to DNase testing or tributyrin hydrolysis or as a supplemental procedure for the identification of B. catarrhalis in clinical specimens.
authors.affiliation: Department of Pathology, Methodist Hospital of Brooklyn, New York 11215.
authors.forename: M
authors.initials: M
authors.lastname: Weiner
authors.forename: P D
authors.initials: PD
authors.lastname: Penha
mesh_terms: D001431:Bacteriological Techniques; D005069:Evaluation Studies as Topic; D006801:Humans; D006868:Hydrolysis; D007202:Indicators and Reagents; D001936:Moraxella catarrhalis / Q000302:isolation & purification*; D011136:Polysorbates
chemical_list: D007202:Indicators and Reagents; D011136:Polysorbates