[Use of a lysoenzyme preparation from Streptomyces recifensis subsp. lyticus 2435 for isolating DNA from staphylococcal cells].
It was shown that the preparation 2435 from Streptomyces recifensis subsp. lyticus, including a complex of bacteriolytic and concomitant enzymes provided lysis of thick staphylococcal suspensions within 15 to 20 minutes under optimal conditions after preliminary treatment of the cells with 0.1 M cystein-HCl. A procedure was developed for isolating DNA from the cells of staphylococci and other microorganisms based on enzymatic lysis. In terms of major physicochemical properties, the preparations of DNA were not inferior to the preparations of DNA isolated by the classical Marmur technique with Kirbi's deproteinization and had transforming activity. The developed procedure for isolation of DNA with using the lysoenzyme preparation widened the possibilities of investigating the genetics of staphylococci and other microorganisms.
Babenko
Iu S
IuS
Oriabinskaia
L B
LB
D001433:Bacteriolysis / Q000187:drug effects*; D003470:Culture Media; D004269:DNA, Bacterial / Q000187:drug effects / Q000302:isolation & purification*; D004492:Edetic Acid / Q000494:pharmacology; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D009113:Muramidase / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000166:cytology / Q000187:drug effects / Q000235:genetics*; D013291:Streptococcus / Q000201:enzymology*; D014020:Tissue Extracts / Q000494:pharmacology
D003470:Culture Media; D004269:DNA, Bacterial; D007202:Indicators and Reagents; D014020:Tissue Extracts; D012965:Sodium Chloride; D004492:Edetic Acid; D009113:Muramidase
Primenenie lizoénzimnogo preparata Streptomyces recifensis subsp. lyticus 2435 dlia vydeleniia DNK iz kletok stafilokokka.