title: Evaluation of an enzyme-linked immunosorbent assay that uses ferrous metal beads for determination of antihistoplasmal immunoglobulins G and M.
abstract: A rapid enzyme-linked immunosorbent assay (ELISA) for the detection of human class-specific antibodies to Histoplasma capsulatum (histoplasmal immunoglobulin M [HIgM] and histoplasmal IgG [HIgG]) was developed by using antigen adsorbed onto polycarbonate-coated ferrous beads. In the ELISA method all the reagents used were commercially available. In 135 specimens from patients with confirmed histoplasmosis, sensitivities were 76% for complement fixation (CF), 53% for immunodiffusion (ID), and 64% for the ELISA for HIgM and HIgG combined. The ELISA detected histoplasmal antibody in 36% of the specimens with negative antibody titers by CF and 46% of the specimens with negative antibody titers by ID. The ELISA detected histoplasmal antibody in 27% of specimens that were negative by both CF and ID. When limited to specimens collected within 4 months of the onset of histoplasmosis symptoms, sensitivities were 82% for CF, 63% for ID, and 86% for ELISA for HIgG and HIgM combined. Within this group, ELISA detected histoplasmal antibody in 90% of the specimens that were negative by CF, 76% that were negative by ID, and 100% that were negative by both CF and ID. The specificity of the ELISA could not be fully addressed since sera from patients with other fungal infections were not available.
authors.affiliation: Department of Pathology, Indiana University School of Medicine, Indianapolis 46202.
authors.forename: S E
authors.initials: SE
authors.lastname: Zimmerman
authors.forename: M L
authors.initials: ML
authors.lastname: French
authors.forename: M B
authors.initials: MB
authors.lastname: Kleiman
authors.forename: L J
authors.initials: LJ
authors.lastname: Wheat
mesh_terms: D000908:Antibodies, Fungal / Q000032:analysis*; D003168:Complement Fixation Tests; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006658:Histoplasma / Q000276:immunology*; D006660:Histoplasmosis / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007501:Iron
chemical_list: D000908:Antibodies, Fungal; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007501:Iron