A simple, general method for detecting retroviral RNAs expressed in cells.
A simple, efficient method has been developed for detecting retroviral RNAs expressed in cells. In this method, total RNAs or poly A+ RNAs extracted from various human cells are separated by electrophoresis and hybridized with synthetic oligonucleotides corresponding to the 3'-terminal 18 nucleotides of various tRNAs. Genomic and subgenomic RNAs of HTLV-I and HTLV-II in virus-infected cells and of xenotropic murine leukemia virus expressed in human lung cancer cells were easily detected with the tRNA(Pro)-derived oligonucleotide probe. This technique can be used to search for unidentified retroviruses expressed in human cancer cells and tissues.
Harada
F
F
Department of Biophysics, Kanazawa University.
Hirose
Y
Y
Tanaka
M
M
Sasaki
T
T
Kamiya
H
H
Miura
K
K
D000818:Animals; D001402:B-Lymphocytes / Q000382:microbiology; D001483:Base Sequence; D003001:Cloning, Molecular; D006367:HeLa Cells; D015368:Human T-lymphotropic virus 1 / Q000235:genetics; D015367:Human T-lymphotropic virus 2 / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009368:Neoplasm Transplantation; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D012343:RNA, Transfer; D012361:RNA, Transfer, Pro; D012367:RNA, Viral / Q000032:analysis*; D012091:Repetitive Sequences, Nucleic Acid; D012190:Retroviridae / Q000235:genetics*; D013601:T-Lymphocytes / Q000382:microbiology; D014407:Tumor Cells, Cultured
D015345:Oligonucleotide Probes; D012361:RNA, Transfer, Pro; D012367:RNA, Viral; D012343:RNA, Transfer