[Functional alterations of human platelets following 111In labeling with different ligands and incubation media].
We studied the effects of various 111In-water soluble chelates and incubation media on labeling efficiency of platelets and in vitro platelet aggregability. High labeling efficiency of platelets in ACD-saline was achieved with 111In-oxine sulfate, 111In-tropolone and 111In-MPO (2-mercaptopyridine-N-oxide). In the condition with 4.8 x 10(6)/mm3 platelets in ACD-plasma, 111In-oxine-sulfate had low labeling efficiency and inconsistent labeling, while 111In-tropolone and 111In-MPO had high labeling efficiency. In vitro platelet aggregability (ADP 2 microM) was reduced when platelets were labeled in the absence of plasma. However, there was no significant difference in platelet aggregability among 111In-platelets labeled by three different chelates. In conclusion, to maintain aggregation activity of the platelets with relatively high labeling efficiency, the best result was obtained by using MPO or tropolone chelate in plasma at 4.8 x 10(6)/microliters platelet concentration.
Mieno
M
M
Division of Nuclear Medicine, Osaka University Medical School.
Isaka
Y
Y
Kimura
K
K
Matsumoto
M
M
Etani
H
H
Uehara
A
A
Hashikawa
K
K
Hata
R
R
Moriwaki
H
H
Ashida
K
K
D000328:Adult; D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology; D019343:Citric Acid; D003470:Culture Media; D003508:Cycloheptanes / Q000494:pharmacology*; D005947:Glucose / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D006912:Hydroxyquinolines / Q000494:pharmacology*; D007205:Indium Radioisotopes; D008024:Ligands; D008297:Male; D015125:Oxyquinoline / Q000494:pharmacology*; D010949:Plasma; D010974:Platelet Aggregation / Q000187:drug effects; D011725:Pyridines / Q000494:pharmacology*; D013871:Thiones; D014334:Tropolone / Q000494:pharmacology*
D003470:Culture Media; D003508:Cycloheptanes; D006912:Hydroxyquinolines; D007205:Indium Radioisotopes; D008024:Ligands; D011725:Pyridines; D013871:Thiones; C002113:acid citrate dextrose; D019343:Citric Acid; D015125:Oxyquinoline; C008704:pyrithione; D014334:Tropolone; D005947:Glucose