title: A comparison of the effects of three positive inotropic agents (amrinone, milrinone and medorinone) on platelet aggregation in human whole blood.
abstract: Amrinone, milrinone and medorinone inhibit platelet aggregation in human whole blood. They are particularly potent inhibitors of arachidonic acid induced aggregation, inhibiting by 50% (IC50) at concentrations of 1.5 microM (milrinone), 7.5 microM (medorinone) and 48 microM (amrinone). Each drug was less potent at inhibiting ADP and collagen-induced aggregation. The rank order for inhibition of arachidonic acid - induced aggregation correlated well with the rank order of cyclic AMP phosphodiesterase inhibition for these drugs when compared to the response of a reference cAMP phosphodiesterase inhibitor (CI-930) and a reference cGMP phosphodiesterase inhibitor (M & B 22948). Since inhibition of platelet aggregation in vitro occurred at clinically relevant concentrations, it is evident that these agents have potentially beneficial antithrombotic properties.
authors.affiliation: Sterling Winthrop Research Centre, Alnwick, Northumberland, United Kingdom.
authors.forename: A
authors.initials: A
authors.lastname: Pattison
authors.forename: N
authors.initials: N
authors.lastname: Astley
authors.forename: C T
authors.initials: CT
authors.lastname: Eason
authors.forename: F W
authors.initials: FW
authors.lastname: Bonner
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000638:Amiodarone / Q000494:pharmacology*; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D002316:Cardiotonic Agents / Q000494:pharmacology*; D003094:Collagen / Q000494:pharmacology; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D006801:Humans; D008297:Male; D020105:Milrinone; D009287:Naphthyridines / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011724:Pyridazines / Q000494:pharmacology; D011728:Pyridones / Q000494:pharmacology*
chemical_list: D001095:Arachidonic Acids; D002316:Cardiotonic Agents; D005343:Fibrinolytic Agents; D009287:Naphthyridines; D010975:Platelet Aggregation Inhibitors; D011724:Pyridazines; D011728:Pyridones; D016718:Arachidonic Acid; D000244:Adenosine Diphosphate; C064867:medorinone; C042528:4,5-dihydro-6-(4-(imidazol-1-yl)phenyl)-5-methyl-3(2H)-pyridazinone; D003094:Collagen; D020105:Milrinone; D000638:Amiodarone