title: Enzyme-linked immunosorbent assay for distinguishing serological responses of lepromatous and tuberculoid leprosies to the 29/33-kilodalton doublet and 64-kilodalton antigens of Mycobacterium tuberculosis.
abstract: Immunoblot assays for the antibodies to Mycobacterium tuberculosis sonic extracts showed that all serum specimens of 40 lepromatous and of 28 tuberculoid leprosy patients reacted in a significant manner to 29/33-kilodalton (kDa) doublet and 64-kDa antigens, respectively. By using an enzyme-linked immunosorbent assay, we observed a significantly high immunoglobulin G antibody titer to the purified M. tuberculosis 29/33-kDa doublet and 64-kDa antigens in lepromatous and tuberculoid leprosy patients, respectively, as compared with normal subjects and tuberculosis patients. This enzyme-linked immunosorbent assay serology may be useful for distinguishing two polar types of leprosy and for diagnosing leprosy in general.
authors.affiliation: Department of Dermatology, University of Amsterdam, The Netherlands.
authors.forename: P K
authors.initials: PK
authors.lastname: Das
authors.forename: A
authors.initials: A
authors.lastname: Rambukkana
authors.forename: J G
authors.initials: JG
authors.lastname: Baas
authors.forename: D G
authors.initials: DG
authors.lastname: Groothuis
authors.forename: M
authors.initials: M
authors.lastname: Halperin
mesh_terms: D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007918:Leprosy / Q000175:diagnosis / Q000276:immunology*; D015440:Leprosy, Lepromatous / Q000175:diagnosis / Q000276:immunology; D015441:Leprosy, Tuberculoid / Q000175:diagnosis / Q000276:immunology; D008970:Molecular Weight; D009169:Mycobacterium tuberculosis / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G