Biospecific adsorbents on the basis of chloroformate-activated bead cellulose.
Carbohydrate-derived polymers are activated by the chloroformate N-chlorocarbonyloxy-5-norbornene-2.3-dicarboximide (ClCOONB). The advantages of this activation method are presented. The application of bead cellulose as adsorbent for biomedical and biotechnological purposes is demonstrated. Examples for immunoglobulin purification, streptavidin isolation, and biotransformation of porcine insulin are given.
Rupprich
C
C
Academy of Sciences of the GDR, Department of Chemistry, Berlin.
Becker
M
M
Büttner
W
W
Boeden
H F
HF
Schröder
K L
KL
Hänsicke
A
A
Könnecke
A
A
D001426:Bacterial Proteins / Q000302:isolation & purification; D001710:Biotin / Q000031:analogs & derivatives; D002482:Cellulose; D002846:Chromatography, Affinity / Q000379:methods*; D007074:Immunoglobulin G / Q000302:isolation & purification; D007164:Immunosorbents; D007328:Insulin; D009636:Norbornanes; D019809:Streptavidin; D013499:Surface Properties; D014357:Trypsin
D001426:Bacterial Proteins; D007074:Immunoglobulin G; D007164:Immunosorbents; D007328:Insulin; D009636:Norbornanes; D001710:Biotin; D002482:Cellulose; D019809:Streptavidin; C069687:N-chlorocarbonyloxy-5-norborene-2,3,-dicarboximide; D014357:Trypsin; C028072:2-iminobiotin