Differential assay and biological significance of poly(ADP-ribose) polymerase activity in isolated liver nuclei.
Poly(ADP-ribosyl)ation of nuclear proteins is catalyzed by poly(ADP-ribose) polymerase. This enzyme is involved in the regulation of basic cellular functions of DNA metabolism. DNA breaks induced by DNA-damaging agents trigger the activation of poly(ADP-ribose) polymerase increasing its endogenous level. This increase modifies the pattern of poly(ADP-ribosyl)ated chromatin proteins. In this paper we describe a procedure for the isolation of intact nuclei from rat liver to be used for the endogenous activity assay. Artifactual activation of the enzyme was avoided since a very low level of DNA-strand breaks occurs during the isolation of nuclei. We present a series of experiments which prove the ability of this procedure to detect increases in endogenous liver activity without modification of the total level. The application of this technique can be useful for a better understanding of the role of early changes in poly(ADP-ribose) polymerase level in physiological conditions and during exposure to DNA-damaging agents.
Cesarone
C F
CF
Institute of General Physiology, Faculty of Sciences, University of Genoa, Italy.
Scarabelli
L
L
Giannoni
P
P
Orunesu
M
M
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002467:Cell Nucleus / Q000201:enzymology; D004247:DNA / Q000096:biosynthesis; D004249:DNA Damage; D003850:Deoxyribonuclease I / Q000494:pharmacology; D008099:Liver / Q000201:enzymology; D008297:Male; D009243:NAD / Q000378:metabolism; D011065:Poly(ADP-ribose) Polymerases / Q000032:analysis* / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D013010:Sonication / Q000009:adverse effects; D013095:Spermidine / Q000494:pharmacology
D009243:NAD; D004247:DNA; D011065:Poly(ADP-ribose) Polymerases; D003850:Deoxyribonuclease I; D013095:Spermidine