Structure and mutation of a gene encoding a Mr 33,000 phycocyanin-associated linker polypeptide.
The gene encoding a phycocyanin-associated linker polypeptide of Mr 33,000 from the cyanobacterium Synechococcus sp. PCC 7002 was found to be located adjacent and 3' to the genes encoding the alpha and beta subunits of phycocyanin. The identity of this gene, designated cpcC, was proven by matching the amino-terminal sequence of the authentic polypeptide with that predicted by the nucleotide sequence. A cpcC mutant strain of this cyanobacterium was constructed. The effect of the mutation was to prevent assembly of half the total phycocyanin into phycobilisomes. By electron microscopy, phycobilisomes from this mutant were shown to contain rod substructures composed of a single disc of hexameric phycocyanin, as opposed to two discs in the wild type. It was concluded that the Mr 33,000 linker polypeptide is required for attachment of the core-distal phycocyanin hexamer to the core-proximal one. Using absorption spectra of the wild type, CpcC-, and phycocyanin-less phycobilisomes, the in situ absorbances expected for specific phycocyanin-linker complexes were calculated. These data confirm earlier findings on isolated complexes regarding the influence of linkers on the spectroscopic properties of phycocyanin.
de Lorimier
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Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
Guglielmi
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Bryant
D A
DA
Stevens
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SE
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D000458:Cyanobacteria / Q000235:genetics* / Q000648:ultrastructure; D004247:DNA / Q000235:genetics; D045342:Light-Harvesting Protein Complexes; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009154:Mutation; D010446:Peptide Fragments / Q000235:genetics*; D045524:Phycobilisomes; D010798:Phycocyanin / Q000235:genetics*; D010860:Pigments, Biological / Q000235:genetics*; D010940:Plant Proteins / Q000032:analysis / Q000648:ultrastructure; D010957:Plasmids; D015183:Restriction Mapping
D045342:Light-Harvesting Protein Complexes; D010446:Peptide Fragments; D045524:Phycobilisomes; D010860:Pigments, Biological; D010940:Plant Proteins; D010798:Phycocyanin; D004247:DNA