In-vivo biosynthesis of acid glycosaminoglycans with radiosulfate and [3H]glucosamine in single joints from rats of different age.
Administration intraperitoneally of 35SO4 plus D-[6-3H]glucosamine of similarly specific radioactivity to rats of different ages showed uptake rates and incorporation to be higher with 35SO4 in articular cartilage. Comparing the joints studied: knee joints (femur and tibia parts), humerus, and femur heads, cartilage cells appeared to be most metabolically active (age-dependently) in femur heads. All cells, in addition to glycoproteins, synthesized in vivo isomeric chondroitin sulfates and hyaluronate at different rates.
Kleine
T O
TO
Med. Zentrum für Nervenheilkunde, Funktionsbereich Neurochemie, Universität Marburg.
Baumann
H J
HJ
D000375:Aging / Q000378:metabolism*; D000818:Animals; D002358:Cartilage, Articular / Q000378:metabolism*; D002809:Chondroitin Sulfates / Q000096:biosynthesis; D005944:Glucosamine / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006820:Hyaluronic Acid / Q000096:biosynthesis; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D006023:Glycoproteins; D006025:Glycosaminoglycans; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates; D005944:Glucosamine