Diisopropylfluorophosphate binding proteins (serine hydrolases) from normal and leukemic hematopoietic cells.
Normal and leukemic hematopoietic cell lysates were labeled with [3H]-diisopropylfluorosorophosphate ([3H]-DFP), an active site inhibitor of serine hydrolases. The labeled proteins in the lysates were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by counting of gel segments for radioactivity. The results indicate the presence of distinct [3H]-DFP binding patterns for different normal and leukemic hematopoietic cells; significantly lower labeling in normal or leukemic lymphoid cells compared to myeloid or monocytoid cells; lower labeling in acute myeloblastic leukemia (FAB-M1) as compared to acute myelomonocytic leukemia (FAB-M4), chronic myelomonocytic leukemia or monocytes and an increase in [3H]-DFP binding with cell maturation along granulocytic series. Thus, these patterns could be useful in discriminating acute lymphoblastic leukemia from myeloid/monocytoid types of leukemia and for following maturation of myeloid cells, and perhaps for studying functional or maturation defects in hematopoietic cells in other pathological conditions.
Gonzales
C R
CR
Department of Laboratory Medicine, Roswell Park Memorial Institute, Buffalo, N.Y.
Sahai Srivastava
B I
BI
Fitzpatrick
J E
JE
D001782:Blood Donors; D003937:Diagnosis, Differential; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D007531:Isoflurophate / Q000097:blood; D007938:Leukemia / Q000097:blood* / Q000175:diagnosis; D011485:Protein Binding; D012588:Scintillation Counting; D012697:Serine Endopeptidases / Q000097:blood*; D013057:Spectrum Analysis; D014316:Tritium
D014316:Tritium; D007531:Isoflurophate; D012697:Serine Endopeptidases