[Localization in Escherichia coli of transcribed regions of the broad host range plasmid pBS222].
The set of insertions of the CmR-gene region devoid of the promoter into the broad host-range plasmid pBS222 regions transcribed in Escherichia coli cells has been obtained and characterized. Three transcribed regions of the plasmid that are dispensable for life functions of the plasmid have been identified by the insertions technique. The deleted plasmid derivatives have been isolated suitable for using as the broad host-range vectors.
Dubeĭkovskiĭ
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AN
Boronin
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AM
D002702:Chloramphenicol Resistance; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000235:genetics; D015183:Restriction Mapping; D014158:Transcription, Genetic
Lokalizatsiia transkribiruemykh v Escherichia coli oblasteĭ plazmidy pBS222 s shirokim krugom khoziaev.