[Effects of ethanol on pepsinogen release from isolated guinea pig gastric chief cells].
The effects of ethanol on pepsinogen release from isolated guinea pig gastric chief cells were investigated. Ethanol, at concentrations of 300 mM to 900 mM, dose-dependently stimulated increases in pepsinogen release, initial Ca influx rate and intracellular free Ca concentration ([Ca2+]i) without affecting chief cells' viability. The increases in all parameters were inhibited by La3+ or EGTA, but not by nifedipine or verapamil. COOH-terminal octapeptide of cholecystokinin (CCK8) also stimulated increases in pepsinogen release, initial Ca influx rate and [Ca2+]i. However, the increases were dependent on not only extracellular Ca2+ but also intracellular Ca2+ mobilization. These results suggest that ethanol stimulates Ca2+ influx via the mechanism different from that of CCK8 and thereby stimulates pepsinogen release from isolated guinea pig gastric chief cells.
Konda
Y
Y
Sakamoto
C
C
Nishisaki
H
H
Nakano
O
O
Matsuda
K
K
Wada
K
K
Nagao
M
M
Matozaki
T
T
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004533:Egtazic Acid / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006168:Guinea Pigs; D010435:Pepsinogens / Q000378:metabolism*; D013268:Stimulation, Chemical
D010435:Pepsinogens; D000431:Ethanol; D004533:Egtazic Acid; D002118:Calcium