Immobilization of callus tissue cells of Salvia miltiorrhiza and the characteristics of their products.
The Salvia miltiorrhiza callus tissue cells were entrapped with 3% alginate. The immobilized cells were incubated in medium with LS + KT 0.1 + NAA 1.0 containing 3% sucrose at 25 degrees C for nearly one month. After incubation, the medium free from cells was extracted with ether 3 times. After evaporation, the residue of the ether extract was employed to determine the content on TLC and HPLC. The results showed that the incubation system mentioned above could continuously secrete the main components of S. miltiorrhiza, tanshinone IIA and cryptotanshinone, which were almost the same as the extract of S. miltiorrhiza roots. In addition, suspension incubation of callus tissue cells, the conditions of immobilization with alginate, the stability of immobilized cells and the characteristics of products were also examined.
Yuan
J M
JM
Institute of Molecular Science, Shanxi University, Taiyuan.
Tao
L L
LL
Xu
J T
JT
D045784:Abietanes; D000464:Alginates / Q000378:metabolism; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003470:Culture Media; D004365:Drugs, Chinese Herbal; D007700:Kinetics; D010616:Phenanthrenes
D045784:Abietanes; D000464:Alginates; D003470:Culture Media; D004365:Drugs, Chinese Herbal; D010616:Phenanthrenes; C021751:tanshinone; C037886:cryptotanshinone