Lipopolysaccharide-unresponsive mutant pre-B-cell lines blocked in NF-kappa B activation.
NF-kappa B activation is a crucial late step in the induction of immunoglobulin kappa light-chain gene expression in pre-B cells by lipopolysaccharide (LPS). We have analyzed NF-kappa B activation in three independent mutant lines of 70Z/3 pre-B cells which are unresponsive to LPS. All three variant cell lines failed to activate NF-kappa B when induced with LPS or the phorbol ester 12-O-tetradecanoylphorbol 13-acetate. However, all three cell lines contained functional NF-kappa B, as revealed by detergent treatment of cytoplasmic extracts. Moreover, cycloheximide induced limited activation of NF-kappa B comparable to that in wild-type 70Z/3 pre-B cells in two of the three variant lines. These results indicate that the mutations blocking kappa gene induction in these variant 70Z/3 pre-B-cell lines affect NF-kappa B activation.
Briskin
M
M
Molecular Biology Institute, University of California School of Medicine, Los Angeles 90024.
Damore
M
M
Law
R
R
Lee
G
G
Kincade
P W
PW
Sibley
C H
CH
Kuehl
M
M
Wall
R
R
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008213:Lymphocyte Activation; D051379:Mice; D009154:Mutation; D016328:NF-kappa B; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014157:Transcription Factors / Q000502:physiology*; D015533:Transcriptional Activation
D007145:Immunoglobulin kappa-Chains; D008070:Lipopolysaccharides; D016328:NF-kappa B; D014157:Transcription Factors; D013755:Tetradecanoylphorbol Acetate