title: Chemical enhancement of cisplatin cytotoxicity in a human ovarian and cervical cancer cell line.
abstract: While many advances have been made in the chemotherapy of gynecologic cancers, treatment failures remain a major clinical problem. A growing understanding of the mechanisms of tumor cell resistance to antineoplastic drugs provides a framework for the development of chemotherapy regimens containing agents capable of modulating tumor response. Using a short-term ATP bioluminescence assay we studied the ability of two methylxanthines (caffeine, pentoxifylline) and an inhibitor of ADP-ribosyl transferase (3-aminobenzamide) to enhance cisplatin cytotoxicity in gynecologic cancer cell lines. Our findings of significantly enhanced cisplatin-induced cytotoxicity with two different analysis techniques confirms the effectiveness of these agents. These results may have future clinical significance.
authors.affiliation: Division of Gynecologic Oncology, University of Miami, Florida 33101.
authors.forename: G M
authors.initials: GM
authors.lastname: Boike
authors.forename: E
authors.initials: E
authors.lastname: Petru
authors.forename: B U
authors.initials: BU
authors.lastname: Sevin
authors.forename: H E
authors.initials: HE
authors.lastname: Averette
authors.forename: T C
authors.initials: TC
authors.lastname: Chou
authors.forename: M
authors.initials: M
authors.lastname: Penalver
authors.forename: D
authors.initials: D
authors.lastname: Donato
authors.forename: M
authors.initials: M
authors.lastname: Schiano
authors.forename: S G
authors.initials: SG
authors.lastname: Hilsenbeck
authors.forename: J
authors.initials: J
authors.lastname: Perras
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D001549:Benzamides; D002110:Caffeine / Q000494:pharmacology; D002478:Cells, Cultured; D002945:Cisplatin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D004357:Drug Synergism; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D010051:Ovarian Neoplasms / Q000188:drug therapy*; D010431:Pentoxifylline / Q000494:pharmacology; D011065:Poly(ADP-ribose) Polymerases / Q000494:pharmacology; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy*
chemical_list: D001549:Benzamides; D002110:Caffeine; C025160:3-aminobenzamide; D000255:Adenosine Triphosphate; D011065:Poly(ADP-ribose) Polymerases; D002945:Cisplatin; D010431:Pentoxifylline