Lack of permissivity of human monoclonal CD4+ lymphocytes to HIV.
Although knowledge has accumulated about GP110-CD4 interaction, viral penetration into human CD4+ lymphocytes remains unclear, in spite of the fact that all studies on HIV infection were performed on cell-transformed lineages, or on human polyclonal CD4+ cells. In order to investigate this viral entrance into susceptible cells, we studied the permissivity of 13 human monoclonal CD4+ lymphocytes by means of reverse transcriptase (RT) assay and immunocapture. We demonstrated a differential susceptibility to HIV of these CD4+ clones. In a second experiment, HIV infection was studied: (1) sequentially by RT assay and P24 immunocapture on several clones; (2) by cocultivation of infected clones with umbilical cord lymphocytes. These experiments suggested existence of permissive and "nonpermissive" CD4+ monoclonal lymphocytes. Slot blot, then PCR, revealed that proviral DNA sequences were detectable in all clones, but were present at lower levels in nonpermissive clones.
Chapel
A
A
Laboratoire de Neuropathologie Expérimentale et de Neurovirologie, CRSSA CEA DPS/SPE, Paris, France.
Bourges
J F
JF
Bensussan
A
A
d'Auriol
L
L
Vilmer
E
E
Dormont
D
D
D000911:Antibodies, Monoclonal / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology*; D002999:Clone Cells / Q000276:immunology; D004279:DNA, Viral / Q000502:physiology; D005312:Fetal Blood / Q000166:cytology; D015658:HIV Infections / Q000473:pathology; D006679:HIV Seropositivity; D015497:HIV-1 / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007371:Interferon-gamma / Q000096:biosynthesis; D016133:Polymerase Chain Reaction; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000382:microbiology; D013601:T-Lymphocytes / Q000276:immunology / Q000502:physiology*; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D004279:DNA, Viral; D007371:Interferon-gamma