Mapping of mouse carbonic anhydrase-3, Car-3: another locus in the homologous region of mouse chromosome 3 and human chromosome 8.
At least six separate genes determining tissue- and organelle-specific isoforms of carbonic anhydrase are known. We have determined the chromosome location of one of these genes, carbonic anhydrase-3 (Car-3), in the mouse and carried out a linkage analysis of Car-1, Car-2, and Car-3. Car-3 has been assigned to band 3A2 by in situ hybridization. We identified a PstI restriction fragment length polymorphism between Mus spretus and Mus mus domesticus and, by using an interspecific backcross, showed that Car-3 is 2.4 +/- 1.7% SE from both Car-1 and Car-2, calculating genetic distance as percentage recombination. No recombinants were found between Car-1 and Car-2 in 100 backcross offspring, and when these data are combined with earlier results, these two loci are estimated to be 1.2 cM from each other at the 95% confidence interval. The three homologous carbonic anhydrase loci in man had earlier been assigned to 8q22, and the finding of linkage of Car-3 to Car-1 and Car-2 in the mouse adds another locus to the conserved segments on mouse chromosome 3 and human chromosome 8.
Beechey
C
C
MRC Radiobiology Unit, Chilton, Didcot, Oxon, United Kingdom.
Tweedie
S
S
Spurr
N
N
Ball
S
S
Peters
J
J
Edwards
Y
Y
D000818:Animals; D002256:Carbonic Anhydrases / Q000235:genetics*; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D003433:Crosses, Genetic; D005260:Female; D005796:Genes; D005819:Genetic Markers; D006801:Humans; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics; D009115:Muridae / Q000235:genetics*; D010802:Phylogeny; D011995:Recombination, Genetic; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D005819:Genetic Markers; D002256:Carbonic Anhydrases