The sequence of the hemoregulatory peptide is present in Gi alpha proteins.
The hemoregulatory peptide PyroGlu-Glu-Asp-Cys-Lys (HP5b), which inhibits myelopoietic colony formation in vitro, is shown to be a sequence motif which is also part of the effector domain of Gi alpha proteins. Out of 8 synthetic peptides with sequence variations of HP5b, those with the closest similarity to the Gi alpha sequence are biologically active. The inhibitory effect appears to be dependent on the blocked N-terminus. It is postulated that these peptides may interfere with signal transduction mediated by Gi alpha proteins.
Laerum
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Gade Institute, Department of Pathology, University of Bergen, Haukeland Hospital, Norway.
Frostad
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Tøn
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Kamp
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D000595:Amino Acid Sequence; D000818:Animals; D019204:GTP-Binding Proteins / Q000502:physiology*; D006410:Hematopoiesis / Q000187:drug effects*; D066298:In Vitro Techniques; D051379:Mice; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000502:physiology*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D013329:Structure-Activity Relationship
D009842:Oligopeptides; C040758:hemoregulatory peptide 5b; D019204:GTP-Binding Proteins; D011761:Pyrrolidonecarboxylic Acid