Isolation of a second cryptic plasmid from Mycoplasma mycoides subsp. mycoides.
Plasmids have rarely been detected in organisms constituting the genus Mycoplasma. Recently, the isolation of a cryptic plasmid from Mycoplasma mycoides subsp. mycoides has been described, and we report here the isolation of a second cryptic plasmid from this species. Restriction map and Southern blot analyses show that the second plasmid is distinct from the previously described plasmid, although a limited region of homology was detected. The availability of mycoplasmal cryptic plasmids may lead to the development of cloning vectors that replicate in these organisms.
Dybvig
K
K
Department of Comparative Medicine, University of Alabama, Birmingham 35294.
Khaled
M
M
D005822:Genetic Vectors; D009176:Mycoplasma mycoides / Q000235:genetics*; D010957:Plasmids; D015183:Restriction Mapping