A transferable plasmid associated with AS-48 production in Enterococcus faecalis.
Enterococcus faecalis S-48 produces a peptide antibiotic, AS-48, and a bacteriocin, Bc-48. We have isolated mutants that lack these inhibitory characteristics. Further analysis of the mutants indicates that a plasmid of 56 kilobases (pMB2) may harbor the genes for AS-48. In conjugation experiments, pMB2 has been transferred into a plasmid-free OG1X strain of E. faecalis. The OG1X(pMB2) transconjugant produces the antibiotic AS-48 in solid medium, and the MIC of AS-48 for this strain is the same as that of the donor strain.
Martínez-Bueno
M
M
Departamento de Microbiología, Facultad de Ciencias, Universidad de Granada, Spain.
Gálvez
A
A
Valdivia
E
E
Maqueda
M
M
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D013293:Enterococcus faecalis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D009154:Mutation; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D010957:Plasmids
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010455:Peptides; C051034:BacA protein, Enterococcus faecalis