title: Occurrence of D-rhamnan as the common antigen reactive against monoclonal antibody E87 in Pseudomonas aeruginosa IFO 3080 and other strains.
abstract: S. Sawada and co-workers reported that a monoclonal antibody (MAb), E87, interacted with about 80% of Pseudomonas aeruginosa isolates, and they separated a rhamnose-rich polysaccharide as the probable antigen for MAb E87 from P. aeruginosa IFO 3080 (S. Sawada, T. Kawamura, Y. Masuho, and K. Tomibe, J. Infec. Dis. 152:1290-1299, 1985). In the present study, the rhamnose-rich polysaccharide was shown to be structurally and immunologically identical to the D-rhamnan of P. aeruginosa IID 1008 (S. Yokota, S. Kaya, S. Sawada, T. Kawamura, Y. Araki, and E. Ito, Eur. J. Biochem. 167:203-209, 1987). Furthermore, a set of enzymes responsible for the formation of GDP-rhamnose (probably in a D-form) from GDP-D-mannose was found in the 100,000 x g supernatant fractions obtained from all of nine P. aeruginosa strains reactive against MAb E87. The result strongly supports a possibility that lipopolysaccharides having a D-rhamnan chain widely occur as the common antigen among various P. aeruginosa isolates.
authors.affiliation: Department of Chemistry, Faculty of Science, Hokkaido University, Sapporo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yokota
authors.forename: S
authors.initials: S
authors.lastname: Kaya
authors.forename: Y
authors.initials: Y
authors.lastname: Araki
authors.forename: E
authors.initials: E
authors.lastname: Ito
authors.forename: T
authors.initials: T
authors.lastname: Kawamura
authors.forename: S
authors.initials: S
authors.lastname: Sawada
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000032:analysis*; D003837:Deoxy Sugars / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000032:analysis*; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000276:immunology; D011550:Pseudomonas aeruginosa / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D003837:Deoxy Sugars; D008351:Mannans; D011135:Polysaccharides, Bacterial; C065836:rhamnan