Inhibition of basic calcium phosphate crystal-induced mitogenesis by phosphocitrate.
Basic calcium phosphate crystals control the traverse of cells from the Go/G1 to S-phase of the cell cycle and initiate proliferation by rendering fibroblasts competent to respond to insulin-like growth factors in plasma. Simultaneous addition of phosphocitrate [a powerful inhibitor of hydroxyapatite crystallization] to cells exposed to basic calcium phosphate crystals caused a dose-dependent inhibition of crystal-induced DNA synthesis and c-fos transcription. This inhibition was specific for crystal-induced mitogenesis, since similar concentrations of phosphocitrate had no effects on either PDGF or 10% calf serum-induced thymidine incorporation and c-fos transcription.
Cheung
H S
HS
Dept. of Medicine, Medical College of Wisconsin, Milwaukee 53226.
Sallis
J D
JD
Mitchell
P G
PG
Struve
J A
JA
D000818:Animals; D015152:Blotting, Northern; D002130:Calcium Phosphates / Q000494:pharmacology*; D002460:Cell Line; D002951:Citrates / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D015870:Gene Expression / Q000187:drug effects; D051379:Mice; D008938:Mitosis / Q000187:drug effects*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011518:Proto-Oncogene Proteins / Q000235:genetics; D016760:Proto-Oncogene Proteins c-fos; D014158:Transcription, Genetic / Q000187:drug effects
D002130:Calcium Phosphates; D002951:Citrates; D010982:Platelet-Derived Growth Factor; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C014148:phosphocitrate; C485838:calcium phosphate, monobasic, anhydrous; D004247:DNA; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous