Growth of Bacillus stearothermophilus on glycerol in chemostat culture: expression of an unusual phenotype.
Bacillus stearothermophilus grew readily on glycerol in carbon-limited chemostat culture and expressed a high carbon conversion efficiency. However, the strain of organism used (probably B. stearothermophilus var. nondiastaticus) proved particularly sensitive to glycerol, both respiration and growth being severely impeded by any surfeit of this compound. Sensitivity was found to correlate with an exceptionally high level of expression of glycerol kinase [activities of more than 80 mumol min-1 (mg protein)-1 were manifest in crude cell-free extracts], coupled with low activities of methylglyoxal synthase and of glyoxylase (enzymes of the methylglyoxal bypass). It is proposed that metabolic dysfunction results from an uncontrolled gross accumulation of glycerol phosphate (and early products of its metabolism) within the cells, coupled with depletion of the intracellular phosphate pool.
Burke
R M
RM
Department of Molecular Biology and Biotechnology, University of Sheffield, UK.
Tempest
D W
DW
D002245:Carbon Dioxide / Q000378:metabolism; D019757:Carbon-Oxygen Lyases; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D001411:Geobacillus stearothermophilus / Q000201:enzymology / Q000254:growth & development*; D005990:Glycerol / Q000378:metabolism*; D005991:Glycerol Kinase / Q000235:genetics* / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008190:Lyases / Q000096:biosynthesis* / Q000378:metabolism; D010101:Oxygen Consumption; D010641:Phenotype; D013696:Temperature
D003470:Culture Media; D005994:Glycerophosphates; D002245:Carbon Dioxide; D005991:Glycerol Kinase; D008190:Lyases; D019757:Carbon-Oxygen Lyases; C021578:methylglyoxal synthase; D005990:Glycerol