Histochemical demonstration of non-specific esterases and non-specific acid phosphatases using menadiol substrates.
Although many synthetic substrates and methods are available for the histochemical detection of non-specific esterases and non-specific acid phosphatase, there are still further possibilities to investigate these hydrolases histochemically. This was shown for menadiol diacetate and menadiol diphosphate using tetrazolium salt, simultaneous azo-dye as well as metal salt methods in many rat tissues. In comparison, the azo-dye procedure with various Fast salts or hexazonium Pararosaniline or New Fuchsin delivered less satisfactory results; precisely localized stain in sufficient amounts was obtained for non-specific esterases using nitro BT, tetranitro BT or benzothiazolystyrylphthalhydrazidyl tetrazolium (BSPT) and for non-specific acid phosphatase with BSPT in the tetrazolium salt method or using cerium ions for phosphate trapping in the diaminobenzidine-nickel-hydrogen peroxide procedure.
Dikow
A
A
Department of Cell Biology and Morphology, Bulgarian Academy of Sciences, Sofia.
Gossrau
R
R
D000135:Acid Phosphatase / Q000032:analysis*; D000818:Animals; D001391:Azo Compounds; D004950:Esterases / Q000032:analysis*; D005260:Female; D006651:Histocytochemistry; D008297:Male; D008670:Metals; D051381:Rats; D011919:Rats, Inbred Strains; D013778:Tetrazolium Salts; D014812:Vitamin K / Q000031:analogs & derivatives* / Q000378:metabolism
D001391:Azo Compounds; D008670:Metals; D013778:Tetrazolium Salts; D014812:Vitamin K; D004950:Esterases; D000135:Acid Phosphatase; C023885:menadiol