Enhancement of experimental metastatic ability by tumor necrosis factor-alpha alone or in combination with interferon-gamma.
The effects of recombinant tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) on B16 mouse melanoma experimental metastatic ability and major histocompatibility complex (H-2b) antigens expression were studied. B16 cells exposed in vitro to TNF-alpha had an increased H-2 expression and were more metastatic than untreated cells. The simultaneous treatment with TNF-alpha and IFN-gamma amplified the enhancement of experimental metastasis and all other effects obtained with TNF-alpha alone. The B16 clone B78H1, selectively resistant to H-2 induction and to enhancement of metastatic ability by IFN-gamma, was not affected by treatment with TNF-alpha and with TNF-alpha + IFN-gamma. These findings contribute to a better understanding of the pleiotropic effects of TNF, some of which can have opposing actions in the complex tumor-host relationships.
Lollini
P L
PL
Istituto di Cancerologia, Università di Bologna, Italy.
De Giovanni
C
C
Nicoletti
G
G
Bontadini
A
A
Tazzari
P L
PL
Landuzzi
L
L
Scotlandi
K
K
Nanni
P
P
D000818:Animals; D004305:Dose-Response Relationship, Drug; D006183:H-2 Antigens / Q000032:analysis; D007371:Interferon-gamma / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000187:drug effects; D008297:Male; D008546:Melanoma, Experimental / Q000276:immunology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D006183:H-2 Antigens; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma