Highly sensitive determination of PLP in human plasma with HPLC method.
To investigate the validity of using HPLC procedure with the fluorescence detection for the estimation of the pyridoxal 5'-phosphate (PLP) concentration in human blood plasma, we modified the conditions of our previous HPLC method for PLP. By changing the pH of the mobile phase (pH 5.5) and by altering the detection wavelengths in fluorescence (excitation at 322 nm and emission at 417 nm), both the improved sensitivity for pyridoxic acid 5'-phosphate and elimination of interfering extraneous peaks were attained. We reported the total vitamin B6 content of healthy adult volunteers obtained by this HPLC method.
Hirose
N
N
Institute for Food Science, Faculty of Agriculture, Gifu University, Japan.
Kubo
N
N
Tsuge
H
H
D000293:Adolescent; D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D005453:Fluorescence; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008875:Middle Aged; D011732:Pyridoxal Phosphate / Q000097:blood*; D011736:Pyridoxine / Q000097:blood; D012680:Sensitivity and Specificity
D011732:Pyridoxal Phosphate; D011736:Pyridoxine