Use of the tyrosinase gene from Streptomyces to probe promoter sequences for Escherichia coli.
We have constructed a promoter-probe vector utilizing the expression of a promoter-less tyrosinase derived from Streptomyces plasmid pIJ702. The vector, pMX100, has single sites for EcoRI, KpnI, BamHI, XbaI, SalI, and SphI for cloning promoter sequences. When the tac promoter was inserted into pMX100, E. coli harboring the chimeric plasmid produced the melanin pigment.
Sugiyama
M
M
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Japan.
Nomura
H
H
Nimi
O
O
D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D014442:Monophenol Monooxygenase / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*
D014442:Monophenol Monooxygenase