title: Constraction of a subtracted cDNA library using oligo(dT)-latex.
abstract: An efficient method for constraction of subtracted cDNA library was developed using oligo(dT 30. Latex and PCR. This method improved the chances for identifying cDNA clones corresponding to scarce class of mRNA that is expressed differentially during cellular growth and differentiation.
authors.affiliation: Department of Applied Biological Science, Science University of Tokyo, Chiba, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Hara
authors.forename: T
authors.initials: T
authors.lastname: Kato
authors.forename: S
authors.initials: S
authors.lastname: Nakada
authors.forename: T
authors.initials: T
authors.lastname: Nakajima
authors.forename: H
authors.initials: H
authors.lastname: Tsurui
authors.forename: K
authors.initials: K
authors.lastname: Oda
mesh_terms: D000081:Acetamides; D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA; D015723:Gene Library; D006801:Humans; D007840:Latex; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
chemical_list: D000081:Acetamides; D007840:Latex; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; C027903:oligo (dT); D004247:DNA; C014026:hexamethylene bisacetamide