title: [Inhibition of glycogen synthase I from rabbit skeletal muscles by 1,5-gluconolactone].
abstract: The effect of 1.5-gluconolactone on the activity of rabbit skeletal muscle glycogen synthase I was investigated. Using statistic methods (pair regressive analysis) and computer analysis on a Robotron EC 1834 personal computer, it was found that 1.5-gluconolactone is a true competitive inhibitor of the enzyme in respect of UDP-glucose. Similar to UDP, 1.5-gluconolactone increases the Km value for UDP-glucose without affecting the V value. The Ki value for 1.5-gluconolactone is equal to 123 + 8 microM and it coincides with the Km value for UDP-glucose.
authors.forename: S Iu
authors.initials: SIu
authors.lastname: Sundukov
authors.forename: G A
authors.initials: GA
authors.lastname: Solov'eva
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D005942:Gluconates / Q000494:pharmacology*; D006006:Glycogen Synthase / Q000037:antagonists & inhibitors*; D066298:In Vitro Techniques; D007700:Kinetics; D007783:Lactones; D009132:Muscles / Q000201:enzymology*; D011817:Rabbits; D014532:Uridine Diphosphate Glucose / Q000378:metabolism
chemical_list: D005942:Gluconates; D007783:Lactones; D006006:Glycogen Synthase; D014532:Uridine Diphosphate Glucose; C010730:beta-glucono-1,5-lactone
vernacular_title: Ingibirovanie glikogensintazy I skeletnykh myshts krolika 1,5-gliukonolaktonom.