Protein kinase C modulation of queuine uptake in cultured human fibroblasts.
Protein kinase C modulates the activity of a highly specific uptake mechanism for queuine in cultured human fibroblasts. Activators of protein kinase C induce an increased uptake rate for the radiolabeled analog of queuine, rQT3. The protein kinase C inhibitors, H-7, staurosporine and sphingosine all induced a dramatic decrease in the uptake rate of rQT3. This suggests that protein kinase C is tied to efficient cellular uptake of queuine. Uptake is prerequisite to the modification of transfer RNA with queuine. Perturbation of queuine-modified transfer RNA levels has been associated with neoplastic transformation, differentiation and growth control.
Elliott
M S
MS
Old Dominion University, Department of Chemistry and Biochemistry, Norfolk, Virginia 23529.
Crane
D L
DL
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000470:Alkaloids / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D004075:Diglycerides / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005347:Fibroblasts / Q000378:metabolism; D006133:Growth Substances / Q000494:pharmacology; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007546:Isoquinolines / Q000494:pharmacology; D008297:Male; D010703:Phorbol Esters / Q000494:pharmacology; D010718:Phosphatidylserines / Q000494:pharmacology; D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000502:physiology*; D013110:Sphingosine / Q000494:pharmacology; D019311:Staurosporine
D000470:Alkaloids; D004075:Diglycerides; D006133:Growth Substances; D007546:Isoquinolines; D010703:Phorbol Esters; D010718:Phosphatidylserines; D010879:Piperazines; C008876:phorbol-12,13-didecanoate; D000001:Calcimycin; D006147:Guanine; C019549:queuine; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011493:Protein Kinase C; D019311:Staurosporine; D013110:Sphingosine; C013965:diolein