title: Processing and intracellular transport of rubella virus structural proteins in COS cells.
abstract: Plasmids encoding rubella virus (RV) structural proteins C-E2-E1, E2-E1, E2, and E1 have been constructed in the eukaryotic expression vector pCMV5. The processing and intracellular transport of these proteins have been examined by transient expression of the cDNAs in COS cells. Compared to alphaviruses, processing of RV glycoprotein moieties occurred relatively slowly and the transport of glycoproteins E2 and E1 to the plasma membrane was inefficient. Indirect immunofluorescence revealed that the majority of RV antigen in transfected and infected COS cells was localized to the Golgi region, including the capsid protein. Accumulation of capsid protein in the juxtanuclear region was determined to be RV glycoprotein dependent. Unlike alphaviruses, RV E1 did not require E2 for targeting to the Golgi where it was retained. E2 was however necessary for cell surface expression of E1. This study revealed that the processing and transport of RV structural proteins is quite different from alphaviruses and that the accumulation of antigens in the Golgi region may be significant in light of previous reports which suggest that RV buds from the internal membranes in some cell types.
authors.affiliation: Department of Pathology, Faculty of Medicine, University of British Columbia Research Center, Vancouver, Canada.
authors.forename: T C
authors.initials: TC
authors.lastname: Hobman
authors.forename: M L
authors.initials: ML
authors.lastname: Lundstrom
authors.forename: S
authors.initials: S
authors.lastname: Gillam
mesh_terms: D000215:Acylation; D000818:Animals; D000956:Antigens, Viral / Q000378:metabolism*; D001692:Biological Transport; D015153:Blotting, Western; D002478:Cells, Cultured; D004279:DNA, Viral / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000276:immunology*; D006056:Golgi Apparatus / Q000276:immunology; D000882:Haplorhini; D011233:Precipitin Tests; D012412:Rubella virus / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014759:Viral Envelope Proteins; D015678:Viral Structural Proteins / Q000378:metabolism*
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; D006023:Glycoproteins; D014759:Viral Envelope Proteins; D015678:Viral Structural Proteins; C061826:E1 envelope protein, Rubella virus