Histochemical and immunohistochemical evidence for hepatic zone 3 distribution of alcohol dehydrogenase in rats.
The distribution of alcohol dehydrogenase in the hepatic acinus was examined by both histochemical and immunohistochemical approaches. The immunohistochemical method using anti-alcohol dehydrogenase antibody indicated zone 3 predominance of this enzyme in the hepatic acinus, whereas a conventional histochemical method showed slight zone 1 predominance. However, when the histochemical technique was improved by using 2% glutaraldehyde instead of formalin for fixation and by adding phenazine methosulfate (0.33 mmol/L) to the staining incubation mixture, this method also supported zone 3 predominance of alcohol dehydrogenase. Evidence for zone 3 distribution of alcohol dehydrogenase may be of value in elucidating the mechanism of zone 3 liver damage by alcohol.
Kato
S
S
Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
Ishii
H
H
Aiso
S
S
Yamashita
S
S
Ito
D
D
Tsuchiya
M
M
D000426:Alcohol Dehydrogenase / Q000032:analysis*; D000818:Animals; D002478:Cells, Cultured; D005557:Formaldehyde; D005976:Glutaral; D006651:Histocytochemistry; D006652:Histological Techniques; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008297:Male; D008773:Methylphenazonium Methosulfate; D051381:Rats; D011919:Rats, Inbred Strains
D007202:Indicators and Reagents; D005557:Formaldehyde; D008773:Methylphenazonium Methosulfate; D000426:Alcohol Dehydrogenase; D005976:Glutaral