Variations in the composition of arterial wall isomeric chondroitin sulfate proteoglycans among different animal species.
1. Isomeric chondroitin sulfate proteoglycans were extracted from human, bovine, swine and rabbit aortas by 4 M guanidine-HCl and were fractionated and purified by CsCl isopycnic centrifugation, Sepharose CL-4B gel filtration, DEAE-Sepharose ion-exchange chromatography and octyl-Sepharose hydrophobic interaction chromatography. 2. The molecular size and the composition of isomeric chondroitin sulfate proteoglycans varied among species. Variations were also noted in the composition and molecular weight of constituent glycosaminoglycan chains. 3. Observations made on chondroitinase ABC and chondroitinase AC digests of proteoglycans indicate that dermatan sulfate is linked to the core proteins through chondroitin sulfates.
Radhakrisnamurthy
B
B
Department of Medicine, Louisiana State University School of Medicine, New Orleans 70112.
Srinivasan
S R
SR
Ruiz
H A
HA
Dalferes
E R
ER
Berenson
G S
GS
D000328:Adult; D000818:Animals; D001011:Aorta / Q000737:chemistry*; D002417:Cattle; D002809:Chondroitin Sulfates / Q000302:isolation & purification*; D002850:Chromatography, Gel; D006025:Glycosaminoglycans / Q000302:isolation & purification; D006801:Humans; D007536:Isomerism; D008875:Middle Aged; D008970:Molecular Weight; D011509:Proteoglycans / Q000302:isolation & purification*; D011817:Rabbits; D013045:Species Specificity
D006025:Glycosaminoglycans; D011509:Proteoglycans; D002809:Chondroitin Sulfates