Oxidation phenotyping with debrisoquine in Germany (East).
An hplc method is described which determines debrisoquine and its metabolite, 4-hydroxydebrisoquine, quantitatively after conversion of the guanidine moieties into the corresponding pyrimidines. Sensitivity, precision, and accuracy have been sufficient enough for the reliable hydroxylation phenotyping of 145 non-related healthy volunteers (64 males, 81 females, 31-80 years). 17 (11%) were poor metabolizers of debrisoquine (gene frequency: 34.2%).
Zschiesche
M
M
Department of Clinical Pharmacology, Ernst-Moritz-Arndt-Universität Greifswald GDR.
Hanke
W
W
Siegmund
W
W
Franke
G
G
Wilke
A
A
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002851:Chromatography, High Pressure Liquid; D003647:Debrisoquin / Q000031:analogs & derivatives / Q000032:analysis; D005260:Female; D005858:Germany; D006801:Humans; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D010641:Phenotype; D013056:Spectrophotometry, Ultraviolet
C012359:4-hydroxydebrisoquin; D003647:Debrisoquin