Fluorescence in situ hybridization on metaphase chromosomes with biotinylated probes. In situ hybridization, biotin labeling, cosmids, gene mapping, oncogene amplification.
In situ hybridization on metaphase chromosomes have been used to localize specific nucleic acid sequences. Initially, the nucleic acid probes were labeled isotopically. Over the past few years, non isotopic techniques have considerably progressed, and are being applied to a large spectrum of biological and clinical problems.
Cherif
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U301 and SDI 15954 I CNRS, Institut de Génétique Moléculaire, Paris, France.
Derré
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Berger
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R
D001710:Biotin; D002877:Chromosomes, Human / Q000648:ultrastructure*; D002880:Chromosomes, Human, Pair 11 / Q000648:ultrastructure; D002896:Chromosomes, Human, Pair 6 / Q000648:ultrastructure; D003360:Cosmids; D005784:Gene Amplification; D016259:Genes, myc; D006801:Humans; D008677:Metaphase; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured / Q000648:ultrastructure
D001710:Biotin