title: Amino acid sequence of pheromone-inducible surface protein in Enterococcus faecalis, that is encoded on the conjugative plasmid pPD1.
abstract: The major pheromone-inducible protein, PD78, believed to contribute to bacterial conjugation, was purified from Enterococcus (formerly Streptococcus) faecalis cells containing the plasmid pPD1. A cloned EcoRI-BglII 3.6-kbp fragment of the plasmid pAM351 (pPD1::Tn916) contained an open reading frame corresponding to 467 amino acid residues representing PD78. In a central region of the deduced protein, there is a repeated sequence of X-X-Pro that is repeated 15 times. This is analogous to the Gln-Gln-Pro repeat in the C-terminal region of TraD product encoded on the R100 plasmid in Escherichia coli.
authors.affiliation: Department of Agricultural Chemistry, University of Tokyo, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Nakayama
authors.forename: H
authors.initials: H
authors.lastname: Nagasawa
authors.forename: A
authors.initials: A
authors.lastname: Isogai
authors.forename: D B
authors.initials: DB
authors.lastname: Clewell
authors.forename: A
authors.initials: A
authors.lastname: Suzuki
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000032:analysis; D013293:Enterococcus faecalis / Q000187:drug effects / Q000235:genetics*; D008969:Molecular Sequence Data; D010675:Pheromones / Q000494:pharmacology*; D010957:Plasmids
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010675:Pheromones; C064923:TraF protein, Enterococcus faecalis