Identification of ester glucuronide and sulfate conjugates of 5-hydroxy-6-methoxyindole-2-carboxylic acid and 6-hydroxy-5-methoxyindole-2-carboxylic acid in melanoma urine.
HPLC analysis of urine sample from a patient with wide-spread melanoma revealed the presence of unknown indolic compounds at extraordinarily high levels, detectable with electrochemical and/or fluorescent detectors. By enzymic and chemical hydrolyses, they were identified as ester glucuronide and sulfate conjugates of 5-hydroxy-6-methoxyindole-2-carboxylic acid and 6-hydroxy-5-methoxyindole-2-carboxylic acid. Urine samples from B16 melanoma-bearing mice contained the sulfate conjugates but not the ester glucuronide conjugates.
Wakamatsu
K
K
School of Hygiene, Fujita Health University, Aichi, Japan.
Ito
S
S
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005965:Glucuronates / Q000652:urine*; D006801:Humans; D007211:Indoles / Q000652:urine*; D008545:Melanoma / Q000652:urine*; D051379:Mice; D012878:Skin Neoplasms / Q000652:urine*; D013431:Sulfates / Q000378:metabolism*
D005965:Glucuronates; D007211:Indoles; D013431:Sulfates; C017407:5-hydroxy-6-methoxy-2-indolylcarboxylic acid; C017408:6-hydroxy-5-methoxy-2-indolylcarboxylic acid