Effect of castration on eicosanoid and catecholamine-induced luteinizing hormone-releasing hormone release in vitro.
The castration of adult rats four weeks before experimentation did not alter either the basal production of prostaglandin (PG) E2 (intact rats 1157 +/- 160 pg/mg protein, castrated rats 1093 +/- 90 pg/mg protein) or the A 23187-induced production of PGE2 (1591 +/- 209 or 1701 +/- 286 pg/mg protein respectively) or that of leucotriene (LT) C4 (474 +/- 33, 389 +/- 39 pg/mg protein). Castration significantly reduced the absolute amounts of luteinizing hormone-releasing hormone (LHRH) released under basal conditions (594 +/- 56 pg/mg protein to 34% of intact controls) and after A 23187 stimulation (to 28% of controls). The LHRH released by the median eminence (ME) of castrated rats in the presence of PGE2 (10(-6) M), LTC4 (10(-8) M), norepinephrine (NE) (10(-5) M) or dopamine (DA) (10(-5) M and 10(-4) M) was significantly lower than the LHRH released by intact animals in the presence of these factors. However the relative values for LHRH release (e.g. as % of controls) indicated that the degree of stimulation was identical in intact and castrated rats (2-3 times). These data suggest that the absence of gonadal steroids resulting from long-term castration does not block the stimulatory action of eicosanoids or catecholamines on the release of LHRH by the ME of adult male rats in vitro. Furthermore, castration does not interfere with the biosynthesis of PGE2 and LTC4.
Gerozissis
K
K
INSERM U. 207, URIA Institut Pasteur, Paris, France.
Saadi
M
M
Dray
F
F
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002395:Catecholamines / Q000494:pharmacology*; D015232:Dinoprostone / Q000096:biosynthesis* / Q000494:pharmacology; D004298:Dopamine / Q000494:pharmacology; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D008297:Male; D008473:Median Eminence / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology; D009919:Orchiectomy; D051381:Rats; D011919:Rats, Inbred Strains; D013189:SRS-A / Q000096:biosynthesis* / Q000494:pharmacology
D002395:Catecholamines; D013189:SRS-A; D007987:Gonadotropin-Releasing Hormone; D000001:Calcimycin; D015232:Dinoprostone; D004298:Dopamine; D009638:Norepinephrine