The effect of inhibitors of DNA synthesis on 40-kilodalton polypeptide translation in rat L6 myoblasts.
Messenger RNA coding for a polypeptide of 40 kilodaltons (P40) was translated in proliferating rat L6 myoblasts but not in the terminally differentiated myotubes. The relationship between DNA synthesis, differentiation, and P40 mRNA translation was studied. Aphidicolin, a reversible inhibitor of DNA synthesis, was shown to block DNA synthesis in proliferating myoblasts without allowing these cells to differentiate. A second inhibitor, cytosine arabinoside, when added to dividing myoblasts also prevented differentiation. In the absence of biochemical differentiation P40 mRNA remained in the translated state. Translational repression of this mRNA was, therefore, linked to the biochemical differentiation of rat L6 myoblasts.
Meadus
W J
WJ
University of Guelph, Department of Molecular Biology and Genetics, Ont., Canada.
Bag
J
J
D000818:Animals; D016590:Aphidicolin; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D003561:Cytarabine / Q000494:pharmacology*; D004261:DNA Replication / Q000187:drug effects*; D004224:Diterpenes / Q000494:pharmacology*; D005786:Gene Expression Regulation; D009132:Muscles / Q000166:cytology*; D010452:Peptide Biosynthesis; D014176:Protein Biosynthesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats
D004224:Diterpenes; D012333:RNA, Messenger; D003561:Cytarabine; D016590:Aphidicolin