[Biochemical changes in the brain tissue of white rats exposed to the herbicide acetochlor under acute and subacute oral experimental conditions].
In the conditions of acute and subacute oral intoxication with acetanilidic preparation acetochlorine applied in doses respectively 1/5 LD50 for acute experiment and 1/20 and 1/100 LD50 for subacute experiment (LD50 for male animals--1063 mg/kg-1, and for females--768 mg/kg-1) are traced the changes in the capacity for methaemoglobin formation, the quantity of sulfhydryl groups in the serum, the activity of LDH, G-6-PhDH, IDH, MDH, SDH, Cyt Oxidasis and the quantity of soluble proteins in brain homogenates and isolated mitochondria of brain. The animals of the acute experiment are examined on the 1, 3, 5, 7, 10 and 14 day of the poisoning, and the animals of the subacute experiment--on the 24th hour after the last administration (42-day treatment with the preparation, 5 days weekly). The herbicide acetochlorine at unrepeated introduction of dose 212 6 mg/kg-1 (1/5 LD50) and multiple administration for a period of 42 days in doses 1/20 and 1/1000 LD50 in the stomach of white rats possess well expressed methaemoglobin formation capacity. In the conditions of acute and subacute experiment the preparation causes one-type changes in the enzymes activity, certifying for a destruction of the mitochondrial apparatus. A suppression of the activity of SDH, IDH, Cyt oxidasis and increase of the LDH, G-6-PhDH activity, could be a result of methaemoglobin formation. The latter is a predispose factor for appearing of chemical hypoxia leading to the inclusion of compensatory anaerobic oxidizing processes. The registered deviations have to be read at the hygiene standardization of the preparation.
Khalkova
Zh
Zh
Zaĭkov
Kh
Kh
Buzidi
A
A
Antov
G
G
D000284:Administration, Oral; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006540:Herbicides / Q000633:toxicity*; D007928:Lethal Dose 50; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014052:Toluidines / Q000633:toxicity*
D006540:Herbicides; D014052:Toluidines; C043377:acetochlor
Biokhimichni promeni v mozŭchnata tŭkan na beli plŭkhove pri vŭzdeĭstvieto na kherbitsida atsetokhlor v usloviiata na ostŭr i podostŭr oralen eksperiment.