Regulation of the class II-associated invariant chain gene in normal and mutant B lymphocytes.
The invariant chain protein is intracellularly associated with class II major histocompatibility proteins. In many cases, the expression of these molecules appears to be regulated in a similar manner. Contained within the promoter of the invariant chain gene are sequences (X and I gamma 1) that are similar to the X and Y box elements of class II genes, suggesting that these sequences might be involved in its regulation. DNase I footprinting reveals additional cis-acting elements (I gamma 2 and I gamma 3) that contain sequence similarities to NF-kappa B and/or H2TF1/KBF1 recognition sequences. A series of fusion constructs with the chloramphenicol acetyltransferase reporter gene were used to analyze the role of these sequences (I gamma 1, I gamma 2, I gamma 3, and X and Y elements) in both normal and mutant B lymphocytes. These data suggest the likelihood of multiple X box proteins in B cells, which can act as both negative and positive regulatory factors.
Doyle
C
C
Division of Tumor Virology, Dana-Farber Cancer Institute, Boston, MA 02115.
Ford
P J
PJ
Ponath
P D
PD
Spies
T
T
Strominger
J L
JL
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D002051:Burkitt Lymphoma; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D002872:Chromosome Deletion; D004273:DNA, Neoplasm / Q000235:genetics; D003850:Deoxyribonuclease I; D005786:Gene Expression Regulation; D005802:Genes, MHC Class II; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D009710:Nucleotide Mapping; D015345:Oligonucleotide Probes; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D004273:DNA, Neoplasm; D000949:Histocompatibility Antigens Class II; D046911:Macromolecular Substances; D015345:Oligonucleotide Probes; D015500:Chloramphenicol O-Acetyltransferase; D003850:Deoxyribonuclease I