Covalent immobilization of biomolecules onto polystyrene MicroWells for use in biospecific assays.
Modification of polystyrene for higher binding capacity and/or for specific covalent immobilization of biomolecules is discussed. The benefit of covalent coupling of biomolecules onto a new commercially available surface type for covalent immobilization, CovaLink NH, is illustrated. The CovaLink NH solid phase has spacer arms covalently grafted onto the polystyrene solid phase, approximately 10(14) groups/cm2. Coupling procedures for covalent immobilization of biotin and peptides are demonstrated, and the advantage of using carbodiimide for coupling of carboxylic acid containing compounds is shown.
Rasmussen
S E
SE
NUNC Laboratories, Ros-Kilde, Denmark.
D001672:Biocompatible Materials; D001681:Biological Assay / Q000379:methods*; D001710:Biotin / Q000032:analysis; D002234:Carbodiimides; D010455:Peptides / Q000032:analysis; D011137:Polystyrenes
D001672:Biocompatible Materials; D002234:Carbodiimides; D010455:Peptides; D011137:Polystyrenes; D001710:Biotin