title: Immunization of baboons with attenuated schistosomula of Schistosoma haematobium: levels of protection induced by immunization with larvae irradiated with 20 and 60 krad.
abstract: We have shown previously that baboons (Papio anubis) develop high levels (greater than 80%) of protection against challenge infection following immunization with Schistosoma haematobium cercariae irradiated with 20 krad. In the present study baboons were immunized with schistosomula irradiated with either 20 krad or 60 krad, with variations in the timing and number of larvae comprising each vaccination. Baboons immunized 2 or 3 times with schistosomula irradiated with 20 krad were significantly more protected (85-90%) against challenge infection than baboons similarly immunized with larvae receiving 60 krad (56-50% protection). Baboons immunized with schistosomula irradiated with 20 krad were better protected against challenge infection at 8 weeks after immunization than at 28 weeks after immunization. Protection was manifest by a reduction in worm numbers, tissue and excreta egg counts, gross pathology and, to a lesser extent, by stability of body weight and haematological indices following challenge. Enzyme-linked immunosorbent assay (ELISA) results of selected baboon sera showed few differences related to irradiation dose alone, but titres were higher in baboons receiving booster immunizations, and there was a significant correlation between titres immediately preceding challenge and the degree of resistance. Examination of responses to individual schistosomular surface antigens by immunoprecipitation and sodium dodecyl sulphate-polyacrylamide gel electrophoresis showed no correlation between the pattern of antigens recognized and resistance status. As with the ELISA assay, an anamnestic response was detected after vaccination, while the amount of antibody present declined markedly with increasing time after individual immunizations.
authors.affiliation: Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, UK.
authors.forename: R A
authors.initials: RA
authors.lastname: Harrison
authors.forename: Q D
authors.initials: QD
authors.lastname: Bickle
authors.forename: S
authors.initials: S
authors.lastname: Kiare
authors.forename: E R
authors.initials: ER
authors.lastname: James
authors.forename: B J
authors.initials: BJ
authors.lastname: Andrews
authors.forename: R F
authors.initials: RF
authors.lastname: Sturrock
authors.forename: M G
authors.initials: MG
authors.lastname: Taylor
authors.forename: G
authors.initials: G
authors.lastname: Webbe
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D004307:Dose-Response Relationship, Radiation; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D007114:Immunization; D007074:Immunoglobulin G / Q000032:analysis; D010215:Papio / Q000469:parasitology; D010270:Parasite Egg Count; D012548:Schistosoma haematobium / Q000276:immunology; D012553:Schistosomiasis haematobia / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*; D013997:Time Factors
chemical_list: D000909:Antibodies, Helminth; D007074:Immunoglobulin G