Transcription analysis of class II human leukocyte antigen genes from normal and immunodeficient B lymphocytes, using polymerase chain reaction.
The RNA transcript levels of all human leukocyte antigen class II loci were determined from class II congenital immunodeficient B cells by polymerase chain reaction amplification of cDNA. No mRNA was observed under conditions in which 0.01% normal levels could be visualized. Pre-mRNA could be amplified from normal B cells but not from immunodeficient B cells, indicating a transcription defect.
Bull
M
M
Immunogenetics Research Section, Blood Center of Southeastern, Wisconsin, Milwaukee 53233.
van Hoef
A
A
Gorski
J
J
D001402:B-Lymphocytes / Q000201:enzymology*; D001483:Base Sequence; D002460:Cell Line; D005802:Genes, MHC Class II; D006801:Humans; D007153:Immunologic Deficiency Syndromes / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012322:RNA Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012016:Reference Values; D014158:Transcription, Genetic
D015345:Oligonucleotide Probes; D012322:RNA Precursors; D012333:RNA, Messenger