In-vitro preparation of experimental models of hepatitis with D-galactosamine and their modification by liver-repairing factors.
Hepatocytes, isolated from male F344/DuCrj rats by means of perfusion with collagenase, were incubated in Williams E medium containing newborn calf serum (PFCS), insulin, dexamethasone and D-galactosamine. The resulting liver-cell damage was confirmed by MTT assay to be dependent on the dose of D-galactosamine. This damage was clearly modified by the addition to the medium of the liver-repairing factors which appeared in serum in the initial stage of liver damage resulting from either partial hepatectomy or administration of D-galactosamine (Gal-N) in rats. When a massive number of splenocytes from normal rats was injected into the caudal vein of rats with liver damage caused by D-galactosamine, liver-damage-repairing factors were induced in the serum which were found to exert a particularly strong inhibition of the damage to hepatocytes caused by D-galactosamine when incubated in vitro.
Takahashi
N
N
Division of Agricultural Chemistry, Faculty of Agriculture, Meiji University, Kanagawa, Japan.
Ishizuya
T
T
Mori
N
N
D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D005688:Galactosamine / Q000494:pharmacology; D003699:Hepatitis D / Q000139:chemically induced* / Q000473:pathology; D007275:Injections, Intravenous; D008099:Liver / Q000473:pathology / Q000503:physiopathology*; D008115:Liver Regeneration / Q000502:physiology*; D051381:Rats / Q000097:blood; D011919:Rats, Inbred Strains; D013154:Spleen / Q000166:cytology / Q000637:transplantation
D003470:Culture Media; D005688:Galactosamine