Genetic transformation and cell morphology of Bacillus subtilis grown in Mg+(+)-limited chemostat culture.
The rate and frequency of genetic transformation of Bacillus subtilis grown in Mg+(+)-limited chemostat culture are dependent on the dilution rate (D) of the system and achieved maximum values at D = 0.23 h-1. Mg+(+)-limitation induced a morphological change in the cells from their normal rod shape to extended helices. Although this change in shape was a transient phenomenon, under some conditions it persisted for several days and resulted in an apparent increase in the transformation frequency.
Sevinc
M S
MS
Division of Biosphere Sciences, King's College, University of London, Great Britain.
Bainbridge
B W
BW
Bazin
M J
MJ
D001412:Bacillus subtilis / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D003470:Culture Media; D008274:Magnesium / Q000378:metabolism*; D008345:Manganese / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D014169:Transformation, Bacterial
D003470:Culture Media; D008345:Manganese; D008274:Magnesium