5-series peptido-leukotriene synthesis in mouse peritoneal macrophages: modulation by dietary n-3 fatty acids.
This study was undertaken to elucidate the metabolic fate of dietary eicosapentaenoic acid (20:5n-3), a major n-3 polyunsaturated fatty acid constituent of fish oil, in the mouse peritoneal macrophage. Mice were fed diets containing menhaden fish oil (FO) or safflower oil (SO). After 3 weeks, resident or responsive peritoneal macrophages were isolated and stimulated with zymosan and A23187, respectively. We demonstrate the novel synthesis of leukotriene C5 (LTC5), derived from 20:5n-3, in addition to LTC4 in both resident and responsive macrophages from FO fed mice. The structures of these peptido-leukotrienes were characterized by their retention times on reverse phase high pressure liquid chromatography, by their u.v. absorbance spectra, and by cross-reactivity with peptido-leukotriene antibody. These results demonstrate for the first time that macrophages are capable of metabolizing dietary 20:5n-3 to LTC5, a pentaene peptido-leukotriene.
Chapkin
R S
RS
Department of Animal Science, Texas A&M University, College Station 77843-2471.
Hubbard
N E
NE
Erickson
K L
KL
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004041:Dietary Fats / Q000378:metabolism*; D015118:Eicosapentaenoic Acid / Q000378:metabolism*; D005260:Female; D005395:Fish Oils / Q000378:metabolism*; D015289:Leukotrienes / Q000096:biosynthesis* / Q000302:isolation & purification; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D010938:Plant Oils / Q000378:metabolism*; D012450:Safflower Oil / Q000378:metabolism*; D013864:Thioglycolates / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D004041:Dietary Fats; D005395:Fish Oils; D015289:Leukotrienes; D010938:Plant Oils; D013864:Thioglycolates; C033266:Menhaden oil; D000001:Calcimycin; D012450:Safflower Oil; D015054:Zymosan; D015118:Eicosapentaenoic Acid