Measurement of lithium transport in RBC from psychiatric patients receiving lithium carbonate and normal individuals by 7Li NMR spectroscopy.
A reproducible 7Li nuclear magnetic resonance (NMR) method, based on a modified inversion recovery (MIR) pulse sequence, was used to discriminate between intra- and extracellular lithium concentrations in red blood cell (RBC) suspensions. The rates of Na(+)-Li+ countertransport determined by the 7Li NMR method were significantly correlated with the measurements made by atomic absorption (AA) for 14 psychiatric patients receiving lithium carbonate (r = 0.937) and 14 normal individuals (r = 0.931). As expected, the rates of Na(+)-Li+ countertransport measured by MIR were significantly lower for the psychiatric patients receiving lithium carbonate than for normal individuals. The 7Li NMR method provides RBC Li+ countertransport information comparable to AA for psychiatric patients and normal individuals. A description of the advantages of the 7Li NMR method in contrast to the AA method, including the study of Li+ interactions with RBC components such as membrane proteins and anionic phospholipids, is included.
Mota de Freitas
D
D
Department of Chemistry, Loyola University of Chicago, IL 60626.
Silberberg
J
J
Espanol
M T
MT
Dorus
E
E
Abraha
A
A
Dorus
W
W
Elenz
E
E
Whang
W
W
D000328:Adult; D001714:Bipolar Disorder / Q000097:blood* / Q000188:drug therapy; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006801:Humans; D008094:Lithium / Q000493:pharmacokinetics* / Q000627:therapeutic use; D016651:Lithium Carbonate; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D008875:Middle Aged; D012964:Sodium / Q000097:blood
D016651:Lithium Carbonate; D008094:Lithium; D012964:Sodium