title: Monocyte activation by smooth muscle cell-derived matrices.
abstract: Mononuclear phagocytes adhere to and penetrate the vessel wall endothelium and contact the subendothelial space prior to the development of the atherosclerotic plaque. In an attempt to model the early events of plaque development we used an elastin-rich, multicomponent, cell-derived matrix from neonatal rat aortic smooth muscle cells as a substratum for monocytes. Using this model, we show that human monocyte morphology and metabolism are markedly altered by the matrix substratum. When a mixed mononuclear cell population is seeded on matrix or plastic, only monocytes adhere to the matrix surface. In contrast, lymphocytes as well as monocytes adhere to the plastic surface. The matrix-adherent monocytes develop large intracellular granules and form extensive clusters of individual cells. Metabolically, these cells develop sodium fluoride resistant non-specific esterase activity and their media contain more growth factor activity and PGE2. Although total protein synthesis is equivalent in both cultures, the matrix contact induces an increase in specific proteins in the media. We also show that a purified alpha-elastin substratum induces some, but not all, of the monocyte changes seen when using the matrix substratum. Using the alpha-elastin substratum, there is selective adhesion of monocytes and increased growth factor activity, however, the cells are morphologically different from the matrix-adherent cells. Thus, the use of the smooth muscle cell-derived matrix, in conjunction with purified matrix components, serves as a model that can provide insight into the mechanisms of monocyte adhesion and stimulation by the matrix environment that exists in vivo. Such mechanisms may be particularly important in atherogenesis.
authors.affiliation: Department of Biochemistry, Boston University School of Medicine, MA 02118.
authors.forename: J
authors.initials: J
authors.lastname: Kaufmann
authors.forename: R W
authors.initials: RW
authors.lastname: Jorgensen
authors.forename: B M
authors.initials: BM
authors.lastname: Martin
authors.forename: C
authors.initials: C
authors.lastname: Franzblau
mesh_terms: D000818:Animals; D001011:Aorta / Q000502:physiology; D001161:Arteriosclerosis / Q000378:metabolism / Q000503:physiopathology; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D002417:Cattle; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D015232:Dinoprostone / Q000096:biosynthesis; D004549:Elastin / Q000502:physiology; D005109:Extracellular Matrix / Q000502:physiology*; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D009000:Monocytes / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D009131:Muscle, Smooth, Vascular / Q000502:physiology*; D010969:Plastics; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D010969:Plastics; D004549:Elastin; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; D015232:Dinoprostone