title: [A decrease in sister chromatid exchange frequency during the prolonged cultivation of human lymphocytes].
abstract: Sister chromatid exchange (SCE) frequency at different times of fixation was studied in human lymphocyte cultures obtained from 6 donors. No differences were found in the SCE frequency between human lymphocyte cultures fixed at 72 and 96 hours of incubation (10.61 +/- 0.85 and 10.15 +/- 0.81 SCE per cell, respectively). However, a decreased SCE frequency (8.11 +/- 0.36 SCE per cell) was observed in cultures fixed at 120 hours of incubation. For a more detailed studies, one lymphocyte culture was fixed at different times of incubation (from 56 to 128 hours, at each a 8 hours). A slight increase in SCE frequencies was found at the interval between 56 and 88 hours of incubation, while starting from 104 hours of incubation a marked decrease in the SCE frequency was observed. Time-dependent changes in the SCE frequency may be described by the equation y = -1.8614 + 0.3922x - (2.5183 x 10(-3))x2, where y is the number of SCEs per cell, and x--the duration of culture incubation in hours. The observed phenomenon may be associated with changes in proportion of T and B lymphocytes, or with heterochromatization of chromosomes during a prolonged cultivation, or with an early in vitro stimulation of the in vivo long-lived lymphocytes that may be more damaged than the in vivo short-lived and the in vitro late-stimulating ones.
authors.forename: Iu S
authors.initials: IuS
authors.lastname: Lazutka
authors.forename: V V
authors.initials: VV
authors.lastname: Dedonite
mesh_terms: D000328:Adult; D002478:Cells, Cultured / Q000648:ultrastructure; D003470:Culture Media; D003584:Cytological Techniques; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure*; D008297:Male; D008677:Metaphase; D012854:Sister Chromatid Exchange; D013997:Time Factors
chemical_list: D003470:Culture Media
vernacular_title: Snizhenie chastoty sestrinskikh khromatidnykh obmenov pri dlitel'nom kul'tivirovanii limfotsitov cheloveka.