Patterns and extent of isotype-specificity in the murine H chain switch DNA rearrangement.
We have analyzed the configuration of the H chain locus of 41 hybridomas by Southern blot analysis. Each H chain switch region was determined to be germ line, rearranged, or deleted. Including 13 previously analyzed hybridomas, 60% of those with rearrangements on both alleles showed a correlation of the two alleles, i.e., both the expressed and the nonexpressed alleles have rearranged to the same H chain constant region gene segment. When the two H chain alleles did not rearrange to the same gene, they often rearranged to neighboring H chain genes. These results support a role for isotype-specific factors in H chain switch recombination. The action of these isotype-specific factors may be propagated to some extent along the chromosome, which would lead to rearrangements to neighboring genes.
Schultz
C
C
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109-0620.
Petrini
J
J
Collins
J
J
Claflin
J L
JL
Denis
K A
KA
Gearhart
P
P
Gritzmacher
C
C
Manser
T
T
Shulman
M
M
Dunnick
W
W
D000483:Alleles; D000818:Animals; D015139:Blotting, Southern; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D005803:Genes, Immunoglobulin; D006825:Hybridomas; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007132:Immunoglobulin Isotypes / Q000235:genetics; D051379:Mice
D007143:Immunoglobulin Heavy Chains; D007132:Immunoglobulin Isotypes