title: Reperfusion injury in ischemic myocardium: protective effect of controlled reperfusion.
abstract: Restoration of coronary artery flow following a period of ischemia often results in further ultrastructural damage to cardiac fibers, a phenomenon known as reperfusion injury. We have compared the ultrastructural effects of uncontrolled reperfusion in vivo of ischemic pig myocardium with the ultrastructural effects of reperfusion controlled at flow rates comparable to preischemia levels. Myocardial ischemia was produced for 60 minutes in 9 pigs by means of a reversible coronary artery occlusion, after which coronary artery flow was restored for 120 minutes. This restoration of flow was complete in four pigs (resulting in uncontrolled reperfusion) and partial in five pigs, with constant monitoring and adjustment of flow to maintain rates near preischemia values (controlled reperfusion). Myocardial samples from the ischemic, reperfused region were examined by electron microscopy. Ischemic damage to nuclei, mitochondria, and myofibrils and ischemic depletion of glycogen were graded independently and blindly by two investigators using a simple, nonparametric three-point scale. Ischemic damage was greater in pigs receiving uncontrolled reperfusion than in animals receiving controlled reperfusion, and these differences were significant for ischemic effects on nuclei (p less than 0.01), glycogen (p less than 0.02), and myofibrils (p less than 0.05) but not for ischemic effects on mitochondria (p = 0.095). We conclude that uncontrolled, hyperemic flow during reperfusion of ischemic myocardium is responsible, in part, for the phenomenon of reperfusion injury.
authors.affiliation: Division of Pathology, John L. McClellan Memorial Veterans Hospital, Little Rock, AR.
authors.forename: R E
authors.initials: RE
authors.lastname: Mrak
authors.forename: M M
authors.initials: MM
authors.lastname: Carry
authors.forename: M L
authors.initials: ML
authors.lastname: Murphy
authors.forename: C F
authors.initials: CF
authors.lastname: Peng
authors.forename: K D
authors.initials: KD
authors.lastname: Straub
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D003326:Coronary Circulation / Q000502:physiology; D008854:Microscopy, Electron; D008929:Mitochondria, Heart / Q000648:ultrastructure; D015425:Myocardial Reperfusion / Q000379:methods*; D015428:Myocardial Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D009206:Myocardium / Q000648:ultrastructure*; D013552:Swine; D013997:Time Factors