Regulation of sialyltransferase activity in intestinal segments of rats.
A differential distribution of sialyltransferase (ST) in different regions of intestine has been shown. Jejunum and ileum homogenates from rats showed almost exclusive presence of alpha-2-3 ST (to Gal in Gal beta-1-4GlcNAc and/or to Gal in Gal beta-1-3GalNAc). In contrast, colon homogenates showed the presence of both alpha-2-3 ST (as above) and alpha-2-6 ST. Incubation of intestinal slices in presence of heat-inactivated horse serum (HHS) showed a time- and temperature-dependent secretion of soluble ST into the medium. Both jejunum and ileum slices showed high rates of secretion of alpha-2-3 ST. Colon slices, though rich in alpha-2-6 ST, secreted only alpha-2-3 ST. Colchicine, an anti-mitotic drug, injected into rats caused about 10-fold increase of the serum ST level. Jejunum slices from colchicine-treated rats showed an increased secretion of alpha-2-6 ST, suggesting that intestine undergoes a change in the expression of normal secretion of alpha-2-3 ST to a secretion of alpha-2-6 ST. The secretion of ST from incubated intestinal slices was inhibited by heparin. Certain protein factors (anti-proteases) in HHS bind to heparin-sepharose column and these protein factors are responsible for causing the secretion of ST into the medium. It has also been found that a supernatant fraction of the colon homogenate activated ST. Gel chromatography on HPLC produced 3-4 protein fractions from the colon cytosol and one of this fraction bearing high molecular weight proteins produced the maximum activation of ST.(ABSTRACT TRUNCATED AT 250 WORDS)
Mookerjea
S
S
Department of Biochemistry, Memorial University of Newfoundland, St. John's, Canada.
Hunt
D
D
Nadkarni
S
S
Ratnam
S
S
Collins-Francis
J
J
Nagpurkar
A
A
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D003078:Colchicine / Q000494:pharmacology; D003106:Colon / Q000187:drug effects / Q000201:enzymology*; D003600:Cytosol / Q000201:enzymology; D004789:Enzyme Activation; D006493:Heparin / Q000494:pharmacology; D007082:Ileum / Q000187:drug effects / Q000201:enzymology*; D007583:Jejunum / Q000187:drug effects / Q000201:enzymology*; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012799:Sialyltransferases / Q000378:metabolism*; D013696:Temperature; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
D006493:Heparin; D012799:Sialyltransferases; D003078:Colchicine; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase