Gamma-radiation and mitomycin C sensitivity of peripheral blood lymphocytes from Saudi patients with non-Hodgkin's lymphoma.
Peripheral blood lymphocytes (PBLs) from 28 patients with non-Hodgkin's lymphoma (NHL) were compared with those of 28 healthy subjects (controls) for their responsiveness to the T cell mitogen phytohemagglutinin (PHA) before and after treatment with gamma-radiation and mitomycin C. PBLs from 9 of the 28 patients with NHL exhibited almost a total failure to respond to PHA while mitogenic stimulation in the rest varied from 7 to 90% relative to the controls. PBLs from 19 NHL patients and 28 healthy subjects were compared for their radiosensitivity by measuring postirradiation uptake of [3H]-thymidine. Increased sensitivity to gamma-radiation was observed in more than half of the NHL patients studied. Increased sensitivity to mitomycin C was also noted in 5 of the 7 patients analyzed compared to the respective controls. Poor mitogenic responsiveness and hypersensitivity of blood lymphocytes to the carcinogens (gamma-rays and mitomycin C) are suggestive of a severe immunological abnormality and defective DNA repair in these Saudi NHL patients.
Hannan
M A
MA
Department of Biological, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
al-Sedairy
S T
ST
Gibson
D P
DP
McGarry
T J
TJ
Amer
M H
MH
D004261:DNA Replication / Q000187:drug effects / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005720:Gamma Rays; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects / Q000528:radiation effects; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects*; D008228:Lymphoma, Non-Hodgkin / Q000097:blood / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology*; D012016:Reference Values; D013936:Thymidine / Q000378:metabolism
D008937:Mitomycins; D016685:Mitomycin; D013936:Thymidine