[Vasopressin in tissue basophils of the dura mater of white rats].
By an indirect immunohistochemical method with fluorescein-isothiocyanate (FITC) and horse radish peroxidase as markers (HRP) the presence of vasopressin was shown in cells of dura mater in white rats. Mast cells were identified after staining with methylene blue by the metachromatic granularity of the cytoplasm. It was shown that the number of cells found by means of FITC luminescence corresponds with their number found by means of methylene blue. The use of conjugate with HRP unveils a lesser number of vasopressin-containing cells.
Lomakin
A V
AV
Kan
G A
GA
Motavkin
P A
PA
D000818:Animals; D004388:Dura Mater / Q000032:analysis* / Q000166:cytology; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D006735:Horseradish Peroxidase; D007150:Immunohistochemistry; D008163:Luminescent Measurements; D008407:Mast Cells / Q000032:analysis; D008751:Methylene Blue; D051381:Rats; D013861:Thiocyanates; D014667:Vasopressins / Q000032:analysis*
D005452:Fluoresceins; D013861:Thiocyanates; D014667:Vasopressins; D006735:Horseradish Peroxidase; D016650:Fluorescein-5-isothiocyanate; D008751:Methylene Blue
Vazopressin v tkanevykh bazofilakh tverdoĭ mozgovoĭ obolochi belykh krys.