Interferon-alpha/beta and Rickettsia prowazekii: induction and sensitivity.
Rickettsia prowazekii Madrid E established persistent infections in cultures of growing L-929 cells. Although some L-929 cells died, the cultures survived, remained infected with rickettsiae, and continued to grow. R. prowazekii Madrid E also induced interferon in L-929 cell cultures, and this interferon modulated rickettsial growth. Production of interferon (anti-viral activity) by cultures of R. prowazekii-infected L-929 cells was directly related to the initial rickettsial infection and was blocked by erythromycin. The media collected from R. prowazekii-infected L-929 cells suppressed not only the replication of vesicular stomatitis virus but also the growth of R. prowazekii in fresh L-929 cells. Both anti-viral and anti-rickettsial activities in the media were neutralized by antibodies against murine interferons-alpha and -beta, but not by antibodies against murine interferon-gamma. In addition, a commercial preparation of virus-induced interferons-alpha and -beta also suppressed rickettsial growth in L-929 cells. The combination of treating L-929 cells with this virus-induced interferon and infecting them with R. prowazekii killed some of the L-929 cells.
Turco
J
J
Department of Microbiology and Immunology, College of Medicine, University of South Alabama, Mobile 36688.
Winkler
H H
HH
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D007370:Interferon Type I / Q000096:biosynthesis / Q000502:physiology*; D051379:Mice; D012283:Rickettsia prowazekii / Q000187:drug effects / Q000254:growth & development*
D003470:Culture Media; D007370:Interferon Type I