title: An immunochemical evaluation for the identification and typing of monoclonal proteins.
abstract: Using automated measurements of kappa (kappa) and lambda (lambda) light chain and IgG, IgA and IgM concentrations we assessed the ability of the kappa/lambda ratio and the heavy/light chain ratio to identify and type monoclonal components which had been previously identified by serum protein electrophoresis and immunoelectrophoresis. We examined 1151 samples, of which 277 contained monoclonal components; 90% of the monoclonal proteins could be identified by this approach, of which 95% were correctly typed. In addition 19 of 874 samples without monoclonal components showed an abnormal kappa/lambda ratio. We assessed the ability of the immunochemical evaluation to calculate monoclonal component concentration and then compared the results to the scanning densitometry of serum protein electrophoresis.
authors.affiliation: Supraregional Protein Reference Unit, Royal Infirmary, Cardiff, Wales, UK.
authors.forename: R
authors.initials: R
authors.lastname: Fifield
authors.forename: I
authors.initials: I
authors.lastname: Keller
mesh_terms: D001797:Blood Protein Electrophoresis; D003720:Densitometry; D006801:Humans; D007120:Immunochemistry; D007122:Immunoelectrophoresis; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007143:Immunoglobulin Heavy Chains / Q000032:analysis*; D007147:Immunoglobulin Light Chains / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis; D007145:Immunoglobulin kappa-Chains / Q000032:analysis*; D007146:Immunoglobulin lambda-Chains / Q000032:analysis*; D012016:Reference Values
chemical_list: D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains