Characterization of a chemically synthesized RNA having the sequence of the yeast initiator tRNA(Met).
A 75-unit long oligoribonucleotide corresponding to the sequence of the Saccharomyces cerevisiae initiator tRNA was synthesized chemically. The crude RNA was purified, and the sequence was verified by RNA sequencing techniques. A particularly useful purification step involved hydrophobic chromatography on BND-cellulose. The purified RNA could be aminoacylated to 28% of a bona fide initiator tRNA(Met) sample and threonylated to 76% of the level observed with native tRNA(fMet) from E. coli.
Bratty
J
J
Département de Biochimie, Université de Montréal, Qué, Canada.
Wu
T F
TF
Nicoghosian
K
K
Ogilvie
K K
KK
Perreault
J P
JP
Keith
G
G
Cedergren
R
R
D001483:Base Sequence; D006860:Hydrogen Bonding; D008715:Methionine / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012345:RNA, Transfer, Amino Acid-Specific / Q000138:chemical synthesis*; D012358:RNA, Transfer, Met / Q000138:chemical synthesis* / Q000235:genetics / Q000648:ultrastructure; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013912:Threonine / Q000378:metabolism
D012345:RNA, Transfer, Amino Acid-Specific; D012358:RNA, Transfer, Met; D013912:Threonine; D008715:Methionine