Neuraminidase in juvenile calf thymus: determination and characterization by a continuous fluorometric assay procedure.
A continuous fluorometric neuraminidase assay has been developed. Within the pH range optimal for neuraminidases (from 3 to 6) the fluorescence intensity of 4-methylumbelliferone exceeds that of the glycoside 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid about 50 times (at lambda Ex = 335 nm and lambda Em = 445 nm), allowing the precise, simple and time-saving continuous fluorometric registration of enzymatically released 4-methylumbelliferone. In juvenile calf thymus a neuraminidase consisting of two components differing in pH optima and resistence to freezing and to detergents could be found. A beta-galactosidase activity in juvenile calf thymus could be proved by the same assay procedure using the synthetic substrate 4-methylumbelliferyl-beta-D-galactopyranoside.
Bieringer
K
K
Fachbereich 3, Theoretische Medizin, Universität des Saarlandes, Homburg/Saar, FRG.
Zoch
E
E
D000818:Animals; D002417:Cattle; D004795:Enzyme Stability; D005470:Fluorometry; D005615:Freezing; D006863:Hydrogen-Ion Concentration; D006923:Hymecromone / Q000378:metabolism; D007700:Kinetics; D008722:Methods; D009439:Neuraminidase / Q000032:analysis* / Q000378:metabolism; D010740:Phospholipases; D012995:Solubility; D013950:Thymus Gland / Q000201:enzymology*; D001616:beta-Galactosidase / Q000032:analysis
D006923:Hymecromone; D010740:Phospholipases; D009439:Neuraminidase; D001616:beta-Galactosidase