Total chemical assembly of genes.
An artificial gene comprising 183 base pairs has been assembled by template-directed condensation of 35- to 53-membered oligodeoxyribonucleotides with cyanogen bromide as a condensing agent. The reaction is complete within several minutes at 0 degree C in buffer. The resulting gene was cloned into M13mp11 and sequenced using the Sanger sequencing procedure.
Shabarova
Z A
ZA
Chemistry Department, Moscow State University, USSR.
Sokolova
N I
NI
Merenkova
I N
IN
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003488:Cyanogen Bromide; D004247:DNA; D005813:Genes, Synthetic; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D015183:Restriction Mapping; D013698:Templates, Genetic
D009838:Oligodeoxyribonucleotides; D004247:DNA; D003488:Cyanogen Bromide