title: Association of Ig L chain-like protein lambda 5 with a 16-kilodalton protein in mouse pre-B cell lines is not dependent on the presence of Ig H chain protein.
abstract: Using a polyclonal rabbit antiserum against recombinant mouse lambda 5 protein, we determined that the pre-B cell specific mouse lambda 5 gene encodes a 22-kDa protein. The lambda 5 protein, which is related to conventional Ig lambda L chain proteins forms a complex with Ig mu H chain protein and an as yet unidentified 16-kDa protein (p16) in mu+ pre-B cell lines carrying a functionally rearranged VH-DH-JH allele. In pre-B cell lines which carry DH-JH rearrangements and do not express mu H chain protein, lambda 5 protein is associated with p16. Thus the expression of lambda 5 protein precedes the expression of intact mu H chain protein. This suggests the existence of developmentally regulated protein complexes involving the Ig L chain-like protein lambda 5 and p16 in mu- pre-B cells; lambda 5, p16, and Ig H chain protein in mu+ pre-B cells and Ig H chain and conventional Ig L chain proteins in B cells and plasma cells.
authors.affiliation: Department of Immunology, University of Toronto, Ontario, Canada.
authors.forename: V
authors.initials: V
authors.lastname: Misener
authors.forename: J
authors.initials: J
authors.lastname: Jongstra-Bilen
authors.forename: A J
authors.initials: AJ
authors.lastname: Young
authors.forename: M J
authors.initials: MJ
authors.lastname: Atkinson
authors.forename: G E
authors.initials: GE
authors.lastname: Wu
authors.forename: J
authors.initials: J
authors.lastname: Jongstra
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000032:analysis*; D002460:Cell Line; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D006412:Hematopoietic Stem Cells / Q000032:analysis*; D007106:Immune Sera / Q000276:immunology; D007146:Immunoglobulin lambda-Chains / Q000032:analysis* / Q000276:immunology; D007148:Immunoglobulin mu-Chains / Q000032:analysis*; D051379:Mice
chemical_list: D007106:Immune Sera; D007146:Immunoglobulin lambda-Chains; D007148:Immunoglobulin mu-Chains