Apis mellifera cytoplasmic elongation factor 1 alpha (EF-1 alpha) is closely related to Drosophila melanogaster EF-1 alpha.
Using low stringency hybridisation with a Drosophila melanogaster EF-1 alpha gene fragment we have isolated a genomic DNA clone encoding elongation factor 1 alpha (EF-1 alpha) from Apis mellifera. The hybridising Apis mellifera sequence could be delineated to two small EcoRI fragments that were also revealed by genomic Southern hybridisation. By comparison with the corresponding Drosophila melanogaster data the complete translational reading frame has been deduced. It is interrupted by two intervening sequences of 220 and about 790 nucleotides. Comparison with known eucaryotic EF-1 alpha sequences further confirms that certain amino acid sequences seem to be invariable within the EF-1 alpha protein family.
Walldorf
U
U
Center for Molecular Biology Heidelberg, FRG.
Hovemann
B T
BT
D000595:Amino Acid Sequence; D000818:Animals; D001516:Bees / Q000235:genetics* / Q000378:metabolism; D005075:Biological Evolution; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000235:genetics*; D014176:Protein Biosynthesis; D013329:Structure-Activity Relationship
D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D004247:DNA