title: Purification and properties of a thermophilic and thermostable DNA polymerase from the archaebacterium Sulfolobus solfataricus.
abstract: A DNA-dependent DNA polymerase was obtained in homogenous form from the thermoacidophilic archaebacterium Sulfolobus solfataricus. The enzyme, purified 706-fold, has a molecular mass of about 110000 daltons as determined by gel filtration and by glycerol gradient centrifugation. It requires Mg++ for its activity and has a pH optimum of 7.7. The activity is sharply dependent on the ionic strength. The enzyme is thermostable; its properties and activity requirements were characterized. The features of this enzyme are compared to those of other DNA polymerases isolated either from prokaryotes or eukaryotes.
authors.affiliation: Istituto di Biochimica delle Proteine ed Enzimologia, CNR, Napoli.
authors.forename: R
authors.initials: R
authors.lastname: Rella
authors.forename: C A
authors.initials: CA
authors.lastname: Raia
authors.forename: F M
authors.initials: FM
authors.lastname: Pisani
authors.forename: S
authors.initials: S
authors.lastname: D'Auria
authors.forename: R
authors.initials: R
authors.lastname: Nucci
authors.forename: A
authors.initials: A
authors.lastname: Gambacorta
authors.forename: M
authors.initials: M
authors.lastname: De Rosa
authors.forename: M
authors.initials: M
authors.lastname: Rossi
mesh_terms: D001105:Archaea / Q000201:enzymology*; D001419:Bacteria / Q000201:enzymology*; D004259:DNA-Directed DNA Polymerase / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D008970:Molecular Weight; D019384:Nucleic Acid Synthesis Inhibitors; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D019384:Nucleic Acid Synthesis Inhibitors; D008345:Manganese; D004259:DNA-Directed DNA Polymerase; D008274:Magnesium