Characterization of a G-protein beta-subunit gene from the nematode Caenorhabditis elegans.
The gene encoding the beta-subunit of guanine nucleotide binding regulatory proteins (G-proteins) has been cloned from the nematode Caenorhabditis elegans. The predicted 340 amino acid sequence matches the highly conserved amino acid sequences of previously isolated G-protein beta-subunits from mammals and Drosophila. The coding region of the C. elegans beta-subunit gene, which has been mapped to the C. elegans chromosome II, is interrupted by eight introns. Southern analysis indicates that C. elegans has only one beta-subunit gene. A 2.8 kb (1 kb = 10(3) bases or base-pairs) transcript derived from this gene could be detected.
van der Voorn
L
L
Netherlands Cancer Institute, Amsterdam.
Gebbink
M
M
Plasterk
R H
RH
Ploegh
H L
HL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002107:Caenorhabditis / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D019204:GTP-Binding Proteins / Q000235:genetics*; D005796:Genes; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D004247:DNA; D019204:GTP-Binding Proteins