Further analysis on the structural proteins of infectious pancreatic necrosis virus.
The structural proteins of infectious pancreatic necrosis virus (IPNV) have been analyzed. Two-dimensional gel electrophoresis showed that IPNV proteins are slightly acidic with apparent pIs ranging from 5.8 to 6.6. To identify the IPNV surface-located proteins, purified virus was labelled either with fluorescein isothiocyanate (FITC) or with Na 125I. After analysis by SDS-PAGE, only the major viral protein, VP2, was labelled by either procedure. The accessibility of VP2 to these reagents suggests that this protein is externally located. In addition, using Concanavalin A conjugated with FITC and IPNV labelling with 3H-mannose, evidence is present that VP2 contains carbohydrate residues.
Estay
A
A
Laboratorio de Bioquimica, Facultad de Medicina, Universidad de Valparaiso, Chile.
Farias
G
G
Soler
M
M
Kuznar
J
J
D000818:Animals; D002213:Capsid / Q000032:analysis; D036022:Capsid Proteins; D003208:Concanavalin A / Q000031:analogs & derivatives; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives*; D005452:Fluoresceins; D006031:Glycosylation; D007526:Isoelectric Point; D009336:Necrosis; D010182:Pancreatic Diseases / Q000382:microbiology / Q000473:pathology; D012487:Salmonidae; D015678:Viral Structural Proteins / Q000032:analysis*; D014780:Viruses / Q000032:analysis*
D036022:Capsid Proteins; D005452:Fluoresceins; D015678:Viral Structural Proteins; C042784:fluorescein isothiocyanate-concanavalin A; D003208:Concanavalin A; D016650:Fluorescein-5-isothiocyanate