Variations of specific mRNA and polypeptide contents of rat liver D-beta-hydroxybutyrate dehydrogenase during an experimental diabetes mellitus.
The expression of the rat liver D-beta-hydroxybutyrate dehydrogenase (BDH) gene was investigated at different levels: the level of its specific mRNA, the protein content and the enzymatic activity. By using a cDNA probe, we found that the BDH mRNA was about 2 kb and we report here that the decrease of BDH activity in diabetic rats is due to a reduction in the content of the enzyme, which is proportional to a diminution in the amount of the BDH mRNA. We also show that insulin is able to reverse this diabetes effect by restoring the level of BDH mRNA, the BDH content and thus its activity. This result indicates that in vivo the control of the expression of the BDH gene by insulin is mainly transcriptional and/or post-transcriptional (mRNA stability).
Bailly
A
A
Laboratoire de Biochimie et Biologie Moléculaire, CNRS UA 531, Université de Franche-Comté, Besançon, France.
Lone
Y C
YC
Latruffe
N
N
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000235:genetics*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D014644:Genetic Variation; D006884:Hydroxybutyrate Dehydrogenase / Q000235:genetics*; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D006884:Hydroxybutyrate Dehydrogenase