title: Oxysterol mediated changes in fatty acid distribution and lipid synthesis in cultured bovine aortic smooth muscle cells.
abstract: We studied the actions of oxysterols on fatty acid distribution and lipid synthesis in cultured bovine aortic smooth muscle cells. Cultures were labeled with [1-14C]arachidonate or [1-14C]oleate. During a 24-hr incubation, 25- or 22R-hydroxycholesterol enhanced the incorporation of label into triglycerides, concomitant with a reduction in the labeling of phospholipids. Cholestantriol or 20-hydroxycholesterol had the opposite effects. They caused a higher incorporation of radiolabel into phospholipids and a reduction of labeling of triacylglycerols. Similar changes were seen in cells labeled with [1-14C]acetate. Therefore, we conclude that oxysterols can promote changes in the distribution of fatty acids between neutral lipids and phospholipids through mechanisms that still need to be clarified.
authors.affiliation: Laboratoire des Sciences de la Consommation, INRA-CRJ, Jouy en Josas, France.
authors.forename: C
authors.initials: C
authors.lastname: Seillan
mesh_terms: D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000818:Animals; D001011:Aorta; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D002777:Cholestanols / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism*; D006888:Hydroxycholesterols / Q000494:pharmacology*; D008055:Lipids / Q000096:biosynthesis*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis; D014280:Triglycerides / Q000096:biosynthesis
chemical_list: D000085:Acetates; D001095:Arachidonic Acids; D002777:Cholestanols; D005227:Fatty Acids; D006888:Hydroxycholesterols; D008055:Lipids; D009829:Oleic Acids; D010743:Phospholipids; D014280:Triglycerides; C000474:cholestane-3,5,6-triol; C003585:22-hydroxycholesterol; D016718:Arachidonic Acid; D019301:Oleic Acid; C025813:20-hydroxycholesterol; C007997:25-hydroxycholesterol; D019342:Acetic Acid