Enzymatic solid-phase assay for biotin and a biotin-benzodiazepine conjugate.
A novel enzymatic ligand binding assay for biotin and its benzodiazepine conjugate is based on their binding to horseradish peroxidase-avidin conjugate (A-P) followed by the uptake of biotin-unsaturated A-P onto polystyrene beads coated with biotin-BSA. The detection limit is 1.3 x 10(-16) mol per tube (300 microL) with a 3.3 x 10(-12) M A-P solution and varies with the conjugate concentration employed. The coefficient of variation for 10 repetitive assays of 10(-15) mol of biotin is 6.22%.
Takeuchi
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Department of Chemistry, University of Hawaii at Manoa, Honolulu 96822.
Rechnitz
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D001360:Avidin / Q000378:metabolism; D001569:Benzodiazepines / Q000032:analysis* / Q000378:metabolism; D001710:Biotin / Q000032:analysis* / Q000378:metabolism; D002623:Chemistry Techniques, Analytical; D006735:Horseradish Peroxidase / Q000378:metabolism; D011137:Polystyrenes; D012710:Serum Albumin, Bovine / Q000378:metabolism
D011137:Polystyrenes; D001569:Benzodiazepines; D001360:Avidin; D012710:Serum Albumin, Bovine; D001710:Biotin; D006735:Horseradish Peroxidase