title: Rapid and efficient detection of genetic polymorphism in wheat through amplification by polymerase chain reaction.
abstract: The polymerase chain reaction (PCR) was used to amplify genomic DNA of several wheat genotypes. The oligonucleotides used as primers were the terminal sequences of a gamma-gliadin gene. The electrophoretic analysis of the PCR products showed specific bands which revealed both inter- and intra-specific genetic polymorphism among the examined genotypes. The technique is proposed as a very simple and efficient alternative to RFLP markers.
authors.affiliation: Dipartimento di Agrobiologia e Agrochimica, Università della Tuscia, Viterbo, Italy.
authors.forename: R
authors.initials: R
authors.lastname: D'Ovidio
authors.forename: O A
authors.initials: OA
authors.lastname: Tanzarella
authors.forename: E
authors.initials: E
authors.lastname: Porceddu
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000235:genetics; D005784:Gene Amplification; D005819:Genetic Markers; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D014908:Triticum / Q000235:genetics
chemical_list: D005819:Genetic Markers; D004247:DNA