Effects of beta-hydroxy butyric acid on insulin binding to its receptor and on autophosphorylation of the receptor.
We examined the effects of beta-hydroxy butyric acid (B-OH-butyrate) on insulin binding to its receptor and on autophosphorylation of the receptor in vitro. The affinity of isolated rat adipocyte insulin receptors was significantly increased by B-OH-butyrate at neutral pH. Similar results were obtained with soluble insulin receptors prepared from hepatocytes. Autophosphorylation of insulin receptor, however, was not affected by OH-butyrate at neutral pH. These results suggested insulin resistance in ketoacidosis is not caused by an interaction between B-OH-butyrate and the insulin receptor.
Ohtsuka
H
H
Department of Geriatrics, Endocrinology and Metabolism, Shinshu University School of Medicine, Matsumoto, Japan.
Yamauchi
K
K
Ohara
N
N
Miyamoto
T
T
Ichikawa
K
K
Hashizume
K
K
D020155:3-Hydroxybutyric Acid; D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects; D000818:Animals; D001345:Autoradiography; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D006885:Hydroxybutyrates / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008297:Male; D010766:Phosphorylation; D051381:Rats; D011919:Rats, Inbred Strains; D011972:Receptor, Insulin / Q000187:drug effects* / Q000378:metabolism; D012995:Solubility
D006885:Hydroxybutyrates; D007328:Insulin; D007457:Iodine Radioisotopes; D011972:Receptor, Insulin; D020155:3-Hydroxybutyric Acid