title: Expression of I-A alpha k and allelic restriction on A alpha/A beta pairing in transgenic mice.
abstract: Cell surface expression of class 11 major histocompatibility complex encoded (1a) molecules depends on association of the component alpha and beta chain into a stable heterodimer. Studies on cell lines transfected with MHC class II genes have revealed important limitations on the assembly of haplotype-mismatched A alpha:A beta complex. In order to study alpha:beta chain pairing restrictions in vivo a number of lines of transgenic mice carrying the A alpha k gene were generated. The transgene was studied in the context of H-2b, H-2s, H-2q, H-2u, H-2f and H-2v haplotypes. Initial FACS analysis of spleen and peripheral blood cells showed no A alpha kappa expression. Spleen cells stimulated with LPS and analysed by FACS showed A alpha k expression in three different H-2b strains as well as H-2u, but not in others. These data indicate that A alpha k can associate with A beta b and A alpha k, but not with A beta s, A beta q, A beta f, A beta v.
authors.affiliation: Department of Immunology, Mayo Clinic and Medical School, Rochester, MN 55905.
authors.forename: J
authors.initials: J
authors.lastname: Martin
authors.forename: B Y
authors.initials: BY
authors.lastname: Wei
authors.forename: S
authors.initials: S
authors.lastname: Savarirayan
authors.forename: C S
authors.initials: CS
authors.lastname: David
mesh_terms: D000483:Alleles; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D003433:Crosses, Genetic; D015870:Gene Expression; D005802:Genes, MHC Class II; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic
chemical_list: D000949:Histocompatibility Antigens Class II