Of mice and yeast: versatile vectors which permit gene expression in both budding yeast and higher eukaryotic cells.
Gene expression in heterologous species is a powerful tool for the cloning and characterization of genes. Here, we present a family of expression shuttle vectors which work in the budding yeast, Saccharomyces cerevisiae, and also in mammalian cells. The quantity of product expressed by the gene under study can be modulated in yeast by virtue of the control over plasmid copy number and culture conditions.
Camonis
J H
JH
Institut de Génétique et de Microbiologie, U.R.A.C.N.R.S., Université Paris-Sud, Orsay, France.
Cassan
M
M
Rousset
J P
JP
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003062:Codon / Q000235:genetics; D015870:Gene Expression; D005821:Genetic Techniques; D005822:Genetic Vectors; D007739:L Cells / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D003062:Codon; D001616:beta-Galactosidase