Direct interactions between talin and actin.
Talin was purified from chicken gizzard by a modification of the method of L. Molony et al. [J. Biol. Chem.(1987) 262, 7790-7795]. Unlike the talin purified by the previous method, the talin purified by the new method was found to bind to both F- and G-actin: Talin cosedimented with F-actin. On gel filtration of a mixture of talin and G-actin, a complex of talin and action was obtained. Talin stimulated the polymerization rate of G-actin. A major proteolytic fragment of talin that retained the binding ability to F-actin was also identified. These results indicate that talin can bind directly to actin and suggest that talin plays a key role in the organization of actin filaments at the actin-membrane attachment sites in vivo also.
Muguruma
M
M
Department of Animal Science, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Matsumura
S
S
Fukazawa
T
T
D000199:Actins / Q000378:metabolism*; D000818:Animals; D002645:Chickens; D003598:Cytoskeletal Proteins / Q000302:isolation & purification / Q000378:metabolism*; D005895:Gizzard, Avian / Q000378:metabolism; D007700:Kinetics; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000378:metabolism; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000378:metabolism; D011485:Protein Binding; D016608:Talin
D000199:Actins; D003598:Cytoskeletal Proteins; D046911:Macromolecular Substances; D008565:Membrane Proteins; D016608:Talin