Evaluation of dot ELISA technique in the serodiagnosis of schistosomiasis in Egypt.
A simplified dot enzyme linked immunosorbent assay (dot ELISA) was evaluated as a recent diagnostic test for schistosomiasis in Egypt. 160 individuals with early intestinal, urinary and mixed schistosomiasis were subjected to this study. Another 27 sera of patients suffering from other parasitosis were included for the cross activity reaction. The test was compared with 2 common serological tests for schistosomiasis in Egypt, circumoval precipitin test (COPT) and indirect haemagglutination test (IHAT). The dot ELISA was shown to have a sensitivity of 99%, specificity of 98% and the highest diagnostic accuracy 98%. The data indicated that the dot ELISA is an accurate and rapid technique for the detection of antischistosomal antibodies.
el Missiry
A G
AG
Department of Parasitology, Faculty of Medicine, Ain Shams University, Abbassia, Cairo.
el Serougi
A O
AO
Salama
M M
MM
Kamal
A M
AM
D003429:Cross Reactions; D004534:Egypt; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006801:Humans; D011237:Predictive Value of Tests; D012553:Schistosomiasis haematobia / Q000175:diagnosis*; D012555:Schistosomiasis mansoni / Q000175:diagnosis*