Intestinal glycosyl-transferase activities. Nutritional regulation by a chemically-modified protein: methionyl-casein.
In order to estimate the effect of a chemically modified casein, glycosylation processes were studied in rats fed a diet containing this protein. Two groups of rats were fed either a methionyl-casein diet or a normal casein diet. The methionyl-casein was enriched in methionine by covalent linkage of this amino-acid. The nutritional data (growth, protein intake...) were not modified by the diet. The microsomal N-acetylgalactosaminyl- and fucosyl-transferase activities were unaffected by the diet. On the contrary, the soluble fucosyl-transferase activity was enhanced and the activation of fucose transfer in cytosol from methionyl-casein-diet fed rats disappeared after enzyme purification by DEAE-cellulose chromatography. The activation was not explained by changes in some interfering reactions (glycosyl-nucleotide pyrophosphatase, oxidase, protease activities).
Biol
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MC
Department of General and Medical Biochemistry, Lyon-Sud Medical School, INSERM-CNRS U 189, University of Lyon, France.
Martin
A
A
Gaertner
H
H
Puigserver
A
A
Richard
M
M
Louisot
P
P
D000818:Animals; D002364:Caseins / Q000008:administration & dosage / Q000494:pharmacology*; D002848:Chromatography, DEAE-Cellulose; D004247:DNA / Q000032:analysis; D004044:Dietary Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D004789:Enzyme Activation; D005647:Fucosyltransferases / Q000378:metabolism*; D005700:Galactosyltransferases / Q000378:metabolism*; D006031:Glycosylation; D006602:Hexosyltransferases / Q000378:metabolism*; D007421:Intestine, Small / Q000201:enzymology*; D008297:Male; D008715:Methionine / Q000494:pharmacology*; D017350:N-Acetylgalactosaminyltransferases; D051381:Rats; D011919:Rats, Inbred Strains; D000097763:Polypeptide N-acetylgalactosaminyltransferase
D002364:Caseins; D004247:DNA; D004044:Dietary Proteins; D005647:Fucosyltransferases; D005700:Galactosyltransferases; D006602:Hexosyltransferases; D008715:Methionine; D017350:N-Acetylgalactosaminyltransferases; D000097763:Polypeptide N-acetylgalactosaminyltransferase; C020559:Glycoprotein 6-alpha-L-fucosyltransferase