Inhibitors of repair of potentially lethal damage and DNA polymerases also influence the recovery of potentially neoplastic transforming damage in C3H10T-1/2 cells.
The effects of aphidicolin and beta Ara A on radiation sensitivity were evaluated in terms of cell killing, recovery, and neoplastic transformation in the C3H10T-1/2 cell system. When cells were held in plateau phase, recovery of potentially lethal damage (PLD) and potentially transforming damage (PTD) occurred. The addition of beta Ara A resulted in reduced PLD recovery for both the survival and neoplastic transformation end points. The addition of aphidicolin did not affect recovery of PLD or PTD. These data show that the inhibition of polymerase alpha by aphidicolin does not affect recovery of damage leading to cell death or neoplastic transformation. However, the inhibition of both polymerase alpha and beta by beta Ara A resulted in inhibition of recovery of damage leading to both cell death and neoplastic transformation. These data indicated that polymerase beta may be involved in both PLD and PTD recovery.
Raaphorst
G P
GP
Medical Physics Department, Ottawa Regional Cancer Centre, Ontario, Canada.
Azzam
E I
EI
Feeley
M
M
Sargent
M D
MD
D000818:Animals; D016590:Aphidicolin; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects* / Q000528:radiation effects; D004256:DNA Polymerase I / Q000037:antagonists & inhibitors*; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors*; D004260:DNA Repair / Q000187:drug effects* / Q000528:radiation effects; D004224:Diterpenes / Q000494:pharmacology*; D051379:Mice; D008809:Mice, Inbred C3H; D014740:Vidarabine / Q000494:pharmacology*
D004224:Diterpenes; D016590:Aphidicolin; D004256:DNA Polymerase I; D004257:DNA Polymerase II; D014740:Vidarabine