title: [Hepatitis B virus (HBV) replication in patients with various types of chronic hepatopathy].
abstract: We study retrospectively the viral replication state (HBV) of 50 patients with chronic hepatic alterations. The seric DNA-HBV and/or intrahepatic (molecular hybridization), the intrahepatic distribution of HBV antigens (specific monoclonal antibodies labelled with immunoperoxidase), conventional seric HBV markers (commercial enzymoimmunoessay) and the different histopathologic features. We found a correlation between DNA-HBV "in situ" and HBcAg intrahepatic and the seric DNA-HBV production. 81% of the patients with HBsAg (+) had intrahepatic HBcAg and 85% (11/13) of them showed the antigen in their cytoplasms. Patients with HBcAg also had seric and liver DNA-HBV (+). The lack of seric HBsAg did not mean that non-active replication of HBV did not exist because 20% of the patients with HBsAg (-) showed seric and "in situ" DNA-HBV and cytoplasmic HBcAg. The detection of DNA-HBV in endothelial cells and vascular elements in hepatic tissue show that the rate of the HBV host cells is greater.
authors.affiliation: Departamento de Microbiología e Inmunología, Universidad del País Vasco, UPV/EHU, Hospital Civil de Bilbao.
authors.forename: C
authors.initials: C
authors.lastname: Malave
authors.forename: A
authors.initials: A
authors.lastname: Fdez de Aranguiz
authors.forename: L
authors.initials: L
authors.lastname: Sarria
authors.forename: C
authors.initials: C
authors.lastname: Ereño
authors.forename: B
authors.initials: B
authors.lastname: de las Heras
authors.forename: C
authors.initials: C
authors.lastname: Campelo
authors.forename: R
authors.initials: R
authors.lastname: Cisterna
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006505:Hepatitis / Q000097:blood / Q000382:microbiology*; D006510:Hepatitis B Antibodies / Q000032:analysis; D006511:Hepatitis B Antigens / Q000032:analysis; D006515:Hepatitis B virus / Q000502:physiology*; D006801:Humans; D007223:Infant; D008099:Liver / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D014779:Virus Replication
chemical_list: D015415:Biomarkers; D004279:DNA, Viral; D006510:Hepatitis B Antibodies; D006511:Hepatitis B Antigens
vernacular_title: Estudio de la replicación del virus de la hepatitis B (VHB) en pacientes con distintas formas de hepatopatía crónica.