c-fos and c-myc expression in human endothelial cells as a function of different culture conditions.
Cultured human umbilical vein endothelial cells (HEC) could be induced to express c-fos and c-myc mRNA by either serum or ECGS (endothelial cell growth supplement). Neither agonist separately could support HEC proliferation but the combination did. Expression of c-fos and c-myc mRNA in the presence of both serum and ECGS was similar to that observed after each of the two stimuli was introduced separately. c-fos and c-myc expression in cultured HEC, even if related, is not necessarily accompanied by stimulation of cell growth.
Lampugnani
M G
MG
Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
Polentarutti
N
N
Pedenovi
M
M
Mantovani
A
A
Dejana
E
E
Colotta
F
F
D001769:Blood; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D016228:Endothelial Growth Factors; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D015870:Gene Expression; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016760:Proto-Oncogene Proteins c-fos; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger / Q000235:genetics; D014471:Umbilical Veins
D003470:Culture Media; D016228:Endothelial Growth Factors; D006133:Growth Substances; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger