title: Characterization of a chemically synthesized RNA having the sequence of the yeast initiator tRNA(Met).
abstract: A 75-unit long oligoribonucleotide corresponding to the sequence of the Saccharomyces cerevisiae initiator tRNA was synthesized chemically. The crude RNA was purified, and the sequence was verified by RNA sequencing techniques. A particularly useful purification step involved hydrophobic chromatography on BND-cellulose. The purified RNA could be aminoacylated to 28% of a bona fide initiator tRNA(Met) sample and threonylated to 76% of the level observed with native tRNA(fMet) from E. coli.
authors.affiliation: Département de Biochimie, Université de Montréal, Qué, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Bratty
authors.forename: T F
authors.initials: TF
authors.lastname: Wu
authors.forename: K
authors.initials: K
authors.lastname: Nicoghosian
authors.forename: K K
authors.initials: KK
authors.lastname: Ogilvie
authors.forename: J P
authors.initials: JP
authors.lastname: Perreault
authors.forename: G
authors.initials: G
authors.lastname: Keith
authors.forename: R
authors.initials: R
authors.lastname: Cedergren
mesh_terms: D001483:Base Sequence; D006860:Hydrogen Bonding; D008715:Methionine / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012345:RNA, Transfer, Amino Acid-Specific / Q000138:chemical synthesis*; D012358:RNA, Transfer, Met / Q000138:chemical synthesis* / Q000235:genetics / Q000648:ultrastructure; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013912:Threonine / Q000378:metabolism
chemical_list: D012345:RNA, Transfer, Amino Acid-Specific; D012358:RNA, Transfer, Met; D013912:Threonine; D008715:Methionine