title: Mechanical engineering problems in preserving biological objects by temperature lowering.
abstract: Analysis of dangers caused by mechanical refrigerating and liquid nitrogen systems used for low temperature preserving of biological material and safety measures to be adopted. Hazards are caused by moving or protruding parts of the machinery, its hot parts, noise and vibration, work in cold rooms, possible destruction of pressure vessels, refrigerant inflammation or explosion, breathing the refrigerant or its decomposition products, direct contact of the refrigerant with the skin or mucous tissues, depletion of stratospheric ozone or contamination of food-stuffs.
authors.affiliation: Chair of Compressors, Refrigerating Systems and Hydraulic Machinery, Mechanical Engineering Faculty, Technical University of Prague.
authors.forename: Z
authors.initials: Z
authors.lastname: Dvorák
mesh_terms: D004864:Equipment and Supplies; D006280:Health Workforce; D006801:Humans; D009784:Occupational Diseases / Q000209:etiology; D011309:Preservation, Biological; D012034:Refrigeration / Q000009:adverse effects*