title: The blasticidin S resistance gene (bsr) selectable in a single copy state in the Bacillus subtilis chromosome.
abstract: The blasticidin S resistance gene (bsr), originally isolated from Bacillus cereus, was studied in Bacillus subtilis. It was found that a 617 bp fragment including the intact bsr gene and its 5' flanking region could confer BS resistance on B. subtilis when integrated in its chromosome, even in a single copy state. The construction of a bsr gene cassette and its practical application as a novel selection marker for B. subtilis are reported.
authors.affiliation: Mitsubishi Kasei Institute of Life Sciences, Tokyo.
authors.forename: M
authors.initials: M
authors.lastname: Itaya
authors.forename: I
authors.initials: I
authors.lastname: Yamaguchi
authors.forename: K
authors.initials: K
authors.lastname: Kobayashi
authors.forename: T
authors.initials: T
authors.lastname: Endo
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics; D005819:Genetic Markers; D009705:Nucleosides / Q000494:pharmacology; D014169:Transformation, Bacterial
chemical_list: D000935:Antifungal Agents; D005819:Genetic Markers; D009705:Nucleosides; C004500:blasticidin S