title: Adenylate cyclase activity in cyanobacteria: activation by Ca(2+)-calmodulin and a calmodulin-like activity.
abstract: An adenylate cyclase activity was partially characterized in the cyanobacterium Anabaena sp. The enzyme activity is found in soluble cell fractions and shows an apparent molecular weight of about 183,400. This adenylate cyclase is activated by Ca2+ and bovine brain or spinach calmodulin and it is inhibited by EGTA and some phenothiazine derivatives. Furthermore, Anabaena sp. extracts contain a calmodulin-like activity which stimulates bovine brain cyclic AMP phosphodiesterase and the Anabaena adenylate cyclase. EGTA and phenothiazine derivatives block the cyanobacterial modulator effect.
authors.affiliation: Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Buenos Aires, Argentina.
authors.forename: G M
authors.initials: GM
authors.lastname: Bianchini
authors.forename: A C
authors.initials: AC
authors.lastname: Pastini
authors.forename: J P
authors.initials: JP
authors.lastname: Muschietti
authors.forename: M T
authors.initials: MT
authors.lastname: Téllez-Iñón
authors.forename: H E
authors.initials: HE
authors.lastname: Martinetto
authors.forename: H N
authors.initials: HN
authors.lastname: Torres
authors.forename: M M
authors.initials: MM
authors.lastname: Flawiá
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D002118:Calcium / Q000502:physiology; D002147:Calmodulin / Q000502:physiology*; D000458:Cyanobacteria / Q000201:enzymology*; D004789:Enzyme Activation
chemical_list: D002147:Calmodulin; D000262:Adenylyl Cyclases; D002118:Calcium