title: Immunohistochemical demonstration of a thiamine diphosphate-binding protein in the brain of the adult rat.
abstract: We recently purified a new thiamine diphosphate-binding protein (ThDP-BP) prepared from rat liver, and found immunoreactivity for ThDP-BP in neurons and other neuronal tissues. In this study, we examined the distribution of ThDP-BP in the rat neurons by the avidin-biotin complex technique. Strong immunoreactivity for ThDP-BP was found in the cerebral cortex, hippocampus, ependymal cells, nucleus III, nucleus V, and medial vestibular nucleus. Moderate immunoreactivity was noted in the olfactorius nucleus anterior and weak activity in the nucleus ruber. ThDP-BP may be useful in clarifying the pathogenesis of Wernicke encephalopathy and Leigh's disease.
authors.affiliation: Department of Geriatric Medicine, Faculty of Medicine, Kyoto University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yoshioka
authors.forename: K
authors.initials: K
authors.lastname: Nishino
authors.forename: G
authors.initials: G
authors.lastname: Ohshio
authors.forename: T
authors.initials: T
authors.lastname: Kimura
authors.forename: T
authors.initials: T
authors.lastname: Sugiyama
authors.forename: T
authors.initials: T
authors.lastname: Kita
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D007150:Immunohistochemistry; D009474:Neurons / Q000378:metabolism; D051381:Rats; D013835:Thiamine Pyrophosphate / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D002352:Carrier Proteins; D013835:Thiamine Pyrophosphate