Effect of clomiphene citrate on oestrogen secretion by human granulosa cells in culture.
The effect of clomiphene citrate (CC) and 17 beta-oestradiol (E2) on oestrogen secretion was studied in human preovulatory granulosa cells in culture. CC stimulated E2 secretion at 1 and 10 ng/ml (respectively, +36 +/- 14% and +33 +/- 5%) in basal conditions but had no effect on FSH-stimulated E2 secretion. On the other hand, E2 had no effect at any of the tested concentrations (0.1 ng/ml to 1000 ng/ml) on its own production. Since the plasma level of CC obtained in clinical use ranges from 1 to 10 ng/ml, this finding could explain the higher plasma oestrogen levels obtained with CC as compared to human menopausal gonadotrophin.
Bussenot
I
I
Laboratoire de Fécondation In Vitro, INSERM CJF 89/08, Toulouse, France.
Parinaud
J
J
Clamagirand
C
C
Vieitez
G
G
Pontonnier
G
G
D000735:Androstenedione / Q000378:metabolism; D002478:Cells, Cultured; D002996:Clomiphene / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000378:metabolism* / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans
D002996:Clomiphene; D000735:Androstenedione; D004958:Estradiol; D005640:Follicle Stimulating Hormone