Effect of plasma glucose concentration on cerebral metabolism during partial ischemia in neonatal piglets.
We used neonatal piglets to determine the influence of plasma glucose concentration on cerebral energy metabolism during and immediately after partial ischemia. We assessed cerebral metabolism using in vivo phosphorus-31 magnetic resonance spectroscopy. Arterial plasma glucose concentration was increased in four piglets by systemic infusions of dextrose in water for comparison with infusions of saline in four controls or decreased in eight piglets by fasting for 24-48 hours for comparison with four fed piglets. Plasma glucose concentration showed a significant linear correlation with intracellular pH (r = -0.7, p less than 0.05). Piglets that developed hypoglycemia during partial ischemia had a smaller reduction in intracellular pH and a larger increase in inorganic phosphate content than piglets that were normoglycemic or hyperglycemic during ischemia. Similar differences persisted during the first 5 minutes of postischemic reperfusion. Subsequently, the cerebral concentrations of phosphorylated compounds returned to normal in all piglets. Our results demonstrate that 1) arterial plasma glucose concentration influences cerebral energy metabolism and intracellular pH during ischemia, 2) neonatal piglets can develop profound brain acidosis, and 3) brain acidosis during ischemia does not influence the restoration of cerebral phosphorylated compounds to control levels during the first 90 minutes after ischemia.
Laptook
A R
AR
Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas 75235.
Corbett
R J
RJ
Nunnally
R L
RL
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001786:Blood Glucose / Q000032:analysis*; D001921:Brain / Q000378:metabolism*; D002545:Brain Ischemia / Q000097:blood / Q000378:metabolism*; D002245:Carbon Dioxide / Q000097:blood; D005215:Fasting; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000378:metabolism; D009994:Osmolar Concentration; D010313:Partial Pressure; D010725:Phosphocreatine / Q000378:metabolism; D013552:Swine
D001786:Blood Glucose; D010725:Phosphocreatine; D002245:Carbon Dioxide; D000255:Adenosine Triphosphate