Isolation of an enzyme catalysing nitrosamine formation in Pseudomonas aeruginosa and Neisseria mucosae.
An enzyme catalysing nitrosamine formation was isolated and purified from two denitrifying microorganisms, Pseudomonas aeruginosa and Neisseria mucosae. The soluble enzyme has a molecular weight of 66 as determined by gel filtration and SDS-polyacrylamide gel electrophoresis and a pH optimum for P. aeruginosa of 7.25. A number of microorganisms isolated from human infections have previously been found to possess nitrosating enzymes.
Calmels
S
S
International Agency for Research on Cancer, Lyon, France.
Dalla Venezia
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N
Bartsch
H
H
D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008970:Molecular Weight; D009343:Neisseria / Q000201:enzymology*; D009602:Nitrosamines / Q000378:metabolism*; D010088:Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000201:enzymology*
D009602:Nitrosamines; D010088:Oxidoreductases; C065724:nitrosating enzyme