[Model of intraluminal perfusion of the guinea pig ileum in vitro in the study of the antidiarrheal properties of the guava (Psidium guajava)].
An experimental in vitro model was developed for the study of plant extracts reported by traditional medicines in the treatment of diarrhea. The guinea-pig isolated ileum is perfused with the plant extract using an intraluminal approach. The peristaltic reflex is induced by electrical stimulation while the plant extract is perfused. The spasmolytic effects of Psidium guajava leaf methanol, hexane and water extracts were demonstrated suggesting the existence of two different types of active components. The results obtained allow to propose this in vitro method as a useful model to reproduce some of the characteristics of the oral way of administration of plant extracts.
Lozoya
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X
Unidad de Investigación en Medicina Tradicional y Desarrollo de Medicamentos, IMSS.
Becerril
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Martínez
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M
D000818:Animals; D000930:Antidiarrheals / Q000302:isolation & purification / Q000494:pharmacology*; D005260:Female; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects*; D008297:Male; D008954:Models, Biological; D010276:Parasympatholytics / Q000302:isolation & purification / Q000494:pharmacology; D010477:Perfusion; D010528:Peristalsis / Q000187:drug effects*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D010946:Plants, Medicinal / Q000032:analysis; D012995:Solubility
D000930:Antidiarrheals; D010276:Parasympatholytics; D010936:Plant Extracts
Modelo de perfusión intraluminal del ileon del cobayo in vitro en el estudio de las propiedades antidiarréicas de la guayaba (Psidium guajava).