Alkaline phosphatase from adult rat femur.
Four alkaline phosphatase forms from adult rat femur were distinguished on polyacrylamide gel electrophoresis: two soluble forms of Mr 165,000 and 110,000 in the water extract, and three membrane-bound forms of Mr 130,000, 110,000 and 100,000 extractable with deoxycholate. Alkaline phosphatase after SDS-treatment disintegrated into three kinds of monomers: of Mr 80,000, 65,000 and 50,000. The soluble fraction (extract I) contained subunits of Mr 80,000 and 55,000--whereas the pellet fraction (extract II), subunits of Mr 65,000 and 50,000. Since for native forms only three types of subunits were found it seems that, apart from homodimers, there are also some heterodimers composed of the Mr 65,000 and 50,000 subunits forming the native enzyme of Mr 110,000-115,000. Two denatured monomers: of Mr 80,000 and 50,000 may form two native homodimeric forms of Mr 165,000 and 100,000 while in the pellet two monomers: of Mr 65,000 and 50,000 may correspond to three native alkaline phosphatase forms: of Mr 130,000, 110,000-115,000 and 100,000. Probably the Mr 110,000-115,000 form is a heterodimer composed of subunits of Mr 65,000 and 50,000.
Tardivel
S
S
Métabolism Minéral des Mammiféres, EPHE, Faculté de Pharmacie, Châtenay-Malabry, France.
Dupuis
Y
Y
Fournier
P
P
Zelazowska
E
E
Graboń
W
W
Porembska
Z
Z
D000469:Alkaline Phosphatase / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D001842:Bone and Bones / Q000201:enzymology*; D002113:Calcification, Physiologic / Q000502:physiology; D005591:Chemical Fractionation; D004591:Electrophoresis, Polyacrylamide Gel; D005269:Femur; D007124:Immunoenzyme Techniques; D008297:Male; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D012710:Serum Albumin, Bovine
D012710:Serum Albumin, Bovine; D000469:Alkaline Phosphatase