title: A root acyl carrier protein-II from spinach is also expressed in leaves and seeds.
abstract: During the synthesis of fatty acids and their utilization in plastids, fatty acyl moieties are linked to acyl carrier protein (ACP). In contrast to previously cloned organ-specific ACP isoforms, we have now isolated a cDNA clone for a potentially constitutive ACP isoform from a spinach root library. Identity between the amino acid sequence encoded by this cDNA and N-terminal sequence data for ACP-II protein from spinach leaf indicates that the root cDNA encodes ACP-II. The deduced amino acid sequence for ACP-II shows 62% identity with spinach leaf ACP-I. Southern analysis suggests that multiple ACP genes or pseudogenes occur in the spinach genome. High-stringency northern blot analysis and RNase protection studies confirm that, within the region encoding the mature ACP-II, the cloned ACP sequence is expressed in leaves and seeds as well as in roots. Quantitative RNase protection data indicate that the ratio of ACP-I and ACP-II mRNA sequences in leaf is similar to the ratio of the two proteins.
authors.affiliation: Department of Botany and Plant Pathology, Michigan State University, East Lansing 48824.
authors.forename: K M
authors.initials: KM
authors.lastname: Schmid
authors.forename: J B
authors.initials: JB
authors.lastname: Ohlrogge
mesh_terms: D000213:Acyl Carrier Protein / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA; D015870:Gene Expression; D017343:Genes, Plant; D008040:Genetic Linkage; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000033:anatomy & histology / Q000235:genetics; D015347:RNA Probes; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D015183:Restriction Mapping; D012260:Ribonucleases; D012639:Seeds; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000213:Acyl Carrier Protein; D010940:Plant Proteins; D015347:RNA Probes; D012333:RNA, Messenger; C070639:ACP-II protein, spinach; D004247:DNA; D012260:Ribonucleases