Autocrine growth by two cytokines, interleukin-6 and tumor necrosis factor alpha, in the myeloma cell line KHM-1A.
The effects of tumor necrosis factor alpha (TNF-alpha) and interleukin-6 (IL-6) on the cell growth of the myeloma cell line KHM-1A were studied. TNF-alpha strongly induced KHM-1A proliferation, while IL-6 weakly enhanced growth only at low cell densities. When TNF-alpha was combined with IL-6, TNF-alpha-induced growth enhancement was reduced. According to Northern blotting analysis, the m-RNA of both TNF-alpha and IL-6 were detected in KHM-1A. Moreover, monoclonal antibody capable of neutralizing the cytotoxic activity of TNF-alpha inhibited the proliferation of this cell line. These findings suggest that this cell line operates under an autocrine growth mechanism with respect to these two cytokines, IL-6 and TNF-alpha.
Hata
H
H
Second Department of Internal Medicine, Kumamoto University Medical School, Japan.
Matsuzaki
H
H
Takatsuki
K
K
D000911:Antibodies, Monoclonal; D002455:Cell Division / Q000276:immunology; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D009101:Multiple Myeloma / Q000276:immunology* / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D000911:Antibodies, Monoclonal; D015850:Interleukin-6; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D014409:Tumor Necrosis Factor-alpha