[IL-2 production by peripheral blood mononuclear cells after stimulation with pokeweed mitogen].
Peripheral blood mononuclear cells were cultured for 72 hours in presence of phytohaemagglutinin (PHA), phytohaemagglutinin/phorbolmyristate acetate (PHA/PMA), pokeweed mitogen (PWM) and in absence of these stimulators. The IL-2 concentrations of cultural supernatants were determined by an IL-2 dependent cell line. There was no IL-2 determined in absence of any mitogenic stimulators. Induction of IL-2 by PWM does not require the presence of PMA in contrast to stimulation by PHA/PMA, where PMA was found to be necessary for stimulation of production of IL-2. PHA/PMA induced IL-2 release shows only a weak maximum at 24 hours, whereas PWM induced IL-2 release was found to have a clear maximum at 48 hours. Stimulation by PWM enables comparative examination of DNA synthesis (lymphocyte transformation test) and IL-2 production in the same cell system using only one mitogen.
Reinhold
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Forschungsabteilung Experimentelle Immunologie, Medizinischen Akademie Magdeburg.
Ansorge
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D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000187:drug effects; D011043:Pokeweed Mitogens / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007376:Interleukin-2; D011043:Pokeweed Mitogens; D013755:Tetradecanoylphorbol Acetate
IL-2-Produktion mononukleärer Zellen des peripheren Blutes nach Stimulation mit Pokeweed-Mitogen.