Increased resolution of lipopolysaccharides and lipooligosaccharides utilizing tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
We utilized the recently described tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (TSDS-PAGE) system to study the lipooligosaccharides (LOS) and lipopolysaccharides (LPS) of gram negative bacteria. TSDS-PAGE resulted in a high degree of resolution of LOS and LPS in the 'mini-gel' format. TSDS-PAGE resulted in the LOS and LPS migrating as a function of their Mr during electrophoresis and allowed estimation of Mr from a protein standard. Several species of LOS were analyzed. The newly described procedure allowed a more rapid and accurate analysis of LOS and the core region of LPS.
Lesse
A J
AJ
Department of Medicine, State University of New York, Buffalo.
Campagnari
A A
AA
Bittner
W E
WE
Apicella
M A
MA
D004591:Electrophoresis, Polyacrylamide Gel; D005998:Glycine / Q000031:analogs & derivatives / Q000494:pharmacology; D006193:Haemophilus influenzae / Q000032:analysis; D008070:Lipopolysaccharides / Q000032:analysis*; D008970:Molecular Weight; D009344:Neisseria gonorrhoeae / Q000032:analysis; D012475:Salmonella / Q000032:analysis
D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides; D005998:Glycine; C100184:tricine