The gamma subunit of brain G-proteins is methyl esterified at a C-terminal cysteine.
The gamma polypeptide of brain G-proteins is carboxyl methylated when the purified beta gamma subunit complex is reconstituted with S-adenosyl-[3H-methyl]-L-methionine and a methyltransferase present in detergent-stripped brain membranes. By chromatographic analysis of the 3H-amino acid generated by exhaustive proteolysis and performic acid oxidation of the 3H-methylated beta gamma complex, we show that this modification occurs on the alpha-carboxyl group of a C-terminal cysteine residue. Our result suggests that brain G-protein may undergo multiple covalent modification steps, including proteolytic removal of the three terminal amino acids from the predicted common C-terminal Cys-Xaa-Xaa-Xaa sequence, and the methyl esterification of the resulting terminal cysteine residue. This modification is likely to be associated with lipidation at the sulfhydryl group of the same cysteine, which would explain the tight membrane binding property of the brain beta gamma complex.
Fung
B K
BK
Jules Stein Eye Institute, University of California, Los Angeles 90024.
Yamane
H K
HK
Ota
I M
IM
Clarke
S
S
D000818:Animals; D001923:Brain Chemistry; D002264:Carboxylic Acids / Q000032:analysis*; D002417:Cattle; D002462:Cell Membrane / Q000502:physiology; D003545:Cysteine / Q000032:analysis*; D004952:Esters / Q000032:analysis*; D019204:GTP-Binding Proteins / Q000302:isolation & purification* / Q000502:physiology; D006868:Hydrolysis; D008563:Membrane Lipids / Q000502:physiology; D008745:Methylation; D011485:Protein Binding; D012436:S-Adenosylmethionine
D002264:Carboxylic Acids; D004952:Esters; D008563:Membrane Lipids; D012436:S-Adenosylmethionine; D019204:GTP-Binding Proteins; D003545:Cysteine