Effects of ganglioside (Cronassial) treatment on MHC Ia antigen expression and allograft survival of pancreatic islets in diabetic rats.
Streptozotocin-diabetic BdII rats were treated daily with 20 mg/kg body weight gangliosides for ten days beginning two days before transplantation. This treatment did not prolong allograft survival of untreated Lewis islets. Culture treatment of isolated Lewis islets with gangliosides (100 micrograms/ml in RPMI 1640) for one day resulted in a significant reduction of MHC Ia antigen positive cells but not of class I antigens within the islets. Transplantation of the ganglioside pretreated islets into non-immunosuppressed BdII recipients prolonged allograft survival to 12 days only in one of five animals.
Bretzel
R G
RG
3rd Medical Department, Justus-Liebig-University of Giessen, FRG.
Flesch
B K
BK
Willig
J
J
Woehrle
M
M
Federlin
K
K
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000276:immunology* / Q000601:surgery; D005732:Gangliosides / Q000494:pharmacology*; D006085:Graft Survival / Q000187:drug effects*; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D007515:Islets of Langerhans / Q000187:drug effects / Q000276:immunology*; D016381:Islets of Langerhans Transplantation; D051381:Rats; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains; D014184:Transplantation, Homologous
D005732:Gangliosides; D000949:Histocompatibility Antigens Class II; C037470:cronassial