Polyclonal antibody-based enzyme-linked immunosorbent assay of alpha 1-acid glycoprotein.
This is a noncompetitive enzyme-linked immunosorbent assay for measuring low concentrations (2 to 100 micrograms/L) of human alpha 1-acid glycoprotein (AGP; orosomucoid). The method is based on a simple "sandwich" technique involving polyclonal rabbit antisera against AGP. Mean within-run and total (between-run) CVs were 6.2% and 9.7%, respectively. Analytical recovery, tested in various biological fluids, averaged 101%. The technique has been successfully applied to diluted biological fluids such as bronchoalveolar lavage, cerebrospinal and amniotic fluids, and hepatocyte-culture supernates. Because of its analytical validity and the commercial availability of the reagents, this assay is suitable for large-scale determinations of AGP concentrations in those biological fluids in which its concentration is relatively low.
Tissot
B
B
Laboratoire de Biochimie A, Hôpital Bichat, Paris, France.
Seta
N
N
Durand
G
G
Feger
J
J
D000653:Amniotic Fluid / Q000032:analysis; D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D001992:Bronchoalveolar Lavage Fluid / Q000032:analysis; D003470:Culture Media / Q000032:analysis; D006801:Humans; D005779:Immunodiffusion; D007202:Indicators and Reagents; D008832:Microchemistry; D009961:Orosomucoid / Q000032:analysis* / Q000134:cerebrospinal fluid / Q000276:immunology
D000906:Antibodies; D003470:Culture Media; D007202:Indicators and Reagents; D009961:Orosomucoid