Levuglandin E2 inhibits mitosis and microtubule assembly.
Levuglandin E2 (LGE2) is a gamma-keto aldehyde produced by rearrangement of the prostaglandin endoperoxide PGH2 under the aqueous conditions of its biosynthesis. We show that exogenous LGE2 enters cells and efficiently inhibits the first synchronous cell division of fertilized sea urchin eggs. We attribute this inhibition to covalent modification of tubulin and thereby to inhibition of microtubule assembly.
Murthi
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KK
Department of Chemistry, Case Western Reserve University, Cleveland, Ohio 44106-2699.
Salomon
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RG
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D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D001921:Brain / Q000648:ultrastructure; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D015232:Dinoprostone / Q000494:pharmacology; D007700:Kinetics; D008868:Microtubule Proteins / Q000302:isolation & purification / Q000378:metabolism; D008870:Microtubules / Q000187:drug effects* / Q000648:ultrastructure; D008938:Mitosis / Q000187:drug effects*; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects; D011458:Prostaglandins E / Q000378:metabolism / Q000494:pharmacology*; D011485:Protein Binding; D012617:Sea Urchins
D001095:Arachidonic Acids; D008868:Microtubule Proteins; D011458:Prostaglandins E; D016718:Arachidonic Acid; C047684:levuglandin E2; D015232:Dinoprostone