Rapid phosphorylation of cellular proteins during differentiation of neuroblastoma cells induced by recombinant human interferon-gamma.
Cells of two human neuroblastoma lines, GOTO and KP-N-RT-LN, differentiated in response to treatment with 1,000 IU/ml of recombinant human interferon-gamma (rIFN-gamma), but did not respond to rIFN-alpha 2 or IFN-beta. Treatment with rIFN-gamma rapidly increased the phosphorylation of several cell proteins; in particular, there was enhanced phosphorylation of a 61-kD protein within 1 min after treatment. This enhancement was not observed in variant sublines of GOTO cells, which did not differentiate when treated with rIFN-gamma. These findings suggest that when neuroblastoma cells are induced to differentiate by treatment with rIFN-gamma, phosphorylation of the 61-kD protein plays an important role.
Higuchi
T
T
Department of Microbiology, Kyoto Prefectural University of Medicine, Japan.
Tanaka
A
A
Watanabe
H
H
Chisaka
T
T
Imanishi
J
J
D002454:Cell Differentiation / Q000187:drug effects; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D009363:Neoplasm Proteins / Q000302:isolation & purification / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism*; D010750:Phosphoproteins / Q000302:isolation & purification; D010766:Phosphorylation / Q000187:drug effects; D012330:RNA, Double-Stranded / Q000235:genetics; D011994:Recombinant Proteins; D013997:Time Factors; D014407:Tumor Cells, Cultured
D009363:Neoplasm Proteins; D010750:Phosphoproteins; D012330:RNA, Double-Stranded; D011994:Recombinant Proteins; D007371:Interferon-gamma