Bacterial adherence to extended wear soft contact lenses.
The authors studied the adherence of Pseudomonas aeruginosa and Staphylococcus aureus to extended wear soft contact lenses (EWSCLs) with and without focal deposits using both a radiolabeling technique and electron microscopy. P. aeruginosa showed significant adherence to contact lenses in vitro. In contrast, S. aureus failed to show significant adherence to contact lenses in vitro (i.e., the radioactive uptake was not significantly above background). The extent of adherence of Pseudomonas was proportional to the number of focal deposits on the lenses. Results of electron microscopic examination showed the bacteria to be adherent primarily to large focal deposits (greater than or equal to 150 microns). There was no pseudomonal adherence to the small focal deposits (less than or equal to 50 microns) and little adherence to the areas in between the focal deposits. The authors hypothesize that worn lenses, especially those with large focal deposits, serve as a vehicle for the transport of P. aeruginosa to the cornea. This hypothesis could be a partial explanation for the high incidence of keratitis caused by P. aeruginosa in EWSCL patients.
Aswad
M I
MI
Department of Ophthalmology, New England Medical Center, Boston, MA 02111.
John
T
T
Barza
M
M
Kenyon
K
K
Baum
J
J
D001422:Bacterial Adhesion; D015169:Colony Count, Microbial; D003262:Contact Lenses, Extended-Wear; D006801:Humans; D008855:Microscopy, Electron, Scanning; D011550:Pseudomonas aeruginosa / Q000254:growth & development* / Q000648:ultrastructure; D013211:Staphylococcus aureus / Q000254:growth & development* / Q000648:ultrastructure; D013936:Thymidine; D014316:Tritium
D014316:Tritium; D013936:Thymidine