title: Nucleotide sequence of the Pseudomonas aeruginosa phoB gene, the regulatory gene for the phosphate regulon.
abstract: The nucleotide sequence of Pseudomonas aeruginosa phoB was determined. The sequence data suggest that the PhoB polypeptide consists of 229 amino acid residues and has a predicted molecular weight of 25,708. In the regulatory region of the gene, a very well conserved phosphate box was found. The sequence data also predicted the presence of an open reading frame downstream of phoB, which could be phoR. The deduced amino acid sequence of phoB was significantly homologous to that of the Escherichia coli phoB gene product and to those of several known procaryotic transcriptional regulators such as PhoP, OmpR, VirG, Dye, NtrC, and AlgR.
authors.affiliation: Laboratoire de Chimie Bactérienne, C.N.R.S. Marseille, France.
authors.forename: J
authors.initials: J
authors.lastname: Anba
authors.forename: M
authors.initials: M
authors.lastname: Bidaud
authors.forename: M L
authors.initials: ML
authors.lastname: Vasil
authors.forename: A
authors.initials: A
authors.lastname: Lazdunski
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D005809:Genes, Regulator; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009154:Mutation; D009876:Operon; D010710:Phosphates / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D010710:Phosphates