title: Bacillus amyloliquefaciens alpha-amylase signal sequence fused in frame with human proinsulin is properly processed by Bacillus subtilis cells.
abstract: The plasmid pBINS1, containing the promoter, SD and leader peptide sequences of Bacillus amyloliquefaciens alpha-amylase gene and 267 bp long sequence coding for human proinsulin directs the efficient synthesis of hybrid preproinsulin, as well as quantitative secretion of proinsulin outside of protease-deficient Bacillus subtilis AJ73 cells. The recombinant proinsulin has been isolated from the culture medium and its N-terminal sequence shown to be identical with that of natural human prohormone.
authors.affiliation: Institute of Molecular Genetics, USSR Academy of Sciences, Moscow.
authors.forename: A A
authors.initials: AA
authors.lastname: Novikov
authors.forename: S I
authors.initials: SI
authors.lastname: Borukhov
authors.lastname: Strongin AYa
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D001412:Bacillus subtilis / Q000235:genetics*; D003001:Cloning, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D011384:Proinsulin / Q000096:biosynthesis / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D000516:alpha-Amylases / Q000235:genetics*
chemical_list: D011994:Recombinant Proteins; D011384:Proinsulin; D000516:alpha-Amylases