Estrogen induced expression of the C-jun proto-oncogene in the immature and mature rat uterus.
The expression of the proto-oncogene c-jun in response to estradiol treatment in immature and mature rat uterine tissue was measured using a cDNA encoding the mouse c-jun proto-oncogene. This probe hybridized to a major RNA band of 2.7 kb and a minor 3.2 kb band. In Northern blots of total RNA from both immature and mature rat uteri, estradiol treatment resulted in at least a 3 fold increase in expression of the 2.7 kb band over control levels by 3 hr post injection. By 12 hr post injection, expression of c-jun mRNA had returned to control levels. A strong induction (greater than 5 fold) of c-jun mRNA expression was also observed in stroma-myometrial tissue isolated from mature rats approximately 3 hours after treatment with estradiol. The similar kinetics of induction of c-fos and c-jun emphasizes the functional significance of the fos/jun heterodimer in control of uterine cell proliferation.
Webb
D K
DK
Department of Anatomy and Cell Biology, University of Cincinnati, College of Medicine, OH 45267.
Moulton
B C
BC
Khan
S A
SA
D000818:Animals; D001345:Autoradiography; D015152:Blotting, Northern; D004268:DNA-Binding Proteins / Q000235:genetics*; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D007700:Kinetics; D009215:Myometrium / Q000187:drug effects / Q000378:metabolism*; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D014157:Transcription Factors / Q000235:genetics*; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
D004268:DNA-Binding Proteins; D004967:Estrogens; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D014157:Transcription Factors