Positive and negative effects of diagnostic intensities of ultrasound on erythrocyte blood group markers.
Human erythrocytes were exposed in vitro to diagnostic intensities of ultrasound. Various antigenic tests were conducted to see if the different blood group markers had been changed. No change was observed following sonication for Ss, M, K1, ABO, and the rhesus factors. However a marked reduction was found for the N antigen when assayed using the lectin isolated from Vicia graminea. Several experiments were performed to ensure that this effect was not an artefact. If the cells were not resuspended in fresh medium immediately following sonicating then the magnitude of the effect rapidly decreased. We were unable to detect free N antigen in the supernatants from the sonicated cell suspensions. We were also unable to demonstrate changes in the level of the N antigen following sonication if we used anti-N sera from rabbits. A surprising observation was that different batches of the lectin preparation from the same manufacturer could eliminate the effect.
Rosenfeld
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Department of Applied Biophysics, Martin Luther University Halle-Wittenberg, GDR.
Romanowski
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Williams
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AR
D000017:ABO Blood-Group System / Q000276:immunology; D000372:Agglutination Tests; D000954:Antigens, Surface / Q000502:physiology; D001789:Blood Group Antigens / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D007519:Isoantigens / Q000502:physiology*; D007626:Kell Blood-Group System / Q000276:immunology; D008951:MNSs Blood-Group System / Q000276:immunology; D012204:Rh-Hr Blood-Group System / Q000276:immunology; D014465:Ultrasonics
D000017:ABO Blood-Group System; D000954:Antigens, Surface; D001789:Blood Group Antigens; D007519:Isoantigens; D007626:Kell Blood-Group System; D008951:MNSs Blood-Group System; D012204:Rh-Hr Blood-Group System