A critical review of permeabilized cell systems for studying mammalian DNA repair.
Permeabilized cell systems have proven valuable for studies of the enzymology of mammalian DNA repair and this review will summarize and contrast the different systems used to this end. Results from permeable cell studies will be reviewed which pertain to 3 questions of DNA repair: the role(s) of ATP, DNA polymerase enzymology, and the isolation of repair factors by in vitro correction of repair-defective cells.
Keeney
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Division of Biochemistry and Molecular Biology, University of California, Berkeley 94720.
Linn
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D000255:Adenosine Triphosphate / Q000502:physiology; D000818:Animals; D016590:Aphidicolin; D002463:Cell Membrane Permeability; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors; D004258:DNA Polymerase III; D004260:DNA Repair; D004259:DNA-Directed DNA Polymerase / Q000494:pharmacology / Q000502:physiology; D054306:Dideoxynucleotides; D004224:Diterpenes / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D008322:Mammals; D013942:Thymine Nucleotides / Q000494:pharmacology
D054306:Dideoxynucleotides; D004224:Diterpenes; D013942:Thymine Nucleotides; D016590:Aphidicolin; D000255:Adenosine Triphosphate; D004257:DNA Polymerase II; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase; C017216:2',3'-dideoxythymidine triphosphate