Cycloheximide enhances factor binding to the native 40S ribosomal subunit of Microsporum canis.
Native and derived ribosomal particles from the mycelial cells of Microsporum canis grown in the presence and absence of cycloheximide were compared by CsCl equilibrium density gradient centrifugation. Since the buoyant densities of ribonucleoprotein complexes are dependent on the protein-RNA ratio, they reflect the composition of these particles. The native monosomes from cells grown in the presence and absence of cycloheximide had a buoyant density of 1.585 g/cc. The native 60S subunits showed a density of 1.540 g/cc from cells grown in both presence and absence of cycloheximide, while the derived subunits showed a density of 1.610 g/cc. The derived 40S subunits had a density of 1.550 g/cc while the native 40S showed a major species of density 1.535 g/cc with three other minor species ranging in densities from 1.450-1.390 g/cc. The mycelia grown in the presence of cycloheximide showed an increased proportion of native 40S subunits in the density range of 1.450-1.390 g/cc, indicating that the drug enhances factor binding to native ribosomal subunits in M. canis.
Mandiyan
V
V
Microbiology and Cell Biology Laboratory, Indian Institute of Science, Bangalore.
D002499:Centrifugation, Density Gradient; D003513:Cycloheximide / Q000494:pharmacology*; D005656:Fungal Proteins / Q000378:metabolism; D008864:Microsporum / Q000187:drug effects* / Q000378:metabolism; D010448:Peptide Initiation Factors / Q000378:metabolism; D011485:Protein Binding; D012270:Ribosomes / Q000187:drug effects* / Q000378:metabolism
D005656:Fungal Proteins; D010448:Peptide Initiation Factors; D003513:Cycloheximide