[Studies on dynamics of antibodies in sera from rabbits immunized with homologous and heterologous adult filarial antigens].
The indirect immunofluorescent antibody test (IFAT) and immunoenzymatic staining technique (IEST) with frozen sections of Brugia malayi and Setaria cervi adult worms as antigens were used to detect IgG levels in rabbits immunized with three different filarial antigens. The results showed the dynamics of the specific IgG antibody in the course of immunization. The peaks (GMRT 76.11-861.08) of IgG levels in immunized rabbits appeared at the 4th week after primary immunization, which returned to (4.00-32.0 up to the 8th week after primary immunization and rose to (20.11-181.02) at the 1st week after second immunization. It is suggested that IFAT and IEST, especially the latter, can be applied for serodiagnosis of filarial infection and both antigens are considered to be of similar diagnostic effect.
Wu
H
H
Department of Parasitology, Suzhou Medical College.
Li
Y H
YH
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D000947:Antigens, Helminth / Q000276:immunology*; D002009:Brugia / Q000276:immunology*; D004183:Dirofilaria immitis / Q000276:immunology*; D007074:Immunoglobulin G / Q000032:analysis*; D011817:Rabbits
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G