Effect of thyroid hormone on hepatic cholesterol 7 alpha hydroxylase, LDL receptor, HMG-CoA reductase, farnesyl pyrophosphate synthetase and apolipoprotein A-I mRNA levels in hypophysectomized rats.
The effects of thyroid hormone on cholesterol 7 alpha hydroxylase, LDL receptor, HMG-CoA reductase, apo A-I and farnesyl pyrophosphate synthetase hepatic mRNA levels were investigated in hypophysectomized rats. Of these mRNAs cholesterol 7 alpha hydroxylase responded the most rapidly and required the lowest dose of T3. Maximal mRNA levels were reached one hr after T3 administration and required 10 micrograms/100g of body weight. These results suggest that the hypocholesterolemic effect of thyroid hormone may be mediated by a primary effect on cholesterol 7 alpha hydroxylase gene expression.
Ness
G C
GC
Department of Biochemistry and Molecular Biology, College of Medicine, University of South Florida, Tampa 33612.
Pendleton
L C
LC
Li
Y C
YC
Chiang
J Y
JY
D000818:Animals; D000924:Anticholesteremic Agents / Q000494:pharmacology; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D002790:Cholesterol 7-alpha-Hydroxylase / Q000235:genetics*; D015342:DNA Probes; D004122:Dimethylallyltranstransferase / Q000235:genetics*; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics*; D006937:Hypercholesterolemia / Q000188:drug therapy; D007016:Hypophysectomy; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011973:Receptors, LDL / Q000235:genetics*; D014284:Triiodothyronine / Q000494:pharmacology
D000924:Anticholesteremic Agents; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D015342:DNA Probes; D012333:RNA, Messenger; D011973:Receptors, LDL; D014284:Triiodothyronine; D006903:Hydroxymethylglutaryl CoA Reductases; D002790:Cholesterol 7-alpha-Hydroxylase; D004122:Dimethylallyltranstransferase