Beta-lactam antibiotic biosynthetic genes have been conserved in clusters in prokaryotes and eukaryotes.
A cosmid clone containing closely linked beta-lactam antibiotic biosynthetic genes was isolated from a gene library of Flavobacterium sp. SC 12,154. The location within the cluster of the DNA thought to contain the gene for delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase (ACVS), the first step in the beta-lactam antibiotic biosynthetic pathway, was identified by a novel method. This DNA facilitated the isolation, by cross-hybridization, of the corresponding DNA from Streptomyces clavuligerus ATCC 27064, Penicillium chrysogenum Oli13 and Aspergillus nidulans R153. Evidence was obtained which confirmed that the cross-hybridizing sequences contained the ACVS gene. In each case the ACVS gene was found to be closely linked to other beta-lactam biosynthetic genes and constituted part of a gene cluster.
Smith
D J
DJ
Department of Microbiology, University of Bristol, UK.
Burnham
M K
MK
Bull
J H
JH
Hodgson
J E
JE
Ward
J M
JM
Browne
P
P
Brown
J
J
Barton
B
B
Earl
A J
AJ
Turner
G
G
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001233:Aspergillus nidulans / Q000235:genetics*; D003001:Cloning, Molecular; D003360:Cosmids; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D005417:Flavobacterium / Q000235:genetics*; D015723:Gene Library; D005798:Genes, Bacterial; D005800:Genes, Fungal; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D010407:Penicillium / Q000235:genetics*; D010408:Penicillium chrysogenum / Q000235:genetics*; D010453:Peptide Synthases / Q000235:genetics; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013302:Streptomyces / Q000235:genetics*; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004271:DNA, Fungal; D047090:beta-Lactams; D010453:Peptide Synthases; C095368:alpha-aminoadipyl-cysteinyl-valine synthetase