title: [Developement and quality control of a radioimmunoassay for measurement of endogenous digoxin].
abstract: It has been suggested that sodium renal excretion is regulated, at least partially, by a factor with natriuretic properties called digoxin-like factor (DLF). As this substance crossreacts with digoxin antibodies, it was measured with a radioimmunoassay used to determine exogenous digoxin. Methodological conditions and quality control to determine DLF in plasma and urine have been established. Good correlation coefficients in specificity as well as dilution studies were obtained. Within--and between--assay coefficients of variations indicate good reproducibility. Moreover, changes in plasma DLF levels were detected in patients with cirrhosis or with renal failure, diseases which thrive on alterations in salt and water metabolism. In conclusion, this radioimmunoassay method for measuring DLF may be useful to investigate the role of this factor in several physiological and pathological conditions.
authors.affiliation: Departamento de Bioquímica, Facultad de Medicina, Universidad de Granada, España.
authors.forename: M J
authors.initials: MJ
authors.lastname: Baz
authors.forename: F
authors.initials: F
authors.lastname: Vargas
authors.forename: J M
authors.initials: JM
authors.lastname: Haro
authors.forename: M A
authors.initials: MA
authors.lastname: Castillo
authors.forename: E
authors.initials: E
authors.lastname: Jódar
authors.forename: J L
authors.initials: JL
authors.lastname: Andrade
authors.forename: C
authors.initials: C
authors.lastname: García del Río
mesh_terms: D000293:Adolescent; D000328:Adult; D001798:Blood Proteins / Q000032:analysis* / Q000652:urine; D002298:Cardenolides; D004077:Digoxin; D005260:Female; D006801:Humans; D008297:Male; D011786:Quality Control; D011863:Radioimmunoassay; D012503:Saponins
chemical_list: D001798:Blood Proteins; D002298:Cardenolides; D012503:Saponins; C034504:digoxin-like factors; D004077:Digoxin
vernacular_title: Desarrollo y control de calidad de un radioinmunoensayo para la medida de digoxina endógena.