title: Analysis of dimerization and DNA binding functions in Fos and Jun by domain-swapping: involvement of residues outside the leucine zipper/basic region.
abstract: The products of two cellular proto-oncogenes c-fos and c-jun form a heterodimeric complex that contribute to the DNA-binding activity referred to as AP-1 (activator protein-1). Two domains have been proposed to be required for heterodimer formation and protein-DNA complex formation. The leucine zipper domain mediated the interaction between the two proteins and a highly basic region immediately N-terminal to the leucine zipper forms a DNA binding domain. To assess the role of these two domains in dimerization and DNA binding and to determine what contribution, if any, is made by residues outside of these regions, we carried out an extensive domain swap analysis. Restriction sites created in the fos and jun cDNAs flanking the basic region and leucine zipper allowed these domains to be swapped between the two proteins either singly or in various combinations with adjacent domains. The chimeric proteins were assayed for their ability to dimerize with each other and to interact with the AP-1 consensus sequence. It was found that two Jun leucine zipper regions could mediate protein dimerization, whereas two Fos leucine zipper regions could not. The dimers formed between two Jun leucine repeats were less stable than those formed between a Fos and a Jun leucine zipper. A conserved His residue seven amino acids C-terminal of the last leucine of the zipper region contributed to the stability of protein-protein interactions. The basic region of both Fos and Jun was found to interact with DNA without the presence of the other, i.e. the combination of two Fos- or two Jun-DNA binding domains could bind to the AP-1 site. However, replacement of the Jun N-terminus with that of Fos resulted in a decrease in DNA binding, indicating that residues outside of the Jun basic region contribute to DNA binding. The results also suggest that the dimerization and DNA binding functions of each protein are not completely independent properties, but that each exerts an influence on the other.
authors.affiliation: Department of Molecular Oncology and Virology, Roche Institute of Molecular Biology, Nutley, New Jersey 07110.
authors.forename: D R
authors.initials: DR
authors.lastname: Cohen
authors.forename: T
authors.initials: T
authors.lastname: Curran
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002678:Chimera / Q000235:genetics; D004247:DNA / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D006824:Hybridization, Genetic / Q000235:genetics; D007930:Leucine / Q000032:analysis; D008969:Molecular Sequence Data; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000235:genetics*; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D015183:Restriction Mapping; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D014157:Transcription Factors; D004247:DNA; D007930:Leucine