title: [Deoxyribonuclease from Corynebacterium diphtheriae: dynamics of synthesis and properties].
abstract: Diphtheritic bacteria of PW-8 Massachusetts strain produced into cultural medium only one nucleotidase--endoDNAase. The enzyme was synthesized by the cells during the exponential phase of growth. The DNAase was purified 500-fold and exhibited properties specific to neutral-alkaline DNAases (pH optimum about 7.5, absolute requirements for Me2+, single-step mechanism of substrate hydrolysis). The following properties were typical for the enzyme: absence of distinct specificity to structure of bases surrounding the hydrolyzed bond, formation of 5'-end phosphate groups and slightly higher preference to denatured DNA.
authors.forename: D V
authors.initials: DV
authors.lastname: Iusupova
authors.forename: G P
authors.initials: GP
authors.lastname: Bening
authors.forename: R B
authors.initials: RB
authors.lastname: Sokolova
authors.forename: R V
authors.initials: RV
authors.lastname: Fedorov
mesh_terms: D003353:Corynebacterium diphtheriae / Q000201:enzymology* / Q000254:growth & development; D004269:DNA, Bacterial / Q000378:metabolism; D003851:Deoxyribonucleases / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000378:metabolism; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D014783:Viscosity
chemical_list: D004269:DNA, Bacterial; D003851:Deoxyribonucleases
vernacular_title: Dezoksiribonukleaza difteriĭnykh bakteriĭ: dinamika obrazovaniia i nekotorye svoĭstva.