Expression of heterobispecific antibodies by genes transfected into producer hybridoma cells.
We report the expression of heterobispecific antibodies (Ab) by transferred genes. The kappa and gamma 1 genes of a mouse anti-idiotypic Ab (IgG1) were transfected into a mouse hybridoma cell line secreting Ab (IgG1), directed against an isoenzyme of human creatine kinase. Stable cell lines secreting the parental Ab derived from the introduced genes and a mixture of hybrid Ab were established. The transfected Ab specificity was expressed at similar levels as in a nonproducer background (50 ng-1 microgram/ml), heterobispecific Ab was expressed in microgram quantities (1-4 micrograms/ml) in all cell lines examined. As shown by isoelectric focusing analysis, hybrid Ab (heterobifunctional and other species) are expressed to a similar extent in the transfected cell lines as the Ab in the parental Ab-producing cells.
Lenz
H
H
Biochemical Research Center, Boehringer Mannheim GmbH, Tutzing, F.R.G.
Weidle
U H
UH
D000818:Animals; D000918:Antibody Specificity / Q000235:genetics*; D003001:Cloning, Molecular; D003402:Creatine Kinase / Q000276:immunology; D005803:Genes, Immunoglobulin; D006801:Humans; D006825:Hybridomas; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007130:Immunoglobulin Idiotypes / Q000235:genetics; D007142:Immunoglobulin gamma-Chains / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D007527:Isoenzymes; D051379:Mice; D014162:Transfection
D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes; D007142:Immunoglobulin gamma-Chains; D007145:Immunoglobulin kappa-Chains; D007527:Isoenzymes; D003402:Creatine Kinase