Neural differentiation increases expression of Alzheimer amyloid protein precursor gene in murine embryonal carcinoma cells.
Neural differentiation of the embryonal carcinoma P19 cell line markedly increased the abundance of mRNA encoding Alzheimer amyloid beta/A4-protein precursor (APP). In P19 cells treated with retinoic acid, the abundance of mRNA encoding APP695, which lacks the protease inhibitor domain, reached a maximum on days 2-4 and decreased thereafter, whereas the abundances of mRNAs encoding APP751 and APP770, both possessing the protease inhibitor domain, slowly increased to reach higher levels than APP695 mRNA at later stages of neural differentiation. The induction of APP695 mRNA was consistent with the appearance of neurons in the P19 cultures. A high abundance of APP695 mRNA was also detected in mouse brain at a stage of the period of neuroblast formation. Thus, neural differentiation of P19 cells may present a suitable model for studying the regulation of APP gene expression during early differentiation of brain cells in vivo.
Yoshikawa
K
K
Department of Molecular Biology, Psychiatric Research Institute of Tokyo, Japan.
Aizawa
T
T
Maruyama
K
K
D000199:Actins / Q000235:genetics; D000544:Alzheimer Disease / Q000378:metabolism*; D000682:Amyloid / Q000235:genetics* / Q000378:metabolism; D016229:Amyloid beta-Peptides; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000196:embryology*; D002454:Cell Differentiation / Q000187:drug effects; D005786:Gene Expression Regulation; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics; D007381:Intermediate Filament Proteins / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016900:Neurofilament Proteins; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000235:genetics; D013724:Teratoma; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000199:Actins; D000682:Amyloid; D016229:Amyloid beta-Peptides; D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D014212:Tretinoin