The effects of the preincubation with vitamin B2-acid on ferredoxin-NADP+ reductase.
The effects of vitamin B2-acid on both cytochrome c reductase and diaphorase activities of ferredoxin-NADP+ reductase [EC 1.18.1.2] were investigated with enzyme kinetics. Vitamin B2-acid was shown to serve as an electron carrier for the two activities, as well as riboflavin or flavin mononucleotide (FMN). The two activities, however, were irreversibly affected by the preincubation of the enzyme with vitamin B2-acid under certain conditions, while riboflavin or FMN did not show such effects.
Kato
M
M
Department of Chemistry, Faculty of Science and Engineering, Ritsumeikan University, Kyoto, Japan.
Tachibana
S
S
D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism; D005287:Ferredoxin-NADP Reductase / Q000378:metabolism*; D007700:Kinetics; D009245:NADH Dehydrogenase / Q000378:metabolism; D012256:Riboflavin / Q000031:analogs & derivatives* / Q000378:metabolism
C023848:vitamin B2 acid; D005287:Ferredoxin-NADP Reductase; D009245:NADH Dehydrogenase; D008058:Dihydrolipoamide Dehydrogenase; D012256:Riboflavin