A role for cytokines in antigen presentation: IL-1 and IL-4 induce accessory functions of antigen-presenting cells.
Fixation of APC with 1-ethyl-3-(3-dimethylamino-propyl)carbodiimide (ECDI) eliminates their ability to stimulate proliferation of alloreactive T cells or the D10 T cell clone, although a partial response, IL-4 production, was measured. However, if APC were activated before fixation, they could be ECDI-fixed and retain the ability to induce T cell proliferation. IL-1, IL-4 or LPS were capable of activating APC in this way, whereas IFN-gamma was not. This activation step occurred in 6 h, required protein synthesis, and was distinct from increases in Ia or IL-1. This suggests resting APC lack structures that are essential for inducing T cell proliferation.
Aiello
F B
FB
Biological Carcinogenesis and Development Program, NCI-Frederick Cancer Research Facility, MD 21701-1013.
Longo
D L
DL
Overton
R
R
Takacs
L
L
Durum
S K
SK
D000818:Animals; D000938:Antigen-Presenting Cells / Q000502:physiology*; D001685:Biological Factors / Q000502:physiology; D003513:Cycloheximide / Q000494:pharmacology; D016207:Cytokines; D005404:Fixatives; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007108:Immune Tolerance; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D051379:Mice; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology*
D001685:Biological Factors; D016207:Cytokines; D005404:Fixatives; D000949:Histocompatibility Antigens Class II; D007375:Interleukin-1; D008070:Lipopolysaccharides; D015847:Interleukin-4; D007371:Interferon-gamma; D003513:Cycloheximide