High molecular weight aggregate content of heated and unheated factor VIII products determined by fast-protein liquid chromatography.
The molecular-weight distribution of proteins in factor VIII concentrates has been analysed by fast-protein liquid chromatography. The proportion of high-molecular-weight (HMW) aggregates in one product increased on freeze-drying and heating, with fibrinogen and fibronectin being the main protein components of the HMW peak. In all other concentrates, the HMW peak was less than or equal to 5% of the total protein content and there were no differences in HMW content according to purity or method of viral inactivation.
Dawes
J
J
Heart Research Institute, Sydney, Australia.
Freeman
L
L
Dawson
N J
NJ
Pepper
D S
DS
Barrowcliffe
T W
TW
D002853:Chromatography, Liquid / Q000379:methods; D005169:Factor VIII / Q000032:analysis*; D006358:Hot Temperature; D006801:Humans; D008970:Molecular Weight
D005169:Factor VIII