Use of Saccharomyces cerevisiae expressing beta-galactosidase to screen for antimycotic agents directed against yeast cell wall biosynthesis and possible application to pathogenic fungi.
Saccharomyces cerevisiae expressing beta-galactosidase was used to develop a screen for compounds active against formation of the yeast cell wall. The screen detailed here is based on the release of beta-galactosidase from cells which had received an osmotic shock.
Zaworski
P G
PG
Molecular Biology Research, Upjohn Company, Kalamazoo, Michigan 49007.
Gill
G S
GS
D000935:Antifungal Agents / Q000494:pharmacology*; D002473:Cell Wall / Q000187:drug effects; D005696:Galactosidases / Q000378:metabolism*; D009997:Osmotic Pressure; D010456:Peptides, Cyclic; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000378:metabolism; D013012:Sorbitol / Q000494:pharmacology*; D001616:beta-Galactosidase / Q000378:metabolism*
D000935:Antifungal Agents; D010456:Peptides, Cyclic; D013012:Sorbitol; C011634:aculeacin A; D005696:Galactosidases; D001616:beta-Galactosidase