title: Activation of promutagenic chemicals by Streptomyces griseus containing cytochrome P-450soy.
abstract: Streptomyces griseus cells containing cytochrome P-450soy oxidize a diverse array of xenobiotic compounds. This metabolic capability was exploited for activation of promutagenic chemicals such as polycyclic aromatic hydrocarbons, aromatic amines and small aliphatics in a modified Salmonella/Ames plate incorporation assay using tester strains TA98 and TA1538. In this assay promutagens such as 3,3'-dimethylbenzidine, 3,3'-dimethoxybenzidine, benzidine, 2-acetylaminofluorene, 2-aminoanthracene, 2,4-diaminotoluene, 4-aminobiphenyl, benzo(a)pyrene, chloropicrin and N-nitrosodimethylamine were oxidized to mutagenic metabolites by S. griseus intact cells which mutated Salmonella tester strains (TA98 and TA1538). S. griseus failed to activate 7,12-dimethylbenzanthracene and 4-chloro-2-nitroaniline. In parallel tests performed with rat liver homogenate (S9), N-nitrosodimethylamine was not activated.
authors.affiliation: Central Research & Development Dept., E.I. duPont de Nemours & Co., Wilmington, Delaware 19880-0228.
authors.forename: F S
authors.initials: FS
authors.lastname: Sariaslani
authors.forename: R G
authors.initials: RG
authors.lastname: Stahl
mesh_terms: D001711:Biotransformation; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004789:Enzyme Activation; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000378:metabolism*; D013025:Glycine max; D013305:Streptomyces griseus / Q000378:metabolism*
chemical_list: D009153:Mutagens; D003577:Cytochrome P-450 Enzyme System