title: Short-term stimulatory effect of Sertoli cell conditioned medium on Leydig cell steroidogenesis is not mediated by inhibin.
abstract: Addition of concentrated rat Sertoli cell conditioned medium (rSCCM) to isolated Leydig cells from immature rats stimulated steroid production more than 13-fold within 4 h. LH-stimulated steroidogenesis was not enhanced by addition of rSCCM. The biological activity of the concentrated rSCCM was higher after incubation of Sertoli cells with FSH, whereas FSH alone did not stimulate steroid production. This effect of rSCCM was not due to inhibin, since highly purified 32 kDa rat inhibin, in doses equivalent to those present in rSCCM, had no effect on steroidogenesis during the 4 h incubation period. Furthermore, inhibin could be separated from the Leydig cell stimulating factor by anion-exchange chromatography. These results indicate a short-term paracrine control of Leydig cell steroidogenesis by Sertoli cell derived factors, which differ from inhibin.
authors.affiliation: Department of Biochemistry, Erasmus University Rotterdam, The Netherlands.
authors.forename: A J
authors.initials: AJ
authors.lastname: Grootenhuis
authors.forename: R
authors.initials: R
authors.lastname: Melsert
authors.forename: M A
authors.initials: MA
authors.lastname: Timmerman
authors.forename: J W
authors.initials: JW
authors.lastname: Hoogerbrugge
authors.forename: F F
authors.initials: FF
authors.lastname: Rommerts
authors.forename: F H
authors.initials: FH
authors.lastname: de Jong
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D007265:Inhibins / Q000302:isolation & purification / Q000494:pharmacology / Q000502:physiology*; D007700:Kinetics; D007985:Leydig Cells / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011284:Pregnenolone / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D012708:Sertoli Cells / Q000502:physiology*; D013256:Steroids / Q000096:biosynthesis
chemical_list: D003470:Culture Media; D013256:Steroids; D007265:Inhibins; D011284:Pregnenolone; D005640:Follicle Stimulating Hormone