Continuous in-vitro cultivation of Plasmodium falciparum in Ibadan: solutions to scientific and logistical problems.
The technique of continuous in-vitro cultivation of Plasmodium falciparum has not been widely applied in malaria-endemic areas, due to scientific as well as purely logistical problems. Methods for solving or coping with these problems are described. They have already proved effective for over 4 years. The parasites harvested have been satisfactory, as judged by reproducible logarithmic growth curves and normal morphology in Leishman-stained smears as well as phase-contrast microscopy of wet-preparations. They have also been successfully used as antigen sources in the malarial fluorescent-antibody test and to investigate enhanced platelet aggregation in experimental malaria.
Sodeinde
O
O
Department of Paediatrics, College of Medicine, University of Ibadan, Nigeria.
Williams
C K
CK
D000818:Animals; D003470:Culture Media; D005455:Fluorescent Antibody Technique; D066298:In Vitro Techniques; D008288:Malaria / Q000175:diagnosis; D009549:Nigeria; D010274:Parasitology / Q000379:methods; D010963:Plasmodium falciparum / Q000032:analysis / Q000254:growth & development*; D010974:Platelet Aggregation; D012106:Research
D003470:Culture Media