[An immunochemical analysis of the induction of cytochrome P-450 isoforms in the liver of fresh-water fishes by 3-methylcholanthrene, beta-naphthoflavone and aroclor 1254].
The cytochrome P-450 isoforms have been studied in liver microsomes of some fish species from Lake Baikal. Using the inhibitory analysis of microsomal monooxygenase activities carried out by the specific polyclonal antibodies it has been shown that 3-methylcholanthrene, beta-naphthoflavone and arochlor 1254 induce isoforms immunologically related to cytochrome P-488c but not to the rat cytochrome P-450b in fish liver microsomes. The immunologic identity in isoforms of fish and rat cytochromes induced by methylcholanthrene has not been revealed. A possibility to use the method of the inhibitory analysis of fish microsomal activities by specific antibodies to the rat cytochrome P-450 isoforms for biomonitoring and biotesting of polycyclic hydrocarbons and polychlorinated biphenyls in aquatic systems is discussed.
Kotelevtsev
S V
SV
Ponomarëva
L V
LV
Novikov
K N
KN
Viner
R I
RI
Kozlov
Iu P
IuP
Khatsenko
O G
OG
Mitrofanov
D V
DV
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000918:Antibody Specificity / Q000276:immunology; D001140:Aroclors / Q000494:pharmacology*; D001571:Benzoflavones / Q000494:pharmacology*; D020111:Chlorodiphenyl (54% Chlorine); D003577:Cytochrome P-450 Enzyme System / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology; D004790:Enzyme Induction / Q000187:drug effects; D005399:Fishes / Q000378:metabolism*; D005419:Flavonoids / Q000494:pharmacology*; D005618:Fresh Water; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D008748:Methylcholanthrene / Q000494:pharmacology*; D008862:Microsomes, Liver / Q000032:analysis / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology; D011078:Polychlorinated Biphenyls / Q000494:pharmacology*; D019324:beta-Naphthoflavone
D000906:Antibodies; D001140:Aroclors; D001571:Benzoflavones; D005419:Flavonoids; D007527:Isoenzymes; D020111:Chlorodiphenyl (54% Chlorine); D008748:Methylcholanthrene; D019324:beta-Naphthoflavone; D003577:Cytochrome P-450 Enzyme System; D011078:Polychlorinated Biphenyls
Immunokhimicheskiĭ analiz induktsii izoform tsitokhrom P-450 v pecheni presnovodnykh ryb 3-metilkholantrenom, beta-naftoflavonom i arokhlorom 1254.