title: Isolation of Chinese hamster ovary cells with reduced poly(ADP-ribose) polymerase activity.
abstract: The biological function of poly(ADP-ribose) polymerase in DNA repair, cell-cycle regulation and cellular differentiation has yet to be defined. Isolation of cells which are deficient in poly(ADP-ribose) synthesis would greatly facilitate the determination of the biological role of this enzyme. A method is described for isolating Chinese hamster ovary (CHO) cells deficient in the poly(ADP-ribose) polymerase activity by direct screening of colonies for enzyme activity. Colonies with decreased production of poly(ADP-ribose) are recovered from nylon replicas for further analysis. Using this method we have isolated a series of CHO cells which have 50% or less poly(ADP-ribose) polymerase activity. These mutants have normal generation times and are 20% more sensitive to the effects of DNA (m)ethylating agents than the parental cell. However, these mutants display normal sensitivity to gamma-rays.
authors.affiliation: Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
authors.forename: R A
authors.initials: RA
authors.lastname: MacLaren
authors.forename: M V
authors.initials: MV
authors.lastname: Witmer
authors.forename: E
authors.initials: E
authors.lastname: Richardson
authors.forename: T D
authors.initials: TD
authors.lastname: Stamato
mesh_terms: D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D006224:Cricetinae; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D007700:Kinetics; D009154:Mutation; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism*
chemical_list: D005020:Ethyl Methanesulfonate; D011065:Poly(ADP-ribose) Polymerases