Dose-effect relationship for X-ray-induced reciprocal translocations in mouse spermatogonia following pretreatment with 3-aminobenzamide.
The influence of 3-aminobenzamide (3-AB) pretreatment on the dose-response relationship for radiation-induced reciprocal translocations in mouse spermatogonial stem cells was studied. The results show that at doses of 3-10 Gy of X-rays the frequencies of translocations were higher in 3-AB-pretreated animals as compared to animals that received X-rays only. The 3-AB pretreatment was not effective at dose levels of 1 and 2 Gy. The shape of the dose-effect curve was similar to that obtained without 3-AB pretreatment, i.e., a humped curve, but the initial slope was clearly steeper and the position of the peak was shifted from 7 to 9 Gy. The effects observed can be explained by a 3-AB-mediated sensitization of normally radioresistant stem cells that are at the stage of stimulation to enter the mitotic cycle, thus increasing the population of radiosensitive spermatogonial stem cells.
van Buul
P P
PP
Department of Radiation Genetics and Chemical Mutagenesis, Sylvius Laboratory, Leiden, The Netherlands.
Léonard
A
A
Goudzwaard
J H
JH
D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D004307:Dose-Response Relationship, Radiation; D008297:Male; D051379:Mice; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D013093:Spermatogonia / Q000187:drug effects / Q000528:radiation effects*; D014178:Translocation, Genetic / Q000187:drug effects / Q000528:radiation effects*; D014965:X-Rays
D001549:Benzamides; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; C025160:3-aminobenzamide