Interleukin-1 and tumor necrosis factor synthesis by mouse peritoneal macrophages is enhanced by dietary n-3 polyunsaturated fatty acids.
The peritoneal macrophages from mice maintained for 16 days on a diet containing (10%) menhaden oil contained less arachidonic acid and more n-3 polyunsaturated fatty acids (n-3 PUFA) than those maintained on diets containing an equivalent amount of corn oil. Following stimulation with lipopolysaccharide, the production of PGE2, interleukin-1 (IL-1) and tumor necrosis factor (TNF) was 2.1 vs. 5.3 ng PGE2/micrograms DNA; 685 vs. 30 units IL-1/micrograms DNA and 14 vs. less than 4 units TNF by macrophages from mice consuming menhaden and corn oil, respectively. Macrophages from animals on diets containing olive oil generated intermediate amounts of PGE2 and equivalent amounts of IL-1 and TNF to those on corn oil. The data indicate that dietary n-3 PUFA at specific intake levels relative to n-6 PUFA may enhance cytokine generation by reducing PGE2 synthesis.
Lokesh
B R
BR
Lipid Research Laboratory, Cornell University, Ithaca, NY 14853.
Sayers
T J
TJ
Kinsella
J E
JE
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000032:analysis; D004041:Dietary Fats / Q000494:pharmacology*; D015232:Dinoprostone / Q000096:biosynthesis; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D005395:Fish Oils / Q000494:pharmacology*; D007375:Interleukin-1 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D010938:Plant Oils / Q000494:pharmacology; D013268:Stimulation, Chemical; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D001095:Arachidonic Acids; D004041:Dietary Fats; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D007375:Interleukin-1; D008070:Lipopolysaccharides; D010938:Plant Oils; D014409:Tumor Necrosis Factor-alpha; C033266:Menhaden oil; D016718:Arachidonic Acid; D015232:Dinoprostone