Immunohistochemical studies of the carbonic anhydrase isozymes in the bovine placenta.
Placentae from 11 cows, ranging from about 80 to 270 days gestation, were studied for immunohistochemical localization of carbonic anhydrase isozymes. CA isozymes were localized using the avidin-biotin-peroxidase complex (ABC) method in the bovine placenta. CA-II was found in the fetal trophoblastic cells with single nuclei as well as in erythrocytes of maternal and fetal blood. The percentage of positive cells for anti-CA-II in the trophoblastic cells did not change during development of gestation in the fetal bovine. CA-I and CA-III were not detected in the bovine placenta.
Nishita
T
T
Department of Physiology I, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
Kinoshita
C
C
Maegaki
M
M
Asari
M
M
D000818:Animals; D002256:Carbonic Anhydrases / Q000096:biosynthesis*; D002417:Cattle; D005260:Female; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000096:biosynthesis*; D010920:Placenta / Q000166:cytology / Q000201:enzymology*; D011247:Pregnancy; D014327:Trophoblasts / Q000201:enzymology
D007527:Isoenzymes; D002256:Carbonic Anhydrases