title: T cells and neutrophils exhibit differential adhesion to cytokine-stimulated endothelial cells.
abstract: In vitro adhesion assays were used to directly compare the adhesion of polymorphonuclear leucocytes (PMN) and T cells to endothelial cells (EC). PMN exhibited lower binding to unstimulated EC than T cells. When EC were stimulated with interleukin-1 (IL-1), tumour necrosis factor (TNF) or bacterial lipopolysaccharide (LPS) there was a large and rapid increase in adhesiveness for PMN which peaked at 4 hr. This had fallen significantly by 24 hr and by 72 hr was not significantly elevated above unstimulated adhesion. The increase in adhesiveness of cytokine-stimulated EC for T cells was smaller and more gradual than for PMN, with adhesion peaking around 8 hr and remaining significantly elevated at 72 hr. In contrast, interferon-gamma (IFN-gamma) enhanced EC adhesiveness for T cells but not for PMN, with maximal T cell EC adhesiveness occurring 24 hr after stimulation. As leucocyte adhesion to vascular endothelium is the first step in diapedesis, differences in PMN and T-cell adhesion to EC may be important in determining the timing and composition of inflammatory infiltrates.
authors.affiliation: Rheumatology Unit, Guy's Hospital, London, U.K.
authors.forename: M H
authors.initials: MH
authors.lastname: Thornhill
authors.forename: U
authors.initials: U
authors.lastname: Kyan-Aung
authors.forename: T H
authors.initials: TH
authors.lastname: Lee
authors.forename: D O
authors.initials: DO
authors.lastname: Haskard
mesh_terms: D001685:Biological Factors; D002448:Cell Adhesion; D002478:Cells, Cultured; D016207:Cytokines; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D007371:Interferon-gamma; D007375:Interleukin-1; D008070:Lipopolysaccharides; D009504:Neutrophils / Q000502:physiology*; D011994:Recombinant Proteins; D013601:T-Lymphocytes / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha
chemical_list: D001685:Biological Factors; D016207:Cytokines; D007375:Interleukin-1; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma