Cloning and expression in Escherichia coli of tryptophan genes from Streptomyces griseus IMRU 3570.
Two Sau3A fragments of Streptomyces grisues IMRU 3570 were cloned in pBR322 as a vector. One of these clones contained the genetic information needed to complement trpA and trpB mutations in Escherichia coli. The other complements trpA, trpB and trpC mutations in E. coli. Both fragments originated in the same region of the chromosome but the latter is 1 kilobase (kb) longer in the region nearest the tetracycline promoter.
Rivero-Lezcano
O
O
Departamento de Patología Animal (Sanidad Animal), Unidad de Microbiología, Facultad de Veterinaria, Universidad de León, Spain.
Anguita-Castillo
J
J
López-Nieto
J
J
Naharro-Carrasco
G
G
D015139:Blotting, Southern; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D015183:Restriction Mapping; D013305:Streptomyces griseus / Q000235:genetics*; D014364:Tryptophan / Q000096:biosynthesis / Q000235:genetics*
D004269:DNA, Bacterial; D014364:Tryptophan