Radioimmunoassay of 3 alpha-hydroxy-5 alpha-pregnan-20-one in rat and human plasma.
A radioimmunoassay for measuring 3 alpha-hydroxy-5 alpha-pregnan-20-one in plasma has been developed. Polyclonal antibodies were raised in rabbits against 3 alpha-hydroxy-20-oxo-5 alpha-pregnan-11 alpha-yl carboxymethyl ether coupled to bovine serum albumin. 3 alpha-Hydroxy-5 alpha-pregnan-20-one was purified from either extracts of plasma by high-performance liquid chromatography. These antibodies were then used for the radioimmunoassay of this centrally active progesterone metabolite in rat and human plasma. 3 alpha-Hydroxy-5 alpha-pregnan-20-one was detected in plasma from female rats on the day of estrus (2.0 to 9.3 ng/ml) and in the plasma of women during the luteal phase of the menstrual cycle at levels ranging from 0.25 to 2.5 ng/ml. The latter was highly correlated with plasma progesterone levels.
Purdy
R H
RH
Department of Organic Chemistry, Southwest Foundation for Biomedical Research, San Antonio, Texas 78284.
Moore
P H
PH
Rao
P N
PN
Hagino
N
N
Yamaguchi
T
T
Schmidt
P
P
Rubinow
D R
DR
Morrow
A L
AL
Paul
S M
SM
D000818:Animals; D000906:Antibodies; D002851:Chromatography, High Pressure Liquid; D004971:Estrus; D005260:Female; D006801:Humans; D008183:Luteal Phase; D011280:Pregnanolone / Q000097:blood*; D011374:Progesterone / Q000097:blood; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D000906:Antibodies; D011374:Progesterone; D011280:Pregnanolone