The use of diphenylene iodonium, an inhibitor of NADPH oxidase, to investigate the antimicrobial action of human monocyte derived macrophages.
Diphenylene iodonium is an inhibitor of the respiratory burst-generating NADPH oxidase of phagocytes. The effect of this compound on human monocyte-derived macrophages and its usefulness in exploring the antimicrobial mechanisms of phagocytes was examined. 1 microM diphenylene iodonium inhibited hydrogen peroxide production by human macrophages and the activity of these cells against Toxoplasma gondii. At this concentration macrophage degranulation was unaffected.
Robertson
A K
AK
Department of Microbiology, University of Leicester, U.K.
Cross
A R
AR
Jones
O T
OT
Andrew
P W
PW
D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D015550:Cell Degranulation; D002469:Cell Separation; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007371:Interferon-gamma / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism / Q000382:microbiology; D008297:Male; D051379:Mice; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors*; D019255:NADPH Oxidases; D009861:Onium Compounds / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism; D010586:Phagocytes / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D011994:Recombinant Proteins; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014122:Toxoplasma / Q000502:physiology*
D009861:Onium Compounds; D011994:Recombinant Proteins; C007517:diphenyleneiodonium; D007371:Interferon-gamma; D006861:Hydrogen Peroxide; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases; D000118:Acetylglucosaminidase; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen