Chemical and physical properties of the major serum albumin adduct of aflatoxin B1 and their implications for the quantification in biological samples.
Aflatoxin B1 (AFB1) is a potent carcinogen. It reacts with liver DNA and serum albumin in a dose-dependent manner. Serum albumin adducts of aflatoxins have been used for exposure assessment. The immunological methods used so far do not differentiate between the different adducts of AFB1 and of AFG1. In order to establish an analytical method to measure one specific AFB1 adduct, we investigated the structure and the chemistry of the major serum albumin adduct of aflatoxin B1 (lysine-AFB1). 13C-NMR, 1H-NMR, UV, IR, fluorescence and MS spectra of lysine-AFB1 (8-[N-(2-amino-hexanoyl-6-yl)-5-oxo-3-pyrrolin-3-yl]-7-hydroxy-5- methoxycyclopentenone[2.3-c]coumarin) were recorded and discussed. The quantification of lysine-AFB1 was demonstrated in biological samples. Serum albumin was digested with pronase and analysed by HPLC with a fluorimeter as detector. The detection limit found for lysine-AFB1 was 20 fmol.
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Institute of Pharmacology and Toxicology, University of Würzburg, F.R.G.
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000378:metabolism* / Q000633:toxicity; D000818:Animals; D002273:Carcinogens / Q000378:metabolism* / Q000633:toxicity; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D004784:Environmental Monitoring; D006801:Humans; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000187:drug effects / Q000378:metabolism*
D000348:Aflatoxins; D002273:Carcinogens; D012709:Serum Albumin; D016604:Aflatoxin B1