title: Influence of whole body irradiation and local shielding on matrix-induced endochondral bone differentiation.
abstract: Subcutaneous implantation of demineralized bone matrix into allogeneic rats induces endochondral bone formation. We have investigated the effects of irradiation on the sequelae of the interaction of collagenous matrix and mesenchymal cells and on cartilage and bone differentiation. Rats were irradiated in a vertical direction with a midline dose of 850 rad. Radiation entered the rats ventrally while a small area of the upper thorax was locally shielded. After irradiation, bone matrix was implanted in shielded and nonshielded sites, and the implants were studied at various stages. On day 3, [3H]thymidine incorporation, an index of cell proliferation, was inhibited by 70% in the nonshielded sites compared to nonirradiated control rats. The degree of inhibition (35%) was less pronounced in shielded sites. Furthermore, there was recovery of cell proliferation in the shielded sites as opposed to the nonshielded contralateral site. A similar pattern was observed on day 7 as assessed by 35SO4 incorporation into proteoglycans during chondrogenesis. Bone formation and mineralization were quantified on day 11 by alkaline phosphatase activity and 45Ca incorporation. In nonshielded sites, there was a 73% inhibition of alkaline phosphatase activity. In conclusion, radiation impaired progenitor cell proliferation which resulted in decreased cartilage and bone differentiation. These findings imply that local mesenchymal cells proliferate and differentiate into bone in response to implanted collagenous matrix.
authors.affiliation: Bone Cell Biology Section, National Institute of Dental Research, Bethesda, MD 20892.
authors.forename: S
authors.initials: S
authors.lastname: Wientroub
authors.forename: J F
authors.initials: JF
authors.lastname: Weiss
authors.forename: G N
authors.initials: GN
authors.lastname: Catravas
authors.forename: A H
authors.initials: AH
authors.lastname: Reddi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001846:Bone Development / Q000528:radiation effects*; D001857:Bone Matrix / Q000378:metabolism; D002113:Calcification, Physiologic / Q000528:radiation effects; D002118:Calcium / Q000032:analysis / Q000378:metabolism; D008297:Male; D019736:Prostheses and Implants; D011835:Radiation Protection; D051381:Rats; D013997:Time Factors; D014916:Whole-Body Irradiation
chemical_list: D001672:Biocompatible Materials; D002118:Calcium