title: Characterization of tau protein present in microtubules and paired helical filaments of Alzheimer's disease patient's brain.
abstract: Two proteins immunologically related to porcine tau protein are found in the brain of Alzheimer's disease patients. One is bound to microtubules and, after isolation by co-polymerization with tubulin, shows a size and tryptic peptide map, similar to the microtubule-associated tau protein, present in the brain of non-demented patients. The other tau-related protein is present as the major protein of a purified fraction of paired helical filaments. The paired helical filament-associated protein shows smaller molecular weight (33,000) than microtubule-associated tau; however, this 33,000 mol. wt protein reacts with a monospecific anti-tau antibody and with an antibody to a 19-amino acid peptide corresponding to amino acids 228-246 of human tau. Furthermore, the 33,000 mol. wt protein and the tau protein have similar tryptic peptide maps. These results suggest that the paired helical filament protein is a modified form of the microtubule-associated tau protein.
authors.affiliation: Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Nieto
authors.forename: E
authors.initials: E
authors.lastname: Montejo de Garcini
authors.forename: I
authors.initials: I
authors.lastname: Correas
authors.forename: J
authors.initials: J
authors.lastname: Avila
mesh_terms: D000368:Aged; D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000473:pathology; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D008869:Microtubule-Associated Proteins / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D008870:Microtubules / Q000737:chemistry* / Q000378:metabolism; D008875:Middle Aged; D008969:Molecular Sequence Data; D008970:Molecular Weight; D013552:Swine; D016875:tau Proteins
chemical_list: D008869:Microtubule-Associated Proteins; D016875:tau Proteins