title: Interaction between two distinct plaque-cloned human immunodeficiency viruses (HIVs): the possible existence of heterozygous virus.
abstract: To know the biological significance of the HIV variation, we investigated the interaction between two different HIV clones. Two distinct clones were mixed and propagated by infecting MT-4 cells. After passaging the mixture viruses 8 times, and cloning by the plaque method, we obtained not only viruses of homogeneous type like each parental clone but also heterogeneous-type viruses which showed a mixture of two parental viruses with regard to phenotype and genotype. To further segregate a single virus from the mixture, we recloned the heterogeneous viruses using the plaque method. We found that about 40% of recloned viruses from heterogeneous-type viruses were still heterogeneous.
authors.affiliation: Department of Biodefence and Medical Virology, Kumamoto University Medical School.
authors.forename: T
authors.initials: T
authors.lastname: Masuda
authors.forename: S
authors.initials: S
authors.lastname: Harada
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis; D014644:Genetic Variation; D005838:Genotype; D015497:HIV-1 / Q000235:genetics* / Q000254:growth & development; D010641:Phenotype; D011533:Proviruses / Q000235:genetics / Q000254:growth & development; D015183:Restriction Mapping; D010948:Viral Plaque Assay
chemical_list: D004279:DNA, Viral