Drosophila cell extracts contain a TGF-beta-like activity.
Extracts of the Drosophila cell line KCo contain a TGF-beta-like activity. This bioactivity, like mammalian TGF-beta, induces the anchorage-independent growth of rat NRK-49F cells in the presence of EGF, inhibits 3H-thymidine incorporation in the mink epithelial cell line CCL-64 and is acid and heat stable but destroyed by dithiothreitol. On a reverse phase column a single bioactive peak eluted at 37-40% acetonitrile. This TGF-beta-like activity in KCo cells inhibits the growth of the homotypic Drosophila cell line 14-11-23, whereas porcine TGF-beta 1 had no effect.
Benzakour
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O
Institut Curie, UA 532 du CNRS, Centre Universitaire, Orsay, France.
Echalier
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G
Lawrence
D A
DA
D000818:Animals; D001669:Biochemical Phenomena; D001671:Biochemistry; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000096:biosynthesis; D004331:Drosophila melanogaster / Q000032:analysis*; D066298:In Vitro Techniques; D051381:Rats; D015291:Transforming Growth Factors / Q000032:analysis*
D015291:Transforming Growth Factors; D004247:DNA