Immunomodulatory characteristics of a novel antiproliferative protein, suppressin.
We investigated the immunoregulatory properties of a recently described inhibitor of lymphocyte proliferation, suppressin (SPN). It was determined that preincubation of murine leukocytes with SPN enhances natural killer cell (NK) activity. In addition, SPN potentiates interferon-gamma (IFN-gamma) augmentation of NK activity. Furthermore, preincubation of murine leukocytes with SPN induces the production of IFN-alpha/beta. The IFN-alpha/beta produced is active in NK assays as well as vesicular stomatitis virus neutralization assays. In vivo, SPN increases the time of survival of C57BL/6 mice injected with EL-4 lymphoma cells. Interestingly, SPN inhibits immunoglobulin (IgA, IgG, and IgM) production in response to the mitogen, concanavalin A in a dose-dependent manner. Collectively, the above data indicate SPN may have numerous applications in clinical science including tumor surveillance and autoimmune diseases such as arthritis.
Carr
D J
DJ
Department of Physiology and Biophysics, University of Alabama, Birmingham 35294.
Blalock
J E
JE
Green
M M
MM
LeBoeuf
R D
RD
D000818:Animals; D005260:Female; D007166:Immunosuppressive Agents / Q000276:immunology*; D007371:Interferon-gamma / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype; D013951:Thymus Hormones / Q000276:immunology*
D007166:Immunosuppressive Agents; D013951:Thymus Hormones; C053750:suppressin; D007371:Interferon-gamma