Inhibition of BFU-E in vitro growth and of burst-promoting activity of T lymphocytes by serum of chronic uremic patients receiving hemodialysis.
The effects of uremic serum on the in vitro growth of normal BFU-E and on the burst-promoting activity by normal T-lymphocytes were evaluated separately. The effect of hemodialysis on the removal of possible serum inhibitor(s) was also tested. Sera of 12 uremic patients were shown to provoke a 60% inhibition of the in vitro growth of normal BFU-E and almost complete abolition of burst-promoting activity by T lymphocytes. While hemodialysis significantly removed the inhibition of uremic sera on BFU-E growth, it was rather ineffective in removing the inhibitor(s) of T lymphocytes. The different effects of uremic serum on BFU-E and on T lymphocytes are discussed.
Morra
L
L
Istituto Scientifico di Medicina Interna, Università di Genova, Italia.
Ponassi
A
A
Moccia
F
F
Gurreri
G
G
Bessone
G
G
Mela
G S
GS
Sacchetti
C
C
D000328:Adult; D000368:Aged; D002455:Cell Division; D002469:Cell Separation / Q000379:methods; D002908:Chronic Disease; D003114:Colony-Forming Units Assay / Q000379:methods; D015672:Erythroid Precursor Cells / Q000166:cytology*; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis; D013601:T-Lymphocytes / Q000502:physiology*; D014511:Uremia / Q000097:blood* / Q000628:therapy