Modulation of pteroylpolyglutamate concentration and length in response to altered folate nutrition in a comprehensive range of rat tissues.
For a range of rat tissue extracts, the concentrations of total folates and of short-chain pteroylpolyglutamates were assayed by Lactobacillus casei with and without conjugase treatment, respectively, and the concentration and chain length of H4PteGlnn and 5,10-CH2-H4PteGlnn together were assayed after binding to thymidylate synthase and tritiated fluorodeoxyuridylate. For rats fed a nonpurified diet and consuming 26 micrograms of folic acid daily, the respective concentrations of these total folates, short-chain folates and thymidylate synthase bindable folates were, in nmol/g, 10.2, 2.5 and 3.5 in liver, 3.9, 1.8 and 2.0 in kidney, 4.2, 1.2 and 1.0 in bone marrow, 2.3, 0.6 and 0.2 in adrenal, 2.1, 0.3 and 0.5 in spleen, 2.1, 0.9 and 0.8 in jejunal smooth muscle, 1.2, 0.9 and 0.2 in jejunal mucosa, 1.0, 0.3 and 0.6 in testis, 0.7, 0.1 and 0.2 in heart, 0.3, 0.1 and 0.1 in skeletal muscle, 0.5, 0.1 and 0.3 in brain and 0.7, 0.002 and 0 in erythrocytes. The predominant pteroylpolyglutamate chain length was 6 residues in all tissues except kidney, jejunal mucosa, skeletal muscle and brain, in which the value was 5 residues. A folate-deficient diet (30 ng/d) fed for 3 wk resulted in a depression in the total folate concentration of all tissues (except brain); the depression was generally greater for short-chain than for long-chain folates and was accompanied by a lengthening of the pteroylpolyglutamate chain. Opposite results followed folate excess of 4 to 5.4 mg/d. The fractional change in the folate concentration of the individual tissues, following perturbation of dietary folate, did not vary greatly among tissues.(ABSTRACT TRUNCATED AT 250 WORDS)
Ward
G J
GJ
Department of Biochemistry, University of Queensland, St. Lucia, Australia.
Nixon
P F
PF
D000818:Animals; D004032:Diet; D004591:Electrophoresis, Polyacrylamide Gel; D005492:Folic Acid / Q000008:administration & dosage* / Q000031:analogs & derivatives* / Q000032:analysis / Q000493:pharmacokinetics; D005494:Folic Acid Deficiency / Q000378:metabolism*; D007780:Lacticaseibacillus casei / Q000378:metabolism; D008297:Male; D008970:Molecular Weight; D009752:Nutritional Status; D011624:Pteroylpolyglutamic Acids / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution; D011623:gamma-Glutamyl Hydrolase
D011624:Pteroylpolyglutamic Acids; D005492:Folic Acid; D011623:gamma-Glutamyl Hydrolase