title: Determination of albuterol concentrations in human plasma using solid-phase extraction and high-performance liquid chromatography with fluorescence detection.
abstract: A fast, simple, and accurate method for determining albuterol concentrations in human plasma has been developed and validated for use in routine clinical analyses. This method involves a solid-phase extraction procedure using silica cartridges and normal-phase high-performance liquid chromatography with fluorescence detection. Regression analysis showed the method was linear over the standard curve range 1-16 ng ml-1. The percent recovery for albuterol and the internal standard, bamethane, at 5 ng ml-1 was found to be greater than 90%. The newly developed method has been applied in the analysis of plasma samples from patients and healthy volunteers.
authors.affiliation: Department of Biochemical Pharmacology, Glaxo Group Research Ltd, Ware, Herts, UK.
authors.forename: R E
authors.initials: RE
authors.lastname: Bland
authors.forename: R J
authors.initials: RJ
authors.lastname: Tanner
authors.forename: W H
authors.initials: WH
authors.lastname: Chern
authors.forename: J R
authors.initials: JR
authors.lastname: Lang
authors.forename: J R
authors.initials: JR
authors.lastname: Powell
mesh_terms: D000420:Albuterol / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005453:Fluorescence; D006801:Humans
chemical_list: D000420:Albuterol