title: CTX and its desacetyl derivative (des-CTX): interaction with some representative beta-lactamases and their related pattern of resistance to newer selected clinical isolates.
abstract: In this study the kinetic features of cefotaxime (CTX) and desacetyl-cefotaxime towards several representative beta-lactamases were investigated. Desacetyl-CTX was more stable to hydrolysis in comparison with cefotaxime for all the investigated enzymes. However, a cephalosporinase produced in Acinetobacter was progressively inactivated by both CTX and des-CTX. After prolonged incubation, dialysis partially restored the enzyme activity. Finally, both compounds were tested against selected resistant strains. It is concluded that des-CTX, because of either poor hydrolysis or prolonged half-life in body fluids, could contribute in vivo to the good antimicrobial properties of cefotaxime.
authors.affiliation: Department of Sciences and Biomedical Technologies, Faculty of Medicine and Surgery, University of Aquila, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Amicosante
authors.forename: M
authors.initials: M
authors.lastname: Perilli
authors.forename: A
authors.initials: A
authors.lastname: Felici
authors.forename: B
authors.initials: B
authors.lastname: Segatore
authors.forename: N
authors.initials: N
authors.lastname: Franceschini
authors.forename: F
authors.initials: F
authors.lastname: Basani
authors.forename: M
authors.initials: M
authors.lastname: Di Girolamo
authors.forename: A
authors.initials: A
authors.lastname: Oratore
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002439:Cefotaxime / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D004755:Enterobacteriaceae / Q000187:drug effects; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D065093:beta-Lactamase Inhibitors
chemical_list: D000900:Anti-Bacterial Agents; D065093:beta-Lactamase Inhibitors; C029281:desacetylcefotaxime; D002439:Cefotaxime