Preservation of the quantity of lactobacilli in samples from the oral cavity.
Transport media for the preservation of streptococci from oral samples have been described, but a suitable transport medium for the preservation of lactobacilli in oral samples is yet to be established. In this study MRS, ROGOSA, and modifications of these media were evaluated as transport media for the quantitative preservation of oral lactobacilli. Results indicated a survival of ca. 100 per cent oral lactobacilli for both media when stored at 4 degrees C for up to 72 h. A decrease in the survival rate was obtained upon storage at -196 degrees C and growth occurred upon storage at 20 degrees C. It was evident throughout that carbohydrate-free modifications of both media best supported the quantitative survival of oral lactobacili. Carbohydrate-free modifications of both MRS and ROGOSA are therefore recommended as preservative transport media for the quantity of oral lactobacilli in oral samples for limited time periods.
Botha
S J
SJ
Centre for Stomatological Research, University of Pretoria.
D003470:Culture Media; D003773:Dental Plaque / Q000382:microbiology; D007778:Lactobacillus; D013048:Specimen Handling
D003470:Culture Media