Activation and inhibition of rat liver L-threonine dehydrogenase.
We have studied some characteristics of mitochondrial rat liver L-threonine dehydrogenase during freezing: the enzyme shows a net increase in activity and a "shift" in the pH optimum after storage at -20 degrees C of mitochondrial preparations. An unknown effector, which inhibited significantly the dehydrogenase activity, was isolated from the same preparations kept for 7-10 days at -20 degrees C. Some properties of the inhibitor have been determined.
Pagani
R
R
Istituto di Chimica Biologica, Università, Siena.
Guerranti
R
R
Leoncini
R
R
Marinello
E
E
D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D015925:Cryopreservation; D004355:Drug Stability; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology*; D051381:Rats; D013912:Threonine
D004791:Enzyme Inhibitors; D013912:Threonine; D000429:Alcohol Oxidoreductases; C023159:L-threonine 3-dehydrogenase