title: The immunoglobulin M heavy chain constant region gene of the channel catfish, Ictalurus punctatus: an unusual mRNA splice pattern produces the membrane form of the molecule.
abstract: The immunoglobulin (IgM) heavy chain constant region gene of the channel catfish, Ictalurus punctatus, has been cloned and characterized. The gene contains four constant region domain-encoding exons (CH1 to CH4) expressed in the secreted form of the immunoglobulin, and two exons encoding the transmembrane (TM) domain utilized in the lymphocyte membrane receptor form of the immunoglobulin. The sequence of a cDNA clone encoding the 3' region of the message for the membrane receptor form of the mu chain indicates that the TM1 exon is spliced directly to the CH3 exon, and not into a site within the CH4 exon, as occurs in the mammals, a shark and an amphibian. This unusual pattern of splicing, which produces a membrane heavy chain that is characteristically smaller than the secreted heavy chain, may be common to all teleost fish.
authors.affiliation: Basel Institute for Immunology, Switzerland.
authors.forename: M R
authors.initials: MR
authors.lastname: Wilson
authors.forename: A
authors.initials: A
authors.lastname: Marcuz
authors.forename: F
authors.initials: F
authors.lastname: van Ginkel
authors.forename: N W
authors.initials: NW
authors.lastname: Miller
authors.forename: L W
authors.initials: LW
authors.lastname: Clem
authors.forename: D
authors.initials: D
authors.lastname: Middleton
authors.forename: G W
authors.initials: GW
authors.lastname: Warr
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002397:Catfishes / Q000235:genetics*; D003001:Cloning, Molecular; D005091:Exons; D005803:Genes, Immunoglobulin; D007059:Ictaluridae / Q000235:genetics*; D007127:Immunoglobulin Constant Regions / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007075:Immunoglobulin M / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping
chemical_list: D007127:Immunoglobulin Constant Regions; D007143:Immunoglobulin Heavy Chains; D007075:Immunoglobulin M; D008565:Membrane Proteins; D012333:RNA, Messenger