Synchronous fluorescence spectrophotometry of benzo[a]pyrene diol epoxide-DNA adducts in workers exposed to polycyclic aromatic hydrocarbons.
Synchronous fluorescence spectrophotometry (SFS) for benzo[a]pyrene diol epoxide (BPDE)-DNA adducts detects adducts in lymphocyte DNA in some but not all individuals exposed occupationally to polycyclic aromatic hydrocarbons (PAHs). Individual differences in exposure, activation and/or DNA repair can be detected by SFS. Several in vitro and in vivo studies are currently being done to clarify the specificity and usability of SFS as a biomonitoring method for PAH exposure in the work environment.
Vähäkangas
K
K
Department of Pharmacology and Toxicology, University of Oulu, Finland.
Yrjänheikki
E
E
D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000032:analysis*; D004247:DNA / Q000032:analysis*; D018736:DNA Adducts; D004784:Environmental Monitoring; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry*; D008669:Metallurgy; D016273:Occupational Exposure; D013050:Spectrometry, Fluorescence / Q000379:methods
D018736:DNA Adducts; C043931:benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNA; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D004247:DNA