title: Neuron-specific and state-specific differences in calcium homeostasis regulate the generation and degeneration of neuronal architecture.
abstract: Many stimuli (e.g., neurotransmitters and electrical activity) regulate neuromorphogenesis by changing intracellular calcium. The ionophore A23187 was employed as a receptor-independent method to investigate neuronal calcium homeostasis. Distinctive neuron-specific (B5 versus B19) and state-specific (growing versus non-growing) differences in calcium homeostasis were observed in cultured identified Helisoma neurons. Fura-2 studies revealed that A23187 induced a transient rise in intracellular calcium in growing neurons B5 but a sustained rise in growing neurons B19. In stable-state (non-growing) cells A23187 evoked only a transient calcium rise. Both neuron-specific and state-specific differences in calcium homeostasis were dependent on extracellular sodium. Morphological studies also indicated that such differences in calcium-regulatory capacity can have profound consequences on the generation and degeneration of neuronal architecture.
authors.affiliation: Department of Anatomy and Neurobiology, Colorado State University, Fort Collins 80523.
authors.forename: L R
authors.initials: LR
authors.lastname: Mills
authors.forename: S B
authors.initials: SB
authors.lastname: Kater
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000502:physiology*; D002470:Cell Survival; D002478:Cells, Cultured; D006706:Homeostasis / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D012908:Snails; D012964:Sodium / Q000494:pharmacology
chemical_list: D000001:Calcimycin; D012964:Sodium; D002118:Calcium