title: Human neuronal cells in culture: from concepts to basic methodology.
abstract: The paper reviews some conceptual and methodological aspects of the tissue culture models which, during the past three decades, demonstrated a remarkable mimicry of many important structures and functions of the mammalian Central Nervous System (CNS) and related peripheral sensory and motor elements. Emphasis is placed on an original human neuronal tissue culture model obtained from selective CNS areas. The different cell types were identified and the neurotrophic interactions preliminary characterized. Neuropathological findings suggest hypothesis that can be fully tested using in vitro human models of affected cerebral specific areas.
authors.affiliation: Centro Dino Ferrari, Universitá di Milano.
authors.forename: V
authors.initials: V
authors.lastname: Silani
authors.forename: A
authors.initials: A
authors.lastname: Pizzuti
authors.forename: M F
authors.initials: MF
authors.lastname: Donato
authors.forename: A
authors.initials: A
authors.lastname: Falini
authors.forename: R
authors.initials: R
authors.lastname: Bassani
authors.forename: O
authors.initials: O
authors.lastname: Strada
authors.forename: R I
authors.initials: RI
authors.lastname: Causarano
authors.forename: D
authors.initials: D
authors.lastname: Mariani
authors.forename: R M
authors.initials: RM
authors.lastname: Villani
authors.forename: G
authors.initials: G
authors.lastname: Scarlato
mesh_terms: D001921:Brain / Q000166:cytology*; D002421:Caudate Nucleus / Q000166:cytology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology; D046508:Culture Techniques / Q000379:methods*; D005333:Fetus / Q000166:cytology; D006801:Humans; D007150:Immunohistochemistry; D009474:Neurons / Q000737:chemistry / Q000166:cytology*