title: Increased numbers of T lymphocytes with gamma delta-positive antigen receptors in a subgroup of individuals with pulmonary sarcoidosis.
abstract: Individuals with sarcoidosis were evaluated for preferential usage of T cells with the gamma delta-positive (+) type of T cell antigen receptor. Compared with normal subjects (n = 19), the group with sarcoidosis had increased numbers of CD3+ alpha beta-negative (-) T cells in the blood (normal, 58 +/- 12 cells/microliters; sarcoid, 192 +/- 45 cells/microliters, P less than 0.05) and in the epithelial lining fluid of the lung (normal, 78 14 cells/microliters; sarcoid, 240 +/- 60 cells/microliters, P less than 0.04) and a concomitant elevated number of blood and lung CD3+ gamma delta+ T cells, owing to a striking increase in the number of CD3+ gamma delta+ T cells in a subgroup (7 of 20) of sarcoid individuals. The elevated numbers of sarcoid blood gamma delta+ T lymphocytes were mostly Ti gamma A+ and delta TCS1-, a pattern also seen in normal individuals, consistent with the majority of gamma delta+ T cells expressing one gamma-chain variable region, V gamma 9. The observation of an increase in the total gamma delta+ T cell numbers in a sarcoid subgroup suggests that various specific stimuli may trigger the expansion of different T cell subpopulations within different groups of individuals with sarcoidosis.
authors.affiliation: Pulmonary Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: B
authors.initials: B
authors.lastname: Balbi
authors.forename: D R
authors.initials: DR
authors.lastname: Moller
authors.forename: M
authors.initials: M
authors.lastname: Kirby
authors.forename: K J
authors.initials: KJ
authors.lastname: Holroyd
authors.forename: R G
authors.initials: RG
authors.lastname: Crystal
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006801:Humans; D008168:Lung / Q000276:immunology; D008171:Lung Diseases / Q000276:immunology*; D046911:Macromolecular Substances; D008297:Male; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D012507:Sarcoidosis / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D013861:Thiocyanates
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D005452:Fluoresceins; D005456:Fluorescent Dyes; D046911:Macromolecular Substances; D011948:Receptors, Antigen, T-Cell; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate