title: Characterization of murine liver-derived inhibitory protein.
abstract: Murine liver-derived inhibitory protein (LIP) capable of inhibiting human lymphocyte proliferation was highly purified from liver extract. Its molecular weight determined by gel filtration and SDS-PAGE was 105,000 and 38,400 respectively. LIP moved electrophoretically at the gamma-globulin region. Its activity in inhibiting lymphocyte proliferation was temperature-stable up to 60 degrees C, and pH-stable between 4 and 11. It was not cytotoxic to lymphocytes as shown in 51Cr-release experiments. The purified LIP possessed arginase activity.
authors.affiliation: Department of Medicine, Veterans General Hospital, Taiwan, ROC.
authors.forename: S R
authors.initials: SR
authors.lastname: Wang
authors.forename: M H
authors.initials: MH
authors.lastname: Huang
authors.forename: K L
authors.initials: KL
authors.lastname: Chang
authors.forename: C L
authors.initials: CL
authors.lastname: Yu
authors.forename: B N
authors.initials: BN
authors.lastname: Chiang
authors.forename: S H
authors.initials: SH
authors.lastname: Han
mesh_terms: D000818:Animals; D001119:Arginase / Q000378:metabolism; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000502:physiology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008813:Mice, Inbred ICR; D008970:Molecular Weight; D011489:Protein Denaturation; D011506:Proteins / Q000302:isolation & purification / Q000494:pharmacology / Q000502:physiology*
chemical_list: D011506:Proteins; D001119:Arginase