Immunochemical localization of the fraction-1 antigen, a virulence determinant of Yersinia pestis.
The distribution of Yersinia pestis Fraction-1 (F1) antigen was analyzed in cells grown at 28 degrees C and 37 degrees C. Fractionation of Y. pestis cells followed by analysis in SDS-polyacrylamide gel electrophoresis indicated that the mature form of the F1 antigen is localized in the extracellular matrix and in the cytoplasm. Localization of the F1 antigen was confirmed by immunoblots and a single peptide with a molecular weight of 17,000 daltons was recognized. Electron microscopy of Y. pestis cells labeled with colloidal gold-conjugated antibodies corroborated the extracellular matrix and cytoplasm dual location of the F1 antigen.
Abath
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Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Cidade Universitária, Recife.
Almeida
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Ferreira
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D000942:Antigens, Bacterial / Q000032:analysis*; D002458:Cell Fractionation; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D014774:Virulence; D015010:Yersinia pestis / Q000276:immunology* / Q000472:pathogenicity
D000942:Antigens, Bacterial