title: Determination of cow milk formula allergenicity in the rat model by in vitro mast cell triggering and in vivo IgE induction.
abstract: The allergenicity of cow milk formulas was assessed in vitro by their ability to trigger prelabelled 3H-serotonin release from normal rat mast cells sensitized passively with rat reaginic anti-cow milk protein antisera. It was found that enzymatically hydrolyzed milk formulas had 1,000 (BEBAHA) to over 100,000 (ALFARE) times lower mast cell-triggering capacities than a standard milk formula (NAN). In vivo, these hydrolyzed formulas induced 100 (BEBAHA) to 10,000 (ALFARE) times less IgE antibodies than the standard milk formula.
authors.affiliation: Nestlé Research Centre, Lausanne, Switzerland.
authors.forename: R
authors.initials: R
authors.lastname: Fritsché
authors.forename: M
authors.initials: M
authors.lastname: Bonzon
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D007073:Immunoglobulin E / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology*; D016269:Milk Hypersensitivity / Q000276:immunology*; D008894:Milk Proteins / Q000276:immunology*; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000276:immunology
chemical_list: D008894:Milk Proteins; D012701:Serotonin; D007073:Immunoglobulin E