Evidence for two structural genes for alkaline phosphatase in Bacillus subtilis.
Two secreted alkaline phosphatase proteins were purified from cultures of Bacillus subtilis JH646MS. The two proteins showed slight differences in subunit molecular weight, substrate specificity, and charge characteristics. A total of 62% of the first 22 amino-terminal amino acids were identical. Both sequences showed conservation of structural features identified in Escherichia coli and human alkaline phosphatases. One alkaline phosphatase was a monomer and the other was a dimer. Southern analysis of genomic DNA with degenerative oligomers based on the amino acid sequences suggest that there are two structural genes for alkaline phosphatase in the genome of B. subtilis.
Hulett
F M
FM
Laboratory for Molecular Biology, University of Illinois, Chicago 60680.
Bookstein
C
C
Jensen
K
K
D000469:Alkaline Phosphatase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D002852:Chromatography, Ion Exchange; D005798:Genes, Bacterial; D006801:Humans; D007527:Isoenzymes / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D012689:Sequence Homology, Nucleic Acid
D007527:Isoenzymes; D000469:Alkaline Phosphatase