Different configurational states of beta-amyloid and their distributions relative to plaques and tangles in Alzheimer disease.
Antibodies have been raised against synthetic peptides corresponding to different parts of the beta-amyloid sequence. These antibodies stain different kinds of amyloid distributions in the hippocampal formation in Alzheimer disease, suggesting the existence of different states of aggregation and/or folding of beta-amyloid molecules. An antibody directed against the middle region of beta-amyloid stained mostly amyloid plaques without cores, whereas an antibody directed against the carboxyl-terminal region of beta-amyloid stained only amyloid plaques with cores. An antiserum directed against the amino terminus of beta-amyloid stained numerous tangle-bearing cells and bodies, as well as the neuritic component of plaques and neuropil threads. These antibodies, in conjunction with anti-tau antibodies, were used to demonstrate a close spatial relationship between amyloid deposits and neurofibrillary tangles.
Spillantini
M G
MG
Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom.
Goedert
M
M
Jakes
R
R
Klug
A
A
D000544:Alzheimer Disease / Q000473:pathology*; D000595:Amino Acid Sequence; D000682:Amyloid / Q000032:analysis* / Q000096:biosynthesis; D016229:Amyloid beta-Peptides; D000906:Antibodies; D000911:Antibodies, Monoclonal; D001921:Brain / Q000473:pathology*; D006624:Hippocampus / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D008869:Microtubule-Associated Proteins / Q000032:analysis; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000032:analysis; D011712:Pyramidal Tracts / Q000473:pathology; D016875:tau Proteins
D000682:Amyloid; D016229:Amyloid beta-Peptides; D000906:Antibodies; D000911:Antibodies, Monoclonal; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D016875:tau Proteins