Studies on immunomodulatory properties of isoniazid. IV. Effect of isoniazid on T lymphocyte activation by phorbol ester tumor promoter.
Human peripheral blood T cells or continuous T cell lines were treated with phorbol myristate (PMA), a direct protein kinase C activator. The effect of isoniazid on PMA-treated cells was investigated. It was found that low doses of isoniazid augmented proliferation of T cells treated with PMA or stimulated with PMA and phytohemagglutinin. Similar results were shown in the Jurkat cell line. There was no influence of isoniazid upon proliferation of PMA-treated HT-2 cells, interleukin 2-dependent cell line. The obtained results suggest that, at least one target mechanism of isoniazid is located in T cell activation pathway after protein kinase C activation.
Kucharz
E J
EJ
Department of Medicine, Medical College of Wisconsin, Milwaukee.
Sierakowski
S J
SJ
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D002460:Cell Line; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D010835:Phytohemagglutinins / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D010835:Phytohemagglutinins; D013755:Tetradecanoylphorbol Acetate; D007538:Isoniazid