Identification of a low-copy-number mutation within the pUB110 replicon and its effect on plasmid stability in Bacillus subtilis.
A mutation (cop1) within the minimal replicon of plasmid pUB110, reducing its copy number from 48 +/- 2 to 11 +/- 2 in a Bacillus subtilis host, was isolated. This mutation is a G----T transversion within the translation control region of the replication initiation gene (repU). The cop1 mutation, when present in the recombinant plasmid, increases both its structural and segregational stability.
Leonhardt
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Max-Planck-Institut für Molekulare Genetik, Berlin, Germany.
D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D012093:Replicon
D012333:RNA, Messenger