Complementation of the protein transport defect of an Escherichia coli secY mutant (secY24) by Bacillus subtilis secY homologue.
Bacillus subtilis SecY homologue shares 41.3% homology with that of E. coli and remarkably higher homologous regions (more than 80%) are present in the four cytoplasmic regions [(1990) J. Biochem. 107, 603-607]. Based on the formation of the mature form of OmpA in E. coli, we have shown that the protein transport defect of the E. coli secY mutant (secY24) is complemented by the gene product from the B. subtilis secY homologue, which is expressed under the lac promoter control. However, B. subtilis SecY could not restore growth of the E. coli mutant at nonpermissive temperature.
Nakamura
K
K
Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
Takamatsu
H
H
Akiyama
Y
Y
Ito
K
K
Yamane
K
K
D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D005838:Genotype; D009154:Mutation; D009876:Operon; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D000069816:SEC Translocation Channels; D012689:Sequence Homology, Nucleic Acid
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D000069816:SEC Translocation Channels; C057841:SecY protein, E coli