[Various aspects of protoplast production in Streptococcus lactis].
The authors studied the effect of some factors, including the conditions of preincubation, the action of 2-mercaptoethanol, EDTA, alpha-amylase, on protoplast production in four strains of Streptococcus lactis caused by lysozyme. The strains differed in the nisin-producing activity and in the structure of the cell walls that were not affected with lysozyme without either preincubation in 2-mercaptoethanol or in a salt medium with minimal inhibitory concentrations of DL-threonine. EDTA and alpha-amylase increased the lysozyme effect. Among seven buffer systems studied the most favourable for protoplast production in S. lactis is the ammonia-citric buffer with EDTA, and the best regeneration medium is the agar salt medium to which, depending on the strain, either glucose or sucrose should be added as a stabilizer.
Stoianova
L G
LG
Egorov
N S
NS
D002021:Buffers; D003470:Culture Media; D004492:Edetic Acid / Q000494:pharmacology; D013294:Lactococcus lactis / Q000187:drug effects / Q000254:growth & development / Q000648:ultrastructure*; D008623:Mercaptoethanol / Q000494:pharmacology; D009994:Osmolar Concentration; D011523:Protoplasts / Q000187:drug effects / Q000648:ultrastructure*; D013912:Threonine / Q000494:pharmacology; D000516:alpha-Amylases / Q000494:pharmacology
D002021:Buffers; D003470:Culture Media; D013912:Threonine; D008623:Mercaptoethanol; D004492:Edetic Acid; D000516:alpha-Amylases
Nekotorye aspekty polucheniia protoplastov u Streptococcus lactis]