Studies of human platelet ultrastructure and quantitative analyses of membrane glycoprotein IIB/IIIA in shear stress-induced platelet quick release reaction.
The ultrastructural characteristics of platelets and the quantitative analysis of membrane glycoprotein (GP) IIb/IIIa quick-release reaction induced by high shear stress (HSS) were studied with transmission electron microscope and immunocolloidal gold staining. The results showed that platelets were activated and aggregated by HSS (50 dynes/cm2 or 100 dynes/cm2). Besides, HSS also caused a special platelet quick-release reaction which was called granule-membrane-fused lytic release (GFLR). GFLR differed from releases I and II which were induced by ADP and thrombin respectively. Granules were released quickly and lysis occurred when all granules had been discharged in GFLR. GFLR is a special form of platelet quick release induced by HSS and is called release III by the authors. In the process of platelet activation and lysis, the gold particles of GPIIb/IIIa were increasingly condensed. The possible reasons of increased surface expression of GPIIb/IIIa stimulated by HSS are also discussed.
Song
M
M
Department of Histology and Embryology, First Military Medical University, Guangzhou.
Li
J
J
Yang
F C
FC
D001792:Blood Platelets / Q000032:analysis / Q000648:ultrastructure*; D006801:Humans; D007150:Immunohistochemistry; D010974:Platelet Aggregation; D010980:Platelet Membrane Glycoproteins / Q000032:analysis*; D013314:Stress, Mechanical
D010980:Platelet Membrane Glycoproteins