Oligonucleotides with pyrene fluorophore at the sugar fragment: synthesis and properties in binding to complementary polynucleotide.
Oligonucleotides with 1-pyrenylmethyl substituent at the designated sugar residue were synthesized by using 5'-dimethoxytrityl 2'-(1-pyrenylmethyl)uridine 3'-phosphorobisdiethylamidite 1. It was shown that the pyrene-oligonucleotides have enhanced affinity in binding to the complementary polynucleotide sequence. The fluorescence yield and life time of the pyrene-oligonucleotide was drastically enhanced when bound by the complementary polynucleotide.
Yamana
K
K
Department of Applied Chemistry, Himeji Institute of Technology, Japan.
Gokota
T
T
Ohashi
Y
Y
Ozaki
H
H
Kitamura
M
M
Nakano
H
H
Sangen
O
O
Shimidzu
T
T
D001483:Base Sequence; D002241:Carbohydrates / Q000737:chemistry; D005453:Fluorescence; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D011721:Pyrenes / Q000737:chemistry*
D002241:Carbohydrates; D009841:Oligonucleotides; D011721:Pyrenes