[Histological study of the lymph nodes of cattle, before and after antigenic stimulation. 2. Immunocytochemistry and electron microscopy].
A histological and cytological study of stimulated and non-stimulated bovine lymph nodes was performed using immunocytochemistry and electron-microscopic techniques. Immunohistochemical staining using polyclonal antibodies revealed cytoplasmic immunoglobulins producing lymphoid cells. A protein S100 specific serum evidenced antigen presenting cells, macrophages and endothelial cells. In order to identify T lymphocytes, two monoclonal antibodies (M1 and M12) were used. The electron-microscopic study confirmed the localization and identification of different lymphoid and non-lymphoid cells of the lymph node. The techniques used in the present work seem to be suitable for investigating bovine lymph nodes and those with pathologic alterations.
Femenia
F
F
Laboratoire d'Histologie et Anatomie Pathologique, INRA, Ecole Nationale Vétérinaire, Maisons Alfort, France.
Alogninouwa
T
T
Perre
J
J
Parodi
A L
AL
D000818:Animals; D002417:Cattle / Q000033:anatomy & histology*; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000648:ultrastructure*; D008854:Microscopy, Electron
Etude histologique, du ganglion lymphatique du bovin, avant et après stimulation antigénique: 2. Immunocytochimie et Microscopie électronique.