[Changes in protein fractions of milk obtained from cows with mastitis caused by Staphylococcus aureus. Preliminary studies].
The effect was studied of cow udder infection with S. aureus on changes of milk proteins. The tested milk was derived from two cows of the black-white lowland breed aged 5 years in the second stage of lactation. Proteins soluble and insoluble at pH 4.6 were characterized by means of starch-urea and polyacrylamide gel electrophoresis. The solution of nitrogen compound reacting with ninhydrin and soluble at pH 4.6 was separated by gel filtration, thin-layer chromatography and high-voltage paper electrophoresis, thin-layer chromatography and high-voltage paper electrophoresis. In the milk of the infected cow degradation of alpha s, beta and kappa casein, and increased content of gamma immunoglobulin. In whey proteins a rise was observed of serum albumin content and a decrease of beta-lactoglobulin and alpha lactoalbumin. In the solution after precipitation of casein at pH 4.6 in the milk of the infected cow a greater number of fractions reacting with ninhydrin was found. The observed range of degradation changes in milk protein in the infected cow (S. aureus) suggests that it has a negative effect on the technological, nutritional and hygienic value of milk.
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Katedra Połoznictwa Wydziału Weterynaryjnego Akademii Rolniczo Technicznej w Olsztynie.
D000418:Albumins / Q000032:analysis / Q000378:metabolism; D000818:Animals; D002364:Caseins / Q000032:analysis / Q000378:metabolism; D002417:Cattle; D002855:Chromatography, Thin Layer / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D007136:Immunoglobulins / Q000032:analysis / Q000378:metabolism; D007782:Lactoglobulins / Q000032:analysis / Q000378:metabolism; D008414:Mastitis, Bovine / Q000378:metabolism*; D008894:Milk Proteins / Q000032:analysis / Q000378:metabolism*; D013203:Staphylococcal Infections / Q000378:metabolism*; D013211:Staphylococcus aureus
D000418:Albumins; D002364:Caseins; D007136:Immunoglobulins; D007782:Lactoglobulins; D008894:Milk Proteins
Zmiany we frakcjach blałkowych mleka pochodzacego od krowy z zapaleniem wymienia wywołanym przez gronkowca złocistego. Badania wstepne.