Yeast artificial chromosomes: tools for mapping and analysis of complex genomes.
Libraries of yeast artificial chromosomes (YACs) are representative of complex genomes, and typical YACs contain single fragments of DNA that are up to a megabase or more in size, are stable during growth, and are faithful to genomic DNA. Such YACs may permit (1) the use of complete gene units for a number of research and medical purposes; and (2) the assembly of chromosome-sized contigs in a single map that unifies genetic and physical data.
Schlessinger
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Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110.
D000818:Animals; D002107:Caenorhabditis / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods*; D015825:Chromosomes, Fungal / Q000648:ultrastructure; D003001:Cloning, Molecular; D004331:Drosophila melanogaster / Q000235:genetics; D005786:Gene Expression Regulation; D030342:Genetic Diseases, Inborn / Q000235:genetics; D005822:Genetic Vectors; D015894:Genome, Human; D015698:Genomic Library; D006801:Humans; D012441:Saccharomyces cerevisiae / Q000235:genetics*