Structural characterization of a rice actin gene.
We have isolated and sequenced a full-length cDNA clone containing information for the rice actin gene RAc1. Transcript terminus mapping and sequence alignment between the RAc1 cDNA clone and a previously isolated RAc1 genomic clone were used to determine the structure of the RAc1 gene. This allowed us to make the first complete structural characterization of a plant actin gene. The analysis revealed the presence of a 5'-noncoding exon, separated by an intron, from the first translated exon of the RAc1 gene. This is one of the few reported cases of a plant gene containing such a 5'-noncoding exon. Sequence comparison between the previously isolated plant actin genes suggests that such an exon may be a common feature of plant actin gene structure. The present study also confirms that the rice actin gene family is composed of at least eight unique members.
McElroy
D
D
Field of Botany, Cornell University, Ithaca, NY 14853.
Rothenberg
M
M
Wu
R
R
D000199:Actins / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D017343:Genes, Plant; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012275:Oryza / Q000235:genetics*; D011544:Pseudogenes; D051381:Rats; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D000199:Actins; D015345:Oligonucleotide Probes