Xylan degradation by the thermophile Clostridium stercorarium: cloning and expression of xylanase, beta-D-xylosidase, and alpha-L-arabinofuranosidase genes in Escherichia coli.
Seven genes related to arabinoxylan degradation were isolated from a genomic library of the thermophilic bacterium Clostridium stercorarium. The cloned genes include a xylanase gene (xynA), two beta-D-xylosidase genes (bx1A and bx1B), two alpha-L-arabinofuranosidase genes (arfA and arfB), and two genes (celW and celX) encoding enzymes termed celloxylanases, which hydrolyze both xylans and beta-D-cellobiosides. The genes xynA, celX, and bxlB were found to encode the major xylanolytic enzyme activities induced by growth of C. stercorarium on xylan.
Schwarz
W H
WH
Institute for Microbiology, Technical University Munich, Federal Republic of Germany.
Adelsberger
H
H
Jauris
S
S
Hertel
C
C
Funk
B
B
Staudenbauer
W L
WL
D003001:Cloning, Molecular; D003013:Clostridium / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D004274:DNA, Recombinant / Q000032:analysis*; D004926:Escherichia coli / Q000235:genetics*; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000235:genetics; D011134:Polysaccharides / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D013379:Substrate Specificity; D043325:Xylan Endo-1,3-beta-Xylosidase; D014990:Xylans / Q000378:metabolism*; D014995:Xylosidases / Q000096:biosynthesis / Q000235:genetics
D004274:DNA, Recombinant; D011134:Polysaccharides; D011994:Recombinant Proteins; D014990:Xylans; D006026:Glycoside Hydrolases; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase; C026579:exo-1,4-beta-D-xylosidase; C019713:alpha-N-arabinofuranosidase