Studies on the biosynthesis of iturin, an antibiotic of Bacillus subtilis, and a lipopeptide containing beta-hydroxy fatty acids.
The biosynthesis of iturin, an antibiotic containing a beta-amino fatty acid, was studied by incubating Bacillus subtilis in the presence of various 14C-labelled precursors. Sodium acetate or palmitic acid were incorporated into the beta-amino acids of iturin. Among the alpha-amino acids (asparagine, glutamine, serine, proline and tyrosine) in the peptidic part of iturin, asparagine appears to be the best precursor. In the presence of sodium [14C]acetate or [14C]asparagine, there was a synthesis of radioactive compound (compound X) before the synthesis of radioactive iturin. Compound X contained asparagine and/or aspartic acid, glutamine and/or glutamic acid and beta-hydroxy fatty acids.
Besson
F
F
Laboratoire de Biochimie Microbienne, Université Claude Bernard, Villeurbanne, France.
Hourdou
M L
ML
Michel
G
G
D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001216:Asparagine / Q000378:metabolism; D001412:Bacillus subtilis / Q000187:drug effects / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002701:Chloramphenicol / Q000494:pharmacology; D006880:Hydroxy Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010455:Peptides; D010456:Peptides, Cyclic / Q000096:biosynthesis
D000085:Acetates; D000900:Anti-Bacterial Agents; D002250:Carbon Radioisotopes; D006880:Hydroxy Acids; D010455:Peptides; D010456:Peptides, Cyclic; C052532:iturin D; C052538:iturin E; C013579:iturin A; C033468:iturin C; D002701:Chloramphenicol; D001216:Asparagine; D019342:Acetic Acid