On-line recovery of trimeprazine enantiomers following chiral separation by reversed-phase high-performance liquid chromatography using a beta-cyclodextrin-containing mobile phase.
A procedure is described which allows the on-line recovery of enantiomers following semi-preparative chiral separation by RP-HPLC using beta-cyclodextrin in the mobile phase. By this method, the phenothiazine antihistamine trimeprazine (I) was resolved into its antipodes at greater than 95% optical purity at a throughput of more than 1 mg of each enantiomer per hour, using 4 mm i.d. columns. The recovered trimeprazine was found to be free from cyclodextrin.
Cooper
A D
AD
School of Pharmacy and Pharmacology, University of Bath, UK.
Jefferies
T M
TM
D002851:Chromatography, High Pressure Liquid; D003505:Cyclodextrins / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D014291:Trimeprazine / Q000032:analysis*; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D047392:beta-Cyclodextrins; D014291:Trimeprazine; C031215:betadex