Rapid determination of beta-aminoisobutyric acid by reversed-phase high-performance liquid chromatography.
For the determination of beta-aminoisobutyric acid (BAIBA) in urine samples in which the beta-alanine concentrations are higher than those of BAIBA, the resolution between these two amino acids, separated by reversed-phase liquid chromatography on an octadecylsilane column, was optimized. The chromatographic analysis included precolumn derivatization of amino acids with o-phthalaldehyde, followed by a 15-min isocratic elution and detection at 340 nm. Because of its simplicity, this method should be useful for monitoring urinary excretion of BAIBA.
Ladrón de Guevara
O
O
Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico, D.F.
Cortinas de Nava
C
C
Padilla
P
P
Espinosa
J
J
Cebrian
M
M
García
L
L
D000621:Aminoisobutyric Acids / Q000032:analysis* / Q000302:isolation & purification / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007202:Indicators and Reagents; D013056:Spectrophotometry, Ultraviolet; D015091:beta-Alanine / Q000652:urine
D000621:Aminoisobutyric Acids; D007202:Indicators and Reagents; D015091:beta-Alanine; C033435:3-aminoisobutyric acid