title: Effect of prolactin on phosphatidylcholine hydrolysis via phospholipase C in isolated adrenocortical cells of guinea pig.
abstract: Prolactin significantly increased the rate of incorporation of [3H]choline into phosphorylcholine (PRC) in a purified suspension of guinea pig adrenocortical cells. The rate of phosphatidylcholine (PTC) labelling and cellular PTC content did not change. In cells prelabelled with [3H]choline or CDP [14C]choline, prolactin diminished the rate of reduction of the radioactive PRC pool after 60-90 min incubation without any change in the rate of PTC biosynthesis. Taken together, these findings suggest that prolactin stimulates the hydrolysis of PTC by phospholipase C into PRC and diacylglycerol. The significance of this effect as part of the mechanism of action of prolactin on adrenocortical cells is discussed.
authors.affiliation: Institute of Endocrinology and Metabolism, Kiev, USSR.
authors.lastname: Sautin YuY
authors.forename: N D
authors.initials: ND
authors.lastname: Tronko
authors.forename: A S
authors.initials: AS
authors.lastname: Mikosha
mesh_terms: D000302:Adrenal Cortex / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D004075:Diglycerides / Q000378:metabolism; D006168:Guinea Pigs; D006868:Hydrolysis; D007700:Kinetics; D010713:Phosphatidylcholines / Q000378:metabolism*; D010767:Phosphorylcholine / Q000378:metabolism; D011388:Prolactin / Q000502:physiology*; D010738:Type C Phospholipases / Q000378:metabolism*
chemical_list: D004075:Diglycerides; D010713:Phosphatidylcholines; D010767:Phosphorylcholine; D011388:Prolactin; D010738:Type C Phospholipases