Platelet-fibrin interaction in the suspension and under flow conditions.
Interactions between platelets and fibrin are important in hemostasis but often confused with platelet-fibrinogen interactions. A stirred mixture of solubilized fibrin and washed platelets at neutral pH range showed drastic reduction in turbidity and concomitant platelet adhesion onto newly formed fibrin strands. This platelet-fibrin interaction did not require platelet activation nor did it cause platelet aggregation. A device consisting of a parallel-plate flow chamber mounted on a fluorescence microscope has been constructed to allow direct visualization and recording of platelet-fibrin interaction under flow conditions. Platelets in whole blood adhered to the fibrin-coated portion but not to the uncoated portion of the flow chamber. Slow motion playback of video tapes indicated that the adhesion phenomenon was a dynamic process that involved attaching, detaching, relocation and transient contact. The fibrin coating influenced platelet adhesion both by increasing the number of cells making short-term attachments to the surface and by increasing the duration of cells attached to the surface. These observations provided basic characteristics of platelet-fibrin interaction.
Jen
C J
CJ
Department of Physiology, National Cheng-Kung University, Tainan, Taiwan, ROC.
Hu
S J
SJ
Wu
H J
HJ
Lin
T S
TS
Mao
C W
CW
D001792:Blood Platelets / Q000187:drug effects / Q000502:physiology*; D005337:Fibrin / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology
D010975:Platelet Aggregation Inhibitors; D005337:Fibrin