13-cis retinoyl-beta-glucuronide in lacrimal gland fluid of rabbits treated with 13-cis retinoic acid.
When rabbits and humans are treated with orally administered 13-cis retinoic acid, the retinoic acid and a more polar retinoid metabolite appear in tears and lacrimal gland fluid. This study tested the hypothesis that this metabolite was a retinoyl-beta-glucuronide. Lacrimal gland fluid from rabbits treated with a pharmacologic dose of 13-cis retinoic acid was analyzed. Based on chromatographic retention time, absorbance maximum, and degradation to 13-cis retinoic acid by the enzyme beta-glucuronidase, it was shown that this retinoid metabolite is 13-cis retinoyl-beta-glucuronide. The 13-cis retinoyl-beta-glucuronide was also extracted from the lacrimal gland itself. It is concluded that 13-cis retinoyl-beta-glucuronide is a major metabolite of 13-cis retinoic acid in the rabbit and that this retinoid is secreted by the lacrimal gland.
Ubels
J L
JL
Department of Ophthalmology, Medical College of Wisconsin, Milwaukee.
Osgood
T B
TB
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005966:Glucuronidase / Q000378:metabolism; D007765:Lacrimal Apparatus / Q000187:drug effects / Q000378:metabolism*; D011817:Rabbits; D013666:Tears / Q000378:metabolism; D014212:Tretinoin / Q000008:administration & dosage* / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism
C026787:retinoyl glucuronide; D014212:Tretinoin; D005966:Glucuronidase