title: Trypanosoma cruzi: partial prevention of the natural infection of guinea pigs with a killed parasite vaccine.
abstract: Guinea pigs are natural reservoirs of Chagas' disease. Domestic breeding and local trade of these animals are common practices among andean communities in South America. Infection by Trypanosoma cruzi occurs when the animals live in triatomine-infested houses or yards. The preventive effect of a vaccine consisting of cultured T. cruzi killed by freezing and thawing plus saponin was tested both in mice and in the guinea pig ecosystem. Resistance against T. cruzi challenge in mice was improved by increasing the trypomastigote/epimastigote ratio in live attenuated vaccines but not in killed parasite vaccines. Although the killing of attenuated parasites sharply reduced their immunogenicity for mice, a protective effect against natural T. cruzi infection was detected in guinea pigs. A total of 88 guinea pigs were vaccinated in four intradermal sites on three occasions. Eighty controls received similar inoculations of culture medium plus saponin. All animals were kept in a triatomine-infested yard. Parasitemia was studied with the capillary microhematocrit method. After an exposure time averaging 4 months, natural T. cruzi infection occurred in 55% (44/80) of the controls and in 33% (29/88) of the vaccinated group (P less than 0.01). The number of highly parasitemic guinea pigs was also significantly decreased (6/80 vs 0/88, P less than 0.01). Thus, immunizing protocols which are only partially protective against artificial callenge with T. cruzi may nevertheless constrain the exchange of parasites between natural hosts and vectors.
authors.affiliation: Laboratorio de Patología Experimental, Facultad de Ciencias de la Salud, Universidad Nacional de Salta, Calle Buenos Aires, Argentina.
authors.forename: M A
authors.initials: MA
authors.lastname: Basombrio
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000517:prevention & control / Q000662:veterinary*; D004197:Disease Reservoirs; D005615:Freezing; D006168:Guinea Pigs / Q000469:parasitology*; D007303:Insect Vectors; D051379:Mice; D012376:Rodent Diseases / Q000517:prevention & control*; D014224:Triatoma; D014349:Trypanosoma cruzi / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary; D014613:Vaccines, Attenuated; D015164:Vaccines, Inactivated
chemical_list: D014613:Vaccines, Attenuated; D015164:Vaccines, Inactivated