title: A cytoplasmic factor involved in the expression of resistance to C8-ATC in Saccharomyces cerevisiae.
abstract: A dozen mutants of Saccharomyces cerevisiae, resistant to C8-ATC have been characterized. C8-ATC was previously established as a biologically toxic compound. Frequency of mutants (10(-7)) was typical for spontaneous mutations. One very stable mutant was characterized extensively. The genetical analysis revealed that resistance in this mutant was determined by single-gene mutation. The rho 0 cells, obtained by ethidium bromide (EB) mutagenesis of the resistant strain, were completely devoid of resistance. A large percentage of rho- cells, obtained by a moderate EB treatment of resistant cells were still able to express resistance to C8-ATC. Therefore we hypothesized that, in our particular strain, a cytoplasmic factor is involved in nuclear determination of resistance.
authors.affiliation: Wroclaw University, Department of Genetics, Poland.
authors.forename: M H
authors.initials: MH
authors.lastname: Misiewicz
authors.forename: Z
authors.initials: Z
authors.lastname: Kotylak
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D003593:Cytoplasm / Q000502:physiology*; D004352:Drug Resistance, Microbial / Q000235:genetics; D005285:Fermentation; D005690:Galactose / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism; D008928:Mitochondria / Q000502:physiology; D009154:Mutation; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development
chemical_list: D000935:Antifungal Agents; D000644:Quaternary Ammonium Compounds; D005947:Glucose; D005690:Galactose