title: The generation of concatemeric plasmid DNA in Bacillus subtilis as a consequence of bacteriophage SPP1 infection.
abstract: Bacteriophage SPP1 infection of Bacillus subtilis cells bearing plasmids induces the synthesis of multigenome-length plasmid molecules. Two independent pathways can account for this synthesis. In one of those, homology to the phage genome is required, whereas in the other such homology is not a prerequisite. In wild type cells both modes overlap. In dnaB(Ts), at non permissive temperature, or in recE polA strains the main concatemeric plasmid replication mode is the homology-dependent plasmid (hdp) mode. The rate of recombination-dependent concatemeric plasmid DNA synthesis is a consequence of a phage-plasmid interaction which leads to chimeric phage::plasmid DNA. The second mode, which is an homology-independent plasmid (hip) mode seems to be triggered upon the synthesis of a phage encoded product(s) (e.g. inactivation of the exonuclease V enzyme).
authors.affiliation: Max-Planck-Institut für Molekulare Genetik, Berlin, FRG.
authors.forename: A
authors.initials: A
authors.lastname: Bravo
authors.forename: J C
authors.initials: JC
authors.lastname: Alonso
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics* / Q000502:physiology; D015139:Blotting, Southern; D002678:Chimera / Q000235:genetics; D004269:DNA, Bacterial / Q000096:biosynthesis*; D005798:Genes, Bacterial; D005814:Genes, Viral; D010957:Plasmids; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature; D014161:Transduction, Genetic; D014779:Virus Replication
chemical_list: D004269:DNA, Bacterial