[The preservation of a DNA repair disorder in continuous-line fibroblasts obtained from gout patients].
DNA repair was explored in continuous cells withdrawn from gout patients. The data obtained were compared to those on primary cells (lymphocytes) from the same patients. Two continuous lines of fibroblasts obtained from the biopsy material of patients suffering from gout were examined for stability of reparation defects on long cell passage. The studies were made with 4 to 12 passages of patients' fibroblasts. The use of criteria reflecting certain stages of DNA repair (reparative synthesis of DNA, formation of induced DNA ruptures and their resynthesis during cell postincubation, reactivation and induced mutagenesis of measles vaccine virus in patients' cells) allowed confirmation of repair defect stability in gout patients' cells on their long passage. Based on the data on preservation of the repair defect on cell passage it is concluded that gout patients demonstrate the genetically determined impairment of the synthesis of DNA repair enzymes participating in the recovery of DNA impairments induced by UV radiation or UV mimetics.
Filippova
V G
VG
Chekova
V V
VV
L'vova
G N
GN
Vasil'eva
I M
IM
Sinel'shchikova
T A
TA
Gubitskaia
E G
EG
Balkarov
I M
IM
Zasukhina
G D
GD
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D001706:Biopsy; D002460:Cell Line; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology / Q000528:radiation effects; D004247:DNA / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D004249:DNA Damage / Q000187:drug effects / Q000528:radiation effects; D004260:DNA Repair; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology / Q000528:radiation effects; D005720:Gamma Rays; D015432:Glomerulonephritis, Membranoproliferative / Q000378:metabolism; D006073:Gout / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology / Q000528:radiation effects; D012867:Skin / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D014466:Ultraviolet Rays; D014616:Vaccinia virus / Q000187:drug effects / Q000528:radiation effects; D014775:Virus Activation / Q000187:drug effects / Q000528:radiation effects
D015112:4-Nitroquinoline-1-oxide; D004247:DNA
Sokhranenie narushemiia reparatsii DNK v perevivaemykh fibroblastakh, poluchennykh ot bol'nykh podagroĭ.