Comparative approach to the evaluation of hemoglobin A2 by two different methods: high-performance liquid chromatography and DE-52 microchromatography.
Hb A2 was determined in 477 subjects: 77 were affected by iron deficiency anemia, 172 were carriers of beta-thalassemia trait and 228 were normal controls. Hb A2 was determined by both DE-52 microchromatography and high-performance liquid chromatography (HPLC). The analysis of the data by linear regression demonstrated that the methods furnish overlapping results. Our findings show that HPLC is a rapid and easily reproduced method which allows a quantitative and qualitative discrimination of the various Hb fractions, making it a valid tool in screening programs for hemoglobinopathies.
Samperi
P
P
Cattedra di Ematologia Pediatrica, Università di Catania, Italia.
Testa
R
R
Mancuso
M
M
Schilirò
G
G
D000328:Adult; D000747:Anemia, Hypochromic / Q000097:blood*; D002648:Child; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D006580:Genetic Carrier Screening; D006441:Hemoglobin A / Q000032:analysis*; D006443:Hemoglobin A2 / Q000032:analysis*; D006801:Humans; D008297:Male; D012016:Reference Values; D013789:Thalassemia / Q000097:blood* / Q000235:genetics
D006441:Hemoglobin A; D006443:Hemoglobin A2