T-DNA presence and opine production in tumors of Picea abies (L.) Karst induced by Agrobacterium tumefaciens A281.
The hypervirulent Agrobacterium tumefaciens strain A281 formed frequent tumors (31%) on Picea abies (Norway spruce), an economically important tree species in Swedish forests. Three-month-old seedlings were inoculated and tumors were established that grew hormone-independently in culture. Tumors contained agropine and mannopine/mannopinic acid as determined by acid pH paper electrophoresis. In addition, DNA hybridization studies showed that the DNA from these tumor lines contained sequences homologous to Ti plasmid T-DNA, whereas wild-type spruce seedling DNA did not. These results suggest that Agrobacterium vectors can be used for gene transfer into this important forest species.
Hood
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Dept. of Forest Genetics, Swedish University of Agricultural Sciences, Uppsala.
Clapham
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DH
Ekberg
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Johannson
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T
D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D005822:Genetic Vectors; D010941:Plant Tumors / Q000382:microbiology*; D010944:Plants / Q000235:genetics; D010957:Plasmids; D015183:Restriction Mapping; D012231:Rhizobium / Q000235:genetics* / Q000472:pathogenicity; D014162:Transfection; D014197:Trees
D015342:DNA Probes; D004269:DNA, Bacterial; C036483:T-DNA