Tissue-specific expression directed by an Arabidopsis thaliana pre-ferredoxin promoter in transgenic tobacco plants.
We have isolated and analyzed a pre-ferredoxin gene from Arabidopsis thaliana. This gene encodes a 148 amino acid precursor protein including a chloroplast transit peptide of 52 residues. Southern analysis shows the presence of a single copy of this ferredoxin (Fd) gene in the A. thaliana genome. Its expression is tissue-specific and positively affected by light. Response times, both to dark and light conditions, are remarkably rapid. A chimeric gene consisting of a 1.2 kb Fd promoter fragment fused to the beta-glucuronidase reporter gene was transferred to tobacco. This fusion gene is expressed in a tissue-specific way; it shows high levels of expression in green leaves, as compared to root tissue.
Vorst
O
O
Department of Molecular Cell Biology, University of Utrecht, Netherlands.
van Dam
F
F
Oosterhoff-Teertstra
R
R
Smeekens
S
S
Weisbeek
P
P
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005288:Ferredoxins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005818:Genetic Engineering; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D010944:Plants / Q000235:genetics*; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism; D015183:Restriction Mapping; D014018:Tissue Distribution; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism
D005288:Ferredoxins; D010940:Plant Proteins; D011498:Protein Precursors; D004247:DNA