Purification and characterization of tumor invasion-inhibiting factors.
Tumor invasion-inhibiting factors were purified from bovine liver using an in vitro system for estimating the tumor invasion ability. The acid-ethanol extract of liver was subjected to ultrafiltration (Amicon PM10 membrane), and the fraction corresponding to the molecular weight range below 10,000 was further fractionated by ion-exchange (DEAE-Toyopearl), gel filtration (Bio-Gel P6), and reverse-phase (C18) chromatographies. Two types of active polypeptides with molecular weights of about 5,000 and 2,000 were purified and named IIF-1 and IIF-2, respectively. Both peptides inhibited tumor invasion with half-maximum concentrations of 2-6 ng/ml in vitro. The amino acid compositions of both peptides were determined.
Isoai
A
A
Research Center, Asahi Glass Co., Ltd., Yokohama.
Giga-Hama
Y
Y
Shinkai
K
K
Mukai
M
M
Akedo
H
H
Kumagai
H
H
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification*; D001685:Biological Factors / Q000302:isolation & purification*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D066298:In Vitro Techniques; D008099:Liver / Q000737:chemistry; D008970:Molecular Weight; D009362:Neoplasm Metastasis; D009374:Neoplasms, Experimental / Q000737:chemistry / Q000473:pathology*; D010455:Peptides / Q000302:isolation & purification; D051381:Rats; D014407:Tumor Cells, Cultured
D000596:Amino Acids; D000970:Antineoplastic Agents; D001685:Biological Factors; D010455:Peptides