title: Chromatin superstructure-dependent crosslinking with DNA of the histone H5 residues Thr1, His25 and His62.
abstract: The points of histone H5 interactions with DNA within nucleosomes and chromatin at different levels of compaction are delineated by identification of H5 amino acid residues that can be covalently bound to DNA. Three major crosslinkable points of H5 are His25, His62 (both within the globular part of the molecule), and N-terminal Thr1. His25 interacts with the terminal regions of nucleosomal DNA; His62 appears to bind more distal segments of the linker DNA. The His25-DNA crosslink predominates in the isolated mononucleosomes and persists throughout the chromatin condensation states studied, from extended oligonucleosomal chains to nuclei. His62 is the strongest crosslinking site in nuclei; in oligonucleosomes, the predominance of the His62-DNA crosslink requires the number of nucleosomes in the chain to be above some critical value. The Thr1-DNA crosslink is generated only in decondensed poly- or oligonucleosomes, but not in mononucleosomes. Thus, underlying the higher-order folding transitions of the nucleosomal chain is the restructuring of H5-DNA interactions.
authors.affiliation: W. A. Engelhardt Institute of Molecular Biology, Academy of Sciences of the U.S.S.R., Moscow.
authors.forename: A D
authors.initials: AD
authors.lastname: Mirzabekov
authors.forename: D V
authors.initials: DV
authors.lastname: Pruss
authors.forename: K K
authors.initials: KK
authors.lastname: Ebralidse
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000378:metabolism / Q000648:ultrastructure*; D003432:Cross-Linking Reagents; D004247:DNA / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism; D006639:Histidine; D006657:Histones / Q000097:blood*; D008969:Molecular Sequence Data; D009707:Nucleosomes / Q000378:metabolism / Q000648:ultrastructure; D010449:Peptide Mapping; D011485:Protein Binding; D013912:Threonine; D014357:Trypsin
chemical_list: D002843:Chromatin; D003432:Cross-Linking Reagents; D006657:Histones; D009707:Nucleosomes; D013912:Threonine; D006639:Histidine; D004247:DNA; D014357:Trypsin