title: [The genetic transfer, heredity and phenotypic expression of plasmid RP4::Mu cts genes in Bacillus cereus].
abstract: The transcipients were obtained in intrageneric matings of E.coli donor harbouring the plasmid PR4::Mu cts 62 with Bac. cereus GP7 recipient cells with the frequency 10(-9). The transcipient clone Bac. cereus 682 was selected having stably inherited and expressed the hybrid plasmid PR4::Mu cts 62 genes for antibiotic resistance and temperature sensitivity. Production of the bacteriophage Mu cts 62 particles was not registered in the bacillary transcipient cells. The plasmid RP4::Mu cts 62 genes were localized in the chromosome of Bac. cereus 682 transcipient by the blot-hybridization technique with 32P-labelled DNA of the bacteriophage Mu cts 62 and the plasmid PR4. The transcipient of Bac. cereus 682 has the donor properties and transfers the RP4::Mu cts 62 genes to recipient cells of Bac. cereus DSM 318 with the frequency of 10(-6)-10(-7). The expression and transfer of the gram-negative plasmid genes in gram-positive bacterial cells are discussed.
authors.forename: N V
authors.initials: NV
authors.lastname: Timakova
authors.forename: G I
authors.initials: GI
authors.lastname: Aleshkin
authors.forename: I V
authors.initials: IV
authors.lastname: Titova
authors.forename: M G
authors.initials: MG
authors.lastname: Goderdzishvili
authors.forename: V I
authors.initials: VI
authors.lastname: Zakharenko
authors.forename: G V
authors.initials: GV
authors.lastname: Brukhanskiĭ
authors.forename: O G
authors.initials: OG
authors.lastname: Loginova
authors.forename: S Ia
authors.initials: SIa
authors.lastname: Ditiatkin
authors.forename: B N
authors.initials: BN
authors.lastname: Il'iashenko
mesh_terms: D001409:Bacillus cereus / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D005819:Genetic Markers; D010641:Phenotype; D010957:Plasmids / Q000235:genetics*; D013045:Species Specificity; D014169:Transformation, Bacterial
chemical_list: D005819:Genetic Markers
vernacular_title: Geneticheskiĭ perenos, nasledovanie i fenotipicheskoe vyrazhenie genov plazmidy RP4::Mu cts v Bacillus cereus.