title: Isolation and characterization of Patnopecten mid-gut gland endo-beta-xylosidase active on peptidochondroitin sulfate.
abstract: An endo-beta-xylosidase acting on the linkage region of peptidochondroitin sulfate was isolated from the mid-gut gland of the mollusc Patnopecten and purified about 375-fold, using a combination of ammonium sulfate fractionation, gel filtration on Sephacryl S-200, and DEAE-Sephacel chromatography. The pH optimum and the isoelectric point of this enzyme were 4.0 and 7.0, respectively. The molecular weight, estimated by gel filtration through Sephacryl S-200, was 78,000. The purified enzyme was completely free from protease, exoglycosidases, sulfatase, and phosphatase. This enzyme hydrolyzed the xylosyl serine linkage of the linkage region of various glycosaminoglycans, that is chondroitin sulfate, dermatan sulfate and heparan sulfate, all possessing a very small peptide segment, but not proteoglycans. It was concluded that this endo-beta-xylosidase was involved in the catabolism of proteoglycans.
authors.affiliation: Department of Biochemistry, Hirosaki University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Takagaki
authors.forename: A
authors.initials: A
authors.lastname: Kon
authors.forename: H
authors.initials: H
authors.lastname: Kawasaki
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: S
authors.initials: S
authors.lastname: Tamura
authors.forename: M
authors.initials: M
authors.lastname: Endo
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002807:Chondroitin / Q000031:analogs & derivatives*; D002809:Chondroitin Sulfates / Q000378:metabolism*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002854:Chromatography, Paper; D004588:Electrophoresis, Cellulose Acetate; D006026:Glycoside Hydrolases / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D008974:Mollusca / Q000201:enzymology*; D014995:Xylosidases / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: C062473:peptidochondroitin sulfate; D002807:Chondroitin; D002809:Chondroitin Sulfates; D006026:Glycoside Hydrolases; D014995:Xylosidases; C056547:endo-beta-xylosidase