title: Overexpression of the CDC25 gene, an upstream element of the RAS/adenylyl cyclase pathway in Saccharomyces cerevisiae, allows immunological identification and characterization of its gene product.
abstract: The product of the START gene CDC25, an upstream element of the RAS/adenylyl cyclase pathway in Saccharomyces cerevisiae, was identified using specific antibodies raised against a chimeric beta-galactosidase/CDC25 protein. The CDC25 protein is poorly expressed and can be detected only when the CDC25 gene is overexpressed under the control of the galactose-inducible GAL1-10 strong promoter elements. It has a molecular weight of 180,000, is not glycosylated and is strongly associated with the particulate fraction. After deletion of residues 1255-1550 the protein is found in the soluble fraction.
authors.affiliation: Dipartimento di Fisologia e Biochimica Generali, Università degli Studi di Milano, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Vanoni
authors.forename: M
authors.initials: M
authors.lastname: Vavassori
authors.forename: G
authors.initials: G
authors.lastname: Frascotti
authors.forename: E
authors.initials: E
authors.lastname: Martegani
authors.forename: L
authors.initials: L
authors.lastname: Alberghina
mesh_terms: D000262:Adenylyl Cyclases / Q000235:genetics*; D000906:Antibodies; D018797:Cell Cycle Proteins; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D015151:Immunoblotting; D008970:Molecular Weight; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000276:immunology; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics / Q000276:immunology; D018631:ras Proteins; D020666:ras-GRF1
chemical_list: D000906:Antibodies; C542351:CDC25 protein, S cerevisiae; D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D020666:ras-GRF1; D001616:beta-Galactosidase; D018631:ras Proteins; D000262:Adenylyl Cyclases