title: Rapid determination of beta-aminoisobutyric acid by reversed-phase high-performance liquid chromatography.
abstract: For the determination of beta-aminoisobutyric acid (BAIBA) in urine samples in which the beta-alanine concentrations are higher than those of BAIBA, the resolution between these two amino acids, separated by reversed-phase liquid chromatography on an octadecylsilane column, was optimized. The chromatographic analysis included precolumn derivatization of amino acids with o-phthalaldehyde, followed by a 15-min isocratic elution and detection at 340 nm. Because of its simplicity, this method should be useful for monitoring urinary excretion of BAIBA.
authors.affiliation: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico, D.F.
authors.forename: O
authors.initials: O
authors.lastname: Ladrón de Guevara
authors.forename: C
authors.initials: C
authors.lastname: Cortinas de Nava
authors.forename: P
authors.initials: P
authors.lastname: Padilla
authors.forename: J
authors.initials: J
authors.lastname: Espinosa
authors.forename: M
authors.initials: M
authors.lastname: Cebrian
authors.forename: L
authors.initials: L
authors.lastname: García
mesh_terms: D000621:Aminoisobutyric Acids / Q000032:analysis* / Q000302:isolation & purification / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007202:Indicators and Reagents; D013056:Spectrophotometry, Ultraviolet; D015091:beta-Alanine / Q000652:urine
chemical_list: D000621:Aminoisobutyric Acids; D007202:Indicators and Reagents; D015091:beta-Alanine; C033435:3-aminoisobutyric acid