Isolation, characterization, and mapping of a human acid beta-galactosidase cDNA.
A lambda gt11 human testicular cDNA library was screened with degenerate oligonucleotide probe mixtures based on amino acid sequence data generated from cyanogen bromide fragments and tryptic fragments of purified human beta-galactosidase. Six positive clones were identified after screening 2 x 10(6) plaques. The sequences of these six clones were determined and found to be derived from two different cDNAs. The sequence of the longest of these cDNAs is nearly identical to that recently determined by Oshima et al. (1988). It codes for a 76-kD protein and all 11 peptides that were generated from the purified enzyme. The second clone is shorter by 393 bp in the central portion of the coding region. Analysis by Northern blotting revealed the presence of a single mRNA species of 2.45 kb in lymphoblasts and testicular tissue. It is deduced from the amino acid sequence data that proteolytic processing of the precursor form of beta-galactosidase must occur by cleavage in the carboxy-terminal portion of the polypeptide perhaps around amino acid 530 at a uniquely hydrophilic sequence. Using a probe generated from the 3' region of the cDNA, we have mapped the locus coding for human beta-galactosidase to chromosome 3p21-3pter.
Yamamoto
Y
Y
Department of Neurosciences, University of California, San Diego, School of Medicine, La Jolla, CA 92093.
Hake
C A
CA
Martin
B M
BM
Kretz
K A
KA
Ahern-Rindell
A J
AJ
Naylor
S L
SL
Mudd
M
M
O'Brien
J S
JS
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D005696:Galactosidases / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics*
D012333:RNA, Messenger; D004247:DNA; D005696:Galactosidases; C021502:acid beta-galactosidase; D001616:beta-Galactosidase