title: The use of two in-situ simultaneously-perfused placentas to investigate the effect of carrier proteins on linoleic and palmitic acid transfer in the guinea pig.
abstract: By perfusing two placentas concurrently in five animals we have found that linoleic acid is transferred across the guinea-pig placenta more readily than palmitic acid. In the in-situ perfusion this transfer is modulated by the type of albumin used in the perfusate. Removing the associated lipid from the perfusate albumin reduced the amount of non-esterified fatty acid (NEFA) transfer rather than enhanced it. Many researchers use fat free albumin as a protein carrier in in vivo and in-vitro systems, our results indicate that this may not be the most appropriate substance to use.
authors.affiliation: Division of Medicine, U.M.D.S., St. Thomas' Hospital, London, UK.
authors.forename: C R
authors.initials: CR
authors.lastname: Thomas
authors.forename: C
authors.initials: C
authors.lastname: Lowy
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000494:pharmacology*; D005260:Female; D006168:Guinea Pigs; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000493:pharmacokinetics*; D008431:Maternal-Fetal Exchange; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000493:pharmacokinetics* / Q000494:pharmacology; D010477:Perfusion; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D012709:Serum Albumin / Q000494:pharmacology
chemical_list: D002352:Carrier Proteins; D008041:Linoleic Acids; D010169:Palmitic Acids; D012709:Serum Albumin; D019308:Palmitic Acid; D019787:Linoleic Acid