Conditions for the extraction of protease-free LMG proteins from calf thymus.
Calf thymus LMG proteins are known to be susceptible to a wide variety of chromatin-associated proteases. We have investigated the use of different inhibitors to protect LMG chromosomal proteins from proteolytic degradation. In this work we have developed a preparative method for the extraction of LMG proteins from purified chromatin which provides an LMG protein extract devoid of proteolytic activity.
Durand
J P
JP
Laboratoire de Biochimie et Radiobiochimie, Faculté des Sciences, Université de Nantes, France.
Goudard
F
F
Pieri
J
J
Walker
J M
JM
D000818:Animals; D002417:Cattle; D002843:Chromatin / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification*; D010450:Endopeptidases / Q000302:isolation & purification; D011480:Protease Inhibitors; D013950:Thymus Gland / Q000737:chemistry*
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D011480:Protease Inhibitors; D010450:Endopeptidases