Preparation and duplex-to-single strand transition of the fully complementary duplex of d(G4).d(C4) in aqueous solution.
The d(G4) and d(C4) molecules in the single stranded state were synthesized by the phosphotriester method and purified. The full duplex of tetramer d(G4).d(C4) was prepared by expending about a month. The duplex-to-single strand transition was observed by UV-spectroscopy. A standard hypochromic effect was observed, which is different from some experimental results reported previously.
Shinoda
T
T
National Chemical Laboratory for Industry, Tsukuba Research Center, Ibaraki, Japan.
Wada
T
T
Ohuchi
S
S
Hata
T
T
D004277:DNA, Single-Stranded / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D011066:Poly C / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D011068:Poly G / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D011089:Polydeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D012996:Solutions; D014867:Water
D004277:DNA, Single-Stranded; D011089:Polydeoxyribonucleotides; D012996:Solutions; D014867:Water; D011068:Poly G; C023826:poly(dC); C026628:poly(dG); C019530:poly(dC-dG); D011066:Poly C