title: Nitrosoguanidine-induced adaptive repair in Pseudomonas aeruginosa.
abstract: Error-proof adaptive repair has been demonstrated in Pseudomonas aeruginosa. Cells of actively replicating wild-type Ps. aeruginosa (ATCC27853) and its auxotrophic derivative PAO 286 were subjected to stepwise adaptation (up to 1 microgram ml-1) by nitrosoguanidine (MNNG). Such cells resisted lethal and mutagenic effects of MNNG-challenge (lethal) doses more efficiently than those of nonadapted cultures. Similarly, reactivation of alkylated Pseudomonas phages was enhanced in adapted cells only. Induction of adaptive repair enzymes was sensitive to chloramphenicol (protein synthesis-inhibiting antibiotic) during adaptation treatment only. Protein extract from adapted cells showed increased levels in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE).
authors.affiliation: Department of Microbiology, University of Karachi, Pakistan.
authors.forename: S A
authors.initials: SA
authors.lastname: Rasool
authors.forename: A
authors.initials: A
authors.lastname: Mirza
authors.forename: M A
authors.initials: MA
authors.lastname: Khan
mesh_terms: D000222:Adaptation, Physiological; D000478:Alkylation; D002701:Chloramphenicol / Q000494:pharmacology; D004260:DNA Repair; D004269:DNA, Bacterial / Q000378:metabolism; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial; D008769:Methylnitronitrosoguanidine; D002701:Chloramphenicol