title: Multidimensional liquid chromatography of opioid peptides: fluorogenic labelling, retention prediction and separation optimization.
abstract: The ultra-trace analysis of opioid peptides in biological samples can be achieved by multidimensional liquid chromatography with pre-column fluorogenic derivatization with naphthalene-2,3-dicarboxaldehyde in the presence of cyanide ion. However, in order to take full advantage of the high sensitivity possible with detectors based on laser-induced fluorescence or chemiluminescence, each component of the analytical method must be carefully optimized. In this study, strategies are presented for the prediction of retention time and the optimization of separations of derivatized opioid peptides in multidimensional LC systems.
authors.affiliation: Fort Hays State University, Hays, KS 67601-4099.
authors.forename: L M
authors.initials: LM
authors.lastname: Nicholson
authors.forename: H B
authors.initials: HB
authors.lastname: Patel
authors.forename: F
authors.initials: F
authors.lastname: Kristjansson
authors.forename: S C
authors.initials: SC
authors.lastname: Crowley
authors.forename: K
authors.initials: K
authors.lastname: Dave
authors.forename: J F
authors.initials: JF
authors.lastname: Stobaugh
authors.forename: C M
authors.initials: CM
authors.lastname: Riley
mesh_terms: D000097:Acetonitriles; D000595:Amino Acid Sequence; D002853:Chromatography, Liquid; D003486:Cyanides; D004723:Endorphins / Q000032:analysis*; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives / Q000032:analysis; D007202:Indicators and Reagents; D007700:Kinetics; D007834:Lasers; D008163:Luminescent Measurements; D008969:Molecular Sequence Data; D009281:Naphthalenes; D013050:Spectrometry, Fluorescence; D013654:Taurine / Q000032:analysis
chemical_list: D000097:Acetonitriles; D003486:Cyanides; D004723:Endorphins; D007202:Indicators and Reagents; D009281:Naphthalenes; D013654:Taurine; D004744:Enkephalin, Methionine; C027717:2,3-naphthalenedicarboxaldehyde; C028718:enkephalin-Met, Lys(6)-