title: Laboratory evidence of active HIV-1 infection in Canadians with hemophilia associated with administration of heat-treated factor VIII.
abstract: Heat-treated factor VIII has been implicated in the transmission of HIV to hemophiliacs. Previously, evidence has been limited to documenting cases of seroconversion following administration of heat-treated factor VIII. Here, we present evidence of active HIV infection, i.e., infected and not merely sensitized following factor VIII injections. Six Canadians with hemophilia had seroconverted during a longitudinal study of their HIV immune status. Two of the three patients tested by this method demonstrated HIV gag-specific sequences upon amplification by polymerase chain reaction. In addition, HIV-1 virus was isolated from peripheral blood lymphocytes of one of these two persons as shown by reverse transcriptase activity of culture supernatants as well as neutralizable p24 antigen. This, we believe, is the first evidence of active HIV infection following administration of 60 degrees C, 30 h heat-treated factor VIII.
authors.affiliation: Federal Centre for AIDS, Health and Welfare Canada, Ottawa.
authors.forename: P
authors.initials: P
authors.lastname: Neumann
authors.forename: M
authors.initials: M
authors.lastname: O'Shaughnessy
authors.forename: R
authors.initials: R
authors.lastname: Remis
authors.forename: C
authors.initials: C
authors.lastname: Tsoukas
authors.forename: D
authors.initials: D
authors.lastname: Lepine
authors.forename: M
authors.initials: M
authors.lastname: Davis
mesh_terms: D002170:Canada; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005169:Factor VIII / Q000009:adverse effects*; D005784:Gene Amplification; D015658:HIV Infections / Q000150:complications / Q000209:etiology*; D006679:HIV Seropositivity; D015497:HIV-1 / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006467:Hemophilia A / Q000150:complications / Q000628:therapy*; D006358:Hot Temperature; D006801:Humans; D015151:Immunoblotting; D008137:Longitudinal Studies; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral; D005169:Factor VIII