Participation by opioids in the immunostimulatory activity of myelopeptides.
Bone marrow myelopeptides (MP), besides having immunostimulatory activity, had a pronounced dose-dependent effect on the development of pain sensitivity in mice. Nanogram amounts of MP evoked a hyperalgesic response and increased antibody formation to sheep red blood cells three to nine times. Milligram amounts of MP had a hypoalgesic effect and did not affect antibody response. Opioid peptides derived from the bone marrow MP are involved in the expression of the antibody response, and a mixture of synthetic opioids, corresponding in composition to that found in natural MP, stimulated antibody production. The antibody-stimulating effect of MP was abolished by naloxone. Of the opioid peptides, only beta-endorphin showed antibody-stimulating activity. On reversed-phase chromatography the antibody-stimulating peptides and beta-endorphin were eluted in different fractions, indicating that the immunostimulatory and opioid activities are produced by different peptide molecules.
Zakharova
L A
LA
Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Moscow.
Belevskaya
R G
RG
Yanovskii
O G
OG
D000276:Adjuvants, Immunologic; D000700:Analgesics / Q000276:immunology*; D000818:Animals; D000917:Antibody Formation; D001853:Bone Marrow / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004723:Endorphins / Q000138:chemical synthesis / Q000276:immunology*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009842:Oligopeptides; D010147:Pain Measurement; D010455:Peptides / Q000276:immunology* / Q000302:isolation & purification
D000276:Adjuvants, Immunologic; D000700:Analgesics; D004723:Endorphins; D009842:Oligopeptides; D010455:Peptides; C048617:myelopeptides