[Isolation of tubular proximal cells of the human kidney by affinity chromatography using Lotus tetragonolobus lectin].
An affinity chromatography technique, using a lectin issued from Lotus tetragonolobus as ligand, and coupled to Sepharose 6MB support, has been chosen to enrich a cellular population in tubular proximal cells. Cell desorption was obtained with a competitive sugar, L Fucose. Intensive enzymatic brush border activity, shown in selected cells compared with initial cellular mixture, evidenced an enrichment in proximal tubular cells. These results prove that lectin use as ligand in affinity chromatography permits the separation of renal cell populations and presents the advantage that the bound cells can be recovered by a gentle competitive elution for further cultures.
Merlet
D
D
Laboratoire de Biologie cellulaire, Faculté de Pharmacie, Bordeaux.
Merlet
J P
JP
Cambar
J
J
D002469:Cell Separation / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods*; D005643:Fucose; D006801:Humans; D007687:Kidney Tubules, Proximal / Q000166:cytology*; D037102:Lectins
D037102:Lectins; D005643:Fucose
Isolement de cellules tubulaires proximales de reins humains par chromatographie d'affinité grâce à la lectine de Lotus tetragonolobus.