title: Comparison of promoter activities in Escherichia coli and Pseudomonas aeruginosa: use of a new broad-host-range promoter-probe plasmid.
abstract: The broad-host-range plasmid, pRW2, is a derivative of pRK 2501 carrying the Escherichia coli lac operon without a promoter, downstream of a polylinker sequence. We have cloned a number of DNA fragments carrying promoters into this plasmid and measured promoter activity in both E. coli and Pseudomonas aeruginosa. Promoters carrying consensus -10 and -35 sequences were active in both backgrounds and the dependence of activity on the nucleotide sequence of the 35 region was the same in both cases. We also measured the activity of two promoters at which transcription in E. coli was totally dependent on the E. coli activators CRP and FNR: both promoters were found to be active in P. aeruginosa.
authors.affiliation: School of Biochemistry, University of Birmingham, U.K.
authors.forename: J
authors.initials: J
authors.lastname: Lodge
authors.forename: R
authors.initials: R
authors.lastname: Williams
authors.forename: A
authors.initials: A
authors.lastname: Bell
authors.forename: B
authors.initials: B
authors.lastname: Chan
authors.forename: S
authors.initials: S
authors.lastname: Busby
mesh_terms: D001483:Base Sequence; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial