Effect of ultraviolet-B radiation on protein synthesis in cultured lens epithelial cells.
Exposure of cultured rabbit lens epithelial cells to repetitive doses of UV-B radiation delays their growth and alters the synthesis of specific proteins. Irradiated cells on the shoulder of the survival curve exhibited a dose-dependent decrease in growth when subcultured in serum-supplemented medium. UV-B irradiation did not affect the subsequent attachment efficiency of the cells. Control and UV-B irradiated cells were incubated with [35S]methionine and the pattern of protein synthesis in the cells was analyzed by SDS-PAGE and autoradiography. Analysis of the labeled proteins from cells exposed to UV-B radiation showed the induction of a 32 kD polypeptide and the loss of a 26 kD polypeptide compared with controls. Analysis of the proteins released by the UV-B irradiated cells into the culture medium revealed the 50% loss of a 37 kD radiolabeled protein compared with controls. The alteration of protein synthesis in lens epithelial cells by UV-B radiation may contribute to cataract formation.
Andley
U P
UP
Howe Laboratory of Ophthalmology, Harvard Medical School, Boston, MA.
Walsh
A
A
Kochevar
I E
IE
Reddan
J R
JR
D000818:Animals; D001345:Autoradiography; D002448:Cell Adhesion / Q000528:radiation effects; D002455:Cell Division / Q000528:radiation effects; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D003459:Crystallins / Q000096:biosynthesis* / Q000528:radiation effects; D004591:Electrophoresis, Polyacrylamide Gel; D004848:Epithelium / Q000528:radiation effects; D007908:Lens, Crystalline / Q000378:metabolism / Q000528:radiation effects*; D008715:Methionine / Q000494:pharmacology; D011817:Rabbits; D014466:Ultraviolet Rays
D003459:Crystallins; D008715:Methionine