Immunoelectron microscopic localization of thyrotropin-releasing hormone (TRH) precursor in the rat raphe nuclei.
Using antisera to two pro-thyrotropin-releasing hormone (pro-TRH)-derived cryptic peptides, we have studies by immunocytochemistry the ultrastructural localization of pro-TRH in the rat raphe nuclei. The same results were obtained with both antisera. Immunostaining was found in cell bodies, dendrites and endings. In cell bodies, the reaction product was restricted to Golgi saccules and dense core vesicles which were very few in number. In dendrites, the staining was rather diffuse without any association with specific organelles. These results suggest that the Golgi apparatus might be involved in pro-TRH processing.
Liao
N
N
MRC Group in Molecular Endocrinology, Laval University Medical Center, Quebec, Canada.
Bulant
M
M
Nicolas
P
P
Vaudry
H
H
Pelletier
G
G
D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D011498:Protein Precursors / Q000032:analysis* / Q000235:genetics; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D011903:Raphe Nuclei / Q000032:analysis* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D013973:Thyrotropin-Releasing Hormone / Q000032:analysis* / Q000235:genetics
D010446:Peptide Fragments; D011498:Protein Precursors; D013973:Thyrotropin-Releasing Hormone; C046418:pro-thyrotropin releasing hormone; D011761:Pyrrolidonecarboxylic Acid