Structural modifications induced by the mtDBP-C protein in the replication origin of Xenopus laevis mitochondrial DNA.
The structure of the non-coding region of Xenopus laevis mitochondrial DNA has been studied by electron microscopy analysis of DNA molecules end-labelled with streptavidin-ferritin. We have shown that the effect of a protein modifying the shape of the DNA double-helix can be studied and precisely located by this method. It was found that the non-coding region contains curved segments and that the mitochondrial protein mtDBP-C preferentially enhances the curvature of the promoters-replication origin region.
Mignotte
B
B
Laboratoire de Biologie Générale, Université de Paris Sud, Orsay, France.
Theveny
B
B
Revet
B
B
D000818:Animals; D001483:Base Sequence; D004261:DNA Replication; D004272:DNA, Mitochondrial / Q000648:ultrastructure; D004268:DNA-Binding Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D014982:Xenopus laevis / Q000235:genetics*
D004272:DNA, Mitochondrial; D004268:DNA-Binding Proteins