An improved colorimetric assay for fructosamine.
With the commercial availability of the improved colorimetric assay for fructosamine, we established the fructosamine values for non-diabetic healthy persons. This improved assay incorporates detergent and uricase in a more concentrated nitroblue-tetrazolium reagent base compared to the earlier reagent (Johnson & Baker assay), including a new serum calibrator. An evaluation of the assay showed that fructosamine values obtained with serum, EDTA-plasma and heparin-plasma were comparable to each other. There was no apparent effect on fructosamine by lipids. Within-run precision variations for each specimen type (fructosamine 248-592 mumol/l), determined with a mechanised analyser were less than 3% (0.9-3%). Between-run precision for serum and EDTA-plasma ranged from 2.5% to 5.6%. This fructosamine assay also showed a positive correlation with the Johnson and Baker assay. Based on 48 healthy adults (20 males, 28 females) of mean age 32.9 +/- 9.2 years, the fructosamines ranged from 195 to 304 mumol/l, with a mean 250 +/- 24.3 mumol/l. The 2.5 and 97.5 percentiles were 196.4 mumol/l and 301.8 mumol/l respectively.
Ng
W Y
WY
Department of Medicine, National University of Singapore.
Lui
K F
KF
Thai
A C
AC
D000328:Adult; D002784:Cholesterol / Q000097:blood; D003124:Colorimetry / Q000379:methods*; D003920:Diabetes Mellitus / Q000097:blood; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D019270:Fructosamine; D006493:Heparin / Q000494:pharmacology; D006595:Hexosamines / Q000097:blood*; D006801:Humans; D008297:Male; D014280:Triglycerides / Q000097:blood
D006595:Hexosamines; D014280:Triglycerides; D019270:Fructosamine; D006493:Heparin; D002784:Cholesterol; D004492:Edetic Acid