Isolation and characterization of mutations in the gene encoding an endogenous Bacillus subtilis beta-galactosidase and its regulator.
We have isolated mutations that appear to inactivate the gene (lacA) encoding an endogenous beta-galactosidase activity in Bacillus subtilis and in a closely linked negative regulatory element (lacR). Both genes map to the hisA-thrA region. The lacA mutations may help to avoid some of the problems arising from the use of the Escherichia coli lacZ gene as a reporter gene in B. subtilis.
Errington
J
J
Department of Biochemistry, University of Oxford, United Kingdom.
Vogt
C H
CH
D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D002874:Chromosome Mapping; D005696:Galactosidases / Q000235:genetics*; D005798:Genes, Bacterial; D005809:Genes, Regulator; D009154:Mutation; D001616:beta-Galactosidase / Q000235:genetics*
D005696:Galactosidases; D001616:beta-Galactosidase