title: An in vitro model for cyclosporin A-induced interference of intrathymic clonal elimination.
abstract: The effects of cyclosporin A (CsA) on influencing the intrathymic clonal deletion were investigated by using our established thymic stromal cell clone with capacities to express Ia antigens and to produce a unique T cell growth factor. The following were revealed: (a) T cell clone with a given specificity was killed on the Ia+ stromal cell monolayer in the presence of the relevant antigens, a process depending on T cell receptor (TCR) stimulation; and (b) CsA allowed the T cell clone to continuously proliferate even during TCR stimulation by virtue of the stromal cell-derived T cell growth factor. This paper describes an in vitro model of a mechanism by which CsA is responsible for the generation of normally "forbidden" T cell clones.
authors.affiliation: Biomedical Research Center, Osaka University Medical School, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kosaka
authors.forename: H
authors.initials: H
authors.lastname: Matsubara
authors.forename: S
authors.initials: S
authors.lastname: Sogoh
authors.forename: M
authors.initials: M
authors.lastname: Ogata
authors.forename: T
authors.initials: T
authors.lastname: Hamaoka
authors.forename: H
authors.initials: H
authors.lastname: Fujiwara
mesh_terms: D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D003524:Cyclosporins / Q000494:pharmacology*; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000494:pharmacology; D007376:Interleukin-2 / Q000378:metabolism; D051379:Mice; D008817:Mice, Mutant Strains; D008954:Models, Biological; D011948:Receptors, Antigen, T-Cell / Q000096:biosynthesis / Q000187:drug effects; D011994:Recombinant Proteins; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects*
chemical_list: D003524:Cyclosporins; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D011948:Receptors, Antigen, T-Cell; D011994:Recombinant Proteins; D007371:Interferon-gamma