Cloning and characterization of a cDNA encoding the wheat (Triticum durum Desf.) CM16 protein.
A Triticum durum cDNA library prepared from developing endosperm (22 days after flowering (DAF] was screened using synthetic oligonucleotide probes covering part of the CM3 and CM16 N-terminal protein sequences. A full-length cDNA clone (pTd78) encoding the CM16 protein (chloroform/methanol-soluble protein) was isolated and characterized. To our knowledge this is the first characterization of a clone coding for a wheat CM protein. The CM16 protein is synthesized as a preprotein with a signal peptide of 24 residues, the molecular weight of the mature protein being 13,438 Da. As other members of the cereal trypsin/alpha-amylase inhibitor family, the CM16 protein contains 10 cysteine residues, their position being well conserved. In developing endosperm the highest level of CM16 mRNA was detected at mid-maturation.
Gautier
M F
MF
Laboratoire de Technologie des Céréales, INRA, Montpellier, France.
Alary
R
R
Joudrier
P
P
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D063245:Plant Development; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D014908:Triticum / Q000235:genetics / Q000254:growth & development
D010940:Plant Proteins; D004247:DNA