Studies on C17 brown mice subjected to photochemotherapeutic doses of long wave length ultraviolet (UVA) radiation.
In this study the risk of photochemotherapeutic dose levels of long wave ultraviolet radiation (UVA) was assessed by employing a laboratory animal system, C17 brown mice. The experimental group was subjected to three UVA dose levels, 1000, 2000 and 3500 J/cm2. The dose regimen 50 J/cm2 per day for five days a week was completed in 4, 8 and 14 weeks respectively. The UVA exposed animals were examined until 52 weeks post UVA exposure periods for morphological lesions. Estimations of DNA, protein levels and dermal, epidermal thickness were made. There were no lesions observed with the highest UVA dose employed. Alterations in the DNA and protein levels in the skin of animals in the exposed groups were observed in the post UVA periods. A notable increase in the DNA level was observed 47 weeks post UVA period. The significance of alterations in DNA and protein levels needs to be studied further for evaluation of long term risk following UVA exposure. The data presented however led to a conclusion that the photochemotherapeutic doses of UVA do not pose any risk of cancer to pigmented mouse strains.
Trivedi
P N
PN
Radiobiology Unit, Tata Memorial Centre, Parel, Bombay, India.
Bamane
V S
VS
Gadgil
R B
RB
Ranade
S S
SS
D000818:Animals; D004247:DNA / Q000032:analysis / Q000187:drug effects / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005260:Female; D008730:Methoxsalen / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D010778:Photochemotherapy; D011506:Proteins / Q000032:analysis / Q000528:radiation effects; D012016:Reference Values; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects*; D013997:Time Factors; D014466:Ultraviolet Rays
D011506:Proteins; D004247:DNA; D008730:Methoxsalen