title: Patterns and extent of isotype-specificity in the murine H chain switch DNA rearrangement.
abstract: We have analyzed the configuration of the H chain locus of 41 hybridomas by Southern blot analysis. Each H chain switch region was determined to be germ line, rearranged, or deleted. Including 13 previously analyzed hybridomas, 60% of those with rearrangements on both alleles showed a correlation of the two alleles, i.e., both the expressed and the nonexpressed alleles have rearranged to the same H chain constant region gene segment. When the two H chain alleles did not rearrange to the same gene, they often rearranged to neighboring H chain genes. These results support a role for isotype-specific factors in H chain switch recombination. The action of these isotype-specific factors may be propagated to some extent along the chromosome, which would lead to rearrangements to neighboring genes.
authors.affiliation: Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109-0620.
authors.forename: C
authors.initials: C
authors.lastname: Schultz
authors.forename: J
authors.initials: J
authors.lastname: Petrini
authors.forename: J
authors.initials: J
authors.lastname: Collins
authors.forename: J L
authors.initials: JL
authors.lastname: Claflin
authors.forename: K A
authors.initials: KA
authors.lastname: Denis
authors.forename: P
authors.initials: P
authors.lastname: Gearhart
authors.forename: C
authors.initials: C
authors.lastname: Gritzmacher
authors.forename: T
authors.initials: T
authors.lastname: Manser
authors.forename: M
authors.initials: M
authors.lastname: Shulman
authors.forename: W
authors.initials: W
authors.lastname: Dunnick
mesh_terms: D000483:Alleles; D000818:Animals; D015139:Blotting, Southern; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D005803:Genes, Immunoglobulin; D006825:Hybridomas; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007132:Immunoglobulin Isotypes / Q000235:genetics; D051379:Mice
chemical_list: D007143:Immunoglobulin Heavy Chains; D007132:Immunoglobulin Isotypes