Enhancement of sickle erythrocyte adherence to endothelium by autologous platelets.
The increased adhesiveness of sickle erythrocytes (SS RBC) to endothelial cells has been confirmed in a static system utilizing fresh umbilical vein endothelium. Adherence of SS RBC to the endothelium was as great in the presence of calcium-containing buffer as when incubated in plasma. SS RBC suspended in autologous platelet-rich plasma adhered to a greater extent than when suspended in autologous platelet poor plasma. Prostacyclin, thromboxane B2, and an inhibitor of collagen- and epinephrine-induced platelet aggregation (B13.177) did not affect SS RBC adherence to endothelium. Aspirin in a concentration of 5 micrograms/ml slightly decreased SS RBC adherence to endothelium in the presence of platelets. Platelets may play a significant role in the increased adhesiveness of SS RBC to endothelium. To the extent that increased SS RBC adhesiveness contributes to the genesis of painful crises and to the extent platelets augment this adhesiveness, agents affecting platelet function may prove useful in preventing painful crises.
Antonucci
R
R
Department of Medicine, University of North Carolina School of Medicine, Chapel Hill 27514.
Walker
R
R
Herion
J
J
Orringer
E
E
D000755:Anemia, Sickle Cell / Q000097:blood*; D001241:Aspirin / Q000494:pharmacology; D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D002448:Cell Adhesion / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D011464:Epoprostenol / Q000494:pharmacology; D004903:Erythrocyte Aggregation / Q000187:drug effects; D004912:Erythrocytes / Q000032:analysis; D004913:Erythrocytes, Abnormal / Q000378:metabolism / Q000473:pathology*; D006451:Hemoglobin, Sickle / Q000032:analysis; D006801:Humans; D013929:Thromboxane B2 / Q000494:pharmacology
D006451:Hemoglobin, Sickle; D013929:Thromboxane B2; D011464:Epoprostenol; D001241:Aspirin