Capsaicin-sensitive vagal afferents contribute to gastric acid and vascular responses to intracisternal TRH analog.
Central injection of TRH or its stable analog, RX77368, produces a vagal cholinergic stimulation of gastric acid secretion, mucosal blood flow and motor function. In the present study, we have investigated the contribution of capsaicin-sensitive vagal afferent fibers to the gastric responses to intracisternal injection of RX77368. Gastric acid secretion, measured in acute gastric fistula rats anesthetized with urethane, in response to intracisternal injection of RX77368 (3-30 ng) was reduced by 21-65% by perineural pretreatment of the vagus nerves with capsaicin 10-20 days before experiments. The increase in gastric mucosal blood flow measured by hydrogen gas clearance induced by intracisternal injection of RX77368 (30 ng) was also reduced by 65% in capsaicin-pretreated rats. In contrast, increases in gastric motor function measured manometrically or release of gastric luminal serotonin in response to intracisternal injection of RX77368 (3-30 ng) were unaltered by capsaicin pretreatment. The mechanism by which vagal afferent fibers contribute to the secretory and blood flow responses to the stable TRH analog is unclear at present, but it is possible that the decrease in gastric mucosal blood flow by lesion of capsaicin-sensitive vagal afferents limits the secretory response.
Raybould
H E
HE
Center for Ulcer Research and Education, VA Wadsworth Medical Center, Los Angeles, CA 90073.
Holzer
P
P
Reddy
S N
SN
Yang
H
H
Taché
Y
Y
D000344:Afferent Pathways / Q000187:drug effects; D000818:Animals; D001808:Blood Vessels / Q000187:drug effects; D002211:Capsaicin / Q000494:pharmacology*; D002946:Cisterna Magna; D005744:Gastric Acid / Q000378:metabolism; D005753:Gastric Mucosa / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism; D005769:Gastrointestinal Motility / Q000187:drug effects; D007267:Injections; D008297:Male; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D051381:Rats; D011919:Rats, Inbred Strains; D012039:Regional Blood Flow; D012701:Serotonin / Q000378:metabolism; D013973:Thyrotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000494:pharmacology; D014630:Vagus Nerve / Q000187:drug effects*
D012701:Serotonin; D013973:Thyrotropin-Releasing Hormone; C027979:L-pyroglutamyl-L-histidyl-3,3-dimethylprolinamide; D002211:Capsaicin; D011761:Pyrrolidonecarboxylic Acid