The effect of transforming growth factor-beta 1 on glycosaminoglycan production by human marrow cultures.
We have assayed the effect of transforming growth factor-beta 1 (TGF-beta 1), a potent modulator of hematopoiesis, on glycosaminoglycan production in human marrow cultures. Glycosaminoglycans are a component of the extracellular matrix known to affect cell growth and differentiation. TGF-beta 1 and [35S]sulfate were added simultaneously to hematopoietically active human marrow cultures, and radiolabeled glycosaminoglycan production was determined by cetylpyridinium chloride precipitation. TGF-beta 1 at 15 ng/ml for 72 h increased [35S]sulfate incorporation into media glycosaminoglycans to 190% of control levels but did not affect the [35S]sulfate incorporation into cell-associated glycosaminoglycans. Approximately 90% of the glycosaminoglycans in the media fraction and 85% of the glycosaminoglycans in the cell-associated fraction were susceptible to degradation by chondroitin ABCase in both treated and control cultures. Pulse-chase experiments suggested that the increase in glycosaminoglycan [35S]sulfate incorporation was not due to decreased glycosaminoglycan degradation. This concentration of TGF-beta 1 did not alter nonadherent granulocyte-macrophage colony-forming unit (CFU-GM) number per flask but significantly decreased the more primitive adherent CFU-GM number per flask (by 50%-70%). These data suggest that the ability of TGF-beta 1 to modulate hematopoiesis may be due, in part, to its effects on glycosaminoglycan production.
Uhlman
D L
DL
Department of Medicine, University of Minnesota Medical School, Minneapolis.
Mooradian
D L
DL
Furcht
L T
LT
Luikart
S D
SD
D001853:Bone Marrow / Q000378:metabolism*; D002455:Cell Division; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000096:biosynthesis; D003114:Colony-Forming Units Assay; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006098:Granulocytes / Q000166:cytology; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006497:Heparan Sulfate / Q000096:biosynthesis; D006801:Humans; D008264:Macrophages / Q000166:cytology; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D002809:Chondroitin Sulfates; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D016212:Transforming Growth Factor beta