title: [Cloning and expression in Bacillus subtilis of the gene for neutral protease of Bacillus brevis].
abstract: Plasmids pCB20 and pCB22 were used for cloning and expression of the Bac brevis 7882 neutral protease gene in Bac. subtilis cells. The protease-containing fragments of 13 and 14 kb were cloned in pCB20 plasmid based on replication region of Streptococci plasmid pSM19035. Expression of the gene was shown to take place in Bac. subtilis. Application of vegetative promoters of the previously identified expression unit EU19035 greatly increases the expression of the protease in Bac. subtilis. Bac. subtilis cells, expressing the gene of Bac. brevis neutral protease, do not sporulate, are considerably larger than the cells which do not contain the gene and form multicellular structures.
authors.forename: A S
authors.initials: AS
authors.lastname: Avakov
authors.forename: A P
authors.initials: AP
authors.lastname: Bolotin
authors.forename: L G
authors.initials: LG
authors.lastname: Kolibaba
authors.forename: A V
authors.initials: AV
authors.lastname: Sorokin
authors.forename: T M
authors.initials: TM
authors.lastname: Shemiakina
authors.forename: M
authors.initials: M
authors.lastname: Paberit
authors.forename: Kh
authors.initials: Kh
authors.lastname: Raĭk
authors.forename: A
authors.initials: A
authors.lastname: Aaviksaar
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D001412:Bacillus subtilis / Q000235:genetics*; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005798:Genes, Bacterial; D005822:Genetic Vectors; D008666:Metalloendopeptidases / Q000235:genetics*; D010957:Plasmids; D015183:Restriction Mapping
chemical_list: D008666:Metalloendopeptidases; C044194:microbial metalloproteinases
vernacular_title: Klonirovanie i ékspressiia v Bacillus subtilis gena neĭtral'noĭ proteazy Bacillus brevis.