Radioimmunoassay of corticotropin-releasing hormone.
A specific double antibody radioimmunoassay (RIA) for human-Corticotropin-Releasing Hormone (hCRF) using an antibody to synthetic hCRF was established. This antibody allowed a usable range of 10 pg to 5 ng of CRF per ml in the assay. Comparing the efficiency of various plasma extraction procedures, the extraction with ice-cold methanol was found to be the most simple and rapid method with an extraction efficiency of more than 80%. The reliability of the radioimmunoassay was shown under physiological and pathophysiological conditions.
Rückert
Y
Y
Department of Internal Medicine, Humboldt University Medical School (Charité), Academy of Sciences, GDR, Berlin.
Rohde
W
W
Furkert
J
J
D000293:Adolescent; D000328:Adult; D000818:Animals; D000917:Antibody Formation; D001667:Binding, Competitive; D003346:Corticotropin-Releasing Hormone / Q000032:analysis* / Q000097:blood; D003480:Cushing Syndrome / Q000097:blood; D006801:Humans; D007457:Iodine Radioisotopes / Q000032:analysis; D000432:Methanol; D008875:Middle Aged; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity
D007457:Iodine Radioisotopes; D003346:Corticotropin-Releasing Hormone; D000432:Methanol