title: Amelogenin gene similarity in vertebrates: DNA sequences encoding amelogenin seem to be conserved during evolution.
abstract: Mouse amelogenin cDNA was used in hybridization assays with genomic DNA, cut with the restriction enzyme Eco RI, from the edentulous chicken (Gallus domesticus), the monophyodont mouse (as control), diphyodont man, and the polyphyodont fishes Atlantic salmon (Salmo salar) and seawolf (Anarrhichas lupus). The hybridization assay was performed under stringent conditions with non-radioactive probes. Hybridization was obtained with mouse (6.4-kb band), man (9-kb and 13-kb bands), and seawolf (18-kb band) genomic DNA. This demonstrates DNA sequence similarities between these species, and supports the theory that DNA sequences encoding enamel proteins appear to be highly conserved during the evolution of vertebrates. Lack of hybridization in salmon and chicken may be due to sequence divergences or structural differences in an amelogenin gene analog, or it may be that no amelogenin gene is present in these animals.
authors.affiliation: Department of Anatomy, Dental Faculty, University of Oslo, Norway.
authors.forename: S P
authors.initials: SP
authors.lastname: Lyngstadaas
authors.forename: S
authors.initials: S
authors.lastname: Risnes
authors.forename: H
authors.initials: H
authors.lastname: Nordbø
authors.forename: A G
authors.initials: AG
authors.lastname: Flønes
mesh_terms: D053523:Amelogenin; D000818:Animals; D005075:Biological Evolution; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D003746:Dental Enamel Proteins / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D012689:Sequence Homology, Nucleic Acid; D014714:Vertebrates / Q000235:genetics*
chemical_list: D053523:Amelogenin; C507200:Amelx protein, mouse; D015342:DNA Probes; D003746:Dental Enamel Proteins; D004247:DNA