Enhanced secretion of endothelin-1 by elevated glucose levels from cultured bovine aortic endothelial cells.
We have investigated the effect of glucose on the release of endothelin-1-like immunoreactivity (ET-1-LI) from cultured bovine aortic endothelial cells. Elevation of glucose concentrations in cultured media from 5.5 to 11.1 or 22.2 mM significantly stimulated ET-1-LI release from cultured endothelial cells. An aldose reductase inhibitor did not affect the high glucose-induced ET-1-LI release. These findings suggest the possibility that hyperglycemia in diabetic patients enhances ET-1-LI release at the local site of vascular endothelium, which might be involved in the developments of vascular complications and atherosclerosis.
Yamauchi
T
T
Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Ohnaka
K
K
Takayanagi
R
R
Umeda
F
F
Nawata
H
H
D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D016232:Endothelins; D004727:Endothelium / Q000187:drug effects / Q000378:metabolism; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D008353:Mannitol / Q000494:pharmacology; D010455:Peptides / Q000378:metabolism*; D010793:Phthalazines / Q000494:pharmacology
D016232:Endothelins; D010455:Peptides; D010793:Phthalazines; C042829:ponalrestat; D008353:Mannitol; D005947:Glucose