Blockade of the selective increase in serum follicle-stimulating hormone concentration in immature female rats and its effects on ovarian follicular development.
We investigated whether administration of monosodium L-glutamate (MSG) to neonatal female rats would block the selective increase in serum follicle-stimulating hormone (FSH) concentration in immature rats in an attempt to provide a model in which to study the importance of the selective FSH rise on ovarian follicular development. In two separate experiments, s.c. injections of MSG (4 mg/g BW) on Days 1, 3, 5, 7 and 9 after birth blocked the selective increase in serum FSH concentration observed on Days 7 and 15 without blocking basal FSH secretion. Serum luteinizing hormone (LH) levels were unaffected in the first experiment and changed little in the second. MSG-treated rats had smaller ovaries on Days 15 and 23. The ovaries of MSG-treated rats on Day 15 showed decreased follicular growth as evidenced by a decrease in the number and percentage of follicles with diameters greater than 50 microns, in the number of follicles with greater than 1 layer of granulosa cells, and in the number of follicles beyond the primary stage of follicular development. These differences between MSG-treated rats and controls all but disappeared by Day 23. The results demonstrate that neonatal administration of MSG blocks the selective increase in serum FSH concentration in immature female rats and suggest that this selective increase in serum FSH levels plays a role in the normal acceleration of ovarian follicular development but is not needed for the development of preovulatory follicles by the sixth week after birth.
Fagbohun
C F
CF
Department of Anatomy, Cell Biology and Neurosciences, University of South Carolina School of Medicine, Columbia 29208.
Dada
M O
MO
Metcalf
J P
JP
Ashiru
O A
OA
Blake
C A
CA
D000818:Animals; D000831:Animals, Newborn; D001835:Body Weight; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood*; D005971:Glutamates / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000097:blood; D009929:Organ Size; D006080:Ovarian Follicle / Q000187:drug effects / Q000254:growth & development*; D010053:Ovary / Q000033:anatomy & histology; D010903:Pituitary Gland, Anterior / Q000033:anatomy & histology / Q000378:metabolism; D011247:Pregnancy; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D012970:Sodium Glutamate / Q000494:pharmacology*
D005971:Glutamates; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D012970:Sodium Glutamate