Comparison of the outer membrane protein and lipopolysaccharide profiles of mucoid and nonmucoid Pseudomonas aeruginosa.
Laboratory-derived mucoid variants of Pseudomonas aeruginosa were selected by plating the standard PAO1 laboratory strain with bacteriophage. These mucoid variants formed two distinct groups of strains on the basis of phage typing. The first group had the same phage-typing pattern as the parent PAO1 strain, while the second group had a distinctly different phage-typing pattern. One strain from each group was assessed along with the parent PAO1 strain for its outer membrane protein (OMP) and lipopolysaccharide (LPS) profiles by sodium dodecyl sulfate-gel electrophoresis followed by appropriate staining. The mucoid derivatives were found to differ from the parent PAO1 nonmucoid strain in having lost a high-molecular-weight LPS species. Furthermore, the reversion of the mucoid strains to the nonmucoid phenotype was accompanied by a return of the missing high-molecular-weight LPS species. No observable difference between the mucoid derivatives and the parent nonmucoid strain was noted in the OMP profiles. The opposite was found in the case of four isolates of mucoid P. aeruginosa from patients with cystic fibrosis. Two OMP bands (of approximately 55 and 25 kilodaltons) were present in the mucoid isolates but missing in their sister nonmucoid strains. In the case of the cystic fibrosis isolates, no difference in the LPS profiles within mucoid-nonmucoid pairs was noted.
Kelly
N M
NM
Department of Microbiology, University of British Columbia, Vancouver, Canada.
MacDonald
M H
MH
Martin
N
N
Nicas
T
T
Hancock
R E
RE
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure; D003550:Cystic Fibrosis / Q000382:microbiology; D006801:Humans; D008070:Lipopolysaccharides / Q000032:analysis*; D008855:Microscopy, Electron, Scanning; D011550:Pseudomonas aeruginosa / Q000032:analysis* / Q000145:classification / Q000648:ultrastructure; D013045:Species Specificity
D001425:Bacterial Outer Membrane Proteins; D008070:Lipopolysaccharides