Oxygen-derived free radical reactions in experimental acute pancreatitis of the dog.
Acute edematous and necrotic pancreatitis have been induced in dogs with retrogradely intraductal injections of 5% hydrogen peroxide solution and sunflower-oil. The process of disease could be followed daily by a zipper sutured into the abdominal wound. In this manner the temporal changes of markers of oxygen-derived free radicals (concentrations of malondialdehyde and reduced glutathione of the excised pancreas tissue and abdominal exudate, as well as the superoxide dismutase content of the tissue) could be controlled. Light microscopic analysis was also done. In edematous pancreatitis reversible membrane lesions, in the necrotic form the irreversible damage of membranes and cells could be seen. The results obtained suggest the role of oxygen-derived free radicals in experimental acute pancreatitis.
Kelemen
D
D
Department of Experimental Surgery, University Medical School, Pécs, Hungary.
Török
B
B
D000208:Acute Disease; D000818:Animals; D004285:Dogs; D005260:Female; D005609:Free Radicals; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000008:administration & dosage; D008315:Malondialdehyde / Q000378:metabolism; D010195:Pancreatitis / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology*; D013482:Superoxide Dismutase / Q000378:metabolism
D005609:Free Radicals; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D005978:Glutathione