title: Praziquantel-induced secretion of proteolytic enzyme from Schistosoma mansoni cercariae.
abstract: The effect of praziquantel (PZQ) on secretion of proteolytic enzyme by Schistosoma mansoni cercariae was examined using an azocoll assay. The cercariae secreted proteolytic enzyme in various concentrations of PZQ (0.1, 1, and 10 micrograms/ml), but secretion of enzyme was highest at the lowest concentration. PZQ-induced secretion of proteolytic enzyme was partially inhibited by treatment with verapamil and ethylene glycol-bis(beta-aminoethyl ether)N,N,N',N'-tetra-acetic acid but not by calmodulin antagonist W-7 and protein kinase C inhibitor H-7.
authors.affiliation: Department of Parasitology, Nagasaki University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Matsumura
authors.forename: M
authors.initials: M
authors.lastname: Shimada
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: Y
authors.initials: Y
authors.lastname: Aoki
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004533:Egtazic Acid / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D007546:Isoquinolines / Q000494:pharmacology; D010447:Peptide Hydrolases / Q000096:biosynthesis*; D010879:Piperazines / Q000494:pharmacology; D011223:Praziquantel / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D012550:Schistosoma mansoni / Q000187:drug effects / Q000201:enzymology*; D013449:Sulfonamides / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
chemical_list: D007546:Isoquinolines; D010879:Piperazines; D013449:Sulfonamides; D004533:Egtazic Acid; D011223:Praziquantel; C017967:W 7; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D014700:Verapamil; D010447:Peptide Hydrolases