Comparison of osmium/sonication and EDTA/sonication microdissection techniques in exposing the adepithelial basal lamina surface of developing rat colon.
We have used two epithelial-stripping techniques in our studies of the basal lamina in the developing rat colon. The first involves prolonged osmication followed by sonication; the second uses chelation of calcium by EDTA followed by sonication. Both techniques remove the epithelium from the basal lamina; however, the EDTA/sonication technique appears to produce a cleaner adepithelial surface of the basal lamina. In addition, the fine structure of the basal lamina appears to be better preserved in specimens prepared by the EDTA/sonication technique. In contrast, the basal lamina of specimens prepared by the osmium/sonication technique had a shattered appearance that we believe is due to an increase in the fragility of the delicate fetal basal lamina.
McCarthy
K J
KJ
Department of Anatomy, Albany Medical College, New York 12208.
Kaye
G I
GI
D000818:Animals; D000831:Animals, Newborn; D001485:Basement Membrane / Q000254:growth & development / Q000648:ultrastructure*; D003106:Colon / Q000254:growth & development / Q000648:ultrastructure*; D004492:Edetic Acid; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D009992:Osmium; D051381:Rats; D013010:Sonication; D014465:Ultrasonics
D009992:Osmium; D004492:Edetic Acid