title: Light and electron microscope immunocytochemical localization of 5- and 12-lipoxygenases and cyclooxygenase enzymes in human granulosa cells from preovulatory follicles.
abstract: Cellular and subcellular distribution of 5- and 12-lipoxygenases and cyclooxygenase enzymes were investigated in human granulosa cells from preovulatory follicles using light and electron microscope immunocytochemistry. The results demonstrated that all three enzymes are present in granulosa cells but not in minor contaminating red blood cells. While the distribution of cyclooxygenase and 12-lipoxygenase was relatively uniform among the granulosa cells, 5-lipoxygenase was not uniformly distributed among these cells. All three enzymes are present in microvillus plasma membranes, rough endoplasmic reticulum, cytoplasm, nuclear membranes and chromatin. In summary, 5- and 12-lipoxygenases and cyclooxygenase enzymes, which catalyze the transformation of arachidonic acid into different eicosanoids, are present in several subcellular organelles including nuclei of granulosa cells from preovulatory follicles.
authors.affiliation: Department of Obstetrics and Gynecology, University of Louisville School of Medicine, Kentucky 40292.
authors.forename: D
authors.initials: D
authors.lastname: Pridham
authors.forename: Z M
authors.initials: ZM
authors.lastname: Lei
authors.forename: N
authors.initials: N
authors.lastname: Chegini
authors.forename: C V
authors.initials: CV
authors.lastname: Rao
authors.forename: M A
authors.initials: MA
authors.lastname: Yussman
authors.forename: C L
authors.initials: CL
authors.lastname: Cook
mesh_terms: D001092:Arachidonate 12-Lipoxygenase / Q000032:analysis*; D001094:Arachidonate 5-Lipoxygenase / Q000032:analysis*; D001091:Arachidonate Lipoxygenases / Q000032:analysis*; D005260:Female; D005498:Follicular Phase; D006107:Granulosa Cells / Q000201:enzymology* / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009928:Organ Specificity; D011451:Prostaglandin-Endoperoxide Synthases / Q000032:analysis*
chemical_list: D001091:Arachidonate Lipoxygenases; D001092:Arachidonate 12-Lipoxygenase; D001094:Arachidonate 5-Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases