title: Different calcium requirements during incorporation of myo-inositol into membrane lipids for phosphatidylinositol hydrolysis stimulated by carbachol and noradrenaline.
abstract: Stimulation of phosphatidylinositol hydrolysis by noradrenaline requires the presence of calcium in the medium during the whole incubation and preincubation procedure, while carbachol-induced inositol phosphate formation is equally efficient in calcium-free and calcium-containing medium. This finding confirms the notion that phosphatidylinositols mobilized after stimulation of various receptors are present in different metabolic pools: the pool mobilized by alpha 1 adrenoceptors is apparently formed from myo-inositol incorporated into membrane phospholipids by a calcium-dependent process.
authors.affiliation: Department of Biochemistry, Polish Academy of Sciences, Kraków.
authors.forename: I
authors.initials: I
authors.lastname: Nalepa
authors.forename: E
authors.initials: E
authors.lastname: Chalecka-Franaszek
authors.forename: J
authors.initials: J
authors.lastname: Vetulani
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002118:Calcium / Q000502:physiology*; D002217:Carbachol / Q000494:pharmacology*; D006868:Hydrolysis / Q000187:drug effects; D007294:Inositol / Q000378:metabolism*; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology*; D010716:Phosphatidylinositols / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011941:Receptors, Adrenergic / Q000187:drug effects / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000378:metabolism
chemical_list: D008563:Membrane Lipids; D010716:Phosphatidylinositols; D011941:Receptors, Adrenergic; D011976:Receptors, Muscarinic; D007294:Inositol; D002217:Carbachol; D002118:Calcium; D009638:Norepinephrine