Blood screening for non-A, non-B hepatitis by hepatitis C virus antibody assay.
Hepatitis C virus (HCV) antibody was detected in 1499 donor sera by radioimmunoassay using an antigen expressed in yeast from a cDNA clone of the HCV genome. Eighteen samples over 4200 counts per minute (cpm) were considered to contain infectious HCV because these recipients developed typical posttransfusion non-A, non-B hepatitis after transfusion. The antibody-positive sera were all within the normal range of ALT levels. This assay system is thus useful for the screening for blood transfusion.
Katayama
T
T
Department of Surgery, Tokyo National Chest Hospital, Japan.
Kikuchi
S
S
Tanaka
Y
Y
Saito
I
I
Miyamura
T
T
Choo
Q L
QL
Houghton
M
M
Kuo
G
G
D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000032:analysis*; D005260:Female; D006526:Hepatitis C / Q000382:microbiology / Q000517:prevention & control*; D006518:Hepatitis Viruses / Q000276:immunology; D006525:Hepatitis, Viral, Human / Q000517:prevention & control*; D006801:Humans; D008297:Male; D008403:Mass Screening; D008875:Middle Aged; D011863:Radioimmunoassay / Q000379:methods; D011994:Recombinant Proteins / Q000032:analysis
D000914:Antibodies, Viral; D011994:Recombinant Proteins