[Effects of sporidesmin on liver zinc, metallothionein and cytochromes in vivo].
In vitro studies have suggested that sporidesmin hepatotoxicity may be related to thiol oxidation and generation of cytotoxic oxygen species. After a single i.p. injection of 2.8 mg/kg bw sporidesmin in guinea-pigs, hepatic and plasma zinc, hepatic metallothionein, cytochromes P-450 and b5, total glutathione and proteins (total, microsomal and cytosolic) were monitored for 21 days. The only variations observed were significant increases in liver concentrations of zinc (cytosolic and total), metallothionein, and cytochromes, which peaked on day 8 after the sporidesmin challenge (+45, 55, 50, 376 and 413%, respectively) and, except for cytochrome b5, went back to control levels before the 21st day. These results suggest that cytochromes P-450 and b5 may be involved in sporidesmin cellular damage.
Bonnefoi
M
M
Laboratoire associée I.N.R.A. de Toxicologie biochimique et métabolique, Ecole nationale vétérinaire de Toulouse.
Hasim
M
M
Sauvagnac
P
P
de Saqui-Sannes
P
P
Rico
A
A
Burgat
V
V
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis*; D015786:Cytochromes b5 / Q000032:analysis*; D006168:Guinea Pigs; D007274:Injections, Intraperitoneal; D008099:Liver / Q000737:chemistry*; D008297:Male; D008668:Metallothionein / Q000032:analysis*; D013173:Sporidesmins / Q000008:administration & dosage / Q000494:pharmacology*; D015032:Zinc / Q000032:analysis*
D013173:Sporidesmins; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; D008668:Metallothionein; D015032:Zinc
Effets de la sporidesmine sur le zinc, la métallothionéine et les cytochromes hépatiques in vivo.