title: ADP-ribosylation of nuclear proteins in rat ventral prostate during ageing.
abstract: Poly(ADPR)polymerase activity and poly(ADP-ribosyl)ation of nuclear proteins have been investigated in ventral prostate nuclei of different aged rats (14, 28, 60, 180, 360 day old animals), by reverse-phase HPLC and acetic acid-urea polyacrylamide gel electrophoresis. The major ADP-ribose acceptor proteins were identified as histone H1 and H2b. It is concluded that concomitant with major changes to chromatin organization, poly(ADP-ribosyl)ation reaction is progressively inhibited during aging of rat ventral prostate. These results support the hypothesis that prostatic dysfunction in senescent animals is related to a failure of DNA repair mechanisms and deregulated template activity.
authors.affiliation: Dipartimento di Chimica Organica e Biologica, Università di Napoli, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Quesada
authors.forename: M R
authors.initials: MR
authors.lastname: Faraone-Mennella
authors.forename: R
authors.initials: R
authors.lastname: Jones
authors.forename: M
authors.initials: M
authors.lastname: Malanga
authors.forename: B
authors.initials: B
authors.lastname: Farina
mesh_terms: D000375:Aging; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D002843:Chromatin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004260:DNA Repair; D008297:Male; D009687:Nuclear Proteins / Q000378:metabolism*; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism*; D011467:Prostate / Q000201:enzymology* / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000596:Amino Acids; D002843:Chromatin; D009687:Nuclear Proteins; D011065:Poly(ADP-ribose) Polymerases