title: Effect of environmental conditions on production of toxic shock syndrome toxin 1 by Staphylococcus aureus.
abstract: The kinetics of toxic shock syndrome toxin 1 (TSST-1) production by Staphylococcus aureus was studied in a fermentor in which aeration rate, atmospheric composition, pH, and temperature were controlled. The toxin was synthesized at a maximal rate during the exponential phase. High bacterial populations were not necessarily accompanied by high TSST-1 yields. Aerobiosis increased TSST-1 production, but excessive aeration had an adverse effect. Addition of CO2 enhanced TSST-1 yield by increasing toxin production rate and efficiency. Cultures with no pH control made more TSST-1 than those maintained at pH 5.5 to 7.5. Maximum TSST-1 yields were obtained when cultures were supplied with air (20 cm3/min) and CO2 (5 cm3/min) via a sintered glass sparger.
authors.affiliation: Food Research Institute, University of Wisconsin, Madison 53706.
authors.forename: A C
authors.initials: AC
authors.lastname: Wong
authors.forename: M S
authors.initials: MS
authors.lastname: Bergdoll
mesh_terms: D001427:Bacterial Toxins; D002245:Carbon Dioxide / Q000494:pharmacology; D003470:Culture Media; D004768:Enterotoxins / Q000096:biosynthesis*; D010100:Oxygen / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000378:metabolism*; D018089:Superantigens
chemical_list: D001427:Bacterial Toxins; D003470:Culture Media; D004768:Enterotoxins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal; D002245:Carbon Dioxide; D010100:Oxygen