Rat granulosa cells express transforming growth factor-beta type 2 messenger ribonucleic acid which is regulatable by follicle-stimulating hormone in vitro.
Freshly harvested granulosa cells (GC) from diethylstilbestrol (DES)-treated rats were examined for the presence of transforming growth factor-beta 1 (TGF-beta 1) and TGF-beta 2 mRNA by Northern analysis. TGF-beta 1 mRNA was not detectable, but hybridization of total RNA with a radiolabeled TGF-beta 2 cDNA probe revealed two mRNA species (5.1 and 3.6 kb) indicative of TGF-beta 2 mRNA. In response to FSH (50 ng/ml), relative TGF-beta 2 mRNA concentrations in cultured GC were 54% of control levels. Furthermore, the conditioned culture medium from FSH-treated GC contained significantly lower (p less than 0.01) TGF-beta-like activity relative to controls. These results demonstrate that rat GC express TGF-beta 2 mRNA which is regulatable by FSH in vitro.
Mulheron
G W
GW
Department of Obstetrics/Gynecology, Duke University Medical Center, Durham, NC 27710.
Schomberg
D W
DW
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D004247:DNA; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D015291:Transforming Growth Factors / Q000145:classification / Q000235:genetics*; D014407:Tumor Cells, Cultured / Q000378:metabolism
D003470:Culture Media; D012333:RNA, Messenger; D015291:Transforming Growth Factors; D005640:Follicle Stimulating Hormone; D004247:DNA