Inhibition of DNA excision by DNA polymerase-alpha inhibitor in UV-damaged HeLa cells.
HeLa cells treated with 10 J/m2 of ultraviolet (UV) radiation were examined for inhibition of DNA excision by inhibitors of DNA polymerase-alpha and -beta. DNA repair synthesis and excision were inhibited by aphidicolin, a specific inhibitor of DNA polymerase-alpha. Decreased release of radioactive nucleotides from UV-damaged DNA by the inhibitor indicates that the action of DNA polymerase-alpha activity is closely associated with DNA excision. Dideoxythymidine also inhibited DNA repair synthesis but failed to block the excision, suggesting that polymerization by DNA polymerase-beta might precede removal of damaged DNA.
Joe
C O
CO
Department of Biology, Korea Institute of Technology, Taejon.
Lee
M A
MA
Choi
B S
BS
Kang
K W
KW
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D016590:Aphidicolin; D002478:Cells, Cultured; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors*; D004260:DNA Repair / Q000187:drug effects*; D015224:Dideoxynucleosides / Q000494:pharmacology*; D004224:Diterpenes / Q000494:pharmacology*; D006367:HeLa Cells / Q000187:drug effects* / Q000201:enzymology / Q000528:radiation effects; D006801:Humans; D014466:Ultraviolet Rays / Q000009:adverse effects
D000903:Antibiotics, Antineoplastic; D015224:Dideoxynucleosides; D004224:Diterpenes; C019336:2',3'-dideoxythymidine; D016590:Aphidicolin; D004257:DNA Polymerase II