Inflammatory mediators in the vitreous humor of AIDS patients with retinitis.
We measured levels of protein, of complement-derived anaphylatoxins (C3a, C4a, and C5a), and of the lymphokines interleukin-2 and gamma interferon, in vitreous humor from 10 AIDS patients with vitritis and retinitis (group 1). We compared these measurements with levels in vitreous from 7 patients with vitritis but without AIDS (group 2), 10 patients with vitreous hemorrhages (group 3), and 20 patients with retinal detachments or epiretinal membranes without clinical evidence of vitreal inflammation (group 4). Vitreous humor from 10 AIDS patients had measurable levels of interleukin-2 in three of nine samples, gamma interferon in six of nine samples, C3a and C4a in all ten samples, and C5a in only one of ten samples. Vitreous humor from group 1 did not differ significantly from vitreous from group 2. On the other hand, vitreous from group 1 had significantly higher levels of gamma interferon, C3a, and C4a, and higher ratios of these anaphylatoxins to protein, in comparison to vitreous in groups 3 and 4. The results of this study suggest that vitreous humor from AIDS patients with retinitis contains activated complement and may contain interleukin-2 and gamma interferon. Viral retinitis is associated with the presence of lymphokines in vitreous humor. Additionally, anaphylatoxin and gamma interferon levels, but not interleukin-2 levels, correlate with vitreal inflammation. This is the first study to measure interleukin-2, gamma interferon, and C5a levels in human vitreous humor.
Mondino
B J
BJ
Uveitis Center, Jules Stein Eye Institute, University of California-Los Angeles School of Medicine 90024-1771.
Sidikaro
Y
Y
Mayer
F J
FJ
Sumner
H L
HL
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications / Q000276:immunology*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D000706:Anaphylatoxins / Q000378:metabolism; D003165:Complement System Proteins / Q000378:metabolism; D005128:Eye Diseases / Q000276:immunology; D005136:Eye Proteins / Q000378:metabolism; D005260:Female; D006801:Humans; D007249:Inflammation / Q000276:immunology; D007371:Interferon-gamma / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D008297:Male; D011863:Radioimmunoassay; D012173:Retinitis / Q000150:complications / Q000276:immunology*; D014822:Vitreous Body / Q000276:immunology*
D000706:Anaphylatoxins; D005136:Eye Proteins; D007376:Interleukin-2; D007371:Interferon-gamma; D003165:Complement System Proteins