Mutational mapping of RAS-responsive domains of the Saccharomyces cerevisiae adenylyl cyclase.
Large deletion and small insertion mutations in the adenylyl cyclase gene of Saccharomyces cerevisiae were used to map regions required for activation by RAS protein in vitro. The amino-terminal 605 amino acids were found to be dispensable for responsiveness to RAS protein. All other deletions in adenylyl cyclase destroyed its ability to respond to RAS. Small insertion mutations within the leucine-rich repeat region also prevented RAS responsiveness, while other insertions did not.
Colicelli
J
J
Cold Spring Harbor Laboratory, New York 11724.
Field
J
J
Ballester
R
R
Chester
N
N
Young
D
D
Wigler
M
M
D000262:Adenylyl Cyclases / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D019204:GTP-Binding Proteins / Q000235:genetics*; D005800:Genes, Fungal; D008970:Molecular Weight; D009154:Mutation; D010957:Plasmids; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D018631:ras Proteins
D004271:DNA, Fungal; D005656:Fungal Proteins; D019204:GTP-Binding Proteins; D018631:ras Proteins; D000262:Adenylyl Cyclases