Evidence of a growth hormone-releasing hormone-like molecule in salmon brain, Oncorhynchus keta and O. kisutch.
An antiserum with conformational specificity to human growth hormone-releasing hormone (hGH-RH 1-44 NH2) was produced and used to develop a radioimmunoassay to detect immunoreactive (ir) GH-RH in brain extracts of salmon, guinea pig, and mouse. Evidence of an immunoreactive GH-RH from salmon brain extracts with a retention time on reverse-phase high-performance liquid chromatography (HPLC) distinct from hGH-RH 1-44 is presented. Salmon irGH-RH from crude extracts elutes 1-2 min earlier than hGH-RH 1-44 (NH2) in a gradient HPLC system, whereas affinity-purified salmon irGH-RH elutes 12-13 min earlier in a near-isocratic system, suggesting that the salmon molecule is more hydrophilic.
Parker
D B
DB
Department of Biology, University of Victoria, British Columbia, Canada.
Sherwood
N M
NM
D000818:Animals; D000918:Antibody Specificity; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D013007:Growth Hormone-Releasing Hormone / Q000031:analogs & derivatives / Q000032:analysis* / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology; D011863:Radioimmunoassay; D012474:Salmon / Q000378:metabolism*
D007106:Immune Sera; D010446:Peptide Fragments; C040786:somatotropin releasing hormone (1-44)amide; D013007:Growth Hormone-Releasing Hormone