[Comparative study with digoxigenin and biotin markers for the DNA probe identification of genes coding for type TEM beta-lactamases].
We have tested two non-isotopic labels: digoxigenin--11-dUTP and biotin-7-dATP for the detection of TEM-1 beta-lactamase gene with a TEM-1 probe by DNA:DNA hybridisation (using spot technique). The use of radioactive labels is inconvenient and not available to all the clinical bacteriological laboratories. The strains tested were: 16 on file (14 carriers of different types of beta-lactamase) and 19 clinical isolated strains. Previously, the plasmid beta-lactamase of these strains had been identified by analytical isoelectric focusing. Using the probe labeled with digoxigenin-11-dUTP, the correlation between results obtained by DNA:DNA hybridisation and those by analytical isoelectric focusing was excellent, with no false positives; with the biotin--7-dATP labeled probe, reading was difficult and some false positives were observed.
Hermida
M
M
Facultad de Medicina (Departamento de Genética y Microbiología), Universidad Autónoma de Barcelona.
Gibert
I
I
Barbé
J
J
Roy
C
C
D001710:Biotin; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D004076:Digoxigenin; D006090:Gram-Negative Bacteria / Q000201:enzymology / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D001618:beta-Lactamases / Q000235:genetics*
D015342:DNA Probes; D004269:DNA, Bacterial; D001710:Biotin; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1; D004076:Digoxigenin
Estudio comparativo con los marcadores digoxigenina y biotina para la identificación mediante sondas de ADN de los genes que codifican betalactamasas de tipo TEM.