Copy choice mechanism of immunoglobulin heavy-chain switch recombination.
The immunoglobulin heavy-chain switch is mediated by a recombination event between DNA switch regions associated with donor and recipient constant-region genes. We have determined that the mutations which can be found in some switch regions after recombination appear to arise on only one strand of DNA. This result suggests that switch recombination involves error-prone synthesis of one DNA strand and ligation of the other strand from preexisting DNA.
Dunnick
W
W
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109-0620.
Stavnezer
J
J
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000096:biosynthesis; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D005803:Genes, Immunoglobulin; D005809:Genes, Regulator; D005812:Genes, Switch; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D015183:Restriction Mapping
D007143:Immunoglobulin Heavy Chains; D004247:DNA