Increased levels of 5-fluorouracil-induced DNA lesions in Bloom's syndrome.
In Bloom's syndrome (BS) the regulation of uracil-DNA glycosylase, an enzyme involved in the repair of DNA containing 5-FU, is altered. 5-FU induces higher levels of DNA fragmentation in BS cells than in non-BS cells. The increase in DNA fragmentation is connected to the cytotoxic mechanism where 5-FU is incorporated into DNA. When 5-FU induces DNA fragmentation by a mechanism not involving the incorporation of drug into DNA, the levels of DNA fragmentation in BS and non-BS cells remain similar.
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Radiumhemmet, Karolinska Hospital, Stockholm, Sweden.
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Winblad
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D016590:Aphidicolin; D001816:Bloom Syndrome / Q000201:enzymology / Q000235:genetics*; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D045647:DNA Glycosylases; D004260:DNA Repair / Q000187:drug effects*; D004224:Diterpenes / Q000494:pharmacology; D005472:Fluorouracil / Q000494:pharmacology*; D006801:Humans; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D013449:Sulfonamides / Q000494:pharmacology; D051981:Uracil-DNA Glycosidase
D004224:Diterpenes; D013449:Sulfonamides; D016590:Aphidicolin; C017967:W 7; D004247:DNA; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase; D005472:Fluorouracil