title: Experimental ovine salmonellosis (Salmonella abortusovis): pathogenesis and vaccination.
abstract: Salmonella enterica subsp. enterica ser. Abortusovis, a sheep-adapted serotype, causes a contagious disease. Abortion is the major symptom and the main source of contamination. Research on this ovine disease may aid farmers, but may also contribute to comparative biological knowledge. Innate resistance partly controlled by the Ity locus, increased resistance to reinfection and humoral and T-cell-mediated immunity were observations gained with a murine model. In ewes, abortion regularly occurs following subcutaneous challenge carried out from the third month of gestation onwards. This ovine model was used to evaluate prevention methods for Salmonella Abortusovis infection. One subcutaneous injection of a live attenuated lyophilized vaccine containing a selected streptomycin-independent reverse mutant was shown to protect ewes against abortion and excretion of Salmonella Abortusovis. This vaccine could be administered simultaneously with other commercial live vaccines such as Brucella melitensis Rev. 1 vaccine. In sheep, application of the vaccine to the conjunctiva (an easy, individual and hygienic route of mucosal vaccination) was followed by lymph node bacterial colonization and a serological response without local or general clinical reactions. The early events of natural infection remain to be explored, as do the mechanisms underlying the host specificity of Salmonella Abortusovis.
authors.affiliation: Institut National de la Recherche Agronomique, Laboratoire de Pathologie infectieuse et Immunologie, Nouzilly, France.
authors.forename: P
authors.initials: P
authors.lastname: Pardon
authors.forename: R
authors.initials: R
authors.lastname: Sanchis
authors.forename: J
authors.initials: J
authors.lastname: Marly
authors.forename: F
authors.initials: F
authors.lastname: Lantier
authors.forename: L
authors.initials: L
authors.lastname: Guilloteau
authors.forename: D
authors.initials: D
authors.lastname: Buzoni-Gatel
authors.forename: I P
authors.initials: IP
authors.lastname: Oswald
authors.forename: M
authors.initials: M
authors.lastname: Pépin
authors.forename: B
authors.initials: B
authors.lastname: Kaeffer
authors.forename: P
authors.initials: P
authors.lastname: Berthon
mesh_terms: D000034:Abortion, Veterinary / Q000209:etiology / Q000276:immunology / Q000517:prevention & control; D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000302:isolation & purification; D004195:Disease Models, Animal; D005260:Female; D011247:Pregnancy; D012475:Salmonella / Q000276:immunology; D012481:Salmonella Infections, Animal / Q000209:etiology* / Q000276:immunology / Q000517:prevention & control; D012756:Sheep; D012757:Sheep Diseases / Q000209:etiology* / Q000276:immunology / Q000517:prevention & control; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000302:isolation & purification
chemical_list: D001428:Bacterial Vaccines; D014613:Vaccines, Attenuated