Characterization of the wheat mitochondrial orf25 gene.
The wheat mitochondrial orf25 nucleotide sequence of 576 bp has been determined. Its derived protein sequence shares 88% and 75% amino acid identity with those of maize and tobacco mitochondria, respectively. The wheat and tobacco orf25 sequences lack four inserts, of 6 bp to 36 bp, that are present in the maize homologue. The wheat orf25 gene is actively transcribed and is preceded by a regulatory sequence block very similar to those located upstream of the wheat coxII and atp6 genes. Our observations support the view that orf25 sequences encode a functional polypeptide in plant mitochondria.
Bonen
L
L
Department of Biology, University of Ottawa, Canada.
Bird
S
S
Belanger
L
L
D000595:Amino Acid Sequence; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics*; D017343:Genes, Plant; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010940:Plant Proteins / Q000235:genetics*; D010947:Plants, Toxic; D016415:Sequence Alignment; D014026:Nicotiana / Q000235:genetics; D014908:Triticum / Q000235:genetics* / Q000648:ultrastructure; D003313:Zea mays / Q000235:genetics
D004272:DNA, Mitochondrial; D010940:Plant Proteins