Regulation of the Escherichia coli glyA gene by the purR gene product.
The purine regulon repressor protein, PurR, was shown to be a purine component involved in glyA regulation in Escherichia coli. Expression of glyA, encoding serine hydroxymethyltransferase activity, was elevated in a purR mutant compared with a wild-type strain. When the purR mutant was transformed with a plasmid carrying the purR gene, the serine hydroxymethyltransferase levels returned to the wild-type level. The PurR protein bound specifically to a DNA fragment carrying the glyA control region, as determined by gel retardation. In a DNase I protection assay, a 24-base-pair region was protected from DNase I digestion by PurR. The glyA operator sequence for PurR binding is similar to that reported for several pur regulon genes.
Steiert
J G
JG
Department of Microbiology, University of Iowa, Iowa City 52242.
Rolfes
R J
RJ
Zalkin
H
H
Stauffer
G V
GV
D001483:Base Sequence; D001665:Binding Sites; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D003850:Deoxyribonuclease I; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005998:Glycine / Q000096:biosynthesis*; D012696:Glycine Hydroxymethyltransferase / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D012694:Serine / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*; D014161:Transduction, Genetic; D014166:Transferases / Q000235:genetics*
D004269:DNA, Bacterial; D012097:Repressor Proteins; D014157:Transcription Factors; D012694:Serine; D014166:Transferases; D012696:Glycine Hydroxymethyltransferase; D003850:Deoxyribonuclease I; D005998:Glycine