Indirect inhibition of generation of murine lymphokine-activated killer cell activity in splenocyte cultures by interferon-gamma.
The effect of mouse recombinant interferon-gamma (rIFN-gamma) on murine lymphokine-activated killer (LAK) cell activity was investigated using natural killer (NK)-resistant, spontaneously developed, weakly immunogenic and highly tumourigenic, syngeneic murine mammary adenocarcinoma, JC, mimicking that of human disease, as the target. Murine YAC-1 also was used as a target cell line. rIFN-gamma, when used in combination with recombinant interleukin-2 (rIL-2), was shown to exhibit a suppressed effect on LAK cell activity generated from BALB/c mouse splenocytes, compared to that with rIL-2 alone. The decrease in LAK cell activity was rIFN-gamma dose-dependent. Kinetic study revealed that this inhibitory effect was demonstrated only when rIFN-gamma was added to the medium at the early phase of rIL-2 culture. The inhibitory effect on LAK cell generation by rIFN-gamma was completely abrogated when the nylon-wool-treated non-adherent 'macrophage-free' splenocytes were incubated with rIL-2 and rIFN-gamma. These results indicated that the LAK cell activity generated from murine splenocytes cultured with rIL-2 could be depressed by rIFN-gamma, and that the macrophages may be involved as mediators.
Chao
T Y
TY
Department of Diagnostic Immunology Research, Roswell Park Memorial Institute, Buffalo, NY 14263.
Ohnishi
H
H
Chu
T M
TM
D000818:Animals; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D007371:Interferon-gamma / Q000494:pharmacology*; D015979:Killer Cells, Lymphokine-Activated / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins; D013154:Spleen / Q000187:drug effects / Q000276:immunology*
D011994:Recombinant Proteins; D007371:Interferon-gamma