title: Ciliary microtubule capping structures contain a mammalian kinetochore antigen.
abstract: Structures that cap the plus ends of microtubules may be involved in the regulation of their assembly and disassembly. Growing and disassembling microtubules in the mitotic apparatus are capped by kinetochores and ciliary and flagellar microtubules are capped by the central microtubule cap and distal filaments. To compare the ciliary caps with kinetochores, isolated Tetrahymena cilia were stained with CREST (Calcinosis/phenomenon esophageal dysmotility, sclerodactyly, telangiectasia) antisera known to stain kinetochores. Immunofluorescence microscopy revealed that a CREST antiserum stained the distal tips of cilia that contained capping structures but did not stain axonemes that lacked capping structures. Both Coomassie blue-stained gels and Western blots probed with CREST antiserum revealed that a 97-kD antigen copurifies with the capping structures. Affinity-purified antibodies to the 97-kD ciliary protein stained the tips of cap-containing Tetrahymena cilia and the kinetochores in HeLa, Chinese hamster ovary, and Indian muntjak cells. These results suggest that at least one polypeptide found in the kinetochore is present in ciliary microtubule capping structures and that there may be a structural and/or functional homology between these structures that cap the plus ends of microtubules.
authors.affiliation: Department of Physiology and Cell Biology, University of Kansas, Lawrence 66045-2106.
authors.forename: J M
authors.initials: JM
authors.lastname: Miller
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.forename: R
authors.initials: R
authors.lastname: Balczon
authors.forename: W L
authors.initials: WL
authors.lastname: Dentler
mesh_terms: D000818:Animals; D000941:Antigens / Q000032:analysis*; D002923:Cilia / Q000648:ultrastructure*; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D006367:HeLa Cells / Q000648:ultrastructure; D006801:Humans; D007106:Immune Sera; D015151:Immunoblotting; D008854:Microscopy, Electron; D008870:Microtubules / Q000648:ultrastructure*; D008961:Models, Structural; D015388:Organelles / Q000648:ultrastructure*; D013768:Tetrahymena / Q000648:ultrastructure*; D013861:Thiocyanates
chemical_list: D000941:Antigens; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007106:Immune Sera; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate