Expression of the yeast LEU4 gene is subject to four different modes of control.
A translational fusion of yeast LEU4 and Escherichia coli lacZ which contains 679 bp of the LEU4 5'-flanking region and the first two codons of LEU4 was used to study LEU4 expression. Eight recipient strains with different genetic backgrounds, transformed with a plasmid containing the fusion, were grown under a variety of conditions, and beta-galactosidase activity was measured. Evidence was obtained for at least four modes of expression of LEU4: general amino acid control, leucine-specific control, basal level expression, and branched-chain amino acid-mediated repression. Determination of steady-state levels of LEU4 mRNA suggested that LEU4 expression is regulated transcriptionally.
Peters
M H
MH
Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907.
Beltzer
J P
JP
Kohlhaw
G B
GB
D015079:2-Isopropylmalate Synthase / Q000235:genetics*; D001483:Base Sequence; D003062:Codon; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005838:Genotype; D007930:Leucine / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D007652:Oxo-Acid-Lyases / Q000235:genetics*; D010957:Plasmids; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D003062:Codon; D015345:Oligonucleotide Probes; D011994:Recombinant Proteins; D015079:2-Isopropylmalate Synthase; D001616:beta-Galactosidase; D007652:Oxo-Acid-Lyases; D007930:Leucine