Activation of the serum-response and TPA-response elements by expression of the v-abl protein: comparison of the mode of action of the v-abl protein with those of protein kinase C, cyclic AMP-dependent protein kinase, and the activated c-raf protein.
In the present study, we have compared the mode of action of the v-abl protein in the regulation of gene expression with those of serine/threonine protein kinases such as protein kinase C, cyclic AMP-dependent protein kinase, and the activated c-raf protein, by measuring the transcriptional activity of the serum-response element, the 12-O-tetradecanoylphorbol-13-acetate (TPA)-response element, and the cyclic AMP-response element in NIH3T3 cells transfected with the v-abl gene. The results indicate that the v-abl protein stimulates the serum-response element and the TPA-response element, but not the cyclic AMP-response element, in a manner similar to that of the activated c-raf protein, but different from those of protein kinase C and cyclic AMP-dependent protein kinase.
Hori
Y
Y
Department of Biochemistry, Kobe University School of Medicine, Japan.
Kaibuchi
K
K
Fukumoto
Y
Y
Oku
N
N
Takai
Y
Y
D001790:Blood Physiological Phenomena; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D015536:Down-Regulation; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005786:Gene Expression Regulation; D008156:Luciferases / Q000235:genetics; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011493:Protein Kinase C / Q000502:physiology*; D011494:Protein Kinases / Q000502:physiology*; D011518:Proto-Oncogene Proteins / Q000235:genetics; D016760:Proto-Oncogene Proteins c-fos; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics*
D010982:Platelet-Derived Growth Factor; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D014764:Viral Proteins; D004815:Epidermal Growth Factor; D008156:Luciferases; D015500:Chloramphenicol O-Acetyltransferase; D011494:Protein Kinases; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate