Immunological detection of mycobacterial antigens in infected fluids, cells and tissues by latex agglutination. Animal model and clinical application.
We devised an immunoassay for the detection of mycobacterial antigens in cell lysates and in tissue extracts which is based on the agglutination of latex particles coated with anti-Mycobacterium bovis F(ab')2, followed by counting of non-agglutinated particles. Mycobacterium bovis cell lysates were tested and a reference curve was established, having a lower limit of detection of 15-20 Mycobacteria. We were able to detect mycobacterial antigens in cell lysates from bronchoalveolar washings and in spleen and liver lysates obtained from experimentally infected rabbits. Antigens were also detected in ten out of 11 samples obtained from patients with proven tuberculous infection. These samples were readily distinguished from 32 negative control samples after pepsin treatment. In contrast, periodate treatment of samples to destroy carbohydrate, abolished all reactivity. Following gel filtration chromatography we identified three peaks with antigenic properties in samples of all types. The detection of mycobacterial carbohydrate antigens by latex agglutination and particle counting should be a useful adjunct in the diagnosis of tuberculosis.
Cambiaso
C L
CL
Unit of Experimental Medicine, Université Catholique de Louvain, Bruxelles, Belgium.
Van Vooren
J P
JP
Farber
C M
CM
D000372:Agglutination Tests / Q000379:methods*; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D002850:Chromatography, Gel; D004195:Disease Models, Animal; D006801:Humans; D008863:Microspheres; D009164:Mycobacterium Infections / Q000175:diagnosis*; D009163:Mycobacterium bovis / Q000276:immunology*; D010434:Pepsin A; D010996:Pleural Effusion / Q000276:immunology; D011817:Rabbits; D012015:Reference Standards; D013582:Synovial Fluid / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*
D000942:Antigens, Bacterial; D010434:Pepsin A