title: Cloning and nucleotide sequence of the crtI gene encoding phytoene dehydrogenase from the cyanobacterium Aphanocapsa PCC6714.
abstract: The membrane-bound phytoene dehydrogenase (PD) is an enzyme in carotenoid biosynthesis which is essential in all microorganisms and plants containing these colored pigments. Despite its key role in the regulation of carotenogenesis, the biochemistry and molecular biology of PD are poorly understood. We have cloned, sequenced and expressed a portion of the PD-encoding gene, crtI, from the blue-green algae Aphanocapsa PCC6714. The gene codes for a 532-amino acids (aa) protein, with a calculated Mr of 52,598 Da. Two regions of the aa sequence share significant homology with PD from the purple bacterium Rhodobacter capsulatus, including a 30-aa region which has been proposed to be specific for dehydrogenases in carotenoid biosynthesis.
authors.affiliation: Lehrstuhl für Biochemie und Physiologie der Pflanzen, Universität Konstanz, F.R.G.
authors.forename: A
authors.initials: A
authors.lastname: Schmidt
authors.forename: G
authors.initials: G
authors.lastname: Sandmann
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000201:enzymology / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics* / Q000302:isolation & purification; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid
chemical_list: D011993:Recombinant Fusion Proteins; D010088:Oxidoreductases; C022398:phytoene dehydrogenase