Differential reactivity of bovine lymphocytes to species of Brucella.
The reactivity of bovine lymphocytes to 4 species of Brucella was tested in thymidine-uptake assays, using long-term cultured lymphocytes and freshly obtained blood mononuclear cells. Lymphocytes were taken from cows that had been challenge exposed with a virulent strain of B abortus at midgestation. The cows were classified retrospectively as being naturally resistant or susceptible to brucellosis. Lymphocytes taken from these cows had 3 patterns of reactivity with species of Brucella: pattern 1 was defined by reactivity with 4 species (B abortus, B canis, B suis, and B melitensis); pattern 2 was defined by reactivity with all these species, except B melitensis; pattern 3 was defined by reactivity with B abortus and B canis, but not with B suis or B melitensis. There was a statistically significant correlation between susceptibility to brucellosis and expression of lymphocyte cross-reactivity with B suis (P less than 0.01) and with B melitensis (P less than 0.001).
Smith
R
R
Department of Veterinary Pathology, Texas A&M University, College Station 77843-4463.
Kapatsa
J C
JC
Sherwood
S J
SJ
Ficht
T A
TA
Templeton
J W
JW
Adams
L G
LG
D000818:Animals; D002003:Brucella abortus / Q000276:immunology* / Q000302:isolation & purification; D002417:Cattle / Q000097:blood / Q000276:immunology*; D002460:Cell Line; D005260:Female; D008214:Lymphocytes / Q000276:immunology*; D013045:Species Specificity; D013936:Thymidine
D013936:Thymidine