title: [Phosphorylation of tryptophanyl-tRNA-synthetase by casein kinase type II].
abstract: Incubation of tryptophanyl-tRNA synthetase from bovine pancrease with [gamma-32P]ATP of [gamma-32P]GTP and casein kinase II from rabbit liver leads to the incorporation of labeled phosphate into serine residues of synthetase polypeptide. The maximal level of 32P incorporation into synthetase polypeptide (Mr = 60 kDa) 0.15 moles of 32P per 1 mole of polypeptide was observed. Electrophoretic analysis according to O'Farrell showed that kinase phosphorylates exclusively the most acidic polypeptides (pI 4.9) of the synthetase preparation. Pretreatment of synthetase with animal acidic and alkaline phosphatases had no influence on the level of 32P incorporation in synthetase during subsequent incubation in the presence of casein kinase II.
authors.forename: S M
authors.initials: SM
authors.lastname: Elizarov
authors.forename: G K
authors.initials: GK
authors.lastname: Kovaleva
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D047388:Casein Kinases; D002417:Cattle; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000201:enzymology; D010179:Pancreas / Q000201:enzymology; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D011817:Rabbits; D014369:Tryptophan-tRNA Ligase / Q000378:metabolism*
chemical_list: D011494:Protein Kinases; D047388:Casein Kinases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D014369:Tryptophan-tRNA Ligase
vernacular_title: Fosforilirovanie triptofanil-tRNK-sintetazy kazeinovoĭ kinazoĭ tipa II.