Tumor necrosis factor alpha and interferon gamma inhibit proliferation and alkaline phosphatase activity of human osteoblastic SaOS-2 cell line.
Tumor necrosis factor (TNF) alpha and TNF beta both inhibited proliferation of cultured human osteoblastic SaOS-2 cells. TNF alpha also inhibited alkaline phosphatase (ALP) activity in the cells. The TNF alpha-induced inhibition of proliferation and ALP activity was further potentiated by interferon (IFN) gamma. These findings indicate that human SaOS-2 cells, fulfilling several criteria for osteoblasts, respond to TNF alpha and IFN gamma, resulting in decceleration of their maturation.
Yoshihara
R
R
Department of Medicine, Kobe University, Japan.
Shiozawa
S
S
Imai
Y
Y
Fujita
T
T
D000469:Alkaline Phosphatase / Q000378:metabolism*; D002455:Cell Division; D004357:Drug Synergism; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D010006:Osteoblasts / Q000201:enzymology* / Q000473:pathology; D012516:Osteosarcoma; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D000469:Alkaline Phosphatase