Control of production of insulin-like growth factor I by pig Leydig and Sertoli cells cultured alone or together. Cell-cell interactions.
The production of insulin-like growth factor I (IGF-I) by pig Leydig cells and pig Sertoli cells cultured alone or together was investigated. Human chorionic gonadotropin (hCG) and basic fibroblast growth factor (FGF) stimulate in a dose-dependent manner IGF-I production by Leydig cells. At maximal concentrations the effects of both factors were almost additive. Insulin at micromolar concentrations enhanced IGF-I production and potentiated the effects of hCG and FGF. The secretion of IGF-I by Sertoli cells was stimulated by FSH and FGF. Under basal conditions, the production of IGF-I by the coculture was similar to the addition of the production by each cell cultured alone. In contrast, in the presence of hCG, FSH or FGF, the production of IGF-I by the coculture largely exceeded that expected from the monocultures. Moreover, stimulation of the coculture with both hCG and FSH resulted in a further increase in IGF-I production. These results indicate that Leydig as well as Sertoli cells secrete IGF-I and that the secretion of both cell types is stimulated by the corresponding gonadotropin. In addition, they indicate that Leydig-Sertoli interactions play a role in the control of IGF-I production, supporting the contention that this growth factor plays an important role in the paracrine and autocrine control of testicular functions.
Naville
D
D
INSERM U 307, Hôpital Debrousse, Lyon, France.
Chatelain
P G
PG
Avallet
O
O
Saez
J M
JM
D000818:Animals; D002450:Cell Communication; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis*; D007985:Leydig Cells / Q000378:metabolism*; D008297:Male; D012708:Sertoli Cells / Q000378:metabolism*; D013002:Somatomedins / Q000096:biosynthesis*; D013552:Swine
D006063:Chorionic Gonadotropin; D013002:Somatomedins; D005346:Fibroblast Growth Factors; D007334:Insulin-Like Growth Factor I; D005640:Follicle Stimulating Hormone