Purity determination by a combination of spectrophotometric and liquid chromatographic methods: application to 13-cis-retinoic acid.
A method is described to determine the purity of 13-cis-retinoic acid. Spectrophotometry is used to determine the non-UV-absorbing impurities, and HPLC is used to determine the UV-absorbing impurities. In combination, these methods permit determination of the purity. An authentic standard is used in developing this method, but it is not needed in the application of the method.
Gadde
R R
RR
Bristol-Myers Squibb, Pharmaceutical Research Institute, Buffalo, NY 14213.
Burton
F W
FW
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D012016:Reference Values; D013053:Spectrophotometry / Q000379:methods*; D014212:Tretinoin / Q000032:analysis* / Q000302:isolation & purification
D014212:Tretinoin