title: Molecular cloning of porcine parvovirus DNA for the purpose of obtaining viral antigen synthesis in bacteria.
abstract: An expression of porcine parvovirus 1.05 kb DNA fragment was obtained in Escherichia coli under the control both of Pr-lambda and lac-promoters. The product of expression under Pr-control was demonstrated to show PPV-specific antigenic properties. Its electrophoretic mobility corresponded to a molecular weight of approximately 45 KD.
authors.affiliation: All-Union Institute of Experimental Veterinary Medicine, Moscow, UdSSR.
authors.forename: A D
authors.initials: AD
authors.lastname: Zaberezhny
authors.forename: A L
authors.initials: AL
authors.lastname: Konorova
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis*; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D010321:Parvoviridae / Q000235:genetics*; D013552:Swine
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral