Bacterial ferritin contains 24 haem groups.
Pseudomonas aeruginosa bacterioferritin, also known as cytochrome b1 or cytochrome b557, has been isolated with 9 haems per 24 subunits. Various forms of the protein have been prepared including the completely haem-free protein and the fully haem-loaded protein with 24 haems per 24 subunits. The presence of the core does not significantly affect haem addition or removal. The absorbance ratio of the non-haem-iron-loaded protein, 278 nm:417 nm (oxidised), can be used to estimate the haem loading.
Kadir
F H
FH
Centre for Metalloprotein Spectroscopy and Biology, School of Chemical Sciences, University of East Anglia, Norwich, UK.
Moore
G R
GR
D001426:Bacterial Proteins; D003573:Cytochrome b Group / Q000032:analysis*; D005293:Ferritins / Q000032:analysis*; D006418:Heme / Q000032:analysis*; D007700:Kinetics; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D013056:Spectrophotometry, Ultraviolet
D001426:Bacterial Proteins; D003573:Cytochrome b Group; D006418:Heme; D005293:Ferritins; C018032:bacterioferritin