The effect of mafenide on dihydropteroate synthase.
Using intact bacterial cells, it was found that Pseudomonas aeruginosa was more susceptible to mafenide than Escherichia coli, that p-aminobenzoic acid (pABA) did not reverse or prevent inhibition by mafenide and that pABA itself was inhibitory. Under the experimental conditions used in these studies, pABA was more inhibitory to E. coli than to P. aeruginosa. It is proposed that pABA could be of use in the topical treatment of burn wounds. At the enzyme level, it was shown that mafenide did not inhibit dihydropteroate synthase. Thus, mafenide appeared not to exert its inhibitory effects in the same manner as the structurally related sulphonamides.
Eagon
R G
RG
Department of Microbiology, University of Georgia, Athens 30602.
McManus
A T
AT
D010129:4-Aminobenzoic Acid / Q000494:pharmacology; D004094:Dihydropteroate Synthase / Q000037:antagonists & inhibitors*; D004926:Escherichia coli / Q000201:enzymology; D008272:Mafenide / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D011189:Potassium Chloride / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000201:enzymology; D013424:Sulfanilamides / Q000494:pharmacology; D013449:Sulfonamides / Q000494:pharmacology*; D014166:Transferases / Q000037:antagonists & inhibitors*
D013424:Sulfanilamides; D013449:Sulfonamides; D008272:Mafenide; D011189:Potassium Chloride; D014166:Transferases; D004094:Dihydropteroate Synthase; D010129:4-Aminobenzoic Acid