[The effect of Cu2+, Zn2+ cations and biogenic amines on the nuclear poly(ADP-ribose) polymerase activity in the rat brain].
Study of the effects of Cu2+, Zn2+ cations and polyamines, spermine and spermidine, on the nuclear poly(ADP-ribose)polymerase activity of rat brain was carried out. It was shown that low concentrations of Cu2+ stimulate the activity of purified poly(ADP-ribose)polymerase. The poly(ADP-ribose)polymerase activity was increased 1.4-fold at 5 microM Cu2+. A further increase of Cu2+ concentration inhibited the enzymatic activity; at 50 microM Cu2+ the polymerase activity appeared to be fully inhibited. It was shown that Zn2+ inhibited only the poly(ADP-ribose)polymerase activity. Zn2+ at a concentration of 125 microM fully inhibited the enzymatic activity. Spermine and spermidine stimulated the poly(ADP-ribose)polymerase activity of brain nuclei of newborn and old rats.
Zaalishvili
T M
TM
Dzhaparidze
N Sh
NSh
Sabelashvili
D Sh
DSh
Michilashvili
R D
RD
D000375:Aging / Q000378:metabolism; D000818:Animals; D015317:Biogenic Polyamines / Q000494:pharmacology*; D001921:Brain / Q000201:enzymology*; D002413:Cations, Divalent / Q000494:pharmacology; D002467:Cell Nucleus / Q000201:enzymology; D003300:Copper / Q000494:pharmacology*; D004789:Enzyme Activation; D066298:In Vitro Techniques; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D051381:Rats; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology*
D015317:Biogenic Polyamines; D002413:Cations, Divalent; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D013096:Spermine; D003300:Copper; D011065:Poly(ADP-ribose) Polymerases; D015032:Zinc; D013095:Spermidine
Vliianie kationov Cu2+, Zn2+ i biogennykh poliaminov na iadernuiu poli(ADP-riboza) polimeraznuiu aktivnost' golovnogo mozga krys.