title: Immunohistochemical localization of pituitary gonadotrophins and gonadal steroids confirms the 'two-cell, two-gonadotrophin' hypothesis of steroidogenesis in the human ovary.
abstract: Ovaries from 37 women with normal menstrual cycles were analysed for localization of pituitary gonadotrophins and gonadal steroids using an immunohistochemical method. In the follicular phase, FSH and oestradiol-17 beta localized in the granulosa layer, and LH, progesterone and testosterone localized in the internal thecal layer. In the luteal phase, gonadotrophins and steroids localized in luteal cells. Particularly in the early luteal phase, FSH and oestradiol-17 beta localized in large luteal cells, and LH, progesterone and testosterone localized in small luteal cells. The results of the present immunohistochemical analysis confirm the two-cell, two-gonadotrophin hypothesis of steroidogenesis in the human ovary.
authors.affiliation: Department of Obstetrics and Gynaecology, Wakayama Medical College, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kobayashi
authors.forename: R
authors.initials: R
authors.lastname: Nakano
authors.forename: A
authors.initials: A
authors.lastname: Ooshima
mesh_terms: D000328:Adult; D000818:Animals; D003338:Corpus Luteum / Q000737:chemistry / Q000166:cytology; D004958:Estradiol / Q000032:analysis; D004971:Estrus / Q000378:metabolism*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000032:analysis; D012739:Gonadal Steroid Hormones / Q000032:analysis*; D006065:Gonadotropins, Pituitary / Q000032:analysis*; D006107:Granulosa Cells / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D007986:Luteinizing Hormone / Q000032:analysis; D010053:Ovary / Q000737:chemistry*; D011374:Progesterone / Q000032:analysis; D013739:Testosterone / Q000032:analysis; D013799:Theca Cells / Q000737:chemistry
chemical_list: D012739:Gonadal Steroid Hormones; D006065:Gonadotropins, Pituitary; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone