title: Biological activity of bacterial surface components: bacterial extracts and defined bacterial cell wall components as immunomodulators.
abstract: Bacterial extracts obtained from pathogenic strains occurring in lung infections (Broncho Vaxom) or urogenital infections (Urovaxom) as well as defined surface components of Gram-negative bacteria purified from bacteria or obtained by chemical synthesis were tested for their immunomodulatory properties in a murine system. The bacterial extracts were able to act as immunogens inducing an antigen-specific response. Both the bacterial extracts and the purified bacterial cell wall components constituted polyclonal activators of murine splenic B cells, as demonstrated by proliferation assays measuring the incorporation of [3H]thymidine into DNA. They were also able to act as immunoadjuvants increasing the SRBC and the BSA-TNP specific immune response, and could induce tumor cytotoxicity in bone marrow-derived macrophages. The results show that bacterial extracts and defined bacterial surface components constitute immunogens as well as immunomodulators in vitro and in vivo.
authors.affiliation: Institut für Immunbiologie der Universität, Freiburg, Federal Republic of Germany.
authors.forename: W G
authors.initials: WG
authors.lastname: Bessler
authors.forename: B
authors.initials: B
authors.lastname: Kleine
authors.forename: C
authors.initials: C
authors.lastname: Martinez Alonso
authors.forename: L
authors.initials: L
authors.lastname: Biesert
authors.forename: M
authors.initials: M
authors.lastname: Strecker
authors.forename: K H
authors.initials: KH
authors.lastname: Wiesmüller
authors.forename: J
authors.initials: J
authors.lastname: Metzger
authors.forename: G
authors.initials: G
authors.lastname: Jung
mesh_terms: D000818:Animals; D051379:Mice; D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000942:Antigens, Bacterial; D001402:B-Lymphocytes / Q000276:immunology; D001419:Bacteria; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D006090:Gram-Negative Bacteria / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D008262:Macrophage Activation / Q000276:immunology; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000276:immunology; D000095983:Bacterial Lysates
chemical_list: D000276:Adjuvants, Immunologic; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D010455:Peptides; C030259:Broncho-Vaxom; D000095983:Bacterial Lysates; C050501:OM 89