Altered transcriptional activity of c-fos promoter plasmids in v-raf-transformed NIH 3T3 cells.
In cells transformed by v-raf, an oncogenic counterpart of the serine/threonine kinase Raf-1, regulatory elements of the c-fos promoter were active under conditions of cell growth or stimulation for which they were inactive in untransformed control cells. This suggests that v-raf transforms by deregulating transcription of early response genes.
Siegfried
Z
Z
Howard Hughes Medical Institute, Department of Biochemistry, New York University Medical Center, New York 10016.
Ziff
E B
EB
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D005914:Globins / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D027384:Oncogene Proteins v-raf; D009857:Oncogenes; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016760:Proto-Oncogene Proteins c-fos; D011519:Proto-Oncogenes; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection
D015345:Oligonucleotide Probes; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D015682:Retroviridae Proteins, Oncogenic; D005914:Globins; D011505:Protein-Tyrosine Kinases; D027384:Oncogene Proteins v-raf