[Determination of ranunculin in Pulsatilla chinensis and synthetic ranunculin by reversed phase HPLC].
A method for the determination of ranunculin in Pulsatilla chinensis and synthetic ranunculin by reversed phase HPLC using betamethasone as internal standard is described. The column employed was a 10 micron LiChrosorb RP-18 (230 x 14 mm), the eluting solvent consisted of methanol-water (40:10, V/V) and the effluent was monitored at 225 nm. The advantages of this method are simple, rapid and accurate. The coefficients of variation are less than 1%.
Zhang
X Q
XQ
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Liu
A R
AR
Xu
L X
LX
D000890:Anti-Infective Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005663:Furans / Q000032:analysis* / Q000302:isolation & purification; D008759:Methylglycosides / Q000032:analysis* / Q000302:isolation & purification
D000890:Anti-Infective Agents; D004365:Drugs, Chinese Herbal; D005663:Furans; D008759:Methylglycosides; C039859:ranunculin