High level synthesis of immunoglobulins in Chinese hamster ovary cells.
The expression of lambda L and microH chain cDNA was examined in Chinese hamster ovary cells. Each cDNA was linked to a different, amplifiable, selectable drug marker gene, and expression was monitored in the presence of increasing concentrations of the selective drugs. Cells were obtained that produced greater than 60 micrograms/10(6) cells/48 h of assembled antibody. This Chinese hamster ovary cell-synthesized IgM was polymeric, and exhibited specific hapten binding and C fixation. The expression strategy employed here may prove useful for the future production of genetically engineered antibodies and other multi-subunit proteins.
Wood
C R
CR
Genetics Institute, Inc., Cambridge, MA 02140.
Dorner
A J
AJ
Morris
G E
GE
Alderman
E M
EM
Wilson
D
D
O'Hara
R M
RM
Kaufman
R J
RJ
D000818:Animals; D000906:Antibodies / Q000235:genetics*; D000918:Antibody Specificity; D001483:Base Sequence; D002352:Carrier Proteins / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D003168:Complement Fixation Tests; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics; D000091342:Endoplasmic Reticulum Chaperone BiP; D005818:Genetic Engineering / Q000379:methods; D006360:Heat-Shock Proteins; D007146:Immunoglobulin lambda-Chains / Q000235:genetics; D007148:Immunoglobulin mu-Chains / Q000235:genetics; D007136:Immunoglobulins / Q000235:genetics*; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D009596:Nitrophenols / Q000276:immunology; D010648:Phenylacetates; D011994:Recombinant Proteins; D014162:Transfection
D000906:Antibodies; D002352:Carrier Proteins; D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; D007146:Immunoglobulin lambda-Chains; D007148:Immunoglobulin mu-Chains; D007136:Immunoglobulins; D018832:Molecular Chaperones; D009596:Nitrophenols; D010648:Phenylacetates; D011994:Recombinant Proteins; C026242:4-hydroxy-5-nitrophenyl acetic acid; D004247:DNA