title: [Phenotypic and functional characterization of cloned T-lymphocytes from cerebrospinal fluid of patients with multiple sclerosis].
abstract: T cell clones derived from the cerebrospinal fluid of patients with multiple sclerosis were investigated for their ability to produce IL2, IL4, IFN gamma and TNF alpha. As controls, liver infiltrating T lymphocyte clones from patients with chronic active hepatitis were used. All CSF clones (both CD4+ and CD8+) produced high amounts of IFN gamma and particularly of TNF alpha. TNF was synthesized in a significantly higher amount than control clones. Moreover, they were capable of secreting IL2 but not IL4. From our results we conclude that CSF-CD4+ T clones could constitute a subset with functional properties similar to those of the Th1/inflammatory cells of the mouse. The unusually high amount of TNF produced by CSF derived T cell clones strongly suggests a significant role for this cytokine in MS immunopathogenesis.
authors.affiliation: Fondazione Andrea Cesalpino, I Clinica Medica, Università La Sapienza, Roma.
authors.forename: V
authors.initials: V
authors.lastname: Barnaba
authors.forename: R
authors.initials: R
authors.lastname: Benvenuto
authors.forename: C
authors.initials: C
authors.lastname: Buttinelli
authors.forename: M
authors.initials: M
authors.lastname: Salvetti
authors.forename: L
authors.initials: L
authors.lastname: Toma
authors.forename: P
authors.initials: P
authors.lastname: Fiori
mesh_terms: D000328:Adult; D002455:Cell Division; D002555:Cerebrospinal Fluid / Q000166:cytology; D002999:Clone Cells; D016207:Cytokines / Q000378:metabolism; D003601:Cytotoxicity Tests, Immunologic; D006801:Humans; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid*; D010641:Phenotype; D013601:T-Lymphocytes / Q000502:physiology*
chemical_list: D016207:Cytokines
vernacular_title: Caratterizzazione fenotipica e funzionale di cloni T linfocitari di derivazione liquorale in pazienti affetti da sclerosi multipla.