A physical map and analysis of the murine C kappa-RS region show the presence of a conserved element.
Lambda-producing B lymphocytes have frequently deleted one or, more often, both Ig kappa loci. This deletion is mediated by the rearrangement of an element which lies 3' of C kappa and which is called RS (recombining sequence) in the mouse and Kde (kappa-deleting element) in the human. The tight correlation between V lambda to J lambda rearrangements and an RS-mediated deletion may indicate that sequences in the C kappa-RS region are controlling the activation of the Ig lambda locus. We have linked the C kappa exon and the RS element by phage cloning and compared the C kappa-RS region to the previously cloned human C kappa-Kde region. The distance between C kappa and RS is 25 kb and is thus similar to the distance of 24 kb separating the human C kappa exon and Kde element. Both mouse and man carry a conserved sequence of 470 bp (Rx) which lies 9 kb 3' of the mouse C kappa and 12 kb 3' of the human C kappa exon. The conserved mouse Rx sequence contains part of the kappa 3' enhancer.
Müller
B
B
Institute for Genetics, University of Cologne.
Stappert
H
H
Reth
M
M
D000818:Animals; D001483:Base Sequence; D015328:Gene Rearrangement, B-Lymphocyte, Light Chain / Q000276:immunology*; D007127:Immunoglobulin Constant Regions / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011995:Recombination, Genetic / Q000235:genetics; D015183:Restriction Mapping
D007127:Immunoglobulin Constant Regions; D007145:Immunoglobulin kappa-Chains