title: Tissue-plasminogen activator stimulates endothelial cell migration in wound assays.
abstract: The ability of tissue plasminogen activator (tPA) to induce human umbilical vein endothelial (HUVE) cell migration was studied using an in vitro, serum-free wound assay system. At pharmacological doses, tPA stimulated HUVE cell migration dose-dependently. Treatment of cells with epsilon amino caproic acid (EACA) to detach cell-surface and extracellular matrix bound plasminogen, which could lead to plasmin generation, resulted in increased HUVEcell migration on stimulation with tPA.Plasminogen activator inhibitor-1 (PAI-1), a natural plasminogen activator inhibitor, abolished tPA-induced HUVEcell migration. These results demonstrate for the first time that tPA is capable of stimulating endothelial cell migration in wound assays and this effect is susceptible to PAI-1 inhibition.
authors.affiliation: INSERM U150, Hopital Lariboisiere, Paris, France.
authors.forename: A L
authors.initials: AL
authors.lastname: Inyang
authors.forename: G
authors.initials: G
authors.lastname: Tobelem
mesh_terms: D002465:Cell Movement / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D015849:Plasminogen Inactivators / Q000494:pharmacology; D010959:Tissue Plasminogen Activator / Q000494:pharmacology*; D014947:Wounds and Injuries / Q000503:physiopathology*
chemical_list: D015849:Plasminogen Inactivators; D010959:Tissue Plasminogen Activator