Diminished quality of two-dimensional (2-D) gel electrophoresis after freezing of the first-dimension gels.
Two-dimensional (2-D) gels were compared before and after freezing mini isoelectric focusing (IEF) gels in their glass tubes at -20 degrees C. Overnight storage of mini IEF gels significantly reduced the separation, resolution and number of proteins in 2-D gels.
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Neuroendocrine Research Laboratory, Veterans Affairs Medical Center, North Chicago, Illinois 60064.
D000818:Animals; D001923:Brain Chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D005615:Freezing; D005898:Glass; D007525:Isoelectric Focusing; D009419:Nerve Tissue Proteins / Q000737:chemistry; D051381:Rats
D009419:Nerve Tissue Proteins