Cloning of the citrate permease gene of Lactococcus lactis subsp. lactis biovar diacetylactis and expression in Escherichia coli.
The citrate plasmid (Cit+ plasmid) from Lactococcus lactis subsp. lactis biovar diacetylactis was cloned into the EcoRI site of plasmid pUC18. This recombinant plasmid enabled Escherichia coli K-12 to transport and utilize citrate as a source of energy, indicating expression of the citrate permease from L. lactis biovar diacetylactis. The citrate permease was under the control of the lac promoter of pUC18. Genetic expression of the Cit+ plasmid in maxicells revealed that the plasmid encoded two polypeptides of 47 and 32 kilodaltons, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Sesma
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Centro de Referencia para Lactobacilos, Tucumán, Republic of Argentina.
Gardiol
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de Ruiz Holgado
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AP
de Mendoza
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D
D001426:Bacterial Proteins; D001693:Biological Transport, Active; D002951:Citrates / Q000378:metabolism; D019343:Citric Acid; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005798:Genes, Bacterial; D013294:Lactococcus lactis / Q000201:enzymology* / Q000235:genetics; D026901:Membrane Transport Proteins / Q000235:genetics*; D027361:Organic Anion Transporters; D010957:Plasmids; D015183:Restriction Mapping
D001426:Bacterial Proteins; D002951:Citrates; D026901:Membrane Transport Proteins; D027361:Organic Anion Transporters; D019343:Citric Acid; C065431:citP protein, Lactococcus lactis