title: Differential effects of fat deficiency on hepatic and pulmonary drug metabolizing enzymes in rat and mouse.
abstract: In the present study, we have evaluated the effect of dietary fat deprivation on the carcinogen/drug metabolizing enzymes in rats and mice. In rats, hepatic AHH, Cyt.P-450 Cyt.b5 and Cyt.c-reductase were significantly decreased due to fat deficiency, whereas in lungs, AHH and Cyt.c-reductase were decreased without any change in Cyt.P-450 level. In mice, feeding of fat-free diet did not cause any alteration in hepatic AHH and Cyt.P-450, but the levels of Cyt.b5 and Cyt.c-reductase were significantly reduced. In contrast to liver, Cyt.P-450 and Cyt.b5 were increased in lungs. Activity of UDPGT was lowered both in liver and lungs of rats whereas GST and GSH were increased in liver only. In mice, a decrease in UDPGT and appreciable increases in GST and GSH in liver were observed. However, in lungs, UDPGT activity was enhanced by feeding fat-free diet. These observations suggest that mice and rats respond differentially to the depletion of fat in the diet.
authors.affiliation: Department of Biophysics, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: I B
authors.initials: IB
authors.lastname: Koul
authors.forename: A
authors.initials: A
authors.lastname: Koul
authors.forename: R K
authors.initials: RK
authors.lastname: Gandhi
authors.forename: R R
authors.initials: RR
authors.lastname: Sharma
authors.forename: K L
authors.initials: KL
authors.lastname: Khanduja
mesh_terms: D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D015786:Cytochromes b5 / Q000378:metabolism; D004041:Dietary Fats / Q000008:administration & dosage*; D014453:Glucuronosyltransferase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008168:Lung / Q000201:enzymology*; D008297:Male; D051379:Mice; D009245:NADH Dehydrogenase / Q000378:metabolism; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004041:Dietary Fats; D004364:Pharmaceutical Preparations; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; D001189:Aryl Hydrocarbon Hydroxylases; D009245:NADH Dehydrogenase; D014453:Glucuronosyltransferase; D005982:Glutathione Transferase; D005978:Glutathione