title: Modification by some antagonists of the shape changes of venous endothelial cells in response to inflammatory agents in vitro.
abstract: Endothelial cells lining guinea pig inferior venae cavae change shape when exposed to histamine, bradykinin, A23187 or platelet-activating factor (PAF) in vitro. Pre-treatment of the endothelium with isoprenaline or quin 2 significantly reduced the shape changes produced in response to histamine, bradykinin and A23187, but not those to PAF. Since both isoprenaline and quin 2 may reduce the concentration of cytoplasmic Ca++, the former by raising cyclic AMP (cAMP) levels and the latter by acting as a Ca++ buffer, the results provide further evidence for the involvement of Ca++ in the responses of large vein endothelial cells to inflammatory agents in vitro. The effects of pre-treating the endothelium with the histamine receptor-blockers mepyramine (H1) or cimetidine (H2), or the bradykinin receptor-blockers des-arg9[leu8] bradykinin (B1) or des-arg[Hyp3, Thi5,8,D-Phe7] bradykinin (B2) suggest that the response to histamine is both H1 and H2 receptor-mediated, while the response to bradykinin is only B2 receptor-mediated.
authors.affiliation: Department of Pharmacology, School of Pharmacy, Leicester Polytechnic, UK.
authors.forename: A M
authors.initials: AM
authors.lastname: Northover
mesh_terms: D000634:Aminoquinolines / Q000494:pharmacology; D000818:Animals; D001920:Bradykinin / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002927:Cimetidine / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology*; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology*; D006633:Histamine Antagonists / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000494:pharmacology*; D011738:Pyrilamine / Q000494:pharmacology; D014682:Vena Cava, Inferior
chemical_list: D000634:Aminoquinolines; D006633:Histamine Antagonists; D010972:Platelet Activating Factor; C058532:HOE k86-4321; D000001:Calcimycin; C051090:bradykinin, Leu(8)-des-Arg(9)-; D002927:Cimetidine; D006632:Histamine; D011738:Pyrilamine; D007545:Isoproterenol; C035739:Quin2; D001920:Bradykinin; D002118:Calcium