Criteria to indicate testosterone administration.
A detection method for testosterone administration was developed using radioimmunoassay to measure the urinary ratios of testosterone (T) to epitestosterone (E) and to luteinizing hormone (LH). A comparative study of the effect on these ratios of a single intramuscular injection of testosterone heptanoate followed by stimulation with human chorionic gonadotrophin (HCG) in three normal men was undertaken. To allow immediate investigation, a commercially supplied epitestosterone antiserum was used. This study showed that both T/E and T/LH ratios could be used to detect testosterone administration, the latter also being an indicator of HCG use due to cross-reactivity with the LH antiserum. Subsequently, an epitestosterone antiserum of superior specificity was raised and used in a study to demonstrate the insignificant effect of exercise on these ratios. Finally, an intramuscular injection of a combined preparation of testosterone/epitestosterone heptanoates resulted in raised ratios of T/LH but not of T/E. This demonstrated the importance of the T/LH ratio in circumstances where the T/E ratio can be easily circumvented.
Kicman
A T
AT
Drug Control and Teaching Centre, King's College, London University.
Brooks
R V
RV
Collyer
S C
SC
Cowan
D A
DA
Nanjee
M N
MN
Southan
G J
GJ
Wheeler
M J
MJ
D000328:Adult; D004300:Doping in Sports; D004845:Epitestosterone / Q000097:blood / Q000652:urine; D006801:Humans; D007273:Injections, Intramuscular; D007986:Luteinizing Hormone / Q000097:blood / Q000652:urine; D008297:Male; D011863:Radioimmunoassay; D015813:Substance Abuse Detection; D013739:Testosterone / Q000008:administration & dosage* / Q000097:blood / Q000652:urine
D013739:Testosterone; D004845:Epitestosterone; D007986:Luteinizing Hormone