title: Rapid kinetics of an N-terminal mutant of cyanobacterial ribulose-1,5-bisphosphate carboxylase/oxygenase.
abstract: The transient changes in absorption of visible light upon addition of ribulose 1,5-bisphosphate to Co2(+)-activated ribulose-1,5-bisphosphate carboxylase/oxygenase were used to show altered catalytic properties of a mutant form of the enzyme from Anacystis nidulans. The mutant form of the enzyme had a modified N-terminus and a 10-fold greater Km for ribulose 1,5-bisphosphate than the natural cyanobacterial enzyme.
authors.affiliation: Department of Biochemistry and Biophysics, University of Göteborg, Sweden.
authors.forename: R
authors.initials: R
authors.lastname: Brändén
authors.forename: A J
authors.initials: AJ
authors.lastname: Keys
authors.forename: M A
authors.initials: MA
authors.lastname: Parry
mesh_terms: D002384:Catalysis; D003035:Cobalt / Q000494:pharmacology; D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics; D007700:Kinetics; D009154:Mutation; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics / Q000378:metabolism*; D012274:Ribulosephosphates / Q000378:metabolism
chemical_list: D012274:Ribulosephosphates; C001933:ribulose-1,5 diphosphate; D003035:Cobalt; D012273:Ribulose-Bisphosphate Carboxylase