title: Isolation of a second cryptic plasmid from Mycoplasma mycoides subsp. mycoides.
abstract: Plasmids have rarely been detected in organisms constituting the genus Mycoplasma. Recently, the isolation of a cryptic plasmid from Mycoplasma mycoides subsp. mycoides has been described, and we report here the isolation of a second cryptic plasmid from this species. Restriction map and Southern blot analyses show that the second plasmid is distinct from the previously described plasmid, although a limited region of homology was detected. The availability of mycoplasmal cryptic plasmids may lead to the development of cloning vectors that replicate in these organisms.
authors.affiliation: Department of Comparative Medicine, University of Alabama, Birmingham 35294.
authors.forename: K
authors.initials: K
authors.lastname: Dybvig
authors.forename: M
authors.initials: M
authors.lastname: Khaled
mesh_terms: D005822:Genetic Vectors; D009176:Mycoplasma mycoides / Q000235:genetics*; D010957:Plasmids; D015183:Restriction Mapping