title: Immunoglobulin light chain transcripts with altered V regions in Burkitt's lymphoma cell lines producing short mu chains.
abstract: The Burkitt lymphoma cell lines Ly91, Ly47 and Ly66 produce short mu chains in the absence of associated light (L) chains. mu mRNA lack two-thirds of the variable (V) region due to an alternate splicing event that takes place within V genes. L chain genes were found to be rearranged in these lines and to lead to transcripts with altered V regions. Although these abnormal transcripts displayed specific abnormalities in each cell line, they shared some features, including large deletions involving the V-J junction. In one cell line, L chain transcripts made up of either V kappa I subgroup gene J kappa 3 or V kappa IV gene-J kappa 4 recombination products bore a similar alteration: the donor splice site of the rearranged J kappa segment was modified, leading to the alternate use of the donor site of either the leader peptide exon or the next 3' unrearranged J kappa segment.
authors.affiliation: CNRS URA 1172, Faculté des Sciences, Poitiers, France.
authors.forename: M
authors.initials: M
authors.lastname: Cogné
authors.forename: S
authors.initials: S
authors.lastname: Mounir
authors.forename: P
authors.initials: P
authors.lastname: Aucouturier
authors.forename: J L
authors.initials: JL
authors.lastname: Preud'homme
authors.forename: F
authors.initials: F
authors.lastname: Nau
authors.forename: P
authors.initials: P
authors.lastname: Guglielmi
mesh_terms: D001483:Base Sequence; D002051:Burkitt Lymphoma / Q000276:immunology*; D002872:Chromosome Deletion; D004247:DNA / Q000032:analysis; D005091:Exons; D015321:Gene Rearrangement; D006801:Humans; D007133:Immunoglobulin Joining Region / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007148:Immunoglobulin mu-Chains / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis*; D014407:Tumor Cells, Cultured
chemical_list: D007133:Immunoglobulin Joining Region; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D007148:Immunoglobulin mu-Chains; D012333:RNA, Messenger; D004247:DNA