[Immunocytochemical evaluation of estrogen receptors in histological specimens of primary breast cancer].
Immunocytochemical methods (ER-ICA) by using monoclonal antibodies were applied to determine the presence of estrogen receptors (ER) in 44 primary breast cancers in women. Of this 48% of the tumours were classified as receptor positive. In these tumours the nuclei had a clearly positive heterogenous colouration. In three cases a positive reaction was also found in benign epithelial cells of the breast. ER determinations by ER-ICA method were compared with quantitative analysis carried out by using radioligand and immunoenzymatic methods. There was a strong correlation between immunocytochemical ER evaluations and quantitative methods. We also found a correlation of the menopause state and patients age and ER content.
Gorczyca
W
W
Zakład Patomorfologii Nowotworów Instytutu Patologii PAM.
Olszewski
W
W
Woyke
S
S
Chrapusta
S
S
Konopka
B
B
Paszko
Z
Z
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D004967:Estrogens / Q000737:chemistry / Q000378:metabolism*; D005188:False Negative Reactions; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008875:Middle Aged; D011869:Radioligand Assay; D011960:Receptors, Estrogen / Q000032:analysis / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D004967:Estrogens; D011960:Receptors, Estrogen
Immunocytochemiczna ocena receptora estrogenów w preparatach histologicznych pierwotnych raków sutka.