title: Immunolocalization of secretory protein-I or chromogranin A in amphibian urinary bladder granular cell granules.
abstract: The presence of secretory protein-I (SP-I) or chromogranin A (CGA) in granules isolated from the granular cells of the amphibian urinary bladder epithelium was investigated using ultraimmunohistochemistry. Granules were isolated by cell fractionation using Percoll density gradients. SP-I was isolated and purified from bovine parathyroid glands. Antibodies were raised in rabbits and purified by affinity chromatography. Ultraimmunocytochemistry, employing the avidin-biotin-peroxidase (ABC-complex) procedure, was used to localize SP-I on thin sections of isolated granules. About 27% of the granules from control (-ADH) cells were SP-I+, while 51% of the granules fractionated from hormone treated (+ADH) cells were positive for this protein (p less than 0.0001). Accordingly, granules from ADH-treated cells also showed a significant (p less than 0.0001) increase in total protein.
authors.affiliation: Department of Anatomy, Baylor College of Dentistry, Dallas, TX 75246.
authors.forename: W L
authors.initials: WL
authors.lastname: Davis
authors.forename: K O
authors.initials: KO
authors.lastname: Schmid
authors.forename: J W
authors.initials: JW
authors.lastname: Huettner
authors.forename: G R
authors.initials: GR
authors.lastname: Farmer
authors.forename: B H
authors.initials: BH
authors.lastname: Jacoby
authors.forename: D B
authors.initials: DB
authors.lastname: Goodman
mesh_terms: D000818:Animals; D002024:Bufo marinus / Q000378:metabolism*; D053379:Chromogranin A; D002864:Chromogranins / Q000378:metabolism*; D003594:Cytoplasmic Granules / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D001743:Urinary Bladder / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D053379:Chromogranin A; D002864:Chromogranins; D009419:Nerve Tissue Proteins