[The alternation of CD8+ cells and its subpopulations by serial monitoring of peripheral blood lymphocytes of renal allograft recipients].
To catch and treat the initial alternation of a rejection and infection, we have introduced the serial analysis of lymphocyte surface antigens of peripheral blood lymphocytes by flow cytometry for 9 cases of renal allo-graft recipients since September 30 in 1988. In three recipients without rejection and infection, we found that T8+(CD8+), T8+Mo1+(CD11b+), T8+Mo1-(CD11b-), and T8+IL-2R+(CD25+) subsets were variable for first 20 days and then they were stable. However, another activated CD8+ T-cell subset such as T8+I2+(HLA-DR+) subset gradually increased after first 20 days, so that we investigated the different processes between these two activated subsets. On primary rejection of 5 cases, T8+I2+ and T8+IL-2R+ subsets showed peak formations within 2 days before the rejection. Two of these 5 cases resisted to a primary rejection therapy and showed rebounding arise of these subsets. We could easily convert to OKT-3 rescue therapy and treat them successfully. In order to catch the initial alternation of the primary rejection and treat the rebounding reaction successfully, we should monitor the T8+I2+ and T8+IL-2R+ subsets daily for first 2 weeks and after then 3 times a week.
Mori
Y
Y
Department of Surgery, Saga Prefectural Hospital.
Shiramizu
T
T
Yayama
T
T
Oka
N
N
D000328:Adult; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D016827:CD8 Antigens; D005260:Female; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D008991:Monitoring, Physiologic; D013601:T-Lymphocytes / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens