Inducible production of macrophage colony-stimulating factor (CSF-1) by malignant and normal human T cells.
The CEM-ON malignant T cell line and long-term cultured normal T cells can be induced to release CSF-1 in their culture supernatants. Chemical inducers (PMA + A23187) and, more interestingly, cytokines (tumor necrosis factor alpha (TNF alpha) and interleukin-1 alpha (IL-1 alpha)), as well as physiological (antigen + IL-2) or specific (anti-CD3 + IL-2 or PMA) stimuli, lead to rapid transient colony-stimulating factor-1 (CSF-1) gene expression and production of biologically active CSF-1. These data suggest that CSF-1 may play a role in the early phases of immune response.
Praloran
V
V
Laboratoire d'Hématologie, Centre Hospitalier Régional, Nantes, France.
Gascan
H
H
Papin
S
S
Chevalier
S
S
Trossaërt
M
M
Boursier
M C
MC
D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D015152:Blotting, Northern; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D015458:Leukemia, T-Cell / Q000276:immunology / Q000378:metabolism*; D016173:Macrophage Colony-Stimulating Factor; D009000:Monocytes / Q000166:cytology; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D000945:Antigens, Differentiation, T-Lymphocyte; D003115:Colony-Stimulating Factors; D007375:Interleukin-1; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D000001:Calcimycin; D016173:Macrophage Colony-Stimulating Factor; D013755:Tetradecanoylphorbol Acetate