title: In vitro antibacterial activity of cefotaxime and desacetylcefotaxime, alone or in combination, against gram-positive cocci.
abstract: The in vitro activity of cefotaxime and desacetylcefotaxime against Staphylococcus aureus, Staph. epidermidis and Streptococcus pyogenes was investigated. Synergy studies were performed using time-kill curves and the chequerboard test. The time-kill curves were performed on five strains each of Staph. aureus, Staph. epidermidis and Strep. pyogenes; cefotaxime and desacetylcefotaxime were tested alone or in combination at MIC and sub-MIC values. The chequerboard test was performed in microtitre plates on ten strains each of Staph. aureus, Staph. epidermidis and Strep. pyogenes: the results were interpreted by the fractional inhibitory concentration index. In some cases both methods showed synergistic interaction against the staphylococci tested. Indifference was observed against Strep. pyogenes.
authors.affiliation: Institute of Infectious Diseases, University of Verona, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Cruciani
authors.forename: A
authors.initials: A
authors.lastname: Navarra
authors.forename: G
authors.initials: G
authors.lastname: Di Perri
authors.forename: M C
authors.initials: MC
authors.lastname: Danzi
authors.forename: E
authors.initials: E
authors.lastname: Concia
authors.forename: D
authors.initials: D
authors.lastname: Bassetti
mesh_terms: D002439:Cefotaxime / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002442:Ceftazidime / Q000494:pharmacology; D002443:Ceftriaxone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004359:Drug Therapy, Combination; D008826:Microbial Sensitivity Tests; D013211:Staphylococcus aureus / Q000187:drug effects*; D013212:Staphylococcus epidermidis / Q000187:drug effects*; D013297:Streptococcus pyogenes / Q000187:drug effects*
chemical_list: C029281:desacetylcefotaxime; D002443:Ceftriaxone; D002442:Ceftazidime; D002439:Cefotaxime