Identification of a second inducible DNA-protein interaction in the kappa immunoglobulin enhancer.
Control of kappa immunoglobulin light-chain gene expression requires the interaction of tissue specific and developmentally regulated DNA-binding proteins with the kappa gene enhancer. Deletion of enhancer sequences upstream from the NF-kB binding site has been shown to impair enhancer function, implying additional proteins may interact with these sequences. In surveying this region for sites of protein binding, a novel DNA-protein interaction, designated kBF-A, was detected. The binding activity of this factor appears to be specific to activated pre-B cells and correlates with high level induction of kappa transcription in these cells.
Nelms
K
K
Institute of Human Genetics, University of Minnesota, Minneapolis 55455.
Hromas
R
R
Van Ness
B
B
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D015342:DNA Probes; D004268:DNA-Binding Proteins / Q000378:metabolism*; D003850:Deoxyribonuclease I; D004742:Enhancer Elements, Genetic; D005803:Genes, Immunoglobulin; D006801:Humans; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010957:Plasmids; D011817:Rabbits; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D015342:DNA Probes; D004268:DNA-Binding Proteins; D007145:Immunoglobulin kappa-Chains; D015345:Oligonucleotide Probes; D003850:Deoxyribonuclease I