title: Occurrence of hydrin 2 (vasotocinyl-Gly), a new hydroosmotic neurohypophyseal peptide, in secretory granules isolated from the frog (Rana esculenta) neurointermediate pituitary.
abstract: Neurohypophyseal secretory granules have been purified from the frog (Rana esculenta) neurointermediate pituitary gland by sucrose gradient centrifugation, and their polypeptide content has been analyzed by reverse-phase high-pressure liquid chromatography. Aside from vasotocin, mesotocin, and their associated neurophysins, hydrin 2 (vasotocinyl-Gly), previously identified in hydrochloric acid extracts, has been recognized. This finding supports the previous suggestion that hydrin 2, a peptide active on the water permeability of frog bladder and frog skin, is a secreted hormone involved in osmoregulation specific to amphibians. Hydrin 2 has not been found in neurosecretory granules of birds such as the goose.
authors.affiliation: Laboratory of Biological Chemistry, University of Paris VI, France.
authors.forename: J
authors.initials: J
authors.lastname: Chauvet
authors.forename: Y
authors.initials: Y
authors.lastname: Rouillé
authors.forename: M T
authors.initials: MT
authors.lastname: Chauvet
authors.forename: R
authors.initials: R
authors.lastname: Acher
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003594:Cytoplasmic Granules / Q000032:analysis*; D010904:Pituitary Gland, Posterior / Q000032:analysis*; D011893:Rana esculenta; D014668:Vasotocin / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: C060258:hydrin 2; D014668:Vasotocin