title: Criteria to indicate testosterone administration.
abstract: A detection method for testosterone administration was developed using radioimmunoassay to measure the urinary ratios of testosterone (T) to epitestosterone (E) and to luteinizing hormone (LH). A comparative study of the effect on these ratios of a single intramuscular injection of testosterone heptanoate followed by stimulation with human chorionic gonadotrophin (HCG) in three normal men was undertaken. To allow immediate investigation, a commercially supplied epitestosterone antiserum was used. This study showed that both T/E and T/LH ratios could be used to detect testosterone administration, the latter also being an indicator of HCG use due to cross-reactivity with the LH antiserum. Subsequently, an epitestosterone antiserum of superior specificity was raised and used in a study to demonstrate the insignificant effect of exercise on these ratios. Finally, an intramuscular injection of a combined preparation of testosterone/epitestosterone heptanoates resulted in raised ratios of T/LH but not of T/E. This demonstrated the importance of the T/LH ratio in circumstances where the T/E ratio can be easily circumvented.
authors.affiliation: Drug Control and Teaching Centre, King's College, London University.
authors.forename: A T
authors.initials: AT
authors.lastname: Kicman
authors.forename: R V
authors.initials: RV
authors.lastname: Brooks
authors.forename: S C
authors.initials: SC
authors.lastname: Collyer
authors.forename: D A
authors.initials: DA
authors.lastname: Cowan
authors.forename: M N
authors.initials: MN
authors.lastname: Nanjee
authors.forename: G J
authors.initials: GJ
authors.lastname: Southan
authors.forename: M J
authors.initials: MJ
authors.lastname: Wheeler
mesh_terms: D000328:Adult; D004300:Doping in Sports; D004845:Epitestosterone / Q000097:blood / Q000652:urine; D006801:Humans; D007273:Injections, Intramuscular; D007986:Luteinizing Hormone / Q000097:blood / Q000652:urine; D008297:Male; D011863:Radioimmunoassay; D015813:Substance Abuse Detection; D013739:Testosterone / Q000008:administration & dosage* / Q000097:blood / Q000652:urine
chemical_list: D013739:Testosterone; D004845:Epitestosterone; D007986:Luteinizing Hormone