[Detection of a soluble serum antigen by a new monoclonal antibody ED8 in patients with breast cancer].
A murine hybridoma, secreting monoclonal antibody ED8, was generated by immunization with the breast cancer cell line H466B. ED8 reacts with a 300 kDa antigen present on H466B cell surface. The antigen can be detected in serum from breast cancer patients, using ELISA and immunoblotting.
Mortada
M
M
Division d'Immunocytologie appliquée, Université de Compiègne, Centre Benjamin Franklin.
Pancino
G
G
Charpin
C
C
Schlageter
M H
MH
Calvo
F
F
Roseto
A
A
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D001943:Breast Neoplasms / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology / Q000378:metabolism; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D012995:Solubility
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface
Détection par un nouvel anticorps monoclonal ED8 d'un antigène soluble dans le sérum de malades atteintes de cancer du sein.