Novel non-tumorigenic cell variants showing potentially different susceptibility to v-src-induced metastasis.
Two non-tumorigenic variant cells were isolated from UV-irradiated Balb/c 3T3 cells on the basis of their different responsiveness in phorbol ester-induced morphological change (rounding formation). They showed marked differences of lung metastatic potentials after intravenous injections of their v-src transformants into nude mice; phorbol ester-resistant variant TR4 cells transformed by v-src were hypermetastatic, whereas v-src transformants of phorbol ester-sensitive variant TR5 cells were not metastatic at all. These different metastatic responses were not observed in v-K-ras-induced transformants of the variants. These non-tumorigenic variant cells may pre-acquire the genetic alteration of certain src-specific and metastasis-associated factors. This system may be useful for genetic analysis of the induction of metastasis.
Enomoto
T
T
Department of Oncogene Research, Osaka University.
Ayaki
H
H
Nakamori
S
S
Enomoto
Y
Y
Inoue
H
H
Kakunaga
T
T
D000818:Animals; D002472:Cell Transformation, Viral / Q000235:genetics*; D004351:Drug Resistance / Q000235:genetics; D005260:Female; D011905:Genes, ras / Q000235:genetics*; D008175:Lung Neoplasms / Q000556:secondary*; D051379:Mice; D008807:Mice, Inbred BALB C; D015688:Oncogene Protein pp60(v-src) / Q000235:genetics*; D013755:Tetradecanoylphorbol Acetate
D015688:Oncogene Protein pp60(v-src); D013755:Tetradecanoylphorbol Acetate