Arachidonic acid metabolism in gerbil cerebra: effects of ischemia and pentobarbital.
Pentobarbital pretreatment may be used to predict biochemical events involved in ischemic brain damage following bilateral carotid artery ligations in the gerbil, since it reduces the subsequent edema and mortality. The effects of this anesthetic on the ischemia-induced modifications of cerebral arachidonic acid metabolism were investigated, in order to correlate observed alterations with tissue damage. Cerebral lipids were radiolabeled in vivo with [3H]arachidonic acid prior to 10 min of cerebral ischemia and 0-120 min of perfusion. Ischemia stimulated a 97.3% increase in unesterified [3H]arachidonate, which was due to the loss of label from choline, inositol, and ethanolamine glycerophospholipids. Tissue reperfusion stimulated further reductions in [3H]choline and [3H]inositol glycerophospholipids, while ethanolamine glycerophospholipid and triglyceride labeling increased. Inositol glycerophospholipid, but not choline glycerophospholipid, labeling returned to control level by 60 min of reperfusion. Pentobarbital pretreatment reduced the accumulation of [3H]arachidonate by 56.2% during ischemia. It increased the recovery of [3H]ethanolamine glycerophospholipids during the ischemic period and [3H]choline glycerophospholipids during the first 5 min of reperfusion. These effects accounted for the reduction of unesterified [3H]arachidonate observed during ischemia and reperfusion.
Hamm
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Department of Biological Sciences, Kent State University, OH 44242.
Dorman
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RV
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D002545:Brain Ischemia / Q000378:metabolism*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D002794:Choline / Q000378:metabolism; D004983:Ethanolamines / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005849:Gerbillinae; D007295:Inositol Phosphates / Q000378:metabolism; D008297:Male; D010424:Pentobarbital / Q000494:pharmacology*; D010477:Perfusion; D010743:Phospholipids / Q000378:metabolism; D012694:Serine / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
D001095:Arachidonic Acids; D004983:Ethanolamines; D005230:Fatty Acids, Nonesterified; D007295:Inositol Phosphates; D010743:Phospholipids; D014280:Triglycerides; D016718:Arachidonic Acid; D012694:Serine; D010424:Pentobarbital; D002794:Choline