title: FITC-labeled lipopolysaccharide: use as a probe for liposomal membrane incorporation studies.
abstract: FITC-labeled LPS from Neisseria meningitidis can be used as a probe to follow the process of LPS incorporation into liposomal membrane and to study its interaction with a bilayer. The incorporation of FITC-LPS into the bilayer was proved by physicochemical methods as well as by liposomal LPS toxicity decrease in actinomycin D-sensitized mice. Fluorescence intensity increase was observed upon the insertion of FITC-LPS into the membrane of dehydration/rehydration vesicles and vesicles obtained by co-sonication of lipid suspension and FITC-LPS. Following FITC-LPS fluorescence polarization it was shown that the substance seems to be clusterized in the liposomal membrane starting from FITC-LPS/lipid molar ratio 1:800.
authors.affiliation: USSR Cardiology Research Center, Moscow.
authors.forename: V S
authors.initials: VS
authors.lastname: Trubetskoy
authors.forename: N V
authors.initials: NV
authors.lastname: Koshkina
authors.forename: V G
authors.initials: VG
authors.lastname: Omel'yanenko
authors.forename: V L
authors.initials: VL
authors.lastname: L'vov
authors.forename: B A
authors.initials: BA
authors.lastname: Dmitriev
authors.forename: A B
authors.initials: AB
authors.lastname: Petrov
authors.forename: V P
authors.initials: VP
authors.lastname: Torchilin
mesh_terms: D000818:Animals; D002784:Cholesterol; D003609:Dactinomycin / Q000494:pharmacology; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005454:Fluorescence Polarization; D008070:Lipopolysaccharides / Q000633:toxicity; D008081:Liposomes; D051379:Mice; D008808:Mice, Inbred CBA; D009345:Neisseria meningitidis; D010713:Phosphatidylcholines; D013010:Sonication; D013050:Spectrometry, Fluorescence; D013861:Thiocyanates
chemical_list: D005452:Fluoresceins; D008070:Lipopolysaccharides; D008081:Liposomes; D010713:Phosphatidylcholines; D013861:Thiocyanates; D003609:Dactinomycin; D002784:Cholesterol; D016650:Fluorescein-5-isothiocyanate