The poly-ADP-ribosylation system of higher eukaryotes: a protein shuttle mechanism in chromatin?
The role of poly ADP-ribosylation in DNA excision repair was studied in experimental models of various complexities. In intact cells in vivo, the unfolding of chromatin during DNA excision repair apparently requires the presence of a functional poly-ADP-ribosylation system. In vitro studies involving a reconstituted poly-ADP-ribosylation system show that the enzyme poly(ADP-ribose)polymerase has the capacity to shuttle core histones on a core DNA fragment of 146 bp. Under these conditions, the polymerase operates in a strictly processive mode. Furthermore, the polymerase adapts to different shuttling targets by producing very distinct polymer patterns. We conclude that the eukaryotic poly-ADP-ribosylation system has the capacity to regulate DNA-protein interactions and this may be an essential part of the unfolding mechanism of chromatin during excision repair in vivo.
Althaus
F R
FR
University of Zürich-Tierspital, Institute of Pharmacology and Biochemistry, Switzerland.
Naegeli
H
H
Realini
C
C
Mathis
G
G
Loetscher
P
P
Mattenberger
M
M
D000818:Animals; D002843:Chromatin / Q000378:metabolism*; D004260:DNA Repair / Q000502:physiology; D008954:Models, Biological; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism*
D002843:Chromatin; D011064:Poly Adenosine Diphosphate Ribose