Recognition by restriction endodeoxyribonuclease EcoRI of octadeoxyribonucleotides containing modified sugar and base moieties.
The modified base and sugar moieties, 8-oxo-7,8-dihydroadenine (HO8A),8-methoxyadenine (MeO8A),8-methoxyguanine (MeO8G) and 9-[(2-hydroxy-1-(hydroxymethyl)-ethoxy)-methyl]-adenine (acA), were chemically introduced in place of the adenine or guanine in the octa-deoxyribonucleotides [d(GGAATTCC)] containing recognition sequence of Eco RI. to the regular DNA-fragment.
Komatsu
H
H
Department of Industrial Chemistry, Chiba Institute of Technology, Japan.
Ichikawa
T
T
Takaku
H
H
Yokoyama
S
S
Kawai
G
G
D002241:Carbohydrates / Q000737:chemistry*; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity; D013816:Thermodynamics
D002241:Carbohydrates; D009838:Oligodeoxyribonucleotides; C033068:d(GGAATTCC); D015246:Deoxyribonuclease EcoRI