Estimation of the quality of irradiated spermatozoa from cockerels using the classical method and zona-free hamster ova.
1. The quality of gamma irradiated spermatozoa from cockerels was investigated by measuring motility, oxygen consumption and ability to penetrate zona-free hamster ova. 2. Doses of 750 Gy slightly reduced motility and oxygen consumption; however, irradiated spermatozoa penetrated on average 56% (minimum 33%) of hamster ova. 3. The zona-free hamster ova test may be useful for estimating avian sperm quality.
Bednarczyk
M
M
Central Research and Development Institute of Poultry Breeding, Poznan, Poland.
Babusik
P
P
Baumgartner
J
J
Rafay
J
J
Dobalova
M
M
D000818:Animals; D002645:Chickens / Q000502:physiology*; D006224:Cricetinae; D005260:Female; D005720:Gamma Rays; D008297:Male; D008647:Mesocricetus; D010101:Oxygen Consumption / Q000528:radiation effects*; D013081:Sperm Motility / Q000528:radiation effects*; D013084:Sperm-Ovum Interactions / Q000528:radiation effects*; D013094:Spermatozoa / Q000378:metabolism / Q000502:physiology / Q000528:radiation effects*