Objective identification of hyperactivated human spermatozoa by computerized sperm motion analysis with the Hamilton-Thorn sperm motility analyser.
Sperm from 10 fertile donors were incubated for 8 h in a capacitating medium (BWW 3.5% HSA) and treated with 1.25 microliters of 2 mM Ca-ionophore solution. Sperm motion was analysed using the Hamilton-Thorn system before and after incubation and treatment. The acrosome reaction was detected with PSA-FITC labelling of the acrosome. Critical values of sperm movement parameters were defined for objective identification of hyperactive movement by the Hamilton-Thorn system. The incidence of hyperactive movement in the sperm suspensions was approximately 2%. No significant changes of hyperactive movement could be observed after the incubation period or the ionophore treatment, in contrast to a significant rise in the percentage of acrosome-reacted cells after ionophore treatment. The implications of these findings for monitoring hyperactive cells to test sperm function are discussed.
Grunert
J H
JH
Institute of Reproductive Medicine of the University, Münster, FRG.
De Geyter
C
C
Nieschlag
E
E
D000177:Acrosome / Q000502:physiology; D000001:Calcimycin / Q000494:pharmacology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D013075:Sperm Capacitation; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000502:physiology*
D000001:Calcimycin