title: Properties of a hemolysin related to the thermostable direct hemolysin produced by a Kanagawa phenomenon negative, clinical isolate of Vibrio parahaemolyticus.
abstract: A hemolytic toxin (Vp-TRH) produced by a Kanagawa phenomenon negative, clinical isolate of Vibrio parahaemolyticus was further characterized. The purified Vp-TRH showed various biological activities, such as fluid accumulation in rabbit ileal loops, increase of rabbit skin vascular permeability, and cardiotoxicity on cultured myocardial cells, all of which are essentially similar to the activities found with thermostable direct hemolysin (Vp-TDH), a pathogenic toxin produced by Kanagawa phenomenon positive V. parahaemolyticus. Immunological similarities of Vp-TRH not only to Vp-TDH but also to hemolytic toxins produced by Vibrio hollisae and Vibrio cholerae non-O1, both of which are also enteropathogens closely related to V. parahaemolyticus, were demonstrated. The amino acid composition and sequence of N-terminal amino acids of Vp-TRH were determined. These results suggest that Vp-TRH has biological and immunological characters similar to Vp-TDH, although they are distinct molecules.
authors.affiliation: Research Institute for Microbial Diseases, Osaka University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Honda
authors.forename: Y X
authors.initials: YX
authors.lastname: Ni
authors.forename: A
authors.initials: A
authors.lastname: Hata
authors.forename: M
authors.initials: M
authors.lastname: Yoh
authors.forename: T
authors.initials: T
authors.lastname: Miwatani
authors.forename: T
authors.initials: T
authors.lastname: Okamoto
authors.forename: K
authors.initials: K
authors.lastname: Goshima
authors.forename: H
authors.initials: H
authors.lastname: Takakura
authors.forename: S
authors.initials: S
authors.lastname: Tsunasawa
authors.forename: F
authors.initials: F
authors.lastname: Sakiyama
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D001834:Body Water / Q000378:metabolism; D002199:Capillary Permeability; D002478:Cells, Cultured; D003429:Cross Reactions; D006460:Hemolysin Proteins / Q000032:analysis / Q000276:immunology / Q000633:toxicity*; D006801:Humans; D005779:Immunodiffusion; D051379:Mice; D008969:Molecular Sequence Data; D009200:Myocardial Contraction; D011817:Rabbits; D013696:Temperature; D014736:Vibrio parahaemolyticus / Q000302:isolation & purification
chemical_list: D006460:Hemolysin Proteins