Identification of the serum-responsive transcription initiation site of the zinc finger gene Krox-20.
The Krox-20 gene is rapidly and transiently induced when quiescent 3T3 cells are stimulated to reenter the proliferative cycle. We identified the major serum-responsive transcription initiation site and found that it differs from the initiation sites previously identified for the Krox-20 gene. Transcripts from the major serum-responsive initiation site increased at least 40-fold in serum-stimulated cells compared with logarithmically growing cells.
Cortner
J
J
McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.
Farnham
P J
PJ
D000818:Animals; D001483:Base Sequence; D001769:Blood; D002478:Cells, Cultured; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000235:genetics*; D051767:Early Growth Response Protein 2; D005786:Gene Expression Regulation; D005796:Genes; D007700:Kinetics; D008667:Metalloproteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects
D003470:Culture Media; D004268:DNA-Binding Proteins; D051767:Early Growth Response Protein 2; C497327:Egr2 protein, mouse; D008667:Metalloproteins; D012333:RNA, Messenger; D014157:Transcription Factors; D003513:Cycloheximide