Significant increase of 8-hydroxydeoxyguanosine in liver DNA of rats following short-term exposure to the peroxisome proliferators di(2-ethylhexyl)phthalate and di(2-ethylhexyl)adipate.
8-Hydroxydeoxyguanosine (8-OH-dG) levels were examined in liver and kidney DNA after di(2-ethylhexyl)phthalate (DEHP), di(2-ethylhexyl)adipate (DEHA) and phthalic anhydride administration to male 6-week-old F-344 rats in the diet at concentrations of 1.2, 2.5 and 1.5%, respectively. Significant increases in 8-OH-dG levels were observed only in the liver (target organ of DEHP and DEHA carcinogenesis) DNA after 1 and 2 weeks of treatment with DEHP and DEHA, respectively. The results suggest the involvement of oxidative DNA damage in hepatocarcinogenesis by peroxisome proliferators.
Takagi
A
A
Division of Toxicology, National Institute of Hygienic Sciences, Tokyo.
Sai
K
K
Umemura
T
T
Hasegawa
R
R
Kurokawa
Y
Y
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000272:Adipates / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism*; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D004051:Diethylhexyl Phthalate / Q000008:administration & dosage / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced; D008297:Male; D008830:Microbodies / Q000187:drug effects* / Q000648:ultrastructure; D010795:Phthalic Acids / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344
D000272:Adipates; D002273:Carcinogens; D010795:Phthalic Acids; C013966:dioctyl adipate; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D004051:Diethylhexyl Phthalate; D003849:Deoxyguanosine