title: Messenger RNA levels of guanine nucleotide-binding proteins are reduced in the ventricle of cardiomyopathic hamsters.
abstract: The expression of guanine nucleotide-binding protein (G-protein) genes (Gs alpha, Go alpha, Gi alpha 1, Gi alpha 2, and Gi alpha 3) was examined in the ventricle of cardiomyopathic Syrian hamsters of the Bio14.6 strain (10-35 weeks old). Northern blot analysis of total cellular RNA revealed that all G-protein genes except Gi alpha 1 were expressed in the ventricle of Syrian hamsters. Gs alpha and Gi alpha 2 genes were abundantly expressed. The expression levels of the Gs alpha and Gi alpha 2 messenger RNAs in Bio14.6 ventricles were lower than the levels in ventricles of the F1B hamster strain; the abundance of Go alpha and Gi alpha 3 messenger RNAs did not change markedly. Moreover, the messenger RNA levels of Gs alpha and Gi alpha 2 decreased as the stage of cardiomyopathy progressed. Since G-proteins are linked to adenylate cyclase, these alterations of G-protein messenger RNA levels may be related to reduced contractility of cardiomyopathic heart.
authors.affiliation: Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Katoh
authors.forename: I
authors.initials: I
authors.lastname: Komuro
authors.forename: F
authors.initials: F
authors.lastname: Takaku
authors.forename: H
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Yazaki
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D009202:Cardiomyopathies / Q000378:metabolism*; D006224:Cricetinae; D019204:GTP-Binding Proteins / Q000235:genetics*; D006352:Heart Ventricles; D009206:Myocardium / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*; D012016:Reference Values
chemical_list: D012333:RNA, Messenger; D019204:GTP-Binding Proteins