Enzyme immunoassay for flecainide.
In order to develop an enzyme immunoassay (ELISA) for measurement of plasma flecainide levels, we first synthesized flecainide-hemisuccinate-BSA as immunogen. A highly specific antiserum was then raised in rabbits. Enzyme-labelled flecainide was prepared by the mixed anhydride method using hemifumarate-flecainide and horse radish peroxidase. The limit of detection was 10 ng/ml, the method was linear up to 1,000 micrograms/l. The intra and inter-assay variation was 5.1-7.8%. Cross reactivity with various metabolites of flecainide or some chemically related drugs was less than 0.1%. By contrast the cross reaction with some structural analogues achieved 25%. The importance of parts of the aromatic ring structure in immunogenic properties of this drug is discussed.
Freche
J P
JP
Centre du Medicament, URA CNRS no. 597, Nancy, France.
Coudert
G
G
Wellman
M
M
Siest
G
G
D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D005424:Flecainide / Q000097:blood* / Q000737:chemistry / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards / Q000706:statistics & numerical data; D015394:Molecular Structure; D012710:Serum Albumin, Bovine / Q000276:immunology; D013387:Succinic Anhydrides / Q000276:immunology
D000906:Antibodies; D000941:Antigens; D013387:Succinic Anhydrides; D012710:Serum Albumin, Bovine; D005424:Flecainide