Mastoparan, a wasp venom, activates platelets via pertussis toxin-sensitive GTP-binding proteins.
Mastoparan induced limited release of serotonin from intact human platelets, while neither intracellular calcium ion elevation nor arachidonic acid mobilization was observed. Cytolysis induced by mastoparan was negligible in the concentration range that induced serotonin release. In digitonin-permeabilized cells, mastoparan induced Ca(++)-independent release of serotonin and Ca(++)-dependent arachidonic acid release. Both serotonin release and arachidonic acid release were reduced by pertussis toxin, suggesting that platelet activation induced by mastoparan is mediated by GTP-binding proteins.
Ozaki
Y
Y
Department of Clinical and Laboratory Medicine, Yamanashi Medical College, Japan.
Matsumoto
Y
Y
Yatomi
Y
Y
Higashihara
M
M
Kariya
T
T
Kume
S
S
D001095:Arachidonic Acids / Q000378:metabolism; D001514:Bee Venoms / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D010455:Peptides; D037342:Pertussis Toxin; D015539:Platelet Activation / Q000187:drug effects*; D012701:Serotonin / Q000378:metabolism; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*; D014862:Wasp Venoms / Q000008:administration & dosage / Q000494:pharmacology*
D001095:Arachidonic Acids; D001514:Bee Venoms; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D010566:Virulence Factors, Bordetella; D014862:Wasp Venoms; D012701:Serotonin; C037500:mastoparan; D007770:L-Lactate Dehydrogenase; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins