Structure and expression of a tobacco beta-1,3-glucanase gene.
We determined the primary structure of a tobacco beta-1,3-glucanase gene. The beta-1,3-glucanase gene has a single large intron, and the intron separates coding regions of the signal peptide and the mature enzyme. Analysis of the 5'-flanking region sequence revealed an 11 bp GC-rich element with perfect homology to the putative regulatory sequence of tobacco chitinase genes. RNA blot analysis showed that levels of mRNAs of beta-1,3-glucanase and chitinase are coordinately increased in response to ethylene and salicylic acid. Accumulation of beta-1,3-glucanase mRNA in suspension-cultured cells is rapidly induced at late logarithmic growth phase. Members of the tobacco beta-1,3-glucanase gene families are classified into two subfamilies. One of the subfamilies appeared to be transcriptionally inactive.
Ohme-Takagi
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Laboratory of Plant Molecular Biology, Fermentation Research Institute, Ibaraki, Japan.
Shinshi
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D000595:Amino Acid Sequence; D001483:Base Sequence; D002480:Cellulase / Q000096:biosynthesis / Q000235:genetics*; D002688:Chitinases / Q000235:genetics; D005030:Ethylenes / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D017343:Genes, Plant; D008969:Molecular Sequence Data; D005810:Multigene Family; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D010947:Plants, Toxic; D012459:Salicylates / Q000494:pharmacology; D020156:Salicylic Acid; D012689:Sequence Homology, Nucleic Acid; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic
D005030:Ethylenes; D010940:Plant Proteins; D012459:Salicylates; C053376:pathogenesis-related proteins, plant; C036216:ethylene; D002688:Chitinases; D002480:Cellulase; D020156:Salicylic Acid