title: Particles from dialysis tubing stimulate interleukin-1 secretion by macrophages.
abstract: Loading of tissue macrophages with dialysis-tubing-derived particles may occur during chronic haemodialysis. Previous studies have demonstrated that these particle-laden macrophages release significant quantities of prostaglandins. In these experiments, the effects of dialysis-tubing-particle loading on the release of the central inflammatory mediator, interleukin 1 (IL 1), was examined. Rats received daily injections of silicone or polyvinylchloride (PVC) particles, and were compared to animals given saline alone. The silicone and PVC groups received a total of 3 x 10(9) particles over a 4-week period. Non-stimulated peritoneal macrophages from control animals released a median of 4.1 (range 1.2-10.3) U IL 1 per 10(6) cells. In contrast, macrophages from silicone- and PVC-loaded animals spontaneously released high levels of IL 1 (median 21.8; range 10-36.7) and 94 (range 36-336) U per 10(6) cells respectively). Following in vitro stimulation with bacterial lipopolysaccharide (LPS), peritoneal macrophages from silicone- and PVC-treated animals released large amounts of IL 1 (median 538 (range 359-2017) U and median 653 (range 326-1134) U per 10(6) cells, respectively) as compared to LPS-stimulated macrophages from control animals (median 332 (range 130-306) U per 10(6) cells]. Zymosan or LPS stimulation of splenic cells from silicone- and PVC-loaded animals also secreted increased quantities of IL 1 as compared to controls. The chronic loading of tissue macrophages in dialysis patients with tubing-derived particles may result in augmented release of IL 1, with subsequent activation of inflammatory processes.
authors.affiliation: Department of Internal Medicine, University of Heidelberg, FRG.
authors.forename: J
authors.initials: J
authors.lastname: Bommer
authors.forename: T
authors.initials: T
authors.lastname: Weinreich
authors.forename: D H
authors.initials: DH
authors.lastname: Lovett
authors.forename: R
authors.initials: R
authors.lastname: Bouillon
authors.forename: E
authors.initials: E
authors.lastname: Ritz
authors.forename: D
authors.initials: D
authors.lastname: Gemsa
mesh_terms: D000818:Animals; D046508:Culture Techniques; D003956:Dialysis / Q000295:instrumentation*; D004195:Disease Models, Animal; D007375:Interleukin-1 / Q000378:metabolism*; D007376:Interleukin-2 / Q000378:metabolism*; D008099:Liver / Q000187:drug effects; D008168:Lung / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011143:Polyvinyl Chloride / Q000494:pharmacology; D051381:Rats; D012828:Silicones / Q000494:pharmacology; D013154:Spleen / Q000187:drug effects
chemical_list: D007375:Interleukin-1; D007376:Interleukin-2; D012828:Silicones; D011143:Polyvinyl Chloride