Effect of temperature on the lactose hydrolytic capacity of a lactase derived from Kluyveromyces lactis.
In vitro studies of lactose hydrolysis in milk with 20-125 neutral lactase units (NLUs) carried out at 38.0 degrees C for 15 min with a beta-galactosidase derived from Kluyveromyces lactis (Lactaid, Lactaid Inc, Pleasantville, NJ) resulted in 85-95% of the hydrolysis observed with standard incubation conditions (24 h at 4-5 degrees C with 1000 NLU/L). Thirty-three lactose-maldigesting Guatemalan subjects, 16 children and 17 adults, were challenged with oral doses of lactose in milk (children aged less than 12 mo, 2 g/kg body wt; children aged 12-24 mo, 15 g/kg body wt; older children and adults, 18 g/kg body wt) preincubated for 20 min at 38 +/- 0.5 degrees C with 50-125 NLU Lactaid. Under these conditions the subjects consumed milk without presenting any signs of intolerance. Furthermore, their breath-hydrogen excretion showed a 91-93% reduction when compared with a similar load of milk containing nonhydrolyzed lactose.
Schneider
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RE
Gastroenterology and Nutrition Service, Common Diseases General Hospital of the Guatemalan Social Security Institute, Guatemala City.
Corona
E
E
Rosales
F
F
Schneider
F E
FE
Rodriguez
O
O
Pineda
O
O
D000328:Adult; D000818:Animals; D005260:Female; D005696:Galactosidases / Q000378:metabolism*; D006801:Humans; D006859:Hydrogen / Q000032:analysis; D006868:Hydrolysis; D007223:Infant; D007716:Kluyveromyces / Q000201:enzymology*; D007785:Lactose / Q000378:metabolism*; D007787:Lactose Intolerance / Q000178:diet therapy*; D008297:Male; D008875:Middle Aged; D008892:Milk; D051381:Rats; D004718:Saccharomycetales / Q000201:enzymology*; D013696:Temperature; D001616:beta-Galactosidase / Q000378:metabolism*
D006859:Hydrogen; D005696:Galactosidases; D001616:beta-Galactosidase; D007785:Lactose