Chemical synthesis of oligoribonucleotides for structural studies.
Large-scale synthesis of oligoribonucleotides has been performed successfully on a solid support by the phosphoramidite approach using levulinyl and tetrahydrofuranyl protection for the 5'- and 2'-hydroxyl groups respectively. A hexamer containing inosine and four fragments of a hammerhead-type ribozyme have been synthesized on a 10 mumol scale for structural studies by NMR spectroscopy and X-ray crystallography.
Sasaki
T
T
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Iwai
S
S
Tsuda
S
S
Hikichi
K
K
Ohtsuka
E
E
D001483:Base Sequence; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D016337:RNA, Catalytic / Q000737:chemistry
D009843:Oligoribonucleotides; D016337:RNA, Catalytic