Evaluation of ELISA for detection of Giardia lamblia-specific copro-antigen employing monospecific antibodies.
An enzyme linked immunosorbent assay (ELISA) system, using monospecific antibodies for the detection of Giardia lamblia specific 66 kDa copro-antigen has been developed and evaluated. The assay detected the antigen in stool eluates of all the 24 microscopically confirmed cases of giardiasis and in 17 (68%) of the 25 microscopy-negative clinically suspected cases of giardiasis. None of stool eluates from 20 subjects infected with other protozoal/helminthic intestinal parasites or from 20 apparently healthy subjects had G. lamblia-specific copro-antigen. The ELISA employing monospecific antibodies is a sensitive and specific tool for the diagnosis of giardiasis and is especially useful for confirming microscopy-negative suspected cases of giardiasis.
Dutt
P
P
Department of Experimental Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Vinayak
V K
VK
D000818:Animals; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015153:Blotting, Western; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D005188:False Negative Reactions; D005243:Feces / Q000469:parasitology; D005872:Giardia / Q000276:immunology*; D005873:Giardiasis / Q000175:diagnosis*; D006801:Humans
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan