Mammary tumor immunoscintigraphy in rats: the use of 131I-anti-estriol 3-sulfate antibody.
The scintigraphic imaging of mammary tumors with anti-estriol 3-sulfate (E3 3-S) antibody was studied in rats. A chemical carcinogen, 7,12-dimethylbenz(a)anthracine (DMBA), induced mammary tumors in Sprague-Dawley female rats. Highly specific anti-E3 3-S antibody was prepared and radioiodinated by [131I]NaI using the chloramine-T method. At 24 h after administration of 131I-anti-E3 3-S antibody, goat anti-guinea pig immunogloblin G (IgG) was injected as the second antibody (SA) and nuclear scintigraphy was performed. Mammary tumors were clearly visualized following SA injection.
Shimura
N
N
Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
Komori
Y
Y
Tanaka
T
T
Kubodera
A
A
Oyamada
H
H
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D000906:Antibodies / Q000493:pharmacokinetics; D004964:Estriol / Q000276:immunology* / Q000031:analogs & derivatives; D005260:Female; D006041:Goats / Q000276:immunology; D006168:Guinea Pigs; D007074:Immunoglobulin G / Q000008:administration & dosage; D007457:Iodine Radioisotopes; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000000981:diagnostic imaging* / Q000276:immunology; D011877:Radionuclide Imaging; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D000906:Antibodies; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; C027974:estriol 3-sulfate; D015127:9,10-Dimethyl-1,2-benzanthracene; D004964:Estriol