title: Immobilized carbonic anhydrase in a membrane lung for enhanced CO2 removal.
abstract: The potential use of carbonic anhydrase (CA), immobilized within membrane lungs, to accelerate the removal of CO2 from blood was investigated. Using a variation on the technique of Chang, 500 micrograms/mL of CA was encapsulated in 5-20 microns cellulose nitrate microcapsules which were then immobilized onto a 0.1 m2 silicone rubber membrane. Using an in vitro test circuit, 0.68 mmol/m2/min of CO2 was removed from venous blood through an untreated membrane at steady state. With the CA-immobilized membrane, CO2 removal was enhanced to 1.09 mmol/m2/min. This technique has the potential of greatly improving the CO2 efficiency of commercial membrane lungs for ECCOR.
authors.affiliation: Department of Chemical Engineering, Wayne State University, Detroit, MI.
authors.forename: S O
authors.initials: SO
authors.lastname: Salley
authors.forename: J Y
authors.initials: JY
authors.lastname: Song
authors.forename: G C
authors.initials: GC
authors.lastname: Whittlesey
authors.forename: M D
authors.initials: MD
authors.lastname: Klein
mesh_terms: D002245:Carbon Dioxide / Q000097:blood*; D002256:Carbonic Anhydrases; D004800:Enzymes, Immobilized; D004867:Equipment Design; D015199:Extracorporeal Membrane Oxygenation / Q000295:instrumentation*; D006801:Humans; D010107:Oxygenators, Membrane; D010316:Particle Size; D013499:Surface Properties
chemical_list: D004800:Enzymes, Immobilized; D002245:Carbon Dioxide; D002256:Carbonic Anhydrases