Mutational activation of H-ras and K-ras genes is absent in N-nitroso-N-methylurea-induced liver tumors in rats.
We examined mutational activation of H- and K-ras genes in hyperplastic nodules and hepatocellular carcinomas induced by N-nitroso-N-methylurea or diethylnitrosamine using the polymerase chain reaction, followed by dot-blot hybridization. No mutational activation was detected in these hepatic lesions. The results indicate that low incidence of the activation of H- and K-ras genes in rat liver tumors is due to the organ specificity rather than the nature of the carcinogens used.
Sakai
H
H
Department of Pathology, Asahikawa Medical College.
Ogawa
K
K
D000818:Animals; D004052:Diethylnitrosamine; D011905:Genes, ras / Q000187:drug effects*; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000235:genetics*; D008297:Male; D008770:Methylnitrosourea; D009154:Mutation; D016133:Polymerase Chain Reaction; D051381:Rats; D011916:Rats, Inbred F344
D004052:Diethylnitrosamine; D008770:Methylnitrosourea