Carbonic anhydrase III in equine tissues and sera determined by a highly sensitive enzyme-immunoassay.
A sensitive sandwich enzyme immunoassay (EIA) for measuring equine carbonic anhydrase III (CA-III) was established using a microplate as a solid-phase and peroxidase as a labelling enzyme. The assay can detect concentrations as low as 5 ng/ml using 20 microliters of sample sera. Within-run coefficients of variation obtained using standard equine CA-III were less than 5 per cent. CA-III levels in equine serum ranged from 5 to 50 ng/ml (n = 370), and apparently abnormal levels of CA-III from 100 to 1900 ng/ml (n = 27) were observed. The concentrations of immunoreactive CA-III in the extracts of various equine tissues were also determined; it was present at high concentrations in skeletal muscle and liver and to a much lesser extent in the thymus. Other tissues contained much smaller amounts.
Nishita
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Laboratory of Veterinary Physiology, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
Matsushita
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D000818:Animals; D002256:Carbonic Anhydrases / Q000032:analysis* / Q000097:blood; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D006736:Horses / Q000097:blood / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D008099:Liver / Q000201:enzymology; D008297:Male; D009132:Muscles / Q000201:enzymology; D011237:Predictive Value of Tests; D013950:Thymus Gland / Q000201:enzymology
D002256:Carbonic Anhydrases