title: Genes for tryptophan biosynthesis in the archaebacterium Haloferax volcanii.
abstract: Recent technical advances permit direct genetic approaches for isolating genes and mapping auxotrophic mutations in the halophilic archaebacterium (Archaea) Haloferax volcanii. Twenty-nine mutations in tryptophan biosynthesis mapped to two separate chromosomal locations. DNA sequencing of one gene cluster shows a unique gene order (trpCBA) and unusual potential secondary structures in the 5'-flanking region.
authors.affiliation: Department of Biochemistry, Dalhousie University, Halifax, NS, Canada.
authors.forename: W L
authors.initials: WL
authors.lastname: Lam
authors.forename: A
authors.initials: A
authors.lastname: Cohen
authors.forename: D
authors.initials: D
authors.lastname: Tsouluhas
authors.forename: W F
authors.initials: WF
authors.lastname: Doolittle
mesh_terms: D000466:Alkadienes; D000595:Amino Acid Sequence; D001105:Archaea / Q000235:genetics*; D001419:Bacteria / Q000235:genetics*; D001483:Base Sequence; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D003360:Cosmids; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014169:Transformation, Bacterial; D014364:Tryptophan / Q000096:biosynthesis*
chemical_list: D000466:Alkadienes; D003062:Codon; D014364:Tryptophan