[Inhibiting effect of mitoxantrone on cell cycle progression of Chinese hamster ovary cells].
The effect of Mitoxantrone, a Potential anticancer chemotherapeutic agent, on the cell cycle progression of Chinese Hamster ovary (CHO) cells was investigated by microspectrophotometry. CHO cells incubated with the agent for either 30 min or 24 h were inhibited, to various degrees, from proliferation. The inhibition appeared in dose dependent fashion. A 10-fold dose of the drug was required for the 30 min group, as compared to the 24h group, to develop an equivalent inhibiting effect. When exponentially growing cells were treated with Mitoxantrone for 30 min, washed free of drug, and cultured in fresh medium for another 23.5 h a dose of 0.01 micrograms/ml had little or no effect on the distribution of CHO cells throughout the cell cycle at any point. However, a dose of 0.10-1.00 micrograms/ml was sufficient to cause a decrease of cells in G1 and an accumulation of cells in G2. Block cells had abnormally large nucleus.
Hu
L
L
Department of Biology Chongqing, University of Medical Sciences.
Yang
F
F
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D015591:Microspectrophotometry; D008942:Mitoxantrone / Q000494:pharmacology*; D010053:Ovary / Q000166:cytology / Q000187:drug effects*
D008942:Mitoxantrone