Transduction of a plasmid containing the bacteriophage D3 cos site in Pseudomonas aeruginosa.
Plasmids harboring the cos sequences of bacteriophage D3 can be transferred, by bacteriophage D3, into Pseudomonas aeruginosa by a mechanism which is insensitive to DNase. Transducing activity was separated from the plaque-forming particles by CsCl equilibrium gradient centrifugation. Restriction endonuclease digestion patterns suggest that the transducing particles contain plasmid concatemers.
Sharp
R
R
Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada.
Gertman
E
E
Farinha
M A
MA
Kropinski
A M
AM
D001435:Bacteriophages / Q000235:genetics*; D003360:Cosmids; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D014161:Transduction, Genetic