Comparison between the L-DOPA histofluorescence procedure and the indirect immunofluorescence with anti-T6 and -HLA-DR monoclonal antibodies in visualizing Langerhans cells of human epidermis.
In order to assess the reliability of the L-DOPA histofluorescence (HF) procedure in visualizing the Langerhans cells (LC) of human epidermis, serial sections of human normal skin samples have been incubated with L-DOPA and alternatively processed for the L-DOPA HF and the indirect IF with anti-T6 and anti-HLA-DR monoclonal antibodies. Results demonstrated a good correlation of LC labelling; comparison between the number of L-DOPA positive (T6 positive and L-DOPA positive) HLA-DR positive dendritic cells did not show statistically significant differences. Therefore, the L-DOPA HF represents a valuable method for detecting LC in the human normal epidermis.
Crivellato
E
E
Istituto di Anatomia Umana Normale, Facoltà di Medicina e Chirurgia, Università di Trieste, Italy.
Damiani
D
D
Mallardi
F
F
D000328:Adult; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D000078404:Epidermal Cells; D005260:Female; D005455:Fluorescent Antibody Technique; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D007801:Langerhans Cells / Q000166:cytology* / Q000276:immunology; D007980:Levodopa / Q000032:analysis*; D008297:Male; D008856:Microscopy, Fluorescence / Q000379:methods
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D006684:HLA-DR Antigens; D007980:Levodopa