[Embryotoxicity of fenbendazole in Paracentrotus lividus].
The aim of the present work was to evaluate the embryotoxicity of Fenbendazole, a benzimidazole carbamate-derived anthelmintic drug widely employed in Veterinary Medicine, by using the embryonal development of Paracentrotus lividus (sea urchin) as a experimental model. Embryos were obtained by in vitro eggs fertilization and cultured in seawater. Five embryo suspensions were added by Fenbendazole reaching a final concentration of 5 micrograms/l, 7.5 micrograms/l, 10 micrograms/l, 12.5 micrograms/l and 25 micrograms/l; a suspension was kept drug-free as a control. Embryo development was evaluated by microscopical examination of suspensions at 3 and 40 hours. Our results show that a concentration of 5 micrograms/l of the drug determines a considerable delay of the embryonal development in the 95 percent of the elements observed, and a concentration of 25 micrograms/l produces a block of the embryogenesis at the phase of morula and blastula in all embryos. Results confirm that the effects observed are probably due to an extended inhibition of several enzyme complexes of the embryo cells.
Deiana
L
L
Cattedra di Analisi Biologiche, Facoltà di Medicina, Università degli Studi di Sassari.
Congiu
A M
AM
Carru
C
C
Pes
G M
GM
Arru
G
G
D000818:Animals; D001755:Blastocyst / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D004625:Embryo, Nonmammalian / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005273:Fenbendazole / Q000494:pharmacology / Q000633:toxicity*; D008870:Microtubules / Q000187:drug effects; D009028:Morula / Q000187:drug effects; D012617:Sea Urchins / Q000196:embryology*
D004791:Enzyme Inhibitors; D005273:Fenbendazole
Embriotossicità del fenbendazolo in Paracentrotus lividus.