title: Detection of N-acetylcysteine, cysteine and their disulfides in urine by liquid chromatography with a dual-electrode amperometric detector.
abstract: A method for the determination of N-acetylcysteine, cysteine and their disulfides in urine is described. The thiols and disulfides are separated by reversed-phase ion-pair chromatography with octyl sodium sulfate as the ion-pairing reagent and detected with a dual-electrode amperometric detector using Au/Hg amalgam electrodes. Both the thiols and disulfides are detected with this system. In addition, dimers and mixed disulfides can be detected individually.
authors.affiliation: Department of Chemistry, University of Kansas, Lawrence 66045.
authors.forename: J A
authors.initials: JA
authors.lastname: Stenken
authors.forename: D L
authors.initials: DL
authors.lastname: Puckett
authors.forename: S M
authors.initials: SM
authors.lastname: Lunte
authors.forename: C E
authors.initials: CE
authors.lastname: Lunte
mesh_terms: D000111:Acetylcysteine / Q000652:urine*; D002853:Chromatography, Liquid; D003545:Cysteine / Q000652:urine*; D004220:Disulfides / Q000652:urine*; D004563:Electrochemistry; D006801:Humans
chemical_list: D004220:Disulfides; D003545:Cysteine; D000111:Acetylcysteine