title: Yeast artificial chromosomes: tools for mapping and analysis of complex genomes.
abstract: Libraries of yeast artificial chromosomes (YACs) are representative of complex genomes, and typical YACs contain single fragments of DNA that are up to a megabase or more in size, are stable during growth, and are faithful to genomic DNA. Such YACs may permit (1) the use of complete gene units for a number of research and medical purposes; and (2) the assembly of chromosome-sized contigs in a single map that unifies genetic and physical data.
authors.affiliation: Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110.
authors.forename: D
authors.initials: D
authors.lastname: Schlessinger
mesh_terms: D000818:Animals; D002107:Caenorhabditis / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods*; D015825:Chromosomes, Fungal / Q000648:ultrastructure; D003001:Cloning, Molecular; D004331:Drosophila melanogaster / Q000235:genetics; D005786:Gene Expression Regulation; D030342:Genetic Diseases, Inborn / Q000235:genetics; D005822:Genetic Vectors; D015894:Genome, Human; D015698:Genomic Library; D006801:Humans; D012441:Saccharomyces cerevisiae / Q000235:genetics*