[Rapid identification of Mycobacterium tuberculosis and other mycobacteria with Mycobacterium tuberculosis whole chromosomal DNA probe].
The dot-blots containing DNA isolated from nonmycobacterial and mycobacterial microorganisms were hybridized with 32P-labeled M. tuberculosis whole chromosomal DNA at the various temperatures. The probe did not cross-hybridize to DNA of nonmycobacterial microorganisms (E. coli, Plasmid pUC19, Nocardia asteriodes), nor with DNA from all mycobacteria tested except M. bovis BCG under the higher temperature conditions. Microorganisms could also be directly spotted and lysed on nitrocellulose filters and used for hybridization thus making this technique suitable for clinical diagnosis.
Wu
X
X
Tuberculosis Center, 309th Hospital of PLA, Beijing.
Zhuang
Y
Y
Huang
R
R
Liang
H
H
D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000302:isolation & purification
D015342:DNA Probes; D004269:DNA, Bacterial