Studies on peroxidation processes of model membranes and synaptosomes: role of phosphatidic acid.
Dipalmitoylphosphatidic acid (DPPA) was found to exert a strong inhibitory effect on Fe-induced peroxidation of arachidonic acid inserted into liposomal dipalmitoylphosphatidylcholine (DPPC) vesicles. This inhibition was quite effective both below and above the phase transition temperature of the liposomes. Moreover, we demonstrated the antiperoxidative activity of phosphatidic acid (PA) in synaptosomal membranes. PA enriched synaptosomes were prepared by the stimulation of the endogenous phospholipase D activity or by the incubation of the synaptosomes with Streptomyces chromofuscus phospholipase D. The possible contribution of PA to the in vivo defense mechanism against free radical-induced damage is discussed.
Viani
P
P
Dept. of Medical Chemistry and Biochemistry, Faculty of Medicine, University of Milano, Italy.
Cervato
G
G
Fiorilli
A
A
Rigamonti
E
E
Cestaro
B
B
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D005296:Ferrous Compounds / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D008566:Membranes / Q000378:metabolism*; D008954:Models, Biological; D010712:Phosphatidic Acids / Q000378:metabolism; D010739:Phospholipase D / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013574:Synaptosomes / Q000378:metabolism*
D001095:Arachidonic Acids; D005296:Ferrous Compounds; D010712:Phosphatidic Acids; C051140:iron(II)-ascorbic acid complex; D016718:Arachidonic Acid; D010739:Phospholipase D; D001205:Ascorbic Acid