title: Stimulation of the respiratory burst and promotion of bacterial killing in human granulocytes by intravenous immunoglobulin preparations.
abstract: We have examined the effect of two i.v. immunoglobulin preparations on the metabolic and functional activities of neutrophil granulocytes from the peripheral blood. Production of superoxide anion (O2-) by granulocytes was measured through superoxide dismutase inhibitable reduction of ferricytochrome C after incubation of cells for various times together with immunoglobulin (concentration ranging from 0.25 to 5.0 mg/ml). The results showed dose-dependent response of O2- production independent of the incubation time. Granulocytes containing ingested Staphylococcus aureus released a significantly (P less than 0.001) larger amount of O2- and killed a higher number (P less than 0.001) of viable bacteria in the presence of 5 mg/ml immunoglobulin than did cells incubated in the absence of extracellular i.v. immunoglobulin. These data raise the possibility that immunoglobulin concentrates for i.v. use may enhance the anti-bacterial activities of phagocytic cells through direct stimulation of the respiratory burst. Inflammatory reactions observed during i.v. immunoglobulin infusion in hypo- or agammaglobulinaemic patients may also be related to phagocytic cell activation.
authors.affiliation: Department of Paediatrics, University School of Medicine, Debrecen, Hungary.
authors.forename: L
authors.initials: L
authors.lastname: Maródi
authors.forename: A
authors.initials: A
authors.lastname: Kalmár
authors.forename: L
authors.initials: L
authors.lastname: Karmazsin
mesh_terms: D006098:Granulocytes / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000494:pharmacology*; D016756:Immunoglobulins, Intravenous; D010101:Oxygen Consumption; D010587:Phagocytosis; D013211:Staphylococcus aureus / Q000276:immunology*
chemical_list: D007074:Immunoglobulin G; D016756:Immunoglobulins, Intravenous