Gas chromatography of C19 and 17-oxogenic steroids in urine.
17-oxogenic steroids have been analysed by gas chromatography (GC) with capillary column and flame ionization detector. The best separation of steroid products was gained after ethyloxime-trimethylsilyl ether formation. The value of the GC method as a screening procedure for patients with suspected adrenal disease is illustrated in selected cases where the diagnosis was equivocal from data derived by RIA analysis of plasma steroids.
Honour
J W
JW
Department of Chemical Pathology, University College, London, UK.
Tsang
W M
WM
Patel
H
H
D015065:17-Hydroxycorticosteroids / Q000652:urine*; D000293:Adolescent; D000307:Adrenal Gland Diseases / Q000652:urine; D000328:Adult; D002675:Child, Preschool; D002849:Chromatography, Gas / Q000379:methods*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D010091:Oximes / Q000378:metabolism; D011863:Radioimmunoassay; D014297:Trimethylsilyl Compounds / Q000378:metabolism
D015065:17-Hydroxycorticosteroids; D010091:Oximes; D014297:Trimethylsilyl Compounds