[Effect of phospholipase A2 on pancreatic parenchymal necrosis in acute pancreatitis in rats].
The effect and mechanism of phospholipase A2 (PLA2) on pancreatic parenchymal necrosis in acute pancreatitis (AP) in rats were studied. Normal saline (NS), PLA2, phosphatidylcholine (PC), and PLA2 mixed PC were respectively injected into the biliopancreatic duct of the rat. The mixture of PLA2 and PC was found to result in necrotizing AP in the rat in 12 hours after injection of the reagent. The lysophosphatidylcholine (LPC) percentage in the amount of PC and LPC and the extent of pancreatic necrosis in this group increased much more significantly than those in other three groups (over about 4 and 15 times). There is evidently positive correlation (r = 0.9001, P less than 0.01) between LPC percentage and necrosis extent. The intraductal injection of PLA2, as well as NS or PC, failed to induce severe morphological changes in the pancreases. These results suggest that PC in bile is necessary in the pathogenesis of biliary pancreatitis and that LPC originated from bile PC by PLA2 may be the initiator in pancreatic parenchymal necrosis in AP.
Chen
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N
Xiong
Q
Q
D000208:Acute Disease; D000818:Animals; D005260:Female; D008244:Lysophosphatidylcholines; D008297:Male; D009336:Necrosis; D010179:Pancreas / Q000473:pathology; D010195:Pancreatitis / Q000139:chemically induced* / Q000473:pathology; D010741:Phospholipases A; D054467:Phospholipases A2; D051381:Rats; D011919:Rats, Inbred Strains
D008244:Lysophosphatidylcholines; D010741:Phospholipases A; D054467:Phospholipases A2