Characterization of a putative promoter region of the human poly(ADP-ribose) polymerase gene: structural similarity to that of the DNA polymerase beta gene.
The 5'-flanking region of the human poly(ADP-ribose) polymerase gene was isolated and characterized. The nucleotide sequence of a part of the poly(ADP-ribose) polymerase gene completely matched that of the cDNA. The transcriptional initiation sites (cap sites) of this gene, located about 166-bp upstream from the translational initiation site, were identified by S1 mapping analysis. Neither CAAT box nor TATA box was found within 500-bp upstream from the cap sites of poly(ADP-ribose) polymerase gene. The 200-bp immediately upstream of the cap site had a high G+C content (76.5%) and contained double repeats of the sequence CCGCCC, putative Sp1 binding sites, and a palindromic structure. The 5'-flanking region of poly(ADP-ribose) polymerase gene also showed promoter activity in chloramphenicol acetyltransferase assay and structural similarity to that of DNA polymerase beta gene.
Ogura
T
T
Biochemistry Division, National Cancer Center Research Institute, Tokyo, Japan.
Nyunoya
H
H
Takahashi-Masutani
M
M
Miwa
M
M
Sugimura
T
T
Esumi
H
H
D001483:Base Sequence; D003360:Cosmids; D004247:DNA / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D004256:DNA Polymerase I / Q000235:genetics*; D005796:Genes; D015698:Genomic Library; D006801:Humans; D007438:Introns; D007962:Leukocytes / Q000201:enzymology; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D011065:Poly(ADP-ribose) Polymerases / Q000097:blood / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D004247:DNA; D011065:Poly(ADP-ribose) Polymerases; D004256:DNA Polymerase I