Immunoglobulin heavy chain and alpha T-cell receptor genes rearrange in T-cell leukemia associated with a t(14;14) (q11.2;q32) translocation.
We report here a cytogenetic and molecular analysis of two cases of T-cell leukemia with t(14;14) (q11.2;q32). Through in situ hybridization and Southern blotting, using radioactively labeled immunoglobulin heavy chain (IGH) and alpha T-cell receptor (TCRA) gene probes, we found in both tumors that the loci of both IGH and TCRA were rearranged. Molecular analysis of the t(14;14) clearly demonstrated that in some tumors rearrangements of the IGH and TCRA genes are associated with interchromosomal exchanges that result in specific chromosome translocations that confer a proliferative advantage and predisposition to leukemic transformation. The implication of these rearrangements for normal and neoplastic T-cell development is discussed.
Jhanwar
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SC
Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
de Sostoa
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A
Doucette
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LA
Posnett
D N
DN
D015139:Blotting, Southern; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14; D003001:Cloning, Molecular; D015342:DNA Probes; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D015458:Leukemia, T-Cell / Q000235:genetics*; D008297:Male; D009693:Nucleic Acid Hybridization; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D014178:Translocation, Genetic
D015342:DNA Probes; D007143:Immunoglobulin Heavy Chains; D011948:Receptors, Antigen, T-Cell