Evidence for lectin-mediated adherence of S. saprophyticus and P. aeruginosa to polymers.
By hemagglutination tests surface lectins on S. saprophyticus strain S 1 with N-acetylgalactosamine (GalNac) and N-acetylglucosamine (GlcNac) specificity and on P. aeruginosa ATCC strain 27853 with N-acetylneuraminic acid (NANA) specificity could be demonstrated. To elucidate the role of bacterial surface lectins for the specific adhesion, polyether urethane discs were preincubated for 15 h at 4 degrees C in human serum or urine. Adhesion studies with S. saprophyticus S1 and P. aeruginosa ATCC 27853 onto precoated polymers revealed that microbial lectins may play a role in the colonization of prosthetic devices since lectin-blocking with competitive glycoconjugates significantly decreased bacterial adherence to the coated surfaces. Non-specific carbohydrates did not inhibit the adherence demonstrating specificity of this process.
Jansen
B
B
Institut für Medizinische Mikrobiologie, Universität zu Köln.
Beuth
J
J
Ko
H L
HL
D001422:Bacterial Adhesion / Q000187:drug effects; D001667:Binding, Competitive; D002241:Carbohydrates / Q000494:pharmacology; D006001:Glycoconjugates / Q000494:pharmacology; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D011140:Polyurethanes / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D013210:Staphylococcus / Q000378:metabolism*
D002241:Carbohydrates; D006001:Glycoconjugates; D037102:Lectins; D011140:Polyurethanes; C115016:polyetherurethane