title: Characterization of a Gla-containing protein from calcified human atherosclerotic plaques.
abstract: In this article we describe the isolation of a 4-carboxyglutamic acid (Gla)-containing protein from calcified human atherosclerotic plaques. The protein was extracted from pulverized calcified plaques by demineralization with ethylenediaminetetraacetate and was subsequently purified by anion-exchange fast protein and high-performance liquid chromatography by using ion-exchange and gel-filtration columns. The protein was designated as plaque Gla protein (PGP) and has an apparent mass of 23 kD as estimated from sodium dodecyl sulfate-polyacrylamide gel analysis. By determining the sequence of its first six amino acid residues, the protein was unequivocally demonstrated to be not related to any other known protein. Moreover, no immunological relationship (as tested by Western blot analysis) was found between PGP and other known Gla-containing proteins.
authors.affiliation: Department of Biochemistry, University of Limburg, Maastricht, The Netherlands.
authors.forename: B L
authors.initials: BL
authors.lastname: Gijsbers
authors.forename: L J
authors.initials: LJ
authors.lastname: van Haarlem
authors.forename: B A
authors.initials: BA
authors.lastname: Soute
authors.forename: R H
authors.initials: RH
authors.lastname: Ebberink
authors.forename: C
authors.initials: C
authors.lastname: Vermeer
mesh_terms: D015055:1-Carboxyglutamic Acid / Q000032:analysis*; D000596:Amino Acids / Q000032:analysis; D001011:Aorta / Q000737:chemistry*; D001161:Arteriosclerosis / Q000378:metabolism*; D015153:Blotting, Western; D002114:Calcinosis / Q000378:metabolism*; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004492:Edetic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D000596:Amino Acids; D011506:Proteins; D015055:1-Carboxyglutamic Acid; D004492:Edetic Acid