title: Antigen and antibody detection and update on the diagnosis of dengue.
abstract: The diagnosis of dengue is an imperfect science. By 1983 we had sensitive isolation systems including arthropods and arthropod cell cultures, specific-and group-reactive monoclonal antibodies, and a rapid and sensitive immunoassay for IgM and IgG. Progress in the past 7 years encompasses the use of monocyte lines for primary isolation, a more rapid plaque reduction neutralization test with BHK-21 cells, labeled RNA probes to detect dengue-specific nucleic acid, and improved ELISA technology with specific synthetic and engineered antigens.
authors.affiliation: Yale Arbovirus Research Unit, New Haven, CT 06510.
authors.forename: R E
authors.initials: RE
authors.lastname: Shope
mesh_terms: D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D003715:Dengue / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000639:trends; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral