title: Purification, characterization, and possible functions of small molecular weight GTP-binding proteins.
abstract: GTP-binding proteins with molecular weights of approximately 20,000 are designated small Mr G proteins. We purified four small Mr G proteins to near homogeneity from bovine brain crude membranes. Properties of these proteins, 20K G, 21K G, 22K G, and 24K G, were then assessed. The cDNA of 22K G and 24K G were cloned, and their nucleotide and amino acid sequences were determined. Three types of both C-terminal sequences and amino acid sequences were identified. We conclude that small Mr G proteins and large Mr G proteins are components of large intracellular regulatory and messenger systems with specific roles in cell function regulation.
authors.affiliation: Department of Biochemistry, Kobe University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Takai
authors.forename: A
authors.initials: A
authors.lastname: Kikuchi
authors.forename: M
authors.initials: M
authors.lastname: Kawata
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.forename: M
authors.initials: M
authors.lastname: Hoshijima
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D004247:DNA / Q000235:genetics; D019204:GTP-Binding Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008970:Molecular Weight; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism
chemical_list: D004247:DNA; D011065:Poly(ADP-ribose) Polymerases; D019204:GTP-Binding Proteins