Naturally occurring human IgG antibodies to intracellular and cytoskeletal components of human platelets.
Immunoblotting of platelets that have been subjected to SDS-PAGE has revealed that sera from normal individuals contain IgG which binds to many platelet components. This binding was seen with autologous and heterologous platelets using serum of males and of nulliparous females who had not received blood transfusions. Although binding patterns of different sera were not identical, almost all sera caused IgG binding to platelet components of 87-90 kD, 140 kD (identified as vinculin) and 220-240 kD (tentatively identified as talin and actin-binding protein). Purified IgG showed the same binding pattern as whole serum and F(ab')2 fragments retained their ability to bind to many components. The titre of IgG binding in serum was 1:50-1600 while that of alloantibodies to the PlA1 antigen was 1:3200. IgG binding components were not secreted when platelets were stimulated and were rarely associated with isolated membranes, but were located either in platelet cytoplasm or cytoskeletons. IgG binding was decreased by absorbing sera with lysed platelets or isolated cytoskeletons, but only slightly with intact platelets. Microaffinity purification of IgG which formed a major band on immunoblots showed that it was antibody with specificity for vinculin or its degradation products. These findings suggest that normal sera contain naturally occurring IgG antibodies with specificity for intracellular platelet antigens and that in some cases their titre approaches that of antibodies of pathological significance.
Pfueller
S L
SL
Department of Medicine, Monash University Medical School, Alfred Hospital, Prahran, Victoria, Australia.
Logan
D
D
Tran
T T
TT
Bilston
R A
RA
D000328:Adult; D000918:Antibody Specificity; D001323:Autoantibodies / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology* / Q000648:ultrastructure; D003598:Cytoskeletal Proteins / Q000276:immunology; D003599:Cytoskeleton / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000276:immunology*; D007425:Intracellular Membranes / Q000276:immunology*; D012016:Reference Values; D016596:Vinculin
D001323:Autoantibodies; D003598:Cytoskeletal Proteins; D007074:Immunoglobulin G; D016596:Vinculin