Removal of residual luteinizing hormone by the use of a specific receptor and antibody coupled to glass beads.
We describe here, a novel and effective use of a specific receptor for Luteinizing Hormone (LH) coupled to controlled pore-glass beads (CPG) and LH antibodies to glass beads (GB), in selective removal of residual LH from purified Follicle Stimulating Hormone (FSH) and Thyroid Stimulating Hormone (TSH). The LH receptor was prepared from bovine corpora lutea. LH antisera were raised in rabbits and purified. FSH and TSH preparations were purified by treatment with the LH receptor coupled to CPG-beads; and/or LH-antibody coupled to glass beads. This procedure avoided dilution of the FSH or TSH during purification.
Rathnam
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P
Cornell University Medical College, Department of Obstetrics & Gynecology, New York, N.Y. 10021.
Saxena
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EB
D000818:Animals; D000906:Antibodies; D002417:Cattle; D005640:Follicle Stimulating Hormone / Q000302:isolation & purification*; D005898:Glass; D006801:Humans; D007118:Immunoassay; D007986:Luteinizing Hormone / Q000276:immunology / Q000302:isolation & purification*; D008863:Microspheres; D011817:Rabbits; D011974:Receptors, LH / Q000378:metabolism*; D013972:Thyrotropin / Q000378:metabolism
D000906:Antibodies; D011974:Receptors, LH; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin