The effect of OP 2507, a stable analogue of prostacyclin, on Hep G2 exposed to hypoxia.
We developed a model for screening drugs to reduce ischemic liver damage using Hep G2, a hepatoblastoma cell line, and examined the effect of OP 2507, a stable analogue of prostacyclin, on hypoxic cell damage. Hypoxic exposure of the cells was done for 16 hr in an air-tight chamber which was placed inside an incubator and was purged with 5% CO2/95% N2. Adding OP 2507 (0.01-10 ng/ml) to the incubation medium during hypoxic exposure reduced mitochondrial damage estimated by MTT-reducing activity, while it failed to inhibit lactate accumulation in the medium. OP 2507 seems to be a good candidate for improving the preservation of liver allografts.
Irita
K
K
Department of Anesthesiology and Critical Care Medicine, Kyushu University School of Medicine, Fukuoka.
Sakai
H
H
Isobe
H
H
Yamakawa
M
M
Nawata
H
H
Yoshitake
J A
JA
D000818:Animals; D002460:Cell Line; D004353:Drug Evaluation, Preclinical; D011464:Epoprostenol / Q000031:analogs & derivatives* / Q000494:pharmacology; D000860:Hypoxia; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D008114:Liver Neoplasms, Experimental; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009926:Organ Preservation; D051381:Rats
D007773:Lactates; C049453:OP 2507; D011464:Epoprostenol; D007770:L-Lactate Dehydrogenase