[Synthesis of oligoribonucleotides by the N-phosphonate method using alkali-labile 2'-o-protective groups. II. Various aspects of using 2'-o-benzoyl and anisole protective groups].
The N-acyl, 5'-O-trityl (MeOTr, (MeO)2Tr, Me3Tr), 2'-O-benzoyl (and anisole) nucleosides were prepared by selective aroylation of N,5'-protected nucleosides. By means of the reverse-phase microcolumn liquid chromatography it was shown that the rate of the aryl 2'----3'-isomerisation is lower in case of 2'-anisoylnucleosides and depends on structure of the 5'-O-protecting group. The prepared synthons were used for the manual H-phosphonate solid-phase synthesis of oligoribonucleotides (6-10-mers).
Rozners
E Z
EZ
Rekis
A Kh
AKh
Kumpin'sh
V Kh
VKh
Bizdena
E O
EO
D001483:Base Sequence; D002848:Chromatography, DEAE-Cellulose; D002851:Chromatography, High Pressure Liquid; D007700:Kinetics; D008969:Molecular Sequence Data; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D063065:Organophosphonates / Q000737:chemistry
D009843:Oligoribonucleotides; D063065:Organophosphonates
Sintez oligoribonukleotidov N-fosfonatnym metodom s izpol'zovaniem shchelochnolabil'nykh 2'-o-zashchitnykh grupp. II. Nekotorye aspekty izpol'zovaniia 2'-o-benzoil'noĭ i anizoilnoĭ zashchitnykh grupp.