Cytokine measurements in body fluids.
Bioassays and immunoassays for cytokines are now widely available for use in clinical laboratories which may have little or no expertise in cytokine biology. Whilst this facilitates the accumulation of data concerning cytokine levels in body fluids in disease, it is based on the assumption that such assays can be used for this purpose. In many cases, the presence of complex interfering factors in plasma and other body fluids require that assays should be subjected to detailed assay validation before confidence can be placed on the results. It is the purpose of this report to outline the potential problems with cytokine assays and the criteria that should be applied before making measurements in biological fluids.
Whicher
J
J
Department of Chemical Pathology and Immunology, University of Leeds, Old Medical School, United Kingdom.
Ingham
E
E
D000818:Animals; D001681:Biological Assay; D001826:Body Fluids / Q000737:chemistry*; D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis* / Q000037:antagonists & inhibitors; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006801:Humans; D007118:Immunoassay; D051379:Mice; D011237:Predictive Value of Tests; D012015:Reference Standards
D016207:Cytokines