Neuronal potentialities of cells in the optic nerve of the chicken embryo are revealed in culture.
Neuronal potentialities in neuroepithelial cells of the chicken embryonic optic nerve were studied in culture by using neurofilament antibodies as neuronal markers. Embryonic day-4 and -5 (E4 and E5) optic stalks were explanted in vitro. Within the first few days of culture, numerous morphologically identifiable neurons extending long neurites developed. These neurons and their processes were specifically labeled with neurofilament antibodies. Similar results were obtained by explanting only the medial portion of E7 optic stalks away from possibly contaminating cerebral or retinal tissue. To determine whether neuronal potentialities persisted at later embryonic stages, cultures of dissociated optic stalks were established at E11, E15, and E18. Neurons labeled with the various neurofilament antibodies appeared in all cultures of E11 and E15 optic stalks. However, typical neurons could not be recognized in cultures of E18 optic nerves. These results indicate that cells with neuronal potentialities are present in the embryonic optic nerve from early stages of development and persist until at least E15. Since the adult optic nerve is devoid of nerve cell bodies, our observations are consistent with the hypothesis that axons of retinal ganglion cells, which course through the optic stalk, repress neuronal potentialities within a subpopulation of precursor cells during normal development.
Giess
M C
MC
Centre National de la Recherche Scientifique, Université Paul Sabatier, Toulouse, France.
Cochard
P
P
Duprat
A M
AM
D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000166:cytology / Q000196:embryology; D002642:Chick Embryo; D003599:Cytoskeleton / Q000648:ultrastructure*; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D007382:Intermediate Filaments / Q000648:ultrastructure*; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D009900:Optic Nerve / Q000166:cytology / Q000196:embryology*; D009924:Organ Culture Techniques; D013861:Thiocyanates
D000911:Antibodies, Monoclonal; D005452:Fluoresceins; D005456:Fluorescent Dyes; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate