The 70 kilodalton iron regulated protein of Neisseria meningitidis is not the human transferrin receptor.
Neisseria meningitidis is able to chelate iron from human transferrin (HTF), the main sequestrator of extracellular iron in vivo. Previous workers have reported that a ca. 70 kilodalton (kDa) iron regulated outer membrane protein (FeRP-70) is a highly specific receptor for HTF. We have examined the interaction between the iron regulated outer membrane proteins (OMP's) and HTF, using HTF and rabbit anti HTF, as well as gold labelled HTF (Au-HTF) to blot OMP's of various serogroups and serotypes of N. meningitidis. Also, we used monospecific rabbit anti FeRP-70 in competitive experiments to determine the role of FeRP-70 in HTF-binding. Single proteins (molecular weights range ca. 60 to ca. 90 kDa) were identified in the OMP's from each strain which reacted with HTF. HTF failed to block the reaction between FeRP-70 and the OMP's, conversely anti FeRP-70 failed to block the HTF-binding reaction. We believe that the 70 kDa iron regulated protein of N. meningitidis is not a human transferrin receptor.
Ala'Aldeen
D A
DA
Microbial Pathogenicity Research Group, MRC Clinical Research Centre, Harrow, Middlesex, U.K.
Davies
H A
HA
Wall
R A
RA
Borriello
S P
SP
D000293:Adolescent; D000328:Adult; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D001667:Binding, Competitive; D015153:Blotting, Western; D002648:Child; D006801:Humans; D007223:Infant; D007501:Iron / Q000378:metabolism*; D008875:Middle Aged; D008970:Molecular Weight; D009345:Neisseria meningitidis / Q000378:metabolism*; D011990:Receptors, Transferrin / Q000378:metabolism*; D014168:Transferrin / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; D011990:Receptors, Transferrin; D014168:Transferrin; D007501:Iron