[Affinity chromatographic purification of streptokinase with monoclonal antibodies].
Monoclonal antibodies against streptokinase as ligands were coupled to different matrices. Both CNBr-activated Sepharose and macroporous bead cellulose activated by carbonochloridate revealed as suitable for purification of streptokinase by affinity chromatography. Immunoadsorbents with a higher concentration of the coupled ligand were more effective than those with a lower. For streptokinase optimal conditions of binding and elution without negative influence on structure and activity were ascertained. A buffer with slight alkaline pH was successful for desorption. Using this method it was possible to obtain pure streptokinase from several streptokinase containing media.
Andreas
E M
EM
Bereich Tierphysiologie und Immunobiologie, Karl-Marx-Universität Leipzig.
D000911:Antibodies, Monoclonal / Q000276:immunology; D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D007158:Immunologic Techniques; D008970:Molecular Weight; D013291:Streptococcus / Q000201:enzymology; D013300:Streptokinase / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D013300:Streptokinase
Affinitätschromatographische Reinigung von Streptokinase mittels monoklonaler Antikörper.