Induction of umu gene expression in Salmonella typhimurium TA1535/pSK1002 by dimethyl sulfoxide (DMSO).
The genotoxicity of dimethyl sulfoxide (DMSO) was demonstrated by the umu test using Salmonella typhimurium TA1535/pSK1002 carrying the umuC-lacZ fusion gene. The level of beta-galactosidase activity which shows umu gene expression in the test system was dependent on the concentration of DMSO in the culture medium. The maximum beta-galactosidase activity was approximately 3.5 times as high as the background level with 10% of DMSO in the culture medium. The lowest concentration of DMSO required for a response of over twice the background level was approximately 5%. Four structurally related chemicals (acetone, di-n-butylsulfoxide, dimethylsulfide, methylphenylsulfoxide) did not show umu gene expression at their non-toxic doses.
Nakamura
S
S
Osaka Prefectural Institute of Public Health, Japan.
Oda
Y
Y
Ugawa
M
M
D003001:Cloning, Molecular; D004121:Dimethyl Sulfoxide / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects*; D005798:Genes, Bacterial / Q000187:drug effects*; D009153:Mutagens; D013014:SOS Response, Genetics / Q000187:drug effects; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D013329:Structure-Activity Relationship; D001616:beta-Galactosidase / Q000235:genetics
D009153:Mutagens; D001616:beta-Galactosidase; D004121:Dimethyl Sulfoxide