A simple serum galactosyltransferase assay method suitable for routine use.
UDP-Galactose: N-acetylglucosaminyl glycoprotein beta 1-4 galactosyltransferase (GT) catalyzes the transfer of galactose to N-acetylglucosamine from UDP-[3H]Gal. The uncharged reaction product (tritiated N-acetyllactosamine) is separated from the unreacted UDP-[3H]Gal by ion-exchange chromatography. The major advantage of this method is its rapidity compared to other isotopic techniques.
Sichel
F
F
Laboratoire de Biochimie Clinique et Expérimentale, Centre F. Baclesse Caen, France.
Malas
J P
JP
Gauduchon
P
P
Aubert
M
M
Bar
E
E
Le Talaër
J Y
JY
D002852:Chromatography, Ion Exchange; D005260:Female; D006801:Humans; D007788:Lactose Synthase / Q000097:blood*; D008297:Male; D009237:N-Acetyllactosamine Synthase / Q000097:blood*; D012016:Reference Values
D007788:Lactose Synthase; D009237:N-Acetyllactosamine Synthase