title: Murine monoclonal antibody MB-2D10 recognizes Rh-related glycoproteins in the human red cell membrane.
abstract: The human red cell membrane components reacting with monoclonal antibody MB-2D10 were examined by immunoblotting. The antibody bound to a diffusely staining band extending from Mr 30,000 up to the high-molecular-weight region of the gel in normal membranes and in Rhnull U + membranes, but not in Rhnull U - membranes. Treatment of normal red cells with an endoglycosidase F-containing preparation destroyed the epitope recognized by MB-2D10. The reactivity of the antibody with purified preparations of Rh-related glycoproteins D30 polypeptide, D50 polypeptide, R6A32 polypeptide, and R6A45 polypeptide was also examined. Only the purified R6A45 and D50 components reacted with MB-2D10. These results show that MB-2D10 recognizes a carbohydrate-dependent epitope on the R6A45 and D50 group of Rh-related polypeptides. The results also suggest the possibility that the U antigen arises from interaction between glycophorin B and the Rh-related components D50 and R6A45.
authors.affiliation: Blood Group Reference Laboratory, South Western Regional Blood Transfusion Centre, Southmead, Bristol, UK.
authors.forename: G
authors.initials: G
authors.lastname: Mallinson
authors.forename: D J
authors.initials: DJ
authors.lastname: Anstee
authors.forename: N D
authors.initials: ND
authors.lastname: Avent
authors.forename: K
authors.initials: K
authors.lastname: Ridgwell
authors.forename: M J
authors.initials: MJ
authors.lastname: Tanner
authors.forename: G L
authors.initials: GL
authors.lastname: Daniels
authors.forename: P
authors.initials: P
authors.lastname: Tippett
authors.forename: A E
authors.initials: AE
authors.lastname: von dem Borne
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004910:Erythrocyte Membrane / Q000032:analysis*; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D012204:Rh-Hr Blood-Group System
chemical_list: D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D012204:Rh-Hr Blood-Group System