[Partial purification of galactocerebroside sulfotransferase in the rat brain].
We describe a method of partial purification of rat brain 3'-phosphoadenosine-5'-phosphosulfate galactocerebroside sulfotransferase (EC 2.8.2.11, CST). The first steps consist of a Triton X-100 extraction of the enzyme from delipidated microsomes and the fractionation of this extract by precipitation with ammonium sulfate, followed by successive chromatography on hydrophobic gel (phenyl sepharose), heparin, gel filtration (Trisacryl GF2000), hydroxy-apatite and cation exchange resin (SP trisacryl). The denaturation of the enzyme and its instability account for the low enrichment achieved in terms of specific activity, but an evident simplification of the electrophoretic pattern was obtained.
Fressinaud
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C
Centre de Neurochimie, C.N.R.S., Strasbourg.
Sarliève
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LL
Labourdette
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D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000648:ultrastructure; D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D008861:Microsomes / Q000201:enzymology; D051381:Rats; D015238:Sulfotransferases; D013466:Sulfurtransferases / Q000302:isolation & purification*
D013466:Sulfurtransferases; D015238:Sulfotransferases; C020556:galactosylceramide sulfotransferase
Purification partielle de la galactocérébroside sulfotransférase de cerveau de rat.