title: Can storage of thawed cryoprecipitate be extended to more than six hours?
abstract: Fresh-frozen plasma (FFP) and cryoprecipitate both contain Factors I and VIII, however thawed FFP may be stored at 1-6 degrees C for 24 hours, but thawed cryoprecipitate may only be stored at 1-6 degrees C for six hours when used for Factor VIII content. To determine whether it would be safe and effective to extend the storage period of thawed cryoprecipitate from 6 to 24 hours, Factor VIII (and fibrinogen) levels were measured in 25 units of cryoprecipitates immediately on thawing and at 6 hours and 24 hours thereafter. The Factor VIII activity level decreased to 86% of the original activity level within 6 hours, but the drop between 6 and 24 hours was relatively small. Eighty percent of the original activity was still present at 24 hours after thawing. The fibrinogen level decreased to 87% of the original level within 6 hours but remained stable between 6 and 24 hours. Additional testing showed that fibrinogen levels remained stable between 6 and 74 hours. These data suggested that the storage of thawed cryoprecipitate might be extended to 24 hours when this blood product is used for Factor VIII content and to 74 hours when it is used for fibrinogen content. Furthermore, the lack of prohibition on the use of cryoprecipitate that has been thawed for more than six hours and stored at 4 degrees C for its fibrinogen content seems reasonable.
authors.affiliation: Coagulation Sections of Pathology, Los Angeles County, University of Southern California Medical Center.
authors.forename: S
authors.initials: S
authors.lastname: Saxena
authors.forename: V
authors.initials: V
authors.lastname: Odono
authors.forename: R B
authors.initials: RB
authors.lastname: Francis
authors.forename: G L
authors.initials: GL
authors.lastname: Endahl
authors.forename: I A
authors.initials: IA
authors.lastname: Shulman
mesh_terms: D001793:Blood Preservation; D015925:Cryopreservation; D005169:Factor VIII / Q000032:analysis*; D005340:Fibrinogen / Q000032:analysis*; D006801:Humans; D010949:Plasma / Q000032:analysis*; D013997:Time Factors
chemical_list: C026912:cryoprecipitate coagulum; D005169:Factor VIII; D005340:Fibrinogen