pp60c-src protein kinase activity in human gastric carcinomas.
We examined pp60c-src protein kinase activity in human gastric carcinoma cell lines and gastric carcinoma tissues as well as normal mucosa. pp60c-src kinase activity was detected in all 5 carcinoma cell lines at various levels. Of 16 gastric carcinoma tissues, 8 showed higher pp60c-src kinase activity in tumor tissues than in corresponding normal mucosa. However, the levels of expression of pp60c-src detected by Western blotting were not always consistent with the activities of pp60c-src protein kinase. These findings suggest that the increase in pp60c-src protein kinase activity might be brought about by post-translational changes.
Takekura
N
N
First Department of Pathology, Hiroshima University School of Medicine, Japan.
Yasui
W
W
Yoshida
K
K
Tsujino
T
T
Nakayama
H
H
Kameda
T
T
Yokozaki
H
H
Nishimura
Y
Y
Ito
H
H
Tahara
E
E
D015152:Blotting, Northern; D015153:Blotting, Western; D002460:Cell Line; D015870:Gene Expression; D006801:Humans; D009693:Nucleic Acid Hybridization; D015688:Oncogene Protein pp60(v-src) / Q000235:genetics / Q000378:metabolism*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000235:genetics; D014407:Tumor Cells, Cultured / Q000201:enzymology
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011494:Protein Kinases; D015688:Oncogene Protein pp60(v-src)