Long-term preservation of blood samples for diagnosis of Trypanosoma cruzi infection.
Feasability and suitability for field research of a whole-blood preservation method was evaluated through the screening of anti-Trypanosoma cruzi antibodies in 1209 samples under different conditions. Antibody reactivity of paired samples from preserved capillary blood (CBP) and sera from venous blood (VBS) were studied by specific techniques. Over 96% concordance was found on indoor studies carried out with samples without storage or after 15 or 30 days preservation of CBP at 37 degrees C and VBS at -20 degrees C. Outdoor studies performed at field conditions, achieved a 92.1% concordance.
Pérez
A C
AC
Instituto Nacional de Diagnóstico e Investigación de la Enfermedad de Chagas Dr. Mario Fatala Chabén, Secretaría de Salud Ministerio de Salud y Acción Social, Argentina.
Cura
E
E
Subías
E
E
Lansetti
J C
JC
Segura
E L
EL
D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D001793:Blood Preservation / Q000379:methods*; D014355:Chagas Disease / Q000175:diagnosis*; D005069:Evaluation Studies as Topic; D006801:Humans; D014349:Trypanosoma cruzi / Q000276:immunology*
D000913:Antibodies, Protozoan