title: [A study on LH- and FSH-RIA kits by immunoradiometric assay].
abstract: An evaluation of LH- and FSH-RIA BEAD kits based on IRMA was carried out. The results obtained with the methods characterized by the use of monoclonal antibodies, i.e., one is linked to solid phase, and another is isotopically labeled, were compared with those determined by the Daiichi LH- and FSH-kits. Intra- and inter-assay precision, recovery, linearity, and specificity of both methods were favorable without exceptions. The cross reactivity of the LH kit to 5,000 mIU/ml hCG revealed within the range of less than 3 mIU/ml. Significant correlations were observed between the results derived from conventional Daiichi LH- and FSH-kits. The results from the conventional kits exhibited 30 to 40% of those from the Daiichi kits, considered to be mainly due to the difference in standard calibrations used. Among the individuals within the normal menstrual cycle, the serum LH- and FSH-levels determined by the present kits gave a typical pattern with a peak in the preovulatory phase. On the other hand, the LH- and FSH-values of individuals in normal pregnancy revealed strikingly decreased in comparison with those of non-pregnant women.
authors.affiliation: Department of Radiology, Shinshu University Hospital, Matsumoto-shi, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kobayashi
authors.forename: R
authors.initials: R
authors.lastname: Sakai
authors.forename: S
authors.initials: S
authors.lastname: Satoh
authors.forename: M
authors.initials: M
authors.lastname: Kanai
mesh_terms: D000328:Adult; D005069:Evaluation Studies as Topic; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood*; D005498:Follicular Phase / Q000502:physiology; D006801:Humans; D007986:Luteinizing Hormone / Q000097:blood*; D011247:Pregnancy; D011863:Radioimmunoassay; D011933:Reagent Kits, Diagnostic
chemical_list: D011933:Reagent Kits, Diagnostic; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone