title: Glycine-induced cryotransformation of plasmids into Bacillus anthracis.
abstract: Different cloning vectors (pC194, pBC16, pUB110, pBD10, pBD8, pAM beta 1) and Bacillus anthracis plasmid pX02 were introduced into B. anthracis by a transformation method. To induce an artificial competence state for uptake of isolated plasmid DNA, the cultures were treated with glycine, to reduce cross-linking of peptidoglycan, followed by freezing and thawing. The procedure is extremely rapid and relatively efficient (maximum transformation efficiency about 10(3) c.f.u. per micrograms DNA) and allows different cloning vectors with molecular masses ranging from 1.8 to 17.7 MDa to be introduced into B. anthracis.
authors.affiliation: Laboratory of Isotope Analysis, All-Union Antiplague Research Institute Microbe, Saratov, USSR.
authors.forename: A S
authors.initials: AS
authors.lastname: Stepanov
authors.forename: O B
authors.initials: OB
authors.lastname: Puzanova
authors.lastname: Dityatkin SYa
authors.forename: O G
authors.initials: OG
authors.lastname: Loginova
authors.forename: B N
authors.initials: BN
authors.lastname: Ilyashenko
mesh_terms: D001408:Bacillus anthracis / Q000235:genetics*; D003001:Cloning, Molecular; D003470:Culture Media; D005615:Freezing; D005822:Genetic Vectors; D005998:Glycine / Q000494:pharmacology*; D008274:Magnesium / Q000494:pharmacology; D010957:Plasmids; D011092:Polyethylene Glycols / Q000494:pharmacology; D014169:Transformation, Bacterial
chemical_list: D003470:Culture Media; D011092:Polyethylene Glycols; D008274:Magnesium; D005998:Glycine