title: Soluble calcium-binding proteins: parvalbumins and calmodulin from eel skeletal muscle.
abstract: 1. Eel skeletal muscle contains three parvalbumin isoforms. The overall parvalbumin concentration in the muscle is 0.5 mmol kg-1 wet weight. 2. Calmodulin (0.1 mumol kg-1 wet weight) was purified by extraction with ethylenediamine tetraacetate-containing buffer, fractionation with trichloroacetic acid and separation by ion exchange chromatography on DEAE-cellulose and by molecular sieving on Ultrogel AcA 54. 3. Troponin-C-free calmodulin was obtained by fast protein liquid chromatography on a Mono Q column.
authors.affiliation: Institut de Chimie (B6) au Sart Tilman, Université de Liège, Belgium.
authors.forename: I
authors.initials: I
authors.lastname: Dubois
authors.forename: C
authors.initials: C
authors.lastname: Gerday
mesh_terms: D000811:Anguilla / Q000378:metabolism*; D000818:Animals; D002135:Calcium-Binding Proteins / Q000302:isolation & purification*; D002147:Calmodulin / Q000302:isolation & purification*; D008239:Lysine / Q000031:analogs & derivatives / Q000302:isolation & purification; D009124:Muscle Proteins / Q000302:isolation & purification*; D009132:Muscles / Q000032:analysis*; D010320:Parvalbumins / Q000302:isolation & purification*; D012995:Solubility; D014336:Troponin / Q000302:isolation & purification; D019209:Troponin C
chemical_list: D002135:Calcium-Binding Proteins; D002147:Calmodulin; D009124:Muscle Proteins; D010320:Parvalbumins; D014336:Troponin; D019209:Troponin C; C003712:trimethyllysine; D008239:Lysine