Monoclonality in B-lymphoproliferative disorders detected at the DNA level.
A new method was developed for detection of monoclonality at the DNA level in B-lymphoproliferative disease using the polymerase chain reaction and consensus primers for the V and J regions of the immunoglobulin gene. Monoclonality was detected in DNA from 15 of 15 clonal B-lymphoblastoid cell lines and from 19 of 23 cases of B-lymphocyte neoplasia, but not from any of 16 normal T-lymphocyte clones, 9 cases of T-lymphocyte neoplasia, 20 samples of polyclonal peripheral blood lymphocytes, and 8 cases of reactive lymphadenopathy. This method for detection of monoclonality is likely to be of routine value in diagnosis owing to its simplicity, speed, and versatility.
Trainor
K J
KJ
Department of Hematology, Flinders Medical Center, Bedford Park, Australia.
Brisco
M J
MJ
Story
C J
CJ
Morley
A A
AA
D001402:B-Lymphocytes / Q000473:pathology; D001483:Base Sequence; D004247:DNA / Q000032:analysis / Q000235:genetics*; D015342:DNA Probes; D004587:Electrophoresis, Agar Gel; D005784:Gene Amplification / Q000235:genetics; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D008232:Lymphoproliferative Disorders / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction
D015342:DNA Probes; D007143:Immunoglobulin Heavy Chains; D004247:DNA