Inhibition of human and rabbit platelet activation by Ketotifen.
The effects of Ketotifen and disodium cromoglycate were investigated on human and rabbit platelet activation. Ketotifen inhibited dose-dependently human and rabbit platelet aggregation. The paf-acether pathway was the most markedly influenced by Ketotifen in human and rabbit platelets (IC50 = 38.8 +/- 7.7 microM and 7.2 +/- 4.5 microM respectively) as compared to adenosine diphosphate (IC50 greater than 100 microM and 79 +/- 19 microM) and to arachidonic acid (IC50 greater than 100 microM and 98 +/- 28 microM). Similar concentrations of Ketotifen inhibited the ATP release from human platelets induced by paf-acether. Disodium cromoglycate up to 5 x 10(-4) M did not inhibit platelet aggregation induced by paf-acether, adenosine diphosphate and arachidonic acid.
Devillier
P
P
Service de Pneumologie, Hôpital Cochin, Paris, France.
Arnoux
B
B
Lalau Keraly
C
C
Landes
A
A
Marsac
J
J
Benveniste
J
J
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000201:enzymology; D004205:Cromolyn Sodium / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007665:Ketotifen / Q000494:pharmacology*; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors*; D015539:Platelet Activation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011817:Rabbits
D010972:Platelet Activating Factor; D010975:Platelet Aggregation Inhibitors; D000255:Adenosine Triphosphate; D004205:Cromolyn Sodium; D007665:Ketotifen