title: Sensitive detection of Mycoplasma pulmonis by using the polymerase chain reaction.
abstract: Detection of Mycoplasma pulmonis was examined by using the polymerase chain reaction (PCR) for amplifying a specific DNA sequence. In gel electrophoresis which was conducted to detect the amplified products, only 1 pg of M. pulmonis DNA could be detected following 30 cycles of amplification, while no amplified product was detected even from 1 microgram of M. arthritidis or M. neurolyticum DNA. Furthermore, 10 colony-forming units of M. pulmonis could be detected by direct amplification from the mycoplasma suspension. These results suggest the usefulness of the PCR as a highly sensitive, specific, and rapid method for direct detection of M. pulmonis.
authors.affiliation: Department of Laboratory Animal Science, School of Hygienic Sciences, Kitasato University, Sagamihara-shi, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kunita
authors.forename: E
authors.initials: E
authors.lastname: Terada
authors.forename: K
authors.initials: K
authors.lastname: Goto
authors.forename: N
authors.initials: N
authors.lastname: Kagiyama
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004259:DNA-Directed DNA Polymerase; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D009177:Mycoplasma pneumoniae / Q000302:isolation & purification*; D009841:Oligonucleotides; D016133:Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial; D009841:Oligonucleotides; D004259:DNA-Directed DNA Polymerase