title: Hepatocyte regeneration after partial hepatectomy occurs even under severely thrombocytopenic conditions in the rat.
abstract: In order to investigate the role of blood platelets in regenerative proliferation of hepatocytes in vivo, partial hepatectomy was carried out under conditions of severe thrombocytopenia achieved by X-irradiation and the time-dependent degree of hepatocyte regeneration was measured as labeling index (LI) after 3H-thymidine injection. LI values reached a maximum 36 h after hepatectomy performed 7 days subsequent to a whole-body irradiation of 7.5 Gy, although the maximum rate was found to be only about one-third of that found in unirradiated control rats. At this time point, the number of peripheral blood platelets was decreased to about 2% of that of unirradiated controls and bone marrow megakaryocytes were virtually undetectable. A decrease of LI, similar to that found for the whole-body irradiation in degree, was also observed after local irradiation of the liver with the same dose but without an apparent decrease in platelets. When whole-body irradiation was performed with the liver shielded, under which conditions the numbers of blood platelets decreased to about 8% of the control levels, almost no decrease in LI was found. The results thus suggest that the observed inhibition of hepatocyte regeneration was directly due to liver irradiation and that depletion of blood platelets itself does not interfere with the regeneration process, providing evidence against a dominant role of the platelets in the regeneration as proposed by other authors.
authors.affiliation: Department of Experimental Pathology, Cancer Institute, Tokyo.
authors.forename: Y
authors.initials: Y
authors.lastname: Kuwashima
authors.forename: K
authors.initials: K
authors.lastname: Aoki
authors.forename: K
authors.initials: K
authors.lastname: Kohyama
authors.forename: T
authors.initials: T
authors.lastname: Ishikawa
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D004906:Erythrocyte Count; D006498:Hepatectomy; D008099:Liver / Q000528:radiation effects; D008115:Liver Regeneration; D008297:Male; D010976:Platelet Count / Q000528:radiation effects; D051381:Rats; D011919:Rats, Inbred Strains; D013921:Thrombocytopenia / Q000503:physiopathology*