The upstream region of the IPNS gene determines expression during secondary metabolism in Aspergillus nidulans.
We have constructed a translational fusion between the isopenicillin-N-synthetase-encoding gene (IPNS) of Aspergillus nidulans and the lacZ gene of Escherichia coli. Recombinant strains carrying a single copy of the fusion integrated at the IPNS locus produced beta-galactosidase (beta Gal) during secondary metabolism. Integration of the fusion at the argB locus results in a situation in which the only 5'-flanking sequences of the IPNS gene upstream from the chimeric fused gene are those included in the transforming plasmid. Such a strain still expresses beta Gal activity during secondary metabolism, showing that a DNA fragment including sequences of the IPNS gene from nt -2000 to +35 (relative to the translation start codon) still contains sufficient information to drive expression of the fusion gene during secondary metabolism.
Gómez-Pardo
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Centro de Investigaciones Biológicas, Velázquez, Madrid, Spain.
Peñalva
M A
MA
D001233:Aspergillus nidulans / Q000235:genetics* / Q000378:metabolism; D015139:Blotting, Southern; D015153:Blotting, Western; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D005800:Genes, Fungal; D007700:Kinetics; D008970:Molecular Weight; D010088:Oxidoreductases / Q000235:genetics* / Q000302:isolation & purification; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism; D015183:Restriction Mapping; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D004271:DNA, Fungal; D011993:Recombinant Fusion Proteins; D010088:Oxidoreductases; C038370:isopenicillin N synthetase; D001616:beta-Galactosidase