S/RV Cri-ba, a hairless mouse strain sensitive to skin tumorigenesis by suboptimal doses of 7,12-dimethylbenz[a]anthracene, initiation-promotion and two stage promotion protocols.
Susceptibility of hairless inbred S/RV Cri-ba or Bare mice to skin tumor development with suboptimal doses of 7,12-dimethylbenz[a]anthracene (DMBA), DMBA-TPA two stage protocol and two stage promotion using 12-O-tetradecanoylphorbol-13-acetate (TPA) as sub-stage 1 promoter and mezerein (MEZ) or phorbol retinoate acetate (PRA) as substage 2 promoter was determined. A single application of 40 or 20 nmol DMBA induced 4-5 papillomas per mouse 40 weeks after initiation while no tumors appeared after similar treatment with 10 or 4 nmol DMBA. Dose response studies for DMBA initiation revealed that 10 nmol DMBA dose saturated the sites for initiation in the resting epidermis. In two stage promotion experiments, MEZ was found to be a potent stage 2 promoter, while PRA acted as a weak complete promoter.
Bhisey
R A
RA
Genotoxicity Unit, Cancer Research Institute, Bombay, India.
Veturkar
P L
PL
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D015197:Carcinogenicity Tests; D003043:Cocarcinogenesis; D004224:Diterpenes; D004305:Dose-Response Relationship, Drug; D005260:Female; D051379:Mice; D008812:Mice, Hairless; D010212:Papilloma / Q000139:chemically induced* / Q000473:pathology; D010703:Phorbol Esters / Q000633:toxicity; D012878:Skin Neoplasms / Q000139:chemically induced* / Q000473:pathology; D013729:Terpenes / Q000633:toxicity; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity
D004224:Diterpenes; D010703:Phorbol Esters; D013729:Terpenes; C011309:mezerein; D015127:9,10-Dimethyl-1,2-benzanthracene; C032974:12-O-retinoylphorbol-13-acetate; D013755:Tetradecanoylphorbol Acetate