title: Rabbit major histocompatibility complex. IV. Expression of major histocompatibility complex class II genes.
abstract: The rabbit MHC class II DP, DQ, and DR alpha and beta chain genes were transfected into murine B lymphoma cells. The transfected cells expressed R-DQ and R-DR molecules on the cell surface but they did not express the R-DP genes either on the cell surface or at the level of mRNA. Northern blot analyses showed that the R-DP genes were expressed, albeit at low levels, in rabbit spleen. Similar analyses showed that the R-DQ and R-DR genes were expressed at high levels in rabbit spleen. A new monoclonal anti-rabbit class II antibody, RDR34, has been developed and shown to react with the R-DR transfected cells and not with the R-DQ transfected cells. The previously described monoclonal anti-rabbit class II antibody, 2C4, reacted with the R-DQ transfected cells and not with the R-DR transfected cells. Thus, 2C4 and RDR34 MAb's are specific for the R-DQ and R-DR molecules, respectively. Each of the antibodies reacted with approximately 50% of rabbit spleen cells as shown by immunofluorescent antibody studies.
authors.affiliation: Department of Microbiology, Stritch School of Medicine, Loyola University of Chicago, Maywood, Illinois 60153.
authors.forename: H
authors.initials: H
authors.lastname: Spieker-Polet
authors.forename: N
authors.initials: N
authors.lastname: Sittisombut
authors.forename: P C
authors.initials: PC
authors.lastname: Yam
authors.forename: K L
authors.initials: KL
authors.lastname: Knight
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D015870:Gene Expression; D005802:Genes, MHC Class II; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis / Q000235:genetics; D011817:Rabbits / Q000235:genetics* / Q000276:immunology; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II