title: Comparison of the retention in chicks and mice of 59Fe given orally as chloride, ethylene diamine tetra-acetate or nitrilotriacetate and in chicks given diets composed of conventional ingredients or semi-purified nutrients.
abstract: In experiment 1 chicks and growing mice given diets containing 400 mg Fe kg-1, added as chloride, ethylene diamine tetra-acetate or nitrilotriacetate (NTA), were dosed orally with 59Fe in the form present in the diet and the quantities of 59Fe in liver, spleen, blood, remaining carcase and whole blood were calculated as concentration in fresh tissue and as a percentage of the dose. Retention as concentration of 59Fe in fresh tissue was significantly greater in mice than chicks for the spleen and blood (P less than 0.001) and greater in chicks than mice for the liver (P less than 0.05) and carcase (P less than 0.01). Overall differences from the form of iron were significant for the spleen, carcase and whole body (P less than 0.01). These were given primarily by high values for chloride and low for NTA. Percentage retention values gave a similar overall picture to that for concentration. In experiment 2, chicks given diets based on conventional ingredients or semi-purified nutrient with supplements providing 400 mg Fe kg-1 were dosed orally with 59Fe and retention was determined in liver, spleen, blood, remaining carcase and whole body, as in experiment 1. Retention as concentration was significantly greater for the semi-purified nutrient diet than the conventional one in all tissues, at P less than 0.01 in liver and P less than 0.001 in other tissues. There were no significant overall effects from the form of iron added as the supplement.
authors.affiliation: Royal Veterinary College, Boltons Park, Potters Bar, Hertfordshire.
authors.forename: M J
authors.initials: MJ
authors.lastname: Leighton
authors.forename: A
authors.initials: A
authors.lastname: Bhabuta
authors.forename: R
authors.initials: R
authors.lastname: Hill
mesh_terms: D000284:Administration, Oral; D000821:Animal Feed; D000818:Animals; D002645:Chickens / Q000378:metabolism*; D002712:Chlorides; D004032:Diet; D004492:Edetic Acid / Q000008:administration & dosage / Q000493:pharmacokinetics; D005290:Ferric Compounds / Q000008:administration & dosage / Q000493:pharmacokinetics; D007501:Iron / Q000097:blood / Q000493:pharmacokinetics*; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice / Q000378:metabolism*; D009571:Nitrilotriacetic Acid / Q000008:administration & dosage / Q000493:pharmacokinetics; D013154:Spleen / Q000378:metabolism
chemical_list: D002712:Chlorides; D005290:Ferric Compounds; D004492:Edetic Acid; D007501:Iron; D009571:Nitrilotriacetic Acid; C024555:ferric chloride