The frequency of multiple recombination events occurring at the human Ig kappa L chain locus.
Products of Ig kappa L chain gene rearrangement in a variety of human B cell samples were investigated by sequential Southern blot hybridization analysis. By application of four region-specific probes (C kappa, J kappa, U' kappa and kappa de) a complete spectrum of kappa rearrangements, including both predicted and novel products, were detected. Nearly 30% of the products detected reflect multiple recombination of the kappa locus. The kappa-deleting element was responsible for 70% of the multiple rearrangements that were detected. Interestingly, eight kappa-expressing samples exhibited rearrangement of the kappa-deleting element. The remaining multiple recombination products were characteristic of double V kappa-J kappa rearrangement. This frequency reveals that secondary V-J rearrangement may significantly contribute to the expression of kappa L chains in humans.
Feddersen
R M
RM
Department of Biochemistry, University of Iowa, Iowa City 52242.
Martin
D J
DJ
Van Ness
B G
BG
D000483:Alleles; D015139:Blotting, Southern; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics; D015328:Gene Rearrangement, B-Lymphocyte, Light Chain; D005803:Genes, Immunoglobulin; D006801:Humans; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D015448:Leukemia, B-Cell / Q000235:genetics; D010954:Plasmacytoma / Q000235:genetics; D011995:Recombination, Genetic; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D004273:DNA, Neoplasm; D007145:Immunoglobulin kappa-Chains