Distribution and co-existence of Met-enkephalin-like and mesotocin-like immunoreactivity in the neural lobe of the pituitary of the frog.
Content and distribution of Met-enkephalin (Met-ENK)-like immunoreactivity in the hypothalamo-hypophysial system of bovine, rat and frog were examined using specific radioimmunoassay and immunohistochemistry. Ultrastructural localization and co-existence of Met-ENK-, mesotocin (MT)- and vasotocin (VT)-like immunoreactivity in the neural lobe of the frog pituitary was examined by a method combining pre-embedding peroxidase-antiperoxidase immunostaining for Met-ENK with post-embedding immunocolloidal gold staining for MT or VT. The highest concentrations of immunoassayable Met-ENK were present in the neural lobe of the pituitary of the frog. In addition to nerve fibers showing only MT-like or Met-ENK-like immunoreactivity, nerve fibers containing neurosecretory granules showing both MT- and Met-ENK-like immunoreactivities were very rich. But VT-like and Met-ENK-like immunoreactivity was confirmed separately in different axon terminals.
Takeda
F
F
Department of Biochemistry, Showa University School of Medicine, Tokyo, Japan.
Takeda
M
M
Sato
T
T
Shioda
S
S
Nakai
Y
Y
D000818:Animals; D002417:Cattle; D004744:Enkephalin, Methionine / Q000378:metabolism*; D007031:Hypothalamus / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D010121:Oxytocin / Q000031:analogs & derivatives* / Q000378:metabolism; D010902:Pituitary Gland / Q000378:metabolism; D010904:Pituitary Gland, Posterior / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D014018:Tissue Distribution
C001713:mesotocin; D010121:Oxytocin; D004744:Enkephalin, Methionine