Expression of the Drosophila melanogaster metallothionein genes in yeast.
The metallothionein system in Drosophila melanogaster is composed of two genes, Mto and Mtn, that code for distinctly different proteins. In order to compare the properties of Mto and Mtn, we transformed yeast with several fusion plasmids. The Mto and Mtn cDNAs, when placed under the control of CUP1 or PGK promoters, can confer a copper-resistance phenotype to copper-hypersensitive cells. Both Mto and Mtn proteins can be characterized in extracts from transformed yeast cells.
Silar
P
P
Unité de Recherche Associée D1134 du Centre National de la Recherche Scientifique, Université Paris XI, Orsay, France.
Wegnez
M
M
D000818:Animals; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004331:Drosophila melanogaster / Q000235:genetics*; D015870:Gene Expression; D008668:Metallothionein / Q000235:genetics*; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics
D012333:RNA, Messenger; D011994:Recombinant Proteins; D008668:Metallothionein