[A new expressible VH-gene of the 36-40 family participates in the biosynthesis of antibodies against swine transferrin].
The expressible allelic variant of immunoglobulin heavy chain genes locus from hybridoma PTF-02 is localized in the 17 kb Bgl II fragment of DNA. The structure of VDJh 3 exon and flanking regions was investigated. The 1101 bp fragment was sequenced. It contains transcription regulatory sequences (cd and TTAAAT-box), the leader exon with internal intron (L), the Vh exon, D and Jh 3 segments. The Vh exon has unusual for immunoglobulin genes the first codon-threonine codon. It was shown that expressible in hybridoma PTF-02 Vh gene (Vh TF) represents and earlier unknown gene of the mouse 36-60 Vh family. This conclusion was made after the comparison of Vh TF exon structure and its 5'-region physical map with the other genes of the 36-60 family.
Urakov
D N
DN
Deev
S M
SM
Chernov
B K
BK
Polianovskiĭ
O L
OL
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation / Q000235:genetics*; D001483:Base Sequence; D005091:Exons; D005803:Genes, Immunoglobulin / Q000502:physiology*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010957:Plasmids; D015183:Restriction Mapping; D013552:Swine; D014168:Transferrin / Q000276:immunology*
D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D014168:Transferrin
Novyĭ ékspressiruemyĭ VH-gen semeĭstva 36-60 uchastvuet v biosinteze antitel protiv svinogo transferrina.