Development of a high-performance electrophoretic light scattering apparatus for mobility determination of particles with their Stokes' radii of several nanometers.
A sophisticated electrophoretic light scattering apparatus was developed. It allows reliable measurements of electrophoretic mobilities of charged macromolecules, such as proteins, nucleic acids and synthetic polyelectrolytes which have been out of the range of the apparatuses of this type aimed at relatively large particles such as microspheres and biological cells. The present apparatus features the monitoring of the electroosmotic flow profile and the determination of the sign of the electrophoretic mobility of an object as well as its high-performance based on the improvement in various facets of acquisition and handling of the signals and data.
Oka
K
K
Otsuka Electronics Co. Ltd., Shiga, Japan.
Otani
W
W
Kameyama
K
K
Kidai
M
M
Takagi
T
T
D004058:Diffusion; D004553:Electric Conductivity; D001330:Electronic Data Processing; D004586:Electrophoresis; D010316:Particle Size; D013053:Spectrophotometry