title: Partial amino acid sequence of erythrocyte carbonic anhydrase from tiger shark.
abstract: 1. A partial primary structure (197 residues) of carbonic anhydrase from tiger shark (Galeocerdo cuvieri) erythrocytes has been determined. 2. The amino acid sequence of the enzyme is identical to those of human cytoplasmic carbonic anhydrases (CA I-III) by as much as 52-60%. 3. It is shown that tiger shark CA most closely resembles the CA II isoenzyme of amniotes. 4. Isoelectric focusing and inhibition studies on carbonic anhydrase from dogfish (Squalus acanthias) blood and muscle indicate the presence of the same isoenzyme in shark blood and muscle.
authors.affiliation: IFM/Department of Chemistry, Linköping University, Sweden.
authors.forename: N
authors.initials: N
authors.lastname: Bergenhem
authors.forename: U
authors.initials: U
authors.lastname: Carlsson
mesh_terms: D000478:Alkylation; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002257:Carbonic Anhydrase Inhibitors / Q000494:pharmacology; D002256:Carbonic Anhydrases / Q000097:blood* / Q000302:isolation & purification; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004284:Dogfish / Q000097:blood; D010450:Endopeptidases; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007525:Isoelectric Focusing; D008272:Mafenide / Q000494:pharmacology; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D012588:Scintillation Counting; D012697:Serine Endopeptidases; D012754:Sharks / Q000097:blood*; D013045:Species Specificity; D014357:Trypsin
chemical_list: D000596:Amino Acids; D002257:Carbonic Anhydrase Inhibitors; D008272:Mafenide; D010450:Endopeptidases; C044499:submandibular proteinase A; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D014357:Trypsin; D002256:Carbonic Anhydrases