title: [Properties of two Bacillus subtilis bacteriophages].
abstract: Two lytic bacteriophages of Bacillus subtilis BF7658 were isolated. Their morphology, biological properties and physiochemical characteristics of their DNAs were compared. Electromicroscopic observation indicated that BS31 has a hexagonal head and contracted tail sheath, BS32 is a complex short-tailed phage and similar to phi 29 but differs from phi 29 in size and other properties. Two phages have a narrow spectrum of host range. The molecular weight of BS31 and BS32 DNAs are 62kb and 17kb respectively by EcoR1 endonuclease analysis. The G + C content of the DNAs are 45.7%(BS31) and 40.7%(BS32). Structural proteins of BS31 gave two major bands and at least ten minor ones; BS32 gave three major bands and six minor ones by SDS-polyacralamide gel electrophoresis.
authors.affiliation: Institute of Microbiology, Academia Sinica, Beijing.
authors.forename: S M
authors.initials: SM
authors.lastname: Na
authors.forename: P X
authors.initials: PX
authors.lastname: Jia
authors.forename: M X
authors.initials: MX
authors.lastname: Yu
mesh_terms: D001412:Bacillus subtilis; D001435:Bacteriophages / Q000302:isolation & purification* / Q000648:ultrastructure; D004269:DNA, Bacterial / Q000032:analysis
chemical_list: D004269:DNA, Bacterial