Characterization of a new T-lineage glycoprotein expressed in mature T-cell leukemias and lymphomas.
We identified a new human, T-lineage restricted glycoprotein of molecular weight 120 Kd that is expressed primarily in mature T-cell malignancies. The antigen, named TCA-1 (T-cell cytoplasmic antigen), is an intracellular glycoprotein found mainly in the Golgi stacks, although a few cell lines also display surface membrane TCA-1. Many but not all T-cell neoplasms express this antigen. The antigen is absent from neoplastic and normal human tissue outside the T-lymphocyte lineage. TCA-1 was identified by murine monoclonal antibodies produced after immunization of mice with T-cell chronic lymphocytic leukemia cells. The glycoprotein is a monomer containing approximately 4% N-linked carbohydrate with terminal D-galactose residues. Partial amino acid sequence analysis of TCA-1 shows homology with an immunoglobulin heavy chain region, which suggests that TCA-1 may belong to the immunoglobulin supergene family of receptor and adhesion molecules.
Cassano
W F
WF
Division of Pediatric Hematology-Oncology, University of Florida, Gainesville 32610.
D000595:Amino Acid Sequence; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis*; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D015151:Immunoblotting; D007143:Immunoglobulin Heavy Chains; D007938:Leukemia; D008223:Lymphoma; D008562:Membrane Glycoproteins / Q000032:analysis; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000032:analysis; D012016:Reference Values; D012689:Sequence Homology, Nucleic Acid; D013601:T-Lymphocytes / Q000032:analysis*; D014407:Tumor Cells, Cultured / Q000032:analysis*
D000945:Antigens, Differentiation, T-Lymphocyte; D000951:Antigens, Neoplasm; D006023:Glycoproteins; D007143:Immunoglobulin Heavy Chains; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins