[A simplified polygraphic method for studying sleep in the rat].
Experiments were performed on 10 healthy Wistar rats with chronically implanted electrodes for the recording of electrocorticogram (ECoG), electro-oculogram (EOG), and electromyography (EMG). All the rats were prepared for the implantation of recording electrodes under 5% Ketamine (100 mg/kg i.p.) anesthesia. Four to six days after operation, the rat was put in an apparatus which was designed specifically sensitive to detect the movement of the animal without electrode on the body so that electroactogram (EAG) could be recorded readily. One group of experiment animal (n = 5), recorded ECoG, EAG, EOG and EMG simultaneously, was compared to another group (n = 5), recorded ECoG and EAG simultaneously. The results of experiments indicate that the ECoG, recorded from the the mesial frontal and occipital areas, have certain characteristics during different sleep phases, and the characteristics for classifying sleep phases by the two kinds of somnopolygram are similar. Therefore, simultaneous recording of the ECoG and EAG can be used to discriminate the sleep phases in the rat. In this way, the somnopolygraphic recording method for studying sleep in the rat would be simplified.
Yang
J
J
Xu
J
J
Yang
S
S
D000818:Animals; D004569:Electroencephalography; D004576:Electromyography; D004585:Electrooculography; D005260:Female; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012890:Sleep / Q000502:physiology*; D012895:Sleep, REM / Q000502:physiology