title: Purification and characterization of a 43-kDa protease of Bacteroides gingivalis.
abstract: From the culture supernatant of Bacteroides gingivalis ATCC 33277, a thiol protease was purified to homogeneity by fractional ammonium sulfate precipitation, ion-exchange chromatography, gel filtration, and isoelectric focusing. Its molecular weight was 43 kDa and showed similar enzymatic properties to a 300-kDa protease that was previously characterized.
authors.affiliation: Matsumoto Dental College, Hiro'oka, Shiojiri, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Fujimura
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
mesh_terms: D001439:Bacteroides / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000302:isolation & purification*; D008970:Molecular Weight
chemical_list: D010450:Endopeptidases