Specific mRNAs accumulate in long-wavelength UV-irradiated mouse epidermis.
Total RNA from hairless mice epidermis was analysed by RNA-DNA hybridization on slot blots at various times following irradiation with long-wavelength UV light (UV-B or UV-B plus UV-A). Densitometric scanning of the autoradiographies indicates that the amount of RNA which hybridizes with an Ha-ras DNA probe remains constant for up to 72 h after irradiation. The amount of the transcript which hybridizes to a c-fos DNA probe increases dose dependently from 15 min to 2 h after irradiation. RNA which hybridizes to a tubulin DNA probe accumulates in parallel with the increase in mitotic index associated with the radiation-induced hyperplasia.
Brunet
S
S
Department of Biology, Laboratoire de Recherche Fondamentale de l'Oréal, Aulnay-sous-Bois, France.
Giacomoni
P U
PU
D000818:Animals; D002478:Cells, Cultured; D015342:DNA Probes; D004817:Epidermis / Q000378:metabolism / Q000528:radiation effects*; D011905:Genes, ras / Q000187:drug effects; D015603:Keratinocytes / Q000528:radiation effects; D051379:Mice; D008812:Mice, Hairless; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D014158:Transcription, Genetic / Q000528:radiation effects; D014404:Tubulin / Q000235:genetics; D014466:Ultraviolet Rays
D015342:DNA Probes; D012333:RNA, Messenger; D014404:Tubulin