Superposition of arteriolar vasomotion waves and regulation of blood flow in skeletal muscle microcirculation.
In skin muscle microcirculation of Syrian hamsters, rhythmic diameter changes were studied along the arteriolar network, under normoxic conditions, at rest. A teflon coated-aluminum chamber was implanted in the dorsum skin of animals. The microcirculation was investigated using intravital microscopy technique. Vessel diameters were determined by a computer-assisted method. Power spectrum analysis of vasomotion recordings was carried out with Fast Fourier Transform and Autoregressive modelling. To determine vasomotion waveform spreading, cross-spectral data (amplitude and phase) were computed, using the modified periodogram method (FFT). The arterioles were classified according to Strahler's method. Order 1 vessels (diameter: 7.50 +/- 1.16 microns) showed the highest frequency, 4-15 cycles per min, and percentage amplitude in the range 60-100%. Order 2 and 3 arterioles had intermediate frequencies, and amplitude in the range 50-100%, and 15-50%, respectively. The largest order 4 vessels (diameter: 28.97 +/- 9.55 microns) had the lowest frequency, 0.3-3 cpm, and amplitude in the range 5-20%. In most networks, cross-correlation analysis revealed two groups of frequency components. Low frequency group was propagated from order 4 and 3 vessels downstream. High frequency components were transmitted upstream from order 1 and 2 arterioles. Therefore, a complex superposition of waveforms resulted from the activity of discrete points along the microvasculature. In conclusion, rhythmic diameter changes of arterioles in skeletal muscle microcirculation regulate blood flow distribution in capillary units and control tissue oxygenation.
Colantuoni
A
A
CNR Institute of Clinical Physiology, University of Pisa, Italy.
Bertuglia
S
S
Coppini
G
G
Donato
L
L
D000818:Animals; D001160:Arterioles / Q000033:anatomy & histology / Q000502:physiology; D006224:Cricetinae; D008297:Male; D008647:Mesocricetus; D008833:Microcirculation / Q000033:anatomy & histology / Q000502:physiology; D009068:Movement / Q000502:physiology; D009132:Muscles / Q000098:blood supply*; D012039:Regional Blood Flow / Q000502:physiology; D014661:Vasoconstriction / Q000502:physiology; D014664:Vasodilation / Q000502:physiology