Coordinated induction of two unrelated glucose-regulated protein genes by a calcium ionophore: human BiP/GRP78 and GAPDH.
The induction of human BiP/GRP78 and GAPDH protein genes by the calcium ionophore A23187 was determined. Steady-state levels of mRNA for both the glucose starvation-responsive BiP/GRP78 gene and the glucose-responsive GAPDH gene were dramatically induced in a variety of human cells. There is a homologous palindromatic sequence GCCGTTAACGGC in the active promoter region of the two genes that is known to be required for the induction of mammalian BiP/GRP78 by A23187. The evidence confirms in general the function of this element in the regulation of calcium-associated gene activity.
Chao
C C
CC
Department of Biochemistry, Chang Gung Medical College, Taiwan, Republic of China.
Yam
W C
WC
Lin-Chao
S
S
D001483:Base Sequence; D015152:Blotting, Northern; D000001:Calcimycin / Q000494:pharmacology*; D002352:Carrier Proteins / Q000235:genetics*; D002460:Cell Line; D000091342:Endoplasmic Reticulum Chaperone BiP; D005786:Gene Expression Regulation / Q000187:drug effects*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics*; D006360:Heat-Shock Proteins; D006801:Humans; D066298:In Vitro Techniques; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid
D002352:Carrier Proteins; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D012333:RNA, Messenger; D000001:Calcimycin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases