[Evaluation of the relative effectiveness of hybridization probes prepared by action of hydrazides of biotin derivatives on DNA].
Efficiencies of biotinylated DNAs as hybridisation probes in a model system of non-radioactive detection were compared. Probes were obtained by interaction of single-stranded DNA and each of four different hydrazides of biotin derivatives. The most sensitivity in detection of complementary target was obtained using (biocytin hydrazide)-treated DNA. Relations between hydrazide structures and sensitivity of biotinylated probes are discussed.
Karpyshev
N N
NN
Bondarenko
T Iu
TIu
Kipriianov
S M
SM
D001710:Biotin; D055598:Chemical Phenomena; D002621:Chemistry; D015342:DNA Probes / Q000138:chemical synthesis*; D006834:Hydrazines; D009693:Nucleic Acid Hybridization
D015342:DNA Probes; D006834:Hydrazines; D001710:Biotin
Otsenka otnositel'noĭ éffektivnosti gibridizatsionnykh zondov, poluchennykh deĭstviem gidrazidov proizvodnykh biotina na DNK.