Immunochemical analysis of adrenocortical cytochrome P450scc: 1. Monoclonal antibodies against haemprotein.
Twelve stable mouse hybridoma cell lines producing monoclonal antibodies against bovine adrenocortical cytochrome P450scc were prepared. All the monoclonal antibodies interacted specifically with the antigen as shown by radioimmunoassay (RIA), enzyme-linked immunosorbent assay (ELISA), dot-immunoblotting, and Western blot analysis, but did not precipitate in Ouchterlony double-immunodiffusion analysis. A highly sensitive competitive RIA based on monoclonal and polyclonal mouse antibodies was developed to determine the total content of P450scc in adrenocortical mitochondria. Three of the monoclonal antibodies strongly inhibited the conversion of cholesterol to pregnenolone.
Efimov
A V
AV
Institute of Bioorganic Chemistry, Academy of Sciences of the Belorussian SSR, Minsk.
Chernogolov
A A
AA
Petrashin
A I
AI
Kiril'chik
E P
EP
Usanov
S A
SA
Chashchin
V L
VL
D000302:Adrenal Cortex / Q000201:enzymology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D015153:Blotting, Western; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006420:Hemeproteins / Q000276:immunology*; D006825:Hybridomas; D015151:Immunoblotting; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D006420:Hemeproteins; D002786:Cholesterol Side-Chain Cleavage Enzyme