Spatio-temporal distribution of the adherens junction-associated molecules vinculin and talin in the early avian embryo.
To gain an insight into the possible involvement of the cytoskeletal components and cellular junctions in morphogenetic processes during development, we have studied the spatio-temporal distribution of two major adherens-junction-associated molecules, vinculin and talin, during avian embryogenesis, using immunofluorescence microscopy and immunoblotting. Both molecules were detected at very early stages during morphogenesis and were found in a wide variety of tissues deriving from the three primary germ layers. A number of tissues, including smooth and striated muscles, endothelia, and some hemopoietic precursors, expressed vinculin and talin at especially high levels either transiently or permanently. Conversely, only a few cell types, e.g., circulating erythrocytes and neurones in the central nervous system lacked or expressed them at very low levels. In addition, expression of vinculin and talin was in some cases modulated in connection with morphological rearrangements of tissues. In particular, they were transiently enhanced in restricted areas of the ectoderm and endoderm undergoing extensive foldings. However, other morphogenetic events such as local disruptions of epithelia were not accompanied by extensive modifications in their expression. Finally, it appeared that, in most cases, vinculin and talin overlapped in their distribution, and the level of their expression was regulated coincidently with the notable exceptions of the primordium of the central nervous system, the nephron, and the liver where each molecule followed independent regulatory patterns. It appears from this study that the spatio-temporal distribution of vinculin and talin correlates frequently with that of the adhesion molecules A-CAM (or N-cadherin), L-CAM, and of integrin receptors. Thus, vinculin and talin, in association with the membrane components of adherens junctions, may actively participate both in the control of cellular interactions during early embryonic development and in cell differentiation.
Duband
J L
JL
Laboratoire de Physiopathologie du Développement, Centre National de la Recherche Scientifique et Ecole Normale Supérieure, Paris, France.
Thiery
J P
JP
D000818:Animals; D002319:Cardiovascular System / Q000196:embryology; D003370:Coturnix / Q000196:embryology* / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000096:biosynthesis*; D005775:Gastrula / Q000378:metabolism; D006321:Heart / Q000196:embryology; D006413:Hematopoietic System / Q000378:metabolism; D007150:Immunohistochemistry; D007365:Intercellular Junctions / Q000378:metabolism*; D009024:Morphogenesis / Q000502:physiology; D009132:Muscles / Q000196:embryology; D009420:Nervous System / Q000196:embryology; D011784:Quail / Q000196:embryology*; D016608:Talin; D016596:Vinculin
D003598:Cytoskeletal Proteins; D016608:Talin; D016596:Vinculin