Occurrence of two germline-related rheumatoid factor idiotypes in rheumatoid arthritis and in non-rheumatoid seropositive individuals.
Human rheumatoid factor (RF) paraproteins express two distinct light chain cross-reactive idiotypes defined by the monoclonal antibodies 17.109 and 6B6.6. These germline gene-related cross-reactive idiotypes are both carried on VK3 light chains and are each present on about one-third of IgM RF paraproteins. We assessed the degree to which these idiotypes are represented in polyclonal RFs. We used rheumatoid arthritis (RA) and non-RA RF-positive sera selected from a large cross-sectional population study (the Mini-Finland Health Survey), and sera from a community-based follow-up study of recent-onset RA patients from Heinola, Finland. In the Mini-Finland Health Survey, elevated levels of the 17.109 RF idiotype were seen in sera of 13% of the RA and 19% of the non-RA group; 6B6.6 RF was seen in 26% of the RA and 28% of the non-RA group. In sera of the Heinola follow-up study, 17.109 RF was seen in 12% initially, but in only 3% at 8 years. Similarly, 6B6.6 RF was detected in 25% initially, but in only 7% at 8 years. Ten sera positive for RF prior to the onset of clinical RA were identified from individuals of a second large population study from Finland (North Karelia project); two of these sera exhibited the 6B6.6 idiotype; none exhibited the 17.109 idiotype. The data are consistent with the concept that these germline gene-related cross-reactive RF idiotypes occur frequently in the polyclonal RF of non-RA as well as RA sera, and that in RA the idiotypes may sometimes be reduced or lost as a consequence of somatic diversification of the RF through somatic mutation, usage of new germline genes, or both.
Kouri
T
T
Department of Molecular and Experimental Medicine, Research Institute of Scripps Clinic, La Jolla, CA.
Crowley
J
J
Aho
K
K
Palosuo
T
T
Isomäki
H
H
von Essen
R
R
Heliövaara
M
M
Carson
D
D
Vaughan
J H
JH
D001172:Arthritis, Rheumatoid / Q000235:genetics / Q000276:immunology*; D015331:Cohort Studies; D003429:Cross Reactions; D003430:Cross-Sectional Studies; D005387:Finland; D005500:Follow-Up Studies; D005803:Genes, Immunoglobulin; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000032:analysis / Q000235:genetics / Q000276:immunology; D012217:Rheumatoid Factor / Q000032:analysis* / Q000235:genetics / Q000276:immunology
D007130:Immunoglobulin Idiotypes; D007147:Immunoglobulin Light Chains; D012217:Rheumatoid Factor