Differential susceptibility of metastatic lymphoma cells to natural immunity.
The susceptibility of metastatic variant lymphoma cells to natural immunity was studied using a low malignant/metastatic parental RAW117-P cell line and its liver colonizing highly malignant/metastatic RAW117-H10 cell line. The metastatic variant RAW117-H10 cells express a significantly lower amount of laminin-like and fibronectin-like molecules as determined by flow cytometry using monospecific polyclonal antibodies to laminin and fibronectin. Our studies indicated that the RAW117-H10 cells are resistant to natural killer (NK) cell-mediated cytotoxicity. In vitro activation of the effector cells with interferon-gamma increased the susceptibility of these cells to NK-mediated cytotoxicity while maintaining the difference between the two cell lines. However, when recombinant interleukin-2 was used to activate the effector cells, the cytotoxicity of the lymphokine-activated effector cells to both parental low metastatic RAW117-P cells and highly metastatic RAW117-H10 cells was similar.
Hao
W M
WM
Department of Anatomy, University of Nebraska Medical Center, Omaha.
Joshi
S S
SS
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D003602:Cytotoxicity, Immunologic; D005353:Fibronectins / Q000032:analysis; D005434:Flow Cytometry; D007113:Immunity, Innate; D007371:Interferon-gamma / Q000502:physiology; D007376:Interleukin-2 / Q000502:physiology; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology; D007797:Laminin / Q000032:analysis; D008223:Lymphoma / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009362:Neoplasm Metastasis / Q000276:immunology; D014407:Tumor Cells, Cultured / Q000276:immunology
D000954:Antigens, Surface; D005353:Fibronectins; D007376:Interleukin-2; D007797:Laminin; D007371:Interferon-gamma