Characterization and partial purification of human epithelial transforming growth factor.
A polypeptide growth factor has been partially purified from medium conditioned by the human adrenocortical carcinoma cell line SW13. This factor, designated h-TGFe, stimulates anchorage-independent growth of the SW13 cells. Similar activity was observed in human milk, and in conditioned media from seven of 14 epithelial cell lines. The SW13-derived activity is stable to low pH and 8M urea but labile to dithiothreitol and 2% sodium dodecyl sulfate. Human TGFe does not bind to heparin and fails to stimulate growth of endothelial cells in monolayer culture. The apparent molecular weight of h-TGFe is 59k by size exclusion chromatography in the presence of 8M urea and the activity binds strongly to cation exchangers. The activity elutes at 15-30% acetonitrile from a C18 reverse-phase column and has been partially purified by using a four-step chromatographic procedure. TGFe appears to be a novel growth factor produced by many epithelial cells and tissues.
Dunnington
D J
DJ
Department of Cell Sciences, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania 19406-2799.
Scott
R G
RG
Anzano
M A
MA
Greig
R
R
D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004229:Dithiothreitol / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000032:analysis / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008895:Milk, Human / Q000737:chemistry; D008970:Molecular Weight; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D015291:Transforming Growth Factors / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D012967:Sodium Dodecyl Sulfate; D005346:Fibroblast Growth Factors; D015291:Transforming Growth Factors; D004229:Dithiothreitol