title: A simple serum galactosyltransferase assay method suitable for routine use.
abstract: UDP-Galactose: N-acetylglucosaminyl glycoprotein beta 1-4 galactosyltransferase (GT) catalyzes the transfer of galactose to N-acetylglucosamine from UDP-[3H]Gal. The uncharged reaction product (tritiated N-acetyllactosamine) is separated from the unreacted UDP-[3H]Gal by ion-exchange chromatography. The major advantage of this method is its rapidity compared to other isotopic techniques.
authors.affiliation: Laboratoire de Biochimie Clinique et Expérimentale, Centre F. Baclesse Caen, France.
authors.forename: F
authors.initials: F
authors.lastname: Sichel
authors.forename: J P
authors.initials: JP
authors.lastname: Malas
authors.forename: P
authors.initials: P
authors.lastname: Gauduchon
authors.forename: M
authors.initials: M
authors.lastname: Aubert
authors.forename: E
authors.initials: E
authors.lastname: Bar
authors.forename: J Y
authors.initials: JY
authors.lastname: Le Talaër
mesh_terms: D002852:Chromatography, Ion Exchange; D005260:Female; D006801:Humans; D007788:Lactose Synthase / Q000097:blood*; D008297:Male; D009237:N-Acetyllactosamine Synthase / Q000097:blood*; D012016:Reference Values
chemical_list: D007788:Lactose Synthase; D009237:N-Acetyllactosamine Synthase