Correlative light microscopy, scanning electron microscopy, and transmission electron microscopy of osmium-macerated biological tissues.
A method facilitating correlation of light microscopic (LM), scanning electron microscopic (SEM) and transmission electron microscopic (TEM) images was developed. Rat kidney and heart were initially subjected to the osmium maceration procedure and then embedded in acrylic resin. Semithin sections of the tissue blocks were first provided for LM and then examined by SEM after resin removal. Furthermore, the ultrathin sections adjacent to the semithin sections were observed by TEM. The three-dimensional images of intracellular organelles provided an informative adjunct to LM and TEM.
Scala
C
C
Institute of Clinical Electron Microscopy, University of Bologna, S. Orsola Hospital, Italy.
Cenacchi
G
G
Apkarian
R P
RP
Preda
P
P
Pasquinelli
G
G
D000818:Animals; D007668:Kidney / Q000648:ultrastructure*; D008854:Microscopy, Electron / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D009206:Myocardium / Q000648:ultrastructure*; D009992:Osmium; D051381:Rats
D009992:Osmium