Characterization of DOPA decarboxylase mRNA in rat pheochromocytoma.
Total poly (A+) RNA has been extracted from rat pheochromocytoma and translated in vitro by means of a reticulocyte lysate system. We show that two antisera, prepared against pig kidney DOPA decarboxylase (DDC) or rat pheochromocytoma DDC, immunoprecipitate an in vitro synthetized 50 kDa polypeptide identified as DDC by competition experiments with pure DDC. The proportion of specific mRNA has been calculated and represents 0.05% of total poly A+ mRNA. Its size has been established by electrophoresis in methylmercuric hydroxide containing agarose gel, corresponding to a 2.2 kb length mRNA.
Bruneau
G
G
Laboratoire de Biochimie Cellulaire, Collège de France, Paris.
Krieger-Poullet
M
M
Coge
F
F
Borri-Voltattorni
C
C
Gros
F
F
Thibault
J
J
D000818:Animals; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000235:genetics*; D004296:Dopa Decarboxylase / Q000096:biosynthesis / Q000235:genetics*; D066298:In Vitro Techniques; D007668:Kidney / Q000201:enzymology; D010673:Pheochromocytoma / Q000201:enzymology*; D011061:Poly A / Q000032:analysis; D011233:Precipitin Tests; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D013552:Swine
D012333:RNA, Messenger; D011061:Poly A; D004296:Dopa Decarboxylase; D001142:Aromatic-L-Amino-Acid Decarboxylases