Structure and expression of a light-inducible shoot-specific rice gene.
By differential screening of a cDNA library of two-week-old rice seedlings cDNA clones were obtained, corresponding to shoot-specific mRNAs. By sequence analysis two of these clones were found to be rbcS cDNA clones. The mRNA corresponding to a third cDNA clone (COS5) displayed an expression pattern similar to the expression pattern of rbcS genes. The mRNA (800 bases) was light-inducible and encoded by a single-copy gene. The genomic clone (GOS5) was isolated and the intron/exon structure was determined by comparing the nucleotide sequences of the mRNA and the genomic clone. The gene contains two introns. Transcription start sites were determined by S1-nuclease mapping and primer extension. The start site obtained by both methods is located 87 bp upstream of the translation start site and 23 bp downstream of TATA box-like sequence. In the 5' non-coding region motifs can be found that are homologous to sequences in promoters that are light- or UV-inducible or confer leaf-specific expression. The open reading frame present in GOS5 codes for a protein (15 kDa) that contains a putative chloroplast transit peptide and does not show any significant homology to protein sequences in the NBRF protein database.
de Pater
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MOLBAS Research Group, Department of Plant Molecular Biology, Leiden University, Netherlands.
Hensgens
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Schilperoort
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RA
D000595:Amino Acid Sequence; D001483:Base Sequence; D002736:Chloroplasts / Q000378:metabolism; D016384:Consensus Sequence; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation / Q000528:radiation effects; D017343:Genes, Plant; D008027:Light; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012275:Oryza / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D011499:Protein Processing, Post-Translational; D012273:Ribulose-Bisphosphate Carboxylase / Q000096:biosynthesis / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D010940:Plant Proteins; D004247:DNA; D012273:Ribulose-Bisphosphate Carboxylase