title: Comparative evaluation of three tests for the detection of Escherichia coli cytotoxic necrotizing factors (CNF1 and CNF2) using filtrates of cultures treated with mitomycin C.
abstract: Necrotizing Escherichia coli (NTEC) strains grown in the presence of mitomycin C released cell associated necrotizing factors CNF1 and CNF2 to culture medium. Using culture filtrates from 96 mitomycin C treated E. coli strains, we have found that a modified HeLa cell assay was a more sensitive and specific method for the detection of CNF1 and CNF2 than the Vero cell assay and the rabbit skin test.
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Veterinaria, Universidad de Santiago, Lugo, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Blanco
authors.forename: M
authors.initials: M
authors.lastname: Blanco
authors.forename: E A
authors.initials: EA
authors.lastname: González
authors.forename: M P
authors.initials: MP
authors.lastname: Alonso
authors.forename: J I
authors.initials: JI
authors.lastname: Garabal
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis*; D003470:Culture Media; D003603:Cytotoxins / Q000032:analysis*; D004926:Escherichia coli / Q000032:analysis* / Q000187:drug effects; D029968:Escherichia coli Proteins; D006367:HeLa Cells; D006801:Humans; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology*; D011817:Rabbits; D012882:Skin Tests; D014709:Vero Cells
chemical_list: D001427:Bacterial Toxins; D003470:Culture Media; D003603:Cytotoxins; D029968:Escherichia coli Proteins; D008937:Mitomycins; C060296:cytotoxic necrotizing factor type 1; D016685:Mitomycin