title: Tumor necrosis factor alpha induction in human monocytes.
abstract: The present study was undertaken to assess the presence of tumor necrosis factor (TNF)-alpha mRNA and protein in circulating human blood monocytes and to study the TNF-alpha gene expression in human monocytes isolated by continuous Percoll gradient fractionation. The technique of RNA isolation directly from the blood samples was used to study TNF-alpha mRNA expression in circulating human blood leukocytes. It was shown that human blood leukocytes of healthy donors contained no presynthesized pool of TNF-alpha mRNA as well as no TNF-alpha protein. It was found that early pretreatment with cycloheximide interferes with TNF-alpha mRNA induction by Staphylococcus aureus.
authors.affiliation: Byelorussian Institute of Hematology and Blood Transfusion, Minsk, USSR.
authors.forename: N I
authors.initials: NI
authors.lastname: Misuno
authors.forename: O A
authors.initials: OA
authors.lastname: Osipovich
authors.forename: A B
authors.initials: AB
authors.lastname: Sudarikov
authors.forename: G L
authors.initials: GL
authors.lastname: Idelson
authors.forename: T S
authors.initials: TS
authors.lastname: Kolesnikova
authors.forename: A V
authors.initials: AV
authors.lastname: Panyutich
authors.forename: N N
authors.initials: NN
authors.lastname: Voitenok
mesh_terms: D001681:Biological Assay; D015152:Blotting, Northern; D002469:Cell Separation; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D012016:Reference Values; D014158:Transcription, Genetic / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D003513:Cycloheximide