Growth inhibition of human fibroblasts by epidermal growth factor in the presence of arachidonic acid.
The effect of epidermal growth factor on growth of human fibroblasts was investigated in serum-free medium supplemented with various fatty acids. When linoleic acid, arachidonic acid, or eicosapentaenoic acid was added, each inhibited epidermal growth factor-induced cell growth and showed cytotoxicity at high concentrations (greater than 10 microM). This cytotoxic effect was not observed in the presence of indomethacin, suggesting that prostaglandin production is important in mediation of the growth inhibition. Prostaglandin E2 was increased more than ten thousand times by epidermal growth factor in combination with arachidonic acid.
Hori
T
T
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
Yamanaka
Y
Y
Hayakawa
M
M
Shibamoto
S
S
Oku
N
N
Ito
F
F
D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D015232:Dinoprostone / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D015118:Eicosapentaenoic Acid / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology
D001095:Arachidonic Acids; D008041:Linoleic Acids; D016718:Arachidonic Acid; D004815:Epidermal Growth Factor; D019787:Linoleic Acid; D015118:Eicosapentaenoic Acid; D015232:Dinoprostone; D007213:Indomethacin