Separation and some properties of an endo-1,4-beta-D-xylanase from Aspergillus flavipes.
A fungal strain isolated from stored wheat straw and identified as Aspergillus flavipes was found to produce an extracellular endo-1,4,beta-D-xylanase when grown in shake-culture of a mineral salt medium containing 0.1% wheat strawxylan as a sole source of carbon for 3 days at 28 degrees C. The enzyme was separated by precipitation with ammonium sulphate and desalted by Sephadex G-25 column. Fractionation and purification of the enzyme were carried out by chromatography on Bio-Gel P-100. The purified enzyme (mol wt 45,000) was found to release xylooligomers only from beta-1,4-xylan. It showed maximum activity at pH 5.0 and temperature 55 degrees C.
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Botany Department, Faculty of Science, Mansoura University, Egypt.
D001230:Aspergillus / Q000201:enzymology* / Q000254:growth & development; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D043364:Endo-1,4-beta Xylanases; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification*; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D013379:Substrate Specificity; D013696:Temperature; D014908:Triticum / Q000382:microbiology
D005656:Fungal Proteins; D006026:Glycoside Hydrolases; D043364:Endo-1,4-beta Xylanases