Roles of CD4+ and CD8+ cells, and the effect of administration of recombinant murine interferon gamma in listerial infection.
Studies were made on the effects of in vivo administration of anti-CD4 mAb, anti-CD8 mAb, or a combination of both mAbs on multiplication of bacteria, the levels of serum transaminases, and mortality in mice infected with Listeria monocytogenes. Results showed that in sublethal infection, CD8+ cells enhanced the peak of bacterial multiplication and liver cell necrosis, and CD4+ cells suppressed CD8+ cell-mediated enhancement. Results also showed that either CD4+ or CD8+ cells were necessary for, and capable of, mediating clearance of the bacteria. CD8+ cells were more efficient than CD4+ cells, but for optimal clearance both were necessary. In lethal listeriosis, treatment of mice with anti-CD8 mAb or a combination of both anti-CD4 and anti-CD8 mAbs, but not anti-CD4 mAb only, protected mice from death by decreasing multiplication of bacteria in the liver and spleen after a peak of approximately 10(8) CFU, and lowering the elevated serum levels of transaminases. These findings indicated that CD8+ cells were responsible for causing irreversible systemic Listeria infection and severe liver necrosis. In lethal listeriosis, administration of rMuIFN-gamma markedly prolonged survival by decreasing multiplication of bacteria and promoting recovery from liver necrosis.
Sasaki
T
T
Department of Laboratory Medicine, Nagasaki University School of Medicine, Japan.
Mieno
M
M
Udono
H
H
Yamaguchi
K
K
Usui
T
T
Hara
K
K
Shiku
H
H
Nakayama
E
E
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D001219:Aspartate Aminotransferases / Q000097:blood; D015704:CD4 Antigens / Q000276:immunology*; D016827:CD8 Antigens; D003165:Complement System Proteins / Q000276:immunology; D005260:Female; D007111:Immunity, Cellular; D007371:Interferon-gamma / Q000627:therapeutic use*; D008088:Listeriosis / Q000276:immunology* / Q000473:pathology / Q000628:therapy; D008099:Liver / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009336:Necrosis; D011994:Recombinant Proteins; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D015704:CD4 Antigens; D016827:CD8 Antigens; D011994:Recombinant Proteins; D007371:Interferon-gamma; D003165:Complement System Proteins; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase