title: Sequences of the genes encoding argininosuccinate synthetase in Escherichia coli and Saccharomyces cerevisiae: comparison with methanogenic archaebacteria and mammals.
abstract: The nucleotide (nt) sequences of the genes encoding argininosuccinate synthetase from Escherichia coli K-12 (argG) and Saccharomyces cerevisiae (ARG1) were determined. The deduced amino-acid sequences were compared to each other and to their counterparts in two methanogens and in mammals. Three regions are highly conserved. Two of them appear to contain possible Walker-type nt-binding sites [Walker et al., EMBO J. 1 (1982) 945-951] and are therefore candidates for ATP-binding sites. The third region shows some similarity to a short portion of the N-proximal part of the PurA enzyme which catalyses an analogous reaction.
authors.affiliation: Vrije Universiteit Brussel, Belgium.
authors.forename: F
authors.initials: F
authors.lastname: Van Vliet
authors.forename: M
authors.initials: M
authors.lastname: Crabeel
authors.forename: A
authors.initials: A
authors.lastname: Boyen
authors.forename: C
authors.initials: C
authors.lastname: Tricot
authors.forename: V
authors.initials: V
authors.lastname: Stalon
authors.forename: P
authors.initials: P
authors.lastname: Falmagne
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: S
authors.initials: S
authors.lastname: Baumberg
authors.forename: N
authors.initials: N
authors.lastname: Glansdorff
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001105:Archaea / Q000235:genetics; D001124:Argininosuccinate Synthase / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005800:Genes, Fungal; D008322:Mammals / Q000235:genetics; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D001124:Argininosuccinate Synthase