title: Recognition of tRNA identity determinants by aminoacyl-tRNA synthetases.
abstract: By analyzing aminoacylation activities of variants of isoleucine tRNAs and glutamic acid tRNA from Escherichia coli, it was found that the anticodons are the major determinants for "identities" of these tRNAs. It was also shown that the post-transcriptional modifications are essential to aminoacylation of these tRNAs. Interactions between the tRNAs and the aminoacyl-tRNA synthetases were analyzed by NMR spectroscopy.
authors.affiliation: Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Muramatsu
authors.forename: O
authors.initials: O
authors.lastname: Nureki
authors.forename: H
authors.initials: H
authors.lastname: Kanno
authors.forename: T
authors.initials: T
authors.lastname: Niimi
authors.forename: M
authors.initials: M
authors.lastname: Tateno
authors.forename: T
authors.initials: T
authors.lastname: Kohno
authors.forename: G
authors.initials: G
authors.lastname: Kawai
authors.forename: T
authors.initials: T
authors.lastname: Miyazawa
authors.forename: Y
authors.initials: Y
authors.lastname: Muto
authors.forename: S
authors.initials: S
authors.lastname: Yokoyama
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D000926:Anticodon; D004926:Escherichia coli / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D012343:RNA, Transfer / Q000378:metabolism*
chemical_list: D000926:Anticodon; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases