title: [Establishment and characterization of a human cell line, HS-MM, derived from a case of clear cell sarcoma].
abstract: The characteristics of a new human clear cell sarcoma (CCSa) cell line, HS-MM, established from the pleural effusion in a 39-year-old man with lung metastasis, have been morphologically studied in vitro and in vivo. HS-MM cells growing on a cover-slip were round or spindle in shape with round nuclei containing extremely prominent nucleoli. Heterotransplantation of the cells into nude mice was easily succeeded following tumor development. Light microscopically, HS-MM cells, both in vitro and in vivo, were positive for anti-S-100 protein and anti-melanoma specific antibodies with immunostain, but no melanin pigment was detected in them. Ultrastructurally, the cells had round euchromatin-rich nuclei with large nucleoli revealing conspicuous nucleolonema, and contained a few mitochondria, rough endoplasmic reticulum and lysosomal dense bodies, besides a large amount of glycogen, but no melanosome in their cytoplasm. HS-MM cells retained and fully expressed morphologically unique characteristics as a CCSa, compatible with amelanotic type of malignant melanoma also. This cell line, HS-MM, therefore, proves to be extremely useful for clinicopathological studies on a CCSa.
authors.affiliation: Second Department of Pathology, Kochi Medical School, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Sonobe
authors.forename: Y
authors.initials: Y
authors.lastname: Manabe
authors.forename: M
authors.initials: M
authors.lastname: Furihata
authors.forename: J
authors.initials: J
authors.lastname: Iwata
authors.forename: T
authors.initials: T
authors.lastname: Hikita
authors.forename: T
authors.initials: T
authors.lastname: Tanimoto
authors.forename: O
authors.initials: O
authors.lastname: Kiuna
authors.forename: T
authors.initials: T
authors.lastname: Oka
authors.forename: Y
authors.initials: Y
authors.lastname: Ohtsuki
authors.forename: H
authors.initials: H
authors.lastname: Mizobuchi
mesh_terms: D000328:Adult; D000818:Animals; D006801:Humans; D007621:Karyotyping; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D012509:Sarcoma / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D014407:Tumor Cells, Cultured