title: Tubulin binds specifically to the signal-transducing proteins, Gs alpha and Gi alpha 1.
abstract: Participation of cytoskeletal elements in regulation of hormonal response and responsiveness has been suggested by several laboratories. Addition of dimeric tubulin to rat cerebral cortex synaptic membranes causes stable inhibition of adenylyl cyclase, and the molecular basis for this effect appears to require a direct interaction between tubulin and G proteins. To test whether such tubulin-G protein interaction occurred, several purified G proteins were bound to nitrocellulose, and 125I-tubulin overlay studies were performed. 125I-Tubulin bound to the alpha subunits of Gs and Gil with high specificity and an apparent Kd of approximately 130 nM. Other G protein alpha subunits (alpha i2, alpha i3, alpha 0, and transducin) displayed a much lower affinity for tubulin, despite the much closer relationship of those proteins to alpha il than to alpha s. Association of beta gamma subunits with alpha il or alpha s did not alter the binding of tubulin to these G protein heterotrimers, and the binding of a hydrolysis-resistant GTP analog to the alpha subunits was similarly without effect. These results suggest that tubulin forms complexes with specific G proteins and these complexes might provide a locus for the interaction of cytoskeletal components and signal transduction cascades. These results also provide evidence of a functional distinction among the closely related alpha i subtypes.
authors.affiliation: Department of Physiology and Biophysics, University of Illinois College of Medicine, Chicago 60680-6998.
authors.forename: N
authors.initials: N
authors.lastname: Wang
authors.forename: K
authors.initials: K
authors.lastname: Yan
authors.forename: M M
authors.initials: MM
authors.lastname: Rasenick
mesh_terms: D000818:Animals; D002540:Cerebral Cortex; D019204:GTP-Binding Proteins / Q000378:metabolism*; D046911:Macromolecular Substances; D011233:Precipitin Tests; D011485:Protein Binding; D051381:Rats; D013570:Synaptic Membranes / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism*
chemical_list: D046911:Macromolecular Substances; D014404:Tubulin; D019204:GTP-Binding Proteins