Towards a physical map of the Drosophila melanogaster genome: mapping of cosmid clones within defined genomic divisions.
A physical map of the D. melanogaster genome is being constructed, in the form of overlapping cosmid clones that are assigned to specific polytene chromosome sites. A master library of ca. 20,000 cosmids is screened with probes that correspond to numbered chromosomal divisions (ca. 1% of the genome); these probes are prepared by microdissection and PCR-amplification of individual chromosomes. The 120 to 250 cosmids selected by each probe are fingerprinted by Hinfl digestion and gel electrophoresis, and overlaps are detected by computer analysis of the fingerprints, permitting us to assemble sets of contiguous clones (contigs). Selected cosmids, both from contigs and unattached, are then localized by in situ hybridization to polytene chromosomes. Crosshybridization analysis using end probes links some contigs, and hybridization to previously cloned genes relates the physical to the genetic map. This approach has been used to construct a physical map of the 3.8 megabase DNA in the three distal divisions of the x chromosome. The map is represented by 181 canonical cosmids, of which 108 clones in contigs and 32 unattached clones have been mapped individually by in situ hybridization to chromosomes. Our current database of in situ hybridization results also includes the beginning of a physical map for the rest of the genome: 162 cosmids have been assigned by in situ hybridization to 129 chromosomal subdivisions elsewhere in the genome, representing 5 to 6 megabases of additional mapped DNA.
Sidén-Kiamos
I
I
Institute of Molecular Biology and Biotechnology, Research Center of Crete, Greece.
Saunders
R D
RD
Spanos
L
L
Majerus
T
T
Treanear
J
J
Savakis
C
C
Louis
C
C
Glover
D M
DM
Ashburner
M
M
Kafatos
F C
FC
D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular / Q000379:methods; D003360:Cosmids; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004331:Drosophila melanogaster / Q000235:genetics*; D005784:Gene Amplification; D015698:Genomic Library; D009693:Nucleic Acid Hybridization; D012984:Software
D004247:DNA