Replica plating of colonies from Listeria-selective agars to blood agar to improve the isolation of Listeria monocytogenes from foods.
Bacterial colonies from Listeria-selective agars were replica plated to sheep blood agar to screen for beta-hemolysis. By using the replica plating method to test for the beta-hemolytic characteristic of all the colonies growing on Listeria-selective agars instead of picking 3 to 10 suspected colonies for further testing, we recovered Listeria monocytogenes from 59 of 142 Listeria-selective agar plates which contained colonies of hemolytic and nonhemolytic Listeria species and were negative when tested by conventional colony picks.
Cassiday
P K
PK
Division of Bacterial Diseases, Centers for Disease Control, Atlanta, Georgia.
Graves
L M
LM
Swaminathan
B
B
D000362:Agar; D001431:Bacteriological Techniques; D005069:Evaluation Studies as Topic; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000209:etiology; D006801:Humans; D008089:Listeria monocytogenes / Q000254:growth & development / Q000302:isolation & purification*; D008088:Listeriosis / Q000209:etiology
D000362:Agar