The cyanelle genome of Cyanophora paradoxa, unlike the chloroplast genome, codes for the ribosomal L3 protein.
We describe a 1132 bp sequence of the cyanelle genome of Cyanophora paradoxa containing the rpl3 gene. This gene, which is not chloroplast encoded in plants, is the first of a long cyanelle ribosomal operon whose organization resembles that of the S10 operon of E. coli. We have shown that the rpl3 gene is transcribed in cyanelles as a 7500 nucleotide precursor and that the 5'-end of the mRNA starts approximately 90 nucleotides upstream from the initiation codon. However, no typical procaryotic promoter could be found for this gene. We have detected, using anti E. coli L3 antibodies, the cyanelle L3 protein in cyanelle extracts and in E. coli cells transformed with the cyanelle rpl3 gene.
Evrard
J L
JL
Institut de Biologie Moléculaire des Plantes, Université Louis Pasteur, Strasbourg, France.
Johnson
C
C
Janssen
I
I
Löffelhardt
W
W
Weil
J H
JH
Kuntz
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002736:Chloroplasts / Q000378:metabolism; D056890:Eukaryota / Q000235:genetics*; D005796:Genes; D008969:Molecular Sequence Data; D005810:Multigene Family; D009690:Nucleic Acid Conformation; D009876:Operon; D015388:Organelles / Q000378:metabolism*; D015183:Restriction Mapping; D000090822:Ribosomal Protein L3; D012269:Ribosomal Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D000090822:Ribosomal Protein L3; D012269:Ribosomal Proteins