Diode array detection of low level co-eluting species in high-performance liquid chromatography.
A simple and sensitive method for detecting low levels (0.5 and 1.0%, w/w) of co-eluting species in HPLC has been developed. This method is based on the subtraction of normalized peak up-slope and down-slope spectra from that of the apex. Visual inspection of the resultant "difference spectra" allows for a qualitative judgement regarding the integrity of the peak under consideration.
Anderson
N H
NH
Sterling Research Group, Alnwick, Northumberland, UK.
Gray
M R
MR
Hinds
C J
CJ
D000865:Antazoline / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003613:Danazol / Q000032:analysis; D005938:Glucocorticoids / Q000032:analysis; D007202:Indicators and Reagents; D013056:Spectrophotometry, Ultraviolet
D005938:Glucocorticoids; D007202:Indicators and Reagents; D000865:Antazoline; D003613:Danazol