title: Recombinant murine interferon-gamma inhibits the fusion of mouse alveolar macrophages in vitro but stimulates the formation of osteoclastlike cells on implanted syngeneic bone particles in mice in vivo.
abstract: Osteoclasts are multinucleated cells that originate from the fusion of mononuclear precursors and are responsible for bone resorption. Indirect evidence from in vitro studies suggests that IFN-gamma and TNF-alpha inhibit and stimulate bone resorption, respectively, but contradictory results have emerged from the literature regarding the effects of IFN-gamma on macrophage multinucleation. Using highly sensitive model systems, the present work demonstrates that, in mice, rMuIFN-gamma inhibits the fusion of alveolar macrophages in vitro but augments the number of osteoclastlike cells on implanted syngeneic bone particles in vivo. Although rMuTNF-alpha fails to stimulate macrophage multinucleation in either system, treatment of implanted animals with rMuIFN-gamma appears to limit the inflammatory reaction and favor tissue repair.
authors.affiliation: Department of Orthopedics, Yale University School of Medicine, New Haven, CT.
authors.forename: A
authors.initials: A
authors.lastname: Vignery
authors.forename: T
authors.initials: T
authors.lastname: Niven-Fairchild
authors.forename: M H
authors.initials: MH
authors.lastname: Shepard
mesh_terms: D000818:Animals; D001790:Blood Physiological Phenomena; D016025:Bone Transplantation / Q000473:pathology*; D002459:Cell Fusion / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D010010:Osteoclasts / Q000187:drug effects*; D011650:Pulmonary Alveoli / Q000166:cytology / Q000187:drug effects; D011994:Recombinant Proteins
chemical_list: D011994:Recombinant Proteins; D007371:Interferon-gamma