Serum autoantibodies in type 1 (insulin-dependent) diabetes mellitus: identification of reactivity against a 29 kd pancreas-specific protein.
Serum samples from patients with type 1 (insulin-dependent) diabetes mellitus and controls were incubated with two-dimensional Western immunoblots of pancreas and other tissues. Two out of 26 (8%) of the diabetics and 0 out of 45 of the controls demonstrated reactivity against four pancreas-specific proteins with identical molecular weights of 29,000 daltons and different isoelectric points ranging from pH 7.0-8.0. It is concluded that 29 Kd autoantibody reactivity is not a major marker for type 1 diabetes, but may help identify a subgroup of type 1 diabetics.
Frazier
J S
JS
Laboratory of Clinical Science, National Institute of Mental Health, Bethesda, Maryland 20892.
Oates
E L
EL
Salata
K F
KF
Jacobowitz
D M
DM
D000293:Adolescent; D001323:Autoantibodies / Q000097:blood*; D015153:Blotting, Western; D002648:Child; D002675:Child, Preschool; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008297:Male; D008970:Molecular Weight; D010179:Pancreas / Q000737:chemistry*; D011506:Proteins / Q000276:immunology*
D001323:Autoantibodies; D011506:Proteins