Identification of the Pseudomonas aeruginosa acid phosphatase as a phosphorylcholine phosphatase activity.
Choline, betaine and N,N-dimethylglycine as the sole carbon and nitrogen source induced a periplasmic acid phosphatase activity in Pseudomonas aeruginosa. This enzyme produced the highest rates of hydrolysis in phosphorylcholine and phosphorylethanolamine among the various phosphoric esters tested. At saturating concentrations of Mg2+, the Km values were 0.2 and 0.7 mM for phosphorylcholine and phosphorylethanolamine respectively. At high concentrations both compounds were inhibitors of the enzyme activity. The Ksi values for phosphorylcholine and phosphorylethanolamine were 1.0 and 3.0 mM respectively. The higher catalytic efficiency was that of phosphorylcholine. Considering these results it is possible to suggest that the Pseudomonas aeruginosa acid phosphatase is a phosphorylcholine phosphatase. The existence of this activity which is induced jointly with phospholipase C by different choline metabolites, in a high phosphate medium, suggests that the attack of Pseudomonas aeruginosa on the cell host may also be produced under conditions of high phosphate concentrations, when the alkaline phosphatase is absent.
Garrido
M N
MN
Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
Lisa
T A
TA
Albelo
S
S
Lucchesi
G I
GI
Domenech
C E
CE
D000135:Acid Phosphatase / Q000378:metabolism*; D002802:Cholinesterases / Q000378:metabolism; D004983:Ethanolamines / Q000378:metabolism; D007700:Kinetics; D009596:Nitrophenols / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D010767:Phosphorylcholine / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000254:growth & development; D013379:Substrate Specificity
D004983:Ethanolamines; D009596:Nitrophenols; D009943:Organophosphorus Compounds; D010767:Phosphorylcholine; C008644:nitrophenylphosphate; C005448:phosphorylethanolamine; D002802:Cholinesterases; D000135:Acid Phosphatase