Characterisation of a monoclonal antibody against native human type I collagen.
A monoclonal antibody against a pepsin-soluble mammalian type I collagen has been produced. This antibody, subclass IgG1, kappa, was specific for type I collagen and did not cross-react with a range of other collagen types or connective tissue proteins. The epitope recognized by the antibody was dependent upon an intact triple-helical structure for the collagen, and was shown by rotary shadowing and by immunoblotting of collagenase-derived fragments to be near the C-terminal of the pepsin-soluble collagen. Although the antibody had a low affinity, with Kd = 4 x 10(-7) M, it could be used for immunohistology of tissue sections and for studies of collagen produced by cells in culture. The antibody, which was raised against human collagen, also recognized type I collagens from certain other species, including calf, pig, sheep, goat and dog.
Werkmeister
J A
JA
Commonwealth Scientific and Industrial Research Organization, Division of Biotechnology, Parkville, Australia.
Ramshaw
J A
JA
Ellender
G
G
D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000096:biosynthesis; D000915:Antibody Affinity; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex / Q000032:analysis; D001666:Binding Sites, Antibody; D003094:Collagen / Q000032:analysis / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D006825:Hybridomas / Q000276:immunology / Q000378:metabolism; D010434:Pepsin A; D012995:Solubility
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D003094:Collagen; D010434:Pepsin A