title: Variations during leaf development of the relative amounts of two bean (Phaseolus vulgaris) chloroplast tRNAs(Phe) which differ in their minor nucleotide content.
abstract: Bean (Phaseolus vulgaris cv. Saxa) chloroplasts contain two tRNA(Phe) species, namely tRNA(Phe)1 and tRNA(Phe)2. By sequence determination, we show that tRNA(Phe)2 is identical to the previously sequenced tRNA(Phe)1 except for two undermodified nucleotides. By reversed-phase chromatography analyses, we demonstrate that the relative amounts of these two chloroplast tRNAs(Phe) vary during leaf development: in etiolated leaves the undermodified tRNA(Phe)2 only represents 15% of total chloroplast tRNA(Phe), during development and greening it increases to reach 60% in 8-day-old leaves, and it then decreases to 9% in senescing leaves.
authors.affiliation: Institut de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, Strasbourg, France.
authors.forename: H
authors.initials: H
authors.lastname: Pfitzinger
authors.forename: L
authors.initials: L
authors.lastname: Maréchal-Drouard
authors.forename: D T
authors.initials: DT
authors.lastname: Pillay
authors.forename: J H
authors.initials: JH
authors.lastname: Weil
authors.forename: P
authors.initials: P
authors.lastname: Guillemaut
mesh_terms: D001483:Base Sequence; D002736:Chloroplasts / Q000378:metabolism; D007887:Fabaceae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D063245:Plant Development; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D010946:Plants, Medicinal; D012323:RNA Processing, Post-Transcriptional; D012360:RNA, Transfer, Phe / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012265:Ribonucleotides / Q000032:analysis
chemical_list: D012360:RNA, Transfer, Phe; D012265:Ribonucleotides