Measurement of 5-methyltetrahydrofolic acid in man by high-performance liquid chromatography.
5-Methyl-5,6,7,8-tetrahydrofolic (5-methylTHF) acid can be measured in human serum and whole blood by fluorescence after deproteinization with perchloric acid and separation by high-performance liquid chromatography (HPLC). The technique is sensitive, rapid, and correlates with existing methods for total folates.
Leeming
R J
RJ
Haematology Department, General Hospital, Birmingham B4, England.
Pollock
A
A
Melville
L J
LJ
Hamon
C G
CG
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007202:Indicators and Reagents; D007780:Lacticaseibacillus casei / Q000187:drug effects / Q000254:growth & development; D013050:Spectrometry, Fluorescence; D013763:Tetrahydrofolates / Q000097:blood* / Q000494:pharmacology
D007202:Indicators and Reagents; D013763:Tetrahydrofolates; C005984:5-methyltetrahydrofolate