Protein TAU variants present in paired helical filaments (PHFs) of Alzheimer brains.
Polyclonal anti-TAU antisera directed against native Tau protein and the NH2-terminal side of the mouse TAU sequence were used to determine the nature of the TAU variants present in Alzheimer brains and in PHFs. These antibodies labelled specifically neurofibrillary tangles and plaque neurites in Alzheimer brains. On immunoblots of PHF extracts, two entities of 69 and 130 kDa were identified. These TAU-related species were absent from control brains. Protein immunoblot of total Alzheimer and control supernatants were shown to contain the same 4-5 TAU variants but none of the 69 and 130 TAU-related entities found in PHFs. These data suggest that specific TAU species are present in PHFs.
Gache
Y
Y
INSERM U282-CNRS, Hôpital Henri Mondor, Créteil, France.
Ricolfi
F
F
Guilleminot
J
J
Theiss
G
G
Nunez
J
J
D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology; D015153:Blotting, Western; D001921:Brain / Q000473:pathology; D001923:Brain Chemistry; D006624:Hippocampus / Q000737:chemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D008869:Microtubule-Associated Proteins / Q000032:analysis*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009454:Neurofibrils / Q000737:chemistry*; D014018:Tissue Distribution; D016875:tau Proteins
D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D016875:tau Proteins