title: Interleukin-1 beta and tumor necrosis factor-alpha synergistically stimulate nerve growth factor (NGF) release from cultured rat astrocytes.
abstract: Nerve growth factor (NGF) may mediate responses to brain injury. To examine regulation of NGF gene expression with respect to neural trauma we examined the effects of interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) on NGF production in cultures of rat astroglial cells. Purified neocortical astrocytes in serum-free medium were treated with IL-1 beta, TNF-alpha or both. Whereas IL-1 beta and TNF-alpha alone elicited only small effects, simultaneous addition elicited within 48 h a large (3- to 6-fold) increase in NGF content in culture supernatants. Our data are consistent with a role for cytokines in NGF synthesis and release in the injured central nervous system (CNS).
authors.affiliation: Department of Physiology, University of Basel, Switzerland.
authors.forename: R A
authors.initials: RA
authors.lastname: Gadient
authors.forename: K C
authors.initials: KC
authors.lastname: Cron
authors.forename: U
authors.initials: U
authors.lastname: Otten
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism; D004357:Drug Synergism; D007375:Interleukin-1 / Q000494:pharmacology*; D009414:Nerve Growth Factors / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D007375:Interleukin-1; D009414:Nerve Growth Factors; D014409:Tumor Necrosis Factor-alpha