title: The Adh gene promoters of Drosophila melanogaster and Drosophila orena are functionally conserved and share features of sequence structure and nuclease-protected sites.
abstract: The sibling species Drosophila melanogaster and D. orena show similar patterns of alcohol dehydrogenase expression, both spatially and temporally. These two species diverged from a common ancestor 6 million to 15 million years ago, and the DNA sequences of the promoter regions of their Adh genes show a mosaic pattern of conservation and change. By interspecific transformation of D. orena sequences into D. melanogaster, we demonstrate a functional equivalence between these sequences. Using both D. melanogaster embryo extracts and purified transcription factor Adf-1, we compare the protection of these promoter sequences from nuclease, demonstrating considerable conservation.
authors.affiliation: Howard Hughes Medical Institute Research Laboratories, University of California, Berkeley 94720.
authors.forename: K
authors.initials: K
authors.lastname: Moses
authors.forename: U
authors.initials: U
authors.lastname: Heberlein
authors.forename: M
authors.initials: M
authors.lastname: Ashburner
mesh_terms: D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D003850:Deoxyribonuclease I / Q000378:metabolism; D004330:Drosophila / Q000201:enzymology / Q000235:genetics*; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D006651:Histocytochemistry; D007814:Larva; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013379:Substrate Specificity; D014170:Transformation, Genetic
chemical_list: D000426:Alcohol Dehydrogenase; D003850:Deoxyribonuclease I