ADP-ribosylation of nuclear proteins in rat ventral prostate during ageing.
Poly(ADPR)polymerase activity and poly(ADP-ribosyl)ation of nuclear proteins have been investigated in ventral prostate nuclei of different aged rats (14, 28, 60, 180, 360 day old animals), by reverse-phase HPLC and acetic acid-urea polyacrylamide gel electrophoresis. The major ADP-ribose acceptor proteins were identified as histone H1 and H2b. It is concluded that concomitant with major changes to chromatin organization, poly(ADP-ribosyl)ation reaction is progressively inhibited during aging of rat ventral prostate. These results support the hypothesis that prostatic dysfunction in senescent animals is related to a failure of DNA repair mechanisms and deregulated template activity.
Quesada
P
P
Dipartimento di Chimica Organica e Biologica, Università di Napoli, Italy.
Faraone-Mennella
M R
MR
Jones
R
R
Malanga
M
M
Farina
B
B
D000375:Aging; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D002843:Chromatin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004260:DNA Repair; D008297:Male; D009687:Nuclear Proteins / Q000378:metabolism*; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism*; D011467:Prostate / Q000201:enzymology* / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains
D000596:Amino Acids; D002843:Chromatin; D009687:Nuclear Proteins; D011065:Poly(ADP-ribose) Polymerases