title: Essential role of ferrous iron in cyanide-resistant respiration in Hansenula anomala.
abstract: Antimycin A-dependent induction of cyanide-resistant respiration in Hansenula anomala was completely blocked by o-phenanthroline, alpha,alpha'-dipyridyl, or 8-hydroxyquinoline. Pulse-labeling of the cells with [35S]methionine in the presence of both antimycin A and o-phenanthroline indicated that the 36-kDa protein previously reported to be involved in cyanide-resistant respiration [(1989) J. Biochem. 105, 864-866] was formed in mitochondria even under these conditions. The addition of Fe2+, but not Fe3+, ions to these cells in the presence of cycloheximide resulted in the rapid expression of cyanide-resistant respiration activity. These results suggest that in the presence of both antimycin A and o-phenanthroline an inactive form of the 36-kDa protein was formed and Fe2+ ions converted it to the active form. It is also likely that Fe2+ ions are involved in the reaction mechanism of cyanide-resistant respiration.
authors.affiliation: Department of Biochemistry, Niigata College of Pharmacy, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Minagawa
authors.forename: S
authors.initials: S
authors.lastname: Sakajo
authors.forename: T
authors.initials: T
authors.lastname: Komiyama
authors.forename: A
authors.initials: A
authors.lastname: Yoshimoto
mesh_terms: D015082:2,2'-Dipyridyl / Q000494:pharmacology; D000968:Antimycin A / Q000494:pharmacology; D003486:Cyanides / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D005290:Ferric Compounds / Q000494:pharmacology; D005296:Ferrous Compounds / Q000494:pharmacology*; D008715:Methionine / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D015125:Oxyquinoline / Q000494:pharmacology; D010618:Phenanthrolines / Q000494:pharmacology; D010843:Pichia / Q000187:drug effects* / Q000378:metabolism; D004718:Saccharomycetales / Q000187:drug effects*
chemical_list: D003486:Cyanides; D005290:Ferric Compounds; D005296:Ferrous Compounds; D010618:Phenanthrolines; D015082:2,2'-Dipyridyl; D015125:Oxyquinoline; D000968:Antimycin A; D008715:Methionine; D010100:Oxygen; C025205:1,10-phenanthroline