Identification and characterization of the Egr-1 gene product, a DNA-binding zinc finger protein induced by differentiation and growth signals.
Egr-1 is an immediate-early response gene induced by diverse signals that initiate growth and differentiation. Its cDNA sequence predicts a protein with zinc fingers. We have generated an antiserum to the Egr-1 gene product and identified it as an 80-kilodalton short-lived protein in serum-stimulated mouse fibroblasts. The rat Egr-1 product has also been identified in nerve growth factor-induced PC12 cells. In addition, we show by cell fractionation and immunocytochemistry that the Egr-1 protein is located in the nucleus. We also demonstrate that it is phosphorylated. In vitro-generated Egr-1 protein binds with high affinity to the sequence CGCCCCCGC in a zinc-dependent manner.
Cao
X M
XM
Department of Medicine, University of Chicago, Illinois 60637.
Koski
R A
RA
Gashler
A
A
McKiernan
M
M
Morris
C F
CF
Gaffney
R
R
Hay
R V
RV
Sukhatme
V P
VP
D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002455:Cell Division; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D007158:Immunologic Techniques; D008667:Metalloproteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000494:pharmacology; D009687:Nuclear Proteins / Q000502:physiology*; D010750:Phosphoproteins / Q000235:genetics; D011499:Protein Processing, Post-Translational; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics*; D015032:Zinc / Q000502:physiology
D004268:DNA-Binding Proteins; D008667:Metalloproteins; D009414:Nerve Growth Factors; D009687:Nuclear Proteins; D010750:Phosphoproteins; D014157:Transcription Factors; D004815:Epidermal Growth Factor; D015032:Zinc; D013755:Tetradecanoylphorbol Acetate