[Demonstration of LH inhibitor(s) in sheep and human sera].
In mature rat Leydig cells, the testosterone output (24 ng/10(6) Leydig cells/4hrs.) is increased 10 fold by LH; the addition of serum from either control or castrated or hypophysectomized rams inhibits (60%) the LH-stimulated testosterone production. Similarly, the incubation of immature rat Leydig cells with sera from hypophysectomized patients leads to a diminution (70 and 30% respectively) of both basal (0.98 ng) and LH stimulated (3.44 ng) testosterone biosynthesis. These data suggest the existence of an LH inhibitor (or inhibitors) in blood from ram and human; in addition, this substance is not only of testicular origin and is not an LH-related molecule.
Boujrad
N
N
I.N.R.A., Nouzilly.
Denis
I
I
Papadopoulos
V
V
Pisselet
C
C
Bouchard
P
P
Drosdowsky
M A
MA
Hochereau de Reviers
M T
MT
Carreau
S
S
D000818:Animals; D006801:Humans; D007016:Hypophysectomy; D007985:Leydig Cells / Q000378:metabolism; D007986:Luteinizing Hormone / Q000037:antagonists & inhibitors* / Q000097:blood / Q000302:isolation & purification / Q000494:pharmacology; D008297:Male; D051381:Rats; D012756:Sheep / Q000097:blood*; D013739:Testosterone / Q000096:biosynthesis
D013739:Testosterone; D007986:Luteinizing Hormone
Mise en évidence d'un (ou des) inhibiteur(s) de LH dans les sérums ovin et humain.