Structure and expression of cysX, the second gene in the Escherichia coli K-12 cysE locus.
Protein products of the cysE region at 81 min on the chromosome of Escherichia coli K-12 (1) were analyzed by the maxicell method. Two kinds of polypeptides of molecular weight 33 K and 16 K were the products. The open reading frame (ORF) of the 33 K polypeptide consisted of 273 amino acids (Mr = 29,261). On the other hand, the 16 K ORF was overlapped by the opposite 33 K ORF and specified an extremely basic protein of 130 amino acids (Mr = 15,233). The gene coding for the 16 K polypeptide was named cysX. The expression of cysE and cysX in vivo was confirmed further by constructions of the cysE'-'lacZ and cysX'-'lacZ hybrid genes.
Tei
H
H
Research Institute for Food Science, Kyoto University, Japan.
Murata
K
K
Kimura
A
A
D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D003062:Codon / Q000235:genetics; D003090:Coliphages / Q000235:genetics; D003545:Cysteine / Q000096:biosynthesis*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping; D051050:Serine O-Acetyltransferase; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D003062:Codon; D004269:DNA, Bacterial; D000123:Acetyltransferases; D051050:Serine O-Acetyltransferase; D001616:beta-Galactosidase; D003545:Cysteine