Suppression of experimental allergic encephalomyelitis by intraventricular administration of interferon-gamma in Lewis rats.
Experimental allergic encephalomyelitis (EAE) is an autoimmune inflammatory disease of the central nervous system (CNS) which causes paralysis. Several studies have reported the involvement of Ia antigen-expressing cells in the pathogenesis of EAE. Interferon-gamma (IFN-gamma) can induce Ia antigen expression on a wide range of cells. We examined the effect of IFN-gamma on EAE in Lewis rats. Systemically administered IFN-gamma did not change the disease course of EAE, whereas IFN-gamma applied locally into the ventricular system of the CNS resulted in complete suppression of clinical signs. Furthermore, we found that systemic administration of anti-IFN-gamma just prior to the onset of clinical symptoms resulted in a more severe disease course. We conclude that IFN-gamma is capable of exerting a suppressive action in EAE, possibly through induction of Ia antigen expression or through the induction of suppressive mechanisms locally in the CNS.
Voorthuis
J A
JA
Department of Cell Biology, Free University, Amsterdam, The Netherlands.
Uitdehaag
B M
BM
De Groot
C J
CJ
Goede
P H
PH
van der Meide
P H
PH
Dijkstra
C D
CD
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000627:therapeutic use; D002531:Cerebellum / Q000276:immunology / Q000473:pathology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000473:pathology / Q000517:prevention & control* / Q000628:therapy; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D007124:Immunoenzyme Techniques; D007274:Injections, Intraperitoneal; D007276:Injections, Intraventricular; D007371:Interferon-gamma / Q000008:administration & dosage / Q000276:immunology / Q000627:therapeutic use*; D008297:Male; D009900:Optic Nerve / Q000276:immunology / Q000473:pathology; D051381:Rats; D011917:Rats, Inbred Lew; D011994:Recombinant Proteins
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D011994:Recombinant Proteins; D007371:Interferon-gamma