XeCl laser action at medium fluences on biological tissues: fluorescence study and simulation with a chemical solution.
A rat heart, isolated and perfused, was irradiated with a XeCl excimer laser at 308 nm. The evolution of the fluorescence spectrum was measured. For an incident energy E greater than 4 kJ m-2 per pulse the fluorescence changed with time in a complex and spectrally non-uniform way. The proposed interpretation is that the radiation acts on the cellular respiratory chain. Buffered solutions of NADH, cytochrome c and FAD, which play a role in the respiratory chain, were irradiated in order to simulate the in vivo findings. The conclusion of this study is that XeCl radiation introduces a modification in the functioning of the respiratory chain: it accelerates electron transfer, but this quickly leads to an interruption of the respiratory chain.
Berthier
J P
JP
Laboratoire de Physique des Lasers, Université Paris XIII, Villetaneuse, France.
Raynal
E
E
Kimel
S
S
Avrillier
S
S
Ollivier
J P
JP
D000818:Animals; D002712:Chlorides; D007834:Lasers; D009206:Myocardium / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects; D010477:Perfusion; D051381:Rats; D012402:Rotenone / Q000494:pharmacology; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods; D014978:Xenon
D002712:Chlorides; D012402:Rotenone; D014978:Xenon; C048006:xenon chloride