[Possibility of use of electrophoresis on polyacrylamide gel with SDS in native human saliva].
The pooled mixture of mixed and parotid samples of human saliva were investigated. The contents of proteins used in gel were lower than 300 micrograms and higher than 300 micrograms, depending on electric power and used boiled water bath. We established that the method according to Laemmli requires the mixture of buffer samples in which the content of proteins is over 300 micrograms, boiled one minute and a half in water bath. The electrophoretic process was carried out with electric power of 1 mA per sample and reproducible results were obtained. This method can also be simply used in the examination of acid proteins and basic characteristic, which are of great importance in the maintenance of normal physiological position of oral tissues. This new method can be performed without previous concentration or liophylisation of saliva.
Mirković
S
S
University School of Dental Medicine, Belgrade.
D000328:Adult; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D012463:Saliva / Q000737:chemistry; D012471:Salivary Proteins and Peptides / Q000032:analysis*
D012471:Salivary Proteins and Peptides
Mogućnost primene elektroforeze na poliakrilamidnom gelu uz dodatak SDS na uzorke nativne humane pljuvacke.