title: Effects of passively immunizing ewes against inhibin and oestradiol during the follicular phase of the oestrous cycle.
abstract: Passive immunization was used to investigate the importance of inhibin and oestradiol in the control of FSH production during the follicular phase of the oestrous cycle in the sheep. Four groups of five mature Scottish Blackface ewes were injected with normal sheep plasma (control), antiserum to the 1-26 alpha peptide fragment of porcine inhibin, antiserum to oestradiol-17 beta, or a combination of the two antisera, 24 h following cloprostenol-induced luteal regression. There was no difference in the concentration of LH in jugular venous plasma between the control and inhibin-immunized groups following the injection of normal sheep plasma or inhibin antiserum, with both groups exhibiting normal LH surges. In both the groups immunized against oestradiol, the basal concentration of LH rose by 25-30% (P less than 0.05) during the 96-h period following injection, while the LH surge and consequent formation of a corpus luteum was inhibited. In all three immunized groups there was a significant (P less than 0.001) rise in the concentration of FSH starting 3.8-4.8 h after the injection of antiserum. The duration of the rise was similar in the groups injected with oestradiol antiserum alone (43.6 +/- 12.8 h) or in combination with inhibin antiserum (40.6 +/- 11.7 h), but was significantly (P less than 0.05) shorter in the group immunized against inhibin alone (17.0 +/- 0.5 h).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Obstetrics and Gynaecology, University of Edinburgh.
authors.forename: G E
authors.initials: GE
authors.lastname: Mann
authors.forename: B K
authors.initials: BK
authors.lastname: Campbell
authors.forename: A S
authors.initials: AS
authors.lastname: McNeilly
authors.forename: D T
authors.initials: DT
authors.lastname: Baird
mesh_terms: D000818:Animals; D003008:Cloprostenol / Q000494:pharmacology; D004958:Estradiol / Q000276:immunology / Q000502:physiology*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood*; D005498:Follicular Phase / Q000502:physiology; D007106:Immune Sera; D007116:Immunization, Passive / Q000379:methods*; D007265:Inhibins / Q000276:immunology / Q000502:physiology*; D007986:Luteinizing Hormone / Q000097:blood; D010060:Ovulation / Q000187:drug effects; D011374:Progesterone / Q000097:blood; D012756:Sheep / Q000502:physiology*
chemical_list: D007106:Immune Sera; D003008:Cloprostenol; D011374:Progesterone; D004958:Estradiol; D007265:Inhibins; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone