title: Discriminating between protective and enhancing HIV antibodies.
abstract: Most attempts to produce a vaccine against HIV-1 infection are utilizing envelope protein components. Hypothetically such vaccine candidates could stimulate production of antibodies that enhance HIV-1 infection via the macrophage route of entry and, consequently, cannot be detected in the conventional neutralization assay. To study this hypothesis we report an assay designed to evaluate the protective/enhancing activity of serum from seropositive immunized or infected individuals. Highly purified activated FcR-bearing monocytes-macrophages were infected with HIV-1 in the presence of the sera, then washed and cocultured with activated peripheral blood mononuclear cells (PBMC) from a normal donor. Productive viral infection, as evaluated by p24 antigen semiquantitative assay in the culture supernatants, allow evaluation of protective/enhancing activity of the sera. The data clearly show that protective rather than enhancing activity is present in the serum of env protein-immunized individuals.
authors.affiliation: Institut Jean-Godinot, Reims, France.
authors.forename: J
authors.initials: J
authors.lastname: Bernard
authors.forename: B
authors.initials: B
authors.lastname: Reveil
authors.forename: I
authors.initials: I
authors.lastname: Najman
authors.forename: F
authors.initials: F
authors.lastname: Liautaud-Roger
authors.forename: M
authors.initials: M
authors.lastname: Fouchard
authors.forename: O
authors.initials: O
authors.lastname: Picard
authors.forename: A
authors.initials: A
authors.lastname: Cattan
authors.forename: A
authors.initials: A
authors.lastname: Mabondzo
authors.forename: S
authors.initials: S
authors.lastname: Laverne
authors.forename: R C
authors.initials: RC
authors.lastname: Gallo
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D002478:Cells, Cultured; D015683:Gene Products, gag / Q000276:immunology*; D015483:HIV Antibodies / Q000276:immunology*; D015488:HIV Antigens / Q000276:immunology*; D016655:HIV Core Protein p24; D006679:HIV Seropositivity / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000382:microbiology; D008264:Macrophages / Q000382:microbiology; D009000:Monocytes / Q000382:microbiology; D011897:Random Allocation; D014758:Viral Core Proteins / Q000276:immunology*
chemical_list: D015683:Gene Products, gag; D015483:HIV Antibodies; D015488:HIV Antigens; D016655:HIV Core Protein p24; D014758:Viral Core Proteins