Dynamic fluorescence in copper proteins. Selected examples.
The fluorescence properties of three copper proteins, namely human superoxide dismutase, Pseudomonas aeruginosa azurin and Thiobacillus versutus amicyanin have been studied. All these proteins show a non-exponential decay of fluorescence, though the tryptophanyl residues responsible for the emission are very differently located in the three proteins. All the three decays can be fitted by at least two lifetimes or better with one or two lorentzian-shaped, continuous distributions of lifetime. In each case the removal of copper affects the quantum yield of fluorescence without affecting the shape of the emission.
Rosato
N
N
Department of Physics, University of Illinois, Urbana-Champaign.
Gratton
E
E
Mei
G
G
Savini
I
I
Finazzi Agrò
A
A
D001400:Azurin / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry; D003300:Copper / Q000737:chemistry*; D019791:Guanidine; D006146:Guanidines; D006801:Humans; D008667:Metalloproteins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013482:Superoxide Dismutase / Q000737:chemistry; D013696:Temperature; D014364:Tryptophan / Q000737:chemistry
D001426:Bacterial Proteins; D006146:Guanidines; D008667:Metalloproteins; C017079:mauC protein, Methylobacterium extorquens; D001400:Azurin; D003300:Copper; D014364:Tryptophan; D013482:Superoxide Dismutase; D019791:Guanidine