title: Isolation of two hemorrhagic toxins from Crotalus basiliscus basiliscus (Mexican west coast rattlesnake) venom and their effect on blood clotting and complement.
abstract: 1. Two hemorrhagic toxins of mol. wt 27,000 (B1) and 27,500 (B2) and pI 9.8 and 5.2 respectively were isolated from Crotalus basiliscus venom. 2. The two proteinases did not cross-react antigenically. 3. Both toxins caused hemorrhage in mice and each was capable of hydrolyzing hide power azure, casein, collagen and fibrin. 4. B1 hydrolyzed the A alpha, B beta and gamma chains of fibrinogen. B2 hydrolyzed the A alpha and B beta chains of fibrinogen, but not the gamma chain. 5. Both proteinases inactivated guinea pig complement.
authors.affiliation: Department of Biological Sciences, University of Texas, El Paso 79968.
authors.forename: O
authors.initials: O
authors.lastname: Molina
authors.forename: R K
authors.initials: RK
authors.lastname: Seriel
authors.forename: M
authors.initials: M
authors.lastname: Martinez
authors.forename: M L
authors.initials: ML
authors.lastname: Sierra
authors.forename: A
authors.initials: A
authors.lastname: Varela-Ramirez
authors.forename: E D
authors.initials: ED
authors.lastname: Rael
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D002848:Chromatography, DEAE-Cellulose; D002863:Chromogenic Compounds / Q000494:pharmacology; D003165:Complement System Proteins / Q000187:drug effects*; D003435:Crotalid Venoms / Q000032:analysis*; D004492:Edetic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000302:isolation & purification* / Q000502:physiology; D005340:Fibrinogen / Q000378:metabolism; D005343:Fibrinolytic Agents / Q000494:pharmacology; D006470:Hemorrhage / Q000139:chemically induced*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D014118:Toxins, Biological / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D002863:Chromogenic Compounds; D003435:Crotalid Venoms; D005343:Fibrinolytic Agents; D014118:Toxins, Biological; D005340:Fibrinogen; D003165:Complement System Proteins; D004492:Edetic Acid; C019724:arginine esterase; D002265:Carboxylic Ester Hydrolases; D010450:Endopeptidases