Biogenesis of chromomycin A3 by Streptomyces griseus.
The biosynthesis of chromomycin A3 was investigated using 13C-labeled acetates, methionine and glucose, and 13C,18O-labeled acetate. 13C NMR spectral analysis demonstrated that: Aglycone assembly occurs by combining at least two polyketide chains; three of nine oxygen atoms of the aglycone originate from acetate precursor oxygen atoms; carbon methylations on the aromatic ring at C-7, on the chromose B sugar, and two O-methylations appear to be carried out by S-adenosyl-methionine requiring methyl transferases; and glucose is the precursor for all the sugars.
Montanari
A
A
Biocatalysis Research Group, College of Pharmacy, University of Iowa, Iowa City 52242.
Rosazza
J P
JP
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D014128:Chromomycin A3 / Q000096:biosynthesis*; D002865:Chromomycins / Q000096:biosynthesis*; D005285:Fermentation; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D013056:Spectrophotometry, Ultraviolet; D013305:Streptomyces griseus / Q000378:metabolism*
D002865:Chromomycins; D014128:Chromomycin A3