Modification of a glnB-like gene product by photosynthetic electron transport in the cyanobacterium Synechococcus 6301.
Covalent modification of a 13 kDa soluble-phase protein occurs during adaptation of cells of the cyanobacterium Synechococcus 6301 (mutant AN112) to light specifically absorbed by photosystem II. This adaptation is accompanied by functional changes indicative of altered excitation energy distribution between the photosystems. The 13 kDa protein is identified by solid-phase N-terminal sequencing as a protein related to PII, the glnB gene product of E. coli. In E. coli, the PII protein undergoes uridylylation and acts as a regular of glutamine synthetase at both the post-translational and transcriptional levels. The implications of modification of a transcriptional regulator by photosynthetic electron transport are discussed.
Harrison
M A
MA
Department of Pure and Applied Biology, University of Leeds, UK.
Keen
J N
JN
Findlay
J B
JB
Allen
J F
JF
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D002734:Chlorophyll / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D000458:Cyanobacteria / Q000235:genetics / Q000378:metabolism*; D004579:Electron Transport; D004926:Escherichia coli / Q000235:genetics; D017343:Genes, Plant; D008027:Light; D045342:Light-Harvesting Protein Complexes; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009154:Mutation; D051922:PII Nitrogen Regulatory Proteins; D010710:Phosphates; D010766:Phosphorylation; D010788:Photosynthesis; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid
D001426:Bacterial Proteins; D045342:Light-Harvesting Protein Complexes; D051922:PII Nitrogen Regulatory Proteins; D010710:Phosphates; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D002734:Chlorophyll; C404143:PIID regulatory protein, Bacteria