Chemical synthesis of the 5'-terminal structure of U1 RNA.
A new method for the synthesis of N2,N2-dimethylguanosine (DMG) is developed by reductive C-S bond cleavage by use of tributyltin hydride. An improved method for the synthesis of the key intermediate (1) for construction of the 5'-terminal structure of U1 RNA, which has a trimethylated cap (TMG) structure at its 5' end, is also described. By the use of 1, several TMG-capped ribonucleosides and oligonucleotides were synthesized.
Sekine
M
M
Department of Life Chemistry, Tokyo Institute of Technology, Yokohama, Japan.
Satoh
T
T
D006151:Guanosine / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D006367:HeLa Cells; D006801:Humans; D015394:Molecular Structure; D012342:RNA, Small Nuclear / Q000737:chemistry*
D012342:RNA, Small Nuclear; D006151:Guanosine; C008940:N(2),N(2)-dimethylguanosine