Isolation and sequencing of rat liver bilirubin UDP-glucuronosyltransferase cDNA: possible alternate splicing of a common primary transcript.
A 1763-bp cDNA for rat liver bilirubin UDP-glucuronosyltransferase (UDPGT) was isolated. Bilirubin UDPGT activity was demonstrated by transfection of the pcDL1 vector carrying the cDNA into COS7 monkey kidney cells. The cDNA shares an identical 913-bp sequence (corresponding to the C-terminal 247 amino acid residues) with that for rat liver 3-methylcholanthrene-inducible 4-nitrophenol UDPGT including the locus where a -1 frameshift mutation was found in the 4-nitrophenol UDPGT cDNA from the jaundiced homozygous Gunn rat. The result suggests that both the UDPGTs are derived from a common primary-transcript and that the multiple defects of UDPGT isoenzymes observed in the homozygous Gunn rat may be produced by a single-mutated-locus after an alternative splicing of the 5' end region.
Sato
H
H
Department of Perinatology, Institute for Developmental Research, Aichi Prefecture Colony, Japan.
Koiwai
O
O
Tanabe
K
K
Kashiwamata
S
S
D000818:Animals; D001483:Base Sequence; D001663:Bilirubin / Q000378:metabolism; D002460:Cell Line; D004247:DNA / Q000302:isolation & purification*; D014453:Glucuronosyltransferase / Q000096:biosynthesis / Q000235:genetics*; D000882:Haplorhini; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D012326:RNA Splicing; D051381:Rats; D014162:Transfection
D004247:DNA; D014453:Glucuronosyltransferase; D001663:Bilirubin