The definition of five B lymphocyte alloantigens closely linked to BoLA class I antigens.
B lymphocyte alloantigens in cattle were identified by serological analysis. Alloantisera were raised by skin implant immunization or leucocyte immunization and were absorbed with platelets to reduce class I-specific antibody activity. Leucocyte absorptions were done to reduce the complexity of some antisera. A panning technique was used to prepare B-enriched and B-depleted lymphocytes. Antisera which displayed anti-B cell activity over a number of dilutions were tested against 115 Charolais cattle, and 13 antisera were used to define five B lymphocyte alloantigens. These antigens were present on B lymphocytes but did not appear to be present, at least at the same density, on the majority of T lymphocytes or platelets. Family studies suggested that these antigens are coded by one or two loci which are closely linked to the bovine class I loci. These results suggest the five antigens are class II antigens of the major histocompatibility complex (MHC) of cattle.
Mackie
J T
JT
Department of Immunology, John Curtin School of Medical Research, Australian National University, Canberra.
Stear
M J
MJ
D000818:Animals; D002417:Cattle / Q000235:genetics / Q000276:immunology*; D005260:Female; D005805:Genes, MHC Class I; D005802:Genes, MHC Class II; D008040:Genetic Linkage; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D007518:Isoantibodies; D008297:Male
D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D007518:Isoantibodies