title: Elongation of fetal chick long bone in vitro is formed by a mitogenic activity preparation from porcine bone.
abstract: Elongation of fetal chick long bone rudiments is formed by a mitogenic activity from porcine bone in vitro. Fractions of mitogenic activity from a heat- and acid-treated extract and from sequential chromatography on hydroxyapatite and from gel-filtration in 4 M guanidine-HCl increase diaphyseal elongation of metatarsals. The bone elongation-forming activity is associated with the mitogenic activity estimated by the incorporation of [3H]-methyl thymidine into the DNA of cells from embryonic chick periosteum. Histological examination of the mitogen-treated embryonic chick long bone shows that the partially purified fractions with a preferential effect on osteogenic cells increase diaphyseal elongation via cartilage cell proliferation.
authors.affiliation: Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, FRG.
authors.forename: K G
authors.initials: KG
authors.lastname: Kukoschke
authors.forename: G
authors.initials: G
authors.lastname: Delling
authors.forename: H
authors.initials: H
authors.lastname: Mayer
mesh_terms: D000818:Animals; D001846:Bone Development / Q000187:drug effects*; D001842:Bone and Bones / Q000032:analysis / Q000166:cytology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D002850:Chromatography, Gel / Q000379:methods; D008934:Mitogens / Q000032:analysis / Q000494:pharmacology*; D013552:Swine
chemical_list: D008934:Mitogens