title: Characterization of a putative promoter region of the human poly(ADP-ribose) polymerase gene: structural similarity to that of the DNA polymerase beta gene.
abstract: The 5'-flanking region of the human poly(ADP-ribose) polymerase gene was isolated and characterized. The nucleotide sequence of a part of the poly(ADP-ribose) polymerase gene completely matched that of the cDNA. The transcriptional initiation sites (cap sites) of this gene, located about 166-bp upstream from the translational initiation site, were identified by S1 mapping analysis. Neither CAAT box nor TATA box was found within 500-bp upstream from the cap sites of poly(ADP-ribose) polymerase gene. The 200-bp immediately upstream of the cap site had a high G+C content (76.5%) and contained double repeats of the sequence CCGCCC, putative Sp1 binding sites, and a palindromic structure. The 5'-flanking region of poly(ADP-ribose) polymerase gene also showed promoter activity in chloramphenicol acetyltransferase assay and structural similarity to that of DNA polymerase beta gene.
authors.affiliation: Biochemistry Division, National Cancer Center Research Institute, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ogura
authors.forename: H
authors.initials: H
authors.lastname: Nyunoya
authors.forename: M
authors.initials: M
authors.lastname: Takahashi-Masutani
authors.forename: M
authors.initials: M
authors.lastname: Miwa
authors.forename: T
authors.initials: T
authors.lastname: Sugimura
authors.forename: H
authors.initials: H
authors.lastname: Esumi
mesh_terms: D001483:Base Sequence; D003360:Cosmids; D004247:DNA / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D004256:DNA Polymerase I / Q000235:genetics*; D005796:Genes; D015698:Genomic Library; D006801:Humans; D007438:Introns; D007962:Leukocytes / Q000201:enzymology; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D011065:Poly(ADP-ribose) Polymerases / Q000097:blood / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D004247:DNA; D011065:Poly(ADP-ribose) Polymerases; D004256:DNA Polymerase I