Fructose formation in stored blood.
At the high glucose concentrations used in the collection and storage of donor blood the activity of the fructose-forming polyol pathway (Reaction I and II) could act to deplete NADPH and thus GSH, thereby exposing the cells to oxidative stress. Fructose levels were found to be high in red cells and the supernatant plasma of blood collected into CP2D, which contains 258 mM glucose. Elevated fructose was not produced by the polyol pathway, but was formed by the autoclaving process. A high fructose concentration sufficient to account for the fructose in donor red cells was also found in the CP2D anticoagulant and in samples of autoclaved glucose.
Guppy
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Biochemistry Department, University of W.A., Nedlands, Australia.
Sabaratnam
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Devadason
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Whisson
M E
ME
D001782:Blood Donors; D001800:Blood Specimen Collection; D004492:Edetic Acid; D004912:Erythrocytes / Q000378:metabolism*; D005632:Fructose / Q000096:biosynthesis / Q000097:blood*; D005947:Glucose / Q000378:metabolism; D006801:Humans; D013997:Time Factors
D005632:Fructose; D004492:Edetic Acid; D005947:Glucose