Aphidicolin-resistant DNA polymerase of bacteriophage phi 29 APHr71 mutant is hypersensitive to phosphonoacetic acid and butylphenyldeoxyguanosine 5'-triphosphate.
Bacteriophage phi 29 DNA polymerase is sensitive to aphidicolin (APH). DNA polymerase of the APH-resistant mutant, APHr71, was more sensitive to phosphonoacetic acid and butylphenyldeoxyguanosine 5'triphosphate than the wild type. Nucleotide sequence analysis revealed a single transition of G at nucleotide 562 to A in the DNA polymerase gene of APHr71, indicating that APHr71 DNA polymerase (572 residues) had a single amino acid substitution from glycine at residue 188 to serine. The results suggest that the site and the neighboring conserved segment of phi 29 DNA polymerase constitute a structure interacting with deoxynucleotides, pyrophosphate, and APH.
Matsumoto
K
K
Life Science Institute, Sophia University, Tokyo, Japan.
Kim
C I
CI
Kobayashi
H
H
Kanehiro
H
H
Hirokawa
H
H
D000595:Amino Acid Sequence; D000998:Antiviral Agents / Q000494:pharmacology*; D016590:Aphidicolin; D001435:Bacteriophages / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D004257:DNA Polymerase II / Q000032:analysis*; D003848:Deoxyguanine Nucleotides / Q000494:pharmacology*; D004224:Diterpenes / Q000494:pharmacology*; D004351:Drug Resistance / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation; D010746:Phosphonoacetic Acid / Q000494:pharmacology*
D000998:Antiviral Agents; D003848:Deoxyguanine Nucleotides; D004224:Diterpenes; D016590:Aphidicolin; C040364:N(2)-(4-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate; D004257:DNA Polymerase II; D010746:Phosphonoacetic Acid