title: Phenylmethylsulfonyl fluoride protects L-lysine transport in Schizosaccharomyces pombe against inactivation by ammonium ions.
abstract: Ammonium ions inactivate the basic amino acid transport system in Schizosaccharomyces pombe in an irreversible manner. The inactivation is accompanied by a 4-fold decrease of KT of L-lysine transport, leaving its Jmax unchanged; phenylmethylsulfonyl fluoride protects the system against inactivation. In contrast, two basic amino acid transport systems in a gap1 mutant of Saccharomyces cerevisiae are influenced by NH4+ ions in such a way that only the Jmax decreases while the KT of L-lysine transport is unchanged. Phenylmethylsulfonyl fluoride does not act here as a protective agent.
authors.affiliation: Department of Membrane Transport, Czechoslovak Academy of Sciences, Prague.
authors.forename: J
authors.initials: J
authors.lastname: Horák
authors.forename: H
authors.initials: H
authors.lastname: Sychrová
authors.forename: A
authors.initials: A
authors.lastname: Kotyk
mesh_terms: D000641:Ammonia / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D008239:Lysine / Q000378:metabolism*; D009154:Mutation; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D004718:Saccharomycetales / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000187:drug effects / Q000378:metabolism*; D013450:Sulfones / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D013450:Sulfones; D010664:Phenylmethylsulfonyl Fluoride; D000641:Ammonia; D008239:Lysine