Urinary crystal surface binding substances on calcium oxalate crystals.
In order to study the effect of urinary crystal surface binding substances (CSBS), we extracted the naturally existing CSBS from urine from healthy individuals by conducting homogeneous crystallization of calcium oxalate. CSBS proved not to be promoters but rather strong inhibitors of calcium oxalate crystal growth and aggregation. It is suggested that CSBS exhibited their inhibitory effect by masking the growing sites and aggregating sites on the crystal surface. As for the characteristics of CSBS, we found around 10 peaks of molecular weight, and all of them contained both peptides and saccharides. The findings suggest that CSBS are composed of various kinds of glycoproteins and proteoglycans.
Koide
T
T
Department of Urology, Osaka University School of Medicine, Japan.
Yoshioka
T
T
Yamaguchi
S
S
Hosokawa
S
S
Utsunomiya
M
M
Sonoda
T
T
D000328:Adult; D002129:Calcium Oxalate / Q000037:antagonists & inhibitors* / Q000737:chemistry* / Q000302:isolation & purification; D002845:Chromatography; D003460:Crystallization; D004588:Electrophoresis, Cellulose Acetate; D008401:Gas Chromatography-Mass Spectrometry; D006023:Glycoproteins / Q000302:isolation & purification*; D006801:Humans; D046911:Macromolecular Substances; D008297:Male; D014545:Urinary Calculi / Q000737:chemistry / Q000652:urine; D014556:Urine / Q000737:chemistry*
D006023:Glycoproteins; D046911:Macromolecular Substances; C029490:nephrocalcin; D002129:Calcium Oxalate