title: The purification and properties of aldose reductase from rat ovary.
abstract: Aldose reductase has been highly purified from rat ovary to apparent homogeneity, as assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The purified enzyme proved to be a monomeric protein with a molecular weight of about 39,900. The enzyme catalyzed the NADPH-dependent reduction of a number of aromatic and aliphatic aldehydes as well as aldo-sugars. The enzyme was potently inhibited by p-chloro-mercuribenzoate and a commercially developed aldose reductase inhibitor, M79175. The result of an immunoinhibition study, using antibody against the purified enzyme, indicated that the enzyme was responsible for more than 50% of the overall catalytic activity of D-glucose reduction in rat ovarian cytosol. Western blotting analysis revealed that immunoreactive proteins to anti-ovarian aldose reductase antibody were present in adrenal gland, various reproductive tissues, brain, lung, and heart of rats. Furthermore, ovarian tissues of various species contained immunoreactive proteins, though in small amounts. The enzyme was primarily localized in the granulosa cells and oocytes of all stages of follicular development during the estrous cycle, though it was also found in the corpora lutea cells in the pregnant rats.
authors.affiliation: Department of Pharmacology and Toxicology, Tokyo College of Pharmacy, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Iwata
authors.forename: N
authors.initials: N
authors.lastname: Inazu
authors.forename: T
authors.initials: T
authors.lastname: Satoh
mesh_terms: D000449:Aldehyde Reductase / Q000302:isolation & purification / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D003600:Cytosol / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D004971:Estrus; D005260:Female; D007150:Immunohistochemistry; D007700:Kinetics; D046911:Macromolecular Substances; D008970:Molecular Weight; D010053:Ovary / Q000166:cytology / Q000201:enzymology*; D011247:Pregnancy; D051381:Rats; D011921:Rats, Inbred WKY; D013379:Substrate Specificity; D014462:Ultrafiltration
chemical_list: D000596:Amino Acids; D046911:Macromolecular Substances; D000449:Aldehyde Reductase