[Effect of humoral factors produced by lymphocytes on hematopoietic progenitor cells--productive ability of colony stimulating activities and interferon-gamma by blood mononuclear cells in patients with aplastic anemia].
To evaluate the role of cytokines in patients with aplastic anemia, colony stimulating activities (CSA) and interferon-gamma (IFN-gamma) in cultured media of lymphocytes with phytohemagglutinin (PHA-LCM) were measured with methylcellulose culture method in 20 patients (age 3 to 69 years). The CSA for granulocyte/macrophage (GM-CSA) in patients was equivalent to that of normal donors, while low burst promoting activity (BPA) was observed in PHA-LCM from 7 adult patients (61 +/- 17%). The ability of BPA production varied widely in 13 children (97 +/- 37%). In some patients, low production of BPA improved after successful treatment of antilymphocyte globulin. The IFN-gamma in PHA-LCM disclosed no significant difference between patients and normal donors. From these results, low production of BPA may have a role in the development of AA in certain patients. It is also suggested that therapy with recombinant cytokines such as GM-CSF and IL-3, detected as BPA in our culture system, could be effective for those patients.
Kawano
Y
Y
Department of Pediatrics, School of Medicine, University of Tokushima.
D000328:Adult; D000368:Aged; D000741:Anemia, Aplastic / Q000503:physiopathology* / Q000628:therapy; D000961:Antilymphocyte Serum / Q000627:therapeutic use; D002648:Child; D002675:Child, Preschool; D003115:Colony-Stimulating Factors / Q000378:metabolism*; D015672:Erythroid Precursor Cells / Q000502:physiology*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
D000961:Antilymphocyte Serum; D003115:Colony-Stimulating Factors; D007371:Interferon-gamma