Identification of DNA degradation products by antitumor antibiotic, esperamicin.
Esperamicin A1 is a DNA-damaging agent characterized by a unique ten-membered ene-diyne core. We studied the detailed reaction mechanism by using synthetic DNA oligomers. The cleavage site and activity depend on the sequence of the oligomers. d(GGATCC) and d(GGTACC) were cleaved by the drug while d(CCATGG) and d(CCTAGG) were not cleaved under the present conditions. d(GGTACC) gave two major 5'-fragments. The result of partial nuclease digestion experiments suggests that these products are trimer and pentamer with a modified 3'-end.
Ebata
T
T
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Hiroaki
H
H
Uesugi
S
S
Sugiura
Y
Y
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001483:Base Sequence; D004247:DNA / Q000187:drug effects*; D003854:Deoxyribonucleotides / Q000138:chemical synthesis; D053281:Enediynes
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D003854:Deoxyribonucleotides; D053281:Enediynes; D004247:DNA; C047543:esperamicin A1