title: Bovine dental pulp collagens: characterization of types III and V collagen.
abstract: Pulp was essentially solubilized by partial pepsin digestion. The various genetic types of collagens were isolated by differential salt precipitation and extraction. Types I, III and V collagen represented 56, 41 and 2% of the total collagen, respectively. The type V collagen comprised two different molecular species consisting of [alpha 1(V)]2 alpha 2(V) and alpha 1(V) alpha 2(V) alpha 3(V), the ratio of which was approx. 1:1.3. The major portion of the type III collagen was present as a high molecular weight aggregate which released alpha 1(III) chains upon reduction with 2-mercaptoethanol.
authors.affiliation: CB No. 7455 Dental Research Center, University of North Carolina, Chapel Hill 27599-7455.
authors.forename: M
authors.initials: M
authors.lastname: Tsuzaki
authors.forename: M
authors.initials: M
authors.lastname: Yamauchi
authors.forename: G L
authors.initials: GL
authors.lastname: Mechanic
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D003094:Collagen / Q000032:analysis*; D003488:Cyanogen Bromide / Q000378:metabolism; D003782:Dental Pulp / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D010434:Pepsin A / Q000378:metabolism; D012995:Solubility
chemical_list: D000596:Amino Acids; D003094:Collagen; D010434:Pepsin A; D003488:Cyanogen Bromide