Purification of human C-reactive protein by immunoaffinity chromatography using mouse monoclonal antibody.
Human C-reactive protein (CRP) was purified from sera with a high concentration of CRP by immunoaffinity chromatography using mouse monoclonal antibody (mAb) to CRP (No. 18) and ion exchange on DEAE-Sephacel. CRP bound to the immunoaffinity column was eluted by 0.5 M acetate buffer, pH 5.0 containing 1.5 M NaCl. Final recovery of CRP was 90%. No significant immunochemical differences were observed between CRP obtained by the immunoaffinity chromatography and CRP purified by the method of Hokama and Riley.
Nunomura
W
W
Tumour Laboratory, Tokyo, Japan.
Hatakeyama
M
M
Hirai
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002097:C-Reactive Protein / Q000276:immunology / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D005779:Immunodiffusion; D051379:Mice
D000911:Antibodies, Monoclonal; D002097:C-Reactive Protein