Natural anti-self reactivity which maps to MHC class I genes.
Responder spleen cells of [B10.A(5R) x B10.A(2R)]F1 hybrid mice cultured in vitro with irradiated parental spleen cells, generate a cytotoxic T cell response which maps to the H-2Dd and H-2(K-I)b genes of the B10.A(5R) parent. Specific reactivity of (5R x 2R) cells to B10.A(5R) parental (P) stimulators and not to F1 hybrid or B10.A(2R) stimulators was also demonstrated as an F1 anti-P proliferative response. Generation of a (5R x 2R) anti-5R response has also been noted during 2 degrees in vitro anti-viral cytotoxic responses where (5R x 2R) cells from either ectromelia or Sendai virus primed mice have been stimulated in vitro with infected 5R cells, but not when stimulated with infected 2R or (5R x 2R) cells. The generation of these responses is dependent on the use of parental stimulators for F1 hybrid cells during in vitro culture. All data is consistent with the hypothesis that (5R x 2R) F1 cells are not tolerant of 5R cells, and of the 4-fold higher level of Class I H-2Kb/Dd antigens which they express.
O'Neill
H C
HC
Developmental Haematology Group, John Curtin School of Medical Research, Australian National University, Canberra City.
D000818:Animals; D015551:Autoimmunity / Q000235:genetics*; D003602:Cytotoxicity, Immunologic; D005805:Genes, MHC Class I; D006183:H-2 Antigens / Q000235:genetics; D006824:Hybridization, Genetic; D007113:Immunity, Innate / Q000235:genetics; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D051381:Rats
D006183:H-2 Antigens