Isolation and characterization of cell clones persistently producing teardrop-shaped particles of human immunodeficiency virus type 1.
Several cell clones producing teardrop-shaped human immunodeficiency virus type 1 (HIV-1) particles were isolated from MT-4 cells that survived HIV-1 infection after extensive cell lysis. Most of the teardrop-shaped particles contained an electron-dense core structure, and the particles could replicate in MT-4 cells. No significant structural difference in the HIV-1 proteins was observed between these cell clones and the MOLT-4 cells producing intact HIV-1 particles, although their envelope structure is morphologically abnormal.
Morita
C
C
Department of Pathology, Osaka University, Japan.
Ikuta
K
K
Goto
T
T
Sano
K
K
Nakai
M
M
Hirai
K
K
Kato
S
S
D000118:Acetylglucosaminidase / Q000494:pharmacology; D002999:Clone Cells; D006026:Glycoside Hydrolases / Q000494:pharmacology; D015497:HIV-1 / Q000502:physiology / Q000648:ultrastructure*; D006801:Humans; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008854:Microscopy, Electron; D011233:Precipitin Tests; D014415:Tunicamycin / Q000494:pharmacology; D014771:Virion / Q000502:physiology / Q000648:ultrastructure
D014415:Tunicamycin; D006026:Glycoside Hydrolases; D000118:Acetylglucosaminidase; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase