Construction of broad-host-range plasmid vectors for easy visible selection and analysis of promoters.
We have constructed a series of broad-host-range plasmids which use "visual screens" to detect promoter activity. These plasmids contain the pMB1 and pRO1600 origins of replication and are capable of replicating in a wide range of gram-negative bacteria. The genes encoding beta-galactosidase and alkaline phosphatase from Escherichia coli and bacterial luciferase from Vibrio harveyi supply the promoterless indicator genes. The constructs were tested in E. coli and Pseudomonas aeruginosa.
Farinha
M A
MA
Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada.
Kropinski
A M
AM
D000469:Alkaline Phosphatase / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D007763:Lac Operon; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011550:Pseudomonas aeruginosa / Q000235:genetics
D000469:Alkaline Phosphatase