Botulinum ADP-ribosyltransferase activity as affected by detergents and phospholipids.
GTP-binding proteins with Mr values of 22,000 and 25,000 in bovine brain cytosol were ADP-ribosylated by an exoenzyme (termed C3) purified from Clostridium botulinum type C. The rate of C3-catalyzed ADP-ribosylation of the partially purified substrates was extremely low by itself, but was increased enormously when a protein factor(s) obtained from the cytosol was simultaneously added. The rate of the C3-catalyzed reaction was also stimulated by the addition of certain types of detergents or phospholipids even in the absence of the protein factors. The ADP-ribosylation appeared to be enhanced to an extent more than the additive effect of either the protein factors or the detergents (and phospholipids). Thus, ADP-ribosylation catalyzed by botulinum C3 enzyme was affected not only by cytoplasmic protein factors but also by detergents or phospholipids in manners different from each other.
Maehama
T
T
Department of Life Science, Faculty of Science, Tokyo Institute of Technology, Yokohama, Japan.
Ohoka
Y
Y
Ohtsuka
T
T
Takahashi
K
K
Nagata
K
K
Nozawa
Y
Y
Ueno
K
K
Ui
M
M
Katada
T
T
D036002:ADP Ribose Transferases / Q000378:metabolism*; D000818:Animals; D001905:Botulinum Toxins; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D003014:Clostridium botulinum / Q000201:enzymology*; D003902:Detergents / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D019204:GTP-Binding Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010743:Phospholipids / Q000494:pharmacology*; D013501:Surface-Active Agents / Q000494:pharmacology*
D003902:Detergents; D009419:Nerve Tissue Proteins; D010743:Phospholipids; D013501:Surface-Active Agents; D036002:ADP Ribose Transferases; C063007:exoenzyme C3, Clostridium botulinum; D001905:Botulinum Toxins; D019204:GTP-Binding Proteins