Influence of carbon dioxide on the surface characteristics and adherence potential of coagulase-negative staphylococci.
Coagulase-negative staphylococci obtained from patients with continuous ambulatory peritoneal dialysis-related peritonitis were cultured in nutrient broth in an atmosphere of air containing 5% carbon dioxide (reflecting the CO2 tension found in freshly used dialysate). Significant differences were observed between the surface chemistries of cells grown in the two atmospheres, as determined by X-ray photoelectron spectroscopy and changes in the cell wall protein profile. The growth atmosphere also influenced the adherence potential toward polystyrene and silicone in a proportion of strains examined. Thus, gaseous conditions can profoundly influence the nature of the staphylococcal surface, and this should be considered in any in vitro study of in vivo behavior.
Denyer
S P
SP
Department of Pharmaceutical Sciences, University of Nottingham, United Kingdom.
Davies
M C
MC
Evans
J A
JA
Finch
R G
RG
Smith
D G
DG
Wilcox
M H
MH
Williams
P
P
D001422:Bacterial Adhesion / Q000187:drug effects*; D002245:Carbon Dioxide / Q000494:pharmacology*; D006801:Humans; D010100:Oxygen / Q000494:pharmacology; D010531:Peritoneal Dialysis, Continuous Ambulatory; D011137:Polystyrenes; D012828:Silicones; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000254:growth & development; D013499:Surface Properties
D011137:Polystyrenes; D012828:Silicones; D002245:Carbon Dioxide; D010100:Oxygen