Method for obtaining bovine zygotes produced in vivo.
A superovulatory and surgical protocol was developed for recovery of bovine zygotes. Holstein cows and heifers were given follicle-stimulating hormone and cloprostenol to induce superovulation. Surgical cannulation and lavage of the uterine tube was performed 40 to 48 hours after the start of standing estrus. In general, cows had more corpora hemorrhagica than did heifers, but a higher percentage (P less than 0.05) of ova recovered from cows were infertile. Several heifers were subjected to the procedure twice, and embryo recovery rates were equivalent both times.
Ellington
J E
JE
Department of Animal Science, New York State College of Agriculture and Life Sciences, Cornell University, Ithaca 14853-4801.
Farrell
P B
PB
Simkin
M E
ME
Foote
R H
RH
D000367:Age Factors; D000818:Animals; D002404:Catheterization / Q000379:methods / Q000662:veterinary*; D002417:Cattle; D003008:Cloprostenol / Q000494:pharmacology; D004624:Embryo Transfer / Q000379:methods / Q000662:veterinary; D004971:Estrus / Q000502:physiology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000502:physiology; D010062:Ovulation Induction / Q000662:veterinary*; D011247:Pregnancy; D013480:Superovulation; D007507:Therapeutic Irrigation / Q000662:veterinary; D015053:Zygote
D003008:Cloprostenol; D005640:Follicle Stimulating Hormone