Effect of long-term administration of insulin and glibenclamide on pancreatic A and B cell function.
To investigate the effects of insulin and sulfonylurea drug administration on islet A and B-cell functions, insulin and glucagon response was studied in normal rats treated with insulin (4 U/day subcutaneously) or glibenclamide (0.03 mg/kg intraperitoneally) for 8 weeks. In intravenous glucose tolerance test (0.5 g/kg), lower insulin response and impaired glucose tolerance were observed in insulin-treated rats and higher insulin response and impaired glucose tolerance were observed in glibenclamide-treated rats. In perfused pancreas, insulin response to glucose and arginine was significantly lower in insulin-treated rats and that was significantly higher in glibenclamide-treated rats. Glucagon response was not altered by insulin or glibenclamide treatment. These results suggest that hyperinsulinisation and glibenclamide administration strongly influence pancreatic B-cell function but not influence pancreatic A-cell function.
Ikeda
T
T
First Department of Internal Medicine, Tottori University School of Medicine, Japan.
Fujiyama
K
K
Hoshino
T
T
Takeuchi
T
T
Mashiba
H
H
Tominaga
M
M
D000818:Animals; D001120:Arginine / Q000494:pharmacology; D001786:Blood Glucose / Q000378:metabolism; D005934:Glucagon / Q000378:metabolism; D005951:Glucose Tolerance Test; D005905:Glyburide / Q000008:administration & dosage / Q000494:pharmacology*; D006946:Hyperinsulinism / Q000378:metabolism; D007328:Insulin / Q000008:administration & dosage / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D001786:Blood Glucose; D007328:Insulin; D005934:Glucagon; D001120:Arginine; D005905:Glyburide