Identification of cis-acting sequences required for translational autoregulation of the ermC methylase.
ermC methylase gene expression has been shown to be limited by translational autorepression, presumably due to methylase binding to ermC mRNA. It was found that this repression occurs in trans, yielding a 50% reduction in translation of an ermC-lacZ fusion mRNA. We investigated the ermC mRNA sequences required for translational repression in vivo. A series of deletions identified sequences in the 5' regulatory region that were required for translational repression. These included sequences of the 5' stem-loop structure that were not required for induction, as well as some that were required. The implications of these results for regulation are discussed.
Breidt
F
F
Department of Microbiology, Public Health Research Institute, New York, New York 10016.
Dubnau
D
D
D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006706:Homeostasis; D008780:Methyltransferases / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D015345:Oligonucleotide Probes; D010957:Plasmids; D014176:Protein Biosynthesis; D012335:RNA, Ribosomal / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis
D004269:DNA, Bacterial; D015345:Oligonucleotide Probes; D012335:RNA, Ribosomal; D011993:Recombinant Fusion Proteins; D008780:Methyltransferases