title: The psbA-gene from a red alga resembles those from cyanobacteria and cyanelles.
abstract: Plastid DNA (ptDNA) from the unicellular red alga Cyanidium caldarium was isolated. A 5.8 kb Eco RI, fragment containing the entire psbA-gene was cloned and the nucleotide sequence of the psbA-gene determined. At the carboxyl terminus the encoded protein (D1) contains the seven amino acid-insertion which was found to be typical of the cyanobacteria and the cyanelles of Cyanophora paradoxa. However, the overall sequence homology does not support a direct relationship between the plastids of Cyanidium, cyanelles and the cyanobacteria. As in other photosynthetic organisms the psbA-gene is transcribed as a monocistronic mRNA. The ribosomal RNA operon was located 4 kb upstream of the psbA-gene.
authors.affiliation: Institut für Pflanzenphysiologie, Justus-Liebig-Universität Giessen, Federal Republic of Germany.
authors.forename: U
authors.initials: U
authors.lastname: Maid
authors.forename: K
authors.initials: K
authors.lastname: Valentin
authors.forename: K
authors.initials: K
authors.lastname: Zetsche
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002734:Chlorophyll / Q000235:genetics*; D002736:Chloroplasts; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005796:Genes; D045342:Light-Harvesting Protein Complexes; D008969:Molecular Sequence Data; D009876:Operon; D045322:Photosynthetic Reaction Center Complex Proteins; D010940:Plant Proteins / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics; D015183:Restriction Mapping; D000461:Rhodophyta / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D010940:Plant Proteins; D012335:RNA, Ribosomal; D002734:Chlorophyll; D004247:DNA