title: Changes in the concentrations of cerebral proteins following occlusion of the middle cerebral artery in rats.
abstract: Using an immunoblotting technique, we investigated changes in the concentrations of microtubule-associated protein 2, 200-kDa neurofilament, tubulin, myelin-associated glycoprotein, and 2':3'-cyclic nucleotide 3'-phosphodiesterase in the brains of 40 rats following occlusion of the left middle cerebral artery or sham operation. Compared with those 4 hours after surgery, concentrations of all proteins decreased significantly in the left hemisphere 3 days after surgery (p less than 0.01). Microtubule-associated protein 2 was the most susceptible to ischemia, and its mean +/- SEM concentration decreased to 23 +/- 9.4% of that in concurrent sham-operated controls. Degradation products of microtubule-associated protein 2 and myelin-associated glycoprotein were detected on the blots. Furthermore, in the contralateral hemisphere (where calpain might be activated), concentrations of these two proteins decreased to 57 +/- 12.0% and 83 +/- 4.3% of those in concurrent sham-operated controls, respectively, 3 days after surgery. Changes in the concentrations of cerebral proteins in the contralateral hemisphere are important for understanding clinical symptoms not attributable solely to the ipsilateral lesion following a focal cerebral stroke.
authors.affiliation: Department of Neurology, Niigata University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Inuzuka
authors.forename: A
authors.initials: A
authors.lastname: Tamura
authors.forename: S
authors.initials: S
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Kirino
authors.forename: K
authors.initials: K
authors.lastname: Yanagisawa
authors.forename: I
authors.initials: I
authors.lastname: Toyoshima
authors.forename: T
authors.initials: T
authors.lastname: Miyatake
mesh_terms: D000818:Animals; D001157:Arterial Occlusive Diseases / Q000378:metabolism; D001923:Brain Chemistry; D002545:Brain Ischemia / Q000378:metabolism*; D002539:Cerebral Arterial Diseases / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis*; D007381:Intermediate Filament Proteins / Q000032:analysis*; D008297:Male; D008869:Microtubule-Associated Proteins / Q000032:analysis*; D008970:Molecular Weight; D009185:Myelin Proteins / Q000032:analysis*; D016900:Neurofilament Proteins; D051381:Rats; D014404:Tubulin / Q000032:analysis
chemical_list: D006023:Glycoproteins; D007381:Intermediate Filament Proteins; D008869:Microtubule-Associated Proteins; D009185:Myelin Proteins; D016900:Neurofilament Proteins; D014404:Tubulin