Coordinate expression of src family protooncogenes in T cell activation and its modulation by cyclosporine.
Activation of T lymphocytes induces transcription of several important genes which encode lymphokines and lymphokine receptors as well as "proliferation complementary" protooncogenes like c-myc or c-fos. Recently, the expression of lck gene, one of the src family gene, has also been shown to be modulated during T cell activation. We, therefore, assessed the question of whether other src family genes are expressed during the activation of T cells and of whether cyclosporine, a potent immunosuppressive drug, affects expression of these genes. We examined the expression of four different src family genes (lck, c-src, fyn, and c-fgr) in addition to the expression of IL-2, c-fos, c-myc, and actin genes in murine T cells which were activated with PMA plus ionomycin or PMA plus anti-CD3 mAb. We found that T cell activation was associated with the up-regulation of these src family genes and that the expression of these genes was specifically blocked in the presence of cyclosporine indicating that these activation-related genes were coordinately regulated.
Furue
M
M
Dermatology Branch, National Cancer Institute, Bethesda, MD 20892.
Katz
S I
SI
Kawakami
Y
Y
Kawakami
T
T
D015152:Blotting, Northern; D015153:Blotting, Western; D003524:Cyclosporins / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics; D015759:Ionomycin / Q000494:pharmacology; D008213:Lymphocyte Activation; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D005810:Multigene Family; D015688:Oncogene Protein pp60(v-src) / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics; D016760:Proto-Oncogene Proteins c-fos; D051076:Proto-Oncogene Proteins c-fyn; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000235:genetics; D013601:T-Lymphocytes / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D019061:src-Family Kinases
D003524:Cyclosporins; D007376:Interleukin-2; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D015759:Ionomycin; C494957:FYN protein, human; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D015688:Oncogene Protein pp60(v-src); D051076:Proto-Oncogene Proteins c-fyn; C053102:proto-oncogene proteins c-fgr; D019061:src-Family Kinases; D013755:Tetradecanoylphorbol Acetate