title: Internal transcribed spacer 1 of the yeast precursor ribosomal RNA. Higher order structure and common structural motifs.
abstract: The higher order structure of the first internal transcribed spacer between the 18S and the 5.8S rRNA sequences in the Saccharomyces cerevisiae precursor ribosomal RNA has been investigated. Sites of potential base pairing in the RNA region have been determined by using a combination of enzymatic and chemical structure sensitive probes. Data generated have been used to evaluate secondary structure models predicted by minimum free energy calculations. Several alternative suboptimal structures were also evaluated. The derived model contains several stable hairpins. Theoretical secondary structural models for the corresponding RNA region from S. carlsbergensis, S. pombe, N. crassa, X. laevis, and mung bean have also been derived from identical calculations and assumptions. Certain structural motifs appear to be conserved despite extensive divergence in the base sequence. The yeast model should be a useful prototype for investigation of structure and function of precursor ribosomal RNA molecules.
authors.affiliation: Department of Biochemistry, University of Texas Health Science Center, San Antonio 78284-7760.
authors.forename: L C
authors.initials: LC
authors.lastname: Yeh
authors.forename: R
authors.initials: R
authors.lastname: Thweatt
authors.forename: J C
authors.initials: JC
authors.lastname: Lee
mesh_terms: D000477:Alkylating Agents / Q000378:metabolism; D001345:Autoradiography; D001483:Base Sequence; D003028:CME-Carbodiimide / Q000031:analogs & derivatives*; D002234:Carbodiimides / Q000378:metabolism; D003198:Computer Simulation; D004722:Endoribonucleases / Q000378:metabolism; D008956:Models, Chemical; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides; D012322:RNA Precursors / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism; D006163:Ribonuclease T1 / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013463:Sulfuric Acid Esters / Q000378:metabolism; D014158:Transcription, Genetic / Q000502:physiology
chemical_list: D000477:Alkylating Agents; D002234:Carbodiimides; D009838:Oligodeoxyribonucleotides; D012322:RNA Precursors; D012335:RNA, Ribosomal; D013463:Sulfuric Acid Esters; C060331:1-cyclohexyl-3-(2-(4-morpholinyl)ethyl)carbodiimide; D003028:CME-Carbodiimide; D004722:Endoribonucleases; C032825:ribonuclease V(1); D006163:Ribonuclease T1; C007482:dimethyl sulfate