[Microspectrofluorometric analysis of the effect of centrophenoxine on lipofuscin granules of hybridoma (Retrovirus-transformed) cells].
The increase of the own luminescence lipofuscin granules (LG) in the course of single and repeated ultraviolet (UV) excitations (365 nm) in hybridoma (retrovirus transformed) cells cultured with or without 5 x 10(-4) M centrophenoxine (CP) was studied by microspectrofluorometric method. It was shown that CP influences only the rate of photochemical changes of chromophores in LG. Kinetic patterns of the own luminescence intensity of LG at the wavelength of 540 nm during excitation by UV permit one to suggest the occurrence of the cyclic chromophore changes.
Tatariunas
A B
AB
D000818:Animals; D002472:Cell Transformation, Viral; D003594:Cytoplasmic Granules / Q000032:analysis / Q000187:drug effects*; D006016:Glycolates / Q000494:pharmacology*; D006825:Hybridomas / Q000648:ultrastructure*; D066298:In Vitro Techniques; D008062:Lipofuscin / Q000032:analysis*; D008163:Luminescent Measurements; D002504:Meclofenoxate / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000473:pathology; D010860:Pigments, Biological / Q000032:analysis*; D012190:Retroviridae; D013050:Spectrometry, Fluorescence / Q000379:methods; D013154:Spleen / Q000166:cytology
D006016:Glycolates; D008062:Lipofuscin; D010860:Pigments, Biological; D002504:Meclofenoxate
Mikrospektrofliuorimetricheskiĭ analiz deĭstviia tsentro- fenoksina na lipofustsinovye granuly gibridomnykh (retrovirus- transformirovannykh) kletok.