Determination of alpha-tocopherol in plasma, platelets and erythrocytes of type I and type II diabetic patients by high-performance liquid chromatography.
Vitamin E (alpha-tocopherol) concentrations of plasma, platelets and erythrocytes were determined by HPLC in insulin-dependent (type I) and age-matched non-insulin-dependent (type II) diabetic patients and in two control groups. Plasma alpha-tocopherol levels were significantly increased in diabetic patients compared to control groups. Platelet and erythrocyte alpha-tocopherol levels were not significantly different in type I and type II diabetics as compared to their respective control groups, but differed from one another. Plasma vitamin E concentrations showed a significant correlation with plasma cholesterol and apoprotein B concentrations in different groups. The alpha-tocopherol/cholesterol and alpha-tocopherol/apoprotein B ratios in plasma were higher in diabetic patients, as were triglyceride contents. Platelet vitamin E levels were not significantly correlated with plasma concentrations. These findings suggest that vitamin E activity is altered in diabetic patients but that no diet supplementation seems necessary.
Caye-Vaugien
C
C
Laboratoire de Biochimie, Faculté des Sciences et des Techniques, Nantes, France.
Krempf
M
M
Lamarche
P
P
Charbonnel
B
B
Pieri
J
J
D000328:Adult; D000368:Aged; D001055:Apolipoproteins B / Q000097:blood; D001792:Blood Platelets / Q000378:metabolism*; D002784:Cholesterol / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D009752:Nutritional Status; D014280:Triglycerides / Q000097:blood; D014810:Vitamin E / Q000097:blood*
D001055:Apolipoproteins B; D014280:Triglycerides; D014810:Vitamin E; D002784:Cholesterol