Is the inhibitory effect of progesterone on endometrial prostaglandin F2 alpha production due to an inhibition of protein synthesis?
Progesterone and a high concentration of oestradiol (i) reduced the outputs of prostaglandin (PG) F2 alpha and, to a lesser extent, PGE2 from Day-7 and Day-15 guinea-pig endometrium in culture, but had little or no effect on the output of 6-keto-PGF1 alpha, (ii) prevented the increase in PGH synthase concentrations which normally occur in Day-7 and Day-15 guinea-pig endometrium during culture, and (iii) reduced the synthesis of secreted proteins by Day-15 guinea-pig endometrium in culture. These findings suggest that the inhibitory effect of progesterone and of high concentrations of oestradiol on endometrium PGF2 alpha synthesis is due to an inhibition of the syntheses of proteins involved in PGF2 alpha production.
Riley
S C
SC
Department of Pharmacology, University of Edinburgh Medical School, Scotland, UK.
Poyser
N L
NL
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D000818:Animals; D016861:Cyclooxygenase Inhibitors; D015237:Dinoprost / Q000096:biosynthesis*; D015232:Dinoprostone / Q000096:biosynthesis*; D004717:Endometrium / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D011374:Progesterone / Q000494:pharmacology*; D014176:Protein Biosynthesis
D016861:Cyclooxygenase Inhibitors; D011374:Progesterone; D004958:Estradiol; D015121:6-Ketoprostaglandin F1 alpha; D015237:Dinoprost; D015232:Dinoprostone; D007213:Indomethacin