Fatty acid binding protein (FABP) modulates prostaglandin E binding to rat epididymal adipocyte membrane similarly to albumin.
Albumin enhances prostaglandin E2 (PGE2) binding to isolated epididymal adipocyte membrane and also binds PGE2 with low affinity. On the other hand, S-100, ovalbumin and albumin-stearate failed to bind PGE2, as shown by ultrafiltration, and also failed to enhance PGE2 binding to the isolated adipocyte membranes. These results suggested that albumin enhances PGE2 binding possibly by serving as a carrier for the prostaglandin molecules. 3 mM warfarin or 1 mM phenylbutazone inhibited PGE2 binding to albumin by 70% and 95%, respectively, but both drugs failed to affect the enhancement of PGE2 binding to the isolated adipocyte membrane in the presence of albumin. These results exclude the possibility that PGE2 bound to albumin is more accessible to the prostaglandin receptor than free PGE2 in solution. Finally it is shown that fatty acid binding protein (FABP), a cytosolic protein which binds specifically PGE1 but not PGE2, enhances PGE1 and PGE2 binding to isolated adipocyte membranes similarly to albumin. The physiological implications of these findings are discussed.
Cohen-Luria
R
R
Department of Clinical Pharmacology, Faculty of Health Sciences, Ben-Gurion University, Beer Sheva, Israel.
Danon
A
A
Rimon
G
G
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000494:pharmacology*; D002462:Cell Membrane / Q000378:metabolism; D003975:Diazepam / Q000494:pharmacology; D015232:Dinoprostone / Q000378:metabolism*; D004822:Epididymis / Q000378:metabolism; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D010653:Phenylbutazone / Q000494:pharmacology; D011485:Protein Binding; D051381:Rats; D012709:Serum Albumin / Q000378:metabolism*; D014859:Warfarin / Q000494:pharmacology
D002352:Carrier Proteins; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D012709:Serum Albumin; D014859:Warfarin; D019204:GTP-Binding Proteins; D010653:Phenylbutazone; D015232:Dinoprostone; D003975:Diazepam