New trends in photobiology. The interaction of UVA radiation with cultured cells.
Recent work concerning the interaction of UVA radiation (320-380 nm) with cultured cells is reviewed with particular emphasis on the involvement of cellular oxidative stress in the biological effects of this radiation on eucaryotic cells. Possible chromophores are considered and their role in generation of various oxidant species including hydrogen peroxide, superoxide anion, singlet oxygen and hydroxyl radical. DNA and membranes are discussed as possible targets for the lethal action of long wavelength radiation. Four mechanisms of cellular defence are proposed: (1) DNA repair; (2) antioxidant enzymes; (3) endogenous free radical quenchers; (4) inducible protection.
Tyrrell
R M
RM
ISREC, Epalinges, Switzerland.
Keyse
S M
SM
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002478:Cells, Cultured; D004247:DNA / Q000528:radiation effects; D004260:DNA Repair / Q000528:radiation effects; D005609:Free Radicals; D006419:Heme Oxygenase (Decyclizing) / Q000528:radiation effects; D006801:Humans; D008566:Membranes / Q000528:radiation effects; D010100:Oxygen / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects*
D000975:Antioxidants; D005609:Free Radicals; D004247:DNA; D006419:Heme Oxygenase (Decyclizing); D010100:Oxygen