The in vitro biotransformation of 2-aminofluorene in the visceral mass of the Pacific oyster, Crassostrea gigas.
1. The in vitro biotransformation of 2-aminofluorene (2-AF) was examined in visceral mass microsomes from the Pacific oyster Crassostrea gigas. 2. The major metabolite was N-formyl-2-aminofluorene with smaller quantities of N-hydroxy-2-aminofluorene and nitroso-2-aminofluorene or 2-nitrofluorene. 3. The mechanism of N-oxidation was explored using various inhibitors, stimulators, cofactors, and pH modifications within incubations. 4. N-Oxidation may occur via a one electron mechanism which involves a transition metal and/or lipid peroxidation. 5. Flavin-containing monooxygenase and possibly prostaglandin synthase also seem to play a role in N-oxidation.
Schlenk
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Duke University Marine Laboratory, Beaufort, NC 28516.
Buhler
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D000818:Animals; D001711:Biotransformation; D002374:Catalase / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005449:Fluorenes / Q000378:metabolism / Q000493:pharmacokinetics*; D005978:Glutathione / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D009249:NADP / Q000494:pharmacology; D010125:Ostreidae / Q000378:metabolism*; D010084:Oxidation-Reduction; D013482:Superoxide Dismutase / Q000494:pharmacology
D005449:Fluorenes; C012177:2-aminofluorene; D009249:NADP; D004492:Edetic Acid; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione