Characterization of the v-myb DNA binding domain.
The transforming protein encoded by the v-myb oncogene is a sequence-specific DNA-binding protein that is thought to be involved in the regulation of gene expression. The N-terminal region of the v-myb protein is composed of two highly conserved tandem repeat sequences of unknown function. It has been speculated that the N-terminal v-myb repeats might be crucial for DNA-binding, since N-terminal deletions destroy the DNA-binding activity of the v-myb protein. Here, we have studied the v-myb DNA-binding domain in more detail. Our results show that the N-terminal region of the v-myb protein is sufficient for specific DNA-binding. Dissection of this region suggests that both repeats are required for DNA-binding, but that both repeats play different roles in v-myb protein DNA interaction. We also show that the myb repeats of a drosophila melanogaster homolog of c-myb function as sequence-specific DNA-binding domain. Our results support the view that specific sequence-recognition, mediated by the conserved myb repeats, is a general feature of myb-related proteins.
Oehler
T
T
Zentrum für Molekulare Biologie, Universität Heidelberg, FRG.
Arnold
H
H
Biedenkapp
H
H
Klempnauer
K H
KH
D000818:Animals; D009189:Avian Myeloblastosis Virus / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D002872:Chromosome Deletion; D004268:DNA-Binding Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D020626:Oncogene Proteins v-myb; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics* / Q000378:metabolism
D004268:DNA-Binding Proteins; D015345:Oligonucleotide Probes; D020626:Oncogene Proteins v-myb; D011994:Recombinant Proteins; D015682:Retroviridae Proteins, Oncogenic; D011505:Protein-Tyrosine Kinases