Dengue virus serotypic identification using suckling mouse and western blot technique.
The suckling mouse which is used in the classical method to detect and propagate dengue viruses was evaluated in conjunction with the western blot and immunoenzymatic methods to detect the infecting strains of dengue viruses. After intracerebral inoculation of patients' sera into the suckling mice for 7 days, the mice were examined for the presence of dengue proteins, even though the mice did not have the neurological symptoms which usually serve as an indicator for the presence of dengue infection in the mouse brain. With a blind study of a set of 12 specimens, the suckling mice could detect the virus with the same frequency as the mosquito system but in shorter time of incubation period. The whole process to identify the type of infection takes 9 days. Another important finding is the demonstration of the virion antigen in the liver. The quantity and quality of viral proteins in liver are comparable to those in the brain suggesting that the virus may replicate in the liver as well as in the brain.
Churdboonchart
V
V
Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Khemarunmanus
M
M
Yoksan
S
S
Bhamarapravati
N
N
D000818:Animals; D000833:Animals, Suckling; D015153:Blotting, Western / Q000379:methods / Q000592:standards*; D003715:Dengue / Q000145:classification / Q000175:diagnosis / Q000453:epidemiology*; D005069:Evaluation Studies as Topic; D007124:Immunoenzyme Techniques / Q000592:standards*; D051379:Mice; D012680:Sensitivity and Specificity; D012703:Serotyping