title: [Affinity chromatographic purification of streptokinase with monoclonal antibodies].
abstract: Monoclonal antibodies against streptokinase as ligands were coupled to different matrices. Both CNBr-activated Sepharose and macroporous bead cellulose activated by carbonochloridate revealed as suitable for purification of streptokinase by affinity chromatography. Immunoadsorbents with a higher concentration of the coupled ligand were more effective than those with a lower. For streptokinase optimal conditions of binding and elution without negative influence on structure and activity were ascertained. A buffer with slight alkaline pH was successful for desorption. Using this method it was possible to obtain pure streptokinase from several streptokinase containing media.
authors.affiliation: Bereich Tierphysiologie und Immunobiologie, Karl-Marx-Universität Leipzig.
authors.forename: E M
authors.initials: EM
authors.lastname: Andreas
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D007158:Immunologic Techniques; D008970:Molecular Weight; D013291:Streptococcus / Q000201:enzymology; D013300:Streptokinase / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D013300:Streptokinase
vernacular_title: Affinitätschromatographische Reinigung von Streptokinase mittels monoklonaler Antikörper.