title: Histochemical and immunohistochemical evidence for hepatic zone 3 distribution of alcohol dehydrogenase in rats.
abstract: The distribution of alcohol dehydrogenase in the hepatic acinus was examined by both histochemical and immunohistochemical approaches. The immunohistochemical method using anti-alcohol dehydrogenase antibody indicated zone 3 predominance of this enzyme in the hepatic acinus, whereas a conventional histochemical method showed slight zone 1 predominance. However, when the histochemical technique was improved by using 2% glutaraldehyde instead of formalin for fixation and by adding phenazine methosulfate (0.33 mmol/L) to the staining incubation mixture, this method also supported zone 3 predominance of alcohol dehydrogenase. Evidence for zone 3 distribution of alcohol dehydrogenase may be of value in elucidating the mechanism of zone 3 liver damage by alcohol.
authors.affiliation: Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kato
authors.forename: H
authors.initials: H
authors.lastname: Ishii
authors.forename: S
authors.initials: S
authors.lastname: Aiso
authors.forename: S
authors.initials: S
authors.lastname: Yamashita
authors.forename: D
authors.initials: D
authors.lastname: Ito
authors.forename: M
authors.initials: M
authors.lastname: Tsuchiya
mesh_terms: D000426:Alcohol Dehydrogenase / Q000032:analysis*; D000818:Animals; D002478:Cells, Cultured; D005557:Formaldehyde; D005976:Glutaral; D006651:Histocytochemistry; D006652:Histological Techniques; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008297:Male; D008773:Methylphenazonium Methosulfate; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007202:Indicators and Reagents; D005557:Formaldehyde; D008773:Methylphenazonium Methosulfate; D000426:Alcohol Dehydrogenase; D005976:Glutaral