Complete sequence of human vinculin and assignment of the gene to chromosome 10.
We have determined the complete sequence of human vinculin, a cytoskeletal protein associated with cell-cell and cell-matrix junctions. Comparison of human and chicken embryo vinculin sequences shows that both proteins contain 1066 amino acids and exhibit a high level of sequence identity (greater than 95%). The region of greatest divergence falls within three 112-amino acid repeats spanning residues 259-589. Interestingly, nematode vinculin lacks one of these central repeats. The regions of human vinculin that are N- and C-terminal to the repeats show 54% and 61% sequence identity, respectively, to nematode vinculin. Southern blots of human genomic DNA hybridized with short vinculin cDNA fragments indicate that there is a single vinculin gene. By using a panel of human-rodent somatic cell hybrids, the human vinculin gene was mapped to chromosome 10q11.2-qter.
Weller
P A
PA
Department of Biochemistry, University of Leicester, United Kingdom.
Ogryzko
E P
EP
Corben
E B
EB
Zhidkova
N I
NI
Patel
B
B
Price
G J
GJ
Spurr
N K
NK
Koteliansky
V E
VE
Critchley
D R
DR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D002879:Chromosomes, Human, Pair 10; D003598:Cytoskeletal Proteins / Q000235:genetics*; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D016596:Vinculin
D003598:Cytoskeletal Proteins; D016596:Vinculin