Gamma interferon-induced specific binding of tetanus toxin on the GG2EE macrophage cell line.
We have recently demonstrated that tetanus toxin (TT) selectively inhibited gamma interferon-(IFN-gamma)-induced, but not basal, lysozyme activity in the GG2EE macrophage cell line. By an indirect immunofluorescence technique, we show that tetanus toxin, as well as the BIIb tetanus toxin-derived fragment, selectively binds to IFN-gamma-treated cells but fails to bind to control cells. Moreover, BIIb fragment and TT share the same binding sites, as demonstrated by competition/displacement experiments. These data indicate that IFN-gamma pretreatment results in the acquisition of tetanus toxin binding sites on GG2EE cells by a mechanism(s) yet to be identified.
Blasi
E
E
Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
Pitzurra
L
L
Burhan Fuad
A M
AM
Marconi
P
P
Bistoni
F
F
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D001667:Binding, Competitive; D002460:Cell Line; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D007136:Immunoglobulins / Q000276:immunology; D007371:Interferon-gamma / Q000494:pharmacology*; D008264:Macrophages / Q000378:metabolism*; D013744:Tetanus Toxin / Q000378:metabolism*; D013861:Thiocyanates
D000888:Antibodies, Anti-Idiotypic; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007136:Immunoglobulins; D013744:Tetanus Toxin; D013861:Thiocyanates; D007371:Interferon-gamma; D016650:Fluorescein-5-isothiocyanate