[Inhibition of translation of immunoglobulin mRNA in vitro using an alkylating oligonucleotide derivative].
Effect of complementary oligonucleotides and their reactive derivatives on translation of mouse immunoglobulin G kappa light chain was investigated. It was found that oligonucleotide pTGCTCTGGTTT and shorter oligonucleotides complementary to the coding sequence of the mRNA (nucleotides 205-215) do not arrest translation of the mRNA in the rabbit reticulocyte cell-free translation system. Preincubation of the mRNA with the alkylating 4-(N-2-chloroethyl-N-methylamino)benzyl-5'-phosphamide derivative of the oligonucleotide completely suppresses the synthesis of the protein thus demonstrating higher efficiency of the reactive oligonucleotide derivatives as inhibitors of the mRNA function.
Vlasov
V V
VV
Godovikov
A A
AA
Zarytova
V F
VF
Ivanova
E M
EM
Nomokonova
N Iu
NIu
D000477:Alkylating Agents / Q000494:pharmacology*; D000818:Animals; D002474:Cell-Free System; D004591:Electrophoresis, Polyacrylamide Gel; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D009841:Oligonucleotides / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects*; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism
D000477:Alkylating Agents; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D009841:Oligonucleotides; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger
Podavlenie transliatsii immunoglobulinovoĭ mRNK in vitro s pomoshch'iu alkiliruiushchego proizvodnogo oligonukleotida.