Isolation of a Trypanosoma cruzi antigen by affinity chromatography with a monoclonal antibody. Preliminary evaluation of its possible applications in serological tests.
By affinity chromatography with a monoclonal antibody (163B6), obtained in our laboratory, we have isolated a T. cruzi antigen which could be useful for differential diagnosis of Chagas' disease from leishmaniasis. This antigen, a 52-kD protein, reacted with all sera from Chagas' disease patients tested but not with sera from patients with leishmania, in ELISA. The 52-kD antigen is widely distributed in the Trypanosoma genus since the 163B6 monoclonal antibody reacts with T. rangeli and 8 strains and a clone of T. cruzi epimastigotes.
Carbonetto
C H
CH
Institute of Humoral Immunity Studies (IDEHU) CONICET/UBA, Department of Immunology, Faculty of Pharmacology and Biochemistry, Argentina.
Malchiodi
E L
EL
Chiaramonte
M
M
Durante de Isola
E
E
Fossati
C A
CA
Margni
R A
RA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology / Q000302:isolation & purification*; D014355:Chagas Disease / Q000097:blood / Q000175:diagnosis*; D002846:Chromatography, Affinity / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007132:Immunoglobulin Isotypes; D007896:Leishmaniasis / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D014349:Trypanosoma cruzi / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D007132:Immunoglobulin Isotypes