Investigation of the SOS response of Escherichia coli after gamma-irradiation by means of the SOS chromotest.
The kinetics of SOS system induction in Escherichia coli PQ37 cells by gamma-irradiation has been studied by the SOS chromotest technique. It was shown that the synthesis of constitutive alkaline phosphatase is not immediately stopped in cells that suffered lethal damages from gamma-irradiation. The production of DNA damages inducing the SOS system was 0.021/Gy per genome. The SOS system was switched off approximately 200 min after gamma-irradiation. A correction is proposed to the calculation of the SOS system induction factor.
Kozubek
S
S
Joint Institute for Nuclear Research, Dubna, U.S.S.R.
Ogievetskaya
M M
MM
Krasavin
E A
EA
Drasil
V
V
Soska
J
J
D000469:Alkaline Phosphatase / Q000096:biosynthesis; D004249:DNA Damage; D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects; D005720:Gamma Rays; D013014:SOS Response, Genetics; D013997:Time Factors; D001616:beta-Galactosidase / Q000378:metabolism
D000469:Alkaline Phosphatase; D001616:beta-Galactosidase