title: Production of an abnormal mu chain with a shortened VHIV subgroup variable region in a Burkitt's lymphoma cell line.
abstract: The Burkitt's lymphoma cell line Ly66 produces a short mu chain which lacks 4 kDa in apparent molecular mass. Study of the corresponding messenger RNA showed it to be 0.3 kb shorter than normal mu transcripts. The cDNA sequence of the mu transcripts began by a short VH region consisting of the first one-third of a VHIV subgroup gene segment. It was followed by a normal mu constant region. This VH region coded for 38 amino acids, thus differing from two truncated VHIV regions previously reported in other Burkitt's lymphoma cell lines, which were interrupted at codon +26 by an alternate splicing event. In addition, the Ly66 variable sequence bore several point mutations and contained two potential N-glycosylation sites.
authors.affiliation: CNRS URA 1172, Laboratoire d'Immunologie Moléculaire, Faculté des Sciences and Laboratoire d'Immunologie et d'Immunopathologie, Hôpital Universitaire, Poitiers, France.
authors.forename: M
authors.initials: M
authors.lastname: Cogné
authors.forename: S
authors.initials: S
authors.lastname: Mounir
authors.forename: T
authors.initials: T
authors.lastname: Mahdi
authors.forename: J L
authors.initials: JL
authors.lastname: Preud'homme
authors.forename: F
authors.initials: F
authors.lastname: Nau
authors.forename: P
authors.initials: P
authors.lastname: Guglielmi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002051:Burkitt Lymphoma / Q000235:genetics*; D003001:Cloning, Molecular; D006031:Glycosylation; D006801:Humans; D007127:Immunoglobulin Constant Regions / Q000235:genetics; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012326:RNA Splicing; D014407:Tumor Cells, Cultured
chemical_list: D007127:Immunoglobulin Constant Regions; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region