A monoclonal antibody recognizing selectively rat granulocytes.
In the present study, a murine monoclonal antibody, RK-4 (IgG1) specific for rat granulocytes and their precursors in blood and bone marrow is described. Results obtained by immunocytological and flowcytometry analysis showed this mAb to recognize selectively a surface antigen on neutrophils, eosinophils and basophils. Analysis of cryosections of various lymphatic and non-lymphatic organs demonstrates that this mouse antibody does not cross-react with any other structures. By flow cytometric analysis we found peripheral blood leukocytes to consist of about 15-20% granulocytes. RK-4 is therefore a valuable tool for studying differentiation and function of granulocytes under various conditions.
Steinhausen
F
F
Department of Immunology, University of Göttingen, Germany.
Xu
H
H
Kuhn
R
R
Gröne
H J
HJ
Peters
J H
JH
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001491:Basophils / Q000166:cytology / Q000276:immunology; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D004804:Eosinophils / Q000166:cytology / Q000276:immunology; D005434:Flow Cytometry; D006098:Granulocytes / Q000166:cytology / Q000276:immunology*; D007074:Immunoglobulin G; D009504:Neutrophils / Q000166:cytology / Q000276:immunology; D051381:Rats
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G