Study of X chromosome abnormality in XX males using bivariate flow karyotype analysis and flow sorted dot blots.
We have used bivariate flow karyotype analysis to quantify aberrant X chromosome size in 11 XX males. With one exception, the patients could be grouped into those with an X homologue difference greater than normal (Group A, n = 3) and into those whose X homologue difference could not be distinguished from female controls (Group B, n = 7). The range of sizes of the aberrant X chromosome in Y-sequence positive patients agrees with the variable nature of the X-Y interchange in these individuals as determined by the use of Y-specific DNA probes and Southern blotting analysis. In one patient it was possible to sort separately the normal and the X-Y interchanged homologues for dot blot analysis. The presence of Y sequences and an increased dose of the zinc finger gene, ZFY, were detected in the X-Y interchanged homologue. In preliminary studies of 5 male and 6 female controls, it was noted that a consistent difference between the two X homologues in females was found which could not be totally explained by errors of the fitting procedure. We suggest that this difference could be due to X inactivation and that the two X homologues in females might be distinguishable.
Carter
N P
NP
Department of Pathology, University of Cambridge, England.
Ferguson-Smith
M E
ME
Affara
N A
NA
Briggs
H
H
Ferguson-Smith
M A
MA
D001345:Autoradiography; D002458:Cell Fractionation; D014128:Chromomycin A3; D015342:DNA Probes; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D015151:Immunoblotting; D007621:Karyotyping; D008297:Male; D009693:Nucleic Acid Hybridization; D012729:Sex Chromosome Aberrations / Q000235:genetics*; D014960:X Chromosome / Q000032:analysis*
D015342:DNA Probes; D014128:Chromomycin A3