Increased UV sensitivity of Escherichia coli cells after introduction of foreign photolyase genes.
High-expression plasmids for photolyase (phr) genes from the bacteria Escherichia coli, Anacystis nidulans, Streptomyces griseus and Halobacterium halobium and the yeast Saccharomyces cerevisiae were constructed and introduced into E. coli phr recA cells. As previously reported, al introduced phr genes provided the host cells with photoreactivation-repair activity and the introduced E. coli phr gene rendered the host cells more UV-resistant in the dark. E. coli cells harboring foreign phr genes, however, were found to be more sensitive to UV light in the dark than cells containing the vector plasmid only. These differences in UV sensitivity in the dark disappeared when the host cells had an additional mutation, uvrA, suggesting that the foreign photolyases inhibited the E. coli excision-repair system.
Kobayashi
T
T
Research Institute for Tuberculosis and Cancer, Tohoku University, Sendai, Japan.
Takao
M
M
Oikawa
A
A
Yasui
A
A
D001483:Base Sequence; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000201:enzymology / Q000235:genetics; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000235:genetics* / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000528:radiation effects*; D005796:Genes; D006217:Halobacterium / Q000201:enzymology / Q000235:genetics; D008190:Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D013305:Streptomyces griseus / Q000201:enzymology / Q000235:genetics; D014466:Ultraviolet Rays
D015345:Oligonucleotide Probes; D008190:Lyases; D004255:Deoxyribodipyrimidine Photo-Lyase