title: The reaction specificities of the pea and a cyanobacterial thylakoid processing peptidase are similar but not identical.
abstract: The thylakoid processing peptidase from the cyanobacterium Phormidium laminosum has been extracted from thylakoid membranes by solubilization with Triton X-100. Its reaction specificity has been compared with the analogous pea peptidase by processing in vitro of radiolabelled wheat and P. laminosum thylakoid lumenal precursor polypeptides. The cyanobacterial polypeptide is processed to the mature size through an intermediate by the P. laminosum peptidase, but to a polypeptide that has a slightly greater apparent molecular weight than the intermediate by the pea peptidase. Both peptidases correctly process the wheat polypeptide. This suggests that the reaction specificities of the two peptidases are similar, but not identical.
authors.affiliation: Department of Biochemistry, University of Cambridge, UK.
authors.forename: T P
authors.initials: TP
authors.lastname: Wallace
authors.forename: C
authors.initials: C
authors.lastname: Robinson
authors.forename: C J
authors.initials: CJ
authors.lastname: Howe
mesh_terms: D000595:Amino Acid Sequence; D000458:Cyanobacteria / Q000201:enzymology*; D007887:Fabaceae; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000378:metabolism*; D010944:Plants / Q000201:enzymology*; D010946:Plants, Medicinal; D011498:Protein Precursors / Q000737:chemistry / Q000378:metabolism*; D021382:Protein Sorting Signals / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D008565:Membrane Proteins; D011498:Protein Precursors; D021382:Protein Sorting Signals; C021261:thylakoid polypeptides; D010447:Peptide Hydrolases