title: The PurR mutation of Drosophila melanogaster confers resistance to purine and 2,6-diaminopurine by elevating adenosine deaminase activity.
abstract: Media supplemented with purine (7H-imidazo[4,5-d]pyrimidine) or the purine analogue 2,6-diaminopurine (DAP) can be employed to select several classes of purine-resistant variants from mutagenized cultures of Drosophila. One class results in elevated resistance to purine and diaminopurine which is correlated with elevated activity of the enzyme adenosine deaminase (adenosine aminohydrolase = EC 3.5.4.4). The first member of this class, Pur R, maps to position 82 +/- in the right arm of the second chromosome. The Pur R mutation causes an elevation of adenosine deaminase (ADA) enzyme activity, apparently by altering a thermolabile, ADA-specific repressor. Pur R may thus encode a negative regulator of adenosine deaminase activity similar to the ADA-binding protein found in mammalian systems.
authors.affiliation: Department of Molecular and Cell Biology, University of Connecticut, Storrs 06269-2131.
authors.forename: F L
authors.initials: FL
authors.lastname: Dutton
authors.forename: A
authors.initials: A
authors.lastname: Chovnick
mesh_terms: D015075:2-Aminopurine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000225:Adenine / Q000031:analogs & derivatives*; D000243:Adenosine Deaminase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D008040:Genetic Linkage; D005838:Genotype; D006358:Hot Temperature; D008297:Male; D009154:Mutation; D009700:Nucleoside Deaminases / Q000378:metabolism*; D011687:Purines / Q000494:pharmacology*; D011995:Recombination, Genetic; D012097:Repressor Proteins / Q000378:metabolism
chemical_list: D011687:Purines; D012097:Repressor Proteins; D015075:2-Aminopurine; C007300:2,6-diaminopurine; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase; D000225:Adenine