Self heat shock and gamma delta T-cell reactivity.
We have investigated the effects of heat shock on T-cell induction and selection in vitro. We find that when cell preparations containing T lymphocytes are incubated for 30 min at 42 degrees C, a selective proliferation of gamma delta + T cells bearing the gamma delta T-cell antigen receptor follows. A greater enrichment of gamma delta + T cells is observed, upon preexposure to mycobacterial antigens in vivo. By comparing the effects of heat shock with that of mitogen or specific T-cell triggering by conventional antigens and by analyzing the gamma delta T-cell receptor genes expressed in cells that proliferate as a result of heat shock induction, we conclude that a subset of murine gamma delta T cells react to antigens on self cells in which a heat shock response was induced.
Rajasekar
R
R
Department of Medicine, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.
Sim
G K
GK
Augustin
A
A
D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006358:Hot Temperature; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007138:Immunoglobulin delta-Chains / Q000235:genetics*; D007146:Immunoglobulin lambda-Chains / Q000235:genetics*; D008168:Lung / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D007138:Immunoglobulin delta-Chains; D007146:Immunoglobulin lambda-Chains; D011948:Receptors, Antigen, T-Cell