Growth of Listeria monocytogenes at refrigeration temperatures.
The growth of three strains of Listeria monocytogenes at refrigeration temperatures (-0.5 to 9.3 degrees C) in chicken broth and/or UHT milk was determined using a rocking temperature gradient incubator. Minimum growth temperatures ranged from -0.1 to -0.4 degree C for the three strains. Lag times of 1-3 d and 3 to greater than 34 d were observed with incubation at 5 and 0 degrees C respectively. Corresponding generation times ranged from 13-24 h at 5 degrees C and 62-131 h at 0 degree C. The type of culture medium had an influence on both the rate and extent of growth. Incubation of cultures at 4 degrees C before inoculation caused a marked reduction in the lag time when compared with cultures which had been previously incubated at 30 degrees C.
Walker
S J
SJ
Campden Food and Drink Research Association, Chipping Campden, Glos., UK.
Archer
P
P
Banks
J G
JG
D000818:Animals; D003080:Cold Temperature; D003470:Culture Media; D005516:Food Microbiology; D006801:Humans; D008089:Listeria monocytogenes / Q000254:growth & development*; D008892:Milk / Q000382:microbiology; D012034:Refrigeration
D003470:Culture Media