Inhibition of cyclooxygenase-independent platelet aggregation by low vitamin E concentration.
Platelet aggregation induced by threshold concentrations of agonists such as collagen, PAF or epinephrine was inhibited in vitro by 100 microM aspirin but was restored by stimulating platelets with high concentrations of collagen, PAF or by a combination of epinephrine and PAF. Incubating aspirin-treated platelets with 50-100 microM vitamin E or vitamin E acetate inhibited platelet aggregation by high concentrations of collagen and PAF and by the combination of epinephrine and PAF; platelet thromboxane A2 formation was less than 10% in samples incubated with 100 microM aspirin. Apyrase, added to aspirin-treated platelet, did not influence platelet aggregation induced by epinephrine and PAF. The present study suggests that concentrations of vitamin E as low as 50-100 microM inhibit cyclooxygenase-independent platelet aggregation when combined with an inhibitor of the arachidonate pathway.
Violi
F
F
Institute of Clinica Medica I, University of Rome, Policlinico Umberto I, Italy.
Pratico
D
D
Ghiselli
A
A
Alessandri
C
C
Iuliano
L
L
Cordova
C
C
Balsano
F
F
D001081:Apyrase / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology; D001792:Blood Platelets / Q000378:metabolism; D003094:Collagen / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D006801:Humans; D010972:Platelet Activating Factor / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors; D011451:Prostaglandin-Endoperoxide Synthases / Q000494:pharmacology; D013928:Thromboxane A2 / Q000096:biosynthesis; D024505:Tocopherols; D014810:Vitamin E / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology*; D024502:alpha-Tocopherol / Q000031:analogs & derivatives*
D010972:Platelet Activating Factor; D010975:Platelet Aggregation Inhibitors; D014810:Vitamin E; D013928:Thromboxane A2; D003094:Collagen; D011451:Prostaglandin-Endoperoxide Synthases; D001081:Apyrase; D024502:alpha-Tocopherol; D024505:Tocopherols; D001241:Aspirin; D004837:Epinephrine