[Growth of the Yoshida ascites/hepatoma in co-culture with rat fibroblasts].
Ascites hepatoma cells (Y) co-cultured with rat fibroblasts (F) in Dulbecco-Eagle's MEM (DMEM) proliferate rapidly in suspension, at a rate consistent with that shown in vivo after intraperitoneal injection; the population doubling time is about 1 day. The log phase of growth may be retained indefinitely, provided fresh medium is supplied regularly and the F monolayer is changed when necessary. The tumorigenicity is preserved. To maintain a high rate of growth the presence of F seems important: in this study, culturing without F in various media at best only sustained slow proliferation rates; this is in keeping with the notion of normal tissue components supplying useful factors to the neoplastic cells. Adding minced polyester surgical thread (Mersilene - M) into the co-cultures slowed down the growth of Y to some extent, yet no evidence has been obtained of toxic compounds released by M.
Melandri
P
P
Istituto di Patologia Generale dell'Università di Ferrara.
Benassi
G
G
Ventrelli
I
I
D000818:Animals; D002450:Cell Communication; D002455:Cell Division; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology*; D006133:Growth Substances / Q000502:physiology; D010795:Phthalic Acids; D011092:Polyethylene Glycols; D011093:Polyethylene Terephthalates; D051381:Rats; D012517:Sarcoma, Yoshida / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000473:pathology
D006133:Growth Substances; D010795:Phthalic Acids; D011093:Polyethylene Terephthalates; C025539:Lavsan; D011092:Polyethylene Glycols
Osservazioni sulla crescita dell'epatoma--ascite di Yoshida in cocoltura con fibroblasti di ratto.