[Virus-specific proteins and RNA of the virus of hemorrhagic fever with renal syndrome].
Virus-specific proteins G1, G2, and N with molecular weights of 70, 55-57, and 50 kilodaltons, respectively, were detected by radioimmunodiffusion tests in VERO E-6 cells infected with strains of virus of hemorrhagic fever with renal syndrome (HFRS) isolated in the European USSR from a patient with HFRS, a fatal human case of HFRS (the strains K-27 and P-360) and from a bank vole (strain CG-1820). The sera from human convalescents after HFRS in the European USSR and rat sera prepared with the CG-1820 strain precipitated proteins possessing similar electrophoretic characteristics from a lysate of cells infected with the CG-1820, K-27 and P-360 strains. The sera from human HFRS convalescents in the Far East did not precipitate protein Gl. The viral RNA derived by immunosorption method from intracellular nucleocapsids of CG-1820 strain and strain 4590 (isolated from Ap. Peninsulae in the Far East) contained 3 classes of molecules: L, M, and S. L- and M-RNA of these strains had the same molecular weight (1.62 and 1.38 megadaltons). The molecular weight of S-RNA of the strain 4590 was 0.76 megadalton and that of the CG-1820 strain 0.83 megadalton. It is assumed that there are species differences among the viruses, causative agents of HFRS, circulating in the European and Far East regions of the USSR.
Lukashevich
I S
IS
Tkachenko
E A
EA
Lemeshko
N N
NN
Rezapkin
G V
GV
Stel'makh
T A
TA
Pashkov
A Ia
AIa
Vladyko
A S
AS
Dzagurova
T K
TK
Shkolina
T V
TV
Scheslenok
E P
EP
D000818:Animals; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D006476:Orthohantavirus / Q000032:analysis*; D006801:Humans; D012367:RNA, Viral / Q000032:analysis*; D015531:Radioimmunoprecipitation Assay; D014764:Viral Proteins / Q000032:analysis*
D012367:RNA, Viral; D014764:Viral Proteins
Virusspetsificheskie belki i RNK virusa gemorragicheskoĭ lokhoradki s pochechnym sindromom.