[Diagnostic importance of HDL cholesterol determination].
The present paper describes the sensitivity to quantification of changes of HDL-cholesterol in serum by two different precipitation and analytical techniques during the treatment of patients. After the precipitation of VLDL and LDL by phosphotungstic acid/magnesium chloride the chemical determination of HDL-cholesterol in serum with the Liebermann-Burchard reaction yields different results in comparison to enzymatic HDL-cholesterol determined in serum supernatant after the precipitation by polyethylene glycol 20.000. Correlation analyses of apolipoprotein A-I with enzymatic HDL-, HDL2-, HDL3-cholesterol or electrophoretic alpha-cholesterol demonstrate that the therapeutically induced changes (by training and diet) of lipid composition are more correctly reflected by the enzymatic determination of HDL-cholesterol after serum precipitation by polyethylene glycol.
Reissner
J
J
Zentrallabor, Bezirkskrankenhauses Meiningen.
Herrmann
W
W
D008076:Cholesterol, HDL / Q000097:blood*; D015444:Exercise; D006801:Humans; D006937:Hypercholesterolemia / Q000178:diet therapy / Q000628:therapy*; D008297:Male; D008875:Middle Aged
D008076:Cholesterol, HDL
Diagnostische Wertigkeit der HDL-Cholesterol-Bestimmung.