title: Purification of human C-reactive protein by immunoaffinity chromatography using mouse monoclonal antibody.
abstract: Human C-reactive protein (CRP) was purified from sera with a high concentration of CRP by immunoaffinity chromatography using mouse monoclonal antibody (mAb) to CRP (No. 18) and ion exchange on DEAE-Sephacel. CRP bound to the immunoaffinity column was eluted by 0.5 M acetate buffer, pH 5.0 containing 1.5 M NaCl. Final recovery of CRP was 90%. No significant immunochemical differences were observed between CRP obtained by the immunoaffinity chromatography and CRP purified by the method of Hokama and Riley.
authors.affiliation: Tumour Laboratory, Tokyo, Japan.
authors.forename: W
authors.initials: W
authors.lastname: Nunomura
authors.forename: M
authors.initials: M
authors.lastname: Hatakeyama
authors.forename: H
authors.initials: H
authors.lastname: Hirai
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002097:C-Reactive Protein / Q000276:immunology / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D005779:Immunodiffusion; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal; D002097:C-Reactive Protein