Antigen-induced T-cell changes: modulation by pharmacologic agents.
To determine the effect of pharmacologic modulation of alterations of peripheral blood T-cell subsets caused by antigen-induced bronchoconstriction, we administered albuterol immediately after antigen-induced bronchoconstriction in a double-blind to protocol to 12 atopic asthmatic subjects. We also administered cromolyn sodium before antigen to 7 of the same subjects. Peripheral blood T-cell subset composition (CD4, CD8, Ia) of a highly purified T-cell preparation was determined before, 24, 48, 72, and 168 h after bronchoconstriction. We found that placebo inhalation immediately after antigen-induced bronchoconstriction did not affect subsequent peripheral blood T-cell subset changes (decrease in CD4+ and increase in Ia+ T lymphocytes). In contrast, inhaled albuterol abolished these T-cell subset changes. Although cromolyn sodium significantly decreased the severity of antigen-induced bronchoconstriction, it did not affect T-cell subset composition changes at the dosage used. We conclude that albuterol can ablate T-cell subset changes associated with antigen-induced bronchoconstriction. Cromolyn sodium ameliorates bronchoconstriction, but has no affect on T-cell subset composition changes. This implies that T-cell changes and bronchoconstriction caused by antigen inhalation are mediated through different pathways.
Varghese
J
J
Department of Medicine, Case Western Reserve School of Medicine, Cleveland, Ohio.
Gerblich
A
A
Salik
H
H
Schuyler
M
M
D000280:Administration, Inhalation; D000420:Albuterol / Q000008:administration & dosage / Q000494:pharmacology*; D000941:Antigens / Q000276:immunology*; D001249:Asthma / Q000097:blood / Q000276:immunology / Q000503:physiopathology; D001980:Bronchi / Q000187:drug effects / Q000503:physiopathology; D001985:Bronchial Provocation Tests; D003251:Constriction, Pathologic / Q000209:etiology / Q000517:prevention & control; D004205:Cromolyn Sodium / Q000008:administration & dosage / Q000494:pharmacology*; D004311:Double-Blind Method; D005541:Forced Expiratory Volume / Q000187:drug effects; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism; D007958:Leukocyte Count / Q000187:drug effects*; D013601:T-Lymphocytes / Q000276:immunology*
D000941:Antigens; D007073:Immunoglobulin E; D004205:Cromolyn Sodium; D000420:Albuterol