title: Rhodopsin expressed in Chinese hamster ovary cells regulates adenylyl cyclase activity.
abstract: The cDNA encoding bovine opsin was transfected into Chinese hamster ovary (CHO) cells to generate stable clones expressing the rod cell photoreceptor protein. Cells expressing opsin, when incubated in 11-cis retinal and exposed to light, inhibited forskolin-stimulated adenylyl cyclase activity. Rhodopsin-mediated inhibition of adenylyl cyclase was prevented by treatment of cells with pertussis toxin. In the same cells, thrombin stimulated phosphatidylinositol hydrolysis through G protein-mediated pathways, but rhodopsin neither significantly influenced the action of thrombin nor stimulated phosphatidylinositol hydrolysis. Our findings indicate that rhodopsin selectively regulates a Gi protein in intact CHO cells that is coupled to adenylyl cyclase but not to phospholipase C.
authors.affiliation: National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206.
authors.forename: E R
authors.initials: ER
authors.lastname: Weiss
authors.forename: R A
authors.initials: RA
authors.lastname: Heller-Harrison
authors.forename: E
authors.initials: E
authors.lastname: Diez
authors.forename: M
authors.initials: M
authors.lastname: Crasnier
authors.forename: C C
authors.initials: CC
authors.lastname: Malbon
authors.forename: G L
authors.initials: GL
authors.lastname: Johnson
mesh_terms: D037361:Adenylate Cyclase Toxin; D000067956:Adenylyl Cyclase Inhibitors; D000818:Animals; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D005260:Female; D019204:GTP-Binding Proteins / Q000502:physiology; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D008027:Light; D010053:Ovary; D037342:Pertussis Toxin; D010716:Phosphatidylinositols / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology*; D012168:Retinal Pigments / Q000494:pharmacology*; D012172:Retinaldehyde / Q000494:pharmacology; D012243:Rhodopsin / Q000235:genetics / Q000494:pharmacology*; D013873:Thionucleotides / Q000378:metabolism; D013917:Thrombin / Q000494:pharmacology; D014162:Transfection; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
chemical_list: D037361:Adenylate Cyclase Toxin; D000067956:Adenylyl Cyclase Inhibitors; D010716:Phosphatidylinositols; D011994:Recombinant Proteins; D012168:Retinal Pigments; D013873:Thionucleotides; D010566:Virulence Factors, Bordetella; D005576:Colforsin; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate; D004247:DNA; D012243:Rhodopsin; D037342:Pertussis Toxin; D013917:Thrombin; D019204:GTP-Binding Proteins; D012172:Retinaldehyde