Localization of phosphoserine residues in the alpha subunit of rabbit skeletal muscle phosphorylase kinase.
The alpha subunit of skeletal muscle phosphorylase kinase, as isolated, carries phosphate at the serine residues 1018, 1020 and 1023. Employing the S-ethyl-cysteine method, these residues are found to be phosphorylated partially, i.e. differently phosphorylated species exist in muscle. Serine 1018 is a site which can be phosphorylated by the cyclic-AMP-dependent protein kinase. The serine residues 972, 985 and 1007 are phosphorylated by phosphorylase kinase itself when its activity is stimulated by micromolar concentrations of Ca2+. These phosphorylation sites are not identical to those found to be phosphorylated already in the enzyme as prepared from freshly excised muscle. A 'multiphosphorylation loop' uniquely present in this but not in the homologous beta subunit contains all the phosphoserine residues so far identified in the alpha subunit.
Meyer
H E
HE
Abteilung für Biochemie Supramolekularer Systeme, Ruhr-Universität Bochum, Federal Republic of Germany.
Meyer
G F
GF
Dirks
H
H
Heilmeyer
L M
LM
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000302:isolation & purification; D000818:Animals; D001665:Binding Sites; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology*; D010446:Peptide Fragments / Q000302:isolation & purification; D010447:Peptide Hydrolases; D010710:Phosphates / Q000302:isolation & purification; D010764:Phosphorylase Kinase / Q000302:isolation & purification*; D010766:Phosphorylation; D010768:Phosphoserine / Q000032:analysis*; D011817:Rabbits; D012694:Serine / Q000031:analogs & derivatives*
D000596:Amino Acids; D010446:Peptide Fragments; D010710:Phosphates; D010768:Phosphoserine; D012694:Serine; D010764:Phosphorylase Kinase; D010447:Peptide Hydrolases