[The lymphocyte transformation test using mononuclear cells of elderly humans as a suitable in vitro method for testing of structure activity relationships of splenin peptides].
More and more new immunostimulatory substances are developed. Besides old and new drugs have to be examined with regard to their side effects. Hence it follows demands for effective test possibilities. The lymphocyte transformation test with mononuclear cells of old men is a suitable in vitro method for testing of substances with thymic hormone-like activity in order to investigate the structure-effect-relationships. Thymopentin, human splenopentin and human diacetyl-splenopentin increase the phytohaemagglutinin induced lymphocyte transformation, on the other hand in the case of bovine splenopentin and human splenotritin this effect was not observed. By means of a patient with hypogammaglobulinaemia is demonstrated that the lymphocyte transformation test could also be a suitable method for the estimation of pharmacodynamics such peptides after in vivo treatment.
Simon
H U
HU
Institut für Klinische Immunologie, Friedrich-Schiller-Universität Jena.
Forner
K
K
Büttner
A
A
Haroske
D
D
Vogt
K H
KH
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000368:Aged; D000369:Aged, 80 and over; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects*; D009504:Neutrophils / Q000187:drug effects*; D010455:Peptides / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D013223:Statistics as Topic; D013329:Structure-Activity Relationship; D013946:Thymopoietins / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D010455:Peptides; D010835:Phytohemagglutinins; D013946:Thymopoietins; C032841:thymopoietin III
Der Lymphozytentransformationstest unter Verwendung von mononukleären Zellen alter Menschen als geeignete in vitro-Methode zur Testung von Struktur-Wirkungs-Beziehungen von Splenin-Peptiden.