In vivo activation by ultraviolet rays of the human immunodeficiency virus type 1 long terminal repeat.
It has been previously shown in vitro that the human immunodeficiency virus type 1 long terminal repeat (LTR) is activated by ultraviolet irradiation. In order to analyze if a similar effect could occur in vivo, transgenic mice carrying the lacZ gene under the control of the viral LTR were irradiated at 280-300 and 254 nm. These mice spontaneously expressed the transgene in the epidermis and the lens of both adults and embryos. Irradiations caused a significant increase in skin beta-galactosidase activity. This phenomenon might be involved in viral activation and could be of interest in regard to the skin pathology observed during an HIV infection.
Cavard
C
C
Unité 152 Institut Cochin de Génétique Moléculaire, Institut National de la Santé et de la Recherche Medical, Paris, France.
Zider
A
A
Vernet
M
M
Bennoun
M
M
Saragosti
S
S
Grimber
G
G
Briand
P
P
D000818:Animals; D015967:Gene Expression Regulation, Viral / Q000528:radiation effects*; D016325:HIV Long Terminal Repeat / Q000528:radiation effects*; D006801:Humans; D007763:Lac Operon; D051379:Mice; D008822:Mice, Transgenic; D012867:Skin / Q000382:microbiology; D014466:Ultraviolet Rays; D001616:beta-Galactosidase / Q000032:analysis
D001616:beta-Galactosidase