Degradation of fibrinogen by tissue plasminogen activator. Consequences for fibrin polymerization.
In patients treated with tissue plasminogen activator (t-PA), there was a marked increase in concentration of fibrinopeptide A (fpA). The purpose of this study was to analyse the activation of coagulation in plasma treated in vitro by pharmacological doses of t-PA. From our results, it appears that fpA release due to direct interaction of fibrinogen with t-PA, in the absence of plasminogen, is very low and not observed with pharmacological doses of t-PA. In contrast in the presence of plasminogen, the release of fpA is much higher and induces fibrin formation concomitantly to fibrinogen degradation.
Mirshahi
M
M
Laboratoire d'Hématologie, INSERM U 150, Hopital Lariboisière, Paris.
Soria
J
J
Soria
C
C
Samama
M
M
Caen
J P
JP
D001704:Biopolymers; D015153:Blotting, Western; D005337:Fibrin / Q000096:biosynthesis*; D005340:Fibrinogen / Q000187:drug effects / Q000378:metabolism*; D005344:Fibrinopeptide A / Q000378:metabolism; D006801:Humans; D046911:Macromolecular Substances; D010959:Tissue Plasminogen Activator / Q000494:pharmacology*
D001704:Biopolymers; D046911:Macromolecular Substances; D005344:Fibrinopeptide A; D005337:Fibrin; D005340:Fibrinogen; D010959:Tissue Plasminogen Activator