title: Intestinal glucoamylase & other disaccharidases in children with protracted diarrhoea.
abstract: Brush border lactase, sucrase and glucoamylase activities were assessed in jejunal mucosal biopsy specimens from 34 children (median age 11 months; range 1.5-38) having protracted diarrhoea with failure to thrive and 8 well nourished children with normal jejunal mucosal histology (median age 10.2 months; range 2-37). All enzymes showed progressive decrease in activity which was directly in relation to increasing degree of mucosal injury (P less than 0.002). Lactase was significantly reduced even in patients with protracted diarrhoea and normal mucosa (P less than 0.05). Glucoamylase and sucrase were significantly reduced only in the presence of mucosal injury (P less than 0.01). Our data suggest that most children with protracted diarrhoea may not tolerate lactose containing feeds and may need lactose-free diets preferably based on starch. A small number of children with protracted diarrhoea, who have severe mucosal injury may not be able to handle even starch and may require diets based on short chain glucose polymers. The findings of this study, need to be corroborated with well-controlled metabolic balance studies.
authors.affiliation: Department of Paediatrics, All India Institute of Medical Sciences, New Delhi.
authors.forename: V
authors.initials: V
authors.lastname: Khoshoo
authors.forename: M K
authors.initials: MK
authors.lastname: Bhan
authors.forename: R
authors.initials: R
authors.lastname: Jain
authors.forename: S
authors.initials: S
authors.lastname: Jayashree
authors.forename: N
authors.initials: N
authors.lastname: Bhandari
authors.forename: S
authors.initials: S
authors.lastname: Sazawal
authors.forename: M
authors.initials: M
authors.lastname: Mathur
mesh_terms: D002675:Child, Preschool; D003968:Diarrhea, Infantile / Q000201:enzymology*; D005696:Galactosidases / Q000378:metabolism*; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism*; D006801:Humans; D007223:Infant; D007413:Intestinal Mucosa / Q000201:enzymology; D007583:Jejunum / Q000201:enzymology*; D008871:Microvilli / Q000201:enzymology; D013393:Sucrase / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D005696:Galactosidases; D001616:beta-Galactosidase; D005087:Glucan 1,4-alpha-Glucosidase; D013393:Sucrase