The occurrence of alpha (1----2) linked N-acetylperosamine--homopolymer in lipopolysaccharides of non-O1 Vibrio cholerae possessing an antigenic factor in common with O1 V. cholerae.
Chemical analysis was carried out on lipopolysaccharides from Vibrio cholerae bio-serogroup Hakata 487-85. The O-specific chain of the phenol-soluble lipopolysaccharides was demonstrated by 13C-NMR spectroscopy and methylation analysis to contain a linear homopolymer of alpha(1----2) linked N-acetylperosamine (4-acetamido-4,6-dideoxy-D-mannopyranose), which was closely similar to but not identical to a linear alpha(1----2) linked N-3-deoxy-L-glycerotetronyl (S-2,4-dihydroxybutyryl) perosamine-homopolymer constituting that of O1 Vibrio cholerae lipopolysaccharides.
Haishima
Y
Y
Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Saitama.
Kondo
S
S
Hisatsune
K
K
D000606:Amino Sugars / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D011134:Polysaccharides / Q000737:chemistry*; D014734:Vibrio cholerae / Q000032:analysis*
D000606:Amino Sugars; D008070:Lipopolysaccharides; D011134:Polysaccharides; C069468:N-acetylperosamine