In vivo induction of Ia expression in murine cornea after intravitreal injection of interferon-gamma.
Intravitreal injection of interferon gamma (IFN-gamma) induces increased expression of Class II major histocompatibility complex (Ia) antigen expression on corneal endothelial cells and stromal fibroblasts. In contrast, IFN-gamma has no detectable effect on Ia antigen expression in epithelium. Induction of Ia antigen expression was rapid with increases detectable as early as 6-12 hours after a single injection of 1 x 10(5) units. Expression peaked at 24-48 hours and decreased to background levels by 120 hours. The Ia antigen expression increased in a dose-dependent manner, and IFN-gamma treatment also induced the synthesis of increased amounts of a 65-kilodalton (kD) protein in the cornea. Increased levels of this 65-kd protein are seen as early as 12 hours after treatment and can be induced with as little as 1 x 10(2) units of IFN-gamma. The function of the 65-kd protein is unknown. This model should be useful in studies on in vivo modulation of Ia antigen expression.
Brandt
C R
CR
Department of Ophthalmology, University of Wisconsin-Madison.
Knupfer
P B
PB
Boush
G A
GA
Gausas
R E
RE
Chandler
J W
JW
D000818:Animals; D003315:Cornea / Q000187:drug effects / Q000276:immunology*; D003319:Corneal Stroma / Q000187:drug effects / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004728:Endothelium, Corneal / Q000187:drug effects / Q000276:immunology; D005136:Eye Proteins / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis*; D007371:Interferon-gamma / Q000494:pharmacology* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins; D014822:Vitreous Body
D005136:Eye Proteins; D000949:Histocompatibility Antigens Class II; D011994:Recombinant Proteins; D007371:Interferon-gamma