Biochemical analysis of normal and osteoarthritic human cartilage.
Non-collagenous proteins from the articular cartilage of normal subjects and patients with degenerative joint disease were extracted sequentially. Proteoglycans and the other glycoproteins were more extractable from the osteoarthritic cartilage at lower ionic strength than those from the normal cartilage. A 50-kD protein which seems specific to osteoarthritic cartilage was identified. Three different populations of proteoglycans were purified from normal and only two from osteoarthritic cartilage. Moreover, greater amounts of albumin and fibronectin were found in the pathological cartilage. No differences were observed between link proteins from normal and osteoarthritic cartilage, nor in their molecular weight or the amounts extracted.
Vilamitjana-Amédée
J
J
INSERM U306, Université de Bordeaux II, France.
Harmand
M F
MF
D015153:Blotting, Western; D002356:Cartilage / Q000737:chemistry*; D003094:Collagen / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D008970:Molecular Weight; D009994:Osmolar Concentration; D010003:Osteoarthritis / Q000378:metabolism*; D011506:Proteins / Q000032:analysis; D011509:Proteoglycans / Q000032:analysis; D000099249:Proteoglycan Link Protein
D003094:Collagen; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D011506:Proteins; D011509:Proteoglycans; D000099249:Proteoglycan Link Protein