Effect of fructose on glycogen synthesis in the perfused rat liver.
The effect of fructose on glycogen synthesis was examined in the perfused liver of starved rats. With increasing fructose concentration in the perfusate, glycogen synthesis and the % a form of glycogen synthase increased to a maximum at 2 mM and then decreased, progressively. The glucose 6-P level increased with the increase in fructose concentration. On the other hand, the ATP content was unchanged at a concentration of 2 mM or less and decreased at 3 mM or more. We also showed that the stimulation of glycogen synthesis by fructose at a concentration of 2 mM or less was due to activation of glycogen synthase by accumulated glucose 6-P and that ATP depletion at a concentration of 3 mM or more caused an increase in phosphorylase a and a decrease in glycogen synthase activity even in the presence of a high concentration of glucose 6-P.
Nishi
T
T
Second Department of Surgery, Osaka University Medical School, Japan.
Kido
Y
Y
Ogawa
A
A
Furuya
E
E
Mori
T
T
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005632:Fructose / Q000494:pharmacology*; D005958:Glucosephosphates / Q000378:metabolism; D006006:Glycogen Synthase / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008112:Liver Glycogen / Q000096:biosynthesis*; D008297:Male; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D014530:Uridine Diphosphate / Q000032:analysis
D005958:Glucosephosphates; D008112:Liver Glycogen; D005632:Fructose; D014530:Uridine Diphosphate; D000255:Adenosine Triphosphate; D006006:Glycogen Synthase