Imaging cells with the atomic force microscope.
Different types of cells have been imaged with the atomic force microscope. The morphology of the archaebacterium Halobacterium halobium in its dry state was revealed. On a leaf of the small Indian tree Lagerstroemia subcostata a stoma was imaged. The lower side of a water lily leaf was imaged in water showing features down to 12 nm. Finally, fixed red and white blood cells were imaged in buffer showing features down to 8 nm. The images demonstrate that atomic force microscopy can provide high-resolution images of cell surfaces under physiological conditions.
Butt
H J
HJ
Department of Physics, University of California, Santa Barbara 93106.
Wolff
E K
EK
Gould
S A
SA
Dixon Northern
B
B
Peterson
C M
CM
Hansma
P K
PK
D000818:Animals; D004912:Erythrocytes / Q000648:ultrastructure*; D006217:Halobacterium / Q000648:ultrastructure*; D008214:Lymphocytes / Q000648:ultrastructure*; D008853:Microscopy / Q000379:methods*; D010944:Plants / Q000648:ultrastructure*