31P-NMR assay of phosphatidylcholine and phosphatidylethanolamine in AL721.
A 31P-NMR based method has been developed for the assay of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) in AL721. The assay is based on the comparison of NMR signal intensities of the phosphatidyl moiety of PC and PE to the signal of triphenylphosphate (TPP), the internal standard. The assay is specific and reliable for the quantitation of phospholipid mixtures. Its uncertainty, due primarily to low intensity of 31P signals, was estimated at less than 6%.
Olson
L L
LL
Life Sciences Division, SRI International, Menlo Park, CA 94025.
Cheung
A P
AP
D004338:Drug Combinations; D005989:Glycerides / Q000032:analysis*; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D010755:Organophosphates; D009943:Organophosphorus Compounds / Q000737:chemistry; D010713:Phosphatidylcholines / Q000032:analysis*; D010714:Phosphatidylethanolamines / Q000032:analysis*; D010759:Phosphorus Isotopes; D012015:Reference Standards
D004338:Drug Combinations; D005989:Glycerides; D007202:Indicators and Reagents; D010755:Organophosphates; D009943:Organophosphorus Compounds; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010759:Phosphorus Isotopes; C046637:neutral lipid, phosphatidylcholine, phosphatidylethanolamine drug combination; C005445:triphenyl phosphate