STB1, a mouse lymphocyte marker found on T cell and B cell subpopulations.
A set of monoclonal antibodies has been raised to cell surface markers which are either hyperexpressed, or exclusive to activated murine lymphocytes. One antigen is present on all B cells of the Ly1 lineage and defines a novel subpopulation of classical B cells, most of which are activated B cells. In Western blots, this antigen appears as a single glycoprotein of 95 kDa molecular weight but of greater interest, is its expression at high levels on all mature peripheral T cells but on only 30% of thymocytes. This marker is expressed at high levels on all CD4 and CD8 thymocytes, most double-negative (CD4-CD8-) thymocytes, and at low levels on double-positive (CD4+CD8+) thymocytes. It may prove useful as a developmental marker for subdividing the double-positive (CD4+CD8+) and double negative (CD4-CD8-) thymocyte populations. Taken together these results indicate that mab 3.17 recognises a novel lymphocyte antigen expressed on subsets of T and B cells, which is hyperexpressed on lymphocyte activation.
Andrew
D
D
Department of Zoology and Cell Biology, University College, London.
Jayakumar
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D015415:Biomarkers / Q000032:analysis*; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens; D002461:Cell Line, Transformed; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1; D051379:Mice; D008807:Mice, Inbred BALB C; D016028:Receptors, Leukocyte-Adhesion / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D015415:Biomarkers; D015704:CD4 Antigens; D016827:CD8 Antigens; D016169:Lymphocyte Function-Associated Antigen-1; D016028:Receptors, Leukocyte-Adhesion