Androgen-dependent protein from mouse vas deferens. cDNA cloning and protein homology with the aldo-keto reductase superfamily.
We report the cloning and sequencing of a 1225-base pair cDNA encoding an abundant protein from mouse vas deferens. An open reading frame of 948 nucleotides encodes a protein of 316 amino acids with a calculated mass of 35,965 Da. A high degree of homology was found between this protein and members of the aldo-keto reductase superfamily, especially aldose reductase from human placenta (82%) and from rat lens (80%), suggesting that it could be an aldose reductase. Castration resulted in a marked decrease in the level of the 1.4 kilobase mRNA coding for the 35,965 protein, whereas administration of testosterone to castrated males resulted in a marked increase. Southern hybridization analysis of mouse genomic DNA revealed relatively simple patterns of bands.
Pailhoux
E A
EA
Physiologie Comparée et Endocrinologie, Centre National de la Recherche Scientifique U.A. 360, Université Blaise Pascal, Les Cézeaux, Aubière France.
Martinez
A
A
Veyssiere
G M
GM
Jean
C G
CG
D000449:Aldehyde Reductase / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D006801:Humans; D007908:Lens, Crystalline / Q000201:enzymology; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009919:Orchiectomy; D010920:Placenta / Q000201:enzymology; D011506:Proteins / Q000235:genetics*; D051381:Rats; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013739:Testosterone / Q000494:pharmacology*; D014649:Vas Deferens / Q000737:chemistry*
D011506:Proteins; D013739:Testosterone; D004247:DNA; C066498:Akr1b7 protein, mouse; D000449:Aldehyde Reductase