title: Establishment and characterization of acute B-cell lymphocytic leukemia cell line showing (8;14) and (14;18) chromosome translocation.
abstract: Cell line KHM-2B expressing two oncogene products, c-myc and bcl-2, was established from a patient with acute lymphocytic leukemia with an 8;14 and 14;18 chromosome translocation. Surface marker studies of the cell line showed that the cells were positive for HLA-DR, CALLA (CD10), B1 (CD20) and B4 (CD19), but negative for T11 (CD2). The fresh cells from peripheral blood of the patient had no surface immunoglobulins, whereas KHM-2B cells were positive for mu.lambda type surface immunoglobulin. A cytogenetic analysis of the cell line revealed two translocations, t (8;14) (q24;q32) and t(14;18)(q32;q21). Rearrangement of the c-myc and bcl-2 genes was detected by Southern blot analysis of the KHM-2B DNA. Northern blot analysis revealed production of c-myc and bcl-2 mRNAs. These results indicated that two oncogenes were activated by two translocations to immunoglobulin genes.
authors.affiliation: Second Department of Internal Medicine, Kumamoto University Medical School, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Matsuzaki
authors.forename: H
authors.initials: H
authors.lastname: Hata
authors.forename: N
authors.initials: N
authors.lastname: Asou
authors.forename: H
authors.initials: H
authors.lastname: Suzushima
authors.forename: Y
authors.initials: Y
authors.lastname: Akahoshi
authors.forename: M
authors.initials: M
authors.lastname: Yoshida
authors.forename: S
authors.initials: S
authors.lastname: Nagakura
authors.forename: T
authors.initials: T
authors.lastname: Ishii
authors.forename: I
authors.initials: I
authors.lastname: Sanada
authors.forename: K
authors.initials: K
authors.lastname: Takatsuki
mesh_terms: D000954:Antigens, Surface / Q000032:analysis; D015152:Blotting, Northern; D015139:Blotting, Southern; D002051:Burkitt Lymphoma / Q000235:genetics* / Q000473:pathology; D002883:Chromosomes, Human, Pair 14; D002887:Chromosomes, Human, Pair 18; D002898:Chromosomes, Human, Pair 8; D006801:Humans; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D000954:Antigens, Surface