Effect of praziquantel versus hycanthone on deoxyribonucleic acid content of hepatocytes in murine schistosomiasis mansoni.
Cytophotometric measurement of the effect of praziquantel (500 mg/kg for 2 days) versus hycanthone (60 mg/kg for 3 days) on hepatocyte nuclear deoxyribonucleic acid (DNA) content was evaluated in Schistosoma mansoni infected mice. Drugs were given 8 weeks post-infection and repeated weekly for 4 weeks. DNA values of infected untreated control and infected drug treated groups were related to the median and upper diploid DNA values of normal control. Schistosoma mansoni infection per se did not change the hepatocyte DNA content, yet aneuploidy was 16.7%. Praziquantel did not result in significant change of DNA content or ploidy, while hycanthone resulted in marked significant increase of DNA content (328.9%) and aneuploidy (100%), compared to infected untreated control. Histopathological examination revealed hyperchromatic nuclei with mitosis in the hepatocytes of hycanthone treated mice, but not in praziquantel treated animals. These DNA changes were found to correlate with the reported safety of praziquantel and the carcinogenicity of hycanthone.
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Pharmacology Department, Theodor Bilharz Research Institute, Imbaba, Guiza, Egypt.
D000818:Animals; D002273:Carcinogens; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000378:metabolism*; D006826:Hycanthone / Q000494:pharmacology* / Q000633:toxicity; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D011223:Praziquantel / Q000494:pharmacology* / Q000633:toxicity; D012555:Schistosomiasis mansoni / Q000378:metabolism*; D013892:Thioxanthenes / Q000494:pharmacology*
D002273:Carcinogens; D013892:Thioxanthenes; D006826:Hycanthone; D011223:Praziquantel; D004247:DNA