Dihydropteridine reductase activity in eluates from dried blood spots: automation of an assay for a national screening service.
We describe the automation, using a Cobas Bio centrifugal analyser, of a method for dihydropteridine reductase (DHPR) assay in eluates from dried blood spots. This is used as part of a routine screening service of neonates with hyperphenylalaninaemia in the United Kingdom. Automation reduced reagent volumes and analysis time by about 80% and improved the precision of the assay. Relating DHPR activity to haemoglobin concentration of the eluate further improved the precision of the assay and removed some differences in 'apparent' DHPR activity between samples from different countries and different age groups.
Surplice
I M
IM
Clinical Chemistry Department, Children's Hospital, Birmingham, UK.
Griffiths
P D
PD
Green
A
A
Leeming
R J
RJ
D000367:Age Factors; D001331:Automation / Q000295:instrumentation*; D001801:Blood Stains; D002498:Centrifugation / Q000379:methods*; D004093:Dihydropteridine Reductase / Q000032:analysis*; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D008403:Mass Screening / Q000295:instrumentation*; D009247:NADH, NADPH Oxidoreductases / Q000032:analysis*; D010661:Phenylketonurias / Q000175:diagnosis / Q000201:enzymology; D013222:State Medicine; D006113:United Kingdom
D006454:Hemoglobins; D004093:Dihydropteridine Reductase; D009247:NADH, NADPH Oxidoreductases