Regulation of Ig gene expression in normal lymphocytes. I. The half-life of secreted mu chain mRNA differs from that of membrane mu chain mRNA in resting and activated B cells.
Resting and activated B lymphocytes were used to study the stability of mu-specific precursor and mature mRNA. Resting cells which predominantly process the mu precursor towards micron do so rather slowly as reflected in a precursor half-life (T1/2) of 1-2 h. The small amount of secreted mu chain mRNA is fairly stable (T1/2 approximately 8 h) compared to membrane mu chain (T1/2 approximately 4 h). After activation the precursor processing is very fast (T1/2 approximately 10 min) and the stability of mu2, which now predominates, increases (T1/2 approximately 16 h) while the half-life of micron remains at about 4 h. The data indicate that normal B cells regulate mu-specific mRNA stability differently from tumor cells of the B cell lineage.
Berberich
I
I
Institut für Virologie und Immunbiologie, Universität Würzburg, FRG.
Schimpl
A
A
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D015870:Gene Expression; D005803:Genes, Immunoglobulin; D007148:Immunoglobulin mu-Chains / Q000235:genetics*; D008213:Lymphocyte Activation; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D012322:RNA Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D012260:Ribonucleases / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D007148:Immunoglobulin mu-Chains; D008565:Membrane Proteins; D012322:RNA Precursors; D012333:RNA, Messenger; D012260:Ribonucleases