[Production and characterization of a high affinity monoclonal antibody with digoxin and digitoxin specificity].
A monoclonal antibody with a high affinity for digoxin (KA = 5.5 x 10(10) M-1) and digitoxin KA = 5.0 x 10(10) M-1) was produced by somatic cell fusion. This antibody, designated 2A3, displayed little cross reactivity with other glucosides and no cross reactivity with endogenous steroids. It was shown that 2A3 is a suitable tool in an enzyme immunoassay for digoxin and digitoxin.
Simon
H U
HU
Institut für Klinische Immunologie, Friedrich-Schiller-Universität Jena.
Hubl
W
W
Müller
W D
WD
Wolf
I
I
Schlenvoigt
G
G
Jäger
L
L
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D003429:Cross Reactions; D004074:Digitoxin / Q000276:immunology*; D004077:Digoxin / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay
D000911:Antibodies, Monoclonal; D004077:Digoxin; D004074:Digitoxin
Produktion und Charakterisierung eines hochaffinen monoklonalen Antikörpers mit Digoxin- und Digitoxin-Spezifität.