Zinc increases EGF-stimulated DNA synthesis in primary mouse hepatocytes. Studies in tumor promoter-treated cell cultures.
To investigate factors influencing cell proliferation, cells are often cultured in serum-free medium. In the present study it is shown that addition of zinc chloride (40 microM) to primary mouse hepatocytes, cultured in Dulbecco's minimal essential medium, markedly enhanced growth factor (EGF)-stimulated [3H]thymidine incorporation into DNA. Treatment of cell cultures with phenobarbital or 3,4,3',4'-tetrachlorobiphenyl (enzyme inducers and tumor promoters in vivo) or with 12-O-tetradecanoylphorbol-13-acetate (the classical skin tumor promoter) further increased EGF-stimulated DNA synthesis. The results emphasize the need to adequately substitute zinc in serum-free cultured cells.
Kobusch
A B
AB
Institute of Toxicology, University of Tübingen, Federal Republic of Germany.
Bock
K W
KW
D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002712:Chlorides / Q000494:pharmacology*; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis*; D004347:Drug Interactions; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010634:Phenobarbital / Q000494:pharmacology; D011078:Polychlorinated Biphenyls / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology*; D017967:Zinc Compounds
D002273:Carcinogens; D002712:Chlorides; D003470:Culture Media; D017967:Zinc Compounds; D004815:Epidermal Growth Factor; C016837:zinc chloride; D004247:DNA; D011078:Polychlorinated Biphenyls; D007770:L-Lactate Dehydrogenase; D015032:Zinc; D013755:Tetradecanoylphorbol Acetate; C028451:3,4,3',4'-tetrachlorobiphenyl; D010634:Phenobarbital