title: Preparation and duplex-to-single strand transition of the fully complementary duplex of d(G4).d(C4) in aqueous solution.
abstract: The d(G4) and d(C4) molecules in the single stranded state were synthesized by the phosphotriester method and purified. The full duplex of tetramer d(G4).d(C4) was prepared by expending about a month. The duplex-to-single strand transition was observed by UV-spectroscopy. A standard hypochromic effect was observed, which is different from some experimental results reported previously.
authors.affiliation: National Chemical Laboratory for Industry, Tsukuba Research Center, Ibaraki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Shinoda
authors.forename: T
authors.initials: T
authors.lastname: Wada
authors.forename: S
authors.initials: S
authors.lastname: Ohuchi
authors.forename: T
authors.initials: T
authors.lastname: Hata
mesh_terms: D004277:DNA, Single-Stranded / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D011066:Poly C / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D011068:Poly G / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D011089:Polydeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D012996:Solutions; D014867:Water
chemical_list: D004277:DNA, Single-Stranded; D011089:Polydeoxyribonucleotides; D012996:Solutions; D014867:Water; D011068:Poly G; C023826:poly(dC); C026628:poly(dG); C019530:poly(dC-dG); D011066:Poly C