The detection and characterization of G-proteins in the eyespot of Chlamydomonas reinhardtii.
The presence of G-proteins in the eyespot fraction of Chlamydomonas reinhardtii is shown. This fraction is capable of binding (GTP gamma[35S], possesses the GTPase activity and interacts with antibodies raised against a highly conserved peptide of most G-proteins' alpha-subunit. Cross-reaction with a 24-kDa protein is detected on immunoblots. Using an antiserum prepared from vertebrate beta-subunit peptide, two additional proteins with apparent Mr 21 and 29 kDa could be revealed. The light-dependence of GTPase extraction from eyespot membranes is shown. The results make it possible to suggest the participation of G-proteins in the photosensory transduction chain of Ch. reinhardtii.
Korolkov
S N
SN
Sechenov Institute of Evolutionary Physiology and Biochemistry, USSR Academy of Sciences, Leningrad.
Garnovskaya
M N
MN
Basov
A S
AS
Chunaev
A S
AS
Dumler
I L
IL
D000906:Antibodies; D002696:Chlamydomonas / Q000502:physiology*; D020558:GTP Phosphohydrolases / Q000502:physiology; D019204:GTP-Binding Proteins / Q000302:isolation & purification / Q000502:physiology*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism; D015151:Immunoblotting; D008027:Light; D008970:Molecular Weight; D013462:Sulfur Radioisotopes; D015293:Transducin / Q000502:physiology
D000906:Antibodies; D013462:Sulfur Radioisotopes; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D015293:Transducin