Glycosphingolipid receptors for Pseudomonas aeruginosa.
The binding of Pseudomonas aeruginosa to glycosphingolipids and to buccal and bronchial epithelial cells was analyzed. Three independently expressed specificities were found by bacterial binding to glycosphingolipids separated by thin-layer chromatography. All strains bound gangliotria- and gangliotetrasylceramide. All but one of the strains bound sialic acid-containing glycosphingolipids and lactosylceramide. The latter two specificities could be separated in that the lactosylceramide binding was retained and the sialic acid binding was suppressed when bovine serum albumin was used as a blocking agent in the thin-layer chromatography assay. The attachment to buccal epithelial cells, like the binding to sialylated compounds and lactosylceramide, was abolished by Formalin treatment of the bacteria, suggesting the importance of these specificities for cell adherence. In contrast, the binding to gangliotria- and gangliotetraosylceramide was retained by nonattaching Formalin-treated bacteria.
Baker
N
N
Department of Clinical Immunology, University of Lund, Sweden.
Hansson
G C
GC
Leffler
H
H
Riise
G
G
Svanborg-Edén
C
C
D015703:Antigens, CD; D001422:Bacterial Adhesion / Q000502:physiology*; D002240:Carbohydrate Sequence; D004848:Epithelium / Q000382:microbiology; D006028:Glycosphingolipids / Q000378:metabolism*; D006801:Humans; D007790:Lactosylceramides; D008969:Molecular Sequence Data; D009055:Mouth / Q000382:microbiology; D019158:N-Acetylneuraminic Acid; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D012794:Sialic Acids / Q000378:metabolism
D015703:Antigens, CD; D006028:Glycosphingolipids; D007790:Lactosylceramides; D012794:Sialic Acids; C009744:CDw17 antigen; D019158:N-Acetylneuraminic Acid