title: Evidence for the presence of a phosphatidylinositol anchor on the lipoarabinomannan and lipomannan of Mycobacterium tuberculosis.
abstract: The recent availability (Hunter, S.W., Gaylord, H., and Brennan, P.J. (1986) J. Biol. Chem. 261, 12345-12351) of the well known arabinomannan of Mycobacterium leprae and Mycobacterium tuberculosis as the pure native lipoarabinomannan has resulted in its implication in key aspects of the immunopathogenesis of leprosy and tuberculosis. We had indicated that the lipid moiety of lipoarabinomannan is probably based on a diacylglycerol unit in that glycerol and the two fatty acids, hexadecanoate and 10-methyloctadecanoate, were identified. In addition, lipoarabinomannan was also shown to contain myo-inositol 1-phosphate. Evidence is now presented, based on selective radiolabeling and analysis of various cleavage fragments, that the inositol phosphate exists as both an alkalilable phosphodiester and as part of a phosphatidylinositol "membrane anchor." The mannan of M. tuberculosis was also isolated as the native lipomannan. It also apparently contains a phosphatidylinositol unit but is devoid of the alkali-labile inositol phosphate residues. These lipopolysaccharides are apparently multiglycosylated versions of the well known myocobacterial mannosyl phosphatidylinositols and are prokaryotic versions of the growing list of phosphatidylinositol-anchored macromolecules. Immunogold labeling demonstrates that lipoarabinomannan is a true antigenic capsular or extracellular product of M. tuberculosis. The presence of a phosphatidylinositol residue on lipoarabinomannan may explain its interaction with macrophage membranes and role in mycobacterial pathogenesis.
authors.affiliation: Department of Microbiology, Colorado State University, Fort Collins 80523.
authors.forename: S W
authors.initials: SW
authors.lastname: Hunter
authors.forename: P J
authors.initials: PJ
authors.lastname: Brennan
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002462:Cell Membrane / Q000032:analysis; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D007150:Immunohistochemistry; D007295:Inositol Phosphates / Q000032:analysis; D008070:Lipopolysaccharides / Q000032:analysis*; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000032:analysis*; D010716:Phosphatidylinositols / Q000032:analysis*; D010766:Phosphorylation
chemical_list: D000942:Antigens, Bacterial; D007295:Inositol Phosphates; D008070:Lipopolysaccharides; D010716:Phosphatidylinositols; C050016:lipoarabinomannan; C064351:lipomannan