Human IFN-gamma up-regulates IL-2 receptors in mitogen-activated T lymphocytes.
This study examined the role of human recombinant interferon-gamma (rIFN-gamma) in the expression of interleukin-2 receptors (IL-2R) by human T lymphocytes. rIFN-gamma enhanced total numbers of IL-2R in mitogen-activated but not resting T cells. Scatchard plot analysis indicated that rIFN-gamma increased both high- and low-affinity receptors, with a predominant effect on the latter. Phytohaemagglutinin (PHA)-activated T cells treated with IFN-gamma showed higher IL-2 binding and greater IL-2 internalization and degradation than cells treated with PHA alone. There was a corresponding increase of mitogen-driven proliferative responses, indicating an increase of functional receptors in IFN-treated cultures. IFN-gamma may influence T-cell activation and proliferation by enhancing expression of IL-2R and promoting IL-2 uptake by mitogen-activated lymphocytes.
Rodriguez
M A
MA
Centro Nacional de Enfermedades Reumaticas, Hospital Universitario de Caracas, Venezuela.
De Sanctis
J B
JB
Blasini
A M
AM
Leon-Ponte
M
M
Abadi
I
I
D002478:Cells, Cultured; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D007376:Interleukin-2 / Q000378:metabolism; D008213:Lymphocyte Activation; D010835:Phytohemagglutinins; D015375:Receptors, Interleukin-2 / Q000378:metabolism*; D011994:Recombinant Proteins; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects*
D007376:Interleukin-2; D010835:Phytohemagglutinins; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins; D007371:Interferon-gamma