High efficiency electroporation of Pseudomonas aeruginosa using frozen cell suspensions.
High efficiency transformation of Pseudomonas aeruginosa was achieved using frozen cell suspensions and high voltage electroporation. We have obtained frequencies as high as 5.8 x 10(8) transformants/micrograms of plasmid DNA using PA01 strain OT684 and a buffer of 15% glycerol-1 mM MOPS. The method allows for easy and reproducible production of frozen cell suspensions for rapid transformation of P. aeruginosa.
Farinha
M A
MA
Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada.
Kropinski
A M
AM
D004269:DNA, Bacterial / Q000494:pharmacology; D004563:Electrochemistry; D005615:Freezing; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics*; D014169:Transformation, Bacterial
D004269:DNA, Bacterial