Expression of I-A alpha k and allelic restriction on A alpha/A beta pairing in transgenic mice.
Cell surface expression of class 11 major histocompatibility complex encoded (1a) molecules depends on association of the component alpha and beta chain into a stable heterodimer. Studies on cell lines transfected with MHC class II genes have revealed important limitations on the assembly of haplotype-mismatched A alpha:A beta complex. In order to study alpha:beta chain pairing restrictions in vivo a number of lines of transgenic mice carrying the A alpha k gene were generated. The transgene was studied in the context of H-2b, H-2s, H-2q, H-2u, H-2f and H-2v haplotypes. Initial FACS analysis of spleen and peripheral blood cells showed no A alpha kappa expression. Spleen cells stimulated with LPS and analysed by FACS showed A alpha k expression in three different H-2b strains as well as H-2u, but not in others. These data indicate that A alpha k can associate with A beta b and A alpha k, but not with A beta s, A beta q, A beta f, A beta v.
Martin
J
J
Department of Immunology, Mayo Clinic and Medical School, Rochester, MN 55905.
Wei
B Y
BY
Savarirayan
S
S
David
C S
CS
D000483:Alleles; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D003433:Crosses, Genetic; D015870:Gene Expression; D005802:Genes, MHC Class II; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic
D000949:Histocompatibility Antigens Class II