The effect of four chemical forms of selenium on mammary tumor incidence in BALB/c female mice treated with 7-12-dimethylbenz[a]anthracene.
The study was conducted to determine the effect of four forms of selenium on inhibition of DMBA-induced mammary tumors. BALB/c virgin female mice were fed the AIN-76 diet containing 0.2 or 2.0 ppm Se as selenite, selenate, selenomethionine, or selenocystine prior to and for 6 months post DMBA-treatment. At necropsy, mammary glands were histologically treated for confirmation of adenocarcinomas and the livers were removed for analysis of glutathione-peroxidase (GSHPx) activity and selenium concentrations. Dietary levels or forms of selenium had no effect on body weights. Inorganic selenium fed at 2.0 ppm Se (selenite and selenate) decreased mammary tumor incidence, but organic selenium (selenomethionine and selenocystine) had no effect on mammary tumor incidence. Hepatic GSHPx activity was highest with the 2.0 ppm selenium as selenocystine diet, but hepatic selenium levels were highest with the 2.0 ppm selenium as selenite. This study showed that the dietary form of selenium affects inhibition of mammary tumorigenesis. Furthermore, the study suggested that the pathways for selenium incorporation into GSHPx and for tumor inhibition are different.
Lane
H W
HW
Department of Nutrition and Foods, College of Veterinary Medicine, Auburn University 36849.
Teer
P
P
Dukes
J
J
Johnson
J
J
White
M T
MT
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D003553:Cystine / Q000031:analogs & derivatives / Q000494:pharmacology; D005260:Female; D005979:Glutathione Peroxidase / Q000032:analysis; D008099:Liver / Q000032:analysis; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D016566:Organoselenium Compounds; D064586:Selenic Acid; D020887:Selenious Acid; D012643:Selenium / Q000032:analysis / Q000494:pharmacology*; D018036:Selenium Compounds; D012645:Selenomethionine / Q000494:pharmacology
D016566:Organoselenium Compounds; D018036:Selenium Compounds; C009226:selenocystine; D003553:Cystine; D015127:9,10-Dimethyl-1,2-benzanthracene; D012645:Selenomethionine; D005979:Glutathione Peroxidase; D020887:Selenious Acid; D012643:Selenium; D064586:Selenic Acid