[A chlorotetracycline fluorescent probe--an indicator of calcium ion binding with calcium-binding proteins].
It has been found in in vitro experiments that fluorescence intensity of deionized solution containing a chlorotetracycline fluorescent probe increases insignificantly at the addition of calmodulin of S-100 proteins. Subsequent introduction of Ca2+ into the medium results in the pronounced fluorescence increase depending on Ca2+ concentration. Addition of specific protein blockers--W7 (calmodulin inhibitor) and antibodies to S-100 brought about a decrease of fluorescence. In in vivo experiments on chlorotetracycline-stained neurons of Helix Pomatia ganglia subesophageal complex it has been shown that bringing of antibodies to S-100 and calmodulin significantly decreases the fluorescence intensity of these cells. These data suggest that the chlorotetracycline probe is an indicator of calcium ions binding with calcium-binding proteins both in in vitro and in vivo systems.
Nikitin
V P
VP
Samoĭlov
M O
MO
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002413:Cations, Divalent; D002751:Chlortetracycline / Q000737:chemistry*; D005456:Fluorescent Dyes; D006372:Helix, Snails
D002135:Calcium-Binding Proteins; D002413:Cations, Divalent; D005456:Fluorescent Dyes; D002118:Calcium; D002751:Chlortetracycline
Khlortetratsiklinovyĭ fluorestsentnyĭ zond--indikator sviazyvaniia ionov kal'tsiia kal'tsiĭsviazyvaiushchimi belkami.