Immunoglobulin VH gene expression following hemorrhage.
Hemorrhage has multiple effects on immunologic response, including alteration of B cell repertoires. In limiting dilution studies, decreased absolute frequencies of splenic clonal precursors specific for bacterial antigens were found after blood loss. In order to better define the effects of hemorrhage on B cell function, we examined immunoglobulin VH gene family expression following blood loss using both in situ hybridization and the RNA colony blot technique. No changes in VH gene family utilization were found after hemorrhage. These results demonstrate that the hemorrhage induced alteration in B cell function involves all VH gene families, without modifying distributive frequencies in VH gene family expression.
Abraham
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E
Department of Medicine, UCLA Medical Center 90024.
Andrade
L
L
Freitas
A A
AA
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002999:Clone Cells; D005786:Gene Expression Regulation / Q000276:immunology; D006470:Hemorrhage / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000276:immunology; D013234:Stem Cells / Q000276:immunology; D013997:Time Factors; D014158:Transcription, Genetic
D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D008070:Lipopolysaccharides