title: Yeast RAS2 affects cell viability, mitotic division and transient gene expression in Nicotiana species.
abstract: Overexpression of the budding yeast RAS2 gene in Nicotiana plumbaginifolia cells revealed that RAS2 acted as 'suicide' gene in freshly isolated protoplasts from leaves and blocked cell proliferation in cell suspension-derived protoplasts. Among a series of genes tested (such as npt II, CDC35, PDE2), RAS2 was the only one to block the expression of the cat gene, as measured in a transient gene expression assay. Another ras gene, v-Ha-ras, had similar effects. Furthermore, the RAS2 effect was species-specific and depended on the modulation of hormonal metabolism in the transfected cells, while no differences were noticed between the normal and the activated val19 gene. Transfected plant cells are shown to synthesize a RAS2 protein of the same electrophoretic mobility as the yeast RAS2 product. The results are discussed in the broader context of the evolutionarily conserved ras genes involved in vital cellular functions.
authors.affiliation: Unité de Biologie Moléculaire et de Physiologie Animale, Faculté des Sciences Agronomiques de l'Etat, Gembloux, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Hilson
authors.forename: J
authors.initials: J
authors.lastname: Dewulf
authors.forename: F
authors.initials: F
authors.lastname: Delporte
authors.forename: P
authors.initials: P
authors.lastname: Installé
authors.forename: J M
authors.initials: JM
authors.lastname: Jacquemin
authors.forename: M
authors.initials: M
authors.lastname: Jacobs
authors.forename: I
authors.initials: I
authors.lastname: Negrutiu
mesh_terms: D002454:Cell Differentiation / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics; D015870:Gene Expression; D005800:Genes, Fungal; D011905:Genes, ras; D005818:Genetic Engineering; D008938:Mitosis / Q000235:genetics; D059828:Plant Cells; D010944:Plants / Q000235:genetics* / Q000378:metabolism; D010947:Plants, Toxic; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D014026:Nicotiana / Q000166:cytology / Q000235:genetics; D014170:Transformation, Genetic; D018631:ras Proteins
chemical_list: D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; C489015:RAS2 protein, S cerevisiae; D018631:ras Proteins