The tertiary structure of a bacterial cellulase determined by small-angle X-ray-scattering analysis.
CenA from Cellulomonas fimi is a beta-1,4-endoglucanase that binds tightly to cellulose. X-ray-scattering analyses show that the enzyme is tadpole-shaped: the previously identified catalytic and cellulose-binding domains comprise the head and tail respectively. It appears that this structural and functional organization is common to several cellulases from bacteria and fungi.
Pilz
I
I
Institut für Physikalische Chemie, Universität Graz, Austria.
Schwarz
E
E
Kilburn
D G
DG
Miller
R C
RC
Warren
R A
RA
Gilkes
N R
NR
D000192:Actinomycetales / Q000201:enzymology*; D002480:Cellulase / Q000737:chemistry*; D011487:Protein Conformation; D012542:Scattering, Radiation; D014242:Trichoderma / Q000201:enzymology; D014965:X-Rays
D002480:Cellulase