Two-dimensional electrophoresis of proteins: a simple procedure for casting of narrow-bore, first dimension (isoelectric focusing) polyacrylamide tube gels.
A convenient and rapid method for casting of narrow-bore (1.5-2.5 mm, i.d.) first dimension isoelectric focusing gel tubes is described. The procedure utilizes a peristaltic pump and a 2-inch, 23 gauge blunt needle for dispensing the gel into fixed tubes on a 1.1 degree incline. The method is simple to perform, precludes the formation of air bubbles and little or no manual dexterity is required.
Baumstark
J S
JS
Department of Obstetrics and Gynecology, Creighton University, School of Medicine, Omaha, Nebraska 68131.
Chang
R S
RS
D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D007525:Isoelectric Focusing / Q000295:instrumentation* / Q000379:methods; D011506:Proteins / Q000032:analysis*
D011506:Proteins