Synthesis and characterization of gentiobiose heptaacetate conjugate vaccines that produce endotoxin-neutralizing antibodies.
We have prepared aminoethyl (AE), aminopropyl (AP), and aminopentyl (APT) derivatives of gentiobiose heptaacetate (GH). These spacer compounds (AEGH, APGH, APTGH) have been coupled to succinylated diphtheria toxoid (Suc.DT) to produce conjugate vaccines. These conjugates all bind to the anti-lipid A human monoclonal antibody A6(H4C5) in an ELISA binding assay. Rabbits immunized with the APGH conjugate vaccine in either Freund's complete adjuvant or aluminum hydroxide gel produced antibody levels of 5120 and 3600 ELISA units, respectively, compared to an antibody level of less than 20 ELISA units for the prebleed sera. Sera from mice immunized with either the aminopropyl or the aminopentyl conjugate had antibody levels of 5120 and 2560 ELISA antibody units, respectively. These antibodies neutralized endotoxin in a Limulus lysate neutralization assay. Protection against the local Shwartzman reaction was demonstrated (p less than 0.05) in eight out of nine rabbits immunized with the Suc-DT-APGH conjugate vaccine compared to three out of 10 rabbits immunized with the carrier protein Suc-DT. Passive transfer experiments demonstrated that four out of five rabbits receiving immune serum were protected from Shwartzman reaction compared to one out of five rabbits receiving normal serum (p less than 0.1). These results indicated that epitopes contained in gentiobiose heptaacetate when properly presented as conjugate vaccines were capable of inducing neutralizing antibodies against endotoxin.
Bhattacharjee
A K
AK
Department of Bacterial Diseases, Walter Reed Army Institute of Research, Washington, DC 20307-5100.
Sadoff
J C
JC
Collins
H
H
Cross
A S
AS
Khalil
A H
AH
Bieber
M M
MM
Wright
C
C
Teng
N N
NN
D000588:Amines / Q000138:chemical synthesis* / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000097:blood; D000911:Antibodies, Monoclonal / Q000276:immunology; D000917:Antibody Formation; D015153:Blotting, Western; D004168:Diphtheria Toxoid / Q000276:immunology*; D004731:Endotoxins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005960:Glucosides / Q000138:chemical synthesis* / Q000276:immunology; D007114:Immunization; D008050:Lipid A / Q000276:immunology; D051379:Mice; D009500:Neutralization Tests; D011437:Propylamines / Q000138:chemical synthesis* / Q000276:immunology; D011817:Rabbits; D012790:Shwartzman Phenomenon / Q000276:immunology / Q000517:prevention & control; D013386:Succinates / Q000276:immunology*; D014612:Vaccines / Q000276:immunology*
D000588:Amines; D000906:Antibodies; D000911:Antibodies, Monoclonal; D004168:Diphtheria Toxoid; D004731:Endotoxins; D005960:Glucosides; D008050:Lipid A; D011437:Propylamines; D013386:Succinates; D014612:Vaccines; C069570:succinylated diphtheria toxoid; C069572:5-aminopentyl-2,3,4,2',3',4',6'-hepta-O-acetylgentiobioside; C069571:3-aminopropyl 2,3,4,2',3',4',6'-hepta-O-acetylgentiobioside