title: Identification and design of binding determinants in proteins.
abstract: Mutagenic analysis are described for probing and designing binding determinants of two different proteins. The first protein is an enzyme, subtilisin, for which structural models were available for the enzyme-substrate complex. The second protein is human growth hormone for which a structural model of its complex with receptor was not available. In both cases, it was possible to identify and engineer binding determinants that lead to large changes in affinity.
authors.affiliation: Department of Biomolecular Chemistry, Genentech, Inc., South San Francisco, CA 94080.
authors.forename: J A
authors.initials: JA
authors.lastname: Wells
authors.forename: B C
authors.initials: BC
authors.lastname: Cunningham
mesh_terms: D015195:Drug Design; D013006:Growth Hormone / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006801:Humans; D011485:Protein Binding; D015202:Protein Engineering; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011986:Receptors, Somatotropin / Q000378:metabolism
chemical_list: D011506:Proteins; D011986:Receptors, Somatotropin; D013006:Growth Hormone