title: G protein mRNA expression in immunohistochemically identified dopaminergic and noradrenergic neurons in the rat brain.
abstract: A family of guanine nucleotide binding proteins (G proteins) is involved in the transduction of information from receptors on the cell surface into cellular responses. Two G proteins, Gi and Gs, were initially defined by their inhibitory or stimulatory actions on adenylyl cyclase, respectively. In addition, brain contains high levels of another G protein, Go. cDNAs for the alpha subunits for these G proteins have been cloned and sequenced. This allowed us to examine the distributions of the mRNAs for the alpha subunits for Gi, Go and Gs in the rat brain using in situ hybridization with radio-labelled, synthetic oligonucleotide probes. Various regions known to contain catecholamine cell groups displayed high levels of G protein mRNA. There is good physiological evidence supporting a role for G proteins in signal transduction in dopaminergic and noradrenergic neurons. Therefore, further experiments were undertaken using in situ hybridization combined with immunohistochemistry to examine G proteins expression in identified catecholamine neurons. The results indicate that the dopaminergic neurons of the substantia nigra and the noradrenergic neurons of the locus ceruleus express the mRNA for the alpha subunits of all three of these G proteins. These data provide evidence for the coexpression of multiple G proteins within identified catecholamine neurons in the brain.
authors.affiliation: Department of Psychiatry, University of British Columbia, Vancouver, Canada.
authors.forename: S R
authors.initials: SR
authors.lastname: Vincent
authors.forename: B T
authors.initials: BT
authors.lastname: Hope
authors.forename: S L
authors.initials: SL
authors.lastname: Drinnan
authors.forename: P B
authors.initials: PB
authors.lastname: Reiner
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D004298:Dopamine / Q000502:physiology*; D019204:GTP-Binding Proteins / Q000235:genetics*; D007124:Immunoenzyme Techniques; D008297:Male; D009474:Neurons / Q000378:metabolism*; D009638:Norepinephrine / Q000502:physiology*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D012333:RNA, Messenger; D019204:GTP-Binding Proteins; D004298:Dopamine; D009638:Norepinephrine