title: Chlorobutanol, a preservative of desmopressin, inhibits human platelet aggregation and release in vitro.
abstract: Therapeutic preparations of desmopressin for parenteral use contain the preservative chlorobutanol (5 mg/ml). We show here that chlorobutanol is a potent inhibitor of platelet aggregation and release. It exhibited a significant inhibitory activity toward several aggregation inducers in a concentration- and time-dependent manner. Thromboxane B2 formation, ATP release, and elevation of cytosolic free calcium caused by collagen, ADP, epinephrine, arachidonic acid and thrombin respectively were markedly inhibited by chlorobutanol. Chlorobutanol had no effect on elastase-treated platelets and its antiplatelet effect could be reversed. It is concluded that the antiplatelet effect of chlorobutanol is mainly due to its inhibition on the arachidonic acid pathway but it is unlikely to have a nonspecific toxic effect. This antiplatelet effect of chlorobutanol suggests that desmopressin, when administered for improving hemostasis, should not contain chlorobutanol as a preservative.
authors.affiliation: Department of Internal Medicine, Veterans General Hospital Taipei, Taiwan, R.O.C.
authors.forename: S L
authors.initials: SL
authors.lastname: Chen
authors.forename: W C
authors.initials: WC
authors.lastname: Yang
authors.forename: T P
authors.initials: TP
authors.lastname: Huang
authors.forename: S A
authors.initials: SA
authors.lastname: Wann
authors.forename: C M
authors.initials: CM
authors.lastname: Teng
mesh_terms: D000255:Adenosine Triphosphate / Q000097:blood; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000097:blood; D002724:Chlorobutanol / Q000494:pharmacology*; D003894:Deamino Arginine Vasopressin / Q000008:administration & dosage; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D010196:Pancreatic Elastase; D010975:Platelet Aggregation Inhibitors; D011310:Preservatives, Pharmaceutical / Q000494:pharmacology*; D013929:Thromboxane B2 / Q000096:biosynthesis
chemical_list: D010975:Platelet Aggregation Inhibitors; D011310:Preservatives, Pharmaceutical; D013929:Thromboxane B2; D000255:Adenosine Triphosphate; D010196:Pancreatic Elastase; D003894:Deamino Arginine Vasopressin; D002724:Chlorobutanol; D002118:Calcium