title: Improved method for measuring the catalytic activity of tryptophan synthase alpha subunit in cell extracts.
abstract: An improved method has been developed for measuring the catalytic activity of tryptophan synthase alpha-subunit in cell extracts using the indole-3-glycerol phosphate (InGP)----tryptophan reaction. The method involves the chemical and enzymatic synthesis of the substrate InGP immediately before use and avoids the preparation of salt-free hydroxylamine. The method is more convenient, safer and more reliable than the traditional method employing the InGP----indole reaction.
authors.affiliation: Department of Biochemistry, University of Southern California, School of Medicine 90033.
authors.forename: R D
authors.initials: RD
authors.lastname: Mosteller
mesh_terms: D004926:Escherichia coli / Q000201:enzymology; D005994:Glycerophosphates; D007211:Indoles; D008722:Methods; D011487:Protein Conformation; D013053:Spectrophotometry; D013379:Substrate Specificity; D014367:Tryptophan Synthase / Q000032:analysis* / Q000737:chemistry
chemical_list: D005994:Glycerophosphates; D007211:Indoles; C008117:indoleglycerol phosphate; D014367:Tryptophan Synthase