Long-wave ultraviolet light induces phospholipase activation in cultured human epidermal keratinocytes.
Long wave ultraviolet radiation (UVA) has been shown to play an important role in the overall response of skin to solar radiation, including sunburn, tanning, premature aging, and non-melanoma skin cancer. UVA induction of inflammation in human skin is thought to be mediated by membrane lipid derived products. In order to investigate the mechanism of this response we examined the effect of UVA on phospholipid metabolism of human epidermal keratinocytes in culture. Keratinocytes were grown in serum free low calcium medium. The cells were prelabeled with [3H] arachidonic acid or [3H] choline and irradiated with UVA (Honle 2002-Hg vapor lamp). Identification and quantitation of specific membrane phospholipid-derived components was achieved using high-performance liquid chromatography, paper chromatography, and radioimmunoassay. UVA resulted in a linear dose dependent release of [3H] arachidonic acid into medium between 1 and 20 joule/cm2. This response was inhibited in an oxygen-reduced environment. The radiolabel released was predominantly free arachidonate and cyclooxygenase metabolites. Cyclooxygenase metabolites prostaglandin E2 and prostacyclin derivative, 6-keto-prostaglandin F1a, were stimulated following UVA irradiation, but the lipoxygenase metabolite, leukotriene B was not detected. Maximal release was measured immediately after irradiation and changed little over 24 h post-irradiation. UVA stimulated an increase of [3H] choline metabolites glycerophosphorylcholine and phosphorylcholine in media extracts suggesting UVA activation of phospholipase C and phospholipase A2 or diacylglyceride lipase.
Hanson
D
D
Department of Dermatology, Columbia University, New York, New York 10032.
DeLeo
V
V
D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002478:Cells, Cultured; D002794:Choline / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D004789:Enzyme Activation; D006801:Humans; D015603:Keratinocytes / Q000201:enzymology / Q000528:radiation effects*; D007700:Kinetics; D010740:Phospholipases / Q000378:metabolism / Q000528:radiation effects*; D011453:Prostaglandins / Q000096:biosynthesis; D014466:Ultraviolet Rays
D001095:Arachidonic Acids; D011453:Prostaglandins; D016718:Arachidonic Acid; D010740:Phospholipases; D002794:Choline