Inhibition of platelet activation by the nitrate Sin-1: studies with human aequorin-loaded platelets.
The effects of the nitrovasodilator Sin-1, which is thought to work through cyclic-GMP, were studied using human, aequorin-loaded, washed platelets. Changes in light transmission (shape change, aggregation), and in intracellular calcium were simultaneously recorded. Evidence was obtained for an inhibitory effect distal to calcium changes, in a similar way than a prostacyclin analogue, Iloprost. By contrast the response to an activator of protein kinase C was unaffected. There was a partial, parallel decrease in calcium changes and aggregation induced with thrombin, and a total inhibition of the responses to ADP (linked to a calcium influx).
Dutrillaux
F
F
Laboratoire Central d'Hématologie, Hôpital de l'Hôtel-Dieu, Paris, France.
Potevin
F
F
Lecompte
T
T
D000331:Aequorin; D006801:Humans; D066298:In Vitro Techniques; D008981:Molsidomine / Q000031:analogs & derivatives* / Q000494:pharmacology; D010975:Platelet Aggregation Inhibitors
D010975:Platelet Aggregation Inhibitors; D000331:Aequorin; C002385:linsidomine; D008981:Molsidomine