Cooperativity between platelet-activating factor and collagen in aggregation of bovine platelets III.
In cooperative aggregation of bovine platelets induced by simultaneous addition of PAF and collagen at subthreshold concentrations the following observations were made. (i) Formation of phosphatidic acid and arachidonic acid metabolites, which characterize PAF and collagen alone-induced aggregation, respectively, was observed very obviously. (ii) A thromboxane antagonist did not completely block the cooperative aggregation. The extent of residual aggregation activity was dependent on concentration of collagen used in the simultaneous administration with PAF. These results suggest that both signal transduction pathways activated by PAF and collagen alone at high concentrations are attained by simultaneous addition of both agonists at subthreshold concentrations through unknown mechanisms.
Kojima
S
S
Department of Biological Sciences, Tokyo Institute of Technology, Japan.
Sekiya
F
F
Inada
Y
Y
Sato
F
F
Tsukada
T
T
Saito
Y
Y
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000096:biosynthesis; D002417:Cattle; D003094:Collagen / Q000494:pharmacology*; D004357:Drug Synergism; D010743:Phospholipids / Q000096:biosynthesis; D010972:Platelet Activating Factor / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*
D001095:Arachidonic Acids; D010743:Phospholipids; D010972:Platelet Activating Factor; D016718:Arachidonic Acid; D003094:Collagen