[Screening of alpha-amino acid ester hydrolase producing strain and synthesis of cephalexin by Pseudomonas aeruginosa].
From ten genera and 146 bacterial strains, 22 strains producing alpha-amino acid ester hydrolase were selected. Among them, AS 1.586 and 41-2 were the best. The optimal conditions for synthesis of cephalexin by pseudomonas aeruginosa 1.204 were investigated. The optimal pH and temperature for enzymatic synthesis reaction was pH 6.8 and 25 degrees C, respectively. By using 1% 7-ADCA, 3% PGME and 4% biomass, about 70% of 7-ADCA was converted to cephalexin under the mentioned conditions.
Wang
M
M
Institute of Microbiology, Academia Sinica, Beijiag.
Wang
Z
Z
Yue
H
H
Han
W
W
Jiao
Q
Q
D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002506:Cephalexin / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D013696:Temperature
D002265:Carboxylic Ester Hydrolases; C058058:alpha-amino acid esterase; D002506:Cephalexin