Age-related changes in Schwann cells of the internal branch of the rat superior laryngeal nerve.
While previous studies have examined the role of neurons in age-related changes of laryngeal sensory nerve function, investigators have not focused on the significant role of Schwann cells in the aging process. This investigation used an electron microscopic morphometric technique to systematically study Schwann cells of the internal branch of the superior laryngeal nerve of three Wistar rat age groups: young = 3 to 5 months; old = 25 months; very old = 29 to 31 months. In Schwann cells of myelinated axons, a substantial and significant reduction (p = 0.0127) in mitochondrial volume fraction was found between the young and old groups, and also between the young and very old groups (young = 0.0034, old = 0.00175, very old = 0.0019). The volume fraction of compact myelin also showed a trend (p = 0.0683) toward decreasing with age. The volume fractions of cytoplasm, nuclei, and lipofuscin granules showed no significant age-related changes (lipofuscin in myelinating Schwann cells: young = 0.0025, old = 0.0020, very old = 0.0051; lipofuscin in non-myelinating Schwann cells: young = 0.012, old = 0.0061, very old = 0.0051). Our observations on mitochondria lead to two possible conclusions regarding energy availability in aged Schwann cells. One possibility is that aged cells have a decreased energy requirement, which is reflected in the decreased mitochondrial densities in old and very old specimens. The other possibility is the reduced mitochondrial volume fractions are independent of cellular energy requirements and are indicative of some aging or pathologic process.(ABSTRACT TRUNCATED AT 250 WORDS)
Choo
D
D
State University of New York Health Science Center, Syracuse 13210.
Malmgren
L T
LT
Rosenberg
S I
SI
D000375:Aging / Q000473:pathology*; D000818:Animals; D001369:Axons / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D005260:Female; D007823:Laryngeal Nerves / Q000473:pathology*; D008062:Lipofuscin / Q000032:analysis; D008928:Mitochondria / Q000648:ultrastructure; D009185:Myelin Proteins / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D012583:Schwann Cells / Q000648:ultrastructure*
D008062:Lipofuscin; D009185:Myelin Proteins