Development of a DNA-adsorbent for the specific removal of anti-DNA autoantibodies in systemic lupus erythematosus (SLE).
Autoantibodies against DNA are of primary importance for the diagnosis and pathogenesis of systemic lupus erythematousus (SLE). The level of anti-DNA antibodies correlates well with the disease activity and renal involvement. In such patients the removal of anti-DNA antibodies from plasma may lead to a clinical improvement. For this reason an adsorbent was made by covalent coupling of calf thymus DNA to a solid support based on ethylene dimethacrylate cross-linked hydroxethyl methacrylate. Up to 2.5 mg of DNA were immobolized to 1 ml of the support activated chemically by aminosilane and glutaraldehyde. The incubation of 40 ml of SLE plasma with 1 ml of the adsorbent resulted in a 50% decline in anti-DNA activity. There was no release of immobilized P-32-DNA into the plasma. Biocompatibility, sterilisation, and reapplication (without loss of binding capacity) of the adsorbent could be demonstrated. We concluded that the adsorbent may be suitable for treatment.
Hiepe
F
F
Dept. Int. Med., Humboldt University, Berlin, GDR.
Wolbart
K
K
Schossler
W
W
Speer
A
A
Montag
T
T
Mielke
F
F
Apostoloff
E
E
D001323:Autoantibodies / Q000302:isolation & purification*; D001672:Biocompatible Materials; D003167:Complement Activation / Q000276:immunology; D003165:Complement System Proteins / Q000032:analysis*; D004247:DNA / Q000276:immunology*; D006801:Humans; D007163:Immunosorbent Techniques; D007164:Immunosorbents; D008180:Lupus Erythematosus, Systemic / Q000628:therapy*; D008689:Methacrylates; D010951:Plasma Exchange; D012821:Silanes; D013950:Thymus Gland
D001323:Autoantibodies; D001672:Biocompatible Materials; D007164:Immunosorbents; D008689:Methacrylates; D012821:Silanes; C005044:hydroxyethyl methacrylate; C004919:ethylene dimethacrylate; D003165:Complement System Proteins; D004247:DNA