title: Effect of chronic ingestion of a fermented dairy product containing Lactobacillus acidophilus and Bifidobacterium bifidum on metabolic activities of the colonic flora in humans.
abstract: Nine healthy volunteers were studied before, during, and after ingesting a fermented dairy product containing Lactobacillus acidophilus, Bifidobacterium bifidum, and mesophilic cultures (Streptococcus lactis and S cremoris) for 3 wk. Hydrogen and methane productions and fecal beta-galactosidase and beta-glucosidase activities were measured as indicators of fermentation capacity of the colonic flora. Fecal concentrations of nitroreductase, azoreductase, and beta-glucuronidase, which may be implicated in colonic carcinogenesis, were also assessed. Hydrogen and methane productions, fecal beta-galactosidase, beta-glucuronidase, and azoreductase activities did not change over three 3-wk periods whereas fecal beta-glucosidase activity increased (42 +/- 6, 91 +/- 12, and 40 +/- 6 IU/g N, P less than 0.01) and nitroreductase decreased (0.87 +/- 0.13, 0.54 +/- 0.11, and 0.57 +/- 0.08 IU/g N, P less than 0.05).
authors.affiliation: INSERM U290 Fonctions Intestinales, Métabolisme et Nutrition, Hôpital Saint-Lazare, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Marteau
authors.forename: P
authors.initials: P
authors.lastname: Pochart
authors.forename: B
authors.initials: B
authors.lastname: Flourié
authors.forename: P
authors.initials: P
authors.lastname: Pellier
authors.forename: L
authors.initials: L
authors.lastname: Santos
authors.forename: J F
authors.initials: JF
authors.lastname: Desjeux
authors.forename: J C
authors.initials: JC
authors.lastname: Rambaud
mesh_terms: D000328:Adult; D001644:Bifidobacterium / Q000201:enzymology / Q000502:physiology*; D003106:Colon / Q000382:microbiology*; D003611:Dairy Products; D005243:Feces / Q000201:enzymology; D005260:Female; D005285:Fermentation; D005966:Glucuronidase / Q000378:metabolism; D006801:Humans; D006859:Hydrogen; D007779:Lactobacillus acidophilus / Q000502:physiology*; D008297:Male; D008697:Methane; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D009601:Nitroreductases; D012119:Respiration; D013997:Time Factors; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D006859:Hydrogen; D009247:NADH, NADPH Oxidoreductases; D009601:Nitroreductases; C021106:azoreductase; D001617:beta-Glucosidase; D005966:Glucuronidase; D008697:Methane