Polymer-metal complexes of protein antigens--new highly effective immunogens.
The mechanism of interaction of the copolymers N-vinylpyrrolidone-acrylic acid and N-vinylpyrrolidone-maleic anhydride with bovine serum albumin, influenza virus total surface antigen (haemagglutinin and neuraminidase), and the BCG protein fraction in the presence of divalent copper ions was investigated. Novel water-soluble triple polymer-metal complexes of the above protein antigens were formed. These complexes showed high immunogenicity and conferred high levels of immunological protection. Study of the replication of pathogenic influenza A virus in animal lungs showed that, in mice immunised with the triple complex containing surface glycoprotein influenza virus A antigens, reproduction of the homologous virus was sharply inhibited, and immunisation of B mice, exhibiting pronounced T-cell deficiency, with complexes containing the BCG protein fraction ensured development of a high level of protection with respect to BCG infection.
Mustafaev
M I
MI
Institute of Immunology, Ministry of Health of the USSR, Moscow.
Norimov
A Sh
ASh
D000180:Acrylic Resins; D000276:Adjuvants, Immunologic; D000818:Animals; D000941:Antigens; D000956:Antigens, Viral; D001426:Bacterial Proteins / Q000276:immunology; D003300:Copper; D005260:Female; D009980:Influenza A virus / Q000276:immunology; D008670:Metals; D051379:Mice; D008815:Mice, Inbred Strains; D009163:Mycobacterium bovis / Q000276:immunology; D011108:Polymers; D011205:Povidone / Q000031:analogs & derivatives; D011506:Proteins / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology / Q000302:isolation & purification*
D000180:Acrylic Resins; D000276:Adjuvants, Immunologic; D000941:Antigens; D000956:Antigens, Viral; D001426:Bacterial Proteins; D008670:Metals; D011108:Polymers; D011506:Proteins; D014614:Vaccines, Synthetic; C043348:acrylic acid-N-vinylpyrrolidone copolymer; D003300:Copper; D011205:Povidone