title: Ultrastructural immunochemistry of J chains in chickens.
abstract: Using ultrastructural immunochemistry, we have determined subcellular localization of J chain molecules in chicken splenic cells. The majority of J chain positive cells (JPC) were lymphoblast-like cells. The data show that J chains predominantly are localized in cytoplasm with a considerable amount distributed on the cell surface. In the cytoplasm, J chains were diffusely expressed. Furthermore, these J chain molecules were clearly seen as cluster type. In addition to the J chain localization of subcellular organelles as described above, J chains were partly found on perinuclear spaces. As J chains are key protein in B cell differentiation into immunoglobulin (Ig) producing cells, these findings might help for studying regulation of B cell differentiation in addition to revealing the molecular assembly of polymeric Ig.
authors.affiliation: Department of Bacteriology, Saitama Medical School, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Moriya
authors.forename: Y
authors.initials: Y
authors.lastname: Ichikawa
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000276:immunology / Q000648:ultrastructure; D002645:Chickens; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007144:Immunoglobulin J-Chains / Q000032:analysis*; D008214:Lymphocytes / Q000276:immunology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D013154:Spleen / Q000276:immunology* / Q000648:ultrastructure
chemical_list: D007106:Immune Sera; D007144:Immunoglobulin J-Chains