Pretreatment of human vascular smooth muscle cells with interleukin-1 enhances interleukin-6 production and cell proliferation (action of IL-1 on vascular smooth muscle cells).
Systemic vasculitis is an inflammatory disorder of blood vessels characterized by a perivascular mononuclear cell infiltration around the vessel and fibrinoid necrosis within vessel walls. Interleukin-1 (IL-1) is a multipotent inflammatory mediator and affects several properties of vascular cells. To determine whether IL-1 could contribute to the pathogenesis of vascular diseases, we examined the effect of IL-1 on B cell stimulatory factor-2/interleukin-6 (IL-6) production by cultured human vascular smooth muscle cells (SMC) and the proliferation of these cells. Supernatants of SMC stimulated IgM synthesis of human B cell line. SKW6-CL4 cells. This activity was increased (1.7 to 2.6-fold) when SMC were pretreated with IL-1 or calcium ionophore A23187 for 48 h, and was completely blocked by rabbit anti-human IL-6 antibodies. These IL-6 activities of the SMC supernatants were also assessed by using an IL-6 dependent murine hybridoma cell line. MH-60. BSF-2. In addition, we observed that pretreatment of SMC with IL-1 for 48 h stimulated growth of SMC during the 96 h incubations, as assessed by cell number (p less than 0.05). These results suggest that IL-1 may contribute to the pathogenesis of inflammatory and immunological vasculitis by the augmentation of IL-6 release and growth of SMC.
Norioka
K
K
First Department of Medicine, National Defense Medical College, Tokorozawa, Japan.
Hara
M
M
Harigai
M
M
Kitani
A
A
Hirose
T
T
Hirose
W
W
Suzuki
K
K
Kawakami
M
M
Kawagoe
M
M
Nakamura
H
H
D000001:Calcimycin / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D007075:Immunoglobulin M / Q000378:metabolism; D007375:Interleukin-1 / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D011994:Recombinant Proteins / Q000494:pharmacology; D014469:Umbilical Arteries; D014657:Vasculitis / Q000276:immunology
D007075:Immunoglobulin M; D007375:Interleukin-1; D015850:Interleukin-6; D011994:Recombinant Proteins; D000001:Calcimycin