title: [1st isolation of strains of Staphylococcus aureus producing toxic shock syndrome toxin 1 in food handlers in Argentina].
abstract: Thirty nine milk handlers from a factory of dairy products in the Province of Buenos Aires were examined for their nasal carriage of S. aureus strains capable of producing toxic-shock-syndrome toxin-1 (TSST-1). In addition, chance samples of handled foods, crude milk and milky fermented derivates (MFD) were studied. Strain isolation was made on Mannitol Salt Agar and on Baird-Parker Agar. Typical colonies were identified by their biochemical properties. Cultures that were found to be S. aureus were selected for analysis of the TSST-1 production. Eight milk handlers (20.5%) were carriers of S. aureus strains. Seven isolates (87.5%) were classified as biotype A (human ecovar) and 1(12.5%) was classified as biotype B (swine and poultry ecovar). Three out of 8 S. aureus biotype A isolates (37.5%), produced TSST-1. Taking into account the number of milk food handlers sampled (39), the carried rate of toxigenic strains was 7.6%. Three S. aureus strains were isolated from crude milk; 1(33.3%) was classified as biotype B and 2(66.6%) as biotype C (cattle and sheep ecovar). Thirteen S. aureus strains were isolated from MDF; 5(38.0%) were classified as biotype A, 1(7.7%) as belonging to biotype B and 7(53.8%) as belonging to biotype C. None of them had the ability to produce TSST-1.
authors.affiliation: Cátedra de Bacteriología II, Facultad de Química, Bioquímica y Farmacia, San Luis, Argentina.
authors.forename: O N
authors.initials: ON
authors.lastname: Puig de Centorbi
authors.forename: O C
authors.initials: OC
authors.lastname: López
authors.forename: L E
authors.initials: LE
authors.lastname: Alcaraz
authors.forename: A M
authors.initials: AM
authors.lastname: Abdón de Cuadrado
mesh_terms: D000818:Animals; D001118:Argentina / Q000453:epidemiology; D001427:Bacterial Toxins; D002353:Carrier State / Q000453:epidemiology*; D003611:Dairy Products; D004768:Enterotoxins / Q000302:isolation & purification*; D005506:Food Contamination; D005511:Food Handling; D005516:Food Microbiology; D006801:Humans; D008892:Milk; D009296:Nasal Cavity / Q000382:microbiology; D013203:Staphylococcal Infections / Q000635:transmission*; D013211:Staphylococcus aureus / Q000302:isolation & purification* / Q000378:metabolism; D018089:Superantigens
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal
vernacular_title: Primer aislamiento de cepas de Staphylococcus aureus productores de toxina del síndrome de shock tóxico-1 en manipuladores de alimentos en Argentina.