Intramural pericyte degeneration in early diabetic retinopathy study in vitro.
An experimental model of diseased pericytes was established by using cultured bovine retinal capillary pericytes in high--glucose medium. The high glucose stimulated polyol pathway, reduced cellular myo-inositol content and disturbed inositol phospholipid metabolism which resulted in a decrease in inositol trisphosphate (IP3) level. The correlation of suppressed IP3 levels with reduced DNA synthesis was evident. These findings suggested the biochemical mechanism by which retinal pericytes degenerate in high glucose. To supplement myo--inositol and/or an aldose reductase inhibitor to the high--glucose medium largely reversed the suppressed IP3 level and the decreased DNA synthesis. Therefore, these two manipulations may be considered as in vitro therapy to treat sick pericytes induced by high glucose.
Li
W Y
WY
Department of Ophthalmology, Peking Union Medical College Hospital, Beijing.
Zhou
Q
Q
Tang
L
L
Qin
M
M
Hu
T S
TS
D000818:Animals; D001160:Arterioles / Q000473:pathology; D002196:Capillaries / Q000473:pathology; D002417:Cattle; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D003930:Diabetic Retinopathy / Q000378:metabolism / Q000473:pathology*; D005947:Glucose / Q000494:pharmacology*; D007294:Inositol / Q000378:metabolism*; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism*; D012161:Retinal Artery / Q000473:pathology*
D010716:Phosphatidylinositols; D007294:Inositol; D015544:Inositol 1,4,5-Trisphosphate; D004247:DNA; D005947:Glucose