title: Total chemical assembly of genes.
abstract: An artificial gene comprising 183 base pairs has been assembled by template-directed condensation of 35- to 53-membered oligodeoxyribonucleotides with cyanogen bromide as a condensing agent. The reaction is complete within several minutes at 0 degree C in buffer. The resulting gene was cloned into M13mp11 and sequenced using the Sanger sequencing procedure.
authors.affiliation: Chemistry Department, Moscow State University, USSR.
authors.forename: Z A
authors.initials: ZA
authors.lastname: Shabarova
authors.forename: N I
authors.initials: NI
authors.lastname: Sokolova
authors.forename: I N
authors.initials: IN
authors.lastname: Merenkova
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003488:Cyanogen Bromide; D004247:DNA; D005813:Genes, Synthetic; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D015183:Restriction Mapping; D013698:Templates, Genetic
chemical_list: D009838:Oligodeoxyribonucleotides; D004247:DNA; D003488:Cyanogen Bromide