title: [Detection of asymptomatic carriers of hydatid cysts: specificity increase of the immunoenzyme assay].
abstract: An enzymoimmunoassay (EIE) as a screening test to select potential asymptomatic cyst carriers among the general population of areas under risk is being used in programs for the control of hydatic diseases caused by Echinococcus granulosus in Argentina. The experience obtained up to date, applying this assay in population surveys, indicates that depending on the prevalence in the area 10% to 30% of the individuals selected did not show images compatible with hydatic cysts. The purpose of the present study was to improve the specificity of the test. To this purpose, the influence of the modification of the antigenic availability and the effect of the absorption from the serum samples of antibodies anti-normal ovine sera and anti-phosphorylcholine was evaluated. One hundred and fourteen non hydatic sera selected because of their high cross reactivity in EIE using the whole hydatid antigen (WHA) and 118 hydatid sera, were studied with four fractions of ovine hydatid cyst fluid. The EIE employing the S2B antigenic fraction with previous absorption of the sera (EIE-S2B/A) was the system that discriminated better hydatid sera from non hydatid sera with high levels of cross reactivity. The replacement of the EIE employing WHA by the EIE-S2B/A system, for the active search of asymptomatic cyst carriers in field conditions, is proposed. The four antigenic fractions were analyzed by double diffusion and SDS-PAGE. The S2B fraction revealed a high content of parasitic components of less than 30 Kd which probably includes antigen B and subunits or fragments of antigen 5.
authors.affiliation: Centro Panamericano de Zoonosis, Programa de Salud Pública Veterinaria, OPS/OMS, Buenos Aires, Argentina.
authors.forename: E A
authors.initials: EA
authors.lastname: Coltorti
authors.forename: E
authors.initials: E
authors.lastname: Fernández
authors.forename: E R
authors.initials: ER
authors.lastname: Marguet
authors.forename: J D
authors.initials: JD
authors.lastname: Scozzina
authors.forename: E A
authors.initials: EA
authors.lastname: Guarnera
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000032:analysis; D002353:Carrier State; D004443:Echinococcosis / Q000175:diagnosis* / Q000517:prevention & control; D004446:Echinococcus / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007124:Immunoenzyme Techniques
chemical_list: D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth
vernacular_title: Detección de portadores asintomáticos de quistes hidatídicos: aumento de la especificidad del ensayo inmunoenzimático.