title: Low-energy collision-induced dissociation of protonated polyamino alcohol derivatives of peptides and N-terminal blocked peptides.
abstract: This investigation reports the low-energy collision-induced dissociation of the protonated molecules of polyamino alcohols, formed by chemical reduction of synthetic peptides and N-terminal blocked peptides, in order to evaluate its potential for peptide sequence determination. The --CH2--NH-- cleavage with charge retention on the N-terminus was prominent, and the entire sequence of ions produced in this manner was observed. Some of the sequence ions arising from --CH2--NH-- cleavage accompanied by migration of two hydrogens, and --NH--C alpha H-- cleavage with charge retention on the C-terminus, also occurred prominently. In addition, a great number of internal fragment ions were produced in relatively high abundance; these provided supplementary sequence information and were, in some cases, critical in determining the sequence. The usefulness of this method was exemplified by its application to the sequence determination of bacterial lipopeptides.
authors.affiliation: Analytical Research Laboratories, Fujisawa Pharmaceutical Co. Ltd, Osaka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Fujioka
authors.forename: S
authors.initials: S
authors.lastname: Koda
authors.forename: T
authors.initials: T
authors.lastname: Yasuda
mesh_terms: D008074:Lipoproteins / Q000737:chemistry; D009615:Nocardia / Q000032:analysis; D010455:Peptides / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods
chemical_list: D008074:Lipoproteins; D010455:Peptides