title: [Enzyme immunoassays for the quantification of human gamma-gamma-enolase (NSE)].
abstract: A direct two-site binding assay on the basis of antibodies from sheep for the quantification of human gamma-gamma enolase is described. The antibody was produced by immunization with human NSE coupled to horse spleen ferritin. The assay shows two feature: a decreased reactivity with NSE from rat and NSE from human serum in spite of 100% recovery of purified human brain NSE. The sheep antibody seems to react with epitopes less accessible on the rat NSE and on the NSE of human serum. The assay is characterized by gamma-gamma enolase specificity, a high sensitivity (2 pg) and a precision of CV = 3-7%.
authors.affiliation: Institut für Pathologische und Klinische Biochemie, Bereichs Medizin (Charité) der Humboldt-Universität zu Berlin.
authors.forename: J
authors.initials: J
authors.lastname: Gross
authors.forename: J
authors.initials: J
authors.lastname: Zinsmeyer
authors.forename: R
authors.initials: R
authors.lastname: Pohle
authors.forename: R
authors.initials: R
authors.lastname: Moller
authors.forename: H
authors.initials: H
authors.lastname: Schmechta
authors.forename: B
authors.initials: B
authors.lastname: Porstmann
authors.forename: T
authors.initials: T
authors.lastname: Porstmann
authors.forename: B
authors.initials: B
authors.lastname: Brux
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D001921:Brain / Q000201:enzymology*; D006801:Humans; D007124:Immunoenzyme Techniques; D010751:Phosphopyruvate Hydratase / Q000032:analysis* / Q000276:immunology; D011237:Predictive Value of Tests; D051381:Rats; D012756:Sheep; D001616:beta-Galactosidase
chemical_list: D000906:Antibodies; D001616:beta-Galactosidase; D010751:Phosphopyruvate Hydratase
vernacular_title: Enzym-Immunoassays zur Quantifizierung humaner gamma-gamma-Enolase (NSE).