title: Studies on the hyperplasia ('regeneration') of the rat liver following partial hepatectomy. Changes in lipid peroxidation and general biochemical aspects.
abstract: Using the experimental model of partial hepatectomy in the rat, we have examined the relationship between cell division and lipid peroxidation activity. In rats entrained to a regime of 12 h light/12 h dark and with a fixed 8 h feeding period in the dark phase, partial hepatectomy is followed by a rapid regeneration of liver mass with cycles of synchronized cell division at 24 h intervals. The latter phenomenon is indicated in this study by pulses of thymidine kinase activity having maxima at 24 h, 48 h and 72 h after partial hepatectomy. Microsomes prepared from regenerating livers show changes in lipid peroxidation activity (induced by NADPH/ADP/iron or by ascorbate/iron), which is significantly decreased relative to that in microsomes from sham-operated controls, again at 24 h, 48 h and 72 h after the operation. This phenomenon has been investigated with regard to possible underlying changes in the content of microsomal fatty acids, the microsomal enzymes NADPH:cytochrome c reductase and cytochrome P-450, and the physiological microsomal antioxidant alpha-tocopherol. The cycles of decreased lipid peroxidation activity are apparently due, at least in part, to changes in microsomal alpha-tocopherol content that are closely associated in time with thymidine kinase activity.
authors.affiliation: Department of Biology and Biochemistry, Brunel University, Uxbridge, Middlesex, U.K.
authors.forename: T F
authors.initials: TF
authors.lastname: Slater
authors.forename: K H
authors.initials: KH
authors.lastname: Cheeseman
authors.forename: C
authors.initials: C
authors.lastname: Benedetto
authors.forename: M
authors.initials: M
authors.lastname: Collins
authors.forename: S
authors.initials: S
authors.lastname: Emery
authors.forename: S P
authors.initials: SP
authors.lastname: Maddix
authors.forename: J T
authors.initials: JT
authors.lastname: Nodes
authors.forename: K
authors.initials: K
authors.lastname: Proudfoot
authors.forename: G W
authors.initials: GW
authors.lastname: Burton
authors.forename: K U
authors.initials: KU
authors.lastname: Ingold
mesh_terms: D000818:Animals; D000975:Antioxidants; D002251:Carbon Tetrachloride / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D005227:Fatty Acids / Q000378:metabolism; D006498:Hepatectomy; D006965:Hyperplasia; D050356:Lipid Metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008115:Liver Regeneration; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D010101:Oxygen Consumption; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013937:Thymidine Kinase / Q000378:metabolism; D014810:Vitamin E / Q000378:metabolism
chemical_list: D000975:Antioxidants; D005227:Fatty Acids; D011506:Proteins; D014810:Vitamin E; D004247:DNA; D003577:Cytochrome P-450 Enzyme System; D002251:Carbon Tetrachloride; D009251:NADPH-Ferrihemoprotein Reductase; D013937:Thymidine Kinase