title: New reagents and solid support for automated oligonucleotide synthesis.
abstract: The optimal system for the rapid, efficient, convenient, and economical synthesis and purification of synthetic oligonucleotides has been advancing. By recognizing the very rapid reaction kinetics and taking advantage of an efficient, low volume delivery system, cycle times have decreased to about 5.5 minutes, without compromising synthesis performance. A new set of base protecting groups for cyanoethylphosphoramidite nucleoside monomers have been developed, which decreases the post-synthesis time requirements. A particular form of polystyrene has also been developed as a solid support for automated oligonucleotide synthesis. Typical sequencing or PCR primers (20mers) now require less than 2 hours for synthesis and 2 hours for cleavage and deprotection.
authors.affiliation: Applied Biosystems, Foster City, CA 94404.
authors.forename: H
authors.initials: H
authors.lastname: Vu
authors.forename: C
authors.initials: C
authors.lastname: McCollum
authors.forename: C
authors.initials: C
authors.lastname: Lotys
authors.forename: A
authors.initials: A
authors.lastname: Andrus
mesh_terms: D007202:Indicators and Reagents; D007700:Kinetics; D008722:Methods; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis*; D016133:Polymerase Chain Reaction; D011137:Polystyrenes
chemical_list: D007202:Indicators and Reagents; D009841:Oligonucleotides; D011137:Polystyrenes