Beta-hydroxysteroid dehydrogenase: activity in microemulsion and extraction from Pseudomonas testosteroni cells with microemulsion.
The stability of purified beta-hydroxysteroid dehydrogenase activity measured as a function of time was good in buffered cationic and non-ionic microemulsions. The use of 1-pentanol and 1-hexanol in place of 1-butanol as cosurfactant gave increased activity and stability. The NAD+ Michaelis constant was 0.22 mM in buffer and 3.5 mM in waterpool concentration in microemulsion. Proteins, among them beta-hydroxysteroid dehydrogenase, were extracted from Pseudomonas testosteroni with cationic microemulsion, thus indicating that microemulsions may be utilized in protein release from cells.
Lee
K M
KM
Laboratoire de Chimie Organique Biologique, UA CNRS 31, Université Louis Pasteur, Strasbourg, France.
Biellmann
J F
JF
D004655:Emulsions; D004795:Enzyme Stability; D000441:Hexanols; D006913:Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D007700:Kinetics; D000439:Pentanols; D011549:Pseudomonas / Q000201:enzymology*
D004655:Emulsions; D000441:Hexanols; D000439:Pentanols; C036260:1-hexanol; D006913:Hydroxysteroid Dehydrogenases; C024999:n-pentanol