[Results of endocrinologic studies in culture media after coculture of bovine oocytes with granulosa cells].
The steroid hormones progesterone (P.), and testosterone (T.) were radio-immunologically determined in 108 medium samples, following co-culturing of bovine oocytes with granulosa cells. P. and T. values recorded from a control group were lower with significance than those recorded from co-culturing groups, that is 72 +/- 21 ng/ml and 264 +/- 84 pg/ml as compared to 208 +/- 138 ng/ml and 2,168 +/- 1,595 pg/ml in the oocyte plus fresh granulosa cell co-culturing group as well as 364 +/- 215 ng/ml and 825 +/- 233 pg/ml in the oocyte plus pre-incubated granulosa cell co-culturing group. These rises were accompanied by decline in maturation rate, increase in oocyte degeneration, and rises in the rates of fertilisation and segmentation.
Götze
M
M
Forschungszentrum für Tierproduktion, Akademie der Landwirtschaftswissenschaften, Bereich, Fortpflanzung, Dummerstorf, Rostock.
Gerassimova
G G
GG
Alm
H
H
Makarova
S N
SN
Schneider
F
F
Dmitriev
W B
WB
Kauffold
P
P
Golubjev
A K
AK
D000818:Animals; D002417:Cattle / Q000378:metabolism*; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism*; D011374:Progesterone / Q000032:analysis*; D013739:Testosterone / Q000032:analysis*
D003470:Culture Media; D013739:Testosterone; D011374:Progesterone
Ergebnisse endokrinologischer Untersuchungen in Kulturmedien nach der Kokultur von Rinderoozyten mit Granulosazellen.