[Quantitative determination of apolipoprotein AI in serum by the use of monoclonal antibodies in a noncompetitive ELISA].
A methodical base for estimation of apolipoprotein AI (apo AI) in the serum using a monoclonal antibody in a non-competitive ELISA is described. The technical approach is adjusted to commercial materials of the DDR industry. The assay allows to determine apo AI within the range of 5-100 micrograms apo AI/I. The Vk was 4% (within), and 8% (day to day). The monoclonal antibody used is directed against a native epitope which is fully exposed in the serum. Application of the SUMAL system further improves the practicability of the method. The assay should be suitable particularly for the recognition of epitopes of apo AI in serum HDL fractions associated with an atherogenic risk. The values obtained by the ELISA correspond to those measured turbidimetrically using polyclonal antibodies.
Winkler
L
L
Institut für Pathologische Biochemie, Friedrich-Schiller-Universität Jena.
Müller
D W
DW
Dargel
R
R
Jäger
L
L
D000911:Antibodies, Monoclonal; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*
D000911:Antibodies, Monoclonal; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D008075:Lipoproteins, HDL
Quantitative Bestimmung von Apolipoprotein Al im Serum unter Verwendung eines monoklonalen Antikörpers in einem nichtkompetitiven ELISA.