Immunolocalization of C1-tetrahydrofolate synthase in the rat kidney.
The distribution of the trifunctional enzyme C1-tetrahydrofolate synthase (C1-THF synthase) was examined in the rat kidney by immunolocalization with anti-C1-THF synthase serum using the peroxidase-antiperoxidase method. C1-THF synthase immunoreactivity was detected in both distal and proximal epithelial cells. Staining of the distal tubule epithelia was more intense and granular whereas staining of the proximal tubule epithelia was diffuse. All cells of the cortical collecting duct showed positive granular staining. In the outer medullary collecting duct, the intercalated cells showed intense granular cytoplasmic staining and the principal cells were either negative or weakly positive. The ascending thick limb of Henle's loop was also positive. Glomeruli and the inner medulla showed no staining for C1-THF synthase.
Appling
D R
DR
Clayton Foundation Biochemical Institute, Department of Chemistry, University of Texas, Austin 78712.
Ayo
S H
SH
Kreisberg
J I
JI
D000619:Aminohydrolases / Q000378:metabolism*; D000818:Animals; D005574:Formate-Tetrahydrofolate Ligase / Q000378:metabolism*; D005998:Glycine / Q000378:metabolism; D007158:Immunologic Techniques; D007684:Kidney Tubules / Q000166:cytology / Q000201:enzymology*; D007686:Kidney Tubules, Distal / Q000166:cytology / Q000201:enzymology; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000201:enzymology; D008025:Ligases / Q000378:metabolism*; D008297:Male; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP) / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012694:Serine / Q000378:metabolism
D009097:Multienzyme Complexes; D012694:Serine; C021836:formyl-methenyl-methylenetetrahydrofolate synthetase; D010088:Oxidoreductases; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP); D000619:Aminohydrolases; D008025:Ligases; D005574:Formate-Tetrahydrofolate Ligase; D005998:Glycine