title: Comparison of lysine and tryptophan catabolizing enzymes in rat and bovine tissues.
abstract: Earlier studies indicate that alpha-aminoadipate aminotransferase (AadAT) and kynurenine aminotransferase (KAT) activities from rat tissues are associated with a single protein. However, our recent studies indicate that AadAT activity from bovine liver and kidney is not associated with KAT activity. To test whether the lysine and tryptophan catabolism in bovine tissues differ from that in rat tissues, we compared the activities of enzymes involved in lysine and tryptophan pathways in rat and bovine tissues. The activities of lysine catabolizing enzymes such as AadAT, lysine alpha-ketoglutarate reductase and saccharopine dehydrogenase in the bovine tissues were significantly lower than those found in rat tissues. The activities of tryptophan catabolizing enzymes such as KAT and kynurenine hydroxylase in the bovine tissues were negligible as compared to those in rat tissues. The results suggest that lysine is degraded via the saccharopine pathway in the livers and kidneys of both species but the metabolism of tryptophan in bovine tissues may be different from that in rat tissues.
authors.affiliation: Department of Pediatrics, Wayne State University, Detroit, Michigan 48201.
authors.forename: A
authors.initials: A
authors.lastname: Mukhopadhyay
authors.forename: S M
authors.initials: SM
authors.lastname: Mungre
authors.forename: D R
authors.initials: DR
authors.lastname: Deshmukh
mesh_terms: D051307:2-Aminoadipate Transaminase; D000818:Animals; D002417:Cattle; D006867:Hydrolases / Q000378:metabolism; D007668:Kidney / Q000201:enzymology*; D050601:Kynurenine 3-Monooxygenase; D008099:Liver / Q000201:enzymology*; D008190:Lyases; D008239:Lysine / Q000378:metabolism*; D008297:Male; D006899:Mixed Function Oxygenases / Q000378:metabolism; D051381:Rats; D000637:Transaminases / Q000378:metabolism; D014364:Tryptophan / Q000378:metabolism*; D014366:Tryptophan Oxygenase / Q000378:metabolism
chemical_list: D014364:Tryptophan; D006899:Mixed Function Oxygenases; D014366:Tryptophan Oxygenase; D050601:Kynurenine 3-Monooxygenase; D000637:Transaminases; D051307:2-Aminoadipate Transaminase; C427605:glutamine - phenylpyruvate transaminase; C021481:kynurenine-oxoglutarate transaminase; D006867:Hydrolases; C021480:kynureninase; D008190:Lyases; D008239:Lysine