[Absence of various manifestations of adaptive response in Bacillus subtilis transformants].
Competent cells of Bacillus subtilis are more sensitive to N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) than total population, exhibit higher level of spontaneous mutations to kanamycin resistance. However, the absolute number of mutated transformants doesn't rise with MNNG treatment in the range of 5 to 50 micrograms per ml. The adaptation to low concentrations of MNNG affects neither spontaneous nor MNNG-induced mutagenesis in the competent (transformed) cells, in contrast to their resistance which is stronger for the adapted transformants. The transformation by MNNG-treated plasmid pUB 110 doesn't reveal any difference between adapted and non-adapted cultures in transformation efficiency decline.
Zherebtsov
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SV
D000220:Adaptation, Biological; D001412:Bacillus subtilis / Q000235:genetics* / Q000502:physiology; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D009154:Mutation; D010957:Plasmids; D014169:Transformation, Bacterial
D008769:Methylnitronitrosoguanidine
Otsutstvie nekotorykh proiavleniĭ adaptivnogo otveta v transformantakh Bacillus subtilis.