[The choice of the optimal aeration conditions for the stab cultivation of Bacillus thuringiensis H14 266/2-1].
Optimal conditions of aeration with depth regime of cultivation of Bacillus thuringiensis H14 266/2-1, their effect on the output of biomass, endotoxin and activity of bacteria have been studied. The aeration conditions are optimized using the mathematical method of experiment planning. It is established that the air supply favours an increase of the bacillary biomass and insecticide activity for the whole cultivation period. In the first day of fermentation it is inexpedient to increase to rate of the medium agitation. To obtain a high titre of cells of the studied bacilli and to retain their activity a specific air consumption is recommended amounting to 0.5 1/1/min for the first 12h with an increase to 1.01/1/min in the following hours of fermentation, the constant rate of the medium agitation being 500 rev/min.
Osadchaia
A I
AI
Prokopchenko
S F
SF
Kostiuchenko
I L
IL
Mikhnovskaia
N D
ND
Vasilevskaia
I A
IA
D000330:Aedes; D000332:Aerobiosis; D000818:Animals; D001413:Bacillus thuringiensis / Q000254:growth & development*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000096:biosynthesis; D001427:Bacterial Toxins; D001431:Bacteriological Techniques; D003470:Culture Media; D004731:Endotoxins / Q000096:biosynthesis; D005285:Fermentation; D006460:Hemolysin Proteins; D006863:Hydrogen-Ion Concentration; D009032:Mosquito Control; D013696:Temperature
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003470:Culture Media; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis
Vybor optimal'nykh usloviĭ aérirovaniia dlia glubinnogo kul'tivirovaniia Bacillus thuringiensis H14 266/2-1.