title: Purification of soluble enzymes from erythrocyte hemolysates by three phase partitioning.
abstract: 1. The three phase partitioning method of protein fractionation was successfully applied to human erythrocyte hemolysates for the removal of hemoglobin and the concentration of soluble enzymes. 2. Human carbonic anhydrase I and II, catalase and superoxide dismutase were recovered free of hemoglobin and in good yield in the initial partitioning step, with a 60- to 80-fold enrichment of enzyme activities. 3. After further purification, carbonic anhydrases I and II were obtained at overall yields of 84 and 29%, respectively, crystallized catalase at 38% and superoxide dismutase at 52%.
authors.affiliation: Department of Biochemistry, University of Pretoria, South Africa.
authors.forename: M C
authors.initials: MC
authors.lastname: Pol
authors.forename: H F
authors.initials: HF
authors.lastname: Deutsch
authors.forename: L
authors.initials: L
authors.lastname: Visser
mesh_terms: D002256:Carbonic Anhydrases / Q000097:blood / Q000302:isolation & purification*; D002374:Catalase / Q000097:blood / Q000302:isolation & purification*; D005591:Chemical Fractionation / Q000379:methods; D002853:Chromatography, Liquid; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D012995:Solubility; D013482:Superoxide Dismutase / Q000097:blood / Q000302:isolation & purification*
chemical_list: D002374:Catalase; D013482:Superoxide Dismutase; D002256:Carbonic Anhydrases