Evidence for CD8-independent T cell maturation in transgenic mice.
Double-negative (CD4-/CD8-) T cells expressing the alpha/beta transgenic TCR from the 2C cell line (anti-H-2Ld) were examined in the periphery of animals whose MHC type produces positive, negative, or no selection for differentiation of the TCR on single positive (CD8+) cells. Regardless of the selection haplotype the CD4-/CD8- cells are capable of activation by anticlonotypic mAb indicating that negative selection does not inactivate the "forbidden" TCR. Rather, the lack of response to H-2Ld in the negative haplotype is likely to absence of CD8 required to produce a functional response to H-2Ld. The similarity of surface phenotype and functional activity of these CD4-/CD8- cells maturing in different haplotypes suggests they may arise by an alternative lineage which, unlike the dominant TCR alpha/beta pathway, does not require coexpression of CD4/CD8.
Russell
J H
JH
Department of Pharmacology, Washington University School of Medicine, St. Louis, MO 63110.
Meleedy-Rey
P
P
McCulley
D E
DE
Sha
W C
WC
Nelson
C A
CA
Loh
D Y
DY
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism* / Q000502:physiology*; D000954:Antigens, Surface / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens; D002454:Cell Differentiation; D005434:Flow Cytometry; D006183:H-2 Antigens / Q000032:analysis; D006239:Haplotypes; D008213:Lymphocyte Activation; D008285:Major Histocompatibility Complex; D051379:Mice; D008822:Mice, Transgenic / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000032:analysis; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D015704:CD4 Antigens; D016827:CD8 Antigens; D006183:H-2 Antigens; D015375:Receptors, Interleukin-2