title: Isolation and sequencing of the Alcaligenes denitrificans azurin-encoding gene: comparison with the genes encoding blue copper proteins from Pseudomonas aeruginosa and Alcaligenes faecalis.
abstract: The gene (azu) encoding azurin from Alcaligenes denitrificans has been cloned and sequenced. The gene codes for a pre-protein with a 19-aa signal peptide. Comparison with the sequences coding for the blue copper proteins from Pseudomonas aeruginosa and Alcaligenes faecalis reveals the presence of ntrA and fnr boxes in front of all three genes, instead of a regular [-10, -35]-promoter. In P. aeruginosa, the azu gene is terminated by a bidirectional terminator and flanked by open reading frames on the opposite strand.
authors.affiliation: Gorlaeus Laboratories, Leiden University, The Netherlands.
authors.forename: C W
authors.initials: CW
authors.lastname: Hoitink
authors.forename: L P
authors.initials: LP
authors.lastname: Woudt
authors.forename: J C
authors.initials: JC
authors.lastname: Turenhout
authors.forename: M
authors.initials: M
authors.lastname: van de Kamp
authors.forename: G W
authors.initials: GW
authors.lastname: Canters
mesh_terms: D000421:Alcaligenes / Q000235:genetics*; D000595:Amino Acid Sequence; D001400:Azurin / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping
chemical_list: D001426:Bacterial Proteins; D001400:Azurin