Comparative evaluation of three tests for the detection of Escherichia coli cytotoxic necrotizing factors (CNF1 and CNF2) using filtrates of cultures treated with mitomycin C.
Necrotizing Escherichia coli (NTEC) strains grown in the presence of mitomycin C released cell associated necrotizing factors CNF1 and CNF2 to culture medium. Using culture filtrates from 96 mitomycin C treated E. coli strains, we have found that a modified HeLa cell assay was a more sensitive and specific method for the detection of CNF1 and CNF2 than the Vero cell assay and the rabbit skin test.
Blanco
J
J
Departamento de Microbiología y Parasitología, Facultad de Veterinaria, Universidad de Santiago, Lugo, Spain.
Blanco
M
M
González
E A
EA
Alonso
M P
MP
Garabal
J I
JI
D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis*; D003470:Culture Media; D003603:Cytotoxins / Q000032:analysis*; D004926:Escherichia coli / Q000032:analysis* / Q000187:drug effects; D029968:Escherichia coli Proteins; D006367:HeLa Cells; D006801:Humans; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology*; D011817:Rabbits; D012882:Skin Tests; D014709:Vero Cells
D001427:Bacterial Toxins; D003470:Culture Media; D003603:Cytotoxins; D029968:Escherichia coli Proteins; D008937:Mitomycins; C060296:cytotoxic necrotizing factor type 1; D016685:Mitomycin