Probing salt-induced and supercoiling-induced B-Z junctions in DNA by bisulfitemethoxyamine.
Salt-induced and supercoiling-induced B-Z junctions in pWR756, a plasmid containing (GC)16, were probed with bisulfite-methoxyamine, a modification reagent specific for single-stranded nucleic acids. The modification sites were analyzed with S1 nuclease and the modified cytosines were determined from termination sites of DNA chain elongation by DNA polymerase. The results showed that most accessible cytosines are the same for both types of B-Z junctions.
Negishi
K
K
Gene Research Center, Okayama University, Japan.
Yamashita
T
T
Zacharias
W
W
Wells
R D
RD
Bessho
T
T
Hayatsu
H
H
D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000378:metabolism; D004278:DNA, Superhelical / Q000737:chemistry* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D006898:Hydroxylamines / Q000737:chemistry; D015394:Molecular Structure; D012492:Salts / Q000737:chemistry*; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism; D013447:Sulfites / Q000737:chemistry
D004277:DNA, Single-Stranded; D004278:DNA, Superhelical; D006898:Hydroxylamines; D012492:Salts; D013447:Sulfites; C005214:methoxyamine; D015719:Single-Strand Specific DNA and RNA Endonucleases; C042345:hydrogen sulfite