title: High molecular weight aggregate content of heated and unheated factor VIII products determined by fast-protein liquid chromatography.
abstract: The molecular-weight distribution of proteins in factor VIII concentrates has been analysed by fast-protein liquid chromatography. The proportion of high-molecular-weight (HMW) aggregates in one product increased on freeze-drying and heating, with fibrinogen and fibronectin being the main protein components of the HMW peak. In all other concentrates, the HMW peak was less than or equal to 5% of the total protein content and there were no differences in HMW content according to purity or method of viral inactivation.
authors.affiliation: Heart Research Institute, Sydney, Australia.
authors.forename: J
authors.initials: J
authors.lastname: Dawes
authors.forename: L
authors.initials: L
authors.lastname: Freeman
authors.forename: N J
authors.initials: NJ
authors.lastname: Dawson
authors.forename: D S
authors.initials: DS
authors.lastname: Pepper
authors.forename: T W
authors.initials: TW
authors.lastname: Barrowcliffe
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D005169:Factor VIII / Q000032:analysis*; D006358:Hot Temperature; D006801:Humans; D008970:Molecular Weight
chemical_list: D005169:Factor VIII