[Factor-free translation of poly(dT) by 80S ribosomes from Saccharomyces cerevisiae].
The conditions for preparation of 80S ribosomes from S. cerevisiae are suggested. The ribosomes can bind Phe-tRNAPhe in poly(U)-or poly(dT)-directed manner and are shown to be able to translate poly(dT) in the absence of elongation factor and GTP. Effects of different antibiotics on the factor-free translation have been studied.
Ovcharenko
G V
GV
Potapov
A P
AP
El'skaia
A V
AV
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002853:Chromatography, Liquid; D006160:Guanosine Triphosphate / Q000378:metabolism; D007700:Kinetics; D010445:Peptide Elongation Factors / Q000378:metabolism; D011071:Poly T / Q000235:genetics*; D014176:Protein Biosynthesis / Q000187:drug effects; D012360:RNA, Transfer, Phe / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D010445:Peptide Elongation Factors; D012360:RNA, Transfer, Phe; D011071:Poly T; D006160:Guanosine Triphosphate
Besfaktornaia transliatsiia poli(dT) 80S ribosomami iz drozhzheĭ Saccharomyces cerevisiae.