The effects of prenatal protein-energy malnutrition on ossification of fetal rat bones: a biochemical study.
In fetal rats whose dams were fed a low-protein diet, 35S sulfate uptake into the growth plate of the long bone and rib was higher than in the control group. The elution pattern of guanidine-HCl extract in gel chromatography revealed that the malnourished group had more high molecular weight proteoglycans in the dissociative condition and a larger aggregated portion in the associative condition than did the control group; however, the same chondroitin-sulfate chain size existed. Calcium content did not differ in both groups. Aggregated proteoglycan or a high molecular weight proteoglycan that existed in the malnourished group probably played an inhibitory role in calcification. Prenatal protein-energy malnutrition may delay the change of proteoglycan character, which could affect mineralization of fetal bones.
Miwa
T
T
Department of Orthopedic Surgery, Louisiana State University Medical Center, New Orleans 70112.
Shoji
H
H
Solomonow
M
M
Nakamoto
T
T
D000818:Animals; D001724:Birth Weight; D001835:Body Weight; D001848:Bone Diseases, Developmental / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology; D005260:Female; D005315:Fetal Diseases / Q000209:etiology / Q000378:metabolism* / Q000503:physiopathology; D010012:Osteogenesis / Q000502:physiology; D011247:Pregnancy; D011248:Pregnancy Complications; D011502:Protein-Energy Malnutrition / Q000150:complications*; D011509:Proteoglycans / Q000378:metabolism / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains
D011509:Proteoglycans