Enhancement of T lymphocyte proliferation and suppression of antibody producing cell formation by methionine-enkephalin.
Methionine-enkephalin (met-enk) 0.1-100 nmol/L significantly enhanced lymphocyte proliferation induced by T cell mitogens. On the other hand, the peptide markedly inhibited splenocyte blastogenesis induced by B cell mitogen lipopolysaccharides (LPS) and sheep red blood cell (SRBC)-driven plaque-forming cell (PFC) formation in vitro. Met-enk alone had no effect on immune responses. However, naloxone 50 nmol/L had also a stimulating effect on Con A-induced splenocyte proliferation. The similar results were also observed in vivo. The results also indicated that the enhancement of T cell function by met-enk was stronger in immunosuppressed mice than in the normal mice.
Yang
S X
SX
Shanghai Institute of Materia Medica, Chinese Academy of Sciences.
Li
X Y
XY
D000818:Animals; D000921:Antibody-Producing Cells / Q000187:drug effects*; D003208:Concanavalin A / Q000494:pharmacology; D003524:Cyclosporins / Q000494:pharmacology; D004744:Enkephalin, Methionine / Q000494:pharmacology*; D005260:Female; D006462:Hemolytic Plaque Technique; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D009270:Naloxone / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*
D003524:Cyclosporins; D003208:Concanavalin A; D009270:Naloxone; D004744:Enkephalin, Methionine