Interferon-gamma regulation of major histocompatibility class I gene expression in rat cells containing the adenovirus 12 E1A oncogene.
Interferon-gamma (IFN gamma) treatment of cells expressing the Ad12 E1A oncogene results in an increase in MHC class I antigen expression. The effect of IFN gamma upon both the steady-state level of class I mRNA and the in vitro rate of initiation of transcription of class I genes was studied in a rat cell line containing the Ad12 E1A gene. Results obtained showed a marked increase in steady-state level of cytoplasmic class I mRNA; however, a concomitant increase in the rate of transcription of class I genes was not observed. In contrast IFN gamma was shown to markedly increase the rate of transcription of class I genes in primary baby rat kidney cells.
Ackrill
A M
AM
Department of Biochemistry, University of Leeds, United Kingdom.
Blair
G E
GE
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D001345:Autoradiography; D015152:Blotting, Northern; D002461:Cell Line, Transformed; D003720:Densitometry; D015967:Gene Expression Regulation, Viral; D005805:Genes, MHC Class I; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis / Q000235:genetics*; D007371:Interferon-gamma / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D014158:Transcription, Genetic
D015395:Histocompatibility Antigens Class I; D012333:RNA, Messenger; D007371:Interferon-gamma