Crystallization and preliminary x-ray analysis of soybean lipoxygenase-1, a non-heme iron-containing dioxygenase.
Crystals of lipoxygenase-1 from soybeans have been grown by the method of vapor diffusion in the presence of sodium formate, ammonium acetate, and lithium chloride at pH 7.0. This enzyme contains a non-heme iron and is closely related to a human lipoxygenase found in leukocytes that participates in the biosynthesis of leukotrienes and lipoxins. The crystals are monoclinic space group C2 with cell dimensions of a = 183.8 A, b = 123.2 A, c = 94.3 A and beta = 102.9 degrees. They diffract beyond 2.7 A, are stable for several days in the x-ray beam, and appear to be suitable for x-ray diffraction studies.
Boyington
J C
JC
Department of Biophysics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Gaffney
B J
BJ
Amzel
L M
LM
D002851:Chromatography, High Pressure Liquid; D003460:Crystallization; D008084:Lipoxygenase / Q000302:isolation & purification; D013025:Glycine max / Q000201:enzymology*; D014961:X-Ray Diffraction
D008084:Lipoxygenase