Maintenance of energy-linked functions in rat liver mitochondria.
EGTA and EDTA are compared with respect to their ability to preserve ATP-requiring reactions in rat liver mitochondria. The presence of EGTA sustains the high ATP requirement of citrulline synthesis. EDTA does not, even with excess Mg2+. Carboxylation of pyruvate, which has a lower ATP demand, is not influenced by the type of chelating agent. In mitochondria stored for 24 h at 4 degrees C, EGTA is more effective than EDTA in preventing loss of these energy-linked functions.
Lund
P
P
Nuffield Department of Clinical Medicine, Radcliffe Infirmary, Oxford, U.K.
Wiggins
D
D
D000227:Adenine Nucleotides / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002956:Citrulline / Q000096:biosynthesis; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D004734:Energy Metabolism; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid; D051381:Rats; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid; D014021:Tissue Preservation
D000227:Adenine Nucleotides; D011773:Pyruvates; D013386:Succinates; D002956:Citrulline; D004533:Egtazic Acid; D019289:Pyruvic Acid; D000255:Adenosine Triphosphate; D004492:Edetic Acid; D019802:Succinic Acid; D008274:Magnesium