Constraction of a subtracted cDNA library using oligo(dT)-latex.
An efficient method for constraction of subtracted cDNA library was developed using oligo(dT 30. Latex and PCR. This method improved the chances for identifying cDNA clones corresponding to scarce class of mRNA that is expressed differentially during cellular growth and differentiation.
Hara
E
E
Department of Applied Biological Science, Science University of Tokyo, Chiba, Japan.
Kato
T
T
Nakada
S
S
Nakajima
T
T
Tsurui
H
H
Oda
K
K
D000081:Acetamides; D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA; D015723:Gene Library; D006801:Humans; D007840:Latex; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D000081:Acetamides; D007840:Latex; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; C027903:oligo (dT); D004247:DNA; C014026:hexamethylene bisacetamide