[Staphylococcus aureus (STA), a B- and T-cell mitogen].
Mitogenesis in peripheral blood lymphocyte and separated T and non-T cells cultures activated with formalin-fixed and heated staphylococcus aureus strain 520 (STA) was studied. The peak of DNA-synthesis in presence of STA is on day 7. The maximum of proliferation was found for T cell on day 7 and for non-T cells on day 3 after stimulation with STA. We found a strong correlation between the expression of CD25 antigen and the 3H-thymidine incorporation. It is concluded that B and T cell proliferation can be studied without purifying the cells using the different proliferation kinetics in presence of STA.
Mansfield
H W
HW
Forschungsabteilung Experimentelle Immunologie, Medizinischen Akademie Magdeburg.
Ansorge
S
S
D001402:B-Lymphocytes / Q000276:immunology*; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008934:Mitogens; D010835:Phytohemagglutinins / Q000494:pharmacology; D013205:Staphylococcal Protein A / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D003470:Culture Media; D008934:Mitogens; D010835:Phytohemagglutinins; D013205:Staphylococcal Protein A; D004247:DNA
Staphylococcus aureus (STA), ein B- und T-Zell-Mitogen.