[Measurement of gamma-interferon in sera and CSF in patients with multiple sclerosis and inflammatory neurological diseases].
We examined whether gamma-interferon (gamma-IFN) can be detected in serum and CSF of patients with multiple sclerosis and other inflammatory neurological diseases. Gamma-IFN was assayed by solid phase radioimmunoassay on the forward sandwich principle. In 7 serum samples in acute stage of multiple sclerosis without corticosteroid, in 2 CSF samples in acute stage, gamma-IFN was not detected. In stable stage there was no case with positive gamma-IFN. The patient with tuberculous meningitis showed high titer in CSF but not in serum. One case with herpes zoster meningitis, one case out of 2 aseptic encephalitis showed positive gamma-IFN in CSF. In one case with Vogt-Koyanagi-Harada disease, gamma-IFN was detected both in serum and CSF. One case with Neuro-Behçet syndrome showed positive gamma-IFN in CSF. No gamma-IFN was detected in 2 cases with Guillain-Barré syndrome, one case with Crow-Fukase syndrome, Fisher syndrome, 2 cases with polymyosits. gamma-IFN in CSF was detected in meningitis and encephalitis, but not in serum. This suggests that the locally infiltrating cells produce gamma-IFN. However, we could not detect gamma-IFN in either CSF or serum of patients with multiple sclerosis. Negative results of gamma-IFN in patients with multiple sclerosis can be interpreted in 2 ways. 1. The half life of gamma-IFN is very short in vivo, and the level of gamma-IFN may not be detected at the time of sampling. 2. Generalized augmentation of gamma-IFN production may not be observed but locally infiltrating cells or astrocytes might produce gamma-IFN.(ABSTRACT TRUNCATED AT 250 WORDS)
Hirayama
M
M
Second Department of Internal Medicine, Fukui Medical School.
Kiyosawa
K
K
Nakazaki
S
S
Fujiki
N
N
Iida
M
M
D001528:Behcet Syndrome / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000097:blood* / Q000134:cerebrospinal fluid; D009103:Multiple Sclerosis / Q000378:metabolism*; D011129:Polyradiculoneuropathy / Q000378:metabolism*
D007371:Interferon-gamma