A 165 kDa membrane antigen mediating fibronectin-vinculin interaction is involved in murine odontoblast differentiation.
Membrane-mediated matrix-microfilament interactions are involved in odontoblast differentiation. In this study, we analyzed the interactions of vinculin and fibronectin with plasma membrane proteins separated by sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis, and then transferred onto polyvinylidene-difluoride (PVDF) paper. Vinculin was found to interact with 58, 63 and 165 kDa plasma membrane proteins. Fibronectin interacted with three high molecular weight (145, 165, and 185 kDa) membrane proteins. Attempts were made to characterize the 165 kDa protein which interacted with vinculin and with fibronectin. The interaction of the 165 kDa protein with fibronectin was not competitively inhibited by synthetic peptides such as GRGDS or GRGDSP, suggesting that the protein was not related to integrins. Antibodies directed against the 165 kDa protein allowed the identification of the precise localization and biological role of this membrane antigen. The data presented in this paper and previous observations indicate that the 165 kDa protein, involved in odontoblast elongation and polarization, mediates a fibronectin-vinculin transmembrane interaction.
Lesot
H
H
Institut de Biologie Médicale, INSERM CJF 88-08, Faculté de Médecine, Strasbourg, France.
Kubler
M D
MD
Fausser
J L
JL
Ruch
J V
JV
D000199:Actins / Q000378:metabolism; D000818:Animals; D000917:Antibody Formation / Q000502:physiology; D000954:Antigens, Surface / Q000276:immunology*; D015153:Blotting, Western; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005353:Fibronectins / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D016023:Integrins / Q000032:analysis; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D009804:Odontoblasts / Q000166:cytology*; D016596:Vinculin
D000199:Actins; D000954:Antigens, Surface; D003598:Cytoskeletal Proteins; D005353:Fibronectins; D016023:Integrins; D008565:Membrane Proteins; D016596:Vinculin