[The conjugative transfer of beta-hemolysin plasmid in Enterococcus faecalis to Enterococcus faecium].
The conjugative transfer by using a filter mating method in 109 cases of combinations between 20 strains of beta-hemolytic Enterococcus faecalis and 28 strains of alpha-hemolytic Enterococcus faecium was tried. A stable transconjugation was observed in two cases: from E. faecalis No. 170 to E. faecium No. 49 and from E. faecalis No. 500 to E. faecium No. 48. From the results, it was proved that the beta-hemolysin plasmid of E. faecalis is transferred by conjugation to E. faecium. The virulence (LD50) of E. faecium No. 17049, No. 50048 (heterogeneous transconjugant) and E. faecalis No. 17067, No. 50067 (homologous transconjugant) in mice was compared with their parent strains. The virulence of E. faecalis transconjugants was more marked than that of the recipients. However, the virulence of E. faecium transconjugants was not enhanced. This fact suggests that, only in E. faecalis, does beta-hemolysin play a role in its pathogenicity in mice. beta-Hemolysin in E. faecium transconjugants does not participate in pathogenicity in mice. It may be due to the fact that it takes more time to cause beta-hemolysis on blood agar plate or beta-hemolysin production in B-YEG broth by E. faecium transconjugants than by E. faecalis transconjugants.
Ohno
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Department of Microbiology, School of Medicine, Toho University.
D000818:Animals; D003227:Conjugation, Genetic; D004247:DNA; D013293:Enterococcus faecalis / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D006460:Hemolysin Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000633:toxicity; D007928:Lethal Dose 50; D051379:Mice; D010957:Plasmids / Q000235:genetics; D014774:Virulence
D006460:Hemolysin Proteins; D004247:DNA