Alternative splicing of the Evi-1 zinc finger gene generates mRNAs which differ by the number of zinc finger motifs.
Evi-1 is a common integration site for endogenous ecotropic virus in myeloid leukemias of the AKXD mouse strain. This gene encodes a zinc finger protein with ten finger motifs. Myeloblastic leukemias obtained after infection with F-MuLV frequently exhibit proviral integration in the Fim-3 region which is genetically linked to Evi-1. This proviral integration always results in the expression of two mRNA transcripts, 5.7 kb and 4.7 kb long. We isolated two classes of cDNAs from a myeloblastic cell line with a F-MuLV provirus integrated in Fim-3. By sequence analysis, we found that one Evi-1 cDNA (E29) had an internal deletion of 972 nucleotides when compared to the full-length sequence previously published by Morishita et al. (1988). This deletion eliminated the 6th and 7th Evi-1 finger domains and was bordered by consensus donor and acceptor splice sequences. The E29 clone most likely resulted from alternative splicing of the Evi-1 gene. This was confirmed by Northern blot analysis and S1 mapping experiments. Therefore, the Evi-1 gene codes potentially for at least two proteins of 1042 and 718 amino acids differing in the numbers of their zinc-finger motifs.
Bordereaux
D
D
Laboratoire Immunologie et Oncologie des Maladies Rétrovirales, INSERM U152, Hôpital Cochin, Paris, France.
Fichelson
S
S
Tambourin
P
P
Gisselbrecht
S
S
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D007942:Leukemia, Experimental / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D007951:Leukemia, Myeloid / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D008667:Metalloproteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D008667:Metalloproteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm