Analysis of the rat JE gene promoter identifies an AP-1 binding site essential for basal expression but not for TPA induction.
We have cloned the immediate-early serum-reponsive JE gene from the rat in order to study the regulation of this gene. We show that sequences of the JE promoter region confer serum-inducibility to a reporter gene. Analysis of the promoter in transient assays reveals that: i) the -141/-88 region is required for the response to the phorbol ester TPA, ii) the -70/-38 region is essential for basal activity. This latter region harbors the sequence TGACTCC, which resembles the consensus site for AP-1 binding, TGACTCA. DNA-protein binding assays indicate that the JE AP-1 site and the consensus AP-1 site have an overlapping but not identical binding spectrum for AP-1 proteins. Our data suggest that the inability of some AP-1 sites to respond to TPA is caused by subtle differences in affinity for AP-1 proteins.
Timmers
H T
HT
Laboratory for Molecular Carcinogenesis, Sylvius Laboratories, University of Leiden, The Netherlands.
Pronk
G J
GJ
Bos
J L
JL
van der Eb
A J
AJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018932:Chemokine CCL2; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006023:Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D016755:Proto-Oncogene Proteins c-jun; D012326:RNA Splicing; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic
D018932:Chemokine CCL2; D004268:DNA-Binding Proteins; D006023:Glycoproteins; D016755:Proto-Oncogene Proteins c-jun; D014157:Transcription Factors; D013755:Tetradecanoylphorbol Acetate