Search for novel RNA catalysts. An RNA component with oxidoreductase activity.
A novel RNA component with oxidoreductase activity (diaphorase activity) has been purified from an RNA fraction of Torula yeast. The RNA component was obtained in a 0.05% yield by a series of steps, SDS-phenol extraction, nuclease P1 digestion, alkaline phosphatase digestion, anion exchange chromatography, and HPLC on an ODS-column.
Yanagawa
H
H
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Ogawa
Y
Y
Ueno
M
M
Sasaki
K
K
Sato
T
T
D000469:Alkaline Phosphatase / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003454:Cryptococcus / Q000201:enzymology*; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism*; D016337:RNA, Catalytic / Q000302:isolation & purification / Q000378:metabolism*; D012331:RNA, Fungal / Q000302:isolation & purification / Q000378:metabolism
D016337:RNA, Catalytic; D012331:RNA, Fungal; D008058:Dihydrolipoamide Dehydrogenase; D000469:Alkaline Phosphatase