Development of 1-cell embryos from different strains of mice in CZB medium.
One-cell embryos from several different strains of mice have been cultured to the blastocyst stage in CZB medium. CZB medium can be used to culture CF1 x B6SJLF1/J 1-cell embryos to the blastocyst stage provided glucose is introduced into the medium on Day 3 of culture. The amount of glucose required for embryo development was titrated using a concentration range of 5.5 to 49.5 mM. With the exception of the highest concentration, all glucose levels tested supported 65-85% development to the morula and blastocyst stages. Variations of CZB medium were tested for their ability to support the development of 1-cell embryos from 4 strains of mice. For embryos from CF1 and DBA/2J (both x B6SJLF1/J) mice, which exhibit a "2-cell block" to development in vitro, CZB medium containing glutamine with the addition of glucose on Day 3 supported optimum development from the 1-cell stage to morula and blastocysts (79% and 87%). For embryos from B6D2F1/J and CD1 female mice (both x B6SJLF1/J males), which do not exhibit a "2-cell block" to in vitro development, optimum development to morula and blastocyst stages (95% and 50%) was in CZB medium containing both glutamine and glucose from the start of culture.
Chatot
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CL
Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545.
Lewis
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JL
Torres
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Ziomek
C A
CA
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000032:analysis / Q000494:pharmacology*; D004492:Edetic Acid / Q000032:analysis / Q000494:pharmacology; D004622:Embryo, Mammalian / Q000166:cytology* / Q000187:drug effects; D005260:Female; D005947:Glucose / Q000008:administration & dosage / Q000494:pharmacology; D005973:Glutamine / Q000032:analysis / Q000494:pharmacology; D007267:Injections; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000494:pharmacology
D003470:Culture Media; D005973:Glutamine; D012710:Serum Albumin, Bovine; D004492:Edetic Acid; D005947:Glucose