Isolation of a Bacillus subtilis mutant defective in constitutive O6-alkylguanine-DNA alkyltransferase.
A mutant of Bacillus subtilis defective in the constitutive activity of O6-alkylguanine-DNA alkyltransferase was isolated from a strain (ada-1) deficient in the adaptive response to DNA alkylation. Cells carrying the mutation dat-1 which was responsible for the defect in constitutive activity exhibited hypersensitivity for lethality and mutagenesis when challenged with methyl-nitroso compounds. The constitutive activity is independent of the adaptive response, and seems to function as a basal defense against environmental alkylating agents.
Morohoshi
F
F
Radiobiology Division, National Cancer Center Research Institute, Tokyo, Japan.
Munakata
N
N
D019883:Alkyl and Aryl Transferases; D000477:Alkylating Agents / Q000633:toxicity; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D002470:Cell Survival / Q000187:drug effects; D004260:DNA Repair; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D008780:Methyltransferases / Q000378:metabolism*; D009153:Mutagens; D009154:Mutation; D019853:O(6)-Methylguanine-DNA Methyltransferase; D014166:Transferases / Q000378:metabolism
D000477:Alkylating Agents; D009153:Mutagens; D014166:Transferases; D008780:Methyltransferases; D019853:O(6)-Methylguanine-DNA Methyltransferase; D019883:Alkyl and Aryl Transferases; C050956:DNA alkyltransferase