title: brlA requires both zinc fingers to induce development.
abstract: Expression of the Aspergillus nidulans brlA gene induces a developmental pathway leading to the production of asexual spores. We have introduced mutations into brlA that are expected to disrupt either or both Cys2-His2 Zn(II) coordination sites postulated to exist in the BrlA polypeptide. The resultant brlA alleles fail to induce either the asexual reproductive pathway or the expression of development-specific genes. These data support the hypothesis that brlA encodes a nucleic acid-binding protein whose activity requires each of two zinc fingers.
authors.affiliation: Department of Genetics, University of Georgia, Athens 30602.
authors.forename: T H
authors.initials: TH
authors.lastname: Adams
authors.forename: H
authors.initials: H
authors.lastname: Deising
authors.forename: W E
authors.initials: WE
authors.lastname: Timberlake
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001233:Aspergillus nidulans / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000502:physiology; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal; D008667:Metalloproteins / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D012689:Sequence Homology, Nucleic Acid; D013172:Spores, Fungal / Q000166:cytology / Q000502:physiology; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors / Q000235:genetics; D014982:Xenopus laevis; D015032:Zinc / Q000378:metabolism*
chemical_list: C063497:BRLA protein, Emericella nidulans; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D008667:Metalloproteins; D015345:Oligonucleotide Probes; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors; D015032:Zinc