title: Expression of a rice glutelin promoter in transgenic tobacco.
abstract: A chimeric gene consisting of the 5' flanking sequences of a rice glutelin gene (Gt3) linked to the chloramphenicol acetyltransferase (CAT) coding segment was introduced into tobacco via Agrobacterium tumefaciens-mediated transformation. CAT enzyme activity could be detected in extracts from seeds as early as 8 days after flowering and obtained a maximum level at 16 days after flowering, the onset of overall protein accumulation. Significant expression of CAT activity in non-seed tissues occurred in some, but not all plants, suggesting possible chromosome position effects on non-seed tissue expression. A positive correlation was observed between expression levels in seeds and gene copy numbers.
authors.affiliation: Institute of Biological Chemistry, Washington State University, Pullman 99164-6340.
authors.forename: D J
authors.initials: DJ
authors.lastname: Leisy
authors.forename: J
authors.initials: J
authors.lastname: Hnilo
authors.forename: Y
authors.initials: Y
authors.lastname: Zhao
authors.forename: T W
authors.initials: TW
authors.lastname: Okita
mesh_terms: D002678:Chimera; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D017343:Genes, Plant; D005983:Glutens / Q000235:genetics*; D007700:Kinetics; D012275:Oryza / Q000235:genetics*; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D012231:Rhizobium / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D005983:Glutens; D015500:Chloramphenicol O-Acetyltransferase