[Subcellular distribution of glutathione S-transferase in Chinese fetal liver].
Subcellular fractions were isolated from Chinese fetal liver at 4-8 months of age for the determination of glutathione S-transferase (GST). Using 1-chloro-2,4-dinitrobenzene (CDNB) as substrate, GST activity was found to be 66 +/- 34 nmol/(min.mg protein), mainly in the cytosol. The GST activities were detected principally in microsomes and their values were 66 +/- 31 and 144 +/- 83 nmol/(min.mg protein), respectively, when assayed with p-nitrobenzyl chloride (PNB) and ethacrynic acid (EA) as substrates. There were no age and sex-related differences in GST activities for any of the substrates studied during fetal development. The Km values of GST for CDNB, PNB and EA were 1112, 1039 and 205 mumol/L, respectively. The conjugation of GST may play an important role in fetal hepatic metabolism of toxic electrophiles.
Lei
S B
SB
Department of Pharmacology, Hubei Medical College, Wuchang, China.
Peng
R X
RX
D003600:Cytosol / Q000201:enzymology; D004137:Dinitrochlorobenzene; D004976:Ethacrynic Acid; D005333:Fetus; D005982:Glutathione Transferase / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008862:Microsomes, Liver / Q000201:enzymology; D009578:Nitrobenzenes; D013379:Substrate Specificity
D004137:Dinitrochlorobenzene; D009578:Nitrobenzenes; C027562:4-nitrobenzyl chloride; D005982:Glutathione Transferase; D004976:Ethacrynic Acid