Photodynamic therapy of choriocarcinoma transplanted to the hamster cheek pouch. I. Intraperitoneal photosensitization.
Human choriocarcinoma (JEG-3) cells were transplanted into the cheek pouch of hamsters and treated with photodynamic therapy. Twenty-four hours after intraperitoneal injection of the photosensitizer dihematoporphyrin ether (DHE), 20 tumors were illuminated with 100 J/cm2 of 630-nm light from an argon pumped dye laser. Contralateral tumors served as controls. Dihematoporphyrin ether alone had no effect on tumor growth, while laser light in the absence of DHE resulted in complete regression in 3 tumors (17%), and partial regression in 4 of 18 tumors (22%), possibly due to hyperthermia, P greater than 0.10. Using the combination of DHE plus light (photodynamic therapy) complete tumor regression was noted after a single treatment in 11 of 20 tumors (55%, mean tumor volume 279 mm3) and in 7 of 7 tumors (100%) after a second treatment. Two of 20 tumors were not retreated. Therefore, 18 of 20 tumors (90%) were grossly destroyed by one or two photodynamic treatments. Contralateral control tumors continued to grow to a median volume of 990 mm3 (chi 2 = 26.30, P less than 0.0001). Choriocarcinoma transplanted into the hamster cheek pouch is highly responsive to photodynamic therapy.
Brand
E
E
Department of Obstetrics and Gynecology, Cedars-Sinai Medical Center, Los Angeles, California 90048.
Choi
H S
HS
Karalis
K
K
Papaioannou
T
T
Fishbein
M C
MC
Braunstein
G
G
Wade
M E
ME
Lagasse
L D
LD
Grundfest
W S
WS
D000818:Animals; D002610:Cheek; D002822:Choriocarcinoma / Q000188:drug therapy* / Q000473:pathology / Q000628:therapy; D006224:Cricetinae; D017323:Dihematoporphyrin Ether; D006415:Hematoporphyrins / Q000627:therapeutic use*; D006801:Humans; D007274:Injections, Intraperitoneal; D053685:Laser Therapy; D008647:Mesocricetus; D009336:Necrosis; D009368:Neoplasm Transplantation; D010778:Photochemotherapy; D010789:Phototherapy; D012983:Soft Tissue Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000628:therapy
D006415:Hematoporphyrins; D017323:Dihematoporphyrin Ether