title: Isomyosin distributions in rodent muscles: effects of altered thyroid state.
abstract: In this study we examined the effects of 6-8 wk of thyroid hormone manipulation on striated muscle isomyosin expression in adult female rats. Animals were randomly assigned to one of three groups: 1) euthyroid controls, 2) thyroid deficient (propylthiouracil treated), and 3) hyperthyroid (triiodothyronine treated). Thyroid deficiency resulted in a marked increase in the low-adenosinetriphosphatase V3 isoform by 20- and 49-fold in the left and right ventricle, respectively. Conversely, hyperthyroidism induced a modest (3-11%) but significant increase in the high-adenosinetriphosphatase V1 isoform in both ventricles. The thyroid-deficient rats exhibited significant increases in slow myosin in both soleus (8%) and red gastrocnemius (24%), with concomitant reductions in intermediate myosin in both muscles. Interestingly, while the slow-myosin isoform was decreased in both the soleus (-19%) and the red gastrocnemius (-43%) of the hyperthyroid group, the intermediate-myosin isoform was affected differentially in the two muscles, with a fivefold increase in the former vs. a 16% decrease in the latter. Furthermore, hyperthyroidism increased the fast myosins in the red gastrocnemius while exerting no effect on the same isoforms in the white gastrocnemius. Collectively these data suggest both different specificity and sensitivity among the myosin genes of different striated muscle types in response to thyroid hormone.
authors: Department of Physiology and Biophysics, University of California, Irvine 92717.
authors: D P
authors: DP
authors: Fitzsimons
authors: R E
authors: RE
authors: Herrick
authors: K M
authors: KM
authors: Baldwin
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D005260:Female; D006980:Hyperthyroidism / Q000378:metabolism; D007037:Hypothyroidism / Q000378:metabolism; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D009218:Myosins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013961:Thyroid Gland / Q000502:physiology*; D014018:Tissue Distribution; D014284:Triiodothyronine / Q000494:pharmacology
chemical_list: D014284:Triiodothyronine; D000251:Adenosine Triphosphatases; D009218:Myosins