Plasminogen activator (PA) in Guerin epithelioma. Additional PA inhibitor in plasma of rats bearing the epithelioma.
The presence of plasminogen activator (PA) with a Mr of about 35,000 was detected by SDS-PAGE in extract from Guerin epithelioma. The activator, which is a serine proteinase, was partially purified on Sephadex G-50 followed by Lys-Sepharose. Rat plasma, both of healthy and epithelioma-bearing animals, inhibited the activity of such isolated PA. However, the difference between these plasmas in their antiactivator action was observed after inactivation of plasma proteinase inhibitors. In such conditions, the control plasma lost the ability to inhibit the examined PA, whereas the plasma of epithelioma bearing rats retained this ability.
Tomasiuk
R
R
Medical Academy in Lódz, Institute of Environmental Research and Bioanalysis, Department of Biochemistry, Poland.
Mirowski
M
M
Wierzbicki
R
R
D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D001550:Benzamidines / Q000494:pharmacology; D002277:Carcinoma / Q000097:blood*; D002845:Chromatography; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005341:Fibrinolysin / Q000502:physiology; D066298:In Vitro Techniques; D010960:Plasminogen Activators / Q000097:blood*; D015849:Plasminogen Inactivators / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012697:Serine Endopeptidases / Q000502:physiology
D000814:Aniline Compounds; D001550:Benzamidines; D015849:Plasminogen Inactivators; C008625:nitroaniline; D004492:Edetic Acid; D010960:Plasminogen Activators; D012697:Serine Endopeptidases; D005341:Fibrinolysin; C032157:benzamidine