PMN-derived proteases enhance the affinity of Fc gamma receptor II on myeloid cells, but not on B cells.
Treatment of monocytes or K562 cells with proteolytic enzymes like pronase or trypsin, increases both the affinity of the type II Fc receptor for IgG and the signaling via this receptor. In the present study we evaluated whether other proteases could similarly enhance Fc gamma RII affinity. We furthermore assessed whether all cell types expressing Fc gamma RII display this effect. Therefore, proteins from the coagulation system and PMN-derived enzymes were tested for effects on Fc gamma RII-mediated ligand binding. Enzymes of the coagulation system were tested both in fibrinogen-depleted plasma, as well as in purified form. No effects were found on Fc gamma RII-mediated rosette formation for both situations. In contrast, supernatant of stimulated granulocytes as well as leucocyte elastase were observed to be active in augmenting EA-hIgG rosette formation of thrombocytes and myeloid cell lines K562 and U937. The B cell lines Raji and Daudi, did not show enhanced rosette formation after enzyme treatment. The active component from granulocyte supernatant was partially characterized as a serine esterase with an apparent Mw of 30 kD. We tested whether the isotype specificity of Fc gamma RII on K562 cells changes upon enzyme treatment. It was found that all three tested murine subclasses gamma 1, gamma 2a, gamma 2b, bound equally well to this receptor, and interaction with all isotypes was enhanced to the same extent.
Tuijnman
W B
WB
Department of Experimental Immunology, University of Utrecht, The Netherlands.
van Dam
F W
FW
van de Winkel
J G
JG
Capel
P J
PJ
D000943:Antigens, Differentiation / Q000378:metabolism*; D001402:B-Lymphocytes / Q000276:immunology; D001779:Blood Coagulation Factors / Q000502:physiology; D002460:Cell Line; D010450:Endopeptidases / Q000502:physiology*; D015672:Erythroid Precursor Cells / Q000276:immunology; D006801:Humans; D007132:Immunoglobulin Isotypes / Q000378:metabolism; D009000:Monocytes / Q000276:immunology; D009504:Neutrophils / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000502:physiology; D011961:Receptors, Fc / Q000378:metabolism*; D017452:Receptors, IgG; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology
D000943:Antigens, Differentiation; D001779:Blood Coagulation Factors; D007132:Immunoglobulin Isotypes; D011961:Receptors, Fc; D017452:Receptors, IgG; D010450:Endopeptidases; D010196:Pancreatic Elastase