The determination of copper in human liver biopsy specimens by cup-in-tube ETAAS.
A method for the direct determination of copper in human liver biopsy specimens is described. The addition of a mixed Mg-Pd nitrate modifier is shown effectively to delay the volatilization of copper both from aqueous standards and the powered NISTBovine Liver to an appearance time comparable with the solid biopsy specimens. Calibration against solid liver reference materials is preferable compared with aqueous copper standard solutions. A number of copper absorption lines provide a wide range of sensitivities; the less sensitive Cu-line 222.6 nm was found optimal in the determination of copper concentrations in the range of 20-200 mg/kg in liver biopsy specimens. The copper concentrations measured in liver biopsy specimens from patients suffering from sclerosing cholangitis were considerably higher than those reported for a group of healthy persons.
Aadland
E
E
Medical Department National Hospital, Oslo, Norway.
Aaseth
J
J
Dahl
K
K
Radziuk
B
B
Thomassen
Y
Y
D000818:Animals; D001706:Biopsy; D002417:Cattle; D015209:Cholangitis, Sclerosing / Q000378:metabolism*; D003300:Copper / Q000032:analysis*; D006801:Humans; D008099:Liver / Q000737:chemistry*; D011786:Quality Control; D013054:Spectrophotometry, Atomic / Q000379:methods* / Q000706:statistics & numerical data; D013552:Swine
D003300:Copper