Presence of a new microtubule cold-stabilizing factor in bull sperm dynein preparations.
Brain tubulin polymerized with dynein isolated from bull spermatozoa forms cold-stable microtubules, in contrast with microtubules made of brain tubulin polymerized by brain microtubule-associated proteins (MAPs). The level of cold-stable microtubules depends on the concentration of dynein used. Addition of dynein to cold-unstable microtubules renders these microtubules stable to cold. Although ATP and a non-hydrolysable ATP analogue increase the formation of microtubules made of tubulin and dynein, these nucleotides have no effect on dynein cold-stabilizing properties. The data suggests that a new factor, not involving the dynein ATPase active site and present in bull sperm dynein preparations, confers cold-stability to microtubules.
Eyer
J
J
Urology Research Laboratory, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada.
White
D
D
Gagnon
C
C
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D003080:Cold Temperature; D004398:Dyneins / Q000502:physiology*; D046911:Macromolecular Substances; D008297:Male; D008869:Microtubule-Associated Proteins / Q000502:physiology*; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism*; D011108:Polymers; D011485:Protein Binding; D013082:Sperm Tail / Q000648:ultrastructure*; D014638:Vanadates / Q000494:pharmacology
D046911:Macromolecular Substances; D008869:Microtubule-Associated Proteins; D011108:Polymers; D014638:Vanadates; D000255:Adenosine Triphosphate; D004398:Dyneins