Metallothionein genes from the flowering plant Mimulus guttatus.
In response to excess metal, higher plants produce metal-binding peptides [( gamma EC]nG) whose biosynthesis is believed to be mediated by enzymes involved in glutathione (gamma ECG) metabolism. In contrast, animals synthesize metallothioneins, gene-encoded low molecular weight cysteine-rich metal-binding proteins. In an investigation of copper-regulated genes in the copper-tolerant flowering plant Mimulus guttatus, we have isolated a series of cDNA clones identifying two genes which encode a protein with class I metallothionein domains. This represents the first description of a metallothionein gene in a flowering plant.
de Miranda
J R
JR
Department of Genetics and Microbiology, University of Liverpool, England.
Thomas
M A
MA
Thurman
D A
DA
Tomsett
A B
AB
D003001:Cloning, Molecular; D003300:Copper / Q000494:pharmacology; D003545:Cysteine; D004247:DNA / Q000302:isolation & purification; D004251:DNA Transposable Elements; D005786:Gene Expression Regulation / Q000187:drug effects; D008668:Metallothionein / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010944:Plants / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid
D004251:DNA Transposable Elements; D012333:RNA, Messenger; D003300:Copper; D004247:DNA; D008668:Metallothionein; D003545:Cysteine