title: Fibronectin biosynthesis: influence on fibroblast adhesion during chick embryo development.
abstract: Eight d (8d) and 16d (16d) chick embryo fibroblasts (CEF) exhibited marked differences in their adhesive capacity on plastic support, but not on fibronectin substratum. This suggests differences in fibronectin (FN) expression and/or FN receptor expression. Both 8d and 16d CEF expressed an identical number of membrane receptors for FN with similar affinity. In contrast, the newly synthesized FN appeared de novo in 30 min in 8d CEF versus 60 min in 16d CEF. This difference is not due to a modification of the polypeptide chain biosynthetic rate. The FN synthesized in 8d CEF became insensitive to endo beta-N-acetyl-glucosaminidase H (endo H) treatment after 20 min, whereas it remained sensitive to endo H until 60 min in 16d CEF. Post-translational modifications of N-linked mannose-rich chains to complex type chain may account for the difference in the expression of cell surface FN and thus for the difference in cell adhesion capacity to plastic.
authors: UFR Biomédicale des Saints-Péres, INSERM U180, Paris, France.
authors: J
authors: J
authors: Botti
authors: E
authors: E
authors: Gouet
authors: R
authors: R
authors: Moutsita
authors: M A
authors: MA
authors: Doyennette-Moyne
authors: M
authors: M
authors: Aubery
authors: P
authors: P
authors: Codogno
mesh_terms: D000818:Animals; D001665:Binding Sites; D002448:Cell Adhesion; D002462:Cell Membrane / Q000378:metabolism; D002642:Chick Embryo; D005347:Fibroblasts / Q000166:cytology*; D005353:Fibronectins / Q000096:biosynthesis*; D006596:Hexosaminidases / Q000494:pharmacology; D007700:Kinetics; D008358:Mannose / Q000378:metabolism; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D011499:Protein Processing, Post-Translational; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000378:metabolism; D013997:Time Factors
chemical_list: D005353:Fibronectins; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic; D006596:Hexosaminidases; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008358:Mannose