Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus.
A monoclonal antibody raised against mammalian spliceosomes specifically recognizes a non-snRNP factor required for spliceosome assembly. This splicing factor is highly concentrated in discrete regions within the nucleus, in a pattern that is a distinct subset of that seen with anti-snRNP antibodies. These observations are evidence that spliceosome assembly could be compartmentalized within the nucleus.
Fu
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Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
Maniatis
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D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis / Q000276:immunology; D002467:Cell Nucleus / Q000032:analysis / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D007163:Immunosorbent Techniques; D008854:Microscopy, Electron; D009687:Nuclear Proteins / Q000032:analysis* / Q000276:immunology / Q000502:physiology; D012322:RNA Precursors / Q000235:genetics; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D012261:Ribonucleoproteins / Q000032:analysis; D017411:Ribonucleoproteins, Small Nuclear
D000911:Antibodies, Monoclonal; D000941:Antigens; D009687:Nuclear Proteins; D012322:RNA Precursors; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear