title: Sparse distribution of gamma/delta T lymphocytes around human epithelial tumors predominantly infiltrated by primed/memory T cells.
abstract: Evidence from the mouse system has suggested that T lymphocytes accumulating in non-lymphoid tissue, in particular epithelia, may preferentially express the T cell receptor (TCR) gamma delta. In this study, we characterize the T cell receptor alpha beta or gamma delta phenotype of lymphocytes infiltrating human tumors of epithelial origin using monoclonal antibodies (mAb) for immunohistology and flow cytometry on cells extracted by enzyme digestion. This report shows that the majority of CD3+ tumor-infiltrating lymphocytes are TCR alpha beta+ but a small percentage of TCR gamma delta can be clearly defined scattered throughout the tumor tissue with apparently no microanatomical selection. So far there has been little evidence for an accumulation of activated T cells in human tumor tissues as defined by mAb against molecules appearing transiently during the acute phase of activation. Now mAb are available that can identify primed or memory T cells such as mAb UCHL-1 recognizing the CD45RO antigen. Here we show that CD3+ tumor-infiltrating lymphocytes have a statistically significant accumulation of primed T cells, as compared to the autologous peripheral blood lymphocytes, suggesting their immune stimulation by tumor cells.
authors: Ludwig Institute for Cancer Research, Lausanne Branch, Epalinges, Switzerland.
authors: S
authors: S
authors: Miescher
authors: M
authors: M
authors: Schreyer
authors: C
authors: C
authors: Barras
authors: P
authors: P
authors: Capasso
authors: V
authors: V
authors: von Fliedner
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000941:Antigens / Q000378:metabolism; D000943:Antigens, Differentiation / Q000378:metabolism / Q000502:physiology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism / Q000502:physiology; D017252:CD3 Complex; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000276:immunology / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006649:Histocompatibility Antigens / Q000378:metabolism / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D017493:Leukocyte Common Antigens; D008213:Lymphocyte Activation / Q000502:physiology; D016131:Lymphocyte Subsets / Q000502:physiology*; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000097:blood* / Q000276:immunology / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000502:physiology*
chemical_list: D000941:Antigens; D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D006649:Histocompatibility Antigens; D011948:Receptors, Antigen, T-Cell; D017493:Leukocyte Common Antigens