Immunogenicity of glutaraldehyde-tanned bovine pericardium.
Glutaraldehyde-tanned bovine pericardium was tested for its ability to induce immunologic responses in vivo. Sections of glutaraldehyde-tanned bovine pericardium were implanted between the abdominal muscles of rats and guinea pigs. Control animals received Dacron implants. Lymphocytes and sera from animals were isolated at 2 and 4 weeks after implantation (four animals per group per time). Tritiated thymidine incorporation and an enzyme-linked immunosorbent assay were used to measure T- and B-lymphocyte responses to glutaraldehyde-tanned bovine pericardium antigens. At the same time points, implants and surrounding tissue from all animals were processed for histologic data. Results show that T-lymphocytes from animals with glutaraldehyde-tanned bovine pericardium implants responded significantly (p less than 0.001) to glutaraldehyde-tanned bovine pericardium antigens in vitro but not to Dacron. In contrast, lymphocytes from animals with Dacron implants failed to respond to glutaraldehyde-tanned bovine pericardium or Dacron preparations. Results of enzyme-linked immunosorbent assay show that animals with glutaraldehyde-tanned bovine pericardium implants produced antibody directed against glutaraldehyde-tanned bovine pericardium antigens. Histologic study revealed a dense mononuclear and multinuclear giant cell infiltrate at the interface between glutaraldehyde-tanned bovine pericardium and surrounding host tissues, with focal degradation of implant collagen. Dacron elicited a nonspecific lymphocytic and foreign body-type reaction. These results indicate that glutaraldehyde-tanned bovine pericardium can induce immunologic responses in vivo consistent with a host-versus-graft reaction.
Dahm
M
M
Department of Cardiothoracic Surgery, Albert Einstein College of Medicine, Bronx, N.Y.
Lyman
W D
WD
Schwell
A B
AB
Factor
S M
SM
Frater
R W
RW
D000009:Abdominal Muscles / Q000601:surgery / Q000648:ultrastructure; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D001705:Bioprosthesis; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D006789:Host vs Graft Reaction / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D010496:Pericardium / Q000276:immunology / Q000648:ultrastructure; D011093:Polyethylene Terephthalates; D019736:Prostheses and Implants; D051381:Rats; D011917:Rats, Inbred Lew
D000906:Antibodies; D011093:Polyethylene Terephthalates