title: Localisation of CD10 to biliary canaliculi by immunoelectron microscopical examination.
abstract: Common acute lymphoblastic leukaemia antigen (CALLA) was first characterised in lymphoid leukaemic cells. The antigen is present in different stages of lymphoid cell differentiation as well as in subsets of myeloid cells, and further studies have also shown its presence in non-lymphoid tissues. The recent cloning and sequencing of the gene permitted deduction of its amino acid sequence which is identical with the human membrane-associated enzyme, neutral endopeptidase. Strong immunostaining for CALLA was detected in the human liver with a canalicular pattern. Immunoelectron microscopy also confirmed that the antigen was localised only in the area of the bile canaliculi. Although the function of neutral endopeptidase in the canaliculi is unknown, this antigen may prove useful in the study of biliary function and diseases.
authors: Department of Pathology, University of Hong Kong, Queen Mary Hospital.
authors: S L
authors: SL
authors: Loke
authors: C Y
authors: CY
authors: Leung
authors: K Y
authors: KY
authors: Chiu
authors: W L
authors: WL
authors: Yau
authors: K N
authors: KN
authors: Cheung
authors: L
authors: L
authors: Ma
mesh_terms: D015703:Antigens, CD / Q000032:analysis*; D000943:Antigens, Differentiation / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis*; D001648:Bile Canaliculi / Q000276:immunology* / Q000648:ultrastructure; D001653:Bile Ducts, Intrahepatic / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D008871:Microvilli / Q000276:immunology; D015260:Neprilysin
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; D000951:Antigens, Neoplasm; D015260:Neprilysin