Quality control of anti human CD3 and CD4 monoclonal antibodies.
By testing two monoclonal antibodies (MoAb), we tried out two methods, which might be suitable for quality control of anti human T cell MoAb. RIV6 and RIV9 were investigated by flow cytometry and Enzyme-linked Immunosorbent Assay (ELISA). The characteristics, specificity and biological activity of RIV6 and RIV 9 were determined and compared respectively with other CD4 specific MoAb, OKT4, OKT4a, LEU3a and the CD3 specific MoAb OKT3 and LEU4 by flow cytometry. An ELISA was set up to quantify mouse IgG3 antibody. Specificity, plate variability and day to day effects were determined. The results illustrate that ELISA and flow cytometry are valuable tools in the quality control of these antibodies.
Leerling
M F
MF
Laboratory for Control of Bacterial Vaccines, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
Vaessen
L M
LM
Reubsaet
C H
CH
Weimar
W
W
Ettekoven
H
H
Marsman
F R
FR
Kreeftenberg
J G
JG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000592:standards*; D000918:Antibody Specificity; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D017252:CD3 Complex; D015704:CD4 Antigens / Q000276:immunology*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D011786:Quality Control; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D015704:CD4 Antigens; D007074:Immunoglobulin G; D011948:Receptors, Antigen, T-Cell