Effects of vanadate on 6-phosphofructo 2-kinase activity and fructose 2,6-bisphosphate levels in cultured rat hepatocytes.
The presence of vanadate in primary cultures of rat hepatocytes produced a significant increase in the concentration of fructose 2,6-bisphosphate and in the activity of 6-phosphofructo 2-kinase. Compared with insulin, vanadate had a more potent action on the metabolite increase, but a similar effect on the 6-phosphofructo 2-kinase activity. Both the insulin- and the vanadate-dependent enhancements of 6-phosphofructo 2-kinase were inhibited by cycloheximide which specifically blocks protein synthesis on the translational level, suggesting that the increase of the enzyme activity was due to induction rather than to a change in the catalytic activity.
Miralpeix
M
M
Departament de Ciències Fisiològiques Humanes de la Nutrició, Universitat de Barcelona, Spain.
Katz
N R
NR
Bartrons
R
R
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005635:Fructosediphosphates / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D010732:Phosphofructokinase-1 / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D014638:Vanadates / Q000494:pharmacology*
D005635:Fructosediphosphates; D007328:Insulin; D014638:Vanadates; C027652:fructose 2,6-diphosphate; D010732:Phosphofructokinase-1