title: Nopaline synthase promoter is wound inducible and auxin inducible.
abstract: The activity of the nopaline synthase (nos) promoter is differentially regulated in several plant organs. In this article we demonstrate that the nos promoter is wound inducible in both vegetative and reproductive organs. The induction of the nos promoter was observed in leaves, stems, cotyledons, and various reproductive organs, suggesting that the response is not organ specific. The wound response was further enhanced by addition of auxins. Other growth substances had no effect on the wound-inducible nos promoter activity. Deletion analysis of the nos promoter indicated that the 10-base pair (GCACATACGT) Z element located between -123 and -114 or an element overlapping with this sequence is essential for the wound and auxin responses.
authors: Institute of Biological Chemistry, Washington State University, Pullman 99164-6340.
authors: G
authors: G
authors: An
authors: M A
authors: MA
authors: Costa
authors: S B
authors: SB
authors: Ha
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D004926:Escherichia coli / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects* / Q000502:physiology; D007210:Indoleacetic Acids / Q000494:pharmacology*; D008969:Molecular Sequence Data; D030821:Plants, Genetically Modified / Q000201:enzymology* / Q000235:genetics; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012231:Rhizobium / Q000201:enzymology / Q000235:genetics; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics
chemical_list: D007210:Indoleacetic Acids; D011993:Recombinant Fusion Proteins; D000594:Amino Acid Oxidoreductases; C021883:nopaline synthase