Therapeutic treatment of New Zealand mouse disease by a limited number of anti-idiotypic antibodies conjugated with neocarzinostatin.
-81 and NE-1 idiotypes (Id) of human nephritogenic anti-DNA antibodies are interspecies Id expressed also in NZB/W F1 mice. We tried to manipulate the synthesis of spontaneously occurring anti-DNA antibody using monoclonal anti-Id antibodies (D1E2 and 1F5) conjugated with a cytotoxic agent, neocarzinostatin (NCS). In vivo administration of anti-Id antibodies conjugated with NCS brought about an improvement in the survival rate of female NZB/W F1 mice. It also caused a retardation of development of lupus nephritis and decreased the numbers of anti-DNA-producing cells. The suppression of anti-DNA antibody synthesis was specific and Id-mediated. The results indicate that the use of a limited number of anti-Id antibodies in combination with a cytotoxic agent may be applicable therapeutically to autoimmune diseases.
Harata
N
N
Second Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Sasaki
T
T
Osaki
H
H
Saito
T
T
Shibata
S
S
Muryoi
T
T
Takai
O
O
Yoshinaga
K
K
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000627:therapeutic use*; D000888:Antibodies, Anti-Idiotypic / Q000627:therapeutic use*; D000974:Antibodies, Antinuclear / Q000276:immunology*; D001327:Autoimmune Diseases / Q000628:therapy*; D004277:DNA, Single-Stranded / Q000276:immunology; D007167:Immunotherapy; D000922:Immunotoxins / Q000627:therapeutic use*; D007674:Kidney Diseases / Q000473:pathology / Q000628:therapy; D007678:Kidney Glomerulus / Q000276:immunology / Q000473:pathology; D051379:Mice; D008814:Mice, Inbred NZB; D011507:Proteinuria / Q000628:therapy; D013154:Spleen / Q000276:immunology; D009353:Zinostatin / Q000627:therapeutic use*
D000903:Antibiotics, Antineoplastic; D000888:Antibodies, Anti-Idiotypic; D000974:Antibodies, Antinuclear; D004277:DNA, Single-Stranded; D000922:Immunotoxins; D009353:Zinostatin