[Effect of extracellular calcium on enhancement of norepinephrine release in hippocampus by phorbol ester].
Rabbit hippocampal slices were preloaded with [3H]norepinephrine ([3H]NE) and superfused with medium. Tetrodotoxin(TTX) inhibited [3H]NE release evoked by high K+, but did not affect on that evoked by Ca(2+)-pulse. The facilitatory effect of phorbol ester (PDB) on high K(+)-evoked [3H]NE release was elevated with increasing extracellular Ca2+ concentration. In the case of Ca(2+)-pulse-evoked [3H]NE release, the enhancement by PDB was decreased with increasing Ca2+ concentration in the extracellular space. It was suggested that the facilitation of Ca(2+)-influx was not involved in the mechanism of action of protein kinase C.
Huang
H Y
HY
Shanghai Institute of Physiology, Chinese Academy of Sciences.
Dai
S P
SP
D000818:Animals; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D005260:Female; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D009638:Norepinephrine / Q000378:metabolism*; D010703:Phorbol Esters / Q000494:pharmacology*; D011188:Potassium / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism; D011817:Rabbits; D013779:Tetrodotoxin / Q000494:pharmacology
D010703:Phorbol Esters; D013779:Tetrodotoxin; D011493:Protein Kinase C; D011188:Potassium; D002118:Calcium; D009638:Norepinephrine