Development of recombinant antigen vaccines for the control of theileriosis.
Immunization against Theileria parva involves infection with sporozoites and simultaneous treatment with a long-acting tetracycline. For T. annulata, immunization is achieved by inoculation of attenuated schizont-infected lymphocytes. The two methods are inadequate because of the use of live organisms and the methods are also bedevilled by the multiplicity of strains, particularly of T. parva. For these reasons, alternative methods of control are being sought. In this review an attempt is made to highlight advances towards subunit vaccines against T. parva and T. annulata. Several candidate antigens which are thought to induce protective responses have been identified and recombinant DNA technology is being employed to produce these antigens in bulk. Relevant antigens may be delivered as subunit vaccines by using recombinant vaccinia virus or attenuated Salmonella spp. as carriers of the genes expressing these antigens. It is likely that effective vaccines against T. parva and T. annulata will have to elaborate immune responses against both the sporozoite and schizont stages of the parasite.
Musoke
A J
AJ
International Laboratory for Research on Animal Disease, Nairobi, Kenya.
Nene
V
V
D000276:Adjuvants, Immunologic; D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis; D000953:Antigens, Protozoan / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D016782:Apicomplexa / Q000145:classification / Q000235:genetics / Q000276:immunology*; D002417:Cattle / Q000276:immunology / Q000469:parasitology; D004274:DNA, Recombinant; D013045:Species Specificity; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D013801:Theileriasis / Q000469:parasitology / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary; D014614:Vaccines, Synthetic / Q000276:immunology / Q000302:isolation & purification
D000276:Adjuvants, Immunologic; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D004274:DNA, Recombinant; D014614:Vaccines, Synthetic