Amperometric determination of phosphate by use of a nucleoside phosphorylase-xanthine oxidase enzyme sensor based on a Clark-type hydrogen peroxide or oxygen electrode.
A bienzymatic sensor for the determination of phosphate was constructed by coimmobilization of xanthine oxidase (EC 1.2.3.22) and nucleoside phosphorylase (EC 2.4.2.1) on a polycarbonate membrane mounted on the tips of amperometric hydrogen peroxide and oxygen electrodes. The sensor response was linear to phosphate concentrations in the range 10-250 microM.
Haemmerli
S D
SD
University of New Orleans, Department of Chemistry, Louisiana 70148.
Suleiman
A A
AA
Guilbault
G G
GG
D015374:Biosensing Techniques; D004566:Electrodes; D006861:Hydrogen Peroxide / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007288:Inosine / Q000378:metabolism; D009097:Multienzyme Complexes; D010101:Oxygen Consumption; D010710:Phosphates / Q000032:analysis*; D011683:Purine-Nucleoside Phosphorylase / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000378:metabolism*
D009097:Multienzyme Complexes; D010710:Phosphates; D007288:Inosine; D006861:Hydrogen Peroxide; D014969:Xanthine Oxidase; D011683:Purine-Nucleoside Phosphorylase