title: The chloroplast CF0I subunit can replace the b-subunit of the F0F1-ATPase in a mutant strain of Escherichia coli K12.
abstract: The amino acid sequence of the CF0I subunit from the chloroplast F0F1-ATPase has only a low similarity to the amino acid sequence of the b-subunit of the E. coli F0F1-ATPase. However, secondary and tertiary structure predictions plus the distribution of hydrophobic and hydrophilic amino acids have indicated that these two subunits serve a similar function. This proposition was investigated directly. A cDNA clone for the chloroplast atpF gene, encoding the CF0I subunit, was altered by site-directed mutagensis such that the translation start site corresponded to the N-terminus of the mature protein. An E. coli mutant strain carrying a chain-terminating mutation in the uncF gene, encoding the b-subunit, was transformed with the plasmid carrying the altered atpF gene. The resultant transformant was able to grow on succinate and gave a growth yield similar to that of a wild-type control. Assays on membrane preparations from the transformant also clearly indicated that the mature CF0I subunit from spinach chloroplasts was able to replace the E. coli b-subunit in the E. coli F0F1-ATPase.
authors: Division of Biochemistry and Molecular Biology, John Curtin School of Medical Research, Australian National University, Canberra City.
authors: G
authors: G
authors: Schmidt
authors: A J
authors: AJ
authors: Rodgers
authors: S M
authors: SM
authors: Howitt
authors: A L
authors: AL
authors: Munn
authors: G S
authors: GS
authors: Hudson
authors: T A
authors: TA
authors: Holten
authors: P R
authors: PR
authors: Whitfeld
authors: W
authors: W
authors: Bottomley
authors: F
authors: F
authors: Gibson
authors: G B
authors: GB
authors: Cox
mesh_terms: D000595:Amino Acid Sequence; D002736:Chloroplasts / Q000201:enzymology*; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development; D005816:Genetic Complementation Test; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D011994:Recombinant Proteins
chemical_list: D046911:Macromolecular Substances; D011994:Recombinant Proteins; D006180:Proton-Translocating ATPases