Dynamic properties of blood flow and leukocyte mobilization in infected flaps.
Two aspects of the inflammatory response to infection--blood flow alteration and leukocyte mobilization--are investigated in the canine model. The elevation of paired musculocutaneous (MC) and random pattern (RP) flaps allowed comparison of healing flaps with significant differences in blood flow (lower in random pattern flaps) and resistance to infection (greater in musculocutaneous flaps). Blood flow changes as determined by radioactive xenon washout were compared in normal skin and distal flap skin both after elevation and following bacterial inoculation. Simultaneous use of In-111 labeled leukocytes allowed determination of leukocyte mobilization and subsequent localization in response to flap infection. Blood flow significantly improved in the musculocutaneous flap in response to infection. Although total leukocyte mobilization in the random pattern flap was greater, the leukocytes in the musculocutaneous flap were localized around the site of bacterial inoculation within the dermis. Differences in the dynamic blood flow and leukocyte mobilization may, in part, explain the greater reliability of musculocutaneous flaps when transposed in the presence of infection.
Feng
L J
LJ
Department of Surgery, University of California, School of Medicine, San Francisco.
Price
D C
DC
Mathes
S J
SJ
Hohn
D
D
D000009:Abdominal Muscles / Q000098:blood supply / Q000378:metabolism / Q000473:pathology / Q000637:transplantation*; D000818:Animals; D001424:Bacterial Infections / Q000503:physiopathology*; D002465:Cell Movement; D004285:Dogs; D005260:Female; D007205:Indium Radioisotopes; D007962:Leukocytes / Q000502:physiology*; D008297:Male; D010101:Oxygen Consumption; D012039:Regional Blood Flow; D012867:Skin / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D012881:Skin Temperature; D016038:Skin Transplantation; D013524:Surgical Flaps; D014980:Xenon Radioisotopes
D007205:Indium Radioisotopes; D014980:Xenon Radioisotopes