Chicken growth-associated protein GAP-43 is tightly bound to the actin-rich neuronal membrane skeleton.
We have identified the chicken equivalent of growth-associated protein GAP-43 in a detergent-resistant membrane skeleton from cultures of chick neurones and embryonic chick brain. Antisera to the membrane skeleton protein, the 3D5 antigen, precipitate the translation product of chick GAP-43 cDNA, and the 3D5 antigen is also detected by antisera against synthetic peptides from the known amino acid sequence of rat GAP-43. The chick protein and the rat GAP-43 are biochemically similar proteins that both serve as major targets of phosphorylation by endogenous protein kinase C. The detergent-resistant complex in which GAP-43 is found also contains actin (approximately 5% of the total protein) and a neurone-specific cell surface glycoprotein. We suggest that the membrane skeleton of neurones may be a primary site of action of GAP-43.
Moss
D J
DJ
MRC Cell Biophysics Unit, King's College, London, England.
Fernyhough
P
P
Chapman
K
K
Baizer
L
L
Bray
D
D
Allsopp
T
T
D000199:Actins / Q000378:metabolism*; D000818:Animals; D002642:Chick Embryo; D002645:Chickens / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism*; D004247:DNA / Q000235:genetics; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D019922:GAP-43 Protein; D006133:Growth Substances; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D014176:Protein Biosynthesis; D011493:Protein Kinase C / Q000378:metabolism
D000199:Actins; D019922:GAP-43 Protein; D006133:Growth Substances; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D004247:DNA; D011493:Protein Kinase C