Determination of affinities of murine and chimeric anti alpha/beta-T-cell receptor antibodies by flow cytometry.
In this report we describe the use of a calibrated flow cytometer to quantify the expression of the human alpha/beta-T-cell-receptor on peripheral blood lymphocytes of healthy human blood donors and on the T-cell line HPB-ALL. To calculate the maximum of antibodies which are needed to obtain antigen saturation per cell and to determine the affinity of murine and chimeric anti-TCR antibodies, we applied Scatchard-plot and double reciprocal Klotz-plot analyses.
Krause
D
D
Research Laboratories of Behringwerke AG, Marburg, Germany.
Shearman
C
C
Lang
W
W
Kanzy
E J
EJ
Kurrle
R
R
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D002678:Chimera; D005434:Flow Cytometry / Q000379:methods; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008214:Lymphocytes / Q000276:immunology*; D046911:Macromolecular Substances; D051379:Mice; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013861:Thiocyanates
D000911:Antibodies, Monoclonal; D005452:Fluoresceins; D005456:Fluorescent Dyes; D046911:Macromolecular Substances; D011948:Receptors, Antigen, T-Cell; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate