title: Determination of megestrol acetate and cyproterone acetate in serum of patients with advanced breast cancer by high-performance liquid chromatography.
abstract: A reversed-phase high-performance liquid chromatographic method with ultraviolet detection of megestrol acetate and cyproterone acetate in human sera is described. The proposed assay is linear up to 1400 ng/ml (r = 0.999) and has a detection limit of 5 ng/ml. Recoveries of both compounds in spiked sera were ca. 95%; inter-assay coefficients of variation were 4.0 and 3.1% and intra-assay values were 1.3 and 1.4%, respectively. For validation of the method we also developed a gas chromatographic-mass spectrometric method for both steroids. The results obtained by the two methods showed good correlation: for megestrol acetate r = 0.98, n = 31, p less than 0.0001, and for cyproterone acetate r = 0.94, n = 0, p less than 0.0001. Large inter-individual differences in the serum concentrations of both substances were found in groups of patients with metastatic breast cancer receiving the same oral load of either steroid.
authors: Central Laboratory for Clinical Chemistry, University Hospital, Groningen, The Netherlands.
authors: L D
authors: LD
authors: Dikkeschei
authors: B G
authors: BG
authors: Wolthers
authors: A W
authors: AW
authors: de Ruyter-Buitenhuis
authors: G T
authors: GT
authors: Nagel
authors: D T
authors: DT
authors: Sleijfer
authors: P H
authors: PH
authors: Willemse
authors: W
authors: W
authors: van der Slik
mesh_terms: D000970:Antineoplastic Agents / Q000097:blood*; D001943:Breast Neoplasms / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D003534:Cyproterone / Q000031:analogs & derivatives* / Q000097:blood; D017373:Cyproterone Acetate; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008535:Megestrol / Q000031:analogs & derivatives* / Q000097:blood; D019290:Megestrol Acetate
chemical_list: D000970:Antineoplastic Agents; D017373:Cyproterone Acetate; D003534:Cyproterone; D008535:Megestrol; D019290:Megestrol Acetate