Sequence analysis of D-xylose isomerase gene from Escherichia coli.
Gene coding for E. coli D-xylose isomerase was cloned into the plasmid pWR13 from the plasmid pXO100 containing D-xylose operon. Several subclones harboring DNA fragments of different size obtained by step- by-step shortening with Bal31 nuclease were directly sequenced. The complete nucleotide sequence of the 1.6kb DNA fragment carrying D-xylose isomerase gene was determined. The structural gene of 1320 nucleotides codes for a protein of 440 amino acids. The additional 209 nucleotides at 5' end and 82 nucleotides at 3' end include Shine-Dalgarno ribosome-binding site and the transcriptional termination codon, respectively.
Hou
Y M
YM
Institute of Biophysic, Chinese Academy of Sciences, Beijing.
Zhang
Q J
QJ
Wang
S J
SJ
D019747:Aldose-Ketose Isomerases; D000595:Amino Acid Sequence; D001483:Base Sequence; D002238:Carbohydrate Epimerases / Q000235:genetics*; D003001:Cloning, Molecular; D003062:Codon; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012441:Saccharomyces cerevisiae / Q000235:genetics
D003062:Codon; D004269:DNA, Bacterial; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase