Adenovirus transformed monkey cell lines permissive to enteric adenovirus type 40.
Plasmids containing the E1 regions of adenovirus serotypes 3 and 5 were transfected into primary Rhesus monkey kidney cells. The presence of viral DNA sequences was detected in transformed cell lines. All these cell lines expressed the E1A proteins. In addition, Ad5 transformed cells, have the E1B 21 kDa protein located in the nuclear membrane. These cell lines were permissive to the enteric adenovirus serotype 40 but not to serotype 41.
Nascimento
J P
JP
Departamento de Virologia, Instituto Oswaldo Cruz, Rio de Janeiro, Brasil.
Leite
J P
JP
Winter
N
N
D'Halluin
J C
JC
D000256:Adenoviridae / Q000235:genetics*; D017456:Adenovirus Early Proteins; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D002472:Cell Transformation, Viral; D004279:DNA, Viral / Q000032:analysis; D005455:Fluorescent Antibody Technique; D008253:Macaca mulatta; D009856:Oncogene Proteins, Viral / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis; D014162:Transfection
D017456:Adenovirus Early Proteins; D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger