Carrier thiols are targets of Photofrin II photosensitization of isolated rat liver mitochondria.
To gain further insight into the ability of Photofrin II to photosensitize mitochondrial translocators, and to ascertain whether mitochondrial thiols are specific targets of Photofrin II, the activity of phosphate carrier was measured in isolated rat liver mitochondria irradiated with 365 nm light in the presence of Photofrin II. Photodynamic treatment decreased the maximum rate of phosphate uptake, without changing the phosphate affinity for its own carrier. The ability of the thiol reagent mersalyl (an inhibitor of phosphate, dicarboxylate and oxodicarboxylate carriers) to protect these carriers against Photofrin II photosensitization was also tested. Protection was observed, indicating the involvement of carrier thiols in mitochondrial photosensitization.
Atlante
A
A
Dipartimento di Biochimica e Biologia Molecolare, Università di Bari, Italy.
Passarella
S
S
Quagliariello
E
E
Moreno
G
G
Salet
C
C
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects / Q000528:radiation effects; D017323:Dihematoporphyrin Ether; D006415:Hematoporphyrins / Q000378:metabolism / Q000528:radiation effects*; D066298:In Vitro Techniques; D007700:Kinetics; D008027:Light; D008634:Mersalyl / Q000494:pharmacology; D008930:Mitochondria, Liver / Q000378:metabolism / Q000528:radiation effects; D010710:Phosphates / Q000378:metabolism; D010777:Photochemistry; D051381:Rats; D013438:Sulfhydryl Compounds / Q000378:metabolism*
D006415:Hematoporphyrins; D010710:Phosphates; D013438:Sulfhydryl Compounds; D008634:Mersalyl; D017323:Dihematoporphyrin Ether