Identification by sequence analysis of a second rat brain cDNA encoding the dopamine (D2) receptor.
A rat brain cDNA library constructed in lambda ZAP II was screened with three oligonucleotide probes based on the reported coding region of the D2 receptor gene, RGB-2. A complete cDNA clone, D2(8)-1, showing positive signals with the three probes was subsequently identified by restriction analysis and dideoxy sequence analysis to be a variant of the RGB-2 gene. Comparison of the two genes revealed almost complete homology except that D2(8)-1 contains an 87 bp insert within the protein coding region and 265 additional nucleotides 5' upstream from the 5' end reported for RGB-2. It is suggested that at least two mRNA species encoding for D2 receptors exist in rat brain, possibly resulting from alternative splicing of RNA.
Miller
J C
JC
Millhauser Laboratories of the Department of Psychiatry, New York University School of Medicine, New York 10016.
Wang
Y
Y
Filer
D
D
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D051381:Rats; D011954:Receptors, Dopamine / Q000235:genetics*; D017448:Receptors, Dopamine D2; D015183:Restriction Mapping
D015345:Oligonucleotide Probes; D011954:Receptors, Dopamine; D017448:Receptors, Dopamine D2; D004247:DNA