Lipopolysaccharide enhances monocyte adherence to matrix-bound fibronectin.
Fibronectin (Fn), an extracellular matrix glycoprotein with binding sites for collagen, fibrin, heparin, and cell surfaces, is a nonimmune opsonin which up-regulates phagocytic function and facilitates adherence of human monocytes. We have developed a simple assay to study adherence of peripheral blood monocytes to Fn on a gelatin matrix. While cell adherence was enhanced by the presence of Fn in a dose-dependent manner, it was inhibited by peptides containing the Arg-Gly-Asp (RGD) cell attachment sequence or by coating the matrix with antibodies directed against Fn. Preincubation of monocytes for 30 min with Escherichia coli lipopolysaccharide (LPS) at doses of 1-50 micrograms/ml increased adherence to Fn-gelatin but not to gelatin alone, while longer preincubation (24 hr) resulted in similar changes at lower doses (0.01-1.0 micrograms/ml). Enhanced Fn adherence may be essential for monocyte localization to sites of inflammation.
Roth
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Department of Pediatrics, Albert Einstein College of Medicine, Bronx, New York 10461.
Polin
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D000970:Antineoplastic Agents / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005353:Fibronectins / Q000502:physiology*; D006801:Humans; D008070:Lipopolysaccharides / Q000502:physiology*; D009000:Monocytes / Q000187:drug effects*; D009842:Oligopeptides / Q000494:pharmacology; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000187:drug effects
D000970:Antineoplastic Agents; D005353:Fibronectins; D008070:Lipopolysaccharides; D009842:Oligopeptides; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic; C049554:glycyl-arginyl-glycyl-aspartyl-serine