Multiple suppressive effects of transforming growth factor beta 1 on the immune response in chickens.
The immunosuppressive effect of human recombinant TGF-beta 1 on chicken immune responses in vitro was evaluated. TGF-beta 1 at 1-10 ng/ml reduced T cell proliferation in response to concanavalin A by 50-80% and B cell proliferation in response to LPS by greater than 90%. In contrast, when added to immune spleen cells, it reduced the secondary PFC response to sheep erythrocytes by less than 50%, particularly when added at the same time as antigen on Day 2 of incubation. When TGF-beta 1 was added during a 2-day incubation to nylon wool-nonadherent immune or normal spleen cells, it caused the maintenance and/or appearance of suppressor cells. These suppressor cells, in coculture with immune spleen cells, inhibited the secondary PFC response in vitro without any further exposure to TGF-beta 1. The phenotype of the cells giving rise to suppressor cells under the influence of TGF-beta 1 was CT8+, TCR2+(alpha,beta), CT4-, TCR1-(gamma,delta) cells. The results suggest that, in addition to direct suppressive effects on the proliferation of B cells and of some T cells, TGF-beta 1 may suppress immune responses by maintaining or by promoting the development of suppressor T cells.
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Station de Pathologie Aviaire, Institut National de la Recherche Agronomique, Monnaie, France.
Thorbecke
G J
GJ
D000818:Animals; D000917:Antibody Formation / Q000502:physiology*; D001402:B-Lymphocytes / Q000502:physiology; D002455:Cell Division / Q000276:immunology; D002645:Chickens / Q000276:immunology; D006462:Hemolytic Plaque Technique; D007108:Immune Tolerance; D066298:In Vitro Techniques; D010641:Phenotype; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000502:physiology; D050378:T-Lymphocytes, Regulatory / Q000502:physiology*; D015291:Transforming Growth Factors / Q000502:physiology*
D015291:Transforming Growth Factors