title: Effect of soluble and immobilized plasmin on fibrinogen and platelets.
abstract: Plasmin was immobilized on collageno-elastic tubes (CET) using carbodiimide as the cross-linking agent. The effects of plasmin-CET grafts and corresponding soluble plasmin on fibrinogen, thrombin-mediated fibrinogen activation, and platelet activity, were investigated. There was a significant increase in fibrinogen deposition on plasmin-CETs over non-plasmin (i.e. control) CETs. Furthermore, exposure of fibrinogen to plasmin CETs enhances its deposition to control grafts situated downstream. Plasmin-bound CETs retained higher platelet deposition when preliminarily coated with fibrinogen. Finally, plasmin exerted a positive effect on thrombin-mediated fibrinogen activation at low plasmin concentrations. A mechanistic hypothesis aimed at interpreting this finding is proposed.
authors: Department of Chemical Engineering, Texas Tech University, Lubbock 79409.
authors: F
authors: F
authors: Senatore
authors: H
authors: H
authors: Shankar
authors: C H
authors: CH
authors: Ho
authors: S
authors: S
authors: Avantsa
authors: M
authors: M
authors: Allon
authors: R
authors: R
authors: Posteraro
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000187:drug effects*; D003094:Collagen; D004800:Enzymes, Immobilized / Q000494:pharmacology*; D005340:Fibrinogen / Q000187:drug effects*; D005341:Fibrinolysin / Q000494:pharmacology*; D011817:Rabbits; D012995:Solubility
chemical_list: D004800:Enzymes, Immobilized; D005340:Fibrinogen; D003094:Collagen; D005341:Fibrinolysin