The inhibitory action of oxygen radical scavengers on proteinuria and glomerular heparan sulphate loss in the isolated perfused kidney.
The perfused isolated kidney is a partial ischemic system that is characterised by glomerular proteinuria and release of glomerular heparan sulfate. Metabolic changes associated with the levels of glutathione, xanthine oxidase and glyceraldehyde 3-dehydrogenase indicated that oxygen radical metabolites were being produced during the perfusion. We have demonstrated that a mixture of oxygen metabolite scavengers containing mannitol, superoxide dismutase and catalase included in the perfusion medium significantly reduced protein excretion. Similar results were obtained with the administration of allopurinol to the rat 24h prior to kidney removal and allopurinol in the perfusion medium. [35S]Heparan sulfate loss from the glomerulus was totally inhibited by the scavenger mixture. These results suggest that reactive oxygen metabolites may be involved in damage to renal capillaries, specifically to heparan sulfate proteoglycan, which leads to proteinuria as a result of partial ischemia produced during perfusion.
Tay
M
M
Biochemistry Department, Monash University, Clayton, Victoria, Australia.
Comper
W D
WD
Vassiliou
P
P
Glasgow
E F
EF
Baker
M S
MS
Pratt
L
L
D000818:Animals; D002374:Catalase / Q000494:pharmacology; D005609:Free Radicals; D005978:Glutathione / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism*; D006497:Heparan Sulfate / Q000378:metabolism*; D007678:Kidney Glomerulus / Q000378:metabolism*; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism*; D010477:Perfusion; D011507:Proteinuria / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013482:Superoxide Dismutase / Q000494:pharmacology; D014969:Xanthine Oxidase / Q000378:metabolism
D002374:Catalase; D005609:Free Radicals; D005978:Glutathione; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D008353:Mannitol; D010100:Oxygen; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase