title: [Purification and various properties of Vibrio cholerae NON-01 cytolysin].
abstract: A new method for isolation and purification of Vibrio cholerae non-01 cytolysin has been elaborated. It includes the steps of concentration by ammonium sulphate, ion exchange chromatography on DE-52-cellulose, gel filtration via ultragel AsA-44 and chromatography on Mono Q. Cytolysin is shown to be a 60 kD and pI 6.2 protein. It is not inactivated by thiol-restoring agents and induces the production of precipitating antibodies. In contrast to choleragen the protein cytolytically affects erythrocytes and cells of the line L-929. It also possesses the lethal and oedematous effect and demonstrates the enteropathogenic effect on nursing rabbits.
authors: A O
authors: AO
authors: Tsittser
authors: Iu V
authors: IuV
authors: Ezepchuk
authors: N O
authors: NO
authors: Nakisbekov
authors: A V
authors: AV
authors: Li
authors: V L
authors: VL
authors: Semiotrochev
authors: Iu L
authors: IuL
authors: Kiselev
mesh_terms: D000818:Animals; D002852:Chromatography, Ion Exchange; D003603:Cytotoxins / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D007928:Lethal Dose 50; D051379:Mice; D011817:Rabbits; D014734:Vibrio cholerae / Q000378:metabolism*
chemical_list: D003603:Cytotoxins
vernacular_title: Ochistka i nekotorye svoĭstva tsitolizina Vibrio cholerae NON-01.