Translational efficiency of phi X174 lysis gene E is unaffected by upstream translation of the overlapping gene D reading frame.
The lysis gene E of bacteriophage phi X174 is entirely embedded in gene D. Expression studies of genes D and E in Escherichia coli minicells and lysis times obtained in the presence or absence of D translation showed that the simultaneous expression of gene D does not affect protein E production. Thus, unlike other overlapping gene pairs, gene E expression is independent from the upstream translation of gene D. lacZ fusion studies and primer extension inhibition analysis (toeprinting) revealed an intrinsically weak E ribosome-binding site, which seems to be the major factor determining the low expression rate of the gene and thus proper scheduling of cell lysis.
Bläsi
U
U
Department of Biochemistry and Biophysics, Texas A&M University, College Station 77843.
Nam
K
K
Lubitz
W
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Young
R
R
D000595:Amino Acid Sequence; D010584:Bacteriophage phi X 174 / Q000235:genetics*; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005814:Genes, Viral; D008242:Lysogeny; D008969:Molecular Sequence Data; D010641:Phenotype; D010957:Plasmids; D014176:Protein Biosynthesis; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; D014764:Viral Proteins