Immobilization of alpha-human atrial natriuretic peptide on insoluble supports and affinity purification of specific antibodies from a polyclonal goat anti-alpha-human atrial natriuretic peptide serum.
alpha-Human atrial natriuretic peptide (alpha-hANP) was covalently coupled via single attachment onto two different insoluble matrices. Controlled-pore glass-alpha-hANP matrices were well suited for the purification of monospecific antibodies, whereas Enzacryl AA-alpha-hANP did not withstand the inevitable chemical and physical stresses during affinity purification.
Missbichler
A
A
II. Medizinische Universitäts Klinik, Vienna, Austria.
Ferris
R
R
Hartter
E
E
Woloszczuk
W
W
Pittner
F
F
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000918:Antibody Specificity; D009320:Atrial Natriuretic Factor / Q000276:immunology / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D006041:Goats / Q000276:immunology; D006801:Humans; D011863:Radioimmunoassay
D000906:Antibodies; D009320:Atrial Natriuretic Factor