Serine esterases: structural conservation during animal evolution and variability in enzymatic properties in the genus Drosophila.
Both general esterases and acetylcholinesterases have been shown to be members of a homologous superfamily of serine esterases. A comparison of N-terminal sequences demonstrates that esterase-4 and -5 from Drosophila mojavensis belong to this family as well, with esterase-6 and esterase-P from D. melanogaster being the closest relatives. In order to investigate the presence of immunologically related esterases in other Drosophila species, crude larval extracts from five species were applied to two immunoaffinity columns with antibodies directed against esterase-4 and esterase-5 from D. mojavensis. The substrate preference for either 1- or 2-naphthyl acetate was determined. Both esterase-4 and esterase-5 from D. mojavensis are 'normally' specific for 2-naphthyl esters, but at least three of the cross-reacting esterases from the other species have a preference for 1-naphthyl esters. This difference in substrate preference is another example of the variability observed with Drosophila esterases.
Pen
J
J
Department of Medical Microbiology, Rijksuniversiteit Groningen, The Netherlands.
Bolks
G J
GJ
Hoeksema-Du Pui
M L
ML
Beintema
J J
JJ
D000595:Amino Acid Sequence; D000818:Animals; D005075:Biological Evolution; D001921:Brain / Q000201:enzymology; D002846:Chromatography, Affinity; D004330:Drosophila / Q000201:enzymology*; D004331:Drosophila melanogaster / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D004950:Esterases / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D011817:Rabbits; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013379:Substrate Specificity; D014101:Torpedo
D004950:Esterases; C022635:serine esterase