Comparison of different methods for iodothyronines extraction from animal tissues.
Four different methods for thyroid hormones concentration in various tissues have been compared. The methods involve homogenization, extraction with 95% ethanol and assay of iodothyronines by a highly sensitive RIA. The method described by Chopra et al. has been selected and used for further estimations as the most reliable and convenient for a continuous laboratory work. The T4 levels (means +/- SE) found in the liver, kidney and skeletal muscle were 22.49 +/- 2.19, 14.89 +/- 1.56 and 8.33 +/- 1.73 ng per g wet tissue. The T3 contents were 8.53 +/- 1.08, 9.52 +/- 0.69 and 4.90 +/- 0.76 ng per g wet tissue, respectively.
Krysin
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Department of Developmental Endocrinology Polish Academy of Sciences, Poznań.
D000704:Analysis of Variance; D000818:Animals; D002498:Centrifugation; D000431:Ethanol / Q000494:pharmacology; D007668:Kidney / Q000737:chemistry*; D008099:Liver / Q000737:chemistry*; D008297:Male; D009132:Muscles / Q000737:chemistry*; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D013974:Thyroxine / Q000737:chemistry / Q000302:isolation & purification*; D014284:Triiodothyronine / Q000737:chemistry / Q000302:isolation & purification*
D014284:Triiodothyronine; D000431:Ethanol; D013974:Thyroxine