Microinjection of a conserved peptide sequence of p34cdc2 induces a Ca2+ transient in oocytes.
The product of the yeast cell cycle control gene cdc2, and its homologs in higher eukaryotes (p34cdc2), all contain a perfectly conserved sequence of 16 amino acids that has not been found in any other protein sequence. Microinjection of this peptide triggers a specific increase in the concentration of intracellular free Ca2+ that originates from intracellular stores in both starfish and Xenopus oocytes. Thus, p34cdc2 might interact through its conserved peptide domain with some component of the Ca2(+)-regulatory system.
Picard
A
A
CNRS and INSERM, Montpellier, France.
Cavadore
J C
JC
Lory
P
P
Bernengo
J C
JC
Ojeda
C
C
Dorée
M
M
D000595:Amino Acid Sequence; D000818:Animals; D016203:CDC2 Protein Kinase; D002118:Calcium / Q000378:metabolism*; D018118:Chloride Channels; D002712:Chlorides / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000502:physiology; D004533:Egtazic Acid / Q000494:pharmacology; D005089:Exocytosis / Q000187:drug effects; D005260:Female; D005800:Genes, Fungal; D006133:Growth Substances / Q000235:genetics*; D016200:Maturation-Promoting Factor; D008565:Membrane Proteins / Q000378:metabolism; D008845:Microinjections; D008969:Molecular Sequence Data; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D010446:Peptide Fragments; D010455:Peptides / Q000494:pharmacology*; D013215:Starfish; D014981:Xenopus
D018118:Chloride Channels; D002712:Chlorides; D006133:Growth Substances; D008565:Membrane Proteins; D010446:Peptide Fragments; D010455:Peptides; C062418:PSTAIR peptide; D004533:Egtazic Acid; D016203:CDC2 Protein Kinase; D016200:Maturation-Promoting Factor; D002118:Calcium