Molecular cloning of a cDNA encoding aldosterone synthase cytochrome P-450 in rat adrenal cortex.
Using an oligonucleotide probe designed on the basis of the N-terminal amino acid sequence of purified rat aldosterone synthase cytochrome P-450 [(1989) J. Biol. Chem. 264, 10935] we have isolated from rat adrenal cDNA library a 2687 base pair cDNA that encodes a protein of 500 amino acid residues. The deduced amino acid sequence contained the regions well conserved among all cytochrome P-450s sequenced to date, and also a portion (residues 25-44) which was identical to the N-terminal peptide sequence of rat aldosterone synthase cytochrome P-450. These results indicate that the cDNA encodes a precursor form of rat aldosterone synthase cytochrome P-450.
Imai
M
M
Department of Biochemistry, School of Medicine, Keio University, Tokyo, Japan.
Shimada
H
H
Okada
Y
Y
Matsushima-Hibiya
Y
Y
Ogishima
T
T
Ishimura
Y
Y
D000302:Adrenal Cortex / Q000201:enzymology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D019405:Cytochrome P-450 CYP11B2; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D051381:Rats; D015183:Restriction Mapping; D013250:Steroid Hydroxylases / Q000235:genetics*
D015345:Oligonucleotide Probes; D004247:DNA; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D019405:Cytochrome P-450 CYP11B2