Enhanced protection of cyclophosphamide-treated mice against infection with Pseudomonas aeruginosa after treatment with Z-100, a polysaccharide-rich extract from Mycobacterium tuberculosis Aoyama B.
We investigated the effect of Z-100, a polysaccharide-rich extract from Mycobacterium tuberculosis strain Aoyama B, on the protection of immunocompromised mice against infection with Pseudomonas aeruginosa. Mice were given cyclophosphamide and then treated with varying doses of Z-100 for 5 days or left untreated. One day after the completion of the treatment, they were challenged with P. aeruginosa and the mortality was scored. A significantly enhanced protection was observed in the treated group and bacterial number in organs was significantly lower compared with non-treated group. Though the activity of phagocytes did not appear to be activated since there was no difference in chemiluminescence response of peritoneal phagocytes between control and Z-100-treated groups, an enhanced accumulation of phagocytes was observed after bacterial challenge in Z-100-treated group. It was suggested that Z-100 is effective in the restoration of impaired resistance of immuno-compromised mice to bacterial infection possibly through a stimulation of phagocyte accumulation into the site of infection.
Kawamura
I
I
Department of Bacteriology, Niigata University School of Medicine, Japan.
Mitsuyama
M
M
Nomoto
K
K
D000276:Adjuvants, Immunologic; D000818:Animals; D015169:Colony Count, Microbial; D003520:Cyclophosphamide / Q000633:toxicity; D005260:Female; D008055:Lipids / Q000008:administration & dosage / Q000494:pharmacology*; D008163:Luminescent Measurements; D008351:Mannans / Q000008:administration & dosage / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000276:immunology; D010529:Peritoneal Cavity / Q000166:cytology; D010586:Phagocytes / Q000276:immunology; D011552:Pseudomonas Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*
D000276:Adjuvants, Immunologic; D008055:Lipids; D008351:Mannans; C031208:specific substance maruyama; D003520:Cyclophosphamide