Correlation between the lack of mononuclear cell cytotoxicity against schistosomula in vitro and inositolphosphate turnover in patients with schistosomiasis.
A comparison was made of the reactivity of mononuclear cells from normal subjects and from S. mansoni-infected patients. The following parameters were evaluated: 1) ability of mononuclear cells to kill schistosoma in the presence of complement; 2) [3H]-inositol incorporation into phosphatidylinositol (PI) and the rate of inositolphosphates (IPx) released. Cells from normal subjects, but not from S. mansoni-infected patients, were able to kill schistosomula in vitro. A decrease in inositolpolyphosphates (IPx) was observed for phytohemagglutinin (PHA)-stimulated mononuclear cells from infected patients when compared with mononuclear cells from normal subjects after 24 h of incubation. The results suggest that the reactivity of mononuclear cells from infected patients is altered under conditions of nonspecific stimulation with PHA when compared with normal cells.
Chaves
M M
MM
Departamento de Bioquímica e Imunologia, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil.
Lima-e-Silva
F C
FC
Gomez
M V
MV
Nogueira-Machado
J A
JA
D000818:Animals; D003601:Cytotoxicity Tests, Immunologic; D006801:Humans; D066298:In Vitro Techniques; D007295:Inositol Phosphates / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D012550:Schistosoma mansoni / Q000276:immunology; D012555:Schistosomiasis mansoni / Q000276:immunology* / Q000378:metabolism
D007295:Inositol Phosphates; D010835:Phytohemagglutinins