Intraepithelial TcR alpha/beta+ lymphocytes express CD45RO more often than the TcR gamma/delta+ counterparts in coeliac disease.
Expression of CD45RO on intraepithelial lymphocytes (IEL) bearing the T-cell receptor (TcR) alpha/beta or gamma/delta was studied in situ by three-colour immunofluorescence on jejunal tissue sections from 21 patients with coeliac disease and eight controls. CD45RA-TcR alpha/beta+ IEL expressed CD45RO significantly more often (75%) than the preferentially expanded TcR gamma/delta+ counterpart (59%). Triple staining for CD3, CD4/8 and CD45RA or CD45RB revealed that all CD3 + 4 - 8 - IEL (taken to be TcR gamma/delta+) expressed CD45RB and none were CD45RA. CD45RO positivity was of the same magnitude (66%) on the predominating monoclonal antibody delta TCS1-reactive fraction of TcR gamma/delta+ cells as on the remainder of the TcR gamma/delta+ subset. These results suggest that gluten exposition in patients with coeliac disease leads to accumulation of CD45RA-, putative antigen-primed memory cells of both TcR phenotypes. The less marked CD45RO expression within the preferentially expanded TcR gamma/delta+ subset of IEL may be of particular biological interest.
Halstensen
T S
TS
Institute of Pathology, University of Oslo, National Hospital, Norway.
Farstad
I N
IN
Scott
H
H
Fausa
O
O
Brandtzaeg
P
P
D000293:Adolescent; D000328:Adult; D000368:Aged; D000943:Antigens, Differentiation / Q000032:analysis*; D002446:Celiac Disease / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004848:Epithelium / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006649:Histocompatibility Antigens / Q000032:analysis*; D006801:Humans; D007223:Infant; D007413:Intestinal Mucosa / Q000276:immunology; D007583:Jejunum / Q000276:immunology*; D017493:Leukocyte Common Antigens; D008875:Middle Aged; D011948:Receptors, Antigen, T-Cell / Q000032:analysis*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000943:Antigens, Differentiation; D006649:Histocompatibility Antigens; D011948:Receptors, Antigen, T-Cell; D017493:Leukocyte Common Antigens