Differential expression of laminin chains and receptor (LBP-32) in fetal and neoplastic hepatocytes compared to normal adult hepatocytes in vivo and in culture.
Laminin deposition is increased in fetal liver and in a variety of liver diseases, including the development of carcinoma. We investigated the role of the hepatocyte in the synthesis of both laminin and its 32- to 67-kd receptor in normal adult, fetal, and diethylnitrosamine-treated rat livers, and in adult rat hepatocyte primary cultures. Laminin was localized by immunoelectron microscopy in the endoplasmic reticulum of hepatocytes in fetal-derived and in 18-month-old diethylnitrosamine-treated rat livers. Steady-state mRNA levels for the three chains of laminin (A, B1, and B2) and the laminin receptor (LBP-32) were examined. Northern-blot analyses showed that hepatocytes at all stages lacked the A-chain mRNA. B1-chain mRNA was undetectable in normal adult hepatocytes, while significant levels of B1-chain mRNA were found in fetal hepatocytes and in adult hepatocyte primary culture. In hepatocytes from diethylnitrosamine-treated rats, B1-chain mRNAs were abundant and were present mainly in nodular formations rather than in the nontumorous areas. B2-chain mRNAs were barely detectable in either normal adult or fetal hepatocytes. In diethylnitrosamine-treated rats, the steady-state B2-chain mRNA level was higher in nodules than in nontumorous areas. In primary culture, B2-chain mRNAs were present as early as 4 hours after adult hepatocyte seeding, and dramatically increased during the following 2 days. Only low levels of laminin-receptor (LBP-32) mRNAs were present in normal adult hepatocytes, whereas the levels were high in the fetal and in the tumor-containing livers. In diethylnitrosamine-treated rats, LBP-32 mRNAs were more abundant in nodular formations rather than in nontumorous areas. In hepatocyte primary culture, the expression of the LBP-32 mRNA dramatically increased during the first 24 hours. These results show that in hepatocytes, expression of laminin chains and its receptor LBP-32 are not coordinated and depends on the maturation of the cells. In addition, they suggest that the expression of B1 and B2 chains in adult hepatocytes is related to changes of the normal phenotype and/or the pericellular environment.
Rescan
P Y
PY
Hepatology Research Unit, Pontchaillou Hospital, Rennes, France.
Clément
B
B
Yamada
Y
Y
Segui-Real
B
B
Baffet
G
G
Guguen-Guillouzo
C
C
Guillouzo
A
A
D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D005260:Female; D005333:Fetus; D007124:Immunoenzyme Techniques; D007797:Laminin / Q000032:analysis* / Q000235:genetics; D008099:Liver / Q000473:pathology* / Q000648:ultrastructure; D008114:Liver Neoplasms, Experimental / Q000235:genetics / Q000473:pathology*; D008854:Microscopy, Electron; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D011971:Receptors, Immunologic / Q000032:analysis* / Q000235:genetics; D017474:Receptors, Laminin; D014407:Tumor Cells, Cultured
D007797:Laminin; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D017474:Receptors, Laminin