A murine macrophage line of the H-2d/f haplotype can activate H-2k suppressor T cells.
Little is known about the molecular basis for activation of suppressor T cells. In this report we describe two macrophage cell lines, BAC1.2.SC8 and its variant progeny B26, that differ in their ability to activate suppressor T cells. The SC8 line is derived from a (BALB/c x A.CA)F1 (H-2d/f) mouse and is haploid with respect to I-Ed. It is capable of activating I-Ed-restricted helper T cells as well as poly-(Glu50Tyr50)-specific I-Ed-restricted suppressor cells. The B26 variant can activate H-2d-restricted helper T cells but activates H-2k-restricted suppressor cells. The I-Ed molecules of SC8 and of B26 have identical amino acid sequences. This suggests that suppressor T cells either recognize posttranslational modifications of the I-E molecule or that there is another accessory molecule that helps determine the major histocompatibility complex restriction in the activation of suppressor T cells.
Chang
M D
MD
Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461.
Jaureguiberry
B
B
Garrido
E
E
Diamond
B
B
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D001483:Base Sequence; D002460:Cell Line; D006183:H-2 Antigens / Q000235:genetics*; D006239:Haplotypes; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology*; D008285:Major Histocompatibility Complex; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D016133:Polymerase Chain Reaction; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II; C024138:I-E-antigen