Interleukin-2 driven nuclear translocation of prolactin in cloned T-lymphocytes.
The nuclear translocation of PRL is demonstrated at the immunofluorescence and electron microscopic (EM) levels in interleukin-2 (IL2)-stimulated cloned T-cells and concanavalin A-stimulated splenocytes. This translocation occurs 2-10 h after IL2 stimulation, and is reversed by the addition of anti-PRL antiserum into the extracellular culture medium. The nuclear localization of PRL in IL2 stimulated T-cells was confirmed by postembedding immunogold EM. The nuclear uptake of PRL after IL2 stimulation was further documented by EM studies using PRL-colloidal gold conjugates. These studies suggest that the intranuclear PRL is translocated from the extracellular medium via an endosomal/lysosomal pathway over a period of several hours. Finally, the requirement for PRL no later than 6 h after IL2 stimulation is demonstrated through the reversible inhibition of T-cell growth with anti-PRL antiserum.
Clevenger
C V
CV
Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia 19104.
Sillman
A L
AL
Prystowsky
M B
MB
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D002999:Clone Cells; D005455:Fluorescent Antibody Technique; D007376:Interleukin-2 / Q000494:pharmacology*; D051379:Mice; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D011388:Prolactin / Q000378:metabolism*; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
D007376:Interleukin-2; D011388:Prolactin