Identification of an intermediate form of beta-N-acetylhexosaminidase in chorionic villi.
A minor form of beta-N-acetylhexosaminidase has been found in chorionic villi, in addition to the major forms A and B. This form does not hydrolyze the 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulpha te substrate, is thermostable, has a higher mol mass (120,000) than A and B (100,000) and on analytical isoelectric focusing, it shows a microheterogeneity with values ranging between 6.3 and 7.0. For these characteristics, it resembles beta-N-Acetylhexosaminidase P from pregnancy serum, from which is chromatographically indistinguishable.
Pieretti
M
M
Istituto Ginecologia ed Ostetricia, University of Perugia, Italy.
Beccari
T
T
Cosmi
E V
EV
Stirling
J L
JL
Orlacchio
A
A
D000838:Anions; D002824:Chorionic Villi / Q000201:enzymology*; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D004795:Enzyme Stability; D005260:Female; D006358:Hot Temperature; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000302:isolation & purification*; D007700:Kinetics; D008970:Molecular Weight; D011233:Precipitin Tests; D011247:Pregnancy; D001619:beta-N-Acetylhexosaminidases / Q000302:isolation & purification*
D000838:Anions; D007527:Isoenzymes; D001619:beta-N-Acetylhexosaminidases