[Hepatic proliferation factor of human fetus liver cells].
It was demonstrated in this study that after ultrasonification and centrifugation of human fetus liver cells the supernatant could increase the incorporation of 3H-TdR into DNA of mice liver cells approximately 2.57 times than that of control. This proliferation effect is not species-specific, but has a relationship with supernatant content. The maximal promotion of 3H-TdR into DNA of the guinea-pig liver cells took place when the supernatant protein content was about 0.55 mg/ml. The supernatant can be divided into four components by Ultrogel A6 chromatography and the fourth component is the dominant both in protein content and in proliferation activity. SDS-polyacrylamide gel electrophoresis showed that its molecular weight is about 15,000 dalton.
Xie
M
M
Institute of Hepatology, Beijing Army General Hospital.
Gu
F
F
Wang
Y N
YN
D000818:Animals; D001798:Blood Proteins / Q000502:physiology*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D005333:Fetus; D006168:Guinea Pigs; D017228:Hepatocyte Growth Factor; D006801:Humans; D008099:Liver / Q000737:chemistry / Q000166:cytology*; D008115:Liver Regeneration; D008297:Male; D051379:Mice
D001798:Blood Proteins; D017228:Hepatocyte Growth Factor; D004247:DNA