[Assay of sulfhydryl groups in cardiac myofibrillar proteins: effect of oxygen radicals in vitro].
Myofibrils from rat hearts were prepared in conditions maintaining their redox state, and their sulfhydryl groups were measured using a solution of urea and sodium dodecyl sulfate (SDS) as denaturant. The sulfhydryl content was 92 n mol/mg of protein, indicating that cysteins are in reduced form. In the presence of superoxide radicals generated in vitro with purine and xanthine oxidase, the myofibrillar sulfhydryl groups were oxidized.
Berson
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G
Unité de Recherche de Pharmacologie Cardiovasculaire, INSERM U-195, Faculté de Médecine, Clermont-Ferrand.
Marquet
A
A
D000818:Animals; D005609:Free Radicals; D066298:In Vitro Techniques; D008297:Male; D009124:Muscle Proteins / Q000737:chemistry*; D009206:Myocardium / Q000737:chemistry*; D009210:Myofibrils / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry / Q000494:pharmacology*; D011687:Purines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013438:Sulfhydryl Compounds / Q000032:analysis*; D014969:Xanthine Oxidase / Q000494:pharmacology
D005609:Free Radicals; D009124:Muscle Proteins; D011687:Purines; D013438:Sulfhydryl Compounds; D014969:Xanthine Oxidase; D010100:Oxygen
Dosage des groupes sulfhydryles des protéines myofibrillaires cardiaques: effet des radicaux libres oxygénés in vitro.