nit-2, the major positive-acting nitrogen regulatory gene of Neurospora crassa, encodes a sequence-specific DNA-binding protein.
The nit-2 major nitrogen regulatory gene of Neurospora crassa turns on the expression of various unlinked structural genes that specify nitrogen-catabolic enzymes under nitrogen-limitation conditions. The nit-2 gene encodes a protein of 1036 amino acid residues with a single zinc finger and a downstream basic region that may make up a DNA-binding domain. The zinc-finger domain of the NIT2 protein was synthesized in two ways to examine its DNA-binding activity with gel-band-mobility shift and DNA-footprint experiments. The NIT2 protein binds to specific DNA recognition elements that are located upstream of nitrogen-regulated structural genes. Each recognition element contains at least two copies of a core sequence whose consensus is TATCTA.
Fu
Y H
YH
Department of Biochemistry, Ohio State University, Columbus 43210.
Marzluf
G A
GA
D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D005800:Genes, Fungal; D008667:Metalloproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000235:genetics* / Q000378:metabolism; D009586:Nitrogen Fixation / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D004268:DNA-Binding Proteins; D008667:Metalloproteins; D011993:Recombinant Fusion Proteins