Identification of a gene necessary for cell cycle arrest by a negative growth factor of yeast: FAR1 is an inhibitor of a G1 cyclin, CLN2.
alpha factor is a negative growth factor and differentiation factor that induces G1 arrest and increases transcription of mating genes in S. cerevisiae a cells. We have identified a gene, FAR1 (for "factor arrest"), which is necessary for cell cycle arrest but not for other responses to alpha factor: far1- mutants are insensitive to arrest despite having an intact signal transduction pathway. FAR1 is a nonessential gene whose expression is induced 4- to 5-fold in a cells by alpha factor. The sequence of FAR1 indicates no significant similarities to known proteins. A null mutation in the CLN2 gene, which codes for a G1 cyclin, reverses the effect of a far1 null mutation: far1- cln2- strains arrest in response to alpha factor. We thus propose that FAR1 contributes to cell cycle arrest by inhibiting CLN2. The behavior of far1- cln2- strains indicates that products other than FAR1 are responsible for inhibiting the other G1 cyclins, CLN1 and CLN3.
Chang
F
F
Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448.
Herskowitz
I
I
D000595:Amino Acid Sequence; D001483:Base Sequence; D002453:Cell Cycle; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D016213:Cyclins / Q000096:biosynthesis / Q000235:genetics*; D005800:Genes, Fungal; D016153:Genes, Suppressor; D005838:Genotype; D007399:Interphase; D000072235:Mating Factor; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010455:Peptides / Q000502:physiology; D010675:Pheromones; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development
D016213:Cyclins; D010455:Peptides; D010675:Pheromones; D011993:Recombinant Fusion Proteins; D000072235:Mating Factor