Structural effects on the interaction of sterols with the (Ca2+ + Mg2+)-ATPase.
The fluorescence quenching properties of a brominated derivative of androstenol 5 alpha,6 beta-dibromoandrostan-3 beta-ol have been used to study binding to phospholipid bilayers and to the (Ca2+ + Mg2+)-ATPase purified from sarcoplasmic reticulum of rabbit skeletal muscle. It is shown that androstenol is excluded from the phospholipid/protein interface of the ATPase but can bind to other (non-annular sites) on the ATPase. Binding to these sites increases in strength with decreasing chain length for the phospholipids present in the system. Binding is also stronger in the presence of phospholipids in the gel phase than in the liquid crystalline phase. Androstenol increases the ATPase activity of the ATPase reconstituted with phosphatidylcholines of chain lengths less than C18, but has no effect on activity for the ATPase reconstituted with phosphatidylcholines of chain lengths C18 or greater. The effects of cholestanols on the activity of the ATPase reconstituted with dimyristoleoylphosphatidylcholine depend on the configuration of the sterol, with 5 alpha-cholestan-3 alpha-ol having little effect but the other isomers causing a marked stimulation.
Michelangeli
F
F
Department of Biochemistry, University of Southampton, U.K.
East
J M
JM
Lee
A G
AG
D000737:Androstenols / Q000494:pharmacology; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D005453:Fluorescence; D007700:Kinetics; D008051:Lipid Bilayers / Q000378:metabolism*; D009132:Muscles / Q000187:drug effects / Q000201:enzymology*; D010743:Phospholipids / Q000378:metabolism; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000201:enzymology*; D013261:Sterols / Q000494:pharmacology*; D013329:Structure-Activity Relationship
D000737:Androstenols; D008051:Lipid Bilayers; D010743:Phospholipids; D013261:Sterols; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases