Role of plasmin in the degradation of the stroma-derived fibrin in human ovarian carcinoma.
The aim of this study was to evaluate the type of enzymes involved in tumor-associated fibrinolysis of the stroma component fibrin in ovarian cancer patients. For this purpose, the high-molecular-mass fibrin degradation products (HMM-XDP) were isolated from malignant ascitic fluid by protamine sulfate precipitation and further purified by gel filtration and acid precipitation. After reduction with 2-mercaptoethanol, the peptide chain components were separated by reverse-phase high-performance liquid chromatography (RP-HPLC). The nature of these components was elucidated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and N-terminal amino acid sequence analysis and compared with fibrin-derived fragments formed in vitro. The results indicate that plasmin is the essential protease involved in the degradation of the stroma-derived fibrin portion found in ovarian cancer ascites.
Wilhelm
O
O
Frauenklinik der Technischen Universität München, FRG.
Hafter
R
R
Henschen
A
A
Schmitt
M
M
Graeff
H
H
D000595:Amino Acid Sequence; D001202:Ascitic Fluid / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003536:Cystadenocarcinoma / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005176:Factor XIII / Q000494:pharmacology; D005260:Female; D005337:Fibrin / Q000302:isolation & purification / Q000378:metabolism*; D005340:Fibrinogen / Q000378:metabolism; D005341:Fibrinolysin / Q000494:pharmacology / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D010051:Ovarian Neoplasms / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology
D005337:Fibrin; D005340:Fibrinogen; D005176:Factor XIII; D010447:Peptide Hydrolases; D013917:Thrombin; D005341:Fibrinolysin