Validation of a simplified carbon-14-urea breath test for routine use for detecting Helicobacter [correction of Heliobacter] pylori noninvasively.
A carbon-14 (14C) urea breath test for detecting Helicobacter pylori with multiple breath sampling was developed. Carbon-14-urea (110 kBq) administered orally to 18 normal subjects and to 82 patients with Helicobacter infection. The exhaled 14C-labeled CO2 was trapped at 10-min intervals for 90 min. The total 14C activity exhaled over 90 min was integrated and expressed in %activity of the total dose given. In normals, a mean of 0.59% +/- 0.24% was measured, resulting in an upper limit of normal of 1.07%. In 82 patients, a sensitivity of 90.2%, a specificity of 83.8%, and a positive predictive value of 90.2% was found. The single probes at intervals of 40-60 min correlated best with the integrated result, with r ranging from 0.986 to 0.990. The test's diagnostic accuracy did not change at all when reevaluated with the 40-, 50-, or 60-min sample data alone. Thus, the 14C-urea breath test can be applied routinely as a noninvasive, low-cost and one-sample test with high diagnostic accuracy in detecting Helicobacter pylori colonization.
Henze
E
E
Division of Nuclear Medicine, University of Ulm, West Germany.
Malfertheiner
P
P
Clausen
M
M
Burkhardt
H
H
Adam
W E
WE
D000328:Adult; D000368:Aged; D001944:Breath Tests; D002245:Carbon Dioxide / Q000032:analysis*; D002250:Carbon Radioisotopes; D005260:Female; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000145:classification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D014508:Urea
D002250:Carbon Radioisotopes; D002245:Carbon Dioxide; D014508:Urea