Recoveries of Salmonella from soil in Eastern region of Saudi Arabia Kingdom.
The incidence and persistence of Salmonella in soils of different localities in Eastern region of Kingdom of Saudi Arabia (KSA) were investigated by using brilliant green agar (BGA) and Xylose lysine desoxycholate agar (XLDA) media, at 37 degrees C, 43 degrees C and 55 degrees C. No enrichment technique was used. A total of 220 soil samples were collected from agricultural and recreational areas. The positive recoveries of Salmonella were 6.36% at 37 degrees C and 10.5% at 43 degrees C on BGA medium. On XLDA medium the percentage of recoveries of Salmonella were 10.0% and 15.5 at 37 degrees C and 43 degrees C, respectively. No Salmonella was detected on both medium at 55 degrees C temperature. Jointly XLDA medium and 43 degrees C incubation temperature were favourable for recovery of Salmonella from soil samples. Recovery was higher from agricultural soil than recreational soils. The isolated individuals were tentatively classified into two group i) H2 S-producing and ii) H2S-non-producing. Salmonella individuals were sensitive to 50 micrograms/ml chloramphenicol, ampicillin, streptomycin nor tetracycline. But the same concentration of novobiocine and erythromycin reduced Salmonella H2S-non-producing; 30% and 20% for H2S-producing Salmonella. The treatment produced changes of color and size of colonies. This study confirm that the recycling of Salmonella sp. in nature; through human activities, polluted agricultural products and even recreational localities.
Abdel-Monem
M H
MH
Botany Department, Faculty of Science, Suez-Canal University, Ismailia, Egypt.
Dowidar
A
A
D003470:Culture Media; D008826:Microbial Sensitivity Tests; D008828:Microbiological Techniques; D012475:Salmonella / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D012529:Saudi Arabia; D012988:Soil Microbiology
D003470:Culture Media