Return of immunohematopoietic impairment a long time after murine syngeneic bone marrow transplantation.
We have tested the immunologic status and hematologic parameters of mice 2 months (short-term survivors) or 18 months (long-term survivors) after lethal total body irradiation and syngeneic bone marrow transplantation (SBMT), and of normal mice of corresponding age. Long-term SBMT survivors showed significantly lowered bone marrow and spleen cellularities, decreased numbers of CFU-S in hemopoietic organs and severe impairment in the formation of CFU-F colonies compared with short-term SBMT survivors and normal mice. The peripheral blood parameters (hematocrit, erythrocytes, reticulocytes, platelets, white blood cells and granulocyte counts), however, remained unaltered. In long-term SBMT survivors we also observed a relative increase of Lyt-2+ lymphocytes (CD8+, cytotoxic/suppressor) and Mac-1+ cells among splenocytes. At the same time the L3T4+/Lyt-2+ ratio (CD4+/CD8+) was decreased. Relative contents of Ig+, Thy-1+ and L3T4+ cells were unchanged. The ability of splenocytes to generate IL-2R+ cells after in vitro stimulation with concanavalin A was greatly diminished. In summary, the immunohematopoietic status after initial normalization is again impaired in long-term SBMT survivors.
Skórski
T
T
Department of Cytophysiology, Medical Center of Postgraduate Education, Warsaw, Poland.
Kawalec
M
M
Ratajczak
M
M
Szczylik
C
C
Kawiak
J
J
D000818:Animals; D001773:Blood Cells / Q000166:cytology / Q000276:immunology / Q000528:radiation effects; D001853:Bone Marrow / Q000528:radiation effects / Q000601:surgery; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000276:immunology* / Q000401:mortality / Q000473:pathology; D003208:Concanavalin A / Q000494:pharmacology; D006400:Hematocrit; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology* / Q000502:physiology; D016177:Macrophage-1 Antigen / Q000276:immunology; D008297:Male; D051379:Mice; D015375:Receptors, Interleukin-2 / Q000187:drug effects; D013154:Spleen / Q000166:cytology / Q000528:radiation effects / Q000648:ultrastructure; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000276:immunology; D013997:Time Factors; D014185:Transplantation, Isogeneic; D014916:Whole-Body Irradiation
D016177:Macrophage-1 Antigen; D015375:Receptors, Interleukin-2; D003208:Concanavalin A