Yields of fibronectin by heparin-cold-precipitation from plasma.
Fibronectin prepared by heparin-cold-precipitation from 10 packs each of 24-hour plasma, stored plasma and 6-hour frozen plasma was assayed by electroimmunodiffusion. Its yield obtained from 24-hour plasma was significantly higher than that from stored plasma (p less than 0.01) indicating a decline with storage. Freezing of plasma within 6 hours retards degradation of fibronectin. Therefore, 24-hour plasma or 6-hour frozen plasma should be used to prepare fibronectin in order to achieve a better yield.
Tien
S L
SL
National Blood Centre, Singapore Blood Transfusion Service.
Ong
Y W
YW
Lim
S H
SH
Chiang
T
T
D001793:Blood Preservation; D011232:Chemical Precipitation; D003080:Cold Temperature; D015925:Cryopreservation; D005353:Fibronectins / Q000302:isolation & purification*; D006493:Heparin; D006801:Humans; D005779:Immunodiffusion; D010949:Plasma / Q000737:chemistry*; D013048:Specimen Handling / Q000379:methods*; D013997:Time Factors
D005353:Fibronectins; D006493:Heparin