title: The effects of low density lipoproteins on uptake of Photofrin II.
abstract: The cellular uptake of Photofrin II (PII) was studied using fluorescence imaging and chemical extraction. The influence of serum and low density lipoproteins (LDL) was examined under a variety of experimental conditions employing cultured human cells of different origins as well as a subcutaneously SMT-F tumor implanted in mice. Results showed that serum inhibited PII uptake. In general, LDL also inhibits PII uptake with the exception of an initial increase in the first 10-30 min when the cellular concentration of PII was measured by fluorescence imaging instead of chemical extraction. Our results suggest a possible de-aggregation process occurring upon internalization or binding of PII to LDL.
authors: Cancer Imaging, British Columbia Cancer Research Centre, Vancouver, Canada.
authors: M
authors: M
authors: Korbelik
authors: J
authors: J
authors: Hung
authors: S
authors: S
authors: Lam
authors: B
authors: B
authors: Palcic
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002460:Cell Line; D017323:Dihematoporphyrin Ether; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism; D006415:Hematoporphyrins / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects / Q000378:metabolism
chemical_list: D006415:Hematoporphyrins; D008077:Lipoproteins, LDL; D017323:Dihematoporphyrin Ether