Electrotransfection of Escherichia coli with lambda gt10 DNA.
We have used electroporation to introduce lambda gt10 DNA into E. coli C600 (Electrotransfection). We obtained approximately 10(7) pfu (plaque forming units) per micrograms of lambda gt10 DNA. This frequency is 100-fold higher than the maximum reported for classical calcium chloride-induced transfection. We have also compared electrotransfection with in vitro packaging in cloning experiments using relatively small amounts (less than or equal to 10 ng) of DNA. Our results slow that electrotransfection can generate approximately 1000-fold more plaques than in vitro packaging at these concentrations.
Powell
R
R
Department of Microbiology, University College, Galway, Ireland.
Heery
D M
DM
Gannon
F
F
Dunican
L K
LK
D010582:Bacteriophage lambda / Q000235:genetics*; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D004560:Electricity; D004926:Escherichia coli / Q000235:genetics*; D014162:Transfection
D004279:DNA, Viral