Effect of gamma-interferon on phagosome-lysosome fusion in Salmonella typhimurium-infected murine macrophages.
Effect of recombinant gamma-interferon (rIFN-gamma) on phagosome-lysosome fusion in Salmonella typhimurium-infected murine macrophages was examined. rIFN-gamma enhanced phagosome-lysosome fusion in macrophages infected with S. typhimurium in a dose-dependent manner, and over a range of 10(2) to 10(3) U/ml of rIFN-gamma exhibited maximum phagosome-lysosome fusion, although phagocytosis was slightly decreased. The enhancement of phagosome-lysosome fusion occurred greater than 3 h post-treatment with rIFN-gamma. Furthermore, the macrophage activation for phagosome-lysosome fusion was found to persist for 4 days even when rIFN-gamma had been removed. These results demonstrate that IFN-gamma may serve as a mediator for the activation of phagosome-lysosome fusion in murine macrophages.
Ishibashi
Y
Y
Department of Microbiology, Meiji College of Pharmacy, Nozawa, Tokyo, Japan.
Arai
T
T
D000818:Animals; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000494:pharmacology*; D008247:Lysosomes / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects / Q000382:microbiology*; D008297:Male; D008561:Membrane Fusion / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D010588:Phagosomes / Q000187:drug effects*; D011994:Recombinant Proteins; D012486:Salmonella typhimurium / Q000276:immunology*
D011994:Recombinant Proteins; D007371:Interferon-gamma