Metabolism in vivo of 3,4,5,3',4'-pentachlorobiphenyl and toxicological assessment of the metabolite in rats.
Metabolism in vivo of 3,4,5,3',4'-pentachlorobiphenyl (PenCB) and toxicological assessment of the metabolite were investigated using male Wistar rats. Only one metabolite was isolated from the feces of rats administered 3,4,5,3',4'-PenCB. By gas chromatography-mass spectrometry, the methylated metabolite was identified with the synthesized authentic sample, 4'-methoxy-3,4,5,3',5'-PenCB. This indicated that the metabolite was 4'-hydroxy-3,4,5,3',5'-PenCB, which was produced via a 4',5'-epoxide formation and subsequent NIH-shift of the 4'-chlorine to the 5'-position. Administration of the metabolite at either single i.p. dose of 3 or 10 mg/kg to rats did not cause any toxic and biological effects such as body weight loss, atrophy of thymus and spleen, liver hypertrophy, increase of liver lipids, or 3-methylcholanthrene-type induction of liver enzymes. These changes were observed in rats administered with 3,4,5,3',4'-PenCB at a single dose of 3 mg/kg. In addition, a trace amount of 4'-hydroxy-3,4,5,3',5'-PenCB could be detected in rat liver 5 d after treatment with 3,4,5,3',4'-PenCB or 4'-hydroxy-3,4,5,3',5'-PenCB. The amount of this metabolite excreted in feces during 5 d after treatment with 3,4,5,3',4'-PenCB accounted for only 1.3% of dose. In 4'-hydroxy-3,4,5,3',5'-PenCB-treated rats, about 60% of dose was excreted as unchanged in feces for 5 d. These results suggest that this metabolite is a detoxified product and has no longer the high affinity for the liver, being excreted rapidly into the feces.
Koga
N
N
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Beppu
M
M
Yoshimura
H
H
D000818:Animals; D002855:Chromatography, Thin Layer; D004790:Enzyme Induction / Q000187:drug effects; D005243:Feces / Q000737:chemistry; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009929:Organ Size / Q000187:drug effects; D011078:Polychlorinated Biphenyls / Q000378:metabolism* / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains
C067423:4'-hydroxy-3,4,5,3',5'-pentachlorobiphenyl; D011078:Polychlorinated Biphenyls; C023035:3,4,5,3',4'-pentachlorobiphenyl