title: The GC-selective ligand mithramycin alters the structure of (AT)n sequences flanking its binding sites.
abstract: DNA fragments containing (AT)n inserts cloned adjacent to putative mithramycin binding sites have been examined by footprinting experiments using a variety of nucleases in the presence of the drug. The results demonstrate that mithramycin induces a DNA structural change which renders adjacent (AT)n sequences sensitive to attack by DNase II. Significant changes are also revealed with DNase I and micrococcal nuclease. The results are consistent with a model in which mithramycin opens the DNA minor groove changing it to a structure which is locally more like A-DNA.
authors: Dept. Physiology & Pharmacology, University of Southampton, UK.
authors: B M
authors: BM
authors: Cons
authors: K R
authors: KR
authors: Fox
mesh_terms: D000225:Adenine; D001482:Base Composition; D001483:Base Sequence; D003001:Cloning, Molecular; D003596:Cytosine; D004247:DNA; D003851:Deoxyribonucleases; D006147:Guanine; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D010957:Plasmids; D008926:Plicamycin; D013941:Thymine
chemical_list: D006147:Guanine; D003596:Cytosine; D004247:DNA; D003851:Deoxyribonucleases; D000225:Adenine; D008926:Plicamycin; D013941:Thymine