title: [The assessment of the toxigenicity of clinical strains of Pseudomonas aeruginosa by an immunoenzyme method using nitrocellulose filters].
abstract: The enzyme immunoassay (EIA) on nitrocellulose filters was adapted for the detection of exotoxin A in 104 P. aeruginosa strains isolated from patients and the environment in surgical wards in hospitals of Moscow and Alma-Ata. The method was shown to be highly sensitive: it permitted the detection of 5.ng of P. aeruginosa exotoxin A. The screening of 104 P. aeruginosa clinical strains by means of EIA on nitrocellulose filters revealed that these strains exotoxin A in 88.5% of cases.
authors: N S
authors: NS
authors: Brodinova
authors: A D
authors: AD
authors: Aleksandrov
authors: G G
authors: GG
authors: Kitarova
authors: A F
authors: AF
authors: Moroz
mesh_terms: D003101:Collodion; D004783:Environmental Microbiology; D005098:Exotoxins / Q000032:analysis*; D005374:Filtration / Q000295:instrumentation; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D007623:Kazakhstan; D009031:Moscow; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification / Q000472:pathogenicity*; D013503:Surgery Department, Hospital
chemical_list: D005098:Exotoxins; D003101:Collodion
vernacular_title: Otsenka toksigennosti klinicheskikh shtammov Pseudomonas aeruginosa immunofermentnym metodom s ispol'zovaniem nitrotselliuloznykh fil'trov.