Aberrant splicing of a naturally occurring alcohol dehydrogenase null activity allele in Drosophila melanogaster.
The DNA sequence of a naturally occurring alcohol dehydrogenase null activity allele, AdhnAC14, has eight extra nucleotides (in two groups of four) in the second intron, commencing six bases 3' from the 5' splice site. A stop codon was also found in exon 2. S1 nuclease protection experiments have shown that the insertions in intron 2 disrupt the correct splicing of intron 2. The null allele produces a transcript approximately 100 bases longer than the normal mature adult transcript, and the amount of the null allele transcript is only about 10% of the normal level.
Freeth
A L
AL
Molecular and Population Genetics Group, Research School of Biological Sciences, Autralian National University, Canberra.
Gibson
J B
JB
Wilks
A V
AV
D000426:Alcohol Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D000483:Alleles; D000818:Animals; D001483:Base Sequence; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D012326:RNA Splicing; D015183:Restriction Mapping
D000426:Alcohol Dehydrogenase