Identification and purification of a Bombyx mori homologue of FTZ-F1.
Extracts from embryos and from posterior and middle silk glands of the silkworm, Bombyx mori contain a sequence specific DNA binding factor termed BmFTZ-F1. The factor binds to the recognition site of FTZ-F1, a positive regulator of the fushi tarazu gene in Drosophila melanogaster. BmFTZ-F1 and FTZ-F1 share the same methylation interference patterns, the same chromatographic behaviors and similar protease digestion profiles. Anti-FTZ-F1 cross reacts with BmFTZ-F1. These results indicate that BmFTZ-F1 is a B. mori homologue of FTZ-F1. The mobility of the factor-DNA complex formed in the silk gland extract changes depending on the developmental stages. Purification of BmFTZF1 to an almost homogeneous state reveals that the factor is a 73 kd protein.
Ueda
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Genetic Stock Center, National Institute of Genetics, Mishima, Japan.
Hirose
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D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D012831:Bombyx / Q000032:analysis* / Q000033:anatomy & histology / Q000196:embryology / Q000235:genetics; D003429:Cross Reactions; D004268:DNA-Binding Proteins / Q000235:genetics; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics; D051717:Fushi Tarazu Transcription Factors; D018398:Homeodomain Proteins; D007301:Insect Hormones / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D008745:Methylation; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012689:Sequence Homology, Nucleic Acid; D012697:Serine Endopeptidases / Q000378:metabolism
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D051717:Fushi Tarazu Transcription Factors; D018398:Homeodomain Proteins; D007301:Insect Hormones; C497169:ftz protein, Drosophila; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase