Interleukin-2 and neopterin-induced neopterin release from peripheral blood mononuclear cells.
Stimulation of peripheral blood mononuclear cells (PBMC) by the mitogenic lectins, phytohaemagglutinin (PHA) and concanavalin A (conA), the lymphokine gamma-interferon (gamma-IFN) and interleukin-2 (IL-2) and the pterin neopterin, caused an increased release of neopterin from those cells, with peak levels after 7 days of stimulation. In contrast to gamma-IFN, IL-2 and neopterin failed to induce neopterin release from purified macrophages. IL-2- and neopterin-induced release of neopterin from PBMC is not dependent on proliferation and is partially inhibited by the addition of anti gamma-IFN or anti IL-2 receptor. Neopterin autoinductive production can explain the amplificated neopterin release during activation of the cellular-mediated immune response (CMI), in spite of the decrease in the T helper cell subsets, which are the main gamma-IFN producers.
Barak
M
M
Biochemistry Department, Carmel Hospital, Haifa, Israel.
Merzbach
D
D
Gruener
N
N
D001708:Biopterins / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism / Q000494:pharmacology; D002455:Cell Division; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology*; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008934:Mitogens / Q000494:pharmacology; D019798:Neopterin
D007376:Interleukin-2; D008934:Mitogens; D001708:Biopterins; D019798:Neopterin; D007371:Interferon-gamma