Formation of UDP-2-acetamido-2-deoxy-L-galactose and UDP-2-acetamido-2-deoxy-L-galacturonic acid by Pseudomonas aeruginosa.
The O-specific polysaccharide from the lipopolysaccharide of Pseudomonas aeruginosa NCTC 8505 (IATS serotype O:3) consists of a tetrasaccharide repeating unit comprising L-rhamnose, N-acetyl-D-glucosamine (GlcNAc), bacillosamine, and N-acetyl-L-galactosaminuronic acid (L-GalNAcA) (Y. Tahara and S. G. Wilkinson, Eur. J. Biochem. 134:299-304, 1983). Incubation of GlcN or UDP-GlcNAc with cell extracts or EDTA-treated cells of P. aeruginosa NCTC 8505 yielded a mixture of UDP-ManNAc, UDP-GalNAc, UDP-GlcNAcA, UDP-ManNAcA, UDP-L-GalNAc, and UDP-L-GalNAcA. The last two compounds, here identified for the first time, may be intermediates in the synthesis of the L-GalNAcA moiety of the O-specific portion of the lipopolysaccharide of P. aeruginosa.
Singh
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Department of Microbiology, Biochemistry, and Molecular Biology, School of Medicine, University of Pittsburgh, Pennsylvania 15261.
Singh
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Hogan
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Feingold
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D005944:Glucosamine / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D014536:Uridine Diphosphate N-Acetylgalactosamine / Q000378:metabolism*; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism*; D014539:Uridine Diphosphate Sugars / Q000378:metabolism*
D008070:Lipopolysaccharides; D014539:Uridine Diphosphate Sugars; C062239:UDP-2-acetamido-2-deoxygalacturonic acid; D014537:Uridine Diphosphate N-Acetylglucosamine; D014536:Uridine Diphosphate N-Acetylgalactosamine; D010727:Phosphoric Diester Hydrolases; D005944:Glucosamine