Phosphorylation of the ETS-2 protein: regulation by the T-cell antigen receptor-CD3 complex.
Phosphorylation of the human ets-2 protein in response to mitogenic signals to T lymphocytes was investigated in Jurkat cells. Activation of the cells by antibodies against the T-cell antigen receptor-CD3 complex or by concanavalin A was followed within 5 min by increased phosphorylation of the protein, as shown by a mobility shift of the protein from 54 to 56 kilodaltons in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and increased incorporation of 32P. The Ca2+ ionophores A23187 and ionomycin were able to mimic this effect, suggesting that this phosphorylation is mediated by Ca2+.
Fujiwara
S
S
Laboratory of Molecular Oncology, National Cancer Institute, Frederick, Maryland 21701-1013.
Koizumi
S
S
Fisher
R J
RJ
Bhat
N K
NK
Papas
T S
TS
D000945:Antigens, Differentiation, T-Lymphocyte / Q000502:physiology*; D017252:CD3 Complex; D002118:Calcium / Q000502:physiology; D004268:DNA-Binding Proteins; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000378:metabolism*; D010766:Phosphorylation; D010768:Phosphoserine / Q000378:metabolism; D011493:Protein Kinase C / Q000502:physiology; D050787:Proto-Oncogene Protein c-ets-2; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D011948:Receptors, Antigen, T-Cell / Q000502:physiology*; D012097:Repressor Proteins; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000378:metabolism*; D015534:Trans-Activators; D014157:Transcription Factors; D014407:Tumor Cells, Cultured
D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D004268:DNA-Binding Proteins; C107465:ERF protein, human; C494069:ETS2 protein, human; D009687:Nuclear Proteins; D050787:Proto-Oncogene Protein c-ets-2; D011518:Proto-Oncogene Proteins; D011948:Receptors, Antigen, T-Cell; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D010768:Phosphoserine; D011493:Protein Kinase C; D002118:Calcium