Cloning of a chromosomal fragment from Lactococcus lactis subsp. lactis partially complementing Escherichia coli recA functions.
A recA-like gene was isolated from a gene library of Lactococcus lactis subsp. lactis by intergeneric complementation of an E. coli recA mutant. A plasmid was obtained which fully complemented the RecA response to DNA damaging agents and UV inducibility of prophage, but not P1 plating efficiency in an E. coli recA mutant. The cloned DNA fragment also partially complemented the rec mutation in Lc. lactis MMS36. Hybridization studies showed that there was no detectable sequence homology between the recA gene of E. coli and Lc. lactis subsp. lactis chromosomal DNA.
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Laboratoire de Génétique Microbienne, IRBA Université de Caen, France.
Huang
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D010582:Bacteriophage lambda / Q000235:genetics; D002876:Chromosomes, Bacterial / Q000648:ultrastructure*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry; D004351:Drug Resistance; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D015698:Genomic Library; D013294:Lactococcus lactis / Q000235:genetics*; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009154:Mutation; D011938:Rec A Recombinases / Q000096:biosynthesis / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D015183:Restriction Mapping; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D008937:Mitomycins; D011994:Recombinant Proteins; D016685:Mitomycin; D008741:Methyl Methanesulfonate; D011938:Rec A Recombinases