Examination of toxicity of diisopropylammonium dichloracetate (DADA), remedies for cardiac diseases, toward isolated rat hepatocytes.
Oxygen uptake (OU) and cell viability (CV) of isolated rat hepatocytes as a part of the indexes of hepatopathy were determined following treatment with DADA, the active principle of pangamic acid. DADA increased OU at high concentrations, but not at low ones. However, CV became higher as the concentration became lower. DADA led to no remarkable toxicity when used alone. When the mixture of DADA and calcium gluconate was tested, DADA showed no palliative action; nor did it when combined with other hepatotoxins such as trapidil, rifampicin, hexobarbital, thiopental sodium, and Citanest-octapressin.
Hatano
M
M
Department of Internal Medicine, Meikai University School of Dentistry, Saitama, Japan.
Katsu
K
K
Ishihara
M
M
D000818:Animals; D002125:Calcium Gluconate / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004338:Drug Combinations; D005259:Felypressin / Q000633:toxicity; D006591:Hexobarbital / Q000633:toxicity; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D008297:Male; D034442:N-substituted Glycines; D010101:Oxygen Consumption / Q000187:drug effects; D011318:Prilocaine / Q000633:toxicity; D011437:Propylamines; D000644:Quaternary Ammonium Compounds / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D012293:Rifampin / Q000633:toxicity; D013874:Thiopental / Q000633:toxicity; D014192:Trapidil / Q000633:toxicity; D014803:Vitamin B Complex / Q000633:toxicity*
D004338:Drug Combinations; D034442:N-substituted Glycines; D011437:Propylamines; D000644:Quaternary Ammonium Compounds; D011318:Prilocaine; D014803:Vitamin B Complex; D005259:Felypressin; C001002:diisopropylamine dichloroacetate; D006591:Hexobarbital; D014192:Trapidil; D013874:Thiopental; C105565:pangamic acid; D002125:Calcium Gluconate; D012293:Rifampin