Ovine haemopoiesis: the development of bone marrow-derived colony-forming cells in vitro in the presence of factors derived from lymphoid cells and helper T-cells.
Techniques for the development of ovine bone marrow-derived haemopoietic progenitor cells and in situ identification of colony morphology are described. Both mitogen stimulated lymphoid cells and antigen stimulated helper T-cells generated potent colony-stimulating activity in conditioned medium. Monocyte/macrophage, neutrophil, eosinophil, basophil/mast cell, neutrophil/monocyte and mixed phenotype colonies developed in stimulated bone marrow cultures in a conditioned medium dose-dependent manner. Neutrophil, monocyte/macrophage and eosinophil colonies were detected in greater numbers than the other types, with mixed colonies representing only around 1% of the total. Eosinophil colonies were particularly abundant when compared to published reports of the numbers obtained with similar cultures of 'normal' mouse or human bone marrow cells. This culture technique will allow a detailed analysis of both ovine colony-stimulating factors and of the distribution of haemopoietic progenitor cells in vivo.
Haig
D M
DM
Moredun Research Institute, Edinburgh, Great Britain.
Brown
D
D
MacKellar
A
A
D000818:Animals; D001854:Bone Marrow Cells; D002452:Cell Count; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000302:isolation & purification / Q000494:pharmacology*; D003470:Culture Media / Q000494:pharmacology; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects; D066298:In Vitro Techniques; D008221:Lymphoid Tissue / Q000032:analysis*; D010641:Phenotype; D012756:Sheep; D006377:T-Lymphocytes, Helper-Inducer / Q000032:analysis*
D003115:Colony-Stimulating Factors; D003470:Culture Media