Peripheral nerve CR1 limit complement-mediated haemolysis.
CR1 were purified from sciatic nerve extracts by monoclonal antibody affinity chromatography. The effect of CR1 on complement-mediated haemolysis was studied by adding purified CR1 to a mixture of human serum as source of complement and sheep erythrocytes sensitized with rabbit IgG. A dose-dependent inhibition of the haemolysis occurred. There was no effect on the haemolysis when phosphate-buffered saline, elution buffer or run-through fraction not containing CR1 was added. Addition of a polyclonal anti-CR1 antibody to purified CR1 suppressed the inhibiting activity, strongly indicating that peripheral nerve CR1 inhibit complement-mediated haemolysis. This may be of great importance in vivo since CR1 present on the Schwann cell membrane may prevent the formation of terminal lytic complexes.
Vedeler
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Broegelmann Research Laboratory for Microbiology, Bergen, Norway.
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D003165:Complement System Proteins / Q000276:immunology*; D006461:Hemolysis; D006801:Humans; D010525:Peripheral Nerves / Q000276:immunology*; D011951:Receptors, Complement / Q000502:physiology*; D017463:Receptors, Complement 3b
D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D003165:Complement System Proteins