Monoclonal anti-conjugated azelaic acid antibody production: application to multiple sclerosis.
We have previously reported the existence of anti-conjugated azelaic acid (Aze A) antibodies in the serum of patients with multiple sclerosis (MS). In order to demonstrate the specificity of these antibodies, we have produced a monoclonal antibody directed against Aze A conjugated by an acylation reaction to a protein. In competition experiments, with ELISA method, we demonstrated that a part of the antibodies, raised in rabbit after immunization by human immunoglobulins (Ig) of MS patients, recognized the antigen-combining site of our monoclonal anti-conjugated Aze A antibody. These results clearly demonstrate that a part of human Ig obtained from sera of MS patients shared common idiotopes with mouse monoclonal antibody raised against conjugated Aze A.
Chagnaud
J L
JL
Laboratoire d'Immunologie et Pathologie, CJF 88-13 INSERM, Université de Bordeaux II, France.
Gosset
I
I
Brochet
B
B
Audhuy
S
S
Geffard
M
M
D000215:Acylation; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000917:Antibody Formation; D000918:Antibody Specificity; D002459:Cell Fusion; D003998:Dicarboxylic Acids / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D051379:Mice; D008809:Mice, Inbred C3H; D009103:Multiple Sclerosis / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D012710:Serum Albumin, Bovine
D000911:Antibodies, Monoclonal; D003998:Dicarboxylic Acids; D012710:Serum Albumin, Bovine; C010038:azelaic acid