Alternative splice forms of the murine T-cell receptor beta transcript in a cytotoxic T-cell line.
The processing of the primary transcript of the murine T cell receptor c beta region can result in two distinct forms of protein. The optional exon, C beta O, is appended at the 5' end of the constant region and to date has been observed at low frequencies in cDNA clones from heterogeneous populations of T lymphocyte. Because individual cell lines were not analyzed in those studies, it was not known whether small numbers of T lymphocytes use the C beta O exon exclusively or instead most T-lymphocytes use the C beta O exon but at low levels. We have determined that our CTL clone, B6.cl 4, produces both C beta O+ and C beta O- functional RNA transcripts. To determine the C beta O usage in this CTL clone, we coupled cDNA synthesis with the polymerase chain reaction using oligonucleotides corresponding to sequences at the V beta 14 region and sequences corresponding to C beta O or C beta 1. The data indicate that B6.cl 4 CTL clone is able to use either splice site but uses the C beta O exon at a frequency of approximately 2-3%.
Schaar
D G
DG
Department of Pathology, University of Medicine & Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854.
Oh
B
B
Kallay
L M
LM
Doherty
P J
PJ
Pan
S H
SH
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D005091:Exons; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D016693:Receptors, Antigen, T-Cell, alpha-beta; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014158:Transcription, Genetic
D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta