Immuno-phenotype and proliferative response of B-cell chronic lymphocytic leukemia.
This study examines the immunophenotypic profiles in both pretreated and treated CLL patients which could be useful from the prognosis point of view. Patients suffering from B CLL and having IgG markers were relatively more aggressive than cells bearing IgM phenotypes. Male predominance is observed in male/female ratio in this disease. B CLL showed heterogeneity by showing reactivity against various T cell markers such as CD5 (present on mature T cells) and also CD4 (T helper/inducer), CD8 (T suppressor/cytotoxic) and a very high percentage of Ia (HLA-DR). The proliferative response to cells to stimulation with PHA and PWM indicated that there is a primary defect in the capacity of these small lymphocytes to undergo a proliferative response due to an intrinsic defect in the B lymphocytes. This study also reflects a maturation arrest in the later developmental stage of B lymphopoiesis. The three findings which are novel are the difference in prognosis between IgG-IgM and IgG, the changes in T cell subsets and the mitogenic response.
Karfa
S
S
Division of Laboratory Medicine, Tata Memorial Hospital, Parel, Bombay, India.
Gopal
R
R
Nadkarni
J S
JS
D000368:Aged; D016175:B-Lymphocyte Subsets / Q000276:immunology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D016130:Immunophenotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology* / Q000473:pathology; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016176:T-Lymphocyte Subsets / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer; D050378:T-Lymphocytes, Regulatory; D014407:Tumor Cells, Cultured
D007074:Immunoglobulin G; D007075:Immunoglobulin M