Affinity purification, biological characterization and serological evaluation of defined antigens from Mycobacterium tuberculosis.
Nine mycobacterial antigens have been purified by affinity chromatography using monoclonal antibodies (m. abs) as immunosorbent. The importance of the individual antigens have been evaluated by different immunological methods.
Ljungqvist
L
L
Mycobacteria Department, Statens Seruminstitut, Copenhagen, Denmark.
Andersen
A B
AB
Andersen
P
P
Hasløv
K
K
Worsaae
A
A
Bennedsen
J
J
Heron
I
I
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008815:Mice, Inbred Strains; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000276:immunology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D007106:Immune Sera