Analysis by DNA polymerase alpha activity of human breast tumour proliferation and the effect of endocrine therapy.
Cytosols of human breast tumours have been assayed for DNA dependent DNA polymerase alpha activity. DNA polymerase alpha activity in benign tumours was found to be significantly lower than in untreated malignant tumours. Biopsy samples removed surgically before and after endocrine therapy showed reduced DNA polymerase alpha activity in 6 out of 9 patients treated with 4-hydroxyandrostenedione, and in 6 out of 7 patients treated with MPA. DNA polymerase alpha activity in malignant breast tumours was higher in oestrogen receptor negative than oestrogen receptor positive tumours.
Coldham
N G
NG
Department of Chemical Pathology, St Mary's Hospital Medical School, London, UK.
Lai
L C
LC
Reed
M J
MJ
Ghilchik
M W
MW
Shaikh
N A
NA
James
V H
VH
D000735:Androstenedione / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001706:Biopsy; D001943:Breast Neoplasms / Q000188:drug therapy / Q000201:enzymology* / Q000648:ultrastructure; D004257:DNA Polymerase II / Q000378:metabolism*; D004967:Estrogens; D005260:Female; D006801:Humans; D008525:Medroxyprogesterone / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D017258:Medroxyprogesterone Acetate; D009376:Neoplasms, Hormone-Dependent / Q000188:drug therapy / Q000201:enzymology* / Q000648:ultrastructure; D011960:Receptors, Estrogen / Q000378:metabolism
D000970:Antineoplastic Agents; D004967:Estrogens; D011960:Receptors, Estrogen; D000735:Androstenedione; D017258:Medroxyprogesterone Acetate; D004257:DNA Polymerase II; D008525:Medroxyprogesterone; C014594:formestane