title: Interactive effects of dietary wheat bran and lard on N-methyl-N'-nitro-N-nitrosoguanidine-induced colon carcinogenesis in rats.
abstract: A 3 x 3 factorial experiment was conducted to examine how dietary fiber (wheat bran) and fat (lard) interactively affect the genesis of N-methyl-N'-nitro-N-nitrosoguanidine-induced colon cancer in rats. Groups of 30 male 4-week-old Wistar rats were fed ad libitum one of nine experimental diets containing either 15 (low), 27.5 (medium), or 40% (high) energy as fat in combination with 0.7 (low), 2.2 (medium), or 3.8 g (high) fiber/100 kcal for a period of 37 weeks. After 4 weeks, each rat received a total of five weekly intrarectal instillations of 6 mg N-methyl-N'-nitro-N-nitrosoguanidine/kg. The highest colon carcinoma incidence and the highest total number of carcinomas of the colon were observed in the animals fed the medium-fat/medium-fiber diet. The highest number of polyps and a relatively high polyp incidence occurred in the animals on the high-fat/low-fiber diet. An enhancing effect of fat on both the tumor incidence and tumor multiplicity was clearly present for the low-fiber diets, whereas fat had no effect when the fiber content of the diet was high. In general, the results showed a nonlinear dose-response relationship for fiber and fat. These results indicate that both dietary fiber and fat affect colon carcinogenesis in a complex, interactive manner.
authors: TNO-CIVO Toxicology and Nutrition Institute, Zeist, The Netherlands.
authors: E J
authors: EJ
authors: Sinkeldam
authors: C F
authors: CF
authors: Kuper
authors: M C
authors: MC
authors: Bosland
authors: V M
authors: VM
authors: Hollanders
authors: D M
authors: DM
authors: Vedder
mesh_terms: D000230:Adenocarcinoma / Q000139:chemically induced / Q000209:etiology*; D000818:Animals; D001835:Body Weight; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000209:etiology*; D003111:Colonic Polyps / Q000139:chemically induced / Q000209:etiology*; D004041:Dietary Fats / Q000008:administration & dosage*; D004043:Dietary Fiber / Q000008:administration & dosage*; D004435:Eating; D004734:Energy Metabolism; D008297:Male; D008769:Methylnitronitrosoguanidine; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004041:Dietary Fats; D004043:Dietary Fiber; D008769:Methylnitronitrosoguanidine