[Inducing effect of tumor necrosis factor-alpha on differentiation of U937 monocytic tumor cell line].
We used the human monocyte/macrophage-like tumor cell line U937 to examine whether tumor necrosis factor-alpha (TNF-alpha) induces differentiation of monocyte/macrophage progenitors. Incubation of these cells with recombinant human tumor necrosis factor-alpha (rhTNF-alpha) resulted in an increase in Fc rosette formation by the cells. RhTNF-alpha increased the rosette formation in dose- and culture time-dependent manners. And the highest Fc rosette formation was observed in culture medium supplemented with 10% serum. However, U937 cell growth was not inhibited by rhTNF-alpha. The stimulatory effect of rhTNF-alpha was completely inhibited when the factor and indomethacin (10(-6) M) were added simultaneously to the cells. Also, rhTNF-alpha increased NBT reducing activity of the cells. Additionally, rhTNF-alpha-treated U937 cells produced interleukin-1 in the cell density- and culture time-dependent fashions. These results demonstrate that rhTNF-alpha is a potent inducer of the differentiation of the monocyte/macrophage-like tumor cell line U937.
Hashimoto
H
H
Department of Oral Microbiology, Meikai University School of Dentistry, Saitama, Japan.
Hanazawa
S
S
D002454:Cell Differentiation / Q000187:drug effects*; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000096:biosynthesis; D008264:Macrophages; D009000:Monocytes; D012397:Rosette Formation; D014407:Tumor Cells, Cultured / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D014410:Tumor Stem Cell Assay
D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D007213:Indomethacin