title: Regulation of interleukin 2-driven T-lymphocyte proliferation by prolactin.
abstract: The requirement for prolactin in interleukin 2-driven T-cell proliferation was evaluated. Addition of an anti-prolactin antiserum resulted in the specific inhibition of T-cell proliferation in a time- and dose-dependent manner. Synthesis of prolactin and its mRNA, however, did not occur during interleukin 2 stimulation. Instead, previously internalized prolactin, presumably from fetal bovine serum, appears to serve as the source of prolactin under serum-free conditions. A 7-fold increase in a prolactin receptor occurred as a function of cell cycle progression; accumulation of a 1.6-kilobase prolactin receptor mRNA increased approximately 2-fold. Interleukin 2 stimulation induced the translocation of prolactin into the nucleus and prolactin receptor to the nuclear periphery. These data indicate that extracellular prolactin is requisite for T-cell proliferation and suggest that the effects of prolactin are exerted in the nucleus.
authors: Department of Pathology, University of Pennsylvania, Philadelphia 19104.
authors: C V
authors: CV
authors: Clevenger
authors: D H
authors: DH
authors: Russell
authors: P M
authors: PM
authors: Appasamy
authors: M B
authors: MB
authors: Prystowsky
mesh_terms: D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D003208:Concanavalin A; D005434:Flow Cytometry; D015870:Gene Expression; D007106:Immune Sera; D007376:Interleukin-2 / Q000494:pharmacology*; D008213:Lymphocyte Activation; D051379:Mice; D011388:Prolactin / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D011981:Receptors, Prolactin / Q000235:genetics / Q000502:physiology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
chemical_list: D007106:Immune Sera; D007376:Interleukin-2; D012333:RNA, Messenger; D011981:Receptors, Prolactin; D003208:Concanavalin A; D011388:Prolactin