title: Characterization of glucan involved in the reduction of dental caries in rats.
abstract: Culture supernatant fluids (SF) of mutant 108 obtained from Streptococcus sobrinus 6715, when incubated with sucrose, produce large amounts of water-soluble glucans. Mainly, two water-soluble glucan synthesizing glucosyltransferases (GTases) were found in culture SF by isoelectric focusing. These two GTases had higher activities than those from the parent strain. The GTase which synthesizes water-insoluble glucan totally lacked the mutant. When the glucan preparation synthesized by the SF was subjected to gel filtration, a major large molecular weight fraction (designated glucan A) and a small molecular weight fraction (designated glucan B) were obtained. Glucan A yielded a smaller molecular weight glucan after treatment with dextranase. Glucan B was not hydrolyzed by the same enzyme.
authors: Department of Microbiology, Nihon University School of Dentistry, Matsudo, Chiba, Japan.
authors: K
authors: K
authors: Takada
authors: T
authors: T
authors: Shiota
authors: T
authors: T
authors: Ikeda
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D003731:Dental Caries / Q000517:prevention & control*; D003910:Dextranase / Q000378:metabolism; D005936:Glucans / Q000096:biosynthesis* / Q000302:isolation & purification / Q000627:therapeutic use; D005964:Glucosyltransferases / Q000378:metabolism*; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D008745:Methylation; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009154:Mutation; D051381:Rats; D013291:Streptococcus / Q000201:enzymology* / Q000235:genetics
chemical_list: D005936:Glucans; D005964:Glucosyltransferases; D003910:Dextranase