The induction of human B-cell activation, proliferation and differentiation by anti-CD3-stimulated T cells--a model of T cell/B cell collaboration.
It is clear that anti-CD3-activated T cells provide all the signals necessary for polyclonal activation, proliferation and differentiation of human B cells. B-cell activation, proliferation and differentiation are initiated by a complex series of receptor-ligand interactions, of which LFA-1-ICAM-1 ligation plays a central, but not exclusive role. In addition, the action of a number of cytokines, most prominently IL2, is essential for B-cell proliferation and differentiation. It is apparent from the results obtained using this model system that the conjugate formation that occurs as part of antigen presentation and T-cell activation is not required for the subsequent collaboration between activated T cells and B cells necessary to trigger B-cell responses. The implication of these studies is that after initial antigen recognition and T-cell activation, T cell/B cell collaboration leading to antibody production is antigen non-specific and potentially capable of inducing polyclonal B-cell responses. Conjugate formation that develops as part of antigen presentation may tend to focus the response and limit the B cells that can be induced to respond. Alternatively, T-cell recognition of antigen may deliver a signal to the B cell by cross-linking MHC molecules bearing the recognized antigenic fragment that makes it more capable of responding to the T-cell-derived antigen-non-specific signals and cytokines promoting B-cell activation, proliferation and differentiation.
Lipsky
P E
PE
Harold C. Simmons Arthritis Research Center, University of Texas Southwestern Medical Center, Dallas 75235.
D000945:Antigens, Differentiation, T-Lymphocyte; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D017252:CD3 Complex; D002454:Cell Differentiation; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D008954:Models, Biological; D011948:Receptors, Antigen, T-Cell; D011971:Receptors, Immunologic; D013601:T-Lymphocytes / Q000276:immunology
D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D011948:Receptors, Antigen, T-Cell; D011971:Receptors, Immunologic