Three-dimensional structure of the ATPase fragment of a 70K heat-shock cognate protein.
The three-dimensional structure of the amino-terminal 44K ATPase fragment of the 70K bovine heat-shock cognate protein has been solved to a resolution of 2.2 A. The ATPase fragment has two structural lobes with a deep cleft between them; ATP binds at the base of the cleft. Surprisingly, the nucleotide-binding 'core' of the ATPase fragment has a tertiary structure similar to that of hexokinase, although the remainder of the structures of the two proteins are completely dissimilar, suggesting that both the phosphotransferase mechanism and the substrate-induced conformational change intrinsic to the hexokinases may be used by the 70K heat shock-related proteins.
Flaherty
K M
KM
Beckman Laboratories for Structural Biology, Department of Cell Biology, Stanford University School of Medicine, California 94305-5400.
DeLuca-Flaherty
C
C
McKay
D B
DB
D000251:Adenosine Triphosphatases; D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003198:Computer Simulation; D003460:Crystallization; D006360:Heat-Shock Proteins; D006593:Hexokinase; D046911:Macromolecular Substances; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D008970:Molecular Weight; D009994:Osmolar Concentration; D010446:Peptide Fragments; D011487:Protein Conformation; D014961:X-Ray Diffraction
D006360:Heat-Shock Proteins; D046911:Macromolecular Substances; D010446:Peptide Fragments; D000255:Adenosine Triphosphate; D006593:Hexokinase; D000251:Adenosine Triphosphatases