Streptozotocin-diabetes but not galactose-feeding increases the serum concentration of type IV collagen antigens in rats.
Serum concentrations of NC-1 (antigens related to the carboxy-terminal non-collagenous domain of type IV procollagen) were found to be increased significantly above normal in short-term (up to eight weeks duration) streptozotocin diabetic rats despite normal steady-state levels of the alpha 1 (IV) collagen mRNA previously reported in the kidneys of the same animals. The effect of long-term (11 and 28 weeks) diabetes on serum NC-1 was compared to the effects of galactose feeding (50% w/w) with and without aldose reductase inhibition (Statil). Serum concentrations in long-term diabetic animals remained significantly elevated above control levels at both time points. Normal NC-1 levels were found in galactosemic rats although this treatment is known to lead to capillary basement membrane thickening. Statil was proven to be effective in preventing polyol accumulation in sciatic nerve samples from diabetic rats but did not decrease the elevated serum NC-1 concentrations. These results suggest etiological heterogeneity in the development of different aspects of diabetic late complications and demonstrate no involvement of the polyol pathway in the events leading to increased serum NC-1 levels in diabetes.
Danne
T
T
Children's Hospital, Free University Berlin, F.R.G.
Poulsom
R
R
Schuppan
D
D
Prockop
D J
DJ
Boot-Handford
R P
RP
D000375:Aging; D000818:Animals; D000941:Antigens / Q000032:analysis*; D003094:Collagen / Q000097:blood*; D003921:Diabetes Mellitus, Experimental / Q000097:blood*; D004040:Dietary Carbohydrates / Q000494:pharmacology*; D005690:Galactose / Q000494:pharmacology*; D008297:Male; D011347:Procollagen / Q000032:analysis*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
D000941:Antigens; D004040:Dietary Carbohydrates; D011347:Procollagen; D003094:Collagen; D005690:Galactose