title: Stimulation of rat red blood cell glycolysis by phenylhydrazine hydrochloride.
abstract: The effects of phenylhydrazine hydrochloride (5, 10 and 20 mM) on the energy metabolism of red cells of rats were studied. There were found an 1.3-2-fold increase of lactate formation, an up to seven-fold accumulation of pyruvate and of the flux via the oxidative pentose pathway. The stimulation of the glycolysis was caused by 1) a large decline of the ATP concentration and a corresponding increase of ADP and AMP, and 2) by the stimulation of the actual activities of the Na+,K(+)-ATPase and Ca+(+)-ATPase owing to a greater permeability of the plasma membrane. A significant increase of the Ca+(+)-influx was demonstrated. A decline of the sum of adenine nucleotides after 60 and 90 min of incubation with phenylhydrazine was not accompanied by accumulation of hypoxanthine.
authors: Institute of Physiology, Faculty of Medicine, University Svetozar Markovic, Kragujevac, Yugoslavia.
authors: M M
authors: MM
authors: Kostić
authors: L
authors: L
authors: Dragićević
authors: R
authors: R
authors: Zirković
authors: M
authors: M
authors: Müller
authors: S M
authors: SM
authors: Rapoport
mesh_terms: D000227:Adenine Nucleotides / Q000097:blood / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000097:blood / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000097:blood / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006019:Glycolysis / Q000187:drug effects*; D007773:Lactates / Q000097:blood / Q000378:metabolism; D019344:Lactic Acid; D010427:Pentose Phosphate Pathway; D010659:Phenylhydrazines / Q000494:pharmacology*; D011773:Pyruvates / Q000097:blood / Q000378:metabolism; D019289:Pyruvic Acid; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000227:Adenine Nucleotides; D007773:Lactates; D010659:Phenylhydrazines; D011773:Pyruvates; C030299:phenylhydrazine; D019344:Lactic Acid; D019289:Pyruvic Acid; D000252:Calcium-Transporting ATPases; D002118:Calcium