Purification and characterization of a 26-kDa competitive inhibitor of interleukin 1.
The urine of patients with fever above 39 degrees C contains an inhibitor of interleukin 1. Herein we describe the purification of the interleukin 1 inhibitor by ion-exchange chromatography, hydrophobic chromatography, gel filtration and negative immunosorption. The purified protein has a molecular weight of 26,000; its size is reduced to 24,000 upon treatment with endoglycosidase F. The IL 1 inhibitor is a competitive inhibitor and acts by binding to the IL 1 receptor. The inhibitor recognizes the murine and human IL 1 receptor with similar affinity. The purified IL 1 inhibitor has a specific activity of 3 x 10(7) to 4.5 x 10(7) units/mg as tested on the human astrocytoma (U-373) cell proliferation bioassay and murine EL4.6.1 cell IL 1 receptor-binding assay.
Mazzei
G J
GJ
Glaxo Institute for Molecular Biology S.A., Geneva, Switzerland.
Seckinger
P L
PL
Dayer
J M
JM
Shaw
A R
AR
D001667:Binding, Competitive; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D005334:Fever / Q000652:urine; D006023:Glycoproteins / Q000378:metabolism / Q000652:urine*; D006031:Glycosylation; D006801:Humans; D007163:Immunosorbent Techniques; D007375:Interleukin-1 / Q000037:antagonists & inhibitors*; D008213:Lymphocyte Activation; D008970:Molecular Weight; D011971:Receptors, Immunologic / Q000378:metabolism*; D017472:Receptors, Interleukin-1
D006023:Glycoproteins; D007375:Interleukin-1; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1