Calcium-dependent phospholipid catabolism and arachidonic acid mobilization in cerebral minces.
Cerebral minces were used to investigate the role of calcium influx on trauma-induced alterations of brain lipid metabolism. Cerebral phospholipids, nonpolar lipids, and free fatty acids were radiolabeled in vivo with [3H]arachidonic acid. Tissue incubation stimulated the time-dependent catabolism of choline and inositol glycerophospholipids, and resulted in the accumulation of [3H]free fatty acids. These effects were attenuated in Ca2(+)-free incubations, and when EGTA or verapamil were present. The inhibition of calcium influx also reduced the labeling of diglycerides, whereas ethanolamine and serine glycerophospholipids were not affected by incubation or treatments. Replacing Ca2+ with other cations also attenuated the incubation-dependent alterations in lipid metabolism. However, only cadmium was able to compete with calcium and reduce the accumulation of [3H]free fatty acids. It appeared that about half of the observed phospholipid catabolism was dependent on Ca2+ influx and that at least 80% of the [3H]free fatty acid accumulation required calcium.
Damron
D S
DS
Dept. Biological Sciences, Kent State University, OH 44242.
Dorman
R V
RV
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D001930:Brain Injuries / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology* / Q000502:physiology; D004533:Egtazic Acid / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D010743:Phospholipids / Q000378:metabolism*; D011865:Radioisotope Dilution Technique; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium; D014700:Verapamil / Q000494:pharmacology
D001095:Arachidonic Acids; D010743:Phospholipids; D014316:Tritium; D016718:Arachidonic Acid; D004533:Egtazic Acid; D014700:Verapamil; D002118:Calcium