The ram: a novel low molecular weight GTP-binding protein cDNA from a rat megakaryocyte library.
A novel low Mr GTP-binding protein cDNA was isolated from a rat megakaryocyte cDNA library with a synthetic oligonucleotide probe corresponding to an 8-amino acid sequence specific for c25KG, a GTP-binding protein previously isolated from human platelet cytosol fraction [(1989) J. Biol. Chem. 264, 17000-17005]. The cDNA has an open reading frame encoding a protein of 221 amino acids with a calculated Mr of 25068. The protein is designated as ram (ras-related gene from megakaryocyte) protein (ram p25). The amino acid sequence deduced from the ram cDNA contains the consensus sequences for GTP-binding and GTPase domains. ram p25 shares about 23%, 39% and 80% amino acid homology with the H-ras, smg25A and c25KG proteins, respectively. The 3.5-kb ram mRNA was detected abundantly in spleen cells.
Nagata
K
K
Department of Biochemistry, Gifu University School of Medicine, Japan.
Satoh
T
T
Itoh
H
H
Kozasa
T
T
Okano
Y
Y
Doi
T
T
Kaziro
Y
Y
Nozawa
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D019204:GTP-Binding Proteins / Q000235:genetics*; D008533:Megakaryocytes / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D015183:Restriction Mapping; D020691:rab GTP-Binding Proteins; D000075745:rab27 GTP-Binding Proteins
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D000075745:rab27 GTP-Binding Proteins; D004247:DNA; D019204:GTP-Binding Proteins; C512962:Rab27a protein, mouse; C512961:Rab27a protein, rat; D020691:rab GTP-Binding Proteins