Cytokine control of peripheral-blood CD23 expression and sCD23 release: differential regulation by IL-2 and IL-4.
CD23 expression on peripheral-blood lymphocytes (PBL) was studied under the influence of cytokines. It is shown that IL-2 induced CD23 expression on human peripheral-blood B cells. Evidence is presented that the IL-2 induced CD23 expression and release of soluble CD23 (sCD23) are not mediated by IL-4. In comparison to IL-4, the IL-2-induced CD23 expression and sCD23 release revealed kinetic differences and were not inhibited by anti-IL-4. The prestimulation of PBL with Staphylococcus aureus strains Cowan 1 (SAC) led to a pronounced reduction in basal CD23 expression and to a change in the response of cytokines. Subsequent stimulation with IL-4 induced CD23 to the same extent as on unstimulated cells, whereas the IL-2-induced CD23 expression and sCD23 release were greatly reduced. Interferon gamma showed no effects on the IL-4-stimulated CD23 expression of SAC-PBL, whereas the IL-2-induced CD23 expression was suppressed. Furthermore, we demonstrate that the stimulation with IL-2/IL-4 inhibits the effects of the individual cytokine; this inhibition is also seen for immunoglobulin (E, G, M) synthesis.
Fischer
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A
Institut für Medizinische Mikrobiologie und Immunologie, Arbeitsgruppe für Infektabwehrmechanismen, Ruhr-Universität Bochum, BRD.
Pfeil
T
T
König
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W
D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis / Q000096:biosynthesis*; D002478:Cells, Cultured; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis; D007371:Interferon-gamma / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000494:pharmacology; D008214:Lymphocytes / Q000276:immunology; D011961:Receptors, Fc / Q000032:analysis / Q000096:biosynthesis*; D017455:Receptors, IgE
D000944:Antigens, Differentiation, B-Lymphocyte; D007136:Immunoglobulins; D007376:Interleukin-2; D015850:Interleukin-6; D011961:Receptors, Fc; D017455:Receptors, IgE; D015847:Interleukin-4; D007371:Interferon-gamma