Identification and characterization of phospholipase A2 inhibitory proteins in human mononuclear cells.
Calcium-dependent phospholipid binding and phospholipase A2 inhibitory proteins were isolated from human mononuclear cells. Lipocortins I and II were present whereas lipocortin IV (endonexin I) was not. The other proteins were purified to homogeneity and shown to have molecular masses of 35, 36, 32 and 73 kDa. The 36-kDa and 73-kDa proteins are related, the smaller appears to be part of the larger. The 73-kDa protein is related to the 67-kDa calelectrin and to lipocortin VI; the 32-kDa protein is different from endonexin I but related to chromobindin 7 and to lipocortin V. The 35-kDa protein has been identified by tryptic peptide sequencing as lipocortin III. All these proteins inhibit phospholipase A2 activity in vitro and the three smaller ones inhibit the [3H]arachidonic acid release from prelabelled monocytes induced by the calcium ionophore A23187 in a dose-dependent manner.
Coméra
C
C
Institut National de la Santé et de la Recherche Médicale, U129, Paris, France.
Rothhut
B
B
Russo-Marie
F
F
D000595:Amino Acid Sequence; D017314:Annexin A4; D017304:Annexin A5; D017302:Annexins; D000937:Antigen-Antibody Reactions; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D015153:Blotting, Western; D000001:Calcimycin / Q000494:pharmacology; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D007525:Isoelectric Focusing; D007963:Leukocytes, Mononuclear / Q000032:analysis* / Q000187:drug effects; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis; D010449:Peptide Mapping; D010740:Phospholipases / Q000037:antagonists & inhibitors*; D010741:Phospholipases A / Q000037:antagonists & inhibitors*; D054467:Phospholipases A2; D010743:Phospholipids / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014357:Trypsin
D017314:Annexin A4; D017304:Annexin A5; D017302:Annexins; D001095:Arachidonic Acids; D002135:Calcium-Binding Proteins; D010446:Peptide Fragments; D010743:Phospholipids; D016718:Arachidonic Acid; D000001:Calcimycin; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D014357:Trypsin