Mutation of a gene that encodes a kinesin-like protein blocks nuclear division in A. nidulans.
In A. nidulans, the temperature-sensitive cell cycle mutation bimC4 causes an elevated mitotic index at restrictive temperature. Under restrictive conditions the mutation interferes with separation of the spindle pole bodies, causes abnormal spindle morphology, and prevents nuclear division. We have cloned and sequenced the wild-type bimC gene. The predicted protein product has homology to Drosophila kinesin heavy chain. We conclude that this kinesin-like protein has an important role in nuclear division in Aspergillus.
Enos
A P
AP
Department of Pharmacology, UMDNJ-Robert Wood Johnson Medical School, Piscataway 08854.
Morris
N R
NR
D000251:Adenosine Triphosphatases / Q000235:genetics*; D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000166:cytology / Q000235:genetics*; D001483:Base Sequence; D002467:Cell Nucleus / Q000648:ultrastructure*; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D016547:Kinesins; D046911:Macromolecular Substances; D008938:Mitosis; D008940:Mitotic Index; D008969:Molecular Sequence Data; D009154:Mutation; D012689:Sequence Homology, Nucleic Acid
D005656:Fungal Proteins; D046911:Macromolecular Substances; D000251:Adenosine Triphosphatases; D016547:Kinesins