Use of column switching for the determination of niacinamide in compound feed.
A high-performance liquid chromatographic method is described for the determination of niacinamide in compound feed. The niacinamide is extracted with 0.2 M hydrochloric acid in order to suppress the hydrolysis of the niacinamide to nicotinic acid. The chromatography is carried out with the aid of column switching. An RP-18 column is used for the preseparation, and the analytical separation takes place in a cation-exchange column. With the proposed method, the limit of determination is about 2 ppm of niacinamide.
Balschukat
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D
Degussa AG, Industrial and Fine Chemicals Division, Hanau, F.R.G.
Kress
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E
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009536:Niacinamide / Q000032:analysis*; D011200:Poultry; D013552:Swine
D009536:Niacinamide