[Nucleotide sequence of the fragment of transcriptionally active locus in rat genome and analysis of a putative protein product].
Part of the transcriptionally active rat genomic locus, K51, has been sequenced (2,321 b.p.). The coding DNA chains was identified and two long open reading frames (ORF) were found by computer analysis one of which seems to be much less probable. By transcriptional mapping, at least two exons (A - about 110 b.p., B - 549 b.p.) were revealed. Computer search showed that the deciphered nucleotide and amino acid sequence was absent in the data-banks up to the end of 1988. Numerous GC rich direct repeats were noticed in the fragment. At protein level, resemblance with hypervariable N- and C-terminal domains of cytokeratins was visualized. Some regions of the putative protein product possess relatedness with some other gene products, particularly with ATP binding domains of mos oncoprotein.
Shvets
Iu P
IuP
Chumakov
I M
IM
Kiselev
L L
LL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015252:Deoxyribonucleases, Type II Site-Specific; D008969:Molecular Sequence Data; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D017231:Proto-Oncogene Proteins c-mos; D011519:Proto-Oncogenes; D051381:Rats; D015183:Restriction Mapping; D014158:Transcription, Genetic
D011518:Proto-Oncogene Proteins; D017231:Proto-Oncogene Proteins c-mos; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific
Nukleotidnaia posledovatel'nost' uchastka transkriptsionno aktivnogo lokusa K51 genoma krysy i analiz predpolagaemogo belkovogo produkta.