title: Migration assay for endothelial cells in multiwells. Application to studies on the effect of opioids.
abstract: An assay system is described which permits rapid and effective evaluation of endothelial cell repair, using cells growing in a monolayer. With this method it was possible to obtain highly significant results. For example, endothelial growth factor and heparin, significantly enhanced cell migration and/or proliferation, whereas beta-endorphin, an endogenous opioid, had no effect on the migration and/or proliferation of human umbilical vein endothelial cells. This model may be used to study the cell migration of a variety of cell types which under certain experimental conditions (e.g., irradiation) do not proliferate.
authors: Institute of Pathology, RWTH-Klinikum, Aachen, F.R.G.
authors: E G
authors: EG
authors: Fischer
authors: A
authors: A
authors: Stingl
authors: C J
authors: CJ
authors: Kirkpatrick
mesh_terms: D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D016228:Endothelial Growth Factors; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects; D006133:Growth Substances / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D008722:Methods; D008954:Models, Biological; D012038:Regeneration; D014945:Wound Healing; D001615:beta-Endorphin / Q000494:pharmacology*
chemical_list: D016228:Endothelial Growth Factors; D006133:Growth Substances; D001615:beta-Endorphin; D006493:Heparin