Raman study of the lenses of spontaneously-occurring and streptozotocin-induced diabetic rats.
The WBN/Kob rat is an animal model of spontaneously occurring diabetes only in males at the age of around one year. The diabetic cataract develops about a half year after the onset of diabetes. Using laser Raman spectroscopy we monitored WBN/Kob rat lenses which had chronic hyperglycemic stress and streptozotocin-induced diabetic rat lenses which showed relatively acute cataract formation. WBN/Kob rat lenses revealed a marked increase in the lens water content only in the cortical portion. On the other hand, streptozotocin-induced diabetic rat lenses showed a pronounced increase in the lens water content both in the cortical and the nuclear portion.
Toshima
S
S
Department of Ophthalmology, Jikei University School of Medicine, Tokyo, Japan.
Miyazaki
H
H
Mizuno
A
A
D000818:Animals; D001834:Body Water / Q000378:metabolism*; D002386:Cataract / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004195:Disease Models, Animal; D007908:Lens, Crystalline / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013059:Spectrum Analysis, Raman; D013311:Streptozocin
D013311:Streptozocin