Study of spindle microtubule reassembly in cells from Alzheimer and Down syndrome patients following exposure to colcemid.
Numerous intraneuronal neurofibrillary tangles and senile (neuritic) plaques are the two characteristic lesions in Alzheimer disease (AD) and adult Down syndrome (DS). Evidence indicates that microtubule assembly is impaired in AD. We studied spindle microtubule repolymerization rates in EBV-transformed lymphoblasts from AD, DS, and control individuals after colcemid exposure. The distinctive arrangement of microtubules in spindle and its size make this structure an obvious choice for study. Recovery trends in the three patient groups differed significantly; in particular, the controls showed an earlier appearance of intact spindle microtubules than AD. Other researchers found similar results using AD fibroblasts. The results from the DS cells were inconsistent and difficult to interpret. It is unclear how the AD microtubules differ from controls, or whether a relationship exists between the altered microtubule repolymerization kinetics observed in this study and the presence of neurofibrillary tangles in AD patients. A difference in repolymerization rates can be the basis for a diagnostic test for AD if it can be verified.
Krawczun
M S
MS
Department of Genetics, New York State Institute for Basic Research in Developmental Disabilities, Office of Mental Retardation and Developmental Disabilities, Staten Island 10314.
Jenkins
E C
EC
Lele
K P
KP
Sersen
E A
EA
Wisniewski
H M
HM
D000544:Alzheimer Disease / Q000175:diagnosis* / Q000235:genetics; D003584:Cytological Techniques; D003703:Demecolcine / Q000494:pharmacology*; D004314:Down Syndrome / Q000175:diagnosis* / Q000235:genetics; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D008297:Male; D008870:Microtubules / Q000187:drug effects* / Q000648:ultrastructure; D008941:Spindle Apparatus / Q000187:drug effects / Q000648:ultrastructure*
D003703:Demecolcine