Microsomal protein mediates a pH-dependent fusion of liposomes to rat brain microsomes.
The fusion between rat brain microsomes and liposomes is investigated by measuring the release of octadecylrhodamine B (R18) fluorescence self-quenching. In the experimental conditions used in this work, the method allows a rapid and quantitative evaluation of the mixing of microsome and liposome lipid phases. The decrease of pH below 7 produces an extensive fusion between microsomes and acidic phospholipid liposomes. Microsomal protein is necessary for fusion, which is inactivated by exposure of microsomes to pronase. Therefore, H(+)-induced fusion differs from Ca(2+)-induced fusion since the latter does not require microsomal protein. The pretreatment of microsomes with trinitrobenzenesulfonic acid (TNBS) in nonpenetrating conditions does not affect the extent of fusion. On the other hand, N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ), a reagent able to react with carboxyl groups, causes an extensive inactivation of fusion. Therefore, the H(+)-induced fusion described here depends on some microsomal protein and may have physiological significance because it occurs at pH values present in the living cell. H(+)-dependent fusion can be also considered as a means to enrich membranes in some selected lipid.
Pistolesi
R
R
Istituto di Biochimica e Chimica Medica, Perugia, Italy.
Corazzi
L
L
Arienti
G
G
D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000648:ultrastructure; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000378:metabolism; D008081:Liposomes; D008297:Male; D008561:Membrane Fusion; D008565:Membrane Proteins / Q000502:physiology*; D008861:Microsomes / Q000378:metabolism*; D011804:Quinolines; D051381:Rats; D017207:Rats, Sprague-Dawley; D014302:Trinitrobenzenesulfonic Acid
D008081:Liposomes; D008565:Membrane Proteins; D011804:Quinolines; C000470:EEDQ; D014302:Trinitrobenzenesulfonic Acid