title: Acute erosions of the gastric mucosa in burned rats: effect of gastric acidity and fluid replacement.
abstract: Early changes in the morphology of the gastric mucosa after the skin had been burned were studied using a standardised model in rats. A full thickness burn was inflicted by exposing about 20% of the total body surface area to hot water (99 degrees C) for 10 s. Intragastric acidity was kept at pH 1.0 or pH 7.4 in six experimental groups of eight rats. Rats were subjected to burns with the stomach irrigated at pH 1.0 or pH 7.4. Parallel groups received fluid replacement with a solution of human albumin, and two uninjured groups served as controls. Lesions of the gastric mucosa were measured by planimetry of photographs, and light microscopy was used for histological examination. At an intragastric pH of 1.0, the burned rats developed mucosal erosions covering an average of 13% of the total glandular mucosa; the remaining groups had only minimal mucosal lesions. Erosions of the gastric mucosa after the skin had been burned could be prevented in two ways--either by establishing an alkaline (pH 7.4) milieu in the gastric lumen, or by replacing sufficient fluid to maintain aortic blood pressure at the pre-experiment level. Fluid replacement prevented mucosal erosions even if the intragastric pH was kept at 1.0. Thus both luminal acidity and local tissue blood flow are possible mechanisms for gastric epithelial damage following burns of the skin.
authors: Surgical Research Laboratory, University of Bergen, Norway.
authors: K C
authors: KC
authors: Skolleborg
authors: J E
authors: JE
authors: Grønbech
authors: F E
authors: FE
authors: Abyholm
authors: K
authors: K
authors: Svanes
authors: J
authors: J
authors: Lekven
mesh_terms: D000208:Acute Disease; D000818:Animals; D002056:Burns / Q000473:pathology* / Q000628:therapy; D005440:Fluid Therapy; D005745:Gastric Acidity Determination; D005753:Gastric Mucosa / Q000473:pathology*; D006863:Hydrogen-Ion Concentration; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013047:Specific Pathogen-Free Organisms