title: Significance of FCM-DNA measurement in detecting minimal residual disease in leukemia.
abstract: Using flow cytometry (FCM), we measured the DNA content of bone marrow from 25 patients with acute leukemia (AL) in complete remission (CR) and followed them up for 0.5 to 1 year. Five of 13 patients in early relapse had normal DNA index (DI), while the remaining 8 showed DNA aneuploidy 1.5 to 5 months (mean 2.6 months) before relapse. The relapse rate was higher in CR patients with DNA aneuploidy (88.9%) than in those with normal DI (31.3%, P less than 0.05). Intensive chemotherapy was helpful to prolong CR duration in CR patients with DNA aneuploidy. DNA measurement by FCM was considered a reliable method for detecting minimal residual disease (MRD), and the appearance of DNA aneuploidy might predict a relapse of CR.
authors: Research Laboratory of Hematology, Second Affiliated Hospital, Hebei Medical College, Shijiazhuang.
authors: R X
authors: RX
authors: Zhang
authors: E G
authors: EG
authors: Yao
mesh_terms: D000782:Aneuploidy; D001853:Bone Marrow / Q000737:chemistry; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis* / Q000235:genetics; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis* / Q000235:genetics; D012008:Recurrence
chemical_list: D004273:DNA, Neoplasm