Color-monitored solid-phase multiple peptide synthesis under low-pressure continuous-flow conditions.
Exemplified on fifty peptides derived from proteins of the human immunodeficiency virus (HIV), we summarize our experiences with rapid solid-phase multiple peptide synthesis under low-pressure continuous-flow conditions on standard polystyrene-based resin in a series of concatenated flow reactors with adjustable volume. Alternatively, multiple batchwise synthesis in plastic syringes may be used as a still simpler but a more elaborate procedure. Completeness of acylation of resin-bound free amino groups was monitored by the acid-base indicator bromophenol blue. Efficiency of the coupling reaction was increased by exposure to elevated temperature in an ultrasonic bath. Up to 100 mg of crude peptides were purified on a mass-overloaded Vydac C18 reversed-phase semipreparative HPLC column in one run, this procedure ensuring high recovery of homogeneous peptides. The peptides synthesized were serologically relevant to the detection of anti-HIV-1 antibodies in positive sera.
Krchnák
V
V
Research Institute for Feed Supplements and Veterinary Drugs, Prague, Czechoslovakia.
Vágner
J
J
D000215:Acylation; D000595:Amino Acid Sequence; D000941:Antigens; D002851:Chromatography, High Pressure Liquid; D004864:Equipment and Supplies; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000276:immunology / Q000302:isolation & purification; D011312:Pressure; D013594:Syringes; D014465:Ultrasonics
D000941:Antigens; D010455:Peptides