Effect of 4-cholesten-3-one on lecithin-cholesterol acyltransferase activity and the lipid concentration in the serum of normocholesterolaemic and hypercholesterolaemic rats.
Male Wistar strain rats and PHHC (Prague hereditary hypercholesterolaemic) rats received an intraperitoneal injection of 4-cholesten-3-one for five days. Lecithin-cholesterol acyltransferase activity and total cholesterol, triglyceride and phospholipid levels were determined in their serum. A significant drop in the total cholesterol level was found in normocholesterolaemic Wistar rats after the administration of cholestenone. The serum triglyceride content remained unaltered and the phospholipid concentration showed a downward trend. Lecithin-cholesterol acyltransferase activity was also significantly reduced. In PHHC rats, no significant changes occurred in total cholesterol, triglyceride and phospholipid levels, or in lecithin-cholesterol acyltransferase activity, after the administration of 4-cholesten-3-one. A comparison of serum 4-cholesten-3-one concentrations in the two groups of experimental animals shows that the turnover time for this substance in hypercholesterolaemic rats is only half as long as in normocholesterolaemic rats.
Tallová
J
J
Department of Medical Chemistry and Biochemistry, Faculty of Medicine, Purkynĕ University, Brno.
Dobiásová
M
M
Stárka
L
L
D000818:Animals; D002782:Cholestenes / Q000494:pharmacology*; D002783:Cholestenones / Q000494:pharmacology*; D002784:Cholesterol / Q000097:blood*; D006937:Hypercholesterolemia / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D008055:Lipids / Q000097:blood*; D008297:Male; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000097:blood
D002782:Cholestenes; D002783:Cholestenones; D008055:Lipids; D014280:Triglycerides; C000475:cholest-4-en-3-one; D002784:Cholesterol; D007862:Phosphatidylcholine-Sterol O-Acyltransferase