"In vitro" fibrinolytic activity of a new low molecular weight heparan sulfate.
The fibrinolytic activity, effect on the fibrinolytic activity of plasmin, anticoagulant activity and anti platelet aggregation activity of a low molecular weight heparan sulfate (LMW HS Bal) were investigated "in vitro" on blood plasma obtained from rats and rabbits. LMW HS Bal at concentrations as low as 0.25-2 micrograms/ml prevented thrombin-induced platelet aggregation. At concentrations 25 to 50 times larger it showed no significant anticoagulant activity but a marked fibrinolytic effect. At still larger concentrations LMW HS Bal also potentiated the fibrinolytic activity of plasmin. Conversely, unfractionated heparan sulfate (UHS) at concentrations of 25 to 50 micrograms/ml, only showed anticoagulant activity with no fibrinolytic activity.
Gervasi
G B
GB
Research Laboratories, Baldacci S.p.A., Pisa, Italy.
Farina
C
C
Catalani
R
R
Bartoli
C
C
Baldacci
M
M
D000818:Animals; D004357:Drug Synergism; D005341:Fibrinolysin / Q000494:pharmacology; D005343:Fibrinolytic Agents; D006497:Heparan Sulfate / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D008970:Molecular Weight; D010314:Partial Thromboplastin Time; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains
D005341:Fibrinolysin; D005343:Fibrinolytic Agents; D006497:Heparan Sulfate; D010975:Platelet Aggregation Inhibitors