Immunochemical and structural analysis of the cell wall mannan as the basis of the taxonomic reidentification of a yeast strain.
The immunochemical properties and structural features of D-mannans from two Candida parapsilosis strains were studied. Weak cross-reactivity of D-mannans with antisera produced to C. parapsilosis strains was observed, as well as significant differences in the mannans structures were found by means of methylation analysis, acetolysis and 1H-NMR spectroscopy. In view of the discrepancies found, new taxonomic identification of one strain was carried out. On the basis of assimilation of potassium nitrate and formation of hat-shaped spores as well as other differences found by investigation of physiological characteristics, one strain of C. parapsilosis was reidentified as Hansenula anomala. This work demonstrates that immunochemical and structural investigations of cell-wall polysaccharide components can serve as a basis for taxonomic identification of yeast strains.
Pavliak
V
V
Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Kogan
G
G
Sláviková
E
E
Sandula
J
J
Masler
L
L
D002175:Candida / Q000032:analysis / Q000145:classification* / Q000648:ultrastructure; D002473:Cell Wall / Q000737:chemistry; D002850:Chromatography, Gel; D003429:Cross Reactions; D005779:Immunodiffusion; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000737:chemistry* / Q000276:immunology; D008745:Methylation; D008968:Molecular Conformation; D011233:Precipitin Tests
D008351:Mannans