[The characteristics of the transformed phenotype and the expression of indicator plasmids in the cells of rat embryonic fibroblasts immortalized by oncogene E1Aad5 and transformed by oncogenes E1Aad5+c-Ha-ras].
Rat embryonal fibroblasts of the second passage have been immortalized or transformed with the E1A region of the human adenovirus type 5 and the cloned c-Ha-ras oncogene. Several cell lines obtained greatly differ according to the criteria of the transformed phenotype: saturation cell density, the ability to be cloned on a substrate and in soft agar, the ability to give tumors in nude mice. We have transiently transfected these immortalized and transformed cell lines with CAT-plasmids containing the CAT gene under the control of promoters of the "cell-cycle dependent" genes (human hsp70 and protooncogene c-fos). The results showed that the expression of hsp-CAT and fos-CAT plasmids differed in great extent depending on whether the cells were immortalized (E1Aad5) or transformed (E1Aad5+Ha-ras). The role of cellular genes and cellular transcription factors influencing the expression of transiently transfected CAT-plasmids has been discussed.
Pospelova
T V
TV
Kisliakova
T V
TV
Medvedev
A V
AV
Svetlikova
S B
SB
Pospelov
V A
VA
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002472:Cell Transformation, Viral / Q000235:genetics*; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000166:cytology; D015870:Gene Expression / Q000235:genetics*; D011905:Genes, ras / Q000235:genetics*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D015513:Oncogene Proteins / Q000494:pharmacology; D009857:Oncogenes / Q000235:genetics*; D010641:Phenotype; D010957:Plasmids / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D015533:Transcriptional Activation / Q000187:drug effects / Q000235:genetics; D014162:Transfection / Q000235:genetics
D015513:Oncogene Proteins
Kharakteristika transformirovannog fenotipa i ékspressiia indikatornykh plazmid v kletkakh émbrional'nykh fibroblastov krysy, immortalizovannykh onkogenom E1Aad5 i transformirovannykh onkogenami E1Aad5+c-Ha-ras.