Thermic and pH modulation of phosphofructokinase during trout (Salmo gairdneri R.) haemopoiesis: influence of fructose 6-phosphate.
Thermic and pH modulation of phosphofructokinase (EC 2.7.1.11) activity with respect to fructose 6-phosphate has been studied comparatively in trout (Salmo gairdneri R.) haemopoietic cells and erythrocytes. Phosphofructokinase of both cellular populations displays a biphasic kinetic behaviour with respect to fructose 6-phosphate at two values of pH and temperature. In haemopoietic cells, when pH decreases the enzyme-substrate affinity increase while an opposite effect is found in erythrocytes. Decreases in temperature act as a positive modulator in haemopoietic cells while in erythrocytes this effect is observed only at low fructose 6-phosphate concentrations. Therefore a different pH and temperature modulation of phosphofructokinase during trout haemopoiesis has been established.
Gaitan
S
S
Departamento de Bioquímica y Biología Molecular IV, Facultad de Veterinaria, Universidad Compultense, Madrid, Spain.
Ruiz-Amil
M
M
Tejero
C
C
D000818:Animals; D002454:Cell Differentiation; D004912:Erythrocytes / Q000378:metabolism; D005636:Fructosephosphates / Q000378:metabolism*; D006410:Hematopoiesis / Q000502:physiology*; D006413:Hematopoietic System / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010732:Phosphofructokinase-1 / Q000378:metabolism*; D013696:Temperature; D014337:Trout
D005636:Fructosephosphates; C027618:fructose-6-phosphate; D010732:Phosphofructokinase-1