Clinical evaluation of amperometric enzyme electrodes in continuous-flow analysis for glucose in undiluted whole blood.
We have evaluated the carbon-paste enzyme/wall-jet electrode in continuous-flow analysis for glucose in undiluted whole blood. Responses of the electrode to protein-based and aqueous samples under wall-jet configuration are described. Imprecision in the flow-injection mode was less than 2% for glucose values in the usual analytical range (1-25 mmol/L). The dynamic range for glucose was from 0.03 to 45 mmol/L, with about 100% recovery of added substrate. The performance of the electrode (y) in flow-injection and steady-state modes was compared with a colorimetric method (x; Kodak Ektachem XR700), yielding linear-regression equations of y = 1.152x + 0.295 and y = 0.979x + 0.500, respectively. Among the anticoagulants, blood preservatives, and interfering substances investigated, only ascorbic acid gave a significant positive bias. The electrode exhibited good stability for analyses of undiluted whole-blood samples.
Gunasingham
H
H
Department of Chemistry, National University of Singapore.
Tan
C H
CH
Aw
T C
TC
D001322:Autoanalysis / Q000295:instrumentation; D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis*; D003124:Colorimetry; D004563:Electrochemistry; D006801:Humans; D012212:Rheology
D001786:Blood Glucose