Immunochemical determination of lipoprotein Lp(a): comparison of Laurell electrophoresis and ELISA.
The lipoprotein Lp(a) concentrations in serum from 520 persons (317 men and 203 women) were measured by two different Laurell electrophoresis assays (one in house and one commercial) and by an ELISA technique, using polyclonal antibodies from two different animal species. The following results were obtained. 1. The two Laurell techniques gave similar results. The mean values of all three methods were comparable, whereas median values obtained by ELISA were markedly lower (0.85, 0.97 and 0.057 g/l for the two Laurell assays and the ELISA, respectively). 2. All three methods correlated very well with a correlation coefficient of greater than 0.95. 3. Women had significantly higher values than men (p less than 0.01). It was concluded that Lp(a) serum concentrations in the 'pathological range' could be measured with high enough precision by Laurell electrophoresis and by ELISA using polyclonal antibodies from different animal species.
Kostner
G M
GM
Institute of Medical Biochemistry, University of Graz, Austria.
Gries
A
A
Pometta
M
M
Molinari
E
E
Pichler
P
P
Aicher
H
H
D004586:Electrophoresis / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D017270:Lipoprotein(a); D008074:Lipoproteins / Q000097:blood*; D008297:Male; D012044:Regression Analysis
D017270:Lipoprotein(a); D008074:Lipoproteins