Assignment of paramagnetically shifted resonances in the 1H NMR spectrum of horse ferricytochrome c.
The proton resonances of the heme, the axial ligands, and other hyperfine-shifted resonances in the 1H nuclear magnetic resonance spectrum of horse ferricytochrome c have been investigated by means of one- and two-dimensional nuclear Overhauser and magnetization transfer methods. Conditions for saturation transfer experiments in mixtures of ferro- and ferricytochrome c were optimized for the cross assignment of corresponding resonances in the two oxidation states. New resonance assignments were obtained for the methine protons of both thioether bridges, the beta and gamma meso protons, the propionate six heme substituent, the N pi H of His-18, and the Tyr-67 OH. In addition, several recently reported assignments were confirmed. All of the resolved hyperfine-shifted resonances in the spectrum of ferricytochrome c are now identified. The Fermi contact shifts experienced by the heme and ligand protons are discussed.
Feng
Y Q
YQ
Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia 19104-6059.
Roder
H
H
Englander
S W
SW
D000818:Animals; D003574:Cytochrome c Group / Q000378:metabolism*; D006736:Horses; D006859:Hydrogen; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009206:Myocardium / Q000378:metabolism; D011487:Protein Conformation
D003574:Cytochrome c Group; D006859:Hydrogen