Characterization of an Escherichia coli mutant pleiotropically altered in membrane-bound oxidoreductase activities.
An Escherichia coli mutant pleiotropically altered in membrane-bound oxidoreductase activities was isolated following nitrosoguanidine treatment. Mutant R23 was able to grow on glucose, but was unable to grow on succinate or other oxidizable substrates as a sole energy source. Isolated membranes prepared from R23 failed to oxidize succinate and formate; while NADH was oxidized at a reduced rate by membranes. The mutant also exhibited markedly reduced cytochrome content, but normal DL-lactate PMS reductase and H(+)-translocating ATPase activities relative to the parent strain. Bacteriophage Plkc was used to transduce R23 to growth on glycerol, DL-lactate or succinate; regardless of the selection procedure, each of the 179 transductants had gained the ability to grow on all three substrates. The suc- mutation in R23 appeared to be responsible for the loss of growth on oxidizable substrates, altered membrane-bound oxidoreductase activities, resistance to neomycin, and reduced levels of cytochrome components. The suc- mutation was localized in the 6 to 6.5 min region of the E. coli chromosome map utilizing episomal transfers.
Cox
J C
JC
Department of Biology, University of Houston, Texas 77204-513.
Goldschmidt
E P
EP
Jurtshuk
P
P
D002874:Chromosome Mapping; D003580:Cytochromes / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D005560:Formate Dehydrogenases / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D008773:Methylphenazonium Methosulfate / Q000378:metabolism; D009154:Mutation; D009243:NAD / Q000378:metabolism; D009604:Nitrosoguanidines / Q000494:pharmacology; D010088:Oxidoreductases / Q000378:metabolism*; D010641:Phenotype; D006180:Proton-Translocating ATPases / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid; D014161:Transduction, Genetic
D003580:Cytochromes; D008565:Membrane Proteins; D009604:Nitrosoguanidines; D013386:Succinates; D009243:NAD; D008773:Methylphenazonium Methosulfate; D019802:Succinic Acid; D010088:Oxidoreductases; D007770:L-Lactate Dehydrogenase; D005560:Formate Dehydrogenases; D013385:Succinate Dehydrogenase; D006180:Proton-Translocating ATPases