Reduced HLA-A,B,C expression in tumourigenic v-raf transfected human urothelial cells.
Tumourigenic (TGrIII) human urothelial cells grown in vitro have previously been demonstrated to have a markedly decreased expression of beta 2-microglobulin and HLA-A,B,C antigens as compared to non-tumourigenic (TGrII) human urothelial cell lines. Furthermore, during 'spontaneous' in vitro transformation of a non-tumourigenic (TGrII) human urothelial cell line Hu609 into a tumourigenic (TGrIII) subline Hu609T/LLH, changes in morphology and tumourigenicity have been demonstrated to be accompanied by a decreased HLA-A,B,C expression. After malignant transformation of the non-tumourigenic (TGrII) human urothelial cell line HCV29 by DNA transfection with the v-raf oncogene, four sublines could be isolated. In this study we have investigated these sublines for their expression of membrane bound HLA-A,B,C antigens and provide further evidence that an inverse relationship exists between tumourigenicity and monomorphic HLA-A,B,C expression. Treatment of the cells with recombinant human interferon alpha for 3 days increased the expression of HLA-A,B,C antigens by 50-150% indicating that at least some of the reduced HLA-A,B,C expression could be due to decreased synthesis of HLA-A,B,C antigens. All the transfected cell lines overexpress v-raf and c-myc.
Ottesen
S S
SS
Danish Cancer Society, Copenhagen.
Skouv
J
J
Kieler
J
J
D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000276:immunology; D004848:Epithelium / Q000276:immunology; D015234:HLA-A Antigens / Q000032:analysis*; D015235:HLA-B Antigens / Q000032:analysis*; D015236:HLA-C Antigens / Q000032:analysis*; D006801:Humans; D009857:Oncogenes; D014162:Transfection; D001743:Urinary Bladder / Q000276:immunology*
D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015236:HLA-C Antigens