Evaluation of a synthetic peptide-based assay and a rapid dot-blot recombinant test for detection of antibodies to HIV-1 and HIV-2.
Our objective was to evaluate two new serologic assays for detecting HIV-1 and HIV-2 positive and negative sera. A total of 154 human sera from African countries were tested using a synthetic peptide-based passive hemagglutination assay (PHA) and a rapid dot-blot recombinant test (Test-Pack), both of which are marketed as HIV-1/HIV-2 combination assays. Results indicated that the PHA identified all HIV-1 and HIV-2 positive sera, although some were only weakly reactive. It produced one false positive reaction. The Test-Pack correctly detected all positive and negative sera, although several negative sera produced extremely weak reactions. Both tests detected two HIV-2 positive sera which were non-reactive by HIV-1 screening assays.
Constantine
N T
NT
Division of Virology, U.S. Naval Medical Research Unit No. 3, Cairo, Egypt.
Farag
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MM
Watts
D M
DM
D005069:Evaluation Studies as Topic; D005188:False Negative Reactions; D005189:False Positive Reactions; D015483:HIV Antibodies / Q000302:isolation & purification*; D006386:Hemagglutination Tests; D006801:Humans; D015151:Immunoblotting; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
D015483:HIV Antibodies