A quantitative study of the complexation of cadmium in hemolyzed human erythrocytes by 1H NMR spectroscopy.
The stability of complexes formed by Cd2+ in hemolyzed human erythrocytes was studied by spin-echo 1H NMR spectroscopy. Changes in resonances for the carbon-bonded protons of glutathione (GSH) upon addition of the ethylenediaminetetraacetic acid complex of Cd2+ (Cd(EDTA)2-) and the appearance of resonances for Mg(EDTA)2- indicate that the Cd(EDTA)2- complex dissociates in hemolyzed erythrocytes with the formation of Cd(GSH)x and Mg(EDTA)2- complexes. A semiquantitative estimate of the overall stability constant for the complexation of Cd2+ in hemolyzed erythrocytes was obtained from spin-echo 1H NMR data. The stability constant is consistent with the majority of the Cd2+ in erythrocytes present as Cd(SG)2(2-). A conditional equilibrium constant was also determined for the complexation of Mg2+ by ligands in hemolyzed human erythrocytes.
Kadima
W
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Department of Chemistry, University of Alberta, Edmonton, Canada.
Rabenstein
D L
DL
D002104:Cadmium / Q000097:blood*; D004492:Edetic Acid / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D005978:Glutathione / Q000097:blood; D006461:Hemolysis; D006801:Humans; D008274:Magnesium / Q000097:blood; D009682:Magnetic Resonance Spectroscopy
D002104:Cadmium; D004492:Edetic Acid; D005978:Glutathione; D008274:Magnesium