Gamma/delta T cells and alpha/beta T cells differ in their developmental patterns of receptor expression and modulation requirements.
Measurement of the cell surface levels of gamma/delta (TcR 1) and alpha/beta (TcR 2) T cell receptors in the chicken revealed that thymocyte subpopulations that express these receptor isotypes differ remarkably in their rates of receptor acquisition. Whereas TcR 1 expression was relatively high (greater than 10(4) sites per cell) beginning on day 12 of embryonic life, the initial levels of TcR 2 expression on embryonic thymocytes were relatively low (approximately 10(3) sites per cell) when first measurable on day 15, and increased gradually as a function of T cell maturation. In peripheral tissues, the TcR 1 cells also expressed higher receptor levels than did the TcR 2 cells, but the difference was only 2-3-fold. The TcR 2 receptors on immature T cells could be easily modulated by receptor cross-linkage, very much like immunoglobulin receptors on immature B cells. While the TcR 2 receptor modulation occurred within minutes, TcR 1 receptor modulation required several hours for completion, even in the embryonic thymus. The data indicate very different developmental programs for TcR 1 and TcR 2 expression, and suggest fundamental differences in clonal selection modes for the two T cell subpopulations.
George
J F
JF
Division of Developmental and Clinical Immunology, University of Alabama, Birmingham 35294.
Cooper
M D
MD
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002454:Cell Differentiation; D002642:Chick Embryo; D002645:Chickens; D005455:Fluorescent Antibody Technique; D016130:Immunophenotyping; D007422:Intestines / Q000166:cytology; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D013154:Spleen / Q000166:cytology; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology
D000911:Antibodies, Monoclonal; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta