title: Metabolic activity is necessary for activation of T suppressor cells by B cells.
abstract: Ag-primed B cells must express cell-surface IgM, but not IgD or Ia Ag, and must remain metabolically active, in order to activate suppressor T cells (Ts) specific for type III pneumococcal polysaccharide. Ag-primed B cells that were gamma-irradiated with 1000r, or less, retained the ability to activate Ts; however, Ag-primed B cells exposed to UV light were not able to do so. gamma-Irradiated and UV-treated Ag-primed B cells both expressed comparable levels of cell-surface IgM, and both localized to the spleen after in vivo transfer; neither could proliferate in vitro in response to mitogens. By contrast, gamma-irradiated primed B cells were still able to synthesize proteins, whereas UV-treated primed B cells could not. These findings suggest that in order for Ag-primed B cells to activate Ts, they must a) express cell-associated IgM (sIgM) antibody bearing the idiotypic determinants of antibody specific for type III pneumococcal polysaccharide, and b) be able to synthesize protein for either the continued expression of sIgM after cell transfer, or for the elaboration of another protein molecule that is also required for the activation of Ts; this molecule does not appear to be Ia Ag.
authors: Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852.
authors: K L
authors: KL
authors: Elkins
authors: P W
authors: PW
authors: Stashak
authors: P J
authors: PJ
authors: Baker
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D005720:Gamma Rays; D007075:Immunoglobulin M / Q000502:physiology; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D011135:Polysaccharides, Bacterial / Q000276:immunology; D014176:Protein Biosynthesis; D011947:Receptors, Antigen, B-Cell / Q000502:physiology; D013296:Streptococcus pneumoniae / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D014466:Ultraviolet Rays
chemical_list: D007075:Immunoglobulin M; D011135:Polysaccharides, Bacterial; D011947:Receptors, Antigen, B-Cell