Induction of mouse anti-melanoma cytotoxic and suppressor T cells in vitro by an artificial antigen, GM3-lactone.
We investigated the ability of GM3-lactone liposomes to induce anti-melanoma T cell responses in mice. GM3-lactone liposomes, like murine B16 melanoma cells, induced anti-melanoma cytotoxic T cells (CTL) and also suppressor T cells (Ts). A small dose of GM3-lactone (0.0003 micrograms/ml) was enough to generate CTL in the in vitro primary response, whereas relatively large amounts of the antigen (0.03-0.3 microgram/ml) were required for anti-melanoma Ts induction. As the epitope for anti-melanoma Ts is NeuAc but not NeuGc residue on GM3, and anti-melanoma CTL are effectively induced by either GM3(NeuAc) or GM3(NeuGc)-lactone liposomes, GM3(NeuGc)-lactone or GM3(NeuGc) liposomes have potent activity as an artificial melanoma antigen to induce anti-melanoma CTL in vitro.
Harada
Y
Y
Division of Molecular Immunology, School of Medicine, Chiba University.
Sakatsume
M
M
Taniguchi
M
M
D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology; D002240:Carbohydrate Sequence; D005679:G(M3) Ganglioside / Q000031:analogs & derivatives / Q000276:immunology / Q000494:pharmacology*; D005732:Gangliosides / Q000494:pharmacology*; D008081:Liposomes; D008545:Melanoma / Q000097:blood / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D000951:Antigens, Neoplasm; D005679:G(M3) Ganglioside; D005732:Gangliosides; D008081:Liposomes; C047143:ganglioside GM3 lactone