Quinolinate phosphoribosyl transferase is not the oxygen-sensitive site of nicotinamide adenine dinucleotide biosynthesis.
Quinolinate phosphoribosyl transferase (QPT) activity was not affected when Escherichia coli were treated with hyperbaric oxygen. This result is not in accord with a previous report (Biochem. Biophys. Res. Comm. 91:982-990; 1979) in which the enzyme was shown to be rapidly inactivated in E. Coli exposed to 4.2 atmospheres of oxygen. Our data rule out QPT as a site of oxygen toxicity and suggest other mechanisms for the inhibitory effects of the hyperbaric oxygen on pyridine nucleotide biosynthesis.
Gardner
P R
PR
Department of Biochemistry, Duke University Medical Center, Durham, NC 27710.
Fridovich
I
I
D001426:Bacterial Proteins / Q000378:metabolism; D002854:Chromatography, Paper; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology*; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006931:Hyperbaric Oxygenation; D009243:NAD / Q000096:biosynthesis*; D009525:Niacin / Q000378:metabolism; D010430:Pentosyltransferases / Q000378:metabolism*; D017378:Quinolinic Acid; D011805:Quinolinic Acids / Q000378:metabolism; D000098553:Nicotinate-Nucleotide Diphosphorylase (Carboxylating)
D001426:Bacterial Proteins; D005609:Free Radicals; D011805:Quinolinic Acids; D009243:NAD; D009525:Niacin; D006861:Hydrogen Peroxide; D010430:Pentosyltransferases; D000098553:Nicotinate-Nucleotide Diphosphorylase (Carboxylating); D017378:Quinolinic Acid