title: Normal versus sickle red blood cells: hemodynamic and permeability characteristics in reperfusion lung injury.
abstract: Decreased deformability and increased internal viscosity of the sickle red blood cell (SRBC) contribute to abnormal flow in the microcirculation. Since the lungs are commonly affected in sickle cell disease, we compared the hemodynamics of the normal human red blood cell (NRBC) with the SRBC in the pulmonary circulation. The SRBC has decreased antioxidant enzyme activities compared with the NRBC. Thus, using the capillary filtration coefficient (Kfc), we determined the ability of the NRBC and the SRBC to attenuate the increased permeability and resulting edema seen in the oxidant stress of reperfusion lung injury (RLI). We found that lungs perfused with a 5% SRBC perfusate had higher pulmonary arterial pressures (Ppa) and resistances than lungs perfused with a 5% NRBC perfusate. Lungs made ischemic and reperfused with a physiologic cell-free perfusate resulted in a significant increase (P less than .05) in Kfc compared with the preischemic Kfc (.45 +/- .06 to 1.4 +/- 22 mL.min-1.cm H2O.100 g-1). In lungs reperfused with 5% RBC-containing perfusates, the Kfc did not change from preischemic Kfc with NRBCs and decreased from the preischemic Kfc with SRBCs. These findings suggest that the SRBC causes physiologically significant increases in Ppa and resistances and the SRBC, like the NRBC, offers apparent protection in RLI.
authors: Department of Medicine, University of South Alabama College of Medicine, Mobile 36688.
authors: J
authors: J
authors: Haynes
authors: A
authors: A
authors: Seibert
authors: A
authors: A
authors: Shah
authors: A
authors: A
authors: Taylor
mesh_terms: D000755:Anemia, Sickle Cell / Q000097:blood*; D000818:Animals; D001809:Blood Viscosity; D002199:Capillary Permeability; D004907:Erythrocyte Deformability; D004913:Erythrocytes, Abnormal / Q000502:physiology*; D006439:Hemodynamics; D006801:Humans; D066298:In Vitro Techniques; D008168:Lung / Q000098:blood supply*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D015427:Reperfusion Injury / Q000097:blood / Q000503:physiopathology*