title: Possibility to improve preservation of whole blood by leukocyte-depletion before storage.
abstract: In order to evaluate the influence of leukocytes in whole blood on the standard blood bank conditions for storage, we compared several parameters of red cells, leukocytes and platelets in leukocyte-rich and leukocyte-poor whole blood over a storage period of 5 weeks. The leukocyte-depleted units were prepared by filtration with a leukocyte trapping filter (Sepacell R500A). Our data indicate improved storage conditions, made evident by significantly lower cell damage: smaller increases of elastase, beta-thromboglobulin and lactate dehydrogenase in leukocyte-depleted blood. This improvement was achieved in a simple two-bag system using only filtration, without addition of rejuvenating solutions. In addition, this procedure allows greater flexibility in the use of filtrated blood for transfusions.
authors: Department of Medicine III, University of Munich, FRG.
authors: C
authors: C
authors: Riedner
authors: M U
authors: MU
authors: Heim
authors: W
authors: W
authors: Mempel
authors: W
authors: W
authors: Wilmanns
mesh_terms: D000255:Adenosine Triphosphate / Q000097:blood; D001793:Blood Preservation / Q000379:methods*; D002469:Cell Separation; D015926:Complement C3a / Q000378:metabolism; D004163:Diphosphoglyceric Acids / Q000097:blood; D004906:Erythrocyte Count; D005344:Fibrinopeptide A / Q000378:metabolism; D005374:Filtration; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007958:Leukocyte Count; D007962:Leukocytes; D010196:Pancreatic Elastase / Q000097:blood; D010976:Platelet Count; D001620:beta-Thromboglobulin / Q000378:metabolism
chemical_list: D004163:Diphosphoglyceric Acids; D001620:beta-Thromboglobulin; D005344:Fibrinopeptide A; D015926:Complement C3a; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase; D010196:Pancreatic Elastase