The role of beta 2-microglobulin in peptide binding by class I molecules.
Efficient transport of class I major histocompatibility complex molecules to the cell surface requires association of the class I heavy chain with endogenous peptide and the class I light chain, beta 2-microglobulin (beta 2M). A mutant cell line deficient in beta 2M transports low amounts of nonpeptide-associated heavy chains to the cell surface that can associate with exogenously provided beta 2M and synthetic peptide antigens. Normal beta 2M-sufficient cells grown in serum-free media devoid of beta 2M also require an exogenous source of beta 2M to efficiently bind synthetic peptide. Thus, class I molecules on normal cells do not spontaneously bind or exchange peptides.
Vitiello
A
A
Cytel Corporation, La Jolla, CA 92037.
Potter
T A
TA
Sherman
L A
LA
D000011:Abelson murine leukemia virus / Q000235:genetics; D000818:Animals; D000906:Antibodies; D001692:Biological Transport; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D002471:Cell Transformation, Neoplastic; D003602:Cytotoxicity, Immunologic; D015395:Histocompatibility Antigens Class I / Q000032:analysis / Q000378:metabolism*; D007143:Immunoglobulin Heavy Chains / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D008815:Mice, Inbred Strains; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D001613:beta 2-Microglobulin / Q000235:genetics / Q000378:metabolism*
D000906:Antibodies; D015395:Histocompatibility Antigens Class I; D007143:Immunoglobulin Heavy Chains; D001613:beta 2-Microglobulin