The insulin-like effects of selenate in rat adipocytes.
Selenate was found to have several insulin-like effects in rat adipocytes: stimulation of glucose transport activity by translocation of two types of glucose transporters from intracellular sites to the plasma membrane, stimulation of cAMP phosphodiesterase activity, and stimulation of ribosomal S6 protein phosphorylation. Furthermore, in intact cells addition of 1 mM selenate stimulated tyrosyl phosphorylation of 210-, 170-, 120-, 95-, 70-, and 60-kDa proteins but failed to stimulate insulin receptor kinase activity, suggesting that selenate stimulated other tyrosine kinase. In the presence of insulin, selenate enhances insulin receptor kinase activity and phosphorylations of insulin-stimulated tyrosyl phosphoproteins. These results may provide clues for the elucidation of the role of selenium in animals and the mechanism of insulin action.
Ezaki
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Division of Nutrition and Diseases, National Institute of Nutrition, Tokyo, Japan.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects*; D000818:Animals; D001345:Autoradiography; D002474:Cell-Free System; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005947:Glucose / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D010766:Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism; D017027:Protein Tyrosine Phosphatases; D051381:Rats; D011919:Rats, Inbred Strains; D011972:Receptor, Insulin / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism; D064586:Selenic Acid; D012643:Selenium / Q000494:pharmacology*; D018036:Selenium Compounds
D007328:Insulin; D012269:Ribosomal Proteins; D018036:Selenium Compounds; D010770:Phosphotransferases; D011972:Receptor, Insulin; D010749:Phosphoprotein Phosphatases; D017027:Protein Tyrosine Phosphatases; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D012643:Selenium; D064586:Selenic Acid; D005947:Glucose