In vivo effect of 2-deoxy-D-glucose on glucose-6-phosphate dehydrogenase activity in the cytosol of liver, heart and skeletal muscle of rats.
2-deoxy-D-glucose (2-DG), the unmetabolizable analogue of glucose induces a series of metabolic, hormonal and behavioral responses, causing cellular glucoprivation. According to in vitro studies, 2-DG inhibits phosphofructokinase in cultured human cells. The present investigations deal with changes in the cytosolic glucose-6-phosphate dehydrogenase activity following in vivo 2-DG administration. A single dose of 2-DG (600 mg/kg) has no influence on the activity of glucose-6-phosphate dehydrogenase in the cytosol of liver, heart and skeletal muscle of the rat. The concomitant increase in serum glucose, lactate and FFA concentrations observed in the study indicates indirectly a stimulation of adrenergic system. After three days of successive administration of 2-DG to rats, dehydrogenase activity decreased in the liver by approx 57% and in the skeletal muscle by approx 82% in comparison with control animals. Moreover the in vivo effect of 2-DG was found to be fully reversible, probably when the total amount of the inhibitor was excreted.
Torlińska
T
T
Department of Physiology, Academy of Medicine, Poznań.
Ozegowski
S
S
Paluszak
J
J
Hryniewiecki
T
T
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003600:Cytosol / Q000201:enzymology*; D003847:Deoxyglucose / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D007773:Lactates / Q000097:blood; D008099:Liver / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D009132:Muscles / Q000201:enzymology* / Q000648:ultrastructure; D009206:Myocardium / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains
D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D007773:Lactates; D003847:Deoxyglucose; D005954:Glucosephosphate Dehydrogenase