[A study of development and lesion of dental hard tissue. Quantitative analysis of trace elements (Cd, Zn, Pb and Cu) in different geological areas of Japan].
Trace elements (Cd, Zn, Pb and Cu) levels were determined in lower deciduous incisors which were collected from four areas (Sapporo, Yokosuka, Nagano and Kitakyushu) in Japan. Samples were divided into enamel and dentin, and dissolved with nitric and dried at 80 degrees C. Each element was separated chromatographically using an acetone-HCL gradient solution with a cation exchange resin and then analyzed by atomic absorption spectrophotometry. The results were as follows: 1. Ca, P, Ca/P ratio levels showed no significant differences among four areas. 2. In the enamel, (1) Cd levels of each area were 0.593 micrograms/g (Yokosuka) greater than 0.315 micrograms/g (Sapporo) greater than 0.261 micrograms/g (Nagano) greater than 0.222 micrograms/g (Kitakyushu). (2) Zn levels of each area were 174.912 micrograms/g (Nagano) greater than 157.473 micrograms/g (Yokosuka) greater than 150.971 micrograms/g (Kitakyushu) greater than 128.980 micrograms/g (Sapporo). (3) Pb levels of each area were 8.412 micrograms/g (Kitakyushu) greater than 7.925 micrograms/g (Yokosuka) greater than 5.426 micrograms/g (Sapporo) greater than 4.285 micrograms/g (Nagano). (4) Cu levels of each area were 9.276 micrograms/g (Kitakyushu) greater than 6.928 micrograms/g (Nagano) greater than 6.178 micrograms/g (Yokosuka) greater than 4.389 micrograms/g (Sapporo). 3. In the dentin, (1) Cd levels of each area were 0.214 micrograms/g (Sapporo) greater than 0.184 micrograms/g (Kitakyushu) greater than 0.145 micrograms/g (Nagano) greater than 0.119 micrograms/g (Yokosuka). (2) Zn levels of each area were 124.152 micrograms/g (Yokosuka) greater than 112.242 micrograms/g (Nagano) greater than 111.952 micrograms/g (Sapporo) greater than 111.456 micrograms/g (Kitakyushu). (3) Pb levels of each area were 3.584 micrograms/g (Kitakyushu) greater than 3.019 micrograms/g (Yokosuka) greater than 1.819 micrograms/g (Sapporo) greater than 1.523 micrograms/g (Nagano). (4) Cu levels of each area were 3.734 micrograms/g (Kitakyushu) greater than 2.221 micrograms/g (Nagano) greater than 2.098 micrograms/g (Yokosuka) greater than 1.664 micrograms/g (Sapporo). Therefore, the results suggest that the trace elements levels in deciduous teeth depended on the environmental condition of each area and the deciduous teeth could be a useful material as an environmental contamination indicator.
Tange
T
T
Department of Pedodontics, Kanagawa Dental College.
Shimizu
K
K
Imashiro
S
S
Kumasaka
S
S
Higaki
M
M
D002104:Cadmium / Q000032:analysis; D002648:Child; D002852:Chromatography, Ion Exchange; D003300:Copper / Q000032:analysis; D004784:Environmental Monitoring / Q000379:methods*; D006801:Humans; D007564:Japan; D007854:Lead / Q000032:analysis; D013054:Spectrophotometry, Atomic; D014094:Tooth, Deciduous / Q000737:chemistry*; D014131:Trace Elements / Q000032:analysis*; D015032:Zinc / Q000032:analysis
D014131:Trace Elements; D002104:Cadmium; D007854:Lead; D003300:Copper; D015032:Zinc