Isolation of Mycobacterium paratuberculosis from the uterine flush fluids of cows with clinical paratuberculosis.
Mycobacterium paratuberculosis was isolated from the uterine flush fluids obtained from 3 to 4 cows with clinical paratuberculosis. Four cows with clinical paratuberculosis were subjected to uterine lavage, using an established embryo recovery technique, and the recovered fluids were cultured for M paratuberculosis. Mycobacterium paratuberculosis has previously been demonstrated to adhere to bovine ova. Embryos within the uterus of a superovulated cow infected with M paratuberculosis could be exposed to the organism.
Rohde
R F
RF
Department of Veterinary Preventive Medicine, College of Veterinary Medicine, Ohio State University, Columbus 43210.
Shulaw
W P
WP
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D005260:Female; D005779:Immunodiffusion; D009161:Mycobacterium / Q000302:isolation & purification*; D010283:Paratuberculosis / Q000382:microbiology*; D007507:Therapeutic Irrigation / Q000662:veterinary; D014599:Uterus / Q000382:microbiology*