An automated microtechnique for selenium determination in human body fluids by flow injection hydride atomic absorption spectrometry (FI-HAAS).
The automation of a flow injection system for the hydride generation of selenium and its subsequent determination by atomic absorption spectrometry (FI-HAAS) is described. Pre-treatment of the sample and the details of the automated equipment are reviewed. For the FI-HAAS selenium analysis a volume of 350 microL of acid-digested sample solution is injected. The on-line generated hydride is delivery by the gas-liquid separator and is transported together with an Ar stream to the heated quartz cell for the atomic absorption determination. The absolute detection limit is 35 pg Se; the relative detection limit 0.10 micrograms/L Se. The absolute determination limit in real biological samples is 110 pg Se; the relative detection limit 0.31 micrograms/L Se. The accuracy of the method was evaluated via analysis of certified standard reference materials. Quality control was made by comparing FI-HAAS and instrumental neutron activation analysis (INAA), as an independent analytical method. Two acid-digestion procedures (in open vessels at atmospheric pressure and bomb-digestion in pressure vessels) were experimentally tested. To determine the effectiveness of the selenium reduction and the completeness of the selenium hydride formation a parallel selenium determination was carried out by means of ICP-AES and FI-HAAS analysis. FI-HAAS was applied for blood serum analysis of children undergoing long-term total parenteral nutrition, as well as of persons with high dietary selenium intake, and for human milk analysis.
Negretti de Brätter
V E
VE
Hahn-Meitner-Institut Berlin GmbH, Department of Trace Elements in Health and Nutrition, Fed. Rep. of Germany.
Brätter
P
P
Tomiak
A
A
D001322:Autoanalysis; D002138:Calibration; D002648:Child; D004032:Diet; D017022:Flow Injection Analysis; D006801:Humans; D008895:Milk, Human / Q000737:chemistry*; D009752:Nutritional Status; D010289:Parenteral Nutrition, Total; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D012643:Selenium / Q000008:administration & dosage / Q000032:analysis* / Q000097:blood; D013054:Spectrophotometry, Atomic / Q000379:methods*
D012643:Selenium