The effect of iron and iron chelators on the in-vitro block to development of the mouse preimplantation embryo: BAT6 a new medium for improved culture of mouse embryos in vitro.
The effect of iron and iron chelators on the development of the mouse embryo in vitro from the 1-cell stage to the blastocyst has been investigated. An adverse effect of iron was found. The high affinity iron chelator, desferal, also blocked development, whilst transferrin (whether as apoprotein or saturated with iron), DETAPAC and EDTA promoted development. The addition of transferrin permitted development to the blastocyst stage of embryos from stains normally exhibiting the 2-cell block. Under such circumstances both the rate of embryonic development and the proportion of embryos reaching the blastocyst stage approached levels found in vivo. Based on these results, a new medium, BAT6, is described for the optimal in-vitro culture of mouse embryos.
Nasr-Esfahani
M
M
Department of Anatomy, Cambridge, UK.
Johnson
M H
MH
Aitken
R J
RJ
D000818:Animals; D001059:Apoproteins / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects*; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods*; D003676:Deferoxamine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000494:pharmacology; D004622:Embryo, Mammalian / Q000502:physiology*; D047108:Embryonic Development; D005260:Female; D005296:Ferrous Compounds / Q000494:pharmacology; D007501:Iron / Q000494:pharmacology*; D007502:Iron Chelating Agents / Q000494:pharmacology*; D051379:Mice; D004369:Pentetic Acid / Q000494:pharmacology; D011247:Pregnancy; D014168:Transferrin / Q000494:pharmacology
D001059:Apoproteins; D003470:Culture Media; D005296:Ferrous Compounds; D007502:Iron Chelating Agents; D014168:Transferrin; C028284:apotransferrin; C020748:ferrous sulfate; D004369:Pentetic Acid; D004492:Edetic Acid; D007501:Iron; D003676:Deferoxamine; D001205:Ascorbic Acid