title: Cystic fibrosis marker testing in Bohemia with polymerase chain reaction.
abstract: Conditions for assessing KM-19 probe detected by Pst-1 restriction fragment length polymorphism (RFLP) by means of polymerase chain reaction were provided. Computer controlled mechanical arm with waterbaths and cloned heat-stable DNA polymerase was used. Results of KM-19 allelic frequencies on 90 cystic fibrosis chromosomes are presented. Allele two frequency was -0.833.
authors: I. Institute for Human Genetics, Free University, West Berlin.
authors: M
authors: M
authors: Macek
authors: I
authors: I
authors: Böhm
authors: L
authors: L
authors: Arnold
authors: J
authors: J
authors: Smrt
authors: R
authors: R
authors: Duspivová
authors: V
authors: V
authors: Vávrová
authors: M
authors: M
authors: Macek
authors: E
authors: E
authors: Jelínková
authors: J
authors: J
authors: Schmidtke
mesh_terms: D000483:Alleles; D003550:Cystic Fibrosis / Q000175:diagnosis / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D006801:Humans; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011296:Prenatal Diagnosis
chemical_list: D005819:Genetic Markers; D004247:DNA