Automated liquid chromatographic determination of aflatoxin M1 in milk using on-line dialysis for sample preparation.
A procedure has been developed for the automated isolation of aflatoxin M1 from decreamed milk. The method uses on-line stopped flow dialysis and subsequent trace enrichment on a reverse-phase column. After a back-flush to the analytical liquid chromatography column, aflatoxin M1 is determined with fluorescence detection. Fully automated analysis is possible with reproducible dialysis recoveries above 50% (CV = 7.5%, n = 25 at the 50 ng/kg level) and determination levels of 20 ng/kg within 20 min.
Tuinstra
L G
LG
State Institute for Quality control of Agricultural Products (RIKILT), Wageningen, The Netherlands.
Kienhuis
P G
PG
Dols
P
P
D016607:Aflatoxin M1; D000348:Aflatoxins / Q000032:analysis*; D000818:Animals; D001322:Autoanalysis; D002853:Chromatography, Liquid / Q000379:methods; D003956:Dialysis / Q000379:methods; D005453:Fluorescence; D005506:Food Contamination / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results
D000348:Aflatoxins; D016607:Aflatoxin M1