Effects of PGE2, carbenoxolone, cholecystokinin, and the thromboxane-mimetic U46619 on protein and glycoprotein production by isolated pig gastric mucosal cells.
We studied the effects of prostaglandin (PG) E2, carbenoxolone, cholecystokinin-octapeptide (CCK-OP), and the thromboxane-mimetic U46619, all known to stimulate gastric mucus secretion in vivo, on protein and glycoprotein synthesis in and release from isolated and enriched pig gastric mucous cells, as measured by the incorporation of [3H]L-leucine and N-acetyl-[14C]D-glucosamine respectively into cellular and released acid insoluble material. PGE2 stimulated glycoprotein and protein synthesis (EC50 7 and 30 nmol/L, respectively) and release (EC50 50 and 140 nmol/L, respectively) in a concentration-dependent manner, whereas carbenoxolone, CCK-OP and U46619 failed to enhance the incorporation of the tracers. We conclude that stimulation of mucus secretion by PGE2 is related to direct effects on protein and glycoprotein production of gastric non-parietal cells, whereas indirect effects may be involved in the stimulation by carbenoxolone, CCK-OP, and U46619.
Heim
H K
HK
Department of Pharmacology, Medical School, Hanover, FRG.
Oestmann
A
A
Sewing
K F
KF
D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; D000117:Acetylglucosamine / Q000378:metabolism; D000818:Animals; D002229:Carbenoxolone / Q000494:pharmacology*; D015232:Dinoprostone / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis*; D066298:In Vitro Techniques; D010772:Phosphotungstic Acid; D011450:Prostaglandin Endoperoxides, Synthetic / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012844:Sincalide / Q000494:pharmacology*; D013552:Swine; D014238:Trichloroacetic Acid
D006023:Glycoproteins; D011450:Prostaglandin Endoperoxides, Synthetic; D010772:Phosphotungstic Acid; D014238:Trichloroacetic Acid; D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; D015232:Dinoprostone; D012844:Sincalide; D002229:Carbenoxolone; D000117:Acetylglucosamine