A monoclonal antibody Hy20-54-16-3L to lambda (lambda) light chain of human immunoglobulin reacts with amyloid in Alzheimer's disease brain.
We have previously described a monoclonal antibody Hy20-54-16-3L raised against crude primary (AL) amyloid fibrils that preferentially labelled amyloid deposits in the brains of patients with Alzheimer's disease (AD) (Ishii et al., Neuropathol. Appl. Neurobiol., 12 (1986) 441-445). In the present study, we analyzed the protein from an AL-amyloid laden spleen by gel filtration and SDS-PAGE; a partial amino acid sequence of the Hy20-54-16-3L-immunoreactive band excised from Western blots was determined and found to be a lambda light chain of human immunoglobulins. Additionally we have confirmed that this monoclonal antibody reacts with amyloid in AD brain by using a peroxidase-linked immunoelectron microscope technique. It is concluded that an epitope shared with a lambda light chain is included in or associated with amyloid deposits in the AD brain.
Kametani
F
F
Department of Molecular Biology, Psychiatric Research Institute of Tokyo, Japan.
Tanaka
K
K
Sato
M
M
Haga
S
S
Saitoh
T
T
Shinoda
T
T
Ishii
T
T
D000544:Alzheimer Disease / Q000473:pathology*; D000595:Amino Acid Sequence; D000682:Amyloid / Q000276:immunology; D016229:Amyloid beta-Peptides / Q000032:analysis* / Q000276:immunology; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000302:isolation & purification*; D001921:Brain / Q000473:pathology* / Q000648:ultrastructure; D006801:Humans; D007124:Immunoenzyme Techniques; D007147:Immunoglobulin Light Chains; D007146:Immunoglobulin lambda-Chains; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data
D000682:Amyloid; D016229:Amyloid beta-Peptides; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D007147:Immunoglobulin Light Chains; D007146:Immunoglobulin lambda-Chains