Amine oxidase activities in chemically-induced mammary cancer in the rat.
The activities and distributions of monoamine oxidase (MAO) and semicarbazide-sensitive amine oxidase (SSAO) were studied in solid breast tumours induced in rat by treatment with 7,12-dimethylbenz(alpha)anthracene (DMBA). It was observed that increasing degree of malignancy was associated with an increase of MAO-A activity an decrease of MAO-B and SSAO activities. The Km values did not change significantly with malignancy but Vmax values for MAO-A increased whereas Vmax for MAO-B and SSAO diminished with malignancy. It was detected in the more malignant tumours the presence of endogenous reversible inhibitor of SSAO activity not removed by dialysis.
Lizcano
J M
JM
Department Bioquímica i Biologia Molecular, Facultat de Medicina, Universitat Autónoma de Barcelona, Spain.
Escrich
E
E
Tipton
K F
KF
Unzeta
M
M
D015127:9,10-Dimethyl-1,2-benzanthracene; D006631:Amine Oxidase (Copper-Containing) / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D003010:Clorgyline / Q000494:pharmacology; D005260:Female; D005350:Fibroma / Q000139:chemically induced / Q000201:enzymology*; D007700:Kinetics; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000201:enzymology*; D008995:Monoamine Oxidase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D015127:9,10-Dimethyl-1,2-benzanthracene; D006631:Amine Oxidase (Copper-Containing); D008995:Monoamine Oxidase; D003010:Clorgyline