title: Interferon-gamma production closely related to antibody production in lymphocyte cultures stimulated with mumps virus.
abstract: Enzyme-linked immunosorbent assay (ELISA) antibody to mumps virus and virus specific interferon (IFN)-gamma production were investigated in lymphocyte cultures stimulated with mumps virus before and after immunization with live mumps vaccine. Synthesis of immunoglobulin (Ig) M but not Ig G was enhanced after vaccination. Spontaneous production of mumps ELISA antibodies in lymphocyte culture increased after vaccination and substantially higher levels of antibodies were produced when lymphocytes were stimulated with mumps virus after vaccination. The production of mumps ELISA antibodies was closely related to IFN-gamma production (r = 0.326, p less than 0.01) but not to IFN-alpha production (r = 0.084, p greater than 0.05).
authors: Department of Pediatrics, Keio University, School of Medicine, Japan.
authors: T
authors: T
authors: Nakayama
authors: S
authors: S
authors: Unno
authors: T
authors: T
authors: Urano
authors: M
authors: M
authors: Osano
authors: K
authors: K
authors: Sasaki
authors: S
authors: S
authors: Makino
mesh_terms: D000914:Antibodies, Viral / Q000096:biosynthesis*; D002478:Cells, Cultured; D002675:Child, Preschool; D004338:Drug Combinations; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007223:Infant; D007371:Interferon-gamma / Q000096:biosynthesis*; D008214:Lymphocytes / Q000276:immunology*; D008458:Measles Vaccine / Q000494:pharmacology; D022542:Measles-Mumps-Rubella Vaccine; D009108:Mumps Vaccine / Q000494:pharmacology; D009109:Mumps virus / Q000502:physiology*; D012411:Rubella Vaccine / Q000494:pharmacology; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000494:pharmacology
chemical_list: D000914:Antibodies, Viral; D004338:Drug Combinations; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008458:Measles Vaccine; D022542:Measles-Mumps-Rubella Vaccine; D009108:Mumps Vaccine; D012411:Rubella Vaccine; D014613:Vaccines, Attenuated; D007371:Interferon-gamma