title: Induction of suppressor cell activity by cyclosporin A and/or uremic serum in normal versus uremic peripheral blood mononuclear cells.
abstract: We investigated induction of suppressor cell activity in peripheral blood mononuclear cells (PBMC) from normal or uremic subjects. The cells were primed with cyclosporin A (CS-A), uremic serum, or both and subsequently cocultured with fresh phytohemagglutinin-stimulated normal allogeneic responders. CS-A-primed normal as well as uremic PBMC significantly suppressed responder cell thymidine incorporation. Uremic serum primed normal, but not the uremic, PBMC exerted a significant suppression on responder cell proliferation. No significant additive suppressive effect was detected following coculture of responders with PBMC primed by CS-A and uremic serum together. Exclusion of a distinct suppressor cell subset by panning, using anti-Leu-2b monoclonal antibody, did not result in blunting suppressive activity of the CS-A or uremic serum pretreated cells. On the other hand, adherent cell depletion of primed PBMC completely abolished their subsequent suppressive effect on responder cell proliferation.
authors: Nephrology Division, Assaf Harofeh Medical Center, Tel Aviv University, Zerifin, Israel.
authors: D
authors: D
authors: Modai
authors: S
authors: S
authors: Berman
authors: D
authors: D
authors: Cohen
authors: M
authors: M
authors: Amit
authors: M
authors: M
authors: Cohn
authors: J
authors: J
authors: Weissgarten
authors: Z
authors: Z
authors: Averbukh
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003524:Cyclosporins / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D050378:T-Lymphocytes, Regulatory / Q000166:cytology*; D014511:Uremia / Q000097:blood*
chemical_list: D003524:Cyclosporins