Cystic fibrosis marker testing in Bohemia with polymerase chain reaction.
Conditions for assessing KM-19 probe detected by Pst-1 restriction fragment length polymorphism (RFLP) by means of polymerase chain reaction were provided. Computer controlled mechanical arm with waterbaths and cloned heat-stable DNA polymerase was used. Results of KM-19 allelic frequencies on 90 cystic fibrosis chromosomes are presented. Allele two frequency was -0.833.
Macek
M
M
I. Institute for Human Genetics, Free University, West Berlin.
Böhm
I
I
Arnold
L
L
Smrt
J
J
Duspivová
R
R
Vávrová
V
V
Macek
M
M
Jelínková
E
E
Schmidtke
J
J
D000483:Alleles; D003550:Cystic Fibrosis / Q000175:diagnosis / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D006801:Humans; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011296:Prenatal Diagnosis
D005819:Genetic Markers; D004247:DNA