Molecular cloning and complete nucleotide sequence of the attenuated rabies virus SAD B19.
Complementary DNA spanning the entire genome of the attenuated rabies virus strain SAD B19 which is used for oral immunization of foxes in Europe was cloned and sequenced. The viral genome comprises 11,928 nucleotides and encodes the five viral proteins N, NS, M, G, and L. Deduced protein sequences are highly similar to those of the pathogenic PV strain, homologies ranging from 90.6% for the M to 98.6% for the L protein. The five cistrons are separated by intergenic regions of 2, 5, 5, and 24 nucleotides, respectively. The G transcription stop/polyadenylation consensus signal in SAD B19 is destroyed by a deletion of three A residues. The strong conservation of both noncoding and coding nucleotide sequences indicates a high selective pressure on the primary structure of rabies virus genomic RNA.
Conzelmann
K K
KK
Federal Research Centre for Virus Diseases of Animals, Tübingen, Federal Republic of Germany.
Cox
J H
JH
Schneider
L G
LG
Thiel
H J
HJ
D000595:Amino Acid Sequence; D000818:Animals; D000954:Antigens, Surface / Q000235:genetics; D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D002460:Cell Line; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000235:genetics; D015345:Oligonucleotide Probes; D012367:RNA, Viral / Q000235:genetics*; D011819:Rabies Vaccines / Q000235:genetics*; D011820:Rabies virus / Q000235:genetics*; D012692:Serial Passage; D014613:Vaccines, Attenuated / Q000235:genetics; D014758:Viral Core Proteins / Q000235:genetics; D014763:Viral Matrix Proteins / Q000235:genetics; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins / Q000235:genetics
D000954:Antigens, Surface; D004279:DNA, Viral; D006023:Glycoproteins; D009698:Nucleoproteins; D015345:Oligonucleotide Probes; D012367:RNA, Viral; D011819:Rabies Vaccines; D014613:Vaccines, Attenuated; D014758:Viral Core Proteins; D014763:Viral Matrix Proteins; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins