The amphiphilic action of vasopressin and analogues on the plasma membrane of Amoeba proteus.
Arginine (AVP) and lysine vasopressin induce a weak but statistically significant increase in the water permeability of Amoeba proteus plasmalemma. Vasotocin and deaminovasopressin, which share the hydroosmotic properties of AVP on classical vertebrate systems, are without effects on Amoeba while SKF 101926, a synthetic AVP antagonist, is even more effective than the parent compound. Theophyllin and dibutyryl-cAMP do not affect AVP action on Amoeba. Lithium, oxytocin, and carbachol are also without effect. Thus, it is unlikely that either V2 (cAMP) or V1 (phosphatidylinositol choline) receptors are involved. A clear correlation has been found between the amphiphilic character of tested peptides and their effect on Amoeba water permeability. Classical amphiphilic peptides, melittin, mastoparan, and fragment 1-8 of alpha-neoendorphin, also increased water permeability in Amoeba. It is known that vasopressin can interact with artificial lipid membranes, increasing their permeability to water. We propose that amphiphilic members of the AVP family interact directly with the lipid phase of the Amoeba membrane. Their incorporation within the lipid bilayer may cause local disruptions or may create micellar water channels as shown for other amphiphilic proteins. Our observations provide a model for the early evolution of peptide hormone systems, preceding the appearance of specific membrane receptors and associated second messenger amplifying mechanisms.
Mayers
P
P
Département de Sciences Biologiques, Université de Montréal, Quebec, Canada.
Couillard
P
P
D000656:Amoeba / Q000187:drug effects* / Q000648:ultrastructure; D000818:Animals; D001127:Arginine Vasopressin / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology; D003894:Deamino Arginine Vasopressin / Q000494:pharmacology; D004723:Endorphins / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins; D050356:Lipid Metabolism; D008236:Lypressin / Q000494:pharmacology; D008555:Melitten / Q000494:pharmacology; D010121:Oxytocin / Q000494:pharmacology; D010455:Peptides; D011498:Protein Precursors / Q000494:pharmacology; D011945:Receptors, Angiotensin / Q000187:drug effects / Q000502:physiology; D017483:Receptors, Vasopressin; D014667:Vasopressins / Q000494:pharmacology*; D014862:Wasp Venoms / Q000494:pharmacology; D014867:Water / Q000378:metabolism*
D004723:Endorphins; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D011498:Protein Precursors; D011945:Receptors, Angiotensin; D017483:Receptors, Vasopressin; D014862:Wasp Venoms; D014867:Water; D014667:Vasopressins; D001127:Arginine Vasopressin; D008555:Melitten; D010121:Oxytocin; D008236:Lypressin; C025681:alpha-neoendorphin; C037500:mastoparan; D003894:Deamino Arginine Vasopressin