Evidence for release of prostaglandin F2 alpha in contractile response of guinea pig trachea to norepinephrine.
The concentration (10(-8)-10(-5) M)-dependent contractile response of norepinephrine was completely inhibited by cyclooxygenase inhibitors but not by a thromboxane synthetase inhibitor. The amount of prostaglandin F2 alpha was significantly increased in the presence of norepinephrine (10(-5) M). Norepinephrine increased the amount of prostaglandin D2, although not significantly. The norepinephrine-evoked prostaglandin release was inhibited by tolazoline (3 x 10(-5) M). These results suggest that norepinephrine evoked the release of prostaglandins, mainly prostaglandin F2 alpha to induce the contraction of the guinea pig trachea.
Takayanagi
I
I
Department of Chemical Pharmacology, Toho University School of Pharmaceutical Sciences, Chiba, Japan.
Kawano
K
K
Koike
K
K
D000818:Animals; D016861:Cyclooxygenase Inhibitors; D015237:Dinoprost / Q000378:metabolism*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D009638:Norepinephrine / Q000494:pharmacology*; D014043:Tolazoline / Q000494:pharmacology; D014132:Trachea / Q000187:drug effects
D016861:Cyclooxygenase Inhibitors; D015237:Dinoprost; D014043:Tolazoline; D009638:Norepinephrine