Binding of collagens to an enterotoxigenic strain of Escherichia coli.
An enterotoxigenic strain of Escherichia coli, B34289c, has been shown to bind the N-terminal region of fibronectin with high affinity (G. Fröman, L. M. Switalski, A. Faris, T. Wadström, and M. Höök, J. Biol. Chem. 259:14899-14905, 1984). We now report that this strain also binds collagen. The binding of 125I-labeled type II collagen to bacteria was time dependent and reversible. Bacteria expressed a limited number of collagen receptors (2.2 x 10(4) per cell) and bound collagen with a Kd of 20 nM. All collagen types tested (I to V) as well as all tested cyanogen bromide-generated peptides [alpha 1(I)CB2, alpha 1(I)CB3, alpha 1(I)CB7, alpha 1(I)CB8, and alpha 2(I)CB4] were recognized by bacterial receptors, as demonstrated by the ability of these proteins to inhibit the binding of 125I-labeled collagen to bacteria. Of several unlabeled proteins tested in competition experiments, fibronectin and its N-terminal region strongly inhibited binding of the radiolabeled collagen to E. coli cells. Conversely, collagen competed with an 125I-labeled 28-kilodalton fibronectin fragment for bacterial binding. Collagen bound to bacteria could be displaced by excess amounts of either unlabeled fibronectin or its N-terminal fragment. Similarly, collagen could displace 125I-labeled N-terminal peptide of fibronectin bound to the bacterial cell surface. Bacteria grown at 41 degrees C or in the presence of glucose did not express collagen or fibronectin receptors. These results indicate the presence of specific binding sites for collagen on the surface of E. coli cells and furthermore that the collagen and fibronectin binding sites are located in close proximity, possibly on the same structure.
Visai
L
L
Department of Biochemistry, University of Pavia, Italy.
Speziale
P
P
Bozzini
S
S
D001665:Binding Sites; D003094:Collagen / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D007457:Iodine Radioisotopes; D007797:Laminin / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000032:analysis; D038981:Receptors, Collagen
D005353:Fibronectins; D007457:Iodine Radioisotopes; D007797:Laminin; D011956:Receptors, Cell Surface; D038981:Receptors, Collagen; D003094:Collagen