Identification of organic N-chloramines in vitro in stomach fluid from the rat after chlorination.
Amino acids were identified and quantified in the stomach fluid recovered from animals fasted 8, 24, and 48 h. After in vitro chlorination of stomach fluid, three chloramino acids, N-chloroglycine, either N-chloroleucine or N-chloroisoleucine, and N-chlorophenylalanine, were identified by GC/MS analysis of dansylated methyl ester derivatives. Formation of N-chlorovaline and N-chloroserine was suggested by their HPLC retention times, but their identification could not be confirmed by GC/MS data.
Scully
F E
FE
Department of Chemical Sciences, Old Dominion University, Norfolk, Virginia 23529-0126.
Mazina
K E
KE
Ringhand
H P
HP
Chess
E K
EK
Campbell
J A
JA
Johnson
J D
JD
D000818:Animals; D002700:Chloramines / Q000032:analysis* / Q000378:metabolism; D002713:Chlorine / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D005750:Gastric Juice / Q000737:chemistry*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D002700:Chloramines; D002713:Chlorine