Increased UDP-GlcNAc: alpha-mannoside beta(1----4) N-acetylglucosaminyltransferase activity during chick embryo development.
In vitro assays for the beta-N-acetylglucosaminyltransferases (GlcNAcTase) were performed on crude microsomal fractions prepared from 8-day chick embryo fibroblasts (8-day-CEF) and 16-day chick embryo fibroblasts (16-day-CEF) using [3H]mannose-labeled GlcNAc beta 1----2 Man alpha 1----6 (GlcNAc beta 1----2 Man alpha 1----3) Man beta 1----4 GlcNAc beta 1----4 (Fuc alpha 1----6) GlcNAc-Asn and UDP-GlcNAc as substrates. 8-day-CEF synthesize preferentially triantennary complex type chains, whereas 16-day-CEF produce essentially tetraantennary complex type chains. Furthermore oligosaccharides containing the GlcNAc beta 1----4 Man alpha 1----3 Man sequence represent 90% of the structures found in 16-day-CEF versus 30% in 8-day-CEF, indicating an increase in beta-N-acetylglucosaminyltransferase IV activity during embryo development.
Ogier-Denis
E
E
Laboratoire de Biologie et de Pathologie Moléculaires Glycoprotéines, INSERM U 180, Paris, France.
Bauvy
C
C
Moutsita
R
R
Aubery
M
M
Codogno
P
P
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D002642:Chick Embryo / Q000201:enzymology / Q000254:growth & development*; D005964:Glucosyltransferases / Q000378:metabolism*; D008861:Microsomes / Q000201:enzymology; D008969:Molecular Sequence Data; D013997:Time Factors
C060953:flavonol 2'-O-glucosyltransferase; D005964:Glucosyltransferases