Glycosidic bond cleavage of 5-fluoro-2'-deoxyuridine and 5-fluorouridine by amino acyl-tRNA synthetases.
Lysyl-tRNA synthetase isolated from rat liver cleaves glycosidic bond of 5-Fluorouridine and 5-Fluoro-2'-deoxyuridine to generate 5-Fluorouracil. The generation of 5-Fluorouracil was monitored by cellulose thin layer chromatography and by spectrophotometry. The enzyme was found to be 1.4 fold more efficient in cleaving the glycosidic bond of 5-Fluoro-2'-deoxyuridine than 5-Fluorouridine.
Kumar
A M
AM
Department of Microbiology & Cell Biology, Indian Institute of Science, Bangalore.
Nayak
R
R
D000818:Animals; D002855:Chromatography, Thin Layer; D003593:Cytoplasm / Q000201:enzymology; D005467:Floxuridine / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism; D006027:Glycosides / Q000378:metabolism*; D006868:Hydrolysis; D008099:Liver / Q000201:enzymology; D008250:Lysine-tRNA Ligase / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014529:Uridine / Q000031:analogs & derivatives* / Q000378:metabolism
D006027:Glycosides; D005467:Floxuridine; C001943:5-fluorouridine; D006026:Glycoside Hydrolases; D008250:Lysine-tRNA Ligase; C025034:doxifluridine; D014529:Uridine