1,3-Beta-glucan synthase in cell-free extracts from mycelium and protoplasts of Sclerotium glucanicum.
Conditions for obtaining stable protoplasts from Sclerotium glucanicum and their reversion to hyphal growth were determined. 1,3-beta-Glucan synthase activity was detected in particulate enzyme fractions from mycelium and protoplasts of Scl. glucanicum. UDP-[U-14C]glucose was linearly incorporated into a beta-glucan for about 20 min at 25 degrees C. Optimum pH and temperature values, as well as thermal stabilities of the 1,3-beta-glucan synthase activity, were determined. High concentrations of EDTA were inhibitory. Enzyme activity was stimulated by ATP and GTP. The apparent Km value for UDP-glucose was 0.54 mM. The reaction product was characterized as 1,3-beta-glucan by 13C NMR spectroscopy and hydrolysis products of an exo-1,3-beta-glucanase.
Kottutz
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Institut für Biochemie und Biotechnologie, Technischen Universität Braunschweig, FRG.
Rapp
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D000255:Adenosine Triphosphate / Q000494:pharmacology; D004789:Enzyme Activation; D004795:Enzyme Stability; D005658:Fungi / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D005947:Glucose / Q000378:metabolism; D005964:Glucosyltransferases / Q000737:chemistry* / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008565:Membrane Proteins; D011523:Protoplasts / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology; D029702:Schizosaccharomyces pombe Proteins; D013696:Temperature
D008565:Membrane Proteins; D029702:Schizosaccharomyces pombe Proteins; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D005964:Glucosyltransferases; C020570:1,3-beta-glucan synthase; D005947:Glucose