Near-ultraviolet illumination inhibits the liquid holding recovery in Escherichia coli B.
Liquid holding recovery (LHR) consists of an increase in the survival of UV-irradiated cells when they are held under non-nutrient conditions before plating. In this study we investigated in E. coli B cells the effect of the growth inhibition induced by near UV (365 nm) illumination (growth delay, GD) before irradiation with UV-254 nm on the amplitude of LHR and the induction of an SOS function (filamentation) during the liquid holding period. Our results demonstrated that pre-illumination with near-UV inhibits LHR and the induction of filamentation when the cells are incubated in nutrient medium. Moreover, this inhibition is due to GD, an effect caused by a photoproduct in the E. coli t-RNA, the 8-13 link.
Aragão
B R
BR
Departamento de Biologia Molecular, Universidade Federal da Paraíba, João Pessoa, Brasil.
Caldeira-de-Araújo
A
A
De Mattos
J C
JC
Favre
A
A
Alcantara-Gomes
R
R
D002455:Cell Division / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects*; D003470:Culture Media; D004926:Escherichia coli / Q000166:cytology / Q000528:radiation effects*; D011829:Radiation Dosage; D014466:Ultraviolet Rays
D003470:Culture Media