Effect of phospholipids on UDP-glucose: ceramide glucosyltransferase from Golgi membranes.
1. The removal of phospholipids completely abolished the activity of the enzyme UDP-glucose:ceramide glucosyltransferase from Golgi membranes. 2. Modulation of enzyme activity by phospholipids was undertaken on the solubilized form of the enzyme. 3. Well-defined fatty acyl chains and polar head groups were necessary for maximal stimulation by phospholipids. 4. A specific requirement for phosphatidylcholine is suggested by preliminary experiments of reconstitution of enzyme activity with phosphatidylcholine vesicles.
Durieux
I
I
Laboratoire de Biochimie des Membranes, Université Claude Bernard Lyon I, Villeurbanne, France.
Martel
M B
MB
Got
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R
D000818:Animals; D002784:Cholesterol / Q000494:pharmacology; D002788:Cholesterol Esters / Q000494:pharmacology; D005964:Glucosyltransferases / Q000378:metabolism*; D006056:Golgi Apparatus / Q000201:enzymology*; D007425:Intracellular Membranes / Q000201:enzymology; D007700:Kinetics; D010713:Phosphatidylcholines / Q000494:pharmacology; D010714:Phosphatidylethanolamines / Q000494:pharmacology; D010740:Phospholipases / Q000494:pharmacology; D010743:Phospholipids / Q000494:pharmacology*; D013552:Swine
D002788:Cholesterol Esters; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D002784:Cholesterol; D005964:Glucosyltransferases; C023246:ceramide glucosyltransferase; D010740:Phospholipases