title: Serological diagnosis of dengue by an ELISA inhibition method (EIM).
abstract: An ELISA Inhibition Method (EIM) was proposed for the serologic diagnosis of dengue, comparing its results with the Hemagglutination Inhibition (HI) and the IgM capture-ELISA (MAC-ELISA). Advantages and disadvantages of both methods are discussed according to sensitivity, specificity, performance and usefulness. As a conclusion we recommend the complementary inclusion of the EIM and MAC-ELISA substituting the HI for laboratories engaged in the diagnosis and surveillance of dengue.
authors: Departamento de Virología, Instituto de Medicina Tropical "Pedro Kouri", Siboney, Ciudad de La Habana, Cuba.
authors: R J
authors: RJ
authors: Fernández
authors: S
authors: S
authors: Vázquez
mesh_terms: D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D000914:Antibodies, Viral / Q000032:analysis*; D003715:Dengue / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral; D007075:Immunoglobulin M