title: Purification and characterization of dinitrophenylglutathione ATPase of human erythrocytes and its expression in other tissues.
abstract: S-(2,4-dinitrophenyl)glutathione (Dnp-SG) ATPase of human erythrocytes has been purified to apparent homogeneity by affinity chromatography. In reduced denaturing gels, the subunit Mr value of Dnp-SG ATPase was found to be 38,000. Dinitrophenyl glutathione (Dnp-SG) stimulated the hydrolysis of ATP by the purified enzyme whereas oxidized glutathione (GSSG) did not, indicating that Dnp-SG and GSSG are transported from the erythrocytes by different transporters. Results of Western blot analysis using the antibodies against Dnp-SG ATPase subunits indicated that the enzyme was expressed in human liver, lung, placenta and pancreas.
authors: Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77550.
authors: R
authors: R
authors: Sharma
authors: S
authors: S
authors: Gupta
authors: S V
authors: SV
authors: Singh
authors: R D
authors: RD
authors: Medh
authors: H
authors: H
authors: Ahmad
authors: E F
authors: EF
authors: LaBelle
authors: Y C
authors: YC
authors: Awasthi
mesh_terms: D000251:Adenosine Triphosphatases / Q000302:isolation & purification* / Q000378:metabolism; D001693:Biological Transport, Active; D015153:Blotting, Western; D002846:Chromatography, Affinity; D004910:Erythrocyte Membrane / Q000201:enzymology*; D005978:Glutathione / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D008970:Molecular Weight; D011233:Precipitin Tests; D014018:Tissue Distribution
chemical_list: C030190:S-(2,4-dinitrophenyl)glutathione; D000251:Adenosine Triphosphatases; C068387:S-(dinitrophenyl)glutathione ATPase; D005978:Glutathione