title: Detection of common flagella antigen in Bacillus cereus by monoclonal antibody.
abstract: Bacillus cereus has been classified into 23 types by immunochemical analyses of flagella antigen, but a common antigenic determinant of flagella had not been determined. When the immunochemical method of classification had changed from "agglutination method" to "enzyme-linked immunosorbent assay (ELISA)," the cross-reactivity between each flagella type was increased. These results indicated that the application of ELISA method would enable detection of the common antigenic determinant of B. cereus. Therefore, we attempted to make monoclonal antibody against common flagella antigen that could be detected by ELISA. Monoclonal antibody provided was specific for B. cereus (H-1 to H-23) and did not show the cross-reactivity with Escherichia coli NIH and Bacillus subtilis.
authors: Department of Microbiology, Tohoku College of Pharmacy, Miyagi.
authors: T
authors: T
authors: Mikami
authors: K
authors: K
authors: Hiraoka
authors: T
authors: T
authors: Murakami
authors: J
authors: J
authors: Boon-Long
authors: T
authors: T
authors: Matsumoto
authors: M
authors: M
authors: Suzuki
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D001409:Bacillus cereus / Q000276:immunology*; D001412:Bacillus subtilis / Q000276:immunology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology; D005407:Flagella / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial