Evidence of partial localization in Golgi apparatus of UDP-glucose collagen glucosyltransferase from chick embryo liver.
1. Collagens are the most important components of the connective tissue. 2. Collagen synthesis involves greater than 12 different enzymes whereas three enzymatic systems are involved in the ordered degradation. 3. Some enzymes are found in the rough endoplasmic reticulum (RER). The subcellular localization of disulfur isomerase, alpha D-glucosidase, proteases, galactosyltransferases and glucosyltransferases specific to collagen is unknown. 4. After having determined the best subcellular fractionation conditions for the chick embryo liver, we demonstrate that the galactosylhydroxylysyl glucosyltransferase specific to collagen is located in the RER and in the Golgi apparatus.
Bortolato
M
M
Laboratoire de Biochimie des Membranes (LBTM CNRS UM 380024), Université Claude Bernard Lyon I, Villeurbanne, France.
Azzar
G
G
Farjanel
J
J
Got
R
R
D000818:Animals; D002458:Cell Fractionation; D002642:Chick Embryo; D004721:Endoplasmic Reticulum / Q000201:enzymology; D005964:Glucosyltransferases / Q000032:analysis*; D006056:Golgi Apparatus / Q000201:enzymology*; D008099:Liver / Q000196:embryology / Q000201:enzymology / Q000648:ultrastructure*; D008854:Microscopy, Electron
D005964:Glucosyltransferases; C023247:UDP glucose-collagen glucosyltransferase