title: Antibody isotype responses to the Schistosoma mansoni schistosomulum in the CBA/N mouse induced by different stages of the parasite life cycle.
abstract: During infection with Schistosoma mansoni the extent and nature of immune reactions against schistosomula may be influenced by responses to cross-reactive antigens in eggs or adult worms, and there is now extensive evidence for cross-reactivity between the different stages of the parasite life cycle. In this study IgM and IgG subclass antibodies produced in (CBA/N x Balb/c) F1 male and female mice were measured over a period of time following exposure to a chronic infection, to unisexual male cercariae or to irradiated larvae. Antibody levels were also measured following immunization with antigen preparations derived from adult worms, schistosomula or eggs. (CBA/N x Balb/c) F1 male mice exhibit an X-linked immune deficiency which results in an inability to respond to T-independent (TI) type 2 polysaccharides. Isotype levels were measured by ELISA to detergent-soluble schistosomulum antigen. Results showed that antigens on the different stages of the parasite life cycle have a qualitative influence on the antibody response to the larval surface, and that T-independent type 2 polysaccharides, particularly abundant in egg, exhibit antigen-directed isotype restriction in the form of IgM and IgG3 antibodies.
authors: Molteno Laboratories of Parasitology, Department of Pathology, Cambridge, UK.
authors: G
authors: G
authors: Mazza
authors: D W
authors: DW
authors: Dunne
authors: A E
authors: AE
authors: Butterworth
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D000947:Antigens, Helminth / Q000008:administration & dosage / Q000302:isolation & purification; D005260:Female; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D012550:Schistosoma mansoni / Q000254:growth & development / Q000276:immunology* / Q000528:radiation effects; D012555:Schistosomiasis mansoni / Q000276:immunology* / Q000469:parasitology; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000302:isolation & purification
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014613:Vaccines, Attenuated