The nematode spliced leader RNA participates in trans-splicing as an Sm snRNP.
The trans-spliced leader RNA (SL RNA) of nematodes resembles U snRNAs both in cap structure and in the presence of a consensus Sm binding site. We show here that synthetic SL RNA, synthesized by in vitro transcription, is efficiently used as a spliced leader donor in trans-splicing reactions catalyzed by a cell free extract prepared from developing embryos of the parasitic nematode, Ascaris lumbricoides. Efficient utilization of synthetic SL RNA requires a functional Sm binding site. Mutations within the Sm binding sequence that prevent immunoprecipitation by Sm antisera and prevent cap trimethylation abolish trans-splicing. The effect on trans-splicing is not due to undermethylation of the cap structure.
Maroney
P A
PA
Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, OH 44106.
Hannon
G J
GJ
Denker
J A
JA
Nilsen
T W
TW
D000818:Animals; D001200:Ascaris / Q000235:genetics*; D001324:Autoantigens / Q000502:physiology; D001483:Base Sequence; D001665:Binding Sites; D004252:DNA Mutational Analysis; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009694:Nucleic Acid Precursors / Q000378:metabolism; D009841:Oligonucleotides; D012315:RNA Caps; D012326:RNA Splicing; D012333:RNA, Messenger / Q000378:metabolism; D012261:Ribonucleoproteins / Q000502:physiology*; D017411:Ribonucleoproteins, Small Nuclear; D055517:snRNP Core Proteins
D001324:Autoantigens; D009694:Nucleic Acid Precursors; D009841:Oligonucleotides; D012315:RNA Caps; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; D055517:snRNP Core Proteins