Establishment of an IL-2-dependent, antigen nonspecific chicken T-cell line.
In this article, we describe a-chicken T helper-activated cell line, III-C5, which is non-antigen-specific but IL-2-dependent. By virtue of its absolute requirement for IL-2, the III-C5 cell line is a useful tool for quantifying IL-2 production by any chicken-activated T lymphocytes in culture. The III-C5 line will be used to quantify IL-2 production in mixed lymphocyte reaction in vitro, in order to study the functional activity of T lymphocytes from avian chimeras constructed in our laboratory and particularly for studying their state of tolerance.
Corbel
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Institut d'Embryologie Cellulaire et Moléculaire du CNRS et du Collège de France, Nogent-sur-Marne.
Thomas
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JL
D000818:Animals; D000941:Antigens; D002460:Cell Line; D002645:Chickens / Q000276:immunology*; D002678:Chimera; D007108:Immune Tolerance; D007376:Interleukin-2 / Q000096:biosynthesis / Q000494:pharmacology; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D013601:T-Lymphocytes / Q000276:immunology*
D000941:Antigens; D007376:Interleukin-2