Alterations in the expression of colony-stimulating factor-1 and its receptor during an acute graft-vs-host reaction in mice.
During the course of an acute graft-vs-host reaction in the mouse we observed a progressive increase in the concentration of CSF-1 in serum and liver, peaking at day 14. In contrast, there was a progressive decrease in the splenic CSF-1 concentration. In vivo studies of 125I-CSF-1 uptake and degradation and in vitro studies of 125I-CSF-1 binding by splenic cells demonstrated that within 24 h of the reaction the number of CSF-1 receptor+ cells had increased by 2-fold and their capacity to express the CSF-1 receptor by approximately 3-fold, resulting in a approximately 2.5-fold increase in the splenic clearance of CSF-1 from the circulation. Inasmuch as at 24 h, serum CSF-1 was not significantly altered, these results are suggestive of an increased rate of release of CSF-1 into the circulation early in the response. The splenic CSF-1-receptor bearing cells were in a Mac-1+ fraction that is consistent with a role for CSF-1 in the generation of host-derived splenic macrophages in acute graft-vs-host reaction.
Praloran
V
V
Department of Developmental Biology and Cancer, Albert Einstein College of Medicine, Bronx, NY 10461.
Raventos-Suarez
C
C
Bartocci
A
A
Lucas
J
J
Stanley
E R
ER
Gibbons
J J
JJ
D000818:Animals; D006087:Graft vs Host Reaction; D007457:Iodine Radioisotopes; D016173:Macrophage Colony-Stimulating Factor / Q000032:analysis* / Q000493:pharmacokinetics; D016177:Macrophage-1 Antigen / Q000032:analysis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009929:Organ Size; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000032:analysis*; D013154:Spleen / Q000276:immunology
D007457:Iodine Radioisotopes; D016177:Macrophage-1 Antigen; D016173:Macrophage Colony-Stimulating Factor; D016186:Receptor, Macrophage Colony-Stimulating Factor