title: Interactions between glycolytic enzymes and cytoskeletal structure--the influence of ionic strength and molecular crowding.
abstract: In a study of the interactions between glycolytic enzymes and cytoskeletal structure, the effect of increasing the degree of molecular crowding by the addition of physiological concentrations of saline and protein was studied. Increasing the ionic strength to physiological levels resulted in only a slight decrease in the retention of most of enzymes, whereas the establishment of physiological concentrations of both saline and protein, caused a markedly increased degree of binding of all the glycolytic enzymes. The implications of this data have been discussed in relation to the relative affinities of interaction of the individual components, the influence of molecular crowding and the physiological significance of this phenomenon.
authors: Division of Science and Technology, Griffith University, Brisbane, Australia.
authors: K
authors: K
authors: Shearwin
authors: C
authors: C
authors: Nanhua
authors: C
authors: C
authors: Masters
mesh_terms: D000818:Animals; D002460:Cell Line; D003599:Cytoskeleton / Q000201:enzymology* / Q000648:ultrastructure; D004072:Digitonin / Q000494:pharmacology; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism; D006019:Glycolysis; D007668:Kidney / Q000201:enzymology / Q000648:ultrastructure*; D009994:Osmolar Concentration; D010732:Phosphofructokinase-1 / Q000378:metabolism; D010733:Phosphoglucomutase / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D012965:Sodium Chloride; D013552:Swine; D014305:Triose-Phosphate Isomerase / Q000378:metabolism
chemical_list: D012710:Serum Albumin, Bovine; D012965:Sodium Chloride; D010732:Phosphofructokinase-1; D005634:Fructose-Bisphosphate Aldolase; D010751:Phosphopyruvate Hydratase; D014305:Triose-Phosphate Isomerase; D005956:Glucose-6-Phosphate Isomerase; D010733:Phosphoglucomutase; D004072:Digitonin