Rapid enzymatic analysis of plasma for tyrosine.
In this rapid, simple, and convenient enzymatic method for measurement of tyrosine in plasma, tyrosine is converted to tyramine by action of tyrosine decarboxylase (EC 4.1.1.25) and the tyramine produced is oxidized to p-hydroxybenzyl aldehyde and hydrogen peroxide by action of tyramine oxidase (EC 1.4.3.9). The hydrogen peroxide is reacted with 4-aminoantipyrine and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-m-toluidine in the presence of peroxidase (EC 1.11.1.7) to obtain quinoneimine dye, the absorbance of which is measured at 570 nm. Thus tyrosine is measured in the visible range. The CV was 4.6% or less, and the measurement was unaffected by other amino acids, except for phenylalanine. The values obtained (y) correlated well with those obtained with an amino acid analyzer (x): y = 0.902x + 3.92 mumol/L (Syx = 12.3; r = 0.985; n = 54).
Shimizu
H
H
Diagnostic Research Laboratory, Ono Pharmaceutical Co., Ltd., Osaka, Japan.
Taniguchi
K
K
Sugiyama
M
M
Kanno
T
T
D001322:Autoanalysis; D002021:Buffers; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008995:Monoamine Oxidase; D000587:Oxidoreductases Acting on CH-NH Group Donors; D009195:Peroxidase; D015203:Reproducibility of Results; D013053:Spectrophotometry; D014443:Tyrosine / Q000097:blood*; D014445:Tyrosine Decarboxylase
D002021:Buffers; D014443:Tyrosine; D009195:Peroxidase; D008995:Monoamine Oxidase; D000587:Oxidoreductases Acting on CH-NH Group Donors; D014445:Tyrosine Decarboxylase