Effects of acute and chronic ethanol on cardiac contractile protein ATPase activity of Syrian hamsters.
Ethanol consumption is known to affect cardiac and skeletal muscle. In vivo experiments on cardiac muscle showed that ethanol affects cardiac contractility and Vmax, suggesting that contractile proteins of the myocardium were affected by ethanol. Therefore, experiments were carried out to examine the effects of ethanol on the cardiac contractile protein ATPase activities. Cardiac myofibrils isolated from ethanol-fed hamsters showed a significant decrease in myofibrillar ATPase activities between pCa 6 and 4. On the other hand, addition of ethanol (0.1%) in vitro to cardiac myofibrils from control hamster had no significant effect on the ATPase activities, suggesting that hamsters need to be exposed for longer periods of time to induce demonstratable changes in the contractile protein ATPase activity. Actin-activated myosin ATPase activities were significantly lower in myofibrils from ethanol-fed hamsters at 1:1 and 1:2 ratios of myosin to actin. These investigations revealed that chronic (4 weeks) exposure of hamsters to ethanol reduced cardiac contractile protein ATPase activity, which may help explain impaired cardiac function in chronic alcoholics.
Reddy
Y S
YS
Department of Physiology and Biophysics, University of Oklahoma Health Sciences Center, Oklahoma City 73190.
Beesley
R C
RC
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000437:Alcoholism / Q000201:enzymology* / Q000503:physiopathology; D000818:Animals; D006224:Cricetinae; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D008647:Mesocricetus; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000201:enzymology*; D009210:Myofibrils / Q000187:drug effects / Q000201:enzymology
D000431:Ethanol; D000251:Adenosine Triphosphatases