title: Labeling of hematoporphyrin with 105Rh and binding studies with human gamma globulin.
abstract: Labeling of hematoporphyrin with 105Rh at stoichiometric concentrations is described. Labeling efficiencies of up to 93% could be obtained at pH 9.0 in bicarbonate buffer. Solvent extraction of 105Rh-hematoporphyrin into methyl isobutyl ketone was used to estimate the complex yield. The complex showed high stability and no loss of 105Rh was seen throughout the 6 days of study. 105Rh-hematoporphyrin when incubated with human gamma globulin was seen to be quantitatively bound to the protein. This procedure may be used for labeling monoclonal antibodies with 105Rh for therapeutic applications.
authors: Department of Chemistry, University of Missouri, Columbia 65211.
authors: M R
authors: MR
authors: Pillai
authors: J M
authors: JM
authors: Lo
authors: D E
authors: DE
authors: Troutner
mesh_terms: D006415:Hematoporphyrins / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D007553:Isotope Labeling / Q000379:methods*; D011485:Protein Binding; D011868:Radioisotopes; D012238:Rhodium
chemical_list: D006415:Hematoporphyrins; D007074:Immunoglobulin G; D011868:Radioisotopes; D012238:Rhodium