Biological effects of synthetic AKH in Manduca sexta and estimates of the amount of AKH in corpora cardiaca.
Dose-response curves were measured with synthetic Manduca adipokinetic hormone (AKH) for glycogen phosphorylase activation in larvae and for lipid mobilization in adults. Both responses are known hormonal functions in Manduca sexta. In ligated larvae, full activation of glycogen phosphorylase was achieved with 0.1 pmol and half-maximal activation with 0.03-0.04 pmol. Maximal lipid mobilization in adults required 10 pmol and half-maximal mobilization 0.15 to 0.2 pmol, respectively. An estimate of AKH content of corpora cardiaca from M. sexta was gained by comparing the dose-response curves for synthetic Manduca AKH with curves from gland extracts. Corpora cardiaca extracts were also quantitated by high performance liquid chromatography. According to both estimates corpora cardiaca of adults contain 10-20 pmol AKH per pair, while a pair of larval corpora cardiaca contains 0.7-2 pmol.
Ziegler
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Department of Biochemistry, University of Arizona, Tucson 85721.
D000273:Adipose Tissue / Q000187:drug effects / Q000201:enzymology; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D007301:Insect Hormones / Q000032:analysis* / Q000494:pharmacology*; D007814:Larva; D009036:Moths / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D009842:Oligopeptides / Q000032:analysis* / Q000494:pharmacology*; D006005:Phosphorylases / Q000378:metabolism*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives
D007301:Insect Hormones; D009842:Oligopeptides; C044833:adipokinetic hormone; D006005:Phosphorylases; D011761:Pyrrolidonecarboxylic Acid