title: Disturbed intracellular calcium-related processes of hepatocytes and neutrophils in human alcoholic liver disease.
abstract: Isolated human hepatocytes and separated neutrophils of 11 patients with alcoholic liver disease (ALD) were used to study some aspects of cellular calcium-related processes compared to nonalcoholic controls. 45Ca2+ efflux from the cells decreased in ALD and the calmodulin-inhibitor trifluoperazine did not influence it further. The intracellular free calcium concentration [( Ca2+]i) of nonstimulated hepatocytes and neutrophils proved to be higher in ALD with the Quin2/AM loading technique. However, the [Ca2+]i rise in hepatocytes and neutrophils, with stimulation by low density lipoprotein (LDL) and N-formyl-methionyl-leucyl phenylalanine (FMLP), respectively, was diminished in ALD compared to appropriate controls. The slower 45Ca2+ extrusion rate, higher basal [Ca2+]i levels, and the diminished [Ca2+]i elevation of activated hepatocytes and neutrophils, suggest disturbed calcium-related intracellular processes in ALD, in particular, impaired regulation of the plasma membrane Ca2(+)-ATPase.
authors: First Department of Medicine, University Medical School, Debrecen, Hungary.
authors: G
authors: G
authors: Baffy
authors: Z
authors: Z
authors: Varga
authors: G
authors: G
authors: Fóris
authors: A
authors: A
authors: Leövey
mesh_terms: D000328:Adult; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000032:analysis; D005260:Female; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology; D008099:Liver / Q000378:metabolism*; D008108:Liver Diseases, Alcoholic / Q000097:blood / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000378:metabolism*
chemical_list: D008077:Lipoproteins, LDL; D009240:N-Formylmethionine Leucyl-Phenylalanine; D000252:Calcium-Transporting ATPases; D002118:Calcium