Lysis protein S of phage lambda functions in Saccharomyces cerevisiae.
The lambda S lysis gene was cloned into a Saccharomyces cerevisiae expression vector under GAL1 control. Induction with galactose in S. cerevisiae terminated cell growth and prevented colony formation. Several membrane proteins immunoreactive with anti-S antibody accumulated in the membranes, indicating that sodium dodecyl sulfate-resistant oligomers of S are formed, similar to those observed in the membranes of Escherichia coli cells killed by expression of the S gene. These observations suggest that the S gene product functions as a cytotoxic protein in the yeast cytoplasmic membrane as it does in the bacterial membrane.
Garrett
J
J
Department of Biology, Hamilton College, Clinton, New York 13323.
Bruno
C
C
Young
R
R
D000595:Amino Acid Sequence; D001433:Bacteriolysis; D010582:Bacteriophage lambda / Q000502:physiology*; D001483:Base Sequence; D003001:Cloning, Molecular; D003603:Cytotoxins / Q000378:metabolism*; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013045:Species Specificity; D014764:Viral Proteins / Q000378:metabolism*
D003603:Cytotoxins; D014764:Viral Proteins