title: Mitogenic induction of ornithine decarboxylase in human mononuclear leukocytes: relationships with adenosine diphosphate ribosyltransferase.
abstract: 1. Relationships between ornithine decarboxylase (ODC) and adenosine diphosphate ribosyl transferase (ADPRT) in human mononuclear leukocytes (HML) were tested by statistical comparisons of their values in a group of 46 people, and by use of inhibitors of ADPRT. 2. ODC was assayed following exposure of HML, for 20 hr, to mitogens [phytohemagglutinin (PHA) and pokeweed mitogen]; ADPRT was measured following exposure of HML to H2O2 (100 microM) for 1 hr (activated ADPRT), and in parallel cultures without H2O2 (constitutive ADPRT). 3. Significant correlations were found between ODC and ADPRT values; the effects of smoking disturbed the correlations. PHA induction of ODC was negatively influenced by age (standardized beta coefficient = -2.95, P = 0.005), while age also influenced ADPRT values negatively in non-smokers (for H2O2 activated ADPRT, standardized beta coefficient = -2.74, P less than 0.008). 4. Inhibitors of ADPRT, nicotinamide, caffeine and benzamide inhibited the induction of ODC by PHA in a concentration-dependent manner, in the range (0.6-10 mM) known to inhibit ADPRT.
authors: Preventive Medicine Institute/Strange Clinic, New York, NY 10016.
authors: D B
authors: DB
authors: Johnson
authors: M M
authors: MM
authors: Markowitz
authors: P E
authors: PE
authors: Joseph
authors: D G
authors: DG
authors: Miller
authors: R W
authors: RW
authors: Pero
mesh_terms: D000367:Age Factors; D001549:Benzamides / Q000494:pharmacology; D015415:Biomarkers; D002110:Caffeine / Q000494:pharmacology; D002478:Cells, Cultured; D004790:Enzyme Induction; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000201:enzymology*; D015999:Multivariate Analysis; D009536:Niacinamide / Q000494:pharmacology; D009955:Ornithine Decarboxylase / Q000096:biosynthesis* / Q000097:blood; D010835:Phytohemagglutinins / Q000494:pharmacology*; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*
chemical_list: D001549:Benzamides; D015415:Biomarkers; D010835:Phytohemagglutinins; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; C047876:phytohemagglutinin-M; D009536:Niacinamide; D002110:Caffeine; D011065:Poly(ADP-ribose) Polymerases; D009955:Ornithine Decarboxylase