Development of a radioimmunoassay for neurone specific enolase (NSE) and its application in the study of patients receiving intra hepatic arterial streptozotocin and floxuridine.
A radioimmunoassay has been developed for neurone specific enolase (NSE) and used to measure serum NSE levels in patients with neuroendocrine and non-neuroendocrine tumours following intra hepatic arterial chemotherapy. Ten patients were studied, 7 receiving streptozotocin and floxuridine for neuroendocrine tumours and three receiving cisplatinum for non-neuroendocrine neoplasms. All ten patients had liver metastases. In patients with tumours of neuroendocrine origin, a significant increase in serum NSE was recorded within 24 h of therapy. Slight increases in serum NSE levels were also recorded in three patients with non neuroendocrine tumours. These increases may reflect lysis of neuroendocrine cells within the tumour. Raised levels in non-neuroendocrine tumour patients may reveal damage done to healthy neuronal and neuroendocrine cells during treatment. NSE may be a useful marker of the extent of cell death following chemotherapy.
Cunningham
R T
RT
Department of Medicine, Queen's University of Belfast, Ireland.
Johnston
C F
CF
Irvine
G B
GB
McIlrath
E M
EM
McNeill
A
A
Buchanan
K D
KD
D000818:Animals; D001923:Brain Chemistry; D002945:Cisplatin / Q000008:administration & dosage / Q000627:therapeutic use; D004700:Endocrine System Diseases / Q000097:blood / Q000188:drug therapy / Q000201:enzymology; D005260:Female; D005467:Floxuridine / Q000008:administration & dosage / Q000627:therapeutic use*; D006499:Hepatic Artery; D006801:Humans; D007269:Injections, Intra-Arterial; D008297:Male; D009369:Neoplasms / Q000097:blood / Q000188:drug therapy / Q000201:enzymology; D009490:Neurosecretory Systems / Q000473:pathology; D010751:Phosphopyruvate Hydratase / Q000097:blood* / Q000302:isolation & purification; D011817:Rabbits; D011863:Radioimmunoassay; D012015:Reference Standards; D013311:Streptozocin / Q000008:administration & dosage / Q000627:therapeutic use*
D005467:Floxuridine; D013311:Streptozocin; D010751:Phosphopyruvate Hydratase; D002945:Cisplatin