title: [Evaluation in our milieu of an ELISA method (ANDA-Tb) for the serological diagnosis of tuberculosis].
abstract: We have evaluated the new ELISA test (ANDA-Tb) for the serological diagnosis of tuberculosis in serum samples from 343 individuals: 160 were from blood donors, 152 from patients with nontuberculous diseases, most of them respiratory, and 31 from tuberculous patients diagnosed by positive culture. Using a cutoff value in 350 serounits we had a positive predictive value of 57% and a negative predictive value of 98% in blood donors, and 43% and 97%, respectively, in control patients. Sensitivity was 68%. There was a higher false positive rate in patients with respiratory diseases, particularly acute pneumonia. We conclude that this particular ELISA is very useful as a supportive test for the diagnosis of tuberculosis, with a lower specificity in hospital nontuberculous patients.
authors: Laboratorio de Microbiología, Hospital Ciudad de Coria.
authors: J A
authors: JA
authors: Pérez
authors: J J
authors: JJ
authors: Moreno
authors: A
authors: A
authors: Aguyo
authors: M
authors: M
authors: Alvarez
authors: P
authors: P
authors: Díez
authors: C
authors: C
authors: Cuenca
authors: I
authors: I
authors: Sanchidrián
authors: L
authors: L
authors: Rodríguez
mesh_terms: D000907:Antibodies, Bacterial / Q000032:analysis*; D001782:Blood Donors; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000276:immunology* / Q000302:isolation & purification; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D012120:Respiration Disorders / Q000276:immunology; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D011933:Reagent Kits, Diagnostic
vernacular_title: Evaluación en nuestro medio de un método ELISA (ANDA-Tb) para diagnóstico serológico de tuberculosis.