Primary mixed lymphocyte responses to HLA-DP.
Combinations of peripheral blood lymphocytes, matched or mismatched for HLA-DP, were analyzed in primary one-way mixed lymphocyte culture experiments. Proliferative responses as correlated with tritiated thymidine uptake were assessed over a kinetic range of 5-15 days. A proliferative response was observed between DP-mismatched combinations, whereas combinations matched for DP and all other HLA alloantigens did not elicit significant proliferation. Optimal responses were observed 9 days after the combination of 1 x 10(5) responder and stimulator cells. Responses were blocked by anti-DP monoclonal antibodies. These studies demonstrate the complexity of the primary mixed lymphocyte culture system and suggest that DP alloantigens should be considered when anomalous responses are obtained.
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Immunogenetics Research Section, Blood Center of Southeastern Wisconsin, Milwaukee 53233.
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D000911:Antibodies, Monoclonal; D006681:HLA-D Antigens / Q000276:immunology; D006682:HLA-DP Antigens / Q000276:immunology*; D006650:Histocompatibility Testing; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed / Q000379:methods*; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
D000911:Antibodies, Monoclonal; D006681:HLA-D Antigens; D006682:HLA-DP Antigens