[Host factors that changes the distribution of immunotoxin].
Mouse monoclonal antibody A 7, which was raised against human colon cancer, was used for preparing the conjugates with neocarzinostatin, mitomycin C and adriamycin. The tissue distribution of these three conjugates were examined in athymic nude mice transplanted with human colon cancer. The distribution in tumor was different in these three conjugates. The route of administration of the conjugate affected its distribution in tumor tissues. In the case of tumor transplanted in the back of mice, intravenous administration seemed to superior to intraperitoneal one. In clinical trials of immunoconjugate composed of A 7 and polypeptide anticancer drug neocarzinostatin (A 7-NCS), human anti-mouse antibody (HAMA) was observed in most cases without serious immune response such as anaphylactic shock. Human antibody against neocarzinostatin could not be detected in any case receiving A 7-NCS.
Yamaguchi
T
T
First Dept. of Surgery, Kyoto Prefectural University of Medicine.
Takahashi
T
T
Yokota
T
T
Kitamura
K
K
Noguchi
A
A
Doi
M
M
Chimori
Y
Y
Honda
M
M
Noguchi
A
A
Otsuji
E
E
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology / Q000493:pharmacokinetics; D003110:Colonic Neoplasms / Q000276:immunology / Q000378:metabolism*; D004317:Doxorubicin / Q000008:administration & dosage / Q000493:pharmacokinetics; D006801:Humans; D000922:Immunotoxins / Q000008:administration & dosage / Q000276:immunology / Q000493:pharmacokinetics*; D051379:Mice; D008819:Mice, Nude; D016685:Mitomycin; D008937:Mitomycins / Q000008:administration & dosage / Q000493:pharmacokinetics; D009368:Neoplasm Transplantation; D014018:Tissue Distribution; D009353:Zinostatin / Q000008:administration & dosage / Q000276:immunology / Q000493:pharmacokinetics
D000911:Antibodies, Monoclonal; D000922:Immunotoxins; D008937:Mitomycins; D016685:Mitomycin; D004317:Doxorubicin; D009353:Zinostatin