[Antiproliferative activity of a highly purified coal tar preparation in comparison with clobetasol-17-propionate].
Coal tar and glucocorticosteroids are among the preparations used for the treatment of hyperproliferative inflammatory dermatosis. Coal tar generally seems to have a better effect on psoriasis capillitii than betamethasone-17-valerate. This finding is confirmed by investigations with human skin fibroblasts. The coal tar preparation Berniter has a strongly inhibitory effect on cell proliferation. The action of the preparation with tar can be clearly distinguished from the effect of the vehicle. At the ED50 concentration of the substance with tar, the vehicle without tar causes no inhibition. Based on weight equivalents Berniter has a greater inhibitory effect on proliferation of human skin fibroblasts than clobetasol-17-propionate. In the case of both substances protein biosynthesis is only affected at concentrations higher than those needed for the inhibition of proliferation. Berniter and the vehicle without tar have an equally strong effect on protein synthesis. Berniter affects the nucleoside triphosphatase bound to the nuclear membrane, and thus influences the nucleocytoplasmic transport of mRNA. The effect of the vehicle and the preparation containing tar on this parameter are very similar. Berniter has a positive effect on cell mobility. In preconfluent cultures the mobility of the cell is slightly increased and in confluent cultures significantly. Our results show that Berniter inhibits proliferation in cell cultures of human skin fibroblasts to a higher degree than clobetasol-17-propionate. It is to be assumed that neither the inhibition of protein synthesis nor the inhibition of mRNA transport play a causal role in the antiproliferative effect of coal tar. Inhibition of proliferation as a result of general damage to the cell can also be practically ruled out.
Bernd
A
A
Zentrum der Dermatologie und Venerologie, Johann Wolfgang Goethe-Universität Frankfurt/Main.
Theilig
C
C
Müller
K
K
Bereiter-Hahn
J
J
Hevert
F
F
Holzmann
H
H
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects; D002990:Clobetasol / Q000031:analogs & derivatives* / Q000494:pharmacology; D003033:Coal Tar / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
D000596:Amino Acids; D012333:RNA, Messenger; D003033:Coal Tar; D002990:Clobetasol; D000251:Adenosine Triphosphatases; D013936:Thymidine
Antiproliferative Aktivität einer hochgereinigten Steinkohlenteer-Präparation im Vergleich mit Clobetasol-17-Propionat.