[Effect of sodium diphenylhydantoin on sister chromatid exchange frequency in mice].
In order to analyze the effect of the anticonvulsant diphenyl hydantoin (DPH) over the frequency of sister chromatid exchanges (SCE) in vivo, Balb-C mouse 8-12 weeks old, males and females, were injected intraperitoneally with a single dose and three different concentrations: 1, 10 and 20 mg/Kg, as negative and positive control, we used physiological serum and mitomycin-C respectively that were injected in the same way. Twenty five metaphases were analyzed per individual in the slide with the differential staining of sister chromatid, we found a light, but significantly increase, in the frequency of SCE in the concentrations of 10 and 20 mg/Kg when they were compared with the ones obtained for the negative control. In the males was observed a positive correlation in the levels of ICH when the doses of DPH were increased.
Leal-Garza
C
C
Unidad de Investigación Biomédica del Noreste, IMSS, Monterrey, México.
Valenciano-Cedillo
G
G
Cerda-Flores
R
R
Garza-Chapa
R
R
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016685:Mitomycin / Q000494:pharmacology; D010672:Phenytoin / Q000494:pharmacology*; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D013268:Stimulation, Chemical
D016685:Mitomycin; D010672:Phenytoin
Efecto del difenilhidantoinato de sodio (DFH) sobre la frecuencia de intercambio de cromátides hermanas (ICH) en ratones.