Effect of salmon calcitonin on the infiltration of T lymphocytes in a synovium-like membrane of a rat inflammatory model.
The effect of salmon calcitonin (SCT) on the number of T lymphocytes accumulating in air pouch lining membranes produced on the back of Wistar rats was studied. SCT was given from day 0 through day seven following the air cavitation by daily injections of 2.5-20 micrograms/kg directly into the air cavity. Compared with saline controls, treatment with SCT caused a clear reduction in percentage of T lymphocytes, T helper cells and T non-helper subset in the synovial-like air pouch lining as revealed by FITC-labeled monoclonal antibodies and quantified by flow cytophotometric measurements. This effect may be due to a reduction in local vascular permeability caused by SCT.
Grieder
A
A
Preclinical Research Division, Sandoz Ltd., Basel, Switzerland.
Felder
M
M
Schaffner
W
W
D000388:Air; D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000276:immunology; D002116:Calcitonin / Q000494:pharmacology*; D005260:Female; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007249:Inflammation / Q000209:etiology / Q000473:pathology*; D007958:Leukocyte Count; D051381:Rats; D011919:Rats, Inbred Strains; D013583:Synovial Membrane / Q000473:pathology*; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology*; D006377:T-Lymphocytes, Helper-Inducer / Q000473:pathology; D013861:Thiocyanates
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D005452:Fluoresceins; D005456:Fluorescent Dyes; D013861:Thiocyanates; C028815:salmon calcitonin; D002116:Calcitonin; D016650:Fluorescein-5-isothiocyanate