Rapid isolation and chemical synthesis of two porcine cardiodilatins, the cardiac hormone precursor and related fragment.
To evaluate the chemically synthesized materials, two cardiodilatins, CDD-126 and CDD-88/CDD(39-126), a precursor of atrial hormone and a related fragment, were isolated from porcine atria by immunoaffinity chromatography or alginic acid adsorption followed by an ion exchange high performance liquid chromatography. The chemical synthesis was carried out using an automated peptide synthesizer. After cleavage and refolding, the crude CDDs were directly characterized by the novel method of primary structure determination using electroblotting and microsequencing. The purified synthetic CDDs were identical with the natural ones in physicochemical and immunochemical properties, as well as their biological actions both in vivo and in vitro.
Nokihara
K
K
GBF-Gesellschaft für Biotechnologische Forschung, Braunschweig, F.R. Germany.
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D009320:Atrial Natriuretic Factor / Q000138:chemical synthesis / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006325:Heart Atria / Q000032:analysis; D007118:Immunoassay; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000138:chemical synthesis / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000302:isolation & purification*; D011498:Protein Precursors / Q000138:chemical synthesis / Q000302:isolation & purification; D013552:Swine
D000911:Antibodies, Monoclonal; D009124:Muscle Proteins; D010446:Peptide Fragments; D011498:Protein Precursors; D009320:Atrial Natriuretic Factor; C040651:cardiodilatin; C054396:atrial natriuretic factor 88