Human neutrophil hydrogen peroxide generation following physical exercise.
The effects of prolonged, submaximal exercise by seven healthy, untrained individuals on the generation of H2O2 by neutrophils was studied. Hydrogen peroxide generation by neutrophils isolated from pre-exercise (control) and post-exercise blood samples was measured 10, 15 and 20 minutes following stimulation with phorbol myristate acetate (PMA). Exercise was associated with a significant elevation in the number of circulating neutrophils and a diminished capacity for neutrophil H2O2 generation following PMA stimulation. Addition of post-exercise plasma to neutrophils isolated from pre-exercise blood caused a small reduction in H2O2 generation, suggesting the presence of an inhibitory factor(s) in the plasma during physical exercise. These results support the concept that exercise may contribute to an attenuation of oxygen-dependent neutrophil killing.
Macha
M
M
Department of Physiology, University of Michigan Medical School, Ann Arbor.
Shlafer
M
M
Kluger
M J
MJ
D000210:Acute-Phase Reaction / Q000276:immunology; D000328:Adult; D015444:Exercise / Q000502:physiology*; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007958:Leukocyte Count; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D010703:Phorbol Esters / Q000494:pharmacology
D010703:Phorbol Esters; D006861:Hydrogen Peroxide