Gel and buffer effects on the migration of DNA molecules in agarose.
The migration rate of linear and supercoiled DNA molecules in four brands of agarose from two different lots was compared using three buffer systems. Differences in DNA mobility were observed between brands and lots of agarose, in standard as well as in pulsed-field gel electrophoresis. A buffer-related effect on the migration of DNA molecules in agarose gels was also observed. Our results underline the need for an actual lot analysis of agarose, this extraction product varying from one preparation to another in several aspects.
Hamelin
C
C
Centre de recherche en virologie, Institut Armand-Frappier, Laval-des-Rapides, Québec, Canada.
Yelle
J
J
D010582:Bacteriophage lambda / Q000235:genetics; D002021:Buffers; D004278:DNA, Superhelical / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry; D004058:Diffusion; D004587:Electrophoresis, Agar Gel
D002021:Buffers; D004278:DNA, Superhelical; D004279:DNA, Viral