Improved prolactin crop-sac bioassay applying a morphometric approach.
A pigeon crop-sac bioassay for prolactin was developed applying a morphometric approach for quantification of the epithelial cell proliferation induced by the hormone. A small male dove, indigenous from South America (Zenaida auriculata) previously castrated was used as experimental animal. The total dose of prolactin was divided into 4 daily injections administered systemically into the pectoral muscles. The number of the proliferated cell layers used as the bioassay end point was analyzed statistically. This procedure yields a highly sensitive bioassay with an excellent precision index, particularly well suited for quantification of the different molecular forms of pituitary PRL.
Prada
M I
MI
Centro de Microscopía Electrónica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.
Torres
A I
AI
Aoki
A
A
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002455:Cell Division / Q000187:drug effects; D010856:Columbidae; D003426:Crop, Avian / Q000187:drug effects* / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D004848:Epithelium / Q000187:drug effects / Q000648:ultrastructure; D007273:Injections, Intramuscular; D008297:Male; D011388:Prolactin / Q000008:administration & dosage / Q000032:analysis* / Q000494:pharmacology; D013268:Stimulation, Chemical
D011388:Prolactin