[Dot-ELISA using monoclonal antibody for detecting schistosome circulating antigen].
Monoclonal antibodies were produced by the fusion of splenic lymphocytes from BALB/c mice immunized with Schistosoma japonicum excretory/secretory antigen and the myeloma cell line SP2/0. The 1B2E7B8 McAb was proved to be specific against the gut antigen of adult worm in IFA. The McAb labelled with HRP was used in Dot-ELISA to detect schistosome circulating antigen. Schistosome circulating antigen was detected in 152 out of 188 proven cases of schistosomiasis, accounting for a positive rate of 80.9%. The positive rates for circulating antigen in cases with 1-24, 25-99 and greater than or equal to 100 EPG were 76.8%, 86.6% and 100% respectively. 10 out of 11 cases who had been checked 2 months after effective treatment became ELISA negative. No circulating antigen was detected in cases with other parasitosis nor in normal individuals. In addition, the McAb-Dot-ELISA showed good reproducibility. The results indicated that McAb-Dot-ELISA might be used for diagnosis of schistosomiasis and evaluation of cure.
Zhou
R
R
Institute of Antiparasitic Diseases, Sichuan Academy of Medical Sciences.
Miao
D Q
DQ
Zhang
S H
SH
Zhang
Y W
YW
Zhu
R S
RS
D000818:Animals; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000175:diagnosis*
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth