The influence of growth conditions of Pasteurella multocida on its ability to colonise the nasal mucosa of SPF piglets.
Colonisation of type D Pasteurella multocida was studied in five groups of seven SPF piglets each. Piglets of Group 1 were kept together with seven 5-week-old piglets obtained from a large herd infected with toxigenic P. multocida for 16 weeks (contact infection). These piglets were made free from toxigenic Bordetella bronchiseptica by local immunisation. Piglets of Group 2 were inoculated with 5 x 10(7) colony-forming units (cfu) of P. multocida washed from the nasal mucosa of piglets free from toxigenic B. bronchiseptica with fetal calf serum. Piglets of Group 3 were inoculated intranasally with 5 x 10(7) cfu of P. multocida washed from yeast-extract proteose-peptone cystine (YPC)-blood agar with fetal calf serum. Piglets of Group 4 were inoculated with 5 x 10(7) cfu of P. multocida grown in a YPC-based broth without blood. Piglets of Group 5 served as controls. The piglets of Group 1 did not contract P. multocida infection from infected contact piglets. After a single inoculation one of four, while after three inoculations two of three piglets of Group 2 became infected by P. multocida. After a single inoculation none of four, while after three inoculations one of three piglets of Group 3 were colonised by P. multocida. Both single and repeated inoculation failed in piglets of Group 4.
Eliás
B
B
Department of Research Organization, University of Veterinary Science, Budapest, Hungary.
Herpay
M
M
Albert
M
M
Fekete
J
J
Tuboly
S
S
Rafai
P
P
Molnár
E
E
D000818:Animals; D015169:Colony Count, Microbial; D009297:Nasal Mucosa / Q000382:microbiology*; D010325:Pasteurella / Q000254:growth & development*; D010326:Pasteurella Infections / Q000382:microbiology / Q000662:veterinary*; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*