Amplification and analysis of human DNA present in mosquito bloodmeals.
DNA fingerprinting should permit the identification of individual human hosts of haematophagous arthropods, providing epidemiologically useful information, for example, the biting rates on different people and the impact of insecticide-impregnated bednets. Investigations reported here demonstrate that it is possible to extract, amplify and fingerprint human DNA from the bloodmeals of individual female Anopheles gambiae mosquitoes kept at 24 degrees C for up to 10-15 h post-ingestion.
Coulson
R M
RM
Department of Biochemistry, Imperial College of Science, Technology and Medicine, London.
Curtis
C F
CF
Ready
P D
PD
Hill
N
N
Smith
D F
DF
D000818:Animals; D000852:Anopheles / Q000502:physiology*; D004247:DNA / Q000097:blood*; D016172:DNA Fingerprinting; D005247:Feeding Behavior; D005260:Female; D006801:Humans; D007299:Insect Bites and Stings / Q000453:epidemiology*; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results
D004247:DNA