Production of specific anti-rat 5-HT1A receptor antibodies in rabbits injected with a synthetic peptide.
Polyclonal antibodies were raised by the repeated injection of rabbits with a synthetic peptide corresponding to a highly selective portion (amino acid residues 243 to 268) of the amino acid sequence of the rat 5-HT1A receptor. The anti-peptide antiserum allowed the immunoprecipitation of 5-HT1A receptors but not of other 5-HT1 sites solubilized from rat hippocampal membranes. Immunoautoradiographic labelling of rat brain sections with the anti-peptide antiserum was superimposed with the autoradiographic distribution of 5-HT1A sites labelled by the selective radioligand [3H]8-OH-DPAT.
el Mestikawy
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S
INSERM U288, Neurobiologie Cellulaire et Fonctionnelle, Faculté de Médecine Pitié-Salpêtrière, Paris, France.
Riad
M
M
Laporte
A M
AM
Vergé
D
D
Daval
G
G
Gozlan
H
H
Hamon
M
M
D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D001345:Autoradiography; D007267:Injections; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology*; D011233:Precipitin Tests; D011817:Rabbits / Q000276:immunology; D051381:Rats / Q000276:immunology; D011919:Rats, Inbred Strains; D011985:Receptors, Serotonin / Q000276:immunology*; D013764:Tetrahydronaphthalenes / Q000378:metabolism; D014316:Tritium
D010446:Peptide Fragments; D011985:Receptors, Serotonin; D013764:Tetrahydronaphthalenes; D014316:Tritium; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin