Significance and specificity of anti-SSA/Ro antibody in patients with bullous disorders.
Sera from 45 patients with pemphigus and from 25 patients with bullous pemphigoid were screened for the presence of anti-SSA/Ro autoantibody by double immunodiffusion and SSA/Ro enzyme-linked immunosorbent assay and immunoblot analysis. Totally 6 of them were found to have IgG4 subclass anti-SSA/Ro antibody by SSA/Ro enzyme-linked immunosorbent assay, and this was further confirmed by immunoblot analysis. None of these 6 patients have any evidence of symptoms or signs for connective tissue disease.
Deng
J S
JS
Department of Dermatology, School of Medicine, University of Pittsburgh, Pa.
Lee
P Y
PY
Fratto
J L
JL
D000328:Adult; D000368:Aged; D000974:Antibodies, Antinuclear / Q000032:analysis* / Q000276:immunology; D000918:Antibody Specificity; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000032:analysis; D008875:Middle Aged; D010391:Pemphigoid, Bullous / Q000276:immunology; D010392:Pemphigus / Q000276:immunology; D012872:Skin Diseases, Vesiculobullous / Q000276:immunology*
D000974:Antibodies, Antinuclear; D007074:Immunoglobulin G; C035356:SS-A antibodies