Regulation of interleukin 2-driven T-lymphocyte proliferation by prolactin.
The requirement for prolactin in interleukin 2-driven T-cell proliferation was evaluated. Addition of an anti-prolactin antiserum resulted in the specific inhibition of T-cell proliferation in a time- and dose-dependent manner. Synthesis of prolactin and its mRNA, however, did not occur during interleukin 2 stimulation. Instead, previously internalized prolactin, presumably from fetal bovine serum, appears to serve as the source of prolactin under serum-free conditions. A 7-fold increase in a prolactin receptor occurred as a function of cell cycle progression; accumulation of a 1.6-kilobase prolactin receptor mRNA increased approximately 2-fold. Interleukin 2 stimulation induced the translocation of prolactin into the nucleus and prolactin receptor to the nuclear periphery. These data indicate that extracellular prolactin is requisite for T-cell proliferation and suggest that the effects of prolactin are exerted in the nucleus.
Clevenger
C V
CV
Department of Pathology, University of Pennsylvania, Philadelphia 19104.
Russell
D H
DH
Appasamy
P M
PM
Prystowsky
M B
MB
D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D003208:Concanavalin A; D005434:Flow Cytometry; D015870:Gene Expression; D007106:Immune Sera; D007376:Interleukin-2 / Q000494:pharmacology*; D008213:Lymphocyte Activation; D051379:Mice; D011388:Prolactin / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D011981:Receptors, Prolactin / Q000235:genetics / Q000502:physiology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
D007106:Immune Sera; D007376:Interleukin-2; D012333:RNA, Messenger; D011981:Receptors, Prolactin; D003208:Concanavalin A; D011388:Prolactin