title: A new trisaccharide sugar chain linked to a serine residue in the first EGF-like domain of clotting factors VII and IX and protein Z.
abstract: Recently, we determined the complete amino acid sequence of bovine factor VII (Takeya, H. et al. (1988) J. Biol. Chem. 263, 14868-14877). In the course of the studies, we found an unknown serine derivative at position 52 in the first epidermal growth factor-like domain of factor VII. A pentapeptide isolated from the S-aminoethylated factor VII contained Ser-52, which could not be identified with a gas-phase sequencer. The same results were also obtained for a pentapeptide containing Ser-53 of factor IX and protein Z. Component sugar analysis revealed that the peptide contained 1 mol of glucose and 2 mol of xylose. This sugar component was also confirmed by high-resolution fast atom bombardment mass spectrometric analysis of the pentapeptide. The trisaccharide was released from the peptides by means of beta-elimination reaction and its reducing end was identified as pyridylamino-glucose. These results indicate the existence of a (Xyl2)Glc-Ser structure in factors VII, IX and protein Z. Similar results were obtained for human factors VII, IX and protein Z. This is the first report of a (Xyl2)-Glc-Ser structure in glycoproteins to our knowledge. The presence of the unique trisaccharide structure in factors VII, IX and protein Z leads us to anticipate its biological role in the tissue factor pathway.
authors: Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan.
authors: S
authors: S
authors: Iwanaga
authors: H
authors: H
authors: Nishimura
authors: S
authors: S
authors: Kawabata
authors: W
authors: W
authors: Kisiel
authors: S
authors: S
authors: Hase
authors: T
authors: T
authors: Ikenaka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004815:Epidermal Growth Factor / Q000737:chemistry*; D005164:Factor IX / Q000737:chemistry*; D005167:Factor VII / Q000737:chemistry*; D006801:Humans; D008961:Models, Structural; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000302:isolation & purification; D011487:Protein Conformation; D012689:Sequence Homology, Nucleic Acid; D012694:Serine; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D001798:Blood Proteins; D009844:Oligosaccharides; D010446:Peptide Fragments; C026080:plasma protein Z; D012694:Serine; D004815:Epidermal Growth Factor; D005167:Factor VII; D005164:Factor IX