[Non-specific immunosuppressive effect induced by A. actinomycetemcomitans].
Previous studies have shown that immunosuppressive effects are related to the pathogenicity of periodontopathic bacteria and the development of periodontal disease. We reported soluble sonic extracts (SE) from A. actinomycetemcomitans and B. intermedius had a strong immunosuppressive effect on primary response of anti-SRBC plaque forming cells. SE from A. actinomycetemcomitans inhibited the IgM----IgG isotype switching. The purpose of this study was to investigate the effect of SE on secondary immunoresponse. As a control, mice (C3H/HeN) were immunized with intraperitoneal injection of SRBC and complete Freund's adjuvant, and additional SRBC after 30 days. In order to study the effect of SE from A. actinomycetemcomitans on secondary response, four groups were prepared in this study. One group of C3H/HeN mice were injected SE intravenously before the primary immunization of SRBC. The other three groups were given SE at different time on secondary injection of SRBC and designated the pre-, post-, or simultaneous injection, respectively. Hemolytic plaque assay was performed and estimated anti-SRBC plaque forming cells in each immunoglobulin class and IgG subclass, on the 5th day after the secondary injection. The immunosuppressive effect was found in all the groups which we examined. The present study strongly suggests that periodontopathic bacteria have strong adjuvant activity, and long-term Actinobacillus infection accompanied by periodontal fluctuation in bacterial flora may induce aberration in the immune system. The suppressed immune system explain the explosive growth of bacteria tested in our investigation, resulting in the mixed or opportunistic infection by bacteria harbored in the gingival crevice.
Ochiai
K
K
Department of Microbiology, Nihon University School of Dentistry at Matsudo, Japan.
Kurita
T
T
Kamino
Y
Y
Ikeda
T
T
D000189:Actinobacillus Infections / Q000276:immunology*; D016976:Aggregatibacter actinomycetemcomitans / Q000276:immunology* / Q000472:pathogenicity*; D000818:Animals; D003773:Dental Plaque / Q000276:immunology / Q000382:microbiology; D007108:Immune Tolerance; D051379:Mice; D010510:Periodontal Diseases / Q000276:immunology* / Q000382:microbiology