[Determining free activity of cathepsin D in tissue homogenates after precipitation of cellular organelles in pH 5,0].
Homogenate is prepared in 0.25 M sucrose. Cellular organelles are precipitated by acidification to pH 5.0 and removed by centrifugation at 1500 x g during 30 minutes. In the obtained cytosol which does not contain lysosomes free activity of cathepsin D is determined.
Skrzydlewska
E
E
Zakładu Analizy Instrumentalnej Akademii Medycznej, Białymstoku.
Worowski
K
K
Jurkowski
J
J
Ostrowska
H
H
D000818:Animals; D002402:Cathepsin D / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D003470:Culture Media; D003600:Cytosol / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology* / Q000648:ultrastructure; D008954:Models, Biological
D003470:Culture Media; D002402:Cathepsin D
Oznaczanie wolnej aktywności katepsyny D w homogenatach tkankowych po precypitacji organelli komórkowych w pH 5.0.