Genetic toxicity of six carcinogens and six non-carcinogens in the Drosophila wing spot test.
Six rodent carcinogens, 5 of which are also human carcinogens, and 6 compounds recognized as non-carcinogens were tested for their genotoxic activity in the Drosophila melanogaster wing spot test. 72-h-old larvae trans-heterozygous for the recessive wing cell markers 'multiple wing hairs' (mwh) and 'flare' (flr3) were fed various concentrations of the test compounds for a period of 48 h. With amitrole and 4-aminobiphenyl, larvae of the same age were also given an acute treatment of 6 h with higher concentrations, and, in addition, 48-h-old larvae were fed for a longer period of 72 h. Repeats of all experiments document the good reproducibility of the results in the wing spot test. Amitrole and 4-aminobiphenyl were genotoxic after both 48-h and 72-h treatments, but their activity could not be detected following acute exposure of only 6 h. Chlorambucil and melphalan were clearly genotoxic. The carcinogens sodium arsenite and sodium arsenate, however, which are highly toxic to Drosophila, could only be tested at low exposure levels and were negative under these treatment conditions. The 6 non-carcinogens (ascorbic acid, 2-aminobiphenyl, mannitol, piperonyl butoxide, stannous chloride and titanium dioxide) were all definitely non-genotoxic in the Drosophila wing spot test. The data for the non-carcinogens demonstrate that non-genotoxic compounds can be identified in the wing spot test with a reasonable experimental effort.
Tripathy
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Institute of Toxicology, Swiss Federal Institute of Technology, Schwerzenbach.
Würgler
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Frei
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D000611:Aminobiphenyl Compounds / Q000633:toxicity; D000640:Amitrole / Q000633:toxicity; D000818:Animals; D001149:Arsenates / Q000633:toxicity; D001151:Arsenic / Q000633:toxicity; D018053:Arsenites; D002273:Carcinogens / Q000633:toxicity*; D002699:Chlorambucil / Q000633:toxicity; D004331:Drosophila melanogaster / Q000235:genetics*; D005808:Genes, Recessive; D008558:Melphalan / Q000633:toxicity; D009152:Mutagenicity Tests; D009153:Mutagens; D010641:Phenotype; D017670:Sodium Compounds
D000611:Aminobiphenyl Compounds; D001149:Arsenates; D018053:Arsenites; D002273:Carcinogens; D009153:Mutagens; D017670:Sodium Compounds; C006757:4-biphenylamine; D002699:Chlorambucil; C017947:sodium arsenite; C009277:sodium arsenate; D001151:Arsenic; D008558:Melphalan; D000640:Amitrole