Phosphofructokinase during haemopoiesis of the rainbow trout ("Salmo gairdneri R."): its isoenzymatic forms.
Phosphofructokinase (EC 2.7.1.11) from trout haemopoietic cells and erythrocytes exhibits biphasic behaviour, with respect to fructose 6-phosphate and MgATP in extracts filtered through Sephadex G-25. Two different values of Hill coefficient and S0.5 have been found for each cellular population. Two forms of the enzyme with high and low affinity for the substrates, were obtained after affinity chromatography in each cellular population. The kinetic behaviour of these forms is different and may be due to a distinct composition and/or proportion and contribution of phosphofructokinase isoenzymes in both types of cells.
Gaitán
S
S
Departamento de Bioquímica y Biología Molecular IV, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Ruiz-Amil
M
M
Tejero
C
C
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005636:Fructosephosphates / Q000378:metabolism; D006410:Hematopoiesis; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D010732:Phosphofructokinase-1 / Q000378:metabolism*; D014337:Trout / Q000378:metabolism*
D005636:Fructosephosphates; D007527:Isoenzymes; C027618:fructose-6-phosphate; D000255:Adenosine Triphosphate; D010732:Phosphofructokinase-1