[Evaluation of a new colorimetric test (VIP) to determine the presence of lactobacillus in saliva].
This techniques using Rogosa agar modificated with green of bromocresol, placed into tubes (10 ml) containing 4 ml, was developed for qualitative estimation in saliva of lactobacilli in a quick form. We spotted with a pipette 0.1 ml of saliva dilution into different tubes with the culture medium previously mentioned. The tubes were incubated at 37 degrees C for 24 and 48 hours. The results obtained showed a great agreement with the number of latobacilli in the agar.
Yorio
V P
VP
Facultad de Odontología, Montevideo, Uruguay.
Zinemanas
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D002648:Child; D002675:Child, Preschool; D003124:Colorimetry; D003408:Cresols; D003732:Dental Caries Activity Tests; D006801:Humans; D007778:Lactobacillus / Q000302:isolation & purification*; D012463:Saliva / Q000382:microbiology*
D003408:Cresols
Evaluación de un nuevo test colorimétrico (VIP) para determinar la presencia de lactobacilos en saliva.