Microtubule translocation caused by three subspecies of inner-arm dynein from Chlamydomonas flagella.
To help understand the function of inner-arm dynein in flagellar motility, dynein samples from an outer arm-missing mutant of Chlamydomonas (oda1) were examined for the ability to translocate microtubules in vitro. High-salt extract of axonemes containing inner-arm dynein was separated by ion-exchange chromatography into 7 peak fractions with ATPase activities. Of these, three fractions containing different sets of dynein heavy chains translocated microtubules. The maximal velocities were all between 3 and 5 microns/s, which were comparable to the microtubule sliding rate in disintegrating oda axonemes.
Kagami
O
O
Department of Molecular Biology, School of Science, Nagoya University, Japan.
Takada
S
S
Kamiya
R
R
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D002696:Chlamydomonas / Q000648:ultrastructure*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004398:Dyneins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005407:Flagella / Q000648:ultrastructure*; D008870:Microtubules / Q000378:metabolism*
D000251:Adenosine Triphosphatases; D004398:Dyneins