Expression of RNA transcripts of potato virus X full-length and subgenomic cDNAs.
Translation of synthetic potato virus X (PVX) RNAs was examined in Krebs II ascite cell extracts and rabbit reticulocyte lysates. Either full-length or subgenomic RNAs produced by in vitro transcription of cDNAs cloned in the T7 promoter vectors were used. Full-length PVX RNA-transcript directed the synthesis of a large polypeptide which was indistinguishable from the translation product (165 kDa) of authentic viral genomic RNA on SDS-PAGE. Translation of the full-length and 3'-truncated RNA species was stimulated by addition of m7GpppG to the T7 transcription system. Subgenomic cDNA transcripts corresponding to the 3'-proximal part of PVX RNA were efficiently translated into the PVX coat protein and 2 as yet unidentified small hydrophobic proteins.
Morozov
S Y
SY
AN Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.
Miroshnichenko
N A
NA
Zelenina
D A
DA
Fedorkin
O N
ON
Solovijev
A G
AG
Lukasheva
L I
LI
Atabekov
J C
JC
D000818:Animals; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000096:biosynthesis*; D005814:Genes, Viral; D009029:Mosaic Viruses / Q000235:genetics*; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D012315:RNA Caps / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism
D036022:Capsid Proteins; D004279:DNA, Viral; D012315:RNA Caps; D012367:RNA, Viral; C058750:coat protein, Potato virus X