[Production of pyoverdin by Pseudomonas fluorescens in human blood at 4 degrees C: association with proteins and the cytotoxic effect of the pigment].
Pyoverdin was purified from Pseudomonas fluorescens cultured in a synthetic medium. Cytotoxic effect on human leukocytes was assayed. Death and lysis was observed depending on concentration and time. Sub-lytic dose decreased leukocyte phagocytosis. The pigment was produced by P. fluorescens in blood stored at 4 degrees C. Saline precipitation of plasma showed that globulin and albumin fraction retained 46% and 37% of pyoverdin, respectively. By means of dialysis it was possible to determine that albumin retained more pigment than the other fractions either bound to the protein or aggregated.
Pájaro
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MC
Universidad Nacional de Río Cuarto, Departmento de Microbiología e Inmunología, Río Cuarto, Provincia de Córdoba.
Albesa
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I
D001769:Blood / Q000382:microbiology; D001798:Blood Proteins / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D007962:Leukocytes / Q000187:drug effects*; D009842:Oligopeptides; D010587:Phagocytosis / Q000187:drug effects; D010860:Pigments, Biological / Q000096:biosynthesis* / Q000494:pharmacology; D011551:Pseudomonas fluorescens / Q000378:metabolism*; D013696:Temperature
D001798:Blood Proteins; D009842:Oligopeptides; D010860:Pigments, Biological; C042453:pyoverdin
Producción de pioverdina por Pseudomonas fluorescens en sangre humana a 4 grados C: asociación a proteínas y efecto citotóxico del pigmento.