Measurement of PGH synthase activity using a pan-specific monoclonal antibody.
A pan-specific monoclonal antibody that recognizes a variety of prostaglandin moieties and does not recognize arachidonic acid or the hydroxy-eicosatetraenoic acids was used to assess the general ability of a tissue to produce prostaglandins. Although this assay does not give a quantitative measure of PGH synthase activity, it does provide a sensitive and convenient means of screening a large number of samples for prostaglandin production.
Hall
E R
ER
Department of Internal Medicine and Pharmacology, University of Texas Medical School, Houston.
Linthicum
D S
DS
Townsend
G
G
Patterson
B J
BJ
Frasier-Scott
K F
KF
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002417:Cattle; D003429:Cross Reactions; D008168:Lung / Q000201:enzymology; D008861:Microsomes / Q000201:enzymology; D011451:Prostaglandin-Endoperoxide Synthases / Q000032:analysis* / Q000276:immunology; D011453:Prostaglandins / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods
D000911:Antibodies, Monoclonal; D011453:Prostaglandins; D011451:Prostaglandin-Endoperoxide Synthases