title: 17 Beta-hydroxysteroid UDP-glucuronosyl transferase is expressed in bile ductular epithelial cells under physiological conditions.
abstract: Using an improved procedure to isolate pure bile ductular epithelial cells from rat liver, we were able to define UDP-glucuronosyl transferase (UDPGT) isozymes expressed in these cells under physiological conditions. The cells contained mRNA for a 17 beta-hydroxysteroid, a 3 alpha-hydroxysteroid and a phenol UDPGT. Concomitantly, a distinct pattern of UDPGT-immunoreactive proteins was expressed, including a putative 17 beta-hydroxysteroid UDPGT. The presence of this UDPGT isozyme was confirmed by a high level of testosterone glucuronidation activity. This is the first demonstration of a metabolic pathway in bile ductular epithelial cells that is primarily dedicated to the processing of endogenous compounds.
authors: University of Zürich, Institute of Pharmacology and Biochemistry, Switzerland.
authors: K L
authors: KL
authors: Franson
authors: B
authors: B
authors: Burchell
authors: G A
authors: GA
authors: Mathis
mesh_terms: D000818:Animals; D001652:Bile Ducts / Q000166:cytology / Q000201:enzymology*; D004848:Epithelium / Q000201:enzymology; D014453:Glucuronosyltransferase / Q000096:biosynthesis*; D006914:Hydroxysteroids / Q000378:metabolism*; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D007668:Kidney / Q000201:enzymology; D010750:Phosphoproteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000378:metabolism
chemical_list: D006914:Hydroxysteroids; D007527:Isoenzymes; D010750:Phosphoproteins; D012333:RNA, Messenger; D013739:Testosterone; D014453:Glucuronosyltransferase