Regulation of IgG production by suppressor Fc gamma RII+ T hybridomas.
In this work, we analyzed the immunoglobulin heavy (H) and light (L) chain production by two variant B hybridomas, UN2.C3 and UN2.C17.K1 co-cultured with cells from a Fc gamma RII+, IgG-binding factor (IgG-BF)-producer T hybridoma (T2D4.C1) or with cells of a Fc gamma RII-, IgG-BF-nonproducer variant (D10C5). We showed that only the Fc gamma RII+ hybridoma directly inhibits the IgG secretion by UN2.C3 through a soluble mediator. This inhibition affects the H and L chain synthesis as well as the H and L chain-encoding mRNA steady state. No apparent cytotoxic effect could be detected. In contrast, the production of kappa chain by an H chain-negative variant (UN2.C17.K1) was unaffected. This indicates that a complete IgG molecule is required to observe the inhibitory effect induced by T2D4.C1. The pattern of effector/target cell interactions observed in our work suggests that the soluble factor involved in the suppression of IgG production is IgG-BF, able to transiently modify the IgG gene expression in target cells.
Brunati
S
S
Unité INSERM 255, Institut Curie, Paris, France.
Moncuit
J
J
Fridman
W H
WH
Teillaud
J L
JL
D000818:Animals; D000917:Antibody Formation; D000943:Antigens, Differentiation / Q000276:immunology*; D015870:Gene Expression; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000235:genetics; D007142:Immunoglobulin gamma-Chains / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D066298:In Vitro Techniques; D008222:Lymphokines / Q000502:physiology*; D051379:Mice; D029584:Prostatic Secretory Proteins; D012333:RNA, Messenger / Q000235:genetics; D011961:Receptors, Fc / Q000276:immunology*; D017452:Receptors, IgG; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D000943:Antigens, Differentiation; D007074:Immunoglobulin G; D007142:Immunoglobulin gamma-Chains; D007145:Immunoglobulin kappa-Chains; D008222:Lymphokines; D029584:Prostatic Secretory Proteins; D012333:RNA, Messenger; D011961:Receptors, Fc; D017452:Receptors, IgG; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors