Expression of v-fms and c-fms in the hemopoietic cell line FDC-P1.
A hemopoietic cell line FDC-P1 that requires either IL-3 or GM-CSF to survive and proliferate was infected with retroviruses that expressed either c-fms, which encodes the receptor for M-CSF, or v-fms, which is an oncogenic derivative of c-fms. The expression of c-fms allowed FDC-P1 to grow in the absence of IL-3 or GM-CSF provided that M-CSF was present. The M-CSF did not, however, induce macrophage differentiation. The expression of v-fms allowed FDC-P1 to grow in the absence of any added hemopoietic growth factors, including M-CSF, although the addition of M-CSF enhanced v-fms activity. V-fms cell lines grew to a higher cell density in suspension and were tumorigenic.
Dibb
N J
NJ
Laboratory of Molecular Biology, Cambridge, England.
Green
S M
SM
Ralph
P
P
D000818:Animals; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D015870:Gene Expression; D005822:Genetic Vectors; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000378:metabolism*; D007377:Interleukin-3 / Q000494:pharmacology; D016173:Macrophage Colony-Stimulating Factor; D008969:Molecular Sequence Data; D016281:Oncogene Protein gp140(v-fms); D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D016186:Receptor, Macrophage Colony-Stimulating Factor; D011994:Recombinant Proteins / Q000096:biosynthesis; D012190:Retroviridae; D015682:Retroviridae Proteins, Oncogenic / Q000096:biosynthesis*; D014162:Transfection
D003115:Colony-Stimulating Factors; D007377:Interleukin-3; D016281:Oncogene Protein gp140(v-fms); D011518:Proto-Oncogene Proteins; D011994:Recombinant Proteins; D015682:Retroviridae Proteins, Oncogenic; D016173:Macrophage Colony-Stimulating Factor; D016186:Receptor, Macrophage Colony-Stimulating Factor