Tectospinal neurons in hamster contain glutamate-like immunoreactivity.
Immunocytochemistry with a monoclonal antibody directed against glutamate revealed numerous immunoreactive cells in the hamster's superior colliculus (SC). A large number of these neurons were located in the deep layers and many were in the stratum album intermedium (SAI). These neurons appeared similar to the large multipolar cells that have been shown to project to the cervical spinal cord in this species. The combination of retrograde tracing using either Fluoro-Gold- or FITC-labelled latex beads with immunocytochemistry for glutamate revealed that many of the immunoreactive cells did, in fact, project into the predorsal bundle and extend axons as far as the cervical spinal cord.
Mooney
R D
RD
Department of Anatomy, Medical College of Ohio, Toledo 43699.
Bennett-Clarke
C A
CA
King
T D
TD
Rhoades
R W
RW
D000818:Animals; D006224:Cricetinae; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005971:Glutamates / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D009474:Neurons / Q000378:metabolism*; D010477:Perfusion; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*; D013266:Stilbamidines; D013477:Superior Colliculi / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D013861:Thiocyanates
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005971:Glutamates; D013266:Stilbamidines; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate