title: The Drosophila melanogaster z600 gene encodes a chromatin-associated protein synthesized in the syncytial blastoderm.
abstract: The Drosophila melanogaster z600 gene is zygotically expressed with gene transcripts accumulating transiently during early embryogenesis. Based on nucleotide sequence analysis, z600 is predicted to encode a small, basic, histone-like protein. Antibodies generated against a beta-galactosidase::z600 fusion protein immunoprecipitated the z600 in vitro translation product and detected a z600 protein present predominantly in 2- to 4-h embryos. We localized the z600 protein in whole-amount embryos by indirect immunofluorescence. These studies show that the protein is located in the nucleus and associated with chromatin in the syncytial blastoderm.
authors: Department of Biochemistry and Molecular Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
authors: S
authors: S
authors: Galewsky
authors: X L
authors: XL
authors: Xie
authors: R A
authors: RA
authors: Schulz
mesh_terms: D000818:Animals; D000906:Antibodies; D001756:Blastoderm / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D005796:Genes; D009687:Nuclear Proteins / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D014158:Transcription, Genetic
chemical_list: D000906:Antibodies; D002843:Chromatin; D009687:Nuclear Proteins