Production of lambda-phi 29 phage chimeras.
Proheads of bacteriophage lambda which carry the connector of phage phi 29 instead of that of lambda have been produced in vitro. These hybrid proheads have a structure similar to that of normal lambda proheads. Furthermore, the chimeric proheads can package both lambda and phi 29 DNA. These data show that the connector domains involved in both head assembly and DNA packaging are functionally similar. The DNA-containing lambda-phi 29 proheads can be complemented in vitro with phi 29 tails to yield infective particles capable of DNA transfer.
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Centro de Biología Molecular (CSIC-UAM), Facultad de Ciencias, Universidad Autónoma, Madrid, Spain.
Murialdo
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Carrascosa
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D010582:Bacteriophage lambda / Q000235:genetics*; D002213:Capsid / Q000235:genetics; D002678:Chimera; D003090:Coliphages / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D005816:Genetic Complementation Test; D009024:Morphogenesis; D015678:Viral Structural Proteins / Q000235:genetics; D014779:Virus Replication
D004279:DNA, Viral; D015678:Viral Structural Proteins