Direct selenium determination in human whole blood by graphite furnace atomic absorption spectrometry with deuterium correction using a L'vov-platform, a Pd/Mg matrix modification and appropriate dilution.
A mixture of palladium/magnesium nitrate was used as matrix modifier in graphite furnace atomic absorption spectrometry for the direct determination of selenium in human whole blood Addition of HCl up to 0.2 M, a twenty-five fold diluted sample and an appropriate furnace program are necessary to eliminate the interference of iron. Reproducibility of less than 3% at the 95 micrograms/L level, a detection limit of 10 micrograms/L and good accuracy, as determined by intercomparison runs and recovery of spiked samples, are obtained. The proposed method can be used for analysing 4 blood samples per hour.
Van Cauwenbergh
R
R
University of Antwerp (UIA), Department of Pharmaceutical Sciences, Belgium.
Robberecht
H
H
Van Dael
P
P
Deelstra
H
H
D003903:Deuterium; D005069:Evaluation Studies as Topic; D006801:Humans; D007201:Indicator Dilution Techniques; D007501:Iron / Q000097:blood; D015203:Reproducibility of Results; D012643:Selenium / Q000097:blood*; D013054:Spectrophotometry, Atomic / Q000295:instrumentation / Q000379:methods*
D003903:Deuterium; D007501:Iron; D012643:Selenium