title: Semiautomated preparation of DNA templates for large-scale sequencing projects.
abstract: The rate limiting step in a large-scale sequencing project is the generation of single-stranded DNA templates. We describe a fast, semiautomated procedure, using 96-well microtitre plates, in which 192 templates can be readily prepared in 1 day. The technique can be carried out manually or can be semiautomated using a robot pipetting device. We also provide evidence for the reliability and applicability of this method to a large-scale sequencing project.
authors: Medical Research Council Laboratory of Molecular Biology, Cambridge.
authors: V
authors: V
authors: Smith
authors: C M
authors: CM
authors: Brown
authors: A T
authors: AT
authors: Bankier
authors: B G
authors: BG
authors: Barrell
mesh_terms: D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D004279:DNA, Viral / Q000302:isolation & purification*; D004926:Escherichia coli / Q000235:genetics; D005821:Genetic Techniques; D012371:Robotics; D013698:Templates, Genetic
chemical_list: D004279:DNA, Viral