Reovirus type 3 binds to antagonist domains of the beta-adrenergic receptor.
Reovirus type 3 interfered with the binding of beta-adrenergic antagonist ligands to receptors on Y1 adrenal, C6 glioma, and mouse L cells. This inhibition of beta-adrenergic binding was dose related. Reovirus did not interfere with dopaminergic binding or isoproterenol-induced activation of adenylate cyclase. In addition, reovirus infected Y1 cells, which bind beta-adrenergic antagonist ligands but lack agonist-induced activity. These results suggest that reovirus infection is initiated by binding to antagonist (nonfunctional) domains of the adrenergic receptor complex.
Donta
S T
ST
Department of Medicine, University of Connecticut School of Medicine, Farmington 06032.
Shanley
J D
JD
D000310:Adrenal Gland Neoplasms; D000818:Animals; D001667:Binding, Competitive; D004082:Dihydroalprenolol / Q000378:metabolism; D005910:Glioma; D007545:Isoproterenol / Q000494:pharmacology; D007700:Kinetics; D007739:L Cells / Q000378:metabolism; D012089:Mammalian orthoreovirus 3 / Q000502:physiology*; D051379:Mice; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000378:metabolism*; D012087:Reoviridae / Q000502:physiology*; D013134:Spiperone / Q000378:metabolism
D011943:Receptors, Adrenergic, beta; D013134:Spiperone; D004082:Dihydroalprenolol; D007545:Isoproterenol