Sequential release of antigens from chloroform-treated Staphylococcus epidermidis: application towards a possible vaccine.
This study describes the properties of two potential Staphylococcus epidermidis vaccines prepared by chloroform treatment of bacteria and release of antigen from these chloroform-treated organisms. Both vaccines were antigenic on testing with homologous hyperimmune serum and induced immune reactivity in immunized rabbits. There was protective efficacy in mice against intraperitoneal challenge by Staph. epidermidis.
Ahmad
A
A
Department of Medical Microbiology, Medical School, University of Birmingham, Edgbaston, UK.
Clarke
S
S
Buchan
A
A
Skinner
G R
GR
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification*; D001428:Bacterial Vaccines / Q000276:immunology*; D002725:Chloroform / Q000494:pharmacology; D004269:DNA, Bacterial / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D005779:Immunodiffusion; D051379:Mice; D008808:Mice, Inbred CBA; D011817:Rabbits; D013203:Staphylococcal Infections / Q000276:immunology / Q000517:prevention & control*; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000235:genetics / Q000276:immunology*; D013696:Temperature
D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D004269:DNA, Bacterial; D007106:Immune Sera; D002725:Chloroform