[Expression of gene coding for the NS1 protein of the tick-borne encephalitis virus in Escherichia coli cell].
Two possible forms of tick-borne encephalitis virus (TBE) gene NS1 (called NS1' and NS1) were constructed using two overlapping cDNA-fragments of TBE genome and synthetic DNA fragments. This genes were expressed in E. coli cells in expression vector pUR290 as individual proteins or fusion with bacterial beta-galactosidase. The proteins NS1 (Mw. 39 kDa), beta-galactosidase-NS1' (Mw. 162 kDa) and beta-galactosidase-NS1 (Mw. 155 kDa) were effectively synthesized under the Plgc-promoter induction conditions. Expression of NS1' gene results in the formation of two virus-specific proteins (Mw. 46 and 44 kDa). All bacterial analogs of NS1 protein fixed monoclonal and polyclonal antibodies specific to viral NS1.
Pugachev
K V
KV
Pletnev
A G
AG
D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004669:Encephalitis Viruses, Tick-Borne / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D015967:Gene Expression Regulation, Viral; D008969:Molecular Sequence Data; D009876:Operon; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014758:Viral Core Proteins / Q000235:genetics*; D017361:Viral Nonstructural Proteins
D004279:DNA, Viral; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins
Ekspressiia v kletkakh Escherichia coli gena belka NS1 virusa kleshchevogo éntsefalita.