Mapping of the HLA-D region by pulsed-field gel electrophoresis: size variation in the DQ-DR interval.
Pulsed field gel electrophoresis (PFGE) was used to construct genomic maps of different HLA-D region haplotypes. In the course of these experiments a new HLA class II beta chain sequence, DV beta, was located close to DX alpha between DX alpha and DQ beta. All other markers studied, from the DP, DZ/DO, DQ and DR subregions, were consistent in their positions in the haplotypes studied but the distance between DQ alpha and DR alpha differed in various haplotypes. For example, in DR7 (MANN) this distance was approximately 380 kb, but in the DR2 haplotype (AKIBA), it was 270 kb. This may be due to variations, either in the number of duplicated DRB genes or in the length of other uncharted DNA, in the HLA-D region.
Inoko
H
H
Department of Transplantation, School of Medicine, Tokai University, Kanagawa, Japan.
Ando
A
A
Tsuji
K
K
D015342:DNA Probes; D016521:Electrophoresis, Gel, Pulsed-Field; D005796:Genes; D006681:HLA-D Antigens / Q000235:genetics*; D006683:HLA-DQ Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping
D015342:DNA Probes; D006681:HLA-D Antigens; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens