Mutation in a heterochromatin-specific chromosomal protein is associated with suppression of position-effect variegation in Drosophila melanogaster.
We report here that a point mutation in the gene which encodes the heterochromatin-specific nonhistone chromosomal protein HP-1 in Drosophila melanogaster is associated with dominant suppression of position-effect variegation. The mutation, a G-to-A transition at the first nucleotide of the last intron, causes missplicing of the HP-1 mRNA. This suggests that heterochromatin-specific proteins play a central role in the gene suppression associated with heterochromatic position effects.
Eissenberg
J C
JC
E. A. Doisy Department of Biochemistry and Molecular Biology, St. Louis University School of Medicine, MO 63104.
James
T C
TC
Foster-Hartnett
D M
DM
Hartnett
T
T
Ngan
V
V
Elgin
S C
SC
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D000090266:Chromobox Protein Homolog 5; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004331:Drosophila melanogaster / Q000235:genetics*; D015723:Gene Library; D006570:Heterochromatin / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D013489:Suppression, Genetic
D002868:Chromosomal Proteins, Non-Histone; D006570:Heterochromatin; D015345:Oligonucleotide Probes; D000090266:Chromobox Protein Homolog 5; D004247:DNA