title: 31P nuclear magnetic resonance (NMR) identification of sugar phosphates in isolated rat ovarian follicular granulosa cells and the effects of follicle-stimulating hormone.
abstract: Immature female rats (23-30 days old) were implanted subcutaneously with diethylstilbestrol (DES) in silastic capsules. After 48 h their ovaries were removed and the granulosa cells isolated (Foreman et al. (1984) Life Sci. 35, 1273-1279). The cells were incubated in Hepes balanced saline buffer with substrates with or without follicle-stimulating hormone (FSH). At the end of incubation perchloric acid extracts were made for 31P NMR spectroscopy. The resonances of fructose 1-phosphate, fructose 6-phosphate, glucose 1-phosphate, and ribose 5-phosphate were identified in the granulosa cell extracts. The relative intensities of fructose 6-phosphate to ribose 5-phosphate decreased after incubation with FSH in vitro. This suggests that FSH increases the activity of the pentose pathway within 1 h. Thus, FSH can acutely activate those metabolic pathways which provide nicotinamide-adenine dinucleotide phosphate (NADPH) to be used in steroid synthesis and cholesterol mobilization.
authors: Department of Biology, Case Western Reserve University, Cleveland, OH 44106.
authors: J D
authors: JD
authors: Bentley
authors: J E
authors: JE
authors: Jentoft
authors: D
authors: D
authors: Foreman
authors: D
authors: D
authors: Ambrose
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006107:Granulosa Cells / Q000737:chemistry* / Q000187:drug effects; D006600:Hexosephosphates / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D009249:NADP / Q000096:biosynthesis; D010052:Ovariectomy; D010427:Pentose Phosphate Pathway; D010761:Phosphorus Radioisotopes; D051381:Rats
chemical_list: D006600:Hexosephosphates; D010761:Phosphorus Radioisotopes; D009249:NADP; D005640:Follicle Stimulating Hormone; D002784:Cholesterol