Zymodeme conversion of isolates of Entamoeba histolytica.
A non-cloned and a cloned isolate of non-pathogenic (zymodeme I) Entamoeba histolytica derived from homosexual males were incubated in Diamonds' TP-S-1 medium in the presence of Crithidia for 52 d. A single addition of Escherichia coli irradiated at 1000 Gy rendered the culture axenic. Analysis of zymodeme patterns established that both isolates had transformed to zymodeme II. The re-establishment of cultures in the presence of microflora from the original cultures resulted in a return to zymodeme I, whereas incubation with microflora from an amoebic isolate exhibiting a zymodeme II pattern maintained the pathogenic pattern.
Andrews
B J
BJ
Institute of International Health, University of Bergen, Haukeland Hospital, Norway.
Mentzoni
L
L
Bjorvatn
B
B
D000818:Animals; D003421:Crithidia; D003470:Culture Media; D004748:Entamoeba histolytica / Q000145:classification / Q000201:enzymology* / Q000254:growth & development; D005956:Glucose-6-Phosphate Isomerase / Q000032:analysis; D006593:Hexokinase / Q000032:analysis; D007527:Isoenzymes / Q000032:analysis*; D008291:Malate Dehydrogenase / Q000032:analysis; D010733:Phosphoglucomutase / Q000032:analysis
D003470:Culture Media; D007527:Isoenzymes; D008291:Malate Dehydrogenase; C031917:malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+); D006593:Hexokinase; D005956:Glucose-6-Phosphate Isomerase; D010733:Phosphoglucomutase