Identification of 2-(2'-octenyl) succinic acid in urine.
2-(2'-octenyl)succinic acid has been identified in urine samples from children investigated for a possible inherited metabolic disease. Its structural identification has been achieved by gas chromatography/mass spectrometry using both electron ionization and chemical ionization and by tandem mass spectrometry (MS/MS) using fast-atom bombardment and high-resolution electron-ionization analyses of the molecular ion in a complex biological matrix. The localization of the double bond was obtained by interpretation of a unexpected rearrangement reaction occurring after dimethyl disulfide derivatization.
Giordano
G
G
Mass Spectrometry Facility, Yale University School of Medicine, New Haven, CT.
McMurray
W J
WJ
Previs
S F
SF
Welch
R D
RD
Rinaldo
P
P
D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007223:Infant; D008661:Metabolism, Inborn Errors / Q000652:urine*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013386:Succinates / Q000652:urine*
D013386:Succinates; C073722:2-(2'-octenyl)succinic acid