High-performance liquid chromatographic analysis of doxacurium, a new long-acting neuromuscular blocker.
A rapid and sensitive high-performance liquid chromatographic procedure was developed for the analysis of the new, long-acting neuromuscular blocker doxacurium in the plasma and urine of dog and man and in the bile of dog. Samples were prepared on solid-phase extraction cartridges containing a methyl (C1) bonded phase and were chromatographed on a 15 cm reversed-phase column (C1) using a mobile phase of 0.05 M monobasic potassium phosphate-acetonitrile (30:70, v/v). The compound was detected at 210 nm with a lower limit of quantitation of 10 ng/ml. An inter-assay accuracy of 90-92% was obtained for the analysis of the drug from biological fluids. The method was applied to studies of doxacurium after intravenous administration to dog and man.
DeAngelis
R
R
Department of Medicinal Biochemistry, Burroughs Wellcome Co., Durham, NC 27713.
Loebs
P
P
Maehr
R
R
Savarese
J
J
Welch
R
R
D000818:Animals; D001646:Bile / Q000032:analysis; D001798:Blood Proteins / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D004355:Drug Stability; D006801:Humans; D007546:Isoquinolines / Q000032:analysis* / Q000097:blood / Q000652:urine; D007700:Kinetics; D003473:Neuromuscular Nondepolarizing Agents; D011485:Protein Binding; D011786:Quality Control
D001798:Blood Proteins; D007546:Isoquinolines; D003473:Neuromuscular Nondepolarizing Agents; C049429:doxacurium