Rat pancreatic colipase mRNA: nucleotide sequence of a cDNA clone and nutritional regulation by a lipidic diet.
A cDNA clone encoding rat pancreatic colipase was isolated using as a probe a synthetic deoxyoligonucleotide corresponding to a highly conserved amino acid sequence region in colipases from other species. The cloned messenger codes for a protein of 95 amino acids plus a signal peptide of 17 amino acids. The structure of the full-length cDNA was also determined and the corresponding amino acid sequence showed a high degree of homology with those of other known colipases. Quantification of the homologous mRNA in the pancreas of animals fed a high-lipid diet was consistent with a specific though moderate induction of colipase messenger by the nutritional manipulation.
Wicker
C
C
Centre de Biochimie et de Biologie Moléculaire du Centre National de la Recherche Scientifique, Marseille, France.
Puigserver
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D002999:Clone Cells; D003089:Colipases / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D004041:Dietary Fats / Q000008:administration & dosage*; D006736:Horses; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology*; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D012689:Sequence Homology, Nucleic Acid; D013552:Swine
D003089:Colipases; D004041:Dietary Fats; D011506:Proteins; D012333:RNA, Messenger; D004247:DNA