title: Expression of RNA transcripts of potato virus X full-length and subgenomic cDNAs.
abstract: Translation of synthetic potato virus X (PVX) RNAs was examined in Krebs II ascite cell extracts and rabbit reticulocyte lysates. Either full-length or subgenomic RNAs produced by in vitro transcription of cDNAs cloned in the T7 promoter vectors were used. Full-length PVX RNA-transcript directed the synthesis of a large polypeptide which was indistinguishable from the translation product (165 kDa) of authentic viral genomic RNA on SDS-PAGE. Translation of the full-length and 3'-truncated RNA species was stimulated by addition of m7GpppG to the T7 transcription system. Subgenomic cDNA transcripts corresponding to the 3'-proximal part of PVX RNA were efficiently translated into the PVX coat protein and 2 as yet unidentified small hydrophobic proteins.
authors: AN Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.
authors: S Y
authors: SY
authors: Morozov
authors: N A
authors: NA
authors: Miroshnichenko
authors: D A
authors: DA
authors: Zelenina
authors: O N
authors: ON
authors: Fedorkin
authors: A G
authors: AG
authors: Solovijev
authors: L I
authors: LI
authors: Lukasheva
authors: J C
authors: JC
authors: Atabekov
mesh_terms: D000818:Animals; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000096:biosynthesis*; D005814:Genes, Viral; D009029:Mosaic Viruses / Q000235:genetics*; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D012315:RNA Caps / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral; D012315:RNA Caps; D012367:RNA, Viral; C058750:coat protein, Potato virus X