Identification and partial purification of soluble antigens from culture-grown Besnoitia besnoiti endozoites.
Soluble antigens from culture-grown Besnoitia besnoiti endozoites were identified following their partial purification by affinity chromatography. A specific eluate obtained after affinity chromatography on a column to which antibodies from serum of a naturally infected cow were bound exhibited seven polypeptide bands on sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE). Five bands were observed in the eluate from an immunoadsorbent to which antibodies from an experimentally infected calf were coupled. Eluted antigens were reactive in the enzyme-linked immunosorbent assay (ELISA). Reactivity of electrophoretically resolved antigens with sera of endozoite-inoculated cattle and sera from field cases of besnoitiosis were studied using the Western immunoblotting technique.
Shkap
V
V
Kimron Veterinary Institute, Beit Dagan, Israel.
Ungar-Waron
H
H
Pipano
E
E
D000818:Animals; D000953:Antigens, Protozoan / Q000302:isolation & purification*; D016782:Apicomplexa / Q000276:immunology*; D015153:Blotting, Western; D002417:Cattle; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay
D000953:Antigens, Protozoan