title: Role of polyamines in the early adaptive response to jejunectomy in the rat: effect of DFMO on the ileal villus:crypt axis.
abstract: To study the role of the polyamines putrescine, spermidine and spermine and of the enzymes controlling their synthesis (ornithine decarboxylase; ODC) and degradation (diamine oxidase; DAO) along the villus:crypt axis at the crucial early stage of the ileal adaptive response to jejunectomy, we measured polyamine concentrations and the activities of ODC, DAO and alkaline phosphatase (a marker of enterocyte maturity) in epithelial cells isolated by the Weiser technique from villus tips, mid villi, lower villi and crypts 4 days after surgery in transected control (TRC) and jejunectomised rats untreated or given the specific ODC blocker, alpha-difluoromethyl ornithine (DFMO, 2% in drinking water beginning 3 days before surgery). In the TRCs, there was a diminishing villus tip-to-crypt gradient not only in alkaline phosphatase but also in ODC and DAO activities. After jejunectomy, there were up to 93% increases in mean enterocyte ODC activity when compared with the corresponding cell fractions from the TRCs, but in both the control and jejunectomised rats, DFMO treatment markedly inhibited ODC activity (p less than 0.05-0.01) and reduced spermidine and particularly putrescine concentrations (p less than 0.005-0.001) in all four cell fractions. Only 4 days post-operation, jejunectomy stimulated a significant increase in ileal wet weight but DFMO treatment completely prevented this adaptive response and significantly reduced segmental intestinal weight (mg/cm) in the TRCs. These results (i) extend our knowledge of polyamines and related enzymes along the villus:crypt gradient in the normal intestine, (ii) provides the first data on these variables after resection, and (iii) lend further support to the hypothesis that changes in enterocyte ODC activity and in putrescine and spermidine concentrations play an important role in initiating the ileal adaptive response to proximal small bowel resection in the rat.
authors: Department of Internal Medicine, Shiga University of Medical Science, Japan.
authors: T
authors: T
authors: Bamba
authors: S
authors: S
authors: Vaja
authors: G M
authors: GM
authors: Murphy
authors: R H
authors: RH
authors: Dowling
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology; D000469:Alkaline Phosphatase / Q000378:metabolism; D006631:Amine Oxidase (Copper-Containing) / Q000502:physiology; D000818:Animals; D015317:Biogenic Polyamines / Q000502:physiology*; D000518:Eflornithine / Q000494:pharmacology*; D006965:Hyperplasia; D007082:Ileum / Q000473:pathology*; D007413:Intestinal Mucosa / Q000473:pathology; D007583:Jejunum / Q000601:surgery*; D008297:Male; D009955:Ornithine Decarboxylase / Q000502:physiology*; D065108:Ornithine Decarboxylase Inhibitors; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D015317:Biogenic Polyamines; D065108:Ornithine Decarboxylase Inhibitors; D006631:Amine Oxidase (Copper-Containing); D000469:Alkaline Phosphatase; D009955:Ornithine Decarboxylase; D000518:Eflornithine