[3H]Harman labels selectively and with high affinity the active site of monoamine oxidase (EC 1.4.3.4) subtype A (MAO-A) in rat, marmoset, and pig.
[3H]Harman was used in binding studies with CNS tissue of rat, pig, and marmoset and with visceral organs of the rat. In the mitochondrial fractions of the CNS of the 3 species [3H]harman binding exhibits the same pharmacological profile in displacement studies. A detailed analysis reveals a high specificity for MAO-A. Furthermore, we applied [3H]harman binding to quantify the MAO-A content in 6 CNS regions of each species as well as in 6 visceral organs of the rat.
May
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Department of Neuropsychopharmacology, Free University, Berlin, Federal Republic of Germany.
Strauss
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Rommelspacher
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D000818:Animals; D001665:Binding Sites; D002143:Callitrichinae; D002540:Cerebral Cortex / Q000201:enzymology; D003521:Cyclopropanes / Q000494:pharmacology; D006247:Harmine / Q000031:analogs & derivatives*; D007700:Kinetics; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D008995:Monoamine Oxidase / Q000378:metabolism*; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D013552:Swine
D003521:Cyclopropanes; D008996:Monoamine Oxidase Inhibitors; D011506:Proteins; D006247:Harmine; C000434:Lilly 51641; C005010:harman; D008995:Monoamine Oxidase