Semicarbazide-sensitive amine oxidase activity in rat aortic cultured smooth muscle cells.
Metabolism of 5 microM benzylamine (BZ) by rat aortic cultured smooth muscle cells (SMC) is inhibited almost completely by 10(-3) M semicarbazide and 10(-6) M propargylamine, but is little affected by 10(-4) M and 10(-3) M pargyline and clorgyline, indicating BZ metabolism predominantly by the semicarbazide-sensitive amine oxidase (SSAO) previously characterized in rat aortic homogenates. Km values of 7-9 microM for BZ metabolism by SSAO in SMC cultures, indicate similar, if not identical properties, to the enzyme in the parent blood vessel.
Blicharski
J R
JR
Department of Pharmacology and Clinical Pharmacology, University of Dundee, Ninewells Hospital and Medical School, United Kingdom.
Lyles
G A
GA
D006631:Amine Oxidase (Copper-Containing) / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology / Q000201:enzymology; D001596:Benzylamines / Q000378:metabolism; D002478:Cells, Cultured; D003010:Clorgyline / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000201:enzymology*; D010293:Pargyline / Q000494:pharmacology; D051381:Rats
D001596:Benzylamines; D008996:Monoamine Oxidase Inhibitors; D010293:Pargyline; C030796:benzylamine; D006631:Amine Oxidase (Copper-Containing); D003010:Clorgyline