Tissue distribution and appearance in ontogeny of alpha/beta T cell receptor (TCR2) in chicken.
We have performed immunoperoxidase staining on cryostat tissue sections and immunofluorescence analysis on cell suspensions to identify cells expressing the alpha/beta T cell antigen receptor during ontogeny and adult life in chickens. We used the mouse monoclonal antibody, TCR2, which was previously shown to recognize the alpha/beta TCR in chickens. TCR2+ cells were observed in thymic cortex and medulla and in T-dependent areas of spleen, intestine, and cecal tonsils of young adult chickens. Some TCR2+ cells were found in the cortex of bursal follicles and in liver. The first TCR2+ cells appear in thymus on Day 13 of the embryonic life and it is only after hatching that TCR2+ cells begin to migrate to the periphery.
Vainio
O
O
Basel Institute for Immunology, Switzerland.
Lassila
O
O
Cihak
J
J
Lösch
U
U
Houssaint
E
E
D000367:Age Factors; D000818:Animals; D002642:Chick Embryo; D002645:Chickens / Q000276:immunology*; D007124:Immunoenzyme Techniques; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D016693:Receptors, Antigen, T-Cell, alpha-beta; D013154:Spleen / Q000196:embryology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D013950:Thymus Gland / Q000196:embryology / Q000276:immunology; D014018:Tissue Distribution
D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta