title: In vitro peptidoglycan synthesis by envelopes from Escherichia coli tolM mutants is inhibited by colicin M.
abstract: An in vitro peptidoglycan synthesis reaction was employed to further characterize the role of the tolM product in colicin M-induced inhibition of peptidoglycan synthesis. It was found that the tolM product is not the colicin M target and that this gene product does not play a role in the interaction of the colicin with its target. Colicin M remained associated with envelopes prepared from colicin-treated tolM mutants. These findings suggested that the tolM product most likely is involved with the internalization of colicin M.
authors: Mikrobiologie II, Universität Tübingen, Federal Republic of Germany.
authors: R E
authors: RE
authors: Harkness
authors: V
authors: V
authors: Braun
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D002118:Calcium / Q000494:pharmacology; D003087:Colicins / Q000378:metabolism / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D005798:Genes, Bacterial; D009154:Mutation; D010457:Peptidoglycan / Q000096:biosynthesis*
chemical_list: D001426:Bacterial Proteins; D003087:Colicins; D010457:Peptidoglycan; D002118:Calcium