Features of the beta-amylase isoform system in dry and germinating seeds of alfalfa (Medicago sativa L.).
Five isoforms of beta-amylase were purified to homogeneity from alfalfa seeds (Medicago sativa L.) by chromatofocusing and cation-exchange chromatography. These isoforms were identified as beta-amylase based on their catalytic mode to the substrates. These isoforms of beta-amylase were also found in germinating seeds of alfalfa. All the isoforms existed in free form, because they could be extracted without reducing agent. The five isoforms had different isoelectric points (5.05, 4.97, 4.85, 4.82 and 4.77), but their Mr was the same (61 kDa) on SDS-polyacrylamide gels. The amino acid compositions were similar, but not identical, to each other. An antiserum raised against one of the five isoforms cross-reacted with all of other isoforms, but did not recognize the component 2 of soybean beta-amylase. The amounts of five isoforms increased during seed germination, which was responsible for significant increase of the beta-amylase activity in germinating seeds.
Kohno
A
A
Department of Agricultural Chemistry, Faculty of Agriculture, Kobe University, Japan.
Shinke
R
R
Nanmori
T
T
D000596:Amino Acids / Q000032:analysis; D002852:Chromatography, Ion Exchange; D007106:Immune Sera; D015151:Immunoblotting; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000302:isolation & purification* / Q000378:metabolism; D000455:Medicago sativa / Q000201:enzymology*; D008970:Molecular Weight; D012639:Seeds / Q000201:enzymology; D001614:beta-Amylase / Q000302:isolation & purification* / Q000378:metabolism
D000596:Amino Acids; D007106:Immune Sera; D007527:Isoenzymes; D001614:beta-Amylase