Short N-terminal deletions in the phage phi 29 transcriptional activator protein impair its DNA-binding ability.
The expression of Bacillus subtilis phage phi 29 late genes from the A3 promoter requires the viral protein p4. This protein is a transcriptional activator which binds to a region of the A3 promoter located between nucleotides -56 to -102, relative to the transcription start point. Mutants at the N terminus of protein p4 have been constructed and their function investigated. The binding of these deletion mutants to the late A3 promoter has been analyzed by gel retardation and DNase I footprinting assays. The results indicate that the N terminus of protein p4 could be involved in its binding to the A3 promoter, suggesting that it may not be a typical Cro-like helix-turn-helix DNA-binding protein.
Rojo
F
F
Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma de Madrid, Spain.
Salas
M
M
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics*; D002872:Chromosome Deletion; D016172:DNA Fingerprinting; D004279:DNA, Viral / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic; D015533:Transcriptional Activation; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; D004268:DNA-Binding Proteins; D014764:Viral Proteins; C061994:p3 protein, bacteriophage phi 29