Functional interaction of nuclear transport-defective simian virus 40 large T antigen with chromatin and nuclear matrix.
We analyzed the subcellular distribution of nuclear transport-defective simian virus 40 Lys-128-mutant (cT-3 [R. E. Lanford and J. S. Butel, Cell 37:801-813, 1984] and d10 [D. Kalderon, W. D. Richardson, A. F. Markham, and A. E. Smith, Nature (London) 311:33-38, 1984]) large T antigens in various Lys-128-mutant-transformed rodent cells and in Lys-128-mutant d10-infected TC7 cells. Small but significant amounts of the mutant large T antigens were found in association with nuclear substructures, both in mutant-transformed and in mutant-infected cells. Experiments with TC7 cells made incompetent for cell division by 60Co irradiation supported the assumption that Lys-128-mutant large T antigen did not associate with nuclear components during mitosis but most likely was transported into the nucleus because the Lys-128 mutation was leaky for nuclear transport. Low-level simian virus 40 DNA replication and production of infectious mutant virus progeny in TC7 cells indicated that the association of Lys-128-mutant large T antigen with nuclear substructures is functional.
Deppert
W
W
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität Hamburg, Federal Republic of Germany.
Von Der Weth
A
A
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000032:analysis / Q000235:genetics*; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism*; D003001:Cloning, Molecular; D004261:DNA Replication / Q000528:radiation effects; D003673:Defective Viruses / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D007700:Kinetics; D009154:Mutation; D015530:Nuclear Matrix / Q000378:metabolism*; D010957:Plasmids; D013539:Simian virus 40 / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D014162:Transfection
D000952:Antigens, Polyomavirus Transforming; D002843:Chromatin