title: Blockage of a pump-related calcium-efflux pathway in light sarcoplasmic reticulum vesicles by Mops.
abstract: Mops, used as a proton buffer, specifically enhances the accumulation of calcium or strontium by light sarcoplasmic reticulum vesicles driven by ATP or dinitrophenylphosphate as energy-yielding substrates when calcium-precipitating agents are absent. The enhancement of ion uptake by Mops is much greater for strontium than for calcium and is further increased when potassium is replaced by sodium as the dominant monovalent cation. Mops affects neither the activity of the calcium- or strontium-activated transport enzyme nor the active accumulation of calcium in the presence of oxalate, i.e. when the pump runs unidirectionally forward. Passive calcium and strontium efflux rates of approximately 40-50 nmol.mg-1.min-1 are considerably reduced when histidine/glycerophosphate or Tris/maleate are exchanged for Mops. The observed passive efflux rates and their modulation by Mops are too small, in relation to the rate of ion influx, to account for either the relatively small calcium and strontium load in the absence of precipitating agents or for its modulation by Mops. The results imply that the pump itself mediates ion efflux dependent on pump activity and the different degree of saturation of lumenal ion-binding sites by calcium and strontium, as well as their susceptibility to Mops.
authors: Max-Planck-Institut für Medizinische Forschung, Abteilung für Physiologie, Heidelberg, Federal Republic of Germany.
authors: S
authors: S
authors: Stephan
authors: A
authors: A
authors: Migala
authors: W
authors: W
authors: Hasselbach
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D004140:Dinitrophenols / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism; D008027:Light; D009025:Morpholines; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*; D013324:Strontium / Q000378:metabolism
chemical_list: D004140:Dinitrophenols; D009025:Morpholines; C008550:morpholinopropane sulfonic acid; D000255:Adenosine Triphosphate; D000252:Calcium-Transporting ATPases; D002118:Calcium; D013324:Strontium