The membrane-bound domain of the phosphotransferase enzyme IImtl of Escherichia coli constitutes a mannitol translocating unit.
The orientation of the mannitol binding site on the Escherichia coli phosphotransferase enzyme IImtl in the unphosphorylated state has been investigated by measuring mannitol binding to cytoplasmic membrane vesicles with a right-side-out and inside-out orientation. Enzyme IImtl is shown to catalyze facilitated diffusion of mannitol at a low rate. At equilibrium, bound mannitol is situated at the periplasmic side of the membrane. The apparent binding constant is 40 nM for the intact membranes. Solubilization of the membranes in detergent decreases the affinity by about a factor of 2. Inside-out membrane vesicles, treated with trypsin to remove the C-terminal cytoplasmic domain of enzyme IImtl, showed identical activities. These experiments indicate that the translocation of mannitol is catalyzed by the membrane-bound N-terminal half of enzyme IImtl which is a structurally stable domain.
Lolkema
J S
JS
Department of Physical Chemistry, Groningen, The Netherlands.
Dijkstra
D S
DS
ten Hoeve-Duurkens
R H
RH
Robillard
G T
GT
D001665:Binding Sites; D002462:Cell Membrane / Q000201:enzymology; D004058:Diffusion; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins; D007700:Kinetics; D008353:Mannitol / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins; D010446:Peptide Fragments / Q000378:metabolism; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000502:physiology*; D013329:Structure-Activity Relationship; D014357:Trypsin
D029968:Escherichia coli Proteins; D009004:Monosaccharide Transport Proteins; D010446:Peptide Fragments; D008353:Mannitol; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C022358:mannitol PTS permease, E coli; D014357:Trypsin