The transmembrane topology of the a [corrected] subunit from the ATPase in Escherichia coli analyzed by PhoA protein fusions.
The atpB encodes the a [corrected] subunit of the H(+)-ATPase of E. coli. The topology of this membrane protein has been analyzed by PhoA fusions. The results support an eight transmembrane segment model that is consistent with the hydropathic profile.
Bjørbaek
C
C
Department of Microbiology, Technical University of Denmark, Lyngby.
Foërsom
V
V
Michelsen
O
O
D000469:Alkaline Phosphatase / Q000032:analysis; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000032:analysis; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D005804:Genes, Lethal; D008565:Membrane Proteins / Q000032:analysis* / Q000235:genetics; D008956:Models, Chemical; D010455:Peptides / Q000032:analysis; D010957:Plasmids; D011487:Protein Conformation; D006180:Proton-Translocating ATPases / Q000032:analysis* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000032:analysis
D008565:Membrane Proteins; D010455:Peptides; D011993:Recombinant Fusion Proteins; D000469:Alkaline Phosphatase; D006180:Proton-Translocating ATPases