Oligo(3'-deoxy ADP-ribosyl)ation of the nuclear matrix lamins from rat liver utilizing 3'-deoxyNAD as a substrate.
It has previously been shown that the levels of poly(ADP-ribose)polymerase and polymers of ADP-ribose that co-purify with the nuclear matrix in regenerating liver fluctuate with the levels of in vivo DNA replication [(1988) FEBS Lett. 236, 362-366]. We have now electrophoretically identified lamins A and C, and poly(ADP-ribose)polymerase as the main protein targets for poly(ADP-ribosyl)ation in isolated nuclear matrices from adult rat liver. The identification of these protein acceptors was facilitated by the utilization of 32P-radiolabeled 3'-deoxyNAD as a substrate for nuclear matrix extracts in the presence of exogenously added DNA-dependent poly(ADP-ribose)polymerase from calf thymus. The extent of protein modification was time- and substrate concentration-dependent. These results are consistent with the hypothesis that the poly(ADP-ribose) modification of the lamins A and C and poly(ADP-ribose)polymerase are important to modulate chromatin-nuclear matrix interactions in rat liver.
Pedraza-Reyes
M
M
Department of Microbiology and Immunology, Texas College of Osteopathic Medicine, Fort Worth 76107-2690.
Alvarez-Gonzalez
R
R
D000818:Animals; D004268:DNA-Binding Proteins / Q000378:metabolism; D066298:In Vitro Techniques; D034882:Lamins; D009243:NAD / Q000378:metabolism* / Q000031:analogs & derivatives; D015530:Nuclear Matrix / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D004268:DNA-Binding Proteins; D034882:Lamins; D009687:Nuclear Proteins; D009243:NAD; D011064:Poly Adenosine Diphosphate Ribose; C057676:3'-deoxynicotinamide adenine dinucleotide; D011065:Poly(ADP-ribose) Polymerases