[Viability of Bacillus subtilis auxotrophs in the absence of their essential metabolites].
The effect of tryptophan and uracil starvation on the viability of the transformant B. subtilis BSA 170 trp- ura- and its parent strains Bacillus subtilis PB 168 trp -C and Bacillus subtilis PB 3308 ura- was examined. These studies were performed at the conditions for competence development, during 16 hours. Our results showed that B. subtilis BSA 170 was resistant to tryptophan-less death during all the assay and was also resistant to uracil-less death during three hours. After this time, viability measurements revealed less colony forming units per milliliter, and decrease of the culture absorbances. The uracil-less death required the presence of tryptophan suggesting that protein synthesis is needed. The parental strains exhibit similar behavior. Bacillus subtilis PB 168 was resistant to tryptophan-less death and B. subtilis PB 3308 showed decrease of the viability after uracil starvation comparable to that of the transformant strain.
Franco
M A
MA
Departamento de Microbiología y e Inmunología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
Santillán
M A
MA
de Torres
R A
RA
D'Aquino
M
M
D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis; D014364:Tryptophan / Q000494:pharmacology; D014498:Uracil / Q000494:pharmacology
D001426:Bacterial Proteins; D014498:Uracil; D014364:Tryptophan
Viabilidad de Bacillus subtilis auxotrofos en ausencia de sus metabolitos esenciales.