Temperature-mediated processes in immunity: differential effects of low temperature on mouse T helper cell responses.
A low culture temperature of 27 degrees C inhibited mouse primary in vitro anti-hapten plaque-forming cell responses to a thymus-dependent (TD) antigen (Ag) (trinitrophenyl-keyhole limpet hemocyanin, TNP-KLH). In contrast, the magnitudes of secondary responses to TNP-KLH or primary responses to a thymus-independent (TI) Ag (TNP-lipopolysaccharide (LPS)) were unaffected. The low-temperature-sensitive step in the primary TD response occurred relatively early and preceded interleukin 2 (IL-2) secretion. Furthermore, the low-temperature-induced suppression could be obviated (rescued) by recombinant IL-2 or IL-4, but not by IL-1. Thus, the low temperature appeared to inhibit the function of virgin Th cells by preferentially affecting T cell-derived interleukin synthesis/secretion and not other cellular activities. These results also imply fundamental differences between the activation requirements of memory and virgin Th cells.
Wang-Yang
M C
MC
Department of Microbiology, University of Mississippi Medical Center, Jackson 39216.
Buttke
T M
TM
Miller
N W
NW
Clem
L W
LW
D000818:Animals; D003080:Cold Temperature; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007375:Interleukin-1 / Q000096:biosynthesis / Q000494:pharmacology; D007376:Interleukin-2 / Q000096:biosynthesis / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology; D013154:Spleen / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects / Q000276:immunology*; D013696:Temperature; D013950:Thymus Gland / Q000276:immunology
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007375:Interleukin-1; D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins