Stimulation of rat red blood cell glycolysis by phenylhydrazine hydrochloride.
The effects of phenylhydrazine hydrochloride (5, 10 and 20 mM) on the energy metabolism of red cells of rats were studied. There were found an 1.3-2-fold increase of lactate formation, an up to seven-fold accumulation of pyruvate and of the flux via the oxidative pentose pathway. The stimulation of the glycolysis was caused by 1) a large decline of the ATP concentration and a corresponding increase of ADP and AMP, and 2) by the stimulation of the actual activities of the Na+,K(+)-ATPase and Ca+(+)-ATPase owing to a greater permeability of the plasma membrane. A significant increase of the Ca+(+)-influx was demonstrated. A decline of the sum of adenine nucleotides after 60 and 90 min of incubation with phenylhydrazine was not accompanied by accumulation of hypoxanthine.
Kostić
M M
MM
Institute of Physiology, Faculty of Medicine, University Svetozar Markovic, Kragujevac, Yugoslavia.
Dragićević
L
L
Zirković
R
R
Müller
M
M
Rapoport
S M
SM
D000227:Adenine Nucleotides / Q000097:blood / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000097:blood / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000097:blood / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006019:Glycolysis / Q000187:drug effects*; D007773:Lactates / Q000097:blood / Q000378:metabolism; D019344:Lactic Acid; D010427:Pentose Phosphate Pathway; D010659:Phenylhydrazines / Q000494:pharmacology*; D011773:Pyruvates / Q000097:blood / Q000378:metabolism; D019289:Pyruvic Acid; D051381:Rats; D011919:Rats, Inbred Strains
D000227:Adenine Nucleotides; D007773:Lactates; D010659:Phenylhydrazines; D011773:Pyruvates; C030299:phenylhydrazine; D019344:Lactic Acid; D019289:Pyruvic Acid; D000252:Calcium-Transporting ATPases; D002118:Calcium