Antibody to integrin alpha 6 subunit specifically inhibits cell-binding to laminin fragment 8.
A large number of cell lines which attach and spread on laminin show a comparable binding either to both laminin fragments P1 and E8 or exclusively to E8. Adhesion to fragment E8 was with one exception completely inhibited by a monoclonal antibody to the alpha 6 integrin subunit, indicating that VLA-6 or a related structure is the major cellular receptor for laminin. It is not involved in fragment P1 adhesion. Synthetic peptides possessing RGD or YIGSR sequences were without inhibitory activity for alpha 6-mediated adhesion to fragment E8.
Aumailley
M
M
Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany.
Timpl
R
R
Sonnenberg
A
A
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology*; D002448:Cell Adhesion / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D006801:Humans; D016023:Integrins / Q000276:immunology*; D007797:Laminin / Q000032:analysis / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D011971:Receptors, Immunologic / Q000378:metabolism*; D017474:Receptors, Laminin; D014407:Tumor Cells, Cultured / Q000378:metabolism
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D016023:Integrins; D007797:Laminin; D011971:Receptors, Immunologic; D017474:Receptors, Laminin