Neutrophil derived interference in the fluorometric determination of histamine.
Zymogen-stimulated neutrophils release many pre- and non-preformed factors including proteolytic enzymes, oxygen free radicals and arachidonic acid metabolites. Measurement of the histamine concentration of a supernatant fluid commonly employs an organic extraction procedure followed by condensation to o-phthalaldehyde. Diamine oxidase digestion studies and high-performance liquid chromatographic analysis suggest that a factor(s) derived from zymogen-stimulated neutrophils can produce a positive interference in the fluorometric determination of histamine.
Siegel
P D
PD
Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health (NIOSH), Morgantown, W.V.
Lewis
D M
DM
Olenchock
S A
SA
D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004792:Enzyme Precursors / Q000494:pharmacology; D006632:Histamine / Q000032:analysis*; D006801:Humans; D009504:Neutrophils / Q000502:physiology*; D013050:Spectrometry, Fluorescence
D004792:Enzyme Precursors; D006632:Histamine; D006631:Amine Oxidase (Copper-Containing)