title: [Preparative representation of the living eggs of Schistosoma mansoni].
abstract: To maintain the hatchability of miracidia, pure worm eggs of S. mansoni were collected in great quantities from liver and spleen of experimentally infected mice. For this purpose, the tissue was digested with papain, hyaluronidase and trypsin. Worm eggs in the residue were separated from remaining host cells, tissue materials and dead eggs by using the sieving chamber (sieves 200 microns x 200 microns and 28 microns x 28 microns) and by density gradient centrifugation.
authors: Abteilung für Tropenmedizin und Infektionskrankheiten, Universität Rostock.
authors: W
authors: W
authors: Schlisio
authors: M
authors: M
authors: Lafrenz
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D006821:Hyaluronoglucosaminidase / Q000378:metabolism; D008099:Liver / Q000469:parasitology; D051379:Mice; D010063:Ovum / Q000502:physiology; D010206:Papain / Q000378:metabolism; D010274:Parasitology / Q000379:methods*; D012550:Schistosoma mansoni / Q000302:isolation & purification* / Q000502:physiology; D012555:Schistosomiasis mansoni / Q000469:parasitology*; D013154:Spleen / Q000469:parasitology; D014357:Trypsin / Q000378:metabolism
chemical_list: D006821:Hyaluronoglucosaminidase; D014357:Trypsin; D010206:Papain
vernacular_title: Präparative Darstellung lebender Eier von Schistosoma mansoni.