Defective plasma membrane H(+)-ATPase or orthovanadate resistant mutants from Candida albicans, a pathogenic yeast.
Orthovanadate-resistant mutants of diploid yeast Candida albicans were isolated by using two step mutational process. Such mutants had altered plasma membrane H(+)-ATPase activity. Based on the levels of PM-ATPase activity, these mutants could be grouped into two categories; one group included those mutants which did not exhibit reduction in PM-ATPase activity while the other displayed a reduction of upto 40% in enzyme activity. These mutants exhibited a number of distinct phenotypic characteristics and altered abilities with regard to phenotypic divergence. Results demonstrate the importance of PM-ATPase in overall physiology of this pathogenic yeast.
Mahanty
S K
SK
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Gupta
P
P
Banerjee
U
U
Fotedar
R
R
Prasad
R
R
D000818:Animals; D002176:Candida albicans / Q000201:enzymology* / Q000235:genetics; D002462:Cell Membrane / Q000201:enzymology; D004352:Drug Resistance, Microbial / Q000235:genetics; D006801:Humans; D009154:Mutation; D010641:Phenotype; D006180:Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*; D011817:Rabbits; D014638:Vanadates / Q000494:pharmacology*
D014638:Vanadates; D006180:Proton-Translocating ATPases