Regulation of eicosanoid synthesis by whole fetal rat brain ex vivo.
Intact cerebral hemispheres from 20-d-old rat fetuses incubated at 37 degrees C in Dulbecco's Modified Eagle Medium (DMEM) synthesize and release a number of arachidonic acid derived metabolites, such as thromboxane B2 (TxB2), 6-keto prostaglandin F1 alpha (6k-PGF1 alpha), and prostaglandin E2 (PGE2) eicosanoids. Synthesis is time-dependent and is stimulated upon addition of the calcium ionophore A23187 (10 microM). Ionophore stimulation is prevented by EDTA/EGTA (5 mM each) ion chelators, dextran-70 (5%), and indomethacin (10 microM), a potent cyclooxygenase inhibitor. Ca2+ (2 mM) enhances ionophore-mediated formation of TxB2, 6K-PGF1 alpha, and PGE2 by 2.5-, 2.9-, and 4.2-fold, respectively; Mg2+ blocks ionophore stimulation. Freezing and thawing enhances release of eicosanoids to a level nearly the same as that obtained in the presence of A23187, indicating a common mode of action.
Kunievsky
B
B
Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel.
Yavin
E
E
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000196:embryology / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D015925:Cryopreservation; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D015777:Eicosanoids / Q000096:biosynthesis*; D005615:Freezing; D005786:Gene Expression Regulation / Q000187:drug effects; D007213:Indomethacin / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D015777:Eicosanoids; D000001:Calcimycin; D004533:Egtazic Acid; D004492:Edetic Acid; D008274:Magnesium; D002118:Calcium; D007213:Indomethacin