title: Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H(+)-adenosine triphosphatase.
abstract: The TFP1 gene of the yeast Saccharomyces cerevisiae encodes two proteins: the 69-kilodalton (kD) catalytic subunit of the vacuolar proton-translocating adenosine triphosphatase (H(+)-ATPase) and a 50-kD protein. The 69-kD subunit is encoded by the 5' and 3' thirds of the TFP1 coding region, whereas the 50-kD protein is encoded by the central third. Evidence is presented that both the 69-kD and 50-kD proteins are obtained from a single translation product that is cleaved to release the 50-kD protein and spliced to form the 69-kD subunit.
authors: Institute of Molecular Biology, University of Oregon, Eugene 97403.
authors: P M
authors: PM
authors: Kane
authors: C T
authors: CT
authors: Yamashiro
authors: D F
authors: DF
authors: Wolczyk
authors: N
authors: N
authors: Neff
authors: M
authors: M
authors: Goebl
authors: T H
authors: TH
authors: Stevens
mesh_terms: D000818:Animals; D005800:Genes, Fungal; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D006180:Proton-Translocating ATPases / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D014617:Vacuoles / Q000201:enzymology*
chemical_list: D012333:RNA, Messenger; D006180:Proton-Translocating ATPases