title: Human CD8+ intraepithelial T lymphocytes are mainly CD45RA-RB+ and show increased co-expression of CD45R0 in celiac disease.
abstract: Expression of various CD45 isoforms (RA, RB and R0) on CD3+, CD4+ and CD8+ intraepithelial and lamina propria T cells was examined in situ by a three-color immunofluorescence technique in jejunal biopsy specimens from 32 patients with celiac disease and 18 controls. The median percentage of CD3+ intraepithelial lymphocytes (IEL) that expressed CD45R0 increased from 52% in controls to 69% in untreated celiac disease (p less than 0.01). Furthermore, the percentages of CD4+ and CD8+ IEL strongly positive for CD45R0 rose respectively from 94% and 24% in controls to 100% and 55% in untreated celiac disease. Conversely, CD45R0 was strongly expressed on most CD3+ lamina propria lymphocytes (LPL) both in control (81%) and diseased (77%-81%) mucosa. A variable fraction of the intraepithelial and lamina propria CD3+ T cells expressed mainly CD45RB (controls, 46% and 20%, respectively; celiac disease, 29% and 15%). Only 2% IEL and 4% LPL were positive for CD45RA. Expression of different CD45R isoforms thus identified three distinct CD8+ T cell subsets in human intestinal mucosa. In addition, our results suggested that antigen-primed CD8+CD45R0+ memory cells accumulate in the jejunal epithelium of patients with untreated celiac disease.
authors: Laboratory for Immunohistochemistry and Immunopathology (LIIPAT), University of Oslo, Norway.
authors: T S
authors: TS
authors: Halstensen
authors: H
authors: H
authors: Scott
authors: P
authors: P
authors: Brandtzaeg
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000943:Antigens, Differentiation / Q000096:biosynthesis*; D002446:Celiac Disease / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004848:Epithelium / Q000276:immunology; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D006649:Histocompatibility Antigens / Q000096:biosynthesis*; D006801:Humans; D007413:Intestinal Mucosa / Q000276:immunology; D007422:Intestines / Q000276:immunology; D007519:Isoantigens / Q000096:biosynthesis*; D017493:Leukocyte Common Antigens; D008875:Middle Aged; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000943:Antigens, Differentiation; D006649:Histocompatibility Antigens; D007519:Isoantigens; D017493:Leukocyte Common Antigens