title: Bovine lens aldose reductase: tight binding of the pyridine coenzyme.
abstract: Analysis by HPLC of the protein-free supernatant obtained after denaturation of aldose reductase shows that the native form of the enzyme (ARb) contains a tightly bound NADP+, which is absent in the oxidatively modified form (ARa). The absorption, fluorescence, and circular dichroism spectra of ARb and ARa are consistent with the presence of the cofactor only in the native form of aldose reductase. On the other hand, the modified enzyme, in appropriate thiol reducing conditions, can tightly bind NADP+. This indicates a potential reversibility of the modification of aldose reductase, at least in terms of retention of the cofactor.
authors: Dipartimento di Fisiologia e Biochimica, Universitá di Pisa, Italy.
authors: A
authors: A
authors: Del Corso
authors: D
authors: D
authors: Barsacchi
authors: M
authors: M
authors: Giannessi
authors: M G
authors: MG
authors: Tozzi
authors: M
authors: M
authors: Camici
authors: J L
authors: JL
authors: Houben
authors: M
authors: M
authors: Zandomeneghi
authors: U
authors: U
authors: Mura
mesh_terms: D000449:Aldehyde Reductase / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D004229:Dithiothreitol / Q000494:pharmacology; D007908:Lens, Crystalline / Q000201:enzymology*; D009249:NADP / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D011485:Protein Binding; D011487:Protein Conformation / Q000187:drug effects; D011489:Protein Denaturation; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D009249:NADP; D000449:Aldehyde Reductase; D004229:Dithiothreitol