Production of lipocortin-like proteins by cultured human tracheal submucosal gland cells.
Evidence is obtained for the presence of lipocortin-like proteins in human tracheal gland cells in culture. Using polyclonal antibodies to lipocortin I, indirect immunofluorescence studies demonstrate that lipocortin I is mainly confined to the tracheal gland cell surface. From cell membranes, four Ca2(+)-dependent proteins (35, 40, 45 and 67 kDa) were identified as lipocortin related proteins by using immunoblotting and fluorography following [35S]methionine metabolic labeling experiments. A strong immunoreactivity for the 35 kDa protein was observed. In addition, lipocortin-like proteins with apparent Mr33, 35, 37 and 67 kDa, respectively, were released in the apical culture medium by tracheal gland cells cultured on microporous membrane of a double chamber culture system.
Jacquot
J
J
INSERM U314, Université de Reims, CHU Maison Blanche, France.
Dupuit
F
F
Elbtaouri
H
H
Hinnrasky
J
J
Antonicelli
F
F
Haye
B
B
Puchelle
E
E
D017302:Annexins; D002135:Calcium-Binding Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000096:biosynthesis; D006801:Humans; D007700:Kinetics; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D009092:Mucous Membrane / Q000378:metabolism; D014132:Trachea / Q000378:metabolism*
D017302:Annexins; D002135:Calcium-Binding Proteins; D006023:Glycoproteins; D008715:Methionine