Interleukin-2 receptor is similarly expressed by activated lymphocytes from patients on chronic hemodialysis and healthy subjects.
We studied peripheral blood mononuclear cells (PBMC) from 10 patients on chronic hemodialysis vs 10 healthy controls. T cells were counted by E rosette formation. Interleukin (IL)-2 receptor (IL-2R) carrier cells were identified by Dako-IL-2R, which was chosen as a monoclonal antibody that binds to the alpha chain of IL-2R without interrupting the cell machinery of mitogen-induced IL production. Fluorescein-tagged goat anti-rabbit IgG1* was used for subsequent cell staining, and cells were counted by FACS. Net numbers of PHA-activated IL-2R carrier cells were derived by subtracting appropriate background controls. The average number of these cells relative to numbers of total T cell population in uremic PBMC was statistically not different from healthy controls. Several defects in cellular activation associated with IL-2 mechanisms could be envisaged to account for the impaired proliferative response to PHA, manifested by uremic T cells.
Modai
D
D
Nephrology Division, Assaf Harofeh Medical Center, Zerifin, Israel.
Berman
S
S
Sheleg
Y
Y
Cohn
M
M
Weissgarten
J
J
Averbukh
Z
Z
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000648:ultrastructure*; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D015375:Receptors, Interleukin-2 / Q000502:physiology*; D006435:Renal Dialysis; D012397:Rosette Formation; D050378:T-Lymphocytes, Regulatory / Q000166:cytology; D014511:Uremia / Q000097:blood*
D015375:Receptors, Interleukin-2