One-step fractionation method for isolating H1 histones from chromatin under nondenaturing conditions.
By combining conventional methods of chromatin preparation with ion-exchange chromatography in CM-Sephadex C-25, we have been able to isolate and fractionate histone H1 variants from different sources. This method of fractionation is very simple and allows one to obtain, very rapidly, large amounts of these histones in a native nondenatured conformation.
Garcia-Ramirez
M
M
Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331-6503.
Leuba
S H
SH
Ausio
J
J
D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000737:chemistry / Q000302:isolation & purification*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000737:chemistry; D006657:Histones / Q000737:chemistry / Q000302:isolation & purification*; D011487:Protein Conformation; D014407:Tumor Cells, Cultured / Q000737:chemistry
D002843:Chromatin; D006657:Histones