[Immunological studies of the activity of Aspergillus flavus Link. I. Antigen analysis and evaluation of its chemical composition and toxicity].
The aim of this study was to get insight into chemical structure and toxigenicity of antigenic preparations obtained from A. flavus Link strain isolated from industrial environment. A microbiol multiplication and an antigenic fractions preparation scheme is presented. The method proposed allows to obtain antigens from culture filtrate (APP), mycelial extract (AEM), and two subfractions obtained from AEM: supernatant--AS and precipitated--API. The strain tested did not show aflatoxigenic activity and antigenic fractions obtained were free of aflatoxin B1, B2, G1, G2 in concentrations detectable by thin-layer chromatography. A chemical composition of the antigenic fractions was tested. A content, depending on fractions, of proteins ranged from 32.0 to 74.5 micrograms/ml, of sugars from 15.0-44.5 micrograms/ml, phosphorus 0.5-1.5 micrograms/ml, and nitrogen 2.5-4.9 micrograms/ml. Toxicity of APP and AEM antigens designated for laboratory animals immunisation was also determined. The values of LD50 for APP preparation was 2.00 mg/mouse and for AEM - 2.75 mg/mouse. These data give evidence of moderate toxicity of these preparations.
Biś
W A
WA
Zakład Mikrobiologii Uniwersytefu im. A. Mickiewicza w Poznaniu.
D000818:Animals; D000946:Antigens, Fungal / Q000032:analysis* / Q000633:toxicity; D001231:Aspergillus flavus / Q000276:immunology*; D002241:Carbohydrates / Q000032:analysis; D003470:Culture Media; D005656:Fungal Proteins / Q000032:analysis; D066298:In Vitro Techniques; D051379:Mice; D010758:Phosphorus / Q000032:analysis; D011044:Poland; D014865:Waste Disposal, Fluid; D014871:Water Microbiology
D000946:Antigens, Fungal; D002241:Carbohydrates; D003470:Culture Media; D005656:Fungal Proteins; D010758:Phosphorus
Badania immunologicznej aktywności Aspergillus flavus link. I. Otrzymywanie antygenów, ocena składu chemicznego i ich toksyczności.