title: Amplification and analysis of human DNA present in mosquito bloodmeals.
abstract: DNA fingerprinting should permit the identification of individual human hosts of haematophagous arthropods, providing epidemiologically useful information, for example, the biting rates on different people and the impact of insecticide-impregnated bednets. Investigations reported here demonstrate that it is possible to extract, amplify and fingerprint human DNA from the bloodmeals of individual female Anopheles gambiae mosquitoes kept at 24 degrees C for up to 10-15 h post-ingestion.
authors: Department of Biochemistry, Imperial College of Science, Technology and Medicine, London.
authors: R M
authors: RM
authors: Coulson
authors: C F
authors: CF
authors: Curtis
authors: P D
authors: PD
authors: Ready
authors: N
authors: N
authors: Hill
authors: D F
authors: DF
authors: Smith
mesh_terms: D000818:Animals; D000852:Anopheles / Q000502:physiology*; D004247:DNA / Q000097:blood*; D016172:DNA Fingerprinting; D005247:Feeding Behavior; D005260:Female; D006801:Humans; D007299:Insect Bites and Stings / Q000453:epidemiology*; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results
chemical_list: D004247:DNA