Production of granulocyte-macrophage colony-stimulating factor by adult murine parenchymal liver cells (hepatocytes).
In order to investigate the hematolymphoid system in the adult murine liver, we examined colony-stimulating factor (CSF) released from primary cultured liver cell fractions, parenchymal and nonparenchymal liver cells (more than 99% pure. Using a fibrin clot culture system, we found colony-stimulating activity not only in the culture supernatant from nonparenchymal fraction of normal liver cells (NP-NLC sup), but also in the culture supernatant from parenchymal fraction of normal liver cells (P-NLC sup). Clusters and colonies, which were generated in 4-day cultures of bone marrow cells or spleen cells with P-NLC sup or NP-NLC sup, were mainly composed of granulocytes (G), macrophages (M), and granulocytes and macrophages (GM). Both P-NLC sup and NP-NLC sup proliferated IC2, which is a GM-CSF and IL-3 dependent cell line, and this proliferation was completely inhibited by rabbit anti-murine GM-CSF antibody. Furthermore, both Northern blot analysis and in situ hybridization with specific cDNA probe for murine GM-CSF showed that parenchymal liver cells expressed GM-CSF mRNA transcripts. The present findings indicate that cultured murine parenchymal liver cells produce GM-CSF. The possibility, therefore, is suggested that parenchymal liver cells also play an important role in the regional hematolymphoid system of the liver.
Sakamoto
T
T
Department of Microbiology and Immunology, Nippon Medical School, Tokyo, Japan.
Mabuchi
A
A
Kuriya
S
S
Sudo
T
T
Aida
T
T
Asano
G
G
Shoji
T
T
Yokomuro
K
K
D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D015342:DNA Probes; D005260:Female; D015870:Gene Expression; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D006098:Granulocytes / Q000276:immunology; D008099:Liver / Q000166:cytology / Q000276:immunology*; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000276:immunology
D015342:DNA Probes; D012333:RNA, Messenger; D016178:Granulocyte-Macrophage Colony-Stimulating Factor