A continuous fluorimetric assay for ATPase activity.
A new continuous coupled fluorimetric assay is described for ATPases in general. Thus phosphate released from ATP hydrolysis is coupled to the nucleoside phosphorylase reaction using 7-methylguanosine as a fluorescent substrate for the nucleoside phosphorylase reaction. The hydrolysis of 7-methylguanosine leads to 7-methylguanine, which has lower quantum yield and hence can be used to monitor ATP hydrolysis continuously. The method has the potential to be extended to GTPase and nucleotidyltransferase assays.
Banik
U
U
Department of Biophysics, Bose Institute, Calcutta, India.
Roy
S
S
D000818:Animals; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D009218:Myosins / Q000032:analysis*; D009994:Osmolar Concentration; D010710:Phosphates / Q000378:metabolism; D011817:Rabbits; D013050:Spectrometry, Fluorescence
D010710:Phosphates; D009218:Myosins