Effect of the EBNA-2 gene on the surface antigen phenotype of transfected EBV-negative B-lymphoma lines.
Two EBV-negative B-lymphoma cell lines with different phenotypes were transfected with the Epstein-Barr virus EBNA-2 gene. The effects on the expression of 8 B-cell surface markers were analyzed by immunofluorescence methods. In one of the EBNA-2 transfected cell lines, the expression of the CR2 receptor CD21 was induced and the expression of CD23 was enhanced. The results suggest that the EBNA-2 gene is involved in the regulation of CD21 and CD23 in EBV-carrying cells.
Aman
P
P
Dept. of Tumor Biology, Karolinska Institute, Stockholm, Sweden.
Rowe
M
M
Kai
C
C
Finke
J
J
Rymo
L
L
Klein
E
E
Klein
G
G
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000032:analysis; D000951:Antigens, Neoplasm / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics; D001402:B-Lymphocytes; D002460:Cell Line; D003360:Cosmids / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D015967:Gene Expression Regulation, Viral / Q000235:genetics; D005814:Genes, Viral; D005822:Genetic Vectors / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D015151:Immunoblotting; D008223:Lymphoma / Q000235:genetics* / Q000276:immunology; D010641:Phenotype; D014162:Transfection / Q000235:genetics*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000951:Antigens, Neoplasm; D000954:Antigens, Surface