Discrete domains of human U6 snRNA required for the assembly of U4/U6 snRNP and splicing complexes.
U6 snRNA sequences required for assembly of U4/U6 snRNP and splicing complexes were determined by in vitro reconstitution of snRNPs. Both mutagenesis and chemical modification/interference assays identify a U6 snRNA domain required for U4/U6 snRNP formation. The results support the existence of a U4/U6 snRNA interaction domain previously proposed on the basis of phylogenetic evidence. In addition, two short U6 snRNA regions flanking the U4/U6 interaction domain are essential to assemble the U4/U6 snRNP into splicing complexes. These two regions may represent binding sites for splicing factors or may facilitate the formation of an alternative U6 snRNA secondary structure during spliceosome assembly.
Bindereif
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Max-Planck-Institut für Molekulare Genetik, Otto-Warburg-Laboratorium, Berlin, FRG.
Wolff
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Green
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MR
D001483:Base Sequence; D001665:Binding Sites; D055598:Chemical Phenomena; D002621:Chemistry; D007163:Immunosorbent Techniques; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009154:Mutation; D009690:Nucleic Acid Conformation; D012326:RNA Splicing / Q000235:genetics*; D012342:RNA, Small Nuclear / Q000235:genetics*; D012261:Ribonucleoproteins / Q000235:genetics*; D017411:Ribonucleoproteins, Small Nuclear; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic
D012342:RNA, Small Nuclear; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear