Identification and characterization of a Neisseria gonorrhoeae gene encoding a glycolipid-binding adhesin.
We recently identified a set of mammalian cell receptors for Neisseria gonorrhoeae that are glycolipids. These receptors, lactosylceramide [Gal(beta 1-4)Glc(beta 1-1)Cer], gangliotriosylceramide [GalNAc( beta 1-4)Gal(beta 1-4)Glc(beta 1-1)Cer], and gangliotetraosylceramide [Gal(beta 1-3)GalNAc(beta 1-4)Gal(beta 1-4)Glc(beta 1-1)Cer], were shown to be specifically bound by a gonococcal outer membrane protein distinct from pilin and protein II. Here we report the isolation of the gene encoding the gangliotetraosylceramide-binding adhesin from a N. gonorrhoeae MS11 gene bank in Escherichia coli. Transposon mutagenesis studies in E. coli indicate that the adhesion is a protein with a molecular mass of 36,000 Da. The gene encoding the 36-kDa protein is duplicated in MS11 since two transposon insertions were required to abolish expression of the gene in this bacterium. This protein is present on the surface of the gonococcus and is not associated with the pilus.
Paruchuri
D K
DK
Department of Molecular Biology, Scripps Clinic and Research Foundation, La Jolla, CA 92037.
Seifert
H S
HS
Ajioka
R S
RS
Karlsson
K A
KA
So
M
M
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000302:isolation & purification; D015139:Blotting, Southern; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular / Q000379:methods; D004251:DNA Transposable Elements; D005798:Genes, Bacterial; D015698:Genomic Library; D006017:Glycolipids / Q000378:metabolism*; D006028:Glycosphingolipids / Q000378:metabolism; D007106:Immune Sera; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009154:Mutation; D009344:Neisseria gonorrhoeae / Q000235:genetics* / Q000502:physiology / Q000648:ultrastructure; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011485:Protein Binding
D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D004251:DNA Transposable Elements; D006017:Glycolipids; D006028:Glycosphingolipids; D007106:Immune Sera