Cloning and characterization of a DNA fragment that confers sulfonamide resistance in a serogroup B, serotype 15 strain of Neisseria meningitidis.
By cloning studies and complementation experiments, the sulfonamide resistance gene of a serogroup B and serotype 15 (B:15) strain of Neisseria meningitidis was localized to a 1.2-kb chromosomal SspI fragment expressing a drug-resistant dihydropteroate synthase. The fragment hybridized to DNA from both resistant and susceptible strains, suggesting that the resistance gene is a variant of the normal gene for dihydropteroate synthase.
Kristiansen
B E
BE
Telelab A/S, Skien, Norway.
Rådström
P
P
Jenkins
A
A
Ask
E
E
Facinelli
B
B
Sköld
O
O
D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis*; D015247:Deoxyribonuclease HindIII; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial; D009345:Neisseria meningitidis / Q000187:drug effects* / Q000235:genetics; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D013449:Sulfonamides / Q000494:pharmacology*; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D013449:Sulfonamides; D015247:Deoxyribonuclease HindIII