title: The molecular structure of the Corynebacterium glutamicum threonine synthase gene.
abstract: The minimal region encoding the Corynebacterium glutamicum threonine synthase structural gene and its promoter was mapped by deletion analysis and complementation of the C. glutamicum thrC allele to a 1.6 kb region of the recombinant plasmid pFS80. The nucleotide sequence of this and flanking DNA was determined. The transcription and translation start points were identified by S1 mapping analysis and amino-terminal protein sequencing, respectively. The thrC gene encodes a 54481-Dalton polypeptide product. Translation of the thrC mRNA initiates only six nucleotides downstream from transcription. The length of the mRNA transcript is consistent with a single gene transcription unit. The C. glutamicum thrC gene is expressed independently of the other threonine-specific genes hom and thrB.
authors: Seoul Miwon Co. Ltd, Dobong-ku, Korea.
authors: K S
authors: KS
authors: Han
authors: J A
authors: JA
authors: Archer
authors: A J
authors: AJ
authors: Sinskey
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology; D015152:Blotting, Northern; D019757:Carbon-Oxygen Lyases; D003352:Corynebacterium / Q000201:enzymology* / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology; D005798:Genes, Bacterial; D008190:Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010957:Plasmids; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D008190:Lyases; D019757:Carbon-Oxygen Lyases; C023160:threonine synthase