Uptake and photodynamic efficiency of hematoporphyrin, hydroxyethylvinyldeuteroporphyrin and hematoporphyrin derivative (Photofrin II): a study with isolated mitochondria.
The uptake of Photofrin II (PFII), hematoporphyrin (Hp) and hydroxyethylvinyldeuteroporphyrin (HVD) by isolated mitochondria was studied using the high performance liquid chromatography (HPLC) technique. The various PFII components show a high affinity for mitochondria. At 5.75 micrograms/ml PFII, their ratio of incorporation was found to be very similar, except for Hp which is about two times less incorporated. These results were reproduced with pure Hp and pure HVD. The uptake of Hp and HVD increases with concentration but, while that of Hp reaches a plateau, the uptake of HVD continues to increase. At a high porphyrin concentration (approximately 10(-5) M), the loss of respiratory control is obtained with the same light dose for Hp and PFII. Taking into account the uptake and the known photophysical parameters of the various porphyrins, the photodynamic efficiency of HVD seems equivalent to that of Hp. The present results and known data on cell photoinactivation suggest that the activity of these porphyrins is mainly dependent on their incorporation.
Dellinger
M
M
Laboratoire de Biophysique, INSERM U.201, CNRS UA.481, Muséum National d'Histoire Naturelle, Paris, France.
Vever-Bizet
C
C
Brault
D
D
Moreno
G
G
Salet
C
C
D000818:Animals; D001692:Biological Transport; D003905:Deuteroporphyrins / Q000378:metabolism* / Q000494:pharmacology; D017323:Dihematoporphyrin Ether; D006415:Hematoporphyrins / Q000378:metabolism* / Q000494:pharmacology*; D007700:Kinetics; D008027:Light; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D011166:Porphyrins / Q000378:metabolism*; D011838:Radiation-Sensitizing Agents / Q000378:metabolism*; D051381:Rats
D003905:Deuteroporphyrins; D006415:Hematoporphyrins; D011166:Porphyrins; D011838:Radiation-Sensitizing Agents; C049889:hydroxyethylvinyldeuteroporphyrin; D017323:Dihematoporphyrin Ether