Effect of soluble and immobilized plasmin on fibrinogen and platelets.
Plasmin was immobilized on collageno-elastic tubes (CET) using carbodiimide as the cross-linking agent. The effects of plasmin-CET grafts and corresponding soluble plasmin on fibrinogen, thrombin-mediated fibrinogen activation, and platelet activity, were investigated. There was a significant increase in fibrinogen deposition on plasmin-CETs over non-plasmin (i.e. control) CETs. Furthermore, exposure of fibrinogen to plasmin CETs enhances its deposition to control grafts situated downstream. Plasmin-bound CETs retained higher platelet deposition when preliminarily coated with fibrinogen. Finally, plasmin exerted a positive effect on thrombin-mediated fibrinogen activation at low plasmin concentrations. A mechanistic hypothesis aimed at interpreting this finding is proposed.
Senatore
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Department of Chemical Engineering, Texas Tech University, Lubbock 79409.
Shankar
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Ho
C H
CH
Avantsa
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S
Allon
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Posteraro
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R
D000818:Animals; D001792:Blood Platelets / Q000187:drug effects*; D003094:Collagen; D004800:Enzymes, Immobilized / Q000494:pharmacology*; D005340:Fibrinogen / Q000187:drug effects*; D005341:Fibrinolysin / Q000494:pharmacology*; D011817:Rabbits; D012995:Solubility
D004800:Enzymes, Immobilized; D005340:Fibrinogen; D003094:Collagen; D005341:Fibrinolysin