title: The HL-60 model for the interaction of human macrophages with the Legionnaires' disease bacterium.
abstract: The facultative intracellular pathogen, Legionella pneumophila, multiplies within and kills human monocytes and alveolar macrophages. We show that L. pneumophila strain Philadelphia-1 infects, multiplies within and kills the promyelocyte HL-60 cell line after its differentiation into macrophage-like cells. The characteristics of the interaction between L. pneumophila and differentiated HL-60 cells closely resemble those between L. pneumophila and human peripheral blood monocytes. With both cell types, C receptors and serum C mediate attachment of L. pneumophila, which are taken up by coiling phagocytosis. The replicative phagosome is lined with ribosomes; intracellular multiplication is iron-dependent; and replicating bacteria ultimately destroy the host cell. As in human monocytes, an avirulent mutant derivative of L. pneumophila Philadelphia-1, 25D, does not replicate in and is not cytopathic for differentiated HL-60 cells. Differentiated HL-60 cells therefore provide a convenient and faithful model for the study of L. pneumophila-mononuclear phagocyte interaction.
authors: Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
authors: A
authors: A
authors: Marra
authors: M A
authors: MA
authors: Horwitz
authors: H A
authors: HA
authors: Shuman
mesh_terms: D001422:Bacterial Adhesion; D002448:Cell Adhesion; D003176:Complement C3 / Q000378:metabolism; D003165:Complement System Proteins / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D007501:Iron / Q000502:physiology; D007875:Legionella / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity; D008264:Macrophages / Q000276:immunology / Q000382:microbiology*; D010587:Phagocytosis; D010588:Phagosomes / Q000648:ultrastructure; D011951:Receptors, Complement / Q000502:physiology; D017463:Receptors, Complement 3b; D012270:Ribosomes / Q000648:ultrastructure; D014407:Tumor Cells, Cultured
chemical_list: D003176:Complement C3; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D003165:Complement System Proteins; D007501:Iron