Impact of 90Sr on mouse natural killer cells and their regulation by alpha interferon and interleukin 2.
Male CBA/SU mice were exposed to ionizing radiation by intraperitoneal injection of the bone-seeking beta-emitter 90Sr. NK-cell lytic activities in spleen, peripheral blood, and lymph nodes were severely depressed or completely abolished. In contrast, production of the NK regulatory proteins alpha interferon (IFN-alpha) and interleukin 2 (IL-2) was normal 5-8 weeks after 90Sr injection. IFN-alpha, produced in vivo or in vitro by cells from injected mice, was able to enhance strongly NK lytic activities. These data indicate that 90Sr acts on the bone marrow, where it interferes with the maturation and seeding of NK precursor cells. The mechanisms regulating NK activities in peripheral organs remained relatively unchanged. Finally, we did not detect any major organ redistribution of NK cells as a result of 90Sr irradiation.
Gidlund
M
M
Department of Immunology, Karolinska Institute, Stockholm, Sweden.
Bierke
P
P
Orn
A
A
Axberg
I
I
Ramstedt
U
U
Wigzell
H
H
D000818:Animals; D007274:Injections, Intraperitoneal; D007370:Interferon Type I / Q000096:biosynthesis / Q000502:physiology*; D007376:Interleukin-2 / Q000096:biosynthesis / Q000502:physiology*; D007694:Killer Cells, Natural / Q000502:physiology / Q000528:radiation effects*; D008198:Lymph Nodes / Q000166:cytology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D011070:Poly I-C / Q000494:pharmacology; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000166:cytology; D013326:Strontium Radioisotopes / Q000008:administration & dosage; D013994:Tilorone / Q000494:pharmacology
D007370:Interferon Type I; D007376:Interleukin-2; D013326:Strontium Radioisotopes; D013994:Tilorone; D011070:Poly I-C