title: Proliferation and differentiation of a transfected Schwann cell line is altered by an artificial basement membrane.
abstract: Rapidly dividing transfected Schwann cells were grown on Matrigel, a reconstituted basement membrane gel. Matrigel decreased the proliferation of the cells by 75% when compared to sister cultures that were grown on an untreated plastic substrate. Some transfected cells plated onto a Matrigel substrate formed colonies similar to that observed when the cells were plated on a plastic substrate. Additionally, many cells on Matrigel assembled themselves into fascicles projecting away from the colonies. These fascicles were composed of transfected Schwann cells that had assumed a bipolar appearance reminiscent of quiescent secondary Schwann cells in culture. Transfected cells grown on Matrigel contained approximately 10-fold less glial fibrillary acidic protein when compared to sister cultures grown on an untreated plastic substrate. By indirect immunofluorescence laminin immunoreactivity appeared as globules within the cytoplasm of the cells which were cultured on a plastic substrate. However, cells that were grown on the Matrigel substrate appear to organize laminin in a linear array around themselves. These results demonstrate that the presence of an artificial basement membrane alters the morphology, rate of proliferation, and state of differentiation of a transfected Schwann cell line.
authors: Department of Psychology, Colgate University, Hamilton, New York 13346.
authors: J E
authors: JE
authors: Yoshino
authors: T J
authors: TJ
authors: Neuberger
authors: C J
authors: CJ
authors: Cornbrooks
authors: G I
authors: GI
authors: Tennekoon
authors: L F
authors: LF
authors: Eng
authors: G H
authors: GH
authors: DeVries
mesh_terms: D001485:Basement Membrane; D001672:Biocompatible Materials; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003094:Collagen / Q000494:pharmacology*; D004338:Drug Combinations; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007797:Laminin / Q000378:metabolism / Q000494:pharmacology*; D008567:Membranes, Artificial; D011509:Proteoglycans / Q000494:pharmacology*; D012583:Schwann Cells / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D014018:Tissue Distribution; D014162:Transfection
chemical_list: D001672:Biocompatible Materials; D004338:Drug Combinations; D005904:Glial Fibrillary Acidic Protein; D007797:Laminin; D008567:Membranes, Artificial; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen