Human urinary erythropoietin: preparation with high potency.
Human urinary erythropoietin has been purified to homogeneity. The seven-step procedure yielded a preparation with a potency of 225,000 U/mg protein. SDS-polyacrylamide gel electrophoretic analysis of the purified hormone revealed a single protein band with a molecular weight of about 35,000 that migrated with the biological activity. As to its stability, the purified hormone retained its activity in the presence of 0.001% Tween 20.
Matsushita
J
J
Second Department of Internal Medicine, Kumamoto University Medical School, Japan.
Kawakita
M
M
Shibuya
K
K
Koishihara
Y
Y
Sakaguchi
M
M
Takatsuki
A
A
D000741:Anemia, Aplastic / Q000652:urine; D000818:Animals; D001681:Biological Assay / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D004591:Electrophoresis, Polyacrylamide Gel; D004921:Erythropoietin / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010198:Pancytopenia / Q000652:urine; D051381:Rats; D011919:Rats, Inbred Strains
D004921:Erythropoietin