Purine salvage enzyme activities in normal and neoplastic human tissues.
The enzymatic pattern of five enzymes involved in the purine salvage pathway, namely purine nucleoside phosphorylase (EC 2.4.2.1), adenosine deaminase (EC 3.5.4.4), 5'-nucleotidase (EC 3.1.3.5), alkaline phosphatase (EC 3.1.3.1), and hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) has been evaluated both in human intestinal and breast carcinomas and compared to that of normal tissues. A higher level of hypoxanthine-guanine phosphoribosyltransferase was associated with tumor tissues. This metabolic alteration should lead to an elevated synthesis of nucleotides in cancer cells, might confer selective growth advantages to neoplastic tissues, and account, at least in part, for the difficulties encountered in the chemotherapy of human tumors, by using compounds affecting only the purine de novo biosynthesis.
Camici
M
M
Istituto di Produzione Animale, Università di Udine, Pagnacco, Italy.
Tozzi
M G
MG
Allegrini
S
S
Del Corso
A
A
Sanfilippo
O
O
Daidone
M G
MG
De Marco
C
C
Ipata
P L
PL
D015720:5'-Nucleotidase / Q000032:analysis*; D000243:Adenosine Deaminase / Q000032:analysis*; D000469:Alkaline Phosphatase / Q000032:analysis*; D001943:Breast Neoplasms / Q000201:enzymology; D003110:Colonic Neoplasms / Q000201:enzymology; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000032:analysis*; D009363:Neoplasm Proteins / Q000032:analysis*; D011683:Purine-Nucleoside Phosphorylase / Q000032:analysis*; D011687:Purines / Q000378:metabolism; D012004:Rectal Neoplasms / Q000201:enzymology
D009363:Neoplasm Proteins; D011687:Purines; D011683:Purine-Nucleoside Phosphorylase; D007041:Hypoxanthine Phosphoribosyltransferase; D000469:Alkaline Phosphatase; D015720:5'-Nucleotidase; D000243:Adenosine Deaminase