S-phase detailed analysis in KB cells by bromdeoxyuridine labelling.
A method based on the sequential BrdU incorporation followed by differential spiralization of the chromosomes was used for analysis of S-phase in KB cells when Brdu was added to the cultures for 19, 24 and 26 hours before fixation. The S-phase was subdivided into three subphases, each recognisable by a specific incorporation pattern at metaphase: late-S (LS), middle-S (MS) and early -S (ES). Two other intermediary subphases, mid-early S (mES) and mid-late S (mLS) have also been distinguished but their accurate identification is generally difficult. A high degree of asynchrony in KB cells population was observed as well as a wide variability concerning the duration of S-phase (BrdU = bromdeoxyuridine).
Georgian
L
L
Victor Babeş Institute, Bucharest, Romania.
Ghyka
G
G
Savi
I
I
D001973:Bromodeoxyuridine / Q000378:metabolism*; D002460:Cell Line; D002871:Chromosome Banding; D002875:Chromosomes / Q000648:ultrastructure; D004247:DNA / Q000096:biosynthesis*; D016196:S Phase
D004247:DNA; D001973:Bromodeoxyuridine