[Screening of beta-N-acetylhexosaminidase forming strains and conditions for enzyme production].
About 1200 strains of microorganisms were screened including fungi, actinomyces, and bacteria, in which 237 strains producing the enzyme desired. The results showed that the beta-GlcNAcase and beta-GalNAcase always co-existed in one strain, though may be in different ratio. From strains mentioned above the authors screened out a potent beta-N-acetylhexosaminidase producing strain, Aspergillus tamarii S215, from the soil sample. The optimal conditions for enzyme production were as follows: the microorganisms was inoculated in a 5% wheat bran suspension, cultured at 28-30 degrees C on shaker for 5-6 days. The productivity can be moderately enhanced by the addition of cellobiose or glucosamine or galactosamine or by the extra supplement of (NH4)2SO4 and NH4NO3 as N sources. In the culture filtrate of Asp. tamarii, the alpha, (beta)-galactosidase, beta-glucosidase, alpha-mannosidase and beta-fucosidase were also found.
Yan
Z Z
ZZ
Institute of Microbiology, Academia Sinica, Beijing.
Tao
Y
Y
Cheng
X L
XL
Sun
J W
JW
Zhang
S Z
SZ
D001230:Aspergillus / Q000302:isolation & purification* / Q000378:metabolism; D012987:Soil / Q000032:analysis; D001619:beta-N-Acetylhexosaminidases / Q000096:biosynthesis*
D012987:Soil; D001619:beta-N-Acetylhexosaminidases