Continuous monitoring of blood-brain barrier opening to Cr51-EDTA by microdialysis following probe injury.
Continuous detection of the permeability changes of the blood-brain (BB) interface became feasible with the recent development of the cerebral microdialysis technique. Vasogenic brain oedema was induced by the insertion of the dialysis capillary into the cerebral cortex of rats, and blood-brain transfer was measured with Cr51-EDTA as a blood-born tracer. The dialysis capillaries had an upper limit of 500 daltons (MW). Ringer solution was perfused through the dialysis tube. Samples were collected continuously. The permeability coefficient has been calculated from the radioactivity of the serum and of the washing fluid.
Major
O
O
National Institute of Neurosurgery, Budapest.
Shdanova
T
T
Duffek
L
L
Nagy
Z
Z
D000818:Animals; D001812:Blood-Brain Barrier; D002860:Chromium Radioisotopes; D003956:Dialysis / Q000379:methods; D004492:Edetic Acid / Q000378:metabolism / Q000493:pharmacokinetics*; D008297:Male; D008959:Models, Neurological; D008991:Monitoring, Physiologic; D051381:Rats; D011919:Rats, Inbred Strains
D002860:Chromium Radioisotopes; D004492:Edetic Acid