title: Sequence analysis of the promoter region of the rat vasopressin gene.
abstract: The vasopressin gene is highly transcribed in magnocellular neurons of the supraoptic (SON) and paraventricular nucleus (PVN) in the rat hypothalamus. In order to identify cis-acting elements involved in the expression of the vasopressin gene, approximately 1 kb upstream of the transcription start site has been sequenced. Several putative regulatory elements have been detected, including a glucocorticoid response element (CRE), a cAMP response element (CRE), and four AP2 binding sites. In gel shift assays performed with a labelled DNA fragment corresponding to nucleotide residues -214 to -36 and nuclear proteins extracted from SON-derived tissue enriched in magnocellular neurons, three specific protein-DNA complexes have been detected. Complex formation is effectively competed by addition of an excess of unlabelled fragment.
authors: Institut für Zellbiochemie und Klinische Neurobiologie, Universitäts-Krankenhaus Eppendorf, Hamburg, FRG.
authors: E
authors: E
authors: Mohr
authors: D
authors: D
authors: Richter
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D000242:Cyclic AMP / Q000502:physiology; D004247:DNA / Q000032:analysis; D004268:DNA-Binding Proteins / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005809:Genes, Regulator / Q000502:physiology; D005938:Glucocorticoids / Q000502:physiology; D006868:Hydrolysis; D007031:Hypothalamus / Q000032:analysis; D008969:Molecular Sequence Data; D009474:Neurons / Q000032:analysis; D009687:Nuclear Proteins / Q000032:analysis; D010446:Peptide Fragments / Q000032:analysis; D011401:Promoter Regions, Genetic; D051381:Rats; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014667:Vasopressins / Q000032:analysis / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D005938:Glucocorticoids; D009687:Nuclear Proteins; D010446:Peptide Fragments; D014667:Vasopressins; D004247:DNA; D000242:Cyclic AMP