Neuro-humoral control of insulin secretion.
Hypothalamic extract from normal rats was shown to contain a principle able to stimulate insulin secretion both in vivo and in vitro. This substance, whose peptidic nature was demonstrated by enzymatic digestion, has a molecular weight of about 1000 Daltons. An active release of this peptide was obtained when incubating hypothalamic fragments under appropriate stimulatory conditions (i.e. KCl 50 mmol l-1 in the incubation medium) (i.e. molecular weight and insulin secretion stimulating activity) similar to the hypothalamic peptide was evidentiated in the plasma of normal rats. Since manipulations of hte hypothalamus (i.e. electrical lesions or stimulations) could respectively decrease or enhance the plasma concentration of this substance, the hypothalamic origin of the insulin secretion stimulating principle present in the plasma was strongly suggested. The possible participation of this hypothalamic peptide to the neural control of insulin secretion is suggested.
Bobbioni-Harsch
E
E
Laboratoires de Recherches Métaboliques, Faculty of Medicine, University of Geneva, Switzerland.
Jeanrenaud
B
B
D000818:Animals; D007028:Hypothalamic Hormones / Q000302:isolation & purification / Q000494:pharmacology / Q000502:physiology; D007031:Hypothalamus / Q000502:physiology*; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism; D009479:Neuropeptides / Q000302:isolation & purification / Q000494:pharmacology / Q000502:physiology; D051381:Rats
D007028:Hypothalamic Hormones; D007328:Insulin; D009479:Neuropeptides