Detection of provirus in an HTLV-II producer CD8+ T cell line by polymerase chain reaction combined with digoxigenin-ELISA method.
A human T-cell leukemia virus type II (HTLV-II) producer cell line, designated HTLV-IIA, was established by cocultivation with leukocytes from an anti-human T-cell leukemia type I (HTLV-I) antibody-positive white male intravenous drug abuser and a healthy Japanese female. The cell line was examined for viral antigens by the indirect immunofluorescence method. The cytoplasm of over 80% of the cells was brilliantly stained. Cytogenetically, the cell line has a normal female karyotype. Electron microscopy of the HTLV-IIA disclosed many C-type retrovirus particles of mature, immature and non-cored types in the extracellular spaces. The surface markers of the transformed cells are CD2+, CD3+, CD4- and CD8+. To distinguish between HTLV-I and HTLV-II infection in the cell line, a method for detection of the HTLV-II provirus was developed by combining the polymerase chain reaction method with digoxigenin-enzyme-linked immunosorbent assay method.
Miyamoto
K
K
School of Health Sciences, Okayama University.
Tomita
N
N
Ohtsuki
Y
Y
Kitajima
K
K
D000328:Adult; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D016827:CD8 Antigens; D002460:Cell Line / Q000276:immunology; D002461:Cell Line, Transformed; D004076:Digoxigenin; D004077:Digoxin / Q000031:analogs & derivatives*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D015698:Genomic Library; D015491:HTLV-II Infections / Q000175:diagnosis*; D015367:Human T-lymphotropic virus 2 / Q000235:genetics*; D006801:Humans; D008214:Lymphocytes / Q000502:physiology; D008297:Male; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics*; D050378:T-Lymphocytes, Regulatory / Q000502:physiology
D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens; D004077:Digoxin; D004076:Digoxigenin