IgE receptors (FcR epsilon) modifications. I. Effect of regulator soluble factors of human IgE synthesis isolated by affinity chromatography.
After isolating the soluble factors and observing their biological activity of "in vitro" IgE synthesis, we decided to study the changes these molecules exercise on the expression of lymphocytes of IgE (FcR epsilon). We saw that the fractions from healthy subjects inhibited the expression of receptors quantified by immunofluorescence, reducing it to 15.5 and 15.75 (p less than 0.01). Three of the fractions from atopic individuals also inhibited this expression, with some average values of 13.75, 11.25 and 15.25, against 23 as the basal levels. Material from pollinic patients acted similarly, obtaining expression percentages of 13 and 14.5 (p less than 0.01). These findings, along with those obtained in the experiment of IgE synthesis, confirmed the presence of regulator factors in IgE synthesis in the 3 population groups, quantitatively different. These factors will be isotype specific and just as what happened in IgG synthesis, there was no modification in the expression of the mentioned immunoglobulin.
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Departamento de Alergología, Facultad de Medicina, Universidad de Navarra, Pamplona, Spain.
Sanz
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ML
Oehling
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D000943:Antigens, Differentiation / Q000096:biosynthesis; D000944:Antigens, Differentiation, B-Lymphocyte / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism; D002846:Chromatography, Affinity; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology; D007073:Immunoglobulin E / Q000378:metabolism*; D008222:Lymphokines / Q000302:isolation & purification / Q000494:pharmacology*; D011961:Receptors, Fc / Q000096:biosynthesis*; D017455:Receptors, IgE; D017452:Receptors, IgG; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000032:analysis
D000943:Antigens, Differentiation; D000944:Antigens, Differentiation, B-Lymphocyte; D008222:Lymphokines; D011961:Receptors, Fc; D017455:Receptors, IgE; D017452:Receptors, IgG; D007073:Immunoglobulin E