Evolutionary stability of fibrinogen epitopes implicated in fibrin polymerization and in fibrinolysis.
In this work, fibrinogen evolution was analysed by testing the reactivity of fibrinogen from different species with monoclonal antibodies against human fibrinogen fragment D. One epitope concerning the fibrin polymerization site 'a' and two epitopes responsible for tPA binding to fibrin were conserved in all mammalian fibrogens tested but not in crab coagulogen or pleurodella fibrinogen. In these two species, some epitopes which are not implicated in fibrinogen function were conserved. Therefore, we can conclude that polymerizing site 'a' and tPA binding sites have not been modified for at least 80 million years.
Mirshahi
M
M
U. 86, INSERM, Faculté de Médecine, Paris VI, France.
Mirshahi
S S
SS
Soria
C
C
Perrot
J Y
JY
Guidalia
M
M
Boucheix
C
C
Soria
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D005075:Biological Evolution; D001798:Blood Proteins / Q000276:immunology; D003429:Cross Reactions; D005337:Fibrin / Q000378:metabolism*; D005338:Fibrin Fibrinogen Degradation Products / Q000276:immunology; D005340:Fibrinogen / Q000235:genetics / Q000276:immunology*; D005342:Fibrinolysis; D006737:Horseshoe Crabs / Q000235:genetics / Q000276:immunology; D006801:Humans; D008322:Mammals / Q000235:genetics / Q000276:immunology; D012455:Salamandridae / Q000235:genetics; D013045:Species Specificity; D010959:Tissue Plasminogen Activator / Q000378:metabolism
D000911:Antibodies, Monoclonal; D001798:Blood Proteins; D005338:Fibrin Fibrinogen Degradation Products; C013445:coagulogen; C015214:fibrinogen D fragment; D005337:Fibrin; D005340:Fibrinogen; D010959:Tissue Plasminogen Activator