Gene sequence, developmental expression, and protein phosphorylation of RAB-17 in maize.
The ABA-induced MA12 cDNA from maize, which encodes a set of highly phosphorylated embryo proteins, was used to isolate the corresponding genomic clone. This gene, called RAB-17 (responsive to ABA), encodes a basic, glycine-rich protein (mol. wt. 17,164) containing a cluster of 8 serine residues, seven of them contiguous. It is a homologue of the rice RAB-21 gene (Mundy J, Chua NH, EMBO J 7; 2279-2286, 1988). Phosphoamino acid analysis of the isolated protein indicates that only the serine residues are phosphorylated and a putative casein-type kinase phosphorylatable sequence was identified in the protein. The pattern of expression and in vivo phosphorylation of the RAB-17 protein was studied during maize embryo germination and in calli of both meristematic or embryonic origin. ABA treatment induced the synthesis of RAB-17 mRNA and protein in calli, however, the RAB-17 proteins were found to be highly phosphorylated only in embryos.
Vilardell
J
J
Departamento de Genética Molecular, Centro de Investigación y Desarrollo, C.S.I.C., Barcelona, Spain.
Goday
A
A
Freire
M A
MA
Torrent
M
M
Martínez
M C
MC
Torné
J M
JM
Pagès
M
M
D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D010766:Phosphorylation; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D010944:Plants / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D003313:Zea mays / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
D010940:Plant Proteins; D012333:RNA, Messenger; D000040:Abscisic Acid; D004247:DNA