Cultured Kaposi's sarcoma-derived cells express functional PDGF A-type and B-type receptors.
We have investigated the role of platelet-derived growth factor (PDGF) for the growth of cultured Kaposi's sarcoma (KS)-derived cells. They were found to bind 125I-PDGF-AA and 125I-PDGF-BB in a manner which is consistent with the presence of both PDGF A-type and PDGF B-type receptors. Furthermore, KS cells responded mitogenically to both PDGF homodimers. This shows that KS cells have functional A- and B-type PDGF receptors, suggesting an important role of PDGF for the growth of KS cells in vitro. In addition we found that KS cells produce PDGF-A chain mRNA in response to PDGF stimulation. This suggests that a PDGF-induced autocrine stimulation involving PDGF-AA which could amplify the response to PDGF occurs in these cells.
Werner
S
S
Department of Virus Research, Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany.
Hofschneider
P H
PH
Heldin
C H
CH
Ostman
A
A
Roth
W K
WK
D001667:Binding, Competitive; D015152:Blotting, Northern; D002455:Cell Division / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007700:Kinetics; D010982:Platelet-Derived Growth Factor / Q000378:metabolism* / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D017479:Receptors, Platelet-Derived Growth Factor; D012514:Sarcoma, Kaposi / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism*
D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017479:Receptors, Platelet-Derived Growth Factor