Edema formation and cellular alterations following spinal cord injury in the rat and their modification with p-chlorophenylalanine.
The possibility that serotonin can modify the early pathological sequences occurring in spinal cord trauma was investigated in a rat model. To that end we took advantage of the possibility of influencing serotonin pharmacologically by treating animals with a serotonin synthesis inhibitor, p-chlorophenylalanine (p-CPA) before the production of the injury and compared the results with injured, untreated controls. A unilateral incision was made into the dorsal horn of the lower thoracic cord (about 2.5 mm deep, 4.5 mm long) and the rats were allowed to survive up to 5 h after the trauma. The injured region from untreated animals showed macroscopically at that time a pronounced swelling and the water content had increased by 3.5% as compared to intact controls. The segments rostral and caudal to the lesion also exhibited a profound increase in water content. Light microscopy revealed a significant expansion of the spinal cord as compared to controls. The swelling was most pronounced in the gray matter on the injured side. Electron microscopy showed distorted neurons, swollen astrocytes and extracellular edema in the gray matter in and around the primary lesion. There was also a sponginess in the surrounding white matter with disruption of myelin, collapsed axons and widened periaxonal spaces. Pretreatment of the rats with p-CPA significantly reduced the swelling of the injured spinal cord and there was no visible expansion. The ipsilateral edema in the central gray matter was considerable less pronounced as compared to that in untreated animals. The increase in water content was less than 1% in these animals.(ABSTRACT TRUNCATED AT 250 WORDS)
Sharma
H S
HS
Institute of Pathology, University Hospital, Uppsala, Sweden.
Olsson
Y
Y
D000818:Animals; D004487:Edema / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D010134:Fenclonine / Q000627:therapeutic use*; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism / Q000502:physiology*; D013118:Spinal Cord Diseases / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D013119:Spinal Cord Injuries / Q000150:complications* / Q000378:metabolism / Q000473:pathology
D012701:Serotonin; D010134:Fenclonine