Analysis of immunoglobulin and T-cell receptor gene rearrangements in human fetal bone marrow B lineage cells.
Fetal bone marrow B lineage cells representing multiple stages of B cell development were isolated by two-color cell sorting and analyzed for immunoglobulin H and T-cell receptor (TCR) gamma and delta gene rearrangements. Analysis of CD10+/surface mu- cells using a JH probe revealed a high frequency of rearrangements; some of these rearrangements used the 3' D region gene DQ52. Analysis of CD10+/surface mu- cells revealed no detectable TCR-gamma or -delta rearrangements, nor were TCR-delta rearrangements detected in CD10+/surface mu+ cells, despite the limited repertoire of these genes. These observations are surprising given the high frequency of TCR delta/gamma rearrangements in B cell precursor acute lymphoblastic leukemia, and identify a potential difference in patterns of gene rearrangement that distinguish normal and leukemic B cell precursors.
LeBien
T W
TW
Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis 55455.
Elstrom
R L
RL
Moseley
M
M
Kersey
J H
JH
Griesinger
F
F
D001402:B-Lymphocytes / Q000166:cytology*; D015139:Blotting, Southern; D001854:Bone Marrow Cells; D002460:Cell Line; D015321:Gene Rearrangement / Q000235:genetics*; D015329:Gene Rearrangement, T-Lymphocyte / Q000235:genetics*; D005803:Genes, Immunoglobulin / Q000235:genetics*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D016692:Receptors, Antigen, T-Cell, gamma-delta
D007143:Immunoglobulin Heavy Chains; D011948:Receptors, Antigen, T-Cell; D016692:Receptors, Antigen, T-Cell, gamma-delta