Paradoxical effects of recombinant mouse beta-interferon on different expression levels of intracisternal A particle genes.
We have studied the effect of mouse recombinant beta-Interferon on the expression of the intracisternal A particle (IAP) genes in a transformed mouse fibroblast cell line. Northern and immunoprecipitation analysis showed an increase in specific transcripts and polypeptides. Run-on experiments on isolated nuclei and transfection assays with an IAP long terminal repeat construct, coupled with the chloramphenicol acetyl transferase gene, showed no enhancement of the transcription rate following beta-IFN treatment. This suggests that the increase in IAP expression depends on a post-transcriptional event. Electron microscopic analysis revealed that in spite of the higher levels of IAP related polypeptides, the number of particles was significantly reduced by interferon.
Mercier
G
G
Laboratoire Rétrovirus et Rétrotransposons des Vertébrés, UPR A0043 CNRS, Hôpital Saint-Louis, Paris, France.
Lasneret
J
J
Garcette
M
M
Emanoil-Ravier
R
R
Peries
J
J
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects*; D007426:Genes, Intracisternal A-Particle; D007370:Interferon Type I / Q000494:pharmacology*; D051379:Mice; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000032:analysis; D011994:Recombinant Proteins; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
D007370:Interferon Type I; D011518:Proto-Oncogene Proteins; D011994:Recombinant Proteins; D003513:Cycloheximide