Atrial natriuretic peptide (ANP) does not inhibit the basal vascular tone present in the in situ blood-perfused dog gracilis muscle.
This study evaluated the effects of rat ANP(5-28) infusion into the blood-perfused dog gracilis muscle at concentrations ranging from 30 to 10,000 pg/ml. The vasculature of gracilis muscles from anesthetized beagle dogs was isolated and pump-perfused at constant flow with blood utilizing an extracorporeal circuit. Maximal vasodilatory capacity was determined by adenosine injection. ANP was infused into the arterial circuit to produce increasing arterial blood concentrations. Each infusion lasted 10 min. Systemic arterial pressure, central venous pressure, cardiac output and heart rate did not change during ANP infusion into the gracilis vasculature. ANP at arterial blood concentrations up to 10,000 pg/ml did not produce significant vasodilation although the vasculature showed pronounced vasodilation in response to adenosine. In vitro experiments showed that ANP had much less vasorelaxant activity in dog femoral artery and saphenous vein than in rabbit aorta. Therefore, rat ANP(5-28) at concentrations within and well above physiological and pharmacological ranges does not inhibit the basal vascular tone present in the innervated, blood-perfused dog gracilis muscle in situ.
Klabunde
R E
RE
Department of Pharmacology, Abbott Laboratories, Abbott Park, Illinois 60064.
Helgren
M C
MC
Novosad
E I
EI
Opgenorth
T J
TJ
D000241:Adenosine / Q000494:pharmacology; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D001783:Blood Flow Velocity; D003714:Denervation; D004285:Dogs; D005263:Femoral Artery / Q000187:drug effects / Q000502:physiology; D008297:Male; D008729:Methoxamine / Q000494:pharmacology; D009132:Muscles / Q000098:blood supply* / Q000294:innervation; D009599:Nitroprusside / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology*; D011817:Rabbits; D012501:Saphenous Vein / Q000187:drug effects / Q000502:physiology; D013564:Sympathetic Nervous System / Q000502:physiology; D014664:Vasodilation / Q000187:drug effects*
D010446:Peptide Fragments; D009599:Nitroprusside; D009320:Atrial Natriuretic Factor; C054538:atrial natriuretic factor (5-28); D008729:Methoxamine; D000241:Adenosine