Inhibitory effects of carcinogenic tryptophan pyrolysis products on phytohemagglutinin-induced blast transformation of human lymphocytes.
In order to evaluate the effect of carcinogenic heterocyclic amines on the stimulus-reaction system of human cells, we examined the effect of 5 kinds of carcinogenic heterocyclic amines including 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 2-amino-6-methyldipyrido[1,2-a:3',2'-d]imidazole (Glu-P-1), 2-amino-dipyrido[1,2-a:3',2'-d]imidazole (Glu-P-2), 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) on human lymphocyte (4 x 10(5) cells/ml) blast transformation induced by 0.125% phytohemagglutinin (PHA). Both Trp-P-1 and Trp-P-2 exhibited an inhibitory effect on the lymphocyte proliferation stimulated by PHA at the final concentration of 1.0-5.0 and 1.0-10 microM, respectively. The concentrations of Trp-P-1 and Trp-P-2 causing 50% inhibition were 3.1 and 5.1 microM, respectively. However, the other carcinogenic heterocyclic amines examined did not show any inhibitory effect on lymphocyte mitogenesis even at a final concentration of 10 microM. Viabilities of lymphocytes were more than 90% (n = 3 x 5) when 5 kinds of carcinogenic heterocyclic amines (0.1 ml) were respectively added at the final concentration of 10 microM to the lymphocyte culture medium (4 x 10(5) cells/0.9 ml).
Yanagisawa
H
H
Department of Hygiene and Preventive Medicine, Faculty of Medicine, University of Tokyo, Japan.
Wada
O
O
D000588:Amines / Q000494:pharmacology*; D002243:Carbolines / Q000494:pharmacology; D002273:Carcinogens / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006358:Hot Temperature; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D007959:Lymphocyte Culture Test, Mixed; D008433:Mathematics; D010835:Phytohemagglutinins / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D014364:Tryptophan / Q000494:pharmacology*
D000588:Amines; D002243:Carbolines; D002273:Carcinogens; D007093:Imidazoles; D010835:Phytohemagglutinins; C023785:2-amino-6-methyldipyrido(1,2-a-3',2'-d)imidazole; C019133:3-amino-1-methyl-5H-pyrido(4,3-b)indole; D014364:Tryptophan; C019132:3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole; C023847:Glu-P-2