Construction of yeast tRNA(Tyr) derivatives lacking in large part of the molecule and their tyrosine acceptance.
Derivatives of yeast tRNA(Tyr) lacking in the anticodon-arm (and D-arm) were constructed by a combination of partial digestion with RNase T1 and joining with T4 RNA ligase. Aminoacylation analyses of these derivatives ("3/4 molecule" or "1/2 molecule") showed that sufficient information for binding to TyrRS is contained mainly in the aminoacyl-stem (and T psi C-arm) of yeast tRNA(Tyr), but further information, possibly in the anticodon-arm, is necessary for efficient acceptance of tyrosine.
Yoshinari
S
S
Department of Molecular Biology, School of Science, Nagoya University, Japan.
Nishikawa
K
K
D000215:Acylation; D000926:Anticodon; D011130:RNA Ligase (ATP) / Q000378:metabolism; D012365:RNA, Transfer, Tyr / Q000737:chemistry* / Q000378:metabolism; D006163:Ribonuclease T1 / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism*; D015003:Yeasts / Q000235:genetics*
D000926:Anticodon; D012365:RNA, Transfer, Tyr; D014443:Tyrosine; D006163:Ribonuclease T1; D011130:RNA Ligase (ATP)