[Tumor cell proteins, detected using antibodies to the S- and N-terminal fragments of the fos proto-oncogene product].
Antibodies to c-fos oncoprotein were produced in rabbits by immunization with synthetic peptides, corresponding to the sequences 6-15 of N-end and 371-380 of C-end of c-fos oncoprotein. C-fos expression was tested with immunoprecipitation and immunoblotting in various transformed cell lines with antibodies to N- and C-decapeptides. It was shown that antibodies to C-terminal decapeptide revealed a c-fos gene product and also some fos-related antigens FRAs 36 kD, 46 kD, 75 kD and 90 kD in rat pheochromocytoma PC-12 cells and mouse carcinoma cell lines MAC-3 and LL. In some cell lines 46 kD FRA was expressed in the absence of p62 c-fos. Besides, different clones of the same cell line cultivated in identical conditions revealed differences in the 46 kD FRA expression. Antibodies to sequence 6-15 of N-end revealed only c-fos products and no FRAs were detected. Therefore FRAs have homology with the c-fos product in the C-terminal region and differ from it in the N-terminal region.
Mazurenko
N N
NN
Kiseleva
N P
NP
Kudriavets
Iu I
IuI
Mikhaleva
I I
II
Antonenko
V V
VV
Kiselev
F L
FL
D000818:Animals; D000912:Antibodies, Neoplasm / Q000276:immunology*; D015153:Blotting, Western; D002471:Cell Transformation, Neoplastic; D004268:DNA-Binding Proteins / Q000276:immunology*; D009363:Neoplasm Proteins / Q000032:analysis*; D011233:Precipitin Tests; D011518:Proto-Oncogene Proteins / Q000276:immunology*; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D051381:Rats; D014407:Tumor Cells, Cultured
D000912:Antibodies, Neoplasm; D004268:DNA-Binding Proteins; D009363:Neoplasm Proteins; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger
Belki opukholevykh kletok, vyiavliaemye s pomoshch'iu antitel k S- i N-kontsevym fragmentam produkta protoonkogena fos.