A new and rapid method for the assay of bacterial fatty acids using high resolution capillary gas chromatography and trimethylsulfonium hydroxide.
Gas-liquid chromatography of cellular fatty acids is a useful tool for the identification of bacteria. Derivatization of bacterial fatty acids to methyl esters by conventional techniques is usually time-consuming and complicated. A new one-step technique using trimethyl-sulfonium hydroxide allows the direct formation of fatty acid methyl esters within 1-2 min. Some random examples of profiles demonstrate that straight, branched, saturated, unsaturated, hydroxy and cyclopropyl fatty acids match conventional preparations well. The method is a very sensitive one, since only a few colonies are sufficient for preparation of fatty acid methyl esters.
Müller
K D
KD
Institut für Medizinische Mikrobiologie der Universität (GHS) Essen.
Husmann
H
H
Nalik
H P
HP
D000333:Aeromonas / Q000032:analysis / Q000145:classification; D001412:Bacillus subtilis / Q000032:analysis / Q000145:classification; D001419:Bacteria / Q000032:analysis* / Q000145:classification; D002849:Chromatography, Gas; D004952:Esters; D005227:Fatty Acids / Q000032:analysis*; D008745:Methylation; D011550:Pseudomonas aeruginosa / Q000032:analysis / Q000145:classification; D013211:Staphylococcus aureus / Q000032:analysis / Q000145:classification; D013452:Sulfonium Compounds
D004952:Esters; D005227:Fatty Acids; D013452:Sulfonium Compounds; C009581:trimethylsulfonium