The gene encoding SMR1, a precursor-like polypeptide of the male rat submaxillary gland, has the same organization as the preprothyrotropin-releasing hormone gene.
SMR1 is a precursor-like polypeptide of the submaxillary glands of rats. Sequence analysis predicts that it could be processed by maturation enzymes to release a small peptide resembling the thyrotropin-releasing hormone. The SMR1 gene was isolated from a rat genomic library and sequenced. The SMR1 gene spans 4.7 kb and consists of three exons. The two introns occur a few nucleotides before the initiation codon in the 5' untranslated region, and a few nucleotides before the first predicted processing site, respectively. Such a structure is reminiscent of that of the preprothyrotropin-releasing hormone gene. The site of transcriptional initiation of the SMR1 gene was determined and 1.4 kb of 5'-flanking sequence was sequenced. The sequence analysis revealed the presence of alternating purine-pyrimidine tracts and of purine-rich sequences. In addition, some sequences which could be involved in the regulation of SMR1 gene expression were identified.
Rosinski-Chupin
I
I
Département d'Immunologie, Institut Pasteur, Paris, France.
Rougeon
F
F
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005786:Gene Expression Regulation / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D012471:Salivary Proteins and Peptides / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013363:Submandibular Gland / Q000378:metabolism*; D013973:Thyrotropin-Releasing Hormone / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics
D011498:Protein Precursors; D012471:Salivary Proteins and Peptides; C066861:Smr3b protein, rat; C063596:prepro-thyrotropin-releasing hormone; D013973:Thyrotropin-Releasing Hormone