Structural relationship between the enzymatic and streptococcal binding sites of human salivary alpha-amylase.
Previous studies have demonstrated that human salivary alpha-amylase specifically binds to the oral bacterium Streptococcus gordonii. This interaction is inhibited by substrates such as starch and maltotriose suggesting that bacterial binding may involve the enzymatic site of amylase. Experiments were performed to determine if amylase bound to the bacterial surface possessed enzymatic activity. It was found that over one-half of the bound amylase was enzymatically active. In addition, bacterial-bound amylase hydrolyzed starch to glucose which was then metabolized to lactic acid by the bacteria. In further studies, the role of amylase's histidine residues in streptococcal binding and enzymatic function was assessed after their selective modification with diethyl pyrocarbonate. DEP-modified amylase showed a marked reduction in both enzymatic and streptococcal binding activities. These effects were diminished when DEP modification occurred in the presence of maltotriose. DEP-modified amylase had a significantly altered secondary structure when compared with native enzyme or amylase modified in the presence of maltotriose. Collectively, these results suggest that human salivary alpha-amylase may possess multiple sites for bacterial binding and enzymatic activity which share structural similarities.
Scannapieco
F A
FA
Department of Oral Biology, School of Dental Medicine, State University of New York, Buffalo 14214.
Bhandary
K
K
Ramasubbu
N
N
Levine
M J
MJ
D000143:Acids / Q000378:metabolism; D001665:Binding Sites / Q000187:drug effects; D002942:Circular Dichroism; D004047:Diethyl Pyrocarbonate / Q000494:pharmacology; D006801:Humans; D006868:Hydrolysis; D005779:Immunodiffusion; D011487:Protein Conformation; D012463:Saliva / Q000201:enzymology*; D013213:Starch / Q000378:metabolism; D013291:Streptococcus / Q000378:metabolism*; D013379:Substrate Specificity; D014312:Trisaccharides / Q000378:metabolism; D000516:alpha-Amylases / Q000378:metabolism*
D000143:Acids; D014312:Trisaccharides; C008317:maltotriose; D013213:Starch; D000516:alpha-Amylases; D004047:Diethyl Pyrocarbonate