Human immunodeficiency virus vpr gene encodes a virion-associated protein.
The vpr gene of human immunodeficiency virus type 1 (HIV-1) is one of the seven accessory genes that are believed to have roles in the virus replication cycle. We report here the detection of a 13 kD vpr protein in sucrose gradient-purified HIV-1. This protein was not detected in cells infected with a virus having a truncated vpr gene that lacks the potential to encode for 26 C-terminal amino acid residues. These findings raise the possibility that virion-associated vpr proteins may be involved in the early life cycle of HIV-1 replication and suggest that the C-terminal region of the vpr gene is essential for its expression.
Yuan
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Department of Cancer Biology, Harvard University School of Public Health, Boston, MA 02115.
Matsuda
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Matsuda
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Essex
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Lee
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TH
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D015967:Gene Expression Regulation, Viral; D016336:Gene Products, vpr / Q000235:genetics*; D005814:Genes, Viral; D015658:HIV Infections / Q000382:microbiology; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics*; D014771:Virion / Q000235:genetics; D014779:Virus Replication; D054325:vpr Gene Products, Human Immunodeficiency Virus
D016336:Gene Products, vpr; D011994:Recombinant Proteins; D014764:Viral Proteins; D054325:vpr Gene Products, Human Immunodeficiency Virus