D variant of encephalomyocarditis virus (EMCV-D)-induced diabetes following natural killer cell depletion in diabetes-resistant male C57BL/6J mice.
The involvement of natural killer (NK) cells in the development of diabetes in the normally resistant 9-10 week old C57BL/6J male mice by the D variant of encephalomyocarditis virus (EMCV-D) was examined. Inoculation of purified EMCV-D induced maximum NK cell activity in splenic cell populations on day 4 post-inoculation as determined by lysis of YAC-1 target cells in a standard 51chromium release microcytotoxicity assay. Selective depletion of NK cells by the administration of rabbit anti-asialo GM1 sera prior to challenging the C57BL/6J mice with EMCV-D, resulted in diminished splenic NK cell activity, increased EMCV-D viral titers in the pancreas, spleen, heart and brain, and the induction of diabetes in 60-80% of the mice. The data suggest that NK cells play a role in host protection against the diabetogenic EMCV-D.
White
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Department of Microbiology and Immunology, College of Science & Mathematics, Wright State University, Dayton, OH.
Smith
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RA
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000276:immunology / Q000382:microbiology*; D004686:Encephalomyocarditis virus / Q000276:immunology / Q000472:pathogenicity*; D004769:Enterovirus Infections / Q000276:immunology*; D005677:G(M1) Ganglioside; D005951:Glucose Tolerance Test; D006028:Glycosphingolipids / Q000276:immunology; D007106:Immune Sera / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010179:Pancreas / Q000382:microbiology; D014779:Virus Replication
D006028:Glycosphingolipids; D007106:Immune Sera; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside