title: Phi X174 protein E-mediated lysis of Escherichia coli.
abstract: Bacteriophage PhiX174 encodes a single lysis gene, E, the function of which is necessary and sufficient to induce lysis of Escherichia coli. Here we present a novel model for E-lysis: physiological, genetic and biochemical data are presented which suggest that a transmembrane tunnel penetrating the inner and outer membrane is formed during the lytic action of protein E. Moreover, using high magnification scanning and transmission electron microscopy in this study, it was possible to visualize the transmembrane lysis structure directly.
authors: Institute of Microbiology and Genetics, University of Vienna, Austria.
authors: A
authors: A
authors: Witte
authors: U
authors: U
authors: Bläsi
authors: G
authors: G
authors: Halfmann
authors: M
authors: M
authors: Szostak
authors: G
authors: G
authors: Wanner
authors: W
authors: W
authors: Lubitz
mesh_terms: D001433:Bacteriolysis; D010584:Bacteriophage phi X 174 / Q000235:genetics / Q000502:physiology*; D002462:Cell Membrane / Q000648:ultrastructure; D004926:Escherichia coli / Q000648:ultrastructure; D005814:Genes, Viral; D008954:Models, Biological; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000502:physiology; D013329:Structure-Activity Relationship; D014764:Viral Proteins / Q000235:genetics / Q000494:pharmacology*; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: C029555:E protein, bacteriophage X174; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins