[SEM studies on IFN-receptor dynamics of cultured cells from mammals].
Visualization of rIFN-alpha 1 and -alpha 2 receptors on MDBK-cells was done by means of a two step method with gold-labelled monoclonal anti IFN-antibodies in the SEM. This binding showed a marked dependency from the temperature, concentration of the rHu-IFN, prefixation of the MDBK-cells, and time of incubation. This results are in agreement with findings of classical virological methods. By means of SEM was shown that the distribution and amount of the IFN-receptors on MDBK-cells depends strongly on the cell cycle and that not each was labelled. The results were discussed in connection with structural-functional alterations of the cytoskeleton.
Augsten
K
K
Zentralinstitut für Mikrobilogie und experimentelle Therapie, Akademie der Wissenschaften der DDR, Jena.
Unger
E
E
Tonew
M
M
Wollweber
L
L
Noll
F
F
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D002453:Cell Cycle; D002460:Cell Line; D006801:Humans; D007370:Interferon Type I / Q000378:metabolism*; D007668:Kidney; D008855:Microscopy, Electron, Scanning; D011971:Receptors, Immunologic / Q000378:metabolism* / Q000648:ultrastructure; D017471:Receptors, Interferon; D011994:Recombinant Proteins
D000911:Antibodies, Monoclonal; D007370:Interferon Type I; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D011994:Recombinant Proteins
REM-Untersuchungen zur IFN-Rezeptordynamik von Säugerkulturzellen.