title: Interleukin-2 driven nuclear translocation of prolactin in cloned T-lymphocytes.
abstract: The nuclear translocation of PRL is demonstrated at the immunofluorescence and electron microscopic (EM) levels in interleukin-2 (IL2)-stimulated cloned T-cells and concanavalin A-stimulated splenocytes. This translocation occurs 2-10 h after IL2 stimulation, and is reversed by the addition of anti-PRL antiserum into the extracellular culture medium. The nuclear localization of PRL in IL2 stimulated T-cells was confirmed by postembedding immunogold EM. The nuclear uptake of PRL after IL2 stimulation was further documented by EM studies using PRL-colloidal gold conjugates. These studies suggest that the intranuclear PRL is translocated from the extracellular medium via an endosomal/lysosomal pathway over a period of several hours. Finally, the requirement for PRL no later than 6 h after IL2 stimulation is demonstrated through the reversible inhibition of T-cell growth with anti-PRL antiserum.
authors: Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia 19104.
authors: C V
authors: CV
authors: Clevenger
authors: A L
authors: AL
authors: Sillman
authors: M B
authors: MB
authors: Prystowsky
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D002999:Clone Cells; D005455:Fluorescent Antibody Technique; D007376:Interleukin-2 / Q000494:pharmacology*; D051379:Mice; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D011388:Prolactin / Q000378:metabolism*; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
chemical_list: D007376:Interleukin-2; D011388:Prolactin