Heterogeneity of protein kinase C expression and regulation in T lymphocytes.
The purpose of the present study was to examine protein kinase C (PKC) isotype expression in T lymphoblasts derived from peripheral blood and the T leukaemic cell Jurkat. Using antisera reactive with PKC alpha, beta 1, and beta 2 and gamma, it was observed that T cells expressed two PKC isotypes, PKC alpha and beta 1. No PKC gamma was detected in T lymphocytes. In lymphoblasts, high levels of PKC beta compared to PKC alpha were found whereas Jurkat cells expressed high levels of alpha compared to PKC beta. Differences in the calcium sensitivity of phorbol ester-induced phosphorylation were observed in Jurkat and T lymphoblasts which correlated with the relative levels of PKC alpha and beta isotypes expressed by the cells.
Lucas
S
S
Lymphocyte Activation Laboratory, Imperial Cancer Research Fund, London, England.
Marais
R
R
Graves
J D
JD
Alexander
D
D
Parker
P
P
Cantrell
D A
DA
D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D001921:Brain / Q000201:enzymology; D017252:CD3 Complex; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line / Q000187:drug effects; D002463:Cell Membrane Permeability; D004789:Enzyme Activation / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D015458:Leukemia, T-Cell / Q000201:enzymology; D008213:Lymphocyte Activation; D010766:Phosphorylation; D011493:Protein Kinase C / Q000032:analysis / Q000096:biosynthesis*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000032:analysis / Q000201:enzymology* / Q000276:immunology
D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D011948:Receptors, Antigen, T-Cell; D011493:Protein Kinase C; D002118:Calcium