Effect of retinoic acid on two glycosyltransferase activities in C6 cultured glioma cells.
1. Activity of two glycosyltransferases was studied in retinoic acid-treated C6 cultured glioma cells. 2. The beta-galactoside alpha 2,3-sialyltransferase transferring N-acetylneuramin onto the O-glycans residues of glycoproteins was activated up to twice after chronic treatment (from 24 to 96 hr) with all-trans retinoic acid. 3. No effect was observed for shorter treatments. 4. On the opposite, the N-glycan galactosyltransferase activity remained unchanged whatever the length of retinoic acid treatment was. 5. The activatory effect was not dependent on isomery, as all-trans and 13-cis retinoic acid isomers were both activators of the C6 glioma cell sialyltransferase. 6. Measurement of adhesion of retinoic acid-treated cells using labelled plasma membranes showed an enhancement of adhesion in correlation with enhancement of sialyltransferase activity.
Reboul
P
P
INSERM U.189, Laboratoire de Biochimie Générale et Médicale, Faculté de Médecine Lyon-Sud, Oullins, France.
Broquet
P
P
George
P
P
Louisot
P
P
D000818:Animals; D001921:Brain / Q000201:enzymology; D002448:Cell Adhesion / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D005700:Galactosyltransferases / Q000378:metabolism*; D005910:Glioma / Q000201:enzymology*; D007536:Isomerism; D007700:Kinetics; D051381:Rats; D012799:Sialyltransferases / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
D014212:Tretinoin; D005700:Galactosyltransferases; D012799:Sialyltransferases; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase