Enzymatic activities related to intermediary metabolism of glucose in circulating mononuclear cells from obese humans: relationship to enzyme activity in adipose tissue.
In order to assess whether enzyme activities of glucose metabolism measured in mononuclear blood cells reflect those in a typical insulin target tissue, we studied hexokinase, 6-phosphofructokinase, glucose-6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase activities in lymphomonocytes and in hypogastric adipose tissue from 15 nondiabetic obese women. Statistically significant relationships were found in the activities of hexokinase (r = 0.53, p less than 0.05), 6-phosphofructokinase (r = 0.85, p less than 0.01), and 6-phosphogluconate dehydrogenase (r = 0.72, p less than 0.01) between the two tissues. These results suggest that mononuclear blood cells may be suitable as a model for studying cytosolic key enzymes involved in the glucose metabolism of humans.
Moghetti
P
P
Chair of Metabolic Diseases, University of Verona Medical School, Italy.
Bonora
E
E
Cigolini
M
M
Querena
M
M
Cacciatori
V
V
Muggeo
M
M
D000273:Adipose Tissue / Q000201:enzymology*; D000328:Adult; D001786:Blood Glucose / Q000378:metabolism*; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000097:blood; D006593:Hexokinase / Q000097:blood; D006801:Humans; D007700:Kinetics; D009000:Monocytes / Q000201:enzymology*; D009765:Obesity / Q000097:blood / Q000201:enzymology*; D010732:Phosphofructokinase-1 / Q000097:blood; D010734:Phosphogluconate Dehydrogenase / Q000097:blood
D001786:Blood Glucose; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase; D010732:Phosphofructokinase-1