[Comparative study of 2 rapid techniques (DNA hybridization and chromatography) for the identification of Mycobacterium tuberculosis complex isolated from clinical specimens].
We have compared the capacity of two combined chromatographic methods (gas chromatography and thin-layer chromatography) and a nucleic acid hybridization probe (Gen-Probe) to identify members of the Mycobacterium tuberculosis complex. The results of a single test show 6% of false-negatives with Gen-Probe and 8.5% doubtfuls with chromatography. After repeated, the specificity of both methods was improved to 100%. The advantages and disadvantages of the two methods in relation to its capacity to provide a rapid identification of Mycobacterium tuberculosis are discussed.
Artero
J M
JM
Servicio de Microbiología, Hospital General de Murcia.
Herrera
E A
EA
Valero
P L
PL
Martín Luengo
F
F
D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D015342:DNA Probes; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D009171:Mycolic Acids / Q000032:analysis; D009693:Nucleic Acid Hybridization
D015342:DNA Probes; D009171:Mycolic Acids
Estudio comparativo de dos técnicas rápidas (hibridación de ADN y cromatografía) para la identificación de Mycobacterium tuberculosis complex aislados de muestras clínicas.