Microsomal N-hydroxylation of the glycolamide 2-(glycolylamino)fluorene to give the glycolylhydroxamic acid. A new xenobiotic reaction.
The glycolamide 2-(glycolylamino)fluorene was found to be metabolized in part by induced rat liver microsomes to the hydroxamic acid N-hydroxy-2-(glycolylamino)fluorene. This is the first report of the ability of a microsomal system to carry out the N-hydroxylation of a glycolamide. A comparison of the relative rates of metabolism of the acetyl and glycolyl amides of 2-aminofluorene showed that the former gave about twice as much of the hydroxamic acid as did the latter. On the other hand, the overall metabolism of the glycolamide was slightly more rapid than that of the acetyl congener. Both the glycolyl- and acetyl-derived hydroxamic acids were further metabolized to unknown products by microsomal preparations in the presence of NADPH.
Corbett
M D
MD
Food Science and Human Nutrition Department, University of Florida, Gainesville 32611.
Corbett
B R
BR
Quintana
S J
SJ
Hannothiaux
M H
MH
Wei
C I
CI
D000577:Amides / Q000378:metabolism; D000818:Animals; D001711:Biotransformation; D005449:Fluorenes / Q000378:metabolism; D006877:Hydroxamic Acids / Q000378:metabolism*; D006900:Hydroxylation; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D015262:Xenobiotics / Q000378:metabolism*
D000577:Amides; D005449:Fluorenes; D006877:Hydroxamic Acids; D015262:Xenobiotics; C012177:2-aminofluorene