Chondroitin sulfate proteoglycan in the extracellular matrix of the canine superior olivary nuclei.
The perineuronal extracellular matrix of the canine superior olivary nuclei was examined by the histochemical method. The extracellular matrix was stained with Alcian blue (pH 1.0 and 2.5), high iron diamine and ruthenium red. The staining intensity of Alcian blue in the extracellular matrix was remarkably reduced after chondroitinase ABC digestion but not after that of heparitinase or hyaluronidase. These results indicate that the extracellular matrix consists of proteoglycans and contains the chondroitin sulfate proteoglycan.
Atoji
Y
Y
Department of Veterinary Anatomy, Faculty of Agriculture, Gifu University, Japan.
Kitamura
Y
Y
Suzuki
Y
Y
D000818:Animals; D002809:Chondroitin Sulfates / Q000032:analysis* / Q000378:metabolism; D002810:Chondroitinases and Chondroitin Lyases / Q000494:pharmacology; D004285:Dogs; D005109:Extracellular Matrix / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D006651:Histocytochemistry; D008854:Microscopy, Electron; D009847:Olivary Nucleus / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism
D002809:Chondroitin Sulfates; D002810:Chondroitinases and Chondroitin Lyases