Nucleotide sequence and transcriptional analysis of the Escherichia coli agp gene encoding periplasmic acid glucose-1-phosphatase.
The nucleotide sequence of the agp gene, which encodes a periplasmic glucose-1-phosphatase, was determined. The deduced amino acid sequence corresponds to a 413-amino-acid-residue polypeptide with a typical hydrophobic signal sequence of 22 amino acids. The mature protein lacks the N-terminal signal peptide and has a calculated Mr of 43,514. Its promoter was defined by primer extension of the mRNA made in vivo. Like many genes under positive control, its -35 promoter region does not match the consensus. The agp gene is both preceded and followed by transcription termination signals, so it appears to be transcribed as a single unit.
Pradel
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Departement de Biologie, Centre d'Etudes Nucléaires de Saclay, Gif-sur-Yvette, France.
Marck
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Boquet
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PL
D000595:Amino Acid Sequence; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D012333:RNA, Messenger; C062887:agp protein, E coli; D010744:Phosphoric Monoester Hydrolases