Study of the sensitivity of blood spotted on filter paper in the detection of HBsAg and anticore using ELISA technique.
The sensitivity of the filter paper method was compared with that of whole serum in the detection of HBsAg and antic using ELISA technique. It was found that analysis of eluates of blood spotted on filter paper should be recommended in field surveys and of HBsAg screening prior to an immunization campaign. On the other hand, in the detection of anti HBc the eluted technique is less sensitive and should not be performed if accurate epidemiological data are to be collected.
Farghaly
A M
AM
Tropical Health Department, Alexandria University.
Kotkat
A M
AM
D001800:Blood Specimen Collection / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005374:Filtration; D006512:Hepatitis B Core Antigens / Q000097:blood* / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006801:Humans; D010209:Paper; D012680:Sensitivity and Specificity
D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens