title: Autoantibodies to nucleolar antigens in systemic scleroderma: clinical correlations.
abstract: Indirect immunofluorescence(IIF) and double immunodiffusion (DID) were performed on the sera of 64 patients who had a nucleolar immunofluorescence pattern on HEp-2 cells. Forty-nine of the sera were from 296 patients with systemic scleroderma (SSc) and 15 sera were from 214 patients with systemic lupus erythematosus (SLE). A homogeneous nucleolar staining pattern was found in 45 of the 64 sera (70.3%), a clumpy fluorescence associated with fibrillarin antibody in 14 (21.8%) and a speckled pattern was found in five of the sera (7.8%). There was a clear correlation between the sera which showed a homogeneous nucleolar staining pattern with symptoms of the polymyositis/scleroderma overlap syndrome that differed from SSc with concomitant myositis. The clumpy pattern was mainly associated with diffuse scleroderma and the speckled pattern with limited scleroderma (previously called acrosclerosis).
authors: Department of Dermatology, Warsaw School of Medicine, Poland.
authors: M
authors: M
authors: Błaszczyk
authors: M
authors: M
authors: Jarzabek-Chorzelska
authors: S
authors: S
authors: Jabłońska
authors: T
authors: T
authors: Chorzelski
authors: Z
authors: Z
authors: Kołacińska-Strasz
authors: E H
authors: EH
authors: Beutner
authors: V
authors: V
authors: Kumar
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000032:analysis*; D001324:Autoantigens / Q000276:immunology; D002466:Cell Nucleolus / Q000276:immunology*; D004264:DNA Topoisomerases, Type I; D005095:Exoribonucleases; D063326:Exosome Multienzyme Ribonuclease Complex; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D005779:Immunodiffusion; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D008947:Mixed Connective Tissue Disease / Q000276:immunology; D009687:Nuclear Proteins / Q000276:immunology; D012595:Scleroderma, Systemic / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D009687:Nuclear Proteins; C048100:Scl 70 antigen, human; D005095:Exoribonucleases; D063326:Exosome Multienzyme Ribonuclease Complex; C077044:EXOSC10 protein, human; D004264:DNA Topoisomerases, Type I