DNA damage induced in mouse peritoneal exudate cells after in vivo administration of chemical and physical agents as determined by alkaline elution.
The alkaline elution technique for detecting DNA strand breaks has been applied to the study of DNA damage in mouse peritoneal exudate cells resulting from the in vivo administration of chemical and physical agents. The direct methylating agents methyl methanesulphonate and N-methyl-N-nitrosourea induced extensive breakage in samples taken 2 h after administration. The direct ethylating agents ethyl methanesulphonate and N-ethyl-N-nitrosourea also induced DNA strand breaks, but to a lesser extent than the methylating agents. The indirect methylating agent dimethylnitrosamine showed hardly any effect in this system. A weak but positive response was observed upon treatment with the anti-neoplastic alkylating agent procarbazine hydrochloride. The whole-body irradiation of mice with 60Co gamma-rays also induced DNA strand breaks. The elution profiles for gamma-ray irradiation were different from those of alkylating agents, and indicate that alkylating agents produce many more secondary lesions leading to DNA strand breaks than gamma-rays. N-methyl-N-nitrosourea produced slightly more DNA strand breaks in mutagen-sensitive mice, which are derived from the CD-1 strain, than in ICR mice.
Nishi
Y
Y
Life Science Research Laboratory, Japan Tobacco Inc., Kanagawa.
Miyanaga
K
K
Sato
S
S
Inui
N
N
D000477:Alkylating Agents / Q000633:toxicity*; D000818:Animals; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005260:Female; D051379:Mice; D009152:Mutagenicity Tests / Q000379:methods*; D010529:Peritoneal Cavity / Q000166:cytology; D013047:Specific Pathogen-Free Organisms; D014466:Ultraviolet Rays
D000477:Alkylating Agents