[Inhibition of phorbol ester and A23187-stimulated prostaglandins synthesis in macrophages by anisodamine].
The effect of anisodamine on arachidonic acid metabolism in macrophages was studied. The results indicated that the synthesis of PGI2, TXA2 and PGF2 alpha stimulated by phorbol-12-myristate-13-acetate (PMA) and calcium ionophore A23187 was dose and time-dependently inhibited by anisodamine, and preincubation of the cells with anisodamine significantly decreased subsequent PMA and A23187-stimulated synthesis of prostaglandins. An isodamine failed to inhibit exogenous arachidonic acid-stimulated synthesis of prostaglandins. It is suggested that the site of action of anisodamine could be the phospholipase A2 reaction.
Xiao
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Institute of Basic Medical Sciences, Beijing.
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000001:Calcimycin / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology; D012991:Solanaceous Alkaloids / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013929:Thromboxane B2 / Q000096:biosynthesis*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D012991:Solanaceous Alkaloids; C003922:anisodamine; D000001:Calcimycin; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D013755:Tetradecanoylphorbol Acetate