Genomic location of a human cytomegalovirus protein with protein kinase activity (PK68).
A major protein of Mr 68,000 with a previously described protein kinase activity (PK68), which is induced in HCMV-infected cells, is shown to be virus encoded by means of hybrid selection. The gene coding for the corresponding 4-kb mRNA was located within map units 0.510 and 0.525. This location is the same as that of pp65, a structural protein with no attributed function. The early nature of the protein was confirmed with its mRNA first appearing 3 hr after infection under cycloheximide reversal and phosphonoacetic acid blocking conditions. Another mRNA, 97 nucleotides smaller and 5' coterminal with PK68, which codes for a Mr 52,000 protein, was also observed.
Somogyi
T
T
Unité de Virologie Médicale, Institut Pasteur, Paris, France.
Michelson
S
S
Masse
M J
MJ
D015152:Blotting, Northern; D002460:Cell Line; D003001:Cloning, Molecular; D003587:Cytomegalovirus / Q000201:enzymology / Q000235:genetics*; D005814:Genes, Viral; D006801:Humans; D009693:Nucleic Acid Hybridization; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D011494:Protein Kinases / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping; D014158:Transcription, Genetic; D015678:Viral Structural Proteins / Q000235:genetics*
D012333:RNA, Messenger; D012367:RNA, Viral; D015678:Viral Structural Proteins; D011494:Protein Kinases