Opsonic and protective activity of five human IgM monoclonal antibodies reactive with lipopolysaccharide antigen of Pseudomonas aeruginosa.
International Antigen Typing Schema (IATS) serotypes 1, 2, 5, 6, 8 and 11 comprise approximately 80% of Pseudomonas aeruginosa strains isolated from blood, wounds and respiratory specimens. Five human immunoglobulin M (IgM) monoclonal antibodies (MAbs) reactive with lipopolysaccharide O antigens of these IATS serotypes were studied in an opsonophagocytic assay. The assay employed human polymorphonuclear leukocytes, 2% guinea pig serum as the complement source and MAb. Each MAb promoted killing of inoculum of the homologous LPS serotype. The opsonic activity of each MAb was complement-dependent. In a murine model of Pseudomonas burn wound sepsis the LD50 of five strains of P. aeruginosa was increased greater than or equal to 22-fold by MAb-treatment (1.0 mg/kg). The mean effective dose of the five MAbs in mice challenged with approximately 10 LD50 of the homologous LPS serotype ranged from less than 0.01 mg/kg to 1.00 mg/kg.
Collins
M S
MS
Cutter Biological, Miles Inc., Berkeley, CA 94701.
Ladehoff
D K
DK
Mehton
N S
NS
Noonan
J S
JS
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology* / Q000627:therapeutic use; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000627:therapeutic use; D000942:Antigens, Bacterial / Q000276:immunology*; D002056:Burns / Q000150:complications; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology* / Q000627:therapeutic use; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D009895:Opsonin Proteins / Q000276:immunology*; D010587:Phagocytosis; D011552:Pseudomonas Infections / Q000209:etiology / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D018805:Sepsis / Q000209:etiology / Q000517:prevention & control
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D009895:Opsonin Proteins