Endothelin-1 and -3 induce the release of tissue-type plasminogen activator and von Willebrand factor from endothelial cells.
Stimulation of the isolated perfused rat hindleg vascular bed with various concentrations of endothelin-1 or endothelin-3 resulted in the acute release of tissue-type plasminogen activator (t-PA) and of von Willebrand factor (vWF). The release of both endothelial cell products required the presence of extracellular calcium; the release of vWF by endothelin was instantly inhibited by disodium EDTA. The calcium antagonists nifedipine, diltiazem and verapamil partially inhibited endothelin-induced t-PA release, but had no effect on endothelin-induced vWF release.
Pruis
J
J
Gaubius Institute TNO, Leiden, The Netherlands.
Emeis
J J
JJ
D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000502:physiology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D016232:Endothelins / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006614:Hindlimb / Q000098:blood supply; D066298:In Vitro Techniques; D008297:Male; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D012039:Regional Blood Flow / Q000187:drug effects; D010959:Tissue Plasminogen Activator / Q000378:metabolism*; D014841:von Willebrand Factor / Q000378:metabolism*
D002121:Calcium Channel Blockers; D016232:Endothelins; D014841:von Willebrand Factor; D010959:Tissue Plasminogen Activator; D002118:Calcium