Cloning and expression of a cDNA for the human homolog of mouse T cell and mast cell growth factor P40.
A cDNA encoding the human homolog of mouse T-cell and mast cell growth factor P40 was derived from peripheral blood mononuclear cells (PBMC) stimulated with phytohemagglutinin and phorbol myristate acetate. Sequence analysis of the cDNA predicted a precursor protein of 144 amino acids including a signal peptide of 18 residues, a structure identical with that of mouse P40. The homology between the mouse and human proteins is 55% with a perfect conservation of the 10 cysteine residues present in the mature polypeptide. Expression of the cDNA for human P40 in a baculovirus vector yielded a protein capable of enhancing in vitro survival of human T cell lines.
Renauld
J C
JC
Unit of Experimental Medicine, Université Catholique de Louvain, Belgium.
Goethals
A
A
Houssiau
F
F
Van Roost
E
E
Van Snick
J
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D006133:Growth Substances / Q000235:genetics; D006801:Humans; D016906:Interleukin-9; D007378:Interleukins / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics
D006133:Growth Substances; D016906:Interleukin-9; D007378:Interleukins; D012333:RNA, Messenger; D004247:DNA