Serological diagnosis of dengue by an ELISA inhibition method (EIM).
An ELISA Inhibition Method (EIM) was proposed for the serologic diagnosis of dengue, comparing its results with the Hemagglutination Inhibition (HI) and the IgM capture-ELISA (MAC-ELISA). Advantages and disadvantages of both methods are discussed according to sensitivity, specificity, performance and usefulness. As a conclusion we recommend the complementary inclusion of the EIM and MAC-ELISA substituting the HI for laboratories engaged in the diagnosis and surveillance of dengue.
Fernández
R J
RJ
Departamento de Virología, Instituto de Medicina Tropical "Pedro Kouri", Siboney, Ciudad de La Habana, Cuba.
Vázquez
S
S
D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D000914:Antibodies, Viral / Q000032:analysis*; D003715:Dengue / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*
D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral; D007075:Immunoglobulin M