[Use of high performance liquid chromatography for analysis of subfractions of lysine-rich histone H1 from Baikal fish--the sand sculpin Cottus kessleri Dyb].
High performance liquid chromatography using PMSS-C18 resin was applied to separate variant-complicated histone H1 from liver of a Baikal lake endemic fish sand sculpin (Cottus Kessleri Dybow). Total H1 was resolved into 3 main fractions. One of them was resolved into two sub-fractions by rechromatography. All the fractions and sub-fractions obtained were studied for their amino acid composition. One of the subfractions (H1(3] contained no tyrosine residues, but very much (20%) proline. Hence, this subfraction may be a new type of lysine-rich histone H1.
Fedorov
S P
SP
Slobodianiuk
S Ia
SIa
Pavlova
M E
ME
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005399:Fishes; D006657:Histones / Q000032:analysis*; D008239:Lysine / Q000032:analysis*
D000596:Amino Acids; D006657:Histones; D008239:Lysine
Primenenie vysokoéffektivnoĭ zhidkostnoĭ khromatografii dliia analiz subfraktsiĭ lizin-bogatogo gistona H1 baĭkal'skogo bychka-peschanoĭ shirokolobki Cottus kessleri Dyb.