The detection of lipocortins 1, 2 and 5 in central nervous system tissues from Lewis rats with acute experimental allergic encephalomyelitis.
The calcium and phospholipid-binding proteins lipocortins 1, 2 and 5 have been detected, by electrotransfer and immunoblotting techniques, in central nervous system (CNS) tissues of control Lewis rats and animals with experimental allergic encephalomyelitis (EAE). The cerebellum and spinal cord content of lipocortins 2 and 5 remained unchanged throughout the development of EAE but the amounts of the steroid-inducible protein lipocortin 1 increased in samples from pre-diseased and adjuvant-treated rats and were further enhanced in tissues from clinically sick and convalescent animals. The significance of these findings is discussed in conjunction with the ongoing changes in pathology which occur during the progression of EAE.
Bolton
C
C
Department of Pharmacy and Pharmacology, University of Bath, U.K.
Elderfield
A J
AJ
Flower
R J
RJ
D000208:Acute Disease; D000818:Animals; D017302:Annexins; D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000276:immunology; D002531:Cerebellum / Q000737:chemistry*; D003345:Corticosterone / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000378:metabolism*; D015151:Immunoblotting; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D013116:Spinal Cord / Q000737:chemistry*
D017302:Annexins; D002135:Calcium-Binding Proteins; D003345:Corticosterone