Heavy chain variable region gene utilization in human antibodies.
We have sequenced by the polymerase chain reaction the heavy chain variable regions of over thirty human antibodies of defined specificity. The distribution of VH families in this group of antibodies is disproportionately skewed toward the recently discovered VH gene families. Within a family, there is also a disproportional use of individual gene segments. Numerous explanations for these imbalances are discussed.
Pascual
V
V
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235-9048.
Andris
J
J
Capra
J D
JD
D001323:Autoantibodies; D015870:Gene Expression; D005803:Genes, Immunoglobulin; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007132:Immunoglobulin Isotypes; D007144:Immunoglobulin J-Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D005810:Multigene Family; D009154:Mutation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D001323:Autoantibodies; D007143:Immunoglobulin Heavy Chains; D007132:Immunoglobulin Isotypes; D007144:Immunoglobulin J-Chains; D007135:Immunoglobulin Variable Region