Isolation and sequence determination of frog C-type natriuretic peptide.
A new bioactive peptide was isolated from frog brain using a bioassay for chick rectum relaxant activity. Amino acid sequence of this peptide was determined to be Gly-Tyr-Ser-Arg-Gly-Cys-Phe-Gly-Val-Lys-Leu-Asp-Arg-Ile-Gly-Ala-Phe-Ser- Gly- Leu-Gly-Cys, in which two cysteines were linked by a disulfide bond. The peptide was found to belong structurally to the natriuretic peptide family and to exert diuretic-natriuretic activity as well as hypotensive activity when injected into rats. The peptide showed a high homology to recently identified porcine C-type natriuretic peptide (CNP) and a pharmacological spectrum highly similar to porcine CNP. Thus, the peptide was designated frog C-type natriuretic peptide (frog CNP). Frog CNP may participate in the central control of body fluid homeostasis, since its tissue concentration is high in brain.
Yoshihara
A
A
Department of Biochemistry, Miyazaki Medical College, Japan.
Kozawa
H
H
Minamino
N
N
Kangawa
K
K
Matsuo
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001001:Anura; D009320:Atrial Natriuretic Factor / Q000032:analysis*; D001923:Brain Chemistry; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D009126:Muscle Relaxation / Q000187:drug effects; D020098:Natriuretic Peptide, C-Type; D009419:Nerve Tissue Proteins / Q000032:analysis*; D051381:Rats; D012007:Rectum / Q000378:metabolism
D009419:Nerve Tissue Proteins; D020098:Natriuretic Peptide, C-Type; D009320:Atrial Natriuretic Factor