Multigeneration reproduction study of isomalt in rats.
The sugar replacer isomalt was fed to Wistar rats of both sexes throughout three successive generations at concentrations of 0, 2.5, 5 and 10% in the diet. A group of rats fed a diet containing 10% sucrose served as an additional control group. The initial mating was of 100 rats of each sex in each group. For subsequent matings 20 rats of each sex from each group were used. For each generation two litters were reared until they were at least 3 wk old. Feeding isomalt to rats for three successive generations did not induce any adverse effects on fertility, reproductive performance or development compared with control animals fed diets containing maize starch and sucrose instead of isomalt. The second litter of third-generation rats was given detailed gross and microscopic examinations 4 wk after weaning. A marked treatment-related change was an increase in the relative weight of the caecum (filled and empty), 4 wk after weaning in the second litter of third-generation rats fed 10% isomalt. There was also an increase in the relative weight of the filled caecum in males of the 5% isomalt group. These findings were not accompanied by diarrhoea or histological changes in the caecum. The results of the present study did not demonstrate any deleterious effects on the reproduction, maternal performance or development of rats fed isomalt at dietary levels of up to 10% over three successive generations.
Waalkens-Berendsen
D H
DH
TNO-CIVO Toxicology and Nutrition Institute, Zeist, The Netherlands.
Koëter
H B
HB
Sinkeldam
E J
EJ
D000014:Abnormalities, Drug-Induced; D000818:Animals; D001724:Birth Weight / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D002940:Circadian Rhythm; D004187:Disaccharides / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004435:Eating / Q000187:drug effects; D005260:Female; D005298:Fertility / Q000187:drug effects*; D007743:Labor, Obstetric / Q000187:drug effects; D008098:Litter Size / Q000187:drug effects; D008297:Male; D011247:Pregnancy; D011897:Random Allocation; D051381:Rats; D011919:Rats, Inbred Strains; D012098:Reproduction / Q000187:drug effects*; D012744:Sex Ratio; D013047:Specific Pathogen-Free Organisms; D013402:Sugar Alcohols / Q000633:toxicity*; D013549:Sweetening Agents / Q000633:toxicity*
D004187:Disaccharides; D013402:Sugar Alcohols; D013549:Sweetening Agents; C016640:Palatinit