Immunochemical characterization of interactions between circulating autologous immunoglobulin G and normal human erythrocyte membrane proteins.
Autologous immunoglobulin G present during electrophoresis of human erythrocyte membrane proteins influenced the electrophoretic mobility of some of the proteins. Different types of non-ionic detergents were used for solubilization of the membranes and together with experiments using dimyristoylphosphatidylcholine-derived erythrocyte membrane vesicles this indicated that IgG binds to spectrin, ankyrin, and band 3 protein. The binding was independent on proteolysis and not due to unspecific protein-protein interactions. Immunoblotting experiments also showed binding to polypeptide bands in the spectrin and ankyrin regions and demonstrated the presence of erythrocyte-associated IgG. The reactivity may be due to natural autoantibodies involved in the clearance of cellular debris in vivo. Whether the observations are of relevance for the putative immune-mediated clearance of old erythrocytes from the circulation remains to be established.
Heegaard
N H
NH
Blood Bank, Rigshospitalet, Copenhagen, Denmark.
D000017:ABO Blood-Group System; D001457:Anion Exchange Protein 1, Erythrocyte / Q000276:immunology; D017487:Ankyrins; D001323:Autoantibodies / Q000276:immunology*; D001798:Blood Proteins / Q000276:immunology*; D004134:Dimyristoylphosphatidylcholine; D004910:Erythrocyte Membrane / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007123:Immunoelectrophoresis, Two-Dimensional; D007074:Immunoglobulin G / Q000276:immunology*; D007163:Immunosorbent Techniques; D008565:Membrane Proteins / Q000276:immunology; D017830:Octoxynol; D011092:Polyethylene Glycols; D012204:Rh-Hr Blood-Group System; D012995:Solubility; D013049:Spectrin / Q000276:immunology
D000017:ABO Blood-Group System; D001457:Anion Exchange Protein 1, Erythrocyte; D017487:Ankyrins; D001323:Autoantibodies; D001798:Blood Proteins; D007074:Immunoglobulin G; D008565:Membrane Proteins; D012204:Rh-Hr Blood-Group System; D013049:Spectrin; D011092:Polyethylene Glycols; D017830:Octoxynol; D004134:Dimyristoylphosphatidylcholine