New localizations of VH sequences by in situ hybridization with biotinylated probes.
The chromosomal localization of genes of three VH families (VH 1-3) was performed using in situ hybridization with biotinylated probes. Significantly strong signals were observed on chromosome 14, band 14q32, and on bands 16p11 and 15q11, although less frequently. Signal intensity and frequency were more important on chromosome 14 with all three probes, and on chromosome 16 with the VH2 and VH3 probes, while chromosome 15 was more marked than 16 with the VH1 probe. The localization of VH gene on chromosomes other than 14 suggests that several genes of the VH family had been simultaneously translocated in evolution and that the newly localized VH sequences may be pseudogenes.
Cherif
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INSERM U301, Institut de Génétique Moléculaire, Paris, France.
Berger
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R
D001710:Biotin; D002871:Chromosome Banding; D015342:DNA Probes; D005803:Genes, Immunoglobulin; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008297:Male; D009693:Nucleic Acid Hybridization; D011544:Pseudogenes; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D001710:Biotin