The in vitro and in vivo effects of human growth hormone administration on tumor growth of rats bearing a transplantable rat pituitary tumor (7315b).
The direct effects of human GH and IGF-I on PRL secretion and cell proliferation were studied on PRL secreting rat pituitary tumor 7315b cells in vitro, as well as the effects in vivo of human GH administration on body weight, IGF-I levels and tumor size in rats bearing this transplantable tumor. In the in vitro studies IGF-I levels above 5 nM stimulated PRL release in a dose-dependent manner while GH, in concentrations of 0.23-45 nM, did not affect PRL release. Cell proliferation was stimulated by IGF-I in a dose-dependent manner from 0.5 nM onwards, while GH did not have an effect. The in vivo studies showed that 1 mg GH/rat/day prevented tumor-induced cachexia and normalized the suppressed IGF-I levels without stimulating tumor growth. It is concluded that tumor-induced cachexia can be prevented by exogenous GH administration without an increase in tumor mass, even if a tumor model is used whose cultured tumor cells respond to exposure to IGF-I with a mitotic response.
Binnerts
A
A
Department of Medicine and Clinical Endocrinology, Erasmus University, Rotterdam, The Netherlands.
Uitterlinden
P
P
Hofland
L J
LJ
van Koetsveld
P
P
Lamberts
S W
SW
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002100:Cachexia / Q000209:etiology / Q000517:prevention & control; D002455:Cell Division / Q000187:drug effects; D005260:Female; D013006:Growth Hormone / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D009368:Neoplasm Transplantation; D010911:Pituitary Neoplasms / Q000150:complications / Q000473:pathology*; D051381:Rats; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology
D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone