Local effects of granulomatous inflammation on functional activation of T cells in athymic mice.
We investigated the effect of granulomatous inflammation in skin on lymphocyte maturation in athymic (nu/nu) mice. Hepatic egg granulomas developed in euthymic (nu/+) mice with schistosomiasis were transplanted into skin of nu/nu mice. During skin granuloma development the rate of DNA synthesis and interleukin 2 activity of lymphocytes from lymph nodes, with and without concanavalin A stimulation, showed that the nu/nu cells were activated to levels of untreated nu/+ lymph node cells. Activation of splenic lymphocytes was not detected in the grafted nu/nu mice. Also, immunohistochemical staining demonstrated an increase in cells expressing Thy 1.2, Lyt-1 or L3T4 surface markers in the skin and lymph nodes, but not in spleen. The findings indicate that a granulomatous reaction in nu/nu mouse skin induces local, but not systemic, proliferation and differentiation of lymphocytes, to a low degree compatible with resting nu/+ mice.
Fujioka
A
A
Department of Dermatology, University of California, San Francisco.
Suya
H
H
Pincelli
C
C
Hashimoto
A
A
Fukuyama
K
K
Epstein
W L
WL
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D006099:Granuloma / Q000276:immunology*; D007124:Immunoenzyme Techniques; D007376:Interleukin-2 / Q000378:metabolism; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008819:Mice, Nude; D012555:Schistosomiasis mansoni / Q000276:immunology*; D012876:Skin Diseases, Parasitic / Q000276:immunology*; D016038:Skin Transplantation; D013154:Spleen / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000954:Antigens, Surface; D007376:Interleukin-2