High-performance liquid chromatographic assay and erythrocyte partitioning of fleroxacin, a new fluoroquinolone antibiotic.
A rapid technique for sample preparation and HPLC was developed to study in vitro the erythrocyte binding and plasma protein binding of fleroxacin in the concentration range 0.2-15 micrograms ml-1. The red blood cell partition coefficient of fleroxacin was 1.45 +/- 0.18 at 25 degrees C and was independent of concentration, incubation time and temperature. The in vitro plasma protein binding of fleroxacin in humans, determined by two independent methods, was 47-51%. Partition studies using erythrocytes as a simple in vitro model may be useful to predict the degree of tissue uptake and the volume of distribution.
Brunt
E
E
College of Pharmacy, Department of Pharmaceutics, J. Hillis Miller Health Center, University of Florida, Gainesville 32610.
Limberg
J
J
Derendorf
H
H
D000890:Anti-Infective Agents / Q000097:blood*; D001798:Blood Proteins / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002939:Ciprofloxacin / Q000031:analogs & derivatives* / Q000097:blood; D004355:Drug Stability; D004912:Erythrocytes / Q000378:metabolism*; D016576:Fleroxacin; D006801:Humans; D011485:Protein Binding
D000890:Anti-Infective Agents; D001798:Blood Proteins; D002939:Ciprofloxacin; D016576:Fleroxacin