Enzymatic formation of a nonreducing L-ascorbic acid alpha-glucoside: purification and properties of alpha-glucosidases catalyzing site-specific transglucosylation from rat small intestine.
We have previously found that some mammalian tissue homogenates can catalyze a unique transglucosylation from maltose to L-ascorbic acid (AA), resulting in a chemically stable AA derivative, L-ascorbic acid alpha-glucoside (AAG). In the present study, the enzyme responsible for this transglucosylation was isolated from rat intestinal membrane. The formation of AAG was determined by HPLC with an ODS column. The specific activity of AAG-forming enzyme was increased in parallel with that of alpha-glucosidase (maltose hydrolase) during the purification, and two neutral alpha-glucosidases, termed alpha-glucosidases I and II, were purified to apparent homogeneity. Their enzymological properties showed that they corresponded to maltase [EC 3.2.1.20] and sucrase-isomaltase complex [EC 3.2.1.48/10], respectively. Both enzymes could form AAG by splitting only maltose among the disaccharides examined, although alpha-glucosidase I possessed a considerably higher activity than the other enzyme. Both AAG formation and maltose hydrolysis were dependent on incubation temperature with the maximal activity at 60 degrees C, but there was an apparent difference between their pH optima. AAG thus formed could also be hydrolyzed by the purified enzymes. From these results, it is concluded that membrane-bound neutral alpha-glucosidases from rat intestine have site-specific transglucosylase activity to form nonreducing AAG which is distinct from L-ascorbic acid-6-O-alpha-D-glucoside.
Muto
N
N
Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University.
Nakamura
T
T
Yamamoto
I
I
D000818:Animals; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism*; D004187:Disaccharides / Q000378:metabolism; D005960:Glucosides / Q000096:biosynthesis*; D005964:Glucosyltransferases / Q000378:metabolism; D006027:Glycosides / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007421:Intestine, Small / Q000201:enzymology*; D008297:Male; D008320:Maltose / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity; D013696:Temperature; D000520:alpha-Glucosidases / Q000302:isolation & purification*
D004187:Disaccharides; D005960:Glucosides; D006027:Glycosides; D008320:Maltose; D005964:Glucosyltransferases; D000520:alpha-Glucosidases; D001205:Ascorbic Acid