The specific incorporation of labelled aromatic amino acids into proteins through growth of bacteria in the presence of glyphosate. Application to fluorotryptophan labelling to the H(+)-ATPase of Escherichia coli and NMR studies.
Growth of Escherichia coli in the presence of glyphosate, an inhibitor of aromatic amino acid biosynthesis, has permitted the production of proton translocating ATPase that is specifically labelled with 5-fluorotryptophan. Five sets of 19F nuclear magnetic resonances are resolved. The use of glyphosate should be of wide applicability in the preparation of proteins labelled in aromatic amino acid residues for NMR studies.
Kim
H W
HW
Department of Biochemistry, University of Oxford, UK.
Perez
J A
JA
Ferguson
S J
SJ
Campbell
I D
ID
D000596:Amino Acids / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D005456:Fluorescent Dyes; D005998:Glycine / Q000031:analogs & derivatives* / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D006180:Proton-Translocating ATPases / Q000096:biosynthesis*; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D000097797:Glyphosate
D000596:Amino Acids; D005456:Fluorescent Dyes; D005998:Glycine; D006180:Proton-Translocating ATPases; D014364:Tryptophan; D000097797:Glyphosate; C026927:5-fluorotryptophan