Inhibition of osteoclast proton transport by bafilomycin A1 abolishes bone resorption.
Osteoclasts are the main bone resorbing cells with capacity to acidify their intimate contact area with bone. Recent studies have suggested that osteoclast acid secretion is carried out by an H(+)-ATPase. We demonstrate here, that specific inhibitor of vacuolar type H(+)-ATPases, bafilomycin A1, inhibits bone resorption in osteoclast cultures as well as blocks proton transport in isolated medullary bone derived microsomes containing a vacuolar type H(+)-ATPase. These results demonstrate an important role of vacuolar H(+)-ATPase in bone resorption.
Sundquist
K
K
Department of Anatomy, University of Oulu, Finland.
Lakkakorpi
P
P
Wallmark
B
B
Väänänen
K
K
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001693:Biological Transport, Active; D001862:Bone Resorption; D002645:Chickens; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D018942:Macrolides; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D010010:Osteoclasts / Q000378:metabolism*; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats
D000900:Anti-Bacterial Agents; D018942:Macrolides; C040929:bafilomycin A1; D006180:Proton-Translocating ATPases