title: Ultraviolet-induced suppressor T cells and factor(s) in murine contact photosensitivity. III. Mode of action of T-cell-suppressor factor(s) and interaction with cytokines.
abstract: The mode of action of T-cell-suppressor factor (TsF) induced by ultraviolet B (UVB) preirradiation in terms of interaction with several cytokines was studied. Suppression of murine contact photosensitivity (CPS) to 3,3',4',5-tetrachlorosalicylanilide (TCSA) by preirradiation of the sensitizing site to low doses of UVB was caused by antigen-specific suppressor T cells (Ts) and was not associated with the generation of efferent limb-acting suppressor cells. TsF released by Ts inhibited the proliferation of immune lymph node (LN) cells in vitro and reduced interleukin (IL)-2 production of these cells in an antigen-specific fashion without affecting the IL-2 receptor (IL-2R) expression. Both rIL-2 and rGM-CSF have the ability to restore CPS responses in the UVB-preirradiated mice when administered after but not before photosensitization. However, rIL-2 but not rGM-CSF counteracted the in vivo inhibitory effect of TsF. rGM-CSF did not affect the density of I-A+ epidermal Langerhans cells (LCs). It was suggested that TsF inhibited IL-2-mediated immune T-cell proliferation, while rGM-CSF reconstituted the CPS by enhancing the function of photodamaged LCs. These results indicate multiple steps of the UVB-induced immunosuppression circuit, each of which seems to be controlled by different immunomodulators.
authors: Department of Dermatology, School of Medicine, Tokyo Medical and Dental University, Japan.
authors: T
authors: T
authors: Satoh
authors: Y
authors: Y
authors: Tokura
authors: Y
authors: Y
authors: Satoh
authors: M
authors: M
authors: Takigawa
mesh_terms: D000818:Animals; D002455:Cell Division; D016207:Cytokines / Q000502:physiology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000502:physiology; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000096:biosynthesis / Q000502:physiology; D007801:Langerhans Cells / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010787:Photosensitivity Disorders / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis; D011994:Recombinant Proteins; D013491:Suppressor Factors, Immunologic / Q000502:physiology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D016207:Cytokines; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins; D013491:Suppressor Factors, Immunologic; D016178:Granulocyte-Macrophage Colony-Stimulating Factor