Detection of Listeria monocytogenes by using the polymerase chain reaction.
A method was developed for detection of Listeria monocytogenes by polymerase chain reaction amplification followed by agarose gel electrophoresis or dot blot analysis with a 32P-labeled internal probe. The technique identified 95 of 95 L. monocytogenes strains, 0 of 12 Listeria strains of other species, and 0 of 12 non-Listeria strains.
Bessesen
M T
MT
Medical Service, Department of Veterans Affairs Medical Center, Denver, Colorado.
Luo
Q A
QA
Rotbart
H A
HA
Blaser
M J
MJ
Ellison
R T
RT
D001419:Bacteria / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D006801:Humans; D008087:Listeria / Q000302:isolation & purification; D008089:Listeria monocytogenes / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D013045:Species Specificity