title: Production of specific anti-rat 5-HT1A receptor antibodies in rabbits injected with a synthetic peptide.
abstract: Polyclonal antibodies were raised by the repeated injection of rabbits with a synthetic peptide corresponding to a highly selective portion (amino acid residues 243 to 268) of the amino acid sequence of the rat 5-HT1A receptor. The anti-peptide antiserum allowed the immunoprecipitation of 5-HT1A receptors but not of other 5-HT1 sites solubilized from rat hippocampal membranes. Immunoautoradiographic labelling of rat brain sections with the anti-peptide antiserum was superimposed with the autoradiographic distribution of 5-HT1A sites labelled by the selective radioligand [3H]8-OH-DPAT.
authors: INSERM U288, Neurobiologie Cellulaire et Fonctionnelle, Faculté de Médecine Pitié-Salpêtrière, Paris, France.
authors: S
authors: S
authors: el Mestikawy
authors: M
authors: M
authors: Riad
authors: A M
authors: AM
authors: Laporte
authors: D
authors: D
authors: Vergé
authors: G
authors: G
authors: Daval
authors: H
authors: H
authors: Gozlan
authors: M
authors: M
authors: Hamon
mesh_terms: D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D001345:Autoradiography; D007267:Injections; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology*; D011233:Precipitin Tests; D011817:Rabbits / Q000276:immunology; D051381:Rats / Q000276:immunology; D011919:Rats, Inbred Strains; D011985:Receptors, Serotonin / Q000276:immunology*; D013764:Tetrahydronaphthalenes / Q000378:metabolism; D014316:Tritium
chemical_list: D010446:Peptide Fragments; D011985:Receptors, Serotonin; D013764:Tetrahydronaphthalenes; D014316:Tritium; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin