Measurement of biologically active interleukin-1 by a soluble receptor binding assay.
A soluble receptor binding assay has been developed for measuring human interleukin-1 alpha (IL-1 alpha), human IL-1 beta, and mouse IL-1 alpha. The assay is based on a competition between unlabeled IL-1 and 125I-labeled mouse recombinant IL-1 alpha for binding to soluble IL-1 receptor prepared from mouse EL-4 cells. The assay measures only biologically active IL-1 folded in its native conformation. The ratio of human IL-1 alpha to human IL-1 beta can be measured in the same sample by a pretreatment step which removes human IL-1 beta from samples prior to assay. This technique has been used to monitor the purification of recombinant IL-1, and may be utilized to specifically and accurately measure bioactive IL-1 in human serum and cell culture supernatants.
Riske
F
F
Department of Molecular Genetics, Roche Research Center, Hoffmann-La Roche Inc., Nutley, New Jersey.
Chizzonite
R
R
Nunes
P
P
Stern
A S
AS
D000818:Animals; D000906:Antibodies / Q000276:immunology; D001667:Binding, Competitive; D006801:Humans; D007375:Interleukin-1 / Q000276:immunology / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008722:Methods; D051379:Mice; D011971:Receptors, Immunologic / Q000378:metabolism; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins / Q000378:metabolism
D000906:Antibodies; D007375:Interleukin-1; D007457:Iodine Radioisotopes; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins