Modulation of glucose metabolism by sulfonylureas in primary cultures of adult rat hepatocytes.
Addition of tolbutamide (0.1-5 microM) or glipizide (0.05-5 microM) to primary cultures of adult rat hepatocytes caused a dose-dependent increase of fructose 2,6-bisphosphate concentration. This effect was accompanied by a stimulation of the rate of L-lactate production and by an acceleration of the metabolic flux through the reaction catalysed by 6-phosphofructo 1-kinase. Moreover, the continuous presence of tolbutamide during the first 26 hours of culture mimicked long-term insulin effects by raising fructose 2,6-bisphosphate levels and the rate of L-lactate formation. Glucokinase, 6-phosphofructo 1-kinase and total 6-phosphofructo 2-kinase activities were not found to be significantly different in hepatocytes cultured either in the presence or in the absence of sulfonylurea.
López-Alarcón
L
L
Servicio de Bioquímica Experimental, Hospital Puerta de Hierro, Universidad Autónoma de Madrid, Spain.
Melián
E
E
Berbil-Bautista
P R
PR
Guijarro
C
C
Felíu
J E
JE
D000818:Animals; D002478:Cells, Cultured; D005635:Fructosediphosphates / Q000378:metabolism; D005913:Glipizide / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology; D007773:Lactates / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010732:Phosphofructokinase-1 / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014044:Tolbutamide / Q000494:pharmacology*
D005635:Fructosediphosphates; D007328:Insulin; D007773:Lactates; C027652:fructose 2,6-diphosphate; D014044:Tolbutamide; D010732:Phosphofructokinase-1; D005947:Glucose; D005913:Glipizide