[Synthesis of new fluorogenic substrates derivated of 7-amino-4-trifluoromethylcoumarin. Detection of gram-negative bacteria, Streptococci group A and Enterococci].
The synthesis of substrates having 7-amino 4-trifluoromethyl coumarin as fluorogenic reagent is described. The first group is performed from L-alanine derivative in order to investigate L-alanine aminopeptidase activity for the detection of Gram-negative bacteria. The second group is synthesized from the L-pyrrolidon carboxylic acid. It allows us to detect the PYRase activity of group A Streptococci and Enterococci. These substrates are evaluated with the N-protected form and the amino free one.
Agban
A
A
Laboratoire de Chimie-Pharmacie, URA CNRS n degrees 1287, Université Joseph-Fourier, Grenoble I, UFR de Pharmacie, La Tronche.
Ounanian
M
M
Luu-Duc
C
C
Monget
D
D
D000626:Aminopeptidases / Q000032:analysis; D003374:Coumarins / Q000138:chemical synthesis*; D004755:Enterobacteriaceae / Q000201:enzymology; D006090:Gram-Negative Bacteria / Q000201:enzymology; D013297:Streptococcus pyogenes / Q000201:enzymology
D003374:Coumarins; C055467:7-amino-4-trifluoromethylcoumarin; D000626:Aminopeptidases
Synthèse de nouveaux substrats fluorogéniques dérivés de la 7-amino 4-trifluorométhyl coumarine. Détection des bactéries Gram négatif, des Streptocoques groupe A et des Entérocoques.