title: Functional expression in vitro of bovine visual rhodopsin.
abstract: Expression of functionally active bovine visual rhodopsin was achieved by sequential transcription and translation in vitro of rhodopsin gene cDNA with co-translational insertion of the protein into phosphatidylcholine liposomes. The recombinant rhodopsin has functional, spectral and immunochemical properties similar to those of natural rhodopsin from bovine retina. Two mutant rhodopsins, Cys316----Ser and Asp330----Asn, Asp331----Asn, were produced by oligonucleotide-directed mutagenesis. The first mutation does not affect rhodopsin's ability to stimulate transducin GTPase and visual cGMP phosphodiesterase activities, while the second double mutation leads to a sharp decrease in rhodopsin activity.
authors: Branch of M.M.Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences, Pushchino, Moscow Region, USSR.
authors: S A
authors: SA
authors: Zozulya
authors: V V
authors: VV
authors: Gurevich
authors: T A
authors: TA
authors: Zvyaga
authors: E P
authors: EP
authors: Shirokova
authors: I L
authors: IL
authors: Dumler
authors: M N
authors: MN
authors: Garnovskaya
authors: Natochin MYu
authors: B E
authors: BE
authors: Shmukler
authors: P R
authors: PR
authors: Badalov
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005136:Eye Proteins / Q000235:genetics; D015870:Gene Expression; D005796:Genes; D008969:Molecular Sequence Data; D009154:Mutation; D014176:Protein Biosynthesis; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D012168:Retinal Pigments / Q000235:genetics*; D012243:Rhodopsin / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D017299:Rod Opsins; D014158:Transcription, Genetic
chemical_list: D005136:Eye Proteins; D011994:Recombinant Proteins; D012168:Retinal Pigments; D017299:Rod Opsins; D004247:DNA; D012243:Rhodopsin