title: Vascular endothelial differentiation in sponge matrix allografts.
abstract: These studies test the hypothesis that vascular endothelia in sponge allografts may develop a function and phenotype resembling the high endothelial venules (HEV) in lymph nodes, thus facilitating the lymphocytic infiltration that is characteristic of allograft rejection. Using limiting dilution analysis to quantitate helper-T-cell accumulation at graft sites, immunohistologic analysis of graft reactivity with the HEV-specific monoclonal antibody MECA 325, and ex vivo lymphocyte-endothelial adhesion assays with graft tissues, we obtained evidence to suggest that HEV-like endothelia may develop at a graft site but that the process whereby lymphocytes accumulate at a graft site is more complex than was initially expected.
authors: University of Utah School of Medicine, Division of Hematology/Oncology, Salt Lake City 84132.
authors: D K
authors: DK
authors: Bishop
authors: D D
authors: DD
authors: Sedmak
authors: D M
authors: DM
authors: Leppink
authors: C G
authors: CG
authors: Orosz
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002454:Cell Differentiation; D004729:Endothelium, Lymphatic / Q000276:immunology; D004730:Endothelium, Vascular / Q000276:immunology*; D005260:Female; D006084:Graft Rejection / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D014184:Transplantation, Homologous / Q000276:immunology; D014185:Transplantation, Isogeneic / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal