ATP binding at noncatalytic sites of soluble chloroplast F1-ATPase is required for expression of the enzyme activity.
The F1-ATPase from chloroplasts (CF1) lacks catalytic capacity for ATP hydrolysis if ATP is not bound at noncatalytic sites. CF1 heat activated in the presence of ADP, with less than one ADP and no ATP at non-catalytic sites, shows a pronounced lag in the onset of ATP hydrolysis after exposure to 5-20 microM ATP. The onset of activity correlates well with the binding of ATP at the last two of the three noncatalytic sites. The dependence of activity on the presence of ATP at non-catalytic sites is shown at relatively low or high free Mg2+ concentrations, with or without bicarbonate as an activating anion, and when the binding of ATP at noncatalytic sites is slowed 3-4-fold by sulfate. The latent CF1 activated by dithiothreitol also requires ATP at noncatalytic sites for ATPase activity. A similar requirement by other F1-ATPases and by ATP synthases seems plausible.
Milgrom
Y M
YM
Department of Chemistry and Biochemistry and Molecular Biology Institute, University of California, Los Angeles 90024.
Ehler
L L
LL
Boyer
P D
PD
D000255:Adenosine Triphosphate / Q000378:metabolism*; D001665:Binding Sites; D002736:Chloroplasts / Q000201:enzymology*; D006868:Hydrolysis; D007700:Kinetics; D046911:Macromolecular Substances; D008274:Magnesium / Q000494:pharmacology; D010944:Plants / Q000201:enzymology; D006180:Proton-Translocating ATPases / Q000378:metabolism*
D046911:Macromolecular Substances; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases; D008274:Magnesium