Immunoreactive atrial natriuretic factor in aqueous humor: its concentration is increased with high intraocular pressure in rabbit eyes.
Atrial natriuretic factor (ANF) concentration in the aqueous humor (AH) was studied in rabbits with experimental glaucoma induced by injecting alpha-chymotrypsin into the posterior chamber. In normal rabbit eyes, the ANF concentration in AH was 3.1 +/- 1.2 pg/ml (mean +/- SEM; n = 12), ranging from 0 to 5.8 pg/ml, whereas it was significantly higher in AH from glaucomatous rabbit eyes, being 81.0 +/- 9.8 pg/ml (n = 12). These findings were correlated with intraocular pressure (IOP), which was 13.0 +/- 2.4 mmHg (n = 12) in normal rabbit eyes and significantly greater in glaucomatous eyes: 24.4 +/- 3.0 mmHg (n = 12). Our data indicate that enhanced ANF release in AH during experimental glaucoma may play an important physiological role in modulating IOP.
Fernandez-Durango
R
R
Diabetic, Hypertension and Obesity Unit, Hospital Universitario San Carlos, Ciudad Universitaria, Madrid, Spain.
Triviño
A
A
Ramirez
J M
JM
Garcia de Lacoba
M
M
Ramirez
A
A
Salazar
J J
JJ
Fernandez-Cruz
A
A
Gutkowska
J
J
D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D002918:Chymotrypsin; D005123:Eye / Q000473:pathology; D005901:Glaucoma / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D008297:Male; D011817:Rabbits
D009320:Atrial Natriuretic Factor; D002918:Chymotrypsin