Production of colony-stimulating factor-1 (CSF-1) by mouse astroglia in vitro.
We investigated whether astroglia produce any of the known macrophage growth factors, CSF-1, GM-CSF, and IL-3, and if so, whether any of these cytokines stimulate the growth of CNS macrophages. In this work we used highly enriched cell cultures of C3H/HeJ mouse neopallium: cultures of astroglia and cultures of macrophage-like cells derived from nutritionally deprived astroglia cultures. We found that astroglia in cultures accumulate in the medium, an activity that stimulates the proliferation of macrophage-like cells. The activity has been identified as CSF-1 by using growth assays of cells dependent and nondependent on CSF-1, and by radioreceptor analysis which is highly specific for CSF-1. Northern blot analysis demonstrated the presence in astroglia of CSF-1 mRNA and the presence of CSF-1 receptor (c-fms) mRNA in macrophage-like cells but not in astroglia. The astroglia did not produce GM-CSF or IL-3. We concluded that a paracrine relationship exists between astroglia production of CSF-1 and the response of macrophage-like cells to the cytokine in culture.
Hao
C
C
Department of Anatomy, University of Saskatchewan, Saskatoon, Canada.
Guilbert
L J
LJ
Fedoroff
S
S
D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016173:Macrophage Colony-Stimulating Factor / Q000096:biosynthesis* / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D012333:RNA, Messenger / Q000096:biosynthesis; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000096:biosynthesis / Q000378:metabolism; D013268:Stimulation, Chemical; D014407:Tumor Cells, Cultured / Q000187:drug effects
D012333:RNA, Messenger; D016173:Macrophage Colony-Stimulating Factor; D016186:Receptor, Macrophage Colony-Stimulating Factor