Corticosteroids increase lipocortin I in alveolar epithelial cells.
Lipocortin I mediates, in part, the anti-inflammatory effects of corticosteroids. To determine whether alveolar epithelial cells produce lipocortin I, we used a highly specific anti-lipocortin I antibody and found by the techniques of Western blotting and immunocytochemical analysis that lipocortin I is a normal constituent of type II alveolar epithelial cells and that it constitutes approximately 0.1% of cell lysate proteins. Two separate species of lipocortin I were identified, migrating with molecular weights of 37 and 32 kD. Dexamethasone caused a dose-dependent increase in lipocortin I in rat alveolar epithelial cells, and its effect was maximal at a dose of 10(-5) M. This effect was specific for corticosteroids since it was not seen with other steroids. These studies suggest that lipocortin I may mediate, in part, the immunosuppressive effects of corticosteroids in the lower respiratory tract.
Ambrose
M P
MP
Department of Medicine, Department of Veterans Affairs, Iowa City, Iowa.
Hunninghake
G W
GW
D000818:Animals; D017302:Annexins; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004848:Epithelium / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology; D007124:Immunoenzyme Techniques; D008970:Molecular Weight; D010449:Peptide Mapping; D011374:Progesterone / Q000494:pharmacology; D011650:Pulmonary Alveoli / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000494:pharmacology
D017302:Annexins; D002135:Calcium-Binding Proteins; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D003907:Dexamethasone