title: Soybean trypsin inhibitor and beta-amylase stimulate macrophages.
abstract: Mature soybean seeds were found to contain macrophage stimulation activity. The activity existed in a water-soluble fraction of soybean whey. Gel filtration, DEAE-cellulose column chromatography and high-performance liquid chromatography of the water-soluble fraction gave two active proteins. By N-terminal and total amino acid analyses, these were effectively stimulated by each of the two proteins to produce nitrite.
authors: National Institute of Animal Industry, Tsukuba Norindanchi, Ibaraki, Japan.
authors: M
authors: M
authors: Miwa
authors: Z L
authors: ZL
authors: Kong
authors: K
authors: K
authors: Shinohara
authors: M
authors: M
authors: Watanabe
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002460:Cell Line; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D009573:Nitrites / Q000378:metabolism; D013025:Glycine max; D014361:Trypsin Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D001614:beta-Amylase / Q000494:pharmacology*
chemical_list: D000596:Amino Acids; D009573:Nitrites; D014361:Trypsin Inhibitors; D001614:beta-Amylase