An unusual form of lipid linkage to the CD45 peptide.
Some protein kinases and phosphatases are myristoylated on their amino terminus, which perhaps contributes to subcellular localization or regulation. Glycoprotein CD45, a hematopoietic tyrosine phosphatase, was examined for fatty acid content. The CD45 protein incorporated [3H]myristate, but little [3H]palmitate. The label was not metabolized and reincorporated into amino acids or saccharides, as revealed by peptide maps of CD45 labeled with [3H]myristate, 14C-labeled amino acids, [35S]methionine, or 125I, and glycosidase treatments, respectively. The myristate label was resistant to mild alkaline methanolysis and was found in fatty acid and sphingosine, indicating an unusual form of lipid attachment to CD45.
Takeda
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Department of Pathology, Roger Williams General Hospital, Brown University, Providence, RI 02908.
Maizel
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D000943:Antigens, Differentiation / Q000032:analysis* / Q000378:metabolism; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000032:analysis*; D006649:Histocompatibility Antigens / Q000032:analysis* / Q000378:metabolism; D017493:Leukocyte Common Antigens; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D010449:Peptide Mapping; D013107:Sphingolipids / Q000378:metabolism
D000943:Antigens, Differentiation; D005227:Fatty Acids; D006649:Histocompatibility Antigens; D009227:Myristic Acids; D010169:Palmitic Acids; D013107:Sphingolipids; D019814:Myristic Acid; D019308:Palmitic Acid; D017493:Leukocyte Common Antigens