Fragment pathway analysis using automated tandem mass spectrometry on an ion-trap mass spectrometer.
A "key-sequence" procedure is presented for the automated tandem mass spectrometric analysis of compounds on a Finnigan ion-trap mass spectrometer. This allows fragmentation pathways of a range of masses or even a complete spectrum to be prepared automatically, obviating the tiresome preparation and optimization of individual scan-editor files. The procedure is limited by the speed of the driving computer; an "IBM-AT", for example, permits more than 10 mass units to be scanned per minute. It is calibrated with perfluorotributylamine and methyl stearate is used to demonstrate its results. The Finnigan ion-trap "programming option" is necessary for implementation of the procedure.
Pannell
L K
LK
Laboratory of Bioorganic Chemistry, NIDDK, NIH, Bethesda, MD 20892.
Pu
Q L
QL
Mason
R T
RT
Fales
H M
HM
D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*