Uptake and incorporation of arachidonic acid and of dihomogamma-linolenic acid into tissue lipids in uterine strips isolated from ovariectomized and from ovariectomized-diabetic rats. Effects of 17-beta-estradiol.
The uptake of 14C-arachidonic acid (14C-AA) and 14C-dihomogamma-linolenic acid (14C-DGLA) into phospholipids (PLs) and neutral lipids (NLs) in uterine tissue obtained from ovariectomized controls (C) and from ovariectomized-diabetic rats (D) was studied. Uterine strips from D rats incorporate significantly less (P less than 0.05) 14C-AA into PLs than C rats. On the other hand the uptake of 14C-AA into NLs is significantly smaller (P less than 0.05) in uterine tissue from C than from D animals. The estrogenization of the C animals did not modify the incorporation of 14C-AA into PLs or NLs. On the contrary, uterine tissue obtained from D rats treated with 17-beta-estradiol incorporated significantly more labelled AA (P less than 0.05) into PLs and significantly less 14C-AA (P less than 0.05) into NLs than untreated D animals. The incorporation of 14C-DGLA into PLs shows similar pattern in uterine tissue obtained from C and D animals. Estrogenization increased significantly (P less than 0.01) in both cases, the incorporation into PLs. Regarding the incorporation of 14C-DGLA, it was clearly shown that DGLA is taken up significantly more (P less than 0.01) by NLs than by PLs, both in C and D rats. The estrogenization of C and D rats induces a significant decrease in the incorporation of 14C-DGLA into NLs in both experimental groups. The distribution of 14C-AA into the different subfractions of PLs is not uniform.(ABSTRACT TRUNCATED AT 250 WORDS)
Chaud
M
M
Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina (CONICET), Buenos Aires.
Franchi
A M
AM
Gonzalez
E T
ET
Gimeno
A L
AL
Gimeno
M A
MA
D015126:8,11,14-Eicosatrienoic Acid / Q000378:metabolism / Q000493:pharmacokinetics*; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism / Q000493:pharmacokinetics*; D001693:Biological Transport, Active / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D010052:Ovariectomy; D051381:Rats; D011919:Rats, Inbred Strains; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
D001095:Arachidonic Acids; D016718:Arachidonic Acid; D004958:Estradiol; D015126:8,11,14-Eicosatrienoic Acid