Fast induction of translatable mRNA by blue light in Neurospora crassa wt: the wc-1 and wc-2 mutants are blind.
After blue-light irradiation of Neurospora crassa (wt) mycelia we observed an increase of about 13 translatable mRNA species within a period of 30 min. The induction of translatable mRNA species followed a specific temporal pattern which permitted the identification of four distinct classes. One of the translatable mRNAs was induced in less than 2 min, while the others showed lag periods of 5, 10 or 20 min from the beginning of illumination. The white collar mutants, wc-1 and wc-2, which do not display any of the blue-light-induced physiological effects tested until now were found to be defective for the photoinduction of translatable mRNAs.
Nawrath
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Max-Planck-Institut für molekulare Genetik, Berlin, F.R.G.
Russo
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VE
D015180:Electrophoresis, Gel, Two-Dimensional; D008027:Light; D009154:Mutation; D009491:Neurospora / Q000528:radiation effects*; D009492:Neurospora crassa / Q000235:genetics / Q000528:radiation effects*; D014176:Protein Biosynthesis / Q000528:radiation effects*; D012331:RNA, Fungal / Q000096:biosynthesis / Q000528:radiation effects*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000528:radiation effects*; D013997:Time Factors
D012331:RNA, Fungal; D012333:RNA, Messenger