Incorporation of SP-40,40 into the soluble membrane attack complex (SMAC, SC5b-9) of complement.
When SC5b-7 was prepared from the C8-depleted serum activated with inulin, it contained SP-40,40 as well as S-protein. From the densitometry of each component in SC5b-9 after SDS-PAGE, it was estimated that SC5b-9 was constituted of one molecule each of C5b, C6, C7, C8, S-protein, and SP-40,40 and two molecules of C9. SP-40,40 was depleted from normal serum with an affinity column using mouse monoclonal anti-SP-40,40 antibody. When the resulting SP-40,40-depleted serum was activated with inulin, SC5b-9 lacking SP-40,40 could be formed. S-Protein-depleted serum was also prepared with an affinity column using mouse monoclonal anti-S-protein antibody. Similarly, SC5b-9 lacking S-protein could be formed by the inulin activation of the S-protein-depleted serum. These results indicate that either SP-40,40 or S-protein should be able to form a soluble C5b-9 complex.
Choi
N H
NH
Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Nakano
Y
Y
Tobe
T
T
Mazda
T
T
Tomita
M
M
D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism*; D015153:Blotting, Western; D051152:Clusterin; D015938:Complement Membrane Attack Complex / Q000737:chemistry / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D005779:Immunodiffusion; D066298:In Vitro Techniques; D018832:Molecular Chaperones; D017293:Protein S; D012995:Solubility
D001798:Blood Proteins; C495242:CLU protein, human; D051152:Clusterin; D015938:Complement Membrane Attack Complex; D006023:Glycoproteins; D018832:Molecular Chaperones; D017293:Protein S