title: Reversible inactivation of water and sodium transport systems in frog urinary bladder and skin in the presence of detergents.
abstract: Detergents (Triton X-100, dodecyl sulphate, saponin) added in concentrations of 0.1-10 mg/ml to solution at the outer frog skin surface reversibly reduced potential difference (PD) and short-circuit current (Isc); when added at the inner surface. Triton X-100 initially induced a short increase in the PD and Isc followed by a pronounced decrease similarly as after the application of any of the detergents used. When added to either serosal or mucosal surface of frog urinary bladder, the detergents reversibly abolished the reactivity to vasopressin. Triton X-100 blocked foskolin and cAMP-induced effects on membrane water permeability. The results suggest that hydrophobic elements of membrane play a crucial role in the regulation of membrane permeability for ions and water and of the responsiveness of the cells to vasopressin. Differences in sensitivity to detergents could be observed between the apical and the basolateral cell membrane.
authors: Institute of Evolutionary Physiology and Biochemistry, Academy of Sciences of the USSR, Leningrad.
authors: Natochin YuV
authors: E I
authors: EI
authors: Shakhmatova
authors: P
authors: P
authors: Bakos
mesh_terms: D000818:Animals; D001692:Biological Transport; D002463:Cell Membrane Permeability; D003902:Detergents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D017830:Octoxynol; D009995:Osmosis / Q000187:drug effects; D011092:Polyethylene Glycols / Q000494:pharmacology; D011188:Potassium / Q000493:pharmacokinetics; D015221:Potassium Channels / Q000187:drug effects; D011896:Rana temporaria; D012503:Saponins / Q000494:pharmacology; D012867:Skin / Q000378:metabolism; D012964:Sodium / Q000493:pharmacokinetics*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology*; D001743:Urinary Bladder / Q000187:drug effects* / Q000378:metabolism; D014867:Water / Q000378:metabolism*
chemical_list: D003902:Detergents; D015221:Potassium Channels; D012503:Saponins; D013501:Surface-Active Agents; D014867:Water; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; D017830:Octoxynol; D012964:Sodium; C028913:dodecyl sulfate; D011188:Potassium