Correlation between fibronectin and its receptor in chick myoblast differentiation.
Alterations in the amount of fibronectin and in the number of its receptors during myoblast differentiation of chicken embryo were investigated. The amount of fibronectin in the cell surface pool as measured by immunoblotting decreased during myogenesis. To identify and characterize the fibronectin receptors on the myoblasts, the interactions of the 28,000 dalton (28 kDa) amino terminal fragment and 85,000 dalton (85 kDa) cell-binding fragment of fibronectin with myoblasts were examined. The binding of the 28 kDa fragment was found to be time-dependent and reached a maximum level within 60 min. The unlabeled 28 kDa fragment inhibited the binding of the radioiodinated 28 kDa fragment, whereas the unlabeled 85 kDa fragment and antibody to integrin did not inhibit it, suggesting that the 28 kDa fragment interacts with the matrix assembly receptors but not with the cell adhesion receptors. There was a single class of 3.4 x 10(5) binding sites per cell with an apparent dissociation constant of 1.4 x 10(-7) M on 30 hr old myoblasts. The specific binding of the radioiodinated 28 kDa fragment to myoblasts decreased as the fusion proceeded. This decrease of binding was consistent with the decrease in the amount of fibronectin. Furthermore, the levels of fibronectin and binding of the radioiodinated 28 kDa fragment in the fusion-blocked myoblasts by EGTA treatment appeared to remain constant. These results suggest that the decrease and/or loss of fibronectin during myoblast fusion is closely correlated with the alteration of fibronectin receptors and with the fusion of myoblasts.
Chung
C Y
CY
Department of Zoology, College of Natural Science, Seoul National University, Korea.
Kang
M S
MS
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D004533:Egtazic Acid / Q000494:pharmacology; D005353:Fibronectins / Q000378:metabolism*; D015151:Immunoblotting; D007700:Kinetics; D008970:Molecular Weight; D009132:Muscles / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000378:metabolism*; D013820:Thermolysin / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
D005353:Fibronectins; D010446:Peptide Fragments; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic; D004533:Egtazic Acid; D014357:Trypsin; D013820:Thermolysin