title: Steroid sulphatase activity in the human ovarian corpus luteum, stroma, and follicle: comparison to activity in other tissues and the placenta.
abstract: Steroid sulfatase activity was measured in 89 human samples, using dehydroepiandrosterone sulfate (DHEAS) as substrate. The lowest activity was that of follicular fluid which was significantly lower than that of other tissues tested (each P less than 0.01). The steroid sulfatase activity of ovarian tissue taken collectively (corpus luteum, stroma, and follicles) was higher than that of other tissues taken collectively (abdominal skin, uterus, and fallopian tube) (P less than 0.001), and the steroid sulfatase activity of either the follicle (P less than 0.01) or the stroma (P less than 0.05) was significantly greater than that of the corpus luteum. The geometric mean steroid sulfatase activity of the placenta was significantly higher than other tissues tested (each P less than 0.01) and was 22-fold higher than that of the follicle, the tissue with the next highest activity. These data indicate that the human ovary (particularly the stroma and follicle) is capable of utilizing DHEAS, an adrenal product, as a substrate for production of other androgens such as dehydroepiandrosterone (DHEA), androstenedione, and testosterone.
authors: Department of Obstetrics and Gynecology, Brown University, Providence, RI.
authors: R V
authors: RV
authors: Haning
authors: R J
authors: RJ
authors: Hackett
authors: R I
authors: RI
authors: Boothroid
authors: J A
authors: JA
authors: Canick
mesh_terms: D001192:Arylsulfatases / Q000378:metabolism*; D003338:Corpus Luteum / Q000201:enzymology; D003687:Dehydroepiandrosterone / Q000031:analogs & derivatives* / Q000378:metabolism; D019314:Dehydroepiandrosterone Sulfate; D005260:Female; D006801:Humans; D007700:Kinetics; D009928:Organ Specificity; D006080:Ovarian Follicle / Q000201:enzymology; D010053:Ovary / Q000201:enzymology*; D010920:Placenta / Q000201:enzymology*; D043266:Steryl-Sulfatase; D013429:Sulfatases / Q000378:metabolism*
chemical_list: D003687:Dehydroepiandrosterone; D019314:Dehydroepiandrosterone Sulfate; D013429:Sulfatases; D001192:Arylsulfatases; D043266:Steryl-Sulfatase