Decreased lymphocyte blastogenesis, IL2 production and NK activity following nifedipine administration to healthy humans.
The effects of a single oral dose of nifedipine on part of the immune response in healthy humans has been investigated in terms of two different immune functions: T lymphocyte proliferation and NK activity. Both functions are known to require calcium ions. Ten healthy subjects were bled before and 30 min, and 4 and 24 h after receiving 10 mg nifedipine. Lymphocyte proliferation, both in mitogen-activated lymphocyte cultures, and in autologous and allogeneic mixed lymphocyte reactions, was significantly reduced (up to 48%) 30 min after drug administration and reverted to normal 4 h later. The inhibition could be attributed to reduction in IL2 production by the T cells isolated 30 min following the administration of nifedipine, since they normally express IL2-receptors. The addition of recombinant IL2 of 200 U.ml-1 to the cell cultures restored their responsiveness. NK activity was significantly reduced 30 min and 4 h after drug administration and returned to normal at the 24th h. This function was also restored by the addition of IL2. The data suggest that calcium channel blockers may inhibit, at least transiently, lymphocyte functions in vivo.
Morgano
A
A
Department of Internal Medicine, University of Genoa, Italy.
Pierri
I
I
Stagnaro
R
R
Setti
M
M
Puppo
F
F
Indiveri
F
F
D000284:Administration, Oral; D000328:Adult; D002478:Cells, Cultured; D005260:Female; D006680:HLA Antigens / Q000502:physiology; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000378:metabolism; D007694:Killer Cells, Natural / Q000187:drug effects / Q000502:physiology; D008213:Lymphocyte Activation / Q000187:drug effects*; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology; D008297:Male; D008875:Middle Aged; D008934:Mitogens / Q000494:pharmacology; D009543:Nifedipine / Q000008:administration & dosage / Q000494:pharmacology*; D015375:Receptors, Interleukin-2 / Q000502:physiology; D013601:T-Lymphocytes / Q000187:drug effects / Q000502:physiology
D006680:HLA Antigens; D007166:Immunosuppressive Agents; D007376:Interleukin-2; D008934:Mitogens; D015375:Receptors, Interleukin-2; D009543:Nifedipine