Assignment of the human gene for CREB1 to chromosome 2q32.3-q34.
The nuclear phosphoprotein cAMP-response-element-binding-protein 1 (CREB1) is involved in the transcriptional regulation of cAMP-inducible genes. We have determined the chromosomal location of the human CREB1 gene by using a cDNA probe. Southern blot analysis of genomic DNA from a panel of mouse-human somatic cell hybrids indicated that the CREB1 gene resides on human chromosome 2. Analysis of a somatic cell hybrid containing a chromosome 2 translocation narrowed assignment of the CREB1 locus to the terminal third of chromosome 2. Finally, in situ hybridization allowed localization of the gene to the 2q32.3-q34 region. This knowledge may prove valuable in molecular analysis of translocations occurring in this region and in exploring the possibility of a role for the CREB1 locus in malignancy.
Taylor
A K
AK
Department of Medicine, University of California, Los Angeles 90024.
Klisak
I
I
Mohandas
T
T
Sparkes
R S
RS
Li
C
C
Gaynor
R
R
Lusis
A J
AJ
D000818:Animals; D015139:Blotting, Southern; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D017362:Cyclic AMP Response Element-Binding Protein; D004268:DNA-Binding Proteins / Q000235:genetics*; D005796:Genes; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D014178:Translocation, Genetic
D017362:Cyclic AMP Response Element-Binding Protein; D004268:DNA-Binding Proteins