title: Is the intestine the sole source of heparin-stimulated plasma diamine oxidase? Acute effects of jejunectomy, ileectomy and total enterectomy.
abstract: The assumption that the intestine is the sole/major source of post-heparin diamine oxidase (DAO) has been tested only once by Kobayashi et al. in 1969. In this study we tested whether the intestine is indeed the sole/major source of post-heparin plasma DAO and furthermore we studied the relative contribution of jejunum and ileum to post-heparin plasma DAO. We measured non-fasting plasma DAO before and every 15 min for 2 h after i.v. heparin (4,000 U/kg BW) and compared the resultant areas under the concentration-time curves (AUCs: mU/l X 2 h) in unoperated controls, jejunectomised, ileectomised and totally enterectomised (from gastro-oesophageal to colorectal junctions) male Wistar rats. Total enterectomy virtually abolished the post-heparin rise in plasma DAO, the mean AUC falling from 55.3 +/- (SEM) 10.2 in controls to 2.1 +/- 1.1 after enterectomy (p less than 0.001). The mean AUCs after jejunectomy and ileectomy were 25.6 +/- 5.5 and 10.75 +/- 2.42, respectively. Therefore in acute jejunal and ileal resection the AUCs were 2.1 and 5.4 times, respectively, lower than those seen in the controls (p less than 0.02 and p less than 0.001). The difference in mean AUC values between jejunectomy and ileectomy groups was also statistically significant (p less than 0.05). We conclude that total enterectomy abolishes the post-heparin rise in circulating DAO activity, confirming that the intestine is the major source of post-heparin plasma DAO. The ileum contributes more than the jejunum to this post-heparin rise in plasma enzyme activity.
authors: Gastroenterology Unit, UMDS, London, UK.
authors: T
authors: T
authors: Rokkas
authors: S
authors: S
authors: Vaja
authors: P
authors: P
authors: Taylor
authors: G M
authors: GM
authors: Murphy
authors: R H
authors: RH
authors: Dowling
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000097:blood / Q000378:metabolism*; D000818:Animals; D006493:Heparin / Q000494:pharmacology*; D007082:Ileum / Q000201:enzymology / Q000601:surgery*; D007413:Intestinal Mucosa / Q000201:enzymology*; D007583:Jejunum / Q000201:enzymology / Q000601:surgery*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
chemical_list: D006493:Heparin; D006631:Amine Oxidase (Copper-Containing)