[Acid and basic glycoproteins of human saliva. 1. Investigation of glycoproteins of mixed saliva].
By investigating the possibility of the use of elektrophoretic method on the Pol-E agarose film in human mixed saliva, we concluded that these samples should be lyophilised, and the content of proteins of dissolved samples should sorrespond to the concentration which is situated in the normal human serum. The electrophoretic mobility of fractions of mixed saliva in each varied condition (pH = 5,9 pH = 7 pH = 8,6 and pH = 11) of time, of voltage and of different sample volume applicated to the gel. Thet mobility was exclusively anodic. The majority of fractions (5 fractions) xas separated at pH = 11 and a prolonged of electrophoresis. These fractions were acid and of the biggest electrophoretic mobility. According to the place of separation and intensity (in relation to the normal human serum) it was evident that it was question of acid fractions. In mixed saliva characteristic glyco-and phosphoproteins and albumins were registered. This method enables the registration of enumerated fractions, though it is not selective enough for saliva as a biological material.
Mirković
S
S
University School of Dental Medicine, Belgrade.
D004587:Electrophoresis, Agar Gel; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012471:Salivary Proteins and Peptides / Q000032:analysis*
D006023:Glycoproteins; D012471:Salivary Proteins and Peptides
Glikoproteini humane pljuvacke. I. Ispitivanje glikoproteina mesovite pljuvacke.