Ontogenic appearance of MHC class I (B-F) antigens during chicken embryogenesis.
Expression of chicken MHC class I (B-F) antigens during ontogeny was determined by binding of anticlass I antibody and appearance of B-F transcripts by Northern blotting in chicken organs during embryogenesis until 2 weeks after hatching. MHC class I transcripts first become detectable in day 6.5 of embryogenesis. B-F cell-surface expression first becomes detectable in hemopoietic organs by day 10-12 of embryogenesis and somewhat later in nonhemopoietic organs. Flow cytometry analysis of hemopoietic cells throughout embryogenesis revealed B-Fhi and B-Flo cell populations. The percentage of B-F+ cells in spleen and bone marrow decreased around hatching, which could reflect either cell flows in these organs during this period or the sensitivity of hemopoietic cells to hatching stress.
Dunon
D
D
Basel Institute for Immunology, Switzerland.
Salomonsen
J
J
Skjødt
K
K
Kaufman
J
J
Imhof
B A
BA
D000818:Animals; D002642:Chick Embryo / Q000276:immunology*; D015870:Gene Expression; D006413:Hematopoietic System / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014158:Transcription, Genetic
D015395:Histocompatibility Antigens Class I; D012333:RNA, Messenger