A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells.
Leukocyte-endothelial cell (EC) interactions regulate the entry of immune effectors into the tissues. This interaction occurs in lymphoid tissues and inflammatory sites at post-capillary high endothelial venules, as opposed to large capacitance vessels lined with flat EC. Transient SV40 infection of mouse EC derived from lymph node stroma has resulted in a cell line, SVEC4-10, that retains morphological and functional characteristics of normal EC. SVEC4-10 cells grow efficiently on plastic as a monolayer with a characteristic epithelioid morphology. They require as little as 2% FCS and are independent of other exogenous growth factors or matrix components. When grown on a synthetic basement membrane, SVEC4-10 forms branching tube-like networks. SVEC4-10 expresses Factor VIII related Ag as measured by indirect immunofluorescence using a rabbit antiserum to human FVIII-associated protein and incorporates acetylated low density lipoprotein. SVEC4-10 specifically binds mouse lymphocytes in vitro. IFN-gamma induces expression of MHC class II Ag in a time course identical to normal EC and the cell line is susceptible to lysis by anti SV40 H-2k CTL clones. Thus, this SV40 immortalized line retains much of the normal cellular physiology of EC.
O'Connell
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KA
Department of Biology, Johns Hopkins University, Baltimore, MD 21218.
Edidin
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D000818:Animals; D001485:Basement Membrane / Q000502:physiology; D002448:Cell Adhesion; D002460:Cell Line; D002472:Cell Transformation, Viral; D003094:Collagen / Q000502:physiology; D003602:Cytotoxicity, Immunologic; D004727:Endothelium / Q000166:cytology*; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D007797:Laminin / Q000502:physiology; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000166:cytology*; D051379:Mice; D011994:Recombinant Proteins; D013539:Simian virus 40
D000949:Histocompatibility Antigens Class II; D007375:Interleukin-1; D007797:Laminin; D011994:Recombinant Proteins; D007371:Interferon-gamma; D003094:Collagen