VHDJH to JH joining is not blocked in mu-chain-producing pre-B cells, implying the breakdown of allelic exclusion.
Intracytoplasmic mu-positive pre-B cells (mu+VH315- cells) transformed with Abelson virus continuously produced cells (mu+VH315+ cells) that were double-stained by anti-mu and anti-VH315 antibodies which specifically recognized the immunoglobulin heavy chain variable region identical or closely related to that of MOPC315 myeloma protein. Southern blot analysis indicated that the mu+VH315+ cells were generated from the mu+VH315- cells by the VH315.D.JH2 to a germline JH3 joining on the non-productive VH315.D.JH2 allele, which resulted in the production of mu-chains with variable region detectable by anti-VH315 antibodies. Therefore, it is strongly indicated that VHDJH to JH joining is not blocked in mu-chain-producing pre-B cells, and thus is free from allelic exclusion machinery.
Sugiyama
H
H
Third Department of Internal Medicine, Osaka University Medical School, Japan.
Komori
T
T
Minami
Y
Y
Sakato
N
N
D000011:Abelson murine leukemia virus; D000483:Alleles; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002461:Cell Line, Transformed; D015870:Gene Expression; D005803:Genes, Immunoglobulin; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D007125:Immunogenetics; D007148:Immunoglobulin mu-Chains / Q000096:biosynthesis / Q000235:genetics
D007148:Immunoglobulin mu-Chains