Changes of a high molecular mass proteinase activity during Bufo bufo development.
1. A chymotrypsin-like proteolytic activity was found both in unfertilized eggs and in embryos of Bufo bufo. 2. A dramatical change of activity can be observed in the course of embryonic development. The activity rapidly increases after fertilization up to the stage 9 followed by a fall to a level close to unfertilized eggs. 3. Gel chromatography analysis reveals, in all stages of development, the presence of a single peak of proteinase activity characterized by a very high molecular mass. 4. Proteinase activity, found change during the development of Bufo bufo, was characterized by substrate specificity, protease inhibitor and pH effect. All results obtained suggest that the chymotrypsin-like activity can be assigned to the multicatalytic proteinase.
Di Cola
D
D
Istituto di Scienze Biochimiche, Facoltá di Medicina, Universitá G. D'Annunzio, Chieti, Italy.
Miranda
M
M
Bonfigli
A
A
Zarivi
O
O
Santarone
S
S
Sacchetta
P
P
D000595:Amino Acid Sequence; D000818:Animals; D002023:Bufo bufo / Q000196:embryology*; D002850:Chromatography, Gel; D002918:Chymotrypsin / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000378:metabolism*; D010063:Ovum / Q000201:enzymology; D010455:Peptides / Q000378:metabolism; D011480:Protease Inhibitors / Q000494:pharmacology; D046988:Proteasome Endopeptidase Complex; D013379:Substrate Specificity
D009097:Multienzyme Complexes; D010455:Peptides; D011480:Protease Inhibitors; D002918:Chymotrypsin; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex