[Mechanism of treating rheumatoid arthritis with polyglycosides of Tripterygium wilfordii Hook (T II). III. Study on inhibitory effect of T II on in vitro Ig secreted by peripheral blood mononuclear cells from normal controls and RA patients].
The in vitro effects of polyglycosides of Tripterygium wilfordii Hook (T II) on immunoglobulin (Ig) production by peripheral blood mononuclear cells (PB-MC) from seventeen healthy persons and eight rheumatoid arthritis (RA) patients were studied. PBMCs, monocytes and lymphocytes were treated separately with different doses of T II for different periods of time. Cells in different combinations were cultured for two weeks under pokeweed mitogen (PWM) stimulation and the IgG and IgM content of culture supernatants were determined using the double antibody solid-phase immunoradiometric assay. There was no difference in the inhibitory effect of T II on in vitro IgM and IgG production by PB-MCs either between the two types of Ig or between healthy individuals and RA patients. T II acts both on monocytes and lymphocytes, but more strongly on the latter. This observation appears to differ from the reported actions of gold and penicillamine. The results obtained in this study correlate well with those of clinical trials, suggesting that the therapeutic effect of T II is associated with its immunosuppressive activity.
Ye
W H
WH
PUMC Hospital, Beijing.
D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D006027:Glycosides / Q000494:pharmacology*; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D007075:Immunoglobulin M / Q000378:metabolism; D015592:Immunoradiometric Assay / Q000379:methods; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D029757:Tripterygium
D004365:Drugs, Chinese Herbal; D006027:Glycosides; D007074:Immunoglobulin G; D007075:Immunoglobulin M