Activation of human NK cells and monocytes with cisplatin in vitro.
Human natural killer (NK) cells and monocytes treated in vitro concomitantly with cisplatin and rIFN-gamma enhanced lysis of K562 cells. Lysis was dependent upon the duration of treatment. Cisplatin and rIFN-gamma treated monocytes were equally cytotoxic to NK sensitive (K562) and NK resistant (Daudi & Raji) cell lines whereas NK cells were not rendered cytotoxic against NK resistant tumor cells. NK- and monocyte-mediated cytotoxicity against K562 cells was further enhanced when the effector cells were primed with rIFN-gamma and were subsequently treated with cisplatin.
Sodhi
A
A
Department of Zoology, Banaras Hindu University, Varanasi, India.
Pai
K
K
Singh
R K
RK
Singh
S M
SM
D002945:Cisplatin / Q000008:administration & dosage / Q000494:pharmacology*; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004357:Drug Synergism; D006801:Humans; D007155:Immunologic Factors; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000008:administration & dosage; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology; D011994:Recombinant Proteins
D007155:Immunologic Factors; D011994:Recombinant Proteins; D007371:Interferon-gamma; D002945:Cisplatin