cDNA cloning and sequencing for the import precursor of coupling factor 6 in H(+)-ATP synthase from rat liver mitochondria.
The nucleotide sequence of the import precursor of coupling factor 6 (factor 6) of rat liver H(+)-ATP synthase has been determined from a recombinant cDNA clone isolated by screening a rat liver cDNA library with a probe DNA. The sequence was composed of 458 nucleotides including a coding region for the import precursor of factor 6 and noncoding regions of both the 5'- and 3'-sides. The import precursor of factor 6 and its mature polypeptide deduced from the open reading frame consisted of 108 and 76 amino acid residues with a molecular weight of 12,494 and 8,927, respectively. The presequence of 32 amino acids could be the import signal peptide which serves to direct the protein into the mitochondrial matrix.
Higuti
T
T
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Osaka
F
F
Yoshihara
Y
Y
Tsurumi
C
C
Kawamura
Y
Y
Tani
I
I
Toda
H
H
Kakuno
T
T
Sakiyama
F
F
Tanaka
K
K
D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics*; D004792:Enzyme Precursors / Q000235:genetics*; D015723:Gene Library; D008930:Mitochondria, Liver / Q000201:enzymology*; D025261:Mitochondrial Proton-Translocating ATPases; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010086:Oxidative Phosphorylation Coupling Factors; D011487:Protein Conformation; D021382:Protein Sorting Signals / Q000235:genetics; D006180:Proton-Translocating ATPases / Q000235:genetics* / Q000302:isolation & purification; D051381:Rats; D015183:Restriction Mapping
D004792:Enzyme Precursors; D015345:Oligonucleotide Probes; D010086:Oxidative Phosphorylation Coupling Factors; D021382:Protein Sorting Signals; D004247:DNA; D000251:Adenosine Triphosphatases; C000628217:F(6) ATPase; D025261:Mitochondrial Proton-Translocating ATPases; D006180:Proton-Translocating ATPases