[Effect of endothelin on the proliferation of aortic smooth muscle cells in normotensive and spontaneously hypertensive rats].
In order to examine the effect of endothelin (ET-1) as a growth factor, vascular smooth muscle cells were isolated from aortas of spontaneously hypertensive (SHR) and control (WKY) rats. Cell proliferation was determined by measurement of labelled 3H-thymidine incorporation in quiescent cells in presence of ET-1 alone or in association with another growth factor, insulin (INS). ET-1 alone (0.1 to 100 nM) increased slightly the growth of both types of cells. This effect was enhanced in presence of INS (0.1 to 10 micrograms/ml). SHR cells were more reactive than control ones. Activation of Na+/H+ exchange which is a necessary response for mitogenesis was dose dependently observed with increasing concentrations of ET-1 (0.1 to 100 nM) further confirming that ET-1 could act as a growth factor for vascular smooth muscle cells.
Lonchampt
M O
MO
Institut Henri Beaufour, Les Ulis, France.
Goulin
J
J
Pinelis
V
V
Chabrier
P E
PE
Braquet
P
P
D000818:Animals; D004247:DNA / Q000096:biosynthesis; D016232:Endothelins / Q000502:physiology*; D004730:Endothelium, Vascular / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D006973:Hypertension / Q000503:physiopathology; D008297:Male; D024510:Muscle Development; D009131:Muscle, Smooth, Vascular / Q000254:growth & development*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012964:Sodium / Q000378:metabolism
D016232:Endothelins; D004247:DNA; D012964:Sodium
Effet de l'endothéline sur la prolifération de cellules musculaires lisses d'aorte de rats normotendus et spontanément hypertendus.