title: Class II MHC antigen-positive macrophages from human placentae suppress strong MLR and CML reactions.
abstract: Placental adherent cells (PAC), derived by mild enzymic digestion of human placentae and adherence to plastic, were tested for their ability to suppress MLR and CML reactions. Stimulation of allogeneic T cells by cord blood mononuclear cells or by a strongly stimulatory B lymphoblastoid cell line were consistently inhibited by all PAC preparations tested. PAC were fractionated by Fc rosetting and Percoll gradients to produce a number of bands. Suppression correlated with the content of macrophages in each band and was strongest in band 5 which generally contained 94-100% macrophages. The suppressive effect was not inhibited by indomethacin. The possible role of macrophages in suppressing immune reactivity in the placenta is discussed.
authors: Department of Microbiology, University of Melbourne, Parkville, Victoria, Australia.
authors: S J
authors: SJ
authors: Uren
authors: W
authors: W
authors: Boyle
mesh_terms: D002469:Cell Separation; D003602:Cytotoxicity, Immunologic; D006681:HLA-D Antigens / Q000032:analysis; D006801:Humans; D007108:Immune Tolerance; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D007959:Lymphocyte Culture Test, Mixed; D008264:Macrophages / Q000276:immunology*; D010920:Placenta / Q000276:immunology*; D011961:Receptors, Fc / Q000032:analysis
chemical_list: D006681:HLA-D Antigens; D011961:Receptors, Fc; D007213:Indomethacin