title: Tissue-specific expression of rat c-ros-1 gene and partial structural similarity of its predicted products with sev protein of Drosophila melanogaster.
abstract: The expression and predicted products of rat c-ros-1 gene, the proto-oncogene of v-ros in UR2 sarcoma virus, were characterized. The c-ros-1 gene was found to be expressed in a tissue-specific manner, and the sizes of its transcripts were heterogeneous: 8.2 kilobases (kb) long in lung and kidney tissues, 6.9 kb in heart tissue, and 2.4 kb and 1.9 kb in testis tissue. The c-ros-1 cDNAs were isolated from lung and heart tissues. The predicted product of the c-ros-1 gene in lung tissue was a receptor-type tyrosine kinase 2,317 amino acids long (including a very large extracellular domain of approximately 1,800 amino acids) which showed a partial but significant structural homology with the sev gene product of Drosophila melanogaster. An alternatively sliced lung transcript was found to encode a protein with external and transmembrane domains but not a tyrosine kinase catalytic domain. The predicted product in heart tissue was essentially identical to that in lung tissue except for a shorter amino-terminal region and a 21-amino-acid insertion in the extracellular domain. On the basis of these results, the c-ros-1 gene appears to be active in the lungs and kidneys and probably in the hearts of rats.
authors: Department of Genetics, University of Tokyo, Japan.
authors: H
authors: H
authors: Matsushime
authors: M
authors: M
authors: Shibuya
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015342:DNA Probes; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D005136:Eye Proteins / Q000235:genetics*; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015513:Oncogene Proteins / Q000235:genetics*; D011487:Protein Conformation; D011505:Protein-Tyrosine Kinases; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D011519:Proto-Oncogenes; D051381:Rats; D011916:Rats, Inbred F344; D020794:Receptor Protein-Tyrosine Kinases; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D015342:DNA Probes; D029721:Drosophila Proteins; D005136:Eye Proteins; C000715470:MAS1 protein, human; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D015513:Oncogene Proteins; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; C062333:ROS1 protein, human; D020794:Receptor Protein-Tyrosine Kinases; C483815:Ros1 protein, rat; C056737:sev protein, Drosophila