[Studies of antigens related to metabolic and growth inhibitions of Staphylococcus aureus L-form].
Metabolic and growth inhibiting activities in immunoglobulin (of anti-S. aureus L-form serum and anti-S. aureus coccal form serum) could be absorbed by cell membranes of S. aureus L-form and its coccal form, respectively. These activities could not be absorbed by cell membrane of Micrococcus luteus, Streptococcus pyogenes or Actinomyces viscosus. These findings suggested the existence of species-specific antigens of cell membrane. The membrane antigens of L-form related to the metabolic and growth inhibiting activities were stable to trypsin, heating and periodate, and were not solubilized by trypsin. A large part of the antigen in a typsin-insoluble membrane precipitate of L-form could be extracted by acetone and the subsequent use of chloroform-methanol (2: 1). A fractionation study of chloroform-methanol extract by using silicic acid calum indicated that more than two components were involved in metabolic and growth inhibiting activities.
Hirachi
Y
Y
Department of Microbiology and Oral Microbiology, Osaka University Faculty of Dentistry.
Kotani
S
S
D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D007136:Immunoglobulins / Q000276:immunology*; D007740:L Forms / Q000276:immunology; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000276:immunology* / Q000378:metabolism
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D007136:Immunoglobulins