Demonstration of actin in neurosecretory cells by treatment with nitrobenzoxadiazole-phallacidin.
Serial sections containing neurosecretory cells from chicken hypothalamus were cut after fixation in formaldehyde and embedding in paraffin. Sections were exposed to NBD-Ph (nitrobenzoxadiazole-phallacidin) and showed evidence of containing actin. By using a medium with sodium borohydride, non-specific fluorescence could be excluded.
De Castro
R R
RR
Departamento de Morfologia, Centro Biomédico, UFES, Vitória, Es, Brasil.
Musso
F
F
Novaes
J C
JC
Novaes
M A
MA
D000199:Actins / Q000378:metabolism*; D000546:Amanitins; D000818:Animals; D002645:Chickens; D005404:Fixatives; D005456:Fluorescent Dyes; D007031:Hypothalamus / Q000737:chemistry*; D009490:Neurosecretory Systems / Q000737:chemistry*
D000199:Actins; D000546:Amanitins; D005404:Fixatives; D005456:Fluorescent Dyes; C028877:7-nitrobenz-2-oxa-1,3-diazole-phallacidin