Identification of hydroxyalkenals formed from omega-3 fatty acids.
The highly toxic lipid peroxidation product, 4-hydroxynonenal, is formed from the decomposition of hydroperoxides of omega-6 fatty acids. In this study the analogous hydroxyalkenals formed from the decomposition of hydroperoxides of omega-3 fatty acids (eicosapentaenoic acid and docosahexaenoic acid) were isolated and identified using TLC densitometry, HPLC and GC/Mass Spectrometry. The major hydroxyalkenal formed from both fatty acids was a diene analog of 4-hydroxynonenal, 4-hydroxynona(2,6)dienal, while 4-hydroxyhexanal was a minor product. Measurement of specific omega-3 lipid peroxidation products may be important in studies using dietary fish oil.
Beckman
J K
JK
Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN 37232.
Howard
M J
MJ
Greene
H L
HL
D000447:Aldehydes / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D015118:Eicosapentaenoic Acid / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D015227:Lipid Peroxidation
D000447:Aldehydes; C064453:4-hydroxyhexanal; C064454:4-hydroxynona-2,6-dienal; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid