Interleukin 3 alone does not support the proliferation of bone marrow cells from A/J mice: a novel system for studying the synergistic activities of IL-3.
The number of colonies produced by bone marrow cells in response to interleukin 3 (IL-3) in soft agar cultures varies according to the strain of the donor mice. A/J, AKR, A.TH and A.TL bone marrow cells are particularly hyporesponsive, producing only occasional colonies in the presence of IL-3. Bone marrow cells from all strains of mice, including A/J, produce distinctively large colonies in response to the combination of IL-3 and macrophage colony stimulating factor (M-CSF). In cultures of A/J bone marrow cells, the synergy between IL-3 and M-CSF is further reflected in an increase in both the number and the variety of colonies produced. The increase in colony numbers may be due to the priming of a population of A/J colony-forming-cells (CFCs) by IL-3, enabling them to respond to M-CSF. In support of this notion, IL-3 enhanced the level of c-fms (M-CSF receptor) messenger RNA in cultures of A/J bone marrow cells. It is also possible that a subpopulation of CFCs requires both IL-3 and M-CSF as co-mitogens. The A/J strain provides a novel system for studying the mechanisms involved in the interaction between IL-3 and M-CSF in haemopoiesis.
Morris
C F
CF
Division of Clinical Sciences, John Curtin School of Medical Research, Australian National University, Woden Valley Hospital, Garran, A.C.T.
Salisbury
J
J
Kobayashi
M
M
Townsend
P V
PV
Hapel
A J
AJ
D000818:Animals; D001854:Bone Marrow Cells; D002455:Cell Division; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000235:genetics / Q000494:pharmacology*; D004357:Drug Synergism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000494:pharmacology; D007377:Interleukin-3 / Q000494:pharmacology*; D016173:Macrophage Colony-Stimulating Factor; D008264:Macrophages / Q000166:cytology; D051379:Mice; D008805:Mice, Inbred A; D008815:Mice, Inbred Strains; D011518:Proto-Oncogene Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D016186:Receptor, Macrophage Colony-Stimulating Factor; D013045:Species Specificity
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007377:Interleukin-3; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D016186:Receptor, Macrophage Colony-Stimulating Factor