Effects of phosphorylation, magnesium, and filament assembly on actin-activated ATPase of pig urinary bladder myosin.
The relationship between the light-chain phosphorylation and the actin-activated ATPase activity of pig urinary bladder myosin was either linear or nonlinear depending on the free Mg2+ concentration. Varying the free [Mg2+] in the presence of 50 mM ionic strength (I) had a biphasic effect on the actin-activated ATPase. In 100 mM I, the activity increased on raising the free [Mg2+]. The activity of the phosphorylated myosin was 3-23-fold higher than that of the unphosphorylated myosin at all concentrations of free Mg2+, pH, and temperature used in this study. The increase in the turbidity and sedimentability of both phosphorylated and unphosphorylated myosins on raising the free [Mg2+] was associated with a rise in the actin-activated ATPase activity. However, myosin light-chain phosphorylation still had a remarkable effect on the actin activation. The myosin polymers formed under these conditions were sedimented by centrifugation. Experiments performed with myosin polymers formed in mixtures of unphosphorylated and phosphorylated myosins showed that the presence of phosphorylated myosin in these mixtures had a slight effect on the sedimentation of the unphosphorylated myosin but it had no effect on the actin-activated ATP hydrolysis. Electron microscopy showed that the unphosphorylated myosin formed unorganized aggregates while phosphorylated myosin molecules assembled into bipolar filaments with tapered ends. These data show that although the unphosphorylated and phosphorylated myosins have the same level of sedimentability and turbidity, the filament assembly present only with the phosphorylated myosin can be associated with the maximal actin activation of Mg-ATPase.
Samuel
M
M
Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104-6008.
Chowrashi
P K
PK
Pepe
F A
FA
Chacko
S
S
D000199:Actins / Q000494:pharmacology; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology; D008854:Microscopy, Electron; D009218:Myosins / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D010766:Phosphorylation; D011487:Protein Conformation; D013552:Swine; D001743:Urinary Bladder / Q000378:metabolism*
D000199:Actins; D017301:Ca(2+) Mg(2+)-ATPase; D009218:Myosins; D008274:Magnesium