Effect of growth hormone and serum on the expression of the proto-oncogenes c-jun and c-fos in insulin producing cells.
Expression of the proto-oncogenes c-fos and c-jun was analysed in the insulin producing rat tumor cell line, RIN 5AH. Addition of fetal calf serum (FCS) to serum-starved cells in the presence of cycloheximid induced a modest increase in c-fos and c-jun mRNA levels, whereas growth hormone (GH) in the presence of cycloheximid had little or no effect, when added to RIN 5AH cells maintained in 0.5% FCS for 2 days prior to stimulation. Activation of protein kinase C by phorbol ester lead to increased c-jun and c-fos mRNA levels, whereas activation of adenylate cyclase by forskolin increased c-fos mRNA levels. These results suggest that the effects of GH on insulin producing cells are not mediated by activation of c-fos and c-jun transcription.
Petersen
E D
ED
Hagedorn Research Laboratory, Gentofte.
Billestrup
N
N
Nielsen
J H
JH
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D001769:Blood; D005576:Colforsin / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D015342:DNA Probes; D004268:DNA-Binding Proteins / Q000235:genetics*; D004789:Enzyme Activation; D005786:Gene Expression Regulation; D007328:Insulin / Q000096:biosynthesis*; D007340:Insulinoma / Q000378:metabolism*; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D013002:Somatomedins / Q000494:pharmacology*; D014157:Transcription Factors / Q000235:genetics*; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D004268:DNA-Binding Proteins; D007328:Insulin; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D013002:Somatomedins; D014157:Transcription Factors; D005576:Colforsin; D015240:Phorbol 12,13-Dibutyrate; D003513:Cycloheximide; D011505:Protein-Tyrosine Kinases; D011493:Protein Kinase C; D000262:Adenylyl Cyclases