Changes associated with metastasis in B16-F1 melanoma cells surviving heat.
Metastasis to distant sites is mediated by various receptors on the surface of tumor cells. B16-F1 melanomas surviving 43.5 degrees C heat in vitro for 15 minutes and cultured for 10 days bind significantly increased amounts of the basement membrane protein laminin. Motility of heat-resistant B16-F1 cells in vitro toward the chemoattractant laminin is significantly increased. The increased expression of putative laminin receptors may be associated with increased metastasis of melanomas after subcurative hyperthermia.
Nathanson
S D
SD
Department of Surgery, Henry Ford Hospital, Detroit, Mich. 48202.
Cerra
R F
RF
Hetzel
F W
FW
Zarbo
R J
RJ
Crissman
J D
JD
Page
R
R
Anaya
P
P
Westrick
P
P
D002470:Cell Survival; D002633:Chemotaxis; D005455:Fluorescent Antibody Technique; D006358:Hot Temperature; D006801:Humans; D007797:Laminin / Q000032:analysis*; D008545:Melanoma / Q000473:pathology* / Q000556:secondary; D011485:Protein Binding; D011946:Receptors, Antigen / Q000032:analysis*; D011971:Receptors, Immunologic / Q000032:analysis*; D017474:Receptors, Laminin; D014407:Tumor Cells, Cultured
D007797:Laminin; D011946:Receptors, Antigen; D011971:Receptors, Immunologic; D017474:Receptors, Laminin