[The effect of heat shock on mRNA expression in human T lymphocytes].
Total mRNA was extracted from activated T lymphocytes and Jurkat cells with and without heat shock, and then used for alpha-32P-labeled 1st strand cDNA synthesis with reverse transcriptase. DNA restriction fragments or cloned vectors of five oncogenes (abl, myc, myb, fos, Ki-ras) and of IL-2, IL-2 receptor, T-cell receptor beta-chain and transferrin receptor were dotted onto nitrocellulose filters. Hybridization results showed that the expression of c-myc and TfR mRNA was much lower in heat-shocked cells than in their normal counterparts. However IL-2 and Ki-ras mRNA increased after heat shock. Possible explanations for the results are discussed.
Liu
Z
Z
Institute of Basic Medical Sciences, Beijing.
D015973:Gene Expression Regulation, Leukemic; D016259:Genes, myc; D006358:Hot Temperature; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics; D015458:Leukemia, T-Cell / Q000235:genetics*; D008213:Lymphocyte Activation; D011519:Proto-Oncogenes; D012333:RNA, Messenger; D011990:Receptors, Transferrin / Q000235:genetics; D013601:T-Lymphocytes / Q000737:chemistry*
D007376:Interleukin-2; D012333:RNA, Messenger; D011990:Receptors, Transferrin