Comparative antiproliferative and receptor binding activities of interferons alpha and beta on lymphoblastoid and melanoma cells.
The relative antiproliferative and receptor binding characteristics of the hitherto little-characterized interferon alpha 4a on cells of lymphoid and epithelial origin are compared with two other type I interferons, alpha 2 a and beta. Using the lymphoblastoid cell line, Daudi, interferons alpha 4 a and alpha 2 b had similar antiproliferative activity, and were about 10-fold more active than IFN beta. By contrast, using the melanoma cell line Sk-Mel-28, IFN beta was the most active, whereas IFN alpha 2b and IFN alpha 4a were respectively 60-fold and greater than 1000-fold less active than on Daudi cells. Receptor binding did not correlate with antiproliferative sensitivities, but confirmed a shared receptor component for these three interferons. These results indicate that the antiproliferative activities of three type I IFNs differs markedly on different cell types and that this is unlikely to be due to receptor binding, but more likely a post receptor binding event.
Hertzog
P J
PJ
Centre for Molecular Biology & Medicine Monash University, Clayton, Victoria, Australia.
Johns
T G
TG
Callister
K A
KA
Dinatale
A
A
Linnane
A W
AW
D001667:Binding, Competitive; D002051:Burkitt Lymphoma / Q000378:metabolism / Q000473:pathology*; D002455:Cell Division; D006801:Humans; D007370:Interferon Type I / Q000378:metabolism / Q000494:pharmacology*; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000378:metabolism / Q000494:pharmacology; D008545:Melanoma / Q000378:metabolism / Q000473:pathology*; D011971:Receptors, Immunologic / Q000378:metabolism*; D017471:Receptors, Interferon; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured
D007370:Interferon Type I; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D011994:Recombinant Proteins; C068567:interferon alpha 4A