[Curing effects of chlorination, ozone and UV treatments on plasmid DNAs].
Curing effects of chlorine, ozone and ultraviolet (UV) on plasmid DNAs were investigated as one of the measures of bio-hazards due to recombinant plasmids. For donor strains of plasmids, Pseudomonas aeruginosa RM 2046 harboring the self-transmissible plasmid R68 and RM 2021 harboring the cloning vector R1162, Escherichia coli C 600 ML 4903 and ML 1410 harboring the self-transmissible plasmid Rts-1 and RP4, and E. coli JM 109 harboring the cloning vector pBR 322 were used. The curing rate showed a tendency to increase with treatment time in a L-shaped curve for each plasmid DNA. The rates reached steady state after more than 5-10 minutes of the chlorination and ozone treatment, or 5-10 seconds of the UV treatment, independent of the treatment intensity. It became clear by use of replica technique that effective curing took place by chlorination for Rts-1, R1162 and pBR322, by ozone treatment for R68, and by UV treatment for PR4, respectively. Of the three disinfection treatments excellent curing effect occurred with chlorination, as more than 90% of the curing rate was obtained by that treatment for all donor strains.
Nakamura
S
S
Department of Food and Nutrition, Ube College.
D002713:Chlorine; D003264:Containment of Biohazards / Q000379:methods*; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000235:genetics; D013464:Sulfuric Acids; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D013464:Sulfuric Acids; D002713:Chlorine; C048813:potassium peroxymonosulfuric acid