[Purification and various properties of Vibrio cholerae NON-01 cytolysin].
A new method for isolation and purification of Vibrio cholerae non-01 cytolysin has been elaborated. It includes the steps of concentration by ammonium sulphate, ion exchange chromatography on DE-52-cellulose, gel filtration via ultragel AsA-44 and chromatography on Mono Q. Cytolysin is shown to be a 60 kD and pI 6.2 protein. It is not inactivated by thiol-restoring agents and induces the production of precipitating antibodies. In contrast to choleragen the protein cytolytically affects erythrocytes and cells of the line L-929. It also possesses the lethal and oedematous effect and demonstrates the enteropathogenic effect on nursing rabbits.
Tsittser
A O
AO
Ezepchuk
Iu V
IuV
Nakisbekov
N O
NO
Li
A V
AV
Semiotrochev
V L
VL
Kiselev
Iu L
IuL
D000818:Animals; D002852:Chromatography, Ion Exchange; D003603:Cytotoxins / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D007928:Lethal Dose 50; D051379:Mice; D011817:Rabbits; D014734:Vibrio cholerae / Q000378:metabolism*
D003603:Cytotoxins
Ochistka i nekotorye svoĭstva tsitolizina Vibrio cholerae NON-01.