[Cloning of alpha-hemolysin gene from Staphylococcus aureus strain 015].
The genomic library of Staphylococcus aureus O15 has been constructed on the EMBL-3 vector. The synthetic oligonucleotide probes to N- and C-end regions of alpha-hemolysin permitted identification of the recombinant bacteriophage clone RS-1 containing a gene for this protein. The restriction map of the cloned fragment has been constructed for restriction endonucleases SalGI, EcoRV, PstI, PvuII. Expression of the alpha-hemolysin gene in phagolysate of the recombinant clone RS-1 (1000 units per ml) has been demonstrated.
Meshveliani
D K
DK
Adamiia
R Sh
RSh
Vashakidze
R P
RP
Mamulashvili
N A
NA
Kvachadze
L I
LI
Ogarkova
O A
OA
D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes; D005798:Genes, Bacterial; D005822:Genetic Vectors; D006460:Hemolysin Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D013204:Staphylococcus Phages / Q000235:genetics; D013211:Staphylococcus aureus / Q000235:genetics*
D015342:DNA Probes; D006460:Hemolysin Proteins
Klonirovanie gena alpha-gemolizina shtamma Staphylococcus aureus 015.