title: Expression of a Mr 32,000 laminin-binding protein messenger RNA in human colon carcinoma correlates with disease progression.
abstract: Cell surface receptors for laminin may play an important role in tumor migration and metastasis. To evaluate laminin receptor/laminin-binding protein expression in human colon carcinoma, surgical specimens of primary colon cancers and liver metastases were examined by blot hybridization of total RNA with a complementary DNA clone which encodes a Mr 32,000 human laminin-binding protein. The mRNA level of the laminin-binding protein was higher in primary colon carcinoma than in adjacent normal colonic epithelium in 20 of 21 cases. In all 6 cases of colon cancer liver metastases, the laminin-binding protein mRNA level was more than 3-fold greater in tumor than in adjacent normal liver tissue. The tumor/normal ratio of this laminin-binding protein mRNA expression in primary colon cancer has significant correlation with Dukes' classification (P less than 0.001). Our results suggest that mRNA expression of the laminin-binding protein may be a marker of human colorectal cancer progression and biological aggressiveness.
authors: Department of Surgery, New England Deaconess Hospital, Boston, Massachusetts 02215.
authors: K
authors: K
authors: Mafune
authors: T S
authors: TS
authors: Ravikumar
authors: J M
authors: JM
authors: Wong
authors: H
authors: H
authors: Yow
authors: L B
authors: LB
authors: Chen
authors: G D
authors: GD
authors: Steele
mesh_terms: D000230:Adenocarcinoma / Q000032:analysis* / Q000473:pathology; D000368:Aged; D000369:Aged, 80 and over; D003110:Colonic Neoplasms / Q000032:analysis* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D012333:RNA, Messenger / Q000032:analysis*; D012334:RNA, Neoplasm / Q000032:analysis*; D011971:Receptors, Immunologic / Q000235:genetics*; D017474:Receptors, Laminin
chemical_list: D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011971:Receptors, Immunologic; D017474:Receptors, Laminin