Identification of an interleukin-1 beta binding protein in human plasma.
A covalent cross-linking technique was used to bind iodinated interleukin-1 (IL1) alpha and beta to plasma proteins. One specific IL1 beta binding protein was observed, that when cross-linked to 125I-IL1 beta migrated to approximately 60 kDa on SDS-PAGE. The protein did not bind IL1 alpha. The 43 -kDa protein was partially purified using a wheat germ agglutinin affinity column. The isolated factor again specifically bound IL1 beta, and appeared to consist of single chain glycoprotein. The protein was heat stable and had a rapid association time with IL1 beta. This protein may be an important carrier molecule for IL1 beta in vivo.
Eastgate
J A
JA
University of Edinburgh Department of Medicine, RDU, Northern General Hospital, Scotland.
Symons
J A
JA
Duff
G W
GW
D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D002352:Carrier Proteins / Q000032:analysis* / Q000502:physiology; D002460:Cell Line; D002846:Chromatography, Affinity; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification / Q000502:physiology; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis* / Q000502:physiology; D011971:Receptors, Immunologic / Q000032:analysis*; D017472:Receptors, Interleukin-1; D012995:Solubility
D001798:Blood Proteins; D002352:Carrier Proteins; D003432:Cross-Linking Reagents; D006023:Glycoproteins; D007375:Interleukin-1; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1