Analysis of human major histocompatibility complex antigens by electrophoretic methods with subsequent immunoblotting.
A fast, simple, and sensitive technique is described for the biochemical analysis of human MHC class I antigens. It is based on 2-D electrophoresis or 1-D isoelectric focusing, followed by immunoblotting. The need for radiolabelling of the cells is eliminated, and because whole cell lysates can be used the number of cells needed for analysis is reduced tenfold over conventional immunoprecipitation techniques. The method can also be used to analyze certain MHC class II antigens.
Serdyuk
O A
OA
All-Union Research Institute of Biotechnology, Ministry of Medical Industry of the USSR, Moscow.
Vasilov
R G
RG
D015153:Blotting, Western / Q000379:methods*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006680:HLA Antigens / Q000032:analysis*; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D008214:Lymphocytes / Q000276:immunology
D006680:HLA Antigens; D006684:HLA-DR Antigens