A simple method for coating of liposomes with proteins.
A rapid and simple method for the coating of liposomes with proteins is described. Many proteins (e.g. bovine serum albumin, pig gammaglobulins and tetanus toxoid) could be made to adhere rapidly and efficiently to both multilamellar and small unilamellar vesicles after treatment with equimolar NaNO2 and HCl/NaCl. The reaction is complete within four hours and almost all of the protein is retained on the liposomes after storage for 2 weeks at 4 degrees C.
Tan
L S
LS
Department of Pharmacology, Faculty of Medicine, National University of Singapore.
Gregoriadis
G
G
D006851:Hydrochloric Acid; D007136:Immunoglobulins; D008081:Liposomes; D011506:Proteins / Q000737:chemistry*; D012710:Serum Albumin, Bovine; D012965:Sodium Chloride; D012977:Sodium Nitrite
D007136:Immunoglobulins; D008081:Liposomes; D011506:Proteins; D012710:Serum Albumin, Bovine; D012965:Sodium Chloride; D012977:Sodium Nitrite; D006851:Hydrochloric Acid