Changes in glycosaminoglycan synthesis and in heparan sulfate deposition in human colorectal adenocarcinomas.
Biosynthesis of glycosaminoglycans (GAGs) was studied in morphologically normal colonic mucosa, in peritumoral and tumoral areas, and in colorectal polyps of tumor-bearing patients. After GAG purification, overall biosynthesis was determined: the general trend was a decrease in GAG production in neoplastic colon, lowest GAG synthesis being observed in Dukes' stage C tumors. Separation by ion-exchange chromatography of various GAG species and further characterization revealed the presence of hyaluronic acid (HA) and heparan sulfate (HS) molecules in all specimens studied. Chondroitin-4 sulfate (CS4) was occasionally found in tumor samples. The relative proportion of HA and HS was modified in tumor tissue: i.e. increased HA and decreased HS were observed. Differences in DEAE-chromatographic behavior were obvious in pathological samples as compared to controls, the hydrodynamic form of HA and the charge density of HS being decreased. The latter could be attributed to undersulfatation of HS molecules. Immunocytochemical detection of HS proteoglycan molecules revealed regular and bright labelling at epithelial-stromal interface in control samples. In pathological samples, staining was patchy and discontinuous, showing large areas of basement membrane interruption.
Bouziges
F
F
Unité 61 INSERM, Strasbourg, France.
Simon-Assmann
P
P
Leberquier
C
C
Marescaux
J
J
Bellocq
J P
JP
Haffen
K
K
Kedinger
M
M
D000230:Adenocarcinoma / Q000032:analysis / Q000378:metabolism*; D011508:Chondroitin Sulfate Proteoglycans / Q000032:analysis / Q000378:metabolism; D003106:Colon / Q000032:analysis / Q000378:metabolism; D003111:Colonic Polyps / Q000032:analysis / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000032:analysis / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism*; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000032:analysis / Q000378:metabolism; D012007:Rectum / Q000032:analysis / Q000378:metabolism
D011508:Chondroitin Sulfate Proteoglycans; D006025:Glycosaminoglycans; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate