Analysis of T cell receptor V beta gene usage in primary mixed lymphocyte reactions: evidence for directive usage by different antigen-presenting cells and Mls-like determinants on T cell blasts.
The usage of four different T cell receptor (TcR) V beta gene families within normal, non-primed T cell populations in response to various types of antigen-presenting cells (APC) in primary mixed lymphocyte reaction has been studied. We demonstrate that distinct patterns of V beta gene usage are obtained within a given T cell population in response to different types of APC with the same allo-H-2. When responder T cells are stimulated with one type of allogeneic APC, from various H-2-disparate mice, the same V beta gene preference is observed. Furthermore, when H-2- and Mls-mismatched APC gene used as stimulators, the Mls-associated V beta 6 and V beta 8.1 gene families are highly elevated in response to both B and T cell blasts from certain Mls-positive strains. The results demonstrate that different types of allogeneic APC have the capacity to generate biases in TcR V beta gene usage and imply that functional Mls-like determinants are presented by T cell blasts. The findings are discussed with respect to TcR-major histocompatibility complex interactions in allostimulation.
Larsson-Sciard
E L
EL
Unité de Biologie Moléculaire du Gène, U.277 INSERM, UAC 115 CNRS, Institut Pasteur, Paris, France.
Spetz-Hagberg
A L
AL
Casrouge
A
A
Kourilsky
P
P
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000954:Antigens, Surface / Q000502:physiology*; D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D005838:Genotype; D015395:Histocompatibility Antigens Class I / Q000502:physiology; D066298:In Vitro Techniques; D007959:Lymphocyte Culture Test, Mixed; D008285:Major Histocompatibility Complex / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D016747:Minor Lymphocyte Stimulatory Antigens; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000502:physiology*; D016693:Receptors, Antigen, T-Cell, alpha-beta; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*
D000954:Antigens, Surface; D015395:Histocompatibility Antigens Class I; D016747:Minor Lymphocyte Stimulatory Antigens; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta