An antibody-tumor model for the targeting of CA125-producing gynecologic malignancies.
By immunizing a mouse with HOUA-1 cells established from an endometrial cancer patient, two murine monoclonal antibodies designated 196-14 and 196-28 were generated, which were reactive with ovarian cancer-associated antigen CA125, originally defined by OC125 antibody. Antigenic determinants of these antibodies, although overlapping each other, were different from that of OC125 and the combined use of 125I-labeled 196-14 and OC125-coated beads markedly increased the sensitivity of measuring CA125 antigen. Both radioiodinated and 111In-labeled 196-14 localized well in CA125-producing human ovarian cancer tissues OVA-5 xenografted in nude mice. The biodistribution of radioiodinated 196-14 was quite different from that of 111In-labeled 196-14. Radioiodine was cleared faster from the OVA-5 tumor, making a clear contrast to the prolonged retention of 111In the tumor. Initial tumor uptake of radioiodinated 196-14 was the same as that of 111In-labeled 196-14 but decreased thereafter, due to the dehalogenation of radioiodinated antibody in the tumor. This antibody-tumor model seems to be suitable for examining the usefulness of monoclonal antibody-conjugates in the diagnosis and therapy of CA125-producing endometrial or ovarian cancers.
Saga
T
T
Department of Nuclear Medicine, Kyoto University School of Medicine.
Ishiwata
I
I
Endo
K
K
Sakahara
H
H
Koizumi
M
M
Watanabe
Y
Y
Nakai
T
T
Hosono
M
M
Ishikawa
H
H
Sawada
M
M
D000230:Adenocarcinoma / Q000276:immunology; D000911:Antibodies, Monoclonal; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000096:biosynthesis*; D001667:Binding, Competitive; D003536:Cystadenocarcinoma / Q000276:immunology; D005260:Female; D005833:Genital Neoplasms, Female / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D015592:Immunoradiometric Assay; D007668:Kidney / Q000276:immunology; D008099:Liver / Q000276:immunology; D008168:Lung / Q000276:immunology; D008875:Middle Aged; D009132:Muscles / Q000276:immunology
D000911:Antibodies, Monoclonal; D015295:Antigens, Tumor-Associated, Carbohydrate