title: Flow cytometric competitive binding assay for determination of actinomycin-D concentrations.
abstract: A single step, separation free competitive binding reaction between the fluorescent antibiotic mithramycin and actinomycin-D for common binding sites on DNA coated 10 microns diameter microspheres is described. The fluorescence of the microspheres is measured with a flowcytometer. In the presence of a constant amount of mithramycin, the microsphere fluorescence is inversely proportional to actinomycin-D concentration.
authors: Cell Biology Group, Los Alamos National Laboratory, New Mexico 87545.
authors: G C
authors: GC
authors: Saunders
authors: J C
authors: JC
authors: Martin
authors: J H
authors: JH
authors: Jett
authors: A
authors: A
authors: Perkins
mesh_terms: D001667:Binding, Competitive; D003609:Dactinomycin / Q000378:metabolism*; D005434:Flow Cytometry; D008863:Microspheres; D008926:Plicamycin / Q000378:metabolism
chemical_list: D003609:Dactinomycin; D008926:Plicamycin