title: Phosphofructokinase from baker's yeast: studies on the initial kinetics and the fast reacting thiol groups of a proteolytically modified active enzyme form.
abstract: The initial kinetics as well as the reactivity of the fast reacting thiol groups of a tetrameric form of phosphofructokinase from baker's yeast (called 12 S-enzyme), obtained by limited proteolysis in the presence of ATP were studied by the stopped-flow technique. Before attaining the steady state, the reaction shows a lag phase in the product formation, the duration of which decreases with increasing enzyme concentration. The lag phase disappears after preincubation of the enzyme with either fructose 6-phosphate, fructose 1,6-bisphosphate or fructose 2,6-bisphosphate. The occurrence of an initial transient phase suggests that the enzyme converts from a state of low activity into a highly active one after starting the reaction. The modified enzyme was found to contain two fast reacting cysteinyl residues with respect to their reactivity towards 5,5'-dithiobis(2-nitrobenzoic acid). Fructose 6-phosphate, fructose 1,6-bisphosphate and fructose 2,6-bisphosphate, respectively, decrease the reactivity of this class of thiol groups but not the total number of titrable cysteins. This result supports the hypothesis of a conformational change as a consequence of the effector binding.
authors: Institut für Biochemie, Bereich Medizin, Karl-Marx-Universität Leipzig, DDR.
authors: J
authors: J
authors: Bär
authors: G
authors: G
authors: Hübner
authors: W
authors: W
authors: Schellenberger
authors: G
authors: G
authors: Kopperschläger
mesh_terms: D007700:Kinetics; D010447:Peptide Hydrolases; D010732:Phosphofructokinase-1 / Q000737:chemistry / Q000378:metabolism*; D011487:Protein Conformation; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013438:Sulfhydryl Compounds
chemical_list: D013438:Sulfhydryl Compounds; D010732:Phosphofructokinase-1; D010447:Peptide Hydrolases