A convenient assay for detection of soluble interferon receptors.
The most common problem that must be overcome in purification of plasma membrane receptors from a cell line or a tissue is the loss of activity after solubilization with a detergent. This paper describes a simple dot blot assay with 32P-labeled human interferon gamma (Hu-IFN-gamma) for the detection of a functionally active Hu-IFN-gamma receptor from soluble plasma membranes prepared from human placenta.
Stefanos
S
S
Department of Molecular Genetics and Microbiology, UMDNJ-Robert Wood Johnson Medical School, Piscataway.
Pestka
S
S
D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D008722:Methods; D010920:Placenta / Q000737:chemistry; D011247:Pregnancy; D011971:Receptors, Immunologic / Q000032:analysis*; D017471:Receptors, Interferon; D012995:Solubility
D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D007371:Interferon-gamma