title: T lymphocytes and eosinophils in allergen-induced late-phase asthmatic reactions in the guinea pig.
abstract: The kinetics and phenotype of T lymphocytes infiltrating the airways of guinea pigs undergoing late-phase asthmatic reactions (LAR) were studied with monoclonal antibodies, cytofluorimetry, and immunocytochemistry. Challenge of sensitized animals with aerosolized ovalbumin was followed by early (2 h) and late-phase (17 h) bronchoconstriction. The induction of hypersensitivity, by aerosolized antigen, was associated with an increase in mucosal T cell numbers, which consisted almost entirely of CD8+ T cells. Following allergen challenge of fully sensitized animals, a biphasic rise in total T cell (CD3+) numbers was observed in the bronchial mucosa, peaking at 17 and 48 h. A similar pattern of T cell accumulation was observed in the bronchial adventitia but with an extra early peak at 2 h. In contrast to the T cell influx of the sensitization phase, the postchallenge infiltrate consisted largely of CD3+, CD8- cells. Eosinophil numbers were elevated in both submucosa and adventitia, with a single broad peak between 17 and 48 h. T cell infiltration was compared with eosinophil accumulation; while correlations between T cell and eosinophil numbers varied over the 96 h of the experiment, strong associations were observed between CD8+ numbers and eosinophils in the adventitia at 6 h (r = 0.733, p less than 0.01) and between CD3+ numbers and eosinophils in the submucosa at 72 h (r = 0.88, p less than 0.001). No significant changes were detected in T cell or eosinophil numbers in the lung parenchyma. There was a postchallenge increase in eosinophils (but not T cells) in bronchoalveolar lavage (BAL).(ABSTRACT TRUNCATED AT 250 WORDS)
authors: Department of Allergy and Clinical Immunology, National Heart and Lung Institute, Brompton Hospital, London, U.K.
authors: A J
authors: AJ
authors: Frew
authors: R
authors: R
authors: Moqbel
authors: M
authors: M
authors: Azzawi
authors: A
authors: A
authors: Hartnell
authors: J
authors: J
authors: Barkans
authors: P K
authors: PK
authors: Jeffery
authors: A B
authors: AB
authors: Kay
authors: R J
authors: RJ
authors: Scheper
authors: J
authors: J
authors: Varley
authors: M K
authors: MK
authors: Church
mesh_terms: D000403:Airway Resistance / Q000502:physiology; D000485:Allergens / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal; D001249:Asthma / Q000209:etiology* / Q000276:immunology; D001980:Bronchi / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D002465:Cell Movement / Q000502:physiology; D004804:Eosinophils / Q000276:immunology*; D005434:Flow Cytometry; D006168:Guinea Pigs; D007114:Immunization / Q000379:methods; D008297:Male; D009092:Mucous Membrane / Q000276:immunology; D013047:Specific Pathogen-Free Organisms; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
chemical_list: D000485:Allergens; D000911:Antibodies, Monoclonal