Human p53 is phosphorylated by p60-cdc2 and cyclin B-cdc2.
The human anti-oncoprotein p53 is shown to be a substrate of cdc2. The primary site of phosphorylation is serine-315. Serine-315 is phosphorylated by both p60-cdc2 and cyclin B-cdc2 enzymes. The phosphorylation of p53 is cell cycle-dependent. The abundance of p53 also oscillates during the cell cycle. The protein is largely absent from cells that have just completed division but accumulates in cells during G1 phase. Phosphorylation by cdc2 might regulate the antiproliferative activity of p53.
Bischoff
J R
JR
Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, NY 11724.
Friedman
P N
PN
Marshak
D R
DR
Prives
C
C
Beach
D
D
D000595:Amino Acid Sequence; D000818:Animals; D049872:Bivalvia; D016203:CDC2 Protein Kinase; D002453:Cell Cycle; D002460:Cell Line; D016213:Cyclins; D006706:Homeostasis; D006801:Humans; D007447:Invertebrate Hormones / Q000378:metabolism*; D008969:Molecular Sequence Data; D015513:Oncogene Proteins / Q000302:isolation & purification / Q000378:metabolism*; D010449:Peptide Mapping; D010455:Peptides / Q000138:chemical synthesis; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D012694:Serine; D016159:Tumor Suppressor Protein p53
D016213:Cyclins; D007447:Invertebrate Hormones; D015513:Oncogene Proteins; D010455:Peptides; D010750:Phosphoproteins; D016159:Tumor Suppressor Protein p53; D012694:Serine; D011494:Protein Kinases; D016203:CDC2 Protein Kinase