Impaired aldosterone production by long-term infusion of atrial natriuretic factor.
This study assessed the effect of chronic infusions of atrial natriuretic factor (ANF) on in vivo and in vitro production of aldosterone. Vehicle (saline) or rat ANF-(99-126) was intravenously infused at 100 ng.kg-1.h-1 for 5 consecutive days into male New Zealand White rabbits. At 5 days plasma ANF was 18 +/- 4.1 pg/ml in vehicle-infused and 48.5 +/- 9.0 in ANF-infused rabbits (P less than 0.01). Plasma renin activity was significantly less in ANF-infused rabbits (2.99 +/- 0.35 vs. 0.77 +/- 0.12 ng.ml-1.h-1, P less than 0.01); however no differences were observed in the basal plasma concentrations of aldosterone, corticosterone, potassium, or hematocrit. In in vivo studies, chronically administered ANF attenuated plasma aldosterone, but not pressor, responses to acutely infused angiotensin II given at doses of 4, 16, and 64 ng.kg-1.min-1 for 20 min each. In in vitro experiments, collagenase-dispersed adrenal capsular cells from ANF-infused rabbits exhibited significantly reduced maximal responses to adrenocorticotropic hormone, angiotensin II, and potassium. These results suggest that chronic small increases in circulating ANF can blunt selectively adrenocortical responses to aldosterone secretagogues without affecting pressor responses to angiotensin II.
Nagano
M
M
Department of Heart and Hypertension, Cleveland Clinic Foundation, Ohio 44195.
Bravo
E L
EL
D000313:Adrenal Medulla / Q000187:drug effects / Q000378:metabolism; D000450:Aldosterone / Q000096:biosynthesis / Q000097:blood*; D000804:Angiotensin II / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000008:administration & dosage / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects; D003366:Cosyntropin / Q000494:pharmacology; D004232:Diuretics / Q000494:pharmacology*; D004334:Drug Administration Schedule; D007262:Infusions, Intravenous; D007700:Kinetics; D008297:Male; D000451:Mineralocorticoid Receptor Antagonists; D010446:Peptide Fragments / Q000008:administration & dosage / Q000494:pharmacology*; D011817:Rabbits; D012016:Reference Values
D004232:Diuretics; D000451:Mineralocorticoid Receptor Antagonists; D010446:Peptide Fragments; D000804:Angiotensin II; D003366:Cosyntropin; D000450:Aldosterone; D009320:Atrial Natriuretic Factor