Possibility to improve preservation of whole blood by leukocyte-depletion before storage.
In order to evaluate the influence of leukocytes in whole blood on the standard blood bank conditions for storage, we compared several parameters of red cells, leukocytes and platelets in leukocyte-rich and leukocyte-poor whole blood over a storage period of 5 weeks. The leukocyte-depleted units were prepared by filtration with a leukocyte trapping filter (Sepacell R500A). Our data indicate improved storage conditions, made evident by significantly lower cell damage: smaller increases of elastase, beta-thromboglobulin and lactate dehydrogenase in leukocyte-depleted blood. This improvement was achieved in a simple two-bag system using only filtration, without addition of rejuvenating solutions. In addition, this procedure allows greater flexibility in the use of filtrated blood for transfusions.
Riedner
C
C
Department of Medicine III, University of Munich, FRG.
Heim
M U
MU
Mempel
W
W
Wilmanns
W
W
D000255:Adenosine Triphosphate / Q000097:blood; D001793:Blood Preservation / Q000379:methods*; D002469:Cell Separation; D015926:Complement C3a / Q000378:metabolism; D004163:Diphosphoglyceric Acids / Q000097:blood; D004906:Erythrocyte Count; D005344:Fibrinopeptide A / Q000378:metabolism; D005374:Filtration; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007958:Leukocyte Count; D007962:Leukocytes; D010196:Pancreatic Elastase / Q000097:blood; D010976:Platelet Count; D001620:beta-Thromboglobulin / Q000378:metabolism
D004163:Diphosphoglyceric Acids; D001620:beta-Thromboglobulin; D005344:Fibrinopeptide A; D015926:Complement C3a; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase; D010196:Pancreatic Elastase