Effect of endogenous and exogenous polyamines on organic cation transport in rabbit renal plasma membrane vesicles.
The effect of several polyamines on the transport of organic cations [N1-[3H]methylnicotinamide (NMN), [3H]NMN and 3H-tetraethylammonium, ([3H]TEA)] in rabbit renal brush border (BBMV) and basolateral membrane vesicles (BLMV) was studied using a rapid filtration assay. Under pH-driven conditions in BBMV, [3H]NMN transport was cis inhibited approximately 30% by the naturally occurring polyamines (cadaverine, putrescine, spermine and spermidine) and nearly 90% by methylglyoxal bis(guanylhydrazone) (MGBG), a synthetic polyamine, mepiperphenidol and cimetidine, classical organic cation transport inhibitors. In the absence of a pH gradient, the capability of these agents to block [3H]NMN transport was diminished. The capability of these compounds to translocate the membrane was assessed by examining the phenomenon of counterflow. TEA, mepiperphenidol and MGBG produced trans stimulation of TEA uptake, whereas putrescine did not. Kinetic studies revealed that both putrescine and MGBG affected the Km value for TEA transport while having a minimal effect on the Vmax value. These results are most consistent with competitive inhibition. The specificity of polyamine inactivation of organic cation transport was assessed by examining what effect they had on the transport of another brush border transporter, D-glucose. These agents did not inhibit D-glucose transport. In addition, the effect of polyamines on the transport of organic cations in BLMV was studied. Only MGBG, cimetidine and mepiperphenidol inhibited organic cation transport. The results indicate that endogenous polyamines cis inhibited the organic cation transporter in BBMV competitively, whereas the exogenous polyamine MGBG cis inhibited competitively and produced trans stimulation. In contrast in BLMV, only MGBG was an effective inhibitor.
Sokol
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Department of Medicine, Indiana University School of Medicine, Indianapolis.
Gates
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D000818:Animals; D015317:Biogenic Polyamines / Q000494:pharmacology* / Q000502:physiology; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism*; D008297:Male; D008935:Mitoguazone / Q000494:pharmacology*; D009536:Niacinamide / Q000031:analogs & derivatives / Q000493:pharmacokinetics; D011817:Rabbits; D013757:Tetraethylammonium Compounds / Q000493:pharmacokinetics
D015317:Biogenic Polyamines; D013757:Tetraethylammonium Compounds; D009536:Niacinamide; D005947:Glucose; D008935:Mitoguazone; C024058:N(1)-methylnicotinamide