Effect of carbon dioxide flow-rate on the separation of triacylglycerols by capillary supercritical fluid chromatography.
Triacylglycerols (TAGs) were separated using capillary supercritical fluid chromatography at various carbon dioxide densities and flow-rates at 150 degrees C. The flow-rate was controlled with integral restrictors made directly at the end of the column. With a 50 microns I.D., df 0.20 micron, DB-5 [(95%)dimethyl(5%) diphenylpolysiloxane] column, separation numbers (Tz) of up to one per one triacylglycerol carbon number difference per metre were achieved at a practical optimum carrier flow-rate at 0.3-0.4 cm/s. Height equivalent to an effective theoretical plate-average linear carrier velocity-triacylglycerol carbon number three-dimensional plots at different pressures are presented.
Kallio
H
H
Department of Chemistry and Biochemistry, University of Turku, Finland.
Laakso
P
P
D002245:Carbon Dioxide; D002845:Chromatography / Q000379:methods*; D005410:Flame Ionization / Q000295:instrumentation; D013696:Temperature; D014280:Triglycerides / Q000032:analysis*
D014280:Triglycerides; D002245:Carbon Dioxide