Preparation and use of double-labelled enterobactin.
Double-labelled 3H/14C-enterobactin was isolated from bacterial cultures, and evaluated as a potential tool for studying the mammalian metabolism of this iron chelator. Microbial yields were low, but adequate, with a final 3H/14C ratio of 2.95 to 1. Studies conducted with mice indicated that considerable metabolism and rapid elimination of an intraperitoneally injected sample had occurred in 24 hours.
Bergstrom
C P
CP
Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois, Chicago 60612.
Lu
M C
MC
Bell
C L
CL
D000818:Animals; D002250:Carbon Radioisotopes; D004758:Enterobactin / Q000302:isolation & purification* / Q000378:metabolism; D007553:Isotope Labeling; D051379:Mice; D008815:Mice, Inbred Strains; D014316:Tritium
D002250:Carbon Radioisotopes; D014316:Tritium; D004758:Enterobactin