The metabolism of erythropoietin in the normal and uraemic rabbit.
The metabolism of recombinant human erythropoietin (rHuEpo) labelled with 125I has been investigated in five normal and nine 5/6-nephrectomised rabbits. The plasma erythropoietin half-life was significantly prolonged at 5.1 +/- 1.2 h (mean +/- SD) in the 5/6-nephrectomised rabbits, compared to 3.0 +/- 0.4 h in sham-operated controls (P less than 0.001). The disappearance of 125I-labelled rHuEpo is biphasic. Examination of serum by fast protein liquid chromatography (FPLC) following administration of 125I-labelled rHuEpo by FPLC showed a single peak of radioactivity in all rabbits except two of the nephrectomised group. In serum from both of these animals a second labelled peak was found, corresponding to material of MW 200,000-250,000 D. We conclude that the 5/6-nephrectomised rabbit provides a stable model for the study of hormonal metabolism in chronic renal failure.
Brown
J H
JH
Renal Unit, Belfast City Hospital, Northern Ireland.
Lappin
T R
TR
Elder
G E
GE
Bridges
J M
JM
McGeown
M G
MG
D000818:Animals; D004195:Disease Models, Animal; D004921:Erythropoietin / Q000097:blood*; D005260:Female; D006207:Half-Life; D066298:In Vitro Techniques; D008297:Male; D009392:Nephrectomy; D011817:Rabbits; D014511:Uremia / Q000097:blood* / Q000209:etiology
D004921:Erythropoietin