Cloning of the pyr4 gene encoding orotidine-5'-phosphate decarboxylase in Cephalosporium acremonium.
We have cloned the Cephalosporium acremonium pyr4 gene by cross-hybridization with the equivalent gene from Neurospora crassa, the closest relative from which this gene is available. The C. acremonium pyr4 gene complements an E. coli pyrF mutant lacking orotidine-5'-phosphate decarboxylase (OMPdecase), and most probably does not contain introns. Maxicell analysis in E. coli shows that it encodes a 46 kDa polypeptide. The C. acremonium OMPdecase contains a highly conserved pentadecapeptide characteristic for this category of enzyme. Extensive sequence comparison suggests an important role of this region in enzymatic activity.
Vian
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Departamento de Investigación, Antibióticos S. A., Madrid, Spain.
Peñalva
M A
MA
D000164:Acremonium / Q000201:enzymology / Q000235:genetics*; D000595:Amino Acid Sequence; D005075:Biological Evolution; D002262:Carboxy-Lyases / Q000235:genetics*; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000235:genetics; D009693:Nucleic Acid Hybridization; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D002262:Carboxy-Lyases; D009964:Orotidine-5'-Phosphate Decarboxylase