Transcription and translation of the chloroplast atpB-gene and assembly of ATP synthase subunit beta.
In vitro transcription and subsequent translation of the cloned Chlamydomonas chloroplast atpB gene was used to study assembly of ATP synthase. Translation in the presence of thylakoids resulted in association of the beta subunit with the membrane. The in vitro synthesized polypeptide bound to the membrane copurified with CF1 on sucrose gradients. This provides more evidence for the self-assembly of CF1.
Leu
S
S
Department of Biology, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Weinberg
D
D
Michaels
A
A
D002696:Chlamydomonas; D002736:Chloroplasts / Q000201:enzymology / Q000502:physiology* / Q000648:ultrastructure; D003001:Cloning, Molecular; D017343:Genes, Plant; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism; D046911:Macromolecular Substances; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D006180:Proton-Translocating ATPases / Q000235:genetics*; D014158:Transcription, Genetic
D046911:Macromolecular Substances; D006180:Proton-Translocating ATPases