[Adrenergic stimulation and renal synthesis of prostaglandins in genetically hypertensive rats of the Lyons strain].
An increased urinary excretion of thromboxane (Tx)B2 (Geoffroy & al., Hypertension 1986, 4 suppl 3: S37) and an elevated renal sympathetic activity (Sautel & al., Am J Physiol 1988, 255: H736) were simultaneously observed in the developing genetically hypertensive rat of the Lyon strain (LH). In the present work, the relationship between the adrenergic stimulation and prostaglandins (PGs) release was studied using isolated perfused kidney of 8 week-old LH rats and their normotensive controls (LN). Phenylephrine (PHE, 5 - 190 x 10(-8) M) and norepinephrine (NE, 1.2 - 96 x 10(-8) M) were administered in a single pass kidney perfused with a cell free solution and their effects were studied on the renal vascular resistances (RVR) and urinary excretion of TxB2 and 6-keto-PGF1 alpha (6KPGF) measured by specific radioimmunoassays after separation by HPLC. The results (mean +/- SE) obtained before (C) and after PHE and NE perfusions at concentrations: D1 = 31 and 10.5; D2 = 190 and 96 10(-8) M for PHE and NE respectively (* p less than 0.05 ** p less than 0.01 *** p less than 0.001 LH vs LN) were as follows: [table: see text] During the control period, kidneys of LH rats exhibited increased RVR when compared to LN controls but a similar PG excretion. The 2 concentrations of PHE and NE used which produced a similar increase in RVR strikingly stimulated the PG excretion. This effect which was more marked for NE than for PHE did not differ between the 2 strains for 6KPGF but was enhanced for TxB2 in kidney of LH rat.(ABSTRACT TRUNCATED AT 250 WORDS)
Liu
K L
KL
Département de physiologie et pharmacologie clinique, URA-CNRS 606, faculté de pharmacie, Lyon.
Benzoni
D
D
Hadj Aissa
A
A
Lareal
M C
MC
Vincent
M
M
Sassard
J
J
Zech
P Y
PY
D000818:Animals; D006973:Hypertension / Q000235:genetics* / Q000503:physiopathology; D007668:Kidney / Q000378:metabolism* / Q000502:physiology; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D010656:Phenylephrine / Q000494:pharmacology; D011453:Prostaglandins / Q000096:biosynthesis* / Q000652:urine; D051381:Rats; D013564:Sympathetic Nervous System / Q000503:physiopathology; D013930:Thromboxane-A Synthase / Q000378:metabolism; D014655:Vascular Resistance / Q000187:drug effects
D011453:Prostaglandins; D010656:Phenylephrine; D013930:Thromboxane-A Synthase; D009638:Norepinephrine
Stimulation adrénergique et synthèse rénale des prostaglandines chez le rat génétiquement hypertendu de souche lyonnaise.