Regulation of in vivo expression of Fc receptors for IgE (Fc epsilon R) on murine lymphocytes. I. Detection of Fc epsilon R+ lymphocytes by flow cytometry.
Homologous monomeric IgE was employed in a flow cytometric assay for the detection of IgE Fc receptors (Fc epsilon R) on mouse lymphocytes. The expression of Fc epsilon R in normal BALB/c mice was detected on splenic and circulating lymphocytes, but not on bone marrow cells. The Fc epsilon R expression was observed in B cells with B220, surface IgM, and IgD, but not in T cells. Infection of mice with Nippostrongylus brasiliensis resulted in a marked increase in the expression of Fc epsilon R on splenic B cells. T cells, however, did not express Fc epsilon R even after N. brasiliensis infection. On the other hand, the Fc epsilon R expression on normal B cells decreased after a simple incubation at 37 degrees C for 24 h, while in the presence of IgE this decrease was inhibited. In contrast, B cells stimulated with interleukin 4 display Fc epsilon R with high densities. Interestingly, IgE enhanced the Fc epsilon R expression induced by interleukin 4, suggesting that both interleukin 4 and IgE may be responsible for an increase in the expression of Fc epsilon R on B cells of N. brasiliensis infected mice.
Kiniwa
M
M
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Yanagihara
Y
Y
Watanabe
N
N
Tasaka
K
K
D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000096:biosynthesis / Q000378:metabolism*; D000947:Antigens, Helminth / Q000032:analysis; D001402:B-Lymphocytes / Q000378:metabolism*; D015415:Biomarkers / Q000032:analysis; D001853:Bone Marrow; D005434:Flow Cytometry; D007073:Immunoglobulin E / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009349:Nematode Infections / Q000276:immunology; D009559:Nippostrongylus / Q000276:immunology; D011961:Receptors, Fc / Q000096:biosynthesis / Q000378:metabolism*; D017455:Receptors, IgE; D013154:Spleen
D000944:Antigens, Differentiation, B-Lymphocyte; D000947:Antigens, Helminth; D015415:Biomarkers; D011961:Receptors, Fc; D017455:Receptors, IgE; D007073:Immunoglobulin E