Pertussis toxin inhibits the FMLP-induced membrane association of protein kinase C in human neutrophils.
The effect of pertussis toxin on N-formyl-methionyl-leucyl-phenylalanine (FMLP), phorbol 12-myristate 13-acetate (PMA), ionomycin, and A23187-induced translocation of protein kinase C and superoxide generation was studied in human neutrophils. Pertussis toxin was shown to inhibit FMLP-induced superoxide generation in parallel with an inhibition of protein kinase C translocation. In contrast, no effect of pertussis toxin was demonstrated upon superoxide generation or protein kinase C translocation in PMA-, A23187-, or ionomycin-stimulated cells. In that superoxide generation and protein kinase C translocation were inhibited in parallel, it is concluded that a G-protein-dependent cascade is involved in the FMLP-induced activation of protein kinase C, and this cascade may be equivalent to the pathway inducing superoxide generation.
Christiansen
N O
NO
Department of Medicine and Infectious Diseases, Marselisborg Hospital, University of Aarhus, Denmark.
D002462:Cell Membrane / Q000201:enzymology; D019204:GTP-Binding Proteins / Q000502:physiology; D006801:Humans; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology*; D037342:Pertussis Toxin; D011493:Protein Kinase C / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D010566:Virulence Factors, Bordetella; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D037342:Pertussis Toxin; D011493:Protein Kinase C; D019204:GTP-Binding Proteins; D013755:Tetradecanoylphorbol Acetate