Mastoparan, a peptide toxin from wasp venom, stimulates glycogenolysis mediated by an increase of the cytosolic free Ca2+ concentration but not by an increase of cAMP in rat hepatocytes.
A wasp venom, mastoparan, rapidly increased the cytosolic free Ca2+ concentration [( Ca2+]i) and activated phosphorylase in rat hepatocytes in a concentration-dependent manner. Mastoparan could increase [Ca2+]i even in the absence of extracellular Ca2+, but a larger increase was observed in the presence of extracellular Ca2+. Thus, mastoparan mobilized Ca2+ from intracellular and extracellular Ca2+ stores. It also activated inositol triphosphate (IP3) accumulation, but did not stimulate cAMP production. From these results, we conclude that mastoparan activates rat hepatic glycogenolysis mediated by the accumulation of IP3, which causes an increase of [Ca2+]i but not that mediated by cAMP.
Tohkin
M
M
Shionogi Research Laboratories, Shionogi and Co. Ltd., Osaka, Japan.
Yagami
T
T
Matsubara
T
T
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D001514:Bee Venoms / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism*; D003600:Cytosol / Q000187:drug effects* / Q000378:metabolism; D004837:Epinephrine / Q000494:pharmacology; D005947:Glucose / Q000096:biosynthesis; D007295:Inositol Phosphates / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008112:Liver Glycogen / Q000378:metabolism*; D008297:Male; D010455:Peptides; D051381:Rats; D011919:Rats, Inbred Strains; D014862:Wasp Venoms / Q000494:pharmacology*
D001514:Bee Venoms; D007295:Inositol Phosphates; D036341:Intercellular Signaling Peptides and Proteins; D008112:Liver Glycogen; D010455:Peptides; D014862:Wasp Venoms; C037500:mastoparan; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D005947:Glucose; D002118:Calcium; D004837:Epinephrine