[Cloning and sequencing of immunoglobin lambda-chain cDNA in american mink (Mustela vison].
cDNA library in the lambda gt11 phage was constructed using poly (A+)-mRNA from mink spleen as a template. Immunoscreening of the library allowed the identification of 2 lambda-related clones containing 370 and 803 bp insertion (lambda IGL-1 and lambda IGL-2). Analysis of the primary structure of lambda IGL-2 demonstrated that it contains a large portion of V lambda-segment, J lambda-segment, C lambda-gene and its 3'-untranslated part. The nucleotide sequences known for the immunoglobulin genes were compared to the sequence of the lambda IGL-2 clone. The highest degree of homology was established for the rabbit lambda-genes, this being 63, 94 and 72% for the RF3 region of V lambda-segment, J lambda-segment and C lambda-region, respectively.
Naiakshin
A M
AM
Belousov
E S
ES
Taranin
A V
AV
Bogachev
S S
SS
Rogozin
I B
IB
Baranov
O K
OK
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D015151:Immunoblotting; D007146:Immunoglobulin lambda-Chains / Q000235:genetics*; D008907:Mink / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D011817:Rabbits; D012689:Sequence Homology, Nucleic Acid
D007146:Immunoglobulin lambda-Chains; D004247:DNA
Klonirovanie i analiz pervichnoĭ struktury kDNK lambda-tsepi immunoglobulinov amerikanskoĭ norki (Mustela vison).