Nuclear transport of proteins translated in vitro from SP6 plasmid-generated mRNAs.
A sensitive and versatile assay is described for the nuclear transport of 35S-labeled proteins obtained by the in vitro translation of SP6 plasmid-generated mRNAs. A specific nuclear accumulation of greater than 20-fold is observed for the transformation-related nuclear proteins, p53 and E1b, and the nuclear enzyme, thymidine kinase, whereas transport of the nonnuclear proteins, dihydrofolate reductase and simian virus 40 small t antigen, is negligible within 30 min.
Parnaik
V K
VK
Centre for Cellular and Molecular Biology, Hyderabad, India.
Kennady
P K
PK
D000255:Adenosine Triphosphate / Q000378:metabolism; D017456:Adenovirus Early Proteins; D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism*; D002474:Cell-Free System; D003593:Cytoplasm / Q000502:physiology; D004274:DNA, Recombinant; D005455:Fluorescent Antibody Technique; D066298:In Vitro Techniques; D009687:Nuclear Proteins / Q000378:metabolism*; D015513:Oncogene Proteins / Q000378:metabolism; D009856:Oncogene Proteins, Viral / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D010957:Plasmids; D014176:Protein Biosynthesis; D013937:Thymidine Kinase / Q000378:metabolism; D016159:Tumor Suppressor Protein p53
D017456:Adenovirus Early Proteins; D004274:DNA, Recombinant; D009687:Nuclear Proteins; D015513:Oncogene Proteins; D009856:Oncogene Proteins, Viral; D010750:Phosphoproteins; D016159:Tumor Suppressor Protein p53; D000255:Adenosine Triphosphate; D013937:Thymidine Kinase