Murine interstitial nephritis. IX. Induction of the nephritogenic effector T cell repertoire with an antigen-specific T cell cytokine.
The experiments presented in this report describe the biochemical and functional characteristics of a soluble Th cell factor (ThF) which can induce a nephritogenic effector T cell repertoire producing autoimmune interstitial nephritis. The ThF is Ag-specific, I-A-restricted, and comprises two chains noncovalently linked as a heterodimer. One chain at approximately 78,000 Mr is related to the TCR/Id and expresses a framework determinant (14-30) common to Ag-binding factors, and the other chain at approximately 82,000 Mr is I-A+. Together these chains can replace their parent cell by providing cognate help to precursor effector T lymphocytes in the presence of accessory cells, tubular Ag, and IL-2.
Hines
W H
WH
Renal-Electrolyte Section, University of Pennsylvania School of Medicine, Philadelphia 19104.
Mann
R A
RA
Kelly
C J
CJ
Neilson
E G
EG
D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology*; D001485:Basement Membrane / Q000276:immunology; D002846:Chromatography, Affinity; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007684:Kidney Tubules / Q000276:immunology; D008222:Lymphokines / Q000276:immunology* / Q000302:isolation & purification; D046911:Macromolecular Substances; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D009395:Nephritis, Interstitial / Q000276:immunology*; D010449:Peptide Mapping; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D012995:Solubility; D013601:T-Lymphocytes / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D000949:Histocompatibility Antigens Class II; D008222:Lymphokines; D046911:Macromolecular Substances; D011948:Receptors, Antigen, T-Cell