Determination of dimethoate in blood and hemoperfusion cartridge following ingestion of formothion: a case study.
A 57-year-old male who had ingested not more than 22 g of formothion was semicomatose on admission to hospital, approximately 1.5 h after ingestion. Dimethoate, a hydrolyzed formothion, was found in blood samples collected from the patient and in the charcoal column in the direct hemoperfusion cartridge which was used 6 to 7.5 h after ingestion. It was extracted and purified by Extrelut column extraction. A gas chromatograph, equipped with a flame photometric detector and a gas chromatograph-mass spectrometer, were used to detect and confirm the presence of dimethoate. The blood dimethoate concentrations which were taken approximately 1.5 and 6 h after ingestion were 21.4 and 12.7 micrograms/g, respectively. A blood dimethoate concentration of 21.4 micrograms/g would appear to indicate a high level of formothion intoxication. The total amount of dimethoate found in the charcoal column used was 15 mg.
Kojima
T
T
Department of Legal Medicine, Hiroshima University School of Medicine, Japan.
Yashiki
M
M
Ohtani
M
M
Chikasue
F
F
Miyazaki
T
T
D000327:Adsorption; D002138:Calibration; D002849:Chromatography, Gas; D004117:Dimethoate / Q000032:analysis / Q000097:blood*; D005278:Fenitrothion / Q000097:blood; D008401:Gas Chromatography-Mass Spectrometry; D006464:Hemoperfusion; D006801:Humans; D006868:Hydrolysis; D008297:Male; D008875:Middle Aged; D009946:Organothiophosphorus Compounds / Q000097:blood / Q000378:metabolism / Q000506:poisoning*
D009946:Organothiophosphorus Compounds; D004117:Dimethoate; D005278:Fenitrothion