Etiology of intestinal damage in gastroschisis, II. Timing and reversibility of histological changes, mucosal function, and contractility.
Previous work in the fetal lamb examined the relative effects of amniotic fluid and bowel constriction in the etiology of bowel damage in gastroschisis. The present study used the same model to assess the timing and reversibility of these changes during gestation. Gastroschisis was created at 80 days' gestation, and a tape was placed around the bowel to cause gradual constriction with growth. Lambs were killed at 100 days, 120 days, and term. Bowel damage was assessed using histology, mucosal enzyme activity, and in vitro motility. In an additional "repaired" group, the constrictor was removed at 120 days, a silastic pouch placed over the bowel, and bowel damage assessed at term. Normal fetuses at each gestational age were used as controls. A fibrous peel was observed at all gestational ages. Mucosal villous atrophy and mesenteric venous and lymphatic dilation were mild at 100 and 120 days, but severe at term. These changes were present but mild in repaired animals at term. Mucosal enzyme activity decreased gradually with gestational age; inhibition of maltase activity was maximal at term, and was significantly reversed by repair, whereas inhibition of aminooligopeptidase activity was maximal at 120 days, and was not affected by repair. Protein/DNA, DNA/weight, and protein/weight ratios showed that repaired mucosal cells were significantly more proliferative, smaller, and less mature than control or gastroschisis cells. In vitro motility studies demonstrated a mild decrease in contractility at 100 and 120 days, and a large decrease at term. This deleterious effect at the end of gestation was only partially reversed by repair in utero.(ABSTRACT TRUNCATED AT 250 WORDS)
Langer
J C
JC
Fetal Treatment Program, University of California, San Francisco.
Bell
J G
JG
Castillo
R O
RO
Crombleholme
T M
TM
Longaker
M T
MT
Duncan
B W
BW
Bradley
S M
SM
Finkbeiner
W E
WE
Verrier
E D
ED
Harrison
M R
MR
D000009:Abdominal Muscles / Q000002:abnormalities* / Q000473:pathology; D000653:Amniotic Fluid / Q000502:physiology; D000818:Animals; D036861:Delivery, Obstetric; D007410:Intestinal Diseases / Q000209:etiology / Q000473:pathology / Q000503:physiopathology; D007413:Intestinal Mucosa / Q000473:pathology* / Q000503:physiopathology; D007415:Intestinal Obstruction / Q000150:complications; D009119:Muscle Contraction; D012756:Sheep; D013997:Time Factors