[Preparation, identification and functional studies on liposome (ApoA-I:PC)].
Liposome (ApoA-1: PC) was prepared by the deoxycholic acid dialysis method and identified by agarose gel electrophoresis, two dimensional thin-layer chromatography and electron microscopy. The purified liposomes manifested one band in Amino black 10B staining and no color in Oil red O staining due to the presence of phospholipid. Only one brown spot appeared in two dimensional TLC, and electron microscopy with 1% sodium phosphotungstate negative staining revealed discoidal shapes stuck together. All of the above indicate that the liposome consisted of ApoA-1 and PC. Liposomes were added to the medium of smooth muscle cell cultures and then LDL receptor expression and cholesterol efflux from the cells were observed. The results suggest that the ApoA-1:PC can enhance the expression of LDL receptors indirectly by stimulating cholesterol efflux from the cells.
Hu
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Y
Institute of Basic Medical Sciences, Beijing.
D000818:Animals; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism; D004337:Drug Carriers; D008075:Lipoproteins, HDL; D008081:Liposomes; D009130:Muscle, Smooth / Q000166:cytology; D010713:Phosphatidylcholines / Q000008:administration & dosage / Q000494:pharmacology; D011817:Rabbits; D011973:Receptors, LDL / Q000032:analysis
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D004337:Drug Carriers; D008075:Lipoproteins, HDL; D008081:Liposomes; D010713:Phosphatidylcholines; D011973:Receptors, LDL; D002784:Cholesterol