Transmission of enteric non-A, non-B hepatitis virus in Macaca mulatta monkeys by intraportal route: subsequent passages of HEV virus.
Macaca mulatta monkeys have been used for the transmission of enteric non-A, non-B hepatitis (HEV) virus by intraportal route. Subsequent passages of HEV virus have been completed in these monkeys. In the first passage, 2 monkeys were inoculated by intra-portal route with 27-34 nm virus-like particles (VLP) obtained from known epidemics of HEV hepatitis in India, and biochemical and serological changes in the blood, histological changes in the liver and excretion of 27-34 nm VLP in the stool were studied. Results were compared with those of 4 negative control monkeys inoculated with stool extracts from healthy individuals. The second passage of 27-34 nm VLP was carried out on 2 monkeys using pools of stool suspension positive for 27-34 nm VLP from first passaged animals. Similarly, the third passage of 27-34 nm VLP was completed intraportally in another monkey. All monkeys developed acute hepatitis, as evidenced by transient elevation of aminotransferase, histopathological changes in the liver, development of antibodies aggregating 27-34 nm VLP and excretion of 27-34 nm VLP in stools. No control monkeys developed these features.
Gupta
H
H
Department of Gastroenterology & Human Nutrition, All India Institute of Medical Sciences, New Delhi.
Joshi
Y K
YK
Varma
A
A
Shenoy
S
S
Sriramchari
S
S
Iyenger
B
B
Tandon
B N
BN
D000818:Animals; D005243:Feces / Q000382:microbiology; D016751:Hepatitis E / Q000473:pathology / Q000635:transmission*; D016752:Hepatitis E virus; D006801:Humans; D007275:Injections, Intravenous; D008099:Liver / Q000382:microbiology / Q000473:pathology; D008253:Macaca mulatta; D016253:Microscopy, Immunoelectron; D011169:Portal Vein; D014771:Virion