Use of detergents in the preparation of Salmonella samples for enzyme immunoassay on polymyxin-coated polyester cloth.
Heating Salmonella cells in the presence of detergents such as deoxycholate or Triton x-100 greatly increased the sensitivity of an enzyme immunoassay for lipopolysaccharide antigens using polymyxin-coated polyester cloth as the solid phase for antigen capture. The presence of a 100-fold excess of E. coli lipopolysaccharide in the test sample did not affect the detection of the Salmonella lipopolysaccharide. The sensitivity of the assay using deoxycholate-heat treatment of the antigens followed by detection on polymyxin-coated cloth was equivalent to that of an assay using antibody-coated polyester cloth for antigen capture.
Blais
B W
BW
Department of Biology, Carleton University, Ottawa, Canada.
Yamazaki
H
H
D003840:Deoxycholic Acid; D003902:Detergents; D004492:Edetic Acid; D006358:Hot Temperature; D007124:Immunoenzyme Techniques; D007700:Kinetics; D008070:Lipopolysaccharides / Q000032:analysis*; D017830:Octoxynol; D011091:Polyesters; D011092:Polyethylene Glycols; D011112:Polymyxin B; D011136:Polysorbates; D011237:Predictive Value of Tests; D012486:Salmonella typhimurium / Q000276:immunology*
D003902:Detergents; D008070:Lipopolysaccharides; D011091:Polyesters; D011136:Polysorbates; D003840:Deoxycholic Acid; D011092:Polyethylene Glycols; D017830:Octoxynol; D004492:Edetic Acid; D011112:Polymyxin B