Co-immobilization and interaction of heparin and plasmin on collageno-elastic tubes.
Heparin and plasmin were co-immobilized on collageno-elastic tubes (CETs) in order to develop a thromboresistant and fibrinolytic vascular prosthesis. The mutual interaction between heparin and plasmin both in the soluble and in the immobilized state was studied. The immobilization condition rendering maximum co-immobilized heparin and plasmin activity was identified to require heparin immobilization followed by plasmin immobilization. Soluble heparin exerts a positive synergistic effect on soluble plasmin. Immobilized heparin enhances plasmin loading on the CET as compared to the heparin-free graft. Heparin, in both the soluble or immobilized state, significantly decreases the Michaelis-Menten (M-M) parameter Km for immobilized plasmin over heparin-free immobilized plasmin. Furthermore, the M-M parameter Vmax for the immobilized plasmin in the presence of heparin decreases over heparin-free immobilized plasmin. These results suggest a decrease in the kinetic constant k3 for heparin-modified immobilized plasmin over the heparin-free form. Co-immobilized heparin-plasmin collagenous grafts represent a unique advance in the development of fibrinolytically active prostheses.
Shankar
H
H
Department of Chemical Engineering, Texas Tech University, Lubbock 79409.
Senatore
F
F
Wu
D R
DR
Avantsa
S
S
D000818:Animals; D003094:Collagen; D005341:Fibrinolysin / Q000378:metabolism*; D005342:Fibrinolysis; D005971:Glutamates / Q000378:metabolism; D006493:Heparin / Q000378:metabolism*; D012756:Sheep; D013552:Swine
D005971:Glutamates; D006493:Heparin; D003094:Collagen; D005341:Fibrinolysin