Comparative studies on semicarbazide-sensitive amine oxidase in heart and plasma of rats treated with hepatotoxin allyl formate.
1. After allyl formate (AF) was administered to the rats, the existence of semicarbazide-sensitive amine oxidase (SSAO) in rat identified. 2. When the heart homogenate and plasma of AF-administered rat were pretreated with 10(-3) M clorgyline and deprenyl, the Km value for benzylamine of rat heart was same as the value of plasma. 3. The existence of SSAO in plasma of AF-administered rats were identified by IEF-gel electrophoresis. The pI values of SSAO in heart and plasma were a single peak of 5.0. 4. SSAO released from the rat heart in response to AF, although the other origins of this enzyme are unknown.
Obata
T
T
Department of Pharmacology, Medical College of Oita, Japan.
Yamanaka
Y
Y
D000818:Animals; D003010:Clorgyline / Q000494:pharmacology; D005563:Formic Acid Esters / Q000494:pharmacology*; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D008297:Male; D008995:Monoamine Oxidase / Q000097:blood / Q000378:metabolism*; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology*; D009206:Myocardium / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D012642:Selegiline / Q000494:pharmacology; D012663:Semicarbazides / Q000494:pharmacology*
D005563:Formic Acid Esters; D008996:Monoamine Oxidase Inhibitors; D012663:Semicarbazides; D012642:Selegiline; C010059:carbamylhydrazine; C034499:allyl formate; D008995:Monoamine Oxidase; D003010:Clorgyline