title: Proliferation of HIV in lymphocyte--associated macrophages with cytopathic changes in AIDS erythroderma.
abstract: Latency of the Human Immunodeficiency Virus (HIV) has been demonstrated in both helper T-Lymphocytes and cells of the macrophage/monocyte series. Although mitogen-dependent amplification of HIV infection within lymphocytes and monocytes/macrophages has been demonstrated to occur in vitro, in vivo evidence of such a phenomenon has been lacking. We have performed electron microscopic and immunocytochemical evaluation of skin biopsies from a patient with the Acquired Immunodeficiency Syndrome (AIDS) with chronic erythroderma. These biopsies provided evidence of proliferation of HIV in macrophages interacting with activated lymphocytes (CD3+). These macrophages were undergoing morphologic changes characteristic of cytopathicity and contained numerous viral particles, many of which were actively budding from plasma membranes. Cutaneous macrophages which were not interacting with lymphocytes did not demonstrate cytopathicity or evidence of viral multiplication. These in vivo data substantiate the concept that activation of cells which harbour latent HIV promotes viral replication as well as subsequent cytopathicity.
authors: Division of Dermatology, UCLA-San Fernando Valley International Medicine Program, VA Medical Center, Sepulveda 91343.
authors: M C
authors: MC
authors: Heng
authors: M
authors: M
authors: Lambertus
authors: M B
authors: MB
authors: Goetz
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology / Q000473:pathology*; D001706:Biopsy; D002455:Cell Division; D003873:Dermatitis, Exfoliative / Q000382:microbiology / Q000473:pathology*; D006678:HIV / Q000648:ultrastructure*; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology* / Q000648:ultrastructure; D008264:Macrophages / Q000382:microbiology* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D012867:Skin / Q000648:ultrastructure