Identification of the type-B receptor for platelet-derived growth factor in human embryonal carcinoma cells.
The human embryonal carcinoma (EC) cell line Tera 2 clone 13 (T2/13) can be induced to differentiate in vitro into neuroectodermal cell types with retinoic acid. Undifferentiated cells are characterized by rapid proliferation, whereas differentiated cells show a prolonged generation time, have a limited life span, and possess new cell-surface markers. In the present study we establish that both differentiated and undifferentiated T2/13 cells express the type-B platelet-derived growth factor (PDGF) receptor mRNA and bind PDGF-BB with high affinity. Differentiation causes a three-fold increase in receptor number per cell and leaves the affinity of the receptors unaffected. These data are the first to describe expression of this receptor in EC cells. The biosynthesis and degradation of this receptor were studied in undifferentiated as well as in differentiated T2/13 cells using an anti-type-B receptor antibody. These experiments revealed that high concentrations of recombinant PDGF-AA did not accelerate receptor metabolism in both cell types. In contrast, human PDGF or recombinant PDGF-BB added to the culture dishes readily increased receptor degradation. These results demonstrate that T2/13 cells express functional type-B PDGF receptors and suggest that cells responsive to PDGF might be present during mammalian development before the onset of mesoderm formation.
Weima
S M
SM
Hubreht Laboratory, Netherlands Institute for Developmental Biology, The Netherlands.
van Rooijen
M A
MA
Mummery
C L
CL
Feyen
A
A
de Laat
S W
SW
van Zoelen
E J
EJ
D002454:Cell Differentiation / Q000187:drug effects; D054278:Embryonal Carcinoma Stem Cells; D015870:Gene Expression; D006801:Humans; D007106:Immune Sera; D007163:Immunosorbent Techniques; D046911:Macromolecular Substances; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D017479:Receptors, Platelet-Derived Growth Factor; D011994:Recombinant Proteins; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D007106:Immune Sera; D046911:Macromolecular Substances; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D014212:Tretinoin; D017479:Receptors, Platelet-Derived Growth Factor