title: Glycerol facilitator of Escherichia coli: cloning of glpF and identification of the glpF product.
abstract: The glycerol facilitator is known as the only example of a transport protein that catalyzes facilitated diffusion across the Escherichia coli inner membrane. Here we show that the gene encoding the facilitator, glpF, is the first gene in an operon with glpK, encoding glycerol kinase, at 88 min of the E. coli chromosome. The operon is transcribed counterclockwise. We cloned the glpF gene, demonstrated that it complemented a chromosomal glycerol transport-minus mutation, and identified the gene product. The GlpF protein appeared in the membrane fraction of plasmid-bearing strains and had an apparent Mr of 25,000.
authors: Department of Biology, University of Konstanz, Federal Republic of Germany.
authors: G
authors: G
authors: Sweet
authors: C
authors: C
authors: Gandor
authors: R
authors: R
authors: Voegele
authors: N
authors: N
authors: Wittekindt
authors: J
authors: J
authors: Beuerle
authors: V
authors: V
authors: Truniger
authors: E C
authors: EC
authors: Lin
authors: W
authors: W
authors: Boos
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D005991:Glycerol Kinase / Q000235:genetics*; D009154:Mutation; D009876:Operon; D010770:Phosphotransferases / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D010770:Phosphotransferases; D005991:Glycerol Kinase; D005990:Glycerol