Preparation and characterization of anti-paroxetine antibodies.
6-nitroparoxetine was synthesized and reduced to 6-aminoparoxetine. After coupling to glutaraldehyde at the 6-position and to bovine serum albumin, the resulting Schiff's base was further reduced into an amino-derivative which served as the antigen. Anti-paroxetine antibodies were raised against this antigen in rabbits and the anti-paroxetine IgG purified by Protein A affinity chromatography. The anti-paroxetine IgG demonstrated high specificity towards paroxetine and 6-nitroparoxetine without significant cross-reactivity with other commonly used antidepressant and neuroleptic drugs. These antibodies may be useful for both plasma paroxetine level assays and uptake inhibitor binding site studies.
Strijewski
A
A
Psychopharmacology Unit, Clarke Institute of Psychiatry, Toronto, Canada.
Tang
S W
SW
D000818:Animals; D000915:Antibody Affinity; D000918:Antibody Specificity; D003429:Cross Reactions; D005976:Glutaral; D006241:Haptens / Q000032:analysis; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000302:isolation & purification; D017374:Paroxetine; D010880:Piperidines / Q000032:analysis / Q000276:immunology*; D011817:Rabbits; D011863:Radioimmunoassay; D012702:Serotonin Antagonists / Q000032:analysis / Q000276:immunology*; D012710:Serum Albumin, Bovine
D006241:Haptens; D007074:Immunoglobulin G; D010880:Piperidines; D012702:Serotonin Antagonists; D012710:Serum Albumin, Bovine; D017374:Paroxetine; D005976:Glutaral