Four tightly linked rab genes are differentially expressed in rice.
We have cloned and sequenced the four members of a rice rab (responsive to abscisic acid) gene family that are tandemly arrayed in a locus approximately 30 kbp in length. Each of the genes contains a single, small intron. They are all transcriptionally active and encode proteins of Mr 15,500-16,800 with two highly conserved domains. Northern analysis with gene-specific probes showed slightly different patterns of expression for the four genes in rice plant tissues and in response to osmotic stress. Comparison of the promoter regions revealed a conserved GC-rich sequence (CGG/CCGCGCT) with some homology to the SP1 binding site (Briggs et al., 1986). Another conserved sequence (PuTACGTGGCPu), whose core is found in the promoter regions of ABA-responsive cotton genes, is reminiscent of the cAMP responsive element (Deutsch et al., 1988).
Yamaguchi-Shinozaki
K
K
Laboratory of Plant Molecular Biology, Rockefeller University, New York, NY 10021.
Mundy
J
J
Chua
N H
NH
D000040:Abscisic Acid / Q000502:physiology; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D008040:Genetic Linkage; D007438:Introns; D008969:Molecular Sequence Data; D005810:Multigene Family; D015345:Oligonucleotide Probes; D012275:Oryza / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D015345:Oligonucleotide Probes; D010940:Plant Proteins; D000040:Abscisic Acid