title: [Determination of enoximone and its principle metabolite in serum and urine using high pressure liquid chromatography].
abstract: We developed a high performance liquid chromatography method for the monitoring of enoximone and its main metabolite in serum and urine. Samples handling involves a unique chemical extraction step by ethylacetate. Serum needs at first to be deproteinized by acetonitril. The chromatographic separation is realized on a reversed phase analytical column by gradient elution with acetonitrile. Quantification is by U.V. absorbance at 365 nm. We compared our new method with the method so far considered as reference one. When specificity, accuracy and linearity of both procedures are similar, we greatly enhanced the detection limit [(5 ng/ml for (E) and (SE)] and the practicability: ease of use, rapidity and lower cost.
authors: Institut de Bactériologie, Faculté de Médecine, Strasbourg.
authors: E
authors: E
authors: Tarral
authors: F
authors: F
authors: Jehl
authors: C
authors: C
authors: Gallion
authors: H
authors: H
authors: Monteil
mesh_terms: D002316:Cardiotonic Agents / Q000032:analysis* / Q000097:blood / Q000378:metabolism / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017335:Enoximone; D006801:Humans; D007093:Imidazoles / Q000032:analysis* / Q000097:blood / Q000378:metabolism / Q000652:urine
chemical_list: D002316:Cardiotonic Agents; D007093:Imidazoles; D017335:Enoximone
vernacular_title: Dosage de l'enoximone et de son principal métabolite dans le sérum et l'urine par chromatographie liquide à haute performance.