Omental dendritic cells: Ia expression and relation to macrophages.
Rat omental dendritic cells (ODC) occur in two forms, mainly spindle-shaped, Ia-, on the surface, and stellar, Ia+ within the omentum. Like macrophages, most ODC label with W3/25 mAb and have a demonstrable activity of non-specific esterase, acid phosphatase and ATPase. Up to 30% of ODC are actively phagocytic. The proportion of Ia+ ODC rises 7 days after ip antigenic stimulation and there seems to be a reciprocal development of Ia positivity and phagocytic capacity between ODC and macrophages 3 and 5 days after ip immunization. ODC contribute to the overall fluctuation of Ia expression in the entire omentum after immunization. Ia+ ODC display a linear arrangement along preilymphatic spaces and may be related to the formation of new lymph vessels. They also constitute the stroma of pseudofollicles containing clusters of what are presumably T-helper lymphocytes closely attached to ODC, scattered suppressor T lymphocytes and B cells accumulating in later stages at the immune response.
Holub
M
M
Institute for Clinical and Experimental Medicine, Czechoslovak Academy of Science, Prague.
Vĕtvicka
V
V
Ríha
I
I
Svobodová
J
J
Mohelská
H
H
D000135:Acid Phosphatase / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D004950:Esterases / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D009852:Omentum; D051381:Rats; D011919:Rats, Inbred Strains; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure
D000949:Histocompatibility Antigens Class II; D004950:Esterases; D000135:Acid Phosphatase; D000251:Adenosine Triphosphatases