[Determination of cytoplasmic glycogen in 4 strains of amoeba of the genus Entamoeba].
The purpose of this work was made to determine the cytoplasmic glycogen concentration in trophozoites from four different strains of Entamoeba genus. We used IP-1 and PZ from E. invadens; HM-2 and HK-9 from E. histolytica strains, axenically cultured in TP-S-1 medium. The growth of different strains was determined each 24 hours during 10-days, with the aid of a hemocytometer. The quantification of cytoplasmic glycogen was made by two methods: 1) Montgomery and 2) Krisman. The glycogen concentrations were measured in the four strains during their growing. The results did not show any difference, remained as a constant concentration in each strain. These concentrations quantified by Montgomery method were similar in all strains (35 pg/cell), except in HM-2 strain which showed a less concentration of this polysaccharide (28 pg/cell) than the other strain. These results were different when Krisman method was used, both IP-1 and PZ strains had a similar glycogen concentrations about 19 pg/cell, however HM-2 and HK-9 strains had a higher glycogen concentration (21 pg/cell) than E. invadens strains.
Segura
J J
JJ
Subjefatura de Investigación Científica, Unidad de Investígación Biomédica del Noreste, IMSS, Monterrey, N.L., México.
Calzado-Flores
C
C
Martínez-Moncivais
J
J
D000704:Analysis of Variance; D000818:Animals; D003593:Cytoplasm / Q000737:chemistry; D004747:Entamoeba / Q000737:chemistry*; D004748:Entamoeba histolytica / Q000737:chemistry; D006003:Glycogen / Q000032:analysis*; D013045:Species Specificity
D006003:Glycogen
Determinación de glucógeno citoplasmático en cuatro cepas de amibas del género Entamoeba.