title: Different isoforms of human FcRII distinguished by CDw32 antibodies.
abstract: The Third and Fourth International Workshops on Leucocyte Differentiation Antigens identified six mAb, designated CDw32, reacting with human Ig FcR type II (FcRII). We have examined the immunohistochemical and immunocytologic reactivities of these antibodies and find that the antibodies could be divided into three classes of reactivity: 1) antibodies IV.3, CIKM3, and CIKM5 reacted with monocytes, macrophages and neutrophils; 2) antibodies KB61 and 41H.16 gave strong reactions with B lymphocytes, placental and hepatic endothelium, and weaker reactions with monocytes, macrophages, and neutrophils; 3) antibody 2E1 gave an intermediate reaction pattern. Immunoprecipitation from U937 cell lysates showed that antibodies KB61 and 41H.16 recognized Mr 41,000 and Mr 37,000 molecules whereas the other antibodies detected a Mr 42,000 molecule. Preclearing with antibody KB61 removed the Ag recognized by the other five antibodies confirming the identity of the Ag and demonstrating reactivity of KB61 with the Mr 42,000 molecule. Antibodies KB61 and 41H.16 precipitated a Mr 41,000 molecule from B lymphocytes. Flow cytometry and immunoprecipitation studies of cells transfected with cDNA clones coding for two isoforms of FcRII showed that all six of the antibodies react with both transfectants but the only immunoprecipitations were obtained using KB61 and 41H.16 and one of the transfectants. The protein sequence of KB61 Ag isolated from leukemic B cells showed close homology with the proteins encoded by the cDNA clones but diverged in the intracytoplasmic carboxyl-terminal region. It was concluded that preferential recognition of one or more of the numerous isoforms of FcRII underlies the differing reaction patterns of CDw32 antibodies.
authors: Nuffield Department of Pathology, John Radcliffe Hospital, Headington, Oxford, U.K.
authors: K J
authors: KJ
authors: Micklem
authors: W P
authors: WP
authors: Stross
authors: A C
authors: AC
authors: Willis
authors: J L
authors: JL
authors: Cordell
authors: M
authors: M
authors: Jones
authors: D Y
authors: DY
authors: Mason
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology*; D000943:Antigens, Differentiation / Q000145:classification / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D004727:Endothelium / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D007728:Kupffer Cells / Q000276:immunology; D007801:Langerhans Cells / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010950:Plasma Cells / Q000276:immunology; D011233:Precipitin Tests; D011961:Receptors, Fc / Q000145:classification / Q000276:immunology*; D017452:Receptors, IgG; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; D011961:Receptors, Fc; D017452:Receptors, IgG