Inhibitor of poly(ADP-ribose)transferase potentiates the recombinogenic but not the mutagenic action of alkylating agents in somatic cells in vivo in Drosophila melanogaster.
The effect on somatic mutations and recombination by 3-aminobenzamide (3-ABA), an inhibitor of poly(ADP-ribose) transferase, was studied in the somatic wing spot test in Drosophila melanogaster, 3-ABA did not exert any effect by itself, but caused a strong increase of wing spots in combination with methyl methanesulphonate (MMS) and ethylnitrosourea (ENU). MMS and ENU differ in their mutagenic effects, ENU causing a much higher frequency of point mutations than MMS. The enhancement by 3-ABA of chemically induced wing spots was totally eliminated by the introduction of a heterozygous inversion. It can be concluded that 3-ABA interacted with the chemical mutagens for the induction of somatic recombination, but had no effect on point mutations or intragenic events.
Magnusson
J
J
Department of Genetic and Cellular Toxicology, Wallenberg Laboratory, University of Stockholm, Sweden.
Ramel
C
C
D000477:Alkylating Agents / Q000633:toxicity*; D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004331:Drosophila melanogaster; D005038:Ethylnitrosourea / Q000633:toxicity; D006579:Heterozygote; D008741:Methyl Methanesulfonate / Q000633:toxicity; D009154:Mutation; D010641:Phenotype; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011995:Recombination, Genetic
D000477:Alkylating Agents; D001549:Benzamides; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; C025160:3-aminobenzamide; D008741:Methyl Methanesulfonate; D005038:Ethylnitrosourea