Alterations induced in normal human skin by in vivo interferon-gamma.
In a study of the direct effects of interferon-gamma (IFN-gamma) on normal human skin, healthy adult male volunteers received either 3 micrograms (n = 4) or 30 micrograms (n = 9) of recombinant IFN-gamma administered intradermally over 3 days. Biopsies were taken on day 6 and histopathological examination of fixed paraffin-embedded sections from sites which had received 30 micrograms IFN-gamma revealed a moderate perivascular lymphohistiocytic dermal infiltrate with mast cells. Immunophenotyping of 5 microns cryostat sections demonstrated that 3 micrograms IFN-gamma induced keratinocyte HLA-DR expression in the absence of any significant infiltrate. More intense keratinocyte HLA-DR expression was produced by 30 micrograms IFN-gamma in all specimens, with HLA-DP concurrently expressed in three biopsies. The ratio of CD4:CD8 cells within the infiltrate was approximately 3:1. CD1 + cells within the epidermis were markedly depleted by 30 micrograms IFN-gamma, while CD1-labelled cells were observed in the dermal perivascular infiltrate. Intradermal IFN-gamma induces similar immunopathological changes to those observed in many of the inflammatory dermatoses.
Barker
J N
JN
Laboratory of Applied Dermatopathology, United Medical School, Guy's Hospital, London, U.K.
Allen
M H
MH
MacDonald
D M
DM
D000293:Adolescent; D000328:Adult; D006684:HLA-DR Antigens / Q000032:analysis; D006644:Histiocytes / Q000473:pathology; D006651:Histocytochemistry; D006801:Humans; D007271:Injections, Intradermal; D007371:Interferon-gamma / Q000494:pharmacology*; D015603:Keratinocytes / Q000032:analysis / Q000473:pathology; D008214:Lymphocytes / Q000473:pathology; D008297:Male; D008407:Mast Cells / Q000473:pathology; D011994:Recombinant Proteins; D012867:Skin / Q000187:drug effects* / Q000473:pathology; D006377:T-Lymphocytes, Helper-Inducer / Q000473:pathology
D006684:HLA-DR Antigens; D011994:Recombinant Proteins; D007371:Interferon-gamma