Molasses induced changes in Saccharomyces cerevisiae: alterations in plasma membrane structure and function and metallothionein level.
Growth of yeast cells in molasses caused changes in plasma membrane properties. The polypeptide profile of plasma membrane prepared by sucrose density gradient centrifugation showed a range of new polypeptides above 55 kD which were not present in malt extract-glucose-yeast extract-peptone medium grown cells. Isolated new bands appeared in the range of 25-50 kD. Transport of glucose was markedly enhanced in molasses trained cells. Among the amino acids, L-lysine uptake was decreased while L-aspartate uptake was enhanced considerably. Finally the metallothionein levels in the cells grown in molasses was found to be increased significantly, possibly to protect the cells from the harmful effect of heavy metal ions.
Bhatnagar
N B
NB
Institute of Microbial Technology, Chandigarh, India.
D000409:Alanine / Q000378:metabolism; D001224:Aspartic Acid / Q000378:metabolism; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008239:Lysine / Q000378:metabolism; D008668:Metallothionein / Q000378:metabolism*; D008965:Molasses; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure
D003470:Culture Media; D001224:Aspartic Acid; D008668:Metallothionein; D008239:Lysine; D000409:Alanine