title: Studies of vasoactive intestinal polypeptide expression in injured peripheral neurons using capsaicin, sympathectomy and mf mutant rats.
abstract: The increased expression of vasoactive intestinal polypeptide (VIP) in injured peripheral neurons was studied. In contrast to substance P, there was a marked increase, and maintained fast axonal transport, of VIP in rat sciatic nerve after peripheral axotomy. Local capsaicin application to the nerve trunk failed to inhibit the injury-induced VIP increase, and capsaicin even increased VIP levels when applied locally to uninjured nerves. Pharmacological sympathectomy showed that some of the peripheral VIP increase may occur in post-ganglionic sympathetic fibres. The VIP increase after injury appeared unaffected in the mf mutant rat, in spite of its loss of lumbar dorsal root ganglion cells. VIP-staining fibres in the epi- and peri-neurium and perivascular plexuses of sciatic nerve showed an increase in number in parallel with the changes of the nerve VIP content. These findings suggest that sensory and sympathetic nerve fibres expressing VIP after injury play a role in the regulation of blood flow to nerves, and in the pathophysiological processes in nerve and dorsal spinal cord which follow peripheral nerve injury.
authors: Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, U.K.
authors: P
authors: P
authors: Anand
authors: S J
authors: SJ
authors: Gibson
authors: F
authors: F
authors: Scaravilli
authors: M A
authors: MA
authors: Blank
authors: G P
authors: GP
authors: McGregor
authors: O
authors: O
authors: Appenzeller
authors: K
authors: K
authors: Dhital
authors: J M
authors: JM
authors: Polak
authors: S R
authors: SR
authors: Bloom
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D002211:Capsaicin / Q000494:pharmacology*; D015870:Gene Expression; D006892:Hydroxydopamines; D007150:Immunohistochemistry; D009046:Motor Neurons / Q000187:drug effects; D009474:Neurons / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism; D016627:Oxidopamine; D010525:Peripheral Nerves / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011922:Rats, Mutant Strains; D012584:Sciatic Nerve / Q000166:cytology / Q000187:drug effects; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism; D013563:Sympathectomy, Chemical; D014660:Vasoactive Intestinal Peptide / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D006892:Hydroxydopamines; D009479:Neuropeptides; D014660:Vasoactive Intestinal Peptide; D016627:Oxidopamine; D002211:Capsaicin