Effect of a cell differentiation inducer, ganglioside GM3, on the neutral glycosphingolipid composition and cell membrane fluidity of a human promyelocytic leukemia cell line HL-60.
In this paper the authors confirmed the finding reported by Nojiri et al that exogenous ganglioside GM3 could induce HL-60 cells to differentiate along the monocytic-macrophage route with significant inhibition of proliferation. It was found that after differentiation the neutral GSL content of CDH and CMH increased, while that of CXH, a polyhexosyl ceramide with a TLC mobility slower than CQH, decreased significantly. The membrane fluidity of HL-60 cells decreased after GM3 treatment.
Tsui
Z C
ZC
Department of Biochemistry, Dalian Medical College, P.R. China.
Hou
W H
WH
Yang
L
L
Zhu
Z M
ZM
D002454:Cell Differentiation / Q000187:drug effects*; D005679:G(M3) Ganglioside / Q000494:pharmacology*; D006028:Glycosphingolipids / Q000378:metabolism*; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008560:Membrane Fluidity / Q000187:drug effects*; D008563:Membrane Lipids / Q000096:biosynthesis*; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D013268:Stimulation, Chemical; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D005679:G(M3) Ganglioside; D006028:Glycosphingolipids; D008563:Membrane Lipids