5-Bromodeoxyuridine enhancement of Inoue-Melnick virus synthesis in human colon carcinoma cell lines.
Inoue-Melnick virus (IMV) synthesis in naturally infected human colon carcinoma cell lines can be enhanced by 5-bromodeoxyuridine (BUDR). The optimal conditions for BUDR enhancement differed between two cell lines, SW-480 and DLD-1. SW-480 cells produced 100 times more infectious IMV than DLD-1 cells by BUDR treatment. A clonal difference in IMV synthesis in SW-480 cells was also observed. The highest titer of virus was found in the culture fluid of clone H (SW-480/H) cells treated with BUDR, and a herpes-like virus was detected by negative-staining electron microscopy of concentrates of the culture fluid. One nonproducer clone (SW-480/B) was also activated to produce virus when treated with BUDR.
Inoue
Y K
YK
Institute for Virus Research, Kyoto University, Japan.
Nishibe
Y
Y
Kimura
T
T
D000230:Adenocarcinoma / Q000188:drug therapy / Q000382:microbiology* / Q000473:pathology; D001973:Bromodeoxyuridine / Q000627:therapeutic use*; D003110:Colonic Neoplasms / Q000382:microbiology* / Q000473:pathology; D006801:Humans; D014407:Tumor Cells, Cultured; D014779:Virus Replication; D014780:Viruses / Q000187:drug effects* / Q000254:growth & development
D001973:Bromodeoxyuridine