The construction of a non-antibiotic resistant ETEC vaccine strain containing K88ac and LT-B antigens.
By inserting the LacZ gene into the plasmid containing ETEC LT-B antigen gene we obtained a recombinant plasmid which could express the beta-galactosidase and LT-B antigen, but has the Tc resistant gene. The K88ac antigen gene was then inserted into the BamHI site of the Tc resistant region in the above plasmid. The final recombinant plasmid has no antibiotic resistance gene but can stably express both LT-B and K88ac antigens, as well as the beta-galactosidase as a tracking marker.
Huang
P T
PT
Molecular Genetic Center, Institute of Biotechnology, Beijing.
Cheng
J
J
Li
F S
FS
Xu
X A
XA
Zhang
Q W
QW
Chen
T M
TM
Huang
C F
CF
D000942:Antigens, Bacterial / Q000096:biosynthesis; D000954:Antigens, Surface / Q000096:biosynthesis; D001427:Bacterial Toxins / Q000276:immunology*; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004351:Drug Resistance / Q000235:genetics* / Q000276:immunology; D004768:Enterotoxins / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology*; D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins; D010957:Plasmids; D014170:Transformation, Genetic; D001616:beta-Galactosidase / Q000096:biosynthesis
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C026888:K88 antigen, E coli; D036781:Fimbriae Proteins; C027538:heat-labile enterotoxin, E coli; D001616:beta-Galactosidase