title: Inhibition and recovery of mammalian respiratory ciliary function after formaldehyde exposure.
abstract: Formaldehyde (HCHO) has been reported to impair mucociliary clearance. The present investigation using rabbit and porcine tracheal explants in vitro examined (1) the impairment of ciliary activity, an essential component of mucociliary clearance; (2) the reversibility of ciliary dysfunction after HCHO exposure; and (3) the mechanism by which ciliary activity is reduced by HCHO. HCHO treatment of rabbit tracheal rings significantly decreased zones of active ciliated epithelium in direct proportion to concentration and exposure duration. There was also a significant concentration-dependent reduction of ciliary beat frequency. Removal of HCHO permitted recovery of zones of ciliary activity to normal beat frequencies; greater inhibitory concentrations of HCHO required greater time for return of function. Treatment of porcine tracheae with increasing concentrations of HCHO for time periods inhibitory to rabbit ciliary activity correspondingly reduced the yield of cilia extractable from treated epithelium. Furthermore, the specific activity of ATPase of extracted ciliary axonemes was diminished with increasing HCHO concentration, indicating loss of function. A recovery period following identical exposures of the porcine tracheae to the lower HCHO concentrations resulted in normal yields of functionally intact ciliary axonemes. Similarly, a recovery period after the highest HCHO concentration produced more functional axonemes than obtained from exposed tracheae without a recovery period, although less than normal yields. Therefore, ciliary dysfunction elicited by a defined range of HCHO concentrations is reversible. The yield and functional integrity of ciliary axonemes from epithelium exposed to HCHO with a recovery period are significantly greater than those without such a recovery period, suggesting an alteration and subsequent repair of epithelial surface components following HCHO exposure.
authors: Department of Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
authors: A T
authors: AT
authors: Hastie
authors: H
authors: H
authors: Patrick
authors: J E
authors: JE
authors: Fish
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002923:Cilia / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D004398:Dyneins / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005557:Formaldehyde / Q000008:administration & dosage / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D011817:Rabbits; D013552:Swine; D014132:Trachea / Q000648:ultrastructure*; D014404:Tubulin / Q000378:metabolism
chemical_list: D014404:Tubulin; D005557:Formaldehyde; D000251:Adenosine Triphosphatases; D004398:Dyneins