Recent studies on the molecular biology and biochemistry of CO2 fixation in phototrophic bacteria.
Rhodobacter sphaeroides was found to contain two clusters of chromosomally encoded CO2 fixation structural genes. Recent studies indicate that genes within each cluster are cotranscribed, suggesting that there is a single long transcript for each cluster. All of the genes have been sequenced, homologies noted, specific mutations obtained, and interesting upstream regulatory sequences found. Site-directed mutagenesis studies of the Anacystis rbcS has begun to provide information relative to RubisCO structure and function. In addition, RubisCO-negative strains of photosynthetic bacteria have been constructed to screen for altered RubisCO sequences.
Tabita
F R
FR
Department of Microbiology, Ohio State University, Columbus 43210.
Gibson
J L
JL
Falcone
D L
DL
Lee
B G
BG
Chen
J H
JH
D001483:Base Sequence; D002245:Carbon Dioxide / Q000378:metabolism*; D004798:Enzymes / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D016297:Mutagenesis, Site-Directed; D012242:Rhodobacter sphaeroides / Q000235:genetics*; D013329:Structure-Activity Relationship
D004798:Enzymes; D002245:Carbon Dioxide