Production of interleukin 1 and tumour necrosis factor activities in bronchoalveolar washings following infection of mice by influenza virus.
Mice were infected with influenza A virus by aerosol. Bronchoalveolar washings obtained from infected mice contained interleukin 1 (IL-1) and tumour necrosis factor (TNF) activities. IL-1 was present at day 4 post-infection but not at day 7. TNF activity was present at day 4 and day 7 post-infection. The presence of both these monokines was coincident with increased cell populations in the lungs. In vitro studies demonstrated that macrophages from non-infected mice produce IL-1 and TNF activities in response to live influenza A virus stimulation. These results suggest that a direct interaction between virus and alveolar macrophages leads to IL-1 and TNF production during the course of infection and could account for both the immune responses and the pathology that occur during influenza A virus infection.
Vacheron
F
F
Laboratoire de Microbiologie, UFR de Médecine Paris-Ouest, Hôpital Raymond-Poincare, Garches, France.
Rudent
A
A
Perin
S
S
Labarre
C
C
Quero
A M
AM
Guenounou
M
M
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology*; D005260:Female; D009980:Influenza A virus / Q000276:immunology*; D007375:Interleukin-1 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology; D051379:Mice; D009976:Orthomyxoviridae Infections / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms; D013601:T-Lymphocytes / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha