Primary structure and functional expression from complementary DNA of a human interleukin-1 receptor antagonist.
Human monocytes induced with adherent IgG secrete an interleukin-1 receptor antagonist which could be important for the in vivo regulation of IL-1 activity. A complementary DNA for this molecule has been isolated from a human monocyte library. Analysis of monocyte RNA indicates that the gene is transcriptionally regulated. The sequence of the receptor antagonist indicates that it is structurally similar to IL-1 beta. Expression of the cDNA in Escherichia coli yields IL-1 receptor antagonist activity.
Eisenberg
S P
SP
Synergen Inc., Boulder, Colorado 80301.
Evans
R J
RJ
Arend
W P
WP
Verderber
E
E
Brewer
M T
MT
Hannum
C H
CH
Thompson
R C
RC
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015232:Dinoprostone / Q000096:biosynthesis; D004926:Escherichia coli / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism; D015870:Gene Expression; D006031:Glycosylation; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000235:genetics / Q000494:pharmacology; D008969:Molecular Sequence Data; D009000:Monocytes / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D011506:Proteins / Q000235:genetics* / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D011971:Receptors, Immunologic / Q000037:antagonists & inhibitors*; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology; D012689:Sequence Homology, Nucleic Acid; D012795:Sialoglycoproteins
C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D011506:Proteins; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins; D012795:Sialoglycoproteins; D004247:DNA; D015232:Dinoprostone