Application of fast cyclic voltammetry to measurement of electrically evoked dopamine overflow from brain slices in vitro.
Fast cyclic voltammetry at a carbon fibre microelectrode was used to monitor the time course of dopamine overflow in slices of rat corpus striatum incubated in a brain slice chamber. Dopamine release occurred in response to electrical stimulation. Electrochemical, physiological and pharmacological evidence indicates that release of endogenous dopamine can be measured reliably for up to 9 h and that fast cyclic voltammetry can be used in brain slices for quantitative studies of dopamine release in the CNS.
Bull
D R
DR
Department of Neuropharmacology, Glaxo Group Research Ltd., Ware, Herts, U.K.
Palij
P
P
Sheehan
M J
MJ
Millar
J
J
Stamford
J A
JA
Kruk
Z L
ZL
Humphrey
P P
PP
D000818:Animals; D002118:Calcium / Q000502:physiology; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D004298:Dopamine / Q000378:metabolism*; D004558:Electric Stimulation; D004563:Electrochemistry; D066298:In Vitro Techniques; D008297:Male; D014179:Neurotransmitter Uptake Inhibitors / Q000494:pharmacology; D010879:Piperazines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013696:Temperature
D014179:Neurotransmitter Uptake Inhibitors; D010879:Piperazines; C043425:vanoxerine; D002118:Calcium; D004298:Dopamine