Two alkaline phosphatase genes are expressed during early development in the mouse embryo.
Alkaline phosphatase (AP) activity is stage specific in mouse embryos and may be associated with compaction and separation of trophectoderm from inner cell mass in preimplantation development. We previously sequenced a cDNA and two mouse AP genes that could contribute to the AP activity in embryos. Oligonucleotide primers were constructed from the three sequences and used in the reverse transcription-polymerase chain reaction technique to establish that two of the three AP isozymes are transcribed during preimplantation development. The predominant transcript (E-AP) is from a gene highly homologous to the human tissue-specific APs, but different from the mouse intestinal AP. Tissue non-specific (TN) AP also is transcribed, but there is approximately 10 times less TN-AP than E-AP transcript. The TN-AP isozyme is the predominant transcript of 7 to 14 day embryos and primordial germ cells. A switch in predominance from E-AP to TN-AP must occur during early postimplantation development. This study establishes a framework for experiments to determine the functions of the two isozymes during preimplantation development.
Hahnel
A C
AC
Department of Medical Biochemistry, University of Calgary Health Sciences Centre, Alberta, Canada.
Rappolee
D A
DA
Millan
J L
JL
Manes
T
T
Ziomek
C A
CA
Theodosiou
N G
NG
Werb
Z
Z
Pedersen
R A
RA
Schultz
G A
GA
D000469:Alkaline Phosphatase / Q000032:analysis / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D001755:Blastocyst / Q000201:enzymology; D004622:Embryo, Mammalian / Q000201:enzymology; D047108:Embryonic Development; D005260:Female; D005854:Germ Cells / Q000201:enzymology*; D007422:Intestines / Q000196:embryology* / Q000201:enzymology; D007527:Isoenzymes / Q000032:analysis / Q000235:genetics*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D013737:Testis / Q000201:enzymology
D007527:Isoenzymes; D012333:RNA, Messenger; D000469:Alkaline Phosphatase