The N-terminus, C-terminus, and vessel dilator of the ANF prohormone are present in the urine and increase with ventricular fibrillation.
The N-terminus consisting of amino acids (a.a.) 1-98 (i.e., proANF 1-98), C-terminus (i.e., ANF; a.a. 99-126) and midportion of N-terminus consisting of a.a. 31-67 (proANF 31-67; Vessel Dilator) of the 126 a.a. ANF prohormone were present in the urine in 5-to-8-fold increased concentrations versus their plasma concentrations in 6 dogs under basal conditions. With acute coronary occlusion the right atrial plasma concentrations of these peptides increased two-to-three-fold, while in the urine only proANF 31-67 increased (3.5-fold). Ventricular fibrillation caused a 4-to-10-fold increased secretion into the right atrial chamber with a simultaneous 3-to-4.7-fold increase in the urine of proANF 1-98, proANF 31-67, and ANF. This investigation demonstrates that proANF 1-98, proANF 31-67 and ANF are normally present in urine and increase in the urine with cardiac stimuli that cause their release from the heart.
Wyeth
R P
RP
Department of Medicine, University of Arkansas for Medical Sciences, John L. McClellan Memorial Veterans Hospital, Little Rock 72205.
Ackerman
B H
BH
Vesely
D L
DL
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000097:blood / Q000652:urine*; D004285:Dogs; D006352:Heart Ventricles / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D009318:Natriuresis; D010446:Peptide Fragments; D011498:Protein Precursors / Q000097:blood / Q000652:urine*; D011863:Radioimmunoassay; D014693:Ventricular Fibrillation / Q000378:metabolism*
D010446:Peptide Fragments; D011498:Protein Precursors; C055659:atrial natriuretic factor prohormone (1-98); D009320:Atrial Natriuretic Factor