Selective cytotoxicity against human tumor cells by an anti-gastric cancer monoclonal antibody-mitomycin C conjugate.
An anti-gastric cancer monoclonal antibody, MGb2, was chosen to prepare MGb2-mitomycin C (MMC) conjugate. Four to five molecules of MMC were introduced into each molecule of antibody with the antibody activity well retained. The conjugate showed a highly selective cytotoxicity upon human gastric cancer cells KATO-III. In the 48-h exposure test, the cytotoxic effect of MGb2-MMC upon target cells was similar to that of free MMC, but much greater than that of normal mouse immunoglobulin-MMC conjugate. Instead, the MGb2-MMC showed a statistically less cytotoxic effect upon non-target cells. Imaging and biodistribution studies indicated that the MGb2 was still well localized in tumor tissue after its conjugation with MMC.
Li
S
S
Laboratory of Gastroenterology, Xijing Hospital, Fourth Military Medical University, Xi-an, China.
Zhang
X Y
XY
Chen
X T
XT
Zhang
S Y
SY
Chen
L J
LJ
Zhang
J L
JL
D000818:Animals; D000903:Antibiotics, Antineoplastic; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D006801:Humans; D000922:Immunotoxins / Q000494:pharmacology*; D051379:Mice; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology*; D009368:Neoplasm Transplantation; D013274:Stomach Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000903:Antibiotics, Antineoplastic; D000911:Antibodies, Monoclonal; D000922:Immunotoxins; D008937:Mitomycins; D016685:Mitomycin