title: Hypoxic neuronal injury in tissue culture is associated with delayed calcium accumulation.
abstract: Calcium accumulation and neuronal injury were studied after hypoxia in cerebrocortical cell cultures in vitro. Neuronal injury was associated with a delayed calcium accumulation, which was greatest 5-7 hours after hypoxic exposure. Antiexcitotoxic treatments with tetrodotoxin and magnesium chloride or the selective N-methyl-D-aspartate antagonist (+/-)-4-(3-phosphonopropyl)-2-piperazinecarboxylic acid prevented hypoxic calcium accumulation and neuronal injury even when added 3 hours after hypoxia, during reoxygenation. Rescue of the neurons after hypoxia by blocking the delayed calcium accumulation in this cell culture preparation suggests a "therapeutic window" determined by calcium entry.
authors: Parke-Davis Pharmaceutical Research Division, Warner-Lambert Company, Ann Arbor, Mich 48105.
authors: F W
authors: FW
authors: Marcoux
authors: A W
authors: AW
authors: Probert
authors: M L
authors: ML
authors: Weber
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D046508:Culture Techniques; D002534:Hypoxia, Brain / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D010879:Piperazines / Q000494:pharmacology; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors; D013779:Tetrodotoxin / Q000494:pharmacology
chemical_list: D010879:Piperazines; D016194:Receptors, N-Methyl-D-Aspartate; D013779:Tetrodotoxin; C050592:3-(2-carboxypiperazin-4-yl)propyl-1-phosphonic acid; D002118:Calcium