Enhanced capacity of females for early interferon-gamma production by natural killer-like cells following stimulation by staphylococcal enterotoxin A.
Staphylococcal enterotoxin A (SEA) induced the production of interferon-gamma (IFN-gamma) by spleen cells from ICR Swiss mice during the first 24 h of culture. Splenocytes from females produced higher levels of IFN-gamma than did those from males at 8, 12, and 16 h. By 20 h after SEA stimulation, IFN-gamma production by spleen cells from males was similar to that of females. The cell types involved in IFN-gamma production in this SEA/spleen cell system were analyzed by depletion studies. Removal of Thy-1+ cells by panning prevented production of IFN-gamma in the 24 h after SEA stimulation. In vivo depletion of asialo GM1+ (AGM1+) cells prevented production of IFN-gamma through 16 h of culture with SEA, but permitted a modest IFN-gamma response at 20 h that was similar in magnitude in both sexes. Following removal of L3T4+ and Lyt-2+ cells by panning, IFN-gamma production was detected at 12 h after SEA stimulation and maintained through 24 h of culture with cells from females producing higher levels of IFN-gamma. These data suggest that male ICR Swiss mice are deficient in the activity of Thy-1+, AGM1+, L3T4-, and Lyt-2- cells in the early (8-16 h) production of IFN-gamma following SEA stimulation of spleen cells.
Tokman
M G
MG
Center for Animal Research, Cornell University, Ithaca, NY 14853.
McFarland
H I
HI
Ball
E J
EJ
Bigley
N J
NJ
D000818:Animals; D002448:Cell Adhesion; D004768:Enterotoxins / Q000494:pharmacology*; D005260:Female; D000949:Histocompatibility Antigens Class II; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000096:biosynthesis*; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D012737:Sex Factors; D013154:Spleen / Q000166:cytology / Q000276:immunology
D004768:Enterotoxins; D000949:Histocompatibility Antigens Class II; C026066:enterotoxin A, Staphylococcal; D007371:Interferon-gamma