Integration of bacteriophage lambda into the cryptic lambdoid prophages of Escherichia coli.
Bacteriophage lambda missing its chromosomal attachment site will integrate into recA+ Escherichia coli K-12 and C at the sites of cryptic prophages. The specific regions in which these recombination events occur were identified in both lambda and the bacterial chromosomes. A NotI restriction site on the prophage allowed its physical mapping. This allowed us to identify the locations of Rac, Qin, and Qsr' cryptic prophages on the NotI map of E. coli K-12 and, by analogy, to identify the cryptic prophage in E. coli C as Qin. No new cryptic prophages were detected in E. coli K-12.
Lichens-Park
A
A
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Smith
C L
CL
Syvanen
M
M
D010582:Bacteriophage lambda / Q000235:genetics*; D015139:Blotting, Southern; D002876:Chromosomes, Bacterial; D004926:Escherichia coli / Q000235:genetics*; D005814:Genes, Viral; D005838:Genotype; D008242:Lysogeny; D010957:Plasmids; D011938:Rec A Recombinases / Q000235:genetics; D011995:Recombination, Genetic; D015183:Restriction Mapping
D011938:Rec A Recombinases