title: [High-level tetracycline-resistance of Neisseria gonorrhoeae].
abstract: High level tetracycline resistance of Neisseria gonorrhoeae is mediated by a 25.2-MD plasmid and is characterized by a minimal inhibitory concentration of 16 micrograms/ml or more. The 25.2-MD plasmid originated through the insertion of the streptococcal tetM determinant into the 24.5-MD transfer plasmid of N. gonorrhoeae. In the present study the prevalence of N. gonorrhoeae with high-level tetracycline resistance in the Heidelberg gonococcal population between 1981 and 1989 was determined. Among 1765 N. gonorrhoeae isolates, one strain with high-level tetracycline resistance was identified. The presence of the 25.2-MD plasmid in this strain was demonstrated by plasmid-agarose gel electrophoresis. The auxotype/serovar class proline-/IB-1 indicates the importation of this strain from the USA. The 25.2-MD recombinant plasmid, in contrast to the 24.5 MD transfer plasmid, has a rather wide host range, which is favourable to the occurrence both of high-level tetracycline resistance and of beta-lactamase plasmids in N. meningitidis.
authors: Universitäts-Hautklinik Heidelberg.
authors: P K
authors: PK
authors: Kohl
authors: G P
authors: GP
authors: Géraud
authors: H D
authors: HD
authors: Piotrowski
authors: D
authors: D
authors: Petzoldt
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D009344:Neisseria gonorrhoeae / Q000187:drug effects*; D010957:Plasmids / Q000235:genetics / Q000276:immunology*; D011815:R Factors; D012097:Repressor Proteins / Q000032:analysis*; D013753:Tetracycline Resistance / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D012097:Repressor Proteins; C034811:tetracycline resistance-encoding transposon repressor protein
vernacular_title: "High-level"-Tetrazyklinresistenz von Neisseria gonorrhoeae.