title: [Immunobiological activities of Veillonella parvula isolated from infected root canals].
abstract: The primary etiological agent of all periapical lesions has long been considered to be bacterial. Furthermore, bacteria from infected root canals are potential antigens capable of initiating immunological reactions in periapical tissues. The purpose of this study, therefore, was clarify the immunological potentials of Veillonella parvula (V. parvula), which was frequently isolated from root canals with periapical lesion. Immunobiological activities of V. parvula sonic extracts were investigated on the enhancement of monocytes migration, induction of interleukin-1 (IL-1) production, mitogenicity and polyclonal activation of B cell. Following results were obtained: 1. Both LPS and protein of V. parvula sonic extracts strongly enhanced the activity of human peripheral monocytes migration. 2. Induction of IL-1 on C3H/HeN mice macrophage by V. parvula sonic extracts were stronger than that of S. typhimurium LPS as positive control. 3. It was found that mitogenicity of LPS from V. parvula on splenocytes was stronger than that of the protein, however mitogenicity on thymocytes was not shown in both preparation. 4. The polyclonal activation of B cell on splenocytes of BALB/c nu/nu mice by V. parvula was induced by the protein and LPS. 5. These findings indicate that both the protein and LPS from V. parvula have a regulation of immunobiological responses against macrophages and lymphocytes.
authors: Department of Endodontics, Kanagawa Dental College.
authors: Y
authors: Y
authors: Nagashima
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D003788:Dental Pulp Diseases / Q000382:microbiology*; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens; D009000:Monocytes; D014678:Veillonella / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D007375:Interleukin-1; D008934:Mitogens