Anti-CD4 modifies the kinetics but not the magnitude of anti-CD3-induced interleukin 2 production in peripheral T cells.
We have studied the effect of T cell receptor (TcR) cross-linking and TcR-CD4 cross-linking on the induction of interleukin (IL) 2 and IL 2 receptor mRNA expression in peripheral T cells. We confirm our previous results using bioassays, suggesting that CD4 is involved in T cell activation by up-regulating the expression of IL 2 receptors. Furthermore, we show that this also concerns other functional T cell genes such as IL 4 and interferon-gamma. In contrast, similar levels of IL 1 mRNA are found in T cells activated with anti-CD3 with and without anti-CD4 antibody, suggesting that the induction of the IL 2 gene is regulated by TcR cross-linking alone and not influences by CD4. However, anti-CD4 appears to accelerate the induction of IL 2 mRNA in the T cell populations, presumably by a more rapid adhesion of the cells to the antibody-coated surface.
Jönsson
J I
JI
Max-Planck-Institut für Immunbiologie, Freiburg, FRG.
Eichmann
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000502:physiology*; D017252:CD3 Complex; D015704:CD4 Antigens / Q000502:physiology*; D003470:Culture Media; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000235:genetics; D007700:Kinetics; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000502:physiology*; D015375:Receptors, Interleukin-2 / Q000235:genetics; D013601:T-Lymphocytes / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D015704:CD4 Antigens; D003470:Culture Media; D007376:Interleukin-2; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell; D015375:Receptors, Interleukin-2