Expression of the wheat chloroplast gene for CF0 subunit IV of ATP synthase.
The nucleotide sequence of the wheat chloroplast atp I gene encoding CF0 subunit IV of ATP synthase has been determined. The gene encodes a polypeptide of 247 amino acid residues with high sequence similarity to subunit IV from other plant chloroplasts and from cyanobacteria. The polypeptide shows sequence homology to the C-terminus of the F0 alpha subunit of Escherichia coli ATP synthase and subunit 6 of mitochondrial ATP synthase. The atp I gene is co-transcribed with the atp H, atp F and atp A genes for other subunits of ATP synthase in wheat. A gene-fusion of most of the atp I coding region with cro'-lacI'-lacZ' has been constructed in pEX2 and the fusion-protein has been used to raise antibodies in rabbits. The antibodies react with a polypeptide of 17 kDa in wheat thylakoid membranes indicating that the wheat atp I gene is expressed at the protein level. A model for the organisation of the polypeptide in the thylakoid membrane with four membrane-spanning segments is proposed.
Höglund
A S
AS
Botany School, University of Cambridge, England, UK.
Plant
A L
AL
Gray
J C
JC
D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D002736:Chloroplasts / Q000201:enzymology*; D003001:Cloning, Molecular; D015870:Gene Expression; D017343:Genes, Plant; D008969:Molecular Sequence Data; D006180:Proton-Translocating ATPases / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology; D012689:Sequence Homology, Nucleic Acid; D014908:Triticum / Q000235:genetics*
D011993:Recombinant Fusion Proteins; D006180:Proton-Translocating ATPases