Stabilization of clathrin coats by the core of the clathrin-associated protein complex AP-2.
AP-2 is the class of clathrin-associated protein complex found in coated vesicles derived from the plasma membrane of eukaryotic cells. We demonstrate here, using a chemical method, that an AP-2 complex is an asymmetric structure consisting of one large alpha chain, one large beta chain, one medium AP50 chain, and one small AP17 chain. The complex has been shown to contain a core and two appendages. The AP core includes the small AP17 and the medium AP50 chains together with the amino-terminal domains of the large alpha and beta chains. One appendage corresponds to the carboxy-terminal domain of the beta chain. We find that as in the case of the beta chains, the carboxy-terminal portion of the alpha chains is an independently folded domain corresponding to the second appendage. We use limited tryptic proteolysis of clathrin/AP-2 coats to show the release of the appendages from the interior of the coats and the retention of the AP core by the remaining clathrin lattice. In addition, we find that the AP core stabilizes the coat and prevents its depolymerization. These results are consistent with the proposal that the AP core contains the binding site(s) for clathrin, while the alpha- and beta-chain appendages interact with membrane components of coated pits and coated vesicles.
Matsui
W
W
Department of Anatomy and Cellular Biology, Harvard Medical School, Boston, Massachusetts 02115.
Kirchhausen
T
T
D033962:Adaptor Protein Complex 2; D033983:Adaptor Protein Complex mu Subunits; D034002:Adaptor Protein Complex sigma Subunits; D033942:Adaptor Proteins, Vesicular Transport; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000648:ultrastructure; D002417:Cattle; D002966:Clathrin / Q000378:metabolism*; D003034:Coated Pits, Cell-Membrane / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism; D010750:Phosphoproteins / Q000737:chemistry* / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D012996:Solutions; D014357:Trypsin
D033962:Adaptor Protein Complex 2; D033983:Adaptor Protein Complex mu Subunits; D034002:Adaptor Protein Complex sigma Subunits; D033942:Adaptor Proteins, Vesicular Transport; D000596:Amino Acids; D002966:Clathrin; D046911:Macromolecular Substances; D010446:Peptide Fragments; D010750:Phosphoproteins; D012996:Solutions; C438885:adaptor protein complex 2, mu 1 subunit; D014357:Trypsin