[Intraportal injection of specific monoclonal antibody in nude mice with liver metastasis].
To assess the importance of monoclonal antibody route of administration, we compared the selective (intraportal) and systemic injection of specific radiolabeled monoclonal antibody, using a murine model of hepatic metastases from a human colorectal carcinoma. Tumor uptake was studied over time after injection of 0.1, 1.0 or 2.0 micrograms of a specific antibody (HT29-15) or an isotype-matched control (BL-3). Significantly higher tumor uptake and tumor/liver or tumor/blood uptake ratios were seen in animals receiving intraportal injection for all tested doses. Intraportal injection of specific monoclonal antibody resulted in significant improvement in metastases uptake; these findings could be applied to the diagnosis and treatment of hepatic metastases from colorectal cancer using radiolabeled monoclonal antibodies.
Rivoire
M
M
Département de Chirurgie, Centre Léon Bérard, Lyon.
Yoshida
K
K
Divgi
C
C
Niedzwiecki
D
D
Chapman
D
D
Sigurdson
E
E
D000230:Adenocarcinoma; D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage* / Q000032:analysis; D002985:Clinical Protocols; D003110:Colonic Neoplasms; D005260:Female; D007275:Injections, Intravenous; D008113:Liver Neoplasms / Q000378:metabolism / Q000556:secondary*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011169:Portal Vein; D012107:Research Design
D000911:Antibodies, Monoclonal
Injection intraportale d'anticorps monoclonal spécifique chez la souris nude porteuse de métastases hépatiques.