Hypoxic neuronal injury in tissue culture is associated with delayed calcium accumulation.
Calcium accumulation and neuronal injury were studied after hypoxia in cerebrocortical cell cultures in vitro. Neuronal injury was associated with a delayed calcium accumulation, which was greatest 5-7 hours after hypoxic exposure. Antiexcitotoxic treatments with tetrodotoxin and magnesium chloride or the selective N-methyl-D-aspartate antagonist (+/-)-4-(3-phosphonopropyl)-2-piperazinecarboxylic acid prevented hypoxic calcium accumulation and neuronal injury even when added 3 hours after hypoxia, during reoxygenation. Rescue of the neurons after hypoxia by blocking the delayed calcium accumulation in this cell culture preparation suggests a "therapeutic window" determined by calcium entry.
Marcoux
F W
FW
Parke-Davis Pharmaceutical Research Division, Warner-Lambert Company, Ann Arbor, Mich 48105.
Probert
A W
AW
Weber
M L
ML
D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D046508:Culture Techniques; D002534:Hypoxia, Brain / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D010879:Piperazines / Q000494:pharmacology; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors; D013779:Tetrodotoxin / Q000494:pharmacology
D010879:Piperazines; D016194:Receptors, N-Methyl-D-Aspartate; D013779:Tetrodotoxin; C050592:3-(2-carboxypiperazin-4-yl)propyl-1-phosphonic acid; D002118:Calcium