Effect of aflatoxin B1 on resistance induced by Bordetella bronchiseptica vaccine in rabbits.
The effects of aflatoxin B1 on the development of the immune response to oil-adjuvanted Bordetella bronchiseptica vaccine and on acquired resistance to bacterial challenge were studied in rabbits. The doses of aflatoxin used were insufficient to produce clinical intoxication. Rabbits were randomly assigned to three groups, each having six animals: control (T), vaccinated (V), and vaccinated plus aflatoxin (VA) at 0.05 mg/kg daily per os. Groups V and VA were vaccinated twice, and the three groups were subsequently challenged with virulent B. bronchiseptica. The average weight gain at weekly intervals was significantly reduced in group VA, and no statistically significant differences were found in the titers of agglutinating antibodies between groups V and VA. There were significant differences between groups V and VA in the extent and severity of the pneumonic process, group VA being most affected. Results indicated that agglutinating antibody titers were not related to the level of protection in the latter group. Other mechanisms, such as alveolar macrophage activity and cell-mediated immunity, were implicated in the impairment of the acquired resistance in rabbits subclinically intoxicated with aflatoxin.
Venturini
M C
MC
Institute of Pathology, Faculty of Veterinary Sciences, La Plata National University, Argentina.
Perfumo
C J
CJ
Risso
M A
MA
Gomez
C M
CM
Piscopo
M V
MV
Sala de Miguel
M
M
Godoy
H
H
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000494:pharmacology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001428:Bacterial Vaccines / Q000276:immunology*; D001884:Bordetella / Q000276:immunology*; D001885:Bordetella Infections / Q000276:immunology*; D007110:Immunity, Active / Q000187:drug effects; D008168:Lung / Q000473:pathology; D011817:Rabbits; D011897:Random Allocation; D015430:Weight Gain / Q000187:drug effects
D000348:Aflatoxins; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D016604:Aflatoxin B1