[Preparation and isolation of dansyladenylates as fluorescent substrates in reactions of the energy metabolism].
A method was developed for preparation of dansylated derivatives of adenine nucleotides characterized by fluorescence when being irradiated with UV-light. The involvement of dansylated ATP, ADP and AMP as substrate analogues in energy metabolism is demonstrated in the ATPase, hexokinase, pyruvate kinase and adenylate kinase reactions. The kinetics of the reactions is discussed.
Tkachenko
A G
AG
D000227:Adenine Nucleotides / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000031:analogs & derivatives / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism; D003619:Dansyl Compounds; D004734:Energy Metabolism; D005456:Fluorescent Dyes; D006593:Hexokinase / Q000378:metabolism; D007700:Kinetics; D011770:Pyruvate Kinase / Q000378:metabolism; D013379:Substrate Specificity
D000227:Adenine Nucleotides; D003619:Dansyl Compounds; D005456:Fluorescent Dyes; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D006593:Hexokinase; D011770:Pyruvate Kinase; D000251:Adenosine Triphosphatases
Preparativnoe vydelenie i ispol'zovanie dansiladenilatov v kachestve fluorestsiruiushchikh substratov v reaktsiiakh énergeticheskogo metabolizma.