Photosensitizing activity of water- and lipid-soluble phthalocyanines on Escherichia coli.
Escherichia coli, as most Gram-negative bacteria, is insensitive to the photosensitizing action of both lipid-soluble Zinc-phthalocyanine (Zn-Pc) and water-soluble Zinc-mono/disulfonated phthalocyanine (Zn-PcS). Photosensitivity can be induced by alteration of the outer membrane, as obtained by either induction of competence or treatment with Tris-EDTA. Both phthalocyanines largely bind at the level of the cytoplasmic membrane; however, Zn-PcS shows a superior photosensitizing activity as compared with Zn-Pc. Biochemical analyses performed on irradiated cells suggest that the cytoplasmic membrane is an important target of the photoprocess, while DNA is not involved.
Bertoloni
G
G
Institute of Microbiology, University of Padova, Italy.
Rossi
F
F
Valduga
G
G
Jori
G
G
van Lier
J
J
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D001426:Bacterial Proteins / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000032:analysis / Q000187:drug effects / Q000502:physiology*; D007211:Indoles / Q000494:pharmacology*; D054833:Isoindoles; D008027:Light; D008565:Membrane Proteins / Q000032:analysis; D009942:Organometallic Compounds / Q000494:pharmacology*; D012995:Solubility; D014169:Transformation, Bacterial; D015032:Zinc; D017967:Zinc Compounds
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D007211:Indoles; D054833:Isoindoles; D008565:Membrane Proteins; D009942:Organometallic Compounds; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine; C064328:zinc phthalocyanine sulfonate; C064329:zinc phthalocyanine disulfonate; D004492:Edetic Acid; D015032:Zinc