[Immunohistochemical localization of the chondroitin sulfate proteoglycan in demineralized rat periodontal tissue].
We investigated the immunohistochemical localization of the chondroitin sulfates (chondroitin-4 sulfate, -6 sulfate and dermatan sulfate) in demineralized rat periodontal tissue using monoclonal antibodies (2-B-6, 3-B-3). Also, fixative and demineralized methods were established using these monoclonal antibodies. The result showed that the most effective combination of fixative and demineralized methods was 2% glutaraldehyde 1% para-formaldehyde and 5% EDTA. Chondroitin-4 sulfate and dermatan sulfate were widespread in gingival connective tissue and periodontal membrane, with an especially strong response of dermatan sulfate shown along collagen fiber bundles. Chondroitin-6 sulfate was located in peripheral blood vessels. In alveolar bone, chondroitin-4 sulfate and dermatan sulfate were found inside Hareversion canals, Volkman's canals and lacunae. Chondroitin-6 sulfate was localized at peripheral blood in alveolar bone. In cementum, chondroitin-4 sulfate and dermatan sulfate were found at lacunae of cellular cementum and a part of embedding Sharpey's fiber.
Murahashi
Y
Y
Department of Periodontology, Asahi University School of Dentistry.
Shibutani
T
T
Nishino
W
W
Hori
T
T
Shiraki
M
M
Iwayama
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal; D011508:Chondroitin Sulfate Proteoglycans / Q000032:analysis*; D003871:Dermatan Sulfate / Q000032:analysis; D007150:Immunohistochemistry; D010510:Periodontal Diseases / Q000378:metabolism*; D051381:Rats; D017001:Tooth Demineralization / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D011508:Chondroitin Sulfate Proteoglycans; D003871:Dermatan Sulfate