Na(+)-dependent Mg2+ efflux from Mg(2+)-loaded rat thymocytes and HL 60 cells.
Mg(2+)-loaded rat thymocytes and HL 60 (human promyelocytic leukemia) cells expressed a high rate of Na(+)-dependent net Mg2+ efflux which was inhibited by two thirds by 1 mM amiloride. During net Mg2+ efflux, extracellular Na+ was taken up at a stoichiometric ratio of 2 Na+ to 1 Mg2+, indicating electroneutral Na+/Mg2+ antiport as found for erythrocytes. Na(+)-independent Mg2+ efflux was not significantly expressed in these cell types.
Günther
T
T
Institute of Molecular Biology and Biochemistry, Free University of Berlin, FRG.
Vormann
J
J
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002460:Cell Line; D006801:Humans; D007951:Leukemia, Myeloid / Q000378:metabolism* / Q000473:pathology; D008274:Magnesium / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000502:physiology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D012964:Sodium; D008274:Magnesium