title: Neurospora crassa A mating-type region.
abstract: The mating-type locus of the haploid filamentous fungus Neurospora crassa is a regulatory region that controls entry into the sexual cycle and prevents formation of mixed mating-type heterokaryons in the vegetative phase. The locus consists of alternative sequences called A and a. The A mating-type DNA sequence of Neurospora crassa is composed of a region of 5301 base pairs that has little similarity to the sequence present at the mating-type locus in an a mating-type strain. However, the sequences flanking the mating-type locus in the A haploid and a haploid genome are essentially identical. The region of the A mating-type sequence required for expression of the heterokaryon incompatibility and sexual functions has been localized to a single open reading frame (ORF) encoding a polypeptide of 288 amino acids. Sequence analysis of sterile, heterokaryon-compatible mutants reveals frameshift mutations in this same ORF. The putative 288-amino acid product has a region of similarity to the MAT alpha 1 polypeptide of Saccharomyces cerevisiae.
authors: Department of Physiological Chemistry, University of Wisconsin, Madison 53706.
authors: N L
authors: NL
authors: Glass
authors: J
authors: J
authors: Grotelueschen
authors: R L
authors: RL
authors: Metzenberg
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005784:Gene Amplification; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D006238:Haploidy; D000072235:Mating Factor; D008969:Molecular Sequence Data; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000235:genetics*; D010455:Peptides / Q000235:genetics*; D010675:Pheromones / Q000235:genetics; D012331:RNA, Fungal / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013104:Spheroplasts / Q000502:physiology; D014170:Transformation, Genetic
chemical_list: D010455:Peptides; D010675:Pheromones; D012331:RNA, Fungal; D000072235:Mating Factor