title: Magnesium-ions accelerate the formation of the phosphoenzyme of the (Ca2+ + Mg2+)-activated ATPase from plasma membranes by acting on the phosphorylation reaction.
abstract: Magnesium ions in the reaction medium at 37 degrees C increased up to 222 s-1 the kapp for phosphorylation by ATP of the Ca2(+)-ATPase of pig red cell membranes. This effect was observed after partial proteolysis with trypsin which makes the enzyme behave like the E1 conformer during phosphorylation. These findings lead to the conclusion that Mg2+ increased the rate of phosphorylation of the Ca2(+)-ATPase by acting directly on this reaction. The apparent dissociation constant of Mg2+ for this effect was 44 microM whereas the apparent dissociation constant for Mg2+ to accelerate the shift E2----E1 between conformers measured on the intact enzyme was 50 microM. This suggests that Mg2+ accelerated both reactions from a single class of site.
authors: Instituto de Química y Fisicoquímíca Biológicas (UBA-CONICET), Buenos Aires, Argentina.
authors: H P
authors: HP
authors: Adamo
authors: A F
authors: AF
authors: Rega
authors: P J
authors: PJ
authors: Garrahan
mesh_terms: D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000097:blood*; D000252:Calcium-Transporting ATPases / Q000097:blood*; D004910:Erythrocyte Membrane / Q000201:enzymology*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology*; D010710:Phosphates / Q000097:blood; D010766:Phosphorylation; D013552:Swine
chemical_list: D010710:Phosphates; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases; D008274:Magnesium