Immunoelectrophoresis of mycobacterial antigens.
Polyvalent antiserum to culture filtrate of H37 Ra M. tuberculosis was raised in rabbits. Monospecific antiserum was raised against M. tuberculosis antigen-5, prepared from the culture filtrates by immunoabsorbent affinity chromatography. On immunoelectrophoresis, antigen-5 demonstrated single precipitin arc against polyvalent and monospecific antisera. The culture filtrate antigen demonstrated multiple precipitin arcs against polyvalent antiserum and single precipitin are against monospecific antiserum. Antigen-5 could be isolated and characterized from the culture filtrate of H37 Ra M. tuberculosis. Immunoelectrophoresis could be one of the method to characterize the mycobacterial antigens prepared in the laboratory.
Radhakrishnan
V V
VV
Division of Pathology, Sree Chitra Tirunal Institute for Medical Sciences & Technology, Trivandrum, India.
Mathai
A
A
Rao
S B
SB
Sehgal
S
S
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D007122:Immunoelectrophoresis; D008297:Male; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D011817:Rabbits; D014373:Tuberculin / Q000032:analysis* / Q000302:isolation & purification
D000942:Antigens, Bacterial; C016603:Mycobacterium tuberculosis antigen 5; D014373:Tuberculin