[Purge of malignant cells from bone marrow by hematoporphyrin derivatives and light exposure in vitro].
During autologous bone marrow graft in treatment of malignant diseases, it is critical to purge malignant cells from the marrow. In the present study, the sensitivity to photodynamic inactivation of 3 leukemic cell lines was compared with their counterpart normal hematopoietic cells. After mouse leukemic L1210 cells were treated with a preparation of hematoporphyrin derivatives, YHpD, 10 micrograms/ml for 1 h and irradiated with black light (peak wavelength 395 nm, light intensity 0.6 mW/cm2) for 5 min, the survival rate of clonogenic cells decreased to less than 10%, while that of bone marrow granulocyte-macrophage progenitor cells (CFU-GM) in DBA/2 mice remained at nearly normal level (greater than 80%). Similar results were obtained when human leukemic HL-60 cells were compared to human CFU-GM and mouse leukemic L615 cells to CFU-GM in 615 strain mice. It is suggested that hematoporphyrin photoradiation may be useful for selectivity killing leukemic cells in bone marrow.
Xu
C X
CX
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Lin
L
L
Huang
Y Y
YY
Liu
H Y
HY
Wang
X R
XR
Wang
Y X
YX
Zhang
B L
BL
Li
X R
XR
Hou
Y H
YH
D000818:Animals; D001854:Bone Marrow Cells; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D017324:Hematoporphyrin Derivative; D006414:Hematoporphyrin Photoradiation; D006415:Hematoporphyrins / Q000494:pharmacology*; D006801:Humans; D007939:Leukemia L1210 / Q000473:pathology*; D007942:Leukemia, Experimental / Q000473:pathology*; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology*; D051379:Mice; D008811:Mice, Inbred DBA; D008815:Mice, Inbred Strains; D010778:Photochemotherapy; D011838:Radiation-Sensitizing Agents; D014407:Tumor Cells, Cultured / Q000187:drug effects
D006415:Hematoporphyrins; D011838:Radiation-Sensitizing Agents; D017324:Hematoporphyrin Derivative