Adult mastocytosis: an immunophenotypic and flow-cytometric investigation.
Skin mast cells of five patients with mastocytosis/urticaria pigmentosa and from four normal controls were immunophenotyped with a panel of 17 monoclonal antibodies; peripheral blood mononuclear cells from the patients were similarly labelled and analysed by flow cytometry. Cutaneous mast cells expressed leucocyte common antigen and markers of macrophage/monocyte lineage suggesting a common progenitor in the ontogeny of the two cell types. The mast cells from the patients differed from the controls, expressing HLA-DR in four cases, and this may be a feature of proliferating cells. The patients' peripheral blood T lymphocytes showed a marked increase in the activation markers HLA-DR and CD25 suggesting a contributory role, possibly via cytokines, in driving mast-cell proliferation.
Ormerod
A D
AD
Department of Dermatology, University of Aberdeen, U.K.
Herriot
R
R
Davidson
R J
RJ
Sewell
H F
HF
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D000943:Antigens, Differentiation / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D002455:Cell Division; D005260:Female; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000032:analysis; D006649:Histocompatibility Antigens / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D017493:Leukocyte Common Antigens; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D008407:Mast Cells / Q000502:physiology*; D008415:Mastocytosis / Q000276:immunology*; D008875:Middle Aged; D010641:Phenotype; D012867:Skin / Q000276:immunology*; D013601:T-Lymphocytes / Q000502:physiology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; D015415:Biomarkers; D006684:HLA-DR Antigens; D006649:Histocompatibility Antigens; D017493:Leukocyte Common Antigens