title: Regulation of CD23 expression, soluble CD23 release and immunoglobulin synthesis of peripheral blood lymphocytes by glucocorticoids.
abstract: Evidence was obtained that glucocorticoids are capable of modulating the CD23 expression and soluble(s) CD23 release of peripheral blood lymphocytes (PBL). We demonstrate that interleukin-2 (IL-2)- and IL-4-induced CD23 expression are susceptible to glucocorticoids to a different degree. Prednisolone suppressed the spontaneous and IL-2-induced CD23 expression on PBL of healthy donors. The IL-4-induced CD23 expression was influenced much less by prednisolone, but the expression kinetics was altered. The modulation of the expression kinetics appears to be due to a priming effect of prednisolone. Differences were also apparent when the susceptibility of PBL from healthy and atopic donors towards the effect of prednisolone on the IL-4-induced CD23 expression was studied. Preactivation of PBL with Staphylococcus aureus strain Cowan I abolished the differences. Prednisolone also suppressed the sCD23 release from unstimulated and IL-2- or IL-4-stimulated PBL and enhanced the immunoglobulin (E,G,A,M) synthesis of PBL. This enhancement appears to be due to a priming effect, since pre-stimulation of PBL with prednisolone was sufficient to enhance the immunoglobulin synthesis. The IL-4-induced IgE synthesis of PBL with or without spontaneous in vitro IgE synthesis was synergistically enhanced by glucocorticoids.
authors: Institut für Medizinische Mikrobiologie und Immunologie, Ruhr-Universität Bochum, FRG.
authors: A
authors: A
authors: Fischer
authors: W
authors: W
authors: König
mesh_terms: D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis* / Q000378:metabolism; D002478:Cells, Cultured; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000502:physiology; D015847:Interleukin-4 / Q000502:physiology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D011239:Prednisolone / Q000494:pharmacology*; D011961:Receptors, Fc / Q000032:analysis* / Q000378:metabolism; D017455:Receptors, IgE
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D007136:Immunoglobulins; D007376:Interleukin-2; D011961:Receptors, Fc; D017455:Receptors, IgE; D015847:Interleukin-4; D011239:Prednisolone