title: Cytoplasmic microinjection of immunoglobulin Gs recognizing RNA helices inhibits human cell growth.
abstract: We report here that nucleolar and cytoplasmic RNA in mammalian cells is recognized specifically by both experimentally induced monoclonal IgG unique for left-handed Z-RNA and by autoimmune mouse monoclonal IgG specific for ribosomal RNA. Nucleolar Z-RNA synthesis, like nucleolar ribosomal RNA synthesis, is inhibited by actinomycin D treatment and dimethylsulfoxide-induced differentiation. Immune anti-Z-RNA IgGs microinjected into living nuclei bind nucleolar RNA, and these complexes appear to be removed from the nucleus within minutes. Cytoplasmically microinjected monoclonal or polyclonal anti-Z-RNA IgGs specifically bind cytoplasmic RNA and inhibit cell multiplication. Microinjection of antibodies directed against double-stranded RNAs. Elevated ionic conditions, which in energy-minimized models can cause the walls of the groove in Z-RNA (but not Z-DNA) to approach each other and close, also prevent antibody binding to specific synthetic or cellular Z-RNA determinants. Our antibodies binding unique Z-RNA structures probably recognize antigens determined by the exposed 2'-OH ribose sugar-phosphate groups.
authors: Molecular Biology Department, SRI International, Menlo Park, CA 94025.
authors: D A
authors: DA
authors: Zarling
authors: C J
authors: CJ
authors: Calhoun
authors: B G
authors: BG
authors: Feuerstein
authors: E P
authors: EP
authors: Sena
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000302:isolation & purification; D000936:Antigen-Antibody Complex; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D002466:Cell Nucleolus / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000008:administration & dosage / Q000302:isolation & purification; D008113:Liver Neoplasms; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008845:Microinjections; D008961:Models, Structural; D009690:Nucleic Acid Conformation; D012334:RNA, Neoplasm / Q000276:immunology* / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000648:ultrastructure
chemical_list: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D012334:RNA, Neoplasm