Strategy for constructing somatic hybrids isolating the two derivative chromosomes in X;autosome translocations. Application to a female patient t(X;5) with Hunter syndrome.
X; autosomal translocations are excellent tools for genetic analysis because of the easy selection of clones isolating the derivative bearing the HPRT gene in somatic cell hybrids. We have developed a strategy to select clones isolating the other derivative avoiding fastidious and time consuming technics, mainly based on immunofluorescent screening using MIC 2 and MIC 5 antigenic markers and we have succeeded in isolating in a rodent context the two X;5 translocated derivative chromosomes of a female patient with Hunter syndrome. The location of MIC 5 gene was specified between the IDS and G6PD DXS369 (RN1), DXS296 (VK21c), and DXS304 (U62), DXS52 and F8c (F814) are proximal and distal from the breakpoint disrupting the IDS gene respectively.
Couillin
P
P
INSERM U73, Génétique et Pathologie Foetale, Paris, France.
Le Guern
E
E
Ravise
N
N
Grisard
M C
MC
Oostra
B A
BA
Boue
A
A
D000911:Antibodies, Monoclonal; D015139:Blotting, Southern; D002459:Cell Fusion; D002460:Cell Line; D002999:Clone Cells; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D006822:Hybrid Cells / Q000648:ultrastructure*; D016532:Mucopolysaccharidosis II / Q000235:genetics*; D014178:Translocation, Genetic / Q000235:genetics*; D014960:X Chromosome
D000911:Antibodies, Monoclonal