title: Differentiation of human in vivo antigen-induced lymphoblastoid B cell precursors can be prevented by natural killer cells.
abstract: Tetanus toxoid (tet) immunization of normal humans leads to the transient appearance in the circulation of large lymphoblastoid (LB) B cells, which spontaneously secrete IgG-tet in short-term cultures. The present work examines the effect of endogenous natural killer (NK) cells, as defined as CD 16+ cells, on the LB B cell subset. NK cell depletion by monoclonal antibody (Leu11) and complement (C)-mediated lysis from LB B cell containing populations eliminated 76 +/- 7% of the cytotoxic activity against 51Cr-K-562 cells, and, in contrast, enhanced 2.7 +/- 0.3 times IgG-tet secretion. Cells untreated, treated with Leu11 alone, C alone, or with an irrelevant monoclonal antibody and C were used as controls. Furthermore, the increased IgG-tet production after NK depletion could be reversed by adding autologous CD 16(+)-enriched populations. Limiting dilution analysis revealed that the NK removal enhancement of IgG-tet production was due to an augmentation (3.18 +/- 0.63 times) of the precursor frequency of spontaneous antibody-secreting cells in NK-depleted cells with respect to control populations. Finally, the spontaneous IgG-tet-producing cells present in NK-depleted and control populations were the same since they produced similar quantities of antibody on a per cell basis and exhibited identical large size, as determined by 1 x G sedimentation technique. NK cells, therefore, exert a strong inhibitory effect on LB B cells by preventing the development of a considerable part of their precursors. This mechanism might be of importance in the control of LB B cells in vivo.
authors: Servicio de Inmunologia, Hospital Ramon y Cajal, Madrid, Spain.
authors: A
authors: A
authors: Garcia-Alonso
authors: J A
authors: JA
authors: Brieva
mesh_terms: D000943:Antigens, Differentiation / Q000494:pharmacology; D001402:B-Lymphocytes / Q000166:cytology*; D002454:Cell Differentiation; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D011961:Receptors, Fc / Q000494:pharmacology; D017452:Receptors, IgG
chemical_list: D000943:Antigens, Differentiation; D011961:Receptors, Fc; D017452:Receptors, IgG