title: Evidence for alternative RNA splicing and possible alternative promoters in the human muscle phosphofructokinase gene at the 5' untranslated region.
abstract: Three mRNA species for human muscle phophofructokinase containing heterogeneous 5' untranslated sequences were identified through cDNA cloning. Type A mRNA was essentially the same as that reported previously (Nakajima, H., et al. (1987) FEBS Lett. 223, 113). Type B mRNA was considered to be the major gene product, which contained an extra non-coding sequence within the 5' untranslated region of type A mRNA. Amplification of mRNA by polymerase chain reaction revealed that types A and B mRNAs shared a common precursor RNA, and were alternatively spliced. Type C mRNA, homologous to the cDNA sequence from a placenta library (Sharma, P. M. et al., (1989) Gene 77, 177), was considered to be under the control of an alternative promotor.
authors: Second Department of Internal Medicine, Osaka University Medical School, Japan.
authors: H
authors: H
authors: Nakajima
authors: T
authors: T
authors: Yamasaki
authors: T
authors: T
authors: Noguchi
authors: T
authors: T
authors: Tanaka
authors: N
authors: N
authors: Kono
authors: S
authors: S
authors: Tarui
mesh_terms: D001483:Base Sequence; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008969:Molecular Sequence Data; D009132:Muscles / Q000502:physiology; D010732:Phosphofructokinase-1 / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012326:RNA Splicing
chemical_list: D010732:Phosphofructokinase-1