Genes responsive to the alteration of polyamine biosynthesis in neurospora crassa.
Wild-type Neurospora crassa grown in minimal medium was exposed to -difluormethyl ornithine (DFMO), a specific inhibitor of ornithine-decarboxylase (ODC-ase) activity. Protein-synthesis rates impaired by DFMO were restored by the addition of spermidine. The pattern on SDS-acrylamide gels displayed three newly synthesized polypeptides, p27, p31 and p99 after DFMO action in the absence of exogenous polyamine. The ODC-ase mutant (spe-1) grown in spermidine-supplemented medium did not show an induced polypeptide pattern. The lack of ODC-ase activity promotes the expression of p27- and p31-coding genes in both strains but transcription of p31 gene is shut-off after spermidine addition. Both transcripts are also accumulated after exposure to low cycloheximide doses or nutrient starvation. Another cycloheximide-inducible gene coding for p70 is also expressed under DFMO-treatment.
Grotewold
E
E
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI), Buenos Aires, Argentina.
Aisemberg
G O
GO
Taccioli
G E
GE
Judewicz
N D
ND
D000518:Eflornithine / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D009955:Ornithine Decarboxylase / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism*; D013095:Spermidine / Q000494:pharmacology
D004791:Enzyme Inhibitors; D011073:Polyamines; D009955:Ornithine Decarboxylase; D013095:Spermidine; D000518:Eflornithine