[Effect of lipopolysaccharide on biological properties and induction of alveolar bone resorption in rats].
A study was made on the effects of lipopolysaccharides (LPS) from Escherichia coli and Actinobacillus actinomycetemcomitans on mitogenic response and the release of calcium from mouse calvaria. LPS exhibited significant mitogenic activity on mouse spleen cells, and increased the release of calcium from mouse calvaria in vitro. The effect of LPS from E. coli on alveolar bone resorption in Wistar male rats was also studied. LPS was infused by an Alzet miniosmotic pump that was implanted subcutaneously on the backs of the rats. A catheter connected to the pump was brought up to the maxillary right second molar using a nylon ligature. In this way the rats were continuously infused for 7 days with LPS. Alveolar bone loss was measured by an image-analyser (Nexus 6411). Rats stimulated by LPS exhibited significant alveolar bone loss, but bone loss was not observed in rats stimulated by saline. Polymyxin B effectively inhibited the LPS-stimulated bone resorption.
Maki
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Department of Periodontology, School of Dentistry, Aichi-Gakuin University, Nagoya, Japan.
D016976:Aggregatibacter actinomycetemcomitans; D016301:Alveolar Bone Loss / Q000139:chemically induced* / Q000188:drug therapy; D000818:Animals; D002118:Calcium / Q000378:metabolism; D004926:Escherichia coli; D008070:Lipopolysaccharides; D051379:Mice; D011112:Polymyxin B / Q000627:therapeutic use; D051381:Rats; D011919:Rats, Inbred Strains
D008070:Lipopolysaccharides; D011112:Polymyxin B; D002118:Calcium