A synthetic protein corresponding to the entire vpr gene product from the human immunodeficiency virus HIV-1 is recognized by antibodies from HIV-infected patients.
The 95 amino acid-protein encoded by the non-structural vpr gene of the human immunodeficiency virus type 1 (LAV-1BRU isolate) was chemically synthesized by solid phase methodology. The synthetic vpr protein was characterized by amino acid analysis, sequence analysis, RP-HPLC, and urea-SDS PAGE. Using a radioimmunoassay, antibodies to the synthetic protein were detected in sera of 25% of HIV 1-seropositive patients tested. Western blot analysis suggested that the antibodies preferentially recognize the dimeric form of vpr.
Gras-Masse
H
H
Biomolecular Chemistry Facility, CNRS-1309, Pasteur Institute, Lille, France.
Ameisen
J C
JC
Boutillon
C
C
Gesquière
J C
JC
Vian
S
S
Neyrinck
J L
JL
Drobecq
H
H
Capron
A
A
Tartar
A
A
D000595:Amino Acid Sequence; D016336:Gene Products, vpr / Q000138:chemical synthesis / Q000235:genetics / Q000276:immunology*; D015483:HIV Antibodies / Q000097:blood*; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D054325:vpr Gene Products, Human Immunodeficiency Virus
D016336:Gene Products, vpr; D015483:HIV Antibodies; D054325:vpr Gene Products, Human Immunodeficiency Virus