title: Detection of the schistosome circulating cathodic antigen by enzyme immunoassay using biotinylated monoclonal antibodies.
abstract: We have developed an enzyme immunoassay (ELISA) for the quantification of the schistosome circulating cathodic antigen (CCA), a glycoprotein associated with the syncitium lining the gut of the parasite. A mouse monoclonal antibody of IgG3 isotype was used as coating (antigen-capture) antibody, while a biotinylated mouse monoclonal IgM was used as second (antigen-detecting) antibody. Streptavidin-alkaline phosphatase was used as enzyme label. The lower detection limit of the assay was 1.0 ng of the trichloroacetic acid soluble fraction of adult worm antigen (AWA-TCA) per ml, which corresponds to approximately 0.2 ng CCA per ml. The ELISA showed a linear range from 1.0 to 62.5 ng AWA-TCA per ml. Serum and urine samples of 16 individuals infected with Schistosoma mansoni (egg counts ranging from 5 to 4820 eggs per gram of faeces) were tested in the assay. Antigen titres ranged from less than 4-8192. This assay represents a considerable advantage in diagnosis of Schistosoma infections as it allows the detection and quantification of CCA in serum and urine in even lightly infected individuals.
authors: Laboratory of Parasitology, Medical Faculty, University of Leiden, The Netherlands.
authors: N
authors: N
authors: de Jonge
authors: P G
authors: PG
authors: Kremsner
authors: F W
authors: FW
authors: Krijger
authors: G
authors: G
authors: Schommer
authors: Y E
authors: YE
authors: Fillié
authors: D
authors: D
authors: Kornelis
authors: R J
authors: RJ
authors: van Zeyl
authors: G J
authors: GJ
authors: van Dam
authors: H
authors: H
authors: Feldmeier
authors: A M
authors: AM
authors: Deelder
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000032:analysis*; D001710:Biotin; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006023:Glycoproteins / Q000032:analysis*; D015801:Helminth Proteins / Q000032:analysis*; D006801:Humans; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; C091532:CCA protein, Schistosoma mansoni; D006023:Glycoproteins; D015801:Helminth Proteins; D001710:Biotin