title: Restricted activation of general amino acid control under conditions of glutamine limitation in Neurospora crassa.
abstract: In Neurospora crassa limitation for single amino acids normally results in increased formation of enzymes required for amino acid synthesis via 'general amino acid control'. Glutamine limitation, however, led to comparatively low and delayed derepression of enzyme synthesis. Nitrate reductase activity increased steeply under these conditions confirming that de novo protein synthesis could occur. Derepression levels were unaffected by addition of glutamine-derived metabolites. Only small and delayed increases in mRNA levels occurred for the anabolic enzyme genes arg-12, his-3 and trp-1 under conditions of glutamine limitation in contrast to the immediate and far larger increase found on histidine limitation. The trans-acting regulatory gene of general amino acid control in Neurospora, cpc-1, responded with a significant increase in mRNA level to histidine and to glutamine limitation. The restricted response of the amino acid synthesis genes could imply a post-transcriptional block to the positive regulatory function of cpc-1 under condition of glutamine limitation. The results suggest that the expression of general amino acid control is restricted under conditions of inadequate nitrogen supply.
authors: Institut für Angewandte Genetik, Universität Hannover, Federal Republic of Germany.
authors: J
authors: J
authors: Kolanus
authors: J
authors: J
authors: Michalczyk
authors: H J
authors: HJ
authors: Flint
authors: I B
authors: IB
authors: Barthelmess
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics; D000596:Amino Acids / Q000096:biosynthesis*; D000878:Anthranilate Synthase / Q000235:genetics; D015152:Blotting, Northern; D015966:Gene Expression Regulation, Fungal; D005809:Genes, Regulator; D005973:Glutamine / Q000378:metabolism*; D007700:Kinetics; D009492:Neurospora crassa / Q000235:genetics / Q000378:metabolism*; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D009954:Ornithine Carbamoyltransferase / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D000596:Amino Acids; D005973:Glutamine; D000429:Alcohol Oxidoreductases; C021358:histidinol dehydrogenase; D009565:Nitrate Reductases; D050901:Nitrate Reductase; D009954:Ornithine Carbamoyltransferase; D000878:Anthranilate Synthase; D009584:Nitrogen