Use of Peldri II as a sublimation dehydrant in place of critical-point drying in fracture-label cytochemistry and in backscattered electron imaging fracture-label.
Peldri II, a fluorocarbon compound solid at room temperature, was used as a sublimation dehydrant in place of critical-point drying (CPD) in freeze-fracture cytochemistry. Its applicability to the fracture-label replica method was demonstrated using freeze-fractured rat pancreas labeled with Helix pomatia lectin-gold complex and phospholipase A2-gold complex for the detection of glycoconjugates and phospholipids, respectively. No difference in morphology and labeling pattern was detected in CPD-treated and Peldri II-treated tissue samples. The use of Peldri II was further extended to freeze-fractured rat duodenum labeled with wheat germ agglutinin-ovomucoid-gold complex. Again comparable results were obtained when duodenal samples were prepared according either to the conventional CPD method or to the Peldri II sublimation method. Freeze-fractured replicas of hamster ovaries labeled with Ricinus communis I-gold complex revealed that Peldri II could also be used as a sublimation dehydrant for preparing tissue samples for examination in the scanning electron microscope by the backscattered electron imaging mode.
Kan
F W
FW
Department of Anatomy, Faculty of Medicine, Université de Montréal, Quebec, Canada.
D000818:Animals; D006224:Cricetinae; D003890:Desiccation; D004386:Duodenum / Q000648:ultrastructure; D005260:Female; D005466:Fluorocarbons; D005614:Freeze Fracturing; D006048:Gold Colloid, Radioactive; D008297:Male; D008854:Microscopy, Electron / Q000379:methods*; D010053:Ovary / Q000648:ultrastructure; D010179:Pancreas / Q000648:ultrastructure; D010984:Platinum; D051381:Rats; D011919:Rats, Inbred Strains
D005466:Fluorocarbons; D006048:Gold Colloid, Radioactive; C062793:Peldri II; D010984:Platinum