Isolation and characterization of a distantly related member of the beta-gamma crystallin super gene family from Xenopus.
In an attempt to isolate gamma crystallin genes from Xenopus genomic library, we have obtained seventeen clones which strongly hybridized to the gamma 1 cDNA probe from Rana temporaria. Of these four clones are characterized. A restriction map was derived by using single and multiple digests with a variety of enzymes. Hybridization selection of lens mRNAs and in vitro translation produced a protein of 26,000 daltons. These data suggest that the above clones may contain at least part of the gamma or beta crystallin sequences.
Shastry
B S
BS
Eye Research Institute, Oakland University, Rochester, Michigan 48309.
Reddy
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VN
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003459:Crystallins / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D007908:Lens, Crystalline / Q000378:metabolism; D008970:Molecular Weight; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics; D011896:Rana temporaria; D015183:Restriction Mapping; D014982:Xenopus laevis
D003459:Crystallins; D012333:RNA, Messenger; D004247:DNA