Structure and biosynthesis of the xylose-containing carbohydrate moiety of rice alpha-amylase.
Suspension-cultured cells of rice secrete alpha-amylase into the culture medium. It has been shown that the mature form of the alpha-amylase contains xylose-bearing N-linked oligosaccharide: (formula; see text) We demonstrate that suspension-cultured cells of rice secrete alpha-amylase containing oligomannose-type oligosaccharides in the presence of 1-deoxymannojirimycin or tris(hydroxymethyl)aminomethane. On the other hand, alpha-amylase purified from germinated rice seedlings contains several kinds of oligomannose-type and N-acetyllactosamine-type oligosaccharides. The processing pathway of oligosaccharide moieties in rice cells is discussed on the basis of a comparison of these oligosaccharides structures.
Hayashi
M
M
Research Institute for Biochemical Regulation, School of Agriculture, Nagoya University, Japan.
Tsuru
A
A
Mitsui
T
T
Takahashi
N
N
Hanzawa
H
H
Arata
Y
Y
Akazawa
T
T
D017485:1-Deoxynojirimycin; D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D005944:Glucosamine / Q000031:analogs & derivatives / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis / Q000096:biosynthesis*; D012275:Oryza / Q000201:enzymology*; D013696:Temperature; D014415:Tunicamycin / Q000494:pharmacology; D014994:Xylose / Q000032:analysis*; D000516:alpha-Amylases / Q000032:analysis / Q000096:biosynthesis*
D009844:Oligosaccharides; D014415:Tunicamycin; D017485:1-Deoxynojirimycin; D014994:Xylose; D000516:alpha-Amylases; D005944:Glucosamine