[Elaboration of a semiautomated enzymatic analyzer of glucose for the control of biosynthesis of antibiotics].
The results of developing a semiautomatic apparatus with oxygen detection for enzymatic control of glucose concentration are presented. The design of a glucose sensitive electrode is based on an oxygen probe and a membrane with immobilized glucose oxidase. Materials for the probe were chosen and the operating conditions for measuring temperature, pH, linear agitation velocity and other parameters were optimized. The semiautomatic analyzer was constructed and its main characteristics were studied. The results of the apparatus testing during biosynthesis of various antibiotics are presented. It was shown that the required glucose concentration in the cultivation medium was provided for any specific circumstances in relation to the carbohydrate source.
Kraniauskas
V I
VI
Itsygin
S B
SB
Nesterov
V A
VA
Choban
P V
PV
Murygin
V G
VG
Bartoshevich
Iu E
IuE
Ralis
E V
EV
D000251:Adenosine Triphosphatases / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D003470:Culture Media; D004566:Electrodes; D004867:Equipment Design; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000494:pharmacology; D066298:In Vitro Techniques; D007218:Industrial Microbiology / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D008567:Membranes, Artificial; D011786:Quality Control; D014586:USSR
D000900:Anti-Bacterial Agents; D003470:Culture Media; D008567:Membranes, Artificial; D005949:Glucose Oxidase; D000251:Adenosine Triphosphatases; D005947:Glucose
Razrabotkapoluavtomaticheskogo fermentnogo analizatora gliukozy dlia kontrolia protsessov biosinteza antibiotikov.