Altered expression of proteoglycans in E1A-immortalized rat fetal intestinal epithelial cells in culture.
Normal and E1A-immortalized rat fetal intestinal epithelial SLC-11 cells were compared for the characteristics of the 35S-labeled proteoglycans isolated from their cell-associated and secreted fractions. In comparison with control cells in primary culture, immortalized SLC-11 cells: (a) secreted larger amounts of radiolabeled proteoglycans; (b) contained larger amounts of membrane-intercalated proteoglycans, analyzed by hydrophobic affinity chromatography on octyl-Sepharose; (c) produced cell-associated and secreted proteoglycans of smaller hydrodynamic size, assessed by measurement of Kav values; (d) contained a higher percentage of heparan sulfate in the cell-associated proteoglycans, determined by differential susceptibility of glycosaminoglycans to specific glycosaminoglycan lyases; (e) displayed heparan sulfate and chondroitin sulfate with a shorter chain length; and (f) synthesized glycosaminoglycans with a lower degree of sulfation, determined by ion-exchange chromatography. Taken together, these results demonstrate that in E1A-immortalized intestinal epithelial SLC-11 cells, the expression of proteoglycans alters considerably at an early stage of oncogenic transformation.
Lévy
P
P
Laboratoire de Biochimie, INSERM-U. 181, Faculté de Médecine Saint-Antoine, France.
Emami
S
S
Cherqui
G
G
Chastre
E
E
Gespach
C
C
Picard
J
J
D017456:Adenovirus Early Proteins; D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D002478:Cells, Cultured; D004848:Epithelium / Q000378:metabolism / Q000648:ultrastructure; D005333:Fetus; D006025:Glycosaminoglycans / Q000032:analysis; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000648:ultrastructure; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D011509:Proteoglycans / Q000032:analysis* / Q000737:chemistry; D011519:Proto-Oncogenes; D051381:Rats; D011919:Rats, Inbred Strains; D012995:Solubility
D017456:Adenovirus Early Proteins; D006025:Glycosaminoglycans; D009856:Oncogene Proteins, Viral; D011509:Proteoglycans