Interleukin-6 is the main mediator of the interaction between monocytes and platelets in the killing of Schistosoma mansoni.
We demonstrate the existence of a cooperation between monocytes and platelets for the killing of Schistosoma mansoni. Indeed, supernatants obtained after a 24 hr adherence of normal human monocytes were able to induce, in a dose dependent manner, the cytotoxicity of normal human platelets towards the young larvae of S.mansoni in vitro. The physicochemical analysis of the supernatants showed that a factor exhibiting a pl of 4.8-4.9 was responsible of this effect, suggesting a role of IL-6, detected in the supernatants, in this induction. This was confirmed by the neutralization of the cytotoxic effect by a polyclonal serum against IL6 whereas polyclonal sera against IL-1 beta or TNF-alpha, the other cytokines present in the supernatants, did not modify the cytotoxicity observed. Finally human recombinant IL-6 induces the platelet cytotoxic function, demonstrating a direct effect of IL6 on blood platelets.
Pancré
V
V
Unité mixte INSERM U 167 CNRS 624, Institut Pasteur, Lille, France.
Monté
D
D
Delanoye
A
A
Capron
A
A
Auriault
C
C
D000818:Animals; D001792:Blood Platelets / Q000276:immunology*; D002450:Cell Communication / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D006801:Humans; D066298:In Vitro Techniques; D015850:Interleukin-6 / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology
D015850:Interleukin-6