A contra-IL-2 cytokine derived from mice suppressed for collagen-induced arthritis.
The present study identifies and characterizes a cytokine derived from a T cell hybridoma which inhibits interleukin-2 (IL-2) function. The T cell hybridoma, T101N, was derived from somatic cell hybridization of lymphoid cells from mice suppressed for collagen-induced arthritis. Serial dilution of T101N cell culture medium reveals a concentration-dependent inhibition of recombinant IL-2 induced proliferation. Physiochemical properties of the inhibitor indicate that the contra-IL-2 activity is optimally resolved at 37 degrees C and neutral pH. Analysis of the molecular characteristics of the contra-IL-2 activity indicate that the cytokine activity is most biologically active as a pentimeric molecule of high molecular weight. Apparent molecular weight of monomeric contra-IL-2 is approximately 30,000-35,000 Da.
Kresina
T F
TF
Department of Medicine, Miriam Hospital, Providence, Rhode Island.
D000818:Animals; D001168:Arthritis / Q000276:immunology*; D001685:Biological Factors / Q000502:physiology*; D003094:Collagen; D016207:Cytokines; D006825:Hybridomas; D006863:Hydrogen-Ion Concentration; D007376:Interleukin-2 / Q000037:antagonists & inhibitors*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008970:Molecular Weight; D013601:T-Lymphocytes / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D013696:Temperature
D001685:Biological Factors; D016207:Cytokines; D007376:Interleukin-2; D003094:Collagen