Human prothymosin alpha: purification of a highly acidic nuclear protein by means of a phenol extraction.
Human prothymosin alpha, virtually alone among proteins, is recovered from the aqueous phase of phenol-extracted cell lysates prepared from human myeloma cells or COS cells that were transfected with the human prothymosin alpha gene. This observation forms the basis for purification of the protein to homogeneity in two steps--phenol extraction followed by electrophoresis in sodium dodecyl sulfate polyacrylamide gels to remove residual contaminants consisting chiefly of carbohydrate and RNA.
Sburlati
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Section on Genes and Gene Products, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
Manrow
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Berger
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D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D009101:Multiple Myeloma / Q000378:metabolism; D010636:Phenols; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D013947:Thymosin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D014162:Transfection
D010636:Phenols; D011498:Protein Precursors; D011994:Recombinant Proteins; C040782:prothymosin alpha; D013947:Thymosin