Lectin-binding glycoconjugates and immunoreactive proteoglycans in resorption pits freshly excavated by isolated rabbit osteoclasts.
Staining with FITC-conjugated concanavalin A, wheatgerm agglutinin and peanut agglutinin demonstrated that abundant sugar residues are present in resorption pits produced in vitro and over the cell surface of rabbit osteoclasts resorbing bovine femoral bone slices. After chondroitinase ABC digestion the stubs of chondroitin 4-sulphate and dermatan sulphate could be detected in the resorption pits by monoclonal antibodies.
Kanehisa
J
J
Department of Oral Pathology, Asahi University School of Dentistry, Gifu, Japan.
Shibutani
T
T
Iwayama
Y
Y
Takeuchi
H
H
D000818:Animals; D001862:Bone Resorption / Q000378:metabolism* / Q000473:pathology; D002417:Cattle; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000032:analysis; D003871:Dermatan Sulfate / Q000032:analysis; D005453:Fluorescence; D006001:Glycoconjugates / Q000032:analysis*; D006025:Glycosaminoglycans / Q000032:analysis; D037102:Lectins; D008855:Microscopy, Electron, Scanning; D010010:Osteoclasts / Q000378:metabolism* / Q000648:ultrastructure; D011509:Proteoglycans / Q000032:analysis*; D011817:Rabbits
D006001:Glycoconjugates; D006025:Glycosaminoglycans; D037102:Lectins; D011509:Proteoglycans; D003871:Dermatan Sulfate; D002809:Chondroitin Sulfates