A short domain of the plant vacuolar protein phytohemagglutinin targets invertase to the yeast vacuole.
Phytohemagglutinin (PHA), the seed lectin of the common bean, accumulates in protein storage vacuoles of storage parenchyma cells in cotyledons. When expressed in yeast, PHA is efficiently targeted to the yeast vacuole [Tague and Chrispeels (1987). J. Cell Biol. 105, 1971-1979]. To identify vacuolar sorting information in PHA, a series of 3' deletions of the PHA gene were fused in-frame to a truncated yeast invertase gene. An amino-terminal portion of PHA composed of a 20-residue signal sequence and 43 residues of the mature protein efficiently targeted invertase to the yeast vacuole. Internal deletions in a short PHA-invertase fusion showed that targeting information exists between residues 14 and 23 of mature PHA. Based on examinations of three-dimensional structures of related lectins, only a portion of these residues would be available on the surface of PHA for interaction with a putative receptor. Amino acid replacements at these positions in a PHA-invertase hybrid caused secretion of the invertase. The results indicate the presence of a vacuolar targeting domain in PHA that is centered at position 19 of the mature protein. This sequence of PHA also shows sequence identity to a vacuolar sorting domain characterized in yeast carboxypeptidase Y. Single amino acid alterations in a short PHA-invertase hybrid protein that caused the highest levels of secretion introduced a glycosylation site at position 21 of PHA. This observation suggests that glycan addition may interfere with recognition of a sorting determinant. These same amino acid changes did not dramatically increase secretion in a long PHA-invertase fusion or in PHA itself. Thus, a second domain of PHA may function in concert with the first one to bring about correct targeting of PHA.
Tague
B W
BW
Department of Biology, University of California, San Diego, La Jolla 92093-0116.
Dickinson
C D
CD
Chrispeels
M J
MJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D001692:Biological Transport; D007887:Fabaceae / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000032:analysis / Q000378:metabolism*; D008969:Molecular Sequence Data; D010835:Phytohemagglutinins / Q000235:genetics / Q000378:metabolism*; D037121:Plant Lectins; D010946:Plants, Medicinal; D021382:Protein Sorting Signals / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D017385:Sequence Homology; D014617:Vacuoles / Q000378:metabolism*; D043324:beta-Fructofuranosidase
D010835:Phytohemagglutinins; D037121:Plant Lectins; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase