Tissue specificity in expression and alternative RNA splicing of human phosphofructokinase-M and -L genes.
Mode of the expression of phosphofructokinase (PFK) -M and -L genes was examined in various human tissues including muscle, placenta, liver, kidney, pancreas, stomach and reticulocytes. The gross level of mRNA expression of PFK-M and -L genes was estimated by Northern analysis. Polymerase chain reaction was used to detect mRNA expressed at low levels in these tissues. Tissue-specific expression of alternatively spliced PFK-M gene transcripts was also determined by polymerase chain reaction. The results indicated that alternative splicing of PFK-M gene transcripts was controlled in a tissue-specific manner.
Nakajima
H
H
Second Department of Internal Medicine, Osaka University Medical School, Japan.
Kono
N
N
Yamasaki
T
T
Hamaguchi
T
T
Hotta
K
K
Kuwajima
M
M
Noguchi
T
T
Tanaka
T
T
Tarui
S
S
D001483:Base Sequence; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010732:Phosphofructokinase-1 / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D012333:RNA, Messenger / Q000378:metabolism*; D013379:Substrate Specificity
D012333:RNA, Messenger; D010732:Phosphofructokinase-1