[Laser nephelometry. A better methodology?].
Nephelometric assays (LN) are known since several years ago. Laser beam immunonephelometric analysis (He-Ne: 640 nm), are a sophistication of the spectrophotometers. Nephelometry was the first method to make use of narrow-angle light scattering and a laser beam. It was made 2500 +/- 200 determinations of human fluids for IgG, IgA, IgM, C3 and C4, both LN and RID (radial immunodiffusion method). Human patients had the following characteristics: 18-55 years old, both sexes and with different pulmonary pathologies (Bronchial asthma, Bronchitis, Tuberculosis, Emphysema, Fibrosis, Extrinsic allergic alveolitis). We found a greater sensitivity and a shorter analysis time for LN than for RID. On the other hand, Nephelometry gave different values to the radial immunodiffusion method for all the proteins evaluated.
Gaytán-Alcocér
M
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Instituto Nacional de Enfermedades Respiratorias, México, D.F.
D000293:Adolescent; D000328:Adult; D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex / Q000032:analysis; D003165:Complement System Proteins / Q000032:analysis*; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion; D007136:Immunoglobulins / Q000032:analysis*; D007834:Lasers; D008171:Lung Diseases / Q000097:blood* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation / Q000379:methods*
D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex; D007106:Immune Sera; D007136:Immunoglobulins; D003165:Complement System Proteins
Nefelometría de rayo láser. Una mejor metodología?