The effects of interleukin 2 on early and late thymocyte differentiation in foetal thymus organ culture.
The effects of interleukin 2 (IL-2) on in vitro T cell development in organ cultures of day 14 foetal mouse thymus were investigated. Over a 12 day culture period IL-2 was found to inhibit the development of each of the major thymocyte subpopulations, defined by CD4 and CD8 expression. Since each subpopulation was reduced in number, a common precursor to these subsets was thought to be inhibited by IL-2. Indeed, subsequent analyses of subsets of CD4- CD8- cells, characterized by expression of HSA, Pgp-1, and IL-2R, demonstrated that CD4- CD8- cells expressing IL-2R and their postulated progeny were reduced in IL-2-treated cultures. The number of cells reputed to be the immediate precursor of CD4- CD8- IL-2R+ cells, i.e. CD4- CD8- Pgp-1+IL-3R- cells, was not change by treatment with IL-2. Interestingly, however, numbers of the most immature subset of CD4- CD8- cells, identified as having the HSA1oPgp-1+IL-2R- and CD3- phenotype, increased in IL-2-treated cultures and was the only population of cells to do so. We also document the failure to detect any LAK cell activity against syngeneic thymocytes in IL-2-treated cultures and therefore hypothesize that the inhibition of T cell differentiation in these cultures is due to an arrest in differentiation of CD4- CD8- cells bearing the IL-2R.
Waanders
G A
GA
Department of Pathology and Immunology, Monash Medical School, Prahran, Victoria, Australia.
Boyd
R L
RL
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte; D015704:CD4 Antigens; D016827:CD8 Antigens; D002452:Cell Count; D002454:Cell Differentiation; D003602:Cytotoxicity, Immunologic; D005333:Fetus / Q000166:cytology / Q000276:immunology*; D007376:Interleukin-2 / Q000276:immunology / Q000494:pharmacology*; D051379:Mice; D009924:Organ Culture Techniques; D011948:Receptors, Antigen, T-Cell; D015375:Receptors, Interleukin-2; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology*; D013997:Time Factors
D000945:Antigens, Differentiation, T-Lymphocyte; D015704:CD4 Antigens; D016827:CD8 Antigens; D007376:Interleukin-2; D011948:Receptors, Antigen, T-Cell; D015375:Receptors, Interleukin-2