Effects of tumor necrosis factor alpha on parathyroid hormone-induced increases in osteoblastic cell cyclic AMP.
Tumor necrosis factor alpha (10(-10) - 10(-8) M) had no effects on cyclic AMP production by the osteoblastic osteosarcomal cells, Saos-2 and G292, or normal rat calvarial cells. The cytokine did, however, inhibit the parathyroid hormone (PTH)-induced effect on cyclic AMP in the Saos-2 and normal rat osteoblastic cells. This inhibitory effect did not occur on prostaglandin E2-induced cyclic AMP increases in the osteoblastic cells. Interleukin-1 (10 U/ml-100 U/ml) did not produce any effect on basal levels or PTH-induced cyclic AMP increases in these cells.
Shapiro
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S
SUNY-Buffalo, School of Dental Medicine, Department of Oral Biology 14214.
Tatakis
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DN
Dziak
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R
D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D015232:Dinoprostone / Q000378:metabolism; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism*; D012516:Osteosarcoma / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000494:pharmacology; D051381:Rats; D015398:Signal Transduction; D014407:Tumor Cells, Cultured / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D010281:Parathyroid Hormone; D014409:Tumor Necrosis Factor-alpha; D000242:Cyclic AMP; D015232:Dinoprostone