Structure of the glycosyl-phosphatidylinositol membrane anchor of the Leishmania major promastigote surface protease.
In common with many other plasma membrane glycoproteins of eukaryotic origin, the promastigote surface protease (PSP) of the protozoan parasite Leishmania contains a glycosyl-phosphatidylinositol (GPI) membrane anchor. The GPI anchor of Leishmania major PSP was purified following proteolysis of the PSP and analyzed by two-dimensional 1H-1H NMR, compositional and methylation linkage analyses, chemical and enzymatic modifications, and amino acid sequencing. From these results, the structure of the GPI-containing peptide was found to be Asp-Gly-Gly-Asn-ethanolamine-PO4-6Man alpha 1-6Man alpha 1-4GlcN alpha 1-6myo-inositol-1-PO4-(1-alkyl-2-acyl-glycerol). The glycan structure is identical to the conserved glycan core regions of the GPI anchor of Trypanosoma brucei variant surface glycoprotein and rat brain Thy-1 antigen, supporting the notion that this portion of GPIs are highly conserved. The phosphatidylinositol moiety of the PSP anchor is unusual, containing a fully saturated, unbranched 1-O-alkyl chain (mainly C24:0) and a mixture of fully saturated unbranched 2-O-acyl chains (C12:0, C14:0, C16:0, and C18:0). This lipid composition differs significantly from those of the GPIs of T. brucei variant surface glycoprotein and mammalian erythrocyte acetylcholinesterase but is similar to that of a family of glycosylated phosphoinositides found uniquely in Leishmania.
Schneider
P
P
Institut de Biochimie, Université de Lausanne, Epalinges, Switzerland.
Ferguson
M A
MA
McConville
M J
MJ
Mehlert
A
A
Homans
S W
SW
Bordier
C
C
D000595:Amino Acid Sequence; D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002462:Cell Membrane / Q000201:enzymology; D010450:Endopeptidases / Q000096:biosynthesis / Q000235:genetics*; D006017:Glycolipids / Q000096:biosynthesis* / Q000302:isolation & purification; D017261:Glycosylphosphatidylinositols; D007895:Leishmania tropica / Q000201:enzymology* / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism; D010716:Phosphatidylinositols / Q000096:biosynthesis* / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D014346:Trypanosoma brucei brucei / Q000235:genetics; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000235:genetics
D006017:Glycolipids; D017261:Glycosylphosphatidylinositols; D009227:Myristic Acids; D010716:Phosphatidylinositols; D014643:Variant Surface Glycoproteins, Trypanosoma; D019814:Myristic Acid; D010450:Endopeptidases