Identification within the simian virus 40 genome of a chromosomal loop attachment site that contains topoisomerase II cleavage sites.
We demonstrate that the simian virus 40 genome contains a single MAR (matrix association region) that maps within a large T-antigen coding region (nucleotides 4071 to 4377). This region contains topoisomerase II cleavage sites, exhibits sequence similarity with cellular MARs, and recognizes the same evolutionarily conserved, abundant nuclear binding sites seen by cellular MARs.
Pommier
Y
Y
Division of Cancer Treatment, National Cancer Institute, Bethesda, Maryland 20892.
Cockerill
P N
PN
Kohn
K W
KW
Garrard
W T
WT
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D001667:Binding, Competitive; D005075:Biological Evolution; D002467:Cell Nucleus / Q000378:metabolism; D002875:Chromosomes / Q000502:physiology; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism*; D005814:Genes, Viral; D006801:Humans; D007700:Kinetics; D015183:Restriction Mapping; D013539:Simian virus 40 / Q000235:genetics* / Q000276:immunology; D013379:Substrate Specificity
D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral; D004250:DNA Topoisomerases, Type II