Transforming growth factor-beta 1 stimulates expression of nerve growth factor in the rat CNS.
Transforming growth factor-beta 1 (TGF-beta 1) markedly increased the mRNA encoding nerve growth factor (NGF) in cultured rat astrocytes in a time- and concentration-dependent manner. The maximal effect of TGF-beta 1 (a 50-fold increase in NGF-mRNA) was reached after 24 h incubation. The TGF-beta-mediated increase in NGF-mRNA results from enhanced transcription as shown in nuclear run-on studies and in transfection assays using the NGF promoter linked to a chloramphenicol acetyltransferase (CAT) reporter gene. TGF-beta 1 also increased its own expression in astrocytes as well as that of the proto-oncogene c-fos. Intraventricular injection of TGF-beta 1 resulted in an increase of NGF-mRNA levels in the rat hippocampus (3-4 fold) showing that TGF-beta 1 is also effective in increasing NGF expression in vivo.
Lindholm
D
D
Department of Neurochemistry, Max-Planck-Institute for Psychiatry, Planegg-Martinsried, FRG.
Hengerer
B
B
Zafra
F
F
Thoenen
H
H
D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism; D015152:Blotting, Northern; D004247:DNA / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D008297:Male; D009414:Nerve Growth Factors / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D013552:Swine; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D009414:Nerve Growth Factors; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D004247:DNA