Death of serum-free mouse embryo cells caused by epidermal growth factor deprivation is prevented by cycloheximide, 12-O-tetradecanoylphorbol-13-acetate, or vanadate.
Serum-free mouse embryo cells cultured in medium supplemented with insulin, transferrin, high-density lipoprotein, and fibronectin are dependent on epidermal growth factor for survival. Cycloheximide or actinomycin D prevented cell death caused by growth factor deprivation, suggesting that cell death required the synthesis of RNA and protein, a phenomenon similar to that reported for neuronal cell death in the absence of nerve growth factor. Orthovanadate, an inhibitor of phosphotyrosine phosphatases, and 12-O-tetradecanoylphorbol-13-acetate, an activator of protein kinase C, also prevented serum-free mouse embryo cell death in the absence of epidermal growth factor.
Rawson
C
C
Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331-6503.
Cosola-Smith
C
C
Barnes
D
D
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology*; D004622:Embryo, Mammalian / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D004815:Epidermal Growth Factor / Q000187:drug effects / Q000494:pharmacology*; D066246:ErbB Receptors / Q000378:metabolism; D051379:Mice; D009414:Nerve Growth Factors / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000648:ultrastructure; D010703:Phorbol Esters / Q000494:pharmacology; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D010766:Phosphorylation; D011188:Potassium / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism; D017027:Protein Tyrosine Phosphatases; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014212:Tretinoin / Q000494:pharmacology; D014638:Vanadates / Q000494:pharmacology*
D009414:Nerve Growth Factors; D010703:Phorbol Esters; D014638:Vanadates; D014212:Tretinoin; D004815:Epidermal Growth Factor; D003513:Cycloheximide; D066246:ErbB Receptors; D011493:Protein Kinase C; D010749:Phosphoprotein Phosphatases; D017027:Protein Tyrosine Phosphatases; D000262:Adenylyl Cyclases; D013755:Tetradecanoylphorbol Acetate; D011188:Potassium