Cloning, sequencing and homologies of the cbh-1 (exoglucanase) gene of Humicola grisea var. thermoidea.
Studies on the enzymes of the cellulase complex of the thermophilic fungus Humicola grisea var. thermoidea are described. A genomic library was constructed in the phage vector EMBL 4, and from this library two clones were isolated using as a probe the cloned cbh-1 (exoglucanase, EC 3.2.1.91) gene of Phanerochaete chrysosporium, a cellulolytic basidiomycete fungus. These clones were analysed by restriction mapping and Southern blotting, and one of them (lambda 3) was sub-cloned into the M13 phage vectors mp18 and mp19. The gene sequence was determined by the dideoxy chain-termination method. Sequence comparison with the equivalent genes from P. chrysosporium and Trichoderma reesei was made: in terms of primary sequence there is about 60% homology between the three species. Secondary structure prediction of the H. grisea sequence was also computed.
Azevedo
M de O
Mde O
Department of Genetics, University of Leeds, UK.
Felipe
M S
MS
Astolfi-Filho
S
S
Radford
A
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003062:Codon; D004271:DNA, Fungal; D005091:Exons; D005800:Genes, Fungal; D043326:Glucan 1,3-beta-Glucosidase; D007438:Introns; D003904:Mitosporic Fungi / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D015183:Restriction Mapping; D016415:Sequence Alignment; D001617:beta-Glucosidase / Q000235:genetics* / Q000378:metabolism
D003062:Codon; D004271:DNA, Fungal; D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase