Inhibition of poly(ADP-ribose) synthetase by unsaturated fatty acids, vitamins and vitamin-like substances.
Various vitamins and vitamin-like substances inhibited the activity of poly(ADP-ribose) synthetase in vitro. The most potent were essential fatty acids, i.e. arachidonic acid, linoleic acid, and linolenic acid; their 50% inhibitory concentrations (IC50) were 44-110 microM, indicating a higher potency than nicotinamide, a well-known vitamin inhibitor (IC50 = 210 microM). Vitamins K3, K1, and retinal were the next strongest inhibitors, followed by alpha-lipoic acid, coenzyme Q0, and pyridoxal 5-phosphate. Nicotinamide and vitamin K3 exhibited mixed-type inhibition with respect to NAD+, while arachidonic acid exhibited dual inhibitions, competitive at 50 microM and mixed-type at 100 microM.
Banasik
M
M
Department of Clinical Science and Laboratory Medicine, Kyoto University Faculty of Medicine, Japan.
Komura
H
H
Ueda
K
K
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000494:pharmacology; D002417:Cattle; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D009536:Niacinamide / Q000494:pharmacology; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D013950:Thymus Gland / Q000201:enzymology; D014815:Vitamins / Q000494:pharmacology*
D001095:Arachidonic Acids; D005231:Fatty Acids, Unsaturated; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D014815:Vitamins; D009536:Niacinamide; D016718:Arachidonic Acid