A simple and rapid technique for identification of large numbers of individual mosquitoes using DNA hybridization.
A general method for obtaining species-specific repetitive DNA sequences is described. The method is based on the detection of recombinant DNA clones containing repetitive sequences using labeled total genomic DNA. These repetitive DNA sequences can be used to identify individual mosquito adults, pupae, and larvae squashed on filter membranes (squash blots). This technique was used to distinguish individuals of the four sibling species of the Anopheles quadrimaculatus complex. Repetitive DNA sequences and squash blots can be of use for rapid identification of other insect species in field collections.
Cockburn
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AF
USDA, ARS, Insects Affecting Man and Animals Research Laboratory, Gainesville, Florida 32604.
D000818:Animals; D000852:Anopheles / Q000235:genetics / Q000302:isolation & purification*; D004247:DNA / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
D004247:DNA