DOT-ELISA for detection of anti-Cysticercus cellulosae antibodies in human cerebrospinal fluid using a new solid-phase (resin-treated polyester fabrics). Preliminary report.
A dot enzyme-linked immunosorbent assay (DOT-ELISA) was developed to detect specific antibodies in cerebrospinal fluid (CSF) for human neurocysticercosis immunodiagnosis, with Cysticercus cellulosae antigen dotted on a new solid-phase. This was represented by sheets of a synthetic polyester fabric impregnated with a polymerized resin (N-methylol-acrylamide). A very stable preparation was thus obtained, the antigen being covalently bound by cross-linking with free N-methylol groups on the resin. Since robust, no special care was necessary for handling the solid-phase. The test could be performed at room-temperature. From 30 CSF samples assayed, 14 were positive, from a group of 15 cases of neurocysticercosis, with titers from 1 to 128; 15 other samples, from normals or other neurological diseases, were all negative. Test characteristics seem to indicate it as adequate for epidemiological surveys. A more detailed study on sensitivity, specificity, reproducibility and the use in serum samples is being conducted.
Vaz
A J
AJ
Instituto Adolfo Lutz-Divisão de Biologia Médica, São Paulo, Brasil.
Ferreira
A W
AW
Camargo
M E
ME
Nakamura
P M
PM
Camargo
E D
ED
D000818:Animals; D000909:Antibodies, Helminth / Q000134:cerebrospinal fluid*; D002493:Central Nervous System Diseases / Q000134:cerebrospinal fluid*; D003551:Cysticercosis / Q000134:cerebrospinal fluid*; D003552:Cysticercus / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D012117:Resins, Synthetic; D012680:Sensitivity and Specificity
D000909:Antibodies, Helminth; D012117:Resins, Synthetic