title: Plasmid and bacteriophage lambda-DNA show differential replication characteristics following injection into fertilized eggs of Xenopus laevis: dependence on period and site of injection.
abstract: The fate of DNA injected into in vitro fertilized eggs of Xenopus laevis during subsequent early embryogenesis was investigated by changing the time period and the area of injection. Form I/II plasmid DNA was found to be preferentially replicated in embryos which had been injected 60-65 min after fertilization into the animal half of the fertilized egg, irrespective of the presence of a eukaryotic origin of replication sequence element in the DNA probe used for injection. In the experiments where plasmid DNA and lambda-DNA were coinjected, only the latter was actively replicated, which suggests an inhibitory activity of this DNA on replication of coinjected plasmid DNA.
authors: Institute of Experimental Pathology, German Cancer Research Center, Heidelberg.
authors: A
authors: A
authors: Hofmann
authors: M
authors: M
authors: Montag
authors: H
authors: H
authors: Steinbeisser
authors: M F
authors: MF
authors: Trendelenburg
mesh_terms: D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D002970:Cleavage Stage, Ovum / Q000502:physiology; D004261:DNA Replication / Q000502:physiology*; D004279:DNA, Viral / Q000235:genetics; D008845:Microinjections; D010957:Plasmids / Q000235:genetics; D013997:Time Factors; D014982:Xenopus laevis / Q000196:embryology*; D015053:Zygote / Q000502:physiology*
chemical_list: D004279:DNA, Viral