title: The effect of splanchnic nerve stimulation on the uptake of atrial natriuretic peptide by the adrenal gland in conscious calves.
abstract: A technique has been developed with which it has been possible to quantify the output of a wide variety of agonists including catecholamines, glucocorticoids, mineralocorticoids, enkephalins and various peptides, from the adrenal gland in the conscious unrestrained calf; also to investigate responses to electrical stimulation of the peripheral end of the splanchnic nerve below any behavioural threshold. In the present study this methodology has been employed to investigate the extent to which stimulation of the splanchnic sympathetic innervation affects adrenal handling of atrial natriuretic peptide as this peptide has been identified within the adrenal medulla in this species. Stimulation of the splanchnic nerve at frequencies which raised the concentration of atrial natriuretic peptide like-immunoreactivity (ANP) by 25% led to an abrupt increase in the uptake of the peptide by the right adrenal gland by about 250%. During nerve stimulation more than 20% of the ANP that was estimated to be presented to the gland was taken up, by comparison with less than 13% of the amount presented which was taken up before and after stimulation. These results suggest that stimulation of the splanchnic nerve may specifically enhance the uptake of ANP by the adrenal gland and represent the first report of such a mechanism in respect of any biologically active peptide so far as we are aware.
authors: Physiological Laboratory, University of Cambridge, England.
authors: A V
authors: AV
authors: Edwards
authors: M A
authors: MA
authors: Ghatei
authors: S R
authors: SR
authors: Bloom
mesh_terms: D000311:Adrenal Glands / Q000098:blood supply / Q000378:metabolism*; D000818:Animals; D001158:Arteries; D009320:Atrial Natriuretic Factor / Q000097:blood / Q000378:metabolism*; D002417:Cattle; D004558:Electric Stimulation; D007700:Kinetics; D013153:Splanchnic Nerves / Q000502:physiology*; D014680:Veins
chemical_list: D009320:Atrial Natriuretic Factor