Cellular distribution, regulation, and biochemical nature of an Fc alpha receptor in humans.
In these studies, we characterize an Fc receptor (FcR) for IgA that is present on human granulocytes, monocyte/macrophages, and their corresponding cell lines. Receptor expression appears to be constitutive but can be selectively upregulated on monocyte cell lines by stimulation with a phorbol ester and polymeric IgA. Both the induction requirements and ligand specificity of the IgA receptor differ from the IgG receptors, Fc gamma R I, II, and III, that are also expressed on monocytes and granulocytes. IgA binding to the cell surface receptor is mediated via the Fc alpha region. The Fc alpha R is a heterogenously charged, approximately 60-kD molecule with an isoelectric point of 4.5-5.6 that binds monomeric or polymeric IgA1 and IgA2 molecules. This transmembrane glycoprotein appears to be composed of 32- and 36-kD protein cores with multiple N-linked carbohydrate moieties. We conclude that this Fc alpha R represents a novel member of the FcR family that may have a distinctive role in host defense.
Monteiro
R C
RC
Department of Medicine, University of Alabama, Birmingham 35294.
Kubagawa
H
H
Cooper
M D
MD
D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000032:analysis; D002460:Cell Line; D006098:Granulocytes / Q000032:analysis; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism* / Q000494:pharmacology; D009000:Monocytes / Q000032:analysis; D011961:Receptors, Fc / Q000032:analysis* / Q000378:metabolism; D017452:Receptors, IgG; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D015703:Antigens, CD; D000943:Antigens, Differentiation; C036832:Fc(alpha) receptor; D007070:Immunoglobulin A; D011961:Receptors, Fc; D017452:Receptors, IgG; C034639:polymeric IgA; D013755:Tetradecanoylphorbol Acetate