Treatment of HL-60 cells with dimethyl sulphoxide inhibits the formation of beta-N-acetylhexosaminidase S.
beta-N-Acetylhexosaminidase of HL-60 cells was separated into two main forms, A and S, by chromatography on DEAE-cellulose. Analysis of developmental changes in the isoenzyme pattern was complicated by the fact that the specific activity of beta-N-acetylhexosaminidase underwent a 6-fold change during the normal growth cycle. Two other lysosomal enzymes, beta-galactosidase and alpha-mannosidase, behaved similarly. Induction of differentiation of HL-60 cells with dimethyl sulphoxide at a low cell density (3 x 10(5) cells/ml) had a greater effect on the abundance of alpha-subunits of beta-N-acetylhexosaminidase, measured with 4-methylumbelliferyl-beta-N-acetylglucosaminide 6-sulphate, than of beta-subunits, measured with 4-methylumbelliferyl-beta-N-acetylglucosamine, and resulted in an isoenzyme profile in which A and B were the major forms, with the levels of form S greatly decreased.
Emiliani
C
C
Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Universita di Perugia, Italy.
Falzetti
F
F
Orlacchio
A
A
Stirling
J L
JL
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002848:Chromatography, DEAE-Cellulose; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D006026:Glycoside Hydrolases / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D015473:Leukemia, Promyelocytic, Acute; D008247:Lysosomes / Q000201:enzymology; D001619:beta-N-Acetylhexosaminidases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*
D006026:Glycoside Hydrolases; D001619:beta-N-Acetylhexosaminidases; D004121:Dimethyl Sulfoxide