[Quantitative determination of phenoloxidase (EC 1.14.18.1). Spectrophotometry measurement of the activity of mushroom and potato tyrosinase].
With dopa as substrat the enzyme were incubated 20 min in 0.1 M phosphate buffer, pH 6.8. After that the activity was detected at wavelengths of 313 and 492 nm using a layer thickness of 10 mm. Employing this conditions higher activities were estimated in the commercial preparates as indicated. The high sensitivity commends this standardized method to use in melanoma research.
Schmidt
H
H
Istitut für Biochemie des Bereichs Medizin, Martin-Luther-Universität, Halle-Wittenberg.
D001487:Basidiomycota / Q000201:enzymology; D014408:Biomarkers, Tumor / Q000032:analysis*; D006801:Humans; D008545:Melanoma / Q000175:diagnosis* / Q000201:enzymology; D014442:Monophenol Monooxygenase / Q000032:analysis*; D012878:Skin Neoplasms / Q000175:diagnosis* / Q000201:enzymology; D011198:Solanum tuberosum / Q000201:enzymology; D013053:Spectrophotometry
D014408:Biomarkers, Tumor; D014442:Monophenol Monooxygenase
Untersuchungen zur quantitativen Bestimmung von Phenoloxidase (EC 1.14.18.1), Spektrophotometrische Messungen der Aktivität von Pilz- und Kartoffeltyrosinase.