title: [In vitro processing of the artificial analog of beta-lipotropin].
abstract: The processing of the recombinant analogue of beta-lipotropin (beta-LHP) having 11 additional N-terminal amino acid residues and separated from the hormone by the processing signal, was investigated using rat adrenal secretory granule lysate as a test system of processing "in vitro". It was found that incubation of the beta-LPH analogue with secretory granule enzymes leads to its limited specific degradation with a release of native beta-endorphin. It is concluded that the additional N-terminal amino acids induced no qualitative changes in beta-LPH processing.
authors: A P
authors: AP
authors: Kuz'menko
authors: S A
authors: SA
authors: Fedosov
authors: S Ia
authors: SIa
authors: Golovin
authors: L V
authors: LV
authors: Mamaev
authors: V A
authors: VA
authors: Korzhov
authors: A A
authors: AA
authors: Il'ichev
authors: S N
authors: SN
authors: Zagrebel'nyĭ
mesh_terms: D000313:Adrenal Medulla / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D003594:Cytoplasmic Granules / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010957:Plasmids; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D001615:beta-Endorphin / Q000032:analysis; D008083:beta-Lipotropin / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D001615:beta-Endorphin; D008083:beta-Lipotropin
vernacular_title: Issledovanie protsessinga in vitro iskusstvennogo analoga beta-lipotropina.