Neurospora crassa cDNA clones coding for a new member of the ras protein family.
A new member of the ras gene family was characterized from Neurospora crassa cDNA libraries. The clone designated NC-ras codes for a polypeptide containing 213 amino acids (Mr 24,000). This polypeptide is 84% homologous to the H-ras-1 domain comprising the first 80 amino acids and 60% homologous to the next 84 residues. The NC-ras polypeptide contains all the well-known sequences involved in the interaction with GTP/GDP, the recognition of the Y13-259 neutralizing antibody, the 'effector site' for interaction with GAP proteins, and the CAAX acylation motif in the COOH-terminal.
Altschuler
D L
DL
Instituto de Investigaciones en Ingeniería Molecular (INGEBI-CONICET) Buenos Aires, Argentina.
Muro
A
A
Schijman
A
A
Almonacid
F B
FB
Torres
H N
HN
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes; D004271:DNA, Fungal / Q000235:genetics* / Q000302:isolation & purification; D005656:Fungal Proteins / Q000235:genetics*; D015723:Gene Library; D011905:Genes, ras; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D009492:Neurospora crassa / Q000235:genetics*; D012331:RNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D018631:ras Proteins
D015342:DNA Probes; D004271:DNA, Fungal; D005656:Fungal Proteins; D012331:RNA, Fungal; D018631:ras Proteins