Insulin-secreting beta-cells possess specific receptors for interleukin-1 beta.
The effect of the cytokine interleukin-1 beta on the insulin secretory responsiveness of single beta-cells (HIT-T15) was investigated. In the short-term, IL-1 beta induced a dosage-dependent stimulation of insulin release. In contrast, in the long-term, IL-1 beta, inhibited both basal and secretagogue-stimulated insulin secretion. We also demonstrate the simultaneous presence of specific high and low affinity binding sites for IL-1 beta on beta-cells. IL-1 beta, which has been implicated in the pathogenesis of insulin-dependent diabetes, may therefore mediate its opposing effects on beta-cells through a specific plasma membrane receptor.
Hammonds
P
P
Endocrinology Division, Glaxo Research Laboratories, Research Triangle Park, Durham, NC 27709.
Beggs
M
M
Beresford
G
G
Espinal
J
J
Clarke
J
J
Mertz
R J
RJ
D002118:Calcium / Q000502:physiology; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D004075:Diglycerides / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology; D005905:Glyburide / Q000494:pharmacology; D006706:Homeostasis; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007375:Interleukin-1 / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000502:physiology*; D017472:Receptors, Interleukin-1; D015290:Second Messenger Systems
D004075:Diglycerides; D007328:Insulin; D007375:Interleukin-1; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; D005576:Colforsin; D005947:Glucose; D005905:Glyburide; D002118:Calcium