Insulin therapy prevents spontaneous recovery from streptozotocin-induced diabetes in Syrian hamsters. An autoradiographic and immunohistochemical study.
Streptozotocin (Sz) given as a single dose of 50 mg/kg body wt. caused severe diabetes in Syrian hamsters. However, the level of blood glucose decreased gradually after 21 days post-Sz and reached the near normal level at 70 days in 90% of hamsters. The recovery from diabetes was associated with the regeneration of the beta-cells of islets and a reduction in the initially increased number of alpha- and delta-cells. Daily treatment of diabetic hamsters with insulin was associated with the persistence of severe diabetes, lack of or minimal tendency for beta-cell regeneration and sustained hyperplasia of alpha- and delta-cells in 90% of hamsters. Insulin also inhibited DNA synthesis (as measured by incorporation of tritiated thymidine), in ductal, ductular and acinar cells in Sz-pretreated hamsters but not in normoglycemic control hamsters treated with insulin alone. The results demonstrate a deleterious effect of exogenous insulin in the course of Sz-induced diabetes in hamsters.
Pour
P M
PM
Eppley Institute, University of Nebraska Medical Center, Omaha 68198.
Duckworth
W
W
Carlson
K
K
Kazakoff
K
K
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002455:Cell Division / Q000187:drug effects; D006224:Cricetinae; D003921:Diabetes Mellitus, Experimental / Q000473:pathology* / Q000503:physiopathology; D007328:Insulin / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D008297:Male; D010179:Pancreas / Q000166:cytology / Q000187:drug effects / Q000473:pathology*; D013311:Streptozocin
D001786:Blood Glucose; D007328:Insulin; D013311:Streptozocin