Annexin proteins PP4 and PP4-X. Comparative characterization of biological activities of placental and recombinant proteins.
The human placental proteins PP4 and PP4-X, belonging to the annexin protein family, were expressed in Escherichia coli at high yield. The proteins were purified to homogeneity. The physicochemical parameters of the recombinant proteins were determined and compared with those of their natural placental counterparts. Except for a minor change in the pI, the proteins appeared to be indistinguishable by several criteria. Both recombinant PP4 and recombinant PP4-X were biologically active in a thromboplastin inhibition test and in a phospholipase A2 inhibition test.
Römisch
J
J
Forschungslaboratorien der Behringwerke AG, Marburg/Lahn, Federal Republic of Germany.
Grote
M
M
Weithmann
K U
KU
Heimburger
N
N
Amann
E
E
D017304:Annexin A5; D000925:Anticoagulants / Q000302:isolation & purification*; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D015723:Gene Library; D006801:Humans; D005779:Immunodiffusion; D007700:Kinetics; D008970:Molecular Weight; D010314:Partial Thromboplastin Time; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology
D017304:Annexin A5; D000925:Anticoagulants; D001095:Arachidonic Acids; D002135:Calcium-Binding Proteins; D011257:Pregnancy Proteins; D011994:Recombinant Proteins; D016718:Arachidonic Acid; D010741:Phospholipases A; D054467:Phospholipases A2