Importance of DQB as an indicator in living-related kidney transplant.
Southern blot hybridization was performed in 16 pairs of living-related kidney transplant patients and donors, using DNA samples extracted from peripheral blood lymphocytes. The number of HLA-DNA-mismatched bands was used as an indicator for graft survival. The total number of DNA-mismatched bands seemed to be a valuable parameter. This was further analyzed and it was found that DQB in particular could be used to predict graft survival. Especially, usefulness of HLA-DNA typing was found in positive MLR, where good prognosis was strongly related to DQB matching. This method can therefore be applied in selecting a suitable living-related donor with the best chance of graft survival.
Matsuno
N
N
Department of Transplantation, Tokai University School of Medicine, Kanagawa, Japan.
Inoko
H
H
Ando
A
A
Nakatsuji
T
T
Sato
T
T
Itchikawa
S
S
Sonoda
T
T
Tsuji
K
K
D004247:DNA / Q000032:analysis; D005260:Female; D006085:Graft Survival; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics; D006801:Humans; D016030:Kidney Transplantation; D007959:Lymphocyte Culture Test, Mixed; D008297:Male
D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C057615:HLA-DQbeta antigen; D006684:HLA-DR Antigens; D004247:DNA