[Mouse interleukin-1 production by Actinobacillus actinomycetemcomitans Y4 lipopolysaccharide and augmenting effect of indomethacin on its production].
The present study demonstrates the ability of Actinobacillus actinomycetemcomitans lipopolysaccharide (LPS) to elicite interleukin-1 (IL-1) production and then augmenting effect of indomethacin on this production. LPS was isolated from A. actinomycetemcomitans Y4 by the hot phenol-water procedure (A-LPS). A marked IL-1 production by peritoneal macrophages treated with A-LPS was observed in the cells from C3H/HeN mice, but not in those from C3H/HeJ mice. LPSs from A. actinomycetemcomitans ATCC 29522 and 29523 exhibited the same inducing activity for IL-1 production as did A-LPS. The IL-1 production with A-LPS was inhibited drastically when the LPS was pretreated with polymyxin B. A-LPS-induced IL-1 production was augmented significantly by treatment of the cells with a inhibitor, indomethacin, but not with a lipooxygenase inhibitor, nordihydroguariatic acid. The augmenting effect occurred as early as 3 hr after treatment of indomethacin. In light of recent studies showing that IL-1 plays a regulatory role in bone remodeling systems, the present result suggest the possibility that A-LPS-induced IL-1 may play a significant role in mechanism(s) of alveolar bone resorption in juvenile periodontitis and also that its production may be regulated by prostaglandins.
Tsutsui
T
T
Department of Oral Microbiology, Meikai University School of Dentistry, Saitama, Japan.
Hanazawa
S
S
D016976:Aggregatibacter actinomycetemcomitans / Q000502:physiology*; D000818:Animals; D007213:Indomethacin / Q000494:pharmacology*; D007375:Interleukin-1 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000502:physiology*; D008264:Macrophages / Q000378:metabolism; D009637:Masoprocol / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D011112:Polymyxin B / Q000494:pharmacology
D007375:Interleukin-1; D008070:Lipopolysaccharides; D009637:Masoprocol; D011112:Polymyxin B; D007213:Indomethacin