title: Retinoic acid rapidly decreases phosphatidylinositol turnover during neuroblastoma cell differentiation.
abstract: Phosphatidylinositol (PI) turnover has recently been implicated in the regulation of cell proliferation and transformation. We have investigated its role in differentiation using LAN-1 cells, a human neuroblastoma cell line that can be induced to differentiate along the neuronal pathway by retinoic acid (RA). We have found that treatment of LAN-1 cells with RA is followed by a rapid decrease of inositol phospholipid metabolism, using myo-[1,2-3H]inositol or [1(3)-3H]glycerol. No changes were observed in both [3H]inositol and [3H]glycerol uptake within 24 h of RA treatment. Decreased incorporation of the metabolic precursor into PI 4-monophosphate and PI 4,5-bisphosphate occurred within 1 h of RA treatment. No changes were seen in the specific radioactivity of the precursor pools up to 1 h of treatment with RA. Analysis of labeled PI metabolites from prelabeled cells indicated a rapid decrease of inositol 1,4,5-trisphosphate and 1,2-diacylglycerol content within 1 min of induction of LAN-1 cell differentiation. These findings constitute the earliest reported events in neuroblastoma cell differentiation.
authors: Pediatric Oncology Research Laboratory, G. Gaslini Children's Hospital, Geona, Italy.
authors: M
authors: M
authors: Ponzoni
authors: M
authors: M
authors: Lanciotti
mesh_terms: D002454:Cell Differentiation; D003847:Deoxyglucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006801:Humans; D007294:Inositol / Q000378:metabolism; D007295:Inositol Phosphates / Q000378:metabolism; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology; D010716:Phosphatidylinositols / Q000378:metabolism*; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D007294:Inositol; D014212:Tretinoin; D003847:Deoxyglucose; D005990:Glycerol