Expression of the herpes simplex virus type 1 glycoprotein C gene requires sequences in the 5' noncoding region of the gene.
The role of the 5' noncoding region of the herpes simplex virus type 1 glycoprotein C (gC) gene in viral gene expression was investigated with recombinant herpesviruses that contained the bacterial beta-galactosidase gene under the control of the gC promoter-regulatory region. Each of these viruses had the same DNA sequences from the start of gC transcription upstream to -114 but had variable segments of the downstream 140-base-pair sequence that is between the start of gC transcription and translation. Analysis of beta-galactosidase expression and mRNA synthesis from these viruses demonstrated the importance of DNA sequences from the start of gC transcription downstream to +38 for optimal expression from the gC promoter.
Weir
J P
JP
Department of Microbiology, University of Tennessee, Knoxville 37996-0845.
Narayanan
P R
PR
D000818:Animals; D001483:Base Sequence; D002472:Cell Transformation, Viral; D015967:Gene Expression Regulation, Viral; D005798:Genes, Bacterial; D005814:Genes, Viral; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D011995:Recombination, Genetic; D018139:Simplexvirus / Q000235:genetics*; D014158:Transcription, Genetic; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics
D012333:RNA, Messenger; D014759:Viral Envelope Proteins; D015678:Viral Structural Proteins; C038535:glycoprotein gC, herpes simplex virus type 1; D001616:beta-Galactosidase