Differential increase in topoisomerase II in simian virus 40-infected cells.
The time course of expression of topoisomerase I, topoisomerase II, and simian virus 40 (SV40) large tumor (T) antigen was determined in whole-cell extracts of uninfected versus SV40-infected TC7 cells. After a minor increase, the level of topoisomerase I remained fairly constant throughout the time course in both uninfected and SV40-infected cells. In contrast, the level of topoisomerase II increased markedly in SV40-infected cells but not in uninfected cells following the appearance of SV40 T antigen.
Rainwater
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Biology Department, University of Alaska, Anchorage 99508.
Mann
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K
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D002460:Cell Line; D002472:Cell Transformation, Viral; D004261:DNA Replication; D004264:DNA Topoisomerases, Type I / Q000096:biosynthesis; D004250:DNA Topoisomerases, Type II / Q000096:biosynthesis*; D004279:DNA, Viral / Q000096:biosynthesis; D007700:Kinetics; D013539:Simian virus 40 / Q000201:enzymology / Q000235:genetics* / Q000276:immunology
D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral; D004264:DNA Topoisomerases, Type I; D004250:DNA Topoisomerases, Type II