Ultrastructural immunocytochemistry in glial tumors.
We have studied the ultrastructural distribution of GFAP, vimentin, FVIII/RAg and collagen IV in biopsies of human gliomas with various degree of anaplasia. Pre-embedding (vibratome and cryoultrathin sections) and post-embedding (LR White sections) techniques were tested. The immune reaction was demonstrated by peroxidase-DAB and gold labeling. In astrocytes, GFAP and VIM were localized in the same filament bundles; GFAP was always more expressed than vimentin, regardless of the malignancy grade. FVIII was localized in endoplasmic reticulum and in Weibel-Palade bodies. The influence of technical procedures on the optimal demonstration of each antigen is stressed.
Migheli
A
A
2nd Neurologic Clinic, University of Turin, Italy.
Mocellini
C
C
D003094:Collagen / Q000032:analysis*; D005169:Factor VIII / Q000032:analysis*; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis*; D005910:Glioma / Q000737:chemistry / Q000648:ultrastructure*; D006801:Humans; D007150:Immunohistochemistry; D014746:Vimentin / Q000032:analysis*
D005904:Glial Fibrillary Acidic Protein; D014746:Vimentin; D005169:Factor VIII; D003094:Collagen