title: Novel effect of aromatic compounds on the iron-dependent expression of the Escherichia coli K12 manganese superoxide dismutase (sodA) gene.
abstract: In Escherichia coli, the superoxide dismutase genes (sodA and sodB) sense the availability of Fe through the action of the fur locus [E. C. Niederhoffer, C. M. Naranjo, K. L. Bradley, J. A. Fee (1990) Control of Escherichia coli superoxide dismutases (sodA and sodB) genes by the ferric uptake regulation (fur) locus, J. Bacteriol. 172, 1930-1938]. Previous work from other laboratories has shown that a variety of metal chelators and of redox-active aromatic compounds can dramatically induce expression of sodA. Here we show that non-redox-active, non-metal-chelating aromatic compounds also enhance expression of a chromosomal sodA gene fusion and that these effects are strongly modulated by the Fur phenotype (Fur +/-) and by the availability of iron in the culture medium. The compounds studied were ethidium bromide, hemin, 2,2'-bipyridine, 1,10-phenantroline, 4,7-phenantroline, rhodamine B1, rhodamine 6G, and, for comparison to previous studies, Paraquat.
authors: Stable Isotope Resource, Los Alamos National Laboratory, NM 87545.
authors: E C
authors: EC
authors: Niederhoffer
authors: J A
authors: JA
authors: Fee
mesh_terms: D004996:Ethidium / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005798:Genes, Bacterial; D007501:Iron / Q000494:pharmacology*; D010269:Paraquat / Q000494:pharmacology; D004369:Pentetic Acid / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D011993:Recombinant Fusion Proteins; D004369:Pentetic Acid; D007501:Iron; D013482:Superoxide Dismutase; D001616:beta-Galactosidase; D004996:Ethidium; D010269:Paraquat