[Stability and gene expression of foreign plasmid in Bacillus subtilis BF7658].
The plasmid pAmy411 carrying thermostable alpha-amylase gene has been transduced into B. subtilis BF7658 by bacteriophage PBS1. The transduction frequency was 10(-9) transductants per PFU. The stability of plasmid pAmy411 in B. subtilis BF7658 was lower than that in B. subtilis AS1.1176, whereas the copy numbers were double than that in B. subtilis AS1.1176. The expressive level of thermostable alpha-amylase gene was about 6 times higher than that in B. subtilis AS1.1176.
Geng
Y Q
YQ
Department of Biology, Nankai University, Tianjin.
Jiang
R H
RH
Li
J
J
D001412:Bacillus subtilis / Q000235:genetics*; D015870:Gene Expression; D010957:Plasmids; D014161:Transduction, Genetic; D000516:alpha-Amylases / Q000235:genetics*
D000516:alpha-Amylases