Lipid peroxidation of rat liver microsomes membranes related to a protein deficiency and/or a PCB treatment.
In this study, we investigated the influence of protein deficiency on lipid peroxidation (LP) and cellular defense systems against oxidative damage in control or polychlorinated biphenyl (PCB) treated rats. Rats were fed either a standard diet (22% casein) or a low protein diet (3.5% casein) for 1, 2 and 6 weeks. Five days prior to killing, one half of the animals were given a single i.p. injection of Phenoclor DP6 (50 mg/kg body weight). In protein deficient rats, liver vitamin E was depressed and ascorbate level was lowered. Total and selenium-dependent glutathione peroxidases (GSH-Px) activities were decreased whereas glutathione reductase (GSH-red) was enhanced. Enzymatic and non enzymatic LP ('spontaneous' or with ADP-Fe2+) were increased. Phenoclor DP6 treatment enhanced liver ascorbate concentration. Microsomal LP was increased. Total and selenium-GSHPx remained unmodified while GSH-red was increased. Liver glutathione and alpha-tocopherol contents appeared to be independent of the PCB injection. Our data suggest that low protein intake and PCB exposure may reduce liver defensive protection against electrophilic species.
Pelissier
M A
MA
Laboratoire de Biologie du C.N.A.M., Réseau Français de Toxicologie Nutritionnelle, Paris, France.
Boisset
M
M
Atteba
S
S
Albrecht
R
R
D000818:Animals; D002364:Caseins / Q000008:administration & dosage*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000494:pharmacology*; D011488:Protein Deficiency / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D002364:Caseins; C013191:phenoclor; D011078:Polychlorinated Biphenyls; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase