HLA class II glycoprotein expression by monocytes from HIV-infected individuals: differences in response to interferon-gamma.
The expression of HLA class II glycoproteins by monocytes from HIV-infected individuals was evaluated. We found no differences in HLA-DR, HLA-DQ, or HLA-DP expression by freshly isolated monocytes from patients with clinical AIDS, HIV-infected patients without AIDS or from uninfected individuals. However, monocytes from HIV-infected individuals without AIDS responded better to stimulation with recombinant human interferon-gamma (IFN-gamma) than did monocytes from patients with clinical AIDS or from uninfected individuals. The response to IFN-gamma stimulation by monocytes from patients with clinical AIDS was different. Thus, the largest increases in the percentage of cells expressing HLA-DR was associated with the smallest changes in the fluorescence intensity of the population that is observed following stimulation of monocytes from other patient populations.
Zwilling
B S
BS
Department of Microbiology, Ohio State University, Columbus.
Darrow
R
R
Mcpeek
M
M
Laufman
H
H
Pearl
D
D
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology; D000704:Analysis of Variance; D002478:Cells, Cultured; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D015658:HIV Infections / Q000188:drug therapy / Q000276:immunology*; D006682:HLA-DP Antigens / Q000096:biosynthesis; D006683:HLA-DQ Antigens / Q000096:biosynthesis; D006684:HLA-DR Antigens / Q000096:biosynthesis; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D009000:Monocytes / Q000276:immunology*
D006682:HLA-DP Antigens; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D007371:Interferon-gamma