[Cloning and identification of erm gene and genes responsible for replication and stability of staphylococcal plasmid pE3692 determining the resistance to antibiotics of the MLS group].
Genetic elements coding for resistance to MLS group of antibiotics occur also in plasmids of staphylococcal cells. Wide distribution of such plasmids and their high segregational stability justify undertaking of detailed studies on their molecular structure and stability. It was shown that erm gene from pE3692 plasmid is located inside ClaI-HindIII segment of 1.3 kb dimension. Constructed pBE-9/1 plasmid, derivative of pE3692-9, contains inserted pBR322 vector in position ClaI. This plasmid replicates in E. coli and B. subtilis cells. It seems that Hind III-ClaI segment (0.8 hb) from pE3692 plasmid contains all indispensable genes for pE3692 replication. Excicion of Sta t (0.3 kb) fragment from pE3692 plasmid reduced considerably its stability. This segment may constitute a longer sequence fragment responsible for the stability of pE3692 plasmid.
Wieckiewicz
J
J
Zakład Mikrobiologii i Immunologii Instytutu Biologii Molekularnej UJ w Krakowie.
Wójcik
K
K
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003001:Cloning, Molecular; D003470:Culture Media; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial / Q000187:drug effects / Q000235:genetics*; D066298:In Vitro Techniques; D008034:Lincomycin / Q000494:pharmacology*; D018942:Macrolides; D011815:R Factors / Q000187:drug effects / Q000235:genetics*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics*; D014769:Virginiamycin / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D003470:Culture Media; D018942:Macrolides; D014769:Virginiamycin; D008034:Lincomycin
Klonowanie i identyfikacja genu ERM oraz genów odpowiedzialnych za replikacje i stabilność gronkowcowego plazmidu pE3692 determinujacego oporność na antybiotyki z grupy MLS.