Biological effects of adrenal androgens on MCF-7 and BT-20 human breast cancer cells.
The MCF-7 human breast cancer cell line responds to 5-androstene-3 beta,17 beta-diol, to dehydroepiandrosterone and to its 3 beta-sulfate stimulation in vitro by increased proliferation. 5-Androstene-3 beta,17 beta-diol has the strongest mitogenic effect. Dehydroepiandrosterone has a more potent action on cell proliferation than its 3 beta-sulfate. These adrenal androgens do not stimulate proliferation of the estrogen receptors negative for the BT-20 cell line. In our conditions, we have failed to demonstrate MCF-7 estradiol formation when incubations were carried out in the presence of labeled adrenal androgens. These results suggest that adrenal androgens stimulate proliferation of MCF-7 cells directly by acting on estrogen receptors.
Najid
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A
Laboratoire de Biochimie, Faculté de Pharmacie, Université de Limoges, France.
Habrioux
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G
D015114:Androstenediol / Q000494:pharmacology*; D000734:Androstenediols / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D003687:Dehydroepiandrosterone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D019314:Dehydroepiandrosterone Sulfate; D005260:Female; D006801:Humans; D011960:Receptors, Estrogen / Q000032:analysis / Q000187:drug effects; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000734:Androstenediols; D011960:Receptors, Estrogen; D003687:Dehydroepiandrosterone; D019314:Dehydroepiandrosterone Sulfate; D015114:Androstenediol