title: HLA-D determinants are expressed on human seminal cells other than spermatozoa but not on purified spermatozoa.
abstract: Purified human spermatozoa do not regularly stimulate lymphocytes in spermlymphocyte cultures (SLC). This lack of consistent stimulation was found not to be dependent on sperm/lymphocyte ratio or on culture peak response time. A few weak stimulations obtained are not HLA-D or sex dependent. Contaminating seminal cells other than spermatozoa (SC non-SZ) may be responsible for the high stimulations found in SLC by others, since, in our hands, purified suspensions of SC non-SZ stimulate allogeneic lymphocytes in an HLA-D-dependent fashion, and such responses are abrogated by anti-HLA-DR monoclonal antibodies. These functional data confirm our previous finding of HLA-DR molecules on SC non-SZ by absorptions (and a lack of expression on spermatozoa) and suggest the concomitant expression of HLA-DQ and -DP products.
authors: Department of Immunology, C.S.I.C., Madrid, Spain.
authors: S
authors: S
authors: Rodriguez-Cordoba
authors: J R
authors: JR
authors: Regueiro
authors: A
authors: A
authors: Arnaiz-Villena
mesh_terms: D000911:Antibodies, Monoclonal; D005260:Female; D006681:HLA-D Antigens / Q000235:genetics / Q000378:metabolism*; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D012661:Semen / Q000166:cytology / Q000276:immunology*; D013094:Spermatozoa / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D006681:HLA-D Antigens; D006684:HLA-DR Antigens