Characterization of a rice gene showing organ-specific expression in response to salt stress and drought.
Protein changes induced by salinity stress were investigated in the roots of the salt-sensitive rice cultivar Taichung native 1. We found eight proteins to be induced and obtained partial sequences of one with a molecular mass of 15 kilodaltons and an isoelectric point of 5.5. Using an oligonucleotide probe based on this information, a cDNA clone, salT, was selected and found to contain an open reading frame coding for a protein of 145 amino acid residues. salT mRNA accumulates very rapidly in sheaths and roots from mature plants and seedlings upon treatment with Murashige and Skoog salts (1%), air drying, abscisic acid (20 microM), polyethylene glycol (5%), sodium chloride (1%), and potassium chloride (1%). Generally, no induction was seen in the leaf lamina even when the stress should affect all parts of the plant uniformly. The organ-specific response of salT is correlatable with the pattern of Na+ accumulation during salt stress.
Claes
B
B
Laboratorium voor Genetica, Rijksuniversiteit Gent, Belgium.
Dekeyser
R
R
Villarroel
R
R
Van den Bulcke
M
M
Bauw
G
G
Van Montagu
M
M
Caplan
A
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000235:genetics; D012275:Oryza / Q000235:genetics*; D009997:Osmotic Pressure; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012492:Salts / Q000494:pharmacology
D015345:Oligonucleotide Probes; D010940:Plant Proteins; D012333:RNA, Messenger; D012492:Salts; C076973:SALT protein, Oryza sativa