Analysis of U1snRNP-specific A protein cross-linked complexes.
The organization of the U1snRNP-specific A protein (34 kDa) has been analyzed by 12 and 16 A thiol-reversible chemical cross-linking and Western blotting. A-containing cross-linked complexes had molecular masses of 43, 47, 56, 62, 67, 105 and 125 kDa. None of these complexes could be cross-linked following ribonuclease digestion, suggesting that UsnRNA may play important roles in the spatial organization of A and other proteins. Moreover, the data suggest that A is proximal to, and may have interactions with, UsnRNP-specific proteins C and 70 kDa as well as with UsnRNP-common proteins B, E and G.
Boix
J
J
Department of Pathology, University of Rochester, NY 14642.
Habets
W J
WJ
van Venrooij
W J
WJ
Smith
H C
HC
D015153:Blotting, Western; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006801:Humans; D007096:Imidoesters; D008970:Molecular Weight; D006163:Ribonuclease T1 / Q000378:metabolism; D012259:Ribonuclease, Pancreatic / Q000378:metabolism; D012261:Ribonucleoproteins / Q000737:chemistry* / Q000378:metabolism; D017411:Ribonucleoproteins, Small Nuclear; D013438:Sulfhydryl Compounds / Q000494:pharmacology
D003432:Cross-Linking Reagents; D007096:Imidoesters; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; D013438:Sulfhydryl Compounds; C007471:dimethyl dithiobispropionimidate; D006163:Ribonuclease T1; D012259:Ribonuclease, Pancreatic