Performance characteristics of mammalian cell culture process operating continuously with protein-free medium.
In this communication we briefly describe a continuous industrial process for the production of Tissue Plasminogen Activator (tPA), operating with a standard, basal medium (DME/F12). Process data of two culture runs has been chosen to present a performance profile over a production period of more than 3 mo. Developmental aspects of glucose utilization (as a process parameter) are briefly discussed.
Bliem
R
R
Bio-Response, Inc., Hayward, CA 94545.
Oakley
R
R
Matsuoka
K
K
Taiariol
V
V
D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D006801:Humans; D008545:Melanoma; D010100:Oxygen / Q000378:metabolism; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
D003470:Culture Media; D010959:Tissue Plasminogen Activator; D005947:Glucose; D010100:Oxygen