An efficient method for isolation of plasmid DNA from methylotrophic bacteria.
A method, suitable for the isolation of closed circular plasmid DNA from methylotrophic bacteria is described. Improvement of cell lysis was achieved by butanol extraction of cells before application of the lytic agent. Using this method, cryptic plasmids of 7.8, 14, 36 and 200 kb were purified from soil-isolated methylotrophs.
Brenner
V
V
Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
Holubová
I
I
Hubácek
J
J
D004269:DNA, Bacterial / Q000302:isolation & purification*; D004270:DNA, Circular / Q000302:isolation & purification*; D000432:Methanol / Q000378:metabolism; D008772:Methylococcaceae / Q000235:genetics* / Q000378:metabolism; D010084:Oxidation-Reduction; D010957:Plasmids; D012988:Soil Microbiology
D004269:DNA, Bacterial; D004270:DNA, Circular; D000432:Methanol