Estrogen effects on 5-HT1A receptors in hippocampal membranes from ovariectomized rats: functional and binding studies.
Chronic treatment (4 days) of ovariectomized rats with estrogen produced a two-fold shift to the left (with no change in the maximal percent inhibition) in the concentration response curve for the inhibition of adenylyl cyclase activity by 5-HT, but did not alter curves for R-PIA (adenosine A1 agonist) or Gpp(NH)p (to activate Gi). Furthermore, estrogen treatment had no effect on the number or affinity of 5-HT1A binding sites labeled with [3H]8-OH-DPAT. These data, when considered with the results of previous studies, suggest that estrogen treatment may selectively enhance 5-HT1A-mediated responses in rat hippocampus.
Clarke
W P
WP
Department of Pharmacology, Mount Sinai School of Medicine, CUNY, NY 10029.
Maayani
S
S
D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D005576:Colforsin / Q000494:pharmacology; D004343:Drug Implants; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D010052:Ovariectomy; D010660:Phenylisopropyladenosine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011985:Receptors, Serotonin / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D013764:Tetrahydronaphthalenes / Q000378:metabolism
D004343:Drug Implants; D011985:Receptors, Serotonin; D013764:Tetrahydronaphthalenes; D005576:Colforsin; D010660:Phenylisopropyladenosine; D006165:Guanylyl Imidodiphosphate; D004958:Estradiol; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin; D000262:Adenylyl Cyclases