title: In vitro growth of urinary Escherichia coli related to siderophore production.
abstract: Thirty seven strains of Escherichia coli isolated from the urine of patients with acute symptomatic urinary tract infection were examined for siderophore production: hydroxamate (aerobactin) and phenolate (enterochelin). All the strains were found to produce varying amounts of enterochelin. With the chemical assay, 24.3% strains were aerobactin producers, while 43.2% were positive in the bio-assay. All the aerobactin producers carried the aerobactin receptor on their surface. Attempts to correlate siderophore production with growth in minimal and iron-depleted medium showed that there was a positive quantitative correlation between enterochelin production and growth of organisms under iron depletion. Aerobactin production failed to give an additional advantage of growth to strains producing enterochelin.
authors: Department of Microbiology, Panjab University, Chandigarh, India.
authors: K
authors: K
authors: Harjai
authors: M
authors: M
authors: Saxena
authors: S
authors: S
authors: Chhibber
authors: S
authors: S
authors: Sharma
mesh_terms: D001425:Bacterial Outer Membrane Proteins; D004758:Enterobactin / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology; D006801:Humans; D006877:Hydroxamic Acids / Q000378:metabolism*; D007501:Iron / Q000378:metabolism / Q000494:pharmacology; D007502:Iron Chelating Agents / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000032:analysis; D012694:Serine / Q000031:analogs & derivatives*; D017262:Siderophores; D014552:Urinary Tract Infections / Q000382:microbiology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D006877:Hydroxamic Acids; D007502:Iron Chelating Agents; D011971:Receptors, Immunologic; D017262:Siderophores; C034506:aerobactin receptor; C031819:aerobactin; D004758:Enterobactin; D012694:Serine; D007501:Iron