Impaired conversion of exogenous arachidonic acid by platelets to thromboxane B2 and correction of that deficiency by interferon-alpha.
In the course of an investigation of cyclooxygenase and 12-lipoxygenase activity in platelets of patients with myeloproliferative syndrome receiving treatment with interferon-alpha 2 patients showed unusual results which have not been reported so far. Both patients had thrombocytosis, in one case associated with polycythaemia. In platelets of both patients, a reduced conversion of exogenous 14C arachidonic acid to TXB2 was observed accompanied by a shift in conversion to PGE2 and 12-HETE in one patient and to 12-HETE alone in the other before therapy. These findings were paradoxically associated with evidence of enhanced platelet activation in vivo. Treatment of both patients with interferon-alpha resulted in reversal of the biochemical abnormalities and in clinical remission.
Sinzinger
H
H
2nd Department of Internal Medicine, University of Vienna, Austria.
Linkesch
W
W
Ludwig
H
H
Gisslinger
H
H
O'Grady
J
J
Peskar
B A
BA
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000368:Aged; D001092:Arachidonate 12-Lipoxygenase / Q000097:blood; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000097:blood*; D001792:Blood Platelets / Q000378:metabolism*; D015232:Dinoprostone / Q000097:blood; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000097:blood; D007370:Interferon Type I / Q000627:therapeutic use*; D008297:Male; D009196:Myeloproliferative Disorders / Q000097:blood* / Q000628:therapy; D015539:Platelet Activation; D011087:Polycythemia Vera / Q000097:blood; D011451:Prostaglandin-Endoperoxide Synthases / Q000097:blood; D013920:Thrombocythemia, Essential / Q000097:blood; D013929:Thromboxane B2 / Q000097:blood*
D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D007370:Interferon Type I; D016718:Arachidonic Acid; D013929:Thromboxane B2; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D001092:Arachidonate 12-Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone