title: Competitive heterogeneous enzyme immunoassay for theophylline by flow-injection analysis with electrochemical detection of p-aminophenol.
abstract: A competitive enzyme-linked immunoabsorbent assay based on the flow-injection amperometric detection of p-aminophenol has been investigated with use of the materials and general procedure of a commercial kit for the determination of theophylline in human serum. The antibody is immobilized on glass beads, and the enzyme label is alkaline phosphatase (EC 3.1.3.1). The high currents generated during the electrochemical detection allowed a rapid (35 min) and simple determination of theophylline throughout its therapeutic range (10-20 mg/L) and also in the subtherapeutic range (detection limit of about 80 micrograms/L).
authors: Department of Chemistry, University of Cincinnati, OH 45221-0172.
authors: E P
authors: EP
authors: Gil
authors: H T
authors: HT
authors: Tang
authors: H B
authors: HB
authors: Halsall
authors: W R
authors: WR
authors: Heineman
authors: A S
authors: AS
authors: Misiego
mesh_terms: D000627:Aminophenols / Q000032:analysis*; D004563:Electrochemistry; D004566:Electrodes; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation*; D006801:Humans; D007124:Immunoenzyme Techniques; D011933:Reagent Kits, Diagnostic; D012212:Rheology; D013806:Theophylline / Q000097:blood* / Q000627:therapeutic use
chemical_list: D000627:Aminophenols; D011933:Reagent Kits, Diagnostic; D013806:Theophylline; C026729:4-aminophenol