Long-term preservation of donor hearts: the effect of intra- and extracellular-type of cardioplegic solutions on myocardial high energy phosphate content.
The influence of cardioplegic arrest (single or multidose cardioplegia) and subsequent long-term cold storage on myocardial high energy phosphate content was studied in 29 dogs divided into 6 groups of experiments. Three cardioplegic solutions were tested: Bretschneider HTK (intracellular-type solution), St. Thomas' Hospital and N.I.H. solutions (both extracellular-type solutions). In group I, II and III single dose cardioplegic arrest with respectively St. Thomas' Hospital, Bretschneider HTK and N.I.H. solutions was carried out and excised hearts were stored at 0.5 degrees C for 24 hours. In group IV-Bretschneider HTK and in group V-N.I.H. solutions were used for cardioplegic arrest and intermittent perfusion of the cooled hearts at 4, 8 and 12 hours of storage (multidose cardioplegia). In group VI, after cardioplegic arrest with Bretschneider HTK solution, different temperatures of storage (0.5 degrees C, 12 degrees C and 18 degrees C) were studied. Myocardial content of ATP and creatine phosphate was evaluated by means of bioluminescence techniques from serial left ventricular biopsies taken prior to aortic cross-clamping and after 1, 2, 3, 4, 5, 6, 8, 10, 12 and 24 hours. In group I ATP was significantly lower than in groups II and III after 6 h (p less than 0.005). After 24 hours of storage ATP-levels were significantly higher (p less than 0.05) after multidose Bretschneider HTK cardioplegia or N.I.H. cardioplegia than after single dose N.I.H. or St. Thomas' cardioplegia. There was no significant difference in ATP content between multidose Bretschneider and multidose N.I.H. cardioplegia.(ABSTRACT TRUNCATED AT 250 WORDS)
Dyszkiewicz
W
W
Department of Cardiovascular Surgery, Surgery Catholic University Leuven, Belgium.
Minten
J
J
Flameng
W
W
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002314:Cardioplegic Solutions; D015925:Cryopreservation; D004285:Dogs; D006321:Heart; D009206:Myocardium / Q000378:metabolism; D009926:Organ Preservation / Q000379:methods*
D002314:Cardioplegic Solutions; D000255:Adenosine Triphosphate