Ly1 and V-gene expression among hybridomas secreting natural autoantibody.
This report presents evidence that multispecific natural autoantibodies (NAAb) arise from both Ly-1+ and Ly-1- B lymphocyte subsets based upon detection of mRNA transcript of Ly1 gene by Northern blotting among hybridomas from several mouse strains. Newborn Xid mice also possess B cells transcribing the Ly-1 gene that secrete multispecific NAAbs. Different VH and VK gene families were expressed at random in both Ly-1+ and Ly-1- hybridoma clones secreting NAAbs. Certain VK gene families VK1, -8, -9, -10 and -19, were preferentially used by NAAbs. No particular VH:VK pairings were observed among NAAbs multispecifically reactive with DNA and cytoskeleton proteins. The NAAbs reactive with BrMRBC exhibited predominant pairing between VH11:VK9 gene families, although, for the first time, we noted the participation of VK1, -10 and -19 gene families in encoding this autoantibody specificity.
Kaushik
A
A
Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029.
Mayer
R
R
Fidanza
V
V
Zaghouani
H
H
Lim
A
A
Bona
C
C
Dighiero
G
G
D000818:Animals; D000950:Antigens, Ly / Q000235:genetics*; D001323:Autoantibodies / Q000235:genetics*; D001327:Autoimmune Diseases / Q000276:immunology*; D015152:Blotting, Northern; D015153:Blotting, Western; D002460:Cell Line; D015870:Gene Expression; D005803:Genes, Immunoglobulin; D006825:Hybridomas / Q000502:physiology*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains
D000950:Antigens, Ly; D001323:Autoantibodies; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains