The 49 K subunit of NADH: ubiquinone reductase (complex I) from Neurospora crassa mitochondria: primary structure of the gene and the protein.
The primary structure of the 49 K subunit of the respiratory chain NADH:ubiquinone reductase (complex I) from Neurospora crassa was determined by sequencing cDNA, genomic DNA and the N-terminus of the mature protein. The sequence lengths correlate to a molecular mass of 54,002 daltons for the preprotein and 49,239 daltons for the mature protein. The presequence consists of 42 amino acids of typical composition for sequences which target nuclear-encoded proteins into mitochondria. The mature protein consists of 436 amino acids and shows 64% similarity to a 49 K subunit of bovine heart NADH:ubiquinone reductase and 33% to a predicted translation product of an open reading frame in the chloroplast DNAs of Marchantia polymorpha and Nicotiana tabacum. Evidence for an iron-sulfur cluster in the subunit is discussed.
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Institut für Biochemie, Universität Düsseldorf, Federal Republic of Germany.
van der Pas
J C
JC
Nehls
U
U
Röhlen
D A
DA
Sackmann
U
U
Jahnke
U
U
Weiss
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D016660:NAD(P)H Dehydrogenase (Quinone); D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics*; D016366:Open Reading Frames; D011808:Quinone Reductases / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D016660:NAD(P)H Dehydrogenase (Quinone); D011808:Quinone Reductases