Screening of tetanus antibodies with the aid of dot-enzyme-linked immunosorbent assay (Dot-ELISA). Validation of the results using standard ELISA and passive hemagglutination test.
A Dot-ELISA is described that lends itself to the screening of large series of sera in relation to tetanus antibodies. A threshold of 0.06 I.U. per ml has been chosen as representing the protective tetanus antibody level. Two hundred and fifty-four sera were assayed by conventional ELISA, passive hemagglutination test and Dot-ELISA. The results obtained using concurrently these 3 techniques were in agreement with one another and permitted us to ascribe the same sera as tested by the various techniques to the same group of sera containing a non protective antitetanus antibody level and to the same group of sera-containing a protective level. It is suggested that Dot-ELISA should be used for the assessment of the tetanus immune status of populations.
Bizzini
B
B
Microbiology Institute, University of Messina, Italy.
Leonardi
M S
MS
Zummo
S
S
Mastroeni
P
P
D000907:Antibodies, Bacterial / Q000097:blood*; D003017:Clostridium tetani / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006386:Hemagglutination Tests; D006801:Humans; D007124:Immunoenzyme Techniques; D012680:Sensitivity and Specificity; D013745:Tetanus Toxoid / Q000276:immunology
D000907:Antibodies, Bacterial; D013745:Tetanus Toxoid