[Detection of antibodies to submitochondrial particles, F1-ATPase complex and liver specific lipoprotein in liver diseases using ELISA].
ELISA was elaborated for the determination of antibodies against liver antigen complexes--submitochondrial particles, F1-ATPase and liver specific lipoprotein. The parameters achieved so far allow to use the assay as an undemanding complementary laboratory technique in diagnosing and monitoring hepatopathies of autoimmune origin. Cross reactivity between individual antigen complexes was recorded in the majority of sera from positively reacting patients. The preliminary results show that individual antigen complexes have similar antigen structures bound to high-molecular membrane complexes, some of which, however, can be solubilized on maintaining antigen activity in ELISA.
Lochman
I
I
Oboru imunologie a alergologie KHS KUNZ v Ostravĕ.
Kulísková
L
L
Pribyl
B
B
Dostalík
Z
Z
Seflová
J
J
Machálek
J
J
Kozáková
D
D
Cebecauer
L
L
Havránková
J
J
D000818:Animals; D001323:Autoantibodies / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008107:Liver Diseases / Q000276:immunology*; D008297:Male; D008565:Membrane Proteins; D008930:Mitochondria, Liver / Q000276:immunology*; D011506:Proteins / Q000276:immunology*; D006180:Proton-Translocating ATPases / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D013367:Submitochondrial Particles / Q000276:immunology*
D001323:Autoantibodies; D008565:Membrane Proteins; D011506:Proteins; D006180:Proton-Translocating ATPases
Detekce protilátek proti submitochondriálnym cásticím, F1-ATP-ázovému komplexu a jaternímu specifickému lipoproteínu u jaterních onemocnĕní pomocí ELISA.