15-hydroxyeicosatetraenoic acid synthesis from exogenous arachidonate by bronchial mucosa.
Arachidonate metabolism in bronchial mucosa was investigated. Bronchial biopsies from four asthmatics and three non-asthmatic controls were challenged with 300, 465, 650 and 960 mOsm/kg saline in the presence of [3H]arachidonate. Supernatants were analysed by reverse-phase high-performance liquid chromatography. The major arachidonate metabolite was identified as 15-monohydroxyeicosatetraenoic acid (15-HETE), with significantly higher production in biopsies from asthmatics than controls. Statistical analysis of the full model explained 81% of the variation (F = 14.8; df = 6, 21; P less than 0.001). Hyperosmolarity and the presence of sarcoid had no significant effect on 15-HETE synthesis, reducing R2 by only 6%.
Ang
C
C
School of Medicine, St. George Hospital, University of New South Wales, Australia.
Clark
E
E
Young
I
I
Owe-Young
R
R
Eyland
A
A
Hunt
J
J
Chesterman
C
C
Krilis
S
S
D000328:Adult; D000368:Aged; D016718:Arachidonic Acid / Q000378:metabolism*; D001249:Asthma / Q000378:metabolism; D001980:Bronchi / Q000378:metabolism*; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008875:Middle Aged; D009092:Mucous Membrane / Q000378:metabolism
D006893:Hydroxyeicosatetraenoic Acids; D016718:Arachidonic Acid; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid