Intergeneric protoplast fusion between xylanase producing Bacillus subtilis LYT and Corynebacterium acetoacidophilum ATCC 21476.
Hybrids between a strain of Bacillus subtilis isolated in our laboratory and having the ability to degrade xylan and other complex polysaccharides and Corynebacterium acetoacidophilum, a lysine producer, were prepared by protoplast fusion. Based on distinctive parental biochemical characteristics the fusants were grouped into 9 categories, viz. BC1 through BC9. Three of the hybrids, BC5, BC7a and BC7b, were tested for their ability to produce xylanase and lysine. Both BC7a and BC7b produced xylanase but BC5 did not, however all of them produced lysine albeit to different degrees. These results demonstrate that intergeneric gene transfer takes place through protoplast fusion between these 2 important genera of bacteria and some of the fusants inherit the useful traits of both the parents.
Deb
J K
JK
Biochemical Engineering Research Centre, Indian Institute of Technology, New Delhi.
Malik
S
S
Ghosh
V K
VK
Mathai
S
S
Sethi
R
R
D001412:Bacillus subtilis / Q000201:enzymology*; D002459:Cell Fusion; D003352:Corynebacterium / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000235:genetics*; D006822:Hybrid Cells; D008239:Lysine / Q000235:genetics* / Q000378:metabolism; D011523:Protoplasts; D014162:Transfection; D043325:Xylan Endo-1,3-beta-Xylosidase
D006026:Glycoside Hydrolases; D043325:Xylan Endo-1,3-beta-Xylosidase; D008239:Lysine