Fetal pancreas transplantation in miniature swine. IV. Suppression of DTH and MLR responses by treatment with ultraviolet light-irradiated peripheral blood lymphocytes.
Irradiation of peripheral blood lymphocytes of miniature swine with ultraviolet light prevented them from initiating proliferative responses in allogeneic mixed lymphocyte reactions and also reduced IL-2 production in these MLRs. When pigs were injected in a series of 4-5 weekly transfusions with UV-irradiated allogeneic PBL differing at the MHC, PBL of recipient pigs progressively responded less strongly to donor PBL in MLRs over the treatment period. These pigs also gave negligible delayed-type hypersensitivity responses to donor PBL at the end of the treatment period. Of the seven UV-irradiated PBL-treated pigs, four produced no antidonor PBL antibody and three produced antibody. Serum from the three antibody-producing pigs also suppressed MLRs of unrelated PBL. By contrast, pigs that received a series of injections of untreated allogeneic PBL gave strong DTH responses to donor PBL and heightened proliferation in MLRs with donor PBL, and all produced antidonor PBL antibody.
Taura
Y
Y
Department of Medicine/Endocrinology, University of California, Los Angeles 90024.
Stein
E
E
Miyazawa
K
K
Mullen
Y
Y
D000818:Animals; D000906:Antibodies / Q000032:analysis; D001803:Blood Transfusion; D005260:Female; D005333:Fetus; D006968:Hypersensitivity, Delayed / Q000517:prevention & control*; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology / Q000528:radiation effects*; D008297:Male; D016035:Pancreas Transplantation; D013552:Swine; D013556:Swine, Miniature; D014466:Ultraviolet Rays
D000906:Antibodies