[A rapid diagnosis for periodontitis based on enzymatic activity of periodontopathic bacteria. Correlation between enzymatic activity, clinical periodontal parameters, and subgingival level of microorganisms].
There have been various laboratory methods for the microbiological diagnosis of periodontal disease. However, there have been some disadvantages in these methods. In this study of the application of a chair-side test for microbiological diagnosis, the activity of peptidases in periodontal pockets of patients was examined by using the assay system SK-013. SK-013 consists of synthetic substrates and is capable of rapidly (in 15 min) evaluating the activity of specific peptidase from Treponema denticola and Bacteroides species. Using SK-013, we evaluate the correlation between the enzymatic activity, clinical periodontal parameters and subgingival level of microorganisms, including phase contrast microscopy. We calculated the sensitivity and efficacy of SK-013 as a diagnostic indicator in the presence of Spirochetes and in periodontitis. A positive correlation were demonstrated between enzymatic activity, clinical periodontal parameters, the numbers of total cell count, Spirochetes, and M & S ratio. SK-013 was highly sensitive and efficacious (sensitivity: 92%, efficacy: 96%). We concluded that the assay system SK-013 is a useful chair-side method for diagnosing periodontal disease.
Ohtake
T
T
Department of Periodontics, Showa University.
Sakurai
C
C
Kurihara
C
C
Minamizaki
N
N
Kobayashi
M
M
Miyashita
H
H
Hasegawa
K
K
Kobayashi
T
T
Satoh
E
E
Sugita
N
N
D001439:Bacteroides / Q000201:enzymology; D001587:Benzoylarginine-2-Naphthylamide; D004796:Clinical Enzyme Tests / Q000379:methods*; D003773:Dental Plaque / Q000382:microbiology; D005188:False Negative Reactions; D005189:False Positive Reactions; D006801:Humans; D007202:Indicators and Reagents; D010447:Peptide Hydrolases / Q000032:analysis; D010518:Periodontitis / Q000175:diagnosis* / Q000382:microbiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013144:Spirochaetales / Q000201:enzymology; D014208:Treponema / Q000201:enzymology
D007202:Indicators and Reagents; D001587:Benzoylarginine-2-Naphthylamide; D010447:Peptide Hydrolases