Expression of human lysosomal beta-hexosaminidase in yeast vacuoles.
The yeast Saccharomyces cerevisiae was tested as a recipient for functional expression of a mammalian lysosomal enzyme. The beta chain of human beta-hexosaminidase formed active dimeric enzyme, HexB, in transformants. HexB activity was localized to the vacuole, the yeast counterpart to the lysosome. A simple in situ enzyme assay was developed, which could be useful in expressing other lysosomal cDNAs.
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Department of Biological Chemistry, UCLA School of Medicine 90024.
D054819:Hexosaminidase B; D006801:Humans; D008247:Lysosomes / Q000201:enzymology*; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D014617:Vacuoles / Q000201:enzymology*; D001619:beta-N-Acetylhexosaminidases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
D011994:Recombinant Proteins; D054819:Hexosaminidase B; D001619:beta-N-Acetylhexosaminidases