title: Genes expressed during conidiation in Neurospora crassa: molecular characterization of con-13.
abstract: Asexual development in Neurospora crassa proceeds through a series of discrete morphological stages that culminate in the production of dormant spores called conidia. Changes in the pattern of gene expression parallel the morphological transformations associated with conidiation. As a prerequisite to the analysis of developmental gene expression in N. crassa, several genes of unknown function that are preferentially expressed during conidiation were isolated [Berlin and Yanofsky, Mol. Cell. Biol. 5 (1985) 849-855]. The molecular structure and nucleotide sequence of one of these genes, designated con-13, is presented. The con-13 gene specifies a relatively rare 1.35-kb message which is first detected about 8 h following the induction of conidiation. Sequence analysis of both cDNA and genomic clones indicates that the con-13 gene consists of three exons divided by two small introns. It encodes a polypeptide of 340 amino acid residues (37.1 kDa). The Con-13 protein is weakly acidic and hydrophilic. A comparison of the regions upstream from the con-8, con-10, and con-13 genes revealed several short sequence motifs which may be important in developmental gene regulation.
authors: Department of Biological Sciences, Stanford University, CA 94305.
authors: K M
authors: KM
authors: Hager
authors: C
authors: C
authors: Yanofsky
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D016384:Consensus Sequence; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D005091:Exons; D005656:Fungal Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D007438:Introns; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000235:genetics* / Q000502:physiology; D010957:Plasmids; D015183:Restriction Mapping; D013172:Spores, Fungal; D014170:Transformation, Genetic
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; C066823:CON-13 protein, Neurospora crassa