title: Characterization of a series of monoclonal antibodies specific for bovine adenovirus serotype BAV3 hexon antigen and their use in an ELISA for detection of adenoviral hexons.
abstract: After immunization of mice with purified hexon (the main capsid antigen) of bovine adenovirus serotype BAV3 we have obtained a set of 16 individual hybridoma clones producing MAb's against BAV3 hexon. All MAb's were shown to belong to immunoglobulin G class. Specificity of the most avid MAb marked B3Hx-1 was tested on a panel of representative hexon antigens from 16 adenovirus serotypes of human and animal origin using several immunoassays. In Western blot analysis the MAb B3Hx-1 reacted only with native (trimeric) form of hexon protein and not with denaturated hexon polypeptide chains. The epitope defined by B3Hx-1 appeared stable against SDS at ambient temperature and against chloramine-promoted iodination. The specificity of the epitope was characterized as almost genus-crossreactive: it was absent from hexons of avian and of bovine subgroup 2 adenovirus serotypes and present in most hexons of bovine, canine, simian and human adenoviruses tested. Within the latter group its expression was weak or absent only for human subgenus C serotypes. Several variants of sandwich-type ELISA were developed using MAb B3Hx-1 and different polyclonal antibodies against hexons of mammalian adenoviruses. The level of hexon detection for different adenovirus serotypes varied in range 10(-9) to 10(-8) g per ml.
authors: Research Institute for Agricultural Biotechnology, Moscow, USSR.
authors: V R
authors: VR
authors: Isakova
authors: E K
authors: EK
authors: Kiseleva
authors: M A
authors: MA
authors: Kirasova
authors: M L
authors: ML
authors: Zelivyanskaya
authors: V G
authors: VG
authors: Grigoryev
authors: S N
authors: SN
authors: Khilko
authors: T I
authors: TI
authors: Tikchonenko
mesh_terms: D000256:Adenoviridae / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000914:Antibodies, Viral / Q000737:chemistry; D000915:Antibody Affinity; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology*; D001717:Birds; D002213:Capsid / Q000032:analysis* / Q000276:immunology; D036022:Capsid Proteins; D002417:Cattle; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D000882:Haplorhini; D006801:Humans; D006825:Hybridomas / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D013045:Species Specificity
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus