Expression of T-cell receptor (TCR)alpha chain on normal human tonsils and T-cell lymphomas.
We analyzed immunohistologically the expression of T-cell receptor (TCR)alpha chain on human tonsils and on T-cell lymphoma (T-ML) tissues using the avidin-biotin-peroxidase method. A murine monoclonal antibody alpha F1, specific for the constant region of the TCR alpha chain, was employed. On normal tonsil, alpha F1-positive cells were observed mainly in T-zones and germinal centers. In T-zones, the staining intensities varied markedly, with heavy staining evident in less than one fourth. In germinal centers, a proportion of stained cells showed a histiocytic pattern with small cytoplasmic projections. All the T-ML tissues expressed TCR alpha, whereas the staining intensities varied among cases and among lymphoma cells. No correlations were observed in the expressions of TCR alpha chain and other T-cell markers including CD3, CD4 and CD8.
Masunaga
A
A
Department of Pathology, University of Tokyo, Japan.
Baba
N
N
Kin
S
S
Mori
S
S
D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D017252:CD3 Complex; D015704:CD4 Antigens / Q000378:metabolism; D016827:CD8 Antigens; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D016410:Lymphoma, T-Cell, Cutaneous / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure*; D014066:Palatine Tonsil / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000378:metabolism*; D016693:Receptors, Antigen, T-Cell, alpha-beta; D012878:Skin Neoplasms / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure*
D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta