Bile acid N-acetylglucosaminides. Formation by microsomal N-acetylglucosaminyltransferases in human liver and kidney.
Bile acid N-acetylglucosaminyltransferase activity has been identified in microsomes from human liver and kidney. In both organs the transferases required UDP-N-acetylglucosamine as sugar donor and were mainly active towards ursodeoxycholic acid. Minor activities were observed towards amidated ursodeoxycholic, hyodeoxycholic and beta-muricholic acids. No N-acetylglucosaminidation was detectable with the major primary and secondary bile acids suggesting a specific requirement of the enzymes for bile acids containing 7 beta-or 6 alpha-hydroxyl groups. Kinetic parameters and other catalytic properties of liver and kidney microsomal N-acetylglucosaminyltransferase activities towards ursodeoxycholic acid are described.
Matern
H
H
Department of Internal Medicine III, Aachen University of Technology, FRG.
Bolz
R
R
Marschall
H U
HU
Sjövall
J
J
Matern
S
S
D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000378:metabolism; D001647:Bile Acids and Salts / Q000378:metabolism*; D005964:Glucosyltransferases / Q000378:metabolism*; D006801:Humans; D007668:Kidney / Q000201:enzymology*; D008861:Microsomes / Q000201:enzymology; D008862:Microsomes, Liver / Q000201:enzymology*; D017351:N-Acetylglucosaminyltransferases; D009928:Organ Specificity; D013379:Substrate Specificity
D001647:Bile Acids and Salts; D005964:Glucosyltransferases; D017351:N-Acetylglucosaminyltransferases; C037649:N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase; D000117:Acetylglucosamine