Metallopurpurins and light: effect on transplantable rat bladder tumors and murine skin.
Purpurins are modified chlorins with photodynamic properties. Their strong absorption in the red region of the visible spectrum makes them candidates for use in photodynamic cancer therapy. A series of metal derivatives of the free base purpurins have been synthesized and shown to cause tumor necrosis in transplantable tumors when exposed to visible light. In the following set of experiments, the effects of two metallo-derivatives (tin and zinc) of two purpurins, octaethylpurpurin (NT2) and etiopurpurin (ET2), and light on the N-[4-(5-nitro-2-furyl)-2-thiazolyl] formamide transplantable tumors in Fischer CDF(F344)/CrlBr rats were studied. The photodynamic activity was assessed by a short term assay using tumor dry weight 12 days after purpurin-PDT as a criterion of response. From these experiments it appears that SnET2 greater than SnNT2 greater than ZnET2 greater than ZnNT2 in photodynamic activity. SnET2 was further characterized by attempting to determine the time interval after systemic injection at which maximum therapeutic effect occurred. These studies shown that 24 h after metallopurpurin injection was the optimum time for treatment of tumors with visible light. In a final set of experiments, the effect of solar light on the skin of hairless mice injected with SnET2 was found to be much less injurious than with hematoporphyrin derivative.
Morgan
A R
AR
Department of Chemistry, University of Toledo, OH 43606.
Garbo
G M
GM
Keck
R W
RW
Eriksen
L D
LD
Selman
S H
SH
D000818:Animals; D005260:Female; D017324:Hematoporphyrin Derivative; D006415:Hematoporphyrins / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D008665:Metalloporphyrins / Q000494:pharmacology / Q000627:therapeutic use*; D051379:Mice; D008812:Mice, Hairless; D010778:Photochemotherapy; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D011916:Rats, Inbred F344; D012867:Skin / Q000187:drug effects* / Q000528:radiation effects; D013472:Sunlight; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy*
D006415:Hematoporphyrins; D008665:Metalloporphyrins; D011838:Radiation-Sensitizing Agents; D017324:Hematoporphyrin Derivative