[Cloning of grisin resistance gene gsr and the study of its functioning in Streptomyces griseus Kr. strain].
S. griseus Kr. is a commercial strain producing grisin, an antibiotic of the streptothricin group used as a feed additive. It was shown earlier that genetic instability of the strain was very high which was evident from a high frequency of nonreverting Grn- Grns mutants. With densitographic analysis of chromosomal DNA electrophoregrams and DNA-DNA hybridization it was revealed that the molecular basis of the genetic instability of the S. griseus strain was deletion of a DNA fragment about 20 kb in size containing a grisin resistance gene. The resistance gene designated as gsr was cloned to S. lividans TK 64 within the plasmid vector pIJ699. The restriction map of a cloned DNA fragment with a gsr gene was constructed and its similarity to that of a nat gene resistant to norseothricin, another streptothricin was observed. Introduction of a gsr gene within the multicopy plasmid pIJ699 into S. griseus 212, a highly productive strain synthesiing the antibiotic, led to an increase in its resistance and productivity. Proceeding from the preliminary data on possible linkage of a gsr gene and grisin biosynthesis genes, it appeared possible to use the cloned gene as a molecular probe in cloning the biosynthesis genes.
Sezonov
G V
GV
Kriugel'
G
G
Kudriashova
E A
EA
Kolibaba
L G
LG
Lomovskaia
N D
ND
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000494:pharmacology; D003001:Cloning, Molecular / Q000379:methods*; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial / Q000187:drug effects / Q000235:genetics*; D006824:Hybridization, Genetic / Q000235:genetics; D066298:In Vitro Techniques; D009154:Mutation / Q000187:drug effects / Q000235:genetics*; D013305:Streptomyces griseus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D013309:Streptothricins
D000900:Anti-Bacterial Agents; D003470:Culture Media; D004269:DNA, Bacterial; C005960:grisein; D013309:Streptothricins
Klonirovanie gena ustoĭchivosti k grizinu gsr i izuchenie ego funktsionirovaniia v shtamme-produtsente Streptomyces Kr.