Cell cultures enriched in oligodendrocytes from the central nervous system of trout in terms of phenotypic expression exhibit parallels with cultured rat Schwann cells.
Oligodendrocytes were isolated from the white matter of young trout by Percoll density centrifugation of enzymatically dissociated tissue and cultured on poly-D-lysine-coated petri dishes. Using antisera recognizing myelin-specific compounds of fish CNS (36K, IP2) up to 72% of the isolated cells could be identified as oligodendrocytes with an average yield of 4 x 10(6) cells per gram of wet tissue. Taken in culture, the cells rapidly regenerated their processes and soon acquired a morphology closely resembling mammalian oligodendrocytes in vitro. On the other hand, in terms of phenotypic expression, interesting parallels were revealed with the known in vitro behavior of Schwann cells: Galactocerebroside, which in mammalian oligodendrocytes is persistently expressed over longer periods of time in vitro, rapidly disappeared from the surface of cultured trout oligodendrocytes. In contrast, the fish CNS myelin glycoprotein IP2, which like IP1 is immunologically related to the major myelin product of Schwann cells, P0, was continuously expressed over several weeks in culture. Two other myelin protein constituents, 36K and IP1, transiently declined in vitro, but later on fully reappeared in the glial cells of trout. The present cell culture system offers an experimental model for studying in vitro the factors underlying oligodendroglial regeneration and remyelination in the fish CNS.
Jeserich
G
G
Department of Zoophysiology, University of Osnabrück, Federal Republic of Germany.
Rauen
T
T
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D007150:Immunohistochemistry; D009185:Myelin Proteins / Q000378:metabolism*; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D010641:Phenotype; D051381:Rats; D012487:Salmonidae / Q000502:physiology*; D012583:Schwann Cells / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D013045:Species Specificity; D014337:Trout / Q000502:physiology*
D009185:Myelin Proteins