title: Insect immunity. Characterization of a Drosophila cDNA encoding a novel member of the diptericin family of immune peptides.
abstract: Drosophila shows an immune response when challenged by injection of low doses of bacteria. To date, the molecules involved in this immune reaction have remained elusive, with the exception of cecropins (4-kDa antibacterial peptides initially isolated from the moth Hyalophora cecropia) for which three closely related genes have been characterized recently. We report the molecular cloning and sequencing of a cDNA from a library of immune Drosophila which encodes a novel member of the family of diptericins (9-kDa antibacterial peptides initially isolated from the fly Phormia terranovae). Transcripts for the Drosophila diptericin are detected 2 h after injection of bacteria. They are apparently derived from a single gene mapping at position 56 A on the right arm of the second chromosome. We discuss the existence of a distant relationship between the diptericins and two other groups of anti-bacterial insect proteins, the attacins, and the sarcotoxins II.
authors: Laboratoire de Biologie Générale de l'Université Louis Pasteur, Strasbourg, France.
authors: C
authors: C
authors: Wicker
authors: J M
authors: JM
authors: Reichhart
authors: D
authors: D
authors: Hoffmann
authors: D
authors: D
authors: Hultmark
authors: C
authors: C
authors: Samakovlis
authors: J A
authors: JA
authors: Hoffmann
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004175:Diptera / Q000235:genetics; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics* / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology; D007301:Insect Hormones / Q000235:genetics*; D019476:Insect Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family; D015345:Oligonucleotide Probes; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000900:Anti-Bacterial Agents; C054756:DptA protein, Drosophila; D029721:Drosophila Proteins; D007301:Insect Hormones; D019476:Insect Proteins; D015345:Oligonucleotide Probes; D004247:DNA