Examination of the potential mutagenicity and genotoxicity of some synthetic dyes.
The study was designed to investigate mutagenic and genotoxic properties of six synthetic dyes (Acid Green M, Acid Brown ZMSz, Acid Brown MSzCz, Anionic Black M, Anionic Brown ZM, Anionic Deep Red SM) obtained from All-Union Cancer Research Centre (AUCRC) in Moscow. To study mutagenicity and genotoxicity two short-term tests--Salmonella/microsome test using four strains of Salmonella-typhimurium TA97a, TA98, TA 100, TA102 and micronuclear test on mice were performed. The bacterial test was carried out with and without S9 mix fraction. The micronuclear test was conducted on mice Balb C with doses equal to 40 and 80% LD50 for male and 80% for female mice. Two of the studied dyes (Acid Green M. Anionic Brown ZM) proved to be direct and one (Anionic Deep Dark SM) indirect mutagens in the Salmonella/microsome assay. None of the studied compounds revealed a gentoxic activity in the micronucleus test. However, it was found that four dyes (Acid Brown ZMSz, Acid Brown MSzCz, Anionic Brown ZM and Anionic Deep Red SM) cause a significant decrease in the ratio of polychromatic to normochromatic erythrocytes in bone marrow of mice, which means that at doses used in the experiment they are toxic to erythrocyte series cells.
Przybojewska
B
B
Department of Occupational Carcinogenesis, Nofer Institute of Occupational Medicine, Lodz, Poland.
Barański
B
B
Spiechowicz
E
E
Wassilieva
L
L
Pylev
L N
LN
D000818:Animals; D004396:Coloring Agents / Q000633:toxicity*; D005260:Female; D066298:In Vitro Techniques; D007928:Lethal Dose 50; D008099:Liver / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015162:Micronucleus Tests; D008861:Microsomes / Q000187:drug effects; D009152:Mutagenicity Tests; D051381:Rats; D012475:Salmonella / Q000187:drug effects
D004396:Coloring Agents