Methodological approach to the characterization of diacylphosphatidyl cholines in rabbit lung lavage fluid by fast-atom bombardment mass spectrometry.
Strong evidence exists that the distribution of phospholipids in the lung is a function of the degree of adult respiratory distress syndrome. The capabilities of positive-ion fast-atom bombardment (FAB) mass spectrometry for the determination of the relative distribution of intact diacylphosphatidylcholine species in lung lavage fluid were investigated. Two different FAB matrices and two different isolation/purification procedures--extraction/thin layer chromatography--high performance liquid chromatography (TLC-HPLC)--have been evaluated. In addition the relative fatty acid composition of the diacylphosphatidylcholines was determined by negative-ion FAB mass spectrometry using the [RCOO]- fragment ion. These results were compared with those obtained by gas chromatographic determination of the fatty acid methylesters.
Allmaier
G
G
Institute for Analytical Chemistry, University of Vienna, Austria.
Schmid
E R
ER
Gasser
H
H
Strohmaier
W
W
Schlag
G
G
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D066298:In Vitro Techniques; D010713:Phosphatidylcholines / Q000737:chemistry*; D011817:Rabbits; D016339:Spectrometry, Mass, Fast Atom Bombardment
D010713:Phosphatidylcholines