Avian reticuloendotheliosis virus-transformed lymphoid cells contain multiple pp59v-rel complexes.
The v-rel oncogene of avian reticuloendotheliosis virus type T (REV-T) encodes a 59-kilodalton (kDa) phosphoprotein located principally in the cytosol of transformed lymphoid cells. All of the detectable pp59v-rel was present in high-molecular-weight complexes containing at least five cellular proteins (p124, p115, p75c-rel, p70hsc, and pp40). Antiserum was developed against the 40-kDa protein, the most abundant cellular protein associated with the complex. The 40-kDa phosphoprotein was complexed with pp59v-rel in REV-T-transformed lymphoid cell lines arrested at different stages of B-cell development as well as in lymphoid tumor cells and in fibrosarcomas. The half-life (8 h) of pp40 in REV-T-transformed lymphoid cells was the same as that of pp59v-rel. Antiserum against pp40 permitted the identification of two pp59v-rel complexes. The most abundant cytoplasmic complex contained approximately 75% of the pp59v-rel and all of the detectable pp40 in REV-T-transformed lymphoid cells. Twenty-five percent of the pp59v-rel was present in a minor complex that contained the majority of p75c-rel along with p115 and p124. In nuclear extracts of REV-T-transformed lymphoid cells, pp59v-rel was complexed with pp40. The two high-molecular-weight proteins (p115 and p124) and p75c-rel were not detected in the nuclear complex. In the cytosolic complexes, pp40 was heavily phosphorylated, whereas the nuclear form was much less extensively phosphorylated.
Davis
N
N
Department of Microbiology, University of Texas, Austin 78712.
Bargmann
W
W
Lim
M Y
MY
Bose
H
H
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002472:Cell Transformation, Viral; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera; D008214:Lymphocytes; D008970:Molecular Weight; D020572:Oncogene Proteins v-rel; D009857:Oncogenes; D010750:Phosphoproteins / Q000235:genetics*; D011863:Radioimmunoassay; D001356:Reticuloendotheliosis virus / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics*; D015682:Retroviridae Proteins, Oncogenic / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification
D007106:Immune Sera; D020572:Oncogene Proteins v-rel; D010750:Phosphoproteins; D015682:Retroviridae Proteins, Oncogenic