Human cells infected with retrovirus vectors acquire an endogenous murine provirus.
A 5.2-kilobase mouse RNA is expressed in human cells following infection with recombinant retroviruses propagated in mouse NIH 3T3 cells as psi-2 pseudotypes. This RNA is transcribed from a defective mink cell focus-forming provirus and copackaged into virions and integrated into human target cell DNA at a frequency comparable to that of the recombinant retrovirus genome.
Scadden
D T
DT
Howard Hughes Medical Institute, Boston, Massachusetts.
Fuller
B
B
Cunningham
J M
JM
D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D005822:Genetic Vectors; D006801:Humans; D009052:Leukemia Virus, Murine / Q000235:genetics*; D051379:Mice; D008908:Mink Cell Focus-Inducing Viruses / Q000235:genetics*; D008980:Moloney murine sarcoma virus / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D011533:Proviruses / Q000235:genetics*; D011995:Recombination, Genetic; D009053:Sarcoma Viruses, Murine / Q000235:genetics*; D014158:Transcription, Genetic; D014771:Virion / Q000235:genetics
D015345:Oligonucleotide Probes