The immunoglobulin lambda-like gene cluster (14.1, 16.1 and F lambda 1) contains gene(s) selectively expressed in pre-B cells and is the human counterpart of the mouse lambda 5 gene.
A human immunoglobulin (Ig)-related gene, covering approximately 8 kb, was isolated from a cosmid genomic library, by hybridization with a C lambda probe and with a lambda-like probe. This gene was identified as 14.1 It belongs to the human lambda-like cluster which is composed of three genes (14.1, 16.1 and F lambda 1) that do not rearrange. Sequence data indicate that 14.1 is organized similarly to the mouse lambda 5 gene. It contains three exons with lengths of 69, 38, and 106 codons as compared with 65, 38, and 106 for exons 1, 2, and 3 of mouse lambda 5, respectively. The corresponding homology values were 61, 66 and 75.5%. Using a 14.1 specific probe containing exon 1, we showed that this gene was selectively expressed in human pre-B cell lines. It is likely to encode a 213-amino acid lambda-like light chain that would associate with mu chains and play an important role in the early steps of B cell differentiation.
Schiff
C
C
Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France.
Bensmana
M
M
Guglielmi
P
P
Milili
M
M
Lefranc
M P
MP
Fougereau
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D003360:Cosmids; D005091:Exons; D005796:Genes; D005803:Genes, Immunoglobulin; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D007146:Immunoglobulin lambda-Chains / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D007146:Immunoglobulin lambda-Chains