[Extracorporeal survival and normothermia preservation of multiple ex vivo organ in rats].
A multiple organ block is composed of "en bloc" removed organs, connected by the vascular system, whose blood circulation is maintained by the heart, and oxygenation by the lungs under artificial ventilation. To develop the removal technique and the reanimation proceeding, and to validate this new physiological model, we used 70 Wistar rats. Some multiple organ blocks (heart-lungs, liver, pancreas, kidneys and bowel) functioned in vitro at 37 degrees C for more than 150 minutes with stable haemodynamics and preserved electrolyte and acid-base balances. Bile secretion and bilateral diuresis were present. Histopathological examination confirmed the integrity of these different organs. This physiological model could be used for the study of new preservation solutions.
Voiglio
E
E
Laboratoire de Chirurgie expérimentale et de Microchirurgie, INSERM U 80, CNRS, URA 1177, hôpital Edouard-Herriot, Lyon.
Gorry
F
F
Margonari
J
J
Faure
J L
JL
D000136:Acid-Base Equilibrium; D000818:Animals; D001831:Body Temperature; D005112:Extracorporeal Circulation; D008954:Models, Biological; D009926:Organ Preservation / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains; D012107:Research Design; D012122:Ventilators, Mechanical; D014882:Water-Electrolyte Balance
Maintien en survie extracorporelle et en normothermie de blocs multiviscéraux chez le rat.