title: U3 snRNPs and nucleolar development during oocyte maturation, fertilization and early embryogenesis in the mouse: U3 snRNA and snRNPs are not regulated coordinate with other snRNAs and snRNPs.
abstract: U3 small nuclear ribonucleic acids (snRNA) and U3 small nuclear ribonucleoprotein (snRNP), which are thought to be responsible for ribosomal RNA processing, are quantitated and localized during oocyte maturation, fertilization, and early embryogenesis in the mouse. On the basis of Northern blot and nuclease protection experiments, it is estimated that there are about 5 x 10(4) U3 snRNA molecules in an ovulated oocyte and in a two-cell embryo. This number then increases roughly 50-fold to 2.7 x 10(6) molecules per embryo by the blastocyst stage. At all stages of development U3 snRNP antigens colocalize with nucleoli, as defined by differential interference contrast microscopy and an antibody to a nucleolar epitope. The synthesis and distribution of U3 snRNA and U3 snRNP follow a pattern independent from other major U snRNPs and snRNAs.
authors: Integrated Microscopy Resource for Biomedical Research, University of Wisconsin, Madison 53706.
authors: R
authors: R
authors: Prather
authors: C
authors: C
authors: Simerly
authors: G
authors: G
authors: Schatten
authors: D R
authors: DR
authors: Pilch
authors: S M
authors: SM
authors: Lobo
authors: W F
authors: WF
authors: Marzluff
authors: W L
authors: WL
authors: Dean
authors: G A
authors: GA
authors: Schultz
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002466:Cell Nucleolus / Q000648:ultrastructure*; D005314:Embryonic and Fetal Development; D005260:Female; D005306:Fertilization; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D051379:Mice; D008813:Mice, Inbred ICR; D008970:Molecular Weight; D009865:Oocytes / Q000166:cytology* / Q000502:physiology; D012342:RNA, Small Nuclear / Q000032:analysis*; D012261:Ribonucleoproteins / Q000032:analysis*; D017411:Ribonucleoproteins, Small Nuclear; D015053:Zygote / Q000166:cytology*
chemical_list: D007106:Immune Sera; D012342:RNA, Small Nuclear; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear