5,8,11,14-eicosatetraynoic acid inhibits PC3 DNA synthesis and cellular proliferation, in part due to its 5'-lipoxygenase activity.
5,8,11,14-eicosatetraynoic acid (ETYA), a competitive analogue of arachidonic acid reversibly inhibited human prostate PC3 DNA synthesis at 4 h. This was not due to reduced cellular viability, as judged by several criteria, and resulted in reduced cell numbers at 72 h. Several less specific inhibitors of 5'-lipoxygenase, and A63162, a highly specific inhibitor, reduced labeling of DNA with 3H-thymidine. A23187, a calcium ionophore, reduced DNA synthesis, and ETYA rapidly increased [Ca2+]i measured with the Fura II technique. Comparison of the biochemical events evoked by ETYA and A63162 should identify those required for the downregulation of DNA synthesis.
Anderson
K M
KM
Department of Medicine, Rush Medical College, Chicago, Ill.
Harris
J E
JE
D015117:5,8,11,14-Eicosatetraynoic Acid / Q000494:pharmacology*; D016718:Arachidonic Acid / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured / Q000378:metabolism; D016861:Cyclooxygenase Inhibitors / Q000378:metabolism; D006801:Humans; D008084:Lipoxygenase / Q000378:metabolism*; D008297:Male; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism
D016861:Cyclooxygenase Inhibitors; D015117:5,8,11,14-Eicosatetraynoic Acid; D016718:Arachidonic Acid; D008084:Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases; D002118:Calcium