title: [A rapid diagnosis for periodontitis based on enzymatic activity of periodontopathic bacteria. Correlation between enzymatic activity, clinical periodontal parameters, and subgingival level of microorganisms].
abstract: There have been various laboratory methods for the microbiological diagnosis of periodontal disease. However, there have been some disadvantages in these methods. In this study of the application of a chair-side test for microbiological diagnosis, the activity of peptidases in periodontal pockets of patients was examined by using the assay system SK-013. SK-013 consists of synthetic substrates and is capable of rapidly (in 15 min) evaluating the activity of specific peptidase from Treponema denticola and Bacteroides species. Using SK-013, we evaluate the correlation between the enzymatic activity, clinical periodontal parameters and subgingival level of microorganisms, including phase contrast microscopy. We calculated the sensitivity and efficacy of SK-013 as a diagnostic indicator in the presence of Spirochetes and in periodontitis. A positive correlation were demonstrated between enzymatic activity, clinical periodontal parameters, the numbers of total cell count, Spirochetes, and M & S ratio. SK-013 was highly sensitive and efficacious (sensitivity: 92%, efficacy: 96%). We concluded that the assay system SK-013 is a useful chair-side method for diagnosing periodontal disease.
authors: Department of Periodontics, Showa University.
authors: T
authors: T
authors: Ohtake
authors: C
authors: C
authors: Sakurai
authors: C
authors: C
authors: Kurihara
authors: N
authors: N
authors: Minamizaki
authors: M
authors: M
authors: Kobayashi
authors: H
authors: H
authors: Miyashita
authors: K
authors: K
authors: Hasegawa
authors: T
authors: T
authors: Kobayashi
authors: E
authors: E
authors: Satoh
authors: N
authors: N
authors: Sugita
mesh_terms: D001439:Bacteroides / Q000201:enzymology; D001587:Benzoylarginine-2-Naphthylamide; D004796:Clinical Enzyme Tests / Q000379:methods*; D003773:Dental Plaque / Q000382:microbiology; D005188:False Negative Reactions; D005189:False Positive Reactions; D006801:Humans; D007202:Indicators and Reagents; D010447:Peptide Hydrolases / Q000032:analysis; D010518:Periodontitis / Q000175:diagnosis* / Q000382:microbiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013144:Spirochaetales / Q000201:enzymology; D014208:Treponema / Q000201:enzymology
chemical_list: D007202:Indicators and Reagents; D001587:Benzoylarginine-2-Naphthylamide; D010447:Peptide Hydrolases