title: Production of abiotic receptors by molecular imprinting of proteins.
abstract: When a protein is dissolved in a concentrated aqueous solution of a multifunctional organic compound, freeze-dried, and washed with an anhydrous organic solvent to remove the ligand, the resultant "imprinted" protein preparation binds up to 30-fold more of the template compound in anhydrous solvents than the nonimprinted protein in the same solvent (and both proteins in water). These artificial receptors exhibit marked ligand selectivity as well as stability in anhydrous media. This phenomenon of molecular imprinting, demonstrated for several unrelated proteins and ligands, may be helpful in the development of unique bioadsorbents and, potentially, new biocatalysts.
authors: Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139.
authors: L
authors: L
authors: Braco
authors: K
authors: K
authors: Dabulis
authors: A M
authors: AM
authors: Klibanov
mesh_terms: D002264:Carboxylic Acids / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism; D007700:Kinetics; D008024:Ligands; D008954:Models, Biological; D009113:Muramidase / Q000378:metabolism; D011107:Polylysine; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism
chemical_list: D002264:Carboxylic Acids; D006454:Hemoglobins; D008024:Ligands; D011506:Proteins; D011956:Receptors, Cell Surface; D011107:Polylysine; D012710:Serum Albumin, Bovine; D009113:Muramidase