title: [Purge of malignant cells from bone marrow by hematoporphyrin derivatives and light exposure in vitro].
abstract: During autologous bone marrow graft in treatment of malignant diseases, it is critical to purge malignant cells from the marrow. In the present study, the sensitivity to photodynamic inactivation of 3 leukemic cell lines was compared with their counterpart normal hematopoietic cells. After mouse leukemic L1210 cells were treated with a preparation of hematoporphyrin derivatives, YHpD, 10 micrograms/ml for 1 h and irradiated with black light (peak wavelength 395 nm, light intensity 0.6 mW/cm2) for 5 min, the survival rate of clonogenic cells decreased to less than 10%, while that of bone marrow granulocyte-macrophage progenitor cells (CFU-GM) in DBA/2 mice remained at nearly normal level (greater than 80%). Similar results were obtained when human leukemic HL-60 cells were compared to human CFU-GM and mouse leukemic L615 cells to CFU-GM in 615 strain mice. It is suggested that hematoporphyrin photoradiation may be useful for selectivity killing leukemic cells in bone marrow.
authors: Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
authors: C X
authors: CX
authors: Xu
authors: L
authors: L
authors: Lin
authors: Y Y
authors: YY
authors: Huang
authors: H Y
authors: HY
authors: Liu
authors: X R
authors: XR
authors: Wang
authors: Y X
authors: YX
authors: Wang
authors: B L
authors: BL
authors: Zhang
authors: X R
authors: XR
authors: Li
authors: Y H
authors: YH
authors: Hou
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D017324:Hematoporphyrin Derivative; D006414:Hematoporphyrin Photoradiation; D006415:Hematoporphyrins / Q000494:pharmacology*; D006801:Humans; D007939:Leukemia L1210 / Q000473:pathology*; D007942:Leukemia, Experimental / Q000473:pathology*; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology*; D051379:Mice; D008811:Mice, Inbred DBA; D008815:Mice, Inbred Strains; D010778:Photochemotherapy; D011838:Radiation-Sensitizing Agents; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D006415:Hematoporphyrins; D011838:Radiation-Sensitizing Agents; D017324:Hematoporphyrin Derivative