Mu gem3 as a tool to investigate the influence of chromosome supercoiling on gene expression in Escherichia coli K12.
We have developed a rapid method to investigate the influence of chromosome supercoiling on gene expression in Escherichia coli K12. This method exploits the ability of the gem3 mutant of the bacteriophage Mu, even in the prophagic state in immune cells, to induce relaxation of the host chromosome. The experiments can thus be performed under physiological conditions, and without the use of the drugs. In theory, this method can be applied to any bacterial gene. Here, we report the results obtained with four DNA replication and three cell division genes.
Bianchi
E
E
Centro Studi per gli Acidi Nucleici del CNR, Università di Roma Tor Vergata, Italy.
Ruberti
I
I
Ghelardini
P
P
Paolozzi
L
L
D010583:Bacteriophage mu / Q000235:genetics*; D002455:Cell Division; D002876:Chromosomes, Bacterial / Q000648:ultrastructure*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics / Q000648:ultrastructure; D004278:DNA, Superhelical / Q000235:genetics / Q000648:ultrastructure; D004926:Escherichia coli / Q000235:genetics / Q000648:ultrastructure*; D015964:Gene Expression Regulation, Bacterial; D009154:Mutation
D004269:DNA, Bacterial; D004278:DNA, Superhelical