Pseudointimal thrombogenicity changes in small arterial grafts.
Prosthetic grafts are rapidly covered by blood proteins after implantation. Cellular ingrowth and accumulation of interstitial proteins such as collagen complete the development of graft pseudointima. To investigate the effect of these changes on graft platelet reactivity, indium 111-labeled platelet accumulation on two grafts of differing porosity and thrombogenicity (polytetrafluoroethylene [PTFE] and Dacron) was measured 1, 7, and 21 days after graft implantation in canine carotid arteries. Pseudointima structure, weight, and collagen content were also determined at each measurement interval. In addition, platelet accumulation on carotid autografts and dissected carotid arteries was determined for comparison. Platelet accumulation on all grafts and arteries diminished significantly (p less than 0.05) between 1 and 7 days. Platelet accumulation on autografts and dissected arteries decreased further between 7 and 21 days and approached baseline. In contrast, platelet accumulation on Dacron grafts increased significantly (p less than 0.05) between 7 and 21 days to levels above those measured at 1 day, whereas platelet accumulation in PTFE grafts increased minimally and remained significantly below 1-day levels. A thick pseudointima that contained significant amounts of collagen developed in Dacron grafts during this time period, and a thin, incomplete pseudointima containing minimal collagen covered PTFE grafts. These differences in pseudointimal platelet reactivity and composition were associated with decreased patency of Dacron grafts (78%) compared to PTFE grafts (89%).
Seeger
J M
JM
Department of Surgery, University of Florida, Gainesville.
Borgeson
M
M
Lawson
G
G
D000818:Animals; D001158:Arteries / Q000637:transplantation; D001807:Blood Vessel Prosthesis; D004285:Dogs; D005260:Female; D008297:Male; D008854:Microscopy, Electron; D015539:Platelet Activation; D011093:Polyethylene Terephthalates; D011138:Polytetrafluoroethylene; D013927:Thrombosis; D014182:Transplantation, Autologous
D011093:Polyethylene Terephthalates; D011138:Polytetrafluoroethylene