Carboxyl-terminal deletion analysis of the Streptococcus mutans glucosyltransferase-I enzyme.
Sequential deletion of the carboxyl-terminal amino acids (including the six direct repeating units) of the glucosyltransferase-I (GTF-I) enzyme of Streptococcus mutans revealed differential effects on sucrase and GTF activities. Removal of all but one repeating unit resulted in a truncated enzyme with significant sucrase activity but no detectable GTF activity. These results are compatible with the presence of two functional domains in the enzyme.
Kato
C
C
Department of Pediatric Dentistry, University of Texas Health Science Center, San Antonio 78284.
Kuramitsu
H K
HK
D000595:Amino Acid Sequence; D002872:Chromosome Deletion; D005964:Glucosyltransferases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D013295:Streptococcus mutans / Q000201:enzymology / Q000235:genetics*
C036655:1,3-alpha-D-glucan synthase; D005964:Glucosyltransferases