title: The thymus-dependent nature of the murine antibody response to a monoclonal anti-idiotypic antibody to the Neisseria meningitidis serogroup C capsular polysaccharide.
abstract: Idiotype vaccines are proteins which may offer an alternative strategy for the conversion of a thymus-independent antigen into a thymus-dependent immunogen. To examine this question, we have studied the nature of the immune response to a monoclonal anti-idiotypic antibody, designated 6F9, which acts as a surrogate of Neisseria meningitidis serogroup C capsular polysaccharide, and compared this response to the nominal antigen, the meningococcal C-polysaccharide (MCP). BALB/c mice immunized with an optimal dose (100 micrograms) of 6F9 generate a specific anti-MCP IgG response which is maximal after 4 weeks. Secondary immunization with 6F9 results in a three- to five-fold increase in the specific IgG response. Mice given an optimal immunizing dose of MCP (5 micrograms) failed to generate an anti-MCP IgG response. No secondary response is detectable in mice immunized with MCP. Animals immunized with 6F9 and subsequently challenged with live meningococci group C show a significant anti-MCP IgG response. BALB/c nu/nu mice fail to generate an anti-MCP IgG antibody response to 6F9, while the nu/+ controls generate an anti-MCP IgG antibody titer 100 times that of the MCP-immunized mice. Neonatal mice that failed to respond to MCP developed early IgM and a subsequent IgG anti-MCP response after immunization with 6F9. These data demonstrate that the anti-idiotype 6F9, the combining site of which contains a surrogate image of the meningococcal group C capsular polysaccharide, evokes the responses expected of a T-dependent antigen.
authors: Department of Medicine, State University of New York, Buffalo 14215.
authors: M A
authors: MA
authors: Westerink
authors: P C
authors: PC
authors: Giardina
authors: A A
authors: AA
authors: Campagnari
authors: M A
authors: MA
authors: Apicella
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000276:immunology*; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D008807:Mice, Inbred BALB C; D009345:Neisseria meningitidis / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000276:immunology / Q000494:pharmacology*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D011135:Polysaccharides, Bacterial; C037676:meningococcal group C polysaccharide