Modulation of CALLA (CD10) antigen on cultured ALL (REH) cells: effect of various modulators.
Phorbol ester (TPA)-induced down-regulation of the common ALL (CALLA) antigen was studied by continuous flow immunocytometry with the aid of several CD10 monoclonal antibodies, including a new CD10 monoclonal antibody (DGH-10-1-A9), shown to be of IgG1 isotype, recognizing a 100 kDa cell surface protein and effectively inhibited by a series of reference CD10 monoclonal antibodies. The TPA-induced down-regulation of CALLA on REH cells was demonstrated with the aid of the following CD10 monoclonal antibodies: J-5, VIL-A1 and DGH-10-1-A9. No major modulations in cell surface expression of CALLA on REH cells were observed after induction with 1,25-(OH)2 vitamin D3, retinoic acid, recombinant interferon (IFN) alpha 2a and recombinant interleukin 2.
Chorváth
B
B
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Sedlák
J
J
Duraj
J
J
Plesková
I
I
Dubovský
P
P
Augustinová
M
M
Doxiadis
I
I
Grosse-Wilde
H
H
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000032:analysis / Q000235:genetics*; D000951:Antigens, Neoplasm / Q000032:analysis / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D015260:Neprilysin; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000276:immunology
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D015260:Neprilysin; D013755:Tetradecanoylphorbol Acetate