Expression and secretion of goat alpha-lactalbumin as an active protein in Saccharomyces cerevisiae.
An expression plasmid for goat alpha-lactalbumin in Saccharomyces cerevisiae, pSKA100, was constructed into a shuttle vector, pYG100, by inserting cDNA which encodes goat pre-alpha-lactalbumin and two-thirds of the 3'-non-coding region. The goat alpha-lactalbumin was expressed under the yeast glyceraldehyde 3-phosphate dehydrogenase (GPD) promoter and terminator of pYG100 and secreted in the growth medium for yeast as a precise mature protein, possessing specific activity essentially the same as that of authentic goat alpha-lactalbumin in lactose synthesis.
Takeda
S
S
Department of Industrial Chemistry, Faculty of Engineering, University of Tokyo, Japan.
Tamaki
E
E
Miura
K
K
Kumagai
I
I
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004271:DNA, Fungal / Q000096:biosynthesis; D015870:Gene Expression; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics*; D006041:Goats; D007768:Lactalbumin / Q000096:biosynthesis / Q000235:genetics*; D007785:Lactose / Q000096:biosynthesis; D007788:Lactose Synthase / Q000378:metabolism; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004271:DNA, Fungal; D011994:Recombinant Proteins; D007768:Lactalbumin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D007788:Lactose Synthase; D007785:Lactose