Epidemiology of Candida infection. II. Application of biochemical methods for typing of Candida albicans strains.
Biochemical profiles of 350 C. albicans isolates from five towns in Poland and from Freiburg in Germany were determined on the basis of nine biochemical tests of Odds and Abbott method. API 20 C AUX system and additionally a resistogram. The analysis of the strains according to Odds' and Abbotts's system showed that investigated strains can be typed into 9 profile codes of common biochemical patterns. There were some differences among the profiles according to their geographical origin and anatomical sources of the isolation. On the basis of the ability C. albicans strains to assimilate of carbon sources, 350 isolates were categorised into 13 separate auxotrophic profiles with the major one: 2,576,174 accounting for 81% of the total. The majority of the investigated isolates were susceptible to antifungal agents (83%). A disproportionate distribution of auxotrophic profiles limited the use of resistogram method and API 20 C AUX as systems for typing C. albicans strains. On the other hand, the method of Odds and Abbott provides valuable criteria for typing of C. albicans.
Budak
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Department of Mycology, Institute of Microbiology, Medical Academy, Kraków.
D000935:Antifungal Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D002176:Candida albicans / Q000145:classification* / Q000187:drug effects / Q000378:metabolism; D002177:Candidiasis / Q000453:epidemiology* / Q000382:microbiology; D050260:Carbohydrate Metabolism; D004352:Drug Resistance, Microbial; D006801:Humans; D010641:Phenotype
D000935:Antifungal Agents