Flow cytometric competitive binding assay for determination of actinomycin-D concentrations.
A single step, separation free competitive binding reaction between the fluorescent antibiotic mithramycin and actinomycin-D for common binding sites on DNA coated 10 microns diameter microspheres is described. The fluorescence of the microspheres is measured with a flowcytometer. In the presence of a constant amount of mithramycin, the microsphere fluorescence is inversely proportional to actinomycin-D concentration.
Saunders
G C
GC
Cell Biology Group, Los Alamos National Laboratory, New Mexico 87545.
Martin
J C
JC
Jett
J H
JH
Perkins
A
A
D001667:Binding, Competitive; D003609:Dactinomycin / Q000378:metabolism*; D005434:Flow Cytometry; D008863:Microspheres; D008926:Plicamycin / Q000378:metabolism
D003609:Dactinomycin; D008926:Plicamycin