title: Interleukin-2 receptor is similarly expressed by activated lymphocytes from patients on chronic hemodialysis and healthy subjects.
abstract: We studied peripheral blood mononuclear cells (PBMC) from 10 patients on chronic hemodialysis vs 10 healthy controls. T cells were counted by E rosette formation. Interleukin (IL)-2 receptor (IL-2R) carrier cells were identified by Dako-IL-2R, which was chosen as a monoclonal antibody that binds to the alpha chain of IL-2R without interrupting the cell machinery of mitogen-induced IL production. Fluorescein-tagged goat anti-rabbit IgG1* was used for subsequent cell staining, and cells were counted by FACS. Net numbers of PHA-activated IL-2R carrier cells were derived by subtracting appropriate background controls. The average number of these cells relative to numbers of total T cell population in uremic PBMC was statistically not different from healthy controls. Several defects in cellular activation associated with IL-2 mechanisms could be envisaged to account for the impaired proliferative response to PHA, manifested by uremic T cells.
authors: Nephrology Division, Assaf Harofeh Medical Center, Zerifin, Israel.
authors: D
authors: D
authors: Modai
authors: S
authors: S
authors: Berman
authors: Y
authors: Y
authors: Sheleg
authors: M
authors: M
authors: Cohn
authors: J
authors: J
authors: Weissgarten
authors: Z
authors: Z
authors: Averbukh
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000648:ultrastructure*; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D015375:Receptors, Interleukin-2 / Q000502:physiology*; D006435:Renal Dialysis; D012397:Rosette Formation; D050378:T-Lymphocytes, Regulatory / Q000166:cytology; D014511:Uremia / Q000097:blood*
chemical_list: D015375:Receptors, Interleukin-2