title: Induction of c-fos protein-like immunoreactivity in the rat and hamster pineal gland after the onset of darkness.
abstract: Induction of c-fos protein (FOS) after the onset of darkness was studied immunocytochemically in the rat and hamster pineal gland. The animals were kept on a 12:12 h light-dark cycle. Before the dark period no FOS staining was seen in either rat or hamster pineal cells. Five hours after the onset of darkness 342 +/- 18 pinealocytes/0.2 mm2 (mean +/- SD) displayed FOS-like immunoreactivity in the hamster pineal gland; in the rat pineal gland only 5 +/- 2 pinealocytes/0.2 mm2 showed a faint staining. Two hours later the density of FOS positive cells was decreased to 60 +/- 11/0.2 mm2 in the hamster but increased to 519 +/- 103/0.2 mm2 in the rat pineal gland. Three hours before the beginning of the light period no FOS positive cells were detected in either animal. Both the rat and hamster pineal gland showed a transient and temporally defined expression of c-fos protein in the middle of the dark period. This may be related to a more active functional state of pinealocytes, which is reflected in a peak of melatonin synthesis during the darkness.
authors: Department of Public Health, University of Tampere, Finland.
authors: J
authors: J
authors: Koistinaho
authors: G
authors: G
authors: Yang
mesh_terms: D000818:Animals; D002940:Circadian Rhythm; D006224:Cricetinae; D003624:Darkness; D007124:Immunoenzyme Techniques; D008297:Male; D008647:Mesocricetus; D010870:Pineal Gland / Q000166:cytology / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000096:biosynthesis*; D016760:Proto-Oncogene Proteins c-fos; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos