[In vitro processing of the artificial analog of beta-lipotropin].
The processing of the recombinant analogue of beta-lipotropin (beta-LHP) having 11 additional N-terminal amino acid residues and separated from the hormone by the processing signal, was investigated using rat adrenal secretory granule lysate as a test system of processing "in vitro". It was found that incubation of the beta-LPH analogue with secretory granule enzymes leads to its limited specific degradation with a release of native beta-endorphin. It is concluded that the additional N-terminal amino acids induced no qualitative changes in beta-LPH processing.
Kuz'menko
A P
AP
Fedosov
S A
SA
Golovin
S Ia
SIa
Mamaev
L V
LV
Korzhov
V A
VA
Il'ichev
A A
AA
Zagrebel'nyĭ
S N
SN
D000313:Adrenal Medulla / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D003594:Cytoplasmic Granules / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010957:Plasmids; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D001615:beta-Endorphin / Q000032:analysis; D008083:beta-Lipotropin / Q000096:biosynthesis* / Q000235:genetics
D011994:Recombinant Proteins; D001615:beta-Endorphin; D008083:beta-Lipotropin
Issledovanie protsessinga in vitro iskusstvennogo analoga beta-lipotropina.