title: Immobilization of alpha-human atrial natriuretic peptide on insoluble supports and affinity purification of specific antibodies from a polyclonal goat anti-alpha-human atrial natriuretic peptide serum.
abstract: alpha-Human atrial natriuretic peptide (alpha-hANP) was covalently coupled via single attachment onto two different insoluble matrices. Controlled-pore glass-alpha-hANP matrices were well suited for the purification of monospecific antibodies, whereas Enzacryl AA-alpha-hANP did not withstand the inevitable chemical and physical stresses during affinity purification.
authors: II. Medizinische Universitäts Klinik, Vienna, Austria.
authors: A
authors: A
authors: Missbichler
authors: R
authors: R
authors: Ferris
authors: E
authors: E
authors: Hartter
authors: W
authors: W
authors: Woloszczuk
authors: F
authors: F
authors: Pittner
mesh_terms: D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000918:Antibody Specificity; D009320:Atrial Natriuretic Factor / Q000276:immunology / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D006041:Goats / Q000276:immunology; D006801:Humans; D011863:Radioimmunoassay
chemical_list: D000906:Antibodies; D009320:Atrial Natriuretic Factor