Defective viral DNA in Epstein-Barr virus-associated oral hairy leukoplakia.
Defective Epstein-Barr virus (EBV) has a deleted and rearranged genome (termed het DNA) that disrupts latency and induces standard EBV to replicate in vitro. We used the polymerase chain reaction to detect, in 2 of 10 patient samples, the junction of abnormally juxtaposed EBV DNA fragments BamHI W and Z, a genomic rearrangement responsible for the biologic activity of het DNA. By sequence analysis, the junction in wild-type defective DNA appears to be similar but not identical to the recombination in the DNA of laboratory strain P3HR-1. The presence of this marker for het DNA in the epithelial lesions of two patients suggests a role for defective EBV in a human pathologic process.
Patton
D F
DF
Department of Hematology/Oncology, St Jude Children's Research Hospital, Memphis, Tennessee.
Shirley
P
P
Raab-Traub
N
N
Resnick
L
L
Sixbey
J W
JW
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D004259:DNA-Directed DNA Polymerase; D003673:Defective Viruses / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D005814:Genes, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D007972:Leukoplakia, Oral / Q000209:etiology / Q000382:microbiology*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping
D004279:DNA, Viral; D015345:Oligonucleotide Probes; D004259:DNA-Directed DNA Polymerase