Interleukin-1 receptor antagonist activity of a human interleukin-1 inhibitor.
Three interleukin-1 inhibitors have been purified to homogeneity from medium conditioned by human monocytes. Partial sequence analysis and digestion with N-glycanase indicate that these are glycosylation forms of a single protein. The protein binds to the interleukin-1 receptor but has no interleukin-1-like activity, even at very high concentrations, and is therefore a pure receptor antagonist.
Hannum
C H
CH
Synergen Inc., Boulder, Colorado 80301.
Wilcox
C J
CJ
Arend
W P
WP
Joslin
F G
FG
Dripps
D J
DJ
Heimdal
P L
PL
Armes
L G
LG
Sommer
A
A
Eisenberg
S P
SP
Thompson
R C
RC
D000581:Amidohydrolases; D000595:Amino Acid Sequence; D001667:Binding, Competitive; D002478:Cells, Cultured; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D015232:Dinoprostone / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000037:antagonists & inhibitors*; D008969:Molecular Sequence Data; D009000:Monocytes / Q000378:metabolism*; D010446:Peptide Fragments; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D011506:Proteins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D011971:Receptors, Immunologic / Q000037:antagonists & inhibitors* / Q000378:metabolism; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology; D012795:Sialoglycoproteins
C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D010446:Peptide Fragments; D011506:Proteins; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins; D012795:Sialoglycoproteins; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D015232:Dinoprostone