title: Effect of bacterial toxins on human B cell activation. I. Mitogenic activity of pertussis toxin.
abstract: Pertussis toxin (PT) was found to elicit an increased thymidine uptake in resting B lymphocytes purified from human peripheral blood. A significant mitogenic effect was detected for toxin concentrations greater than 100 ng/ml (1nM) and a plateau of stimulation was reached at 1000 ng/ml (10 nM). B cell blasts, activated by a first signal such as Staphylococcus aureus Cowan I or insolubilized anti-mu chain antibody, were also stimulated to DNA synthesis by PT in the same range of concentrations. At lower sub-mitogenic concentrations, the toxin potentiated the response to the low-molecular weight B cell growth factor (LMW-BCGF or 12-kDa BCGF), a progression factor for activated B cells. The "A" or catalytic subunit was devoid of any activity on B cells, suggesting the stimulatory effect of the toxin might be associated with the binding or "B" subunit, as it has been shown for T cells. This hypothesis was strengthened by the observation that, as in T cell, the whole toxin but not the "A" promoter, was able to induce calcium influx in these cells. In addition, the purified "B" oligomer alone was found to promote DNA synthesis in B cells. Finally, a fragment of the soluble cleaved form of the CD23 molecule (Fc epsilon RII) could be involved in the process of PT mitogenicity for B cells.
authors: U 196 INSERM, Institut Curie, Paris, France.
authors: J P
authors: JP
authors: Kolb
authors: E
authors: E
authors: Genot
authors: E
authors: E
authors: Petit-Koskas
authors: N
authors: N
authors: Paul-Eugene
authors: B
authors: B
authors: Dugas
mesh_terms: D000944:Antigens, Differentiation, B-Lymphocyte / Q000378:metabolism; D001402:B-Lymphocytes / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000502:physiology; D006801:Humans; D015847:Interleukin-4 / Q000502:physiology; D007399:Interphase / Q000187:drug effects; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects; D008934:Mitogens; D037342:Pertussis Toxin; D011961:Receptors, Fc / Q000378:metabolism; D017455:Receptors, IgE; D013601:T-Lymphocytes / Q000187:drug effects; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D008934:Mitogens; D011961:Receptors, Fc; D017455:Receptors, IgE; D010566:Virulence Factors, Bordetella; D015847:Interleukin-4; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins; D002118:Calcium