title: Incorporation of 7-deaza dGTP during the amplification step in the polymerase chain reaction procedure improves subsequent DNA sequencing.
abstract: An improved method for sequencing human genomic DNA amplified by the polymerase chain reaction (PCR) procedure is described. The portion of the genome investigated is the 383 nucleotide-long exon 2 of the human antithrombin III gene. Incorporation of the analogue of dGTP, 7-deaza-2'-deoxyguanosine-5'-triphosphate, during the amplification of exon 2 by PCR allowed for the elimination of recurrent artifacts obtained during sequencing of the amplified DNA by the dideoxyribonucleotide chain termination method.
authors: Department of Pathology, McMaster University, Hamilton, Ontario, Canada.
authors: F
authors: F
authors: Fernandez-Rachubinski
authors: B
authors: B
authors: Eng
authors: W W
authors: WW
authors: Murray
authors: M A
authors: MA
authors: Blajchman
authors: R A
authors: RA
authors: Rachubinski
mesh_terms: D000990:Antithrombin III / Q000235:genetics; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA; D006147:Guanine / Q000031:analogs & derivatives* / Q000737:chemistry; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D006147:Guanine; D000990:Antithrombin III; D004247:DNA; C066856:7-deazaguanine