Nucleotide sequence and some biological properties of the NS gene of a newly isolated influenza B virus mutant which has a long carboxyl terminal deletion in the NS1 protein.
By a heterotypic cross between influenza viruses A/WSN (H1N1) and B/Yamagata/1/73, we obtained a mutant of B/Yamagata (AWBY-234), which expressed a greatly truncated NS1 protein with molecular weight of 13,500. Direct sequencing of the NS gene of the mutant revealed a deletion of a single uridine base at the position 310, 311, or 312 of the plus sense RNA, giving rise to a new stop codon at the position 314-316. The resulting NS1 protein was predicted to be composed of only 90 amino acids, compared with 281 with the NS1 of the wild-type B/Yamagata. AWBY-234 grew normally and induced a typical cytopathic effect in infected MDCK cells much earlier after infection than did the wild-type B/Yamagata. A single gene reassortant in which the NS gene of AWBY-234 was transferred to B/Lee/40 inherited these characteristics from AWBY-234 parent. The single gene reassortant, but not the control reassortant, contained a significant amount of defective particles which can complement each other to produce infectious virus.
Tobita
K
K
Department of Virology, Jichi Medical School, Tochigi-Ken, Japan.
Tanaka
T
T
Odagiri
T
T
Tashiro
M
M
Feng
S Y
SY
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000032:analysis / Q000235:genetics*; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D009981:Influenza B virus / Q000235:genetics* / Q000254:growth & development; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009154:Mutation; D012367:RNA, Viral / Q000235:genetics*; D014758:Viral Core Proteins / Q000032:analysis / Q000235:genetics*; D017361:Viral Nonstructural Proteins
D012367:RNA, Viral; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins