A determination of the radial coordinate of Tyr-69 in F-actin using fluorescence energy transfer.
Fluorescence energy transfer was measured between Tyr-69 residues in an F-actin filament using 5-dimethylaminonaphthalene-1-sulfonyl chloride (DNS-Cl) as a fluorescence energy donor and 4-dimethylaminoazobenzene-4-sulfonyl chloride (DABSYL-Cl) as the acceptor. Both labels are covalently attached to Tyr-69 residues in an F-actin filament. Taking the helical structure of the F-actin filament into consideration, the radial coordinate of Tyr-69 was calculated to be in the range from 2.0 nm to 4.0 nm.
Miki
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Department of Anatomy, University of Sydney, N.S.W., Australia.
dos Remedios
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D000199:Actins / Q000737:chemistry*; D000818:Animals; D003619:Dansyl Compounds; D004735:Energy Transfer; D005453:Fluorescence; D007202:Indicators and Reagents; D011817:Rabbits; D013050:Spectrometry, Fluorescence; D014443:Tyrosine / Q000737:chemistry*; D004124:p-Dimethylaminoazobenzene / Q000031:analogs & derivatives
D000199:Actins; D003619:Dansyl Compounds; D007202:Indicators and Reagents; D014443:Tyrosine; C010572:4-N,N-dimethylaminoazobenzene-4'-sulfonyl chloride; D004124:p-Dimethylaminoazobenzene; C029038:dansyl chloride