[Apatite-collagen complex. Preparation of a new apatite-collagen complex].
A new apatite-collagen complex was prepared in calcium beta-glycerophosphate solutions at pH 8.50. For this preparation, reconstituted type I collagen was cross-linked with phosvitin in the presence of alkaline phosphatase by use of a cross-linking agent of dimethyl suberimidate. After two weeks of immersion in daily-renewed solution of calcium beta-glycerophosphate, the complex contained apatite approximately two times the modified collagen in weight. When viewed in a scanning electron microscope, needle-like crystals precipitated densely on the collagen fibrils. However, in some portion of the complex, dot-like precipitate was observed as well. X-ray diffraction and IR analyses of the complex suggested that the apatite precipitated on the collagen fibrils was very similar to bone mineral in two aspects, crystallinity and carbonate content.
Horiguchi
T
T
Department of Dental Materials & Technology, School of Dentistry, Asahi University.
Doi
Y
Y
Kim
S H
SH
Wakamatsu
N
N
Adachi
M
M
Imura
S
S
Hayashi
K
K
Gyotoku
T
T
D001031:Apatites / Q000138:chemical synthesis*; D003094:Collagen; D003432:Cross-Linking Reagents; D003460:Crystallization; D008855:Microscopy, Electron, Scanning; D010750:Phosphoproteins; D013055:Spectrophotometry, Infrared; D014961:X-Ray Diffraction
D001031:Apatites; D003432:Cross-Linking Reagents; D010750:Phosphoproteins; D003094:Collagen