title: Bacteriophage Mu sites and functions involved in the inhibition of lambda::mini-Mu growth.
abstract: To better understand the nature of the mini-Mu-directed process which results in inhibition of lambda::mini-Mu growth we characterized spontaneous deletion mutants of the lambda::mini-Mu phage. On the basis of analysis of the deletion endpoints, mini-Mu replication functions, and integration and inhibition properties, the lambda::mini-Mu deletion mutants were divided into five classes which define the Mu sites and functions involved in lambda::mini-Mu growth inhibition. Class 1 mutants, which still exhibit lambda::mini-Mu growth inhibition, collectively delete all the Mu late functions encoded by the mini-Mu. Class 2 and 5 mutants, which show cis-dominant defects in inhibition and integration, delete the right and left mini-Mu attachment sites, respectively. Phages of Classes 3 and 4, which delete the Mu B or A and B genes, respectively, show recessive defects in growth inhibition. The properties of these mutants define the Mu replication functions, A and B, and the Mu attachment sites as essential for the inhibition of lambda::mini-Mu growth. The observation that the sites and functions essential for Mu replication also have requisite roles in the inhibition of lambda::mini-Mu growth suggests that inhibition results from mini-Mu-promoted replicative interference of lambda::mini-Mu development.
authors: Department of Bacteriology, University of Wisconsin, Madison 53706.
authors: A C
authors: AC
authors: Glasgow
authors: J L
authors: JL
authors: Miller
authors: M M
authors: MM
authors: Howe
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics* / Q000254:growth & development; D010583:Bacteriophage mu / Q000235:genetics* / Q000254:growth & development; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005816:Genetic Complementation Test; D005838:Genotype; D008242:Lysogeny; D009154:Mutation; D015183:Restriction Mapping
chemical_list: D004279:DNA, Viral