title: Preservation of ruminal bacterium capsules by using lysine in the electron microscopy fixative.
abstract: Ruminal bacteria from axenic cultures of Ruminococcus flavefaciens FD1, Butyrivibrio fibrisolvens 49, and bacterial types from the ruminal ecosystem that were fixed with 50 mM lysine (l-lysine hydrochloride) added to glutaraldehyde had better-preserved capsules and extracellular material than bacteria fixed without lysine.
authors: Richard B. Russell Agricultural Research Center, U.S. Department of Agriculture, Athens, Georgia 30613.
authors: D E
authors: DE
authors: Akin
authors: L L
authors: LL
authors: Rigsby
mesh_terms: D000818:Animals; D001419:Bacteria / Q000648:ultrastructure*; D001431:Bacteriological Techniques; D005404:Fixatives; D006089:Gram-Negative Anaerobic Bacteria / Q000648:ultrastructure; D008239:Lysine; D008854:Microscopy, Electron; D010459:Peptococcaceae / Q000648:ultrastructure; D012417:Rumen / Q000382:microbiology*
chemical_list: D005404:Fixatives; D008239:Lysine