[Isoelectric focusing of mixed saliva proteins in individuals with HGH caries prevalence].
The protein content of mixed saliva was analysed in patients with low, mid and high caries prevalence by isoelectric focusing in different pH gradient on Servalyt plates. Determination of the protein fractions in pH-3-6.gradient points out a significant increase of glycoprotein fractions of pH = 4.7. The most basic fractions of this range (pH = 5.9) are disappearing within the high caries prevalence. Isoelectric focusing in pH-6-9 gradient points out that with the increase of caries prevalence there is an increase in protein fractions of pH = 8.3. Protein fractions of lysosomal prigin, pH = 10.65, are disappearing with the increase of caries prevalence in all analysed specimens. IEF method on Servalyt plates and polyacrylamid gel enables analysis of the Ca-precipitable acid and base like glyco-and phosphoprroteins (pHi = 4 and 8.3) which are of significance in maintaining physiological properties of the soft and hard teeth tissues.
Mirković
S
S
University School of Dental Medicine, Belgrade.
Popović
V
V
Lekić
P
P
D000328:Adult; D003731:Dental Caries / Q000276:immunology / Q000378:metabolism*; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D012471:Salivary Proteins and Peptides / Q000032:analysis*
D006023:Glycoproteins; D012471:Salivary Proteins and Peptides
Izoelektricno fokusiranje proteina mesovite pljuvacke osoba sa frekventnim karijesom.