[The diagnostic effectiveness of erythrocyte immunoreagents made from different Pseudomonas aeruginosa antigens].
The diagnostic effectiveness of the passive hemagglutination (PHA) tests with the use of erythrocyte diagnostica (ED based on exotoxin A (ETA) and poly- and monovalent ED based on lipopolysaccharides (LPS) isolated from 5 most widespread P. aeruginosa serogroups was compared. 97 patients with different purulent septic diseases and 100 practically healthy adult donors were examined. The treatment of serum samples with 2-mercaptoethanol decreased the diagnostic effectiveness of serological examination. The simultaneous determination of the activity of antibodies to ETA and LPS essentially increased the number of positive results of the PHA tests in patients with bacteriologically confirmed P. aeruginosa infection, but not in patients from whom other bacteria were isolated. Considering the sensitivity and specificity of serological examination, as well as the decrease of the volume of necessary work and the consumption of immunoreagents, the optimum test conditions proved to be ensured by the use of two ED: LPS-based polyvalent ED and ETA-based ED.
Egizova
S A
SA
Deriabin
P N
PN
Karal'nik
B V
BV
Moroz
A F
AF
Aleksandrova
I A
IA
Aleksandrov
A D
AD
Slastikhin
B A
BA
Roshchina
N V
NV
D000942:Antigens, Bacterial / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology*; D005069:Evaluation Studies as Topic; D005098:Exotoxins / Q000276:immunology; D006386:Hemagglutination Tests; D006801:Humans; D007202:Indicators and Reagents; D008070:Lipopolysaccharides / Q000276:immunology; D011512:Proteus Infections / Q000175:diagnosis; D011552:Pseudomonas Infections / Q000175:diagnosis; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D012698:Serologic Tests / Q000379:methods; D013203:Staphylococcal Infections / Q000175:diagnosis
D000942:Antigens, Bacterial; D005098:Exotoxins; D007202:Indicators and Reagents; D008070:Lipopolysaccharides
Diagnosticheskaia éffektivnost' éritrotsitarnykh immunoreagentov iz razlichnykh antigenov Pseudomonas aeruginosa.