Cytogenetic profile of in vitro spontaneously transformed and chemical tumor-derived cell lines related to the mouse lung alveologenic carcinoma.
The karyotypes for a unique series of cloned untransformed and spontaneously and urethan-transformed mouse lung alveologenic carcinoma cell lines were compared by G-banding. All cell lines exhibited altered chromosome number accompanied by chromosomal aberrations. Both spontaneously and chemically transformed lines contained a higher proportion of cells carrying double minutes, short-arm chromosomes with no distinct banding pattern, and Robertsonian translocations between a variety of chromosomes. No common karyotypic abnormalities were observed among any of the transformed lines. These data suggest that enhanced genomic instability may lead to a critical subchromosomal molecular event(s) in generation of both spontaneously and chemically derived mouse lung alveologenic carcinoma.
Markus
I
I
Carcinogenesis Research Unit, School of Pathology, University of New South Wales, Kensington, Australia.
Smith
G J
GJ
D002282:Adenocarcinoma, Bronchiolo-Alveolar / Q000235:genetics* / Q000473:pathology; D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002871:Chromosome Banding; D002875:Chromosomes / Q000648:ultrastructure; D002999:Clone Cells; D007621:Karyotyping; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D051379:Mice; D014407:Tumor Cells, Cultured / Q000166:cytology