A regular pattern of two types of 100-residue motif in the sequence of titin.
Titin is the largest polypeptide yet described (relative molecular mass approximately 3 x 10(6); refs 1, 2) and an abundant protein of striated muscle. Its molecules are string-like and in vivo span from the M to Z-lines. I-band regions of titin are thought to make elastic connections between the thick filament and the Z-line, thereby forming a third type of sarcomere filament. These would centre the A-band in the sarcomere and provide structural continuity in relaxed myofibrils. The A-band region of titin seems to be bound to the thick filament, where it has been proposed to act as a 'molecular ruler' regulating filament length and assembly. Here, we show that partial titin complementary DNAs encode a regular pattern of two types of 100-residue motif, each of which probably folds into a separate domain type. Such motifs are present in several evolutionarily divergent muscle proteins, all of which are likely to interact with myosin. One or both of the domain types is therefore likely to bind to myosin.
Labeit
S
S
European Molecular Biology Laboratory, Heidelberg, FRG.
Barlow
D P
DP
Gautel
M
M
Gibson
T
T
Holt
J
J
Hsieh
C L
CL
Francke
U
U
Leonard
K
K
Wardale
J
J
Whiting
A
A
D000595:Amino Acid Sequence; D000818:Animals; D015139:Blotting, Southern; D002352:Carrier Proteins / Q000235:genetics; D003001:Cloning, Molecular; D064211:Connectin; D004247:DNA / Q000235:genetics; D015723:Gene Library; D005796:Genes; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D011494:Protein Kinases; D011817:Rabbits; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D002352:Carrier Proteins; D064211:Connectin; D009124:Muscle Proteins; D004247:DNA; D011494:Protein Kinases