Characterization of a cAMP-regulated enhancer-binding protein.
The transcription regulation of many hormone genes is modulated by intracellular second messengers such as cAMP. The cAMP response element binding protein, CREB, binds to the 8 base pair CRE enhancer, TGACGTCA, that is found in the 5'-flank of certain genes including those for somatostatin and the alpha-subunit of human chorionic gonadotropin. The recent characterization of CREB and CREB-related cDNA clones, combined with Southwesterns and Northern blot analyses, reveals a family of transcription factors that dimerize via a leucine zipper motif and bind to the CRE through positively charged basic regions. The CREB cDNA encoding a 327 residue protein is transcriptionally activated via phosphorylation by protein kinases, including the cAMP-dependent protein kinase-A.
Habener
J F
JF
Laboratory of Molecular Endocrinology, Massachusetts General Hospital, Boston 02114.
Meyer
T E
TE
Yun
Y
Y
Waeber
G
G
Hoeffler
J P
JP
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017362:Cyclic AMP Response Element-Binding Protein; D004247:DNA / Q000032:analysis*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000502:physiology; D004742:Enhancer Elements, Genetic / Q000502:physiology*; D005260:Female; D006801:Humans; D008954:Models, Biological; D008969:Molecular Sequence Data; D010766:Phosphorylation; D010920:Placenta / Q000378:metabolism; D011494:Protein Kinases / Q000502:physiology*; D014157:Transcription Factors / Q000502:physiology*
D017362:Cyclic AMP Response Element-Binding Protein; D004268:DNA-Binding Proteins; D014157:Transcription Factors; D004247:DNA; D011494:Protein Kinases