The frequency of mouse spleen dendritic cells which present alloantigens or ovalbumin to primed T lymphocytes is equal.
We have used limiting dilution analysis to compare the frequency of dendritic cells (DC) which present endogenous alloantigens with that which present an exogenous protein antigen to T lymphocytes. Spleen DC present alloantigens or ovalbumin to primed T lymphocytes with equal frequency, showing that DC are equipotent for presenting endogenous and exogenous antigens. Also, antigen-presenting cell (APC) frequencies among DC were compared with other APC populations. DC were enriched about 1000-fold for APC compared to unfractionated spleen cells.
Goodacre
J A
JA
Department of Medicine, School of Pathological Sciences, University of Newcastle upon Tyne, England.
Johnstone
R
R
Atherton
M C
MC
Robinson
J H
JH
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007519:Isoantigens / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000949:Histocompatibility Antigens Class II; D007519:Isoantigens; D010047:Ovalbumin