title: [Inhibition of casein kinase type 2 activity by formation of complexes with mRNA in vivo].
abstract: It is known that casein kinase 2 possesses, besides the protein kinase, an RNA-binding activity. Using ligand blotting it has been demonstrated that the both activities are localized on the alpha- and alpha'-subunits of the enzyme. Casein kinase 2 is suppressed in vitro by polyuridylic acid. A part of the intracellular pool of casein kinase 2 is found in the informosomes. The informosomes and free proteins were separated by centrifugation in a sucrose density gradient, and each fraction was incubated with casein and [gamma-32P]ATP. The informosome-bound protein kinase is completely inhibited, while the free protein kinases heavily phosphorylate casein. It is concluded that the activity of casein kinase 2 can be regulated by the reversible formation of complexes with RNA.
authors: K V
authors: KV
authors: Kandror
authors: A S
authors: AS
authors: Stepanov
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D047388:Casein Kinases; D010766:Phosphorylation; D011485:Protein Binding; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins; D011896:Rana temporaria
chemical_list: D002352:Carrier Proteins; D047428:Protein Kinase Inhibitors; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011494:Protein Kinases; D047388:Casein Kinases
vernacular_title: Ingibirovanie aktivnosti kazeinovoĭ kinazy tipa 2 za schet obrazovaniia kompleksov.