title: Staphylococcal enterotoxin A induced interferon (IFN)-gamma production in spleen cells from BCG-immunized mice: the IFN production is dependent on leukotriene C4 but not dependent on interleukin 2.
abstract: In our previous paper, we showed that IFN was induced in sera by injection of staphylococcal enterotoxin A (SEA) in Bacillus Calmette-Guérin (BCG) immunized C57BL/6 (B6) mice. In analyzing the phenomenon in vitro, we showed that SEA induced IFN-gamma in the supernatant of the spleen cell culture from BCG immunized B6 mice and that leukotriene C4 (LTC4) from BCG activated macrophages in the spleen was involved in the IFN production from Ly 1+ T cells. On the other hand, interleukin-2 (IL-2) has reported to play an important role in the regulation of synthesis of IFN-gamma by T cells. In the present study, we examined whether IL-2 is involved in SEA-induced IFN production. The result showed that the SEA-induced IFN-gamma production was observed in spite of suppression of SEA-induced IL-2 production in spleen cells from BCG-immunized B6 mice. On the contrary, the depressed IFN production was observed in spite of high SEA-induced IL-2 production in spleen cells from their control mice. On the other hand, LTC4 production was 8 times higher in spleen cells from BCG-immunized B6 mice, high producer of SEA-induced IFN, than in that from BCG-immunized C3H mice, the low producer. We also observed that the IFN and the LTC4 production of spleen cells from BCG-immunized B6 mice was suppressed in the presence of caffeic acid and nordihydroguaiaretic acid, non-specific lipoxygenase inhibitors, and that LTC4 augmented the IFN production of normal B6 mouse spleen cells in the presence of 2-mercaptoethanol. Therefore, involvement of LTC4 rather than of IL-2 was supported in our experimental system.
authors: Department of Internal Medicine, Tomakoraai City General Hospital, Japan.
authors: K
authors: K
authors: Kato
authors: K
authors: K
authors: Shirosita
authors: S
authors: S
authors: Kurosawa
authors: N
authors: N
authors: Mizukoshi
authors: K
authors: K
authors: Yamamoto
authors: I
authors: I
authors: Azuma
authors: H
authors: H
authors: Okuyama
authors: J
authors: J
authors: Nishihira
mesh_terms: D000818:Animals; D001500:BCG Vaccine / Q000276:immunology*; D002109:Caffeic Acids / Q000494:pharmacology; D004768:Enterotoxins / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D007114:Immunization; D007371:Interferon-gamma / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000502:physiology*; D016859:Lipoxygenase Inhibitors; D009637:Masoprocol / Q000494:pharmacology; D008623:Mercaptoethanol / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H / Q000276:immunology; D008810:Mice, Inbred C57BL / Q000276:immunology; D013189:SRS-A / Q000096:biosynthesis / Q000502:physiology*; D013154:Spleen / Q000187:drug effects* / Q000378:metabolism; D013268:Stimulation, Chemical
chemical_list: D001500:BCG Vaccine; D002109:Caffeic Acids; D004768:Enterotoxins; D007376:Interleukin-2; D016859:Lipoxygenase Inhibitors; D013189:SRS-A; C026066:enterotoxin A, Staphylococcal; D008623:Mercaptoethanol; D009637:Masoprocol; D007371:Interferon-gamma; C040048:caffeic acid