Protein analysis by electrospray ionization mass spectrometry.
The applicability of electrospray ionization (ESI) mass spectrometry to protein analyses has been studied. The molecular weight of hen egg lysozyme (HEL) was determined with an accuracy of +/- 2 u. The choice of solvents and additives in sample preparations was important to achieve high sensitivity as well as high precision of molecular weight measurements.
Ishikawa
K
K
National Chemical Laboratory for Industry, Ibaraki, Japan.
Niwa
Y
Y
D000418:Albumins / Q000737:chemistry; D000818:Animals; D002256:Carbonic Anhydrases / Q000737:chemistry; D002417:Cattle; D002645:Chickens; D004531:Eggs / Q000032:analysis; D005069:Evaluation Studies as Topic; D007328:Insulin / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D008970:Molecular Weight; D009113:Muramidase / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*
D000418:Albumins; D007328:Insulin; D011506:Proteins; D009113:Muramidase; D002256:Carbonic Anhydrases