title: Enteric adenovirus 41 (Tak) requires low serum for growth in human primary cells.
abstract: It had been postulated that due to lack of growth of enteric adenovirus 41 (Ad41) on human primary cells and its growth on Graham-293 cells there was a defect in the Ad41 E1A region. However, we found as a result of careful evaluation of Ad41 growth on several primary cell lines (HEK, WI-38, or Detroit 551) that efficient viral multiplication is possible if the serum concentration in the medium used postinfection (p.i.) is kept between 0.2 and 1%. In contrast, only slight growth of Ad41 occurs in infected cells maintained in 5% serum and virtually no viral replication is found in infected cells cultivated in medium with 10% serum. The serum inhibitory effect appears limited to primary cells because no difference in Ad41 replication, as assayed by accumulation of Ad41 DNA, was found in infected continuous cell lines (HEp-2, 293) cultivated p.i. in either 1 or 10% FBS. Also, this effect appears specific for enteric adenoviruses, such as Ad41, since conventional adenoviruses, such as Ad5, grow well in both 1 and 10% FBS. The above results show that Ad41 can grow in a variety of primary cell lines, under specific culture conditions. In addition, we found that Ad41-infected primary cells grown in medium containing 0.2% serum had an increase in synthesis of the 70-kDa heat shock protein (HSP70) at about 6 hr p.i. and also Ad41 was able to complement the Ad5 E1A deletion mutant dl312. These results show that the E1A function of Ad41 is not impaired in infected cells.
authors: Department of Microbiology, Immunology and Parasitology, Louisiana State University Medical Center, New Orleans 70112-1393.
authors: D
authors: D
authors: Pieniazek
authors: N J
authors: NJ
authors: Pieniazek
authors: D
authors: D
authors: Macejak
authors: R B
authors: RB
authors: Luftig
mesh_terms: D017456:Adenovirus Early Proteins; D000258:Adenovirus Infections, Human / Q000188:drug therapy; D000260:Adenoviruses, Human / Q000254:growth & development*; D001769:Blood; D002478:Cells, Cultured; D004261:DNA Replication; D004279:DNA, Viral / Q000378:metabolism*; D006367:HeLa Cells; D006360:Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D009154:Mutation; D009856:Oncogene Proteins, Viral / Q000235:genetics; D014779:Virus Replication
chemical_list: D017456:Adenovirus Early Proteins; D004279:DNA, Viral; D006360:Heat-Shock Proteins; D009856:Oncogene Proteins, Viral