Protein kinases, nitric oxide and long-term depression of synapses in the cerebellum.
We have analysed the effects of polymyxin B, a potent inhibitor of calcium-dependent protein kinases, of L-N-monomethylarginine, an inhibitor of nitric oxide synthesis, and of methylene blue which prevents activation of soluble guanylate cyclase, on long-term depression of parallel fibre-mediated EPSPs of rat cerebellar Purkinje cells in slices maintained in-vitro. In control conditions, a long-term depression of parallel fibre-mediated EPSPs was consistently induced by their pairing with calcium spikes directly elicited in the postsynaptic cells. This long-term change in synaptic strength was not observed in the presence of polymyxin B, of L-N-monomethylarginine, or of methylene blue, suggesting that calcium-dependent protein kinases and nitric oxide are both involved.
Crepel
F
F
CNRS URA 1121, Laboratoire de Neurobiologie et Neuropharmacologie du Développement, Université Paris-Sud, Orsay, France.
Jaillard
D
D
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D005071:Evoked Potentials / Q000187:drug effects; D066298:In Vitro Techniques; D008751:Methylene Blue / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D011112:Polymyxin B / Q000494:pharmacology; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D013569:Synapses / Q000187:drug effects / Q000502:physiology*; D019323:omega-N-Methylarginine; D017871:Calcium-Calmodulin-Dependent Protein Kinases
D001120:Arginine; D008751:Methylene Blue; D009569:Nitric Oxide; D011112:Polymyxin B; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases; D019323:omega-N-Methylarginine; D017871:Calcium-Calmodulin-Dependent Protein Kinases