Characterization and use of monoclonal antibodies to identify Haemophilus paragallinarum serovars.
Two serovar-specific monoclonal antibodies (MAbs) to Haemophilus paragallinarum serovars A/1 and C/2 strains, respectively, were developed and characterized by hemagglutination-inhibition (HI) and dot-blotting tests using representative H. paragallinarum serovars A/1, B, and C/2 strains. In both the HI and dot-blotting tests, one MAb (E5C12D10), raised against strain 221, serovar A/1, reacted only with serovar A/1 strains, while the other MAb (F2E6), raised against strain S1 of serovar C/2, reacted with only serovar C/2 strains examined. In both tests, the two MAbs did not react with two serovar B strains. These results indicated that the two MAbs recognize serovar-specific hemagglutinating (HA) antigens of H. paragallinarum serovars A/1 and C/2 strains, respectively, and that a dot-blotting test using these MAbs is a practical alternative to the HI test for serotyping H. paragallinarum. Strains 0222 and Spross of serovar B, which did not react with these two MAbs, were found to possess serovar-specific HA antigen in cross-HI tests.
Yamaguchi
T
T
Aburahi Laboratories, Shionogi Research Laboratories, Shionogi & Co., Ltd., Shiga, Japan.
Takigami
S
S
Iritani
Y
Y
Hayashi
Y
Y
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D002645:Chickens; D005260:Female; D006190:Haemophilus / Q000145:classification* / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D051379:Mice; D012703:Serotyping; D013047:Specific Pathogen-Free Organisms
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D007106:Immune Sera