[Evaluation in our milieu of an ELISA method (ANDA-Tb) for the serological diagnosis of tuberculosis].
We have evaluated the new ELISA test (ANDA-Tb) for the serological diagnosis of tuberculosis in serum samples from 343 individuals: 160 were from blood donors, 152 from patients with nontuberculous diseases, most of them respiratory, and 31 from tuberculous patients diagnosed by positive culture. Using a cutoff value in 350 serounits we had a positive predictive value of 57% and a negative predictive value of 98% in blood donors, and 43% and 97%, respectively, in control patients. Sensitivity was 68%. There was a higher false positive rate in patients with respiratory diseases, particularly acute pneumonia. We conclude that this particular ELISA is very useful as a supportive test for the diagnosis of tuberculosis, with a lower specificity in hospital nontuberculous patients.
Pérez
J A
JA
Laboratorio de Microbiología, Hospital Ciudad de Coria.
Moreno
J J
JJ
Aguyo
A
A
Alvarez
M
M
Díez
P
P
Cuenca
C
C
Sanchidrián
I
I
Rodríguez
L
L
D000907:Antibodies, Bacterial / Q000032:analysis*; D001782:Blood Donors; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000276:immunology* / Q000302:isolation & purification; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D012120:Respiration Disorders / Q000276:immunology; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology
D000907:Antibodies, Bacterial; D011933:Reagent Kits, Diagnostic
Evaluación en nuestro medio de un método ELISA (ANDA-Tb) para diagnóstico serológico de tuberculosis.