Solution-phase synthesis of alpha-human atrial natriuretic peptide (alpha-hANP).
Alpha-human atrial natriuretic peptide (alpha-hANP) was synthesized by assembling six peptide fragments in solution followed by deprotection with HF and subsequent air-oxidation. The trimethylbenzyl group was employed as an S-protecting group of cysteine. The HF-dimethylselenide-m-cresol system was employed as a final deprotecting reagent and, at the same time, as a reducing reagent of Met(O). Synthetic alpha-hANP elicited potent diuretic and natriuretic activity in rats.
Yoshida
M
M
Department of Medicinal Chemistry, Kyoto Pharmaceutical University, Japan.
Shimokura
M
M
Fujiwara
Y
Y
Fujisaki
T
T
Akaji
K
K
Kiso
Y
Y
D000595:Amino Acid Sequence; D009320:Atrial Natriuretic Factor / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data
D009320:Atrial Natriuretic Factor