Polyclonal and monoclonal IgGs from MRL/Mp-lpr/lpr mice induce an interleukin-3-dependent cell line to produce interleukin-3 through an Fc gamma-receptor-mediated mechanism.
Serum IgG from autoimmune MRL/MP-lpr/lpr(MRL/l) mice induced IL-3 mRNA, IL-3 synthesis, and cell proliferation in the IL-3-dependent cell line, FDC-P2/185-4. Cell growth, induced by MRL/l-IgG, was suppressed by anti-IL-3 antibody, suggesting an autocrine mechanism. We established hybridomas from the splenocytes of a nonimmunized MRL/l mouse. The mAbs, produced by these hybridomas, also stimulated IL-3 synthesis and cell growth in FDC-P2/185-4 cells. Both anti-IgG-Fc antibody and anti-Fc gamma RII mAb, 2.4G2 suppressed the cell growth induced by these mAbs. Our results suggest that some IgGs of MRL/l mice may induce IL-3 synthesis in cells by binding to Fc receptors.
Sakihama
T
T
Department of Parasitology & Immunology, Yamanashi Medical College, Japan.
Akasu
F
F
Iwamoto
M
M
Shirakura-Shibata
Y
Y
Okada
Y
Y
Nakajima
Y
Y
Tasaka
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000943:Antigens, Differentiation / Q000502:physiology*; D001327:Autoimmune Diseases / Q000276:immunology*; D015152:Blotting, Northern; D002455:Cell Division; D002460:Cell Line; D007074:Immunoglobulin G / Q000276:immunology*; D007377:Interleukin-3 / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008817:Mice, Mutant Strains; D012333:RNA, Messenger / Q000235:genetics; D011961:Receptors, Fc / Q000502:physiology*; D017452:Receptors, IgG; D013329:Structure-Activity Relationship
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D007074:Immunoglobulin G; D007377:Interleukin-3; D012333:RNA, Messenger; D011961:Receptors, Fc; D017452:Receptors, IgG