Monoclonal antibody-coated latex agglutination assay for identification of Actinobacillus actinomycetemcomitans.
Three monoclonal antibodies (MAbs) to lipopolysaccharide of Actinobacillus actinomycetemcomitans strain Y4 (serotype b) and eight MAbs to a serotype b-specific polysaccharide antigen of strain Y4 were obtained. Latex particles sensitized with an MAb to the Y4 lipopolysaccharide produced a positive agglutination with whole cells of all three serotypes of A. actinomycetemcomitans, but not with Haemophilus aphrophilus, Haemophilus paraphrophilus, Haemophilus influenzae, Porphyromonas (Bacteroides) gingivalis, "Bacteroides" intermedius, Fusobacterium nucleatum and Escherichia coli. On the other hand, latex particles sensitized with an MAb to the serotype b-specific polysaccharide antigen agglutinated with whole cells of serotype b A. actinomycetemcomitans and P. gingivalis, but not with heated and trypsinized cells of P. gingivalis. The simple and rapid latex agglutination assay using MAbs may be useful for the identification of A. actinomycetemcomitans.
Koga
T
T
Department of Dental Research, National Institute of Health, Tokyo, Japan.
Senpuku
H
H
Nakashima
K
K
Ishihara
Y
Y
Nishihara
T
T
D000188:Actinobacillus / Q000276:immunology / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal; D006825:Hybridomas; D005779:Immunodiffusion; D007841:Latex Fixation Tests; D008070:Lipopolysaccharides / Q000276:immunology; D008863:Microspheres; D011135:Polysaccharides, Bacterial / Q000276:immunology
D000911:Antibodies, Monoclonal; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial