[The determination of the phenotypes of 3 enzyme systems by the polyacrylamide gel electrophoresis method].
Possibility of combined determination of phenotypes of three enzyme system (glyoxylase-1, phosphoglucomutase and 6-phosphogluconate dehydrogenase) in the apparatus with two vertical blocks of polyacrilamide gel (PAAG) was shown. The method is based on the principle of combination of differently composed blocks of PAAG and single universal electrode buffer. High productivity and economy of the method equally with good quality of resulting phoregrams make this method useful for medicolegal practical work.
Svirskiĭ
M S
MS
Kolmakova
M A
MA
D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods*; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D007791:Lactoylglutathione Lyase / Q000032:analysis*; D008190:Lyases / Q000032:analysis*; D010641:Phenotype; D010733:Phosphoglucomutase / Q000032:analysis*; D010734:Phosphogluconate Dehydrogenase / Q000032:analysis*
D010734:Phosphogluconate Dehydrogenase; D008190:Lyases; D007791:Lactoylglutathione Lyase; D010733:Phosphoglucomutase
Opredelenie fenotipov trekh fermentnykh sistem metodom élektroforeza v poliakrilamidnom gele.