Successful culturing of infectious, oncogenic adenovirus from the stimulated lymphocytes of a patient with bladder tumour.
During their attempts at culturing viruses from tumour cells and circulating lymphocytes, authors obtained oncogenic type 18 infectious adenovirus from the phytohaemagglutinin-stimulated, peripheral T lymphocytes of a patient with bladder tumour. They found that the lymphocytes of other patients with urogenital tumour often show sensibility to the viral antigens. The successful culturing of the virus proves that patients with urogenital tumour carry the functioning genomes of the ontogenic virus, not only in their tumour cells but also in their circulating lymphocytes. It is assumed that these genomes jointly with the immune system impaired by other DNS viruses might have a role in tumour development. It is known that the tumour cells and circulating lymphocytes of patients with malignant urogenital tumour may carry viral components, latent viruses. However, infectious virus--in the mentioned cases--could not be isolated. In the present study attempt was made to culture infectious virus from the tumour cells and the in vitro stimulated circulating T lymphocytes of patients with urogenital tumour.
Csata
S
S
Department of Urology, István Kórház, Budapest, Hungary.
Kulcsár
G
G
Nász
I
I
D000260:Adenoviruses, Human / Q000302:isolation & purification*; D000368:Aged; D002295:Carcinoma, Transitional Cell / Q000382:microbiology*; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000382:microbiology*; D001749:Urinary Bladder Neoplasms / Q000382:microbiology*; D014776:Virus Cultivation