Identification of Brucella abortus strain 19 by decreased ability to utilize erythritol as determined by gas liquid chromatography.
A method to identify Brucella abortus strain 19 by erythritol utilization using gas liquid chromatography (GLC) was developed. A total of 69 strains of B. abortus (41 virulent field strain isolates and 28 strain 19 isolates) were tested. Following incubation of the isolate with a standard amount of erythritol, the erythritol present in the cell suspension was acetylated and measured by GLC. Field strains of B. abortus utilized an average of 90.9% of the erythritol, whereas vaccine strains utilized an average of 42.4%. This difference in erythritol utilization will allow a more rapid identification of B. abortus strain 19.
Ewalt
D R
DR
United States Department of Agriculture, National Veterinary Services Laboratories, Ames, IA 50010.
Ross
P F
PF
Payeur
J B
JB
D000818:Animals; D015373:Bacterial Typing Techniques; D002003:Brucella abortus / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D002849:Chromatography, Gas; D004896:Erythritol / Q000032:analysis / Q000378:metabolism*
D004896:Erythritol