title: cDNA cloning and sequencing for the import precursor of subunit B in H(+)-ATP synthase from rat mitochondria.
abstract: The nucleotide sequence of the import precursor of subunit b of rat liver H(+)-ATP synthase has been determined from a recombinant cDNA clone isolated by screening a rat liver cDNA library with a probe DNA. The sequence was composed of 1,124 nucleotides including a coding region for the import precursor of subunit b and noncoding regions of both the 5'- and 3'-sides. The import precursor of subunit b and its mature polypeptide deduced from the open reading frame consisted of 256 and 214 amino acid residues with a molecular weight of 28,867 and 24,628, respectively. The presequence of 42 amino acids could be the import signal peptide which serves to direct the protein into the mitochondrial matrix.
authors: Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
authors: C
authors: C
authors: Tsurumi
authors: Y
authors: Y
authors: Yoshihara
authors: F
authors: F
authors: Osaka
authors: F
authors: F
authors: Yamada
authors: I
authors: I
authors: Tani
authors: T
authors: T
authors: Higuti
authors: M
authors: M
authors: Shimizu
authors: K
authors: K
authors: Oeda
authors: H
authors: H
authors: Ohkawa
authors: H
authors: H
authors: Toda
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D015723:Gene Library; D008930:Mitochondria, Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000032:analysis / Q000235:genetics*; D006180:Proton-Translocating ATPases / Q000032:analysis / Q000235:genetics*; D051381:Rats
chemical_list: D021382:Protein Sorting Signals; D004247:DNA; D006180:Proton-Translocating ATPases