Turnover of monoamine oxidase B (MAO-B) in pig brain by positron emission tomography using 11C-L-deprenyl.
Positron emission tomography (PET) was applied to investigate the rate of turnover of pig brain MAO-B. For this purpose 11C-L-deprenyl was used as an irreversible ligand, which bind stoichiometrically to the enzyme. A tracer dose of 11C-L-deprenyl was injected and PET scans performed to obtain baseline deprenyl binding. A high dose of unlabelled deprenyl was then administered to inhibit the enzyme and tracer doses of 11C-L-deprenyl, with subsequent PET analyses, were given at 0, 2, 7, 21 and 42 days. The half-life for the turnover rate calculated was found to be 6.5 days.
Oreland
L
L
Department of Medical Pharmacology, University Hospital, Uppsala, Sweden.
Jossan
S S
SS
Hartvig
P
P
Aquilonius
S M
SM
Långström
B
B
D000818:Animals; D001921:Brain / Q000033:anatomy & histology / Q000201:enzymology*; D002560:Cerebrovascular Circulation; D006207:Half-Life; D008995:Monoamine Oxidase / Q000378:metabolism*; D012642:Selegiline; D013552:Swine; D014055:Tomography, Emission-Computed
D012642:Selegiline; D008995:Monoamine Oxidase