Plasma aldosterone concentration during hypoxia, stimulated hyperglycaemia and when protein synthesis is blocked with actinomycin D.
In order to study the effect of various agents on aldosterone secretion, the aldosterone concentration was determined radioimmunologically in 41 rats divided in groups: group I--7 rats--controls; group II--11 rats loaded p.o. with 5.5% glucose solution in doses of 2.4 ml/100 g body weight, taking the blood sample 2 hours after the loading; group III--7 rats subjected to the same treatment, but the blood was taken 6 hours later; group IV--7 rats subjected to hypobaric hypoxy for 2 hours; group V--9 rats treated with 40 ug/100 g b. w. actinomycin D and then subjected to hypoxy for 2 hours. The blood sample from the last two groups was taken 3 hours after the end of the hypoxy. The radioimmunological determination was made using a kit of the firm Sorin--Biomedica. The results show that glucose and hypoxy act as stimulators of aldosterone secretion.
Ilieva
G
G
Department ol Physiology, Higher Medical Institute, Stara Zagora.
Tolekova
A
A
Logofetov
A
A
D000450:Aldosterone / Q000097:blood*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology*; D006943:Hyperglycemia / Q000097:blood*; D000860:Hypoxia / Q000097:blood*; D007202:Indicators and Reagents; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D001786:Blood Glucose; D007202:Indicators and Reagents; D003609:Dactinomycin; D000450:Aldosterone