Semiautomated preparation of DNA templates for large-scale sequencing projects.
The rate limiting step in a large-scale sequencing project is the generation of single-stranded DNA templates. We describe a fast, semiautomated procedure, using 96-well microtitre plates, in which 192 templates can be readily prepared in 1 day. The technique can be carried out manually or can be semiautomated using a robot pipetting device. We also provide evidence for the reliability and applicability of this method to a large-scale sequencing project.
Smith
V
V
Medical Research Council Laboratory of Molecular Biology, Cambridge.
Brown
C M
CM
Bankier
A T
AT
Barrell
B G
BG
D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D004279:DNA, Viral / Q000302:isolation & purification*; D004926:Escherichia coli / Q000235:genetics; D005821:Genetic Techniques; D012371:Robotics; D013698:Templates, Genetic
D004279:DNA, Viral