Growth inhibition caused by serum depletion induces differentiation, interleukin 1 receptor expression and interleukin 1 responsiveness in the HL-60 promyelocytic leukemia cell line.
The role of interleukin-1 (IL-1) was studied in the proliferation of promyelocytic HL-60 leukemia cells. When HL-60 cells were cultured in 10% fetal calf serum (FCS) containing medium IL-1 did not have any effect on the proliferation. In 1% FCS containing medium, the proliferation of HL-60 cells gradually decreased, but IL-1 was found to be clearly mitogenic for these cells. IL-1 did not function as an autocrine growth factor for HL-60 cells, since anti-IL-1 antibodies did not suppress the basal proliferation of these cells. IL-1 was also mitogenic for U937 but not for THP-1 cells. The suppression of HL-60 proliferation was found to be accompanied with monocytic differentiation as assessed by an increase in HLA-DR, CD11b and CD14 antigen expression. IL-1 could suppress this differentiation. HL-60 cells cultured in 1% FCS were found to express increased amounts of IL-1 receptors on the cell surface.
Silvennoinen
O
O
Department of Bacteriology and Immunology, University of Helsinki, Finland.
Hurme
M
M
D000943:Antigens, Differentiation / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects*; D002461:Cell Line, Transformed / Q000378:metabolism; D003470:Culture Media; D005312:Fetal Blood; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000494:pharmacology*; D011971:Receptors, Immunologic / Q000378:metabolism*; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins
D000943:Antigens, Differentiation; D003470:Culture Media; D007375:Interleukin-1; D015850:Interleukin-6; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins