A technique for microfilarial detection in preserved blood using nuclepore filters.
Nuclepore filtration is now the most widely used method of detecting microfilaremia, particularly if microfilariae are few in number. However, this system requires the blood sample to be processed promptly after collection. Using blood from Wuchereria bancrofti-infected patients, 3 solutions were tested to determine whether blood could be held for delayed processing. Of these, we identified 1, 2% formalin/10% Teepol, in which microfilaremic blood can be held for at least 9 mo without deterioration of microfilarial structure or decrease in microfilarial numbers. In addition, this mixture passed easily through a 5-microns filter at all times tested. Examination of more than 300 blood samples held in 2% formalin/10% Teepol showed that this solution can utilize the convenience and sensitivity of membrane filtration while eliminating the need to perform testing immediately after the blood is collected.
Dickerson
J W
JW
Parasitic Diseases Branch, Centers for Disease Control, U.S. Department of Health and Human Services, Atlanta, Georgia 30333.
Eberhard
M L
ML
Lammie
P J
PJ
D000818:Animals; D001793:Blood Preservation; D002951:Citrates; D019343:Citric Acid; D004492:Edetic Acid; D004605:Elephantiasis, Filarial / Q000097:blood* / Q000469:parasitology; D005233:Fatty Alcohols; D005374:Filtration; D005557:Formaldehyde; D006801:Humans; D008842:Microfilariae / Q000302:isolation & purification; D013501:Surface-Active Agents; D014958:Wuchereria bancrofti / Q000302:isolation & purification*
D002951:Citrates; D005233:Fatty Alcohols; D013501:Surface-Active Agents; D005557:Formaldehyde; D019343:Citric Acid; C003381:teepol; D004492:Edetic Acid