Endothelin induces thromboxane release and contraction of isolated guinea-pig airways.
The possible role of thromboxane (TX) A2 in mediating the bronchopulmonary effect of endothelin (ET) was studied in isolated guinea pig airways (trachea, upper bronchi and parenchyma). ET (1-100 nM) evoked a concentration-dependent contraction of all three tissues. The contractile response was significantly attenuated by pretreatment of the tissues with the specific thromboxane receptor blockers, BM 13177 (10-50 microM) and BM 13505 (1-5 microM). Furthermore, ET caused a dose-dependent TXB2 release from trachea and parenchyma. These findings suggest that the bronchopulmonary action of ET is mediated, in part, by the release of TXA2.
Filep
J G
JG
Department of Pharmacology, Faculty of Medicine, University of Sherbrooke, Quebec, Canada.
Battistini
B
B
Sirois
P
P
D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology; D001980:Bronchi / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D016232:Endothelins / Q000494:pharmacology*; D011464:Epoprostenol / Q000378:metabolism; D006168:Guinea Pigs; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects* / Q000502:physiology; D010648:Phenylacetates / Q000494:pharmacology; D011982:Receptors, Prostaglandin / Q000037:antagonists & inhibitors; D017482:Receptors, Thromboxane; D013449:Sulfonamides / Q000494:pharmacology; D013928:Thromboxane A2 / Q000378:metabolism*; D013929:Thromboxane B2 / Q000378:metabolism; D013931:Thromboxanes / Q000037:antagonists & inhibitors; D014132:Trachea / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*
D000889:Anti-Arrhythmia Agents; D016232:Endothelins; D010648:Phenylacetates; D011982:Receptors, Prostaglandin; D017482:Receptors, Thromboxane; D013449:Sulfonamides; D013931:Thromboxanes; D013929:Thromboxane B2; D013928:Thromboxane A2; C040624:sulotroban; D011464:Epoprostenol; C051762:daltroban