Endogenous prostaglandins: its role in gastric mucosal blood flow and ethanol ulceration in rats.
The involvement of endogenous prostaglandins (PGs) in modulating gastric mucosal blood flow (GMBF) is still unclear. The present study was designed to demonstrate the role of this autacoid in the basal GMBF and the restoration of blood flow after restriction of blood supply to the stomach. The ex-vivo gastric chamber was prepared and the GMBF was measured by a laser Doppler technique. 20% ethanol incubation for 10 min in the chamber increased the basal GMBF and lessened the reduction of blood flow induced by absolute ethanol. It also decreased lesion formation caused by ethanol. Indomethacin 5 mg/kg, given s.c 60 min before experimentation had the opposite effects. Ligation of the gastric artery for 20 min which reduced the GMBF by 60%, worsened ethanol ulceration. There was a marked rebound of the GMBF after the ligation was released. Indomethacin totally abolished the blood flow rebound and aggravated ethanol ulceration. However, 20% ethanol incubation significantly potentiated such a rebound in blood flow and reduced lesion formation. Indomethacin pretreatment reversed these actions, whereas misoprostol administration produced the similar effects as 20% ethanol. It is concluded that GMBF plays an important role in ethanol ulceration and both basal and rebound GMBF is probably modulated by endogenous PGs.
Cho
C H
CH
Department of Pharmacology, Faculty of Medicine, University of Hong Kong.
Chen
B W
BW
Hui
W M
WM
Luk
C T
CT
Lam
S K
SK
D000527:Alprostadil / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001783:Blood Flow Velocity / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology / Q000633:toxicity*; D005753:Gastric Mucosa / Q000098:blood supply*; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D016595:Misoprostol; D011453:Prostaglandins / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D013276:Stomach Ulcer / Q000139:chemically induced*
D011453:Prostaglandins; D016595:Misoprostol; D000431:Ethanol; D000527:Alprostadil; D007213:Indomethacin