title: Ultrastructural localization of calcium and Ca(2+)-ATPase activity in gonadotropes and stellate cells of the catfish pituitary.
abstract: In the pituitary of the African catfish, Clarias gariepinus, calcium precipitates were ultrastructurally visualized with the oxalate-pyroantimonate procedure (OPP). The presence of calcium in these precipitates was validated with several methods, including "Electron Energy Loss Spectrometry" (EELS). In the OPP-treated tissue calcium precipitates were seen in a) non-secretory stellate cells and b) gonadotropic (GTH-) cells. In the latter the amount of precipitate is generally low, but stimulation of the gonadotropin release, either in vivo or in vitro, resulted in a considerable increase. This increase is discussed in relation to the role of calcium as second messenger in the GTH-cells. Ca(2+)-ATPase was exclusively represented in stellate cells and GTH-cells, its strongest activity associated with the plasma membrane and with the membranes of the endoplasmic reticulum. The localization of this enzyme is discussed in relation to its role in the regulation of the intracellular calcium concentration in the GTH-cells. The stellate cells are considered to be involved in the regulation of extracellular calcium concentrations in the pituitary.
authors: Department of Experimental Zoology, Research Group for Comparative Endocrinology, Utrecht, The Netherlands.
authors: J
authors: J
authors: Peute
authors: A T
authors: AT
authors: van Linder
authors: M A
authors: MA
authors: Zandbergen
authors: W C
authors: WC
authors: de Bruijn
mesh_terms: D000818:Animals; D000965:Antimony; D002118:Calcium / Q000032:analysis*; D000252:Calcium-Transporting ATPases / Q000032:analysis*; D002397:Catfishes / Q000378:metabolism*; D005260:Female; D006065:Gonadotropins, Pituitary / Q000378:metabolism; D007150:Immunohistochemistry; D010070:Oxalates; D010868:Pimozide / Q000494:pharmacology; D010902:Pituitary Gland / Q000737:chemistry* / Q000166:cytology / Q000201:enzymology; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D006065:Gonadotropins, Pituitary; D010070:Oxalates; D010868:Pimozide; C009133:pyroantimonate; D000965:Antimony; D000252:Calcium-Transporting ATPases; D002118:Calcium