Localized cutaneous leishmaniasis (chiclero's ulcer) in Mexico: sensitivity and specificity of ELISA for IgG antibodies to Leishmania mexicana mexicana.
Sera from 223 human beings were assayed by enzyme-linked immunosorbent assay for immunoglobulin G antibodies to Leishmania mexicana mexicana promastigotes. 76% of 74 sera from patients with chiclero's ulcer gave positive reactions at a titre of 1:40; the positivity rate was lower in patients with long-standing infections. False positive reactions were obtained with only 7% of 56 sera from healthy individuals with positive Montenegro tests and with 6 of 7 sera from patients with Chagas disease. It is concluded that this method would be a useful contributory diagnostic method in cases of suspected chiclero's ulcer.
Garcia-Miss
M R
MR
Departamento de Immunología, Universidad Autonoma de Yucatan, México.
Andrade-Narvaez
F J
FJ
Esquivel-Viñas
R E
RE
Simmonds-Diaz
E B
EB
Canto-Lara
S B
SB
Cruz-Ruiz
A L
AL
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis; D000918:Antibody Specificity; D014355:Chagas Disease / Q000276:immunology; D002648:Child; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007894:Leishmania mexicana / Q000276:immunology*; D007896:Leishmaniasis / Q000276:immunology*; D008800:Mexico; D008875:Middle Aged; D012680:Sensitivity and Specificity
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G