title: Determination of the reducing terminal sugars of glycosaminoglycans using 2-aminopyridine.
abstract: The fluorescence labeling method (Takemoto, H. et al. (1985) Anal. Biochem. 145, 245-250) has been shown to have high sensitivity for measuring the sugar composition of glycoproteins. In the present study, its applicability for analysis of the reducing terminal sugars of glycosaminoglycans was investigated. The procedure involved coupling of glycosaminoglycans with 2-aminopyridine, followed by hydrolysis and N-acetylation, and then analysis by high-performance liquid chromatography on a reverse-phase column. The method was found to be useful for simultaneous determination of acidic, neutral and amino sugars at the reducing termini of glycosaminoglycan moieties.
authors: Department of Biochemistry, Hirosaki University School of Medicine, Japan.
authors: K
authors: K
authors: Takagaki
authors: T
authors: T
authors: Nakamura
authors: H
authors: H
authors: Kawasaki
authors: A
authors: A
authors: Kon
authors: S
authors: S
authors: Ohishi
authors: M
authors: M
authors: Endo
mesh_terms: D000631:Aminopyridines; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis*; D002809:Chondroitin Sulfates / Q000737:chemistry; D005456:Fluorescent Dyes; D006025:Glycosaminoglycans / Q000737:chemistry*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D014995:Xylosidases
chemical_list: D000631:Aminopyridines; D002241:Carbohydrates; D005456:Fluorescent Dyes; D006025:Glycosaminoglycans; D009844:Oligosaccharides; C062473:peptidochondroitin sulfate; D002809:Chondroitin Sulfates; D014995:Xylosidases; C056547:endo-beta-xylosidase; C032439:alpha-aminopyridine