In vitro autoantibody production by normal adult and cord blood B cells.
To investigate the repertoire of autoantibodies in humans, anti-DNA and rheumatoid factor (RF) production in vitro was assessed in cultures of adult peripheral blood B cells and neonatal umbilical venous blood B cells. B cells were stimulated under various culture conditions, using an immobilized monoclonal anti-CD3 antibody and adult T cells or Staphylococcus aureus (SA) in the presence or absence of adult T cells or factors derived from mitogen-stimulated adult T cells as polyclonal B cell activators. Total IgM, as well as IgM anti-DNA and RF, were assessed by ELISA. Total IgM production was induced from adult and neonatal B cells with SA plus T cell factors, as well as anti-CD3-stimulated T cells. RF was induced from adult and cord blood B cells by either mode of stimulation, whereas significant anti-DNA production was observed only when B cells were stimulated with anti-CD3-activated T cells. These results confirm the presence of B cell precursors for autoantibodies in the preimmune as well as normal adult repertoire, and indicate that the production of anti-DNA and RF appears to be regulated independently.
Pisetsky
D S
DS
Medical Service, Durham Veterans Administration Hospital, North Carolina 27705.
Jelinek
D F
DF
McAnally
L M
LM
Reich
C F
CF
Lipsky
P E
PE
D000328:Adult; D000974:Antibodies, Antinuclear / Q000096:biosynthesis*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D017252:CD3 Complex; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005312:Fetal Blood / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis; D011247:Pregnancy; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D012217:Rheumatoid Factor / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000276:immunology
D000974:Antibodies, Antinuclear; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D007075:Immunoglobulin M; D011948:Receptors, Antigen, T-Cell; D012217:Rheumatoid Factor