[Obtention of Histoplasma capsulatum exoantigens in neopeptone, glucose, thiamine and asparagine (NGTA) media].
The purpose of this work is obtaining exocellular antigens H and M from 4 H. capsulatum strains using NGTA medium (neopeptone, glucose, thiamine and asparagine) for periods of 1, 2 and 3 months, at 36 degrees C and continuously shaken. The exocellular antigens were evaluated by double immunodiffusion test against H. capsulatum rabbit antiserum, 7 histoplasmosis sera, 4 paracoccidioidomycosis sera and a reference antigen and antibody furnished by C.D.C. (Atlanta--USA). Except for the exocellular antigen from strain B.679 with 1 month of culture, all exocellular antigens obtained from the strains B.679, 58 and O187 showed the H and M bands. The A.811 strain demonstrated only the fraction H. All the exocellular antigens reacted positively with sera from histoplasmosis patients, except those obtained from strains 58 and B.679 with 1 month of culture. With regard to paracoccidioidomycosis patients sera, the exocellular antigens from strains 58 and O187 did not cross-react with them.
Garcia
N M
NM
Laboratório de Micologia Médica, Instituto de Medicina Tropical, São Paulo, Brasil.
de Assis
C M
CM
Del Negro
G M
GM
Lacaz
C da S
Cda S
D000946:Antigens, Fungal / Q000096:biosynthesis*; D003470:Culture Media; D006658:Histoplasma / Q000276:immunology*; D005779:Immunodiffusion / Q000379:methods
D000946:Antigens, Fungal; D003470:Culture Media
Obtenção de exoantígenos de Histoplasma capsulatum em meio de neopeptona, glicose, tiamina e asparagina (NGTA).