Limiting dilution analysis of alloreactive T-cell status and distribution during allograft rejection.
We have developed limiting dilution analysis techniques that discriminate between precursor T cells and T cells that have responded to prior contact with alloantigens. We used these techniques to monitor the behavior of functional T-lymphocyte subsets in sponge matrix allograft recipients. Thus, we were able to measure at the graft site and/or in the peripheral blood (1) all donor-reactive cytotoxic T lymphocytes, (2) the subset of donor-reactive cytotoxic T lymphocytes that has responded to graft alloantigens in vivo, (3) all donor-reactive helper T lymphocytes, and (4) the subset of donor-reactive helper T lymphocytes that has responded to graft alloantigens in vivo. In this communication, we describe some aspects of the in vivo T-cell response to allograft implantation that were revealed by this study.
Orosz
C G
CG
Ohio State University College of Medicine, Department of Surgery, Columbus 43210.
Bishop
D K
DK
D000818:Animals; D015704:CD4 Antigens / Q000276:immunology; D005260:Female; D006084:Graft Rejection / Q000276:immunology*; D007519:Isoantigens / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D013154:Spleen / Q000637:transplantation; D013601:T-Lymphocytes / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D014184:Transplantation, Homologous / Q000276:immunology
D015704:CD4 Antigens; D007519:Isoantigens