title: A specific member of the ATF transcription factor family can mediate transcription activation by the adenovirus E1a protein.
abstract: The adenovirus E1a protein stimulates transcription of viral early genes. Recent experiments indicate that E1a contains a transcriptional activating region, which functions when directed to a promoter. Because E1a is not a sequence-specific DNA binding protein, how it targets to viral promoters has been a question. Several of the viral early promoters contain one or more binding sites for ATFs, a family of cellular transcription factors. Here we show that E1a can function through a specific ATF protein, designated ATF-2. We provide evidence that E1a interacts with a discrete region of promoter-bound ATF-2, thereby positioning the E1a activating region at a viral promoter.
authors: Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
authors: F
authors: F
authors: Liu
authors: M R
authors: MR
authors: Green
mesh_terms: D051696:Activating Transcription Factors; D017456:Adenovirus Early Proteins; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D001798:Blood Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D006801:Humans; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D009363:Neoplasm Proteins / Q000378:metabolism; D015345:Oligonucleotide Probes; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D051696:Activating Transcription Factors; D017456:Adenovirus Early Proteins; D001798:Blood Proteins; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C052130:GAL4 protein, S cerevisiae; D009363:Neoplasm Proteins; D015345:Oligonucleotide Probes; D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors