Detection of anti-heart antibody in sera from patients with myocarditis or myocardiopathy by use of microcalorimetry.
Circulating autoantibodies have been commonly demonstrated in patients with viral heart disease. However, most of the previously established methods are limited to detection of circulating autoantibodies in sera, and the results correlate poorly with each other and with disease activity. Therefore, there is a need for new methods which can be used to study the resulting cellular effects after binding of autoantibodies to cell surface receptors and to correlate serum autoantibodies with disease activity. A microcalorimetric method was applied to detect circulating autoantibodies by measuring heat production of a line of cells from human embryo heart tissue. The instrument used was a thermoactive cell analyzer ESCO-3000 (Denshi kagaku Co, Musashino-shi, Tokyo). With the microcalorimeter, decreased heat production by cultured cells was demonstrated after admixture of anti-cellular antibody in vitro. Cardiac-specific autoantibodies were found in 53% of patients with viral heart disease. The microcalorimetric method of measuring anti-cellular antibody by monitoring heat production can be very useful and informative.
Shingu
M
M
Department of Virology, Kurume University School of Medicine, Japan.
Shingu
M
M
D000818:Animals; D000906:Antibodies / Q000032:analysis* / Q000502:physiology; D001790:Blood Physiological Phenomena; D001833:Body Temperature Regulation; D002151:Calorimetry / Q000379:methods*; D009202:Cardiomyopathies / Q000097:blood / Q000276:immunology*; D002478:Cells, Cultured; D003384:Coxsackievirus Infections / Q000473:pathology / Q000503:physiopathology; D006367:HeLa Cells / Q000502:physiology; D006801:Humans; D008297:Male; D051379:Mice; D009205:Myocarditis / Q000097:blood / Q000276:immunology*; D009206:Myocardium / Q000276:immunology* / Q000473:pathology
D000906:Antibodies