Morphological alterations of Xenopus oocytes induced by valine-14 p21rho depend on isoprenylation and are inhibited by Clostridium botulinum C3 ADP-ribosyltransferase.
Microinjection of the constitutively active recombinant Val-14 p21rho A into Xenopus oocytes induced dramatic morphological changes with redistribution of pigments from the animal pole resulting in spotted oocytes. The effects induced by Val-14 p21rho A were regulated by progesterone in a dose-dependent manner whereas prior ADP-ribosylation of the rho protein blocked its activity. About 30 min after microinjection, p21 rho was associated with the plasma membrane. The membrane association of p21rho and its biological activity were inhibited by lovastatin, an inhibitor of the 3-hydroxy-3-methylglutaryl coenzyme A reductase. The findings suggest that membrane attachment and biological activity of p21rho depend on isoprenylation of the GTP-binding protein.
Mohr
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Rudolph-Buchheim-Institut für Pharmakologie, Universität Giessen, FRG.
Just
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Hall
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Aktories
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D036002:ADP Ribose Transferases / Q000378:metabolism*; D000818:Animals; D001905:Botulinum Toxins; D002462:Cell Membrane / Q000378:metabolism; D003014:Clostridium botulinum / Q000201:enzymology; D003599:Cytoskeleton / Q000187:drug effects; D019204:GTP-Binding Proteins / Q000502:physiology*; D066298:In Vitro Techniques; D008148:Lovastatin / Q000494:pharmacology; D008565:Membrane Proteins / Q000494:pharmacology*; D008798:Mevalonic Acid / Q000378:metabolism; D008845:Microinjections; D009865:Oocytes / Q000187:drug effects*; D009866:Oogenesis / Q000187:drug effects*; D011106:Polyisoprenyl Phosphates / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology; D014982:Xenopus laevis; D020743:rhoB GTP-Binding Protein
D008565:Membrane Proteins; D011106:Polyisoprenyl Phosphates; D011374:Progesterone; D008148:Lovastatin; D036002:ADP Ribose Transferases; C063007:exoenzyme C3, Clostridium botulinum; D001905:Botulinum Toxins; D019204:GTP-Binding Proteins; D020743:rhoB GTP-Binding Protein; D008798:Mevalonic Acid