title: Relative positioning of the T cell and B cell determinants on an immunogenic peptide: its influence on antibody response.
abstract: A tryptic peptide of bovine beta-casein (amino acid residues 184-202) was used as a model antigen to investigate how the relative position of the (helper) T cell and B cell determinants on a protein antigen influences the antibody response. Immunization with the peptide elicited a considerably higher anti-peptide response in the C3H/He strain than in the C57BL/6 strain, despite the fact that the C57BL/6 T cells showed higher reactivity than the C3H/He T cells. The T cell and B cell determinants of the peptide were identified in these two strains. Each strain recognized a single B cell determinant and a single T cell determinant. In the C3H/He strain, the T and B cell determinants were located apart from one another, while the T and B cell determinants of the C57BL/6 strain were located in a region close each other. The results suggest that the level of an antibody response depends on the topological relationship of the T cell and B cell determinants on the antigen molecule.
authors: Department of Agricultural Chemistry, University of Tokyo, Japan.
authors: S
authors: S
authors: Hachimura
authors: A
authors: A
authors: Enomoto
authors: S
authors: S
authors: Kaminogawa
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D000941:Antigens; D001402:B-Lymphocytes / Q000276:immunology*; D002364:Caseins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008213:Lymphocyte Activation; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009842:Oligopeptides / Q000276:immunology; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
chemical_list: D000941:Antigens; D002364:Caseins; D009842:Oligopeptides