[T cell blastogenesis in stored blood can induce GVHD in open heart surgery].
We studied T cell blastogenesis in stored blood during the preservation time within 7 days. Blood samples were obtained from 8 adults and stored at 4 degrees C immediately after donation with CPD (citrate-phosphate-dextrose) for 3,5 and 7 days. After each intervals, mitogenic responses of T cells from the samples in the presence of an optimal dose of PHA as a mitogen and MLR (mixed lymphocyte reaction) with lymphocytes prepared by 2000 rad radiation from other 10 donors as stimulating cells were investigated. The stimulation index (S.I.) of PHA as a responsibility of the stored blood was decreasing to 86.7 +/- 24.8%, 44.2 +/- 12.5% and 31.2 +/- 11.1% in proportion of that of fresh blood after 3, 5 and 7 days preservation. The S.I. of MLR were in the same way decreasing to 92.5 +/- 21.8%, 45.9 +/- 9.8% and 33.3 +/- 10.1% after that. These observations suggest that lymphocytes in stored blood within 7 days are able to induce GVHD (graft-versus-host disease) in open heart surgery of which immunological activity is thought to be decreased by extra-corporeal circulation. We concluded that the stored blood within 7 days should be irradiated to 1500 rad or more in order to prevent GVHD.
Tajima
K
K
Department of Thoracic Surgery, Nagoya University School of Medicine, Japan.
Kawamura
M
M
Hibi
M
M
Okamoto
M
M
Itoh
S
S
Murakami
Y
Y
Abe
T
T
D000328:Adult; D001793:Blood Preservation; D006348:Cardiac Surgical Procedures; D006086:Graft vs Host Disease / Q000209:etiology*; D006801:Humans; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*
D010835:Phytohemagglutinins