title: [Evaluation of prekallikrein activator (PKA) activity in plasma-derived preparations].
abstract: The study was carried out on 111 series of human immunoglobulin preparations (81 series for intravenous use, 30 series for intramuscular use) produced abroad and in Poland. Kallikrein (KK) and prekallikrein activator (PKA) activities were identified using chromogenic substrate. S-2302 and proteinase inhibitors were tested by double immunodiffusion method. Several biochemical, biological and serological methods were also applied which are valid in the control of intravenous and intramuscular immunoglobulin preparations. It was found that trace amounts of PKA and KK are present in human immunoglobulin preparations for intravenous use such as Bioglobulina and Gamma-Venina (PKA 0.0092-0.0505 mu kat/L, and KK 0,-0.0093 mu kat/L). On the other hand normal human immunoglobulin preparations for intramuscular use had somewhat higher level of KK and PKA and anti-HBs Immunoglobulin preparations (5.50 mu kat/L and 153.91 mu kat/L) and Tetabulina preparations had very high levels of these factors as compared to remaining preparations (4.50 mu kat/L and 316.5 mu kat/L).
authors: Zakład Badania Surowic i Szczepionek PZH w Warszawie.
authors: B
authors: B
authors: Miks
authors: J
authors: J
authors: Aleksandrowicz
authors: J
authors: J
authors: Kawiak
mesh_terms: D005060:Europe; D015956:Factor XIIa / Q000032:analysis*; D006801:Humans; D007116:Immunization, Passive; D005779:Immunodiffusion / Q000379:methods; D007136:Immunoglobulins / Q000032:analysis*; D011044:Poland
chemical_list: D007136:Immunoglobulins; D015956:Factor XIIa
vernacular_title: Ocena aktywności aktywatora prekalikreiny (PKA) w preparatach osoczopochodnych.