title: A determination of the radial coordinate of Tyr-69 in F-actin using fluorescence energy transfer.
abstract: Fluorescence energy transfer was measured between Tyr-69 residues in an F-actin filament using 5-dimethylaminonaphthalene-1-sulfonyl chloride (DNS-Cl) as a fluorescence energy donor and 4-dimethylaminoazobenzene-4-sulfonyl chloride (DABSYL-Cl) as the acceptor. Both labels are covalently attached to Tyr-69 residues in an F-actin filament. Taking the helical structure of the F-actin filament into consideration, the radial coordinate of Tyr-69 was calculated to be in the range from 2.0 nm to 4.0 nm.
authors: Department of Anatomy, University of Sydney, N.S.W., Australia.
authors: M
authors: M
authors: Miki
authors: C G
authors: CG
authors: dos Remedios
mesh_terms: D000199:Actins / Q000737:chemistry*; D000818:Animals; D003619:Dansyl Compounds; D004735:Energy Transfer; D005453:Fluorescence; D007202:Indicators and Reagents; D011817:Rabbits; D013050:Spectrometry, Fluorescence; D014443:Tyrosine / Q000737:chemistry*; D004124:p-Dimethylaminoazobenzene / Q000031:analogs & derivatives
chemical_list: D000199:Actins; D003619:Dansyl Compounds; D007202:Indicators and Reagents; D014443:Tyrosine; C010572:4-N,N-dimethylaminoazobenzene-4'-sulfonyl chloride; D004124:p-Dimethylaminoazobenzene; C029038:dansyl chloride