title: Two alkaline phosphatase genes are expressed during early development in the mouse embryo.
abstract: Alkaline phosphatase (AP) activity is stage specific in mouse embryos and may be associated with compaction and separation of trophectoderm from inner cell mass in preimplantation development. We previously sequenced a cDNA and two mouse AP genes that could contribute to the AP activity in embryos. Oligonucleotide primers were constructed from the three sequences and used in the reverse transcription-polymerase chain reaction technique to establish that two of the three AP isozymes are transcribed during preimplantation development. The predominant transcript (E-AP) is from a gene highly homologous to the human tissue-specific APs, but different from the mouse intestinal AP. Tissue non-specific (TN) AP also is transcribed, but there is approximately 10 times less TN-AP than E-AP transcript. The TN-AP isozyme is the predominant transcript of 7 to 14 day embryos and primordial germ cells. A switch in predominance from E-AP to TN-AP must occur during early postimplantation development. This study establishes a framework for experiments to determine the functions of the two isozymes during preimplantation development.
authors: Department of Medical Biochemistry, University of Calgary Health Sciences Centre, Alberta, Canada.
authors: A C
authors: AC
authors: Hahnel
authors: D A
authors: DA
authors: Rappolee
authors: J L
authors: JL
authors: Millan
authors: T
authors: T
authors: Manes
authors: C A
authors: CA
authors: Ziomek
authors: N G
authors: NG
authors: Theodosiou
authors: Z
authors: Z
authors: Werb
authors: R A
authors: RA
authors: Pedersen
authors: G A
authors: GA
authors: Schultz
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D001755:Blastocyst / Q000201:enzymology; D004622:Embryo, Mammalian / Q000201:enzymology; D047108:Embryonic Development; D005260:Female; D005854:Germ Cells / Q000201:enzymology*; D007422:Intestines / Q000196:embryology* / Q000201:enzymology; D007527:Isoenzymes / Q000032:analysis / Q000235:genetics*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D013737:Testis / Q000201:enzymology
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D000469:Alkaline Phosphatase