Isolation and identification of human brain natriuretic peptides in cardiac atrium.
By using a radioimmunoassay (RIA) system newly established for human brain natriuretic peptide (BNP), a high concentration of immunoreactive (ir-) human BNP (hBNP) has been found in cardiac atrium (1). Two molecular forms of ir-hBNP of 4K and 13-15K were isolated from atrial extracts by using anti-hBNP IgG immunoaffinity chromatography and reverse phase high performance liquid chromatography (HPLC). By microsequencing, the peptides were determined to be a pro-hBNP (gamma-hBNP) and its C-terminal 32-amino acid peptide (hBNP-32). Based on these results, in cardiac atrium, hBNP is found to be processed in a pathway similar to that of porcine BNP (pBNP) but distinct from that of rat BNP, although low MW hBNP-32 is a major form in contrast to pBNP which exists as a high MW gamma-pBNP.
Hino
J
J
Department of Biochemistry, Miyazaki Medical College, Japan.
Tateyama
H
H
Minamino
N
N
Kangawa
K
K
Matsuo
H
H
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000032:analysis; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D006325:Heart Atria / Q000032:analysis; D006801:Humans; D008969:Molecular Sequence Data; D009206:Myocardium / Q000032:analysis*; D020097:Natriuretic Peptide, Brain; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D011863:Radioimmunoassay; D051381:Rats; D013552:Swine
D000596:Amino Acids; D009419:Nerve Tissue Proteins; D020097:Natriuretic Peptide, Brain; D009320:Atrial Natriuretic Factor