Temperature-dependent activation of Bacillus brevis metalloprotease expressed in mesophilic Bacillus subtilis.
When the Bacillus brevis secretory metalloprotease is expressed from the npr gene on a plasmid vector in the mesophile B. subtilis, grown at 37 degrees C, the enzyme was found to be properly processed, but secreted into the culture medium in a low-active conformation. Secreted metalloprotease can by heat-treatment (70 degrees C for 30 min) be converted into fully active enzyme.
Kostrov
S V
SV
Institute of Molecular Genetics, U.S.S.R. Academy of Science, Moscow.
Akimkina
T V
TV
Kaydalova
N V
NV
Strongin AYa
D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D003001:Cloning, Molecular; D004789:Enzyme Activation; D005798:Genes, Bacterial; D008666:Metalloendopeptidases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010957:Plasmids; D013696:Temperature
D008666:Metalloendopeptidases