title: [Determining free activity of cathepsin D in tissue homogenates after precipitation of cellular organelles in pH 5,0].
abstract: Homogenate is prepared in 0.25 M sucrose. Cellular organelles are precipitated by acidification to pH 5.0 and removed by centrifugation at 1500 x g during 30 minutes. In the obtained cytosol which does not contain lysosomes free activity of cathepsin D is determined.
authors: Zakładu Analizy Instrumentalnej Akademii Medycznej, Białymstoku.
authors: E
authors: E
authors: Skrzydlewska
authors: K
authors: K
authors: Worowski
authors: J
authors: J
authors: Jurkowski
authors: H
authors: H
authors: Ostrowska
mesh_terms: D000818:Animals; D002402:Cathepsin D / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D003470:Culture Media; D003600:Cytosol / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology* / Q000648:ultrastructure; D008954:Models, Biological
chemical_list: D003470:Culture Media; D002402:Cathepsin D
vernacular_title: Oznaczanie wolnej aktywności katepsyny D w homogenatach tkankowych po precypitacji organelli komórkowych w pH 5.0.