P1 gene expression in Drosophila larval fat body: induction by various ecdysteroids.
Using the developmental mutant ecd1, the biological activity of 20-hydroxyecdysone (20E) and 20E metabolites 3-dehydro-20-hydroxyecdysone (3D20E), 3-epi-20-hydroxyecdysone (3D20E), 3-epi-20-hydroxyecdysone-3-phosphate (20E'3P), 20,26-dihydroxyecdysone (20,26E), and 20-hydroxyecdysonoic acid (20Eoic) was tested for their ability to induce the transcription of the steroid-inducible gene P1 in the Drosophila larval fat body. 3D20E was the most efficient ecdysteroid in the initiation of P1 gene transcription therefore the formation of 3D20E and the 3-epimer could not be regarded as an inactivation pathway in Drosophila larvae. Formation of 20,26E and 20Eoic may be an inactivation pathway in this biological model.
Sommé-Martin
G
G
ENS, Departement de Biologie, CNRS URA 686, Laboratoire de l'Université Pierre et Marie Curie, Paris, France.
Colardeau
J
J
Beydon
P
P
Blais
C
C
Lepesant
J A
JA
Lafont
R
R
D000273:Adipose Tissue / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D015152:Blotting, Northern; D004330:Drosophila / Q000235:genetics* / Q000254:growth & development; D026461:Ecdysteroids; D015870:Gene Expression / Q000187:drug effects; D007447:Invertebrate Hormones / Q000494:pharmacology*; D007814:Larva; D008970:Molecular Weight; D009154:Mutation; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics*; D013696:Temperature
D026461:Ecdysteroids; D007447:Invertebrate Hormones; D012333:RNA, Messenger