Different promoter elements are required for the induced expression of c-fos and c-jun proto-oncogenes by the v-mos oncogene product.
The Mos protein is a serine/threonine protein kinase that is likely to be a part of signal transduction pathways that regulate cell growth. We show here that expression of the v-Mos protein leads to a transient transcriptional activation of the c-fos and the c-jun proto-oncogenes in NIH 3T3 cells. Different cis-acting promoter elements are responsible for this effect. In the c-fos promoter the dyad symmetry element (DSE, also known as serum response element, SRE) is sufficient to confer responsiveness to the v-Mos protein. In the c-jun promoter the 12-0-tetradecanoyl-phorbol-13-acetate (TPA) response element (TRE) mediates this effect. Various Mos mutants with decreased transforming activity have diminished trans-acting activity on the c-fos and c-jun promoters. These results suggest that the highly transforming v-Mos protein exerts at least some of its effects through the induction of expression of the c-fos and c-jun protooncogenes.
Schönthal
A
A
Department of Medicine, University of California, San Diego, La Jolla 92093.
Feramisco
J R
JR
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D017232:Oncogene Proteins v-mos; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011519:Proto-Oncogenes; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics; D014158:Transcription, Genetic
D017232:Oncogene Proteins v-mos; D015682:Retroviridae Proteins, Oncogenic; D015534:Trans-Activators; D004247:DNA