17 Beta-hydroxysteroid UDP-glucuronosyl transferase is expressed in bile ductular epithelial cells under physiological conditions.
Using an improved procedure to isolate pure bile ductular epithelial cells from rat liver, we were able to define UDP-glucuronosyl transferase (UDPGT) isozymes expressed in these cells under physiological conditions. The cells contained mRNA for a 17 beta-hydroxysteroid, a 3 alpha-hydroxysteroid and a phenol UDPGT. Concomitantly, a distinct pattern of UDPGT-immunoreactive proteins was expressed, including a putative 17 beta-hydroxysteroid UDPGT. The presence of this UDPGT isozyme was confirmed by a high level of testosterone glucuronidation activity. This is the first demonstration of a metabolic pathway in bile ductular epithelial cells that is primarily dedicated to the processing of endogenous compounds.
Franson
K L
KL
University of Zürich, Institute of Pharmacology and Biochemistry, Switzerland.
Burchell
B
B
Mathis
G A
GA
D000818:Animals; D001652:Bile Ducts / Q000166:cytology / Q000201:enzymology*; D004848:Epithelium / Q000201:enzymology; D014453:Glucuronosyltransferase / Q000096:biosynthesis*; D006914:Hydroxysteroids / Q000378:metabolism*; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D007668:Kidney / Q000201:enzymology; D010750:Phosphoproteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013739:Testosterone / Q000378:metabolism
D006914:Hydroxysteroids; D007527:Isoenzymes; D010750:Phosphoproteins; D012333:RNA, Messenger; D013739:Testosterone; D014453:Glucuronosyltransferase