Structure and nucleotide sequence of the Bacillus subtilis phenylalanyl-tRNA synthetase genes.
The nucleotide sequence of the Bacillus subtilis pheST genes coding for the 2 subunits of phenylalanyl-tRNA synthetase has been determined. The pheS gene corresponds to 1029 bp and the pheT gene to 2412 bp. The encoded proteins have Mrs of 38,947 (343 amino acids, alpha-subunit) and 87,916 (804 amino acids, beta-subunit), respectively. The genes are adjacent on the chromosome separated by only 15 nucleotides. The pheT gene is immediately followed by a hairpin structure typical of a rho-independent transcription terminator. S1 nuclease mapping and primer extension analysis of pheST mRNA revealed a major start of transcription 318 nucleotides upstream of the pheS gene, and 6 nucleotides downstream of a E sigma 43 promoter consensus sequence. Within the 5'-noncoding region several potential secondary structures have been noted.
Brakhage
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Institut für Mikrobiologie, Universität Münster, Germany.
Wozny
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Putzer
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D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D005809:Genes, Regulator / Q000235:genetics; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D010652:Phenylalanine-tRNA Ligase / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic / Q000235:genetics
D010652:Phenylalanine-tRNA Ligase