[Synthesis, cloning and expression of human alpha-atrial natriodiuretic peptide gene in yeast system].
Human alpha-ANP was synthesised using a yeast system. In order to obtain correct expression of the human alpha-ANP gene, a few bases on one strand were substituted by the corresponding yeast sequence, but those bases were not replaced in the complementary so a few unpaired bases existed. The gene was cloned into a shuttle vector (with alpha-factor). The recombinants containing different human alpha-ANP DNA sequences were separated by BglII restriction analysis. We compared the expression level of human alpha-ANP from two kinds of genes and the results showed that the ANP expression level by the gene containing yeast sequences was lower (0.5-0.7 mg/L) than that expressed by the original gene (0.8-1.0 mg/L).
Lin
H
H
Institute of Basic Medical Sciences, Beijing.
D009320:Atrial Natriuretic Factor / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005813:Genes, Synthetic; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D015003:Yeasts / Q000235:genetics*
D009320:Atrial Natriuretic Factor; D004247:DNA