title: Circulating antibodies against c-myc oncogene product in sera of colorectal cancer patients.
abstract: We developed a Western blot assay using purified human c-myc protein expressed in E. coli in order to look for circulating anti-c-myc antibodies in human sera. The presence of IgG antibodies against c-myc was observed in 25 out of 44 sera from patients with colorectal cancer diagnosed and treated at the Hôpital Curie in Paris, compared to the sera of 46 normal donors of which only 8 samples were positive (p = 0.001).
authors: Institut Jacques Monod, Université Paris VII, France.
authors: K
authors: K
authors: Ben-Mahrez
authors: I
authors: I
authors: Sorokine
authors: D
authors: D
authors: Thierry
authors: T
authors: T
authors: Kawasumi
authors: S
authors: S
authors: Ishii
authors: R
authors: R
authors: Salmon
authors: M
authors: M
authors: Kohiyama
mesh_terms: D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm / Q000097:blood*; D015153:Blotting, Western / Q000379:methods; D015179:Colorectal Neoplasms / Q000276:immunology*; D004247:DNA / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D015513:Oncogene Proteins / Q000276:immunology* / Q000302:isolation & purification; D009857:Oncogenes / Q000276:immunology*; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics
chemical_list: D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D007074:Immunoglobulin G; D015513:Oncogene Proteins; D011994:Recombinant Proteins; D004247:DNA