title: Application of in vivo electrochemistry to the measurement of changes in dopamine release during intracranial self-stimulation.
abstract: Stearate-modified graphite paste recording electrodes were acutely or chronically implanted into the nucleus accumbens along with bipolar stimulating electrodes in the ipsilateral ventral tegmental area (VTA). Chronoamperometry was used to monitor changes in electrochemical signals that may correspond to the oxidation of dopamine (DA) during experimenter-administered stimulation (EAS) and intracranial self-stimulation (ICS). Application of EAS to stimulating electrodes in the VTA produced increases in the electrochemical signal in both the anesthetized and conscious preparation. The magnitude of both effects increased as a function of current intensity. Initiation of ICS was also accompanied by an immediate increase in the electrochemical signal. Rate-intensity experiments revealed a corresponding increase in both the ICS rates and the electrochemical signal with successive increases or decreases in current intensity. In subsequent experiments, intraperitoneal injections of DA uptake blockers nomifensine and GBR-12909 produced significant increases in the amplitude of the chronoamperometric signal which corresponded to drug-induced increases in bar press rates. The noradrenergic uptake blocker desipramine had no significant effect on either ICS rates or oxidation current. These data indicate that ICS of the VTA may produce concurrent increases in DA neurotransmission in the nucleus accumbens. The pharmacological studies are consistent with a dopaminergic substrate of brain stimulation reward at electrode sites in the VTA.
authors: Department of Psychology, University of British Columbia, Vancouver, Canada.
authors: C D
authors: CD
authors: Blaha
authors: A G
authors: AG
authors: Phillips
mesh_terms: D000758:Anesthesia; D000818:Animals; D001921:Brain / Q000502:physiology*; D003891:Desipramine / Q000494:pharmacology; D004298:Dopamine / Q000737:chemistry / Q000378:metabolism*; D004563:Electrochemistry; D008297:Male; D014179:Neurotransmitter Uptake Inhibitors / Q000494:pharmacology; D009627:Nomifensine / Q000494:pharmacology; D010879:Piperazines / Q000494:pharmacology; D051381:Rats; D012201:Reward; D012653:Self Stimulation; D013681:Tegmentum Mesencephali / Q000502:physiology
chemical_list: D014179:Neurotransmitter Uptake Inhibitors; D010879:Piperazines; D009627:Nomifensine; C043425:vanoxerine; D003891:Desipramine; D004298:Dopamine