p53 interacts with p34cdc2 in mammalian cells: implications for cell cycle control and oncogenesis.
The p53 gene product has been implicated in both human and animal tumorigenesis. p53 complexes with the transforming proteins encoded by several different DNA tumour viruses. We demonstrate that human p53 is phosphorylated by the mammalian p34cdc2 kinase in vitro and coprecipitates with p34cdc2 in vivo. Our observations suggest that phosphorylation of p53 by p34cdc2 kinase may regulate the known activities of p53 in the initiation steps of DNA replication in mammalian cells.
Stürzbecher
H W
HW
Cell Proliferation Laboratory, Marie Curie Research Institute, Oxted, Surrey, UK.
Maimets
T
T
Chumakov
P
P
Brain
R
R
Addison
C
C
Simanis
V
V
Rudge
K
K
Philp
R
R
Grimaldi
M
M
Court
W
W
D000595:Amino Acid Sequence; D000818:Animals; D016203:CDC2 Protein Kinase; D002453:Cell Cycle / Q000502:physiology; D002460:Cell Line; D004261:DNA Replication / Q000502:physiology; D000882:Haplorhini; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D010766:Phosphorylation; D011233:Precipitin Tests; D011494:Protein Kinases / Q000494:pharmacology; D014162:Transfection; D016159:Tumor Suppressor Protein p53
D015513:Oncogene Proteins; D010750:Phosphoproteins; D016159:Tumor Suppressor Protein p53; D011494:Protein Kinases; D016203:CDC2 Protein Kinase