Restorative effects of thymosin fraction 5 on reduction in responsiveness of rat thymocytes to allogenic lymphocytes during bladder tumor induction.
One-way MLR was examined by using thymocytes (responder) from Fischer 344/NSIc rats in which urinary bladder cancers were induced by administration of N-butyl-N-(4-hydroxybutyl)nitrosamine [(BBN) CAS: 3817-11-6], and lymph node lymphocytes (stimulator) from WKH/Hkm rats. From the 10th week of the administration, when only hyperplastic change was noticed in the bladder mucosa, progressive decrease of the MLR was detected in the thymus small lymphocyte population (TSLP) and the decrease was the most conspicuous in more dense, namely, intermediate and heavier, small lymphocyte subpopulations. When TSLP was pretreated with TF5 in vitro, the most restorative effects on the decreased MLR were found in hyperplastic stage and the effects were becoming less with the advance of tumor developments. Moreover, in scrutinizing the effects of TF5 on the small lymphocyte subpopulation in hyperplastic stage, the maximal restoration of the responsiveness was found in the heavier subpopulation.
Asakawa
M
M
Department of Urology, Osaka City University Medical School, Japan.
Kinoshita
Y
Y
Kimura
S
S
Hato
F
F
Wada
S
S
Nishijima
T
T
Kishimoto
T
T
Maekawa
M
M
D000818:Animals; D002085:Butylhydroxybutylnitrosamine; D005260:Female; D007700:Kinetics; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D051381:Rats; D011916:Rats, Inbred F344; D011919:Rats, Inbred Strains; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013947:Thymosin / Q000494:pharmacology*; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology
D002085:Butylhydroxybutylnitrosamine; D013947:Thymosin