Applicability of the popliteal lymph node assay in the Brown-Norway rat.
A number of drugs and chemicals can induce autoimmune disorders. Previous studies suggested the popliteal lymph node (PLN) assay might be a suitable and reliable model to detect such compounds in the mouse. The applicability of this assay has been examined in the Brown-Norway rat using a panel of positive as well as negative reference compounds. Popliteal lymph nodes were excised and weighed seven days after subcutaneous injection of 5 mg of each compound into one footpad. Interestingly, all negative reference compounds produced no significant increase in PLN weight index whereas four positive reference compounds were associated with PLN-weight indices greater than 2.3. However, no increase in PLN weight was noted in procainamide- and isoniazide-treated rats as previously reported in the mouse. This first set of experiments indicates that the PLN assay is applicable to Brown-Norway rats and warrants further investigation.
Verdier
F
F
Laboratoire d'Immunotoxicologie Fondamentale et Clinique, INSERM U80-CNRS URA 1177-UCBL, Faculté de médecine A. Carrel, Lyon, France.
Virat
M
M
Descotes
J
J
D000818:Animals; D001327:Autoimmune Diseases / Q000139:chemically induced*; D004353:Drug Evaluation, Preclinical; D005260:Female; D007538:Isoniazid / Q000633:toxicity; D008198:Lymph Nodes / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D008297:Male; D009929:Organ Size / Q000187:drug effects; D011342:Procainamide / Q000633:toxicity; D051381:Rats; D011914:Rats, Inbred BN; D013311:Streptozocin / Q000633:toxicity
D013311:Streptozocin; D011342:Procainamide; D007538:Isoniazid