title: The effect of beta-endorphin on rat oocyte maturation in vitro.
abstract: Cumulus enclosed or denuded oocytes obtained from ovaries of 25- to 27-day Sprague-Dawley rats underwent spontaneous germinal vesicle breakdown (GVBD) when cultured for 6 h in Krebs-Ringer's buffered solution (KRBS). This spontaneous division was found to be inhibited by adding beta-endorphin to the culture system and the inhibition was dose dependent, ranging from 200 to 800 pg/ml KRBS. Naloxone, a potent opioid antagonist without any agonistic action, did not stimulate spontaneous GVBD when added to the KRBS at doses ranging from 80 to 120 pg/ml. However, by adding 80 pg/ml naloxone to the culture system containing 600 pg/ml beta-endorphin, the inhibitory effect of beta-endorphin on spontaneous GVBD could be reversed completely.
authors: Department of Anatomy, Faculty of Medicine, Hong Kong University.
authors: W S
authors: WS
authors: O
mesh_terms: D000818:Animals; D005260:Female; D066298:In Vitro Techniques; D008540:Meiosis / Q000187:drug effects; D009270:Naloxone / Q000494:pharmacology; D009865:Oocytes / Q000166:cytology / Q000187:drug effects*; D009866:Oogenesis / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D001615:beta-Endorphin / Q000494:pharmacology*
chemical_list: D009270:Naloxone; D001615:beta-Endorphin