[Comparison of several methods for detecting anti-erythrocyte alloantibodies].
The efficacy of different methods for anti-red cell antibodies detection was assessed, variations being found in accordance with the specificity of the alloantibodies. The usefulness of enzyme tests in anti-Rh antibody detection was demonstrated, as well as that of low ionic strength saline solutions in detecting anti-Kell, anti-Duffy and anti-Kidd antibodies. Serum precipitation with 15% polyethyleneglycol 8000 previously to indirect antiglobulin test was found the most sensitive method, providing the best results in all the antibodies studied.
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Instituto de Hematología e Inmunología, Ciudad de la Habana, Cuba.
D001789:Blood Group Antigens / Q000276:immunology*; D001787:Blood Group Incompatibility / Q000175:diagnosis*; D003298:Coombs Test / Q000379:methods*; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D006801:Humans; D007518:Isoantibodies / Q000032:analysis*; D008297:Male; D010206:Papain; D011092:Polyethylene Glycols; D011247:Pregnancy; D012203:Rh Isoimmunization / Q000175:diagnosis; D014357:Trypsin
D001789:Blood Group Antigens; D007518:Isoantibodies; D011092:Polyethylene Glycols; D014357:Trypsin; D010206:Papain
Comparación de varios métodos de detección de aloanticuerpos antieritrocitarios.