title: Two high affinity (Ca2+ + Mg2+)-ATPases of vascular smooth muscle plasma membrane preparation. Their relation to the Ca2(+)-pumping ATPase.
abstract: High affinity Ca2(+)-activated, Mg2(+)-dependent ATP-ase [Ca2+ + Mg2+)-ATPase) activities were characterized in the membrane fractions of the porcine aorta. The (Ca2+ + Mg2+)-ATPase activity, similar to those found in the plasma membranes of the erythrocyte and the heart, ie, Ca2(+)-pumping ATPase activity, was not found in the membrane fractions isolated by the conventional method. The activity, however, became apparent in a plasma membrane-enriched fraction obtained from the microsomes treated with digitonin. The enzyme activity was stimulated by a purified C-kinase and by cyclic GMP or a purified cyclic GMP-dependent protein kinase (G-kinase). In addition to the Ca2(+)-pumping ATPase which requires millimolar concentration of Mg2+ for its activity, another high affinity (Ca2+ + Mg2+)-ATPase was detected that required micromolar concentration of Mg2+ for its full activation. Cell fractionation studies suggested its localization to plasma membranes, but the biochemical characteristics of the enzyme indicated that the enzyme could not be a biochemical expression of the plasma membrane Ca2+ pump.
authors: Department of Pharmacology, Niigata University School of Medicine, Japan.
authors: Y
authors: Y
authors: Yoshida
authors: H T
authors: HT
authors: Sun
authors: S
authors: S
authors: Imai
mesh_terms: D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000302:isolation & purification / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000302:isolation & purification / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology; D004072:Digitonin; D066298:In Vitro Techniques; D008861:Microsomes / Q000201:enzymology; D009131:Muscle, Smooth, Vascular / Q000201:enzymology*; D011494:Protein Kinases / Q000494:pharmacology; D013552:Swine; D014638:Vanadates / Q000494:pharmacology
chemical_list: D014638:Vanadates; D011494:Protein Kinases; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases; D004072:Digitonin