A fibroblast heparan sulphate proteoglycan with a 70 kDa core protein is linked to membrane phosphatidylinositol.
Here we present evidence that a fibroblast heparan sulphate proteoglycan of approx. 300 kDa and with a core protein of apparent molecular mass 70 kDa is covalently linked to the plasma membrane via a linkage structure involving phosphatidylinositol. Phosphatidylinositol-specific phospholipase C releases such a heparan sulphate proteoglycan only from cells labelled with [35S]sulphate in the absence of serum. Cell cultures labelled with [3H]myo-inositol in the absence or presence of serum produce a radiolabelled heparan sulphate proteoglycan which was purified by gel-permeation chromatography and ion-exchange chromatography on MonoQ. Digestion with heparan sulphate lyase and analysis by gel-permeation chromatography and sodium dodecylsulphate-polyacrylamide gel-electrophoresis revealed that the 3H-label is associated with a core protein of apparent mass 70 kDa.
Schmidtchen
A
A
Department of Physiological Chemistry, University of Lund, Sweden.
Sundler
R
R
Fransson
L A
LA
D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D005347:Fibroblasts / Q000737:chemistry*; D006497:Heparan Sulfate / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D008563:Membrane Lipids / Q000737:chemistry*; D010716:Phosphatidylinositols / Q000737:chemistry* / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000196:embryology; D010738:Type C Phospholipases / Q000378:metabolism
D006497:Heparan Sulfate; D008563:Membrane Lipids; D010716:Phosphatidylinositols; D010738:Type C Phospholipases