Purification of bacteriophage DNA by gel filtration chromatography.
Two fast and effective methods for high-scale purification of linear phage lambda DNA and circular double-stranded M13 replicative form are presented. A substantial reduction of time is attained by avoiding the long-term CsCl gradient centrifugations and dialysis common to standard procedures. Biologically active DNA preparations, free of chromosomal DNA and RNA, are obtained by including a simple gel filtration chromatography as the last step of purification. Yields are comparable to those from previously described methods.
González
A
A
Departmento de Bioquímica e Bioloxía Molecular, Facultade de Bioloxía, Universidade de Santiago de Compostela, Spain.
Gómez-Márquez
J
J
D010582:Bacteriophage lambda / Q000235:genetics*; D002850:Chromatography, Gel / Q000379:methods*; D004270:DNA, Circular / Q000302:isolation & purification*; D004279:DNA, Viral / Q000302:isolation & purification*
D004270:DNA, Circular; D004279:DNA, Viral