[The assessment of the toxigenicity of clinical strains of Pseudomonas aeruginosa by an immunoenzyme method using nitrocellulose filters].
The enzyme immunoassay (EIA) on nitrocellulose filters was adapted for the detection of exotoxin A in 104 P. aeruginosa strains isolated from patients and the environment in surgical wards in hospitals of Moscow and Alma-Ata. The method was shown to be highly sensitive: it permitted the detection of 5.ng of P. aeruginosa exotoxin A. The screening of 104 P. aeruginosa clinical strains by means of EIA on nitrocellulose filters revealed that these strains exotoxin A in 88.5% of cases.
Brodinova
N S
NS
Aleksandrov
A D
AD
Kitarova
G G
GG
Moroz
A F
AF
D003101:Collodion; D004783:Environmental Microbiology; D005098:Exotoxins / Q000032:analysis*; D005374:Filtration / Q000295:instrumentation; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D007623:Kazakhstan; D009031:Moscow; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification / Q000472:pathogenicity*; D013503:Surgery Department, Hospital
D005098:Exotoxins; D003101:Collodion
Otsenka toksigennosti klinicheskikh shtammov Pseudomonas aeruginosa immunofermentnym metodom s ispol'zovaniem nitrotselliuloznykh fil'trov.