Simple and rapid method for purification of vinculin from bovine aorta.
A new rapid purification procedure has been developed for mammalian smooth muscle vinculin. Bovine aorta vinculin has been purified by a two-step procedure employing hydrophobic chromatography on phenyl-Sepharose and gel filtration. This procedure should be particularly useful for purifying vinculin from tissues in which it is present in a relatively low concentration. In addition, the new purification protocol provides highly pure vinculin free from active contaminants which reduce the low-shear viscosity of F-actin solution.
Kobayashi
R
R
Department of Biochemistry, Akita University, School of Medicine, Japan.
Tashima
Y
Y
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001011:Aorta / Q000737:chemistry*; D002417:Cattle; D002845:Chromatography / Q000379:methods*; D002850:Chromatography, Gel / Q000379:methods*; D003429:Cross Reactions / Q000276:immunology; D003598:Cytoskeletal Proteins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D010449:Peptide Mapping; D012685:Sepharose / Q000031:analogs & derivatives; D016596:Vinculin
D000596:Amino Acids; D003598:Cytoskeletal Proteins; D016596:Vinculin; C020927:phenyl-sepharose; D012685:Sepharose