Degradation of atrial natriuretic factor in mouse blood in vitro and in vivo: role of enkephalinase (EC 3.4.24.11).
The half-life of 125I-ANF (99-126) in mouse blood in vivo, evaluated after HPLC analysis, was approximately 0.5 min. This value was nearly doubled in mice pretreated with sinorphan, an enkephalinase inhibitor. 125I-ANF is also hydrolyzed by mouse blood in vitro but its half-life is much longer and only marginally modified by an enkephalinase inhibitor. Hence, although the peptidase is present in blood plasma and cells, its rate-limiting participation in the clearance of the hormone in vivo does not occur at the blood level.
Gros
C
C
Unité de Neurobiologie et Pharmacologie, (U. 109) de l'INSERM Centre Paul Broca, Paris, France.
Souque
A
A
Schwartz
J C
JC
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006207:Half-Life; D006868:Hydrolysis; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D008297:Male; D008657:Metabolic Clearance Rate / Q000187:drug effects; D051379:Mice; D015260:Neprilysin / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015244:Thiorphan / Q000031:analogs & derivatives / Q000494:pharmacology
D007457:Iodine Radioisotopes; C049331:racecadotril; D009320:Atrial Natriuretic Factor; D015244:Thiorphan; D015260:Neprilysin