Molecular cloning of rat Krev-1 cDNA and analysis of the mRNA levels in normal and NMU-induced mammary carcinomas.
Human Krev-1 has been reported to reverse the transformed phenotype in a variety of cell types containing activated ras. Most mammary carcinomas induced by N-nitrosomethylurea in the rat have been reported to exhibit activated ras. Here we report that rat Krev-1 has a very high degree of homology to human Krev-1. Furthermore, Krev-1 expression is high in both normal and neoplastic mammary glands. No difference was found in expression levels in tumors with and without ras activation. These data argue against a tumor suppressor function for Krev-1 in the in vivo rat mammary gland.
Hong
H J
HJ
Department of Human Oncology, University of Wisconsin-Madison 53792.
Hsu
L C
LC
Gould
M N
MN
D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D019204:GTP-Binding Proteins / Q000032:analysis*; D015723:Gene Library; D006801:Humans; D008325:Mammary Neoplasms, Experimental / Q000032:analysis* / Q000139:chemically induced; D008770:Methylnitrosourea; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis*; D012334:RNA, Neoplasm / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D011920:Rats, Inbred WF; D013047:Specific Pathogen-Free Organisms; D020668:rap GTP-Binding Proteins
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D008770:Methylnitrosourea; D019204:GTP-Binding Proteins; D020668:rap GTP-Binding Proteins