Brain receptor autoradiography with [3H]-YM 09151-2: a ligand for labeling dopamine D-2 receptors.
Using the technique of in vitro receptor autoradiography to slide-mounted tissue sections, we studied the suitability of [3H]-YM-09151-2 as a ligand for labeling D-2 receptors in adult F344 rat brains. Specific [3H]-YM-09151-2 binding accounted for 70-80% of the total bound ligand and reached equilibrium after a 60-90 minute incubation. Scatchard analysis revealed a Kd of 626 pM. The apparent Bmax was 23.2 fmol/tissue section. Autoradiographs demonstrated high grain densities in the striatum and olfactory tubercle. Diffuse specific binding was also observed in the cortex.
Unis
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Department of Psychiatry and Behavioral Sciences, University of Washington, Seattle 98195.
Vincent
J G
JG
Dillon
B
B
D000818:Animals; D001345:Autoradiography; D001549:Benzamides / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D002421:Caudate Nucleus / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D007700:Kinetics; D009830:Olfactory Bulb / Q000378:metabolism; D011699:Putamen / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D011954:Receptors, Dopamine / Q000378:metabolism*; D017448:Receptors, Dopamine D2
D001549:Benzamides; D011954:Receptors, Dopamine; D017448:Receptors, Dopamine D2; C030265:nemonapride