[Effect of aging on interleukin 3 and its mRNA levels expression in mouse lymphocytes].
In this study, we determined IL-3 and its mRNA levels in T cells from C57BL/6J mice of different age groups (5, 12, 23 and 37 months). Spleen lymphocytes were stimulated with ConA and IL-3 activity was assayed by their ability to support the growth of an IL-3 dependent cell line, FDCP-1. IL-3 mRNA was determined by cytoplasmic dot-blot hybridization. mRNA reached maximum levels after 20 h of incubation with ConA in all 4 groups. The IL-3 activity and mRNA level in the 37 month age group showed 79.3% and 74% decreases respectively in comparison with the 5 month age group. The IL-2 and IL-3 mRNA levels also showed a parallel decline with age.
Li
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Institute of Medicinal Biotechnology, Beijing.
D000375:Aging / Q000502:physiology*; D000818:Animals; D015152:Blotting, Northern; D007377:Interleukin-3 / Q000097:blood*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000097:blood*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*
D007377:Interleukin-3; D012333:RNA, Messenger