[Human LAK cell induction and its biological characteristics].
LAK cell activity of peripheral blood from normal donors was induced by interleukin 2 (IL-2). Optimal induction of LAK cells was obtained by 3-4 day incubation of PBMC in the presence of 30-40 units/ml of IL-2. Several cell lines Raji, CEM, HeLa, 109 (esophageal carcinoma) and OC (ovarian carcinoma) which were NK cell resistant tumor cells could be lysed by LAK cells. PHA was unable to markedly induce the LAK cell activity. Although both LAK and NK cell activity was decreased by mitomycin C treatment, their degree of susceptibility to such treatment was quite different. NK cells were more sensitive to mitomycin C than LAK cells. In contrast to the significant inhibition of the NK cell-mediated lysis by the addition of anti-CD 2 monoclonal antibody during cytotoxicity assay LAK cell-mediated lysis was not affected. These results indicate that LAK cells are capable of lysing a variety of tumor cells that are resistant to NK cells and their biological characteristics are also different.
Wu
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Cancer Institute, Chinese Academy of Medical Sciences, Beijing.
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015703:Antigens, CD / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D004938:Esophageal Neoplasms / Q000473:pathology; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D010051:Ovarian Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000276:immunology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D007376:Interleukin-2; D008937:Mitomycins; D016685:Mitomycin