title: Brain natriuretic peptide is cosecreted with atrial natriuretic peptide from porcine cardiocytes.
abstract: Using primary cultures of atrial cardiocytes from neonatal pig, the secretion brain natriuretic peptide (BNP) and atrial natriuretic peptide (ANP)-like immunoreactivities (LI) was studied in vitro. Porcine cardiocytes time-dependently secreted both BNP-LI and ANP-LI into medium under a serum-free condition, although the amount of BNP-LI secreted was about one-third that of ANP-LI. Phorbol ester and Ca2+ ionophore had less stimulatory effects on secretion of BNP-LI than that of ANP-LI. Reverse-phase HPLC of the conditioned medium revealed a single major BNP-LI component corresponding to synthetic porcine BNP(1-26). These data suggest that a small molecular weight form BNP, possibly BNP(1-26), is cosecreted with ANP from porcine cardiocytes.
authors: Department of Biochemistry, Nihon University School of Medicine, Tokyo, Japan.
authors: T
authors: T
authors: Iida
authors: Y
authors: Y
authors: Hirata
authors: N
authors: N
authors: Takemura
authors: K
authors: K
authors: Togashi
authors: S
authors: S
authors: Nakagawa
authors: F
authors: F
authors: Marumo
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000302:isolation & purification* / Q000378:metabolism; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000032:analysis; D009206:Myocardium / Q000032:analysis / Q000378:metabolism*; D020097:Natriuretic Peptide, Brain; D009419:Nerve Tissue Proteins / Q000302:isolation & purification* / Q000378:metabolism; D011863:Radioimmunoassay; D013552:Swine
chemical_list: D003470:Culture Media; D009419:Nerve Tissue Proteins; D020097:Natriuretic Peptide, Brain; D009320:Atrial Natriuretic Factor