Very high frequency of reversion to guanidine resistance in clonal pools of guanidine-dependent type 1 poliovirus.
We have carefully examined the frequency of guanidine-resistant revertants in six different clonal pools of guanidine-dependent mutants of type 1 poliovirus. The mutation frequency was (6.5 +/- 6.3) x 10(-4) (with all amino acid substitutions occurring at position 227). The minimal corrected base substitution frequency per single nucleotide site in the codon for amino acid 227 was (2.1 +/- 1.9) x 10(-4).
de la Torre
J C
JC
Institute of Molecular Genetics, University of California, San Diego, La Jolla 92093.
Wimmer
E
E
Holland
J J
JJ
D001483:Base Sequence; D003062:Codon / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D019791:Guanidine; D006146:Guanidines / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D010641:Phenotype; D017955:Poliovirus / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D010948:Viral Plaque Assay
D003062:Codon; D006146:Guanidines; D015345:Oligonucleotide Probes; D019791:Guanidine