[Evaluation of a molecular hybridization trial for the detection of circulating DNA of hepatitis B virus. Immunoclinical correlation].
Immunoclinical correlation of the HBV circulating DNA was performed. The DNA was detected by means of a hybridization technique set-up in fluid phase using a complementary DNA probe labelled with 125I (Abbott-Genostics). The correlation was carried-out in 12 serum including 8 from known HBV carriers with or without liver disease. Only the positive HBeAg serum shown circulating viral DNA in ranges between 60 to 2800 pcg/ml. The assay shows to be easy to perform, however the expensive cost plus the use of a short-life isotope makes difficult in routine use, which still is under research. The immunopathogenic significance of the findings is discussed.
Zabaleta
M
M
Seccion de Inmunología Gastrointestinal, Centro Nacional de Referencia en Inmunología Clínica, Caracas.
Toro
F
F
Mariño
V
V
García
R
R
Machado
I V
IV
D004279:DNA, Viral / Q000032:analysis*; D006515:Hepatitis B virus / Q000235:genetics*; D006525:Hepatitis, Viral, Human / Q000276:immunology; D009693:Nucleic Acid Hybridization
D004279:DNA, Viral
Evaluación de un ensayo de hibridización molecular para la detección de ADN circulante del virus de hepatitis B: correlación inmunoclínica.