title: [Reconstruction of purified Ca2+,Mg2+-ATPase from the myometrium sarcolemma into liposomes and its catalytic properties].
abstract: The Ca2+, Mg2(+)-ATPase of the myometrium sarcolemma purified by the method of affinity chromatography on calmodulin sepharose is reconstituted into azolectin liposomes in the functionally active form by means of cholate dialysis. The ATPase-dependent accumulation of 45Ca is shown on the obtained model system. It makes up 95% of the total accumulation and may decrease to 43% under the effect of 0.8 microM A23187. Ca2+, Mg2(+)-ATPase reconstituted into azolectin liposomes is in the high affinity to Ca2+; Km for Ca2+ is equal to 0.88 +/- 0.22 microM, calmodulin practically does not change it. The highest activity of the reconstituted enzyme is observed at pH 7.0, temperature 50 degrees C, the Mg-ATP concentration 1-2 mM. The Km for substrate is 0.45 +/- 0.02 mM.
authors: M D
authors: MD
authors: Kurskiĭ
authors: N N
authors: NN
authors: Slinchenko
authors: L A
authors: LA
authors: Liubakovskaia
mesh_terms: D000818:Animals; D001692:Biological Transport; D017301:Ca(2+) Mg(2+)-ATPase / Q000302:isolation & purification / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000302:isolation & purification / Q000378:metabolism*; D002384:Catalysis; D005260:Female; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008081:Liposomes; D009215:Myometrium / Q000201:enzymology*; D012508:Sarcolemma / Q000201:enzymology*; D013552:Swine
chemical_list: D008081:Liposomes; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases; D002118:Calcium
vernacular_title: Rekonstruktsiia ochishchennoĭ Ca2+,Mg2+-ATP-azy sarkolemmy miometriia liposomy i ee kataliticheskie svoĭstva.