[Fast lipoprotein chromatography: a new method for determining plasma lipoproteins].
Fast Lipoprotein Chromatography (FLPC) is a novel method for the quantitation of lipoprotein fractions. Plasma proteins are separated by fast flow gel filtration and lipoproteins are detected after post-column derivatization with an enzymatic cholesterol reagent. FLPC resolves VLDL, LDL, and HDL. Coefficients of variation for determinations of VLDL-, LDL-, and HDL-cholesterol are 5.8%, 2.0%, and 1.9%, respectively. Comparison of FLPC with a combined ultracentrifugation and precipitation method obtained correlation coefficients of 0.983, 0.973, and 0.941 for VLDL-C, LDL-C, and HDL-C, respectively.
März
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Gustav-Embden-Zentrum der Biologischen Chemie, J.W. Goethe-Universität, Frankfurt am Main.
Siekmeier
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R
Scharnagl
H
H
Gross
W
W
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000097:blood; D001055:Apolipoproteins B / Q000097:blood; D002784:Cholesterol / Q000097:blood; D002845:Chromatography / Q000379:methods*; D006801:Humans; D008074:Lipoproteins / Q000097:blood*
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D001055:Apolipoproteins B; D008074:Lipoproteins; D002784:Cholesterol
Fast Lipoprotein Chromatography (FLPC): Eine neue Methode zur Bestimmung von Plasmalipoproteinen.