The HL-60 model for the interaction of human macrophages with the Legionnaires' disease bacterium.
The facultative intracellular pathogen, Legionella pneumophila, multiplies within and kills human monocytes and alveolar macrophages. We show that L. pneumophila strain Philadelphia-1 infects, multiplies within and kills the promyelocyte HL-60 cell line after its differentiation into macrophage-like cells. The characteristics of the interaction between L. pneumophila and differentiated HL-60 cells closely resemble those between L. pneumophila and human peripheral blood monocytes. With both cell types, C receptors and serum C mediate attachment of L. pneumophila, which are taken up by coiling phagocytosis. The replicative phagosome is lined with ribosomes; intracellular multiplication is iron-dependent; and replicating bacteria ultimately destroy the host cell. As in human monocytes, an avirulent mutant derivative of L. pneumophila Philadelphia-1, 25D, does not replicate in and is not cytopathic for differentiated HL-60 cells. Differentiated HL-60 cells therefore provide a convenient and faithful model for the study of L. pneumophila-mononuclear phagocyte interaction.
Marra
A
A
Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
Horwitz
M A
MA
Shuman
H A
HA
D001422:Bacterial Adhesion; D002448:Cell Adhesion; D003176:Complement C3 / Q000378:metabolism; D003165:Complement System Proteins / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D007501:Iron / Q000502:physiology; D007875:Legionella / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity; D008264:Macrophages / Q000276:immunology / Q000382:microbiology*; D010587:Phagocytosis; D010588:Phagosomes / Q000648:ultrastructure; D011951:Receptors, Complement / Q000502:physiology; D017463:Receptors, Complement 3b; D012270:Ribosomes / Q000648:ultrastructure; D014407:Tumor Cells, Cultured
D003176:Complement C3; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D003165:Complement System Proteins; D007501:Iron