title: Efficacy of immunopriming prior to isolation of tumor infiltrating lymphocytes for use in adoptive immunotherapy.
abstract: Adoptive immunotherapy is dependent upon the leukocytic subsets isolated as tumor infiltrating lymphocytes (TIL) prior to in vitro expansion with interleukin-2. To favorably influence T-cell subset representation in TIL the efficacy of bacillus Calmette Guerin (BCG) and cyclophosphamide (CTX) priming was evaluated in rats bearing Dunning R3327-AT prostatic tumors. When assessed by immunohistochemistry, both agents significantly (p less than 0.001) increased helper-T representation and decreased that by suppressor-T cells. As a result helper/suppressor (H/S) T cell ratios of TIL from untreated tumors (0.73 +/- 0.11) were significantly (p less than 0.001) elevated by both BCG (1.93 +/- 0.39) and CTX (1.40 +/- 0.25). Immunopriming might enhance adoptive immunotherapy by increasing the H/S ratio of TIL prior to their culture.
authors: Department of Research Biochemistry, Hektoen Institute for Medical Research, Chicago, Illinois 60612.
authors: M
authors: M
authors: Rubenstein
authors: S
authors: S
authors: Muchnik
authors: M
authors: M
authors: Chet
authors: M W
authors: MW
authors: Shaw
authors: A
authors: A
authors: Dubin
authors: P
authors: P
authors: Guinan
mesh_terms: D000818:Animals; D002469:Cell Separation; D003520:Cyclophosphamide / Q000494:pharmacology; D016219:Immunotherapy, Adoptive; D016246:Lymphocytes, Tumor-Infiltrating / Q000187:drug effects / Q000276:immunology*; D008297:Male; D009163:Mycobacterium bovis / Q000276:immunology; D011471:Prostatic Neoplasms / Q000276:immunology / Q000628:therapy; D051381:Rats; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology
chemical_list: D003520:Cyclophosphamide