Shuttle mutagenesis of Neisseria gonorrhoeae: pilin null mutations lower DNA transformation competence.
The method of shuttle mutagenesis has been extended to Neisseria gonorrhoeae. We have constructed a defective mini-Tn3 derivative that encodes chloramphenicol resistance in both N. gonorrhoeae and Escherichia coli and selected for mutations in the chloramphenicol resistance gene that express higher levels of antibiotic resistance in N. gonorrhoeae. Isogenic N. gonorrhoeae strains that differ only in pilin expression were constructed and used to test the effect of pilin null mutations on DNA transformation competence.
Seifert
H S
HS
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Ajioka
R S
RS
Paruchuri
D
D
Heffron
F
F
So
M
M
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics*; D004352:Drug Resistance, Microbial; D036781:Fimbriae Proteins; D009154:Mutation; D009344:Neisseria gonorrhoeae / Q000235:genetics*; D014170:Transformation, Genetic
D001425:Bacterial Outer Membrane Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D036781:Fimbriae Proteins; D015500:Chloramphenicol O-Acetyltransferase