Differential response of cadmium toxicity towards Mg2(+)-ATPase activity in hepatic and renal nuclear membrane in rats.
Treatment with cadmium chloride (40mg/kg body wt/day) for three days led to a marked inhibition of Mg2(+)-ATPase activity in rat liver nuclear membrane, whereas it stimulated the enzyme in renal nuclear membrane. On 30 days treatment (15 mg/kg body wt/day) the effect was totally different i.e. stimulation of enzyme activity in the liver and inhibition in the kidney tissue. Arrhenius plot analysis of the enzyme activity showed significant increase in phase transition temperature only in liver tissue of rats subjected to acute treatment. Lineweaver Burk plots also showed differential effect of cadmium toxicity on the enzyme activity, i.e. while both Km and Vmax were changed in the liver, there was change only in Km of enzyme from kidney.
Das
M
M
Department of Biochemistry, University College of Science, Calcutta, India.
Chatterjee
G C
GC
D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D002104:Cadmium / Q000633:toxicity*; D019256:Cadmium Chloride; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D009685:Nuclear Envelope / Q000201:enzymology; D009928:Organ Specificity; D051381:Rats
D002104:Cadmium; D017301:Ca(2+) Mg(2+)-ATPase; D019256:Cadmium Chloride