Production and characterization of monoclonal antibodies against tissue-type plasminogen activator.
Four hybridoma clones, TA1, TA2, TA3 and TA4, producing monoclonal antibodies against t-PA were obtained by fusion of mouse myeloma cells (SP2/0 or NS-1) with mouse spleen cells previously immunized with purified t-PA. The antibody titers of the four hybridoma ascites were higher than 1 x 10(5) determined by ELISA. The double immune diffusion test showed that all hybridoma supernatants contained mouse IgG1. These monoclonal antibodies reacted only with t-PA, and rt-PA prepared by genetic engineering, but not with UK. t-PA, activity was inhibited by these monoclonal antibodies completely.
Ding
H
H
Department of Molecular Genetics, Shanghai Medical University.
Wang
J Y
JY
Song
H Y
HY
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010959:Tissue Plasminogen Activator / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D010959:Tissue Plasminogen Activator