title: Serological survey for two simian retroviruses in macaques and African green monkeys.
abstract: Colonies of nonhuman primates at the Bowman Gray School of Medicine (BGSM) were tested for antibodies to two retroviruses associated with immunodeficiency by indirect immunofluorescence (IFA) and western blot. A total of 471 cynomolgus macaques (Macaca fascicularis), 144 rhesus monkeys (M. mulatta) and 67 stumptail monkey M. arctoides) were tested for SRV-1, and 152 African green monkeys (Cercopithecus aethiops) were tested for SIV. Of the macaques tested, 170 (36%) cynomolgus, 5 (3%) rhesus and 8 (12%) stumptails were positive for SRV-1 antibodies by IFA. Of the African green monkeys, 54 (36%) were IFA positive for SIV antibodies. A total of 143 African green monkeys tested by IFA also were tested by western blot. In the African green monkeys, the IFA had a positive predictive value of 98% and a negative predictive value of 96%. Of 176 IFA positive macaque sera tested by western blot, 49 (28%) were positive, 55 (31%) were considered equivocal (only one band, usually to p27 core protein), and 72 (41%) were negative.
authors: Department of Comparative Medicine, Wake Forest University Medical Center, Winston-Salem, NC 27103.
authors: L
authors: L
authors: Krugner-Higby
authors: L
authors: L
authors: Kucera
authors: N
authors: N
authors: Lerche
authors: J
authors: J
authors: Sever
authors: W
authors: W
authors: Fucillo
authors: J
authors: J
authors: Allan
authors: R
authors: R
authors: Benveniste
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D015153:Blotting, Western; D002522:Chlorocebus aethiops / Q000097:blood / Q000276:immunology; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D008251:Macaca / Q000097:blood / Q000276:immunology*; D008252:Macaca fascicularis / Q000097:blood / Q000276:immunology; D008253:Macaca mulatta / Q000097:blood / Q000276:immunology; D015302:Simian Immunodeficiency Virus / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral