Glycolipid- and glycoprotein-based blood group A antigen expression in human thrombocytes. A1/A2 difference.
Total non-acid glycolipid fractions and total sodium dodecylsulphate (SDS) solubilized protein fractions were isolated from human thrombocytes obtained from single human donors having different blood group A1/A2 phenotypes. The blood group A glycolipid antigens were characterized by immunostaining of thin layer plates with different monoclonal anti-A antibodies. The glycoproteins carrying blood group A epitopes were identified by SDS-PAGE and Western blot analysis using a monoclonal anti-A antibody. Blood group A glycolipid antigens were found in both A1 and A2 thrombocytes but the A2 individuals expressed at least ten times less A glycolipids compared to the A1 individuals. Expression of A type 3/4 chain and small amounts of A type 1 chain glycolipids were seen in thrombocytes of both A1 and A2 individuals, while the type 2 chain A glycolipids appeared to be missing from the A2 thrombocytes. Blood group A reactive glycoproteins were only found in thrombocytes of A1 individuals and could not be detected in A2 individuals or a blood group O individual. The major blood group A glycoprotein were found as a double band migrating in the 130 kDa region.
Holgersson
J
J
Department of Medical Biochemistry, University of Göteborg, Sweden.
Breimer
M E
ME
Jacobsson
A
A
Svensson
L
L
Ulfvin
A
A
Samuelsson
B E
BE
D000017:ABO Blood-Group System / Q000097:blood* / Q000276:immunology; D016913:Blood Component Transfusion; D001792:Blood Platelets / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D006017:Glycolipids / Q000097:blood*; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D007519:Isoantigens / Q000096:biosynthesis / Q000097:blood / Q000378:metabolism
D000017:ABO Blood-Group System; D006017:Glycolipids; D006023:Glycoproteins; D007519:Isoantigens