Interleukin 5 and interleukin 3 induce serine and tyrosine phosphorylations of several cellular proteins in an interleukin 5-dependent cell line.
Murine interleukin 5 (IL-5), a lymphokine produced by helper T cells, is involved in the regulation of growth and differentiation of B cells and eosinophils. To elucidate the intracellular events via the IL-5 receptor, we have investigated IL-5-induced protein phosphorylation in an IL-5-dependent murine early B-lineage cell line (T88-M). The rapid phosphorylation of a 60-kDa protein at serine residues and of 140-, 92-, 53-, 48-, and 45-kDa proteins at tyrosine residues were induced by the stimulation of T88-M cells with IL-5. T88-M cells were found to proliferate in response to IL-3 as well as IL-5, and the phosphoproteins in T88-M cells could be induced by IL-3 stimulation in a similar manner of those induced by IL-5. Thus, these results suggest that the protein phosphorylation events in T88-M cells may be shared by both pathways via the IL-5 receptor and the IL-3 receptor.
Murata
Y
Y
Department of Biology, Kumamoto University Medical School, Japan.
Yamaguchi
N
N
Hitoshi
Y
Y
Tominaga
A
A
Takatsu
K
K
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D007377:Interleukin-3 / Q000494:pharmacology*; D015848:Interleukin-5 / Q000494:pharmacology*; D007700:Kinetics; D008213:Lymphocyte Activation; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D011971:Receptors, Immunologic / Q000378:metabolism; D018123:Receptors, Interleukin; D016185:Receptors, Interleukin-3 / Q000378:metabolism; D053649:Receptors, Interleukin-5; D012694:Serine / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism*
D007377:Interleukin-3; D015848:Interleukin-5; D010750:Phosphoproteins; D011971:Receptors, Immunologic; D018123:Receptors, Interleukin; D016185:Receptors, Interleukin-3; D053649:Receptors, Interleukin-5; D014443:Tyrosine; D012694:Serine