The testicular receptor for follicle stimulating hormone: structure and functional expression of cloned cDNA.
Cloned cDNA encoding the rat Sertoli cell receptor for FSH was isolated from a cognate library and functionally expressed in cultured mammalian cells. The FSH receptor (FSH-R), as predicted from the cDNA, is a single 75K polypeptide with a 348 residue extracellular domain which contains three N-linked glycosylation sites. This domain is connected to a structure containing seven putative transmembrane segments which displays sequence similarity to G protein-coupled receptors. Thus, the FSH-R is identical in its structural design to the LH/CG receptor (LH/CG-R). Furthermore, both receptors display 50% sequence similarity in their large extracellular domains and 80% identity across the seven transmembrane segments. Expression of the cloned cDNA in mammalian cells conferred FSH-dependent cAMP accumulation. The selectivity for FSH is attested by the fact that the related human glycoprotein hormones human CG and human TSH do not stimulate adenylyl cyclase in FSH-R expressing cells even when these hormones are present at high concentrations.
Sprengel
R
R
University Heidelberg, Zentrum für Molekulare Biologie, Federal Republic of Germany.
Braun
T
T
Nikolics
K
K
Segaloff
D L
DL
Seeburg
P H
PH
D000262:Adenylyl Cyclases / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004789:Enzyme Activation; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D011487:Protein Conformation; D051381:Rats; D011962:Receptors, FSH / Q000096:biosynthesis / Q000235:genetics*; D011974:Receptors, LH / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D012708:Sertoli Cells / Q000737:chemistry*
D011962:Receptors, FSH; D011974:Receptors, LH; D011993:Recombinant Fusion Proteins; D005640:Follicle Stimulating Hormone; D004247:DNA; D000262:Adenylyl Cyclases