Inhibition kinetics of cationic drugs on N'-methylnicotinamide uptake by brush border membrane vesicles from the dog kidney cortex.
The effect of cationic drugs on the uptake of the prototypical organic cation N'-methylnicotinamide has been evaluated. Using purified brush border membrane vesicles prepared from dog kidney cortex and applying a rapid Millipore filtration technique, cationic drugs apparent inhibitory constants (Ki) were calculated from kinetic analysis of N'-methylnicotinamide uptake corrected for noncarrier-mediated transport (10 s uptake; outwardly directed H+ gradient; pH 7.4, 25 degrees C). All of the cationic drugs tested exhibited competitive inhibition of N'-methylnicotinamide uptake suggesting that they all share the organic base transport system at the renal proximal tubule. The Ki values were as follows, in order of decreasing apparent affinity: quinidine (0.7 microM), trimehoprim (1.3 microM), cimetidine (2.0 microM), famotidine (3.0 microM), quinine (7.0 microM), amiloride (5.8 microM), procainamide (21 microM), and nizatidine (30 microM). The different relative affinities of the drugs for the organic base transport system may explain the mutual competition for renal tubular secretion observed when cationic drugs are administered concurrently in vivo, e.g., trimethoprim--procainamide and cimetidine--procainamide. The approach outlined in the present study should prove useful to predict complex drug interactions in clinical pharmacology.
Bendayan
R
R
Faculty of Pharmacy, University of Toronto, Ont., Canada.
Sellers
E M
EM
Silverman
M
M
D000818:Animals; D001693:Biological Transport, Active; D002412:Cations / Q000494:pharmacology; D004285:Dogs; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D007684:Kidney Tubules / Q000378:metabolism; D007700:Kinetics; D008871:Microvilli / Q000187:drug effects / Q000378:metabolism; D009536:Niacinamide / Q000031:analogs & derivatives* / Q000378:metabolism; D011506:Proteins / Q000378:metabolism
D002412:Cations; D011506:Proteins; D009536:Niacinamide; C024058:N(1)-methylnicotinamide