title: cis elements and trans-acting factors involved in dimer formation of murine leukemia virus RNA.
abstract: The genetic material of all retroviruses examined so far consists of two identical RNA molecules joined at their 5' ends by the dimer linkage structure (DLS). Since the precise location of the DLS as well as the mechanism and role(s) of RNA dimerization remain unclear, we analyzed the dimerization process of Moloney murine leukemia virus (MoMuLV) genomic RNA. For this purpose we derived an in vitro model for RNA dimerization. By using this model, murine leukemia virus RNA was shown to form dimeric molecules. Deletion mutagenesis in the 620-nucleotide leader of MoMuLV RNA showed that the dimer promoting sequences are located within the encapsidation element Psi between positions 215 and 420. Furthermore, hybridization assays in which DNA oligomers were used to probe monomer and dimer forms of MoMuLV RNA indicated that the DLS probably maps between positions 280 and 330 from the RNA 5' end. Also, retroviral nucleocapsid protein was shown to catalyze dimerization of MoMuLV RNA and to be tightly bound to genomic dimer RNA in virions. These results suggest that MoMuLV RNA dimerization and encapsidation are probably controlled by the same cis element, Psi, and trans-acting factor, nucleocapsid protein, and thus might be linked during virion formation.
authors: Labo-Retro, Centre de Recherche de Biochimie et Génétique Cellulaires du C.N.R.S., Toulouse, France.
authors: A C
authors: AC
authors: Prats
authors: C
authors: C
authors: Roy
authors: P A
authors: PA
authors: Wang
authors: M
authors: M
authors: Erard
authors: V
authors: V
authors: Housset
authors: C
authors: C
authors: Gabus
authors: C
authors: C
authors: Paoletti
authors: J L
authors: JL
authors: Darlix
mesh_terms: D001358:Avian Sarcoma Viruses / Q000235:genetics; D001483:Base Sequence; D002213:Capsid / Q000378:metabolism; D005809:Genes, Regulator; D005814:Genes, Viral; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000235:genetics*; D009690:Nucleic Acid Conformation; D010957:Plasmids; D012330:RNA, Double-Stranded / Q000235:genetics* / Q000648:ultrastructure; D012367:RNA, Viral / Q000235:genetics* / Q000648:ultrastructure; D015183:Restriction Mapping; D015533:Transcriptional Activation; D014758:Viral Core Proteins / Q000378:metabolism
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014758:Viral Core Proteins