title: Two forms of the rat D2 dopamine receptor as revealed by the polymerase chain reaction.
abstract: We have used the polymerase chain reaction technique (PCR) to clone the cDNA of the D2 dopamine receptor from rat striatal mRNA. Two major PCR products were produced; one product was identical to a previously published rat cDNA, while the other, more abundant product differed only by an 87-nucleotide insert located in the region of the putative third cytoplasmic loop of the D2 receptor. A PCR approach for determining message abundance was used to determine the relative message abundance of the two forms of the D2 receptor in a variety of tissues. Possible implications of the two forms of the D2 receptor for dopamine-mediated signal transduction are discussed.
authors: Neuroscience Research Division, Abbott Laboratories, Abbott Park, IL 60064.
authors: D D
authors: DD
authors: Rao
authors: J
authors: J
authors: McKelvy
authors: J
authors: J
authors: Kebabian
authors: R G
authors: RG
authors: MacKenzie
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D003342:Corpus Striatum / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D009928:Organ Specificity; D010902:Pituitary Gland / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011954:Receptors, Dopamine / Q000235:genetics* / Q000502:physiology; D017448:Receptors, Dopamine D2; D015183:Restriction Mapping; D015398:Signal Transduction
chemical_list: D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D011954:Receptors, Dopamine; D017448:Receptors, Dopamine D2