[Rapid isolation of cell-type specific DNA binding proteins].
We had previously found a cell-type specific DNA-binding protein of approximately 150,000 using the southwestern blot technique which was found to be a regulatory factor for activation of N-ras oncogene. In this paper, we describe a modified gel elution method for isolation of this protein using mini-slab gel electrophoresis and silver staining for identification. This method is rapid, economical and preserves the DNA-binding activity of the protein as determined by nitrocellulose filter-binding assay and mobility-shift assay. It is suitable for isolation of proteins in small quantities.
Wang
Z
Z
Institute of Basic Medical Sciences, Beijing.
D004268:DNA-Binding Proteins / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel
D004268:DNA-Binding Proteins