Colorimetric assay of HIV-1 proteinase suitable for high-capacity screening.
The cleavage of substrate peptides by HIV-1 proteinase can be monitored by reaction of exposed amino groups with 2,4,6-trinitrobenzenesulfonic acid to yield yellow-colored products. This method provides a high-capacity assay of the enzyme which can be performed on microtiter plates.
Billich
A
A
Sandoz Forschungsinstitut, Vienna, Austria.
Winkler
G
G
D000595:Amino Acid Sequence; D003124:Colorimetry / Q000379:methods*; D016333:HIV Protease / Q000378:metabolism*; D015497:HIV-1 / Q000201:enzymology*; D007700:Kinetics; D008969:Molecular Sequence Data
D016333:HIV Protease