Sialylated carbohydrate chains of recombinant human glycoproteins expressed in Chinese hamster ovary cells contain traces of N-glycolylneuraminic acid.
HPLC analysis of sialic acids released from recombinant variants of human tissue plasminogen activator, human chimeric plasminogen activator, human erythropoietin, and human follitropin, expressed in Chinese hamster ovary cells, demonstrates for each glycoprotein the presence of N-acetylneuraminic acid and N-glycolylneuraminic acid in a ratio of 97:3. Structural analysis by 500 MHz1H-NMR spectroscopy, of the enzymatically released N-linked carbohydrate chains of chimeric plasminogen activator and of erythropoietin, showed that alpha 2-3 linked N-glycolylneuraminic acid can occur in different N-acetyllactosamine type antennary structures.
Hokke
C H
CH
Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.
Bergwerff
A A
AA
van Dedem
G W
GW
van Oostrum
J
J
Kamerling
J P
JP
Vliegenthart
J F
JF
D000818:Animals; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D003412:Cricetulus; D004921:Erythropoietin / Q000737:chemistry; D005260:Female; D006023:Glycoproteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009438:Neuraminic Acids / Q000032:analysis*; D010053:Ovary; D011994:Recombinant Proteins / Q000737:chemistry*; D010959:Tissue Plasminogen Activator / Q000737:chemistry
D006023:Glycoproteins; D009438:Neuraminic Acids; D011994:Recombinant Proteins; D004921:Erythropoietin; C032592:N-glycolylneuraminic acid; D010959:Tissue Plasminogen Activator