title: Performance characteristics of mammalian cell culture process operating continuously with protein-free medium.
abstract: In this communication we briefly describe a continuous industrial process for the production of Tissue Plasminogen Activator (tPA), operating with a standard, basal medium (DME/F12). Process data of two culture runs has been chosen to present a performance profile over a production period of more than 3 mo. Developmental aspects of glucose utilization (as a process parameter) are briefly discussed.
authors: Bio-Response, Inc., Hayward, CA 94545.
authors: R
authors: R
authors: Bliem
authors: R
authors: R
authors: Oakley
authors: K
authors: K
authors: Matsuoka
authors: V
authors: V
authors: Taiariol
mesh_terms: D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D006801:Humans; D008545:Melanoma; D010100:Oxygen / Q000378:metabolism; D010959:Tissue Plasminogen Activator / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
chemical_list: D003470:Culture Media; D010959:Tissue Plasminogen Activator; D005947:Glucose; D010100:Oxygen