Neolacto (type-2 chain)-sialoautoantigens recognized by human cold agglutinins.
The antigens Fl and Vo, recognized by cold agglutinins, are protease-resistant differentiation antigens like I and i antigens. In contrast to Ii, Fl and Vo are sialidase-susceptible antigens like the protease-resistant Gd antigens. Because I/i antigens are branched/linear type-2 (poly-N-acetyllactosamine or neolacto series) chains which are cleaved from red cells by endo-beta-galactosidase, the effect of the enzyme on Fl, Vo and Gd antigens was studied. Fl and Gd antigens, previously shown to be sialylated branched (Fl), and linear and branched (Gd) type-2 sequences, were not inactivated on red cells by the enzyme, whereas Vo antigenicity was completely abolished. The data suggest that Vo antigen is expressed on sialylated linear poly-N-acetyllactosamine sequence.
Roelcke
D
D
Institute of Immunology, University of Heidelberg, FRG.
Hengge
U
U
Kirschfink
M
M
D000373:Agglutinins / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D002240:Carbohydrate Sequence; D003080:Cold Temperature; D004912:Erythrocytes / Q000276:immunology*; D006026:Glycoside Hydrolases; D006801:Humans; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000276:immunology*; D012794:Sialic Acids / Q000276:immunology*; D001616:beta-Galactosidase
D000373:Agglutinins; D001324:Autoantigens; D011134:Polysaccharides; D012794:Sialic Acids; C037199:poly-N-acetyllactosamine; D006026:Glycoside Hydrolases; C026093:keratan-sulfate endo-1,4-beta-galactosidase; D001616:beta-Galactosidase