Distribution of glycosyltransferase in bovine eyes.
The activities of sialyl-, fucosyl- and galactosyltransferase were measured in membrane preparations from bovine eyes. Radioactive sugars from CMP-N-neuraminic acid, GDP-fucose and UDP-galactose were incorporated into the endogenous and exogenous acceptors. The enzymatic activities of all three glycosyltransferases were found to be high in the neural retina and retinal pigment epithelium, moderate in the cornea and uvea, and low in the lens and sclera, except for a high galactosyltransferase activity in the cornea.
Unoki
K
K
Department of Ophthalmology, Kagoshima University Faculty of Medicine, Japan.
Uehara
F
F
Muramatsu
T
T
D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000201:enzymology; D005123:Eye / Q000201:enzymology*; D005647:Fucosyltransferases / Q000378:metabolism*; D005700:Galactosyltransferases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012799:Sialyltransferases / Q000378:metabolism*; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
D005647:Fucosyltransferases; D005700:Galactosyltransferases; D012799:Sialyltransferases; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase