Effect of atrial natriuretic peptide on the adenylate cyclase activity in the anterior and neurointermediate pituitary lobes of rats.
The effect of two atrial natriuretic peptides, rat ANP (3-28) and synthetic ANP (Arg101-Tyr126), on pituitary adenylate cyclase activity was measured in rats. Neither one was effective on basal, forskolin- or guanylylimidodiphosphate (GMP-PNP)-stimulated adenylate cyclase levels measured in homogenates of anterior and neurointermediate pituitary lobes. High concentrations (10(-7) and 10(-6) M) of ANPs further stimulate NaF-induced high adenylate cyclase activity - ANP (3-28) in both lobes, ANP (Arg101-Tyr126) only in the anterior lobe-but they were without effect in 10(-8) M or lower concentrations. These data indicate that ANP may not influence adenylate cyclase/cAMP system in the rat pituitary gland.
Geiger
H
H
Department of Nephrology, University of Würzburg, FRG.
Bahner
U
U
Palkovits
M
M
Hempel
K
K
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000008:administration & dosage / Q000494:pharmacology*; D005576:Colforsin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D008297:Male; D010446:Peptide Fragments / Q000008:administration & dosage / Q000494:pharmacology; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains
D010446:Peptide Fragments; C049490:atrial natriuretic factor (3-28); D005576:Colforsin; D009320:Atrial Natriuretic Factor; C043970:atrial natriuretic factor 26, rat; D000262:Adenylyl Cyclases