Haemangioblastoma: histological and immunohistological study of an enigmatic cerebellar tumour.
Paraffin-embedded blocks of 36 cerebellar haemangioblastomas were reacted with a panel of antibodies including glial fibrillary acidic protein, vimentin, epithelial membrane antigen, cytokeratin, Factor VIII, a neuroendocrine marker and with Ulex europaeus. agglutinin The main histological features, apart from the characteristic large abnormal vessels, were a prominent reticulin network, a cystic architecture and cellular and nuclear polymorphism. Two cell types were identified: endothelial and stromal. Twenty tumours were positive for glial fibrillary acidic protein because of included or reactive astrocytes as well as positive stromal cells. Vimentin was positive in all tumours with a diffuse distribution and a somatic pattern; blood vessels, stromal cells and reactive astrocytes were strongly positive. Factor VIII and Ulex europaeus agglutinin reactivity were present in a similar pattern of staining in endothelium and in five cases there were stromal cells that were positive with the latter. We were not able to ascertain the histogenesis of the stromal cell, which remains enigmatic.
Cruz-Sánchez
F F
FF
Department of Neuropathology, Radcliffe Infirmary, Oxford, UK.
Rossi
M L
ML
Rodríguez-Prados
S
S
Nakamura
N
N
Hughes
J T
JT
Coakham
H B
HB
D000293:Adolescent; D000328:Adult; D000368:Aged; D002528:Cerebellar Neoplasms / Q000378:metabolism / Q000473:pathology*; D002648:Child; D005169:Factor VIII / Q000378:metabolism; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006394:Hemangiosarcoma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D037102:Lectins; D008297:Male; D008875:Middle Aged; D037121:Plant Lectins; D014746:Vimentin / Q000378:metabolism
D005904:Glial Fibrillary Acidic Protein; D037102:Lectins; D037121:Plant Lectins; C036850:Ulex europaeus lectins; D014746:Vimentin; D005169:Factor VIII