Differences in the susceptibility of plant membrane lipids to peroxidation.
Peroxidation of three membrane lipid preparations from plants was initiated using Fe-EDTA and ascorbate and quantified as the production of aldehydes and loss of esterified fatty acids. Using liposomes prepared from commercial soybean asolecithin, the degree of peroxidation was shown to be dependent on: the free radical dose, which was varied by the ascorbate concentration; the presence of tocopherol in the liposome; the configuration, of the liposome, multilamellar or unilamellar; and time after initiation. There were dramatic interactions among these factors which led to the conclusion that in comparing the susceptibility of different membrane preparations it is essential to examine the kinetics of the peroxidation reactions. The composition of the liposome was a major determinant of the degree of peroxidation and of the type of degradative reactions initiated by the oxygen free radicals. A fresh polar lipid extract from Typha pollen had very similar fatty acid composition to the soybean asolecithin, but was more resistant to peroxidation as shown by less aldehyde production and increased retention of unsaturated fatty acids after treatment. Similarly, microsomal membranes from the crowns of non-acclimated and cold acclimated winter wheat (Triticum aestivum L.) seedlings had a much higher linolenic acid content than soybean asolecithin but was much more resistant to peroxidation. In the winter wheat microsomes, the loss of esterified fatty acids was not selective for the unsaturated fatty acids; consequently, even with 40% degradation, the degree of unsaturation in the membrane did not decrease. These different reaction mechanisms which occur in plant membranes may explain why measurements of fatty acid unsaturation fail to detect peroxidative reactions during processes such as senescence, aging and environmental stress.
McKersie
B D
BD
Department of Plant Physiology, Agricultural University, Wageningen, The Netherlands.
Hoekstra
F A
FA
Krieg
L C
LC
D000447:Aldehydes / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D004492:Edetic Acid / Q000378:metabolism; D004951:Esterification; D005227:Fatty Acids / Q000378:metabolism; D005290:Ferric Compounds / Q000378:metabolism; D005609:Free Radicals; D007425:Intracellular Membranes / Q000378:metabolism; D015227:Lipid Peroxidation; D008081:Liposomes / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D010944:Plants / Q000032:analysis*; D011058:Pollen / Q000032:analysis; D013025:Glycine max / Q000032:analysis; D014908:Triticum / Q000648:ultrastructure; D014810:Vitamin E / Q000494:pharmacology
D000447:Aldehydes; D005227:Fatty Acids; D005290:Ferric Compounds; D005609:Free Radicals; D008081:Liposomes; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D014810:Vitamin E; D008315:Malondialdehyde; D004492:Edetic Acid; C019179:Fe(III)-EDTA; D001205:Ascorbic Acid; D010100:Oxygen