A highly immunogenic tumor transfected with a murine transforming growth factor type beta 1 cDNA escapes immune surveillance.
A highly immunogenic C3H-derived UV-induced tumor was cotransfected with a murine transforming growth factor type beta 1 (TGF-beta 1) cDNA and a neomycin-resistance gene. Stable clones were isolated and used in vitro and in vivo to determine the effects of endogenously produced TGF-beta on cytolytic T-lymphocyte (CTL) responses. Tumor cells producing TGF-beta, though retaining expression for class I major histocompatibility complex molecules and the tumor-specific antigen, did not stimulate primary CTL responses in vitro and were not effective in vivo for directly stimulating primary CTL or in priming for CTL responses. Furthermore, TGF-beta-producing tumors grew progressively in transiently immunosuppressed mice without losing the tumor antigen; thus, TGF-beta produced by tumors may promote escape from immune surveillance.
Torre-Amione
G
G
Department of Pathology, University of Chicago, IL 60637.
Beauchamp
R D
RD
Koeppen
H
H
Park
B H
BH
Schreiber
H
H
Moses
H L
HL
Rowley
D A
DA
D000818:Animals; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D005091:Exons; D005260:Female; D005354:Fibrosarcoma / Q000276:immunology*; D007111:Immunity, Cellular; D007157:Immunologic Surveillance; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008809:Mice, Inbred C3H; D008819:Mice, Nude; D009381:Neoplasms, Radiation-Induced / Q000276:immunology; D010957:Plasmids; D015183:Restriction Mapping; D012513:Sarcoma, Experimental / Q000276:immunology*; D014162:Transfection; D015291:Transforming Growth Factors / Q000235:genetics / Q000276:immunology*; D014466:Ultraviolet Rays
D015291:Transforming Growth Factors