[Does induced Heinz body formation lead to the liberation of IgG receptors on the erythrocyte membrane?].
Immunofluorescence microscopical and biochemical studies led other authors to the conclusion that the formation of membrane-bound Heinz bodies at these parts of the plasmalemma of erythrocytes leads to clustering of band 3-protein and increased binding of IgG. We failed to detect immunocytochemically an increased IgG binding over phenylhydrazine induced membrane-bound Heinz bodies in otherwise intact erythrocytes using transmission electronmicroscopy and we also did not notice any clustering of intramembraneous particles over the numerous Heinz bodies by means of freeze etching. Especially at higher phenylhydrazine concentrations the erythrocytes show formation of vesicles and hemolysis accompanied by increased IgG binding and clustering of the intramembraneous particles. Photographs of such cells obtained by immunofluorescence microscopy are very similar to the pictures known from the literature.
Linss
W
W
Institut für Anatomie, Friedrich-Schiller-Universität Jena.
Halbhuber
K J
KJ
Werner
J
J
Zimmermann
N
N
Meyer
H W
HW
D001457:Anion Exchange Protein 1, Erythrocyte / Q000502:physiology / Q000648:ultrastructure; D000943:Antigens, Differentiation / Q000502:physiology*; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000276:immunology / Q000648:ultrastructure*; D005613:Freeze Etching; D006366:Heinz Bodies / Q000187:drug effects / Q000648:ultrastructure*; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D010659:Phenylhydrazines / Q000494:pharmacology*; D011961:Receptors, Fc / Q000502:physiology* / Q000648:ultrastructure; D017452:Receptors, IgG
D001457:Anion Exchange Protein 1, Erythrocyte; D000943:Antigens, Differentiation; D007074:Immunoglobulin G; D010659:Phenylhydrazines; D011961:Receptors, Fc; D017452:Receptors, IgG
Führt induzierte Heinz-Körper-Bildung zur Freisetzung von IgG-Rezeptoren an der Erythrozytenmembran?