[Induction of quiescence by differentiating agents].
The growth fraction of cancer cells, estimated by the monoclonal antibody Ki-67 labelling, and DNA content were determined simultaneously en K562 human leukemic cells by flow cytometry. Adriamycin, aclacinomycin A and fagaronine induced differentiation, as assessed by benzidine staining and glycophorin A expression. These drugs decreases the fraction of Ki-67 positive cells, Ki-67 negative cells displayed a G1, but also a G2 and a S DNA content in different proportions, indicating that induction of quiescent cells by differentiating agents is not a uniform process and is worthy of interest.
Gorisse
M C
MC
Institut J. Godinot, GIBSA, Reims.
Carpentier
Y
Y
Desoize
B
B
D015250:Aclarubicin / Q000494:pharmacology*; D000470:Alkaloids / Q000494:pharmacology*; D000911:Antibodies, Monoclonal / Q000032:analysis; D000970:Antineoplastic Agents / Q000494:pharmacology*; D053119:Benzophenanthridines; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced* / Q000235:genetics / Q000276:immunology; D004247:DNA / Q000032:analysis; D004317:Doxorubicin / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D007938:Leukemia / Q000473:pathology; D010617:Phenanthridines
D000470:Alkaloids; D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; D053119:Benzophenanthridines; D010617:Phenanthridines; C002066:fagaronine; D015250:Aclarubicin; D004317:Doxorubicin; D004247:DNA
Induction de quiescence par des agents différenciants.