Fast plaque sizing and some applications of the technique in antibody feedback experiments.
A method has been devised to make rapid assessments of plaque size simultaneously with plaque enumeration. The method depends on an inexpensive and simple interfacing of a weighted counter to a microcomputer. Comparisons made between relative (weighted counter) and absolute (photographic) assessments of plaque size, show that the relationship is not linear. It has been shown that assay conditions must be rigidly controlled for valid size comparisons to be made. IgM and IgG PFC specific for sheep RBC, burro RBC and TNP-, in primary and secondary, active and adoptive responses, were used in these experiments. An application of the method in antibody feedback experiments is described.
Dresser
D W
DW
National Institute for Medical Research, London, U.K.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology; D005246:Feedback; D005260:Female; D005362:Ficoll / Q000031:analogs & derivatives / Q000276:immunology; D006462:Hemolytic Plaque Technique / Q000295:instrumentation; D007114:Immunization / Q000379:methods; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008838:Microcomputers; D010527:Perissodactyla; D010781:Photography; D011817:Rabbits; D012756:Sheep; D013047:Specific Pathogen-Free Organisms; D014301:Trinitrobenzenes / Q000276:immunology
D007074:Immunoglobulin G; D007075:Immunoglobulin M; C020529:TNP-ficoll; D014301:Trinitrobenzenes; D005362:Ficoll