Quantitative morphological study of Langerhans cells by computerized image analysis.
ATPase-stained normal and topical nitrogen mustard treated Langerhans cells (LC) in guinea pig epidermis were studied quantitatively and morphologically by computerized image analysis. Significant correlation and linear regression were found between the perimeter and square root of its area for both cytoplasm and nucleus; a theoretical basis was thus given for morphological identification of LC. The directly measured and derived geometric parameters indicated not only substantial alteration of cytoplasm morphology of post-treated LC such as cytoplasmatosis and dendrites reduction but also a potential change of nuclear size and functional status. We conclude that quantitative morphological analysis is an objective, precise and practical method for LC research.
Xie
Y
Y
Department of Dermatology, Changhai Hospital, Second Military Medical College.
Zhou
D Y
DY
Zheng
M R
MR
Zhao
H J
HJ
D000818:Animals; D006168:Guinea Pigs; D007091:Image Processing, Computer-Assisted; D007801:Langerhans Cells / Q000166:cytology / Q000648:ultrastructure*; D008297:Male; D012867:Skin / Q000166:cytology*