In vitro and in vivo onco-suppressor activity of normal cells on cells transformed with the H-ras1 oncogene.
We have investigated mixed cultures of "normal" early passage Balb/c embryo cells and Balb/c 3T3 cells transformed by the human T24 H-ras1 oncogene. The presence of an excess of "Normal" cells could suppress the phenotype of transformed cells in vitro. A similar type of suppression by normal cells could be shown in vivo on tumors induced by Balb/c 3T3 transformed cells. The suppressing effect of normal cells on T24 H-ras1 transformed cells could also be demonstrated by DNA synthesis inhibition experiments. It is suggested that normal cells could either carry or induce tumor inhibitory substances.
Kakkanas
A
A
Hellenic Pasteur Institute, Athens, Greece.
Spandidos
D A
DA
D000818:Animals; D002450:Cell Communication; D002455:Cell Division; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D002478:Cells, Cultured; D005354:Fibrosarcoma / Q000737:chemistry / Q000235:genetics; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C / Q000196:embryology; D009363:Neoplasm Proteins / Q000032:analysis; D009368:Neoplasm Transplantation; D009857:Oncogenes; D010641:Phenotype; D016283:Proto-Oncogene Proteins p21(ras) / Q000032:analysis / Q000235:genetics* / Q000502:physiology
D009363:Neoplasm Proteins; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)