cDNA cloning and sequencing for the import precursor of subunit B in H(+)-ATP synthase from rat mitochondria.
The nucleotide sequence of the import precursor of subunit b of rat liver H(+)-ATP synthase has been determined from a recombinant cDNA clone isolated by screening a rat liver cDNA library with a probe DNA. The sequence was composed of 1,124 nucleotides including a coding region for the import precursor of subunit b and noncoding regions of both the 5'- and 3'-sides. The import precursor of subunit b and its mature polypeptide deduced from the open reading frame consisted of 256 and 214 amino acid residues with a molecular weight of 28,867 and 24,628, respectively. The presequence of 42 amino acids could be the import signal peptide which serves to direct the protein into the mitochondrial matrix.
Tsurumi
C
C
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Yoshihara
Y
Y
Osaka
F
F
Yamada
F
F
Tani
I
I
Higuti
T
T
Shimizu
M
M
Oeda
K
K
Ohkawa
H
H
Toda
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D015723:Gene Library; D008930:Mitochondria, Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000032:analysis / Q000235:genetics*; D006180:Proton-Translocating ATPases / Q000032:analysis / Q000235:genetics*; D051381:Rats
D021382:Protein Sorting Signals; D004247:DNA; D006180:Proton-Translocating ATPases