L-asparaginase II of Escherichia coli K-12: cloning, mapping and sequencing of the ansB gene.
The Escherichia coli gene ansB, encoding the chemotherapeutic enzyme L-asparaginase II, has been cloned, using a strategy based on the polymerase chain reaction, and sequenced. The amino acid (aa) sequence differs in eleven positions from the data previously derived by direct aa sequencing. A cleavable secretory signal peptide precedes the N terminus of the mature protein. The ansB gene maps to position 3114 kb on the physical map of E. coli [Kohara et al., Cell 50 (1987) 495-508], corresponding to approx. 63.8 min on the genetic map.
Bonthron
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DT
Department of Medicine, Western General Hospital, Edinburgh University, U.K.
D000595:Amino Acid Sequence; D001215:Asparaginase / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D021382:Protein Sorting Signals / Q000235:genetics; D015183:Restriction Mapping
D007527:Isoenzymes; D015345:Oligonucleotide Probes; D021382:Protein Sorting Signals; D001215:Asparaginase