Preservation of ruminal bacterium capsules by using lysine in the electron microscopy fixative.
Ruminal bacteria from axenic cultures of Ruminococcus flavefaciens FD1, Butyrivibrio fibrisolvens 49, and bacterial types from the ruminal ecosystem that were fixed with 50 mM lysine (l-lysine hydrochloride) added to glutaraldehyde had better-preserved capsules and extracellular material than bacteria fixed without lysine.
Akin
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Richard B. Russell Agricultural Research Center, U.S. Department of Agriculture, Athens, Georgia 30613.
Rigsby
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D000818:Animals; D001419:Bacteria / Q000648:ultrastructure*; D001431:Bacteriological Techniques; D005404:Fixatives; D006089:Gram-Negative Anaerobic Bacteria / Q000648:ultrastructure; D008239:Lysine; D008854:Microscopy, Electron; D010459:Peptococcaceae / Q000648:ultrastructure; D012417:Rumen / Q000382:microbiology*
D005404:Fixatives; D008239:Lysine