A method for preparing biologically active aqueous cyclosporin A solutions avoiding the use of detergents or organic solvents.
The immunosuppressive agent cyclosporin A (CsA) is highly hydrophobic and is, therefore, being taken for in vitro use directly from ethanolic stock solutions. In the present study it is demonstrated that ethanol itself affects several immunological activities in vitro, thus interfering with the immunosuppressive effects of CsA. As an alternative, it is demonstrated that CsA can be solubilized and retain its activity in aqueous solutions of polyvinyl pyrrolidone (PVP). Unlike ethanol, PVP has no effect on the various immunological activities in vitro and therefore the immunosuppressive effects of CsA can be evaluated without interference.
Yonish-Rouach
E
E
Department of Molecular Genetics and Virology, Weizmann Institute of Science, Rehovot, Israel.
Shinitzky
M
M
Rubinstein
M
M
D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis; D002460:Cell Line / Q000187:drug effects; D003524:Cyclosporins / Q000737:chemistry* / Q000494:pharmacology; D003902:Detergents; D004790:Enzyme Induction / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology; D016259:Genes, myc / Q000187:drug effects; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects; D008222:Lymphokines / Q000096:biosynthesis; D011205:Povidone / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis; D012995:Solubility; D012997:Solvents; D014407:Tumor Cells, Cultured / Q000187:drug effects
D003524:Cyclosporins; D003902:Detergents; D008222:Lymphokines; D012333:RNA, Messenger; D012997:Solvents; D000431:Ethanol; D007371:Interferon-gamma; D015088:2',5'-Oligoadenylate Synthetase; D011205:Povidone