Flow cytometric analysis of cell cycle kinetics of anagen scalp hair correlated with plasma androgens in hirsutism.
Cell kinetics of anagen scalp hair bulbs obtained from hirsute (n = 13) as well as healthy (n = 10) females were analysed by DNA-flow cytometry. The cell cycle kinetics in hirsutism revealed a significant increase of S-phase cells (10.2%) and a significant decrease of G0/1-phase cells (80.7%) compared with healthy females (S-phase 7.5%, G0/1 phase 86%). Moreover, dehydroepiandrosterone sulfate (DHEA-S) levels and cell cycle kinetics obtained from the hirsute females yielded a strong correlation between the height of S-phase percentages and DHEA-S values, whereas no correlation could be proved between testosterone levels and DNA-FCM data. Therefore, the weak androgen DHEA-S is assumed to be one hormonal factor influencing the cellular growth kinetics of hair bulbs in androgen-sensitive scalp areas.
Kiesewetter
F
F
Department of Dermatology, University of Erlangen-Nürnberg, FRG.
Schell
H
H
Seidel
C
C
D000328:Adult; D002453:Cell Cycle / Q000502:physiology; D002455:Cell Division; D004247:DNA / Q000032:analysis; D003687:Dehydroepiandrosterone / Q000031:analogs & derivatives* / Q000097:blood / Q000502:physiology; D019314:Dehydroepiandrosterone Sulfate; D005260:Female; D005434:Flow Cytometry; D006197:Hair / Q000254:growth & development*; D006628:Hirsutism / Q000097:blood / Q000473:pathology*; D006801:Humans; D012535:Scalp / Q000473:pathology; D013739:Testosterone / Q000097:blood / Q000502:physiology*
D013739:Testosterone; D003687:Dehydroepiandrosterone; D019314:Dehydroepiandrosterone Sulfate; D004247:DNA