title: Stimulation of a neutral cholesteryl ester hydrolase by cAMP system in P388D1 macrophages.
abstract: Cholesteryl ester laden foam cells in atherosclerotic lesions derive, in part, from macrophages. Mobilization of stored cholesteryl esters involves hydrolysis by a neutral cholesteryl ester hydrolase. Incubation of intact P388D1 macrophages with dibutyryl cAMP in the presence of 1-methyl-3-isobutylxanthine resulted in a dose-dependent increase in neutral cholesteryl ester hydrolase activity of up to 50% (ED50 = 0.1 mM). Incubation with prostaglandin E1 in the presence of 1-methyl-3-isobutylxanthine also increased neutral cholesterol ester hydrolase activity by about 50%. In cell-free preparation, cAMP-dependent protein kinase caused about a 2-fold activation of the neutral cholesteryl ester hydrolase. Activation was blocked by protein kinase inhibitor. These data suggest that the P388D1 macrophage may be a useful model for studying the hormonal regulation of cholesteryl ester mobilization in macrophage-derived foam cells.
authors: Department of Medicine, University of California, San Diego, La Jolla.
authors: D I
authors: DI
authors: Goldberg
authors: J C
authors: JC
authors: Khoo
mesh_terms: D000527:Alprostadil / Q000494:pharmacology; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism*; D004789:Enzyme Activation; D008264:Macrophages / Q000201:enzymology*; D051379:Mice; D047428:Protein Kinase Inhibitors; D002787:Sterol Esterase / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000201:enzymology / Q000378:metabolism
chemical_list: D047428:Protein Kinase Inhibitors; D003994:Bucladesine; D000242:Cyclic AMP; D002265:Carboxylic Ester Hydrolases; D002787:Sterol Esterase; D000527:Alprostadil