[Establishment of Wg3h-neo cell line with two genetic markers].
Plasmid PSV2-neo DNA containing neo gene (PSV2 neo) was successfully transferred into Wg3h cells, a HGPRT-Chinese hamster fibroblast cell line, by calcium phosphate DNA co-precipitating technique. Cells carrying two selectable markers (AGR and G418R) were thus obtained and passed for more than 40 generations, and designated as Wg3h-neo cell line. The existence of PSV2-neo sequences in the Wg3h-neo genome was confirmed by dot-blot hybridization with PSV2-neo DNA probe. Comparative studies showed that no significant difference in morphology, growth rate or genetic characteristics could be found between the Wg3h and Wg3h-neo cell lines.
Han
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Institute of Basic Medical Sciences, Beijing.
Xue
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D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D005347:Fibroblasts / Q000166:cytology; D005798:Genes, Bacterial / Q000235:genetics*; D005819:Genetic Markers; D010957:Plasmids; D014162:Transfection / Q000235:genetics*
D005819:Genetic Markers