The use of tandem mass spectrometry for the differentiation of bile acid isomers and for the identification of bile acids in biological extracts.
Tandem mass spectrometric (MS/MS) techniques hae been widely used for the differentiation of isomeric compounds, since their spectra may show differences sufficient to distinguish between them. There are several different ways by which the MS/MS data can be obtained depending on the energies of the ions and the collisions. In this paper MS/MS spectra have been obtained for a group of isomeric bile acids using: 1, low-energy ions and low-energy collisions in a triple quadrupole mass spectrometer by liquid chromatography/MS/MS; 2, high-energy ions and low-energy collisions in a hybrid mass spectrometer by fast-atom bombardment MS/MS. Liquid chromatography combined with tandem mass spectrometry (LC/MS/MS) has also been used to identify the bile acids present in biological matrices such as bile extracts.
Eckers
C
C
Smith Kline and French Research Ltd, Welwyn, Herts, UK
New
A P
AP
East
P B
PB
Haskins
N J
NJ
D000818:Animals; D001647:Bile Acids and Salts / Q000032:analysis*; D002417:Cattle; D066298:In Vitro Techniques; D007536:Isomerism; D013058:Mass Spectrometry; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013552:Swine
D001647:Bile Acids and Salts