Cloning of the listeriolysin O gene and development of specific gene probes for Listeria monocytogenes.
A clone containing 3.1 kb of Listeria DNA was selected from a gene library of Listeria monocytogenes Scott A strain. The Escherichia coli clone produced hemolysin on sheep blood agar and in sonicated extracts but very little in the culture supernatant. This 3.1-kb DNA fragment and a 650-bp HindIII fragment located within the listeriolysin gene were used as probes in a colony hybridization assay. Both probes were specific for L. monocytogenes and did not hybridize with any other Listeria strains at high stringency. Two synthetic probes, one from the 650-bp HindIII fragment and one from the carboxy-terminal region of the protein, were also specific for L. monocytogenes.
Datta
A R
AR
Division of Microbiology, Food and Drug Administration, Washington, D.C. 20204.
Wentz
B A
BA
Russell
J
J
D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins; D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics*; D006460:Hemolysin Proteins / Q000235:genetics*; D008089:Listeria monocytogenes / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D015342:DNA Probes; D004269:DNA, Bacterial; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D015345:Oligonucleotide Probes; C038705:hlyA protein, Listeria monocytogenes