Anti-proliferative effects and phenotypic alterations induced by 8-hydroxyquinoline in melanoma cell lines.
The effect of the transition metal chelator, 8-hydroxyquinoline (8-HQ), was examined on the growth and phenotype expression of B16 mouse melanoma cells. Micromolar concentrations of 8-HQ inhibited the growth of B16 cells as well as human melanoma cell lines. Removal of 8-HQ from the culture medium restored normal cell growth. Growth inhibition by 8-HQ was accompanied by phenotypic alterations that included changes in cell morphology, increased production of melanin and enhanced activities of the enzymes gamma-glutamyl transpeptidase and NADPH cytochrome c reductase. These changes might be associated with a better differentiated phenotype.
Nordenberg
J
J
Rogoff Research Institute, Beilinson Medical Center, Petah Tikva, Israel.
Novogrodsky
A
A
Beery
E
E
Patia
M
M
Wasserman
L
L
Warshawsky
A
A
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D006801:Humans; D008545:Melanoma / Q000188:drug therapy* / Q000473:pathology; D008546:Melanoma, Experimental / Q000188:drug therapy; D051379:Mice; D015125:Oxyquinoline / Q000627:therapeutic use*
D015125:Oxyquinoline