[Influence of culture conditions on the growth of murine bone marrow stromal colony-forming cells (CFU-f) in vitro].
Several factors which may influence the colony-forming efficiency of mouse CFU-f, including media, culture dishes, mouse strains, and O2 concentrations in the incubator were tested. Among them, the O2 concentration was found to be the most important factor, when a mixed gas consisting of 5% O2, 5% CO2 and 90% N2 was used to replace the usual 5% CO2 in air, the colony-forming efficiency of CFU-f increased markedly and the influence of other factors on cell growth diminished. Although the use of a lit candle to consume O2 in the air provided good conditions for colony formation of several tumor cell lines in soft agar, it is not suitable for the growth of CFU-f.
Xu
C
C
Institute of Materia Medica, Beijing.
Han
R
R
D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D010100:Oxygen / Q000032:analysis
D003470:Culture Media; D010100:Oxygen