Prostacyclin production by blood-contacting surfaces of endothelialized vascular prostheses.
This study examines prostacyclin production by blood-contacting surfaces within woven vascular prostheses of polydioxanone (PDS), polyglactin 910 (PG910), or Dacron interposed into rabbit infrarenal aortas. Grafts and normal aortic segments were explanted after 1, 3, and 6 months for pulsatile perfusion with Medium-199 for 60 minutes. Aliquots were removed serially for 6-keto-PGF1 alpha assay. After 30 minutes sodium arachidonate (10 micrograms/ml) was added. Specimens were studied by light microscopy, SEM and TEM. Patency in all three groups exceeded 90%. All three showed re-endothelialization at one month. Normal aorta produced low basal 6-keto-PGF1 alpha with a marked evanescent post arachidonate increase. Dacron did not differ from normal aorta. PG910 and PDS both produced significantly less 6-keto-PGF1 alpha post arachidonate at one month but both increased to normal by three months.
Greisler
H P
HP
Loyola University Medical Center, Maywood, IL.
Klosak
J J
JJ
McGurrin
J F
JF
Endean
E D
ED
Ellinger
J
J
Pozar
J D
JD
Henderson
S C
SC
Kim
D U
DU
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis*; D000818:Animals; D001012:Aorta, Abdominal; D001672:Biocompatible Materials; D001807:Blood Vessel Prosthesis; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006083:Graft Occlusion, Vascular / Q000517:prevention & control; D016687:Polydioxanone; D011091:Polyesters; D011093:Polyethylene Terephthalates; D011098:Polyglactin 910; D011817:Rabbits; D013997:Time Factors
D001672:Biocompatible Materials; D011091:Polyesters; D011093:Polyethylene Terephthalates; D016687:Polydioxanone; D011098:Polyglactin 910; D015121:6-Ketoprostaglandin F1 alpha