Changes of ADP-ribosylation of GTP-binding protein by pertussis toxin in human platelets during long-term treatment of manic depression with lithium carbonate.
1. The study was done to determine whether long-term treatment with lithium carbonate affected the guanosine triphosphate (GTP)-binding protein in platelet membranes from patients with manic depression. 2. It was found that adenosine diphosphate (ADP)-ribosylation of inhibitory G-proteins (40-41 kDa) by pertussis toxin, islet-activating protein in the cell membranes increased about 45% when long-term treatment with lithium carbonate was stopped. 3. These results support the idea that one site for the therapeutic effects of lithium ions is G-proteins.
Watanabe
Y
Y
Department of Pharmacology I, Osaka University School of Medicine, Japan.
Morita
H
H
Imaizumi
T
T
Takeda
M
M
Hariguchi
S
S
Nishimura
T
T
Yoshida
H
H
D000244:Adenosine Diphosphate / Q000378:metabolism*; D001714:Bipolar Disorder / Q000188:drug therapy; D001792:Blood Platelets / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism*; D006801:Humans; D008094:Lithium / Q000008:administration & dosage* / Q000627:therapeutic use; D016651:Lithium Carbonate; D037342:Pertussis Toxin; D011485:Protein Binding / Q000187:drug effects; D013997:Time Factors; D010566:Virulence Factors, Bordetella / Q000378:metabolism
D010566:Virulence Factors, Bordetella; D016651:Lithium Carbonate; D000244:Adenosine Diphosphate; D006160:Guanosine Triphosphate; D008094:Lithium; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins