Antigen presentation requires transport of MHC class I molecules from the endoplasmic reticulum.
The role of exocytosis of major histocompatibility complex (MHC) class I molecules in the presentation of antigens to mouse cytotoxic T lymphocytes (CTLs) was examined by use of a recombinant vaccinia virus that expresses the E19 glycoprotein from adenovirus. E19 blocked the presentation of vaccinia and influenza virus proteins to CTLs in a MHC class I allele-specific manner identical to its inhibition of MHC class I transport from the endoplasmic reticulum. This finding indicates that (i) the relevant parameter for antigen presentation is the rate of MHC class I molecule exocytosis, not the level of class I cell surface expression, and (ii) association of class I molecules with antigen is likely to occur within the endoplasmic reticulum.
Cox
J H
JH
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.
Yewdell
J W
JW
Eisenlohr
L C
LC
Johnson
P R
PR
Bennink
J R
JR
D017456:Adenovirus Early Proteins; D000818:Animals; D000938:Antigen-Presenting Cells / Q000187:drug effects / Q000276:immunology / Q000648:ultrastructure*; D000941:Antigens / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D000998:Antiviral Agents; D001692:Biological Transport; D020126:Brefeldin A; D002460:Cell Line; D003517:Cyclopentanes / Q000494:pharmacology; D004721:Endoplasmic Reticulum / Q000276:immunology*; D005089:Exocytosis; D006023:Glycoproteins / Q000032:analysis; D006183:H-2 Antigens / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA; D009856:Oncogene Proteins, Viral / Q000032:analysis; D009976:Orthomyxoviridae Infections / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014616:Vaccinia virus / Q000276:immunology
D017456:Adenovirus Early Proteins; D000941:Antigens; D000956:Antigens, Viral; D000998:Antiviral Agents; D003517:Cyclopentanes; D006023:Glycoproteins; D006183:H-2 Antigens; D015395:Histocompatibility Antigens Class I; D009856:Oncogene Proteins, Viral; D011994:Recombinant Proteins; D020126:Brefeldin A