Comparison of the intestinal bacteria in specific pathogen free mice from different breeders.
Specific pathogen free BALB/c mice from 3 commercial laboratory animal breeders in Japan were compared on the composition of caecal flora revealed by selective and nonselective cultivation as well as direct microscopical observation on smears, and relative caecal weight. Large differences were detected in viable counts of total bacteria and almost all bacterial groups, while direct microscopical counts which consisted mainly of fusiform bacteria were almost equal, resulting in diverse recovery rates among 3 breeders. Eubacterium and spiral shaped organisms were not detected from all breeders. There also existed variations within breeders, especially those in the number of Enterobacteriaceae. Relative caecal weight also differed among breeders, suggesting the participation of variety of caecal bacteria in determining this parameter. As these variations in bacteriological status of animals can influence experimental results, standardization of intestinal microbial flora is required.
Hirayama
K
K
Department of Veterinary Public Health, Faculty of Agriculture, University of Tokyo, Japan.
Endo
K
K
Kawamura
S
S
Mitsuoka
T
T
D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D001947:Breeding; D002432:Cecum / Q000033:anatomy & histology / Q000382:microbiology; D015169:Colony Count, Microbial; D003470:Culture Media; D004285:Dogs; D005856:Germ-Free Life; D007422:Intestines / Q000382:microbiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009929:Organ Size; D013047:Specific Pathogen-Free Organisms
D003470:Culture Media