[Diagnosis of Trypanosoma cruzi infection in opossums, naturally infected, using indirect immunofluorescence reaction].
One hundred and sixteen opossums captured in Bambuí, MG State, had an indirect fluorescent antibody test (IFAT) to detect circulating Trypanosoma cruzi antibodies. Forty-four of them (37.9%) reacted to T. cruzi, showing, titres ranging from 1:10 to 1:320. The parasite was demonstrated by parasitological examinations in 43 (97.7%) of these opossums. The IFAT presented co-positivity (97.7%) co-negativity (98.6%) and agreement (98.3%) rates when compared to xenodiagnosis and hemoculture. Considering the dilution 1:20 to discriminate reactions from the nonreactive ones, the IFAT is indicated as a diagnostic method, opossums. The IFAT on dry filter paper presented low rates of co-positivity (78.2%), agreement (76.0%) and consistent disagreement related to the non-reactive results.
Fernandes
A J
AJ
Centro de Pesquisas René Rachou/FIOCRUZ, Belo Horizonte, MG.
Chiari
E
E
Chiari
C A
CA
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D014355:Chagas Disease / Q000097:blood / Q000175:diagnosis* / Q000662:veterinary; D005455:Fluorescent Antibody Technique; D009893:Opossums / Q000097:blood / Q000469:parasitology*; D014349:Trypanosoma cruzi / Q000276:immunology
D000913:Antibodies, Protozoan
Diagnóstico de infecção pelo Trypanosoma cruzi em gambas, naturalmente infectados, pela reação de imunofluorescência indireta.