Rapid quantitation of DNA spot hybridization by flatbed scintillation counting.
The flatbed scintillation counting system (Betaplate) was used for quantitative measurement of the radioactive hybridization signal in detection of adenovirus and papillomavirus DNA in clinical specimens. In this method, 96 samples on a nylon membrane can be handled as a single entity throughout the hybridization and counting procedure. The technique is sensitive, rapid, and convenient in routine use when compared with conventionally applied methods for the numerical analysis of hybridization results. The assay principle allows simultaneous testing of large numbers of specimens.
Hyypiä
T
T
Department of Virology, University of Turku, Finland.
Auvinen
E
E
Kovanen
S
S
Ståhlberg
T H
TH
D000257:Adenoviridae Infections / Q000175:diagnosis; D001345:Autoradiography; D015342:DNA Probes; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D005069:Evaluation Studies as Topic; D006801:Humans; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000302:isolation & purification; D012588:Scintillation Counting / Q000379:methods*; D014412:Tumor Virus Infections / Q000175:diagnosis
D015342:DNA Probes; D015344:DNA Probes, HPV; D004279:DNA, Viral