Both granulocyte-macrophage colony-stimulating factor and monocytic colony-stimulating factor are produced by the human T-cell line, HUT 102.
We present the first report of a T-helper cell line, HUT 102, constitutively producing both granulocyte-macrophage colony-stimulating factor (GM-CSF) and monocytic colony-stimulating factor (M-CSF), as detected by sensitive enzyme-linked immunosorbent assays and Northern blot analysis. However, neither amplification nor structural change of the GM-CSF and M-CSF genes was detected by Southern blot analysis. In the case of HUT 102, in which human T-lymphotropic leukemia virus type I (HTLV-I) is integrated, the viral protein, which acts as a trans-acting transcriptional activator, may induce the production of both GM-CSF and M-CSF.
Kawasaki
C
C
First Department of Internal Medicine, Kyushu University, Fukuoka, Japan.
Okamura
S
S
Omori
F
F
Shimoda
K
K
Hayashi
S
S
Kondo
S
S
Yamada
M
M
Niho
Y
Y
D002460:Cell Line; D015342:DNA Probes; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII; D004797:Enzyme-Linked Immunosorbent Assay; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000096:biosynthesis* / Q000235:genetics; D009693:Nucleic Acid Hybridization; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism*
D015342:DNA Probes; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII