Radiolabelling of platelets with technetium-99m.
A method for labelling of platelets with technetium-99m (Tc-99m) is presented. In principle, aminobenzoic acid and tartaric acid are used as reagents, allowing Tc-99m complexes of intermediate chemical stability to be formed. These complexes react rapidly with proteins, such as platelet proteins, when added. We have examined the isolation procedure for the platelets and the labelling procedure using residual aggregational ability and residual content of beta-thromboglobulin (beta-TG) as indicators of damage to the platelets. In its final version the method allowed a 32.6 +/- 2.7% (mean +/- SD) incorporation of Tc-99m into platelets which again showed a 66 +/- 15% residual aggregational ability, tested by 50 mumol/l of ADP, and a 79 +/- 17% residual content of beta-TG releasable by 10 IU/ml of thrombin. In a pilot clinical study involving 28 patients we found labelled autologous platelets useful in detecting lung embolism and deep vein thrombosis.
Sundrehagen
E
E
Department of Nuclear Medicine, Norwegian Radium Hospital, Oslo.
Urdal
P
P
Heggli
D E
DE
Lindegaard
M W
MW
Jacobsen
E
E
D000244:Adenosine Diphosphate / Q000494:pharmacology; D062365:Aminobenzoates; D001792:Blood Platelets; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D010865:Pilot Projects; D010974:Platelet Aggregation / Q000187:drug effects; D011655:Pulmonary Embolism / Q000000981:diagnostic imaging; D011877:Radionuclide Imaging; D013644:Tartrates; D013667:Technetium; D013924:Thrombophlebitis / Q000000981:diagnostic imaging; D001620:beta-Thromboglobulin / Q000032:analysis
D062365:Aminobenzoates; D013644:Tartrates; D001620:beta-Thromboglobulin; D000244:Adenosine Diphosphate; D013667:Technetium; C029768:tartaric acid