[The immunoenzymatic assay (ELISA) in the serodiagnosis of Plasmodium vivax].
ELISA was evaluated for the serodiagnosis of Plasmodium vivax using homologous antigen. This was a crude fraction obtained after detergent (NP-40) lysis of human parasitized red blood cells. Antibodies of the classes IgM, IgG, IgA were determined in a pool of eleven sera from patients with P. vivax malaria. The protein A was introduced as secondary probe to screen P. vivax antibodies in 30 sera of patients harbouring a first episode of P. vivax malaria. There was a correlation of 93% with the parasitological diagnosis and the test resulted specific and reproducible.
Perez
H A
HA
Instituto Venezolano de Investigaciones Científicas, IVIC, Centro de Microbiología y Biología Celular, Caracas, Venezuela.
de la Rosa
M
M
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D016780:Malaria, Vivax / Q000175:diagnosis*; D008297:Male; D010966:Plasmodium vivax / Q000276:immunology*; D012698:Serologic Tests; D013205:Staphylococcal Protein A
D000953:Antigens, Protozoan; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D013205:Staphylococcal Protein A
El inmunoensayo enzimático (ELISA) en el diagnóstico serológico de Plasmodium vivax.