mRNA acetylated at 2'-OH-groups of ribose residues is functionally active in the cell-free translation system from wheat embryos.
Modification (acetylation) of 2'-OH groups of mRNA ribose residues does not result in a loss of their template activity in a cell-free translation system from wheat embryos and in some cases activates inactive mRNAs. Modification of 70-75% of 2'-OH groups makes mRNA stable to the effect of pancreatic ribonuclease.
Ovodov SYu
Institute of Protein Research, Academy of Sciences, Moscow, USSR.
Alakhov YuB
D000089:Acetic Anhydrides; D000107:Acetylation; D000595:Amino Acid Sequence; D009320:Atrial Natriuretic Factor / Q000235:genetics; D001483:Base Sequence; D002116:Calcitonin / Q000235:genetics; D002474:Cell-Free System; D003062:Codon; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008969:Molecular Sequence Data; D011072:Poly U / Q000378:metabolism; D014176:Protein Biosynthesis / Q000502:physiology*; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D012260:Ribonucleases; D012266:Ribose / Q000378:metabolism; D012639:Seeds / Q000378:metabolism; D013329:Structure-Activity Relationship; D014908:Triticum
D000089:Acetic Anhydrides; D003062:Codon; D012333:RNA, Messenger; D011072:Poly U; C031800:acetic anhydride; D012266:Ribose; D009320:Atrial Natriuretic Factor; D002116:Calcitonin; D012260:Ribonucleases; D007930:Leucine