Cell cycle regulation of p34cdc2 kinase activity in Physarum polycephalum.
The regulation of the mitotic histone H1 kinase activity has been analyzed during the naturally synchronous cell cycle of Physarum polycephalum plasmodia. The universal binding property of the p13suc1 Schizosaccharomyces pombe gene product was used to precipitate and assay the cdc2 histone H1 kinase activity. The kinase activity peaks at the beginning of metaphase and its decline, which requires protein synthesis, appears to be an early event during the metaphase process. Microtubular poisons, temperature shifts and DNA synthesis inhibitors were used to perturb cell cycle regulatory pathways and characterize their effects on cdc2 kinase activation. Our results suggest that the full activation of the mitotic kinase requires at least two successive triggering signals involving microtubular components and DNA synthesis.
Ducommun
B
B
Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, NY 11724.
Tollon
Y
Y
Garès
M
M
Beach
D
D
Wright
M
M
D016590:Aphidicolin; D001562:Benzimidazoles / Q000494:pharmacology; D016203:CDC2 Protein Kinase / Q000302:isolation & purification / Q000378:metabolism*; D002219:Carbamates; D002453:Cell Cycle / Q000187:drug effects; D018797:Cell Cycle Proteins; D002846:Chromatography, Affinity; D003513:Cycloheximide / Q000494:pharmacology; D004271:DNA, Fungal / Q000096:biosynthesis; D004259:DNA-Directed DNA Polymerase / Q000187:drug effects; D004224:Diterpenes / Q000494:pharmacology; D005656:Fungal Proteins; D006118:Griseofulvin / Q000494:pharmacology; D066298:In Vitro Techniques; D008870:Microtubules / Q000187:drug effects / Q000502:physiology; D010766:Phosphorylation; D010804:Physarum; D029702:Schizosaccharomyces pombe Proteins; D015398:Signal Transduction
D001562:Benzimidazoles; D002219:Carbamates; D018797:Cell Cycle Proteins; D004271:DNA, Fungal; D004224:Diterpenes; D005656:Fungal Proteins; D029702:Schizosaccharomyces pombe Proteins; C051655:Suc1 protein, S pombe; D006118:Griseofulvin; D016590:Aphidicolin; D003513:Cycloheximide; D016203:CDC2 Protein Kinase; D004259:DNA-Directed DNA Polymerase; C006698:carbendazim