In vitro models of B lymphocyte development.
B lymphocyte differentiation proceeds in the bone marrow in association with a supporting framework of stromal cells. The various long-term bone marrow cultures have provided valuable in vitro models for the study of both normal and dysregulated, stromal cell dependent B lymphopoiesis. Stromal cell lines have been cloned from the adherent layers of the cultures, and recent studies indicate these cells produce soluble factors that control the growth and differentiation of B lineage cells. Modifications of the long-term cultures are now being developed that permit specific stages in the B cell developmental pathway to be amplified. These systems should prove valuable for analysis of stromal cell derived signals that regulate the growth and differentiation of B lineage cells.
Henderson
A J
AJ
Division of Biomedical Sciences, University of California, Riverside 92521-0121.
Dorshkind
K
K
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000187:drug effects; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D002117:Calcitriol / Q000494:pharmacology; D002454:Cell Differentiation; D002478:Cells, Cultured; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology; D051379:Mice; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D007375:Interleukin-1; D016212:Transforming Growth Factor beta; D002117:Calcitriol