Selective medium for isolation of Bacteroides gracilis.
A new medium selective for Bacteroides gracilis was developed. The medium is tryptic soy agar (Difco Laboratories, Detroit, Mich.) containing nalidixic acid, teicoplanin, sodium formate, sodium fumarate, and potassium nitrate. All 18 strains of B. gracilis tested grew with only minimal inhibition. Most of the other 214 organisms tested, including most Bacteroides species, other anaerobes, and a substantial number of facultative anaerobes, were significantly inhibited by the medium. In a diagnostic study of 49 clinical specimens (28 patients with intra-abdominal infection, mostly gangrenous or perforated appendicitis), four strains of B. gracilis were isolated (from 4 different patients) on B. gracilis selective agar but were not detected on standard media.
Lee
K
K
Department of Clinical Pathology, Wonju Medical College, Yonsei University, Korea.
Baron
E J
EJ
Summanen
P
P
Finegold
S M
SM
D000362:Agar; D000900:Anti-Bacterial Agents; D001064:Appendicitis / Q000150:complications / Q000175:diagnosis / Q000473:pathology; D001439:Bacteroides / Q000302:isolation & purification*; D001442:Bacteroides Infections / Q000150:complications / Q000175:diagnosis / Q000473:pathology; D002364:Caseins; D003470:Culture Media; D005561:Formates; D005650:Fumarates; D005734:Gangrene; D006020:Glycopeptides; D006801:Humans; D009268:Nalidixic Acid; D009566:Nitrates; D017680:Potassium Compounds; D011492:Protein Hydrolysates; D012463:Saliva / Q000382:microbiology*; D017334:Teicoplanin
D000900:Anti-Bacterial Agents; D002364:Caseins; D003470:Culture Media; D005561:Formates; D005650:Fumarates; D006020:Glycopeptides; D009566:Nitrates; D017680:Potassium Compounds; D011492:Protein Hydrolysates; C026375:trypticase-soy broth; D009268:Nalidixic Acid; D017334:Teicoplanin; D000362:Agar; C023844:potassium nitrate