Two-dimensional chromatographic method for determination of aflatoxins in liver; occurrence of aflatoxins in animal liver.
A thin-layer chromatographic method is described for the analysis of aflatoxins in animal liver. Liver samples are extracted with chloroform and phosphoric acid. After filtration, an aliquot is evaporated and defatted by liquid-liquid partitioning. The extract is submitted to silica gel minicolumn cleanup and the final extract is concentrated and submitted to two-dimensional thin-layer chromatography (TLC). The identity of aflatoxins B1 and M1 is confirmed with trifluoroacetic acid (TFA) carried out on the thin-layer plate used for quantitation of these aflatoxins. The method permits the detection and confirmation of aflatoxins in liver in concentrations as low as 0.05 micrograms/kg. Average recoveries for aflatoxin M1 and aflatoxin B1 at spiking levels of 0.2 micrograms/kg were found to be 65% and 85%, respectively. With this method, 73 samples of bovine liver, 70 samples of porcine liver, and 56 samples of chicken liver taken from different slaughterhouses were investigated. In one sample of bovine liver, aflatoxins B1, B2, and M1 could be detected in concentrations of 0.10, 0.03, and 0.08 micrograms/kg, respectively.
Van Egmond
H P
HP
National Institute of Public Health and Environmental Protection, Laboratory for Residue Analysis, Bilthoven, The Netherlands.
Paulsch
W E
WE
Sizoo
E A
EA
Schuller
P L
PL
D016604:Aflatoxin B1; D016607:Aflatoxin M1; D000348:Aflatoxins / Q000032:analysis*; D000818:Animals; D002855:Chromatography, Thin Layer / Q000379:methods; D007202:Indicators and Reagents; D008099:Liver / Q000737:chemistry*; D012997:Solvents; D014269:Trifluoroacetic Acid
D000348:Aflatoxins; D007202:Indicators and Reagents; D012997:Solvents; D016607:Aflatoxin M1; D016604:Aflatoxin B1; D014269:Trifluoroacetic Acid