Regulation of the cytosolic free calcium concentration by Na+ spikes in immature cerebellar neurons with N-methyl-D-aspartate receptors.
N-Methyl-D-aspartate and glycine increased the cytosolic free calcium concentration ([Ca]in) in medium-sized cerebellar neurons. Spontaneous changes in [Ca]in were occasionally observed in NMDA-responsive cells, but large increases in [Ca]in were triggered only through depolarizations by adding veratridine or K+ channel blockers in every cell examined. The [Ca]in increase was suppressed by voltage-dependent Na+ and Ca2+ channel blockers and by an inhibitory transmitter (GABA), suggesting that the generation of Na+ spikes is involved in the increase in [Ca]in.
Sorimachi
M
M
Department of Physiology, Kagoshima University, School of Medicine, Japan.
Morita
Y
Y
Kuramoto
K
K
D000200:Action Potentials / Q000187:drug effects; D000375:Aging / Q000502:physiology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects*; D003600:Cytosol / Q000378:metabolism*; D005711:Gallopamil / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D016194:Receptors, N-Methyl-D-Aspartate / Q000187:drug effects*; D012964:Sodium / Q000502:physiology; D013779:Tetrodotoxin / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D016194:Receptors, N-Methyl-D-Aspartate; D005711:Gallopamil; D013779:Tetrodotoxin; D005680:gamma-Aminobutyric Acid; D012964:Sodium; D002118:Calcium