Production and characterisation of monoclonal antibodies for species diagnosis of sarcosporidia.
Monoclonal antibodies were raised against cystozoites of Sarcocystis muris and characterised. Twelve monoclonal antibodies reacted in the ELISA, Dot-ELISA and IFAT only with homologous antigen. The other twelve showed cross reactions of various degrees with cystozoites of S. gigantea, S. tenella, S. arieticanis, S. capracanis, S. miescheriana and S. suihominis. Proteins of S. arieticanis, S. tenella, S. gigantea, S. capracanis, S. muris and Toxoplasma gondii were compared by isoelectric focusing and showed pronounced differences.
Rommel
M
M
Institute for Parasitology, Hanover Veterinary School, Federal Republic of Germany.
Tenter
A M
AM
Vietmeyer
C
C
Mencke
N
N
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D007525:Isoelectric Focusing; D012522:Sarcocystis / Q000276:immunology / Q000302:isolation & purification*; D013045:Species Specificity
D000911:Antibodies, Monoclonal