A novel method for isolation and concentration of ribonuclease T1 from Taka-Diastase.
A novel method for isolation and concentration of RNase T1 from Taka-Diastase is developed. It is a combination method of bentonite adsorption with dialysis desorption. In the present method, RNase T1 can be concentrated about ten-fold, the recovery of total activity was greater than 95%, and specific activity was raised 8-10 folds. Further purification with ammonium sulfate precipitation and chromatography on DEAE-cellulose and DEAE-Sephadex yields a RNase T1 which contains no pMase. pDase nor RNase T2 activities and a 750 fold increase in specific activity. Our method is more simple, rapid, and efficient than previous methods.
Zhang
Q J
QJ
Institute of Biophysics, Chinese Academy of Sciences, Beijing.
Liu
Z Y
ZY
D000645:Ammonium Sulfate; D001546:Bentonite / Q000494:pharmacology; D011232:Chemical Precipitation; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003956:Dialysis; D006163:Ribonuclease T1 / Q000302:isolation & purification* / Q000378:metabolism; D000516:alpha-Amylases / Q000302:isolation & purification*
D001546:Bentonite; D006163:Ribonuclease T1; D000516:alpha-Amylases; D000645:Ammonium Sulfate