Immunohistological study on the distribution of alpha 1-antichymotrypsin in Alzheimer's brain, compared to beta-amyloid precursor protein and beta-amyloid protein.
Distribution of alpha 1-antichymotrypsin was immunohistologically examined in Alzheimer's brains and compared with those of beta-amyloid precursor protein and beta-amyloid protein. Immunoblotting study revealed that alpha 1-antichymotrypsin existed in senile plaques extracted from Alzheimer's brain. Immunohistologically, rabbit polyclonal antiserum to alpha 1-antichymotrypsin reacted not only with well-defined amyloid deposits such as typical, primitive, core plaques and cerebrovascular amyloid, but also with so-called preamyloid deposits including large amorphous materials (diffuse plaques), subpial amorphous materials and granular deposits. The staining pattern of alpha 1-antichymotrypsin was almost the same as those of beta-amyloid precursor protein and beta-amyloid protein. The present study indicated that alpha 1-antichymotrypsin could play an important role in the formation of various kinds of amyloid deposits including preamyloid ones in Alzheimer's brain.
Takahashi
H
H
Department of Neurology, School of Medicine, Tokyo Medical and Dental University, Japan.
Hirokawa
K
K
Tsukagoshi
H
H
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000378:metabolism / Q000473:pathology*; D016229:Amyloid beta-Peptides / Q000032:analysis*; D016564:Amyloid beta-Protein Precursor; D001923:Brain Chemistry; D006801:Humans; D008875:Middle Aged; D011498:Protein Precursors / Q000032:analysis*; D000514:alpha 1-Antichymotrypsin / Q000032:analysis*
D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D011498:Protein Precursors; D000514:alpha 1-Antichymotrypsin