Biological and immunological properties of Sugi basic protein-pullulan conjugate. II. Is the reduced ability to elicit the Arthus reaction based on the poor activation of complement by immune complex consisting of anti-Sugi basic protein and Sugi basic protein-pullulan?
Ability of Sugi basic protein (SBP)-pullulan conjugate to elicit the Arthus reaction was found to be markedly reduced, about 100 times lower than that of native SBP. To analyze this reduced ability, activation of complement by immune complex consisting of SBP-pullulan and anti-SBP antibodies was studied. Tests for complement consumption, C3 conversion and cleavage of factor B revealed that immune complex formed with SBP-pullulan is incapable of supporting efficient activation of the complement system. Previously, we have shown data suggesting that SBP-pullulan conjugate would be a good candidate for desensitization therapy against cedar pollinosis. The results presented in this paper provide additional support for the suggestion.
Usui
M
M
Hayashibara Biochemical Laboratories, Inc., Okayama, Japan.
Taniguchi
Y
Y
Ando
S
S
Kurimoto
M
M
Matuhasi
T
T
D000485:Allergens / Q000008:administration & dosage / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D000936:Antigen-Antibody Complex / Q000276:immunology* / Q000494:pharmacology; D052179:Antigens, Plant; D001183:Arthus Reaction / Q000209:etiology / Q000276:immunology*; D003167:Complement Activation; D003176:Complement C3 / Q000378:metabolism; D003170:Complement Pathway, Alternative; D003171:Complement Pathway, Classical; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D005936:Glucans / Q000008:administration & dosage / Q000276:immunology*; D006168:Guinea Pigs; D006801:Humans; D010940:Plant Proteins; D011817:Rabbits
D000485:Allergens; D000906:Antibodies; D000936:Antigen-Antibody Complex; D052179:Antigens, Plant; D003176:Complement C3; C065290:Cry j I protein, Cryptomeria japonica; D005936:Glucans; D010940:Plant Proteins; C009109:pullulan