Recovery of different stages of Dictyocaulus viviparus from cattle lungs by a combination of a perfusion and a Baermann technique.
The lungs of 71 calves used in two grazing studies were examined for lungworms with a perfusion method combined with a modified Baermann method. The numbers of worms recovered from the lungs were 11,630 in total, 7495 adults, 872 juvenile fifth stage larvae (L5) and 3263 inhibited L5. The percentages found after perfusion were 78.9 per cent in total, 91.8 per cent adults, 78.6 per cent juvenile L5 and 49.6 per cent inhibited L5. The perfusion method seemed adequate and rapid for recovering the adult and juvenile stages but not the inhibited stage. For estimating the numbers of inhibited larvae a combination of perfusion with the Baermann technique is necessary.
Eysker
M
M
Institute of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
Boersema
J H
JH
Hendrikx
W M
WM
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology*; D004021:Dictyocaulus / Q000302:isolation & purification*; D004022:Dictyocaulus Infections / Q000469:parasitology*; D008168:Lung / Q000469:parasitology*; D008174:Lung Diseases, Parasitic / Q000469:parasitology / Q000662:veterinary; D010477:Perfusion