Interleukin 3 induces histamine synthesis in the human hemopoietic system.
Recombinant human interleukin 3 (rhIL-3) induces an increase in histamine production by human bone marrow, fetal liver, and cord blood cells. This phenomenon, already significant after 3 days of incubation, is strikingly enhanced following either enrichment in immature cell subpopulations or CD8+ cell depletion. It results from an increase in histamine synthesis because of 1) the low level of histamine cell content before any incubation, 2) the parallel increase in both extra- and intracellular histamine levels in response to rhIL-3, and 3) the early IL-3-induced increase in L-histidine decarboxylase (HDC; EC 4.1.1.22) activity. Moreover, rhIL-3 has no similar activity on adult peripheral blood cells, suggesting that it might be specific to the hemopoietic system.
Minkowski
M
M
CNRS URA 122, INSERM U 25, Necker Hospital, Paris, France.
Lebel
B
B
Arnould
A
A
Dy
M
M
D000328:Adult; D001853:Bone Marrow / Q000201:enzymology; D001854:Bone Marrow Cells; D002455:Cell Division; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D005312:Fetal Blood / Q000166:cytology / Q000201:enzymology; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006632:Histamine / Q000096:biosynthesis*; D006640:Histidine Decarboxylase / Q000378:metabolism; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000196:embryology; D050378:T-Lymphocytes, Regulatory / Q000473:pathology
D007377:Interleukin-3; D006632:Histamine; D006640:Histidine Decarboxylase