Evidence for an endogenous ATPase inhibitor protein in plant mitochondria. Purification and characterization.
An endogenous ATPase inhibitor protein has been identified and isolated for the first time from plant mitochondria. The inhibitor protein was isolated from potato (Solanum tuberosum) tuber mitochondria and purified to homogeneity. The isolated inhibitor is a heat-stable, trypsin-sensitive, basic protein, with a molecular mass approximately 8.3 kDa. Amino acid analysis reveals a high content of glutamic acid, lysine and arginine and the absence of proline; threonine and leucine. The interaction of the inhibitor with F1-ATPase requires the presence of Mg2(+)-ATP in the incubation medium. The ATPase activity of isolated F1 is inhibited to 50% in the presence of 14 micrograms inhibitor/mg F1. A stoichiometry of 1.3 mol inhibitor/mol F1 for complete inhibition can be calculated from this value. The potato ATPase inhibitor is also a potent inhibitor of the ATPase activity of the isolated yeast F1. The inhibitor resembles the ATPase inhibitors of yeast and mammalian mitochondria, and does not seem to be related to the inhibitory peptide, epsilon subunit, of chloroplast ATPase.
Norling
B
B
Department of Biochemistry, Arrhenius Laboratories for Natural Sciences, University of Stockholm, Sweden.
Tourikas
C
C
Hamasur
B
B
Glaser
E
E
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000596:Amino Acids / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D008274:Magnesium / Q000494:pharmacology; D008928:Mitochondria / Q000032:analysis*; D008970:Molecular Weight; D010940:Plant Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D011506:Proteins / Q000302:isolation & purification*; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D011198:Solanum tuberosum / Q000032:analysis* / Q000201:enzymology; D014357:Trypsin; D000097795:ATPase Inhibitory Protein
D000255:Adenosine Triphosphate; D000596:Amino Acids; D008274:Magnesium; D010940:Plant Proteins; D011506:Proteins; D006180:Proton-Translocating ATPases; D014357:Trypsin; D000097795:ATPase Inhibitory Protein