Mitogenic induction of ornithine decarboxylase in human mononuclear leukocytes: relationships with adenosine diphosphate ribosyltransferase.
1. Relationships between ornithine decarboxylase (ODC) and adenosine diphosphate ribosyl transferase (ADPRT) in human mononuclear leukocytes (HML) were tested by statistical comparisons of their values in a group of 46 people, and by use of inhibitors of ADPRT. 2. ODC was assayed following exposure of HML, for 20 hr, to mitogens [phytohemagglutinin (PHA) and pokeweed mitogen]; ADPRT was measured following exposure of HML to H2O2 (100 microM) for 1 hr (activated ADPRT), and in parallel cultures without H2O2 (constitutive ADPRT). 3. Significant correlations were found between ODC and ADPRT values; the effects of smoking disturbed the correlations. PHA induction of ODC was negatively influenced by age (standardized beta coefficient = -2.95, P = 0.005), while age also influenced ADPRT values negatively in non-smokers (for H2O2 activated ADPRT, standardized beta coefficient = -2.74, P less than 0.008). 4. Inhibitors of ADPRT, nicotinamide, caffeine and benzamide inhibited the induction of ODC by PHA in a concentration-dependent manner, in the range (0.6-10 mM) known to inhibit ADPRT.
Johnson
D B
DB
Preventive Medicine Institute/Strange Clinic, New York, NY 10016.
Markowitz
M M
MM
Joseph
P E
PE
Miller
D G
DG
Pero
R W
RW
D000367:Age Factors; D001549:Benzamides / Q000494:pharmacology; D015415:Biomarkers; D002110:Caffeine / Q000494:pharmacology; D002478:Cells, Cultured; D004790:Enzyme Induction; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000201:enzymology*; D015999:Multivariate Analysis; D009536:Niacinamide / Q000494:pharmacology; D009955:Ornithine Decarboxylase / Q000096:biosynthesis* / Q000097:blood; D010835:Phytohemagglutinins / Q000494:pharmacology*; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*
D001549:Benzamides; D015415:Biomarkers; D010835:Phytohemagglutinins; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; C047876:phytohemagglutinin-M; D009536:Niacinamide; D002110:Caffeine; D011065:Poly(ADP-ribose) Polymerases; D009955:Ornithine Decarboxylase