Dopamine D2 receptors and spinal cord excitation in mice.
Spinal cord excitation was induced in mice by morphine and the effects of dopamine D1 and D2 receptor antagonists on the Straub tail reaction were investigated. The dopamine D2 receptor antagonist, sulpiride (25-100 mg/kg i.p.), or haloperidol (0.25-1.0 mg/kg dose dependently inhibited the Straub tail reaction induced by subcutaneously injected morphine. A low dose of apomorphine (50 micrograms/kg s.c.) also reduced the Straub tail reaction. The dopamine D1 receptor antagonist, SCH-23390 (25-100 micrograms/kg i.p.), had no significant effect. Sulpiride (50 mg/kg i.p.) significantly inhibited the Straub tail reaction induced by intrathecally injected morphine (6 microgram/mouse). Intrathecal injection of apomorphine (12.5-25 micrograms/mouse) induced the Straub tail reaction dose dependently. The Straub tail reaction induced by intrathecally injected apomorphine was significantly inhibited by sulpiride. SCH-23390 had no significant effect on the Straub tail reaction induced by intrathecally injected morphine or apomorphine. These results support the proposal that the dopamine response involved in the Straub tail reaction is mediated by postsynaptic dopamine D2 receptors in the spinal cord of mice.
Hasegawa
Y
Y
Department of Pharmacology, Research Center, Taisho Pharmaceutical Co., Ltd., Saitama, Japan.
Kurachi
M
M
Otomo
S
S
D000818:Animals; D001058:Apomorphine / Q000037:antagonists & inhibitors; D001552:Benzazepines / Q000494:pharmacology; D018492:Dopamine Antagonists; D006220:Haloperidol / Q000494:pharmacology; D007278:Injections, Spinal; D007279:Injections, Subcutaneous; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009020:Morphine / Q000037:antagonists & inhibitors; D011954:Receptors, Dopamine / Q000502:physiology; D017448:Receptors, Dopamine D2; D012018:Reflex / Q000187:drug effects / Q000502:physiology; D013116:Spinal Cord / Q000187:drug effects* / Q000502:physiology; D013469:Sulpiride / Q000494:pharmacology
D001552:Benzazepines; D018492:Dopamine Antagonists; D011954:Receptors, Dopamine; D017448:Receptors, Dopamine D2; D009020:Morphine; D013469:Sulpiride; D006220:Haloperidol; D001058:Apomorphine