Characterization of suppressor T cells for antibody production by chicken spleen cells. II. Comparison of CT8+ cells from concanavalin A-injected normal and bursa cell-injected agammaglobulinaemic chickens.
The phenotypes of two different types of suppressor T cells in the chicken, both capable of inhibiting secondary antibody responses in vitro, were determined. The first of these, induced by injection of concanavalin A (Con A) into normal chickens, was CT8+, TcR2+ (alpha beta), CT4-, TcR1- (gamma delta). These cells appeared to exhibit histamine type 2 (H2) receptors, as they adhered to cimetidine-BSA-coated dishes. Moreover, cimetidine added to the medium at 2 x 10(-4) M completely prevented the suppression induced by these suppressor cells. The second type of 'suppressor' T-cell studied, induced in agammaglobulinaemic (A gamma) chickens by injection of bursa cells, exhibited the same phenotype, but was insensitive to cimetidine and did not adhere to cimetidine-BSA-coated dishes, indicating heterogeneity with respect to H2 receptor expression on CT8+ chicken T cells with suppressor activity. The results also showed that a relatively larger proportion of CT8+ than of CT4+ cells adhered to cimetidine-BSA-coated dishes and thus appeared to be H2 receptor positive. TcR1 (gamma delta) cells did not contribute significantly to the antigen non-specific suppressor effects examined in this study.
Quere
P
P
Station de Pathologie Aviaire, Institut National de la Recherche Agronomique, Monnaie, France.
Bhogal
B S
BS
Thorbecke
G J
GJ
D000361:Agammaglobulinemia / Q000276:immunology*; D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D002060:Bursa of Fabricius / Q000276:immunology; D002645:Chickens; D002927:Cimetidine / Q000378:metabolism; D003208:Concanavalin A / Q000276:immunology; D006462:Hemolytic Plaque Technique; D007156:Immunologic Memory; D011970:Receptors, Histamine H2 / Q000032:analysis; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013154:Spleen / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte; D011970:Receptors, Histamine H2; D003208:Concanavalin A; D012710:Serum Albumin, Bovine; D002927:Cimetidine