A specific defect in CD3 gamma-chain gene transcription results in loss of T-cell receptor/CD3 expression late after human immunodeficiency virus infection of a CD4+ T-cell line.
Sequential effects on cellular protein expression following human immunodeficiency virus (type 1) infection of a CD4+ T-cell line in vitro were investigated. Events in the human interleukin 2-dependent helper T-cell line WE17/10 are similar in several respects to the clinical progression in acquired immunodeficiency syndrome. WE17/10 cell infection is characterized by an extended period during which viral replication occurs without accompanying cytotoxicity and with a maximum 30% decrease in surface CD4. Cellular protein expression generally remains unaffected during this first phase of infection. However, after 2-3 months, a severe defect in the expression of the T-cell receptor/CD3 complex both on the cell surface and inside the cell becomes apparent. Other cell membrane markers, such as CD2 and CD25, remain constant throughout the course of infection; after its initial decrease, CD4 remains at 70% of control values. Lack of surface expression of the TCR/CD3 complex is correlated with a specific defect in transcription of the CD3 gamma-chain gene.
Willard-Gallo
K E
KE
International Institute of Cellular and Molecular Pathology, Brussels, Belgium.
Van de Keere
F
F
Kettmann
R
R
D015703:Antigens, CD / Q000032:analysis / Q000235:genetics*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics* / Q000302:isolation & purification; D017252:CD3 Complex; D015704:CD4 Antigens / Q000276:immunology*; D002460:Cell Line; D002472:Cell Transformation, Viral; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D046911:Macromolecular Substances; D012333:RNA, Messenger / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000235:genetics* / Q000302:isolation & purification; D013601:T-Lymphocytes / Q000276:immunology*; D014158:Transcription, Genetic
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D015704:CD4 Antigens; D046911:Macromolecular Substances; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell