title: Isolation and expression of cDNA for different forms of hepatocyte growth factor from human leukocyte.
abstract: Human leukocyte cDNA library was screened to isolate cDNA clones coding for hepatocyte growth factor using cDNA from human liver as a probe. Nucleotide and deduced amino acid sequences were analyzed for two of four clones obtained. One of them contained an open reading frame coding for a polypeptide chain of 728 amino acid residues like that of cDNA clone derived from human liver. In another clone a spontaneous deletion of 15 base pairs was found within the coding sequence. When expressed transiently using COS-1 cells both clones produced protein with similar biological activity against rat hepatocyte in vitro.
authors: Pharmaceutical Research Center, Toyobo Co. Ltd., Otsu, Japan.
authors: T
authors: T
authors: Seki
authors: I
authors: I
authors: Ihara
authors: A
authors: A
authors: Sugimura
authors: M
authors: M
authors: Shimonishi
authors: T
authors: T
authors: Nishizawa
authors: O
authors: O
authors: Asami
authors: M
authors: M
authors: Hagiya
authors: T
authors: T
authors: Nakamura
authors: S
authors: S
authors: Shimizu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002455:Cell Division; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000097:blood / Q000235:genetics* / Q000302:isolation & purification; D015723:Gene Library; D006133:Growth Substances / Q000097:blood / Q000235:genetics*; D017228:Hepatocyte Growth Factor; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D015183:Restriction Mapping; D014162:Transfection
chemical_list: D006133:Growth Substances; D015345:Oligonucleotide Probes; D017228:Hepatocyte Growth Factor; D004247:DNA