Glucose content and efficiency of glycolysis in protected ischemic kidneys of different species.
In ischemic canine kidneys protected by Bretschneider's HTK solution the glycolytic lactate production is limited by a low renal substrate content. However, for anaerobic energy supply ischemic organs depend on glycolysis. To evaluate the role of glycolysis in renal protection, the relationship between lactate production and anaerobic energy supply was examined in protected kidneys of dogs, sheep, and swine. Additionally, in canine kidneys an attempt was made to improve anaerobic energy provision by adding glucose to the protective solution. The results were as follows: (1) According to increasing lactate production from swine to dog to sheep, intraischemic ATP decay was delayed least in swine and most in sheep. (2) Glucose addition (10 mM) to the HTK solution roughly doubled the time for ATP to fall to 1 mumol/g dry wt (tATP) in dogs. (3) The greater the lactate production in all three species, the lower the decrease in SAN (ATP + ADP + AMP) from 5 to 120 min of ischemia. (4) A glucose additive in the protective solution led to a significant (p less than .005) increase of SAN in dogs at 120 min of ischemia. A sufficient substrate supply seems to be an essential component of a reliable renal protection.
Kehrer
G
G
Department of Physiology and Pathophysiology, University of Göttingen, FRG.
Blech
M
M
Kallerhoff
M
M
Kleinert
H
H
Langheinrich
M
M
Bretschneider
H J
HJ
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D004285:Dogs / Q000378:metabolism*; D004734:Energy Metabolism / Q000187:drug effects*; D005947:Glucose / Q000032:analysis* / Q000494:pharmacology; D006019:Glycolysis; D007511:Ischemia / Q000378:metabolism*; D007668:Kidney / Q000098:blood supply* / Q000378:metabolism; D007773:Lactates / Q000096:biosynthesis; D019344:Lactic Acid; D008353:Mannitol / Q000494:pharmacology; D009926:Organ Preservation; D011189:Potassium Chloride / Q000494:pharmacology; D011343:Procaine / Q000494:pharmacology; D012756:Sheep / Q000378:metabolism*; D013045:Species Specificity; D013552:Swine / Q000378:metabolism*
D000227:Adenine Nucleotides; C039984:Bretschneider cardioplegic solution; D007773:Lactates; D019344:Lactic Acid; D008353:Mannitol; D011343:Procaine; D011189:Potassium Chloride; D005947:Glucose