title: Binary affinity chromatography for the purification of glycogen phosphorylase.
abstract: A binary affinity chromatography medium was prepared and found to be useful for the purification and quantitative isolation of glycogen phosphorylase from rabbit skeletal muscle and liver. Glycogen is used as the binary ligand as it has affinity toward both the column matrix and the enzyme. Agarose beads derivatized with concanavalin A bound glycogen to the level of 35 mg/ml. The glycogen-impregnated beads were able to bind 9 mg/ml of phosphorylase a or b. The phosphorylase is tightly bound so that the column can be washed free of contaminants before quantitative elution of the phosphorylase by 2 M glucose, which releases the glycogen-phosphorylase complex. It appears that binary affinity chromatography may have general utility for the isolation and purification of enzymes and other specific binding agents.
authors: Department of Biochemistry, University of Minnesota Medical School, Minneapolis 55455.
authors: M E
authors: ME
authors: Gurnack
authors: R D
authors: RD
authors: Edstrom
mesh_terms: D000818:Animals; D001665:Binding Sites; D002846:Chromatography, Affinity / Q000379:methods*; D006003:Glycogen; D008099:Liver / Q000201:enzymology; D009132:Muscles / Q000201:enzymology; D010762:Phosphorylase a / Q000302:isolation & purification / Q000378:metabolism; D010763:Phosphorylase b / Q000302:isolation & purification / Q000378:metabolism; D006005:Phosphorylases / Q000302:isolation & purification* / Q000378:metabolism; D011817:Rabbits
chemical_list: D006003:Glycogen; D010762:Phosphorylase a; D010763:Phosphorylase b; D006005:Phosphorylases