Specific binding of human interleukin-3 and granulocyte-macrophage colony-stimulating factor to human basophils.
The human T cell-derived cytokines interleukin (IL)-3 and granulocyte-macrophage colony-stimulating factor (GM-CSF) were examined for their ability to bind to human basophils. Basophils were obtained from the peripheral blood of a patient with chronic myeloid leukemia undergoing basophilic differentiation after purification on a density gradient of metrizamide. Binding studies with 125I-labeled IL-3 and 125I-labeled GM-CSF demonstrated that basophils express a single class of high-affinity receptors for each of these molecules. Saturation binding curves with 125I-labeled IL-3 revealed that IL-3 bound specifically to basophils, and analysis according to the method of Scatchard revealed that basophils express 800 to 900 receptors per cell with an apparent dissociation constant of 2.6 x 10(-11) mol/L. Saturation-binding curves with 125I-labeled GM-CSF revealed that basophils express 100 to 200 receptors per cell with an apparent dissociation constant of 4 x 10(-11) mol/L. The demonstration of high-affinity receptors for IL-3 and GM-CSF on human basophils suggests a role for these cytokines in the regulation of basophil function.
Lopez
A F
AF
Division of Human Immunology, Institute of Medical and Veterinary Science, Adelaide, South Australia.
Lyons
A B
AB
Eglinton
J M
JM
Park
L S
LS
To
L B
LB
Clark
S C
SC
Vadas
M A
MA
D001491:Basophils / Q000378:metabolism*; D003115:Colony-Stimulating Factors / Q000032:analysis / Q000378:metabolism*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D007377:Interleukin-3 / Q000032:analysis / Q000378:metabolism*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood; D011485:Protein Binding / Q000502:physiology; D011869:Radioligand Assay; D011956:Receptors, Cell Surface / Q000032:analysis / Q000378:metabolism; D016188:Receptors, Granulocyte Colony-Stimulating Factor; D011971:Receptors, Immunologic / Q000032:analysis / Q000378:metabolism; D016185:Receptors, Interleukin-3; D011994:Recombinant Proteins / Q000032:analysis / Q000378:metabolism
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007377:Interleukin-3; D011956:Receptors, Cell Surface; D016188:Receptors, Granulocyte Colony-Stimulating Factor; D011971:Receptors, Immunologic; D016185:Receptors, Interleukin-3; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor