MHC class I antigen expression and alterations induced by rIFN gamma in tumor hybrid cell immunogenicity.
Hybridization of a poorly immunogenic tumor cell with an allogeneic cell was performed in order to improve tumor immune response; several variants derived from one hybrid tumor cell were studied. We compared the immunogenicity of these variants and their allogeneic and syngeneic class I antigen expression before and after IFN gamma treatment. Allogeneic class I antigens were weakly expressed in all variants; IFN treatment enhanced their expression similarly in both immunogenic and nonimmunogenic variants. Syngeneic class I antigen expression differed among variants: IFN treatment induced changes in their expression which corresponded to a posttranscriptional event and which could, at least partly, explain the modifications observed in their immunogenicity.
Goguel
A F
AF
Laboratoire d'Immunobiologie, Institut de Recherches Scientifiques sur le Cancer, Villejuif, France.
Le Borgne
F
F
Baude
B
B
Canavate
M L
ML
Tlouzeau
S
S
Derhy
M L
ML
Lespinats
G
G
D000818:Animals; D000951:Antigens, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D005354:Fibrosarcoma / Q000276:immunology / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006183:H-2 Antigens / Q000096:biosynthesis* / Q000235:genetics; D064532:Histocompatibility Antigen H-2D; D006822:Hybrid Cells / Q000187:drug effects* / Q000276:immunology; D007371:Interferon-gamma / Q000494:pharmacology*; D007739:L Cells / Q000187:drug effects* / Q000276:immunology; D008297:Male; D051379:Mice; D011994:Recombinant Proteins; D013268:Stimulation, Chemical; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000276:immunology
D000951:Antigens, Neoplasm; D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D064532:Histocompatibility Antigen H-2D; D011994:Recombinant Proteins; D007371:Interferon-gamma