Expression of c-fos protein by N-methyl-D-aspartic acid in hypothalamus of immature female rats: blockade by MK-801 or neonatal treatment with monosodium glutamate.
Significant NMDA-induced cellular activity in the median eminence-arcuate region of immature female rats was immunocytochemically localized via c-fos protein-like immunoreactivity. The production of this protein was completely prevented by pre-injection of MK-801 or APV, antagonists of the NMDA receptor-coupled ion channel. Treatment of newborn rats with monosodium glutamate (MSG), which destroys glutamate-sensitive neurons, significantly attenuated NMDA-induced c-fos when tested in the peripubertal period. These results implicate NMDA receptors in the expression of c-fos protein-like immunoreactivity in cells of the arcuate nucleus-median eminence of immature female rats.
MacDonald
M C
MC
Department of Obstetrics and Gynecology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada.
Robertson
H A
HA
Wilkinson
M
M
D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D001111:Arcuate Nucleus of Hypothalamus / Q000254:growth & development; D016291:Dizocilpine Maleate / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D007031:Hypothalamus / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology; D008473:Median Eminence / Q000254:growth & development; D016202:N-Methylaspartate / Q000494:pharmacology*; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000235:genetics*; D016760:Proto-Oncogene Proteins c-fos; D051381:Rats; D012016:Reference Values; D012970:Sodium Glutamate / Q000494:pharmacology*
D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D016202:N-Methylaspartate; D016291:Dizocilpine Maleate; D011505:Protein-Tyrosine Kinases; D012970:Sodium Glutamate