Fluorescence polarization immunoassay of urinary vanillylmandelic acid.
This rapid fluorescence polarization immunoassay for urinary vanillylmandelic acid (VMA) involves use of our previously described antiserum and label and the program for 5-hydroxyindoleacetic acid in the Abbott TDx fluorimeter. Urine samples were measured directly, without pretreatment. The minimum detectable concentration was 0.3 mg/L, and the range of the standard curve was 0.3-200.0 mg/L. Precision, analytical recovery, and correlation of results with those by the Pisano method (Clin Chim Acta 1962;7:285-91) were all satisfactory. With this procedure one can determine the VMA concentration in 10 urine sample in 22 min. This is the first report of a clinical immunoassay for VMA and should greatly simplify screening for neural crest tumors.
Mellor
G W
GW
Department of Chemical Pathology, St. Bartholomew's Hospital, West Smithfield, London, U.K.
Gallacher
G
G
D005454:Fluorescence Polarization; D006801:Humans; D007118:Immunoassay; D008403:Mass Screening; D009373:Neoplasms, Germ Cell and Embryonal / Q000652:urine; D009432:Neural Crest; D014642:Vanilmandelic Acid / Q000592:standards / Q000652:urine*
D014642:Vanilmandelic Acid