Heavy and light chain sequences of four monoclonal antibodies that bind galactosylgloboside (GalGb4).
We recently described IgM monoclonal antibodies directed against the glycospingolipid galactosylgloboside (GalGb4; Marcus, M. D. et al., Arch. Biochem. Biophys, 1988.262: 620). We now present the nucleotide and deduced amino acid sequences of the heavy and light chains of these antibodies. The antibodies were generated in a single fusion, their heavy as well as their light chains are almost identical, and they appear to be clonally related. The light chains were encoded by J kappa 5 and a V kappa gene belonging to the Ox1 family, but they are only 93% homologous to the most closely related germ-line gene, and they are probably encoded by a germ-line gene that has not yet been identified. The heavy chains were all encoded by VH441 and JH2, and have identical N segments. The VH441 germ-line gene encodes a potential glycosylation site at Asn58 in the complementarity-determining region 2. This site, which has been retained in all VH441-encoded monoclonal antibodies sequenced previously, was mutated out by a single base change in all four anti-GalGb4 antibodies.
Snyder
J G
JG
Department of Medicine, Baylor College of Medicine, Houston, TX 77030.
Weng
N
N
Yu-Lee
L Y
LY
Marcus
D M
DM
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics*; D000918:Antibody Specificity; D015295:Antigens, Tumor-Associated, Carbohydrate; D001483:Base Sequence; D001666:Binding Sites, Antibody; D005803:Genes, Immunoglobulin; D006001:Glycoconjugates / Q000276:immunology; D006028:Glycosphingolipids / Q000276:immunology*; D007075:Immunoglobulin M / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D055746:Stage-Specific Embryonic Antigens
D000911:Antibodies, Monoclonal; D015295:Antigens, Tumor-Associated, Carbohydrate; D006001:Glycoconjugates; D006028:Glycosphingolipids; D007075:Immunoglobulin M; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains; D055746:Stage-Specific Embryonic Antigens; C052924:stage-specific embryonic antigen-3