Endothelin stimulates release of atrial natriuretic factor in anesthetized rats.
The present study was designed to examine the effect of synthetic rat endothelin, a novel potent vasoconstrictor isolated from endothelial cells, on the release of immunoreactive atrial natriuretic factor (ir-ANF) in anesthetized rats. Systemic blood pressure and plasma ir-ANF concentration were measured at 1, 3, 5, 15, 30, and 60 minutes after rat endothelin administration (0.01, 0.10, 1.00 nmol/kg body weight [BW]). Administration of rat endothelin caused a long-lasting increase in mean blood pressure and produced a dose-dependent increase in plasma ir-ANF that peaked 5 minutes after the injection and remained higher than the basal value 60 minutes after the injection. These data suggest that administration of pharmacological doses of rat endothelin produces a profound release of ir-ANF into the circulation, which may cause vasodilation and diuresis to antagonize the effect of endothelin.
Kohno
M
M
First Department of Internal Medicine, Osaka City University Medical School, Japan.
Murakawa
K
K
Horio
T
T
Kurihara
N
N
Yokokawa
K
K
Yasunari
K
K
Fukui
T
T
Takeda
T
T
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000097:blood / Q000378:metabolism*; D001794:Blood Pressure / Q000187:drug effects; D016232:Endothelins; D004730:Endothelium, Vascular / Q000378:metabolism; D008297:Male; D009994:Osmolar Concentration; D010455:Peptides / Q000494:pharmacology*; D011863:Radioimmunoassay; D051381:Rats
D016232:Endothelins; D010455:Peptides; D009320:Atrial Natriuretic Factor