[Immunochemical differences in the surface polysaccharides obtained from Entamoeba histolytica strain HM1:IMSS and its virulent (C-A) and non-virulent (L-6) clones].
There probably exist many different surface molecular polysaccharides with different compositions of their constituent sugars, in the different zymodemes. This fact might have a repercussion upon the resistance to the lysis of the complement. Seeking to prove this hypothesis, the ideal would be to obtain polysaccharide molecules of type LPFG of pathogenic and non-pathogenic zymodemes, demonstrating that there is a difference in the chemical composition of their polysaccharides. If this were true, a serologic classification, of the different zymodemes, would be possible in a fashion similar to gram-negative bacteria. Our results suggest the existence of structural differences between the polysaccharides of a virulent clone (C-A) and a non-virulent one (L-6).
Isibasi
A
A
Lab. de Inmunoquímica Unidad de Inv. biomédica, C.M.N., I.M.S.S., México, D.F.
Blanco
F
F
Arreguín
C
C
Martínez
G
G
Pelayo
R
R
Orozco
E
E
Kumate
J
J
D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology* / Q000302:isolation & purification; D000954:Antigens, Surface / Q000276:immunology* / Q000302:isolation & purification; D002999:Clone Cells / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004748:Entamoeba histolytica / Q000737:chemistry / Q000276:immunology* / Q000472:pathogenicity; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology / Q000302:isolation & purification; D008101:Liver Abscess, Amebic / Q000276:immunology; D010457:Peptidoglycan / Q000276:immunology* / Q000302:isolation & purification; D010743:Phospholipids / Q000276:immunology* / Q000302:isolation & purification; D011134:Polysaccharides / Q000276:immunology* / Q000302:isolation & purification; D014774:Virulence
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D008070:Lipopolysaccharides; D010457:Peptidoglycan; D010743:Phospholipids; D011134:Polysaccharides; C014643:lipopeptidophosphoglycan
Diferencias inmunoquímicas de polisacáridos de superficie obtenidos de Entamoeba histolytica cepa HM1:IMSS y sus clonas C-A virulenta y L-6 no virulenta.