title: A second molecular form of D2 dopamine receptor in rat and bovine caudate nucleus.
abstract: Overexpression of the D2 dopamine receptor has been proposed to be part of the pathology of schizophrenia. The isolation of a D2 dopamine receptor clone has assisted the molecular characterization of D2 receptor. We have now isolated an identical rat clone along with two other clones--a second related rat clone (RD-2in) and a homologous bovine clone (BD-2in), both of which contain an insert encoding an additional 29 amino acids relative to the original rat clone (RD-2o). All three clones encode D2 receptor binding sites when expressed in COS-7 cells. The amino-acid insert encoded by D-2in lies in the domain of the receptor believed to interact with the GTP-binding proteins (G proteins) of various signal transduction pathways. By using oligonucleotide probes specific for either D-2o or D-2in RNA transcripts, we have found that the level of expression of the D-2in-encoded form of the receptor is seven times that of the D-2o form in the caudate nucleus, the richest brain source of D2 receptors.
authors: Upjohn Co., Kalamazoo, Michigan 49001.
authors: C L
authors: CL
authors: Chio
authors: G F
authors: GF
authors: Hess
authors: R S
authors: RS
authors: Graham
authors: R M
authors: RM
authors: Huff
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D002421:Caudate Nucleus / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D051381:Rats; D011954:Receptors, Dopamine / Q000235:genetics* / Q000378:metabolism; D017448:Receptors, Dopamine D2; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013134:Spiperone / Q000378:metabolism; D014162:Transfection
chemical_list: D015345:Oligonucleotide Probes; D011954:Receptors, Dopamine; D017448:Receptors, Dopamine D2; D013134:Spiperone