title: Pathologic and enzyme histochemical studies on bone formation induced by bone morphogenetic protein in mouse muscle tissue.
abstract: Bone morphogenetic protein (BMP) irreversibly induced the differentiation of mesenchymal-type cells into osteoprogenitor cells for endochondral ossification. During the process of BMP-induced differentiation in mice, 4 cell type (chondroblasts, osteoblasts, chondroclasts, and osteoclasts) were examined for phosphatase and succinate dehydrogenase using a wide range of buffers (4.0 less than or equal to pH less than or equal to 9.2). During the chondroid tissue-forming stage (1 week), chondroblast-like or osteoblast-like cells expressed phosphatase activity at 6.8 less than or equal to pH less than or equal to 9.2; chondroclast-like or osteoclast-like cells expressed phosphatase activity at 4.0 less than or equal to pH less than or equal to 5.8. However, mature chondrocytes found in hyaline cartilage expressed phosphatase activity between 6.6 less than or equal to pH less than or equal to 7.6 (2 weeks). During the process of endochondral ossification, alkaline phosphatase activity decreased in osteoblast-like cells with traces of acid phosphatase activity still detectable. Chondroclastic and osteoclastic giant cells were characterized by intense succinate dehydrogenase activity.
authors: Department of Oral and Maxillofacial Surgery, Asahi University School of Dentistry, Gifu, Japan.
authors: A
authors: A
authors: Kamegai
authors: T
authors: T
authors: Tanabe
authors: K
authors: K
authors: Nagahara
authors: S
authors: S
authors: Kumasa
authors: M
authors: M
authors: Mori
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism; D000818:Animals; D001846:Bone Development / Q000187:drug effects*; D019485:Bone Morphogenetic Proteins; D002417:Cattle; D002455:Cell Division / Q000502:physiology; D004798:Enzymes / Q000032:analysis*; D006651:Histocytochemistry; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009132:Muscles / Q000187:drug effects / Q000201:enzymology*; D011506:Proteins / Q000494:pharmacology*; D013385:Succinate Dehydrogenase / Q000032:analysis / Q000378:metabolism
chemical_list: D019485:Bone Morphogenetic Proteins; D004798:Enzymes; D011506:Proteins; D013385:Succinate Dehydrogenase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase