Immunocytochemistry on fine needle aspirates in paraffin miniblocks.
A simplified method of processing of fine needle aspirates for paraffin miniblocks suitable for both morphologic and immunocytochemical evaluation is described. Aspirates were fixed in ethanol at 4 degrees C, dehydrated in acetone and xylene and embedded in paraffin (58 degrees C). All steps were carried out in a single Eppendorf centrifuge tube; the total process took less than four hours. Deparaffinized sections were stained using the alkaline phosphatase-antialkaline phosphatase technique with monoclonal and conventional antibodies helpful in the differential cytologic diagnosis of alcohol-fixed aspiration biopsy specimens. Antibodies to keratin, vimentin, desmin, neurofilaments, glial fibrillary acidic protein, leukocyte-common antigen, synaptophysin and immunoglobulin kappa and lambda light chains reacted positively on the miniblock material. Since the paraffin miniblocks combine the histologic pattern of the tumor with the differentiation-specific information provided by immunocytochemistry, their use can improve the accuracy of tumor typing in aspirates.
Domagala
W M
WM
Department of Tumor Pathology, Medical Academy, Szczecin, Poland.
Markiewski
M
M
Tuziak
T
T
Kram
A
A
Weber
K
K
Osborn
M
M
D015703:Antigens, CD / Q000276:immunology; D000943:Antigens, Differentiation / Q000276:immunology; D001707:Biopsy, Needle / Q000379:methods*; D006649:Histocompatibility Antigens / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007147:Immunoglobulin Light Chains / Q000276:immunology; D007381:Intermediate Filament Proteins / Q000276:immunology; D017493:Leukocyte Common Antigens; D008565:Membrane Proteins / Q000276:immunology; D009369:Neoplasms / Q000175:diagnosis* / Q000473:pathology; D010232:Paraffin; D016708:Synaptophysin
D015703:Antigens, CD; D000943:Antigens, Differentiation; D006649:Histocompatibility Antigens; D007147:Immunoglobulin Light Chains; D007381:Intermediate Filament Proteins; D008565:Membrane Proteins; D016708:Synaptophysin; D010232:Paraffin; D017493:Leukocyte Common Antigens