title: Lymphocyte subpopulations in peripheral blood of lambs experimentally infected with bovine respiratory syncytial virus.
abstract: The lymphocyte subpopulations of peripheral blood of normal lambs and lambs experimentally infected with bovine respiratory syncytial virus (RSV) were analysed by flow cytometry, using a panel of monoclonal antibodies against specific lymphocyte epitopes. Experimental infection with bovine RSV was characterized by a significant rise in SBU-T8+ (CD8+ or cytotoxic) T cells and a significant reduction in SBU-T4+ (CD4+ or helper) T cells and B (LCA p220+) lymphocytes (P less than 0.05). The helper/suppressor (CD4/CD8) ratio was reduced from 3.91 on the day of experimental infection to 1.13 on 10 days after experimental infection (P less than 0.001). The total number of SBU-T4+ (CD4+) and B cells returned to pre-inoculation values 14 days after experimental infection but the helper/suppressor ratio remained depressed up to 21 days post-inoculation.
authors: Department of Veterinary Pathology, University of Liverpool, Neston, Wirral, Great Britain.
authors: R
authors: R
authors: Sharma
authors: Z
authors: Z
authors: Woldehiwet
authors: D G
authors: DG
authors: Spiller
authors: H M
authors: HM
authors: Warenius
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes; D007958:Leukocyte Count / Q000662:veterinary; D008214:Lymphocytes; D012136:Respiratory Syncytial Viruses; D010253:Respirovirus Infections / Q000276:immunology / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic; D006377:T-Lymphocytes, Helper-Inducer
chemical_list: D000911:Antibodies, Monoclonal