[Use of enzyme preparation from Streptomyces recifensis subsp. lyticus for isolation of membrane substances from staphylococcal cells].
A procedure for isolating staphylococcal membranes including preprocessing of the cells with 0.1 M solution of cysteine hydrochloride and subsequent differential centrifugation was developed. The procedure is based on enzymatic lysis with an enzyme preparation from Streptomyces recifensis subsp. lyticus 2435. The membrane preparations had oxidase and dehydrogenase activity and were characterized by a high specific activity of the membrane-bound ATPase. Determination of the cytochrome differential spectra revealed the presence of cytochromes a, b and o in the membrane preparations.
Oriabinskaia
L B
LB
Babenko
Iu S
IuS
Vinnikov
A I
AI
D000251:Adenosine Triphosphatases / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000302:isolation & purification*; D001433:Bacteriolysis / Q000502:physiology*; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism; D003470:Culture Media; D003580:Cytochromes / Q000302:isolation & purification*; D066298:In Vitro Techniques; D010088:Oxidoreductases / Q000302:isolation & purification*; D013211:Staphylococcus aureus / Q000201:enzymology / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology*
D001426:Bacterial Proteins; D003470:Culture Media; D003580:Cytochromes; D010088:Oxidoreductases; D000251:Adenosine Triphosphatases
Ispol'zovanie fermentnogo preparata shtamma Streptomyces recifensis subsp. lyticus dlia vydeleniia membrannykh preparatov iz klerok stafilokokka.