Structure of germ line immunoglobulin alpha heavy-chain RNA and its location on polysomes.
We describe the structure of the major germ line RNA transcribed from unrearranged immunoglobulin alpha heavy-chain genes in immunoglobulin M-expressing cells of the I.29 mu B-cell lymphoma, a cell line capable of switching to immunoglobulin A expression upon lipopolysaccharide treatment. This germ line alpha RNA has a small open reading frame that does not include the C alpha domain, and this RNA appears to be present on polysomes in I.29 mu cells.
Radcliffe
G
G
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655.
Lin
Y C
YC
Julius
M
M
Marcu
K B
KB
Stavnezer
J
J
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D015332:Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor; D005803:Genes, Immunoglobulin; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007137:Immunoglobulin alpha-Chains / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011132:Polyribosomes / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping
D007143:Immunoglobulin Heavy Chains; D007137:Immunoglobulin alpha-Chains; D012333:RNA, Messenger