Down modulation of HIV-1 gene expression using a procaryotic RNA-binding protein.
The coat protein of the single stranded RNA bacteriophages acts as a translational repressor by binding with high affinity to a target RNA that encompasses the ribosomal binding site of the replicase gene. We have expressed this procaryotic RNA-binding protein in mammalian cells. Using the coat protein binding site attached to the HIV-1 5' leader RNA, we tested for the biological effect of co-expressed bacteriophage protein. We found that HIV-1 LTR-directed expression within this context was inhibited in trans by the coat protein. This example suggests the feasibility of using procaryotic RNA-binding proteins as genetic modulators in eucaryotic cells.
Berkhout
B
B
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
Jeang
K T
KT
D000595:Amino Acid Sequence; D000818:Animals; D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites; D002213:Capsid / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D015497:HIV-1 / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000378:metabolism*; D016601:RNA-Binding Proteins; D012091:Repetitive Sequences, Nucleic Acid; D014162:Transfection
D002352:Carrier Proteins; D012367:RNA, Viral; D016601:RNA-Binding Proteins