title: Occurrence of cytochrome b558 in B-cell lineage of human lymphocytes.
abstract: Cytochrome b558, involved in superoxide generation in phagocytes, was found to be expressed on the cell surface of most normal peripheral B lymphocytes. The cytochrome was not found on the surface of peripheral T lymphocytes, natural killer cells, or peripheral lymphocytes derived from patients with X-linked chronic granulomatous disease. On stimulation, at least half of peripheral B lymphocytes could generate superoxide anion as detected by superoxide dismutase-sensitive nitroblue tetrazorium reduction. Cytochrome b558 was not present on the surface of pre-pre B cells or pre-B cells, but did appear at the early B-cell stage. It disappeared from the B-cell surface during terminal differentiation to plasma cells. The transient expression of the cytochrome in B-cell lineage may indicate that superoxide generation is important for the function of these cells at certain stages.
authors: Institute of Medical Science, University of Tokyo, Minatoku, Japan.
authors: S
authors: S
authors: Kobayashi
authors: S
authors: S
authors: Imajoh-Ohmi
authors: M
authors: M
authors: Nakamura
authors: S
authors: S
authors: Kanegasaki
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000166:cytology / Q000502:physiology*; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D003573:Cytochrome b Group / Q000378:metabolism*; D005434:Flow Cytometry; D006410:Hematopoiesis; D006801:Humans; D019255:NADPH Oxidases; D013481:Superoxides / Q000378:metabolism; D013601:T-Lymphocytes / Q000502:physiology
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D003573:Cytochrome b Group; D013481:Superoxides; C036888:cytochrome b558; D019255:NADPH Oxidases