Liquid scintillation counting of 3H-thymidine incorporated into rat lens DNA.
DNA synthesis in the lens has previously been localized by autoradiography following incorporation of 3H-thymidine. For the quantification of DNA synthesis in the lens, pooling of lenses and extraction of the DNA for liquid scintillation counting, has formerly been adapted. In the present investigation a method has been developed for the extraction of the unincorporated tracer from whole lenses after short time incubation in a medium containing 3H-thymidine. The 3H-thymidine incorporated into individual lenses was then detected by liquid scintillation counting after dissolution of the lenses. The sources of the variation in the method are evaluated.
Söderberg
P G
PG
Department of Medical Biophysics, Karolinska Institute, Stockholm, Sweden.
Lindström
B
B
D000818:Animals; D004247:DNA / Q000096:biosynthesis*; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012588:Scintillation Counting; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D014316:Tritium; D004247:DNA; D013936:Thymidine