Modifications to the lysine Sepharose method of plasminogen purification which ensure plasmin-free Glu-plasminogen.
Modifications to the commonly employed lysine Sepharose affinity chromatography method for the purification of plasminogen from human plasma, give a preparation of native, Glu-plasminogen which is free of plasmin contamination.
Grant
A J
AJ
Department of Pharmacy, University of Sydney, New South Wales, Australia.
D000595:Amino Acid Sequence; D002846:Chromatography, Affinity / Q000379:methods; D003956:Dialysis; D005341:Fibrinolysin; D006801:Humans; D008969:Molecular Sequence Data; D010958:Plasminogen / Q000302:isolation & purification*; D012685:Sepharose / Q000031:analogs & derivatives
C030675:lysine-sepharose; D010958:Plasminogen; D012685:Sepharose; D005341:Fibrinolysin