A study on an enhancer-like element in Escherichia coli.
An enhancer-like element of 1.0kb (JM103-M) from E. coli JM103 chromosome was isolated using an enhancer-probing vector. The JM103-M fragment was shown to stimulate the nearby CAT or beta-galactosidase gene expression in E. coli in both directions at a stimulation rate of 3-6. The same fragment was also demonstrated to have promoter function when a promoter-probing vector was tested. Results obtained from sequencing data showed that a sequence TGACTAA homologous to the AP-1 binding DNA motif of SV40 and two GC boxes were present.
Pan
W
W
National Laboratory of Molecular Virology and Genetic Engineering, Beijing.
Wu
S H
SH
Jin
Q
Q
Yu
W H
WH
Liu
Z W
ZW
Jin
D Y
DY
Du
P
P
Hou
Y D
YD
D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004742:Enhancer Elements, Genetic; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D001616:beta-Galactosidase / Q000235:genetics
D004269:DNA, Bacterial; D015500:Chloramphenicol O-Acetyltransferase; D001616:beta-Galactosidase