Biochemical and physiological responses of Tetrahymena pyriformis during adaptation to high glycerol concentrations.
Tetrahymena pyriformis grown in high glycerol concentrations (0.6 and 0.8 M) have lower a growth rate than controls. There is no detectable difference in the electrophoretic pattern of unlabeled lysates of cells adapted to high glycerol concentrations in comparison with controls. On the contrary, the electrophoretic pattern of lysates of labeled Tetrahymena pyriformis revealed a set of proteins which are synthesized more vigorously in the glycerol adapted cells. Evidence is presented that some of these peptides are distributed in the mitochondrial-lysosomal fraction of glycerol adapted cells.
Gounaris
E G
EG
Aristotelian University of Thessaloniki, School of Chemistry, Laboratory of Biochemistry, Greece.
Bardopoulou
H
H
Kotinis
K K
KK
D000222:Adaptation, Physiological; D000818:Animals; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D005990:Glycerol / Q000378:metabolism*; D008247:Lysosomes / Q000737:chemistry; D008928:Mitochondria / Q000737:chemistry; D009994:Osmolar Concentration; D015800:Protozoan Proteins / Q000096:biosynthesis; D013769:Tetrahymena pyriformis / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*
D003470:Culture Media; D015800:Protozoan Proteins; D005990:Glycerol