Changes in total fiber numbers of disused soleus muscle on rats.
In this study, by use of technique that was modified from Morey method, we discussed the histological influence on the soleus muscle of the rats caused by disuse. This study is characterized by the calculating of total numbers of muscle fibers. ST (slow-twitch) and FT (fast-twitch) fibers in total muscular cross-sectional area were classified by their difference in intensity of staining of actomyosin adenosinetriphosphatase (myosin ATPase). During the experiment, average fiber diameter of ST and FT fibers declined when compared to control group (p less than 0.01). A 54% decrease in the total number of ST fibers was observed in the experimental group (p less than 0.01). Conversely, the total number of FT fibers increased to 362% of the control value (p less than 0.01). These results of the changes evoked in ST and FT fibers indicate 34% decrease in total muscular cross-sectional area, and showed that muscular function shifted toward a faster muscle in disused soleus muscle.
Nakagawa
Y
Y
Department of Health and Exercise Sciences, Otaru University of Commerce, Japan.
Morii
H
H
Totsuka
M
M
Sato
T
T
Hirota
K
K
D000818:Animals; D006651:Histocytochemistry; D007866:Leg; D008297:Male; D009132:Muscles / Q000201:enzymology / Q000473:pathology*; D009133:Muscular Atrophy / Q000473:pathology; D009218:Myosins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012149:Restraint, Physical
D009218:Myosins