Purification, cloning, expression and biological characterization of an interleukin-1 receptor antagonist protein.
A human myelomonocytic cell line, U937, produced an interleukin-1 (IL-1) receptor antagonist protein (IRAP) which was purified and partially sequenced. A complementary DNA coding for IRAP was cloned and sequenced. The mature translation product of the cDNA has been expressed in Escherichia coli and was an active competitive inhibitor of the binding of IL-1 to the T-cell/fibroblast form of the IL-1 receptor. Recombinant IRAP specifically inhibited IL-1 bioactivity on T cells and endothelial cells in vitro and was a potent inhibitor of IL-1 induced corticosterone production in vivo.
Carter
D B
DB
Department of Molecular Biology Research, Upjohn Company, Kalamazoo, Michigan 49007.
Deibel
M R
MR
Dunn
C J
CJ
Tomich
C S
CS
Laborde
A L
AL
Slightom
J L
JL
Berger
A E
AE
Bienkowski
M J
MJ
Sun
F F
FF
McEwan
R N
RN
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D002448:Cell Adhesion; D002460:Cell Line; D003001:Cloning, Molecular; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D003345:Corticosterone / Q000097:blood; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006098:Granulocytes / Q000378:metabolism; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000378:metabolism / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D009000:Monocytes / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology / Q000502:physiology; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology; D011971:Receptors, Immunologic / Q000037:antagonists & inhibitors* / Q000378:metabolism; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins; D012795:Sialoglycoproteins; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D003115:Colony-Stimulating Factors; D006133:Growth Substances; C507763:IL1RN protein, human; C507764:Il1rn protein, mouse; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D011506:Proteins; D011971:Receptors, Immunologic; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins; D012795:Sialoglycoproteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D004247:DNA; D013755:Tetradecanoylphorbol Acetate; D003345:Corticosterone