[A rat model for the study of Pseudomonas aeruginosa enterogenous infection after severe scald].
Eighty eight healthy Wistar rats were used in this study. Pseudomonas aeruginosa (serotype IX, labelled with fluorescein isothiocyanate) were fed in suspension (10(9)/ml, 1ml/100g body weight) following a 25-28% full-thickness burn created on the back of animals. The animals were sacrificed at eight different time phases from 1-72 hours after burns. The labelled bacteria were traced in internal organs, the quantitative bacterial cultures of visceral tissues and blood cultures were performed coincidently. The results showed that the labelled bacteria introduced into GI tract could be demonstrated from the internal organs and blood circulation.
Zhang
Y P
YP
Burn Research Institute, Third Military Medical College, Chongqing.
D000818:Animals; D002056:Burns / Q000150:complications*; D004064:Digestive System / Q000382:microbiology; D004195:Disease Models, Animal; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D008297:Male; D011552:Pseudomonas Infections / Q000209:etiology* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains; D013861:Thiocyanates
D005452:Fluoresceins; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate