Recent progress in mite allergen immunochemistry.
The successful purification of several mite allergens within the last few years has considerably enhanced our understanding of mite allergen immunochemistry. The role of these glycoproteins in stimulating human IgE ab and their role as immunogens in mice and rabbits has been studied extensively in a number of laboratories worldwide. In particular, purified allergens have facilitated the production of murine IgG Mabs that have been used to purify mite allergens by affinity chromatography; to investigate the diversity of antigenic sites on purified allergens; and to develop Mab based immunoassays for measuring allergen concentrations in dust samples and extracts. Full amino acid sequencing of several mite allergens is now in progress together with efforts to identify antigenically important peptide fragments. Such investigations are aimed to further increase our knowledge of humoral and cellular immune responses at the molecular level. For years, pollen counts have been used to judge airborne pollen allergen levels and to predict, in turn, the severity of symptom days for patients with hay fever. In contrast, simple methods for measuring dust allergens (e.g., mite allergen) have not been available. The development of Mab immunoassays, which can be converted from radiolabeled to enzyme labeled or fluorescence labeled assays, should provide rapid and quantitative measurements of specific mite allergen levels in house dust. Not only can such measurements provide useful clinical information in judging the exposure of patients to mite allergen, but the effectiveness of allergen avoidance regimes can be monitored objectively. By measuring the concentration of specific allergens in extracts, these assays could significantly improve efforts to standardize extracts used for diagnosis and treatment.
Heymann
P W
PW
Division of Allergy and Clinical Immunology, University of Virginia Medical Center, Charlottesville 22908.
Chapman
M D
MD
D000485:Allergens / Q000276:immunology*; D000818:Animals; D006801:Humans; D007120:Immunochemistry; D008925:Mites / Q000276:immunology*; D012130:Respiratory Hypersensitivity / Q000209:etiology / Q000276:immunology
D000485:Allergens