Isolation of recessive (mediator-) revertants from NIH 3T3 cells transformed with a c-H-ras oncogene.
We have generated two serum- and anchorage-dependent revertants from NIH 3T3 cells transformed with multiple copies of the human c-H-ras oncogene. In both revertants, the c-H-ras oncogene was fully expressed. Fusion of either revertant with untransformed cells or of the two revertants with one another resulted in transformed progeny. These results indicated that the two revertants were recessive and in different complementation groups. We believe that in our two revertants some of the genes mediating the transforming activity of the c-H-ras oncogene are defective; we are attempting to identify these mediator genes.
Yamada
H
H
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511.
Omata-Yamada
T
T
Wakabayashi-Ito
N
N
Carter
S G
SG
Lengyel
P
P
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D005808:Genes, Recessive; D011905:Genes, ras; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology; D051379:Mice; D015689:Oncogene Protein p21(ras) / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection; D001749:Urinary Bladder Neoplasms
D012333:RNA, Messenger; D015689:Oncogene Protein p21(ras)