Maintenance of plasmids in HU and IHF mutants of Escherichia coli.
Complementation and sequencing analyses revealed that the hopD mutants, which could not support stable maintenance of mini-F plasmids (Niki et al. 1988), had mutations in the hupB gene, and that the hopD410 mutation was an ochre mutation at the 5th Gln position of HU-1. Maintenance and stability of various plasmids, mini-P1 plasmids, mini-F plasmids, and oriC plasmids, were studied in the hupA and hupB mutants (HU mutants), and himA and hip mutants (IHF mutants). Mini-P1 plasmids and mini-F plasmids could not be introduced into the delta hupA-delta hupB double deletion mutant. Replication of mini-F plasmids was partially inhibited in the hupB mutants, including the delta hupB and hopD(hupB) mutants, whereas replication of oriC plasmids was not significantly affected even in the delta hupA-delta hupB double deletion mutant. The mini-P1 plasmid was slightly unstable in the himA-hip mutant, whereas the mini-F plasmid was stable.
Ogura
T
T
Department of Molecular Genetics, Kumamoto University Medical School, Japan.
Niki
H
H
Kano
Y
Y
Imamoto
F
F
Hiraga
S
S
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D003086:Bacteriocin Plasmids; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005144:F Factor; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D034982:Integration Host Factors; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D015183:Restriction Mapping; D013997:Time Factors; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D034982:Integration Host Factors; C040330:histone-like protein HU, bacteria