title: An adenovirus recombinant that expresses the human cytomegalovirus major envelope glycoprotein and induces neutralizing antibodies.
abstract: The gene of the human cytomegalovirus (HCMV) major envelope glycoprotein, gB, was cloned from the Towne strain and inserted into adenovirus type 5 downstream of the E3 promoter. The recombinant virus, Ad-gB, expressed antigenically related proteins of 58, 30, 25, and 23 kDa in A549 and MRC-5 cells; the 58-kDa protein had the same mobility as the native gB from HCMV-infected MRC-5 cells and virions. All four proteins were detected by a monospecific polyclonal antiserum and by a monoclonal antibody in immunoblot and immunofluorescence assays. Hamsters infected intranasally with live Ad-gB developed protein-specific and HCMV-neutralizing antibody. This study confirms the importance of gB in the generation of the neutralizing immune response to HCMV and demonstrates the potential of live adenoviruses as vaccine vectors.
authors.affiliation: Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
authors.forename: G S
authors.initials: GS
authors.lastname: Marshall
authors.forename: R P
authors.initials: RP
authors.lastname: Ricciardi
authors.forename: R F
authors.initials: RF
authors.lastname: Rando
authors.forename: J
authors.initials: J
authors.lastname: Puck
authors.forename: R W
authors.initials: RW
authors.lastname: Ge
authors.forename: S A
authors.initials: SA
authors.lastname: Plotkin
authors.forename: E
authors.initials: E
authors.lastname: Gönczöl
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics* / Q000276:immunology; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D015139:Blotting, Southern; D003001:Cloning, Molecular; D006224:Cricetinae; D003587:Cytomegalovirus / Q000235:genetics* / Q000276:immunology; D005455:Fluorescent Antibody Technique; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D006801:Humans; D015151:Immunoblotting; D015345:Oligonucleotide Probes; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D015345:Oligonucleotide Probes; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus