Lymphocyte HEV adhesion variants differ in the expression of multiple gene sequences.
Lymphocyte adhesion to high endothelial venule cells in lymphoid organs of mice is mediated by several cell-surface glycoproteins, one of which, gp90MEL-14, is detected by the MEL-14 monoclonal antibody (mAb). The MEL-14 mAb was used to select two variants of the EL4 cell line, EL4MEL-14-hi and EL4-MEL-14-lo, that have disparate cell surface expression of this adhesion receptor. A cDNA library constructed from EL4MEL-14-hi mRNA was enriched for sequences present at higher levels in EL4MEL-14-hi cells than EL4MEL-14-lo cells. Quantitative analysis of candidate differential clones by RNA probe protection methods identified five clones whose steady-state mRNA levels were increased in the EL4MEL-14-hi cells. One of these clones, DIFF6, is derived from an RNA whose expression level is higher in several cell lines producing high amounts of MEL-14-reactive gp90, and absent or present at lower levels in several cell lines expressing low levels of this glycoprotein. However, DIFF6 does not encode gp90MEL-14. The nucleotide sequence of this clone predicts a relatively hydrophilic protein characteristic of a cytoplasmic or nuclear protein. Present experiments indicate that expression of gp90MEL-14, a cell-surface-adhesion receptor molecule, may be coregulated with additional cytoplasmic or nuclear factors.
Nottenburg
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Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
Gallatin
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St John
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D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins; D004251:DNA Transposable Elements; D005455:Fluorescent Antibody Technique; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D005786:Gene Expression Regulation; D051379:Mice; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D016028:Receptors, Leukocyte-Adhesion / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D058112:Septins; D014407:Tumor Cells, Cultured; D014699:Venules / Q000378:metabolism
D000911:Antibodies, Monoclonal; D003598:Cytoskeletal Proteins; D004251:DNA Transposable Elements; D011506:Proteins; D016028:Receptors, Leukocyte-Adhesion; C491457:Sept1 protein, mouse; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D058112:Septins