Ectopic expression of c-jun leads to differentiation of P19 embryonal carcinoma cells.
The product of the c-jun proto-oncogene, a major component of the transcription factor AP1, has been implicated in both positive and negative transcriptional control as well as in transformation. The role of c-jun in early development and differentiation processes, however, is still largely unknown. In this paper we show that ectopic expression of exogenous c-jun sequences leads to differentiation and loss of the transformed phenotype of P19 embryonal carcinoma (EC) cells. The mixed populations of endoderm- and mesoderm-like cells that were obtained after the introduction of c-jun morphologically and biochemically resembled P19 cells differentiated in monolayer by retinoic acid (RA). Furthermore, we provide evidence that direct effects of the c-jun gene product on transcription of the retinoic acid receptor beta (RAR beta) gene may be responsible for the differentiation-promoting potential of c-jun.
de Groot
R P
RP
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.
Kruyt
F A
FA
van der Saag
P T
PT
Kruijer
W
W
D002352:Carrier Proteins / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D004247:DNA / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D054278:Embryonal Carcinoma Stem Cells; D006801:Humans; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000187:drug effects; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics; D016755:Proto-Oncogene Proteins c-jun; D011519:Proto-Oncogenes; D018168:Receptors, Retinoic Acid; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D014162:Transfection; D014212:Tretinoin / Q000494:pharmacology
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D016755:Proto-Oncogene Proteins c-jun; D018168:Receptors, Retinoic Acid; D014157:Transcription Factors; D014212:Tretinoin; D004247:DNA