Developmental changes in the regulation of glycine-activated Cl- channels of cultured rat medullary neurons.
Glycine-activated currents in 1- to 11-day-old rat medullary neurons were studied using patch clamp techniques. Glycine produced neither repeatable whole-cell current responses nor single-channel activity in the cell-attached mode until cells were in culture for a week or more. However, Cl- channels were present at the early stages because glycine-activated channels were seen in excised, inside-out patches. Furthermore, for cells less than a week in culture, 10 patches which did not exhibit glycine-activated Cl- channels in the cell-attached mode did upon excision. Consequently, the activation properties of these Cl- channels undergo a developmental change in that some cellular factor(s) presumably prevents the Cl- channels from opening in the intact cell during the initial stages in culture.
Lewis
C A
CA
Department of Physiology, State University of New York, Buffalo 14214.
Ahmed
Z
Z
Faber
D S
DS
D000818:Animals; D002478:Cells, Cultured; D018118:Chloride Channels; D002712:Chlorides / Q000502:physiology*; D005998:Glycine / Q000494:pharmacology*; D008526:Medulla Oblongata / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects; D008565:Membrane Proteins / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains
D018118:Chloride Channels; D002712:Chlorides; D008565:Membrane Proteins; D005998:Glycine