T lymphocytes possess receptors for brain myelin small protein.
In immunomediated demyelinating diseases, T cells are found in chronic lesions. To discover whether immunocompetent cells may interact with some myelin proteins, we purified myelin proteins in the lipid-bound native state and evaluated their binding to peripheral blood mononuclear cells (PMBC) isolated from healthy donors. To this end, myelin proteins were conjugated to biotin and added to PBMCs or purified CD4+ and CD8+ cells; then binding was detected using fluoresceinated avidin. In this article, we describe experiments carried out with a myelin protein recently discovered in the central nervous system. Our results show that this small, phosphatidylserine-binding protein can bind to human T cells.
Riccio
P
P
Dipartimento di Biochimica e Biologia Molecolare, Universitá di Bari, Italy.
Jirillo
E
E
Bobba
A
A
Munno
I
I
Colangelo
A M
AM
D000945:Antigens, Differentiation, T-Lymphocyte; D001665:Binding Sites; D015704:CD4 Antigens; D016827:CD8 Antigens; D003711:Demyelinating Diseases / Q000276:immunology / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D009185:Myelin Proteins / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*
D000945:Antigens, Differentiation, T-Lymphocyte; D015704:CD4 Antigens; D016827:CD8 Antigens; D009185:Myelin Proteins; D011956:Receptors, Cell Surface