Mannose-6-phosphate stimulates proliferation of neuronal precursor cells.
The mitogenic signal function of mannose-6-phosphate (Man-6-P)/insulin-like growth factor II (IGF-II) receptors was studied in neuronal precursor cells from developing rat brain (E15). About 30% of the cellular Man-6-P/IGF-II receptors were present on the cell surface. Man-6-P and IGF-II stimulated DNA synthesis twofold and their effects were additive. Antibody 3637 to the Man-6-P/IGF-II receptor blocked the response to Man-6-P but not that to IGF-II. Other phosphorylated hexoses were also active. Fructose-1-phosphate was equally potent with Man-6-P, whereas glucose-6-phosphate was 5 times less potent. We conclude that Man-6-P-containing proteins and IGF-II act as mitogens in developing brain by interaction with the Man-6-P/IGF-II receptor and the IGF-I receptor, respectively.
Nielsen
F C
FC
Department of Clinical Chemistry, Bispebjerg Hospital, Copenhagen NV, Denmark.
Gammeltoft
S
S
D000818:Animals; D004247:DNA / Q000096:biosynthesis; D006600:Hexosephosphates / Q000494:pharmacology*; D008360:Mannosephosphates / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects*; D051381:Rats; D017527:Receptor, IGF Type 2; D011956:Receptors, Cell Surface / Q000032:analysis / Q000276:immunology; D017451:Receptors, Somatomedin; D013234:Stem Cells / Q000187:drug effects*
D006600:Hexosephosphates; D008360:Mannosephosphates; D017527:Receptor, IGF Type 2; D011956:Receptors, Cell Surface; D017451:Receptors, Somatomedin; C027693:mannose-6-phosphate; D004247:DNA