On the purification of notexin. Isolation of a single amino acid variant from the venom of Notechis scutatus scutatus.
Venom of the Australian tiger snake, Notechis scutatus scutatus was fractionated by conventional ion-exchange chromatography. The fraction containing notexin, a well-known single-chain toxic phospholipase A2, was further purified by reverse-phase high-performance liquid chromatography. Two main components were isolated and the major one corresponded to notexin. The other component, designated as notechis Ns, was an isoform of notexin. Notechis Ns and notexin possessed similar in vitro esterase activity, in vitro neuromuscular activity and in vivo lethality. Amino acid composition and sequence of the Staphylococcus aureus V8-protease peptides demonstrated that primary structures of notechis Ns and notexin differed from each other by a single substitution amongst 119 amino acids: Lys----Arg at position 16.
Chwetzoff
S
S
Département de Biologie, CEN de Saclay, Gif sur Yvette, France.
Mollier
P
P
Bouet
F
F
Rowan
E G
EG
Harvey
A L
AL
Ménez
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001681:Biological Assay; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004546:Elapid Venoms / Q000302:isolation & purification* / Q000494:pharmacology / Q000633:toxicity; D004950:Esterases / Q000378:metabolism; D005260:Female; D006868:Hydrolysis; D007928:Lethal Dose 50; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009469:Neuromuscular Junction / Q000187:drug effects / Q000502:physiology; D010446:Peptide Fragments; D011894:Rana pipiens; D009435:Synaptic Transmission / Q000187:drug effects
D004546:Elapid Venoms; D010446:Peptide Fragments; C005082:notexin; D004950:Esterases