The ammonium sulfate activation of phosphorylase b.
The ammonium sulfate activation of phosphorylase b has been studied. Ammonium sulfate, when present in high concentrations, induces properties of phosphorylase a in phosphorylase b, such as an enhanced affinity for AMP, a reversal of the glucose-6-P inhibition and enzyme tetramerization. The data are consistent with the interpretation that sulfates bind to the Ser-14 site and the sulfate-protein interactions at this site are responsible for activation of phosphorylase b.
Leonidas
D D
DD
Biological Research Center, National Hellenic Research Foundation, Athens, Greece.
Oikonomakos
N G
NG
Papageorgiou
A C
AC
Xenakis
A
A
Cazianis
C T
CT
Bem
F
F
D000249:Adenosine Monophosphate / Q000494:pharmacology; D000645:Ammonium Sulfate / Q000494:pharmacology*; D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D004789:Enzyme Activation / Q000187:drug effects; D005958:Glucosephosphates / Q000378:metabolism; D007700:Kinetics; D046911:Macromolecular Substances; D009132:Muscles / Q000201:enzymology*; D010763:Phosphorylase b / Q000378:metabolism*; D006005:Phosphorylases / Q000378:metabolism*; D011817:Rabbits; D014461:Ultracentrifugation
D005958:Glucosephosphates; D046911:Macromolecular Substances; D000249:Adenosine Monophosphate; C031590:glucose-1-phosphate; D010763:Phosphorylase b; D006005:Phosphorylases; D000645:Ammonium Sulfate