Glucocorticoids regulate expression of the v-mycOK10 oncogene in a murine retroviral vector with chimeric MoMuLV-MMTV LTRs.
A murine retrovirus which expresses the v-mycOK10 oncogene under the control of the dexamethasone-regulatable mouse mammary tumor virus (MMTV) promoter has been constructed. In this vector, denoted pMImyc, the Moloney Murine leukemia virus (MoMuLV) sequences required for virus replication, integration and packaging were kept, while all the elements for transcription regulation were derived from the MMTV long terminal repeat (LTR). After transfection of NIH 3T3 fibroblasts with this construct, a cell line was isolated in which the level of v-myc RNAs were increased 60 fold by dexamethasone. Kinetic studies showed that this induction can be maintained for up to 12 hours of hormone treatment. After infection with MoMuLV as a helper virus, and in the presence of dexamethasone, the production of pMImyc RNA, estimated by slot blot analysis, was equivalent to about 10(3) viral particles/ml.
Jacquemin-Sablon
H
H
Molecular Biology and Virology Laboratory, Salk Institute, San Diego.
Bogenberger
J
J
D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D002678:Chimera / Q000235:genetics; D003907:Dexamethasone / Q000494:pharmacology*; D005347:Fibroblasts; D015972:Gene Expression Regulation, Neoplastic; D005822:Genetic Vectors; D007700:Kinetics; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics*; D051379:Mice; D008979:Moloney murine leukemia virus / Q000235:genetics*; D009857:Oncogenes; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic; D014162:Transfection
D003907:Dexamethasone