Influence of chain length of pyrene fatty acids on their uptake and metabolism by Epstein-Barr-virus-transformed lymphoid cell lines from a patient with multisystemic lipid storage myopathy and from control subjects.
The uptake and intracellular metabolism of 4-(1-pyrene)butanoic acid (P4), 10-(1-pyrene)decanoic acid (P10) and 12-(1-pyrene)dodecanoic acid (P12) were investigated in cultured lymphoid cell lines from normal individuals and from a patient with multisystemic lipid storage myopathy (MLSM). The cellular uptake was shown to be dependent on the fatty-acid chain length, but no significant difference in the uptake of pyrene fatty acids was observed between MLSM and control lymphoid cells. After incubation for 1 h the distribution of fluorescent fatty acids taken up by the lymphoid cell lines also differed with the chain length, most of the fluorescence being associated with phospholipid and triacylglycerols. In contrast with P10 and P12, P4 was not incorporated into neutral lipids. When the cells were incubated for 24 h with the pyrene fatty acids, the amount of fluorescent lipids synthesized by the cells was proportional to the fatty acid concentration in the culture medium. After a 24 h incubation in the presence of P10 or P12, at any concentration, the fluorescent triacylglycerol content of MLSM cells was 2-5-fold higher than that of control cells. Concentrations of pyrene fatty acids higher than 40 microM seemed to be more toxic for mutant cells than for control cells. This cytotoxicity was dependent on the fluorescent-fatty-acid chain length (P12 greater than P10 greater than P4). Pulse-chase experiments permitted one to demonstrate the defect in the degradation of endogenously biosynthesized triacylglycerols in MLSM cells (residual activity was around 10-25% of controls on the basis of half-lives and initial rates of P10- or P12-labelled-triacylglycerol catabolism); MLSM lymphoid cells exhibited a mild phenotypic expression of the lipid storage (less severe than that observed in fibroblasts). P4 was not utilized in the synthesis of triacylglycerols, and thus did not accumulate in MLSM cells: this suggests that natural short-chain fatty acids might induce a lesser lipid storage in this disease.
Radom
J
J
Laboratoire de Biochimie, Faculté de Médecine-Rangueil, Toulouse, France.
Salvayre
R
R
Levade
T
T
Douste-Blazy
L
L
D001402:B-Lymphocytes / Q000378:metabolism*; D002087:Butyrates / Q000378:metabolism; D020148:Butyric Acid; D002461:Cell Line, Transformed; D003652:Decanoic Acids / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D005456:Fluorescent Dyes; D004854:Herpesvirus 4, Human; D006801:Humans; D007700:Kinetics; D007850:Lauric Acids / Q000378:metabolism; D050356:Lipid Metabolism; D008052:Lipid Metabolism, Inborn Errors / Q000150:complications / Q000378:metabolism*; D015394:Molecular Structure; D009135:Muscular Diseases / Q000209:etiology / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D011721:Pyrenes / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014280:Triglycerides / Q000378:metabolism
D002087:Butyrates; D003652:Decanoic Acids; D005227:Fatty Acids; D005456:Fluorescent Dyes; D007850:Lauric Acids; D010743:Phospholipids; D011721:Pyrenes; D014280:Triglycerides; D020148:Butyric Acid; C030358:lauric acid; C031071:decanoic acid