title: Transfer and expression of the tetracycline resistance transposon Tn925 in Acetobacterium woodii.
abstract: The Enterococcus faecalis conjugative plasmid pCF10 was used to introduce Tn925 into Acetobacterium woodii by filter mating. Tetracycline resistance was transferred at frequencies of about 10(-6) per donor, but no plasmid DNA was found in the transconjugants. DNA hybridization analyses of HindIII-digested chromosomal DNA demonstrated the insertion of Tn925 at a variety of locations, whereas wild type DNA showed no hybridization at all. The transconjugants were used as donor in mating experiments with tetracycline-sensitive Bacillus subtilis. Transfer of tetracycline resistance was observed at frequencies of 10(-8) per recipient.
authors.affiliation: Institut für Mikrobiologie, Universität Göttingen, F.R.G.
authors.forename: M
authors.initials: M
authors.lastname: Strätz
authors.forename: G
authors.initials: G
authors.lastname: Gottschalk
authors.forename: P
authors.initials: P
authors.lastname: Dürre
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics; D015139:Blotting, Southern; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D013293:Enterococcus faecalis / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D006094:Gram-Positive Bacteria / Q000235:genetics*; D010957:Plasmids; D013753:Tetracycline Resistance / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial