Role of oxygen radicals in ethanol-induced damage to cultured gastric mucosal cells.
We have examined the role of oxygen radicals in ethanol-induced damage to cultured rat gastric mucosal cells. Cultured cells exposed to ethanol produced superoxide anion, as assessed by the reduction of cytochrome c, in a time-related fashion. The production of superoxide anion increased dose dependently as the concentration of ethanol increased. Cellular damage increased in a similar fashion to the production of superoxide anion. Both superoxide dismutase (SOD) and catalase diminished ethanol-induced injury dose dependently. SOD and catalase were able to maintain their enzymatic activities in the presence of 15% ethanol, respectively. Pretreatment with deferoxamine, an iron-chelating agent, decreased ethanol-induced injury dose dependently. Furthermore, dimethyl sulfoxide decreased ethanol-induced damage dose dependently. We conclude that cultured gastric mucosal cells exposed to ethanol generate oxygen radicals and that the production of oxygen radicals is closely linked with ethanol-induced damage to the cells. Hydroxyl radical, produced by the iron-catalyzed Haber-Weiss reaction, seems to be the main mediator of ethanol-induced damage to gastric mucosal cells in vitro.
Mutoh
H
H
Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Hiraishi
H
H
Ota
S
S
Ivey
K J
KJ
Terano
A
A
Sugimoto
T
T
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003574:Cytochrome c Group / Q000378:metabolism; D003676:Deferoxamine / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D005609:Free Radicals; D005753:Gastric Mucosa / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013482:Superoxide Dismutase / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism*
D003574:Cytochrome c Group; D005609:Free Radicals; D013481:Superoxides; D000431:Ethanol; D013482:Superoxide Dismutase; D003676:Deferoxamine; D004121:Dimethyl Sulfoxide