title: Amino acid sequence of a peptide with both molt-inhibiting and hyperglycemic activities in the lobster, Homarus americanus.
abstract: A hydrophobic peptide of 71 residues was isolated from lobster sinus gland extracts that prolonged intermolt periods and lowered ecdysteroid titers in juvenile lobsters. Removal of the N-terminal pyroglutamyl residue allowed sequencing of 30 of the first 36 residues. Additional data were obtained from HPLC-purified fragments from endoproteinase cleavages (Lys-C, Glu-C, Arg-C, Asp-N), and carboxypeptidase Y digestion. This is the first reported amino acid sequence of a crustacean molt-inhibiting hormone. This peptide also has significant hyperglycemic activity.
authors.affiliation: Bodega Marine Laboratory, University of California, Bodega Bay 94923.
authors.forename: E S
authors.initials: ES
authors.lastname: Chang
authors.forename: G D
authors.initials: GD
authors.lastname: Prestwich
authors.forename: M J
authors.initials: MJ
authors.lastname: Bruce
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003488:Cyanogen Bromide; D005947:Glucose / Q000378:metabolism*; D006458:Hemolymph / Q000187:drug effects / Q000378:metabolism; D007447:Invertebrate Hormones; D008969:Molecular Sequence Data; D008121:Nephropidae / Q000254:growth & development / Q000502:physiology*; D009479:Neuropeptides / Q000302:isolation & purification / Q000378:metabolism*; D010447:Peptide Hydrolases; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000378:metabolism*; D051236:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide; D011979:Receptors, Pituitary Hormone
chemical_list: C065680:Hoa-MIH; D007447:Invertebrate Hormones; D009479:Neuropeptides; D010455:Peptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D011956:Receptors, Cell Surface; D051236:Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide; D011979:Receptors, Pituitary Hormone; D010447:Peptide Hydrolases; D005947:Glucose; D003488:Cyanogen Bromide