Establishment and characterization of a primitive neuroectodermal tumor of bone continuous cell line (LAP-35).
A continuous tumor cell line (LAP-35) was established from a primitive neuroectodermal tumor of bone from the right tibia of a 12-year-old female. The neural character of the cell line was documented by the spontaneous growth of neurites and by the presence of several neural markers, including neuron-specific enolase (NSE), S-100 protein, neurofilaments, chromogranin A, synaptophysin and positivity to monoclonal antibodies UJ127.11, UJ13A, UJ181.4. Cell-sorter analysis showed a high expression of nerve growth factor receptor (NGFr) and major histocompatibility complex class I-related molecules. A unique cytogenetic profile was observed, including a reciprocal chromosomal translocation (rct) 11:22 (q24;q12), typically associated with Ewing's sarcoma and neuroepithelioma, and deletion of the short arm of chromosome 1 (lp-), otherwise a feature of neuroblastoma. N-myc proto-oncogene was neither amplified nor expressed, whereas the expression of c-myc was documented by northern blot analysis. These features distinguish this new cell line from previously reported neuroectodermal cell lines, identifying LAP-35 as a unique model of a group of neural bone tumors that share characteristics of neuroblastoma as well as neuroepithelioma.
Bagnara
G P
GP
G. Prodi Interdepartmental Center for Cancer Research, Bologna, Italy.
Serra
M
M
Giovannini
M
M
Badiali
M
M
Stella
M
M
Montaldi
A
A
Granchi
D
D
Paolucci
P
P
Rocchi
P
P
Pession
A
A
D000818:Animals; D001859:Bone Neoplasms / Q000473:pathology*; D002455:Cell Division / Q000502:physiology; D002648:Child; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D016259:Genes, myc / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D009380:Neoplasms, Nerve Tissue / Q000473:pathology*; D000090063:Proto-Oncogene Mas; D014407:Tumor Cells, Cultured / Q000166:cytology*