Two novel c-abl mRNAs are expressed in rat parotid salivary glands during in vivo beta-adrenergic receptor stimulation.
The c-abl proto-oncogene is transcribed in most cell lines and tissues into two mRNAs of 6.5 and 5.3 kb, which have different 5' ends and encode two 150 kDa proteins that are largely colinear, but have different N-termini. We show here that two unusually short and abundant c-abl-related mRNAs of 1.5 and 1.3 kb appear in rat parotid salivary glands, within 1 day of in vivo administration of the beta-adrenergic receptor agonist isoproterenol. These transcripts are not found in the submandibular salivary gland or in the heart and they are too short to encode the known c-abl proteins. RNA blot, S1 nuclease protection and primer extension analysis suggest that the isoproterenol inducible parotid gland mRNAs do not contain the kinase domain, but represent part of the C-terminal segment of the abl reading frame.
Mertz
P M
PM
Clinical Investigations and Patient Care Branch, National Institute of Dental Research, NIH, Bethesda, MD 20832.
Backman
T
T
Bernards
A
A
Kousvelari
E
E
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D005786:Gene Expression Regulation; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D008969:Molecular Sequence Data; D010306:Parotid Gland / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016315:Proto-Oncogene Proteins c-abl; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D014158:Transcription, Genetic
D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta; D016315:Proto-Oncogene Proteins c-abl; D007545:Isoproterenol