Binding of GAP to activated PDGF receptors.
The ras proto-oncogene products appear to relay intracellular signals via the Ras guanosine triphosphatase (GTPase) activator protein, GAP. In dog epithelial cells expressing human platelet-derived growth factor (PDGF) receptors, binding of PDGF caused approximately one-tenth of the total GAP molecules to complex with the receptor. Studies with mutant PDGF receptors showed that maximum association required both receptor kinase activity and phosphorylatable tyrosine residues at both the identified sites of receptor autophosphorylation.
Kazlauskas
A
A
Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
Ellis
C
C
Pawson
T
T
Cooper
J A
JA
D000818:Animals; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D020558:GTP Phosphohydrolases / Q000378:metabolism; D020690:GTPase-Activating Proteins; D006801:Humans; D015151:Immunoblotting; D015689:Oncogene Protein p21(ras) / Q000378:metabolism; D010449:Peptide Mapping; D010748:Phosphopeptides / Q000032:analysis; D010766:Phosphorylation; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D011233:Precipitin Tests; D011494:Protein Kinases / Q000032:analysis; D011506:Proteins / Q000378:metabolism*; D000090063:Proto-Oncogene Mas; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017479:Receptors, Platelet-Derived Growth Factor; D014443:Tyrosine / Q000378:metabolism; D020703:ras GTPase-Activating Proteins
D020690:GTPase-Activating Proteins; C000715470:MAS1 protein, human; D010748:Phosphopeptides; D010982:Platelet-Derived Growth Factor; D011506:Proteins; D000090063:Proto-Oncogene Mas; D011956:Receptors, Cell Surface; D020703:ras GTPase-Activating Proteins; D014443:Tyrosine; D011494:Protein Kinases; D017479:Receptors, Platelet-Derived Growth Factor; D020558:GTP Phosphohydrolases; D015689:Oncogene Protein p21(ras)