Guinea pig vas deferens contains sigma but not phencyclidine receptors.
Homogenates from guinea pig vas deferens were examined for possible existence of sigma receptor and phencyclidine (PCP) receptor using a radioligand binding assay. [3H](+)-SKF-10047 bound saturably in the homogenates to a single population of binding sites with a Kd of 311 and a Bmax of 1090 fmol/mg protein. IC50S of several representative sigma in displacing [3H](+)-SKF-10047 from this binding site were very much similar to the IC50S of those drugs in the brain sigma receptor binding assay. A correlation coefficient of 0.96 was obtained when IC50S from vas deferens were compared with those from the brain assay. Attempts to demonstrate PCP receptors in this tissue using [3H]TCP (N-[1-(2-thienyl)cyclohexyl]piperidine), however, yielded negative results. The results indicate that while guinea pig vas deferens contains sigma receptors, PCP receptors may be absent in this tissue.
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Neurochemistry Unit, National Institute on Drug Abuse, Baltimore, MD 21224.
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D000818:Animals; D001667:Binding, Competitive; D006168:Guinea Pigs; D008297:Male; D010620:Phenazocine / Q000031:analogs & derivatives / Q000378:metabolism; D010622:Phencyclidine / Q000031:analogs & derivatives / Q000378:metabolism; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D011957:Receptors, Opioid / Q000378:metabolism*; D017478:Receptors, Phencyclidine; D017480:Receptors, sigma; D014649:Vas Deferens / Q000378:metabolism*
D017981:Receptors, Neurotransmitter; D011957:Receptors, Opioid; D017478:Receptors, Phencyclidine; D017480:Receptors, sigma; C012134:SK&F 10047; C012058:tenocyclidine; D010620:Phenazocine; D010622:Phencyclidine