title: Identification of an amino acid sequence from the laminin A chain that stimulates metastasis and collagenase IV production.
abstract: Tumor cells attach, degrade, and migrate through basement membranes as they metastasize. Laminin, a major glycoprotein of basement membranes, promotes the metastatic activity of tumor cells by stimulating the attachment and migration of the cells and their secretion of collagenase IV. We have identified a synthetic peptide of 19 amino acids (Cys-Ser-Arg-Ala-Arg-Lys-Gln-Ala-Ala-Ser-Ile-Lys-Val-Ala-Val-Ser-Ala-Asp -Arg) from the sequence of the A chain of laminin that increases experimental metastases of the lungs by murine melanoma cells. The peptide is active when injected either intravenously or intraperitoneally. The peptide increased collagenase IV activity, a key enzyme in the breakdown of basement membranes, to the same extent as laminin. This peptide represents an active site on laminin for promotion of the metastatic phenotype and generates a probe for studying the regulation of malignant activities.
authors.affiliation: Laboratory of Developmental Biology and Anomalies, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892.
authors.forename: T
authors.initials: T
authors.lastname: Kanemoto
authors.forename: R
authors.initials: R
authors.lastname: Reich
authors.forename: L
authors.initials: L
authors.lastname: Royce
authors.forename: D
authors.initials: D
authors.lastname: Greatorex
authors.forename: S H
authors.initials: SH
authors.lastname: Adler
authors.forename: N
authors.initials: N
authors.lastname: Shiraishi
authors.forename: G R
authors.initials: GR
authors.lastname: Martin
authors.forename: Y
authors.initials: Y
authors.lastname: Yamada
authors.forename: H K
authors.initials: HK
authors.lastname: Kleinman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005354:Fibrosarcoma / Q000473:pathology*; D006801:Humans; D007797:Laminin / Q000494:pharmacology*; D046911:Macromolecular Substances; D008546:Melanoma, Experimental / Q000201:enzymology / Q000473:pathology*; D051379:Mice; D003012:Microbial Collagenase / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D010455:Peptides / Q000138:chemical synthesis / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000201:enzymology
chemical_list: D007797:Laminin; D046911:Macromolecular Substances; D010455:Peptides; D003012:Microbial Collagenase