[Autonomic replication of plasmids containing DNA fragments from rat liver alpha-polymerase complex].
We have cloned a number of plasmids pori, containing DNA fragments derived from rat liver alpha-polymerase complex. Colony hybridization identified 30 clones with homologies to origin SV40. Five clones of pori were examined in transfected V79 hamster cells. Plasmid DNA was isolated at time intervals after transfection and screened by the CfuI resistance assay or by the bromodeoxyuridine substitution. We have identified two plasmids pori22 and pori32 that can support plasmid replication in mammalian cells. Thus, at least two DNA fragments from rat liver alpha-polymerase complex act as origins of replication in a mammalian system. Five days after transfection of pori32 Hirt the supernatant contains the molecules of RNA which hybridize with pUC19. Our results indicated that pori32 has an origin of replication and promotor of transcription.
Timchenko
N A
NA
Shcherbakov
O G
OG
Krutiakov
V M
VM
Filatov
M V
MV
D000818:Animals; D015139:Blotting, Southern; D002461:Cell Line, Transformed; D006224:Cricetinae; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004257:DNA Polymerase II / Q000235:genetics*; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D008099:Liver / Q000201:enzymology*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D051381:Rats
D004247:DNA; D004257:DNA Polymerase II; D004262:DNA Restriction Enzymes
Avtonomnaia replikatsiia plazmid, soderzhashchikh fragmenty DNK iz alpha-polimeraznogo kompleksa pecheni krysy.