title: Glutaraldehyde effect on hemoglobin: evidence for an ion environment modification based on electron paramagnetic resonance and Mossbauer spectroscopies.
abstract: Glutaraldehyde is a widely used reagent for hemoglobin cross-linking in blood substitutes research. However, hemoglobin polymerization by glutaraldehyde involves modifications of its functional properties, such as oxygen affinity, redox potentials, and autoxidation kinetics. The aim of this article is to investigate, by electron paramagnetic resonance and Mossbauer spectroscopies, the changes that occur in the iron environment after glutaraldehyde cross-linking. Spectrometric studies were performed with native hemoglobin and hemoglobin cross-linked as soluble and insoluble polymers. Spectrometry data comparison with glutaraldehyde-modified hemoglobin functional properties allows to interpret from a structural point of view that glutaraldehyde action occurs as a decrease of the O--N(F8His) distance, an increase of the Fe--N(F8His) bond length, and the decrease of the distal-side steric hindrance.
authors.affiliation: Laboratoire de biophysique, Centre hospitalier universitaire Necker, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Chevalier
authors.forename: D
authors.initials: D
authors.lastname: Guillochon
authors.forename: N
authors.initials: N
authors.lastname: Nedjar
authors.forename: J M
authors.initials: JM
authors.lastname: Piot
authors.forename: M W
authors.initials: MW
authors.lastname: Vijayalakshmi
authors.forename: D
authors.initials: D
authors.lastname: Thomas
mesh_terms: D003432:Cross-Linking Reagents / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D005976:Glutaral / Q000494:pharmacology*; D006454:Hemoglobins / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D007501:Iron; D010108:Oxyhemoglobins / Q000378:metabolism / Q000648:ultrastructure; D011108:Polymers; D011487:Protein Conformation / Q000187:drug effects; D015204:Spectroscopy, Mossbauer
chemical_list: D003432:Cross-Linking Reagents; D006454:Hemoglobins; D010108:Oxyhemoglobins; D011108:Polymers; D007501:Iron; D005976:Glutaral