Induction of protective immunity in animals vaccinated with recombinant vaccinia viruses that express PreM and E glycoproteins of Japanese encephalitis virus.
A cDNA clone representing the genome of structural proteins of Japanese encephalitis virus (JEV) was inserted into the thymidine kinase gene of vaccinia virus strains LC16mO and WR under the control of a strong early-late promoter for the vaccinia virus 7.5-kilodalton polypeptide. Indirect immunofluorescence and fluorescence-activated flow cytometric analysis revealed that the recombinant vaccinia viruses expressed JEV E protein on the membrane surface, as well as in the cytoplasm, of recombinant-infected cells. In addition, the E protein expressed from the JEV recombinants reacted to nine different characteristic monoclonal antibodies, some of which have hemagglutination-inhibiting and JEV-neutralizing activities. Radioimmunoprecipitation analysis demonstrated that two major proteins expressed in recombinant-infected cells were processed and glycosylated as the authentic PreM and E glycoproteins of JEV. Inoculation of rabbits with the infectious recombinant vaccinia virus resulted in rapid production of antiserum specific for the PreM and E glycoproteins of JEV. This antiserum had both hemagglutination-inhibiting and virus-neutralizing activities against JEV. Furthermore, mice vaccinated with the recombinant also produced JEV-neutralizing antibodies and were resistant to challenge with JEV.
Yasuda
A
A
Biological Science Laboratory, Nippon Zeon Co. Ltd., Kanagawa, Japan.
Kimura-Kuroda
J
J
Ogimoto
M
M
Miyamoto
M
M
Sata
T
T
Sato
T
T
Takamura
C
C
Kurata
T
T
Kojima
A
A
Yasui
K
K
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D002460:Cell Line; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000276:immunology*; D004672:Encephalitis, Japanese / Q000276:immunology / Q000517:prevention & control*; D005260:Female; D005814:Genes, Viral; D006168:Guinea Pigs; D006801:Humans; D007114:Immunization; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010957:Plasmids; D011498:Protein Precursors / Q000235:genetics / Q000276:immunology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology; D015183:Restriction Mapping; D013937:Thymidine Kinase / Q000235:genetics; D014616:Vaccinia virus / Q000235:genetics; D014709:Vero Cells; D015678:Viral Structural Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D011498:Protein Precursors; D011994:Recombinant Proteins; D015678:Viral Structural Proteins; D014765:Viral Vaccines; D013937:Thymidine Kinase