[Increase of calcium ion level in the cytoplasm of indo 1-loaded HeLa cells under the action of histamine].
The regulation of free cytoplasmic calcium in HeLa cells was studied using a fluorescent calcium indicator into 1. It is found that addition of histamine caused substantial increase in calcium level. In calcium-free medium the increase was much smaller, implicating both intra- and extracellular calcium as sources of observed Ca2(+)-rise. Protein kinase C activator, phorbol myristate acetate, completely blocked the effect of histamine. Agents causing rise in cellular cAMP level did not change the Ca2(+)-rise.
Bochkov
V N
VN
Cheglakov
I B
IB
Ligum
D E
DE
Gavrilov
I Iu
IIu
Avdonin
P V
PV
D002118:Calcium / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism*; D005453:Fluorescence; D006367:HeLa Cells; D006632:Histamine / Q000494:pharmacology*; D006801:Humans; D007202:Indicators and Reagents; D008838:Microcomputers; D008954:Models, Biological; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007202:Indicators and Reagents; D006632:Histamine; D000242:Cyclic AMP; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium
Povyshenie urovnia ionov kal'tsiia v tsitoplazme kletok HeLa, zagruzhennykh indo l, pod vliianiem gistamina.