DNA-hybridization electron microscopy. Localization of five regions of 16 S rRNA on the surface of 30 S ribosomal subunits.
DNA-hybridization electron microscopy has been used to locate five regions of 16 S rRNA on the surface of 30 S ribosomal subunits. Biotinylated DNA probes that are complementary to selected regions of 16 S rRNA were hybridized to activated 30 S ribosomal subunits. These hybridized probes were reacted with avidin and localized by electron microscopy. The specificity of DNA binding was monitored with RNase H, which recognizes RNA-DNA hybrids and cleaves the RNA. Three of the five sequences examined were mapped on the platform. These sequences are 686-703, 714-733 and 787-803. Region 1492-1505 is mapped in the cleft and region 518-533 is at the neck on the side opposite the platform, respectively.
Oakes
M I
MI
Molecular Biology Institute, University of California, Los Angeles 90024.
Lake
J A
JA
D001483:Base Sequence; D015342:DNA Probes; D004722:Endoribonucleases; D004926:Escherichia coli / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods; D008961:Models, Structural; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D012335:RNA, Ribosomal / Q000648:ultrastructure*; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000648:ultrastructure*; D016914:Ribonuclease H; D012270:Ribosomes / Q000648:ultrastructure*
D015342:DNA Probes; D015345:Oligonucleotide Probes; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D004722:Endoribonucleases; D016914:Ribonuclease H