title: Chromosomal localization of the human myeloperoxidase gene by in situ hybridization using oligonucleotide probes.
abstract: Oligonucleotide probes have been used to map the myeloperoxidase (MPO) gene locus to chromosome bands 17q21-22. This is in agreement with results reported using conventional cDNA probes. No evidence for the existence of a second MPO gene locus was obtained. Six synthetic 72-base oligonucleotides, corresponding to different exon regions of the MPO gene, were tritium-labeled and used as in situ hybridization probes. Synthetic oligonucleotide probes offer a useful alternative to conventional DNA probes for gene mapping.
authors.affiliation: Department of Pathology, Emory University School of Medicine, Atlanta, Georgia.
authors.forename: S R
authors.initials: SR
authors.lastname: Zaki
authors.forename: G E
authors.initials: GE
authors.lastname: Austin
authors.forename: W C
authors.initials: WC
authors.lastname: Chan
authors.forename: A L
authors.initials: AL
authors.lastname: Conaty
authors.forename: S
authors.initials: S
authors.lastname: Trusler
authors.forename: S
authors.initials: S
authors.lastname: Trappier
authors.forename: R B
authors.initials: RB
authors.lastname: Lindsey
authors.forename: D C
authors.initials: DC
authors.lastname: Swan
mesh_terms: D002871:Chromosome Banding; D002886:Chromosomes, Human, Pair 17; D015342:DNA Probes; D006801:Humans; D009693:Nucleic Acid Hybridization; D009195:Peroxidase / Q000235:genetics*
chemical_list: D015342:DNA Probes; D009195:Peroxidase