title: Transcription initiated by RNA polymerase II and purified transcription factors from liver. A complex set of promoter sequences governs formation of the initial complex.
abstract: Using a transcription system derived from rat liver, we have investigated the function of the TATA-box and cap site/initiator regions in assembly of stable intermediates on the pathway leading to formation of a functional RNA polymerase II preinitiation complex. Our findings argue that neither the TATA-box region nor the cap site/initiator region functions independently in the binding of transcription factors during formation of these intermediates, but, rather, the two sequence elements function cooperatively to direct assembly of a stable preinitiation complex at the promoter.
authors.affiliation: Department of Chemistry, University of Texas, Austin 78712-1096.
authors.forename: J W
authors.initials: JW
authors.lastname: Conaway
authors.forename: E
authors.initials: E
authors.lastname: Travis
authors.forename: R C
authors.initials: RC
authors.lastname: Conaway
mesh_terms: D000818:Animals; D001483:Base Sequence; D008297:Male; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013698:Templates, Genetic; D014157:Transcription Factors / Q000302:isolation & purification / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D014157:Transcription Factors; D012319:RNA Polymerase II