title: Ectopic expression from the Deformed gene triggers a dominant defect in Drosophila adult head development.
abstract: Drosophila adults carrying a dominant allele of the Deformed locus (DfdD) have a mutant phenotype in which lower eye and orbital structures of the adult head are eliminated. The molecular defect responsible for this dominant mutation is a large, transposon-flanked, fragment of DNA inserted downstream of the Deformed (Dfd) transcription unit. The downstream transposon-flanked insert causes the inappropriate activation of Dfd gene expression in a subset of the cells of the eye imaginal disc, resulting in their abnormal development, and in the loss of lower eye structures from the adult head. A chromosome selected for its reversion of the dominant phenotype is missing a portion of the transposon-flanked insert and reverts to a normal expression pattern of Dfd in the eye-antennal disc. Additional evidence that the dominant head phenotype is caused by ectopic expression of Dfd in the eye disc derives from experiments showing that a DfdD-like phenotype can be induced by ectopic expression of a Dfd gene under heat shock promoter control.
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511.
authors.forename: R
authors.initials: R
authors.lastname: Chadwick
authors.forename: B
authors.initials: B
authors.lastname: Jones
authors.forename: T
authors.initials: T
authors.lastname: Jack
authors.forename: W
authors.initials: W
authors.lastname: McGinnis
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D004247:DNA / Q000032:analysis; D004251:DNA Transposable Elements / Q000502:physiology; D004330:Drosophila / Q000235:genetics*; D005786:Gene Expression Regulation; D006257:Head / Q000033:anatomy & histology / Q000196:embryology*; D010641:Phenotype; D015183:Restriction Mapping
chemical_list: D004251:DNA Transposable Elements; D004247:DNA