In vivo bioluminescence: a cellular reporter for research and industry.
The detection of specific bacterial pathogens, indicator microorganisms and antimicrobial substances, and the recovery of microorganisms from sub-lethal injury, are all aspects of importance to industry which are currently being targeted using in vivo bioluminescence. In all instances, a key requirement for the application of bioluminescence is the establishment of a strict correlation between in vivo bioluminescence and cell viability, as determined by colony counting on agar plates. Comparative studies for biocides (phenol, chlorhexidine diacetate, phenol thioether), for a virucide (hypochlorite) and for cellular recovery of S. typhimurium from sub-lethal injury, all indicate that such a correlation is valid. Furthermore, real-time measurements of in vivo bioluminescence reveal a major population of bacterial cells that retain functional intracellular biochemistry, but are defective in their ability to replicate post of freeze injury.
Jassim
S A
SA
Department of Applied Biochemistry & Food Science, Faculty of Agricultural & Food Sciences, University of Nottingham, Loughborough, Leicestershire, UK.
Ellison
A
A
Denyer
S P
SP
Stewart
G S
GS
D010582:Bacteriophage lambda / Q000187:drug effects* / Q000254:growth & development; D002710:Chlorhexidine / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D006997:Hypochlorous Acid / Q000494:pharmacology; D007221:Industry; D007700:Kinetics; D008156:Luciferases / Q000235:genetics* / Q000378:metabolism; D008163:Luminescent Measurements; D008826:Microbial Sensitivity Tests / Q000379:methods; D010636:Phenols / Q000494:pharmacology; D010641:Phenotype; D010957:Plasmids; D012107:Research Design; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development
D010636:Phenols; D006997:Hypochlorous Acid; D008156:Luciferases; D002710:Chlorhexidine