An improved purification procedure for conjugated bile acids using octadecyl-bonded silica cartridges.
A rapid procedure for the extraction of conjugated bile acids from human fluids using pre-packed octadecyl-bonded silica cartridges is described. The method was compared with the other procedures reported in the literature and was found to produce a higher degree of sample purification and to achieve satisfactory accuracy and reproducibility. The present procedure is applicable to the high-performance liquid chromatographic assay of bile acid conjugates in human bile, gastric juice, serum and urine.
Scalia
S
S
Dipartimento di Scienze Farmaceutiche, Università di Ferrara.
Guarneri
M
M
D001646:Bile / Q000032:analysis; D001647:Bile Acids and Salts / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D004386:Duodenum / Q000032:analysis; D006801:Humans; D007202:Indicators and Reagents; D012822:Silicon Dioxide; D013270:Stomach / Q000032:analysis
D001647:Bile Acids and Salts; D007202:Indicators and Reagents; D012822:Silicon Dioxide