Third place--Resident Basic Science Award 1990. Interleukin 1 causing bone destruction in middle ear cholesteatoma.
We previously reported the localization of interleukin 1 in the epithelial layer of human cholesteatomas. On the basis of other studies that showed interleukin 1 can stimulate fibroblasts and macrophages to produce collagenases and prostaglandins, we then proposed that interleukin 1 may play an important role in cholesteatoma-related bone resorption, also. Our immunocytochemical study involving more human cholesteatoma samples revealed the presence of interleukin 1 in bone cells and monocytes in the region of active bone destruction. In the present study, the effect of interleukin 1 on these cells found at the bone resorption site was examined. By radioimmunoassay, interleukin 1 was shown to stimulate the production of prostaglandin E2 by osteoblasts in vitro. Interleukin 1 also promoted the migration and multinucleation of bone marrow-derived monocytes. These osteoclast-like cells formed from monocytes contained tartrate-resistant acid phosphatase, and caused the resorption of the devitalized bone in vitro. Above findings suggest that interleukin 1 could cause the bone destruction in cholesteatomas, not only by stimulating the local bone cells, but also by recruiting monocytes for osteoclastic bone resorption.
Ahn
J M
JM
Department of Otolaryngology, Columbia University, New York, NY 10032.
Huang
C C
CC
Abramson
M
M
D000047:Academies and Institutes; D000818:Animals; D001363:Awards and Prizes; D001862:Bone Resorption / Q000503:physiopathology*; D002478:Cells, Cultured / Q000187:drug effects; D002781:Cholesteatoma / Q000503:physiopathology*; D015232:Dinoprostone / Q000096:biosynthesis; D006801:Humans; D007124:Immunoenzyme Techniques; D007378:Interleukins / Q000494:pharmacology / Q000502:physiology*; D003012:Microbial Collagenase / Q000096:biosynthesis; D009000:Monocytes / Q000187:drug effects / Q000201:enzymology; D010033:Otitis Media / Q000503:physiopathology*; D010036:Otolaryngology; D011863:Radioimmunoassay; D051381:Rats; D014481:United States
D007378:Interleukins; D003012:Microbial Collagenase; D015232:Dinoprostone