Beta-cyclodextrin bonded silica for direct injection analysis of drug enantiomers in serum by liquid chromatography.
A new beta-cyclodextrin (CD) bonded silica has been designed for direct injection analysis of drug enantiomers in serum for liquid chromatography. The new beta-CD bonded silica is synthesized by three steps: introduction of a 3-glycidoxypropyl phase, introduction of a beta-CD carbamate-bonded phase, and hydrolysis of the oxirane ring to a diol phase. Although the recovery of serum proteins from only a beta-CD bonded silica (having no diol phase) was poor, they were completely recovered from a mixed functional silica having beta-CD and diol phases. Direct injection analysis of drug enantiomers in serum can be attained with the mixed functional silica support over the eluent pH range of 3-7. The recovery of racemic drugs from serum was almost 100%.
Haginaka
J
J
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.
Wakai
J
J
D002853:Chromatography, Liquid; D003505:Cyclodextrins / Q000032:analysis*; D003912:Dextrins / Q000032:analysis*; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000302:isolation & purification*; D012822:Silicon Dioxide / Q000032:analysis*; D013213:Starch / Q000032:analysis*; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D003912:Dextrins; D004364:Pharmaceutical Preparations; D047392:beta-Cyclodextrins; D012822:Silicon Dioxide; D013213:Starch; C031215:betadex