title: Cloning of the genes encoding urease from Proteus vulgaris and sequencing of the structural genes.
abstract: A fragment of chromosomal DNA from proteus vulgaris encoding urease was cloned and expressed in Escherichia coli. A 3 kbp region was sequenced and revealed three open reading frames with homology to jack bean (Canavalia ensiformis) urease. The smallest protein (11 kDa) was homologous to the N-terminus of the plant enzyme and the largest polypeptide (61 kDa) corresponded to the C-terminus. The large protein contained conserved regions and a cysteine residue which is known to be catalytically important in the plant enzyme. A protein of 12 kDa showed homology to residues 132 to 237 of jack bean urease.
authors.affiliation: Fachrichtung Mikrobiologie der Universität des Saarlandes, F.R.G.
authors.forename: G
authors.initials: G
authors.lastname: Mörsdorf
authors.forename: H
authors.initials: H
authors.lastname: Kaltwasser
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011514:Proteus vulgaris / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014510:Urease / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D014510:Urease