Induction of type II iodothyronine 5'-deiodinase and mitochondrial uncoupling protein in brown adipocytes differentiated in cell culture.
Brown adipocytes differentiated in primary cell culture were found to contain a type II iodothyronine 5'-deiodinase (5'D). Incubation of confluent cells with norepinephrine or dibutyryl-cAMP caused up to 17-fold increase in 5'D activity with a maximum after 8 h. Activation of 5'D required mRNA and protein synthesis and was accompanied by parallel, up to 5.8-fold increase in the amount of mitochondrial uncoupling protein with a maximum after 24 h. Analysis of adrenergic stimulation of 5'D suggested predominant involvement of the beta-receptors and increased intracellular cAMP levels, while the contribution of alpha 1-receptors was small.
Houstĕk
J
J
Institute of Physiology, Czechoslovak Academy of Sciences, Prague.
Pavelka
S
S
Baudysová
M
M
Kopecký
J
J
D002001:Adipose Tissue, Brown / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002352:Carrier Proteins; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004790:Enzyme Induction; D007453:Iodide Peroxidase / Q000096:biosynthesis*; D007473:Ion Channels; D007545:Isoproterenol / Q000494:pharmacology; D007700:Kinetics; D008565:Membrane Proteins / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins; D009638:Norepinephrine / Q000494:pharmacology; D010656:Phenylephrine / Q000494:pharmacology; D011224:Prazosin / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D000071256:Uncoupling Protein 1
D002352:Carrier Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D000071256:Uncoupling Protein 1; D003609:Dactinomycin; D005576:Colforsin; D010656:Phenylephrine; D003994:Bucladesine; D003513:Cycloheximide; D011433:Propranolol; D007453:Iodide Peroxidase; D007545:Isoproterenol; D009638:Norepinephrine; D011224:Prazosin