title: Oriented immobilization of periodate-oxidized monoclonal antibodies on amino and hydrazide derivatives of Eupergit C.
abstract: Amino and hydrazyno derivatives of Eupergit C were prepared by reaction of the beads with hexamethylene diamine (HMD) and adipic acid dihydrazide (ADH), respectively. Monoclonal antibodies (mAbs) against carboxypeptidase A (CPA) and horse radish peroxidase (HRP) were prepared, and those that did not inhibit the respective enzymatic activities were selected. The carbohydrate moieties of these antibodies were oxidized by reaction with sodium periodate and then coupled onto the modified beads. The oxidation and coupling reactions were optimized to achieve highly active matrix-conjugated antibodies. Thus, antibody-matrix conjugates that possessed antigen-binding activities close to the theoretical value of 2 mol antigen bound/mol immobilized antibody were obtained.
authors.affiliation: Department of Biotechnology, Faculty of Life Sciences, Tel-Aviv University, Israel.
authors.forename: G
authors.initials: G
authors.lastname: Fleminger
authors.forename: E
authors.initials: E
authors.lastname: Hadas
authors.forename: T
authors.initials: T
authors.lastname: Wolf
authors.forename: B
authors.initials: B
authors.lastname: Solomon
mesh_terms: D000180:Acrylic Resins; D000272:Adipates; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002268:Carboxypeptidases / Q000276:immunology; D043422:Carboxypeptidases A; D003959:Diamines; D006735:Horseradish Peroxidase / Q000276:immunology; D007074:Immunoglobulin G; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D010084:Oxidation-Reduction; D010504:Periodic Acid
chemical_list: D000180:Acrylic Resins; D000272:Adipates; D000911:Antibodies, Monoclonal; D003959:Diamines; D007074:Immunoglobulin G; D010504:Periodic Acid; C061522:Eupergit C1Z; C009288:metaperiodate; D006735:Horseradish Peroxidase; D002268:Carboxypeptidases; D043422:Carboxypeptidases A; C010011:adipic dihydrazide; C007297:1,6-diaminohexane