title: Infectious bursal disease virus variant from commercial Leghorn pullets.
abstract: An infectious bursal disease virus (IBDV) was isolated from 39-to-43-day-old commercial leghorn pullets suspected of having infectious bursal disease (IBD). These chickens had been vaccinated with a commercial live IBDV vaccine at 28 and 35 days of age. An isolate designated IN was recovered using specific-pathogen-free (SPF) chickens and the BGM-70 established cell line. Experimental studies using SPF chickens vaccinated with either inactivated vaccines made from the vaccine strain used in the problem flock or a standard-type vaccine indicated no protection against the IN isolate. However, two variants and another standard-type vaccine induced protection against the IN isolate. Cross-neutralization tests indicated that the IN isolate differed antigenically from commercial vaccine strains and was related to the variant IBDV strains recently isolated from broilers. To our knowledge, this is the first report of a variant IBDV recovered from commercial layer chickens in the United States.
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.
authors.forename: N M
authors.initials: NM
authors.lastname: Ismail
authors.forename: Y M
authors.initials: YM
authors.lastname: Saif
authors.forename: W L
authors.initials: WL
authors.lastname: Wigle
authors.forename: G B
authors.initials: GB
authors.lastname: Havenstein
authors.forename: C
authors.initials: C
authors.lastname: Jackson
mesh_terms: D000818:Animals; D000940:Antigenic Variation; D002060:Bursa of Fabricius / Q000473:pathology; D002460:Cell Line; D002645:Chickens; D003429:Cross Reactions; D005260:Female; D007243:Infectious bursal disease virus / Q000276:immunology* / Q000302:isolation & purification; D009500:Neutralization Tests; D011201:Poultry Diseases / Q000382:microbiology*; D012087:Reoviridae / Q000276:immunology*; D012088:Reoviridae Infections / Q000382:microbiology / Q000662:veterinary*; D013047:Specific Pathogen-Free Organisms; D014611:Vaccination / Q000662:veterinary