Modulation of arachidonic acid metabolism by bovine alveolar macrophages exposed to interferons and lipopolysaccharide.
Stimulation of bovine alveolar macrophages with calcium ionophore A23187 resulted in marked production of leukotriene (LT)B4 and a lesser increase in thromboxane (TX)B2, whereas opsonized zymosan (OPZ) resulted in production of TXB2 and relatively small increases in LTB4 and prostaglandin (PG)F2 alpha. Alveolar macrophages incubated with recombinant bovine interferon-gamma or lipopolysaccharide, and subsequently stimulated with A23187 or OPZ, had altered arachidonic acid metabolism, producing markedly increased amounts of TXB2 and PGF2 alpha, and slightly increased LTB4. Incubation of alveolar macrophages with lipopolysaccharide had a more profound effect on the increased amounts of TXB2 and PGF2 alpha, observed in response to stimulation with A23187 or OPZ, than did incubation with interferon-gamma. Alveolar macrophages incubated with recombinant bovine interferon-alpha 1-1 also produced slightly increased amounts of LTB4 when stimulated with A23187 or OPZ. Altered arachidonic acid metabolism by alveolar macrophages exposed to interferons and lipopolysaccharide may contribute to the development of pulmonary inflammation, such as in the early stages of bacterial pneumonia following viral infections that induce interferon production.
O'Sullivan
M G
MG
Department of Microbiology, College of Veterinary Medicine, North Carolina State University, Raleigh 27606.
Fleisher
L N
LN
Olson
N C
NC
MacLachlan
N J
NJ
Brown
T T
TT
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002417:Cattle; D015237:Dinoprost / Q000096:biosynthesis / Q000737:chemistry; D007370:Interferon Type I / Q000378:metabolism* / Q000494:pharmacology; D007975:Leukotriene B4 / Q000032:analysis / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000378:metabolism* / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D011994:Recombinant Proteins; D013929:Thromboxane B2 / Q000032:analysis / Q000096:biosynthesis; D015054:Zymosan / Q000494:pharmacology
D001095:Arachidonic Acids; D007370:Interferon Type I; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D007975:Leukotriene B4; D016718:Arachidonic Acid; D000001:Calcimycin; D013929:Thromboxane B2; D015054:Zymosan; D015237:Dinoprost