Free and small peptide-bound [14C]hydroxyproline synthesis in vitro in ethanol-induced hepatic injury in the rat liver.
To clarify the significance of free and small peptide-bound hydroxyproline synthesis in ethanol-induced liver injury, we measured the in vitro synthesis of [14C]hydroxyproline in the 67% ethanol-soluble fraction in rat liver slices, together with hepatic protein-bound [14C]hydroxyproline synthesis. The synthesis of free and small peptide-bound [14C]hydroxyproline was 11.1 +/- 2.0 dpm x 10(-4)/g liver/3 hr and the synthesis of protein-bound [14C]hydroxyproline was 10.1 +/- 3.3 dpm x 10(-4)/g liver/3 hr in control rat liver. In the ethanol-fed rat liver, the synthesis of free and small peptide-bound [14C]hydroxyproline significantly increased 1.5-fold and the synthesis of protein-bound [14C]hydroxyproline significantly increased 1.6-fold, while the hepatic collagen content did not change. There was a significant correlation between free and small peptide-bound [14C]hydroxyproline synthesis and protein-bound [14C]hydroxyproline synthesis. These results suggest that free and small peptide-bound hydroxyproline synthesis plays an important role in regulating the content of hepatic collagens.
Koda
M
M
Second Department of Internal Medicine, Tottori University, School of Medicine, Yonago, Japan.
Murawaki
Y
Y
Hirayama
C
C
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D000431:Ethanol / Q000633:toxicity*; D006909:Hydroxyproline / Q000096:biosynthesis* / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D003012:Microbial Collagenase / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D010455:Peptides / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains
D010455:Peptides; D000431:Ethanol; D003012:Microbial Collagenase; D006909:Hydroxyproline