title: A species-specific sperm-activating peptide from the egg jelly of the sea urchin Diadema setosum.
abstract: 1. A species-specific sperm-activating peptide was isolated from the egg jelly of the sea urchin Diadema setosum and the amino acid sequence was determined as follows: (formula; see text). 2. The peptide caused significant increases of respiration rates and cyclic nucleotide concentrations in D. setosum spermatozoa as low as 10(-9) M. 3. The addition of the peptide to D. setosum spermatozoa resulted in the appearance of a newly stained protein (mol. wt 128,000) on sodium dodecyl sulfate-polyacrylamide gels.
authors.affiliation: Noto Marine Laboratory, Kanazawa University, Ishikawa, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yoshino
authors.forename: M
authors.initials: M
authors.lastname: Kurita
authors.forename: M
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: K
authors.initials: K
authors.lastname: Nomura
authors.forename: T
authors.initials: T
authors.lastname: Takao
authors.forename: Y
authors.initials: Y
authors.lastname: Shimonishi
authors.forename: N
authors.initials: N
authors.lastname: Suzuki
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000242:Cyclic AMP / Q000032:analysis; D006152:Cyclic GMP / Q000032:analysis; D008297:Male; D008969:Molecular Sequence Data; D010063:Ovum / Q000032:analysis; D010101:Oxygen Consumption; D010456:Peptides, Cyclic / Q000302:isolation & purification; D012617:Sea Urchins / Q000032:analysis*; D013045:Species Specificity; D013094:Spermatozoa / Q000502:physiology
chemical_list: D000596:Amino Acids; D010456:Peptides, Cyclic; C063869:sperm-activating peptide IV, Diadema setosum; D000242:Cyclic AMP; D006152:Cyclic GMP