title: Isolation of very virulent Marek's disease viruses from vaccinated chickens in Australia.
abstract: Very virulent Marek's disease viruses (vvMDV), defined as isolates against which the herpesvirus of turkey (HVT) vaccine provide poor protection, have been isolated from poultry flocks in both the United States and Europe. Twenty-one samples from vaccinated Australian flocks, experiencing problems with excessive Marek's disease (MD), were tested for the presence of transmissible MD viruses (MDV). Of the 16 samples which contained a transmissible agent, 14 were pathogenic in chickens, based on the development of MD lesions or depression of the bursa/body weight ratio. Of the pathogenic isolates which have been successfully typed 10 were serotype 1, and one was serotype 2 MDV. Pathogenicity of isolates varied. Several isolates caused tumours in 20-30% of both vaccinated and unvaccinated chickens. Two isolates, MPF6 and MPF23, caused tumours in more than 50% of chickens. When MPF6 and MPF23 were tested in vaccine trials bivalent vaccine gave no better protection against development of MD lesions than a monovalent vaccine. Isolate MPF23 was so pathogenic that lesions were produced in all chickens, regardless of the vaccine protocol used. Therefore vvMDV have been isolated in Australia, and unlike the vaccines tested overseas, bivalent Australian vaccines do not appear to provide greater protection against these vvMDV.
authors.affiliation: National Biological Standards Laboratory, Parkville, Victoria.
authors.forename: J L
authors.initials: JL
authors.lastname: McKimm-Breschkin
authors.forename: J T
authors.initials: JT
authors.lastname: Faragher
authors.forename: J
authors.initials: J
authors.lastname: Withell
authors.forename: W M
authors.initials: WM
authors.lastname: Forsyth
mesh_terms: D000818:Animals; D001315:Australia; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D020022:Genetic Predisposition to Disease; D006564:Herpesviridae / Q000276:immunology; D008381:Herpesvirus 2, Gallid / Q000145:classification / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity*; D008380:Marek Disease / Q000382:microbiology* / Q000517:prevention & control; D012703:Serotyping; D013047:Specific Pathogen-Free Organisms; D014611:Vaccination / Q000662:veterinary; D014765:Viral Vaccines; D014774:Virulence
chemical_list: D014765:Viral Vaccines