Enhancement of topoisomerase I-mediated unwinding of supercoiled DNA by the radioprotector WR-33278.
The radioprotector WR-33278, the disulfide of WR-1065 (N-(2-mercaptoethyl)-1,3-diaminopropane), is shown to stimulate eukaryotic topoisomerase I unwinding of negatively supercoiled DNA. This observation suggests the possibility that some protection may be conferred to DNA either by a decrease in its supercoiled state or by altering directly other enzymatic processes. This is the first report of a radioprotective compound stimulating an enzyme involved in DNA structure and synthesis.
Holwitt
E A
EA
Radiation Biochemistry Department, Armed Forces Radiobiology Research Institute, Bethesda, Maryland 20814-5145.
Koda
E
E
Swenberg
C E
CE
D004264:DNA Topoisomerases, Type I / Q000494:pharmacology*; D004278:DNA, Superhelical / Q000187:drug effects*; D004357:Drug Synergism; D008624:Mercaptoethylamines / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*
D004278:DNA, Superhelical; D008624:Mercaptoethylamines; D011837:Radiation-Protective Agents; D004264:DNA Topoisomerases, Type I; C045611:WR 33278