Quantitative determination of lymphocyte ACTH1-39.
A method was developed for quantitative measurement of ACTH1-39, produced by human lymphocytes. It was shown that pH adjustment to 2 was essential for processing of the precursor molecule proopiomelanocortin (POMC). Linearity between time of incubation and ACTH production was shown. The existence of specific endopeptidases in lymphocytes for processing of the POMC molecule remains doubtful.
Ferreira
J A
JA
Department of Chemical Pathology, University of Stellenbosch, Tygerberg Hospital, Republic of South Africa.
Carstens
M E
ME
Taljaard
J J
JJ
D000324:Adrenocorticotropic Hormone / Q000032:analysis*; D000328:Adult; D002478:Cells, Cultured; D006801:Humans; D008214:Lymphocytes / Q000032:analysis* / Q000187:drug effects; D010434:Pepsin A / Q000494:pharmacology; D011333:Pro-Opiomelanocortin / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism
D011498:Protein Precursors; D011333:Pro-Opiomelanocortin; D000324:Adrenocorticotropic Hormone; D010434:Pepsin A