Detection of caprine arthritis-encephalitis- and maedi-visna viruses using the polymerase chain reaction.
The polymerase chain reaction (PCR) was used to demonstrate proviral DNA of lentiviruses of small ruminants in cultured cells. Primers for the Taq polymerase were selected in the GAG gene of Icelandic maedi-visna virus and POL gene of caprine arthritis-encephalitis (CAE) virus. Using PCR, proviral DNA of CAE virus was detected at 1 day post infection, 4 days before viral protein could be demonstrated using a sensitive immunoblotting protocol and 6 days before the appearance of syncytia. Primers derived from the published sequence of CAE virus successfully primed for the synthesis of homologous virus and Icelandic maedi-visna virus but not for maedi-visna virus isolated in The Netherlands. In contrast, primers derived from the GAG region of Icelandic maedi-visna virus allowed the amplification of DNA of homologous virus, maedi-visna virus isolated in The Netherlands as well as CAE virus.
Zanoni
R
R
Institute of Veterinary Virology, University of Bern, Switzerland.
Pauli
U
U
Peterhans
E
E
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis; D015750:Genes, gag; D015751:Genes, pol; D015151:Immunoblotting; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification; D012190:Retroviridae / Q000235:genetics / Q000302:isolation & purification*; D012756:Sheep; D014790:Visna-maedi virus / Q000235:genetics / Q000302:isolation & purification*
D004279:DNA, Viral