Endothelin/sarafotoxin receptor induced phosphoinositide turnover: effects of pertussis and cholera toxins and of phorbol ester.
The induction of phosphoinositide hydrolysis (PI) by endothelin/sarafotoxin (ET/SRTX) receptors in rat heart myocytes was investigated by the use of bacterial toxins as well as a phorbol ester. Both pertussis- and choleratoxin enhanced the stimulation of PI hydrolysis. Phorbol ester treatment of the myocytes for short periods distinguished between two types of PI-hydrolysis, the one induced by endothelins and the other by sarafotoxins. The possible mediation of G-protein (s) in the induction by ET/SRTX receptors of PI-hydrolysis is discussed.
Galron
R
R
Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.
Bdolah
A
A
Kloog
Y
Y
Sokolovsky
M
M
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000494:pharmacology*; D016232:Endothelins / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects; D007294:Inositol / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism*; D051381:Rats; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000502:physiology*; D011950:Receptors, Cholinergic / Q000187:drug effects / Q000502:physiology*; D017466:Receptors, Endothelin; D018000:Receptors, Peptide; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014662:Vasoconstrictor Agents / Q000494:pharmacology*; D014757:Viper Venoms / Q000494:pharmacology*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D016232:Endothelins; D010716:Phosphatidylinositols; D011956:Receptors, Cell Surface; D011950:Receptors, Cholinergic; D017466:Receptors, Endothelin; D018000:Receptors, Peptide; D014662:Vasoconstrictor Agents; D014757:Viper Venoms; D010566:Virulence Factors, Bordetella; C058085:sarafotoxin receptor; C057354:sarafotoxins s6; D007294:Inositol; D002772:Cholera Toxin; D013755:Tetradecanoylphorbol Acetate