Distribution of progestin-binding cells in estrogen-treated and untreated neonatal mouse uterus and oviduct: autoradiographic study with [125I]progestin.
Progestin-binding sites in uteri and oviducts of estrogen-treated and untreated 8-day-old mice were studied by thaw-mount autoradiography with [125I]progestin. In the untreated uteri, nuclear concentration of radiolabelled progestin was observed in all tissues of the uterus, with strongest nuclear labelling in luminal and glandular epithelia and in stroma. In the estrogen-treated uteri, the degree of labelling was markedly augmented in stroma and muscle, but much reduced in the luminal and glandular epithelia, compared to untreated uteri. In untreated oviducts, nuclear labelling was observed in stroma and muscle in all regions and in epithelium in the isthmic and uterine regions. The epithelium in infundibular and ampullar regions was only scarcely labelled. The estrogen-treatment augmented the labelling in stroma and muscle of the oviduct as in the uterus, but the labelling in epithelium was not affected. These results indicate that estrogen-treatment induces progesterone receptor differentially among tissue compartments both in the uterus and oviduct.
Murakami
R
R
Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599.
Shughrue
P J
PJ
Stumpf
W E
WE
Elger
W
W
Schulze
P E
PE
D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology; D004966:Estrogens, Conjugated (USP) / Q000494:pharmacology*; D005187:Fallopian Tubes / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D051379:Mice; D008813:Mice, Inbred ICR; D011372:Progestins / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism*; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
D004966:Estrogens, Conjugated (USP); D011372:Progestins; D011980:Receptors, Progesterone; D004958:Estradiol