The selenite-exchangeable metabolic pool in humans: a new concept for the assessment of selenium status.
An in vivo isotope-dilution approach is considered for assessment of selenium status in human subjects. The approach depends upon the dilution of a single dose of the stable isotope 74SeO3(2-) in the selenite-exchangeable metabolic pool. Data from six metabolic protocols, conducted with healthy North American males, are presented in order to analyze characteristics of this pool. Pool size (WSe-EMP) correlated positively with daily selenium intake in subjects consuming diets of known and variable selenium content. When subjects were given a selenium-adequate or -restricted diet for 30 d, WSe-EMP,7d decreased from 4.49 +/- 0.28 to 3.76 +/- 0.22 mg (p less than 0.05). The corresponding 24-h urinary selenium concentration dropped from 0.556 +/- 0.035 to 0.341 +/- 0.058 mumol/d (means +/- 1 SEM). Route of administration (iv vs po) had no apparent effect on WSe-EMP. In subjects of similar selenium status, the WSe-EMP was reproducible within the expected uncertainties of the method. This approach may be suitable for assessment of selenium status for a wide range of chronic intakes.
Janghorbani
M
M
Department of Medicine, University of Chicago, IL 60637.
Martin
R F
RF
Kasper
L J
LJ
Sun
X F
XF
Young
V R
VR
D000328:Adult; D004032:Diet; D006801:Humans; D007201:Indicator Dilution Techniques; D007275:Injections, Intravenous; D007554:Isotopes; D008297:Male; D008433:Mathematics; D008954:Models, Biological; D009501:Neutron Activation Analysis; D015596:Nutrition Assessment; D009752:Nutritional Status; D020887:Selenious Acid; D012643:Selenium / Q000008:administration & dosage / Q000172:deficiency / Q000378:metabolism*; D013997:Time Factors
D007554:Isotopes; D020887:Selenious Acid; D012643:Selenium