Localized transposon Tn5 mutagenesis of the photosynthetic gene cluster of Rhodobacter sphaeroides.
Four genes essential for bacteriochlorophyll biosynthesis were known to be encoded within a 45 kb region of the Rhodobacter sphaeroides genome, the boundaries of which are defined by puh and puf genes for reaction-centre and light-harvesting LH1 complexes. The cluster is represented by eight overlapping inserts cloned in the mobilizable vector pSUP202. We have used localized transposon Tn5 mutagenesis to characterize this cluster further; a total of 87 independent insertions were generated which identify nine genes for bacteriochlorophyll biosynthesis, six for carotenoid biosynthesis, and puhA encoding the reaction-centre H subunit. This work provides an essential framework for a detailed study of the structure and expression of genes for photosynthesis in this bacterium.
Coomber
S A
SA
Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, UK.
Chaudhri
M
M
Connor
A
A
Britton
G
G
Hunter
C N
CN
D001429:Bacteriochlorophylls / Q000235:genetics; D002338:Carotenoids / Q000235:genetics; D002874:Chromosome Mapping; D004251:DNA Transposable Elements; D045342:Light-Harvesting Protein Complexes; D005810:Multigene Family; D009154:Mutation; D010641:Phenotype; D010788:Photosynthesis / Q000235:genetics*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics; D012242:Rhodobacter sphaeroides / Q000235:genetics*; D013053:Spectrophotometry
D001429:Bacteriochlorophylls; D004251:DNA Transposable Elements; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D002338:Carotenoids