Alpha subunit variants of the human glycine receptor: primary structures, functional expression and chromosomal localization of the corresponding genes.
Two cDNAs encoding variants (alpha 1 and alpha 2) of the strychnine binding subunit of the inhibitory glycine receptor (GlyR) were isolated from a human fetal brain cDNA library. The predicted amino acid sequences exhibit approximately 99% and approximately 76% identity to the previously characterized rat 48 kd polypeptide. Heterologous expression of the human alpha 1 and alpha 2 subunits in Xenopus oocytes resulted in the formation of glycine-gated strychnine-sensitive chloride channels, indicating that both polypeptides can form functional GlyRs. Using a panel of rodent-human hybrid cell lines, the gene encoding alpha 2 was mapped to the short arm (Xp21.2-p22.1) of the human X chromosome. In contrast, the alpha 1 subunit gene is autosomally located. These data indicate molecular heterogeneity of the human GlyR at the level of alpha subunit genes.
Grenningloh
G
G
ZMBH, University of Heidelberg, FRG.
Schmieden
V
V
Schofield
P R
PR
Seeburg
P H
PH
Siddique
T
T
Mohandas
T K
TK
Becker
C M
CM
Betz
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D015870:Gene Expression; D014644:Genetic Variation; D005998:Glycine / Q000378:metabolism*; D006801:Humans; D006822:Hybrid Cells / Q000378:metabolism; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D051381:Rats; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014960:X Chromosome; D014981:Xenopus
D046911:Macromolecular Substances; D015345:Oligonucleotide Probes; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter; D004247:DNA; D005998:Glycine