Immunohistochemical characterization of rat thymic non-lymphoid cells. II. Macrophages and granulocytes defined by monoclonal antibodies.
A panel of monoclonal antibodies (mAb) raised to antigens of rat thymic non-lymphoid cells (predominantly macrophages and granulocytes) was immunohistochemically characterized. Based on their staining patterns on cryostat thymic sections and double labellings using acid phosphatase activity, anti-cytokeratin mAb to exclude binding to epithelium or ED1 and ED2 mAb, specific for rat macrophages, antibodies were subdivided into four groups: (i) R-MC 39 mAb strongly reactive with macrophages in the cortex and cortico-medullary zone (CMZ) and weakly with some scattered macrophages in the medulla, blood vessels and thymocytes; (ii) R-MC 40, 41 and 42 mAb specific for cortical macrophages and most CMZ macrophages; (iii) R-MC 43 and 44 mAb predominantly recognizing CMZ and medullary macrophages; (iv) R-MC 45 mAb strongly labelling granulocytes and weakly a subset of macrophages throughout the thymus and isolated cells in the medulla. The obtained results show considerable heterogeneity within mobile thymic non-lymphoid cells and the presence of specific or common antigens in macrophages of particular topographic localization in the rat thymus.
Colić
M
M
Institute for Experimental Medicine, Military Medical Academy, Belgrade, Yugoslavia.
Popović
L J
LJ
Gasić
S
S
Dragojević-Simić
V
V
Milićević
N M
NM
Matanović
D
D
Dujić
A
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006098:Granulocytes / Q000276:immunology*; D007124:Immunoenzyme Techniques; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051381:Rats; D013950:Thymus Gland / Q000276:immunology*
D000911:Antibodies, Monoclonal