The relationship between P elements and male recombination in Drosophila melanogaster.
P element dysgenesis associated male recombination in Drosophila was examined with a selective system focused upon 5% of the standard female genetic map divided into eight recombination segments. We found no correspondence between P element mobilization events and recombination in males in the intervals monitored. We defined two adjacent short genetic and molecular regions, one devoid of male recombination and the other acting as a "hot spot" for exchange in the absence of supporting P element insertion and excision activity. These data suggest that, even in the presence of mobilizing P elements, transposase may be active at non-P element sites, and that the genome may harbor sequences ranging from highly responsive to completely unresponsive to transposase action. A viewpoint is presented wherein P elements, with sequences that bind transposase, serve to focus the recombination action of transposase to encompass a region of DNA radiating outward from the initial binding site. We suggest that this region is measured in terms of chromosomal segments rather than limited to P element sequences.
Duttaroy
A
A
Department of Molecular and Cell Biology, University of Connecticut, Storrs 06268.
McCarron
M
M
Sitaraman
K
K
Doughty
G
G
Chovnick
A
A
D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D003434:Crossing Over, Genetic; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D015321:Gene Rearrangement; D008297:Male; D011995:Recombination, Genetic
D004251:DNA Transposable Elements