Analysis of binding of a technetium-99m-labeled monoclonal antibody to lentivirus-infected cells.
Caprine arthritis-encephalitis (CAE) is a model for the study of lentiviral infections. The authors' hypothesis is that radioimmunodetection has the potential to detect lentiviral proteins at the surface of infected cells. A monoclonal antibody (CAEV92A1) specific for a CAE virus (CAEV)-associated glycoprotein and a control antibody were radiolabeled with technetium-99m (99mTc) using the pretinning method. Cell binding assays were used to evaluate immunoreactivity and binding properties of 99mTc-labeled antibodies to CAEV-infected cells. 99mTc-CAEV92A1 bound preferentially to paraformaldehyde-fixed and live CAEV-infected cells. 99mTc-CAEV92A1 did not appear to be shed rapidly from its binding site.
Papageorges
M
M
Department of Veterinary Clinical Medicine and Surgery, Washington State University, Pullman 99164-6610.
Gavin
P R
PR
Adams
D S
DS
Cheevers
W P
WP
Barbee
D D
DD
Sande
R D
RD
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D016088:Arthritis-Encephalitis Virus, Caprine; D006023:Glycoproteins / Q000276:immunology*; D015511:Goat Diseases / Q000378:metabolism / Q000473:pathology*; D006041:Goats; D066298:In Vitro Techniques; D016180:Lentivirus Infections / Q000378:metabolism / Q000473:pathology*; D011485:Protein Binding; D013667:Technetium; D014764:Viral Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D014764:Viral Proteins; D013667:Technetium