title: Multicenter clinical evaluation of the Du Pont Herpchek HSV ELISA, a new rapid diagnostic test for the direct detection of herpes simplex virus.
abstract: A new 4h rapid enzyme-immunoassay for direct detection of herpes simplex virus (HSV) antigen (Du Pont Herpchek) was evaluated with 743 clinical samples collected at obstetrics and gynecology (OB/GYN), sexually transmitted diseases (STD), and ophthalmology clinics. The sensitivity and specificity of Herpchek was 98.0% and 98.4% respectively compared to virus isolation in cell culture. Confirmatory blocking ELISA tests, clinical history and follow up indicate that the true specificity of the test is 100%.
authors.affiliation: Department of Obstetrics and Gynecology, State University of New York, Stony Brook 11794.
authors.forename: D A
authors.initials: DA
authors.lastname: Baker
authors.forename: D
authors.initials: D
authors.lastname: Pavan-Langston
authors.forename: B
authors.initials: B
authors.lastname: Gonik
authors.forename: P O
authors.initials: PO
authors.lastname: Milch
authors.forename: E C
authors.initials: EC
authors.lastname: Dunkel
authors.forename: A
authors.initials: A
authors.lastname: Berkowitz
authors.forename: M B
authors.initials: MB
authors.lastname: Woodin
authors.forename: A
authors.initials: A
authors.lastname: Philip
authors.forename: R J
authors.initials: RJ
authors.lastname: Vander-Mallie
authors.forename: M N
authors.initials: MN
authors.lastname: Bobrow
mesh_terms: D000328:Adult; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D015828:Eye Infections, Viral / Q000175:diagnosis; D005260:Female; D006561:Herpes Simplex / Q000175:diagnosis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D012749:Sexually Transmitted Diseases / Q000175:diagnosis; D018139:Simplexvirus / Q000302:isolation & purification*
chemical_list: D000956:Antigens, Viral; D011933:Reagent Kits, Diagnostic