Structural predictions for the central domain of dystrophin.
The amino acid sequence of dystrophin indicates that the molecule has globular N- and C-terminal domains separated by a long central rod domain. The central rod contains multiple repeats, about 100 amino acids long and of variable length. These diverge sufficiently in sequence that, in previous studies, only 14 of the most similar repeats have been aligned and analysed in any detail. We show here that a heptad pattern of hydrophobic residues is preserved across all repeats. Using the heptad pattern together with a consensus sequence template, we identified and aligned 25 repeats in the dystrophin rod sequence. Each repeat consists of a constant-length core helix of 54 residues, coupled via a short linker to a weakly conserved variable-length helix, and then via a second linker to the next core. The variable-length helix appears truncated in repeats 10 and 13 and extended in repeats 4 and 20. The extension of repeat 20 is particularly interesting since it corresponds to a hotspot of dystrophy-inducing mutations. Detailed modelling suggests that the classical Speicher-Marchesi [(1984) Nature 311, 177-180] model for spectrin may not be appropriate to dystrophin without some modification. We propose that whilst the repeating structural motif in dystrophin is probably a bead of triple coiled coil, this bead is twice as massive as, and out of phase with, those proposed for spectrin. Our model raises the possibility that the rod domain of dystrophin may confer elasticity on the molecule. Deletions which truncate this region would then reduce the extensibility of the molecule without affecting actin crosslinking, consistent with their typically producing the relatively benign Becker phenotype of muscular dystrophy.
Cross
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RA
MRC Laboratory of Molecular Biology, Cambridge, England.
Stewart
M
M
Kendrick-Jones
J
J
D000185:Actinin / Q000032:analysis; D000595:Amino Acid Sequence; D016189:Dystrophin; D008961:Models, Structural; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000032:analysis*; D009154:Mutation; D011487:Protein Conformation; D013049:Spectrin / Q000032:analysis
D016189:Dystrophin; D009124:Muscle Proteins; D000185:Actinin; D013049:Spectrin