Interleukin-1 beta analogues with markedly reduced pyrogenic activity can stimulate secretion of adrenocorticotropic hormone in rats.
We examined the adrenocorticotropic hormone-releasing activities of several human interleukin-1 beta analogues that have markedly reduced pyrogenic activities in rats. Among the analogues tested, [Gly4]-, [Leu93]- and [1-148]-interleukin-1 beta increased the plasma adrenocorticotropic hormone level to almost that induced by authentic human interleukin-1 beta. Modifications of the N-terminus of the authentic molecule, i.e., [7-153]- and [Des-Ala1, Asp4]-interleukin-1 beta, significantly reduced the hormone-releasing activity. These data suggest that the adrenocorticotropic hormone-releasing activity of human interleukin-1 beta resides in the N-terminal structure of the authentic peptide and can be separated from its pyrogenic activity.
Naito
Y
Y
Department of Anesthesia, Kyoto University Faculty of Medicine, Japan.
Fukata
J
J
Masui
Y
Y
Hirai
Y
Y
Murakami
N
N
Tominaga
T
T
Nakai
Y
Y
Tamai
S
S
Mori
K
K
Imura
H
H
D000324:Adrenocorticotropic Hormone / Q000097:blood / Q000378:metabolism*; D000818:Animals; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D008297:Male; D011749:Pyrogens; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D007375:Interleukin-1; D011749:Pyrogens; D000324:Adrenocorticotropic Hormone