Quantitative analysis of gamma-aminobutyric acid (GABA) receptors of Purkinje cell layer from rat cerebellar slices.
1. Extracellular recordings were made from Purkinje cell layer of rat cerebellar slices. Cell activity was sensitive to both magnesium and manganese ions. 2. Glutamic and aspartic acids both excited cell activity while gamma-aminobutyric acid (GABA), muscimol, taurine, beta-alanine and delta-aminolaevulinic acid all inhibited activity. 3. The sensitivity to GABA varied with depth while no such effect was observed with muscimol. 4. Bicuculline methiodide, picrotoxin and pitrazepin blocked the action of muscimol with pA2 values of 5.92, 5.97 and 5.71 respectively. 5. The benzodiazepines flurazepam and RU 32007 both potentiated the GABA inhibition and this potentiation was blocked by Ro 15-1788, a benzodiazepine antagonist.
Hussain
S
S
Department of Neurophysiology, School of Biochemical and Physiological Sciences, University of Southampton, Swindon, Wiltshire, U.K.
Bagust
J
J
Gardner
C R
CR
Ward
R A
RA
Walker
R J
RJ
D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004347:Drug Interactions; D005260:Female; D005971:Glutamates / Q000378:metabolism; D066298:In Vitro Techniques; D009118:Muscimol / Q000037:antagonists & inhibitors / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D011689:Purkinje Cells / Q000378:metabolism*; D051381:Rats; D011963:Receptors, GABA-A / Q000187:drug effects*; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D005971:Glutamates; D011963:Receptors, GABA-A; D009118:Muscimol; D001224:Aspartic Acid; D005680:gamma-Aminobutyric Acid