Active oxygen radicals produced by leukocytes of malignant lymphoma.
The electron spin resonance (ESR) spectra of active oxygen radicals produced during the respiratory burst of PMA-stimulated leukocytes and oxygen consumption are studied by ESR spin trapping and spin probe oxymetry for 31 times in 17 cases of malignant lymphoma. The results showed that the spectra produced from PMA-stimulated patients' leukocytes were predominantly spin adducts of DMPO-hydroxyl radicals (DMPO-OH). The decrease in oxygen consumption during respiratory burst suggested that respiratory burst function was inhibited. Kinetic observations of patient condition showed that respiratory burst and oxygen consumption were improved and even approached normal, when the patient was in remission or the condition was ameliorated. This paper is the first of its type and helps clarify the mechanism of malignant proliferation and metastasis.
Li
X J
XJ
Institute of Biophysics, Academia Sinica, Beijing.
Zhao
B L
BL
Hou
J W
JW
Xin
W J
WJ
Xie
R F
RF
Chen
Y R
YR
D000328:Adult; D000368:Aged; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D003520:Cyclophosphamide / Q000008:administration & dosage; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D005609:Free Radicals; D006689:Hodgkin Disease / Q000188:drug therapy / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008223:Lymphoma / Q000188:drug therapy / Q000378:metabolism*; D008297:Male; D008466:Mechlorethamine / Q000008:administration & dosage; D008875:Middle Aged; D010101:Oxygen Consumption; D011241:Prednisone / Q000008:administration & dosage; D011344:Procarbazine / Q000008:administration & dosage; D013481:Superoxides / Q000378:metabolism; D014750:Vincristine / Q000008:administration & dosage
D005609:Free Radicals; D013481:Superoxides; D011344:Procarbazine; D008466:Mechlorethamine; D014750:Vincristine; D003520:Cyclophosphamide; D011241:Prednisone