Production of monoclonal antibody to bovine leukemia virus glycoprotein gp51 by in vitro immunization.
The monoclonal antibody against glycoprotein gp51 of bovine leukemia virus (BLV) envelope antigen was produced by in vitro immunization. This monoclonal antibody reacted with viral antigen was observed at the 69 kilodalton (kDa) glycoprotein. However, this monoclonal antibody was not involved in neutralizing. It was shown that in comparison with in vivo immunization, in vitro immunization has some advantages, namely a short immunization period and a small antigen quantity.
Sasaki
O
O
Department of Microbiology, Kyoto Prefectural University of Medicine.
Tanaka
M
M
Okabe
T
T
Imanishi
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D007114:Immunization; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D051379:Mice; D012190:Retroviridae / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D014759:Viral Envelope Proteins