Production of humoral factors that stimulate spleen colony-forming units in mice irradiated with moderate doses of X rays.
The production of humoral factors that stimulate spleen colony-forming units (CFU-S) has been studied in irradiated mice using an in vivo diffusion chamber assay. The experiments show that a significant release of factors that stimulate CFU-S takes place in the first few days after irradiation with moderate doses of 1.5 or 5 Gy. In contrast, the release of significant amounts of these humoral factors was not seen in animals irradiated with either low (0.75 Gy) or high (10 Gy) doses of X rays. The correlation observed between the production of factors that stimulate the CFU-S and the hemopoietic regeneration kinetics of the irradiated mice suggests that these factors represent part of the physiological regulators controlling the proliferation of CFU-S.
Grande
T
T
Unidad de Biología Molecular y Celular, Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT), Madrid, Spain.
González
J
J
Tejero
C
C
Maganto
G
G
Bueren
J A
JA
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000917:Antibody Formation; D002455:Cell Division / Q000528:radiation effects; D015194:Diffusion Chambers, Culture; D004731:Endotoxins / Q000097:blood; D005260:Female; D051379:Mice; D008815:Mice, Inbred Strains; D011829:Radiation Dosage; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000528:radiation effects*; D013234:Stem Cells / Q000166:cytology / Q000276:immunology / Q000528:radiation effects*; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014916:Whole-Body Irradiation
D000906:Antibodies; D004731:Endotoxins; D013936:Thymidine