Proteoglycans detected by monoclonal antibodies in adenoid cystic carcinoma of salivary glands.
Thirty-two cases of adenoid cystic carcinoma (ACC) of the salivary glands were examined immunohistochemically by monoclonal antibodies to proteoglycans (PG) such as chondroitin 6 sulfate (C6SPG), chondrotin 4 sulfate (C4SPG), dermatan sulfate (DSPG), heparan sulfate (HSPG) and keratan sulfate (KSPG) in conjunction with specific enzymatic digestion. The cribriform structure of ACC consisted of basaloid, myoepithelium-derived, and luminal tumor cells. The myoepithelial tumor cells were positive for PG, whereas luminal tumor cells were unstained. Occasional pseudocysts also gave positive staining for PG. Tubular structures consisting of modified myoepithelial cells indicated a high intensity reaction for C6SPG and C4SPG, and a slight one for DSPG, HSPG, and KSPG. Immunodepositions in solid and cluster structures were comparatively light in terms of PG. Basement membrane in ACC stained strongly for C4SPG, slightly for C6SPG, and very slightly for DSPG, HSPG, and KSPG. In ACC, immunohistochemical staining of PG was regularly positive in myoepithelium-derived tumor cells, but was irregular in other types of tumor cells.
Hashimura
K
K
Department of Oral and Maxillofacial Surgery, Asahi University School of Dentistry, Gifu, Japan.
Kasai
T
T
Yamada
K
K
Mori
M
M
Chomette
G
G
Auriol
M
M
Vaillant
J M
JM
D000911:Antibodies, Monoclonal; D003528:Carcinoma, Adenoid Cystic / Q000032:analysis* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D011509:Proteoglycans / Q000032:analysis* / Q000276:immunology; D012468:Salivary Gland Neoplasms / Q000032:analysis* / Q000473:pathology; D012469:Salivary Glands / Q000032:analysis
D000911:Antibodies, Monoclonal; D011509:Proteoglycans