The chicken c-erbA alpha-product induces expression of thyroid hormone-responsive genes in 3,5,3'-triiodothyronine receptor-deficient rat hepatoma cells.
To determine the capacity of the chicken c-erbA (cTR-alpha) gene product in regulating expression of known thyroid hormone-responsive genes, both the cTR-alpha and the viral v-erbA genes were expressed in FAO cells, a rat hepatoma cell line defective for functional thyroid hormone receptors. Upon nuclear expression of the cTR-alpha protein the cells become responsive to thyroid hormone, as detected by expression of a number of genes (malic enzyme, phosphoenolpyruvate carboxykinase, and Na+/K(+)-ATPase) reported to be indirectly induced by the hormone in vivo. In addition, our data show that the c-erbA product directly activates the Moloney murine leukemia virus promoter in a ligand-dependent manner. The data show that the chicken c-erbA-alpha protein can modulate the expression of rat genes under either direct or indirect control by thyroid hormone.
Muñoz
A
A
Differentiation Programme, European Molecular Biology Laboratory, Heidelberg, West Germany.
Höppner
W
W
Sap
J
J
Brady
G
G
Nordström
K
K
Seitz
H J
HJ
Vennström
B
B
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003513:Cycloheximide / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects*; D005796:Genes / Q000187:drug effects*; D015698:Genomic Library; D008114:Liver Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure*; D008291:Malate Dehydrogenase / Q000235:genetics; D008969:Molecular Sequence Data; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000235:genetics; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000378:metabolism / Q000494:pharmacology* / Q000502:physiology; D051381:Rats; D011988:Receptors, Thyroid Hormone / Q000172:deficiency / Q000378:metabolism*; D012192:Retroviridae Infections / Q000235:genetics; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics; D013963:Thyroid Hormones / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects; D014284:Triiodothyronine / Q000378:metabolism / Q000494:pharmacology
D011518:Proto-Oncogene Proteins; D011988:Receptors, Thyroid Hormone; D013963:Thyroid Hormones; D014284:Triiodothyronine; D003513:Cycloheximide; D008291:Malate Dehydrogenase; D010729:Phosphoenolpyruvate Carboxykinase (GTP); D000254:Sodium-Potassium-Exchanging ATPase