Calcitonin gene-related peptide (CGRP) receptors are linked to cyclic adenosine monophosphate production in SK-N-MC human neuroblastoma cells.
Calcitonin gene-related peptide (CGRP) stimulated cyclic adenosine monophosphate (cAMP) levels in SK-N-MC human neuroblastoma cells in a time- and concentration-dependent manner. The efficacy order for CGRPs was human alpha-CGRP = human beta-CGRP = chick CGRP greater than rat CGRP greater than human [Tyr0]CGRP. Calcitonin (CT) failed to influence cAMP production in SK-N-MC cells. [Tyr0]CGRP27-37 which by itself did not affect cAMP levels antagonized CGRP action. Saturation analysis using [125I]CGRP showed a homogeneous population of binding sites. CGRP but not CT, vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) inhibited radioligand binding. Our results provide evidence that human neuroblastoma SK-N-MC cells contain highly specific CGRP receptors which are positively coupled to cAMP generation.
Van Valen
F
F
Department of Pediatric Hematology and Oncology, Heinrich-Heine University Medical Center, Düsseldorf, F.R.G.
Piechot
G
G
Jürgens
H
H
D001665:Binding Sites; D001667:Binding, Competitive; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism* / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis*; D006801:Humans; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology; D009478:Neuropeptide Y / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism* / Q000473:pathology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D009478:Neuropeptide Y; D011956:Receptors, Cell Surface; D014660:Vasoactive Intestinal Peptide; D000242:Cyclic AMP; D015740:Calcitonin Gene-Related Peptide