title: Vasopressin gene expression is stimulated by cyclic AMP in homologous and heterologous expression systems.
abstract: The possible role of cyclic AMP (cAMP) in the regulation of the vasopressin (VP) gene was tested in two cellular expression systems: one cell line with endogenous VP expression and the other which was transiently with a VP promoter-luciferase fusion gene. 8,Bromo-cAMP stimulated the VP mRNA content about 4-fold in the human VP-expressing small cell lung carcinoma cell line GLC-8. The luciferase activity in P19 embryonal carcinoma cells which were transiently transfected with -174 to +44 of the 5'-flanking region of the human VP gene linked to the firefly luciferase gene, was stimulated about 2-fold by the cAMP analogue. The results indicate that cAMP plays a role in the upregulation of the VP gene and hence point to several putative nucleotide motives in the promoter functionally conferring this response.
authors.affiliation: Rudolf Magnus Institute, Medical Faculty, University of Utrecht, The Netherlands.
authors.forename: M A
authors.initials: MA
authors.lastname: Verbeeck
authors.forename: R A
authors.initials: RA
authors.lastname: Adan
authors.forename: J P
authors.initials: JP
authors.lastname: Burbach
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000199:Actins / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D018288:Carcinoma, Small Cell; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000494:pharmacology*; D004274:DNA, Recombinant; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008175:Lung Neoplasms; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D014162:Transfection; D014407:Tumor Cells, Cultured; D014667:Vasopressins / Q000235:genetics*
chemical_list: D000199:Actins; D004274:DNA, Recombinant; D012333:RNA, Messenger; D014667:Vasopressins; D015124:8-Bromo Cyclic Adenosine Monophosphate; D000242:Cyclic AMP; D008156:Luciferases