Na+, K(+)-ATPase in cat salivary glands and changes induced by nerve stimulation: an immunohistochemical study.
The enzyme Na+, K(+)-ATPase was localized immunohistochemically in major salivary glands of the cat before and after autonomic nerve stimulation. Immunostaining was limited to basolateral plasma membranes. Cells lining striated and excretory ducts contained abundant Na+, K(+)-ATPase and showed no changes with neural stimulation. Serous-type cells in resting glands varied in reactivity, showing weak to moderate staining intensity in the parotid gland and more uniform staining of greater intensity in the sublingual gland. In contrast, demilune cells in the resting submandibular gland showed little if any staining. Mucous-type cells were negative in all glands. Parasympathetic stimulation promoted a gradual increase in immunostaining of submandibular demilune cells, which became marked with time. Sympathetic stimulation produced no detectable changes in Na+, K(+)-ATPase immunoreactivity in any site. These results support the concept that basolateral Na+, K(+)-ATPase is essential to the formation of a near-isotonic primary saliva by serous-type cells. The mechanism whereby parasympathetic stimulation evokes a marked flow of submandibular saliva remains unexplained, but has now been shown to involve a marked increase in the immunoreactivity of Na+, K(+)-ATPase at the base of the gland's demilune cells.
Winston
D C
DC
Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston 29425.
Schulte
B A
BA
Garrett
J R
JR
Proctor
G B
GB
D000818:Animals; D001341:Autonomic Nervous System / Q000502:physiology*; D002415:Cats; D002462:Cell Membrane / Q000201:enzymology; D004558:Electric Stimulation; D005260:Female; D005404:Fixatives; D007150:Immunohistochemistry; D008297:Male; D010275:Parasympathetic Nervous System / Q000502:physiology; D010306:Parotid Gland / Q000201:enzymology; D012463:Saliva / Q000378:metabolism; D012469:Salivary Glands / Q000201:enzymology* / Q000294:innervation; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013361:Sublingual Gland / Q000201:enzymology; D013564:Sympathetic Nervous System
D005404:Fixatives; D000254:Sodium-Potassium-Exchanging ATPase