Dengue-2 virus infection of human mononuclear cell lines and establishment of persistent infections.
Twenty three human mononuclear cell lines including ten myelomonocytic cell lines, eight B cell lines and five T cell lines, were examined to determine whether they could be infected with dengue-2 virus. All the cell lines were infected with dengue-2 virus as determined by immunofluorescent staining and by virus titration of culture supernatant fluids. K562, Jiyoye and Jurkat, respectively, showed the highest percentage of infected cells of these myelomonocytic, B and T cell lines. Antibody to dengue-2 virus at subneutralizing concentrations augmented dengue-2 virus infection of myelomonocytic cell lines, but not of B cell lines or of T cell lines. Persistent dengue-2 virus infection was established using a myelomonocytic cell line (K562), a B cell line (Raji), and a T cell line (HSB-2). These cell lines maintained a high percentage (more than 70%) of dengue-2 virus antigen-positive cells for at least 25 weeks. Very low titers of infectious dengue-2 virus were detected in the culture supernatant fluids of the persistently infected cells. Dengue-2 virus antigen-positive Raji cell clones were established from persistently-infected Raji cells using limiting dilutions and all of the cells in these clones were dengue-2 virus antigen-positive. These findings demonstrate that a variety of human mononuclear cell lines can be infected with dengue-2 virus and may be useful as models for the analysis of dengue virus-human cell interactions in dengue virus infections.
Kurane
I
I
Department of Medicine, University of Massachusetts Medical Center, Worcester.
Kontny
U
U
Janus
J
J
Ennis
F A
FA
D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line / Q000276:immunology / Q000382:microbiology; D002478:Cells, Cultured / Q000276:immunology / Q000382:microbiology; D003715:Dengue / Q000276:immunology / Q000382:microbiology*; D003716:Dengue Virus / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity; D005455:Fluorescent Antibody Technique; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000382:microbiology*
D000956:Antigens, Viral