Role of DNA topoisomerase I in the replication of herpes simplex virus type 2.
Camptothecin (CPT), a specific inhibitor of DNA topoisomerase I, effectively inhibited the replication of herpes simplex virus type 2 (HSV-2). CPT was found to reduce the level of immediate early viral mRNA in a dose-response manner. On the other hand, we could not find any evidence that a novel DNA topoisomerase I was induced in HSV-2-infected cells. Our observations suggest that host cell DNA topoisomerase I is involved in the replication of HSV-2.
Yamada
Y
Y
Laboratory of Virology, Nagoya University School of Medicine, Japan.
Yamamoto
N
N
Maeno
K
K
Nishiyama
Y
Y
D002166:Camptothecin / Q000494:pharmacology; D004264:DNA Topoisomerases, Type I / Q000096:biosynthesis / Q000502:physiology*; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects / Q000502:physiology; D018139:Simplexvirus / Q000187:drug effects / Q000502:physiology*; D059004:Topoisomerase I Inhibitors; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000187:drug effects / Q000502:physiology*
D059004:Topoisomerase I Inhibitors; D004264:DNA Topoisomerases, Type I; D002166:Camptothecin