Inhibition of endoproteolytic cleavage of cytomegalovirus (HCMV) glycoprotein B by palmitoyl-peptidyl-chloromethyl ketone.
Endoproteolytic cleavage of glycoprotein B (gB) of human cytomegalovirus (HCMV) is inhibited by palmitoylated peptidyl-chloromethyl ketone (palFAKR-CEK) at concentrations above 30 microM. Inhibitor treatment of HCMV-infected human fibroblasts neither interfered with exposure of gB on the plasma membrane, detected by surface membrane immunostaining, nor reduced production of intracellular infectious viral progeny. Release of infectious virus, on the other hand, was impaired.
Brücher
K H
KH
Institut für Virologie, Philipps-Universität Marburg, Federal Republic of Germany.
Garten
W
W
Klenk
H D
HD
Shaw
E
E
Radsak
K
K
D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology*; D000595:Amino Acid Sequence; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D003587:Cytomegalovirus / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D010450:Endopeptidases / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000382:microbiology / Q000648:ultrastructure; D006801:Humans; D008969:Molecular Sequence Data; D010168:Palmitates / Q000494:pharmacology*; D014759:Viral Envelope Proteins / Q000378:metabolism*; D014779:Virus Replication
D000590:Amino Acid Chloromethyl Ketones; D010168:Palmitates; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; D010450:Endopeptidases