title: Cyclic nucleotide phosphodiesterases and methyltransferases in purified lymphocytes, monocytes and polymorphonuclear leucocytes from healthy donors and asthmatic patients.
abstract: Cyclic nucleotide phosphodiesterase and phospholipid N-methyl-transferase activities were simultaneously measured in purified polymorphonuclear cell-, mononuclear cell-, lymphocyte- and monocyte-homogenates from control subjects, from patients with atopic asthma and from patients with non-atopic asthma. Whereas cyclic AMP and cyclic GMP phosphodiesterase activities were found to be about 10-fold lower in polymorphonuclear than in mononuclear cells, phospholipid N-methyltransferase proved to be rather similar in each cell type from control donors. Cyclic AMP phosphodiesterase and phospholipid N-methyltransferase were significantly decreased in polymorphonuclear cells and monocytes from asthmatic patients compared with the control group while cyclic GMP phosphodiesterase was significantly impaired only in the monocyte subpopulation.
authors.affiliation: Unité 205 INSERM, Institut National des Sciences appliquées de Lyon, France.
authors.forename: A F
authors.initials: AF
authors.lastname: Prigent
authors.forename: P
authors.initials: P
authors.lastname: Fonlupt
authors.forename: M
authors.initials: M
authors.lastname: Dubois
authors.forename: G
authors.initials: G
authors.lastname: Némoz
authors.forename: L
authors.initials: L
authors.lastname: Timouyasse
authors.forename: H
authors.initials: H
authors.lastname: Pacheco
authors.forename: Y
authors.initials: Y
authors.lastname: Pacheco
authors.forename: N
authors.initials: N
authors.lastname: Biot
authors.forename: M
authors.initials: M
authors.lastname: Perrin-Fayolle
mesh_terms: D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000097:blood*; D000293:Adolescent; D000328:Adult; D001249:Asthma / Q000201:enzymology*; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000201:enzymology; D008214:Lymphocytes / Q000201:enzymology; D008297:Male; D008780:Methyltransferases / Q000097:blood*; D008875:Middle Aged; D009000:Monocytes / Q000201:enzymology; D009504:Neutrophils / Q000201:enzymology; D050918:Phosphatidylethanolamine N-Methyltransferase
chemical_list: D008780:Methyltransferases; D050918:Phosphatidylethanolamine N-Methyltransferase; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases