title: Nationwide surveillance program to identify diarrhea-causing Escherichia coli in children in Thailand.
abstract: Escherichia coli strains isolated from children with diarrhea were collected from 16 hospitals in different districts in Thailand during 1985 and 1986 and submitted to the National Reference Laboratory. Isolates were identified by serogrouping or as enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), enteropathogenic E. coli (EPEC) adhesin factor (EAF) E. coli, or Shiga-like-toxin (SLT)-producing E. coli by DNA hybridization. EPEC strains of known serogroups were isolated from 10%, ETEC strains were isolated from 6%, EAF E. coli strains were isolated from 4%, EIEC strains were isolated from less than 1%, and SLT-producing E. coli strains were isolated from none of 393 children with diarrhea. Among 278 children whose ages were recorded, the highest rate of isolation of EAF E. coli was 11% (9 of 85) from children less than 6 months old. ETEC was isolated from 5% (4 of 85) of children less than 6 months old, from 10% (12 of 118) of children 6 to 23 months old, and from 1% (1 of 75) of children greater than 23 months old. EPEC strains of known serogroups were isolated from 18% (15 of 85) of children less than 6 months old, from 11% (13 of 118) of children 6 to 23 months old, and from 9% (7 of 75) of children greater than 23 months old. E. coli strains that hybridized with the EIEC probe were isolated from three children who were 20, 36, and 48 months old. Examining E. coli for hybridization with DNA probes for virulence determinants is a practical way of conducting nationwide surveillance of diarrhea-causing E. coli. Since only 33% (13 of 39) of EPEC serogroups hybridized with the EAF probe and none hybridized with the SLT probes, identification of EPEC by serogroups analysis, followed by serotyping, should continue to be used in the identification of EPEC.
authors.affiliation: Department of Medical Sciences, Thai Department of Public Health, Nonthaburi, Thailand.
authors.forename: R
authors.initials: R
authors.lastname: Sunthadvanich
authors.forename: D
authors.initials: D
authors.lastname: Chiewsilp
authors.forename: J
authors.initials: J
authors.lastname: Seriwatana
authors.forename: R
authors.initials: R
authors.lastname: Sakazaki
authors.forename: P
authors.initials: P
authors.lastname: Echeverria
mesh_terms: D002675:Child, Preschool; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D003967:Diarrhea / Q000382:microbiology*; D003968:Diarrhea, Infantile / Q000382:microbiology; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D009693:Nucleic Acid Hybridization; D012703:Serotyping; D013785:Thailand
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial