Detection of human papillomavirus in reprocessed routine Papanicolaou smears by DNA hybridization.
DNA dot blot hybridization was used for the detection of human papillomavirus (HPV) types 6, 11, 16, and 18 in reprocessed routinely collected Papanicolaou smears. DNA was extracted from the smears with alkaline lysis and applied onto a nitrocellulose filter. The specificity and sensitivity of the dot blot hybridization on reprocessed Papanicolaou smears were confirmed by Southern blot analysis of selected samples, using CaSki, SiHa cell lines as positive controls and HF 32 as negative controls. From 42 normal smears and 44 abnormal smears with koilocytosis present, 9 (21%) and 43 (98%), respectively, were positive for HPV 6/11/16 or 18. These results show that reprocessed Papanicolaou smears in combination with DNA hybridization have potential application in retrospective studies on the prevalence and distribution of HPV infection.
Rakoczy
P
P
Department of Microbiology, University of Western Australia.
Hutchinson
L
L
Kulski
J K
JK
Sterrett
G F
GF
Pixley
E C
EC
D015139:Blotting, Southern; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D006801:Humans; D015151:Immunoblotting; D009693:Nucleic Acid Hybridization; D065006:Papanicolaou Test; D027383:Papillomaviridae / Q000302:isolation & purification*; D014626:Vaginal Smears / Q000379:methods*
D015344:DNA Probes, HPV; D004279:DNA, Viral