Species-specific expression of cytochrome c oxidase subunit III mRNA.
1. A cDNA probe encoding cytochrome c oxidase subunit III cloned from rat liver mitochondria was used to quantify mRNA levels in rat, mouse and rabbit tissues. This was compared to its phenotypic expression, using enzyme activity. 2. Enzyme activities were highest in mouse, intermediate in rat, and lowest in rabbit tissues. 3. Subunit III mRNA levels were easily quantified in rat, but could not be accurately measured in rabbit or mouse tissues despite high cytochrome c oxidase activities. 4. Significant subunit III sequence divergence must exist, among these species. Caution should be exercised in quantifying the expression of this mitochondrial gene.
Hood
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DA
Faculty of Biology, University of Konstanz, FRG.
D000818:Animals; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D003576:Electron Transport Complex IV / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008930:Mitochondria, Liver / Q000201:enzymology; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
D015342:DNA Probes; D012333:RNA, Messenger; D004247:DNA; D003576:Electron Transport Complex IV