title: Genetic analysis of chromosomal operons involved in degradation of aromatic hydrocarbons in Pseudomonas putida TMB.
abstract: The catabolic pathway for the degradation of aromatic hydrocarbons encoded by Pseudomonas putida TMB differs from the TOL plasmid-encoded pathway as far as regulation of the upper pathway is concerned. We found, by analyzing Tn5-induced mutants and by Southern blot hybridization with appropriate probes derived from the TOL plasmid pWW0, that the catabolic genes of strain TMB were located on the bacterial chromosome and not on the 84-kb plasmid harbored by this strain. The catabolic genes of TMB and pWW0 had sequence homology, as shown by Southern blot hybridization, but differed significantly in their restriction patterns. The analysis of the mutants suggests that a regulatory mechanism similar to that present in pWW0 coexists in TMB with a second mode of regulation which is epistatic on the former and that the chromosomal region carrying the catabolic genes is prone to rearrangements and deletions.
authors.affiliation: Dipartimento di Genetica e di Biologia dei Microrganismi, Università degli Studi di Milano, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Polissi
authors.forename: G
authors.initials: G
authors.lastname: Bestetti
authors.forename: G
authors.initials: G
authors.lastname: Bertoni
authors.forename: E
authors.initials: E
authors.lastname: Galli
authors.forename: G
authors.initials: G
authors.lastname: Dehò
mesh_terms: D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003227:Conjugation, Genetic; D015342:DNA Probes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D006838:Hydrocarbons / Q000378:metabolism*; D016254:Mutagenesis, Insertional; D009154:Mutation; D009876:Operon; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics* / Q000378:metabolism
chemical_list: D015342:DNA Probes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D006838:Hydrocarbons