In-vitro enhancement of leukotriene B4 receptor expression on human neutrophils by cefadroxil.
The influence of cefadroxil on LTB4-receptor expression of polymorphonuclear leucocytes (PMNs) was studied. Furthermore, the effect of cefadroxil on the leukotriene generation from PMNs and the lymphocyte, monocyte and basophil (LMB) containing cell fraction as well as on the synthesis of 12-hydroxyeicosatetraenoic acid (12-HETE) from human platelets was analysed. Antibiotic concentrations ranged from 50 to 5 micrograms/10(7) cells. Analysis of the generated leukotrienes was performed by high performance liquid chromatography (HPLC). Significant augmentation of the LTB4-receptor expression in human PMNs (range 190%-220%) was observed at concentrations of 50 and 25 micrograms/10(7) cells. The calcium-ionophore A23187 induced LTB4 generation from PMNs as well as 12-HETE synthesis from platelets was not significantly modulated in the presence of cefadroxil. Preincubation of the human LMB fraction led to slight suppression of the ionophore induced LTB4 generation up to 20%.
Knöller
J
J
Lehrstuhl für Medizinische Mikrobiologie und Immunologie, Ruhr Universität Bochum, FRG.
Brom
J
J
Schönfeld
W
W
König
W
W
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D002434:Cefadroxil / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D006098:Granulocytes / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis; D066298:In Vitro Techniques; D007975:Leukotriene B4; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D011971:Receptors, Immunologic / Q000096:biosynthesis*; D018102:Receptors, Leukotriene B4
D006893:Hydroxyeicosatetraenoic Acids; D011971:Receptors, Immunologic; D018102:Receptors, Leukotriene B4; D007975:Leukotriene B4; D002434:Cefadroxil; D000001:Calcimycin; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid