Generation of cell-mediated cytotoxicity against bovine-papilloma-virus-transformed primary mouse cell lines.
The immunogenicity and immunosensitivity of primary mouse cell lines transformed by bovine papilloma virus 1 (BPV1) DNA were studied in a syngeneic mouse model by determining cell-mediated cytotoxicity in the spleens of mice immunized with the transformed cells. One of the cell lines induced the generation of cell-line-specific Thy1.2-positive cytotoxic effector cells. However, most of the cell lines tested induced the generation of Thy1.2-positive effector cells, which in addition to BPV1-transformed cells were able to lyse a syngeneic cell line transformed by methylcholanthrene. The lysis of BPV1- and methylcholanthrene-transformed cell lines was mediated by recognition of the same antigenic determinants expressed on these cells, and all the BPV1-transformed cell lines were sensitive to lysis by these nonspecific effector cells of the lymphokine-activated killer (LAK) type.
Laatikainen
A
A
Department of Clinical Microbiology, University of Kuopio, Finland.
Karjalainen
H
H
Sarkkinen
H
H
Syrjänen
K
K
Mäntyjärvi
R
R
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D052688:Bovine papillomavirus 1 / Q000235:genetics* / Q000276:immunology; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D003602:Cytotoxicity, Immunologic; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D027383:Papillomaviridae / Q000235:genetics*
D000956:Antigens, Viral