Novel fluorogenic substrates for phosphodiesterase I.
The fluorescence of 9,10-dioxa-syn-3,4,6,7-tetramethylbimane (bimane) was found to be quenched in the presence of guanosine 5'-monophosphate. By using this phenomenon, the bimane system was used for the fluorophor of substrates for phosphodiesterase I. Bimanes were coupled to 5'-guanylic acid and the resulting compounds were shown to be portent fluorogenic substrates for the assay of phosphodiesterase I.
Sato
E
E
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Yoshikawa
M
M
Kanaoka
Y
Y
D001643:Bridged Bicyclo Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; D005456:Fluorescent Dyes; D006157:Guanosine Monophosphate / Q000737:chemistry*; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases / Q000032:analysis*; D013379:Substrate Specificity
D001643:Bridged Bicyclo Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; D005456:Fluorescent Dyes; C021149:bimanes; D006157:Guanosine Monophosphate; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I