Effect of gomisin A (TJN-101) on the arachidonic acid cascade in macrophages.
It has been reported that leukotrienes (LTs) may play a role in inflammatory liver diseases, and several inhibitors of LTs show an inhibitory effect on experimental liver injuries. In this study, the effect of Gomisin A (TJN-101), which is a lignan component of schisandra fruits, on the arachidonic acid cascade in macrophages was examined to explain the mechanisms of the inhibitory effect of TJN-101 on liver injuries. The production of leukotriene B4 was suppressed by treatment with TJN-101, while the activity of 5-lipoxygenase was not affected. The release of arachidonic acid from macrophages stimulated with fMet-Leu-Phe or the Ca++ ionophore A23187 was suppressed by treatment with TJN-101. The activity of phospholipase A2 was not affected by treatment with TJN-101. These results suggested that TJN-101 produces an inhibitory effect on the biosynthesis of LTs by preventing the release of arachidonic acid, and it was thought that the preventive effect on the arachidonic acid cascade may be partially associated with the inhibitory effect of TJN-101 on liver injuries.
Ohkura
Y
Y
Third Department of Internal Medicine, Osaka City University Medical School, Japan.
Mizoguchi
Y
Y
Morisawa
S
S
Takeda
S
S
Aburada
M
M
Hosoya
E
E
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control; D034242:Cyclooctanes; D004149:Dioxoles; D005122:Exudates and Transudates / Q000187:drug effects / Q000378:metabolism; D007975:Leukotriene B4 / Q000096:biosynthesis; D017705:Lignans; D008084:Lipoxygenase / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010741:Phospholipases A / Q000037:antagonists & inhibitors; D054467:Phospholipases A2; D011083:Polycyclic Compounds / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D011919:Rats, Inbred Strains
D001095:Arachidonic Acids; D034242:Cyclooctanes; D004149:Dioxoles; D017705:Lignans; D011083:Polycyclic Compounds; D007975:Leukotriene B4; C033585:schizandrol B; D008084:Lipoxygenase; D010741:Phospholipases A; D054467:Phospholipases A2