title: An enzyme-linked immunosorbent assay for detection of bovine leukaemia virus p24 antibody in cattle.
abstract: An ELISA for detecting antibody to the bovine leukaemia virus (BLV) core protein p24 is described. The test uses p24 antigen purified from concentrated cell culture supernate by lectin-affinity chromatography and gel filtration. The sensitivity and specificity of the p24-ELISA for diagnosing BLV infection relative to the gp51 agar gel immunodiffusion test, were 98.1 and 96.7%, respectively. In the event of widespread use of gp51 based vaccines, the p24-ELISA should differentiate effectively between naturally infected and vaccinated animals.
authors.affiliation: Queensland Department of Primary Industries, Animal Research Institute, Yeerongpilly, Australia.
authors.forename: J B
authors.initials: JB
authors.lastname: Molloy
authors.forename: P J
authors.initials: PJ
authors.lastname: Walker
authors.forename: F C
authors.initials: FC
authors.lastname: Baldock
authors.forename: B J
authors.initials: BJ
authors.lastname: Rodwell
authors.forename: J A
authors.initials: JA
authors.lastname: Cowley
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D007938:Leukemia / Q000175:diagnosis / Q000662:veterinary*; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D012190:Retroviridae / Q000276:immunology*; D015682:Retroviridae Proteins, Oncogenic / Q000276:immunology*; D014758:Viral Core Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D015682:Retroviridae Proteins, Oncogenic; D014758:Viral Core Proteins