Aromatase activity of human adipose tissue stromal cells: effects of thyroid hormones and progestogens.
In order to determine the direct effects of thyroid hormones and progestogens on extraglandular aromatization, human adipose stromal cells in monolayer culture were used as a model system for this study on the regulation of aromatase enzyme activity. It was found that 1-thyroxine at 2- and 4-fold normal concentrations (220 and 440 nM, respectively) and triiodothyronine at 4-fold normal concentration (7.4 nM) had no effect on basal, dibutyryl cyclic AMP ((Bu)2 cAMP)-induced, or dexamethasone-induced aromatization. Medroxyprogesterone acetate at a concentration of 25.9 nM, but not progesterone, 47.7 nM, stimulated basal aromatization slightly but not significantly. In contrast, both medroxyprogesterone acetate and progesterone potentiated the effect of (Bu)2 cAMP on aromatase activity (P less than 0.05 and P less than 0.01, respectively) but had no effect on dexamethasone-stimulated aromatase activity. We concluded that (i) the increased peripheral aromatization associated with hyperthyroidism is not due to the direct effect of thyroid hormones on aromatase activity, and (ii) neither progesterone nor medroxyprogesterone acetate inhibit aromatase activity of adipose tissue stromal cells, but may stimulate this activity under certain conditions.
Lueprasitsakul
P
P
Department of Obstetrics and Gynecology, University of Massachusetts Medical School, Worcester 01655.
Longcope
C
C
D000273:Adipose Tissue / Q000187:drug effects / Q000201:enzymology*; D001141:Aromatase / Q000032:analysis*; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008297:Male; D008525:Medroxyprogesterone / Q000031:analogs & derivatives / Q000494:pharmacology; D017258:Medroxyprogesterone Acetate; D011374:Progesterone / Q000494:pharmacology*; D013974:Thyroxine / Q000494:pharmacology*
D011374:Progesterone; D003994:Bucladesine; D017258:Medroxyprogesterone Acetate; D001141:Aromatase; D008525:Medroxyprogesterone; D013974:Thyroxine