title: Immunoblotting analysis of concanavalin A-isolated allergens of Candida albicans.
abstract: The carbohydrate-containing fraction of Candida albicans was isolated from the crude extract with ConA Sepharose affinity chromatography and studied by IgE-immunoblotting with individual and pooled sera from C. albicans-allergic subjects. In the ConA-bound fraction there was a diffuse IgE binding in the high molecular weight area which also gave a carbohydrate stain (PAS). A distinct band corresponding to a molecular weight of 70 kD bound specific IgE antibodies. This glycoprotein, presumably a mannoprotein, gave a weak carbohydrate staining and a strong protein staining. Further biochemical studies are needed to reveal the exact nature of the epitopes in the ConA-bound mannose-containing fraction of C. albicans.
authors.affiliation: Department of Medical Microbiology, University of Turku, Finland.
authors.forename: J
authors.initials: J
authors.lastname: Savolainen
authors.forename: M
authors.initials: M
authors.lastname: Viander
authors.forename: R
authors.initials: R
authors.lastname: Einarsson
authors.forename: A
authors.initials: A
authors.lastname: Koivikko
mesh_terms: D000485:Allergens / Q000276:immunology*; D002176:Candida albicans / Q000276:immunology* / Q000302:isolation & purification; D003208:Concanavalin A / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007073:Immunoglobulin E / Q000378:metabolism; D008351:Mannans / Q000032:analysis* / Q000276:immunology
chemical_list: D000485:Allergens; D008351:Mannans; D003208:Concanavalin A; D007073:Immunoglobulin E