A new procedure for the isomerization of vitamin D and its metabolites.
A new procedure for the isomerization of vitamin D and its metabolites is described. Vitamin D or its metabolites are dissolved in 100 microliters methanol and 10 M HC1 in 2-butanol is used as the reagent for isomerization. The isomerization reaction is carried out at 5 degrees C for 2-3 min which gives quantitative yields of isotachysterols down to 10 ng level without use of any carrier.
Agarwal
V K
VK
Connecticut Agricultural Experiment Station, New Haven 06504.
D002112:Calcifediol; D002762:Cholecalciferol; D002851:Chromatography, High Pressure Liquid; D004872:Ergocalciferols; D004875:Ergosterol / Q000031:analogs & derivatives; D007536:Isomerism; D000432:Methanol; D012997:Solvents; D013696:Temperature; D013997:Time Factors; D014807:Vitamin D / Q000378:metabolism
D004872:Ergocalciferols; D012997:Solvents; D014807:Vitamin D; D002762:Cholecalciferol; C002960:isotachysterol; D002112:Calcifediol; D000432:Methanol; D004875:Ergosterol