Mouse hepatitis virus ORF 2a is expressed in the cytosol of infected mouse fibroblasts.
A 266-bp fragment of cDNA from within gene B, ORF 2a, of MHV-A59 was used to construct a vector encoding a bacterial/viral fusion protein. Antiserum raised against this fusion protein specifically immunoprecipitates a 30K protein from infected 17Cl-1 mouse fibroblasts. The protein is localized primarily in the cytosol and not in the membranes. This is consistent with its predicted sequence and potential role as an RNA binding protein.
Zoltick
P W
PW
Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia 19104-6076.
Leibowitz
J L
JL
Oleszak
E L
EL
Weiss
S R
SR
D000818:Animals; D002213:Capsid / Q000032:analysis* / Q000276:immunology; D002460:Cell Line; D003600:Cytosol / Q000032:analysis; D005347:Fibroblasts / Q000032:analysis; D005455:Fluorescent Antibody Technique; D007106:Immune Sera / Q000276:immunology; D051379:Mice; D006517:Murine hepatitis virus / Q000235:genetics*; D014758:Viral Core Proteins / Q000032:analysis* / Q000276:immunology; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins / Q000032:analysis*
D007106:Immune Sera; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins