An adenovirus recombinant that expresses the human cytomegalovirus major envelope glycoprotein and induces neutralizing antibodies.
The gene of the human cytomegalovirus (HCMV) major envelope glycoprotein, gB, was cloned from the Towne strain and inserted into adenovirus type 5 downstream of the E3 promoter. The recombinant virus, Ad-gB, expressed antigenically related proteins of 58, 30, 25, and 23 kDa in A549 and MRC-5 cells; the 58-kDa protein had the same mobility as the native gB from HCMV-infected MRC-5 cells and virions. All four proteins were detected by a monospecific polyclonal antiserum and by a monoclonal antibody in immunoblot and immunofluorescence assays. Hamsters infected intranasally with live Ad-gB developed protein-specific and HCMV-neutralizing antibody. This study confirms the importance of gB in the generation of the neutralizing immune response to HCMV and demonstrates the potential of live adenoviruses as vaccine vectors.
Marshall
G S
GS
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
Ricciardi
R P
RP
Rando
R F
RF
Puck
J
J
Ge
R W
RW
Plotkin
S A
SA
Gönczöl
E
E
D000260:Adenoviruses, Human / Q000235:genetics* / Q000276:immunology; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D015139:Blotting, Southern; D003001:Cloning, Molecular; D006224:Cricetinae; D003587:Cytomegalovirus / Q000235:genetics* / Q000276:immunology; D005455:Fluorescent Antibody Technique; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D006801:Humans; D015151:Immunoblotting; D015345:Oligonucleotide Probes; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology
D000914:Antibodies, Viral; D015345:Oligonucleotide Probes; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus