The synthesis of 5-iodoindoxyl-3-phenylphosphonate and its use in analysis of phosphodiesterase I.
5-Iodoindoxyl-3-phenylphosphonate was prepared by using phenylphosphonic dichloride and 5-iodo-1-acetylindoxyl-3-ol. Its physical and chemical properties were studied. The results show that it can be used as an alternative substrate of snake venom phosphodiesterase I for assay of zymogram and in histochemistry.
Han
G Y
GY
Jinan Institute of Medicine, Peoples Republic of China.
D000818:Animals; D002854:Chromatography, Paper; D004591:Electrophoresis, Polyacrylamide Gel; D007211:Indoles / Q000138:chemical synthesis*; D007668:Kidney / Q000201:enzymology; D009943:Organophosphorus Compounds / Q000138:chemical synthesis*; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases / Q000032:analysis*; D011817:Rabbits; D013379:Substrate Specificity
D007211:Indoles; D009943:Organophosphorus Compounds; C064088:5-iodoindoxyl-3-phenylphosphonate; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I; C470790:phosphodiesterase I, snake venom