13C and 31P NMR studies of the pentose phosphate pathway in human erythrocytes.
31P NMR was used to monitor the concentrations of some of the intermediates of the non-oxidative pentose phosphate pathway (PPP) during the dissimilation of inosine and phosphate in dilute haemolysates. The temperature dependence of the ketone/hydrate ratio in dihydroxyacetone phosphate and the relative proportions of the isomers of sedoheptulose 1,7-bisphosphate were measured. The 31P NMR time courses were compared with the simulations obtained with a computer model of the modified F-type PPP.
Kuchel
P W
PW
Department of Biochemistry, University of Sydney, NSW, Australia.
Berthon
H A
HA
Bubb
W A
WA
McIntyre
L M
LM
Nygh
N K
NK
Thorburn
D R
DR
D019747:Aldose-Ketose Isomerases; D002238:Carbohydrate Epimerases / Q000378:metabolism; D002247:Carbon Isotopes; D003198:Computer Simulation; D004099:Dihydroxyacetone Phosphate / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D007288:Inosine / Q000378:metabolism*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008954:Models, Biological; D010427:Pentose Phosphate Pathway; D010759:Phosphorus Isotopes; D010770:Phosphotransferases / Q000378:metabolism; D013403:Sugar Phosphates / Q000378:metabolism
D002247:Carbon Isotopes; D010759:Phosphorus Isotopes; D013403:Sugar Phosphates; D004099:Dihydroxyacetone Phosphate; D007288:Inosine; C022079:sedoheptulose 1,7-bisphosphate; D010770:Phosphotransferases; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases; C022548:ribosephosphate isomerase; C022375:phosphopentomutase