Changes in the protein synthesis pattern during a nutritional shift-down transition in Saccharomyces cerevisiae.
In Saccharomyces cerevisiae cells (strain A364A) during a shift-down from glucose to raffinose, a rapid reduction in the rate of RNA accumulation was observed whereas the rate of protein accumulation was unaffected for at least 2 h. Following the transition the percentage of unbudded cells slightly increased and the cell volume distribution showed a newly formed subpopulation of smaller cells. To study the effects of the shift-down on the protein synthesis pattern, total [35S]-methionine pulse-labeled extracts were fractionated by high-resolution two-dimensional gel electrophoresis. The synthesis of two classes of proteins (I and II) was modulated during the transitory state of growth: one positively, the other negatively. Two polypeptides of 57 kDa showed the most dramatic increase in synthesis during the shift-down. Also a heat-shock protein (HSP 256) appeared to be positively correlated to the shift-down transition.
Rodriguez
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F
Dipartimento di Fisiologia e Biochimica Generali, Università di Milano, Italy.
Popolo
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Vai
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M
Lacanà
E
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Alberghina
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L
D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D007700:Kinetics; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D010449:Peptide Mapping; D012331:RNA, Fungal / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000254:growth & development* / Q000378:metabolism
D005656:Fungal Proteins; D012331:RNA, Fungal; D008715:Methionine