Differential amplification of the TGF-alpha gene in human gliomas.
The gene amplification and expression of transforming growth factor-alpha (TGF-alpha) and the epidermal growth factor receptor (EGF-R) in human gliomas was determined by Southern blot analysis and a receptor binding study. Amplification of the EGF-R gene was demonstrated in 3 of 11 tumors examined. All were glioblastomas, whereas the TGF-alpha gene was amplified in 7 of 11 tumors, and 6 of the 7 were recurrent glioblastomas and anaplastic astrocytomas. A competitive binding study with iodinated EGF showed a TGF-alpha-like activity ranging from 1.6-31.5 ng of EGF/microgram protein. These results suggest a differential expression of EGF-R and TGF-alpha genes among untreated and recurrent malignant gliomas.
Yung
W K
WK
Department of Neuro-Oncology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Zhang
X
X
Steck
P A
PA
Hung
M C
MC
D001667:Binding, Competitive; D015139:Blotting, Southern; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D004815:Epidermal Growth Factor / Q000378:metabolism; D066246:ErbB Receptors / Q000235:genetics; D005784:Gene Amplification; D005909:Glioblastoma / Q000235:genetics / Q000473:pathology; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007457:Iodine Radioisotopes; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D007457:Iodine Radioisotopes; D014409:Tumor Necrosis Factor-alpha; D004815:Epidermal Growth Factor; D066246:ErbB Receptors