title: Construction of expression plasmids for the fusion protein of Sendai virus, and their expression in E. coli cells and eucaryotic cells.
abstract: To examine the properties and the role of the fusion protein (F) of Sendai virus at the molecular level, a plasmid, pUC-F, was constructed by inserting cDNA for the F protein into a pUC vector. Upon induction of E. coli cells transformed with pUC-F, a new protein was obtained, which was identified as Fo on Western blot analysis. The cDNA fragment for the F gene was excised from pUC-F and inserted into an eucaryotic expression vector, pSVL, to yield pSVL-F. COS-1 cells transfected with pSVL-F gave a band on SDS-gel electrophoresis which corresponded to the size of the Fo proteins.
authors.affiliation: Department of Physiological Chemistry, Tokyo Metropolitan Institute of Medical Science, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Taira
authors.forename: L
authors.initials: L
authors.lastname: Ranin
authors.forename: K
authors.initials: K
authors.lastname: Iwasaki
mesh_terms: D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D010222:Parainfluenza Virus 1, Human / Q000235:genetics*; D010957:Plasmids; D014170:Transformation, Genetic; D014760:Viral Fusion Proteins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D014760:Viral Fusion Proteins