RNA levels of neuronal nicotinic acetylcholine receptor subunits are differentially regulated in axotomized facial motoneurons: an in situ hybridization study.
In situ hybridization histochemistry using complementary RNA probes revealed that alpha 3 and beta 2 neuronal nicotinic acetylcholine receptor subunit mRNAs were expressed in 12% and 40% of facial motoneurons of the rat, respectively. The alpha 3 subunit mRNA signals disappeared in response to axotomy, whereas the beta 2 subunit mRNA signal was remarkably enhanced, suggesting that mRNA levels of receptor subunits are differentially regulated in axotomized motoneurons.
Senba
E
E
Howard Hughes Medical Institute, Salk Institute for Biological Studies, La Jolla, CA 92037.
Simmons
D M
DM
Wada
E
E
Wada
K
K
Swanson
L W
LW
D000818:Animals; D001369:Axons / Q000502:physiology*; D005154:Facial Nerve / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology; D008297:Male; D009046:Motor Neurons / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D011149:Pons / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011978:Receptors, Nicotinic / Q000378:metabolism*
D012333:RNA, Messenger; D011978:Receptors, Nicotinic