Trypanosoma cruzi: identification of a surface antigen restricted to the flagellar region of the infective form of the parasite.
A hybridoma cell line was derived from spleen cells of B6D2 mice infected with the Peru strain of Trypanosoma cruzi. The monoclonal antibody produced by this hybridoma, designated mAb20H1, reacts exclusively with molecular components of trypomastigotes, the infective form of the parasite. The results of indirect immunofluorescence and of immunoelectron microscopy with gold-tagged antibodies indicate that the 20H1 antigen is restricted to the surface of the part of the flagellum in contact with the cell body and to the surface of the cell body in the immediate vicinity of this organelle. Western blot analysis showed that the 20H1 antigen consists of four to five different molecules with sizes between 34 and 41 kDa, and that these molecules are glycoproteins with affinity for concanavalin A. In other strains of T. cruzi, mAb20H1 reacts with glycoproteins with apparent sizes that range between 37 and 43 kDa in the CL, Esmeraldo and Y strains, and between 41 and 45 kDa in the Silvio strain.
Saborio
J L
JL
Department of Molecular Biology and Biochemistry, University of California, Irvine 92717.
Wrightsman
R A
RA
Kazuko
S G
SG
Granger
B S
BS
Manning
J E
JE
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000953:Antigens, Protozoan / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis; D015153:Blotting, Western; D005407:Flagella / Q000276:immunology* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006825:Hybridomas; D007150:Immunohistochemistry; D051379:Mice; D008854:Microscopy, Electron; D014349:Trypanosoma cruzi / Q000276:immunology* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000954:Antigens, Surface