Characterization of a new type of variant of rat basophilic leukemia 2H3 cells presenting a different pattern of calcium signal.
We selected a new type of variant (designated 3C7) derived spontaneously from parental RBL-2H3 cells. 3C7 cells showed lower contact inhibition, anchorage dependency, and serotonin release activity than those of RBL-2H3 cells. We conclude that 3C7 cells are a transformant of RBL-2H3 cell with greater malignancy. The production of inositol bisphosphate and the release of Ca2+ from intracellular stores induced by IgE-antigen stimulation were enhanced in 3C7. Oscillation of [Ca2+]i in individual 3C7 cells was observed by a digital imaging microscopic technique. We propose that 3C7 cells are a useful model system for studies on the mechanisms of stimulus-secretion coupling and the relationships between malignant alterations and disorders of signal transduction.
Kato
K
K
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Nakanishi
M
M
Arata
Y
Y
Teshima
R
R
Terao
T
T
Miyamoto
H
H
Hanaoka
F
F
Ui
M
M
Masuzawa
Y
Y
Hiraishi
S
S
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002455:Cell Division; D004140:Dinitrophenols / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology; D007295:Inositol Phosphates / Q000378:metabolism; D015471:Leukemia, Basophilic, Acute / Q000378:metabolism* / Q000473:pathology; D010716:Phosphatidylinositols / Q000378:metabolism; D051381:Rats; D012701:Serotonin / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000276:immunology; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
D004140:Dinitrophenols; D007295:Inositol Phosphates; D010716:Phosphatidylinositols; C034638:dinitrophenyl-bovine serum albumin; D012710:Serum Albumin, Bovine; D012701:Serotonin; D007073:Immunoglobulin E; D002118:Calcium