Mastoparan increases membrane bound phosphatidylinositol kinase and phosphatidylinositol 4-monophosphate kinase activities in Madin-Darby canine kidney cells.
Synthetic wasp venom Mastoparan induced an increase of [3H] inositol phosphates levels and a corresponding decrease of [3H]inositol phospholipids levels in Madin-Darby canine kidney (MDCK) cells. The effect was dose (5-100 micrograms/ml) and time (1 to 15 min) dependent. Mastoparan also enhanced the endogenous activity of phosphatidylinositol kinase and phosphatidylinositol 4-monophosphate kinase. The effect was dose (25-75 micrograms/ml) and time dependent (1 to 15 min).
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Department of Physiology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814-4799.
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D019870:1-Phosphatidylinositol 4-Kinase; D000818:Animals; D001514:Bee Venoms / Q000494:pharmacology*; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D004285:Dogs; D036341:Intercellular Signaling Peptides and Proteins; D007668:Kidney / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D010455:Peptides; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols / Q000378:metabolism*; D010766:Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism*; D014862:Wasp Venoms / Q000494:pharmacology*
D001514:Bee Venoms; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; D014862:Wasp Venoms; C037178:phosphatidylinositol 4-phosphate; C037500:mastoparan; D010770:Phosphotransferases; D019870:1-Phosphatidylinositol 4-Kinase