Equine herpesvirus type 1: detection of viral DNA sequences in aborted fetuses with the polymerase chain reaction.
Primers and probes were selected from the gene encoding glycoprotein 13 (gp 13) of equine herpesvirus 1 (EHV-1). The polymerase chain reaction (PCR) was run on infected and noninfected cultured cells and on 63 specimens from 29 aborted equine fetuses. The results were evaluated by electrophoresis and dot-blot hybridization using an oligonucleotide probe labeled with biotin. In the infected samples electrophoresis showed a PCR product of about 280 base pairs. The dot-blot hybridization confirmed that this product contained EHV-1 DNA sequences. PCR took 4 h and hybridization another 14 h; the results were thus achieved within 24 h and were highly specific for EHV-1. Close concordance was found between the results of PCR and virus isolation.
Ballagi-Pordány
A
A
Department of Virology, National Veterinary Institute, Uppsala, Sweden.
Klingeborn
B
B
Flensburg
J
J
Belák
S
S
D000034:Abortion, Veterinary / Q000175:diagnosis*; D000818:Animals; D004279:DNA, Viral / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D005260:Female; D005333:Fetus / Q000382:microbiology; D006564:Herpesviridae / Q000235:genetics*; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D004861:Herpesvirus 1, Equid / Q000235:genetics* / Q000302:isolation & purification; D006734:Horse Diseases / Q000175:diagnosis*; D006736:Horses; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D010920:Placenta / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011247:Pregnancy
D004279:DNA, Viral; D015345:Oligonucleotide Probes