title: [T lymphocyte populations of the intestinal mucosa in celiac disease in children. Immunohistochemical study].
abstract: In order to study the distribution of lymphocyte subpopulations in a pathologic intestinal mucosa, the authors, instead of using the classic method by counting the number of lymphocytes, present an original method permitting the exploitation of quantified data from labelled surface cells by texture analyser coupled with a computerized system. We investigated 25 children presenting with chronic diarrhea and villous atrophy and 5 control subjects. Fifteen of the 25 children had celiac disease (10 active with total villous atrophy and 5, celiac disease in remission with healing mucosa), 5 cow's milk protein intolerance with total or partial villous atrophy and 5, chronic diarrhea with partial villous atrophy. Immunohistochemical study with monoclonal antibodies was carried out on frozen sections using a three-step immunoperoxidase technique. Compared with the 5 controls, patients with food intolerance (celiac disease and cow's milk protein intolerance) showed a significant increase of T suppressor lymphocytes (p less than 0.01 and p less than 0.05) in the epithelium, whereas there were more T helper lymphocytes in the lamina propria (p less than 0.05 and p less than 0.01). Non-treated celiac disease was distinguished from treated celiac disease by a marked increase in intra-epithelial T cytotoxic-suppressors. These results suggest that T cytotoxic-suppressors may be the mediators of the lesions observed in celiac disease.
authors.affiliation: Service de Médecine Infantile D, Gastroentérologie et Nutrition, CHU Purpan, Toulouse.
authors.forename: J P
authors.initials: JP
authors.lastname: Olives
authors.forename: J J
authors.initials: JJ
authors.lastname: Voigt
authors.forename: T
authors.initials: T
authors.lastname: al Saati
authors.forename: L
authors.initials: L
authors.lastname: Nonnenmacher
authors.forename: P
authors.initials: P
authors.lastname: Brousset
authors.forename: G
authors.initials: G
authors.lastname: Delsol
authors.forename: J
authors.initials: J
authors.lastname: Ghisolfi
mesh_terms: D000293:Adolescent; D000328:Adult; D002446:Celiac Disease / Q000276:immunology* / Q000473:pathology; D002465:Cell Movement; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D003967:Diarrhea / Q000276:immunology; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007413:Intestinal Mucosa / Q000276:immunology* / Q000473:pathology; D007787:Lactose Intolerance / Q000276:immunology; D007958:Leukocyte Count; D008297:Male; D008894:Milk Proteins / Q000009:adverse effects; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology
chemical_list: D008894:Milk Proteins
vernacular_title: Populations lymphocytaires T de la muqueuse intestinale au cours de la maladie coeliaque chez l'enfant. Etude immunohistochimique.