Inhibitors of the ATP/ADP antiporter suppress stimulation of mitochondrial respiration and H+ permeability by palmitate and anionic detergents.
The action of ATP/ADP-antiporter inhibitors upon the uncoupling effect of palmitate, detergents and 'classical' uncouplers has been studied. The uncoupling effect was estimated by stimulation of succinate oxidation and of H+ permeability of rat liver mitochondria in the presence of oligomycin. It is shown that carboxyatractylate (CAtr) and pyridoxal 5-phosphate (PLP) suppress the uncoupling induced by palmitate and the anionic detergents SDS and cholate, but do not affect that induced by the cationic detergents CTAB, by the non-ionic detergent Triton X-100, as well as by the 'classical' uncouplers FCCP and DNP. The results are discussed in terms of a concept assuming that the ATP/ADP-antiporter facilitates the electrophoretic export of hydrophobic anions from mitochondria.
Brustovetsky
N N
NN
Institute of Biological Physics, USSR Academy of Science, Pushchino, Moscow Region.
Dedukhova
V I
VI
Egorova
M V
MV
Mokhova
E N
EN
Skulachev
V P
VP
D000818:Animals; D000838:Anions; D001278:Atractyloside / Q000031:analogs & derivatives / Q000494:pharmacology; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D019826:Cholic Acid; D002793:Cholic Acids / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D000226:Mitochondrial ADP, ATP Translocases / Q000037:antagonists & inhibitors*; D009840:Oligomycins / Q000494:pharmacology; D010084:Oxidation-Reduction; D010101:Oxygen Consumption / Q000187:drug effects; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000494:pharmacology*; D010539:Permeability; D011522:Protons; D011732:Pyridoxal Phosphate / Q000494:pharmacology; D051381:Rats; D012402:Rotenone / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid; D014475:Uncoupling Agents / Q000494:pharmacology
D000838:Anions; D002793:Cholic Acids; D003902:Detergents; D009840:Oligomycins; D010169:Palmitic Acids; D011522:Protons; D013386:Succinates; D014475:Uncoupling Agents; D012402:Rotenone; D001278:Atractyloside; D019308:Palmitic Acid; D012967:Sodium Dodecyl Sulfate; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D011732:Pyridoxal Phosphate; D000226:Mitochondrial ADP, ATP Translocases; D019802:Succinic Acid; D019826:Cholic Acid; C003853:carboxyatractyloside