Distribution of fodrin in the keratinocyte in vivo and in vitro.
Distribution of fodrin in the keratinocyte, both in vivo and in vitro, was examined by immunofluorescence microscopy. In the rat epidermis in vivo, fodrin was localized in the cell periphery of the spinous layer of all the skins studied. In only the basal layer of the thick skin, however, fodrin was seen intensely in the cytoplasm. As in vitro keratinocytes, a mouse cell line (Pam 212) cultured in low (0.06 mM) as well as standard (1.87 mM) Ca2+ was examined. In low Ca2+, fodrin was observed throughout the cytoplasm without marked accumulation irrespective of the cell density. The cytoplasmic labeling in low Ca2+ looked filamentous and became aggregated when cells were treated with cytochalasin B; at least some of the aggregates coexisted with those of F-actin. In contrast, fodrin distribution was not affected with colchicine. On the other hand, in standard Ca2+, the protein became concentrated along the cell periphery and less conspicuous in the cytoplasm as the cells reached confluency. When cells were transferred from low to standard Ca2+, the distribution of fodrin changed accordingly within 180 min. The present results indicate that fodrin in the keratinocyte is likely to be associated with actin filaments and that it takes two different ways of distribution both in vivo and in vitro. The peripheral and the cytoplasmic labeling of in vivo and in vitro cells are likely to correspond. It may be that fodrin changes its localization according to the cell's proliferative activity.
Yoneda
K
K
Department of Dermatology, Faculty of Medicine, Kyoto University, Japan.
Fujimoto
T
T
Imamura
S
S
Ogawa
K
K
D000818:Animals; D002352:Carrier Proteins / Q000032:analysis*; D002478:Cells, Cultured; D003593:Cytoplasm / Q000032:analysis; D004817:Epidermis / Q000032:analysis; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D015603:Keratinocytes / Q000032:analysis* / Q000648:ultrastructure; D008840:Microfilament Proteins / Q000032:analysis*; D008856:Microscopy, Fluorescence; D051381:Rats; D013049:Spectrin
D002352:Carrier Proteins; D008840:Microfilament Proteins; D013049:Spectrin