Phagocytic response modifying reactivity of enzymatic cell wall digests of Nocardia opaca.
Aqueous extracts (ENOCW) and enzymatic digests of purified Nocardia opaca cell wall fragments, virtually free of muramyl peptides, were monitored for their phagocytic response modifying reactivity on polymorphonuclear leucocytes, separated or unseparated in whole human blood. In the presence of ENOCW a 74% increased production of superoxide during the respiratory burst of TPA-activated polymorphonuclear leukocytes was observed, as compared to the unprimed control. Delipidation of this preparation resulted in a further increase in reactivity (144%). Even in the presence of whole human blood, as a model for competitive binding in biological fluids, an enhanced generation of superoxide by TPA activated blood phagocytes remained detectable. A 37-75% decreased phagocytic reactivity in samples of HIV-seropositive blood was considerably restored in the presence of ENOCW.
Miesel
R
R
Anorganische Biochemie, Physiologisch-Chemisches Institut, Universität Tübingen, F.R.G.
Körner
S
S
Haas
R
R
Spinnler
C
C
Ehrenfeld
U
U
Weser
U
U
D000166:Acridines; D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology; D002473:Cell Wall / Q000276:immunology; D002918:Chymotrypsin; D005959:Glucosidases; D006679:HIV Seropositivity / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D008213:Lymphocyte Activation; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D009615:Nocardia / Q000276:immunology*; D010587:Phagocytosis; D011402:Pronase / Q000494:pharmacology; D012995:Solubility; D013481:Superoxides / Q000378:metabolism; D013552:Swine; D013755:Tetradecanoylphorbol Acetate
D000166:Acridines; D001426:Bacterial Proteins; D013481:Superoxides; C033472:10,10'-dimethyl-9,9'-biacridinium; D005959:Glucosidases; D002918:Chymotrypsin; D011402:Pronase; D013755:Tetradecanoylphorbol Acetate