title: IAPP/amylin gene transcriptional control region: evidence for negative regulation.
abstract: Aberrant expression of the islet amyloid polypeptide (IAPP) gene might be involved in the pathogenesis of non-insulin-dependent diabetes mellitus (NIDDM). Here, we report that IAPP promoter-luciferase constructs revealed tissue-specific activity. This activity was not mediated by cAMP. Sequential 5' deletions of the IAPP promoter caused a progressive derepression of the IAPP gene promoter in IAPP-producing cells. Comparison of the nucleotide sequence of the IAPP promoter with that of the insulin promoter (both active in pancreatic beta-cells) reveals two sequence elements of putative importance: an insulin enhancer-like sequence and an element which corresponds to a protected domain in rat insulin I gene promoter footprint experiments.
authors.affiliation: Institute of Molecular Biology and Medical Biotechnology, Utrecht, The Netherlands.
authors.forename: S
authors.initials: S
authors.lastname: Mosselman
authors.forename: J W
authors.initials: JW
authors.lastname: Höppener
authors.forename: L
authors.initials: L
authors.lastname: de Wit
authors.forename: W
authors.initials: W
authors.lastname: Soeller
authors.forename: C J
authors.initials: CJ
authors.lastname: Lips
authors.forename: H S
authors.initials: HS
authors.lastname: Jansz
mesh_terms: D000682:Amyloid / Q000235:genetics*; D001483:Base Sequence; D003994:Bucladesine / Q000494:pharmacology; D002872:Chromosome Deletion; D005576:Colforsin / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D007328:Insulin / Q000235:genetics; D058228:Islet Amyloid Polypeptide; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D008156:Luciferases / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000187:drug effects; D016385:TATA Box; D014407:Tumor Cells, Cultured
chemical_list: D000682:Amyloid; D007328:Insulin; D058228:Islet Amyloid Polypeptide; D005576:Colforsin; D003994:Bucladesine; D008156:Luciferases