title: Madin-Darby canine kidney cells. III. Aldosterone stimulates an apical H+/K+ pump.
abstract: Functionally and morphologically, Madin-Darby canine kidney (MDCK) cells resemble intercalated cells of urinary epithelia. Experiments were performed on domes of confluent MDCK monolayers to test for apical H+ secretion. Apical application of 10(-3) mol/l amiloride or of Na(+)-free solution significantly reduced the limiting pH gradient across the dome epithelium (delta pHd) consistent with inhibition of apical Na+/H+ exchange. Short-circuit current (SCC) measurements disclosed an acetazolamide-sensitive, (basolateral to apical) positive transepithelial current stimulated by 10(-7) mol/l aldosterone and inhibited by acidification of apical medium to pH = 4.5. Histochemical evaluation of carbonic anhydrase (CA) activity revealed cytoplasmic and apical-membrane-bound CA particularly in dome-forming cells. Apical substitution of Na+ by K+ increased delta pHd, whereas a reduction of K+ concentration to 0.5 mmol/l or addition of barium or omeprazole (10(-5) mol/l) to the apical superfusate reduced delta pHd by at least 75%. Aldosterone-stimulated SCC was completely abolished by the apical application of barium. We conclude that besides Na+/H+ exchange MDCK cells can express an apically located H(+)-K+ pump stimulated by aldosterone and inhibited directly by the anti-ulcer agent omeprazole or indirectly, either by blocking apical K+ recycling or by interfering with the CA-dependent intracellular formation of H+ ions.
authors.affiliation: Department of Physiology, University of Würzburg, Federal Republic of Germany.
authors.forename: H
authors.initials: H
authors.lastname: Oberleithner
authors.forename: W
authors.initials: W
authors.lastname: Steigner
authors.forename: S
authors.initials: S
authors.lastname: Silbernagl
authors.forename: U
authors.initials: U
authors.lastname: Vogel
authors.forename: G
authors.initials: G
authors.lastname: Gstraunthaler
authors.forename: W
authors.initials: W
authors.lastname: Pfaller
mesh_terms: D000450:Aldosterone / Q000494:pharmacology*; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001464:Barium / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D002256:Carbonic Anhydrases / Q000378:metabolism; D002460:Cell Line; D004285:Dogs; D004553:Electric Conductivity; D004848:Epithelium / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D009853:Omeprazole / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism*; D011522:Protons; D012964:Sodium / Q000494:pharmacology
chemical_list: D015221:Potassium Channels; D011522:Protons; D001464:Barium; D000450:Aldosterone; D000584:Amiloride; D012964:Sodium; D002256:Carbonic Anhydrases; D009853:Omeprazole; D011188:Potassium