title: A monoclonal antibody that neutralizes Epstein-Barr virus DNA polymerase activity.
abstract: An Epstein-Barr virus (EBV) specific monoclonal antibody (MAb), designated 55H3, was produced after immunizing BALB/c mice with 12-O-tetradecanoylphorbol-13-acetate (TPA) activated B95-8 cells. It was possible to demonstrate that this MAb detected an EBV-specific antigen(s) in the EBV genome-positive producer cell lines B95-8 and M-ABA by immunofluorescence. Immunoglobulin prepared from ascites fluid neutralized the activity of the EBV-encoded DNA polymerase, but not the alkaline DNase. By Western blotting, the 55H3 MAb reacts with an 85/80-KD polypeptide. The 55H3 should be useful in examining the role of EBV DNA polymerase in viral replication.
authors.affiliation: Department of Medical Microbiology and Immunology, Ohio State University Medical Center, Columbus 43210-1239.
authors.forename: C H
authors.initials: CH
authors.lastname: Tsai
authors.forename: M V
authors.initials: MV
authors.lastname: Williams
authors.forename: R
authors.initials: R
authors.lastname: Glaser
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology; D015153:Blotting, Western; D002460:Cell Line; D004259:DNA-Directed DNA Polymerase / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human / Q000201:enzymology / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D009500:Neutralization Tests; D019384:Nucleic Acid Synthesis Inhibitors
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D019384:Nucleic Acid Synthesis Inhibitors; D004259:DNA-Directed DNA Polymerase