[The optimization of immunoenzyme analysis with conjugates of virus-specific antibodies and alkaline phosphatase].
The data obtained in the study of the dependence of sensitivity of enzyme immunoassay (EIA) on chromo- and fluorogenic substrates used in this assay are presented. The sandwich variant of EIA, carried out with the use of antibodies labeled with alkaline phosphatase, has been shown to be 4-170 times, sometimes 500 times, more sensitive (in terms of concentrations at which aphthous fever virus antigens can be detected) than the complement fixation test and 1.8-64 times more sensitive than the passive hemagglutination test.
Mishchenko
V A
VA
Bazarov
M A
MA
Smirnov
A B
AB
Koniushkina
T B
TB
D000469:Alkaline Phosphatase; D000914:Antibodies, Viral; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000032:analysis; D005537:Aphthovirus / Q000276:immunology*; D003168:Complement Fixation Tests; D005069:Evaluation Studies as Topic; D006386:Hemagglutination Tests; D007124:Immunoenzyme Techniques
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000469:Alkaline Phosphatase
Optimizatsiia immunofermentnogo analiza s kon''iugatami virus-spetsificheskikh antitel i shchelochnoĭ fosfatazy.