Identification and expression of the Escherichia coli fdhD and fdhE genes, which are involved in the formation of respiratory formate dehydrogenase.
The two closely linked fdhD and fdhE genes of Escherichia coli are required for the formation of active membrane-bound phenazine methosulfate-linked formate dehydrogenase (FDH-PMS). Both genes were isolated from a cosmid library. Restriction endonuclease analysis associated with Mu dII1734 insertion mutagenesis indicated that the two genes were separated by at least 4 kilobases and transcribed in opposite orientations. Initial experiments indicate that the region between the two genes seems not to be essential to FDH-PMS activity. fdhD and fdhE were expressed either in maxicells or from the T7 promoter-polymerase system. They were shown to encode proteins with approximate Mr 30,500 and 32,000, respectively. Both proteins appeared in the soluble fraction and were not recognized by an FDH-PMS-specific antiserum. Therefore, neither fdhD nor fdhE plays a structural role in the formation of FDH-PMS. Expression of a phi(fdhD-lacZ) operon fusion was decreased about threefold by aerobiosis but was indifferent to other effectors tested. It was unaffected by pfl, chlA, selA, and fnr mutations. Expression of a phi(fdhE-lacZ) operon fusion was slightly induced by nitrate. This induction, requiring the presence of functional chl and fnr alleles, was mediated via nitrate metabolism. Transcription of phi(fdhE-lacZ) fusion was fully dependent on wild-type sel alleles. This might suggest the participation of fdhE in the synthesis of the selenopolypeptide of FDH-PMS.
Schlindwein
C
C
Unité Mixte 24 du Centre National de la Recherche Scientifique, Institut National des Sciences Appliquées de Lyon, France.
Giordano
G
G
Santini
C L
CL
Mandrand
M A
MA
D003001:Cloning, Molecular / Q000379:methods; D004251:DNA Transposable Elements; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005560:Formate Dehydrogenases / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D005838:Genotype; D009154:Mutation; D010101:Oxygen Consumption; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D013604:T-Phages / Q000235:genetics
D004251:DNA Transposable Elements; D005560:Formate Dehydrogenases; D012321:DNA-Directed RNA Polymerases