The p53 nuclear localisation signal is structurally linked to a p34cdc2 kinase motif.
We have identified a region of human p53 protein with striking homology to a sequence motif on Simian Virus 40 T antigen which includes the nuclear localisation signal. Mutation of basic amino acid residues in this region of p53 (residues 312 to 323; SSSPQPKKKP) compromises transport of p53 protein to the nucleus. The sequence functions efficiently as a nuclear localisation signal when fused to E. coli beta galactosidase. Serine 315 within this p53 structural motif is phosphorylated in vitro by the cell cycle kinase p34cdc2. Thus in both T antigen and p53, nuclear localisation signal and p34cdc2 kinase acceptor residue map to a contiguous region of primary amino acid sequence.
Addison
C
C
Cell Proliferation Laboratory, Marie Curie Cancer Research Institute, Oxted, Surrey, UK.
Jenkins
J R
JR
Stürzbecher
H W
HW
D000595:Amino Acid Sequence; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D016203:CDC2 Protein Kinase; D002460:Cell Line; D006801:Humans; D008969:Molecular Sequence Data; D015513:Oncogene Proteins / Q000235:genetics*; D010750:Phosphoproteins / Q000235:genetics*; D011494:Protein Kinases / Q000235:genetics*; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013539:Simian virus 40 / Q000235:genetics / Q000276:immunology; D014162:Transfection; D016159:Tumor Suppressor Protein p53
D000952:Antigens, Polyomavirus Transforming; D015513:Oncogene Proteins; D010750:Phosphoproteins; D016159:Tumor Suppressor Protein p53; D011494:Protein Kinases; D011505:Protein-Tyrosine Kinases; D016203:CDC2 Protein Kinase