Immunoblotting reactivity of human sera from various sources against purified Epstein-Barr virus.
The immunoblotting technique was used to analyse polypeptides of purified Epstein-Barr virus reacting with antibodies present in sera from clinically healthy individuals, from patients with infectious mononucleosis (IM) or AIDS, and from renal transplant recipients. Polypeptides with molecular sizes in the range of 40-290 kDa were detected. The 47- and 160-kDa nucleocapsid polypeptides, as well as the 72-, 74-, 140-, 220- and 290-kDa membrane polypeptides were the major viral proteins detected in the sera. Sera from clinically healthy individuals contained antibodies directed against all EBV membrane and nucleocapsid antigens. Sera from renal transplant recipients, from patients with IM and from patients with AIDS failed to react with certain nucleocapsid and membrane antigens; in particular, sera from AIDS patients and renal transplant recipients did not react with the 220-kDa polypeptide, one of the major membrane antigens, while sera from subjects with IM and from healthy individuals did. A high proportion of sera from patients with IM (38% vs 5% of clinically healthy individuals and 0-5% of the AIDS patients and renal transplant recipients) reacted with a 42-kDa polypeptide, suggesting its possible role in acute EBV infection.
Ngou
J
J
Laboratoire de Développement et de Production, Centre Régional de Transfusion Sanguine, Montpellier, France.
Segondy
M
M
Graafland
H
H
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology; D000914:Antibodies, Viral; D004854:Herpesvirus 4, Human / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D015151:Immunoblotting; D007244:Infectious Mononucleosis / Q000276:immunology; D016030:Kidney Transplantation / Q000276:immunology; D008970:Molecular Weight; D014764:Viral Proteins / Q000276:immunology / Q000302:isolation & purification*
D000914:Antibodies, Viral; D014764:Viral Proteins