title: An immunogold assay system for the detection of antigen or antibody.
abstract: A colloidal gold-based semi-quantitative manual immunoassay method for the detection of antibody or antigen has been developed. The colloidal gold particles are coated with an organic reagent which, in turn, is attached to the antibody by covalent bonds. The antibody or the antigen are immobilized on simple chromatography paper. The paper strips are developed with an appropriate immunogold reagent in a test tube in the presence of urine (Pregnancy or Ovulation test) or serum (Rubella test). The mixture migrates up the strips towards the test band. A purple band develops which indicates the presence of the corresponding antigen (Pregnancy, Ovulation test) or antibody (Rubella test). In this format, 50 mIU or more of hCG in urine can be detected in 5 minutes or less, and antibody to Rubella virus at an HAI titer equivalent of 8 or above in serum can be detected in 10 minutes or less. These test kits, known as Result Plus test systems, are simple, rapid, highly sensitive, and require no instruments to perform.
authors.affiliation: Ortho Diagnostic Systems Inc., Raritan, New Jersey 08869.
authors.forename: U R
authors.initials: UR
authors.lastname: Chakraborty
authors.forename: N
authors.initials: N
authors.lastname: Black
authors.forename: H G
authors.initials: HG
authors.lastname: Brooks
authors.forename: C
authors.initials: C
authors.lastname: Campbell
authors.forename: K
authors.initials: K
authors.lastname: Gluck
authors.forename: F
authors.initials: F
authors.lastname: Harmon
authors.forename: L
authors.initials: L
authors.lastname: Hollenbeck
authors.forename: S
authors.initials: S
authors.lastname: Lawler
authors.forename: S
authors.initials: S
authors.lastname: Levison
authors.forename: D
authors.initials: D
authors.lastname: Mochnal
mesh_terms: D000914:Antibodies, Viral / Q000032:analysis; D006063:Chorionic Gonadotropin / Q000652:urine; D005260:Female; D006048:Gold Colloid, Radioactive; D006801:Humans; D010061:Ovulation Detection / Q000379:methods; D011247:Pregnancy; D011863:Radioimmunoassay / Q000379:methods*; D012412:Rubella virus / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D006063:Chorionic Gonadotropin; D006048:Gold Colloid, Radioactive