Localization of gene for human syndecan, an integral membrane proteoglycan and a matrix receptor, to chromosome 2.
Syndecan, a cell surface proteoglycan, is an integral membrane protein acting as a receptor for the extracellular matrix. For chromosomal localization of the human syndecan gene, a panel of mouse-human somatic cell hybrids was analyzed by Southern blotting using the cDNA probe for human syndecan. The hybrids were karyotyped at the time of DNA extraction. A band corresponding to the human syndecan gene in Southern blots was found only in a hybrid cell line containing human chromosome 2. This hybrid was subcloned and its subclones were analyzed by Southern blotting and karyotyped. Subclones carrying human chromosome 2 contained the syndecan gene, while subclones not carrying this chromosome did not. The human syndecan gene is thus assigned to chromosome 2.
Ala-Kapee
M
M
Department of Genetics, University of Helsinki, Finland.
Nevanlinna
H
H
Mali
M
M
Jalkanen
M
M
Schröder
J
J
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D002999:Clone Cells; D015342:DNA Probes; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D006801:Humans; D006822:Hybrid Cells; D007621:Karyotyping; D008562:Membrane Glycoproteins / Q000235:genetics*; D051379:Mice; D011509:Proteoglycans / Q000235:genetics*; D011956:Receptors, Cell Surface / Q000235:genetics*; D053667:Syndecans
D015342:DNA Probes; D016326:Extracellular Matrix Proteins; D008562:Membrane Glycoproteins; D011509:Proteoglycans; D011956:Receptors, Cell Surface; D053667:Syndecans; C052131:extracellular matrix receptor