Detection of human papillomavirus DNA in cervical lesions by in situ hybridization using biotinylated DNA probes.
In situ hybridization (ISH) for human papillomavirus (HPV)-6, -11, -16, -18, and -31 DNA was performed on 615 formalin-fixed paraffin-embedded cervical biopsies using biotinylated DNA probes. Results were obtained from 584 samples with 266 (45.5%) containing HPV-DNA sequences. Ninety percent of condyloma acuminatum specimens were positive for HPV-DNA with 18 of 19 positive cases containing HPV-6 or -11 DNA. The detection rate of HPV in cervical intraepithelial neoplasia (CIN) lesions was 50.6% (239 of 472), while only 8 of 91 (8.9%) cervical biopsies considered to be histologically normal or with minimal dysplasia contained HPV-DNA as demonstrated by ISH. The prevalence of HPV-16, -18, and/or -31 DNA increased with the severity of the lesions, with 20 of 20 (100%) positive CIN-III lesions containing these viral types compared with 102 of 157 (65.0%) positive CIN-I lesions. ISH with biotinylated DNA probes appears helpful in identifying lesions containing higher risk viral strains.
Amortegui
A J
AJ
Department of Pathology, Magee-Womens Hospital, Pittsburgh, Pennsylvania 15213.
Meyer
M P
MP
McIntyre-Seltman
K
K
Locker
J
J
D001706:Biopsy; D001710:Biotin; D012777:Cottontail rabbit papillomavirus / Q000302:isolation & purification*; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D009693:Nucleic Acid Hybridization; D014412:Tumor Virus Infections / Q000382:microbiology* / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000382:microbiology* / Q000473:pathology
D015344:DNA Probes, HPV; D004279:DNA, Viral; D001710:Biotin