title: Enhanced translational utilization of chloroplast ribosomal protein mRNAs from two AUG codons shown by site-directed mutation.
abstract: The cDNA for protein L12 of the chloroplast ribosome contains two ATG codons, both in consensus initiator context, at the beginning of its transit peptide coding region [Giese, K., & Subramanian, A. R. (1989) Biochemistry 28, 3525-3529]. Due to the location in the transit peptide, translational start from either ATG codon would yield the same mature protein after transport into chloroplasts and N-terminal cleavage. To test whether this arrangement, also found in the cDNAs of several other chloroplast ribosomal proteins, could enhance the utilization of mRNA, we constructed a chimeric gene containing the 5' part of the L12 cDNA fused to the neomycin phosphotransferase gene. A frameshift that would prematurely terminate the translation from the first AUG codon was introduced into the construct by site-directed mutagenesis. Transcription-translation of the fusion gene in vitro and its expression in vivo in spinach protoplasts showed that protein synthesis occurs from both initiation codons: at 70-80% of the total level from the first and 20-30% from the second. The results thus show that (1) eukaryotic ribosomes can initiate to a significant level from a downstream AUG codon and (2) the occurrence of two in-frame initiation codons enhances translational efficiency.
authors.affiliation: Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann, Berlin, FRG.
authors.forename: K
authors.initials: K
authors.lastname: Giese
authors.forename: A R
authors.initials: AR
authors.lastname: Subramanian
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002736:Chloroplasts / Q000378:metabolism*; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D004251:DNA Transposable Elements; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D015345:Oligonucleotide Probes; D010944:Plants / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits; D015183:Restriction Mapping; D012156:Reticulocytes / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics*
chemical_list: D003062:Codon; D004251:DNA Transposable Elements; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D012269:Ribosomal Proteins; C016700:ribosomal protein L7-L12