Effects of parathyroid hormone, calcitonin, and dibutyryl-cyclic AMP on osteoclast area in cultured chick tibia.
Slices of osteoclast-enriched endosteal surfaces of 3 week chick tibia were cultured for 1-3 days. Osteoclasts on the bone surface were made visible by acridine orange fluorescence. Osteoclast area was measured by image analysis. Parathyroid hormone (PTH) caused osteoclasts to increase in area about 40%, and calcitonin (CT) caused a decrease in area also of about 40%. Subsequent addition of dibutyryl cyclic AMP to PTH- or to CT-treated cells resulted in a further change of 40 and 30%, respectively. Application of the cyclic AMP analog alone had no effect. All responses were rapid, occurring in 2-4 minutes.
Pandalai
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Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
Gay
C V
CV
D000165:Acridine Orange; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002116:Calcitonin / Q000494:pharmacology*; D002645:Chickens; D046508:Culture Techniques; D010010:Osteoclasts / Q000187:drug effects*; D010281:Parathyroid Hormone / Q000494:pharmacology*; D013977:Tibia / Q000187:drug effects
D010281:Parathyroid Hormone; D003994:Bucladesine; D002116:Calcitonin; D000165:Acridine Orange