Eicosanoid synthesis and ornithine decarboxylase activity in mammary tumors of rats fed varying levels and types of N-3 and/or N-6 fatty acids.
Virgin female Sprague-Dawley rats (50 days of age) were administered a single intragastric 10 mg dose of 7,12-dimethylbenz(a) anthracene (DMBA). Three weeks later they were placed on diets containing either 20% corn oil (CO), 20% primrose oil (PO), 20% black currant seed oil (BCO), 20% borage oil (BO), 15% menhaden oil plus 5% corn oil (15% MO + 5% CO), 10% menhaden oil plus 10% corn oil (10% MO + 10% CO), 5% menhaden oil plus 15% corn oil (5% MO + 15% CO) or 10% menhaden oil plus 10% borage oil (10% MO + 10% BO). Incidences of mammary tumors at 16 weeks post-DMBA were 80% in rats fed the CO diet, 84% in rats fed PO diet, 67% in rats fed BCO diet, 88% in rats fed BO diet, 60% in rats fed 15% MO + 5% CO diet, 67% in rats fed 10% MO + 10% CO diet, 83% in rats fed 5% MO + 15% CO diet, and 92% in rats fed 10% MO + 10% BO diet. Tumor multiplicity was lowest in PO-fed rats and highest in BO-fed rats. The tumor burden per tumor-bearing rat was lowest in rats fed the 15% MO + 5% CO, and 10% MO + 10% CO, diets and highest in those fed 20% BCO diet. Although body weight at 16 weeks post DMBA was not significantly different among the dietary groups, food intake was significantly greater in rats fed a diet containing 20% BO, or 5% MO + 15% CO.(ABSTRACT TRUNCATED AT 250 WORDS)
Bunce
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Department of Pharmacology and Toxicology, College of Pharmacy, University of Georgia, Athens 30602.
Abou-El-Ela
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D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D004041:Dietary Fats / Q000494:pharmacology; D015777:Eicosanoids / Q000096:biosynthesis*; D005227:Fatty Acids / Q000494:pharmacology*; D005260:Female; D008042:Linolenic Acids / Q000494:pharmacology; D008325:Mammary Neoplasms, Experimental / Q000201:enzymology / Q000378:metabolism*; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D010938:Plant Oils / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D017965:gamma-Linolenic Acid
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004041:Dietary Fats; D015777:Eicosanoids; D005227:Fatty Acids; D008042:Linolenic Acids; D010938:Plant Oils; D015127:9,10-Dimethyl-1,2-benzanthracene; D017965:gamma-Linolenic Acid; D009955:Ornithine Decarboxylase