Vasoactive intestinal contractor, a novel peptide, shares a common receptor with endothelin-1 and stimulates Ca2+ mobilization and DNA synthesis in Swiss 3T3 cells.
Vasoactive intestinal contractor peptide (VIC), a novel member of the endothelin family, stimulated a rapid increase in the intracellular Ca2+ concentration in fura-2-loaded Swiss 3T3 cells. Sequential addition of VIC and endothelin-1 (ET1) demonstrated the induction of both homologous and heterologous desensitization. VIC was as potent as ET1 in displacing the binding of 125I-ET1 and in stimulating mitogenesis in Swiss 3T3 cells. These findings suggest that VIC and ET1 share a common receptor in Swiss 3T3 cells.
Fabregat
I
I
Imperial Cancer Research Fund, London, U.K.
Rozengurt
E
E
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D010455:Peptides / Q000378:metabolism* / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017466:Receptors, Endothelin
D007328:Insulin; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D011956:Receptors, Cell Surface; D017466:Receptors, Endothelin; C059568:vasoactive intestinal constrictor; D004815:Epidermal Growth Factor; D004247:DNA; D002118:Calcium