Increase of GABA-stimulated diazepam binding after lipid methylation in membrane preparations from rat brain.
Incubation of membrane preparations from rat brain with S-adenosyl-L-methionine resulted in methylation of the lipid fraction. Neither [3H]diazepam nor [3H]muscimol (a gamma-aminobutyric acid (GABA) agonist) binding was affected by this incubation. In contrast, when [3H]diazepam binding was stimulated by GABA, the methylation caused both a decrease of the minimal GABA concentration needed to produce its effect (10(-9) M instead of 10(-7) M) and an enhancement (from 36 to 66% over basal) of the GABA-stimulated [3H]diazepam binding.
Benistant
C
C
Unité INSERM 205, Laboratoire de Chimie Biologique, INSA, Villeurbanne, France.
Rey
C
C
Fonlupt
P
P
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D003975:Diazepam / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D008745:Methylation; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011963:Receptors, GABA-A / Q000378:metabolism*; D012436:S-Adenosylmethionine; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
D008563:Membrane Lipids; D010743:Phospholipids; D011963:Receptors, GABA-A; D005680:gamma-Aminobutyric Acid; D012436:S-Adenosylmethionine; D003975:Diazepam