The multiplication of transmissible gastroenteritis viruses in several cell lines originated from porcine kidney and effects of trypsin on the growth of the viruses.
Plaque formation, replication and related cytopathic function of 9 strains of transmissible gastroenteritis (TGE) virus were examined in primary cells and cell lines such as CPK, IB-RS-2, ESK, and PK-15 originated from porcine kidney and the effects of trypsin on the replication of TGE virus were examined in CPK cells. All strains produced a cytopathic effect and grew well in CPK cells as well as in primary porcine kidney cells. The effect of trypsin on the plaque formation was different from strains. The number of plaques produced by strains TO-163, Ukiha and Niigata increased from 2.6 to 3.52 times when trypsin was present in the medium during incubation at 37 degrees C for 1 hr after adsorption of the virus at 4 degrees C for 2 hr. The plaque sizes of TO-163, h-5, Ukiha and Niigata became larger from 1.4 to 1.7 times, when trypsin was present in the agar MEM overlay.
Honda
E
E
Department of Veterinary Microbiology, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.
Takahashi
H
H
Okazaki
K
K
Minetoma
T
T
Kumagai
T
T
D002460:Cell Line; D003332:Coronaviridae / Q000254:growth & development*; D013045:Species Specificity; D005760:Transmissible gastroenteritis virus / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology; D014357:Trypsin / Q000494:pharmacology*; D010948:Viral Plaque Assay / Q000379:methods*; D014779:Virus Replication / Q000187:drug effects
D014357:Trypsin