Three-dimensional structure of the oligosaccharide terminus of globotriaosylceramide and isoglobotriaosylceramide in solution. A rotating-frame NOE study using hydroxyl groups as long-range sensors in conformational analysis by 1H-NMR spectroscopy.
Spatial structures of the oligosaccharide parts of globotriaosylceramide, Gal(alpha 1-4)Gal(beta 1-4)Glc(beta 1-1)Cer (Cer = ceramide) and isoglobotriaosylceramide, Gal(alpha 1-3)Gal(beta 1-4)Glc(beta 1-1)Cer were investigated in (C2H3)2SO solution by means of laboratory and rotating frame NOE, hydroxyl protons being used as long-range sensors defining the distance constraints. Both oligosaccharides were found to exist in more than one conformation interconverting rapidly on the NMR time scale. The conformation of the Gal(alpha 1-4)Gal(beta 1-4)Glc beta trisaccharide dissolved in 2H2O appeared to be the same as that of the corresponding part of the glycosphingolipid in (C2H3)2SO solution.
Poppe
L
L
Max-Planck-Institut für Medizinische Forschung, Heidelberg, Federal Republic of Germany.
Dabrowski
J
J
von der Lieth
C W
CW
Koike
K
K
Ogawa
T
T
D002236:Carbohydrate Conformation; D003903:Deuterium; D017666:Deuterium Oxide; D005915:Globosides; D006028:Glycosphingolipids; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008958:Models, Molecular; D009844:Oligosaccharides; D012996:Solutions; D014281:Trihexosylceramides; D014867:Water
D005915:Globosides; D006028:Glycosphingolipids; D009844:Oligosaccharides; D012996:Solutions; D014281:Trihexosylceramides; D014867:Water; C018549:globotriaosylceramide; C037097:isoglobotriaosylceramide; D003903:Deuterium; D017666:Deuterium Oxide