Murine cytotoxic T lymphocytes specific for herpes simplex virus type 1 recognize the immediate early protein ICP4 but not ICP0.
Vaccinia virus recombinants expressing the herpes simplex virus type 1 (HSV-1) genes encoding ICP0 or ICP4 were used to identify the precise target antigen(s) of murine anti-viral cytotoxic T lymphocytes (CTL) specific for the non-structural immediate early proteins. These studies revealed that HSV-1-specific CTL, restricted to class I major histocompatibility complex genes of the H-2k haplotype but not the H-2d or H-2b haplotypes, would lyse autologous cells expressing ICP4. HSV-1-specific CTL derived from various mice strains failed to lyse target cells expressing ICP0. Calculation of the frequencies of H-2k-restricted virus-specific CTL demonstrated that approximately a third of the total HSV-1-specific response was directed against ICP4. Immunization of mice with either recombinant vaccinia virus or transfected L cells expressing ICP4 induced HSV-1-specific lymphoproliferation and delayed hypersensitivity but CTLs were not induced. More importantly, such immunized animals were unable to resist or control a subsequent challenge with virulent HSV-1.
Martin
S
S
Department of Microbiology and Nutrition Research, Upjohn Company, Kalamazoo, Michigan 49001.
Zhu
X X
XX
Silverstein
S J
SJ
Courtney
R J
RJ
Yao
F
F
Jenkins
F J
FJ
Rouse
B T
BT
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D015153:Blotting, Western; D016827:CD8 Antigens; D006183:H-2 Antigens / Q000276:immunology; D006561:Herpes Simplex / Q000517:prevention & control; D006968:Hypersensitivity, Delayed / Q000276:immunology; D017874:Immediate-Early Proteins; D066298:In Vitro Techniques; D007739:L Cells; D008213:Lymphocyte Activation; D051379:Mice; D018139:Simplexvirus / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D044767:Ubiquitin-Protein Ligases; D014614:Vaccines, Synthetic; D014616:Vaccinia virus; D014764:Viral Proteins / Q000276:immunology*; D054334:Viral Regulatory and Accessory Proteins / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens; D006183:H-2 Antigens; D017874:Immediate-Early Proteins; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C048925:herpes simplex virus, type 1 protein ICP4; D044767:Ubiquitin-Protein Ligases; C051804:Vmw110 protein, Human herpesvirus 1