title: Detection of microbial trypsin-like enzymes by use of an agar gel.
abstract: Certain oral micro-organisms associated with periodontal diseases elaborate trypsin-like enzymes. This study describes a single assay for these enzymes, based on the incorporation of the synthetic substrate N-alpha-benzoyl-DL-arginine-p-nitroanilide (BAPNA) into ion-agar gel. The system can provide quantitative or qualitative data within 24 h and is suitable for use in the dental clinic.
authors.affiliation: Naval Dental Research Institute, Great Lakes, Illinois 60088-5259.
authors.forename: E D
authors.initials: ED
authors.lastname: Pederson
authors.forename: B L
authors.initials: BL
authors.lastname: Lamberts
mesh_terms: D001120:Arginine / Q000031:analogs & derivatives; D001439:Bacteroides / Q000201:enzymology*; D001586:Benzoylarginine Nitroanilide; D002206:Capnocytophaga / Q000201:enzymology*; D003773:Dental Plaque / Q000382:microbiology; D006801:Humans; D009055:Mouth / Q000382:microbiology; D010518:Periodontitis / Q000382:microbiology*; D014357:Trypsin / Q000032:analysis*
chemical_list: D001586:Benzoylarginine Nitroanilide; D001120:Arginine; D014357:Trypsin