title: Further studies on substrates inducing metacyclogenesis in Trypanosoma cruzi.
abstract: Epimastigotes of Trypanosoma cruzi, Peru strain, incubated in Contreras' artificial triatomine urine transformed into metacyclic trypomastigotes when 10 mM L-glutamine, L-asparagine or D-fructose was added to the medium. Metacyclogenesis with these substrates was comparable to the percent metacyclic morphotype formation induced by L-proline and significantly greater than that stimulated by 10 mM D-glucose. Sodium acetate (10 mM) increased transformation induced by L-proline, and L-hydroxyproline (10 mM) increased transformation induced by D-fructose. Phosphoenolpyruvate (10 mM) inhibited L-proline-induced metacyclic trypomastigote stage formation. Three antimetabolites, azetidine 2-carboxylate (5 mM), malonic acid (1 mM), and desthiobiotin (5 mM), completely inhibited D-fructose-induced but not L-proline-induced transformation. The Costa Rica, Y, and CL strains of T. cruzi showed different patterns of percent metacyclogenesis with substrates that induce transformation in the Peru strain.
authors.affiliation: Department of Developmental and Cell Biology, University of California, Irvine 92717.
authors.forename: S M
authors.initials: SM
authors.lastname: Krassner
authors.forename: B
authors.initials: B
authors.lastname: Granger
authors.forename: P
authors.initials: P
authors.lastname: Phermsangngnam
authors.forename: T
authors.initials: T
authors.lastname: Le
authors.forename: V
authors.initials: V
authors.lastname: Linden
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001383:Azetidinecarboxylic Acid / Q000494:pharmacology; D001710:Biotin / Q000031:analogs & derivatives / Q000494:pharmacology; D050260:Carbohydrate Metabolism; D002454:Cell Differentiation; D003165:Complement System Proteins; D005947:Glucose / Q000378:metabolism; D006909:Hydroxyproline / Q000494:pharmacology; D008314:Malonates / Q000494:pharmacology; D011392:Proline / Q000378:metabolism*; D013045:Species Specificity; D014349:Trypanosoma cruzi / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism
chemical_list: D000596:Amino Acids; D008314:Malonates; D001383:Azetidinecarboxylic Acid; D001710:Biotin; C004749:desthiobiotin; D003165:Complement System Proteins; D011392:Proline; C030290:malonic acid; D005947:Glucose; D006909:Hydroxyproline