[Chronic hepatitis B: diagnostic value of hepatitis-B-virus DNA analysis in serum and liver tissue].
The diagnostic value of hybridization analyses for the detection of hepatitis B virus (HBV) DNA in serum and liver tissue was investigated in 79 patients with chronic liver disease. Active viral replication was demonstrated by the detection of HBeAg or HBV-DNA in serum or liver tissue. However, HBV-DNA was also detected in the absence of HBeAg in serum. No correlation was found between the presence of HBV-DNA in serum or liver tissue and clinico-chemical or histological disease. In HBsAg-negative patients no HBV-DNA was detected in serum or liver tissue. These data indicate that serological markers are sufficient for the diagnosis of HBV infection. However, detection of HBV-DNA in serum or active viral replication in liver tissue is important in selecting patients for antiviral therapy (e.g. interferon) and monitoring its efficacy.
Meier
P
P
Stadtspital Waid, Zürich.
Walter
E
E
Blum
H E
HE
Offensperger
W B
WB
Offensperger
S
S
Schmid
M
M
Gerok
W
W
D000293:Adolescent; D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006509:Hepatitis B / Q000382:microbiology*; D006513:Hepatitis B e Antigens / Q000032:analysis; D006515:Hepatitis B virus / Q000276:immunology / Q000648:ultrastructure*; D006521:Hepatitis, Chronic / Q000382:microbiology*; D006801:Humans; D008099:Liver / Q000648:ultrastructure; D008104:Liver Cirrhosis, Alcoholic / Q000175:diagnosis; D008113:Liver Neoplasms / Q000175:diagnosis; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D014779:Virus Replication
D004279:DNA, Viral; D006513:Hepatitis B e Antigens
Chronische Hepatitis B: Stellenwert des Nachweises von Hepatitis-B-Virus-DNA in Serum und Lebergewebe.