title: A study of the predominant genotypes of bovid herpesvirus 1 found in the U.K.
abstract: The genotypic classification of 84 U.K. isolates of bovid herpesvirus 1 was determined by the restriction endonuclease technique. Preliminary studies with six enzymes (EcoRI, HindIII, HpaI, BamHI, PstI and BstEII) showed that the principal genotypic variants, including the live vaccine strains used in the U.K., could be identified from the digestion patterns with just two endonucleases: HindIII and HpaI. Isolates from the 1960s were all categorised as genotype 2b. From 1977 onwards, genotype 1 has predominated in mainland Britain, with occasional type 2b isolates. Type 2b viruses were isolated from both respiratory and genital disease cases. There was no evidence of genotypes 2a or 3 in the U.K.
authors.affiliation: Central Veterinary Laboratory, Weybridge, Surrey, Great Britain.
authors.forename: S
authors.initials: S
authors.lastname: Edwards
authors.forename: H
authors.initials: H
authors.lastname: White
authors.forename: P
authors.initials: P
authors.lastname: Nixon
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000032:analysis*; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D005838:Genotype; D007242:Herpesvirus 1, Bovine / Q000145:classification*; D009710:Nucleotide Mapping; D015183:Restriction Mapping; D006113:United Kingdom
chemical_list: D004279:DNA, Viral; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; C024406:endodeoxyribonuclease HpaI; D015252:Deoxyribonucleases, Type II Site-Specific