Constitutive expression of HIV-1 tat protein in human Jurkat T cells using a BK virus vector.
The production and characterization of Jurkat cell lines that constitutively express functional human immune deficiency virus type 1 (HIV-1) tat protein, using a BK virus plasmid expression vector and HIV-1 tat cDNA, is described. An increased growth rate of these Jurkat-tat cell lines as compared with control cell lines was observed.
Caputo
A
A
Division of Human Retrovirology, Dana-Farber Cancer Institute, Boston, MA 02115.
Sodroski
J G
JG
Haseltine
W A
WA
D001739:BK Virus; D002455:Cell Division / Q000235:genetics; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis; D015870:Gene Expression / Q000235:genetics; D015696:Gene Products, tat / Q000096:biosynthesis*; D005822:Genetic Vectors; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D010957:Plasmids; D011120:Polyomavirus; D012367:RNA, Viral / Q000032:analysis; D013601:T-Lymphocytes / Q000378:metabolism*; D015534:Trans-Activators / Q000096:biosynthesis*; D014162:Transfection; D054322:tat Gene Products, Human Immunodeficiency Virus
D004279:DNA, Viral; D015696:Gene Products, tat; D012367:RNA, Viral; D015534:Trans-Activators; D054322:tat Gene Products, Human Immunodeficiency Virus