Human immunoglobulin G potentiates superoxide production induced by chemotactic peptides and causes degranulation in isolated human neutrophils.
Neutrophils are major cellular mediators of host defense and inflammation. They can be activated to produce superoxide and to release the contents of their granules to the extracellular space. We observed that monomeric human immunoglobulin G (IgG) sensitizes these cells to the chemotactic peptide N-formylmethionylleucylphenylalanine (fMLP). In cells submaximally stimulated by fMLP this enhancement was especially prominent. With saturating fMLP concentrations, the rate of O2- production was still about twice that in the control. No synergy with other activators (phorbol myristate acetate, concanavalin A) was observed. Binding of fMLP to the cells was decreased by IgG, resembling the effect of cytochalasin B. IgG did not induce O2- production on its own, but it stimulated degranulation of the neutrophils.
Aaku
E
E
Department of Medical Chemisty, University of Helsinki, Finland.
Sorsa
T
T
Wilkström
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M
D002118:Calcium / Q000378:metabolism; D056649:Cathepsin G; D002403:Cathepsins / Q000378:metabolism; D015550:Cell Degranulation; D003208:Concanavalin A / Q000494:pharmacology; D004357:Drug Synergism; D006801:Humans; D007074:Immunoglobulin G / Q000502:physiology*; D066298:In Vitro Techniques; D003012:Microbial Collagenase / Q000378:metabolism; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000493:pharmacokinetics*; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D012697:Serine Endopeptidases; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007074:Immunoglobulin G; D003208:Concanavalin A; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D002403:Cathepsins; D012697:Serine Endopeptidases; C534094:CTSG protein, human; D056649:Cathepsin G; D003012:Microbial Collagenase; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium