Polypeptide specificity of antiviral serum antibodies in children naturally infected with human rotavirus.
Reassortants between serotype 3 SA11 and serotype 6 NCDV rotaviruses were used to determine the relative amounts of serum-neutralizing antibody to VP4 and VP7 of serotype 3 SA11 rotavirus in children after natural rotavirus exposure. Sera from Ecuadorian children of a population-based study and sera from children of a hospital-based study in Germany (excluding diarrhea patients) demonstrated high titers of VP7-specific but only low titers of VP4-specific antibodies. In contrast, paired sera from German children hospitalized with a symptomatic primary rotavirus gastroenteritis demonstrated a titer increase to VP4 more frequently than to VP7 protein by neutralization test and immunoblotting. For these rotavirus patients, we provided, previously, direct evidence for the development of cross-neutralizing antibodies.
Brüssow
H
H
Nestlé Research Centre, Lausanne, Switzerland.
Offit
P A
PA
Gerna
G
G
Bruttin
A
A
Sidoti
J
J
D000914:Antibodies, Viral / Q000276:immunology* / Q000302:isolation & purification; D000956:Antigens, Viral; D002213:Capsid / Q000276:immunology; D036022:Capsid Proteins; D002675:Child, Preschool; D004484:Ecuador; D005860:Germany, West; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000276:immunology / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000276:immunology / Q000302:isolation & purification; D007223:Infant; D009500:Neutralization Tests; D012401:Rotavirus / Q000145:classification / Q000276:immunology*; D012400:Rotavirus Infections / Q000276:immunology*; D012703:Serotyping
D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus