Presentation of human minor histocompatibility antigens by HLA-B35 and HLA-B38 molecules.
Cytotoxic T lymphocyte (CTL) clones specific for human minor histocompatibility antigens (hmHAs) were produced from a patient who had been grafted with the kidneys from his mother and two HLA-identical sisters. Of eight CTL clones generated, four recognized an hmHA (hmHA-1) expressed on cells from the mother and sister 3 (second donor); two recognized another antigen (hmHA-2) on cells from the father, sister 2 (third donor), and sister 3; and the remaining two clones recognized still another antigen (hmHA-3) on cells from the father and sister 3. Panel studies revealed that CTL recognition of hmHA-1 was restricted by HLA-B35 and that of hmHA-2 and hmHA-3 was restricted by HLA-B38. The HLA-B35 restriction of the hmHA-1-specific CTL clones was substantiated by the fact that they killed HLA-A null/HLA-B null Hmy2CIR targets transfected with HLA-B35 but not HLA-B51, -Bw52, or -Bw53 transfected Hmy2CIR targets. These data demonstrated that the five amino acids substitutions on the alpha 1 domain between HLA-B35 and -Bw53, which are associated with Bw4/Bw6 epitopes, play a critical role in the relationship of hmHA-1 to HLA-B35 molecules. The fact that the hmHA-1-specific CTLs failed to kill Hmy2CIR cells expressing HLA-B35/51 chimeric molecules composed of the alpha 1 domain of HLA-B35 and other domains of HLA-B51 indicated that eight residues on the alpha 2 domain also affect the interaction of hmHA-1 and the HLA-B35 molecules.
Yamamoto
J
J
Department of Immunology, University of Tokyo, Japan.
Kariyone
A
A
Akiyama
N
N
Kano
K
K
Takiguchi
M
M
D000938:Antigen-Presenting Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002472:Cell Transformation, Viral; D003602:Cytotoxicity, Immunologic; D005260:Female; D015235:HLA-B Antigens / Q000276:immunology*; D015797:HLA-B35 Antigen / Q000276:immunology*; D059945:HLA-B38 Antigen; D006239:Haplotypes; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology; D008297:Male; D015778:Minor Histocompatibility Antigens / Q000276:immunology*; D010375:Pedigree; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D015235:HLA-B Antigens; D015797:HLA-B35 Antigen; D059945:HLA-B38 Antigen; D015778:Minor Histocompatibility Antigens