title: A DNA gyrase-binding site at the center of the bacteriophage Mu genome is required for efficient replicative transposition.
abstract: We have discovered a centrally located site that is required for efficient replication of bacteriophage Mu DNA and identified it as a strong DNA gyrase-binding site. Incubation of Mu DNA with gyrase and enoxacin revealed a cleavage site 18.1 kilobases from the left end of the 37.2-kilobase genome. Two observations indicate a role for the site in Mu replication: mutants of Mu, able to grow on an Escherichia coli gyrB host that does not allow growth of wild-type Mu, were found to possess single-base changes resulting in more efficient gyrase binding and cleavage at the site. Introduction of a 147-base-pair deletion that eliminated the site from a prophage inhibited the onset of Mu replication for greater than 1 hr after induction.
authors.affiliation: Department of Microbiology and Immunology, University of Colorado Health Sciences Center, Denver 80262.
authors.forename: M L
authors.initials: ML
authors.lastname: Pato
authors.forename: M M
authors.initials: MM
authors.lastname: Howe
authors.forename: N P
authors.initials: NP
authors.lastname: Higgins
mesh_terms: D010583:Bacteriophage mu / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D004261:DNA Replication; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D004251:DNA Transposable Elements; D004278:DNA, Superhelical / Q000502:physiology; D004279:DNA, Viral / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D015183:Restriction Mapping; D014779:Virus Replication
chemical_list: D004251:DNA Transposable Elements; D004278:DNA, Superhelical; D004279:DNA, Viral; D004250:DNA Topoisomerases, Type II