Isolation and characterization of a complementary DNA clone for an algal pre-apoplastocyanin.
We have isolated a cDNA clone for the Chlamydomonas reinhardtii pre-apoplastocyanin. The sequence contains codons for the complete pre-protein including a two-domain, lumen-targeting transit sequence and the mature apoprotein. The transit sequence (47 amino acids) is the shortest one described for chloroplast lumenal proteins, and like other C. reinhardtii lumen-targeting transit sequences appears to lack an uncharged amino-terminal domain usually present in plant lumen-directing sequences. The mature protein is deduced to be 98 amino acids in length and shows highest primary sequence similarity (74-76% identity) to other unicellular algal plastocyanins. Southern hybridization analysis of C. reinhardtii genomic DNA indicates the presence of a single nuclear gene, as is the case for all other plastocyanin genes characterized to date, although the algal gene might be interrupted. Codon usage in this gene reflects the high GC content of C. reinhardtii nuclear DNA, but is more highly biased than that found in the C. reinhardtii copper-repressible gene for the functionally equivalent pre-apocytochrome c552 (perhaps contributing to the more efficient synthesis in vivo of plastocyanin over cytochrome c552). The deduced physical properties of this plastocyanin are compared to those of the C. reinhardtii plastidic cytochrome c552.
Merchant
S
S
Department of Chemistry and Biochemistry, UCLA 90024-1569.
Hill
K
K
Kim
J H
JH
Thompson
J
J
Zaitlin
D
D
Bogorad
L
L
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002451:Cell Compartmentation; D002696:Chlamydomonas / Q000235:genetics*; D003001:Cloning, Molecular; D003062:Codon; D003574:Cytochrome c Group / Q000235:genetics; D004247:DNA / Q000235:genetics; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D010970:Plastocyanin / Q000235:genetics* / Q000378:metabolism; D011233:Precipitin Tests; D011498:Protein Precursors / Q000235:genetics
D003062:Codon; D003574:Cytochrome c Group; D010940:Plant Proteins; D011498:Protein Precursors; C083951:cytochrome c553; D004247:DNA; D010970:Plastocyanin; C022302:cytochrome C-552