title: Effect of colchicine on lysosomal structures in maturation-ameloblasts of the rat incisor.
abstract: The lysosomal systems in maturation-ameloblasts affected by colchicine were examined using trimetaphosphatase cytochemistry. Demineralized segments of rat incisor were incubated for trimetaphosphatase. At all time intervals, lysosomal structures exhibited reduced enzyme reactivity and were clustered in the Golgi region of the cell. Both ruffle-ended and smooth-ended ameloblasts maintained essentially normal morphology up to 4 h after colchicine injection, except for some migration of organelles. After 8 h, the ruffled border was markedly modified and the associated dense granular material was no longer present. Changes in the lysosomal system and ruffled border indicate interference by colchicine with a putative resorptive function of the maturation-ameloblasts.
authors.affiliation: Oral Biology Department, Faculty of Oral and Dental Medicine, Cairo University, Egypt.
authors.forename: A H
authors.initials: AH
authors.lastname: Salama
authors.forename: D R
authors.initials: DR
authors.lastname: Eisenmann
authors.forename: A E
authors.initials: AE
authors.lastname: Zaki
mesh_terms: D017766:Acid Anhydride Hydrolases; D000565:Ameloblasts / Q000187:drug effects / Q000648:ultrastructure*; D000818:Animals; D003078:Colchicine / Q000494:pharmacology*; D006651:Histocytochemistry; D007180:Incisor / Q000187:drug effects / Q000648:ultrastructure*; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D010744:Phosphoric Monoester Hydrolases; D017766:Acid Anhydride Hydrolases; C022768:trimetaphosphatase; D003078:Colchicine