Phosphatidyltransferase of Clostridium butyricum: specificity for diacylphosphoglycerides.
Phosphatidyltransferase from Clostridium butyricum, which catalyzes transfer of the phosphatidyl moiety of phosphatidylethanolamine (PE), phosphatidylglycerol (PG) or phosphatidylserine to primary alcohols such as glycerol, serine and ethanolamine, was tested for its ability to catalyze transfer of the plasmenyl moiety from plasmalogen analogs of PE or PG to glycerol or ethanolamine, respectively. The cell membrane of C. butyricum contains high proportions of these plasmalogens. When diacyl and plasmalogen forms of PE or PG were supplied as donors in equimolar amounts to membrane particles, the diacyl forms were the preferred donors by approx. 7 and 10 to 1, respectively. When the molar ratio of PE its plasmalogen was 1:3.3, the ratio of PG formed to its plasmalogen was 1:0.66. These results show that the enzyme(s) can catalyze transfer of both the diacyl and alkenyl acyl forms of glycerophospholipids, but the diacyl forms are used preferentially.
Morii
H
H
Department of Microbiology, University of Pennsylvania, School of Medicine, Philadelphia 19104-6076.
Goldfine
H
H
D002462:Cell Membrane / Q000378:metabolism; D003013:Clostridium / Q000201:enzymology*; D007700:Kinetics; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D010715:Phosphatidylglycerols / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism*; D010955:Plasmalogens / Q000378:metabolism; D013379:Substrate Specificity; D017855:Transferases (Other Substituted Phosphate Groups)
D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D010955:Plasmalogens; C020791:phosphatidal ethanolamines; D010770:Phosphotransferases; C064918:phosphatidyltransferase, Clostridium butyricum; D017855:Transferases (Other Substituted Phosphate Groups)