Stimulation of poly(ADP-ribose) synthesis by free radicals in C3H10T1/2 cells: relationship with NAD metabolism and DNA breakage.
We have studied the effect of H2O2 and O2- produced by xanthine and xanthine oxidase on NAD catabolism, poly(ADP-ribose) synthesis, and production of DNA single-strand breaks in C3H10T1/2 cells. The results show a correlation between the induction of DNA single-strand breaks, the decrease of NAD pool, and the accumulation of polymer. New techniques, based on affinity chromatography and reversed-phase high pressure liquid chromatography, have allowed an accurate determination of polymer contents and showed a 20-fold stimulation of polymer biosynthesis induced by active oxygen species. Inhibition experiments performed with 3-aminobenzamide have shown that the decrease in NAD levels after exposure of cells to active oxygen species was caused by stimulation of poly(ADP-ribosyl)ation and of another cellular process.
Lautier
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D
Laboratoire du métabolisme du poly(ADP-ribose), Centre hospitalier de l'Université Laval, Ste-Foy, Qué., Canada.
Poirier
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D
Boudreau
A
A
Alaoui Jamali
M A
MA
Castonguay
A
A
Poirier
G
G
D000469:Alkaline Phosphatase; D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D002999:Clone Cells; D004249:DNA Damage; D005609:Free Radicals; D005720:Gamma Rays; D006861:Hydrogen Peroxide / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D051379:Mice; D009243:NAD / Q000378:metabolism*; D009702:Nucleoside Diphosphate Sugars / Q000096:biosynthesis*; D010727:Phosphoric Diester Hydrolases; D011064:Poly Adenosine Diphosphate Ribose / Q000096:biosynthesis*; D013481:Superoxides / Q000378:metabolism; D014316:Tritium; D019820:Xanthine; D014969:Xanthine Oxidase / Q000378:metabolism; D014970:Xanthines / Q000378:metabolism
D001549:Benzamides; D005609:Free Radicals; D009702:Nucleoside Diphosphate Sugars; D014970:Xanthines; D009243:NAD; D014316:Tritium; D013481:Superoxides; D019820:Xanthine; D011064:Poly Adenosine Diphosphate Ribose; C025160:3-aminobenzamide; D006861:Hydrogen Peroxide; D014969:Xanthine Oxidase; D000469:Alkaline Phosphatase; D010727:Phosphoric Diester Hydrolases