[A basic study of cytotoxic effect of tumor necrosis factor-alpha on the hepatoma cell line, PLC/PRF/5].
In order to investigate the effect of tumor necrosis factor (recombinant TNF-alpha) on hepatoma, we assessed the antitumor activity of TNF-alpha on hepatoma cell line, PLC/PRF/5 in vitro, and combined effects of interferon-gamma (IFN-gamma), that is thought to increase TNF-receptors. TNF-receptors. TNF-alpha for itself, had no significant effect on DNA synthesis and viability, and the production of HBsAg from PLC/PRF/5. On the other hand, treatment of IFN-gamma- combined with TNF-alpha inhibited DNA synthesis and production of HBsAg. The decrease of viability of PLC/PRF/5 was also found in this combination treatment. According to these findings, antitumor activity of TNF-alpha had no effect on PLC/PRF/5 in itself, but was significantly enhanced when treated with IFN-gamma at the same time.
Takayama
H
H
Department of Internal Medicine, Maebashi Red Cross Hospital.
Takagi
H
H
Saito
S
S
Ojima
T
T
Iizuka
H
H
Katakai
S
S
Yamada
S
S
Kobayashi
S
S
D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002470:Cell Survival; D004357:Drug Synergism; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology; D008113:Liver Neoplasms / Q000473:pathology*; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D007370:Interferon Type I; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha