Direct oxidation of lymphocytes by chromic acid does not induce blastogenesis.
Blastogenic and cytotoxic effects of hexavalent chromium were evaluated by direct, 2 and 20 min oxidation of lymphocytes by 10.0 to 0.0005 mM CrO3 at 0 degrees C. Oxidized cells exhibited concentration-dependent cytotoxicity and the inhibition of tritiated thymidine incorporation rates. When lymphocytes were oxidized first by 1.0 mM periodic acid (H5IO6) and thereafter by 1.0 mM CrO3, the viability and [3H]-TdR incorporation rates of sequentially oxidized cells were identical to the corresponding indicators of lymphocytes oxidized only by CrO3. The reversal of the oxidation sequence restored [3H]-TdR incorporation to control levels and increased cell survival. It is therefore concluded that direct interaction of hexavalent CrO3 with plasma membrane of lymphocytes results in concentration-dependent cytotoxicity and the inhibition of [3H]-TdR incorporation, but it does not induce blastogenesis.
Malinin
G I
GI
Department of Physics, Georgetown University, Washington, DC 20057.
Hornicek
F J
FJ
Malinin
T I
TI
D000818:Animals; D002478:Cells, Cultured; D002840:Chromates / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism*; D051379:Mice; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000494:pharmacology
D002840:Chromates; D010504:Periodic Acid; C034944:chromic acid