DNaseI hypersensitive sites at the 3' end of the human apolipoprotein B gene.
The chromatin structure of the 3' end of the human apolipoprotein B gene has been examined. Two DNaseI hypersensitive sites were present in nuclei from liver-derived HepG2 cells and intestine-derived CaCo-2 cells, in which the apo-B gene is transcriptionally active, but were absent from HeLa cells, where the gene is not expressed, and from free DNA. The region in a segment enriched in recognition sites for topoisomerase II and known to participate in anchoring the 3' end of the gene to the nuclear matrix. The second DNaseI hypersensitive site resided in the 3' untranslated portion of the gene. Furthermore, nucleosomes were present along a 1.4-kilobase (HindIII-BamHI) segment of DNA containing the two 3' DNaseI hypersensitive sites, and a static array of nucleosomes was present along the A/T-rich hypervariable region.
Levy-Wilson
B
B
Gladstone Foundation Laboratories for Cardiovascular Disease, Department of Pharmaceutical Chemistry, University of California, San Francisco 94140-0608.
D001055:Apolipoproteins B / Q000235:genetics*; D015139:Blotting, Southern; D002460:Cell Line; D002874:Chromosome Mapping; D004264:DNA Topoisomerases, Type I / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000494:pharmacology; D005786:Gene Expression Regulation; D006801:Humans; D015530:Nuclear Matrix / Q000648:ultrastructure; D009707:Nucleosomes / Q000648:ultrastructure; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping
D001055:Apolipoproteins B; D009707:Nucleosomes; D003850:Deoxyribonuclease I; D004264:DNA Topoisomerases, Type I