Production of interleukin-1 beta by periodic acid-oxidized human peripheral blood mononuclear cells.
Interleukin-1 (IL-1) production by periodic acid (H5IO6)-oxidized human peripheral blood mononuclear (PBMN) cells was assessed by the thymocyte co-mitogenesis assay. Maximum IL-1 levels (approximately 1.2 U/ml) in the conditioned media of PBMN cells were registered within the first 24 hrs post-oxidation, whereas no IL-1 was detected in the media from 24 hrs control cultures. Thymocyte proliferation, driven by periodic acid-induced IL-1, was abolished by an antibody to IL-1 alpha and IL-1 beta. Quantitative analysis of IL-1-containing medium by radioimmunoassay (RIA) indicated that IL-1 beta comprised about 80% of total IL-1. Partial characterization of H5IO6-induced IL-1 beta indicated that it was identical to IL-1 produced by lipopolysaccharide-stimulated macrophages. It is concluded that oxidation of human PBMN cells by H5IO6 triggers synthesis and release of IL-1, most of which was in its IL-1 beta form.
Malinin
G I
GI
Physics Department, Georgetown University, Washington, DC 20057.
Hornicek
F J
FJ
Malinin
T I
TI
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D007375:Interleukin-1 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D010084:Oxidation-Reduction; D010504:Periodic Acid; D011863:Radioimmunoassay; D013601:T-Lymphocytes / Q000378:metabolism
D007375:Interleukin-1; D010504:Periodic Acid