title: Cloning and characterization of a Drosophila serotonin receptor that activates adenylate cyclase.
abstract: Using a strategy based on nucleotide sequence homology between genes encoding receptors that interact with guanine nucleotide-binding proteins, we have isolated Drosophila genomic and cDNA clones encoding a functional serotonin receptor (5HT-dro receptor). This protein is expressed predominantly in Drosophila heads and exhibits highest homology with the human 5HT1A receptor. The predicted structure of the 5HT-dro receptor reveals two unusual features: (i) eight putative transmembrane domains instead of the expected seven and (ii) a Gly-Ser repeat that is a potential glycosaminoglycan attachment site. When stably introduced into mouse NIH 3T3 cells, the 5HT-dro receptor activates adenylate cyclase in response to serotonin and is inhibited by serotonin receptor antagonists such as dihydroergocryptine. The 5HT-dro receptor or closely related receptors might be responsible for the serotonin-sensitive cyclase that has been suggested to play a role in learning and modulation of circadian rhythm in a number of invertebrate systems.
authors.affiliation: Laboratoire de Génétique Moléculaire des Eucaryotes dú Centre National de la Recherche Scientifique, Unité 184 de Génie Génétique et de Biologie Moléculaire, Faculté de Médecine, Strasbourg, France.
authors.forename: P
authors.initials: P
authors.lastname: Witz
authors.forename: N
authors.initials: N
authors.lastname: Amlaiky
authors.forename: J L
authors.initials: JL
authors.lastname: Plassat
authors.forename: L
authors.initials: L
authors.lastname: Maroteaux
authors.forename: E
authors.initials: E
authors.lastname: Borrelli
authors.forename: R
authors.initials: R
authors.lastname: Hen
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000378:metabolism; D004247:DNA / Q000235:genetics; D004789:Enzyme Activation; D005796:Genes; D006025:Glycosaminoglycans / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D011985:Receptors, Serotonin / Q000235:genetics*; D012701:Serotonin / Q000494:pharmacology; D014162:Transfection
chemical_list: D006025:Glycosaminoglycans; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D011985:Receptors, Serotonin; D012701:Serotonin; D004247:DNA; D000242:Cyclic AMP; D000262:Adenylyl Cyclases