The v-rel oncogene product is complexed with cellular proteins including its proto-oncogene product and heat shock protein 70.
The oncogene product, pp59v-rel, of avian reticuloendotheliosis virus (REV-T) is complexed in the cytosol of REV-T transformed lymphoid cells with cellular proteins. Monoclonal antibodies and antisera directed against different regions of pp59v-rel coimmunoprecipitate five cellular proteins (p124, p115, p75, p70, and p40) in addition to pp59v-rel. Cellular proteins with the same apparent molecular mass also copurify with pp59v-rel during sequential Sephacryl S200 and immunoaffinity chromatography. Antisera directed against the most abundant cellular protein in the complex, pp40, coimmunoprecipitate pp59v-rel and several cellular proteins with the same apparent molecular mass. The 75-kDa protein in the pp59v-rel complex is the product of c-rel proto-oncogene and is weakly phosphorylated. In MSB-1 cells this protein is not detectably phosphorylated or associated with cellular proteins. The 70-kDa protein in the pp59v-rel containing cytosolic complex is the constitutive form of avian heat shock protein 70 (HSC70). The p70 protein coimmunoprecipitates and copurifies with pp59v-rel using antisera directed against pp59v-rel and coimmunoprecipitates with antisera specific for pp40. The p70 isolated from immune complexes containing pp59v-rel shares V8 protease fragments with HSC70.
Lim
M Y
MY
Department of Microbiology, University of Texas, Austin 78712.
Davis
N
N
Zhang
J Y
JY
Bose
H R
HR
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002645:Chickens; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005822:Genetic Vectors; D006360:Heat-Shock Proteins / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D020572:Oncogene Proteins v-rel; D010449:Peptide Mapping; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000302:isolation & purification / Q000378:metabolism*; D020569:Proto-Oncogene Proteins c-rel; D011519:Proto-Oncogenes; D015183:Restriction Mapping; D001356:Reticuloendotheliosis virus / Q000235:genetics / Q000378:metabolism; D015682:Retroviridae Proteins, Oncogenic / Q000302:isolation & purification / Q000378:metabolism*
D006360:Heat-Shock Proteins; D020572:Oncogene Proteins v-rel; D010750:Phosphoproteins; D011518:Proto-Oncogene Proteins; D020569:Proto-Oncogene Proteins c-rel; D015682:Retroviridae Proteins, Oncogenic; D011505:Protein-Tyrosine Kinases