title: The metaphase specific phosphorylation of HMG I.
abstract: In vivo labelling of HeLa cells arrested in metaphase with [32P]-phosphate and in vitro phosphorylation of HMG I with the partially purified growth associated H1 kinase was used to study metaphase specific phosphorylation of HMG I. It was found that threonine 53 and 78 became phosphorylated. These amino acids are embedded in respectively the sequence PTPKR and TPGRK which are similar to the sequences phosphorylated by the growth associated H1 kinase.
authors.affiliation: Department of Biochemistry, University of Oslo, Norway.
authors.forename: T
authors.initials: T
authors.lastname: Lund
authors.forename: S G
authors.initials: SG
authors.lastname: Laland
mesh_terms: D000595:Amino Acid Sequence; D006367:HeLa Cells; D006609:High Mobility Group Proteins / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008677:Metaphase; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010766:Phosphorylation; D006656:Protamine Kinase / Q000378:metabolism; D013820:Thermolysin
chemical_list: D006609:High Mobility Group Proteins; D006657:Histones; D006656:Protamine Kinase; D013820:Thermolysin