Identification of malignant cell clones in radio-induced murine thymic lymphomas by viral and cellular probes.
The role of B ecotropic recombinant retroviruses in the emergence and the progression of radio-induced thymic lymphomas was evaluated by analyzing the cell populations present in nine primary and in in vivo propagated tumors. For this, tumor DNAs were analyzed by the Southern method using probes specific for newly acquired proviral sequences, T-cell receptor beta-chain, and immunoglobulin heavy chain genes. Our results show that primary radio-induced tumors are composed of several tumoral cell clones but do not support that malignant cell transformation and proliferation are conferred, solely, by the newly acquired ecotropic recombinant retroviral sequences.
Astier-Gin
T
T
INSERM U.117, Bordeaux, France.
Legrand
E
E
Baylac-Kalabokias
H
H
Guillemain
B
B
D000818:Animals; D002999:Clone Cells; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / Q000235:genetics; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D008223:Lymphoma / Q000235:genetics* / Q000382:microbiology; D051379:Mice; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation; D009381:Neoplasms, Radiation-Induced / Q000235:genetics* / Q000382:microbiology; D010641:Phenotype; D011948:Receptors, Antigen, T-Cell / Q000235:genetics; D013601:T-Lymphocytes / Q000473:pathology*; D013953:Thymus Neoplasms / Q000235:genetics* / Q000382:microbiology
D015342:DNA Probes; D004273:DNA, Neoplasm; D004279:DNA, Viral; D007143:Immunoglobulin Heavy Chains; D011948:Receptors, Antigen, T-Cell