Adrenal pheochromocytoma PC12h cells respond to pituitary adenylate cyclase activating polypeptide.
An adrenal pheochromocytoma cell line, PC12h, was found to respond to a novel hypothalamic neuropeptide, Pituitary Adenylate Cyclase Activating Polypeptide (PACAP). The cells elevated both intracellular and extracellular cAMP levels on stimulation by PACAP, whereas they showed little response to VIP which is structurally related to PACAP. Using [125I]PACAP27 (a shorter form of the peptide) and [125I]VIP, we found large amounts of specific binding sites for PACAP but few binding sites for VIP in PC12h cells. These results indicate that PC12h cells respond to PACAP via a specific PACAP receptor.
Watanabe
T
T
Tsukuba Research Laboratories, Takeda Chemical Industries, Ltd., Ibaraki, Japan.
Ohtaki
T
T
Kitada
C
C
Tsuda
M
M
Fujino
M
M
D000310:Adrenal Gland Neoplasms; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism*; D007700:Kinetics; D009479:Neuropeptides / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D010673:Pheochromocytoma; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D051381:Rats; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism / Q000494:pharmacology
C495403:Adcyap1 protein, rat; D009479:Neuropeptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D014660:Vasoactive Intestinal Peptide; D000242:Cyclic AMP