title: N-methyl-D-aspartate-evoked calcium uptake by kitten visual cortex maintained in vitro.
abstract: As a functional measure of NMDA receptor effectiveness in kitten striate cortex, the uptake of 45Ca by visual cortical slices was measured after 2 minute bath applications of N-methyl-D-aspartate (NMDA). Significant Ca uptake occurred in response to 12.5-100 microM NMDA in slices prepared from visual cortex of normal animals aged 28-48 days. Basal uptake (in the absence of NMDA) was increased and evoked uptake was decreased in visual cortical slices prepared from age-matched dark-reared animals. Four days of binocular deprivation in otherwise normally reared animals had no effect on basal uptake, but significantly lowered NMDA-evoked Ca uptake at agonist concentrations greater than 25 microM. These data suggest that even brief manipulations of sensory experience are sufficient to alter visual cortical calcium regulation.
authors.affiliation: Center for Neural Science, Brown University, Providence, RI 02912.
authors.forename: D
authors.initials: D
authors.lastname: Feldman
authors.forename: J E
authors.initials: JE
authors.lastname: Sherin
authors.forename: W A
authors.initials: WA
authors.lastname: Press
authors.forename: M F
authors.initials: MF
authors.lastname: Bear
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D002118:Calcium / Q000493:pharmacokinetics*; D002415:Cats; D003624:Darkness; D007839:Functional Laterality / Q000502:physiology; D066298:In Vitro Techniques; D016202:N-Methylaspartate; D011188:Potassium / Q000494:pharmacology; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000502:physiology*; D012683:Sensory Deprivation; D014793:Visual Cortex / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology
chemical_list: D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter; D001224:Aspartic Acid; D016202:N-Methylaspartate; D011188:Potassium; D002118:Calcium