title: Protein phosphatase 1 activity in Drosophila mutants with abnormalities in mitosis and chromosome condensation.
abstract: A Drosophila gene encoding a protein phosphatase 1 (PP1) has been sequenced, and lethal mutations in this locus (87B) analysed. Two mutants (ck19e211 and ck19hs46), which disrupt mitosis, lack the 87B isoenzyme and express only approximately 20% of wild type PP1 activity. The promoter region of the gene is deleted in the ck19e211 mutant. A third mutant (ck19e078), which shows suppression of position effect variegation, but has little effect on mitosis, possesses approximately 35% of wild type PP1 activity. The results indicate that the PP1 87B isoenzyme is involved in regulation of chromosome condensation at interphase as well as mitosis.
authors.affiliation: Department of Biochemistry, The University, Dundee, Scotland, UK.
authors.forename: V
authors.initials: V
authors.lastname: Dombrádi
authors.forename: J M
authors.initials: JM
authors.lastname: Axton
authors.forename: H M
authors.initials: HM
authors.lastname: Barker
authors.forename: P T
authors.initials: PT
authors.lastname: Cohen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle; D002875:Chromosomes / Q000648:ultrastructure; D003001:Cloning, Molecular; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D005796:Genes; D007814:Larva; D008938:Mitosis; D008969:Molecular Sequence Data; D009154:Mutation; D010749:Phosphoprotein Phosphatases / Q000235:genetics* / Q000378:metabolism; D054645:Protein Phosphatase 1
chemical_list: D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1