Receptors for insulin and IGF-I in bovine lens epithelium.
The presence of insulin receptors has not yet been established in lens epithelial cells. Our goal was to understand the involvement of insulin in lens pathology, especially diabetic cataractogenesis. As a first step in this process, we evaluated the presence of insulin receptors in bovine lens epithelium. Insulin bound to insulin receptor with a high affinity (KD 7.0 nM), although this binding of insulin to its receptor could be replaced by IGF-I (KD 41 nM). Similarly IGF-I binding to IGF-I receptor (KD 0.38 nM) could be inhibited by insulin (KD 53.7 nM). SDS-PAGE and subsequent autoradiography of affinity labeled insulin receptor show the presence of a 130 kDa band, indicating that the lens epithelium insulin receptor may be similar to insulin receptors isolated from other tissues.
Sinha
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AC
Department of Human Biological Chemistry & Genetics, University of Texas Medical Branch, Galveston 77550.
Stuart
C A
CA
Srivastava
S K
SK
D000345:Affinity Labels; D000818:Animals; D001345:Autoradiography; D002417:Cattle; D002462:Cell Membrane / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004848:Epithelium / Q000737:chemistry; D007908:Lens, Crystalline / Q000737:chemistry*; D011869:Radioligand Assay; D011972:Receptor, Insulin / Q000032:analysis*; D011956:Receptors, Cell Surface / Q000032:analysis*; D017451:Receptors, Somatomedin
D000345:Affinity Labels; D011956:Receptors, Cell Surface; D017451:Receptors, Somatomedin; D011972:Receptor, Insulin