Isolation of the ERG12 gene of Saccharomyces cerevisiae encoding mevalonate kinase.
The ERG12 gene of Saccharomyces cerevisiae has been cloned by complementation of an erg12-1 mutation affecting mevalonate kinase. From the 2.8-kb insert isolated, the functional gene has been localized on a DNA fragment of 2.1 kb. The mRNA is 1.45 kb long. Gene disruption shows that the ERG12 gene is essential in yeast, both for spore germination and vegetative growth.
Oulmouden
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A
Laboratoire de Biochimie et Génétique des Microorganismes, Université de Poitiers, France.
Karst
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F
D003001:Cloning, Molecular; D005800:Genes, Fungal / Q000502:physiology; D010770:Phosphotransferases / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor); D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C021796:mevalonate kinase