[Dynamics of synaptosomal membranes in experimental neurosis in animals using donor-acceptor pairs of luminophores].
Dynamic of synaptosomal membrane's structural parameters (fluidity, protein clusterization, thickness of lipid bilayer) during chronic (15 days) psychogenic stress was compared with kinetic of membrane bound enzyme--Na,K-ATPase. For evaluation of structural changes in membranes a special multiprobe method, based on the use of fluorescent probes ANSA and pyrene, fluorescence of endogenous tryptophane and inductive-resonance energy transfer was designed. The data obtained indicates correlation between structural and functional changes in synaptosomal membranes. It was also concluded that the multiprobe procedure used is a sensitive and adequate tool for investigation of structural changes in biomembranes.
Abdulov
N A
NA
Eremenko
A V
AV
Val'dman
A V
AV
D000817:Anilino Naphthalenesulfonates; D000818:Animals; D001921:Brain / Q000378:metabolism; D005456:Fluorescent Dyes; D008297:Male; D011721:Pyrenes; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013315:Stress, Psychological / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism*; D013997:Time Factors; D014364:Tryptophan / Q000378:metabolism
D000817:Anilino Naphthalenesulfonates; D005456:Fluorescent Dyes; D011721:Pyrenes; C027132:1-anilino-8-naphthalenesulfonate; D014364:Tryptophan; D000254:Sodium-Potassium-Exchanging ATPase
Izuchenie dinamiki sinaptosomal'nykh membran pri éksperimental'noĭ nevrotizatsii zhivotnykh s ispol'zovaniem donorno--aktseptornykh par liuminoforov.