Repair of single nucleotide DNA mismatches transfected into mammalian cells can occur by short-patch excision.
Synthetic DNA linkers containing a single mismatched nucleotide (C:A) are repaired without bias at high efficiency when introduced into mammalian cells on a SV40 shuttle vector. From the pattern of repair in vectors containing multiple linkers, it appears that DNA synthesis following mismatch excision can replace a length of DNA as short as 40 nucleotides. Furthermore, results from the introduction of linker molecules containing combinations of single-strand nicks suggest that transient unsealed nicks do not drive the direction of mismatch repair in mammalian cells, as has previously been proposed.
Heywood
L A
LA
Biochemistry Laboratory, School of Biological Sciences, University of Sussex, Falmer, Brighton, Great Britain.
Burke
J F
JF
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002460:Cell Line; D004260:DNA Repair; D004261:DNA Replication; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000138:chemical synthesis; D010957:Plasmids; D015183:Restriction Mapping; D013539:Simian virus 40 / Q000235:genetics; D014162:Transfection
D015345:Oligonucleotide Probes