Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis.
The nucleotide sequence of the Japanese type of hepatitis C virus (HCV-J) genome, consisting of 9413 nucleotides, was determined by analyses of cDNA clones from plasma specimens from Japanese patients with chronic hepatitis. HCV-J genome contains a long open reading frame that can encode a sequence of 3010 amino acid residues. Comparison of HCV-J with the American isolate of HCV showed 22.6% difference in nucleotide sequence and 15.1% difference in amino acid sequence. Thus HCV-J and the American isolate of HCV are probably different subtypes of HCV. The relationship of HCV-J with other animal RNA virus families and the putative organization of the HCV-J genome are discussed.
Kato
N
N
Virology Division, National Cancer Center Research Institute, Tokyo, Japan.
Hijikata
M
M
Ootsuyama
Y
Y
Nakagawa
M
M
Ohkoshi
S
S
Sugimura
T
T
Shimotohno
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005814:Genes, Viral; D016174:Hepacivirus / Q000235:genetics* / Q000302:isolation & purification; D006526:Hepatitis C / Q000382:microbiology*; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid
D012367:RNA, Viral