title: Regulation of progesterone receptor-mediated transcription by phosphorylation.
abstract: The progesterone receptor (PR) in the chicken oviduct is a phosphoprotein that regulates gene transcription in the presence of progesterone. Treatment with progesterone in vivo stimulates phosphorylation of the progesterone receptor. With transient transfection assays, the present work has tested whether phosphorylation participates in the regulation of PR-mediated transcription. Treatment with 8-bromo-cyclic adenosine monophosphate (8-Br cAMP), a stimulator of cAMP-dependent protein kinase [protein kinase A (PKA)], mimicked progesterone-dependent, receptor-mediated transcription in the absence of progesterone. Inhibition of PKA blocked hormone action. Treatment with okadaic acid, an inhibitor of protein phosphatases 1 and 2A, stimulated transcription in a manner similar to that of progesterone. These observations suggest that phosphorylation of the PR or other proteins in the transcription complex can modulate PR-mediated transcription in vivo.
authors.affiliation: Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030.
authors.forename: L A
authors.initials: LA
authors.lastname: Denner
authors.forename: N L
authors.initials: NL
authors.lastname: Weigel
authors.forename: B L
authors.initials: BL
authors.lastname: Maxwell
authors.forename: W T
authors.initials: WT
authors.lastname: Schrader
authors.forename: B W
authors.initials: BW
authors.lastname: O'Malley
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D002645:Chickens; D005260:Female; D005786:Gene Expression Regulation; D007700:Kinetics; D010057:Oviducts / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors; D010766:Phosphorylation; D011374:Progesterone / Q000494:pharmacology*; D011980:Receptors, Progesterone / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection
chemical_list: D011980:Receptors, Progesterone; D015124:8-Bromo Cyclic Adenosine Monophosphate; D011374:Progesterone; D010749:Phosphoprotein Phosphatases