Molecular characterization of a functional cDNA encoding the rat substance P receptor.
Substance P is a member of the tachykinin peptide family and participates in the regulation of diverse biological processes. The polymerase chain reaction and conventional library screening were used to isolate a complementary DNA (cDNA) encoding the rat substance P receptor from brain and submandibular gland. By homology analysis, this receptor belongs to the G protein-coupled receptor superfamily. The receptor cDNA was expressed in a mammalian cell line and the ligand binding properties of the encoded receptor were pharmacologically defined by Scatchard analysis and tachykinin peptide displacement as those of a substance P receptor. The distribution of the messenger RNA for this receptor is highest in urinary bladder, submandibular gland, striatum, and spinal cord, which is consistent with the known distribution of substance P receptor binding sites. Thus, this receptor appears to mediate the primary actions of substance P in various brain regions and peripheral tissues.
Hershey
A D
AD
Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, MO 63110.
Krause
J E
JE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D019204:GTP-Binding Proteins / Q000378:metabolism; D015870:Gene Expression; D007421:Intestine, Small / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013363:Submandibular Gland / Q000032:analysis; D014018:Tissue Distribution; D001743:Urinary Bladder / Q000032:analysis
D012333:RNA, Messenger; D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter; D004247:DNA; D019204:GTP-Binding Proteins