Two structurally and kinetically distinct forms of Wolinella succinogenes nitrite reductase.
It is shown that the oxidized form of the hexa-haem nitrite reductase of Wolinella succinogenes exists in two structurally and functionally distinct forms, termed 'resting' and 'redox-cycled'. The nitrite reductase as initially isolated, termed 'resting', has five low-spin ferrihaem groups and one high-spin ferrihaem group. The reduction of these haem groups by Na2S2O4 occurs in two kinetically and spectrally distinct phases. In the slower phase the haem groups are reduced by dithionite with a limiting rate of 4 s-1. If the enzyme is re-oxidized after reduction with dithionite or with methyl viologen, the resulting ferric form, termed 'redox-cycled', possesses only low-spin haem centres and a rate of reduction in the slower phase that is no longer limited. In the resting form of the enzyme the high-spin ferrihaem group is weakly exchange-coupled to a low-spin haem group. It is proposed that in the redox-cycled form the exchange coupling occurs between two low-spin ferric haem groups. This change in spin state allows a more rapid rate of electron transfer to the coupled pair.
Blackmore
R S
RS
School of Biological Sciences, University of East Anglia, Norwich, U.K.
Brittain
T
T
Gadsby
P M
PM
Greenwood
C
C
Thomson
A J
AJ
D001438:Bacteroidaceae / Q000201:enzymology*; D002942:Circular Dichroism; D004227:Dithionite; D004578:Electron Spin Resonance Spectroscopy; D006418:Heme / Q000032:analysis / Q000378:metabolism; D007700:Kinetics; D009572:Nitrite Reductases / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D010269:Paraquat; D013053:Spectrophotometry
D004227:Dithionite; D006418:Heme; D009572:Nitrite Reductases; D010269:Paraquat