title: NMR and fluorometric characterization of mithramycin in aqueous solution.
abstract: The assignments for the resonances in the 1H and 13C NMR spectra of mithramycin in aqueous solution have been determined by a combination of 2D NMR methods. Specific NOESY contacts observed between different moieties indicate that the drug assumes a compact conformation in aqueous solution. Fluorescence measurements are consistent with a compact structure for mithramycin in water and confirm the preference of mithramycin for binding to dG-containing nucleic acids. These studies provide a basis for the further physico-chemical characterization of mithramycin-DNA complexes.
authors.affiliation: Comprehensive Cancer Center, University of Alabama, Birmingham 35294.
authors.forename: N R
authors.initials: NR
authors.lastname: Krishna
authors.forename: D M
authors.initials: DM
authors.lastname: Miller
authors.forename: T T
authors.initials: TT
authors.lastname: Sakai
mesh_terms: D004247:DNA / Q000378:metabolism; D005470:Fluorometry; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D015394:Molecular Structure; D008926:Plicamycin / Q000737:chemistry* / Q000378:metabolism; D011522:Protons; D012996:Solutions; D013050:Spectrometry, Fluorescence; D014867:Water
chemical_list: D011522:Protons; D012996:Solutions; D014867:Water; D004247:DNA; D008926:Plicamycin