Dibutyrylcytidine 3',5'-cyclic monophosphate stimulates neurite outgrowth in rat pheochromocytoma PC12.
Dibutyrylcytidine 3',5'-cyclic monophosphate (Bt2cCMP) stimulated neurite outgrowth in rat pheochromocytoma PC12 cells in a dose-dependent manner at the dose range from 0.5 mM to 6 mM. About 25% of the cells had neurites in response to 6 mM Bt2cCMP. In contrast with the effect of nerve growth factor (NGF) which increased the percentage of cells with neurites gradually during a 6-day culture period, the stimulating effect of Bt2cCMP reached a plateau 2 days after plating. Staurosporine inhibited the neurite outgrowth induced by NGF, but not that by Bt2cCMP. These results suggest that Bt2cCMP stimulates neuronal differentiation by a mechanism different from that by NGF.
Yanagida
M
M
Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Gohda
E
E
Yamamoto
I
I
D000310:Adrenal Gland Neoplasms; D000818:Animals; D001369:Axons / Q000187:drug effects / Q000502:physiology*; D003563:Cyclic CMP / Q000031:analogs & derivatives / Q000494:pharmacology*; D003597:Cytosine Nucleotides / Q000494:pharmacology*; D009414:Nerve Growth Factors / Q000494:pharmacology; D010673:Pheochromocytoma; D051381:Rats
D003597:Cytosine Nucleotides; D009414:Nerve Growth Factors; D003563:Cyclic CMP; C053441:dibutyryl cyclic-3',5'-cytidine monophosphate