Rapid detection of cytomegalovirus DNA in urine samples with a dot blot hybridization immunoenzymatic assay.
A dot blot hybridization immunoenzymatic assay for the rapid detection of cytomegalovirus DNA in urine samples was developed by using a digoxigenin-labeled probe which was immunoenzymatically visualized by antidigoxigenin Fab fragments labeled with alkaline phosphatase. A total of 516 urine samples from different groups of subjects were analyzed, and the hybridization assay was able to yield results within 24 h. The results obtained were compared with results for detection of cytomegalovirus antigens in infected cell cultures.
Musiani
M
M
Institute of Microbiology, University of Bologna, Italy.
Zerbini
M
M
Gentilomi
G
G
Gallinella
G
G
Venturoli
S
S
Gibellini
D
D
La Placa
M
M
D000956:Antigens, Viral / Q000302:isolation & purification; D003587:Cytomegalovirus / Q000276:immunology / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000382:microbiology / Q000652:urine; D015342:DNA Probes; D004279:DNA, Viral / Q000652:urine*; D005069:Evaluation Studies as Topic; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D007124:Immunoenzyme Techniques; D009693:Nucleic Acid Hybridization
D000956:Antigens, Viral; D015342:DNA Probes; D004279:DNA, Viral