Changes in cerebral inositol-1-phosphate concentrations in LiCl-treated rats: regional and strain differences.
LiCl-induced (5 mEq/kg) regional differences in the cerebral phosphoinositide (PI) cycle were studied by measuring inositol-1-phosphate (Ins-1-P), an intermediate in the PI cycle, in male Sprague Dawley and Han/Wistar rats by gas chromatography/mass spectrometry. Control Ins-1-P levels were higher frontally than caudally in both rat strains. LiCl increased Ins-1-P levels 1.8 to 7.4 fold in different regions of brain of Sprague Dawley rats but only 1.2 to 1.8 fold in Han/Wistar rats. This strain difference offers a way to compare the effects of lithium on PI metabolism versus receptor-G protein-phospholipase C coupling mechanisms.
Savolainen
K M
KM
Department of Pharmacology, University of Kansas Medical Center, Kansas City 66103.
Hirvonen
M R
MR
Tarhanen
J
J
Nelson
S R
SR
Samson
F E
FE
Pazdernik
T L
TL
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002421:Caudate Nucleus / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D002712:Chlorides / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D006624:Hippocampus / Q000378:metabolism; D007295:Inositol Phosphates / Q000378:metabolism*; D008094:Lithium / Q000494:pharmacology*; D018021:Lithium Chloride; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D014018:Tissue Distribution
D002712:Chlorides; D007295:Inositol Phosphates; C002647:inositol 1-phosphate; D008094:Lithium; D018021:Lithium Chloride