Effects of DNA-binding drugs on T4 DNA ligase.
A number of DNA intercalating and externally binding drugs have been found to inhibit nick sealing, cohesive and blunt end ligation, AMP-dependent DNA topoisomerization and EDTA-induced DNA nicking mediated by bacteriophage T4 DNA ligase. The inhibition seems to arise from drug-substrate interaction so that formation of active DNA-Mg2(+)-AMP-enzyme complex is impaired while assembled and active complexes are not disturbed by drug binding to the substrate.
Montecucco
A
A
Istituto di Genetica Biochimica ed Evoluzionistica, CNR, Pavia, Italy.
Pedrali-Noy
G
G
Spadari
S
S
Lestingi
M
M
Ciarrocchi
G
G
D000249:Adenosine Monophosphate / Q000378:metabolism; D011088:DNA Ligases / Q000037:antagonists & inhibitors*; D004264:DNA Topoisomerases, Type I / Q000378:metabolism; D004317:Doxorubicin / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D004996:Ethidium / Q000494:pharmacology; D015255:Idarubicin / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology; D007364:Intercalating Agents / Q000494:pharmacology*; D011118:Polynucleotide Ligases / Q000037:antagonists & inhibitors*; D013379:Substrate Specificity; D013604:T-Phages / Q000201:enzymology
D007211:Indoles; D007364:Intercalating Agents; D000249:Adenosine Monophosphate; C007293:DAPI; D004317:Doxorubicin; D004492:Edetic Acid; D004264:DNA Topoisomerases, Type I; D011088:DNA Ligases; D011118:Polynucleotide Ligases; D004996:Ethidium; D015255:Idarubicin