Inhibition of the in vitro generation of class II-restricted, HSV-1-specific, CD4+ CTL by HIV-1.
Previously, we demonstrated that cultures of human peripheral blood lymphocytes (PBL) stimulated with herpes simplex virus type 1 (HSV-1) generate antigen-specific, major histocompatibility complex (MHC) restricted cytotoxic T lymphocytes (CTL) that tend to be CD4+ and restricted to HLA-DR antigens. In this study, we present evidence that when HSV-1 stimulated human peripheral blood lymphocytes (PBL) are cocultured with human immunodeficiency virus type 1 (HIV-1), the generation of CD4+, DR-restricted CTL during the 5-day culture period is inhibited. In contrast, HIV-1 had no effect on either natural killer (NK) activity, or on the unrestricted NK-like killers which are often detected in HSV-1-stimulated cultures after the depletion of CD16+ cells. HIV-1 also failed to inhibit the generation of CTL against Epstein-Barr virus (EBV), a response that principally involves CD8+, CD4-, class I-restricted killers.
Mawle
A C
AC
Immunology Branch, Centers for Disease Control, U.S. Public Health Service, Atlanta, GA.
Thieme
M L
ML
Ridgeway
M R
MR
McDougal
J S
JS
Schmid
D S
DS
D000818:Animals; D002470:Cell Survival; D002472:Cell Transformation, Viral; D015497:HIV-1 / Q000276:immunology*; D006684:HLA-DR Antigens / Q000276:immunology*; D004854:Herpesvirus 4, Human; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D018139:Simplexvirus / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II