title: Identification of a cis-acting element in human immunodeficiency virus type 2 (HIV-2) that is responsive to the HIV-1 rev and human T-cell leukemia virus types I and II rex proteins.
abstract: A simian virus 40 late replacement vector encoding human immunodeficiency virus type 1 (HIV-1) gp120 (pGP120) was used to define a region within the HIV-2 genome that could work as a rev-responsive element (RRE). Our previous work showed that gp120 expression in this system required a functional RRE in cis and required the rev protein in trans (M.-L. Hammarskjöld, J. Heimer, B. Hammarskjöld, I. Sangwan, L. Albert, and D. Rekosh, J. Virol. 63:1959-1966, 1989). Using pGP120, we first mapped an RRE to a 1,042-base-pair (bp) Sau3a fragment in the env region of HIV-2. Both HIV-1 rev (rev1) and HIV-2 rev (rev2) could work in conjunction with this fragment. Further mapping showed that a 272-bp subfragment within the 1,042-bp region was sufficient as an RRE. Surprisingly, the smaller fragment worked only with the rev1 protein and not with its homologous rev2 protein. In addition, the rev2 protein failed to function together with the RRE from HIV-1. We also utilized this system to examine the ability of the rex genes of human T-cell leukemia virus types I and II to functionally substitute for rev. These experiments showed that complementation by both the rexI and rexII proteins required the presence of an RRE. The rex proteins worked well in conjunction with either the HIV-1 or the HIV-2 RRE (the 1,042-bp as well as the 272-bp fragment).
authors.affiliation: Department of Microbiology, State University of New York, Buffalo 14214.
authors.forename: N
authors.initials: N
authors.lastname: Lewis
authors.forename: J
authors.initials: J
authors.lastname: Williams
authors.forename: D
authors.initials: D
authors.lastname: Rekosh
authors.forename: M L
authors.initials: ML
authors.lastname: Hammarskjöld
mesh_terms: D001483:Base Sequence; D017977:Deltaretrovirus / Q000235:genetics*; D015694:Gene Products, rev / Q000235:genetics*; D005816:Genetic Complementation Test; D006678:HIV / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics; D015498:HIV-2 / Q000235:genetics; D015368:Human T-lymphotropic virus 1 / Q000235:genetics; D015367:Human T-lymphotropic virus 2 / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012045:Regulatory Sequences, Nucleic Acid; D015534:Trans-Activators / Q000235:genetics*; D054321:rev Gene Products, Human Immunodeficiency Virus
chemical_list: D015694:Gene Products, rev; D015534:Trans-Activators; D054321:rev Gene Products, Human Immunodeficiency Virus