Casein kinase II activities related to hyperphosphorylation of human papillomavirus type 16-E7 oncoprotein in epidermal keratinocytes.
The present study describes hyper-phosphorylation of E7-oncoprotein of human papillomavirus (HPV) type 16 in epidermal keratinocytes. We found that highly phosphorylated E7-oncoprotein was present in epidermal keratinocytes but little in fibroblasts. The E7 oncoprotein contains serine residues (Ser-Ser-Glu-Glu-Glu) capable of being phosphorylated by casein kinase II (CK II). Extracts from various cell lines including human origins transformed by HPV 16 were examined for the casein kinase activity. The results showed that CK II activity was present at significantly high levels in keratinocytes but little or no detectable levels of the activity in human fibroblasts. These differential CK II activities in host cells may play a part in the differential transforming activity by E7-oncoprotein.
Hashida
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Laboratory of Molecular and Cell Biology, Kanagawa Cancer Center Research Institute, Yokohama, Japan.
Yasumoto
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S
D000595:Amino Acid Sequence; D000818:Animals; D047388:Casein Kinases; D002478:Cells, Cultured; D004279:DNA, Viral / Q000235:genetics; D004817:Epidermis / Q000201:enzymology; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D015603:Keratinocytes / Q000201:enzymology*; D051379:Mice; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism*; D027383:Papillomaviridae / Q000235:genetics / Q000378:metabolism*; D050725:Papillomavirus E7 Proteins; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D015183:Restriction Mapping; D014162:Transfection
D004279:DNA, Viral; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; C059731:oncogene protein E7, Human papillomavirus type 16; D011494:Protein Kinases; D047388:Casein Kinases