Growth hormone stimulates the formation of sn-1,2-diacylglycerol in rat hepatocytes.
The transmembrane signaling events of GH were investigated in the liver, a major target organ of GH action. Recombinant human GH when added to freshly isolated rat hepatocytes rapidly stimulated the production of sn-1,2-diacylglycerol (DAG). The generation of DAG was biphasic with the first transient peak observed at 2 min and the second peak at 15 min (1.2-fold and 1.4-fold over control, respectively). Levels of DAG continued to be elevated above those in control cells at 30 min. The response was dose-dependent with an EC50 of 0.15 nM. Both bovine GH and rat GH, which bind to the rat GH receptor but not to the PRL receptor, also stimulated DAG production. Similarly, human PRL, which binds to the PRL but not GH receptor, stimulated DAG formation to a comparable extent. These results suggest that production of DAG may be an early signaling event mediated by hormone stimulation of both the GH and PRL receptors.
Johnson
R M
RM
Department of Developmental Biology, Genentech, Inc., South San Francisco, California 94080.
Napier
M A
MA
Cronin
M J
MJ
King
K L
KL
D000818:Animals; D019852:Diacylglycerol Kinase; D004075:Diglycerides / Q000378:metabolism*; D013006:Growth Hormone / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D009994:Osmolar Concentration; D010770:Phosphotransferases / Q000037:antagonists & inhibitors; D011744:Pyrimidinones / Q000494:pharmacology; D051381:Rats; D013844:Thiazoles / Q000494:pharmacology; D013997:Time Factors
C011439:1,2-diacylglycerol; D004075:Diglycerides; D011744:Pyrimidinones; D013844:Thiazoles; D013006:Growth Hormone; C047505:R 59022; D010770:Phosphotransferases; D019852:Diacylglycerol Kinase