Identification of a long stretch of homopurine.homopyrimidine sequence in a cluster of retroposons in the human genome.
A cluster of nine retroposons of four different types in a 6221 base EcoRI DNA fragment was isolated from a human fetal liver genomic library using a human nucleophosmin (B23) cDNA as a probe. These retroposons are: (1) a solitary HERV-K long terminal repeat upstream from; (2) a nucleophosmin processed pseudogene; (3) six Alu repeated sequences interspersed in both directions; and (4) a truncated Kpn repeated sequence integrated by an Alu monomer and the HERV-K long terminal repeat. Sequence analysis shows that the nucleophosmin pseudogene contains a long stretch (135 base-pairs) of homopurine.homopyrimidine (Pur.Pyr) sequence. S1 and P1 nuclease digestion indicated that this sequence was able to adopt a non-B-DNA triplex structure under either acidic or neutral conditions. This finding is the first example of the association of a potential DNA triplex structure with a cluster of retroposons.
Liu
Q R
QR
Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030.
Chan
P K
PK
D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004251:DNA Transposable Elements; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D000090243:Nucleophosmin; D011544:Pseudogenes; D011685:Purine Nucleotides; D011742:Pyrimidine Nucleotides; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D004251:DNA Transposable Elements; C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D011685:Purine Nucleotides; D011742:Pyrimidine Nucleotides; D000090243:Nucleophosmin; D004247:DNA