Evaluation of a new enzyme immunoassay (TESTPACK ROTAVIRUS) for diagnosis of viral gastroenteritis.
TESTPACK ROTAVIRUS, a simple 10 min enzyme immunoassay, was compared with reversed passive hemagglutination assay (RPHA) and polyacrylamide gel electrophoresis of virus RNA (RNA-PAGE) for the detection of rotaviral antigens in feces from 104 diarrheal children. The results of TESTPACK ROTAVIRUS were identical with those of RNA-PAGE. Discordant results of RPHA were further examined by latex agglutination and electron microscopy and were finally decided to be negative. Pararotaviruses, adenoviruses and small round viruses were negative in the TESTPACK ROTAVIRUS.
Honma
H
H
Department of Enteroviruses, National Institute of Health, Tokyo, Japan.
Ushijimma
H
H
Takagi
M
M
Kitamiura
T
T
D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D005759:Gastroenteritis / Q000175:diagnosis*; D006387:Hemagglutination, Viral; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards*; D011933:Reagent Kits, Diagnostic; D012401:Rotavirus; D012400:Rotavirus Infections / Q000175:diagnosis*
D011933:Reagent Kits, Diagnostic