Growth factor-induced release of a glycosyl-phosphatidylinositol (GPI)-linked protein from the HEp-2 human carcinoma cell line.
The release of a GPI-linked oncofetal protein (PLAP) from HEp-2 cells has been studied as an indicator of endogenous PI-specific PLC activation. A non-hydrolysable GTP analogue, EGF and insulin all produced a dose-dependent release of PLAP from these cells. This indicates that an increase in PLC activity following either growth factor binding or G protein activation may cause cellular release of PLAP.
Roberts
J M
JM
Department of Immunology, University of Liverpool, UK.
Kenton
P
P
Johnson
P M
PM
D000469:Alkaline Phosphatase / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D004798:Enzymes / Q000096:biosynthesis; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D066246:ErbB Receptors; D019204:GTP-Binding Proteins / Q000494:pharmacology; D006017:Glycolipids / Q000378:metabolism*; D017261:Glycosylphosphatidylinositols; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D007527:Isoenzymes / Q000378:metabolism*; D007822:Laryngeal Neoplasms / Q000201:enzymology; D010716:Phosphatidylinositols / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000201:enzymology
D004798:Enzymes; D006017:Glycolipids; D017261:Glycosylphosphatidylinositols; D007328:Insulin; D007527:Isoenzymes; D010716:Phosphatidylinositols; C055791:germ-cell AP isoenzyme; D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D011505:Protein-Tyrosine Kinases; D000469:Alkaline Phosphatase; D019204:GTP-Binding Proteins