Characterization of fos-induced osteogenic tumours and tumour-derived murine cell lines.
Osteogenic tumours from c-fos (MT-c-fos-LTR)-transgenic mice and from mice infected with the v-fos-bearing FBR murine osteosarcoma virus (FBR MSV) showed close morphological and neoplastic similarities. Fos mRNA expression was elevated in both types of tumours, and expression of several genes characteristic of differentiated bone cells was either lower, enhanced, or not detectable in comparison to that in normal bone. Tumour-derived cell lines showed variable levels of exogenous fos expression; bone-cell-specific genes were similarly expressed in both primary tumours and tumour-derived cell lines. Upon transplantation the tumour cells formed fibrosarcomas, some of which contained areas of focal osseochondrous differentiation. Non-tumorigenic cell lines established from bone tissue of normal and MT-c-fos-LTR transgenic mice showed osteoblastic characteristics, whereas no parathyroid hormone (PTH) response was observed in transgenic tumour cell lines in spite of high alkaline phosphatase activity. These data indicate that deregulated fos expression interferes with terminal osteogenic differentiation in v-fos- and c-fos-induced bone tumours.
Goralczyk
R
R
GSF-Abt. für Molekulare Zellpathologie, Neuherberg, Federal Republic of Germany.
Closs
E I
EI
Rüther
U
U
Wagner
E F
EF
Strauss
P G
PG
Erfle
V
V
Schmidt
J
J
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001846:Bone Development / Q000187:drug effects; D001859:Bone Neoplasms / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D000242:Cyclic AMP / Q000378:metabolism; D015870:Gene Expression; D051379:Mice; D008822:Mice, Transgenic; D009857:Oncogenes / Q000235:genetics; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism; D012516:Osteosarcoma / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D010281:Parathyroid Hormone / Q000494:pharmacology; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000494:pharmacology*; D016760:Proto-Oncogene Proteins c-fos
D010281:Parathyroid Hormone; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D000242:Cyclic AMP; D000469:Alkaline Phosphatase