Physicochemical characterization of corticotrophin releasing factor receptor in rat pituitary and brain.
The physicochemical characteristics of solubilized crosslinked CRF receptor-ligand complexes were studied in the anterior and intermediate pituitary lobes and brain of the rat. In all tissues studied, there was a major labeled band with a molecular weight of 72 +2- 3.5 kDa, (n = 15), 71 +/- 1.3 (n = 6), 73 +2- 2.5 (n = 7) and 75 +2- 3.5 (n = 7) kDa in the anterior and intermediate lobes of the pituitary, amygdala and cerebral cortex, respectively. The density of this band was inhibited by CRF analogs, but not by unrelated peptides. This is consistent with the comparable binding properties of CRF in membrane preparations of these tissues. These results suggest that differences in receptor regulation and the reported ability of CRF to stimulate cAMP is due to differences in ligand receptor processing and coupling to membrane transduction systems, rather than to major differences in the binding protein.
Flores
M
M
Section on Endocrine Physiology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.
Carvallo
P
P
Aguilera
G
G
D000679:Amygdala / Q000737:chemistry; D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D002540:Cerebral Cortex / Q000737:chemistry; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003346:Corticotropin-Releasing Hormone / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D008970:Molecular Weight; D010902:Pituitary Gland / Q000737:chemistry*; D010903:Pituitary Gland, Anterior / Q000737:chemistry; D051381:Rats; D011919:Rats, Inbred Strains; D018019:Receptors, Corticotropin-Releasing Hormone; D017981:Receptors, Neurotransmitter / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism
D003432:Cross-Linking Reagents; D018019:Receptors, Corticotropin-Releasing Hormone; D017981:Receptors, Neurotransmitter; C061959:corticotropin-releasing hormone, iodo-Tyr(0)-; D003346:Corticotropin-Releasing Hormone