[Formation of free radicals and DNA breaks after pulsed laser irradiation of DNA complexes with intercalating dyes].
Destruction of lambda phage DNA is studied under nanosecond pulse laser irradiation (lambda = 355 nm) of DNA-dye complexes in solution at 77K (dye--acridine orange, 8-methoxypsoralen, ethidium bromide). Free radicals induced by laser radiation are found to participate in DNA sugar-phosphate chain scission. It was observed that the quantity of DNA double-strand breaks correlated with that of the free radicals and that of oxygen influenced DNA laser destruction.
Zhizhina
G P
GP
Moskalenko
S A
SA
Siniak
V A
VA
Shekun
Iu G
IuG
Iakshin
M A
MA
D000165:Acridine Orange / Q000378:metabolism / Q000494:pharmacology; D010582:Bacteriophage lambda / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D004396:Coloring Agents / Q000378:metabolism / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D004249:DNA Damage; D004279:DNA, Viral / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D004578:Electron Spin Resonance Spectroscopy; D004996:Ethidium / Q000378:metabolism / Q000494:pharmacology; D005609:Free Radicals; D007364:Intercalating Agents / Q000378:metabolism / Q000494:pharmacology*; D007834:Lasers; D008730:Methoxsalen / Q000378:metabolism / Q000494:pharmacology; D010777:Photochemistry
D004396:Coloring Agents; D004279:DNA, Viral; D005609:Free Radicals; D007364:Intercalating Agents; D004247:DNA; D004996:Ethidium; D000165:Acridine Orange; D008730:Methoxsalen
Obrazovanie svobodnykh radikalov i razryvov DNK pri deĭstvii impul'snogo lazernogo izlucheniia na kompleksy DNK s krasiteliami-interkaliatorami.