Measurement of rotavirus-neutralizing coproantibody in children by fluorescent focus reduction assay.
A fluorescent focus reduction assay suitable for the measurement of rotavirus-neutralizing antibodies in the feces of children was developed. Of 408 stools tested, 7% showed false-positive neutralization, and the number of rotavirus serotypes neutralized by a fecal extract was proportional to the levels of antirotaviral immunoglobulin A in the extract.
Coulson
B S
BS
Department of Gastroenterology, Royal Children's Hospital, Parkville, Victoria, Australia.
Masendycz
P J
PJ
D000914:Antibodies, Viral / Q000032:analysis*; D002648:Child; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D005759:Gastroenteritis / Q000276:immunology; D006801:Humans; D009500:Neutralization Tests; D012401:Rotavirus / Q000276:immunology*; D012400:Rotavirus Infections / Q000276:immunology
D000914:Antibodies, Viral