The DNA polymerase from the archaebacterium Sulfolobus acidocaldarius: a thermophilic and thermoresistant enzyme which can perform automated polymerase chain reaction.
A DNA polymerase purified from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius was used to perform automated DNA amplification at 70 degrees C as well as site directed mutagenesis by Polymerase Chain Reaction (P.C.R.). The yield of amplification performed at optimum MgCl2 concentration for the Taq or the S. acidocaldarius DNA polymerase, for the same DNA target, was equivalent. The ability of S. acidocaldarius DNA polymerase to perform P.C.R. under less stringent requirement of MgCl2 concentration gives this enzyme a non-negligible advantage over the Taq DNA polymerase.
Salhi
S
S
Laboratoire de Biologie Moléculaire de la Réplication, UPR 272-CNRS, BP n8, Villejuif, France.
Elie
C
C
Jean-Jean
O
O
Meunier-Rotival
M
M
Forterre
P
P
Rossignol
J M
JM
de Recondo
A M
AM
D000260:Adenoviruses, Human / Q000235:genetics; D001483:Base Sequence; D041841:Bradyrhizobiaceae / Q000201:enzymology*; D004247:DNA / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000302:isolation & purification / Q000378:metabolism*; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005814:Genes, Viral; D006515:Hepatitis B virus / Q000235:genetics; D006358:Hot Temperature; D007256:Information Systems; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*; D015183:Restriction Mapping
D004247:DNA; D004259:DNA-Directed DNA Polymerase