[Cultivation of hepatitis A virus in various cell lines and isolation of DNA sequences].
Using the MBB/11/5 strain originally adapted to the PLC/PRF/5 cell line, reproduction of the virus in diploid cells of human embryo fibroblasts, continuous primate lines (RAMT, FRhK-4) and human urinary bladder tumor (T-24) lines was studied. We obtained HAV DNA sequences (about 99% from vRNA) for the MBB/11/5 strain which were used as a probe for demonstration of vRNA synthesis in the infected cells.
Farashian
V R
VR
Bychenko
A B
AB
Kuzaeva
I A
IA
Artemov
E K
EK
Khizhniakova
T M
TM
Zhdanov
V M
VM
D000818:Animals; D002460:Cell Line; D004247:DNA / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D006507:Hepatovirus / Q000235:genetics / Q000254:growth & development*; D006801:Humans; D009693:Nucleic Acid Hybridization; D009710:Nucleotide Mapping; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping; D014776:Virus Cultivation / Q000379:methods
D004279:DNA, Viral; D012367:RNA, Viral; D004247:DNA
Kul'tivirovanie virusa gepatita A v razlichnykh kletochnykh liniiakh i poluchenie DNK-posledovatel'nosteĭ.