A blocking ELISA to differentiate hog cholera virus antibodies in pig sera from those due to other pestiviruses.
The blocking ELISA technique was extended to comparative serology by using 3 different pestivirus strains: Hog cholera virus (HCV) Alfort strain propagated in PK15 cell line, Border disease virus (BDV) Aveyron strain in PK15 and BVD NADL** strain in fetal calf kidney (FCK) primary cells. Rabbit antisera to the Alfort HCV strain and Aveyron BDV strain were raised for use in the test. A bovine hyperimmune serum to BVD virus was also used for detecting antibodies specific to BVD virus. The ELISA was compared with the neutralisation test on various groups of field and experimetnal porcine sera. The results obtained with the ELISA were well correlated with the neutralisation test. Therefore the ELISA may be recommended as a differential serological test between HCV and other pestivirus antibodies in pig sera.
Leforban
Y
Y
Ministère de l'Agriculture, Centre national d'Etudes Vétérinaires et Alimentaires, Ploufragan, France.
Edwards
S
S
Ibata
G
G
Vannier
P
P
D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification*; D000956:Antigens, Viral / Q000276:immunology; D001667:Binding, Competitive; D006692:Classical Swine Fever Virus / Q000276:immunology*; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D009500:Neutralization Tests; D010576:Pestivirus / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral