Immunofluorescence and immunoperoxidase assays for the titration of infectious hepatitis A virus (HAV).
Immunofluorescence (IFA) and immunoperoxidase (IPA) assays were developed for the titration of infectious hepatitis A virus. Both of these methods were found to be simple, rapid and quantitatively reproducible. The immunoperoxidase technique could be the method of choice for the assay of HAV in cell culture.
Nadala
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Department of Microbiology, University of Hawaii, Honolulu 96822.
Loh
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D000818:Animals; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D005455:Fluorescent Antibody Technique; D006506:Hepatitis A / Q000175:diagnosis* / Q000473:pathology; D006507:Hepatovirus / Q000302:isolation & purification*; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000382:microbiology