Inhibition of histone H2B gene transcription and of cellular growth by a truncated viral trans-activator protein.
The herpes simplex virus virion protein Vmw65 trans activates the viral immediate-early genes and some octamer-containing cellular genes, including that encoding histone H2B. We found, however, that a truncated form of this virion protein repressed H2B gene transcription and also dominantly inhibited induction of the gene by intact Vmw65. A cell line expressing this truncated protein expressed reduced levels of H2B and grew more slowly than the parental cell line or a similar line expressing the intact protein.
Dent
C L
CL
Department of Biochemistry, University College and Middlesex School of Medicine, London, United Kingdom.
Estridge
J K
JK
Kemp
L M
LM
Latchman
D S
DS
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D006657:Histones / Q000235:genetics*; D007700:Kinetics; D007739:L Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D010957:Plasmids; D018139:Simplexvirus / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014162:Transfection; D014771:Virion / Q000235:genetics
D006657:Histones; D015534:Trans-Activators