Fibroblast growth factor receptor is a portal of cellular entry for herpes simplex virus type 1.
Herpes simplex virus type 1 (HSV-1) is a ubiquitous pathogen responsible for considerable morbidity in the general population. The results presented herein establish the basic fibroblast growth factor (FGF) receptor as a means of entry of HSV-1 into vertebrate cells. Inhibitors of basic FGF binding to its receptor and competitive polypeptide antagonists of basic FGF prevented HSV-1 uptake. Chinese hamster ovary (CHO) cells that do not express FGF receptors are resistant to HSV-1 entry; however, HSV-1 uptake is dramatically increased in CHO cells transfected with a complementary DNA encoding a basic FGF receptor. The distribution of this integral membrane protein in vivo may explain the tissue and cell tropism of HSV-1.
Kaner
R J
RJ
Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
Baird
A
A
Mansukhani
A
A
Basilico
C
C
Summers
B D
BD
Florkiewicz
R Z
RZ
Hajjar
D P
DP
D000327:Adsorption; D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D002462:Cell Membrane / Q000382:microbiology; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D005346:Fibroblast Growth Factors / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology; D006497:Heparan Sulfate / Q000378:metabolism; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000235:genetics / Q000502:physiology*; D017468:Receptors, Fibroblast Growth Factor; D018139:Simplexvirus / Q000502:physiology*; D014162:Transfection
D004247:DNA; D005346:Fibroblast Growth Factors; D006497:Heparan Sulfate; D010446:Peptide Fragments; D011956:Receptors, Cell Surface; D017468:Receptors, Fibroblast Growth Factor