title: Enhanced platelet reactivity in cats experimentally infected with feline infectious peritonitis virus.
abstract: Platelet function was evaluated in six specific-pathogen-free cats prior to and following intraperitoneal inoculation with feline infectious peritonitis virus (FIPV). By 4 days post-inoculation, platelet samples from five of six cats responded with irreversible platelet aggregation to threshold concentrations of adenosine diphosphate (ADP). This was accompanied by enhanced platelet 14C-serotonin release (greater than 10%) in two cats. Compared to one of six baseline samples, five of five post-inoculation samples exhibited microaggregate formation in response to 20 microM epinephrine. Enhanced platelet 14C-serotonin release did not accompany these responses. Enhanced platelet responses to ADP and epinephrine were also observed on day 11 post-inoculation and day 16 (when one cat died) or 21 (the end of the study). Platelet 14C-serotonin release in response to 20 microM epinephrine increased markedly in three of five cats on day 21. Enhanced collagen-induced platelet responses were not demonstrated. Although the mechanism for the enhanced platelet responses observed on day 4 was unknown, a direct effect on the virus on platelets, mononuclear inflammatory cells, and endothelial cells must be considered.
authors.affiliation: Auburn University, College of Veterinary Medicine, Department of Pathobiology, AL.
authors.forename: M K
authors.initials: MK
authors.lastname: Boudreaux
authors.forename: R C
authors.initials: RC
authors.lastname: Weiss
authors.forename: M
authors.initials: M
authors.lastname: Toivio-Kinnucan
authors.forename: N
authors.initials: N
authors.lastname: Cox
authors.forename: J S
authors.initials: JS
authors.lastname: Spano
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D002371:Cat Diseases / Q000097:blood* / Q000473:pathology; D002415:Cats; D003094:Collagen / Q000494:pharmacology; D003333:Coronaviridae Infections / Q000097:blood / Q000473:pathology / Q000662:veterinary*; D004837:Epinephrine / Q000494:pharmacology; D010538:Peritonitis / Q000097:blood / Q000473:pathology / Q000662:veterinary*; D010974:Platelet Aggregation / Q000187:drug effects; D012701:Serotonin / Q000097:blood; D013047:Specific Pathogen-Free Organisms
chemical_list: D012701:Serotonin; D000244:Adenosine Diphosphate; D003094:Collagen; D004837:Epinephrine