title: Immunohistochemical demonstration of fibre type-specific isozymes of cytochrome c oxidase in human skeletal muscle.
abstract: The immunohistochemical reaction of monoclonal as well as polyclonal antibodies against cytochrome c oxidase (COX) subunits with serial sections of normal human skeletal muscle was investigated. The stronger reactivity of polyclonal antibodies to COX subunits II-III and VIIbc with type I as compared to type II fibres, correlated well with the higher histochemical reactivity of NADH dehydrogenase, succinate dehydrogenase and cytochrome c oxidase in type I fibres. In contrast an almost exclusive reaction of a monoclonal antibody against subunit IV with type I fibre and a preponderant reaction of a polyclonal antibody against subunits Vab with type II fibres was obtained. Antibodies against subunits I, Vb and VIc did not reveal a fibre-type-specific reactivity. The data indicate in human muscle the occurrence of fibre type-specific isozymes of cytochrome c oxidase differing in subunits IV and Va or Vb.
authors.affiliation: Unite de Recherche Biologie et Pathologie Neuromusculaires INSERM U 153, CNRS UA 614, Paris, France.
authors.forename: N
authors.initials: N
authors.lastname: Romero
authors.forename: C
authors.initials: C
authors.lastname: Marsac
authors.forename: M
authors.initials: M
authors.lastname: Fardeau
authors.forename: M
authors.initials: M
authors.lastname: Droste
authors.forename: B
authors.initials: B
authors.lastname: Schneyder
authors.forename: B
authors.initials: B
authors.lastname: Kadenbach
mesh_terms: D000911:Antibodies, Monoclonal; D003576:Electron Transport Complex IV / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism*; D046911:Macromolecular Substances; D009132:Muscles / Q000166:cytology / Q000201:enzymology*
chemical_list: D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D046911:Macromolecular Substances; D003576:Electron Transport Complex IV