title: Subcellular biodistribution of 99Tcm(V) DMSA in squamous carcinoma: a comparative study in humans and in an animal tumour model.
abstract: Technetium-99m(99Tcm) (V) dimercaptosuccinic acid is a new imaging agent which has been used to evaluate head and neck squamous cell carcinoma (SCC). This study compared the subcellular biodistribution of 99Tcm(V)DMSA in an established rabbit tumour SCC model and in humans with head and neck SCC. In rabbits, approximately 17-37% of radioactivity was located on tumour cell membrane. Approximately 57-80% of radioactivity was located nonspecifically in tumour cytosol, only 2-6% was bound specifically to tumour mitochondria, and 1-4% bound specifically to microsomes. In humans, 25-45% of radioactivity was localized on tumour cell membrane and 28-60% localized nonspecifically in tumour cytosol. There was 11-20% of radioactivity specifically bound inside the cell to the mitochondria and 1-6% specifically bound to microsomes. These results show that although 99Tcm(V)DMSA is accumulated at sites of SCC, the localization process is nonspecific.
authors.affiliation: Department of Otolaryngology, Guy's Hospital, London.
authors.forename: J C
authors.initials: JC
authors.lastname: Watkinson
authors.forename: S
authors.initials: S
authors.lastname: Allen
authors.forename: M
authors.initials: M
authors.lastname: Higgins
authors.forename: A
authors.initials: A
authors.lastname: Bharij
authors.forename: C R
authors.initials: CR
authors.lastname: Lazarus
authors.forename: M N
authors.initials: MN
authors.lastname: Maisey
authors.forename: S E
authors.initials: SE
authors.lastname: Clarke
mesh_terms: D000368:Aged; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D005260:Female; D006258:Head and Neck Neoplasms / Q000378:metabolism*; D006801:Humans; D008297:Male; D008861:Microsomes / Q000378:metabolism; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism; D015609:Organotechnetium Compounds / Q000493:pharmacokinetics*; D011817:Rabbits; D004113:Succimer / Q000493:pharmacokinetics*; D019783:Technetium Tc 99m Dimercaptosuccinic Acid
chemical_list: D015609:Organotechnetium Compounds; D019783:Technetium Tc 99m Dimercaptosuccinic Acid; D004113:Succimer