title: Myeloperoxidase in human lung lavage. II. Internalization of myeloperoxidase by alveolar macrophages.
abstract: Bronchial wash and bronchoalveolar lavage were performed in 12 healthy subjects (five smokers), in order to elucidate whether or not material of neutrophil origin may be phagocytized by lung macrophages in vivo. Cells from different levels in the bronchial tree were obtained by sequential injection and subsequent aspiration of either four 50-ml or five 10-ml aliquots. Each aliquot was used for the determination of total and differential cell counts. The proportion of myeloperoxidase-positive alveolar macrophages was determined by specific immune histochemical staining. The percentage of myeloperoxidase-positive macrophages was highest (median 94.8%, range 37-98.5%) in the 10-ml aliquots and lowest in the last three 50-ml aliquots (median values 1-2.5%) (P less than 0.001). A significant correlation was obtained between the fraction of myeloperoxidase-positive macrophages and the percentage count of bronchoalveolar lavage neutrophils (r = 0.466, P less than 0.05). Furthermore, the cellular myeloperoxidase showed a significant inverse correlation (r = -0.46, P less than 0.05) to the viability of the bronchoalveolar lavage cells. Our findings are compatible with previous demonstrations in animals of neutrophil phagocytosis by lung macrophages and show that this phenomenon in particular occurs in the more proximal airways. The internalization of neutrophils or neutrophil components by airway macrophages may be an important scavenger mechanism for protection of the lung from the deleterious effects of activated neutrophils.
authors.affiliation: Department of Explorative Clinical Research, AB Draco, Lund, Sweden.
authors.forename: B
authors.initials: B
authors.lastname: Schmekel
authors.forename: Y
authors.initials: Y
authors.lastname: Hörnblad
authors.forename: M
authors.initials: M
authors.lastname: Linden
authors.forename: C
authors.initials: C
authors.lastname: Sundström
authors.forename: P
authors.initials: P
authors.lastname: Venge
mesh_terms: D000328:Adult; D000906:Antibodies; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D002452:Cell Count; D002470:Cell Survival; D005260:Female; D006801:Humans; D008264:Macrophages / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism; D009195:Peroxidase / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D010587:Phagocytosis / Q000502:physiology; D011650:Pulmonary Alveoli / Q000166:cytology; D012907:Smoking / Q000097:blood / Q000378:metabolism
chemical_list: D000906:Antibodies; D009195:Peroxidase