title: Regulatory elements of the raffinose operon: nucleotide sequences of operator and repressor genes.
abstract: The raffinose (raf) operon is negatively controlled by the specific binding of raf repressor (rafR gene) to raf operator (rafO) DNA. Both rafR and rafO have been sequenced. The 1,011-base-pair rafR gene encodes a 336-amino-acid polypeptide containing an N-terminal helix-turn-helix motif. rafO, as defined by in vivo titration of raf repressor, consists of two nearly identical 18-base-pair palindromes that flank the -35 box of the raf promoter.
authors.affiliation: Lehrstuhl für Genetik, Universität Regensburg, Federal Republic of Germany.
authors.forename: C
authors.initials: C
authors.lastname: Aslanidis
authors.forename: R
authors.initials: R
authors.lastname: Schmitt
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000378:metabolism*; D009876:Operon; D010957:Plasmids; D011887:Raffinose / Q000378:metabolism*; D015183:Restriction Mapping
chemical_list: D009844:Oligosaccharides; D011887:Raffinose