Simultaneous overexpression of avian pp60c-src and polyomavirus middle T antigen in mammalian cells.
Recombinant adenoviruses bearing the avian c-src gene and polyomavirus middle-T-antigen gene were isolated and used to simultaneously overexpress both proteins in human 293 cells. Cells overexpressing both proteins had greater middle-T-antigen-associated tyrosine kinase activity than cells overexpressing only middle T antigen. By contrast, the intrinsic pp60c-src tyrosine kinase activity was not greater in cells overexpressing both proteins than in cells overexpressing only pp60c-src. This system of simultaneous overexpression provides a means of obtaining large quantities of pp60c-src, middle T antigen, and the complex between them.
Cook
D N
DN
Institute for Molecular Biology and Biotechnology, McMaster University, Hamilton, Ontario, Canada.
Pavloff
N
N
Hassell
J A
JA
D000260:Adenoviruses, Human / Q000235:genetics; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D002460:Cell Line; D015870:Gene Expression; D005814:Genes, Viral; D006801:Humans; D007700:Kinetics; D015688:Oncogene Protein pp60(v-src) / Q000235:genetics* / Q000378:metabolism; D011120:Polyomavirus / Q000235:genetics* / Q000276:immunology; D011401:Promoter Regions, Genetic; D011505:Protein-Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism
D000952:Antigens, Polyomavirus Transforming; D011505:Protein-Tyrosine Kinases; D015688:Oncogene Protein pp60(v-src)