title: Transacting transcriptional activation of human spumaretrovirus LTR in infected cells.
abstract: The long terminal repeat (LTR) of the human spumaretrovirus (HSRV) was examined with respect to its ability to function as transcriptional promotor in virus-infected and uninfected cells. Transient transfections using a plasmid in which the 3' LTR of HSRV was coupled to the bacterial chloramphenicol acetyltransferase (cat) gene revealed that the level of HSRV LTR-directed cat gene expression was markedly increased in HSRV-infected cells compared to uninfected cells. Northern blot analysis of cat mRNA from transfected cultures suggests that transactivation of HSRV-directed gene expression occurs at the transcriptional level.
authors.affiliation: Institut für Virologie, Universität Würzburg, Federal Republic of Germany.
authors.forename: A
authors.initials: A
authors.lastname: Rethwilm
authors.forename: K
authors.initials: K
authors.lastname: Mori
authors.forename: B
authors.initials: B
authors.lastname: Maurer
authors.forename: V
authors.initials: V
authors.lastname: ter Meulen
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D005910:Glioma; D006801:Humans; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D012091:Repetitive Sequences, Nucleic Acid; D012190:Retroviridae / Q000235:genetics*; D016092:Spumavirus / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger; D015534:Trans-Activators; D015500:Chloramphenicol O-Acetyltransferase