title: Association of Plasmodium berghei proteins with the host erythrocyte membrane: binding to inside-out vesicles.
abstract: Two acidic phosphoproteins of Plasmodium berghei origin, of 65 and 46 kDa, are associated with the plasma membrane of the host mouse erythrocyte. The 65-kDa protein partitions between a soluble and particulate phase upon host cell lysis, whereas the 46-kDa protein is localized exclusively in the particulate fraction. Both proteins bind to inside-out vesicles derived from erythrocyte ghosts and the conditions of the reassociation reaction indicate that the binding is specific and that the proteins interact only with the cytoplasmic face of the erythrocyte membrane. The 65-kDa protein appears to exist in two membrane-associated states; one loosely bound, which readily dissociates from the membrane, and a more tightly associated state, which does not dissociate under non-denaturing conditions. The 46-kDa protein is tightly bound to the host erythrocyte membrane and does not dissociate. Cross-linking studies suggest that both of these parasite proteins interact with the submembrane cytoskeleton of the erythrocyte, and that the 65-kDa protein also appears to interact simultaneously with the lipid bilayer and erythrocyte membrane proteins. However, direct interaction between the malarial proteins and distinct erythrocyte membrane proteins could not be demonstrated. In summary, these findings indicate that the acidic phosphoproteins of the malarial parasite interact with the cytoplasmic face of the erythrocyte membrane both in vivo and in vitro.
authors.affiliation: MacArthur Center for Molecular Parasitology, Yale University School of Medicine, New Haven, CT.
authors.forename: M F
authors.initials: MF
authors.lastname: Wiser
authors.forename: A C
authors.initials: AC
authors.lastname: Sartorelli
authors.forename: C L
authors.initials: CL
authors.lastname: Patton
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001386:Azides; D003432:Cross-Linking Reagents; D003593:Cytoplasm; D004910:Erythrocyte Membrane / Q000378:metabolism*; D008565:Membrane Proteins / Q000097:blood; D051379:Mice; D010447:Peptide Hydrolases; D010750:Phosphoproteins / Q000378:metabolism; D010962:Plasmodium berghei / Q000502:physiology*; D011485:Protein Binding; D015800:Protozoan Proteins / Q000378:metabolism*; D013049:Spectrin / Q000378:metabolism
chemical_list: D000199:Actins; D001386:Azides; D003432:Cross-Linking Reagents; D008565:Membrane Proteins; D010750:Phosphoproteins; D015800:Protozoan Proteins; D013049:Spectrin; C025369:4,4'-dithiobis(phenylazide); D010447:Peptide Hydrolases