The effect of leukotriene B4, leukotriene C4, zymosan activated serum, histamine, tabanid extract and N-formyl-methionyl-leucyl-phenylalanine on the in vitro migration of equine eosinophils.
The migration of equine eosinophils under agarose in response to inflammatory mediators, an arthropod extract and a synthetic peptide was examined. A chemotactic index (CI) was calculated by determining the ratio of the distance of eosinophil migration towards the chemoattractant to the distance migrated towards a buffer. Differences between the CI of those eosinophils exposed to chemoattractants and those exposed only to buffer were assessed by an analysis of variance. All agents except leukotriene C4 and the buffer induced statistically significant directional migration of eosinophils. Leukotriene B4 (LTB4) was the most effective chemotaxin for equine eosinophils. Migration of eosinophils stimulated by 10(-9) M LTB4 exceeded that induced by concentrations of histamine six orders of magnitude greater. The response of equine eosinophils to inflammatory mediators was similar to the reported behavior of human eosinophils. The ability of tabanid extract to attract equine eosinophils suggests that arthropod induced tissue eosinophilia many not depend entirely upon immunological mechanisms. The peptide, N-formyl-methionyl-leucyl-phenylalanine attracted equine eosinophils at 10(-4) M and 10(-3) M, concentrations that exceed those reported to be stimulatory for eosinophils of other species. The results of this study indicate that equine eosinophils are capable of migrating towards diverse stimuli, of which LTB4 was the most effective. It is plausible that LTB4 figures prominently in equine inflammation, particularly in lesions dominated by eosinophils.
McEwen
B J
BJ
Department of Pathology, Ontario Veterinary College, University of Guelph.
Wilcock
B P
BP
Eyre
P
P
D000818:Animals; D002465:Cell Movement; D002478:Cells, Cultured; D002631:Chemotactic Factors, Eosinophil / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte; D004175:Diptera; D004804:Eosinophils / Q000276:immunology*; D006632:Histamine / Q000494:pharmacology; D006736:Horses; D007975:Leukotriene B4 / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D013189:SRS-A / Q000494:pharmacology; D014020:Tissue Extracts / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D002631:Chemotactic Factors, Eosinophil; D013189:SRS-A; D014020:Tissue Extracts; D007975:Leukotriene B4; D009240:N-Formylmethionine Leucyl-Phenylalanine; D006632:Histamine; D015054:Zymosan