Cell cycle control of DNA replication by a homologue from human cells of the p34cdc2 protein kinase.
The regulation of DNA replication during the eukaryotic cell cycle was studied in a system where cell free replication of simian virus 40 (SV40) DNA was used as a model for chromosome replication. A factor, RF-S, was partially purified from human S phase cells based on its ability to activate DNA replication in extracts from G1 cells. RF-S contained a human homologue of the Schizosaccharomyces pombe p34cdc2 kinase, and this kinase was necessary for RF-S activity. The limiting step in activation of the p34 kinase at the G1 to S transition may be its association with a cyclin since addition of cyclin A to a G1 extract was sufficient to start DNA replication. These observations suggest that the role of p34cdc2 in controlling the start of DNA synthesis has been conserved in evolution.
D'Urso
G
G
Department of Basic Sciences, Fred Hutchinson Cancer Center, Seattle, WA 98104.
Marraccino
R L
RL
Marshak
D R
DR
Roberts
J M
JM
D002051:Burkitt Lymphoma; D016203:CDC2 Protein Kinase / Q000235:genetics / Q000502:physiology*; D002453:Cell Cycle; D016213:Cyclins / Q000494:pharmacology; D004261:DNA Replication; D006801:Humans; D007399:Interphase; D010766:Phosphorylation; D012568:Schizosaccharomyces / Q000201:enzymology; D013539:Simian virus 40 / Q000235:genetics* / Q000502:physiology; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D016213:Cyclins; D016203:CDC2 Protein Kinase