Bile salt-induced increases in duodenal brush-border membrane proton permeability, fluidity, and fragility.
Rabbit duodenal brush-border membrane vesicles were treated in vitro with deoxycholate, glycodeoxycholate, or taurodeoxycholate. Intravesicular [14C]glucose space at equilibrium, 0.54 microliters/mg protein, was reduced by exposure to the three bile salts in a concentration (0.1-5.0 mM)-dependent manner, equatable with increased membrane fragility. Net proton permeability (Pnet), determined by acridine orange fluorescence quenching, was increased from 6.3 x 10(-4) cm/sec in untreated vesicles, by approximately 120, 150, and 170%, by treatment with bile salts at 0.1, 0.5 and 1.0 mM, respectively. The three bile salts were equipotent. The increases in membrane fragility and Pnet were not accompanied by significant increases in membrane fluidity, as assessed from steady-state and time-resolved diphenylhexatriene fluorescence anisotropy. The data demonstrate direct effects of bile salts on duodenal apical membrane fragility and proton permeability that are likely to be early events in bile salt-induced mucosal damage.
Zhao
D L
DL
Department of Physiological Sciences, University of Newcastle upon Tyne Medical School, England.
Hirst
B H
BH
D000818:Animals; D001647:Bile Acids and Salts / Q000493:pharmacokinetics* / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects*; D004386:Duodenum / Q000187:drug effects* / Q000648:ultrastructure; D005260:Female; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects*; D008871:Microvilli / Q000187:drug effects; D011522:Protons; D011817:Rabbits
D001647:Bile Acids and Salts; D011522:Protons