Polypeptide composition of rotavirus empty capsids and their possible use as a subunit vaccine.
Two types of empty capsid particles that differed with respect to the presence of the two outer shell proteins were isolated from MA-104 cells infected with bovine rotavirus V1005. Three previously uncharacterized polypeptides, I, II, and III, migrating between VP2 and VP6, were detected in empty capsids but not in single- and double-shelled rotavirus particles. Peptide mapping revealed that all three proteins were related to VP2. Polypeptides I, II, and III could be generated by in vitro trypsin digestion of empty capsids not exposed to trypsin in the infection medium. Labeled polypeptides appeared in empty capsids before they were detected in intracellular single- or double-shelled rotavirus particles. Empty capsids were also observed in MA-104 cells infected with bovine rotaviruses UK and NCDV, simian rotavirus SA11, and human rotavirus KU. VP7-containing empty capsid is the minimal subunit vaccine for cows; we failed to induce a substantial neutralizing antibody increase with VP7 purified under denaturating or nondenaturating conditions or with synthetic peptides corresponding to two regions of VP7.
Brüssow
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H
Nestlé Research Centre, NESTEC Ltd., Vers-chez-les-Blanc, Lausanne, Switzerland.
Bruttin
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A
Marc-Martin
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S
D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification*; D002213:Capsid / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification*; D002417:Cattle; D002460:Cell Line; D002501:Centrifugation, Zonal; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008253:Macaca mulatta; D046911:Macromolecular Substances; D008715:Methionine / Q000378:metabolism; D012401:Rotavirus / Q000032:analysis* / Q000276:immunology; D012697:Serine Endopeptidases; D014357:Trypsin; D014529:Uridine / Q000378:metabolism; D014765:Viral Vaccines
D000956:Antigens, Viral; D046911:Macromolecular Substances; D014765:Viral Vaccines; D008715:Methionine; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D014357:Trypsin; D014529:Uridine