title: Interactions of taurine with GABAB binding sites in mouse brain.
abstract: The interactions of taurine with GABAB receptors were studied in membranes from the brain of the mouse by measuring the binding of [3H]baclofen and that of [3H]GABA in the presence of isoguvacine. Taurine displaced ligand binding to GABAB receptors concentration-dependently with an IC50 in the micromolar range. The effects of baclofen on the release of taurine and GABA from slices of cerebral cortex of the mouse were assessed using a superfusion system. Potassium-stimulated release of both [3H]taurine and [3H]GABA was unaffected by baclofen but potentiated by delta-aminovalerate. The enhancement of release of [3H]taurine by delta-aminovalerate was partially antagonized by baclofen, suggesting that baclofen-sensitive receptors could modify the release.
authors.affiliation: Department of Biomedical Sciences, University of Tampere, Finland.
authors.forename: P
authors.initials: P
authors.lastname: Kontro
authors.forename: S S
authors.initials: SS
authors.lastname: Oja
mesh_terms: D000596:Amino Acids / Q000494:pharmacology; D021542:Amino Acids, Neutral; D000818:Animals; D001418:Baclofen / Q000494:pharmacology; D001923:Brain Chemistry / Q000187:drug effects*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D011188:Potassium / Q000494:pharmacology; D011963:Receptors, GABA-A / Q000187:drug effects*; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism; D013654:Taurine / Q000494:pharmacology*
chemical_list: D000596:Amino Acids; D021542:Amino Acids, Neutral; D011963:Receptors, GABA-A; D013654:Taurine; C013809:5-aminovaleric acid; D001418:Baclofen; D011188:Potassium