title: A pair of tandemly repeated genes code for gp24, a putative adhesion protein of Dictyostelium discoideum.
abstract: The glycoprotein gp24 has been implicated in cell-cell adhesion of Dictyostelium discoideum. We have used a cDNA clone that codes for gp24 to screen cloned genomic fragments. Two closely linked genes (GP24A and GP24B) were recognized that generate mRNAs of about 650 base pairs after excision of a small intron and addition of poly(A). They appear to have arisen by tandem duplication of about 800 base pairs, followed by divergence. These genes are expressed within a few hours of the initiation of development; their mRNAs accumulate to a peak at 12 hr and persist until culmination. Both genes have short guanine-rich sequences (G boxes) upstream that have been shown to be involved in transcriptional regulation of other genes expressed during development of Dictyostelium. Their mRNAs code for proteins that are 85% identical. GP24A and GP24B mRNAs code for proteins with a hydrophobic domain followed by a highly charged carboxyl-terminal domain.
authors.affiliation: Department of Biology, University of California-San Diego, La Jolla 92093.
authors.forename: W F
authors.initials: WF
authors.lastname: Loomis
authors.forename: D L
authors.initials: DL
authors.lastname: Fuller
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004271:DNA, Fungal / Q000235:genetics; D004023:Dictyostelium / Q000235:genetics* / Q000254:growth & development; D005656:Fungal Proteins / Q000235:genetics*; D015870:Gene Expression; D005800:Genes, Fungal; D007438:Introns; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D011487:Protein Conformation; D015183:Restriction Mapping
chemical_list: D004251:DNA Transposable Elements; D004271:DNA, Fungal; D005656:Fungal Proteins; D008562:Membrane Glycoproteins