Insulin binding and receptor tyrosine kinase activity in rat liver and skeletal muscle: effect of starvation.
Insulin binding and insulin receptor kinase activity were measured in solubilized and partially purified receptor preparations from liver and skeletal muscles of rats that were either fed a standard diet or subjected to a 72-hour fasting period. Insulin binding capacity was increased in both tissues from fasted rats as determined by Scatchard analysis. The affinity of the receptors was not modified by fasting. Affinity labeling of the alpha-subunit of insulin receptors also suggested an increase in the number of insulin receptors in both tissues. The ability of insulin to stimulate the autophosphorylation of the beta-subunit as well as the phosphorylation of the artificial substrate Glu80-Tyr20 was significantly impaired in liver from fasted rats and by contrast unchanged in skeletal muscles. These findings indicate that in rats, fasting produces changes in insulin receptor kinase activity in liver but not in muscle. The physiological significance of this tissue-specific regulation of receptor kinase activity in relation to insulin action during fasting remains to be established.
Balage
M
M
Laboratoire d'Etude du Métabolisme Azoté, Centre de Clermont-Theix, Ceyrat, France.
Grizard
J
J
Sornet
C
C
Simon
J
J
Dardevet
D
D
Manin
M
M
D000345:Affinity Labels / Q000378:metabolism; D000818:Animals; D001835:Body Weight; D007328:Insulin / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D009132:Muscles / Q000378:metabolism*; D009928:Organ Specificity; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011972:Receptor, Insulin / Q000302:isolation & purification / Q000378:metabolism*; D012016:Reference Values; D013217:Starvation; D013388:Succinimides / Q000378:metabolism
D000345:Affinity Labels; D007328:Insulin; D013388:Succinimides; D011505:Protein-Tyrosine Kinases; D011972:Receptor, Insulin; C019358:disuccinimidyl suberate