Regulation of 12(S)-hydroxyeicosatetraenoic acid (12(S)-HETE) binding sites on human epidermal cells by interferon-gamma.
We recently detected specific high-affinity binding sites for 12(S)-HETE, the main arachidonic acid metabolite in skin, on epidermal cells. The putative receptor is involved in keratinocyte chemotaxis toward 12(S)-HETE, which points to its participation in wound healing. In an effort to further characterize the 12(S)-HETE receptor, we investigated its regulation by various cytokines. Of the tested cytokines, only interferon (IFN)-gamma led to a massive induction of the 12(S)-HETE receptors. The effect was dose and time dependent and blocked by cycloheximide. The up-regulation of 12(S)-HETE receptors by IFN-gamma may represent an amplification mechanism of the assumed role of 12(S)-HETE in skin wound repair.
Süss
R
R
Department of Dermatology, University of Munich, Federal Republic of Germany.
Arenberger
P
P
Gross
E C
EC
Ruzicka
T
T
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D001665:Binding Sites / Q000187:drug effects; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism*; D007371:Interferon-gamma / Q000494:pharmacology; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D011956:Receptors, Cell Surface / Q000187:drug effects* / Q000378:metabolism; D018055:Receptors, Eicosanoid; D012878:Skin Neoplasms / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D013997:Time Factors; D015854:Up-Regulation / Q000187:drug effects*
C066652:12-hydroxyeicosatetraenoic acid receptor; D006893:Hydroxyeicosatetraenoic Acids; D011956:Receptors, Cell Surface; D018055:Receptors, Eicosanoid; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D007371:Interferon-gamma; D003513:Cycloheximide