title: Role of primary and secondary maternal viremia in transplacental guinea pig cytomegalovirus transfer.
abstract: The modulation of the outcome of intrauterine guinea pig cytomegalovirus (GPCMV) infection by maternal viremia was investigated in the guinea pig model. Virus assay and in situ hybridization were used to study GPCMV infection of maternal blood, placentas, and fetuses following inoculation of pregnant guinea pigs by the subcutaneous, intracardiac, or intranasal route. Animals were inoculated in early gestation and were evaluated every 7 to 10 days throughout pregnancy. Although placental and fetal infections occurred in all groups examined, transfer of GPCMV to placentas and fetuses was most efficient in mothers inoculated subcutaneously. Primary viremia was followed by virus clearance from blood and by an episode of secondary viremia in the three groups of mothers examined. Placental and fetal infections in animals infected subcutaneously or intracardially were first detected at the time of primary viremia, persisted throughout gestation, and increased during secondary viremia. In contrast, placental and fetal infections in animals inoculated intranasally were demonstrated primarily during secondary viremia. Fetal infection was detected in all mothers with detectable primary and secondary viremia but in only 33% of mothers that experienced only primary viremia. These results suggest that secondary maternal viremia is associated with increased placental and fetal GPCMV infections.
authors.affiliation: Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06510.
authors.forename: B P
authors.initials: BP
authors.lastname: Griffith
authors.forename: M
authors.initials: M
authors.lastname: Chen
authors.forename: H C
authors.initials: HC
authors.lastname: Isom
mesh_terms: D000022:Abortion, Spontaneous; D000818:Animals; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000635:transmission*; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005260:Female; D006168:Guinea Pigs; D008431:Maternal-Fetal Exchange; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious; D014766:Viremia / Q000635:transmission*
chemical_list: D015342:DNA Probes; D004279:DNA, Viral