title: Detection of human papillomavirus in skin and genital lesions of renal allograft recipients by in situ hybridization.
abstract: Renal allograft recipients have an increased incidence of malignancy including squamous carcinoma of cervix and skin. There is growing evidence that human papillomavirus (HPV) has a part to play in malignant transformation at these sites. We have previously identified HPV DNA in the skin and genital lesions of such patients by dot and Southern blotting. In situ hybridization studies, using biotinylated DNA probes for HPV 4, 5 and 8 in skin lesions and 6, 11, 16 and 18 in genital lesions, were performed on tissues derived from the same group of patients. In the cutaneous lesions, only 25% of the specimens probed were found to contain virus by in situ hybridization; 60% of these specimens were found to harbour virus by dot and Southern blotting. In situ hybridization revealed HPV 16 and/or 18 in 86% of the genital lesions probed.
authors.affiliation: Department of Pathology, Edinburgh University, Scotland.
authors.forename: K
authors.initials: K
authors.lastname: Blessing
authors.forename: K M
authors.initials: KM
authors.lastname: McLaren
authors.forename: R
authors.initials: R
authors.lastname: Morris
authors.forename: B B
authors.initials: BB
authors.lastname: Barr
authors.forename: E C
authors.initials: EC
authors.lastname: Benton
authors.forename: M
authors.initials: M
authors.lastname: Alloub
authors.forename: M H
authors.initials: MH
authors.lastname: Bunney
authors.forename: I W
authors.initials: IW
authors.lastname: Smith
authors.forename: G E
authors.initials: GE
authors.lastname: Smart
authors.forename: C C
authors.initials: CC
authors.lastname: Bird
mesh_terms: D000328:Adult; D000368:Aged; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D016030:Kidney Transplantation / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae; D012871:Skin Diseases / Q000382:microbiology* / Q000473:pathology; D014184:Transplantation, Homologous; D014412:Tumor Virus Infections / Q000235:genetics / Q000473:pathology*; D014591:Uterine Diseases / Q000382:microbiology* / Q000473:pathology
chemical_list: D004279:DNA, Viral