Concanavalin A affects beta-tubulin mRNA expression during neuritic processes of mouse neuroblastoma N18TG2 cells in a different manner from colchicine.
Addition of concanavalin A (Con A) to mouse neuroblastoma N18TG2 cells cultured with dibutyryl-cAMP which can stimulate neurite outgrowth, stopped the neuritic processes effectively. The extended neurites showed a gradual retraction for at least 8 hrs after addition of Con A, while addition of colchicine caused rapid retraction of the neurites. Immunocytochemistry showed that the addition of Con A did not disorganize the microtubules but the addition of colchicine did. The increase in beta-tubulin mRNA expression which was observed after cell culture and after stimulation by dB-cAMP was suppressed by the addition of Con A. Con A did not affect the beta-tubulin mRNA expression when the cells had already been cultured, while colchicine drastically decreased it. Thus, Con A appeared to affect the beta-tubulin mRNA expression in a different manner from colchicine, probably through inhibition of cell movement.
Katayama
N
N
Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Yamagata
Y
Y
Hashimoto
H
H
Kanazawa
H
H
Tsuchiya
T
T
Tsuda
M
M
D000818:Animals; D001369:Axons / Q000187:drug effects / Q000648:ultrastructure; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D003078:Colchicine / Q000494:pharmacology*; D003208:Concanavalin A / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects*; D007700:Kinetics; D051379:Mice; D009447:Neuroblastoma; D012333:RNA, Messenger / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects*; D014404:Tubulin / Q000235:genetics*; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000187:drug effects / Q000502:physiology
D012333:RNA, Messenger; D014404:Tubulin; D003208:Concanavalin A; D003994:Bucladesine; D003078:Colchicine