title: Expression of the erythropoietin receptor on a human myeloma cell line.
abstract: We demonstrated the expression of the erythropoietin (EPO) receptor by a human myeloma cell line (MM-S1) which was established in our laboratory. EPO dose-dependently stimulated the proliferation of MM-S1 cells. Binding of radioiodinated EPO (125I-Epo) to MM-S1 cells was competitively inhibited by unlabeled EPO, but not by other recombinant cytokines. Specific binding of 125I-Epo to MM-S1 cells was saturable, and the Scatchard analysis revealed 330 EPO binding sites per cell with a Kd of 0.56 nmol/L. Bound EPO was internalized by MM-S1 cells during incubation at 37 degrees C. This is the first report describing the expression of the EPO receptor by human cells other than those of the erythroid lineage.
authors.affiliation: Second Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Okuno
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: A
authors.initials: A
authors.lastname: Suzuki
authors.forename: S
authors.initials: S
authors.lastname: Ichiba
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: K
authors.initials: K
authors.lastname: Hitomi
authors.forename: R
authors.initials: R
authors.lastname: Sasaki
authors.forename: H
authors.initials: H
authors.lastname: Imura
mesh_terms: D002455:Cell Division / Q000187:drug effects; D004921:Erythropoietin / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D009101:Multiple Myeloma / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000378:metabolism; D017467:Receptors, Erythropoietin; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
chemical_list: D011956:Receptors, Cell Surface; D017467:Receptors, Erythropoietin; D004921:Erythropoietin