title: Serum antibody response to B. pertussis Tn5 mutants, purified PT and FHA in two different mouse strains and passive protection in the murine intranasal infection model.
abstract: The protective capacities of antibodies to pertussis toxin (PT) were compared with antibodies to several other pertussis antigens in an experimental murine model of intranasal infection with Bordetella pertussis. Protection from lethal challenge was achieved by passive immunization with mouse antisera to whole cells of the virulent B. pertussis BP338(Vir+) and its Tn5-generated mutants, BP353(Fha-), BP348(Adc-Hly-) and to a lesser extent of BP347(Vir-). The immune sera were produced in two different mouse strains, a good PT antibody responder (NIH) and a poor responder (F1 of CBA x C57BL/6). The antisera produced in the F1 mice contained no detectable neutralizing antibodies to PT as measured by the CHO cell assay. In spite of this the anti-BP353(Fha-) and BP348(Adc-Hly-) sera of the F1 mice seemed as protective as those of the NIH mice. A strong dependence between PT neutralizing antibody and protection was seen only when comparing sera of NIH and F1 mice immunized with purified active PT. The protective capacity of sera of both mouse strains immunized with purified filamentous hemagglutinin (FHA) correlated with their anti-FHA titers measured by enzyme immunoassay. The data thus confirm the protective capacity of anti-PT and anti-FHA, but also show that antibodies of other specificities can confer protection.
authors.affiliation: Department of Bacteriology, National Public Health Institute, Helsinki, Finland.
authors.forename: R M
authors.initials: RM
authors.lastname: Olander
authors.forename: A
authors.initials: A
authors.lastname: Muotiala
authors.forename: M
authors.initials: M
authors.lastname: Karvonen
authors.forename: T
authors.initials: T
authors.lastname: Kuronen
authors.forename: K
authors.initials: K
authors.lastname: Runeberg-Nyman
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D001885:Bordetella Infections / Q000517:prevention & control; D001886:Bordetella pertussis / Q000235:genetics / Q000276:immunology*; D004251:DNA Transposable Elements / Q000235:genetics; D005260:Female; D006388:Hemagglutinins / Q000276:immunology*; D007116:Immunization, Passive; D051379:Mice; D009154:Mutation; D009500:Neutralization Tests; D037342:Pertussis Toxin; D010567:Pertussis Vaccine / Q000276:immunology*; D010566:Virulence Factors, Bordetella / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D004251:DNA Transposable Elements; D006388:Hemagglutinins; D010567:Pertussis Vaccine; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin