title: Adverse effects of hyperbaric oxygen on [3H]uridine incorporation and uridine kinase activity in B104 rat neuroblastoma cells.
abstract: The effects of hyperbaric oxygen on uracil nucleotide metabolism in B104 rat neuroblastoma cells were investigated. Cells exposed to 10 atm O2 for 4 h incorporated markedly less [3H]uridine into the acid-soluble fraction and RNA compared to cells kept in ambient air. The acid-soluble fraction of the oxygen-treated cells contained less total [3H]uridine phosphates ([3H]UMP + [3H]UDP + [3H]UTP) than air-treated cells. Uridine kinase activity, assayed in cytosolic extracts from cells exposed to 10 atm O2 for 4 h, was decreased by 46% compared to the air controls. The reduced enzyme activity which appears to account for the depressed [3H]uridine incorporation, may contribute to the lethal effects of oxygen in these cells.
authors.affiliation: Department of Pharmacology, University of Pennsylvania, School of Medicine, Philadelphia 19104-6084.
authors.forename: G J
authors.initials: GJ
authors.lastname: Gendimenico
authors.forename: N
authors.initials: N
authors.lastname: Haugaard
mesh_terms: D000818:Animals; D009447:Neuroblastoma / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology*; D010770:Phosphotransferases / Q000378:metabolism*; D011312:Pressure; D051381:Rats; D014407:Tumor Cells, Cultured; D014529:Uridine / Q000378:metabolism*; D014541:Uridine Kinase / Q000378:metabolism*
chemical_list: D010770:Phosphotransferases; D014541:Uridine Kinase; D010100:Oxygen; D014529:Uridine