Establishment and characterization of a new Epstein-Barr virus transformed cell line from a human B cell lymphoma.
We have established a new cell line from a patient with centrocytic B cell lymphoma. Highly purified peripheral blood B cells from patient DUL (WBC counts 158,000/microliters) were infected in vitro with Epstein-Barr virus (EBV), and CD20+ B cells were cloned into 96 well culture plates with the aid of a cell sorter autoclone device. As shown by GTG-banding and Southern blot analysis, out-growing EBV-positive clones had the same chromosomal abnormalities and identical monoclonal IgH gene rearrangement as the original EBV-genome-negative leukemic B cell clone. Surface marker analysis with a panel of monoclonal antibodies revealed identical patterns on EBV-negative and -positive clones, with the exception of PCA1 (reactive with plasma cells) which was negative on freshly explanted leukemic B cells but positive on EBV-converted clones.
Janssen
O
O
Department of Medical Microbiology, University of Ulm, Federal Republic of Germany.
Heinze
B
B
Bartram
C R
CR
Pawlita
M
M
Binder
T
T
Heimpel
H
H
Diehl
K
K
Kabelitz
D
D
D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology; D002461:Cell Line, Transformed; D002467:Cell Nucleus / Q000473:pathology; D002472:Cell Transformation, Viral; D002869:Chromosome Aberrations; D003593:Cytoplasm / Q000473:pathology; D004279:DNA, Viral / Q000032:analysis; D006680:HLA Antigens / Q000032:analysis; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D007621:Karyotyping; D008223:Lymphoma / Q000235:genetics / Q000276:immunology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D010641:Phenotype
D000954:Antigens, Surface; D004279:DNA, Viral; D006680:HLA Antigens