Evidence for superoxide dismutase and catalase in mollicutes and release of reactive oxygen species.
The presence of superoxide dismutase was demonstrated in 21 strains of mollicutes, including achuloplasmas, mycoplasmas, and ureaplasmas. No superoxide dismutase activities or only traces were detectable in fresh prepared cell lysates, whereas activities were evident after dialysis of the cell lysates. A further increase in superoxide dismutase activities was observed after the cell lysates were heated to 65 degrees C for 30 s. This might be due to the destruction of enzymatic reactions interfering with the activity tests. Additionally, catalase activities were demonstrated in nearly 50% of the cell lysates, whereas no peroxidase activities were detectable. The production of O2- and H2O2 with glucose as substrate was demonstrated for 8 of 10 strains tested. No correlation to the pathogenicities of the strains was indicated. Anaerobic mycoplasmas showed the highest amount of radical production, whereas superoxide dismutase and catalase activities were in the range of activities estimated for aerobic mollicutes.
Meier
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Chemisches Institut, Tierärztlichen Hochschule, Hannover, Federal Republic of Germany.
Habermehl
G G
GG
D000127:Acholeplasma / Q000201:enzymology*; D001421:Bacteria, Anaerobic / Q000201:enzymology; D002374:Catalase / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D007700:Kinetics; D009174:Mycoplasma / Q000201:enzymology*; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism; D014509:Ureaplasma / Q000201:enzymology*
D013481:Superoxides; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase