title: In vitro properties of human melanoma cells metastatic in nude mice.
abstract: We have developed a human melanoma metastasis model in nude mice. In this model, a human variant cell line (451-LU) was obtained that spontaneously metastasized in nude mice. This variant cell line was selected from the lung of a nude mouse after several in vivo passages of human melanoma WM164 cells previously isolated from a melanoma metastasis of a patient. The WM164 cells were not competent for metastasis in nude mice prior to this selection. We compared the phenotypes of the parental nonmetastatic cell line and the metastatic variant with respect to growth at clonal seeding densities in protein-free medium (growth factor independence), in vitro invasion through reconstructed basement membranes, secretion of proteolytic enzymes, expression of tumor-associated antigens, and chromosomal abnormalities. Metastatic 451-LU cells showed significantly increased growth factor independence when grown at clonal seeding densities as compared to the parental cells. In in vitro chemoinvasion assays, metastatic 451-LU cells were significantly more invasive than the parental cells. The metastatic variant secreted collagenase and tissue type plasminogen activator at levels 10- and 3-fold higher than the parental WM164 cells, respectively. Polyclonal antibodies to tissue type plasminogen activator significantly inhibited invasion through reconstructed basement membranes. In metastatic 451-LU cells, expression of nerve growth factor receptor was elevated, both at the protein and transcriptional level. Metastatic cells were aneuploid with a mode of 97 chromosomes, whereas the parental nonmetastatic cells had a mode of 52 chromosomes. Our studies suggest that metastatic melanoma cell variants selected in vivo show increased independence of exogenous growth factors when grown at clonal cell densities, enhanced invasiveness in vitro, greater secretion of proteolytic enzymes, and increased chromosome mode as compared to the nonmetastatic parental cells. The data further suggest that melanoma cells isolated from metastatic lesions and maintained in vitro have an unstable invasive phenotype but that metastatic variant cells can readily be selected.
authors.affiliation: Wistar Institute of Anatomy and Biology, University of Pennsylvania, Philadelphia.
authors.forename: D
authors.initials: D
authors.lastname: Herlyn
authors.forename: D
authors.initials: D
authors.lastname: Iliopoulos
authors.forename: P J
authors.initials: PJ
authors.lastname: Jensen
authors.forename: A
authors.initials: A
authors.lastname: Parmiter
authors.forename: J
authors.initials: J
authors.lastname: Baird
authors.forename: H
authors.initials: H
authors.lastname: Hotta
authors.forename: K
authors.initials: K
authors.lastname: Adachi
authors.forename: A H
authors.initials: AH
authors.lastname: Ross
authors.forename: J
authors.initials: J
authors.lastname: Jambrosic
authors.forename: H
authors.initials: H
authors.lastname: Koprowski
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis; D002455:Cell Division; D002460:Cell Line; D002465:Cell Movement; D015342:DNA Probes; D005260:Female; D006801:Humans; D007621:Karyotyping; D008545:Melanoma / Q000473:pathology* / Q000503:physiopathology; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis / Q000473:pathology*; D009368:Neoplasm Transplantation; D009414:Nerve Growth Factors / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000032:analysis / Q000235:genetics; D017475:Receptors, Nerve Growth Factor; D014183:Transplantation, Heterologous
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D015342:DNA Probes; D009414:Nerve Growth Factors; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor