Transforming growth factor-beta 1 specifically localizes in elastin during synovial inflammation: an immunoelectron microscopic study.
We report here that extracellular TGF-beta 1 is associated exclusively with microfibrils of elastin which are present in the extracellular matrix of the inflamed articular joint of the rat. Inflammation was initiated by bacterial cell walls localized in the synovium following intraperitoneal injection of the bacterial components. This synovitis is associated with both destruction of connective tissue components and matrix deposition. The growth factor was localized by using a polyclonal antibody raised to a synthetic peptide corresponding to amino terminal 30 amino acids of TGF-beta 1 in conjunction with a gold-labeled secondary antibody. The results suggest a close association of TGF-beta 1 with proteoglycans which are known to be a major component of the microfibrils in elastin. Proteoglycan-mediated binding and concentration of TGF-beta 1 in specific areas of the extracellular matrix may constitute a mechanism whereby the growth factor could be targeted to specific sites of action.
Heine
U I
UI
Biological Carcinogenesis Development Program, Program Resources, Inc., National Cancer Institute-Frederick Cancer Research Facility, Maryland.
Wahl
S M
SM
Munoz
E F
EF
Allen
J B
JB
Ellingsworth
L R
LR
Flanders
K C
KC
Roberts
A B
AB
Sporn
M B
MB
D000818:Animals; D002473:Cell Wall / Q000276:immunology; D004549:Elastin / Q000378:metabolism*; D005260:Female; D008854:Microscopy, Electron; D051381:Rats; D011917:Rats, Inbred Lew; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018125:Receptors, Transforming Growth Factor beta; D013047:Specific Pathogen-Free Organisms; D013297:Streptococcus pyogenes / Q000166:cytology; D013585:Synovitis / Q000276:immunology / Q000378:metabolism*; D015291:Transforming Growth Factors / Q000378:metabolism*
D011956:Receptors, Cell Surface; D018125:Receptors, Transforming Growth Factor beta; D015291:Transforming Growth Factors; D004549:Elastin