Infrequent involvement of c-fos in avian leukosis virus-induced nephroblastoma.
To determine whether c-fos is involved in avian leukosis virus-induced nephroblastoma, 28 tumors from chickens were analyzed for novel fos fragments. DNA from 1 of 16 clonal outgrowths (in chicken 6561) contained novel fos-related EcoRI and KpnI fragments which hybridized to both v-fos and viral probes. Oncogenicity tests using filtered 6561 tumor cell homogenates did not reveal a tumor-inducing transduction of c-fos. We conclude that c-fos is only an occasional target for proviral insertions or new transductions in avian leukosis virus-induced nephroblastoma. The results also identify a polymorphism in c-fos in K28 chickens and demonstrate that unintegrated viral DNA is not a general characteristic of avian leukosis virus-induced nephroblastoma.
Collart
K L
KL
Department of Pathology, University of Massachusetts Medical Center, Worcester 01655.
Aurigemma
R
R
Smith
R E
RE
Kawai
S
S
Robinson
H L
HL
D000818:Animals; D001354:Avian Leukosis Virus; D015139:Blotting, Southern; D002645:Chickens; D015342:DNA Probes; D007680:Kidney Neoplasms / Q000235:genetics* / Q000382:microbiology; D009857:Oncogenes; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D016760:Proto-Oncogene Proteins c-fos; D015183:Restriction Mapping; D009396:Wilms Tumor / Q000235:genetics* / Q000382:microbiology
D015342:DNA Probes; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos