Inactivation of the thymidine kinase gene of a gI deletion mutant of pseudorabies virus generates a safe but still highly immunogenic vaccine strain.
In an earlier report, we described the construction of the genetically engineered pseudorabies virus strain 2.4N3A which does not express glycoprotein gI. Although this strain showed a strongly reduced virulence in 10-week-old seronegative pigs, it could still cause severe disease or death in 3-day-old piglets. To attenuate the strain further, we constructed mutants with a deletion in the viral thymidine kinase gene. One mutant strain, designated 783, has a deletion of 19 base pairs and was shown to be highly immunogenic and safe for vaccination of pigs against pseudorabies virus.
Moormann
R J
RJ
Department of Virology, Central Veterinary Institute (CDI), Lelystad, The Netherlands.
de Rover
T
T
Briaire
J
J
Peeters
B P
BP
Gielkens
A L
AL
van Oirschot
J T
JT
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002872:Chromosome Deletion; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005814:Genes, Viral; D011558:Herpesvirus 1, Suid / Q000201:enzymology / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D015183:Restriction Mapping; D013552:Swine; D013937:Thymidine Kinase / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*; D014765:Viral Vaccines
D004279:DNA, Viral; D015345:Oligonucleotide Probes; D015678:Viral Structural Proteins; D014765:Viral Vaccines; D013937:Thymidine Kinase