title: Wilms tumor locus on 11p13 defined by multiple CpG island-associated transcripts.
abstract: Wilms tumor is an embryonal kidney tumor involving complex pathology and genetics. The Wilms tumor locus on chromosome 11p13 is defined by the region of overlap of constitutional and tumor-associated deletions. Chromosome walking and yeast artificial chromosome (YAC) cloning were used to clone and map 850 kilobases of DNA. Nine CpG islands, constituting a "CpG island archipelago," were identified, including three islands that were not apparent by conventional pulsed-field mapping, and thus were at least partially methylated. Three distinct transcriptional units were found closely associated with a CpG island within the boundaries of a homozygous DNA deletion in a Wilms tumor.
authors.affiliation: Department of Genetics, Hospital for Sick Children, Toronto, Ontario, Canada.
authors.forename: L
authors.initials: L
authors.lastname: Bonetta
authors.forename: S E
authors.initials: SE
authors.lastname: Kuehn
authors.forename: A
authors.initials: A
authors.lastname: Huang
authors.forename: D J
authors.initials: DJ
authors.lastname: Law
authors.forename: L M
authors.initials: LM
authors.lastname: Kalikin
authors.forename: M
authors.initials: M
authors.lastname: Koi
authors.forename: A E
authors.initials: AE
authors.lastname: Reeve
authors.forename: B H
authors.initials: BH
authors.lastname: Brownstein
authors.forename: H
authors.initials: H
authors.lastname: Yeger
authors.forename: B R
authors.initials: BR
authors.lastname: Williams
mesh_terms: D002874:Chromosome Mapping; D016386:Chromosome Walking; D002880:Chromosomes, Human, Pair 11; D015342:DNA Probes; D015226:Dinucleoside Phosphates; D016162:Genes, Wilms Tumor / Q000235:genetics*; D006801:Humans; D014158:Transcription, Genetic; D009396:Wilms Tumor / Q000235:genetics*
chemical_list: D015342:DNA Probes; D015226:Dinucleoside Phosphates; C015772:CpG dinucleotide