A model system for studying the adsorption of a hydrophobic mutagen to dietary fibre.
The adsorption of 1,8-dinitropyrene (DNP) to alpha-cellulose has been studied as a model system for examining the adsorption of a hydrophobic mutagen to dietary fiber. Most of the DNP rapidly disappeared from an aqueous solution and partitioned between the glass wall of the test tube and the alpha-cellulose. Factors affecting DNP distribution included (i) the time of incubation, (ii) the final concentration of the solvent, dimethyl sulphoxide, in which the DNP has been dissolved, and (iii) the relative concentrations of DNP and alpha-cellulose. We suggest that this model system could be applied to other mutagens, and that alpha-cellulose would provide a useful standard fiber to permit inter-laboratory comparisons.
Roberton
A M
AM
Department of Biochemistry, University of Auckland, New Zealand.
Harris
P J
PJ
Hollands
H J
HJ
Ferguson
L R
LR
D000327:Adsorption; D002482:Cellulose / Q000494:pharmacology*; D004043:Dietary Fiber / Q000493:pharmacokinetics*; D004121:Dimethyl Sulfoxide / Q000633:toxicity; D009152:Mutagenicity Tests; D011721:Pyrenes / Q000493:pharmacokinetics* / Q000633:toxicity; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics*
D004043:Dietary Fiber; D011721:Pyrenes; C033310:1,8-dinitropyrene; D002482:Cellulose; D004121:Dimethyl Sulfoxide