Identification of a locus that regulates multiple functions in Pseudomonas solanacearum.
When Pseudomonas solanacearum K60 carries a multicopy plasmid containing cosmid clone pE6C (from the wild-type strain K60) or pBE6 (from the nonpathogenic strain B1), several phenotypic changes are observed, including the following: loss of virulence, reduced extracellular polysaccharide production, and increased polygalacturonase activity. Both cosmids contain a common 8-kilobase DNA region that is required for the phenotype shift. Saturation mutagenesis of pBE6 with Tn3-gus suggested that a single transcriptional unit of at least 1 kilobase is responsible for the phenotype shift. In maxicell assays, subclones containing this transcriptional unit expressed a single protein of about 25 kilodaltons.
Huang
Y
Y
Department of Plant Pathology, University of Wisconsin, Madison 53706.
Sequeira
L
L
D003360:Cosmids; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D005809:Genes, Regulator; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D011549:Pseudomonas / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D013752:Tetracycline / Q000494:pharmacology
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D013752:Tetracycline