title: Antigens of infectious laryngotracheitis herpesvirus defined by monoclonal antibodies.
abstract: Monoclonal antibodies to glycoprotein and protein antigens of infectious laryngotracheitis virus (ILTV) were divided into five groups on the basis of their reactivity in immunofluorescence and Western blotting. Group I antibodies recognised a single band of 60 K and Group II antibodies recognised bands of 205, 160, 115, 90 and 85 k in Western blotting. In immunofluorescence both these groups of antibodies reacted with antigens located in the cytoplasm of fixed virus-infected cells and they also reacted with unfixed cells, suggesting that these antigens are on the surface of virus-infected cells. While Group I monoclonal antibodies did not react with extracts of tunicamycin-treated cells, some Group II antibodies recognised bands of decreased molecular weight compared to those present in untreated cells. The reactivity of the Group II antibodies with extracts of tunicamycin-treated cells suggested that they recognised at least three different epitopes which was confirmed by ELISA additivity assays. Monoclonal antibodies of Group III, Group IV and Group V recognised several low molecular weight proteins from 45 to 24 k. Immunofluorescence studies showed that these were nuclear and cytoplasmic antigens that were not present on the surface of virus-infected cells.
authors.affiliation: CSIRO, Division of Animal Health, Parkville, Victoria, Australia.
authors.forename: J J
authors.initials: JJ
authors.lastname: York
authors.forename: S
authors.initials: S
authors.lastname: Sonza
authors.forename: M R
authors.initials: MR
authors.lastname: Brandon
authors.forename: K J
authors.initials: KJ
authors.lastname: Fahey
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000276:immunology; D001352:Herpesvirus 1, Gallid / Q000187:drug effects / Q000276:immunology*; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D014415:Tunicamycin / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D006023:Glycoproteins; D014415:Tunicamycin