Synergism between platelet-activating factor and diacylglycerol in the induction of superoxide anion production in guinea pig polymorphonuclear leukocytes.
The superoxide anion (O2-) production of guinea pig polymorphonuclear leukocytes (PMNL) by platelet-activating factor (PAF) was greatly enhanced by the addition of 1-oleoyl-2-acetylglycerol (OAG), even at low concentrations of OAG at which O2- production was little induced. The enhanced production was biphasic, depending on concentrations of PAF. One was saturable at a lower concentration range of PAF and probably mediated through a putative PAF receptor-islet-activating protein-sensitive GTP-binding protein (G protein) pathway. Another was increased in a concentration-dependent manner at a higher concentration range of PAF and apparently mediated through both receptor-G protein-dependent and -independent pathways. These results suggest that at least two types of action of PAF are involved in the synergistic stimulation of O2- production by PAF and OAG in PMNL.
Ohtsuka
T
T
Department of Physiological Chemistry, Hiroshima University School of Medicine, Japan.
Hiura
M
M
Ozawa
M
M
Okamura
N
N
Ishibashi
S
S
D000818:Animals; D004075:Diglycerides / Q000494:pharmacology*; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D005989:Glycerides / Q000494:pharmacology*; D006168:Guinea Pigs; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D010972:Platelet Activating Factor / Q000031:analogs & derivatives / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism*; D013844:Thiazoles; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D004075:Diglycerides; D005989:Glycerides; D010972:Platelet Activating Factor; D013844:Thiazoles; D010566:Virulence Factors, Bordetella; D013481:Superoxides; C038237:1-oleoyl-2-acetylglycerol; C048549:ONO 6240; D010100:Oxygen