Salt-dependent structure change and ion binding in cytochrome c studied by two-dimensional proton NMR.
To search for salt-dependent structure changes that might help to explain physicochemical differences observed in previous solution studies, two-dimensional proton NMR spectra of reduced and oxidized cytochrome c were recorded at relatively high and low salt concentrations. The results rule out substantial ionic strength dependent structure change in either redox form over the salt concentrations tested (5 mM phosphate to 5 mM phosphate plus 200 mM NaCl, at pH 7). Chemical shift changes were found for several residues within a limited segment of the oxidized protein, most prominently in the sequence Lys-86, Lys-87, Lys-88, Thr-89. A salt-dependent binding of phosphate anion(s) at this site, as observed earlier by others, is indicated. The binding of one or two phosphates at the cytochrome c surface can explain earlier small-angle X-ray scattering observations of an increase in the calculated radius of gyration of the oxidized protein at the same low-salt condition used here. Other observations, by ultraviolet resonance Raman and 1D NMR spectroscopies, of salt-dependent changes could not be corroborated, but may depend on the still lower salt used and the absence of phosphate. The results obtained support the view that the absence of sizeable redox-dependent structure change observed in X-ray and NMR studies at varying salt conditions is characteristic of the protein at all salt conditions above the low millimolar range. Physicochemical differences between oxidized and reduced cytochrome c apparently represent differences in stability without patent structure change.
Feng
Y
Y
Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia 19104-6059.
Englander
S W
SW
D000595:Amino Acid Sequence; D000818:Animals; D003574:Cytochrome c Group / Q000378:metabolism*; D006736:Horses; D007477:Ions; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D009994:Osmolar Concentration; D011485:Protein Binding; D011487:Protein Conformation; D012965:Sodium Chloride / Q000494:pharmacology; D014961:X-Ray Diffraction / Q000379:methods
D003574:Cytochrome c Group; D007477:Ions; D012965:Sodium Chloride