Relationship of chemotactic receptors for formyl peptide and C5a to CR1, CR3, and Fc receptors on human neutrophils.
The co-expression of C5a and formyl-methionine-leucine-phenylalanine-lysine (FMLPL) receptors with CR1, CR3, and Fc receptors on human neutrophils (PMN) was studied. Fluorescein-conjugated C5a (FL-C5a) and FMLPL (FL-FMLPL) were used to identify C5a and formyl peptide receptors. CR1, CR3, and Fc receptors were identified with monoclonal antibodies and a Texas red-labeled goat anti-mouse immunoglobulin second step reagent. The co-expression of chemotactic receptors with CR1, CR3, or Fc receptors was evaluated using two-color flow cytometry. A direct correlation between the degree of expression of receptors for FL-FMLPL and the expression of CR3, CR1, and Fc receptors on individual PMN was observed. In contrast, no correlation between the degree of C5a receptor expression and CR1, CR3, or Fc receptor expression was found. Similar results were obtained with PMN after up regulation of CR1, CR3, Fc, and FMLPL receptors by incubation at 37 degrees C for 10 min with or without phorbol myristate acetate. These data suggest that the expression of FMLPL, CR1, CR3, and Fc receptors are regulated in a similar manner, whereas C5a receptor expression is regulated independently. Furthermore, these data indicate that within a given population of PMN, a parallel exists between the degree of CR1, CR3, FMLPL, and Fc receptor expression on individual cells.
Van Epps
D E
DE
Applied Sciences, Baxter Healthcare Corporation, Round Lake, Illinois 60073.
Bender
J G
JG
Simpson
S J
SJ
Chenoweth
D E
DE
D000911:Antibodies, Monoclonal / Q000276:immunology; D003179:Complement C3b / Q000276:immunology / Q000378:metabolism; D015936:Complement C5a / Q000378:metabolism*; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006801:Humans; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000031:analogs & derivatives* / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism / Q000648:ultrastructure*; D011951:Receptors, Complement / Q000378:metabolism*; D017463:Receptors, Complement 3b; D011961:Receptors, Fc / Q000378:metabolism*; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D011961:Receptors, Fc; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic; D009240:N-Formylmethionine Leucyl-Phenylalanine; D003179:Complement C3b; D015936:Complement C5a; C049813:N-formylmethionyl-leucyl-phenylalanyl-lysine