Tetrahydrocannabinol inhibits adenyl cyclase in human leukemia cells.
Delta 9-tetrahydrocannabinol has been shown to induce incomplete maturation in ML2 human leukemia cell lines. We extend the observation of its induction of morphologic maturation to HL60 cells and of its induction of growth restriction to HL60 and K562 cells. We show that tetrahydrocannabinol reduces the cyclic AMP content of ML2 cells. Finally we demonstrate that this agent inhibits adenyl cyclase activity in ML2 cell membrane-enriched fractions. This finding in myeloid cells is compatible with one hypothesis of cannabinoid action in neuronal cells.
Rowley
J T
JT
Department of Medicine, University of Rochester School of Medicine, New York.
Rowley
P T
PT
D000067956:Adenylyl Cyclase Inhibitors; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D000242:Cyclic AMP / Q000378:metabolism; D013759:Dronabinol / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D007938:Leukemia; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*
D000067956:Adenylyl Cyclase Inhibitors; D013759:Dronabinol; D000242:Cyclic AMP