NMR and fluorometric characterization of mithramycin in aqueous solution.
The assignments for the resonances in the 1H and 13C NMR spectra of mithramycin in aqueous solution have been determined by a combination of 2D NMR methods. Specific NOESY contacts observed between different moieties indicate that the drug assumes a compact conformation in aqueous solution. Fluorescence measurements are consistent with a compact structure for mithramycin in water and confirm the preference of mithramycin for binding to dG-containing nucleic acids. These studies provide a basis for the further physico-chemical characterization of mithramycin-DNA complexes.
Krishna
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NR
Comprehensive Cancer Center, University of Alabama, Birmingham 35294.
Miller
D M
DM
Sakai
T T
TT
D004247:DNA / Q000378:metabolism; D005470:Fluorometry; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D015394:Molecular Structure; D008926:Plicamycin / Q000737:chemistry* / Q000378:metabolism; D011522:Protons; D012996:Solutions; D013050:Spectrometry, Fluorescence; D014867:Water
D011522:Protons; D012996:Solutions; D014867:Water; D004247:DNA; D008926:Plicamycin