Factors contributing to the decrease in concanavalin A-induced colony-stimulating factors in acute suppressive graft-versus-host disorder.
We have recently demonstrated that IL-3 and GM-CSF content in concanavalin A--stimulated splenic cultures was severely depressed for several weeks in mice undergoing an acute graft-vs.-host reaction (GVHR). Several factors contributed to this decrease in CSF levels at different points in the first 10 weeks of GVH. Myeloid lineage cells, both GM-CFU and Mac-1+ cells, increased in the spleen during GVH weeks 3-6, and (125I)-GM-CSF binding by spleen cells increased 10 fold--hence CSF content in supernatants was probably reduced by local consumption during these weeks. During GVH weeks 3-12, levels of all T cells--and of L3T4+ T cells, in particular--were reduced, limiting production of T cell derived lymphokines. Finally, during the first six weeks of GVH, immune suppression was found in cocultures of normal F1 and GVH spleens. Furthermore, during weeks 3-5, nonspecific suppression was found, affecting CSF production not only by normal host spleens but also by parental donor or unrelated spleen cells as well.
Hakim
F T
FT
Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
Pluznik
D H
DH
Shearer
G M
GM
D000818:Animals; D003115:Colony-Stimulating Factors / Q000378:metabolism*; D003208:Concanavalin A / Q000494:pharmacology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006087:Graft vs Host Reaction / Q000276:immunology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D007377:Interleukin-3 / Q000378:metabolism; D008264:Macrophages / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007376:Interleukin-2; D007377:Interleukin-3; D003208:Concanavalin A; D016178:Granulocyte-Macrophage Colony-Stimulating Factor