Tissue, developmental, and tumor-specific expression of divergent transcripts in Wilms tumor.
The Wilms tumor locus on chromosome 11p13 has been mapped to a region defined by overlapping, tumor-specific deletions. Complementary DNA clones representing transcripts of 2.5 (WIT-1) and 3.5 kb (WIT-2) mapping to this region were isolated from a kidney complementary DNA library. Expression of WIT-1 and WIT-2 was restricted to kidney and spleen. RNase protection revealed divergent transcription of WIT-1 and WIT-2, originating from a DNA region of less than 600 bp. Both transcripts were present at high concentrations in fetal kidney and at much reduced amounts in 5-year-old and adult kidneys. Eleven of 12 Wilms tumors classified as histopathologically heterogeneous exhibited absent or reduced expression of WIT-2, whereas only 4 of 14 histopathologically homogeneous tumors showed reduced expression. These data demonstrate a molecular basis for the pathogenetic heterogeneity in Wilms tumorigenesis.
Huang
A
A
Department of Genetics, Hospital for Sick Children, Toronto, Ontario, Canada.
Campbell
C E
CE
Bonetta
L
L
McAndrews-Hill
M S
MS
Chilton-MacNeill
S
S
Coppes
M J
MJ
Law
D J
DJ
Feinberg
A P
AP
Yeger
H
H
Williams
B R
BR
D001483:Base Sequence; D015152:Blotting, Northern; D004247:DNA / Q000235:genetics; D016162:Genes, Wilms Tumor / Q000235:genetics*; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D014158:Transcription, Genetic; D009396:Wilms Tumor / Q000235:genetics*
D004247:DNA