title: Chemoattractant-induced membrane hyperpolarization in Dictyostelium discoideum. A possible role for cyclic GMP.
abstract: Membrane potentials of the cellular slime mold Dictyostelium discoideum were monitored after chemotactic stimulation by measuring the distribution of the lipophilic cation tetraphenylphosphonium. Stimulation with the chemoattractant cAMP induces a transient membrane hyperpolarization which reaches its most negative value between 1-3 min after stimulation. This hyperpolarization is consistent with the opening of potassium channels. Measurements in streamer F mutant cells reveal that cGMP likely plays a role in the regulation of the cAMP-induced hyperpolarization.
authors.affiliation: Cell Biology and Genetics Unit, Leiden University, The Netherlands.
authors.forename: B
authors.initials: B
authors.lastname: Van Duijn
authors.forename: M
authors.initials: M
authors.lastname: Wang
mesh_terms: D002633:Chemotaxis; D000242:Cyclic AMP / Q000494:pharmacology; D006152:Cyclic GMP / Q000502:physiology*; D004023:Dictyostelium / Q000502:physiology*; D004054:Diethylstilbestrol / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D009154:Mutation; D011188:Potassium / Q000494:pharmacology; D011803:Quinine / Q000494:pharmacology; D013217:Starvation
chemical_list: D004054:Diethylstilbestrol; D011803:Quinine; D000242:Cyclic AMP; D006152:Cyclic GMP; D011188:Potassium