title: Recombinant human extracellular superoxide dismutase reduces concentration of oxygen free radicals in the reperfused rat heart.
abstract: OBJECTIVE
  The aim of the study was to determine whether recombinant human extracellular superoxide dismutase type C (rh-EC-SOD C) has antioxidant effects similar to those of the previously studied Cu-Zn superoxide dismutase.
  
  
  METHODS
  Electron paramagnetic resonance spectroscopy was used for quantification of free radical generation during reperfusion of postischaemic isolated rat hearts. The hearts were perfused with Krebs-Henseleit buffer and then subjected to 15 min global ischaemia. N-tert-butyl-alpha-phenylnitrone (PBN) was added as a spin trapping agent to the perfusate.
  
  
  METHODS
  18 hearts from Sprague-Dawley rats (280-400 g) were used.
  
  
  RESULTS
  Reperfusion resulted in a burst of radical formation. In presence of rh-EC-SOD C plus catalase, the PBN spin adduct concentration was only 18% (p less than 0.05) of the value in the control group. With bovine Cu-Zn superoxide dismutase plus catalase added to the buffer the corresponding spin adduct formation was 44% of control (NS = v rh-EC-SOD C).
  
  
  CONCLUSIONS
  In isolated rat heart, rh-EC-SOD C reduces free radical concentrations at least to the same extent as Cu-Zn superoxide dismutase.
authors.affiliation: Department of Cardiovascular Pharmacology, AB Hässle, Mölndal, Sweden.
authors.forename: M H
authors.initials: MH
authors.lastname: Johansson
authors.forename: J
authors.initials: J
authors.lastname: Deinum
authors.forename: S L
authors.initials: SL
authors.lastname: Marklund
authors.forename: P O
authors.initials: PO
authors.lastname: Sjöquist
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D006801:Humans; D066298:In Vitro Techniques; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000378:metabolism*; D009589:Nitrogen Oxides / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D003497:Cyclic N-Oxides; D005609:Free Radicals; D009589:Nitrogen Oxides; D011994:Recombinant Proteins; C029217:phenyl-N-tert-butylnitrone; D013482:Superoxide Dismutase; D010100:Oxygen