[Genetic analysis of the host range of the plasmid RSF1010].
32 host range mutants of pMO58, which contains a 8.0 kb fragment of RSF1010, were screened, using the transposon Tn5 insertion mutagenesis. To judge the genes which affect the host range of plasmid, the Tn5 insertion sites were located with restriction endonuclease mapping. The ability of the plasmid replication in different recipients was checked with DNA transformation. The results further confirmed the barrier to the host range of plasmid was their inability to replicate and maintain themselves in genetically different host. The repA repB and repC genes were necessary for the plasmid replication, but the repC gene may be a positive regulator and its function may be complemented by host gene products.
Sun
X
X
Department of Biology, Liaoning University, Shenyang.
D004261:DNA Replication; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D016297:Mutagenesis, Site-Directed; D010957:Plasmids / Q000235:genetics*; D011549:Pseudomonas / Q000235:genetics*
D004251:DNA Transposable Elements