Osteogenin inhibits proliferation and stimulates differentiation in mouse osteoblast-like cells (MC3T3-E1).
Osteogenin, a novel bone differentiation factor, was recently purified and characterized. We examined its effect on the proliferation and differentiation of MC3T3-E1 osteoblast-like cells. Cell proliferation was inhibited the first 48 h after addition of osteogenin, and this effect was independent of serum. Osteogenin did not influence the cell morphology. Alkaline phosphatase promptly increased in a dose and time-dependent manner and appeared to be specific. Treatment with TGF-beta 1 resulted in inhibition of alkaline phosphatase activity, and was reversed by osteogenin within 48 h. Cell cultures treated with osteogenin for 72 h after confluence became responsive to parathyroid hormone. Synthesis of collagenous proteins was stimulated by osteogenin. The present results demonstrate a significant influence of osteogenin on the differentiation of osteogenic phenotype in MC3T3-E1 cells in vitro.
Vukicevic
S
S
Bone Cell Biology Section, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892.
Luyten
F P
FP
Reddi
A H
AH
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001857:Bone Matrix / Q000166:cytology; D055398:Bone Morphogenetic Protein 3; D019485:Bone Morphogenetic Proteins; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D000242:Cyclic AMP / Q000378:metabolism; D066298:In Vitro Techniques; D051379:Mice; D010006:Osteoblasts / Q000166:cytology*; D010281:Parathyroid Hormone / Q000494:pharmacology; D011506:Proteins / Q000494:pharmacology*; D013997:Time Factors
D055398:Bone Morphogenetic Protein 3; D019485:Bone Morphogenetic Proteins; D010281:Parathyroid Hormone; D011506:Proteins; D000242:Cyclic AMP; D000469:Alkaline Phosphatase