Cloned manganese superoxide dismutase reduces oxidative stress in Escherichia coli and Anacystis nidulans.
The Mn superoxide dismutase gene of Escherichia coli was subcloned into the E. coli-Anacystis nidulans shuttle vector pSG111 to make the plasmid pMYG1. Transformation of E. coli HB101 with pMYG1 resulted in a 6-fold increase in superoxide dismutase activity. There was also induction of Mn superoxide dismutase in the transformants upon exposure to paraquat, as evidenced by dramatically increased levels of the Mn superoxide dismutase polypeptide in cytoplasmic extracts and a 16-fold further increase in superoxide dismutase activity. As well, the E. coli transformants showed resistance to paraquat-mediated inhibition of growth. Anacystis nidulans, a cyanobacterium that has no detectable Mn superoxide dismutase and is, consequently, very sensitive to oxidative stress, was also transformed with pMYG1. The transformants had detectable levels of Mn superoxide dismutase protein and showed resistance to paraquat-mediated inhibition of growth and photobleaching of pigments. Paraquat is known to promote formation of the superoxide radical anion, O2-., and thus the data have been interpreted as indicating that the cloned Mn superoxide dismutase provides protection in both E. coli and A. nidulans against damage attributable to O2-..
Gruber
M Y
MY
Department of Biology, University of Waterloo, ON, Canada.
Glick
B R
BR
Thompson
J E
JE
D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D015870:Gene Expression; D005798:Genes, Bacterial; D005822:Genetic Vectors; D010269:Paraquat / Q000494:pharmacology*; D010957:Plasmids; D015183:Restriction Mapping; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*
D013481:Superoxides; D013482:Superoxide Dismutase; D010269:Paraquat