Photosynthetic reaction center of green sulfur bacteria studied by EPR.
Membrane preparations of two species of the green sulfur bacteria Chlorobium have been studied by EPR. Three signals were detected which were attributed to iron-sulfur centers acting as electron acceptors in the photosynthetic reaction center. (1) A signal from a center designated FB, (gz = 2.07, gy = 1.91, gx = 1.86) was photoinduced at 4 K. (2) A similar signal, FA (gz = 2.05, gy = 1.94, gx = 1.88), was photoinduced in addition to the FB signal upon a short period of illumination at 200 K. (3) Further illumination at 200 K resulted in the appearance of a broad feature at g = 1.78. This is attributed to the gx component of an iron-sulfur center designated FX. The designations of these signals as FB, FA, and FX are based on their spectroscopic similarities to signals in photosystem I (PS I). The orientation dependence of these EPR signals in ordered Chlorobium membrane multilayers is remarkably similar to that of their PS I homologues. A magnetic interaction between the reduced forms of FB and FA occurs, which is also very similar to that seen in PS I. However, in contrast to the situation in PS I, FA and FB cannot be chemically reduced by sodium dithionite at pH 11. This indicates redox potentials for FA and FB which are lower by at least 150 mV than their PS I counterparts. The triplet state of P840, the primary electron donor, could be photoinduced at 4 K in samples which had been preincubated with sodium dithionite and methyl viologen and then preilluminated at 200 K.(ABSTRACT TRUNCATED AT 250 WORDS)
Nitschke
W
W
Service de Biophysique, Département de Biologie, CEN Saclay, Gif sur Yvette, France.
Feiler
U
U
Rutherford
A W
AW
D001426:Bacterial Proteins / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D006091:Gram-Negative Chemolithotrophic Bacteria / Q000032:analysis*; D007501:Iron / Q000378:metabolism; D008954:Models, Biological; D010084:Oxidation-Reduction; D045322:Photosynthetic Reaction Center Complex Proteins; D013696:Temperature
D001426:Bacterial Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D007501:Iron