Effect of rearing conditions on encephalomyocarditis (EMC) virus-induced diabetes in mice.
To compare the incidence in the diabetic syndrome in mice reared under sterile and non-sterile conditions, both conventional and SPF DBA/2 N male mice were inoculated intraperitoneally with two doses (10(4) TCID50/0.1 ml or 10(5) TCID50/0.1 ml) of encephalomyocarditis (EMC) virus M variant. The incidence of diabetic symptoms was higher in mice reared under sterile conditions than under non-sterile conditions. It appeared that a difference in animal rearing conditions would affect the sensitivity to EMC virus in mice.
Shichinohe
K
K
Department of Laboratory Animal Science, Nippon Medical School, Tokyo, Japan.
Shimizu
M
M
Asakawa
M
M
Ishizaki
M
M
D000822:Animal Husbandry; D000818:Animals; D000830:Animals, Laboratory; D003921:Diabetes Mellitus, Experimental / Q000209:etiology*; D004686:Encephalomyocarditis virus; D005856:Germ-Free Life; D008297:Male; D051379:Mice