Effects of bile salts on the adsorption of a hydrophobic mutagen to dietary fiber.
The effect of the bile salts, sodium cholate, deoxycholate, glycocholate and taurocholate, on the solubility in aqueous solution of the hydrophobic, environmental mutagen, 1,8-dinitropyrene (DNP), was examined. In the absence of bile salts, the DNP appeared to precipitate out of solution, whereas bile salts at a concentration of greater than or equal to 4 mM maintained the DNP in solution. In the presence of the model dietary fiber, alpha-cellulose, the DNP absorbed to this preferentially. Bile salts reduced this adsorption at low alpha-cellulose levels, but had little effect at high alpha-cellulose levels. The implication of these results is that bile salts have solubilising properties that could affect the distribution of hydrophobic molecules, including mutagens, in the digestive tract.
Ferguson
L R
LR
Cancer Research Laboratory, University of Auckland, New Zealand.
Harris
P J
PJ
Hollands
H J
HJ
Roberton
A M
AM
D000327:Adsorption; D001647:Bile Acids and Salts / Q000737:chemistry*; D002482:Cellulose; D019826:Cholic Acid; D002793:Cholic Acids / Q000737:chemistry; D003840:Deoxycholic Acid / Q000737:chemistry; D004043:Dietary Fiber; D006000:Glycocholic Acid / Q000737:chemistry; D007700:Kinetics; D009153:Mutagens / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry*; D012995:Solubility; D013656:Taurocholic Acid / Q000737:chemistry
D001647:Bile Acids and Salts; D002793:Cholic Acids; D004043:Dietary Fiber; D009153:Mutagens; D011721:Pyrenes; D003840:Deoxycholic Acid; C033310:1,8-dinitropyrene; D013656:Taurocholic Acid; D002482:Cellulose; D019826:Cholic Acid; D006000:Glycocholic Acid