Yeast SCO1 protein is required for a post-translational step in the accumulation of mitochondrial cytochrome c oxidase subunits I and II.
Biogenesis of functional cytochrome c oxidase in yeast requires the product of the nuclear gene SCO1. Strains deleted for this gene fail to accumulate the mitochondrially-synthesized cytochrome c oxidase subunits I and II, despite the presence of the respective mRNAs. Here we present data which demonstrate that the observed phenotype does not result from a failure to translate the mRNAs, but from a preferential degradation of the newly synthesized subunits. The SCO1 protein is therefore involved in a post-translational step in the accumulation of cytochrome c oxidase subunits I and II. We propose that the SCO1 protein is required for the correct assembly of both subunits into the cytochrome c oxidase complex.
Krummeck
G
G
Labor für Molekulare Biologie und Allgemeine Pathologie, Universität Ulm, München, Federal Republic of Germany.
Rödel
G
G
D003576:Electron Transport Complex IV / Q000378:metabolism*; D004586:Electrophoresis; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D008565:Membrane Proteins; D008928:Mitochondria / Q000201:enzymology*; D009154:Mutation; D010641:Phenotype; D011499:Protein Processing, Post-Translational; D014170:Transformation, Genetic; D015003:Yeasts / Q000201:enzymology / Q000235:genetics*
D005656:Fungal Proteins; D008565:Membrane Proteins; D003576:Electron Transport Complex IV