Genomic variation of herpes simplex virus type 2 isolates analysed by hybridization after electroblotting from polyacrylamide gels.
Genomic variation of herpes simplex virus type 2 (HSV-2) strains was analysed by polyacrylamide gradient gel electrophoresis and subsequent hybridization to cloned HSV-2 sequences. Two probes were used, one from the L-segment and one from the S-segment of the HSV-2 genome. The probes did not contain a-repeat sequences. Hybridization to the specific sequences in individual DNA fragments obtained by use of the frequently cleaving restriction endonuclease Alu I revealed variations in the genome not detectable by analysing the fragment size only. The use of 35S-labelled deoxynucleotide in the radioactive labelling of the probe further improved the resolution of the method.
Hukkanen
V
V
Department of Virology, University of Turku, Finland.
Ziegler
T
T
Kinnunen
L
L
Hirvonen
H
H
Auvinen
P
P
Hovi
T
T
D004279:DNA, Viral / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D018139:Simplexvirus / Q000235:genetics* / Q000302:isolation & purification
D004279:DNA, Viral