Biophysical studies on the morphology of baculovirus-expressed bluetongue virus tubules.
Bluetongue virus tubules were purified from Spodoptera frugiperda cells infected with a recombinant baculovirus containing the NS1 gene from bluetongue virus serotype 10, and expressed under control of the Autographa californica nuclear polyhedrosis virus polyhedrin promoter. These tubules were subjected to a variety of chemical and physical treatments and the resulting effects on tubule morphology were examined by electron microscopy. A number of morphological similarities were noted between bluetongue virus tubules and cellular microtubules despite a lack of homology between the component proteins at the primary sequence level. A possible multistranded helical configuration is proposed for the tubule structure.
Marshall
J J
JJ
NERC Institute of Virology and Environmental Microbiology, Oxford, U.K.
Fayard
B
B
Roy
P
P
D000818:Animals; D001820:Bluetongue virus / Q000032:analysis* / Q000235:genetics / Q000648:ultrastructure; D002213:Capsid / Q000032:analysis*; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D007181:Inclusion Bodies, Viral / Q000032:analysis / Q000648:ultrastructure*; D007304:Insect Viruses / Q000235:genetics; D008854:Microscopy, Electron; D009036:Moths; D012087:Reoviridae / Q000032:analysis*; D013696:Temperature; D014758:Viral Core Proteins / Q000032:analysis*; D017361:Viral Nonstructural Proteins
D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins