Alpha-amidation of gastrin is impaired by diethyldithiocarbamate.
The influence of gastrin alpha-amidation of the heavy-metal chelator diethyldithiocarbamate and disulfiram, its disulfide dimer, was studied in rat gastric antrum. Sensitive, sequence-specific immunoassays for glycine-extended and amidated gastrin were used to monitor extractions and chromatography. The results showed that intraperitoneal diethyldithiocarbamate administration (1000 mg/kg body weight) for two days caused a decrease in amidated gastrin from 2.6 +/- 0.4 to 1.4 +/- 0.3 nmol/g tissue (n = 11) with a simultaneous increase in glycine-extended gastrin from 0.84 +/- 0.15 to 2.4 +/- 0.3 nmol/g. Peroral administration of disulfiram (4 mg/kg body weight) for nine days did not change alpha-amidation significantly. The results of the present study demonstrate that the heavy-metal chelating agent diethyldithiocarbamate inhibits alpha-amidation of gastrin in vivo, in agreement with the inhibition of amidating activity observed in vitro. These results are in accordance with the previous observations that the presence of copper ions is necessary for the alpha-amidation to take place.
Hilsted
L
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University Department of Clinical Chemistry, Rigshospitalet, Copenhagen, Denmark.
D000577:Amides / Q000378:metabolism*; D000818:Animals; D002850:Chromatography, Gel; D003300:Copper / Q000502:physiology; D004221:Disulfiram / Q000494:pharmacology; D004050:Ditiocarb / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000378:metabolism; D005755:Gastrins / Q000378:metabolism*; D008297:Male; D011499:Protein Processing, Post-Translational / Q000187:drug effects*; D011706:Pyloric Antrum / Q000187:drug effects / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013270:Stomach / Q000187:drug effects*
D000577:Amides; D005755:Gastrins; D003300:Copper; D004050:Ditiocarb; D004221:Disulfiram