Comparison of biological activities of endothelin-1, -2 and -3 in murine and human fibroblast cell lines.
The effects of three forms of endothelin (ET), ET-1, -2 and -3, on intracellular free Ca2+ concentration ([Ca2+]i) and their receptor binding activities have been compared in murine fibroblast cell line Swiss 3T3 as well as diploid human fibroblast cell line FS-4. In both cell lines, ET-1 and -2 induced a profound increase in [Ca2+]i with a similar dose-response relationship; ET-3 was far less potent. ET-1 and -2 showed specific binding to both cell lines with similar dissociation constants and numbers of binding sites. Negligible specific binding of ET-3 was detected. These findings indicate that these two fibroblast populations possess high affinity receptors for ET-1 and -2 and practically no ET-3 receptors for ET-3, and binding of ET-1 and -2 increases [Ca2+]i in these cell lines.
Ohnishi-Suzaki
A
A
Growth Factor Division, National Cancer Center Research Institute, Tokyo, Japan.
Yamaguchi
K
K
Kusuhara
M
M
Adachi
I
I
Abe
K
K
Kimura
S
S
D000818:Animals; D001667:Binding, Competitive; D002118:Calcium / Q000378:metabolism; D016232:Endothelins; D005347:Fibroblasts; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D010455:Peptides / Q000145:classification / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000145:classification / Q000502:physiology*; D017466:Receptors, Endothelin; D015398:Signal Transduction
D016232:Endothelins; D010455:Peptides; D011956:Receptors, Cell Surface; D017466:Receptors, Endothelin; D002118:Calcium