title: Completion of the primary structure of the human type IV collagenase preproenzyme and assignment of the gene (CLG4) to the q21 region of chromosome 16.
abstract: The complete amino acid sequence of the human type IV collagenase preproenzyme was determined from cDNA and genomic clones. Primer extension and S1 nuclease analyses as well as nucleotide sequencing of a genomic clone indicate that the first exon has two closely spaced initiation sites for transcription and codes for 290 and 280 nt of a 5' untranslated region and a 29-residue signal peptide. The gene (CLG4) was localized to 16q21 using somatic cell hybrids and in situ hybridization.
authors.affiliation: Biocenter, University of Oulu, Finland.
authors.forename: P
authors.initials: P
authors.lastname: Huhtala
authors.forename: R L
authors.initials: RL
authors.lastname: Eddy
authors.forename: Y S
authors.initials: YS
authors.lastname: Fan
authors.forename: M G
authors.initials: MG
authors.lastname: Byers
authors.forename: T B
authors.initials: TB
authors.lastname: Shows
authors.forename: K
authors.initials: K
authors.lastname: Tryggvason
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16; D017364:Collagenases; D004247:DNA / Q000235:genetics; D004792:Enzyme Precursors / Q000235:genetics*; D005796:Genes; D006801:Humans; D006822:Hybrid Cells / Q000032:analysis; D051379:Mice; D003012:Microbial Collagenase / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D021382:Protein Sorting Signals / Q000235:genetics
chemical_list: D004792:Enzyme Precursors; D021382:Protein Sorting Signals; D004247:DNA; D017364:Collagenases; D003012:Microbial Collagenase