Molecular identification of the human tumor necrosis factor receptor on interleukin-2-stimulated peripheral blood lymphocytes.
The human tumor necrosis factor (TNF) receptor on interleukin (IL)-2-stimulated lymphocytes was characterized by binding and crosslinking techniques. The TNF receptor on IL-2-activated lymphocytes has an affinity of approximately 50 pM. Conventional crosslinking studies with the DSS analog bis(sulfosuccinimidyl) suberate demonstrated a ligand-receptor complex molecular weight of 106-108 kDa. Lectin precipitation experiments indicated that the receptor is a glycoprotein with an affinity for lectin isolated from Ricinus communis. Affinity crosslinking studies with the iodinateable, cleavable crosslinker sulfosuccinimidyl 2-(p-azido-salicylamido) ethyl 1,3'-dithiopropionate demonstrated that the TNF receptor, by itself, in the absence of bound ligand, has a molecular weight of approximately 90 kDa. Furthermore, these results indicate that the crosslinked TNF:TNF-receptor complexes observed at 104-108 kDa are composed of receptor and monomeric TNF.
Crump
W L
WL
Department of Tumor Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Owen-Schaub
L B
LB
Grimm
E A
EA
D000345:Affinity Labels; D011232:Chemical Precipitation; D003432:Cross-Linking Reagents; D006801:Humans; D007376:Interleukin-2 / Q000502:physiology; D037102:Lectins; D008027:Light; D008214:Lymphocytes / Q000737:chemistry*; D008970:Molecular Weight; D011956:Receptors, Cell Surface / Q000302:isolation & purification*; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000345:Affinity Labels; D003432:Cross-Linking Reagents; D007376:Interleukin-2; D037102:Lectins; D011956:Receptors, Cell Surface; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha