Enzymatic and non-enzymatic formation of free radicals from aflatoxin B1.
Formation of free radicals from aflatoxin B1 was demonstrated in systems of a) NADPH/microsome, b) H2O2/peroxidase, c) UV irradiation, d) silver oxide oxidation and e) alkaline dimethyl sulfoxide. In a) to c), radical spots were detected in thin-layer chromatograms. Among several known metabolites of aflatoxin B1, aflatoxin M1 was active in generation of free radical. These results suggest a possible role of free radical metabolites in the cytotoxicity of this hepatocarcinogen.
Kodama
M
M
Biophysics Division, National Cancer Center Research Institute, Tokyo, Japan.
Inoue
F
F
Akao
M
M
D016604:Aflatoxin B1; D000348:Aflatoxins / Q000378:metabolism* / Q000528:radiation effects; D000818:Animals; D002273:Carcinogens / Q000378:metabolism* / Q000528:radiation effects; D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D006735:Horseradish Peroxidase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D010087:Oxides / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012834:Silver / Q000494:pharmacology; D018030:Silver Compounds; D014466:Ultraviolet Rays
D000348:Aflatoxins; D002273:Carcinogens; D005609:Free Radicals; D010087:Oxides; D018030:Silver Compounds; D012834:Silver; D009249:NADP; C040225:disilver oxide; D016604:Aflatoxin B1; D006735:Horseradish Peroxidase