Rate enhancement of the electron transfer of the adrenodoxin-adrenodoxin reductase system by inorganic and nucleotide phosphates.
Phosphate and pyrophosphate increased the rate of reduction of adrenodoxin by NADPH-adrenodoxin reductase and NADPH, pyrophosphate being one order more effective than the former. However, the cytochrome c reduction by the electron transport system was inhibited in the presence of inorganic (pyro)phosphate. On the other hand, ADP and ATP enhanced the rates of reduction of both adrenodoxin and cytochrome c through adrenodoxin by the electron transport system. GTP also enhanced the rate of reduction of cytochrome c by this system, whereas AMP showed no appreciable enhancement. These inorganic and nucleotide phosphates did not affect the rate of ferricyanide reduction by the reductase.
Yamano
T
T
Laboratory of Chemistry, Kansai Medical University, Hirakata, Japan.
Nonaka
Y
Y
Miura
R
R
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000302:Adrenal Cortex / Q000201:enzymology; D000325:Adrenodoxin / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D003574:Cytochrome c Group / Q000378:metabolism; D011756:Diphosphates / Q000494:pharmacology; D004579:Electron Transport / Q000187:drug effects; D005287:Ferredoxin-NADP Reductase / Q000378:metabolism*; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D010710:Phosphates / Q000494:pharmacology*; D012265:Ribonucleotides / Q000494:pharmacology*
D003574:Cytochrome c Group; D011756:Diphosphates; D010710:Phosphates; D012265:Ribonucleotides; D000325:Adrenodoxin; D009249:NADP; D000255:Adenosine Triphosphate; D005287:Ferredoxin-NADP Reductase; D009247:NADH, NADPH Oxidoreductases