Adaptation of the slide immunoenzymatic assay for quantification of DNA hybridization: SIA-DNA.
A quantitative, non-radioisotopic microsystem has been developed for measuring nucleic acid hybridization using microliter volumes of test sample and reagents. This new method, Slide Immunoenzymatic Assay-DNA, is a modification of the Slide Immunoenzymatic Assay technology originally designed for quantifying antigens and antibodies. It features small, circular solid phases (circles) of transparent material for nucleic acid immobilization. This allows the use of enzyme-labelled gene probes and substrates that generate color which, due to the distribution pattern of the circles on their support, can be measured by automated microtitration plate readers. Slide Immunoenzymatic Assay-DNA has been standardized to measure hybridization of probe to purified DNA or to DNA in cells lysed directly on the circles. Owing to its simplicity, relative low cost and expeditiousness, i.e., providing results in four hours. Slide Immunoenzymatic Assay-DNA is also suitable for use in simple laboratories and field studies.
Conway de Macario
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Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany, NY 12201-0509.
Jovell
R J
RJ
Macario
A J
AJ
D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D009693:Nucleic Acid Hybridization; D010957:Plasmids
D015342:DNA Probes; D004269:DNA, Bacterial