title: Identification of Campylobacter jejuni surface proteins that bind to Eucaryotic cells in vitro.
abstract: To understand the role of Campylobacter jejuni surface proteins in the interaction of C. jejuni with cultured mammalian cell lines in vitro, we developed a ligand-binding assay. This procedure allowed us to antigenically identify C. jejuni outer membrane proteins (OMPs) that attach to intact host cell membranes. OMPs isolated from an invasive strain and a less invasive strain were antigenically indistinguishable. However, we found that proteins with molecular masses of 28 and 32 kilodaltons (kDa) from just the invasive strain bound to HEp-2 cell monolayers. Binding of the 32-kDa OMP was cell line specific and correlated directly with the ability of the invasive C. jejuni strain to penetrate. Such a correlation was probably also true for the 28-kDa OMP. We also investigated the binding of glycine acid extracts with cell line HEp-2. We identified four proteins with apparent molecular masses of 28, 32, 36, and 42 kDa in the invasive strain extracts that bound to HEp-2 cells. In contrast, only the 36-kDa protein from the less invasive strain bound to HEp-2 cells. Our data suggest that binding of these surface exposed proteins may play a key role in C. jejuni-host cell interactions and ultimate invasion.
authors.affiliation: Département de Microbiologie, Centre Médical Universitaire, Geneva, Switzerland.
authors.forename: M A
authors.initials: MA
authors.lastname: de Melo
authors.forename: J C
authors.initials: JC
authors.lastname: Pechère
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D002168:Campylobacter fetus / Q000378:metabolism* / Q000472:pathogenicity; D002458:Cell Fractionation; D002478:Cells, Cultured / Q000382:microbiology; D006224:Cricetinae; D004848:Epithelium / Q000382:microbiology; D006801:Humans; D008970:Molecular Weight; D011956:Receptors, Cell Surface / Q000378:metabolism
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D011956:Receptors, Cell Surface