Chromosome banding analysis of a new stemline of hypodiploid Hirosaki sarcoma established by single cell transplantation.
A new hypodiploid stemline of the long-lasting ascites sarcoma, Hirosaki sarcoma (HS), was established by means of single-cell transplantation, and its G-, C- and NOR-banding patterns were compared with those of the predecessor stemline, with special notice taken of the structural alteration of marker chromosomes. Their chromosomal relationship was well explained by the rearrangement, t(M7pM8q): Translocation of the short arm of marker M7 to the NOR of marker M8 produced a new marker M8t, and the translocation-carrying variant cells grew into a subline. A new translocation-carrying stemline was formed from this subline by single-cell transplantation. Judging from the size and number of silver-positive NORs, the NOR activity was significantly higher in this stemline than in its predecessor. The increased NOR activity might have been partly responsible for the growth of a translocation-carrying variant cell into a new subline. Single-cell-mass transplantation may be one of the most effective systems for studying the proliferative ability of subline cells and for understanding the mechanism of stemline formation in transplantable rat ascites sarcomas.
Obara
Y
Y
Department of Biology, Faculty of Science, Hirosaki University, Japan.
Mizui
K
K
Sato
T
T
D000818:Animals; D002460:Cell Line; D017690:Cell Transplantation; D002871:Chromosome Banding; D005819:Genetic Markers; D007621:Karyotyping; D014411:Neoplastic Stem Cells / Q000648:ultrastructure*; D009697:Nucleolus Organizer Region / Q000276:immunology / Q000502:physiology; D011003:Ploidies; D051381:Rats; D012509:Sarcoma / Q000235:genetics*; D014178:Translocation, Genetic
D005819:Genetic Markers