title: Dynamic association between artery shear flow condition and platelet cytosolic free Ca2+ concentration in human hypertension.
abstract: 1. Blood cells and vascular endothelial cells are subjected to a wide range of haemodynamically generated shear stress forces. In vitro, membrane stretching or shear stress have been observed to activate ion channels and cell metabolism and to facilitate erythrocyte and platelet aggregation. 2. The present study was designed to evaluate the participation of shear stresses in the control of apparent platelet cytosolic free Ca2+ concentration in hypertensive patients. 3. Shear conditions and platelet cytosolic free Ca2+ concentration in vitro were studied after a dynamic perturbation induced by 3 months of double-blind treatment with one of two beta-antagonists, carteolol and atenolol. Brachial artery wall shear rate and stress were estimated by means of a pulsed Doppler velocimeter, and blood viscosity was measured by a co-axial viscometer at a shear rate of 96 s-1. Platelet cytosolic free Ca2+ concentration was simultaneously measured by using the Quin-2 fluorescent chelator. The direct effect of atenolol and carteolol on platelet cytosolic free Ca2+ concentration in vitro was also measured after addition of the beta-blockers to platelet-rich plasma. 4. Atenolol and carteolol decreased blood pressure similarly but their effects on shear rate (P less than 0.02), shear stress (P less than 0.01) and platelet cytosolic free Ca2+ concentration (P less than 0.05) differed after 3 months of therapy. In contrast, neither of the drugs significantly altered platelet cytosolic free Ca2+ concentration, in vitro per se. 5. In the overall population, strong positive correlations existed not only between changes in platelet cytosolic free Ca2+ concentration and those in shear rate (r = 0.81, P less than 0.001) and shear stress (r = 0.83, P less than 0.001), but also between their absolute values, suggesting a possible haemodynamic shear-dependent modulation of transmembrane Ca2+ transport.
authors.affiliation: INSERUM U 28, Centre de Diagnostic, Hôpital Broussais, paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Levenson
authors.forename: M A
authors.initials: MA
authors.lastname: Devynck
authors.forename: I
authors.initials: I
authors.lastname: Pithois-Merli
authors.forename: K H
authors.initials: KH
authors.lastname: Le Quan Sang
authors.forename: V
authors.initials: V
authors.lastname: Filitti
authors.forename: A
authors.initials: A
authors.lastname: Simon
mesh_terms: D001158:Arteries / Q000503:physiopathology*; D001262:Atenolol / Q000627:therapeutic use; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002354:Carteolol / Q000627:therapeutic use; D003600:Cytosol / Q000378:metabolism; D004311:Double-Blind Method; D005260:Female; D006439:Hemodynamics; D006801:Humans; D006973:Hypertension / Q000188:drug therapy / Q000503:physiopathology*; D008297:Male; D012212:Rheology; D013314:Stress, Mechanical
chemical_list: D001262:Atenolol; D002354:Carteolol; D002118:Calcium