title: Antibody response of the ovine lymph node to experimental infection with an ovine abortion strain of Chlamydia psittaci.
abstract: After primary infection with Chlamydia psittaci in the draining area of the popliteal lymph node, viable organisms could be isolated from the efferent lymph only before the primary immune response developed. The lymph antibody response, as assayed by the complement fixation and immunofluorescence (IF) antibody tests, showed a rise in titre that peaked approximately 2 weeks after infection. Immunoblotting revealed that antibodies produced during this period were predominantly directed against the major outer membrane protein (MOMP). In secondary infection of convalescent sheep, an elevated IF antibody titre, already present in the lymph and blood, could not be boosted. Viable organisms could not be isolated from these sheep. Antibodies produced reacted to 12-14 bands in the immunoblot profile including the MOMP band. These potentially immunoprotective antigens, particularly MOMP, should be considered as useful candidates for an improved vaccine against ovine enzootic abortion, in further investigations.
authors.affiliation: Moredun Research Institute, Edinburgh, Gt. Britain.
authors.forename: H S
authors.initials: HS
authors.lastname: Huang
authors.forename: T W
authors.initials: TW
authors.lastname: Tan
authors.forename: D
authors.initials: D
authors.lastname: Buxton
authors.forename: I E
authors.initials: IE
authors.lastname: Anderson
authors.forename: A J
authors.initials: AJ
authors.lastname: Herring
mesh_terms: D000034:Abortion, Veterinary / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D002691:Chlamydophila psittaci / Q000276:immunology; D003168:Complement Fixation Tests; D005260:Female; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D008198:Lymph Nodes / Q000276:immunology*; D011247:Pregnancy; D009956:Psittacosis / Q000276:immunology / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins