Immunogenicity of herpes simplex virus type 1 glycoproteins expressed in vaccinia virus recombinants.
Vaccinia virus recombinants expressing glycoproteins B (vgB11), D (VgD52), E (gE/7.5 and gE/4B), G (gG-vac), H (gH-vac), and I (gI-vac) of HSV-1 were used to compare the protective response to these individual glycoproteins in the mouse. Glycoprotein D induced the best neutralizing antibody titers and the most increased rates of HSV clearance from the ear as well as good protection from the establishment of latent HSV infections in the sensory ganglia. Glycoprotein B also induced good neutralizing antibody titers and as great a protection from the establishment of latency as gD although the rate of virus clearance from the ear was not as great as after immunization with gD. Glycoprotein E induced weak neutralizing antibody but gG, gH, and gI did not show a neutralizing antibody response. At higher challenge doses of virus (10(6) PFU HSV-1 in the ear), gE induced a protective response by increasing the rate of virus clearance and reducing the acute infection of ganglia as compared to negative control immunized mice. However there was no protection from the establishment of latent infections after immunization with gE. No protective response was seen to gG, gH, or gl.
Blacklaws
B A
BA
Department of Pathology, University of Cambridge, United Kingdom.
Krishna
S
S
Minson
A C
AC
Nash
A A
AA
D000818:Animals; D000917:Antibody Formation; D002460:Cell Line; D004423:Ear / Q000382:microbiology; D005260:Female; D005728:Ganglia, Sympathetic / Q000382:microbiology; D006561:Herpes Simplex / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D009500:Neutralization Tests; D011995:Recombination, Genetic; D018139:Simplexvirus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D014616:Vaccinia virus / Q000235:genetics; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
D014759:Viral Envelope Proteins