Heterogeneous expression of poliovirus receptor-related proteins in human cells and tissues.
Portions of the cellular receptor for poliovirus were expressed in Escherichia coli as fusion proteins with the product of the trpE gene. One of two antireceptor antisera obtained by immunizing rabbits with the fusion proteins blocked poliovirus infection. Western immunoblot analyses demonstrated that poliovirus receptor-related proteins were expressed in HeLa cells and a variety of human tissues, including those that are not sites of poliovirus replication. Tissue-specific variation in electrophoretic mobility, immunoreactivity, and subunit arrangement of poliovirus receptor-related proteins was observed. These results demonstrate that poliovirus tissue tropism cannot be explained by a limited distribution of receptor polypeptide, but may be the result of alternative splicing, posttranslational modifications, or both. In addition, the widespread but heterogeneous expression of the receptor suggests that the protein may have an important endogenous function.
Freistadt
M S
MS
Department of Microbiology, Columbia University College of Physicians & Surgeons, New York, New York 10032.
Kaplan
G
G
Racaniello
V R
VR
D015153:Blotting, Western; D002472:Cell Transformation, Viral; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D006367:HeLa Cells / Q000502:physiology; D006801:Humans; D007106:Immune Sera; D008565:Membrane Proteins; D017955:Poliovirus / Q000235:genetics / Q000502:physiology*; D011991:Receptors, Virus / Q000032:analysis / Q000235:genetics* / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000378:metabolism
D007106:Immune Sera; D008565:Membrane Proteins; D011991:Receptors, Virus; D011993:Recombinant Fusion Proteins; C066100:poliovirus receptor