Strand scission in DNA by quercetin and Cu(II): identification of free radical intermediates and biological consequences of scission.
Quercetin was shown to reduce oxygen to superoxide. In the presence of Cu(II), the hydroxyl radical was formed. The strand scission of DNA was shown to occur under conditions in which Cu(II), quercetin and either hydrogen peroxide or oxygen were present and superoxide was not a necessary intermediate. Strand scission involved the hydroxyl radical and a radical DNA intermediate. The strand scission reaction was shown to account for the biological activity of quercetin as assayed by bacteriophage inactivation.
Fazal
F
F
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, India.
Rahman
A
A
Greensill
J
J
Ainley
K
K
Hadi
S M
SM
Parish
J H
JH
D003300:Copper / Q000378:metabolism*; D004247:DNA / Q000187:drug effects / Q000378:metabolism*; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D011794:Quercetin / Q000378:metabolism* / Q000633:toxicity; D013481:Superoxides / Q000378:metabolism
D004277:DNA, Single-Stranded; D013481:Superoxides; D003300:Copper; D004247:DNA; D011794:Quercetin; D006861:Hydrogen Peroxide