title: Induction of a human carbonyl reductase gene located on chromosome 21.
abstract: Carbonyl reductase (EC 1.1.1.184) belongs to the group of enzymes called aldo-keto reductases. It is a NADPH-dependent cytosolic protein with specificity for many carbonyl compounds including the antitumor anthracycline antibiotics, daunorubicin and doxorubicin. Human carbonyl reductase was cloned from a breast cancer cell line (MCF-7). The cDNA clone contained 1219 base paires with an open reading frame corresponding to 277 amino acids encoding a protein of Mr 30,375. Southern analysis of genomic DNA digested with several restriction enzymes and analyzed by hybridization with a labeled cDNA probe indicated that carbonyl reductase is probably coded by a single gene and does not belong to a family of structurally similar enzymes. Southern analysis of 17 mouse/human somatic cell hybrids showed that carbonyl reductase is located on chromosome 21. Carbonyl reductase mRNA could be induced 3-4-fold in 24 h with 10 microM 2,(3)-t-butyl-4-hydroxyanisole (BHA), beta-naphthoflavone or Sudan 1.
authors.affiliation: Division of Biology, Beckman Research Institute at the City of Hope, Duarte, CA 91010.
authors.forename: G L
authors.initials: GL
authors.lastname: Forrest
authors.forename: S
authors.initials: S
authors.lastname: Akman
authors.forename: S
authors.initials: S
authors.lastname: Krutzik
authors.forename: R J
authors.initials: RJ
authors.lastname: Paxton
authors.forename: R S
authors.initials: RS
authors.lastname: Sparkes
authors.forename: J
authors.initials: J
authors.lastname: Doroshow
authors.forename: R L
authors.initials: RL
authors.lastname: Felsted
authors.forename: C J
authors.initials: CJ
authors.lastname: Glover
authors.forename: T
authors.initials: T
authors.lastname: Mohandas
authors.forename: N R
authors.initials: NR
authors.lastname: Bachur
mesh_terms: D000429:Alcohol Oxidoreductases / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D000449:Aldehyde Reductase; D000074408:Aldo-Keto Reductases; D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D002891:Chromosomes, Human, Pair 21; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004790:Enzyme Induction; D015971:Gene Expression Regulation, Enzymologic; D015723:Gene Library; D005796:Genes; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D004247:DNA; D000429:Alcohol Oxidoreductases; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase; C000614209:AKR7A5 protein, mouse