Electron spectroscopic imaging (ESI) of viruses using thin-section and immunolabelling preparations.
Electron spectroscopic imaging (ESI) and conventional bright-field transmission electron microscopy (TEM) were applied comparatively for the analysis of the fine structure and the antigenic make-up of human immunodeficiency virus and two herpes viruses. In addition to the information obtained in conventional bright-field TEM, ESI leads to high-contrast imaging of ultrathin sections and improves the resolution of thin and thick sections, and allows a better detectability of the immunolabelling markers.
Ozel
M
M
Robert Koch-Institut des Bundesgesundheitsamtes, Berlin, Germany.
Pauli
G
G
Gelderblom
H R
HR
D000956:Antigens, Viral / Q000032:analysis; D015488:HIV Antigens / Q000032:analysis; D015497:HIV-1 / Q000276:immunology / Q000648:ultrastructure*; D015654:Herpesvirus 6, Human / Q000276:immunology / Q000648:ultrastructure*; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D018139:Simplexvirus / Q000276:immunology / Q000648:ultrastructure*; D013057:Spectrum Analysis
D000956:Antigens, Viral; D015488:HIV Antigens