Structural features associated with alpha- anomeric in oligo-alpha-thymidylates.
Comparison between gel electrophoresis migrations of oligo-alpha-thymidylates and oligo-beta-thymidylates indicates that the migration of alpha-oligonucleotides under native conditions is different from the migration of beta-oligonucleotides when the number of thymines, part of the sequence, is higher than 5. Such difference disappears when the gels are run under denaturing conditions. This, together with UV spectra, indicates that the structure of alpha-oligonucleotides is more organized than the structure of beta-oligonucleotides and that such an organisation appears for a length higher than 5 monomeric units.
Bertrand
J R
JR
Unité de Biochimie, URA 158 CNRS, Villejuif, France.
Bazile
D
D
Malvy
C
C
Paoletti
J
J
D004591:Electrophoresis, Polyacrylamide Gel; D007536:Isomerism; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D010727:Phosphoric Diester Hydrolases; D010761:Phosphorus Radioisotopes; D013056:Spectrophotometry, Ultraviolet
D009838:Oligodeoxyribonucleotides; D010761:Phosphorus Radioisotopes; C027903:oligo (dT); D010727:Phosphoric Diester Hydrolases