Bending and supercoiling of DNA at the attachment site of bacteriophage lambda.
Integration of the DNA of bacteriophage lambda into the chromosome of E. coli depends on the formation of a complex nucleoprotein array at a specific locus on the phage genome, the attachment site. Recent work shows how bending of this DNA (induced by a specific DNA-binding protein), and strain in this DNA (induced by supercoiling) contribute to the formation of the nucleoprotein structure. Further, there are new insights into the way this structure directs critical events during recombination.
Nash
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Laboratory of Molecular Biology, National Institute of Mental Health, Bethesda, MD 20892.
D001287:Attachment Sites, Microbiological; D001426:Bacterial Proteins / Q000235:genetics; D010582:Bacteriophage lambda / Q000201:enzymology / Q000235:genetics*; D004254:DNA Nucleotidyltransferases / Q000235:genetics* / Q000378:metabolism; D004251:DNA Transposable Elements; D004278:DNA, Superhelical / Q000502:physiology*; D004279:DNA, Viral / Q000502:physiology*; D004926:Escherichia coli; D019426:Integrases; D034982:Integration Host Factors; D008242:Lysogeny; D008958:Models, Molecular; D009690:Nucleic Acid Conformation
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004278:DNA, Superhelical; D004279:DNA, Viral; D034982:Integration Host Factors; D004254:DNA Nucleotidyltransferases; D019426:Integrases