title: Down regulation by p60v-src of genes specifically expressed and developmentally regulated in postmitotic quail neuroretina cells.
abstract: The avian neuroretina (NR) is composed of photoreceptors and different neurons that are derived from proliferating precursor cells. Neuronal differentiation takes place after terminal mitosis. We have previously shown that differentiating NR cells can be induced to proliferate by infection with Rous sarcoma virus (RSV) and that cell multiplication requires expression of a functional v-src gene. We speculated that the quiescence of NR cells could be determined by specific genes. Cell proliferation could then result from the negative regulation of these genes by the v-src protein. By differential hybridization of a cDNA library, we isolated eight clones corresponding to genes expressed in postmitotic NR cells from 13-day-old quail embryos, transcriptional levels of which are significantly reduced in NR cells induced to proliferate by tsNY68, an RSV mutant with temperature-sensitive mitogenic activity. Partial sequencing analysis indicated that one RNA encoded the calmodulin gene, whereas the other seven showed no similarity to known sequences. By using v-src mutants that induce NR cell proliferation in the absence of transformation, we showed that transcription of six genes was negatively regulated by the v-src protein and that of four genes was correlated with NR cell quiescence. We also report that a subset of genes are specifically transcribed in neural cells and developmentally regulated in the NR. These results indicate that the v-src protein regulates expression of genes likely to play a role in the control of neural cell growth or differentiation.
authors.affiliation: Institut Curie--Biologie, Orsay, France.
authors.forename: M
authors.initials: M
authors.lastname: Guermah
authors.forename: G
authors.initials: G
authors.lastname: Gillet
authors.forename: D
authors.initials: D
authors.lastname: Michel
authors.forename: D
authors.initials: D
authors.lastname: Laugier
authors.forename: G
authors.initials: G
authors.lastname: Brun
authors.forename: G
authors.initials: G
authors.lastname: Calothy
mesh_terms: D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D003001:Cloning, Molecular; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D004625:Embryo, Nonmammalian; D005784:Gene Amplification; D015967:Gene Expression Regulation, Viral; D015723:Gene Library; D008938:Mitosis; D009154:Mutation; D015688:Oncogene Protein pp60(v-src) / Q000235:genetics*; D009857:Oncogenes; D009928:Organ Specificity; D010786:Photoreceptor Cells / Q000166:cytology*; D011784:Quail; D012160:Retina / Q000166:cytology*; D012165:Retinal Ganglion Cells / Q000166:cytology*
chemical_list: D015342:DNA Probes; D004279:DNA, Viral; D015688:Oncogene Protein pp60(v-src)