title: In vitro interactions of cytokines and bovine viral diarrhea virus in phytohemagglutinin-stimulated bovine mononuclear cells.
abstract: Bovine viral diarrhea (BVD) virus inhibited phytohemagglutinin (PHA)-stimulated bovine peripheral blood mononuclear cell (PBMC) proliferation and bovine interleukin-2 (IL-2) production. In the controls, the heat-inactivated BVD virus was not capable of suppressing the PHA-stimulated PBMC proliferation. Presence of exogenous cytokines, such as purified human IL-2, recombinant bovine interleukin-1 (rbovIL-1), recombinant bovine IL-2, and recombinant human IL-6 failed to reverse the BVD virus-induced immunosuppression. Also, we found that the BVD virus inhibited PHA and IL-2 induced proliferation of bovine PBMC in the early and late stages of activation. In summary, our data suggest that BVD virus induced immunosuppression was not due to destruction of the PBMC but may be inhibiting one or more of the important intracellular enzymes that may regulate PBMC proliferation.
authors.affiliation: Department of Anatomy, Kansas State University, Manhattan 66506.
authors.forename: D
authors.initials: D
authors.lastname: Atluru
authors.forename: W
authors.initials: W
authors.lastname: Xue
authors.forename: S
authors.initials: S
authors.lastname: Polam
authors.forename: S
authors.initials: S
authors.lastname: Atluru
authors.forename: F
authors.initials: F
authors.lastname: Blecha
authors.forename: H C
authors.initials: HC
authors.lastname: Minocha
mesh_terms: D000818:Animals; D001685:Biological Factors / Q000494:pharmacology*; D002417:Cattle / Q000276:immunology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology*; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D006801:Humans; D007108:Immune Tolerance; D007376:Interleukin-2 / Q000096:biosynthesis / Q000494:pharmacology; D007378:Interleukins / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism*; D008213:Lymphocyte Activation; D010576:Pestivirus / Q000276:immunology*; D010835:Phytohemagglutinins / Q000276:immunology; D011994:Recombinant Proteins
chemical_list: D001685:Biological Factors; D016207:Cytokines; D007376:Interleukin-2; D007378:Interleukins; D010835:Phytohemagglutinins; D011994:Recombinant Proteins