Blocking by anti-idiotypic antibodies of monoclonal antibody-mediated protection against lethal Semliki Forest virus in mice.
Semliki Forest virus-(SEV) neutralizing monoclonal antibodies (MoAbs), produced after fusion of spleen cells from BALB/c mice and myeloma cell line P3-X63-AG8. 653 or SP2/0, were used for anti-idiotypic immunization of female BALB/c mice. Two intracutaneous immunizations (2 x 40 micrograms per animal), 3 weeks apart, with keyhole limpet haemocyanin-conjugated MoAbs mixed with the saponin Quil A were sufficient to induce high levels of anti-idiotypic antibodies in the circulation of these mice with the capacity to block specifically in vitro MoAb-mediated virus neutralization. Anti-idiotypic antibodies against SFV-neutralizing MoAbs, either passively transferred or actively acquired by immunization, are also able to abrogate (specifically) passive immunity, mediated by critical protective doses of MoAb, in mice against infection with a lethal strain of SFV. Furthermore we confirmed by intervention with anti-idiotypic serum in vivo that an SFV-neutralizing MoAb exerts its greatest protective effect during the first 2 days of infection.
Oosterlaken
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TA
Institute for Molecular Biology and Medical Biotechnology, Eykman-Winkler Laboratorium for Medical Microbiology, Utrecht, The Netherlands.
Harmsen
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M
Kraaijeveld
C A
CA
Snippe
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H
D000276:Adjuvants, Immunologic; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000008:administration & dosage* / Q000276:immunology; D005260:Female; D006433:Hemocyanins / Q000276:immunology; D007116:Immunization, Passive; D007124:Immunoenzyme Techniques; D007739:L Cells; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012672:Semliki forest virus / Q000276:immunology; D014036:Togaviridae Infections / Q000276:immunology* / Q000401:mortality / Q000517:prevention & control
D000276:Adjuvants, Immunologic; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin