Comparison of the effect of interleukin 6 and interleukin 1 on collagenase and proteoglycan production by chondrocytes.
In order to determine whether interleukin 6 (IL-6) is involved in the pathogenesis of the cartilage destruction observed in arthritis, the effect of human recombinant IL-6 on collagenase production and proteoglycan synthesis by bovine articular chondrocytes was examined. Addition of IL-6 (1.0 to 1000 U/ml) to the culture medium did not stimulate collagenase production or alter proteoglycan secretion. Whereas human purified interleukin 1 (IL-1) (20 U/ml) stimulated collagenase production and inhibited proteoglycan synthesis. Furthermore addition of IL-1 (20 U/ml) to chondrocyte cultures did not stimulate the chondrocytes to produce IL-6. Under our experimental conditions, IL-6 did not stimulate chondrocytes to proliferate as measured by [3H] thymidine incorporation. This would suggest that IL-6 is not involved in mediating cartilage loss.
Kandel
R A
RA
Department of Pathology, Mt. Sinai Hospital, Toronto, ON, Canada.
Petelycky
M
M
Dinarello
C A
CA
Minden
M
M
Pritzker
K P
KP
Cruz
T F
TF
D000818:Animals; D002356:Cartilage / Q000166:cytology* / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D007375:Interleukin-1 / Q000494:pharmacology* / Q000502:physiology; D015850:Interleukin-6 / Q000494:pharmacology* / Q000502:physiology; D003012:Microbial Collagenase / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D007375:Interleukin-1; D015850:Interleukin-6; D011509:Proteoglycans; D014316:Tritium; D003012:Microbial Collagenase; D013936:Thymidine