Activated macrophages in experimental subretinal neovascularization.
Laser-induced subretinal neovascularization (SRN) in monkey retinas was investigated by immunohistochemical techniques to identify the presence and location of activated macrophages. Retinal lesions were examined 3, 8 and 14 days after intensive argon laser treatment, and the distribution of interleukin-1 (IL-1)-containing cells in the lesions was determined by the orthogonal reconstruction of serial sections. Macrophages were present in the subretinal space of day 3 and day 8 lesions. These IL-1-containing cells were distributed about the area of rupture of Bruch's membrane and were quite common in lesions taken 3 days following laser treatment. While still apparent, the number was decreased at 8 days, and none were found 14 days after laser treatment. The temporal and spatial distribution of IL-1-staining macrophages paralleled the development of SRN, suggesting a relationship between the presence of activated macrophages and the initiation of neovascularization in this model.
Nishimura
T
T
Department of Ophthalmology, University of Southern California, Los Angeles.
Goodnight
R
R
Prendergast
R A
RA
Ryan
S J
SJ
D000818:Animals; D004195:Disease Models, Animal; D005451:Fluorescein Angiography; D005654:Fundus Oculi; D006801:Humans; D007124:Immunoenzyme Techniques; D007375:Interleukin-1 / Q000096:biosynthesis; D008028:Light Coagulation; D008252:Macaca fascicularis; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D015861:Retinal Neovascularization / Q000209:etiology* / Q000276:immunology
D007375:Interleukin-1