A comparison of monocyte oxidative responses in leprosy patients and healthy subjects as influenced by mycobacterial lipid pretreatment.
Superoxide anion (O2-) release by monocytes from leprosy patients in a paired study was lower than that released by monocytes from healthy controls. Pretreatment of healthy control monocytes with phenolic glycolipid-I (PGL-I) of Mycobacterium leprae resulted in the release of less O2- than released by buffer-treated cells or cells pretreated with structurally similar lipids. However, pretreatment of patient monocytes with PGL-I did not affect the O2- generation, perhaps because the cells already had a lower capacity to produce O2-. Upon further examination of the data from the patient population, monocytes from lepromatous patients released significantly less O2- than cells from normal controls, while tuberculoid patient cells released O2- in amounts similar to that generated by cells from normal controls. In addition, monocytes from patients with a high bacterial index had a lower capacity to generate O2- when compared to cells from healthy individuals.
Vachula
M
M
Department of Medicine, University of Illinois, Chicago.
Worobec
S
S
Andersen
B R
BR
D000328:Adult; D000942:Antigens, Bacterial / Q000276:immunology; D002478:Cells, Cultured; D005260:Female; D006017:Glycolipids / Q000276:immunology*; D006801:Humans; D007918:Leprosy / Q000378:metabolism*; D008055:Lipids / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D009166:Mycobacterium leprae / Q000276:immunology*; D010084:Oxidation-Reduction; D013481:Superoxides / Q000378:metabolism*
D000942:Antigens, Bacterial; D006017:Glycolipids; D008055:Lipids; C045169:phenolic glycolipid I, Mycobacterium leprae; C051618:mycoside A; D013481:Superoxides; C008901:phthiocerol dimycocerosate