Odorant-sensitive phospholipase C in insect antennae.
Exogenous tritiated phosphatidylinositol bisphosphate added to antennal preparations from locust and cockroach was hydrolysed releasing inositol trisphosphate. High activity of phospholipase C was detected in the soluble as well as in the membrane fraction. At low free calcium concentrations hydrolysis of the labelled lipid was stimulated by odorants and pheromones in a GTP-dependent manner. Consequently the level of inositol trisphosphate in antennal preparations increased upon odorant stimulation.
Boekhoff
I
I
Institute of Zoophysiology, University Stuttgart-Hohenheim, F.R.G.
Strotmann
J
J
Raming
K
K
Tareilus
E
E
Breer
H
H
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D003058:Cockroaches / Q000201:enzymology*; D003600:Cytosol / Q000201:enzymology; D004075:Diglycerides / Q000096:biosynthesis; D005260:Female; D006110:Grasshoppers / Q000201:enzymology*; D006160:Guanosine Triphosphate / Q000502:physiology; D015544:Inositol 1,4,5-Trisphosphate / Q000096:biosynthesis; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D009812:Odorants; D010675:Pheromones / Q000494:pharmacology*; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols / Q000378:metabolism*; D015290:Second Messenger Systems; D012679:Sense Organs / Q000201:enzymology*; D013045:Species Specificity; D010738:Type C Phospholipases / Q000378:metabolism*
D004075:Diglycerides; D008565:Membrane Proteins; D010675:Pheromones; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols; D015544:Inositol 1,4,5-Trisphosphate; D006160:Guanosine Triphosphate; D010738:Type C Phospholipases; D002118:Calcium