title: A guanine nucleotide-sensitive, glucagon-stimulated adenylyl cyclase in Candida albicans: effect of glucagon on cell morphology.
abstract: GTP, GTP-gamma-S and Gpp(NH)p produced a significant activation of Mg2(+)-dependent adenylyl cyclase in permeabilized cells of Candida albicans. This activation was inhibited by GDP-beta-S. Maximal stimulation (4-6 fold) was obtained when Mg2+ and GTP-gamma-S were added during permeabilization. The guanine nucleotide-stimulated activity could be further activated by glucagon in a dose dependent manner being the maximal stimulation (4-5 fold) attained at 10(-6) M glucagon. Addition of the hormone to yeast cells incubated under conditions conducive to produce germ tubes blocked hyphal development and promoted multibudded cell morphology.
authors.affiliation: Departamento de Química Biológica y Biología, Facultad de Ciencias Exactas y Naturales (Universidad de Buenos Aires), Argentina.
authors.forename: C
authors.initials: C
authors.lastname: Paveto
authors.forename: G
authors.initials: G
authors.lastname: Egidy
authors.forename: M A
authors.initials: MA
authors.lastname: Galvagno
authors.forename: S
authors.initials: S
authors.lastname: Passeron
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D002176:Candida albicans / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D004789:Enzyme Activation; D005934:Glucagon / Q000494:pharmacology*; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D013873:Thionucleotides / Q000494:pharmacology*
chemical_list: D013873:Thionucleotides; D006165:Guanylyl Imidodiphosphate; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate; D005934:Glucagon; D000262:Adenylyl Cyclases; D008274:Magnesium