Resistance of tumor-derived DNA to restriction enzyme digestion.
The major finding of this work is that there are specific restriction enzyme inhibitors present in "purified" tumor DNA which cause partial digestion patterns when tumor DNA is digested by standard procedures with any of three commonly employed restriction enzymes (HindIII, KpnI, XbaI). These aberrant patterns are not seen when DNA of cell lines derived from these tumors is digested. Thus, when working with tumor DNA these restriction enzymes should be used with caution.
Parkes
J L
JL
Institute of Environmental Medicine, New York University Medical Center, New York 10016.
Hubbard
F C
FC
Penn
A
A
D000818:Animals; D002461:Cell Line, Transformed; D003327:Coronary Disease / Q000235:genetics; D004262:DNA Restriction Enzymes / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000378:metabolism*; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D001749:Urinary Bladder Neoplasms / Q000235:genetics
D004273:DNA, Neoplasm; D004262:DNA Restriction Enzymes