Identification and characterization of autoantibodies against the nuclear envelope lamin B receptor from patients with primary biliary cirrhosis.
We have identified autoantibodies from two patients with primary biliary cirrhosis (PBC) that recognize the nuclear envelope of mammalian cells on indirect immunofluorescence microscopy. These antibodies bind to a 58-kD integral membrane protein (p58) of the turkey erythrocyte nuclear envelope, which has been previously identified as a membrane receptor for lamin B (Worman, H. J., J. Yuan, G. Blobel, and S. D. Georgatos. 1988. Proc. Natl. Acad. Sci. USA. 85:8531). The antibodies also bind to a 61-kD integral membrane protein (p61) of the rat liver nuclear envelope. Affinity-purified antibodies eluted from turkey p58 bind to rat p61, showing that the two proteins share an epitope(s) and that p61 is likely the rat liver lamin B receptor. In human nuclear envelopes, the antigen recognized has an apparent molecular mass close to that of avian protein. These findings, along with the previous discovery of autoantibodies against an integral membrane glycoprotein (gp210) of the nuclear pore membrane in patients with PBC, suggest that antibodies against integral membrane proteins of the nuclear envelope are characteristic of a subset of patients with PBC.
Courvalin
J C
JC
Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New Yor, New York 10021.
Lassoued
K
K
Worman
H J
HJ
Blobel
G
G
D000818:Animals; D001323:Autoantibodies / Q000276:immunology / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D034921:Lamin Type B; D034882:Lamins; D008105:Liver Cirrhosis, Biliary / Q000276:immunology*; D008970:Molecular Weight; D009685:Nuclear Envelope / Q000276:immunology*; D009687:Nuclear Proteins / Q000276:immunology*; D051381:Rats; D011956:Receptors, Cell Surface / Q000276:immunology*; D018160:Receptors, Cytoplasmic and Nuclear; D000097682:Lamin B Receptor
D001323:Autoantibodies; D034921:Lamin Type B; D034882:Lamins; D009687:Nuclear Proteins; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D000097682:Lamin B Receptor