Long insertions within telomeres contribute to chromosome size polymorphism in Plasmodium berghei.
During prolonged in vivo mitotic multiplication of a Plasmodium berghei ANKA clone (8417HP), parasites that contained an enlarged version of chromosome 4 were observed. Restriction mapping and hybridization results demonstrated that the extra DNA present in the enlarged chromosome consists of 2.3-kb tandem repeats, known to be normally located in subtelomeric position at several chromosomal ends but absent in the original chromosome. The inserted 2.3-kb units appeared to interrupt one of the original telomeres and to create an internal (approximately 1-kb-long) telomeric sequence.
Pace
T
T
Laboratory of Cellular Biology, Istituto Superiore di Sanità, Rome, Italy.
Ponzi
M
M
Dore
E
E
Janse
C
C
Mons
B
B
Frontali
C
C
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010962:Plasmodium berghei / Q000235:genetics*; D011110:Polymorphism, Genetic; D015183:Restriction Mapping
D004251:DNA Transposable Elements; D015345:Oligonucleotide Probes