title: Enhancement of interleukin-1 activity by murine cytomegalovirus infection of a macrophage cell line.
abstract: The effect of murine cytomegalovirus (MCMV) infection on interleukin 1 (IL-1) secretion was assessed using the macrophage cell lines P388D1 and J774A.1. The former proved to be nonpermissive for MCMV in that infectious virus and viral immediate early protein (pp89) were not expressed in these cells. MCMV infection of the P388D1 cells had no effect on release of biologically active IL-1. In contrast, J774A.1 cells, which were semipermissive for virus replication and pp89 expression, secreted enhanced levels of IL-1 activity following infection. The enhancement was evident when infection either preceded or followed lipopolysaccharide stimulation of the macrophages. The relative proportion of IL-1 alpha and beta secreted from MCMV-infected cells was similar to noninfected controls. In addition, the bioactivity of intracellular IL-1 alpha escaping membranes of fixed cells was unaffected by virus infection. From these findings, we conclude that limited MCMV expression in the J774A.1 macrophage cell line enhances secretion of IL-1 alpha and beta bioactivity.
authors.affiliation: Department of Microbiology and Immunology, Eastern Virginia Medical School, Norfolk, Virginia 23501.
authors.forename: M A
authors.initials: MA
authors.lastname: Lewis
authors.forename: J S
authors.initials: JS
authors.lastname: Slater
authors.forename: L I
authors.initials: LI
authors.lastname: Leverone
authors.forename: A E
authors.initials: AE
authors.lastname: Campbell
mesh_terms: D000818:Animals; D002460:Cell Line; D003587:Cytomegalovirus; D005260:Female; D007375:Interleukin-1 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011247:Pregnancy; D014775:Virus Activation
chemical_list: D007375:Interleukin-1; D008070:Lipopolysaccharides