Low-resolution structure of the tetrameric phenylalanyl-tRNA synthetase from Escherichia coli. A neutron small-angle scattering study of hybrids composed of protonated and deuterated protomers.
Escherichia coli phenylalanyl-tRNA synthetase is a tetrameric protein composed of two types of protomers. In order to resolve the subunit organization, neutron small-angle scattering experiments have been performed in different contrasts with all types of isotope hybrids that could be obtained by reconstituting the alpha 2 beta 2 enzyme from the protonated and deuterated forms of the alpha and beta subunits. Experiments have been also made with the isolated alpha promoter. A model for the alpha 2 beta 2 tetramer is deduced where the two alpha promoters are elongated ellipsoids (45 x 45 x 160 A3) lying side by side with an angle of about 40 degrees between their long axes and where the two beta subunits are also elongated ellipsoids (31 x 31 x 130 A3) with an angle of 30 degrees between their axes. This model was obtained by assuming that the two pairs of subunits are in contact in an orthogonal manner and by taking advantage of the measured distance between the centers of mass of the alpha 2 and beta 2 pairs (d = 23 +/- 2 A).
Dessen
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Laboratoire de Biochimie, Ecole Polytechnique, Palaiseau, France.
Ducruix
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A
May
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Blanquet
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D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification; D003903:Deuterium; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development; D046911:Macromolecular Substances; D008433:Mathematics; D008961:Models, Structural; D008962:Models, Theoretical; D009502:Neutrons; D010652:Phenylalanine-tRNA Ligase / Q000302:isolation & purification; D011487:Protein Conformation; D055503:Protein Multimerization; D011522:Protons; D012542:Scattering, Radiation
D046911:Macromolecular Substances; D011522:Protons; D003903:Deuterium; D000604:Amino Acyl-tRNA Synthetases; D010652:Phenylalanine-tRNA Ligase