Differential production of IFN-alpha/beta by CSF-1- and GM-CSF-derived macrophages.
Mature macrophages, derived in vitro from bone marrow progenitors under the influence of either macrophage colony stimulating factor (CSF-1) or granulocyte-macrophage (GM)-CSF, have been shown to differ morphologically and functionally. The data presented in this report demonstrate that macrophages derived from bone marrow progenitors under the influence of CSF-1 are highly resistant to infection with vesicular stomatitis virus (VSV), and that this refractoriness can be reversed by treatment of cells with anti-IFN-alpha/beta antibody. In contrast, macrophages derived from bone marrow progenitors under the influence of GM-CSF are highly susceptible to the cytopathic effects of VSV, but can be protected by very low concentrations of exogenous IFN-alpha/beta. These findings suggest that CSF-1 derived macrophages have a greater capacity for the production and/or utilization of IFN-alpha/beta than GM-CSF-derived macrophages, which may account for many of the differentiative differences reported previously.
Falk
L A
LA
Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814.
Vogel
S N
SN
D000818:Animals; D001853:Bone Marrow / Q000382:microbiology; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D005960:Glucosides; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000494:pharmacology*; D007370:Interferon Type I / Q000378:metabolism*; D016173:Macrophage Colony-Stimulating Factor; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology; D051379:Mice; D014721:Vesicular stomatitis Indiana virus / Q000502:physiology; D014779:Virus Replication / Q000187:drug effects / Q000502:physiology
D003115:Colony-Stimulating Factors; D005960:Glucosides; D006133:Growth Substances; D007370:Interferon Type I; C018619:octyl-beta-D-glucoside; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor