Identification of ultraviolet-inducible proteins that bind to a TGACAACA sequence in the polyoma virus regulatory region.
The exposure of mammalian cells to UV light or other DNA-damaging agents induces several responses which may provide cellular defense mechanisms and also play a role in carcinogenesis. Employing a 257-base pair DNA fragment from the polyoma virus that contains the origin of replication and regulatory region of this virus, we have identified a set of DNA-binding proteins that are induced in normal rat fibroblasts at 6-24 h after UV exposure. These proteins bind to a specific octamer sequence (TGACAACA) designated the "UV response element." Purification of these inducible proteins on a UV response element affinity column revealed a set of proteins, among which the major protein has a molecular weight of 40,000, which co-purify with c-fos but do not react with antibodies to c-jun-encoded proteins. These UV-induced proteins may, in concert with other cellular components, play a role in mediating specific cellular responses to DNA damage in mammalian cells.
Ronai
Z A
ZA
Comprehensive Cancer Center, Columbia University, New York, New York 10032.
Weinstein
I B
IB
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism* / Q000528:radiation effects; D005809:Genes, Regulator; D005814:Genes, Viral; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015345:Oligonucleotide Probes; D010957:Plasmids; D011120:Polyomavirus / Q000235:genetics*; D051381:Rats; D015183:Restriction Mapping; D014466:Ultraviolet Rays
D004268:DNA-Binding Proteins; D015345:Oligonucleotide Probes