Enzymes of phosphoinositide synthesis in secretory vesicles destined for the plasma membrane in Saccharomyces cerevisiae.
CDP-diacylglycerol synthase, phosphatidylinositol synthase, and phosphatidylinositol kinase activities were associated with post-Golgi apparatus secretory vesicles destined for the plasma membrane of Saccharomyces cerevisiae. These results suggest that the plasma membrane is capable of synthesizing both CDP-diacylglycerol and phosphatidylinositol as well as phosphorylating phosphatidylinositol.
Kinney
A J
AJ
Department of Food Science, Cook College, Rutgers University, New Brunswick, New Jersey 08903.
Carman
G M
GM
D019870:1-Phosphatidylinositol 4-Kinase; D051607:CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase; D002462:Cell Membrane / Q000201:enzymology; D003594:Cytoplasmic Granules / Q000201:enzymology*; D046911:Macromolecular Substances; D008861:Microsomes / Q000201:enzymology; D008970:Molecular Weight; D009713:Nucleotidyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000096:biosynthesis*; D010770:Phosphotransferases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins; D017855:Transferases (Other Substituted Phosphate Groups)
D046911:Macromolecular Substances; D010716:Phosphatidylinositols; D029701:Saccharomyces cerevisiae Proteins; D010770:Phosphotransferases; D019870:1-Phosphatidylinositol 4-Kinase; C441760:PIK1 protein, S cerevisiae; D009713:Nucleotidyltransferases; C022348:phosphatidate cytidylyltransferase; D017855:Transferases (Other Substituted Phosphate Groups); D051607:CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase