Regulation of the Na(+)-K+ pump activity and estimation of the reserve capacity in intact rat peritoneal mast cells.
Evidence is provided that regulation of the Na(+)-K+ pump activity in rat peritoneal mast cells occurs mainly through stimulation of the pump from inside the plasma membrane by sodium. It is demonstrated that there is a large reserve capacity for the exchange of intracellular sodium with extracellular potassium in these cells. The maximal pump activity was estimated to be 3230 pmol/10(6) cells per min and Km for extracellular potassium was 1.5 mM.
Knudsen
T
T
Department of Pharmacology, Odense University, Denmark.
Johansen
T
T
D000818:Animals; D001693:Biological Transport, Active; D002469:Cell Separation; D066298:In Vitro Techniques; D008297:Male; D008407:Mast Cells / Q000201:enzymology*; D010042:Ouabain / Q000494:pharmacology; D010529:Peritoneal Cavity / Q000166:cytology; D011188:Potassium / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012413:Rubidium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism*
D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase; D012413:Rubidium; D011188:Potassium