Simian virus 40 T antigen can transcriptionally activate and mediate viral DNA replication in cells which lack the retinoblastoma susceptibility gene product.
Simian virus 40 T antigen is a multifunctional protein which has recently been shown to form a complex with the retinoblastoma susceptibility gene product (Rb protein) (J.A. DeCaprio, J.W. Ludlow, J. Figge, J.-Y. Shaw, C.-M. Huang, W.-H. Lee, E. Marsilio, E. Paucha, and D.M. Livingston, Cell 54:275-283, 1988; P. Whyte, K.J. Buchkovich, J.M. Horowitz, S.H. Friend, M. Raybuck, R.A. Weinberg, and E. Harlow, Nature (London) 334:124-129, 1988). This interaction may facilitate some of the functions of T antigen. The ability of simian virus 40 T antigen to mediate transcriptional activation and viral DNA replication was tested in human osteosarcoma cell lines U-2OS and Saos-2, which are Rb positive and Rb negative, respectively. Both functions of T antigen were efficient in both cell lines. Hence, these functions can occur in the absence of Rb protein.
Trifillis
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P
Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia 19104-6076.
Picardi
J
J
Alwine
J C
JC
D000952:Antigens, Polyomavirus Transforming; D002460:Cell Line; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D004198:Disease Susceptibility; D006801:Humans; D012516:Osteosarcoma; D010750:Phosphoproteins / Q000172:deficiency / Q000235:genetics*; D011401:Promoter Regions, Genetic; D016160:Retinoblastoma Protein; D013539:Simian virus 40 / Q000235:genetics* / Q000276:immunology; D015533:Transcriptional Activation
D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D010750:Phosphoproteins; D016160:Retinoblastoma Protein