Different pathways of endothelin/sarafotoxin-stimulated phosphoinositide hydrolysis in myocytes.
Aging of rat heart myocytes in culture is accompanied by approximately 50% reduction in endothelin (ET)/sarafotoxin (SRTX) receptor-binding capacity as well as in the induction of phosphoinositide (PI) hydrolysis. Treatment of aged cultures under conditions yielding myocytes with a lipid composition similar to that in young cultures restored all the ET/SRTX receptors; at the same time it re-established only the endothelin-induced but not the sarafotoxin-induced PI-hydrolysis response. Thus more than one mechanism may stimulate PI metabolism.
Galron
R
R
Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.
Kloog
Y
Y
Bdolah
A
A
Sokolovsky
M
M
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D016232:Endothelins; D007457:Iodine Radioisotopes; D008081:Liposomes / Q000378:metabolism; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology*; D010713:Phosphatidylcholines / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000378:metabolism; D017466:Receptors, Endothelin; D018000:Receptors, Peptide; D014757:Viper Venoms / Q000494:pharmacology*
D016232:Endothelins; D007457:Iodine Radioisotopes; D008081:Liposomes; D010455:Peptides; D010713:Phosphatidylcholines; D010716:Phosphatidylinositols; D011956:Receptors, Cell Surface; D011950:Receptors, Cholinergic; D017466:Receptors, Endothelin; D018000:Receptors, Peptide; D014757:Viper Venoms; C058085:sarafotoxin receptor; C057354:sarafotoxins s6