New method for titration of virus infectivity by immunostaining.
We have developed a new method for titration of viruses utilizing automated microtiter technology. Compared to existing methods such as plaque assay or hemagglutination titration of influenza virus, the new method offers distinct advantages in terms of time and effort. In addition because multiple replicates can easily be employed accuracy can be increased.
Usuba
O
O
Department of Microbiology, Mount Sinai School of Medicine, New York, NY.
Schulman
J L
JL
Deatly
A M
AM
Bona
C A
CA
Moran
T M
TM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D002460:Cell Line; D005069:Evaluation Studies as Topic; D006387:Hemagglutination, Viral; D007124:Immunoenzyme Techniques; D009980:Influenza A virus / Q000472:pathogenicity; D051379:Mice; D008807:Mice, Inbred BALB C; D012680:Sensitivity and Specificity; D018139:Simplexvirus / Q000472:pathogenicity; D014709:Vero Cells; D010948:Viral Plaque Assay; D014773:Virology / Q000379:methods*; D014780:Viruses / Q000472:pathogenicity*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral