Computer simulation in template-directed oligonucleotide synthesis.
A computer simulation (KINSIM) modeling up to 33 competing reactions was used in order to investigate the product distribution in a template-directed oligonucleotide synthesis as a function of time and concentration of the reactants. The study is focused on the poly(C)-directed elongation reaction of an oligoguanylate (a 7-mer is chosen) with guanosine 5'-monophosphate-2-methyl-imidazolide (2-MeImpG), the activated monomer. It is known that the elongation of oligoguanylates to form oligomeric products such as 8-mer, 9-mer, 10-mer, etc., is in competition with (1) the dimerization and further oligomerization reaction of 2-MeImpG that leads to the formation of dimers and short oligomers, and (2) the hydrolysis of 2-MeImpG that forms inactive guanosine 5'-monophosphate, 5'-GMP. Experimentally determined rate constants for the above three processes at 37 degrees C and pH 7.95 were used in the simulation; the initial concentrations of 2-MeImpG, [M]o, and of the oligoguanylate primer, [7-mer]o, were varied, and KINSIM calculated the distribution of products as a function of time until equilibration was reached, i.e., when all the activated monomer has been consumed. In order to sort out how strongly the elongation reaction may be affected by the competing hydrolysis and dimerization, we also simulated the idealized situation in which these competing reactions do not occur.(ABSTRACT TRUNCATED AT 250 WORDS)
Kanavarioti
A
A
Chemistry Department, University of California, Santa Cruz 95064.
Bernasconi
C F
CF
D001704:Biopolymers; D003198:Computer Simulation; D006157:Guanosine Monophosphate / Q000031:analogs & derivatives* / Q000737:chemistry; D006868:Hydrolysis; D007700:Kinetics; D009841:Oligonucleotides / Q000138:chemical synthesis*; D011066:Poly C / Q000737:chemistry; D013698:Templates, Genetic
D001704:Biopolymers; D009841:Oligonucleotides; D011066:Poly C; C036896:guanosine 5'-phospho-2-methylimidazolide; D006157:Guanosine Monophosphate