Comparison of performances of four ELISA kits in the detection of BLV antibodies in bulk tank milk or concentrated lactoserum from herds with low prevalence of infection.
Performances of four ELISA kits in the detection of BLV antibodies in bulk tank milk was studied in 76 non-infected herds and 44 herds with low prevalence of BLV infection. None of the kits gave false positive results. On the other hand, there was an important variation in sensitivity. The kits with the highest sensitivity identified 43% of infected herds, which included 65% of infected cows. When concentrated lactoserum was tested, 59% of infected herds, which included 73% of infected cows, could be identified.
Eloit
M
M
Ecole Vétérinaire, Maisons-Alfort, France.
Kuzmak
J
J
Dheiller
M
M
Bénet
J J
JJ
Toma
B
B
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007938:Leukemia / Q000453:epidemiology / Q000662:veterinary; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D008892:Milk / Q000276:immunology*; D011247:Pregnancy; D012190:Retroviridae / Q000276:immunology*; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral