title: Labeling of the neurons of origin of zinc-containing pathways by intraperitoneal injections of sodium selenite.
abstract: Intraperitoneal injections of sodium selenite result in the formation of zinc-selenium complexes in zinc-containing axonal boutons ("Timm stainable boutons"), and the zinc-selenium precipitate can be rendered visible in histological sections by silver enhancement. In this work we present evidence, in the rat, that zinc-selenium precipitates formed in vivo after intraperitoneal injections of sodium selenite are translocated by colchicine-sensitive retrograde transport to neural perikarya when animals are allowed to survive 12-24 h after the selenite administration. Silver enhancement renders the perikaryal precipitates visible and thus demonstrates the perikarya of all zinc-containing neurons in the CNS simultaneously. Large populations of zinc-containing neurons identified by the method are found in layers II, III, and VI of all neocortical areas, in the superficial and deep layers of the prepyriform areas and, with a high degree of regional differentiation, in the retrosplenial, entorhinal, para- and presubicular cortices, the hippocampal formation and the amygdaloid complex. Zinc-containing cells were absent from the caudate-putamen, nucleus accumbens and septal complex. Labeled zinc-containing cells are absent in non-telencephalic parts of the brain. The findings indicate that the zinc-containing circuitry of the brain mainly serves in telencephalic information processing.
authors.affiliation: Department of Neurobiology, University of Aarhus, Denmark.
authors.forename: L
authors.initials: L
authors.lastname: Slomianka
authors.forename: G
authors.initials: G
authors.lastname: Danscher
authors.forename: C J
authors.initials: CJ
authors.lastname: Frederickson
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002470:Cell Survival; D003078:Colchicine / Q000494:pharmacology; D004351:Drug Resistance; D005260:Female; D007274:Injections, Intraperitoneal; D008297:Male; D009434:Neural Pathways / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011921:Rats, Inbred WKY; D012643:Selenium / Q000506:poisoning; D018038:Sodium Selenite; D013045:Species Specificity; D013997:Time Factors; D015032:Zinc / Q000378:metabolism*
chemical_list: D012643:Selenium; D018038:Sodium Selenite; D015032:Zinc; D003078:Colchicine