title: Rapid dysplastic transformation of human genital cells by human papillomavirus type 18.
abstract: This study delineates differences in biologic activity between human papillomavirus (HPV) types 16 and 18. Human cervical and foreskin epithelial cells were cultured and transfected with recombinant HPV-16 and -18 DNA, resulting in immortalized cell lines. Normal epithelial cells as well as HPV-16 and -18 immortalized cells of both early passages (less than 40 population doublings) and late passages (greater than 180 population doublings) were transplanted in athymic mice. Normal squamous cells formed well-stratified epithelium, while HPV immortalized cells developed either normal-appearing epithelium or typical dysplastic changes. Dysplastic changes were seen in none of the 13 grafts with early-passage HPV-16 cell lines, while 9 of 14 grafts with early-passage HPV-18 cell lines developed dysplasias (P less than 0.0004). These results support previous clinical observations suggesting that HPV-18 may be associated with a more aggressive and rapidly progressive form of cervical neoplasia.
authors.affiliation: Department of Obstetrics and Gynecology, Georgetown University Medical Center, Washington, DC 20007.
authors.forename: W
authors.initials: W
authors.lastname: Barnes
authors.forename: G
authors.initials: G
authors.lastname: Woodworth
authors.forename: S
authors.initials: S
authors.lastname: Waggoner
authors.forename: M
authors.initials: M
authors.lastname: Stoler
authors.forename: A B
authors.initials: AB
authors.lastname: Jenson
authors.forename: G
authors.initials: G
authors.lastname: Delgado
authors.forename: J
authors.initials: J
authors.lastname: DiPaolo
mesh_terms: D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002584:Cervix Uteri / Q000166:cytology*; D004274:DNA, Recombinant; D004847:Epithelial Cells; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D027383:Papillomaviridae / Q000472:pathogenicity*; D010413:Penis / Q000166:cytology*; D014162:Transfection; D014183:Transplantation, Heterologous
chemical_list: D004274:DNA, Recombinant