Lithium hydroxide/aqueous methanol: mild reagent for the hydrolysis of bile acid methyl esters.
An efficient and convenient procedure for the hydrolysis of bile acid methyl esters is described. This is achieved by the addition of aqueous lithium hydroxide in methanol/dioxane (or tetrahydrofuran) at room temperature. Under these conditions, the formates as well as the acetate derivatives were also hydrolyzed, and the desired bile acids were isolated in 86 to 94% yield.
Dayal
B
B
Department of Medicine, UMDNJ-New Jersey Medical School, Newark.
Salen
G
G
Toome
B
B
Tint
G S
GS
Shefer
S
S
Padia
J
J
D001647:Bile Acids and Salts; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D002942:Circular Dichroism; D004952:Esters; D006868:Hydrolysis; D007202:Indicators and Reagents; D008094:Lithium; D018020:Lithium Compounds; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D000432:Methanol; D011522:Protons
D001647:Bile Acids and Salts; D004952:Esters; D007202:Indicators and Reagents; D018020:Lithium Compounds; D011522:Protons; D008094:Lithium; C028467:lithium hydroxide; D000432:Methanol