title: Ubiquitin in Chlamydomonas reinhardii. Distribution in the cell and effect of heat shock and photoinhibition on its conjugate pattern.
abstract: Ubiquitin, a highly conserved 76-amino-acid protein, is involved in the response of many types of eukaryotic cells to stress but little is known about its role in lower plants. In the present study we have investigated the distribution of ubiquitin in the unicellular alga Chlamydomonas reinhardii as well as the effect of heat and light stress on its conjugation to cellular proteins. Immunoelectron microscopy shows that ubiquitin is located in the chloroplast, nucleus, cytoplasm, pyrenoid and on the plasma membrane. The location of ubiquitin within chloroplasts has not been observed previously. In immunoblots of whole cell extracts with an antibody to ubiquitin a prominent conjugate band with an apparent molecular mass of 29 kDa and a broad region of high-molecular-mass conjugates (apparent molecular mass greater than 45 kDa) were observed. Exposure of cells to a 41.5 degrees C heat shock in both the dark and light caused the disappearance of the 29-kDa conjugate and an increase in the high-molecular-mass conjugates. After step down to 25 degrees C the 29-kDa conjugate reappeared while the levels of high-molecular-mass conjugates decreased. In light, the recovery of the 29-kDa band was more rapid than in the dark. Photoinhibition alters the ubiquitin conjugation pattern similarly to heat shock, but to a lesser degree. These observations imply that, in Chlamydomonas, ubiquitin has a role in the chloroplast and in the response to heat and light stress.
authors.affiliation: Botanisches Institut, Technischen Universität Braunschweig, Federal Republic of Germany.
authors.forename: M
authors.initials: M
authors.lastname: Wettern
authors.forename: H A
authors.initials: HA
authors.lastname: Parag
authors.forename: L
authors.initials: L
authors.lastname: Pollmann
authors.forename: I
authors.initials: I
authors.lastname: Ohad
authors.forename: R G
authors.initials: RG
authors.lastname: Kulka
mesh_terms: D002462:Cell Membrane / Q000032:analysis; D002467:Cell Nucleus / Q000032:analysis; D002696:Chlamydomonas / Q000032:analysis* / Q000648:ultrastructure; D002736:Chloroplasts / Q000032:analysis; D003593:Cytoplasm / Q000032:analysis; D006358:Hot Temperature; D015151:Immunoblotting; D007150:Immunohistochemistry; D008027:Light; D008854:Microscopy, Electron; D008970:Molecular Weight; D011506:Proteins / Q000378:metabolism; D014452:Ubiquitins / Q000032:analysis* / Q000378:metabolism
chemical_list: D011506:Proteins; D014452:Ubiquitins