In situ detection of enterovirus genomes in mouse myocardial tissue by ribonucleic acid probes.
We have applied a sensitive in situ hybridization method for the detection of coxsackievirus RNA in myocardial tissue. Two radioactive cRNA probes were used: an RNA transcript representing the 5' end of poliovirus 3 which recognizes a highly conserved region among enteroviruses and an RNA transcript of a 1.1 kb fragment from the polymerase gene region of coxsackievirus B3. The reactivity of these probes was tested by dot-blot hybridization against a panel of enteroviruses. Formaldehyde-fixed and paraffin-embedded tissue of experimentally coxsackievirus B3 infected mice was analyzed for the localization of virus RNA. Both the probes gave signals in mouse myocardial cells, disseminated evenly in the heart tissue 7 days postinfection. At this time point, hybridization-positive cells and inflammatory reaction were mainly found in different areas that may be due to the inability of the immune system to recognize the infected cells before cytolysis. The samples were still positive when fixed 52 hours postmortem indicating that virus RNA is in a relatively stable form in the cells.
Kallajoki
M
M
Department of Pathology, University of Turku, Finland.
Kalimo
H
H
Wesslén
L
L
Auvinen
P
P
Hyypiä
T
T
D000818:Animals; D003384:Coxsackievirus Infections / Q000175:diagnosis / Q000473:pathology; D004770:Enterovirus / Q000235:genetics* / Q000302:isolation & purification; D004769:Enterovirus Infections / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D006321:Heart / Q000382:microbiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011051:Poliomyelitis / Q000175:diagnosis / Q000473:pathology; D017955:Poliovirus / Q000235:genetics / Q000302:isolation & purification; D015347:RNA Probes; D012367:RNA, Viral / Q000032:analysis*
D015347:RNA Probes; D012367:RNA, Viral