Fusion of Rous-sarcoma-virus-transformed rat cells to morphologically normal human or rat cells results in transcriptional suppression of the provirus that depends on its chromosomal integration site.
The fusion of a Rous sarcoma virus (RSV)-transformed rat fibroblast clone to at least 2 different human cell types reproducibly produces phenotypically normal hybrids. Analysis of such hybrids reveals that proviral silence is the result of transcriptional down-regulation, presumably by a trans-acting human molecule. Furthermore, this phenomenon seems to be strongly influenced by the proviral chromosomal integration site and its imposition may entail a mechanism that is required only transiently.
Green
A R
AR
Imperial Cancer Research Fund Laboratories, St Bartholomew's Hospital, London, UK.
Poole
C J
CJ
Povey
S M
SM
Rowe
D
D
Searle
S
S
Wyke
J A
JA
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D002459:Cell Fusion; D002472:Cell Transformation, Viral / Q000235:genetics*; D002478:Cells, Cultured / Q000382:microbiology / Q000648:ultrastructure; D002877:Chromosomes, Human / Q000382:microbiology / Q000648:ultrastructure*; D015536:Down-Regulation / Q000235:genetics; D005347:Fibroblasts / Q000382:microbiology / Q000648:ultrastructure; D006801:Humans; D006822:Hybrid Cells / Q000382:microbiology / Q000648:ultrastructure; D010641:Phenotype; D011533:Proviruses / Q000235:genetics*; D051381:Rats; D014158:Transcription, Genetic / Q000235:genetics*