title: Monoclonal antibodies to endothelin: application for sandwich immunoassays.
abstract: We established 16 monoclonal antibodies (MAbs) to endothelin (ET), a novel and potent vasoconstrictor. They were classified into two groups: eight of them reacted with the C-terminal portion of ET, and the other eight reacted with the non-C-terminal portion. We constructed the sandwich enzyme linked immunosorbent assays (ELISAs) using a combination of a MAb belonging to the former with that belonging to the latter, and could detect about 400 pg/ml of ET.
authors.affiliation: Diagnostic Division, Yamasa Shoyu Co., Ltd., Chiba, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Hamaoki
authors.forename: H
authors.initials: H
authors.lastname: Kato
authors.forename: M
authors.initials: M
authors.lastname: Sugi
authors.forename: M
authors.initials: M
authors.lastname: Fujimoto
authors.forename: H
authors.initials: H
authors.lastname: Kurihara
authors.forename: M
authors.initials: M
authors.lastname: Yosizumi
authors.forename: M
authors.initials: M
authors.lastname: Yanagisawa
authors.forename: S
authors.initials: S
authors.lastname: Kimura
authors.forename: T
authors.initials: T
authors.lastname: Masaki
authors.forename: Y
authors.initials: Y
authors.lastname: Yazaki
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D016232:Endothelins; D004730:Endothelium, Vascular / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000276:immunology; D010455:Peptides / Q000032:analysis / Q000276:immunology*; D014662:Vasoconstrictor Agents / Q000032:analysis / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D016232:Endothelins; D010455:Peptides; D014662:Vasoconstrictor Agents