title: High incidence of urinary JC virus excretion in nonimmunosuppressed older patients.
abstract: Urine specimens from 120 patients attending urologic clinics were screened by blot hybridization for the presence of a polyomavirus DNA. Detected viral DNA was then identified as BK or JC by fine restriction enzyme analysis. BK and JC viral DNA was found in 5 (4%) and 35 (29%) patients, respectively. Detection rates were compared among three age groups: 0-29, 30-59, and 60-89 years. Detection of JC viral DNA increased with age and reached the highest value (45%) in the group aged 60-89 years. For BK viral DNA a correlation between detection and age was not clear because the rate of detection was low, although the highest rate (9%) occurred in the oldest group. To confirm the active urinary excretion of polyomavirus DNA in older patients, urine specimens from 23 patients (60-90 years) treated at an internal medicine clinic were examined for viral DNA. BK and JC viral DNA were in 2 (9%) and 12 patients (52%), respectively. These results suggest that JC virus is frequently reactivated in older individuals.
authors.affiliation: Department of Urology, Branch Hospital, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kitamura
authors.forename: Y
authors.initials: Y
authors.lastname: Aso
authors.forename: N
authors.initials: N
authors.lastname: Kuniyoshi
authors.forename: K
authors.initials: K
authors.lastname: Hara
authors.forename: Y
authors.initials: Y
authors.lastname: Yogo
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D001345:Autoradiography; D001739:BK Virus / Q000235:genetics / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D015342:DNA Probes; D004279:DNA, Viral / Q000652:urine*; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D015994:Incidence; D007223:Infant; D007577:JC Virus / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D011120:Polyomavirus / Q000302:isolation & purification*; D012008:Recurrence; D015183:Restriction Mapping; D014412:Tumor Virus Infections / Q000453:epidemiology* / Q000652:urine; D014556:Urine / Q000382:microbiology*
chemical_list: D015342:DNA Probes; D004279:DNA, Viral