Application of countercurrent chromatography/thermospray mass spectrometry for the identification of bioactive lignans from plant natural products.
The versatility and resolving power of countercurrent chromatography (CCC) has been demonstrated with a newly developed analytical high-speed planet centrifuge system. Interfacing countercurrent chromatography with mass spectrometry (MS) provides a new analytical methodology that integrates the advantages of countercurrent chromatography with the low detection limit and identification capability of mass spectrometry. In this paper the capability of thermospray CCC/MS is demonstrated in identifying and validating the bioactive and structurally known lignans from a crude extract of Schisandra rubriflora Rhed et Wils, a traditional Chinese herbal medicine for treatment of hepatitis.
Lee
Y W
YW
Research Triangle Institute, North Carolina 27709.
Voyksner
R D
RD
Pack
T W
TW
Cook
C E
CE
Fang
Q C
QC
Ito
Y
Y
D002845:Chromatography / Q000379:methods*; D017705:Lignans; D008031:Lignin / Q000032:analysis*; D013058:Mass Spectrometry; D010944:Plants / Q000032:analysis*
D017705:Lignans; D008031:Lignin