Removal of cryptic poxvirus transcription termination signals from the human immunodeficiency virus type 1 envelope gene enhances expression and immunogenicity of a recombinant vaccinia virus.
The in vivo role of the proposed poxvirus early transcription termination signal TTTTTNT was confirmed by analysis of the RNA species made by recombinant vaccinia viruses. Premature transcription termination occurred following each of two TTTTTNT sequences present naturally within the coding region of the human immunodeficiency virus type 1 envelope gene. Alteration of the TTTTTNT sequences, without changing the encoded amino acids, resulted in production of full-length early mRNAs, improved protein expression, and a more consistent immune response.
Earl
P L
PL
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
Hügin
A W
AW
Moss
B
B
D000818:Animals; D001483:Base Sequence; D015686:Gene Products, env / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D005809:Genes, Regulator; D015752:Genes, env; D015483:HIV Antibodies / Q000276:immunology; D015488:HIV Antigens / Q000032:analysis; D015497:HIV-1 / Q000235:genetics; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D011212:Poxviridae / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D011995:Recombination, Genetic; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic; D014616:Vaccinia virus / Q000235:genetics
D015686:Gene Products, env; D015483:HIV Antibodies; D015488:HIV Antigens; D015345:Oligonucleotide Probes; D012333:RNA, Messenger