Role of free radicals produced during the metabolism of mitomycin C in Escherichia coli inactivation.
1. The primary objective of this study was to assess whether reactive oxygen species produced during the metabolism of mitomycin C are responsible for, or contribute to, the induction of the cytotoxic response. 2. Escherichia coli wild-type and superoxide dismutase mutants were grown either anoxically or euoxically, in K medium or minimal glucose medium, and treated with mitomycin C in K medium or M9 salts. The cytotoxic response did not vary significantly between the wild-type and the SOD mutants. 3. The toxicity of mitomycin C was not affected by the hydroxyl radical scavengers, dimethyl sulphoxide or ethanol, whereas protection was afforded by thiourea. 4. It is concluded that oxygen radical species do not play a significant role in mediating mitomycin C inactivation of normally growing bacteria.
Schiavano
G F
GF
Istituto di Scienze Tossicologiche, Università degli Studi di Urbino, Italy.
Brandi
G
G
Salvaggio
L
L
Cattabeni
F C
FC
Cantoni
O
O
D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology; D005609:Free Radicals; D006878:Hydroxides / Q000378:metabolism; D017665:Hydroxyl Radical; D016685:Mitomycin; D008937:Mitomycins / Q000378:metabolism* / Q000494:pharmacology; D009154:Mutation; D010100:Oxygen / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000235:genetics; D013890:Thiourea / Q000494:pharmacology
D005609:Free Radicals; D006878:Hydroxides; D008937:Mitomycins; D017665:Hydroxyl Radical; D000431:Ethanol; D016685:Mitomycin; D013482:Superoxide Dismutase; D013890:Thiourea; D010100:Oxygen; D004121:Dimethyl Sulfoxide