Gene mapping of infectious bovine rhinotracheitis viral DNA genome.
A bovine herpesvirus I (BHV-I) HindIII genomic bank spanning 89% of the entire genome was constructed and individual fragments analyzed for their capacity to select specific mRNAs which were then expressed by in vitro translation assays. This procedure allowed the mapping of more than 20 viral polypeptides to discrete regions of the DNA genome. Some polypeptides map in neighboring HindIII fragments while most seem encoded in single fragments. In particular, the coding sequences for an abundant 94 kDa polypeptide, which is the potential unglycosylated precursor of gII glycoprotein, have been assigned to the small 3.6 kbp HindIII genomic fragment M. The localization of structural and non-structural gene-coding sequences will help to characterize viral polypeptides and eventually, a better understanding of BHV-I infection will be gained.
Simard
C
C
Institut Armand-Frappier, Université du Québec, Laval, Canada.
Nadon
F
F
Séguin
C
C
LaBoissière
S
S
Trudel
M
M
D002874:Chromosome Mapping; D004279:DNA, Viral / Q000235:genetics*; D015723:Gene Library; D005814:Genes, Viral; D005815:Genetic Code; D007242:Herpesvirus 1, Bovine / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010455:Peptides / Q000235:genetics; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping; D015678:Viral Structural Proteins / Q000235:genetics
D004279:DNA, Viral; D010455:Peptides; D012333:RNA, Messenger; D012367:RNA, Viral; D015678:Viral Structural Proteins