title: Inhibition of DNA synthesis and cytotoxic effects of some DNA topoisomerase II and gyrase inhibitors in Chinese hamster V79 cells.
abstract: In this study, some DNA topoisomerase II and gyrase inhibitors have been identified as inhibitors of polymerization of deoxyribonucleotides [novobiocin (NVB), nalidixic acid (NDA), oxolinic acid (OXA)], or inhibitors of replicon initiation and DNA-chain elongation [etoposide (VP-16), teniposide (VM-26), 4'-(9-acridinylamino)methansulfon-m-anisidine (m-AMSA), ellipticine (ELT)]. The inhibitors of deoxyribonucleotide polymerization produced a significant (greater than 85%) suppression of [3H]thymidine incorporation into V79 cells within 20 min of treatment, followed by a rapid recovery of DNA synthesis, and reduced cell killing. In contrast, the inhibitors of replicon initiation and DNA-chain elongation needed about 60 min to induce a partial, but irreversible inhibition of DNA replication, associated with extensive cell killing.
authors.affiliation: Department of Radiology and Radiation Biology, Colorado State University, Fort Collins 80523.
authors.forename: D
authors.initials: D
authors.lastname: Suciu
mesh_terms: D000818:Animals; D002458:Cell Fractionation; D002467:Cell Nucleus; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects* / Q000528:radiation effects; D003854:Deoxyribonucleotides / Q000378:metabolism; D012093:Replicon / Q000187:drug effects; D059005:Topoisomerase II Inhibitors
chemical_list: D003854:Deoxyribonucleotides; D059005:Topoisomerase II Inhibitors