Sequential initiation of lagging and leading strand synthesis by two different polymerase complexes at the SV40 DNA replication origin.
Enzymatic synthesis of DNA from the simian virus 40 origin of DNA replication has been reconstituted in vitro with eight purified components. DNA polymerase alpha-primase complex first initiates DNA synthesis at the replication origin and continues as the lagging strand polymerase. Subsequently, the DNA polymerase delta complex initiates replication on the leading strand template. Some prokaryotic DNA polymerase complexes can replace the eukaryotic polymerase delta complex. A model for polymerase switching during initiation of DNA replication is presented.
Tsurimoto
T
T
Cold Spring Harbor Laboratory, New York 11724.
Melendy
T
T
Stillman
B
B
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000378:metabolism; D004258:DNA Polymerase III; D019915:DNA Primase; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D007700:Kinetics; D008957:Models, Genetic; D012316:RNA Nucleotidyltransferases / Q000378:metabolism; D013539:Simian virus 40 / Q000235:genetics*; D013950:Thymus Gland / Q000201:enzymology
D019915:DNA Primase; D012316:RNA Nucleotidyltransferases; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase