Molecular phenotype of a pediatric small round cell tumor.
Molecular probes were used to characterize an unusual small round cell abdominal tumor arising from the fallopian tube of a 15-year-old girl. DNA and RNA extracted from the tumor and adjacent normal tissue was subjected to Southern and Northern blot analysis using a variety of different probes. N-myc oncogene RNA was greatly expressed in the tumor, but was not expressed in normal tissue or amplified in chromosomal DNA. Insulin-like growth factor II RNA was similarly overexpressed in the neoplasm, but not in normal tissue. While histopathologic studies could not distinguish between a neuroectodermal neoplasm and Wilms' tumor, electron microscopy and the pattern of gene expression was most consistent with Wilms' tumor.
Bendit
I
I
Schneider Children's Hospital of Long Island Jewish Medical Center, New Hyde Park, New York.
Johnston
J
J
Valderrama
E
E
Waber
P
P
Nisen
P D
PD
D000293:Adolescent; D015152:Blotting, Northern; D015139:Blotting, Southern; D004273:DNA, Neoplasm / Q000032:analysis; D005185:Fallopian Tube Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D005784:Gene Amplification; D016259:Genes, myc; D006801:Humans; D010641:Phenotype; D012334:RNA, Neoplasm / Q000032:analysis; D012186:Retroperitoneal Neoplasms / Q000235:genetics* / Q000473:pathology; D009396:Wilms Tumor / Q000235:genetics* / Q000473:pathology
D004273:DNA, Neoplasm; D012334:RNA, Neoplasm