[Interactions of the phytopathogenic bacteria Erwinia with phage P1 CRL100 CML].
The bacteriophage P1 Cm clr100 lysogenises the bacteria E. chrysanthemi, E. aroideae, E. atroseptica being localized in the cytoplasm, replicating but causing no cell lysis. The prophage induction results in transformation of the lysogenic bacteria E. chrysanthemi into nonviable filamentous cells. However, a portion of cells gets rid of the prophage and gives rise to normal heritage inheritors permitting to use the bacteriophage as an efficient vehicle for introducing the transposons into the chromosome of E. chrysanthemi. The transposon Tn9 has been found to insert into the different chromosomal sites causing no inactivation of the genes, while the transposition of Tn5 from the bacteriophage P1::Tn5Cmclr100 induces the different mutations with the frequency up to 3%. The bacteriophage P1Cmclr100 may also serve a tool for construction of the homology regions in the chromosome of E. chrysanthemi and Flac+ plasmid for further construction of Hfr-type donors.
Beliasova
N A
NA
Prokulevich
V A
VA
Fomichev
Iu K
IuK
D001435:Bacteriophages / Q000235:genetics*; D004251:DNA Transposable Elements; D004885:Erwinia / Q000235:genetics* / Q000254:growth & development; D005822:Genetic Vectors; D008242:Lysogeny
D004251:DNA Transposable Elements
Osobennosti vzaimodeĭstviia fitopatogennykh bakteriĭ Errwinia s fagom P1 CRL100 CML.