Increased nerve growth factor receptor mRNA in contused rat spinal cord.
Peripheral nerve injury induces the expression of nerve growth factor receptor (NGFR). To determine whether a similar induction results from injury of the spinal cord, NGFR mRNA content was determined using Northern blot hybridization analysis of total RNA from spinal cords of rats contused in the mid-thoracic region. By four days after contusion NGFR mRNA was significantly increased in the thoracic segments that included the injury site. The induction was maximal at 7 days, about 5- to 7-fold the level of uninjured controls, and remained 4 times higher than controls at 14 and 28 days after injury. These results suggest that axotomy in the CNS may also trigger the molecular mechanism(s) leading to up-regulation of NGFR expression.
Brunello
N
N
Department of Anatomy and Cell Biology, Georgetown University, School of Medicine, Washington, DC 20007.
Reynolds
M
M
Wrathall
J R
JR
Mocchetti
I
I
D000818:Animals; D003288:Contusions / Q000378:metabolism*; D005260:Female; D009414:Nerve Growth Factors; D009416:Nerve Regeneration / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000235:genetics*; D017475:Receptors, Nerve Growth Factor; D013119:Spinal Cord Injuries / Q000378:metabolism*
D009414:Nerve Growth Factors; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor