A general method for the transfer of plasmid-borne mutant alleles to the chromosome of Klebsiella pneumoniae using bacteriophage lambda: transfer of pqq genes.
A generally applicable method is described for reintroduction of mutant plasmid-borne alleles to the chromosome of Klebsiella pneumoniae using bacteriophage lambda. We used this method to make stable chromosomal transposon insertions in genes for biosynthesis of pyrroloquinoline quinone in K. pneumoniae.
Meulenberg
J J
JJ
E.C. Slater Institute for Biochemical Research, University of Amsterdam, The Netherlands.
Loenen
W A
WA
Sellink
E
E
Postma
P W
PW
D000483:Alleles; D010582:Bacteriophage lambda / Q000235:genetics; D002876:Chromosomes, Bacterial / Q000378:metabolism*; D003067:Coenzymes / Q000378:metabolism; D004251:DNA Transposable Elements; D007711:Klebsiella pneumoniae / Q000235:genetics*; D009154:Mutation; D045542:PQQ Cofactor; D010957:Plasmids; D015363:Quinolones / Q000378:metabolism*; D015183:Restriction Mapping
D003067:Coenzymes; D004251:DNA Transposable Elements; D015363:Quinolones; D045542:PQQ Cofactor