Effect of cytokines on proliferation of Epstein Barr virus-transformed B lymphocytes.
Plating efficiencies of EBV transformed human B cells seeded in single cell cultures are far lower (less than 1%) than observed in T cell cloning experiments. This report describes the stimulatory effect of several crude as well as recombinant growth factors on proliferation of EBV transformed B cells measured by [3H]thymidine uptake. Supernatant of LPS activated monocytes (HSF) and recombinant interleukin 4 (rIL-4), but not recombinant IL-1 beta, IL-2, IL-6, TNF alpha, GM-CSF, and interferon gamma increased [3H]thymidine incorporation. The combination of HSF and rIL-4 was found to be synergistic on B cell proliferation. Plating efficiency of EBV transformed B cells at limiting dilution was improved by HSF, but not by the combination of HSF and rIL-4.
Richter
W
W
Department of Internal Medicine, University of Ulm, West Germany.
Eiermann
T H
TH
Scherbaum
W A
WA
D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D001685:Biological Factors / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D003470:Culture Media; D016207:Cytokines; D004854:Herpesvirus 4, Human; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D008213:Lymphocyte Activation; D009000:Monocytes / Q000276:immunology
D001685:Biological Factors; D003470:Culture Media; D016207:Cytokines; D015850:Interleukin-6; D015847:Interleukin-4