[Application of "mild" periodic acid oxidation to the ultrahistochemical detection of sialic acid-containing compounds in human fallopian tube epithelium].
2 histochemical methods were used to analyse the carbohydrates in the human oviductal epithelium, namely the periodic acid-semicarbazide-silver proteinate and the 'mild' periodic acid-thiosemicarbazide-silver proteinate technique. It was possible to detect periodate reactive substances and sialic acid-containing compounds, namely separately. The 'mild' periodic acid oxidation is a selective technique for the histochemical demonstration of the existence of unsubstituted acylneuraminic acids. The ultrahistochemical results show that, firstly, periodate reactive particles are located in the cytoplasm of ciliated and nonciliated cells. The material appears in form of beta-granula (glycogen). Secondly, we could observe fine granular reactive products in the lysosomal-like and lipid droplet-like structures. In these subjects the positive granula encircling ring-like the homogeneous matrix occur in the peripheral zone of the morphological substrate investigated. By the way, these granula situated in the periphery of organelles are alone reacting after staining with 'mild' periodic acid-thiosemicarbazide-silver proteinate; this means, the glycogen particles are unstained. Thus the findings indicate that, in the cells of the human oviductal epithelium, sialic acid-containing compounds are ultrahistochemically visualized by 'mild' periodic acid oxidation in combination with thiosemicarbazide and silver proteinate.
Schultka
R
R
Institut für Anatomie, Martin-Luther-Universität Halle-Wittenberg zu Halle.
Cech
S
S
D002923:Cilia / Q000648:ultrastructure; D004848:Epithelium / Q000648:ultrastructure; D005187:Fallopian Tubes / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D008854:Microscopy, Electron; D010504:Periodic Acid; D012794:Sialic Acids / Q000032:analysis*
D007202:Indicators and Reagents; D012794:Sialic Acids; D010504:Periodic Acid
Anwendung der "milden" Perjodsäure-Oxydation zum ultrahistochemischen Nachweis von sialinsäure-haltigen Verbindungen im menschlichen Eileiterepithel.