title: Apatite--wollastonite containing glass ceramic granule-fibrin mixture as a bone graft filler: use with low granular density.
abstract: The purpose of this study was to investigate bone formation after implantation of a mixture of apatite--wollastonite containing glass ceramic granules (A-W.GC, 220-355 microns) and fibrin glue with a low A-W.GC granular density. The fibrinogen was prepared from rat blood plasma. A mixture of A-W.GC granules and fibrin glue 1:4 in volume ratio (1:4 mixture) was implanted in the proximal metaphysis of the rat tibia, and bone formation was evaluated quantitatively by histomorphometry in undecalcified specimens. Microangiography was performed in animals sacrificed 5 days after the operation. The results were compared with those of a previous study in which a 1:1 mixture of A-W.GC granules and fibrin glue was used. With the 1:4 mixture, the A-W.GC density was 39.7% because of concentration of fibrin at packing. Good early vascularization comparable to that after implantation of the 1:1 mixture was observed. Bone formation proceeded at the same rate as after implantation of 1:1 mixture, but the level of the plateau was higher than that for the 1:1 mixture. The osteoconductive potential of the 1:4 A-W.GC-fibrin mixture was similar to that of the 1:1 mixture, and was higher than that of A-W.GC without fibrin.
authors.affiliation: Department of Orthopaedic Surgery, Faculty of Medicine, Kyoto University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ono
authors.forename: T
authors.initials: T
authors.lastname: Yamamuro
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Kokubo
mesh_terms: D000818:Animals; D001031:Apatites; D001672:Biocompatible Materials; D001846:Bone Development; D001842:Bone and Bones / Q000033:anatomy & histology / Q000098:blood supply / Q000502:physiology; D017610:Calcium Compounds; D002516:Ceramics; D005337:Fibrin; D005898:Glass; D008297:Male; D008856:Microscopy, Fluorescence; D019736:Prostheses and Implants; D051381:Rats; D011919:Rats, Inbred Strains; D017640:Silicates; D012824:Silicic Acid; D012822:Silicon Dioxide
chemical_list: D001031:Apatites; D001672:Biocompatible Materials; D017610:Calcium Compounds; D017640:Silicates; D012824:Silicic Acid; D012822:Silicon Dioxide; D005337:Fibrin; C031293:calcium silicate