title: Membrane-associated phosphoinositidase C activity in Dictyostelium discoideum.
abstract: Membrane-associated phosphoinositidase C activity has been identified in Dictyostelium discoideum using phosphatidylinositol 4,5-bisphosphate as exogenous substrate. Maximal activity was observed with 0.4 mM phosphatidylinositol 4,5-bisphosphate at pH 7.0. The enzyme was stimulated by micromolar concentrations of free calcium with maximal activity at 100 microM.
authors.affiliation: Biochemistry Department, University of Oxford, UK.
authors.forename: G A
authors.initials: GA
authors.lastname: Lundberg
authors.forename: P C
authors.initials: PC
authors.lastname: Newell
mesh_terms: D002118:Calcium / Q000502:physiology; D002462:Cell Membrane / Q000201:enzymology; D002852:Chromatography, Ion Exchange; D004023:Dictyostelium / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology
chemical_list: D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols; D010727:Phosphoric Diester Hydrolases; C021491:glycerophosphoinositol glycerophosphodiesterase; D002118:Calcium