Integrated HPV 1 genomes in a human keratinocyte cell line can be transactivated by a SV40/BPV1 recombinant virus which expresses BPV1 E2 proteins.
This paper describes studies carried out on an HPV 1 carrying human keratinocyte cell line (SVD2) and two subclones of it. Although these lines contain multiple copies of HPV 1 genomes, in situ hybridization revealed that integration was restricted to band q33 on the long arms of chromosome 2. An E4 1.25-kb mRNA was specifically identified by Northern blotting and a PCR generated cDNA confirmed the presence of the E1/E4 spliced mRNA which is abundant in HPV 1 containing papillomas. Infection of an SVD2 subclone (SVD2 (cyst) sp) with a SV40/BPV1 recombinant virus which expresses the BPV1 E2 48-kb transactivator protein enhanced HPV 1 transcription at least 20-fold.
Parton
A
A
Department of Cancer Studies, University of Birmingham Medical School, England, United Kingdom.
Grand
R J
RJ
Biggs
P
P
Settleman
J
J
DiMaio
D
D
Gallimore
P H
PH
D002461:Cell Line, Transformed; D002999:Clone Cells; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D006801:Humans; D007621:Karyotyping; D015603:Keratinocytes / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013539:Simian virus 40 / Q000235:genetics; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014162:Transfection; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics
D004274:DNA, Recombinant; D004279:DNA, Viral; D004268:DNA-Binding Proteins; C051550:E2 protein, Bovine papillomavirus; D011994:Recombinant Proteins; D014764:Viral Proteins