Comparisons of the genomic RNA of Arkansas DPI embryonic passages 10 and 100, Australian T, and Massachusetts 41 strains of infectious bronchitis virus.
The genomic ribonucleic acid (RNA) of Arkansas DPI at embryonic passages 10 and 100, Australian T, and Massachusetts 41 strains of infectious bronchitis virus (IBV) were compared using two-dimensional, 32PpCp-labeled oligonucleotide fingerprinting. The Arkansas DPI strain embryonic passage 10 was pathogenic for chickens and had one oligonucleotide not present in the attenuated passage 100. The remaining Arkansas DPI oligonucleotides had identical electrophoretic mobility. The fingerprint patterns of the Australian T, Massachusetts 41, and Arkansas DPI IBV genomes were dissimilar, thus indicating they were genetically distinct. The distinctive fingerprint patterns of these virus strains, all identified as IBV by other criteria, indicate considerable genomic variation. Fingerprinting is reliable and sensitive, and should be used in combination with other characterization methods for identifying and differentiating IBV strains.
Butcher
G D
GD
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville 32610.
Gelb
J
J
Collisson
E W
EW
D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D003332:Coronaviridae / Q000235:genetics*; D003333:Coronaviridae Infections / Q000382:microbiology / Q000662:veterinary; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D001351:Infectious bronchitis virus / Q000235:genetics*; D009710:Nucleotide Mapping; D011201:Poultry Diseases / Q000382:microbiology; D012367:RNA, Viral / Q000032:analysis*; D013047:Specific Pathogen-Free Organisms
D012367:RNA, Viral