title: Identification of individual renocortical cells that secrete renin.
abstract: Successful application of the reverse hemolytic plaque assay was developed to identify individual renocortical cells that secrete renin directly. The plaque assay was validated by a number of established criteria. Using this technique, we demonstrate an increase in renin secretion with beta-adrenergic stimulation and an inhibition of renin secretion with extracellular calcium in groups of renin-secreting cells. Transmission electron microscopy of the cell in the center of a hemolytic plaque demonstrated a modified vascular smooth muscle cell with densely packed secretory granules. Electron microscopy immunocytochemistry demonstrated the presence of renin in the secretory granules, confirming the identity of the cell as a renal juxtaglomerular cell. The technology developed here has allowed the precise identification and study of the individual renin-secreting juxtaglomerular cell.
authors.affiliation: Department of Internal Medicine, University of Virginia School of Medicine, Charlottesville 22908.
authors.forename: R M
authors.initials: RM
authors.lastname: Carey
authors.forename: K M
authors.initials: KM
authors.lastname: Geary
authors.forename: M K
authors.initials: MK
authors.lastname: Hunt
authors.forename: S P
authors.initials: SP
authors.lastname: Ramos
authors.forename: M S
authors.initials: MS
authors.lastname: Forbes
authors.forename: T
authors.initials: T
authors.lastname: Inagami
authors.forename: M J
authors.initials: MJ
authors.lastname: Peach
authors.forename: D A
authors.initials: DA
authors.lastname: Leong
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D006046:Gold; D006462:Hemolytic Plaque Technique; D007150:Immunohistochemistry; D007545:Isoproterenol / Q000494:pharmacology; D007672:Kidney Cortex / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009994:Osmolar Concentration; D051381:Rats; D011921:Rats, Inbred WKY; D012083:Renin / Q000378:metabolism*; D013205:Staphylococcal Protein A
chemical_list: D013205:Staphylococcal Protein A; D006046:Gold; D012083:Renin; D007545:Isoproterenol; D002118:Calcium