Production of metastatic phenotype with DNA from metastatic cells but not with oncogenic DNA.
Although the tumorigenic phenotype can be transferred by transfecting DNA from a variety of human tumours into 3T3 mouse fibroblasts, the ability to transfer the metastatic phenotype in a similar manner in syngeneic animals is largely unknown. We now report that transfection of a rat mammary epithelial cell line Rama 37, which produces nonmetastasizing adenomatous tumours in syngeneic rats, with DNA from a metastasizing rat mammary cell line, Rama 800, yields some transfectants with reproducible metastatic capabilities. Nonspecific DNA or the oncogenes EJ-ras-1 or Polyoma Large T Antigen fail in this respect, although their transfectants often possess increased tumorigenic potential.
Jamieson
S
S
Biochemistry Department, Liverpool University, U.K.
Barraclough
R
R
Rudland
P S
PS
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000502:physiology*; D004279:DNA, Viral / Q000502:physiology; D005260:Female; D011905:Genes, ras / Q000235:genetics*; D009362:Neoplasm Metastasis / Q000235:genetics*; D009368:Neoplasm Transplantation; D010641:Phenotype; D051381:Rats; D011920:Rats, Inbred WF; D014162:Transfection; D014407:Tumor Cells, Cultured
D000952:Antigens, Polyomavirus Transforming; D004273:DNA, Neoplasm; D004279:DNA, Viral; D004262:DNA Restriction Enzymes