Beta 1 subunits of sodium channels. Studies with subunit-specific antibodies.
The purified Na+ channel from rat brain consists of alpha (260 kDa), beta 1 (36 kDa), and beta 2 (33 kDa) subunits. Pure beta 1 subunits were prepared from purified rat brain Na+ channels which had been adsorbed to hydroxylapatite resin and used to prepare specific anti-beta 1 subunit antiserum. Antibodies purified from this antiserum by antigen affinity chromatography immunoprecipitate 125I-labeled, purified beta 1 subunits and purified Na+ channels (measured as high affinity [3H] saxitoxin binding sites) and recognize beta 1 subunits on immunoblots of solubilized rat brain membranes. The affinity-purified anti-beta 1 antibodies recognize beta 1 subunits in rat spinal cord, heart, skeletal muscle, and sciatic nerve, but do not detect immunoreactive beta 1 subunits in eel electroplax or eel brain. The developmental time course of expression of immunoreactive beta 1 subunits in rat forebrain was measured by immunoprecipitation followed by immunoblotting with affinity-purified anti-beta 1 antibodies. The amount of immunoreactive beta 1 subunits increased steadily to adult levels during the first 21 days of postnatal development.
Sutkowski
E M
EM
Department of Pharmacology, University of Washington School of Medicine, Seattle 98195.
Catterall
W A
WA
D000367:Age Factors; D029845:Amphibian Proteins; D000818:Animals; D001921:Brain / Q000254:growth & development; D002352:Carrier Proteins / Q000276:immunology; D046911:Macromolecular Substances; D008970:Molecular Weight; D011233:Precipitin Tests; D051381:Rats; D015222:Sodium Channels / Q000276:immunology* / Q000378:metabolism / Q000648:ultrastructure; D014018:Tissue Distribution
D029845:Amphibian Proteins; D002352:Carrier Proteins; D046911:Macromolecular Substances; D015222:Sodium Channels; C027525:saxitoxin-binding protein, Rana catesbeiana