title: Effect of gangliosides on membrane permeability studied by enzymic and fluorescence-spectroscopy techniques.
abstract: The effect of gangliosides on membrane permeability was investigated by studying the kinetic properties of cytochrome c oxidase, the activity of which, when the enzyme is reconstituted in phospholipid vesicles, is dependent on membrane permeability to H+ and K+. The experiments indicate that three different gangliosides (GM1, DD1a, GT1b) incorporated into cytochrome c oxidase-containing phospholipid vesicles stimulate enzymic activity, in the absence of ionophores, most probably by disorganizing the bilayer lipid assembly and increasing its permeability to ions. This interpretation was confirmed by fluorescence-spectroscopy experiments in which the rate of passive leakage of carboxyfluorescein entrapped in the vesicles was measured. Cholera toxin, or its isolated B-subunit, added to GM1-containing proteoliposomes inhibited cytochrome c oxidase activity, indicating the lack of formation, under these experimental conditions, of channels freely permeable to H+ or K+.
authors.affiliation: Center of Molecular Biology (CNR), University of Rome Tor Vergata, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Sarti
authors.forename: G
authors.initials: G
authors.lastname: Antonini
authors.forename: F
authors.initials: F
authors.lastname: Malatesta
authors.forename: B
authors.initials: B
authors.lastname: Vallone
authors.forename: M
authors.initials: M
authors.lastname: Brunori
authors.forename: M
authors.initials: M
authors.lastname: Masserini
authors.forename: P
authors.initials: P
authors.lastname: Palestini
authors.forename: G
authors.initials: G
authors.lastname: Tettamanti
mesh_terms: D003576:Electron Transport Complex IV / Q000378:metabolism*; D005732:Gangliosides; D007700:Kinetics; D008081:Liposomes; D008954:Models, Biological; D010539:Permeability; D010713:Phosphatidylcholines; D010743:Phospholipids; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship
chemical_list: D005732:Gangliosides; D008081:Liposomes; D010713:Phosphatidylcholines; D010743:Phospholipids; C024990:asolectin; D003576:Electron Transport Complex IV