[The trigger function of cAMP in regulating the chemiluminescence of macrophages exposed to ethanol].
The capacity of mouse peritoneal macrophages to luminol-dependent zymosan-induced chemiluminescence was investigated. It was revealed that a short-term incubation (5-10 min) of macrophages in 0.05-0.50% ethanol resulted in a dose-dependent reduction of chemiluminescent response. Ethanol induce a sharp 2-3 fold increase in the intracellular cAMP content after a 2 minutes incubation. The temporal and concentration correlation of both the processes suggests that the inhibition of chemiluminescence may be mediated by the intracellular cAMP content.
Tokmanov
A A
AA
Denisenko
V Iu
VIu
Vasil'ev
V Iu
VIu
D000818:Animals; D002478:Cells, Cultured / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D000242:Cyclic AMP / Q000032:analysis / Q000502:physiology*; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology*; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology; D008264:Macrophages / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D013997:Time Factors; D015054:Zymosan / Q000494:pharmacology
D000431:Ethanol; D008165:Luminol; D015054:Zymosan; D000242:Cyclic AMP
Triggernaia funktsiia tsAMF v reguliatsii khemiliuminestsentsii makrofagov pri vozdeĭstvii étanola.