Ultraviolet light-induced DNA damage in transcribed sequences: no change in repair with age.
We studied the repair of a plasmid vector containing the chloramphenicol acetyltransferase (CAT) gene by treating the plasmid with UV light and then transfecting this plasmid into fibroblasts from human fetal lung (in vitro aging) and into primary cultured fibroblasts from rat lung and skin. This methodology allows us to examine the repair of specific transcribed DNA sequences. There was no age-related change in the repair of UV damage in these cells. Rat embryo fibroblasts at different passages transfected with the plasmid also revealed no significant alteration in UV repair as a function of passage number.
Kunisada
T
T
Laboratory of Molecular Genetics, National Institute on Aging, Baltimore, MD 21224.
Miller
C D
CD
Schneider
E L
EL
D000375:Aging / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004249:DNA Damage; D004260:DNA Repair / Q000502:physiology*; D005822:Genetic Vectors; D006801:Humans; D011401:Promoter Regions, Genetic; D051381:Rats; D011919:Rats, Inbred Strains; D013539:Simian virus 40 / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics*; D014162:Transfection; D014466:Ultraviolet Rays
D015500:Chloramphenicol O-Acetyltransferase