title: Murine leukemia inhibitory factor enhances retroviral-vector infection efficiency of hematopoietic progenitors.
abstract: We have investigated the in vitro effects of the cytokine leukemia inhibitory factor (LIF) on normal murine hematopoietic progenitors by measuring recovery and retroviral vector infection efficiency of 13-day posttransplant, spleen-colony-forming cell (CFU-S 13) in short-term culture. Up to a twofold increase in CFU-S13 recovery was observed, from 9.7 x 10(-5) cells in untreated controls to 17.8 to 19.5 x 10(-5) cells, depending on the concentration of LIF. Histologic analysis of spleen colonies from control and LIF-treated marrows demonstrated that there was no detectable alteration in the differentiative potential of CFU-S13. The efficiency of CFU-S13 infection was increased from 15% in untreated controls to 84% to 91% in LIF-treated marrows. Analysis of proviral integration sites in spleen colonies indicated that some CFU-S13 precursors were infected in the LIF-treated marrows.
authors.affiliation: Howard Hughes Medical Institute, Houston, TX.
authors.forename: F A
authors.initials: FA
authors.lastname: Fletcher
authors.forename: D E
authors.initials: DE
authors.lastname: Williams
authors.forename: C
authors.initials: C
authors.lastname: Maliszewski
authors.forename: D
authors.initials: D
authors.lastname: Anderson
authors.forename: M
authors.initials: M
authors.lastname: Rives
authors.forename: J W
authors.initials: JW
authors.lastname: Belmont
mesh_terms: D000818:Animals; D001483:Base Sequence; D001853:Bone Marrow / Q000382:microbiology / Q000502:physiology; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000382:microbiology / Q000502:physiology; D005822:Genetic Vectors / Q000502:physiology*; D006131:Growth Inhibitors / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000382:microbiology* / Q000502:physiology; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines; D051379:Mice; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000235:genetics; D016376:Oligonucleotides, Antisense; D012192:Retroviridae Infections / Q000235:genetics / Q000503:physiopathology*; D013154:Spleen / Q000166:cytology / Q000382:microbiology / Q000502:physiology; D013234:Stem Cells / Q000166:cytology / Q000382:microbiology / Q000502:physiology
chemical_list: D006131:Growth Inhibitors; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense