title: A human homologue of the yeast HDEL receptor.
abstract: Retention of resident proteins in the lumen of the endoplasmic reticulum is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually Lys-Asp-Glu-Leu (KDEL in the single letter code) in animal cells, His-Asp-Glu-Leu (HDEL) in Saccharomyces cerevisiae. There is evidence that the ERD2 gene encodes the sorting receptor that recognizes HDEL in yeast; its product is an integral membrane protein of relative molecular mass 26,000 (26K) that is not glycosylated. In contrast, Vaux et al. suggest that the mammalian KDEL receptor is a 72K glycoprotein that they detected using an anti-idiotypic antibody approach. If this were so, it would indicate a surprising divergence of the retrieval machinery between yeast and animal cells. We report here that human cells express a protein similar in sequence, size and properties to the ERD2 product, and propose that this protein is the human KDEL receptor.
authors.affiliation: MRC Laboratory of Molecular Biology, Cambridge, UK.
authors.forename: M J
authors.initials: MJ
authors.lastname: Lewis
authors.forename: H R
authors.initials: HR
authors.lastname: Pelham
mesh_terms: D000595:Amino Acid Sequence; D002451:Cell Compartmentation; D003001:Cloning, Molecular; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D007716:Kluyveromyces / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011956:Receptors, Cell Surface / Q000502:physiology*; D018000:Receptors, Peptide; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D012995:Solubility; D014162:Transfection
chemical_list: C064922:ERD2 protein, S cerevisiae; C489304:KDELR1 protein, human; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D018000:Receptors, Peptide; D029701:Saccharomyces cerevisiae Proteins