title: Gastrulation in the sea urchin Strongylocentrotus purpuratus is blocked by the fluorescein dye erythrosin B.
abstract: Erythrosin B, a food, drug, and cosmetic dye, arrested gastrulation in embryos of the sea urchin Strongylocentrotus purpuratus. A 30 min pulse of 5 microM erythrosin B added at 18 hr postinsemination blocked gastrulation scored at 50 hr postinsemination when control embryos had completed gastrulation. Dye addition at later times had no detectable effects on development through 50 hr postinsemination. The dye may block primary invagination via its known effects on plasma membrane permeability and fluidity.
authors.affiliation: Department of Biology, University of California, Riverside 92521.
authors.forename: E J
authors.initials: EJ
authors.lastname: Carroll
mesh_terms: D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D004396:Coloring Agents / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004923:Erythrosine / Q000008:administration & dosage / Q000494:pharmacology*; D005452:Fluoresceins / Q000008:administration & dosage / Q000494:pharmacology*; D005775:Gastrula / Q000187:drug effects*; D012617:Sea Urchins / Q000502:physiology*
chemical_list: D004396:Coloring Agents; D005452:Fluoresceins; D004923:Erythrosine