title: Effects of ozone treatment on the infectivity of hepatitis A virus.
abstract: The inactivation of a large-focus-forming variant of hepatitis A virus (HM-175) by ozone was investigated. Experiments using mainly single-particle virus preparations suspended in phosphate-carbonate buffer were conducted over a range of pH levels (6-8) at 4 degrees C. Viral enumerations involved the use of a radioimmunofocus assay. While some tolerance to lower (i.e., 0.1-0.5 mg/L) ozone residuals was noted, the exposure of virus particles to ozone concentrations of 1 mg/L or greater at all pH levels resulted in their complete (5 log) inactivation within 60 s. The pH-related effects that were observed were not considered to be significant.
authors.affiliation: Department of Microbiology, University of New England College of Medicine, Biddeford, ME 04005.
authors.forename: J M
authors.initials: JM
authors.lastname: Vaughn
authors.forename: Y S
authors.initials: YS
authors.lastname: Chen
authors.forename: J F
authors.initials: JF
authors.lastname: Novotny
authors.forename: D
authors.initials: D
authors.lastname: Strout
mesh_terms: D000818:Animals; D002021:Buffers; D002460:Cell Line; D002522:Chlorocebus aethiops; D006507:Hepatovirus / Q000187:drug effects* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D007668:Kidney; D010126:Ozone / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D002021:Buffers; D010126:Ozone