Autoradiographic localization of specific atrial natriuretic peptide binding sites on immunocytochemically identified cells in cultures from rat and guinea-pig hearts.
Dissociated cell culture preparations from rat and guinea-pig atria and interatrial septum, and from rat ventricles were studied using a combined autoradiographic and immunocytochemical approach. Alpha-atrial natriuretic peptide (125I-ANP1-128) bindings sites were confined to subpopulations of identified non-neuronal cells in each type of culture preparation, and had distinct patterns of labelling. The density of ANP1-28 binding sites was substantially greater in guinea-pig cultures than in rat cultures and was least in rat ventricular cultures. ANP1-28-labelled subpopulations of S-100-like immunoreactive glial cells were only seen in guinea-pig cultures. Von Willebrand factor (vWF)-like immunoreactive endothelial cells and vWF-negative endothelioid cells expressed ANP1-28 binding sites in both the guinea-pig and rat atrial cultures, but were unlabelled in rat ventricular cultures. In contrast, labelled subpopulations of fibronectin-like immunoreactive fibroblasts were present in all of the three types of culture preparation studied. ANP-like immunoreactive myocytes were present in both atrial and ventricular cultures. These cells did not, however, express ANP1-28 binding sites.
James
S
S
Department of Anatomy and Developmental Biology, University College London, United Kingdom.
Hassall
C J
CJ
Polak
J M
JM
Burnstock
G
G
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D001345:Autoradiography; D002478:Cells, Cultured; D004699:Endocardium / Q000648:ultrastructure; D005347:Fibroblasts / Q000032:analysis; D006168:Guinea Pigs; D006325:Heart Atria; D006346:Heart Septum / Q000166:cytology; D006352:Heart Ventricles; D009206:Myocardium / Q000166:cytology*; D051381:Rats; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000032:analysis*; D013045:Species Specificity; D014841:von Willebrand Factor / Q000032:analysis
D011956:Receptors, Cell Surface; D014841:von Willebrand Factor; D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor