Physical mapping and molecular cloning of mung bean yellow mosaic virus DNA.
Viral single-stranded DNA of mung bean yellow mosaic virus (MYMV) was converted to the double-stranded state in vitro, and physical mapping was carried out. The genome of MYMV was found to consist of two major components (designated as DNA 1 and DNA 2). In addition, some minor components were detected. Molecular cloning of the major components was carried out, using in vitro double-stranded DNA and replicative intermediate DNAs. DNA 1 is about 2.72 and DNA 2 about 2.67 kilobase pairs. No similarities were observed when the two restriction maps of DNA 1 and 2 were compared.
Morinaga
T
T
Biotechnology Research Laboratory, Teijin Tokyo Research Center, Japan.
Ikegami
M
M
Miura
K
K
D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D007887:Fabaceae / Q000382:microbiology*; D005814:Genes, Viral; D009029:Mosaic Viruses / Q000235:genetics*; D010946:Plants, Medicinal; D015183:Restriction Mapping
D004279:DNA, Viral; D004262:DNA Restriction Enzymes