ESR spin trapping and NMR spectroscopy of the same heart shows correlation between energy depression and radical formation during postischemic reperfusion.
The relevance of radical formation in disturbances of energy metabolism in the postischemic heart is not clear. This study provides the first evidence of a significant correlation between the amount of oxy-radicals trapped in the effluent of isolated hearts upon reperfusion and the decreased myocardial content of phosphocreatine and ATP. This suggests that the loss of high-energy compounds might contribute to oxy-radical production during reperfusion. The application of ESR spin trapping and of NMR technique to the same heart is a new approach to investigate the pathobiochemical relevance of free radicals for the heart muscle.
Blasig
I E
IE
Institute of Drug Research, Academy of Sciences of the GDR, Berlin.
Steinschneider
A Y
AY
Lakomkin
V L
VL
Ledenev
A N
AN
Korchazhkina
O V
OV
Ruuge
E K
EK
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D003327:Coronary Disease / Q000175:diagnosis / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D005609:Free Radicals; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D015425:Myocardial Reperfusion; D010725:Phosphocreatine / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D005609:Free Radicals; D010725:Phosphocreatine; D000255:Adenosine Triphosphate