[The comparative characteristics of the localization of the activity of alkaline phosphatase and ouabain-sensitive, potassium-dependent p-nitrophenyl phosphatase in the myocardium of white rats at the ultrastructural level].
A comparative characteristic of alkaline phosphatase and Na(+)-K(+)-ATPase localization activity within white rat myocardium is presented at the ultrastructural level. Both different in principle and common features of the enzyme reactional products precipitation are revealed. The original technique is used to determine ouabain-sensitive potassium-dependent p-nitrophenylphosphatase part of Na(+)-K(+)-ATPase complex at physiological pH. The verification of the main characteristics of Na(+)-K(+)-ATPase complex membrane localization activity within the rat myocardium using this cytochemical procedure are discussed.
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D009597:4-Nitrophenylphosphatase / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D002021:Buffers; D006651:Histocytochemistry; D006863:Hydrogen-Ion Concentration; D007854:Lead / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000201:enzymology* / Q000648:ultrastructure; D009566:Nitrates / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013379:Substrate Specificity / Q000187:drug effects
D002021:Buffers; D009566:Nitrates; D007854:Lead; D010042:Ouabain; C017461:lead nitrate; D000469:Alkaline Phosphatase; D010744:Phosphoric Monoester Hydrolases; D009597:4-Nitrophenylphosphatase; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium
Sravnitel'naia kharakteristika lokalizatsii aktivnosti shchelochnoĭ fosfatazy i ouabain-chuvstvitel'noĭ, kaliĭ-zavisimoĭ p-nitrofenilfosfatazy v miokarde belykh krys na ul'trastrukturnom urovne.