title: A K2 neutral Saccharomyces cerevisiae strain contains a variant K2 M genome.
abstract: K2 neutral strain Saccharomyces cerevisiae USM12 was identified and characterized. This strain carried an M double-stranded RNA (dsRNA) genome encoding for resistance to K2 toxin. The M dsRNA was larger than the K2 killer yeast M dsRNA and homoduplex analysis of denatured and reannealed K2 neurtal M dsRNA revealed an inverted duplication. Heteroduplex analysis showed that two thirds of the K2 M genome had homology with the K2 neutral M genome. Hybridization showed that the USM12 M dsRNA had significant homology with the K2 M dsRNA. Protein profiles of extracellular proteins from USM12 and a cured strain indicated that USM12 did not secrete any toxin. This is the first time that a K2 neutral yeast strain has been characterized.
authors.affiliation: Department of Microbiology, University of Stellenbosch, South Africa.
authors.forename: B D
authors.initials: BD
authors.lastname: Wingfield
authors.forename: V J
authors.initials: VJ
authors.lastname: Southgate
authors.forename: I S
authors.initials: IS
authors.lastname: Pretorius
authors.forename: H J
authors.initials: HJ
authors.lastname: van Vuuren
mesh_terms: D003513:Cycloheximide / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D005800:Genes, Fungal; D055646:Killer Factors, Yeast; D008854:Microscopy, Electron; D009183:Mycotoxins / Q000235:genetics / Q000494:pharmacology*; D009692:Nucleic Acid Heteroduplexes; D009693:Nucleic Acid Hybridization; D012330:RNA, Double-Stranded / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins
chemical_list: C527157:KHR1 protein, S cerevisiae; D055646:Killer Factors, Yeast; D009183:Mycotoxins; D009692:Nucleic Acid Heteroduplexes; D012330:RNA, Double-Stranded; D029701:Saccharomyces cerevisiae Proteins; D003513:Cycloheximide