title: A tripartite HIV-1 tat-env-rev fusion protein.
abstract: A 26 kd protein reactive with antiserum to the transactivator tat of the Human Immunodeficiency Virus Type 1 (HIV-1) has been detected in virus producing cells. The 26 kd protein is shown to be a tripartite fusion protein including coding sequences of the tat, envelope (env) and regulator of virion expression (rev) genes. Fusion of these coding sequences occurs by use of a previously undescribed exon within env. This 26 kd protein, designated tnv, has tat but no rev activity detectable with the assay used. The existence of other less abundant tat and rev related proteins in HIV-1 producing cells is also noted.
authors.affiliation: Dana-Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, MA 02115.
authors.forename: J
authors.initials: J
authors.lastname: Salfeld
authors.forename: H G
authors.initials: HG
authors.lastname: Göttlinger
authors.forename: R A
authors.initials: RA
authors.lastname: Sia
authors.forename: R E
authors.initials: RE
authors.lastname: Park
authors.forename: J G
authors.initials: JG
authors.lastname: Sodroski
authors.forename: W A
authors.initials: WA
authors.lastname: Haseltine
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D015723:Gene Library; D015694:Gene Products, rev / Q000032:analysis / Q000235:genetics*; D015696:Gene Products, tat / Q000032:analysis / Q000235:genetics*; D005814:Genes, Viral; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid; D015534:Trans-Activators / Q000235:genetics*; D014162:Transfection; D014759:Viral Envelope Proteins / Q000032:analysis / Q000235:genetics*; D054321:rev Gene Products, Human Immunodeficiency Virus; D054322:tat Gene Products, Human Immunodeficiency Virus
chemical_list: D015694:Gene Products, rev; D015696:Gene Products, tat; D015347:RNA Probes; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D014759:Viral Envelope Proteins; D054321:rev Gene Products, Human Immunodeficiency Virus; D054322:tat Gene Products, Human Immunodeficiency Virus