Thin-layer electrophoretic separation of monosaccharides, oligosaccharides and related compounds on reverse phase silica gel.
The electrophoretic mobilities of monosaccharides, oligosaccharides, sugar alcohols and sugar acids were determined in 0.3 M borate buffer, pH 10, using thin-layer electrophoresis on silanized silica gel, pretreated with octanol-1. A rapid separation of a number of sugars, occurring in foods, could be achieved. Using a 0.05-0.1 M neutral solution of barium acetate as electrolyte, thin-layer electrophoresis allowed excellent and rapid separation as well as identification of all common uronic acids which are constituents of many acidic polysaccharides.
Scherz
H
H
Deutsche Forschungsanstalt für Lebensmittelchemie, Garching, Federal Republic of Germany.
D004586:Electrophoresis; D005782:Gels; D009005:Monosaccharides / Q000302:isolation & purification*; D009844:Oligosaccharides / Q000302:isolation & purification*; D058428:Silica Gel; D012822:Silicon Dioxide; D013400:Sugar Acids / Q000302:isolation & purification; D013402:Sugar Alcohols / Q000302:isolation & purification; D013403:Sugar Phosphates / Q000302:isolation & purification
D005782:Gels; D009005:Monosaccharides; D009844:Oligosaccharides; D013400:Sugar Acids; D013402:Sugar Alcohols; D013403:Sugar Phosphates; D058428:Silica Gel; D012822:Silicon Dioxide