Nucleotide sequence of cDNA for nuclear encoded subunit Vb of mouse cytochrome-c oxidase.
We have used a polyclonal antibody probe to isolate cDNA clones for mouse cytochrome oxidase subunit Vb from a bone marrow tumor cell mRNA library in lambda gt11 expression vector. The mouse cDNA contains an open reading frame of 128 amino acids, which shows 81% positional identity with the predicted amino acid sequences of the human subunit. Northern blot analysis and sequencing of cDNA from a mouse kidney library show no tissue specific variations in subunit Vb.
Basu
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Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104.
Avadhani
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000201:enzymology; D004247:DNA / Q000032:analysis; D003576:Electron Transport Complex IV / Q000235:genetics*; D014644:Genetic Variation; D015698:Genomic Library; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D012333:RNA, Messenger / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D004247:DNA; D003576:Electron Transport Complex IV