One of the endothelin gene family, endothelin 3 gene, is expressed in the placenta.
A cDNA encoding human endothelin 3 (ET-3) precursor was cloned from a cDNA library from the placenta, and its nucleotide and deduced amino acid sequences were determined. This ET-3 cDNA was found to contain 2.3 kb pairs and encode prepro-ET-3 protein consisting of 224 amino acid residues. The putative big-ET-3 seems to consist of 42 amino acid residues. Two of the intron insertion sites were determined with information from nucleotide sequences of the cloned genomic ET-3 gene. This is the first direct evidence that the ET-3 gene is transcribed and expressed in the placenta.
Onda
H
H
Tsukuba Research Laboratories, Takeda Chemical Industries Ltd., Ibaraki, Japan.
Ohkubo
S
S
Ogi
K
K
Kosaka
T
T
Kimura
C
C
Matsumoto
H
H
Suzuki
N
N
Fujino
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D019332:Endothelin-1; D004730:Endothelium, Vascular; D005260:Female; D015870:Gene Expression; D006801:Humans; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010455:Peptides / Q000235:genetics*; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011498:Protein Precursors / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D014162:Transfection
D019332:Endothelin-1; D010455:Peptides; D011498:Protein Precursors; D004247:DNA