Serotyping of human group A rotavirus with oligonucleotide probes.
Rotaviruses (RV) in stools of children with diarrhea in Thailand were serotyped by monoclonal enzyme immunoassay (MEIA), and RNA extracted from these specimens were tested for hybridization with oligonucleotides constructed from the nucleotide sequences of VP7 of human serotypes 1, 2, 3, and 4. Of 178 specimens that contained RV as identified with a monoclonal antibody to group A RV, 84% (149/178) hybridized with serotype-specific oligonucleotides, and 42% (74/178) were serotyped by MEIA (P less than .001). Of the 74 specimens that were serotyped by MEIA, 92% (35/38) of type 1, 97% (34/35) of type 2, and the one type 4 RV hybridized with the HuG1Ac, HuG2Ac, and HuG4Ac oligonucleotides, respectively. RV strains identified in children in Thailand in 1987 and 1988 to which a serotype could be assigned by either method were either type 1, type 2, or, less often, type 4. Testing RV for hybridization with oligonucleotides for genes encoding VP7 is an alternate method of determining RV serotypes.
Sethabutr
O
O
Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
Unicomb
L E
LE
Holmes
I H
IH
Taylor
D N
DN
Bishop
R F
RF
Echeverria
P
P
D000911:Antibodies, Monoclonal; D001483:Base Sequence; D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D012367:RNA, Viral / Q000032:analysis*; D012401:Rotavirus / Q000145:classification* / Q000235:genetics; D012400:Rotavirus Infections / Q000453:epidemiology / Q000382:microbiology*; D012703:Serotyping; D013785:Thailand / Q000453:epidemiology
D000911:Antibodies, Monoclonal; D015345:Oligonucleotide Probes; D012367:RNA, Viral