In vitro synthesis of prostaglandin E and F2 alpha by pig endometrium in the presence of estradiol, catechol estrogen and ascorbic acid.
These experiments were undertaken to determine the potential for estradiol-17 beta (E2), 2-hydroxyestradiol-17 beta (2-OH-E2) and 4-hydroxyestradiol-17 beta (4-OH-E2) to regulate prostaglandin (PG) E and F2 alpha synthesis by pig endometrium. Endometrium was collected from pigs on d 10 of pregnancy and incubated (15 to 20 mg/well) for three 2-h periods in 2 ml of medium in 24-well culture plates. At the end of each period, the medium was removed and frozen. Later media were thawed and assayed for PGE and PGF2 alpha. During Periods 2 and 3, the medium contained 0, 25, 50, 100 or 150 microM 2-OH-E2 (Exp. 1); 0, 25 or 50 microM 4-OH-E2 (Exp. 2); or 0, 25 or 50 microM E2 (Exp. 3). Each experiment was a factorial with 2-OH-E2, 4-OH-E2 or E2 as one main effect and 0 or 1 mM ascorbate as a second main effect. Ascorbate decreased (P less than .01) PGE and PGF2 alpha release in all experiments. Two-hydroxyestradiol-17 beta decreased (P less than .01) PGE and PGF2 alpha release into the medium during Periods 2 and 3 in a dose-dependent manner (Exp. 1). In Exp. 2, 4-OH-E2 decreased (P less than .07) endometrial release of PGE and PGF2 alpha in Periods 2 and 3 and increased (P less than .01) the PGE:PGF2 alpha in Period 3. In Exp. 3, E2 decreased release of PGE during Period 3 and PGF2 alpha release during Period 2. The PGE:PGF2 alpha was not altered by E2.(ABSTRACT TRUNCATED AT 250 WORDS)
Rosenkrans
C F
CF
Dept. of Anim. Sci. and Ind., Kansas State University, Manhattan 66506.
Paria
B C
BC
Davis
D L
DL
Milliken
G
G
D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D015237:Dinoprost / Q000096:biosynthesis*; D004717:Endometrium / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism*; D002393:Estrogens, Catechol / Q000378:metabolism; D005260:Female; D011247:Pregnancy; D011458:Prostaglandins E / Q000096:biosynthesis*; D013552:Swine / Q000378:metabolism*
D002393:Estrogens, Catechol; D011458:Prostaglandins E; D004958:Estradiol; D015237:Dinoprost; D001205:Ascorbic Acid