Enhanced phosphorylation of a nucleolar 110-kDa protein in rat liver by dietary manipulation.
RNA polymerase 1 activity and nucleolar volume have been reported to increase in hepatocytes from rats fed a protein-free diet. Phosphorylation in vitro of a 110-kDa protein was enhanced in nuclei and nucleoli from livers of rats fed a protein-free diet. In nuclear extracts the 110-kDa protein in heat-treated nuclei was much more phosphorylated than from control liver. In contrast, casein kinase activity in the nuclear extract from control liver was comparable to that from livers of rats fed a protein-free diet. Nuclear extracts from control rat liver and livers of rats fed a protein-free diet were fractionated by DEAE-cellulose column chromatography. Casein kinase II (NII) eluted at around 0.17 M NaCl scarcely phosphorylates the 110-kDa protein. Chromatography of the nuclear extract from livers of rats fed a protein-free diet, but not from control liver, yielded fractions which eluted at 0.21-0.25 M NaCl and predominantly phosphorylated the 110-kDa protein. The phosphorylation of 110-kDa protein was not appreciably affected by a heparin concentration of 5 micrograms/ml, which completely inhibited casein kinase II. In addition, phosphorylation of the 110-kDa protein in liver nucleoli from rats fed a protein-free diet showed a lower sensitivity to heparin than that in control rat liver nucleoli. These results suggest that enhanced phosphorylation of the nuclear 110-kDa protein in livers from rats fed a protein-free diet is due to the induction of a 110-kDa protein kinase distinct from casein kinase II.
Masumi
A
A
Department of Pathological Biochemistry, Medical Research Institute, Tokyo Medical and Dental University, Japan.
Suzuki
T
T
Iijima
S
S
Tsukada
K
K
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D047388:Casein Kinases; D002467:Cell Nucleus / Q000201:enzymology / Q000378:metabolism; D002848:Chromatography, DEAE-Cellulose; D004044:Dietary Proteins / Q000378:metabolism*; D004790:Enzyme Induction; D006493:Heparin / Q000494:pharmacology; D008099:Liver / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D010770:Phosphotransferases / Q000096:biosynthesis; D011494:Protein Kinases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D004044:Dietary Proteins; D009687:Nuclear Proteins; D010761:Phosphorus Radioisotopes; D000255:Adenosine Triphosphate; D006493:Heparin; D010770:Phosphotransferases; D011494:Protein Kinases; D047388:Casein Kinases