Inhibition of taste responses to Na+ salts by epithelial Na+ channel blockers in gerbil.
The Na+ transport inhibitor amiloride blocks taste responses to NaCl by 60-70%. The purpose of the present study was to determine if greater inhibition could be achieved with three potent amiloride analogs that are specific for the epithelial Na+ channel: phenamil, 2',4'-dimethylbenzamil, and 3',4'-dichlorobenzamil. Application of phenamil (100 microM) to the anterior tongue blocked integrated responses to NaCl from the chorda tympani nerve by 98.04%, but had no significant effect on sucrose or NH4Cl. This finding suggests that the epithelial Na+ channel alone transduces the taste of NaCl in gerbil. The residual 30-40% of the response that is not blocked by amiloride can simply be explained by the fact that amiloride is less potent than phenamil. On average, 100 microM phenamil blocked responses to Na+ salts with a variety of anions by 94.2%; 100 microM 2',4'-dimethylbenzamil, by 89.83%; and 100 microM 3',4'-dichlorobenzamil, by 72.56%. Small residual responses to salts of glutamate and phosphate were not eliminated by the amiloride analogs; this suggests that other transduction mechanisms may account for a small portion of taste responses for these salts in the gerbil.
Schiffman
S S
SS
Department of Psychiatry, Duke University, Durham, NC 27706.
Suggs
M S
MS
Cragoe
E J
EJ
Erickson
R P
RP
D000584:Amiloride / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D004594:Electrophysiology; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005849:Gerbillinae; D012964:Sodium / Q000378:metabolism*; D015222:Sodium Channels / Q000187:drug effects* / Q000378:metabolism; D013649:Taste / Q000187:drug effects* / Q000502:physiology
D015222:Sodium Channels; C041470:3',4'-dichlorobenzamil; C047088:phenylamil; C053494:2',4'-dimethylbenzamil; D000584:Amiloride; D012964:Sodium