Evaluation of the effect of azapropazone on neutrophil migration in anaesthetized swine using a multichamber blister suction technique.
1. The purpose of this study was to determine the in vivo inhibitory efficacy of azapropazone on neutrophil migration. The effects of azapropazone given at a dose of 100 mg kg-1 i.v. every 2 h on the neutrophil migration into skin inflammation sites (blister fluid) as well as into an autologous serum (+/- chemoattractant) placed above the blister surface (2nd chamber) were determined. 2. Azapropazone treatment schedule maintained blood levels at 70-100 micrograms ml-1 throughout the time course (360 min) of the experiment. 3. Azapropazone significantly inhibited (48 +/- 6%) neutrophil migration into the blister fluid (as evident from the decrease in myeloperoxidase activity). 4. Azapropazone significantly inhibited the neutrophil migration into the autologous serum either with (65 +/- 5%) or (35 +/- 6%) without the chemoattractant, formyl-methionyl-leucyl-phenylalanine (FMLP). The chemoattractant, FMLP, markedly increased neutrophil migration into the autologous serum by approximately 1.5 to 2 times the non-FMLP treated group. Azapropazone was more efficacious in inhibiting the neutrophil migration in the presence of FMLP than in its absence. 5. We conclude that azapropazone is an effective inhibitor of neutrophil migration into topically inflamed sites in anaesthetized swine.
Mousa
S A
SA
E.I. Dupont de Nemours & Co., Medical Products Department, Wilmington, DE 19898.
Brown
R
R
Chan
Y
Y
Hsieh
J
J
Smith
R D
RD
D000758:Anesthesia; D000818:Animals; D001032:Apazone / Q000097:blood / Q000494:pharmacology*; D001768:Blister / Q000503:physiopathology*; D002464:Cell Migration Inhibition; D006207:Half-Life; D066298:In Vitro Techniques; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects*; D009195:Peroxidase; D012879:Skin Physiological Phenomena; D013552:Swine; D014227:Triazines / Q000494:pharmacology*
D014227:Triazines; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009195:Peroxidase; D001032:Apazone