Stereoscopic observation of enzyme distribution in lowicryl K4M-embedded specimens by conventional electron microscopy.
The present investigation was undertaken to explore the value of the Lowicryl K4M embedding technique for enzyme histochemical examination of semi-thin sections. The low-temperature embedding procedure with Lowicryl K4M was found to provide favorable conditions for preservation of enzyme activity in tissue samples. We tested the histological effects of various fixatives; the best results were obtained using 4% paraformaldehyde when testing for AcPase, AlPase, TPPase, and Mg-ATPase in the dorsal root ganglion. The three-dimensional cellular fine structure could be clearly seen in stereo pair pictures under stereoscopy.
Tomiyasu
K
K
2nd Department of Anatomy, Toho University School of Medicine, Tokyo, Japan.
Nasu
F
F
Igarashi
H
H
Inomata
K
K
D000135:Acid Phosphatase / Q000378:metabolism*; D000180:Acrylic Resins; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000166:cytology / Q000201:enzymology / Q000648:ultrastructure; D006651:Histocytochemistry / Q000379:methods; D008297:Male; D008854:Microscopy, Electron; D011755:Pyrophosphatases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013834:Thiamine Pyrophosphatase / Q000378:metabolism*
D000180:Acrylic Resins; C037883:Lowicryl 4KM resin; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D017301:Ca(2+) Mg(2+)-ATPase; D011755:Pyrophosphatases; D013834:Thiamine Pyrophosphatase