Improvement of the culture conditions for the development of human preimplantation embryos.
The culture of human preimplantation embryos from the 1-cell to the morula/blastocyst stage of development is not satisfactory at present. The success of various IVF laboratories ranges from 18 to 23%, therefore there is a requirement for improvement in the standard conditions used to culture the embryo. Using a limited number of 'spare' human embryos which were donated for research, in-vitro studies have been undertaken using various culture media. The results show that a significant improvement in viability is achieved using Ham's F-12 medium compared with other media presently used for culturing embryos.
Muggleton-Harris
A L
AL
MRC Experimental Embryology and Teratology Unit, St George's Hospital Medical School, London, UK.
Findlay
I
I
Whittingham
D G
DG
D003470:Culture Media; D046508:Culture Techniques; D047108:Embryonic Development; D005314:Embryonic and Fetal Development; D005260:Female; D006652:Histological Techniques; D006801:Humans; D011247:Pregnancy
D003470:Culture Media