A prospective comparison of DNA quantitation by image and flow cytometry.
Advances in computer and video technology suggest that image analysis may be practical method of measuring DNA that also allows visual confirmation of cell type. The purpose of this study was to prospectively compare DNA quantitation from 92 solid tumors in which DNA indices had been measured by image analysis of touch preparations (CAS 100) and flow cytometry of cell suspensions (FACScan). For 81 cases, there was excellent correlation between the two methods. For nine cases, however, an aneuploid population, usually near tetraploid, was identified by image but not by flow cytometry. Three cases had aneuploid peaks by flow cytometry that were not identified by image. Although these methods show good correlation, rare populations may be missed by CAS, presumably because of sampling errors in the touch preparation. Aneuploid populations may also be missed by flow cytometry, either because of cell loss during processing or because visual identification by image can increase sensitivity.
Bauer
T W
TW
Department of Pathology, Cleveland Clinic Foundation, Ohio 44195-5138.
Tubbs
R R
RR
Edinger
M G
MG
Suit
P F
PF
Gephardt
G N
GN
Levin
H S
HS
D000230:Adenocarcinoma / Q000032:analysis* / Q000235:genetics; D000782:Aneuploidy; D000818:Animals; D001943:Breast Neoplasms / Q000032:analysis* / Q000235:genetics; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000032:analysis* / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis*; D005069:Evaluation Studies as Topic; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007680:Kidney Neoplasms / Q000032:analysis* / Q000235:genetics; D015588:Observer Variation; D011446:Prospective Studies; D051381:Rats
D004273:DNA, Neoplasm