In-vitro activity of azithromycin against Chlamydia trachomatis.
We evaluated the efficacy of azithromycin, erythromycin and doxycycline in controlling in-vitro propagation of Chlamydia trachomatis in HeLa 229 cells. Eight recent clinical isolates of C. trachomatis and two fast-growing strains were tested with inocula of 10(3)-10(5) inclusion forming units per well of a 96-well microtitre plate. C. trachomatis inclusions were detected by an immunoperoxidase-antiperoxidase procedure (PAP), including a genus-specific monoclonal antibody. MIC ranges were 0.064-0.25 mg/l azithromycin, 0.064-0.128 mg/l erythromycin and 0.016-0.064 mg/l doxycyline; MBC ranges were 2-8 mg/l azithromycin, 4-64 mg/l erythromycin and 0.5-8 mg/l doxycycline. Since azithromycin appears to be effective against C. trachomatis, clinical studies in sexually transmitted diseases are indicated.
Scieux
C
C
Laboratory of Bacteriology and Virology, Hôpital Saint-Louis, Université Paris VII, France.
Bianchi
A
A
Chappey
B
B
Vassias
I
I
Pérol
Y
Y
D017963:Azithromycin; D002692:Chlamydia trachomatis / Q000187:drug effects*; D004318:Doxycycline / Q000494:pharmacology; D004917:Erythromycin / Q000031:analogs & derivatives* / Q000494:pharmacology; D006367:HeLa Cells
D004917:Erythromycin; D017963:Azithromycin; D004318:Doxycycline