Primary structure of the gamma subunit of the DHP-sensitive calcium channel from skeletal muscle.
Affinity-purified, polyclonal antibodies to the gamma subunit of the dihydropyridine (DHP)-sensitive, voltage-dependent calcium channel have been used to isolate complementary DNAs to the rabbit skeletal muscle protein from an expression library. The deduced primary structure indicates that the gamma subunit is a 25,058-dalton protein that contains four transmembrane domains and two N-linked glycosylation sites, consistent with biochemical analyses showing that the gamma subunit is a glycosylated hydrophobic protein. Nucleic acid hybridization studies indicate that there is a 1200-nucleotide transcript in skeletal muscle but not in brain or heart. The gamma subunit may play a role in assembly, modulation, or the structure of the skeletal muscle calcium channel.
Jay
S D
SD
Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City 52242.
Ellis
S B
SB
McCue
A F
AF
Williams
M E
ME
Vedvick
T S
TS
Harpold
M M
MM
Campbell
K P
KP
D000595:Amino Acid Sequence; D000818:Animals; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology; D004247:DNA / Q000302:isolation & purification; D004095:Dihydropyridines / Q000494:pharmacology*; D004220:Disulfides; D004591:Electrophoresis, Polyacrylamide Gel; D007118:Immunoassay; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009132:Muscles / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D011487:Protein Conformation; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D012689:Sequence Homology, Nucleic Acid
D015220:Calcium Channels; D004095:Dihydropyridines; D004220:Disulfides; D046911:Macromolecular Substances; D012333:RNA, Messenger; D004247:DNA