Human normal-resting epidermal Langerhans cells do express the type 3 complement receptor.
The expression of CR3 by murine-resting epidermal Langerhans cells (LC) is well established, but CR3 expression by human normal-resting epidermal LC has not yet been demonstrated. In this study, highly sensitive immunostaining techniques, such as immunogold labelling in transmission- and scanning-electron microscopy, were used on freshly isolated, LC-enriched, normal human epidermal cells. Human normal resting epidermal LC were found to be CR3+, since a low but significant number of gold granules labelled the plasma membrane of all the LC observed under transmission-electron microscopy, and all the epidermal cells showing LC morphology as observed by scanning-electron microscopy.
De Panfilis
G
G
Department of Dermatology, Parma University, Italy.
Soligo
D
D
Manara
G C
GC
Ferrari
C
C
Torresani
C
C
Zucchi
A
A
D002462:Cell Membrane / Q000276:immunology; D004817:Epidermis / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D007801:Langerhans Cells / Q000276:immunology*; D016177:Macrophage-1 Antigen; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D011951:Receptors, Complement / Q000032:analysis*
D016177:Macrophage-1 Antigen; D011951:Receptors, Complement