title: [A rapid method for serodiagnosis of Japanese encephalitis using persistently infected C6/J-121 cells].
abstract: The usefulness of persistently infected C6/36 (C6/J-121) cells with Japanese encephalitis virus (JEV) for a rapid serodiagnostic test was examined with the sera of men, swine and laboratory animals by indirect immunofluorescent antibody (IFA) technique. Detection of specific antibodies was completed within 3 to 5 hours. Nonspecific fluorescence frequently observed in other IFA tests was few. The sensitivity of antibody detection and the serological specificity of IFA (IgG) were similar to those of hemagglutination-inhibition (HI) test. For the detection of IgM antibodies in sera of JE-patients and naturally JEV infected swine, this method was found to be simpler and more sensitive and rapid than the conventional HI test which required 2-ME treatment of the sera.
authors.affiliation: Virsu Laboratory, Osaka Prefectural Institute of Public Health.
authors.forename: T
authors.initials: T
authors.lastname: Kimura
authors.forename: T
authors.initials: T
authors.lastname: Otake
authors.forename: N
authors.initials: N
authors.lastname: Ueba
authors.forename: Y
authors.initials: Y
authors.lastname: Minekawa
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002478:Cells, Cultured; D004664:Encephalitis Virus, Japanese / Q000276:immunology; D004672:Encephalitis, Japanese / Q000175:diagnosis* / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D011237:Predictive Value of Tests; D013552:Swine
chemical_list: D000914:Antibodies, Viral