title: In vitro analysis of the oligodendrocyte lineage in mice during demyelination and remyelination.
abstract: A demyelinating disease induced in C57B1/6N mice by intracranial injection of a coronavirus (murine hepatitis virus strain A59) is followed by functional recovery and efficient CNS myelin repair. To study the biological properties of the cells involved in this repair process, glial cells were isolated and cultured from spinal cords of these young adult mice during demyelination and remyelination. Using three-color immunofluorescence combined with [3H]thymidine autoradiography, we have analyzed the antigenic phenotype and mitotic potential of individual glial cells. We identified oligodendrocytes with an antibody to galactocerebroside, astrocytes with an antibody to glial fibrillary acidic protein, and oligodendrocyte-type 2 astrocyte (O-2A) progenitor cells with the O4 antibody. Cultures from demyelinated tissue differed in several ways from those of age-matched controls: first, the total number of O-2A lineage cells was strikingly increased; second, the O-2A population consisted of a higher proportion of O4-positive astrocytes and cells of mixed oligodendrocyte-astrocyte phenotype; and third, all the cell types within the O-2A lineage showed enhanced proliferation. This proliferation was not further enhanced by adding PDGF, basic fibroblast growth factor (bFGF), or insulin-like growth factor I (IGF-I) to the defined medium. However, bFGF and IGF-I seemed to influence the fate of O-2A lineage cells in cultures of demyelinated tissue. Basic FGF decreased the percentage of cells expressing galactocerebroside. In contrast, IGF-I increased the relative proportion of oligodendrocytes. Thus, O-2A lineage cells from adult mice display greater phenotypic plasticity and enhanced mitotic potential in response to an episode of demyelination. These properties may be linked to the efficient remyelination achieved in this demyelinating disease.
authors.affiliation: Laboratory of Viral and Molecular Pathogenesis, National Institute of Neurological Disorders and Stroke, Bethesda, Maryland 20892.
authors.forename: R
authors.initials: R
authors.lastname: Armstrong
authors.forename: V L
authors.initials: VL
authors.lastname: Friedrich
authors.forename: K V
authors.initials: KV
authors.lastname: Holmes
authors.forename: M
authors.initials: M
authors.lastname: Dubois-Dalcq
mesh_terms: D000818:Animals; D001345:Autoradiography; D002454:Cell Differentiation; D002455:Cell Division; D003333:Coronaviridae Infections / Q000473:pathology; D003711:Demyelinating Diseases / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D005455:Fluorescent Antibody Technique; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D009186:Myelin Sheath / Q000502:physiology*; D009836:Oligodendroglia / Q000166:cytology*; D010641:Phenotype; D013116:Spinal Cord / Q000166:cytology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
chemical_list: D014316:Tritium; D013936:Thymidine