Application of potassium chloride to the brain surface induces the c-fos proto-oncogene: reversal by MK-801.
The proto-oncogene c-fos is activated in the brain by a variety of stimuli including brain injury. In unilateral brain injury, c-fos immunoreactivity is confined to the damaged hemisphere, an effect reminiscent of spreading depression. Here we show that topical application of KCl (3 M) to the brain surface (which induces spreading depression) is accompanied by ipsilateral increase in c-fos immunolabeling. The activation of c-fos, like spreading depression, is markedly reduced by the non-competitive NMDA antagonist MK-801 (3 mg/kg i.p.).
Herrera
D G
DG
Department of Pharmacology, Faculty of Medicine, Dalhousie University, Halifax, N.S., Canada.
Robertson
H A
HA
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D013181:Cortical Spreading Depression / Q000502:physiology*; D003986:Dibenzocycloheptenes / Q000494:pharmacology*; D016291:Dizocilpine Maleate; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D008297:Male; D011189:Potassium Chloride / Q000494:pharmacology*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos; D051381:Rats; D011919:Rats, Inbred Strains; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000502:physiology*
D003986:Dibenzocycloheptenes; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter; D011189:Potassium Chloride; D016291:Dizocilpine Maleate