Evaluation of oral and laryngeal specimens for human papillomavirus (HPV) DNA by dot blot hybridization.
Human papillomavirus (HPV) is an infectious agent which is increasingly associated with mucosal cancers, in particular, cancer of the cervix. The present investigation was undertaken in an attempt to determine whether the Virapap human papillomavirus (HPV) DNA detection kit, originally designed for identification of HPV in exfoliated cervical cells could be employed in detection of HPV in biopsies of oral and laryngeal tissue samples, specifically oral squamous cell carcinomas, epithelial dysplasias, smokeless tobacco keratoses, and laryngeal papillomatosis. The ViraPap HPV DNA detection assay was found to be useful for HPV DNA identification in tissue samples from oral and laryngeal specimens.
Greer
R O
RO
Division of Oral Pathology and Oncology, University of Colorado School of Dentistry.
Douglas
J M
JM
Breese
P
P
Crosby
L K
LK
D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D007822:Laryngeal Neoplasms / Q000382:microbiology*; D009062:Mouth Neoplasms / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics*; D010865:Pilot Projects; D015347:RNA Probes; D015203:Reproducibility of Results
D004279:DNA, Viral; D015347:RNA Probes