Isolation of a urinary digitalis-like factor indistinguishable from digoxin.
A digitalis-like factor has been purified to apparent homogeneity from human urine based on the inhibitory effect on [3H] ouabain binding to intact human erythrocytes. The purification scheme involved large scale adsorption followed by preparative, semipreparative and analytical high-performance liquid chromatography. The purified material showed a prominent digoxin-like immunoreactivity. The behaviour of the isolated substance was identical to that of authentic digoxin in three high-performance liquid chromatography and three thin-layer chromatography systems. Moreover, fast atom bombardment mass spectrum and proton nuclear magnetic resonance spectrum suggested that the purified material may be indistinguishable from digoxin.
Goto
A
A
Second Department of Internal Medicine, University of Tokyo, Japan.
Ishiguro
T
T
Yamada
K
K
Ishii
M
M
Yoshioka
M
M
Eguchi
C
C
Shimora
M
M
Sugimoto
T
T
D001798:Blood Proteins / Q000302:isolation & purification* / Q000652:urine; D002298:Cardenolides; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004077:Digoxin / Q000302:isolation & purification* / Q000652:urine; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010042:Ouabain / Q000378:metabolism; D012503:Saponins; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*; D014316:Tritium
D001798:Blood Proteins; D002298:Cardenolides; D012503:Saponins; C034504:digoxin-like factors; D014316:Tritium; D010042:Ouabain; D004077:Digoxin; D000254:Sodium-Potassium-Exchanging ATPase