Regulation of thiamine biosynthesis in Saccharomyces cerevisiae.
A pho6 mutant of Saccharomyces cerevisiae, lacking a regulatory gene for the synthesis of periplasmic thiamine-repressible acid phosphatase activity, was found to be auxotrophic for thiamine. The activities of four enzymes involved in the synthesis of thiamine monophosphate were hardly detectable in the crude extract from the pho6 mutant. On the other hand, the activities of these enzymes and thiamine-repressible acid phosphatase in a wild-type strain of S. cerevisiae, H42, decreased with the increase in the concentration of thiamine in yeast cells. These results suggest that thiamine synthesis in S. cerevisiae is subject to a positive regulatory gene, PHO6, whereas it is controlled negatively by the intracellular thiamine level.
Kawasaki
Y
Y
Department of Biochemistry, Kyoto Prefectural University of Medicine, Japan.
Nosaka
K
K
Kaneko
Y
Y
Nishimura
H
H
Iwashima
A
A
D000135:Acid Phosphatase / Q000235:genetics; D019883:Alkyl and Aryl Transferases; D005800:Genes, Fungal; D005809:Genes, Regulator; D007700:Kinetics; D009154:Mutation; D010770:Phosphotransferases / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor); D017856:Phosphotransferases (Phosphate Group Acceptor); D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D013831:Thiamine / Q000096:biosynthesis*; D014166:Transferases / Q000378:metabolism
D014166:Transferases; D019883:Alkyl and Aryl Transferases; C435495:thiamin phosphate synthase; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C059599:hydroxymethylpyrimidine kinase; C051254:hydroxyethylthiazole kinase; D017856:Phosphotransferases (Phosphate Group Acceptor); C033532:phosphomethylpyrimidine kinase; D000135:Acid Phosphatase; D013831:Thiamine