In vivo and in vitro collagenolytic activity of Acanthamoeba castellanii.
Axenic cultures of Acanthamoeba castellanii contained a collagenolytic enzyme that digested collagen shields and purified collagen in vitro. Specificity of biologic activity was determined by the addition of selected enzyme inhibitors to the assays and revealed that the parasite-conditioned medium contained both collagenase and lower concentrations of other proteolytic enzymes. However, most of the collagenolytic and pathogenic activity was directly attributable to specific collagenase. Intrastromal injection of sterile, Acanthamoeba-conditioned culture medium into naive Lewis rats produced corneal lesions clinically similar to and closely resembling those found in biopsy specimens of human patients diagnosed with acanthamoebic keratitis. Histopathologic analysis revealed moderate-to-severe neutrophil infiltration, disruption of stromal lamellae, and edema. Identical pathologic sequelae were produced by intrastromal injection of purified collagenase (25 units/ml). The pathogenicity of the soluble parasite-derived product was removed by passage over affinity columns armed with antibody specific for collagenase. These results indicated that soluble parasite-derived factors were capable of producing lesions characteristic of acanthamoebic keratitis and that the pathogenicity of these factors was either directly or indirectly attributable to specific collagenase activity.
He
Y G
YG
Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas 75235.
Niederkorn
J Y
JY
McCulley
J P
JP
Stewart
G L
GL
Meyer
D R
DR
Silvany
R
R
Dougherty
J
J
D000048:Acanthamoeba / Q000201:enzymology*; D015823:Acanthamoeba Keratitis / Q000201:enzymology / Q000473:pathology; D000818:Animals; D001684:Biological Dressings; D002846:Chromatography, Affinity; D003094:Collagen / Q000378:metabolism*; D003319:Corneal Stroma / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D003012:Microbial Collagenase / Q000008:administration & dosage / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew
D003094:Collagen; D003012:Microbial Collagenase