Isolation of anti-endothelin receptor monoclonal antibodies for use in receptor characterization.
Monoclonal antibodies reactive with endothelin (ET) receptors have been prepared by immunization of mice with rat lung membranes. Of four clones isolated, three clones preferentially recognized 32,000-dalton ET receptor and the other has a higher affinity for the 45,000-dalton receptor. The binding of 125I-ET-1 to detergent-solubilized ET receptors which were adsorbed to the antibodies was displaced by increasing concentrations of unlabeled ET isopeptides. These results demonstrate that the four clones specific for the receptor have the potential to be a useful tool in the characterization of ET receptors.
Kondoh
M
M
Institute of Applied Biochemistry, Gene Experiment Center, Ibaraki, Japan.
Miyazaki
H
H
Watanabe
H
H
Shibata
T
T
Yanagisawa
M
M
Masaki
T
T
Murakami
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D001667:Binding, Competitive; D002462:Cell Membrane / Q000378:metabolism; D016232:Endothelins / Q000378:metabolism; D007700:Kinetics; D008168:Lung / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008970:Molecular Weight; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D017466:Receptors, Endothelin
D000911:Antibodies, Monoclonal; D016232:Endothelins; D011956:Receptors, Cell Surface; D017466:Receptors, Endothelin