A novel RNA helicase gene tightly linked to the Triplo-lethal locus of Drosophila.
The Triplo-lethal (Tpl) locus of Drosophila is the only known locus which is lethal when present in three copies rather than the normal two. After recovering a hybrid-dysgenesis-induced mutation of Tpl we used a rapid combination of transposon tagging, chromosome microdissection and PCR to clone the P element that had transposed into the Tpl region. That P element is located within the gene for a new and unique member of the RNA helicase family. This new helicase differs from all others known by having glycine-rich repeats at both the amino and carboxyl termini. Curiously, genetic analysis shows that the P element inserted into this gene is not responsible for the Tpl mutant phenotype. We present possible explanations for these findings.
Dorer
D R
DR
Department of Biochemistry and Molecular Biology, Jefferson Institute of Molecular Medicine, Thomas Jefferson University, Philadelphia, PA 19107.
Christensen
A C
AC
Johnson
D H
DH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004251:DNA Transposable Elements; D004330:Drosophila / Q000201:enzymology / Q000235:genetics*; D005091:Exons; D005796:Genes; D008040:Genetic Linkage; D007438:Introns; D008969:Molecular Sequence Data; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012316:RNA Nucleotidyltransferases / Q000235:genetics*; D015183:Restriction Mapping; D014158:Transcription, Genetic
D004251:DNA Transposable Elements; D004247:DNA; D012316:RNA Nucleotidyltransferases