title: Partial purification and characterization of trehalase from axenically grown myxamoebae of Dictyostelium discoideum.
abstract: Lysosomal trehalase from the myxamoebae of Dictyostelium discoideum has been partially purified. The behavior of the enzyme under different chromatographic and electrophoretic conditions reveals its close similarities to other lysosomal enzymes that have been studied earlier. The cellular trehalase, which is electrophoretically homogeneous, appears as two peaks of activity when subjected to hydroxyapatite and gel filtration chromatography. The enzyme has isoelectric points of 4.0 and less than 2.5. Among natural disaccharides tested, the purified trehalase showed absolute specificity for trehalose with an apparent Km of 1.15 mM. However, the enzyme efficiently utilized the synthetic sugar alpha-D-glucosyl fluoride as a substrate. Various methods were employed to estimate the apparent molecular weight, which was found to lie in the range of 30-162 kDa.
authors.affiliation: Department of Biological Sciences, University of Windsor, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Gupta
authors.forename: D A
authors.initials: DA
authors.lastname: Cotter
mesh_terms: D002845:Chromatography; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004023:Dictyostelium / Q000201:enzymology* / Q000254:growth & development; D017886:Durapatite; D004591:Electrophoresis, Polyacrylamide Gel; D006882:Hydroxyapatites; D007700:Kinetics; D008970:Molecular Weight; D014198:Trehalase / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D006882:Hydroxyapatites; D017886:Durapatite; D014198:Trehalase