Mouse autoantibodies bind to a phospholipase-C-sensitive structure on red blood cells.
After culturing mouse peritoneal cells in vitro for 4 days, high numbers of cells can be detected that secrete autoantibodies against isologous red blood cells (RBC), modified with the proteolytic enzyme bromelain (Brom). Plaque-forming cell numbers against mouse Brom RBC were significantly reduced by pretreating mouse Brom RBC prior to haemolytic assay with phospholipase C, an enzyme that hydrolyzes phospholipids, notably phosphatidylcholine. In contrast, further treatment of mouse Brom RBC with Brom, neuraminidase, beta-chymotrypsin, trypsin, or papain had no effect on plaque-forming cell numbers. These results show that phosphatidylcholine is an integral part of the mouse RBC autoantigen exposed by Brom treatment.
Hardy
S J
SJ
School of Biological Sciences, Flinders University of South Australia, Bedford Park.
Cox
K O
KO
D000818:Animals; D001323:Autoantibodies / Q000378:metabolism*; D001324:Autoantigens / Q000378:metabolism; D001666:Binding Sites, Antibody; D001963:Bromelains; D004912:Erythrocytes / Q000378:metabolism*; D006462:Hemolytic Plaque Technique; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D010529:Peritoneal Cavity; D010713:Phosphatidylcholines / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism*
D001323:Autoantibodies; D001324:Autoantigens; D010713:Phosphatidylcholines; D001963:Bromelains; D010738:Type C Phospholipases