A neutralizing monoclonal antibody against Coxsackievirus B4 cross-reacts with contractile muscle proteins.
A panel of Coxsackievirus B4 (CVB4) neutralizing monoclonal antibodies (mAbs) were tested against a panel of normal mouse tissues. One mAb, 356-1, reacted specifically with murine heart tissue. Immunohistochemical studies revealed an A band pattern of staining of the heart. Examination of sequential differential extracts of heart by Western immunoblotting showed that 356-1 predominantly reacted with the murine cardiac myosin heavy chain. A rather weak cross-reaction was found with actin. These observations were confirmed by the binding of 356-1 to purified cardiac myosin and actin. This antibody showed a higher affinity for murine cardiac muscle myosin than for skeletal muscle myosin. Examination of the reactivity of 356-1 with CVB4 polypeptides using Western immunoblotting revealed that 356-1 binds to the VP-1 capsid protein. These studies imply that molecular mimicry is one mechanism by which autoimmunity could develop during CVB4 induced myocarditis.
Beisel
K W
KW
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198.
Srinivasappa
J
J
Olsen
M R
MR
Stiff
A C
AC
Essani
K
K
Prabhakar
B S
BS
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000276:immunology*; D002213:Capsid / Q000276:immunology; D003429:Cross Reactions; D029822:Enterovirus B, Human / Q000276:immunology*; D051379:Mice; D009206:Myocardium / Q000276:immunology; D009218:Myosins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D009218:Myosins