Upstream activation and repression elements control transcription of the yeast COX5b gene.
The Saccharomyces cerevisiae COX5b gene is regulated at the level of transcription by both the carbon source and oxygen. To define the cis-acting elements that underlie this transcriptional control, deletion analysis of the upstream regulatory region of COX5b was performed. The results of the study suggest that at least four distinct regulatory sites are functional upstream of the COX5b transcriptional starts. One, which was precisely defined to a region of 20 base pairs, contains two TATA-like elements. Two upstream activating sequences (UAS15b and UAS2(5b)) and an upstream repression sequence (URS5b) were also found. Each of the latter three elements was able either to activate (UAS1(5b) and UAS2(5b)) or to repress URS5b) the transcription of a heterologous yeast gene. Further analysis revealed that UAS1(5b) is the site of carbon source control and may be composed of two distinct domains that act synergistically. URS5b mediates the aerobic repression of COX5b and contains two sequences that are highly conserved in other yeast genes negatively regulated by oxygen.
Hodge
M R
MR
Department of Molecular Biology and Biochemistry, University of California, Irvine 92717.
Singh
K
K
Cumsky
M G
MG
D001483:Base Sequence; D004252:DNA Mutational Analysis; D004261:DNA Replication; D003576:Electron Transport Complex IV / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D005800:Genes, Fungal; D005947:Glucose / Q000502:physiology; D006418:Heme / Q000502:physiology; D008969:Molecular Sequence Data; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic
D006418:Heme; D003576:Electron Transport Complex IV; D005947:Glucose