Immunological relationship between feline herpesvirus type 1 (FHV-1) and canine herpesvirus (CHV) as revealed by polyvalent and monoclonal antibodies.
Polyvalent and monoclonal antibodies were used to demonstrate serological and antigenic relationships between feline herpesvirus type 1 (FHV-1) and canine herpesvirus (CHV). Using virus neutralization tests, enzyme-linked immunosorbent and indirect immunofluorescence assays, and immunoblotting analysis, reciprocal cross-reactivities to the heterologous virus were observed with some polyvalent and monoclonal antibodies. One monoclonal antibody against CHV neutralized FHV-1 infectivity and one monoclonal antibody against FHV-1 inhibited the hemagglutination activity of CHV as well as FHV-1-infected mouse serum. The major cross-reacting proteins were identified as the 143/108 kDa and 60 kDa glycoproteins of FHV-1 and the 145/112 kDa and 41 kDa glycoproteins of CHV. Previously, we have identified the 60 kDa and 41 kDa glycoproteins as the hemagglutinins of FHV-1 and CHV, respectively. The present results indicated the presence of shared antigenic determinants among these proteins.
Limcumpao
J A
JA
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Horimoto
T
T
Xuan
X
X
Takahashi
E
E
Mikami
T
T
D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006385:Hemagglutination Inhibition Tests; D006564:Herpesviridae / Q000145:classification / Q000276:immunology*; D002184:Herpesvirus 1, Canid / Q000145:classification / Q000276:immunology*; D015151:Immunoblotting; D009500:Neutralization Tests
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral