title: Amino acid sequence of bovine osteoinductive factor.
abstract: The complete amino acid sequence of bovine osteoinductive factor (OIF) was determined by automated Edman degradation of S-pyridylethylated bovine OIF and selected fragments. Cleavage with endoproteinase Lys-C, endoproteinase Glu-C, or endoproteinase Asp-N established all fragments in an unambiguous sequence. Bovine OIF contains 105 residues with a calculated molecular weight of 12,055. It is a single chain polypeptide containing two intramolecularly linked cysteines at residues 62 and 95. Two asparagine-linked glycosylation sites at positions 52 and 65 were found by comparing sequence data and peptide profiles of native and deglycosylated OIF fragments. The amino acid sequence of OIF has no homology to other reported proteins.
authors.affiliation: Celtrix Laboratories, Collagen Corporation, Palo Alto, California 94303.
authors.forename: H
authors.initials: H
authors.lastname: Bentz
authors.forename: R J
authors.initials: RJ
authors.lastname: Chang
authors.forename: A Y
authors.initials: AY
authors.lastname: Thompson
authors.forename: C B
authors.initials: CB
authors.lastname: Glaser
authors.forename: D M
authors.initials: DM
authors.lastname: Rosen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001842:Bone and Bones / Q000378:metabolism*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D006023:Glycoproteins / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D010447:Peptide Hydrolases; D011487:Protein Conformation
chemical_list: D006023:Glycoproteins; D010446:Peptide Fragments; D010447:Peptide Hydrolases