Sequence analysis of the gene (6) encoding the major capsid protein (VP6) of group C rotavirus: higher than expected homology to the corresponding protein from group A virus.
Two overlapping c-DNA clones, which hybridized in Northern blots to RNA segment 6 of the prototype strain (Cowden) of group C rotavirus, were selected from a c-DNA library in pBR322 and sequenced. The gene 6 sequence obtained was 1349 nucleotides and contained a single long open reading frame encoding a protein of 394 amino acids (total MW, 44,479) which is in line with the size of the major capsid protein VP6. Comparison of the group C sequence with that of the corresponding group A rotavirus gene revealed homology levels of 55 and 42% for nucleotides and amino acids, respectively. These values were surprisingly high in view of previous immunological and nucleic acid hybridization data which failed to show any cross-reaction between group A and non-group A rotaviruses. The epidemiological implications of these observations are discussed.
Bremont
M
M
Laboratoire de Virologie et d'Immunologie Moléculaires INRA, C.R.J. Domaine de Vilvert, Jouy-en-Josas, France.
Chabanne-Vautherot
D
D
Vannier
P
P
McCrae
M A
MA
Cohen
J
J
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000276:immunology; D001483:Base Sequence; D002213:Capsid / Q000235:genetics* / Q000276:immunology; D036022:Capsid Proteins; D003001:Cloning, Molecular; D003429:Cross Reactions; D004279:DNA, Viral / Q000737:chemistry; D005814:Genes, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012401:Rotavirus / Q000235:genetics* / Q000276:immunology; D012689:Sequence Homology, Nucleic Acid
D000956:Antigens, Viral; D036022:Capsid Proteins; D004279:DNA, Viral; C050779:VP7 protein, Rotavirus