title: Expression of guanine nucleotide binding proteins, Gs and Gi, in mRNAs in epidermal keratinocytes.
abstract: The stimulation or inhibition of adenylate cyclase by hormones and chemicals is mediated by the stimulatory or inhibitory guanine nucleotide-binding proteins, Gs and Gi respectively. Although the presence of these G-proteins in the epidermis has been suggested, no direct information regarding their nature has been available. Using cDNAs for these G-proteins as a probe, we investigated the nature of the G-proteins of various keratinocytes. FRSK cells, a cell line derived from fetal rat epidermal cells, and SV-40-transformed human epidermal cells, both of which are highly proliferative keratinocytes, contained mRNAs for both Gs-alpha and two Gi-alpha proteins (Gi-2 alpha and -3 alpha). No evidence for the presence of Gi-1 alpha or Go-alpha was obtained. Normal human or pig epidermis had a relatively small amount of mRNAs for these G-proteins in the stable (hypoproliferative) condition. Tape-stripping-induced, and UVB-induced hyperproliferative epidermis contained an increased amount of these G-protein messages. However, all Gs-alpha, Gi-2 alpha and -3 alpha, as well as beta-actin mRNAs, were increased to a similar extent, and no specific expression of G-protein messages could be detected in the hyperproliferative epidermis.
authors.affiliation: Department of Dermatology, Asahikawa Medical College, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: N
authors.initials: N
authors.lastname: Miyokawa
authors.forename: M
authors.initials: M
authors.lastname: Katagiri
authors.forename: H
authors.initials: H
authors.lastname: Iizuka
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D002461:Cell Line, Transformed; D015342:DNA Probes; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D013539:Simian virus 40 / Q000502:physiology; D013552:Swine; D014466:Ultraviolet Rays
chemical_list: D015342:DNA Probes; D012333:RNA, Messenger; D019204:GTP-Binding Proteins