title: Metals inhibit riboflavin-catalyzed generation of superoxide anion in vitro.
abstract: We found that addition of cationic metals inhibited flavin-catalyzed superoxide anion (O2-.) production in vitro. Inhibition of O2-. generation by metals appeared to relate to the ability of metal ions to chelate or complex with amine groups, altering their electronegativity. Metal inhibition of O2-. production has important implications for biological systems involving O2-. radical production, as well as for assays requiring the generation of O2-. in vitro.
authors.affiliation: Department of Medicine, University of Colorado Health Sciences Center, Denver.
authors.forename: L S
authors.initials: LS
authors.lastname: Terada
authors.forename: J A
authors.initials: JA
authors.lastname: Leff
authors.forename: D N
authors.initials: DN
authors.lastname: Guidot
authors.forename: G A
authors.initials: GA
authors.lastname: Shibao
authors.forename: J E
authors.initials: JE
authors.lastname: Repine
mesh_terms: D002384:Catalysis; D004492:Edetic Acid; D066298:In Vitro Techniques; D008670:Metals; D008715:Methionine; D009538:Nicotine; D012256:Riboflavin; D013481:Superoxides
chemical_list: D008670:Metals; D013481:Superoxides; D009538:Nicotine; D004492:Edetic Acid; D008715:Methionine; D012256:Riboflavin