title: Evidence for posttranscriptional regulation of synthesis of the Bacillus subtilis Gnt repressor.
abstract: Transcription of the Bacillus subtilis gnt operon results in a polycistronic mRNA that encodes from the 5' end the Gnt repressor, gluconate kinase and permease. The RNA is drastically induced through inactivation of the Gnt repressor by gluconate. The results of deletion analysis of the gnt promoter region upstream of the repressor gene indicated that no other promoter except the gnt promoter was present for expression of the gluconate kinase gene. In contrast to the synthesis of gluconate kinase and permease, which was markedly induced by gluconate, the results of a radioimmunoassay for the Gnt repressor indicated that synthesis of the Gnt repressor from the induced mRNA was posttranscriptionally repressed.
authors.affiliation: Department of Biotechnology, Faculty of Engineering, Fukuyama University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Fujita
authors.forename: T
authors.initials: T
authors.lastname: Fujita
authors.forename: Y
authors.initials: Y
authors.lastname: Miwa
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D002872:Chromosome Deletion; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D005942:Gluconates / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010770:Phosphotransferases / Q000235:genetics* / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor); D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000032:analysis; D011863:Radioimmunoassay; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D029968:Escherichia coli Proteins; D005942:Gluconates; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; D014157:Transcription Factors; C104633:gluconate permease, E coli; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C020578:gluconokinase; C030691:gluconic acid