title: Cytoplasmic acidification and activation of Na+/H+ exchange during regulatory volume decrease in Ehrlich ascites tumor cells.
abstract: Ehrlich ascites tumor cells undergoing regulatory volume decrease (RVD) exhibit cytoplasmic acidification as measured by an intracellular fluorescent pH indicator. The acidification results in an activation of the Na+/H+ exchanger. The intracellular pH 'set point' for the activation is estimated to be around 7.0. The activation of the Na+H+ exchanger leads to an incomplete RVD. In support of this conclusion, amiloride and Na+-free medium, known to limit the Na+/H+ exchange, indeed enhance the RVD response. Intracellular acidification and activation of Na+/H+ exchange may be a general response of cells undergoing RVD.
authors.affiliation: Institute of Biological Chemistry, University of Copenhagen, Denmark.
authors.forename: A
authors.initials: A
authors.lastname: Livne
authors.forename: E K
authors.initials: EK
authors.lastname: Hoffmann
mesh_terms: D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism* / Q000473:pathology; D002352:Carrier Proteins / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007474:Ion Exchange; D051379:Mice; D009994:Osmolar Concentration; D011522:Protons; D012964:Sodium / Q000378:metabolism*; D017923:Sodium-Hydrogen Exchangers; D014407:Tumor Cells, Cultured
chemical_list: D002352:Carrier Proteins; D011522:Protons; D017923:Sodium-Hydrogen Exchangers; D000584:Amiloride; D012964:Sodium