Recognition of N-glycosidic carbohydrates on esophageal carcinoma cells by macrophage cell line THP-1.
Cell-to-cell contact between macrophages and tumor cells is an important initial reaction in a host defense mechanism against tumor cells. The authors have studied cell surface components of human esophageal carcinoma cells recognized by macrophages. Superoxide release from THP-1 cells, a human macrophage cell line, was analyzed in their interaction with a battery of human squamous cell carcinoma cell lines (TE) originated from esophageal cancer patients. The macrophage-triggering ability of TE 1 cell line, a high stimulant, was reduced after treatment with trypsin or tunicamycin, an inhibitor of N-glycosidic glycosylation. Addition of monosaccharides was efficient in competitive inhibition of these cellular interaction. Moreover, con-A-resistant mutation of TE 1 cells was found to reduce their macrophage-triggering ability, associated with increase of L-PHA-binding capacity, suggesting substitution to the GlcNAc beta(1----6)-linked lactosamine antenna in N-glycosidic carbohydrates. These findings suggest that terminal residues of N-glycosidic carbohydrates on some esophageal carcinoma cells may contribute to the recognition sites of macrophages.
Takano
R
R
Department of Pathology, Tohoku University School of Medicine, Sendai, Japan.
Nose
M
M
Kanno
H
H
Nishihira
T
T
Hiraizumi
S
S
Kobata
A
A
Kyogoku
M
M
D002294:Carcinoma, Squamous Cell / Q000032:analysis / Q000378:metabolism / Q000473:pathology*; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D002462:Cell Membrane / Q000032:analysis; D003208:Concanavalin A / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004938:Esophageal Neoplasms / Q000032:analysis / Q000378:metabolism / Q000473:pathology*; D006027:Glycosides / Q000032:analysis*; D006801:Humans; D008264:Macrophages / Q000032:analysis / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D009005:Monosaccharides / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000032:analysis / Q000187:drug effects / Q000473:pathology; D014415:Tunicamycin / Q000494:pharmacology
D006027:Glycosides; D009005:Monosaccharides; D010835:Phytohemagglutinins; D003208:Concanavalin A; D013481:Superoxides; D014415:Tunicamycin; D014357:Trypsin