title: The influence of barley fibre on bile composition, gallstone formation, serum cholesterol and intestinal morphology in hamsters.
abstract: Frequency of gallstones, concentration of bile acids and cholesterol in bile, concentration of cholesterol in serum, and structure of the small intestinal mucosa were analyzed in male Syrian Golden hamsters fed a stone provoking fibre-free diet with or without supplementation of brewer's spent grain (BSG), a concentrated barley fibre source from the by-product of brewing. A significantly lower frequency of gallstones was found in the animals with 10% BSG dietary supplementation. Addition of 30% BSG after an initial 6-week period with a fibre-free, stone provoking diet seemed to dissolve previously formed gallstones. Total bile acid concentration was higher in bile from animals given a diet supplemented with 10% BSG. In addition, the cholesterol concentration in both serum and bile was lower in the 30% BSG supplemented group. Structurally, a 10% BSG supplementation decreased ileal epithelium height whereas a high supplementation (30%) of BSG induced a decrease in epithelial height both of jejunal and ileal mucosa. The results show that BSG has significant effects on the metabolism of bile acids and cholesterol as well as on the morphology of the small intestinal mucosa.
authors.affiliation: Department of Pathology and Nutritional Research, University of Umeå, Sweden.
authors.forename: J X
authors.initials: JX
authors.lastname: Zhang
authors.forename: F
authors.initials: F
authors.lastname: Bergman
authors.forename: G
authors.initials: G
authors.lastname: Hallmans
authors.forename: G
authors.initials: G
authors.lastname: Johansson
authors.forename: E
authors.initials: E
authors.lastname: Lundin
authors.forename: R
authors.initials: R
authors.lastname: Stenling
authors.forename: O
authors.initials: O
authors.lastname: Theander
authors.forename: E
authors.initials: E
authors.lastname: Westerlund
mesh_terms: D000818:Animals; D001646:Bile / Q000032:analysis*; D002769:Cholelithiasis / Q000209:etiology / Q000517:prevention & control*; D002784:Cholesterol / Q000097:blood*; D006224:Cricetinae; D004043:Dietary Fiber / Q000494:pharmacology*; D001467:Hordeum; D007413:Intestinal Mucosa / Q000166:cytology; D007421:Intestine, Small / Q000166:cytology*; D008297:Male; D008647:Mesocricetus
chemical_list: D004043:Dietary Fiber; D002784:Cholesterol