title: The effect of the gamma-subunit of the cyclic GMP phosphodiesterase of bovine and frog (Rana catesbiana) retinal rod outer segments on the kinetic parameters of the enzyme.
abstract: Rod-outer-segment cyclic GMP phosphodiesterase (PDE) (subunit composition alpha beta gamma 2) contains catalytic activity in alpha beta. The gamma-subunits are inhibitors. Removal of the gamma-subunits increases Vmax. without affecting the Km. The inhibitory effect of a single gamma-subunit (alpha beta gamma) on the Vmax. of alpha beta is much greater in bovine than in frog (Rana catesbiana) PDE. Bovine PDE in the alpha beta gamma 2 state has a Vmax. that is 2.6 +/- 0.4% of the Vmax. of alpha beta. The removal of one gamma-subunit to give alpha beta gamma results in a Vmax. 5.2 +/- 1% of that for maximal activity. Frog alpha beta gamma 2 has a Vmax. 10.8 +/- 2%, and alpha beta gamma has a Vmax. 50 +/- 18%, of the Vmax. of alpha beta. These data suggest that a single gamma-subunit can inhibit the catalytic activity of active sites on both alpha- and beta-subunits in bovine, but not in frog, rod-outer-segment PDE.
authors.affiliation: Life Sciences Division, Los Alamos National Laboratory, NM 87545.
authors.forename: M M
authors.initials: MM
authors.lastname: Whalen
authors.forename: M W
authors.initials: MW
authors.lastname: Bitensky
authors.forename: D J
authors.initials: DJ
authors.lastname: Takemoto
mesh_terms: D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D007700:Kinetics; D046911:Macromolecular Substances; D010786:Photoreceptor Cells / Q000201:enzymology*; D011863:Radioimmunoassay; D011892:Rana catesbeiana; D012374:Rod Cell Outer Segment / Q000201:enzymology*
chemical_list: D046911:Macromolecular Substances; D015106:3',5'-Cyclic-GMP Phosphodiesterases