Aspartokinase II from Bacillus subtilis is degraded in response to nutrient limitation.
Aspartokinase II from Bacillus subtilis was shown by immunochemical methods to be regulated by degradation in response to starvation of cells for various nutrients. Ammonium starvation induced the fastest aspartokinase II decline (t1/2 = 65 min), followed by amino acid starvation (t1/2 = 80 min) and glucose limitation (t1/2 = 120 min). Loss of enzyme activity was closely correlated with the disappearance of the alpha subunit; degradation of the beta subunit was somewhat delayed or slower under some conditions. Pulse-chase experiments demonstrated that aspartokinase II was stable during exponential growth; the synthesis of the enzyme rapidly declined in response to nutrient exhaustion. The degradation of aspartokinase II was interrupted by inhibitors of energy production and protein synthesis but was not changed in a mutant lacking a major intracellular protease. Mutants lacking a normal stringent response displayed only a slight decrease in the rate of aspartokinase II degradation, even though aspartate transcarbamylase was degraded more slowly in the same mutant cells. These results indicate that although energy-dependent degradation of biosynthetic enzymes is a general phenomenon in nutrient-starved B. subtilis cells, the degradation of specific enzymes probably involves different pathways.
Graves
L M
LM
Department of Biochemistry, University of Illinois, Urbana 61801.
Switzer
R L
RL
D000595:Amino Acid Sequence; D000641:Ammonia / Q000494:pharmacology; D001222:Aspartate Kinase / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D001412:Bacillus subtilis / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D002701:Chloramphenicol / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D007527:Isoenzymes / Q000096:biosynthesis / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D010770:Phosphotransferases / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid
D007527:Isoenzymes; D002701:Chloramphenicol; D000641:Ammonia; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D010770:Phosphotransferases; D001222:Aspartate Kinase; D005947:Glucose