Involvement of pertussis toxin-sensitive GTP-binding protein in prostaglandin F2 alpha- induced phosphoinositide hydrolysis in osteoblast-like cells.
Prostaglandin F2 alpha (PGF2 alpha) stimulated the formation of inositol phosphates in a dose-dependent manner in cloned osteoblast-like MC3T3-E1 cells. This reaction was markedly inhibited dose-dependently by pertussis toxin. In the cell membranes, pertussis toxin-catalyzed ADP-ribosylation of a 40-kDa protein was significantly attenuated by pretreatment of PGF2 alpha. These results suggest that pertussis toxin-sensitive GTP-binding protein is involved in the coupling of PGF2 alpha receptor to phospholipase C in these cells.
Miwa
M
M
First Department of Internal Medicine, Nagoya University School of Medicine, Japan.
Tokuda
H
H
Tsushita
K
K
Kotoyori
J
J
Takahashi
Y
Y
Ozaki
N
N
Kozawa
O
O
Oiso
Y
Y
D000818:Animals; D002460:Cell Line; D015237:Dinoprost / Q000494:pharmacology*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006868:Hydrolysis; D007295:Inositol Phosphates / Q000378:metabolism; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism; D010006:Osteoblasts; D037342:Pertussis Toxin; D010716:Phosphatidylinositols / Q000378:metabolism*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D007295:Inositol Phosphates; D008565:Membrane Proteins; D010716:Phosphatidylinositols; D010566:Virulence Factors, Bordetella; D015237:Dinoprost; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins