title: Characteristics of atrial natriuretic hormone receptors in human pheochromocytomas.
abstract: The presence of functional receptors for human atrial natriuretic hormone in human pheochromocytomas was recently reported. The present study reports the binding of hANH as measured by Scatchard analysis in 4 human adrenal glands and in 5 human pheochromocytomas. Binding assays using [3H]ANH revealed a single class of high-affinity binding sites for hANH in both tissues. Human pheochromocytomas present a lower number of binding sites than normal human adrenal gland (Bmax of 7.1 +/- 2.1 vs 33.6 +/- 6.9 fmol/mg protein, respectively). However, the decreased number of ANH receptors was not paralleled by modifications of tissular cyclic GMP (cGMP). Moreover, plasma hANH concentrations in 7 patients with pheochromocytomas (20.2 +/- 2.7 pmol/l) were statistically higher than those obtained in 25 normal control humans (8.1 +/- 0.6 pmol/l, p less than 0.001). We also demonstrated the presence of immunoreactive ANH in the tumour itself.
authors.affiliation: Service de Biochimie, Faculté de médecine Pitié-Salpêtrière, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Eurin
authors.forename: A
authors.initials: A
authors.lastname: Carayon
authors.forename: M A
authors.initials: MA
authors.lastname: Zongazo
authors.forename: F
authors.initials: F
authors.lastname: Masson
authors.forename: C
authors.initials: C
authors.lastname: Barthelemy
authors.forename: G
authors.initials: G
authors.lastname: Maistre
authors.forename: J J
authors.initials: JJ
authors.lastname: Rouby
authors.forename: B
authors.initials: B
authors.lastname: Eurin
authors.forename: J C
authors.initials: JC
authors.lastname: Legrand
mesh_terms: D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000311:Adrenal Glands / Q000378:metabolism; D000450:Aldosterone / Q000378:metabolism; D000804:Angiotensin II / Q000378:metabolism; D009320:Atrial Natriuretic Factor / Q000378:metabolism; D001667:Binding, Competitive; D002852:Chromatography, Ion Exchange; D006152:Cyclic GMP / Q000032:analysis; D003900:Desoxycorticosterone / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D006801:Humans; D009478:Neuropeptide Y / Q000378:metabolism; D010673:Pheochromocytoma / Q000378:metabolism*; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000378:metabolism; D014667:Vasopressins / Q000378:metabolism
chemical_list: D009478:Neuropeptide Y; D011956:Receptors, Cell Surface; D014667:Vasopressins; D000804:Angiotensin II; D003900:Desoxycorticosterone; D000450:Aldosterone; D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor; D006152:Cyclic GMP; D004298:Dopamine