Okadaic acid uncouples myosin light chain phosphorylation and tension in smooth muscle.
Tracheal smooth muscle precontracted with carbachol relaxes upon the addition of 3 microM okadaic acid. Although cytosolic Ca2+ concentrations decrease, myosin light chain remains highly phosphorylated (50%). In smooth muscle treated with carbachol alone or carbachol plus okadaic acid 32P is incorporated into a single peptide on myosin light chain which corresponds to the site phosphorylated by myosin light chain kinase. Treatment with okadaic acid alone does not result in myosin light chain phosphorylation or tension development. These results suggest that a cellular mechanism other than myosin light chain phosphorylation can regulate contractile tension.
Tansey
M G
MG
Department of Physiology, University of Texas Southwestern Medical Center, Dallas 75235.
Hori
M
M
Karaki
H
H
Kamm
K E
KE
Stull
J T
JT
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002217:Carbachol / Q000494:pharmacology; D002417:Cattle; D004988:Ethers, Cyclic / Q000494:pharmacology*; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects* / Q000378:metabolism; D009218:Myosins / Q000378:metabolism*; D019319:Okadaic Acid; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors*; D010750:Phosphoproteins / Q000502:physiology; D010766:Phosphorylation; D014132:Trachea / Q000187:drug effects
D004988:Ethers, Cyclic; D010750:Phosphoproteins; D019319:Okadaic Acid; D002217:Carbachol; D010749:Phosphoprotein Phosphatases; D009218:Myosins; D002118:Calcium