Phenotypic variation in human HepG2 hepatoma cells: alterations in cell growth, plasma protein synthesis and heme pathway enzymes.
1. Growth rates, morphology, plasma protein synthesis and the level of heme pathway enzymes were examined in six sublines of HepG2 cells obtained from various laboratories. 2. Five sublines represented by G2a display the known characteristics of HepG2 cell type, including morphology, plasma protein synthesis and an increase in delta-aminolevulinic acid (ALA) dehydratase activities in response to Me2SO treatment. 3. In contrast, cells of the G2f subline failed to secrete significant quantities of plasma proteins. There was also no increase in ALA dehydratase activity following Me2SO treatment. These findings suggest that G2f cells represent a variant of HepG2 cells with an altered phenotype.
Iwasa
F
F
Rockefeller University, New York, NY 10021.
Galbraith
R A
RA
Sassa
S
S
D001798:Blood Proteins / Q000096:biosynthesis*; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D002455:Cell Division / Q000235:genetics; D006801:Humans; D011163:Hydroxymethylbilane Synthase / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D009174:Mycoplasma / Q000302:isolation & purification; D010641:Phenotype; D000623:Porphobilinogen Synthase / Q000378:metabolism; D011166:Porphyrins / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D001798:Blood Proteins; D011166:Porphyrins; D011163:Hydroxymethylbilane Synthase; D000623:Porphobilinogen Synthase