Protection of guinea-pigs against foot-and-mouth disease virus by immunization with a PhoE-FMDV hybrid protein.
A hybrid protein was constructed containing two antigenic determinants of the structural protein VP1 of foot-and-mouth disease virus, inserted in a cell surface-exposed region of Escherichia coli outer membrane protein PhoE. Immunization of guinea-pigs with partially purified protein resulted in high levels of neutralizing antibodies and complete protection against challenge with the virus.
Agterberg
M
M
Institute of Molecular Biology and Medical Biotechnology, University of Utrecht, The Netherlands.
Adriaanse
H
H
Barteling
S
S
van Maanen
K
K
Tommassen
J
J
D000595:Amino Acid Sequence; D000818:Animals; D005537:Aphthovirus / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D005536:Foot-and-Mouth Disease / Q000517:prevention & control*; D006168:Guinea Pigs; D007114:Immunization; D008969:Molecular Sequence Data; D018272:Porins
D001425:Bacterial Outer Membrane Proteins; D036022:Capsid Proteins; D018272:Porins; C442501:VP1 protein, Foot-and-mouth disease virus