Magnesium perchlorate and cis-platinum as radiosensitizers in in vitro radiolysis of nucleotides.
The mechanism of the gamma-radiolysis of the nucleotide guanosine-5'-monophosphate (5'-GMP) in the presence of Mg++ ions and cis-platinum was investigated. From high performance liquid chromatography and spectroscopy studies it was found that the OH radicals, produced in irradiated aqueous solutions, reacted with 5'-GMP by addition to the double bonds and that Mg++ as well as cis-Pt sensitized the nucleotide to radiation damage. Furthermore, an important finding was the fact that when the irradiation took place in aerated solutions, the Mg++ ions, contrary to cis-Pt, did not seem to have any effect on the decomposition yield G(-5'-GMP). This result together with the sensitization (100% increase) observed in deaerated solutions led to the conclusion that irradiation in the presence of Mg++ may result in a more effective gamma-radiation therapy for tumours.
Anastassopoulou
J D
JD
National Technical University of Athens, Laboratory of Radiation Chemistry and Biospectroscopy, Greece.
D002851:Chromatography, High Pressure Liquid; D002945:Cisplatin / Q000494:pharmacology*; D005720:Gamma Rays; D006150:Guanine Nucleotides / Q000528:radiation effects*; D006157:Guanosine Monophosphate / Q000528:radiation effects*; D006878:Hydroxides; D017665:Hydroxyl Radical; D008274:Magnesium / Q000494:pharmacology*; D017616:Magnesium Compounds; D015394:Molecular Structure; D010472:Perchlorates / Q000494:pharmacology*; D012996:Solutions; D013053:Spectrophotometry; D014867:Water
D006150:Guanine Nucleotides; D006878:Hydroxides; D017616:Magnesium Compounds; D010472:Perchlorates; D012996:Solutions; D014867:Water; D017665:Hydroxyl Radical; C016547:magnesium perchlorate; D006157:Guanosine Monophosphate; D008274:Magnesium; D002945:Cisplatin