Identification of a 32K plasma membrane protein that binds to the myristylated amino-terminal sequence of p60v-src.
The transforming protein of Rous sarcoma virus, p60v-src, is a myristylated membrane-bound phosphoprotein. Interaction of p60v-src with the plasma membrane is essential for transforming activity, and is mediated by association with a membrane-bound Src receptor protein. Evidence for the existence of an Src receptor is based on the ability of a myristylated peptide containing the N-terminal Src sequence to inhibit binding of p60v-src to plasma membranes in vitro: binding of p60v-src to a plasma membrane receptor is therefore mediated by N-terminal Src sequences. Here we report that a myristyl-Src peptide, but not the corresponding non-myristylated peptide, can be specifically crosslinked to a plasma membrane protein of relative molecular mass 32,000 (Mr32K). The 32K protein represents an Src-binding protein in the plasma membrane that is likely to be a component of the myristyl-Src receptor, and which could be involved in cellular transformation.
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Department of Biology, Lewis Thomas Laboratory, Princeton University, New Jersey 08544.
Ling
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D000595:Amino Acid Sequence; D000818:Animals; D003411:Arvicolinae; D001358:Avian Sarcoma Viruses; D002462:Cell Membrane / Q000378:metabolism; D003432:Cross-Linking Reagents; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009226:Myristates / Q000378:metabolism*; D009227:Myristic Acids / Q000378:metabolism*; D009842:Oligopeptides; D015688:Oncogene Protein pp60(v-src) / Q000378:metabolism*; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism*
D003432:Cross-Linking Reagents; D008565:Membrane Proteins; D009226:Myristates; D009227:Myristic Acids; D009842:Oligopeptides; D011956:Receptors, Cell Surface; D015688:Oncogene Protein pp60(v-src)