Translation initiation factor-dependent extracts from Saccharomyces cerevisiae.
Translation initiation factor 4A- and 4E-dependent extracts were developed from Saccharomyces cerevisiae and used to study factor requirements for translation of individual mRNAs in vitro. Whereas all mRNAs tested required eIF-4A, mRNAs devoid of secondary structure in their 5' untranslated region did not require exogenous eIF-4E for translation. The latter included alfalfa mosaic virus RNA4, mRNA containing the untranslated region of tobacco mosaic virus RNA and mRNA containing part of the untranslated region of poliovirus RNA. Furthermore, initiation of translation on mRNAs containing part of the untranslated region of poliovirus RNA is most likely internal.
Altmann
M
M
Institut für Biochemie und Molekularbiologie, Universität Bern, Switzerland.
Blum
S
S
Pelletier
J
J
Sonenberg
N
N
Wilson
T M
TM
Trachsel
H
H
D000818:Animals; D039601:Eukaryotic Initiation Factor-4A; D039561:Eukaryotic Initiation Factor-4E; D005796:Genes; D007700:Kinetics; D010442:Peptide Chain Initiation, Translational; D010448:Peptide Initiation Factors / Q000378:metabolism*; D010957:Plasmids; D017955:Poliovirus / Q000235:genetics; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D014027:Tobacco Mosaic Virus / Q000235:genetics; D014158:Transcription, Genetic
D039561:Eukaryotic Initiation Factor-4E; D010448:Peptide Initiation Factors; D012333:RNA, Messenger; D012367:RNA, Viral; D039601:Eukaryotic Initiation Factor-4A