Cloning and characterization of the proximal promoter region of the rat insulin-like growth factor I (IGF-I) receptor gene.
We have isolated genomic clones that contain the promoter region of the rat IGF-I receptor gene. A unique transcriptional start site was suggested by the results of primer extension and RNase protection assays, which also defined a 940-base 5'-untranslated region. Despite the single start site, the proximal 415 base pairs of 5'-flanking region were devoid of TATA or CCAAT elements. The region surrounding the start site was, however, similar to a recently described "initiator" sequence that can direct specific transcription initiation in the absence of a TATA element. The 5'-flanking region was GC-rich and contained several possible SP1 sites, but also included potential ETF and AP-2 binding sites. The rat IGF-I receptor gene promoter region appears to have some sequences similar to both "housekeeping" and highly regulated promoters and may be an example of an intermediary class of regulatory region.
Werner
H
H
Diabetes Branch, National Institutes of Health, Bethesda, Maryland 20892.
Stannard
B
B
Bach
M A
MA
LeRoith
D
D
Roberts
C T
CT
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D007334:Insulin-Like Growth Factor I; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011956:Receptors, Cell Surface / Q000235:genetics*; D017451:Receptors, Somatomedin; D012045:Regulatory Sequences, Nucleic Acid; D013002:Somatomedins; D014157:Transcription Factors / Q000378:metabolism
D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017451:Receptors, Somatomedin; D013002:Somatomedins; D014157:Transcription Factors; D007334:Insulin-Like Growth Factor I