title: Virucidal effect of ozone treatment of laboratory animal viruses.
abstract: An ozonization method was used to inactivate the viral pathogens of laboratory animals. Ozone at a concentration of over 100 ppm with high humidity was highly virucidal against 4 RNA viruses: HVJ, Theiler's murine encephalomyelitis virus (TMEV), Reo type 3 virus (RV) and murine hepatitis virus (MHV). For the ozone tests, 0.1 ml of a virus suspension in deionized water or saline and was placed in 35-mm dishes. The titer of 10(6) plaque-forming units of TMEV in a liquid-phase, which was highly stable against physical treatments, was reduced within 1 hr to a level of 0 by 300 ppm of ozone at 80% humidity and 22-25 degrees C. HVJ and MHV were more susceptible than TMEV to the ozone treatment. RV was the most resistant of the 4 viruses. The ozonization method may be a good way to disinfect not only for the laboratory animal RNA-viruses (both of enveloped and unenveloped viruses) but also animal rooms, clean rooms and even safety cabinets.
authors.affiliation: Laboratory Animal Center for Biomedical Research, Nagasaki University School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Sato
authors.forename: Y
authors.initials: Y
authors.lastname: Wananabe
authors.forename: H
authors.initials: H
authors.lastname: Miyata
mesh_terms: D000818:Animals; D000830:Animals, Laboratory / Q000382:microbiology*; D000998:Antiviral Agents; D003470:Culture Media; D004202:Disinfectants; D006813:Humidity; D012089:Mammalian orthoreovirus 3 / Q000187:drug effects; D004680:Maus Elberfeld virus / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D006517:Murine hepatitis virus / Q000187:drug effects; D010126:Ozone / Q000494:pharmacology*; D010222:Parainfluenza Virus 1, Human / Q000187:drug effects; D010710:Phosphates / Q000494:pharmacology; D012328:RNA Viruses / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D003470:Culture Media; D004202:Disinfectants; D010710:Phosphates; D010126:Ozone