Evaluation of a nonequilibrium isoelectric focusing (IEF) method for the simultaneous typing of esterase D (EsD), red cell acid phosphatase (AcP1), phosphoglucomutase (PGM1), adenylate kinase (AK), and adenosine deaminase (ADA).
A nonequilibrium isoelectric focusing method incorporating the chemical spacers MOPS and HEPES was developed and subsequently evaluated for its ability to reliably discriminate common and rare phenotypes in the esterase D (EsD), red cell acid phosphatase (AcP1), phosphoglucomutase (PGM1), adenylate kinase (AK), and adenosine deaminase (ADA) isoenzyme systems. The validation procedures used were blind testing, comparison of results to conventional methods, and evaluation of known rare variant phenotypes. This method proved to be a quick and reliable method for typing all five isoenzyme systems, while providing an excellent probability of discrimination (PD = 0.96).
Stockwell
D C
DC
San Bernardino County Sheriff's Regional Forensic Science Laboratory, CA.
Gregonis
D J
DJ
Jones
D T
DT
D000135:Acid Phosphatase / Q000097:blood* / Q000235:genetics; D000243:Adenosine Deaminase / Q000097:blood* / Q000235:genetics; D000263:Adenylate Kinase / Q000097:blood* / Q000235:genetics; D000483:Alleles; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000097:blood* / Q000235:genetics; D016009:Chi-Square Distribution; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000097:blood / Q000235:genetics; D009700:Nucleoside Deaminases / Q000097:blood*; D010641:Phenotype; D010733:Phosphoglucomutase / Q000097:blood* / Q000235:genetics; D010770:Phosphotransferases / Q000097:blood*; D011237:Predictive Value of Tests
D007527:Isoenzymes; D010770:Phosphotransferases; D000263:Adenylate Kinase; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C020118:ESD protein, human; D000135:Acid Phosphatase; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase; D010733:Phosphoglucomutase