Serotypes and electropherotypes of human rotavirus in the USA: 1987-1989.
The epidemiology of rotavirus gastroenteritis was investigated for two consecutive seasons (1987-1988 and 1988-1989) in seven locales in the continental USA. The 281 representative fecal samples obtained from children with diarrhea were electropherotyped and serotyped by an enzyme immunoassay with serotype-specific monoclonal antibodies and a new amplification typing technique (polymerase chain reaction typing). Serotype 1 was predominant in both years, particularly in the North and East; serotype 3 was second in frequency and found most often in the South; serotype 2 was detected only occasionally; serotypes 4, 8, and 9 were never found. Rotavirus strains were grouped into five major electropherotypes, each corresponded to a single serotype, and the relative migration of the gene segments 7-9 could be used to distinguish serotype 1 from serotype 3. The amplification typing technique proved to be of great value in typing the 17% of rotavirus-positive specimens untypable by the serologic technique.
Gouvea
V
V
Viral Gastroenteritis Unit, CDC, Atlanta, GA 30333.
Ho
M S
MS
Glass
R
R
Woods
P
P
Forrester
B
B
Robinson
C
C
Ashley
R
R
Riepenhoff-Talty
M
M
Clark
H F
HF
Taniguchi
K
K
D000911:Antibodies, Monoclonal; D002648:Child; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000382:microbiology; D005759:Gastroenteritis / Q000453:epidemiology* / Q000382:microbiology; D006801:Humans; D007124:Immunoenzyme Techniques; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D012401:Rotavirus / Q000145:classification* / Q000235:genetics; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000382:microbiology; D012621:Seasons; D012703:Serotyping; D014481:United States / Q000453:epidemiology
D000911:Antibodies, Monoclonal; D012367:RNA, Viral