15N NMR spectroscopy of Pseudomonas cytochrome c-551.
15N-1H correlation spectroscopy with detection at the 1H frequency has been used at natural abundance to detect nitrogen nuclei bonded to protons in the ferrocytochrome c-551 from Pseudomonas aeruginosa (ATCC 19429). Side-chain aromatic nitrogens, main-chain amides, and side-chain amides have been assigned to specific residues by comparison to previous proton assignments. Assignment ambiguities arising from overlap in the proton dimension have been resolved by examining spectra as a function of temperature and pH. Nitrogen chemical shifts are reported at pH 4.6 and 9.4 and three temperatures, 32, 50, and 60 degrees C. Significant differences arise from the observed protein shifts and expected shifts in the random coil polypeptide.
Timkovich
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Department of Chemistry, University of Alabama, Tuscaloosa 35487-0336.
D001426:Bacterial Proteins; D003574:Cytochrome c Group / Q000737:chemistry*; D006859:Hydrogen; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D009587:Nitrogen Isotopes; D011487:Protein Conformation; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D013696:Temperature
D001426:Bacterial Proteins; D003574:Cytochrome c Group; D009587:Nitrogen Isotopes; D006859:Hydrogen; C017167:cytochrome C(551)