title: Application of countercurrent chromatography/thermospray mass spectrometry for the identification of bioactive lignans from plant natural products.
abstract: The versatility and resolving power of countercurrent chromatography (CCC) has been demonstrated with a newly developed analytical high-speed planet centrifuge system. Interfacing countercurrent chromatography with mass spectrometry (MS) provides a new analytical methodology that integrates the advantages of countercurrent chromatography with the low detection limit and identification capability of mass spectrometry. In this paper the capability of thermospray CCC/MS is demonstrated in identifying and validating the bioactive and structurally known lignans from a crude extract of Schisandra rubriflora Rhed et Wils, a traditional Chinese herbal medicine for treatment of hepatitis.
authors.affiliation: Research Triangle Institute, North Carolina 27709.
authors.forename: Y W
authors.initials: YW
authors.lastname: Lee
authors.forename: R D
authors.initials: RD
authors.lastname: Voyksner
authors.forename: T W
authors.initials: TW
authors.lastname: Pack
authors.forename: C E
authors.initials: CE
authors.lastname: Cook
authors.forename: Q C
authors.initials: QC
authors.lastname: Fang
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
mesh_terms: D002845:Chromatography / Q000379:methods*; D017705:Lignans; D008031:Lignin / Q000032:analysis*; D013058:Mass Spectrometry; D010944:Plants / Q000032:analysis*
chemical_list: D017705:Lignans; D008031:Lignin