Expression of HSV-1 glycoproteins in tunicamycin-treated monkey kidney cells.
The role of glycosylation in transport and expression of HSV-1 glycoproteins on the surface of HSV-1-infected African green monkey kidney cells was investigated by using tunicamycin (TM). A concentration of 0.05 microgram/ml of TM inhibited the replication of HSV-1 by greater than 99%. Immunoblot analysis of TM-treated and virus-infected cells indicated that 0.05 microgram/ml of TM blocked the addition of N-linked oligosaccharides into glycoproteins B, C and D. An immunofluorescence assay of TM-treated (0.05 and 0.1 microgram/ml) and virus-infected cells demonstrated the presence of nonglycosylated gC, gD and a reduced amount of gB on the surface of infected cells. The results suggest that the addition of N-linked oligosaccharides on the studied HSV-1 glycoproteins was not necessary for their transport and expression on the virus-infected cell surface.
Chatterjee
S
S
Department of Pediatrics, University of Alabama, Birmingham 35294.
Nishimuro
S
S
Whitley
R J
RJ
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D007668:Kidney; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000302:isolation & purification; D018139:Simplexvirus / Q000187:drug effects / Q000502:physiology*; D014415:Tunicamycin / Q000494:pharmacology*; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000187:drug effects
D008562:Membrane Glycoproteins; D014415:Tunicamycin