A simple and rapid procedure for the direct detection of cytomegalovirus in urine samples.
We compared cytomegalovirus (CMV) isolation in tissue culture and viral DNA detection by DNA:DNA hybridization in 60 clinical urine samples concentrated by different procedures. Our results showed that urine concentration by filtration is the easiest and quickest procedure allowing further detection of CMV. With optimized working conditions, the CMV detection in urine can be done in 5 hr without requiring cell cultures or costly instruments.
Landini
M P
MP
Institute of Microbiology, Faculty of Medicine, University of Bolonga, Italy.
Trevisani
B
B
Guan
M X
MX
Ripalti
A
A
Lazzarotto
T
T
La Placa
M
M
D046508:Culture Techniques; D003587:Cytomegalovirus / Q000302:isolation & purification*; D015342:DNA Probes; D004279:DNA, Viral; D005374:Filtration; D006801:Humans; D015151:Immunoblotting; D009693:Nucleic Acid Hybridization; D014461:Ultracentrifugation; D014556:Urine / Q000382:microbiology*
D015342:DNA Probes; D004279:DNA, Viral