title: Induction of DNA damage by dimethylarsine, a metabolite of inorganic arsenics, is for the major part likely due to its peroxyl radical.
abstract: To reveal the mechanisms of previously reported lung-specific DNA strand scissions in murine after oral administration of dimethylarsinic acid (DMAA), a main metabolite of inorganic arsenics in mammals, the ultimate substance causing DNA lesion was investigated using dimethylarsine which was a further metabolite of DMAA. The alkaline elution assay using 3H-labeled DNA showed that a major portion of the strand breaks was not suppressed by SOD and catalase, suggesting an ultimate substance other than active oxygen participated in the DNA damage. By ESR analysis, a radical estimated to be (CH3)2AsOO. was detected as a reaction product of dimethylarsine and molecular oxygen. This peroxyl radical, rather than active oxygen, was assumed to play a major role in DNA damage.
authors.affiliation: Department of Hygienic Chemistry, Nihon University College of Pharmacy, Chiba, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yamanaka
authors.forename: M
authors.initials: M
authors.lastname: Hoshino
authors.forename: M
authors.initials: M
authors.lastname: Okamoto
authors.forename: R
authors.initials: R
authors.lastname: Sawamura
authors.forename: A
authors.initials: A
authors.lastname: Hasegawa
authors.forename: S
authors.initials: S
authors.lastname: Okada
mesh_terms: D020261:Arsenic Poisoning; D001152:Arsenicals; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D006801:Humans; D066298:In Vitro Techniques; D007739:L Cells; D010100:Oxygen; D013481:Superoxides
chemical_list: D001152:Arsenicals; D005609:Free Radicals; D013481:Superoxides; C062856:dimethylarsine; D004247:DNA; D010100:Oxygen