title: A one-step enzyme immunoassay for human manganese superoxide dismutase with monoclonal antibodies.
abstract: A one-step enzyme immunoassay for the determination of manganese superoxide dismutase in serum has been developed with two kinds of monoclonal antibodies. Proposed method had high sensitivity (assay range, 0.4-200 ng/ml), good recovery (recovery percentage, 102.9-106.2%) and reproducibility (intraassay, C.V. = 1.87-3.66%; interassay, C.V. = 3.03-10.4%). From these results, it is possible to apply this method to routine clinical analysis and biochemical research with various purposes.
authors.affiliation: Department of Pharmaceutics, Gifu Pharmaceutical University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Adachi
authors.forename: K
authors.initials: K
authors.lastname: Hirano
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Muto
authors.forename: F
authors.initials: F
authors.lastname: Okuno
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D006801:Humans; D006825:Hybridomas; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D013482:Superoxide Dismutase / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D013482:Superoxide Dismutase