[The cytological and immunological characteristics of a virus-producing FLK 44/2 culture].
Results obtained from cytological and immunological analysis of FLK 44/2 cells indicate the transformation and heterogenization of BLV-producing cell culture. Karyotypic changes of the culture were observed, as well as alterations in its antigenic specificity leading to antigenic diversion. The cell population displayed a high proliferation activity limited by topoinhibition and culture medium exhaustion. An immunocytochemical investigation of virus-producing cell population, using monoclonal and polyclonal antibodies against BLV revealed various cellular forms of BLV-expression. A possibility is discussed to use the immunological approach for selection and establishment of cellular clones exhibiting appropriate virus production parameters.
Adomaĭtene
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DI
Nemeĭkaĭte
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Sadauskas
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D000818:Animals; D000911:Antibodies, Monoclonal; D002455:Cell Division; D002461:Cell Line, Transformed; D002478:Cells, Cultured / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D004622:Embryo, Mammalian; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D005779:Immunodiffusion; D007150:Immunohistochemistry; D007621:Karyotyping; D007668:Kidney / Q000166:cytology* / Q000276:immunology / Q000382:microbiology; D001909:Leukemia Virus, Bovine / Q000235:genetics / Q000254:growth & development*; D012756:Sheep; D014776:Virus Cultivation / Q000379:methods
D000911:Antibodies, Monoclonal
Tsitologicheskaia i immunologicheskaia kharakteristika virusprodutsiruiushcheĭ kul'tury FLK 44/2.