Superoxide generation by neutrophils after exposure to a conventional peritoneal dialysis solution for different time periods.
Neutrophils were isolated from canine blood and exposed to a conventional, acidic, lactate-containing peritoneal dialysis solution for 0, 2 and 4 minutes in one study and 0, 4, 7 and 10 minutes in another. Superoxide generation, expressed in nanomoles per million cells, was determined using a method based on the superoxide dismutase-inhibitable reduction of ferricytochrome c. Brief exposure of neutrophils to a conventional dialysis solution could significantly inhibit the generation of superoxide by neutrophils.
Gupta
D K
DK
Veterans Administration Hospital, Hines, Illinois.
Ing
B L
BL
Manahan
F J
FJ
Zhou
F Q
FQ
Yu
A W
AW
Nawab
Z M
ZM
Rahman
M A
MA
D000818:Animals; D015314:Dialysis Solutions / Q000494:pharmacology*; D004285:Dogs; D007773:Lactates / Q000378:metabolism; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010530:Peritoneal Dialysis; D013481:Superoxides / Q000378:metabolism*; D013997:Time Factors
D015314:Dialysis Solutions; D007773:Lactates; D013481:Superoxides