title: Herpes viral hepatitis in a toucan.
abstract: Herpesvirus infection was diagnosed in a toucan. The herpesvirus was isolated from the liver and identified by electron microscopy in the liver and in cell culture. A negative immunofluorescent reaction was obtained when virus-infected cell cultures were reacted with a conjugate to the herpesvirus of Pacheco's disease. The main pathologic finding in the toucan consisted of a severe necrotizing hepatitis with intranuclear inclusions in the liver and spleen. A presumptive diagnosis of chlamydiosis was also made, based on a positive direct fluorescent monoclonal antibody reaction to chlamydial antigens in impression smears of liver and spleen. Chlamydial isolation attempts were unsuccessful. The toucan had been in contact with two macaws that had died 5 days before the toucan died and were diagnosed by histology as having herpesvirus hepatitis.
authors.affiliation: California Veterinary Diagnostic Laboratory System, School of Veterinary Medicine, University of California-Davis, Turlock 95381.
authors.forename: B R
authors.initials: BR
authors.lastname: Charlton
authors.forename: B C
authors.initials: BC
authors.lastname: Barr
authors.forename: A E
authors.initials: AE
authors.lastname: Castro
authors.forename: P L
authors.initials: PL
authors.lastname: Davis
authors.forename: B J
authors.initials: BJ
authors.lastname: Reynolds
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000382:microbiology* / Q000473:pathology; D001717:Birds; D005260:Female; D006524:Hepatitis, Viral, Animal / Q000382:microbiology* / Q000473:pathology; D006564:Herpesviridae / Q000302:isolation & purification* / Q000648:ultrastructure; D006566:Herpesviridae Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D008099:Liver / Q000473:pathology; D008854:Microscopy, Electron; D013154:Spleen / Q000473:pathology