Plasma concentrations of endothelin-1 in spontaneously hypertensive rats and DOCA-salt hypertensive rats.
To investigate the possible involvement of endothelin-1 (ET-1), an endothelium-derived potent vasoconstrictor peptide, in the pathophysiology of hypertension, plasma ET-1 levels in 15-week-old spontaneously hypertensive rats (SHR) and DOCA-salt hypertensive rats were measured with a sandwich-type enzyme immunoassay. The vasocontractile effect of ET-1 in aortic helical preparations was significantly more sensitive in DOCA-salt hypertensive rats than in control sham-operated rats, but plasma levels of ET-1 did not differ between them. Plasma ET-1 levels in genetically hypertensive rats (SHR and stroke-prone SHR) were significantly lower than those in age-matched normotensive Wistar-Kyoto (WKY) rats. The plasma concentrations of big ET-1, a precursor of ET-1, in both SHR and SHR-SP were significantly lower than those of WKY, suggesting that the production of ET-1 is decreased in rats of genetic hypertension. Although the vascular reactivity to ET-1 increased in both DOCA-salt hypertensive and genetically hypertensive rats, present findings of the plasma ET-1 levels suggest that the role of ET-1 in the vascular control system may be different in DOCA-salt hypertensive rats and genetically hypertensive rats.
Suzuki
N
N
Tsukuba Research Laboratories, Takeda Chemical Industries, Ltd., Ibaraki, Japan.
Miyauchi
T
T
Tomobe
Y
Y
Matsumoto
H
H
Goto
K
K
Masaki
T
T
Fujino
M
M
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000503:physiopathology; D002851:Chromatography, High Pressure Liquid; D003900:Desoxycorticosterone; D016232:Endothelins; D004730:Endothelium, Vascular; D006973:Hypertension / Q000097:blood* / Q000139:chemically induced; D066298:In Vitro Techniques; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000503:physiopathology; D010455:Peptides / Q000097:blood* / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D011918:Rats, Inbred SHR / Q000097:blood*; D011919:Rats, Inbred Strains / Q000097:blood*; D012016:Reference Values; D014664:Vasodilation / Q000187:drug effects
D016232:Endothelins; D010455:Peptides; D003900:Desoxycorticosterone; D000109:Acetylcholine