Early death after feline infectious peritonitis virus challenge due to recombinant vaccinia virus immunization.
The gene encoding the fusogenic spike protein of the coronavirus causing feline infectious peritonitis was recombined into the genome of vaccinia virus. The recombinant induced spike-protein-specific, in vitro neutralizing antibodies in mice. When kittens were immunized with the recombinant, low titers of neutralizing antibodies were obtained. After challenge with feline infectious peritonitis virus, these animals succumbed earlier than did the control group immunized with wild-type vaccinia virus (early death syndrome).
Vennema
H
H
Department of Virology, Faculty of Veterinary Medicine, State University of Utrecht, The Netherlands.
de Groot
R J
RJ
Harbour
D A
DA
Dalderup
M
M
Gruffydd-Jones
T
T
Horzinek
M C
MC
Spaan
W J
WJ
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000917:Antibody Formation; D002371:Cat Diseases / Q000276:immunology / Q000382:microbiology* / Q000401:mortality; D002415:Cats; D003332:Coronaviridae / Q000235:genetics / Q000276:immunology; D003333:Coronaviridae Infections / Q000276:immunology / Q000401:mortality / Q000662:veterinary*; D005814:Genes, Viral; D007116:Immunization, Passive; D051379:Mice; D009500:Neutralization Tests; D010538:Peritonitis / Q000276:immunology / Q000401:mortality / Q000662:veterinary*; D011994:Recombinant Proteins / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology
D000914:Antibodies, Viral; D011994:Recombinant Proteins; D014764:Viral Proteins