title: Mapping of low abundance latency-associated RNA in the trigeminal ganglia of mice latently infected with herpes simplex virus type 1.
abstract: During herpes simplex virus type 1 (HSV-1) latent infection of the mouse trigeminal ganglion there is limited viral gene expression. The latency-associated transcripts (LAT) map approximately to the PstI-MluI fragment within the BamHI B and BamHI E fragments (long repeat regions) of the viral genome. Additional weak hybridization signals have been detected by in situ hybridization that correspond to transcription from HSV-1 DNA fragments adjacent tot he PstI-MluI fragment. We mapped the region encoding this additional transcription. This minor latency-associated RNA (m-LAT) was shown to map to a group of contiguous fragments (approximately 8.3 kb of DNA), which are adjacent to the 3' end of LAT and to a (2.0 kb) fragment adjacent to the 5' end of the LAT. Using single-stranded probes in in situ hybridization experiments, we showed that the KpnI-BamHI and BamHI-SacI regions of m-LAT are transcribed in a rightward direction within the long internal repeat region. This low abundance RNA may be related to the previously described LAT.
authors.affiliation: Wistar Institute, Philadelphia, Pennsylvania 19104.
authors.forename: W J
authors.initials: WJ
authors.lastname: Mitchell
authors.forename: R P
authors.initials: RP
authors.lastname: Lirette
authors.forename: N W
authors.initials: NW
authors.lastname: Fraser
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015342:DNA Probes; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis*; D015183:Restriction Mapping; D018139:Simplexvirus / Q000235:genetics*; D014158:Transcription, Genetic; D012668:Trigeminal Ganglion / Q000382:microbiology*
chemical_list: D015342:DNA Probes; D012367:RNA, Viral