Molecular and functional analysis of the ruv region of Escherichia coli K-12 reveals three genes involved in DNA repair and recombination.
Recombinant plasmids carrying ruvA, ruvB, or both were constructed and used to investigate the genetic defects in a collection of UV-sensitive ruv mutants. The results revealed that efficient survival of UV-irradiated cells depends on both ruvA and ruvB, and on a third gene, ruvC, located upstream of the ruvAB operon. Southern blotting analysis was used to locate insertions in ruv and to examine putative deletion mutants. Two Tn10 insertions were located to the region encoding ruvA. Since these insertions caused a deficiency in the activities of both ruvA and ruvB, we concluded that they must exert a polar effect on ruvB. Two putative ruv deletion mutants were shown to be the result of deletion-inversion events mediated during imprecise excision of Tn10. The relevant inversion breakpoints in these mutants were located to ruvA and ruvC.
Sharples
G J
GJ
Genetics Department, University of Nottingham, Queens Medical Centre, UK.
Benson
F E
FE
Illing
G T
GT
Lloyd
R G
RG
D015139:Blotting, Southern; D004260:DNA Repair; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics* / Q000528:radiation effects; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D009154:Mutation; D010957:Plasmids; D011995:Recombination, Genetic / Q000235:genetics; D015183:Restriction Mapping; D014466:Ultraviolet Rays
D004251:DNA Transposable Elements; D004269:DNA, Bacterial