Cross-linking of endothelin 1 and endothelin 3 to rat brain membranes: identification of the putative receptor(s).
Affinity-labeling experiments with 125I-endothelin derivatives using bifunctional cross-linking reagents were carried out in an attempt to identify the polypeptide component(s) of the endothelin/sarafotoxin receptors in rat brain tissues. In rat cerebellum, cortex, and caudate putamen, endothelin 1 specifically labeled a major component with a molecular mass of around 53,000. In the same tissues endothelin 3 specifically labeled, in addition to the 53,000 band, a band of molecular mass of 38,000. This result clearly indicates that in the brain the endothelin binding site resides within a polypeptide of apparent Mr = 53,000. The possible presence of receptor subtypes is discussed with reference also to the reported identification of endothelin receptors in chick cardiac membrane and in rat mesangial cells.
Ambar
I
I
Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.
Kloog
Y
Y
Sokolovsky
M
M
D000345:Affinity Labels; D000818:Animals; D001665:Binding Sites; D001923:Brain Chemistry; D002462:Cell Membrane / Q000378:metabolism; D003432:Cross-Linking Reagents; D016232:Endothelins / Q000378:metabolism*; D008297:Male; D051381:Rats; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000378:metabolism*; D011950:Receptors, Cholinergic / Q000302:isolation & purification / Q000378:metabolism; D017466:Receptors, Endothelin; D018000:Receptors, Peptide; D014443:Tyrosine
D000345:Affinity Labels; D003432:Cross-Linking Reagents; D016232:Endothelins; D011956:Receptors, Cell Surface; D011950:Receptors, Cholinergic; D017466:Receptors, Endothelin; D018000:Receptors, Peptide; C058085:sarafotoxin receptor; D014443:Tyrosine