[Lung water changes after the intratracheal infusion of bile acid in dogs--a new method for its measurement by the double indicator dilution method using heat and sodium ions].
We evaluated a double indicator dilution method of lung water for studying the effect of intratracheally infused bile acids on the respiratory system in anesthetized dogs. Deoxycholic acid and taurodeoxycholic acid were diluted to 1% or 10% with normal saline solution, and 1 ml.kg-1 was infused into the trachea through an endotracheal tube under controlled ventilation with air. The lung water volumes determined by the thermo-saline method correlated with those obtained by gravimetry at sacrifice. In real-time recording obtained by the thermo-saline method, the lung water volumes increased following injection of bile acid, and the increase varied with the bile acids and its concentration. With 10% diluted deoxycholic acid, lung water volume increased linearly to 28.9 +/- 3.5 ml.kg-1 after 1 hour. With 10% diluted taurodeoxycholic acid and 1% diluted deoxycholic acid, lung water volume increased rapidly until 3 hours and then slowly to 22.3 +/- 0.9 ml.kg-1 and 21.4 +/- 3.5 ml.kg-1 after 6 hours. With 1% diluted taurodeoxycholic acid, the lung water volume increased slightly to 15.7 +/- 2.9 after 6 hours. The thermo-saline method is considered to be useful for clinical evaluation of pulmonary edema.
Kaneko
T
T
First Department of Surgery, Kumamoto University Medical School.
D000818:Animals; D001647:Bile Acids and Salts / Q000008:administration & dosage*; D004285:Dogs; D015633:Extravascular Lung Water / Q000378:metabolism*; D007201:Indicator Dilution Techniques; D007442:Intubation, Intratracheal; D008168:Lung / Q000502:physiology*
D001647:Bile Acids and Salts