Postnatal development of voltage-dependent calcium channels in the mouse brain disclosed by the Xenopus oocyte assay.
We assessed the ontogeny of murine voltage-dependent calcium channels by extracting mRNA from brains of mice at different postnatal ages and injecting the mRNA into oocytes of the frog, Xenopus laevis. Voltage-dependent Ca2(+)-activated Cl- channels were measured to assess the presence of Ca2(+) channels. When compared with water-injected oocytes (controls), an increase in Ca2(+) channels was not detected until postnatal day 7. The number of Ca2+ channels peaked between 9 and 18 days and began to decline by 35 days. Bath application of barium, serotonin and the Ca2+ channel antagonist, verapamil, to mRNA-injected oocytes confirmed the presence of Ca2+ channels.
Cohen
R W
RW
Mental Retardation Research Center, University of California, Los Angeles.
Campagnoni
A T
AT
Hull
C D
CD
D000375:Aging / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D001921:Brain / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D008564:Membrane Potentials; D051379:Mice; D009865:Oocytes / Q000378:metabolism* / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism* / Q000502:physiology; D012701:Serotonin / Q000494:pharmacology; D014982:Xenopus laevis
D015220:Calcium Channels; D012333:RNA, Messenger; D012701:Serotonin