Genetic mapping of a cloned sequence responsible for susceptibility to ecotropic murine leukemia viruses.
A mouse cDNA that confers susceptibility to ecotropic murine leukemia viruses following transfection into human EJ cells has been cloned and sequenced. We show that this sequence is likely to be Rec-1, the chromosome 5 locus originally defined by studies with somatic cell hybrids as responsible for virus susceptibility, and provide a specific chromosomal map position for this locus by analysis of an interspecies backcross. This locus maps in the distal region of chromosome 5 and is thus not within the cluster of retrovirus-related genes near the centromere.
Kozak
C A
CA
National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
Albritton
L M
LM
Cunningham
J
J
D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D020022:Genetic Predisposition to Disease; D006801:Humans; D006822:Hybrid Cells; D009052:Leukemia Virus, Murine / Q000472:pathogenicity*; D007942:Leukemia, Experimental / Q000235:genetics* / Q000382:microbiology; D051379:Mice; D008970:Molecular Weight; D011991:Receptors, Virus / Q000235:genetics; D011995:Recombination, Genetic; D014162:Transfection
D011991:Receptors, Virus; D004247:DNA