A new selective, differential agar medium for isolation of Vibrio cholerae O1: PMT (polymyxin-mannose-tellurite) agar.
A new selective and differential agar medium, polymyxin-mannose-tellurite (PMT) agar was devised to differentiate easily colonies of Vibrio cholerae O1 from those of V. cholerae non-O1. The differentiation between colonies of the two vibrios is based on mannose-fermentation. Colonies of V. cholerae O1 on the agar are agglutinated with O1 antiserum of V. cholerae much more easily than those on thiosulfate-citrate-bile salts-sucrose (TCBS) agar.
Shimada
T
T
Department of Bacteriology, National Institute of Health, Tokyo.
Sakazaki
R
R
Fujimura
S
S
Niwano
K
K
Mishina
M
M
Takizawa
K
K
D000362:Agar; D003470:Culture Media; D008358:Mannose; D011113:Polymyxins; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013691:Tellurium; D014734:Vibrio cholerae / Q000302:isolation & purification*
D003470:Culture Media; D011113:Polymyxins; D000362:Agar; C026660:tellurous acid; D013691:Tellurium; D008358:Mannose