title: Increased density of peripheral benzodiazepine-binding sites in ovarian carcinomas as compared with benign ovarian tumours and normal ovaries.
abstract: 1. Benzodiazepines are involved in the control of proliferation and differentiation of normal and malignant cells in vitro. This regulatory ability is probably mediated via peripheral benzodiazepine-binding sites. 2. In the present study we compared the binding characteristics of peripheral benzodiazepine-binding sites in human epithelial ovarian carcinoma with those in benign ovarian tumours and normal ovaries. 3. The affinity and density of peripheral benzodiazepine-binding sites in homogenate preparations of ovarian carcinoma as compared with benign ovarian tumours and with normal tissues (used as controls) were determined using a ligand specific for peripheral benzodiazepine-binding sites, [3H]PK 11195, an isoquinoline carboxamide derivative. 4. We observed a robust (three- to five-fold) increase in the neoplasm compared with benign ovarian tumours and normal tissues, without a concomitant change in affinity values. 5. This finding may reflect a change in the metabolic rates of ovarian cancer which is expressed as the alteration in the density of peripheral benzodiazepine-binding sites.
authors.affiliation: Rappaport Family Institute for Research in the Medical Sciences, Haifa, Israel.
authors.forename: Y
authors.initials: Y
authors.lastname: Katz
authors.forename: G
authors.initials: G
authors.lastname: Ben-Baruch
authors.forename: Y
authors.initials: Y
authors.lastname: Kloog
authors.forename: J
authors.initials: J
authors.lastname: Menczer
authors.forename: M
authors.initials: M
authors.lastname: Gavish
mesh_terms: D000231:Adenocarcinoma, Papillary / Q000378:metabolism*; D000328:Adult; D000368:Aged; D001569:Benzodiazepines / Q000378:metabolism*; D001665:Binding Sites; D005260:Female; D006801:Humans; D007546:Isoquinolines / Q000378:metabolism; D008875:Middle Aged; D010049:Ovarian Diseases / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism*; D011963:Receptors, GABA-A / Q000378:metabolism*
chemical_list: D007546:Isoquinolines; D011963:Receptors, GABA-A; D001569:Benzodiazepines; C037850:PK 11195