Comparative studies of HSV-1 antigens solubilised from infected cells by using non-ionic or zwitterionic detergents.
HSV-1 antigen preparations solubilised from Vero cells by using either the non-ionic detergent Nonidet P40 or the zwitterionic detergent Empigen BB, and purified on sucrose density gradients or over a sucrose cushion, were tested by ELISA with anti-HSV-1 glycoprotein monoclonal antibodies and by radioimmunoprecipitation (RIP) with polyclonal HSV-1 antiserum. Amongst several proteins detected in these preparations, the four major HSV-1 glycoproteins, gB, gC, gD, and gE, were found to be present. Differences between NP40 or Empigen-solubilised HSV-1 antigen preparations with respect to two of these glycoproteins, gB and gE, were detected by using a small panel of monoclonal antibodies. Comparative studies in mice showed the Empigen-solubilised HSV-1 antigen preparations elicited greater antibody responses and greater protection against lethal HSV-1 challenge infection than the NP40-solubilised preparation.
Jennings
R
R
Department of Virology, Medical School, University of Sheffield, England.
Erturk
M
M
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000276:immunology / Q000302:isolation & purification*; D003902:Detergents / Q000145:classification / Q000494:pharmacology*; D006561:Herpes Simplex / Q000517:prevention & control; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D017830:Octoxynol; D009930:Organic Chemicals; D011092:Polyethylene Glycols / Q000494:pharmacology*; D018139:Simplexvirus / Q000032:analysis / Q000276:immunology*; D013501:Surface-Active Agents / Q000494:pharmacology*; D014709:Vero Cells / Q000032:analysis; D014759:Viral Envelope Proteins / Q000276:immunology / Q000302:isolation & purification; D014765:Viral Vaccines / Q000276:immunology / Q000302:isolation & purification*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D003902:Detergents; D009930:Organic Chemicals; D013501:Surface-Active Agents; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; C041772:empigen BB