Molecular characterization by RNA-RNA hybridization of a serotype 8 human rotavirus with "super-short" RNA electropherotype.
A human rotavirus isolate (the 69M strain) with a "super-short" RNA electropherotype has been classified as serotype 8, a new human serotype [Matsuno et al., 1985]RNA-RNA hybridization using 32P-labeled transcription probes was used to assess the genetic relatedness of the 69M strain to a panel of human and animal rotaviruses. The 69M strain showed a medium level of homology with subgroup 1, serotype 2 human rotaviruses with short RNA electropherotypes. However, the 69M strain was not significantly related to any other human rotavirus strains tested in this study, including recently identified serotype 9 strains (represented by the WI61 strain) and subgroup 1 human rotaviruses with long RNA electropherotypes (represented by the AU-1 strain) that are shown to be genetically distinct from other human rotavirus strains. Unexpectedly, a medium level of homology was also found between the 69M probe and the double-stranded RNAs from bovine rotavirus strains (represented by the NCDV strain).
Ohshima
A
A
Department of Third Internal Medicine, Akita University Hospital and Medical School, Japan.
Takagi
T
T
Nakagomi
T
T
Matsuno
S
S
Nakagomi
O
O
D000818:Animals; D014644:Genetic Variation / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis*; D012401:Rotavirus / Q000145:classification / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D012703:Serotyping
D012367:RNA, Viral