title: Tn5030: a conjugative transposon conferring clindamycin resistance in Bacteroides species.
abstract: Several examples of conjugal transfer of antibiotic resistance determinants in the absence of plasmid DNA have been described in gram-positive species. Previous work had suggested that transfer of clindamycin resistance among the gram-negative anaerobic Bacteroides species might occur by a similar mechanism. We report here the characterization of Tn5030, a 45-kb +/- 5-kb chromosomal, mobile genetic element devoid of the detectable terminal repeats typically associated with gram-negative transposons. Tn5030 transfers clindamycin resistance (ermFU) and sequences homologous to tetF in the absence of detectable plasmid DNA. This element represents a novel mechanism of genetic exchange among gram-negative organisms.
authors.affiliation: Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond.
authors.forename: M
authors.initials: M
authors.lastname: Halula
authors.forename: F L
authors.initials: FL
authors.lastname: Macrina
mesh_terms: D001439:Bacteroides / Q000187:drug effects* / Q000235:genetics; D001483:Base Sequence; D002981:Clindamycin / Q000494:pharmacology*; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D015723:Gene Library; D008969:Molecular Sequence Data; D015183:Restriction Mapping
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D002981:Clindamycin