title: Direct coupling of the somatostatin receptor to potassium channels by a G protein.
abstract: G proteins couple receptors to ionic channels indirectly by acting on membrane enzymes which modulate channel activity through second or third messengers such as cytoplasmic kinases, IP3 or Ca++. Recently, it has been shown that G proteins can act on ionic channels in a membrane-delimited or direct manner; from our experience this phenomenon seems to be widespread. A G protein purified from human red blood cells (hRBC) Gk when preactivated with GTP gamma S acts directly on muscarinic acetylcholine receptor-regulated K+ channels (K+[ACh]) in atrial cells and the stimulatory regulator of adenylyl cyclase, Gs from hRBCs acts directly on two distinct voltage-gated Ca++ channels, one in cardiac muscle and the other in skeletal muscle T-tubules. In many cells, including clonal GH3 pituitary cells, somatostatin (SST) inhibits secretion by a complex mechanism that involves a pertussis toxin (PTX)-sensitive step. This is not due to lowering cAMP since secretion induced by cAMP analogs and K+ depolarization are also inhibited. SST also causes membrane hyperpolarization, which is similar to the effect of ACh on cardiac pacemaking cells and may lead to decreases in intracellular Ca++ needed for secretion. ACh acting through a muscarinic recpetor in GH3 cells has the same effects as SST.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030.
authors.forename: A
authors.initials: A
authors.lastname: Yatani
authors.forename: L
authors.initials: L
authors.lastname: Birnbaumer
authors.forename: A M
authors.initials: AM
authors.lastname: Brown
mesh_terms: D000818:Animals; D002478:Cells, Cultured / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000502:physiology*; D006325:Heart Atria / Q000378:metabolism; D006801:Humans; D015221:Potassium Channels / Q000378:metabolism*; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D017481:Receptors, Somatostatin
chemical_list: D015221:Potassium Channels; D017981:Receptors, Neurotransmitter; D017481:Receptors, Somatostatin; D019204:GTP-Binding Proteins