Differential regulation of the N-myc gene in transfected cells and transgenic mice.
The N-myc gene is expressed specifically in the early developmental stages of numerous cell lineages. To assay for sequences that could potentially regulate N-myc expression, we transfected constructs that contained murine N-myc genomic sequences linked to a reporter gene and genomic clones that contained the complete human or murine N-myc genes into cell lines that either express or do not express the endogenous N-myc gene. Following either transient or stable transfection, the introduced N-myc sequences were expressed regardless of the expression status of the endogenous gene. In contrast, when the clones containing the complete human N-myc gene were introduced into the germline of transgenic mice, expression in some transgenic lines paralleled the tissue- and stage-specific expression of the endogenous murine gene. These findings demonstrate differences in the regulation of N-myc genes in recipient cells following in vitro versus in vivo introduction, suggesting that early developmental events may play a role in the regulation of N-myc expression.
Zimmerman
K
K
Howard Hughes Medical Institute, New York, New York.
Legouy
E
E
Stewart
V
V
Depinho
R
R
Alt
F W
FW
D000367:Age Factors; D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000254:growth & development; D005786:Gene Expression Regulation; D007668:Kidney / Q000254:growth & development; D051379:Mice; D008822:Mice, Transgenic; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger / Q000235:genetics; D011993:Recombinant Fusion Proteins; D014158:Transcription, Genetic; D014162:Transfection
D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins