Evidences for the existence of endothelium-derived relaxing factor in the renal medulla.
The basal levels of cGMP in renal medulla slices were enhanced when the slices were stimulated with both endothelium-dependent (acetylcholine) and endothelium-independent (molsidomine) vasodilators. When preincubated with NG-mono-methyl-L-arginine, a specific inhibitor of endothelium-derived relaxing factor, only the acetylcholine-stimulated increase was completely abolished. Furthermore, a preincubation with L-arginine, a selective precursor of endothelium-derived relaxing factor, enhanced the cGMP levels. The results indicate that the renal medulla, presumably the endothelial cells of the vasa recta, is able to produce endothelium-derived relaxing factor.
Biondi
M L
ML
Department of Physiology and Biophysics, Mayo School of Medicine, Mayo Clinic, Rochester, Minnesota 55905.
Dousa
T
T
Vanhoutte
P
P
Romero
J C
JC
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D006152:Cyclic GMP / Q000032:analysis / Q000378:metabolism*; D004285:Dogs; D005260:Female; D007679:Kidney Medulla / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008981:Molsidomine / Q000494:pharmacology; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011292:Premedication; D019323:omega-N-Methylarginine
D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D001120:Arginine; D008981:Molsidomine; D006152:Cyclic GMP; D000109:Acetylcholine