Cyclic AMP-dependent expression of the heavy neurofilament (NF-H) polypeptide in differentiating neuroblastoma cells.
Dibutyryl cyclic AMP (dbcAMP) treatment of Neuro 2A neuroblastoma cells induces cell differentiation and neurite outgrowth. Undifferentiated cells express the heavy neurofilament polypeptide (NF-H) at a low level. Following differentiation, there is a large increase in its expression due to an increase in the corresponding cellular mRNA. A similar increase is seen following forskolin treatment of the cells indicating that the increase in NF-H expression is cyclic AMP-dependent. The increase in mRNA is due to an increase in gene transcription as demonstrated by a nuclear run-off assay.
Breen
K C
KC
Department of Cellular and Molecular Sciences, St. George's Hospital Medical School, London, U.K.
Anderton
B H
BH
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002454:Cell Differentiation; D005786:Gene Expression Regulation / Q000187:drug effects*; D007381:Intermediate Filament Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009447:Neuroblastoma; D016900:Neurofilament Proteins; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism*
D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins; D003994:Bucladesine