Na(+)-Ca2+ exchange activity in central nerve endings. I. Ionic conditions that discriminate 45Ca2+ uptake through the exchanger from that occurring through voltage-operated Ca2+ channels.
Ca2+ entrance in central nerve endings can occur through voltage-operated Ca2+ channels and/or through the Na(+)-Ca2+ antiporter. The aim of the present study was to evaluate, in brain synaptosomes, the possible contribution of these two Ca2+ entrance pathways in the process of 45Ca2+ uptake elicited by different extracellular ionic conditions. The decrease in extracellular Na+ concentration from 145 mM to 95 mM and its concomitant substitution with complemental concentration of K+ (5-55 mM) caused an increase in 45Ca2+ uptake, whereas an equimolar concentration of choline (50 mM), although in the presence of the same Na+ concentration (95 mM), failed to stimulate 45Ca2+ uptake. Only when the extracellular Na+ concentration was further lowered from 95 mM to 0 mM and substituted with equivalent amounts of choline (50-145 mM) did a dose-dependent stimulation of 45Ca2+ uptake occur. In addition, when the lowering of the extracellular Na+ concentration from 95 mM to 0 mM was compensated for by K+ concentrations higher than 55 mM (55-150 mM), 45Ca2+ uptake was higher than that elicited by Na+ ion substitution with equimolar amounts (50-145 mM) of choline. The amount of 45Ca2+ uptake induced by 55 mM K+ did not differ either in Na(+)-preincubated or in Na(+)-depleted synaptosomes. Synaptosomal membrane potential, monitored with the potential-sensitive fluorescent dye bis-(1,3-diethyltiobarbiturate)trimethineoxonol, showed a progressive depolarization when extracellular K+ concentrations were raised from 5 to 150 mM, reaching a plateau at 55 mM extracellular K+ concentration, whereas when choline (145 mM) completely substituted for extracellular Na+ ions, synaptosomal membrane potential did not show any depolarization. Collectively, these results demonstrate that 45Ca2+ uptake induced by 55 mM K+ ions occurs selectively through voltage-operated Ca2+ channels, whereas, in choline-substituted media, starting from 70 mM choline, Ca2+ ions seemed to utilize the Na(+)-Ca2+ antiporter to penetrate into synaptosomes. In contrast, when extracellular K+ concentrations are raised above 55 mM, 45Ca2+ entrance may occur through two cumulative mechanisms, the opening of Ca2+ channels that are activated by high K(+)-induced depolarization and the activation of the Na(+)-Ca2+ antiporter, which follows the reduction of the transmembrane Na+ electrochemical gradient.(ABSTRACT TRUNCATED AT 400 WORDS)
Taglialatela
M
M
Institute of Pharmacology, 2nd School of Medicine, University of Naples, Italy.
Di Renzo
G
G
Annunziato
L
L
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000502:physiology*; D002352:Carrier Proteins / Q000502:physiology*; D002794:Choline / Q000494:pharmacology; D003342:Corpus Striatum / Q000378:metabolism; D008297:Male; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism*; D019831:Sodium-Calcium Exchanger; D013574:Synaptosomes / Q000378:metabolism; D013858:Thiobarbiturates
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D002352:Carrier Proteins; D019831:Sodium-Calcium Exchanger; D013858:Thiobarbiturates; C025284:bis(1,3-diethylthiobarbiturate)trimethineoxonol; D012964:Sodium; D002794:Choline; D011188:Potassium; D002118:Calcium