Heme-copper and heme-heme interactions in the cytochrome bo-containing quinol oxidase of Escherichia coli.
The cytochrome bo quinol oxidase of Escherichia coli is one of two respiratory O2 reductases which the bacterium synthesizes. The enzyme complex contains copper and 2 mol of b-type heme. Electron paramagnetic resonance (epr) spectroscopy of membranes from a strain having amplified levels of this enzyme complex reveals signals from low- and high-spin b-type hemes, but the copper, now established as a component of the oxidase, is not directly detectable by epr. The high-spin signal from the cytochrome bo complex, which we attribute to cytochrome o, when titrated potentiometrically, gives a bell-shaped curve. The low potential side of this curve is biphasic (Em7 approximately 180 and 280 mV) and corresponds to the reduction/oxidation of the cytochrome(s). The high potential side of the bell-shaped curve is monophasic (Em7 approximately 370 mV) and is proposed to be due to reduction/oxidation of a copper center which, when in the Cu(II) form, is tightly spin-coupled to a heme, probably cytochrome o, resulting in a net even spin system and loss of the epr spectrum. The low-spin cytochrome b titrates biphasically with Em7 values of approximately 180 and 280 mV, similar to the high-spin component but without the loss of signal at high potentials.
Salerno
J C
JC
Department of Biochemistry and Microbiology, University of St. Andrews, United Kingdom.
Bolgiano
B
B
Poole
R K
RK
Gennis
R B
RB
Ingledew
W J
WJ
D002248:Carbon Monoxide / Q000494:pharmacology; D002462:Cell Membrane / Q000201:enzymology; D003300:Copper / Q000378:metabolism*; D003573:Cytochrome b Group / Q000378:metabolism*; D003580:Cytochromes / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins; D006418:Heme / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*
D003573:Cytochrome b Group; D003580:Cytochromes; D029968:Escherichia coli Proteins; D006418:Heme; D003300:Copper; D002248:Carbon Monoxide; C017168:cytochrome bo, E coli; D010088:Oxidoreductases; C020094:duroquinol oxidase