title: Ocular avirulence of a herpes simplex virus type 1 strain is associated with heightened sensitivity to alpha/beta interferon.
abstract: BALB/c mice infected on the scarified cornea with herpes simplex virus type 1 strain 35 [HSV-1(35)] rarely developed ocular disease even at challenge doses as high as 10(7) PFU per eye. In contrast, HSV-1(RE) consistently induced stromal keratitis at an inoculum of 2 x 10(4) PFU. The goal of this study was to determine the reason for the difference in virulence between the two HSV strains. Both HSV-1 strains replicated to similar titers in excised corneal "buttons." However, after in vivo infection of the cornea, the growth of strain 35 was evident only during the first 24 h postinfection, whereas the replication of strain RE persisted for at least 4 days. In vitro tests revealed that HSV-1(35) was greater than 10 times more sensitive to alpha/beta interferon (IFN-alpha/beta) than HSV-1(RE). Both strains induced comparable serum levels of IFN after intraperitoneal inoculation. The kinetics of HSV-1(35) clearance from the eye was markedly altered by treatment with rabbit anti-IFN-alpha/beta. Virus titers exceeding 10(4) PFU per eye could be demonstrated 4 to 5 days postinfection in mice given a single inoculation of antiserum 1 h after infection. Furthermore, anti-IFN treatment in 3-week-old mice infected with HSV-1(35) led to the development of clinically apparent corneal disease which subsequently progressed to stromal keratitis in the majority of recipients. These results indicate that the striking difference in the capacity of HSV-1(35) and HSV-1(RE) to induce corneal disease was related to the inherently greater sensitivity of strain 35 to IFN-alpha/beta produced by the host in response to infection.
authors.affiliation: Department of Microbiology and Immunology, University of South Alabama, College of Medicine, Mobile 36688.
authors.forename: Y H
authors.initials: YH
authors.lastname: Su
authors.forename: J E
authors.initials: JE
authors.lastname: Oakes
authors.forename: R N
authors.initials: RN
authors.lastname: Lausch
mesh_terms: D000818:Animals; D003315:Cornea / Q000382:microbiology*; D005260:Female; D007106:Immune Sera; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000097:blood / Q000494:pharmacology*; D007635:Keratitis, Dendritic / Q000382:microbiology*; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D018139:Simplexvirus / Q000187:drug effects / Q000254:growth & development / Q000472:pathogenicity*; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects; D014774:Virulence
chemical_list: D007106:Immune Sera; D007370:Interferon Type I