Functional expression of Desulfovibrio vulgaris Hildenborough cytochrome c3 in Desulfovibrio desulfuricans G200 after conjugational gene transfer from Escherichia coli.
Plasmid pJRDC800-1, containing the cyc gene encoding cytochrome c3 from Desulfovibrio vulgaris subsp. vulgaris Hildenborough, was transferred by conjugation from Escherichia coli DH5 alpha to Desulfovibrio desulfuricans G200. The G200 strain produced an acidic cytochrome c3 (pI = 5.8), which could be readily separated from the Hildenborough cytochrome c3 (pI = 10.5). The latter was indistinguishable from cytochrome c3 produced by D. vulgaris subsp. vulgaris Hildenborough with respect to a number of chemical and physical criteria.
Voordouw
G
G
Department of Biological Sciences, University of Calgary, Alberta, Canada.
Pollock
W B
WB
Bruschi
M
M
Guerlesquin
F
F
Rapp-Giles
B J
BJ
Wall
J D
JD
D003227:Conjugation, Genetic; D003574:Cytochrome c Group / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D003901:Desulfovibrio / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005838:Genotype; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D014162:Transfection
D003574:Cytochrome c Group; D011994:Recombinant Proteins; C017165:cytochrome c(3)