Some aspects of fluorescence and microcalorimetric studies of cytochrome C oxidase.
Effects of the solubilizing detergent type, pH and temperature on the structure of cytochrome c oxidase have been studied by the intrinsic fluorescence and scanning microcalorimetry methods. The data obtained allow to conclude that the enzyme solubilization by lauryl maltoside gives a more native preparation in comparison with that obtained by solubilization in Tween 80.
Musatov
A
A
Laboratory of Biophysics, Institute of Experimental Physics, Slovac Acad. Sci., Kosice.
Permyakov
E A
EA
Bagelova
J
J
Morozova
L
L
Snyrov
V I
VI
D000818:Animals; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D003902:Detergents; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000378:metabolism*; D005960:Glucosides; D006863:Hydrogen-Ion Concentration; D011136:Polysorbates; D012995:Solubility; D013050:Spectrometry, Fluorescence; D013696:Temperature
D003902:Detergents; D005960:Glucosides; D011136:Polysorbates; C040358:dodecyl maltoside; D003576:Electron Transport Complex IV