Suckling inhibits release of luteinizing hormone-releasing hormone from the bovine median eminence following ovariectomy.
The effect of suckling on depletion of hypothalamic LHRH from the median eminence (ME) following ovariectomy (OVX) was determined in cattle. Multiparous, postpartum Holstein cows were assigned randomly to three groups: intact, nonsuckled (INT, n = 4); ovariectomized (3 to 5 d after parturition), nonsuckled (OVX, n = 4); and ovariectomized (3 to 5 d after parturition) and suckled by three calves (OVX-S, n = 5). Blood samples were collected at three periods (1 to 7 d before parturition and 3 to 5 d and 31 to 37 d after parturition) to determine plasma LH concentration. At 31 to 37 d after parturition, all cows were slaughtered and each ME was collected and mid-sagitally sectioned. The left half of each ME was used to determine content and concentration of LHRH. Concentrations of LH and LHRH were determined by RIA. Plasma LH concentration was similar among the three groups at 1 to 7 d before parturition and 3 to 5 d after parturition; however, at 31 to 37 d after parturition, OVX cows had a greater (P less than .05) concentration of LH (2.25 +/- .64 ng/ml) than either INT (.47 +/- .10 ng/ml) or OVX-S (.92 +/- .14 ng/ml) cows. Content of LHRH in the ME of INT (80.12 +/- 15.0 ng) and OVX-S 109.8 +/- 16.4 ng) cows was similar but was greater (P less than .05) than that in OVX cows (48.95 +/- 5.9 ng).(ABSTRACT TRUNCATED AT 250 WORDS)
Zalesky
D D
DD
Texas Agricultural Experiment Station, College Station.
Forrest
D W
DW
McArthur
N H
NH
Wilson
J M
JM
Morris
D L
DL
Harms
P G
PG
D000818:Animals; D000833:Animals, Suckling; D002417:Cattle / Q000378:metabolism*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000097:blood; D008321:Mammary Glands, Animal / Q000502:physiology*; D008473:Median Eminence / Q000378:metabolism*; D010052:Ovariectomy / Q000662:veterinary*; D011247:Pregnancy; D011897:Random Allocation
D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone