Virus-vs endotoxin-induced activation of liver macrophages.
The response of liver macrophages (Kupffer cells) to distinct pathogenic material was investigated by comparing virus- and endotoxin-induced macrophage activation. Endotoxin-induced stimulation and induction with Newcastle disease virus (NDV) or Sendai virus led to the release of the same pattern of prostanoids characterized by a predominant production of prostaglandin E2 (PGE2). With respect to peptide mediators, hepatic macrophages secreted tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 after viral induction and endotoxin treatment, respectively. In response to viruses, however, much more interleukin-6 and TNF-alpha was detected than after endotoxin stimulation. Interferon type I (interferon-alpha/beta), on the other hand, was only detected in the supernatants of macrophages infected with viruses, but not of those exposed to endotoxin. This study also revealed that rat TNF-alpha exists in several soluble species, some of which are glycosylated.
Busam
K J
KJ
Biochemisches Institut, Universität Freiburg, Federal Republic of Germany.
Homfeld
A
A
Zawatzky
R
R
Kästner
S
S
Bauer
J
J
Gerok
W
W
Decker
K
K
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D015232:Dinoprostone / Q000096:biosynthesis; D007163:Immunosorbent Techniques; D007370:Interferon Type I / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D007700:Kinetics; D007728:Kupffer Cells / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008262:Macrophage Activation / Q000502:physiology*; D008297:Male; D009522:Newcastle disease virus / Q000502:physiology*; D010222:Parainfluenza Virus 1, Human / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D012475:Salmonella; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D007370:Interferon Type I; D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D015232:Dinoprostone