Alpha-latrotoxin triggers an increase of ionized calcium in Xenopus oocytes injected with rat brain mRNA.
When Xenopus oocytes are injected with rat brain mRNA, they acquire the ability to respond to bath applied alpha-latrotoxin. This spider venom toxin is normally highly selective for nerve endings, where its binding is associated with a high-frequency, quantal discharge of neurotransmitter. By 'transplanting' toxin acceptor sites to Xenopus oocytes, we have observed both a toxin-mediated rise in cellular ionized Ca along with the triggering of a calcium-dependent chloride channel in these cells. This approach may contribute both to a better understanding of the mechanism of action of this toxin and to efforts to clone the cDNA for this binding site.
Umbach
J A
JA
Department of Pharmacology, UCLA School of Medicine 90024-1735.
Grasso
A
A
Gundersen
C B
CB
D000818:Animals; D001180:Arthropod Venoms / Q000494:pharmacology*; D001921:Brain / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism* / Q000502:physiology; D005260:Female; D008564:Membrane Potentials / Q000187:drug effects; D009498:Neurotoxins / Q000494:pharmacology*; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism* / Q000502:physiology; D051381:Rats; D013111:Spider Venoms / Q000494:pharmacology*; D014982:Xenopus laevis
D001180:Arthropod Venoms; D009498:Neurotoxins; D012333:RNA, Messenger; D013111:Spider Venoms; C020763:alpha-latrotoxin; D002118:Calcium