Detection of microbial trypsin-like enzymes by use of an agar gel.
Certain oral micro-organisms associated with periodontal diseases elaborate trypsin-like enzymes. This study describes a single assay for these enzymes, based on the incorporation of the synthetic substrate N-alpha-benzoyl-DL-arginine-p-nitroanilide (BAPNA) into ion-agar gel. The system can provide quantitative or qualitative data within 24 h and is suitable for use in the dental clinic.
Pederson
E D
ED
Naval Dental Research Institute, Great Lakes, Illinois 60088-5259.
Lamberts
B L
BL
D001120:Arginine / Q000031:analogs & derivatives; D001439:Bacteroides / Q000201:enzymology*; D001586:Benzoylarginine Nitroanilide; D002206:Capnocytophaga / Q000201:enzymology*; D003773:Dental Plaque / Q000382:microbiology; D006801:Humans; D009055:Mouth / Q000382:microbiology; D010518:Periodontitis / Q000382:microbiology*; D014357:Trypsin / Q000032:analysis*
D001586:Benzoylarginine Nitroanilide; D001120:Arginine; D014357:Trypsin