Vasopressin gene expression is stimulated by cyclic AMP in homologous and heterologous expression systems.
The possible role of cyclic AMP (cAMP) in the regulation of the vasopressin (VP) gene was tested in two cellular expression systems: one cell line with endogenous VP expression and the other which was transiently with a VP promoter-luciferase fusion gene. 8,Bromo-cAMP stimulated the VP mRNA content about 4-fold in the human VP-expressing small cell lung carcinoma cell line GLC-8. The luciferase activity in P19 embryonal carcinoma cells which were transiently transfected with -174 to +44 of the 5'-flanking region of the human VP gene linked to the firefly luciferase gene, was stimulated about 2-fold by the cAMP analogue. The results indicate that cAMP plays a role in the upregulation of the VP gene and hence point to several putative nucleotide motives in the promoter functionally conferring this response.
Verbeeck
M A
MA
Rudolf Magnus Institute, Medical Faculty, University of Utrecht, The Netherlands.
Adan
R A
RA
Burbach
J P
JP
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000199:Actins / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D018288:Carcinoma, Small Cell; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000494:pharmacology*; D004274:DNA, Recombinant; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008175:Lung Neoplasms; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D014162:Transfection; D014407:Tumor Cells, Cultured; D014667:Vasopressins / Q000235:genetics*
D000199:Actins; D004274:DNA, Recombinant; D012333:RNA, Messenger; D014667:Vasopressins; D015124:8-Bromo Cyclic Adenosine Monophosphate; D000242:Cyclic AMP; D008156:Luciferases