Neoplastic transformation is associated with coordinate induction of nuclear and cytoplasmic oxidative phosphorylation genes.
Neoplastic transformation was found to have a marked effect on the expression of nuclear DNA (nDNA)- and mitochondrial DNA (mtDNA)-encoded oxidative phosphorylation (OXPHOS) genes. Examining three pairs of human diploid fibroblasts and their SV 40-transformed counterparts revealed that mRNAs for the nuclear-encoded ATP synthase beta and the adenine nucleotide translocator (ANT) isoform 1 and 2 genes were markedly induced, whereas the mRNA for the ANT isoform 3 gene remained unchanged. The mRNA levels for the mtDNA-encoded 12 S rRNA, ND2, ATPase6+8, COIII, ND5+6, and Cytb genes were also increased, whereas the mtDNA number declined. Similar analysis of a cervical carcinoma (HeLa), fibrosarcoma (HT1080), and an Epstein-Barr virus (EBV)-transformed lymphoblastoid line (EBV-L) revealed that all three ANT isoforms were also expressed in these cells. Hence, changes in the expression of OXPHOS genes may be a common feature of transformed cells.
Torroni
A
A
Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322.
Stepien
G
G
Hodge
J A
JA
Wallace
D C
DC
D001483:Base Sequence; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic; D003593:Cytoplasm / Q000378:metabolism; D015342:DNA Probes; D004272:DNA, Mitochondrial / Q000235:genetics*; D006801:Humans; D000226:Mitochondrial ADP, ATP Translocases / Q000235:genetics*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010085:Oxidative Phosphorylation; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013539:Simian virus 40 / Q000235:genetics*; D014158:Transcription, Genetic
D015342:DNA Probes; D004272:DNA, Mitochondrial; D015345:Oligonucleotide Probes; D000226:Mitochondrial ADP, ATP Translocases