title: [The role of inner membrane in the realization of cAMP-dependent activation of mitochondrial enzymes].
abstract: It was shown that the increase in the activities of transhydrogenase and NAD(+)-dependent isocitrate dehydrogenase after incubation of mitochondria with cAMP is due to the stimulating effect of cAMP on mitochondria, but not to the increased stability of mitochondria to the incubation procedure. Treatment of mitochondria with trypsin prevents the action of cAMP on the both enzymes. The integrity of the inner mitochondrial membrane is necessary for the manifestation of cAMP effect. Pretreatment of mitochondria with the local anesthetic, lidocaine, prevents the activation of NAD(P)(+)-transhydrogenase and NAD(+)-dependent isocitrate dehydrogenase during subsequent incubation of mitochondria with cAMP. It is concluded that the role of the inner mitochondrial membrane consists in the reception of the cAMP signal for the internal compartment of mitochondria, i.e. for mitoplasts. Peripheral protein(s) on the external side of the inner mitochondrial membrane seems to play a role in cAMP reception.
authors.forename: A E
authors.initials: AE
authors.lastname: Medvedev
authors.forename: L V
authors.initials: LV
authors.lastname: Trufanova
authors.forename: A V
authors.initials: AV
authors.lastname: Golubenko
authors.forename: V I
authors.initials: VI
authors.lastname: Kulinskiĭ
mesh_terms: D000818:Animals; D002729:Chloromercuribenzoates / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology; D004789:Enzyme Activation; D005260:Female; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000201:enzymology*; D007521:Isocitrate Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008012:Lidocaine / Q000494:pharmacology; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D009250:NADP Transhydrogenases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014357:Trypsin / Q000494:pharmacology
chemical_list: D002729:Chloromercuribenzoates; D008012:Lidocaine; D000242:Cyclic AMP; D007521:Isocitrate Dehydrogenase; D009247:NADH, NADPH Oxidoreductases; D009250:NADP Transhydrogenases; D014357:Trypsin
vernacular_title: Znachenie vnutrenneĭ membrany v realizatsii cAMP-zavisimoĭ aktivatsiifermentov mitokhondriĭ.