Activation process of macrophages after in vitro treatment of mouse lymphocytes with dodecylglycerol.
Alkylglycerols, inflammation products of cancerous membrane lipids, efficiently activate macrophages. A brief in vitro treatment (30 min) of peritoneal cells (mixture of non-adherent and adherent cells) with a small amount (50 ng/ml) of synthetic dodecylglycerol (DDG) resulted in greatly enhanced Fc-receptor-mediated ingestion activity of macrophages. However, treatment of adherent cells (macrophages) alone with DDG produced no significant enhancement of macrophage ingestion activity, implying that macrophage activation requires a contribution of non-adherent cells. DDG-treated non-adherent cells were found to generate a macrophage-activating signal factor. Studies with a serum free-0.1% egg albumin-supplemented RPMI 1640 medium revealed that a serum factor is essential for macrophage activation process. Time course analysis of stepwise transfers of conditioned media of DDG-treated or untreated B cells and T cells revealed that DDG-treated B cells rapidly transmit a factor to untreated T cells which yield the ultimate macrophage-activating factor. This signal transmission among these cells for the macrophage activation process is too rapid to allow time for synthesis of inducible gene products. Thus, we hypothesized that a serum factor is modified by the pre-existing function of DDG-treated B cells and further modified by the pre-existing function of untreated T cells to yield macrophage-activating factor. This hypothesis was confirmed by the demonstration that DDG-treated splenic non-adherent cell ghosts modify a serum factor to yield macrophage-activating factor.
Homma
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S
Department of Microbiology and Immunology, Hahnemann University School of Medicine, Philadelphia, PA 19102.
Yamamoto
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N
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D002450:Cell Communication / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D005989:Glycerides / Q000494:pharmacology*; D007848:Laurates / Q000494:pharmacology*; D007850:Lauric Acids / Q000494:pharmacology*; D008222:Lymphokines / Q000096:biosynthesis; D008262:Macrophage Activation / Q000276:immunology*; D016215:Macrophage-Activating Factors; D051379:Mice; D008807:Mice, Inbred BALB C; D050178:Monoglycerides; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
D005989:Glycerides; D007848:Laurates; D007850:Lauric Acids; D008222:Lymphokines; D016215:Macrophage-Activating Factors; D050178:Monoglycerides; C020777:monolaurin