Evidence for one-electron oxidation of benzylpenicillin G by lactoperoxidase compounds I and II.
The ability of the widely used antibiotic benzylpenicillin G to undergo a peroxidatic oxidation to produce free radical species has been investigated using conventional spectrophotometry, rapid scan spectrophotometry and an ESR spin trapping technique. Lactoperoxidase was used as a model for a drug oxidizing system. The results of the investigation indicate a normal peroxidatic pathway of benzylpenicillin degradation which leads to superoxide radical generation.
Metodiewa
D
D
Department of Chemistry, University of Alberta, Edmonton, Canada.
Dunford
H B
HB
D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D006861:Hydrogen Peroxide; D066298:In Vitro Techniques; D007784:Lactoperoxidase / Q000378:metabolism*; D010084:Oxidation-Reduction; D010400:Penicillin G / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism*; D013057:Spectrum Analysis; D013482:Superoxide Dismutase / Q000378:metabolism
D005609:Free Radicals; D006861:Hydrogen Peroxide; D007784:Lactoperoxidase; D010544:Peroxidases; D013482:Superoxide Dismutase; D010400:Penicillin G