An antigen chimera of poliovirus induces antibodies against human papillomavirus type 16.
It has been established that the surface of poliovirus type 1 can be extensively modified to incorporate antigenic domains from other poliovirus serotypes and from unrelated viruses. The fact that the modified (chimeric) viruses exhibit dual antigenicity and immunogenicity led us to explore the possibility of using the Sabin vaccine strain of poliovirus type 1 as a vector for the presentation of antigenic domains from human papillomavirus type 16 (HPV-16), a virus associated with the development of cervical carcinoma. We report here the construction and characterization of a chimeric poliovirus containing a 16-residue sequence derived from the major capsid protein (L1) of HPV-16. This virus chimera stimulated the production in rabbits of antibodies which recognized the HPV-16-derived peptide and an L1 fusion protein synthesized in Escherichia coli and detected HPV-16 in human biopsy material by immunoperoxidase staining. The possibility that poliovirus-HPV chimeras could be used as vaccines against HPV-16 is discussed.
Jenkins
O
O
United Medical School, University of Reading, United Kingdom.
Cason
J
J
Burke
K L
KL
Lunney
D
D
Gillen
A
A
Patel
D
D
McCance
D J
DJ
Almond
J W
JW
D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000276:immunology*; D000917:Antibody Formation; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D002460:Cell Line; D002678:Chimera; D003062:Codon / Q000235:genetics; D006801:Humans; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D027383:Papillomaviridae / Q000235:genetics / Q000276:immunology*; D017955:Poliovirus / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D014779:Virus Replication
D000914:Antibodies, Viral; D000956:Antigens, Viral; D003062:Codon