Specific detection of enteroviruses in clinical samples by molecular hybridization using poliovirus subgenomic riboprobes.
Enteroviruses were specifically detected in crude clinical specimens or in cell cultures in which the viruses were amplified by dot hybridization by using poliovirus type 1-derived, subgenomic radiolabeled cRNA probes (riboprobes). The sensitivity of this test varied from 2.5 to 33%, when clinical specimens without cell culture were examined, and was about 85% in cell culture lysates. The specificity of the test was 90 to 100%. The riboprobe corresponding to the 5'-noncoding sequence specifically detected the majority of enteroviruses (56 of 57 tested); the riboprobe derived from the VPI capsid region hybridized with the three poliovirus serotypes and with some coxsackieviruses type A and with echovirus type 7. Echovirus 22 did not hybridize with any riboprobe. In stool specimens, nasal aspirates, and cerebrospinal fluids from patients with meningitis, only one type of virus was identified in different clinical samples from the same patient by the seroneutralization test. Hybridization allowed the detection of enteroviral RNAs easily in stool specimens and nasal aspirates but with a low efficiency in cerebrospinal fluids without amplification of the viruses in cell cultures.
Petitjean
J
J
Laboratoire de Virologie, Centre Hospitalier Régional Universitaire de Caen, France.
Quibriac
M
M
Freymuth
F
F
Fuchs
F
F
Laconche
N
N
Aymard
M
M
Kopecka
H
H
D002213:Capsid / Q000235:genetics; D036022:Capsid Proteins; D004770:Enterovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D029822:Enterovirus B, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D004769:Enterovirus Infections / Q000382:microbiology; D006801:Humans; D008587:Meningitis, Viral / Q000382:microbiology; D015336:Molecular Probe Techniques; D009693:Nucleic Acid Hybridization; D017955:Poliovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D015347:RNA Probes; D012703:Serotyping
D036022:Capsid Proteins; D015347:RNA Probes; C442540:VP1 protein, Poliovirus