Suppression of herpes simplex virus type 1 reactivation from latency by (+-)-9-([(Z)-2-(hydroxymethyl)cyclohexyl]methyl) guanine (L-653,180) in vitro.
Latent herpes simplex virus type 1 (HSV-1) infection was induced in human embryonic lung cells in vitro by using a combination of viral replication inhibitors and elevated temperature. Under reactivating conditions (superinfection by human cytomegalovirus or temperature manipulation), a nonantiviral thymidine kinase inhibitor (L-653,180) was found to suppress or delay reactivation of HSV-1 from latently infected human embryonic lung cells. L-653,180 alone or in combination with interferon was ineffective as a primary or acute viral replication inhibitor and was unable to induce latent HSV-1 infection in cell culture. These data suggest that initial or acute virus replication and replication resulting from reactivation from latency are separate events.
Nsiah
Y A
YA
Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, Hershey 17033.
Tolman
R L
RL
Karkas
J D
JD
Rapp
F
F
D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D005260:Female; D006147:Guanine / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D011247:Pregnancy; D018139:Simplexvirus / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D015163:Superinfection; D014775:Virus Activation / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; C065860:9-((2-(hydroxymethyl)cyclohexyl)methyl)guanine; D006147:Guanine