Cyclic nucleotide phosphodiesterases and methyltransferases in purified lymphocytes, monocytes and polymorphonuclear leucocytes from healthy donors and asthmatic patients.
Cyclic nucleotide phosphodiesterase and phospholipid N-methyl-transferase activities were simultaneously measured in purified polymorphonuclear cell-, mononuclear cell-, lymphocyte- and monocyte-homogenates from control subjects, from patients with atopic asthma and from patients with non-atopic asthma. Whereas cyclic AMP and cyclic GMP phosphodiesterase activities were found to be about 10-fold lower in polymorphonuclear than in mononuclear cells, phospholipid N-methyltransferase proved to be rather similar in each cell type from control donors. Cyclic AMP phosphodiesterase and phospholipid N-methyltransferase were significantly decreased in polymorphonuclear cells and monocytes from asthmatic patients compared with the control group while cyclic GMP phosphodiesterase was significantly impaired only in the monocyte subpopulation.
Prigent
A F
AF
Unité 205 INSERM, Institut National des Sciences appliquées de Lyon, France.
Fonlupt
P
P
Dubois
M
M
Némoz
G
G
Timouyasse
L
L
Pacheco
H
H
Pacheco
Y
Y
Biot
N
N
Perrin-Fayolle
M
M
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000097:blood*; D000293:Adolescent; D000328:Adult; D001249:Asthma / Q000201:enzymology*; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000201:enzymology; D008214:Lymphocytes / Q000201:enzymology; D008297:Male; D008780:Methyltransferases / Q000097:blood*; D008875:Middle Aged; D009000:Monocytes / Q000201:enzymology; D009504:Neutrophils / Q000201:enzymology; D050918:Phosphatidylethanolamine N-Methyltransferase
D008780:Methyltransferases; D050918:Phosphatidylethanolamine N-Methyltransferase; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases