title: Characterization of herpes simplex virus type 2 transcription during latent infection of mouse trigeminal ganglia.
abstract: Using a cornea trigeminal ganglion model, we have investigated transcription by herpes simplex virus type 2 (HSV-2) during latency in mice. Latency was verified 2 months postinoculation by reactivation of HSV-2 after explant cocultivation of trigeminal ganglia from the majority of mice (83%). Transcription during latent HSV-2 infection was limited to the repeat regions of the viral genome as determined by in situ hybridization using restriction fragment probes representing 100% of the HSV-2 genome. Further mapping of the positively hybridizing region by using subfragments showed that transcription occurred from approximately 11.5 kb of contiguous DNA fragments. A 1.0-kb PvuI-BamHI fragment within the BamHI F fragment and a 0.3-kb BamHI-SalI fragment and a 3.4-kb SalI-BamHI fragment within the BamHI P fragment hybridized more strongly than other subfragments in in situ hybridization experiments. All positive signals were confined to the nucleus. The RNA that hybridized to the 3.4-kb SalI-BamHI DNA fragment probe by in situ hybridization corresponded to a 2.3-kb transcript on Northern (RNA) blots. Under our conditions for Northern blot hybridization, the 3.4-kb SalI-BamHI probe of HSV-2 hybridized to a limited degree with the latency-associated transcripts of HSV-1. Shorter spliced species of latency-associated transcript RNA, which are seen during HSV-1 latency, have not been detected in latent HSV-2 RNA. However, viral gene expression during HSV-2 latency appears to be very similar to that during HSV-1 latency.
authors.affiliation: Wistar Institute, Philadelphia, Pennsylvania 19104-4268.
authors.forename: W J
authors.initials: WJ
authors.lastname: Mitchell
authors.forename: S L
authors.initials: SL
authors.lastname: Deshmane
authors.forename: A
authors.initials: A
authors.lastname: Dolan
authors.forename: D J
authors.initials: DJ
authors.lastname: McGeoch
authors.forename: N W
authors.initials: NW
authors.lastname: Fraser
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D046508:Culture Techniques; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D051379:Mice; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D018139:Simplexvirus / Q000235:genetics* / Q000254:growth & development; D014158:Transcription, Genetic; D012668:Trigeminal Ganglion / Q000382:microbiology
chemical_list: D015342:DNA Probes; D004279:DNA, Viral; D012367:RNA, Viral