title: The uptake of phosphatidylcholine by small intestinal brush border membrane is protein-mediated.
abstract: Brush border membrane vesicles prepared from rabbit small intestine are essentially free of basolateral membranes and nuclear, mitochondrial, microsomal and cytosolic contaminants. The resulting brush border membrane is unstable due to intrinsic lipases and proteinases. The PC transfer between small unilamellar lipid vesicles or mixed lipid micelles as the donor and the brush border membrane vesicles as the acceptor is protein-mediated. After proteolytic treatment of brush border membrane with papain or proteinase K the PC transfer activity is lost and the kinetics of PC uptake are similar to those measured with erythrocytes under comparable conditions. Evidence is presented to show that the PC transfer activity resides in the apical membrane of the enterocyte and not in the basolateral part of the plasma membrane. Furthermore, the activity is localized on the external surface of the brush border membrane exposed to the aqueous medium with its active centre probably not in direct contact with the lipid bilayer of the membrane. Proteins released from brush border membrane by proteolytic treatment catalyze PC exchange between different populations of small unilamellar vesicles. Furthermore, these protein(s) bind(s) PC forming a PC-protein complex.
authors.affiliation: Laboratorium für Biochemie, Eigenössische Technische Hochschule Zürich, Switzerland.
authors.forename: H
authors.initials: H
authors.lastname: Thurnhofer
authors.forename: H
authors.initials: H
authors.lastname: Hauser
mesh_terms: D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism*; D007700:Kinetics; D008871:Microvilli / Q000378:metabolism*; D010206:Papain; D010713:Phosphatidylcholines / Q000378:metabolism*; D011506:Proteins / Q000502:physiology*; D011817:Rabbits; D013816:Thermodynamics
chemical_list: D010713:Phosphatidylcholines; D011506:Proteins; D010206:Papain