Identification of the cross-reactive antigens between Marek's disease virus and pseudorabies virus by monoclonal antibodies.
Among the 33 monoclonal antibodies (MAbs) against pseudorabies virus (PRV) examined, three MAbs (24-17, 74-26, and 8) were found to react with cells infected with Marek's disease virus (MDV)-related viruses by immunofluorescence test. Two of the MAbs (24-17 and 74-26) reacted with the nuclei of cells infected with MDV serotype 1 (MDV1), MDV serotype 2 (MDV2), and herpesvirus of turkeys (HVT), whereas MAb 8 reacted with the cytoplasm of MDV2- and HVT-infected cells. However, none of the MAbs against MDV1, MDV2, and HVT that were examined reacted with PRV-infected cells. None of these three MAbs against PRV reactive with MDV-related viruses cross-reacted with the cells infected with other herpesviruses, such as herpes simplex virus type 1, herpes simplex virus type 2, varicella zoster virus, Epstein-Barr virus, or human herpesvirus 6. Southern-blot hybridization under stringent or less-stringent conditions showed that no significant DNA homology was detected between PRV DNA and MDV DNA.
Nakajima
K
K
Department of Pathology, Osaka University, Japan.
Kweon
C H
CH
An
S H
SH
Shibayama
T
T
Wakamiya
N
N
Kato
S
S
Hirai
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D015139:Blotting, Southern; D002460:Cell Line; D002478:Cells, Cultured; D003429:Cross Reactions; D004279:DNA, Viral / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006564:Herpesviridae / Q000235:genetics / Q000276:immunology; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000276:immunology*; D008381:Herpesvirus 2, Gallid / Q000235:genetics / Q000276:immunology*; D006801:Humans; D006825:Hybridomas
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D004279:DNA, Viral