title: Antibodies bind and activate beta adrenergic and cholinergic lymphocyte receptors in Chagas' disease.
abstract: It is demonstrated that murine chagasic IgG and the corresponding F(ab')2 fragments interfere with beta adrenergic and muscarinic cholinergic specific ligand occupancy on T cell-enriched population. From the interaction between chagasic IgG or F(ab')2 with Lyt-1+ cells, an increase in cAMP levels occurs as a consequence of beta adrenergic receptor activation. On the contrary, chagasic IgG or F(ab')2 interactions with Lyt-2+ cells induce an activation of muscarinic cholinergic receptor, leading to an increment in cGMP. Muscarinic cholinergic and beta adrenergic stimulation trigger the release of PGE2 and TXB2, respectively. Lyt-2+ cells treated with chagasic IgG or F(ab')2 are able to decrease the contractility of mouse atria. The same negative inotropic effect is elicited with Lyt-2+ cells from Trypanosoma cruzi-infected mice susceptible to developed myocarditis. The implications of these results in the pathogenesis of Chagas' myocarditis are discussed.
authors.affiliation: Centro de Estudios Farmacológicos y de Principios Naturales, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
authors.forename: G
authors.initials: G
authors.lastname: Gorelik
authors.forename: A M
authors.initials: AM
authors.lastname: Genaro
authors.forename: L
authors.initials: L
authors.lastname: Sterin-Borda
authors.forename: S
authors.initials: S
authors.lastname: Gonzalez Cappa
authors.forename: E S
authors.initials: ES
authors.lastname: Borda
mesh_terms: D000818:Animals; D016275:Atrial Function; D014355:Chagas Disease / Q000097:blood*; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D007140:Immunoglobulin Fab Fragments / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism*; D008214:Lymphocytes / Q000648:ultrastructure*; D051379:Mice; D008807:Mice, Inbred BALB C; D009200:Myocardial Contraction / Q000187:drug effects; D011485:Protein Binding; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D011950:Receptors, Cholinergic; D013601:T-Lymphocytes / Q000378:metabolism; D013929:Thromboxane B2 / Q000378:metabolism
chemical_list: D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D011943:Receptors, Adrenergic, beta; D011950:Receptors, Cholinergic; D013929:Thromboxane B2; D000242:Cyclic AMP; D006152:Cyclic GMP; D015232:Dinoprostone