Effect of trifluoperazine and colchicine on smooth muscle cellular proliferative and secretory activity induced by hypercholesterolemic medium in vitro.
The present study, addressed to understand the mechanism behind the cholesterol-induced proliferative and collagen secretory activity of smooth muscle cells, revealed that cholesterol-induced smooth muscle cellular DNA synthesis and collagen secretion was mediated through its ability to amplify the intracellular cGMP signal because of the fact that Trifluoperazine (an anticalmodulin and blocker of phospholipase A2) and colchicine (an antitubulin and inhibitor of guanyl cyclase) inhibited DNA synthesis and collagen-secretory activity of smooth muscle cells by their ability to decrease the cGMP levels within smooth muscle cells. From these results we suggest that membrane cholesterol modulated phospholipase 'A2' activity may be the basic mechanism involved in cholesterol-induced proliferative and collagen-secretory activity of smooth muscle cells in vitro.
Mehta
U M
UM
Department of Experimental Medicine, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Kaul
D
D
D000818:Animals; D001011:Aorta; D002455:Cell Division / Q000187:drug effects; D002784:Cholesterol / Q000494:pharmacology*; D003078:Colchicine / Q000494:pharmacology*; D003094:Collagen / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D011817:Rabbits; D014268:Trifluoperazine / Q000494:pharmacology*
D014268:Trifluoperazine; D003094:Collagen; D004247:DNA; D002784:Cholesterol; D010741:Phospholipases A; D054467:Phospholipases A2; D006152:Cyclic GMP; D003078:Colchicine