Identification of alpha 2- and alpha 3-subunits of the GABAA-benzodiazepine receptor complex purified from the brains of young rats.
Polyclonal antibodies were raised to synthetic amino acid sequences of the bovine GABAA receptor alpha 2- and alpha 3-subunits and purified by affinity chromatography on a column coupled with the respective peptide. Anti-peptide alpha 2(416-424) and anti-peptide alpha 3(459-467) antibodies immunoprecipitated GABAA receptors and recognized a protein of 53 kDa (P53) and 59 kDa (P59), respectively, in Western blots of GABAA receptors purified from the brains of 5-10 day old rats. P53 as well as P59 are specifically photolabeled by [3H]flunitrazepam and are recognized by the alpha-subunit specific monoclonal antibody bd 28.
Fuchs
K
K
Department of Biochemical Psychiatry, Psychiatrische Universitätsklinik Wien, Vienna, Austria.
Adamiker
D
D
Sieghart
W
W
D000818:Animals; D015153:Blotting, Western; D001923:Brain Chemistry; D002846:Chromatography, Affinity; D005445:Flunitrazepam / Q000378:metabolism; D007163:Immunosorbent Techniques; D011487:Protein Conformation; D051381:Rats; D011963:Receptors, GABA-A / Q000302:isolation & purification* / Q000378:metabolism
D011963:Receptors, GABA-A; D005445:Flunitrazepam