Effect of sialoadenectomy on ethanol-induced gastric mucosal damage in the rat: role of neutrophils.
We have observed that removal of the salivary glands is associated with an increase in the susceptibility to gastric mucosal damage in the rat. In the present study, we have examined the effect of sialoadenectomy on ethanol-induced mucosal hemorrhagic damage and myeloperoxidase (MPO) activity. Hemorrhagic damage and MPO activity in response to intragastric 50% w/v ethanol were greater in sialoadenectomized rats when compared with sham-operated animals. Pretreatment with 16,16-dimethylprostaglandin E2 (0.3 micrograms/kg s.c.) reduced damage and MPO activity in both sialoadenectomized and sham control rats receiving 50% ethanol. The reduction in these parameters was greater in control than in sialoadenectomized rats. Pretreatment with epidermal growth factor (5 micrograms/kg s.c.) significantly reduced MPO activity but did not significantly affect the extent of damage. These data suggest that sialoadenectomy is associated with an increase in mucosal inflammation in animals given ethanol. However, in some situations tissue inflammation (as indicated by MPO activity) was reduced, while the proportion of gastric mucosa exhibiting hemorrhagic damage was not changed.
Tepperman
B L
BL
Department of Physiology, Faculty of Medicine, University of Western Ontario, London, Canada.
Soper
B D
BD
D015064:16,16-Dimethylprostaglandin E2 / Q000494:pharmacology; D000818:Animals; D015232:Dinoprostone / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D000431:Ethanol / Q000633:toxicity*; D006471:Gastrointestinal Hemorrhage / Q000139:chemically induced / Q000503:physiopathology; D008297:Male; D009504:Neutrophils / Q000502:physiology*; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012469:Salivary Glands / Q000502:physiology*; D013276:Stomach Ulcer / Q000139:chemically induced / Q000201:enzymology / Q000503:physiopathology*
D000431:Ethanol; D004815:Epidermal Growth Factor; D009195:Peroxidase; D015232:Dinoprostone; D015064:16,16-Dimethylprostaglandin E2