[3H]D-serine labels strychnine-insensitive glycine recognition sites of rat central nervous system.
In the central nervous system, glycine binds to two recognition sites; one of them (G2), associated with the glutamate receptor, is insensitive to strychnine. Strychnine-insensitive sites were predominant in the forebrain areas and bound D-serine and D-alanine better than the respective L stereoisomers. [3H]D-serine was a more selective radioligand than [3H]glycine for the strychnine-insensitive sites. In the forebrain, the binding of both ligands was inhibited by the putative G2 receptor antagonists, 7-chlorokynurenate and 3-amino-1-hydroxy-2-pyrrolidone, while in pons and in spinal cord only the latter drug was effective. This may indicate the heterogeneity of strychnine-insensitive glycine recognition sites.
Danysz
W
W
Fidia-Georgetown Institute for the Neurosciences, Georgetown University School of Medicine, Washington, D.C. 20007.
Fadda
E
E
Wroblewski
J T
JT
Costa
E
E
D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism*; D005998:Glycine / Q000378:metabolism*; D007700:Kinetics; D011149:Pons / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D017470:Receptors, Glutamate; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000378:metabolism*; D012694:Serine / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism*; D013331:Strychnine / Q000378:metabolism / Q000494:pharmacology*; D014316:Tritium
D005971:Glutamates; D017470:Receptors, Glutamate; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter; D014316:Tritium; D012694:Serine; D013331:Strychnine; D005998:Glycine