title: Evaluation of pathogenicity and protective efficacy of serotype 2 Marek's disease virus from birds belonging to genus Gallus in Japan.
abstract: The new cloned serotype 2 Marek's disease viruses (MDV) of ML-6, ML-9, and ML-22 strains were inoculated in specific-pathogen-free (SPF) chicks to evaluate the pathogenicity and protective efficacy. Chicks inoculated or contact-infected with ML strains showed no gross and histological lesions in lymphoid organs, sciatic plexuses and other visceral organs during 10 weeks of observation periods, indicating that the viruses were non-pathogenic. Moreover, the viruses were found to be spread horizontally among chicks by demonstrating the presence of viremia in contacted chicks at 2 weeks-old. Chicks vaccinated with ML-6 at one day-old were protected against subsequent challenge by inoculation with virulent MDV strain of Md/5 at 4 or 7 days old or by contact infection at 7 days old with chickens previously inoculated with the same strain.
authors.affiliation: Department of Epizootiology, Hokkaido University, Sapparo, Japan.
authors.forename: J A
authors.initials: JA
authors.lastname: Lin
authors.forename: H
authors.initials: H
authors.lastname: Kodama
authors.forename: C
authors.initials: C
authors.lastname: Itakura
authors.forename: M
authors.initials: M
authors.lastname: Onuma
authors.forename: T
authors.initials: T
authors.lastname: Mikami
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D008381:Herpesvirus 2, Gallid / Q000302:isolation & purification / Q000472:pathogenicity*; D007564:Japan; D008380:Marek Disease / Q000209:etiology / Q000517:prevention & control*; D013045:Species Specificity; D013047:Specific Pathogen-Free Organisms; D014611:Vaccination / Q000662:veterinary*; D014774:Virulence