The effects of bFGF, IGF-I, and TGF-beta on RMo skeletal muscle cell proliferation and differentiation.
A new skeletal muscle cell line, rat myoblast omega or RMo, has been characterized with regard to the effects of three growth factors: basic fibroblast growth factor (bFGF), insulin-like growth factor I (IGF-I), and transforming growth factor beta (TGF-beta). Results indicate a differential response of these factors on both cell proliferation and differentiation. Exposure to bFGF and IGF-I stimulate proliferation, while TGF-beta has no effect on cell number. RMo cell differentiation, as indicated by skeletal myosin synthesis, is enhanced by IGF-I, whereas both bFGF and TGF-beta suppress differentiation. These responses are in agreement with the effects of bFGF, IGF-I, and TGF-beta on myogenic cells cultured from fetal and postnatal muscle, thereby suggesting that RMo cells can serve as a model system for the study of growth factor effects on skeletal muscle cells.
Johnson
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Department of Animal Sciences, University of Arizona, Tucson 85721.
Allen
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D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D007124:Immunoenzyme Techniques; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007700:Kinetics; D009132:Muscles / Q000166:cytology* / Q000187:drug effects; D051381:Rats; D013002:Somatomedins / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism; D015291:Transforming Growth Factors / Q000494:pharmacology*; D014316:Tritium
D013002:Somatomedins; D014316:Tritium; D005346:Fibroblast Growth Factors; D007334:Insulin-Like Growth Factor I; D015291:Transforming Growth Factors; D013936:Thymidine