CRF, urotensin I, and sauvagine stimulate the release of POMC-derived peptides from goldfish neurointermediate lobe cells.
In teleost fishes, the melanotropes of the neurointermediate lobe of the pituitary gland release numerous peptides--adrenocorticotropin (ACTH), melanotropin (MSH), lipotropin (LPH), corticotropin-like intermediate lobe peptide (CLIP), and endorphin--which are derived from the precursor molecule proopiomelanocortin. Superfused, isolated, dispersed goldfish neurointermediate lobe cell columns were used to investigate the release of immunoreactive (ir) alpha-MSH and ir ACTH from goldfish melanotropes. Stimulation of neurointermediate lobe cell columns with pulses of the structurally homologous peptides, Catostomus urotensin I (UI), ovine corticotropin-releasing factor (oCRF), or sauvagine, produced a significant increase in the concomitant release of ir alpha-MSH and ir ACTH. UI was two to three times as potent as ovine CRF or sauvagine. These studies suggest that CRF- and UI-like peptides stimulate the secretory activity of teleost melanotropes.
Tran
T N
TN
Department of Anatomy, Faculty of Medicine, University of Ottawa, Ontario, Canada.
Fryer
J N
JN
Lederis
K
K
Vaudry
H
H
D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D029845:Amphibian Proteins; D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000502:physiology*; D003530:Cyprinidae / Q000502:physiology*; D004723:Endorphins / Q000378:metabolism; D006054:Goldfish / Q000502:physiology*; D007030:Hypothalamo-Hypophyseal System / Q000502:physiology; D066298:In Vitro Techniques; D009074:Melanocyte-Stimulating Hormones / Q000378:metabolism; D036361:Peptide Hormones; D010455:Peptides / Q000502:physiology*; D010902:Pituitary Gland / Q000378:metabolism*; D011333:Pro-Opiomelanocortin / Q000378:metabolism*; D014579:Urotensins / Q000502:physiology*; D008083:beta-Lipotropin / Q000378:metabolism
D029845:Amphibian Proteins; D004723:Endorphins; D036361:Peptide Hormones; D010455:Peptides; D014579:Urotensins; D011333:Pro-Opiomelanocortin; C025727:sauvagine; D000324:Adrenocorticotropic Hormone; D009074:Melanocyte-Stimulating Hormones; D003346:Corticotropin-Releasing Hormone; D008083:beta-Lipotropin