Affinity chromatography of human plasma gelsolin with polyphosphate compounds on immobilized Cibacron Blue F3GA.
Human plasma gelsolin was specifically eluted from a Cibacron Blue F3GA column with 1 mM adenosine, guanosine, cytidine and uridine di- and triphosphates, except for cytidine 5'-diphosphate. Inorganic polyphosphates also eluted gelsolin, but neither nicotinamide adenine dinucleotide nor nicotinamide adenine dinucleotide phosphate did so. The results suggest that a terminal pyrophosphate structure might be essential for the specific elution. After elution with 1 mM adenosine 5'-triphosphate, additional gelsolin was eluted by washing the column with a high salt concentration. This indicates that human plasma gelsolin may bind to Cibacron Blue F3GA in at least two different ways.
Ito
H
H
Third Department of Internal Medicine, Osaka University School of Medicine, Japan.
Yamamoto
H
H
Kimura
Y
Y
Kambe
H
H
Okochi
T
T
Kishimoto
S
S
D000227:Adenine Nucleotides; D000255:Adenosine Triphosphate; D002135:Calcium-Binding Proteins / Q000097:blood*; D002846:Chromatography, Affinity; D003597:Cytosine Nucleotides; D004563:Electrochemistry; D004591:Electrophoresis, Polyacrylamide Gel; D018260:Gelsolin; D006801:Humans; D008840:Microfilament Proteins / Q000097:blood*; D011122:Polyphosphates; D014227:Triazines
D000227:Adenine Nucleotides; D002135:Calcium-Binding Proteins; D003597:Cytosine Nucleotides; D018260:Gelsolin; D008840:Microfilament Proteins; D011122:Polyphosphates; D014227:Triazines; C012842:Cibacron Blue F 3GA; D000255:Adenosine Triphosphate