[The production of anti-HBc IgM antibodies by human lymphocytes transformed by the Epstein-Barr virus].
In transformation by Epstein-Barr virus of lymphocytes derived from patients with acute hepatitis B, continuous cell cultures were obtained which produced anti-HBc IgM antibodies. These cell lines underwent from 50 to 150 passages. The level of the specific immunoglobulin production was shown to have a trend to decline; however, after cloning the antibody production became more stable. The antibodies produced by the cloned lymphocyte culture could not neutralize the antigen (unlike polyclonal antibodies) which indicated a high efficacy of cloning. When the solid phase was sensitized with produced immunoglobulins, specific activity of binding of HBc antigen detectable by anti-HBc conjugate with horseradish peroxidase was demonstrated. The study of sedimentation properties of antibodies produced by transformed lymphocytes showed their sedimentation constant to be 19S.
Gurov
A V
AV
Favorov
M O
MO
Iashina
T L
TL
Gol'dberg
E Z
EZ
Khizhniakova
T M
TM
Anan'ev
V A
VA
D002472:Cell Transformation, Viral / Q000276:immunology*; D002999:Clone Cells / Q000276:immunology; D006510:Hepatitis B Antibodies / Q000032:analysis / Q000096:biosynthesis*; D006512:Hepatitis B Core Antigens / Q000276:immunology*; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis / Q000096:biosynthesis*; D008214:Lymphocytes / Q000276:immunology*
D006510:Hepatitis B Antibodies; D006512:Hepatitis B Core Antigens; D007075:Immunoglobulin M
Produktsiia anti-HBc IgM-antitel limfotsitami cheloveka, transformirovannymi virusom Epshteĭna--Barr.