Herpes simplex virus type-1 immediate-early gene expression and shut off of host protein synthesis are inhibited in neomycin-treated human epidermoid carcinoma 2 cells.
Infection of human epidermoid carcinoma-2 (HEp-2) cells by Herpes simplex virus type 1 (HSV-1) leads to significant activation of inositol phospholipid turnover after 15 min. The effect of neomycin, an inhibitor of inositol phospholipid turnover, has been investigated for its effect on HSV-1 multiplication in HEp-2 cells. HSV-1 multiplication is inhibited by neomycin. This inhibition is not due to a block of virus adsorption or penetration. Neomycin inhibits the expression of virus immediate-early genes, as well as expression of early genes and viral DNA synthesis. In neomycin-treated cells, the usual virion-associated shut off of host protein synthesis does not occur. These results indicate that the inositol phospholipid pathway is involved in immediate-early gene expression and shut off of host protein synthesis in HEp-2 cells.
Garcin
D
D
Ecole Normale Supérieure de Lyon, Institut National de la Santé et de la Recherche Médicale, France.
Massé
T
T
Madjar
J J
JJ
Jacquemont
B
B
D000818:Animals; D015152:Blotting, Northern; D002294:Carcinoma, Squamous Cell; D004279:DNA, Viral / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D009355:Neomycin / Q000494:pharmacology*; D010716:Phosphatidylinositols / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D018139:Simplexvirus / Q000235:genetics* / Q000254:growth & development; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014709:Vero Cells; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics*; D014779:Virus Replication / Q000187:drug effects*
D004279:DNA, Viral; D010716:Phosphatidylinositols; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D009355:Neomycin; D013755:Tetradecanoylphorbol Acetate