title: Inhibitions of the GABA-induced currents of rat neurons by the alkaloid isocoryne from the plant Corydalis pseudoadunca.
abstract: A novel substance, isocoryne, affecting the GABA receptor complex, was isolated from the Corydalis pseudoadunca plant. The inhibitory effect of isocoryne on GABA-activated transmembrane current of single neurons isolated from trigeminal ganglia of rats was tested using concentration-clamp, voltage-clamp and intracellular perfusion techniques. Isocoryne, like other phthalide isoquinoline alkaloids with a 1S,9R isomeric form, produced an inhibitory effect on GABA-activated currents with a Ki = 10(-6) M.
authors.affiliation: Department of Physico-Chemical Biology of Cellular Membranes, A.A. Bogomoletz Institute of Physiology Academy of Sciences, Kiev, U.S.S.R.
authors.forename: N I
authors.initials: NI
authors.lastname: Chernevskaja
authors.forename: O A
authors.initials: OA
authors.lastname: Krishtal
authors.forename: A Y
authors.initials: AY
authors.lastname: Valeyev
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D044182:Benzylisoquinolines; D018756:GABA Antagonists; D066298:In Vitro Techniques; D009474:Neurons / Q000187:drug effects*; D010944:Plants / Q000032:analysis; D051381:Rats; D011963:Receptors, GABA-A / Q000187:drug effects; D013237:Stereoisomerism; D013329:Structure-Activity Relationship
chemical_list: D000470:Alkaloids; D044182:Benzylisoquinolines; D018756:GABA Antagonists; D011963:Receptors, GABA-A; C013024:hydrastine