Inhibition of the cooperative adhesion of Streptococcus sanguis to hydroxylapatite.
The adhesion of Streptococcus sanguis to hydroxylapatite is a process involving several adhesins and receptors. Binding isotherms and Scatchard plots of the adhesion suggest that cooperative interactions occur at low cell densities. It was found that sulfolane, a hydrophobic-bond diluent, was capable of inhibiting the cooperative adhesion of S. sanguis to saliva-coated hydroxylapatite beads. Sodium thiocyanate, a chaotropic agent, inhibited not only cooperative adhesion, but also the adhesion thought to result from noncooperative interactions. It is suggested that strong chaotropic agents may not only inhibit adhesin-receptor complexes, but also may influence the secondary/tertiary structures of interacting species.
Zhang
X H
XH
Academic Health Center, University of Louisville, KY 40292.
Rosenberg
M
M
Doyle
R J
RJ
D001422:Bacterial Adhesion / Q000187:drug effects*; D003292:Convulsants / Q000494:pharmacology; D017886:Durapatite; D006882:Hydroxyapatites; D013298:Streptococcus sanguis / Q000502:physiology*; D013861:Thiocyanates / Q000494:pharmacology; D013876:Thiophenes / Q000494:pharmacology
D003292:Convulsants; D006882:Hydroxyapatites; D013861:Thiocyanates; D013876:Thiophenes; D017886:Durapatite; C031760:thiocyanate; C013693:sulfolane