title: A complement-dependent neutralizing monoclonal antibody against glycoprotein II of pseudorabies virus.
abstract: A monoclonal antibody (MAb), 1.21, was produced against pseudorabies virus (PRV). It exhibited virus neutralization activity only in the presence of complement. Immunoblot analysis after sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of virions revealed that MAb 1.21 bound with the 230 kilodalton (kD) virus protein only under non-reducing conditions. This protein was purified by immuno-affinity chromatography using MAb 1.21 and was found to be composed of three subunits, 60, 70 and 120 kD polypeptides when analyzed by SDS-PAGE under reducing conditions. This protein is probably glycoprotein II of PRV.
authors.affiliation: Nippon Institute for Biological Science, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Ihara
authors.forename: T
authors.initials: T
authors.lastname: Nagata
authors.forename: A
authors.initials: A
authors.lastname: Ishihama
authors.forename: S
authors.initials: S
authors.lastname: Ueda
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D002460:Cell Line; D002846:Chromatography, Affinity; D003165:Complement System Proteins / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D006825:Hybridomas; D015151:Immunoblotting; D009500:Neutralization Tests; D014759:Viral Envelope Proteins / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000911:Antibodies, Monoclonal; D014759:Viral Envelope Proteins; C042966:pseudorabies virus glycoproteins; D003165:Complement System Proteins