Chromosomal assignment of the gene for the ubiquinone-binding protein of human mitochondrial cytochrome bc1 complex.
The ubiquinone-binding protein (QP-C) is a nuclear-encoded subunit of the cytochrome bc1 complex in the mitochondrial respiratory chain and is thought to be involved in the electron transfer reaction coupled to energy transduction. We recently isolated a nuclear gene for human QP-C and used, in the present study, its fragment as a probe for Southern blot analysis of EcoRI-digested DNAs prepared from 14 human-mouse somatic cell hybrids. The results indicated that the human QP-C gene is located on chromosome 8.
Hosokawa
Y
Y
Department of Biomedical Chemistry, Faculty of Medicine, University of Nagoya, Japan.
Suzuki
H
H
Nishikimi
M
M
Matsukage
A
A
Yoshida
M C
MC
Ozawa
T
T
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D002467:Cell Nucleus / Q000032:analysis; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015342:DNA Probes; D015246:Deoxyribonuclease EcoRI; D014450:Electron Transport Complex III / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells / Q000032:analysis; D051379:Mice; D009693:Nucleic Acid Hybridization
D002352:Carrier Proteins; D015342:DNA Probes; C027208:ubiquinone-binding proteins; D004247:DNA; D015246:Deoxyribonuclease EcoRI; D014450:Electron Transport Complex III