Orthopoxvirus gene expression in Xenopus laevis oocytes: a component of the virion is needed for late gene expression.
We have examined the feasibility of using Xenopus laevis oocytes microinjected with rabbit poxvirus as a system to study poxvirus gene expression. The injection of either intact virus or subviral cores resulted in accurate synthesis of viral proteins. This expression was dependent on the multiplicity of injected virus, with the optimal injected dose being equivalent to approximately 300 PFU per oocyte. Extensive viral gene expression including late viral protein synthesis was observed when intact virions were microinjected into the oocyte. However, the injection of subviral cores resulted in only early protein synthesis. When oocytes were injected with a mixture of subviral cores and the nonionic detergent-soluble fraction was removed from virus during the preparation of cores, both early and late viral proteins were synthesized. Therefore, the detergent-soluble fraction appears to contain a factor(s) required for the transition from early to late gene expression.
Massung
R F
RF
Department of Immunology and Medical Microbiology, College of Medicine, J. Hills Miller Health Center, University of Florida, Gainesville 32610.
Moyer
R W
RW
D000818:Animals; D000906:Antibodies; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D005814:Genes, Viral; D005821:Genetic Techniques; D008845:Microinjections; D008854:Microscopy, Electron; D009865:Oocytes / Q000378:metabolism* / Q000648:ultrastructure; D011212:Poxviridae / Q000235:genetics*; D014616:Vaccinia virus / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics*; D014771:Virion / Q000235:genetics*; D014982:Xenopus laevis
D000906:Antibodies; D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases