title: Effect of salvianolic acid A, a depside from roots of Salvia miltiorrhiza, on gastric H+,K(+)-ATPase.
abstract: Salvianolic acid A, a depside from the roots of Salvia miltiorrhiza, inhibited pig gastric H+,K(+)-ATPase and pNPPase with 50% inhibition values (IC50) of 5.2 x 10(-7) M and 1.7 x 10(-6) M, respectively. Kinetic studies revealed that the inhibition patterns induced by salvianolic acid A were competitive with respect to ATP and noncompetitive with respect to K+. Salvianolic acid A (25 mg/kg, i.p.) significantly inhibited acid secretion in pylorus-ligated rats. At the same dose it also showed a significant reduction in the formation of gastric lesion induced by water immersion and restraint stress. These results suggest that salvianolic acid A shows antisecretory and antiulcer activity by inhibiting the gastric H+,K(+)-ATPase.
authors.affiliation: Research Center, Taisho Pharmaceutical Co. Ltd., Ohmiya, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Murakami
authors.forename: H
authors.initials: H
authors.lastname: Kijima
authors.forename: Y
authors.initials: Y
authors.lastname: Isobe
authors.forename: M
authors.initials: M
authors.lastname: Muramatsu
authors.forename: H
authors.initials: H
authors.lastname: Aihara
authors.forename: S
authors.initials: S
authors.lastname: Otomo
authors.forename: L N
authors.initials: LN
authors.lastname: Li
authors.forename: C B
authors.initials: CB
authors.lastname: Ai
mesh_terms: D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors*; D000818:Animals; D000897:Anti-Ulcer Agents / Q000302:isolation & purification / Q000494:pharmacology; D002109:Caffeic Acids / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D053630:Depsides; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D005744:Gastric Acid / Q000378:metabolism; D005753:Gastric Mucosa / Q000201:enzymology*; D017506:H(+)-K(+)-Exchanging ATPase; D062385:Hydroxybenzoates / Q000302:isolation & purification / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D007773:Lactates / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D015394:Molecular Structure; D051381:Rats; D011919:Rats, Inbred Strains; D013276:Stomach Ulcer / Q000188:drug therapy; D013552:Swine
chemical_list: D000897:Anti-Ulcer Agents; D002109:Caffeic Acids; D053630:Depsides; D004365:Drugs, Chinese Herbal; D062385:Hydroxybenzoates; D007773:Lactates; C066201:salvianolic acid A; D000251:Adenosine Triphosphatases; D017506:H(+)-K(+)-Exchanging ATPase