title: Activity of human volunteer sera to candidate Plasmodium falciparum circumsporozoite protein vaccines in the inhibition of sporozoite invasion assay of human hepatoma cells and hepatocytes.
abstract: Sera from human volunteers immunized with either synthetic peptide (NANP)3-TT or recombinant protein R32tet32 Plasmodium falciparum CS vaccines were tested in the inhibition of sporozoite invasion (ISI) assays using human hepatoma (HepG2-A16) cells or primary human hepatocytes. Sera or purified immunoglobulin (Ig) from volunteers who were completely protected against P. falciparum sporozoite challenge had higher ISI activity than sera from non-protected volunteers, or the highest titre endemic serum. However, Ig from protected and non-protected volunteers did not block sporozoite invasion of human hepatocytes, suggesting that P. falciparum sporozoites invade hepatocytes by mechanisms which differ from those concerned with invasion of HepG2-A16 cells.
authors.affiliation: Biomedical Research Institute, Rockville, MD 20852.
authors.forename: M R
authors.initials: MR
authors.lastname: Hollingdale
authors.forename: A
authors.initials: A
authors.lastname: Appiah
authors.forename: P
authors.initials: P
authors.lastname: Leland
authors.forename: V E
authors.initials: VE
authors.lastname: do Rosario
authors.forename: D
authors.initials: D
authors.lastname: Mazier
authors.forename: S
authors.initials: S
authors.lastname: Pied
authors.forename: D A
authors.initials: DA
authors.lastname: Herrington
authors.forename: J D
authors.initials: JD
authors.lastname: Chulay
authors.forename: W R
authors.initials: WR
authors.lastname: Ballou
authors.forename: T
authors.initials: T
authors.lastname: Derks
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D006528:Carcinoma, Hepatocellular / Q000276:immunology; D002478:Cells, Cultured; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology; D008099:Liver / Q000276:immunology; D008288:Malaria / Q000517:prevention & control*; D009842:Oligopeptides / Q000276:immunology*; D010963:Plasmodium falciparum / Q000276:immunology*; D015800:Protozoan Proteins / Q000276:immunology*; D016052:Protozoan Vaccines / Q000276:immunology*; D014407:Tumor Cells, Cultured / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D007136:Immunoglobulins; D009842:Oligopeptides; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; C052953:(asparaginyl-alanyl-asparaginyl-proline)3