title: [Macromolecular spin pH-probes on the basis of human serum albumin].
abstract: Human serum albumin has been chemically modified by two different spin pH-sensitive labels of the imidazoline series containing in their structure alkylating and carboxyl groups, respectively. The ESR spectra of spin-labeled proteins are sensitive to pH of the medium. The pK values of spin-labeled proteins measured by the ESR method are: pKI = 3.2 +/- 0.1; pKII = 4.75 +/- 0.1. The resulting macromolecular spin pH probes may be used for measuring the local values of pH by the ESR technique within the pH range of 1.8-6.2.
authors.forename: V V
authors.initials: VV
authors.lastname: Khramtsov
authors.forename: L M
authors.initials: LM
authors.lastname: Vaĭner
authors.forename: V A
authors.initials: VA
authors.lastname: Rar
authors.forename: T A
authors.initials: TA
authors.lastname: Berezina
authors.forename: V V
authors.initials: VV
authors.lastname: Martin
authors.forename: L B
authors.initials: LB
authors.lastname: Volodarskiĭ
mesh_terms: D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012709:Serum Albumin / Q000737:chemistry*; D013113:Spin Labels
chemical_list: D012709:Serum Albumin; D013113:Spin Labels
vernacular_title: Makromolekuliarnye spinovye pH-zondy na osnove syvorotochnogo al'bumina cheloveka.