[The use of mini-Mu-replicons for cloning of various structural genes of Yersinia pestis].
The possibility to clone the structural leu gene of Yersinia pestis in vivo using the mini-Mu bacteriophages with the inserted plasmid replicones has been demonstrated. The E. coli K12 transductants having obtained the Leu(+)-marker within the cloned 4.8-21 kb fragments stably inherited the leu-gene within the autonomous mini-Mu replicones. The possibility to clone other Yersinia pestis genes by the same technique has been demonstrated.
Rakin
A V
AV
Rykova
V A
VA
D010583:Bacteriophage mu / Q000235:genetics*; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005822:Genetic Vectors; D012093:Replicon; D014169:Transformation, Bacterial; D015010:Yersinia pestis / Q000235:genetics*
D004251:DNA Transposable Elements
Ispol'zovanie mino-Mu-replikonov dlia klonirovaniia nekotorykh strukturnykh genov chumnogo mikroba.