Insertion of the fusion gene from Newcastle disease virus into a non-essential region in the terminal repeats of fowlpox virus and demonstration of protective immunity induced by the recombinant.
In this paper we report on the identification of non-essential genes in the terminal repeats of the avipox-virus fowlpox virus and the use of these as insertion sites in a vector system. Foreign genes inserted into these sites are shown to be present in two copies in the resultant recombinant virus. To test the potential use of this vector as a live vaccine the fusion gene of Newcastle disease virus has been inserted into a vaccine strain of fowlpox virus and inoculated into chickens. The experiments demonstrate the ability of the recombinant to protect chickens against challenge by a virulent strain of Newcastle disease virus and to elicit the formation of an anti-fusion protein antibody.
Boursnell
M E
ME
Institute for Animal Health, Houghton Laboratory, Huntingdon, Cambridgeshire, U.K.
Green
P F
PF
Campbell
J I
JI
Deuter
A
A
Peters
R W
RW
Tomley
F M
FM
Samson
A C
AC
Chambers
P
P
Emmerson
P T
PT
Binns
M M
MM
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002645:Chickens; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004797:Enzyme-Linked Immunosorbent Assay; D005587:Fowlpox virus / Q000235:genetics* / Q000276:immunology; D005814:Genes, Viral; D005822:Genetic Vectors; D006385:Hemagglutination Inhibition Tests; D007109:Immunity; D009522:Newcastle disease virus / Q000235:genetics* / Q000276:immunology; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
D004251:DNA Transposable Elements