beta-Endorphin-like and adrenocorticotropin-like hormones in the pituitary of the snake Ptyas mucosa.
Snake (Ptyas mucosa) pituitaries were heated, extracted with an acidic medium, and the extract subsequently chromatographed on carboxymethyl cellulose (CMC). Fractions were assayed for their abilities to displace D-ala2-D-leu5-[tyrosyl, 3,53H] enkephalin from binding to rat brain membranes and 125I-beta-endorphin from binding to its antibody, and to stimulate corticosterone production by isolated rat adrenal cells. The fraction unadsorbed on CMC (having the least basic character) had the lowest opiate receptor binding activity and beta-endorphin-like immunoreactivity. The highest steroidogenic activity, opiate receptor binding activity and beta-endorphin-like immunoreactivity were concentrated in a strongly adsorbed fraction. Snake pituitaries were also extracted with Tris-HCl buffer (pH 7.8). The extract from the second procedure was subsequently chromatographed on ConA-Sepharose, ultrafiltered and dialyzed to obtain a nonglycopeptide fraction with a molecular weight between 1,000 and 10,000. This fraction also exhibited steroidogenic and opiate receptor binding activities.
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Department of Biochemistry, Faculty of Medicine, Chinese University Hong Kong, Shatin, New Territories.
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D000324:Adrenocorticotropic Hormone / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D002852:Chromatography, Ion Exchange; D003348:Cortisone / Q000096:biosynthesis; D008297:Male; D010902:Pituitary Gland / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D011957:Receptors, Opioid / Q000378:metabolism; D012911:Snakes / Q000378:metabolism*; D001615:beta-Endorphin / Q000032:analysis* / Q000378:metabolism
D011957:Receptors, Opioid; D001615:beta-Endorphin; D000324:Adrenocorticotropic Hormone; D003348:Cortisone