title: Insulin-like growth factors and the developing and mature rat small intestine: receptors and biological actions.
abstract: To determine if insulin-like growth factor-I (IGF-I) or multiplication stimulating activity (MSA, rat IGF-II) might directly influence small intestinal epithelium, we studied the distribution of IGF binding sites during development of the rat intestine. Cell membranes from suckling rat mucosa bound 2-6 times as much 125I-IGF-I and 3-5 times as much 125I-MSA as did adult membranes. Isolated villus cells from suckling and adult rats specifically bound both IGFs. IGF-I binding tended to remain high during suckling, whereas MSA binding fell progressively from the early suckling period. Competitive displacement studies with insulin, IGF-I and MSA demonstrated the presence of type-I and type-II IGF receptors. In vitro autoradiography of 125I-IGF-I binding sites in adult and suckling rat jejunum showed highest binding in the submucosa with extensions up into the lamina propria. Immunocytochemical localization of type-II receptors showed highest density in villus epithelium and vessel walls. Administration of MSA by oral and IGF-I by oral and parenteral routes (1 microgram/day for 6 days) to suckling rats stimulated jejunal brush border enzymes, but not intestinal growth. Developmental changes in receptor density and effects on brush border enzymes suggest a specific role for IGFs in post-natal development of the rat intestine.
authors.affiliation: University of Melbourne, Department of Medicine, Royal Melbourne Hospital, Vic., Australia.
authors.forename: G P
authors.initials: GP
authors.lastname: Young
authors.forename: T M
authors.initials: TM
authors.lastname: Taranto
authors.forename: H A
authors.initials: HA
authors.lastname: Jonas
authors.forename: A J
authors.initials: AJ
authors.lastname: Cox
authors.forename: A
authors.initials: A
authors.lastname: Hogg
authors.forename: G A
authors.initials: GA
authors.lastname: Werther
mesh_terms: D000818:Animals; D000833:Animals, Suckling; D001345:Autoradiography; D007124:Immunoenzyme Techniques; D007334:Insulin-Like Growth Factor I / Q000502:physiology*; D007335:Insulin-Like Growth Factor II / Q000502:physiology*; D007413:Intestinal Mucosa / Q000737:chemistry; D007421:Intestine, Small / Q000737:chemistry / Q000254:growth & development*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000502:physiology*; D017451:Receptors, Somatomedin; D013002:Somatomedins / Q000502:physiology
chemical_list: D011956:Receptors, Cell Surface; D017451:Receptors, Somatomedin; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II