Expression of adenovirus type 2 DNA polymerase in insect cells infected with a recombinant baculovirus.
Sequences encoding adenovirus type 2 DNA polymerase were placed under control of the polyhedrin promoter and inserted into the baculovirus Autographa californica nuclear polyhedrosis virus by homologous recombination. Insect cells infected with the recombinant virus produced substantial amounts of the adenovirus type 2 DNA polymerase protein which was functional in both DNA polymerase and replication initiation reactions. Thus, the baculovirus expression system can provide active adenovirus type 2 DNA polymerase that is produced in quantities suitable for biochemical and structural analysis.
Watson
C J
CJ
Department of Biochemistry and Microbiology, University of St Andrews, Fife, UK.
Hay
R T
RT
D000260:Adenoviruses, Human / Q000201:enzymology / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002458:Cell Fractionation; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004257:DNA Polymerase II / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D004261:DNA Replication; D004274:DNA, Recombinant / Q000235:genetics / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics* / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D007304:Insect Viruses / Q000235:genetics*; D008969:Molecular Sequence Data; D009036:Moths; D011401:Promoter Regions, Genetic
D004274:DNA, Recombinant; D004279:DNA, Viral; D004257:DNA Polymerase II