Autoradiographic localization of receptor sites of 3H-muscimol in the CNS of rats.
Radiolabelled muscimol (3-hydroxy-5-aminomethylisoxazole) a potent gamma-amino-butyric acid (GABA) agonist was continuously perfused throughout intraventriculocisternal system of the rat brain. Then the brain tissue was prepared for autoradiography. GABA receptor sites as indicated by 3H-muscimol binding and autoradiographic silver grains display different patterns of distribution over numerous cerebral and spinal cord structures. The precise cellular localization of 3H-muscimol in rat neostriatum was described and compared with other labelled regions of rat brain. The new subpopulations of local neostriatal medium size neurons labelled by 3H-muscimol was identified. The morphology and distribution of the specific neuron subpopulation is discussed. Autoradiographic approach using synthetic analogues structurally related to GABA represent a new tool for morphological demonstration of GABA receptor sites.
Parízek
J
J
D000818:Animals; D001345:Autoradiography; D002490:Central Nervous System / Q000378:metabolism*; D008297:Male; D009118:Muscimol / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011963:Receptors, GABA-A / Q000032:analysis* / Q000378:metabolism
D011963:Receptors, GABA-A; D009118:Muscimol