Frequent expression of receptors for granulocyte-macrophage colony-stimulating factor on human nonhematopoietic tumor cell lines.
Receptors for granulocyte-macrophage colony-stimulating factor (GM-CSF) were identified on 9 of 35 (26%) human nonhematopoietic tumor cell lines including non-small cell lung cancer, stomach cancer, colon cancer, and osteosarcoma cells. GM-CSF receptors distributed on these human tumor cells were low affinity types with an equilibrium dissociation constant of 1.5-10.0 nM. Cross-linking studies revealed that the molecular weights of the low affinity GM-CSF receptors were 65-85 kilodaltons. The high affinity receptors identified on hematopoietic cells were not detected on human nonhematopoietic tumor cells which we studied, and we could detect no effects of GM-CSF on cell growth of these tumor cells.
Miyagawa
K
K
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Chiba
S
S
Shibuya
K
K
Piao
Y F
YF
Matsuki
S
S
Yokota
J
J
Terada
M
M
Miyazono
K
K
Takaku
F
F
D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure*; D002471:Cell Transformation, Neoplastic / Q000378:metabolism / Q000473:pathology; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure*; D015870:Gene Expression / Q000502:physiology; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure*; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D016184:Receptors, Colony-Stimulating Factor; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure*; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure
D011956:Receptors, Cell Surface; D016184:Receptors, Colony-Stimulating Factor