Monoclonal antibodies to endothelin: application for sandwich immunoassays.
We established 16 monoclonal antibodies (MAbs) to endothelin (ET), a novel and potent vasoconstrictor. They were classified into two groups: eight of them reacted with the C-terminal portion of ET, and the other eight reacted with the non-C-terminal portion. We constructed the sandwich enzyme linked immunosorbent assays (ELISAs) using a combination of a MAb belonging to the former with that belonging to the latter, and could detect about 400 pg/ml of ET.
Hamaoki
M
M
Diagnostic Division, Yamasa Shoyu Co., Ltd., Chiba, Japan.
Kato
H
H
Sugi
M
M
Fujimoto
M
M
Kurihara
H
H
Yosizumi
M
M
Yanagisawa
M
M
Kimura
S
S
Masaki
T
T
Yazaki
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal; D016232:Endothelins; D004730:Endothelium, Vascular / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000276:immunology; D010455:Peptides / Q000032:analysis / Q000276:immunology*; D014662:Vasoconstrictor Agents / Q000032:analysis / Q000276:immunology
D000911:Antibodies, Monoclonal; D016232:Endothelins; D010455:Peptides; D014662:Vasoconstrictor Agents