Multi-center clinical evaluation of a chairside method for detecting certain periodontopathic bacteria in periodontal disease.
The association of bacteroides gingivalis, Bacteroides forsythus, Treponema denticola, and Actinobacillus actinomycetemcomitans among others with periodontal disease offers the opportunity for the development of diagnostic tests that are based upon the detection and/or quantification of one or more of these organisms or their by-products in the plaque. Three of the putative periodontal pathogens namely, T. denticola, B. gingivalis, and B. forsythus, can hydrolyze the synthetic trypsin substrate, N-benzoyl-DL-arginine-2-naphthylamide (BANA) forming a color reaction. The present investigation evaluated a commercially developed solid state assay for BANA hydrolysis that can be read after 15 minutes incubation at chairside. A total of 702 subgingival plaque samples were collected from 117 patients seen at four university dental clinics and placed on reagent cards. The color development on the cards was compared to the presence of T. denticola and B. gingivalis in the plaque, and with the clinical appearance of the sampled sites. This multi-center study demonstrated that antibodies to B. gingivalis and T. denticola could detect these organisms by an ELISA in the majority of the subgingival plaque samples. Comparable information could be obtained when the same plaques were evaluated by the reagent card format for BANA hydrolysis. The ELISA and reagent card were comparable in their ability to distinguish between clinically healthy and diseased sites. Both diagnostic procedures detected the periodontopathogens in plaques from sites that were judged clinically healthy.(ABSTRACT TRUNCATED AT 250 WORDS)
Loesche
W J
WJ
University of Michigan School of Dentistry, Ann Arbor.
Bretz
W A
WA
Lopatin
D
D
Stoll
J
J
Rau
C F
CF
Hillenburg
K L
KL
Killoy
W J
WJ
Drisko
C L
CL
Williams
R
R
Weber
H P
HP
D000328:Adult; D001120:Arginine / Q000031:analogs & derivatives*; D001439:Bacteroides / Q000302:isolation & purification; D001587:Benzoylarginine-2-Naphthylamide; D003773:Dental Plaque / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005188:False Negative Reactions; D005189:False Positive Reactions; D005260:Female; D005884:Gingival Hemorrhage / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D015337:Multicenter Studies as Topic; D010510:Periodontal Diseases / Q000175:diagnosis / Q000382:microbiology*; D011934:Reagent Strips; D014208:Treponema / Q000302:isolation & purification
D011934:Reagent Strips; D001587:Benzoylarginine-2-Naphthylamide; D001120:Arginine