Is ubiquitin involved in the dedifferentiation of higher plant cells?
Transformation of a mesophyll cell into a viable protoplast that is able to re-enter the cell cycle, divide and further differentiate into organs, is accompanied by a very rapid and important increase in ubiquitin gene expression. Three major size classes of ubiquitin mRNA transcripts were observed in protoplasts as soon as they were isolated and incubated in their culture medium. The 1.6 kb mRNAs were expressed in response to the stress caused by the isolation procedure. They decreased after a few hours of incubation in the culture medium. The 1.9 kb and the 1.3 kb mRNAs enhanced in protoplasts were also observed in young leaves and in actively dividing cells: they appeared to be developmental-stage specific. They are not expressed in response to the stress, but may be considered to be part of the dedifferentiation program induced in protoplasts. These results suggest, as the enhancement of ubiquitin gene expression coincides with the dramatic changes in gene expression observed in protoplasts, that ubiquitin may play a fundamental role in the process of cellular dedifferentiation.
Jamet
E
E
Institut de Biologie Moléculaire des Plantes, Strasbourg, France.
Durr
A
A
Parmentier
Y
Y
Criqui
M C
MC
Fleck
J
J
D015152:Blotting, Northern; D015139:Blotting, Southern; D002454:Cell Differentiation / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D005786:Gene Expression Regulation; D010947:Plants, Toxic; D012333:RNA, Messenger / Q000096:biosynthesis; D014026:Nicotiana / Q000166:cytology* / Q000235:genetics; D014452:Ubiquitins / Q000235:genetics*
D012333:RNA, Messenger; D014452:Ubiquitins