title: Soybean lipoxygenase-1 is not a quinoprotein.
abstract: Soybean lipoxygenase-1 was reinvestigated with respect to its quinoprotein nature. It has been reported previously that soybean lipoxygenase-1 contains pyrroloquinoline quinone as the organic cofactor. Because spectroscopic data were found to be inconsistent with the evidence presented in [1], we sought to reproduce the published data by carefully following the procedures described in [1] and supplementing them with new analytical results. The combined data lead us to conclude that soybean lipoxygenase-1 is not a quinoprotein.
authors.affiliation: Bijvoet Center for Biomolecular Research, Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.
authors.forename: G A
authors.initials: GA
authors.lastname: Veldink
authors.forename: H
authors.initials: H
authors.lastname: Boelens
authors.forename: M
authors.initials: M
authors.lastname: Maccarrone
authors.forename: F
authors.initials: F
authors.lastname: van der Lecq
authors.forename: J F
authors.initials: JF
authors.lastname: Vliegenthart
authors.forename: M A
authors.initials: MA
authors.lastname: Paz
authors.forename: R
authors.initials: R
authors.lastname: Fluckiger
authors.forename: P M
authors.initials: PM
authors.lastname: Gallop
mesh_terms: D003067:Coenzymes / Q000032:analysis; D005562:Formazans / Q000032:analysis; D000441:Hexanols; D008084:Lipoxygenase / Q000737:chemistry*; D045542:PQQ Cofactor; D010659:Phenylhydrazines; D015363:Quinolones / Q000032:analysis*; D013025:Glycine max / Q000201:enzymology*; D013053:Spectrophotometry
chemical_list: D003067:Coenzymes; D005562:Formazans; D000441:Hexanols; D010659:Phenylhydrazines; D015363:Quinolones; C030299:phenylhydrazine; C004787:2,4-dinitrophenylhydrazine; D045542:PQQ Cofactor; D008084:Lipoxygenase