Chemical composition, particle size range, and biological activity of some low molecular weight heparin derivatives.
Several low molecular weight (LMW) heparin sodium derivatives from different sources, as well as some related regular heparin sodium preparations, were examined for chemical composition by high field (300 MHz) 1H NMR spectroscopy, for particle size range by quasi-elastic light scattering (QELS) methods, and for anti-coagulation potency and anti-factor Xa activity by the standard U.S. Pharmacopeial assays described for regular heparin. The NMR spectra provided insight into possible modes of depolymerization used to generate the LMW heparins, as well as into the presence of dermatan sulfate or other chemical contaminants. The QELS analysis permitted the heparin preparations to be characterized and compared by virtue of their distinctive particle size distributions.
Neville
G A
GA
Bureau of Drug Research, Health and Welfare Canada, Ottawa, ON.
Mori
F
F
Racey
T J
TJ
Rochon
P
P
Holme
K R
KR
Perlin
A S
AS
D003871:Dermatan Sulfate / Q000032:analysis; D006495:Heparin, Low-Molecular-Weight / Q000032:analysis* / Q000494:pharmacology / Q000528:radiation effects; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D010316:Particle Size; D012542:Scattering, Radiation
D006495:Heparin, Low-Molecular-Weight; D003871:Dermatan Sulfate