Ethanol oxidation by deermice mitochondria under physiologic conditions.
Mitochondria obtained from alcohol dehydrogenase-positive or - negative deermice do not oxidize significant amounts of ethanol at pH 7.4. A slight activity, equivalent to less than 0.3% of the elimination rate in alcohol dehydrogenase-negative deermice was observed at pH 10; it was strongly inhibited by cyanide and thiourea, and was not dependent on exogenous NAD. Whereas ethanol oxidation by the cytosol of alcohol dehydrogenase-positive deermice was time-dependent, that of mitochondria from alcohol dehydrogenase-negative deermice was not. These findings indicate that deermice mitochondria do not oxidize ethanol at physiological pH, and that the mitochondrial system is not likely to play a significant physiologic role.
Inatomi
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N
Alcohol Research and Treatment Center, VA Medical Center, Bronx, New York 10468.
Ito
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D
Lieber
C S
CS
D000426:Alcohol Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D003600:Cytosol / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005838:Genotype; D066298:In Vitro Techniques; D007700:Kinetics; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism; D008930:Mitochondria, Liver / Q000201:enzymology / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D010542:Peromyscus
D009243:NAD; D000431:Ethanol; D000426:Alcohol Dehydrogenase