title: Effect of inhibitors on conformational changes in hen lysozyme around thermal transition point in solution and solid state.
abstract: Carbon-13 NMR spectroscopy has been used to further document the interaction, at low and high temperatures, of N-acetylglucosamine and its short polymers with hen egg-white lysozyme. The results have been compared with the corresponding X-ray crystallographic data. Two domains, the active site and the hydrophobic box, have been found by NMR to undergo conformational rearrangement while X-ray crystallography only detected changes located in the active site. The extent of the modifications induced by inhibitor binding was proportional to the inhibitor size. The two techniques concurred to show that even in the presence of monosaccharide (N-acetylglucosamine), more than one subsite of the enzyme was occupied at high temperature, the binding at the C-site being the best defined. The thermal transition of lysozyme still occurred in solution when inhibitors were bound. However, in the solid state, crystallographic data showed that the transition was hindered.
authors.affiliation: Centre for Biological and Medical Magnetic Resonance Studies, UA CNRS No. 1186, Faculty of Medicine, University of Aix-Marseilles, Marseilles, France.
authors.forename: M
authors.initials: M
authors.lastname: Bernard
authors.forename: P
authors.initials: P
authors.lastname: Canioni
authors.forename: P
authors.initials: P
authors.lastname: Cozzone
authors.forename: J
authors.initials: J
authors.lastname: Berthou
authors.forename: P
authors.initials: P
authors.lastname: Jollès
mesh_terms: D000117:Acetylglucosamine / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D050260:Carbohydrate Metabolism; D002247:Carbon Isotopes; D002645:Chickens; D003460:Crystallization; D005260:Female; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D009113:Muramidase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D011522:Protons; D012996:Solutions; D013816:Thermodynamics; D014961:X-Ray Diffraction
chemical_list: D002247:Carbon Isotopes; D011522:Protons; D012996:Solutions; D009113:Muramidase; D000117:Acetylglucosamine