Synthesis and processing of a gp28/32 membrane glycoprotein induced by Marek's disease virus serotype 2.
The post-translational events leading from the precursor to the processed forms of a glycoprotein with an Mr of 28K to 32K (gp27/32) of Marek's disease virus (MDV) serotype 2 were examined with pulse-chase experiments and treatment with tunicamycin and monensin. Cell-free translation of infected cell mRNA followed by immunoprecipitation analysis suggested that a polypeptide with a size of 22K is the initial precursor. Experiments with endo-beta-N-acetylglucosaminidase H and endo-beta-N-acetylglucosaminidase F indicated that gp28/32 contains mostly N-linked oligosaccharides of the complex type. These studies showed that 22K, the initial product, is then processed through intermediates to the 28K to 32K form.
Nakajima
K
K
Department of Pathology, Osaka University, Japan.
Shibayama
T
T
Kato
S
S
Kurimura
T
T
Hirai
K
K
D004591:Electrophoresis, Polyacrylamide Gel; D008381:Herpesvirus 2, Gallid / Q000145:classification / Q000378:metabolism*; D011233:Precipitin Tests; D011499:Protein Processing, Post-Translational; D012703:Serotyping; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000378:metabolism*
D014759:Viral Envelope Proteins