title: Control of the spatial distribution of sodium channels in giant fiber lobe neurons of the squid.
abstract: Na+ channels are present at high density in squid giant axon but are absent from its somata in the giant fiber lobe (GFL) of the stellate ganglion. GFL cells dispersed in vitro maintain growing axons and develop a Na+ channel distribution similar to that in vivo. Tunicamycin, a glycosylation inhibitor, selectively disrupts the spatially appropriate, high level expression of Na+ channels in axonal membrane but has no effect on expression in cell bodies, which show low level, inappropriate expression in vitro. This effect does not appear to involve alteration in Na+ channel turnover or axon viability. K+ channel distribution is unaffected. Thus, glycosylation appears to be involved in controlling Na+ channel localization in squid neurons.
authors.affiliation: Hopkins Marine Station of Stanford University, Department of Biological Sciences, Pacific Grove, California 93950.
authors.forename: W F
authors.initials: WF
authors.lastname: Gilly
authors.forename: M T
authors.initials: MT
authors.lastname: Lucero
authors.forename: F T
authors.initials: FT
authors.lastname: Horrigan
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D002478:Cells, Cultured; D049832:Decapodiformes / Q000378:metabolism*; D005728:Ganglia, Sympathetic / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D015222:Sodium Channels / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors; D014018:Tissue Distribution; D014415:Tunicamycin / Q000494:pharmacology
chemical_list: D015222:Sodium Channels; D014415:Tunicamycin