A colorimetric assay for the determination of acid nucleoside triphosphatase activity.
A photometric method for the determination of the acid nucleoside triphosphatase (EC 3.6.1.-) is described, in which inorganic phosphate is liberated from ATP or other nucleoside triphosphates. Colorimetric determination of liberated phosphate is based on the formation of a green complex of phosphomolybdate and malachite green hydrochloride. Optimal test conditions were evaluated as well as the sample preparation. The enzyme activities measured in 100 normal human sera are in the range of 0.5 to 9.0 U/l with an average of 4.0 U/l for men and 3.8 U/l for women.
Kirchgesser
M
M
Institut für Klinische Biochemie, Universität Bonn.
Dahlmann
N
N
D003124:Colorimetry; D004396:Coloring Agents; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D008982:Molybdenum; D043583:Nucleoside-Triphosphatase; D010744:Phosphoric Monoester Hydrolases / Q000097:blood* / Q000302:isolation & purification; D012016:Reference Values; D012394:Rosaniline Dyes
D004396:Coloring Agents; D007202:Indicators and Reagents; D012394:Rosaniline Dyes; C005095:malachite green; D008982:Molybdenum; D010744:Phosphoric Monoester Hydrolases; D043583:Nucleoside-Triphosphatase; C022175:ammonium molybdate