In vitro transcription and translation of the human 1,25-dihydroxyvitamin D3 receptor cDNA.
A fragment of the complementary deoxyribonucleic acid to the human 1,25-dihydroxyvitamin D3 receptor protein containing essentially the entire open reading frame was transcribed and translated in vitro. The resulting protein was then demonstrated to exhibit the physical and functional features, i.e. molecular weight, immunoreactivity, 1,25-dihydroxyvitamin D3 binding, and DNA-cellulose binding, of the native human receptor from the T47D cell line. This validates the authenticity of the cDNA in a cell free system and provides a biochemical means of generating this rare and labile macromolecule to use in heretofore difficult structure/function studies.
Terpening
C M
CM
Department of Biochemistry, University of Arizona, Tucson 85724.
Haussler
M R
MR
D002117:Calcitriol / Q000378:metabolism; D002460:Cell Line; D002474:Cell-Free System / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis*; D006801:Humans; D066298:In Vitro Techniques; D008970:Molecular Weight; D016366:Open Reading Frames; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid / Q000096:biosynthesis / Q000235:genetics*; D013329:Structure-Activity Relationship; D014158:Transcription, Genetic
D018167:Receptors, Calcitriol; D011987:Receptors, Steroid; D004247:DNA; D002117:Calcitriol