UV cross-linking of distinct proteins to the PRDII domain of the interferon-beta promoter.
The human interferon-beta gene (IFN-beta) is transcriptionally activated in a variety of cell types, mediated in part by a decameric element, designated the PRDII domain (-55 to -64) which is the target for the transcription factor NF-kappa B. In the present study, we have examined factor binding to the PRDII domain in three cell lines (HeLa, U937, Jurkat) following treatment with known inducers of IFN-beta transcription and NF-kappa B binding activity. UV cross-linking analysis has revealed that in all 3 cell types two inducible proteins of 58 kD and 69 kD and one constitutive protein of 48 kD bind to the PRDII domain. These experiments suggest that multiple proteins may interact with this element to regulate IFN-beta gene transcription.
Xanthoudakis
S
S
Lady Davis Institute for Medical Research, Sir Mortimer B. Davis Jewish General Hospital, Montreal, Quebec, Canada.
Hiscott
J
J
D001483:Base Sequence; D002460:Cell Line; D003432:Cross-Linking Reagents; D003513:Cycloheximide / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000528:radiation effects; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D007370:Interferon Type I / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D016328:NF-kappa B; D015345:Oligonucleotide Probes; D010222:Parainfluenza Virus 1, Human / Q000235:genetics; D011070:Poly I-C / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000528:radiation effects*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014157:Transcription Factors / Q000378:metabolism* / Q000528:radiation effects; D014158:Transcription, Genetic; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D007370:Interferon Type I; D016328:NF-kappa B; D015345:Oligonucleotide Probes; D014157:Transcription Factors; D003513:Cycloheximide; D013755:Tetradecanoylphorbol Acetate; D011070:Poly I-C