[In vitro and in vivo effect of thyroid hormones on the growth of neuroblastoma cells. II. The effect of thyroxine in vivo].
We studied the effect of thyroxine (T4 0.050 mg/kg/d, i.p.), TSH (0.08 U/kg/d, i.p.) and hypothalamic peptide (HF; 1 mg protein/kg/d, i.p.) given alone or in combination, on the growth of murine (NB C-1300) and human (NB Park) neuroblastoma transplanted onto the nude mouse (nu/nu). Both T4 and TSH caused a significant increase (perchlorate a decrease) of the serum T3. Histologically, the T4 treatment was followed by partial tumor necrosis and a marked growth of connective tissue within the tumors; there was no significant change in tumor weight as compared to the control group. Treatment with HF alone or in combination with T4 inhibited in 30% the invasive growth of the neuroblastoma transplants and a fatty degeneration was found in 25% of the human NB-TX after 28 days of treatment. The measurement of the intratumoral content of the cyclic nucleotides showed a significant increase of the cAMP and a decrease of the cGMP. The morphological and biochemical alteration observed under treatment with thyroid hormone or analogues could possibly be applied for therapeutic purposes.
Busse
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Abteilung für Hämatologie und Onkologie, Johann Wolfgang Goethe-Universität, Frankfurt/Main, BRD.
Baum
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Hör
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Kornhuber
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D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D004359:Drug Therapy, Combination; D013007:Growth Hormone-Releasing Hormone / Q000627:therapeutic use; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009447:Neuroblastoma / Q000188:drug therapy* / Q000378:metabolism; D009955:Ornithine Decarboxylase / Q000378:metabolism; D013972:Thyrotropin / Q000627:therapeutic use; D013974:Thyroxine / Q000627:therapeutic use*; D014183:Transplantation, Heterologous
D013972:Thyrotropin; D013007:Growth Hormone-Releasing Hormone; D000242:Cyclic AMP; D009955:Ornithine Decarboxylase; D006152:Cyclic GMP; D013974:Thyroxine
In-vitro- und in-vivo-Wirkung von Schilddrüsenhormon auf das Wachstum von Neuroblastomzellen. II. Effeckt von Thyroxin in vivo.