[Separation of Thomsen-Friedenreich (T) antigen and N antigen precursor glycoproteins from perchloric acid-soluble fraction of cyst fluid of human ovarian clear cell carcinoma and their some chemical and serological properties].
One M perchloric acid-soluble fraction separated from cyst fluid of a patient (blood group AB) with ovarian clear cell carcinoma in malignant was subjected to Sephacryl S-200 gel filtration and Fraction 1 which was eluted at void volume, reacted with both of the lectins of Vicia unijuga and Arachis hypogaea. This fraction was then separated into 6 fractions (DEAE-Frs. 1-6) in a DEAE-Sephacel column chromatography. DEAE-Frs. 3-6 were sugar-rich glycoprotein fractions which contained galactose and N-acetylglucosamine in remarkably high percentages, respectively. Reactivities against various hemagglutinins of these four fractions suggest that DEAE-Fr. 3 is a Thomsen-Friedenreich (T) glycoprotein fraction with AB activity and weak N antigen precursor activity and DEAE-Fr. 4 is an N antigen precursor glycoprotein fraction with AB activity and weak T activity.
Yanagi
K
K
Department of Biochemistry, Tokyo College of Pharmacy, Japan.
Ohyama
K
K
Yamakawa
T
T
Watanabe
H
H
Hirakawa
S
S
Ohkuma
S
S
D000230:Adenocarcinoma / Q000032:analysis*; D015295:Antigens, Tumor-Associated, Carbohydrate; D002845:Chromatography; D004187:Disaccharides / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D005260:Female; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000032:analysis*; D010472:Perchlorates; D011498:Protein Precursors / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D012995:Solubility
D015295:Antigens, Tumor-Associated, Carbohydrate; D004187:Disaccharides; D006023:Glycoproteins; D010472:Perchlorates; D011498:Protein Precursors; C016467:Thomsen-Friedenreich antigen