title: Lysosomal trafficking in rat cardiac myocytes.
abstract: By immunolabeling of cryosections, we have characterized in rat cardiac myocytes the cation-independent mannose-6-phosphate receptor (MPR), a lysosomal membrane glycoprotein, lgp120, and a lysosomal enzyme, MEP (homologous to cathepsin L). Most of the MPR label was located in large membrane-filled structures (MPR structures) in large clusters of mitochondria adjacent to but distinct from the Golgi complex. Lpg120 and MEP showed typical lysosomal localization throughout the cell, often associated with regions that appeared to contain autophagosome-like structures. In addition, MEP and lgp120 co-localized within MPR structures. MEP and MPR were localized inside the lumen of MPR structures. MPR was associated mostly with inner membranes, whereas lgp120 was predominantly bound to the outer limiting membrane. MPR, lgp120, and MEP were not detected in Golgi stacks, but some labeling was seen in the putative TGN. Our data suggest that the MPR structures are prelysosomes involved in lysosomal enzyme targeting in rat cardiac myocytes.
authors.affiliation: Department of Cell Biology, University of Jyväskylä, Finland.
authors.forename: V S
authors.initials: VS
authors.lastname: Marjomäki
authors.forename: A P
authors.initials: AP
authors.lastname: Huovila
authors.forename: M A
authors.initials: MA
authors.lastname: Surkka
authors.forename: I
authors.initials: I
authors.lastname: Jokinen
authors.forename: A
authors.initials: A
authors.lastname: Salminen
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015703:Antigens, CD; D056668:Cathepsin L; D002403:Cathepsins / Q000032:analysis; D002417:Cattle; D003546:Cysteine Endopeptidases; D010450:Endopeptidases; D005455:Fluorescent Antibody Technique; D005629:Frozen Sections; D015151:Immunoblotting; D007425:Intracellular Membranes / Q000032:analysis; D008099:Liver / Q000032:analysis; D052118:Lysosomal-Associated Membrane Protein 1; D051907:Lysosomal Membrane Proteins; D008247:Lysosomes / Q000032:analysis / Q000648:ultrastructure*; D008562:Membrane Glycoproteins / Q000032:analysis; D008854:Microscopy, Electron; D008929:Mitochondria, Heart / Q000648:ultrastructure; D009206:Myocardium / Q000648:ultrastructure*; D051381:Rats; D017527:Receptor, IGF Type 2; D011956:Receptors, Cell Surface / Q000032:analysis
chemical_list: D015703:Antigens, CD; D052118:Lysosomal-Associated Membrane Protein 1; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins; D017527:Receptor, IGF Type 2; D011956:Receptors, Cell Surface; D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D056668:Cathepsin L; C534280:Ctsl protein, rat