Enzyme-linked immunosorbent assay for the detection of porcine epidemic diarrhea coronavirus antibodies in swine sera.
An enzyme-linked immunosorbent assay (ELISA) for detecting serum antibodies to the porcine epidemic diarrhea coronavirus (PEDV) was established by using cell culture-grown PEDV as antigen for coating. Ultracentrifugation through 20 and 45% (w/w) sucrose cushions proved to be the best antigen purification method. Examination of 1024 swine sera showed a high specificity and a greater sensitivity of the ELISA, when compared with indirect immunofluorescence. Reference sera with high antibody titers to PEDV originated from two pigs experimentally infected with PEDV. Three different antigen purification methods and the advantages of the ELISA compared with an immunofluorescence test are discussed.
Hofmann
M
M
Institute of Virology, University of Zurich, Switzerland.
Wyler
R
R
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology / Q000302:isolation & purification; D002499:Centrifugation, Density Gradient; D003332:Coronaviridae / Q000276:immunology* / Q000648:ultrastructure; D003333:Coronaviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D003967:Diarrhea / Q000175:diagnosis / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D008854:Microscopy, Electron; D011237:Predictive Value of Tests; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*; D014461:Ultracentrifugation
D000914:Antibodies, Viral; D000956:Antigens, Viral