[(NA++K+)-ATPase inhibitor from bovine hypothalamus].
The presence of an endogenous ouabain-like substance in bovine hypothalamus is demonstrated on the bas is of the ability of acid acetone extracts of hypothalamus to inhibit (NA+ + K+)-ATPase activity, 86Rb uptake, 22Na efflux and (3H)ouabain binding. Partial purification of the substance was achieved by acetic acid fractionation followed by sephadex-G25 chromatography and subsequent desalting by mixed-bed resin. The results are discussed with relation to the important role of the sodium pump inhibitor in the regulation of (N+ + K+)-ATPase activity and sodium transport and hence in the pathophysiologic mechanisms of essential hypertension.
Fu
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Y
D000818:Animals; D002417:Cattle; D006801:Humans; D007031:Hypothalamus / Q000737:chemistry*; D008297:Male; D008638:Mesenteric Arteries; D009131:Muscle, Smooth, Vascular / Q000201:enzymology; D011506:Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors; D011817:Rabbits; D051381:Rats; D011921:Rats, Inbred WKY; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000097795:ATPase Inhibitory Protein
D011506:Proteins; D006180:Proton-Translocating ATPases; D000254:Sodium-Potassium-Exchanging ATPase; D000097795:ATPase Inhibitory Protein