title: An improved method for isolating cardiac myocytes useful for patch-clamp studies.
abstract: An improved method for isolating cardiac myocytes is described. This method consists of the initial dispersion of the myocytes with a new type of collagenase and the following treatment of the cells with a non-specific protease. This method gives more than 80% rod-shaped, viable, and quiescent cells, which are suitable for biochemical as well as electrophysiological experiments. Formation of a G omega seal and subsequent whole-cell recording is easily performed using these cells.
authors.affiliation: National Institute for Physiological Sciences, Okazaki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yazawa
authors.forename: M
authors.initials: M
authors.lastname: Kaibara
authors.forename: M
authors.initials: M
authors.lastname: Ohara
authors.forename: M
authors.initials: M
authors.lastname: Kameyama
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D004594:Electrophysiology; D005260:Female; D006168:Guinea Pigs; D006321:Heart / Q000502:physiology; D066298:In Vitro Techniques; D003012:Microbial Collagenase; D009206:Myocardium / Q000166:cytology*; D010447:Peptide Hydrolases; D011188:Potassium / Q000378:metabolism
chemical_list: D010447:Peptide Hydrolases; D003012:Microbial Collagenase; D011188:Potassium