title: The ammonium sulfate activation of phosphorylase b.
abstract: The ammonium sulfate activation of phosphorylase b has been studied. Ammonium sulfate, when present in high concentrations, induces properties of phosphorylase a in phosphorylase b, such as an enhanced affinity for AMP, a reversal of the glucose-6-P inhibition and enzyme tetramerization. The data are consistent with the interpretation that sulfates bind to the Ser-14 site and the sulfate-protein interactions at this site are responsible for activation of phosphorylase b.
authors.affiliation: Biological Research Center, National Hellenic Research Foundation, Athens, Greece.
authors.forename: D D
authors.initials: DD
authors.lastname: Leonidas
authors.forename: N G
authors.initials: NG
authors.lastname: Oikonomakos
authors.forename: A C
authors.initials: AC
authors.lastname: Papageorgiou
authors.forename: A
authors.initials: A
authors.lastname: Xenakis
authors.forename: C T
authors.initials: CT
authors.lastname: Cazianis
authors.forename: F
authors.initials: F
authors.lastname: Bem
mesh_terms: D000249:Adenosine Monophosphate / Q000494:pharmacology; D000645:Ammonium Sulfate / Q000494:pharmacology*; D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D004789:Enzyme Activation / Q000187:drug effects; D005958:Glucosephosphates / Q000378:metabolism; D007700:Kinetics; D046911:Macromolecular Substances; D009132:Muscles / Q000201:enzymology*; D010763:Phosphorylase b / Q000378:metabolism*; D006005:Phosphorylases / Q000378:metabolism*; D011817:Rabbits; D014461:Ultracentrifugation
chemical_list: D005958:Glucosephosphates; D046911:Macromolecular Substances; D000249:Adenosine Monophosphate; C031590:glucose-1-phosphate; D010763:Phosphorylase b; D006005:Phosphorylases; D000645:Ammonium Sulfate