title: Failure to demonstrate a major role for Kupffer cells and radiosensitive leukocytes in immunoglobulin-mediated elimination of Trypanosoma musculi.
abstract: Previous studies have indicated that elimination of parasitemia in Trypanosoma musculi infection is brought about by immunoglobulin G2a antibodies, C3, and an effector cell. Experiments were designed to identify the putative effector cell by using several approaches. Infected C5-deficient or C5-sufficient mice treated with silica particles or given 900 rads of radiation 3 days earlier effectively eliminated trypanosomes following administration of immune plasma (IP). Silica-treated, noninfected mice given T. musculi preincubated with IP also cleared the parasites. Radiolabeling studies revealed that uptake of the cleared trypanosomes by the liver in normal mice was relatively low (24%) and fell only slightly (19%) in silica-treated mice. In contrast, uptake of radiolabeled sheep erythrocytes by the liver was normally much higher (47%) and fell drastically (7%) in silica-treated mice. Mice were then immunocompromised by 900 rads of radiation, silica particles, and anti-platelet serum combined before IP-sensitized trypanosomes were given. Leukocyte and platelet counts were both reduced by 95% and sheep erythrocyte uptake by the liver fell from 77 to 5%; however, greater than 99% of the injected trypanosomes were cleared in these mice and uptake of radiolabeled trypanosomes by the liver was similar to that of normal mice. Lastly, in anesthetized mice in which Kupffer cells were excluded surgically from the circulation, greater than 99% of the IP-sensitized trypanosomes disappeared rapidly from the blood. Only 7% of the radiolabel was found in the liver versus 60% in sham-operated mice. The results are interpreted as showing that hepatic Kupffer cells play a minor role in the immune elimination of T. musculi. Likewise, radiosensitive leukocytes and platelets are unlikely to be sole candidates for the putative effector cell that mediates a cure of murine trypanosomiasis.
authors.affiliation: Montreal General Hospital Research Institute, Quebec, Canada.
authors.forename: P A
authors.initials: PA
authors.lastname: Kongshavn
authors.forename: K
authors.initials: K
authors.lastname: Shaw
authors.forename: E
authors.initials: E
authors.lastname: Ghadirian
authors.forename: O
authors.initials: O
authors.lastname: Ulczak
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D001792:Blood Platelets / Q000502:physiology; D004546:Elapid Venoms / Q000494:pharmacology; D007109:Immunity / Q000528:radiation effects; D007116:Immunization, Passive; D007668:Kidney / Q000469:parasitology; D007728:Kupffer Cells / Q000276:immunology*; D007958:Leukocyte Count; D007962:Leukocytes / Q000276:immunology*; D008099:Liver / Q000276:immunology / Q000469:parasitology; D008168:Lung / Q000469:parasitology; D010976:Platelet Count; D012822:Silicon Dioxide; D013154:Spleen / Q000469:parasitology; D014345:Trypanosoma / Q000276:immunology; D014352:Trypanosomiasis / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D004546:Elapid Venoms; C016310:cobra venom factor; D012822:Silicon Dioxide