Primary structure of the S peplomer gene of bovine coronavirus and surface expression in insect cells.
The nucleotide sequence of the S peplomer gene of bovine coronavirus (BCV) has been determined. A single open reading frame of 4089 nucleotides encodes a polypeptide of 150K with 20 potential sites for addition of N-linked oligosaccharides. Expression of the cloned BCV S gene by a recombinant of Autographa californica nuclear polyhedrosis virus resulted in production of a 180K glycosylated polypeptide which was transported to the surface of the cell. Comparison of the BCV S gene with the analogous genes of murine hepatitis viruses shows that the BCV S polypeptide contains a unique domain of 138 amino acids not present in murine hepatitis virus strain JHM, but which has a partially homologous counterpart in strain A59. This domain accounts for most of the differences in size of the S gene products of these coronaviruses.
Parker
M D
MD
Veterinary Infectious Disease Organization, Saskatoon, Saskatchewan, Canada.
Yoo
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Cox
G J
GJ
Babiuk
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LA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D003332:Coronaviridae / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D015967:Gene Expression Regulation, Viral; D006023:Glycoproteins / Q000235:genetics*; D007304:Insect Viruses / Q000235:genetics; D008969:Molecular Sequence Data; D009036:Moths; D006517:Murine hepatitis virus / Q000235:genetics; D011233:Precipitin Tests; D012367:RNA, Viral / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics*
D006023:Glycoproteins; D012367:RNA, Viral; D014759:Viral Envelope Proteins