Complementation of the rev gene mutation among human and simian lentiviruses.
The functional exchangeability of the rev gene was assessed in transient transfection experiments by using in vitro-constructed rev and gag mutants of the following three primate lentiviruses: human immunodeficiency virus type 1 (HIV-1), HIV-2, and simian immunodeficiency virus from the African green monkey (SIV AGM). Cotransfection into SW480 cells of the rev and gag mutants derived from the DNA of each infectious virus resulted in the generation of progeny particles as determined by reverse transcriptase assay. rev gene mutants of HIV-2 and SIV AGM were also complemented by all gag mutants derived from the three viruses. In contrast, no evidence of complementation was obtained following cotransfection of the HIV-1 rev mutant and the gag mutant of HIV-2 or SIV AGM.
Sakai
H
H
Department of Viral Oncology, Kyoto University, Japan.
Shibata
R
R
Miura
T
T
Hayami
M
M
Ogawa
K
K
Kiyomasu
T
T
Ishimoto
A
A
Adachi
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D002872:Chromosome Deletion; D003110:Colonic Neoplasms; D004279:DNA, Viral / Q000235:genetics; D005091:Exons; D015694:Gene Products, rev / Q000235:genetics; D005814:Genes, Viral; D015757:Genes, rev; D005816:Genetic Complementation Test; D015497:HIV-1 / Q000235:genetics*; D015498:HIV-2 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D015302:Simian Immunodeficiency Virus / Q000235:genetics*; D014162:Transfection; D054321:rev Gene Products, Human Immunodeficiency Virus
D004279:DNA, Viral; D015694:Gene Products, rev; D054321:rev Gene Products, Human Immunodeficiency Virus