Specific expression of human c-fgr in natural immunity effector cells.
The c-fgr gene product was shown by immune complex protein kinase assay with specific antibodies to be a 58-kilodalton protein (p58c-fgr) with tyrosine-specific autophosphorylating activity. On examination of peripheral blood cells by immunoblotting with anti-c-fgr antibodies, p58c-fgr was found only in the fractions of monocytes, granulocytes, and natural killer cells. On the other hand, histochemical studies of hybridization demonstrated accumulation of c-fgr transcripts on most monocytes and large lymphocytes. In hematopoietic cell lines, p58c-fgr was detected in differentiated granulocytic cells as well as in differentiated monocytic cells of HL-60-cell origin. These data suggest a specific role for p58c-fgr in natural immunity effector cells.
Inoue
K
K
Department of Oncology, University of Tokyo, Japan.
Yamamoto
T
T
Toyoshima
K
K
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex / Q000032:analysis; D002478:Cells, Cultured; D006801:Humans; D007113:Immunity, Innate; D007694:Killer Cells, Natural / Q000201:enzymology* / Q000276:immunology; D007962:Leukocytes / Q000201:enzymology; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology; D019061:src-Family Kinases
D000906:Antibodies; D000936:Antigen-Antibody Complex; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D011505:Protein-Tyrosine Kinases; C053102:proto-oncogene proteins c-fgr; D019061:src-Family Kinases