Automatic computation of enzyme kinetics by HPLC.
Enzyme activity can be easily measured by HPLC using traces of the product itself as an internal standard. Our procedure involved the development of an equation using the experimental data obtained in the kinetic assay. The entire procedure can thus be automated and a computer program is presented here for facilitating the assay and saving time. The determination of the activity of NAD kinase is reported as an example.
Mauri
P L
PL
Dipartimento di Scienze e Tecnologie Biomediche, University of Milano, Italy.
Bitonti
M
M
Pace
M
M
Pietta
P G
PG
Agnellini
D
D
D000465:Algorithms; D002851:Chromatography, High Pressure Liquid; D004798:Enzymes / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008432:Mathematical Computing; D010770:Phosphotransferases / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor); D011381:Programming Languages; D012985:Software Design
D004798:Enzymes; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C021844:NAD kinase