Effects of subacute toluene exposure on neuronal and glial marker proteins in rat brain.
The behavior of marker proteins of neurons (gamma-enolase) and glial cells (alpha-enolase, beta-S100 protein and creatine kinase-B) was investigated quantitatively by using enzyme immunoassay systems in toluene-exposed rat brains. Three groups of animals were exposed to toluene vapor at 300 ppm, 1000 ppm, and 3000 ppm, respectively, 8 h/day, 6 days/week, for 2 weeks. After subacute repeated solvent exposure, both neuron-specific gamma-enolase and glial marker proteins displayed an overall concentration-dependent increase tendency in separate brain regions. In cerebrum, only the 3000 ppm group showed a significant increase in alpha-enolase by 27% and creatine kinase-B (CK-B) by 26%. alpha-Enolase and gamma-enolase exhibited a pronounced elevation in cerebellum relative to other brain regions, while beta-S100 protein appeared to be the most markedly altered marker in brainstem. The development of gliosis, which is a frequent phenomenon following CNS damage, is presumed to be responsible for the elevation of glial marker content. Energy metabolism disruption in brain tissues may also bring about the compensatory oversynthesis of glycolytic enzymes such as gamma-enolase, alpha-enolase and CK-B. The dose-dependent alteration patterns following toluene exposure suggest the feasibility of using these brain specific markers to evaluate solvent-induced CNS effects.
Huang
J
J
Department of Hygiene, Nagoya University School of Medicine, Japan.
Kato
K
K
Shibata
E
E
Hisanaga
N
N
Ono
Y
Y
Takeuchi
Y
Y
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D003402:Creatine Kinase / Q000378:metabolism; D007124:Immunoenzyme Techniques; D007527:Isoenzymes; D008297:Male; D009419:Nerve Tissue Proteins / Q000187:drug effects*; D009457:Neuroglia / Q000187:drug effects* / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D009418:S100 Proteins / Q000187:drug effects; D014050:Toluene / Q000633:toxicity*
D015415:Biomarkers; D007527:Isoenzymes; D009419:Nerve Tissue Proteins; D009418:S100 Proteins; D014050:Toluene; D003402:Creatine Kinase; D010751:Phosphopyruvate Hydratase