A structural protein encoded by the 5' region of the hepatitis C virus genome efficiently detects viral infection.
A structural region of the hepatitis C virus genome was molecularly cloned. A protein expressed in vitro by transcription followed by translation was precipitated immunologically by sera from patients with chronic hepatitis or hepatocellular carcinoma that were positive for antibody against the non-structural protein, C100, of hepatitis C virus, but not by sera from healthy persons. Thus, this structural protein should be useful for detection of infection with this virus.
Kato
N
N
Virology Division, National Cancer Center Research Institute, Tokyo.
Hijikata
M
M
Ootsuyama
Y
Y
Nakagawa
M
M
Ohkoshi
S
S
Shimotohno
K
K
D000595:Amino Acid Sequence; D000956:Antigens, Viral; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis; D003001:Cloning, Molecular; D005814:Genes, Viral; D016174:Hepacivirus / Q000235:genetics*; D006526:Hepatitis C / Q000175:diagnosis*; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011994:Recombinant Proteins / Q000235:genetics; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics*
D000956:Antigens, Viral; C064544:C100 protein, hepatitis C virus; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins