Production of infectious swine vesicular disease virus from cloned cDNA in mammalian cells.
Full-length cDNA clones of the swine vesicular disease virus (SVDV) were constructed from subgenomic cDNA clones in the expression vector pSVL (pSVLS00). The direct transfection of mammalian cells with plasmid pSVLS00 results in the production of infectious virus. The recovered virus was neutralized completely by anti-SVDV guinea-pig serum, but did show a difference in plaque morphology from the parental virus.
Inoue
T
T
Exotic Disease Research Division, National Institute of Animal Health, Tokyo, Japan.
Yamaguchi
S
S
Saeki
T
T
Sekiguchi
K
K
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D004770:Enterovirus / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D007700:Kinetics; D009500:Neutralization Tests; D013552:Swine; D013555:Swine Vesicular Disease / Q000382:microbiology; D014162:Transfection; D010948:Viral Plaque Assay; D014779:Virus Replication
D004279:DNA, Viral