Regulation of foreign gene in fowlpox virus by a vaccinia virus promoter.
A vaccinia virus promoter was evaluated for regulation of a foreign gene in fowlpox virus by a transient expression assay. Fowlpox virus-infected quail cells, transfected with plasmid DNA containing chloramphenicol acetyltransferase (CAT) gene ligated to a vaccinia virus promoter, expressed CAT activity. No CAT activity was detected either in uninfected cells or fowlpox virus-infected cells. These results indicated that a heterologous vaccinia virus promoter can regulate expression of a foreign gene in fowlpox virus.
Tripathy
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Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana 61801.
Wittek
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D000818:Animals; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics; D003370:Coturnix; D004279:DNA, Viral / Q000235:genetics; D005587:Fowlpox virus / Q000235:genetics*; D015967:Gene Expression Regulation, Viral; D011401:Promoter Regions, Genetic; D014162:Transfection; D014616:Vaccinia virus / Q000235:genetics*
D004279:DNA, Viral; D015500:Chloramphenicol O-Acetyltransferase