Molecular cloning and chromosome mapping of the human gene encoding protein phosphotyrosyl phosphatase 1B.
The inactivation of growth suppressor genes appears to play a major role in the malignant process. To assess whether protein phosphotyrosyl phosphatases (protein-tyrosine-phosphate phosphohydrolase, EC 3.1.3.48) function as growth suppressors, we have isolated a cDNA clone encoding human protein phosphotyrosyl phosphatase 1B for structural and functional characterization. The translation product deduced from the 1305-nucleotide open reading frame predicts a protein containing 435 amino acids and having a molecular mass of 49,966 Da. The amino-terminal 321 amino acids deduced from the cDNA sequence are identical to the empirically determined sequence of protein phosphotyrosyl phosphatase 1B. [Charbonneau, H., Tonks, N. K., Kumar, S., Diltz, C. D., Harrylock, M., Cool, D. E., Krebs, E. G., Fischer, E. H. & Walsh, K. A. (1989) Proc. Natl. Acad. Sci. USA 86, 5252-5256]. A genomic clone has been isolated and used in an in situ hybridization to banded metaphase chromosomes to determine that the gene encoding protein phosphotyrosyl phosphatase 1B maps as a single-copy gene to the long arm of chromosome 20 in the region q13.1-q13.2.
Brown-Shimer
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Applied bioTechnology, Inc., Cambridge, MA 02142.
Johnson
K A
KA
Lawrence
J B
JB
Johnson
C
C
Bruskin
A
A
Green
N R
NR
Hill
D E
DE
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002890:Chromosomes, Human, Pair 20; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D014176:Protein Biosynthesis; D017027:Protein Tyrosine Phosphatases; D012689:Sequence Homology, Nucleic Acid
D015345:Oligonucleotide Probes; D004247:DNA; D010749:Phosphoprotein Phosphatases; D017027:Protein Tyrosine Phosphatases