title: 6DMAP inhibits chromatin decondensation but not sperm histone kinase in sea urchin male pronuclei.
abstract: Treatment of sea urchin eggs for 10 min prior to fertilization with the kinase inhibitor 6DMAP (6-dimethylaminopurine) reversibly inhibits swelling and loss of conical morphology of the male pronucleus. Male pronuclei inhibited with 1 mM 6DMAP for 25 min undergo phosphorylation of Sp H1 and Sp H2B histones as fully as do control nuclei. Therefore, Sp histone kinase, whose target sequences resemble those of the M-phase histone kinase, is not inhibited by 6DMAP, and Sp histone phosphorylation, although it may be necessary, is not sufficient for chromatin decondensation.
authors.affiliation: Department of Biology, Amherst College, Massachusetts 01002.
authors.forename: D
authors.initials: D
authors.lastname: Poccia
authors.forename: W
authors.initials: W
authors.lastname: Pavan
authors.forename: G R
authors.initials: GR
authors.lastname: Green
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects* / Q000648:ultrastructure; D002843:Chromatin / Q000187:drug effects* / Q000378:metabolism; D005306:Fertilization; D006657:Histones / Q000378:metabolism; D008297:Male; D010766:Phosphorylation; D006656:Protamine Kinase / Q000037:antagonists & inhibitors*; D047428:Protein Kinase Inhibitors; D012617:Sea Urchins; D015053:Zygote / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D002843:Chromatin; D006657:Histones; D047428:Protein Kinase Inhibitors; C001020:N(6),N(6)-dimethyladenine; D006656:Protamine Kinase; D000225:Adenine