title: Neuropeptide K is present in human cerebrospinal fluid.
abstract: Neurokinin A-like immunoreactivity (NKA-LI) in human cerebrospinal fluid (CSF) was determined by radioimmuno assay (RIA) combined with high performance liquid chromatography (HPLC). The major immunoreactive component did not coelute with NKA, but coeluted with neuropeptide K (NPK), which contains the NKA sequence in its C-terminus. Trypsin treatment of this component from human CSF and of synthetic NPK, produced a substance which coeluted with NKA in the HPLC system. When the NKA-LI was oxidized with hydrogen peroxide and rechromatographed, the immunoreactivity coeluted with NPK sulfoxide. The results indicate that the main part of the NKA-LI in CSF is identical with NPK. The mean concentration of NPK measured in CSF from 6 healthy subjects by HPLC-RIA was 23 +/- 11 (SD) pmol/L.
authors.affiliation: Department of Clinical Pharmacology, Karolinska Institute, Huddinge Hospital, Sweden.
authors.forename: G
authors.initials: G
authors.lastname: Toresson
authors.forename: C
authors.initials: C
authors.lastname: de las Carreras
authors.forename: E
authors.initials: E
authors.lastname: Brodin
authors.forename: L
authors.initials: L
authors.lastname: Bertilsson
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D015288:Neurokinin A / Q000134:cerebrospinal fluid; D015287:Neurokinin B / Q000134:cerebrospinal fluid; D009479:Neuropeptides / Q000134:cerebrospinal fluid*; D010084:Oxidation-Reduction; D011863:Radioimmunoassay; D015320:Tachykinins; D014357:Trypsin
chemical_list: D009479:Neuropeptides; D015320:Tachykinins; C045206:neuropeptide K; D015288:Neurokinin A; D015287:Neurokinin B; D014357:Trypsin