[A strategy for immortalizing lines committed to endoderm, neuroectoderm or mesoderm from mouse teratocarcinoma].
With the aim of immortalizing embryonic cells fixed at early embryonic stages, various plasmids carrying the SV40 early region were introduced into the mouse embryonal carcinomas (EC) F9 and 1003. Only the construction PK4, in which the SV40 oncogenes are placed under the control of the adenovirus E1A promoter, led to the immortalization of the cells at the onset of differentiation. Clones corresponding to committed precursors of each embryonic lineage (neuroectoderm, mesoderm and endoderm) were then selected with high efficiency according to the following strategy: selection of immature cells which: have lost EC cell markers, keep a stable phenotype, are immortalized by the expression of the SV40 oncogenes and are still able to differentiate along a restricted lineage in vitro or in vivo. Examples of an endodermal precursor (H7) which differentiates into extraembryonic and embryonic endoderm, of a neuroectodermic clone (ICII) committed to a serotoninergic differentiation, and of a mesodermal osteogenic clone (CI) which gives rise to bone in vivo and in vitro, are given.
Buc-Caron
M H
MH
Institut Pasteur, Paris, France.
Launay
J M
JM
Marie
P J
PJ
Kellermann
O
O
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D002454:Cell Differentiation; D002461:Cell Line, Transformed; D003001:Cloning, Molecular; D004475:Ectoderm / Q000473:pathology*; D004707:Endoderm / Q000473:pathology*; D008648:Mesoderm / Q000473:pathology*; D051379:Mice; D009857:Oncogenes; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000235:genetics; D013539:Simian virus 40 / Q000235:genetics; D013234:Stem Cells / Q000473:pathology; D013724:Teratoma / Q000473:pathology*; D014407:Tumor Cells, Cultured
Une stratégie pour immortaliser des lignées orientées vers l'endoderme, le neuroectoderme ou le mésoderme à partir du tératocarcinome de la souris.