title: Purification of an endothelin receptor from human placenta.
abstract: We have identified an endothelin (ET) binding protein on the membranes of human placenta and purified it to homogeneity. It is a polypeptide with an apparent Mol. Wt. of 40,000 and is a major protein to be labeled by cross-linking with either 125I-ET-1, -2, or -3. Binding studies with Scatchard analysis indicated the presence of a single class, high-affinity binding site with Kds of 57 pM, 480 pM and 40 nM for 125I-labeled ET-1, ET-2 and ET-3, respectively. These results suggest that the 40K protein is a major ET receptor in placenta and, most likely, can bind differentially to ET-1, ET-2 and ET-3.
authors.affiliation: Department of Molecular Genetics, Nippon Roche Research Center, Kamakura, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Wada
authors.forename: H
authors.initials: H
authors.lastname: Tabuchi
authors.forename: R
authors.initials: R
authors.lastname: Ohba
authors.forename: M
authors.initials: M
authors.lastname: Satoh
authors.forename: Y
authors.initials: Y
authors.lastname: Tachibana
authors.forename: N
authors.initials: N
authors.lastname: Akiyama
authors.forename: O
authors.initials: O
authors.lastname: Hiraoka
authors.forename: A
authors.initials: A
authors.lastname: Asakura
authors.forename: C
authors.initials: C
authors.lastname: Miyamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Furuichi
mesh_terms: D000345:Affinity Labels; D002846:Chromatography, Affinity; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D016232:Endothelins; D004730:Endothelium, Vascular / Q000378:metabolism*; D005260:Female; D006801:Humans; D010455:Peptides / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011956:Receptors, Cell Surface / Q000302:isolation & purification*; D017466:Receptors, Endothelin
chemical_list: D000345:Affinity Labels; D003432:Cross-Linking Reagents; D016232:Endothelins; D010455:Peptides; D011956:Receptors, Cell Surface; D017466:Receptors, Endothelin