title: Desensitization of Bacillus subtilis aspartokinase I to allosteric inhibition by meso-diaminopimelate allows aspartokinase I to function in amino acid biosynthesis during exponential growth.
abstract: Strains of Bacillus subtilis deficient in aspartokinases II and III are unable to grow in the absence of lysine, methionine, and threonine, although they have normal levels of aspartokinase I (J.J. Zhang, F.M. Hu, N.Y. Chen, and H. Paulus, J. Bacteriol. 172:701-708, 1990). Revertants with the ability to grow in the absence of lysine and methionine had an altered aspartokinase I, which was insensitive to feedback inhibition by meso-diaminopimelate. This suggests that inhibition by meso-diaminopimelate limits the ability of aspartokinase I to function in amino acid biosynthesis.
authors.affiliation: Department of Metabolic Regulation, Boston Biomedical Research Institute, Massachusetts 02114.
authors.forename: J J
authors.initials: JJ
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: Paulus
mesh_terms: D000494:Allosteric Regulation; D000599:Amino Acids, Diamino / Q000494:pharmacology*; D001222:Aspartate Kinase / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000302:isolation & purification; D001224:Aspartic Acid / Q000096:biosynthesis; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D003960:Diaminopimelic Acid / Q000494:pharmacology*; D007527:Isoenzymes / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000302:isolation & purification; D007700:Kinetics; D010770:Phosphotransferases / Q000037:antagonists & inhibitors*
chemical_list: D000599:Amino Acids, Diamino; D007527:Isoenzymes; D001224:Aspartic Acid; D003960:Diaminopimelic Acid; D010770:Phosphotransferases; D001222:Aspartate Kinase