Granule cell induction of 9-O-acetyl gangliosides on cerebellar glia in microcultures.
In previous studies we have shown that the expression of acetylated gangliosides recognized by the JONES monoclonal antibody is correlated with regions of cell migration in the developing rat nervous system. In this study we have investigated the expression of these gangliosides in two different types of cultures prepared from dissociated postnatal rat cerebella. In the first type, cells are plated after dissociation under conditions where most of the glial cells develop a stellate morphology that anchors neurons but does not support their migration. In the second type of culture, cells are plated in a ratio of four neurons to one glial cell and under these conditions the predominant form of astroglia is an elongate form that supports the migration of granule neurons. Granule neurons express JONES antigens in dissociated cell suspensions and in cultures in which cells are plated either after dissociation or in a 4:1 neuron:glia ratio. On the other hand, glial cells grown in the absence of neurons are JONES negative. In addition, the expression of JONES gangliosides by glial cells is different in the two types of culture. In cultures where the astroglial cells display the stellate morphology only a small proportion show JONES staining. Cultures in which the glial cells assume the elongate morphology have a significantly higher number of JONES-positive astroglia.
Mendez-Otero
R
R
Instituto de Biologia, Universidade do Estado do Rio de Janeiro, Brazil.
Constantine-Paton
M
M
D000107:Acetylation; D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D005732:Gangliosides / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D008858:Microscopy, Phase-Contrast; D009457:Neuroglia / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology; D051381:Rats; D014746:Vimentin / Q000032:analysis
D000911:Antibodies, Monoclonal; D005732:Gangliosides; D005904:Glial Fibrillary Acidic Protein; D014746:Vimentin