title: Glycosylation of the murine erythropoietin receptor.
abstract: Murine erythropoietin-responsive Rauscher Red 5-1.5 cells were used to determine the contribution of glycosylation to the size and function of the erythropoietin receptor. The half life of the receptors was determined to be 4 h. The number of receptors was not significantly decreased in cells treated for 48 h with inhibitors of glycosylation (tunicamycin, glucosamine or swainsonine) and their affinity was slightly enhanced in tunicamycin- or glucosamine-treated cells. Erythropoietin was cross-linked with two proteins of 104 and 86 kDa. Their molecular masses were not significantly reduced in cells treated with the glycosylation inhibitors. When immunoprecipitated cross-linked receptors were digested with endoglycosidases, the molecular masses of both proteins were only slightly modified giving values of 100 and 82 kDa. Thus we can conclude that the proteins cross-linked to erythropoietin are very weakly glycosylated.
authors.affiliation: ICGM, Inserm U152, Hôpital Cochin, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Mayeux
authors.forename: N
authors.initials: N
authors.lastname: Casadevall
authors.forename: O
authors.initials: O
authors.lastname: Muller
authors.forename: C
authors.initials: C
authors.lastname: Lacombe
mesh_terms: D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D004921:Erythropoietin / Q000378:metabolism*; D005944:Glucosamine / Q000494:pharmacology; D006031:Glycosylation; D051379:Mice; D008970:Molecular Weight; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017467:Receptors, Erythropoietin; D017026:Swainsonine; D014415:Tunicamycin / Q000494:pharmacology
chemical_list: D000470:Alkaloids; D011956:Receptors, Cell Surface; D017467:Receptors, Erythropoietin; D014415:Tunicamycin; D004921:Erythropoietin; D005944:Glucosamine; D017026:Swainsonine