Human lupus-type inclusions in umbilical cord blood mononuclear cells.
Mononuclear cells purified from umbilical cord blood activated oligoadenylate synthetase and formed human lupus-type inclusions (LI) when cultured with the purified recombinant human leukocyte interferon, IFLrA. LI frequencies increased from 0% to a low of 0.75% and a high of 6.25% in 4-day cultures with IFLrA (100 units/ml). These interferon-induced responses in the mononuclear cells of neonates indicate that LI are solely an intrinsic product of normal cells, and not an exogenous virus or some other environmental agent.
Rich
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Laboratory of Biophysics, Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
Gibbons
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D015088:2',5'-Oligoadenylate Synthetase / Q000378:metabolism; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D002479:Inclusion Bodies / Q000648:ultrastructure; D007231:Infant, Newborn; D007370:Interferon Type I / Q000494:pharmacology; D008177:Lupus Vulgaris / Q000473:pathology; D009000:Monocytes / Q000201:enzymology; D011994:Recombinant Proteins
D007370:Interferon Type I; D011994:Recombinant Proteins; D015088:2',5'-Oligoadenylate Synthetase