title: Cellular and subcellular localization of protein kinase C in cat visual cortex.
abstract: Polyclonal antibodies against 3 protein kinase C (PKC) subtypes (I, II and III) were applied to localize the kinase in cat visual cortex. These antibodies exclusively stained neuronal cells. Both pyramidal and non-pyramidal cells exhibiting PKC-like immunoreactivity were concentrated in layers, II, III, V and VI with relatively few cells in layer IV. Electron microscopic examination did not reveal any presynaptic localization of the kinase. PKC immunoreactivity remained normal in a zone of cortex surgically isolated from the rest of the brain by an undercut procedure. These results suggest that PKC is heterogenously distributed in adult cat visual cortex; the kinase recognized by the polyclonal antibodies is localized postsynaptically in intracortical neurons of the superficial and deep cortical layers and the expression of the kinase is not regulated by extracortical input.
authors.affiliation: Department of Ophthalmology, University of British Columbia, Vancouver, Canada.
authors.forename: W G
authors.initials: WG
authors.lastname: Jia
authors.forename: C
authors.initials: C
authors.lastname: Beaulieu
authors.forename: F L
authors.initials: FL
authors.lastname: Huang
authors.forename: M S
authors.initials: MS
authors.lastname: Cynader
mesh_terms: D000818:Animals; D002415:Cats; D007527:Isoenzymes / Q000032:analysis*; D008854:Microscopy, Electron; D009474:Neurons / Q000201:enzymology*; D011493:Protein Kinase C / Q000032:analysis*; D013347:Subcellular Fractions / Q000201:enzymology*; D014793:Visual Cortex / Q000166:cytology / Q000201:enzymology*
chemical_list: D007527:Isoenzymes; D011493:Protein Kinase C