Transfection of a T-cell line with neo increases dexamethasone cytotoxicity.
Glucocorticoids (GC) are important therapeutic agents used in the treatment of lymphoid malignancies. GC-mediated cytotoxicity is preceded by the activation of a lysis mechanism induced by DNA nucleosomal cleavage. We have found that transfection of a relatively GC-resistant human T-cell leukemia line with the neo gene, and subsequent selection in geneticin (G418), is associated with enhanced GC-mediated DNA cleavage and cytotoxicity. The conversion of a GC-resistant clone to cells that are GC-sensitive may have implications for experiments involving G418 selection, as well as therapeutic implications in the development of certain types of GC-resistance.
de Cuevillas
F
F
Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461.
Lachman
H
H
D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology*; D006801:Humans; D019868:Kanamycin Kinase; D010770:Phosphotransferases / Q000235:genetics*; D013601:T-Lymphocytes / Q000187:drug effects*; D014162:Transfection
D003907:Dexamethasone; D010770:Phosphotransferases; D019868:Kanamycin Kinase