Establishment of a SV40-transformed cell line from primary culture of rat dorsolateral prostatic epithelial cells.
A permanent cell line of the rat dorsolateral prostate epithelium was established after transformation of primary cultured cells with simian virus 40 (SV40). The established cells had SV40 T-antigen in their nuclei but lacked such typical characteristics of transformed cells as piled-up growth. They grew in a monolayer with an epithelial morphology, were stained with antikeratin antisera, and also retained an ability to form dome-like structure at a confluent state.
Nakahara
M
M
Department of Urology, Hiroshima University School of Medicine, Japan.
Fukushima
M
M
Tachyo
T
T
Usui
T
T
Ide
T
T
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000032:analysis / Q000276:immunology; D002461:Cell Line, Transformed; D002467:Cell Nucleus / Q000737:chemistry / Q000276:immunology; D002472:Cell Transformation, Viral / Q000276:immunology / Q000502:physiology*; D002478:Cells, Cultured; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D008297:Male; D011467:Prostate / Q000166:cytology* / Q000276:immunology / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D013539:Simian virus 40 / Q000276:immunology / Q000502:physiology*
D000952:Antigens, Polyomavirus Transforming