title: A processing enzyme for prorenin in mouse submandibular gland. Purification and characterization.
abstract: Renin is produced from an inactive precursor, prorenin, through proteolytic cleavage at paired basic amino acid residues. In this study, an enzyme which specifically cleaves mouse Ren 2 prorenin at the paired basic residues has been purified from mouse submandibular gland by CM-Toyopearl chromatography, antipain-Sepharose chromatography, and isoelectric focusing. This enzyme, named prorenin converting enzyme, consists of two polypeptide chains of 17 and 10 kDa. The enzyme has an isoelectric point of 9.5-9.8, and its pH optimum is between 7.5 and 8.5. It specifically cleaves the peptide bond on the carboxyl side of the Arg at the Lys-Arg pair of mouse Ren 2 prorenin to yield mature renin but does not cleave mouse Ren 1 and human prorenins. Studies on the effects of inhibitors indicate that this enzyme is a serine protease that differs from the enzymes processing other prohormones at paired basic amino acid residues.
authors.affiliation: Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
authors.forename: W S
authors.initials: WS
authors.lastname: Kim
authors.forename: K
authors.initials: K
authors.lastname: Hatsuzawa
authors.forename: Y
authors.initials: Y
authors.lastname: Ishizuka
authors.forename: K
authors.initials: K
authors.lastname: Hashiba
authors.forename: K
authors.initials: K
authors.lastname: Murakami
authors.forename: K
authors.initials: K
authors.lastname: Nakayama
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004792:Enzyme Precursors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007525:Isoelectric Focusing; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational; D012083:Renin / Q000235:genetics / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000302:isolation & purification* / Q000378:metabolism; D013363:Submandibular Gland / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D004792:Enzyme Precursors; D012697:Serine Endopeptidases; C063570:prorenin Ren2 converting enzyme; D012083:Renin