title: Oxidative burst in human Epstein-Barr virus-transformed B-cell lines triggered by immobilized specific antigen.
abstract: Normal human B lymphocytes and Epstein-Barr virus-transformed B-cell lines can produce reactive oxygen species such as superoxide if treated with phorbol myristate acetate (PMA) or with the surface immunoglobulin cross-linking agents protein A and anti-immunoglobulin. Here, we investigated under which conditions specific antigen, the natural ligand of surface immunoglobulin, can stimulate an oxidative burst in monoclonal Epstein-Barr virus-transformed B-cell lines producing antibodies of known specificities. After a short lag time of 1-2 min, exposure to the specific antigen stimulated a prolonged oxidative burst (tmax 30-90 min), as measured by Lucigenin-enhanced, superoxide dismutase-inhibitable chemiluminescence, in the corresponding line only. The effect was induced in each line if the specific antigen was immobilized to a solid support. Except in one line in which antigen also stimulated an oxidative burst if presented at relatively high density on a soluble carrier, soluble antigen did not induce B-cell oxidase activation. This suggests that normal, non-transformed B lymphocytes also require interaction with relatively dense deposits of specific antigen for activation of their oxidase.
authors.affiliation: Institute of Clinical Immunology, Inselspital Bern, Switzerland.
authors.forename: F E
authors.initials: FE
authors.lastname: Maly
authors.forename: A
authors.initials: A
authors.lastname: Urwyler
authors.forename: R
authors.initials: R
authors.lastname: Tengler
authors.forename: M
authors.initials: M
authors.lastname: Steinitz
authors.forename: B
authors.initials: B
authors.lastname: Golding
authors.forename: A L
authors.initials: AL
authors.lastname: de Weck
mesh_terms: D000166:Acridines; D000941:Antigens / Q000276:immunology; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D002461:Cell Line, Transformed; D004854:Herpesvirus 4, Human; D006801:Humans; D008163:Luminescent Measurements; D010088:Oxidoreductases / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D013205:Staphylococcal Protein A / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D000166:Acridines; D000941:Antigens; D013205:Staphylococcal Protein A; C033472:10,10'-dimethyl-9,9'-biacridinium; D010088:Oxidoreductases; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen