Metabolic effects of platelet-activating factor in rats in vivo. Stimulation of hepatic glycogenolysis and lipogenesis.
1. The effects of platelet-activating factor (1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphocholine; PAF) on hepatic metabolism in vivo in rats were studied. 2. PAF stimulated synthesis of hepatic lipid (saponified and non-saponified) in a dose-dependent fashion and caused hypertriglyceridaemia. There was no effect of PAF on lipogenesis in isolated hepatocytes. 3. High doses of PAF also decreased hepatic glycogen. 4. All doses of PAF decreased plasma insulin, and this was accompanied by hyperglycaemia, except at the lowest dose. 5. The selective PAF-receptor antagonist L659.989 prevented the stimulation of lipogenesis, but indomethacin did not.
Evans
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RD
Nuffield Department of Anaesthetics, Radcliffe Infirmary, Oxford, U.K.
Ilic
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Williamson
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D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D005260:Female; D005663:Furans / Q000494:pharmacology; D006003:Glycogen / Q000378:metabolism*; D007213:Indomethacin / Q000494:pharmacology; D007328:Insulin / Q000097:blood; D008055:Lipids / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000494:pharmacology*; D010980:Platelet Membrane Glycoproteins; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000037:antagonists & inhibitors; D043562:Receptors, G-Protein-Coupled; D014280:Triglycerides / Q000097:blood
D001786:Blood Glucose; D005663:Furans; D007328:Insulin; D008055:Lipids; D010972:Platelet Activating Factor; D010980:Platelet Membrane Glycoproteins; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D014280:Triglycerides; C039351:platelet activating factor receptor; C054893:L 659989; D006003:Glycogen; D007213:Indomethacin