Effect of diuretics on cell potassium transport: an electron microprobe study.
To study the short-term uptake of potassium across the basolateral membrane into individual tubule cells, rubidium was used and measured by electron microprobe analysis. Changes of rubidium uptake were interpreted to reflect altered sodium entry and basolateral Na-K-ATPase activity. The effects of hydrochlorothiazide, amiloride and furosemide were determined in saline-loaded animals. Hydrochlorothiazide inhibited rubidium uptake in proximal convoluted and distal convoluted tubule cells. The effect was largest in distal convoluted tubule cells. Amiloride reduced rubidium uptake in principal cells as well as in proximal convoluted, distal convoluted and connecting tubule cells. Furosemide depressed rubidium uptake in distal convoluted tubule cells, but increased uptake in principal cells. Rubidium uptake into intercalated cells was not affected by any of the diuretics used. Hydrochlorothiazide and amiloride altered rubidium uptake also in cells not associated with the main diuretic action. These effects of hydrochlorothiazide and amiloride may be due to interference with cell transport mechanisms of Na-H and anion exchange.
Beck
F X
FX
Department of Physiology, University of Munich, Federal Republic of Germany.
Dörge
A
A
Giebisch
G
G
Thurau
K
K
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002712:Chlorides / Q000493:pharmacokinetics; D004232:Diuretics / Q000494:pharmacology*; D004577:Electron Probe Microanalysis; D007684:Kidney Tubules / Q000187:drug effects*; D007686:Kidney Tubules, Distal / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D011188:Potassium / Q000493:pharmacokinetics*; D015221:Potassium Channels / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D012413:Rubidium / Q000493:pharmacokinetics; D015222:Sodium Channels / Q000187:drug effects; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D002712:Chlorides; D004232:Diuretics; D015221:Potassium Channels; D015222:Sodium Channels; D000254:Sodium-Potassium-Exchanging ATPase; D012413:Rubidium; C032710:rubidium chloride; D011188:Potassium