Identification of a new membrane-associated polypeptide specified by the coronavirus infectious bronchitis virus.
Nucleotide sequences from the third open reading frame of mRNA D (D3) of infectious bronchitis virus (IBV) were expressed in bacteria as part of a fusion protein with beta-galactosidase. Antiserum raised in rabbits against this fusion protein immunoprecipitated from IBV-infected chick kidney or Vero cells a polypeptide of 12.4K, the size expected for a D3-encoded product. The D3 polypeptide is apparently non-glycosylated, and appears to be associated with the membrane fraction of infected cells, as judged by cell fractionation and immunofluorescence.
Smith
A R
AR
Department of Pathology, University of Cambridge, U.K.
Boursnell
M E
ME
Binns
M M
MM
Brown
T D
TD
Inglis
S C
SC
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D001483:Base Sequence; D002458:Cell Fractionation; D002462:Cell Membrane / Q000032:analysis; D003001:Cloning, Molecular; D003332:Coronaviridae / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D005455:Fluorescent Antibody Technique; D015967:Gene Expression Regulation, Viral; D001351:Infectious bronchitis virus / Q000032:analysis / Q000235:genetics* / Q000648:ultrastructure; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics*; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis / Q000235:genetics; D010957:Plasmids; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis*; D014158:Transcription, Genetic; D014709:Vero Cells; D014760:Viral Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics*
D000914:Antibodies, Viral; D004279:DNA, Viral; D008565:Membrane Proteins; D010455:Peptides; D012333:RNA, Messenger; D012367:RNA, Viral; D014760:Viral Fusion Proteins; D014764:Viral Proteins