A simple haemolytic method for quantitation of the delta endotoxin of Bacillus thuringiensis subsp. israelensis from crude samples.
A simple haemolytic assay method for quantitative estimation of the delta endotoxin of Bacillus thuringiensis subsp. israelensis from a crude preparation has been developed. The method has several advantages over mosquito-larvicidal methods of assay as it is inexpensive, highly sensitive and easier to run and can be used for performing a reasonably large number of assays rapidly with high precision and with a coefficient of variation that does not exceed 1.96%.
Majumdar
M K
MK
Central Drug Laboratory, Calcutta, India.
Sikdar
D P
DP
Sarma
A B
AB
Majumdar
S K
SK
D001413:Bacillus thuringiensis; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D001681:Biological Assay; D004731:Endotoxins / Q000032:analysis*; D006460:Hemolysin Proteins; D006461:Hemolysis; D007306:Insecticides / Q000032:analysis*; D010572:Pest Control, Biological; D012995:Solubility
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis