Distribution of intestinal mast cell proteinase in blood and tissues of normal and Trichinella-infected mice.
A sensitive and specific enzyme-linked immunosorbent assay (ELISA) was developed for mouse intestinal mast cell proteinase (IMCP). Specificity was demonstrated by the absence of immunoreactivity with extracts of isolated serosal mast cells (SMC), or with high concentrations (50 micrograms/ml) of the antigenically similar rat mast cell proteinases I or II. The small and large intestines in normal mice were the major sources of IMCP, there being little or no IMCP in non-mucosal tissues. Concentrations of IMCP in normal (non-parasitized) mice were low, but were increased 100-1000-fold intestines of mice infected 10 days earlier with Trichinella spiralis. The kinetic response of secreted IMCP into the blood of mice following infection with T. spiralis was also studied. Systemic release of IMCP coincided with the immune expulsion of adult worms from the intestine, and peak concentrations (9.45 micrograms/ml IMCP) occurred 9 days after infection. The tissue distribution of IMCP, its secretion into blood, and its enteric accumulation during parasite infection, are consistent with a mucosal mast cell (MMC) source for IMCP. The results are discussed in the context of similar findings for rat mast cell proteinase II.
Huntley
J F
JF
Moredun Research Institute, Edinburgh.
Gooden
C
C
Newlands
G F
GF
Mackellar
A
A
Lammas
D A
DA
Wakelin
D
D
Tuohy
M
M
Woodbury
R G
RG
Miller
H R
HR
D000818:Animals; D010450:Endopeptidases / Q000097:blood / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D007413:Intestinal Mucosa / Q000201:enzymology*; D008407:Mast Cells / Q000201:enzymology*; D051379:Mice; D008815:Mice, Inbred Strains; D014018:Tissue Distribution; D014235:Trichinellosis / Q000201:enzymology*
D010450:Endopeptidases