Defective V-to-J recombination of T cell receptor gamma chain genes in scid mice.
The status of T cell receptor gamma chain genes (TcR gamma) in 11 spontaneous T cell lymphomas from mice with severe combined immune deficiency (scid) was analyzed. We found that as a result of large abnormal deletions accompanying attempted site-specific V-to-J recombinations, 36 of 47 rearranged TcR gamma genes lacked the variable (V) and/or joining (J) region gene segment involved in this attempted recombination. No such deletions were found in T cell lymphomas from normal mice. We interpret our data as indicating a defective V-to-J recombination in scid lymphocytes consistent with our earlier observation of faulty D-to-J recombination in transformed scid lymphocytes (Schuler et al., Cell 1986. 46: 963). The present results further support the hypothesis that the scid mutation affects a component of the VDJ-recombinase system used in common by B and T cells to assemble antigen receptor genes.
Schuler
W
W
Fox Chase Cancer Center, Institute for Cancer Research, Philadelphia.
Schuler
A
A
Bosma
M J
MJ
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004254:DNA Nucleotidyltransferases / Q000235:genetics; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D007153:Immunologic Deficiency Syndromes / Q000235:genetics*; D008228:Lymphoma, Non-Hodgkin; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D016692:Receptors, Antigen, T-Cell, gamma-delta; D015183:Restriction Mapping; D013601:T-Lymphocytes / Q000502:physiology*; D014407:Tumor Cells, Cultured; D045567:VDJ Recombinases
D011948:Receptors, Antigen, T-Cell; D016692:Receptors, Antigen, T-Cell, gamma-delta; D004254:DNA Nucleotidyltransferases; D045567:VDJ Recombinases