Cell-mediated immune responses after immunization of healthy seronegative children with varicella vaccine: kinetics and specificity.
Humoral and cell-mediated immune responses were determined in seronegative children immunized with live attenuated Oka strain varicella vaccine. At 2 weeks after immunization, 80% of children had detectable lymphocyte proliferation to varicella-zoster virus (VZV) antigens, while only 40% had antibodies to VZV as detected by ELISA. By 6 weeks after immunization, 97% of children seroconverted, and 95% of these responded to VZV antigens in the proliferation assay. A high proportion of immunized children also responded in the proliferation assay to purified glycoproteins I, II, and III of VZV. These results indicate that most children develop a broad cell-mediated immune response to VZV antigens within weeks after immunization with varicella vaccine.
Watson
B
B
Division of Infectious Diseases and Immunology, Joseph Stokes, Jr., Research Institute, Children's Hospital of Philadelphia, PA 19104.
Keller
P M
PM
Ellis
R W
RW
Starr
S E
SE
D000914:Antibodies, Viral / Q000096:biosynthesis*; D000956:Antigens, Viral / Q000276:immunology; D019433:Chickenpox Vaccine; D002648:Child; D006023:Glycoproteins / Q000276:immunology; D014645:Herpesvirus 3, Human / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D007114:Immunization; D007700:Kinetics; D008213:Lymphocyte Activation; D014613:Vaccines, Attenuated / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D019433:Chickenpox Vaccine; D006023:Glycoproteins; D014613:Vaccines, Attenuated; D014765:Viral Vaccines