Cellular mechanisms in the superinfection exclusion of vesicular stomatitis virus.
The superinfection exclusion of VSV has been studied and found to be caused by a combination of three distinct effects on endocytosis by VSV-infected cells: first, a decreased rate of formation of endocytic vesicles as judged by an inhibition of fluid-phase uptake at 2 hr postinfection; second, a decreased rate of internalization of receptor-bound ligands, which was detected at 4 hr postinfection; and third, a competition with newly synthesized virus for occupancy of coated pits, as indicated by electron microscopy of infected cells. At the same time that fluid-phase uptake decreased, numerous uncoated invaginations were observed at the cell surface.
Simon
K O
KO
Department of Neurobiology, Anatomy, and Cell Science, University of Pittsburgh School of Medicine, Pennsylvania 15261.
Cardamone
J J
JJ
Whitaker-Dowling
P A
PA
Youngner
J S
JS
Widnell
C C
CC
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure*; D002472:Cell Transformation, Viral; D003034:Coated Pits, Cell-Membrane / Q000648:ultrastructure; D006224:Cricetinae; D004705:Endocytosis; D007700:Kinetics; D008854:Microscopy, Electron; D015163:Superinfection; D014721:Vesicular stomatitis Indiana virus / Q000472:pathogenicity* / Q000502:physiology