Phenylhydrazine mediated degradation of bovine serum albumin and membrane proteins of human erythrocytes.
Phenylhydrazine in solution has been shown to produce hydroxyl radicals, as measured by 2-deoxyribose degradation assay. In vitro incubation of bovine serum albumin with phenylhydrazine leads to extensive degradation of the former which, however, is not inhibited by hydroxyl-radical scavengers like mannitol, Tris or n-butanol. Metal chelators like EDTA, however, inhibits the breakdown of BSA. Erythrocyte ghosts incubated in vitro with phenylhydrazine also show extensive loss of membrane cytoskeletal proteins, inhibited by 5 mM EDTA but not by mannitol. In both the occasions a hydroxyl-radical mediated damage to protein takes place by a 'site-specific mechanism'. Further, such a damage to erythrocyte membrane proteins by phenylhydrazine may be related to well known action of this compound in producing accelerated aging of erythrocytes.
Chakrabarti
S
S
Department of Biochemistry, Goa Medical College, Santha Cruz, India.
Naik
A A
AA
Reddy
G R
GR
D003598:Cytoskeletal Proteins / Q000378:metabolism*; D003855:Deoxyribose / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D006878:Hydroxides; D017665:Hydroxyl Radical; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000378:metabolism*; D010659:Phenylhydrazines / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000378:metabolism*
D003598:Cytoskeletal Proteins; D006878:Hydroxides; D008565:Membrane Proteins; D010659:Phenylhydrazines; C030299:phenylhydrazine; D012710:Serum Albumin, Bovine; D017665:Hydroxyl Radical; D003855:Deoxyribose