Beta-adrenoreceptor subtype expression in human liver.
The pharmacological and gene expressions of beta 1- and beta 2-adrenoceptor subtypes (BAR1 and BAR2) were investigated in human liver by radioligand binding assays, adenylate cyclase experiments, and RNA excess solution hybridization. [125I]Cyanopinodolol, nonlabeled adrenergic agents, and BAR1/BAR2 cRNA were used as probes. The relationship between binding sites for BAR1 and BAR2 was markedly different from that between the basal mRNA expression for the two receptor subtypes. Plasma membranes as well as a microsomel-enriched fraction contained binding sites only for BAR2. The potency of BAR agonists and antagonists in stimulating adenylate cyclase activity of plasma membranes was typical of a BAR2 response. Northern blot analysis of total cellular RNA isolated from liver tissue showed hybridization of the BAR1 probe to a mRNA species of 2.5-2.6 kilobases and of the BAR2 probe to a mRNA species of 2.2-2.3 kilobases. The basal level of BAR1 mRNA was 5-fold higher than of BAR2 mRNA, as assayed by solution hybridization. No difference in BAR subtype mRNA stability was observed, as indicated by a mRNA half-life of approximately 5.5 h for both receptor subtypes. It is concluded that specific factors are involved in the steady state regulation of BAR subtype expression in human liver. This tissue contains solely BAR2 owing to a posttranscriptional block of basal BAR1 expression.
Arner
P
P
Department of Medicine, Huddinge Hospital, Sweden.
Engfeldt
P
P
Hellström
L
L
Lönnqvist
F
F
Wahrenberg
H
H
Sonnenfeld
T
T
Brönnegård
M
M
D000262:Adenylyl Cyclases / Q000032:analysis; D001262:Atenolol / Q000494:pharmacology; D001483:Base Sequence; D015152:Blotting, Northern; D002395:Catecholamines / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D004789:Enzyme Activation / Q000187:drug effects; D015870:Gene Expression; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015341:Nucleic Acid Probes; D012333:RNA, Messenger / Q000032:analysis*; D011943:Receptors, Adrenergic, beta / Q000032:analysis / Q000187:drug effects / Q000235:genetics*
D002395:Catecholamines; D015341:Nucleic Acid Probes; D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta; D001262:Atenolol; D000262:Adenylyl Cyclases; D007545:Isoproterenol