Evidence for the regulation of 3 beta-hydroxysteroid dehydrogenase messenger RNA by human chorionic gonadotrophin in luteinized porcine granulosa cells.
The effects of hCG on the abundance of messenger RNA for the steroidogenic enzyme 3 beta-hydroxysteroid dehydrogenase (3 beta-OHSD) were studied in luteinized porcine granulosa cells. There were dose-dependent increases in the mRNA for this enzyme after 8 h incubation with hCG or dibutyryl cAMP. The results suggest that LH-like hormones regulate the expression of the 3 beta-OHSD gene in luteinized tissue, and this genomic regulation is a component of the luteotrophic regulation of steroidogenesis in the ovary.
Chedrese
P J
PJ
Reproductive Biology Research Unit, Univ. Saskatchewan, Saskatoon, Canada.
Luu The
V
V
Labrie
F
F
Juorio
A V
AV
Murphy
B D
BD
D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics*; D000818:Animals; D015152:Blotting, Northern; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D003338:Corpus Luteum / Q000166:cytology*; D046508:Culture Techniques; D000242:Cyclic AMP / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D013552:Swine
D006063:Chorionic Gonadotropin; D012333:RNA, Messenger; D000242:Cyclic AMP; D015096:3-Hydroxysteroid Dehydrogenases