[Identification of depolarization-activated calcium flow in the secretory cell membrane].
The inward depolarization-activated current of the salivary gland cell membrane of the chironomus larva has been registered by the voltage-clamp method under conditions of the intracellular dialysis in the presence of the calcium transmembrane gradient only. An activation threshold of the current is about -50 mV, the current maximum is under -10 mV, reversion up to +10 mV leads to the current reduction. An increase in the extracellular Ca2+ concentration causes current amplification; the current decreases under the influence of verapamil, chlorpromazine, Mn2+ and Co2+. These results could indicate the existence of potential-dependent calcium channels in the secretory cell membrane.
Klevets
M Iu
MIu
Gural'
Z V
ZV
D000818:Animals; D001692:Biological Transport / Q000502:physiology; D002118:Calcium / Q000493:pharmacokinetics*; D015220:Calcium Channels / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000502:physiology; D002683:Chironomidae / Q000378:metabolism*; D004175:Diptera / Q000378:metabolism*; D066298:In Vitro Techniques; D012469:Salivary Glands / Q000166:cytology / Q000378:metabolism*
D015220:Calcium Channels; D002118:Calcium
Identifikatsiia aktiviruemogo depoliarizatsieĭ kal'tsievogo toka membrany sekretornykh kletok.