title: Demonstration of a new mouse mammary tumor virus locus in the genome of the mammary tumor prone strain SHN mouse.
abstract: Until this report, there have been no detailed analyses of the mouse mammary tumor virus (MMTV) loci in the mammary tumor prone strain SHN. Using a probe, which hybridizes to the env sequence of MMTV, Southern blotting of genomic DNA from the brain after digestion with EcoRI revealed 5 endogenous proviruses: Mtv-1 (4.5kb), Mtv-2 (11 kb), Mtv-8 (6.7kb), Mtv-17 (8.3kb) and a newly-found 6.5-kb fragment. F1-hybrid mice (C3H/He female x SHN male) also possessed the 6.5-kb fragment. Thus, we conclude that the 6.5-kb fragment is unique to SHN mice. Genomic DNA from mammary tumors of SHN mice showed MMTV insertions, suggesting that activation of an oncogene(s) occurred in this strain.
authors.affiliation: Department of Hygiene, Akita University School of Medicine, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Koizumi
authors.forename: M
authors.initials: M
authors.lastname: Tsukada
authors.forename: Y
authors.initials: Y
authors.lastname: Wada
authors.forename: S
authors.initials: S
authors.lastname: Kamiyama
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics* / Q000302:isolation & purification; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D015246:Deoxyribonuclease EcoRI; D004198:Disease Susceptibility; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000235:genetics* / Q000382:microbiology; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics / Q000302:isolation & purification*; D051379:Mice; D008815:Mice, Inbred Strains; D010957:Plasmids; D015183:Restriction Mapping
chemical_list: D015342:DNA Probes; D004273:DNA, Neoplasm; D004279:DNA, Viral; D004247:DNA; D015246:Deoxyribonuclease EcoRI