The N-terminal env-derived amino acids of v-rel are required for full transforming activity.
Expression of the v-rel oncogene of the reticuloendotheliosis virus, strain T (REV-T), can mediate the transformation of chicken spleen and bone marrow cells. Although the majority of the coding sequence of the v-rel oncogene is derived from the cellular rel sequence, the N- and C-terminal amino acids are coded for by remnants of the REV env gene. The resulting v-rel protein can be described as an env-rel-(out of frame env) fusion protein. Terminal deletion mutants were constructed to determine the role that env sequences play in the transforming activity of v-rel. Deletions were designed to remove only sequences of v-rel derived from former env sequence. Additional deletions removed more substantial amounts of coding sequence. Introduction of deleted genes into an REV-T based retroviral vector permitted the transforming activities to be determined. Deletion analysis indicated that the N-terminal region of pp59v-rel is required for the transforming activity, whereas as many as 100 C-terminal amino acids could be deleted without complete loss of the activity.
Garson
K
K
Department of Microbiology & Immunology, Faculty of Medicine, University of Ottawa, Ontario, Canada.
Percival
H
H
Kang
C Y
CY
D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D003434:Crossing Over, Genetic; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D020572:Oncogene Proteins v-rel; D009857:Oncogenes; D010957:Plasmids; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011533:Proviruses / Q000235:genetics; D001356:Reticuloendotheliosis virus / Q000235:genetics*; D012190:Retroviridae / Q000235:genetics*; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics*
D004279:DNA, Viral; D015345:Oligonucleotide Probes; D020572:Oncogene Proteins v-rel; D015682:Retroviridae Proteins, Oncogenic; D014759:Viral Envelope Proteins; D011505:Protein-Tyrosine Kinases