[2 components of the high-threshold calcium current possessing different sensitivities to omega-conotoxin in the membrane of pheochromocytoma cells].
Blockade of the high-threshold calcium currents by omega-conotoxin (CgTx) was investigated by patch-clamp recordings of whole-cell currents in cultured cells of pheochromocytoma PC12. CgTx (10 mumol/l) preferentially blocked only a steady-state component of the calcium current. Inactivated component was insensitive to CgTx (up to 50 mumol/l concentration). The two component nature of the high-threshold calcium current in pheochromocytoma PC12 cells was suggested.
Pogorelaia
N Kh
NKh
Savchenko
A N
AN
Verkhratskiĭ
A N
AN
Shuba
Ia M
IaM
D000310:Adrenal Gland Neoplasms / Q000503:physiopathology*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D015220:Calcium Channels / Q000187:drug effects* / Q000502:physiology; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008978:Mollusk Venoms / Q000494:pharmacology*; D010456:Peptides, Cyclic / Q000494:pharmacology*; D010673:Pheochromocytoma / Q000503:physiopathology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000502:physiology; D020866:omega-Conotoxin GVIA
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D008978:Mollusk Venoms; D010456:Peptides, Cyclic; D020866:omega-Conotoxin GVIA
Dva komponenta vysokoporogovogo kal'tsievogo toka, obladaiushchie raznoĭ chuvstvitel'nost'iu k omega-konotoksinu, v membrane kletok feokhromotsitomy.