title: Oligomerization of Friend spleen focus-forming virus (SFFV) env glycoproteins.
abstract: The glycoprotein gp55 and its processed form gp65, which are encoded by the env gene of Friend spleen focus-forming virus (SFFV), have been implicated in the initiation of the murine acute erythroleukemia induced by Friend virus (FV). Analyses of these glycoproteins by chemical crosslinking and nonreducing/reducing two-dimensional electrophoresis showed that both gp55 and gp65 exist as monomer and disulfide-bonded dimer and trimer. These oligomers could be detected in various FV-infected erythroleukemia cell lines, as well as in the spleen cells of FV-induced erythroleukemic mice, suggesting that oligomerization is an intrinsic feature of SFFV env glycoproteins.
authors.affiliation: Department of Genetics, University of Tokyo, Japan.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Yang
authors.forename: A
authors.initials: A
authors.lastname: Tojo
authors.forename: N
authors.initials: N
authors.lastname: Watanabe
authors.forename: H
authors.initials: H
authors.lastname: Amanuma
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D003432:Cross-Linking Reagents; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D009052:Leukemia Virus, Murine / Q000032:analysis*; D007942:Leukemia, Experimental; D046911:Macromolecular Substances; D051379:Mice; D008970:Molecular Weight; D013155:Spleen Focus-Forming Viruses / Q000032:analysis*; D013388:Succinimides; D014759:Viral Envelope Proteins / Q000302:isolation & purification*
chemical_list: D003432:Cross-Linking Reagents; D046911:Macromolecular Substances; D013388:Succinimides; D014759:Viral Envelope Proteins; C035759:3,3'-dithiobis(sulfosuccinimidyl propionate)