Effect of lymphokines on beta-adrenoceptor function of human peripheral blood mononuclear cells.
Pathological induced changes in beta-adrenoceptor function occur in diseases such as asthma and hypertension. The mechanism(s) of this dysfunction is at present unclear. In the present study, the effect of lymphokines on beta-adrenoceptor agonist induced cAMP production in peripheral blood mononuclear cells (PBMC) is investigated. Pre-incubation of PBMC during 20 h with interleukin-2 (IL-2, 100 u ml-1) and granulocyte/macrophage-colony stimulating factor (GM-CSF, 100 u ml-1) significantly decreases beta-adrenoceptor agonist induced cAMP production by 35 +/- 8% and 37 +/- 11% respectively. IL-3 and IL-4 do not affect beta-adrenoceptor agonist induced cAMP production in PBMC. It can be concluded that IL-2 and GM-CSF, mediators derived from T-lymphocytes, can induce beta-adrenoceptor dysfunction in PBMC.
van Oosterhout
A J
AJ
Department of Pharmacology, Faculty of Pharmacy, University of Utrecht, The Netherlands.
Nijkamp
F P
FP
D000242:Cyclic AMP / Q000096:biosynthesis / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology; D007377:Interleukin-3 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D008222:Lymphokines / Q000494:pharmacology*; D009000:Monocytes / Q000187:drug effects*; D011943:Receptors, Adrenergic, beta / Q000187:drug effects*; D016188:Receptors, Granulocyte Colony-Stimulating Factor; D011994:Recombinant Proteins / Q000494:pharmacology
D007376:Interleukin-2; D007377:Interleukin-3; D008222:Lymphokines; D011943:Receptors, Adrenergic, beta; D016188:Receptors, Granulocyte Colony-Stimulating Factor; D011994:Recombinant Proteins; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D000242:Cyclic AMP