[Transposon mutagenesis, elimination and mobilization of plasmids in nitrogen-fixating bacterium Azospirillum brasilense Sp245].
The expressed difference in the plasmid profile of A. brasilense Sp245 is registered as a result of Tn5-Mob-mutability. Integration of the vector pSUP5011 into one of the A. brasilense Sp245 plasmid and using of the Tn5-Mob transposon to mobilize the 85Md cryptic plasmid are reported. The properties of A. brasilense Sp245 with the mutant plasmids composition (surface structure, acetylene and nitrate reduction, ability to a number of carbohydrates utilization, formation of melanin, antibiotics resistance specter) have been analyzed. The transposon Tn5-Mob insertion into the 85Md plasmid resulted in isolation of a mutant excreting a melanin-like pigment into the medium. The results suppose 85Md plasmid participation in melaninogenesis.
Katsy
E I
EI
Zhuravleva
E A
EA
Panasenko
V I
VI
D015806:Azospirillum brasilense / Q000235:genetics* / Q000378:metabolism; D004251:DNA Transposable Elements; D005798:Genes, Bacterial; D009154:Mutation; D009586:Nitrogen Fixation
D004251:DNA Transposable Elements
Transpozonovyĭ mutagenez, éliminatsiia i mobilizatsiia plazmid azotfiksiruiushcheĭ bakterii Azospirillum brasilense Sp245.