Sp1 can displace GHF-1 from its distal binding site and stimulate transcription from the growth hormone gene promoter.
DNase I footprinting experiments showed that binding activities of Sp1 and of GHF-1 to its distal site on the human growth hormone gene promoter are mutually exclusive. The kinetics of GHF-1 binding were indicative of positive cooperativity. The Sp1 site did not affect promoter activity in cell-free transcription. Still, Sp1 could compensate partially for the decreased stimulation of transcription seen at low GHF-1 concentrations.
Lemaigre
F P
FP
Hormone and Metabolic Research Unit, Louvain University Medical School, Brussels, Belgium.
Lafontaine
D A
DA
Courtois
S J
SJ
Durviaux
S M
SM
Rousseau
G G
GG
D001483:Base Sequence; D001667:Binding, Competitive; D004268:DNA-Binding Proteins / Q000378:metabolism*; D003850:Deoxyribonuclease I; D005796:Genes; D013006:Growth Hormone / Q000235:genetics*; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D010903:Pituitary Gland, Anterior; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D016329:Sp1 Transcription Factor; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D016329:Sp1 Transcription Factor; D014157:Transcription Factors; D013006:Growth Hormone; D003850:Deoxyribonuclease I