Induction of normal ultrastructure by CGRP treatment in dysgenic myotubes.
The calcitonin gene-related peptide (CGRP) restores an apparent normal ultrastructure in mdg/mdg muscle cells in vitro, including a normal triadic organization which is known to be essential for excitation-contraction (E-C) coupling. However, neither slow L-type Ca2+ channel activity nor E-C coupling, which are absent in mdg/mdg muscle, were re-established. These observations suggest a potential role of CGRP (and also of cAMP as the intracellular messenger) in the morphological development of the muscle fiber.
Garcia
L
L
Groupe de Biologie et Pathologie neuromusculaires, U.153 INSERM, Paris, France.
Pinçon-Raymond
M
M
Romey
G
G
Changeux
J P
JP
Lazdunski
M
M
Rieger
F
F
D000818:Animals; D000831:Animals, Newborn; D003994:Bucladesine / Q000494:pharmacology; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology*; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D005071:Evoked Potentials / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008817:Mice, Mutant Strains; D008854:Microscopy, Electron; D009119:Muscle Contraction / Q000187:drug effects; D009132:Muscles / Q000187:drug effects / Q000503:physiopathology / Q000648:ultrastructure*; D009137:Muscular Dystrophy, Animal / Q000473:pathology*; D009210:Myofibrils / Q000648:ultrastructure*; D012016:Reference Values; D012518:Sarcomeres / Q000187:drug effects / Q000648:ultrastructure*
D015220:Calcium Channels; D003994:Bucladesine; D015740:Calcitonin Gene-Related Peptide