Rearrangement and overexpression of the gene coding for tumor antigen p53 in a rat histiocytoma AK-5.
The gene coding for the cellular tumor antigen p53 is rearranged and overexpressed in a rat histiocytoma, AK-5. The protein coded by the gene was detected by immunofluorescence and its full size was confirmed by immunoprecipitation using monoclonal antibodies against p53. Southern hybridizations with a full length cDNA probe specific for p53 indicated rearrangement of the gene. Alterations in the upstream region, which probably disrupt the normal regulatory control are suggested by the pattern obtained using a 5'-specific p53 probe in Southern hybridization.
Deshpande
G
G
Centre for Cellular and Molecular Biology, Hyderabad, India.
Kumari
A L
AL
Khar
A
A
D000818:Animals; D005455:Fluorescent Antibody Technique; D015972:Gene Expression Regulation, Neoplastic; D018219:Histiocytoma, Benign Fibrous / Q000235:genetics* / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000276:immunology / Q000648:ultrastructure
D016159:Tumor Suppressor Protein p53