[A karyotype study of the cells of the African green monkey kidney cell line 4647 cultured long term in media with different sera].
The karyological analysis of the cell line 4647 used for production of a killed vaccine to Hepatitis A virus was run in the 98th, 107th, 117th and 127th passages by the routine and C, G, and Ag methods of differential chromosome staining. A considerable balancing of the chromosome composition at 107-127 passage levels is shown. The cells of line 4647 present a significant heterogeneity, as to the number of chromosomes, and do not belong to any distinct modal class. The modal number of chromosomes ranged from 61 to 66 and from 121 to 125 for hyperdiploid and polyploid cells, respectively. The stable modal class of cells was established in the tetrasomic region, when culturing in the medium containing 10% CS from the 107th passage, and in the medium containing 10% FBS from the 117th passage, which conforms to one of the WHO requirements asserted to the substrate cells.
Isaenko
A A
AA
Nemtsov
Iu V
IuV
Tsareva
A A
AA
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured / Q000648:ultrastructure; D002522:Chlorocebus aethiops; D002871:Chromosome Banding / Q000379:methods; D003470:Culture Media; D005819:Genetic Markers; D006507:Hepatovirus / Q000276:immunology; D007621:Karyotyping; D007668:Kidney / Q000648:ultrastructure*; D013997:Time Factors; D014761:Viral Hepatitis Vaccines / Q000096:biosynthesis
D003470:Culture Media; D005819:Genetic Markers; D014761:Viral Hepatitis Vaccines
Issledovanie kariotipa kletok pochki afrikanskoĭ zelenoĭ martyshki linii 4647, dlitel'no kul'tiviruemykh v sredakh s razlichnymi syvorotkami.