Studies on the anti-tumor-promoting activity of naturally occurring substances. IV. Pd-II [(+)anomalin, (+)praeruptorin B], a seselin-type coumarin, inhibits the promotion of skin tumor formation by 12-O-tetradecanoylphorbol-13-acetate in 7,12-dimethylbenz[a]anthracene-initiated mice.
Since Pd-II [(+)anomalin, (+)praeruptorin B], a seselin-type coumarin, was found to inhibit tumor promoter induced phenomenon in vitro, the effect of Pd-II on the in vivo tumor-promoting action of 12-O-tetradecanoylphorbol-13-acetate (TPA) in 7,12-dimethylbenz[a]anthracene-initiated mouse skin was investigated. Pd-II, applied 40 min before the TPA treatment, at a dose of 10 mumol/painting, completely suppressed tumor formation up to 20 weeks of tumor promotion, without any toxicity. Besides Pd-II, various anti-tumor-promoter coumarins were found in the traditional Chinese medicine Qian-Hu, from which Pd-II was obtained. These coumarins may be useful for the development of an effective method to prevent cancer.
Nishino
H
H
Department of Biochemistry, Kyoto Prefectural University of Medicine, Japan.
Okuyama
T
T
Takata
M
M
Shibata
S
S
Tokuda
H
H
Takayasu
J
J
Hasegawa
T
T
Nishino
A
A
Ueyama
H
H
Iwashima
A
A
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D000970:Antineoplastic Agents; D002460:Cell Line; D003374:Coumarins / Q000494:pharmacology / Q000627:therapeutic use*; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005260:Female; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism; D004854:Herpesvirus 4, Human / Q000187:drug effects / Q000254:growth & development; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D010710:Phosphates / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D012878:Skin Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity*; D014775:Virus Activation / Q000187:drug effects
D000970:Antineoplastic Agents; D003374:Coumarins; D004365:Drugs, Chinese Herbal; D010710:Phosphates; D010743:Phospholipids; D015127:9,10-Dimethyl-1,2-benzanthracene; C065837:anomalin; D013755:Tetradecanoylphorbol Acetate