title: Isolation of the ERG12 gene of Saccharomyces cerevisiae encoding mevalonate kinase.
abstract: The ERG12 gene of Saccharomyces cerevisiae has been cloned by complementation of an erg12-1 mutation affecting mevalonate kinase. From the 2.8-kb insert isolated, the functional gene has been localized on a DNA fragment of 2.1 kb. The mRNA is 1.45 kb long. Gene disruption shows that the ERG12 gene is essential in yeast, both for spore germination and vegetative growth.
authors.affiliation: Laboratoire de Biochimie et Génétique des Microorganismes, Université de Poitiers, France.
authors.forename: A
authors.initials: A
authors.lastname: Oulmouden
authors.forename: F
authors.initials: F
authors.lastname: Karst
mesh_terms: D003001:Cloning, Molecular; D005800:Genes, Fungal / Q000502:physiology; D010770:Phosphotransferases / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor); D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C021796:mevalonate kinase