Effect of canine surfactant protein (SP-A) on the respiratory burst of phagocytic cells.
Cells obtained from bronchoalveolar lavage, or neutrophils of peripheral blood of dog, were incubated with the canine surfactant-associated protein A (SP-A). A significant decrease of the production of superoxide anion was observed after subsequent stimulation with phorbol-12-myristate-13-acetate (PMA) as measured by the lucigenin-dependent chemiluminescence (CL). Several other proteins used for control experiments did not decrease lucigenin-dependent CL, indicating a specific effect of SP-A on phagocytes. Treatment of SP-A with collagenase prior to incubation with neutrophils destroyed the depleting effect on oxygen radical production of PMA-stimulated cells. We propose that SP-A acts as a regulatory factor of the respiratory burst of alveolar macrophages and neutrophils in the lungs. The inhibitory effect of SP-A is down-regulated by collagenase released from stimulated alveolar macrophages.
Weber
H
H
Gesellschaft für Strahlen- und Umweltforschung, Projekt Inhalation, Neuherberg, FRG.
Heilmann
P
P
Meyer
B
B
Maier
K L
KL
D000166:Acridines; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D015922:Complement C1q / Q000502:physiology; D004285:Dogs; D006023:Glycoproteins / Q000502:physiology*; D049449:Luminescence; D008168:Lung / Q000378:metabolism; D003012:Microbial Collagenase; D009504:Neutrophils / Q000378:metabolism; D010101:Oxygen Consumption; D010586:Phagocytes / Q000378:metabolism*; D011510:Proteolipids / Q000502:physiology*; D037662:Pulmonary Surfactant-Associated Protein A; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants / Q000502:physiology*; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000166:Acridines; D006023:Glycoproteins; D011510:Proteolipids; D037662:Pulmonary Surfactant-Associated Protein A; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants; D013481:Superoxides; C033472:10,10'-dimethyl-9,9'-biacridinium; D015922:Complement C1q; D003012:Microbial Collagenase; D013755:Tetradecanoylphorbol Acetate