The growth of simian virus 40 (SV40) host range/adenovirus helper function mutants in an African green monkey cell line that constitutively expresses the SV40 agnoprotein.
The simian virus 40 T-antigen carboxy-terminal mutants, dlA2459 and dlA2475, are cell line and temperature dependent for growth and plaque formation in monkey kidney cells. Although these mutants did form plaques on BSC-1 cells at 37 degrees C, they were about fivefold less efficient for plaque formation than wild-type simian virus 40. These mutants did not grow in CV-1 cells and did not synthesize agnoprotein in those cells. CV-1 cells which constitutively express the agnoprotein were permissive for mutant plaque formation. However, late mRNAs, virion proteins, and progeny virion yields did not accumulate to wild-type levels during mutant infection of the agnoprotein-producing cells.
Stacy
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TP
Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire.
Chamberlain
M
M
Carswell
S
S
Cole
C N
CN
D000260:Adenoviruses, Human / Q000254:growth & development; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D002522:Chlorocebus aethiops; D005816:Genetic Complementation Test; D009154:Mutation; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D013539:Simian virus 40 / Q000254:growth & development*; D014709:Vero Cells; D010948:Viral Plaque Assay; D014779:Virus Replication
D000952:Antigens, Polyomavirus Transforming; D012333:RNA, Messenger; D012367:RNA, Viral