Sequential assessment of pulmonary epithelial diethylene triamine penta-acetate clearance and intrapulmonary transferrin accumulation during Escherichia coli peritonitis.
The individual roles of pulmonary capillary endothelial and alveolar epithelial permeability in the pathogenesis of the adult respiratory distress syndrome (ARDS) are unclear. We developed a method for the sequential assessment of pulmonary macromolecule accumulation and small solute clearance in vivo using a gamma camera. We measured the exponential clearance coefficient of 111In-labeled diethylene triamine penta-acetate (111In-DTPA) to assess airway clearance of small solutes. We also calculated the exponential equilibration coefficient of 111In-labeled transferrin (111In-TF) to assess intrapulmonary accumulation of transferrin. We determined these parameters in guinea pigs with Escherichia coli peritonitis and compared them with a saline-treated control group, oleic-acid-treated groups, and a group treated with low molecular weight dextran Ringer solution. The pulmonary DTPA clearance and the intrapulmonary transferrin accumulation were significantly increased in the peritonitis group (29.4 +/- 8.2 x 10(-3) min-1, p less than 0.02, and 15.1 +/- 3.1 x 10(-3) min-1, p less than 0.02) when compared with the control group (3.1 +/- 0.8 x 10(-3) min-1 and 4.5 +/- 0.5 x 10(-3) min-1). These changes developed within 5.5 h of the initial insult. Neither increased extravascular lung water nor elevated pulmonary artery and left atrial pressures were detected in the peritonitis group. The low molecular weight dextran Ringer group did not show a significant increase in the pulmonary DTPA clearance and the intrapulmonary transferrin accumulation.(ABSTRACT TRUNCATED AT 250 WORDS)
Ishizaka
A
A
Department of Medicine, Stanford University Medical Center, CA 94305-5236.
Stephens
K E
KE
Segall
G M
GM
Hatherill
J R
JR
McDougall
I R
IR
Wu
Z
Z
Raffin
T A
TA
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000378:metabolism; D002199:Capillary Permeability; D002463:Cell Membrane Permeability; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D004927:Escherichia coli Infections / Q000378:metabolism* / Q000473:pathology; D015633:Extravascular Lung Water / Q000378:metabolism; D005260:Female; D006168:Guinea Pigs; D007205:Indium Radioisotopes; D008168:Lung / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008657:Metabolic Clearance Rate; D009829:Oleic Acids / Q000009:adverse effects; D004369:Pentetic Acid / Q000493:pharmacokinetics*; D010538:Peritonitis / Q000378:metabolism* / Q000473:pathology; D014168:Transferrin / Q000493:pharmacokinetics*
D007205:Indium Radioisotopes; D009829:Oleic Acids; D014168:Transferrin; D004369:Pentetic Acid