Generation of a human hybridoma producing a pure anti-HLA-A2 monoclonal antibody.
We report the production and characterization of a human monoclonal IgM hybridoma antibody recognizing antigen HLA-A2. B lymphocytes obtained from the peripheral blood of a multiparous volunteer 1 week postpartum were transformed in vitro by Epstein-Barr virus, screened by a microlymphocytotoxicity assay, and electrofused with the heterohybridoma fusion partner, K6H6/B5. A specifically anti-A2 secreting hybridoma cell line. MBW1, was then identified and cloned. The cytotoxic IgM antibody produced showed complete correlation (r = 1.00) with the A2 antigen on a large panel of unrelated donors' lymphocytes, and no cross-reactivity with A28, Aw68, or Aw69 antigens was observed.
Wallace
E F
EF
Genelabs, Inc., Redwood City, California.
Foung
S K
SK
Bradbury
K
K
Pask
S L
SL
Grumet
F C
FC
D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004560:Electricity; D005260:Female; D015789:HLA-A2 Antigen / Q000276:immunology*; D004854:Herpesvirus 4, Human; D006801:Humans; D006825:Hybridomas / Q000276:immunology* / Q000378:metabolism; D007075:Immunoglobulin M / Q000276:immunology
D000911:Antibodies, Monoclonal; D015789:HLA-A2 Antigen; D007075:Immunoglobulin M