The alpha 2B adrenergic receptor of undifferentiated neuroblastoma x glioma hybrid NG108-15 cells, interacts directly with the guanine nucleotide binding protein, Gi2.
In membranes of undifferentiated neuroblastoma x glioma hybrid cell line NG108-15, the apparent specific binding of [3H]yohimbine measured in the presence of 1 microM noradrenaline, was increased substantially by the presence of the poorly hydrolysed analogue of GTP, guanylyl-imidodiphosphate (Gpp[NH]p) or by preincubation of membranes with antibodies against the C-terminal decapeptide of the alpha subunit of the G-protein Gi2. Such an effect was not produced by antibodies against the equivalent region of Go alpha Gi3 alpha or Gs alpha or from non-immune serum. By contrast, total specific binding of [3H]yohimbine was not modified by co-incubation with Gpp[NH]p or by preincubation with the antibodies from any of the anti-G protein antisera. These results demonstrate a direct interaction of the alpha 2B adrenergic receptor of NG108-15 cells with Gi2.
McClue
S J
SJ
Department of Biochemistry, University of Glasgow, Scotland, UK.
Milligan
G
G
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D019204:GTP-Binding Proteins / Q000276:immunology / Q000378:metabolism*; D005910:Glioma; D006165:Guanylyl Imidodiphosphate / Q000378:metabolism / Q000494:pharmacology; D006822:Hybrid Cells / Q000378:metabolism*; D007106:Immune Sera; D007074:Immunoglobulin G; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D009447:Neuroblastoma; D009638:Norepinephrine / Q000494:pharmacology; D010455:Peptides / Q000138:chemical synthesis; D011485:Protein Binding; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000378:metabolism*; D015293:Transducin / Q000378:metabolism; D010566:Virulence Factors, Bordetella / Q000494:pharmacology; D015016:Yohimbine / Q000378:metabolism
D007106:Immune Sera; D007074:Immunoglobulin G; D010455:Peptides; D011943:Receptors, Adrenergic, beta; D010566:Virulence Factors, Bordetella; D015016:Yohimbine; D006165:Guanylyl Imidodiphosphate; D019204:GTP-Binding Proteins; D015293:Transducin; D009638:Norepinephrine