Blood-to-brain sodium transport in ischemic brain edema.
Brain edema is a frequent complication of cerebral ischemia; however, its mechanism of formation is not well understood. Sodium is known to accumulate in brain during the early stages of partial ischemia. Therefore, the present studies were undertaken to determine the relation among BBB sodium transport, integrity of the BBB, and development of brain edema during the first 24 hr after the onset of cerebral ischemia. Partial cerebral ischemia was produced in gerbils by ligation of the left common carotid artery under ether anesthesia. After recovery from the anesthetic, animals were scored for the presence of symptoms, and those with scores greater than 10 of 25 (n = 87) were chosen for this study. Measurements of tissue water, sodium, and potassium contents, and brain uptake of 22Na and 3H-mannitol were made in each group at 1.5, 3, 6, 12, and 24 hr after carotid ligation. Accumulation of sodium and water in the ischemic compared with the nonischemic cerebral cortex was progressive. This edema formation was not of the vasogenic type because the permeability of the BBB to mannitol was unchanged. Blood-to-brain sodium transport was reduced by 30% to 40% at all time points in the ischemic cortex. Nevertheless, the remaining sodium transport activity appeared to play a role in the development of brain edema because Na accumulated in the tissue at a rate that was approximately the same as the rate of 22Na uptake from blood.(ABSTRACT TRUNCATED AT 250 WORDS)
Betz
A L
AL
Department of Pediatrics, University of Michigan Medical School, Ann Arbor 48109.
Ennis
S R
SR
Schielke
G P
GP
Hoff
J T
JT
D000818:Animals; D001693:Biological Transport, Active; D001812:Blood-Brain Barrier; D001929:Brain Edema / Q000209:etiology / Q000378:metabolism*; D002545:Brain Ischemia / Q000150:complications*; D004730:Endothelium, Vascular / Q000378:metabolism; D005849:Gerbillinae; D008297:Male; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium