Identification and characterization of the virion protein products of herpes simplex virus type 1 gene UL47.
We have identified two encoded proteins with an antiserum raised against a synthetic oligopeptide corresponding to amino acids 671 to 684 of the predicted protein product of gene UL47 of herpes simplex virus type 1 (HSV-1). They have apparent Mrs of 82,000 and 81,000 and are both major virion components located in the tegument. The 82/81K proteins were first detected in infected cells in minor amounts 6 h after infection at 37 degrees C but were later (from 10 h until 24 h after infection) present in large amounts. UL47 regulation was investigated using phosphonoacetic acid (PAA), an inhibitor of DNA synthesis: the amounts of the 82/81K protein synthesized were compared with those of 65KDBP, an early gene product, and 21K/22K, a true late gene product. The data showed that UL47 is regulated as a true late gene.
McLean
G
G
Medical Research Council Virology Unit, University of Glasgow, U.K.
Rixon
F
F
Langeland
N
N
Haarr
L
L
Marsden
H
H
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D007106:Immune Sera; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000138:chemical synthesis; D018139:Simplexvirus / Q000235:genetics* / Q000648:ultrastructure; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*; D014771:Virion / Q000235:genetics* / Q000648:ultrastructure
D007106:Immune Sera; D010455:Peptides; D014764:Viral Proteins; D015678:Viral Structural Proteins