Phosphorylation of acyclic nucleotide analogs HPMPA and PMEA in L1210 mouse leukemia cell extracts.
Acyclic nucleotide analogs (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine (HPMPA) and 9-(2-phosphonylmethoxyethyl)adenine (PMEA) were phosphorylated in the presence of ribonucleoside 5'-triphosphates by the crude extract from mouse leukemia cells L1210 affording the respective mono- and diphosphoryl derivatives. The donor efficiency was decreasing in the order CTP greater than UTP greater than ATP greater than GTP. The presence of an ATP regenerating system stimulated considerably the conversion of both compounds. The rate of PMEA phosphorylation was 5-times slower than that of HPMPA both with and without an ATP regenerating system.
Merta
A
A
Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague.
Veselý
J
J
Votruba
I
I
Rosenberg
I
I
Holý
A
A
D000225:Adenine / Q000031:analogs & derivatives* / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000998:Antiviral Agents / Q000378:metabolism*; D051528:Guanylate Kinases; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007939:Leukemia L1210 / Q000378:metabolism; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D009703:Nucleoside-Phosphate Kinase / Q000502:physiology; D063065:Organophosphonates; D009943:Organophosphorus Compounds / Q000378:metabolism*; D010766:Phosphorylation; D014020:Tissue Extracts / Q000378:metabolism
D000998:Antiviral Agents; D063065:Organophosphonates; D009943:Organophosphorus Compounds; D014020:Tissue Extracts; C053001:adefovir; D000255:Adenosine Triphosphate; C050127:9-(S)-(3-hydroxy-2-(phosphonomethoxy)propyl)adenine; D009703:Nucleoside-Phosphate Kinase; D051528:Guanylate Kinases; D000225:Adenine