Effect of exogenous fatty acids on lipid synthesis, marker-enzymes, and development of glial cells maintained in serum-free culture.
Glial cells were isolated from the cerebra of 7-day-old rats and maintained in culture in a chemically defined medium that favours the development of oligodendrocytes. Acetate, butyrate, or albumin-bound hexanoate, octanoate, decanoate, laurate, myristate, palmitate, oleate, linoleate, or arachidonate was added to the culture medium. The incorporation of [3H]acetate into fatty acids and cholesterol and [35S]sulphate into sulphatide, and the activities of the oligodendrocyte marker enzymes 2',3'-cyclic-nucleotide 3'-phosphodiesterase and glycerol 3-phosphate dehydrogenase were measured. The composition of the glial cell population (the number of astrocytes and oligodendrocytes) in these cultures was studied by immunocytochemistry. Results show that 1) long-chain fatty acids depress the synthesis of fatty acids, cholesterol, and sulphatide; and 2) the presence of long-chain, in contrast to short-chain, fatty acids in the culture medium lowers the activities of 2',3'-cyclic-nucleotide 3'-phosphodiesterase and glycerol 3-phosphate dehydrogenase and decreases the number of oligodendrocytes. Our results suggest that long-chain fatty acids exert a negative influence on the development of oligodendrocytes in the culture system used.
Sykes
J E
JE
Laboratory of Veterinary Biochemistry, State University of Utrecht, The Netherlands.
Lopes-Cardozo
M
M
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000032:analysis*; D000818:Animals; D001253:Astrocytes / Q000737:chemistry; D015415:Biomarkers / Q000737:chemistry; D001790:Blood Physiological Phenomena; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D005227:Fatty Acids / Q000494:pharmacology*; D005993:Glycerolphosphate Dehydrogenase / Q000032:analysis*; D007150:Immunohistochemistry; D008055:Lipids / Q000096:biosynthesis*; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009836:Oligodendroglia / Q000201:enzymology; D051381:Rats
D015415:Biomarkers; D003470:Culture Media; D005227:Fatty Acids; D008055:Lipids; D005993:Glycerolphosphate Dehydrogenase; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases