In vivo evaluation and comparison of collagen, acetylated collagen and collagen/glycosaminoglycan composite films and sponges as candidate biomaterials.
Native collagen, acetylated collagen, collagen/10% chondroitin sulphate, collagen/2.5% hyaluronic acid and collagen/20% hyaluronic acid were implanted both as film and as sponge into rat lumbar muscle for 7 and 14 d. After 7 d implantation, all materials elicited an acute inflammatory cell response characterized by numerous polymorphs and histocytes. The cell population after 14 d was principally mononuclear, i.e. leucocytes, neutrophils, macrophages, lymphocytes and fibroblasts. Both films and sponges followed a similar pattern. Native collagen elicited a subacute inflammatory response after 7 d. However, 14 d after implantation, a marked infiltration by neutrophils was apparent with subsequent degradation of existing collagen material. Acetylated collagen film evoked a much greater inflammatory cell response than native collagen. Both collagen/hyaluronic acid composites elicited a similar response. The collagen/10% chondroitin sulphate composite elicited the least inflammatory cell response at 7 d, whereas infiltration by host fibroblasts after 14 d implantation was clearly seen.
Srivastava
S
S
Devro Ltd, Moodiesburn, Chryston, Glasgow, UK.
Gorham
S D
SD
French
D A
DA
Shivas
A A
AA
Courtney
J M
JM
D000107:Acetylation; D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects; D003094:Collagen / Q000378:metabolism / Q000633:toxicity; D006025:Glycosaminoglycans / Q000378:metabolism / Q000633:toxicity; D006820:Hyaluronic Acid / Q000032:analysis; D007249:Inflammation / Q000209:etiology; D008422:Materials Testing; D003012:Microbial Collagenase / Q000378:metabolism; D009132:Muscles / Q000601:surgery; D011108:Polymers / Q000378:metabolism / Q000633:toxicity; D051381:Rats; D015503:Surgical Sponges / Q000009:adverse effects
D001672:Biocompatible Materials; D006025:Glycosaminoglycans; D011108:Polymers; C058506:collagen-glycosaminoglycan copolymer; D006820:Hyaluronic Acid; D003094:Collagen; D003012:Microbial Collagenase