title: Continuous expression and replication of the hepatitis delta virus genome in Hep G2 hepatoblastoma cells transfected with cloned viral DNA.
abstract: To establish stable cell clones allowing continuous replication of hepatitis delta virus (HDV), Hep G2, a hepatoblastoma cell line containing no hepatitis B virus (HBV) DNA sequences, was transfected with a recombinant plasmid containing a tandem trimer of HDV cDNA (driven by the simian virus 40 late promoter) and a neomycin-resistance gene. After selection with the neomycin analogue G418, at least two of the resistant clones were shown to have intact delta antigen by specific immunoblotting, and the delta antigen was located in the cell nucleus by immunofluorescence. Transfected cloned viral DNAs were found to be integrated into cell chromosomes. Replication of the HDV genome was demonstrated by the presence of not only genomic and antigenomic HDV RNAs but also HDV RNAs in multimeric and circular forms. In addition, a 0.8-kilobase antigenomic RNA containing a poly(A) tail and encoding the delta-antigen open reading frame was documented. Continuous replication and transcription of the HDV genome was thus achieved in these transfected cell lines. The results confirmed that replication of HDV was unassisted by HBV. Stable passage of such cell lines strongly suggests that HDV lacks direct cytopathicity in hepatocytes. These clones should be useful in studying the details of the HDV life cycle and the relationship between HDV and its helper virus, HBV.
authors.affiliation: Graduate Institute of Clinical Medicine, National Taiwan University, Taipei.
authors.forename: P J
authors.initials: PJ
authors.lastname: Chen
authors.forename: M Y
authors.initials: MY
authors.lastname: Kuo
authors.forename: M L
authors.initials: ML
authors.lastname: Chen
authors.forename: S J
authors.initials: SJ
authors.lastname: Tu
authors.forename: M N
authors.initials: MN
authors.lastname: Chiu
authors.forename: H L
authors.initials: HL
authors.lastname: Wu
authors.forename: H C
authors.initials: HC
authors.lastname: Hsu
authors.forename: D S
authors.initials: DS
authors.lastname: Chen
mesh_terms: D000956:Antigens, Viral / Q000032:analysis / Q000235:genetics; D015139:Blotting, Southern; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D003001:Cloning, Molecular; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D005814:Genes, Viral; D003698:Hepatitis Delta Virus / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms; D010957:Plasmids; D014162:Transfection
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral