title: ESR spin trapping and NMR spectroscopy of the same heart shows correlation between energy depression and radical formation during postischemic reperfusion.
abstract: The relevance of radical formation in disturbances of energy metabolism in the postischemic heart is not clear. This study provides the first evidence of a significant correlation between the amount of oxy-radicals trapped in the effluent of isolated hearts upon reperfusion and the decreased myocardial content of phosphocreatine and ATP. This suggests that the loss of high-energy compounds might contribute to oxy-radical production during reperfusion. The application of ESR spin trapping and of NMR technique to the same heart is a new approach to investigate the pathobiochemical relevance of free radicals for the heart muscle.
authors.affiliation: Institute of Drug Research, Academy of Sciences of the GDR, Berlin.
authors.forename: I E
authors.initials: IE
authors.lastname: Blasig
authors.forename: A Y
authors.initials: AY
authors.lastname: Steinschneider
authors.forename: V L
authors.initials: VL
authors.lastname: Lakomkin
authors.forename: A N
authors.initials: AN
authors.lastname: Ledenev
authors.forename: O V
authors.initials: OV
authors.lastname: Korchazhkina
authors.forename: E K
authors.initials: EK
authors.lastname: Ruuge
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D003327:Coronary Disease / Q000175:diagnosis / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D005609:Free Radicals; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D015425:Myocardial Reperfusion; D010725:Phosphocreatine / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D005609:Free Radicals; D010725:Phosphocreatine; D000255:Adenosine Triphosphate