Fingerprinting of Mycoplasma gallisepticum strains isolated from multiple-age layers vaccinated with live F strain.
Mycoplasma gallisepticum (MG) isolates were obtained from three multiple-age commercial layer farms on which live F strain vaccine had been administered to each replacement flock for at least 2 years. All such isolates had restriction endonuclease DNA and sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein patterns characteristic of F strain. These cultures also hybridized in dot blot assays with both the MG strain-specific and species-specific DNA probes. In contrast, the original MG isolate that came from one of the farms before vaccination began clearly was not F strain. These results suggest that continuous use of live F strain vaccine in each replacement pullet flock on multiple-age commercial layer sites will result in displacement of the original field strain of MG with the vaccine strain.
Kleven
S H
SH
Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens 30605.
Khan
M I
MI
Yamamoto
R
R
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D001428:Bacterial Vaccines; D002645:Chickens / Q000382:microbiology*; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D009174:Mycoplasma / Q000235:genetics* / Q000276:immunology; D009710:Nucleotide Mapping; D010449:Peptide Mapping; D013045:Species Specificity; D014611:Vaccination / Q000662:veterinary
D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D015342:DNA Probes; D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes