[The beta-adrenergic regulation of the Na, K-ATPase activity in the sarcolemma of the heart muscle].
Using a sensitive potentiometric method the effect of isoproterenol upon the activity of Na, K-ATPase in cardiomyocytes has been studied. The activity of the enzyme in rat sarcolemma at isoproterenol-induced myocarditis decreases by 42%. A direct action of isoproterenol on the Na, K-ATPase activity in sarcolemma in vitro has been investigated. In the concentration range 10(-9)-10(-3) M (from receptor-binding up to cardiotoxic) a gradual decrease of the activity reaching the complete inhibition at 10(-3) M is revealed. Antagonist of beta-adrenoreceptors propranolol in concentrations required for displacing the agonist (10(-9) M) provides for the recovery of the Na, K-ATPase activity up to 76% of its normal activity. This action transforms into nonspecific inhibition at rising concentration of the antagonist. Possible mechanisms of the beta-adrenergic regulation effect in cardiomyocytes on Na, K-ATPase of the sarcolemma are discussed.
Osadchaia
L M
LM
Mugula
Zh
Zh
Stefanov
V E
VE
D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006321:Heart / Q000187:drug effects; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D009205:Myocarditis / Q000139:chemically induced / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology*; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000502:physiology*; D012508:Sarcolemma / Q000187:drug effects / Q000201:enzymology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis / Q000378:metabolism*
D011943:Receptors, Adrenergic, beta; D011433:Propranolol; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase; D007545:Isoproterenol
Beta-adrenergicheskaia reguliatsiia aktivnosti Na, K-ATFazy sarkolemmy serdechnoĭ myshtsy.