title: Failure to detect viral genomic sequences of three viruses (herpes simplex, simian virus 40 and adenovirus) in human and rat brain tumors.
abstract: Little is known about oncogenesis in brain tumors. Viruses are thought to be involved in some neurological diseases, the presence of subfractions of viral DNA has been reported in various circumstances and the oncogenicity of some viruses has been demonstrated in animal experiments. The discovery of homologies between retroviral oncogenes and normal cellular genes (proto-oncogenes) has stimulated once again the search for viral responsibility in oncogenesis. Having a large bank of tumor material available, we systematically examined 39 brain tumors using Southern blot hybridization with DNAs of three viruses, known to be involved in neurological diseases: herpes simplex virus (HSV), simian virus 40 (SV40) and adenovirus type 2 (Ad2). We detected no homology between the DNAs of the examined material and the viral DNA probes. We compare these negative results with those of other published studies and discuss the experimental conditions, with special reference to the possibility of non-specific hybridization, which could account for the positive results reported. The present negative results could be interpreted either as absence of involvement of the three investigated viruses in brain tumor oncogenesis, or an indirect involvement through a hit-and-run mechanism or a highly dispersed state of the viral sequences among the host genome, which would prevent hybridization with the probe, as it has been supposed to be the case during the latency phase of herpes virus.
authors.affiliation: Laboratoire de Médecine et Chirurgie Expérimentales et Comparées, Université Joseph Fourier de Grenoble, La Tronche, France.
authors.forename: C
authors.initials: C
authors.lastname: Chauvin
authors.forename: M
authors.initials: M
authors.lastname: Suh
authors.forename: C
authors.initials: C
authors.lastname: Remy
authors.forename: A L
authors.initials: AL
authors.lastname: Benabid
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis*; D005814:Genes, Viral; D006801:Humans; D009693:Nucleic Acid Hybridization; D051381:Rats; D013539:Simian virus 40 / Q000235:genetics*; D018139:Simplexvirus / Q000235:genetics*
chemical_list: D004279:DNA, Viral