title: Stabilization of prostaglandin synthetase by immobilization of goat seminal microsomes on silica gel-G.
abstract: Prostaglandin synthetase was immobilized by adsorption of goat vesicular microsomes on silica gel containing CaSO4 (silica gel G). Repeated cycles of enzymatic conversion of arachidonic acid to prostaglandin by the immobilized microsomes increased the product yield by 1.5 fold, in comparison to the same by free microsomal particles. The presence of Ca2+ in silica gel is responsible for this improved yield of prostaglandin as the divalent metal ion stabilized prostaglandin synthetase activity in a remarkable way. Microsomal particles immobilized on solid supports like alumina G and controlled pore glass were not very effective.
authors.affiliation: Department of Chemistry, Bose Institute, Calcutta.
authors.forename: D
authors.initials: D
authors.lastname: Ghosh
authors.forename: E
authors.initials: E
authors.lastname: Mukherjee
authors.forename: J
authors.initials: J
authors.lastname: Dutta
mesh_terms: D000818:Animals; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005782:Gels; D006041:Goats; D008297:Male; D008861:Microsomes / Q000201:enzymology*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D012661:Semen; D058428:Silica Gel; D012822:Silicon Dioxide
chemical_list: D004800:Enzymes, Immobilized; D005782:Gels; D058428:Silica Gel; D012822:Silicon Dioxide; D011451:Prostaglandin-Endoperoxide Synthases