Suppression of synthesis and release of glucagon by glucagon-like peptide-1 (7-36 amide) without affect on mRNA level in isolated rat islets.
Glucagon-like peptide-1 (GLP-1) has been reported to inhibit glucagon release. To investigate the mechanism involved, we examined the effects of GLP-1 on the preproglucagon mRNA level and the content and release of glucagon in the isolated rat islets. Arginine significantly increased the content and release of glucagon after incubation for 1 h or 18 h. The preproglucagon mRNA level was not increased after incubation for 1 h but was increased after incubation for 18 h. GLP-1 (7-36 amide) significantly decreased the content and release of glucagon after incubation for 1 h or 18 h, but did not affect arginine-induced increase in the preproglucagon mRNA level after incubation for 18 h. These data suggest that GLP-1 suppresses post-transcriptional processes in the content and release of glucagon.
Yamato
E
E
Department of Geriatric Medicine, Osaka University Medical School, Japan.
Noma
Y
Y
Tahara
Y
Y
Ikegami
H
H
Yamamoto
Y
Y
Cha
T
T
Yoneda
H
H
Ogihara
T
T
Ohboshi
C
C
Hirota
M
M
D000818:Animals; D005934:Glucagon / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D052216:Glucagon-Like Peptide 1; D004763:Glucagon-Like Peptides; D066298:In Vitro Techniques; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D010446:Peptide Fragments; D010455:Peptides / Q000494:pharmacology*; D052336:Proglucagon; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains
D010446:Peptide Fragments; D010455:Peptides; D011498:Protein Precursors; D012333:RNA, Messenger; C055453:glucagon-like peptide 1 (7-36)amide; D052336:Proglucagon; D004763:Glucagon-Like Peptides; D052216:Glucagon-Like Peptide 1; D005934:Glucagon