Influence of phospholipid fatty acyl composition on sarcolemmal and sarcoplasmic reticular cation transporters.
Using solubilization/reconstitution techniques, we have investigated the influence of membrane fatty acyl composition on the activities of sarcolemmal and sarcoplasmic reticular transporters. The sarcolemmal Na(+)-Ca2+ exchanger and Na+, K(+)-ATPase and the sarcoplasmic reticular Ca2(+)-ATPase were reconstituted into phosphatidylcholine:phosphatidylserine:cholesterol (30:50:20% by weight) proteoliposomes of defined fatty acyl composition. Transport activities varied considerably with phospholipid fatty acyl composition. Quite strikingly, the dependence on membrane fatty acyl composition for all three transporters was identical.
Vemuri
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Department of Medicine, University of California, School of Medicine, Los Angeles 90024-1760.
Philipson
K D
KD
D000818:Animals; D001693:Biological Transport, Active; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D004285:Dogs; D005227:Fatty Acids / Q000378:metabolism*; D008081:Liposomes; D010743:Phospholipids / Q000378:metabolism*; D011817:Rabbits; D012508:Sarcolemma / Q000201:enzymology*; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*; D019831:Sodium-Calcium Exchanger; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D002352:Carrier Proteins; D005227:Fatty Acids; D008081:Liposomes; D010743:Phospholipids; D019831:Sodium-Calcium Exchanger; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase