title: The C gamma 1-encoded disulphide-linked and the C gamma 2-encoded nondisulphide-linked forms of the gamma/delta heterodimer use different gamma and delta variable regions.
abstract: We identified two types of gamma/delta T-cell clones: (1) BB3+ clones, which express the disulphide-linked form of the gamma/delta heterodimer (type 1 receptor) and carry rearrangements of the C gamma 1 gene; and (2) delta TCS1+ clones, which express the nondisulphide-linked form of the gamma/delta heterodimer (type 2 receptor) and carry rearrangements of the C gamma 2 gene. To determine the joining (J) and variable (V) region usage of the two receptor types, T gamma and T delta gene rearrangements and expression were analyzed in each gamma/delta T-cell clone. The results showed that the J and V regions used by the two receptor types are distinct: clones that express the type 1 receptor carry potentially functional rearrangements in J gamma P-V gamma 9 for gamma and in J delta 1-V delta 2 for delta; and clones that express the type 2 receptor had potentially functional rearrangements in J gamma 2-V gamma 2/V gamma 4 for gamma and in J delta 1-V delta 1 for delta. Therefore, two clearly different gamma/delta receptors (V gamma 9-J gamma P-C gamma 1/V delta 2-J delta 1-C delta and V gamma 2/V gamma 4-J gamma 2-C gamma 2/V delta 1-J delta 1-C delta) predominate in peripheral blood.
authors.affiliation: Istituto di Clinica Medica I, University of Perugia, Policlinico Monteluce, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Fagioli
authors.forename: E
authors.initials: E
authors.lastname: Ciccone
authors.forename: C
authors.initials: C
authors.lastname: Bottino
authors.forename: B
authors.initials: B
authors.lastname: Falini
authors.forename: F
authors.initials: F
authors.lastname: Grignani
authors.forename: A
authors.initials: A
authors.lastname: Moretta
authors.forename: L
authors.initials: L
authors.lastname: Moretta
authors.forename: P G
authors.initials: PG
authors.lastname: Pelicci
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D002999:Clone Cells; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; D004220:Disulfides / Q000378:metabolism*; D005091:Exons; D005434:Flow Cytometry; D015329:Gene Rearrangement, T-Lymphocyte; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006801:Humans; D007163:Immunosorbent Techniques; D046911:Macromolecular Substances; D009693:Nucleic Acid Hybridization; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*
chemical_list: D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D015342:DNA Probes; D004220:Disulfides; D046911:Macromolecular Substances; D011948:Receptors, Antigen, T-Cell; D004247:DNA; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific