title: Endothelin and eicosanoid synthesis in cultured mesangial cells.
abstract: We investigated the effect of endothelin on the generation of eicosanoids, which are known to regulate basal and stimulated mesangial cell tone. The results showed that endothelin is a potent stimulus of prostaglandin E2 (PGE2), prostacyclin (PGI2), and thromboxane A2 (TxA2) synthesis by bovine mesangial cells. Percentage increases in eicosanoid synthesis induced by endothelin (10(-10) to 10(-6) M), were 50 to 275% for PGE2, 28 to 168% for PGI2 and 42 to 111% for TxA2, respectively. Endothelin-induced eicosanoid synthesis in mesangial cells was concentration, but not time dependent. Aspirin (500 microM) completely prevented endothelin-induced eicosanoid synthesis. The calcium entry blocker nitrendipine (10(-8) M) failed to inhibit endothelin-induced eicosanoid synthesis. These data suggest that endothelin-induced changes in renal circulation and glomerular function in normal and disease conditions may be modulated by the concomitant stimulation of mesangial eicosanoid synthesis.
authors.affiliation: Mario Negri Institute for Pharmacological Research, Bergamo, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Zoja
authors.forename: A
authors.initials: A
authors.lastname: Benigni
authors.forename: D
authors.initials: D
authors.lastname: Renzi
authors.forename: A
authors.initials: A
authors.lastname: Piccinelli
authors.forename: N
authors.initials: N
authors.lastname: Perico
authors.forename: G
authors.initials: G
authors.lastname: Remuzzi
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D015777:Eicosanoids / Q000096:biosynthesis*; D016232:Endothelins; D004730:Endothelium, Vascular / Q000378:metabolism; D005920:Glomerular Mesangium / Q000166:cytology / Q000378:metabolism*; D066298:In Vitro Techniques; D010455:Peptides / Q000494:pharmacology*; D013268:Stimulation, Chemical
chemical_list: D015777:Eicosanoids; D016232:Endothelins; D010455:Peptides