title: Avian adenovirus isolated from pigeons affected with inclusion body hepatitis.
abstract: Avian adenoviruses were isolated from two pigeons affected with inclusion body hepatitis (IBH) by using chicken embryo liver cell cultures. One of the isolates, designated strain S-PL1, replicated in the cell nuclei forming intranuclear inclusion bodies, showed adenovirus-like morphology by electron microscopy, and cross-reacted serologically with strain SR-48 known as serotype 2 of fowl adenovirus. The strain S-PL1 killed day-old chicks by subcutaneous inoculation, and its 50% chicken lethal dose was 10(3.8) plaque forming units per bird. Severe lesions characterized with IBH and pancreatitis, were produced in chicks inoculated with the virus. Intranuclear inclusion bodies were also recognized in the liver, pancreas, kidney, proventriculus, small intestine, and caecum. By indirect immunofluorescence test, intranuclear viral antigens were detected in the liver, pancreas and other tissues.
authors.affiliation: Chemo-Sero-Therapeutic Research Institute, Kumamoto, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Takase
authors.forename: N
authors.initials: N
authors.lastname: Yoshinaga
authors.forename: T
authors.initials: T
authors.lastname: Egashira
authors.forename: T
authors.initials: T
authors.lastname: Uchimura
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
mesh_terms: D000256:Adenoviridae / Q000302:isolation & purification*; D000818:Animals; D000259:Aviadenovirus / Q000302:isolation & purification* / Q000648:ultrastructure; D010856:Columbidae / Q000382:microbiology*; D006520:Hepatitis, Animal / Q000382:microbiology*; D007181:Inclusion Bodies, Viral / Q000648:ultrastructure*; D008854:Microscopy, Electron; D009500:Neutralization Tests; D013045:Species Specificity