title: Multiple effects of ethane dimethanesulfonate on the epididymis of adult rats.
abstract: Ethane dimethanesulfonate (EDS), a Leydig cell toxicant which results in transient infertility, was used in a 4 day postexposure experimental protocol designed to identify any effects this compound might exert on the epididymis. The techniques of efferent duct ligation and testosterone (T) implantation were used to negate the role of testicular effects on the epididymal parameters. Numerous evaluations were performed including light and electron microscopy, computer assisted sperm motion analyses, and electrophoresis of sperm membrane proteins. EDS was shown to affect the epididymis in a dose-dependent fashion. The action of EDS on the epididymis is in part due to Leydig cell cytotoxicity and the resulting decrease in circulating androgen since T implantation prevented some of the changes in sperm proteins and motility. However, neither efferent duct ligation nor T implantation prevented the formation of sperm granulomas in the caput epididymidis, the distinct morphological alterations of the corpus epididymidis, the modification of certain sperm membrane proteins, or the decrease in the progressive motility and velocity of sperm following EDS treatment. Although we cannot prove these effects of EDS are due to a direct action on the epididymis, it is now clear that EDS has a distinct action on the epididymis which is unrelated to circulating T or testicular fluid.
authors.affiliation: NSI, Inc., Research Triangle Park, North Carolina.
authors.forename: G R
authors.initials: GR
authors.lastname: Klinefelter
authors.forename: J W
authors.initials: JW
authors.lastname: Laskey
authors.forename: N R
authors.initials: NR
authors.lastname: Roberts
authors.forename: V
authors.initials: V
authors.lastname: Slott
authors.forename: J D
authors.initials: JD
authors.lastname: Suarez
mesh_terms: D000818:Animals; D004822:Epididymis / Q000187:drug effects / Q000473:pathology* / Q000648:ultrastructure; D007985:Leydig Cells / Q000187:drug effects / Q000473:pathology; D008297:Male; D008698:Mesylates / Q000633:toxicity*; D008854:Microscopy, Electron; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D013081:Sperm Motility / Q000187:drug effects; D013087:Spermatids / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D013737:Testis / Q000187:drug effects / Q000473:pathology*
chemical_list: D008698:Mesylates; C002994:ethylene dimethanesulfonate