A gas-flow cryostat for use in freeze-quench studies: design and application to discontinuous pre-steady-state spectral analyses.
A design for an improved freeze-quench apparatus is presented. A freeze-quench apparatus has two main parts: the apparatus which mixes the reactants and after a set time sprays the reacting mixture into the quencher, and the quenching apparatus itself. The quenching apparatus is the novel feature of our design and it comprises a gas-flow cryostat mounted directly onto a liquid nitrogen storage Dewar. The gas flow maintains the temperature of a small volume of isopentane, which acts as the freeze-quench agent, at -140 degrees C. (The apparatus can maintain the quenching temperature over the range -190 degrees C to room temperature.) Because of the small size of the cryostat and the much reduced volume of solvent used by this method it is more convenient to use than its predecessors, can be used in the open laboratory, and is safer. Our apparatus is designed for application to electron paramagnetic resonance studies but could be easily modified for use with other spectroscopic techniques.
Moodie
A D
AD
Department of Biochemistry and Microbiology, University of St. Andrews, Scotland, United Kingdom.
Mitchell
R H
RH
Ingledew
W J
WJ
D001671:Biochemistry / Q000295:instrumentation*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D004798:Enzymes / Q000378:metabolism; D005615:Freezing; D007700:Kinetics; D010420:Pentanes / Q000737:chemistry; D013057:Spectrum Analysis
D004798:Enzymes; D010420:Pentanes; C067038:isopentane