Displacement chromatography on cyclodextrin-silicas. III. Enantiomer separations.
The feasibility of preparative enantiomer separations by displacement chromatography on analytical-scale beta-cyclodextrin-silica columns, operated in the reversed-phase mode, was demonstrated using the enantiomers of mephobarbital, hexobarbital, dansylleucine, dansylvaline and dansylphenylalanine as model solutes. The method development scheme (which relies on the determination of the elution-mode retention behavior and the adsorption isotherms of the solutes and the candidate displacers) described in Parts I and II can be used to select the appropriate displacers and the conditions leading to a successful displacement chromatographic separation. The importance of the displacer (both type and concentration) to the success of the displacement chromatographic separation is demonstrated.
Vigh
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Chemistry Department, Texas A&M University, College Station 77843-3255.
Quintero
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Farkas
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D000596:Amino Acids / Q000032:analysis; D001463:Barbiturates / Q000032:analysis; D002845:Chromatography / Q000379:methods*; D003505:Cyclodextrins; D003619:Dansyl Compounds / Q000032:analysis; D003912:Dextrins; D012822:Silicon Dioxide; D013213:Starch; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
D000596:Amino Acids; D001463:Barbiturates; D003505:Cyclodextrins; D003619:Dansyl Compounds; D003912:Dextrins; D047392:beta-Cyclodextrins; D012822:Silicon Dioxide; D013213:Starch; C031215:betadex