Establishment of a karyotypically normal B-chronic lymphocytic leukemia cell line; evidence of leukemic origin by immunoglobulin gene rearrangement.
A new Epstein-Barr virus (EBV) transformed cell line was established from a patient with B-chronic lymphocytic leukemia (B-CLL). The karyotype of the cell line has remained normal for over 12 months in culture; however, identical heavy and light chain immunoglobulin (Ig) gene rearrangements in the patient's blood and the cell line provided evidence that the EBV transformed cells were derived from the neoplastic clone.
Saltman
D
D
Medical Research Council Human Genetics Unit, Edinburgh, Scotland.
Bansal
N S
NS
Ross
F M
FM
Ross
J A
JA
Turner
G
G
Guy
K
K
D000368:Aged; D015139:Blotting, Southern; D002472:Cell Transformation, Viral; D005260:Female; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D007621:Karyotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D010641:Phenotype; D014407:Tumor Cells, Cultured