Neural transplantation using temperature-sensitive immortalized neural cells: a preliminary report.
Current approaches to neural transplantation--adrenal medullary and fetal brain--suffer from limitations in tissue availability, cellular uniformity, and general applicability. The system described here uses temperature-sensitive immortalized clonal neural cells to address these limitations. Cerebellar cells, immortalized with a temperature-sensitive tumor virus, are transformed at 34 degrees C, but differentiate at 38 degrees C. The cells were genetically engineered to express beta-galactosidase, providing a tag to study their fate in the host. The cells were then successfully transplanted to the cerebellum of adult rats.
Bredesen
D E
DE
University of California, San Francisco.
Hisanaga
K
K
Sharp
F R
FR
D000818:Animals; D001358:Avian Sarcoma Viruses; D002460:Cell Line; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000637:transplantation*; D046508:Culture Techniques / Q000379:methods*; D006085:Graft Survival; D051381:Rats