title: Polyamines and basic proteins stimulate activation by cAMP and catalytic activity of Mucor rouxii cAMP-dependent protein kinase.
abstract: Partial activation of Mucor rouxii cAMP-dependent protein kinase by cAMP was obtained when kemptide was used as substrate, but complete activation was attained with cAMP plus protamine or histone. Full activation could not be achieved by increasing kemptide or cAMP concentration. Complete activation by cAMP could be obtained by addition of 10 microM polylysine, 10 microM lysine-rich histone or 0.5 mM spermine plus spermidine. The degree of stimulation could be up to 5-fold, depending on the amount of enzyme in the assay. The same concentrations of polycations increased 1.5-2.3-fold the Vmax of kemptide phosphorylation by the free catalytic subunits of both Mucor and bovine heart protein kinases; 10 microM polyarginine inhibited completely the activity of both enzymes.
authors.affiliation: Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Ciudad, Universitaria-Pebellón, Buenos Aires, Argentina.
authors.forename: M
authors.initials: M
authors.lastname: Guthmann
authors.forename: R
authors.initials: R
authors.lastname: Pastori
authors.forename: S
authors.initials: S
authors.lastname: Moreno
mesh_terms: D000818:Animals; D002417:Cattle; D000242:Cyclic AMP / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D066298:In Vitro Techniques; D007700:Kinetics; D009089:Mucor / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology; D011073:Polyamines / Q000494:pharmacology; D011494:Protein Kinases / Q000378:metabolism*; D011506:Proteins / Q000494:pharmacology; D013379:Substrate Specificity
chemical_list: D011073:Polyamines; D011506:Proteins; D000242:Cyclic AMP; D011494:Protein Kinases