Complete nucleotide sequence of the matrix gene of human parainfluenza type 2 virus and expression of the M protein in bacteria.
The sequence of the M gene of human parainfluenza virus type 2 (PIV-2) has been determined. The sequence contained a large open reading frame with 1131 nucleotides encoding a protein with a calculated molecular weight of 42,312. Comparison of M protein sequence indicated that PIV-2 was more closely related to mumps virus and Newcastle disease virus than to other parainfluenza viruses, Sendai virus (SV), and parainfluenza virus type 3 (PIV-3), indicating a possible subdividing of the Paramyxovirus into two groups. This grouping is consistent with that obtained from analysis of the HN gene. Measles virus and canine distemper virus definitely belong to the subgroup composed of SV and PIV-3. No homology region was found in all the paramyxoviruses compared. However, a tertiary structure may be conserved in each subgroup of paramyxovirus. The M protein of PIV-2 was expressed in bacteria, and the product was recognized by a monoclonal antibody specific for the PIV-2 M protein. The bacterial-expressed protein, however, was heterogeneous and smaller in size.
Kawano
M
M
Department of Microbiology, Mie University School of Medicine, Japan.
Bando
H
H
Ohgimoto
S
S
Okamoto
K
K
Kondo
K
K
Tsurudome
M
M
Nishio
M
M
Ito
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D003001:Cloning, Molecular; D004926:Escherichia coli; D005814:Genes, Viral; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010223:Parainfluenza Virus 2, Human / Q000235:genetics*; D010252:Paramyxoviridae / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D014763:Viral Matrix Proteins / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics
D011994:Recombinant Proteins; D014763:Viral Matrix Proteins; D015678:Viral Structural Proteins