Characterization of a nucleic acid probe for the diagnosis of human coronavirus 229E infections.
A cDNA copy of the HCV229E nucleocapsid protein gene was isolated and characterized. Sequence analysis predicts a nucleocapsid polypeptide of 389 amino acids with a molecular weight (mol. wt.) of 43,450. Single strand RNA probes derived from the cDNA copy hybridize specifically to HCV229E RNA and approximately 50 pg of intracellular viral RNA can be readily detected. The application of nucleic acid hybridization as a routine procedure for the diagnosis of HCV229E infection is discussed.
Myint
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MRC Common Cold Unit, Salisbury, England.
Harmsen
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Raabe
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Siddell
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D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002213:Capsid / Q000235:genetics; D003332:Coronaviridae / Q000235:genetics*; D003333:Coronaviridae Infections / Q000175:diagnosis*; D004247:DNA / Q000235:genetics; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D011237:Predictive Value of Tests; D015347:RNA Probes / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification*; D014758:Viral Core Proteins / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics
D015347:RNA Probes; D012367:RNA, Viral; D014758:Viral Core Proteins; D015678:Viral Structural Proteins; D004247:DNA