Enhancement of anchorage-independent growth of a human adrenal carcinoma cell line by endogenously produced basic fibroblast growth factor.
We have previously observed that a human adrenal carcinoma cell line (SW-13) produces autocrine growth factors that enhance the ability of these cells to clone in soft agar. We now show that a basic FGF-like protein is found in SW-13 lysates and stimulates SW-13 colony growth. Basic FGF (bFGF) was identified in SW-13 lysates by both its chromatographic behavior on heparin-Sepharose and its reactivity with an antibody specific for bFGF. Incubation of lysates with antibodies specific for bFGF abolished their ability to support anchorage-independent growth. Northern analysis demonstrated that the cells produced multiple bFGF mRNA transcripts. SW-13 cells also expressed bFGF cell-surface receptors. These data suggest that SW-13 cells produce and respond to a factor structurally and functionally related to bFGF. Such findings support recent evidence indicating that FGF secreted by human tumor cell lines plays a role in the maintenance of the transformed phenotype.
Corin
S J
SJ
Cancer Center, University of Maryland, Baltimore, MD 21201.
Chen
L C
LC
Hamburger
A W
AW
D000310:Adrenal Gland Neoplasms / Q000378:metabolism* / Q000473:pathology; D000906:Antibodies / Q000276:immunology; D015152:Blotting, Northern; D002455:Cell Division / Q000187:drug effects*; D003115:Colony-Stimulating Factors / Q000276:immunology; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D008954:Models, Biological; D014411:Neoplastic Stem Cells / Q000378:metabolism; D012333:RNA, Messenger / Q000302:isolation & purification; D011956:Receptors, Cell Surface / Q000032:analysis; D017468:Receptors, Fibroblast Growth Factor; D014407:Tumor Cells, Cultured / Q000378:metabolism
D000906:Antibodies; D003115:Colony-Stimulating Factors; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017468:Receptors, Fibroblast Growth Factor; D005346:Fibroblast Growth Factors