title: Inactivation of the thymidine kinase gene of a gI deletion mutant of pseudorabies virus generates a safe but still highly immunogenic vaccine strain.
abstract: In an earlier report, we described the construction of the genetically engineered pseudorabies virus strain 2.4N3A which does not express glycoprotein gI. Although this strain showed a strongly reduced virulence in 10-week-old seronegative pigs, it could still cause severe disease or death in 3-day-old piglets. To attenuate the strain further, we constructed mutants with a deletion in the viral thymidine kinase gene. One mutant strain, designated 783, has a deletion of 19 base pairs and was shown to be highly immunogenic and safe for vaccination of pigs against pseudorabies virus.
authors.affiliation: Department of Virology, Central Veterinary Institute (CDI), Lelystad, The Netherlands.
authors.forename: R J
authors.initials: RJ
authors.lastname: Moormann
authors.forename: T
authors.initials: T
authors.lastname: de Rover
authors.forename: J
authors.initials: J
authors.lastname: Briaire
authors.forename: B P
authors.initials: BP
authors.lastname: Peeters
authors.forename: A L
authors.initials: AL
authors.lastname: Gielkens
authors.forename: J T
authors.initials: JT
authors.lastname: van Oirschot
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002872:Chromosome Deletion; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005814:Genes, Viral; D011558:Herpesvirus 1, Suid / Q000201:enzymology / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D015183:Restriction Mapping; D013552:Swine; D013937:Thymidine Kinase / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*; D014765:Viral Vaccines
chemical_list: D004279:DNA, Viral; D015345:Oligonucleotide Probes; D015678:Viral Structural Proteins; D014765:Viral Vaccines; D013937:Thymidine Kinase