title: Distribution of a gelsolin-like 74,000 mol. wt protein in neural and endocrine tissues.
abstract: A Ca2(+)-dependent actin binding protein with a molecular weight of 74,000, was purified from bovine adrenal medulla by using deoxyribonuclease I affinity chromatography followed by ion-exchange chromatography and gel filtration. This protein broke actin filaments into fragments and promoted nucleation of actin polymerization in a Ca2(+)-dependent manner as effectively as gelsolin. Proteolytic and immunological comparison with gelsolin which is widely distributed actin-severing protein, indicated that the 74,000 mol. wt protein is a distinct protein, but its domain structure resembles that of gelsolin. Immunoblotting using antibody against this protein showed a tissue-specific distribution. The protein was detected in various endocrine, neuroendocrine and nervous tissues, but not in muscle tissues and plasma which contained relatively large amounts of cytoplasmic and plasma gelsolin. This fact might indicate that this actin-severing protein is involved in the regulation of the secretory process of endocrine and nervous tissues. In the exocytotic process regulated by Ca2+, this protein probably plays a role to free secretory organelles like vesicles from the cytoskeletal network, mainly F-actin, which prevents the movement of secretory vesicles in the resting state.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sakurai
authors.forename: K
authors.initials: K
authors.lastname: Ohmi
authors.forename: H
authors.initials: H
authors.lastname: Kurokawa
authors.forename: Y
authors.initials: Y
authors.lastname: Nonomura
mesh_terms: D000199:Actins / Q000378:metabolism; D000313:Adrenal Medulla / Q000378:metabolism*; D000818:Animals; D002135:Calcium-Binding Proteins / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D002417:Cattle; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D018260:Gelsolin; D015151:Immunoblotting; D008840:Microfilament Proteins / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D008863:Microspheres; D008970:Molecular Weight; D009417:Nerve Tissue / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000302:isolation & purification / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D000199:Actins; D002135:Calcium-Binding Proteins; D018260:Gelsolin; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins; D010447:Peptide Hydrolases