Solubilization of the plasmin receptor from human carcinoma cells.
We could solubilize the plasmin receptor from the human colonic tumor cell line SW1116, using various detergents. Among these, Triton X100 and Sodium dodecyl sulfate were the most efficient. The solubilized receptor retained its plasmin binding activity, as judged by dot blot tests. After electrophoresis of the solubilisate in SDS polyacrylamide gels and transfer to nitrocellulose, specific plasmin fixation was still observed on autoradiograms as a sharp band in the 50K area. This band was not altered by treatment of the solubilisate with beta-mercaptoethanol.
Burtin
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UPR 277 CNRS, Groupe de Laboratoires de l'I.R.S.C., Villejuif, France.
Fondaneche
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D001345:Autoradiography; D003110:Colonic Neoplasms / Q000378:metabolism; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D005341:Fibrinolysin / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D011956:Receptors, Cell Surface / Q000302:isolation & purification*; D018000:Receptors, Peptide; D012995:Solubility; D014407:Tumor Cells, Cultured
D003902:Detergents; D011956:Receptors, Cell Surface; D018000:Receptors, Peptide; C065181:plasmin receptor; D005341:Fibrinolysin