title: Control of the interferon-induced 68-kilodalton protein kinase by the HIV-1 tat gene product.
abstract: The tat-responsive region (TAR) of the human immunodeficiency virus-1 (HIV-1) exhibits a trans-inhibitory effect on translation in vitro by activating the interferon-induced 68-kilodalton protein kinase (p68 kinase). Productive infection by HIV-1 was shown to result in a significant decrease in the amount of cellular p68 kinase. The steady-state amount of p68 kinase was also reduced in interferon-treated HeLa cell lines stably expressing tat, as compared to the amount of the kinase in interferon-treated control HeLa cells. Thus, the potential translational inhibitory effects of the TAR RNA region mediated by activation of p68 kinase may be downregulated by tat during productive HIV-1 infection.
authors.affiliation: Department of Biochemistry, McGill University, Montreal, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Roy
authors.forename: M G
authors.initials: MG
authors.lastname: Katze
authors.forename: N T
authors.initials: NT
authors.lastname: Parkin
authors.forename: I
authors.initials: I
authors.lastname: Edery
authors.forename: A G
authors.initials: AG
authors.lastname: Hovanessian
authors.forename: N
authors.initials: N
authors.lastname: Sonenberg
mesh_terms: D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics; D015536:Down-Regulation; D004790:Enzyme Induction; D015971:Gene Expression Regulation, Enzymologic; D015696:Gene Products, tat / Q000502:physiology*; D005814:Genes, Viral; D015756:Genes, tat; D015497:HIV-1 / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D007163:Immunosorbent Techniques; D007370:Interferon Type I / Q000494:pharmacology*; D008970:Molecular Weight; D011494:Protein Kinases / Q000096:biosynthesis / Q000235:genetics*; D015534:Trans-Activators / Q000502:physiology*; D014162:Transfection; D054322:tat Gene Products, Human Immunodeficiency Virus
chemical_list: D015696:Gene Products, tat; D007370:Interferon Type I; D015534:Trans-Activators; D054322:tat Gene Products, Human Immunodeficiency Virus; D011494:Protein Kinases; D015088:2',5'-Oligoadenylate Synthetase