The redox-cycling assay is not suited for the detection of pyrroloquinoline quinone in biological samples.
Based on the results of the so-called redox-cycling assay it has been claimed that various common foods and beverages as well as mammalian body fluids and tissues contain substantial quantities (microM) of free PQQ [M. Paz et al. (1989) in: PQQ and Quinoproteins (J.A. Jongejan and J.A. Duine, eds.) Kluwer Academic Publishers, Dordrecht, pp. 131-143 and J. Killgore et al. (1989) Science 245, 850-852]. However, by investigating samples from such sources with a biological assay of nM sensitivity, we could not confirm these claims. Analysis of the samples with procedures that proved adequate for the detection of PQQ adducts and conjugates gave equally negative results. To account for the positive response in the redox-cycling assay, as opposed to the negative results obtained by other methods, a search was made for those substances in these samples that caused the false-positive reactions. It was found that a number of commonly occurring biochemicals like ascorbic and dehydroascorbic acid, riboflavin and to a lesser extent pyridoxal phosphate, gave a positive response in the redox-cycling assay. The amounts of these interfering substances that were determined in the samples by independent methods could well explain the response. In separate experiments it was found that the effect of PQQ added to biological samples was obscured over an appreciable range of concentrations. For these reasons it must be concluded that the redox-cycling assay is not suited for the detection of PQQ in these samples. Any claims that are based on the results of this method should be disregarded.
van der Meer
R A
RA
Department of Microbiology and Enzymology, Delft Universaity of Technology, The Netherlands.
Groen
B W
BW
Jongejan
J A
JA
Duine
J A
JA
D000818:Animals; D003067:Coenzymes / Q000032:analysis*; D005189:False Positive Reactions; D005504:Food Analysis; D008722:Methods; D009206:Myocardium / Q000032:analysis; D010084:Oxidation-Reduction; D045542:PQQ Cofactor; D015363:Quinolones / Q000032:analysis* / Q000097:blood / Q000652:urine; D013552:Swine; D015003:Yeasts / Q000032:analysis
D003067:Coenzymes; D015363:Quinolones; D045542:PQQ Cofactor