[A new method of preparation of doxorubicin-in-oil suspension using ultrasonificator attached with Cuphorn].
The property of selective deposition of oily contrast medium, Lipiodol (LPD), in tumor tissue was utilized for targetting intraarterial infusion chemotherapy for hepatic cancers. For this therapy anti-cancer agents need to be suspended in LPD. In this report the new suspension device using ultrasonificator attached with Cuphorn was studied. Doxorubicin (Dx) was stable to ultrasonification for 1 hour. Ten mg/ml of Dx was mixed with LPD and this mixture was treated 2 times for 5 minutes with the ultrasonification method. This procedure was simple and sterile, as the commercially used Dx vials into which LPD was injected were set in the Cuphorn and ultrasonificated just as sealed. Microscopic examination of the suspension showed uniform dispersion of Dx particles without formation of aggregates. Dx particles were finely and regularly fragmented. In vitro the suspensions showed a gradual release of Dx from LPD to water phase. In one case with hepatocellular carcinoma received intraarterial infusion of this suspension, the size of the tumor and serum level of alpha-fetoprotein was prominently decreased. This ultrasonification method was simple and convenient to prepare Dx-in-oil suspension.
Miyoshi
K
K
Dept. of Pharmacology, Shikoku Cancer Center Hospital.
Sakamoto
S
S
Urayama
H
H
Jinno
K
K
Wada
T
T
Moriwaki
S
S
D006528:Carcinoma, Hepatocellular / Q000188:drug therapy; D004317:Doxorubicin / Q000008:administration & dosage* / Q000627:therapeutic use; D005260:Female; D006801:Humans; D007261:Infusions, Intra-Arterial; D007459:Iodized Oil / Q000008:administration & dosage*; D008113:Liver Neoplasms / Q000188:drug therapy; D008875:Middle Aged; D010316:Particle Size; D013535:Suspensions; D013678:Technology, Pharmaceutical / Q000379:methods; D014465:Ultrasonics
D013535:Suspensions; D007459:Iodized Oil; D004317:Doxorubicin