A catabolic plasmid involved in 4-methyl-o-phthalate and 4-hydroxy-iso-phthalate degradation in Pseudomonas cepacia.
Genes involved in 4-methyl-o-phthalate and 4-hydroxy-iso-phthalate catabolism reside on a 226-232 kbp catabolic plasmid termed MOP. This was confirmed by transformation and conjugation into an isogenic heat-cured (MOP-) derivative of the wild-type isolate, identified and termed Pseudomonas cepacia Pc701. Transformation confirmed the presence of Tn1 in MOP derived from Pc704, a mutant deficient in 4-methyl-o-phthalate catabolism. pCS1, a recombinant plasmid bearing MOP DNA, complemented MOP::Tn1 restoring the ability of Pc704 to grow on 4-methyl-o-phthalate. DNA-DNA hybridization using pCS1 as probe confirmed that loss of 4-methyl-o-phthalate catabolism by Pc704 was the result of Tn1 insertion into a 2.1 kbp HindIII fragment of MOP.
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School of Pure and Applied Biology, University of Wales College of Cardiff, U.K.
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D001673:Biodegradation, Environmental; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005798:Genes, Bacterial; D009154:Mutation; D010795:Phthalic Acids / Q000378:metabolism*; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics* / Q000378:metabolism; D014169:Transformation, Bacterial
D010795:Phthalic Acids; C065923:4-methyl-o-phthalate; D004262:DNA Restriction Enzymes; C042941:4-hydroxyisophthalic acid