In vivo detection of injected free radicals by Overhauser effect imaging.
Proton dynamical polarization is a method which unites the advantages of continuous wave ESR sensitivity and pulsed NMR imaging in order to localize free radicals. Injected nitroxide has been imaged for the first time in living rats. The high sensitivity of this method to oxygen is demonstrated.
Grucker
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Institut de Physique Biologique, UA du CNRS 1173, Faculté de Medecine, Université Louis Pasteur, Strasbourg, France.
D000818:Animals; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D005609:Free Radicals; D007274:Injections, Intraperitoneal; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D051379:Mice; D010100:Oxygen / Q000378:metabolism*; D011522:Protons; D051381:Rats; D011919:Rats, Inbred Strains; D012680:Sensitivity and Specificity; D013113:Spin Labels
D005609:Free Radicals; D011522:Protons; D013113:Spin Labels; D010100:Oxygen