Inhibition of phosphatidylinositol kinase by toyocamycin.
We have screened toyocamycin as an inhibitor of phosphatidylinositol kinase. It inhibited the enzyme of A431 cell membrane with an IC50 of 3.3 micrograms/ml. Adenosine and formycin A also inhibited the enzyme, but other 6 related nucleosides did not. Although orobol and 2,3-dihydroxybenzaldehyde that inhibit phosphatidylinositol kinase inhibited in situ phosphatidylinositol turnover, toyocamycin did not.
Nishioka
H
H
Institute of Microbial Chemistry, Tokyo, Japan.
Sawa
T
T
Hamada
M
M
Shimura
N
N
Imoto
M
M
Umezawa
K
K
D019870:1-Phosphatidylinositol 4-Kinase; D000241:Adenosine / Q000494:pharmacology; D001547:Benzaldehydes / Q000494:pharmacology; D002396:Catechols / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology; D005573:Formycins / Q000494:pharmacology; D015394:Molecular Structure; D010770:Phosphotransferases / Q000037:antagonists & inhibitors*; D014127:Toyocamycin / Q000494:pharmacology*
D001547:Benzaldehydes; D002396:Catechols; D005419:Flavonoids; D005573:Formycins; C067077:2,3-dihydroxybenzaldehyde; C034550:formycin; D010770:Phosphotransferases; D019870:1-Phosphatidylinositol 4-Kinase; D000241:Adenosine; D014127:Toyocamycin; C059811:orobol