Two puff-specific proteins bind within the 2.5 kb upstream region of the Drosophila melanogaster Sgs-4 gene.
The Drosophila nuclear proteins Bj6 and Bx42 characterized previously are detected in a series of developmentally active puffs on salivary gland chromosomes. Here the binding of both proteins at puff 3C11-12 containing the glue protein gene Sgs-4 is described in more detail. By deletion analysis we show that both proteins bind within a chromosomal segment containing 17-19 kb of DNA surrounding the Sgs-4 gene. They are detectable at this site during the intermoult stages, before the puff regresses in response to the moulting hormone ecdysone. If the Sgs-4 gene together with flanking DNA sequences is brought into a different chromosomal position by P element transfer, both proteins are detected at this new location. Both proteins are bound to the chromosome within the range of 2.5 kb DNA upstream of the Sgs-4 gene. A strain containing a 52 bp deletion within this region fails to bind Bx42 protein suggesting that the missing DNA, which overlaps a hypersensitive region, may be required for the binding of the Bx42 protein.
Saumweber
H
H
Institut für Entwicklungsphysiologie Universität zu Köln, Federal Republic of Germany.
Frasch
M
M
Korge
G
G
D000818:Animals; D002871:Chromosome Banding; D002875:Chromosomes / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics*; D005796:Genes; D005967:Glue Proteins, Drosophila / Q000235:genetics*; D007301:Insect Hormones / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000235:genetics*; D013997:Time Factors; D014170:Transformation, Genetic
D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D005967:Glue Proteins, Drosophila; D007301:Insect Hormones; D009687:Nuclear Proteins; D012471:Salivary Proteins and Peptides; D004247:DNA