Whole mouse nitroxide free radical pharmacokinetics by low frequency electron paramagnetic resonance.
The in vivo uptake distribution and reduction of the oxygen-sensitive nitroxide spin label PCA in the mouse monitored by low frequency electron paramagnetic resonance (EPR) spectroscopy are reported. Spectra were obtained from the head and liver regions of pentobarbital anesthetized mice during different circulatory and ventilatory conditions. Identical clearances were found in these regions during normoxia. Moderate hypoxia (10% O2-90% N2) did not significantly affect the spin label reduction rate.
Ferrari
M
M
Dipartimento di Scienze e Tecnologie Biomediche e Biometria, Università dell'Aquila, Italy.
Colacicchi
S
S
Gualtieri
G
G
Santini
M T
MT
Sotgiu
A
A
D000818:Animals; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D005609:Free Radicals; D006257:Head; D008657:Metabolic Clearance Rate; D051379:Mice; D009589:Nitrogen Oxides / Q000493:pharmacokinetics*; D013113:Spin Labels
D003497:Cyclic N-Oxides; D005609:Free Radicals; D009589:Nitrogen Oxides; D013113:Spin Labels; C032289:2,2,5,5-tetramethyl-1-pyrrolidinyloxy-3-carboxylic acid; C039900:nitroxyl