title: A 17-kd polypeptide, sensitive to bacterial collagenase, is synthesized by bone marrow macrophages.
abstract: A novel polypeptide with a molecular weight of 17 kd (17k protein) was identified in bone marrow cell cultures. The synthesis of 17k protein is elevated in cell cultures maintained under Dexter conditions, which support myelopoiesis. The predominance of macrophages in the stromal layer of these cultures and the observation that a mouse myelomonocytic cell line P388D1 is capable of synthesizing large amounts of 17k protein led us to the study of its synthesis by bone marrow macrophages. Metabolic labeling with [14C]proline and partial amino acid analysis of 17k protein demonstrated that the polypeptide contains relatively high amounts of proline and is also sensitive to degradation with bacterial collagenase. However, no hydroxyproline is detectable in 17k protein, and it is extensively degraded with bacterial collagenase. However, no hydroxyproline is detectable in 17k protein, and it is extensively degraded by proteolysis with pepsin, using conditions under which collagen triple helices are resistant to degradation, suggesting that collagen-like structures are not contained in 17k protein. This polypeptide is found predominantly in the cellular layers of bone marrow macrophage cultures. Incorporation of [14C]proline into 17k protein is diminished by increasing concentrations of colony-stimulating factor 1 (CSF-1). The 17k protein may be involved in macrophage proliferation because its synthesis is inhibited by CSF-1, which is required for the maintenance of bone marrow macrophages in vitro.
authors.affiliation: Department of Orthopaedics, University of Virginia School of Medicine, Charlottesville 22908.
authors.forename: L J
authors.initials: LJ
authors.lastname: Ruland
authors.forename: P J
authors.initials: PJ
authors.lastname: Quesenberry
authors.forename: G
authors.initials: G
authors.lastname: Balian
mesh_terms: D000818:Animals; D001419:Bacteria / Q000201:enzymology; D001853:Bone Marrow / Q000378:metabolism*; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D005260:Female; D016173:Macrophage Colony-Stimulating Factor; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D003012:Microbial Collagenase / Q000494:pharmacology*; D011392:Proline / Q000378:metabolism; D014176:Protein Biosynthesis
chemical_list: D003115:Colony-Stimulating Factors; D016173:Macrophage Colony-Stimulating Factor; D011392:Proline; D003012:Microbial Collagenase