title: Electrically stimulated rapid gene expression in the brain: ornithine decarboxylase and c-fos.
abstract: A single electroconvulsive shock (ECS) resulted in a major induction of cerebral ornithine decarboxylase (ODC) mRNA and a rapid and transient elevation of ODC enzyme activity. The proto-oncogene c-fos was also transiently induced under the same conditions. Following a rapid rise in mRNA levels, the messages for these proteins take different courses. The c-fos mRNA fell to below control levels by 1 h, while the ODC mRNA remained elevated beyond 24 h. The ECS-induced elevation of ODC enzyme activity was not abolished by adrenalectomy but was attenuated significantly by the anti-convulsant MK-801. These results imply that the induction of cerebral ODC may be neuronal activity dependent, and suggest that the ODC/polyamine system may be linked to the proposed third messenger cascade, involving c-fos, which couples cell stimulation to gene expression, resulting in long-term adaptive responses.
authors.affiliation: Department of Pharmacology, University of California, Irvine 92717.
authors.forename: N H
authors.initials: NH
authors.lastname: Zawia
authors.forename: S C
authors.initials: SC
authors.lastname: Bondy
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology; D004247:DNA; D003986:Dibenzocycloheptenes / Q000494:pharmacology; D016291:Dizocilpine Maleate; D004558:Electric Stimulation; D005786:Gene Expression Regulation; D008297:Male; D009693:Nucleic Acid Hybridization; D009955:Ornithine Decarboxylase / Q000235:genetics* / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D051381:Rats
chemical_list: D003986:Dibenzocycloheptenes; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D016291:Dizocilpine Maleate; D004247:DNA; D009955:Ornithine Decarboxylase