title: Evaluation of an enzyme immunoassay for the detection of herpes simplex virus (HSV) antigen from clinical specimens in viral transport media.
abstract: A total of 301 clinical specimens (229 culture positive and 72 culture negative) were assayed retrospectively by an enzyme immunoassay. Specimens were transported to the virus lab in viral transport media (VTM), and were inoculated into HF and A549 cell culture tubes for viral isolation with the remainder of the sample being saved at -70 degrees C. Specimens were thawed, vortexed and resuspended in 10 x Herptran concentrate and each sample was then added in duplicate to designated wells of a microtiter plate for the EIA assay. The EIA detected 147/150 (98%) culture positive specimens from symptomatic patients, 63/79 (79.7%) culture positive specimens from patients considered asymptomatic and 210/229 (91.7%) culture positive specimens overall. The EIA was negative for 70/72 (97.2%) culture negative specimens. These data suggest that the EIA test can be used with clinical specimens submitted in conventional VTM. However, VTM samples which are EIA negative, particularly with EIA values close to the EIA positive cutoff value, need to be cultured.
authors.affiliation: Diagnostic Virology Laboratory, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
authors.forename: C A
authors.initials: CA
authors.lastname: Gleaves
authors.forename: D H
authors.initials: DH
authors.lastname: Rice
authors.forename: C F
authors.initials: CF
authors.lastname: Lee
mesh_terms: D000956:Antigens, Viral / Q000032:analysis*; D002478:Cells, Cultured; D003470:Culture Media; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D018139:Simplexvirus / Q000276:immunology*
chemical_list: D000956:Antigens, Viral; D003470:Culture Media