A fast, simple reactivation method for the study of HSV-1 latency in the rabbit ocular model.
We developed a simple, fast, economical, and versatile reactivation method for the study of herpes simplex virus type 1 (HSV-1) latency in the rabbit ocular model. Intrastromal injection of sterile, deionized water induced reactivation and ocular shedding of latent HSV-1 in 21 of 27 eyes (78%) in 93% of New Zealand rabbits. Other control groups (eg, anterior chamber injection of sterile, deionized water; topical administration of sterile, deionized water; intrastromal injection of air; and intrastromal needle track), were less efficient in reactivating latent HSV-1. Although the mechanism of reactivation in this model is unknown, the reactivation signal may be related to the relative amount of corneal trauma.
Gordon
Y J
YJ
Eye and Ear Institute of Pittsburgh, Pittsburgh, Pennsylvania 15213.
Romanowski
E
E
Araullo-Cruz
T
T
D000287:Administration, Topical; D000818:Animals; D000867:Anterior Chamber; D003319:Corneal Stroma; D004195:Disease Models, Animal; D005260:Female; D007267:Injections; D007635:Keratitis, Dendritic / Q000382:microbiology*; D008722:Methods; D011817:Rabbits; D018139:Simplexvirus / Q000254:growth & development*; D014775:Virus Activation; D014867:Water / Q000008:administration & dosage
D014867:Water