Nuclear formation in a Drosophila cell-free system.
A cell-free preparation obtained from 0- to 5-h-old Drosophila melanogaster embryos induces chromatin decondensation and nuclear formation from demembranated Xenopus sperm. Newly formed nuclei have a peripheral lamina, a double membrane, and structures resembling pore complexes. Indirect immunofluorescence analyses demonstrate the association of Drosophila lamins and DNA topoisomerase II with newly assembled nuclei.
Berrios
M
M
Department of Pharmacological Sciences, School of Medicine, State University of New York, Stony Brook 11794-8651.
Avilion
A A
AA
D000818:Animals; D000911:Antibodies, Monoclonal; D002467:Cell Nucleus / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure; D002474:Cell-Free System; D002843:Chromatin; D004250:DNA Topoisomerases, Type II / Q000378:metabolism; D004331:Drosophila melanogaster; D004625:Embryo, Nonmammalian; D005455:Fluorescent Antibody Technique; D066298:In Vitro Techniques; D034882:Lamins; D008297:Male; D009685:Nuclear Envelope / Q000378:metabolism / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000378:metabolism; D013094:Spermatozoa / Q000648:ultrastructure; D014981:Xenopus
D000911:Antibodies, Monoclonal; D002843:Chromatin; D034882:Lamins; D009687:Nuclear Proteins; D004250:DNA Topoisomerases, Type II