Procholecystokinin and proenkephalin A mRNA expression is modulated by cyclic AMP and noradrenaline.
Regulation of the expression of procholecystokinin (proCCK) and proenkephalin A mRNA was studied in the human neuroblastoma cell line SK-N-MC. Cells were treated with dibutyryl-3',5'-cyclic AMP (dbcAMP), noradrenaline or isoproterenol, a beta-adrenoceptor agonist. Levels of proCCK and proenkephalin A mRNA were determined by Northern blot analysis with proCCK- and proenkephalin A-specific cRNA hybridization probes 9 h after drug treatments. ProCCK and proenkephalin A mRNA were co-expressed in SK-N-MC cells. ProCCK mRNA levels were increased 1.5-2.5 times by dbcAMP, noradrenaline and isoproterenol when compared with controls. The level of proenkephalin A mRNA increased approximately two to three times under the same drug conditions, whereas the level of N-myc mRNA did not change significantly. These results suggest that expression of proCCK and proenkephalin A mRNA may be regulated by a similar cAMP-dependent mechanism in the SK-N-MC cell line.
Monstein
H J
HJ
Department of Clinical Chemistry, Laboratory for Molecular Biology, Copenhagen, Denmark.
Folkesson
R
R
Geijer
T
T
D003994:Bucladesine / Q000494:pharmacology; D002766:Cholecystokinin / Q000235:genetics*; D000242:Cyclic AMP / Q000494:pharmacology*; D004745:Enkephalins / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D009447:Neuroblastoma / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization; D011498:Protein Precursors / Q000235:genetics*; D015347:RNA Probes; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D014407:Tumor Cells, Cultured
D004745:Enkephalins; D011498:Protein Precursors; D015347:RNA Probes; D012333:RNA, Messenger; C029992:proenkephalin; D003994:Bucladesine; C060249:procholecystokinin; D002766:Cholecystokinin; D000242:Cyclic AMP; D007545:Isoproterenol; D009638:Norepinephrine