Nucleotide sequence analyses of the genes encoding the HN, M, NP, P, and L proteins of two host range mutants of Sendai virus.
Comparative nucleotide sequence analyses of the genome of Sendai virus (strain Z) and two host range mutants, ts-f1 and F1-R, previously described revealed that the ts defect of ts-f1 can be attributed to two nucleotide exchanges in the NP gene. These exchanges lead to a single amino acid substitution. A single base pair change was found in both the P and L genes of F1-R, but not of ts-f1. Both host range mutants have the two same exchanges in the M gene. These additional mutations are discussed concerning their significance in the pantropic properties of the host range mutants.
Middleton
Y
Y
Department of Microbiology, California State University, Los Angeles 90032.
Tashiro
M
M
Thai
T
T
Oh
J
J
Seymour
J
J
Pritzer
E
E
Klenk
H D
HD
Rott
R
R
Seto
J T
JT
D000818:Animals; D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases; D015707:HN Protein / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D010222:Parainfluenza Virus 1, Human / Q000235:genetics*; D010750:Phosphoproteins / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D014758:Viral Core Proteins / Q000235:genetics; D014763:Viral Matrix Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*
D015707:HN Protein; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; C055859:P protein, Sendai virus; D010750:Phosphoproteins; D012367:RNA, Viral; D014758:Viral Core Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins; C064365:L protein, Sendai virus; D012321:DNA-Directed RNA Polymerases