Potentiation of forskolin-induced increase of cAMP by diamide and N-ethylmaleimide in rat pancreatic islets.
In isolated rat pancreatic islets, the effect of diamide and N-ethylmaleimide (NEM) on forskolin- as well as on glucagon-induced elevation of cAMP was studied. Forskolin and glucagon increased cAMP levels in batch-incubated islets. Diamide and NEM further augmented forskolin-induced increase of cAMP levels, whereas glucagon-stimulated elevation of cAMP was not affected. From our data it is likely that under the conditions of the present study, the thiols related to the Ns-protein and the catalytic unit are insensitive to oxidation and alkylation. The potentiation of forskolin-induced cAMP production in intact islet cells by diamide and NEM may be due to inactivation of the thiols related to the Ni-protein.
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Pharmazeutisches Institut, Abteilung Pharmakologie, Universität Tübingen, Germany.
Müller
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Safayhi
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Ammon
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D000818:Animals; D001391:Azo Compounds / Q000494:pharmacology*; D005576:Colforsin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000096:biosynthesis*; D003958:Diamide / Q000494:pharmacology*; D004357:Drug Synergism; D004790:Enzyme Induction / Q000187:drug effects; D005033:Ethylmaleimide / Q000494:pharmacology*; D005934:Glucagon / Q000494:pharmacology; D007515:Islets of Langerhans / Q000378:metabolism*; D051381:Rats
D001391:Azo Compounds; D003958:Diamide; D005576:Colforsin; D005934:Glucagon; D000242:Cyclic AMP; D005033:Ethylmaleimide