Probes for CpG islands on the distal long arm of the human X chromosome are clustered in Xq24 and Xq28.
We have isolated and characterized 55 EagI-containing genomic DNA clones from the distal long arm of the human X chromosome. The presence of additional sites for rare-cutter restriction enzymes and the demethylation of the corresponding genomic DNA demonstrate that at least 30 clones correspond to CpG islands of the Xq24-Xqter region. All clones were regionally mapped with a hybrid panel. The majority are in Xq28 and Xq24 (18 and 14 clones, respectively), 15 are in the Xq26-Xq27 interval, and none is in Xq25. This analysis demonstrates a nonuniform distribution of CpG islands that may reflect the distribution of coding regions in this part of the genome.
Maestrini
E
E
Istituto di Genetica Biochimica e Evoluzionistica, Pavia, Italy.
Rivella
S
S
Tribioli
C
C
Purtilo
D
D
Rocchi
M
M
Archidiacono
N
N
Toniolo
D
D
D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D002877:Chromosomes, Human; D003001:Cloning, Molecular; D006224:Cricetinae; D015342:DNA Probes; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015226:Dinucleoside Phosphates / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D006822:Hybrid Cells; D008745:Methylation; D014960:X Chromosome
D015342:DNA Probes; D004262:DNA Restriction Enzymes; D015226:Dinucleoside Phosphates; C015772:CpG dinucleotide