[Cleaning of Sendai virus-infected mice by embryo transfer technique].
Mouse embryos (8-16 cells) were collected from Sendai virus (SV)-infected mice at 5 or 7 weeks after inoculation. All donors having embryo(s) were positive when tested by CF test or ELISA for SV antibody at the time of embryo collection. Most of the morphologically normal embryos developed (90.6%, 259/286) to morulae or blastocysts after culture for 26-28 hr. A total of 76 embryos cultured were transferred to the uteri of SV-free pseudopregnant recipients. Forty-seven young were obtained from these recipients (61.8% of development rate) and 46 young were successfully reared up to 10th week of age. All the recipients and the young were negative by testing SV antibody. These results indicate that the embryo transfer technique is useful for cleaning of SV-infected mice.
Okamoto
M
M
National Institute of Radiological Sciences, Chiba-shi, Japan.
Matsushita
S
S
Matsumoto
T
T
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D004624:Embryo Transfer; D005260:Female; D051379:Mice; D008813:Mice, Inbred ICR; D010222:Parainfluenza Virus 1, Human / Q000276:immunology; D018184:Paramyxoviridae Infections / Q000276:immunology / Q000517:prevention & control*; D011247:Pregnancy
D000914:Antibodies, Viral