title: [Cultivation of hepatitis A virus in various cell lines and isolation of DNA sequences].
abstract: Using the MBB/11/5 strain originally adapted to the PLC/PRF/5 cell line, reproduction of the virus in diploid cells of human embryo fibroblasts, continuous primate lines (RAMT, FRhK-4) and human urinary bladder tumor (T-24) lines was studied. We obtained HAV DNA sequences (about 99% from vRNA) for the MBB/11/5 strain which were used as a probe for demonstration of vRNA synthesis in the infected cells.
authors.forename: V R
authors.initials: VR
authors.lastname: Farashian
authors.forename: A B
authors.initials: AB
authors.lastname: Bychenko
authors.forename: I A
authors.initials: IA
authors.lastname: Kuzaeva
authors.forename: E K
authors.initials: EK
authors.lastname: Artemov
authors.forename: T M
authors.initials: TM
authors.lastname: Khizhniakova
authors.forename: V M
authors.initials: VM
authors.lastname: Zhdanov
mesh_terms: D000818:Animals; D002460:Cell Line; D004247:DNA / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D006507:Hepatovirus / Q000235:genetics / Q000254:growth & development*; D006801:Humans; D009693:Nucleic Acid Hybridization; D009710:Nucleotide Mapping; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping; D014776:Virus Cultivation / Q000379:methods
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D004247:DNA
vernacular_title: Kul'tivirovanie virusa gepatita A v razlichnykh kletochnykh liniiakh i poluchenie DNK-posledovatel'nosteĭ.