Strategy for statistical-mapping of potential regulatory regions in the human genome.
We have explored the feasibility of using crude nuclear extracts and band-shift experiments, to select and clone short human DNA fragments that contain binding sites for sequence-specific nuclear proteins. We show that this strategy is feasible. It provides a means to systematically identify the recognition sequences of proteins found in nuclear extracts prepared from various tissues, in order to compile a protein-binding-sequence catalogue. A complete catalogue would provide the data needed for searching the "raw" human DNA sequences for occurrence of protein-binding sites and thus, to construct a statistical map of potential regulatory regions in the human genome.
Ambrose
C
C
Purdue University, Department of Chemistry, W. Lafayette, IN 47907.
Bina
M
M
D001483:Base Sequence; D002457:Cell Extracts; D002467:Cell Nucleus; D002874:Chromosome Mapping / Q000379:methods*; D003001:Cloning, Molecular; D004279:DNA, Viral; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015894:Genome, Human; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics
D002457:Cell Extracts; D004279:DNA, Viral; D004268:DNA-Binding Proteins