Identification and cloning of genes involved in anaerobic sulfite reduction by Salmonella typhimurium.
Transposon Tn5 insertions causing anaerobic cysteine auxotrophy were isolated from a Salmonella typhimurium cysI parent (auxotrophic under aerobic but not anaerobic conditions). Insertions in one mutant group appeared to be in cysG. A second group of insertions, designated asr (anaerobic sulfite reduction), were located near map unit 53 on the S. typhimurium chromosome. They did not cause aerobic or anaerobic auxotrophy in a cys1+ background but did prevent dissimilatory sulfite reduction. Plasmids containing asr DNA cloned from wild-type S. typhimurium conferred anaerobic prototrophy and the ability to produce hydrogen sulfide from sulfite on an Escherichia coli cys1 mutant.
Huang
C J
CJ
Department of Food Science and Technology, University of California, Davis 95616.
Barrett
E L
EL
D000693:Anaerobiosis; D003001:Cloning, Molecular / Q000379:methods; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005838:Genotype; D009154:Mutation; D010084:Oxidation-Reduction; D010641:Phenotype; D012486:Salmonella typhimurium / Q000235:genetics* / Q000378:metabolism; D013447:Sulfites / Q000378:metabolism*
D004251:DNA Transposable Elements; D013447:Sulfites