Cloning and expression of human and rat D1 dopamine receptors.
The importance of the dopaminergic system in brain function has been emphasized by its association with neurological and psychiatric disorders such as Parkinson's disease and schizophrenia. On the basis of their biochemical and pharmacological characteristics, dopamine receptors are classified into D1 and D2 subtypes. As the most abundant dopamine receptor in the central nervous system, D1 receptors seem to mediate some behavioural responses, modulate activity of D2 dopamine receptors, and regulate neuron growth and differentiation. The D dopamine receptor has been cloned by low-stringency screening. We report here the cloning of human and rat D1 dopamine receptors by applying an approach based on the polymerase chain reaction. The cloned human D1 dopamine receptor has been characterized on the basis of four criteria: the deduced amino-acid sequence, which reveals that it is a G protein-coupled receptor; the tissue distribution of its messenger RNA, which is compatible with that of the D1 dopamine receptor; its pharmacological profile when transfected into COS-7 cells; and its ability to stimulate the accumulation of cyclic AMP in human 293 cells.
Zhou
Q Y
QY
Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, Portland 97201.
Grandy
D K
DK
Thambi
L
L
Kushner
J A
JA
Van Tol
H H
HH
Cone
R
R
Pribnow
D
D
Salon
J
J
Bunzow
J R
JR
Civelli
O
O
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D002460:Cell Line; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003001:Cloning, Molecular; D003342:Corpus Striatum / Q000032:analysis; D000242:Cyclic AMP / Q000096:biosynthesis; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011954:Receptors, Dopamine / Q000235:genetics* / Q000502:physiology; D017447:Receptors, Dopamine D1; D014018:Tissue Distribution; D014162:Transfection
D012333:RNA, Messenger; D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1; D004247:DNA; D000242:Cyclic AMP