Absence of activating transmembrane mutations in the c-erbB-2 proto-oncogene in human breast cancer.
The rat neu proto-oncogene, which is a putative growth factor receptor closely related to the epidermal growth factor receptor, can be activated in vivo by a single point mutation in the sequence encoding its transmembrane region. The human homologue of neu, c-erbB-2, can be activated in vitro to an oncogenic form by point mutations in the same relative position in the gene. We have sought the presence of such activating mutations in a series of 100 cases of human breast cancer by polymerase chain reaction amplification and sequence-specific oligonucleotide hybridization, and also by a designed restriction fragment length polymorphism strategy in cases with Southern blot evidence of c-erbB-2 amplification. No evidence of activating point mutations in the c-erbB-2 protooncogene was found in any of these cases.
Lemoine
N R
NR
ICRF Molecular Oncology Group, Hammersmith Hospital, Road, London.
Staddon
S
S
Dickson
C
C
Barnes
D M
DM
Gullick
W J
WJ
D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics*; D066246:ErbB Receptors; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D011519:Proto-Oncogenes
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D066246:ErbB Receptors