Alterations in acetylcholine-induced stimulation of inositol phospholipid hydrolysis in the dorsal hippocampus of kindled rats.
Agonist-induced turnover or release of inositolphosphates (IP) was studied in the dorsal and the ventral hippocampus 24 h, 1 month, and 3 months after the last electrical stimulus of kindled rats. A significant increase in acetylcholine (ACh)-stimulated IP turnover was observed in dorsal, but not ventral, hippocampus 24 h and 1 month after the last electrical stimulus. However, this effect was not evident 3 months after kindling. The excitatory amino acids (quisqualic acid and ibotenic acid) at the concentrations used, however, failed to produce any change in receptor-stimulated release or turnover of IP. Thus the changes in ACh-induced IP release, although long-term, are not permanent and do not appear to be released to the neurobiological alterations associated with the long-term maintenance of the kindling phenomenon.
Zhang
W Q
WQ
Laboratory of Molecular and Integrative Neuroscience, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.
Tandon
P
P
Sobotka
T J
TJ
Hong
J S
JS
Tilson
H A
HA
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D004558:Electric Stimulation; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D006868:Hydrolysis; D007051:Ibotenic Acid / Q000494:pharmacology; D007696:Kindling, Neurologic / Q000502:physiology*; D008297:Male; D010716:Phosphatidylinositols / Q000378:metabolism*; D016318:Quisqualic Acid / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344; D013238:Stereotaxic Techniques
D010716:Phosphatidylinositols; D007051:Ibotenic Acid; D016318:Quisqualic Acid; D000109:Acetylcholine