Identification and structural analysis of a ribosomal RNA gene promoter from Thiobacillus ferrooxidans.
The 5'-terminus of a rRNA operon (rrnT2) from Thiobacillus ferrooxidans was characterized. The rRNA promoters from this microorganism were identified by means of a functional assay in Escherichia coli. DNA sequencing of the promoter region, upstream the 16 S rRNA gene, showed the presence of a consensus sequence for bacterial ribosomal promoters. Other features such as a 'discriminator' sequence, antiterminator elements and an upstream hexanucleotide common to several rRNA operons were also found. Two other putative transcription promoters were also identified.
Takamiya
M
M
Departamento de Bioquimica, Facultad de Medicina, Universidad de Chile, Santiago.
Salazar
O
O
Vargas
D
D
Jedlicki
E
E
Orellana
O
O
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009876:Operon; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012335:RNA, Ribosomal / Q000737:chemistry / Q000235:genetics*; D013855:Thiobacillus / Q000235:genetics*; D014158:Transcription, Genetic; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D012335:RNA, Ribosomal; D004262:DNA Restriction Enzymes