Inhibition of herpes virus plaquing capacity in human diploid fibroblasts treated with gilvocarcin V plus near UV radiation.
The capacity of human fibroblasts to support plaque formation by Herpes simplex virus following treatment of the cells with gilvocarcin V, a polyaromatic C-glycoside, plus near ultraviolet radiation (UVA, 320-400 nm) was examined. Gilvocarcin V, plus UVA radiation, effectively inhibited host cell capacity at concentrations five orders of magnitude lower than that of 8-methyoxypsoralen required for capacity inhibition at similar levels of UVA radiation. This result extends the observation of unusual biological potency of UVA-activated gilvocarcins from bacterial cells to human cells.
Bockstahler
L E
LE
Office of Science and Technology, Center for Devices and Radiological Health, Food and Drug Administration, Rockville, MD 20857.
Elespuru
R K
RK
Hitchins
V M
VM
Carney
P G
PG
Olvey
K M
KM
Lytle
C D
CD
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D003374:Coumarins; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects; D006027:Glycosides; D006801:Humans; D008730:Methoxsalen / Q000494:pharmacology; D018139:Simplexvirus / Q000187:drug effects / Q000254:growth & development / Q000528:radiation effects*; D012867:Skin / Q000187:drug effects / Q000528:radiation effects; D010948:Viral Plaque Assay
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000998:Antiviral Agents; D003374:Coumarins; D006027:Glycosides; C031781:gilvocarcin V; D008730:Methoxsalen