IS30 activation of an smp'-lacZ gene fusion in Escherichia coli.
The Escherichia coli serB gene is divergently transcribed from the gene. Six independently isolated IS30 insertions within the 5' end of the serB structural gene resulted in increased expression of an smp'-lacZ gene fusion. DNA sequence analysis of two of the insertions suggests that a promoter was created upon IS30 insertion.
Neuwald
A F
AF
Department of Microbiology, University of Iowa, Iowa City 52242.
Stauffer
G V
GV
D001483:Base Sequence; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D007763:Lac Operon / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D011401:Promoter Regions, Genetic
D004251:DNA Transposable Elements