Elevated gelsolin and alpha-actin expression in a flat revertant R1 of Ha-ras oncogene-transformed NIH/3T3 cells.
Expressions of gelsolin and alpha-actin have been investigated in a revertant cell line R1 and compared with the parental human activated Ha-ras oncogene-transformed NIH/3T3 (EJ-NIH/3T3), untransformed NIH/3T3 and partially revertant R2 cells. Gelsolin mRNA expression was strongest in R1 cells, intermediate in R2 and NIH/3T3 cells, and low in EJ-NIH/3T3 cells. Southern blot analysis gave neither signs of gross rearrangements nor amplification of the gelsolin gene. alpha-actin mRNA expression was restored in R1 cells to the level of NIH/3T3 cells. In R2 and EJ-NIH/3T3 cell lines, no alpha-actin transcript was detected. High gelsolin expression and restoration of alpha-actin expression may be associated with the acquirement of flat morphology and ordered cell growth pattern, which imply loss of tumorigenicity of R1 cells.
Müllauer
L
L
Laboratory of Molecular Genetics, Cancer Institute, Hokkaido University School of Medicine, Sapporo, Japan.
Fujita
H
H
Suzuki
H
H
Katabami
M
M
Hitomi
Y
Y
Ogiso
Y
Y
Kuzumaki
N
N
D000199:Actins / Q000032:analysis / Q000235:genetics*; D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000235:genetics*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D005455:Fluorescent Antibody Technique; D018260:Gelsolin; D011905:Genes, ras; D006801:Humans; D051379:Mice; D008840:Microfilament Proteins / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping
D000199:Actins; D002135:Calcium-Binding Proteins; D018260:Gelsolin; D008840:Microfilament Proteins; D012333:RNA, Messenger