Expression and secretion of preS containing hepatitis B surface antigen in vaccinia virus system.
The expression and secretion of preS containing hepatitis B surface antigen in vaccinia virus system was investigated. The human TK- 143 cells were infected with the recombinant vaccinia viruses vTMS-1 or vTLS-1. Cells infected with vTMS-1, which contains the preS2 + S gene, produced preS2 containing middle HBsAg proteins. Similarly, cells produced preS1 containing large HBsAg proteins upon infection with vTLS-1, which carries the preS1 + preS2 + S gene. The expression products could be secreted and form 22 nm particles. They reacted specifically with anti-preS1 and/or anti-preS2 monoclonal antibodies, and exhibited pHSA-receptor (for polymerized human serum albumin) activity. In addition, the major S components of hepatitis B surface antigen were also present in the products expressed by vTMS-1 and vTLS-1.
Wang
Y A
YA
Shanghai Institute of Biochemistry, Academia Sinica, Shanghai, PRC.
Wang
S Q
SQ
Feng
Z M
ZM
Zhong
W W
WW
Kong
Y Y
YY
Li
Z P
ZP
Lu
X Y
XY
Yao
G B
GB
D002478:Cells, Cultured; D004274:DNA, Recombinant; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D011498:Protein Precursors / Q000032:analysis*; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface / Q000378:metabolism; D014616:Vaccinia virus / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000032:analysis
D004274:DNA, Recombinant; D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; D017457:Receptors, Albumin; D011956:Receptors, Cell Surface; D014759:Viral Envelope Proteins; C050806:presurface protein 1, hepatitis B surface antigen; C050807:presurface protein 2, hepatitis B surface antigen