[Use of a rapid method for detecting adenovirus in the feces of children].
Adenovirus (ADV) and rotavirus (RV) were screened by latex aglutination (LA) in 772 fecal samples from hospitalized and non hospitalized children under 7 years of age, those included 55 control samples. Reactive samples were confirmed by electron microscopy (ME) and enzimoimmunoassay (EIA). ADV were found in 20 (2.6%) samples and RV in 25 (3.2%). Coinfection by both viruses was not demonstrated. The greatest percentage of findings was observed in watery (23% ADV and 25% RV) and almost watery stools (8% ADV and 25% RV). We did not find any of those viruses in samples from control children. Concordance between LA and confirmation methods used was excellent. Thus the LA tests could provide a simple tool for the diagnosis of non hospitalized patients, increasing the possibilities of a rapid ethiological diagnostic in infectious diarrhoeas.
Ortiz de Lejarazu Leonardo
R
R
Facultad de Medicina de Valladolid.
Reguera Useros
J I
JI
Coca García
M C
MC
Alonso Franch
M
M
Machín Acosta
M P
MP
López Mongil
M R
MR
Eirós Bouza
J M
JM
Rodríguez Torres
A
A
D000256:Adenoviridae / Q000302:isolation & purification*; D000293:Adolescent; D002675:Child, Preschool; D003968:Diarrhea, Infantile / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D012401:Rotavirus / Q000302:isolation & purification
Utilidad de una técnica rápida para detección de adenovirus en heces de niños.