title: Amino acid receptors from insect muscle: electrophysiological characterization in Xenopus oocytes following expression by injection of mRNA.
abstract: Poly(A)+ Messenger ribonucleic acid (mRNA) was extracted from leg muscles of the locust Schistocerca gregaria and injected into oocytes of Xenopus laevis. After 5-10 days incubation, receptors for L-glutamate, L-quisqualate, DL-ibotenate and gamma-aminobutyric acid (GABA) were expressed. Agonist-induced currents were dose-dependent, and, in the concentration range 1 microM to 1 mM, generally had peak values of 50 nA. The responses to all agonists, apart from GABA, exhibited desensitization which could not be reversed even by prolonged washing with Ringer. Application of 100 microM GABA to oocytes voltage clamped at -60 mV produced a smooth inward current with a reversal potential of -22 +/- 1 mV, which is consistent with the involvement of chloride ions. At 100 microM, picrotoxin reversibly abolished this current, while 100 microM bicuculline had no effect. L-Glutamate elicited a smooth current with a reversal potential of -52 +/- 3 mV. L-Quisqualate elicited an inward current at -60 mV with a reversal potential of -9 +/- 2 mV; this current occasionally had an oscillatory component. The response to ibotenate comprised a smooth inward current with a reversal potential of -21 +/- 3 mV which was probably mediated by chloride ions.
authors.affiliation: Imperial College of Science, Technology and Medicine, Department of Biology, London, U.K.
authors.forename: S P
authors.initials: SP
authors.lastname: Fraser
authors.forename: M B
authors.initials: MB
authors.lastname: Djamgoz
authors.forename: P N
authors.initials: PN
authors.lastname: Usherwood
authors.forename: J
authors.initials: J
authors.lastname: O'Brien
authors.forename: M G
authors.initials: MG
authors.lastname: Darlison
authors.forename: E A
authors.initials: EA
authors.lastname: Barnard
mesh_terms: D000596:Amino Acids; D000818:Animals; D004594:Electrophysiology; D005260:Female; D005971:Glutamates / Q000494:pharmacology; D006110:Grasshoppers / Q000378:metabolism*; D008845:Microinjections; D009132:Muscles / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism*; D010852:Picrotoxin / Q000494:pharmacology; D011061:Poly A / Q000302:isolation & purification; D016318:Quisqualic Acid / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D014982:Xenopus laevis; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D005971:Glutamates; D010852:Picrotoxin; D011061:Poly A; D016318:Quisqualic Acid; D012333:RNA, Messenger; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface; D005680:gamma-Aminobutyric Acid