title: Ontogeny of plasma, CSF and brainstem ACTH in piglets: effects of hypoxia and anesthesia.
abstract: This study was designed to assess the postnatal maturation of adrenocorticotropin (ACTH) levels in piglets under basal conditions and in response to single, acute stressors. ACTH levels were measured by radioimmunoassay in plasma, cerebrospinal fluid (CSF) and in a dorsal medullary slice containing the nucleus tractus solitarii (dmscNTS) of young and older piglets (1.5-6 and 35-43 days old, respectively) under the following experimental conditions: (1) normoxia (both groups); (2) hypoxia, 10% O2/N2 for greater than or equal to 30 min (both groups); (3) sham anesthesia, i.p. saline in normoxia (young group); (4) anesthesia, 25 mg/kg i.p. pentobarbital in normoxia (young group), and (5) anesthesia combined with hypoxia (young group). During normoxia, ACTH levels in young, as compared to older piglets, were higher in CSF (p less than 0.01) and plasma (0.05 less than p less than 0.10) and not different in dmscNTS. Hypoxia produced no ACTH changes in CSF, increased ACTH in plasma of young (p = 0.03) and older piglets (p = 0.09), and decreased ACTH in dmscNTS of older (p = 0.01) and young piglets (p = 0.07). As compared to sham anesthesia, anesthesia did not alter any ACTH levels. Combined hypoxia and anesthesia increased ACTH levels in plasma when compared to normoxia (p less than 0.05), sham anesthesia (p less than 0.05) or anesthesia alone (p less than 0.05), but not when compared to hypoxia alone. We conclude that neonatal swine have high basal ACTH levels and mount significant plasma ACTH responses to a single, acute hypoxic stressor. The presence of ACTH in the region of the NTS supports its possible role as a neuromodulator in the brain.
authors.affiliation: Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas.
authors.forename: I R
authors.initials: IR
authors.lastname: Moss
authors.forename: J G
authors.initials: JG
authors.lastname: Inman
authors.forename: J C
authors.initials: JC
authors.lastname: Porter
authors.forename: D J
authors.initials: DJ
authors.lastname: Faucher
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism*; D000758:Anesthesia; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D001933:Brain Stem / Q000187:drug effects / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology*; D013552:Swine / Q000254:growth & development; D001615:beta-Endorphin / Q000378:metabolism
chemical_list: D001615:beta-Endorphin; D000324:Adrenocorticotropic Hormone; D010100:Oxygen