Alpha-adrenoceptor activation of polyphosphoinositide hydrolysis in the rat tail artery.
alpha-Adrenoceptor coupling to polyphosphoinositide (PI) hydrolysis was studied in the rat tail artery. Inositol phosphate (IP) accumulation was stimulated by the non-selective alpha-adrenoceptor agonist norepinephrine and the alpha 1-adrenoceptor agonist phenylephrine. This stimulation was relatively dependent on extracellular Ca2+ and enhanced markedly in the presence of LiCl. In addition, norepinephrine- and phenylephrine-stimulated IP accumulation was relatively sensitive to blockade by prazosin, compared to rauwolscine. The putative alpha 2-adrenoceptor agonist UK 14304 also stimulated PI breakdown in a concentration-dependent manner, although this stimulation did not reach equilibrium at up to 10 mM and was relatively sensitive to prazosin, compared to rauwolscine, over the lower agonist concentrations. NaF stimulated IP accumulation independently of alpha-adrenoceptor activation. PI breakdown by alpha-adrenoceptor agonists and NaF was attenuated by N-ethylmaleimide but not pertussis toxin treatment. In addition, dithiothreitol blocked NaF-stimulated, but not alpha-adrenoceptor-mediated. PI breakdown. These results suggest the coupling of alpha 1-adrenoceptor, via phospholipase C, to PI hydrolysis in the rat tail artery. This study also provides evidence for the involvement of one or more non-Gi-like G-protein(s) in the signal transduction process.
Cheung
Y D
YD
Division of Basic Medical Sciences, Faculty of Medicine, Memorial University of Newfoundland, St. John's, Canada.
Feltham
I
I
Thompson
P
P
Triggle
C R
CR
D000818:Animals; D001158:Arteries / Q000378:metabolism; D002118:Calcium / Q000502:physiology; D004229:Dithiothreitol / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D005260:Female; D019204:GTP-Binding Proteins / Q000502:physiology; D006868:Hydrolysis; D008094:Lithium / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D037342:Pertussis Toxin; D010716:Phosphatidylinositols / Q000378:metabolism*; D051381:Rats; D011942:Receptors, Adrenergic, alpha / Q000502:physiology*; D012969:Sodium Fluoride / Q000494:pharmacology; D013623:Tail / Q000098:blood supply; D010738:Type C Phospholipases / Q000502:physiology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D010716:Phosphatidylinositols; D011942:Receptors, Adrenergic, alpha; D010566:Virulence Factors, Bordetella; D012969:Sodium Fluoride; D008094:Lithium; D037342:Pertussis Toxin; D010738:Type C Phospholipases; D019204:GTP-Binding Proteins; D005033:Ethylmaleimide; D002118:Calcium; D004229:Dithiothreitol