Autoantibodies to topoisomerase I (Scl-70): analysis by gel diffusion, immunoblot, and enzyme-linked immunosorbent assay.
Anti-topoisomerase I autoantibodies (anti-topo I, anti-Scl-70) are associated with proximal scleroderma and are of prognostic significance in patients with Raynaud's disease. To establish a highly sensitive and specific system for the detection of anti-topo I, we have investigated sera from 409 patients and controls by Ouchterlony gel diffusion, Western immunoblot on chromosome proteins, and solid-phase enzyme-linked immunosorbent assay (ELISA) with purified topoisomerase I as antigen. The ELISA was more sensitive than the gel diffusion technique and was more specific than the Western immunoblot, while the immunoblot may identify additional autoantibodies.
Hildebrandt
S
S
Department of Medicine, University of Connecticut School of Medicine, Farmington 06032.
Weiner
E S
ES
Senecal
J L
JL
Noell
G S
GS
Earnshaw
W C
WC
Rothfield
N F
NF
D001323:Autoantibodies / Q000032:analysis*; D001324:Autoantigens / Q000032:analysis* / Q000276:immunology; D015153:Blotting, Western; D004264:DNA Topoisomerases, Type I / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D005779:Immunodiffusion; D008297:Male; D009687:Nuclear Proteins / Q000032:analysis* / Q000276:immunology; D011928:Raynaud Disease / Q000175:diagnosis / Q000276:immunology; D012595:Scleroderma, Systemic / Q000175:diagnosis / Q000276:immunology
D001323:Autoantibodies; D001324:Autoantigens; D009687:Nuclear Proteins; C048100:Scl 70 antigen, human; D004264:DNA Topoisomerases, Type I