Cloning, localization, and permanent expression of a Drosophila octopamine receptor.
A cDNA for a member of the G protein-coupled receptor family was isolated from Drosophila using a probe derived from a human beta 2-adrenergic receptor cDNA. This Drosophila receptor gene is localized at 99A10-B1 on the right arm of chromosome 3 and is preferentially expressed in Drosophila heads. The insect octopamine receptor has been permanently expressed in mammalian cells, where it mediates the attenuation of adenylate cyclase activity and exhibits a pharmacological profile consistent with an octopamine type 1 receptor. Sequence and pharmacological comparisons indicate that the octopamine receptor is unique but closely related to mammalian adrenergic receptors, perhaps as an evolutionary precursor.
Arakawa
S
S
Section of Receptor Biochemistry and Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892.
Gocayne
J D
JD
McCombie
W R
WR
Urquhart
D A
DA
Hall
L M
LM
Fraser
C M
CM
Venter
J C
JC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002460:Cell Line; D002874:Chromosome Mapping; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004330:Drosophila / Q000235:genetics*; D019204:GTP-Binding Proteins / Q000235:genetics; D015870:Gene Expression; D005796:Genes; D015698:Genomic Library; D006801:Humans; D008969:Molecular Sequence Data; D009655:Octopamine / Q000378:metabolism; D015345:Oligonucleotide Probes; D011487:Protein Conformation; D011941:Receptors, Adrenergic / Q000235:genetics*; D011943:Receptors, Adrenergic, beta / Q000235:genetics; D017982:Receptors, Biogenic Amine; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D015345:Oligonucleotide Probes; D011941:Receptors, Adrenergic; D011943:Receptors, Adrenergic, beta; D017982:Receptors, Biogenic Amine; C027604:norsynephrine receptor; D009655:Octopamine; D004247:DNA; D019204:GTP-Binding Proteins