Overproduction of superoxide dismutase does not protect Escherichia coli from stringency-induced growth inhibition by 1mM paraquat.
Two plasmid-containing Escherichia coli strains which overproduce manganese superoxide dismutase by 4- to 5-fold and iron superoxide dismutase by about 7-fold were not more resistant than parent strains to 1 mM paraquat (a known generator of superoxide) as measured by effects on growth, survival and induction of stringency. These results indicate that overproduction of superoxide dismutase does not mitigate the growth-inhibitory effects of 1 mM paraquat, including those which are expressed through induction of the stringency mechanism.
Siwecki
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G
Department of Microbiology, School of Medicine, University of Missouri, Columbia 65211.
Brown
O R
OR
D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D006159:Guanosine Tetraphosphate / Q000032:analysis; D010269:Paraquat / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000378:metabolism*; D013997:Time Factors
D006159:Guanosine Tetraphosphate; D013482:Superoxide Dismutase; D010269:Paraquat