Latex test for rapid rotavirus diagnosis in calves.
A latex agglutination test (LA) was compared with direct electron microscopy (EM) for detection of rotavirus infection in calves. A total of 375 samples from 62 calves were collected. Samples were taken when the calves were 1, 3, 5, 7, 10 and 20 days old and some scours samples were collected as well. Altogether 45/375 (12%) specimens were positive in LA and 10/375 (2.7%) were positive in EM. Samples positive in EM were also positive in LA. Out of the 62 calves studied 26/62 (42%) were positive in LA and 8/62 (13%) in EM. We found the LA very easy to perform, to be more sensitive than the EM method and probably a rather specific method for detection of rotavirus infection.
Sukura
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Department of Anatomy and Embryology, College of Veterinary Medicine, National Veterinary Institute, Helsinki, Finland.
Neuvonen
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E
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D003967:Diarrhea / Q000175:diagnosis / Q000662:veterinary*; D005243:Feces / Q000382:microbiology; D007841:Latex Fixation Tests; D008854:Microscopy, Electron; D012401:Rotavirus / Q000648:ultrastructure; D012400:Rotavirus Infections / Q000175:diagnosis / Q000662:veterinary*