The OGD1 gene, affecting 2-oxoglutarate dehydrogenase in S. cerevisiae, is closely linked to HIS5 on chromosome IX.
Ogd1 mutants of Saccharomyces cerevisiae are deficient in mitochondrial 2-oxoglutarate dehydrogenase activity; they cannot grow on glycerol and produce an increased amount of organic acids during growth on glucose as substrate. Using gamma ray-induced rad52-mediated chromosome loss the ogd1 mutation can be assigned to chromosome IX. Tetrad analysis of crosses between ogd1 and other markers on chromosome IX revealed that the OGD1 gene maps on the left arm of this chromosome 1.9 cM from his5.
Ruttkay-Nedecký
B
B
Food Research Institute, Bratislava, Czechoslovakia.
Subík
J
J
D002874:Chromosome Mapping; D015825:Chromosomes, Fungal / Q000528:radiation effects; D005720:Gamma Rays; D005800:Genes, Fungal; D008040:Genetic Linkage; D005838:Genotype; D007655:Ketoglutarate Dehydrogenase Complex / Q000235:genetics*; D007658:Ketone Oxidoreductases / Q000235:genetics*; D008928:Mitochondria / Q000201:enzymology; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development
D007658:Ketone Oxidoreductases; D007655:Ketoglutarate Dehydrogenase Complex