3'-5' cAMP phosphodiesterase in pig liver nuclei.
cAMP phosphodiesterase was partially purified from pig liver nuclei and some of its properties were investigated. The enzyme is a low km PDE. Inhibition by xanthine derivatives is in line with the behaviour of PDE from other mammalian sources, while small differences are observed towards nucleotides inhibitors. The enzyme is a chromatin-bound protein although no specific location related to transcriptional function could be demonstrated.
Lupidi
G
G
Dipartimento di Biologia Cellulare, Università di Camerino.
Eufemi
M
M
Luciani
S
S
Ferraro
A
A
Riva
F
F
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000201:enzymology*; D002843:Chromatin / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology; D013045:Species Specificity; D013552:Swine
D002843:Chromatin; D015105:3',5'-Cyclic-AMP Phosphodiesterases