Identification of the gamma 2-subunit protein in native GABAA receptors in brain.
GABAA receptors with functional benzodiazepine receptors have been reconstituted by coexpression of alpha-, beta- and gamma-subunit cDNAs. In brain, proteins of the alpha- and beta-subunits, but not the gamma 2-subunit have been identified. Using an antipeptide antiserum, we now demonstrate that the gamma 2-subunit is a protein of 43 kDa. It is present in at least 50% of GABAA receptors, as shown by immunoprecipitation.
Benke
D
D
Institute of Pharmacology, University of Zurich, Switzerland.
Mertens
S
S
Trezciak
A
A
Gillessen
D
D
Mohler
H
H
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D001923:Brain Chemistry; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000276:immunology; D011233:Precipitin Tests; D051381:Rats; D011963:Receptors, GABA-A / Q000737:chemistry* / Q000276:immunology
D009419:Nerve Tissue Proteins; D011963:Receptors, GABA-A