title: The gdhA gene is located at 38.6 minutes on the Escherichia coli map.
abstract: We report here that the gdhA gene of Escherichia coli, which encodes the NADP-specific glutamate dehydrogenase, is located at 38.6 min on the map. We have confirmed this location by showing linkage with three Tn10 insertions that are linked to the aroD, pheS, and ansA loci, by complementation by a restriction-mapped lambda clone, and by showing correspondence between the restriction maps of the chromosome and the cloned and sequenced gdhA gene.
authors.affiliation: Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
authors.forename: S Y
authors.initials: SY
authors.lastname: Kim
authors.forename: D
authors.initials: D
authors.lastname: McLaggan
authors.forename: W
authors.initials: W
authors.lastname: Epstein
mesh_terms: D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005816:Genetic Complementation Test; D008040:Genetic Linkage; D005969:Glutamate Dehydrogenase / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D004251:DNA Transposable Elements; D005969:Glutamate Dehydrogenase