Inhibition of peptidoglycan biosynthesis in Bacillus megaterium by daptomycin.
The effects of daptomycin on exponential phase cells of Bacillus megaterium were investigated. Bacteriostasis was observed for concentrations between 1 and 3 micrograms/ml and maximal rate of cell lysis at 10 micrograms/ml. At sublytic concentrations (1.5-3 micrograms/ml), the variations of the pools of UDP-N-acetylglucosamine and UDP-N-acetylmuramyl-pentapeptide, as well as the incorporation of (14C)-N-acetylglucosamine into peptidoglycan were studied. From the results it was concluded that the lethal target of daptomycin could be a metabolic step between glucosamine 6-phosphate and UDP-N-acetylglucosamine.
Mengin-Lecreulx
D
D
Unité de Recherche Associée du CNRS, URA 1131, Biochimie Moléculaire et Cellulaire, Université Paris-Sud, Orsay, France.
Allen
N E
NE
Hobbs
J N
JN
van Heijenoort
J
J
D000117:Acetylglucosamine / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001410:Bacillus megaterium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D017576:Daptomycin; D010455:Peptides / Q000494:pharmacology; D010457:Peptidoglycan / Q000096:biosynthesis*
D000900:Anti-Bacterial Agents; D010455:Peptides; D010457:Peptidoglycan; D017576:Daptomycin; D000117:Acetylglucosamine