Possible role of H1 histone in the differentiation of mouse erythroleukemia cells.
Serum-starved mouse erythroleukemia cells, stationary phase cells or cells cultured in dibutyryl cAMP (1 mM) can be induced to differentiate by addition of 20% fetal calf serum plus cycloheximide. Culturing unstarved log phase cells in 20% fetal calf serum plus low levels of cycloheximide and histone H1 also causes a significant level of differentiation. These same concentrations of cycloheximide and H1 histone employed separately with 20% fetal calf serum do not induce differentiation. The role these procedures may have in causing an accumulation of histone H1 and cell differentiation is discussed.
Flickinger
R A
RA
Department of Biological Sciences, State University of New York, Buffalo 14260.
Brooks
E
E
Choi
P
P
Morlock
M
M
Parker
B
B
Snider
J
J
D000818:Animals; D001769:Blood; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D006657:Histones / Q000378:metabolism*; D004915:Leukemia, Erythroblastic, Acute; D051379:Mice; D014407:Tumor Cells, Cultured
D003470:Culture Media; D006657:Histones; D003994:Bucladesine; D003513:Cycloheximide