[Production and characterization of hybrid monoclonal antibodies with IgG1/IgG3 double isotype].
In order to obtain bispecific monoclonal antibodies, two hybridoma cell lines were fused: E13 producing an IgG1 anti-Cytomegalovirus, and 70B12 producing an IgG3 anti-Chlamydia. This experimental model was chosen to test the possibility of association between gamma 1 gamma 3 heavy chains. This association was shown by using an ELISA.
Lebègue
V
V
D.I.C.A. Division d'Immuno-Cytologie appliquée, Université de Technologie de Compiègne.
Joron
L
L
Barque
J P
JP
Pancino
G
G
Roseto
A
A
D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D002459:Cell Fusion; D002689:Chlamydia / Q000276:immunology; D002999:Clone Cells; D003587:Cytomegalovirus / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006822:Hybrid Cells / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D007132:Immunoglobulin Isotypes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes
Production et caractérisation d'anticorps monoclonaux hybrides présentant une double isotypie IgG1/IgG3.