Infection of CV1 cells expressing the polyoma virus middle T antigen or the SV40 agnogene product with simian virus 40 host-range mutants.
SV40 viruses bearing mutations at the carboxy-terminus of large T antigen exhibit a host-range phenotype: such viruses are able to grow in BSC monkey kidney cells at 37 degrees C, but give at least 10,000-fold lower yields than wild type virus in BSC cells at 32 degrees C or in CV1 monkey kidney cells at either temperature. The block to infection in the nonpermissive cell type occurs after the onset of viral DNA replication. Infectious progeny virions are produced at very low efficiency. Although capsid proteins are synthesized at decreased levels, this does not account for the magnitude of the defect. Presumably some step of virion assembly or maturation is affected in these mutants. We have previously reported that the viral agnogene product, a protein thought to be involved in viral assembly or release, fails to accumulate in CV1 cells infected with host-range mutants. In polyoma virus the middle T antigen plays a role in virion maturation by influencing the phosphorylation of capsid proteins. In this communication we show that host-range mutants fail to undergo productive infection of CV1 cells expressing middle T antigen. These mutants do form plaques on an agnoprotein-expressing cell line. However, the agnoprotein does not seem to act by correcting the mutational block but rather increases the efficiency of plaque formation.
Spence
S L
SL
Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260.
Tack
L C
LC
Wright
J H
JH
Carswell
S
S
Pipas
J M
JM
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D002213:Capsid / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D002460:Cell Line; D002472:Cell Transformation, Viral; D015870:Gene Expression; D005814:Genes, Viral; D007700:Kinetics; D009154:Mutation; D010641:Phenotype; D010766:Phosphorylation; D013539:Simian virus 40 / Q000235:genetics* / Q000276:immunology; D013696:Temperature; D014157:Transcription Factors / Q000235:genetics; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics*; D054334:Viral Regulatory and Accessory Proteins; D000086082:Viroporin Proteins
D000952:Antigens, Polyomavirus Transforming; D014157:Transcription Factors; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C042559:agnoprotein, polyomavirus; D000086082:Viroporin Proteins