Modulation of cardiac beta-adrenergic receptors by dopamine beta-hydroxylase.
Incubation of cardiac sarcolemma in the presence of dopamine beta-hydroxylase (DBH), a catecholamine biosynthetic enzyme, increased beta-adrenergic receptor density by 68% as measured by [3H]dihydroalprenolol (DHA) binding. The addition of DBH to plasma membranes isolated from brain, kidney, skeletal muscle, liver and intestine did not alter [3H]DHA binding. Cardiac alpha-receptors were unaffected under similar conditions. Since DBH is coreleased with norepinephrine, these results indicate that a functional coupling of the putative beta-adrenergic receptor with DBH may exist in cardiac muscle.
Ganguly
P K
PK
Department of Anatomy, St. Boniface General Hospital Research Centre, Winnipeg, Canada.
Lee
S L
SL
Waghray
G
G
D000818:Animals; D004082:Dihydroalprenolol / Q000378:metabolism; D004299:Dopamine beta-Hydroxylase / Q000494:pharmacology*; D006352:Heart Ventricles / Q000378:metabolism; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D007700:Kinetics; D009200:Myocardial Contraction / Q000187:drug effects*; D009206:Myocardium / Q000378:metabolism*; D009928:Organ Specificity; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000378:metabolism*; D012508:Sarcolemma / Q000378:metabolism*
D011943:Receptors, Adrenergic, beta; D004082:Dihydroalprenolol; D004299:Dopamine beta-Hydroxylase; D007545:Isoproterenol