Expression of retinoic acid receptor genes and the ligand-binding selectivity of retinoic acid receptors (RAR's).
Retinoids of a structurally new type, 4-(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-naphthalenylcarbamoyl)benzoic acid (Am80) and (E)-4-[3-(3,5-di-tert-butylphenyl)-3-oxo-1-propenyl]-benzoic acid (Ch55), were used for the investigation of retinoid receptors in some human cell lines. Previously, using these retinoids, we have established that HL-60 cells possess two retinoid receptors, RAR-alpha and RAR-beta. In this paper, we report coexpression of RAR-alpha- and RAR-beta-genes established by Northern analysis. Further, the binding selectivity of the RAR products toward all-trans-retinoic acid (RA), Am80 and Ch55 was studied by filter binding assay. The affinity of the ligands toward RAR-alpha decreased in the order of Ch55 greater than RA greater than Am80. The affinity of the ligands toward RAR-beta decreased in the order of Ch55 greater than Am80 greater than RA.
Hashimoto
Y
Y
Institute of Applied Microbiology, University of Tokyo, Japan.
Kagechika
H
H
Shudo
K
K
D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D015870:Gene Expression; D005796:Genes; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008024:Ligands; D009363:Neoplasm Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D018168:Receptors, Retinoic Acid; D013379:Substrate Specificity; D014158:Transcription, Genetic; D014162:Transfection; D014212:Tretinoin / Q000378:metabolism*
D002352:Carrier Proteins; D008024:Ligands; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; D014212:Tretinoin