title: Regional variation of biochemical characteristics and antigeneity in Great Basin rattlesnake (Crotalus viridis lutosus) venom.
abstract: 1. Three pooled and 20 individual venom samples of Crotalus viridis lutosus from different localities in Utah and Arizona were screened and fractionated with HPLC-anion exchange. 2. Pooled venom samples and fractions were tested for hemorrhagic, collagenase, and phospholipase activities, and reactivity to a monoclonal antibody against a hemorrhagin from C. atrox venom (CA-P-8) using ELISA. 3. The 20 individual samples were organized into four groups based on their HPLC profiles. 4. ELISA results and specific hemorrhagic activity of the venom samples displayed a variation in latitidinal distribution although from the same species.
authors.affiliation: Department of Biology, Texas A&I University, Kingsville 78363.
authors.forename: B L
authors.initials: BL
authors.lastname: Adame
authors.forename: J G
authors.initials: JG
authors.lastname: Soto
authors.forename: D J
authors.initials: DJ
authors.lastname: Secraw
authors.forename: J C
authors.initials: JC
authors.lastname: Perez
authors.forename: J L
authors.initials: JL
authors.lastname: Glenn
authors.forename: R C
authors.initials: RC
authors.lastname: Straight
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002851:Chromatography, High Pressure Liquid; D003435:Crotalid Venoms / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D010450:Endopeptidases / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D014644:Genetic Variation; D003012:Microbial Collagenase / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism; D012911:Snakes
chemical_list: D000911:Antibodies, Monoclonal; D003435:Crotalid Venoms; D010740:Phospholipases; D010450:Endopeptidases; C041605:hemorrhagic proteinase IV; D003012:Microbial Collagenase