Association of simian virus 40 small-t antigen with the 61-kilodalton component of a cellular protein complex.
Two cellular proteins, 61 and 37 kDa, are found in association with the simian virus 40 (SV40) small-t antigen. Fractionation in standard chromatography systems showed that these proteins were associated with one another in uninfected cells, suggesting that the small-t antigen may bind the complex as a whole and not each individual protein independently. In the presence of N-ethylmaleimide, the 37-kDA protein was selectively released from immune complexes, leaving the small-t antigen and 61-kDa protein in association. This result suggests that the small-t antigen may bind only the 61-kDa protein and that the 37-kDa protein may be associated with immune complexes by virtue of its association with the 61-kDa cellular protein.
Joshi
B
B
Department of Microbiology-Immunology, Northwestern University, Chicago, Illinois 60611.
Rundell
K
K
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism*; D002460:Cell Line; D005033:Ethylmaleimide / Q000494:pharmacology; D000882:Haplorhini; D046911:Macromolecular Substances; D008970:Molecular Weight; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D011485:Protein Binding
D000952:Antigens, Polyomavirus Transforming; D046911:Macromolecular Substances; D010749:Phosphoprotein Phosphatases; D005033:Ethylmaleimide