Cytochalasin-induced nitroblue tetrazolium dye (NBT) reduction in polymorphonuclear leukocytes.
Cytochalasins induce little cytochrome c reduction in intact polymorphonuclear leukocytes (PMNs). A strong activation of the respiratory burst is induced by cytochalasin B or by the other cytochalasins, when the dye nitro blue tetrazolium (NBT) is used to measure the burst. Cytochalasin B-induced NBT reduction is dependent on calcium, which is mainly derived from intracellular stores. In the presence of NBT an enhanced binding of [3H]cytochalasin B can be observed. The enhanced reduction of NBT by cytochalasin B-treated cells is completely inhibited by pretreatment of the PMNs with pertussis toxin, suggesting the involvement of a pertussis toxin-sensitive G-protein in cytochalasin-induced activation of the respiratory burst.
Elferink
J G
JG
Department of Medical Biochemistry, University of Leiden, The Netherlands.
de Koster
B M
BM
Boonen
G J
GJ
D000818:Animals; D003571:Cytochalasin B / Q000494:pharmacology; D003572:Cytochalasins / Q000494:pharmacology*; D003574:Cytochrome c Group / Q000378:metabolism; D066298:In Vitro Techniques; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D009580:Nitroblue Tetrazolium / Q000378:metabolism*; D010084:Oxidation-Reduction; D037342:Pertussis Toxin; D011817:Rabbits; D013778:Tetrazolium Salts / Q000378:metabolism*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D003572:Cytochalasins; D003574:Cytochrome c Group; D013778:Tetrazolium Salts; D010566:Virulence Factors, Bordetella; C031218:cytochalasin A; D009580:Nitroblue Tetrazolium; D003571:Cytochalasin B; D037342:Pertussis Toxin