pH 2 instability of a mutant of mengovirus is related to its interferon sensitivity.
The is-1 mutant of mengovirus is 100 times more sensitive to interferon (IFN) than wild type, as measured by a yield reduction assay in the G3 line of mouse L cells, and is also much more readily inactivated at pH 2. Neither isolated nor encapsidated RNA is degraded under these conditions, which suggests that the pH-sensitive region resides on the virus coat. One-third of the viruses selected for resistance to low pH also showed enhanced resistance to IFN. Attempts to isolate IFN resistant strains directly from is-1 stocks were unsuccessful. These results suggest that either a capsid protein or its precursor is an active anti-IFN agent.
Bakich
E A
EA
Department of Biochemistry and Biophysics, Texas A & M University, College Station.
Monahan
S J
SJ
Simon
E H
EH
D015180:Electrophoresis, Gel, Two-Dimensional; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007370:Interferon Type I / Q000494:pharmacology*; D008574:Mengovirus / Q000187:drug effects / Q000235:genetics*; D009154:Mutation; D012367:RNA, Viral / Q000302:isolation & purification; D013462:Sulfur Radioisotopes; D015678:Viral Structural Proteins / Q000737:chemistry
D007370:Interferon Type I; D012367:RNA, Viral; D013462:Sulfur Radioisotopes; D015678:Viral Structural Proteins