Evaluation of countermeasures for reduction of mouse airborne allergens.
Three kinds of countermeasures for reduction of mouse airborne allergens were evaluated with use of an air sampler and immunochemical methods. Mouse cages and the sampler were placed inside a flexible-film isolator, and concentrations of mouse major allergens in the air were measured. The levels of the airborne allergens, prealbumin and albumin, generated by 10 males, were 3,050 and 492 pg/m3, respectively. Those by 10 females were lower, 317 and 270 pg/m3, respectively. When mouse cages were covered with a filter cap, the airborne allergens inside the isolator were decreased by 90%. When corncob was used as bedding in place of wood shavings, the airborne allergens were decreased by 57 and 77%, respectively. Therefore, for reduction of mouse airborne allergens, we recommend using female mice, covering the cages with filter caps, and using corncob bedding.
Sakaguchi
M
M
Department of Microbiology, Kyorin University, Tokyo, Japan.
Inouye
S
S
Miyazawa
H
H
Kamimura
H
H
Kimura
M
M
Yamazaki
S
S
D000395:Air Pollutants, Occupational / Q000032:analysis*; D000418:Albumins / Q000032:analysis* / Q000302:isolation & purification; D000485:Allergens / Q000032:analysis* / Q000302:isolation & purification; D000818:Animals; D000830:Animals, Laboratory; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D006799:Housing, Animal; D008297:Male; D051379:Mice; D011228:Prealbumin / Q000032:analysis* / Q000302:isolation & purification; D012727:Sex Characteristics
D000395:Air Pollutants, Occupational; D000418:Albumins; D000485:Allergens; D011228:Prealbumin