title: Involvement of pertussis toxin-sensitive GTP-binding protein in prostaglandin F2 alpha- induced phosphoinositide hydrolysis in osteoblast-like cells.
abstract: Prostaglandin F2 alpha (PGF2 alpha) stimulated the formation of inositol phosphates in a dose-dependent manner in cloned osteoblast-like MC3T3-E1 cells. This reaction was markedly inhibited dose-dependently by pertussis toxin. In the cell membranes, pertussis toxin-catalyzed ADP-ribosylation of a 40-kDa protein was significantly attenuated by pretreatment of PGF2 alpha. These results suggest that pertussis toxin-sensitive GTP-binding protein is involved in the coupling of PGF2 alpha receptor to phospholipase C in these cells.
authors.affiliation: First Department of Internal Medicine, Nagoya University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Miwa
authors.forename: H
authors.initials: H
authors.lastname: Tokuda
authors.forename: K
authors.initials: K
authors.lastname: Tsushita
authors.forename: J
authors.initials: J
authors.lastname: Kotoyori
authors.forename: Y
authors.initials: Y
authors.lastname: Takahashi
authors.forename: N
authors.initials: N
authors.lastname: Ozaki
authors.forename: O
authors.initials: O
authors.lastname: Kozawa
authors.forename: Y
authors.initials: Y
authors.lastname: Oiso
mesh_terms: D000818:Animals; D002460:Cell Line; D015237:Dinoprost / Q000494:pharmacology*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006868:Hydrolysis; D007295:Inositol Phosphates / Q000378:metabolism; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism; D010006:Osteoblasts; D037342:Pertussis Toxin; D010716:Phosphatidylinositols / Q000378:metabolism*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
chemical_list: D007295:Inositol Phosphates; D008565:Membrane Proteins; D010716:Phosphatidylinositols; D010566:Virulence Factors, Bordetella; D015237:Dinoprost; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins