Interaction of amosite and surface-modified amosite with a V79-4 (Chinese hamster lung) cell line.
We have been examining a number of chemically modified mineral fibers, derived from amosite asbestos, by in vitro methods to clarify the role of the fiber surface in determining biological activity. The various fibers have identical size distributions but differ in their affinities for components of the cell membrane. They were treated with boiling toluene or chemically modified by treatment with alkyldimethylchlorosilanes (R = C8, C18) that react with free-surface hydroxyl groups to form the corresponding siloxanes. Fibers in MEM supplemented with 15% fetal calf serum were added to a suspension of V79-4 cells labeled with tritiated thymidine and the mixture was incubated. Aliquots of this mixture were spun down on a density gradient to determine the degree of cell-fiber interaction. At 37 degrees C native amosite (UICC standard) stuck to cells within 15 min of incubation, and the amount of sticking was maximum within 70 min. Decreasing the temperature decreased the amount of sticking, and at 20 degrees C no sticking was observable. The chemically modified amosite and the amosite treated with boiling toluene did not stick to the cells even after 70 min. Soaking the toluene-treated amosite with aqueous solutions at room temperature for 48 hr produced a material that had the same sticking properties as the original untreated fiber. These results indicate that the silanol content, and possibly the degree of hydration of the fiber surface, is important for a fiber to stick to a cell surface.
Sara
E A
EA
Medical Research Council Toxicology Unit, Carshalton, Surrey, UK.
Brown
R C
RC
Evans
C E
CE
Hoskins
J A
JA
Simpson
C F
CF
D000818:Animals; D001194:Asbestos / Q000494:pharmacology*; D017639:Asbestos, Amosite; D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D055598:Chemical Phenomena; D002621:Chemistry; D006224:Cricetinae; D003412:Cricetulus; D008168:Lung / Q000166:cytology*; D012825:Silicon / Q000494:pharmacology*; D013499:Surface Properties; D014050:Toluene / Q000494:pharmacology; D014297:Trimethylsilyl Compounds / Q000494:pharmacology*
D014297:Trimethylsilyl Compounds; D017639:Asbestos, Amosite; D001194:Asbestos; D014050:Toluene; D012825:Silicon