title: Transactivation of the Rous sarcoma virus long terminal repeat promoter by Marek's disease virus.
abstract: Transient expression of chloramphenicol acetyltransferase (CAT) was used to study Marek's diseases virus (MDV)-mediated transactivation of the Rous sarcoma virus long terminal repeat (RSV-LTR) promoter. Cotransfection experiments in primary avian cells were conducted using MDV high-molecular-weight DNA and plasmid pRSVcat. Increased CAT activity, relative to controls, was consistently observed in the presence of MDV. Enhanced CAT activity, expressed via the RSV-LTR promoter, was strictly dependent on the presence of MDV DNA or virus, suggesting that activation of the RSV-LTR promoter was due to factors expressed in MDV-infected cells. Differences in transactivation efficiency were observed between various strains and the serotypes of MDV. In particular, high- and low-passage pairs of serotype 1 MDV showed marked differences in their ability to increase CAT activity in pRSVcat-transfected cells. Attenuation of viral pathogenicity and decreased expression of some cell surface glycoproteins occur in high-passage MDV strains. Decreased transactivation ability in these same strains suggests that continuous passage in culture and attenuation may perturb a regulatory mechanism operating by transcriptional control. In addition, transactivation of the RSV-LTR promoter suggests that increased incidence of avian leukosis following vaccination by MDV may be due to MDV-mediated transactivation of endogenous ALV proviral LTR promoters. MDV-mediated transactivation was not limited to the RSV-LTR promoter. Serotype 3 MDV (HVT) efficiently transactivated the herpes simplex virus (HSV) alpha 4 (ICP4) and beta-TK promoters as well as the human cytomegalovirus (hCMV) immediate early promoter.
authors.affiliation: Department of Animal Science, Michigan State University, East Lansing 48824.
authors.forename: V L
authors.initials: VL
authors.lastname: Tieber
authors.forename: L L
authors.initials: LL
authors.lastname: Zalinskis
authors.forename: R F
authors.initials: RF
authors.lastname: Silva
authors.forename: A
authors.initials: A
authors.lastname: Finkelstein
authors.forename: P M
authors.initials: PM
authors.lastname: Coussens
mesh_terms: D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D002460:Cell Line; D002642:Chick Embryo; D003001:Cloning, Molecular; D004372:Ducks; D015967:Gene Expression Regulation, Viral; D008381:Herpesvirus 2, Gallid / Q000145:classification / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012703:Serotyping; D015534:Trans-Activators / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D015534:Trans-Activators; D014764:Viral Proteins