title: Plasmid origin of replication of herpesvirus papio: DNA sequence and enhancer function.
abstract: Herpesvirus papio (HVP) is a lymphotropic virus of baboons which is related to Epstein-Barr virus (EBV) and produces latent infection. The nucleotide sequence of the 5,775-base-pair (bp) EcoRI K fragment of HVP, which has previously been shown to confer the ability to replicate autonomously, has been determined. Within this DNA fragment is a region which bears structural and sequence similarity to the ori-P region of EBV. The HVP ori-P region has a 10- by 26-bp tandem array which is related to the 20- by 30-bp tandem array from the EBV ori-P region. In HVP there is an intervening region of 764 bp followed by five partial copies of the 26-bp monomer. Both the EBV and HVP 3' regions have the potential to form dyad structures which, however, differ in arrangement. We also demonstrate that a transcriptional enhancer which requires transactivation by a virus-encoded factor is present in the HVP ori-P.
authors.affiliation: Department of Microbiology and Immunology, University of North Carolina, Chapel Hill 27599-7295.
authors.forename: D D
authors.initials: DD
authors.lastname: Loeb
authors.forename: N S
authors.initials: NS
authors.lastname: Sung
authors.forename: R L
authors.initials: RL
authors.lastname: Pesano
authors.forename: C J
authors.initials: CJ
authors.lastname: Sexton
authors.forename: C
authors.initials: C
authors.lastname: Hutchison
authors.forename: J S
authors.initials: JS
authors.lastname: Pagano
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004261:DNA Replication; D015246:Deoxyribonuclease EcoRI; D006564:Herpesviridae / Q000235:genetics*; D004854:Herpesvirus 4, Human / Q000235:genetics; D008969:Molecular Sequence Data; D010215:Papio; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D015500:Chloramphenicol O-Acetyltransferase; D015246:Deoxyribonuclease EcoRI