title: Attraction, phasing and neighbour effects of histone octamers on curved DNA.
abstract: Nucleosome core particles were reconstituted on various DNA fragments containing a Crithidia fasciculata kinetoplast curved tract. The results show that, on curved DNA, nucleosome core particles form six- to sevenfold preferentially, relative to bulk sequences. The preferential deposition occurs at multiple periodic positions, whose distribution reveals a unique rotational setting of DNA with respect to the histone octamer surface and whose average periodicity is 10.26 +/- 0.04. Evidence is provided for a context effect in histone octamer deposition: octamers bound to a segment of curved DNA influence the positions of neighbour octamers. Taken together, the preferential formation of nucleosome core particles and the influence on the localization of neighbouring particles suggest for intrinsically bent sequences the biologically relevant role of organizers of nucleosomal arrays.
authors.affiliation: Centro Acidi Nucleici (CNR), Rome, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Costanzo
authors.forename: E
authors.initials: E
authors.lastname: Di Mauro
authors.forename: G
authors.initials: G
authors.lastname: Salina
authors.forename: R
authors.initials: R
authors.lastname: Negri
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D003421:Crithidia / Q000235:genetics / Q000378:metabolism; D004270:DNA, Circular / Q000378:metabolism* / Q000648:ultrastructure; D018105:DNA, Kinetoplast; D005822:Genetic Vectors; D006657:Histones / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000378:metabolism / Q000648:ultrastructure; D011485:Protein Binding; D015183:Restriction Mapping
chemical_list: D004270:DNA, Circular; D018105:DNA, Kinetoplast; D006657:Histones; D009707:Nucleosomes