In vivo ESR measurement of free radicals in whole mice.
The in vivo measurement of nitroxide radicals in whole mouse was carried out by L-band ESR spectroscopy. Spectra were successively observed in hepatic and bladder domains of female mice after intravenous administration of spin-labeled compounds (CPROXYL or TEMPOL). The signal intensities from both domains decreased gradually. The kinetic constants of clearance in the hepatic domain were 0.09/min for CPROXYL and 0.71/min for TEMPOL. The clearance constants in the bladder domain coincided with those in the hepatic domain within experimental error, whereas the constants in collected blood were 1/7-1/10 of those in the hepatic or bladder domains. The mechanism of clearance of nitroxide radicals in whole mice is discussed.
Utsumi
H
H
Department of Health Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Muto
E
E
Masuda
S
S
Hamada
A
A
D000818:Animals; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D005609:Free Radicals; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008657:Metabolic Clearance Rate; D051379:Mice; D009589:Nitrogen Oxides / Q000378:metabolism*; D013113:Spin Labels; D001743:Urinary Bladder / Q000378:metabolism*
D003497:Cyclic N-Oxides; D005609:Free Radicals; D009589:Nitrogen Oxides; D013113:Spin Labels; C047253:3-carbamoyl-2,2,5,5-tetramethyl-3-pyrroline-1-yloxyl; C039900:nitroxyl; C001803:tempol