Dissemination of amikacin resistance gene aphA6 in Acinetobacter spp.
The distribution of the aphA6 gene, encoding a 3'-aminoglycoside phosphotransferase type VI, was studied by dot blot hybridization with 115 amikacin-resistant Acinetobacter strains from various geographical areas. Nucleotide sequences related to aphA6 were found in 109 strains belonging to seven species. As inferred from results of Southern hybridization, dissemination of amikacin resistance in Acinetobacter spp. is due to a gene rather than a strain or plasmid epidemic.
Lambert
T
T
Unité des Agents Antibactériens, Institut Pasteur, Paris, France.
Gerbaud
G
G
Bouvet
P
P
Vieu
J F
JF
Courvalin
P
P
D000150:Acinetobacter / Q000187:drug effects / Q000235:genetics*; D000583:Amikacin / Q000494:pharmacology*; D015139:Blotting, Southern; D003227:Conjugation, Genetic / Q000502:physiology; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial / Q000502:physiology*; D005819:Genetic Markers; D019868:Kanamycin Kinase; D008826:Microbial Sensitivity Tests; D009693:Nucleic Acid Hybridization; D010770:Phosphotransferases / Q000235:genetics; D014170:Transformation, Genetic / Q000502:physiology
D015342:DNA Probes; D004269:DNA, Bacterial; D005819:Genetic Markers; D000583:Amikacin; D010770:Phosphotransferases; D019868:Kanamycin Kinase