The protective effect of normal and pathological (Wilson disease) ceruloplasmins on human erythrocytes.
The binding of the ceruloplasmin (CP) from the healthy donor blood and of ceruloplasmin-like protein (p-CP) isolated from the Wilson patients' blood with erythrocytes (RBC) of healthy donors and with RBC of Wilson's patients (p-RBC) was investigated. It was shown, that the number of CP binding sites both on the RBC and p-RBC was significantly lower than that for p-CP, but Kd value for p-CP binding with both types of erythrocytes was approximately ten times higher than Kd value for CP. The protective action of CP on copper stimulated hemolysis is significantly higher than that of p-CP. The protective action of CP on ferrous ion stimulated hemolysis does not correlate with its ferroxidase activity. Contrariwise, the protective effect of p-CP which has no ferroxidase activity is more powerful than that of CP.
Saenko
E L
EL
A.N. Bach Institute of Biochemistry, USSR Academy of Sciences, Moscow.
Skorobogat'ko
O V
OV
Yaropolov
A I
AI
D002570:Ceruloplasmin / Q000378:metabolism / Q000502:physiology*; D003300:Copper / Q000494:pharmacology; D004912:Erythrocytes / Q000378:metabolism*; D005296:Ferrous Compounds; D006461:Hemolysis / Q000187:drug effects / Q000502:physiology; D006527:Hepatolenticular Degeneration / Q000097:blood*; D006801:Humans; D066298:In Vitro Techniques; D010084:Oxidation-Reduction; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism; D011971:Receptors, Immunologic; D018000:Receptors, Peptide
D005296:Ferrous Compounds; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D018000:Receptors, Peptide; C040519:ceruloplasmin receptor; D003300:Copper; D002570:Ceruloplasmin