Major histocompatibility complex modulation of beta-adrenoceptor function.
Reciprocal interaction between beta-adrenoceptor specific ligand occupancy and alloantibody binding to specific antigens of cardiac and smooth muscle tissues was observed. Interference of alloimmune antibody fixation to both cardiac and oviductal tract preparations by beta 1 or beta 2 selective blockers, respectively, was obtained by means of indirect immunofluorescence assays. Reciprocally, alloimmune IgG and monoclonal antibodies directed to class I H-2 antigens, behaving as beta-adrenoceptor agonists, modified the contractility of both tissues, increasing intracellular levels of cyclic AMP (cAMP). Additionally, alloantibodies were also capable of inhibiting specific beta-adrenoceptor radioligand binding to purified cardiac and smooth muscle membranes. These data suggested a modulation of beta-adrenoceptor function by antibodies directed against H-2 class I histocompatibility molecules, probably through molecular interactions between both structures.
Cremaschi
G
G
Centro de Estudios Farmacológicos y de Principios Naturales, Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina, Buenos Aires.
Borda
E
E
Sales
M
M
Genaro
A
A
Sterin-Borda
L
L
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D002078:Butoxamine / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis; D005260:Female; D006321:Heart / Q000187:drug effects; D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G / Q000494:pharmacology; D008024:Ligands; D008285:Major Histocompatibility Complex / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D009206:Myocardium / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D011217:Practolol / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D014316:Tritium / Q000378:metabolism
D000911:Antibodies, Monoclonal; D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G; D008024:Ligands; D011943:Receptors, Adrenergic, beta; D002078:Butoxamine; D014316:Tritium; D011433:Propranolol; D000242:Cyclic AMP; D011217:Practolol