Effect of polymyxins on the lipopolysaccharide-defective mutants of Proteus mirabilis.
The effects of polymyxins (Pmx) B and E on smooth and rough Proteus mirabilis strains were investigated. P. mirabilis mutant R4/028 which completely lacked 4-amino-4-deoxy-L-arabinose was sensitive towards both polymyxins, and the other P. mirabilis strains investigated were resistant. Lipopolysaccharide (LPS) from Pmx-sensitive R4/028 strain, binds 50% more Pmx B than LPS derived from resistant P. mirabilis strains. The presence of iodoacetamide, N-ethylmaleimide and chloramphenicol rendered the Pmx-resistant P. mirabilis strains sensitive towards both polymyxins.
Kaca
W
W
Institute of Microbiology, University of Lódź, Poland.
Radziejewska-Lebrecht
J
J
Bhat
U R
UR
D002701:Chloramphenicol / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D005033:Ethylmaleimide / Q000494:pharmacology; D007460:Iodoacetamide / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000378:metabolism*; D009154:Mutation; D011112:Polymyxin B / Q000378:metabolism / Q000494:pharmacology*; D011113:Polymyxins / Q000378:metabolism / Q000494:pharmacology*; D011513:Proteus mirabilis / Q000187:drug effects* / Q000235:genetics
D008070:Lipopolysaccharides; D011113:Polymyxins; D002701:Chloramphenicol; D011112:Polymyxin B; D005033:Ethylmaleimide; D007460:Iodoacetamide