Monoclonal antibodies to the amino-terminal sequence of the c-yes gene product as specific probes of its expression.
An amino terminal sequence of 64 amino acid residues specific to human c-yes was expressed in E. coli as a fused molecule with a bacterial phosphate binding protein. The fused protein was used as both immunogen for mice and antigen to obtain monoclonal antibodies against the c-yes protein. Monoclonal antibodies (MAbs) from two monoclonal hybridomas (1B7: IgG2a, 3H9: IgG1) recognized human p62c-yes in blotting analyses. In addition, MAb3H9 reacted with murine p62c-yes. The p62c-yes immunoprecipitated with Mab1B7 exhibited protein tyrosine kinase and autophosphorylation activities in vitro. Immunohistochemical analysis with MAb1B7 showed that the p62c-yes expression in the kidney was confined to the proximal tubules.
Sukegawa
J
J
Department of Oncology, University of Tokyo, Japan.
Akatsuka
T
T
Sugawara
I
I
Mori
S
S
Yamamoto
T
T
Toyoshima
K
K
D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005333:Fetus; D015870:Gene Expression; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D007668:Kidney / Q000201:enzymology; D051379:Mice; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000276:immunology; D051575:Proto-Oncogene Proteins c-yes; D011519:Proto-Oncogenes; D014162:Transfection; D019061:src-Family Kinases
D000911:Antibodies, Monoclonal; D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; D051575:Proto-Oncogene Proteins c-yes; C496639:Yes1 protein, mouse; D019061:src-Family Kinases