title: Accuracy and reproducibility of lumbar bone mineral status determined by dual photon absorptiometry in live male cynomolgus macaques (Macaca fascicularis).
abstract: Dual photon absorptiometry (DPA) was used to determine the in vivo bone mineral content (BMC) and bone mineral density of lumbar vertebrae (L2-4) in feral adult male cynomolgus macaques (Macaca fascicularis, n = 20). Following in vivo DPA scans, all animals were euthanized and the lumbar spine segment excised. The excised lumbar vertebrae, plus spinal cord, were formalin-fixed and measured three times by ex vivo DPA. The first ex vivo scan matched the monkey's own in vivo soft tissue beam attenuation ratio (Rs, ranging from 1.38 to 1.45). The second ex vivo scan was made creating a constant obese-like condition (Rs = 1.38), and the third creating a constant lean-like condition (Rs = 1.45). All scans were taken at a 1.0 mm point resolution, 1.0 mm line spacing, a 6 cm width, and a scan speed of 5 mm/sec. The second lumbar vertebra (L2) was ashed and the ash weight was compared to the measured L2BMC of the in vivo and ex vivo DPA analyses. Noninvasive in vivo DPA proved to be adequate in measuring the lumbar bone mineral content in male cynomolgus macaques. Ex vivo DPA at a high and constant Rs value of 1.45 provided for the best accuracy of formalin-fixed wet bone tissue when compared to the bone ash weight.
authors.affiliation: Department of Comparative Medicine, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27103.
authors.forename: M J
authors.initials: MJ
authors.lastname: Jayo
authors.forename: D S
authors.initials: DS
authors.lastname: Weaver
authors.forename: S E
authors.initials: SE
authors.lastname: Rankin
authors.forename: J R
authors.initials: JR
authors.lastname: Kaplan
mesh_terms: D015502:Absorptiometry, Photon / Q000662:veterinary*; D000818:Animals; D015519:Bone Density; D008159:Lumbar Vertebrae / Q000032:analysis*; D008251:Macaca; D008252:Macaca fascicularis; D008297:Male; D015203:Reproducibility of Results