title: Inhibition of phorbol ester-induced neutrophil chemiluminescence by FeLV.
abstract: Receptor-mediated activation is accompanied by phospholipid metabolism and by calcium fluctuation resulting in a chemiluminescence (CL) response in the neutrophil. This pathway involves activation of protein kinase C (PKC) and the NADPH oxidase. Artificial stimulants such as phorbol esters, specifically 12-O-tetradecanylphorbol-13-acetate (TPA), circumvent the receptor-mediated pathway and activate PKC resulting in a measurable CL response. Neutrophils from feline leukemia virus (FeLV) exposed cats were tested for their ability to generate a TPA-induced CL response. As compared to the non-FeLV-exposed specific-pathogen-free (SPF) control cat neutrophil CL responses, both viremic and nonviremic FeLV-exposed cats showed significant decreases in their CL responsiveness. Neither ultraviolet light-inactivated FeLV (UV-FeLV) nor protein components (FeLV-p15E and FeLV-p27) caused a significant decrease in the CL responses of the SPF cat neutrophils. The suppressed TPA-induced CL response from FeLV-infected cats may involve an intracellular mechanism not affected in vitro by exposure of the neutrophil to the virus or viral components.
authors.affiliation: Department of Veterinary Pathobiology, Ohio State University, Columbus.
authors.forename: C S
authors.initials: CS
authors.lastname: Dezzutti
authors.forename: L J
authors.initials: LJ
authors.lastname: Lafrado
authors.forename: M G
authors.initials: MG
authors.lastname: Lewis
authors.forename: R G
authors.initials: RG
authors.lastname: Olsen
mesh_terms: D000818:Animals; D002415:Cats; D002469:Cell Separation; D005455:Fluorescent Antibody Technique; D005256:Leukemia Virus, Feline / Q000276:immunology* / Q000528:radiation effects; D007942:Leukemia, Experimental / Q000276:immunology / Q000382:microbiology; D008163:Luminescent Measurements; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D015682:Retroviridae Proteins, Oncogenic / Q000276:immunology / Q000302:isolation & purification; D013047:Specific Pathogen-Free Organisms; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014466:Ultraviolet Rays; D014766:Viremia / Q000276:immunology / Q000382:microbiology
chemical_list: D015682:Retroviridae Proteins, Oncogenic; C051525:feline leukemia virus protein p15E; D013755:Tetradecanoylphorbol Acetate