Unique tissue distribution of two distinct cellular retinoic acid binding proteins in neonatal and adult rat.
Two different species of cellular retinoic acid binding proteins, CRABP-I and CRABP-II, have been found in neonatal rat pups (Bailey, J. S. and Siu, C.-H. (1988) J. Biol. Chem. 263, 9326-9332). In this report, we describe a sensitive radio-ligand binding assay for CRABP in crude tissue extracts. The assay makes use of anion-exchange high-pressure liquid chromatography which effectively separates CRABP-II from CRABP-I, thus permitting the simultaneous quantitation of these two proteins. The distribution CRABP-I and CRABP-II in various neonatal and adult tissues of the rat has been examined. CRABP-I is the predominant species of CRABP and is present in high levels in the brain, skin and testis. CRABP-II is apparently unique to the skin of neonatal animals and it becomes undetectable in adult skin. Interestingly, CRABP-II is detected at a significant level only in the adrenals of adult animals, while neonatal adrenals express only CRABP-I and not CRABP-II. CRABP-I is present in higher levels in most organs at the neonatal stage than in the adult.
Bailey
J S
JS
Banting and Best Department of Medical Research, Charles H. Best Institute, University of Toronto, Canada.
Siu
C H
CH
D000311:Adrenal Glands / Q000378:metabolism; D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000378:metabolism; D002352:Carrier Proteins / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003600:Cytosol / Q000378:metabolism; D008297:Male; D011869:Radioligand Assay; D051381:Rats; D011920:Rats, Inbred WF; D018168:Receptors, Retinoic Acid; D012867:Skin / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D014018:Tissue Distribution
D002352:Carrier Proteins; D018168:Receptors, Retinoic Acid