Reduction of HSV-1 binding to BHK cells after treatment with phosphatidylinositol-specific phospholipase C.
Baby-hamster kidney cells were treated with unspecific and phosphatidylinositol-specific phospholipase C (PI-PLC) prior to infection with herpes simplex virus type 1 or 2. Subsequent to PI-PLC treatment, a 30% reduction of infectivity and receptor binding was observed for type 1 virus, while type 2 was unaffected. Treating the cells with unspecific phospholipase C did not affect subsequent infection with either virus. Treatment of the virus particles with the unspecific phospholipase reduced its infectivity, probably due to loss of the viral envelope. PI-PLC treatment of virus particles did not have any such effect on virus infectivity.
Langeland
N
N
Department of Biochemistry, Haukeland Hospital, University of Bergen, Norway.
Moore
L J
LJ
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism; D011991:Receptors, Virus / Q000378:metabolism*; D018139:Simplexvirus / Q000378:metabolism*; D010738:Type C Phospholipases / Q000494:pharmacology*
D008565:Membrane Proteins; D010716:Phosphatidylinositols; D011991:Receptors, Virus; D010738:Type C Phospholipases