Interleukin 3 as a trophic factor for central cholinergic neurons in vitro and in vivo.
We have found that interleukin 3 (IL-3), a growth factor for hematopoietic cells, is a novel trophic factor for mouse and rat central cholinergic neurons. It enhanced neurite outgrowth and elevated choline acetyltransferase activity. The effect seems to be specific for cholinergic neurons, since somatostatin release and glutamic acid decarboxylase and 2',3'-cyclic nucleotide 3'-phosphodiesterase activities were not significantly influenced by IL-3. In vivo, IL-3 was infused into the lateral ventricles of rats after unilateral axotomy of the septohippocampal pathways. Two weeks later, the IL-3-treated animals showed significant numbers of acetylcholinesterase-positive neurons remaining in the septal region.
Kamegai
M
M
Division of Demyelinating Disease and Aging, National Institute of Neuroscience, NCNP, Tokyo, Japan.
Niijima
K
K
Kunishita
T
T
Nishizawa
M
M
Ogawa
M
M
Araki
M
M
Ueki
A
A
Konishi
Y
Y
Tabira
T
T
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism; D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001369:Axons / Q000648:ultrastructure; D001685:Biological Factors / Q000494:pharmacology; D001921:Brain / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D002460:Cell Line; D002478:Cells, Cultured; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D016207:Cytokines; D004261:DNA Replication / Q000187:drug effects; D005968:Glutamate Decarboxylase / Q000378:metabolism; D007377:Interleukin-3 / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009414:Nerve Growth Factors / Q000494:pharmacology; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013936:Thymidine / Q000378:metabolism
D001685:Biological Factors; D016207:Cytokines; D007377:Interleukin-3; D009414:Nerve Growth Factors; D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D005968:Glutamate Decarboxylase; D013936:Thymidine