DNA-hybridization electron microscopy tertiary structure of 16 S rRNA.
Seven regions of 16 S rRNA have been located on the surface of the 30 S ribosomal subunit by DNA-hybridization electron microscopy. This information has been incorporated into a model for the tertiary structure of 16 S rRNA, accounting for approximately 40% of the total 16 S rRNA. A structure labeled the platform ring is proposed for a region of rRNA within the central domain. This structure rings the edges of the platform and includes regions 655-751 and 769-810. Another region, the recognition complex, consists of nucleotides 500 to 545, and occupies a region on the exterior surface of the subunit near the elongation factor Tu binding site. Ribosomal proteins that have been mapped by immunoelectron microscopy are superimposed onto the model in order to examine possible regions of interaction. Good correlation between the model locations of ribosomal proteins, and regions of rRNA protected by ribosomal proteins provide independent support for this model.
Oakes
M I
MI
Molecular Biology Institute, University of California, Los Angeles 90024.
Kahan
L
L
Lake
J A
JA
D001483:Base Sequence; D015342:DNA Probes; D004926:Escherichia coli / Q000378:metabolism / Q000648:ultrastructure; D046911:Macromolecular Substances; D008854:Microscopy, Electron / Q000379:methods; D008958:Models, Molecular; D008961:Models, Structural; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D012335:RNA, Ribosomal / Q000648:ultrastructure*; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000648:ultrastructure*; D012270:Ribosomes / Q000648:ultrastructure
D015342:DNA Probes; D046911:Macromolecular Substances; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S