title: Regulation and energization of nitrate transport in a halophilic Pseudomonas stutzeri.
abstract: Nitrate transport and its regulation by oxygen was studied in denitrifying halophilic Pseudomonas stutzeri, strain Zobell, and a Tn-5 transposon nitrite reductase mutant of this organism. The rate of nitrate transport was found to be 130 nanomoles nitrate min-1 mg protein-1 and 150 nanomoles nitrate min-1 mg protein-1 in the wildtype and the nitrite reductase mutant respectively as compared to 26.4 nanomoles nitrate min-1 mg protein-1 in a non-halophilic Pseudomonas stutzeri. Asparagine was found to be the best energy source for nitrate uptake. The ratio of nitrate import to nitrite export was established by measuring extracellular nitrate and nitrite concentrations using HPLC/UV analysis. There was a 1.3:1 (NO3-/NO2-) exchange. High concentrations of nitrate during growth was found to have a negative effect on nitrite metabolism. Oxygen exerted an inhibitory effect on nitrate uptake which was reversible and more pronounced in cells grown on low concentrations of nitrate compared to cells grown at high concentrations of nitrate.
authors.affiliation: University of Dayton, Department of Biology, Ohio 45469.
authors.forename: F M
authors.initials: FM
authors.lastname: Dias
authors.forename: R M
authors.initials: RM
authors.lastname: Ventullo
authors.forename: J J
authors.initials: JJ
authors.lastname: Rowe
mesh_terms: D000332:Aerobiosis; D001692:Biological Transport / Q000187:drug effects; D004251:DNA Transposable Elements; D006358:Hot Temperature; D007700:Kinetics; D009154:Mutation; D009566:Nitrates / Q000378:metabolism*; D009572:Nitrite Reductases / Q000235:genetics; D009573:Nitrites / Q000378:metabolism; D010100:Oxygen / Q000494:pharmacology; D011549:Pseudomonas / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D004251:DNA Transposable Elements; D009566:Nitrates; D009573:Nitrites; D009572:Nitrite Reductases; D010100:Oxygen