Base-specific binding of adenosine antibodies to double-stranded DNA.
Antibodies raised against adenosine have been reported to react with single-stranded DNA but not with double-stranded DNA. Using a highly sensitive avidin-biotin microELISA we report that these antibodies also react with double-stranded DNA. The binding was specific as it was completely inhibited by the homologous hapten. The results indicate that the antibody populations binding to ssDNA and dsDNA are not cross-reactive. The antibodies were shown to react with the topoisomers of plasmid DNA as assessed by gel retardation assay. The antibodies showed differential binding to restriction fragments of DNA indicating that some of the A residues in dsDNA are accessible to the antibodies.
Vaishnav
Y N
YN
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore.
Antony
A
A
D000241:Adenosine / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000918:Antibody Specificity / Q000276:immunology; D003429:Cross Reactions; D004247:DNA / Q000276:immunology*; D004262:DNA Restriction Enzymes; D004277:DNA, Single-Stranded / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D010957:Plasmids; D011817:Rabbits
D004277:DNA, Single-Stranded; D004247:DNA; D004262:DNA Restriction Enzymes; D000241:Adenosine