Serological responses of cats to feline immunodeficiency virus.
The proteins of feline immunodeficiency virus (FIV) were identified by sodium dodecylsulphate poly-acrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. Purified [35S]methionine/cysteine-labelled virus contained proteins of Mr 120, 24, 17, and 10kD, of which the most prominent were p24 and p17, and minor components of 62, 54, 52, 41 and 32kD. Sera from FIV-infected cats precipitated two glycoproteins (gp) of Mr 120kD (gp120) and 41kD (gp41) from lysates of [14C]glucosamine-labelled infected cells. Purified virus contained very little or no detectable glycoproteins. The serological response to individual viral proteins was followed in experimentally infected cats by immunoblotting. Since purified virus was a poor source of gp120, a method using FIV-infected cell lysates was developed. Cats produced antibodies to gp120, p55, p24 and p17. (The p55 was presumed to be a precursor of p24 and p17.) Following infection, antibodies developed first to p24 and subsequently to p17, p55 and gp120. Sera from cats infected with three separate isolates of FIV, two from the UK and one from the USA, had cross-reacting antibodies to all of these viral proteins. The criteria for identification of seropositive cats were defined. The minimum requirement for a positive immunoblot was antibody to gp120 or to at least three core proteins (p55, p24 and p17). Comparison of two commercial enzyme-linked immunosorbent assay (ELISA) kits and immunoblotting indicated that false-positive results occurred as a result of non-specific reactions in the ELISA systems.
Hosie
M J
MJ
Department of Veterinary Pathology, University of Glasgow, UK.
Jarrett
O
O
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002371:Cat Diseases / Q000276:immunology*; D002415:Cats; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006678:HIV / Q000276:immunology; D015151:Immunoblotting; D008970:Molecular Weight; D012190:Retroviridae / Q000276:immunology*; D012192:Retroviridae Infections / Q000276:immunology / Q000662:veterinary*; D012191:Retroviridae Proteins / Q000276:immunology / Q000302:isolation & purification
D000914:Antibodies, Viral; D012191:Retroviridae Proteins