[A rapid method for serodiagnosis of Japanese encephalitis using persistently infected C6/J-121 cells].
The usefulness of persistently infected C6/36 (C6/J-121) cells with Japanese encephalitis virus (JEV) for a rapid serodiagnostic test was examined with the sera of men, swine and laboratory animals by indirect immunofluorescent antibody (IFA) technique. Detection of specific antibodies was completed within 3 to 5 hours. Nonspecific fluorescence frequently observed in other IFA tests was few. The sensitivity of antibody detection and the serological specificity of IFA (IgG) were similar to those of hemagglutination-inhibition (HI) test. For the detection of IgM antibodies in sera of JE-patients and naturally JEV infected swine, this method was found to be simpler and more sensitive and rapid than the conventional HI test which required 2-ME treatment of the sera.
Kimura
T
T
Virsu Laboratory, Osaka Prefectural Institute of Public Health.
Otake
T
T
Ueba
N
N
Minekawa
Y
Y
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002478:Cells, Cultured; D004664:Encephalitis Virus, Japanese / Q000276:immunology; D004672:Encephalitis, Japanese / Q000175:diagnosis* / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D011237:Predictive Value of Tests; D013552:Swine
D000914:Antibodies, Viral