title: Production of cytokines by peripheral blood monocytes/macrophages infected with human immunodeficiency virus type 1 (HIV-1).
abstract: The study of monocyte/macrophage functions after human immunodeficiency virus type 1 (HIV-1) infection may help in understanding the pathogenesis of AIDS. The production of four cytokines, tumor necrosis factor alpha (TNF alpha), interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), and granulocyte-macrophage colony-stimulating factor (GM-CSF), by peripheral blood monocytes/macrophages was evaluated after in vitro infection with HIV-1. HIV-1 infection of these monocytes/macrophages did not result in release of any of these cytokines. Similarly, treatment of uninfected cells with purified recombinant HIV-1 envelope protein did not result in cytokine production. After stimulation with endotoxin or endotoxin plus interferon-gamma, HIV-1-infected monocytes/macrophages produced amounts of TNF alpha, IL-6, GM-CSF, and IL-1 beta comparable to that of uninfected cells. HIV-1 infection does not appear to induce or alter cytokine production by mononuclear phagocytes, which retain the capacity to produce these cytokines after endotoxin stimulation.
authors.affiliation: Division of Hematology/Oncology, New England Deaconess Hospital, Harvard Medical School, Boston, MA 02215.
authors.forename: J M
authors.initials: JM
authors.lastname: Molina
authors.forename: R
authors.initials: R
authors.lastname: Schindler
authors.forename: R
authors.initials: R
authors.lastname: Ferriani
authors.forename: M
authors.initials: M
authors.lastname: Sakaguchi
authors.forename: E
authors.initials: E
authors.lastname: Vannier
authors.forename: C A
authors.initials: CA
authors.lastname: Dinarello
authors.forename: J E
authors.initials: JE
authors.lastname: Groopman
mesh_terms: D001685:Biological Factors / Q000096:biosynthesis*; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000096:biosynthesis; D016207:Cytokines; D003588:Cytopathogenic Effect, Viral; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000096:biosynthesis; D015497:HIV-1 / Q000276:immunology* / Q000502:physiology; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D008264:Macrophages / Q000276:immunology / Q000382:microbiology*; D009000:Monocytes / Q000276:immunology / Q000382:microbiology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D014779:Virus Replication / Q000502:physiology
chemical_list: D001685:Biological Factors; D003115:Colony-Stimulating Factors; D016207:Cytokines; D006133:Growth Substances; D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016178:Granulocyte-Macrophage Colony-Stimulating Factor