Immunohistochemical localization of 54,000 dalton sialoglycoprotein in the epididymal duct of the germ cell-free W/Wv mouse.
A monoclonal antibody T21 specifically recognizes the mouse epididymal sialoglycoprotein of 54,000 dalton (SGP54). The localization of SGP54 was studied in the epididymal duct of germ cell-free WBB6F1W/Wv mutant mice (W/Wv mice) by avidin biotin complex (ABC) immunohistochemistry using T21. None of the testis cells showed immunoreaction. No spermatozoa were present in the epididymal duct lumen. The duct luminal fluid was stained weakly in the proximal corpus epididymidis, and strongly in the cauda epididymidis. Degenerated cells appeared in the duct lumen. The degenerated cells located at the corpus epididymidis showed strong immunostaining in the cytoplasmic region, while the degenerated cells located at the cauda epididymidis showed weak immunostaining. Immunoreaction was also detected between and on microvilli along the epididymis, the intensity being very strong at the distal caput and proximal corpus epididymidis. Invaginations and coated vesicles at the luminal surface of the principal cells were frequently immunostained at the corpus epididymidis. Giant inclusions frequently occurred in the principal cells of the distal caput and corpus epididymidis, with these being very intensely immunostained. These inclusions are ultrastructurally confirmed to be giant multivesicular bodies reported by ABE et al. (1984) in the mouse with the efferent duct cutting. These results suggest that the majority of excess SGP54 are absorbed by the principal cells at the distal caput to corpus epididymidis and catalyzed in the giant multivesicular bodies.
Toshimori
K
K
Department of Anatomy, Miyazaki Medical College, Japan.
Araki
S
S
Oura
C
C
D000818:Animals; D000911:Antibodies, Monoclonal; D003034:Coated Pits, Cell-Membrane / Q000032:analysis; D003593:Cytoplasm / Q000648:ultrastructure; D004822:Epididymis / Q000032:analysis* / Q000648:ultrastructure; D005856:Germ-Free Life; D007150:Immunohistochemistry; D002479:Inclusion Bodies / Q000032:analysis; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D008871:Microvilli / Q000032:analysis; D008970:Molecular Weight; D012795:Sialoglycoproteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D012795:Sialoglycoproteins