Regulated expression of the small nuclear ribonucleoprotein particle protein SmN in embryonic stem cell differentiation.
The SmN protein is a component of small nuclear ribonucleoprotein particles and is closely related to the ubiquitous SmB and B' splicing proteins. It is expressed in a limited range of tissues and cell types, including several undifferentiated embryonal carcinoma cell lines and undifferentiated embryonic stem cells. The protein declines to undetectable levels when embryonal carcinoma or embryonic stem cells are induced to differentiate, producing primitive endoderm or parietal endoderm or yielding embryonal bodies. This decline is due to a corresponding decrease in the level of the SmN mRNA. The potential role of SmN in the regulation of alternative splicing in embryonic cell lines and early embryos is discussed.
Sharpe
N G
NG
Department of Biochemistry, University College and Middlesex School of Medicine, London, United Kingdom.
Williams
D G
DG
Latchman
D S
DS
D000199:Actins / Q000235:genetics; D000818:Animals; D001324:Autoantigens / Q000096:biosynthesis / Q000235:genetics*; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D000242:Cyclic AMP / Q000494:pharmacology; D015342:DNA Probes; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D007700:Kinetics; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D012342:RNA, Small Nuclear / Q000235:genetics*; D012261:Ribonucleoproteins / Q000096:biosynthesis / Q000235:genetics*; D017411:Ribonucleoproteins, Small Nuclear; D014212:Tretinoin / Q000494:pharmacology; D055517:snRNP Core Proteins
D000199:Actins; D001324:Autoantigens; D015342:DNA Probes; D012333:RNA, Messenger; D012342:RNA, Small Nuclear; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; C526416:Snrpb protein, mouse; D055517:snRNP Core Proteins; D014212:Tretinoin; D000242:Cyclic AMP