title: Import into mitochondria of precursors containing hydrophobic passenger proteins: pretreatment of precursors with urea inhibits import.
abstract: We have studied the import into isolated yeast mitochondria of three hydrophobic passenger proteins attached to the N-terminal cleavable presequence of mitochondrial ATPase subunit 9 from Neurospora crassa. One natural precursor (pN9) contained N. crassa subunit 9; two chimaeric precursors, N9L/Y8-1 and N9L/Y9-2, respectively contained yeast mitochondrial ATPase subunits 8 and 9. In the absence of urea, pN9 and N9L/Y8-1 are imported efficiently but N9L/Y9-2 is not imported. After pretreatment of precursors in 4 M urea, binding of pN9 to mitochondria is marginally affected while its import is substantially inhibited; the binding to mitochondria of chimaeric proteins, N9L/Y8-1 and N9L/Y9-2, is greatly enhanced but no import is observed. This behaviour of import precursors containing hydrophobic passenger proteins is contrasted with that of a hydrophilic chimaeric precursor pCOXIV-DHFR, whose binding and import are enhanced by pretreatment with a high concentration of urea (8 M). The import of N9L/Y8-1 is very sensitive to the presence of low concentrations of urea in the import reaction mixture, and is abolished above 0.5 M urea although precursor binding to mitochondria is increased. By contrast, neither the import nor binding of pCOXIV-DHFR is affected directly by urea up to 0.8 M. These deleterious effects of urea on import of the chimaeric precursors N9L/Y8-1 and N9L/Y9-2 are interpreted in terms of a non-productive binding of these precursors to mitochondria, brought about by exposure of their hydrophobic domains resulting from urea unfolding. The generalization that membrane translocation of mitochondrial import precursors is enhanced by their prior unfolding in urea thus does not apply in the case of these precursors containing hydrophobic passenger proteins.
authors.affiliation: Department of Biochemistry, Monash University, Clayton, Victoria, Australia.
authors.forename: R H
authors.initials: RH
authors.lastname: Law
authors.forename: P
authors.initials: P
authors.lastname: Nagley
mesh_terms: D002678:Chimera; D003576:Electron Transport Complex IV / Q000235:genetics; D004792:Enzyme Precursors / Q000235:genetics* / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D009491:Neurospora / Q000378:metabolism*; D009492:Neurospora crassa / Q000187:drug effects / Q000378:metabolism*; D011487:Protein Conformation; D011489:Protein Denaturation; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics; D014508:Urea / Q000494:pharmacology*
chemical_list: D004792:Enzyme Precursors; D014508:Urea; D013762:Tetrahydrofolate Dehydrogenase; D003576:Electron Transport Complex IV