Specific effect of interferon on the herpes simplex virus type 1 transactivation event.
Human recombinant gamma interferon and to a lesser extent alpha interferon were shown to inhibit herpes simplex virus type 1 replication in the human cell lines WISH and HEp-2. Dot blot analysis of viral DNA synthesis and viral RNA transcription indicated that the inhibition occurred at an early step in infection. A study of the early events after herpes simplex virus type 1 infection indicated that adsorption, penetration, uncoating, and transport of viral DNA were not affected by interferon. Northern (RNA) blot analysis revealed that both immediate-early and delayed-early gene transcription was inhibited by interferon. Transactivation of the immediate-early responsive element linked to a reporter gene (CAT or tk) was specifically inhibited by both classes of interferon. Our data would indicate that either the transactivating protein VP16 or the complex formed between VP16 and a host protein(s) is attenuated by interferon.
De Stasio
P R
PR
Department of Biology, Indiana University, Bloomington 47405.
Taylor
M W
MW
D000818:Animals; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D010957:Plasmids; D011994:Recombinant Proteins; D015183:Restriction Mapping; D018139:Simplexvirus / Q000187:drug effects / Q000235:genetics*; D015533:Transcriptional Activation / Q000187:drug effects*; D014162:Transfection; D014709:Vero Cells; D010948:Viral Plaque Assay
D007370:Interferon Type I; D015345:Oligonucleotide Probes; D011994:Recombinant Proteins; D007371:Interferon-gamma