An enzyme-linked immunosorbent assay for detection of bovine leukaemia virus p24 antibody in cattle.
An ELISA for detecting antibody to the bovine leukaemia virus (BLV) core protein p24 is described. The test uses p24 antigen purified from concentrated cell culture supernate by lectin-affinity chromatography and gel filtration. The sensitivity and specificity of the p24-ELISA for diagnosing BLV infection relative to the gp51 agar gel immunodiffusion test, were 98.1 and 96.7%, respectively. In the event of widespread use of gp51 based vaccines, the p24-ELISA should differentiate effectively between naturally infected and vaccinated animals.
Molloy
J B
JB
Queensland Department of Primary Industries, Animal Research Institute, Yeerongpilly, Australia.
Walker
P J
PJ
Baldock
F C
FC
Rodwell
B J
BJ
Cowley
J A
JA
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D007938:Leukemia / Q000175:diagnosis / Q000662:veterinary*; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D012190:Retroviridae / Q000276:immunology*; D015682:Retroviridae Proteins, Oncogenic / Q000276:immunology*; D014758:Viral Core Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D015682:Retroviridae Proteins, Oncogenic; D014758:Viral Core Proteins