Histochemical method for simultaneous fiber typing and demonstration of capillaries in skeletal muscle.
Since capillary density of skeletal muscle depends on fiber type distribution, fiber typing should accompany measurement of capillary density. To obviate the need for multiple sectioning, we suggest NADH tetrazolium reductase enzyme histochemistry for fiber typing followed (on the same slide) by lectin histochemistry to demonstrate the binding of Ulex europaeus agglutinin, a sensitive marker for endothelium. The method is quick, highly reproducible and gives density estimates comparable to earlier, more tedious methods.
Paljärvi
L
L
Department of Pathology, University Central Hospital of Kuopio, Finland.
Naukkarinen
A
A
D000328:Adult; D002196:Capillaries / Q000378:metabolism; D005260:Female; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D007124:Immunoenzyme Techniques; D037102:Lectins / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009132:Muscles / Q000098:blood supply*; D009246:NADH Tetrazolium Reductase / Q000378:metabolism
D037102:Lectins; D009246:NADH Tetrazolium Reductase