Mini-Mu mediates deletion-inversions in vivo by intra-transposon transposition.
We have shown that a mini-Mu can transpose into itself in vivo to generate a circle containing only transposon sequences. This deletion-inversion product, which has previously been observed in vitro, is formed by non-replicative transposition and has directly repeated Mu ends. It therefore cannot undergo further rounds of transposition and retains the two copies of the target sequence duplicated in the event. Thus we have been able to confirm that a mini-Mu can undergo non-replicative reactions in vivo and that these generate a 5 bp target site duplication, as has been shown to occur following replicative transposition and lysogenization with Mu.
Leach
D R
DR
Department of Microbiology, University of Edinburgh, UK.
Okely
E A
EA
Percy-Robb
M I
MI
D010583:Bacteriophage mu / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D002872:Chromosome Deletion; D007446:Chromosome Inversion; D004251:DNA Transposable Elements; D004270:DNA, Circular / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D008969:Molecular Sequence Data; D005810:Multigene Family; D010957:Plasmids; D014775:Virus Activation
D004251:DNA Transposable Elements; D004270:DNA, Circular; D004279:DNA, Viral