Sequence analysis of the HN gene of parainfluenza virus type 2.
A cDNA library was constructed in lambda gt10 using mRNA purified from cells infected with parainfluenza virus type 2 (PIV2). Virus-specific clones were identified by screening the library with 32P-labelled cDNA probes made from randomly primed vRNA. Clones containing the haemagglutinin-neuraminidase (HN) gene were identified by sequence comparisons with known parainfluenza virus HN gene sequences. The largest HN clone isolated had a nucleic acid sequence of 2065 bp with a single long open reading frame encoding a protein of 571 amino acids. The HN protein has nine predicted glycosylation sites and an amino-terminal membrane-spanning region. The PIV2 HN protein shares 43% amino acid identity with the HN protein of simian virus 5 and 40% with mumps virus, 30% of the amino acids being common to all three viruses.
Precious
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B
Department of Biochemistry and Microbiology, University of St Andrews, Fife, U.K.
Southern
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JA
Randall
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RE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D015723:Gene Library; D005814:Genes, Viral; D015707:HN Protein / Q000235:genetics*; D008969:Molecular Sequence Data; D009109:Mumps virus / Q000235:genetics; D010223:Parainfluenza Virus 2, Human / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D018108:Respirovirus / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014709:Vero Cells
D015707:HN Protein; D012333:RNA, Messenger; D012367:RNA, Viral; D004247:DNA