Regulation of inhibin alpha- and beta B-subunit mRNA levels in rat Sertoli cells.
The hormonal regulation of the expression of the inhibin alpha-subunit and beta B-subunit genes was studied in cultured rat Sertoli cells. The alpha-subunit mRNA level increased during incubation of the cells in the presence of follicle-stimulating hormone (FSH), reaching maximal levels within 1.5 h. This stimulation was mimicked by addition of dibutyryl-cyclic AMP, indicating that FSH action on the alpha-subunit gene is exerted via cyclic AMP. Inhibition of translation by cycloheximide (CX) caused upregulation of the alpha-subunit mRNA, and did not block the effect of FSH on the level of this mRNA. In FSH-stimulated cells, the half-life of the alpha-subunit mRNA was 6 h, and this half-life was prolonged by inhibition of transcription using actinomycin D (AD). It is concluded that the effect of FSH on alpha-subunit mRNA expression represents a direct effect on the alpha-subunit gene, and that alpha-subunit mRNA levels are influenced by a short-lived mRNA destabilizing protein. The levels of two beta B-subunit mRNAs (4.2 kb and 3.5 kb) were not affected by FSH or dbcAMP. However, these mRNAs were also upregulated by CX treatment. Experiments using AD showed that the 4.2 kb mRNA is less stable than the 3.5 kb mRNA. The differential regulation of the inhibin alpha- and beta B-subunit mRNAs is discussed.
Klaij
I A
IA
Department of Biochemistry II, Medical Faculty, Erasmus University Rotterdam, The Netherlands.
Toebosch
A M
AM
Themmen
A P
AP
Shimasaki
S
S
de Jong
F H
FH
Grootegoed
J A
JA
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000494:pharmacology*; D003513:Cycloheximide / Q000494:pharmacology; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D005786:Gene Expression Regulation; D007265:Inhibins / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012708:Sertoli Cells; D014158:Transcription, Genetic / Q000187:drug effects
D000900:Anti-Bacterial Agents; D012333:RNA, Messenger; D007265:Inhibins; D005640:Follicle Stimulating Hormone; D003513:Cycloheximide; D000242:Cyclic AMP