SV40-mediated tumor selection and chromosome transfer to enrich for cystic fibrosis region.
The somatic cell hybrid C121, with chromosome 7 as its sole human component, arose when mouse macrophages SV40 genomes are integrated at 7q31-7q35. We show that hybrids with a reduced chromosome 7 component, but which retain markers linked to the cystic fibrosis locus, can be generated by direct in vivo tumor selection or following chromosome-mediated gene transfer and SV40-mediated cellular transformation. Our methods for chromosome fragmentation and fine-structure mapping can now be applied to the substantial number of SV40-transformed human cell lines, with independent chromosomal integration sites, already available. Our results also suggest that expression of human epidermal growth factor receptor augments the tumorigenic potential of the SV40-transformed C121 hybrid.
Porteous
D J
DJ
MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK.
Dorin
J R
JR
Wilkinson
M M
MM
Fletcher
J M
JM
Emslie
E
E
van Heyningen
V
V
D000818:Animals; D000941:Antigens / Q000032:analysis; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D002472:Cell Transformation, Viral / Q000235:genetics*; D002874:Chromosome Mapping; D002897:Chromosomes, Human, Pair 7; D003550:Cystic Fibrosis / Q000235:genetics*; D015342:DNA Probes; D066246:ErbB Receptors / Q000096:biosynthesis; D005821:Genetic Techniques; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D011519:Proto-Oncogenes / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection
D000941:Antigens; D015342:DNA Probes; D066246:ErbB Receptors