title: Biochemical and immunological characterization of collagen molecules from echinothurioid sea urchin Asthenosoma ijimai.
abstract: Collagens collected from the test (the external hard covering of invertebrates) of the sea urchin, Asthenosoma ijimai, were characterized biochemically and immunologically. The amino-acid composition was typical of that of mammalian collagens. Crystals of segment-long-spacing showed that the molecules of sea urchin collagen were 300 nm long. Selective salt precipitation revealed that the collagen has the same solubility characteristics as type I collagen. The collagen was denatured at 23.1 degrees C. Anti-sea urchin collagen antisera were immunologically cross-reacted with collagens of the same species and the starfish Asterina pectinifera. However, the antisera showed no or slight responses to collagens of bovine type I, II, III, IV and V. The collagen molecules contained four alpha-chains, named alpha 1(SU), alpha 2(SU), alpha 3(SU) and alpha 4(SU), respectively. All of the four alpha-chains were eluted in the same fraction on gel filtration chromatography. Chains of alpha 1(SU) and alpha 2(SU) were extracted earlier than alpha 3(SU) and alpha 4(SU) during pepsin digestion. Other biochemical and immunological analyses clearly demonstrated that test of sea urchins contains two genetically different, but biochemically similar, species of collagens, one of which is composed of alpha 1(SU) and alpha 2(SU) chains, and the other of alpha 3(SU) and alpha 4(SU).
authors.affiliation: Department of Biology, Faculty of Science, Tokyo Metropolitan University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Shimizu
authors.forename: S
authors.initials: S
authors.lastname: Amemiya
authors.forename: K
authors.initials: K
authors.lastname: Yoshizato
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D011232:Chemical Precipitation; D002852:Chromatography, Ion Exchange; D002942:Circular Dichroism; D003094:Collagen / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D008970:Molecular Weight; D012617:Sea Urchins / Q000032:analysis*
chemical_list: D002241:Carbohydrates; D003094:Collagen