title: [Phosphorylation of proteins in the kidney papillary zone by endogenous cAMP-dependent protein kinases].
abstract: Using SDS-PAAG electrophoresis with subsequent autoradiography, several proteins from plasma membranes and cell cytosol of rat kidney papillary zone were identified as substrates for endogenous cAMP-dependent protein kinases. The cAMP-dependent phosphorylation of plasma membrane proteins was made possible only after the destruction of membrane vesicles. Plasma membrane and cytosol fractions were found to contain a 58 kDa protein whose properties are similar to those of the regulatory subunit of cAMP-dependent protein kinase of the second type. It was shown also that the content of endogenous substrates of cAMP-dependent protein kinases in cell cytosol is higher than that in plasma membranes.
authors.forename: S G
authors.initials: SG
authors.lastname: Dzgoev
authors.forename: L N
authors.initials: LN
authors.lastname: Ivanova
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D007679:Kidney Medulla / Q000201:enzymology / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D008970:Molecular Weight; D010766:Phosphorylation; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014667:Vasopressins / Q000502:physiology
chemical_list: D008565:Membrane Proteins; D047428:Protein Kinase Inhibitors; D011506:Proteins; D014667:Vasopressins; D000242:Cyclic AMP; D011494:Protein Kinases
vernacular_title: Fosforilirovanie belkov papilliarnoĭ zony pochki éndogennymi cAMP-zavisimymi proteinkinazami.