Mineralocorticoid receptor gene expression in the gastrointestinal tract: distribution and ontogeny.
The gastrointestinal tract is a well characterized target tissue for aldosterone, where it regulates electrolyte transport, particularly in the descending colon. Previous studies have demonstrated the presence of aldosterone receptors in gastrointestinal tissues. We have used specific cRNA probes for the rat mineralocorticoid receptor to explore both the distribution and ontogeny of mineralocorticoid receptor gene expression in the gastrointestinal tract. Mineralocorticoid receptor gene expression is found throughout the small and large intestine, but is absent from the stomach. The highest levels are observed in the distal colon, and significant expression is found in the duodenum; in both tissues levels of expression are higher than those in kidney. In both the developing duodenum and colon, mineralocorticoid receptor gene expression precedes the development of the full physiological response to aldosterone. These findings emphasise the colon as an important target tissue for aldosterone, and raise the question of potential roles for aldosterone in the duodenum.
Fuller
P J
PJ
Medical Research Centre, Prince Henry's Hospital, Melbourne, Australia.
Verity
K
K
D000818:Animals; D003106:Colon / Q000254:growth & development / Q000378:metabolism; D004064:Digestive System / Q000254:growth & development / Q000378:metabolism*; D004386:Duodenum / Q000254:growth & development / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D015870:Gene Expression; D007668:Kidney / Q000378:metabolism; D008297:Male; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D018161:Receptors, Mineralocorticoid; D011987:Receptors, Steroid / Q000235:genetics*; D014018:Tissue Distribution
D015347:RNA Probes; D012333:RNA, Messenger; D018161:Receptors, Mineralocorticoid; D011987:Receptors, Steroid