Excision of the En/Spm transposable element of Zea mays requires two element-encoded proteins.
An excision assay system for En/Spm was developed in transgenic tobacco. The characteristics of excision and integration are similar to the natural system of Zea mays. In this transgenic model system two En/Spm encoded trans-acting functions, TNPA and TNPD, are required for excision. A biochemical model for transposition is proposed that might also be applicable to other transposable elements.
Frey
M
M
Max-Planck-Institut für Züchtungsforschung, Molekulare Pflanzengenetik, Köln, FRG.
Reinecke
J
J
Grant
S
S
Saedler
H
H
Gierl
A
A
D003001:Cloning, Molecular; D003433:Crosses, Genetic; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics*; D010947:Plants, Toxic; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins; D015183:Restriction Mapping; D014026:Nicotiana / Q000235:genetics; D014158:Transcription, Genetic; D003313:Zea mays / Q000235:genetics*
D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D015345:Oligonucleotide Probes; D010940:Plant Proteins; D012097:Repressor Proteins