Are there isoenzymes of cytochrome c oxidase in Paracoccus denitrificans?
We have used a gene replacement strategy to delete the previously isolated gene [(1987) EMBO J. 6, 2825-2833] for the cytochrome c oxidase subunit I from Paracoccus denitrificans. The resulting mutant was still able to synthesize active cytochrome c oxidase. This led us to look for another locus which could completely suppress the mutation. In this study we report the isolation of a second gene encoding subunit I. An open reading frame coding for cytochrome c 550 was found upstream from this gene. We suggest that there are isoenzymes of cytochrome c oxidase (cytochrome aa3) in this bacterium.
Raitio
M
M
Department of Medical Chemistry, University of Helsinki, Finland.
Pispa
J M
JM
Metso
T
T
Saraste
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010231:Paracoccus denitrificans / Q000201:enzymology* / Q000235:genetics; D010957:Plasmids; D015183:Restriction Mapping
D004269:DNA, Bacterial; D007527:Isoenzymes; D003576:Electron Transport Complex IV