Functional modes of retinoic acid in mouse osteoblastic clone MC3T3-E1, proved as a target cell for retinoic acid.
Mouse osteoblastic clone MC3T3-E1 was proved as a target cell for retinoic acid (RA) in bone tissues through the demonstration of RA-receptor gene expression by the northern blot analysis. The effect of RA on the cell growth of MC3T3-E1 was repressive for both subconfluent and confluent growth, whereas RA enhancement of alkaline phosphatase expression was observed at the confluent stage. This implies that RA is a regulatory factor leading osteogenesis of the cells after the confluent stage. RA exhibited simultaneously the stage-dependent effects on EGF-dependent mitogenesis: promotive at the subconfluent, but repressive at the confluent stage.
Nakayama
Y
Y
Okayama University Dental School, Okayama City, Japan.
Takahashi
K
K
Noji
S
S
Muto
K
K
Nishijima
K
K
Taniguchi
S
S
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004815:Epidermal Growth Factor / Q000378:metabolism / Q000494:pharmacology; D015870:Gene Expression; D051379:Mice; D009693:Nucleic Acid Hybridization; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D018168:Receptors, Retinoic Acid; D014212:Tretinoin / Q000494:pharmacology*
D002352:Carrier Proteins; D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; D014212:Tretinoin; D004815:Epidermal Growth Factor; D000469:Alkaline Phosphatase