Transcriptional induction of purple membrane and gas vesicle synthesis in the archaebacterium Halobacterium halobium is blocked by a DNA gyrase inhibitor.
We have investigated the expression of the bacteriorhodopsin gene (bop) and the gas vesicle protein gene (gvpA) in the extremely halophilic archaebacterium Halobacterium halobium, using primer-directed reverse transcription of RNA to quantify message levels. The level of gvpA gene transcript was found to increase about 5-fold from early to mid-logarithmic growth phase, while the level of bop gene transcript increased about 20-fold from mid-logarithmic to stationary phase. Transcriptional induction of both the gvpA and bop genes was significantly reduced by aeration and almost completely blocked by the DNA gyrase inhibitor novobiocin.
Yang
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CF
Department of Microbiology, University of Massachusetts, Amherst 01003.
DasSarma
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D019843:Archaeal Proteins; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D001436:Bacteriorhodopsins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D015870:Gene Expression / Q000187:drug effects; D006217:Halobacterium / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D007700:Kinetics; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009675:Novobiocin / Q000494:pharmacology; D015345:Oligonucleotide Probes; D011506:Proteins; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D059005:Topoisomerase II Inhibitors; D014158:Transcription, Genetic
D019843:Archaeal Proteins; D001425:Bacterial Outer Membrane Proteins; D008565:Membrane Proteins; D015345:Oligonucleotide Probes; D011506:Proteins; D012333:RNA, Messenger; D059005:Topoisomerase II Inhibitors; C038524:gas vesicle protein; D009675:Novobiocin; D001436:Bacteriorhodopsins