Factors involved in specific transcription by mammalian RNA polymerase II. Purification and subunit composition of transcription factor IIF.
Transcription factor IIF (TFIIF), a protein factor required for transcription of class II genes, has been purified to near homogeneity. TFIIF is a heterodimer of 30- and 78-kDa polypeptides. Both the 30- and 78-kDa polypeptides were necessary to reconstitute TFIIF activity. TFIIF activity eluted from a gel filtration column with an apparent molecular mass of 220 kDa, suggesting that the native factor exists as a dimer of the heterodimer.
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Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854-5635.
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Reinberg
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D000818:Animals; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D007163:Immunosorbent Techniques; D046911:Macromolecular Substances; D008970:Molecular Weight; D010766:Phosphorylation; D012319:RNA Polymerase II / Q000378:metabolism*; D014157:Transcription Factors / Q000302:isolation & purification* / Q000378:metabolism; D019473:Transcription Factors, TFII; D014158:Transcription, Genetic
D046911:Macromolecular Substances; D014157:Transcription Factors; D019473:Transcription Factors, TFII; D012319:RNA Polymerase II; C056604:transcription factor TFIIF