Human fibroblasts show expression of the leukotriene-A4-hydrolase gene, which is increased after simian-virus-40 transformation.
Human fibroblasts in cell culture converted the epoxide intermediate leukotriene A4 into the potent chemotaxin leukotriene B4. The identity of leukotriene B4 was ascertained by its mobility in reverse-phase high performance liquid chromatography, ultraviolet spectroscopy and gas chromatography/mass spectrometry. The presence of the enzyme responsible for the conversion (i.e. leukotriene A4 hydrolase), as well as the corresponding mRNA, were demonstrated by Western and Northern blot analyses. Leukotriene-A4-hydrolase enzyme activity, protein and mRNA were all enhanced (approximately threefold) in human fibroblasts that had been transformed by simian virus 40.
Medina
J F
JF
Department of Physiological Chemistry, karolinska Institutet, Stockholm, Sweden.
Barrios
C
C
Funk
C D
CD
Larsson
O
O
Haeggström
J
J
Rådmark
O
O
D002460:Cell Line; D002472:Cell Transformation, Viral / Q000235:genetics*; D004247:DNA / Q000032:analysis; D004851:Epoxide Hydrolases / Q000032:analysis / Q000235:genetics*; D005347:Fibroblasts / Q000201:enzymology; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D015151:Immunoblotting; D017572:Leukotriene A4; D007975:Leukotriene B4 / Q000032:analysis / Q000096:biosynthesis; D015289:Leukotrienes / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis*; D013539:Simian virus 40; D014764:Viral Proteins / Q000032:analysis
D017572:Leukotriene A4; D015289:Leukotrienes; D012333:RNA, Messenger; D014764:Viral Proteins; D007975:Leukotriene B4; D004247:DNA; D004851:Epoxide Hydrolases; C043134:leukotriene A4 hydrolase