title: Leukotriene B4 generation by human monocytes and neutrophils stimulated by uropathogenic strains of Escherichia coli.
abstract: The generation of the 5-lipoxygenase product, leukotriene B4 (LTB4) by human mononuclear phagocytes (monocytes) following incubation with 25 different uropathogenic strains of Escherichia coli correlated with the haemolytic activity of the strains (r = 0.572, P less than 0.01). LTB4 generation by human neutrophils (PMN), however, was unrelated to this haemolytic potential (r = 0.164). In contrast, both prelabelled monocytes and PMN were stimulated by haemolytic strains of E. coli and by haemolytic culture supernatants to release significant amounts of [3H]arachidonic acid. There was a significant correlation between haemolytic activity and [3H]arachidonic acid release generated by individual strains from monocytes (r = 0.804, P less than 0.001) and PMN (r = 0.888, P less than 0.001). In addition, nonhaemolytic strains but not their culture supernatants were capable of causing slow release of both [3H]arachidonic acid and LTB4 from PMN and mononuclear cells. These results suggest that both the possession of haemolytic activity, and the direct interaction of bacteria with the leukocyte surface are mechanisms by which uropathogenic strains of E. coli may cause the release and metabolism of arachidonic acid. In addition, there was synergistic augmentation by nonhaemolytic bacteria of the PMN LTB4 response to haemolytic culture supernatants or to low doses of the calcium ionophore A23187. These results support an ionophore-like mechanism for the activation of the cell by haemolysin. LTB4 generation by PMN incubated with haemolytic supernatants was also augmented by particulate zymosan in a manner dependent on the dose of zymosan, suggesting that the direct interaction of E. coli with PMN may involve an activation mechanism similar to that for zymosan. These results demonstrate differing responses of peripheral mononuclear cells and PMN from the same donors to identical strains of E. coli and suggest that the generation of the potent chemotactic agent LTB4 in response to E. coli infection in vivo need not depend solely on the elaboration of cytotoxic haemolysins by individual strains.
authors.affiliation: Institute of Nephrology, Kidney Research Unit Foundation, University of Wales College of Medicine, Cardiff Royal Infirmary, U.K.
authors.forename: R
authors.initials: R
authors.lastname: Steadman
authors.forename: N
authors.initials: N
authors.lastname: Topley
authors.forename: J
authors.initials: J
authors.lastname: Knowlden
authors.forename: B
authors.initials: B
authors.lastname: Spur
authors.forename: J
authors.initials: J
authors.lastname: Williams
mesh_terms: D000255:Adenosine Triphosphate / Q000302:isolation & purification; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D004926:Escherichia coli / Q000502:physiology*; D006461:Hemolysis; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007975:Leukotriene B4 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D009000:Monocytes / Q000378:metabolism* / Q000382:microbiology; D009504:Neutrophils / Q000378:metabolism* / Q000382:microbiology
chemical_list: D001095:Arachidonic Acids; D007975:Leukotriene B4; D016718:Arachidonic Acid; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase