Osmotic state of lenses in three dominant murine cataract mutants.
Three newly detected dominant cataract mutations (Asc-1, Cat-3vao, Tcm) were investigated for effects on osmotic alterations in the lenses of heterozygotes. The lens wet weight was reduced in two mutant lines (Cat-3vao and Tcm), and the water content in the lenses of the Cat-3vao mice was increased. Moreover, in the cataractous lenses from Cat-3vao mice, the sodium-potassium-adenosine triphosphatase (Na(+)-K(+)-ATPase) activity was enhanced and the ATP concentration, correspondingly decreased. The osmotic variations observed in the Cat-3vao mutants might have been due to a metabolic response to the yet unknown, primary pathological event. The lenses of the other two mutant lines (Asc-1 and Tcm) revealed no alterations that could be related to osmotic stress. In no mutant line investigated could a decrease in Na(+)-K(+)-ATPase activity be demonstrated that was similar to the causative factor in the Nakano mutant line. The Cat-3vao mice exhibited some similarities to the Philly mutant line.
Graw
J
J
GSF-Institut für Säugetiergenetik, Neuherberg, Federal Republic of Germany.
Reitmeir
P
P
Wulff
A
A
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001834:Body Water / Q000378:metabolism; D002386:Cataract / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005799:Genes, Dominant; D007908:Lens, Crystalline / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D009929:Organ Size; D009994:Osmolar Concentration; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D000255:Adenosine Triphosphate; D000254:Sodium-Potassium-Exchanging ATPase