title: Human meningiomas co-express platelet-derived growth factor (PDGF) and PDGF-receptor genes and their protein products.
abstract: The present studies investigated the expression of the platelet-derived growth factor (PDGF) and PDGF-receptor genes in human meningiomas. Northern blot analysis demonstrated that all meningiomas examined expressed both the c-sis/PDGF-2 proto-oncogene and the PDGF-receptor gene. In situ hybridization localized the c-sis mRNA and the PDGF-receptor mRNA in the tumor cells of the meningioma tissues. Control pachymeninges derived from adult individuals, without meningiomas, expressed only PDGF-receptor mRNA but not the c-sis mRNA. Immunocytochemistry studies detected both the c-sis and the PDGF-receptor protein products in meningioma tissues but only the PDGF-receptor protein products in control pachymeninges. These findings indicate the presence of an autocrine mechanism in human meningiomas based on the co-expression of the c-sis/PDGF-2 proto-oncogene and PDGF-receptor gene and their protein products. This co-expression of a potent mitogen and its receptor may contribute to the growth and maintenance of human meningiomas.
authors.affiliation: Center for Blood Research, Brigham and Women's Hospital, Boston, MA.
authors.forename: M
authors.initials: M
authors.lastname: Maxwell
authors.forename: T
authors.initials: T
authors.lastname: Galanopoulos
authors.forename: E T
authors.initials: ET
authors.lastname: Hedley-Whyte
authors.forename: P M
authors.initials: PM
authors.lastname: Black
authors.forename: H N
authors.initials: HN
authors.lastname: Antoniades
mesh_terms: D015152:Blotting, Northern; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics* / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D008577:Meningeal Neoplasms / Q000235:genetics* / Q000378:metabolism; D008579:Meningioma / Q000235:genetics* / Q000378:metabolism; D009364:Neoplasm Recurrence, Local / Q000235:genetics* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D015513:Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000235:genetics* / Q000378:metabolism; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D017479:Receptors, Platelet-Derived Growth Factor
chemical_list: C000715470:MAS1 protein, human; D015513:Oncogene Proteins; D010982:Platelet-Derived Growth Factor; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011956:Receptors, Cell Surface; D017479:Receptors, Platelet-Derived Growth Factor