[The role of the complex formation between cytochrome c and NAD in the manifestation of the photoprotective effect of the dinucleotide in relation to hemeprotein].
UV irradiation of free ferricytochrome solutions, pH 8, induces photorecovery of protein molecules. Hemoproteide photorecovery does not occur after irradiation of the ferricytochrome c/NAD mixture, pH 6 and 8: dinucleotide exerts a photoprotective effect with respect to ferricytochrome. This NAD effect is not observed after exposure of the ferricytochrome c/NAD system, pH 4. With this pH value, each component of the above mixture is eluted from a gel chromatographic column by its peak, whereas with pH 6 and 8, NAD and ferricytochrome c leave the column as one fraction. This indicates that the photoprotective effect of the coenzyme manifests itself upon formation of complex with hemoprotein.
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D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D003574:Cytochrome c Group / Q000032:analysis / Q000494:pharmacology / Q000528:radiation effects*; D004579:Electron Transport / Q000187:drug effects / Q000528:radiation effects; D006420:Hemeproteins / Q000187:drug effects* / Q000528:radiation effects; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D009243:NAD / Q000032:analysis / Q000494:pharmacology / Q000528:radiation effects*; D010084:Oxidation-Reduction / Q000187:drug effects / Q000528:radiation effects; D011837:Radiation-Protective Agents; D012996:Solutions; D013053:Spectrophotometry; D013696:Temperature; D014466:Ultraviolet Rays
D003574:Cytochrome c Group; D006420:Hemeproteins; D011837:Radiation-Protective Agents; D012996:Solutions; D009243:NAD
O roli kompleksoobrazovaniia mezhdu tsitokhromom c i NAD v proiavlenii fotozashchitnogo éffekta dinukleotida po otnosheniiu k gembelku.