title: A recombinant fowlpox virus expressing the hemagglutinin-neuraminidase gene of Newcastle disease virus (NDV) protects chickens against challenge by NDV.
abstract: The hemagglutinin-neuraminidase (HN) gene from the Beaudette C strain of Newcastle disease virus (NDV) has been expressed in a recombinant fowlpox virus vector. The HN gene, under the control of the vaccinia p7.5 promoter, was inserted into a nonessential gene in the terminal inverted repeats of fowlpox virus. Expression was demonstrated in tissue culture, a protein of the correct size for fully glycosylated HN protein being recognized by an HN-specific monoclonal antibody on Western blots. When the recombinant fowlpox virus was inoculated into chickens by intravenous or wing-web routes, antibody which recognizes HN from purified NDV virions was produced. Protective immunity to NDV was generated in the chickens; at the highest dose of vaccine 100% of the chickens tested were protected against challenge with a virulent strain of NDV.
authors.affiliation: Institute for Animal Health, Houghton Laboratory, Huntingdon, Cambridgeshire, United Kingdom.
authors.forename: M E
authors.initials: ME
authors.lastname: Boursnell
authors.forename: P F
authors.initials: PF
authors.lastname: Green
authors.forename: A C
authors.initials: AC
authors.lastname: Samson
authors.forename: J I
authors.initials: JI
authors.lastname: Campbell
authors.forename: A
authors.initials: A
authors.lastname: Deuter
authors.forename: R W
authors.initials: RW
authors.lastname: Peters
authors.forename: N S
authors.initials: NS
authors.lastname: Millar
authors.forename: P T
authors.initials: PT
authors.lastname: Emmerson
authors.forename: M M
authors.initials: MM
authors.lastname: Binns
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002645:Chickens / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D003001:Cloning, Molecular; D046508:Culture Techniques; D005587:Fowlpox virus / Q000235:genetics*; D005814:Genes, Viral; D005822:Genetic Vectors; D015707:HN Protein / Q000096:biosynthesis / Q000235:genetics*; D009522:Newcastle disease virus / Q000201:enzymology / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D014765:Viral Vaccines
chemical_list: D000911:Antibodies, Monoclonal; D015707:HN Protein; D014765:Viral Vaccines