[Effect of Ca2+- and K+-channel blockers on ACTH-stimulated steroidogenesis of isolated adrenal glands of adult and old rats].
Ca2+ channel blocker verapamil (0.2 mM) and K+ channel blocker 2-aminopyridine (1.0 mM; 5.0 mM and 10.0 mM) have been studied for their effect on ACTH-stimulated steroidogenesis in isolated adrenal glands (IAG) of the adult (6-7 months) and old (26-28 months) male rats. The administration of verapamil produced a similar, about 2-fold decrease in ACTH-stimulated secretion of 11-OCS in the IAG of both animal groups. 2-aminopyridine, administered in several concentrations, produced no effect whatsoever on ACTH-stimulated secretion of 11-OCS in the IAG of the old rats. However, when administered in concentrations of 5.0 mM and 10.0 mM this agent decreased significantly the ACTH-stimulated steroidogenesis in the IAG of the adult animals. It has been suggested that plasma membrane permeability of z. fasciculata adrenocorticocytes to K+ ions decreases with age.
Gorban'
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EN
Institute of Gerontology, Academy of Medical Sciences of the USSR, Kiev.
D015062:11-Hydroxycorticosteroids / Q000096:biosynthesis* / Q000378:metabolism; D000311:Adrenal Glands / Q000187:drug effects* / Q000378:metabolism; D000324:Adrenocorticotropic Hormone / Q000502:physiology*; D000375:Aging / Q000502:physiology*; D000631:Aminopyridines / Q000494:pharmacology*; D000818:Animals; D066298:In Vitro Techniques; D008297:Male; D015221:Potassium Channels / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D014700:Verapamil / Q000494:pharmacology*
D015062:11-Hydroxycorticosteroids; D000631:Aminopyridines; D015221:Potassium Channels; D000324:Adrenocorticotropic Hormone; D014700:Verapamil; C032439:alpha-aminopyridine
Vliianie blokatorov Ca2+- i K+-kanalov na AKTG-stimulirovannyĭ steroidogenez izolirovannykh nadpochechnikov vzroslykh i starykh krys.