title: Evidence that the adenylate cyclase secreted from Bordetella pertussis does not enter animal cells by receptor-mediated endocytosis.
abstract: Bordetella pertussis, the pathogen responsible for whooping cough, produces a calmodulin-sensitive adenylate cyclase. Several investigators have shown that the partially purified adenylate cyclase is capable of entering animal cells and elevating intracellular cAMP levels (Confer and Eaton: Science 217:948-950, 1982; Shattuck and Storm: Biochemistry 24:6323-6328, 1985). However, the mechanism for entry of the catalytic subunit of this adenylate cyclase into animal cells is unknown. It has been reported that the B. pertussis adenylate cyclase extracted from bacterial cells with urea does not enter animal cells by receptor-mediated endocytosis. There is, in addition to the cell associated form of the B. pertussis adenylate cyclase, a cell-invasive form of the enzyme secreted into the bacterial culture media. The properties of the cell-associated and secreted enzymes are significantly different (Masure and Storm: Biochemistry 28:438-442, 1989). In this study, we report evidence that the secreted form of the B. pertussis adenylate cyclase enters animal cells by a mechanism distinct from receptor-mediated endocytosis.
authors.affiliation: Department of Pharmacology, University of Washington, Seattle 98195.
authors.forename: M G
authors.initials: MG
authors.lastname: Donovan
authors.forename: D R
authors.initials: DR
authors.lastname: Storm
mesh_terms: D000262:Adenylyl Cyclases / Q000096:biosynthesis / Q000378:metabolism*; D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport; D001886:Bordetella pertussis / Q000201:enzymology*; D002460:Cell Line; D002738:Chloroquine / Q000494:pharmacology; D004705:Endocytosis / Q000187:drug effects; D007700:Kinetics; D051379:Mice; D008985:Monensin / Q000494:pharmacology; D009447:Neuroblastoma; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000378:metabolism; D013816:Thermodynamics
chemical_list: D011956:Receptors, Cell Surface; D000643:Ammonium Chloride; D002738:Chloroquine; D008985:Monensin; D000262:Adenylyl Cyclases