Fetal bovine serum inhibits chondrocyte collagenase production: interleukin 1 reverses this effect.
Bovine articular chondrocytes incubated in medium which was serum free or contained low levels of fetal bovine serum (less than 5%) constitutively produced collagenase. Increasing the concentration of serum in the culture medium inhibited the production of collagenase. Addition of interleukin 1 and lipopolysaccharide reversed the inhibitory effect of serum. Phorbol esters only stimulated collagenase production when the serum concentration was at least 10%. These data suggest that there is a factor(s) in fetal bovine serum that inhibits collagenase production by chondrocytes and this can be reversed by agents such as interleukin 1.
Kandel
R A
RA
Department of Pathology, Mount Sinai Hospital, Toronto, Canada.
Pritzker
K P
KP
Mills
G B
GB
Cruz
T F
TF
D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects / Q000201:enzymology*; D002417:Cattle; D002478:Cells, Cultured; D005333:Fetus; D007375:Interleukin-1 / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D003012:Microbial Collagenase / Q000096:biosynthesis*; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007375:Interleukin-1; D008070:Lipopolysaccharides; D012710:Serum Albumin, Bovine; D003012:Microbial Collagenase; D013755:Tetradecanoylphorbol Acetate