srd1, a Saccharomyces cerevisiae suppressor of the temperature-sensitive pre-rRNA processing defect of rrp1-1.
We define a new gene, SRD1, involved in the processing of pre-rRNA to mature rRNA. The SRD1 gene was identified by selecting for second-site suppressors of the previously described rrp1-1 mutation. The rrp1-1 mutation causes temperature-sensitive growth, a conditional defect in processing of 27S pre-rRNA to mature 25S rRNA, and a nonconditional increase in sensitivity to several aminoglycoside antibiotics. All srd1 alleles identified are recessive and apparently specific to the rrp1-1 mutation. Although a mutation of SRD1 suppresses the pre-rRNA processing defect, drug sensitivity and thermolethality of a point mutation of RRP1, it is unable to suppress a rrp1-disruption allele. We suggest that the SRD1 gene product either interacts with or regulates the RRP1 product.
Fabian
G R
GR
Department of Biological Chemistry, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033.
Hess
S M
SM
Hopper
A K
AK
D000483:Alleles; D001483:Base Sequence; D015139:Blotting, Southern; D003433:Crosses, Genetic; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D012322:RNA Precursors / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D012335:RNA, Ribosomal / Q000378:metabolism*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D013489:Suppression, Genetic; D013696:Temperature
D012322:RNA Precursors; D012335:RNA, Ribosomal