title: Nucleosome positioning modulates accessibility of regulatory proteins to the mouse mammary tumor virus promoter.
abstract: Minichromosomes containing the MMTV hormone responsive element (HRE) exhibit precisely positioned nucleosomes. Chromatin reconstitution of short HRE DNA fragments also results in precise positioning of nucleosomes as revealed by footprinting, which suggests that information for nucleosome phasing is contained within this short sequence. While hormone receptors bind naked DNA and reconstituted nucleosomes with similar affinities (3- to 5-fold difference), NFI, a transcription factor essential for efficient utilization of the MMTV promoter, binds naked DNA very tightly but does not bind the nucleosomally organized promoter. Hormone receptor binding to the MMTV nucleosome does not dissociate the nucleosome but leads to greater accessibility of the promoter-proximal end to exonuclease III. Precise positioning of one nucleosome over the MMTV promoter could repress transcription by preventing NFI binding in the absence of hormone, while still allowing interaction of activated hormone receptor with HRE.
authors.affiliation: Institut für Molekularbiologie und Tumorforschung, Marburg, Federal Republic of Germany.
authors.forename: B
authors.initials: B
authors.lastname: Piña
authors.forename: U
authors.initials: U
authors.lastname: Brüggemeier
authors.forename: M
authors.initials: M
authors.lastname: Beato
mesh_terms: D000818:Animals; D001483:Base Sequence; D002843:Chromatin / Q000502:physiology* / Q000648:ultrastructure; D004279:DNA, Viral / Q000235:genetics*; D003850:Deoxyribonuclease I; D005609:Free Radicals; D005809:Genes, Regulator; D006878:Hydroxides; D017665:Hydroxyl Radical; D008099:Liver / Q000502:physiology; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics*; D008961:Models, Structural; D008969:Molecular Sequence Data; D009707:Nucleosomes / Q000502:physiology* / Q000648:ultrastructure; D009710:Nucleotide Mapping; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: D002843:Chromatin; D004279:DNA, Viral; D005609:Free Radicals; D006878:Hydroxides; D009707:Nucleosomes; D011956:Receptors, Cell Surface; D017665:Hydroxyl Radical; D003850:Deoxyribonuclease I