Binding of [3H]cytochalasin B to tumoral islet cells.
Tumoral pancreatic islet cells of the RINm5F line are equipped with two classes of [3H]cytochalasin B binding sites with respective Kd of 0.4 and 7 microM. The binding of the fungal metabolite and its dissociation from the binding sites display rapid time courses. The binding is inhibited by D-glucose, more than by L-glucose, by phlorizin and by cytochalasin E. These findings are considered in the light of the dual action of cytochalasin B upon hexose transport and motile activity in islet cells.
Garcia-Mateu
S
S
Fundacion Jimenez Diaz, Centro Associado al Consejo Superior de Investigaciones Cientificas, Madrid, Spain.
Barreto
M
M
Valverde
I
I
Malaisse
W J
WJ
D007516:Adenoma, Islet Cell / Q000378:metabolism*; D001665:Binding Sites; D003571:Cytochalasin B / Q000378:metabolism*; D003572:Cytochalasins / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D007515:Islets of Langerhans / Q000378:metabolism*; D007700:Kinetics; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D010695:Phlorhizin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D003572:Cytochalasins; C007215:cytochalasin E; D003571:Cytochalasin B; D010695:Phlorhizin; D005947:Glucose