Chemically induced hepatic cytosol from the Sprague-Dawley rat: evidence for specific binding of 2,3,7,8-tetrachlorodibenzo-p-dioxin to components kinetically distinct from the Ah receptor.
A series of exogenous chemicals was used as potential inducers for the hepatic Ah receptor in the Sprague-Dawley rat. 2,3,7,8-Tetrachlorodibenzo-p-dioxin, 2,2',4,4',5,5'-hexachlorobiphenyl and phenobarbital all induced an elevated level of 3H-2,3,7,8-tetrachlorodibenzo-p-dioxin specific binding, while 3,3',4,4'-tetrachloroazobenzene and trans-3,3',4,4'-tetrachlorostilbene caused a depression. Mixtures of these chemicals caused additive effects. Elevated levels of specific binding appeared to be heterologous, comprising a binding species having the normal high stability of the Ah receptor in its liganded form, and another less stable substance having a half-life of approximately 2 h at 37 degrees C.
Landers
J P
JP
Guelph-Waterloo Centre for Graduate Work in Chemistry, Department of Chemistry and Biochemistry, University of Guelph, Ontario, Canada.
Birse
L M
LM
Nakai
J S
JS
Winhall
M J
MJ
Bunce
N J
NJ
D000818:Animals; D001391:Azo Compounds / Q000494:pharmacology; D002722:Chlorobenzenes / Q000494:pharmacology; D003600:Cytosol / Q000378:metabolism; D004147:Dioxins / Q000378:metabolism*; D006581:Hexachlorobenzene / Q000494:pharmacology; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010634:Phenobarbital / Q000494:pharmacology; D011078:Polychlorinated Biphenyls / Q000494:pharmacology; D000072317:Polychlorinated Dibenzodioxins / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D018336:Receptors, Aryl Hydrocarbon; D011955:Receptors, Drug / Q000187:drug effects / Q000378:metabolism*
D001391:Azo Compounds; D002722:Chlorobenzenes; D004147:Dioxins; D000072317:Polychlorinated Dibenzodioxins; D018336:Receptors, Aryl Hydrocarbon; D011955:Receptors, Drug; C013279:3,4,3',4'-tetrachloroazobenzene; D006581:Hexachlorobenzene; D011078:Polychlorinated Biphenyls; D010634:Phenobarbital