Isolation of a possible archetypal JC virus DNA sequence from nonimmunocompromised individuals.
We molecularly cloned JC polyomavirus DNAs from urine samples of eight nonimmunosuppressed patients and two healthy individuals. The cloned viral DNAs all contained an archetypal regulatory sequence from which various regulatory sequences of JC polyomavirus isolates derived from patients with progressive multifocal leukoencephalopathy could have evolved by deletion and amplification.
Yogo
Y
Y
Department of Viral Infection, University of Tokyo, Japan.
Kitamura
T
T
Sugimoto
C
C
Ueki
T
T
Aso
Y
Y
Hara
K
K
Taguchi
F
F
D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification / Q000652:urine*; D006801:Humans; D007108:Immune Tolerance; D007577:JC Virus / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010957:Plasmids; D011120:Polyomavirus / Q000302:isolation & purification*; D012016:Reference Values; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping
D004279:DNA, Viral