title: In vivo evaluation and comparison of collagen, acetylated collagen and collagen/glycosaminoglycan composite films and sponges as candidate biomaterials.
abstract: Native collagen, acetylated collagen, collagen/10% chondroitin sulphate, collagen/2.5% hyaluronic acid and collagen/20% hyaluronic acid were implanted both as film and as sponge into rat lumbar muscle for 7 and 14 d. After 7 d implantation, all materials elicited an acute inflammatory cell response characterized by numerous polymorphs and histocytes. The cell population after 14 d was principally mononuclear, i.e. leucocytes, neutrophils, macrophages, lymphocytes and fibroblasts. Both films and sponges followed a similar pattern. Native collagen elicited a subacute inflammatory response after 7 d. However, 14 d after implantation, a marked infiltration by neutrophils was apparent with subsequent degradation of existing collagen material. Acetylated collagen film evoked a much greater inflammatory cell response than native collagen. Both collagen/hyaluronic acid composites elicited a similar response. The collagen/10% chondroitin sulphate composite elicited the least inflammatory cell response at 7 d, whereas infiltration by host fibroblasts after 14 d implantation was clearly seen.
authors.affiliation: Devro Ltd, Moodiesburn, Chryston, Glasgow, UK.
authors.forename: S
authors.initials: S
authors.lastname: Srivastava
authors.forename: S D
authors.initials: SD
authors.lastname: Gorham
authors.forename: D A
authors.initials: DA
authors.lastname: French
authors.forename: A A
authors.initials: AA
authors.lastname: Shivas
authors.forename: J M
authors.initials: JM
authors.lastname: Courtney
mesh_terms: D000107:Acetylation; D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects; D003094:Collagen / Q000378:metabolism / Q000633:toxicity; D006025:Glycosaminoglycans / Q000378:metabolism / Q000633:toxicity; D006820:Hyaluronic Acid / Q000032:analysis; D007249:Inflammation / Q000209:etiology; D008422:Materials Testing; D003012:Microbial Collagenase / Q000378:metabolism; D009132:Muscles / Q000601:surgery; D011108:Polymers / Q000378:metabolism / Q000633:toxicity; D051381:Rats; D015503:Surgical Sponges / Q000009:adverse effects
chemical_list: D001672:Biocompatible Materials; D006025:Glycosaminoglycans; D011108:Polymers; C058506:collagen-glycosaminoglycan copolymer; D006820:Hyaluronic Acid; D003094:Collagen; D003012:Microbial Collagenase