title: Involvement of vitamin D3 with cardiovascular function. III. Effects on physical and morphological properties.
abstract: We have previously shown that depletion of vitamin D3 in rats results in a large increase in the contractile function of isolated hearts (R. E. Weishaar, J. Clin. Invest. 79: 1706-1712, 1987). To characterize the mechanism responsible for this increase, the effect of vitamin D3 depletion on key physical and morphological properties of cardiac muscle was examined. Depletion of vitamin D3 increased the heart weight/body weight ratio. This increase could neither be blocked by limiting hypocalcemia nor reversed by restoring increasing serum calcium levels. The cardiomegaly observed 9 wk after vitamin D3 depletion was not accompanied by an increase in myocardial water content or leakage of myocardial creatine phosphokinase and was not caused by myocardial cell hypertrophy. Histological examination of ventricular muscle from vitamin D3-deficient rats revealed a significant decrease in myofibrillar area and a significant increase in extracellular space. The increase in extracellular space was accompanied by a significant increase in myocardial collagen. Prevention of hypocalcemia in the vitamin D3-deficient rats did not prevent the increase in myocardial collagen. Such alterations in the physical and morphological properties of myocardial tissue might represent the basis for the change in myocardial contractile function that accompanies lengthy periods of vitamin D3 deficiency.
authors.affiliation: Department of Pharmacology, Parke-Davis Pharmaceutical, Warner-Lambert Company, Ann Arbor 48109.
authors.forename: R E
authors.initials: RE
authors.lastname: Weishaar
authors.forename: S N
authors.initials: SN
authors.lastname: Kim
authors.forename: D E
authors.initials: DE
authors.lastname: Saunders
authors.forename: R U
authors.initials: RU
authors.lastname: Simpson
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002118:Calcium / Q000097:blood; D002395:Catecholamines / Q000097:blood; D002762:Cholecalciferol / Q000494:pharmacology*; D003402:Creatine Kinase / Q000378:metabolism; D005110:Extracellular Space; D006321:Heart / Q000033:anatomy & histology* / Q000187:drug effects; D006728:Hormones / Q000097:blood; D008274:Magnesium / Q000097:blood; D008297:Male; D008854:Microscopy, Electron; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000201:enzymology / Q000473:pathology / Q000648:ultrastructure; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D014808:Vitamin D Deficiency / Q000503:physiopathology*
chemical_list: D002395:Catecholamines; D006728:Hormones; D002762:Cholecalciferol; D003402:Creatine Kinase; D008274:Magnesium; D002118:Calcium