Spontaneous induction of malignancy in mouse cells by a human small cell lung cancer implanted in nude mice.
This report provides evidence for spontaneous transfer of human tumor DNA in vivo to mouse cells by a human small cell lung cancer implanted in nude mice. The transformation of the mouse cells was characterized by the presence of neurosecretory granules, which are hallmarks of small cell lung cancer. The carcinogenicity of the human xenografted tumor DNA was confirmed by transfecting NIH3T3 cells in vitro, suggesting that the human tumor DNA may have been the cause of the transformation of the mouse cells in vivo. The spontaneous induction of malignancy in mouse cells in vivo by human tumor DNA may be a factor of potential importance in tumor cell heterogeneity and propagation of the malignant state for some tumors. The observations support the thesis that some human cancer cells can transform normal cells to become malignant in vivo.
Gupta
V
V
Division of Medical Oncology, Pittsburgh Cancer Institute, PA.
Rajaraman
S
S
Eberle
R
R
D000818:Animals; D018288:Carcinoma, Small Cell / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000473:pathology; D002871:Chromosome Banding; D004273:DNA, Neoplasm / Q000235:genetics; D005784:Gene Amplification; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D007527:Isoenzymes / Q000032:analysis; D007621:Karyotyping; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008175:Lung Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D008291:Malate Dehydrogenase / Q000032:analysis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009693:Nucleic Acid Hybridization; D010042:Ouabain / Q000494:pharmacology; D011683:Purine-Nucleoside Phosphorylase / Q000032:analysis; D012474:Salmon; D014162:Transfection; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D007527:Isoenzymes; D010042:Ouabain; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D011683:Purine-Nucleoside Phosphorylase