The inhibitory glycine receptor: a ligand-gated chloride channel of the central nervous system.
The postsynaptic glycine receptor (GlyR) is a major inhibitory chloride channel protein in the central nervous system. The affinity-purified receptor contains polypeptides of 48 kDa, 58 kDa, and 93 kDa. The 48-kDa (alpha) and 58 kDa (beta) subunits span the postsynaptic membrane in a pentameric arrangement to form the anion channel of the receptor. The 93-kDa polypeptide is cytoplasmically localized and may have an anchoring function. Molecular cloning revealed that different structural characteristics are shared by the membrane-spanning subunits of the GlyR and those of other ligand-gated ion channel proteins. Developmental regulation of the GlyR is characterized by alterations in antagonist binding, heterogeneity of alpha subunits, and increased levels of the 93-kDa polypeptide. Glycine receptor function can be reconstituted by expression of cloned alpha subunits in heterologous cell systems. Positive charges found at the presumed mouths of the GlyR channel appear to be important determinants of ion selectivity. These data establish the anion-conducting GlyR as a homolog of other ligand-gated ion channel proteins and suggest that the diversity of these channels originates from divergent evolution of a primordial channel protein early in phylogeny.
Langosch
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Zentrum für Molekulare Biologie, Universität Heidelberg, Federal Republic of Germany.
Becker
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CM
Betz
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H
D000595:Amino Acid Sequence; D000818:Animals; D018118:Chloride Channels; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins / Q000502:physiology; D008565:Membrane Proteins / Q000502:physiology*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter / Q000145:classification / Q000502:physiology*; D013329:Structure-Activity Relationship
D018118:Chloride Channels; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter