Receptors for atrial natriuretic peptide (ANP) and cyclic GMP responses in HeLa cells.
Cultured HeLa cells display specific binding sites for atrial natriuretic peptide (ANP). Studies with [125I]-(1-28)-rat ANP revealed the presence of a single class of high affinity binding sites on HeLa cells: The apparent dissociation constant (Kd) was 5 nM and the maximal number was 29,000 sites/cell as derived from Scatchard analysis. Furthermore, ANP elevates levels of cGMP in a dose dependent manner with an EC50 close to the Kd for [125I]ANP binding. ANP-mediated elevation of cellular cGMP leads to a significant reduction of bumetanide-sensitive Na+, K+, 2Cl(-)-cotransport in HeLa cells, studied by 22Na+ and 86Rb+ influx measurements. These data indicate (1) that distinct functionally active receptors for ANP are present on HeLa cells and (2) ANP influences the activity of Na+,K+,2Cl(-)-cotransport in these cells.
Kort
J J
JJ
Department of Molecular Biology, University of Hamburg, FRG.
Koch
G
G
D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D001692:Biological Transport; D002712:Chlorides / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism*; D005260:Female; D006367:HeLa Cells / Q000502:physiology*; D006801:Humans; D011188:Potassium / Q000378:metabolism; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000502:physiology*; D012413:Rubidium / Q000378:metabolism; D015398:Signal Transduction; D012964:Sodium / Q000378:metabolism; D013997:Time Factors
D002712:Chlorides; D011956:Receptors, Cell Surface; D009320:Atrial Natriuretic Factor; D012964:Sodium; D017461:Receptors, Atrial Natriuretic Factor; D006152:Cyclic GMP; D012413:Rubidium; D011188:Potassium