Cholesterol movement between the plasma membrane and the cholesteryl ester droplets of cultured Leydig tumour cells.
The present studies characterize the turnover of plasma membrane cholesterol in MA-10 Leydig tumour cells. Plasma membrane cholesterol of MA-10 cells was slowly internalized and converted into cholesteryl ester. Low-density lipoprotein (LDL) stimulated, in a dose- and time-dependent fashion, plasma membrane cholesterol conversion into intracellular esters. Stimulation of membrane internalization was not simply the consequence of accelerated uptake of membrane with LDL, since binding and internalization of epidermal growth factor and transferrin had no effect on turnover of plasma membrane cholesterol. The protein of LDL is unimportant as well, since delipidated LDL had no effect on membrane turnover. The action of LDL on cholesterol turnover was explained entirely by its contribution to cholesteryl ester stores. The degree of plasma membrane cholesterol internalization and esterification was directly proportional to the size of cellular ester stores.
Nagy
L
L
Division of Endocrinology, Veterans Administration Medical Center, Dallas, TX.
Freeman
D A
DA
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism*; D002788:Cholesterol Esters / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007424:Intracellular Fluid / Q000378:metabolism; D007504:Iron Radioisotopes; D007984:Leydig Cell Tumor / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D008077:Lipoproteins, LDL / Q000494:pharmacology; D051379:Mice; D014168:Transferrin / Q000378:metabolism
D002788:Cholesterol Esters; D007504:Iron Radioisotopes; D008077:Lipoproteins, LDL; D014168:Transferrin; D004815:Epidermal Growth Factor; D003994:Bucladesine; D002784:Cholesterol