Molecular structure of frizzled, a Drosophila tissue polarity gene.
The function of the frizzled (fz) locus is required to coordinate the cytoskeletons of pupal epidermal cells so that a parallel array of cuticular hairs and bristles is produced. We report here the molecular cloning and characterization of the fz locus. The locus is very large. Mutations that inactivate the gene are spread over 100 kb of genomic DNA. The major mRNA product of the gene is a 4-kb RNA that is encoded by 5 exons spread over more than 90 kb of genomic DNA. Conceptual translation of this mRNA indicates that it encodes an integral membrane protein that is likely to contain both extracellular and cytoplasmic domains.
Adler
P N
PN
Biology Department, University of Virginia, Charlottesville 22901.
Vinson
C
C
Park
W J
WJ
Conover
S
S
Klein
L
L
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000235:genetics*; D005260:Female; D051157:Frizzled Receptors; D005796:Genes; D007301:Insect Hormones / Q000235:genetics*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D043562:Receptors, G-Protein-Coupled; D015183:Restriction Mapping
D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D051157:Frizzled Receptors; D007301:Insect Hormones; D008565:Membrane Proteins; D043562:Receptors, G-Protein-Coupled; C058784:fz protein, Drosophila