Expression of the Cellulomonas fimi cellulase genes cex and cenA from the divergent tet promoters of transposon Tn10.
A cartridge was constructed which contained the divergent tet promoters of transposon Tn10 between an exoglucanase gene (cex) and an endoglucanase gene (cenA) of Cellulomonas fimi. When carried in a broad-host-range vector, the cartridge gave expression of cex and cenA in Escherichia coli, Rhodobacter capsulatus and Klebsiella pneumoniae.
Din
N
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Department of Microbiology, University of British Columbia, Vancouver, Canada.
Beck
C F
CF
Miller
R C
RC
Kilburn
D G
DG
Warren
R A
RA
D000192:Actinomycetales / Q000201:enzymology / Q000235:genetics*; D002480:Cellulase / Q000235:genetics*; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D007711:Klebsiella pneumoniae / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012244:Rhodospirillaceae / Q000235:genetics
D004251:DNA Transposable Elements; D002480:Cellulase