title: Probes for vasopressin receptors. Attachment of affinity and fluorescent groups in vasopressin.
abstract: Fluorescent, photoreactive, and biotinylated analogs of vasopressin have been prepared in which one of these three groups has been attached to a reactive amino group in either position 4 or position 7. Using solid phase methodology, we have synthesized two active parent compounds, [1-desamino,4-lysine,7-hydroxyproline]arginine vasopressin and [1-desamino,7-aminoproline]arginine vasopressin, and acylated them to obtain biotinyl, azidobenzoyl, and fluoresceinyl derivatives. We have also prepared analogs in which a "spacer arm" was inserted between lysine in position 4 and the marker group. Some of these derivatives have good antidiuretic activity and could be valuable probes in studying hormone-receptor interaction and in receptor visualization and purification.
authors.affiliation: Department of Physiology and Biophysics, Mount Sinai School of Medicine of CUNY, NY.
authors.forename: A
authors.initials: A
authors.lastname: Buku
authors.forename: D
authors.initials: D
authors.lastname: Gazis
mesh_terms: D000345:Affinity Labels; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005453:Fluorescence; D006909:Hydroxyproline; D008239:Lysine; D013058:Mass Spectrometry; D011956:Receptors, Cell Surface; D014667:Vasopressins
chemical_list: D000345:Affinity Labels; D011956:Receptors, Cell Surface; D014667:Vasopressins; D008239:Lysine; D006909:Hydroxyproline