Effect of acyclovir on herpes simplex virus infected neuronal, glial, and neurohybridoma cells in vitro.
Herpesvirus type 1 could be propagated most efficiently in cultured fetal neurons, to a lesser extent in NG108-15 neurohybridoma cells, and with the lowest titer in glial cells. Herpesvirus type 2 could not be cultured in neurohybridoma cells, and in fetal neurons a titer 100-fold lower than for herpesvirus type 1 was obtained. Cells infected with herpesvirus type 1 were used in an infectivity assay for acyclovir dose-response studies. The ED50 values were 7.4 nmol/l for fetal neurons, 180 for neurohybridoma cells, and 275 nmol/l for glial cells.
Borcholte
T
T
Deutsche Wellcome GmbH, Burgwedel, FRG.
Kliem
V
V
Verhagen
W
W
Wellhöner
H H
HH
D000212:Acyclovir / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D006825:Hybridomas; D051379:Mice; D009457:Neuroglia / Q000382:microbiology*; D009474:Neurons / Q000382:microbiology*; D018139:Simplexvirus / Q000187:drug effects* / Q000254:growth & development; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology; D014776:Virus Cultivation
D000212:Acyclovir