The neutrophil and chronic allergic inflammation. Immunochemical localization of neutrophil elastase.
To test whether neutrophils infiltrate and degranulate in areas of chronic respiratory allergic inflammation, we developed an indirect immunofluorescence technique to localize neutrophil elastase in formalin-fixed, paraffin-embedded tissues. The affinity-purified antielastase stained only neutrophils on peripheral blood buffy coat smears, and in lung tissue from patients with pneumonia. We examined tissue specimens from four patients with fatal asthma, 10 patients with chronic sinusitis, and 10 patients with nasal polyposis for the presence of elastase, as well as eosinophil granule major basic protein (MBP). Neutrophil infiltration and extracellular elastase deposition in association with damage to respiratory epithelium were generally sparse in most specimens; the exceptions were one patient with asthma, one patient with chronic sinusitis, and two patients with nasal polyposis. In contrast, eosinophil infiltration and extracellular MBP deposition were generally marked in most specimens; the exceptions were one patient with asthma and one patient with nasal polyps where extracellular MBP deposition did not coincide with damage to respiratory epithelium. The results suggest that the neutrophil does not usually infiltrate tissues showing allergic inflammation; however, on occasion, it may participate in these inflammatory reactions.
Fujisawa
T
T
Allergic Diseases Research Laboratory, Mayo Clinic, Rochester, MN 55905.
Kephart
G M
GM
Gray
B H
BH
Gleich
G J
GJ
D000328:Adult; D000368:Aged; D001249:Asthma / Q000378:metabolism / Q000473:pathology; D001798:Blood Proteins / Q000032:analysis; D002908:Chronic Disease; D003594:Cytoplasmic Granules / Q000473:pathology; D047091:Eosinophil Granule Proteins; D004804:Eosinophils / Q000032:analysis / Q000473:pathology; D004848:Epithelium / Q000032:analysis / Q000473:pathology; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007249:Inflammation / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D008168:Lung / Q000032:analysis / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000378:metabolism / Q000473:pathology; D009504:Neutrophils / Q000201:enzymology* / Q000473:pathology; D010196:Pancreatic Elastase / Q000032:analysis*; D012130:Respiratory Hypersensitivity / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D012260:Ribonucleases; D012852:Sinusitis / Q000378:metabolism / Q000473:pathology
D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D012260:Ribonucleases; D010196:Pancreatic Elastase