Immunohistochemical demonstration of fibre type-specific isozymes of cytochrome c oxidase in human skeletal muscle.
The immunohistochemical reaction of monoclonal as well as polyclonal antibodies against cytochrome c oxidase (COX) subunits with serial sections of normal human skeletal muscle was investigated. The stronger reactivity of polyclonal antibodies to COX subunits II-III and VIIbc with type I as compared to type II fibres, correlated well with the higher histochemical reactivity of NADH dehydrogenase, succinate dehydrogenase and cytochrome c oxidase in type I fibres. In contrast an almost exclusive reaction of a monoclonal antibody against subunit IV with type I fibre and a preponderant reaction of a polyclonal antibody against subunits Vab with type II fibres was obtained. Antibodies against subunits I, Vb and VIc did not reveal a fibre-type-specific reactivity. The data indicate in human muscle the occurrence of fibre type-specific isozymes of cytochrome c oxidase differing in subunits IV and Va or Vb.
Romero
N
N
Unite de Recherche Biologie et Pathologie Neuromusculaires INSERM U 153, CNRS UA 614, Paris, France.
Marsac
C
C
Fardeau
M
M
Droste
M
M
Schneyder
B
B
Kadenbach
B
B
D000911:Antibodies, Monoclonal; D003576:Electron Transport Complex IV / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism*; D046911:Macromolecular Substances; D009132:Muscles / Q000166:cytology / Q000201:enzymology*
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D046911:Macromolecular Substances; D003576:Electron Transport Complex IV