Transfer of iron from uteroferrin (purple acid phosphatase) to transferrin related to acid phosphatase activity.
There is continuing controversy as to whether iron can be exchanged from the purple phosphatase, uteroferrin (Uf), to fetal transferrin (Tf) and whether this process might be of physiological relevance during pregnancy in the pig. Here, iron transfer from Uf to apoTf at pH 7.1 was followed by measuring the loss of acid phosphatase activity from native Uf as a function of incubation conditions and time. In the presence of apoTf and 1 mM ascorbate (but not in the presence of either agent alone), 50% of enzyme activity was lost in about 12 h. Loss of activity was accompanied by bleaching of Uf purple color and the appearance of the characteristic visual absorption spectrum of Fe-Tf. Citrate could replace ascorbate in the reaction. Loss of Uf iron did not occur at pH 5.3, at which pH Tf cannot bind Fe. [59Fe]Uf was prepared and shown to be identical in its enzymatic and physical properties with unmodified Uf. Transfer of 59Fe from Uf to apo-Tf was promoted by conditions identical to those which led to loss of purple color and acid phosphatase activity. However, the results suggested that only one of the two iron atoms at the bi-iron center on Uf was readily lost, and that exchange of the second iron occurred more slowly. Loss of iron made Uf more susceptible to denaturation. A third technique, quantitation of the g' = 4.3 signal of iron specifically bound to Tf by EPR, was also tested as a means assaying accumulation of Fe-Tf, but the method was too insensitive to measure the kinetics of iron transfer at physiological protein concentrations. We conclude that iron can be transferred directly from Uf to apoTf in the presence of low molecular weight chelators, and that the process is likely to be of physiological significance.
Nuttleman
P R
PR
Department of Animal Sciences, University of Missouri, Columbia 65211.
Roberts
R M
RM
D000135:Acid Phosphatase / Q000378:metabolism; D000482:Allantois / Q000032:analysis; D000818:Animals; D001059:Apoproteins / Q000378:metabolism; D001205:Ascorbic Acid / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D066298:In Vitro Techniques; D007501:Iron / Q000378:metabolism*; D007527:Isoenzymes; D007700:Kinetics; D008667:Metalloproteins / Q000378:metabolism*; D013057:Spectrum Analysis; D013552:Swine; D000071681:Tartrate-Resistant Acid Phosphatase; D014168:Transferrin / Q000378:metabolism*
D001059:Apoproteins; D007527:Isoenzymes; D008667:Metalloproteins; D014168:Transferrin; C028284:apotransferrin; D007501:Iron; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D001205:Ascorbic Acid