Alternative splicing gives rise to two forms of the p68 Ca2(+)-binding protein.
The p68 Ca2+ and phospholipid binding protein of the lipocortin/calpactin family appears to exist as two forms. These may be resolved into a closely-spaced polypeptide doublet by SDS-PAGE. The cloning and sequencing of p68 revealed an apparent 18 nucleotide alternative splice sequence, which could account for this observation. We show here that an antiserum directed against a synthetic peptide corresponding to the region containing the splice sequence, recognises only the upper band of the p68 doublet by both immunoprecipitation and Western blotting. These results are consistent with alternative splicing being responsible for the generation of the two forms of p68.
Moss
S E
SE
Cell Surface Biochemistry Laboratory, Imperial Cancer Research Fund, London, England.
Crumpton
M J
MJ
D000595:Amino Acid Sequence; D000818:Animals; D017317:Annexin A6; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D007163:Immunosorbent Techniques; D015458:Leukemia, T-Cell; D008969:Molecular Sequence Data; D012326:RNA Splicing; D051381:Rats; D014407:Tumor Cells, Cultured
D017317:Annexin A6; D002135:Calcium-Binding Proteins