Analysis of the role of the extracellular domain of the v-erbB oncogene in cellular transformation.
The role of the extracellular domain of the v-erbB oncogene in the transformation of chicken fibroblasts and erythroblasts has been examined. A virus was isolated in which the complete extracellular domain and the transmembrane domain of the v-erbB were replaced with sequences from the gag and env genes. The resulting virus, GEE35, was capable of transforming both fibroblasts and erythroblasts as efficiently as wild-type v-erbB. Biochemical and immunochemical analysis of the v-erbB protein encoded by GEE35 revealed two proteins of apparent molecular weights of approximately 130,000 daltons. One of these proteins was an N-linked glycosylated membrane protein, whereas the other was cytoplasmic. The possible roles of either of these two proteins in transformation is discussed.
Lee
E B
EB
Department of Microbiology, State University of New York, Stony Brook 11794.
Hayman
M J
MJ
D001355:Alpharetrovirus / Q000235:genetics*; D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics*; D002471:Cell Transformation, Neoplastic; D002642:Chick Embryo; D015683:Gene Products, gag / Q000032:analysis; D008562:Membrane Glycoproteins / Q000032:analysis; D009856:Oncogene Proteins, Viral / Q000032:analysis / Q000502:physiology; D009857:Oncogenes; D010766:Phosphorylation; D010957:Plasmids; D014443:Tyrosine / Q000378:metabolism
D015683:Gene Products, gag; D008562:Membrane Glycoproteins; D009856:Oncogene Proteins, Viral; D014443:Tyrosine