Cell lines derived from dorsal root ganglion neurons are nonpermissive for HSV and express only the latency-associated transcript following infection.
The effect of herpes simplex virus type 1 infection in a series of immortalized dorsal root ganglion cell lines has been investigated. Following infection of one of these lines, the viral immediate-early genes are not transcribed and the lytic cycle is aborted at an early stage. In contrast these cells do support transcription of the gene encoding the latency-associated transcripts which are the only viral RNAs present in latently infected ganglia in vivo. These cell lines are therefore a suitable model system for studies of the processes regulating the interaction of HSV with neuronal cell types and the establishment of latent infections in vivo.
Wheatley
S C
SC
Department of Biochemistry, University College and Middlesex School of Medicine, London, United Kingdom.
Kemp
L M
LM
Wood
J N
JN
Latchman
D S
DS
D000818:Animals; D002460:Cell Line; D005727:Ganglia, Spinal / Q000166:cytology* / Q000382:microbiology; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral / Q000235:genetics; D006561:Herpes Simplex / Q000235:genetics*; D009475:Neurons, Afferent / Q000166:cytology* / Q000382:microbiology; D051381:Rats; D018139:Simplexvirus / Q000235:genetics* / Q000302:isolation & purification; D014158:Transcription, Genetic / Q000235:genetics*