Detection of antibodies to cytomegalovirus by immunofluorescence and enzyme immunoassay.
Immunofluorescence assay (IFA) and enzyme immunoassay (EIA) for detection of immunoglobulin M (IgM) to cytomegalovirus (CMV) correlated in 88.9%. All but one differences were due to negativity in IFA and positivity in EIA. The agreement between determination of immunoglobulin G (IgG) to CMV by IFA and determination of total antibodies to CMV by EIA was 89.9%. All the differences were due to a mild positivity in IFA and negativity in EIA. The advantage of EIA is in its objective colorimetric evaluation of test results excluding a subjective underestimation or overestimation of the reaction. In addition, EIA gives the result of IgM detection already in 6 hours, while IFA as late as in 24 hours.
Korcáková
L
L
Institute for Clinical and Experimental Medicine, Prague.
Kaslík
J
J
Slízová
I
I
D000914:Antibodies, Viral / Q000032:analysis*; D003587:Cytomegalovirus / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M