title: Insulin-like growth factor receptors in chicken liver membranes: binding properties, specificity, developmental pattern and evidence for a single receptor type.
abstract: Two distinct receptors for the insulin-like growth factors (IGF-I and IGF-II) have been identified in mammalian tissues, but so far only a receptor structurally related to the type I receptor has been identified in chicken embryonic tissues. This study was designed to characterize binding sites for IGF peptides in chicken liver microsomal membranes prepared from hatch to 10 weeks of age which is the period of most rapid growth. Binding of both human (h) IGF-I and hIGF-II was displaceable by either peptide and exhibited similar pH, time and temperature dependency. Human IGF-II was more potent than hIGF-I in competing for the binding of the iodinated ligands with half-maximum effective concentrations of 3-5 micrograms/l and 7-13 micrograms/l respectively. Porcine insulin was also a potent competitor. Affinity cross-linking studies, followed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis under reducing conditions demonstrated that both IGF peptides were linked to a protein with a molecular weight of about 130,000 Da characteristic of the alpha-subunit of the type I receptor. There was no evidence for the presence of a type II receptor similar to that found in mammals. Specific binding of both peptides was low on the day of hatch, increased about threefold by day 3 of age and remained high for the first 3 weeks of life before returning to a lower steady state level up to 10 weeks of age.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Molecular Endocrinology, AFRC Institute of Animal Physiology and Genetics Research, Edinburgh Research Station, Roslin, Midlothian.
authors.forename: M J
authors.initials: MJ
authors.lastname: Duclos
authors.forename: C
authors.initials: C
authors.lastname: Goddard
mesh_terms: D000375:Aging / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002645:Chickens / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007335:Insulin-Like Growth Factor II / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism*; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017451:Receptors, Somatomedin; D011994:Recombinant Proteins / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
chemical_list: D011956:Receptors, Cell Surface; D017451:Receptors, Somatomedin; D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II