Sequence comparison of five polymerases (L proteins) of unsegmented negative-strand RNA viruses: theoretical assignment of functional domains.
The large (L) protein subunit of unsegmented negative-strand RNA virus polymerases is thought to be responsible for the majority of enzymic activities involved in viral transcription and replication. In order to gain insight into this multifunctional role we compared the deduced amino acid sequences of five L proteins of rhabdoviruses (vesicular stomatitis virus and rabies virus) or paramyxoviruses (Sendai virus, Newcastle disease virus and measles virus). Statistical analysis showed that they share an atypical amino acid usage, outlining the uniqueness of the negative-strand virus life style. Similarity studies between L proteins traced evolutionary relationships in partial disagreement with the present taxonomic arrangement of this group of viruses. The five L proteins exhibit a high degree of homology along most of their length, with strongly invariant amino acids embedded in conserved blocks separated by variable regions, suggesting a structure of concatenated functional domains. The most highly conserved central block contains the probable active site for RNA synthesis. We tentatively identified some other functional sites, distributed around this central core, that would naturally work together to assure the polymerase activity. This provides detailed guidelines for the future study of L proteins by site-directed mutagenesis.
Poch
O
O
Groupe Biochimie II, Institut de Biologie Moléculaire et Cellulaire du C.N.R.S., Strasbourg, France.
Blumberg
B M
BM
Bougueleret
L
L
Tordo
N
N
D000595:Amino Acid Sequence; D005075:Biological Evolution; D012321:DNA-Directed RNA Polymerases; D007256:Information Systems; D008459:Measles virus / Q000201:enzymology; D008969:Molecular Sequence Data; D009522:Newcastle disease virus / Q000201:enzymology; D010222:Parainfluenza Virus 1, Human / Q000201:enzymology; D010252:Paramyxoviridae / Q000201:enzymology*; D011820:Rabies virus / Q000201:enzymology; D012209:Rhabdoviridae / Q000201:enzymology*; D012689:Sequence Homology, Nucleic Acid; D014721:Vesicular stomatitis Indiana virus / Q000201:enzymology
D012321:DNA-Directed RNA Polymerases