Effect of a high Na+ diet on cell volume and Na(+)-stimulated ATPase activities of rat kidney membranes.
Proximal tubular cells from kidneys of male rats chronically fed with an isotonic NaCl solution, show a volume increase which is dependent on the length of the treatment with NaCl, when compared with control rats. Parallel to the cell volume increase, there is an increase of the ouabain-insensitive Na-ATPase activity, whereas the ouabain-sensitive Na,K-ATPase activity remains unchanged. These results establish a clear relationship between a chronic Na-diet, kidney cell volume and Na-ATPase activity.
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Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigacions Científicas (IVIC), Caracas, Venezuela.
Henríquez
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LM
Proverbio
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Marín
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R
Proverbio
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D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D027682:Cation Transport Proteins; D066298:In Vitro Techniques; D007668:Kidney / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D007700:Kinetics; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012982:Sodium, Dietary / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D027682:Cation Transport Proteins; D012982:Sodium, Dietary; D000251:Adenosine Triphosphatases; C021838:sodium-translocating ATPase; D000254:Sodium-Potassium-Exchanging ATPase