Antiviral activity and pharmacokinetics of HOE 602, an acyclic nucleoside, in animal models.
The acyclic nucleoside derivative HOE 602 (2-amino-9-[1,3-bis(isopropoxy)-2-propoxymethyl]purine) was evaluated for its antiviral activity in cell culture and for its therapeutic efficacy in mice infected with herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) or with murine cytomegalovirus (MCMV). HOE 602 was inactive in vitro against a variety of DNA- and RNA-viruses. However it prevented symptoms and mortality in mice systemically infected with HSV-1, HSV-2 or MCMV when administered intraperitoneally or orally at a dosage of 100 mumol/kg twice per day. Pharmacokinetic studies in mice and macaques revealed that HOE 602 was converted via three metabolic steps to ganciclovir, which seemed to be the antivirally active compound. The bioavailability of ganciclovir after oral administration of HOE 602 or ganciclovir was similar in mice, while in rhesus monkeys much higher serum levels of ganciclovir were reached with HOE 602. After intraperitoneal or intravenous administration higher drug levels were obtained with ganciclovir. The excellent therapeutic efficacy in animal models, the high enteral absorption in monkeys, and the favourable physical properties will hopefully lead to an orally active drug against cytomegalovirus and severe herpes infections in man.
Winkler
I
I
Hoechst AG, Frankfurt, F.R.G.
Winkelmann
E
E
Scholl
T
T
Rösner
M
M
Jähne
G
G
Helsberg
M
M
D000212:Acyclovir / Q000494:pharmacology; D000818:Animals; D000998:Antiviral Agents / Q000493:pharmacokinetics / Q000494:pharmacology*; D001682:Biological Availability; D003586:Cytomegalovirus Infections / Q000188:drug therapy*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D015774:Ganciclovir / Q000493:pharmacokinetics / Q000494:pharmacology; D006367:HeLa Cells; D006561:Herpes Simplex / Q000188:drug therapy*; D008253:Macaca mulatta; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D011687:Purines / Q000493:pharmacokinetics / Q000494:pharmacology*; D014709:Vero Cells
D000998:Antiviral Agents; D011687:Purines; C066917:HOE 602; D015774:Ganciclovir; D000212:Acyclovir