Isolation of bovine eosinophils and characterization of their leukotriene formation.
A method for the isolation of eosinophils from bovine blood is described. Eosinophils were prepurified in a simple centrifugation step and subsequently separated on a Percoll gradient into two populations with densities of 1.088 and 1095 g/cm3, respectively, and a purity of greater than 90%. No apparent differences could be observed in electron micrographs and most of the granules appeared homogenous. A minor fraction of granules appeared to have a lamellated crystalline core structure. Eosinophils of bovine blood generate leukotriene B4 and its stereoisomers but not sulfidopeptide leukotrienes (slow-reacting substance) when stimulated by the Ca-ionophore A23187.
Freiburghaus
J
J
Institute of Biochemistry, University of Fribourg, Switzerland.
Jörg
A
A
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002417:Cattle; D002469:Cell Separation; D002851:Chromatography, High Pressure Liquid; D004804:Eosinophils / Q000166:cytology / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D017999:Leukotriene E4; D015289:Leukotrienes / Q000096:biosynthesis*; D013189:SRS-A / Q000031:analogs & derivatives / Q000096:biosynthesis; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D007202:Indicators and Reagents; D015289:Leukotrienes; D013189:SRS-A; D000001:Calcimycin; D017999:Leukotriene E4