[Preparation on monoclonal antibodies against laminin receptors and their inhibitory effects on attachment and spreading of tumor cells].
Monoclonal antibodies against laminin receptors (LN) isolated from a highly metastatic human giant cell carcinoma of lung (PG) were prepared by routine hybridoma technique. Two positive clones secreted monoclonal antibodies against laminin receptors, one of which was named McB1. McB1 was bound more efficiently to LN-R of cultured PG tumor cells immunohistochemically. McB1 could markedly inhibit the attachment and spreading of S-180 mouse ascitic tumor cells and PG tumor cells on laminin substrate. Monoclonal antibody against LN-R is definitely useful in studying the interaction between tumor cells and extracellular matrix and the molecular mechanisms of invasion and metastases of tumor cells.
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D002277:Carcinoma / Q000473:pathology; D002448:Cell Adhesion; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D011971:Receptors, Immunologic / Q000276:immunology*; D017474:Receptors, Laminin; D012510:Sarcoma 180 / Q000473:pathology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D011971:Receptors, Immunologic; D017474:Receptors, Laminin