Inhibitory effects of ginsenoside Rg1 and Rb1 on rat brain microsomal Na+,K(+)-ATPase activity.
Rat brain microsomal Na+, K(+)-ATPase activity was inhibited significantly and rapidly by ginsenoside Rb1. The IC50 of Rb1 for Na+,K(+)-ATPase was 6.3 +/- 1.0 mumol/L. The inhibition was enhanced with increasing the concentration of Rb1 or decreasing that of Na+ and K+. Kinetic analysis revealed that ginsenoside was an uncompetitive inhibitor with respect to ATP. The inhibitory effect of Rg1 on rat brain microsomal Na+,K(+)-ATPase was much weaker than that of Rb1.
Cao
J
J
Department of Pharmacology, Nanjing Medical College, China.
Zheng
Y Q
YQ
Liu
T P
TP
Feng
L Z
LZ
D000818:Animals; D001921:Brain / Q000201:enzymology*; D036145:Ginsenosides; D008297:Male; D008861:Microsomes / Q000201:enzymology; D005894:Panax; D010946:Plants, Medicinal; D051381:Rats; D011919:Rats, Inbred Strains; D012503:Saponins / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism*
D036145:Ginsenosides; D012503:Saponins; D000254:Sodium-Potassium-Exchanging ATPase; C035054:ginsenoside Rg1