Expression of the full-length products of the human papillomavirus type 6b (HPV-6b) and HPV-11 L2 open reading frames by recombinant baculovirus, and antigenic comparisons with HPV-11 whole virus particles.
The L2 open reading frames (ORFs) of human papillomavirus (HPV) types 6b and 11 were expressed as full-length non-fusion proteins in Spodoptera frugiperda (Sf-9) cells using recombinant baculovirus. Both proteins were detected on Western blots as immunoreactive bands which migrated with apparent Mrs of 76K and 78K, respectively, and contained both cross-reactive and type-specific epitopes, as determined by polyclonal antisera directed against defined subregions of the HPV-6b and HPV-11 L2 ORFs. In addition, the minor capsid protein of HPV-11 particles co-migrates with the HPV-11 L2 ORF product and is immunoreactive with HPV-11 L2-specific antisera. These observations indicate that the anomalous electrophoretic mobilities of papillomavirus L2 ORF proteins can be explained without invoking post-transcriptional processing events and that the minor capsid protein of HPV-11 is antigenically and biophysically related to the HPV-11 L2 ORF product.
Rose
R C
RC
Department of Medicine, University of Rochester School of Medicine, New York 14642.
Bonnez
W
W
Strike
D G
DG
Reichman
R C
RC
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics* / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics*; D002213:Capsid / Q000235:genetics* / Q000276:immunology; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003429:Cross Reactions; D006801:Humans; D009036:Moths; D016366:Open Reading Frames; D027383:Papillomaviridae / Q000235:genetics* / Q000276:immunology; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
D000956:Antigens, Viral; D011994:Recombinant Proteins