title: Detection of Epstein-Barr viral genomes in lymph nodes of Hodgkin's disease patients.
abstract: Previous studies using Southern blot analysis or in situ hybridization have shown that approximately 20% of patients with Hodgkin's disease have Epstein-Barr virus (EBV) in involved tissues. We used the more sensitive polymerase chain reaction (PCR) technique to determine if a higher percentage of EBV could be detected. Of the 16 Hodgkin's disease patients studied, the PCR technique detected EBV in eight (50%). No prognostic significance was associated with the presence of EBV in the eight EBV-positive patients, and the presence of EBV was not associated with B cell monoclonality.
authors.affiliation: Laboratoire d'Oncologie Moléculaire, Centre Jean Perrin, Clermont-Ferrand, France.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Bignon
authors.forename: D
authors.initials: D
authors.lastname: Bernard
authors.forename: H
authors.initials: H
authors.lastname: Curé
authors.forename: Y
authors.initials: Y
authors.lastname: Fonck
authors.forename: J
authors.initials: J
authors.lastname: Pauchard
authors.forename: P
authors.initials: P
authors.lastname: Travade
authors.forename: M
authors.initials: M
authors.lastname: Legros
authors.forename: B
authors.initials: B
authors.lastname: Dastugue
authors.forename: R
authors.initials: R
authors.lastname: Plagne
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001483:Base Sequence; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005814:Genes, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D006689:Hodgkin Disease / Q000235:genetics / Q000382:microbiology*; D006801:Humans; D008198:Lymph Nodes / Q000382:microbiology*; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral