title: Human parainfluenza virus induces a type-specific protective immune response.
abstract: Induction of type-specific and cross-protective immune responses against human parainfluenza viruses have been investigated. The envelope glycoproteins HN (76 kDa) and F0 (62 kDa) from parainfluenza type 2 virus were selectively solubilized with octylglucoside. Detergent-soluble envelope glycoproteins were used as vaccine antigens for intranasal immunization of hamsters. The immunized animals showed complete protection from challenge infection with prototype live virus but failed to demonstrate a significant level of protection against either human parainfluenza type 1 or type 3 virus. The sera and bronchial lavages of immunized animals also showed type-specific neutralizing antibodies. A similar type-specific protective immune response was also noted after primary infection with live virus. The results indicate that a multivalent parainfluenza virus vaccine is probably required for protection against natural infection.
authors.affiliation: Molecular Engineering Associates, Birmingham, Alabama 35205.
authors.forename: R
authors.initials: R
authors.lastname: Ray
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuoka
authors.forename: T L
authors.initials: TL
authors.lastname: Burnett
authors.forename: B J
authors.initials: BJ
authors.lastname: Glaze
authors.forename: R W
authors.initials: RW
authors.lastname: Compans
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D006224:Cricetinae; D003429:Cross Reactions; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D018108:Respirovirus / Q000254:growth & development / Q000276:immunology*; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000276:immunology; D010948:Viral Plaque Assay; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D006023:Glycoproteins; D014759:Viral Envelope Proteins; D014765:Viral Vaccines