Chromosomal localization of the human myeloperoxidase gene by in situ hybridization using oligonucleotide probes.
Oligonucleotide probes have been used to map the myeloperoxidase (MPO) gene locus to chromosome bands 17q21-22. This is in agreement with results reported using conventional cDNA probes. No evidence for the existence of a second MPO gene locus was obtained. Six synthetic 72-base oligonucleotides, corresponding to different exon regions of the MPO gene, were tritium-labeled and used as in situ hybridization probes. Synthetic oligonucleotide probes offer a useful alternative to conventional DNA probes for gene mapping.
Zaki
S R
SR
Department of Pathology, Emory University School of Medicine, Atlanta, Georgia.
Austin
G E
GE
Chan
W C
WC
Conaty
A L
AL
Trusler
S
S
Trappier
S
S
Lindsey
R B
RB
Swan
D C
DC
D002871:Chromosome Banding; D002886:Chromosomes, Human, Pair 17; D015342:DNA Probes; D006801:Humans; D009693:Nucleic Acid Hybridization; D009195:Peroxidase / Q000235:genetics*
D015342:DNA Probes; D009195:Peroxidase