title: Superoxide production by rat neutrophils in the oleic acid model of lung injury.
abstract: The purpose of this study was to investigate the superoxide anion (O2-)-generating capacity of neutrophils isolated from rats at various stages of oleic acid(OA)-induced lung injury. Neutrophils were collected from blood, bronchoalveolar lavage (BAL), and peritoneal cavity (glycogen induced) after OA administration. Control neutrophils were collected from the blood of normal animals as a representative of nonprimed cells that produce low levels of O2-. A second control was the glycogen-elicited peritoneal neutrophil of normal animals which represented primed cells that produce enhanced levels of O2-. The ability of the neutrophils to produce O2- was evaluated by using both myristate acetate and opsonized zymosan as stimulants. Neutrophils isolated from blood and BAL from OA-injured lungs produced low levels of O2- and resembled closely the circulating, nonprimed neutrophil. Myeloperoxidase levels were measured in plasma and BAL and were found to be elevated in BAL of OA-injured animals. The inability of neutrophils to produce high levels of O2- and the elevation of myeloperoxidase suggest that neutrophils present in the lung may have degranulated in response to prior activation and are therefore incapable of further superoxide production.
authors.affiliation: Department of Anesthesiology, University of Connecticut Health Center, Farmington 06032.
authors.forename: F L
authors.initials: FL
authors.lastname: Watts
authors.forename: B L
authors.initials: BL
authors.lastname: Oliver
authors.forename: G M
authors.initials: GM
authors.lastname: Johnson
authors.forename: R S
authors.initials: RS
authors.lastname: Thrall
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D005609:Free Radicals; D005856:Germ-Free Life; D006003:Glycogen / Q000494:pharmacology; D008168:Lung / Q000187:drug effects; D055370:Lung Injury; D008297:Male; D008954:Models, Biological; D009504:Neutrophils / Q000201:enzymology / Q000378:metabolism*; D019301:Oleic Acid; D009829:Oleic Acids / Q000633:toxicity*; D010100:Oxygen / Q000378:metabolism; D010529:Peritoneal Cavity; D009195:Peroxidase / Q000097:blood; D051381:Rats; D011916:Rats, Inbred F344; D013481:Superoxides / Q000378:metabolism*
chemical_list: D005609:Free Radicals; D009829:Oleic Acids; D013481:Superoxides; D019301:Oleic Acid; D006003:Glycogen; D009195:Peroxidase; D010100:Oxygen