Multiple mRNAs of rat brain alpha-crystallin B chain result from alternative transcriptional initiation.
Two major classes of mRNAs for the alpha-crystallin B chain (or alpha(B)crystallin), about 0.9 and 1.2 kilobases in length, are expressed in rat brain. To examine the structures of these mRNAs, we isolated cDNA clones from rat brain and genomic DNA from rat liver. Characterization of these clones as well as Northern blot analysis indicated that the various mRNAs differed in the lengths of their 5' leader sequences. RNase protection assays revealed that the gene for alpha-crystallin B chain contains multiple start sites. The transcriptional start sites of the longer mRNAs are preceded by a putative CAAT box and that of the shorter mRNA by a putative TATA box. The shorter mRNA encodes the alpha-crystallin B chain protein, whereas the longer mRNA contained three extra small open reading frames upstream of the AUG start codon for the protein. The shorter mRNA is abundant in lens, heart, muscle, and kidney, while the longer mRNAs are constitutively expressed at low levels in a wide variety of tissues. The shorter mRNA was increased by treatment with phorbol 12-myristate 13-acetate in rat C6 glioma cells. Since there is only a single copy of the alpha-crystallin B chain gene, our results indicate that the two classes of mRNAs are generated by alternative transcriptional initiation from different promoters and their expressions are regulated differentially.
Iwaki
A
A
Department of Pathology, Columbia University College of Physicians and Surgeons, New York, New York 10032.
Iwaki
T
T
Goldman
J E
JE
Liem
R K
RK
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D003001:Cloning, Molecular; D003459:Crystallins / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D015723:Gene Library; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D015183:Restriction Mapping; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014158:Transcription, Genetic
D003459:Crystallins; D005904:Glial Fibrillary Acidic Protein; D046911:Macromolecular Substances; D012333:RNA, Messenger; D003994:Bucladesine; D004247:DNA; D013755:Tetradecanoylphorbol Acetate