Identification of a receptor for protein import into mitochondria.
Anti-idiotypic antibodies, prepared using a chemically synthesized signal peptide of a mitochondrial precursor protein, recognized a mitochondrial integral membrane protein (p32). Fab fragments derived from both anti-idiotypic antibodies and monospecific antibodies against purified p32 inhibited protein import into mitochondria. Moreover, anti-p32 antibodies specifically immunoprecipitated a precursor-p32 complex after detergent solubilization of mitochondria. Immunoelectron microscopy and subfractionation of mitochondria indicate that p32 is located in contact sites between the outer and inner mitochondrial membranes.
Pain
D
D
Howard Hughes Medical Institute, Rockefeller University, New York, New York 10021.
Murakami
H
H
Blobel
G
G
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D001692:Biological Transport; D003576:Electron Transport Complex IV / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D007425:Intracellular Membranes / Q000378:metabolism; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism; D008854:Microscopy, Electron; D008928:Mitochondria / Q000378:metabolism*; D008970:Molecular Weight; D011498:Protein Precursors / Q000378:metabolism*; D021382:Protein Sorting Signals / Q000276:immunology; D011956:Receptors, Cell Surface / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D012441:Saccharomyces cerevisiae; D013367:Submitochondrial Particles / Q000378:metabolism
D000888:Antibodies, Anti-Idiotypic; D005656:Fungal Proteins; D046911:Macromolecular Substances; D008565:Membrane Proteins; D011498:Protein Precursors; D021382:Protein Sorting Signals; D011956:Receptors, Cell Surface; D003576:Electron Transport Complex IV