Stimulatory effect of serum albumin on the proliferation of serum-free SV40-transformed Balb/c 3T3 cells.
Commercial serum albumins have been found to be able to stimulate the proliferation of Balb/c 3T3 cells transformed by SV40, but not that of the normal counterpart. The effect is most pronounced with crystalline samples of albumin depleted of both globulin and fatty acid components, and depends on conditions used for the attachment and on seeding density. Physical and chemical treatments aimed to remove tightly bound impurities do not abolish the activity of fatty acid free serum albumin, thus supporting the idea that albumin per se is mitogenic towards these cells.
Bedotti
R
R
Department of Physics, University of Parma, Italy.
Borghetti
A F
AF
Favilla
R
R
D000818:Animals; D001769:Blood; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003460:Crystallization; D003470:Culture Media; D005227:Fatty Acids; D007328:Insulin / Q000494:pharmacology; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D012709:Serum Albumin / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D013539:Simian virus 40; D014168:Transferrin / Q000494:pharmacology
D003470:Culture Media; D005227:Fatty Acids; D007328:Insulin; D008041:Linoleic Acids; D012709:Serum Albumin; D014168:Transferrin; D012710:Serum Albumin, Bovine; D019787:Linoleic Acid