Monoclonal antibody to blood group glycosyltransferases, produced by hybrids constructed with Epstein-Barr-virus-transformed B lymphocytes from a patient with ABO-incompatible bone marrow transplant and mouse myeloma cells.
A patient with chronic myeloid leukemia secreted an antibody to blood group glycosyltransferases after ABO-incompatible bone marrow transplantation (B recipient/O donor). Peripheral B lymphocytes from the recipient were transformed with Epstein-Barr virus, and then fused by polyethylene glycol with mouse myeloma cell line P3-X63/Ag8.653. After the cloning of the hybridoma cells, a cell line which produced human IgM antibody to blood group glycosyltransferases was established. The antibody completely neutralized B transferase activity at low concentration, while a larger amount of immunoglobulins was required to neutralize A transferase activity.
Kominato
Y
Y
Department of Legal Medicine, Toyama Medical and Pharmaceutical University, Japan.
Fujikura
T
T
Shimada
I
I
Takizawa
H
H
Hayashi
K
K
Mori
T
T
Matsuda
T
T
D000017:ABO Blood-Group System / Q000276:immunology*; D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000276:immunology; D001787:Blood Group Incompatibility / Q000276:immunology; D016026:Bone Marrow Transplantation / Q000276:immunology; D002459:Cell Fusion; D002472:Cell Transformation, Viral; D005700:Galactosyltransferases / Q000276:immunology*; D004854:Herpesvirus 4, Human; D006801:Humans; D006825:Hybridomas / Q000378:metabolism*; D007136:Immunoglobulins / Q000032:analysis; D008297:Male; D051379:Mice; D017350:N-Acetylgalactosaminyltransferases; D010954:Plasmacytoma / Q000276:immunology
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D007136:Immunoglobulins; D005700:Galactosyltransferases; D017350:N-Acetylgalactosaminyltransferases; C003742:UDPgalactosamine-galactose acetylgalactosaminyltransferase; C023155:blood-group-substance alpha-D-galactosyltransferase