Sandwich enzyme-immunoassay of human urinary trypsin inhibitor (urinastatin) and urinastatin-like immunoreactive substance in mouse urine.
In the enzyme-immunoassay, using rabbit anti-urinastatin (anti-UT) Fab'-beta-D-galactosidase complex and rabbit anti-UT IgG-coupled polystyrene ball as a solid phase, the least measurable amount of UT was 0.3 ng per assay tube. The assay was used to detect UT-like immunoreactivity in the urine of mice. The urine contained a UT-like immunoreactive substance with trypsin inhibitory activity, the concentration of which was 2.3 +/- 0.3 micrograms/l (mean +/- SEM, n = 10). Excretion in the urine was affected by glucocorticoids. There was no immunoreactivity against rabbit anti-UT IgG in the urine of rats, guinea-pigs, rabbits, cats and dogs.
Shikimi
T
T
Department of Pharmacology, Shimane Medical University, Japan.
Suzuki
S
S
Takahashi
M
M
Kaneto
H
H
D000293:Adolescent; D000305:Adrenal Cortex Hormones / Q000494:pharmacology; D000315:Adrenalectomy; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000328:Adult; D000818:Animals; D002415:Cats; D002648:Child; D004285:Dogs; D004597:Electroshock; D005260:Female; D006023:Glycoproteins / Q000032:analysis* / Q000378:metabolism; D006168:Guinea Pigs; D006801:Humans; D007124:Immunoenzyme Techniques; D007140:Immunoglobulin Fab Fragments; D008297:Male; D051379:Mice; D008875:Middle Aged; D011817:Rabbits; D051381:Rats; D012016:Reference Values; D001616:beta-Galactosidase
D000305:Adrenal Cortex Hormones; D006023:Glycoproteins; D007140:Immunoglobulin Fab Fragments; D000324:Adrenocorticotropic Hormone; D001616:beta-Galactosidase; C028665:urinastatin