title: Antigenic competition between dengue and Coxsackie viruses for presentation to B cells by macrophages.
abstract: Macrophages (M phi) pulsed with dengue type 2 (DV) and Coxsackie B4 (CoxB) viruses present antigen to B lymphocytes leading to their clonal expansion as detected by counting antigen-specific IgM antibody plaque-forming cells (PFC). The present study was undertaken to investigate the site for competition in M phi between the two heterologous antigens, DV and CoxB, for their presentation to B cells. It was observed that DV-pulsed M phi presented antigen to B cells in mice depleted of T cells by treatment with anti-Thy 1.2 monoclonal antibodies. The B cells could not be stimulated in absence of M phi in mice treated with silica. The PFC counts for both the antigens were inhibited when M phi were pulsed simultaneously with DV and CoxB. PFC counts were increased by 53-120% by predigesting the antigens by trypsin. Inhibition of DV-specific response by CoxB was abrogated by predigesting CoxB. A marked reduction in DV-specific PFC response was observed when CoxB was superimposed on M phi pulsed with DV 24 h earlier. CoxB-specific PFC counts were not affected by superimposing DV on M phi pulsed with CoxB 24 h earlier. PFC response to the antigen given to M phi before glutaraldehyde fixation was not affected while that for the antigen given to glutaraldehyde-fixed M phi was markedly depressed. It is concluded that the competition between DV and CoxB for antigen presentation to B cells occurs in M phi at the level of antigen processing.
authors.affiliation: Postgraduate Department of Microbiology, K.G. Medical College, Lucknow, India.
authors.forename: N
authors.initials: N
authors.lastname: Rizvi
authors.forename: U C
authors.initials: UC
authors.lastname: Chaturvedi
authors.forename: A
authors.initials: A
authors.lastname: Mathur
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D001667:Binding, Competitive; D003716:Dengue Virus / Q000276:immunology*; D029822:Enterovirus B, Human / Q000276:immunology*; D005976:Glutaral / Q000494:pharmacology; D006462:Hemolytic Plaque Technique; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D014357:Trypsin
chemical_list: D000956:Antigens, Viral; D014357:Trypsin; D005976:Glutaral