Establishment and characterization of 12S adenoviral E1A immortalized rat submandibular gland epithelial cells.
Rat submandibular gland (RSMG) cells have been immortalized with a retrovirus vector which encodes the adenovirus 12S E1A gene product. The immortalized cells were epithelial-like in nature and displayed a beta-adrenergic coupled rise in intracellular cAMP upon exposure to norepinephrine. Western- and lectin-blotting experiments of cell lysates demonstrated the presence of blood group A-reactive oligosaccharides. Such oligosaccharides are characteristic of RSMG mucin-glycoproteins. These cells appear to be suitable for use in analysis of cell-specific factors which regulate RSMG glycosylation.
He
X J
XJ
Department of Dental Research, School of Medicine and Dentistry, University of Rochester, NY 14642.
Frank
D P
DP
Tabak
L A
LA
D000017:ABO Blood-Group System / Q000276:immunology; D017456:Adenovirus Early Proteins; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000378:metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D009638:Norepinephrine / Q000494:pharmacology; D009844:Oligosaccharides / Q000276:immunology / Q000378:metabolism; D009856:Oncogene Proteins, Viral / Q000494:pharmacology*; D051381:Rats; D013363:Submandibular Gland / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
D000017:ABO Blood-Group System; D017456:Adenovirus Early Proteins; D009844:Oligosaccharides; D009856:Oncogene Proteins, Viral; D000242:Cyclic AMP; D009638:Norepinephrine