Stimulation of topoisomerase II mediated DNA cleavage at specific sequence elements by the 2-nitroimidazole Ro 15-0216.
The effect of the 2-nitroimidazole Ro 15-0216 upon the interaction between purified topoisomerase II and its DNA substrate was investigated. The cleavage reaction in the presence of this DNA-nonintercalative drug took place with the hallmarks of a regular topoisomerase II mediated cleavage reaction, including covalent linkage of the enzyme to the cleaved DNA. In the presence of Ro 15-0216, topoisomerase II mediated cleavage was extensively stimulated at major cleavage sites of which only one existed in the 4363 base pair pBR322 molecule. The sites stimulated by Ro 15-0216 shared a pronounced sequence homology, indicating that a specific nucleotide sequence is crucial for the action of this drug. The effect of Ro 15-0216 thus differs from that of the clinically important topoisomerase II targeted agents such as mAMSA, VM26, and VP16, which enhance enzyme-mediated cleavage at a multiple number of sites. In contrast to the previous described drugs, Ro 15-0216 did not exert any inhibitory effect on the enzyme's catalytic activity. This observation might be ascribed to the low stability of the cleavage complexes formed in the presence of Ro 15-0216 as compared to the stability of the ones formed in the presence of traditional topoisomerase II targeted drugs.
Sørensen
B S
BS
Department of Molecular Biology and Plant Physiology, University of Aarhus, Denmark.
Jensen
P S
PS
Andersen
A H
AH
Christiansen
K
K
Alsner
J
J
Thomsen
B
B
Westergaard
O
O
D000083:Acetanilides / Q000494:pharmacology*; D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002417:Cattle; D004247:DNA; D004262:DNA Restriction Enzymes; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D004275:DNA, Ribosomal / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D009593:Nitroimidazoles / Q000494:pharmacology*; D010957:Plasmids; D012689:Sequence Homology, Nucleic Acid; D013379:Substrate Specificity; D013950:Thymus Gland / Q000201:enzymology; D014344:Trypanocidal Agents / Q000494:pharmacology
D000083:Acetanilides; D004275:DNA, Ribosomal; D009593:Nitroimidazoles; D014344:Trypanocidal Agents; C054646:Ro 15-0216; D004247:DNA; D004262:DNA Restriction Enzymes; D004250:DNA Topoisomerases, Type II