Differential utilization of calcitonin gene regulatory DNA sequences in cultured lines of medullary thyroid carcinoma and small-cell lung carcinoma.
Regulation of expression of the human calcitonin gene was found to differ between two tumor lines of different tissue origin, medullary thyroid carcinoma (TT line) and small-cell lung carcinoma (DMS53 line). Distal 5' DNA elements between -750 and -2000 exhibited a stronger basal activity in DMS53 than in TT cells, whereas proximal DNA sequences between -132 and -252 mediated a dramatic cyclic AMP response in TT but not DMS53 cells.
de Bustros
A
A
School of Medicine, Johns Hopkins University, Baltimore, Maryland 21231.
Lee
R Y
RY
Compton
D
D
Tsong
T Y
TY
Baylin
S B
SB
Nelkin
B D
BD
D002116:Calcitonin / Q000235:genetics*; D018288:Carcinoma, Small Cell / Q000235:genetics*; D002460:Cell Line; D004273:DNA, Neoplasm / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D005796:Genes; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D010957:Plasmids; D012045:Regulatory Sequences, Nucleic Acid; D013964:Thyroid Neoplasms / Q000235:genetics*; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured / Q000378:metabolism
D004273:DNA, Neoplasm; D002116:Calcitonin