Superoxide anion production by circulating polymorphonuclear leucocytes in rheumatoid arthritis.
In twelve rheumatoid arthritis (RA) patients, receiving only nonsteroid anti-inflammatory therapy, superoxide anion (02-) generation by polymorphonuclear leucocytes (PMNs) was assessed by Cytochrome C reduction. The 02- production by non-activated PMNs in RA patients was significantly higher than in healthy controls. The 02- production by PMNs activated by zymosan and phorbol myristate acetate was significantly lower than in controls. PMNs from controls preincubated with rheumatoid sera generated higher 02- levels than the same PMNs incubated in normal sera.
Cencetti
A
A
1st Medical Clinic, University of Florence, Italy.
De Martino
M
M
Graziani
E
E
Maddali Bongi
S
S
Palermo
C
C
Pavari
E
E
Zoppi
M
M
D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000097:blood* / Q000378:metabolism; D003574:Cytochrome c Group / Q000378:metabolism; D005260:Female; D006358:Hot Temperature; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism* / Q000502:physiology; D010003:Osteoarthritis / Q000097:blood / Q000378:metabolism; D010084:Oxidation-Reduction; D013482:Superoxide Dismutase / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D003574:Cytochrome c Group; D013481:Superoxides; D015054:Zymosan; D013482:Superoxide Dismutase; D013755:Tetradecanoylphorbol Acetate