Effect of linoleic acid hydroperoxide on adenovirus DNA replication.
The effect of linoleic acid hydroperoxide on replication of adenovirus type 2 DNA in HeLa cells was examined. The level of the DNA was extremely reduced when the cells immediately after the infection with adenovirus type 2 were incubated for 24 h in the presence of 1 nmol/ml (measured by the thiobarbituric acid reaction and expressed in terms of malondialdehyde) of the hydroperoxide. The levels of the messenger RNA for adenovirus type 2 DNA polymerase and the expressed polymerase were equally reduced. The inhibition of DNA replication by lipid hydroperoxides in animal cells was discussed in relation to degenerative disorders.
Sasaguri
Y
Y
Department of Pathology, Kurume University School of Medicine, Japan.
Yanagi
H
H
Fukuda
S
S
Nakano
R
R
Padmanabhan
R
R
Morimatsu
M
M
Yagi
K
K
D000260:Adenoviruses, Human / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D004257:DNA Polymerase II / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects*; D004279:DNA, Viral / Q000096:biosynthesis*; D005455:Fluorescent Antibody Technique; D005814:Genes, Viral; D006367:HeLa Cells; D006801:Humans; D008041:Linoleic Acids / Q000494:pharmacology*; D008054:Lipid Peroxides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D014158:Transcription, Genetic / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects
D004279:DNA, Viral; D008041:Linoleic Acids; D008054:Lipid Peroxides; D012333:RNA, Messenger; D012367:RNA, Viral; C008285:linoleic acid hydroperoxide; D004257:DNA Polymerase II