Methanol is a potent inhibitor of the metabolism of endogenous arachidonic acid by elicited rat peritoneal leukocytes.
The effect of methanol on the ability of elicited rat peritoneal leukocytes to metabolise endogenous and exogenous arachidonic acid was studied using 2H8-arachidonic acid as the source of exogenous arachidonic acid and calcium ionophore A23187 as the lipoxygenase stimulus. As the methanol concentration increased from 0 to 992 mM there was a slight decrease in the total amount of LTB4 and related compounds formed, however examination of the ratio of undeuterated to deuterated LTB4 formed revealed that as the methanol concentration increased from 0 to 992 mM, the percentage of undeuterated LTB4 present decreased significantly from 57 +/- 9% to 2 +/- 1%. Methanol interferes with the ability of these cells to utilise endogenous arachidonic acid even in the presence of the powerful stimulus calcium ionophore A23187 thus allowing the facile biosynthesis of a range of deuterium labelled arachidonic acid metabolites.
Dawson
M
M
Department of Rheumatology, Royal North Shore Hospital, St. Leonards, NSW, Australia.
McGee
C M
CM
Smith
M D
MD
Brooks
P M
PM
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism* / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D003864:Depression, Chemical; D004789:Enzyme Activation / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism*; D007975:Leukotriene B4 / Q000096:biosynthesis; D008084:Lipoxygenase / Q000378:metabolism; D000432:Methanol / Q000494:pharmacology*; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats
D001095:Arachidonic Acids; D007975:Leukotriene B4; D016718:Arachidonic Acid; D000001:Calcimycin; D008084:Lipoxygenase; D000432:Methanol