Heterogeneity of antigen molecules recognized by anti-tax1 monoclonal antibody Lt-4 in cell lines bearing human T cell leukemia virus type I and related retroviruses.
Using a monoclonal antibody, Lt-4, directed against human T cell leukemia virus type I (HTLV-I) trans-activator (tax1) antigen, we examined the expression of tax1 and related antigens in a variety of T cell lines bearing HTLV-I and related retroviruses, simian T cell leukemia virus type I (STLV-I) and HTLV-II, by immunofluorescence and immunoblot assays. Lt-4 reacted with all HTLV-I-bearing cell lines tested and five out of eight simian cell lines bearing STLV-I, but not with an HTLV-II-bearing cell line. Lt-4 detected 40 kd tax1 antigen molecules in most HTLV-I-bearing cell lines except one cell line that expressed 39 kd tax1 antigen. In the STLV-I-bearing T cell lines, tax1-related antigen molecules detected by Lt-4 were heterogeneous, having molecular weights in the range of 36-41 kd.
Tanaka
Y
Y
Department of Immunology, School of Hygienic Sciences, Kitasato University, Kanagawa.
Yoshida
A
A
Takayama
Y
Y
Tsujimoto
H
H
Tsujimoto
A
A
Hayami
M
M
Tozawa
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D015486:HTLV-I Antigens / Q000276:immunology*; D000882:Haplorhini; D015367:Human T-lymphotropic virus 2 / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D015458:Leukemia, T-Cell; D012190:Retroviridae / Q000276:immunology*; D015303:Simian T-lymphotropic virus 1 / Q000276:immunology; D013601:T-Lymphocytes / Q000382:microbiology*; D015534:Trans-Activators / Q000032:analysis / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015486:HTLV-I Antigens; D015534:Trans-Activators