Colorimetric micromethods for glutaraldehyde determination by means of phenol and sulfuric acid or phenol and perchloric acid.
Aqueous micromolar solutions of glutaraldehyde produce a yellow color when treated with 20% phenol in ethanol followed by concentrated sulfuric acid or with phenol in 70% perchloric acid. The absorbance at 482 or 479 nm, respectively, is linearly related to the glutaraldehyde concentration. Of the two methods developed, the sulfuric acid-phenol assay gives a higher sensitivity and the perchloric acid-phenol assay allows the determination of glutaraldehyde in the presence of sugars and proteins.
Boratyński
J
J
Department of Microbiology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw.
Zal
T
T
D000447:Aldehydes / Q000032:analysis*; D002241:Carbohydrates; D003124:Colorimetry / Q000379:methods*; D005976:Glutaral / Q000032:analysis*; D008832:Microchemistry; D010472:Perchlorates; D019800:Phenol; D010636:Phenols; D011506:Proteins; D013464:Sulfuric Acids
D000447:Aldehydes; D002241:Carbohydrates; D010472:Perchlorates; D010636:Phenols; D011506:Proteins; D013464:Sulfuric Acids; D019800:Phenol; C033158:sulfuric acid; D005976:Glutaral