Epstein-Barr virus (EBV) receptors on epithelial hybrid cells derived from nasopharyngeal carcinoma.
We previously established an Epstein-Barr virus (EBV) genome-positive epithelial hybrid cell line, designated NPC-KT, which was prepared by fusing primary epithelial cells derived from nasopharyngeal carcinoma (NPC) with an epithelial Ad-AH cell line. In this study, we measured the presence of the EBV receptors using radiolabelled EBV. It was determined that similar amounts of Burkitt's lymphoma (BL)-derived P3HR-1 or NPC-KT-derived EBV can attach BL-derived Raji cells, but that only P3HR-1 virus can attach to NPC-KT cells. In addition, the superinfection of NPC-KT cells with P3HR-1 virus could not be inhibited by pretreatment with a monoclonal antibody against C3d (OKB7). Raji cells adsorbed with OKB7 became much less susceptible to superinfection with P3HR-1 or NPC virus. The data suggest that EBV receptors unrelated to C3d receptor exist.
Takimoto
T
T
Department of Otolaryngology, School of Medicine, Kanazawa University, Japan.
Tanaka
S
S
Ishikawa
S
S
Umeda
R
R
D002460:Cell Line; D015933:Complement C3d; D004854:Herpesvirus 4, Human; D006801:Humans; D006822:Hybrid Cells / Q000648:ultrastructure*; D066298:In Vitro Techniques; D009303:Nasopharyngeal Neoplasms / Q000648:ultrastructure*; D011951:Receptors, Complement / Q000648:ultrastructure; D011991:Receptors, Virus / Q000302:isolation & purification*; D014162:Transfection
D011951:Receptors, Complement; D011991:Receptors, Virus; D015933:Complement C3d