Evaluation of enzyme-linked immunosorbent assays based on monoclonal antibodies for the serology and antigen detection in canine parvovirus infections.
An enzyme-linked immunosorbent assay (ELISA) system was developed for the detection of canine parvovirus (CPV) or CPV antigen in dog faeces and two other ELISA systems were developed for the detection of CPV-specific antibodies in dog sera. The ELISA's were based on the use of CPV-specific mouse monoclonal antibodies, which recognise different epitopes of the haemagglutinin of CPV and which also neutralise the virus. A double antibody sandwich (DAS) ELISA for the detection of CPV in dog faeces was compared with the haemagglutination (HA) test. The DAS-ELISA proved to be more specific, sensitive and easier to perform than the HA assay. An indirect ELISA and a competitive ELISA for the detection of CPV-specific antibodies in dog sera were compared with the haemagglutination inhibition (HI) test. Both ELISA systems proved to be specific and easy-to-use methods for the detection of CPV-specific antibodies. The indirect ELISA, specially, proved to be more sensitive than the HI test. The higher sensitivity and specificity of the ELISA's as compared to HA and HI tests, and their ease of use, make them suitable for routine use in the serology and diagnosis of CPV infections.
Rimmelzwaan
G F
GF
National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
Juntti
N
N
Klingeborn
B
B
Groen
J
J
UytdeHaag
F G
FG
Osterhaus
A D
AD
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D010321:Parvoviridae / Q000276:immunology*; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D013047:Specific Pathogen-Free Organisms
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral