Modulation of urokinase receptors on human synovial cells and osteoarthritic chondrocytes by diacetylrhein.
On the basis of both 125I-labelled urokinase-like plasminogen activator binding analysis and transmission electron microscopy of an urokinase-gold complex, we have shown the presence of specific receptors for human urokinase on the cell membrane of human synovial cells. By radio-ligand binding experiments we have shown the existence of similar receptors on the surface of human chondrocytes. In both cases the specific binding is attributable to interaction between the receptor and the A chain of the ligand, as previously shown in other cell model systems. Treatment of synoviocytes with 1,8-diacetoxy-anthraquinone-3-carboxylic acid (diacetylrhein) is able to reduce the number of surface urokinase receptors. At the same time the drug can reduce the fibrinolytic activity released into the culture medium of human synovial cells. Preliminary data indicate that chondrocytes from osteoarthritic patients have a larger number of urokinase receptors than chondrocytes of normal patients. Diacetylrhein can restore the receptor number to normal levels; the amount of urokinase in the synovial fluid of ostroarthritic patients is also reduced. We conclude that the use of this drug has the chance to significantly modify the natural history of osteoarthritis.
Del Rosso
M
M
Institute of General Pathology, University of Florence, Italy.
Fibbi
G
G
Magnelli
L
L
Pucci
M
M
Dini
G
G
Grappone
C
C
Caldini
R
R
Serni
U
U
Colombo
F
F
Borella
F
F
D000880:Anthraquinones / Q000494:pharmacology*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002356:Cartilage / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D005342:Fibrinolysis / Q000187:drug effects; D006046:Gold; D006801:Humans; D007457:Iodine Radioisotopes; D008854:Microscopy, Electron; D010003:Osteoarthritis / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D010960:Plasminogen Activators / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000187:drug effects*; D055293:Receptors, Urokinase Plasminogen Activator; D013583:Synovial Membrane / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D014568:Urokinase-Type Plasminogen Activator
D000880:Anthraquinones; D000894:Anti-Inflammatory Agents, Non-Steroidal; D007457:Iodine Radioisotopes; C524898:PLAUR protein, human; D011956:Receptors, Cell Surface; D055293:Receptors, Urokinase Plasminogen Activator; C025292:diacerein; D006046:Gold; D010960:Plasminogen Activators; D014568:Urokinase-Type Plasminogen Activator