Experimental infection in specific-pathogen-free chicks with avian reovirus and avian nephritis virus isolated from broiler chicks showing runting syndrome.
Avian reovirus (ARV) and avian nephritis virus (ANV) were individually isolated from runty 10-day-old broiler chicks. The ARV isolate, IR-R, the ANV isolate, IR-N, and the reference strain of ANV, G-4260, were inoculated orally into 1-day-old chicks of two specific-pathogen-free (SPF) chicken lines, 151 and PDL-1. Growth retardation without the presence of gross lesions was clearly observed at 7 and 14 days postinoculation (PI) in chicks of both lines inoculated with the IR-R strain. On the other hand, in chicks inoculated with IR-N strain, growth retardation was observed only in the chicks of line 151 at 7 and 14 days PI. Microscopically, nephritis was observed in both chicken lines at 7 and 14 days PI. When chicks that were inoculated with the IR-N strain at 1 day of age were inoculated with the IR-R strain at 3 days of age, growth retardation was observed in the chicks of line PDL-1 at 10 and 17 days PI. However, the growth retardation was less severe than in the group receiving a single inoculation of the IR-R strain.
Shirai
J
J
Poultry Disease Laboratory, National Institute of Animal Health, Gifu, Japan.
Obata
H
H
Nakamura
K
K
Furuta
K
K
Hihara
H
H
Kawamura
H
H
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D001835:Body Weight; D002645:Chickens; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary; D006130:Growth Disorders / Q000382:microbiology / Q000662:veterinary*; D007243:Infectious bursal disease virus / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity; D007668:Kidney / Q000382:microbiology; D008099:Liver / Q000382:microbiology; D008286:Malabsorption Syndromes / Q000382:microbiology / Q000662:veterinary; D011201:Poultry Diseases / Q000382:microbiology*; D012087:Reoviridae / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity*; D012088:Reoviridae Infections / Q000382:microbiology / Q000662:veterinary*; D013047:Specific Pathogen-Free Organisms
D000914:Antibodies, Viral