Efficient transformation of Agrobacterium tumefaciens by electroporation.
High-voltage electroporation was used to transform Agrobacterium tumefaciens strains A136 and A348, reaching the efficiency of 1-3 x 10(8) transformants/micrograms DNA. Transformation frequency was dependent on the electrical field strength and the pulse length. No significant reduction in transformation efficiency was observed when the transforming DNA contained sites sensitive to endonuclease AtuCI of A. tumefaciens.
Mersereau
M
M
Department of Biochemistry, University of Minnesota, St. Paul 55108.
Pazour
G J
GJ
Das
A
A
D004262:DNA Restriction Enzymes / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004560:Electricity; D005822:Genetic Vectors; D010957:Plasmids; D012231:Rhizobium / Q000235:genetics*; D014170:Transformation, Genetic
D004262:DNA Restriction Enzymes; C041666:endodeoxyribonuclease BclI; D015252:Deoxyribonucleases, Type II Site-Specific