title: One- and two-dimensional proton NMR studies of cys-102 S-methylated yeast isozyme-1 ferricytochrome c.
abstract: The effect of S-methylating cysteine-102 (cys-102) (SH----SSCH3) of yeast isozyme-1 (iso-1) ferricytochrome c has been studied using proton NMR spectroscopy. COSY, NOESY, and one-dimensional nuclear Overhauser effect (NOE) difference spectroscopies have all been used. The NMR spectrum of this derivative is very similar to that of native yeast iso-1 ferricytochrome c. The advantage of using the cys-102 S-methylated derivative is that it is unable to spontaneously dimerize in solution, like native iso-1 monomer does. This makes the derivative a simple, ideal protein for long NMR experiments. This work yields many proton resonance assignments for S-methylated yeast iso-1 monomer and confirms all of the assignments for iso-1 monomer that were previously made using only the one-dimensional NOE method.
authors.affiliation: Department of Chemistry, University of New Mexico, Albuquerque 87131.
authors.forename: S C
authors.initials: SC
authors.lastname: Busse
authors.forename: S J
authors.initials: SJ
authors.lastname: Moench
authors.forename: J D
authors.initials: JD
authors.lastname: Satterlee
mesh_terms: D003545:Cysteine; D003574:Cytochrome c Group / Q000302:isolation & purification / Q000378:metabolism*; D045304:Cytochromes c; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008745:Methylation; D011487:Protein Conformation; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins
chemical_list: C014059:CYC1 protein, S cerevisiae; D003574:Cytochrome c Group; D029701:Saccharomyces cerevisiae Proteins; D045304:Cytochromes c; D003545:Cysteine