Identification of the gene homologous to HSV major DNA binding protein in the BHV-1 genome.
By means of Southern blot hybridisation using a cloned herpes simplex virus (HSV) major DNA binding protein (MDBP) gene as probe, the putative MDBP gene of BHV-1 was located within the Hind III G fragment which mapped between 0.352 and 0.381 map units of the BHV-1 genome. Moreover, an antiserum raised to HSV MDBP precipitated a 120kD polypeptide in a radio-immunoprecipitation test.
Bandyopadhyay
S K
SK
Department of Clinical Veterinary Medicine, University of Cambridge, Great Britain.
Mittal
S K
SK
Field
H J
HJ
D000818:Animals; D001345:Autoradiography; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003429:Cross Reactions; D015342:DNA Probes; D004268:DNA-Binding Proteins / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D007242:Herpesvirus 1, Bovine / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D015531:Radioimmunoprecipitation Assay; D015183:Restriction Mapping; D018139:Simplexvirus / Q000235:genetics*
D015342:DNA Probes; D004268:DNA-Binding Proteins