Priming effect of 2,3-dibenzylbutane-1,4-diol (mammalian lignan) on superoxide production in human neutrophils.
We investigated the effect of 2,3-dibenzylbutane-1,4-diol (DBB), a mammalian lignan, on superoxide production and [Ca2+]i mobilization in human neutrophils. DBB did not generate superoxide production by itself, but enhanced the FMLP or A23187-induced superoxide production in a dose dependent manner. DBB did not influence the OAG-induced superoxide production. The priming effect of DBB was inhibited by W-7 or trifluoroperazine, but not by H-7 or staurosporine. And the priming effect of DBB was observed in the presence or absence of extracellular Ca2+. DBB enhanced the low dose FMLP-induced [Ca2+]i mobilization. These results suggest that the priming effect of DBB in human neutrophils may be caused by the activation of the calcium-calmodulin pathway but not the protein kinase pathway.
Morikawa
M
M
Department of Pharmacology, Tokyo College of Pharmacy, Japan.
Abe
M
M
Yamauchi
Y
Y
Inoue
M
M
Tsuboi
M
M
D000470:Alkaloids / Q000494:pharmacology; D001593:Benzyl Compounds / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002451:Cell Compartmentation / Q000187:drug effects; D004075:Diglycerides / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D017705:Lignans; D008031:Lignin / Q000494:pharmacology*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000378:metabolism*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D019311:Staurosporine; D013449:Sulfonamides / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism*; D014268:Trifluoperazine / Q000494:pharmacology
D000470:Alkaloids; D001593:Benzyl Compounds; D004075:Diglycerides; D017705:Lignans; D013449:Sulfonamides; C063012:2,3-dibenzylbutane-1,4-diol; D013481:Superoxides; D014268:Trifluoperazine; D000001:Calcimycin; D009240:N-Formylmethionine Leucyl-Phenylalanine; C017967:W 7; D008031:Lignin; D011493:Protein Kinase C; D019311:Staurosporine; D002118:Calcium