Secretory patterns of 1 alpha-hydroxycorticosterone in the isolated perifused interrenal gland of the dogfish, Scyliorhinus canicula.
An isolated in-vitro perifused interrenal gland preparation from the dogfish Scyliorhinus canicula was used to study production of quantitatively the major corticosteroid 1 alpha-hydroxycorticosterone (1 alpha-OH-B), measured by radioimmunoassay. Basal secretory rates were 877.1 +/- 145 (S.E.M.) fmol/mg per 15 min (n = 14) and the preparation remained viable for up to 22 h, as reflected in a brisk response to 10 microM cyclic AMP (cAMP) after this time. Steroid production responded in a dose-dependent manner to porcine ACTH, with 10 microM producing a maximum stimulation of 225% above the basal secretory rate. cAMP (10 microM) produced an increase of 278% above basal, while 1 microM forskolin increased basal secretory rates by 127%. [Val5]- and [Ile5]-angiotensin II (0.1 microM) increased 1 alpha-OH-B production by 120 and 372% respectively over basal secretory rates. Increasing the concentration of K+ in the perfusate from 8 mM to 12, 18, 28 and 40 mM produced a significant rise only at 28 mM. Alterations in the concentration of Na+ and osmolarity of the perifusion medium had inconsistent effects on steroid production. Increased concentrations of urea (from 360 to 720 mM) increased the basal secretory rate by 121%, whilst reducing the concentration of urea (from 360 to 90 mM) had no effect.
O'Toole
L B
LB
Department of Animal and Plant Sciences, University of Sheffield, Western Bank.
Armour
K J
KJ
Decourt
C
C
Hazon
N
N
Lahlou
B
B
Henderson
I W
IW
D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D005576:Colforsin / Q000494:pharmacology; D003345:Corticosterone / Q000096:biosynthesis / Q000378:metabolism*; D000242:Cyclic AMP / Q000494:pharmacology; D004284:Dogfish; D005260:Female; D066298:In Vitro Techniques; D007401:Interrenal Gland / Q000187:drug effects / Q000378:metabolism*; D009994:Osmolar Concentration; D010477:Perfusion; D011863:Radioimmunoassay; D012964:Sodium / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology
D000804:Angiotensin II; D005576:Colforsin; D014508:Urea; D000324:Adrenocorticotropic Hormone; D012964:Sodium; D000242:Cyclic AMP; D003345:Corticosterone