A nuclear protein derived from brain cells stimulates transcription of the human neurotropic virus promoter, JCVE, in vitro.
The 98-base pair enhancer/promoter sequence is critical for cell type-specific transcription of the human neurotropic viral promoter, JCVE, in glial cells. Transcriptionally active extracts were prepared from glial cells and used to identify cis- and trans-acting regulatory elements that are involved in the glial-specific activation of the JCVE promoter. Results indicate that multiple regulatory sequences within the 98-base pair repeat specifically bind to nuclear proteins present in glial cells and positively regulate viral early RNA synthesis. The central region of the repeat, designated domain-B, interacts with a 45-kDa nuclear protein present in brain cells. This brain-specific protein was purified by conventional and DNA affinity chromatography. Complementation of the highly purified protein with HeLa extract significantly increased JCVE promoter activity. Thus, association of the novel glial-origin transcription factor with its target sequence increases transcription of the JCVE promoter in a non-glial context.
Ahmed
S
S
Department of Biochemistry and Molecular Biology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
Rappaport
J
J
Tada
H
H
Kerr
D
D
Khalili
K
K
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D004742:Enhancer Elements, Genetic; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007577:JC Virus / Q000235:genetics*; D008969:Molecular Sequence Data; D009457:Neuroglia / Q000378:metabolism; D009687:Nuclear Proteins / Q000302:isolation & purification / Q000378:metabolism*; D015345:Oligonucleotide Probes / Q000138:chemical synthesis; D011120:Polyomavirus / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
D009687:Nuclear Proteins; D015345:Oligonucleotide Probes