Germ-line and somatic recombination induced by in vitro modified P elements in Drosophila melanogaster.
The P element insertion delta 2-3(99B) has previously been shown to activate incomplete P elements elsewhere in the genome. We show that this element, in conjunction with a second incomplete P element, P[CaSpeR], also induces recombination in the male germ line. The recombination is induced preferentially in the region of the P[CaSpeR] element. Recombinant chromosomes contain the P[CaSpeR] element in more than 50% of cases, and alternative models of transposon replication and preferential chromosome breakage are put forward to explain this finding. As is the case with male recombination induced by P-M dysgenic crosses, recombination appears to be premeiotic in a high proportion of cases. The delta 2-3(99B) element is known to act in somatic cells. Correspondingly, we show that the delta 2-3(99B)-P[CaSpeR] combination elevates the incidence of somatic recombination.
Sved
J A
JA
School of Biological Sciences, Sydney University, New South Wales, Australia.
Eggleston
W B
WB
Engels
W R
WR
D000818:Animals; D003433:Crosses, Genetic; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D005854:Germ Cells / Q000378:metabolism; D008297:Male; D009713:Nucleotidyltransferases / Q000378:metabolism; D011995:Recombination, Genetic; D019895:Transposases
D004251:DNA Transposable Elements; D009713:Nucleotidyltransferases; D019895:Transposases