Solubilization of arachidonate-CoA ligase from cell membranes, chromatographic separation from nonspecific long-chain fatty acid CoA ligase, and isolation of mutant cell line defective in arachidonate-CoA ligase.
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D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002458:Cell Fractionation / Q000379:methods; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology*; D002845:Chromatography; D002999:Clone Cells; D003066:Coenzyme A Ligases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D003902:Detergents; D017886:Durapatite; D005354:Fibrosarcoma; D006882:Hydroxyapatites; D051379:Mice; D009154:Mutation; D012995:Solubility; D014461:Ultracentrifugation / Q000379:methods
D003902:Detergents; D006882:Hydroxyapatites; D017886:Durapatite; D003066:Coenzyme A Ligases; C033896:arachidonate - CoA ligase