title: Single-step labeling of DNA using restriction endonucleases and T4 polynucleotide kinase.
abstract: DNA restriction fragments can be end-labeled in a combined digestion and labeling reaction with restriction endonuclease plus T4 polynucleotide kinase, omitting a dephosphorylation step. Both pBR322 and phage lambda DNA digests are efficiently labeled in one step, one tube and one incubation.
authors.affiliation: Dept. of Biochemistry, University of Kansas, Lawrence 66045-2106.
authors.forename: A
authors.initials: A
authors.lastname: Oommen
authors.forename: I
authors.initials: I
authors.lastname: Ferrandis
authors.forename: M J
authors.initials: MJ
authors.lastname: Wang
mesh_terms: D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D007553:Isotope Labeling / Q000379:methods*; D010770:Phosphotransferases; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D013604:T-Phages / Q000235:genetics
chemical_list: D004279:DNA, Viral; D010770:Phosphotransferases; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D004262:DNA Restriction Enzymes