Interaction of tRNA with domain II of 23S rRNA.
The interaction of tRNA with domain II of 23S rRNA in E. coli ribosomes has been probed using short, complementary DNA oligodeoxyribonucleotides. Specifically, cDNA oligomers to the region 801-811 of the 23S rRNA were used to ascertain the interaction of this region with tRNA. It was found that when tRNA was bound to the P site, considerable competition occurred between tRNA and the cDNA oligomers which base paired with the nucleotides 807-811. However, A-site bound tRNA neither displaced, nor was displaced, by cDNA oligomers to this region. Additionally, the binding of tRNA lacking the CACCA nucleotides on the 3' terminus was unaffected by the presence a cDNA oligomer complementary to nucleotides 803-811, indicating that the cDNA-tRNA competition was dependent on the 3' terminal nucleotides of tRNA.
Hill
W E
WE
Division of Biological Sciences, University of Montana, Missoula.
Tassanakajohn
A
A
Tapprich
W E
WE
D001483:Base Sequence; D004722:Endoribonucleases; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D015345:Oligonucleotide Probes; D012338:RNA, Ribosomal, 23S / Q000235:genetics / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D012360:RNA, Transfer, Phe / Q000378:metabolism*; D016914:Ribonuclease H; D012270:Ribosomes / Q000378:metabolism*
D015345:Oligonucleotide Probes; D012338:RNA, Ribosomal, 23S; D012360:RNA, Transfer, Phe; D012343:RNA, Transfer; D004722:Endoribonucleases; D016914:Ribonuclease H