Efficient incorporation of galactose into lipopolysaccharide by Escherichia coli K-12 strains with polar galE mutations.
Efficient incorporation of exogenous galactose into lipopolysaccharide was observed in a strain with a galE::Tn10 insertion and a strain with a deletion of galOPE. No incorporation was observed in a strain with a longer (galETK) deletion, indicating that incorporation into the galE mutants was due to a low level of expression of galTK from internal or fortuitous promoters. These galE mutants provided a convenient and specific way to radiolabel lipopolysaccharide.
Schnaitman
C A
CA
Department of Microbiology, University of Virginia Medical School, Charlottesville 22908.
Austin
E A
EA
D002462:Cell Membrane / Q000378:metabolism; D002872:Chromosome Deletion; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism*; D007700:Kinetics; D008070:Lipopolysaccharides / Q000096:biosynthesis*; D009154:Mutation; D009876:Operon
D004251:DNA Transposable Elements; D008070:Lipopolysaccharides; D005690:Galactose