title: c-erbB-2 oncoprotein expression in mammary and extramammary Paget's disease: an immunohistochemical study.
abstract: Over-expression of the c-erbB-2 oncogene occurs in a proportion of human adenocarcinomas and in breast carcinoma is associated with poorer prognosis. Sections of formalin-fixed, paraffin-embedded tumour tissue from 22 patients with mammary and extramammary Paget's disease have been stained immunohistochemically using a monoclonal antibody (NCL-CB11) raised against a synthetic peptide from the C-terminal end of the predicted sequence of the c-erbB-2 protein product. All 12 cases of mammary Paget's disease showed membrane staining of intra-epidermal cells, indicating c-erbB-2 over-expression. Sections of underlying ductal breast carcinoma were available in nine cases; all nine tumours were c-erbB-2 positive and in eight the in situ component was of comedo or solid type. There was membrane staining of tumour cells in four of the 10 cases of extramammary Paget's disease; staining intensity was generally weaker than that observed in the cases of mammary disease. The possible implications of these findings for the histogenesis of both mammary and extramammary Paget's disease are discussed.
authors.affiliation: Department of Pathology, University of Newcastle-upon-Tyne, UK.
authors.forename: L
authors.initials: L
authors.lastname: Keatings
authors.forename: J
authors.initials: J
authors.lastname: Sinclair
authors.forename: C
authors.initials: C
authors.lastname: Wright
authors.forename: I P
authors.initials: IP
authors.lastname: Corbett
authors.forename: C
authors.initials: C
authors.lastname: Watchorn
authors.forename: C
authors.initials: C
authors.lastname: Hennessy
authors.forename: B
authors.initials: B
authors.lastname: Angus
authors.forename: T
authors.initials: T
authors.lastname: Lennard
authors.forename: C H
authors.initials: CH
authors.lastname: Horne
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D066246:ErbB Receptors; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D010145:Paget Disease, Extramammary / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D010144:Paget's Disease, Mammary / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D011379:Prognosis; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism*
chemical_list: D011518:Proto-Oncogene Proteins; D066246:ErbB Receptors; D011505:Protein-Tyrosine Kinases