Binding properties of avian retroviral proteins. I. Preparation and basic characterization of ASLV NC(p12) and MA(p19).
Using SP-Sephadex column chromatography we isolated from an avian retrovirus, AMV(MAV), nucleic acid-binding proteins ASLV NC(p12) and MA(p19). As shown by several criteria, namely SDS-PAGE, PR(p15) protease activity, and nucleic acid binding assay with the use of both ss and ds DNAs, our NC(p12) and MA(p19) isolates are virtually pure proteins mutually not cross-contaminated. Rabbit anti-NC(p12) and anti-MA(p19) sera which we prepared did not cross-react mutually. We conclude that both NC(p12) and MA(p19) and antibodies against them are adequately pure preparations for investigating their nucleic acid binding specificities towards AMV(MAV) genomic RNA and MAV-1 proviral DNA using electron microscopy supported by computer analysis of electron micrographs.
Trávnícek
M
M
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
Korb
J
J
Felsberg
J
J
Ríman
J
J
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D001354:Avian Leukosis Virus / Q000235:genetics*; D009189:Avian Myeloblastosis Virus / Q000235:genetics* / Q000378:metabolism; D002213:Capsid / Q000302:isolation & purification / Q000378:metabolism*; D036022:Capsid Proteins; D002352:Carrier Proteins / Q000378:metabolism; D002645:Chickens; D002850:Chromatography, Gel; D004279:DNA, Viral / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D012367:RNA, Viral / Q000378:metabolism; D016601:RNA-Binding Proteins; D012191:Retroviridae Proteins / Q000302:isolation & purification / Q000378:metabolism*; D014758:Viral Core Proteins / Q000302:isolation & purification / Q000378:metabolism*; D014763:Viral Matrix Proteins / Q000302:isolation & purification / Q000378:metabolism*
D000914:Antibodies, Viral; D036022:Capsid Proteins; D002352:Carrier Proteins; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D012191:Retroviridae Proteins; D014758:Viral Core Proteins; D014763:Viral Matrix Proteins; C040520:avian retrovirus proteins; C054897:nucleocapsid protein, Avian retrovirus