title: [Physico-chemical characteristics of angiotensin-converting enzyme from the bovine lung].
abstract: The angiotensin-converting enzyme from bovine lung was isolated by chromatography with a 25-30% yield and purified 2200-2600-fold. The active molecule concentration in the enzyme preparations was 70-100% as could be judged from titration by inhibitor SQ 20,881. The molecular mass of the enzyme according to electrophoretic data is about 132 kDa; the maximal radius of the enzyme molecule as determined by electron microscopy is 68 +/- 9 A. Five enzyme isoforms with pI of 4.85, 4.7, 4.54, 4.38 and 4.3, respectively, were identified. The kinetic parameters of hydrolysis of three synthetic peptide substrates and the constants of activation of the substrate (Z-Phe-His-Leu) hydrolysis by chloride anions were determined.
authors.forename: O A
authors.initials: OA
authors.lastname: Kost
authors.forename: N A
authors.initials: NA
authors.lastname: Lamzina
authors.forename: T Z
authors.initials: TZ
authors.lastname: Sharafutdinov
authors.forename: V L
authors.initials: VL
authors.lastname: Tsuprun
authors.forename: N F
authors.initials: NF
authors.lastname: Kazanskaia
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors; D000818:Animals; D002417:Cattle; D006868:Hydrolysis; D007527:Isoenzymes / Q000737:chemistry / Q000302:isolation & purification; D007700:Kinetics; D008168:Lung / Q000201:enzymology*; D008970:Molecular Weight; D007703:Peptidyl-Dipeptidase A / Q000737:chemistry / Q000302:isolation & purification*; D013379:Substrate Specificity; D013722:Teprotide / Q000494:pharmacology
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D007527:Isoenzymes; D013722:Teprotide; D007703:Peptidyl-Dipeptidase A
vernacular_title: Fiziko-khimicheskie kharakteristiki angiotenzin-prevrashchaiushchego fermenta iz legkikh byka.