Calcium-ionophore A23187 induces dual changes in the release of 5-lipoxygenase and cyclooxygenase products by macrophages.
A23187-treated murine peritoneal macrophages release increased quantities of the immunoreactive 5-lipoxygenase metabolite leukotriene B4 (LTB4) and the immunoreactive cyclooxygenase products 6-keto prostaglandin F1 alpha (6-keto PGF1 alpha) and thromboxane B2 (TXB2) during a 40 min incubation period. The increase in release of LTB4 was marked already after 5 min of incubation and was maximal after 20 min. The increase in release of 6-keto PGF1 alpha and TXB2 started in most cases after 5 min of incubation and augmented gradually up to 40 min after incubation. The ratio of the increase of LTB4/6-keto PGF1 alpha and of LTB4/TXB2 revealed an increase in favor of LTB4 in the first 5 min of incubation.
van Hilten
J A
JA
Institute of Pharmacology, Erasmus University Rotterdam, Faculty of Medicine, The Netherlands.
Ben Efraim
S
S
Bonta
I L
IL
D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood; D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology*; D066298:In Vitro Techniques; D007975:Leukotriene B4 / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D013929:Thromboxane B2 / Q000378:metabolism
D007975:Leukotriene B4; D000001:Calcimycin; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D001094:Arachidonate 5-Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases