Localization of the NGFI-A protein in the rat brain.
Antibodies are used to localize the NGFI-A protein in the rat brain. The protein is found in a wide variety of neurons. However, not all neurons are stained. The protein is either absent or present at undetectable levels in glial cells. Neuronal nuclei stain intensely, cytoplasmic staining is lighter. Seizures cause a detectable increase in the intensity of staining.
Mack
K
K
Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, MO 63110.
Day
M
M
Milbrandt
J
J
Gottlieb
D I
DI
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001923:Brain Chemistry; D002467:Cell Nucleus / Q000032:analysis; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000276:immunology; D007150:Immunohistochemistry; D008297:Male; D008667:Metalloproteins / Q000032:analysis* / Q000276:immunology; D008969:Molecular Sequence Data; D009457:Neuroglia / Q000032:analysis; D009474:Neurons / Q000032:analysis; D010433:Pentylenetetrazole / Q000633:toxicity; D051381:Rats; D011919:Rats, Inbred Strains; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism; D013003:Somatosensory Cortex / Q000032:analysis
D000911:Antibodies, Monoclonal; D004268:DNA-Binding Proteins; D008667:Metalloproteins; D011993:Recombinant Fusion Proteins; D010433:Pentylenetetrazole