Type-C virus-like particles in a human B-cell lymphoma cell line.
Type-C virus-like particles (VLPs) were found in an Epstein-Barr (EB) virus-infected human B-cell lymphoma cell line, SP-50B, that was established from a patient with non-Hodgkin lymphoma. The cell line continuously produces a small number of type-C VLPs, 150-200 nm in diameter, over 1 year. SP-50B cells were negative for HTLV-I and HTLV-II antigens and did not contain the HTLV-I genome. In addition, two EB virus nuclear antigen (EBNA)-positive B-cell lines, SP-54-Cord and SP-57-CLL, were established from human cord blood and chronic lymphocytic leukemia (CLL), respectively, by coculture with lethally irradiated SP-50B cells. Type-C VLPs with the same morphology were also found in both cell lines.
Kubonishi
I
I
Department of Medicine, Kochi Medical School, Japan.
Ohtsuki
Y
Y
Sawada
T
T
Yano
S
S
Eguchi
T
T
Iwahara
Y
Y
Takeuchi
C
C
Miyoshi
I
I
D000974:Antibodies, Antinuclear / Q000032:analysis; D001402:B-Lymphocytes; D003584:Cytological Techniques; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D008223:Lymphoma / Q000276:immunology / Q000382:microbiology*; D008854:Microscopy, Electron; D010316:Particle Size; D014407:Tumor Cells, Cultured; D014771:Virion / Q000145:classification / Q000302:isolation & purification* / Q000648:ultrastructure
D000974:Antibodies, Antinuclear