title: Growth hormone and growth hormone binding protein in human urine.
abstract: The presence of growth hormone binding protein (GH-BP) in urine was demonstrated in normal subjects. Scatchard analysis showed that an association constant (Ka) and a maximum binding capacity (Bmax) of urinary GH-BP were 2.3 +/- 0.8 x 10(8) M-1 and 19.2 +/- 4.0 ng/mg urinary protein, respectively. The molecular weight of the urinary GH-BP was estimated to be 50000 to 70000 on gel chromatography. Although the molecular weight and Ka were similar to those of plasma GH-BP, Bmax of urinary GH-BP was significantly higher than that of plasma GH-BP (0.44 +/- 0.12 ng/mg protein). GH-BP in urine may be derived from plasma and/or renal tubules.
authors.affiliation: Department of Internal Medicine, Kyoto University Faculty of Medicine, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Hattori
authors.forename: A
authors.initials: A
authors.lastname: Shimatsu
authors.forename: Y
authors.initials: Y
authors.lastname: Kato
authors.forename: H
authors.initials: H
authors.lastname: Imura
mesh_terms: D000328:Adult; D002352:Carrier Proteins / Q000652:urine*; D002850:Chromatography, Gel; D013006:Growth Hormone / Q000652:urine*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008970:Molecular Weight
chemical_list: D002352:Carrier Proteins; D013006:Growth Hormone; C051815:somatotropin-binding protein