title: Cysteine mutants of herpes simplex virus type 1 glycoprotein D exhibit temperature-sensitive properties in structure and function.
abstract: We previously constructed seven mutations in the gene for glycoprotein D (gD) of herpes simplex virus type 1 in which the codon for one of the cysteine residues was replaced by a serine codon. Each of the mutant genes was cloned into a eucaryotic expression vector, and the proteins were transiently expressed in mammalian cells. We found that alteration of any of the first six cysteine residues had profound effects on protein conformation and oligosaccharide processing. In this report, we show that five of the mutant proteins exhibit temperature-sensitive differences in such properties as aggregation, antigenic conformation, oligosaccharide processing, and transport to the cell surface. Using a complementation assay, we have now assessed the ability of the mutant proteins to function in virus infection. This assay tests the ability of the mutant proteins expressed from transfected plasmids to rescue production of infectious virions of a gD-minus virus, F-gD beta, in Vero cells. Two mutant proteins, Cys-2 (Cys-106 to Ser) and Cys-4 (Cys-127 to Ser), were able to complement F-gD beta at 31.5 degrees C but not at 37 degrees C. The rescued viruses, designated F-gD beta(Cys-2) and F-gD beta(Cys-4), were neutralized as efficiently as wild-type virus by anti-gD monoclonal antibodies, indicating that gD was present in the virion envelope in a functional form. Both F-gD beta(Cys-2) and F-gD beta(Cys-4) functioned normally in a penetration assay. However, the infectivity of these viruses was markedly reduced compared with that of the wild type when they were preincubated at temperatures above 37 degrees C. The results suggest that mutations involving Cys-106 or Cys-127 in gD-1 confer a temperature-sensitive phenotype on herpes simplex virus. These and other properties of the cysteine-to-serine mutants allowed us to predict a disulfide bonding pattern for gD.
authors.affiliation: Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia 19104-6003.
authors.forename: D
authors.initials: D
authors.lastname: Long
authors.forename: G H
authors.initials: GH
authors.lastname: Cohen
authors.forename: M I
authors.initials: MI
authors.lastname: Muggeridge
authors.forename: R J
authors.initials: RJ
authors.lastname: Eisenberg
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001692:Biological Transport; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003545:Cysteine; D004252:DNA Mutational Analysis; D004220:Disulfides; D005455:Fluorescent Antibody Technique; D005816:Genetic Complementation Test; D009154:Mutation; D009500:Neutralization Tests; D011485:Protein Binding; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational; D018139:Simplexvirus / Q000235:genetics / Q000648:ultrastructure*; D013329:Structure-Activity Relationship; D013696:Temperature; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000648:ultrastructure
chemical_list: D000956:Antigens, Viral; D004220:Disulfides; D014759:Viral Envelope Proteins; C039447:glycoprotein D, Human herpesvirus 1; D003545:Cysteine