Studies on Saccharomyces cerevisiae under carbon-limiting growth transformed with plasmid pCYG4 that carries the gene for NADP-GDH.
The gene (GDH1) coding for the NADP-linked glutamate dehydrogenase system (NADP-GDH) has been cloned from Saccharomyces cerevisiae strain. Cells being transformed by the NADP-GDH gene on a 2 micron bared vector (pCYG4) plasmid confering 11-fold higher level on expressed GDH activity over the wild-type cells. The behavior of these cells was investigated under chemostatic growth with a carbon rate-limiting nutrient. Specific growth rates of cells carrying plasmid pCYG4 were found to be slightly slower than wild type cells. Furthermore, the NADP-GDH activity increases proportionally with the dilution rate. In addition, oscillations in the NADP-GDH activity, especially at a dilution rate up to 0.15/h, are probably consequential on the appearance of a changing mixed population (cells with and without plasmids).
Lima Filho
J L
JL
Departamento de Bioquimica, Universidade Federal de Pernambuco, Brazil.
Ledingham
W M
WM
D002244:Carbon; D004463:Ecology; D005800:Genes, Fungal / Q000502:physiology*; D005969:Glutamate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D018086:Glutamate Dehydrogenase (NADP+); D010957:Plasmids / Q000235:genetics*; D015202:Protein Engineering; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D014170:Transformation, Genetic
D002244:Carbon; D005969:Glutamate Dehydrogenase; D018086:Glutamate Dehydrogenase (NADP+)