Indirect inactivation of rat brain cytosolic diacylglycerol kinase by nucleoside-5'-monophosphate.
Cytosolic diacylglycerol kinase was inhibited drastically by nucleoside monophosphate. The inhibition was relatively specific for adenosine-5'-monophosphate (5'-AMP), although uridine-5'-monophosphate was also effective. The effect of 5'-AMP on diacylglycerol kinase appeared to be indirect since the degree of inhibition lessened with the dilution of the cytosol and the more purified enzyme failed to respond to 5'-AMP. A 5'-AMP-dependent mediator is proposed to be involved in the inactivation of diacylglycerol kinase.
Chen
Q
Q
Missouri Institute of Psychiatry, University of Missouri, Columbia.
Klemm
N
N
Jeng
I M
IM
D000249:Adenosine Monophosphate / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000201:enzymology*; D003600:Cytosol / Q000201:enzymology; D019852:Diacylglycerol Kinase; D010770:Phosphotransferases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051381:Rats
D000249:Adenosine Monophosphate; D010770:Phosphotransferases; D019852:Diacylglycerol Kinase