[Change in adenyl cyclase activity in the differentiation of neuroblastoma cells].
The change of Adenylcyclase from the plasma membrane of neuroblastoma cells during cell differentiation was investigated. As a parameter for the change of the Adenylcyclase activity we determined the Adenylcyclase stimulatability caused by the neurotransmitters noradrenaline, dopamine and adrenaline. In differentiated neuroblastoma cells the basilar Adenylcyclase activity was higher than in undifferentiated cells. The tested neurotransmitters showed the same result. By means of a G-protein from medullary adrenal gland we succeeded in reconstructing the Adenylcyclase from both undifferentiated and differentiated cells. The importance of the Gs protein for the cell differentiation is discussed.
Busse
E
E
Zentrum der Kinderheilkunde, J. W. Goethe-Universität, Frankfurt.
Kornhuber
B
B
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002395:Catecholamines / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D000242:Cyclic AMP / Q000502:physiology; D004789:Enzyme Activation / Q000502:physiology; D019204:GTP-Binding Proteins / Q000502:physiology; D006801:Humans; D051379:Mice; D009447:Neuroblastoma / Q000473:pathology*; D015290:Second Messenger Systems / Q000502:physiology; D014407:Tumor Cells, Cultured / Q000473:pathology*
D002395:Catecholamines; D000242:Cyclic AMP; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases
Anderung der Adenylcyclase-Aktivität während der Differenzierung von Neuroblastomzellen.