Detection of bovine viral diarrhea virus genome in leukocytes from persistently infected cattle by RNA-cDNA hybridization.
A bovine viral diarrhea virus (BVDV) cDNA library was constructed. One cloned complementary DNA sequence was used as a probe to detect BVDV RNA by hybridization in infected cell cultures and in mononuclear leukocytes from persistently infected cattle by dot blot and in situ hybridization. The cDNA probe hybridized with all cytopathic and noncytopathic BVDV isolates tested. The hybridization results were consistent with results obtained using conventional subculturing and immunofluorescent staining methods and by inoculation of seronegative test cattle.
Jensen
J
J
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison 53706.
Aiken
J
J
Schultz
R D
RD
D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000175:diagnosis* / Q000382:microbiology; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics* / Q000302:isolation & purification; D005455:Fluorescent Antibody Technique; D007962:Leukocytes / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010576:Pestivirus / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis*
D015342:DNA Probes; D004279:DNA, Viral; D012367:RNA, Viral