Protective role of ascorbic acid against asbestos induced toxicity in rat lung: in vitro study.
Asbestos fibers adsorb cytochrome P-450 and P-448 proteins from rat lung micosomal fractions and liberate heme from cytochrome P-448 on prolonged incubation in vitro. further, fibers, decrease the activities of benzo(a)pyrene hydroxylase and glutathione-S-transferase in microsomal and cytosolic fractions respectively. Mineral fibers also stimulate both the enzymatic (NADPH-induced) and non-enzymatic (Fe2(+)-induced) lipid peroxidation in microsomal fractions. Preincubation of microsomal and cytosolic fractions with a physiological concentration of ascorbic acid ameliorates, to a large extent, the changes induced by asbestos fibers.
Khan
S G
SG
Industrial Toxicology Research Centre, Lucknow, India.
Ali
S
S
Rahman
Q
Q
D000818:Animals; D001194:Asbestos / Q000633:toxicity*; D017638:Asbestos, Crocidolite; D017632:Asbestos, Serpentine; D001205:Ascorbic Acid / Q000494:pharmacology / Q000627:therapeutic use*; D019388:Cytochrome P-450 CYP1A2; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003580:Cytochromes / Q000378:metabolism; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects; D008168:Lung / Q000187:drug effects* / Q000201:enzymology; D008171:Lung Diseases / Q000209:etiology / Q000517:prevention & control; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D051381:Rats
D017632:Asbestos, Serpentine; D003580:Cytochromes; D017638:Asbestos, Crocidolite; D001194:Asbestos; D003577:Cytochrome P-450 Enzyme System; C017170:Cyp1a2 protein, rat; D019388:Cytochrome P-450 CYP1A2; D001205:Ascorbic Acid