title: Impaired polymorphonuclear leucocyte function in patients undergoing hepatectomy: adenylate energy charge and superoxide anion production in relation to hepatic mitochondrial redox state.
abstract: Patients undergoing hepatectomy have an increased susceptibility to infection. We therefore studied the energy metabolism of the polymorphonuclear leucocyte (PMN), focusing on energy charge and function, especially superoxide anion (O2-) generation, in relation to the hepatic mitochondrial redox state. By labelling the PMN adenine nucleotide pool with radioactive adenine and by superoxide dismutase-inhibitable reduction of ferricytochrome c, the energy charge and O2- production was measured in 18 patients with hepatoma (non-cirrhotic, seven; cirrhotic, 11) undergoing hepatectomy. Their arterial ketone body ratios (KBRs), reflecting the hepatic mitochondrial redox potential, were above 0.7 before operation. After surgery, the 18 patients were divided into two groups: group A, KBR greater than 0.7, n = 10; and group B, KBR less than 0.7, n = 8. The energy charge and O2- release in group B decreased significantly from preoperative values (P less than 0.001 and P less than 0.01 respectively) and when compared with group A (P less than 0.05 and P less than 0.01 respectively). These results suggest that impaired hepatic energy metabolism (KBR less than 0.7) in hepatectomized patients leads to impaired energy charge and O2- production in the PMNs.
authors.affiliation: Second Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Higashiyama
authors.forename: H
authors.initials: H
authors.lastname: Egawa
authors.forename: S
authors.initials: S
authors.lastname: Iwata
authors.forename: Y
authors.initials: Y
authors.lastname: Wada
authors.forename: Y
authors.initials: Y
authors.lastname: Shimahara
authors.forename: M
authors.initials: M
authors.lastname: Sasada
authors.forename: K
authors.initials: K
authors.lastname: Mori
authors.forename: K
authors.initials: K
authors.lastname: Ozawa
mesh_terms: D000227:Adenine Nucleotides / Q000097:blood*; D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000097:blood; D005260:Female; D006498:Hepatectomy; D006801:Humans; D007657:Ketone Bodies / Q000097:blood; D008104:Liver Cirrhosis, Alcoholic / Q000378:metabolism; D008113:Liver Neoplasms / Q000097:blood; D008297:Male; D008875:Middle Aged; D008930:Mitochondria, Liver / Q000378:metabolism*; D009504:Neutrophils / Q000502:physiology*; D010084:Oxidation-Reduction; D011184:Postoperative Period; D013481:Superoxides / Q000097:blood*
chemical_list: D000227:Adenine Nucleotides; D007657:Ketone Bodies; D013481:Superoxides