Cloning of Klebsiella pneumoniae pqq genes and PQQ biosynthesis in Escherichia coli.
We have cloned genes from Klebsiella pneumoniae which are required for pyrroloquinoline quinone (PQQ) biosynthesis. The cloned 6.7 kb fragment can complement several chromosomal pqq mutants. Escherichia coli strains are unable to synthesize PQQ but E. coli strains containing the cloned 6.7 kb K. pneumoniae fragment can synthesize PQQ in large amounts and E. coli pts mutants can be complemented on minimal glucose medium by this clone.
Meulenberg
J J
JJ
E.C. Slater Institute for Biochemical Research, University of Amsterdam, The Netherlands.
Sellink
E
E
Loenen
W A
WA
Riegman
N H
NH
van Kleef
M
M
Postma
P W
PW
D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D007709:Klebsiella / Q000235:genetics*; D045542:PQQ Cofactor; D015363:Quinolones; D015183:Restriction Mapping; D014169:Transformation, Bacterial
D015363:Quinolones; D045542:PQQ Cofactor; D004247:DNA