Effect of bovine herpesvirus-1 or parainfluenza-3 virus on immune receptor-mediated functions of bovine alveolar macrophages in the presence or absence of virus-specific serum or pulmonary lavage fluids collected after virus infection.
The immune receptor-mediated functions of bovine alveolar macrophages (AM) inoculated in vitro with bovine herpesvirus-1 (BHV-1) or parainfluenza-3 (PI-3) virus were tested in the presence or absence of virus-specific antiserum or pulmonary lavage fluids collected from calves 6 days after inoculation with BHV-1 or PI-3 virus. The Fc and C3b phagocytic indices of noninoculated AM, collected from 6- to 16-week-old calves, ranged from 75 to 87 and 59 to 64, respectively, and the binding indices ranged from 5 to 8 and 22 to 28, respectively. Infection of AM with either BHV-1 or PI-3 virus had no significant effect on receptor-mediated phagocytosis or binding, with the exception of a significant (P less than 0.05) decrease, from 64 to 46, of the C3b phagocytic index of PI-3 virus-infected AM. The addition of lavage fluids, collected after BHV-1 or PI-3 virus infection, to AM infected with the respective virus caused a significant (P less than 0.05) decrease in phagocytic indices with values for the Fc and C3b indices in BHV-1-infected AM decreasing from 81 to 49 and from 47 to 8, respectively, and those for the PI-3 virus-infected AM from 79 to 51 and from 46 to 15, respectively. The binding indices of virus-infected AM increased with the addition of viral lavage fluids, but the only significant (P less than 0.05) increase was for C3b binding in PI-3 virus-infected cells, which increased from 33 to 56.(ABSTRACT TRUNCATED AT 250 WORDS)
Brown
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TT
Department of Microbiology, Pathology and Parasitology, College of Veterinary Medicine, North Carolina State University, Raleigh 27606.
Shin
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D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D007242:Herpesvirus 1, Bovine / Q000302:isolation & purification / Q000502:physiology*; D007241:Infectious Bovine Rhinotracheitis / Q000276:immunology; D008264:Macrophages / Q000276:immunology / Q000502:physiology*; D010224:Parainfluenza Virus 3, Human / Q000302:isolation & purification / Q000502:physiology*; D018184:Paramyxoviridae Infections / Q000276:immunology / Q000662:veterinary; D010587:Phagocytosis / Q000502:physiology; D011971:Receptors, Immunologic / Q000276:immunology*; D013212:Staphylococcus epidermidis / Q000254:growth & development
D011971:Receptors, Immunologic