title: Involvement of activator protein in the activation of tryptophan hydroxylase by cAMP-dependent protein kinase.
abstract: The tryptophan hydroxylase activity of the crude extract from rat brain stem was stimulated approximately 2-fold by incubation with cAMP analogues under protein phosphorylating conditions. The cAMP-dependent activation process of the enzyme needed not only cAMP-dependent protein kinase but also activator protein. The kinetic properties of the enzyme activated by cAMP-dependent protein kinase were very similar to those of the enzyme activated by calmodulin-dependent protein kinase II.
authors.affiliation: Department of Biochemistry, Asahikawa Medical College, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Makita
authors.forename: S
authors.initials: S
authors.lastname: Okuno
authors.forename: H
authors.initials: H
authors.lastname: Fujisawa
mesh_terms: D000818:Animals; D001933:Brain Stem / Q000201:enzymology; D000242:Cyclic AMP / Q000378:metabolism; D002373:Cyclic AMP Receptor Protein / Q000378:metabolism*; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D014365:Tryptophan Hydroxylase / Q000378:metabolism*
chemical_list: D002373:Cyclic AMP Receptor Protein; D000242:Cyclic AMP; D014365:Tryptophan Hydroxylase; D011494:Protein Kinases