The effect of myc proteins on SV40 replication in human lymphoid cells.
We have previously demonstrated that over-expression of c-myc facilitates the replication of SV40 DNA in human Burkitt lymphoma cells (BL). In this paper we investigated the ability of N-myc and L-myc to substitute for c-myc in promoting SV40 DNA replication. Vectors expressing either a chimeric N-myc/c-myc, N-myc or L-myc gene were constructed and co-transfected with a plasmid containing the SV40 origin of replication and the early genes encoding SV40 T antigens (Tag). The chimeric N-myc/c-myc and the N-myc proteins enhance SV40 replication in the human lymphoma line BJAB at levels comparable to c-myc. The stimulative effect of N-myc is also evident in the BL cell line RAMOS. However, L-myc stimulated SV40 replication only in RAMOS cells.
Classon
M
M
Department of Tumor Biology, Karolinska Institutet, Stockholm, Sweden.
Henriksson
M
M
Klein
G
G
Sümegi
J
J
D004261:DNA Replication; D006801:Humans; D016393:Lymphoma, B-Cell / Q000235:genetics / Q000473:pathology; D009447:Neuroblastoma / Q000235:genetics / Q000473:pathology; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics / Q000502:physiology*; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000473:pathology; D014779:Virus Replication / Q000235:genetics*
D016271:Proto-Oncogene Proteins c-myc