title: Autoradiographic demonstration of specific binding sites for E. coli enterotoxin in various epithelia of the North American opossum.
abstract: In the North American opossum, heat-stable specific binding sites for E. coli enterotoxin are observed (i) in epithelial cells lining the small intestine, colon, gall bladder, cystic duct, common bile duct and trachea, and (ii) in epithelial cells forming the duodenal (Brunner's) glands, liver, kidneys (metanephros, mesonephros) and testis, as demonstrated by autoradiography. Enterotoxin-specific binding sites in the intestinal tract are only found in intestinal epithelial cells with the highest concentration in the microvillus border. Enterotoxin-specific binding sites also occur in epithelial cells comprising the secretory tubules and ducts of the duodenal glands. In the kidneys (metanephros and mesonephros), enterotoxin-specific binding sites are confirmed primarily to the proximal tubules, whereas in the testis they are localized in seminiferous tubules. In the liver, enterotoxin-specific binding sites are confined primarily to hepatocytes. E. coli enterotoxin caused a 7-fold increase of cGMP in the liver and a 30-fold increase in the duodenal glands. The liver responded in about half of the animals studied, whereas the duodenal glands gave a consistent response in each case. Likewise, the duodenal glands consistently showed strong labelling for 125I-enterotoxin, whereas receptor labelling of hepatocytes was inconsistent in nearly half the incubations and corresponds to the observed cGMP measurements.
authors.affiliation: Department of Anatomy, University of Missouri School of Medicine, Columbia 65212.
authors.forename: W J
authors.initials: WJ
authors.lastname: Krause
authors.forename: R H
authors.initials: RH
authors.lastname: Freeman
authors.forename: L R
authors.initials: LR
authors.lastname: Fort
mesh_terms: D000818:Animals; D001345:Autoradiography; D004768:Enterotoxins / Q000378:metabolism*; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism* / Q000648:ultrastructure; D004926:Escherichia coli; D006162:Guanylate Cyclase; D007457:Iodine Radioisotopes; D008297:Male; D009893:Opossums / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism; D000074261:Receptors, Enterotoxin; D050705:Receptors, Guanylate Cyclase-Coupled; D018000:Receptors, Peptide; D014018:Tissue Distribution
chemical_list: D004768:Enterotoxins; D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface; D018000:Receptors, Peptide; D006162:Guanylate Cyclase; D000074261:Receptors, Enterotoxin; D050705:Receptors, Guanylate Cyclase-Coupled