Electrically stimulated rapid gene expression in the brain: ornithine decarboxylase and c-fos.
A single electroconvulsive shock (ECS) resulted in a major induction of cerebral ornithine decarboxylase (ODC) mRNA and a rapid and transient elevation of ODC enzyme activity. The proto-oncogene c-fos was also transiently induced under the same conditions. Following a rapid rise in mRNA levels, the messages for these proteins take different courses. The c-fos mRNA fell to below control levels by 1 h, while the ODC mRNA remained elevated beyond 24 h. The ECS-induced elevation of ODC enzyme activity was not abolished by adrenalectomy but was attenuated significantly by the anti-convulsant MK-801. These results imply that the induction of cerebral ODC may be neuronal activity dependent, and suggest that the ODC/polyamine system may be linked to the proposed third messenger cascade, involving c-fos, which couples cell stimulation to gene expression, resulting in long-term adaptive responses.
Zawia
N H
NH
Department of Pharmacology, University of California, Irvine 92717.
Bondy
S C
SC
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology; D004247:DNA; D003986:Dibenzocycloheptenes / Q000494:pharmacology; D016291:Dizocilpine Maleate; D004558:Electric Stimulation; D005786:Gene Expression Regulation; D008297:Male; D009693:Nucleic Acid Hybridization; D009955:Ornithine Decarboxylase / Q000235:genetics* / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D051381:Rats
D003986:Dibenzocycloheptenes; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D016291:Dizocilpine Maleate; D004247:DNA; D009955:Ornithine Decarboxylase