Reversion of thermosensitive splicing defect of Moloney murine sarcoma virus ts110 by oversplicing of viral RNA.
Moloney murine sarcoma virus ts110 possesses a thermosensitive splicing defect. By continuously growing nonproducer cells at the nonpermissive temperature, a new class of revertant cells, termed 6m3, that had lost the thermosensitive splicing defect was produced, and six distinct clones were selected. These cell clones were transformed at either permissive or restrictive temperatures. Unlike parental 6m2 cells, which contain two virus-specific RNA species of 4.0 and 3.5 kilobases (kb) at temperatures permissive for transformation, the 3.5-kb RNA was the only virus-specific RNA species detected in 6m3 clones. No new v-mos-containing DNA fragment was observed in Southern blot analysis of these cell clones compared with parental 6m2 cells, indicating that the 3.5-kb RNA was a splicing product rather than a direct transcript. Moreover, these cells expressed P85gag-mos but not P58gag at any temperature. The reversion of the phenotype in 6m3 cell clones appears to be the result of a selective loss of the temperature sensitivity of the splicing reaction, without affecting the thermosensitivity of the protein kinase activity. This change also appears to alter the mechanism regulating the efficiency of the genomic RNA-splicing reaction.
Hamelin
R
R
Institut d'Oncologie Cellulaire et Moléculaire Humaine, Bobigny, France.
Honore
N
N
Sergiescu
D
D
Singh
B
B
Gerfaux
J
J
Arlinghaus
R B
RB
D000818:Animals; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D008980:Moloney murine sarcoma virus / Q000235:genetics* / Q000254:growth & development; D009154:Mutation; D012326:RNA Splicing; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D009053:Sarcoma Viruses, Murine / Q000235:genetics*; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013696:Temperature; D014764:Viral Proteins / Q000235:genetics / Q000302:isolation & purification
D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins; D015719:Single-Strand Specific DNA and RNA Endonucleases