Parainfluenza-3 infection of the guinea pig airways induces respiratory airway hyperreactivity in vitro.
Guinea pigs were inoculated intra-tracheally with bovine parainfluenza type 3 virus (PI-3) to investigate airway responsiveness to histamine and arecoline in vitro. Two days after saline or PI-3 inoculation no difference in the reactivity of the tracheal spirals was observed after cumulative concentration/response curves with both drugs. Similarly, contractions of parenchymal strips, induced by histamine, did not differ between both groups. However, 4 days after PI-3 inoculation the histamine induced contraction of the tracheal spirals was increased by 47% and the arecoline induced contractions by 32% as compared to the control group. Further, the contractions induced by histamine in parenchymal strips were significantly enhanced (p less than 0.01) in the PI-3 treated group. In conclusion, PI-3 infection of guinea pig respiratory tract induces hyperreactivity of the central and peripheral airways in vitro.
Folkerts
G
G
University of Utrecht, Faculty of Pharmacy, Dept. of Pharmacology, The Netherlands.
Nijkamp
F P
FP
D000818:Animals; D006168:Guinea Pigs; D006632:Histamine / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D010224:Parainfluenza Virus 3, Human; D018184:Paramyxoviridae Infections / Q000378:metabolism / Q000503:physiopathology*; D012137:Respiratory System / Q000378:metabolism / Q000503:physiopathology*; D014132:Trachea / Q000378:metabolism
D006632:Histamine