Reduced GABA-mediated chloride flux in entorhinal cortex-kindled rat brains.
Gamma-aminobutyric acid (GABA)-mediated chloride uptake was determined in synaptoneurosomes prepared from the brains of entorhinal cortex-kindled and paired control rats. Subjects were sacrificed either 24 h or 28 days after the experimental animals had exhibited their sixth stage 5 convulsion. In the cortex and cerebellum, no difference was observed between kindled and control animals at either time period. In the brain-stem, however, a significant reduction (approximately 50%) in chloride uptake was observed in kindled subjects both 24 h and 28 days after the last seizure. These findings are consistent with the hypothesis that the 'kindled state' results from a long-lasting decrease in GABA-mediated inhibitory activity.
Sneddon
W B
WB
Department of Pharmacology, University of Toronto, Ont., Canada.
Burnham
W M
WM
Prowse
S
S
Cheng
J
J
Kish
S H
SH
D000818:Animals; D001933:Brain Stem / Q000378:metabolism / Q000503:physiopathology*; D002540:Cerebral Cortex / Q000378:metabolism / Q000503:physiopathology*; D018118:Chloride Channels; D002712:Chlorides / Q000378:metabolism / Q000493:pharmacokinetics* / Q000502:physiology*; D007696:Kindling, Neurologic; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D013574:Synaptosomes / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
D018118:Chloride Channels; D002712:Chlorides; D008565:Membrane Proteins; D005680:gamma-Aminobutyric Acid