The influence of adrenalectomy on alpha-adrenoceptor responses in rat cerebral cortex slices.
Experiments were undertaken to examine the effect of depletion of adrenal steroids on neurotransmitter-stimulated cAMP accumulation in rat cerebral cortex slices. Long-term depletion due to bilateral adrenalectomy (ADX) and short term depletion due to the steroid synthesis inhibitor metopirone (MET) caused a significant increase in alpha 1-adrenoceptor-mediated potentiation of beta-adrenoceptor-stimulated cAMP accumulation. The effect of MET was prevented by corticosterone treatment. This effect of ADX was selective in that there was no change in alpha 1-adrenoceptor-mediated inositol phosphate accumulation. In addition, ADX had no effect on potentiation of isoprenaline-stimulated cAMP by either the GABAB agonist baclofen or the phorbol ester, 4 beta-phorbol dibutyrate. However, 2-chloroadenosine-stimulated cAMP accumulation was significantly reduced in ADX rats.
Robinson
J P
JP
Department of Physiology Medical School, Queen's Medical Centre, Nottingham, U.K.
Kendall
D A
DA
D000311:Adrenal Glands / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D003345:Corticosterone / Q000494:pharmacology / Q000502:physiology*; D000242:Cyclic AMP / Q000378:metabolism*; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D008797:Metyrapone / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011941:Receptors, Adrenergic / Q000187:drug effects / Q000502:physiology*; D013256:Steroids / Q000378:metabolism / Q000502:physiology*
D011941:Receptors, Adrenergic; D013256:Steroids; D000242:Cyclic AMP; D007545:Isoproterenol; D003345:Corticosterone; D009638:Norepinephrine; D008797:Metyrapone