title: Structural study of cell wall phosphomannan of Candida albicans NIH B-792 (serotype B) strain, with special reference to 1H and 13C NMR analyses of acid-labile oligomannosyl residues.
abstract: Chemical structures of manno-oligosaccharides, from biose to heptaose, released from the phosphomannan of Candida albicans NIH B-792 strain (serotype B) by mild acid hydrolysis were investigated. The results of 1H NMR, 13C NMR, and fast atom bombardment mass spectrometry analyses confirmed that these manno-oligosaccharides belong to a homologous beta-1,2-linked series. Although chemical shifts of 1H NMR patterns of these oligosaccharides were considerably too complicated to be assigned, their 13C NMR patterns were sufficiently simple to be interpreted, exhibiting a regular increase of downfield shift of ppm values of the C-1 atom from each mannopyranose residue in proportion to their molecular weights. In order to determine the whole chemical structure of the parent phosphomannan, the acid-stable domain was subjected to acetolysis and then enzymolysis with the Arthrobacter GJM-1 alpha-mannosidase and the resultant manno-oligosaccharides were investigated for their chemical structures by 1H NMR spectroscopy. The results of a precipitin-inhibition test using the beta-1,2-linked manno-oligosaccharides, from biose to hexaose, in comparison with the corresponding isomers containing alpha-1,2 linkage with small amounts of alpha-1,3 linkage, indicated that the haptens possessing the former linkage exhibited much higher inhibitory effects than the corresponding isomers containing the latter linkages did. Based on the present findings, a chemical structure of the phosphomannan of this C. albicans strain was proposed.
authors.affiliation: Second Department of Hygienic Chemistry, Tohoku College of Pharmacy, Miyagi, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
authors.forename: N
authors.initials: N
authors.lastname: Shibata
authors.forename: M
authors.initials: M
authors.lastname: Nakada
authors.forename: S
authors.initials: S
authors.lastname: Chaki
authors.forename: K
authors.initials: K
authors.lastname: Mizugami
authors.forename: Y
authors.initials: Y
authors.lastname: Ohkubo
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
mesh_terms: D001173:Arthrobacter / Q000201:enzymology; D002176:Candida albicans / Q000032:analysis*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D002473:Cell Wall / Q000032:analysis; D006859:Hydrogen; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008351:Mannans / Q000302:isolation & purification; D008361:Mannosidases; D013058:Mass Spectrometry; D008745:Methylation; D008969:Molecular Sequence Data; D043323:alpha-Mannosidase
chemical_list: D002247:Carbon Isotopes; D008351:Mannans; D006859:Hydrogen; C005437:phosphomannan; D008361:Mannosidases; D043323:alpha-Mannosidase