[Neutralization of toxic action of endotoxins of gram-negative bacteria by unithiol and magnesium sulphate].
The experiments were carried out on mice of CBA strain. The animals received lysate of S. typhimurium in the dose of LD50. The injection of lysate was followed by an increase in the level of malonic dialdehyde (MDA) suggesting the lipid peroxidation (LPO) activation in the liver. At the same time the concentration of cyclic adenosine monophosphate (cAMP) in the liver and the lungs was decreasing, which was indicative of the decrease in the activity of prostaglandins. The injections of unithiol prevented both the activation of LPO and the decrease in the concentration of cAMP, that effect having been caused by the antioxidant action of unithiol. The repeated injection of unithiol protected the animals from death in intoxication by lysate of S. typhimurium and Shigella sonnei; the protection manifested itself in the 4.2 and 3.7-fold increase in LD50 respectively. Magnesium sulphate enhanced the protective action of unithiol.
Grutman
M I
MI
Persidskiĭ
Iu V
IuV
Frolov
V M
VM
Nagornaia-Persidskaia
R N
RN
Gushla
E P
EP
D000818:Animals; D000242:Cyclic AMP / Q000032:analysis; D004731:Endotoxins / Q000037:antagonists & inhibitors*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D008278:Magnesium Sulfate / Q000494:pharmacology*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D051379:Mice; D012486:Salmonella typhimurium; D012764:Shigella sonnei; D014494:Unithiol / Q000494:pharmacology*
D004731:Endotoxins; D014494:Unithiol; D008315:Malondialdehyde; D008278:Magnesium Sulfate; D000242:Cyclic AMP
Neĭtralizatsiia toksicheskogo deĭstviia éndotoksinov gramotritsiatel'nykh bakteriĭ s pomoschch'iu unitiola i sul'fata magniia.