[Analysis for the integrated hepatitis B virus genome in cells of established human hepatocellular carcinoma cell line JHH-7].
Authors have successfully established 7 human hepatocellular carcinoma (HCC) cell lines. In one of these cell lines, JHH-7, derived from HBs antigen positive HCC, hepatitis B virus (HBV) genome was analyzed by Southern blot hybridization. Digesting with Hind III restriction endonuclease, two bands were obtained at 6.0 and 2.5 kb, that is the integration of HBV genome was confirmed at the two sites of chromosomes in this cell line. And analyzing with 32P labeled HBV-DNA fragments for probes, it was indicated that integrated HBV genome was incomplete one containing from X to C region.
Fujise
K
K
First Department of Internal Medicine, Jikei University School of Medicine.
Nagamori
S
S
Hasumura
S
S
Homma
S
S
Fujita
K
K
Sujino
H
H
Matsuura
T
T
Shimizu
K
K
Niiya
M
M
Ohno
T
T
D015139:Blotting, Southern; D006528:Carcinoma, Hepatocellular / Q000382:microbiology*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis*; D005814:Genes, Viral; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000382:microbiology*; D014407:Tumor Cells, Cultured
D004279:DNA, Viral; D004262:DNA Restriction Enzymes