Tissue-specific expression in transgenic mice directed by the 5'-flanking sequences of the human gene encoding interphotoreceptor retinoid-binding protein.
Interphotoreceptor retinoid-binding protein (IRBP) is an extracellular protein that has been suggested to participate in the visual process as a carrier for visual retinoids. A chimeric gene composed of the human IRBP promoter fused to the bacterial reporter gene chloramphenicol acetyltransferase (CAT) was used to generate transgenic mice. Analysis of six transgenic families revealed that the CAT gene, concomitant with the endogenous IRBP gene, was expressed primarily in the retina and, to a lesser extent, in the pineal gland. These results establish that a 1.3-kilobase fragment from the 5' end of the human IRBP gene is sufficient to direct transgene expression to a visual subdivision of the central nervous system.
Liou
G I
GI
Cullen Eye Institute, Baylor College of Medicine, Houston, Texas 77030.
Geng
L
L
al-Ubaidi
M R
MR
Matragoon
S
S
Hanten
G
G
Baehr
W
W
Overbeek
P A
PA
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002678:Chimera; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D003433:Crosses, Genetic; D015870:Gene Expression; D005796:Genes; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity; D010786:Photoreceptor Cells / Q000378:metabolism*; D010957:Plasmids; D018168:Receptors, Retinoic Acid; D015183:Restriction Mapping; D014212:Tretinoin / Q000378:metabolism
D002352:Carrier Proteins; D018168:Receptors, Retinoic Acid; D014212:Tretinoin; D015500:Chloramphenicol O-Acetyltransferase