Isolation of a Rhodobacter capsulatus bioB mutant and cloning of the bioB gene.
We isolated a Tn5 insertion mutant of Rhodobacter capsulatus which requires biotin for growth. Crossfeeding studies with Escherichia coli bio mutants indicated that the insertion is in the bioB gene. A cosmid that complements the bioB insertion was isolated, and the bioB gene was localized to a 2.85-kilobase EcoRI-PstI fragment.
Cardin
R D
RD
Department of Microbiology, Louisiana State University, Baton Rouge 70803.
Biel
A J
AJ
D001429:Bacteriochlorophylls / Q000096:biosynthesis; D003001:Cloning, Molecular; D003360:Cosmids; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D007700:Kinetics; D009154:Mutation; D012241:Rhodopseudomonas / Q000235:genetics* / Q000302:isolation & purification
D001429:Bacteriochlorophylls; D004251:DNA Transposable Elements