Inhibition of the human sperm acrosome reaction by a high molecular weight factor from human seminal plasma.
Human seminal plasma possesses a factor (acrosome reaction-inhibitory factor) that is precipitated by high speed centrifugation and that inhibits the ionophore- and dbc AMP-induced acrosome reaction of capacitated human spermatozoa but only if it is added toward the end of the capacitation period. Acrosome reaction-inhibitory factor can be partially purified by cation exchange chromatography and appears to differ from another factor that can be obtained by ultracentrifugation of human seminal plasma and that prevents the fertilization of mouse gametes.
Han
H L
HL
Department of Obstetrics and Gynecology, Rush-Presbyterian St. Luke's Medical Center, Chicago, Illinois 60612.
Mack
S R
SR
De Jonge
C
C
Zaneveld
L J
LJ
D000177:Acrosome / Q000187:drug effects / Q000502:physiology*; D003994:Bucladesine / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002852:Chromatography, Ion Exchange; D006801:Humans; D008297:Male; D008970:Molecular Weight; D010949:Plasma / Q000378:metabolism; D012661:Semen / Q000378:metabolism*; D013075:Sperm Capacitation; D013094:Spermatozoa / Q000502:physiology*; D014461:Ultracentrifugation
D000001:Calcimycin; D003994:Bucladesine