Evaluation of determination of lactate dehydrogenase isoenzyme 1 by chemical inhibition with perchlorate or with 1,6-hexanediol.
We have evaluated the determination of lactate dehydrogenase (EC 1.1.1.27) isoenzyme 1 activity by chemical inhibition of the other isoenzymes with perchlorate and with 1,6-hexanediol. In the hexanediol method, we studied the effect of the duration of incubation with the inhibitor; a 5-min incubation yielded results closest to those of an immunochemical technique (Isomune-LD). The perchlorate method was the most precise, and the hexanediol method the least, although for none of the techniques did the coefficient of variation exceed the medically acceptable limit prescribed by the College of American Pathologists. Pairwise correlation among the immunoprecipitation, electrophoretic, and chemical inhibition methods was good (r greater than 0.991), although the differences between means were statistically significant (except for the comparison of the two chemical inhibition methods). Because of its ease, low cost, and precision, we recommend the perchlorate method for routine use.
Paz
J M
JM
Service of Laboratory Central, Hospital General de Galicia, Santiago de Compostela, Spain.
Garcia
A
A
Gonzales
M
M
Treviño
M
M
Tutor
J C
JC
Jaquet
M
M
Rodriguez-Segade
S
S
D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D005188:False Negative Reactions; D005260:Female; D006018:Glycols; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000032:analysis* / Q000037:antagonists & inhibitors; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000201:enzymology; D010472:Perchlorates; D011773:Pyruvates; D012016:Reference Values; D017670:Sodium Compounds
D006018:Glycols; D007527:Isoenzymes; D010472:Perchlorates; D011773:Pyruvates; D017670:Sodium Compounds; C031068:sodium perchlorate; D007770:L-Lactate Dehydrogenase; C027765:hexamethylene glycol