title: [Physical mapping of strain AD169 human cytomegalovirus DNA].
abstract: The whole human cytomegalovirus strain AD169 genome was cloned into plasmid pAT153 in the form of 25 HindIII fragments. Double and triple digestions of the recombinant plasmids with restriction endonucleases BamHI, BglII, ClaI, DraI, EcoRI, EcoRV, HindIII, HpaI, KpnI, PaeR7, PstI, SphI and XbaI yielded a detailed restriction map of human cytomegalovirus DNA. Knowing the exact position of numerous restriction sites in the viral DNA molecule, we have been able to examine very closely the heterologous region between the long and the short segments of the human cytomegalovirus genome.
authors.affiliation: Centre de recherche en virologie, Institut Armand-Frappier, Laval-des-Rapides (Québec), Canada.
authors.forename: M
authors.initials: M
authors.lastname: Dion
authors.forename: C
authors.initials: C
authors.lastname: Hamelin
mesh_terms: D003001:Cloning, Molecular; D003587:Cytomegalovirus / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D006801:Humans; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
chemical_list: D004279:DNA, Viral
vernacular_title: Cartographie physique de l'ADN du cytomégalovirus humain souche AD169.