Acetyl-L-carnitine increases cytochrome oxidase subunit I mRNA content in hypothyroid rat liver.
The effect of acetyl-L-carnitine on the quantity of the messenger RNA for the subunit I of cytochrome oxidase in the liver mitochondria of hypothyroid rat was measured by Northern blot and solution hybridization. Three hours after pre-treatment of hypothyroid rat with acetyl-L-carnitine, the level of the transcript increased strongly. This effect was also obtained when acetyl-L-carnitine was administered to T3 pre-treated hypothyroid rats. These results add further evidence to the suggestion that acetyl-L-carnitine is able to stimulate mitochondrial transcription under altered metabolic conditions.
Gadaleta
M N
MN
Dipartimento di Biochimica e Biologia Molecolare, Università di Bari, Italy.
Petruzzella
V
V
Fracasso
F
F
Fernandez-Silva
P
P
Cantatore
P
P
D000108:Acetylcarnitine / Q000494:pharmacology*; D000818:Animals; D004272:DNA, Mitochondrial / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000235:genetics*; D015870:Gene Expression; D007037:Hypothyroidism / Q000201:enzymology / Q000235:genetics*; D008297:Male; D008930:Mitochondria, Liver / Q000502:physiology*; D009693:Nucleic Acid Hybridization; D051381:Rats; D011919:Rats, Inbred Strains; D014284:Triiodothyronine / Q000494:pharmacology
D004272:DNA, Mitochondrial; D014284:Triiodothyronine; D000108:Acetylcarnitine; D003576:Electron Transport Complex IV