Isolation and RNA sequence analysis of cytochrome b mutants resistant to funiculosin, a center i inhibitor of the mitochondrial ubiquinol-cytochrome c reductase in Saccharomyces cerevisiae.
Funiculosin is a well-known inhibitor of the mitochondrial respiratory chain, probably acting at the ubiquinone reducing site or center i of QH2-cytochrome c reductase. We report here the isolation, mapping and RNA sequence analysis of yeast apo-cytochrome b mutants resistant to this inhibitor. Funiculosin-resistance was found to be conferred, in 4 independent isolates, upon replacement of a leucine residue by phenylalanine in position 198 of the cytochrome b polypeptide chain.
di Rago
J P
JP
University of Louvain, Laboratoire de Génétique Microbienne, Louvain-la-Neuve, Belgium.
Perea
J
J
Colson
A M
AM
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D000880:Anthraquinones / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D003573:Cytochrome b Group / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics; D014450:Electron Transport Complex III / Q000037:antagonists & inhibitors*; D007425:Intracellular Membranes / Q000378:metabolism / Q000648:ultrastructure; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D009154:Mutation; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D011487:Protein Conformation; D012331:RNA, Fungal / Q000235:genetics* / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid
D000880:Anthraquinones; D000935:Antifungal Agents; D003573:Cytochrome b Group; D012331:RNA, Fungal; D009243:NAD; C015286:funiculosin (anthraquinone); D014450:Electron Transport Complex III