Sodium/potassium adenosine triphosphatase alpha-subunit and alpha-subunit mRNA levels in early rabbit embryos.
Sodium/potassium adenosine triphosphatase (Na+/K+ ATPase) and Na+/K+ ATPase mRNA content of rabbit embryos during preimplantation development were evaluated. Changes in Na+/K+ ATPase alpha-subunit content were detected with Western blotting using polyclonal antiserum against guinea pig Na+/K+ ATPase. Total RNA samples from rabbit embryos were analyzed by using Northern blots hybridized with random primer-labeled cDNA for Na+/K+ ATPase alpha-subunit from sheep kidney. Northern blots exhibited a single mRNA band (3.65 kilobases) in sheep kidneys and rabbit embryos. Between Day 4 and Day 6 of development, Na+/K+ ATPase alpha-subunit mRNA content increased 35-fold whereas Na+/K+ ATPase alpha-subunit content increased 22-fold. The similar increase in Na+/K+ ATPase alpha-subunit mRNA and alpha-subunit content in rabbit embryos suggests that Na+/K+ ATPase is partly regulated at the mRNA level during blastocyst expansion.
Gardiner
C S
CS
Department of Animal Science, Oregon State University, Corvallis 97331-6702.
Grobner
M A
MA
Menino
A R
AR
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D004622:Embryo, Mammalian / Q000032:analysis / Q000378:metabolism*; D047108:Embryonic Development / Q000502:physiology; D005260:Female; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D011817:Rabbits; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D000254:Sodium-Potassium-Exchanging ATPase