Characterization of the sequence of colonization and nosocomial candidemia using DNA fingerprinting and a DNA probe.
The objective of this hospital-based study was to determine the relationship between colonizing and infecting strains of Candida species and Torulopsis glabrata. Surveillance cultures from high-risk patients were paired with subsequent bloodstream isolates. Organisms were typed by using restriction endonuclease digestion of chromosomal DNA with BstNI and EcoRI, followed by Southern hybridization with a DNA probe (pBD4) derived from Saccharomyces cerevisiae. Sixteen patients for whom documented colonization preceded documented bloodstream infection were identified. The mean time between obtainment of surveillance isolates and obtainment of bloodstream isolates was 8 days, with a range of 1 to 423 days. For 15 (94%) of 16 patients, the DNA fingerprint pattern (using BstNI) of the surveillance isolate was identical to that of the bloodstream isolate. Isolates from 13 (81%) of 16 patients were unique to those patients. Typing by Southern hybridization with the pBD4 probe was less discriminating. We conclude that for a well-defined subset of hospitalized patients who were colonized by Candida species before developing nosocomial candidemia, the colonizing and infecting strains were identical, suggesting endogenous acquisition of infection. Restriction endonuclease digestion of chromosomal DNA was shown to be a discriminating and reproducible typing method for Candida species and T. glabrata.
Reagan
D R
DR
Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242.
Pfaller
M A
MA
Hollis
R J
RJ
Wenzel
R P
RP
D015139:Blotting, Southern; D002175:Candida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002177:Candidiasis / Q000382:microbiology*; D003428:Cross Infection / Q000382:microbiology*; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D004271:DNA, Fungal / Q000235:genetics; D006801:Humans
D015342:DNA Probes; D004271:DNA, Fungal; D004262:DNA Restriction Enzymes