Modulation of the beta-adrenergic stimulation of cyclic AMP accumulation in rat prostatic epithelial cells by membrane fluidity.
1. The influence of membrane lipid composition on the beta-adrenergic stimulation of cyclic AMP accumulation in rat prostatic epithelial cells was assessed after treating the cells with cholesteryl hemisuccinate (ChH). 2. ChH treatment resulted in a 40% inhibition of the beta-adrenergic response after 30 min of lipid preincubation at 37 degrees C. 3. The inhibitory effect of ChH was dose-dependent and was accompanied by an increase of microviscosity as measured by a fluorescence polarization technique with the probe diphenylhexatriene. 4. Experiments with increasing concentrations of isoproterenol indicated that the efficiency, but not the potency, of the beta-adrenergic response was affected by the increasing of the cholesterol content in the cell membrane.
Prieto
J C
JC
Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá, Spain.
Hueso
C
C
Carmena
M J
MJ
D000818:Animals; D002788:Cholesterol Esters / Q000494:pharmacology; D000242:Cyclic AMP / Q000737:chemistry / Q000378:metabolism*; D004847:Epithelial Cells; D008297:Male; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000494:pharmacology; D011467:Prostate / Q000166:cytology*; D051381:Rats; D011919:Rats, Inbred Strains; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*
D002788:Cholesterol Esters; D008563:Membrane Lipids; D011943:Receptors, Adrenergic, beta; D000242:Cyclic AMP; C013440:cholesteryl succinate