Role of soluble cytokines on the restricted replication of poliovirus in the monocytic U937 cell line.
Poliovirus infection of monocytic U937 cells is characterized by an incomplete shut-off of host protein synthesis occurring at late times postinfection and by a slow accumulation of viral mRNA which is not efficiently translated. By using exogenous cytokines, neutralizing antibodies to different cytokines and specific cDNA probes, we have studied the involvement of soluble factors on the restricted replication of poliovirus in U937 cells. Our results show that both IFN-gamma and TNF-alpha mRNA levels increased in U937 cells upon poliovirus infection. Moreover, treatment of infected U937 cells with antibodies to IFN-gamma induced a remarkably stronger shut-off of host protein synthesis than that found in control cultures. A role of IFN-gamma in protecting the infected cells from an extensive blockade of host mRNA translation is proposed.
López-Guerrero
J A
JA
Centro de Biología Molecular, Universidad Autónoma, CSIC, Madrid, Spain.
Pimentel-Muiños
F X
FX
Fresno
M
M
Alonso
M A
MA
D001685:Biological Factors / Q000494:pharmacology*; D002460:Cell Line; D016207:Cytokines; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D009000:Monocytes / Q000382:microbiology*; D011051:Poliomyelitis / Q000188:drug therapy / Q000235:genetics; D017955:Poliovirus / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D012333:RNA, Messenger / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects*
D001685:Biological Factors; D016207:Cytokines; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma