In vitro synthesis of nitroxide free radicals by hog liver microsomes.
The in vitro biooxidation of 4-hydroxy-2,2,6,6-tetramethylpiperidine (TEMP), 4-hydroxy-2,2,4,4-tetramethyl-1,3-oxazolidine (TEMO) and diphenylamine (DPA) by hog liver microsomes to their respective nitroxide free radicals, 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO), 2,2,4,4-tetramethyl-1,3-oxazolidine-1-oxyl (TEMOO), and diphenylnitroxide (DPNO) has been investigated. For extending the life span of the liver microsomes, a calcium alginate immobilization procedure was used. The biooxidation rates of the above amines to their respective nitroxide metabolites were measured by means of oxygen uptake at 37 degrees C and pH 7.4. N-octylamine was found to be an activator in the biooxidation of the amines. The formation of the nitroxide radicals was identified by E.S.R. spectroscopy.
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N.J.I.T. Biotechnology Laboratory, New Jersey Institute of Technology, Newark 07102.
Lischick
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Sofer
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D000464:Alginates / Q000494:pharmacology; D000588:Amines / Q000494:pharmacology; D000818:Animals; D003497:Cyclic N-Oxides / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D009589:Nitrogen Oxides / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D013552:Swine
D000464:Alginates; D000588:Amines; D003497:Cyclic N-Oxides; D005609:Free Radicals; D006603:Hexuronic Acids; D009589:Nitrogen Oxides; D020723:Glucuronic Acid; C039900:nitroxyl; D010100:Oxygen; C008699:octylamine; C003959:TEMPO