Nucleotide sequence of the region encompassing the int gene of a cryptic prophage and the dna Y gene flanked by a curved DNA sequence of Escherichia coli K12.
The nucleotide sequence of a 2.5 kb region encompassing a curved DNA segment (BENT-9) randomly cloned from the total Escherichia coli chromosome was determined. This region was found to contain the dna Y gene encoding a transfer RNA. The curved DNA structure was demonstrated to be located just upstream of the dna Y promoter. The results of sequencing further revealed that the int gene of a cryptic prophage, qsr', which has been shown to be present in the E. coli genome, is located next to the dna Y gene.
Muramatsu
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S
Laboratory of Microbiology, School of Agriculture, Nagoya University, Japan.
Mizuno
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T
D000595:Amino Acid Sequence; D001483:Base Sequence; D003090:Coliphages / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005814:Genes, Viral; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012343:RNA, Transfer / Q000235:genetics*; D015183:Restriction Mapping
D004269:DNA, Bacterial; D012343:RNA, Transfer