Adjuvanticity of recombinant bovine interleukin-1 beta: influence on immunity, infection, and latency in a bovine herpesvirus-1 infection.
Recombinant bovine interleukin-1 beta (rBoIL-1 beta) was administered to calves in conjunction with a bovine herpesvirus-1 (BHV-1) vaccine. All calves were immunized against BHV-1 and three groups received rBoIL-1 beta at 33, 100, or 330 ng/kg on days 1 and 15; control animals received physiological saline. All calves were challenged with BHV-1 on day 22. Total leukocytes were increased by rBoIL-1 beta, primarily by causing neutrophilia and monocytosis; CD4/CD8 ratios tended to be increased in rBoIL-1 beta-treated animals. Serum neutralizing antibody titers and cytotoxic responses against BHV-1-infected bovine kidney fibroblasts were increased and virus excretion was decreased in rBoIL-1 beta-treated calves. On days 58 and 59, control and 100 ng/kg rBoIL-1 beta-treated calves were injected with dexamethasone (.04 mg/kg). Virus excretion was less and clinical signs of BHV-1 infection were lower in rBoIL-1 beta-treated calves after dexamethasone injection. These data suggest that rBoIL-1 beta may be an effective adjuvant to BHV-1 immunization.
Reddy
D N
DN
Department of Anatomy, Kansas State University, Manhattan.
Reddy
P G
PG
Minocha
H C
HC
Fenwick
B W
BW
Baker
P E
PE
Davis
W C
WC
Blecha
F
F
D000276:Adjuvants, Immunologic; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle; D003602:Cytotoxicity, Immunologic; D003907:Dexamethasone / Q000494:pharmacology; D007242:Herpesvirus 1, Bovine / Q000276:immunology / Q000302:isolation & purification; D007241:Infectious Bovine Rhinotracheitis / Q000097:blood / Q000276:immunology / Q000517:prevention & control*; D007375:Interleukin-1 / Q000008:administration & dosage / Q000494:pharmacology*; D007501:Iron / Q000097:blood; D007958:Leukocyte Count; D008297:Male; D009297:Nasal Mucosa / Q000382:microbiology; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D007375:Interleukin-1; D011994:Recombinant Proteins; D014765:Viral Vaccines; D003907:Dexamethasone; D007501:Iron