title: Beta-subunit of bovine rod photoreceptor cGMP phosphodiesterase. Comparison with the phosphodiesterase family.
abstract: A group of cDNA clones encoding the beta-subunit of bovine rod photoreceptor cGMP phosphodiesterase were isolated for structural analysis. The encoded polypeptide has 853 residues with a calculated molecular mass of 98 kDa. The beta-subunit is 72% identical to the rod cGMP phosphodiesterase alpha-subunit. Like the alpha-subunit and the cone alpha'-subunit, the beta-subunit belongs to the family of phosphodiesterase genes. The beta- and alpha-subunits are more similar to each other than either is to the cone alpha'-subunit, suggesting either that the beta- and alpha-subunits diverged more recently or that their divergence was restrained by the rod functional environment.
authors.affiliation: Shemyakin Institute of Bioorganic Chemistry, Union of Soviet Socialist Republics Academy of Sciences, Moscow.
authors.forename: V M
authors.initials: VM
authors.lastname: Lipkin
authors.forename: N V
authors.initials: NV
authors.lastname: Khramtsov
authors.forename: I A
authors.initials: IA
authors.lastname: Vasilevskaya
authors.forename: N V
authors.initials: NV
authors.lastname: Atabekova
authors.forename: K G
authors.initials: KG
authors.lastname: Muradov
authors.forename: V V
authors.initials: VV
authors.lastname: Gubanov
authors.forename: T
authors.initials: T
authors.lastname: Li
authors.forename: J P
authors.initials: JP
authors.lastname: Johnston
authors.forename: K J
authors.initials: KJ
authors.lastname: Volpp
authors.forename: M L
authors.initials: ML
authors.lastname: Applebury
mesh_terms: D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004247:DNA / Q000235:genetics; D015723:Gene Library; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D008970:Molecular Weight; D005810:Multigene Family; D015345:Oligonucleotide Probes; D010727:Phosphoric Diester Hydrolases / Q000235:genetics*; D010786:Photoreceptor Cells / Q000201:enzymology*; D015183:Restriction Mapping; D012374:Rod Cell Outer Segment / Q000201:enzymology*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D046911:Macromolecular Substances; D015345:Oligonucleotide Probes; D004247:DNA; D010727:Phosphoric Diester Hydrolases; D015106:3',5'-Cyclic-GMP Phosphodiesterases