A tandem duplication causes the Kn1-O allele of Knotted, a dominant morphological mutant of maize.
Molecular and genetic techniques are used to define Kn1-O, a mutation which interferes with the normal differentiation of vascular tissue in leaves. Sequences associated with a previously cloned allele, Kn1-2F11, were used as hybridization probes in a Southern analysis of Kn1-O. By this analysis, Kn1-O lacks the Ds2 transposable element that causes Kn1-2F11 but instead is associated with a sequence duplication. Sequence and restriction analysis of genomic clones show that the duplication consists of a tandem array of two 17-kb repeats. Analysis of Kn1-O derivatives indicates that the duplication itself conditions the mutant phenotype; a severely knotted line, Kn1-Ox, has gained a repeat unit to form a triplication, whereas normal derivatives have either lost a repeat unit or sustained insertions that disrupt the tandem duplication. These insertions map near the central junction of the tandem duplication, suggesting that the mutant phenotype results from the novel juxtaposition of sequences. We discuss models that relate the tandem duplication of sequences to altered gene expression.
Veit
B
B
Plant Gene Expression Center, U.S. Department of Agriculture/University of California, Albany 94710.
Vollbrecht
E
E
Mathern
J
J
Hake
S
S
D000483:Alleles; D001483:Base Sequence; D015139:Blotting, Southern; D004251:DNA Transposable Elements; D005799:Genes, Dominant; D008969:Molecular Sequence Data; D005810:Multigene Family; D009154:Mutation; D010641:Phenotype; D015183:Restriction Mapping; D003313:Zea mays / Q000235:genetics*
D004251:DNA Transposable Elements