title: Comparison of the detection of cervical human papillomavirus infection by filter DNA hybridization of cytologic specimens and by in situ DNA hybridization of tissues.
abstract: Cellular samples and subsequent cone biopsy samples from the same site in 18 patients were screened for infection with human papillomavirus (HPV) types 16 and 18 (HPV 16/18) by DNA hybridization. Filter hybridization of cells collected using cervical swabs was significantly less sensitive (with only 4 positive results) in detecting HPV 16/18 DNA sequences than was in situ hybridization of tissue sections (with 16 positive results). The in situ hybridization results correlated well with the cytologic and histologic findings of cervical intraepithelial neoplasia of grades II (mild dysplasia) and III (severe dysplasia and carcinoma in situ).
authors.affiliation: Institute of Virology, University of Köln, Federal Republic of Germany.
authors.forename: R
authors.initials: R
authors.lastname: Neumann
authors.forename: G
authors.initials: G
authors.lastname: Nelles
authors.forename: H J
authors.initials: HJ
authors.lastname: Eggers
authors.forename: S
authors.initials: S
authors.lastname: Diehl
authors.forename: H
authors.initials: H
authors.lastname: Cremer
authors.forename: H K
authors.initials: HK
authors.lastname: Zinser
mesh_terms: D001345:Autoradiography; D001706:Biopsy; D002584:Cervix Uteri / Q000166:cytology / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D014412:Tumor Virus Infections / Q000175:diagnosis*; D002583:Uterine Cervical Neoplasms / Q000382:microbiology
chemical_list: D004279:DNA, Viral