BIO-Fully Automated Sample Treatment high-performance liquid chromatography and radioimmunoassay for leukotriene E4 in human urine from asthmatics.
BIO-Fully Automated Sample Treatment (BIO-FAST) high-performance liquid chromatography (HPLC) is a sophisticated column-switching technique in which a fresh pre-column is used for each sample prior to reversed-phase HPLC. The pre-columns, Varian Advanced Automated Sample Processor (AASP) cartridges, are held and automatically advanced by the Varian AASP. A rapid and efficient extraction and separation for leukotrienes C4 and E4 from human urine has been developed using a C8 cartridge and subsequent C18 analytical HPLC column. Quantitation of leukotriene E4, accomplished by post-column radioimmunoassay, shows significantly increased leukotriene E4 concentrations in urine samples from asthmatics after antigen challenge. This further confirms an active role for leukotrienes in the pathogenesis of bronchial asthma.
Nicoll-Griffith
D
D
Department of Medicinal Chemistry, Merck Frosst Centre for Therapeutic Research, Pointe Claire-Dorval, Quebec, Canada.
Zamboni
R
R
Rasmussen
J B
JB
Ethier
D
D
Charleson
S
S
Tagari
P
P
D001249:Asthma / Q000652:urine*; D001322:Autoanalysis; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D006801:Humans; D017999:Leukotriene E4; D011863:Radioimmunoassay; D013189:SRS-A / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000652:urine
D013189:SRS-A; D017999:Leukotriene E4