Influence of ciprofloxacin on leukotriene generation from various cells in vitro.
The effect of ciprofloxacin on leukotriene generation from human polymorphonuclear leucocytes (PMN) and the respective lymphocyte, monocyte and basophil (LMB) containing cell fraction has been studied. Furthermore, the influence on the LTB4-receptor expression of PMNL, as well as on the synthesis of 12-hydroxyeicosatetraenoic acid (12-HETE) from human platelets, was analysed. The antibiotic concentrations ranged from 0.5 to 4 micrograms/10(7) cells. Analysis of the generated leukotrienes was performed by high performance liquid chromatography (HPLC). The calcium-ionophore A23187 induced leukotriene generation from PMNs and LMBs was significantly suppressed by preincubation with ciprofloxacin in a dose-dependent manner. Incubation of PMNs with the same concentrations of ciprofloxacin induced an augmentation of the LTB4-receptor expression. Preincubation of human platelets led to a suppression of the calcium-ionophore induced 12-HETE generation at high concentrations (8 and 4 micrograms/10(8) cells) and to an increased synthesis of 12-HETE at lower concentrations (0.5, 1, and 2 micrograms ciprofloxacin/1 x 10(8) platelets).
Knöller
J
J
Lehrstuhl für Medizinische Mikrobiologie und Immunologie, Ruhr Universität Bochum, FRG.
Brom
J
J
Schönfeld
W
W
König
W
W
D001773:Blood Cells / Q000187:drug effects* / Q000378:metabolism; D001792:Blood Platelets / Q000187:drug effects; D000001:Calcimycin / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D002939:Ciprofloxacin / Q000494:pharmacology*; D006098:Granulocytes / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D007975:Leukotriene B4 / Q000096:biosynthesis* / Q000097:blood; D009000:Monocytes / Q000187:drug effects; D010084:Oxidation-Reduction
D007975:Leukotriene B4; D000001:Calcimycin; D002939:Ciprofloxacin