A clinically applicable method to preserve urine and bladder washing cells for flow cytometric monitoring of bladder cancer patients.
We describe a method to fix exfoliated bladder cells that is suitable for followup of bladder cancer patients by deoxyribonucleic acid flow cytometry. After fixation with room temperature methanol plus acetic acid (20:1, volume:volume) urine and bladder washing samples from these patients can be stored at room temperature for 3 to 7 days and then assessed reliably for the presence of aneuploidy and the percentage of hyperdiploid cells. For those with active transitional cell carcinoma diagnostic accuracy comparing fresh to fixed specimens was improved from 58 to 92% with urine and from 50 to 100% with washing samples. For patients with a history of transitional cell carcinoma who currently are free of disease the false positive rate remains unchanged after fixation. The procedure described is suitable for use in the outpatient clinic and should permit shipping of samples without refrigeration to a central flow cytometry facility for analysis.
Deitch
A D
AD
Department of Urology, University of California, Davis, Sacramento.
Andreotti
V A
VA
Strand
M A
MA
Howell
L
L
deVere White
R W
RW
D002295:Carcinoma, Transitional Cell / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005404:Fixatives; D005434:Flow Cytometry; D006652:Histological Techniques; D006801:Humans; D008297:Male; D011003:Ploidies; D007507:Therapeutic Irrigation; D001743:Urinary Bladder / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D014556:Urine / Q000166:cytology
D004273:DNA, Neoplasm; D005404:Fixatives