Expression, partial purification and immunogenicity of fragments of the knob protein of Plasmodium falciparum.
Three structural domains of the histidine-rich knob protein (KP) of Plasmodium falciparum were expressed in Escherichia coli. A single-step purification scheme was devised to obtain great enrichment of expressed polypeptides for use in subsequent experiments. Immune human sera from Africa, South-East Asia and South America were tested for reactivity with each of the expressed fragments. While the two fragments which represented the central and C-terminal regions of KP showed a strong reactivity with all the antisera which were tested, the N-terminal fragment which contains the repetitive histidine-rich sequences showed almost no reactivity.
Rashid
M A
MA
Public Health Research Institute, New York, NY 10016.
Yang
Y F
YF
Kilejian
A
A
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002852:Chromatography, Ion Exchange; D006639:Histidine; D008565:Membrane Proteins / Q000096:biosynthesis / Q000276:immunology*; D008969:Molecular Sequence Data; D010452:Peptide Biosynthesis; D010446:Peptide Fragments / Q000276:immunology / Q000302:isolation & purification; D010455:Peptides / Q000276:immunology* / Q000302:isolation & purification; D010963:Plasmodium falciparum / Q000276:immunology*; D015800:Protozoan Proteins / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification
D008565:Membrane Proteins; D010446:Peptide Fragments; D010455:Peptides; D015800:Protozoan Proteins; D011994:Recombinant Proteins; C042968:knob protein, Plasmodium falciparum; D006639:Histidine