Non-NMDA receptor mediates cytoplasmic Ca2+ elevation in cultured hippocampal neurones.
Microscopic fluorometry of cultured rat hippocampal neurones revealed that the intracellular concentration of Ca2+ ([Ca2+]i) rises in response to the activation of excitatory amino acid receptor (EAA-R), which included not only N-methyl-D-aspartate subspecies but also kainate and quisqualate subspecies of EAA-R. Each EAA-R mediated [Ca2+]i rise consisted of the components dependent on and independent of the activity of the voltage-dependent Ca2+ channel. The receptor-mediated voltage-independent [Ca2+]i rise may be related to the modulation of synaptic transmission efficacy.
Ogura
A
A
Department of Neuroscience, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Akita
K
K
Kudo
Y
Y
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism; D002478:Cells, Cultured; D003470:Culture Media; D003593:Cytoplasm / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D007608:Kainic Acid / Q000494:pharmacology; D016202:N-Methylaspartate / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D016318:Quisqualic Acid / Q000494:pharmacology; D051381:Rats; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface / Q000502:physiology*; D012964:Sodium / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013569:Synapses / Q000502:physiology; D009435:Synaptic Transmission / Q000502:physiology
D015220:Calcium Channels; D003470:Culture Media; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface; D016202:N-Methylaspartate; D011189:Potassium Chloride; D016318:Quisqualic Acid; D012964:Sodium; D007608:Kainic Acid; D002118:Calcium