Receptors for monoamines on cultured cells.
Clonal cell lines are ideal model systems for the study of monoamine receptors. Cell lines that express dopamine and serotonin receptors have been isolated from pituitary tumor 7315a. The SUP1 cell line contains mRNA for the dopamine D-2 receptor and expresses D-2 receptors coupled to the inhibition of cAMP production and to the inhibition of prolactin secretion. The P11 cell line expresses 5-HT2 receptors coupled to the stimulation of phosphoinositide hydrolysis. These cell lines will be used to investigate manipulations that affect the expression and function of dopamine and serotonin receptors.
Ivins
K J
KJ
Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104-6084.
Molinoff
P B
PB
D000067956:Adenylyl Cyclase Inhibitors; D000818:Animals; D001921:Brain / Q000378:metabolism; D003470:Culture Media; D000242:Cyclic AMP / Q000037:antagonists & inhibitors; D006868:Hydrolysis; D008238:Lysergic Acid Diethylamide; D010716:Phosphatidylinositols / Q000378:metabolism; D010903:Pituitary Gland, Anterior / Q000378:metabolism; D011388:Prolactin / Q000378:metabolism; D011954:Receptors, Dopamine / Q000378:metabolism* / Q000502:physiology; D017448:Receptors, Dopamine D2; D011985:Receptors, Serotonin / Q000378:metabolism*; D012701:Serotonin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000067956:Adenylyl Cyclase Inhibitors; D003470:Culture Media; D010716:Phosphatidylinositols; D011954:Receptors, Dopamine; D017448:Receptors, Dopamine D2; D011985:Receptors, Serotonin; D012701:Serotonin; D008238:Lysergic Acid Diethylamide; D011388:Prolactin; D000242:Cyclic AMP