title: NMR investigation of cell cultures: early detection of infection by Herpes simplex virus type 2 and transformation.
abstract: The water proton Nuclear Magnetic Resonance (NMR) spin-lattice relaxation rates were measured in suspension of uninfected and Herpes simplex virus type 2-infected WI-38 diploid cells and shown to allow reliable detection of (i) the early signs of productive infection, and (ii) the tendency to undergo either spontaneous or virus-induced transformation. In addition, NMR investigation of several transformed cell lines currently and previously established from WI-38 cells clearly indicated that the NMR relaxation parameter is a suitable tool for assessment of the level of malignancy, as revealed by in vivo oncogenicity tests in immunosuppressed mice.
authors.affiliation: Istituto di Microbiologia, Università di Siena, Italy.
authors.forename: P E
authors.initials: PE
authors.lastname: Valensin
authors.forename: M L
authors.initials: ML
authors.lastname: Bianchi Bandinelli
authors.forename: M
authors.initials: M
authors.lastname: Zazzi
authors.forename: E
authors.initials: E
authors.lastname: Gaggelli
authors.forename: G
authors.initials: G
authors.lastname: Valensin
mesh_terms: D000818:Animals; D002460:Cell Line; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D003588:Cytopathogenic Effect, Viral; D004171:Diploidy; D005347:Fibroblasts; D006801:Humans; D007165:Immunosuppression Therapy; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D018139:Simplexvirus / Q000302:isolation & purification* / Q000502:physiology