6DMAP inhibits chromatin decondensation but not sperm histone kinase in sea urchin male pronuclei.
Treatment of sea urchin eggs for 10 min prior to fertilization with the kinase inhibitor 6DMAP (6-dimethylaminopurine) reversibly inhibits swelling and loss of conical morphology of the male pronucleus. Male pronuclei inhibited with 1 mM 6DMAP for 25 min undergo phosphorylation of Sp H1 and Sp H2B histones as fully as do control nuclei. Therefore, Sp histone kinase, whose target sequences resemble those of the M-phase histone kinase, is not inhibited by 6DMAP, and Sp histone phosphorylation, although it may be necessary, is not sufficient for chromatin decondensation.
Poccia
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Department of Biology, Amherst College, Massachusetts 01002.
Pavan
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Green
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GR
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects* / Q000648:ultrastructure; D002843:Chromatin / Q000187:drug effects* / Q000378:metabolism; D005306:Fertilization; D006657:Histones / Q000378:metabolism; D008297:Male; D010766:Phosphorylation; D006656:Protamine Kinase / Q000037:antagonists & inhibitors*; D047428:Protein Kinase Inhibitors; D012617:Sea Urchins; D015053:Zygote / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure
D002843:Chromatin; D006657:Histones; D047428:Protein Kinase Inhibitors; C001020:N(6),N(6)-dimethyladenine; D006656:Protamine Kinase; D000225:Adenine