Heterotypic protection induced by synthetic peptides corresponding to three serotypes of foot-and-mouth disease virus.
Synthetic peptide vaccines of the general sequence Cys-Cys(200-213)-Pro-Pro-Ser-(141-158)-Pro-Cys-Gly, where the numbered residues refer to VP1 sequences of three different strains of foot-and-mouth disease virus, have been evaluated in cattle and guinea pigs. High levels of serotype-specific (homotypic) antiviral and antipeptide antibody were produced with each peptide. The A- and O-serotype peptides provided complete protection of guinea pigs against their respective virus challenges. The C-serotype peptide appeared to be less effective than the other peptides. In cross-protection studies (heterotypic) in guinea pigs, it was possible to protect A-serotype peptide-vaccinated animals against O-virus challenge and vice versa. Some heterotypic protection was also achieved with the C-serotype peptide. The heterotypic protection observed related more to the presence of cross-reactive antipeptide antibody than to neutralizing antibody.
Doel
T R
TR
Agricultural and Food Research Council Institute for Animal Health, Pirbright Laboratory, Woking, United Kingdom.
Gale
C
C
Do Amaral
C M
CM
Mulcahy
G
G
Dimarchi
R
R
D000595:Amino Acid Sequence; D000818:Animals; D000910:Antibodies, Heterophile / Q000032:analysis*; D000914:Antibodies, Viral / Q000032:analysis; D005537:Aphthovirus / Q000145:classification / Q000235:genetics / Q000276:immunology*; D002417:Cattle; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000517:prevention & control*; D006168:Guinea Pigs; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis*; D012703:Serotyping; D014611:Vaccination; D014612:Vaccines / Q000008:administration & dosage*; D014614:Vaccines, Synthetic / Q000008:administration & dosage*; D014765:Viral Vaccines / Q000008:administration & dosage*
D000910:Antibodies, Heterophile; D000914:Antibodies, Viral; D010455:Peptides; D014612:Vaccines; D014614:Vaccines, Synthetic; D014765:Viral Vaccines