Antiviral effects of 2',5'oligoadenylates (2-5As), and related compounds.
Dephosphorylated "core" of 2',5'-oligoadenylate (2-5A) dimer (A2'p5'A), exogenously added to nonpermeabilized FL cells, inhibited the multiplication of Sindbis virus and vesicular stomatitis virus (VSV). The compound was shown to inhibit viral protein synthesis. The addition of A2'p5'A at the early stage of viral replication was more effective than that at the late stage. In contrast with the core, phosphorylated 2-5A (p5'A2'p5'A and ppp5'A2'p5'A) and 2-5A analogs containing cordycepin (3'-deoxyadenosine) did not show such antiviral effects. The rate of uptake of [3H]ppp5'A 2'p5'A into acid-soluble and acid-insoluble fractions, especially into the acid-insoluble fraction, was faster than that of [3H]A2'p5'A. These results suggest that the difference of antiviral activity between A2'p5'A and ppp5'A2'p5'A does not result from the different rate of uptake by cells, but from the different rate of from acid-soluble to acid-insoluble fractions.
Tominaga
A
A
Tokyo Research Institute, Seikagaku Kogyo Co., Ltd.
Saito
S
S
Kohno
S
S
Sakurai
K
K
Hayakawa
Y
Y
Noyori
R
R
D000227:Adenine Nucleotides / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D000998:Antiviral Agents / Q000138:chemical synthesis; D002478:Cells, Cultured; D002642:Chick Embryo; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D003839:Deoxyadenosines / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D009843:Oligoribonucleotides / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D010766:Phosphorylation; D012845:Sindbis Virus / Q000187:drug effects* / Q000254:growth & development; D013997:Time Factors; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects* / Q000254:growth & development; D014764:Viral Proteins / Q000187:drug effects; D014776:Virus Cultivation; D014779:Virus Replication / Q000187:drug effects
D000227:Adenine Nucleotides; D000998:Antiviral Agents; D003839:Deoxyadenosines; D009843:Oligoribonucleotides; D014764:Viral Proteins; C023505:2',5'-oligoadenylate; C058120:cordycepin