[Isotope study of in vitro K(+) uptake and release of erythrocytes in juvenile diabetes with 86Rb].
In order to determine the reasons for a previously observed elevated intracellular Na+ and a decreased K+ level, the K+ uptake and release of the red blood cells in type I diabetics were examined with 86Rb. The K+ release and the ouabain-insensitive K+ uptake were different from those in the controls. Under the present experimental conditions, there was no decrease in ouabain-sensitive K+ uptake indicative of the activity of K+, Na(+)-ATPase. Likewise, an elevated activity normalizing a lower K+ level could not be detected. Further, there were no significant differences in the reaction kinetic parameters: KM[diab.]:4.21 +/- 1.38 mM/l, KM[contr.]:4.14 +/- 0.99 mM/l Vmax[diab.]:2259 +/- 326 nmol/ml red blood cell/hour Vmax[contr.]:2354 +/- 462 nmol/ml red blood cell/hour. These alterations are regarded as partial phenomena of the complex membrane function disturbances in diabetic red blood cells.
Román
F
F
Kinderkrankenhaus, Poliklinik der Stadt Szeged, Ungarische Republik.
Sánta
A
A
Rimanóczky
A
A
Toldi
Z
Z
Pataki
L
L
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D003922:Diabetes Mellitus, Type 1 / Q000201:enzymology*; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D006801:Humans; D008297:Male; D011188:Potassium / Q000097:blood*; D012414:Rubidium Radioisotopes; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood*
D012414:Rubidium Radioisotopes; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium
Isotopische Untersuchung der in vitro-K(+)-Aufnahme und -Abgabe der Erythrozyten beim juvenilen Diabetes mit 86Rb.