Retinal cyclic GMP and AMP in rod-cone dysplasia: comparison of assays in fixed and unfixed retinal tissues.
The effects of 3 fixatives (formalin, Bouin's solution and glutaraldehyde-paraformaldehyde) on retinal cyclic AMP and GMP content were determined in canine eyes with a known abnormality of cyclic GMP metabolism. Cyclic nucleotides were measured by radioimmunoassay on perchloric acid (PCA)-precipitated retinal samples from fixed (left) and unfixed (right) eyes from nine 6-week-old rod-cone-dysplasia-affected Irish setter dogs. Cyclic AMP values were significantly lower for fixed retinas than for unfixed controls. Retinal cyclic GMP values were also lower from glutaraldehyde-paraformaldehyde-fixed samples, but were not different from control values for formalin- or Bouin's-fixed eyes, when the retina was PCA-precipitated promptly after fixation.
Acland
G M
GM
Department of Ophthalmology, School of Medicine, University of Pennsylvania, Philadelphia.
Fletcher
R T
RT
Chader
G J
GJ
Aguirre
G D
GD
D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D004285:Dogs / Q000002:abnormalities*; D005404:Fixatives; D006652:Histological Techniques; D010786:Photoreceptor Cells / Q000473:pathology*; D012164:Retinal Diseases / Q000201:enzymology*
D005404:Fixatives; D000242:Cyclic AMP; D006152:Cyclic GMP