Protein kinase C activity in erythrocytes in primary hypertension: regulation of cell shape and cation transport.
Protein kinase C activity in the lysate of erythrocytes of patients with essential hypertension (EH) and spontaneously hypertensive rats (SHR) was found to be increased by 1.6-2.0 times as compared with normotensive controls. Membrane cytoskeleton alterations observed in the erythrocytes of patients with EH and SHR were revealed in decreased average erythrocyte volume, increase of cup-shaped cell formation, and increase of basal phosphorylation of band 4.9 protein. In addition, the rate of Na(+)-H+ exchange in erythrocytes of EH patients and SHR was increased by 1.9-fold. In vitro treatment of erythrocytes of healthy donors and Wistar-Kyoto rats (WKY) with protein kinase C activator (12-O-tetradecanoylphorbol-13-acetate) leads to similar changes of cell shape, cell volume, band 4.9 protein phosphorylation and Na(+)-H+ exchange, as well as to an increase of diS-C3-(5) fluorescence. It may be assumed that alterations of these parameters revealed in primary hypertension are caused by increased activity of protein kinase C.
Kravtsov
G M
GM
Central Research Laboratory of the Ministry of Public Health of the USSR, Moscow.
Dulin
N O
NO
Postnov IYu
Orlov
S N
SN
Pokudin
N I
NI
Kotelevtsev YuV
Postnov YuV
D000328:Adult; D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism; D004911:Erythrocyte Volume; D004912:Erythrocytes / Q000166:cytology / Q000201:enzymology*; D006801:Humans; D006973:Hypertension / Q000097:blood / Q000201:enzymology*; D006977:Hypertension, Renal / Q000097:blood / Q000201:enzymology*; D007474:Ion Exchange; D008094:Lithium / Q000378:metabolism; D008297:Male; D008564:Membrane Potentials; D008875:Middle Aged; D010766:Phosphorylation; D011493:Protein Kinase C / Q000097:blood*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012964:Sodium / Q000097:blood / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D002352:Carrier Proteins; D017923:Sodium-Hydrogen Exchangers; D008094:Lithium; D012964:Sodium; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate