Regulation of cGMP production by intracellular alkalinization in cultured rat inner medullary collecting duct cells.
We evaluated the relationship between cell pH and cGMP production in cultured rat renal inner medullary collecting duct cells. The cGMP level, 21 +/- 6, was not different in control vs. alkalinized cells, 49 +/- 17 fmol/mg protein (p greater than 0.5). 10(-11) M atrial natriuretic peptide (ANF) enhanced cGMP production in alkalinized cells, 426 +/- 34 vs. 141 +/- 9*. Conversely, alkalinization inhibited 10(-4)M nitroprusside (SNP) induced cGMP formation, 29 +/- 9 vs. 332 +/- 67*. Phosphodiesterase inhibition abolished the difference in cGMP production by ANF but did not reverse the inhibitory effect of alkalinization on SNP induced cGMP production. In rat renal inner medullary collecting duct cells, cellular alkalinization plays a significant role in the regulation of guanylate cyclase mediated cGMP production. * = p less than 0.05).
Siskind
M S
MS
Thorndike Memorial Laboratory, Renal Section, Boston City Hospital, MA 02118.
Alexander
E A
EA
Schwartz
J H
JH
D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D002478:Cells, Cultured; D006152:Cyclic GMP / Q000096:biosynthesis*; D003600:Cytosol; D005292:Ferricyanides / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007679:Kidney Medulla / Q000187:drug effects / Q000378:metabolism*; D007684:Kidney Tubules / Q000378:metabolism*; D007685:Kidney Tubules, Collecting / Q000187:drug effects / Q000378:metabolism*; D009550:Nigericin / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology*; D051381:Rats
D005292:Ferricyanides; D000643:Ammonium Chloride; D009599:Nitroprusside; D009320:Atrial Natriuretic Factor; D006152:Cyclic GMP; D009550:Nigericin