title: Studies on the structure and regulation of the human hepatic interleukin-6 receptor.
abstract: Affinity cross-linking of 125I-labeled recombinant human interleukin-6 (IL-6) to human hepatoma cells (HepG2) allowed the detection of three IL-6-containing complexes with molecular masses of 100 kDa, 120 kDa and 200 kDa. Treatment of HepG2 cells with dexamethasone led to a time- and dose-dependent up-regulation of IL-6-receptor mRNA levels. By the use of cross-linking this effect was also seen at the protein level, where all three IL-6-binding complexes increased upon incubation of HepG2 cells with dexamethasone. Under conditions of IL-6-receptor up-regulation by dexamethasone, gamma-fibrinogen mRNA induction by IL-6 is stronger and occurs earlier than without dexamethasone. We propose therefore that the expression of the IL-6 receptor might be a rate-limiting step in acute-phase-protein induction.
authors.affiliation: Institut für Biochemie der Rheinisch-Westfälischen Technischen Hochschule Aachen, Federal Republic of Germany.
authors.forename: S
authors.initials: S
authors.lastname: Rose-John
authors.forename: H
authors.initials: H
authors.lastname: Schooltink
authors.forename: D
authors.initials: D
authors.lastname: Lenz
authors.forename: E
authors.initials: E
authors.lastname: Hipp
authors.forename: G
authors.initials: G
authors.lastname: Dufhues
authors.forename: H
authors.initials: H
authors.lastname: Schmitz
authors.forename: X
authors.initials: X
authors.lastname: Schiel
authors.forename: T
authors.initials: T
authors.lastname: Hirano
authors.forename: T
authors.initials: T
authors.lastname: Kishimoto
authors.forename: P C
authors.initials: PC
authors.lastname: Heinrich
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology*; D005340:Fibrinogen / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D052138:Genes, Neoplasm; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology; D008113:Liver Neoplasms / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000187:drug effects* / Q000235:genetics; D019947:Receptors, Interleukin-6; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D015850:Interleukin-6; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D019947:Receptors, Interleukin-6; D011994:Recombinant Proteins; D003907:Dexamethasone; D005340:Fibrinogen