title: Primary structure of the S peplomer gene of bovine coronavirus and surface expression in insect cells.
abstract: The nucleotide sequence of the S peplomer gene of bovine coronavirus (BCV) has been determined. A single open reading frame of 4089 nucleotides encodes a polypeptide of 150K with 20 potential sites for addition of N-linked oligosaccharides. Expression of the cloned BCV S gene by a recombinant of Autographa californica nuclear polyhedrosis virus resulted in production of a 180K glycosylated polypeptide which was transported to the surface of the cell. Comparison of the BCV S gene with the analogous genes of murine hepatitis viruses shows that the BCV S polypeptide contains a unique domain of 138 amino acids not present in murine hepatitis virus strain JHM, but which has a partially homologous counterpart in strain A59. This domain accounts for most of the differences in size of the S gene products of these coronaviruses.
authors.affiliation: Veterinary Infectious Disease Organization, Saskatoon, Saskatchewan, Canada.
authors.forename: M D
authors.initials: MD
authors.lastname: Parker
authors.forename: D
authors.initials: D
authors.lastname: Yoo
authors.forename: G J
authors.initials: GJ
authors.lastname: Cox
authors.forename: L A
authors.initials: LA
authors.lastname: Babiuk
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D003332:Coronaviridae / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D015967:Gene Expression Regulation, Viral; D006023:Glycoproteins / Q000235:genetics*; D007304:Insect Viruses / Q000235:genetics; D008969:Molecular Sequence Data; D009036:Moths; D006517:Murine hepatitis virus / Q000235:genetics; D011233:Precipitin Tests; D012367:RNA, Viral / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D006023:Glycoproteins; D012367:RNA, Viral; D014759:Viral Envelope Proteins