Tumor cell implantation with the use of Gelaspon gelatin sponge disc.
Myelomonocytic leukemia (My) mesoblastic nephroma (Ne) and hepatocellular carcinoma (He) cells were implanted under the renal capsule of F344, Long-Evans (LE) and BDIX rats. Gelaspon sponge discs were used in the implantation procedure, which were resorbed within a few days. The tumor cells, which were located on the surface of these discs could then attach themselves to the renal capsule and thus grow. There was a correlation between the number of tumor cells and the difference between the two kidney masses. The correlation was linear between 10(4) and 10(6) cells, thus the method proved to be a simple, fast and quantitative model in experimental cancer prevention and therapy.
Uzvolgyi
E
E
Department of Hygiene and Epidemiology, University Medical School of Debrecen, DOTE, Hungary.
Katona
A
A
Kertai
P
P
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D002452:Cell Count; D005260:Female; D005781:Gelatin Sponge, Absorbable; D007680:Kidney Neoplasms / Q000473:pathology; D007951:Leukemia, Myeloid / Q000139:chemically induced; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced; D008297:Male; D008954:Models, Biological; D009368:Neoplasm Transplantation / Q000379:methods*; D009929:Organ Size; D051381:Rats; D011919:Rats, Inbred Strains; D009396:Wilms Tumor / Q000139:chemically induced
D015127:9,10-Dimethyl-1,2-benzanthracene