An immunohistochemical study of copper, zinc-containing superoxide dismutase detected by a monoclonal antibody gastric mucosa and gastric cancer.
The immunohistochemical localization of copper, zinc-superoxide dismutase (Cu,Zn-SOD) in human gastric mucosa and gastric cancer was studied using a monoclonal antibody. In gastric mucosa, parietal cells, pyloric glandular cells and foci of intestinal metaplasia showed positive staining in the cytoplasm and/or nucleus. The wide distribution of Cu, Zn-SOD in the gastric mucosa suggests cell function may be vulnerable to active oxygen species. In gastric cancer, 34 of 70 cases showed a positive reaction for Cu, Zn-SOD. There was a relationship between the grade of Cu,Zn-SOD immunoreactivity and the histological type of gastric cancer, well-differentiated types of gastric cancer being more frequently positive. The positive cases of poorly-differentiated adenocarcinoma were characterized by a pattern of diffusely infiltrative invasion. These results suggest that some types of gastric cancer are resistant to active oxygen species.
Oka
S
S
Kokura Memorial Hospital, Kitakyusyu, Japan.
Ogino
K
K
Houbara
T
T
Yoshimura
S
S
Okazaki
Y
Y
Takemoto
T
T
Kato
N
N
Iida
Y
Y
Uda
T
T
D000230:Adenocarcinoma / Q000201:enzymology / Q000473:pathology; D002288:Adenocarcinoma, Mucinous / Q000201:enzymology / Q000473:pathology; D000911:Antibodies, Monoclonal; D005609:Free Radicals; D005753:Gastric Mucosa / Q000166:cytology / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008679:Metaplasia; D010100:Oxygen / Q000378:metabolism; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000473:pathology; D013482:Superoxide Dismutase / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D005609:Free Radicals; D013482:Superoxide Dismutase; D010100:Oxygen