title: On the detection of paramagnetic species in the adriamycin-perfused rat heart: a reappraisal.
abstract: Recently, L. Costa et al. reported the direct detection of the superoxide anion and other paramagnetic species in the isolated, adriamycin-perfused rat heart [L. Costa et al. (1988) Biochem. Biophys. Res. Commun. 153, 275-280]. We have reevaluated the results of their study and concluded that the ESR parameters of the spectrum obtained from the adriamycin-perfused heart are consistent with that of the peroxyl radical and not with that of the superoxide anion. In addition, the ESR spectrum of the peroxyl radical is very likely produced as an artifact caused by the grinding of myocardial tissue. This artifact may mask the ESR spectra of the adriamycin-derived semiquinone radical and the iron-sulfur protein components of myocardium.
authors.affiliation: Department of Radiology, Medical College of Wisconsin, Milwaukee 53226.
authors.forename: B
authors.initials: B
authors.lastname: Kalyanaraman
authors.forename: J E
authors.initials: JE
authors.lastname: Baker
mesh_terms: D000818:Animals; D003327:Coronary Disease / Q000378:metabolism; D004317:Doxorubicin / Q000378:metabolism / Q000633:toxicity*; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D006321:Heart / Q000187:drug effects*; D066298:In Vitro Techniques; D007506:Iron-Sulfur Proteins / Q000032:analysis; D009206:Myocardium / Q000032:analysis* / Q000378:metabolism; D010477:Perfusion; D010545:Peroxides / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013481:Superoxides / Q000032:analysis*
chemical_list: D005609:Free Radicals; D007506:Iron-Sulfur Proteins; D010545:Peroxides; D013481:Superoxides; D004317:Doxorubicin