Effects of histamine on alpha adrenergic receptor expression on the lymphocytes of normal and asthmatic subjects.
Low levels of [3H]-dihydroergocryptine (DHE) (alpha adrenergic receptor) binding to peripheral blood mononuclear leukocytes from asthmatic subjects were significantly increased following incubation with histamine. In contrast, [3H]-DHE binding was undetectable both before and after histamine incubation using cells from nonasthmatic subjects. The change in binding showed a biphasic dose dependence on histamine and was not associated with changes in DHA (beta-2 receptor) or yohimbine (alpha-2 receptor) binding. The induction of alpha adrenergic receptors by the ubiquitous inflammatory mediator histamine may help explain the alpha adrenergic hyperresponsiveness seen in asthmatic patients.
Herman
F F
FF
Department of Allergy and Clinical Immunology, Kaiser-Permanente Medical Center, Los Angeles, CA 90027.
Goldberg
B J
BJ
Lad
P M
PM
Neidzin
H
H
Kaplan
M
M
Easton
J G
JG
D001249:Asthma / Q000097:blood*; D004082:Dihydroalprenolol / Q000378:metabolism; D004088:Dihydroergotoxine / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006632:Histamine / Q000494:pharmacology*; D006801:Humans; D009000:Monocytes / Q000378:metabolism*; D011224:Prazosin / Q000378:metabolism; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism*; D012016:Reference Values; D015016:Yohimbine / Q000378:metabolism
D011942:Receptors, Adrenergic, alpha; D004088:Dihydroergotoxine; D015016:Yohimbine; D004082:Dihydroalprenolol; D006632:Histamine; D011224:Prazosin