Cloning and identification of a rabbit sperm membrane protein gene.
Antibodies raised against rabbit sperm membrane protein (rSMP-B) interacted with a rat testis cytosolic protein with an estimated molecular weight of 70 kD. The clone expressing rSMP-B was isolated by epitope selection from a rat testis lambda gt11 vector cDNA library. An Eco R1-cut 2.0 kb cDNA fragment encoding rSMP-B was identified. The cDNA fragment (RSD-1) was isolated, subcloned and restriction mapped. Dot hybridization analysis of mRNA prepared from human, rabbit and rat testes showed that the gene encoding rSMP-B is expressed in the testes of all three mammals.
Wang
L
L
Institute of Basic Medical Sciences, CAMS, Beijing.
Miao
S
S
Yan
Y
Y
Wang
W
W
Liu
Q
Q
Bai
Y
Y
Zhu
H
H
Koide
S
S
Chen
C
C
Bardin
C W
CW
D000818:Animals; D000906:Antibodies; D000954:Antigens, Surface; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D015870:Gene Expression; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D010446:Peptide Fragments / Q000302:isolation & purification; D011817:Rabbits; D051381:Rats; D013737:Testis / Q000032:analysis
D000906:Antibodies; D000954:Antigens, Surface; D004251:DNA Transposable Elements; D008565:Membrane Proteins; D010446:Peptide Fragments; C049242:rSMP-B protein, human