Growth of avian adeno-associated virus in chicken cells transfected with fowl adenovirus serotype 1 DNA.
Using the chloroquine-modified calcium phosphate coprecipitation technique, fowl adenovirus serotype 1 (FAV 1) DNA transfects efficiently chicken cell cultures. Infection of FAV 1 DNA transfected cells with helper dependent avian adeno-associated virus (AAAV) results in the production of AAAV progeny, being detected by an indirect immunofluorescence assay. These findings indicate that FAV 1 DNA introduced into the host cell promotes actively the growth of AAAV.
Bauer
A
A
Institute of Poultry Diseases, Free University of Berlin, F.R.G.
Monreal
G
G
Bauer
H J
HJ
D000257:Adenoviridae Infections / Q000175:diagnosis / Q000188:drug therapy / Q000662:veterinary; D000818:Animals; D000259:Aviadenovirus / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D002645:Chickens; D002738:Chloroquine / Q000494:pharmacology; D004279:DNA, Viral / Q000032:analysis; D005455:Fluorescent Antibody Technique; D011201:Poultry Diseases / Q000175:diagnosis / Q000188:drug therapy; D014162:Transfection; D014779:Virus Replication
D004279:DNA, Viral; D002738:Chloroquine