title: Stretch may enhance the release of endothelium-derived relaxing factor in rabbit aorta.
abstract: The effect of vascular stretch on the release of EDRF was studied by measuring tissue cGMP levels of rabbit. Aortic rings of rabbit were quick-frozen in liquid nitrogen during varying resting tensions, and cGMP contents were determined by radio-immunoassay. The tissue cGMP levels significantly elevated with the increase in resting tension in endothelium-intact rings, but not in endothelium-denuded rings. Deprivation of extracellular calcium abolished the stretch-induced elevation of tissue cGMP levels in endothelium-intact segments. These stretch-induced endothelium dependent tissue cGMP elevations were unaffected by Ca2+ channel blockers, nicardipine and diltiazem. Data suggest that vascular stretch may release EDRF via mechanism dependent on extracellular calcium, but probably not through voltage-dependent calcium channel.
authors.affiliation: First Department of Internal Medicine, University of Tokyo, Faculty of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ohno
authors.forename: M
authors.initials: M
authors.lastname: Ochiai
authors.forename: J
authors.initials: J
authors.lastname: Taguchi
authors.forename: K
authors.initials: K
authors.lastname: Hara
authors.forename: N
authors.initials: N
authors.lastname: Akatsuka
authors.forename: K
authors.initials: K
authors.lastname: Kurokawa
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism*; D004110:Diltiazem / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009529:Nicardipine / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D010812:Physical Stimulation; D011817:Rabbits; D011863:Radioimmunoassay
chemical_list: D015220:Calcium Channels; D009569:Nitric Oxide; D009529:Nicardipine; D004110:Diltiazem; D006152:Cyclic GMP; D002118:Calcium