A refined complement-enhanced neutralization test for detecting antibodies to Junin virus.
A refined, complement-enhanced, plaque-reduction neutralization test was developed for measuring neutralizing antibodies against Junin (Argentine hemorrhagic fever) virus. The assay measured neutralizing antibodies after natural as well as vaccine-induced Junin virus infections. Among vaccinated individuals, titers were 2-4-fold higher than those obtained with conventional assays, without loss of specificity. Enhanced sensitivity was achieved by using a standardized complement source (vs human or animal serum) for virus dilution, incubation of virus-serum mixtures at 36 degrees C for 2 h (vs overnight at 4 degrees C) prior to plaque assay, control of age and density of cell monolayers, and variation in overlay conditions.
Barrera Oro
J G
JG
Salk Institute, Government Services Division, Swiftwater, Pennsylvania.
McKee
K T
KT
Spisso
J
J
Mahlandt
B G
BG
Maiztegui
J I
JI
D000704:Analysis of Variance; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D006477:Arenaviruses, New World / Q000276:immunology*; D006478:Hemorrhagic Fever, American / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D009500:Neutralization Tests / Q000379:methods*; D014709:Vero Cells; D014765:Viral Vaccines / Q000008:administration & dosage
D000914:Antibodies, Viral; D014765:Viral Vaccines