Polyclonal antibodies to mannan from yeast also recognize the carbohydrate structure of gp120 of the AIDS virus: an approach to raise neutralizing antibodies to HIV-1 infection in vitro.
This study initiates a new method of developing an antigen which might be useful in the prevention of HIV-1 infection. Using a mannan preparation from Saccharomyces cerevisiae neutralizing antiserum was raised in rabbits which prevents HIV-1 infection in vitro up to a titre of 1:128. The corresponding antibody preparation neutralized the in vitro infectivity down to a concentration of 5 micrograms/ml. Analytical studies suggest that the antibodies are directed against the mannose residues of the HIV-1 glycoprotein (gp) 120 and its precursor gp 160.
Müller
W E
WE
Institut für Physiologische Chemie, Abteilung Angewandte Molekularbiologie, Universität, Mainz, FRG.
Schröder
H C
HC
Reuter
P
P
Maidhof
A
A
Uhlenbruck
G
G
Winkler
I
I
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000517:prevention & control; D000818:Animals; D000946:Antigens, Fungal; D001665:Binding Sites; D002241:Carbohydrates / Q000276:immunology; D002460:Cell Line; D005260:Female; D015483:HIV Antibodies / Q000096:biosynthesis / Q000276:immunology*; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D066298:In Vitro Techniques; D008351:Mannans / Q000276:immunology*; D009500:Neutralization Tests; D011817:Rabbits; D012441:Saccharomyces cerevisiae / Q000276:immunology
D000946:Antigens, Fungal; D002241:Carbohydrates; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D008351:Mannans