Transcriptional activation of the adenine phosphoribosyltransferase promoter by an upstream butyrate-induced Moloney murine sarcoma virus enhancer-promoter element.
It has been documented that the activity of a specific promoter can be occluded by the presence of another promoter element upstream. We present evidence for a phenomenon contradictory to that predicted by the promoter occlusion theory. Transcription from the hamster aprt (adenine phosphoribosyltransferase) promoter was augmented instead of repressed in transfected mouse L cells when an upstream Moloney murine sarcoma virus enhancer-promoter element was induced with butyrate. Without an adjacent Moloney murine sarcoma virus element, butyrate could not activate the aprt promoter.
Tang
D C
DC
Department of Biology, Indiana University, Bloomington 47405.
Taylor
M W
MW
D000228:Adenine Phosphoribosyltransferase / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D006224:Cricetinae; D004742:Enhancer Elements, Genetic / Q000187:drug effects; D015967:Gene Expression Regulation, Viral; D007739:L Cells / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D008980:Moloney murine sarcoma virus / Q000235:genetics*; D009710:Nucleotide Mapping; D010430:Pentosyltransferases / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000187:drug effects; D015183:Restriction Mapping; D012260:Ribonucleases; D009053:Sarcoma Viruses, Murine / Q000235:genetics*; D015533:Transcriptional Activation; D014162:Transfection
D002087:Butyrates; D020148:Butyric Acid; D010430:Pentosyltransferases; D000228:Adenine Phosphoribosyltransferase; D012260:Ribonucleases