title: Mycoplasma pneumoniae DNA gyrase genes.
abstract: We have identified a clone from a lambda EMBL3 library containing a 19kb insert of Mycoplasma pneumoniae DNA which includes the genes that encode both subunits of DNA gyrase. The gyrB gene and the 5' end of the gyrA gene have been subcloned into M13. The gyrB gene is 1953bp in length and overlaps the gyrA gene by a single base. The nucleotide sequence of these subclones has significant homology to previously reported gyrase genes. In terms of the size of the gyrB gene and its proximity to the gyrA gene, M. pneumoniae is more similar to Bacillus subtilis than to Escherichia coli.
authors.affiliation: Department of Pediatrics, University of North Carolina School of Medicine, Chapel Hill 27599.
authors.forename: S D
authors.initials: SD
authors.lastname: Colman
authors.forename: P C
authors.initials: PC
authors.lastname: Hu
authors.forename: K F
authors.initials: KF
authors.lastname: Bott
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004250:DNA Topoisomerases, Type II / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009177:Mycoplasma pneumoniae / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004250:DNA Topoisomerases, Type II