Transcription initiated by RNA polymerase II and purified transcription factors from liver. A complex set of promoter sequences governs formation of the initial complex.
Using a transcription system derived from rat liver, we have investigated the function of the TATA-box and cap site/initiator regions in assembly of stable intermediates on the pathway leading to formation of a functional RNA polymerase II preinitiation complex. Our findings argue that neither the TATA-box region nor the cap site/initiator region functions independently in the binding of transcription factors during formation of these intermediates, but, rather, the two sequence elements function cooperatively to direct assembly of a stable preinitiation complex at the promoter.
Conaway
J W
JW
Department of Chemistry, University of Texas, Austin 78712-1096.
Travis
E
E
Conaway
R C
RC
D000818:Animals; D001483:Base Sequence; D008297:Male; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012319:RNA Polymerase II / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013698:Templates, Genetic; D014157:Transcription Factors / Q000302:isolation & purification / Q000378:metabolism*; D014158:Transcription, Genetic
D014157:Transcription Factors; D012319:RNA Polymerase II