The average number of molecules of Epstein-Barr nuclear antigen 1 per cell does not correlate with the average number of Epstein-Barr virus (EBV) DNA molecules per cell among different clones of EBV-immortalized cells.
Epstein-Barr nuclear antigen 1 (EBNA-1) is the only viral protein required to support latent replication of Epstein-Barr virus (EBV). To assess the likelihood that EBNA-1 regulates the amount of EBV DNA in a cell, we measured the average numbers of EBNA-1 molecules and EBV DNA molecules per cell in different clones of cells. The amount of EBNA-1 protein present in recently established lymphoblastoid cell lines was measured with affinity-purified anti-EBNA-1 antibodies, and viral DNA was measured by nucleic acid hybridization. The average levels of EBNA-1 protein varied little between these cell lines, whereas the average amount of viral DNA present varied substantially; consequently, these numbers were not correlated. There is no apparent relationship between amounts of EBNA-1 and viral DNA.
Sternås
L
L
McArdle Laboratory for Cancer Research, University of Wisconsin-Madison 53706.
Middleton
T
T
Sugden
B
B
D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D002472:Cell Transformation, Viral; D002999:Clone Cells; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D019309:Epstein-Barr Virus Nuclear Antigens; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007106:Immune Sera
D000914:Antibodies, Viral; D000956:Antigens, Viral; D004279:DNA, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D007106:Immune Sera