title: Nucleotide sequence of the COR region: a cluster of six genes in the yeast Saccharomyces cerevisiae.
abstract: We have determined the nucleotide (nt) sequence of the 7.5-kb COR segment that encompasses a cluster of six genes (CYC1, UTR1, UTR3, OSM1, tRNA(Gly) and RAD7) located on chromosome X of the yeast Saccharomyces cerevisiae. This sequence revealed five open reading frames and a tRNA gene which correspond in position, size and orientation to the transcripts previously identified by Barry et al. [Mol. Cell. Biol. 7 (1987) 632-638]. The extensively studied CYC1 gene encodes iso-1-cytochrome c; the UTR1 and UTR3 genes encode dispensible proteins whose functions are unknown; the OSM1 gene encodes a protein required for growth on hypertonic media; the tRNA(Gly) gene encodes a glycine tRNA; and the RAD7 gene encodes a protein required for repair of UV-induced damage. The OSM1 protein contains a signal sequence for secretion and a region similar to GTP-binding domains. The RAD7 protein displays 5'-untranslated elements similar to those of the stress-inducible gene UB14. The nt sequence upstream from the tRNA(Gly) gene contains a diverged copy of the sigma repeated element. This cluster of COR genes appears to have an ancestral relationship with the cluster of ARC genes on chromosome V.
authors.affiliation: Department of Biochemistry, University of Rochester School of Medicine and Dentistry, NY 14642.
authors.forename: L
authors.initials: L
authors.lastname: Melnick
authors.forename: F
authors.initials: F
authors.lastname: Sherman
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015825:Chromosomes, Fungal; D003574:Cytochrome c Group / Q000235:genetics; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000235:genetics*; D019204:GTP-Binding Proteins / Q000235:genetics; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D005810:Multigene Family; D010641:Phenotype; D012353:RNA, Transfer, Gly / Q000235:genetics; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D012689:Sequence Homology, Nucleic Acid; D012808:Sigma Factor / Q000235:genetics; D013385:Succinate Dehydrogenase
chemical_list: D003574:Cytochrome c Group; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C063916:OSM1 protein, S cerevisiae; C063915:RAD7 protein, S cerevisiae; D012353:RNA, Transfer, Gly; D029701:Saccharomyces cerevisiae Proteins; D012808:Sigma Factor; D013385:Succinate Dehydrogenase; D019204:GTP-Binding Proteins