Nucleotide sequence of Streptomyces fradiae transposable element Tn4556: a class-II transposon related to Tn3.
The first transposable element to be isolated from Streptomyces fradiae, Tn4556, was completely sequenced; the total of 6625 bp have an overall G + C composition of 68%. Computer-aided analysis of this sequence reveals the location of nine open reading frames (ORFs). Several of these ORFs, numbers 1, 2, and 7, contain ribosome-binding sites (RBS) near their putative translation-initiation sites, which share identity with the consensus RBS sequences of Escherichia coli and Bacillus subtilis. ORF1 potentially encodes an 892-amino acid (aa) protein and this deduced aa sequence shares 61% identity with that of the transposase encoded by the tnpA gene of Tn3. Three other ORFs, 2, 3 and 5, potentially encode proteins which are similar in size to the resolvase protein encoded by the Tn3 gene tnpR; however, none of the protein products deduced from these ORF share extensive aa sequence identity with other resolvase proteins.
Siemieniak
D R
DR
Molecular Biology Research, Upjohn Company, Kalamazoo, MI 49007.
Slightom
J L
JL
Chung
S T
ST
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D005075:Biological Evolution; D003001:Cloning, Molecular; D003062:Codon; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D012045:Regulatory Sequences, Nucleic Acid; D013302:Streptomyces / Q000235:genetics*
D001426:Bacterial Proteins; D003062:Codon; D004251:DNA Transposable Elements; D004269:DNA, Bacterial