Phosphoinositide signalling system in human erythrocyte and its role in cell pathology.
Human erythrocytes contain 217.2 +/- 70.5 pmole of inositol 1,4,5-trisphosphate per ml of packed cells (n = 14). The increased generation of Ins 1,4,5P3, was induced by 1 microM A 23187, 20 microM Pb2+, and by AlF4-. Ins 1,4,5P3, Pb2+ and AlF4- evoke shape changes, disorganisation of spectrin network and vesiculation. In erythrocytes of patients with hereditary spherocytosis the level of Ins 1,4,5P3 was increased to 556.7 +/- 374 pmole per ml of packed cells. We suggest that activation of the phosphoinositide signalling system may represent a common denominator for many divergent stimuli and defects which affect shape changes of erythrocytes.
Strunecká
A
A
Department of Physiology and Developmental Biology, Faculty of Sciences, Charles University, Prague, Czechoslovakia.
Kmonícková
E
E
Krpejsová
L
L
el Desouki
N I
NI
Hrusová
H
H
Palecek
J
J
D000001:Calcimycin / Q000494:pharmacology; D004907:Erythrocyte Deformability / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols / Q000502:physiology*; D015290:Second Messenger Systems / Q000187:drug effects
D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols; D000001:Calcimycin