title: Topostin, a novel inhibitor of mammalian DNA topoisomerase I from Flexibacter topostinus sp. nov. I. Taxonomy, and fermentation of producing strain.
abstract: We found a new inhibitor of mammalian DNA topoisomerase I, named topostin, from a bacterial culture. The bacteria was identified as Flexibacter topostinus sp. nov., B-572. Morphological and physiological characteristics, and utilization of sugars were examined. Comparison of the strain with known species of the genus Flexibacter was made and indicates that the strain is a new species of the genus Flexibacter. The bacteria produced the inhibitor in parallel with their growth up to 72 hours.
authors.affiliation: Bioscience Research Laboratories, Yamanouchi Pharmaceutical Co., Ltd., Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: S
authors.initials: S
authors.lastname: Miyazaki
authors.forename: K
authors.initials: K
authors.lastname: Nagai
authors.forename: S
authors.initials: S
authors.lastname: Watanabe
authors.forename: T
authors.initials: T
authors.lastname: Saito
authors.forename: K
authors.initials: K
authors.lastname: Ishii
authors.forename: M
authors.initials: M
authors.lastname: Hanada
authors.forename: T
authors.initials: T
authors.lastname: Sekine
authors.forename: Y
authors.initials: Y
authors.lastname: Ikegami
mesh_terms: D003470:Culture Media / Q000032:analysis; D003589:Cytophaga / Q000378:metabolism* / Q000648:ultrastructure; D004264:DNA Topoisomerases, Type I / Q000302:isolation & purification / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000302:isolation & purification*; D005285:Fermentation; D009930:Organic Chemicals; D059004:Topoisomerase I Inhibitors
chemical_list: D003470:Culture Media; D004791:Enzyme Inhibitors; D009930:Organic Chemicals; D059004:Topoisomerase I Inhibitors; C063442:topostin A1; C063443:topostin A2; C063444:topostin B; D004264:DNA Topoisomerases, Type I