Investigation of ovarian neoplasia of low malignant potential for human papillomavirus.
Recent in situ hybridization studies have suggested the presence of human papillomavirus type 6 (HPV-6) DNA in ovarian cancer cells. An association between HPV and ovarian neoplasia of low malignant potential (LMP) has not been previously identified. Paraffin-embedded tissue blocks from 24 patients with LMP ovarian tumors were screened for human papillomavirus DNA. The patients ranged in age from 18 to 73 years. Corresponding microscopic slides from each tissue block were reviewed to confirm the histopathologic diagnosis. For identification of HPV genome, deparaffinized sections were subjected to the polymerase chain reaction to achieve amplification of DNAs of HPV types 6, 11, 16, and 18. For each HPV type, a 120-base-pair region of the E6 gene was targeted for amplification. Human papillomaviral DNA was not detected in the tissue specimens subjected to polymerase chain reaction. These results suggest that HPV types 6, 11, 16, and 18 are not likely to play a role in LMP ovarian tumors. These results do not totally exclude possible contributions of other HPV types.
McLellan
R
R
Department of Gynecology and Obstetrics, Johns Hopkins Hospital, Baltimore, Maryland 21205.
Buscema
J
J
Guerrero
E
E
Shah
K V
KV
Woodruff
J D
JD
Currie
J L
JL
D000293:Adolescent; D000328:Adult; D000368:Aged; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005784:Gene Amplification; D006801:Humans; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000382:microbiology*; D027383:Papillomaviridae / Q000302:isolation & purification*
D004279:DNA, Viral