title: Effect of dietary oils on the production of n-3 and n-6 metabolites of leukocyte 5-lipoxygenase in five rat strains.
abstract: We examined the influence of various dietary oils, including linseed and fish oil on the relative rates of leukotriene B4 (LTB4) and LTB5 production by rat peritoneal exudate cells in five rat strains. While there was an association between the membrane phospholipid levels of the fatty acid precursors (arachidonic acid (AA) and eicosapentaenoic acid (EPA)) and the rate of synthesis of their respective 5-lipoxygenase products (LTB4 and LTB5), the rate of LTB4 synthesis was a combined function of both AA and EPA levels. We observed a strong linear relationship (correlation coefficient = 0.99) between the ratio of EPA/AA in the cell membrane phospholipids and the ratio of LTB5/LTB4 produced by these cells in vitro; this association was independent of genetic (strain) variability and was independent of the source of EPA (dietary EPA or EPA endogenously synthesized from dietary alpha-linolenic acid).
authors.affiliation: Morialta Trust Bone and Joint Research Laboratory, Royal Adelaide Hospital, Australia.
authors.forename: L G
authors.initials: LG
authors.lastname: Cleland
authors.forename: M J
authors.initials: MJ
authors.lastname: James
authors.forename: R A
authors.initials: RA
authors.lastname: Gibson
authors.forename: J S
authors.initials: JS
authors.lastname: Hawkes
authors.forename: W H
authors.initials: WH
authors.lastname: Betts
mesh_terms: D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism*; D001091:Arachidonate Lipoxygenases / Q000378:metabolism*; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D004041:Dietary Fats / Q000378:metabolism*; D015118:Eicosapentaenoic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D005395:Fish Oils / Q000378:metabolism; D066298:In Vitro Techniques; D007975:Leukotriene B4 / Q000378:metabolism* / Q000031:analogs & derivatives; D008043:Linseed Oil / Q000378:metabolism; D009821:Oils / Q000378:metabolism*; D000069463:Olive Oil; D010529:Peritoneal Cavity / Q000166:cytology; D010743:Phospholipids / Q000378:metabolism; D010938:Plant Oils / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D000074242:Sunflower Oil
chemical_list: D001095:Arachidonic Acids; D004041:Dietary Fats; D005395:Fish Oils; D009821:Oils; D000069463:Olive Oil; D010743:Phospholipids; D010938:Plant Oils; D000074242:Sunflower Oil; D007975:Leukotriene B4; D016718:Arachidonic Acid; D008043:Linseed Oil; C040212:leukotriene B5; D015118:Eicosapentaenoic Acid; D001091:Arachidonate Lipoxygenases; D001094:Arachidonate 5-Lipoxygenase