Purification of genomic sized herpesvirus DNA using pulse-field electrophoresis.
A new method for the separation of genomic sized herpesvirus DNA was developed. This method utilizes the improved separation of extremely high molecular weight DNA molecules by pulse-field electrophoresis. This method has allowed us to separate and purify herpesvirus DNA (greater than 100 kbp) free of cellular DNA, directly from infected cells. In the case of cell associated herpesviruses (such as Marek's disease herpesvirus), this method is more efficient than present isolation procedures. The DNA obtained is able to be restriction enzyme digested and used in cloning experiments.
Isfort
R J
RJ
Genetic Toxicology Section, Procter and Gamble Company, Cincinnati, OH 45239.
Robinson
D
D
Kung
H J
HJ
D004279:DNA, Viral / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005814:Genes, Viral; D006564:Herpesviridae / Q000235:genetics*; D008381:Herpesvirus 2, Gallid / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000235:genetics; D018139:Simplexvirus / Q000235:genetics
D004279:DNA, Viral