Use of positive ion fast atom bombardment mass spectrometry for rapid identification of a bile alcohol glucuronide isolated from cerebrotendinous xanthomatosis patients.
The identification of a major biliary and plasma bile alcohol glucuronide, 5 beta-cholestane-3 alpha, 7 alpha, 12 alpha, 25-tetrol-3-0-beta-D-glucuronide, present in cerebrotendinous xanthomatosis (CTX) patients, was investigated by positive ion fast atom bombardment mass spectrometry (FAB-MS). The spectrum was characterized by abundant ions formed by attachment of a proton, [M + H]+, or of alkali ions, [M + Na]+ and [M + 39K]+, to the glucuronide salt. These ions allowed an unambiguous deduction of the molecular weight of the sample. It is suggested that FAB-MS could be used in the rapid diagnosis of CTX.
Dayal
B
B
Department of Medicine, UMDNJ-New Jersey Medical School, Newark.
Salen
G
G
Tint
G S
GS
Shefer
S
S
Benz
S W
SW
D001649:Bile Duct Diseases / Q000175:diagnosis*; D055598:Chemical Phenomena; D002621:Chemistry; D002777:Cholestanols / Q000032:analysis* / Q000097:blood; D004583:Electrons; D005965:Glucuronates / Q000032:analysis* / Q000097:blood; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D012680:Sensitivity and Specificity; D014973:Xanthomatosis / Q000175:diagnosis*
D002777:Cholestanols; D005965:Glucuronates