A species-specific sperm-activating peptide from the egg jelly of the sea urchin Diadema setosum.
1. A species-specific sperm-activating peptide was isolated from the egg jelly of the sea urchin Diadema setosum and the amino acid sequence was determined as follows: (formula; see text). 2. The peptide caused significant increases of respiration rates and cyclic nucleotide concentrations in D. setosum spermatozoa as low as 10(-9) M. 3. The addition of the peptide to D. setosum spermatozoa resulted in the appearance of a newly stained protein (mol. wt 128,000) on sodium dodecyl sulfate-polyacrylamide gels.
Yoshino
K
K
Noto Marine Laboratory, Kanazawa University, Ishikawa, Japan.
Kurita
M
M
Yamaguchi
M
M
Nomura
K
K
Takao
T
T
Shimonishi
Y
Y
Suzuki
N
N
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000242:Cyclic AMP / Q000032:analysis; D006152:Cyclic GMP / Q000032:analysis; D008297:Male; D008969:Molecular Sequence Data; D010063:Ovum / Q000032:analysis; D010101:Oxygen Consumption; D010456:Peptides, Cyclic / Q000302:isolation & purification; D012617:Sea Urchins / Q000032:analysis*; D013045:Species Specificity; D013094:Spermatozoa / Q000502:physiology
D000596:Amino Acids; D010456:Peptides, Cyclic; C063869:sperm-activating peptide IV, Diadema setosum; D000242:Cyclic AMP; D006152:Cyclic GMP