Immunohistochemical demonstration of nuclear S1 proteins in various cells.
S1 proteins are present in the nuclear structures sensitive to DNases and RNase. To examine localization of these proteins, an antibody was raised in a rabbit. Indirect immunofluorescence staining revealed that S1 proteins located in the extranucleolar nuclear regions of quiescent myocardial and cerebellar cells as well as actively duplicating mouse 3T3 fibroblasts. They located in euchromatin regions of thymus lymphocytes, with a characteristic aster-like immunofluorescence pattern, and on the border of condensed chromatin areas by deposition of immunogold particles in ultrathin sections of thymus. Thus, S1 proteins may be in a nuclear function assigned to the border of heterochromatin areas, and other than synthesis of DNA or of ribosomal RNA. Possible involvement of S1 proteins in the extranucleolar RNA synthesis is discussed.
Takahashi
K P
KP
Department of Anatomy, Osaka City University Medical School, Japan.
Higashi
Y
Y
Uchimoto
T
T
Hasuma
T
T
Yamamoto
N
N
Morisawa
S
S
Inoue
A
A
D000818:Animals; D000906:Antibodies / Q000276:immunology; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D007150:Immunohistochemistry; D051379:Mice; D009687:Nuclear Proteins / Q000276:immunology*; D011817:Rabbits
D000906:Antibodies; D009687:Nuclear Proteins