Influence of adenosine cyclic nucleotide analogs on growth of human colon tumor cell lines.
The influence of three analogs of cyclic adenosine monophosphate (cAMP) and theophylline on growth of colon tumor cell lines HT 29, LIM 1215 and COLO 206F was assessed by serial estimates of cell number. Administration of theophylline or analog of cAMP 8-bromo cAMP (8-br-cAMP) to actively replicating cultures resulted in a decrease in cell number of each cell line. In contrast analogs of cAMP, dibutyryl cAMP (db-cAMP) and chlorophenylthio cAMP (cp-cAMP) caused an increase in cell number of each cell line. This variation between analogs makes it difficult to draw conclusions regarding the influence of cAMP on cell growth when analogs are used to mimic the biological role of cAMP.
Saba
M V
MV
Department of Anatomy, Monash University, Clayton, Victoria, Australia.
Barkla
D H
DH
Tutton
P J
PJ
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D003994:Bucladesine / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000503:physiopathology; D000242:Cyclic AMP / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D013806:Theophylline / Q000494:pharmacology; D013873:Thionucleotides / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D013873:Thionucleotides; D015124:8-Bromo Cyclic Adenosine Monophosphate; C015929:8-((4-chlorophenyl)thio)cyclic-3',5'-AMP; D003994:Bucladesine; D013806:Theophylline; D000242:Cyclic AMP