In vitro effect of actinomycin D on human neutrophil function.
The effect of actinomycin D (ACT-D) on human neutrophil chemotaxis, chemiluminescence (CL), superoxide (O2-) production, phagocytic uptake, and intracellular bacterial killing has been examined. The viability of the ACT-D-treated neutrophils was 98% even at a concentration of 10 micrograms/ml for 4 hr. Using fMLP as the chemotactic factor, depressed chemotaxis was demonstrated following ACT-D (1-10 micrograms/ml) pretreatment of neutrophils as compared with the non-treated controls. Similar ACT-D pretreatment produced the depressed responses in phorbol myristate acetate-induced CL and superoxide production by neutrophils. Moreover, using heat-inactivated human serum as an opsonin for Salmonella enteritidis (NCTC 6676), there was a significant difference in intracellular killing (P less than 0.01) but no difference in phagocytic uptake between ACT-D-treated and non-treated neutrophils. These studies indicate that ACT-D profoundly impairs both intracellular bacterial killing by human neutrophil through an effect on respiratory burst activity and directed cell migration of human neutrophils.
Chang
F Y
FY
Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, R.O.C.
Shaio
M F
MF
D000906:Antibodies / Q000276:immunology; D002470:Cell Survival; D002634:Chemotaxis, Leukocyte; D003609:Dactinomycin / Q000494:pharmacology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D066298:In Vitro Techniques; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D009895:Opsonin Proteins / Q000276:immunology; D010587:Phagocytosis; D012477:Salmonella enteritidis / Q000276:immunology; D013481:Superoxides / Q000378:metabolism
D000906:Antibodies; D007074:Immunoglobulin G; D009895:Opsonin Proteins; D013481:Superoxides; D003609:Dactinomycin