Induction of Mx-related protein in cat peripheral blood mononuclear cells after administration of recombinant human interferon hybrid.
A 78 kDa protein was induced in cat peripheral blood mononuclear cells after in vivo administration of recombinant human interferon-alpha hybrid (rHuIFN-alpha B/D). This protein was antigenically related to the IFN-induced human (78 kDa) and mouse Mx proteins. Quantitative immunoblot analysis indicated that the induction of the cat Mx protein was dose-dependent. There was a dissociation in time between plasma levels of IFN which were transient, and levels of cat Mx protein which remained elevated at least five days after dosing. Our results provide evidence that a human IFN-alpha hybrid may be active in cats. They also indicate that the Mx protein is a sensitive, quantitative, and stable marker to follow IFN activity in vivo in cats.
Horisberger
M A
MA
Ciba-Geigy Ltd., Biotechnology, Pharmaceutical Research, Basel, Switzerland.
Schrenk
R
R
Staiger
S
S
Leyvraz
A R
AR
Martinod
S
S
D000818:Animals; D015415:Biomarkers; D002415:Cats; D019204:GTP-Binding Proteins; D006801:Humans; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology; D064547:Myxovirus Resistance Proteins; D014176:Protein Biosynthesis; D011994:Recombinant Proteins; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects
D015415:Biomarkers; D007370:Interferon Type I; C579070:Mx1 protein, mouse; D064547:Myxovirus Resistance Proteins; D011994:Recombinant Proteins; D019204:GTP-Binding Proteins