Modulation of fatty acid-binding capacity of heart fatty acid-binding protein by oxygen-derived free radicals.
In this study, we examined the effects of exposure of heart fatty acid-binding protein (h-FABP) to chemically generated O2- or OH. with respect to its oleate binding and to its electrophoretic properties. Purified rat h-FABP at 40 microM scavenged as much as 30% O2- and 85% OH.. On the other hand, when 2 nmol (4 microM) FABP were exposed to free radicals, the maximum oleate binding capacity as measured by Scatchard analysis was reduced only by 14% and 27% for O2- and OH., respectively. The electrophoretic pattern of free radical-exposed FABP was not markedly different when examined either by the non-denaturing or by denaturing PAGE, suggesting the absence of any degradation or aggregation of FABP by O2- or OH.. It is hypothesized that O2- or OH. in physiological concentration may not alter the function of FABP markedly in the ischemic-reperfused myocardium.
Jones
R M
RM
Department of Surgery, University of Connecticut School of Medicine, Farmington 06032.
Prasad
M R
MR
Das
D K
DK
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D003574:Cytochrome c Group / Q000378:metabolism; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000378:metabolism*; D005609:Free Radicals; D006321:Heart / Q000187:drug effects; D006878:Hydroxides; D017665:Hydroxyl Radical; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000494:pharmacology; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism*; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D014969:Xanthine Oxidase / Q000378:metabolism
D002352:Carrier Proteins; D003574:Cytochrome c Group; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D005609:Free Radicals; D006878:Hydroxides; D007042:Hypoxanthines; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D019271:Hypoxanthine; D017665:Hydroxyl Radical; D014969:Xanthine Oxidase; D010100:Oxygen