title: The respiration of cells and mitochondria of porin deficient yeast mutants is coupled.
abstract: Several mutants of yeast lacking the porin gene have been found stable and viable on glucose or glycerol media. Ethanol-supported respiration of porin-free mutant and wild cells appeared equally coupled in vivo being similarly depressed by inhibitors of ADP/ATP translocase or of ATP synthase and stimulated by the uncoupler FCCP. The absence of porin in isolated mutant mitochondria hardly impaired the electron flux but increased the requirement for Mg2+ (or Ca2+) and for ADP and carboxyatractylate concentrations necessary to drive effectively state 3 - state 4 and state 4 - state 3 transitions, respectively. The existence of another porin species, possibly controlled by bivalent cations, is postulated.
authors.affiliation: Laboratoire de Physiologie Moléculaire et Cellulaire, IBCN-CNRS, Bordeaux, France.
authors.forename: J
authors.initials: J
authors.lastname: Michejda
authors.forename: X J
authors.initials: XJ
authors.lastname: Guo
authors.forename: G J
authors.initials: GJ
authors.lastname: Lauquin
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D004734:Energy Metabolism; D000431:Ethanol / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D007425:Intracellular Membranes / Q000502:physiology; D008928:Mitochondria / Q000502:physiology*; D009154:Mutation; D009840:Oligomycins / Q000494:pharmacology; D010101:Oxygen Consumption; D018272:Porins; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D005656:Fungal Proteins; D009840:Oligomycins; D018272:Porins; D000431:Ethanol; D000244:Adenosine Diphosphate