Dibutyryl cAMP-induced increases in triacylglycerol lipase activity in developing L8 myotube cultures.
Triacylglycerol (TG) lipase activity, with an alkaline pH optimum, has been identified in the cellular fraction of L8 myotube cultures. This TG lipase activity was stimulated by serum and inhibited by NaCl and protamine sulfate. These characteristics have been classically described for lipoprotein lipase. It was possible to increase the activity of this TG lipase three- to five-fold by incubating the cells with dibutyryl cAMP. Maximal enzyme activity was observed 16 h following the addition of 10-100 microM dibutyryl cAMP to the cultured cells. Enzyme activity returned to control levels 24 h after removal of the nucleotide from the culture medium. Serum-sensitive alkaline TG lipase activity was also identified in five other myotube preparations of cultured muscle cells. The highest levels of activity were found in rat skeletal muscle primary, H9, and L6 cell types. The finding that dibutyryl cAMP is an effective inducer of alkaline TG lipase activity provides us with a valuable model to investigate mechanisms regulating synthesis, compartmentalization, and transport of lipoprotein lipase in muscle.
Palmer
W K
WK
Department of Physical Education, University of Illinois, Chicago 60680.
Oscai
L B
LB
Bechtel
P J
PJ
Fisher
G A
GA
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002478:Cells, Cultured; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000378:metabolism*; D024510:Muscle Development; D009132:Muscles / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure; D011479:Protamines / Q000494:pharmacology; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology; D013997:Time Factors
D003470:Culture Media; D011479:Protamines; D012965:Sodium Chloride; D003994:Bucladesine; D008049:Lipase