Integration host factor increases the transpositional immunity conferred by gamma delta ends.
The ends of the bacterial transposon gamma delta contain adjacent binding sites for gamma delta transposase and integration host factor (IHF). IHF+ and IHF- strains were used in conjunction with gamma delta transposon ends containing or lacking the site for IHF binding to determine the role that IHF plays in various gamma delta-mediated transposition events. IHF was not essential for the transposition of gamma delta and seemed to decrease its frequency of transposition about threefold. IHF played no role in determining the distribution of gamma delta inserts into a target replicon, nor did it significantly alter the frequency of simple transpositions. The only clear role discerned for IHF and the terminal IHF-binding sites was in transposition immunity. IHF stimulated the immunity of those plasmids that contain an end of gamma delta, provided the end included the terminal IHF-binding site. For both ends, the degree of stimulation of immunity was similar to the stimulation of binding of transposase by IHF.
Wiater
L A
LA
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510.
Grindley
N D
ND
D001426:Bacterial Proteins / Q000378:metabolism*; D001482:Base Composition; D001483:Base Sequence; D001665:Binding Sites; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005838:Genotype; D034982:Integration Host Factors; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000378:metabolism*; D010957:Plasmids; D015183:Restriction Mapping; D019895:Transposases
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D034982:Integration Host Factors; D009713:Nucleotidyltransferases; D019895:Transposases