Characterization and regulation of phosphatidylglycerolphosphate phosphatase in Saccharomyces cerevisiae.
Phosphatidylglycerophosphatase (EC 3.1.3.27) activity was characterized in mitochondrial extracts from Saccharomyces cerevisiae. The enzyme has a pH optimum of 5.5. Maximum activity occurs in the presence of Triton X-100 (5 mM) and cobalt or magnesium ions (5 mM). The apparent Km for PGP is 14.6 microM. The temperature optimum is between 50 degrees C and 60 degrees C. The enzyme is labile above 50 degrees C. The presence of inositol in growth media results in a slight but reproducible increase in PGPase activity in mitochondrial extracts from glucose-grown cells but not glycerol-grown cells. The inositol effect is not seen in crude cell extracts. Carbon source does not affect PGPase activity in mitochondrial extracts or in crude cell extracts.
Kelly
B L
BL
Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor 48109-0606.
Greenberg
M L
ML
D003035:Cobalt / Q000494:pharmacology; D004795:Enzyme Stability; D005947:Glucose / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007294:Inositol / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D008928:Mitochondria / Q000201:enzymology; D017830:Octoxynol; D010743:Phospholipids / Q000378:metabolism / Q000494:pharmacology; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000648:ultrastructure; D013379:Substrate Specificity; D013696:Temperature
D010743:Phospholipids; D003035:Cobalt; D011092:Polyethylene Glycols; D007294:Inositol; D017830:Octoxynol; D010744:Phosphoric Monoester Hydrolases; C026991:phosphatidylglycerophosphatase; D008274:Magnesium; D005947:Glucose; D005990:Glycerol