Cellular LTB4 production differs in allergic sheep with and without late airway responses.
We tested the hypothesis that allergic sheep that develop both early and late airway responses to inhaled Ascaris suum antigen (late responders) have an increased capacity to generate leukotrienes (LTs) compared with allergic sheep that show only early responses to inhaled antigen (acute responders). To test this hypothesis, we measured LTB4 production, in vitro, by granulocytes isolated from peripheral blood and by macrophages isolated from bronchoalveolar lavage (BAL) from both groups of sheep greater than or equal to 2 wk after the animal's last antigen challenge; LTB4 production by granulocytes isolated from BAL from both groups of sheep 6 and 48 h after local airway challenge with A. suum antigen was also measured. LTB4 production was induced by incubating cells (i.e., either granulocytes or macrophages) with calcium ionophore (A23187, 2 microM) and arachidonic acid (30 microM). LTB4 production was quantitated by high-performance liquid chromatography and verified by radioimmunoassay (RIA). On stimulation peripheral blood granulocytes from late responders (n = 7) produced (means +/- SD/10(6) cells) 13.3 +/- 5.2 ng LTB4 compared with 5.3 +/- 1.5 ng LTB4 (P less than 0.05) for acute responders (n = 7). This increased LTB4 production did not result from variations in granulocyte differential or cyclooxygenase activity (as indicated by RIA measurements of prostaglandin E2 production).(ABSTRACT TRUNCATED AT 250 WORDS)
Chapman
G A
GA
Division of Pulmonary Disease, Mount Sinai Medical Center, Miami Beach, Florida 33140.
Signoretti
F
F
Lauredo
I T
IT
Torrealba
P
P
Sielczak
M W
MW
Ahmed
A
A
Abraham
W M
WM
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism / Q000494:pharmacology; D001200:Ascaris / Q000276:immunology; D000001:Calcimycin / Q000494:pharmacology; D002448:Cell Adhesion; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006967:Hypersensitivity; D066298:In Vitro Techniques; D007975:Leukotriene B4 / Q000096:biosynthesis* / Q000302:isolation & purification; D008168:Lung / Q000502:physiology*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D012016:Reference Values; D012756:Sheep; D007507:Therapeutic Irrigation
D000947:Antigens, Helminth; D001095:Arachidonic Acids; D007975:Leukotriene B4; D016718:Arachidonic Acid; D000001:Calcimycin