A method for determining the relative effect of ligands on A-T and G-C base pairs in DNA: applications to metal ions, protons and two amino acids.
A new method is described for the study of specific interactions of low-molecular ligands with the base pairs of DNA. This method is based on the comparative analysis of melting temperature changes in DNAs of different GC-content in the presence of low molecular weight ligands. In this paper, the method is applied to Mn2+, Ni2+, Co2+ ions, deprotonation, amino acids, beta-alanine and gamma-aminobutyric acid (gamma-ABA). Differences in Tm are affected not only by the changes of relative stability of AT- and GC-pairs, but also by other factors. A theoretical analysis of the sequence specificity of low-molecular ligands on the base pairs in DNA molecules characterized by a high degree of sequence heterogeneity is also presented.
Haroutiunian
S G
SG
Department of Molecular Physics and Biophysics, Yerevan State University, USSR.
Dalian
E B
EB
Aslanian
V M
VM
Lando DYu
Akhrem
A A
AA
D000225:Adenine; D000613:Aminobutyrates; D001482:Base Composition; D002151:Calorimetry; D003596:Cytosine; D004247:DNA / Q000737:chemistry*; D006147:Guanine; D008024:Ligands; D008670:Metals; D009691:Nucleic Acid Denaturation; D011522:Protons; D013941:Thymine; D015091:beta-Alanine
D000613:Aminobutyrates; D008024:Ligands; D008670:Metals; D011522:Protons; D015091:beta-Alanine; D006147:Guanine; C012223:alpha-aminobutyric acid; D003596:Cytosine; D004247:DNA; D000225:Adenine; D013941:Thymine