Uranyl photoprobing of conformational changes in DNA induced by drug binding.
The effect on DNA conformation upon binding of the bis-intercalator, N,N'-diacridinyl spermidine is studied by uranyl mediated DNA photocleavage and gel retardation. It is shown that a characteristic A-tract correlated 10 base pair modulation of the uranyl photocleavage of bent (kinetoplast) DNA is suppressed upon binding of the diacridine. Likewise, the anomalous slow gel electrophoretic migration of bent-DNA is abolished by diacridine binding. At higher diacridine/DNA ratios a DNA conformation in which G-residues become hypersensitive to uranyl photocleavage is induced.
Nielsen
P E
PE
Department of Biochemistry B, Panum Institute, University of Copenhagen, Denmark.
Møllegaard
N E
NE
Jeppesen
C
C
D004247:DNA / Q000032:analysis / Q000187:drug effects* / Q000378:metabolism; D015342:DNA Probes / Q000494:pharmacology*; D004270:DNA, Circular / Q000032:analysis / Q000187:drug effects / Q000378:metabolism; D018105:DNA, Kinetoplast; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D010777:Photochemistry
D015342:DNA Probes; D004270:DNA, Circular; D018105:DNA, Kinetoplast; D004247:DNA