Adrenalectomy prevents the stress-induced decrease in in vivo [3H]Ro15-1788 binding to GABAA benzodiazepine receptors in the mouse.
The effect of a single or repeated swim stress on in vivo benzodiazepine receptor binding to various brain regions in adrenalectomized and sham-operated (control) mice was assessed using the benzodiazepine receptor antagonist, [3H]Ro15-1788. In sham-operated mice the binding of [3H]Ro15-1788 to benzodiazepine receptors was reduced in the hippocampus and hypothalamus (single or repeated stress) and cerebral cortex (repeated swim stress) compared to non-stressed mice. In contrast, no alterations in [3H]Ro15-1788 binding were observed in any brain region in adrenalectomized mice after either single or repeated swim stress. These data suggest that an intact hypothalamic-pituitary-adrenal axis is required for the stress-induced decrease in benzodiazepine receptor occupancy measured using the in vivo binding method.
Weizman
A
A
Section on Molecular Pharmacology, National Institute on Mental Health, Bethesda, MD 20892.
Weizman
R
R
Kook
K A
KA
Vocci
F
F
Deutsch
S I
SI
Paul
S M
SM
D000315:Adrenalectomy; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism; D015536:Down-Regulation; D005442:Flumazenil / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009928:Organ Specificity; D011963:Receptors, GABA-A / Q000378:metabolism*; D012016:Reference Values; D013312:Stress, Physiological / Q000503:physiopathology*; D013550:Swimming
D011963:Receptors, GABA-A; D005442:Flumazenil