c-fos protein-like immunoreactivity in cultured human glial cells.
The expression of the c-fos protein(s) in cultured glial cells of the human brain and sympathetic ganglia was studied immunocytochemically using an antibody to a synthetic fragment of the c-fos protein (M-peptide). GFAP (glial fibrillary acidic protein) and S-100 antibodies were used as glial cell markers. The c-fos immunoreactivity (c-fos IR) was observed in both brain and peripheral ganglion derived cells maintained 7-22 days in culture. In general, c-fos IR was stronger in small spindle-shaped cells than in large cells. Deprivation of serum for 24 h decreased drastically c-fos IR in both types of cultures. The results demonstrate a serum-dependent expression of c-fos in the human glial cells derived from central and peripheral nervous systems.
Koistinaho
J
J
Department of Public Health, University of Tampere, Finland.
D001921:Brain / Q000166:cytology; D002478:Cells, Cultured; D003470:Culture Media; D005333:Fetus; D005728:Ganglia, Sympathetic / Q000166:cytology; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006801:Humans; D009457:Neuroglia / Q000166:cytology*; D011505:Protein-Tyrosine Kinases / Q000032:analysis; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D016760:Proto-Oncogene Proteins c-fos; D009418:S100 Proteins / Q000032:analysis
D003470:Culture Media; D005904:Glial Fibrillary Acidic Protein; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D009418:S100 Proteins; D011505:Protein-Tyrosine Kinases