title: Phosphatidylcholine breakdown in HDL3 stimulated platelets.
abstract: Low concentrations of HDL3 stimulate a transient biphasic increase in 1,2-diacylglycerol (DAG), with an early phase peaking at 30 seconds and a late phase at 60 seconds in (3H)-phosphatidylcholine prelabelled platelets. DAG generation is coupled to apolipoprotein AII or AI binding to specific surface receptors. Coincubations with HDL3 and 0.2 microM phorbol ester induced a significant rise in the second phase DAG indicating the involvement of protein kinase C in this late phase. The HDL3 induced production of DAG in platelets pretreated with 6 microM R 59022 is enhanced, while phosphatidic acid (PA) content was reduced, suggesting that DAG attenuation is derived at least in part from a pathway involving DAG-Kinase.
authors.affiliation: Institut Pasteur, Lille, France.
authors.forename: H
authors.initials: H
authors.lastname: Nazih
authors.forename: D
authors.initials: D
authors.lastname: Devred
authors.forename: F
authors.initials: F
authors.lastname: Martin-Nizard
authors.forename: J C
authors.initials: JC
authors.lastname: Fruchart
authors.forename: C
authors.initials: C
authors.lastname: Delbart
mesh_terms: D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D019852:Diacylglycerol Kinase; D004075:Diglycerides / Q000096:biosynthesis; D006801:Humans; D066298:In Vitro Techniques; D008075:Lipoproteins, HDL / Q000302:isolation & purification / Q000502:physiology*; D053440:Lipoproteins, HDL3; D010712:Phosphatidic Acids / Q000494:pharmacology; D010713:Phosphatidylcholines / Q000097:blood*; D010770:Phosphotransferases / Q000378:metabolism; D011493:Protein Kinase C / Q000494:pharmacology; D013110:Sphingosine / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: C011439:1,2-diacylglycerol; D004075:Diglycerides; D008075:Lipoproteins, HDL; D053440:Lipoproteins, HDL3; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D010770:Phosphotransferases; D019852:Diacylglycerol Kinase; D011493:Protein Kinase C; D013110:Sphingosine; D013755:Tetradecanoylphorbol Acetate