Preparation and evaluation of octadecyl-treated porous glasses. Application to the determination of methotrexate in serum.
The retention and selectivity of methotrexate (MTX) in serum were studied by high-performance liquid chromatography on octadecyl-treated porous glasses and silicas. From elemental analysis data for carbon, the maximum number of bonded octadecyl surface groups per gram (mean pore diameter 153 A, specific surface area 193 m2/g, pore volume 0.83 ml/g) in octadecyl-treated glass was calculated to be 0.131 x 10(21). MTX in human serum could be separated on both glasses and silicas with methanol-acetate buffer mixtures as eluents.
Okamoto
M
M
Gifu Prefectural Tajimi Hospital, Japan.
Yoshida
I
I
Utsumi
M
M
Nobuhara
K
K
Jinno
K
K
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D005898:Glass; D006801:Humans; D008727:Methotrexate / Q000097:blood; D012822:Silicon Dioxide
D012822:Silicon Dioxide; D008727:Methotrexate