Activation of excitatory amino acid receptors increases intracellular free [3H]arachidonate levels in cultured cerebellar neurons.
Addition of glutamate to cultured cerebellar neurons increases both intra- and extracellular content of [3H]arachidonic acid. The increase is time- and concentration-dependent and is antagonized by Mg2+ or by specific NMDA receptor antagonists (such as a-aminoadipate or MK-801). An increase in intracellular content of [3H]arachidonic acid is also induced by kainate and the muscarinic receptor agonist, carbamylcholine. However, as opposed to glutamate, carbamylcholine does not enhance the extracellular levels of [3H]arachidonic acid.
Aronica
E
E
Institute of Pharmacology, University of Catania School of Medicine, Italy.
Nicoletti
F
F
Canonico
P L
PL
D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology*; D008564:Membrane Potentials / Q000502:physiology; D009474:Neurons / Q000502:physiology; D051381:Rats; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface / Q000502:physiology*; D017470:Receptors, Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter / Q000502:physiology
D001095:Arachidonic Acids; D017459:Receptors, Amino Acid; D011956:Receptors, Cell Surface; D017470:Receptors, Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; D017981:Receptors, Neurotransmitter; D016718:Arachidonic Acid