Erythromycin and azithromycin transport into Haemophilus influenzae ATCC 19418 under conditions of depressed proton motive force (delta mu H).
The effect of collapsing the electrochemical proton gradient (delta mu H) on [3H]erythromycin and [14C]azithromycin transport in Haemophilus influenzae ATCC 19418 was studied. The proton gradient and membrane potential were determined from the distribution of [2-14C]dimethadione and rubidium-86, respectively. delta mu H was reduced from 124 to 3 mV in EDTA-valinomycin-treated cells at 22 degrees C with 150 mM KCl and 0.1 mM carbonyl cyanide m-chlorophenylhydrazone. During the collapse of delta mu H, macrolide uptake increased. Erythromycin efflux studies strongly suggested that this increase was not due to an energy-dependent efflux pump but was likely due to increased outer membrane permeability. These data indicated that macrolide entry was not a delta mu H-driven active transport process but rather a passive diffusion process.
Capobianco
J O
JO
Anti-Infective Research Division, Abbott Laboratories, Illinois 60064-3500.
Goldman
R C
RC
D017963:Azithromycin; D001426:Bacterial Proteins / Q000096:biosynthesis; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D004917:Erythromycin / Q000031:analogs & derivatives* / Q000493:pharmacokinetics*; D006193:Haemophilus influenzae / Q000378:metabolism* / Q000648:ultrastructure; D008564:Membrane Potentials / Q000502:physiology; D011522:Protons; D014634:Valinomycin / Q000494:pharmacology
D001426:Bacterial Proteins; D011522:Protons; D014634:Valinomycin; D004917:Erythromycin; D017963:Azithromycin; D004492:Edetic Acid