[Tissue protection by elimination of oxygen free radicals in the post-ischemic reperfusion phase].
Prolonged ischemia decreases the chances for a successful pedicled or free tissue transfer as well as for major limb replantation. Skeletal muscle is especially sensitive to periods of prolonged ischemia and reperfusion. It is now hypothesized that tissue injury occurs during reperfusion due to the formation of toxic oxygen-free radicals. A replantation model, using the rabbit hind limb tibialis anterior muscle, was developed, to assess muscle function and histological appearance following ischemic intervals of five and eight hours. Muscle strength five weeks after injury was used as a functional measurement of tissue damage. The effects of the superoxide free radical scavenger superoxide dismutase (SOD), the hydroxide radical scavenger dimethylsulfoxide (DMSO) and the xanthine-oxidase inhibitor allopurinol (ALLO), administered systemically just before reperfusion, were studied.
Feller
A M
AM
Abteilung fur Plastische und Wiederherstellungschirurgie im Klinikum rechts der Isar der Technischen Universität, München.
Roth
A C
AC
Russell
R C
RC
D000493:Allopurinol / Q000008:administration & dosage*; D000818:Animals; D004121:Dimethyl Sulfoxide / Q000008:administration & dosage*; D005609:Free Radicals; D006614:Hindlimb / Q000098:blood supply; D008854:Microscopy, Electron; D009119:Muscle Contraction / Q000187:drug effects; D009132:Muscles / Q000098:blood supply* / Q000473:pathology; D010865:Pilot Projects; D011292:Premedication; D011817:Rabbits; D015427:Reperfusion Injury / Q000517:prevention & control*; D012092:Replantation / Q000379:methods*; D013482:Superoxide Dismutase / Q000008:administration & dosage*; D013481:Superoxides / Q000378:metabolism*
D005609:Free Radicals; D013481:Superoxides; D000493:Allopurinol; D013482:Superoxide Dismutase; D004121:Dimethyl Sulfoxide
Gewebeprotektion durch Elimination von freien Sauerstoffradikalen in der postischämischen Reperfusionsphase.