Altered phosphatidylcholine metabolism in C3H10T1/2 cells transfected with the Harvey-ras oncogene.
The effect of expression of the Harvey-ras oncogene on phosphatidylcholine metabolism in C3H10T1/2 mouse fibroblast cells was examined. There were multiple changes in the CDP-choline pathway for phosphatidylcholine biosynthesis in the ras-expressing cells. The activity of the first enzyme in the pathway, choline kinase, was stimulated 1.9-fold, while the activity of the second enzyme, CTP:phosphocholine cytidylyltransferase, was decreased by one-half. High levels of intracellular phosphocholine measured in the ras cells were consistent with the altered activities of choline kinase and cytidylyltransferase. The overall rate of phosphatidylcholine synthesis appeared to be increased because the turnover rate of phosphocholine from the intracellular pool was higher in the ras-transfected cells. There also appeared to be an increased rate of phosphatidylcholine degradation in ras-expressing C3H10T1/2 cells. Very high levels of glycerophosphocholine (6-fold increased over control cells) suggested that phospholipase A was activated in these cells. These results indicate that the ras oncogene product directly or indirectly causes an increased turnover of phosphatidylcholine in C3H10T1/2 cells.
Teegarden
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D
Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907.
Taparowsky
E J
EJ
Kent
C
C
D000818:Animals; D002250:Carbon Radioisotopes; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002794:Choline / Q000378:metabolism; D002797:Choline Kinase / Q000378:metabolism; D003566:Cytidine Diphosphate Choline / Q000378:metabolism; D002798:Diacylglycerol Cholinephosphotransferase / Q000378:metabolism; D011905:Genes, ras; D007700:Kinetics; D051379:Mice; D010713:Phosphatidylcholines / Q000378:metabolism*; D010743:Phospholipids / Q000096:biosynthesis; D011865:Radioisotope Dilution Technique; D014162:Transfection; D014316:Tritium
D002250:Carbon Radioisotopes; D010713:Phosphatidylcholines; D010743:Phospholipids; D014316:Tritium; D003566:Cytidine Diphosphate Choline; D002797:Choline Kinase; D002798:Diacylglycerol Cholinephosphotransferase; D002794:Choline