title: Presence and type specificity of papillomavirus antibodies demonstrable by immunoelectron microscopy tests in samples from patients with warts.
abstract: Sera from 75 patients with warts were tested for antibodies against human papillomaviruses (HPVs) in an immunoelectron microscopy test. A group of 40 patients' sera were tested against a pooled suspension of the HPVs extracted from their warts and 90% of the sera were found to be antibody-positive. Prior to serological investigation viruses from a second group of 35 patients were typed by dot blot hybridization with HPV-1 and HPV-2 DNA. According to these results the patients were divided into type 1, type 2 and non-1, non-2 virus carriers. Sera of these patients were first tested for antibodies reactive with their homologous viruses and then for antibodies against type 1 virus. More than 80% of the patients possessed antibodies against their homologous viruses, but only a small minority of sera from type 2 and non-1, non-2 virus carriers were reactive with type 1 viruses. These results suggest that the antibody reactive in the immunoaggregation test is type-specific.
authors.affiliation: Department of Experimental Virology, Institute of Sera and Vaccines, Praha, Czechoslovakia.
authors.forename: E
authors.initials: E
authors.lastname: Anisimová
authors.forename: P
authors.initials: P
authors.lastname: Barták
authors.forename: D
authors.initials: D
authors.lastname: Vlcek
authors.forename: I
authors.initials: I
authors.lastname: Hirsch
authors.forename: B
authors.initials: B
authors.lastname: Brichácek
authors.forename: V
authors.initials: V
authors.lastname: Vonka
mesh_terms: D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis*; D002648:Child; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000276:immunology*; D013045:Species Specificity; D014860:Warts / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral