title: Adhesion, growth and cytoskeletal characteristics of 8701-BC breast carcinoma cells cultured in the presence of type V collagen.
abstract: Type V collagen is one of the minor components of the extracellular matrix (ECM) whose content is increased in cases of ductal infiltrating carcinomas of the breast. In order to clarify its biological role, we have investigated the effect of this molecule, both as substrate and as soluble factor, on the behaviour of a breast carcinoma cell line (8701-BC) grown in vitro. Cell-collagen adhesion was monitored for 24 h from plating in the absence or presence of serum. The influence of type V collagen on cell growth was followed during 9 days of culture, and the actin-vinculin arrangement was studied by simultaneous fluorescent immuno-staining. The results indicate that type V collagen is not a permissive substrate for neoplastic cell proliferation and dissemination in vitro.
authors.affiliation: Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Luparello
authors.forename: R
authors.initials: R
authors.lastname: Schillaci
authors.forename: I
authors.initials: I
authors.lastname: Pucci-Minafra
authors.forename: S
authors.initials: S
authors.lastname: Minafra
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology* / Q000648:ultrastructure; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000473:pathology* / Q000648:ultrastructure; D002448:Cell Adhesion; D002455:Cell Division; D003094:Collagen / Q000502:physiology*; D003598:Cytoskeletal Proteins / Q000648:ultrastructure*; D006801:Humans; D014407:Tumor Cells, Cultured / Q000473:pathology / Q000648:ultrastructure
chemical_list: D003598:Cytoskeletal Proteins; D003094:Collagen