Growth inhibition of BALB/c 3T3 cells by a high-molecular-weight mucin-like glycoprotein of human milk fat globule membrane.
PAS-O is a high-molecular-weight mucin-like glycoprotein of the human milk fat globule membrane, and originates from the lactating mammary epithelial cell membrane. The cell proliferation rate of BALB/c 3T3 cells was markedly reduced by addition of PAS-O to their culture medium, this inhibition being concentration-dependent (0-10 microgram/ml) and reversible. The inhibitory activity was removed by periodate or protease treatment of the glycoprotein. PAS-O may interact with 3T3 cells to mimic the effect of high cell density on growth. PAS-O, which has been recognized as a glycoprotein molecule carrying a differentiation antigen expressed on the mammary epithelial cell surface, may play a role in regulating the growth of the mammary cells.
Shimizu
M
M
Department of Agricultural Chemistry, University of Tokyo.
Tanimoto
H
H
Azuma
N
N
Yamauchi
K
K
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D005260:Female; D006801:Humans; D008562:Membrane Glycoproteins / Q000494:pharmacology*; D051379:Mice; D008895:Milk, Human; D008970:Molecular Weight; D018396:Mucin-1; D009077:Mucins / Q000494:pharmacology*; D009439:Neuraminidase / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D010504:Periodic Acid / Q000494:pharmacology
D008562:Membrane Glycoproteins; D018396:Mucin-1; D009077:Mucins; D010504:Periodic Acid; C009288:metaperiodate; D009439:Neuraminidase; D010447:Peptide Hydrolases