title: Suppression of synthesis and release of glucagon by glucagon-like peptide-1 (7-36 amide) without affect on mRNA level in isolated rat islets.
abstract: Glucagon-like peptide-1 (GLP-1) has been reported to inhibit glucagon release. To investigate the mechanism involved, we examined the effects of GLP-1 on the preproglucagon mRNA level and the content and release of glucagon in the isolated rat islets. Arginine significantly increased the content and release of glucagon after incubation for 1 h or 18 h. The preproglucagon mRNA level was not increased after incubation for 1 h but was increased after incubation for 18 h. GLP-1 (7-36 amide) significantly decreased the content and release of glucagon after incubation for 1 h or 18 h, but did not affect arginine-induced increase in the preproglucagon mRNA level after incubation for 18 h. These data suggest that GLP-1 suppresses post-transcriptional processes in the content and release of glucagon.
authors.affiliation: Department of Geriatric Medicine, Osaka University Medical School, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Yamato
authors.forename: Y
authors.initials: Y
authors.lastname: Noma
authors.forename: Y
authors.initials: Y
authors.lastname: Tahara
authors.forename: H
authors.initials: H
authors.lastname: Ikegami
authors.forename: Y
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Cha
authors.forename: H
authors.initials: H
authors.lastname: Yoneda
authors.forename: T
authors.initials: T
authors.lastname: Ogihara
authors.forename: C
authors.initials: C
authors.lastname: Ohboshi
authors.forename: M
authors.initials: M
authors.lastname: Hirota
mesh_terms: D000818:Animals; D005934:Glucagon / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D052216:Glucagon-Like Peptide 1; D004763:Glucagon-Like Peptides; D066298:In Vitro Techniques; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D010446:Peptide Fragments; D010455:Peptides / Q000494:pharmacology*; D052336:Proglucagon; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D010446:Peptide Fragments; D010455:Peptides; D011498:Protein Precursors; D012333:RNA, Messenger; C055453:glucagon-like peptide 1 (7-36)amide; D052336:Proglucagon; D004763:Glucagon-Like Peptides; D052216:Glucagon-Like Peptide 1; D005934:Glucagon