title: Longer-term regulation of pyruvate dehydrogenase kinase in cultured rat cardiac myocytes.
abstract: The increased activity of pyruvate dehydrogenase (PDH) kinase induced in hearts of rats by starvation for 48 h was maintained following preparation of cardiac myocytes, and it was also maintained, though at a decreased level, after 25 h of culture in medium 199. This loss of PDH kinase activity was not prevented by n-octanoate, dibutyryl cyclic AMP or glucagon. The PDH kinase activity of myocytes from fed rats was increased to that of starved rats after 25 h of culture with n-octanoate, dibutyryl cyclic AMP or both agents together.
authors.affiliation: Nuffield Department of Clinical Biochemistry, John Radcliffe Hospital, Oxford, U.K.
authors.forename: D R
authors.initials: DR
authors.lastname: Marchington
authors.forename: A L
authors.initials: AL
authors.lastname: Kerbey
authors.forename: P J
authors.initials: PJ
authors.lastname: Randle
mesh_terms: D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002210:Caprylates / Q000494:pharmacology; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002478:Cells, Cultured; D003470:Culture Media; D005502:Food; D005934:Glucagon / Q000494:pharmacology; D009206:Myocardium / Q000201:enzymology*; D011494:Protein Kinases / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases; D000081382:Pyruvate Dehydrogenase Acetyl-Transferring Kinase; D051381:Rats; D013217:Starvation / Q000201:enzymology*
chemical_list: D002210:Caprylates; D003470:Culture Media; D000081382:Pyruvate Dehydrogenase Acetyl-Transferring Kinase; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D003994:Bucladesine; D005934:Glucagon; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; C031492:octanoic acid