Flow cytometric detection of bovine viral diarrhea virus in peripheral blood leukocytes of persistently infected cattle.
Flow cytometry was investigated for detection of bovine viral diarrhea virus (BVDV) in peripheral blood mononuclear leukocytes of persistently infected cattle. The mononuclear leukocytes were purified by sedimentation in a gradient of Ficoll-Paque, fixed, permeabilized, and then labelled by indirect immunofluorescence using biotinylated immunoglobulins from a porcine antiserum to BVDV. Flow cytometric analysis of blood samples obtained from persistently infected cattle revealed virus in 3.0-21.0% (mean +/- SD, 11.2% +/- 6.4%) of the mononuclear leukocytes. Fluorescent cells were not observed in controls. Flow cytometric detection of BVDV in blood cells of persistently infected bovines is a rapid and objective technique which does not require cell culture facilities.
Qvist
P
P
Animal Biotechnology Research Center, National Veterinary Laboratory, Copenhagen, Denmark.
Aasted
B
B
Bloch
B
B
Meyling
A
A
Rønsholt
L
L
Houe
H
H
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000382:microbiology*; D002417:Cattle; D002469:Cell Separation; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology / Q000302:isolation & purification*; D005260:Female; D005434:Flow Cytometry; D007963:Leukocytes, Mononuclear / Q000382:microbiology*
D000956:Antigens, Viral