title: Ouabain-insensitive, Na-ATPase activity in pure suspensions of rat kidney proximal tubules.
abstract: The present work was undertaken to evaluate the distribution of the Na-ATPase activity in the different components of the rat kidney cortex. Suspensions of glomeruli, proximal and distal tubules were prepared following a collagenase digestion of outermost kidney cortex slices and a separation on a Percoll gradient. It was found that the Na-ATPase activity is higher in the fraction enriched in proximal tubules. The fraction enriched in glomeruli and in distal tubules show also a Na-ATPase activity, but it is lower.
authors.affiliation: Centro de Biofisica y Bioquimica, Instituto Venezolano de Investigaciones Cientificas (IVIC), Caracas, Venezuela.
authors.forename: R
authors.initials: R
authors.lastname: Marín
authors.forename: D C
authors.initials: DC
authors.lastname: Gómez
authors.forename: G A
authors.initials: GA
authors.lastname: Rodríguez
authors.forename: T
authors.initials: T
authors.lastname: Proverbio
authors.forename: F
authors.initials: F
authors.lastname: Proverbio
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D066298:In Vitro Techniques; D007687:Kidney Tubules, Proximal / Q000201:enzymology*; D009994:Osmolar Concentration; D010042:Ouabain / Q000494:pharmacology*; D011205:Povidone; D051381:Rats; D011919:Rats, Inbred Strains; D012822:Silicon Dioxide; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D010042:Ouabain; C016039:Percoll; D012822:Silicon Dioxide; D000469:Alkaline Phosphatase; D000254:Sodium-Potassium-Exchanging ATPase; D011205:Povidone