Analysis of interchromosomal mitotic recombination.
A novel synthetic locus is described that provides a simple assay system for characterizing mitotic recombinants. The locus consists of the TRP1 and HIS3 genes inserted into chromosome III of S. cerevisiae between the CRY1 and MAT loci. Defined trp1 and his3 alleles have been generated that allow the selection of interchromosomal recombinants in this interval. Trp+ or His+ recombinants can be divided into several classes based on coupling of the other alleles in the interval. The tight linkage of the CRY1 and MAT loci, combined with the drug resistance and cell type phenotypes that they respectively control, facilitates the classification of the recombinants without resorting to tetrad dissection. We present the distribution of spontaneous recombinants among the classes defined by this analysis. The data suggest that the recombination intermediate can have regions of symmetric strand exchange and that co-conversion tracts can extend over 1-3 kb. Continuous conversion tracts are favored over discontinuous tracts. The distribution among the classes defined by this analysis is altered in recombinants induced by UV irradiation.
McGill
C B
CB
NCI-Frederick Cancer Research and Development Center, Laboratory of Eukaryotic Gene Expression, MD 21702-1201.
Shafer
B K
BK
Higgins
D R
DR
Strathern
J N
JN
D000483:Alleles; D004260:DNA Repair; D004251:DNA Transposable Elements; D004271:DNA, Fungal; D005785:Gene Conversion; D005800:Genes, Fungal; D008040:Genetic Linkage; D008938:Mitosis; D009630:Nondisjunction, Genetic; D010641:Phenotype; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000528:radiation effects; D014466:Ultraviolet Rays
D004251:DNA Transposable Elements; D004271:DNA, Fungal