Immunoblotting analysis of concanavalin A-isolated allergens of Candida albicans.
The carbohydrate-containing fraction of Candida albicans was isolated from the crude extract with ConA Sepharose affinity chromatography and studied by IgE-immunoblotting with individual and pooled sera from C. albicans-allergic subjects. In the ConA-bound fraction there was a diffuse IgE binding in the high molecular weight area which also gave a carbohydrate stain (PAS). A distinct band corresponding to a molecular weight of 70 kD bound specific IgE antibodies. This glycoprotein, presumably a mannoprotein, gave a weak carbohydrate staining and a strong protein staining. Further biochemical studies are needed to reveal the exact nature of the epitopes in the ConA-bound mannose-containing fraction of C. albicans.
Savolainen
J
J
Department of Medical Microbiology, University of Turku, Finland.
Viander
M
M
Einarsson
R
R
Koivikko
A
A
D000485:Allergens / Q000276:immunology*; D002176:Candida albicans / Q000276:immunology* / Q000302:isolation & purification; D003208:Concanavalin A / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007073:Immunoglobulin E / Q000378:metabolism; D008351:Mannans / Q000032:analysis* / Q000276:immunology
D000485:Allergens; D008351:Mannans; D003208:Concanavalin A; D007073:Immunoglobulin E