The localization of macrophage subsets and dendritic cells in the gastrointestinal tract of the mouse with special reference to the presence of high endothelial venules. An immuno- and enzyme-histochemical study.
This study concerns the distribution of macrophages and dendritic cells (DC) in the gastrointestinal tract of the mouse. Heterogeneity of macrophage population was found by using the MOMA-1, MOMA-2, ERTR-9, Mac-1 and F4/80 monoclonal antibodies. MOMA-1, ERTR-9, Mac-1 and F4/80+ cells were detected mostly at the villous cores in the lamina propria of the villi, whereas MOMA-2+ cells were primarily found around the crypts at the base of the villi. These MOMA-2+ cells revealed a granular appearance throughout the cytoplasm and displayed a strong acid phosphatase (AcPh) activity. Few MOMA-2+ cells were seen at the top of the villi in the epithelium. Although MOMA-1 and ERTR-9+ cells have similar morphology and the same distribution patterns in the lamina propria, they are likely different populations, because in Peyer's patches (PP), MOMA-1+ cells were present, whereas ERTR-9+ cells could not be detected. Both populations displayed AcPh activity. Strongly stained Mac-1+ cells were abundantly seen in the lamina propria of the small intestine. F4/80+ cells were rare. NLDC-145+ cells with AcPh activity and weak Ia staining were also found. In the PP-associated villi and in the T-dependent area of PP, dendritic NLDC-145+ cells, which were strongly Ia positive, were detected. MIDC-8+ cells were found only in the T-dependent area. Few NLDC-145+ cells (dendritic cells) were found in the upper part of the oesophagus. These cells were also stained with the MIDC-8 antibody.(ABSTRACT TRUNCATED AT 250 WORDS)
Soesatyo
M
M
Department of Histology, Medical Faculty, Vrije Universiteit, Amsterdam, The Netherlands.
Biewenga
J
J
Kraal
G
G
Sminia
T
T
D000818:Animals; D000911:Antibodies, Monoclonal; D003713:Dendritic Cells / Q000166:cytology*; D004064:Digestive System / Q000098:blood supply / Q000166:cytology / Q000276:immunology*; D004730:Endothelium, Vascular / Q000276:immunology; D004947:Esophagus / Q000276:immunology; D007124:Immunoenzyme Techniques; D007413:Intestinal Mucosa / Q000276:immunology; D007420:Intestine, Large / Q000098:blood supply / Q000276:immunology; D007421:Intestine, Small / Q000098:blood supply / Q000276:immunology; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010581:Peyer's Patches / Q000276:immunology; D013270:Stomach / Q000098:blood supply / Q000276:immunology; D014699:Venules / Q000276:immunology
D000911:Antibodies, Monoclonal