Expression of the human papillomavirus type 16 genome in SK-v cells, a line derived from a vulvar intraepithelial neoplasia.
The SK-v cells, established from a premalignant vulvar lesion, contain human papillomavirus type 16 (HPV-16) sequences integrated at a single cellular site and derive from a cell clone present in vivo. Transcription of the HPV-16 genome in SK-v cells was analysed by cDNA heteroduplex mapping and sequencing, and by RNase mapping. Viral sequences were shown to be transcribed into virus-cell fusion messengers. The two major transcripts have a coding capacity for a truncated E6 protein, an E7 protein and an E1-E4 fusion protein, but differ in their 3' virus-cell junction. Minor transcripts have a coding capacity for a full-length E6 protein and another truncated version of E6. The transcription pattern in the E6-E7 region was found to be the same both in SK-v cells and in CaSki cells, a line derived from an invasive cervical carcinoma. Immunoprecipitation experiments showed that the E6 protein (18K) and, predominantly, the E7 protein (20K) are expressed in SK-v cells as in CaSki cells. The E7 protein was found in a two- to threefold lower amount in SK-v cells, but showing the same half-life (about 1 h).
Schneider-Maunoury
S
S
Unité des Papillomavirus, INSERM U190, Institut Pasteur, Paris, France.
Pehau-Arnaudet
G
G
Breitburd
F
F
Orth
G
G
D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000382:microbiology; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005091:Exons; D005260:Female; D006801:Humans; D015603:Keratinocytes; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics*; D010957:Plasmids; D011230:Precancerous Conditions / Q000382:microbiology*; D011233:Precipitin Tests; D015347:RNA Probes; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000382:microbiology; D014764:Viral Proteins / Q000096:biosynthesis; D014846:Vulvar Neoplasms / Q000382:microbiology*
D004279:DNA, Viral; D015347:RNA Probes; D014764:Viral Proteins