Permeabilized cells as a way of gaining access to intracellular organelles: an approach to glycosylation reactions.
The selective plasma membrane permeabilization of animal cells is a way of introducing non permeable substrates into the cytoplasmic space. This technique facilitates the introduction of a wide range of labelled precursors and avoids the drawbacks of subcellular fractionation. We review here various physical and chemical methods successfully used in different metabolic studies, and as an example, note the advantages of permeabilized cells in glycosylation studies.
Lepers
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Laboratoire de Chimie Biologique, Université des Sciences et Techniques de Lille-Flandres-Artois, Unité Mixte du CNRS No 111, Villeneuve-d'Ascq, France.
Cacan
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Verbert
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D000818:Animals; D002463:Cell Membrane Permeability; D004072:Digitonin / Q000494:pharmacology; D006027:Glycosides / Q000494:pharmacology*; D006031:Glycosylation; D006168:Guinea Pigs; D010222:Parainfluenza Virus 1, Human / Q000502:physiology; D012503:Saponins / Q000494:pharmacology
D006027:Glycosides; D012503:Saponins; D004072:Digitonin