title: Adenosine blocks calcium entry in activated neutrophils and binds to flunarizine-sensitive calcium channels.
abstract: Adenosine is able to prevent Ca+(+) influx into activated neutrophils as detected by the specific fluorescent indicator Quin 2. Such an effect is shown in a similar fashion by the calcium entry blocker flunarizine. The binding interaction between flunarizine and the neutrophil membrane as well as the flunarizine-adenosine antagonism are shown by the 1H-NMR technique, thus supporting evidence of a competition between the agents at the same or a nearby site on the cell membrane.
authors.affiliation: Institute of Medical Pathology, University of Siena, Policlinico, Le Scotte, Italy.
authors.forename: F L
authors.initials: FL
authors.lastname: Pasini
authors.forename: P L
authors.initials: PL
authors.lastname: Capecchi
authors.forename: A L
authors.initials: AL
authors.lastname: Pasqui
authors.forename: L
authors.initials: L
authors.lastname: Ceccatelli
authors.forename: T
authors.initials: T
authors.lastname: Di Perri
authors.forename: G
authors.initials: G
authors.lastname: Valensin
authors.forename: E
authors.initials: E
authors.lastname: Gaggelli
mesh_terms: D000241:Adenosine / Q000378:metabolism / Q000494:pharmacology*; D000634:Aminoquinolines; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism; D005444:Flunarizine / Q000494:pharmacology; D005456:Fluorescent Dyes; D006801:Humans; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*
chemical_list: D000634:Aminoquinolines; D015220:Calcium Channels; D005456:Fluorescent Dyes; D009240:N-Formylmethionine Leucyl-Phenylalanine; D000241:Adenosine; C035739:Quin2; D005444:Flunarizine; D002118:Calcium