Determination of serum cholinesterase activity and dibucaine numbers by an amperometric choline sensor.
Serum cholinesterase activity and the dibucaine numbers have been determined by using a hydrogen peroxide electrode and the enzyme choline oxidase immobilized on a nylon net. The analysis procedure is extremely simple and very fast allowing 30 cholinesterase determinations per hour. Both cholinesterase activity and dibucaine number measurements could be performed in 5 min and by using serum samples of only 10 microliters. When used in sera the probe showed no interference from electroactive compounds present in blood, and also showed good stability and reproducibility. These features make this sensor appropriate for continuous extracorporeal circuit blood monitoring of succinylcholine during surgery.
Palleschi
G
G
Dipartimento di Scienze e Tecnologie Chimiche Universita' di Roma, Italy.
Lavagnini
M G
MG
Moscone
D
D
Pilloton
R
R
D'Ottavio
D
D
Evangelisti
M E
ME
D000429:Alcohol Oxidoreductases; D015374:Biosensing Techniques; D002794:Choline; D002800:Cholinesterase Inhibitors; D002802:Cholinesterases / Q000097:blood*; D003992:Dibucaine / Q000494:pharmacology*; D004800:Enzymes, Immobilized; D006801:Humans; D006861:Hydrogen Peroxide
D002800:Cholinesterase Inhibitors; D004800:Enzymes, Immobilized; D006861:Hydrogen Peroxide; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D002802:Cholinesterases; D003992:Dibucaine; D002794:Choline