Malignant transformation of human endometrial stromal cells by transfection of c-myc: effects of pRSVneo cotransfection and treatment with MNNG.
Stromal cells isolated from normal human endometrium were cotransfected in primary culture with pSVc-myc, a plasmid containing a truncated c-myc gene regulated by simian virus 40 promoter, and pRSV neo, a plasmid containing a neomycin resistance gene regulated by Rous sarcoma virus (RSV) promoter. These cells demonstrated properties of transformed cells in vitro, including altered morphology, focus formation, anchorage-independent growth, chromosomal alterations, and tumor formation in athymic mice. When these cells were treated subsequently with a direct-acting carcinogen, N-methyl-N'-nitro-N-nitrosoguanidine, they demonstrated higher colony-forming efficiency in soft agar and reduced tumor latency. Cells transfected with pRSV neo alone exhibited some properties associated with neoplastic transformation, including altered morphology and formation of colonies in soft agar. It is presumed that in normal cells transfected with pRSV neo, RSV long terminal repeats activated cellular genes that normally regulate growth of human endometrial stromal cells.
Lyn-Cook
B D
BD
Department of Pathology, University of North Carolina, School of Medicine, Chapel Hill.
Siegal
G P
GP
Kaufman
D G
DG
D001358:Avian Sarcoma Viruses / Q000235:genetics; D002448:Cell Adhesion / Q000187:drug effects; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000235:genetics / Q000473:pathology*; D004351:Drug Resistance / Q000235:genetics; D004717:Endometrium / Q000187:drug effects / Q000473:pathology*; D005260:Female; D006801:Humans; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009355:Neomycin / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000502:physiology*; D014162:Transfection / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D014170:Transformation, Genetic / Q000187:drug effects / Q000502:physiology*; D014594:Uterine Neoplasms / Q000235:genetics / Q000473:pathology
D016271:Proto-Oncogene Proteins c-myc; D008769:Methylnitronitrosoguanidine; D009355:Neomycin