[Study of the structure of amplifying sequence of Streptomyces antibioticus].
A low productive laboratory strain of S. antibioticus and a strain with an increased productivity of oleandomycin derived from it were studied comparatively with using restriction analysis and blotting hybridization. Amplification, site specific integration and segregation of the DNA sequence 32.0 kb in size were detected in the strains. The chromosomes of the laboratory strain contained one copy of the amplifying sequence AUD. After uniting of the end sequences AUD appeared to be capable of segregating from the chromosomes and its one copy per five genomes was present in the form of an extrachromosomal genetic element eSA1. The genome of the strain with increased productivity of oleandomycin contained in its chromosomes sequence ADS-Sa1 amplified to 150 copies and the eSA1 extrachromosomal genetic element in the form of mono-, di- and trimeric structures in the quantity of approximately one copy per genome. The BamHIB fragment of the eSA1 DNA 4 kb in size was identified. The fragment was able to participate in segregation or integration of eSA1 from or into the chromosomes since its subfragments were flanking AUD and ADS-SA1 in the chromosomes. The BamHIB fragment was hybridizing with a number of fragments of the chromosomal DNA of S. antibioticus, S. erythraeus. S. lividans and other strains of streptomycetes. It probably contained an IS-like element or a dispersed genetic element of another class. The DNA sequence of the eSA1 genetic element contained regions homologous to the sequence of the Erm E gene in S. erythraeus NRRL 2338.
Ukhabotina
L S
LS
Danilenko
V N
VN
D015139:Blotting, Southern / Q000379:methods; D003470:Culture Media; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics*; D066298:In Vitro Techniques; D008957:Models, Genetic; D009693:Nucleic Acid Hybridization / Q000187:drug effects / Q000502:physiology*; D009827:Oleandomycin / Q000096:biosynthesis; D012091:Repetitive Sequences, Nucleic Acid / Q000187:drug effects / Q000502:physiology*; D013302:Streptomyces / Q000235:genetics*; D013303:Streptomyces antibioticus / Q000235:genetics* / Q000378:metabolism
D003470:Culture Media; D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes; D009827:Oleandomycin
Izuchenie struktury amplifitsiruiushcheĭsia posledovatel'nosti Streptomyces antibioticus.