Pyrophosphatase is essential for growth of Escherichia coli.
The ppa gene for inorganic pyrophosphatase is essential for the growth of Escherichia coli. A recombinant with a chromosomal ppa::Kanr lesion and a temperature-sensitive replicon with a ppa+ gene showed a temperature-sensitive growth phenotype, and a mutant with the sole ppa+ gene under control of the lac promoter showed inducer-dependent growth. When the lacp-ppa mutant was subcultured without inducer, the pyrophosphatase level decreased, the PPi level increased, and growth stopped. Cellular PPi reached 16 mM about 6 h after growth arrest without loss of cell viability.
Chen
J
J
Laboratoire de Biochimie, 240 du Centre National de la Recherche Scientifique, Ecole Polytechnique, Palaiseau, France.
Brevet
A
A
Fromant
M
M
Lévêque
F
F
Schmitter
J M
JM
Blanquet
S
S
Plateau
P
P
D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D005798:Genes, Bacterial; D005838:Genotype; D043564:Inorganic Pyrophosphatase; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010641:Phenotype; D010957:Plasmids; D011755:Pyrophosphatases / Q000235:genetics* / Q000378:metabolism; D012093:Replicon; D015183:Restriction Mapping; D013696:Temperature
D004269:DNA, Bacterial; D015345:Oligonucleotide Probes; D011755:Pyrophosphatases; D043564:Inorganic Pyrophosphatase