title: Gelsolin-actin interaction and actin polymerization in human neutrophils.
abstract: The fraction of polymerized actin in human blood neutrophils increases after exposure to formyl-methionyl-leucyl-phenylalanine (fmlp), is maximal 10 s after peptide addition, and decreases after 300 s. Most of the gelsolin (85 +/- 11%) in resting ficoll-hypaque (FH)-purified neutrophils is in an EGTA resistant, 1:1 gelsolin-actin complex, and, within 5 s after 10(-7) M fmlp activation, the amount of gelsolin complexed with actin decreases to 42 +/- 12%. Reversal of gelsolin binding to actin occurs concurrently with an increase in F-actin content, and the appearance of barbed-end nucleating activity. The rate of dissociation of EGTA resistant, 1:1 gelsolin-actin complexes is more rapid in cells exposed to 10(-7) M fmlp than in cells exposed to 10(-9) M fmlp, and the extent of dissociation 10 s after activation depends upon the fmlp concentration. Furthermore, 300 s after fmlp activation when F-actin content is decreasing, gelsolin reassociates with actin as evidenced by an increase in the amount of EGTA resistant, 1:1 gelsolin-actin complex. Since fmlp induces barbed end actin polymerization in neutrophils and since in vitro the gelsolin-actin complex caps the barbed ends of actin filaments and blocks their growth, the data suggests that in FH neutrophils fmlp-induced actin polymerization could be initiated by the reversal of gelsolin binding to actin and the uncapping of actin filaments or nuclei. The data shows that formation and dissociation of gelsolin-actin complexes, together with the effects of other actin regulatory proteins, are important steps in the regulation of actin polymerization in neutrophils. Finally, finding increased amounts of gelsolin-actin complex in basal FH cells and dissociation of the complex in fmlp-activated cells suggests a mechanism by which fmlp can cause actin polymerization without an acute increase in cytosolic Ca++.
authors.affiliation: Department of Pediatrics, University of Alabama, Birmingham 35233.
authors.forename: T
authors.initials: T
authors.lastname: Howard
authors.forename: C
authors.initials: C
authors.lastname: Chaponnier
authors.forename: H
authors.initials: H
authors.lastname: Yin
authors.forename: T
authors.initials: T
authors.lastname: Stossel
mesh_terms: D000199:Actins / Q000378:metabolism* / Q000502:physiology; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000378:metabolism*; D002457:Cell Extracts; D004305:Dose-Response Relationship, Drug; D004533:Egtazic Acid / Q000494:pharmacology; D018260:Gelsolin; D006801:Humans; D008840:Microfilament Proteins / Q000032:analysis / Q000378:metabolism*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D011108:Polymers / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
chemical_list: D000199:Actins; D002135:Calcium-Binding Proteins; D002457:Cell Extracts; D018260:Gelsolin; D008840:Microfilament Proteins; D011108:Polymers; D004533:Egtazic Acid; D009240:N-Formylmethionine Leucyl-Phenylalanine