Inhibition of HSV-1 multiplication in rat embryo fibroblasts constitutively expressing the EJ-ras oncogene.
In order to examine cellular gene involvement in HSV-1 expression, we constructed different rat embryo fibroblast cell lines immortalized by adenovirus E1A or c-myc, with or without the human EJ bladder carcinoma transforming oncogene EJ-ras. HSV-1 multiplication was strongly inhibited in cells expressing EJ-ras genes compared to immortalized control cells. Virus adsorption and penetration were not quantitatively modified, but HSV-1 DNA replication was inhibited. The expression of viral thymidine kinase (TK) activity after infection by recombinant virus with the TK coding sequence under immediate-early (IE) promoter control showed that IE gene expression is inhibited in cells expressing EJ-ras. Analysis of IE gene transcription by Northern-blot hybridization and by nuclear run-off transcription assay indicates that this inhibition takes place at the transcriptional level.
Garcin
D
D
Ecole Normale Supérieure de Lyon, INSERM CJF 88-11, France.
Michal
Y
Y
Jault
F
F
Lyon
M
M
Lenoir
G
G
Jacquemont
B
B
D017456:Adenovirus Early Proteins; D000260:Adenoviruses, Human / Q000235:genetics; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D004261:DNA Replication; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology; D005814:Genes, Viral; D011905:Genes, ras; D006801:Humans; D009693:Nucleic Acid Hybridization; D009856:Oncogene Proteins, Viral / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D011916:Rats, Inbred F344; D015183:Restriction Mapping; D018139:Simplexvirus / Q000502:physiology*; D013937:Thymidine Kinase / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection; D014709:Vero Cells; D014779:Virus Replication
D017456:Adenovirus Early Proteins; D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger; D013937:Thymidine Kinase