Characterization and expression of a glycoprotein encoded by the Epstein-Barr virus BamHI I fragment.
Computer-assisted analysis of the Epstein-Barr virus (EBV) open reading frame BILF2 (B95-8 nucleotides 150,525 to 149,782) predicts that it codes for a membrane-bound glycoprotein. [3H]glucosamine labeling of cells infected with vaccinia virus recombinants that expressed the BILF2 open reading frame revealed several diffuse species of glycoproteins of around 80,000 and 55,000 daltons. A monoclonal antibody derived from spleens of mice immunized with EBV immunoprecipitated the EBV-derived protein made by the vaccinia virus recombinants and also precipitated a late envelope glycoprotein with a mobility of 78,000 to 55,000 from EBV-producing cells. N-Glycanase treatment of the immunoprecipitated BILF2 product from EBV-producing cells resulted in a polypeptide of 28 kilodaltons, closely agreeing with the predicted molecular mass for the unmodified BILF2 gene product. Western (immuno-) blots using recombinant infected cells as a source of antigen showed that the majority of EBV-seropositive individuals have a serum antibody response to the BILF2-encoded gp78/55.
Mackett
M
M
Cancer Research Campaign Laboratories, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, United Kingdom.
Conway
M J
MJ
Arrand
J R
JR
Haddad
R S
RS
Hutt-Fletcher
L M
LM
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D015245:Deoxyribonuclease BamHI; D005455:Fluorescent Antibody Technique; D005814:Genes, Viral; D006023:Glycoproteins / Q000235:genetics*; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping; D014616:Vaccinia virus / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D007074:Immunoglobulin G; D014764:Viral Proteins; D015245:Deoxyribonuclease BamHI