title: Local and systemic control of myometrial contractile activity during labour in the sheep.
abstract: The relative contribution of systemic versus local (intrauterine) factors in the activation and stimulation of the sheep myometrium during labour was examined using an in-vivo myometrial explant preparation. Myometrial tissue alone (MYO) or with attached endometrium (ENDO/MYO) was removed from the pregnant uterine horn, sutured to a stainless-steel frame and placed into the omental fat. After 7-10 days the explants developed a pattern of electromyographic activity qualitatively similar to that of the uterine myometrium. Induction of preterm labour by infusion of ACTH (66.6 ng/min for 15 min every 2 h) to the fetus resulted in a reduction in plasma progesterone concentrations and increases in values of oestradiol-17 beta and 13,14-dihydro 15-keto PGF-2 alpha in maternal plasma. The onset of labour, which followed these endocrine changes, was characterized by an increase in EMG burst frequency and reduction in burst duration occurring simultaneously in both the uterine myometrium and in the explants. The response of the uterine and explant myometrium to oxytocin also exhibited a parallel significant increase over the 24-h period leading to delivery. No differences were apparent between the explants containing myometrial tissue alone or those comprising endometrial and myometrial tissue. There was no significant change in uterine or explant EMG activity, or oxytocin responsiveness, after saline administration to the fetus. The pattern of EMG activity changes during spontaneous labour were not distinguishable from those during ACTH-induced labour. As with oxytocin, the responsiveness of the explants to electrical stimulation increased significantly at labour compared to pre-labour. These data suggest that factors within the systemic circulation play a major role in both the onset of labour contractions and the increased response to electrical or hormonal (oxytocin) stimulation during parturition in sheep.
authors.affiliation: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, London, Ontario, Canada.
authors.forename: S J
authors.initials: SJ
authors.lastname: Lye
authors.forename: C L
authors.initials: CL
authors.lastname: Freitag
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D015237:Dinoprost / Q000031:analogs & derivatives / Q000097:blood; D004576:Electromyography; D004717:Endometrium / Q000502:physiology; D004958:Estradiol / Q000097:blood; D005260:Female; D007751:Labor, Induced; D007743:Labor, Obstetric / Q000502:physiology*; D009215:Myometrium / Q000187:drug effects / Q000502:physiology*; D010121:Oxytocin / Q000494:pharmacology; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D012756:Sheep / Q000502:physiology*; D016378:Tissue Transplantation; D014590:Uterine Contraction / Q000502:physiology*
chemical_list: C008228:15-keto-13,14-dihydroprostaglandin F2alpha; D011374:Progesterone; D004958:Estradiol; D010121:Oxytocin; D000324:Adrenocorticotropic Hormone; D015237:Dinoprost