Mutagenesis of acetobacter methanolicus MB58 with the transposon Tn5.
Transposon mutagenesis was applied to the isolation of mutants of the facultatively methylotrophic Acetobacter methanolicus MB 58. The transposon Tn5 (pSU2011) was transferred from Escherichia coli SM 10 by means of conjugation to Acetobacter methanolicus MB 58. Four out of 1850 stable Km-resistant transconjugants were identified that were formaldehyde sensitive and failed to grow on methanol.
Dobrowolski
P
P
Academy of Sciences of the G.D.R., Institute of Biotechnology, Leipzig.
Gründig
M W
MW
D000091:Acetobacter / Q000235:genetics*; D004251:DNA Transposable Elements; D009154:Mutation
D004251:DNA Transposable Elements