title: Cell culture propagation of a coronavirus isolated from cows with winter dysentery.
abstract: Fecal filtrates from cows with winter dysentery were inoculated into gnotobiotic and conventional calves, and a coronavirus was isolated from calf feces. Cytopathic effects were observed on human rectal tumor cells but not bovine cell cultures. The winter dysentery isolates morphologically and antigenically resembled the Mebus strain of bovine coronavirus.
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.
authors.forename: D A
authors.initials: DA
authors.lastname: Benfield
authors.forename: L J
authors.initials: LJ
authors.lastname: Saif
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D003332:Coronaviridae / Q000254:growth & development* / Q000302:isolation & purification; D003333:Coronaviridae Infections / Q000150:complications / Q000662:veterinary*; D003588:Cytopathogenic Effect, Viral; D004403:Dysentery / Q000150:complications / Q000662:veterinary*; D005856:Germ-Free Life; D006801:Humans; D014407:Tumor Cells, Cultured; D014776:Virus Cultivation