title: Human malignant melanoma cell line (HMV-II) for isolation of influenza C and parainfluenza viruses.
abstract: HMV-II, a human malignant melanoma cell line, was compared with other cell lines (MDCK, Vero, and LLC-MK2) and primary cultures of monkey kidney (PMK) cells for the isolation and quantification of influenza and parainfluenza viruses. HMV-II cells were superior to MDCK and LLC-MK2 cells in quantification of the influenza C virus and were used successfully in the isolation of the virus from clinical specimens. The HMV-II cell line was also more sensitive for isolating parainfluenza viruses from clinical specimens than were Vero and PMK cells; there was, however, no significant difference in the quantification of the viruses among these cultures. As far as influenza A and B viruses were concerned, the HMV-II cell line was significantly less sensitive than MDCK cells, and no virus was isolated from clinical specimens with HMV-II cells. Thus, HMV-II cells are useful for the isolation of influenza C and parainfluenza viruses as an alternative to embryonated hen's eggs and PMK cells.
authors.affiliation: Virus Center, Sendai National Hospital, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Moriuchi
authors.forename: T
authors.initials: T
authors.lastname: Oshima
authors.forename: H
authors.initials: H
authors.lastname: Nishimura
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: N
authors.initials: N
authors.lastname: Katsushima
authors.forename: Y
authors.initials: Y
authors.lastname: Numazaki
mesh_terms: D000818:Animals; D002642:Chick Embryo; D003588:Cytopathogenic Effect, Viral; D004912:Erythrocytes / Q000382:microbiology; D006168:Guinea Pigs; D000882:Haplorhini; D009982:Gammainfluenzavirus / Q000302:isolation & purification*; D008545:Melanoma; D009975:Orthomyxoviridae / Q000302:isolation & purification*; D009976:Orthomyxoviridae Infections / Q000150:complications; D018184:Paramyxoviridae Infections / Q000150:complications; D018108:Respirovirus / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured; D014776:Virus Cultivation