Detection and typing of human papillomavirus DNA from cervical biopsies by the slot-blot hybridization method.
Cervical biopsies collected from 105 consecutive patients participating in a prospective follow-up study for cervical human papillomavirus (HPV) infections were tested for the presence of HPV 6, 11, 16 and 18 DNA by the slot-blot hybridization method. Detectable HPV types 6 and 11 could be distinguished in high stringency hybridization assay. The overall HPV-DNA detection rate was 35.2% in our group of patients. The slot-blot technique is more rapid than Southern-blotting procedure and well suited for analysis of multiple specimens.
David
F
F
Laboratoire d'Analyses Médicales du Dr Claude Lévy, Paris, France.
Levy
R
R
Lucotte
G
G
D001706:Biopsy; D002584:Cervix Uteri / Q000382:microbiology / Q000473:pathology*; D015344:DNA Probes, HPV / Q000032:analysis; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D014412:Tumor Virus Infections / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics; D002577:Uterine Cervical Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology
D015344:DNA Probes, HPV; D004279:DNA, Viral