Cloning of alpha- and beta-galactosidase genes from an extreme thermophile, Thermus strain T2, and their expression in Thermus thermophilus HB27.
The genes encoding thermostable alpha- and beta-galactosidases from an extremely thermophilic bacterium, Thermus strain T2, were cloned in Escherichia coli. The alpha-galactosidase gene was located just downstream from the beta-galactosidase gene. The genes were introduced into Thermus thermophilus HB27 with the aid of Thermus cryptic plasmid pTT8, and beta-galactosidases were expressed constitutively.
Koyama
Y
Y
Fermentation Research Institute, AIST, MITI, Tsukuba Science City, Ibaraki, Japan.
Okamoto
S
S
Furukawa
K
K
D003001:Cloning, Molecular; D005696:Galactosidases / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D010957:Plasmids; D013824:Thermus / Q000201:enzymology* / Q000235:genetics; D000519:alpha-Galactosidase / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics*
D005696:Galactosidases; D000519:alpha-Galactosidase; D001616:beta-Galactosidase