Ubiquitinated conjugates are found in preparations of several plant viruses.
Recently D.D. Dunigan, R.G. Dietzgen, J.E. Schoelz, and M. Zaitlin (Virology 165, 310-312, 1988) demonstrated that a small proportion of the subunits of tobacco mosaic virus particles were conjugated with the small protein ubiquitin. We have now detected ubiquitinated conjugates in immunoblots of virion preparations of several other plant viruses, using anti-human ubiquitin antiserum. Based on their polyacrylamide gel migrations, plant virus-associated ubiquitin-immunoreactive proteins were considered to be possible virus structural protein-ubiquitin conjugates of the following viruses: barley stripe mosaic, brome mosaic, cowpea mosaic (two proteins), cowpea severe mosaic (two proteins), and satellite panicum mosaic. Ubiquitinated conjugates were not detected in immunoblots of preparations of cucumber mosaic virus and Cymbidium mosaic virus. The significance of ubiquitinated viral proteins remains to be determined.
Hazelwood
D
D
Department of Plant Pathology, Cornell University, Ithaca, New York 14853.
Zaitlin
M
M
D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D008970:Molecular Weight; D009029:Mosaic Viruses / Q000032:analysis*; D011485:Protein Binding; D013045:Species Specificity; D014452:Ubiquitins / Q000302:isolation & purification*; D014764:Viral Proteins / Q000302:isolation & purification*; D014771:Virion / Q000032:analysis
D014452:Ubiquitins; D014764:Viral Proteins