Whole-mount preparations of mouse lens epithelium for the fluorescent cytological study of actin.
A technique is given for the preparation of a sheet of epithelial cells from the capsule of the crystalline lens. A new method is described for fixation and staining with fluorescent phalloidin or actin antibody in order to localize the actin cytoskeleton in this tissue. Optical section of the preparation resolves such actin features as apical polygonal arrays, sequestered actin bundles, perinuclear actin aggregates, observed here for the first time, and filamentous networks in the basal region of the cell. This method is superior to previous ones in its ability to preserve actin-abundant sectors distinctively.
Liou
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Department of Anatomy, Medical College, National Cheng Kung University, Tainan, Taiwan, Republic of China.
D000199:Actins / Q000032:analysis* / Q000276:immunology; D000818:Animals; D003599:Cytoskeleton / Q000032:analysis*; D004847:Epithelial Cells; D004848:Epithelium / Q000032:analysis; D005455:Fluorescent Antibody Technique; D007908:Lens, Crystalline / Q000032:analysis / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D010590:Phalloidine
D000199:Actins; D010590:Phalloidine