Cloning and analysis of rfb gene synthesizing the mannan 0 side chain of Escherichia coli 09 lipopolysaccharide.
The rfb gene encoding the proteins responsible for the synthesis of the repeating units (O side-chain) of Escherichia coli 09 lipopolysaccharide was cloned into a conjugative plasmid RP4::miniMu and was expressed in E. coli K-12.
Kido
N
N
Department of Bacteriology, Nagoya University School of Medicine, Aichi, Japan.
Ohta
M
M
Ito
H
H
Iida
K
K
Arakawa
Y
Y
Komatsu
T
T
Jann
K
K
Kato
N
N
D000942:Antigens, Bacterial / Q000235:genetics*; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D008050:Lipid A / Q000235:genetics / Q000276:immunology; D008070:Lipopolysaccharides / Q000096:biosynthesis / Q000235:genetics*; D008351:Mannans / Q000378:metabolism*; D010957:Plasmids; D015183:Restriction Mapping
D000942:Antigens, Bacterial; D003470:Culture Media; D004269:DNA, Bacterial; D008050:Lipid A; D008070:Lipopolysaccharides; D008351:Mannans; C017286:enterobacterial common antigen