Mutational analysis of cis elements involved in E2 modulation of human papillomavirus type 16 P97 and type 18 P105 promoters.
cis-Acting elements involved in E2 modulation of human papillomavirus type 16 (HPV-16) P97 promoter activity and HPV-18 P105 promoter activity were examined. In transfected primary human keratinocytes, each promoter had a basal activity that could be repressed by the bovine papillomavirus type 1 E2 gene product. Mutational analysis of the E2-binding sites in the long control region upstream of each promoter revealed that E2 repression was mediated through the E2-binding sites proximal to each promoter. In the context of a mutated E2-binding site at the promoter proximal position, the HPV-16 P97 and HPV-18 P105 promoters could be transactivated by E2. E2-mediated repression of HPV-18 P105 promoter activity was shown to be a transcriptional effect. The interaction of E2 with promoter-proximal E2-binding sites is likely to be important for the controlled expression of viral genes transcribed from the HPV-16 P97 promoter and the HPV-18 P105 promoter in infected human genital epithelial cells.
Romanczuk
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Laboratory of Tumor Virus Biology, National Cancer Institute, Bethesda, Maryland 20892.
Thierry
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F
Howley
P M
PM
D001483:Base Sequence; D002478:Cells, Cultured; D002874:Chromosome Mapping; D004274:DNA, Recombinant / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D027383:Papillomaviridae / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014162:Transfection
D004274:DNA, Recombinant; D015345:Oligonucleotide Probes