The major surface glycoprotein (GP63) is present in both life stages of Leishmania.
Leishmania exist as extracellular promastigotes which multiply in the gut of the sandfly insect vector and as intracellular amastigotes which divide in the phagolysosome of mononuclear phagocytic cells of the mammalian host. Promastigotes express a major surface glycoprotein of 63 kDa, referred to as GP63. The expression of GP63 in both Leishmania life stages was studied using rabbit antibodies against native GP63 as well as rabbit antibodies against recombinant GP63 that was synthesized in an Escherichia coli expression system. Immunofluorescence staining detected GP63 in intracellular amastigotes contained within a macrophage cell line and within freshly isolated lesion amastigotes. Western blot analysis using anti-recombinant GP63 antibodies also demonstrated that amastigotes synthesize GP63 which may undergo differential post-translational processing as compared to promastigote GP63.
Frommel
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TO
Department of Medical Genetics, University of British Columbia, Vancouver, Canada.
Button
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LL
Fujikura
Y
Y
McMaster
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WR
D000818:Animals; D000953:Antigens, Protozoan; D015153:Blotting, Western; D005455:Fluorescent Antibody Technique; D007891:Leishmania / Q000254:growth & development* / Q000378:metabolism; D007894:Leishmania mexicana / Q000254:growth & development; D007895:Leishmania tropica / Q000254:growth & development; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D008666:Metalloendopeptidases; D015800:Protozoan Proteins / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis
D000953:Antigens, Protozoan; D008562:Membrane Glycoproteins; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D008666:Metalloendopeptidases; C055146:glycoprotein gp63, Leishmania