Inhibition of pancreatic hepatocyte induction in hamsters treated sequentially with ethionine, a protein-free diet and then methionine by prior N-nitrosobis-(2-oxopropyl)amine administration.
A 100% yield of pancreatic hepatocytes was induced in pancreas tissues of female hamsters treated with twice-repeated sequential administrations of DL-ethionine (ethionine) together with a protein-free diet and then L-methionine (methionine) for 10 weeks. The cells were also found in 40% of hamsters receiving 20 mg/kg body weight of the pancreatic carcinogen, N-nitrosobis(2-oxopropyl)amine (BOP) given twice at the peak of pancreatic regeneration stimulated by methionine after ethionine-induced cell damage. However, BOP at doses of 30, 70, and 100 mg/kg body weight administered before the occurrence of pancreatic regeneration dose-dependently inhibited their appearance, with reduction of the yield to 40%, 25%, and 8.3% respectively, and BOP per se did not induce any development of pancreatic hepatocytes. Stein iodine staining revealed bile pigments in the induced hamster pancreatic eosinophilic cell populations.
Kitazawa
S
S
Department of Oncological Pathology, Nara Medical University, Japan.
Mizumoto
K
K
Amanuma
T
T
Tsutsumi
M
M
Maruyama
H
H
Konishi
Y
Y
D000230:Adenocarcinoma / Q000473:pathology / Q000503:physiopathology; D002759:Adenoma, Bile Duct / Q000473:pathology / Q000503:physiopathology; D000818:Animals; D002273:Carcinogens / Q000008:administration & dosage*; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D005001:Ethionine / Q000494:pharmacology*; D005260:Female; D007279:Injections, Subcutaneous; D008099:Liver / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D008647:Mesocricetus; D008715:Methionine / Q000494:pharmacology*; D009602:Nitrosamines / Q000008:administration & dosage*; D010179:Pancreas / Q000502:physiology*; D010190:Pancreatic Neoplasms / Q000473:pathology / Q000503:physiopathology; D011502:Protein-Energy Malnutrition / Q000503:physiopathology*
D002273:Carcinogens; D009602:Nitrosamines; C013622:nitrosobis(2-oxopropyl)amine; D008715:Methionine; D005001:Ethionine