title: The interaction between bovine herpesvirus type 1 and activated bovine T lymphocytes.
abstract: The interaction between activated bovine T lymphocytes (BTLs) and bovine herpesvirus type 1 (BHV-1) was investigated. BHV-1 infection of BTLs reduced the amplitude of recombinant bovine interleukin 2-induced proliferative responses. This decreased proliferation was caused by a virus-induced lymphocytolysis which was dependent on viable virus and was not inhibited by recombinant bovine interferon-alpha I1. Furthermore, lymphocytolysis was not associated with virus replication or with the synthesis of detectable levels of viral proteins. Electron microscopic examination of virus-infected cells revealed that lymphocytolysis was characterized by early nuclear disintegration resembling apoptosis. These observations suggest that activated T cells, localized at the site of BHV-1 infection, may be susceptible to virus-induced cytolysis.
authors.affiliation: Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
authors.forename: P J
authors.initials: PJ
authors.lastname: Griebel
authors.forename: H B
authors.initials: HB
authors.lastname: Ohmann
authors.forename: M J
authors.initials: MJ
authors.lastname: Lawman
authors.forename: L A
authors.initials: LA
authors.lastname: Babiuk
mesh_terms: D000818:Animals; D001345:Autoradiography; D002417:Cattle; D002470:Cell Survival; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D007242:Herpesvirus 1, Bovine / Q000502:physiology*; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000276:immunology / Q000382:microbiology*; D014764:Viral Proteins / Q000032:analysis; D014779:Virus Replication
chemical_list: D007376:Interleukin-2; D014764:Viral Proteins