A role of lyso-phosphatidylcholine in GM3-dependent inhibition of epidermal growth factor receptor autophosphorylation in A431 plasma membranes.
EGF-dependent receptor autophosphorylation (EDRA) in A431 plasma membrane was specifically stimulated by lysophospholipids having phosphorylcholine head group (e.g., lyso-phosphatidylcholine; lyso-PC) but not other lysophospholipids, in the absence of detergent. In contrast, GM3 specifically inhibited EDRA under the same experimental conditions in which lyso-PC stimulated EDRA. This GM3-dependent inhibition was more efficient in the absence (vs. presence) of a detergent (Triton X-100). These results indicate an essential role of lyso-PC in GM3-regulated EGF receptor functions.
Igarashi
Y
Y
Biomembrane Institute, Seattle, WA 98119.
Kitamura
K
K
Zhou
Q H
QH
Hakomori
S
S
D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003902:Detergents / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D066246:ErbB Receptors / Q000187:drug effects / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology; D005679:G(M3) Ganglioside / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D008954:Models, Biological; D017830:Octoxynol; D010743:Phospholipids / Q000494:pharmacology; D010766:Phosphorylation; D011092:Polyethylene Glycols / Q000494:pharmacology; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D013329:Structure-Activity Relationship
D003902:Detergents; D005230:Fatty Acids, Nonesterified; D005679:G(M3) Ganglioside; D008244:Lysophosphatidylcholines; D010743:Phospholipids; D011092:Polyethylene Glycols; D004815:Epidermal Growth Factor; D017830:Octoxynol; D066246:ErbB Receptors; D011505:Protein-Tyrosine Kinases