Characterization by T1-oligonucleotide fingerprinting of three strains of human hepatitis A virus isolated in Italy.
Three human hepatitis A virus strains, all of them isolated in Italy but one acquired abroad, were analyzed by T1-RNAase oligonucleotide mapping and by monoclonal antibody neutralization. The variation among their genomes according to T1-maps was calculated to be about 9%, thus confirming the poor genomic variation assessed by nucleotide sequencing (1-10%). However T1-maps of these Italian isolates were different from those reported in the literature (Weitz and Siegl, 1985). Neutralization by monoclonal antibody caused a reduction in titres of 2-25 log10. This genomic stability, if confirmed, is important with a view to a valuable vaccine.
Fiore
L
L
Department of Virology, Istituto Superiore di Sanità, Rome, Italy.
De Paolis
E
E
Novello
F
F
Divizia
M
M
Panà
A
A
D000328:Adult; D002648:Child; D002675:Child, Preschool; D004812:Epidemiologic Methods; D005260:Female; D014644:Genetic Variation; D006506:Hepatitis A / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*; D006507:Hepatovirus / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007558:Italy; D008297:Male; D009710:Nucleotide Mapping; D009841:Oligonucleotides / Q000032:analysis* / Q000235:genetics
D009841:Oligonucleotides