title: The Drosophila PROS-28.1 gene is a member of the proteasome gene family.
abstract: In the present communication, we report the identification of a new gene family which encodes the protein subunits of the proteasome. The proteasome is a high-Mr complex possessing proteolytic activity. Screening a Drosophila lambda gt11 cDNA expression library with the proteasome-specific antibody N19-28 we isolated a clone encoding the 28-kDa No. 1 proteasome protein subunit. In accordance with the nomenclature of proteasome subunits in Drosophila, the corresponding gene is designated PROS-28.1, and it encodes an mRNA of 1.1 kb with an open reading frame of 249 amino acids (aa). Genomic Southern-blot hybridization shows PROS-28.1 to be a member of a family of related genes. Analysis of the predicted aa sequence reveals a potential nuclear targeting signal, a potential site for tyrosine kinase and a potential cAMP/cGMP-dependent phosphorylation site. The aa sequence comparison of the products of PROS-28.1 and PROS-35 with the C2 proteasome subunit of rat shows a strong sequence similarity between the different proteasome subunits. The data suggest that at least a subset of the proteasome-encoding genes belongs to a family of related genes (PROS gene family) which may have evolved from a common ancestral PROS gene.
authors.affiliation: ZMBH/Molekulare Genetik, University of Heidelberg, F.R.G.
authors.forename: C
authors.initials: C
authors.lastname: Haass
authors.forename: B
authors.initials: B
authors.lastname: Pesold-Hurt
authors.forename: G
authors.initials: G
authors.lastname: Multhaup
authors.forename: K
authors.initials: K
authors.lastname: Beyreuther
authors.forename: P M
authors.initials: PM
authors.lastname: Kloetzel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D001483:Base Sequence; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000235:genetics* / Q000276:immunology; D004247:DNA / Q000032:analysis; D004330:Drosophila / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics* / Q000276:immunology; D005810:Multigene Family; D010766:Phosphorylation; D046988:Proteasome Endopeptidase Complex; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D012689:Sequence Homology, Nucleic Acid; D013329:Structure-Activity Relationship
chemical_list: D009097:Multienzyme Complexes; D012333:RNA, Messenger; D004247:DNA; D000242:Cyclic AMP; D011505:Protein-Tyrosine Kinases; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex; D006152:Cyclic GMP