title: Ectopic expression of c-jun leads to differentiation of P19 embryonal carcinoma cells.
abstract: The product of the c-jun proto-oncogene, a major component of the transcription factor AP1, has been implicated in both positive and negative transcriptional control as well as in transformation. The role of c-jun in early development and differentiation processes, however, is still largely unknown. In this paper we show that ectopic expression of exogenous c-jun sequences leads to differentiation and loss of the transformed phenotype of P19 embryonal carcinoma (EC) cells. The mixed populations of endoderm- and mesoderm-like cells that were obtained after the introduction of c-jun morphologically and biochemically resembled P19 cells differentiated in monolayer by retinoic acid (RA). Furthermore, we provide evidence that direct effects of the c-jun gene product on transcription of the retinoic acid receptor beta (RAR beta) gene may be responsible for the differentiation-promoting potential of c-jun.
authors.affiliation: Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.
authors.forename: R P
authors.initials: RP
authors.lastname: de Groot
authors.forename: F A
authors.initials: FA
authors.lastname: Kruyt
authors.forename: P T
authors.initials: PT
authors.lastname: van der Saag
authors.forename: W
authors.initials: W
authors.lastname: Kruijer
mesh_terms: D002352:Carrier Proteins / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D004247:DNA / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D054278:Embryonal Carcinoma Stem Cells; D006801:Humans; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000187:drug effects; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics; D016755:Proto-Oncogene Proteins c-jun; D011519:Proto-Oncogenes; D018168:Receptors, Retinoic Acid; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D014162:Transfection; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D016755:Proto-Oncogene Proteins c-jun; D018168:Receptors, Retinoic Acid; D014157:Transcription Factors; D014212:Tretinoin; D004247:DNA