title: The major transcriptional regulatory protein of herpes simplex virus type 1 includes a protease resistant DNA binding domain.
abstract: Herpes simplex virus type 1 expresses five immediate-early (IE) polypeptides. In the absence of functional Vmw175 (the product of IE gene 3) activation of transcription of later classes of viral genes and repression of IE gene expression does not occur. The recognition of specific DNA sequences by Vmw175 requires, as determined by sensitivity to mutation, a part of the protein highly conserved in the corresponding proteins of related herpes viruses. However, mutations in other parts of the protein can also disrupt specific DNA binding. This paper shows that the DNA binding domain of Vmw175 can be liberated as a functional unit by digestion with proteinase K. Analysis of mutant Vmw175 proteins showed that the proteinase K resistant domain has an amino terminus between amino acid residues 229 and 292, while its carboxy terminus is between residues 495 and 518. Mutations outside this region which affect DNA binding by the intact protein do not eliminate binding of the proteinase K resistant domain. This implies that direct DNA binding by Vmw175 involves a linear subsection of the polypeptide, and that mutations in other parts of the polypeptide which affect DNA binding of the whole protein do so by indirect means.
authors.affiliation: MRC Virology Unit, Institute of Virology, Glasgow, UK.
authors.forename: R D
authors.initials: RD
authors.lastname: Everett
authors.forename: T
authors.initials: T
authors.lastname: Paterson
authors.forename: M
authors.initials: M
authors.lastname: Elliott
mesh_terms: D004279:DNA, Viral / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D019286:Endopeptidase K; D015967:Gene Expression Regulation, Viral; D006367:HeLa Cells; D006801:Humans; D009154:Mutation; D012697:Serine Endopeptidases / Q000378:metabolism; D018139:Simplexvirus / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; D014157:Transcription Factors; D012697:Serine Endopeptidases; D019286:Endopeptidase K