title: An insertion vector for the analysis of gene expression during herpes simplex virus infection.
abstract: A herpes simplex virus type 1 (HSV-1) insertion vector, pGal8, was designed for analysis of herpesvirus promoters during virus infection. This vector contains a multiple cloning site (MCS) positioned at the 5' end of the lacZ gene for the insertion of promoter sequences. The MCS and lacZ are flanked by sequences from the HSV-1 thymidine kinase encoding gene (tk) to direct homologous recombination into the tk locus of the viral genome. The utility of this vector is demonstrated by construction and comparison of recombinant viruses that express lacZ from the promoters of the genes encoding glycoprotein C, glycoprotein H and glycoprotein E.
authors.affiliation: Department of Microbiology, University of Tennessee, Knoxville 37996.
authors.forename: J P
authors.initials: JP
authors.lastname: Weir
authors.forename: K R
authors.initials: KR
authors.lastname: Steffy
authors.forename: M
authors.initials: M
authors.lastname: Sethna
mesh_terms: D001483:Base Sequence; D002472:Cell Transformation, Viral; D004251:DNA Transposable Elements; D015870:Gene Expression; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D018139:Simplexvirus / Q000235:genetics*; D013728:Terminator Regions, Genetic; D013937:Thymidine Kinase / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; D013937:Thymidine Kinase; D001616:beta-Galactosidase