Effect of protein kinase modulators on the induction of morphological differentiation of pheochromocytoma PC12 cells by nerve and fibroblast growth factors.
The effect of protein kinase modulators on the ability of nerve and fibroblast growth factors to induce neurite outgrowth in pheochromocytoma PC12 cells was studied. The protein kinase inhibitor H7 increased the neurite-stimulating capacity of these factors. The effect of H7 was observed within 1 h and was dose-dependent. HA 1004, an inhibitor of cAMP- and cGMP-dependent protein kinases, did not affect the neurite-stimulating activity of NGF. Substances inhibiting protein kinase C, ganglioside GT1b and quercetin, acted in a similar way whereas sphingosine had an opposite effect.
Posypanova
G A
GA
Research Center of Molecular Diagnostics, USSR Ministry of Health, Moscow.
Severin
S E
SE
Kondratyev
A D
AD
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D001369:Axons / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects; D000242:Cyclic AMP / Q000494:pharmacology; D006152:Cyclic GMP / Q000494:pharmacology; D004357:Drug Synergism; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D007546:Isoquinolines / Q000494:pharmacology; D009414:Nerve Growth Factors / Q000494:pharmacology*; D010673:Pheochromocytoma / Q000473:pathology*; D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D013449:Sulfonamides; D014407:Tumor Cells, Cultured
D007546:Isoquinolines; D009414:Nerve Growth Factors; D010879:Piperazines; D047428:Protein Kinase Inhibitors; D013449:Sulfonamides; D005346:Fibroblast Growth Factors; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C043204:N-(2-guanidinoethyl)-5-isoquinolinesulfonamide; D000242:Cyclic AMP; D011494:Protein Kinases; D011493:Protein Kinase C; D006152:Cyclic GMP