Two-colour immunofluorescence marker study of pleomorphic adenomas.
Extensive use of two-colour immunofluorescence staining for various cell markers in pleomorphic adenoma, revealed three consistent phenotypic features: (1) keratin polypeptide No. 14, which was virtually restricted to myoepithelial cells (MEC) in normal salivary glands, appeared in a large fraction of the tumour cells, suggesting that the principal neoplastic element is derived from MEC or their immediate precursors; (2) a complex co-expression pattern of various cell markers was found, with extensive concurrence of keratin and vimentin in strands of MEC-like and myxoid tumour cells, probably reflecting different degrees of tumour cell differentiation; and (3) two phenotypically distinctive dendritic cell populations were identified, one consisting of keratin positive tumour cells and the other of HLA-DR positive but keratin negative stromal cells. The significance of these findings with regard to the histogenesis and complex morphology of pleomorphic adenoma is discussed.
Thrane
P S
PS
Laboratory for Immunohistochemistry and Immunopathology (LIIPAT), Medical Faculty, National Hospital, Rikshospitalet, Oslo, Norway.
Roop
D R
DR
Sollid
L M
LM
Huitfeldt
H S
HS
Brandtzaeg
P
P
D008949:Adenoma, Pleomorphic / Q000032:analysis*; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D005260:Female; D005455:Fluorescent Antibody Technique; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007381:Intermediate Filament Proteins / Q000032:analysis; D008297:Male; D008875:Middle Aged; D010307:Parotid Neoplasms / Q000032:analysis*; D009418:S100 Proteins / Q000032:analysis; D014841:von Willebrand Factor / Q000032:analysis
D014408:Biomarkers, Tumor; D006684:HLA-DR Antigens; D007381:Intermediate Filament Proteins; D009418:S100 Proteins; D014841:von Willebrand Factor