Hamster cell line suitable for transfection assay of transforming genes.
We established a subclone, SHOK, from the GHE-L cell line, an immortal line derived from a primary culture of Syrian hamster embryo cells, as a recipient cell line useful for the detection of oncogenes by transfection. SHOK cells were almost as susceptible as NIH 3T3 cells to focus formation by many oncogenes, including v-raf, v-Ha-ras, v-Ki-ras, or activated c-Ha-ras. The susceptibility of SHOK to focus formation was higher than that of NIH 3T3 for v-mos but was lower for v-fps, v-fgr, v-src, v-sis, and v-abl. When DNAs extracted from 27 human and murine tumors were tested for focus formation, 5 DNAs were positive in NIH 3T3 cells, whereas 9 were positive in SHOK cells at the primary transfection. Using SHOK cells as recipients of tumor cellular DNA, we isolated another oncogene and a c-Ki-ras2 gene mutated at codon 146 that were difficult to detect in NIH 3T3 cells. SHOK cells have a low rate of spontaneous transformation, produce easily distinguishable foci, and maintain a stable karyotype in transformed cells. In addition to being useful for the screening of human tumor DNAs, SHOK cells will be useful for the isolation of oncogenes from murine tumors because of their hamster origin.
Higashi
T
T
Department of Oncogene Research, Osaka University, Japan.
Sasai
H
H
Suzuki
F
F
Miyoshi
J
J
Ohuchi
T
T
Takai
S
S
Mori
T
T
Kakunaga
T
T
D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D003001:Cloning, Molecular; D006224:Cricetinae; D004273:DNA, Neoplasm / Q000235:genetics*; D011905:Genes, ras; D006801:Humans; D008647:Mesocricetus; D051379:Mice; D009857:Oncogenes; D010957:Plasmids; D014162:Transfection
D004273:DNA, Neoplasm