title: Isolation and characterization of the gene for a yeast mitochondrial import receptor.
abstract: We have previously identified an integral membrane protein (p32) from Saccharomyces cerevisiae as a receptor for protein import into mitochondria, and have localized it to the mitochondrial outer membrane at contact sites. Here we report isolation of the corresponding mitochondrial import receptor gene, termed MIR1. The deduced amino-acid sequence of p32 shows roughly 40% identity with proteins of bovine heart and rat liver that have been suggested to be mitochondrial phosphate carriers. Haploid cells carrying a disrupted MIR1 allele were unable to grow on a non-fermentable carbon source but grew in media containing glucose, indicating that the MIR1 protein is essential for mitochondrial function. Compared with wild type, amounts of some mitochondrial proteins were markedly reduced in cells containing a disrupted MIR1 allele, whereas levels of others were unchanged. This indicates that yeast contains more than one pathway for protein import into mitochondria.
authors.affiliation: Howard Hughes Medical Institute, Rockefeller University, New York, New York 10021.
authors.forename: H
authors.initials: H
authors.lastname: Murakami
authors.forename: G
authors.initials: G
authors.lastname: Blobel
authors.forename: D
authors.initials: D
authors.lastname: Pain
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D003001:Cloning, Molecular; D004272:DNA, Mitochondrial / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D007526:Isoelectric Point; D008565:Membrane Proteins / Q000235:genetics*; D008928:Mitochondria / Q000502:physiology*; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D012331:RNA, Fungal / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial; D005656:Fungal Proteins; D008565:Membrane Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger; D011956:Receptors, Cell Surface