A modified and improved assay for sperm amidase activity.
An assay for sperm amidase activity has been modified so that many samples can be measured rapidly, making it more suitable for use in a clinical setting. The modified assay gave the same results (P greater than 0.05) as its lengthier parent assay (Kennedy et al, 1989). The measured enzyme activity was proportional to the number of sperm assayed over the range tested (3.5 x 10(5) to 2.1 x 10(6) sperm). The within-assay coefficient of variation was 7.8 +/- 1.8% (average +/- SE, n = 12), and the between-assay coefficient of variation was 7.2 +/- 1.2% (n = 3). The sensitivity was 7.6 microIU/well.
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Department of Obstetrics and Gynecology, University of California, Davis 95616.
D000176:Acrosin / Q000032:analysis*; D000581:Amidohydrolases / Q000032:analysis*; D001120:Arginine / Q000031:analogs & derivatives; D001586:Benzoylarginine Nitroanilide; D002863:Chromogenic Compounds; D006801:Humans; D008297:Male; D008832:Microchemistry; D013053:Spectrophotometry; D013094:Spermatozoa / Q000201:enzymology*
D002863:Chromogenic Compounds; D001586:Benzoylarginine Nitroanilide; D001120:Arginine; D000176:Acrosin; D000581:Amidohydrolases; C019665:amidase