Preferential usage of the V beta 8 gene family by CD4-CD8-T cell lines derived from spleen.
Receptors encoded by the V beta 8 gene family and identified by the F23.1 antibody are commonly expressed amongst the CD4-CD8-T cell lines isolated from spleen cells infected in vitro with the RadLV retrovirus. All but one out of 12 cell lines showed between 50 and 85% F23.1+ cells in the uncloned cell population which is noticably higher than the approximately 13% level amongst the Ig- normal spleen cell population. There was a high frequency (approximately 50%) of F23.1+ clones from five of these cell lines. The frequency of F23.1 binding cells in the Ig-, CD4/CD8-depleted spleen population is only 0.2%, which gives a precursor frequency in spleen of less than 0.002%. This reflects selective isolation of CD4-CD8- alpha beta+ cells which express V beta 8 gene products by this culture scheme. The requirement for RadLV in induction of these cell lines has been established, suggesting that this retrovirus may selectively stimulate CD4-CD8-F23.1+ T cells. These cells may represent an autoimmune subset present in peripheral lymphoid tissue.
O'Neill
H C
HC
Developmental Haematology Group, John Curtin School of Medical Research, Australian National University, Canberra ACT.
D000818:Animals; D000911:Antibodies, Monoclonal; D002461:Cell Line, Transformed; D005786:Gene Expression Regulation; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006133:Growth Substances / Q000502:physiology; D009052:Leukemia Virus, Murine; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D005810:Multigene Family; D011948:Receptors, Antigen, T-Cell / Q000096:biosynthesis* / Q000235:genetics; D016693:Receptors, Antigen, T-Cell, alpha-beta; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006133:Growth Substances; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta