The endomembrane system of cholinergic and non-cholinergic neurons in the medial septal nucleus and vertical limb of the diagonal band of Broca: a cytochemical and immunocytochemical study.
Coronal vibratome sections of the rostral part of the medial septum (MS) and vertical limb of the diagonal band of Broca (VDB) nuclei were studied by an immunocytochemical technique using a monoclonal antibody against choline acetyltransferase (ChAT) and a double histochemical method for detection of acid phosphatase (AcPase) and nucleoside diphosphatase (NDPase) activity. The electron microscopic morphology of ChAT-immunoreactive and non-immunoreactive neurons was compared with similar neurons showing both AcPase and NDPase activity. ChAT-labeled and non-labeled neurons were well differentiated by the organization of the endomembrane system and especially by the structure of the rough endoplasmic reticulum (RER) and associated lamellar bodies. These results support the theory that the peculiar ultrastructure of the lamellar bodies in each neuron is related to the pattern of organization of the endomembrane system and its function. The significance of the lamellar bodies is discussed, and the data of the present work, together with findings described by other investigators. These data suggest that these bodies are predominant in efferent projection neurons in the basal forebrain nuclei.
Palacios
G
G
Department of Cellular Biology and Physiology, Faculty of Medicine, Autonomous University, Barcelona, Spain.
D017766:Acid Anhydride Hydrolases; D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D002799:Cholinergic Fibers / Q000201:enzymology / Q000648:ultrastructure*; D005625:Frontal Lobe / Q000166:cytology / Q000201:enzymology / Q000648:ultrastructure*; D006651:Histocytochemistry; D007150:Immunohistochemistry; D007425:Intracellular Membranes / Q000201:enzymology / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D009474:Neurons / Q000201:enzymology / Q000648:ultrastructure*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D051381:Rats; D012686:Septal Nuclei / Q000166:cytology / Q000201:enzymology / Q000648:ultrastructure*
D002795:Choline O-Acetyltransferase; D000135:Acid Phosphatase; D010744:Phosphoric Monoester Hydrolases; D017766:Acid Anhydride Hydrolases; C023837:nucleoside-diphosphatase