Using different sorbents for the concentration of enteroviruses.
Comparative investigations were carried out to evaluate the efficiency of concentration (EC) of enteroviruses (poliovirus type 1 and simian rotavirus SA-11) using macroporous glass (brands MPG-1000 VGKh, USSR, and SO1, Czechoslovakia) and membrane filters (MF): (nitrocellulose PNTs 0.45, USSR, Millipore HAWP 0.45, USA, Synpor 0.45, Czechoslovakia as well as polycapromide MF Pall 0.2, FRG, and FMPA 0.55, USSR). To assess the sorption properties of filters, poliovirus preparations and rabbit serum gamma-globulin were labelled with radioactive isotopes. Nitrocellulose filters (Millipore and PNTs) proved to be superior in providing for 64-90% virus sorption and 20-24% protein sorption. Elution experiments using solutions of different chemical nature--protein solutions (triptosophosphate broth and beef extract), amino acid mixture (glycine + arginine), chaotropic salt (sodium trichloroacetate mixed with lysine)--showed that protein solutions better eluted rotavirus SA-11 from nitrocellulose filters and macroporous glass (MPG). The utilization of nitrocellulose filters and MPG as sorbents enables a 10-40-fold concentration of enteroviruses depending on the chosen eluent. Comparisons of EC values for rotavirus SA-11 obtained using porous glass MPS-1000 VGKh and SO1 indicated that MPS-1000 VGKh was superior both with respect to recovery efficiency (R) and concentration factor (CF).
Bayeva LYe
Institute of Poliomyelitis and Viral Encephalitis, USSR Academy of Medical Sciences, Moscow.
Shirman
G A
GA
Ginevskaya
V A
VA
Kucharská
Z
Z
Dotlacilová
L
L
Danes
L
L
Kazantseva
V A
VA
Drozdov
S G
SG
D000327:Adsorption; D004770:Enterovirus / Q000032:analysis*; D005374:Filtration / Q000379:methods; D005898:Glass; D007124:Immunoenzyme Techniques; D012401:Rotavirus / Q000032:analysis; D014871:Water Microbiology