Soybean lipoxygenase-1 is not a quinoprotein.
Soybean lipoxygenase-1 was reinvestigated with respect to its quinoprotein nature. It has been reported previously that soybean lipoxygenase-1 contains pyrroloquinoline quinone as the organic cofactor. Because spectroscopic data were found to be inconsistent with the evidence presented in [1], we sought to reproduce the published data by carefully following the procedures described in [1] and supplementing them with new analytical results. The combined data lead us to conclude that soybean lipoxygenase-1 is not a quinoprotein.
Veldink
G A
GA
Bijvoet Center for Biomolecular Research, Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.
Boelens
H
H
Maccarrone
M
M
van der Lecq
F
F
Vliegenthart
J F
JF
Paz
M A
MA
Fluckiger
R
R
Gallop
P M
PM
D003067:Coenzymes / Q000032:analysis; D005562:Formazans / Q000032:analysis; D000441:Hexanols; D008084:Lipoxygenase / Q000737:chemistry*; D045542:PQQ Cofactor; D010659:Phenylhydrazines; D015363:Quinolones / Q000032:analysis*; D013025:Glycine max / Q000201:enzymology*; D013053:Spectrophotometry
D003067:Coenzymes; D005562:Formazans; D000441:Hexanols; D010659:Phenylhydrazines; D015363:Quinolones; C030299:phenylhydrazine; C004787:2,4-dinitrophenylhydrazine; D045542:PQQ Cofactor; D008084:Lipoxygenase