Ecdysteroids increase the yield of recombinant protein produced in baculovirus insect cell expression system.
Spodoptera frugiperda cells are the hosts of wild type and recombinant virus in the baculovirus insect cell expression system. The expression of the foreign gene could be enhanced by the addition of ecdysteroids and increased amount of recombinant protein was secreted into the medium. The time and concentration dependence of this effect was followed in the case of 20-hydroxyecdysone-, makisterone and ecdysone. 20-hydroxyecdysone proved to be the most efficient, producing a three fold increase in the level of recombinant protein secreted into the medium, as it was measured by ELISA. This effect was also confirmed by tracing the L-(35S)methionine incorporation into the gene product. Makisterone was also effective in stimulation, while ecdysone proved to be ineffective.
Sárvári
M
M
Institute of Enzymology, Hungarian Academy of Sciences, Budapest.
Csikós
G
G
Sass
M
M
Gál
P
P
Schumaker
V N
VN
Závodszky
P
P
D000818:Animals; D002460:Cell Line; D015923:Complement C1r / Q000096:biosynthesis / Q000235:genetics*; D004440:Ecdysone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004441:Ecdysterone / Q000494:pharmacology*; D015870:Gene Expression; D006801:Humans; D007304:Insect Viruses / Q000187:drug effects / Q000235:genetics*; D007700:Kinetics; D009036:Moths; D011994:Recombinant Proteins / Q000096:biosynthesis
D011994:Recombinant Proteins; C008310:makisterone; D004440:Ecdysone; D004441:Ecdysterone; D015923:Complement C1r