Glucose-silica, an improved medium for high-pressure gel filtration chromatography.
Glucose was covalently coupled to aminopropyl derivatized silica of various pore sizes. The reaction most likely involved Schiff's base formation between the aldehyde of glucose and the amine on the silica which was then reduced with NaCNBH3. This glucose-silica behaved as a nearly ideal molecular sieving support for the high-pressure gel filtration chromatography of proteins and was superior in performance to many currently available supports.
Lee
H G
HG
Department of Biochemistry, School of Medicine, University of Tennessee, Memphis 38138.
Jarrett
H W
HW
D002147:Calmodulin / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002627:Chemistry, Physical; D002850:Chromatography, Gel / Q000379:methods*; D005947:Glucose; D006863:Hydrogen-Ion Concentration; D007075:Immunoglobulin M / Q000032:analysis; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis; D012822:Silicon Dioxide
D002147:Calmodulin; D007075:Immunoglobulin M; D011506:Proteins; D012822:Silicon Dioxide; D005947:Glucose