Production of human papillomavirus type 16 virions in a keratinocyte cell line.
Human papillomavirus type 16 (HPV-16) is strongly associated with carcinoma of the cervix, but the complete life cycle of the virus cannot be studied because no experimental system is available in which HPV-16 progeny are produced, and there is currently no source of HPV-16 virus particles. Most cell lines that harbor HPV-16 DNA contain the viral genome as integrated or concatenated DNA in which open reading frames are disrupted or deleted, but a human cervical keratinocyte cell line has been described which maintains HPV-16 DNA in monomeric episomal form (M.A. Stanley, H.M. Brown, M.W. Appleby, and A.C. Minson, Int. J. Cancer 43:672-676, 1989). This cell line was induced to form a stratified differentiating epithelium by grafting onto nude mice. Long-term grafts displayed the histological features of a low-grade cervical dysplasia, and terminally differentiated cells contained amplified levels of HPV-16 DNA, virus capsid antigen, and virus particles. This experimental system appears to permit the completion of the HPV-16 life cycle in virus-containing keratinocytes.
Sterling
J
J
Department of Pathology, University of Cambridge, United Kingdom.
Stanley
M
M
Gatward
G
G
Minson
T
T
D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D015603:Keratinocytes; D008854:Microscopy, Electron; D016366:Open Reading Frames; D027383:Papillomaviridae / Q000235:genetics* / Q000648:ultrastructure; D014771:Virion / Q000235:genetics* / Q000502:physiology / Q000648:ultrastructure; D014779:Virus Replication
D004279:DNA, Viral