Immunophenotyping of childhood astrocytomas with a library of monoclonal antibodies.
Immunophenotype (IP) analysis of 14 childhood glial tumors was performed with a library of 16 monoclonal antibodies (MAbs) using biotin-streptavidin-alkaline phosphatase immunohistochemical detection technique. Presence of glial or neuronal differentiated cells within the tumors was evaluated with MAbs against cell-lineage-specific markers: high-, medium- and low-molecular-weight neurofilament protein (NFP) and glial fibrillary acidic protein (GFAP). Intense expression of GFAP was demonstrated in 14/14 astrocytomas. The three NFs were detected in 10-50% of the cells in 6/14 cases. The pan-neuro-ectodermal antigen defined by MAb UJ 13/A was present in 7/14 astrocytomas on more than 10% of the cells. Thy-1 was expressed in 14/14 tumors on more than 50% of their cells. The GQ ganglioside antigen detected by MAB A2B5, was found in 12/14 tumors. Shared antigens exist among morphologically benign and malignant glial tumor cells and leukocytes detectable with the following four MAbs: Thy-1, PI 153/3, UJ 308 and anti-HLe, common leukocyte antigen (CLA). CLA-expressing cells were demonstrated in 8/12 astrocytomas, and in 4/12 cases more than 90% of the cells were positive. We have shown that cells within childhood astrocytomas can express neuronal IP. The most common expressed phenotype for glial tumors was: GFAP+, Thy-1+, A2B5+, UJ 167.11+, UJ 223.8+, NF (H,M)+, UJ 13/A+, UJ 127.11-, and NF (L)-.
Bodey
B
B
Childrens Hospital, Los Angeles, CA 90054.
Zeltzer
P M
PM
Saldivar
V
V
Kemshead
J
J
D000293:Adolescent; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions / Q000276:immunology; D001254:Astrocytoma / Q000145:classification* / Q000276:immunology; D001932:Brain Neoplasms / Q000145:classification* / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D005909:Glioblastoma / Q000145:classification* / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007223:Infant; D010641:Phenotype
D000911:Antibodies, Monoclonal