Dual effects of dopamine in rat adrenal glomerulosa cells.
The effects of dopamine (DA) on cAMP production and aldosterone secretion were compared in freshly isolated cells and in primary cultures of rat adrenal glomerulosa cells. Under isolated conditions, glomerulosa cells exhibited dopamine receptors from DA-1 and DA-2 subclass, whereas in cultured conditions, where cells are very sensitive to their known stimuli, cells only exhibited dopamine receptors from the DA-1 subclass. Moreover, unlike freshly isolated cells, dopamine stimulated both cAMP production and aldosterone secretion in 3-day cultured preparations. These effects were receptor specific since they were completely suppressed by Scherring 23390 (a specific DA-1 antagonist) and were unaffected by a beta-adrenergic antagonist. As in vivo rat adrenal cortex contains DA, we discuss a possible involvement of this neurotransmitter in the regulation of aldosterone secretion.
Gallo-Payet
N
N
Department of Medicine, Faculty of Medicine, University of Sherbrooke, Quebec, Canada.
Chouinard
L
L
Balestre
M N
MN
Guillon
G
G
D000450:Aldosterone / Q000378:metabolism*; D000818:Animals; D001552:Benzazepines / Q000494:pharmacology; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D004298:Dopamine / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004837:Epinephrine / Q000494:pharmacology; D005260:Female; D051381:Rats; D011954:Receptors, Dopamine / Q000145:classification / Q000187:drug effects / Q000378:metabolism*; D015384:Zona Glomerulosa / Q000187:drug effects / Q000378:metabolism*
D001552:Benzazepines; D011954:Receptors, Dopamine; D000450:Aldosterone; D000242:Cyclic AMP; D004298:Dopamine; D004837:Epinephrine