Interferon-alpha modulates the plasma membrane-cytoskeletal complex of human lymphoblastoid cells sensitive to the antiproliferative action of interferon-alpha.
Interferon (IFN)-induced structural changes in the plasma membrane-cytoskeletal complex were investigated in human lymphoblastoid cell lines that differed in sensitivity to the antiproliferative action of IFN-alpha. These cell lines have structurally indistinguishable high-affinity IFN-alpha receptors. By using electron spin resonance techniques, we determined the effect of IFN-alpha on fluidity of the plasma membrane lipid bilayer. By using the ability of cells to redistribute fluorescent anti-immunoglobulin (Ig) into caps at the cell surface, we investigated the effect of IFN-alpha on the mobility of the cell surface. IFN-alpha decreases the fluidity of the plasma membrane lipid bilayer of lymphoblastoid cells sensitive to the antiproliferative action of IFN-alpha. Similarly, IFN-alpha inhibits the anti-Ig-induced redistribution of surface immunoglobulins (capping) of IFN-sensitive cells. These effects of IFN-alpha occur within the first few hours after IFN-alpha addition to cells. However, IFN-alpha had no effect on membrane fluidity or anti-Ig-induced capping of lymphoblastoid cells resistant to the antiproliferative action of IFN-alpha.
Pfeffer
L M
LM
Rockefeller University, New York, NY 10021.
Landsberger
F R
FR
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D003599:Cytoskeleton / Q000187:drug effects*; D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D008051:Lipid Bilayers; D008214:Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure; D011947:Receptors, Antigen, B-Cell / Q000187:drug effects
D007370:Interferon Type I; D008051:Lipid Bilayers; D011947:Receptors, Antigen, B-Cell