title: Comparison of in situ hybridization and immunohistochemistry for detection of cytomegalovirus and herpes simplex virus.
abstract: In situ hybridization (ISH) and immunohistochemistry (IHC) were compared for detection of cytomegalovirus (CMV) and herpes simplex virus (HSV) in routinely processed tissue. Fifty-four formalin-fixed paraffin-embedded tissue samples infected with CMV (36 tissues) or HSV (18 tissues) from 30 autopsies were studied. All tissues had either positive viral cultures (38 of 54) or characteristic viral inclusions on hematoxylin and eosin examination (39 of 54). The tissues examined included lung (28), liver (nine), kidney (five), heart (three), adrenal (two), spleen (two), and thymus, pancreas, appendix, esophagus, and duodenum (one each). Studies by ISH were performed with two detection systems, using biotinylated probes to CMV and HSV (Enzo Biochem, New York, NY). Using ISH with an alkaline phosphatase detection system, infected cells were detected in 33 of 54 tissues (CMV: 23 of 36, HSV: 10 of 18). Using ISH with a peroxidase detection system, infected cells were identified in 30 of 54 tissues (CMV: 22 of 36, HSV: eight of 18). With IHC, antibodies to CMV and HSV stained the infected cells in 34 of 54 tissues (CMV: 24 of 36, HSV: 10 of 18). All infections detected with ISH were also detected with IHC. We conclude that these techniques for ISH and IHC are equally effective for detecting CMV and HSV in paraffin sections. The results of both techniques correlate better with viral inclusions than with culture results. The ISH stains are more difficult to prepare and in some cases are more difficult to interpret. Therefore, IHC may be preferable to ISH for detecting CMV and HSV in routine diagnostic work.
authors.affiliation: Department of Laboratory Medicine and Pathology, University of Minnesota Hospital, Minneapolis 55455.
authors.forename: J G
authors.initials: JG
authors.lastname: Strickler
authors.forename: J C
authors.initials: JC
authors.lastname: Manivel
authors.forename: C M
authors.initials: CM
authors.lastname: Copenhaver
authors.forename: V L
authors.initials: VL
authors.lastname: Kubic
mesh_terms: D003587:Cytomegalovirus / Q000302:isolation & purification*; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D009693:Nucleic Acid Hybridization; D015341:Nucleic Acid Probes; D018139:Simplexvirus / Q000302:isolation & purification*
chemical_list: D015341:Nucleic Acid Probes