Serotype analysis of group A human rotavirus related to acute gastroenteritis in winter in Gifu city.
A total of 348 fecal specimens collected from children with acute gastroenteritis in Gifu city over three consecutive winter seasons (from November 1986 to March 1989) were examined for group A human rotavirus (HRV) by either a commercial test kit or a sandwich enzyme-linked immunosorbent assay (sandwich ELISA). One hundred twelve of the 173 group A HRV-positive specimens were further subjected to serotype determination by ELISA with four serotype-specific monoclonal antibodies to VP7 (ELISA-serotyping). Ninety-one specimens (81.3%) were successfully serotyped: 41 (36.6%) were serotype 1, 13 (11.6%) serotype 2, 27 (24.1%) serotype 3, and 10 (8.9%) serotype 4. The serotypes of the remaining 21 (18.8%) could not be determined. The predominant serotype of HRV that prevailed in Gifu city changed every winter: serotype 3 (63.4%) was most prevalent in the 1st winter, serotype 4 (42.9%) in the 2nd winter, and serotype 1 (64%) in the 3rd winter.
Kawamoto
H
H
Department of Virology, Gifu Prefectural Institute of Public Health.
Tanaka
H
H
Urasawa
S
S
Urasawa
T
T
Taniguchi
K
K
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces; D005759:Gastroenteritis / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D007223:Infant; D007564:Japan / Q000453:epidemiology; D011933:Reagent Kits, Diagnostic; D012401:Rotavirus / Q000145:classification* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology / Q000382:microbiology*; D012621:Seasons
D011933:Reagent Kits, Diagnostic