Structural arrangement of the decoding site of Escherichia coli ribosomes as revealed from the data on affinity labelling of ribosomes by analogs of mRNA--derivatives of oligoribonucleotides.
Using derivatives of oligoribonucleotides bearing an active group at the 5'- or 3'-end, the affinity modification of Escherichia coli ribosomes has been investigated in model complexes imitating various steps of initiation and elongation with a different extent of approximation to the real protein-synthesizing system. The protein environment of the ribosome decoding site is determined. The S3, S4, S9, L2, L7/L12 proteins belong to the 5'-region of the decoding site, and the S5, S7, S9, L1, L16 proteins to the 3'-region. In the process of translation the template moves along the external side of the 30 S subunit, from the L1 ridge to the L7/L12 stalk. The structural arrangement of the decoding site or its nearest environment depends on the functional state of ribosomes in the process of translation.
Vladimirov
S N
SN
Institute of Bioorganic Chemistry, Siberian Division, Academy of Sciences, Novosibirsk, U.S.S.R.
Babkina
G T
GT
Venijaminova
A G
AG
Gimautdinova
O I
OI
Zenkova
M A
MA
Karpova
G G
GG
D000345:Affinity Labels / Q000378:metabolism; D002250:Carbon Radioisotopes; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D007700:Kinetics; D008961:Models, Structural; D009843:Oligoribonucleotides / Q000138:chemical synthesis / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D012358:RNA, Transfer, Met; D012360:RNA, Transfer, Phe / Q000378:metabolism; D012269:Ribosomal Proteins / Q000302:isolation & purification / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism / Q000648:ultrastructure*
D000345:Affinity Labels; D002250:Carbon Radioisotopes; D009843:Oligoribonucleotides; D012333:RNA, Messenger; D012346:RNA, Transfer, Amino Acyl; D012358:RNA, Transfer, Met; D012360:RNA, Transfer, Phe; D012269:Ribosomal Proteins; C024988:tRNA, formylmethionine-