title: Bicarbonate and DIDS effects on intracellular potential difference in rabbit ciliary epithelium.
abstract: A change of [HCO3-] in the solution bathing isolated rabbit ciliary epithelium from 30 mM to 0, or vice versa, resulted in a change of intracellular electrical potential difference (PDI) of 7 mV. Intermediate [HCO3-] (5, 10 and 15 mM) solutions were also tested. No perceivable change of PDI occurred when [HCO3-] changed from 30 to 15 mM. PDI depolarized gradually when [HCO3-] decreased from 30 or 15 mM to 0 mM, but PDI hyperpolarized at a faster rate when [HCO3-] increased from 0 to 15 or 30 mM. The amount of change of PDI from 15 to 5 mM, or vice versa, and from 10 to 0 mM or vice versa, was 4 mV. The stilbene, DIDS (10(-4) M), hyperpolarized the PDI in HCO3(-)-rich solutions. The response of DIDS was reduced in HCO3(-)-free medium and inhibited in Cl(-)-free solution. The results suggest the existence of an electrogenic bicarbonate transport or Na+/HCO3- cotransport system.
authors.affiliation: Department of Ophthalmology, Medical College of Georgia, Augusta 30912-3400.
authors.forename: T C
authors.initials: TC
authors.lastname: Chu
authors.forename: K
authors.initials: K
authors.lastname: Green
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*; D000818:Animals; D001639:Bicarbonates / Q000378:metabolism / Q000494:pharmacology*; D002924:Ciliary Body / Q000187:drug effects / Q000378:metabolism*; D004594:Electrophysiology; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D008839:Microelectrodes; D010477:Perfusion; D011817:Rabbits; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D001639:Bicarbonates; D013267:Stilbenes; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid