Neuroblastoma: inverse relationship between expression of N-myc and NGF-r.
12 primary neuroblastomas (NB) of different maturation stages, 2 ganglioneuromas (GN), and 2 neuroblastoma cell lines were analysed for RNA expression of the protooncogene N-myc and the gene encoding the nerve growth factor receptor (NGF-r) by Northern-blots, RNA-dot-blots and for receptor presence by immunohistological procedures. In 4 tumors with strongly elevated RNA expression of N-myc the NGF-r RNA expression was weak or absent. In all 8 tumors with highly increased NGF-r transcription no N-myc expression was detectable. These results, indicating an inverse relationship between N-myc and NGF-r expression, could help in establishing markers for differentiation, and thus prognosis, in neuroblastoma.
Christiansen
H
H
Kinderklinik, Giessen, Federal Republic of Germany.
Christiansen
N M
NM
Wagner
F
F
Altmannsberger
M
M
Lampert
F
F
D015139:Blotting, Southern; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D005500:Follow-Up Studies; D006801:Humans; D009367:Neoplasm Staging; D009447:Neuroblastoma / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D009693:Nucleic Acid Hybridization; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes; D011956:Receptors, Cell Surface / Q000235:genetics*; D017475:Receptors, Nerve Growth Factor; D014158:Transcription, Genetic
D004273:DNA, Neoplasm; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D011956:Receptors, Cell Surface; D017475:Receptors, Nerve Growth Factor; D011505:Protein-Tyrosine Kinases