Thrombus detection using a Tc-99m labeled antifibrin monoclonal antibody (MoAb). Experiments in vitro and in animals.
This paper describes the production and characterization of an antifibrin monoclonal antibody, produced by immunizing mice with fibrinogen degradation fragment Y. The antibody (designated Y22) is directed against a conformation dependent epitope in the D-domain of fibrin Y22 was labeled with the radionuclide Tc-99m. Experiments in vitro and in rats are presented which show the potential of scintigraphic detection of thrombi with Tc-99m-Y22. Preliminary results show that immunoscintigraphy of thrombi may also have potential for the monitoring of thrombolytic therapy (eg with t-PA).
Wasser
M N
MN
Gaubius Institute TNO, Leiden, The Netherlands.
Pauwels
E K
EK
Nieuwenhuizen
W
W
D000818:Animals; D000911:Antibodies, Monoclonal; D003429:Cross Reactions / Q000276:immunology; D005337:Fibrin / Q000276:immunology*; D005342:Fibrinolysis; D006801:Humans; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D011877:Radionuclide Imaging; D051381:Rats; D011919:Rats, Inbred Strains; D013667:Technetium; D013927:Thrombosis / Q000000981:diagnostic imaging*; D014682:Vena Cava, Inferior / Q000000981:diagnostic imaging
D000911:Antibodies, Monoclonal; D013667:Technetium; D005337:Fibrin