An improved method for isolating cardiac myocytes useful for patch-clamp studies.
An improved method for isolating cardiac myocytes is described. This method consists of the initial dispersion of the myocytes with a new type of collagenase and the following treatment of the cells with a non-specific protease. This method gives more than 80% rod-shaped, viable, and quiescent cells, which are suitable for biochemical as well as electrophysiological experiments. Formation of a G omega seal and subsequent whole-cell recording is easily performed using these cells.
Yazawa
K
K
National Institute for Physiological Sciences, Okazaki, Japan.
Kaibara
M
M
Ohara
M
M
Kameyama
M
M
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D004594:Electrophysiology; D005260:Female; D006168:Guinea Pigs; D006321:Heart / Q000502:physiology; D066298:In Vitro Techniques; D003012:Microbial Collagenase; D009206:Myocardium / Q000166:cytology*; D010447:Peptide Hydrolases; D011188:Potassium / Q000378:metabolism
D010447:Peptide Hydrolases; D003012:Microbial Collagenase; D011188:Potassium