Preparative isolation of human beta 2-microglobulin from urine.
Human beta 2-microglobulin was isolated from the urine of patients with tubular proteinuria by sequential ion exchange and gel filtration chromatography on Sepharose Q fast flow and Sephacryl S-200 respectively. Large volumes of starting material could be processed and yielded a homogeneous preparation of purity 99% or greater with a recovery of about 50% of the immunochemically demonstrable beta 2-microglobulin. This method provides protein suitable for structural studies and investigation of beta 2-microglobulin amyloidogenesis.
Nelson
S R
SR
Department of Medicine, Royal Postgraduate Medical School, London, U.K.
Pepys
M B
MB
D002847:Chromatography, Agarose; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008722:Methods; D011507:Proteinuria / Q000652:urine*; D001613:beta 2-Microglobulin / Q000302:isolation & purification* / Q000652:urine
D001613:beta 2-Microglobulin