A dot-ELISA intended for the specific and simultaneous detection of antibodies directed to antigens derived from Toxoplasma gondii, rubella virus, cytomegalovirus, and type 1 and type 2 herpesviruses.
A procedure for the routine and simultaneous laboratory detection of IgG antibodies produced in humans in the course of various infectious diseases is described. The procedure, based on dot-enzyme-linked immunosorbent assay (ELISA), used single nitrocellulose strips onto which several antigens were dotted in close proximity. Optimal conditions were specified that allowed the unequivocal and simultaneous detection of IgG antibodies specifically directed against Toxoplasma gondii, rubella virus, cytomegalovirus, and herpes simplex virus type 1 and type 2 antigens. This technique has proved to be simultaneously specific, sensitive, and reliable, and it has been applied to prenatal screening of sera from pregnant women. It is suggested that this technique should also be used for the screening of large numbers of sera under field trial conditions.
Leonardi
M S
MS
Microbiology Institute, Faculties of Medicine and Surgery, Messina, Italy.
Zummo
S
S
Fattal-German
M
M
Bizzini
B
B
Mastroeni
P
P
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D003587:Cytomegalovirus / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D005260:Female; D006564:Herpesviridae / Q000276:immunology; D006801:Humans; D008403:Mass Screening; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis / Q000276:immunology / Q000517:prevention & control; D012412:Rubella virus / Q000276:immunology; D014122:Toxoplasma / Q000276:immunology
D000913:Antibodies, Protozoan; D000914:Antibodies, Viral