Dietary Ca2+ increases natriuretic and diuretic responses to volume loading in NaCl-sensitive spontaneously hypertensive rats.
The present study tests the hypothesis that dietary Ca2+ supplementation increases acute diuretic and natriuretic responses to volume loading in the NaCl-sensitive spontaneously hypertensive rat (SHR-S). Seven week old male SHR-S and normotensive Wistar-Kyoto rats (WKY) were fed one of the following diets for 2.5 weeks: basal (0.75% NaCl, 0.68% Ca2+); high NaCl (8% NaCl, 0.68% Ca2+); high Ca2+ (0.75% NaCl, 2.0% Ca2+); and high NaCl and Ca2+ (8% NaCl, 2.0% Ca2+). SHR-S on high Ca2+ and on high NaCl and Ca2+ diets for 2 weeks displayed significantly lower systolic arterial pressures (SAP) than SHR-S on basal and high NaCl diets, respectively. WKY displayed no diet-related change in SAP at any time during the study. After 2.5 weeks on the diets, pre-instrumented, conscious SHR-S and WKY received an intravenous infusion (5% body weight) of isotonic NaCl, and urine was collected through a bladder catheter for a period of 90 min. The infusion did not alter the heart rate or the mean arterial pressure in any group. WKY on the high NaCl diet excreted a significantly greater percentage of the volume and Na+ load than WKY on the basal diet. In contrast, SHR-S on the high NaCl (compared to basal) diet did not display significantly enhanced natriuresis or diuresis. SHR-S on the basal diet displayed excretion rates similar to WKY on the basal diet. Dietary Ca2+ supplementation significantly increased the natriuretic and diuretic responses to saline infusion in SHR-S on the high NaCl diet, but not in SHR-S on the basal diet or in WKY on either diet.(ABSTRACT TRUNCATED AT 250 WORDS)
Jirakulsomchok
S
S
Department of Cell Biology, University of Alabama, Birmingham 35294.
Mozaffari
M S
MS
Oparil
S
S
Wyss
J M
JM
D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D002136:Calcium, Dietary / Q000627:therapeutic use*; D004231:Diuresis / Q000502:physiology*; D006973:Hypertension / Q000178:diet therapy* / Q000503:physiopathology; D009318:Natriuresis / Q000502:physiology*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012965:Sodium Chloride; D012982:Sodium, Dietary / Q000008:administration & dosage; D014882:Water-Electrolyte Balance / Q000502:physiology
D002136:Calcium, Dietary; D012982:Sodium, Dietary; D012965:Sodium Chloride