Characterization of a Mycobacterium tuberculosis insertion sequence, IS6110, and its application in diagnosis.
An insertion sequence-like element, IS6110, was isolated from a Mycobacterium tuberculosis cosmid library as a repetitive sequence. IS6110 shows similarities with elements of the IS3 family. This insertion sequence was found to be specific to mycobacteria belonging to the M. tuberculosis complex. For detection and identification of M. tuberculosis bacilli in uncultured specimens, oligonucleotides derived from the IS6110 sequence were used as primers and probes in polymerase chain reaction studies. The results obtained were consistent with results of classical identification procedures, bacteriological data, and clinical criteria.
Thierry
D
D
Laboratoire des Sondes Froides, Centre National de la Recherche Scientifique Unité de Recherche Associée No. 1300, Institut Pasteur, Paris, France.
Brisson-Noël
A
A
Vincent-Lévy-Frébault
V
V
Nguyen
S
S
Guesdon
J L
JL
Gicquel
B
B
D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D015335:Molecular Probes; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D015335:Molecular Probes