Expression and secretion of the bovine coronavirus hemagglutinin-esterase glycoprotein by insect cells infected with recombinant baculoviruses.
A cDNA fragment representing the hemagglutinin-esterase (HE) gene of bovine coronavirus (BCV) was inserted into the genome of Autographa californica nuclear polyhedrosis virus. Infection of insect cells with the recombinant virus resulted in the production of a 120-kilodalton disulfide-linked dimeric form of the BCV HE polypeptide. Deletion of the carboxy-terminal hydrophobic domain from the HE polypeptide resulted in secretion of a dimeric form of the truncated HE polypeptide. The acetylesterase activity of the BCV HE was detectable in insect cells expressing the BCV hemagglutinin and was inhibited by two monoclonal antibodies which also inhibit hemagglutination.
Parker
M D
MD
Veterinary Infectious Disease Organization, Saskatoon, Saskatchewan, Canada.
Yoo
D
D
Babiuk
L A
LA
D000115:Acetylesterase / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003332:Coronaviridae / Q000235:genetics*; D005822:Genetic Vectors; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000378:metabolism*; D007304:Insect Viruses / Q000235:genetics*; D007313:Insecta / Q000235:genetics*; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D014760:Viral Fusion Proteins; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D014760:Viral Fusion Proteins; D014764:Viral Proteins; C060814:hemagglutinin esterase; D000115:Acetylesterase