Homology between feline herpesvirus-1 and canine herpesvirus.
The purpose of this study was to determine the extent of the relatedness between feline herpesvirus-1 (FHV-1) and canine herpesvirus (CHV). Immunoprecipitation studies using antisera to FHV-1 and CHV revealed that both share virion glycoprotein antigens with apparent molecular weights of approximately 60 and 68 kDa. Two non-glycosylated, virion-associated antigens of each virus also displayed weaker cross reactivity. Southern blot hybridization experiments indicated that restriction fragments which represented approximately 51% of the FHV-1 genome hybridized to CHV DNA under conditions which allowed less than 7% base pair mismatch. This is the first molecular characterization of cross-reactivity between FHV-1 and another herpesvirus.
Rota
P A
PA
Department of Microbiology and Public Health, Michigan State University, East Lansing.
Maes
R K
RK
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology; D015139:Blotting, Southern; D002371:Cat Diseases / Q000382:microbiology*; D002415:Cats; D003429:Cross Reactions; D004279:DNA, Viral / Q000032:analysis; D004283:Dog Diseases / Q000382:microbiology*; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D006564:Herpesviridae / Q000235:genetics* / Q000276:immunology; D006566:Herpesviridae Infections / Q000382:microbiology / Q000662:veterinary*; D002184:Herpesvirus 1, Canid / Q000235:genetics* / Q000276:immunology; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D000956:Antigens, Viral; D004279:DNA, Viral