Localization of the receptor gene for type D simian retroviruses on human chromosome 19.
Simian retrovirus (SRV) serotypes 1 to 5 are exogenous type D viruses causing immune suppression in macaque monkeys. These viruses exhibit receptor interference with each other, with two endogenous type D viruses of the langur (PO-1-Lu) and squirrel monkey, and with two type C retroviruses, feline endogenous virus (RD114/CCC) and baboon endogenous virus (BaEV), indicating that each utilizes the same cell surface receptor (M. A. Sommerfelt and R. A. Weiss, Virology 176:58-69, 1990). Vesicular stomatitis virus pseudotype particles bearing envelope glycoproteins of RD114, BaEV, and the seven SRV strains were employed to detect receptors expressed in human-rodent somatic cell hybrids segregating human chromosomes. The only human chromosome common to all the susceptible hybrids was chromosome 19. By using hybrids retaining different fragments of chromosome 19, a provisional subchromosomal localization of the receptor gene was made to 19q13.1-13.2. Antibodies previously reported to be specific to a BaEV receptor (L. Thiry, J. Cogniaux-Leclerc, R. Olislager, S. Sprecher-Goldberger, and P. Burkens, J. Virol. 48:697-708, 1983) did not block BaEV, RD114, or SRV pseudotypes or syncytia. Antibodies to known surface markers determined by genes mapped to chromosome 19 did not block virus-receptor interaction. The identity of the receptor remains to be determined.
Sommerfelt
M A
MA
Chester Beatty Laboratories, Institute of Cancer Research, London, United Kingdom.
Williams
B P
BP
McKnight
A
A
Goodfellow
P N
PN
Weiss
R A
RA
D000818:Animals; D002460:Cell Line; D002520:Cercopithecidae; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D006224:Cricetinae; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology; D008251:Macaca; D051379:Mice; D011991:Receptors, Virus / Q000235:genetics* / Q000502:physiology; D012190:Retroviridae / Q000502:physiology*; D012453:Saimiri; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics
D011991:Receptors, Virus