Identification of the major immunogenic structural proteins of human foamy virus.
We have identified the major immunogenic structural proteins of the human foamy virus (HFV), a distinct member of the foamy virus subfamily of Retroviridae. Radiolabelled viral proteins were immunoprecipitated from HFV-infected cells by foamy virus antisera of human and non-human primate origin. Precipitated viral proteins were in the range of 31K to 170K. Labelling of proteins with [14C]glucosamine or with [35S]methionine in the presence of tunicamycin, as well as endo-beta-N-acetylglycosaminidase H and F treatment of [35S]methionine-labelled proteins, revealed three viral glycoproteins of approximately 170K, 130K and 47K, most likely representing the env gene-encoded precursor, the surface glycoprotein and the transmembrane protein of HFV, respectively.
Netzer
K O
KO
Institut für Virologie und Immunbiologie, Universität Würzburg, F.R.G.
Rethwilm
A
A
Maurer
B
B
ter Meulen
V
V
D000956:Antigens, Viral / Q000032:analysis; D015686:Gene Products, env / Q000032:analysis; D006801:Humans; D007106:Immune Sera; D011233:Precipitin Tests; D011498:Protein Precursors / Q000032:analysis; D012190:Retroviridae / Q000032:analysis*; D016092:Spumavirus / Q000032:analysis* / Q000276:immunology; D014407:Tumor Cells, Cultured; D015678:Viral Structural Proteins / Q000032:analysis* / Q000276:immunology
D000956:Antigens, Viral; D015686:Gene Products, env; D007106:Immune Sera; D011498:Protein Precursors; D015678:Viral Structural Proteins