Protein transfer into the recipient cell during bacterial conjugation: studies with F and RP4.
Transfer of donor cell proteins to the recipient bacterium was examined in F- and RP4-mediated conjugation. Transfer of a 120 kD polypeptide, identified as the larger product of the plasmid DNA primase gene, was readily detected during RP4-promoted conjugation. The protein was transmitted to the cytoplasm of the recipient, presumably complexed to the transferred ssDNA. F DNA was transferred without detectable association with any cytoplasmic tra protein or with the ssDNA-binding protein encoded by the plasmid. However, a 92 kD protein, possibly F TraD product, was transmitted to the membrane fraction of the recipient cell.
Rees
C E
CE
Department of Genetics, University of Leicester, UK.
Wilkins
B M
BM
D001426:Bacterial Proteins / Q000378:metabolism*; D003227:Conjugation, Genetic; D004265:DNA Helicases / Q000378:metabolism; D019915:DNA Primase; D004277:DNA, Single-Stranded / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D029968:Escherichia coli Proteins; D005144:F Factor; D005798:Genes, Bacterial; D015151:Immunoblotting; D011815:R Factors; D012316:RNA Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D004277:DNA, Single-Stranded; D029968:Escherichia coli Proteins; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases; C027093:TraI protein, E coli; D004265:DNA Helicases