Molecular analysis of the mitochondrial transcription factor mtf2 of Saccharomyces cerevisiae.
The nuclear gene for a new mitochondrial transcription factor (mtf2) was isolated by transformation of mutant pet-ts3504. It was localized on a 6.4 kb fragment of yeast genomic DNA by subcloning and complementation tests. Sequencing of a 1.7 kb DNA fragment revealed an open reading frame of 1320 bp. A transcript of 1400 nucleotides can be assigned to this region. Gene disruption of this reading frame in a wild-type yeast strain created a stable pet phenotype. Further analysis of this insertion mutation showed that it is allelic to the mutated gene of pet-ts3504. Comparison of the 5' upstream regions of MTF2 and a previously characterized mitochondrial transcription factor (MTF1) revealed common sequence motifs which may be important for coordinated regulation of gene expression.
Lisowsky
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Botanisches Institut, Universität Düsseldorf, Federal Republic of Germany.
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D003433:Crosses, Genetic; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D010641:Phenotype; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics*; D014170:Transformation, Genetic
C063626:MTF2 protein, S cerevisiae; D024101:Mitochondrial Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors