Dye-stained gelatin microcarriers as insoluble chromolytic substrates for the determination of proteolytic activity.
Dye-stained gelatin microcarriers were used as insoluble chromolytic substrates for the determination of proteolytic activity. Trypsin at concentration 100 ng/ml could be easily detected. The spontaneous leakage of dyed fragments into water solutions was negligible.
Safarík
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Laboratory of Analytical Chemistry, South Bohemian Biological Centre, Ceské Budĕjovice, Czechoslovakia.
D004396:Coloring Agents; D005780:Gelatin; D008823:Micelles; D010447:Peptide Hydrolases / Q000032:analysis*
D004396:Coloring Agents; D008823:Micelles; D005780:Gelatin; D010447:Peptide Hydrolases