Increase in lactate dehydrogenase isoenzyme-1 in plasma in pediatric malignancy.
We investigated 28 cases of pediatric malignancy in which total lactate dehydrogenase (LD, EC 1.1.1.27) activities were increased and isoenzyme LD-1 exceeded LD-2 (flipped pattern). Of these, 11 had a flipped pattern at presentation and 17 showed a flipped pattern during chemotherapy. Those with flipped patterns at presentation were four with germ-cell tumors, one with acute lymphocytic leukemia, and six with nephroblastomas (Wilms tumor). Four of five nephroblastoma homogenates contained predominantly LD-1; one revealed a structurally normal LD-1 with normal kinetics. We conclude that an increase in LD with a flipped pattern is common in nephroblastoma and, in addition, may develop in cancer patients treated with chemotherapy.
Sacks
S S
SS
Department of Chemical Pathology, Groote Schuur Hospital, Cape Town, South Africa.
Mulligan
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G
D014408:Biomarkers, Tumor / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007527:Isoenzymes; D007680:Kidney Neoplasms / Q000097:blood / Q000201:enzymology*; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D009396:Wilms Tumor / Q000097:blood / Q000201:enzymology*
D014408:Biomarkers, Tumor; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase