Neuropeptide Y inhibition of vasoactive intestinal polypeptide-stimulated ion transport in the rabbit distal colon.
We have studied the effect of neuropeptide Y on basal and vasoactive intestinal polypeptide-stimulated changes in the short-circuit current of strips of colonic mucosa from New Zealand white rabbits mounted in Ussing chambers. When administered to the basolateral surface, neuropeptide Y is found to decrease basal short-circuit current. Neuropeptide Y inhibits vasoactive intestinal peptide-stimulated increases in short-circuit current in a concentration-dependent fashion by a tetrodotoxin-insensitive mechanism. Also, neuropeptide Y inhibited increases in short-circuit current produced by direct stimulation of adenylate cyclase with forskolin. Furthermore, neuropeptide Y prevents vasoactive intestinal peptide-stimulated increases in tissue cyclic adenosine monophosphate levels. These results indicate that neuropeptide Y administered to the basolateral membrane inhibits vasoactive intestinal peptide-stimulated short-circuit current changes by a tetrodotoxin-insensitive mechanism that decreases tissue levels of cyclic adenosine 3',5'-monophosphate.
Flint
J S
JS
St Mary's Hospital, Waterbury, Conn.
Ballantyne
G H
GH
Goldenring
J R
JR
Fielding
L P
LP
Modlin
I M
IM
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D005576:Colforsin / Q000494:pharmacology; D003106:Colon / Q000187:drug effects*; D000242:Cyclic AMP / Q000378:metabolism; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000187:drug effects*; D009478:Neuropeptide Y / Q000494:pharmacology*; D011817:Rabbits; D013779:Tetrodotoxin / Q000494:pharmacology; D014660:Vasoactive Intestinal Peptide / Q000037:antagonists & inhibitors*
D009478:Neuropeptide Y; D005576:Colforsin; D014660:Vasoactive Intestinal Peptide; D013779:Tetrodotoxin; D000242:Cyclic AMP