Isolation and purification of a non-A, non-B hepatitis-associated microtubular aggregates protein.
Blood-borne type non-A, non-B (NANB) hepatitis-associated microtubular aggregates protein was isolated and partially sequenced. The microtubular aggregates were isolated from the hepatocytes of NANB-infected chimpanzees and were found to have a buoyant density in sucrose solution of 1.21 to 1.23 g/ml. A single protein, recognized by our anti-microtubular aggregates monoclonal antibodies, was found to have an Mr of 44,000 (p44). This p44 protein was not found in uninfected chimpanzees. We determined a partial amino acid sequence for p44, and showed that it has no homology to any known proteins.
Honda
Y
Y
Department of Microbiology, Nihon University School of Medicine, Tokyo, Japan.
Kondo
J
J
Maeda
T
T
Yoshiyama
Y
Y
Yamada
E
E
Shimizu
Y K
YK
Shikata
T
T
Ono
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000737:chemistry / Q000302:isolation & purification*; D002499:Centrifugation, Density Gradient; D002851:Chromatography, High Pressure Liquid; D003598:Cytoskeletal Proteins; D016174:Hepacivirus / Q000302:isolation & purification*; D006526:Hepatitis C / Q000473:pathology; D008099:Liver / Q000382:microbiology / Q000648:ultrastructure; D008870:Microtubules / Q000648:ultrastructure; D008969:Molecular Sequence Data; D008970:Molecular Weight; D002679:Pan troglodytes; D010446:Peptide Fragments / Q000302:isolation & purification
D000941:Antigens; D003598:Cytoskeletal Proteins; C065888:IFI44 protein, human; D010446:Peptide Fragments