Poliovirus mutants resistant to neutralization with soluble cell receptors.
Poliovirus mutants resistant to neutralization with soluble cellular receptor were isolated. Replication of soluble receptor-resistant (srr) mutants was blocked by a monoclonal antibody directed against the HeLa cell receptor for poliovirus, indicating that the mutants use this receptor to enter cells. The srr mutants showed reduced binding to HeLa cells and cell membranes. However, the reduced binding phenotype did not have a major impact on viral replication, as judged by plaque size and one-step growth curves. These results suggest that the use of soluble receptors as antiviral agents could lead to the selection of neutralization-resistant mutants that are able to bind cell surface receptors, replicate, and cause disease.
Kaplan
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Department of Microbiology, College of Physicians and Surgeons of Columbia University, New York, NY 10032.
Peters
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D
Racaniello
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VR
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000998:Antiviral Agents; D016367:Baculoviridae / Q000235:genetics; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000235:genetics; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D007313:Insecta; D009154:Mutation; D009500:Neutralization Tests; D017955:Poliovirus / Q000235:genetics / Q000502:physiology*; D011991:Receptors, Virus / Q000235:genetics / Q000502:physiology*; D011994:Recombinant Proteins / Q000502:physiology; D014162:Transfection; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D000998:Antiviral Agents; D011991:Receptors, Virus; D011994:Recombinant Proteins; D004247:DNA