Monoclonal antibodies against Epstein-Barr virus transformed B lymphocytes from a CLL patient.
Immunization of BALB/c mice with EBV-CLL-1 cells, derived from Epstein-Barr virus transformed B lymphocytes from a chronic lymphocytic leukemia (CLL) patient, yielded 2 monoclonal antibodies (IgG1 Kappa and IgG2a Kappa) against a membrane antigen on a subset of normal B lymphocytes and non-Hodgkin's lymphomas. Immunofluorescence revealed strong reactivity of the antibodies with EBV-CLL-1 cells and with most lymphocytes in tonsil follicles, in the intestinal wall, around splenic arterioles and near Hassall's corpuscles in the neonatal thymus as well as with a small proportion of lymphocytes in some large reactive lymph node follicles, weak reactivity with 1/5 of peripheral blood B lymphocytes (PBL), and no reactivity with platelets, granulocytes and non-lymphoid tissues. PBL from 3 CLL patients showed weak staining of only larger cells. Intense fluorescence was observed in several non-Hodgkin's lymphomas of various histological types and in Burkitt's lymphoma lines but not in the 3 T lymphoblastoid and 12 nonlymphoid tumor lines examined. The antibodies precipitated Mr 22,000 and 33,000 bands from surface labeled RAJI or EBV-CLL-1 cells and cross-competed in a binding inhibition assay. The antibodies had approximately 6 million binding sites per EBV-CLL-1 or RAJI cell but were not cytotoxic. This high antigen-density and limited expression in normal cells may permit their use for immunocytological diagnosis and targeting cytotoxic agents and radionuclides against appropriate lymphoma cells.
Guha
A K
AK
Department of Pathology, Dalhousie University, Halifax, Nova Scotia, Canada.
Ghose
T
T
Luner
S J
SJ
Nolido-Cruz
H
H
Uniyal
S
S
Rajaraman
R
R
Fernandez
L A
LA
Lee
S S
SS
Lee
C L
CL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis* / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000276:immunology*; D001667:Binding, Competitive; D002461:Cell Line, Transformed; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009928:Organ Specificity; D011233:Precipitin Tests
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface