title: Persistent B19 parvovirus infection in patients infected with human immunodeficiency virus type 1 (HIV-1): a treatable cause of anemia in AIDS.
abstract: OBJECTIVE
  To determine the role of B19 parvovirus in red cell aplasia of patients infected with human immunodeficiency virus type 1 (HIV-1).
  
  
  METHODS
  Uncontrolled clinical trial, with assay of serum, peripheral blood cells, and bone marrow for virus using DNA hybridization and immunocytochemistry techniques; these assays were then correlated with clinical findings, results of immunoassays for antivirus antibodies, and with immunoglobulin (Ig) therapy.
  
  
  METHODS
  Government medical referral center, and university and private hospitals.
  
  
  METHODS
  Seven patients with pure red cell aplasia and serologic evidence of infection with HIV-1.
  
  
  RESULTS
  All patients had giant pronormoblasts in the bone marrow (present in transient aplastic crisis caused by acute B19 parvovirus infection). High concentrations of B19 parvovirus were demonstrated in sera, in several cases in samples separated by weeks or months. Viral DNA and capsid protein were present in the bone marrow of three patients studied, and active viral replication was detected by southern analysis. There was no antivirus IgG in capture immunoassay and no or very low levels of antivirus IgM. The patients did not have symptoms of fifth disease, the illness caused by this virus in immunologically normal persons. Six patients were treated with a regimen of intravenous commercial immunoglobulin. In all cases, this therapy resulted in rapid reduction in serum virus concentrations and full recovery of erythropoiesis. Relapses in two cases were predicted by DNA hybridization studies, and these cases were successfully retreated.
  
  
  CONCLUSIONS
  The B19 parvovirus is a remediable cause of severe chronic anemia in HIV-infected patients. Recognition of and therapy for parvovirus in this population will avoid erythrocyte transfusion and should prevent transmission of the virus to other persons, including immunosuppressed persons and women of child-bearing age.
authors.affiliation: National Heart, Lung, and Blood Institute, Bethesda, Maryland.
authors.forename: N
authors.initials: N
authors.lastname: Frickhofen
authors.forename: J L
authors.initials: JL
authors.lastname: Abkowitz
authors.forename: M
authors.initials: M
authors.lastname: Safford
authors.forename: J M
authors.initials: JM
authors.lastname: Berry
authors.forename: J
authors.initials: J
authors.lastname: Antunez-de-Mayolo
authors.forename: A
authors.initials: A
authors.lastname: Astrow
authors.forename: R
authors.initials: R
authors.lastname: Cohen
authors.forename: I
authors.initials: I
authors.lastname: Halperin
authors.forename: L
authors.initials: L
authors.lastname: King
authors.forename: D
authors.initials: D
authors.lastname: Mintzer
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000150:complications*; D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis; D001769:Blood / Q000382:microbiology; D001853:Bone Marrow / Q000382:microbiology; D004279:DNA, Viral / Q000032:analysis; D015497:HIV-1; D006801:Humans; D007074:Immunoglobulin G / Q000627:therapeutic use; D008297:Male; D010321:Parvoviridae / Q000302:isolation & purification; D010322:Parvoviridae Infections / Q000150:complications* / Q000175:diagnosis / Q000628:therapy; D012367:RNA, Viral / Q000032:analysis; D012010:Red-Cell Aplasia, Pure / Q000209:etiology*
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D007074:Immunoglobulin G; D012367:RNA, Viral