Expression of aldolase C isozyme in renal cell carcinoma.
The authors localized aldolase C in renal tubules and renal cell carcinoma by immunohistochemical study and quantitative analysis by an enzyme immunoassay. Aldolase C was localized in epithelial cells of loops of Henle and collecting ducts and in those of Bowman's capsules. In renal cell carcinoma, aldolase C was immunohistochemically demonstrated in 95% (41 of 43) of cases, including one sarcomatoid variant. The tissue concentrations of aldolase C in the renal cortex (n = 8) were 12.7 +/- 6.2 micrograms/g protein (mean +/- standard deviation), and those of the medulla (n = 8) were 20.3 +/- 6.9 micrograms/g protein. On the other hand, the concentrations in renal cell carcinoma (n = 26) were 93.5 +/- 95.9 micrograms/g protein: about seven times higher than that in renal cortex (P less than 0.001). These findings indicate that aldolase C was first expressed in renal cell carcinoma, which is derived from proximal renal tubules, because proximal renal tubules had aldolase B but not aldolase C.
Takashi
M
M
Department of Urology, Nagoya University School of Medicine, Japan.
Haimoto
H
H
Koshikawa
T
T
Kato
K
K
D002292:Carcinoma, Renal Cell / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D005634:Fructose-Bisphosphate Aldolase / Q000032:analysis* / Q000097:blood; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007527:Isoenzymes / Q000032:analysis* / Q000097:blood; D007668:Kidney / Q000201:enzymology; D007680:Kidney Neoplasms / Q000201:enzymology*; D007684:Kidney Tubules / Q000201:enzymology*; D012016:Reference Values
D007527:Isoenzymes; D005634:Fructose-Bisphosphate Aldolase