title: Heavy chain binding protein recognizes incompletely disulfide-bonded forms of vesicular stomatitis virus G protein.
abstract: To investigate the function of heavy chain binding protein (BiP, GRP 78) in the endoplasmic reticulum, we have characterized its interaction with a model plasma membrane glycoprotein, the G protein of vesicular stomatitis virus. We used a panel of well characterized mutant G proteins and immunoprecipitation with anti-BiP antibodies to determine if BiP interacted with newly synthesized G protein and/or mutant G proteins retained in the endoplasmic reticulum. We made three major observations: 1) BiP bound transiently to folding intermediates of wild-type G protein which were incompletely disulfide-bonded; 2) BiP did not bind stably to all mutant G proteins which remain in the endoplasmic reticulum; and 3) BiP bound stably only to mutant G proteins which do not form correct intrachain disulfide bonds.
authors.affiliation: Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510.
authors.forename: C E
authors.initials: CE
authors.lastname: Machamer
authors.forename: R W
authors.initials: RW
authors.lastname: Doms
authors.forename: D G
authors.initials: DG
authors.lastname: Bole
authors.forename: A
authors.initials: A
authors.lastname: Helenius
authors.forename: J K
authors.initials: JK
authors.lastname: Rose
mesh_terms: D000818:Animals; D002460:Cell Line; D002872:Chromosome Deletion; D004220:Disulfides / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006031:Glycosylation; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; D009154:Mutation; D011485:Protein Binding; D014162:Transfection; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D004220:Disulfides; C028391:G protein, vesicular stomatitis virus; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins