title: Dissemination of amikacin resistance gene aphA6 in Acinetobacter spp.
abstract: The distribution of the aphA6 gene, encoding a 3'-aminoglycoside phosphotransferase type VI, was studied by dot blot hybridization with 115 amikacin-resistant Acinetobacter strains from various geographical areas. Nucleotide sequences related to aphA6 were found in 109 strains belonging to seven species. As inferred from results of Southern hybridization, dissemination of amikacin resistance in Acinetobacter spp. is due to a gene rather than a strain or plasmid epidemic.
authors.affiliation: Unité des Agents Antibactériens, Institut Pasteur, Paris, France.
authors.forename: T
authors.initials: T
authors.lastname: Lambert
authors.forename: G
authors.initials: G
authors.lastname: Gerbaud
authors.forename: P
authors.initials: P
authors.lastname: Bouvet
authors.forename: J F
authors.initials: JF
authors.lastname: Vieu
authors.forename: P
authors.initials: P
authors.lastname: Courvalin
mesh_terms: D000150:Acinetobacter / Q000187:drug effects / Q000235:genetics*; D000583:Amikacin / Q000494:pharmacology*; D015139:Blotting, Southern; D003227:Conjugation, Genetic / Q000502:physiology; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial / Q000502:physiology*; D005819:Genetic Markers; D019868:Kanamycin Kinase; D008826:Microbial Sensitivity Tests; D009693:Nucleic Acid Hybridization; D010770:Phosphotransferases / Q000235:genetics; D014170:Transformation, Genetic / Q000502:physiology
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D005819:Genetic Markers; D000583:Amikacin; D010770:Phosphotransferases; D019868:Kanamycin Kinase