Rapid titration of retroviral vectors encoding intracellular antigens by flow cytometry.
Flow cytometry was used to detect cells infected with retroviral vectors encoding both simian virus 40 large T antigen and G418 resistance after indirect immunofluorescence staining using a T-antigen-specific monoclonal antibody and a fluorescein-conjugated secondary antibody. Titers of viral stocks determined by flow cytometry were equivalent to those determined by quantitation of G418-resistant colonies.
Sladek
T L
TL
Department of Genetics, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.
Jacobberger
J W
JW
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000911:Antibodies, Monoclonal; D000952:Antigens, Polyomavirus Transforming / Q000032:analysis / Q000235:genetics*; D002453:Cell Cycle / Q000187:drug effects; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004351:Drug Resistance / Q000235:genetics*; D005434:Flow Cytometry / Q000379:methods; D005455:Fluorescent Antibody Technique; D005822:Genetic Vectors; D005839:Gentamicins / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D012190:Retroviridae / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics* / Q000276:immunology
D000900:Anti-Bacterial Agents; D000911:Antibodies, Monoclonal; D000952:Antigens, Polyomavirus Transforming; D005839:Gentamicins; C010680:antibiotic G 418