Subsets of pituitary intermediate lobe cells bind CRH and secrete ACTH/CLIP in a reverse hemolytic plaque assay.
Dissociated neurointermediate lobe cells (75%) bound [biotinyl-Ser1]corticotropin releasing hormone (bio-CRH) and secreted adrenocorticotropin [ACTH(25-39)] in a reverse hemolytic plaque assay (RHPA). The bio-CRH was rapidly internalized (less than 1 min) in vesicles and scattered vacuoles. Plaque percentages increased from 26 +/- 4% to 53 +/- 8% after CRH stimulation. Areas increased from 1688 +/- 791 microns 2 to 3773 +/- 788 microns 2. After AVP treatment for 1 hr, plaque percentages and areas were augmented further. The heterogeneity in secretion may indicate the presence of quiescent IL cells or cells secreting an opiocortin peptide not detected in the plaque assay.
Childs
G V
GV
Department of Anatomy and Neurosciences, University of Texas Medical Branch, Galveston 77551.
D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D000818:Animals; D001710:Biotin / Q000378:metabolism; D053497:Corticotropin-Like Intermediate Lobe Peptide; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism*; D006462:Hemolytic Plaque Technique; D008297:Male; D010446:Peptide Fragments / Q000378:metabolism*; D010902:Pituitary Gland / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains
D010446:Peptide Fragments; D053497:Corticotropin-Like Intermediate Lobe Peptide; D001710:Biotin; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone