An electron paramagnetic resonance probe to detect local Z-DNA conformations.
We present spectroscopic evidence for an electron paramagnetic resonance (EPR) probe to detect local Z-DNA conformations in synthetic DNA. A spin labeled deoxycytidine-5'-triphosphate (pppDCAT) was co-polymerized with Micrococcus luteus DNA polymerase to yield the spin active alternating co-polymer (dG-dC,DCAT)n. The EPR spectrum of (dG-dC,DCAT)n in the Z-DNA conformation indicates a decrease in the local base dynamics by about a factor of two as compared to that computed for B-DNA. A control experiment conducted with (dA-dT, DUAT)n under similar salt conditions rules out the possibility of observing salt induced artifacts.
Strobel
O K
OK
Department of Chemistry, University of Cincinnati, Ohio 45221.
Keyes
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Bobst
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D001483:Base Sequence; D003196:Computer Graphics; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D008837:Micrococcus / Q000201:enzymology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011089:Polydeoxyribonucleotides / Q000138:chemical synthesis; D013113:Spin Labels
D011089:Polydeoxyribonucleotides; D013113:Spin Labels; D004247:DNA; D004259:DNA-Directed DNA Polymerase