Differentiation of human monocytes into accessory cells at serum-free conditions.
Human peripheral blood monocytes (HPBM) cultured in the absence of serum were found to convert into a state of high accessory function which was expressed by their ability to support lymphocyte proliferation. After a prolonged culture in serum-free media, the monocyte-derived cells were highly viable, increased in size, and had developed veils and dendritiform elongations. Paralleling the increase in accessory function, we found a decrease in the expression of markers typical of monocytes and macrophages (Mø), approaching the phenotype of lymphoid dendritic cells. We here define conditions for reproducibly generating these monocyte-derived accessory cells (m-AC) in various serum-free media, thus offering a novel differentiation model at highly defined culture conditions.
Najar
H M
HM
Department of Immunology, University of Göttingen, Federal Republic of Germany.
Bru-Capdeville
A C
AC
Gieseler
R K
RK
Peters
J H
JH
D000938:Antigen-Presenting Cells / Q000166:cytology* / Q000187:drug effects; D000954:Antigens, Surface / Q000032:analysis; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured / Q000166:cytology* / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology; D003713:Dendritic Cells / Q000166:cytology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007213:Indomethacin / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008264:Macrophages / Q000502:physiology; D008934:Mitogens / Q000494:pharmacology; D009000:Monocytes / Q000166:cytology* / Q000187:drug effects; D010504:Periodic Acid / Q000494:pharmacology; D010949:Plasma / Q000502:physiology
D000954:Antigens, Surface; D003470:Culture Media; D008934:Mitogens; D010504:Periodic Acid; C009288:metaperiodate; D007213:Indomethacin