Toluene diisocyanate-induced conformational changes of serum albumin: a study on repeated inhalations in guinea-pigs.
High responder lines of Hartley guinea-pigs were sensitized by repeated inhalations of toluene diisocyanate (TDI). After 3 weeks, we demonstrated a degree of TDI substitution of the serum-albumin-enriched fraction (AEF) and we ascertained the sensitization of the most exposed animals using PCA methodology. Fourier transform infrared spectroscopy (FT-IR), used to investigate conformational changes in AEF, highlighted the structural modifications of the native protein conformation. Such crucial changes may support, at least in part, the relationship between TDI exposure and triggering of hypersensitivity reactions.
Kochman
S
S
Laboratoire de Médecine Expérimentale, Faculté de Médecine, Centre Hospitalier Universitaire, Reims, France.
Lefebvre
S
S
Bernard
J
J
Maujean
A
A
Cazabat
A
A
Lavaud
F
F
Manfait
M
M
D000280:Administration, Inhalation; D000818:Animals; D000917:Antibody Formation; D003485:Cyanates / Q000633:toxicity*; D003877:Dermatitis, Contact / Q000209:etiology; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D008297:Male; D011487:Protein Conformation; D012709:Serum Albumin / Q000032:analysis* / Q000276:immunology; D013055:Spectrophotometry, Infrared; D014051:Toluene 2,4-Diisocyanate / Q000008:administration & dosage / Q000633:toxicity*
D003485:Cyanates; D012709:Serum Albumin; D014051:Toluene 2,4-Diisocyanate