Antibodies against rabbit omega-hydroxylases of prostaglandins and fatty acids cross-react with leukotriene B4 omega-hydroxylase in human neutrophils (cytochrome P-450LTB omega).
Leukotriene B4 (LTB4) omega-hydroxylase activity in human neutrophil microsomes was significantly inhibited by antisera against three rabbit omega-hydroxylase P-450s, lung prostaglandin omega-hydroxylase (P-450p-2), small intestine prostaglandin A omega-hydroxylase (P-450ia), and kidney fatty acid omega-hydroxylase (P-450kd). In contrast, the activity is not affected by antibodies raised against the phenobarbital-inducible forms of P-450s from both rabbits and rats. These findings suggest that the LTB4 omega-hydroxylase (P-450LTB omega) is structurally related to a group of rabbit omega-hydroxylase P-450s. The antiserum raised against P-450p-2 also inhibited the NADPH-dependent oxidation of 20-hydroxy LTB4 to 20-oxo LTB4 and 20-carboxy LTB4 by the microsomes, supporting that P-450LTB omega is able to catalyze the subsequent oxidation of 20-hydroxy LTB4 as well as the omega-hydroxylation of LTB4.
Sumimoto
H
H
Department of Biochemistry, Kyushu University School of Medicine, Japan.
Kikuta
Y
Y
Kusunose
E
E
Kusunose
M
M
Minakami
S
S
D000818:Animals; D000906:Antibodies / Q000276:immunology; D003429:Cross Reactions; D042926:Cytochrome P-450 CYP4A; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000276:immunology* / Q000378:metabolism; D000072470:Cytochrome P450 Family 4; D005227:Fatty Acids / Q000378:metabolism; D006801:Humans; D007975:Leukotriene B4 / Q000378:metabolism; D008861:Microsomes / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D009504:Neutrophils / Q000201:enzymology*; D010084:Oxidation-Reduction; D011453:Prostaglandins / Q000378:metabolism; D011817:Rabbits
D000906:Antibodies; D065607:Cytochrome P-450 Enzyme Inhibitors; D005227:Fatty Acids; D011453:Prostaglandins; D007975:Leukotriene B4; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D000072470:Cytochrome P450 Family 4; C026961:prostaglandin omega hydroxylases; C040780:leukotriene B4 20-hydroxylase; D042926:Cytochrome P-450 CYP4A