Murine interleukin 2 receptor beta chain: dysregulated gene expression in lymphoma line EL-4 caused by a promoter insertion.
The functional, high-affinity interleukin 2 receptor (IL-2R) consists of at least two receptor components, IL-2R alpha (p55) and IL-2R beta (p70-75). The cDNA encoding the murine IL-2R beta has been isolated by using the previously cloned cDNA for human IL-2R beta as a probe. Analysis of the cDNA revealed that the murine IL-2R beta shows a marked homology with the human IL-2R beta and that it is also structurally related to other cytokine receptors such as erythropoietin receptor. The cDNA-directed murine IL-2R beta formed high-affinity IL-2R in conjunction with the endogenous IL-2R alpha in a murine pro-B-cell line and could transduce IL-2-induced growth signal. In mouse lymphoma line EL-4, the IL-2R beta gene was found to be rearranged by the insertion of the long terminal repeat sequence of an intracisternal A particle, giving rise to constitutive expression of the IL-2R beta mRNA.
Kono
T
T
Institute for Molecular and Cellular Biology, Osaka University, Japan.
Doi
T
T
Yamada
G
G
Hatakeyama
M
M
Minamoto
S
S
Tsudo
M
M
Miyasaka
M
M
Miyata
T
T
Taniguchi
T
T
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004273:DNA, Neoplasm / Q000235:genetics; D005786:Gene Expression Regulation; D015723:Gene Library; D008223:Lymphoma; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D015375:Receptors, Interleukin-2 / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D004251:DNA Transposable Elements; D004273:DNA, Neoplasm; D046911:Macromolecular Substances; D015375:Receptors, Interleukin-2