Expression of biologically active human platelet-derived growth factor (PDGF) B-receptor in Xenopus laevis oocytes.
Platelet-derived growth factor (PDGF) consists of three different isoforms, PDGF-AA, PDGF-AB and PDGF-BB, which bind to at least two types of receptors: the B-receptor, to which only PDGF-BB binds, and the A/B receptor, to which all three isoforms bind. Microinjection of synthetic mRNA in Xenopus laevis oocytes was used to obtain cell-surface expression of the human PDGF B-receptor. The production of receptor molecules of correct size (190 kd) was demonstrated by specific immunoprecipitation; the binding properties of the membrane- associated PDGF B-receptor were investigated with highly purified recombinant [125I] labeled human PDGF-BB and -AA. Unlike Swiss mouse 3T3 cells, which possess both B- and A/B-receptors and, therefore, bind both isoforms with high affinity, the mRNA-injected oocytes bound only the BB isoform. Mock-injected oocytes showed no specific binding.
Gassmann
M
M
Central Research Units, F. Hoffmann-La Roche & Co. Ltd. Basel, Switzerland.
Hosang
M
M
Mous
J
J
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D015870:Gene Expression; D005796:Genes; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D008845:Microinjections; D009865:Oocytes / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D017479:Receptors, Platelet-Derived Growth Factor; D014158:Transcription, Genetic; D014982:Xenopus laevis
D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017479:Receptors, Platelet-Derived Growth Factor