title: Expression of the Cellulomonas fimi cellulase genes cex and cenA from the divergent tet promoters of transposon Tn10.
abstract: A cartridge was constructed which contained the divergent tet promoters of transposon Tn10 between an exoglucanase gene (cex) and an endoglucanase gene (cenA) of Cellulomonas fimi. When carried in a broad-host-range vector, the cartridge gave expression of cex and cenA in Escherichia coli, Rhodobacter capsulatus and Klebsiella pneumoniae.
authors.affiliation: Department of Microbiology, University of British Columbia, Vancouver, Canada.
authors.forename: N
authors.initials: N
authors.lastname: Din
authors.forename: C F
authors.initials: CF
authors.lastname: Beck
authors.forename: R C
authors.initials: RC
authors.lastname: Miller
authors.forename: D G
authors.initials: DG
authors.lastname: Kilburn
authors.forename: R A
authors.initials: RA
authors.lastname: Warren
mesh_terms: D000192:Actinomycetales / Q000201:enzymology / Q000235:genetics*; D002480:Cellulase / Q000235:genetics*; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D007711:Klebsiella pneumoniae / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012244:Rhodospirillaceae / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; D002480:Cellulase