Cloning, sequencing and expression in Escherichia coli of cDNA for a non-A, non-B hepatitis-associated microtubular aggregates protein.
A 1.7 kb cDNA encoding a novel antigen (p44; apparent Mr 44K) associated with non-A, non-B (NANB) hepatitis, was isolated from the hepatic cDNA library of a chimpanzee infected with NANB hepatitis. The library was screened with a monoclonal antibody against this antigen. The cDNA cloned contained an open reading frame encoding a 444 amino acid protein with an Mr calculated to be 50,468. The cDNA hybridized to a 1.9 kb mRNA obtained from chimpanzee hepatocytes infected with either the NANB or hepatitis delta viruses. It hybridized weakly to mRNA from hepatitis B virus-infected hepatocytes, and not at all to mRNA from normal chimpanzee hepatocytes. Southern blot analysis revealed that p44 is a host protein in chimpanzees, and that an identical gene exists in the human genome.
Takahashi
K
K
Biosciences Laboratory, Mitsubishi Kasei Corporation, Yokohama, Japan.
Kitamura
N
N
Shibui
T
T
Kamizono
M
M
Matsui
R
R
Yoshiyama
Y
Y
Maeda
T
T
Kondo
J
J
Honda
Y
Y
Yamada
E
E
D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D003062:Codon / Q000235:genetics; D003598:Cytoskeletal Proteins; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D016174:Hepacivirus / Q000235:genetics*; D006526:Hepatitis C / Q000473:pathology; D008099:Liver / Q000382:microbiology; D008870:Microtubules / Q000648:ultrastructure; D008969:Molecular Sequence Data; D008970:Molecular Weight; D002679:Pan troglodytes; D011487:Protein Conformation; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000302:isolation & purification
D000941:Antigens; D003062:Codon; D003598:Cytoskeletal Proteins; C065888:IFI44 protein, human; D012333:RNA, Messenger; D012367:RNA, Viral; D011994:Recombinant Proteins