[Age-related changes in the activity and post-translational modification of DNA topoisomerase II in the quail oviduct].
In the present work, we show that the activity of DNA topoisomerase II in quail oviduct nuclei significantly increases with age. Posttranslational modifications of the nuclear-matrix-associated enzyme by matrix-bound poly(ADP-ribose) synthetase and protein kinase, whose activities change with age, are demonstrated and offer possible mechanisms by which the age-dependent change in enzyme activity may be caused. It is assumed that the age-correlated enhancement of DNA-topoisomerase-II activity may cause changes in the topological state of DNA, with possible consequences for DNA replication, transcription, and repair with age.
Schröder
H C
HC
Institut für Physiologische Chemie, Mainz.
Steffen
R
R
Ugarković
D
D
Zahn
R K
RK
Müller
W E
WE
D000818:Animals; D002470:Cell Survival / Q000235:genetics*; D003370:Coturnix; D004260:DNA Repair / Q000235:genetics; D004261:DNA Replication / Q000235:genetics; D004250:DNA Topoisomerases, Type II / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D010057:Oviducts / Q000166:cytology; D011065:Poly(ADP-ribose) Polymerases / Q000502:physiology; D011494:Protein Kinases / Q000502:physiology; D011499:Protein Processing, Post-Translational / Q000502:physiology*; D014158:Transcription, Genetic / Q000235:genetics
D011065:Poly(ADP-ribose) Polymerases; D011494:Protein Kinases; D004250:DNA Topoisomerases, Type II
Alternsabhängige Anderungen der Aktivität und posttranslationalen Modifizierung der DNA-Topoisomerase II im Wachtelovidukt.