Construction of human monoclonal antibodies against rabies NS-protein antigen by Epstein-Barr virus transformation.
A human monoclonal antibody against Rabies NS-protein was developed using peripheral blood lymphocytes. A special immunization protocol was used in order to achieve maximal cell numbers of active secreting B cells. These B cells were subjected to Epstein-Barr virus transformation and the transformed cells were cloned stepwise by limiting dilution using supernatant of stimulated macrophages. Active anti-Rabies-IgG secreting cell clones were adapted to serum-free conditions producing stably for more than one year antibodies of the subtype IgG1 at a rate of 10 micrograms/ml/10(6) cells/24 h.
Gebauer
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W
Institut für angewandte Zellkultur, Dr. Toni Lindl GmbH, Munich, Fed. Rep. of Germany.
Lindl
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T
D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D002213:Capsid / Q000276:immunology*; D002472:Cell Transformation, Viral; D002999:Clone Cells; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D004854:Herpesvirus 4, Human; D006801:Humans; D007114:Immunization; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D011820:Rabies virus / Q000276:immunology*; D014758:Viral Core Proteins / Q000276:immunology*; D017361:Viral Nonstructural Proteins
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D007074:Immunoglobulin G; D014758:Viral Core Proteins; D017361:Viral Nonstructural Proteins