title: Solubilization of matrix protein M1/M from virions occurs at different pH for orthomyxo- and paramyxoviruses.
abstract: Enveloped viruses, of which the orthomyxo- and paramyxoviruses are members, are known to be uncoated by nonionic detergents in a salt concentration-dependent manner. In this study we have shown that detergent uncoating of myxoviruses depends not only on salt concentration but also on pH. Treatment of orthomyxoviruses with Nonidet-P40 or Triton N-101 at low salt concentrations results in solubilization of surface virion glycopolypeptides in alkaline and neutral pH (9.0-6.5), but in acidic pH (6.0-5.0) the viral matrix protein M1 is also removed, and the viral ribonucleoprotein complex is released. Conversely, the paramyxovirus matrix protein M is more completely solubilized in alkaline pH (pH 9.0) than in neutral and acidic pH 7.4-5.0. The described pH-dependent differences are discussed in terms of orthomyxo- and paramyxovirus uncoating in target cells.
authors.affiliation: D.I. Ivanovsky Institute of Virology, Moscow, USSR.
authors.forename: O P
authors.initials: OP
authors.lastname: Zhirnov
mesh_terms: D000818:Animals; D002645:Chickens; D006023:Glycoproteins / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D017137:Nonoxynol; D017830:Octoxynol; D009975:Orthomyxoviridae / Q000302:isolation & purification*; D010252:Paramyxoviridae / Q000302:isolation & purification*; D011092:Polyethylene Glycols / Q000494:pharmacology; D012492:Salts / Q000494:pharmacology; D012995:Solubility; D014763:Viral Matrix Proteins / Q000032:analysis*; D014771:Virion / Q000032:analysis* / Q000187:drug effects
chemical_list: D006023:Glycoproteins; D012492:Salts; D014763:Viral Matrix Proteins; C023587:protein M (glycoprotein); D017137:Nonoxynol; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40