Activation of polyphosphoinositide hydrolysis in T cells by H-2 alloantigen but not MLS determinants.
Murine minor lymphocyte-stimulating (Mls) determinants are cell surface antigens that stimulate strong primary T cell responses; the responding T cells display restricted T cell receptor (TCR) V beta gene usage. Interaction of T cells with mitogens or major histocompatibility complex (MHC) antigens activated the polyphosphoinositide (PI) signaling pathway, but this pathway was not triggered by Mls recognition. However, interleukin-2 (IL-2) secretion and proliferation to all three stimuli were comparable. Thus, although recognition of both allo-H-2 and Mls determinants is thought to be mediated by the TCR, these antigens appear to elicit biochemically distinct signal transduction pathways.
O'Rourke
A M
AM
Division of Membrane Biology, Medical Biology Institute, La Jolla, CA 92037.
Mescher
M F
MF
Webb
S R
SR
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D006183:H-2 Antigens / Q000276:immunology*; D006825:Hybridomas; D006868:Hydrolysis; D008213:Lymphocyte Activation; D051379:Mice; D008806:Mice, Inbred AKR; D008808:Mice, Inbred CBA; D016747:Minor Lymphocyte Stimulatory Antigens; D010716:Phosphatidylinositols / Q000378:metabolism*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000378:metabolism*
D000954:Antigens, Surface; D006183:H-2 Antigens; D016747:Minor Lymphocyte Stimulatory Antigens; D010716:Phosphatidylinositols; D011948:Receptors, Antigen, T-Cell