title: The novel inositol lipid phosphatidylinositol 3,4-bisphosphate is produced by human blood platelets upon thrombin stimulation.
abstract: Radioactive PtdIns(3)P was detected in human platelets incubated with [32P]Pi, but remained unaffected by thrombin treatment. In contrast, [32P]PtdIns(3,4)P2 was absent from resting platelets, but was produced by thrombin-activated platelets in a dose- and time-dependent manner. [32P]PtdInsP3 was never found under these conditions. These changes are similar to those elicited in other cells by platelet-derived growth factor or the oncogene product pp60c-src.
authors.affiliation: INSERM Unité 326, Hôpital Purpan, Toulouse, France.
authors.forename: C
authors.initials: C
authors.lastname: Sultan
authors.forename: M
authors.initials: M
authors.lastname: Breton
authors.forename: G
authors.initials: G
authors.lastname: Mauco
authors.forename: P
authors.initials: P
authors.lastname: Grondin
authors.forename: M
authors.initials: M
authors.lastname: Plantavid
authors.forename: H
authors.initials: H
authors.lastname: Chap
mesh_terms: D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008055:Lipids / Q000096:biosynthesis / Q000097:blood; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols / Q000097:blood*; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D013268:Stimulation, Chemical; D013917:Thrombin / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D008055:Lipids; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; D010761:Phosphorus Radioisotopes; C060980:phosphatidylinositol 3,4-diphosphate; D013917:Thrombin