A novel transcriptional regulatory factor that binds to the polyoma virus enhancer in a developmental stage-specific manner.
By using gel mobility shift assay, it was shown that the nuclear extract from F9 embryonal carcinoma (EC) cells contains a novel transcriptional regulatory factor, BF-H, that binds to the 5' upstream region of the early gene of polyoma virus. Two binding sites were located in the transcriptional enhancer domain "A" (nucleotide 5034-5041) and in the 5' upstream of the domain "A" (4998-5005), having a consensus motif (AAPuATGG) between them. Combination of in vitro mutagenesis with chloramphenicol acetyltransferase (CAT) assay revealed that BF-H is a positive transcriptional factor. Interestingly, the binding of BF-H disappeared after differentiation of F9 cells by treatment with retinoic acid, whereas BF-H was present in the F9 cells differentiated with both retinoic acid and dibutyryl cyclic AMP (dbcAMP). These observations suggest that BF-H regulates the expression of genes in a developmental stage-specific manner in early embryos of the mouse.
Hirano
T
T
Institute of Medical Science, University of Tokyo, Japan.
Iwakura
Y
Y
D017456:Adenovirus Early Proteins; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002454:Cell Differentiation; D004742:Enhancer Elements, Genetic; D006801:Humans; D008745:Methylation; D051379:Mice; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009856:Oncogene Proteins, Viral / Q000235:genetics; D011120:Polyomavirus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D017456:Adenovirus Early Proteins; D009856:Oncogene Proteins, Viral; D014157:Transcription Factors; D014212:Tretinoin