Identification of Pneumocystis carinii chromosomes and mapping of five genes.
Pulsed field gel electrophoresis was used to identify the chromosome-size DNA of Pneumocystis carinii, a major pathogen of immunocompromised patients. Thirteen chromosomes of rodent Pneumocystis carinii, ranging in size from 300 to 700 kilobases (kb), were identified. The minimum genome size for P. carinii, estimated on the basis of the sizes of chromosomes, is 7,000 kb. Genetic heterogeneity among different P. carinii isolates was documented by demonstration of chromosomal size variability. By hybridization studies, the genes for topoisomerase I, dihydrofolate reductase, rRNA, actin, and thymidylate synthase were mapped to single chromosomes of approximately 650, 590, 550, 460, and 350 kb, respectively. Hybridization studies further confirmed the genetic heterogeneity of P. carinii.
Lundgren
B
B
Critical Care Medicine, National Institutes of Health, National Institute of Alcohol Abuse and Alcoholism, Bethesda, Maryland 20892.
Cotton
R
R
Lundgren
J D
JD
Edman
J C
JC
Kovacs
J A
JA
D000199:Actins / Q000235:genetics; D015139:Blotting, Southern; D002874:Chromosome Mapping; D004264:DNA Topoisomerases, Type I / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005800:Genes, Fungal; D011010:Pneumocystis / Q000235:genetics*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D015183:Restriction Mapping; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics; D013940:Thymidylate Synthase / Q000235:genetics
D000199:Actins; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D013762:Tetrahydrofolate Dehydrogenase; D013940:Thymidylate Synthase; D004264:DNA Topoisomerases, Type I