Regional variation of biochemical characteristics and antigeneity in Great Basin rattlesnake (Crotalus viridis lutosus) venom.
1. Three pooled and 20 individual venom samples of Crotalus viridis lutosus from different localities in Utah and Arizona were screened and fractionated with HPLC-anion exchange. 2. Pooled venom samples and fractions were tested for hemorrhagic, collagenase, and phospholipase activities, and reactivity to a monoclonal antibody against a hemorrhagin from C. atrox venom (CA-P-8) using ELISA. 3. The 20 individual samples were organized into four groups based on their HPLC profiles. 4. ELISA results and specific hemorrhagic activity of the venom samples displayed a variation in latitidinal distribution although from the same species.
Adame
B L
BL
Department of Biology, Texas A&I University, Kingsville 78363.
Soto
J G
JG
Secraw
D J
DJ
Perez
J C
JC
Glenn
J L
JL
Straight
R C
RC
D000818:Animals; D000911:Antibodies, Monoclonal; D002851:Chromatography, High Pressure Liquid; D003435:Crotalid Venoms / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D010450:Endopeptidases / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D014644:Genetic Variation; D003012:Microbial Collagenase / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism; D012911:Snakes
D000911:Antibodies, Monoclonal; D003435:Crotalid Venoms; D010740:Phospholipases; D010450:Endopeptidases; C041605:hemorrhagic proteinase IV; D003012:Microbial Collagenase