Retinoic acid and epidermal growth factor binding in retinoid-mediated invasion suppressed human lung carcinoma cells.
The effect of retinoic acid (RA)-induced suppression of in vitro invasive ability of A549 human lung carcinoma cells on cellular binding of RA and epidermal growth factor (EGF) was investigated. RA inhibition of cell invasive potential was accompanied by a significant increase in specific high affinity cellular retinoic acid binding protein (CRABP) level. An approximately 2.7-fold increase in cytosolic CRABP was found in RA-treated cells (450 fm/mg protein compared to 167 fm/mg control cell protein). In contrast, 125I-EGF ligand binding was similar for control and treated cells.
Fazely
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F
Dana-Farber Cancer Research Institute, Boston, MA 02115.
Ledinko
N
N
D002731:4-Chloromercuribenzenesulfonate / Q000494:pharmacology; D002352:Carrier Proteins / Q000378:metabolism; D002460:Cell Line; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008175:Lung Neoplasms / Q000473:pathology; D009361:Neoplasm Invasiveness; D018168:Receptors, Retinoic Acid; D014212:Tretinoin / Q000378:metabolism / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
D002352:Carrier Proteins; D018168:Receptors, Retinoic Acid; D014212:Tretinoin; D002731:4-Chloromercuribenzenesulfonate; D004815:Epidermal Growth Factor; D066246:ErbB Receptors