Immunization with baculovirus-expressed VP4 protein passively protects against simian and murine rotavirus challenge.
A baculovirus-expressed VP4 protein derived from the simian rhesus rotavirus (RRV) was used to parenterally immunize murine dams. VP4-immunized dams developed high levels of neutralizing antibodies against RRV and low levels of cross-reactive neutralizing antibodies against human strains Wa, ST3, and S2 and animal strains SA-11, NCDV, and Eb. Newborn mice suckled on VP4-immunized dams were protected against a virulent challenge dose of the simian strain RRV and against murine rotavirus Eb. The cross-reactive nature of the serum-neutralizing response generated by VP4 immunization and the protective efficacy of the immunization suggest that recombinant-expressed VP4 proteins should be considered as viable vaccine candidates.
Mackow
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Department of Microbiology and Immunology, Stanford University, California 94305.
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Broome
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Bass
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Greenberg
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HB
D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000276:immunology; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D005260:Female; D006385:Hemagglutination Inhibition Tests; D008253:Macaca mulatta / Q000382:microbiology; D051379:Mice; D009500:Neutralization Tests; D011233:Precipitin Tests; D012401:Rotavirus / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus; D014614:Vaccines, Synthetic; D014765:Viral Vaccines