A minor microtubule-associated protein is responsible for the stimulation of vesicular stomatitis virus transcription in vitro.
An activity found in bovine brain microtubule-associated proteins (MAPs) which stimulated vesicular stomatitis virus (VSV) transcription in vitro co-eluted from a gel filtration column (Sepharose CL-6B) with a minor MAP subset of an apparent Mr of 100,000 to 250,000. The activity did not appear to be closely associated with MAPs 1, MAPs 2, tau or tubulin. Anti-MAPs 1 and anti-MAPs 2 IgG did not reduce or abolish the stimulatory activity. The bovine brain MAPs stimulatory activity was similar to that found in HeLa cell extracts: both were heat-resistant, and both co-purified with the MAPs fraction of cell extracts; also amounts of each which gave maximum stimulation when used separately did not give additional stimulation when combined. Fractions from the Sepharose CL-6B column that contained the greatest amount of stimulatory activity exhibited little or no cAMP-dependent or -independent kinase activity. The MAPs stimulatory activity required the presence of both L and NS polymerase subunits.
Hill
V M
VM
Department of Cellular, Viral and Molecular Biology, University of Utah School of Medicine, Salt Lake City 84132.
Summers
D F
DF
D000818:Animals; D001345:Autoradiography; D001923:Brain Chemistry; D002417:Cattle; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D013698:Templates, Genetic; D014158:Transcription, Genetic; D014404:Tubulin / Q000235:genetics; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics*
D008869:Microtubule-Associated Proteins; D014404:Tubulin; D011494:Protein Kinases