Regulation of ion conductance in frog skin by isoproterenol.
The effect of isoproterenol on apical and basolateral membrane conductance in principal cells of short-circuited frog skin was analyzed using microelectrodes. Isoproterenol (10(-6) mol/l) increased the apical membrane conductance in addition to stimulating Cl- conductive pathways outside the principal cells. The effect on apical Na+ channels explains the increase in amiloride sensitive short-circuit current. Basolateral membrane conductance increased only slightly. Steady-state I/V relationships of the basolateral membrane indicate that the inward rectification of basolateral membrane K+ channels was not altered.
Granitzer
M
M
Département de Physiologie, Faculté de Médecine, U.C.L., Bruxelles, Belgium.
Nagel
W
W
Crabbé
J
J
D000584:Amiloride; D000818:Animals; D001001:Anura; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D007545:Isoproterenol / Q000494:pharmacology*; D007700:Kinetics; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism*; D012867:Skin / Q000187:drug effects / Q000378:metabolism*; D015222:Sodium Channels / Q000187:drug effects / Q000378:metabolism*
D015221:Potassium Channels; D015222:Sodium Channels; D000584:Amiloride; D007545:Isoproterenol