title: In vitro protein translocation into inverted membrane vesicles prepared from Vibrio alginolyticus is stimulated by the electrochemical potential of Na+ in the presence of Escherichia coli SecA.
abstract: A protein translocation system was reconstituted from inverted membrane vesicles prepared from Na+ pump-possessing Vibrio alginolyticus and purified Escherichia coli SecA. The translocation required ATP and was stimulated by the functioning of the Na+ pump, suggesting that the electrochemical potential of Na+, but not that of H+, is important for protein translocation in Vibrio.
authors.affiliation: Institute of Applied Microbiology, University of Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tokuda
authors.forename: Y J
authors.initials: YJ
authors.lastname: Kim
authors.forename: S
authors.initials: S
authors.lastname: Mizushima
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D001693:Biological Transport, Active; D002462:Cell Membrane / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D007700:Kinetics; D009154:Mutation; D012964:Sodium / Q000378:metabolism*; D014733:Vibrio / Q000235:genetics / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D012964:Sodium