[Initial experience with the use of monoclonal antibodies in the detection of cytomegalovirus early antigens in patients with transplants].
10 diabetics with kidney and pancreas transplantation (6) and with isolated kidney transplantation (4) underwent examination with the aim to produce evidence of cytomegalovirus excretion (CMV) by the urine. To prove the presence of CMV antigen, the authors used monoclonal antibody produced by DuPont. CMV was found to be present in the urine of 4 patients. The examination lasts max. 48 hrs. It yielded no evidence of interdependence between viruria and the serum levels of the CMV antibodies. Tests for CMV in biological material by means of monoclonal antibody is a modern and quick method which, unlike conventional virological procedures (serology, virus cultivation), plays an important part in clinical medicine.
Brůcek
P
P
I. interní výzkumná základna, Institutu klinické a experimentální medicíny, Praha.
Otová
B
B
Horácek
J
J
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis*; D003587:Cytomegalovirus / Q000276:immunology*; D006801:Humans; D017874:Immediate-Early Proteins; D016030:Kidney Transplantation / Q000276:immunology*; D016035:Pancreas Transplantation / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D017874:Immediate-Early Proteins; C044582:immediate-early proteins, cytomegalovirus
První zkusenosti s vyuzitím monoklonální protilátky k detekci casného cytomegalovirového antigenu u transplantovaných nemocných.