[The role of interleukin-1 beta in the regulation of liver-specific gene expression in human hepatoblastoma cells].
The authors studied the function of liver cells--on level of DNA and gene regulation--by methods of molecular biology. They found that treating human hepatoma (Hep G2) cells with interleukin-1 (IL-1) leads to the induction of alpha-1-acidglycoprotein (AGP) and complement 3 (C3) mRNA synthesis, and to a concomitant downregulation of albumin (alb), alpha-fetoprotein (AFP) and alpha-2-macroglobulin (alpha 2M) mRNA synthesis. Levels of specific mRNA were measured by Northern-blot analysis. They conclude that Hep G2 cells may serve as a suitable in vitro model for study of the liver specific gene expression, and IL-1 is one of the mediators of these gene control. The regulation is pretranslational as the direction of change in specific mRNA corresponds to the changes in synthesis of the respective proteins.
Lengyel
G
G
Semmelweis Orvostudományi Egyetem, II. sz. Belklinika, Budapest.
Joachim
B
B
Acs
G
G
Fehér
J
J
D015152:Blotting, Northern; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000648:ultrastructure; D015870:Gene Expression; D005809:Genes, Regulator; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D008113:Liver Neoplasms / Q000235:genetics* / Q000648:ultrastructure; D009363:Neoplasm Proteins / Q000096:biosynthesis; D012333:RNA, Messenger
D007375:Interleukin-1; D009363:Neoplasm Proteins; D012333:RNA, Messenger
Az interleukin-1 béta szerepe a májspecifikus génexpressziók szabályozásában humán hepatoblastoma sejtekben.