Use of gel filtration in the preparation of biological fluids for magnetic resonance spectroscopy.
Analysis of biological fluids by proton magnetic resonance spectroscopy is often complicated by dynamic range problems created from the large water resonance. Gel filtration chromatography is found to be a simple and nondestructive method for exchanging D2O for H2O and for removing low molecular weight molecules from both plasma and urine, significantly improving subsequent one- and two-dimensional MRS spectra.
Hoffman
D W
DW
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710.
Venters
R A
RA
Shedd
S F
SF
Spicer
L D
LD
D001834:Body Water / Q000032:analysis* / Q000502:physiology; D002850:Chromatography, Gel; D006801:Humans; D008055:Lipids / Q000032:analysis* / Q000097:blood; D009682:Magnetic Resonance Spectroscopy; D011522:Protons; D014556:Urine / Q000032:analysis*
D008055:Lipids; D011522:Protons