Pyrophosphate-loaded hepatocytes show increased iron uptake from transferrin.
Exogenously added pyrophosphate was found associated with hepatocytes in suspension, particularly in the presence of calcium ions. The cell-associated pyrophosphate was recovered with the stroma fraction. Pyrophosphate thus bound did not affect cellular ATP, leakage of lactate dehydrogenase or trypan blue exclusion. Compared to control cells, pyrophosphate-loaded hepatocytes showed an approximately 70% increase in iron accumulation from transferrin. The excess iron was handled by the pyrophosphate-loaded cells as control cells. The relevance of pyrophosphate to hepatocyte iron uptake is discussed.
Nilsen
T
T
Department of Clinical Chemistry, University of Trondheim, Norway.
Romslo
I
I
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D011756:Diphosphates / Q000378:metabolism / Q000494:pharmacology*; D007501:Iron / Q000378:metabolism*; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D014168:Transferrin / Q000378:metabolism*; D014343:Trypan Blue / Q000378:metabolism
D011756:Diphosphates; D014168:Transferrin; D000255:Adenosine Triphosphate; D007501:Iron; D007770:L-Lactate Dehydrogenase; D014343:Trypan Blue; D002118:Calcium