title: Intracellular protein phosphorylation in oat (Avena sativa L.) protoplasts by phytochrome action: involvement of protein kinase C.
abstract: Phosphorylations of two proteins (27 KDa, 32 KDa) in oat cells were dependent on phytochrome action. To determine which kinase system(s) for the phosphorylation of these two proteins are controlled by the phytochrome, involvement of the Ca2+/DG dependent protein kinase (protein kinase C) was first investigated. When a protein kinase C inhibitor (1-(5-isoquinoline sulfonyl)-2-methylpiperazine:H-7) or the inositol phospholipid metabolic blocker Li+ was added into the cell suspension, respectively, the phosphorylations of these two proteins were substantially reduced. On the other hand, an addition of 1-oleoyl-2-acetyl-sn-glycerol (OAG:activator of protein kinase C) or phorbol 12-myristate 13-acetate (TPA: tumor promoting phorbol ester) enhanced the phosphorylations of these proteins. These results suggest that phytochrome action is certainly connected with the protein phosphorylation via the activation of protein kinase C or a similar molecule with protein kinase C.
authors.affiliation: Department of Biochemistry, Chungbuk National University, Republic of Korea.
authors.forename: M H
authors.initials: MH
authors.lastname: Park
authors.forename: Q
authors.initials: Q
authors.lastname: Chae
mesh_terms: D002118:Calcium / Q000502:physiology; D004075:Diglycerides / Q000494:pharmacology; D002523:Edible Grain / Q000378:metabolism*; D008094:Lithium / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000037:antagonists & inhibitors; D010834:Phytochrome / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*
chemical_list: D004075:Diglycerides; D010716:Phosphatidylinositols; D010750:Phosphoproteins; D010940:Plant Proteins; D010834:Phytochrome; D008094:Lithium; D011493:Protein Kinase C; D010744:Phosphoric Monoester Hydrolases; C021822:myo-inositol-1 (or 4)-monophosphatase; D002118:Calcium