Properties of new Escherichia coli Hfr strains constructed by integration of pSC101-derived conjugative plasmids.
Conjugative temperature-sensitive plasmids were derived from pSC101. These plasmids are useful in genetic analysis for two reasons: (i) they render possible the construction of new Hfr lines by plasmid integration at predetermined chromosomal loci via Tn10 inverse transposition, and (ii) the Hfr characters are transducible via bacteriophage P1. We also showed that replication from pSC101 origin is deleterious for the plasmid-chromosome fusion.
François
V
V
Centre de Biochimie et de Génétique cellulaires du Centre National de la Recherche Scientifique, Toulouse, France.
Conter
A
A
Louarn
J M
JM
D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D005838:Genotype; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D013696:Temperature
D004251:DNA Transposable Elements