Transformation of murine bone marrow cells with combined v-raf-v-myc oncogenes yields clonally related mature B cells and macrophages.
Murine bone marrow cells infected with replication-defective retroviruses containing v-raf alone or v-myc alone yielded transformed pre-B cell lines, while a retroviral construct containing both v-raf and v-myc oncogenes produced clonally related populations of mature B cells and mature macrophages. The genealogy of these transformants demonstrates that mature myeloid cells were derived from cells with apparent B-lineage commitment and functional immunoglobulin rearrangements. This system should facilitate studies of developmental relationships in hematopoietic differentiation and analysis of lineage determination.
Principato
M
M
Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
Cleveland
J L
JL
Rapp
U R
UR
Holmes
K L
KL
Pierce
J H
JH
Morse
H C
HC
Klinken
S P
SP
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D001854:Bone Marrow Cells; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D015321:Gene Rearrangement; D006378:Helper Viruses / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D008815:Mice, Inbred Strains; D008979:Moloney murine leukemia virus / Q000235:genetics; D027384:Oncogene Proteins v-raf; D009857:Oncogenes; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics*; D009053:Sarcoma Viruses, Murine / Q000235:genetics
D007147:Immunoglobulin Light Chains; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D015682:Retroviridae Proteins, Oncogenic; D011505:Protein-Tyrosine Kinases; D027384:Oncogene Proteins v-raf