title: Identification and structural analysis of a ribosomal RNA gene promoter from Thiobacillus ferrooxidans.
abstract: The 5'-terminus of a rRNA operon (rrnT2) from Thiobacillus ferrooxidans was characterized. The rRNA promoters from this microorganism were identified by means of a functional assay in Escherichia coli. DNA sequencing of the promoter region, upstream the 16 S rRNA gene, showed the presence of a consensus sequence for bacterial ribosomal promoters. Other features such as a 'discriminator' sequence, antiterminator elements and an upstream hexanucleotide common to several rRNA operons were also found. Two other putative transcription promoters were also identified.
authors.affiliation: Departamento de Bioquimica, Facultad de Medicina, Universidad de Chile, Santiago.
authors.forename: M
authors.initials: M
authors.lastname: Takamiya
authors.forename: O
authors.initials: O
authors.lastname: Salazar
authors.forename: D
authors.initials: D
authors.lastname: Vargas
authors.forename: E
authors.initials: E
authors.lastname: Jedlicki
authors.forename: O
authors.initials: O
authors.lastname: Orellana
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009876:Operon; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012335:RNA, Ribosomal / Q000737:chemistry / Q000235:genetics*; D013855:Thiobacillus / Q000235:genetics*; D014158:Transcription, Genetic; D014169:Transformation, Bacterial
chemical_list: D004269:DNA, Bacterial; D012335:RNA, Ribosomal; D004262:DNA Restriction Enzymes