L-methionine-DL-sulfoximine induces massive efflux of glutamine from cortical astrocytes in primary culture.
L-Methionine-DL-sulfoximine (MSO) is a potent convulsant which metabolically and morphologically primarily affects astroglia, and the links between the gliotoxic and neurophysiological effects of MSO are not clear. A 5 min treatment with 3 mM MSO increased the rate of efflux of newly loaded radiolabelled glutamine (Gln) from rat cortical astrocytes in primary culture to more than 400% of the basal efflux. MSO did not affect the efflux of the neurotransmitter amino acids GABA or D-aspartate (a non-metabolizable analogue of L-glutamate), under the same experimental conditions. MSO-induced overflow with Gln, which according to a recent account strongly interacts with the N-methyl-D-aspartate (NMDA) receptor complex, may contribute to the convulsive action of the drug.
Albrecht
J
J
Veteran Administration Medical Center, Department of Pathology, Miami, FL.
Norenberg
M D
MD
D000818:Animals; D000831:Animals, Newborn; D001224:Aspartic Acid / Q000378:metabolism; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism*; D008717:Methionine Sulfoximine / Q000494:pharmacology*; D051381:Rats; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D005973:Glutamine; D008717:Methionine Sulfoximine; D001224:Aspartic Acid; D005680:gamma-Aminobutyric Acid; D003994:Bucladesine