[Determination of intracellular concentration of H+, Na+, K+ and ATP in Bacteroides succinogenes adapted to monensin].
The internal concentrations of the cations H+, Na+, K+ and the intracellular ATP concentration were measured on B succinogenes adapted to the presence of the ionophore monensin (0.5 microM). Adapted bacteria were still able to regulate the intracellular concentrations of Na+ and K+, but their internal pH and ATP concentration were lower than in control bacteria.
Forano
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Laboratoire de Microbiologie, INRA, Theix, Ceyrat, France.
D000255:Adenosine Triphosphate / Q000032:analysis*; D001439:Bacteroides / Q000032:analysis* / Q000378:metabolism; D004352:Drug Resistance, Microbial; D006863:Hydrogen-Ion Concentration; D008985:Monensin / Q000378:metabolism*; D011188:Potassium / Q000032:analysis*; D011522:Protons; D012964:Sodium / Q000032:analysis*
D011522:Protons; D000255:Adenosine Triphosphate; D008985:Monensin; D012964:Sodium; D011188:Potassium
Mesure des concentrations intracellulaires en H+, Na+, K+ et ATP chez Bacteroides succinogenes adaptée à la monensine.