Pancreastatin producing cell line from human pancreatic islet cell tumor.
It has been characterized that cell line QGP-1 derived from human non-functioning pancreatic islet cell tumor produces human pancreastatin. Exponentially growing cultures produced 5.7 fmol of pancreastatin/10(6) cells/hr. Human pancreastatin immunoreactivities in plasma and tumor after xenografting with QGP-1 into nude mouse were 92.7 fmol/ml and 160.2 pmol/g wet weight, respectively. Immunocytochemical study revealed both chromogranin A and pancreastatin immunoreactive cells in the tumor. Gel filtrations of culture medium and tumor extract identified heterogenous molecular forms of PST-LI which eluted as large and smaller molecular species. These results suggest that plasma pancreastatin levels may be useful as a tumor marker of endocrine tumor of the pancreas, and the pancreastatin producing cell line may be useful for studies of the mechanism of secretions and processing of chromogranin A and pancreastatin.
Funakoshi
A
A
Department of Gastroenterology, National Kyushu Cancer Center, Fukuoka, Japan.
Tateishi
K
K
Tsuru
M
M
Jimi
A
A
Wakasugi
H
H
Ikeda
Y
Y
Kono
A
A
D007516:Adenoma, Islet Cell / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D053379:Chromogranin A; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D010187:Pancreatic Hormones / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D011863:Radioimmunoassay
D053379:Chromogranin A; D010187:Pancreatic Hormones; C050836:pancreastatin