Suppressive effects of intracellular glutathione on hydroxyl radical production induced by tumor necrosis factor.
Protective effects of intracellular glutathione (GSH) against the cytotoxicity of human recombinant tumor necrosis factor (TNF) were investigated. Three tumor cell lines (L-M, B-16, HeLa) were used as target cells. Exposure of these cells to buthionine sulfoximine (BSO) or diethyl maleate (DEM) resulted in the depletion of intracellular GSH content to 5.2-43.0% of control values and enhancement of their susceptibility to TNF cytotoxicity. The hydroxyl radical production in L-M cells stimulated by TNF was increased by treatment with BSO or DEM. These results are consistent with the suggestion that intracellular GSH exerts its protective function against the cytocidal effect of TNF by inhibiting the hydroxyl radical production stimulated by TNF.
Yamauchi
N
N
Department of Internal Medicine, Sapporo Medical College, Japan.
Watanabe
N
N
Kuriyama
H
H
Neda
H
H
Maeda
M
M
Himeno
T
T
Tsuji
Y
Y
Niitsu
Y
Y
D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D019328:Buthionine Sulfoximine; D002417:Cattle; D005978:Glutathione / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D006878:Hydroxides / Q000378:metabolism*; D017665:Hydroxyl Radical; D007424:Intracellular Fluid / Q000378:metabolism*; D008298:Maleates / Q000494:pharmacology; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives / Q000494:pharmacology; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology* / Q000633:toxicity
D000963:Antimetabolites; D006878:Hydroxides; D008298:Maleates; D014409:Tumor Necrosis Factor-alpha; D008717:Methionine Sulfoximine; D017665:Hydroxyl Radical; D019328:Buthionine Sulfoximine; C014476:diethyl maleate; D005978:Glutathione