Two distinct types of Fc receptor for IgG on guinea pig macrophages activate the NADPH oxidase through different signal-transduction pathways.
Neither 5 microM calmodulin antagonist W-7 nor depletion of the intracellular Ca2+ affected the O2- generation triggered by the Fc receptor for IgG2 (Fc gamma 2 R) on guinea pig macrophages, although that by the Fc receptor for both IgG1 and IgG2 (Fc gamma 1/gamma 2 R) was lowered to 50% of a control level by W-7 and the Ca2+ depletion, respectively. Staurosporine inhibited all these responses, but to different extents. These results reveal that the signal-transduction pathways linked to these receptors differ from each other, and that Fc gamma 1/gamma 2 R triggers the O2- generation through the Ca2(+)-calmodulin system and the Ca2(+)-independent system.
Imamichi
T
T
Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Koyama
J
J
D000818:Animals; D000943:Antigens, Differentiation / Q000502:physiology*; D002147:Calmodulin / Q000037:antagonists & inhibitors; D006168:Guinea Pigs; D007074:Immunoglobulin G / Q000276:immunology; D066298:In Vitro Techniques; D007700:Kinetics; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D019255:NADPH Oxidases; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D011961:Receptors, Fc / Q000502:physiology*; D017452:Receptors, IgG; D015398:Signal Transduction; D013449:Sulfonamides / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000943:Antigens, Differentiation; D002147:Calmodulin; D007074:Immunoglobulin G; D011961:Receptors, Fc; D017452:Receptors, IgG; D013449:Sulfonamides; D013481:Superoxides; C017967:W 7; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate