Preparation of nucleate and anucleate fragments of hamster and mouse eggs by centrifugation.
Nucleate and anucleate fragments of hamster and mouse eggs can be prepared by centrifuging zona-free eggs in a Percoll gradient. These fragments are fertilizable and can be used for various studies of nucleus-ooplasm interactions.
Yanagimachi
R
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Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu 96822.
Yang
C H
CH
D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002467:Cell Nucleus; D002499:Centrifugation, Density Gradient; D006224:Cricetinae; D015638:Cytochalasin D; D005260:Female; D005306:Fertilization; D008647:Mesocricetus; D051379:Mice; D010063:Ovum / Q000648:ultrastructure*; D011205:Povidone; D012822:Silicon Dioxide
D015638:Cytochalasin D; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone