Expression of phosphoglycerate mutase mRNA in differentiating rat satellite cell cultures.
Poly(A)+ mRNA was isolated from rat satellite cell cultures and analyzed by Northern blot analyses for mRNA content of phosphoglycerate mutase (PGAM) isozymes. In non-differentiating satellite cells only PGAM-B mRNA was detected, but when cells were differentiated into myotubes, which undergo spontaneous contraction, mRNA for PGAM-M muscle-specific isozyme was also detected. This finding is in perfect concordance with the transition of PGAM isozymes encountered in the same cell cultures, and strongly supports a transcriptional control of PGAM expression throughout myogenesis independently of nerve influence.
Castellà-Escolà
J
J
Unitat de Bioquímica, Facultat de Medicina, Universitat de Barcelona, Spain.
Ureña
J
J
Alterio
J
J
Carreras
J
J
Martelly
I
I
Climent
F
F
D000818:Animals; D001731:Bisphosphoglycerate Mutase / Q000235:genetics*; D015152:Blotting, Northern; D002454:Cell Differentiation; D002478:Cells, Cultured; D015971:Gene Expression Regulation, Enzymologic; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000378:metabolism; D009132:Muscles / Q000166:cytology* / Q000201:enzymology; D010770:Phosphotransferases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D014158:Transcription, Genetic
D007527:Isoenzymes; D012333:RNA, Messenger; D010770:Phosphotransferases; D001731:Bisphosphoglycerate Mutase