Luminol-amplified chemiluminescence activity in human monocytes: a comparison with the activity induced in granulocytes.
The characteristics of the luminol-amplified chemiluminescence (CL) induced in mononuclear phagocytes interacting with PMA, FMLP or ionomycin were determined. Azide reduced the CL activity by more than 80%, while superoxide dismutase and catalase had minor effect on the monocyte CL response. The sensitivity of the monocyte CL response, to the addition of extra peroxidase differed depending on the stimulus used. Furthermore, no direct correlation was obtained between the CL response and superoxide anion or hydrogen peroxide production. In comparison with the response in granulocytes, minor quantitative differences were observed. The mechanism for the light-generating reaction, seems to be the same in both cell types.
Johansson
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A
Department of Medical Microbiology, University of Linköping, Sweden.
Dahlgren
C
C
D000328:Adult; D006098:Granulocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D066298:In Vitro Techniques; D015759:Ionomycin / Q000494:pharmacology; D008163:Luminescent Measurements; D008165:Luminol; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D010544:Peroxidases / Q000378:metabolism; D011724:Pyridazines; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D011724:Pyridazines; D013481:Superoxides; D015759:Ionomycin; D009240:N-Formylmethionine Leucyl-Phenylalanine; D008165:Luminol; D006861:Hydrogen Peroxide; D010544:Peroxidases; D013755:Tetradecanoylphorbol Acetate