Evi-2, a common integration site involved in murine myeloid leukemogenesis.
BXH-2 mice have the highest incidence of spontaneous retrovirally induced myeloid leukemia of any known inbred strain and, as such, represent a valuable model system for identifying cellular proto-oncogenes involved in myeloid disease. Chronic murine leukemia viruses often induce disease by insertional activation or mutation of cellular proto-oncogenes. These loci are identified as common viral integration sites in tumor DNAs. Here we report on the characterization of a novel common viral integration site in BXH-2 myeloid leukemias, designated Evi-2. Within the cluster of viral integration sites that define Evi-2, we identified a gene that has the potential for encoding a novel protein of 223 amino acids. This putative proto-oncogene possesses all of the structural features of a transmembrane protein. Within the transmembrane domain is a "leucine zipper," suggesting that Evi-2 is involved in either homopolymer or heteropolymer formation, which may play an important role in the normal functioning of Evi-2. Interestingly, the human homolog of Evi-2 has recently been shown to be tightly linked to the von Recklinghausen neurofibromatosis locus, suggesting a role for Evi-2 in human disease as well.
Buchberg
A M
AM
Mammalian Genetics Laboratory, NCI-Frederick Cancer Research and Development Center, Frederick, Maryland 21702.
Bedigian
H G
HG
Jenkins
N A
NA
Copeland
N G
NG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D015967:Gene Expression Regulation, Viral; D015723:Gene Library; D007256:Information Systems; D009052:Leukemia Virus, Murine / Q000235:genetics* / Q000302:isolation & purification; D007942:Leukemia, Experimental / Q000235:genetics* / Q000382:microbiology; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D011487:Protein Conformation; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes; D011995:Recombination, Genetic; D015183:Restriction Mapping; D012984:Software; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics
D015342:DNA Probes; D004279:DNA, Viral; C065457:Evi2a protein, mouse; C000715470:MAS1 protein, human; D008565:Membrane Proteins; D000090063:Proto-Oncogene Mas; D014764:Viral Proteins