The mutation of V79 cells by N-nitrosobis(2-oxopropyl)amine activated by pancreas acinar and duct tissue from Syrian hamsters and MRC-Wistar rats.
The mutagenicity of N-nitrosobis(2-oxopropyl)amine was measured in the V79 assay using homogenates of acinar cells and duct tissue from the pancreases of Syrian hamsters and MRC-Wistar rats as the activating systems. Mutations at the sodium/potassium ATPase and hypoxanthine:guanine phosphoribosyltransferase loci were measured by resistance to ouabain and 6-thioguanine (TG). The order of effectiveness in generating mutagens from BOP was hamster duct, hamster acinar, rat duct, rat acinar. These data show extensive differences in BOP activation by hamster acinar and duct tissue.
Lawson
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T
Eppley Institute for Research in Cancer, University of Nebraska Medical Center, Omaha 68105-1065.
Kolar
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C
Nagel
D
D
D000818:Animals; D001711:Biotransformation; D006224:Cricetinae; D004351:Drug Resistance / Q000235:genetics; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics / Q000378:metabolism; D008297:Male; D008647:Mesocricetus; D009152:Mutagenicity Tests; D009154:Mutation; D009602:Nitrosamines / Q000493:pharmacokinetics / Q000633:toxicity*; D010042:Ouabain / Q000494:pharmacology; D010179:Pancreas / Q000166:cytology / Q000378:metabolism*; D010183:Pancreatic Ducts / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000378:metabolism; D013866:Thioguanine / Q000494:pharmacology
D009602:Nitrosamines; D010042:Ouabain; C013622:nitrosobis(2-oxopropyl)amine; D007041:Hypoxanthine Phosphoribosyltransferase; D000254:Sodium-Potassium-Exchanging ATPase; D013866:Thioguanine