Active and pro-plasminogen activator on the surface of human bladder cancer cells derived from a high grade invasive tumour.
Intact UCRU-BL28 cells derived from a high grade invasive human bladder cancer produced both active and pro-plasminogen activator. Following culture of cells in the presence of plasminogen, all of the pro-plasminogen activator was converted to an active form. Cell-surface extracts showed plasminogen activator activity with a molecular mass of 55,000, similar to that of human urokinase. Extracts also contained gelatinase activities with molecular masses of 101,000; 80,000; 74,000 and 70,000 in polyacrylamide gel electrophoresis. These tumour cell-surface proteinases may be involved in invasion and metastasis in human bladder cancer.
Grant
A J
AJ
Urological Cancer Research Unit, Royal Prince Alfred Hospital, N.S.W., Australia.
Russell
P J
PJ
Raghavan
D
D
D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D004792:Enzyme Precursors / Q000302:isolation & purification* / Q000378:metabolism; D018093:Gelatinases; D006801:Humans; D007527:Isoenzymes / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D009361:Neoplasm Invasiveness; D010434:Pepsin A / Q000302:isolation & purification / Q000378:metabolism; D010960:Plasminogen Activators / Q000302:isolation & purification* / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000201:enzymology* / Q000473:pathology
D004792:Enzyme Precursors; D007527:Isoenzymes; D010960:Plasminogen Activators; D010434:Pepsin A; D018093:Gelatinases