A comparison of cervical scrapes for HPV typing by dot-blot hybridization obtained by wood and plastic spatulas.
A cross-over study was designed to determine whether the type of spatula used to collect cervical cells influences the ability of dot-blot hybridization to detect HPV DNA. Fifty-nine patients had a cervical scrape with a wood spatula first and a plastic spatula second: 60 were scraped in the inverse order. The order of sampling did not affect the HPV DNA positivity rate, which was nearly similar for both wood and plastic spatulas (30 and 32%, respectively). Wood spatulas collected more cells and greater than 1 x 10(5) cells more often than plastic spatulas (P = 0.001 and 0.06, respectively). Non-purple (negative) dots were more frequent in samples obtained by wood than by plastic spatulas (P = 0.001). The study showed that cervical cell collection by wood spatulas is preferred as they harvest more cells, thus optimizing the sensitivity of the hybridization method, and the spatulas are also more economical. Although they yielded more non-purple dots, a reduction in these dots by using plastic spatulas did not result in a significantly increased HPV positivity rate.
Duggan
M A
MA
Department of Pathology, Foothills Hospital, Tom Baker Cancer Centre, Calgary, Alberta, Canada.
Inoue
M
M
McGregor
S E
SE
Nation
J G
JG
D015331:Cohort Studies; D003127:Colposcopy; D003218:Condylomata Acuminata / Q000175:diagnosis*; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D015151:Immunoblotting; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics*; D014412:Tumor Virus Infections / Q000175:diagnosis / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000473:pathology; D014626:Vaginal Smears / Q000295:instrumentation*
D004279:DNA, Viral