title: Mn2(+)-binding properties of a recombinant protein-tyrosine kinase derived from the human insulin receptor.
abstract: The divalent cation-binding properties of the human insulin receptor tyrosine kinase domain were examined kinetically and by electron paramagnetic resonance and circular dichroic spectroscopy. The protein-tyrosine kinase activity of the purified cytoplasmic domain can be activated nearly 10-fold by 3 mM Mn2+ in the presence or absence of 5 mM Mg2+. Electron paramagnetic resonance spectra of the purified, acid-denatured kinase domain and assays of EDTA-treated kinase show that the purified protein does not possess residual, tightly bound Mn2+. Electron paramagnetic resonance spectroscopy was used to directly measure the binding constant of the kinase domain for Mn2+. The results indicate that the recombinant cytoplasmic domain of the human insulin receptor does not bind Mn2+ tightly in the absence or presence of MgATP (Kd greater than 0.8 mM). Furthermore, the enzyme does not show a strong preference for MnATP binding when both MgATP and MnATP are present. The far-ultraviolet circular dichroic spectrum of this domain is characterized by a negative maximum at 207 nm. In the presence of Mn2+, but not Mg2+, changes in the mean residue-weight ellipticity at 207 nm occur that are consistent with a decrease in alpha-helical content. The addition of ATP to Mn2(+)-bound protein does not further perturb the spectrum. We conclude that Mn2+ ions, although they bind weakly, induce an activating conformational change in the secondary structure of the human insulin receptor cytoplasmic domain. Activation by Mn2+ is unlikely to be significant in intact cells, but it may mimic the action of a physiological activator.
authors.affiliation: Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
authors.forename: S R
authors.initials: SR
authors.lastname: Wente
authors.forename: M
authors.initials: M
authors.lastname: Villalba
authors.forename: V L
authors.initials: VL
authors.lastname: Schramm
authors.forename: O M
authors.initials: OM
authors.lastname: Rosen
mesh_terms: D002942:Circular Dichroism; D004578:Electron Spin Resonance Spectroscopy; D006657:Histones / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008345:Manganese / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011972:Receptor, Insulin / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet
chemical_list: D006657:Histones; D011994:Recombinant Proteins; D008345:Manganese; D011505:Protein-Tyrosine Kinases; D011972:Receptor, Insulin