Epstein-Barr virus internalization and infectivity are blocked by selective protein kinase C inhibitors.
Selective protein kinase C inhibitors can either block or significantly reduce Epstein-Barr virus infectivity: inhibition of transformation and decreased 3H-thymidine (3H-TdR) incorporation in human B lymphocytes infected with B95-8 EBV, as well as a significant reduction in the induction of early antigens in Raji cells superinfected by P3HRI EBV was achieved by pre-treating the cells with the inhibitors. The inhibitors do not act by blocking binding of the virus to its cellular receptor CR 2, but rather are effective in the viral internalization process. Our results suggest that protein kinase C may be involved in the process of viral entry into cells.
Cirone
M
M
Dipartimento di Medicina Sperimentale, Università La Sapienza, Rome, italy.
Angeloni
A
A
Barile
G
G
Zompetta
C
C
Venanzoni
M
M
Torrisi
M R
MR
Frati
L
L
Faggioni
A
A
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000470:Alkaloids / Q000494:pharmacology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis / Q000378:metabolism; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002472:Cell Transformation, Viral / Q000187:drug effects; D004854:Herpesvirus 4, Human; D006801:Humans; D007244:Infectious Mononucleosis / Q000517:prevention & control*; D007546:Isoquinolines / Q000494:pharmacology*; D010879:Piperazines / Q000494:pharmacology*; D011951:Receptors, Complement / Q000032:analysis / Q000378:metabolism; D017464:Receptors, Complement 3d; D019311:Staurosporine
D000470:Alkaloids; D000944:Antigens, Differentiation, B-Lymphocyte; D000956:Antigens, Viral; D007546:Isoquinolines; D010879:Piperazines; D011951:Receptors, Complement; D017464:Receptors, Complement 3d; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D019311:Staurosporine