Early enhancement of calcium currents by H-ras oncoproteins injected into Hermissenda neurons.
Influx of calcium through membrane channels is an important initial step in signal transduction of growth signals. Therefore, the effects of Ras protein injection on calcium currents across the soma membrane of an identified neuron of the snail Hermissenda were examined. With the use of these post-mitotic cells, a voltage-sensitive, inward calcium current was increased 10 to 20 minutes after Harvey-ras oncoproteins were injected. The effects of oncogenic Harvey ras p21 protein (v-Ras) occurred quickly and were sustained, whereas the effects of proto-oncogenic ras protein (c-Ras) were transient. This relative potency is consistent with the activities of these oncoproteins in stimulating cell proliferation. Thus, this calcium channel may be a target for Ras action.
Collin
C
C
Laboratory of Molecular and Cellular Neurobiology, NINDS-NIH, Bethesda, MD 20892.
Papageorge
A G
AG
Lowy
D R
DR
Alkon
D L
DL
D000818:Animals; D001464:Barium / Q000494:pharmacology; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D008845:Microinjections; D009474:Neurons / Q000502:physiology*; D015689:Oncogene Protein p21(ras) / Q000008:administration & dosage / Q000494:pharmacology*; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology; D016283:Proto-Oncogene Proteins p21(ras) / Q000008:administration & dosage / Q000494:pharmacology*; D012908:Snails
D015220:Calcium Channels; D015221:Potassium Channels; D001464:Barium; D015689:Oncogene Protein p21(ras); D016283:Proto-Oncogene Proteins p21(ras)