Different effects of cinnamic acid on the O2- generation by guinea pig macrophages stimulated with a chemotactic peptide and immune complex.
Cinnamic acid inhibits the O2(-)-generating response of guinea pig peritoneal macrophages elicited with a chemotactic peptide, N-formyl-methionyl-leucyl-phenylalanine (fMLP), but not those with ovalbumin complex of immunoglobulin G2 antibody and phorbol-myristate acetate. During the course of study on the inhibitory mechanism of cinnamic acid, we found that the acid also inhibited the Ca2+ mobilization elicited with fMLP, but not that with the immune complex. In addition, the treatment of macrophages with Ionomycin and ethyleneglycol bis-(beta-aminoethylether)-N,N'-tetraacetic acid for depletion of the intracellular Ca2+ inactivated completely the O2- generation elicited with fMLP, but not its counterpart of the immune complex. Thus, the inhibitory activity of cinnamic acid on the O2- generation elicited with fMLP seems partly due to that on the Ca2+ mobilization. On the other hand, cinnamic acid augmented the intracellular accumulation of adenosine 3',5'-cyclic monophosphate (cyclic AMP) in the presence of 3-isobutyl 1-methylxanthine (IBMX), and elevated more intensively the concentration of cyclic AMP when macrophages were stimulated with fMLP. Since IBMX inhibited the O2- generation elicited with fMLP, the enhancement of activation of an adenylate cyclase by cinnamic acid might cause depression of the O2- generation. This possibility, however, seems to be excluded by the fact that the same effect of cinnamic acid was observed even when macrophages were stimulated with the immune complex.
Imamichi
T
T
Faculty of Pharmaceutical Sciences, Hokkaido University, Japan.
Nakamura
T
T
Hayashi
K
K
Kaneko
K
K
Koyama
J
J
D000818:Animals; D000936:Antigen-Antibody Complex / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002630:Chemotactic Factors / Q000494:pharmacology; D002934:Cinnamates / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism; D006168:Guinea Pigs; D007424:Intracellular Fluid / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000378:metabolism / Q000494:pharmacology*; D011971:Receptors, Immunologic / Q000494:pharmacology; D013268:Stimulation, Chemical; D013481:Superoxides / Q000378:metabolism*
D000936:Antigen-Antibody Complex; D002630:Chemotactic Factors; D002934:Cinnamates; D011971:Receptors, Immunologic; D013481:Superoxides; C029010:cinnamic acid; D009240:N-Formylmethionine Leucyl-Phenylalanine; D000242:Cyclic AMP; D002118:Calcium