[M2-type pyruvate kinase in the diagnosis of hepatocarcinoma--a pilot study].
By using Fab'-enzyme labelled immunosorbent assay (ELISA) at a sensitivity of picogram (10(-12) g or pg) level, M-type pyruvate kinase (M-PyK) in the plasma was determined in 47 healthy adults and 26 hepatocellular carcinoma (HCC) patients. The upper limits of M-PyK in the normal male and female were 1.1 and 1.4 ng/ml. The plasma M-PyK in HCC patients was significantly increased about 5 fold of the average normal level with a positive rate of about 95%. In 6 cases of subclinical small hepatocarcinoma and 7 cases of HCC with normal serum alpha-fetoprotein level, the plasma M-PyK was increased, whereas the plasma M-PyK levels were normal in acute or chronic hepatitis and the other benign diseases. After tumor resection, the plasma M-PyK returned to the normal value, but increased again in cases with recurrence, suggesting that the increased M-PyK in the plasma of HCC patients originates from M2-type PyK in HCC tissue. The plasma M2-PyK also increased in patients with carcinoma of the digestive tract, so M2-PyK may become a new marker of malignant tumors.
Liu
J B
JB
Shanghai Medical College.
D014408:Biomarkers, Tumor / Q000097:blood*; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis*; D004796:Clinical Enzyme Tests; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D007527:Isoenzymes / Q000097:blood*; D008113:Liver Neoplasms / Q000175:diagnosis*; D008297:Male; D009364:Neoplasm Recurrence, Local; D010865:Pilot Projects; D011770:Pyruvate Kinase / Q000097:blood*
D014408:Biomarkers, Tumor; D007140:Immunoglobulin Fab Fragments; D007527:Isoenzymes; D011770:Pyruvate Kinase