title: Direct demonstration of guanine nucleotide sensitive receptors for vasoactive intestinal peptide in the anterior lobe of the rat pituitary gland.
abstract: The vasoactive intestinal peptide (VIP) receptors were identified on the membranes from the rat anterior pituitary gland with [125I]VIP. The dissociation constant (Kd) and the maximal binding capacity (Bmax) values were estimated from the competitive inhibition data. The Kd and Bmax values were 1.05 +/- 0.75 nM and 103 +/- 11 fmol/mg protein, respectively. The order of molar potency of related peptides to inhibit [125I]VIP binding was VIP greater than peptide histidine isoleucine (PHI) greater than secretin greater than glucagon. Glucagon was not effective to inhibit the binding. [125I]VIP binding was effectively inhibited by the addition of guanine nucleotides. The order of molar potency to inhibit the binding was Gpp(NH)p greater than GTP greater than GDP greater than GMP greater than ATP. These results directly suggest the coupling of VIP receptors with guanine nucleotide binding proteins in the anterior pituitary gland.
authors.affiliation: Institute for Animal Experimentation, University of Tokushima School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Agui
authors.forename: K
authors.initials: K
authors.lastname: Matsumoto
mesh_terms: D000818:Animals; D001667:Binding, Competitive / Q000502:physiology; D006150:Guanine Nucleotides / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008297:Male; D010903:Pituitary Gland, Anterior / Q000032:analysis*; D011869:Radioligand Assay; D051381:Rats; D011919:Rats, Inbred Strains; D011964:Receptors, Gastrointestinal Hormone / Q000032:analysis*; D018005:Receptors, Vasoactive Intestinal Peptide
chemical_list: D006150:Guanine Nucleotides; D007457:Iodine Radioisotopes; D011964:Receptors, Gastrointestinal Hormone; D018005:Receptors, Vasoactive Intestinal Peptide