Chromate reduction in Streptomyces.
Streptomyces species 3M grew in peptone yeast extract medium with 1000 micrograms/ml K2Cr2O7. Incubation of the chromate with different cell fractions in the presence of NADH and NADPH resulted in a decrease of Cr6+ in the reaction mixture. The level of Cr6+ was reduced by 82.7% by a particulate cell fraction obtained by centrifugation at 105,000 x g for 1 h, in the presence of NADH. The reducing enzyme was associated with this cell fraction. The enzyme was constitutive and reduced Cr6+ to Cr3+.
Das
S
S
Department of Microbiology, Bose Institute, Calcutta, India.
Chandra
A L
AL
D002840:Chromates / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D010084:Oxidation-Reduction; D013302:Streptomyces / Q000201:enzymology / Q000378:metabolism*
D002840:Chromates