Insertion of IS4 into the tnpA gene of Tn3: sequencing of the insertion site.
Plasmid pMM237 lacks the deletion-forming activity associated with its Tn3 transposase-encoding gene (mpA) [Morita et al., J. Biochem. 101 (1987) 1253-1264]. Analysis of the plasmid DNA showed that pMM237 was generated by insertion of IS4 into the tnpA gene of the original plasmid pMM234. The insertion was accompanied by a direct 12-bp duplication at the target site. The nucleotide sequence around the target site shared only some of the characteristics of previously reported IS4 target sites [Habermann et al., Mol. Gen. Genet. 175 (1979) 369-373; Klaer et al., Mol. Gen. Genet. 181 (1981) 169-175; Georgopoulos et al., Gene 20 (1982) 83-90; Mayaux et al., Gene 30 (1984) 137-146].
Morita
M
M
Laboratory of Biochemistry, Kansai Medical University, Osaka, Japan.
Sugino
Y
Y
D001345:Autoradiography; D001483:Base Sequence; D003062:Codon; D004251:DNA Transposable Elements; D004274:DNA, Recombinant / Q000235:genetics*; D015870:Gene Expression; D008969:Molecular Sequence Data; D009713:Nucleotidyltransferases / Q000235:genetics*; D010957:Plasmids; D019895:Transposases
D003062:Codon; D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D009713:Nucleotidyltransferases; D019895:Transposases