Glucose-stimulated phosphorylation of the 64-kDa protein of human polymorphonuclear leukocytes in a cell-free system.
Glucose-stimulated phosphorylation of 64-kDa protein using a greater than 30 kDa fraction of human polymorphonuclear leukocytes in a dose-dependent fashion with 33 microM for maximum stimulation and 1.4 microM for ED50. None of the glucose derivatives and metabolites of glycolysis stimulated phosphorylation, but glucose-1-phosphate, glucose-6-phosphate, fructose-6-phosphate and fructose-1,6-diphosphate inhibited the glucose-stimulated phosphorylation, strongly suggesting phosphorylation by glucose-regulated protein kinase. Cyclic nucleotides and the protein kinase inhibitors H-7, H-8, W-7 and staurosporine did not affect phosphorylation.
Shibata
M
M
Department of Antibiotics, National Institute of Health, Tokyo, Japan.
Koshio
O
O
Ohoka
T
T
Mizuno
S
S
Suzuki
K
K
D002474:Cell-Free System; D000242:Cyclic AMP / Q000494:pharmacology; D006152:Cyclic GMP / Q000494:pharmacology; D005947:Glucose / Q000031:analogs & derivatives / Q000494:pharmacology*; D006019:Glycolysis; D006801:Humans; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009363:Neoplasm Proteins / Q000494:pharmacology; D009504:Neutrophils / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology; D010766:Phosphorylation; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000187:drug effects*; D011506:Proteins / Q000378:metabolism*; D013268:Stimulation, Chemical
D009363:Neoplasm Proteins; D010446:Peptide Fragments; D047428:Protein Kinase Inhibitors; D011506:Proteins; D009240:N-Formylmethionine Leucyl-Phenylalanine; D000242:Cyclic AMP; D011494:Protein Kinases; D006152:Cyclic GMP; D005947:Glucose