title: Thrombus detection using a Tc-99m labeled antifibrin monoclonal antibody (MoAb). Experiments in vitro and in animals.
abstract: This paper describes the production and characterization of an antifibrin monoclonal antibody, produced by immunizing mice with fibrinogen degradation fragment Y. The antibody (designated Y22) is directed against a conformation dependent epitope in the D-domain of fibrin Y22 was labeled with the radionuclide Tc-99m. Experiments in vitro and in rats are presented which show the potential of scintigraphic detection of thrombi with Tc-99m-Y22. Preliminary results show that immunoscintigraphy of thrombi may also have potential for the monitoring of thrombolytic therapy (eg with t-PA).
authors.affiliation: Gaubius Institute TNO, Leiden, The Netherlands.
authors.forename: M N
authors.initials: MN
authors.lastname: Wasser
authors.forename: E K
authors.initials: EK
authors.lastname: Pauwels
authors.forename: W
authors.initials: W
authors.lastname: Nieuwenhuizen
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D003429:Cross Reactions / Q000276:immunology; D005337:Fibrin / Q000276:immunology*; D005342:Fibrinolysis; D006801:Humans; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D011877:Radionuclide Imaging; D051381:Rats; D011919:Rats, Inbred Strains; D013667:Technetium; D013927:Thrombosis / Q000000981:diagnostic imaging*; D014682:Vena Cava, Inferior / Q000000981:diagnostic imaging
chemical_list: D000911:Antibodies, Monoclonal; D013667:Technetium; D005337:Fibrin