Demonstration of lactogenic receptors in rat endocrine pancreases by quantitative autoradiography.
A direct effect of growth hormone and/or prolactin on the growth of the pancreatic beta-cell has been proposed. This study assessed the presence of human growth hormone (hGH)-binding sites in male adult rat endocrine pancreas via quantitative autoradiography. The binding of 125I-labeled hGH was evaluated by receptor autoradiography on frozen-pancreas cryostat cut sections. The sections were incubated with 125I-hGH (10(-10) M) for 75 min at room temperature, and nonspecific binding was determined in the presence of excess native hGH (5 X 10(-7) M). The specificity of the binding was assessed in competition experiments with bovine GH and ovine prolactin. The autoradiograms were quantified with a computer-assisted image-processing system. The sections were then stained to visualize the endocrine islets. Nondiabetic control and streptozocin (STZ)-injected rats were used. Our results show that 1) there is specific binding of iodinated hGH in small areas of the pancreas, which appear as the Langerhans islets when the autoradiogram and the stained sections are superimposed; 2) the specificity of hGH binding in rat islets is lactogenic; 3) the density of the hGH-binding sites in the endocrine pancreas is estimated at 4.8 fmol/mg protein, with IC50 ranging from 0.98 to 2.50 nM; and 4) binding sites may be present on the beta-cell, because specific binding disappears in STZ-injected rats. In conclusion, by use of a quantitative autoradiographic technique, we provide evidence for the presence of lactogenic receptors on rat beta-cells.
Polak
M
M
National Institute of Health and Medical Research, Unit 30, Paris, France.
Scharfmann
R
R
Ban
E
E
Haour
F
F
Postel-Vinay
M C
MC
Czernichow
P
P
D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D013006:Growth Hormone / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D007457:Iodine Radioisotopes; D007515:Islets of Langerhans / Q000378:metabolism*; D008297:Male; D011388:Prolactin / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000378:metabolism*
D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface; D011388:Prolactin; D013006:Growth Hormone