title: Does 3,5,dibromo-4-nitrosobenzene sulphonate spin trap superoxide radicals?
abstract: Pulse radiolysis studies show that the spin trap 3,5,dibromo-4-nitrosobenzene sulphonate (I) reacts rapidly with O2.- but the product formed is very unstable. No radicals were detected in ESR studies of solutions of I after reaction with O2.- formed by gamma-radiolysis. Evidence is presented that the stable radical observed by some, but not all workers, following exposure of I to the O2.(-)-generating xanthine/xanthine oxidase system, is produced by a peroxidatic oxidation using hydrogen peroxide formed by O2-. dismutation and that formation of this radical depends on the presence of peroxidase activity in the xanthine oxidase sample employed.
authors.affiliation: Department of Chemistry, University of Newcastle upon Tyne, U.K.
authors.forename: N B
authors.initials: NB
authors.lastname: Nazhat
authors.forename: G
authors.initials: G
authors.lastname: Yang
authors.forename: R E
authors.initials: RE
authors.lastname: Allen
authors.forename: D R
authors.initials: DR
authors.lastname: Blake
authors.forename: P
authors.initials: P
authors.lastname: Jones
mesh_terms: D001557:Benzenesulfonates; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D009603:Nitroso Compounds; D013481:Superoxides; D014969:Xanthine Oxidase
chemical_list: D001557:Benzenesulfonates; D005609:Free Radicals; D009603:Nitroso Compounds; D013481:Superoxides; C048757:3,5-dibromo-4-nitrosobenzenesulfonate; D014969:Xanthine Oxidase