The induction of aneuploidy by 3-nitrobenzo[a]pyrene in Chinese hamster ovary cells.
Nitrobenzo[a]pyrenes are found in urban air particulates and particulates from diesel exhaust, gasoline engines and wood burning stoves. Following exposure of Chinese hamster ovary cells (CHO-K1-BH4) to 3-nitrobenzo[a]pyrene (3-NB[a]P), cells with multiple nuclei and/or nuclei with multiple lobes were observed. When CHO cells were treated with 5 micrograms/ml 3-NB[a]P for 5 or 20 h, aneuploidy was noted in these cells at 24-96 h post-exposure. The addition of N6, O2'-dibutyryl adenosine 3':5'-cyclic monophosphate to 3-NB[a]P-exposed CHO cell cultures appeared to reduce the amount of aneuploidy in treated cultures. Structure--activity studies showed that 1-NB[a]P was a much less effective inducer of aneuploidy than 3-NB[a]P and 6-NB[a]P was ineffective. 1-, 3- and 6-nitrosobenzo[a]pyrenes were not effective inducers of aneuploidy in CHO cells, and aneuploidy was not observed in cultures treated with 3-NB[a]P in the presence of S9 activation. It appears that the parent 3-NB[a]P is responsible for producing aneuploidy in CHO cells.
Neft
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Department of Health and Human Services, Food and Drug Administration, Jefferson, AR 72079.
Schol
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Fu
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Casciano
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DA
D000782:Aneuploidy; D000818:Animals; D001580:Benzopyrenes / Q000494:pharmacology*; D001711:Biotransformation; D002875:Chromosomes / Q000187:drug effects / Q000648:ultrastructure*; D006224:Cricetinae; D003412:Cricetulus; D000242:Cyclic AMP / Q000494:pharmacology; D005260:Female; D005787:Gene Frequency; D066298:In Vitro Techniques; D009153:Mutagens; D010053:Ovary / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D001580:Benzopyrenes; D009153:Mutagens; C042055:3-nitrobenzo(a)pyrene; D000242:Cyclic AMP