[The chromatography of yeast recombinant HBsAg in porous silicas].
The results of the study of adsorption processes of recombinant HBsAg on various porous silicas are presented. The maximal yield of the recombinant antigen by absorption chromatography was observed on unmodified macroporous glass MPS-2000. A mean yield in this case was 85%. Silica C-80 modified with polyvinylpyrrolidone proved to be most acceptable for gel-penetrating chromatography. The adsorption and gel-penetrating chromatography on porous silicas proved to be an effective method for purification of recombinant HBsAg.
Kurbatova
I Iu
IIu
Krasil'nikov
I V
IV
Borisova
V N
VN
Zubov
S V
SV
D000327:Adsorption; D002845:Chromatography / Q000379:methods*; D002850:Chromatography, Gel; D005656:Fungal Proteins / Q000032:analysis / Q000302:isolation & purification*; D006514:Hepatitis B Surface Antigens / Q000032:analysis / Q000302:isolation & purification*; D016062:Porosity; D011994:Recombinant Proteins / Q000032:analysis / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae; D012822:Silicon Dioxide
D005656:Fungal Proteins; D006514:Hepatitis B Surface Antigens; D011994:Recombinant Proteins; D012822:Silicon Dioxide
Khromatografiia drozhzhevogo rekombinantnogo HBsAg na poristykh kremnezemakh.