The effect of plasmid pKM101 on umuDC gene function enhancing precise excision of transposons.
Mutation in the thr gene with the highest UV-induced reversion frequency has been chosen from a number of mutations arisen from insertion of transposon Tn10 into the umuDC- Salmonella typhimurium. This mutation has been transferred by transduction into the Salmonella strain carrying E. coli umuDC+ genes. umuDC- and umuDC+ Salmonella strains, bearing the same insertion mutation, were used to study the effect of umuDC genes and of plasmid pKM101 on spontaneous and UV-induced precise excision of Tn10. This process, like point mutagenesis, is shown to be umuDC-stimulated. Plasmid pKM101 eliminates the effect of umuDC+ genes on UV-induced Tn10 precise excision, in contrast to its effect on point mutagenesis, and does not enhance spontaneous precise excision of Tn10.
Andreeva
I V
IV
Gamaleya Institute for Epidemiology and Microbiology, Academy of Medical Sciences of the U.S.S.R., Moscow.
Rusina OYu
Mirskaya
E E
EE
Skavronskaya
A G
AG
D002876:Chromosomes, Bacterial; D004251:DNA Transposable Elements / Q000528:radiation effects; D005798:Genes, Bacterial; D010957:Plasmids; D012486:Salmonella typhimurium / Q000235:genetics*; D014161:Transduction, Genetic; D014466:Ultraviolet Rays
D004251:DNA Transposable Elements