An ELISA for detecting pestivirus antigen in the blood of sheep persistently infected with border disease virus.
A monoclonal antibody capture enzyme-linked immunosorbent assay (ELISA) has been developed to detect a pestivirus-specific antigen in leucocytes of sheep persistently infected with border disease virus. A blind trial was conducted to compare the specificity of the ELISA with conventional tissue culture virus isolation on blood samples from 58 sheep, aged 3 to 48 months. There was total agreement between the two tests; 27 sheep were shown to be BDV-infected. The ELISA OD values of the positive samples ranged from 0.12 to 0.86 and were not related to age, strain of virus with which they were infected or presence of serum neutralising antibody. Negative samples had OD values between 0 and 0.02.
Fenton
A
A
Moredun Research Institute, Edinburgh, Scotland, U.K.
Entrican
G
G
Herring
J A
JA
Nettleton
P F
PF
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000097:blood*; D001882:Border Disease / Q000175:diagnosis* / Q000382:microbiology; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D010576:Pestivirus / Q000276:immunology* / Q000302:isolation & purification; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis*
D000914:Antibodies, Viral; D000956:Antigens, Viral