title: In vitro comparison of HMPAO and gentisic acid for labelling leukocytes with 99mTc.
abstract: Leukocytes can be labelled with 99mTc using HMPAO and gentisic acid methods. We compared the two methods with respect to labelling efficiency on mixed leukocytes and isolated polymorphonuclear (PMN) and mononuclear (MN) cells, and the in vitro stability of the label. HMPAO produced approximately 70% labelling efficiency on mixed or PMN cells and the label was stable in saline or plasma. Labelling efficiency on MN was only 14% and was less stable. Gentisic acid produced a labelling efficiency of 52% on PMN and 35% on MN; both were stable in saline but less stable in plasma. In conclusion, HMPAO produces higher labelling efficiency and the label shows greater in vitro stability in plasma. However, gentisic acid is much less expensive to use, allows labelling of MN cells, and should result in more favourable microdosimetry. Preliminary clinical results suggest that gentisic acid is equivalent to HMPAO but has the advantage of being much cheaper.
authors.affiliation: Division of Nuclear Medicine, Ottawa Civic Hospital, Ontario, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Ecclestone
authors.forename: A
authors.initials: A
authors.lastname: Proulx
authors.forename: J R
authors.initials: JR
authors.lastname: Ballinger
authors.forename: B
authors.initials: B
authors.lastname: Gerson
authors.forename: R H
authors.initials: RH
authors.lastname: Reid
authors.forename: K Y
authors.initials: KY
authors.lastname: Gulenchyn
mesh_terms: D003365:Costs and Cost Analysis; D005841:Gentisates; D006801:Humans; D062385:Hydroxybenzoates; D066298:In Vitro Techniques; D007553:Isotope Labeling / Q000379:methods; D007962:Leukocytes; D007963:Leukocytes, Mononuclear; D009504:Neutrophils; D015609:Organotechnetium Compounds; D010091:Oximes; D013670:Sodium Pertechnetate Tc 99m; D019690:Technetium Tc 99m Exametazime
chemical_list: D005841:Gentisates; D062385:Hydroxybenzoates; D015609:Organotechnetium Compounds; D010091:Oximes; D019690:Technetium Tc 99m Exametazime; D013670:Sodium Pertechnetate Tc 99m; C010925:2,5-dihydroxybenzoic acid