Trans-complementation of the C gene of human and the P gene of woodchuck hepadnaviruses.
A 5 bp insertion was introduced into the BstEII site at nucleotide 2815 in DNA of hepatitis B virus (HBV) and a mutant HBV genome was produced, which coded for envelope and core proteins, but not for DNA polymerase, due to a frameshift. Cultured hepatoma cells (HepG2) were simultaneously transfected with a plasmid harbouring a tandem dimer of the mutant HBV DNA and another plasmid harbouring a tandem dimer of DNA of woodchuck hepatitis virus or duck hepatitis B virus. The replication of mutant HBV DNA, incapable of encoding DNA polymerase, was accomplished by cotransfecting woodchuck hepatitis virus DNA, but not by duck hepatitis B virus DNA. These results indicated a trans-complementation of the C and P genes in mammalian hepadnaviruses beyond a species barrier.
Okamoto
H
H
Immunology Division, Jichi Medical School, Tochigi-Ken, Japan.
Omi
S
S
Wang
Y
Y
Imai
M
M
Mayumi
M
M
D000818:Animals; D015139:Blotting, Southern; D006528:Carcinoma, Hepatocellular; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D015613:Hepadnaviridae / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms; D008392:Marmota; D009693:Nucleic Acid Hybridization; D014162:Transfection; D014407:Tumor Cells, Cultured; D014758:Viral Core Proteins / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics; D014779:Virus Replication
D004279:DNA, Viral; D014758:Viral Core Proteins; D014759:Viral Envelope Proteins; D004259:DNA-Directed DNA Polymerase