Effect of tissue specificity of brain soluble fractions on Na+, K(+)-ATPase activity.
Previous evidence from this laboratory indicated that catecholamines and brain endogenous factors modulate Na+,K(+)-ATPase activity of the synaptosomal membranes. The filtration of a brain total soluble fraction through Sephadex G-50 permitted the separation of two fractions -peaks I and II-which stimulated and inhibited Na+,K(+)-ATPase, respectively (Rodríguez de Lores Arnaiz and Antonelli de Gomez de Lima, Neurochem. Res. 11, 1986, 933). In order to study tissue specificity a rat kidney total soluble was fractionated in Sephadex G-50 and kidney peak I and II fractions were separated; as control, a total soluble fraction prepared from rat cerebral cortex was also processed. The UV absorbance profile of the kidney total soluble showed two zones and was similar to the profile of the brain total soluble. Synaptosomal membranes Na+,K(+)- and Mg2(+)-ATPases were stimulated 60-100% in the presence of kidney and cerebral cortex peak I; Na+,K(+)-ATPase was inhibited 35-65% by kidney peak II and 60-80% by brain peak II. Mg2(+)-ATPase activity was not modified by peak II fractions. ATPases activity of a kidney crude microsomal fraction was not modified by kidney peak I or brain peak II, and was slightly increased by kidney peak II or brain peak I. Kidney purified Na+,K(+)-ATPase was increased 16-20% by brain peak I and II fractions. These findings indicate that modulatory factors of ATPase activity are not exclusive to the brain. On the contrary, there might be tissue specificity with respect to the enzyme source.
Rodríguez de Lores Arnaiz
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Instituto de Biologia Celular, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D017301:Ca(2+) Mg(2+)-ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002540:Cerebral Cortex / Q000201:enzymology; D002850:Chromatography, Gel; D005260:Female; D007668:Kidney / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D008861:Microsomes / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013574:Synaptosomes / Q000201:enzymology
D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase