title: Detection of activated c-H-ras oncogene in hepatocellular carcinomas developing in transgenic mice harboring albumin promoter-regulated simian virus 40 gene.
abstract: Hepatocellular carcinomas (HCCs) developing in transgenic mice harboring an albumin promoter-regulated simian virus 40 (SV40) tumor antigen gene were analyzed for activating point mutations of the c-H-ras oncogene. Oligonucleotide hybridization studies utilizing enzymatically amplified DNA sequences of paraffin-embedded tumor tissues revealed that 10 out of 25 HCCs contained either an A-to-T or an A-to-G conversion at the second position of codon 61. Northern blot studies confirmed expression of SV40 gene mRNAs in the tumor tissues of both mutation-positive and mutation-negative HCCs. These findings in association with earlier results thus strongly suggest that mutational activation of cellular oncogene may play an important role in SV40-initiated multistage hepatocarcinogenesis in vivo.
authors.affiliation: Department of Pathology, Cancer Institute, Tokyo, Japan.
authors.forename: G H
authors.initials: GH
authors.lastname: Lee
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: K
authors.initials: K
authors.lastname: Ohtake
authors.forename: K
authors.initials: K
authors.lastname: Nomura
authors.forename: O
authors.initials: O
authors.lastname: Hino
authors.forename: Y
authors.initials: Y
authors.lastname: Furuta
authors.forename: S
authors.initials: S
authors.lastname: Aizawa
authors.forename: T
authors.initials: T
authors.lastname: Kitagawa
mesh_terms: D000418:Albumins / Q000235:genetics*; D000818:Animals; D000957:Antigens, Viral, Tumor / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D011905:Genes, ras; D008114:Liver Neoplasms, Experimental / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection
chemical_list: D000418:Albumins; D000957:Antigens, Viral, Tumor; D012333:RNA, Messenger; D012367:RNA, Viral