Role of nucleotide pyrophosphatase in enflurane-induced reduction of UDP-glucuronic acid concentration in mouse liver.
Because hepatic UDP-glucuronic acid levels decrease upon exposure to volatile anesthetics, the present study was designed to determine the mechanism by which enflurane decreases UDP-glucuronic acid in mice by measuring the concentrations of intermediates and the activities of enzymes in the UDP-glucuronic acid pathway. UDP-glucuronic acid concentrations were decreased by 40% in both male and female mice after 10 min of enflurane-induced narcosis. Concentration of UDP-glucose and the activities of diethylstilbestrol UDP-glucuronosyltransferase and UDP-glucose dehydrogenase were not affected by enflurane treatment. In contrast, nucleotide pyrophophatase activity was increased approximately 50% in both sexes. Thus, the decrease in hepatic UDP-glucuronic acid upon exposure of mice to enflurane is probably due to increased degradation by nucleotide pyrophosphatase.
Watkins
J B
JB
Medical Science Program, Indiana University School of Medicine, Bloomington 47405.
Pierce
M A
MA
D000818:Animals; D004737:Enflurane / Q000494:pharmacology*; D005260:Female; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology*; D011755:Pyrophosphatases / Q000378:metabolism*; D014535:Uridine Diphosphate Glucuronic Acid / Q000378:metabolism*; D014539:Uridine Diphosphate Sugars / Q000378:metabolism*
D014539:Uridine Diphosphate Sugars; D014535:Uridine Diphosphate Glucuronic Acid; D004737:Enflurane; D011755:Pyrophosphatases; C021899:nucleotide pyrophosphatase