title: Dehydration increases the density of C receptors for ANF on rat glomerular membranes.
abstract: The present study determined the presence of two types of binding sites for atrial natriuretic factor (ANF), the B and C receptor, on rat glomerular membranes. The effect of short-term salt loading and dehydration on these two receptor populations was investigated consecutively. Salt-loaded rats did not show significant changes in plasma ANF concentrations or in the number of ANF binding sites. Water-deprived rats presented significantly lower plasma ANF concentrations (22.0 +/- 1.9 vs. 34.4 +/- 3.8 fmol/ml, P less than 0.01) and an increase in total receptor density (1,860 +/- 398 vs. 987 +/- 143 fmol/mg protein) as compared with the control group. Differentiation of both receptor populations showed that it was the C receptors that accounted for this increase (1,772 +/- 369 vs. 901 +/- 151 fmol/mg protein, P less than 0.05), whereas B-receptor density was unchanged (89 +/- 31 vs. 87 +/- 44 fmol/mg protein). These data suggest that C receptors for ANF are affected by changes of body fluid volume.
authors.affiliation: Department of Medicine II, Klinikum Grosshadern, Munich, Federal Republic of Germany.
authors.forename: M C
authors.initials: MC
authors.lastname: Kollenda
authors.forename: A M
authors.initials: AM
authors.lastname: Vollmar
authors.forename: G A
authors.initials: GA
authors.lastname: McEnroe
authors.forename: A L
authors.initials: AL
authors.lastname: Gerbes
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism; D001665:Binding Sites; D003681:Dehydration / Q000378:metabolism*; D007678:Kidney Glomerulus / Q000378:metabolism*; D008297:Male; D008566:Membranes / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D013997:Time Factors; D014868:Water Deprivation / Q000502:physiology
chemical_list: D011956:Receptors, Cell Surface; D012965:Sodium Chloride; D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor