title: Superoxide radical as electron donor for oxidative phosphorylation of ADP.
abstract: When isolated rat heart mitochondria are subject to xanthine/xanthine oxidase generated free radicals, nmol quantities of ADP are phosphorylated to ATP. This effect is proportional to xanthine oxidase concentration, and is relatively independent of ADP concentration. Exogenous superoxide dismutase partially suppresses the phosphorylation. Micromolar concentrations of iron salts completely eliminate the phosphorylation. Catalase has no effect. The likely electron source, then, is superoxide radicals. The reduced minus oxidised spectra of superoxide-bombarded mitochondria show that superoxide enters the electron transport chain by reducing cytochrome c and complex IV. Mitochondria retain their ability to phosphorylate ADP in more traditional ways under the experimental conditions described. Superoxide under physiological conditions in vivo may be a source of electrons for the oxidative phosphorylation of ADP.
authors.affiliation: Saint Mary's University, Halifax, N.S., Canada.
authors.forename: K
authors.initials: K
authors.lastname: Mailer
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism*; D000818:Animals; D004579:Electron Transport; D005609:Free Radicals; D066298:In Vitro Techniques; D008297:Male; D008929:Mitochondria, Heart / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D051381:Rats; D011919:Rats, Inbred Strains; D013481:Superoxides / Q000378:metabolism*; D019820:Xanthine; D014969:Xanthine Oxidase; D014970:Xanthines
chemical_list: D005609:Free Radicals; D014970:Xanthines; D013481:Superoxides; D019820:Xanthine; D000244:Adenosine Diphosphate; D014969:Xanthine Oxidase