A sensitive method of screening for dominant T cell clones by amplification of T cell gamma gene rearrangements with the polymerase chain reaction.
A sensitive method of screening for dominant T cell clones in small samples of DNA has been developed using the polymerase chain reaction to amplify and identify T cell gamma receptor gene rearrangements. It can detect such rearrangements in nanogram quantities of DNA from cultured T cell clones, even in the presence of 20-100 parts of polyclonal lymph node DNA, and works with DNA extracted from paraffin sections of cloned T cells which have been fixed in formalin. Presumptive clonal reactions have been obtained in preliminary tests on 8 of 10 unfixed T cell lymphomas but in 0 of 10 reactive lymph nodes.
Goudie
R B
RB
University Department of Pathology, Glasgow Royal Infirmary, U.K.
Karim
S N
SN
Mills
K
K
Alcorn
M
M
Lee
F D
FD
D001483:Base Sequence; D002460:Cell Line; D002999:Clone Cells / Q000166:cytology; D004247:DNA / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006801:Humans; D008223:Lymphoma / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D013601:T-Lymphocytes / Q000166:cytology*
D004247:DNA