Survey of fructose 1,6-bisphosphatase isoenzyme in rat organs and ontogenic expression of the enzyme in rat fetus.
1. Among eleven tissues of rat, the liver type of fructose 1,6-bisphosphatase (FBPase) subunit was detected in the liver, kidney, testis, pancreas and lung by Western blot analysis using anti-(liver FBPase) or anti-(muscle FBPase) serum. 2. The muscle type of the enzyme subunit was detected only in the pancreas other than skeletal muscle. Both types of the enzyme subunit were found in the pancreas. 3. Neither anti-(liver FBPase) nor anti-(muscle FBPase) serum detected the band of enzyme subunit on the blots of the extracts of brain, heart, small intestinal mucosa, spleen and placenta. 4. FBPase is present in fetal rat liver at least as early as the 14th day of gestation. 5. In agreement with the increase in immunological staining density, the level of the enzyme activity in fetal liver increased exponentially during fetal development. 6. The muscle enzyme was not detected until the fetus reached the 19th day of gestation.
Mizunuma
H
H
Department of Biochemistry, Akita University School of Medicine, Japan.
Tashima
Y
Y
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D015153:Blotting, Western; D005260:Female; D005333:Fetus / Q000201:enzymology*; D006597:Fructose-Bisphosphatase / Q000032:analysis*; D005865:Gestational Age; D007527:Isoenzymes / Q000032:analysis*; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000196:embryology / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008297:Male; D009132:Muscles / Q000196:embryology / Q000201:enzymology; D010179:Pancreas / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000201:enzymology; D014018:Tissue Distribution
D007527:Isoenzymes; D006597:Fructose-Bisphosphatase