Crystallization of recombinant rat cathepsin B.
A glycosylation-minus mutant of rat cathepsin B expressed in yeast has been purified and crystallized. X-ray diffraction data have been collected and molecular replacement for solving the structure is in progress. The space group for the recombinant rat cathepsin B was determined to be P2(1) with unit cell dimensions alpha = 62.2 A, b = 90.19 A, c = 47.07 A, and beta = 97.43 degrees. A unit cell contains 4 molecules and 2 molecules per asymmetric unit.
Lee
X
X
Division of Biological Sciences, National Research Council of Canada, Ottawa, Ontario.
Ahmed
F R
FR
Hirama
T
T
Huber
C P
CP
Rose
D R
DR
To
R
R
Hasnain
S
S
Tam
A
A
Mort
J S
JS
D000818:Animals; D002401:Cathepsin B / Q000235:genetics / Q000302:isolation & purification*; D003001:Cloning, Molecular; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D051381:Rats; D011994:Recombinant Proteins / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000235:genetics
D011994:Recombinant Proteins; D002401:Cathepsin B