[Use of leukocyte-filtered, cytomegalovirus-antibody negative and irradiated cellular blood products].
This paper presents both quality requirements and indications for use of leucocyte-filtered, cytomegalovirus antibody negative and irradiated cellular blood products at Rikshospitalet. Emphasis is placed on the use of standardized buffycoat depleted red cells or platelet concentrates for filtration, and the selection of leucocyte filters with high capacity and ease of bedside application. Leucocyte counts as low as 1-2 10(5) per unit are recommended after filtration in order to avoid HLA-antibody production. For bedside filtration, our choice was RC100 and PL100 (Pall) for red cells and platelets respectively. For laboratory use we prefer, for economic reasons, to use Sepacell R500 (Asahi) and Imugard IG500 (Terumo) for red cells and platelets respectively. Leucocyte-filtered blood products are considered indicated in all pre-transplant transfusions, in post-transplant HLA-sensitized patients, in other patients with febrile transfusion reactions, and in patients with an expected protracted platelet requirement. CMV antibody negative products are recommended for all immuno-deficient patients and pregnant women negative for CMV antibody. Irradiated blood products are used in the treatment of immuno-deficient patients receiving large amounts of blood, and in all severely immuno-compromised patients. In emergency situations where CMV antibody negative and/or irradiated blood products cannot be supplied, leucocyte filtration is suggested.
Solheim
B G
BG
Institutt for transplantasjonsimmunologi, Rikshospitalet, Oslo.
Albrechtsen
D H
DH
Evensen
S A
SA
Leivestad
T
T
D000914:Antibodies, Viral / Q000032:analysis; D001769:Blood / Q000528:radiation effects*; D001803:Blood Transfusion / Q000592:standards*; D003587:Cytomegalovirus / Q000276:immunology; D005374:Filtration; D006801:Humans; D007958:Leukocyte Count; D065227:Transfusion Reaction
D000914:Antibodies, Viral
Bruk av leukocyttfiltrerte, cytomegalovirus-antistoffnegative og bestrålte cellulaere blodprodukter.