Inhibitory effect of gossypol on human chorionic gonadotropin (hCG)-induced progesterone secretion in cultured bovine luteal cells.
Inhibitory effects of gossypol on the female reproductive system have been recently reported. This study investigated a possible site of gossypol action on progesterone synthesis. Bovine luteal cells were cultured with hCG and forskolin in the presence or absence of gossypol. At 10 micrograms/ml, gossypol significantly inhibited hCG- and forskolin-stimulated progesterone secretion and intracellular cAMP formation; at 20 micrograms/ml, gossypol completely abolished the stimulative effect of hCG and forskolin. The results suggest that adenylate cyclase may be a site of gossypol action on steroidogenesis of bovine luteal cells.
Gu
Y
Y
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Ohio State University, Columbus 43210-1092.
Chang
C J
CJ
Rikihisa
Y
Y
Lin
Y C
YC
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D005576:Colforsin / Q000494:pharmacology; D003338:Corpus Luteum / Q000187:drug effects*; D000242:Cyclic AMP / Q000096:biosynthesis; D005260:Female; D006072:Gossypol / Q000494:pharmacology*; D066298:In Vitro Techniques; D008184:Luteal Cells / Q000187:drug effects* / Q000378:metabolism; D008183:Luteal Phase; D011374:Progesterone / Q000378:metabolism*
D006063:Chorionic Gonadotropin; D005576:Colforsin; D011374:Progesterone; D000242:Cyclic AMP; D006072:Gossypol