Effect of streptococcal pyrogenic exotoxin on rabbit macrophage functions in vitro: mediation by splenic lymphocytes.
Streptococcal pyrogenic exotoxin (SPE) showed no direct effect on rabbit macrophage functions in vitro. However, when splenic lymphocytes were added to macrophage cultures, SPE caused marked augmentation of glucose consumption and superoxide anion production, and concomitant inhibition of phagocytosis without loss of cell viability. The SPE effects were demonstrated to be mediated by a soluble factor(s) released from the splenic lymphocytes in response to SPE stimulus.
Murai
T
T
National Institute of Hygienic Sciences, Osaka Branch, Japan.
Ogawa
Y
Y
Kawasaki
H
H
D000818:Animals; D001426:Bacterial Proteins; D002470:Cell Survival; D002478:Cells, Cultured; D005098:Exotoxins / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D008214:Lymphocytes / Q000502:physiology*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D008565:Membrane Proteins; D010529:Peritoneal Cavity / Q000166:cytology; D010587:Phagocytosis; D011817:Rabbits; D013154:Spleen / Q000166:cytology; D013291:Streptococcus; D013481:Superoxides / Q000378:metabolism
D001426:Bacterial Proteins; D005098:Exotoxins; D008565:Membrane Proteins; C117980:SpeA protein, Streptococcus pyogenes; C027951:erythrogenic toxin; D013481:Superoxides; D005947:Glucose