title: Mycoplasmas produce double-stranded ribonuclease.
abstract: Mycoplasmas (Mollicutes) constitute a constant threat as insidious contaminants of animal cell cultures. They are responsible for myriad biochemical reactions associated with the cells they infect, and undoubtedly have been the source of metabolic and physiological activities attributed to their hosts. In an attempt to demonstrate a dsRNA-inducible double-stranded ribonuclease (dsRNase) in mammalian cells, comparable to that reported in avian cells, we discovered high levels of dsRNase "induced" by a particular stock of vesicular stomatitis virus. We now report that the double-stranded ribonuclease resulted from the activity of a contaminant in that stock--a "noncultivable" Mycoplasma hyorhinis. This report demonstrates the ubiquitous distribution of dsRNase among mycoplasmas, presents some characteristics of the enzyme and its production, and implicates once again mycoplasmas as contaminants of cell culture and potential perturbers of cellular physiology.
authors.affiliation: Department of Molecular and Cell Biology, University of Connecticut, Storrs 06269-3044.
authors.forename: P I
authors.initials: PI
authors.lastname: Marcus
authors.forename: I
authors.initials: I
authors.lastname: Yoshida
mesh_terms: D000818:Animals; D002478:Cells, Cultured / Q000382:microbiology; D009174:Mycoplasma / Q000201:enzymology*; D012260:Ribonucleases / Q000378:metabolism*; D014721:Vesicular stomatitis Indiana virus / Q000502:physiology
chemical_list: D012260:Ribonucleases