Interaction of vasopressin and oxytocin with human breast carcinoma cells.
The arginine vasopressin and oxytocin content of normal and cancerous human breast tissue were measured using radioimmunoassay. Both peptides were present in amounts greater than that found in the circulation, but no difference between normal and malignant tissues was found. Binding of [3H]oxytocin and [3H]vasopressin were characterized in human breast carcinoma cells (MCF7 cells). Binding of both hormones to MCF7 cells was specific and saturable, the vasopressin receptor found to be of the V1 subtype. Scatchard analyses of the data were linear, indicating a single high affinity, low capacity binding site for each hormone (vasopressin: KD = 47.4 +/- 1.6 nmol/liter, Bmax = 27,300 +/- 6,500 sites/cell; oxytocin: KD = 51.3 +/- 0.4 nmol/liter, Bmax = 87,000 +/- 4,000 sites/cell). The effects of vasopressin and oxytocin on the growth of MCF7 cells were assessed using protein accumulation and cell numbers. Vasopressin at 10-1000 pmol/liter was mitogenic for MCF7 cells, but higher doses (10 nmol/liter) were growth inhibitory. Oxytocin was also mitogenic for MCF7 cells but to a lesser extent than vasopressin. In conclusion, we suggest that vasopressin and possibly oxytocin may be important modulators of the growth of some human breast carcinomas.
Taylor
A H
AH
Division of Biochemical Medicine, St. George's Hospital Medical School, London, England.
Ang
V T
VT
Jenkins
J S
JS
Silverlight
J J
JJ
Coombes
R C
RC
Luqmani
Y A
YA
D001127:Arginine Vasopressin / Q000032:analysis* / Q000378:metabolism; D001667:Binding, Competitive; D001940:Breast / Q000737:chemistry / Q000378:metabolism*; D001943:Breast Neoplasms / Q000737:chemistry / Q000378:metabolism*; D002460:Cell Line; D005260:Female; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism; D007700:Kinetics; D010121:Oxytocin / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology; D011863:Radioimmunoassay; D011945:Receptors, Angiotensin / Q000378:metabolism*; D018045:Receptors, Oxytocin; D017483:Receptors, Vasopressin; D012016:Reference Values; D014667:Vasopressins / Q000378:metabolism / Q000494:pharmacology
D007295:Inositol Phosphates; D011945:Receptors, Angiotensin; D018045:Receptors, Oxytocin; D017483:Receptors, Vasopressin; D014667:Vasopressins; D001127:Arginine Vasopressin; D010121:Oxytocin