title: The effect of novobiocin on yeast topoisomerase type II.
abstract: Low concentrations of novobiocin are toxic to permeable yeast cells, but do not inhibit type II topoisomerase activity. Furthermore, the enzyme does not bind specifically to novobiocin-Sepharose. These observations are in agreement with genetical analyses. Mutations at a single locus that confer novobiocin resistance and temperature sensitivity exhibit a similar phenotype to cells treated with novobiocin, but are not topoisomerase II mutants.
authors.affiliation: Department of Genetics, University of Leicester, England.
authors.forename: M J
authors.initials: MJ
authors.lastname: Pocklington
authors.forename: J R
authors.initials: JR
authors.lastname: Jenkins
authors.forename: E
authors.initials: E
authors.lastname: Orr
mesh_terms: D002846:Chromatography, Affinity; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D004352:Drug Resistance, Microbial; D004591:Electrophoresis, Polyacrylamide Gel; D008856:Microscopy, Fluorescence; D009154:Mutation; D009675:Novobiocin / Q000378:metabolism / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D013696:Temperature
chemical_list: D009675:Novobiocin; D004250:DNA Topoisomerases, Type II