Cloning and expression of Thiobacillus versutus cryptic plasmid pTAV-1 DNA in Escherichia coli.
pTAV-1 is an approximately 100 kb Thiobacillus versutus cryptic plasmid. pTAV-1 DNA was cloned in Escherichia coli. Nine recombinant plasmids containing pTAV-1 DNA inserted into the EcoRI restriction site of pACYC184 were constructed. The origin of DNA inserts was confirmed by Southern blot hybridization. The expression of mixotrophic T. versutus plasmid genes was demonstrated in E. coli.
Jagusztyn-Krynicka
E K
EK
Institute of Microbiology, University of Warsaw, Poland.
Bielecki
J
J
Kauc
B
B
D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D015246:Deoxyribonuclease EcoRI; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000235:genetics*; D015183:Restriction Mapping; D013855:Thiobacillus / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D015246:Deoxyribonuclease EcoRI