Changes in lipid ordering of model phospholipid membranes treated with chrysotile and crocidolite asbestos.
A variety of inorganic particles bind to cell membranes and cause alterations in phagocytosis, migration, and metabolism leading to eventual cell death. The mechanism which mediate these events at the complex membrane level are not known. In attempting to define the mechanisms of asbestos-induced membrane changes, we have prepared small unilamellar vesicles (SUV) of dipalmitoyl phosphatidylcholine as a simplified model of the cell membrane. Negatively stained preparations for electron microscopy demonstrated that the SUVs bind to both chrysotile and crocidolite asbestos fibers. Electron spin resonance showed that both asbestos types caused increased membrane rigidity at the level of the 12th carbon, whereas chrysotile induced increased rigidity at the 5th carbon level as well. Studies using DPPC membranes compared to SUVs made of phosphatidylcholine from egg yolk support the view that lipid peroxidation may play no significant role in alterations of membrane rigidity induced by the asbestos particle binding. Similar alterations of membrane rigidity and lipid peroxidation at the depth of the 12th carbon have been reported in complex naturally occurring erythrocyte membranes. This suggests that our observations may be relevant to biological membranes and that the model system of SUVs is appropriate for additional studies on the mechanisms of particle-induced membrane injury.
Gendek
E G
EG
Laboratory of Pulmonary Pathobiology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
Brody
A R
AR
D000818:Animals; D001194:Asbestos / Q000009:adverse effects*; D017638:Asbestos, Crocidolite; D017632:Asbestos, Serpentine; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000648:ultrastructure; D015227:Lipid Peroxidation / Q000187:drug effects; D008081:Liposomes / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism*; D008854:Microscopy, Electron; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats
D017632:Asbestos, Serpentine; D008081:Liposomes; D008563:Membrane Lipids; D010743:Phospholipids; D017638:Asbestos, Crocidolite; D001194:Asbestos