The isolation of the acid phosphatase-1 gene of Drosophila melanogaster and a chromosomal breakpoint inducing its position effect variegation.
Over 120 kb of contiguous genomic DNA sequence derived from the 99C-99D region of the Drosophila melanogaster third chromosome were isolated by molecular cloning. Sequences within this region required for the expression of the lysosomal gene-enzyme system acid phosphatase-1 (Acph-1) were identified by both P element-mediated germline transformation and transient expression and lie within a single 5 kb fragment. Acph-1 is encoded by a 2.1 kb poly(A)+ RNA transcript, which is expressed throughout development. Enzyme activity peaks also correlate with increases in RNA abundance. The ca-74 deletion, which exhibits position effect variegation at the Acph-1 gene (Frisardi and MacIntyre 1984), was also partially characterized. The variegating ca-74 breakpoint is located approximately 20 kb proximal to the Acph-1 gene. Results suggest that the heterochromatin at this breakpoint comprises highly repetitive or satellite DNA.
Shaffer
C D
CD
Section of Genetics and Development, Cornell University, Ithaca, NY 14853.
MacIntyre
R J
RJ
D000135:Acid Phosphatase / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D002875:Chromosomes / Q000378:metabolism*; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D015870:Gene Expression; D005796:Genes; D008247:Lysosomes / Q000201:enzymology; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D004251:DNA Transposable Elements; D004247:DNA; D000135:Acid Phosphatase