Cellular distribution of the hamster liver specific nucleolar antigens.
The immunochemical localization of hamster liver nucleolar antigens in subcellular fractions (nuclei, 10,000 x g pellet, 100,000 x g pellet and supernatant), nuclear substructures (chromatin, nuclear matrix, nuclear envelope, nucleoli, RNP particles and nucleosomes), and three classes of nonhistone chromosomal proteins with different affinities to DNA (NHCP1, NHCP2 and NHCP3) from nuclease-sensitive and nuclease-resistant chromatin fractions of hamster liver were studied. Six main nucleolar antigens with mol. wts 27,000; 29,000; 30,000; 36,000; 45,000; and 46,000 were found in subcellular fractions, nuclear substructures and classes of non-histone proteins of hamster liver. The antigens with mol.wts of approx. 27,000; 29,000; and 36,000 which were absent in hamster pancreas, spleen and Kirkman--Robbins hepatoma nuclei, seem specific for liver tissue.
Wojtkowiak
Z
Z
Department of Cytobiochemistry, University of Lodz, Poland.
Lipińska
A
A
Kłyszejko-Stefanowicz
L
L
D000818:Animals; D000941:Antigens / Q000032:analysis*; D002458:Cell Fractionation; D002466:Cell Nucleolus / Q000032:analysis / Q000648:ultrastructure*; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D007124:Immunoenzyme Techniques; D008099:Liver / Q000032:analysis / Q000648:ultrastructure*; D008114:Liver Neoplasms, Experimental / Q000032:analysis / Q000648:ultrastructure; D008647:Mesocricetus; D008970:Molecular Weight; D015530:Nuclear Matrix / Q000032:analysis / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000032:analysis*
D000941:Antigens; D009687:Nuclear Proteins