title: Inhibitory effect of local anesthetics on Na+/H+ antiporter in brush border membrane-reconstituted vesicles.
abstract: The effects of three local anesthetics, lidocaine, dibucaine, and tetracaine, on Na+/H+ antiporter activity were examined in brush border membrane-reconstituted vesicles. Lidocaine at 10 microM inhibited H+ efflux in the presence of an inward Na+ gradient, suggesting that this anesthetic specifically inhibits the Na+/H+ antiporter. On the other hand, dibucaine and tetracaine decreased H+ efflux even in the absence of a Na+ gradient.
authors.affiliation: Department of Biochemistry, Faculty of Pharmaceutical Sciences, Setsunan University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Shibamoto
authors.forename: T
authors.initials: T
authors.lastname: Hori
authors.forename: M
authors.initials: M
authors.lastname: Hayakawa
authors.forename: M
authors.initials: M
authors.lastname: Nango
authors.forename: E J
authors.initials: EJ
authors.lastname: Cragoe
authors.forename: N
authors.initials: N
authors.lastname: Oku
authors.forename: F
authors.initials: F
authors.lastname: Ito
mesh_terms: D000779:Anesthetics, Local / Q000494:pharmacology*; D000818:Animals; D017920:Antiporters; D002352:Carrier Proteins / Q000187:drug effects*; D003992:Dibucaine / Q000494:pharmacology; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008012:Lidocaine / Q000494:pharmacology; D008871:Microvilli / Q000187:drug effects* / Q000378:metabolism; D011522:Protons; D051381:Rats; D012964:Sodium / Q000378:metabolism*; D013748:Tetracaine / Q000494:pharmacology
chemical_list: D000779:Anesthetics, Local; D017920:Antiporters; D002352:Carrier Proteins; D011522:Protons; D013748:Tetracaine; D008012:Lidocaine; D012964:Sodium; D003992:Dibucaine