pSV3neo transfection and radiosensitivity of human cancer cell lines.
Immortalisation of human fibroblasts by transfection with a plasmid, pSV3neo, results in an increase in their radioresistance. The change in radiosensitivity may either be a consequence of transformation or due to expression of the SV40 T-antigen in pSV3neo. To investigate these two possibilities, we transfected pSV3neo into cells already transformed and immortalised. The radiosensitivities of three human bladder cancer cell lines were unaltered in clones expressing T-antigen, indicating that the changes observed in fibroblasts probably are a consequence of transformation, and not the presence of SV40 T-antigen.
Parris
C N
CN
Department of Urological Oncology, University College London, St. Paul's Hospital, U.K.
Masters
J R
JR
Green
M H
MH
D000957:Antigens, Viral, Tumor / Q000235:genetics; D002999:Clone Cells / Q000502:physiology; D004279:DNA, Viral / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D010957:Plasmids / Q000235:genetics; D011836:Radiation Tolerance / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000532:radiotherapy
D000957:Antigens, Viral, Tumor; D004279:DNA, Viral