title: Monoclonal antibody to type F Clostridium botulinum toxin.
abstract: Hybridomas synthesizing monoclonal antibodies (MAbs) against type F Clostridium botulinum toxin were developed. MAb from one stable hybridoma, hybridoma 223, consisted of kappa light chains and an immunoglobulin G subclass 2a heavy chain. This MAb was used in a double-sandwich enzyme-linked immunosorbent assay to detect type F toxin in foods, culture fluids, and purified toxin preparations. The sensitivity of the double-sandwich enzyme-linked immunosorbent assay was approximately 10 mouse lethal doses of toxin per ml of toxic fluid.
authors.affiliation: Food and Drug Administration, Atlanta, Georgia 30309.
authors.forename: J L
authors.initials: JL
authors.lastname: Ferreira
authors.forename: M K
authors.initials: MK
authors.lastname: Hamdy
authors.forename: S G
authors.initials: SG
authors.lastname: McCay
authors.forename: F A
authors.initials: FA
authors.lastname: Zapatka
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001905:Botulinum Toxins / Q000032:analysis / Q000276:immunology*; D003014:Clostridium botulinum / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005506:Food Contamination / Q000032:analysis; D006825:Hybridomas / Q000276:immunology; D051379:Mice
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001905:Botulinum Toxins