Translational motion of actin filaments in the presence of heavy meromyosin and MgATP as measured by Doppler broadening of laser light scattering.
Intensity fluctuations of laser light scattering were utilized in order to follow enhancement of translational motion of the actin-heavy meromyosin (HMM) complex in extremely dilute solutions accompanied by the hydrolysis of MgATP. Such enhancement was anticipated on the basis of the idea that active streaming along actin filaments should be associated with their mechanochemical reactivity. Native tropomyosin was added in order to stabilize actin in its filamentous form, thus allowing the reduction of actin concentration below 50 micrograms/ml to enable free movement of neighboring filaments and yet give a reliable signal. Analysis of the data in terms of Doppler broadening led to an approximate evaluation of the average velocity of translation of the mobile filaments. This velocity was found to increase with increasing HMM concentration up to a maximum attained at a molar ratio HMM/actin of 1:2, and then decreased. Total intensity measurements indicate that the mobile scatterer is actually a complex of HMM with an isolated actin filament. HMM subfragment-1 was found to be ineffective. These results suggest that cooperation between the two myosin heads is necessary for efficient induction of active streaming along isolated actin filaments.
Tirosh
R
R
Department of Polymer Research, Weizmann Institute of Science, Rehovot, Israel.
Low
W Z
WZ
Oplatka
A
A
D000199:Actins / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D055598:Chemical Phenomena; D002621:Chemistry; D006868:Hydrolysis; D007834:Lasers; D046911:Macromolecular Substances; D015879:Myosin Subfragments / Q000378:metabolism*; D009994:Osmolar Concentration; D012542:Scattering, Radiation; D012996:Solutions; D014335:Tropomyosin / Q000378:metabolism
D000199:Actins; D046911:Macromolecular Substances; D015879:Myosin Subfragments; D012996:Solutions; D014335:Tropomyosin; D000255:Adenosine Triphosphate; D002118:Calcium