Plasmid transformation of Clostridium perfringens by electroporation methods.
Two electroporation methods were compared and modified to improve the frequencies of transfer of plasmid DNA into Clostridium perfringens. A plasmid shuttle vector, pSB92A2, containing chloramphenicol and ampicillin resistance genes and a clostridial origin of replication isolated from a cryptic C. perfringens plasmid, was constructed and successfully introduced into C. perfringens by both electrotransformation methods. Modifications which improved frequencies by 15-28 fold are described and may improve frequencies sufficiently for some vector/host combinations to consider the future use of more direct cloning strategies for the clostridia.
Phillips-Jones
M K
MK
Department of Applied Biochemistry and Food Science, University of Nottingham, Loughborough, Leics., U.K.
D003016:Clostridium perfringens / Q000235:genetics*; D004560:Electricity; D004926:Escherichia coli / Q000235:genetics; D005821:Genetic Techniques; D005822:Genetic Vectors; D010957:Plasmids; D014170:Transformation, Genetic