title: Receptors for insulin and IGF-I in bovine lens epithelium.
abstract: The presence of insulin receptors has not yet been established in lens epithelial cells. Our goal was to understand the involvement of insulin in lens pathology, especially diabetic cataractogenesis. As a first step in this process, we evaluated the presence of insulin receptors in bovine lens epithelium. Insulin bound to insulin receptor with a high affinity (KD 7.0 nM), although this binding of insulin to its receptor could be replaced by IGF-I (KD 41 nM). Similarly IGF-I binding to IGF-I receptor (KD 0.38 nM) could be inhibited by insulin (KD 53.7 nM). SDS-PAGE and subsequent autoradiography of affinity labeled insulin receptor show the presence of a 130 kDa band, indicating that the lens epithelium insulin receptor may be similar to insulin receptors isolated from other tissues.
authors.affiliation: Department of Human Biological Chemistry & Genetics, University of Texas Medical Branch, Galveston 77550.
authors.forename: A C
authors.initials: AC
authors.lastname: Sinha
authors.forename: C A
authors.initials: CA
authors.lastname: Stuart
authors.forename: S K
authors.initials: SK
authors.lastname: Srivastava
mesh_terms: D000345:Affinity Labels; D000818:Animals; D001345:Autoradiography; D002417:Cattle; D002462:Cell Membrane / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004848:Epithelium / Q000737:chemistry; D007908:Lens, Crystalline / Q000737:chemistry*; D011869:Radioligand Assay; D011972:Receptor, Insulin / Q000032:analysis*; D011956:Receptors, Cell Surface / Q000032:analysis*; D017451:Receptors, Somatomedin
chemical_list: D000345:Affinity Labels; D011956:Receptors, Cell Surface; D017451:Receptors, Somatomedin; D011972:Receptor, Insulin