Procaine inhibits cyclic AMP-induced steroidogenesis in isolated bovine adrenocortical cells.
Effects of procaine on dibutyryl adenosine 3',5'-cyclic monophosphate ((Bu)2cAMP)-, Ca2(+)- or forskolin-induced steroidogenesis were examined in isolated bovine adrenocortical cells. Procaine (less than 1.0 mM) caused a marked suppression of (Bu)2cAMP- or forskolin-induced steroidogenesis in the absence of extracellular Ca2+, but did not affect on Ca2(+)-induced steroidogenesis in the cells. (Bu)2cAMP decreased the cell associated 45Ca2+. However, procaine (300 microM) inhibited this effect of (Bu)2cAMP. These results suggest that procaine may abolish (Bu)2cAMP-induced Ca2+ release from intracellular calcium store(s) and inhibits steroidogenesis.
Noguchi
A
A
Department of Pharmacology I, Jikei University School of Medicine, Tokyo, Japan.
Takamura
M
M
Yamada
K
K
Tou
S
S
Kawamura
M
M
D000302:Adrenal Cortex / Q000166:cytology / Q000378:metabolism*; D000305:Adrenal Cortex Hormones / Q000096:biosynthesis; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002132:Calcium Radioisotopes; D002417:Cattle; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D066298:In Vitro Techniques; D011343:Procaine / Q000494:pharmacology*; D013256:Steroids / Q000096:biosynthesis*
D000305:Adrenal Cortex Hormones; D002132:Calcium Radioisotopes; D013256:Steroids; D005576:Colforsin; D011343:Procaine; D003994:Bucladesine; D000242:Cyclic AMP