title: Crystallization of recombinant rat cathepsin B.
abstract: A glycosylation-minus mutant of rat cathepsin B expressed in yeast has been purified and crystallized. X-ray diffraction data have been collected and molecular replacement for solving the structure is in progress. The space group for the recombinant rat cathepsin B was determined to be P2(1) with unit cell dimensions alpha = 62.2 A, b = 90.19 A, c = 47.07 A, and beta = 97.43 degrees. A unit cell contains 4 molecules and 2 molecules per asymmetric unit.
authors.affiliation: Division of Biological Sciences, National Research Council of Canada, Ottawa, Ontario.
authors.forename: X
authors.initials: X
authors.lastname: Lee
authors.forename: F R
authors.initials: FR
authors.lastname: Ahmed
authors.forename: T
authors.initials: T
authors.lastname: Hirama
authors.forename: C P
authors.initials: CP
authors.lastname: Huber
authors.forename: D R
authors.initials: DR
authors.lastname: Rose
authors.forename: R
authors.initials: R
authors.lastname: To
authors.forename: S
authors.initials: S
authors.lastname: Hasnain
authors.forename: A
authors.initials: A
authors.lastname: Tam
authors.forename: J S
authors.initials: JS
authors.lastname: Mort
mesh_terms: D000818:Animals; D002401:Cathepsin B / Q000235:genetics / Q000302:isolation & purification*; D003001:Cloning, Molecular; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D051381:Rats; D011994:Recombinant Proteins / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D002401:Cathepsin B