Changes in the expression of elastase and cathepsin B with differentiation of U937 promonocytes by GMCSF.
The human promonocytic cell line, U937, when treated for up to 72h with 12,O,tetradecanoyl-phorbol-13-acetate or granulocyte-macrophage colony-stimulating factor, exhibited increased phagocytic activity and expression of the marker p150/95. There was an associated increase in the monocyte proteinase cathepsin B and its mRNA but decreased cellular levels of neutrophil elastase and elastase mRNA. Granulocyte-macrophage colony-stimulating factor therefore causes differentiation of U937 cells, with appropriate effects on the synthesis of leukocyte proteinases.
Ward
C J
CJ
General Hospital, Birmingham, England, U.K.
Crocker
J
J
Chan
S J
SJ
Stockley
R A
RA
Burnett
D
D
D002401:Cathepsin B / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D016403:Lymphoma, Large B-Cell, Diffuse; D010196:Pancreatic Elastase / Q000235:genetics* / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000201:enzymology
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D012333:RNA, Messenger; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D010196:Pancreatic Elastase; D002401:Cathepsin B; D013755:Tetradecanoylphorbol Acetate