Inhibition of Cl(-)-stimulated ATPase activity in isolated basolateral membranes from Aplysia gut.
A Cl(-)-stimulated ATPase activity, which is sensitive to both N-ethylmaleimide and p-chloromercuribenzenesulfonate, has been localized to the basolateral membrane of Aplysia enterocytes. Dithiothreitol reversed the inhibition of Cl(-)-stimulated ATPase induced by p-chloromercuribenzenesulfonate. These results suggest that surface sulfhydryl ligands of the ATPase participate in the catalytic activity of the enzyme.
Gerencser
G A
GA
Department of Physiology, College of Medicine, JHMHC, University of Florida, Gainesville 32610.
D002731:4-Chloromercuribenzenesulfonate / Q000494:pharmacology; D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D001048:Aplysia; D001485:Basement Membrane / Q000201:enzymology; D017301:Ca(2+) Mg(2+)-ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism; D002118:Calcium / Q000494:pharmacology*; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D004229:Dithiothreitol; D005033:Ethylmaleimide / Q000494:pharmacology; D007422:Intestines / Q000201:enzymology*; D013438:Sulfhydryl Compounds
D013438:Sulfhydryl Compounds; D002731:4-Chloromercuribenzenesulfonate; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases; D005033:Ethylmaleimide; D002118:Calcium; D004229:Dithiothreitol