Light and electron microscopical immunocytochemical localization of pancreastatin-like immunoreactivity in porcine tissues.
Pancreastatin is a 49 amino acid comprising peptide isolated from porcine pancreas that is derived by proteolytic processing from chromogranin A. Using an antibody against the synthetic C-terminal fragment pancreastatin (33-49), we examined the light and electron microscopical immunocytochemical localization of this peptide in porcine tissues. Pancreastatin-like immunoreactivity (PLI) was found in pancreatic somatostatin-, insulin- and glucagon cells in varying intensities; pancreatic polypeptide cells were always negative. At the electron microscopical (EM) level the immunoreactivity was confined to the electron dense core of the secretory granules in the case of somatostatin and insulin cells or to the less electron dense "halo" of the glucagon granules. In the antrum PLI positive cells represented gastrin (G), somatostatin (D) and enterochromaffin (EC) cells, in the duodenum in addition to EC- and G-cells a small number of PLI positive cells showed a positive immunoreaction for glucagon-like peptide (GLP) I and secretin in serial sections. Both norepinephrine and epinephrine containing cells of the adrenal medulla exhibited a strong reaction for PLI. In the pituitary several cell populations stained with varying intensities, including gonadotrophs and thyrotrophys. PLI is present in a distinct and characteristic subpopulation of neuroendocrine cells in various organs. The subcellular localization may indicate a function in the granular concentration, packaging and storage of peptides and amines in the brain-gut endocrine system.
Lamberts
R
R
Department of Internal Medicine, Georg August University of Göttingen, Federal Republic of Germany.
Schmidt
W E
WE
Creutzfeldt
W
W
D000313:Adrenal Medulla / Q000276:immunology* / Q000648:ultrastructure; D000818:Animals; D053379:Chromogranin A; D002864:Chromogranins / Q000276:immunology; D004064:Digestive System / Q000276:immunology* / Q000648:ultrastructure; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry; D007515:Islets of Langerhans / Q000276:immunology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D010187:Pancreatic Hormones / Q000276:immunology*; D010902:Pituitary Gland / Q000276:immunology* / Q000648:ultrastructure; D013552:Swine
D053379:Chromogranin A; D002864:Chromogranins; D007106:Immune Sera; D010187:Pancreatic Hormones; C050836:pancreastatin