D1 dopamine receptors in neurite regions of embryonic and differentiated retina are highly coupled to adenylate cyclase in the embryonic but not in the mature tissue.
[3H]SCH 23390 binds stereospecifically and with high affinity to D1 dopaminergic receptors in the developing chick retina. Autoradiographic experiments revealed that in retinas from 3-day-old chicken and embryos with 12, 14 and 16 days of development, specific labeling of [3H]SCH 23390 was mainly observed over the plexiform layers of the tissue, showing that dopaminergic D1 receptors are localized in retina cell neurites since the initial stages of neurite formation. The total number of [3H]SCH 23390 binding sites increased 5-fold during the differentiation of the retina, while the dopamine-dependent cyclic adenosine monophosphate (AMP) accumulation was significantly decreased. Consequently, the ratio between dopamine-dependent cyclic AMP accumulation and [3H]SCH 23390 binding sites decreased 10-fold as retina differentiated, indicating that a significant portion of D1 receptors in retinas from adult chicken are not effectively coupled to adenylate cyclase molecules.
Ventura
A L
AL
Laboratory of Neurochemistry, Instituto de Biofisica Carlos Chagas Filho, UFRJ, Rio de Janeiro, Brazil.
de Mello
F G
FG
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000375:Aging / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D001552:Benzazepines / Q000378:metabolism / Q000494:pharmacology; D002642:Chick Embryo; D000242:Cyclic AMP / Q000378:metabolism; D004298:Dopamine / Q000502:physiology; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D008566:Membranes / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D011954:Receptors, Dopamine / Q000378:metabolism*; D012160:Retina / Q000196:embryology / Q000201:enzymology / Q000378:metabolism*
D001552:Benzazepines; D007202:Indicators and Reagents; D011954:Receptors, Dopamine; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D004298:Dopamine