Beta 2-adrenoceptor up-regulation in relation to cAMP production in human lymphocytes after physical exercise.
The effect of isotonic bicycle exercise on beta 2-adrenoceptors and cAMP production in lymphocytes was studied. The origin of the newly exposed receptors was studied by measuring radioligand binding in the presence of hydrophylic or lipophylic 'cold' ligands. During exercise beta 2-adrenoceptor density increased from 1207 +/- 144 to 1776 +/- 152 sites/cell (56 +/- 14%). Isoprenaline induced an increase in cAMP synthesis, which was 68 +/- 15% higher than those values measured before the test. Forskolin did not induce a higher increase in cAMP production after exercise. The increase in receptor density seems not to be due to a transfer of internalized receptors to the membrane surface. We conclude that physical exercise induces an up-regulation of beta 2-adrenoceptors on lymphocytes and that these adrenoceptors are functional.
Graafsma
S J
SJ
Department of Medicine, University of Nijmegen, The Netherlands.
van Tits
L J
LJ
Willems
P H
PH
Hectors
M P
MP
Rodrigues de Miranda
J F
JF
De Pont
J J
JJ
Thien
T
T
D000328:Adult; D000242:Cyclic AMP / Q000096:biosynthesis*; D015444:Exercise / Q000502:physiology*; D005260:Female; D006339:Heart Rate / Q000502:physiology; D006801:Humans; D007457:Iodine Radioisotopes; D020120:Iodocyanopindolol; D008024:Ligands; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D009638:Norepinephrine / Q000097:blood; D010869:Pindolol / Q000031:analogs & derivatives; D011943:Receptors, Adrenergic, beta / Q000502:physiology*; D015854:Up-Regulation / Q000502:physiology*
D007457:Iodine Radioisotopes; D008024:Ligands; D011943:Receptors, Adrenergic, beta; D020120:Iodocyanopindolol; D010869:Pindolol; D000242:Cyclic AMP; D009638:Norepinephrine