Two-dimensional crystallization of DNA gyrase B subunit on specifically designed lipid monolayers.
The B subunit of DNA gyrase formed two-dimensional crystals when bound to a specifically recognized phospholipid spread into a monolayer at the air/water interface. The especially designed lipids consisted of novobiocin coupled through the 3' or 2" hydroxyl group and a hydrophilous linker of a given length to dioleoylphosphatidic acid. Two-dimensional crystals of the gyrase B subunit are formed under physiological conditions of pH and ionic strength, with no precipitant added to the solution. Crystal diffraction extended to a 2.7 nm resolution in negative stain, with unit cell parameters a = 6.1 nm, b = 7.6 nm and gamma = 64 degrees.
Lebeau
L
L
Laboratoire de Synthèse Bio-organique, CNRS, Unité de Recherche Associée 1386, Faculté de Pharmacie, Illkirch, France.
Regnier
E
E
Schultz
P
P
Wang
J C
JC
Mioskowski
C
C
Oudet
P
P
D001419:Bacteria; D003432:Cross-Linking Reagents; D004250:DNA Topoisomerases, Type II; D007091:Image Processing, Computer-Assisted; D009675:Novobiocin; D010712:Phosphatidic Acids; D010743:Phospholipids; D011487:Protein Conformation
D003432:Cross-Linking Reagents; D010712:Phosphatidic Acids; D010743:Phospholipids; C037657:dioleoylphosphatidic acid; D009675:Novobiocin; D004250:DNA Topoisomerases, Type II