Dot immunobinding assay in comparison with enzyme-linked immunosorbent assay for the detection of bluetongue virus antibodies in sheep.
A total of 384 sheep serum samples collected from two organised sheep farms was tested by dot immunobinding assay (DIA) and indirect enzyme-linked immunosorbent assay (I-ELISA) for the presence of bluetongue virus (BTV) antibodies. The results of both these assays were compared to find a sensitive, specific, rapid, easily performed and economical test for the diagnosis of bluetongue disease. DIA detected BTV antibodies in 210 samples (54.94%) and I-ELISA detected 157 positive samples (40.88%). Competitive ELISA (C-ELISA) was performed to check the discrepancies in I-ELISA and DIA. On the basis of these tests the overall agreement, relative specificity and sensitivity between ELISA and DIA were 75%, 87.6% and 100%, respectively. DIA was found to be a rapid, sensitive, easily performed and economical test as compared to ELISA.
Gupta
Y
Y
Department of Veterinary Microbiology, College of Veterinary Sciences, Haryana Agricultural University, India.
Chand
P
P
Singh
A
A
Jain
N C
NC
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D001667:Binding, Competitive; D001819:Bluetongue / Q000175:diagnosis*; D001820:Bluetongue virus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D015151:Immunoblotting; D011237:Predictive Value of Tests; D012087:Reoviridae / Q000276:immunology*; D015203:Reproducibility of Results; D012756:Sheep
D000914:Antibodies, Viral