title: Use of positive ion fast atom bombardment mass spectrometry for rapid identification of a bile alcohol glucuronide isolated from cerebrotendinous xanthomatosis patients.
abstract: The identification of a major biliary and plasma bile alcohol glucuronide, 5 beta-cholestane-3 alpha, 7 alpha, 12 alpha, 25-tetrol-3-0-beta-D-glucuronide, present in cerebrotendinous xanthomatosis (CTX) patients, was investigated by positive ion fast atom bombardment mass spectrometry (FAB-MS). The spectrum was characterized by abundant ions formed by attachment of a proton, [M + H]+, or of alkali ions, [M + Na]+ and [M + 39K]+, to the glucuronide salt. These ions allowed an unambiguous deduction of the molecular weight of the sample. It is suggested that FAB-MS could be used in the rapid diagnosis of CTX.
authors.affiliation: Department of Medicine, UMDNJ-New Jersey Medical School, Newark.
authors.forename: B
authors.initials: B
authors.lastname: Dayal
authors.forename: G
authors.initials: G
authors.lastname: Salen
authors.forename: G S
authors.initials: GS
authors.lastname: Tint
authors.forename: S
authors.initials: S
authors.lastname: Shefer
authors.forename: S W
authors.initials: SW
authors.lastname: Benz
mesh_terms: D001649:Bile Duct Diseases / Q000175:diagnosis*; D055598:Chemical Phenomena; D002621:Chemistry; D002777:Cholestanols / Q000032:analysis* / Q000097:blood; D004583:Electrons; D005965:Glucuronates / Q000032:analysis* / Q000097:blood; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D012680:Sensitivity and Specificity; D014973:Xanthomatosis / Q000175:diagnosis*
chemical_list: D002777:Cholestanols; D005965:Glucuronates