Interferon suppresses sympathetic neuronal cell death caused by nerve growth factor deprivation.
Cultured rat sympathetic neurons die within 48 h after being deprived of nerve growth factor. Addition of interferons (IFN-alpha/beta or IFN-gamma) prevented the cell death in a dose-dependent manner. Upon longer periods of nerve growth factor deprivation, IFNs failed to maintain survival. Thus, IFNs retarded neuronal death, but did not prevent it. Ligand binding, autoradiography, and cross-linking experiments demonstrated the presence of specific IFN-gamma receptors on sympathetic neurons similar to those seen on other cell types. The possible relationships of the death-suppressing actions of IFNs are compared to the mechanisms of the antiviral or antiproliferative actions of IFNs.
Chang
J Y
JY
Department of Pharmacology, Washington University School of Medicine, St. Louis, Missouri 63110.
Martin
D P
DP
Johnson
E M
EM
D000263:Adenylate Kinase / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002470:Cell Survival; D002478:Cells, Cultured; D005728:Ganglia, Sympathetic / Q000166:cytology* / Q000196:embryology; D007370:Interferon Type I / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D009414:Nerve Growth Factors / Q000276:immunology / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism; D011971:Receptors, Immunologic / Q000378:metabolism; D017471:Receptors, Interferon; D017475:Receptors, Nerve Growth Factor; D011994:Recombinant Proteins; D013997:Time Factors
D000911:Antibodies, Monoclonal; D007370:Interferon Type I; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D017475:Receptors, Nerve Growth Factor; D011994:Recombinant Proteins; D007371:Interferon-gamma; D000263:Adenylate Kinase