title: Expression of the yeast DNA primase gene, PRI1, is regulated within the mitotic cell cycle and in meiosis.
abstract: Mitotic cultures synchronised either by a feed-starve protocol or by elutriation have been used to show that the Saccharomyces cerevisiae DNA primase I gene is periodically expressed in the cell cycle. The transcript increases many-fold in late G1 and reaches a peak at the same time as four other genes essential for DNA synthesis, CDC8, CDC9, CDC21 and POL1. The primase I transcript is also regulated in meiosis, reaching maximal levels during premeiotic DNA synthesis.
authors.affiliation: Laboratory of Cell Propagation, National Institute for Medical Research, Mill Hill, London, UK.
authors.forename: L H
authors.initials: LH
authors.lastname: Johnston
authors.forename: J H
authors.initials: JH
authors.lastname: White
authors.forename: A L
authors.initials: AL
authors.lastname: Johnson
authors.forename: G
authors.initials: G
authors.lastname: Lucchini
authors.forename: P
authors.initials: P
authors.lastname: Plevani
mesh_terms: D019915:DNA Primase; D004261:DNA Replication; D004271:DNA, Fungal / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008540:Meiosis / Q000235:genetics*; D008938:Mitosis / Q000235:genetics*; D012316:RNA Nucleotidyltransferases / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D004271:DNA, Fungal; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases