Complementary oligonucleotide sequence inhibits both Vmw65 gene expression and replication of herpes simplex virus.
The virion tegument protein, Vmw65, of herpes simplex virus is a transacting molecule which induces immediate early gene transcription. We show that an oligodeoxyribonucleotide which is complementary to the translation initiation region of Vmw65 mRNA inhibited the expression of Vmw65 biological activity in a Vmw65-expressing cell line and reduced the yield of HSV-1 in tissue culture. The levels of oligomer required to effect viral replication resulted in no observable cellular toxicity.
Draper
K G
KG
Department of Antiviral Chemotherapy, Schering-Plough Corporation, Bloomfield, NJ 07003.
Ceruzzi
M
M
Kmetz
M E
ME
Sturzenbecker
L J
LJ
D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D001483:Base Sequence; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D006822:Hybrid Cells; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D015183:Restriction Mapping; D018139:Simplexvirus / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D015534:Trans-Activators / Q000096:biosynthesis / Q000235:genetics*; D014779:Virus Replication / Q000187:drug effects*; D001616:beta-Galactosidase / Q000096:biosynthesis
D000998:Antiviral Agents; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D015534:Trans-Activators; D001616:beta-Galactosidase