Newcastle disease virus fusion protein expressed in a fowlpox virus recombinant confers protection in chickens.
A cDNA copy of the RNA encoding the fusion (F) protein of Newcastle disease virus (NDV) strain Texas, a velogenic strain of NDV, was obtained and the sequence was determined. The 1,792-base-pair sequence encodes a protein of 553 amino acids which has essential features previously established for the F protein of virulent NDV strains. These include the presence of three strongly hydrophobic regions and pairs of dibasic amino acids in the pentapeptide Arg-Arg-Gln-Arg-Arg preceding the putative cleavage site. When inserted into a fowlpox virus vector, a glycosylated protein was expressed and presented on the surface of infected chicken embryo fibroblast cells. The F protein expressed by the recombinant fowlpox virus was cleaved into two polypeptides. When inoculated into susceptible birds by a variety of routes, an immunological response was induced. Ocular or oral administration of the recombinant fowlpox virus gave partial protection, whereas both intramuscular and wing-web routes of inoculation gave complete protection after a single inoculation.
Taylor
J
J
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201.
Edbauer
C
C
Rey-Senelonge
A
A
Bouquet
J F
JF
Norton
E
E
Goebel
S
S
Desmettre
P
P
Paoletti
E
E
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000276:immunology*; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D005455:Fluorescent Antibody Technique; D005587:Fowlpox virus / Q000235:genetics*; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000517:prevention & control*; D009522:Newcastle disease virus / Q000235:genetics / Q000276:immunology*; D011233:Precipitin Tests; D014614:Vaccines, Synthetic; D014760:Viral Fusion Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines
D004279:DNA, Viral; D014614:Vaccines, Synthetic; D014760:Viral Fusion Proteins; D014765:Viral Vaccines