A recombinant fowlpox virus expressing the hemagglutinin-neuraminidase gene of Newcastle disease virus (NDV) protects chickens against challenge by NDV.
The hemagglutinin-neuraminidase (HN) gene from the Beaudette C strain of Newcastle disease virus (NDV) has been expressed in a recombinant fowlpox virus vector. The HN gene, under the control of the vaccinia p7.5 promoter, was inserted into a nonessential gene in the terminal inverted repeats of fowlpox virus. Expression was demonstrated in tissue culture, a protein of the correct size for fully glycosylated HN protein being recognized by an HN-specific monoclonal antibody on Western blots. When the recombinant fowlpox virus was inoculated into chickens by intravenous or wing-web routes, antibody which recognizes HN from purified NDV virions was produced. Protective immunity to NDV was generated in the chickens; at the highest dose of vaccine 100% of the chickens tested were protected against challenge with a virulent strain of NDV.
Boursnell
M E
ME
Institute for Animal Health, Houghton Laboratory, Huntingdon, Cambridgeshire, United Kingdom.
Green
P F
PF
Samson
A C
AC
Campbell
J I
JI
Deuter
A
A
Peters
R W
RW
Millar
N S
NS
Emmerson
P T
PT
Binns
M M
MM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002645:Chickens / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D003001:Cloning, Molecular; D046508:Culture Techniques; D005587:Fowlpox virus / Q000235:genetics*; D005814:Genes, Viral; D005822:Genetic Vectors; D015707:HN Protein / Q000096:biosynthesis / Q000235:genetics*; D009522:Newcastle disease virus / Q000201:enzymology / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D014765:Viral Vaccines
D000911:Antibodies, Monoclonal; D015707:HN Protein; D014765:Viral Vaccines