The adeno-associated virus rep gene suppresses herpes simplex virus-induced DNA amplification.
Herpes simplex virus (HSV) induces within the host cell genome DNA amplification which can be suppressed by coinfection with adeno-associated virus (AAV). To characterize the AAV functions mediating this effect, cloned AAV type 2 wild-type or mutant genomes were transfected into simian virus 40 (SV40)-transformed hamster cells together with the six HSV replication genes (encoding UL5, UL8, major DNA-binding protein, DNA polymerase, UL42, and UL52) which together are necessary and sufficient for the induction of SV40 DNA amplification (R. Heilbronn and H. zur Hausen, J. Virol. 63:3683-3692, 1989). The AAV rep gene was identified as being responsible for the complete inhibition of HSV-induced SV40 DNA amplification. Likewise, rep inhibited origin-dependent HSV replication. rep neither killed the transfected host cells nor interfered with gene expression from the cotransfected amplification genes. This points to a specific interference with HSV-induced DNA amplification.
Heilbronn
R
R
Deutsches Krebsforschungszentrum, Heidelberg, Federal Republic of Germany.
Bürkle
A
A
Stephan
S
S
zur Hausen
H
H
D002460:Cell Line; D002472:Cell Transformation, Viral; D003001:Cloning, Molecular; D004261:DNA Replication; D000229:Dependovirus / Q000235:genetics* / Q000502:physiology; D005784:Gene Amplification; D005814:Genes, Viral; D006367:HeLa Cells / Q000378:metabolism; D006378:Helper Viruses / Q000235:genetics; D006801:Humans; D009154:Mutation; D010957:Plasmids; D018139:Simplexvirus / Q000235:genetics*; D013489:Suppression, Genetic; D014162:Transfection; D014779:Virus Replication