An ESR study of pathologic red blood cell membranes (RBCM).
A comparative study of red blood cell membranes from normal subjects and beta-thalassemia and sickle cell anemia patients was performed by spin labeling at the lipidic and protein phase. The results show that the quantity of bound spin label is the same for sickle, thalassemic, and normal membranes. The data from 5-doxyl stearic acid suggest an increase in fluidity for the thalassemic membrane.
Perussi
J R
JR
Departamento de Química e Física Molecular, Instituto de Física e Química (USP), São Carlos, SP, Brasil.
Costa
F F
FF
Baffa
O
O
Zago
M A
MA
Tinto
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MH
Tabak
M
M
D000328:Adult; D000755:Anemia, Sickle Cell / Q000097:blood*; D002462:Cell Membrane / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D004913:Erythrocytes, Abnormal / Q000737:chemistry*; D006801:Humans; D008301:Maleimides / Q000032:analysis / Q000097:blood; D008565:Membrane Proteins / Q000032:analysis; D013057:Spectrum Analysis; D013113:Spin Labels; D013789:Thalassemia / Q000097:blood*
D008301:Maleimides; D008565:Membrane Proteins; D013113:Spin Labels; C043592:maleimide