Desensitization of Bacillus subtilis aspartokinase I to allosteric inhibition by meso-diaminopimelate allows aspartokinase I to function in amino acid biosynthesis during exponential growth.
Strains of Bacillus subtilis deficient in aspartokinases II and III are unable to grow in the absence of lysine, methionine, and threonine, although they have normal levels of aspartokinase I (J.J. Zhang, F.M. Hu, N.Y. Chen, and H. Paulus, J. Bacteriol. 172:701-708, 1990). Revertants with the ability to grow in the absence of lysine and methionine had an altered aspartokinase I, which was insensitive to feedback inhibition by meso-diaminopimelate. This suggests that inhibition by meso-diaminopimelate limits the ability of aspartokinase I to function in amino acid biosynthesis.
Zhang
J J
JJ
Department of Metabolic Regulation, Boston Biomedical Research Institute, Massachusetts 02114.
Paulus
H
H
D000494:Allosteric Regulation; D000599:Amino Acids, Diamino / Q000494:pharmacology*; D001222:Aspartate Kinase / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000302:isolation & purification; D001224:Aspartic Acid / Q000096:biosynthesis; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D003960:Diaminopimelic Acid / Q000494:pharmacology*; D007527:Isoenzymes / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000302:isolation & purification; D007700:Kinetics; D010770:Phosphotransferases / Q000037:antagonists & inhibitors*
D000599:Amino Acids, Diamino; D007527:Isoenzymes; D001224:Aspartic Acid; D003960:Diaminopimelic Acid; D010770:Phosphotransferases; D001222:Aspartate Kinase