title: Nucleotide sequence and analysis of the Vibrio alginolyticus sucrose uptake-encoding region.
abstract: The nucleotide sequence of the Vibrio alginolyticus sucrose uptake-encoding region was determined, and contained two genes, scrA and scrK. The scrA gene encodes an enzyme IISucrose (EIIScr) protein of the phosphoenolpyruvate dependent phosphotransferase system and the scrK gene encodes a fructokinase. The deduced amino acid (aa) sequence for the V. alginolyticus EIIScr protein was homologous with the EIIScr proteins from Streptococcus mutans, Salmonella typhimurium (pUR400 system) and Bacillus subtilis. The deduced aa sequence for the V. alginolyticus fructokinase was homologous with the Escherichia coli enzymes, 6-phosphofructokinase (isoenzyme 2) and ribokinase. Transposon phoA mutagenesis experiments indicated that the EIIScr protein was a membrane-bound protein with a region that extended into the periplasm.
authors.affiliation: Department of Microbiology, University of Cape Town, Rondebosch, South Africa.
authors.forename: G L
authors.initials: GL
authors.lastname: Blatch
authors.forename: R R
authors.initials: RR
authors.lastname: Scholle
authors.forename: D R
authors.initials: DR
authors.lastname: Woods
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics; D001483:Base Sequence; D004251:DNA Transposable Elements; D005631:Fructokinases / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics*; D015183:Restriction Mapping; D012486:Salmonella typhimurium / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013295:Streptococcus mutans / Q000235:genetics; D013395:Sucrose / Q000378:metabolism*; D014733:Vibrio / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D013395:Sucrose; D005631:Fructokinases; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C055701:phosphoenolpyruvate-sucrose phosphotransferase; C046173:fructokinase