Effect of long-term PUVA treatment of psoriasis on the collagen and elastin gene expression and growth of skin fibroblasts in vitro.
The proliferation rate, collagen metabolism and collagen and elastin messenger-RNA levels were studied in fibroblasts derived from patients who had received many courses of either systemic 8-methoxypsoralen or topical trioxsalen PUVA treatment. The proliferation rate of fibroblasts as measured by the incorporation of 3H-thymidine or by cellular division was decreased in those obtained from patients who had PUVA treatment as compared with controls. Collagen synthesis was slightly increased in the cells from PUVA-treated patients, but the relative collagen synthesis and the ratio between types I and III collagen were unchanged. The levels of collagen and elastin mRNAs were increased in fibroblasts derived from the PUVA-treated patients. No significant differences in histology or immunochemistry could be found in the biopsies taken from topical and systemic PUVA-treated patients.
Oikarinen
A
A
Department of Dermatology, University of Oulu, Finland.
Ala-Kokko
L
L
Tamminen
M
M
Karvonen
J
J
Reunala
T
T
Kallioinen
M
M
Hannuksela
M
M
D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D004549:Elastin / Q000235:genetics*; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D008938:Mitosis; D011701:PUVA Therapy; D011393:Procollagen-Proline Dioxygenase / Q000378:metabolism; D011565:Psoriasis / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis*; D012867:Skin / Q000473:pathology
D012333:RNA, Messenger; D003094:Collagen; D004549:Elastin; D011393:Procollagen-Proline Dioxygenase