Phytohemagglutinin-induced activity of cyclic AMP (cAMP) response elements from cytomegalovirus is reduced by cyclosporine and synergistically enhanced by cAMP.
The 19-base-pair enhancer repeat of the human cytomegalovirus immediate-early 1 gene mediates cyclic AMP- and phytohemagglutinin-induced expression in Jurkat T cells. Synergistic activity was observed in the presence of both drugs, suggesting a convergence of the protein kinase A and C pathways on this transcription element. In addition, the immunosuppressive drug cyclosporine strongly reduced the ability of the 19-base-pair repeat to activate gene expression in phytohemagglutinin-stimulated T cells.
Niller
H H
HH
Laboratory of Biochemistry and Metabolism, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.
Hennighausen
L
L
D001483:Base Sequence; D002460:Cell Line; D000242:Cyclic AMP / Q000494:pharmacology*; D003524:Cyclosporins / Q000494:pharmacology*; D003587:Cytomegalovirus / Q000187:drug effects / Q000235:genetics*; D004742:Enhancer Elements, Genetic / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects*; D005814:Genes, Viral / Q000187:drug effects*; D006801:Humans; D008969:Molecular Sequence Data; D010835:Phytohemagglutinins / Q000494:pharmacology*; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
D003524:Cyclosporins; D010835:Phytohemagglutinins; D000242:Cyclic AMP