A developmentally regulated and cAMP-repressible gene of Dictyostelium discoideum: cloning and expression of the gene encoding cyclic nucleotide phosphodiesterase inhibitor.
A 1.6-kb genomic fragment containing the coding region for the inhibitor (PDI) of cyclic nucleotide phosphodiesterase (PD) was isolated and sequenced. The genomic sequence includes 510 nucleotides (nt) of 5'-noncoding sequence and the full coding sequence, which contains two small introns. From the deduced amino acid (aa) sequence we predict a 26-kDa protein that, in agreement with previous data, contains approximately 15% Cys residues. The PDI possesses a hydrophobic leader sequence, five potential glycosylation sites, and three internal repeats. Northern-blot analysis showed a single transcript of 0.95 kb. The gene encoding PDI (pdi) was expressed early in development with little transcript remaining following aggregation. The appearance of pdi transcript was inhibited by cAMP, but when cAMP was removed the transcript appeared within 30 min. When cAMP was applied to cells containing pdi mRNA, the transcript disappeared with a half-life of less than 30 min.
Wu
L
L
Department of Anatomy and Cell Biology, College of Physicians and Surgeons of Columbia University, New York, NY 10032.
Franke
J
J
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004023:Dictyostelium / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D004794:Enzyme Repression; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005809:Genes, Regulator; D006023:Glycoproteins; D007438:Introns; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
D005656:Fungal Proteins; D006023:Glycoproteins; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases