title: 3'-Fluoro-3'-deoxy analogs of 2-5A 5'-monophosphate: binding to 2-5A-dependent endoribonuclease and susceptibility to (2'-5')phosphodiesterase degradation.
abstract: Analogs of 2-5A trimer 5'-monophosphate (2'-5')pA3,p5'A2'p5'A2'p5'A containing 9-(3-fluoro-3-deoxy-c-D-xylofuranosyl)adenine (AF) or 3'-fluoro-3'- deoxyadenosine (AF) at different positions of the chain have been synthesized. All of them were compared with (2'-5')pA3 and (2'-5')pA2 (3'dA) by (i) their ability to bind to 2-5A-dependent endoribonuclease(RNase L) of mouse L cells and of rabbit reticulocyte lysates and (ii) their susceptibility to the degradation by the (2'-5')phosphodiesterase activity. The results of this study suggest that the oligonucleotide conformation is important for its biochemical properties.
authors.affiliation: Institute of Bioorganic Chemistry, Byelorussian Academy of Sciences, Minsk, USSR.
authors.forename: E N
authors.initials: EN
authors.lastname: Kalinichenko
authors.forename: T L
authors.initials: TL
authors.lastname: Podkopaeva
authors.forename: M
authors.initials: M
authors.lastname: Kelve
authors.forename: M
authors.initials: M
authors.lastname: Saarma
authors.forename: I A
authors.initials: IA
authors.lastname: Mikhailopulo
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D004722:Endoribonucleases / Q000378:metabolism*; D051379:Mice; D009843:Oligoribonucleotides / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D011817:Rabbits
chemical_list: D000227:Adenine Nucleotides; D009843:Oligoribonucleotides; C023505:2',5'-oligoadenylate; D004722:Endoribonucleases; C031956:2-5A-dependent ribonuclease; D010727:Phosphoric Diester Hydrolases