title: Are there isoenzymes of cytochrome c oxidase in Paracoccus denitrificans?
abstract: We have used a gene replacement strategy to delete the previously isolated gene [(1987) EMBO J. 6, 2825-2833] for the cytochrome c oxidase subunit I from Paracoccus denitrificans. The resulting mutant was still able to synthesize active cytochrome c oxidase. This led us to look for another locus which could completely suppress the mutation. In this study we report the isolation of a second gene encoding subunit I. An open reading frame coding for cytochrome c 550 was found upstream from this gene. We suggest that there are isoenzymes of cytochrome c oxidase (cytochrome aa3) in this bacterium.
authors.affiliation: Department of Medical Chemistry, University of Helsinki, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Raitio
authors.forename: J M
authors.initials: JM
authors.lastname: Pispa
authors.forename: T
authors.initials: T
authors.lastname: Metso
authors.forename: M
authors.initials: M
authors.lastname: Saraste
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010231:Paracoccus denitrificans / Q000201:enzymology* / Q000235:genetics; D010957:Plasmids; D015183:Restriction Mapping
chemical_list: D004269:DNA, Bacterial; D007527:Isoenzymes; D003576:Electron Transport Complex IV