beta-Arrestin: a protein that regulates beta-adrenergic receptor function.
Homologous or agonist-specific desensitization of beta-adrenergic receptors is thought to be mediated by a specific kinase, the beta-adrenergic receptor kinase (beta ARK). However, recent data suggest that a cofactor is required for this kinase to inhibit receptor function. The complementary DNA for such a cofactor was cloned and found to encode a 418-amino acid protein homologous to the retinal protein arrestin. The protein, termed beta-arrestin, was expressed and partially purified. It inhibited the signaling function of beta ARK-phosphorylated beta-adrenergic receptors by more than 75 percent, but not that of rhodopsin. It is proposed that beta-arrestin in concert with beta ARK effects homologous desensitization of beta-adrenergic receptors.
Lohse
M J
MJ
Howard Hughes Medical Institute, Department of Medicine, Biochemistry and Cell Biology, Durham, NC 27710.
Benovic
J L
JL
Codina
J
J
Caron
M G
MG
Lefkowitz
R J
RJ
D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D019393:Arrestin; D015152:Blotting, Northern; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D017868:Cyclic AMP-Dependent Protein Kinases; D004247:DNA / Q000235:genetics; D005136:Eye Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D010766:Phosphorylation; D011494:Protein Kinases / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D011943:Receptors, Adrenergic, beta / Q000187:drug effects* / Q000502:physiology; D014162:Transfection; D051552:beta-Adrenergic Receptor Kinases
D000941:Antigens; D019393:Arrestin; D005136:Eye Proteins; D010726:Phosphodiesterase Inhibitors; D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta; D004247:DNA; D011494:Protein Kinases; D017868:Cyclic AMP-Dependent Protein Kinases; D051552:beta-Adrenergic Receptor Kinases