Control of the interferon-induced 68-kilodalton protein kinase by the HIV-1 tat gene product.
The tat-responsive region (TAR) of the human immunodeficiency virus-1 (HIV-1) exhibits a trans-inhibitory effect on translation in vitro by activating the interferon-induced 68-kilodalton protein kinase (p68 kinase). Productive infection by HIV-1 was shown to result in a significant decrease in the amount of cellular p68 kinase. The steady-state amount of p68 kinase was also reduced in interferon-treated HeLa cell lines stably expressing tat, as compared to the amount of the kinase in interferon-treated control HeLa cells. Thus, the potential translational inhibitory effects of the TAR RNA region mediated by activation of p68 kinase may be downregulated by tat during productive HIV-1 infection.
Roy
S
S
Department of Biochemistry, McGill University, Montreal, Canada.
Katze
M G
MG
Parkin
N T
NT
Edery
I
I
Hovanessian
A G
AG
Sonenberg
N
N
D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics; D015536:Down-Regulation; D004790:Enzyme Induction; D015971:Gene Expression Regulation, Enzymologic; D015696:Gene Products, tat / Q000502:physiology*; D005814:Genes, Viral; D015756:Genes, tat; D015497:HIV-1 / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D007163:Immunosorbent Techniques; D007370:Interferon Type I / Q000494:pharmacology*; D008970:Molecular Weight; D011494:Protein Kinases / Q000096:biosynthesis / Q000235:genetics*; D015534:Trans-Activators / Q000502:physiology*; D014162:Transfection; D054322:tat Gene Products, Human Immunodeficiency Virus
D015696:Gene Products, tat; D007370:Interferon Type I; D015534:Trans-Activators; D054322:tat Gene Products, Human Immunodeficiency Virus; D011494:Protein Kinases; D015088:2',5'-Oligoadenylate Synthetase