title: Use of gel filtration in the preparation of biological fluids for magnetic resonance spectroscopy.
abstract: Analysis of biological fluids by proton magnetic resonance spectroscopy is often complicated by dynamic range problems created from the large water resonance. Gel filtration chromatography is found to be a simple and nondestructive method for exchanging D2O for H2O and for removing low molecular weight molecules from both plasma and urine, significantly improving subsequent one- and two-dimensional MRS spectra.
authors.affiliation: Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710.
authors.forename: D W
authors.initials: DW
authors.lastname: Hoffman
authors.forename: R A
authors.initials: RA
authors.lastname: Venters
authors.forename: S F
authors.initials: SF
authors.lastname: Shedd
authors.forename: L D
authors.initials: LD
authors.lastname: Spicer
mesh_terms: D001834:Body Water / Q000032:analysis* / Q000502:physiology; D002850:Chromatography, Gel; D006801:Humans; D008055:Lipids / Q000032:analysis* / Q000097:blood; D009682:Magnetic Resonance Spectroscopy; D011522:Protons; D014556:Urine / Q000032:analysis*
chemical_list: D008055:Lipids; D011522:Protons