Isolation and characterization of protein kinase C from human placenta.
Protein kinase C (Ca2+ and phospholipid-dependent protein kinase) was detected in human placenta and was partially purified using DEAE cellulose chromatography and Ultrogel filtration. Diolein alone did not act on this enzyme but exerted a strong stimulatory action when associated with phosphatidylserine and Ca2+. Similar results were obtained with the phorbol myristate acetate. The kinetic constants for ATP, histone HI or Mg2+ and the apparent Ka for Ca2+ and phosphatidylserine were determined.
Tertrin-Clary
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C
Laboratoire des Hormones Polypeptidiques, CNRS, Gifsur-Yvette Cedex, France.
Chenut
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MC
de la Llosa
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P
D000255:Adenosine Triphosphate / Q000493:pharmacokinetics; D002118:Calcium / Q000494:pharmacology; D002147:Calmodulin / Q000494:pharmacology; D002848:Chromatography, DEAE-Cellulose; D000242:Cyclic AMP / Q000502:physiology; D004075:Diglycerides / Q000494:pharmacology; D005260:Female; D005374:Filtration; D006657:Histones / Q000493:pharmacokinetics; D006801:Humans; D008274:Magnesium / Q000493:pharmacokinetics; D010718:Phosphatidylserines / Q000494:pharmacology; D010920:Placenta / Q000032:analysis*; D011247:Pregnancy; D011493:Protein Kinase C / Q000302:isolation & purification*; D011494:Protein Kinases / Q000032:analysis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D002147:Calmodulin; D004075:Diglycerides; D006657:Histones; D010718:Phosphatidylserines; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D011494:Protein Kinases; D011493:Protein Kinase C; D008274:Magnesium; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium; C013965:diolein