title: The methanol-oxidizing system of Methylobacterium extorquens AM1 reconstituted with purified constituents.
abstract: The electron transport system (with cytochrome aa3) coupled to the oxidation of methanol in Methylobacterium extorquens AM1 (former Pseudomonas AM1) was reconstituted with highly purified constituents of the system. A mixture of 2.7 microM methanol dehydrogenase, 3.2 microM cytochrome cH, and 71 nM cytochrome c oxidase (= cytochrome aa3) consumed oxygen at a lower rate in the presence of methanol, while its activity was enhanced 3-fold by the addition of 1.4 microM cytochrome cL (74 mol of O2 consumed/mol of heme a of cytochrome c oxidase per min). Further addition of amicyanin to the above mixture did not affect the activity. Although ammonium ion greatly activated the activity of methanol dehydrogenase, the ion had little effect on the oxygen consumption activity of the above mixture. On the basis of the results obtained in the present study, an electron transport system is proposed for the oxidation of methanol in M. extorquens AM1.
authors.affiliation: Department of Life Science, Faculty of Science, Tokyo Institute of Technology.
authors.forename: K
authors.initials: K
authors.lastname: Mukai
authors.forename: Y
authors.initials: Y
authors.lastname: Fukumori
authors.forename: T
authors.initials: T
authors.lastname: Yamanaka
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000643:Ammonium Chloride / Q000494:pharmacology; D001426:Bacterial Proteins / Q000494:pharmacology; D003574:Cytochrome c Group / Q000378:metabolism*; D004579:Electron Transport; D003576:Electron Transport Complex IV / Q000378:metabolism*; D006088:Gram-Negative Aerobic Bacteria / Q000187:drug effects / Q000201:enzymology*; D000432:Methanol / Q000378:metabolism; D010084:Oxidation-Reduction; D010101:Oxygen Consumption
chemical_list: D001426:Bacterial Proteins; D003574:Cytochrome c Group; C017079:mauC protein, Methylobacterium extorquens; D000643:Ammonium Chloride; D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor); D003576:Electron Transport Complex IV; D000432:Methanol