Two systems for the automated analysis of drugs in biological fluids using high-performance liquid chromatography.
This paper describes two fully automated assays. One for zaprinast, a cGMP specific phosphodiesterase inhibitor, which uses the Gilson-Advanced Automated Sample Processor combination, and the other for an H+/K+ ATPase inhibitor and its sulphone metabolite, which uses direct injection. Both assays were developed to support pharmacokinetic studies at therapeutic doses in small animals as well as in man. Plasma or serum (20-200 microliters) is placed directly into an autosampler and all subsequent manipulations are performed mechanically.
Doyle
E
E
Department of Drug Analysis, Smith Kline and French Research Ltd., Frythe, Welwyn, Hertfordshire, U.K.
McDowall
R D
RD
Murkitt
G S
GS
Picot
V S
VS
Rogers
S J
SJ
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000037:antagonists & inhibitors*; D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors*; D000818:Animals; D001562:Benzimidazoles / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D017506:H(+)-K(+)-Exchanging ATPase; D006801:Humans; D009853:Omeprazole / Q000031:analogs & derivatives; D000077402:Pantoprazole; D011688:Purinones / Q000097:blood*; D011725:Pyridines / Q000097:blood*; D013454:Sulfoxides / Q000097:blood*
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D001562:Benzimidazoles; D011688:Purinones; D011725:Pyridines; D013454:Sulfoxides; C064731:5-difluoromethoxy-2-((3,4 dimethoxy-2-pyridyl)methylsulfonyl)-1H-benzimidazole; D000077402:Pantoprazole; D015106:3',5'-Cyclic-GMP Phosphodiesterases; D000251:Adenosine Triphosphatases; D017506:H(+)-K(+)-Exchanging ATPase; C011145:zaprinast; D009853:Omeprazole