title: Electron microscopy and image analysis of two-dimensional crystals and single molecules of alcohol oxidase from Hansenula polymorpha.
abstract: The octameric protein alcohol oxidase from the yeast Hansenula polymorpha was studied by electron microscopy and image analysis. Two-dimensional crystals were formed by applying the protein, in a phosphate buffer containing poly(ethylene glycol) and EDTA, to a carbon-coated formvar film which had been glow-discharged in pentylamine at least several hours earlier. The crystals show p4 symmetry and have a unit cell of 12.5 X 12.5 nm2, containing one molecule. Image analysis of the crystals and of single molecules yielded two different views. From these it can be deduced that the subunits have an elongated shape and form two layers of four, stacked face to face. A tentative model of the structure is presented.
authors.affiliation: Biochemical Laboratory, Groningen University, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Vonck
authors.forename: E F
authors.initials: EF
authors.lastname: van Bruggen
mesh_terms: D000429:Alcohol Oxidoreductases; D003198:Computer Simulation; D003461:Crystallography; D005656:Fungal Proteins / Q000648:ultrastructure; D007091:Image Processing, Computer-Assisted; D008854:Microscopy, Electron; D010843:Pichia / Q000201:enzymology*; D011487:Protein Conformation; D004718:Saccharomycetales / Q000201:enzymology*
chemical_list: D005656:Fungal Proteins; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase