title: Insertion of the fusion gene from Newcastle disease virus into a non-essential region in the terminal repeats of fowlpox virus and demonstration of protective immunity induced by the recombinant.
abstract: In this paper we report on the identification of non-essential genes in the terminal repeats of the avipox-virus fowlpox virus and the use of these as insertion sites in a vector system. Foreign genes inserted into these sites are shown to be present in two copies in the resultant recombinant virus. To test the potential use of this vector as a live vaccine the fusion gene of Newcastle disease virus has been inserted into a vaccine strain of fowlpox virus and inoculated into chickens. The experiments demonstrate the ability of the recombinant to protect chickens against challenge by a virulent strain of Newcastle disease virus and to elicit the formation of an anti-fusion protein antibody.
authors.affiliation: Institute for Animal Health, Houghton Laboratory, Huntingdon, Cambridgeshire, U.K.
authors.forename: M E
authors.initials: ME
authors.lastname: Boursnell
authors.forename: P F
authors.initials: PF
authors.lastname: Green
authors.forename: J I
authors.initials: JI
authors.lastname: Campbell
authors.forename: A
authors.initials: A
authors.lastname: Deuter
authors.forename: R W
authors.initials: RW
authors.lastname: Peters
authors.forename: F M
authors.initials: FM
authors.lastname: Tomley
authors.forename: A C
authors.initials: AC
authors.lastname: Samson
authors.forename: P
authors.initials: P
authors.lastname: Chambers
authors.forename: P T
authors.initials: PT
authors.lastname: Emmerson
authors.forename: M M
authors.initials: MM
authors.lastname: Binns
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002645:Chickens; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004797:Enzyme-Linked Immunosorbent Assay; D005587:Fowlpox virus / Q000235:genetics* / Q000276:immunology; D005814:Genes, Viral; D005822:Genetic Vectors; D006385:Hemagglutination Inhibition Tests; D007109:Immunity; D009522:Newcastle disease virus / Q000235:genetics* / Q000276:immunology; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping
chemical_list: D004251:DNA Transposable Elements