title: A rapid screen for the detection of specific DNA sequences in plants.
abstract: We have developed a simple and quick method ("wick blot") for detecting the presence of specific DNA sequences in plants, using radiolabeled DNA probes. The method requires only small amounts of tissue, about 15-25 mg. More than a hundred samples per day can be easily extracted and blotted. It works well on various species and tissues, including leaves, embryos, and callus. The method is ideally suited for screening large numbers of putative transformants, especially populations that have not been screened by prior selection.
authors.affiliation: Calgene, Inc., Davis, CA 95616.
authors.forename: J M
authors.initials: JM
authors.lastname: Irvine
authors.forename: J V
authors.initials: JV
authors.lastname: Oakes
authors.forename: C K
authors.initials: CK
authors.lastname: Shewmaker
authors.forename: A
authors.initials: A
authors.lastname: Crossway
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D015342:DNA Probes; D015336:Molecular Probe Techniques; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000235:genetics*; D010947:Plants, Toxic; D014026:Nicotiana / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D015342:DNA Probes; D004247:DNA