title: Human liver fibroblast capacity for synthesizing interstitial collagenase in vitro.
abstract: si e s------------------------ABSTRACT------------------------- P4 initiates specifically the degradation of interstitial collagen types I-III. This enzyme is thus directly involved in the remodeling of the connective matrix. Fibroblasts are considered as the major source of interstitial collagenase in many tissues. However previous studies shown that liver fibroblasts did not spontaneously produce the enzyme. We have thus measured the steady-state levels of mRNA for interstitial procollagenase and quantified interstitial collagenase activity in cultured human liver fibroblasts, in presence or not of interleukin-1 or tumor necrosis factor. We demonstrate that human liver fibroblasts have the capacity for producing interstitial collagenase and that this production is regulated at a transcriptional step. We suggest that the liver fibroblast could represent a key cell for therapeutic strategies of fibrosis reversal.
authors.affiliation: CNRS URA 602, Institut Pasteur, Lyon, France.
authors.forename: H
authors.initials: H
authors.lastname: Emonard
authors.forename: A
authors.initials: A
authors.lastname: Guillouzo
authors.forename: C M
authors.initials: CM
authors.lastname: Lapiere
authors.forename: J A
authors.initials: JA
authors.lastname: Grimaud
mesh_terms: D017364:Collagenases; D004792:Enzyme Precursors / Q000378:metabolism; D005109:Extracellular Matrix / Q000201:enzymology; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D003012:Microbial Collagenase / Q000096:biosynthesis* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D004792:Enzyme Precursors; D007375:Interleukin-1; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D017364:Collagenases; C022440:procollagenase; D003012:Microbial Collagenase