Cloning of the cellulase gene from Penicillium funiculosum and its expression in Escherichia coli.
A gene of Penicillium funiculosum encoding an endoglucanase was cloned and expressed in Escherichia coli using the lacZ promoter of vector pUC 18. The gene product hydrolyzed carboxymethyl cellulose and showed strong cross reactivity with P. funiculosum anticellulases.
Sahasrabudhe
N A
NA
Division of Biochemical Sciences, National Chemical Laboratory, Poona, India.
Ranjekar
P K
PK
D002480:Cellulase / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D005800:Genes, Fungal; D005822:Genetic Vectors; D010407:Penicillium / Q000201:enzymology / Q000235:genetics*
D002480:Cellulase