Cloned murine T lymphocytes synthesize a molecule with the biological characteristics of nitric oxide.
Recently, activated neutrophils and macrophages have been shown to synthesize nitric oxide (NO) exclusively from L-arginine. We searched for the presence of this pathway in murine T cell clones. Using a platelet aggregation bioassay sensitive to NO, we demonstrate that IL2-stimulated CTLL and HT2 cells inhibit platelet aggregation, whereas unstimulated lymphocytes do not. This action can be inhibited by the specific NO synthase competitor NG-mono-methyl arginine, and only the L form of arginine or its analogue L-homoarginine are capable of providing substrate for NO synthesis.
Kirk
S J
SJ
Department of Surgery, Sinai Hospital of Baltimore, MD.
Regan
M C
MC
Barbul
A
A
D000818:Animals; D001120:Arginine / Q000494:pharmacology; D002460:Cell Line; D002999:Clone Cells / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism; D010974:Platelet Aggregation / Q000187:drug effects; D051381:Rats; D013601:T-Lymphocytes / Q000378:metabolism*; D013917:Thrombin / Q000494:pharmacology
D009573:Nitrites; D009569:Nitric Oxide; D001120:Arginine; D013917:Thrombin; D006152:Cyclic GMP