Detection of transforming growth factor alpha in human urine and plasma.
A sensitive enzyme-linked immunosorbent assay (ELISA) system for human transforming growth factor alpha (TGF alpha) was developed in combination with polyclonal and monoclonal antibodies. Employing this assay system, we detected TGF alpha like activity in normal human plasma as well as in cancer patients' urine and plasma. These TGF alpha were analyzed by chromatography, immunoreactivity, and EGF-TGF alpha receptor binding assay and found to be identical to authentic human TGF alpha. The presence of TGF alpha circulating in normal adult plasma suggests a new role of TGF alpha in the human body.
Katoh
M
M
Laboratory for Cell Biology, Hoechst Japan Limited, Saitama.
Inagaki
H
H
Kurosawa-Ohsawa
K
K
Katsuura
M
M
Tanaka
S
S
D015415:Biomarkers / Q000097:blood* / Q000652:urine; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000652:urine; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003110:Colonic Neoplasms / Q000097:blood / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood*; D013274:Stomach Neoplasms / Q000097:blood / Q000652:urine; D015291:Transforming Growth Factors / Q000097:blood* / Q000302:isolation & purification / Q000652:urine
D015415:Biomarkers; D015291:Transforming Growth Factors