Interleukin-2 inhibits the replication of human herpesvirus-6 in mature thymocytes.
Human herpesvirus-6 (HHV-6) is a recently identified T lymphotropic virus. We have examined the ability of HHV-6 to replicate in mature and immature human thymocytes. Infection of both cell populations revealed that only mitogen-activated mature thymocytes could support efficient virus replication. Because interleukin-2 (IL-2) plays a central role in T cell activation we investigated its effect on HHV-6 replication. Unexpectedly, addition of recombinant IL-2 at concentrations-exceeding 10 U/ml strongly inhibited the virus-induced cytopathic effect. Electron microscopic examinations and immunofluorescence assays revealed a threefold reduction in the fraction of infected cells, and almost total absence of extracellular virions in the IL-2-treated cultures. It will therefore be of interest to determine whether the IL-2-mediated inhibitory effect plays some role in the establishment of HHV-6 latency in the human host.
Roffman
E
E
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases/Twinbrook, National Institutes of Health, Rockville, Maryland 20852.
Frenkel
N
N
D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D005455:Fluorescent Antibody Technique; D015654:Herpesvirus 6, Human / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D008854:Microscopy, Electron; D011994:Recombinant Proteins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000382:microbiology* / Q000648:ultrastructure; D014771:Virion / Q000648:ultrastructure; D014779:Virus Replication
D007376:Interleukin-2; D011994:Recombinant Proteins