Regulated expression of Epstein-Barr virus nuclear antigen 3-encoding gene carried on stable episomal vectors in human cells.
Recently a small number of Epstein-Barr viral (EBV) genes, characteristically expressed in latently infected, growth-transformed B-lymphocytes, have been cloned and several have been transiently expressed by DNA transfection. Here we demonstrate production of stable human cell lines containing episomal EBV vectors and expressing EBV nuclear antigen 3 from the adenovirus major late promoter or the mouse metallothionein promoter, which retains metal-regulation in the episomal state. This system has proved useful in an analysis of the role of these and other EBV genes implicated in immortalization and/or oncogenic transformation of human cells.
James
M R
MR
Institut de Recherches Scientifiques sur le Cancer, Villejuif, France.
Sarasin
A R
AR
Perricaudet
M
M
Joab
I
I
D000956:Antigens, Viral / Q000235:genetics*; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003001:Cloning, Molecular; D019309:Epstein-Barr Virus Nuclear Antigens; D015870:Gene Expression; D005814:Genes, Viral; D005822:Genetic Vectors; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007700:Kinetics; D015183:Restriction Mapping; D014162:Transfection; D015678:Viral Structural Proteins / Q000235:genetics*
D000956:Antigens, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D015678:Viral Structural Proteins; D015500:Chloramphenicol O-Acetyltransferase