DNA gyrase gyrA mutations in ciprofloxacin-resistant strains of Staphylococcus aureus: close similarity with quinolone resistance mutations in Escherichia coli.
The gyrA genes isolated from three ciprofloxacin-resistant clinical isolates of Staphylococcus aureus carried codon 84 (serine----leucine) and/or codon 85 (serine----proline) mutations that were absent in pretreatment susceptible strains. These substitutions occur in a region of the gyrase A protein wherein directly analogous mutations of serine 83----leucine and alanine 84----proline in Escherichia coli confer quinolone resistance. Thus, DNA gyrase A subunit mutations are implicated in resistance to ciprofloxacin in S. aureus.
Sreedharan
S
S
Department of Cellular and Molecular Sciences, St. George's Hospital Medical School, University of London, United Kingdom.
Oram
M
M
Jensen
B
B
Peterson
L R
LR
Fisher
L M
LM
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002939:Ciprofloxacin / Q000494:pharmacology*; D004250:DNA Topoisomerases, Type II / Q000235:genetics*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics*
D002939:Ciprofloxacin; D004250:DNA Topoisomerases, Type II