title: Purification of polymeric phospholipase Cs from human platelets.
abstract: Two types of cytosolic phospholipase C specific for phosphoinositides were purified from human platelets. The molecular masses of the purified enzymes were 440 and 290 kDa. These enzymes were concluded to be respectively a trimer and a dimer of homologous 146 kDa polypeptides. The 146 kDa polypeptide may be an immunologically novel isozyme among the 140-150 kDa PLC isozymes. Both enzymes hydrolyzed phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate in a Ca2(+)-dependent manner.
authors.affiliation: Research Institute for Food Science, Kyoto University.
authors.forename: T
authors.initials: T
authors.lastname: Moriyama
authors.forename: H
authors.initials: H
authors.lastname: Narita
authors.forename: M
authors.initials: M
authors.lastname: Oki
authors.forename: T
authors.initials: T
authors.lastname: Matsuura
authors.forename: M
authors.initials: M
authors.lastname: Kito
mesh_terms: D001792:Blood Platelets / Q000187:drug effects / Q000201:enzymology*; D002118:Calcium / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007527:Isoenzymes / Q000737:chemistry / Q000302:isolation & purification*; D008970:Molecular Weight; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols / Q000378:metabolism; D012680:Sensitivity and Specificity; D013379:Substrate Specificity; D010738:Type C Phospholipases / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D007527:Isoenzymes; D019269:Phosphatidylinositol 4,5-Diphosphate; D010716:Phosphatidylinositols; D010738:Type C Phospholipases; D002118:Calcium