Mg.ATP primes superoxide-generating responses in electropermeabilized neutrophils.
The present study utilizes an electropermeabilized cell system to determine the effect of Mg.ATP on neutrophil superoxide (O2-)-generating responses stimulated by suboptimal concentrations of fMLP, GTP gamma S and PMA. Permeabilization in the presence of exogenously added Mg.ATP was neither sufficient to initiate O2- release nor necessary for stimulated O2- production. However, the inclusion of Mg.ATP in the permeabilization medium primed the O2(-)-generating responses mediated by suboptimal concentrations of these stimuli. The site of action of Mg.ATP is intracellular. Moreover, the fact that Mg.ATP primes responses stimulated by fMLP, GTP gamma S and PMA suggests that the modulatory effect is at the level of protein kinase C.
Hartfield
P J
PJ
Department of Cell Biology, Neurobiology and Anatomy, Ohio State University, Columbus 43210.
Robinson
J M
JM
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D002463:Cell Membrane Permeability; D004560:Electricity; D004790:Enzyme Induction / Q000187:drug effects; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D019255:NADPH Oxidases; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013873:Thionucleotides / Q000494:pharmacology
D013873:Thionucleotides; D013481:Superoxides; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D009240:N-Formylmethionine Leucyl-Phenylalanine; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases; D013755:Tetradecanoylphorbol Acetate