Method to identify genomic targets of DNA binding proteins.
We have devised a cyclical immunoprecipitation protocol that can be used to identify and clone a specific DNA sequence that is recognized by a DNA binding protein, even if that sequence is present in only one copy in the genome of a mammal. As an example, we have used this procedure to purify mouse genomic sequences to which the simian virus 40 tumor (T) antigen binds.
Sompayrac
L
L
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309-0347.
Danna
K J
KJ
D000260:Adenoviruses, Human / Q000235:genetics / Q000276:immunology; D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D001665:Binding Sites; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D015698:Genomic Library; D006801:Humans; D051379:Mice; D010957:Plasmids; D013539:Simian virus 40 / Q000235:genetics / Q000276:immunology; D013211:Staphylococcus aureus / Q000235:genetics
D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D004247:DNA