The 20-pS chloride channel of the human airway epithelium.
The single-channel inside-out patch clamp technique was used to characterize chloride channels in the apical membranes of human airway epithelial cells maintained in primary culture. Patches were obtained from single isolated cells or from cells at the edges of confluent groups. The channel seen most often, in 24% of all patches, had a conductance of approximately 20 pS and had a linear current-voltage relationship in symmetric chloride solutions. The anion selectivity sequence for the channel was NO3- greater than Cl- greater than HCO3-, and it was impermeable to gluconate ions, indicating that the channel diameter lies between 4.7 and 5.5 A. Current through the channel saturated at high chloride concentrations, and the relationship between channel current and chloride concentration could be approximated by the Michaelis-Menten equation. Analysis of the channel's anion permeability and its current vs. concentration relationship indicates that it can be described by the one-ion channel theory, with a relatively weak binding site inside the channel. Histograms of channel open and closed durations were constructed using the log binning technique and could be well fitted by triple exponential distributions, suggesting that the channel has at least three open and three closed states.
Duszyk
M
M
Department of Physiology, University of Alberta, Edmonton, Canada.
French
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D002151:Calorimetry; D018118:Chloride Channels; D002712:Chlorides / Q000502:physiology*; D004553:Electric Conductivity; D004848:Epithelium / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008433:Mathematics; D008564:Membrane Potentials; D008565:Membrane Proteins / Q000502:physiology*; D008954:Models, Biological; D009994:Osmolar Concentration; D014420:Turbinates / Q000502:physiology*
D018118:Chloride Channels; D002712:Chlorides; D008565:Membrane Proteins