title: Dietary canola oil: effect on the accumulation of eicosapentaenoic acid in the alkenylacyl fraction of human platelet ethanolamine phosphoglyceride.
abstract: Volunteers consumed a mixed-fat diet for 6 d (Pre-exp) and then either a canola-oil-based diet (CAN) containing linolenic acid (18:3n-3) or a sunflower-oil-based diet (SUN) rich in linoleic acid (18:2n-6) for 18 d, followed by the alternative diet in a crossover design. Platelet phospholipids were analyzed for changes in fatty acid composition. Eicosapentaenoic acid (EPA) (20:5n-3) was significantly higher in alkenylacyl ethanolamine phosphoglyceride (PPE) and in total phosphatidylcholine (PC) after CAN compared with SUN and Pre-exp. The 22:5n-3 was increased in PPE after CAN above concentrations found after both SUN and Pre-exp. Lower concentrations of 20:4n-6 and 22:4n-6 were observed with CAN in PC and lower concentrations of 22:4n-6 in PPE. These results indicate that the consumption of canola oil moderately increases EPA concentrations and alters the concentrations of other n-6 and n-3 fatty acids in human platelet phospholipids.
authors.affiliation: Department of Nutritional Sciences, University of Guelph, Ontario, Canada.
authors.forename: B J
authors.initials: BJ
authors.lastname: Weaver
authors.forename: E J
authors.initials: EJ
authors.lastname: Corner
authors.forename: V M
authors.initials: VM
authors.lastname: Bruce
authors.forename: B E
authors.initials: BE
authors.lastname: McDonald
authors.forename: B J
authors.initials: BJ
authors.lastname: Holub
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000378:metabolism*; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D015118:Eicosapentaenoic Acid / Q000097:blood*; D005229:Fatty Acids, Monounsaturated / Q000494:pharmacology*; D006801:Humans; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology; D008042:Linolenic Acids / Q000494:pharmacology; D008297:Male; D010713:Phosphatidylcholines / Q000097:blood; D010714:Phosphatidylethanolamines / Q000097:blood*; D010743:Phospholipids / Q000097:blood; D010938:Plant Oils / Q000494:pharmacology; D016032:Randomized Controlled Trials as Topic; D000074262:Rapeseed Oil; D000074242:Sunflower Oil
chemical_list: D004042:Dietary Fats, Unsaturated; D005229:Fatty Acids, Monounsaturated; D008041:Linoleic Acids; D008042:Linolenic Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D010938:Plant Oils; D000074262:Rapeseed Oil; D000074242:Sunflower Oil; D019787:Linoleic Acid; D015118:Eicosapentaenoic Acid