title: Development of a new Leydig cell population after the destruction of existing Leydig cells by ethane dimethane sulphonate in rats: an autoradiographic study.
abstract: The formation of new Leydig cells in adult male rats was studied after the complete destruction of the original population by ethane dimethane sulphonate (EDS). Following administration of EDS, proliferating interstitial cells were labelled in a pulse-chase experiment by way of three [3H]thymidine injections on days 2, 3 and 4 after EDS administration. Some of the newly formed Leydig cells found 14 days after EDS administration were labelled with [3H]thymidine, indicating that these Leydig cells were derived from precursor cells, most likely mesenchymal cells, that had incorporated [3H]thymidine at days 2, 3 or 4 after EDS administration. At 21 days after EDS administration, the total number of Leydig cells (labelled plus unlabelled) had increased 7- to 16-fold compared with the number of cells that were present 14 days after EDS had been administered. In a second series of experiments, [3H]thymidine was given 2 h before the rats were killed (short-term labelling experiment). In this experiment it was shown that the proliferative activity of the mesenchymal cells, which are presumed to be the precursors of the Leydig cells, after a considerable increase at day 2 after EDS administration, had returned to the control level at day 7. However, the total number of mesenchymal cells (labelled plus unlabelled) remained increased from 2 to 49 days after EDS administration. This indicated that the majority of the new Leydig cells which were formed from day 14 onwards probably did not derive from differentiating mesenchymal cells.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Cell Biology, Medical School, State University of Utrecht, The Netherlands.
authors.forename: K J
authors.initials: KJ
authors.lastname: Teerds
authors.forename: D G
authors.initials: DG
authors.lastname: de Rooij
authors.forename: F F
authors.initials: FF
authors.lastname: Rommerts
authors.forename: C J
authors.initials: CJ
authors.lastname: Wensing
mesh_terms: D000818:Animals; D001345:Autoradiography; D002455:Cell Division; D007985:Leydig Cells / Q000166:cytology* / Q000187:drug effects; D008297:Male; D008648:Mesoderm / Q000166:cytology; D008698:Mesylates / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D008698:Mesylates; C002994:ethylene dimethanesulfonate