Single-step labeling of DNA using restriction endonucleases and T4 polynucleotide kinase.
DNA restriction fragments can be end-labeled in a combined digestion and labeling reaction with restriction endonuclease plus T4 polynucleotide kinase, omitting a dephosphorylation step. Both pBR322 and phage lambda DNA digests are efficiently labeled in one step, one tube and one incubation.
Oommen
A
A
Dept. of Biochemistry, University of Kansas, Lawrence 66045-2106.
Ferrandis
I
I
Wang
M J
MJ
D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D007553:Isotope Labeling / Q000379:methods*; D010770:Phosphotransferases; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D013604:T-Phages / Q000235:genetics
D004279:DNA, Viral; D010770:Phosphotransferases; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D004262:DNA Restriction Enzymes