Cell type-specific induction of the major immediate early enhancer of human cytomegalovirus by cyclic AMP.
The major immediate early enhancer of human cytomegalovirus (HCMV) is known to exert a strong constitutive transcription stimulation in a broad spectrum of cells. This basal activity can be augmented considerably by elevated levels of intracellular cAMP in a cell type-specific manner. Cyclic AMP induction was observed in several lymphoid cell lines and in HeLa cells. One of the functionally important enhancer sequence modules, the 19 bp repeat element, mediates this effect as a cAMP-responsive element (CRE). It acts more efficiently than the corresponding sequence from the human chorionic gonadotropin gene. It is suggested that protein kinase C is involved in the pathway which leads to the activation of CRE-containing genes in lymphoid cells. Gel retardation assays indicated that similar, but not identical complexes are formed between nuclear protein extracts and the CREs of HCMV and the gonadotropin gene.
Stamminger
T
T
Institut für Klinische und Molekulare Virologie, Universität Erlangen-Nürnberg, F.R.G.
Fickenscher
H
H
Fleckenstein
B
B
D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D003587:Cytomegalovirus / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D015967:Gene Expression Regulation, Viral; D006367:HeLa Cells; D006801:Humans; D017874:Immediate-Early Proteins; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D012091:Repetitive Sequences, Nucleic Acid; D019311:Staurosporine; D014162:Transfection
D000470:Alkaloids; D000956:Antigens, Viral; D017874:Immediate-Early Proteins; D009841:Oligonucleotides; C044582:immediate-early proteins, cytomegalovirus; D005576:Colforsin; D000242:Cyclic AMP; D011493:Protein Kinase C; D019311:Staurosporine