title: Sandwich enzyme immunoassay and latex agglutination test for herpes simplex virus keratitis.
abstract: We determined the in vitro sensitivities and the diagnostic efficacies of a commercially available latex agglutination test and an enzyme-linked immunosorbent assay using a rabbit model of herpes simplex virus type 1 dendritic keratitis. In contrast to the latex agglutination test, the HERPCHEK enzyme-linked immunosorbent assay offers an extremely sensitive and reliable means of laboratory diagnosis of experimental herpes simplex virus keratitis and is more rapid than viral isolation in tissue culture.
authors.affiliation: Department of Ophthalmology, Washington University School of Medicine, St. Louis, Missouri 63110.
authors.forename: S F
authors.initials: SF
authors.lastname: Lee
authors.forename: J S
authors.initials: JS
authors.lastname: Pepose
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007635:Keratitis, Dendritic / Q000175:diagnosis*; D007841:Latex Fixation Tests; D011817:Rabbits; D018139:Simplexvirus / Q000302:isolation & purification