Incidence of growth of syngeneic oral squamous cell carcinoma in C57B1 bg/bg beige mice.
The incidence of tumor growth in C57B1 beige mice and the cytotoxic response generated toward a syngeneic oral squamous cell carcinoma cell line (A5) were investigated. Viable A5 tumor cells were inoculated into the left hind limb of the mice and the tumor volume was determined weekly. Cytotoxicity in mice was generated in vivo immunization with viable A5 tumor cells. Splenic lymphocytes were restimulated in a mixed lymphocyte-tumor cell culture to enhance their cytotoxicity, which was determined via a 51Chromium release microcytotoxicity assay. The result shows a higher incidence of tumor growth in beige mice as well as less cytotoxicity than in their littermate control. These studies suggest that higher incidence of tumor growth in beige mice may be due to the impairment in cytotoxic T cell activity rather than a pure NK cell deficit.
Wong
D Y
DY
Department of Dentistry, Veterans General Hospital-Taipei, National Yang Ming Medical College, R.O.C.
Feinberg
S E
SE
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000473:pathology*; D003602:Cytotoxicity, Immunologic; D005260:Female; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D008817:Mice, Mutant Strains; D009062:Mouth Neoplasms / Q000276:immunology / Q000473:pathology*; D009368:Neoplasm Transplantation; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014185:Transplantation, Isogeneic