Dexamethasone increases the activity but not the amount of choline-phosphate cytidylyltransferase in fetal rat lung.
The activity of choline-phosphate cytidylyltransferase is increased by glucocorticoids in late gestation fetal lung in association with increased phosphatidylcholine biosynthesis. Previous indirect data had suggested that the stimulatory effect of the hormone was due to activation of existing enzyme rather than synthesis of new cytidylyltransferase protein. Using a rabbit antibody raised against purified rat liver choline-phosphate cytidylyltransferase, we have now quantitated the amount of the enzyme in fetal rat lung explants cultured with and without dexamethasone. Our results show that the hormone increased the activity of the enzyme but not the amount of cytidylyltransferase protein. Thus the stimulatory effect of dexamethasone on cytidylyltransferase is due to activation of existing enzyme rather than induction of enzyme synthesis.
Rooney
S A
SA
Department of Pediatrics, Yale University School of Medicine, New Haven, CT.
Smart
D A
DA
Weinhold
P A
PA
Feldman
D A
DA
D000818:Animals; D015153:Blotting, Western; D019916:Choline-Phosphate Cytidylyltransferase; D003907:Dexamethasone / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D005865:Gestational Age; D007163:Immunosorbent Techniques; D008168:Lung / Q000187:drug effects / Q000196:embryology* / Q000201:enzymology; D009713:Nucleotidyltransferases / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains
D010713:Phosphatidylcholines; D003907:Dexamethasone; D009713:Nucleotidyltransferases; D019916:Choline-Phosphate Cytidylyltransferase