Herpes simplex virus 1 infection upregulates stress protein expression in cultured retinal neurons.
The production of a 57K stress protein (StrP) after herpes simplex virus type 1 (HSV-1) infection was examined in cultured neonatal rat retinal cells. StrP expression in individual cells was identified using a monoclonal antibody, TI56. Indirect immunofluorescence of uninfected retinal cultures showed that approximately 40% of cells expressed neurofilament (NF+) and 5% expressed a low level of StrP. Following HSV infection the proportion of NF+ cells decreased while the proportion of StrP positive cells became greater and the intensity of staining increased. The number of cells labelled with a polyclonal anti-HSV antibody increased with time after infection. Retinal neurons in culture can be infected with HSV, after which StrP expression is significantly upregulated.
Kennedy
P G
PG
Department of Neurology, Tennent Institute of Ophthalmology, Glasgow.
Wakakura
M
M
Foulds
W S
WS
Clements
G B
GB
D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral / Q000502:physiology; D005455:Fluorescent Antibody Technique; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D007381:Intermediate Filament Proteins / Q000096:biosynthesis; D007635:Keratitis, Dendritic / Q000382:microbiology*; D008856:Microscopy, Fluorescence; D016900:Neurofilament Proteins; D009474:Neurons / Q000382:microbiology; D051381:Rats; D012173:Retinitis / Q000382:microbiology*; D018139:Simplexvirus / Q000472:pathogenicity*
D000911:Antibodies, Monoclonal; D006360:Heat-Shock Proteins; D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins