Morphine induces an intracellular alkalinization in bovine aortic endothelial cells (BAECs).
The resting intracellular pH (pHi) of BAECs, by using BCECF fluorescence, at 37 degrees C in Na+ Hanks' was 7.22 +/- 0.03. Cells which had been acid-loaded recovered from the intracellular acidification in Na+ Hanks' in a [Na+]o-dependent and amiloride-sensitive manner. Recovery from acidification had an apparent Km for Na+ of 40 +/- 10 mM and Ki for amiloride of 26 +/- 4 microM. Morphine (50 microM, 20 min, 37 degrees C) increased the pHi to 7.55 +/- 0.05. Naloxone (50 microM) given 5 min before morphine (50 microM) blocked this effect, indicating that this was an opiate receptor-mediated phenomenon. To determine if morphine activated the Na+/H+ exchanger, pHi was monitored in Na+-free Hanks', acidic Na+ Hanks' or amiloride-containing Na+ Hanks'. The alkalinization produced by morphine was not observed under all these circumstances. These data suggest morphine activates the Na+/H+ exchanger via opiate receptors.
Kiang
J G
JG
Department of Physiology, Armed Forces Radiobiology Research Institute, Bethesda, Maryland 20814-5145.
Colden-Stanfield
M
M
D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007424:Intracellular Fluid / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D009020:Morphine / Q000494:pharmacology*; D011957:Receptors, Opioid / Q000187:drug effects / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers
D002352:Carrier Proteins; D011957:Receptors, Opioid; D017923:Sodium-Hydrogen Exchangers; D009020:Morphine