title: Atomic-resolution structure of the cellulose synthase regulator cyclic diguanylic acid.
abstract: Cyclic diguanylic acid acts as a regulator for cellulose synthase activity in the bacterium Acetobacter xylinum. We report the x-ray crystal structure of the regulator at atomic resolution. The structure contains two independent molecules that adopt almost identical conformations. The two molecules form self-intercalated units that are stacked on each other. Two different G.G base-pairing modes occur between the stacks. The more stable one has two or possibly three hydrogen bonds between two guanines and is related to the type of hydrogen bonding that is believed to exist between G-rich strands at the ends of chromosomes.
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
authors.forename: M
authors.initials: M
authors.lastname: Egli
authors.forename: R V
authors.initials: RV
authors.lastname: Gessner
authors.forename: L D
authors.initials: LD
authors.lastname: Williams
authors.forename: G J
authors.initials: GJ
authors.lastname: Quigley
authors.forename: G A
authors.initials: GA
authors.lastname: van der Marel
authors.forename: J H
authors.initials: JH
authors.lastname: van Boom
authors.forename: A
authors.initials: A
authors.lastname: Rich
authors.forename: C A
authors.initials: CA
authors.lastname: Frederick
mesh_terms: D000091:Acetobacter / Q000201:enzymology; D001482:Base Composition; D006152:Cyclic GMP / Q000031:analogs & derivatives*; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors; D006860:Hydrogen Bonding; D008958:Models, Molecular; D008968:Molecular Conformation; D015394:Molecular Structure; D014961:X-Ray Diffraction
chemical_list: C062025:bis(3',5')-cyclic diguanylic acid; D005964:Glucosyltransferases; C063760:cellulose synthase (GDP-forming); D006152:Cyclic GMP