Rapid isolation of genomic DNA from animal tissues.
A simple technique for the isolation of very high molecular weight genomic DNA from animal tissues and cells is described. The method involves rapid isolation of nuclei and their embedding in agarose beads followed by extraction of lipids and proteins with SDS. The protocol does not require proteolytic digestion and the whole procedure can be completed in 1 day. The isolated DNA is digestible by restriction enzymes and free of ligase inhibitors.
Konat
G
G
Department of Neurology, Medical University of South Carolina, Charleston.
Gantt
G
G
Laszkiewicz
I
I
Hogan
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EL
D000818:Animals; D002457:Cell Extracts / Q000032:analysis; D002458:Cell Fractionation / Q000379:methods*; D002467:Cell Nucleus / Q000737:chemistry; D002498:Centrifugation; D004247:DNA / Q000032:analysis / Q000187:drug effects / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D012685:Sepharose
D002457:Cell Extracts; D004247:DNA; D012685:Sepharose; D004262:DNA Restriction Enzymes