A DNA gyrase-binding site at the center of the bacteriophage Mu genome is required for efficient replicative transposition.
We have discovered a centrally located site that is required for efficient replication of bacteriophage Mu DNA and identified it as a strong DNA gyrase-binding site. Incubation of Mu DNA with gyrase and enoxacin revealed a cleavage site 18.1 kilobases from the left end of the 37.2-kilobase genome. Two observations indicate a role for the site in Mu replication: mutants of Mu, able to grow on an Escherichia coli gyrB host that does not allow growth of wild-type Mu, were found to possess single-base changes resulting in more efficient gyrase binding and cleavage at the site. Introduction of a 147-base-pair deletion that eliminated the site from a prophage inhibited the onset of Mu replication for greater than 1 hr after induction.
Pato
M L
ML
Department of Microbiology and Immunology, University of Colorado Health Sciences Center, Denver 80262.
Howe
M M
MM
Higgins
N P
NP
D010583:Bacteriophage mu / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D004261:DNA Replication; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D004251:DNA Transposable Elements; D004278:DNA, Superhelical / Q000502:physiology; D004279:DNA, Viral / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D015183:Restriction Mapping; D014779:Virus Replication
D004251:DNA Transposable Elements; D004278:DNA, Superhelical; D004279:DNA, Viral; D004250:DNA Topoisomerases, Type II