title: Elimination of mycoplasmal plate agglutination cross-reactions in sera from chickens inoculated with infectious bursal disease viruses.
abstract: Sera from chickens inoculated with various challenge infectious bursal disease viruses or infectious bursal disease vaccines were found to cross-react in the Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) serum plate agglutination (SPA) tests. Two-fold dilutions of these cross-reacting sera with phosphate-buffered saline before retesting eliminated all non-specific agglutination in the MG and MS SPA tests. Cross-reactions were observed in the SPA test using sera from chickens inoculated with either MG or MS. Dilutions of these sera 1:2 had little effect on the number of these cross-reactions. At 1:4 serum dilutions, however, the number of cross-reactions between MG and MS was reduced. At 1:8 dilution of test sera, cross-reactions between MG and MS were further reduced. Some reduction in specific MG and MS SPA reactions, however, also occurred at the 1:8 dilution of sera with some of the plate antigen.
authors.affiliation: Department of Animal and Poultry Sciences, University of Arkansas, Fayetteville 72701.
authors.forename: T
authors.initials: T
authors.lastname: Ross
authors.forename: M
authors.initials: M
authors.lastname: Slavik
authors.forename: G
authors.initials: G
authors.lastname: Bayyari
authors.forename: J
authors.initials: J
authors.lastname: Skeeles
mesh_terms: D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D002645:Chickens; D003429:Cross Reactions; D005189:False Positive Reactions; D007106:Immune Sera / Q000276:immunology; D007243:Infectious bursal disease virus / Q000276:immunology*; D009174:Mycoplasma / Q000276:immunology*; D011201:Poultry Diseases / Q000276:immunology*; D012088:Reoviridae Infections / Q000276:immunology / Q000662:veterinary*; D013047:Specific Pathogen-Free Organisms; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000914:Antibodies, Viral; D007106:Immune Sera; D014765:Viral Vaccines