Detection of staphylococcal enterotoxin B. A comparative study of ELISA and ELIFA systems.
The enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunofiltration assay (ELIFA) sandwich methods have been compared for their ability to detect staphylococcal enterotoxin B (SEB) in biological samples. Using the avidin-biotin amplification system in the ELISA, high sensitivity was observed and staphylococcal protein A interference was completely prevented. In contrast, ELIFA was less sensitive than ELISA, but very easy to perform, fast and simple to read routinely.
Dupont
H
H
Centre d'Etudes du Bouchet, Vert-le-Petit, France.
Therasse
J
J
Pinon
J M
JM
Binder
P
P
D000818:Animals; D000911:Antibodies, Monoclonal; D001710:Biotin; D004768:Enterotoxins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis / Q000379:methods; D006735:Horseradish Peroxidase; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D015203:Reproducibility of Results
D000911:Antibodies, Monoclonal; D004768:Enterotoxins; C031818:enterotoxin B, staphylococcal; D001710:Biotin; D006735:Horseradish Peroxidase