RNA analysis and isolation of cDNAs derived from the human cytomegalovirus immediate-early region at 0.24 map units.
We have examined transcription from the human cytomegalovirus immediate-early genes that are located at approximately 0.24 map units of the viral genome. Upon infection of permissive cells, nonoverlapping transcripts of 1.65 and 1.7 kb were abundant 8 h after infection and at lower levels at later times of infection or in the presence of cycloheximide. A transcript of approximately 3.4 kb that spanned the entire region was less abundant than the smaller RNAs at 8 h after infection, but was not decreased in abundance with cycloheximide treatment. These RNA species appear to correspond to the immediate-early transcripts from this region. Polyadenylated RNA was isolated from human cytomegalovirus infected cells 8 h after infection and used to synthesize directly a cDNA library. cDNAs corresponding to the abundant 1.65- and 1.7-kb transcripts of this region were isolated and characterized.
Tenney
D J
DJ
E.I. du Pont de Nemours & Co., Inc., Central Research and Development, Wilmington, Del. 19880-0328.
Colberg-Poley
A M
AM
D015152:Blotting, Northern; D015139:Blotting, Southern; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D005814:Genes, Viral / Q000235:genetics*; D006801:Humans; D012367:RNA, Viral / Q000096:biosynthesis* / Q000235:genetics; D015183:Restriction Mapping; D014158:Transcription, Genetic / Q000235:genetics
D004279:DNA, Viral; D012367:RNA, Viral