Multiple primer pairs polymerase chain reaction for the detection of human papillomavirus types.
A polymerase chain reaction (PCR) based on the use of multiple primers enabling the simultaneous detection of HPV-6b, -11, -16 and -18 in a single tube reaction was developed and validated on cervico-vaginal specimens, including tissues embedded in paraffin. This PCR setting proved to be specific and sensitive, allowing the detection of as few as 200 viral particles per specimen.
Anceschi
M M
MM
Istituto di Ginecologia ed Ostetricia, Policlinico Monteluce, Perugia, Italy.
Falcinelli
C
C
Pieretti
M
M
Cosmi
E V
EV
D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000382:microbiology; D002584:Cervix Uteri / Q000382:microbiology; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005260:Female; D005784:Gene Amplification; D006801:Humans; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D013698:Templates, Genetic; D002583:Uterine Cervical Neoplasms / Q000382:microbiology; D014621:Vagina / Q000382:microbiology
D015344:DNA Probes, HPV; D004279:DNA, Viral