Differentiation of vaccine and field strains of bovine herpesvirus type 1 by restriction endonuclease analysis.
Bovine herpesvirus type 1 (BHV-1) DNA molecules obtained from a limited number of infected cells were cleaved with a variety of restriction endonucleases. By use of selected DNA fragments in Bgl 1, Pst 1, Pvu 1 and Pvu 11 restriction patterns, one reference, two vaccine and three wild-type strains of BHV-1 were distinguished from one another. The simplified DNA fingerprinting method described here should be most useful, not only to control the genetic stability of BHV-1 vaccines during production, but also to differentiate the vaccine strains from other isolates in clinical cases.
Hamelin
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C
Centre de recherche en virologie, Institut Armand-Frappier, Laval-des-Rapides, Québec, Canada.
Jacques
C
C
Assaf
R
R
D000818:Animals; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis*; D007242:Herpesvirus 1, Bovine / Q000145:classification* / Q000276:immunology; D015183:Restriction Mapping; D013045:Species Specificity; D014765:Viral Vaccines
D004279:DNA, Viral; D014765:Viral Vaccines; D004262:DNA Restriction Enzymes