title: Wnt-3, a gene activated by proviral insertion in mouse mammary tumors, is homologous to int-1/Wnt-1 and is normally expressed in mouse embryos and adult brain.
abstract: We have isolated a common insertion site, Wnt-3, for proviruses of the mouse mammary tumor virus (MMTV). Of mammary tumors induced by the GR variant of MMTV, 5% contains a provirus at Wnt-3, which is located on mouse chromosome 11. The gene is transcribed into a 3.8-kilobase (kb) mRNA in tumors with nearby proviral insertions but not in tumors with proviruses at other loci or in most adult tissues. Normal expression of Wnt-3 is detected in mouse embryos (with a peak around day 12 of gestation) and at low levels in adult brain. The transcriptional unit of the Wnt-3 gene spans approximately 55 kb, with a first intron of 36 kb. The deduced amino acid sequence of the Wnt-3 protein is 47% identical to the int-1/Wnt-1 gene product.
authors.affiliation: Division of Molecular Biology, Netherlands Cancer Institute, Amsterdam.
authors.forename: H
authors.initials: H
authors.lastname: Roelink
authors.forename: E
authors.initials: E
authors.lastname: Wagenaar
authors.forename: S
authors.initials: S
authors.lastname: Lopes da Silva
authors.forename: R
authors.initials: R
authors.lastname: Nusse
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000382:microbiology*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D004622:Embryo, Mammalian; D004625:Embryo, Nonmammalian; D015870:Gene Expression; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D008325:Mammary Neoplasms, Experimental / Q000382:microbiology*; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics*; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D011533:Proviruses / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D051153:Wnt Proteins; D060508:Wnt3 Protein
chemical_list: D004279:DNA, Viral; D011506:Proteins; D051153:Wnt Proteins; D060508:Wnt3 Protein; C555508:Wnt3 protein, mouse