Effect of Mycobacterium leprae's phenolic glycolipid-I on interferon-gamma augmentation of monocyte oxidative responses.
Peripheral blood monocytes were pretreated with phenolic glycolipid-I (PGL-I), dimycocerosyl phthiocerol (DIM), or mycoside A, then cultured in the presence or absence of interferon-gamma (IFN-gamma). Their oxidative responses to Mycobacterium leprae, phorbol myristate acetate (PMA), and opsonized zymosan were evaluated. In response to M. leprae, monocytes pretreated with PGL-I released less O2- than nonlipid-treated control cells. The IFN-gamma augmentation of oxidative responses was suppressed only when in PGL-I-pretreated monocytes and only when the stimulus was M. leprae. This suggests that PGL-I, by affecting the IFN-gamma enhancement of phagocytic cell oxidative responses, aids further the intracellular survival of M. leprae.
Vachula
M
M
Department of Medicine, University of Illinois, Chicago.
Holzer
T J
TJ
Kizlaitis
L
L
Andersen
B R
BR
D000942:Antigens, Bacterial / Q000276:immunology*; D002478:Cells, Cultured; D006017:Glycolipids / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*; D009166:Mycobacterium leprae / Q000276:immunology*; D010084:Oxidation-Reduction; D013481:Superoxides / Q000378:metabolism
D000942:Antigens, Bacterial; D006017:Glycolipids; C045169:phenolic glycolipid I, Mycobacterium leprae; D013481:Superoxides; D007371:Interferon-gamma