Multiple sequence elements in the U3 region of the leukemogenic murine retrovirus SL3-2 contribute to cell-dependent gene expression.
Determination of the U3 sequence of the leukemogenic murine retrovirus SL3-2 revealed close relationships to SL3-3, Akv, and Gross passage A viruses. The SL3-2 and Akv regions showed wide differences in their relative transcriptional activity in four cell lines as determined by U3-driven transient expression assays. The U3 regions of SL3-2 and SL3-3 gave rise to similar but not identical levels of expression. Deletion mapping of the SL3-2 U3 region points to several determinants of expression of different relative importance in the cell lines tested.
Dai
H Y
HY
Department of Molecular Biology and Plant Physiology, University of Aarhus, Denmark.
Etzerodt
M
M
Baekgaard
A J
AJ
Lovmand
S
S
Jørgensen
P
P
Kjeldgaard
N O
NO
Pedersen
F S
FS
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D009052:Leukemia Virus, Murine / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D004279:DNA, Viral