A one-step enzyme immunoassay for human manganese superoxide dismutase with monoclonal antibodies.
A one-step enzyme immunoassay for the determination of manganese superoxide dismutase in serum has been developed with two kinds of monoclonal antibodies. Proposed method had high sensitivity (assay range, 0.4-200 ng/ml), good recovery (recovery percentage, 102.9-106.2%) and reproducibility (intraassay, C.V. = 1.87-3.66%; interassay, C.V. = 3.03-10.4%). From these results, it is possible to apply this method to routine clinical analysis and biochemical research with various purposes.
Adachi
T
T
Department of Pharmaceutics, Gifu Pharmaceutical University, Japan.
Hirano
K
K
Hayashi
K
K
Muto
Y
Y
Okuno
F
F
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D006801:Humans; D006825:Hybridomas; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D013482:Superoxide Dismutase / Q000032:analysis*
D000911:Antibodies, Monoclonal; D013482:Superoxide Dismutase