High- and low-density lipoproteins enhance infection of Trypanosoma cruzi in vitro.
Trypanosoma cruzi exhibits a developmentally regulated neuraminidase activity that is inhibited by high-density lipoprotein (HDL). We report here that the infection of culture cells by T. cruzi trypomastigotes is enhanced by HDL in a dose-dependent manner. The enhanced infection is prevented by Vibrio cholerae neuraminidase, an enzyme whose activity is not inhibited by HDL, suggesting that sialic acid is involved in T. cruzi-host interaction. Similar enhancement of infection is also produced by low-density lipoprotein (LDL), which inhibits T. cruzi neuraminidase as well as HDL. Further evidence that the enhancement is due to lipoproteins is provided by the fact that infection of host cells in lipoprotein-deficient medium is less than in normal medium; it can be restored to the higher level by the addition of HDL, LDL or both to the lipoprotein-deficient medium. In view of these results, we propose that HDL and LDL regulate T. cruzi infection in mammalian hosts by inhibiting the parasite neuraminidase activity.
Prioli
R P
RP
New England Medical Center Hospitals, Dept. of Geographic Medicine and Infectious Diseases, Boston, MA 02111.
Rosenberg
I
I
Pereira
M E
ME
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D006790:Host-Parasite Interactions; D006801:Humans; D008075:Lipoproteins, HDL / Q000502:physiology*; D008077:Lipoproteins, LDL / Q000502:physiology*; D009439:Neuraminidase / Q000037:antagonists & inhibitors; D014349:Trypanosoma cruzi / Q000472:pathogenicity*
D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D009439:Neuraminidase