Cytochrome P-455 nm complex formation in the metabolism of phenylalkylamines. XI. Peroxygenase versus monooxygenase function of cytochrome P-450 in rat liver microsomes.
Cytochrome P-455 nm complex formation in phenobarbital induced rat liver microsomes was investigated using both an NADPH/O2-dependent monooxygenase system and a peroxygenase/peroxidase system where hydrogen peroxide was substituted for NADPH. The substrates tested were the enantiomers of four 1-alkyl-substituted 2-phenylethanamines (unbranched 1-alkyl substituents, comprising one to four carbons), S(+)- and R(-)-N-hydroxyamphetamine and racemic mixtures of N-hydroxy-1-phenyl-2-butanamine and N-hydroxy-3-methyl-1-phenyl-2-butanamine. During NADPH/O2-dependent metabolism the amines showed a positive correlation between extent of complex formation and lipophilicity; furthermore the S(+)-isomers gave rise to larger amounts of complex than the corresponding R(-)-analogues. With the hydroxylamines the ability to form complexes was greater than with any of the amines but no definite difference was seen among the hydroxylamines. In the peroxygenase system the hydroxylamines still gave larger amounts of complex than the amines but the differences seen within the homologous series of chiral amines when using the monooxygenase system were no longer observed. Although the quantitative trends in complex formation seen in the monooxygenase system were non-existent when H2O2 was substituted for NADPH, mere qualitative rules still seemed to apply; substrates which failed to give the complex during NADPH-dependent metabolism (2-phenylethanamine, phentermine, N-hydroxyphentermine and phenylacetone oxime) were inactive also in the peroxygenase system. The results substantiate the notion that the monooxygenase and peroxygenase reaction mechanisms of cyt. P-450 follow similar but not identical pathways.
Jönsson
K H
KH
Department of Organic Pharmaceutical Chemistry, Uppsala University, Sweden.
Lindeke
B
B
D015078:2-Hydroxyphenethylamine / Q000378:metabolism; D000588:Amines / Q000378:metabolism*; D000662:Amphetamines / Q000378:metabolism*; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000502:physiology*; D003580:Cytochromes / Q000378:metabolism*; D005609:Free Radicals; D006864:Hydrogenase / Q000378:metabolism*; D006878:Hydroxides; D017665:Hydroxyl Radical; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D009249:NADP / Q000378:metabolism; D010105:Oxygenases / Q000502:physiology*; D010627:Phenethylamines / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013237:Stereoisomerism; D013379:Substrate Specificity
D000588:Amines; D000662:Amphetamines; D003580:Cytochromes; D005609:Free Radicals; D006878:Hydroxides; D010627:Phenethylamines; D017665:Hydroxyl Radical; D009249:NADP; C029251:cytochrome P455; D015078:2-Hydroxyphenethylamine; D003577:Cytochrome P-450 Enzyme System; D006864:Hydrogenase; D010105:Oxygenases