title: Inhibited enzyme electrodes. Part 1: Theoretical model.
abstract: A theoretical model is developed for an electrochemical sensor for toxic substances which works by measuring the inhibition of the enzyme activity. The enzyme is assumed to follow Michaelis-Menten kinetics and the diffusion kinetic equation describing the concentration profile of the enzyme's substrate in the electrolyte layer between the electrode and the membrane covering the electrode is solved. A complete set of analytical solutions is found which corresponds to a number of different rate limiting processes. The set of solutions is described in a case diagram. The use of cytochrome oxidase in particular is discussed.
authors.affiliation: Department of Chemistry, Imperial College, London, UK.
authors.forename: W J
authors.initials: WJ
authors.lastname: Albery
authors.forename: A E
authors.initials: AE
authors.lastname: Cass
authors.forename: Z X
authors.initials: ZX
authors.lastname: Shu
mesh_terms: D015374:Biosensing Techniques; D004058:Diffusion; D003576:Electron Transport Complex IV / Q000037:antagonists & inhibitors; D007700:Kinetics; D008962:Models, Theoretical; D014118:Toxins, Biological / Q000032:analysis
chemical_list: D014118:Toxins, Biological; D003576:Electron Transport Complex IV