title: Binding and regulation of cellular functions by monoclonal antibodies against human tumor necrosis factor receptors.
abstract: The present study was undertaken to further characterize the interaction of monoclonal antibodies (mAbs) against tumor necrosis factor (TNF) receptors with different targets, and to assess their ability to influence TNF effects on U937 and human endothelial cell (HEC) functions. Actions of recombinant TNF-alpha on U937 and HEC were effectively inhibited by Htr-5 and Utr-1, and to a greater extent by a combination of both mAbs. These observations indicate that TNF interaction with antigenically different components of membrane receptors (p55 and p75) represents a crucial step in transduction of signals for TNF toxicity against U937 and TNF activation of HEC functions.
authors.affiliation: Institute of Cancer Research, University of Trondheim, Norway.
authors.forename: M R
authors.initials: MR
authors.lastname: Shalaby
authors.forename: A
authors.initials: A
authors.lastname: Sundan
authors.forename: H
authors.initials: H
authors.lastname: Loetscher
authors.forename: M
authors.initials: M
authors.lastname: Brockhaus
authors.forename: W
authors.initials: W
authors.lastname: Lesslauer
authors.forename: T
authors.initials: T
authors.lastname: Espevik
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology / Q000502:physiology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure*; D006801:Humans; D007951:Leukemia, Myeloid / Q000378:metabolism / Q000473:pathology; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D018124:Receptors, Tumor Necrosis Factor; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism / Q000633:toxicity
chemical_list: D000911:Antibodies, Monoclonal; D011956:Receptors, Cell Surface; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha