title: Difference in effects of interferon-alpha and interferon-gamma on the induction of differentiation of retinoic acid-treated acute myeloid leukemia cells in primary culture.
abstract: We studied differentiation inducing effects of retinoic acid (RA), 1 alpha, 25-dihydroxyvitamin D3 (D3) and interferons (IFNs), alone and in combination, on fresh myeloid leukemic cells from 8 patients. RA not only induced the differentiation of leukemic cells in 5/8 cases, but potentiated differentiation by IFNs either in granulocytic or monocytic pathways. In particular, interferon-alpha enhanced granulocytic differentiation and interferon-gamma induced mono-macrophage differentiation of promyelocytic leukemic cells in the presence of RA. Differentiation induced by D3, alone or in combination with IFNs, was limited in all cases. RA plus IFNs might be an effective combination for differentiation therapy for some types of myeloid leukemia.
authors.affiliation: Department of Hematology, Showa University School of Medicine, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nakamaki
authors.forename: A
authors.initials: A
authors.lastname: Sakashita
authors.forename: K
authors.initials: K
authors.lastname: Hino
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: S
authors.initials: S
authors.lastname: Tomoyasu
authors.forename: N
authors.initials: N
authors.lastname: Tsuruoka
authors.forename: Y
authors.initials: Y
authors.lastname: Honma
authors.forename: M
authors.initials: M
authors.lastname: Hozumi
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D002762:Cholecalciferol / Q000494:pharmacology; D004950:Esterases / Q000032:analysis; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D009113:Muramidase / Q000032:analysis; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D007370:Interferon Type I; D002762:Cholecalciferol; D009580:Nitroblue Tetrazolium; D014212:Tretinoin; D007371:Interferon-gamma; D004950:Esterases; D009113:Muramidase