title: Neural transplantation using temperature-sensitive immortalized neural cells: a preliminary report.
abstract: Current approaches to neural transplantation--adrenal medullary and fetal brain--suffer from limitations in tissue availability, cellular uniformity, and general applicability. The system described here uses temperature-sensitive immortalized clonal neural cells to address these limitations. Cerebellar cells, immortalized with a temperature-sensitive tumor virus, are transformed at 34 degrees C, but differentiate at 38 degrees C. The cells were genetically engineered to express beta-galactosidase, providing a tag to study their fate in the host. The cells were then successfully transplanted to the cerebellum of adult rats.
authors.affiliation: University of California, San Francisco.
authors.forename: D E
authors.initials: DE
authors.lastname: Bredesen
authors.forename: K
authors.initials: K
authors.lastname: Hisanaga
authors.forename: F R
authors.initials: FR
authors.lastname: Sharp
mesh_terms: D000818:Animals; D001358:Avian Sarcoma Viruses; D002460:Cell Line; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000637:transplantation*; D046508:Culture Techniques / Q000379:methods*; D006085:Graft Survival; D051381:Rats