The use of the polymerase chain reaction for the detection of Simian immunodeficiency virus in experimentally infected macaques.
A rapid, non-radioactive assay for the detection of proviral Simian immunodeficiency virus (SIV) in tissue-culture cells is described. The assay is based on the co-amplification of the SIV env and gag genes by the polymerase chain reaction (PCR). When the gag PCR product is blotted onto a nylon membrane and hybridised to a radioactive oligonucleotide probe, the assay can also be used to detect the SIV gag gene in DNA isolated directly from experimentally infected cynomolgus macaque lymphocytes. This provides a valuable assay for the presence of proviral SIV during animal trials of AIDS vaccines and chemotherapeutics.
Kitchin
P A
PA
WHO AIDS Collaborating Centre, National Institute for Biological Standards and Control, South Mimms, Potters Bar, U.K.
Almond
N
N
Szotyori
Z
Z
Fromholc
C E
CE
McAlpine
L
L
Silvera
P
P
Stott
E J
EJ
Cranage
M
M
Baskerville
A
A
Schild
G
G
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005784:Gene Amplification; D015752:Genes, env; D015750:Genes, gag; D008214:Lymphocytes / Q000382:microbiology; D008252:Macaca fascicularis; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics / Q000302:isolation & purification; D012192:Retroviridae Infections / Q000382:microbiology*; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000302:isolation & purification*
D004279:DNA, Viral