title: Buccal mucosa fibroblasts and periodontal ligament cells perturbed by tensile stimuli in vitro.
abstract: Human buccal mucosa fibroblasts and periodontal ligament cells grown in tissue culture were subjected to tensile forces approximating those used for orthodontic bodily tooth movement. The cells were synchronized into pre S phase and positively tested for response to nonmechanical physical stimuli. Two-dimensional gel analysis and immunohistochemical analysis of the three cytoskeletal components showed a lack of response. Similar negative results were found when the cells were perturbed in the presence of substance P. We hypothesize that perhaps these cells respond more readily to injury, a secondary effect of the forces of tooth movement, than to tensile forces.
authors.affiliation: University of Connecticut Health Center, School of Dental Medicine, Farmington 06032.
authors.forename: L A
authors.initials: LA
authors.lastname: Norton
authors.forename: K L
authors.initials: KL
authors.lastname: Andersen
authors.forename: B
authors.initials: B
authors.lastname: Melsen
authors.forename: D A
authors.initials: DA
authors.lastname: Bindslev
authors.forename: J E
authors.initials: JE
authors.lastname: Celis
mesh_terms: D002462:Cell Membrane / Q000032:analysis / Q000502:physiology; D002467:Cell Nucleus / Q000032:analysis / Q000502:physiology; D002478:Cells, Cultured; D046508:Culture Techniques; D003584:Cytological Techniques; D003599:Cytoskeleton / Q000032:analysis / Q000502:physiology; D005347:Fibroblasts / Q000032:analysis / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000032:analysis*; D006801:Humans; D009061:Mouth Mucosa / Q000032:analysis / Q000166:cytology*; D010513:Periodontal Ligament / Q000032:analysis / Q000166:cytology*; D010812:Physical Stimulation; D013718:Tensile Strength; D014087:Tooth Movement Techniques
chemical_list: D006360:Heat-Shock Proteins