title: The gene family encoding eggshell proteins of Schistosoma japonicum.
abstract: The four closely related genes encoding eggshell proteins in the human parasite Schistosoma japonicum are described. A cDNA and a genomic DNA library were constructed and members of the eggshell protein gene family isolated. The four genes in this family do not contain introns, and differ in organization and nucleotide sequence from the related set of genes in Schistosoma mansoni and Schistosoma haematobium. The coding sequences of two of the S. japonicum genes and their flanking regions were determined. Transcription start sites for these genes were shown by primer extension analysis to occur 47 and 50 nucleotides in front of the start codon. A female-specific component in nuclear extracts binds to a DNA fragment containing conserved sequences upstream of the transcription start sites. The deduced protein sequences of 207 and 212 amino acids are composed of 50% glycine with continuous glycine regions as long as 11 residues. In vitro translations of male and female RNAs revealed female-specific translation products, the sizes of which were consistent with the eggshell proteins.
authors.affiliation: Department of Biochemistry, University of Iowa, Iowa City.
authors.forename: K J
authors.initials: KJ
authors.lastname: Henkle
authors.forename: G A
authors.initials: GA
authors.lastname: Cook
authors.forename: L A
authors.initials: LA
authors.lastname: Foster
authors.forename: D M
authors.initials: DM
authors.lastname: Engman
authors.forename: L A
authors.initials: LA
authors.lastname: Bobek
authors.forename: G D
authors.initials: GD
authors.lastname: Cain
authors.forename: J E
authors.initials: JE
authors.lastname: Donelson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003062:Codon; D004247:DNA / Q000737:chemistry; D004527:Egg Proteins / Q000096:biosynthesis / Q000235:genetics*; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D015698:Genomic Library; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D011401:Promoter Regions, Genetic; D012549:Schistosoma japonicum / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D003062:Codon; D004527:Egg Proteins; D004247:DNA