Oxidative burst in human Epstein-Barr virus-transformed B-cell lines triggered by immobilized specific antigen.
Normal human B lymphocytes and Epstein-Barr virus-transformed B-cell lines can produce reactive oxygen species such as superoxide if treated with phorbol myristate acetate (PMA) or with the surface immunoglobulin cross-linking agents protein A and anti-immunoglobulin. Here, we investigated under which conditions specific antigen, the natural ligand of surface immunoglobulin, can stimulate an oxidative burst in monoclonal Epstein-Barr virus-transformed B-cell lines producing antibodies of known specificities. After a short lag time of 1-2 min, exposure to the specific antigen stimulated a prolonged oxidative burst (tmax 30-90 min), as measured by Lucigenin-enhanced, superoxide dismutase-inhibitable chemiluminescence, in the corresponding line only. The effect was induced in each line if the specific antigen was immobilized to a solid support. Except in one line in which antigen also stimulated an oxidative burst if presented at relatively high density on a soluble carrier, soluble antigen did not induce B-cell oxidase activation. This suggests that normal, non-transformed B lymphocytes also require interaction with relatively dense deposits of specific antigen for activation of their oxidase.
Maly
F E
FE
Institute of Clinical Immunology, Inselspital Bern, Switzerland.
Urwyler
A
A
Tengler
R
R
Steinitz
M
M
Golding
B
B
de Weck
A L
AL
D000166:Acridines; D000941:Antigens / Q000276:immunology; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D002461:Cell Line, Transformed; D004854:Herpesvirus 4, Human; D006801:Humans; D008163:Luminescent Measurements; D010088:Oxidoreductases / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D013205:Staphylococcal Protein A / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000166:Acridines; D000941:Antigens; D013205:Staphylococcal Protein A; C033472:10,10'-dimethyl-9,9'-biacridinium; D010088:Oxidoreductases; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen