The novel inositol lipid phosphatidylinositol 3,4-bisphosphate is produced by human blood platelets upon thrombin stimulation.
Radioactive PtdIns(3)P was detected in human platelets incubated with [32P]Pi, but remained unaffected by thrombin treatment. In contrast, [32P]PtdIns(3,4)P2 was absent from resting platelets, but was produced by thrombin-activated platelets in a dose- and time-dependent manner. [32P]PtdInsP3 was never found under these conditions. These changes are similar to those elicited in other cells by platelet-derived growth factor or the oncogene product pp60c-src.
Sultan
C
C
INSERM Unité 326, Hôpital Purpan, Toulouse, France.
Breton
M
M
Mauco
G
G
Grondin
P
P
Plantavid
M
M
Chap
H
H
D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008055:Lipids / Q000096:biosynthesis / Q000097:blood; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols / Q000097:blood*; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D013268:Stimulation, Chemical; D013917:Thrombin / Q000494:pharmacology*; D013997:Time Factors
D008055:Lipids; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; D010761:Phosphorus Radioisotopes; C060980:phosphatidylinositol 3,4-diphosphate; D013917:Thrombin