title: Effect of obstructive jaundice on renal Na-K ATPase activity and ATP contents, their response to E. coli or endotoxin and effect of 1-6 fructose diphosphate.
abstract: A two-week bile duct ligation (BDL) in Sprague-Dawley (SD) rats raised the serum billirubin level and decreased the mean arterial blood pressure and renal cortical ATP contents compared with those in sham-operated (SO) rats (3.6 +/- 1.15 mg% vs 0.54 +/- 0.36, P less than 0.001; 69 +/- 24 mmHg vs 86 +/- 21, P less than 0.05; 3.72 +/- 0.86 x 10(-10) mol/mg tissue vs 7.27 +/- 0.18, P less than 0.05). No difference could be found in the medullary ATP contents (8.42 +/- 2.20 vs 8.70 +/- 2.80, P = NS). In SO rats, injection of endotoxin (0.7 mg/kg BW) and E. coli (3.1 x 10(5) bacteria/100 g BW) reduced cortical ATP content to 1.86 +/- 0.97 and 1.30 +/- 0.47 (P less than 0.001), and medullar ATP to 1.33 +/- 0.31 and 2.12 +/- 0.46 (P less than 0.001) respectively. In BDL rats, the same treatment led to further decrease in cortical ATP to 1.25 +/- 0.40 and 0.62 +/- 0.20, medullary ATP to 0.97 +/- 0.41 and 1.64 +/- 0.83 (P less than 0.001). Basal Na-K ATPase activity in BDL is the same compared with that in SO both in the cortex (2.85 +/- 2.2 mumol/mgpr/h vs 2.19 +/- 0.75; P = NS) and medulla (2.79 +/- 1.83 vs 3.05 +/- 1.38; P = NS).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Division of Nephrology, Huashan Hospital, Shanghai Medical University.
authors.forename: Y
authors.initials: Y
authors.lastname: Gu
authors.forename: W G
authors.initials: WG
authors.lastname: Wang
authors.forename: Y M
authors.initials: YM
authors.lastname: Gu
authors.forename: J B
authors.initials: JB
authors.lastname: Gu
authors.forename: S Y
authors.initials: SY
authors.lastname: Lin
mesh_terms: D058186:Acute Kidney Injury / Q000517:prevention & control; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001651:Cholestasis, Extrahepatic / Q000378:metabolism*; D003135:Common Bile Duct; D004731:Endotoxins; D004926:Escherichia coli; D005635:Fructosediphosphates / Q000627:therapeutic use*; D007668:Kidney / Q000737:chemistry; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
chemical_list: D004731:Endotoxins; D005635:Fructosediphosphates; D000255:Adenosine Triphosphate; D000254:Sodium-Potassium-Exchanging ATPase; C029063:fructose-1,6-diphosphate