Coexpression by vaccinia virus recombinants of equine herpesvirus 1 glycoproteins gp13 and gp14 results in potentiated immunity.
The equine herpesvirus 1 glycoprotein 14 (EHV-1 gp14) gene was cloned, sequenced, and expressed by vaccinia virus recombinants. Recombinant virus vP613 elicited the production of EHV-1-neutralizing antibodies in guinea pigs and was effective in protecting hamsters from subsequent lethal EHV-1 challenge. Coexpression of EHV-1 gp14 in vaccinia virus recombinant vP634 along with EHV-1 gp13 (P. Guo, S. Goebel, S. Davis, M. E. Perkus, B. Languet, P. Desmettre, G. Allen, and E. Paoletti, J. Virol. 63:4189-4198, 1989) greatly enhanced the protective efficacy in the hamster challenge model over that obtained with single recombinants. The inoculum doses (log10) required for protection of 50% of hamsters were 6.1 (EHV-1 gp13), 5.2 (EHV-1 gp14), and less than 3.6 (vaccinia virus recombinant expressing both EHV-1 glycoproteins [gp13 and gp14]).
Guo
P X
PX
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201.
Goebel
S
S
Perkus
M E
ME
Taylor
J
J
Norton
E
E
Allen
G
G
Languet
B
B
Desmettre
P
P
Paoletti
E
E
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000917:Antibody Formation; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D006224:Cricetinae; D015870:Gene Expression; D005814:Genes, Viral; D006168:Guinea Pigs; D006564:Herpesviridae / Q000235:genetics*; D006566:Herpesviridae Infections / Q000276:immunology / Q000517:prevention & control*; D004861:Herpesvirus 1, Equid / Q000235:genetics* / Q000276:immunology; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D015345:Oligonucleotide Probes; D014616:Vaccinia virus / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology; D015678:Viral Structural Proteins / Q000235:genetics*; D014765:Viral Vaccines / Q000008:administration & dosage*
D000914:Antibodies, Viral; D008562:Membrane Glycoproteins; D015345:Oligonucleotide Probes; D014759:Viral Envelope Proteins; D015678:Viral Structural Proteins; D014765:Viral Vaccines; C063677:gp14 protein, equine herpesvirus 1