Relationship between 3H-imipramine binding, 14C-serotonin uptake, and the surface area of blood platelets.
1. The relationship between 14C-serotonin uptake, 3H-imipramine binding and surface area was studied in blood platelets. 2. Platelet rich plasma from 5 normal subjects was divided into 5 size subfractions by differential centrifugation and the total surface areas computed from platelet size profiles. 3. Linear relationships were found between both uptake and binding as a function of surface area regardless of platelet size. 4. The results suggest that platelet heterogeneity may contribute to the variability of uptake measurements which are commonly expressed on a per platelet basis. In contrast, the importance of heterogeneity to binding measurements, which are expressed per unit protein, may have been previously overestimated.
Demet
E M
EM
Department of Psychiatry and Human Behavior, University of California, Irvine.
Chicz-Demet
A
A
Shaffer
E
E
Ocariz
J
J
D001692:Biological Transport; D001792:Blood Platelets / Q000378:metabolism* / Q000648:ultrastructure; D002250:Carbon Radioisotopes; D002352:Carrier Proteins; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D007099:Imipramine / Q000097:blood*; D007700:Kinetics; D011865:Radioisotope Dilution Technique; D011955:Receptors, Drug; D017981:Receptors, Neurotransmitter / Q000378:metabolism; D012016:Reference Values; D012701:Serotonin / Q000097:blood*; D014316:Tritium
D002250:Carbon Radioisotopes; D002352:Carrier Proteins; D011955:Receptors, Drug; D017981:Receptors, Neurotransmitter; C027775:imipramine receptor; D014316:Tritium; D012701:Serotonin; D007099:Imipramine