New evidence for the precursor role of 14 S subunits in poliovirus morphogenesis.
In poliovirus-infected HeLa cells incubated as 30 degrees, 14 S subunits are selectively labeled and no virions are assembled. When the temperature is subsequently shifted to 37 degrees, the radioactivity of the 14 S material is transferred mainly to virions, showing that 14 S subunits are virion precursors. When 14 S subunits synthesized at 30 degrees are incubated in vitro at 37 degrees, they assemble to empty capsids.
Rombaut
B
B
Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Belgium.
Vrijsen
R
R
Boeyé
A
A
D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D009024:Morphogenesis; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D017955:Poliovirus / Q000235:genetics* / Q000502:physiology; D012367:RNA, Viral / Q000096:biosynthesis / Q000302:isolation & purification; D013696:Temperature; D014764:Viral Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D014771:Virion / Q000235:genetics / Q000502:physiology
D009363:Neoplasm Proteins; D012367:RNA, Viral; D014764:Viral Proteins