A monoclonal anti-peptide antibody recognizes the adrenocorticotropic receptor.
We have produced a monoclonal antibody that specifically recognizes the adrenocorticotropic receptor on rat adrenal cells. The immunogen was designed from an RNA sequence complementary to the mRNA coding for ACTH1-24. This complementary peptide, termed HTCA, has been shown to specifically bind ACTH and was proposed to mimic the ACTH binding site of the hormone receptor. The monoclonal anti-HTCA antibody recognized a restricted domain of the HTCA peptide, bound to Y-1 adrenal cells with a KD of 1.8 nM, and blocked the binding of 125I-ACTH to rat adrenal cells. These findings show that anti-HTCA competes with ACTH for binding to the ACTH receptor.
Clarke
B L
BL
University of Alabama, Department of Physiology and Biophysics, Birmingham.
Bost
K L
KL
D000313:Adrenal Medulla / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism*; D000918:Antibody Specificity; D001667:Binding, Competitive; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009842:Oligopeptides / Q000138:chemical synthesis / Q000276:immunology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D018043:Receptors, Corticotropin; D011979:Receptors, Pituitary Hormone / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D009842:Oligopeptides; D012333:RNA, Messenger; D018043:Receptors, Corticotropin; D011979:Receptors, Pituitary Hormone; D000324:Adrenocorticotropic Hormone