Synthesis of the herpes simplex virus type 1 trans-activator Vmw65 in insect cells using a baculovirus vector.
Vmw65, the Herpes Simplex Virus trans-activator of immediate-early genes, was expressed in insect cells using a recombinant baculovirus expression vector and partially purified. Insect cell-derived Vmw65 was shown to be indistinguishable from authentic Vmw65 present in purified HSV-1 virions based on electrophoretic mobility, immunoreactivity with a monoclonal antibody, and ability to interact with cellular factors to form a protein/DNA complex with oligonucleotides containing a TAATGARAT element.
Capone
J P
JP
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada.
Werstuck
G
G
D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000378:metabolism; D005822:Genetic Vectors; D007304:Insect Viruses / Q000235:genetics*; D007313:Insecta / Q000235:genetics*; D007915:Lepidoptera / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids; D018139:Simplexvirus / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics*
D004279:DNA, Viral; D015534:Trans-Activators