Biochemical characterization of a putative axonal guidance molecule of the chick visual system.
Temporal retinal axons growing in vitro on carpets of tectal membranes are deflected by cell membranes of posterior tectum. The activity responsible for this deflection can be abolished by antibodies raised against tectal membranes and the corresponding Fab fragments. Analysis of tectal membranes by two-dimensional gel electrophoresis and immunoblotting reveals a 33 kd glycoprotein that has a higher concentration in posterior than in anterior tectum. Its expression is developmentally regulated, and it is sensitive to phosphatidylinositol-specific phospholipase C. These are properties expected for a molecule responsible for the phenomena observed in experiments on in vitro guidance of retinal axons.
Stahl
B
B
Max-Planck-Institut für Entwicklungsbiologie, Tübingen, Federal Republic of Germany.
Müller
B
B
von Boxberg
Y
Y
Cox
E C
EC
Bonhoeffer
F
F
D000818:Animals; D000906:Antibodies / Q000502:physiology; D001369:Axons / Q000187:drug effects / Q000502:physiology*; D002642:Chick Embryo; D007106:Immune Sera / Q000502:physiology; D007140:Immunoglobulin Fab Fragments / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000378:metabolism / Q000502:physiology*; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000494:pharmacology; D012160:Retina / Q000378:metabolism* / Q000648:ultrastructure; D013477:Superior Colliculi / Q000378:metabolism*
D000906:Antibodies; D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D008562:Membrane Glycoproteins; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase