Effects of fluoride on matrix proteins and their properties in rat secretory enamel.
This publication concerns the selective adsorption of rat enamel proteins onto hydroxyapatite, their solubility in aqueous solutions, and the effect that systemic fluoride has on these properties. The enamel proteins used as adsorbates were extracted in 0.5 mol/L acetic acid from the secretory enamel of the upper and lower incisors of SD rats (females, 200-220 g body weight). Equilibration of the proteins with hydroxyapatite was performed in two solutions: (i) 50 mmol/L acetate buffer at pH 6.0 and 0 degrees C, and (ii) 50 mmol/L Tris buffer containing 4 mol/L guanidine at pH 7.4 and room temperature. Enamel was dissected from animals, which were given either de-ionized water (control group) or water containing 25, 50, 75, or 100 ppm fluoride as NaF for four weeks. From these enamel samples, the proteins were extracted in sequence with 160 mmol/L NaCl and 3 mmol/L phosphate (pH 7.3), 50 mmol/L carbonate buffer (pH 10.8), and finally, with 0.5 mol/L acetic acid for dissolution of the enamel mineral. The F, Ca, and P contents of the various enamel samples were determined.(ABSTRACT TRUNCATED AT 250 WORDS)
Aoba
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Forsyth Dental Center, Boston, Massachusetts 02115.
Moreno
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Tanabe
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Fukae
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D000327:Adsorption; D000566:Amelogenesis; D053523:Amelogenin; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D003743:Dental Enamel / Q000032:analysis / Q000187:drug effects* / Q000502:physiology; D003746:Dental Enamel Proteins / Q000032:analysis / Q000502:physiology*; D017886:Durapatite; D005260:Female; D005459:Fluorides / Q000494:pharmacology*; D006882:Hydroxyapatites; D051381:Rats; D011919:Rats, Inbred Strains
D053523:Amelogenin; C507201:Amelx protein, rat; D000596:Amino Acids; D003746:Dental Enamel Proteins; D006882:Hydroxyapatites; D017886:Durapatite; D005459:Fluorides