Further investigation into the ocular effects of prostaglandin E2, leukotriene B4 and formyl-methionyl-leucyl phenylalanine.
The effect of indomethacin and dexamethasone on the inflammatory response to intraocularly injected prostaglandin E2 (PGE2), leukotriene B4 (LTB4), and formyl-methionyl-leucyl phenylalanine (FMLP) has been examined in rabbits. PGE2, in nanogram quantities, dose-dependently increased intraocular pressure and protein concentration in the aqueous humor. These responses were not affected by an anti-inflammatory dose of dexamethasone, but indomethacin had a small insignificant inhibitory effect. LTB4 and FMLP caused substantial leukocyte accumulation in the anterior chamber and did not disrupt the blood-aqueous barrier. Neither indomethacin nor dexamethasone had any effect on the leukocyte response to LTB4 However, dexamethasone significantly reduced the FMLP-induced leukocyte response. These results suggest that PGE2 and LTB4 do not elicit a further release of AA metabolites and that their actions are direct. However, the chemofactic response of polymorphonuclear leukocytes to FMLP appears to be mediated by lipoxygenase products.
Bhattacherjee
P
P
KY Lions Eye Research Institute, University of Louisville 40292.
Paterson
C A
CA
D000818:Animals; D001082:Aqueous Humor / Q000187:drug effects / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D015232:Dinoprostone / Q000494:pharmacology*; D005136:Eye Proteins / Q000378:metabolism; D007213:Indomethacin / Q000494:pharmacology; D007429:Intraocular Pressure / Q000187:drug effects*; D007975:Leukotriene B4 / Q000494:pharmacology*; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects*; D011817:Rabbits
D005136:Eye Proteins; D007975:Leukotriene B4; D009240:N-Formylmethionine Leucyl-Phenylalanine; D003907:Dexamethasone; D015232:Dinoprostone; D007213:Indomethacin