[Substrate-induced cAMP signals in wild-type and mutant strains of Saccharomyces cerevisiae].
Addition of glucose or other substrates to starved Saccharomyces cerevisiae cells triggers a cyclic AMP signal which induces the protein phosphorylating cascade. Before the addition of various substrates the wild-type and mutant yeast strains were arrested at the G1 phase of the cell division cycle by transferring the cells, grown at 26 degrees C to 36 degrees C in a synthetic medium without any substrate. After the temperature shift back to 26 degrees C different substrates were added and the cAMP levels were measured. The highest cAMP levels were observed immediately after the addition of the substrates. A relationship between the maximum growth rate of the individual strains or mutants at a given substrate and the intracellular cAMP level is discussed.
Sachse
O
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Biotechnikum der Martin-Luther-Universität Halle, DDR.
D050260:Carbohydrate Metabolism; D002453:Cell Cycle; D000242:Cyclic AMP / Q000502:physiology*; D000431:Ethanol / Q000378:metabolism; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D013696:Temperature; D013997:Time Factors
D000431:Ethanol; D000242:Cyclic AMP
Substratinduzierte cAMP-Signale in Wildstämmen und Mutanten von Saccharomyces cerevisiae.