Inhibited enzyme electrodes. Part 1: Theoretical model.
A theoretical model is developed for an electrochemical sensor for toxic substances which works by measuring the inhibition of the enzyme activity. The enzyme is assumed to follow Michaelis-Menten kinetics and the diffusion kinetic equation describing the concentration profile of the enzyme's substrate in the electrolyte layer between the electrode and the membrane covering the electrode is solved. A complete set of analytical solutions is found which corresponds to a number of different rate limiting processes. The set of solutions is described in a case diagram. The use of cytochrome oxidase in particular is discussed.
Albery
W J
WJ
Department of Chemistry, Imperial College, London, UK.
Cass
A E
AE
Shu
Z X
ZX
D015374:Biosensing Techniques; D004058:Diffusion; D003576:Electron Transport Complex IV / Q000037:antagonists & inhibitors; D007700:Kinetics; D008962:Models, Theoretical; D014118:Toxins, Biological / Q000032:analysis
D014118:Toxins, Biological; D003576:Electron Transport Complex IV