Pretreatment of urine samples for the analysis of 11-nor-delta 9-tetrahydrocannabinol-9-carboxylic acid using solid-phase extraction.
An extraction and concentration procedure using a unique solid-phase extraction tube was developed for the analysis of 11-nor-delta 9-tetrahydrocannabinol-9-carboxylic acid in urine. Absolute and relative recoveries of the drug metabolite were found to be greater than 85% and 92%, respectively. The extraction procedure allows quantitation of the drug metabolite by GC/MS, HPLC with UV detection, or GC with flame ionization detection. The extraction procedure was verified with urine samples obtained from marijuana users as well as spiked urine samples.
Parry
R C
RC
Supelco, Inc., Bellefonte, PA 16823.
Nolan
L
L
Shirey
R E
RE
Wachob
G D
GD
Gisch
D J
DJ
D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D013759:Dronabinol / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D006868:Hydrolysis; D013058:Mass Spectrometry; D013056:Spectrophotometry, Ultraviolet; D015813:Substance Abuse Detection; D014297:Trimethylsilyl Compounds / Q000032:analysis / Q000138:chemical synthesis
D014297:Trimethylsilyl Compounds; C016780:11-nor-delta(9)-tetrahydrocannabinol-9-carboxylic acid; D013759:Dronabinol