title: The preference for a 3' homologous end is intrinsic to RecA-promoted strand exchange.
abstract: The recA protein (RecA) promotes DNA pairing and strand exchange optimally in the presence of single-stranded binding protein (SSB). Under these conditions, 3' homologous ends are essential for stable joint molecule formation between linear single-stranded DNA (ssDNA) and supercoiled DNA (i.e. 3' ends are 50-60 times more reactive than 5' ends). Linear ssDNAs with homology at the 5' end do not participate in pairing. In the absence of SSB, the strand exchange reaction is less efficient; however, linear ssDNAs with 3' end homology are still 5- to 10-fold more reactive than those with 5' end homology. The preference for a 3' homologous end in the absence of SSB suggests that this is an intrinsic property of RecA-promoted strand exchange. The preferential reactivity of 3' homologous ends is likely to be a consequence of the polarity of polymerization of RecA on ssDNA. Specifically, since RecA polymerizes in the 5'----3' direction, 3' ends are more likely to be coated with RecA and, hence, will be more reactive than 5' ends.
authors.affiliation: Department of Biochemistry, Stanford University School of Medicine, California 94305-5307.
authors.forename: B B
authors.initials: BB
authors.lastname: Konforti
authors.forename: R W
authors.initials: RW
authors.lastname: Davis
mesh_terms: D004270:DNA, Circular / Q000378:metabolism; D004278:DNA, Superhelical / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D009690:Nucleic Acid Conformation; D010957:Plasmids; D011938:Rec A Recombinases / Q000302:isolation & purification / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D013379:Substrate Specificity
chemical_list: D004270:DNA, Circular; D004278:DNA, Superhelical; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D011938:Rec A Recombinases