Modification of the gluconeogenic/glycolytic balance in isolated perfused liver of myeloproliferative leukemia infected mice: a 13C NMR study.
1. Isolated perfused livers from starved mice inoculated with myeloproliferative leukemia virus exhibited similar rates of consumption of [3-13C]alanine and of synthesis of glutamine and glutamate labeled at the C-2 or C-3 positions as livers from uninfected mice. 2. Leukemic livers also formed glutamine and glutamate labeled at the C-4 position. This is related to their lower content of triglyceride as compared to that of control livers which do not produce these isotopomers. 3. The glucose synthesis rate was much lower in livers from leukemic mice. This is explained by the glycolytic properties of the leukemic infiltrating cells.
Nedelec
J F
JF
Institut Curie, Section de Biologie, U.219 INSERM, Centre Universitaire, Orsay, France.
Mégnin
F
F
Patry
J
J
Jullien
P
P
Lhoste
J M
JM
Dimicoli
J L
JL
D000409:Alanine / Q000378:metabolism; D000818:Animals; D002247:Carbon Isotopes; D004734:Energy Metabolism / Q000502:physiology; D005943:Gluconeogenesis / Q000502:physiology*; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis / Q000502:physiology*; D066298:In Vitro Techniques; D009052:Leukemia Virus, Murine; D007942:Leukemia, Experimental / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D010477:Perfusion
D002247:Carbon Isotopes; D005947:Glucose; D000409:Alanine