Expression of the IGF-II/mannose-6-phosphate receptor mRNA and protein in the developing rat.
The insulin-like growth factor II (IGF-II) receptor is identical to the mannose-6-phosphate receptor (M-6-P), but its role as a somatomedin transducer is uncertain. IGF-II/M-6-P receptor expression was studied by in situ hybridization (ISH) in the developing rat. Expression occurs in extra-embryonic membranes at the time of IGF-II mRNA induction and later at paracrine/autocrine sites of IGF-II action (skeletal muscle and perichondrium) in the embryo. Highest levels of receptor mRNA occur in heart and major vessels. Postnatally transcription is strongly down-regulated. This suggests a role for the IGF-II/M-6-P receptor in IGF-II action or turnover during development distinct from its role in lysosomal transport.
Senior
P V
PV
Department of Anatomy, University of Leicester, UK.
Byrne
S
S
Brammar
W J
WJ
Beck
F
F
D000818:Animals; D015152:Blotting, Northern; D004622:Embryo, Mammalian / Q000378:metabolism*; D047108:Embryonic Development; D004707:Endoderm / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000378:metabolism*; D005865:Gestational Age; D008648:Mesoderm / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017527:Receptor, IGF Type 2; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000235:genetics*; D017451:Receptors, Somatomedin; D015017:Yolk Sac / Q000378:metabolism
D012333:RNA, Messenger; D017527:Receptor, IGF Type 2; D011956:Receptors, Cell Surface; D017451:Receptors, Somatomedin