title: Local immune reaction in syngeneic mice against tumorigenic and nontumorigenic BPV-transformed mouse cell lines.
abstract: The role of cytotoxic T cells in immune response to Bovine Papillomavirus type 1 (BPV1)-transformed mouse cell lines was assessed. The chromium release assay was used to follow the induction of cytotoxicity in local lymph nodes of syngeneic C57BL/6J (B6) mice after injection of BPV1-transformed cell lines tumorigenic in nude mice but tumorigenic or nontumorigenic in B6 mice. The nontumorigenic cell line B6B31.C-NuT.A induced cell line-specific cytotoxicity with a maximal activity on day 7 after subcutaneous inoculation of one million B6B31.C-NuT.A cells. After injection of nontumorigenic B6B31.J or tumorigenic B6B31.J-NuT.A or B6B31.A cell lines, only low levels of nonspecific cytotoxicity were observed. These results suggest a role for cytotoxic T cells only in rejection of B6B31.C-NuT.A cells. One reason for the poor immunogenicity could be the lack of transformation-induced/virus-specific antigens on most of the present BPV1-transformed cell lines. However, in a transplantation rejection experiment, protection was induced in B6 mice against a challenge of the highly tumorigenic B6B31.A-B6T.1 line by repeated injections of BPV1-transformed cells. These results suggest the expression of common transplantation rejection antigens on the three BPV1-transformed cell lines used in the immunization experiments.
authors.affiliation: Department of Clinical Microbiology, University of Kuopio, Finland.
authors.forename: A
authors.initials: A
authors.lastname: Laatikainen
authors.forename: H
authors.initials: H
authors.lastname: Sarkkinen
authors.forename: K
authors.initials: K
authors.lastname: Syrjänen
authors.forename: R
authors.initials: R
authors.lastname: Mäntyjärvi
mesh_terms: D000818:Animals; D052688:Bovine papillomavirus 1 / Q000472:pathogenicity*; D002472:Cell Transformation, Viral; D003602:Cytotoxicity, Immunologic; D006084:Graft Rejection; D007111:Immunity, Cellular; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology* / Q000382:microbiology; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*