The ability of granulocytes to generate superoxide anions and hypochlorite during phagocytosis: comparison of neonatal granulocytes with adult granulocytes.
The ability of granulocytes to generate superoxide anions (O2-) and hypochlorite (OCl-) during phagocytosis was investigated using peripheral blood samples from adults and cord blood samples from neonates, using the chemiluminescence probe cypiridina luciferin analog (CLA) for O2- generation and luminol (L) for OCl- generation. OCl- generation by granulocytes was also monitored by taurine chloramine formation. The chemiluminescence probe based upon CLA was highly specific for and sensitive to O2- and could be adopted to determine O2- generation in terms of xanthine oxidase units. The CLA-dependent chemiluminescence by cord blood granulocytes was significantly higher than that by normal adult granulocytes. Taurine chloramine formation was significantly correlated with the L-dependent chemiluminescence (L-CL). Thus, the L-CL is considered to be mainly involved in OCl- generated by phagocytizing granulocytes. L-CLs by cord blood granulocytes and normal adult granulocytes were essentially the same during phagocytosis.
Nishida
A
A
Department of Neonatology, Gunma Children's Medical Center, Hokkitsu, Japan.
Kimura
H
H
Sugioka
K
K
Nakano
M
M
D000328:Adult; D000375:Aging / Q000378:metabolism*; D000838:Anions / Q000378:metabolism*; D005312:Fetal Blood; D049454:Firefly Luciferin; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D006997:Hypochlorous Acid / Q000378:metabolism*; D007231:Infant, Newborn; D008163:Luminescent Measurements; D010587:Phagocytosis; D011719:Pyrazines; D013481:Superoxides / Q000378:metabolism*
D000838:Anions; D011719:Pyrazines; C048193:2-methyl-6-phenyl-3,7-dihydroimidazo(1,2-a)pyrazin-3-one; D013481:Superoxides; D049454:Firefly Luciferin; D006997:Hypochlorous Acid