Asbestos fibers induce release of collagenase by human polymorphonuclear leukocytes.
The asbestos fibers chrysotile and crocidolite cause a dose-dependent release of specific granule collagenase by human polymorphonuclear leukocytes (PMNL). Release of azurophil granule elastase was induced by the asbestos fibers at higher concentrations, suggesting that asbestos fibers primarily cause the release of specific granule contents of human PMNL. Wollastonite, a fibrous silicate mineral, causes a weaker collagenase release and no elastase release. The collagenase was released in inactive, latent form. Carboxymethyl cellulose (CMC), an agent known to blunt chrysotile-induced hemolysis and production of reactive oxygen metabolites by human PMNL, specifically inhibits chrysotile-induced release of collagenase. Chrysotile asbestos was found to bind the PMNL serine proteinase cathepsin G. A role of collagenase release, production of reactive oxygen metabolites and cathepsin G binding by chrysotile for the perpetuation of the asbestos-induced alveolitis is suggested.
Hedenborg
M
M
Institute of Occupational Health, Helsinki, Finland.
Sorsa
T
T
Lauhio
A
A
Klockars
M
M
D001194:Asbestos / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D017638:Asbestos, Crocidolite; D017632:Asbestos, Serpentine; D017610:Calcium Compounds; D002266:Carboxymethylcellulose Sodium / Q000494:pharmacology; D056649:Cathepsin G; D002403:Cathepsins / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D003012:Microbial Collagenase / Q000378:metabolism*; D009504:Neutrophils / Q000187:drug effects* / Q000201:enzymology; D010196:Pancreatic Elastase / Q000378:metabolism; D012697:Serine Endopeptidases; D017640:Silicates; D012824:Silicic Acid / Q000494:pharmacology
D017632:Asbestos, Serpentine; D017610:Calcium Compounds; D017640:Silicates; D017638:Asbestos, Crocidolite; D001194:Asbestos; D012824:Silicic Acid; D007770:L-Lactate Dehydrogenase; D002403:Cathepsins; D012697:Serine Endopeptidases; C534094:CTSG protein, human; D056649:Cathepsin G; D010196:Pancreatic Elastase; D003012:Microbial Collagenase; D002266:Carboxymethylcellulose Sodium; C031293:calcium silicate