Okadaic acid stimulates the activity of microtubule associated protein kinase in PC-12 pheochromocytoma cells.
PC-12 pheochromocytoma cells contain a growth factor-sensitive protein kinase that phosphorylates microtubule associated protein 2 (MAP-2). This MAP kinase is also activated by the protein phosphatase inhibitor okadaic acid (OA). Additionally, OA potentiates the NGF-dependent activation of MAP kinase, but causes only a modest potentiation (20%) of the maximal activation observed with EGF. Since OA is a specific serine/threonine phosphatase inhibitor, these results suggest that serine/threonine phosphorylation may be involved in the hormonal regulation of MAP kinase.
Miyasaka
T
T
Laboratory of Molecular Oncology, Rockefeller University, New York, New York 10021.
Miyasaka
J
J
Saltiel
A R
AR
D000310:Adrenal Gland Neoplasms; D000818:Animals; D001921:Brain / Q000201:enzymology; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004988:Ethers, Cyclic / Q000494:pharmacology*; D009414:Nerve Growth Factors / Q000494:pharmacology; D019319:Okadaic Acid; D010673:Pheochromocytoma; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D012694:Serine / Q000378:metabolism; D013552:Swine; D013912:Threonine / Q000378:metabolism; D014407:Tumor Cells, Cultured
D004988:Ethers, Cyclic; D009414:Nerve Growth Factors; D019319:Okadaic Acid; D013912:Threonine; D012694:Serine; D004815:Epidermal Growth Factor; D011494:Protein Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases