Transport and metabolism of polyamines in human lymphocytes.
1. Polyamines are taken up by human peripheral lymphocytes in a concentration, time and pH dependent manner, with an energy-dependent transport system. 2. Each polyamine inhibits the uptake of the others, with the exception of putrescine. Spermine appears to have the highest affinity for the transporter/s. 3. Inhibition by ouabain, amiloride and vanadate suggests that the transport is dependent on Na+. 4. Polyamine content inside the cells increases by ca 6 and 3 times respectively during incubation with spermidine or spermine. 5. The incorporated polyamines are partially transformed into each other.
Colombatto
S
S
Dipartimento di Medicina e Oncologia Sperimentale, Università di Torino, Italia.
Fasulo
L
L
Fulgosi
B
B
Grillo
M A
MA
D000584:Amiloride / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008214:Lymphocytes / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology; D011073:Polyamines / Q000378:metabolism* / Q000494:pharmacology; D011700:Putrescine / Q000378:metabolism / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors; D013095:Spermidine / Q000378:metabolism / Q000494:pharmacology; D013096:Spermine / Q000378:metabolism / Q000494:pharmacology; D014638:Vanadates / Q000494:pharmacology
D011073:Polyamines; D013096:Spermine; D014638:Vanadates; D010042:Ouabain; D000584:Amiloride; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D013095:Spermidine; D011700:Putrescine