title: Maturation-dependent changes of the rat reticulocyte energy metabolism and hormonal responsiveness.
abstract: Significant reduction of total and coupled respiration as well as an increase of uncoupled respiration characterize the maturation of rat reticulocytes. ATP declines by 45%, whereas ADP increases by 70% and the ATP/ADP ratio decreases from 7.6 to 3.3. At the same time the oxygen-delivery capacity of red blood cells increases by 37% due to an increase of 2.3-BPG level. In the youngest reticulocytes the basic level of cAMP is 2-fold higher, whereas the maximal (-)-isoproterenol-induced accumulation of cAMP is 8-fold higher than in the fraction of most mature reticulocytes. Evidence for the presence of functionally active glucagon-receptors in reticulocytes is presented, with maturational inactivation/disappearance of R2-glucagon receptors.
authors.affiliation: Institute of Physiology, Faculty of Medicine, University S. Marković, Kragujevac, Yugoslavia.
authors.forename: M M
authors.initials: MM
authors.lastname: Kostić
authors.forename: R V
authors.initials: RV
authors.lastname: Zivković
authors.forename: S M
authors.initials: SM
authors.lastname: Rapoport
mesh_terms: D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D004734:Energy Metabolism; D005934:Glucagon / Q000494:pharmacology*; D007545:Isoproterenol / Q000494:pharmacology*; D010101:Oxygen Consumption; D051381:Rats; D011919:Rats, Inbred Strains; D011964:Receptors, Gastrointestinal Hormone / Q000187:drug effects / Q000378:metabolism; D018027:Receptors, Glucagon; D012156:Reticulocytes / Q000187:drug effects / Q000378:metabolism*
chemical_list: D011964:Receptors, Gastrointestinal Hormone; D018027:Receptors, Glucagon; D005934:Glucagon; D000242:Cyclic AMP; D007545:Isoproterenol