title: Molecular phenotype of a pediatric small round cell tumor.
abstract: Molecular probes were used to characterize an unusual small round cell abdominal tumor arising from the fallopian tube of a 15-year-old girl. DNA and RNA extracted from the tumor and adjacent normal tissue was subjected to Southern and Northern blot analysis using a variety of different probes. N-myc oncogene RNA was greatly expressed in the tumor, but was not expressed in normal tissue or amplified in chromosomal DNA. Insulin-like growth factor II RNA was similarly overexpressed in the neoplasm, but not in normal tissue. While histopathologic studies could not distinguish between a neuroectodermal neoplasm and Wilms' tumor, electron microscopy and the pattern of gene expression was most consistent with Wilms' tumor.
authors.affiliation: Schneider Children's Hospital of Long Island Jewish Medical Center, New Hyde Park, New York.
authors.forename: I
authors.initials: I
authors.lastname: Bendit
authors.forename: J
authors.initials: J
authors.lastname: Johnston
authors.forename: E
authors.initials: E
authors.lastname: Valderrama
authors.forename: P
authors.initials: P
authors.lastname: Waber
authors.forename: P D
authors.initials: PD
authors.lastname: Nisen
mesh_terms: D000293:Adolescent; D015152:Blotting, Northern; D015139:Blotting, Southern; D004273:DNA, Neoplasm / Q000032:analysis; D005185:Fallopian Tube Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D005784:Gene Amplification; D016259:Genes, myc; D006801:Humans; D010641:Phenotype; D012334:RNA, Neoplasm / Q000032:analysis; D012186:Retroperitoneal Neoplasms / Q000235:genetics* / Q000473:pathology; D009396:Wilms Tumor / Q000235:genetics* / Q000473:pathology
chemical_list: D004273:DNA, Neoplasm; D012334:RNA, Neoplasm