title: [Influence of endotoxin on the synthesis of lipoxygenase products formed from arachidonic acid by rat alveolar macrophages].
abstract: Alveolar macrophages of normal and endotoxin--treated rats were stimulated in suspension by calcium ionophore A 23187. After solid phase extraction and isocratic separation by HPLC the lipoxygenase products (Leukotriene B4, 5-hydroxyeicosatetraenoic acid and 12-hydroxyeicosatetraenioc acid) were quantified. Unlike alveolar macrophages of control animals, alveolar macrophages of endotoxin treated rats showed an increased synthesis of lipoxygenase products (Leukotriene B4: 1.6fold; 5-hydroxyeicosatetraenoic acid: 2.1fold; 12-hydroxyeicosatetraenoic acid: 1.3fold). Our results suggest that in vivo endotoxin disturbs the mechanisms of arachidonic acid liberation in alveolar macrophages.
authors.affiliation: Medizinische Akademie Carl Gustav Carus Dresden Institut für Pathologische Biochemie, DDR.
authors.forename: B
authors.initials: B
authors.lastname: Lehmann
authors.forename: A
authors.initials: A
authors.lastname: Kämpf
authors.forename: D W
authors.initials: DW
authors.lastname: Scheuch
mesh_terms: D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D004731:Endotoxins / Q000502:physiology*; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism; D007975:Leukotriene B4 / Q000378:metabolism; D008084:Lipoxygenase / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000187:drug effects / Q000378:metabolism*; D051381:Rats
chemical_list: D001095:Arachidonic Acids; D004731:Endotoxins; D006893:Hydroxyeicosatetraenoic Acids; D007975:Leukotriene B4; D000001:Calcimycin; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D008084:Lipoxygenase
vernacular_title: Zum Einfluss von Endotoxin auf die Synthese von Lipoxygenaseprodukten der Arachidonsäure in Alveolarmakrophagen der Ratte.