NMR investigation of cell cultures: early detection of infection by Herpes simplex virus type 2 and transformation.
The water proton Nuclear Magnetic Resonance (NMR) spin-lattice relaxation rates were measured in suspension of uninfected and Herpes simplex virus type 2-infected WI-38 diploid cells and shown to allow reliable detection of (i) the early signs of productive infection, and (ii) the tendency to undergo either spontaneous or virus-induced transformation. In addition, NMR investigation of several transformed cell lines currently and previously established from WI-38 cells clearly indicated that the NMR relaxation parameter is a suitable tool for assessment of the level of malignancy, as revealed by in vivo oncogenicity tests in immunosuppressed mice.
Valensin
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Istituto di Microbiologia, Università di Siena, Italy.
Bianchi Bandinelli
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ML
Zazzi
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Gaggelli
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E
Valensin
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D000818:Animals; D002460:Cell Line; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D003588:Cytopathogenic Effect, Viral; D004171:Diploidy; D005347:Fibroblasts; D006801:Humans; D007165:Immunosuppression Therapy; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D018139:Simplexvirus / Q000302:isolation & purification* / Q000502:physiology