Cell cycle dependence of chloride permeability in normal and cystic fibrosis lymphocytes.
Cystic fibrosis (CF) is a genetic disease characterized by abnormal regulation of epithelial cell chloride channels. Nonepithelial cells, including lymphocytes and fibroblasts, may exhibit a similar defect. Two independent techniques were used to assess the macroscopic chloride permeability (PCl) of freshly isolated B lymphocytes and of B and T lymphocyte cell lines. Values for PCl increased specifically during the G1 phase of the cell cycle and could be further enhanced by increasing intracellular adenosine 3',5'-monophosphate (cAMP) or calcium. In lymphocytes from CF patients, regulation of PCl during the cell cycle and by second messengers was absent. Characterization of the cell cycle-dependent expression of the chloride permeability defect in lymphocytes from CF patients increases the utility of these cells in the analysis of the functional consequences of mutations in the CF gene.
Bubien
J K
JK
Department of Physiology and Biophysics, University of Alabama, Birmingham 35294.
Kirk
K L
KL
Rado
T A
TA
Frizzell
R A
RA
D001402:B-Lymphocytes / Q000502:physiology; D002118:Calcium / Q000502:physiology; D002453:Cell Cycle; D002460:Cell Line; D002463:Cell Membrane Permeability; D002712:Chlorides / Q000378:metabolism*; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000494:pharmacology / Q000502:physiology; D003550:Cystic Fibrosis / Q000097:blood*; D004553:Electric Conductivity; D006801:Humans; D007399:Interphase; D015759:Ionomycin / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology*; D008856:Microscopy, Fluorescence; D015290:Second Messenger Systems; D013601:T-Lymphocytes / Q000502:physiology; D013873:Thionucleotides / Q000494:pharmacology
D002712:Chlorides; D013873:Thionucleotides; C015929:8-((4-chlorophenyl)thio)cyclic-3',5'-AMP; D015759:Ionomycin; D000242:Cyclic AMP; D002118:Calcium