Construction and analysis of linking libraries from the mouse X chromosome.
A hybrid cell line containing the mouse X chromosome on a human background has been used to construct linking libraries from the mouse X chromosome, and approximately 250 unique EagI and NotI clones have been identified. Seventy-three clones have been sublocalized onto the X chromosome using interspecific Mus spretus/Mus domesticus crosses and a panel of somatic cell hybrids carrying one-half of reciprocal X-autosome translocations. The average spacing of the linking clones mapped to date is about one every 2 Mb of DNA. Two clones from the central region of the chromosome have been physically linked by pulsed-field gel electrophoresis. A large number of clones contain conserved sequences, indicating the presence of CpG-rich island-associated genes. The clones isolated from these libraries provide a valuable resource for comparative mapping between man and mouse X chromosomes, isolation of X-linked disease loci of interest by reverse genetics, and analysis of the long-range structure and organization of the chromosome.
Brockdorff
N
N
Section of Comparative Biology, MRC Clinical Research Centre, Harrow, Middlesex, United Kingdom.
Montague
M
M
Smith
S
S
Rastan
S
S
D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D015723:Gene Library; D008040:Genetic Linkage; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D009115:Muridae / Q000235:genetics; D010375:Pedigree; D014178:Translocation, Genetic; D014960:X Chromosome
D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases