Induction of immune response and protection from equine viral arteritis (EVA) by formalin inactivated-virus vaccine for EVA in horses.
Thirty-nine horses included 3 pregnant mares were examined by inoculating with formalin inactivated-virus vaccine for EVA. Antibody response of horses after one dose vaccination was somewhat poor and 50% effective inoculum dose of the vaccine should be included 10(8.4) pfu of virus before inactivation. After 2 doses given at an interval of 4 weeks, the horses developed such high titer of SN antibody as up to 1:5,120. The SN titer declined rather rapidly, but supplemental administration of the vaccine at an interval of more than 2 months elicited a prompt antibody response and SN titers persisted as 1:80 to 1:320 at 6 months after the administration. Therefore, supplemental administration of the vaccine as booster every 6 months or 1 year would be capable of maintaining high titer of SN antibody. The inactivated-virus vaccine prevented horses from clinical disease of EVA and protected pregnant mares from abortion by challenge exposure with virulent virus. Fifty percent protective dose in SN titer of 1:43 was confirmed by clinical signs and viremia.
Fukunaga
Y
Y
Epizootic Research Station, Equine Research Institute, Japan Racing Association, Tochigi.
Wada
R
R
Matsumura
T
T
Sugiura
T
T
Imagawa
H
H
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D001167:Arteritis / Q000517:prevention & control / Q000662:veterinary*; D007240:Equartevirus / Q000276:immunology*; D005260:Female; D006734:Horse Diseases / Q000517:prevention & control*; D006736:Horses; D011247:Pregnancy; D012328:RNA Viruses / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; D014777:Virus Diseases / Q000517:prevention & control / Q000662:veterinary*
D000914:Antibodies, Viral; D015164:Vaccines, Inactivated; D014765:Viral Vaccines