title: [The production of anti-HBc IgM antibodies by human lymphocytes transformed by the Epstein-Barr virus].
abstract: In transformation by Epstein-Barr virus of lymphocytes derived from patients with acute hepatitis B, continuous cell cultures were obtained which produced anti-HBc IgM antibodies. These cell lines underwent from 50 to 150 passages. The level of the specific immunoglobulin production was shown to have a trend to decline; however, after cloning the antibody production became more stable. The antibodies produced by the cloned lymphocyte culture could not neutralize the antigen (unlike polyclonal antibodies) which indicated a high efficacy of cloning. When the solid phase was sensitized with produced immunoglobulins, specific activity of binding of HBc antigen detectable by anti-HBc conjugate with horseradish peroxidase was demonstrated. The study of sedimentation properties of antibodies produced by transformed lymphocytes showed their sedimentation constant to be 19S.
authors.forename: A V
authors.initials: AV
authors.lastname: Gurov
authors.forename: M O
authors.initials: MO
authors.lastname: Favorov
authors.forename: T L
authors.initials: TL
authors.lastname: Iashina
authors.forename: E Z
authors.initials: EZ
authors.lastname: Gol'dberg
authors.forename: T M
authors.initials: TM
authors.lastname: Khizhniakova
authors.forename: V A
authors.initials: VA
authors.lastname: Anan'ev
mesh_terms: D002472:Cell Transformation, Viral / Q000276:immunology*; D002999:Clone Cells / Q000276:immunology; D006510:Hepatitis B Antibodies / Q000032:analysis / Q000096:biosynthesis*; D006512:Hepatitis B Core Antigens / Q000276:immunology*; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis / Q000096:biosynthesis*; D008214:Lymphocytes / Q000276:immunology*
chemical_list: D006510:Hepatitis B Antibodies; D006512:Hepatitis B Core Antigens; D007075:Immunoglobulin M
vernacular_title: Produktsiia anti-HBc IgM-antitel limfotsitami cheloveka, transformirovannymi virusom Epshteĭna--Barr.