Estimation of 140S particles in foot-and-mouth disease virus (FMDV) vaccine by using the computer analyzing system.
The quantity of 140S particles in inactivated foot-and-mouth disease virus (FMDV) vaccine samples produced in Foot-and-Mouth Disease Vaccine Production Center (FMD Vaccine Production Center) in Thailand was estimated by the sucrose gradient ultracentrifugation and optical density analysis by using the computer applying system. The soft ware; Chromato Data System (CDS) (Nihon Chromato Works Co., Ltd. Japan) which is prepared for the analysis of chromatography, was applied for the estimation of 140S particles in FMDV vaccine. The quantity of 140S particles in each vaccine sample measured by CDS was mostly ranged from 2-4 micrograms/ml and this quantity was consistent with the results of the other reports. This method is considered to be the available method for estimation of 140S particles in FMDV vaccine as routine assay.
Shirai
J
J
Poultry Disease Laboratory, National Institute of Animal Health, Gifu, Japan.
Chatchawanchonteera
A
A
Sinsuwongwat
W
W
Makarasen
P
P
Sugimura
T
T
D005537:Aphthovirus / Q000276:immunology*; D003201:Computers; D014765:Viral Vaccines / Q000032:analysis*
D014765:Viral Vaccines