Modulation of levels of messenger RNA for tissue-type plasminogen activator in rat Sertoli cells, and levels of messenger RNA for plasminogen activator inhibitor in testis peritubular cells.
Messenger RNA for tissue-type plasminogen activator has been detected in RNA extracts from rat Sertoli cells in culture. Relative levels are increased in Sertoli cells stimulated by follicle-stimulating hormone or by dibutyryl cyclic AMP (dbcAMP) and decreased in cells maintained in the presence of transforming growth factor beta, type 1 (TGF-beta 1). Messenger RNA for plasminogen activator inhibitor, type 1 (PAI-1) has been detected in RNA extracts from rat peritubular myoid cells. Relative levels are increased in peritubular cells stimulated by TGF-beta 1, and decreased by the presence of dbcAMP in the medium. Data are interpreted to indicate that net protease activities in the seminiferous tubule are regulated at transcriptional levels by endocrine and paracrine agents.
Nargolwalla
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C
Banting and Best Department of Medical Research, University of Toronto, Ontario, Canada.
McCabe
D
D
Fritz
I B
IB
D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000494:pharmacology; D003720:Densitometry; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D008297:Male; D015849:Plasminogen Inactivators / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012708:Sertoli Cells / Q000378:metabolism*; D013737:Testis / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors; D010959:Tissue Plasminogen Activator / Q000235:genetics*; D015291:Transforming Growth Factors / Q000494:pharmacology
D015849:Plasminogen Inactivators; D012333:RNA, Messenger; D015291:Transforming Growth Factors; D005640:Follicle Stimulating Hormone; D000242:Cyclic AMP; D010959:Tissue Plasminogen Activator