The effect of human cytomegalovirus on the production and biologic action of interleukin-1.
The effect of mycoplasma-free human cytomegalovirus (HCMV) on the production and biologic activity of interleukin-1 (IL-1) from peripheral blood monocytes was examined. The use of biologic thymocyte assays revealed a time-dependent decrease in the IL-1 activity of both HCMV-challenged and control monocytes after initiation of culture. A decrease in the amount of IL-1 beta secreted as measured by ELISA was also detected. The amount of IL-1 beta secreted by HCMV-challenged cells was always greater than that produced by control cultures at similar times. Despite containing higher levels of IL-1 beta, supernatants from challenged cells were markedly less effective in supporting thymocyte proliferation. It is proposed that this is due to the concomitant production of an inhibitor of IL-1 activity from HCMV-challenged monocyte cultures.
Moses
A V
AV
Department of Biology, University of Wollongong, New South Wales, Australia.
Garnett
H M
HM
D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000276:immunology; D008213:Lymphocyte Activation; D009000:Monocytes / Q000276:immunology / Q000382:microbiology*; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors
D007375:Interleukin-1