title: Calcium channel antagonist effects of spironolactone, an aldosterone antagonist.
abstract: The effect of spironolactone on the contractility of vascular smooth muscle cells and on calcium channel currents of isolated cells was investigated using the patch-clamp method. Concentrations of 10 and 60 microM of spironolactone resulted in 50 and 100% inhibition, respectively, of isometric contractions. Whereas fast calcium channel current was essentially unaffected by spironolactone, slow calcium channel current was inhibited 50% by 5 to 7 microM concentrations of the drug. These results suggest that spironolactone acts on slow calcium channels in a manner similar to that of calcium blockers.
authors.affiliation: Cellular Physiology and Molecular Pharmacology Laboratory, Centre National de la Recherche Scientifique, Bordeaux, France.
authors.forename: J
authors.initials: J
authors.lastname: Mironneau
mesh_terms: D000818:Animals; D002121:Calcium Channel Blockers; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D007537:Isometric Contraction / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D051381:Rats; D013148:Spironolactone / Q000494:pharmacology*
chemical_list: D002121:Calcium Channel Blockers; D015220:Calcium Channels; D013148:Spironolactone