Purification, by high-performance liquid chromatography, and characterization, by high-field 1H-n.m.r. spectroscopy, of two fucose-containing pentasaccharides of goat's milk.
Two fucose-containing pentasaccharides were isolated from goat's milk using a Bio-Gel P-4 column, followed by reverse-phase C-18 high-performance liquid chromatography. The structures of the pentasaccharides as characterized by high-field 1H-n.m.r. spectroscopy and enzymatic digestion were found to be [see text] and [see text].
Chaturvedi
P
P
Department of Biosciences and Biotechnology, University of Roorkee, India.
Sharma
C B
CB
D000818:Animals; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D005643:Fucose / Q000737:chemistry*; D006041:Goats; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008892:Milk / Q000737:chemistry*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D011522:Protons; D005644:alpha-L-Fucosidase
D009844:Oligosaccharides; D011522:Protons; D005643:Fucose; D005644:alpha-L-Fucosidase