The involvement of topoisomerases and DNA polymerase I in the mechanism of induced thermal and radiation resistance in yeast.
Either an ionizing radiation exposure or a heat shock is capable of inducing both thermal tolerance and radiation resistance in yeast. Yeast mutants, deficient in topoisomerase I, in topoisomerase II, or in DNA polymerase I, were used to investigate the mechanism of these inducible resistances. The absence of either or both topoisomerase activities did not prevent induction of either heat or radiation resistance. However, if both topoisomerase I and II activities were absent, the sensitivity of yeast to become thermally tolerant (in response to a heat stress) was markedly increased. The absence of only topoisomerase I activity (top1) resulted in the constitutive expression of increased radiation resistance equivalent to that induced by a heat shock in wild-type cells, and the topoisomerase I-deficient cells were not further inducible by heat. This heat-inducible component of radiation resistance (or its equivalent constitutive expression in top1 cells) was, in turn, only a portion of the full response inducible by radiation. The absence of polymerase I activity had no detectable effect on either response. Our results indicate that the actual systems that confer resistance to heat or radiation are independent of either topoisomerase activity or DNA polymerase function, but suggest that topoisomerases may have a regulatory role during the signaling of these mechanisms. The results of our experiments imply that maintenance of correct DNA topology prevents induction of the heat-shock response, and that heat-shock induction of a component of the full radiation resistance in yeast may be the consequence of topoisomerase I inactivation.
Boreham
D R
DR
Atomic Energy of Canada Limited, Radiation Biology Branch, Chalk River, Ontario.
Trivedi
A
A
Weinberger
P
P
Mitchel
R E
RE
D000222:Adaptation, Physiological; D002470:Cell Survival / Q000528:radiation effects; D004256:DNA Polymerase I / Q000502:physiology*; D004264:DNA Topoisomerases, Type I / Q000502:physiology*; D004250:DNA Topoisomerases, Type II / Q000502:physiology*; D006358:Hot Temperature; D011836:Radiation Tolerance / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000528:radiation effects
D004256:DNA Polymerase I; D004264:DNA Topoisomerases, Type I; D004250:DNA Topoisomerases, Type II