title: Saccharomyces cerevisiae homoserine kinase is homologous to prokaryotic homoserine kinases.
abstract: The Saccharomyces cerevisiae gene (THR1) encoding homoserine kinase (HK; EC 2.7.1.39) was cloned by complementation in yeast. Disruption of the THR1 gene results in threonine auxotrophy in yeast. Comparison of the amino acid sequences of yeast and bacterial HKs reveals substantial similarity.
authors.affiliation: Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
authors.forename: N P
authors.initials: NP
authors.lastname: Schultes
authors.forename: A D
authors.initials: AD
authors.lastname: Ellington
authors.forename: J M
authors.initials: JM
authors.lastname: Cherry
authors.forename: J W
authors.initials: JW
authors.lastname: Szostak
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D010770:Phosphotransferases / Q000235:genetics* / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor); D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D012689:Sequence Homology, Nucleic Acid; D013912:Threonine / Q000378:metabolism
chemical_list: D013912:Threonine; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C021370:homoserine kinase