title: Existence and unique N-terminal sequence of alpha II (omega) interferon in natural leukocyte interferon preparation.
abstract: Human alpha interferon produced in Sendai virus-stimulated leukocytes was purified by immunosorbent affinity chromatography and subjected to subtype analysis. All subtypes in leukocyte culture supernatant were confirmed in purified preparation. One of these subtypes was reactive in Western blotting with antibody specific to alpha II (omega). N-terminal amino acid sequence analysis of this particular subtype showed addition of two amino acids to the N-terminus of alpha II predicted through cDNA studies. The apparent cleavage site for leader sequence of alpha II was different from the site common to all other alpha subtypes. This is the first report on the isolation of natural alpha II and its unique N-terminal amino acid sequence.
authors.affiliation: Biotechnology Research Laboratories, JCR Pharmaceuticals Co., Ltd., Kobe, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Shirono
authors.forename: K
authors.initials: K
authors.lastname: Kono
authors.forename: J
authors.initials: J
authors.lastname: Koga
authors.forename: S
authors.initials: S
authors.lastname: Hayashi
authors.forename: A
authors.initials: A
authors.lastname: Matsuo
authors.forename: H
authors.initials: H
authors.lastname: Hiratani
mesh_terms: D000595:Amino Acid Sequence; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007370:Interferon Type I / Q000235:genetics / Q000302:isolation & purification*; D007962:Leukocytes / Q000032:analysis; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010222:Parainfluenza Virus 1, Human
chemical_list: D007370:Interferon Type I