Transforming growth-factor-beta (TGF-beta) inhibits albumin synthesis in normal human hepatocytes and in hepatoma HepG2 cells.
We explored the effect of transforming growth factor beta (TGF-beta), a cytokine that appears to play a central role in inflammatory events, on albumin expression by normal adult human hepatocytes and hepatoma cells. Addition of TGF-beta to primary human hepatocyte cultures resulted in a dramatic decrease in albumin accumulation and synthesis. This effect was dose-dependent, took place after a 48h incubation period and was maintained over 96h. TGF-beta-decreased albumin protein levels were associated with reduced albumin mRNA content. Actin mRNA levels were concomittantly increased. Comparable qualitative effects of TGF-beta were observed on human hepatoma HepG2 cells.
Busso
N
N
Laboratoires GLAXO, Centre de Recherches, Les Ulis, France.
Chesne
C
C
Delers
F
F
Morel
F
F
Guillouzo
A
A
D000328:Adult; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D002478:Cells, Cultured; D015342:DNA Probes; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008113:Liver Neoplasms; D008297:Male; D008970:Molecular Weight; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology; D012709:Serum Albumin / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D014158:Transcription, Genetic; D015291:Transforming Growth Factors / Q000494:pharmacology*
D015342:DNA Probes; D012333:RNA, Messenger; D011994:Recombinant Proteins; D012709:Serum Albumin; D015291:Transforming Growth Factors