A 69-base-pair monkey DNA sequence enhances simian virus 40 replication and transcription through multiple motifs.
We have determined that a 69-base-pair (bp) monkey DNA sequence, previously found to enhance simian virus 40 replication, has transcriptional enhancer activity as well. Consensus recognition sites for the transcription factor AP-1, present at each end of this sequence, are partially responsible for its replication- and transcription-enhancing activities. Other motifs within the 69-bp monkey sequence also act to increase the levels of replication and transcription. The activity of the monkey sequence is augmented by the presence of a simian virus 40 21-bp repeat. The 69-bp sequence enhances transcription but not replication from a distance. We conclude that the stimulation of replication and transcription can be uncoupled, suggesting that different mechanisms may be involved.
Szymanski
P
P
Department of Molecular and Cellular Biology, Roswell Park Memorial Institute, Buffalo, New York 14263.
Woodworth
M
M
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004742:Enhancer Elements, Genetic; D005796:Genes; D005814:Genes, Viral; D000882:Haplorhini; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D013539:Simian virus 40 / Q000235:genetics* / Q000502:physiology; D014158:Transcription, Genetic; D014779:Virus Replication