title: Regulation of trehalose metabolism in Saccharomyces cerevisiae mutants during temperature shifts.
abstract: Temperature shifts from 23 degrees C to 36 degrees C resulted in trehalose accumulation in Saccharomyces independently of genetic lesions in the cAMP-protein kinase cascade. In parallel, trehalose 6-phosphate synthase activity increased about 3-fold in all strains; the increase could be inhibited by cycloheximide, suggesting that protein synthesis was required. Heat shock treatment after the temperature shift led to a drastic increase in trehalose activity, and deactivation of the biosynthetic enzyme with a consequent drop in trehalose. Up to now no definite correlation between acquisition of thermotolerance and trehalose accumulation has been made.
authors.affiliation: Departamento de Bioquîmica, Universidade Federal do Rio de Janeiro, São Paulo, Brazil.
authors.forename: A C
authors.initials: AC
authors.lastname: Panek
authors.forename: J J
authors.initials: JJ
authors.lastname: Vânia
authors.forename: M F
authors.initials: MF
authors.lastname: Paschoalin
authors.forename: D
authors.initials: D
authors.lastname: Panek
mesh_terms: D000242:Cyclic AMP / Q000494:pharmacology; D004187:Disaccharides / Q000378:metabolism*; D009154:Mutation; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D013696:Temperature; D014199:Trehalose / Q000235:genetics / Q000378:metabolism*
chemical_list: D004187:Disaccharides; D014199:Trehalose; D000242:Cyclic AMP; D011494:Protein Kinases