Type-specific human papillomavirus detection in formalin-fixed, paraffin-embedded tissue sections using nonradioactive deoxyribonucleic acid probes.
DNA probes directly conjugated to horseradish peroxidase have been used successfully to detect human papillomavirus (HPV) types 6/11, 16, and 18 in formalin-fixed, paraffin-embedded tissue sections. By using silver enhancement of a heavy metal-modified diaminobenzidine precipitate, the sensitivity of human papillomavirus detection was significantly increased without compromising specificity. In studies comparing the specificity of the horseradish peroxidase-labeled probe/silver enhancement system to that of a biotinylated-DNA probe/streptavidin-alkaline phosphatase system, the former was found to be superior.
Higgs
T W
TW
Digene Diagnostics, Inc., Silver Spring, Maryland.
Moore
N J
NJ
Badawi
D Y
DY
Taub
F E
FE
D015100:3,3'-Diaminobenzidine; D015344:DNA Probes, HPV; D006652:Histological Techniques; D006735:Horseradish Peroxidase; D006801:Humans; D027383:Papillomaviridae / Q000302:isolation & purification*
D015344:DNA Probes, HPV; D015100:3,3'-Diaminobenzidine; D006735:Horseradish Peroxidase