Binding properties of avian retroviral proteins. III. Binding of protein ASLV MA(p19) to viral RNA and proviral DNA.
The binding of the avian retroviral matrix protein ASLV MA(p19) to homologous viral ssRNA and proviral dsDNA was analysed using electron microscopic methods combined with a special computer evaluation. No binding affinity of MA(p19) to homologous nondenatured or denatured viral RNA was found. In contrast, ASLV MA(p19) was shown to have one specific binding site on MAV-1 proviral dsDNA at position 6795 +/- 345 bp from the 5' end of the molecule. A second specific binding site was found in a cellular sequence.
Felsberg
J
J
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
Korb
J
J
Trávnícek
M
M
Ríman
J
J
D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics*; D009189:Avian Myeloblastosis Virus / Q000235:genetics*; D001665:Binding Sites; D002645:Chickens; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000378:metabolism* / Q000648:ultrastructure; D004268:DNA-Binding Proteins / Q000378:metabolism; D011533:Proviruses / Q000235:genetics / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism* / Q000648:ultrastructure; D012191:Retroviridae Proteins / Q000378:metabolism* / Q000648:ultrastructure; D014763:Viral Matrix Proteins / Q000378:metabolism* / Q000648:ultrastructure
D004279:DNA, Viral; D004268:DNA-Binding Proteins; D012367:RNA, Viral; D012191:Retroviridae Proteins; D014763:Viral Matrix Proteins; C040520:avian retrovirus proteins