title: The sodium pump needs its beta subunit.
abstract: The sodium pump Na,K-ATPase, located in the plasma membrane of all animal cells, is a member of a family of ion-translocating ATPases that share highly homologous catalytic subunits. In this family, only Na,K-ATPase has been established to be a heterodimer of catalytic (alpha) and glycoprotein (beta) subunits. The beta subunit has not been associated with the pump's transport or enzymatic activity, and its role in Na,K-ATPase function has been, until recently, a puzzle. In this review we describe what is known about the structure of beta and summarize evidence that expression of both alpha and beta subunits is required for Na,K-ATPase activity, that inhibition of glycosylation causes a decrease in accumulation of both alpha and beta subunits, and we provide evidence that pretranslational up-regulation of beta alone can lead to increased abundance of sodium pumps. These findings are all consistent with the hypothesis that the beta subunit regulates, through assembly of alpha beta heterodimers, the number of sodium pumps transported to the plasma membrane.
authors.affiliation: Department of Physiology and Biophysics, University of Southern California School of Medicine, Los Angeles 90033.
authors.forename: A A
authors.initials: AA
authors.lastname: McDonough
authors.forename: K
authors.initials: K
authors.lastname: Geering
authors.forename: R A
authors.initials: RA
authors.lastname: Farley
mesh_terms: D000818:Animals; D001692:Biological Transport; D015152:Blotting, Northern; D015153:Blotting, Western; D003001:Cloning, Molecular; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins / Q000502:physiology / Q000648:ultrastructure; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational; D012441:Saccharomyces cerevisiae / Q000235:genetics; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013552:Swine
chemical_list: D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; D000254:Sodium-Potassium-Exchanging ATPase