title: Tn10-derived transposons active in Bacillus subtilis.
abstract: Small derivatives of the Escherichia coli transposon Tn10, comprising IS10 ends and a chloramphenicol resistance gene, were introduced in Bacillus subtilis on a thermosensitive plasmid, pE194. In the presence of the Tn10 transposase gene fused to signals functional in B. subtilis, these derivatives transposed with a frequency of 10(-6) per element per generation. They had no highly preferred insertion site or region, as judged by restriction analysis of the chromosomal DNA, and generated auxotrophic and sporulation-deficient mutants with a frequency of about 1%. These results suggest that Tn10 derivatives might be a useful genetic tool in B. subtilis and possibly other gram-positive microorganisms.
authors.affiliation: Laboratoire de Génétique Microbienne, Institut National de Recherche Agronomique--Domaine de Vilvert, Jouy en Josas, France.
authors.forename: M A
authors.initials: MA
authors.lastname: Petit
authors.forename: C
authors.initials: C
authors.lastname: Bruand
authors.forename: L
authors.initials: L
authors.lastname: Jannière
authors.forename: S D
authors.initials: SD
authors.lastname: Ehrlich
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids; D011995:Recombination, Genetic; D015183:Restriction Mapping
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial