Adenosine alters the vascular effects of a diacylglycerol analogue and polymyxin B.
Endothelium-denuded rat aorta rings were used to study the possible relationship between protein kinase C and the mechanism of adenosine-induced smooth muscle relaxation. Adenosine (5 x 10(-4) M) partially relaxed the aortic rings contracted by either a depolarising amount of KCl (4 x 10(-2) M) or activation of protein kinase C with 1-oleoyl-2-acetyl-sn-glycerol (10(-6) M). The same amount of adenosine blocked the further relaxation obtained in the presence of polymyxin B (5 x 10(-5) M), a protein kinase C blocking agent. These results suggest a possible interaction in vascular smooth muscle between adenosine and protein kinase C.
Serban
D N
DN
Institute of Medicine and Pharmacy, Department of Physiology, Iaşi, Romania.
Macovei
C G
CG
Brănişteanu
D D
DD
Brănişteanu
D D
DD
D000241:Adenosine / Q000494:pharmacology*; D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects; D004075:Diglycerides / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects; D005989:Glycerides / Q000494:pharmacology*; D066298:In Vitro Techniques; D009045:Motor Endplate / Q000187:drug effects; D009119:Muscle Contraction / Q000187:drug effects; D009126:Muscle Relaxation / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D010660:Phenylisopropyladenosine / Q000494:pharmacology; D011112:Polymyxin B / Q000494:pharmacology*; D011113:Polymyxins / Q000494:pharmacology*; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats
D004075:Diglycerides; D005989:Glycerides; D011113:Polymyxins; D010660:Phenylisopropyladenosine; D011189:Potassium Chloride; C038237:1-oleoyl-2-acetylglycerol; D011112:Polymyxin B; D000241:Adenosine