title: Kinetic analysis of single sodium channels from canine cardiac Purkinje cells.
abstract: Single sodium channel events were recorded from cell-attached patches on single canine cardiac Purkinje cells at 10-13 degrees C. Data from four patches containing two to four channels and one patch with one channel were selected for quantitative analysis. The channels showed prominent reopening behavior at voltages near threshold, and the number of reopenings declined steeply with depolarization. Mean channel open time was a biphasic function of voltage with the maximum value (1-1.5 ms) occurring between -50 and -40 mV and lower values at more and at less hyperpolarized levels. Inactivation without opening was also prominent near threshold, and this occurrence also declined with depolarization. The waiting time distributions and the probability of being open showed voltage and time dependence as expected from whole-cell current studies. The results were analyzed in terms of a five-state Markovian kinetic model using both histogram analysis and a maximum likelihood method to estimate kinetic parameters. The kinetic parameters of the model fits were similar to those of GH3 pituitary cells (Horn, R., and C. A. Vandenberg. 1984. Journal of General Physiology. 84:505-534) and N1E115 neuroblastoma cells (Aldrich, R. W., and C. F. Stevens. Journal of Neuroscience. 7:418-431). Both histogram and maximum likelihood analysis implied that much of the voltage dependence of cardiac Na current is in its activation behavior, with inactivation showing modest voltage dependence.
authors.affiliation: Department of Medicine, University of Chicago, Illinois 60637.
authors.forename: B E
authors.initials: BE
authors.lastname: Scanley
authors.forename: D A
authors.initials: DA
authors.lastname: Hanck
authors.forename: T
authors.initials: T
authors.lastname: Chay
authors.forename: H A
authors.initials: HA
authors.lastname: Fozzard
mesh_terms: D000818:Animals; D004285:Dogs; D004594:Electrophysiology; D005544:Forecasting; D007700:Kinetics; D008954:Models, Biological; D011689:Purkinje Cells / Q000378:metabolism*; D015222:Sodium Channels / Q000378:metabolism* / Q000502:physiology; D013997:Time Factors
chemical_list: D015222:Sodium Channels