title: Synthesis of mitochondrial uncoupling protein in brown adipocytes differentiated in cell culture.
abstract: In order to characterize the biogenesis of unique thermogenic mitochondria of brown adipose tissue, differentiation of precursor cells isolated from mouse brown adipose tissue was studied in cell culture. Synthesis of mitochondrial uncoupling protein (UCP), F1-ATPase, and cytochrome oxidase was examined by L-[35S]methionine labeling and immunoblotting. For the first time, synthesis of physiological amounts of the UCP, a key and tissue-specific component of thermogenic mitochondria, was observed in cultures at about confluence (day 6), indicating that a complete differentiation of brown adipocytes was achieved in vitro. In postconfluent cells (day 8) the content of UCP decreased rapidly, in contrast to some other mitochondrial proteins (beta subunit of F1-ATPase, cytochrome oxidase). In these cells, it was possible, by using norepinephrine, to induce specifically the synthesis of the UCP but not of F1-ATPase or cytochrome oxidase. The maximal response was observed at 0.1 microM norepinephrine and the synthesis of UCP remained activated for at least 24 h. Detailed analysis revealed a major role of the beta-adrenergic receptors and elevated intracellular concentration of cAMP in stimulation of UCP synthesis. A quantitative recovery of the newly synthesized UCP in the mitochondrial fraction indicated completed biogenesis of functionally competent thermogenic mitochondria.
authors.affiliation: Institute of Physiology, Czechoslovak Academy of Sciences, Prague.
authors.forename: J
authors.initials: J
authors.lastname: Kopecký
authors.forename: M
authors.initials: M
authors.lastname: Baudysová
authors.forename: F
authors.initials: F
authors.lastname: Zanotti
authors.forename: D
authors.initials: D
authors.lastname: Janíková
authors.forename: S
authors.initials: S
authors.lastname: Pavelka
authors.forename: J
authors.initials: J
authors.lastname: Houstĕk
mesh_terms: D002001:Adipose Tissue, Brown / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins; D002454:Cell Differentiation; D002478:Cells, Cultured; D003576:Electron Transport Complex IV / Q000096:biosynthesis / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional; D007473:Ion Channels; D007700:Kinetics; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D008715:Methionine / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins; D006180:Proton-Translocating ATPases / Q000096:biosynthesis / Q000302:isolation & purification; D013462:Sulfur Radioisotopes; D000071256:Uncoupling Protein 1
chemical_list: D002352:Carrier Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D013462:Sulfur Radioisotopes; D000071256:Uncoupling Protein 1; D008715:Methionine; D003576:Electron Transport Complex IV; D006180:Proton-Translocating ATPases