Identification by anti-idiotype antibodies of an intracellular membrane protein that recognizes a mammalian endoplasmic reticulum retention signal.
Monoclonal antibodies were raised against antibodies to distinct carboxy-terminal KDEL sequences of two soluble, resident endoplasmic reticulum proteins. These anti-idiotype reagents recognize an intrinsic membrane protein with characteristics expected of a receptor responsible for the recognition and return of resident proteins to the endoplasmic reticulum.
Vaux
D
D
Cell Biology Programm, European Molecular Biology Laboratory, Heidelberg, FRG.
Tooze
J
J
Fuller
S
S
D000595:Amino Acid Sequence; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D020126:Brefeldin A; D002451:Cell Compartmentation / Q000187:drug effects; D003517:Cyclopentanes / Q000494:pharmacology; D004721:Endoplasmic Reticulum / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D006825:Hybridomas / Q000502:physiology; D008565:Membrane Proteins / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009842:Oligopeptides / Q000276:immunology; D011506:Proteins / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000276:immunology / Q000502:physiology*; D018000:Receptors, Peptide; D012995:Solubility
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D003517:Cyclopentanes; C064459:KDEL receptor; D008565:Membrane Proteins; D009842:Oligopeptides; D011506:Proteins; D011956:Receptors, Cell Surface; D018000:Receptors, Peptide; D020126:Brefeldin A