Coexpression of biologically active simian immunodeficiency virus (SIV) Rev and Env in an SV40 system: the SIV rev gene regulates env expression.
The coexpression of biologically active simian immunodeficiency virus (SIV) Rev and Env gene products was obtained in COS-1 cells from a single SIV subgenomic segment (which contains both exons of rev and the entire env gene) cloned into a SV40-directed vector. The SIVsm Rev trans-activated the expression of the full-length env mRNA and was required for the production of envelope glycoproteins. Furthermore, the alignment of the structural conservation of the Rev functional domains among all HIV and SIV was analyzed.
Cheng
S M
SM
Biotechnology and Microbiology Division, Wyeth-Ayerst Research, Radnor, Pennsylvania 19087.
Blume
M
M
Lee
S G
SG
Hung
P P
PP
Hirsch
V M
VM
Johnson
P R
PR
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002872:Chromosome Deletion; D005091:Exons; D015967:Gene Expression Regulation, Viral; D015694:Gene Products, rev / Q000235:genetics; D005814:Genes, Viral; D015752:Genes, env; D015757:Genes, rev; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D015302:Simian Immunodeficiency Virus / Q000235:genetics*; D013539:Simian virus 40 / Q000235:genetics*; D012984:Software; D014759:Viral Envelope Proteins / Q000235:genetics*
D015694:Gene Products, rev; D012333:RNA, Messenger; D014759:Viral Envelope Proteins