A novel function of the transforming domain of E1a: repression of AP-1 activity.
Adenovirus E1a represses transcription of the collagenase gene via the phorbol ester-responsive element (collTRE). The mechanism involves inhibition of the trans-activating function of the transcription factor AP-1 without reduction of its synthesis and without any apparent change in DNA binding or composition. The ability of E1a to downmodulate AP-1 is a unique property among dominant oncogenes. This repression depends on conserved region 1, one of the transforming domains of E1a, indicating that it is an integral feature of adenovirus transformation.
Offringa
R
R
Sylvius Laboratories, Laboratory for Molecular Carcinogenesis, University of Leiden, The Netherlands.
Gebel
S
S
van Dam
H
H
Timmers
M
M
Smits
A
A
Zwart
R
R
Stein
B
B
Bos
J L
JL
van der Eb
A
A
Herrlich
P
P
D017456:Adenovirus Early Proteins; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004794:Enzyme Repression; D015971:Gene Expression Regulation, Enzymologic; D006367:HeLa Cells / Q000201:enzymology; D006801:Humans; D003012:Microbial Collagenase / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun; D011965:Receptors, Glucocorticoid / Q000235:genetics; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection
D017456:Adenovirus Early Proteins; D004268:DNA-Binding Proteins; D009856:Oncogene Proteins, Viral; D016755:Proto-Oncogene Proteins c-jun; D011965:Receptors, Glucocorticoid; D014157:Transcription Factors; D011505:Protein-Tyrosine Kinases; D003012:Microbial Collagenase