title: CD8 lymphocyte subpopulations in Peyer's patches induced by reovirus serotype 1 infection.
abstract: CD8 lymphocyte population heterogeneity has been examined by using reovirus serotype 1, strain Lang (reovirus 1/L) as a model infection. We have previously reported that gut mucosal infection with reovirus stimulates the appearance of virus-specific cytotoxic T cell precursors (pCTL) in Peyer's patches (PP). The effectors that mediate reovirus-specific cytotoxicity were found to express both the Thy-1 and CD8 Ag and were MHC-restricted in their recognition of reovirus Ag. To further characterize the virus-specific precursor and effector cells we have analyzed PP cells for the expression of a novel surface Ag (termed germinal center and T cell Ag (GCT)) found on germinal center B cells and a subpopulation of CD8+ T cells. Gut mucosal infection with reovirus 1/L is capable of increasing the proportion of GCT+ CD8+ T cells in PP. Positive selection as well as depletion of GCT+ cells has demonstrated that pCTL can express this Ag, and depletion experiments have demonstrated that effector CTL express the GCT Ag. Thus, a subpopulation of GCT+ cells have been identified as Ag-specific precursor and effector CTL. These observations indicate that the expression of the GCT Ag may provide a means to identify recently stimulated pCTL or effector CTL in gut mucosal tissues.
authors.affiliation: Department of Biology, University of Pennsylvania, Philadelphia.
authors.forename: S D
authors.initials: SD
authors.lastname: London
authors.forename: J A
authors.initials: JA
authors.lastname: Cebra-Thomas
authors.forename: D H
authors.initials: DH
authors.lastname: Rubin
authors.forename: J J
authors.initials: JJ
authors.lastname: Cebra
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D000950:Antigens, Ly / Q000032:analysis; D016827:CD8 Antigens; D003602:Cytotoxicity, Immunologic; D004386:Duodenum / Q000276:immunology; D005434:Flow Cytometry; D007111:Immunity, Cellular; D051379:Mice; D008809:Mice, Inbred C3H; D010581:Peyer's Patches / Q000166:cytology / Q000276:immunology*; D012088:Reoviridae Infections / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D000950:Antigens, Ly; D016827:CD8 Antigens