Molecular cloning and sequence analysis of the fusion glycoprotein gene of human parainfluenza virus type 2.
A cDNA clone containing a 2.0-kb insert was identified as the human parainfluenza virus type 2 (PI2) fusion glycoprotein gene by hybridizing with a viral RNA probe and a synthetic oligonucleotide derived from a conserved sequence found in other paramyxovirus fusion protein genes. The complete nucleotide sequence of the glycoprotein gene was determined by the dideoxynucleotide sequencing procedure and found to contain a single, large open reading frame encoding a protein of 551 amino acids with a calculated molecular weight of 59,664. Comparison of the P12 fusion protein with those of other paramyxoviruses indicated similarities in overall length, N-terminal signal peptide sequence (amino acids 7 to 25), C-terminal membrane-spanning region (amino acids 486 to 513), and a highly conserved fusion sequence region at the N-terminus of the F1 subunit (amino acids 107 to 132).
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Department of Microbiology, University of Alabama, Birmingham 35294.
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Matsuoka
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D000595:Amino Acid Sequence; D001483:Base Sequence; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010223:Parainfluenza Virus 2, Human / Q000235:genetics*; D014760:Viral Fusion Proteins / Q000235:genetics*
D015345:Oligonucleotide Probes; D014760:Viral Fusion Proteins