Fosfadecin and fosfocytocin, new nucleotide antibiotics produced by bacteria.
Two new nucleotide antibiotics, fosfadecin and fosfocytocin, have been isolated from the culture filtrates of Pseudomonas viridiflava PK-5 and Pseudomonas fluorescens PK-52, respectively. These antibiotics were purified by column chromatographies using adsorption, gel filtration and ion exchange resins. On the basis of the spectroscopic and degradation studies, the chemical structures of fosfadecin and fosfocytocin were determined. These antibiotics were either enzymatically or chemically hydrolyzed to generate fosfomycin and a new antibiotic, fosfoxacin, which are also produced in the culture filtrates. They showed antibacterial activity against Gram-positive and Gram-negative bacteria. The antibacterial activity of these nucleotide antibiotics was weaker than that of fosfomycin and fosfoxacin.
Katayama
N
N
Microbiology Research Laboratories, Takeda Chemical Industries, Ltd., Osaka, Japan.
Tsubotani
S
S
Nozaki
Y
Y
Harada
S
S
Ono
H
H
D000244:Adenosine Diphosphate / Q000031:analogs & derivatives / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology / Q000627:therapeutic use; D001419:Bacteria / Q000187:drug effects*; D002845:Chromatography; D003565:Cytidine Diphosphate / Q000031:analogs & derivatives / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology; D003568:Cytidine Monophosphate / Q000031:analogs & derivatives / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology; D004927:Escherichia coli Infections / Q000188:drug therapy; D005285:Fermentation; D005578:Fosfomycin / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D015394:Molecular Structure; D011549:Pseudomonas / Q000145:classification / Q000378:metabolism*; D013203:Staphylococcal Infections / Q000188:drug therapy
D000900:Anti-Bacterial Agents; C063720:fosfadecin; C063722:fosfoxacin; C063721:fosfocytocin; D005578:Fosfomycin; D000244:Adenosine Diphosphate; D003565:Cytidine Diphosphate; D003568:Cytidine Monophosphate