Estrogens, androgens, and progestins in follicular fluid from preovulatory follicles: identification and quantification by gas chromatography/mass spectrometry associated with stable isotope dilution.
The synthesis and use of stable isotope-labeled analogs of various steroids have made it possible to undertake a study of follicular fluid (FF) aspirated from mature and preovulatory follicles. Our previous results have been brought together here in order to review quantitative work done by gas chromatography-mass spectrometry. A positive gradient between peripheral plasma and FF concentrations of a steroid suggests the possibility of ovarian biosynthesis. This is particularly relevant to the catecholestrogens, 19-norsteroids, and some corticosteroids.
Dehennin
L
L
Fondation de Recherche en Hormonologie, Fresnes, France.
D000728:Androgens / Q000032:analysis*; D002393:Estrogens, Catechol / Q000032:analysis*; D005260:Female; D015571:Follicular Fluid / Q000032:analysis*; D005498:Follicular Phase / Q000502:physiology*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007201:Indicator Dilution Techniques; D007553:Isotope Labeling; D009654:Norsteroids / Q000032:analysis; D011372:Progestins / Q000032:analysis*
D000728:Androgens; D002393:Estrogens, Catechol; D009654:Norsteroids; D011372:Progestins