An evaluation of a solid phase red cell adherence test for detecting platelet-associated IgG in immune thrombocytopenia.
A solid phase red cell adherence (SPRCA) test, designed for platelet cross-match testing, was evaluated to determine its efficacy in detecting platelet-associated IgG (PAIgG) in immune thrombocytopenic purpura (ITP). This new method was compared to the platelet suspension immunofluorescence (PSIF) test. Fifty-three patient samples were tested by both methods, including 17 for whom a clinical diagnosis of ITP had been made. The SPRCA method showed 65% sensitivity, whereas the PSIF method showed 53% sensitivity. The specificity for both was 100%. The SPRCA compared favorably to the PSIF test in sensitivity and cost effectiveness. The SPRCA test is rapid, easy to perform, and suitable for detecting PAIgG in ITP.
Jones
C D
CD
Department of Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, California 90048.
Gould
L M
LM
Lee
S
S
D001792:Blood Platelets / Q000032:analysis* / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology*; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D006801:Humans; D007104:Immune Adherence Reaction; D007074:Immunoglobulin G / Q000032:analysis*; D011696:Purpura, Thrombocytopenic / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D011933:Reagent Kits, Diagnostic
D007074:Immunoglobulin G; D011933:Reagent Kits, Diagnostic