cAMP dependent chloride conductance is not different in cystic fibrosis fibroblasts.
A chloride conductive pathway has been demonstrated in human skin fibroblasts and a defective cAMP dependent activation of this conductance in Cystic Fibrosis (CF) fibroblasts has been also reported. Chloride transport by the same reported method was studied in normal and CF skin fibroblasts. The stimulation of this pathway was not obtained consistently by the addition of dibutyryl cAMP. The addition of prostaglandin E1 (PGE1) increased the intracellular [cAMP] but did not increase the conductivity of the pathway consistently. Neither the basal nor the dibutyryl cAMP or the PGE1 stimulated chloride conductance differed significantly in CF fibroblasts.
Squassoni
E
E
Cystic Fibrosis Center, Ospedale Borgo Trento, Verona, Italy.
Cabrini
G
G
Berton
G
G
D000527:Alprostadil / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D002712:Chlorides / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism*; D003550:Cystic Fibrosis / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D012867:Skin / Q000166:cytology; D013806:Theophylline / Q000494:pharmacology
D002712:Chlorides; D003994:Bucladesine; D013806:Theophylline; D000242:Cyclic AMP; D000527:Alprostadil