title: Immunohistologic study of mononuclear cell infiltrates in oral squamous cell carcinomas.
abstract: Intensity of mononuclear cell infiltrates in 50 oral cancer tissues was evaluated by immunohistochemical stainings. The intensity of the infiltrates was graded into four degrees from marked ( ) to absent (-); and the degree of each infiltrated lymphocyte subpopulation including Leu-3a3b/Leu-2a ratio was matched against clinical and histopathologic features. The vast majority of the infiltrated lymphocytes had antigens which were reactive with anti-T cell antibodies. In T cell subsets, Leu-3a3b+ cells infiltrated clearly (grade ++ or ) in 44 cases, and infiltration greater than grade ++ of Leu-2a+ cells was found in nearly half of the cases (26 of 50). In contrast, B cells (Leu-14+ cells) were obviously detectable in only a few cases, and moderate or marked infiltration of the natural killer (NK) population (Leu-11b+ cells) was not observed. Regarding correlation of T cell infiltrates with clinicopathologic features of the tumors, the degrees of Leu-4+ cell and Leu-2a+ cell infiltration became weak with advancing T stage. Leu-2a+ cells also infiltrated weakly into diffusely invading tumors. Out of 28 cases with no metastasis, 22 cases (78.6%) showed grade ++ approximately of Leu-2a+ cells, and only 4 (18.2%) of 22 cases with lymph node metastasis were clearly positive for Leu-2a+ cells (p less than 0.001). However, Leu-3a3b+ cell infiltration was only slightly related to T stage, metastasis, and to mode of invasion. Therefore, the Leu-3a3b/Leu-2a ratio increased significantly in advanced (T3 + T4) tumors, metastasized cases, or diffuse invasion type.
authors.affiliation: Department of Oral Surgery, Kochi Medical School, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Hirota
authors.forename: E
authors.initials: E
authors.lastname: Ueta
authors.forename: T
authors.initials: T
authors.lastname: Osaki
authors.forename: Y
authors.initials: Y
authors.lastname: Ogawa
mesh_terms: D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008207:Lymphatic Metastasis; D009061:Mouth Mucosa / Q000276:immunology / Q000473:pathology*; D009062:Mouth Neoplasms / Q000276:immunology / Q000473:pathology*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal