title: Expression cloning of a receptor for murine granulocyte colony-stimulating factor.
abstract: Two cDNAs encoding the receptor for murine granulocyte colony-stimulating factor (G-CSF) were isolated from a CDM8 expression library of mouse myeloid leukemia NFS-60 cells, and their nucleotide sequences were determined. Murine G-CSF receptor expressed in COS cells could bind G-CSF with an affinity and specificity similar to that of the native receptor expressed by mouse NFS-60 cells. The amino acid sequence encoded by the cDNAs has demonstrated that murine G-CSF receptor is an 812 amino acid polypeptide (Mr, 90,814) with a single transmembrane domain. The extracellular domain consists of 601 amino acids with a region of 220 amino acids that shows a remarkable similarity to rat prolactin receptor. The cytoplasmic domain of the G-CSF receptor shows a significant similarity with parts of the cytoplasmic domain of murine interleukin-4 receptor. A 3.7 kb mRNA coding for the G-CSF receptor could be detected in mouse myeloid leukemia NFS-60 and WEHI-3B D+ cells as well as in bone marrow cells.
authors.affiliation: Osaka Bioscience Institute, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Fukunaga
authors.forename: E
authors.initials: E
authors.lastname: Ishizaka-Ikeda
authors.forename: Y
authors.initials: Y
authors.lastname: Seto
authors.forename: S
authors.initials: S
authors.lastname: Nagata
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D003115:Colony-Stimulating Factors / Q000378:metabolism; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D015723:Gene Library; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D016188:Receptors, Granulocyte Colony-Stimulating Factor; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D015398:Signal Transduction; D014162:Transfection
chemical_list: D003115:Colony-Stimulating Factors; D011956:Receptors, Cell Surface; D016188:Receptors, Granulocyte Colony-Stimulating Factor; D004247:DNA