[Relative location of anthroyl derivatives of stearic acid in low density lipoproteins].
Effective quenching constants (K'sv) of 2-, 7- and 12-(9-anthroyloxy)stearic acid (n-AS) fluorescence in LDL were determined. Spin probes I(m, n) (n = 3, 7, 10, 14) and I- anions were used as quenchers. Quenching of 2-AS and 12-AS fluorescence by I(m, n) was the more effective, the deeper spin probe nitroxyl fragment was located (the greater n was); maximal K'sv value corresponded to I(1,14). By contrast, for 7-AS the quenching by I(12,3) was the most effective. 2-AS and 12-AS spectra maxima and fluorescence polarization were similar. We concluded that the 2-AS chromophore was located deeper in LDL phospholipid monolayer than chromophore of 7-AS (as was the case for 12-AS).
Valentinova
N V
NV
Bushueva
T L
TL
Medvedeva
N V
NV
Morozkin
A D
AD
D004578:Electron Spin Resonance Spectroscopy; D005456:Fluorescent Dyes; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013229:Stearic Acids / Q000097:blood / Q000737:chemistry*
D005456:Fluorescent Dyes; D008077:Lipoproteins, LDL; D013229:Stearic Acids; C026653:12-(9-anthroyloxy)stearic acid
Otnositel'noe raspolozhenie antroil'nykh proizvodnykh stearinovoĭ kisloty v lipoproteidakh nizkoĭ plotnosti.