Production of 21-deoxyaldosterone by rat adrenal tissue in vitro: evidence for an alternative biosynthetic pathway of aldosterone.
Quartered rat adrenal glands transformed labelled 21-deoxyaldosterone into aldosterone in vitro. 21-deoxyaldosterone was released from the quartered rat adrenals in vitro in amounts 10 times lower than those of aldosterone and 18-hydroxycorticosterone. The production of all three steroids was qualitatively dependent on the same regulatory elements (electrolytes, ACTH, exogenous precursors, zonal specificity). However, quantitative differences could be observed. The results support a role for 21-deoxyaldosterone as a facultative precursor of aldosterone and indicate that the regulation of its production shows similarities to that of aldosterone.
Lewicka
S
S
Department of Pharmacology, University of Heidelberg, FRG.
Bige
K
K
Weindel
K
K
Winter
J
J
Pies
W
W
Vecsei
P
P
D015069:18-Hydroxycorticosterone / Q000378:metabolism*; D000311:Adrenal Glands / Q000187:drug effects / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000450:Aldosterone / Q000031:analogs & derivatives* / Q000096:biosynthesis* / Q000502:physiology; D000818:Animals; D046508:Culture Techniques; D008297:Male; D011188:Potassium / Q000494:pharmacology; D011191:Potassium Deficiency / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000172:deficiency / Q000494:pharmacology; D015384:Zona Glomerulosa / Q000378:metabolism*
C032486:21-deoxyaldosterone; D000450:Aldosterone; D015069:18-Hydroxycorticosterone; D000324:Adrenocorticotropic Hormone; D012964:Sodium; D011188:Potassium