title: Sustained benefit of temporary limited reperfusion in skeletal muscle following ischemia.
abstract: Limiting the rate of reperfusion blood flow following prolonged ischemia in skeletal muscle has been shown beneficial. However, the persistence of this benefit with reinstitution of normal blood flow remains undefined. We investigated the role of temporary limited reperfusion on ischemia-reperfusion injury in an isolated gracilis muscle model in six anesthetized dogs. Both gracilis muscles were subjected to 6 hr of ischemia followed by 2 hr of reperfusion. Reperfusion blood flow was limited for the first hour in one gracilis muscle to its preischemic rate followed by a second hour of normal reperfusion (LR/NR). The contralateral muscle underwent 2 hr of normal reperfusion (NR/NR). Muscle injury was quantified by technetium-99m pyrophosphate (TcPyp) uptake and by histochemical staining using triphenyltetrazolium chloride (TTC) with planimetry of the infarct size. Capillary permeability was evaluated by muscle weight gain. Results are reported as the mean +/- SEM: [table: see text] These data demonstrate a sustained benefit from temporary limited reperfusion. This methodology should be considered in the surgical management of the acutely ischemic limb.
authors.affiliation: University of Medicine and Dentistry of New Jersey-New Jersey Medical School, Newark 07103-2757.
authors.forename: R J
authors.initials: RJ
authors.lastname: Anderson
authors.forename: R
authors.initials: R
authors.lastname: Cambria
authors.forename: J
authors.initials: J
authors.lastname: Kerr
authors.forename: R W
authors.initials: RW
authors.lastname: Hobson
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity; D001794:Blood Pressure; D011756:Diphosphates; D004285:Dogs; D005260:Female; D006651:Histocytochemistry; D007511:Ischemia / Q000473:pathology; D007700:Kinetics; D008297:Male; D009132:Muscles / Q000098:blood supply* / Q000473:pathology; D009929:Organ Size; D015427:Reperfusion Injury / Q000517:prevention & control*; D013667:Technetium; D016698:Technetium Tc 99m Pyrophosphate; D013778:Tetrazolium Salts; D013997:Time Factors
chemical_list: D011756:Diphosphates; D013778:Tetrazolium Salts; D016698:Technetium Tc 99m Pyrophosphate; D013667:Technetium; C009591:triphenyltetrazolium