Identification of transcription promoter regions from rat mtDNA that are utilized in vivo by the bacterial RNA polymerase.
We have used a prokaryotic promoter-identification vector, pKO-1, to isolate rat mitochondrial DNA (mtDNA) sequences that can act as bacterial transcription promoters. Three putative promoter-containing clones that hydridized to mtDNA probes were identified. The strength of the promoters was quantitated by measuring galactokinase activity. The three promoters mapped to three distinct regions of the mtDNA - one within the 5' half of the 16S rRNA gene, one within the ATPase subunit 6 gene, and the last at the carboxy terminal end of the cytochrome oxidase subunit I gene.
Staub
J M
JM
Department of Biochemistry, Eastern Virginia Medical School, Norfolk 23507.
Castora
F J
FJ
D000251:Adenosine Triphosphatases / Q000235:genetics; D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D016384:Consensus Sequence; D004272:DNA, Mitochondrial / Q000737:chemistry*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005260:Female; D005686:Galactokinase / Q000096:biosynthesis / Q000235:genetics; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012336:RNA, Ribosomal, 16S / Q000737:chemistry; D051381:Rats; D011919:Rats, Inbred Strains / Q000235:genetics*; D015183:Restriction Mapping; D014158:Transcription, Genetic
D004272:DNA, Mitochondrial; D012336:RNA, Ribosomal, 16S; D003576:Electron Transport Complex IV; D005686:Galactokinase; D012321:DNA-Directed RNA Polymerases; D000251:Adenosine Triphosphatases