Soluble cytochromes and ferredoxins from the marine purple phototrophic bacterium, Rhodopseudomonas marina.
Four soluble c-type cytochromes, the high redox potential 4-Fe-S ferredoxin known as HiPIP, a large molecular weight 2-Fe-S ferredoxin and a 4-Fe-S 'bacterial' ferredoxin, were isolated from extracts of two strains of Rps. marina. Cytochrome c-550, cytochrome c' and cytochrome c-549 were previously described, and we have extended their characterization. Cytochrome c-558, which has not previously been observed in Rps. marina, appears to be a low-spin isozyme of the more commonly observed high-spin cytochrome c'. HiPIP, which was not observed in previous work, was found to be abundant in Rps. marina. The 2-Fe-S ferredoxin, which has previously been observed only in Rps. palustris, has a native size greater than 100 kDa and a subunit size of 17 kDa. The 'bacterial' ferredoxin appears to have only a single four-iron-sulfur cluster. We examined photosynthetic membranes by difference spectroscopy and found abundant c-type cytochromes. Approximately one-quarter of the heme can be reduced by ascorbate and the remainder by dithionite. There is 2 nm difference between the high-potential heme (554 nm) and the low (552 nm). These characteristics resemble those of the tetraheme reaction center cytochrome of Rps. viridis. In addition to the electron transfer components, we found small amounts of a fluorescent yellow protein which has spectral resemblance to a photoactive yellow protein from Ec. halophila.
Meyer
T E
TE
Department of Biochemistry, University of Arizona, Tucson 85721.
Cannac
V
V
Fitch
J
J
Bartsch
R G
RG
Tollin
D
D
Tollin
G
G
Cusanovich
M A
MA
D001426:Bacterial Proteins / Q000302:isolation & purification*; D002845:Chromatography; D003574:Cytochrome c Group / Q000302:isolation & purification; D003580:Cytochromes / Q000302:isolation & purification*; D005288:Ferredoxins / Q000302:isolation & purification*; D007506:Iron-Sulfur Proteins / Q000302:isolation & purification; D008164:Luminescent Proteins / Q000302:isolation & purification; D045322:Photosynthetic Reaction Center Complex Proteins; D012241:Rhodopseudomonas / Q000032:analysis*; D013053:Spectrophotometry
D001426:Bacterial Proteins; D003574:Cytochrome c Group; D003580:Cytochromes; D005288:Ferredoxins; D007506:Iron-Sulfur Proteins; D008164:Luminescent Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; C007943:high potential iron-sulfur protein; C054562:yellow fluorescent protein, Bacteria