[The use of a molecular probe containing a specific cDNA consisting of bacteriophage M13 for detecting the hepatitis A virus in clinical specimens].
A probe was constructed containing a fragment of DNA replica of hepatitis A virus (HAV) RNA within bacteriophage M13 single-stranded DNA which allowed 10(-12) g of viral RNA to be tested. Hybridization of 32P-labeled probe with total RNA from 559 samples of blood, saliva, and urine from patients with viral hepatitis A revealed the presence of HAV RNA in 14% of the samples. In the 1st week of the jaundice period HAV RNA was detected in 40% (15 positive samples out of the 39 tested), in the 2nd week 26% (14 out of 54). HAV RNA was demonstrated in 20 out of 236 blood samples from subjects in a focus of HA outbreak. Six out of 9 subjects subsequently developing the disease were detected 7-10 days before the onset of the clinical symptoms. The proposed method may be useful for detection of HAV carriers in the latent period for their isolation in order to prevent further development of epidemic.
Nikolaeva
E S
ES
Volchek
I A
IA
Naumov
V A
VA
Zelenin
S M
SM
Popova
V S
VS
Karginov
V A
VA
Mertvetsov
N P
NP
D001435:Bacteriophages / Q000235:genetics*; D002353:Carrier State / Q000175:diagnosis / Q000382:microbiology; D004247:DNA; D015342:DNA Probes; D004279:DNA, Viral; D006506:Hepatitis A / Q000175:diagnosis / Q000382:microbiology; D006507:Hepatovirus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000235:genetics; D012463:Saliva / Q000382:microbiology; D014556:Urine / Q000382:microbiology; D014766:Viremia / Q000175:diagnosis / Q000382:microbiology
D015342:DNA Probes; D004279:DNA, Viral; D012367:RNA, Viral; D004247:DNA
Ispol'zovanie molekuliarnogo zonda, soderzhashchego spetsificheskuiu kDNK v sostave bakteriofaga M13, dlia detektsii virusa gepatita A v klinicheskikh obraztsakh.