Detection of multiple forms of human ceruloplasmin. A novel Mr 200,000 form.
Three polypeptides with apparent Mr = 200,000, 135,000, and 115,000, reacting with antibody to human ceruloplasmin (Cp), were consistently found in sera of normal adult and newborn subjects, patients with Wilson's disease, as well as in the oxidase-active fraction of purified human Cp, resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The concentrations of the three Cp polypeptides were proportional to the total Cp oxidase activity measured in whole serum. Peptide mapping revealed that the three Cp polypeptides were closely related. Cross-linking of Cp135 resulted in dimers with electrophoretic mobility similar to that of Cp200. A common shift in electrophoretic mobility following N-glycanase treatment indicated that all three polypeptides were N-glycosylated, and that the apparent differences in molecular mass could not be related to the carbohydrate moiety. Immunoprecipitates of cell lysates of [35S]cysteine labeled HepG2 cells revealed the presence of two species of newly synthesized Cp polypeptides, Mr 200,000 and 135,000, which were secreted into the media. Secretion of Cp200 by the human liver appears to be physiologic and may be the result of posttranslational modification of Cp135.
Sato
M
M
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461.
Schilsky
M L
ML
Stockert
R J
RJ
Morell
A G
AG
Sternlieb
I
I
D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D002570:Ceruloplasmin / Q000096:biosynthesis / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006527:Hepatolenticular Degeneration / Q000097:blood; D006801:Humans; D007118:Immunoassay; D008113:Liver Neoplasms; D008970:Molecular Weight; D010446:Peptide Fragments / Q000302:isolation & purification; D010449:Peptide Mapping; D012016:Reference Values
D010446:Peptide Fragments; D002570:Ceruloplasmin