title: A general method for the transfer of plasmid-borne mutant alleles to the chromosome of Klebsiella pneumoniae using bacteriophage lambda: transfer of pqq genes.
abstract: A generally applicable method is described for reintroduction of mutant plasmid-borne alleles to the chromosome of Klebsiella pneumoniae using bacteriophage lambda. We used this method to make stable chromosomal transposon insertions in genes for biosynthesis of pyrroloquinoline quinone in K. pneumoniae.
authors.affiliation: E.C. Slater Institute for Biochemical Research, University of Amsterdam, The Netherlands.
authors.forename: J J
authors.initials: JJ
authors.lastname: Meulenberg
authors.forename: W A
authors.initials: WA
authors.lastname: Loenen
authors.forename: E
authors.initials: E
authors.lastname: Sellink
authors.forename: P W
authors.initials: PW
authors.lastname: Postma
mesh_terms: D000483:Alleles; D010582:Bacteriophage lambda / Q000235:genetics; D002876:Chromosomes, Bacterial / Q000378:metabolism*; D003067:Coenzymes / Q000378:metabolism; D004251:DNA Transposable Elements; D007711:Klebsiella pneumoniae / Q000235:genetics*; D009154:Mutation; D045542:PQQ Cofactor; D010957:Plasmids; D015363:Quinolones / Q000378:metabolism*; D015183:Restriction Mapping
chemical_list: D003067:Coenzymes; D004251:DNA Transposable Elements; D015363:Quinolones; D045542:PQQ Cofactor