Regulation of Na+,K(+)-ATPases. I. Cloning and analysis of the 5'-flanking region of the rat NKAA2 gene encoding the alpha 2 subunit.
The nucleotide (nt) sequence of the genomic clone, spanning at least the first two exons of the rat Na+,K(+)-ATPase alpha 2 subunit-encoding gene and 6.5 kb of the 5'-flanking region, has been determined. S1 nuclease mapping analysis of the 5' end of the Na+,K(+)-ATPase mRNA indicated that the transcription start point (tsp) is located 105 bp upstream from the start codon. The tsp was identical among three adult-rat tissues (brain, skeletal muscle and heart) which produce the alpha 2 isoform. A TATA-like sequence was found 33 bp upstream from the tsp. In addition, multiple consensus binding sites for a wide variety of regulatory proteins were present throughout the upstream and downstream tsp-flanking regions. A remarkable conservation in the nt sequence of the 5'-flanking region was confirmed between the rat and human genes.
Kawakami
K
K
Department of Biology, Jichi Medical School, Tochigi, Japan.
Yagawa
Y
Y
Nagano
K
K
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology; D003001:Cloning, Molecular; D005091:Exons; D015971:Gene Expression Regulation, Enzymologic; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D011401:Promoter Regions, Genetic; D051381:Rats; D016415:Sequence Alignment; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics* / Q000378:metabolism; D016385:TATA Box; D014158:Transcription, Genetic
D000254:Sodium-Potassium-Exchanging ATPase