Evaluation of a new commercial assay kit for quantification of lactate dehydrogenase isoenzyme 1 in serum.
A new method for measuring lactate dehydrogenase (EC 1.1.1.27) isoenzyme 1 using sodium perchlorate as a chaotropic chemical selective inhibitor of all lactate dehydrogenase isoenzymes containing M-subunit was evaluated. Results with this method were precise (between-day coefficient of variation less than 9%), highly linear (coefficient of correlation = 0.9998) and correlated well with lactate dehydrogenase isoenzyme 1 as determined by the conventional immunological method (coefficient of correlation = 0.976). The reference interval for 307 healthy subjects was estimated to be 23-46 U/l (95% central range, determined non-parametrically). Being simple, convenient and amenable to automation, the method provides a substantial saving in labour and reagent costs when compared with alternative analytical approaches.
Panteghini
M
M
1. Laboratorio Analisi Chimico-Cliniche, Spedali Civili, Brescia, Italy.
Bonora
R
R
Pagani
F
F
D005069:Evaluation Studies as Topic; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D010472:Perchlorates; D011233:Precipitin Tests / Q000379:methods; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D012015:Reference Standards; D012044:Regression Analysis; D013997:Time Factors
D007527:Isoenzymes; D010472:Perchlorates; D011933:Reagent Kits, Diagnostic; D007770:L-Lactate Dehydrogenase