title: A third essential DNA polymerase in S. cerevisiae.
abstract: DNA polymerases I and III are essential for viability of S. cerevisiae. We have cloned and analyzed POL2, the gene encoding the catalytic subunit of the third nuclear DNA polymerase, DNA polymerase II. POL2 expressed a transcript of approximately 7.5 kb and contained a reading frame that encoded a protein of calculated Mr 255,649. The N-terminal half of the predicted protein displayed relatively weak similarity of sequence to eukaryotic DNA polymerases. Disruption of the coding sequence at midpoint led to viable, slowly growing cells, which yielded a truncated polypeptide with DNA polymerase II activity, free from subunits B or C. Deletion of the reading frame resulted in inviability and the dumbbell terminal morphology that typically follows arrest of DNA replication. We conclude that three DNA polymerases are essential in yeast and argue that all three are replicases, a possibility that challenges existing models of eukaryotic DNA replication.
authors.affiliation: Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences Research Triangle Park, North Carolina 27709.
authors.forename: A
authors.initials: A
authors.lastname: Morrison
authors.forename: H
authors.initials: H
authors.lastname: Araki
authors.forename: A B
authors.initials: AB
authors.lastname: Clark
authors.forename: R K
authors.initials: RK
authors.lastname: Hamatake
authors.forename: A
authors.initials: A
authors.lastname: Sugino
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004257:DNA Polymerase II / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D015723:Gene Library; D004854:Herpesvirus 4, Human / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009154:Mutation; D015345:Oligonucleotide Probes; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D012689:Sequence Homology, Nucleic Acid; D013172:Spores, Fungal / Q000502:physiology
chemical_list: D015345:Oligonucleotide Probes; D004257:DNA Polymerase II