[The validation of an enzyme immunoassay for the detection of antibodies to bovine leukosis virus].
Validation of an enzyme immuno-assay for detection of antibodies against bovine leucosis virus is described in this paper. Internal standardisation of the test was done by means of a negative control serum. With absolute extinction of the negative control serum between 100 and 200 mE, a serum sample is rated positive, if its extinction is 1.5 times above the control. The methodological sensitivity of the enzyme immuno-assay described has proved to be four times as high as that of the immunodiffusion test. The results recorded at five diagnostic laboratories suggested a sensitivity of the test of 97.6 percent (92.1 to 100 percent) and a specificity of 98.1 percent (94.4 to 100 percent). The high efficiency of the test can be confirmed by immunoblotting.
Olechnowitz
A F
AF
Institut für Impfstoffe Dessau im Stammbetrieb des VEB Kombinat Veterinärimpfstoffe Dessau.
Miko
A
A
Koepernik
H
H
Starick
E
E
Fröbe
I
I
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D007124:Immunoenzyme Techniques; D007938:Leukemia / Q000175:diagnosis / Q000662:veterinary*; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D011237:Predictive Value of Tests; D012190:Retroviridae / Q000276:immunology*
D000914:Antibodies, Viral
Die Validierung eines Enzymimmunoassays zum Nachweis von Antikörpern gegen das Rinderleukosevirus.