Release of iron from diferric transferrin in the presence of rat liver plasma membranes: no evidence of a plasma membrane diferric transferrin reductase.
The transfer of iron from diferric transferrin to bathophenanthroline disulfonate was measured under varying conditions by spectrophotometry and EPR spectroscopy. Intact rat hepatocytes efficiently mediated the transfer of iron from human diferric transferrin to bathophenanthroline disulfonate. Isolated rat liver plasma membranes, in contrast, failed to facilitate the reaction at pH 7.4 in the presence of NADH, although the membranes were able to reduce ferricyanide and to oxidize NADH. Oxidation of NADH was stimulated by diferric transferrin. However, ferricyanide reductase and transferrin-stimulated NADH oxidase activities were apparently not linked to release of iron from transferrin. Our results, together with theoretical considerations, show that the ability (or inability) of intact cells or isolated plasma membranes to facilitate the transfer of iron from transferrin to strong diferric iron chelators does not allow interferences about the existence of an iron reduction step as part of the process of cellular uptake of iron from transferrin.
Thorstensen
K
K
Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461.
Aisen
P
P
D001059:Apoproteins / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002863:Chromogenic Compounds; D004578:Electron Spin Resonance Spectroscopy; D007501:Iron / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D010084:Oxidation-Reduction; D010618:Phenanthrolines; D011190:Potassium Cyanide / Q000494:pharmacology; D014168:Transferrin / Q000378:metabolism*
D001059:Apoproteins; D002863:Chromogenic Compounds; D010618:Phenanthrolines; D014168:Transferrin; C028284:apotransferrin; D009243:NAD; C017049:bathophenanthroline disulfonic acid; D007501:Iron; D009247:NADH, NADPH Oxidoreductases; C050129:diferric transferrin reductase; D011190:Potassium Cyanide