Estramustine phosphate reversibly inhibits an early stage during adenovirus replication.
Estramustine phosphate, an estradiol-mustard conjugate, was shown to reversibly inhibit a stage during the first hour of productive adenovirus 2 infection of HeLa cells. This drug, employed in the therapy of advanced prostatic cancer, specifically interacts with microtubule-associated proteins (MAPs) of the cytoskeleton. The results obtained under physiological conditions in vivo suggest a MAPs-interference with the microtubule-mediated vectorial migration of the virus inoculum to the nucleus. Virus attachment, uncoating kinetics and the appearance of established uncoating intermediates were not affected.
Everitt
E
E
Department of Microbiology, University of Lund, Sweden.
Ekstrand
H
H
Boberg
B
B
Hartley-Asp
B
B
D000257:Adenoviridae Infections / Q000382:microbiology; D000260:Adenoviruses, Human / Q000187:drug effects* / Q000502:physiology; D000956:Antigens, Viral / Q000096:biosynthesis; D002213:Capsid / Q000096:biosynthesis; D002499:Centrifugation, Density Gradient; D004961:Estramustine / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism*; D009588:Nitrogen Mustard Compounds / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
D000956:Antigens, Viral; D008869:Microtubule-Associated Proteins; D009588:Nitrogen Mustard Compounds; D004961:Estramustine