title: Protein factors that regulate the growth and differentiation of mouse myeloid leukaemia cells.
abstract: We have purified and characterized several protein factors that regulate the growth and differentiation of mouse myeloid leukaemia M1 cells. The differentiation factor (D-factor) from conditioned medium (CM) of Ehrlich ascites tumour cells is a glycoprotein of Mr 40,000-50,000. Its amino acid sequence was found to be almost identical to that of leukaemia inhibitory factor (LIF) from Krebs II ascites cells. The differentiation inhibitory factor (I-factor) from the CM of variant M1 cell clones which were resistant to several differentiation inducers is a basic protein of apparent Mr 68,000. The growth inhibitory factor (GI-factor) that specifically inhibits the partially differentiated and still growing monocytic leukaemia M1 cells was isolated from the CM of a clone of M1 cells resistant to the differentiation inducers. This GI-factor is a basic protein with an Mr of 25,000. Regulation by these protein factors together with other known cytokines of growth and differentiation of M1 cells is reported.
authors.affiliation: Department of Chemotherapy, Saitama Cancer Center Research Institute, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Hozumi
authors.forename: M
authors.initials: M
authors.lastname: Tomida
authors.forename: Y
authors.initials: Y
authors.lastname: Yamamoto-Yamaguchi
authors.forename: T
authors.initials: T
authors.lastname: Kasukabe
authors.forename: J
authors.initials: J
authors.lastname: Okabe-Kado
authors.forename: Y
authors.initials: Y
authors.lastname: Honma
authors.forename: M
authors.initials: M
authors.lastname: Hayashi
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D006131:Growth Inhibitors / Q000032:analysis / Q000502:physiology*; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D007951:Leukemia, Myeloid / Q000473:pathology*; D008222:Lymphokines / Q000032:analysis / Q000502:physiology*; D051379:Mice; D015291:Transforming Growth Factors / Q000502:physiology*
chemical_list: D006131:Growth Inhibitors; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D015291:Transforming Growth Factors