A dot-ELISA for the detection of IgG antibodies to mumps and varicella viruses.
An enzyme-linked immunosorbent assay using nitrocellulose strips (dot-ELISA) for the routine laboratory detection of IgG antibodies to mumps and varicella viruses is described. The virus antigens are dotted onto nitrocellulose strips, and the dotted strips are incubated with the sera to be tested. The bound antibodies are revealed using enzyme-labeled antihuman IgG antibodies. Reliable results are obtained when the assay is carried out at 37 degrees C. The reported data indicate that the dot-ELISA can reliably be used for the detection of IgG antibodies to mumps and varicella viruses in human sera.
Leonardi
M S
MS
Department of Microbiology, University of Messina, Italy.
Gazzara
D
D
Zummo
S
S
Fattal-German
M
M
Bizzini
B
B
Mastroeni
P
P
D000914:Antibodies, Viral / Q000032:analysis*; D003168:Complement Fixation Tests; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D014645:Herpesvirus 3, Human / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007074:Immunoglobulin G; D009109:Mumps virus / Q000276:immunology* / Q000302:isolation & purification
D000914:Antibodies, Viral; D007074:Immunoglobulin G