title: Evidence for LH-inhibiting activity in ovine peripheral and testicular blood.
abstract: Intracellular cyclic AMP and testosterone productions by purified mature rat Leydig cells were stimulated by oLH (25 micrograms/l) 18- and 12-fold, respectively, after a 5-h incubation period. The replacement of the incubation medium by charcoal-treated testicular venous plasma (40%, v/v) from adult rams in the breeding season induced an inhibition of cyclic AMP and testosterone productions (82 and 66%, respectively, of oLH-stimulated values). Testicular arterial plasma is less effective than testicular venous plasma, even when it originates from non-breeding season rams; in that case, testicular venous and arterial plasma strongly inhibit testosterone productions (84 and 67%, respectively of oLH-stimulated values), which probably indicates that the inhibitory activity is higher in the non-breeding season. The addition of peripheral plasma leads to a testosterone production equal to 35 and 65% of the oLH-stimulated values, respectively, for ram blood collected in non-breeding and breeding seasons. The same concentration of ovine testicular lymph or rete testis fluid is without significant effect on cyclic AMP production; however, testosterone is slightly decreased by lymph but enhanced by rete testis fluid. Increasing amounts of venous or arterial testicular blood induce a dose-related decrease of the specific binding of labelled hCG in both rat and ram testicular membranes. This inhibiting factor is present in peripheral and testicular blood of either control or hypophysectomized or castrated rams, is a protein in nature, heat-sensitive, and has an apparent molecular weight higher than 10,000 daltons.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Laboratoire de biochimie, URA CNRS 609, CHU Côte de Nacre, Caen, France.
authors.forename: V
authors.initials: V
authors.lastname: Papapdopoulos
authors.forename: P
authors.initials: P
authors.lastname: Kamtchouing
authors.forename: N
authors.initials: N
authors.lastname: Boujrad
authors.forename: C
authors.initials: C
authors.lastname: Pisselet
authors.forename: C
authors.initials: C
authors.lastname: Perreau
authors.forename: A
authors.initials: A
authors.lastname: Locatelli
authors.forename: M A
authors.initials: MA
authors.lastname: Drosdowsky
authors.forename: M T
authors.initials: MT
authors.lastname: Hochereau de Reviers
authors.forename: S
authors.initials: S
authors.lastname: Carreau
mesh_terms: D000818:Animals; D001947:Breeding; D006063:Chorionic Gonadotropin / Q000378:metabolism; D000242:Cyclic AMP / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D005298:Fertility; D066298:In Vitro Techniques; D007985:Leydig Cells / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008297:Male; D012621:Seasons; D012756:Sheep; D013737:Testis / Q000098:blood supply* / Q000378:metabolism; D013739:Testosterone / Q000096:biosynthesis
chemical_list: D006063:Chorionic Gonadotropin; D013739:Testosterone; D007986:Luteinizing Hormone; D000242:Cyclic AMP