Morphological and hormonal changes in the frog, Rana esculenta, testis after administration of ethane dimethane sulfonate.
Apart from mice, in rodents ethane dimethane sulfonate (EDS) selectively destroys Leydig cells. This has been indicated as a new method for the study of seminiferous interstitial compartment interaction. No information on the possible destruction and repopulation of Leydig cells exists in lower vertebrates. This study deals with EDS effects in the frog, Rana esculenta. Animals received a single intraperitonial dose (100 mg/kg body wt) and were sacrificed at 0, 12, and 24 hr and 3, 4, 7, 14, and 28 days postinjection. Androgens (testosterone + DHT) were measured in plasma and right testes. Moreover, left testes were fixed and examined for histological observation. Plasma androgen levels were extremely low on Day 4 after EDS treatment and remained unchanged thereafter. In testes, androgen levels decreased on Day 4 but increased to control levels on Day 14. Leydig cells were damaged within 3 days post-treatment and were completely destroyed on Days 4 and 5. Germinal compartment damage appeared only where the adjacent interstitial tissue presented complete destruction. Pale primary spermatogonia (stem cells) were always present. Testes restored to normal on Day 14 and spermatogenesis resumed to the regenerating interstitial tissue. These results show that regenerating testes in R. esculenta retain androgens and that interstitial-germinal compartment communications may have a role in maintaining spermatogenesis.
Minucci
S
S
Dipartimento di Fisiologia Umana e Funzioni Biologiche Integrate F. Bottazzi, I Facoltà di Medicina e Chirurgia, Università di Napoli, Italy.
Fasano
S
S
Di Matteo
L
L
Chieffi Baccari
G
G
Pierantoni
R
R
D000728:Androgens / Q000097:blood; D000818:Animals; D004958:Estradiol / Q000097:blood; D008297:Male; D008698:Mesylates / Q000494:pharmacology*; D011893:Rana esculenta; D013737:Testis / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D000728:Androgens; D008698:Mesylates; D004958:Estradiol; C002994:ethylene dimethanesulfonate