Cytoprotection against neutrophil derived hypochlorous acid: a potential mechanism for the therapeutic action of 5-aminosalicylic acid in ulcerative colitis.
The aim of the present study was to investigate the effects of 5-aminosalicyclic acid (5-ASA) on the cell injury mediated by activated neutrophils. We used a system constituted of neutrophils, triggered with phorbol myristate acetate, and 51Cr-labelled Daudi cells as targets. The results show that 5-ASA is capable of efficiently preventing neutrophil-mediated lysis. 5-ASA was up to 10-fold more effective than taurine, which acts as an hypochlorous acid scavenger. Moreover, 5-ASA was found to compete with taurine for the neutrophil derived hypochlorous acid. The results are consistent with the conclusion that 5-ASA is capable of limiting the neutrophil mediated cell damage by scavenging the generated hypochlorous acid. This may represent a potential mechanism for the therapeutic action of 5-ASA in ulcerative colitis.
Dallegri
F
F
First Medical Clinic, University of Genova Medical School, Italy.
Ottonello
L
L
Ballestrero
A
A
Bogliolo
F
F
Ferrando
F
F
Patrone
F
F
D000636:Aminosalicylic Acids / Q000494:pharmacology / Q000627:therapeutic use*; D002478:Cells, Cultured; D003093:Colitis, Ulcerative / Q000188:drug therapy*; D003601:Cytotoxicity Tests, Immunologic; D006801:Humans; D006997:Hypochlorous Acid / Q000037:antagonists & inhibitors*; D019804:Mesalamine; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D010101:Oxygen Consumption / Q000187:drug effects
D000636:Aminosalicylic Acids; D019804:Mesalamine; D006997:Hypochlorous Acid