title: Isolation and characterization of cellulolytic anaerobic fungi and associated mycoplasmas from the rumen of a steer fed a roughage diet.
abstract: The isolation of 12 strains of cellulolytic fungi from the rumen of a roughage-fed steer is described. These represented three different genera, including one new genus and species (Orpinomyces bovis). The organisms were indistinguishable on the basis of fermentation products from cellulose, and their fermentation patterns were very similar to those of rumen fungi isolated in other countries. Mycoplasmas were found to be associated with 7 of the 12 isolates. The ecological role of the association of the mycoplasmas with rumen fungi is still unknown.
authors.affiliation: Research Station, Agriculture Canada, Lethbridge, Alta.
authors.forename: H
authors.initials: H
authors.lastname: Kudo
authors.forename: K D
authors.initials: KD
authors.lastname: Jakober
authors.forename: R C
authors.initials: RC
authors.lastname: Phillippe
authors.forename: K J
authors.initials: KJ
authors.lastname: Cheng
authors.forename: D J
authors.initials: DJ
authors.lastname: Barr
authors.forename: J W
authors.initials: JW
authors.lastname: Costerton
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D002417:Cattle; D002482:Cellulose / Q000378:metabolism; D004043:Dietary Fiber; D005658:Fungi / Q000201:enzymology / Q000302:isolation & purification*; D008297:Male; D009174:Mycoplasma / Q000302:isolation & purification* / Q000648:ultrastructure; D012417:Rumen / Q000382:microbiology*
chemical_list: D004043:Dietary Fiber; D002482:Cellulose