title: Enhanced penetration of zona-free hamster ova by sperm prepared by Nycodenz and Percoll gradient centrifugation.
abstract: The effect of sperm penetration capacity after selection procedures using Percoll (Pharmacia AB, Uppsala, Sweden) and Nycodenz (Nycomed Diagnostics, Oslo, Norway) gradient centrifugation was compared with double-washed and swim-up in 47 subfertile men. The results of sperm motility, velocity, and amplitude lateral head displacement showed no significant improvement with the centrifugation procedures. The sperm penetration assay results obtained with double-washed and swim-up technique were poor (2.7% +/- 1.7%), however, a significant enhancement was obtained by Percoll (16.3% +/- 3.7%) and Nycodenz (15.8% +/- 3.3%) processing. Nycodenz centrifugation allowed sperm penetration of zona-free hamster ova at comparable rates to Percoll separation.
authors.affiliation: Huntington Reproductive Center, Huntington Memorial Hospital, Pasadena, California.
authors.forename: P
authors.initials: P
authors.lastname: Serafini
authors.forename: W
authors.initials: W
authors.lastname: Blank
authors.forename: C
authors.initials: C
authors.lastname: Tran
authors.forename: M
authors.initials: M
authors.lastname: Mansourian
authors.forename: T
authors.initials: T
authors.lastname: Tan
authors.forename: J
authors.initials: J
authors.lastname: Batzofin
mesh_terms: D000328:Adult; D000818:Animals; D002469:Cell Separation; D002499:Centrifugation, Density Gradient / Q000379:methods*; D006224:Cricetinae; D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000473:pathology / Q000503:physiopathology; D007472:Iohexol; D008297:Male; D008875:Middle Aged; D010063:Ovum / Q000502:physiology; D011205:Povidone; D012822:Silicon Dioxide; D013075:Sperm Capacitation / Q000502:physiology; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D013094:Spermatozoa / Q000166:cytology / Q000473:pathology / Q000502:physiology*
chemical_list: D007472:Iohexol; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone