Stability and localization of biotin-labeled murine monoclonal antibody to human gastric cancer in normal mice and nude mice-human tumor models.
We examined the tissue localization of biotin-labeled murine monoclonal antibody (MAb) S202 directed against the human scirrhous gastric carcinoma cell line MK-01 in normal and tumor-bearing mice after intravenous (IV) administration. The biotin-labeled MAb proved to be stable in vivo under normal conditions, antibody titer being 1:256 at 4 hr after IV injection. At 24 hr after injection, the tumor was stained by the avidin-biotin-peroxidase complex (ABC) method. Biotin-labeled MAb was found to be suitable for detection of the xenografted tumor of nude mice. This study provides new information concerning the dynamics of the distribution of biotin-labeled MAb in vivo.
Yokota
T
T
First Department of Surgery, Kyoto Prefectural University of Medicine, Japan.
Yamaguchi
T
T
Kitamura
K
K
Otsuji
E
E
Sawai
K
K
Takahashi
T
T
D002293:Adenocarcinoma, Scirrhous / Q000276:immunology* / Q000473:pathology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001710:Biotin; D002460:Cell Line; D004195:Disease Models, Animal; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000276:immunology / Q000473:pathology; D013274:Stomach Neoplasms / Q000276:immunology* / Q000473:pathology; D014183:Transplantation, Heterologous
D000911:Antibodies, Monoclonal; D001710:Biotin