Replication of HIV-1 in primary monocyte cultures.
A subpopulation of human monocytes began to express surface CD4 during its in vitro cultivation in the presence of recombinant M-CSF and became susceptible to infection with an isolate of HIV-1 [AD-87(M)], originally recovered from a seropositive individual by cocultivation with primary monocytes. Approximately 10% of the adherent cells synthesized CD4; a similar fraction was infectable with AD-87(M) and produced high levels of progeny particles. An interesting feature of the HIV-1 infection of primary monocytes was the detection of gp 160/120 inside but not on the surface of virus-producing cells. The implication of these results on HIV infection in vivo will be discussed.
Potts
B J
BJ
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Maury
W
W
Martin
M A
MA
D015152:Blotting, Northern; D015153:Blotting, Western; D015704:CD4 Antigens / Q000032:analysis / Q000235:genetics; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D015488:HIV Antigens / Q000032:analysis; D006679:HIV Seropositivity / Q000382:microbiology; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D009000:Monocytes / Q000382:microbiology*; D012333:RNA, Messenger / Q000032:analysis; D013601:T-Lymphocytes / Q000382:microbiology; D014764:Viral Proteins / Q000032:analysis; D014779:Virus Replication
D015704:CD4 Antigens; D015488:HIV Antigens; D012333:RNA, Messenger; D014764:Viral Proteins