Paraquat inhibits the lipid peroxidation caused by carbon tetrachloride in guinea pig liver microsome.
Pentane, which is an index of lipid peroxidation, was formed in a mixture of microsomes, carbon tetrachloride (CCl4) and NADPH. Trichloromethyl radical adduct of the N-tert-butyl-alpha-phenylnitrone-trichroromethyl (BPN) was also formed in the mixture with BPN. The formation of pentane and the radical adduct were reduced in the presence of paraquat. Furthermore a spectrophotometric study showed that substrate-induced difference spectra in the Soret region (Type I) caused by CCl4 and microsomes were changed after adding paraquat. We conclude that paraquat inhibits the lipid peroxidation mediated by the radical intermediate of CCl4 by inhibiting the mixed function oxidase system.
Sato
N
N
Department of Anesthesiology, Hiroshima University School of Medicine, Japan.
Fujii
K
K
Kawamoto
M
M
Yuge
O
O
Morio
M
M
D000818:Animals; D002251:Carbon Tetrachloride / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D006168:Guinea Pigs; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D009249:NADP / Q000494:pharmacology; D010269:Paraquat / Q000494:pharmacology*; D010420:Pentanes / Q000378:metabolism
D010420:Pentanes; C033353:pentane; D009249:NADP; D002251:Carbon Tetrachloride; D010269:Paraquat