Simian virus 40 large T-antigen-dependent DNA replication is activated by protein phosphatase 2A in vitro.
The simian virus 40 large T antigen (T) is a multifunctional phosphoprotein. We found that T-dependent simian virus 40 DNA replication is substantially inhibited by okadaic acid. This result suggests that DNA replication is activated by dephosphorylation in vitro. We show here that the target activated by dephosphorylation, which stimulates DNA replication, is T and that the phosphatase involved is protein phosphatase 2A.
Lawson
R
R
Imperial Cancer Research Fund, Clare Hall Laboratories, Herts, United Kingdom.
Cohen
P
P
Lane
D P
DP
D000469:Alkaline Phosphatase / Q000378:metabolism; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D002472:Cell Transformation, Viral; D004261:DNA Replication / Q000187:drug effects; D004988:Ethers, Cyclic / Q000494:pharmacology; D006367:HeLa Cells / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D019319:Okadaic Acid; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D054648:Protein Phosphatase 2; D013539:Simian virus 40 / Q000235:genetics* / Q000276:immunology
D000952:Antigens, Polyomavirus Transforming; D004988:Ethers, Cyclic; D019319:Okadaic Acid; D000469:Alkaline Phosphatase; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2