[Evaluation of the stability of vincristine binding to blood platelets by the method of spin labeling of platelet membrane].
N-(1-oxyl-2,2,6,6-tetramethylpriperyd-4-y) maleimide (MSL) labels SH or NH2 groups of blood platelets membrane proteins in covalent bonds. Then EPR spectrum of MSL labeled platelets solutions can be measured. Pre-incubation + of the platelets with vincristine prior to MSL labeling induces SH and NK2 groups blocking, decrease in MSL binding to the platelets membrane and EPR spectrum modification of labeled platelets solution. The vincristine effect on platelets membrane lasts until platelets disintegrate. These data confirm, the stability of vincristine bond with platelets membrane.
Górski
J
J
IV Kliniki Chorób Wewnetrznych Akademii Medycznej w Gdańsku.
Nowak
J
J
Bautembach
S
S
Czestochowska
E
E
Tymiński
W
W
D001792:Blood Platelets / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004355:Drug Stability; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000378:metabolism; D008954:Models, Biological; D011485:Protein Binding; D014750:Vincristine / Q000097:blood*
D008565:Membrane Proteins; D014750:Vincristine
Próba oceny trwałości wiazania winkrystyny z płytkami krwi metoda spinowego znakowania błony płytkowej.