Two sulphonated dye compounds which compete for inositol 1,4, 5-trisphosphate binding to rat liver microsomes: effects on 5'-phosphatase activity.
The ability of heparin to interact with the Ins 1,4,5-P3 receptor is dependent on its chain length and degree of sulphation. Here we report results obtained with two sulphonated dye compounds of known structures and molecular weights below 1000, cibacron blue and Patent blue. Both compounds compete for Ins 1,4,5-P3 binding to rat liver microsomes and also inhibit Ins 1,4,5-P3 5'-phosphatase activity in the same preparation. Comparison with the effects of heparin show these to be two separate actions of the compounds.
Bootman
M D
MD
Thrombosis Research Unit, Ciba-Geigy Pharmaceuticals, Horsham, W. Sussex, UK.
Pay
G F
GF
Rick
C E
CE
Tones
M A
MA
D000818:Animals; D015220:Calcium Channels; D004396:Coloring Agents; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D053496:Inositol 1,4,5-Trisphosphate Receptors; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear; D012394:Rosaniline Dyes / Q000494:pharmacology*; D014227:Triazines / Q000494:pharmacology*
D015220:Calcium Channels; D004396:Coloring Agents; D053496:Inositol 1,4,5-Trisphosphate Receptors; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D012394:Rosaniline Dyes; D014227:Triazines; C012842:Cibacron Blue F 3GA; D015544:Inositol 1,4,5-Trisphosphate; C035215:inositol triphosphate phosphomonoesterase; D010744:Phosphoric Monoester Hydrolases; C008769:sulfan blue