title: The complete nucleotide sequence of enterovirus type 70: relationships with other members of the picornaviridae.
abstract: Enterovirus type 70 (EV70) is the causative agent of acute haemorrhagic conjunctivitis and may also give rise to a rare neurological complication closely resembling poliomyelitis. The complete nucleotide sequence of the genome of EV70 has been determined from cDNA cloned in Escherichia coli. The genome consists of a 5' non-coding region of 726 nucleotides (nt), a long open reading frame of 6582 nt and a 3' non-coding region of 82 nt prior to the poly(A) tract. Comparison of the nucleotide sequence and the predicted amino acid sequence of the polyprotein with those published for other enteroviruses reveals sufficiently high similarity to predict antigenic regions and polyprotein cleavage sites. The P1 region of EV70 is as similar to those of the entero- as to those of the rhinoviruses, whereas the P2 and P3 regions are more closely related to the coxsackie B and swine vesicular disease viruses than other entero- or rhinoviruses.
authors.affiliation: A.F.R.C. Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, U.K.
authors.forename: M D
authors.initials: MD
authors.lastname: Ryan
authors.forename: O
authors.initials: O
authors.lastname: Jenkins
authors.forename: P J
authors.initials: PJ
authors.lastname: Hughes
authors.forename: A
authors.initials: A
authors.lastname: Brown
authors.forename: N J
authors.initials: NJ
authors.lastname: Knowles
authors.forename: D
authors.initials: D
authors.lastname: Booth
authors.forename: P D
authors.initials: PD
authors.lastname: Minor
authors.forename: J W
authors.initials: JW
authors.lastname: Almond
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004770:Enterovirus / Q000235:genetics*; D005814:Genes, Viral; D006860:Hydrogen Bonding; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins