title: Exercise modulates the insulin-induced translocation of glucose transporters in rat skeletal muscle.
abstract: Insulin and acute exercise (45 min of treadmill run) increased glucose uptake into perfused rat hindlimbs 5-fold and 3.2-fold, respectively. Following exercise, insulin treatment resulted in a further increase in glucose uptake. The subcellular distribution of the muscle glucose transporters GLUT-1 and GLUT-4 was determined in plasma membranes and intracellular membranes. Neither exercise nor exercise----insulin treatment altered the distribution of GLUT-1 transporters in these membrane fractions. In contrast, exercise, insulin and exercise----insulin treatment caused comparable increases in GLUT-4 transporters in the plasma membrane. The results suggest that exercise might limit insulin-induced GLUT-4 recruitment and that following exercise, insulin may alter the intrinsic activity of plasma membrane glucose transporters.
authors.affiliation: Division of Cell Biology, Hospital for Sick Children, Toronto, Ontario, Canada.
authors.forename: A G
authors.initials: AG
authors.lastname: Douen
authors.forename: T
authors.initials: T
authors.lastname: Ramlal
authors.forename: G D
authors.initials: GD
authors.lastname: Cartee
authors.forename: A
authors.initials: A
authors.lastname: Klip
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism; D003571:Cytochalasin B / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D005082:Physical Exertion / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007328:Insulin; D009004:Monosaccharide Transport Proteins; D003571:Cytochalasin B; D005947:Glucose