Isolation and characterization of the human liver ethanolamine kinase.
Ethanolamine kinase was purified from human liver to apparent homogeneity and its properties were studied. The minimum molecular weight of the purified enzyme was estimated by SDS-PAGE to 42,000 +/- 2000 Da. The molecular weight of the native enzyme determined by Superose 12 (FPLC) gel filtration was 87,000 +/- 4000 Da, suggesting that ethanolamine kinase in human liver consists of two chains of the same or almost the same molecular weight. The titration of the active site normality showed 1.03 +/- 0.05 active sites/chain. The Km value for ethanolamine was 0.25 +/- 0.01 mM.
Draus
E
E
Abteilung Allgemeine Pädiatrie und Universität Kiel, F.R.G.
Niefind
J
J
Vietor
K
K
Havsteen
B
B
D000255:Adenosine Triphosphate / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D002797:Choline Kinase / Q000378:metabolism; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D019856:Ethanolamine; D004983:Ethanolamines / Q000378:metabolism; D006801:Humans; D007526:Isoelectric Point; D007527:Isoenzymes / Q000032:analysis; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D008274:Magnesium / Q000378:metabolism; D008970:Molecular Weight; D010770:Phosphotransferases / Q000302:isolation & purification* / Q000378:metabolism; D017853:Phosphotransferases (Alcohol Group Acceptor)
D000596:Amino Acids; D004983:Ethanolamines; D007527:Isoenzymes; D046911:Macromolecular Substances; D019856:Ethanolamine; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); D002797:Choline Kinase; C020125:ethanolamine kinase; D008274:Magnesium