Inhibitory effects of kampo medicine on Epstein-Barr virus antigen induction by tumor promoter.
We have investigated the possible interaction of Japanese Kampo medicine; Shosaiko-to with Epstein-Barr virus (EBV) induction by TPA (12-O-tetra-decanoyl-phorbol-13-acetate) in EBV-genome harboring cells. The effect of ether extract of Shosaiko-to was also studied on the induction of EBV by superinfection with EBV from P3HR-1 cells (P3H-EBV). Concomitant treatment of EBV-latently-infected cells (Raji and A2L/AH) with TPA and ether extract resulted in effective inhibition of EBV-antigen induction. However, in the experiments to test the influence of ether extract on EBV induction by superinfecting Raji cells with P3H-EBV, ether extract did not affect the percentage of EBV-antigen-positive cells. These data indicate that Shosaiko-to is effective to inhibit antigen synthesis induced by chemical promoters without affecting EBV-antigen induction after superinfection.
Furukawa
M
M
Department of Otorhinolaryngology, School of Medicine, Kanazawa University, Japan.
Sakashita
H
H
Kamide
M
M
Umeda
R
R
D000956:Antigens, Viral / Q000096:biosynthesis; D002213:Capsid / Q000096:biosynthesis; D002273:Carcinogens / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004854:Herpesvirus 4, Human / Q000187:drug effects / Q000254:growth & development*; D010936:Plant Extracts; D014162:Transfection; D014407:Tumor Cells, Cultured; D014775:Virus Activation / Q000187:drug effects*
D000956:Antigens, Viral; D002273:Carcinogens; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C461320:shosaiko-to