Human papillomavirus type 16 DNA expresses a replication modulation factor in cos-1 cells.
Plasmid DNA including the simian virus 40 (SV40) origin of replication undergoes uncontrolled, runaway replication in cos-1 cells owing to the intracellular production of SV40 large T antigen. Covalent linkage of such plasmids with human papillomavirus type 16 (HPV-16) DNA was found to prevent runaway replication. Replication control was found to be dependent on the presence of HPV-16 DNA sequences including the E1 open reading frame and part of the non-coding region.
Hecht
E M
EM
Department of Microbiology, U.M.D.S. Guy's Campus, London Bridge, U.K.
Chesters
P M
PM
McCance
D J
DJ
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics* / Q000378:metabolism; D027383:Papillomaviridae / Q000235:genetics*; D010957:Plasmids; D013539:Simian virus 40 / Q000235:genetics; D014162:Transfection
D004279:DNA, Viral