title: Effect of sialoadenectomy on gastric mucosal integrity and growth in the rat. A time-course study.
abstract: The effect of removal of the submandibular-sublingual salivary gland (sialoadenectomy) has been examined in terms of the effects on the susceptibility of the gastric mucosa to the inflammatory and damaging actions of ethanol. In addition, the effects of sialoadenectomy on cell turnover in the gastric mucosa has been examined. Animals were examined at one to five weeks after removal of the submandibular-sublingual salivary gland complex. In response to 100% w/v ethanol, sialoadenectomized rat displayed greater hemorrhagic damage to the gastric mucosa than sham-operated control rats. The difference between sialoadenectomized and sham control rats was significant at two to five weeks after surgery. The rate of [3H]thymidine incorporation into gastric mucosa was significantly reduced in sialoadenectomized rats at one and two weeks. Mucosal DNA concentration was significantly reduced in sialoadenectomized rats at four and five weeks after surgery. Mucosal myeloperoxidase activity was greater in sialoadenectomized rats treated with ethanol compared to control animals, but this difference was only significant at two weeks after surgery. Sialoadenectomy was also associated with a reduction in duodenal and gastric mucosal levels of immunoreactive epidermal growth factor (EGF). Differences between sialoadenectomized and control rats were not significant until three to four weeks after surgery. These data indicate that sialoadenectomy was associated with an increase in the susceptibility of rat gastric mucosa to ethanol-mediated damage. Sialoadenectomy also resulted in a reduction in gastric mucosal growth and gastroduodenal mucosal levels of EGF. However, the influence of sialoadenectomy on the susceptibility of the rat gastric mucosa to ethanol-mediated damage occurred prior to any significant effect on mucosal DNA concentration or EGF levels.
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Western Ontario, London, Canada.
authors.forename: B L
authors.initials: BL
authors.lastname: Tepperman
authors.forename: B D
authors.initials: BD
authors.lastname: Soper
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis / Q000096:biosynthesis / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000032:analysis / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology; D005753:Gastric Mucosa / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D008297:Male; D009195:Peroxidase / Q000032:analysis / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013276:Stomach Ulcer / Q000139:chemically induced / Q000378:metabolism; D013361:Sublingual Gland / Q000502:physiology* / Q000601:surgery; D013363:Submandibular Gland / Q000502:physiology* / Q000601:surgery; D013997:Time Factors
chemical_list: D000431:Ethanol; D004815:Epidermal Growth Factor; D004247:DNA; D009195:Peroxidase