title: Flow cytometric detection of bovine viral diarrhea virus in peripheral blood leukocytes of persistently infected cattle.
abstract: Flow cytometry was investigated for detection of bovine viral diarrhea virus (BVDV) in peripheral blood mononuclear leukocytes of persistently infected cattle. The mononuclear leukocytes were purified by sedimentation in a gradient of Ficoll-Paque, fixed, permeabilized, and then labelled by indirect immunofluorescence using biotinylated immunoglobulins from a porcine antiserum to BVDV. Flow cytometric analysis of blood samples obtained from persistently infected cattle revealed virus in 3.0-21.0% (mean +/- SD, 11.2% +/- 6.4%) of the mononuclear leukocytes. Fluorescent cells were not observed in controls. Flow cytometric detection of BVDV in blood cells of persistently infected bovines is a rapid and objective technique which does not require cell culture facilities.
authors.affiliation: Animal Biotechnology Research Center, National Veterinary Laboratory, Copenhagen, Denmark.
authors.forename: P
authors.initials: P
authors.lastname: Qvist
authors.forename: B
authors.initials: B
authors.lastname: Aasted
authors.forename: B
authors.initials: B
authors.lastname: Bloch
authors.forename: A
authors.initials: A
authors.lastname: Meyling
authors.forename: L
authors.initials: L
authors.lastname: Rønsholt
authors.forename: H
authors.initials: H
authors.lastname: Houe
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000382:microbiology*; D002417:Cattle; D002469:Cell Separation; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology / Q000302:isolation & purification*; D005260:Female; D005434:Flow Cytometry; D007963:Leukocytes, Mononuclear / Q000382:microbiology*
chemical_list: D000956:Antigens, Viral