title: High frequency of Epstein-Barr virus genome detection in Hodgkin's disease of HIV-positive patients.
abstract: Lymph nodes obtained from 7 HIV-positive and 20 HIV-negative patients with Hodgkin's disease were examined for the presence of Epstein-Barr virus antigens and genome. EBV antigens were observed in only 2 out of 20 HIV-negative patients, whereas lymph nodes of HIV-positive patients did not reveal evidence of EBV antigens. By in situ hybridization and Southern blot analysis, EBV genome was found in 5 out of 7 HIV-positive patients; the EBV genome was detected in the nucleus of Reed-Sternberg and Hodgkin's cells. EBV DNA was observed by in situ hybridization and Southern blot analysis in only 3 out of 20 HIV-negative patients with Hodgkin's disease. In both groups, Reed-Sternberg and Hodgkin's cells were negative for C3d EBV receptor. Our results show a statistically significant increased expression of EBV DNA in HIV-positive patients with Hodgkin's disease, as compared with HIV-negative patients with HD.
authors.affiliation: Dip. Biopatologia Umana, Università La Sapienza, Rome, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Uccini
authors.forename: F
authors.initials: F
authors.lastname: Monardo
authors.forename: A
authors.initials: A
authors.lastname: Stoppacciaro
authors.forename: A
authors.initials: A
authors.lastname: Gradilone
authors.forename: A M
authors.initials: AM
authors.lastname: Aglianò
authors.forename: A
authors.initials: A
authors.lastname: Faggioni
authors.forename: V
authors.initials: V
authors.lastname: Manzari
authors.forename: L
authors.initials: L
authors.lastname: Vago
authors.forename: G
authors.initials: G
authors.lastname: Costanzi
authors.forename: L P
authors.initials: LP
authors.lastname: Ruco
mesh_terms: D000293:Adolescent; D000328:Adult; D015139:Blotting, Southern; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D015658:HIV Infections / Q000150:complications / Q000382:microbiology*; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006689:Hodgkin Disease / Q000209:etiology / Q000382:microbiology*; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization
chemical_list: D004279:DNA, Viral