Metals inhibit riboflavin-catalyzed generation of superoxide anion in vitro.
We found that addition of cationic metals inhibited flavin-catalyzed superoxide anion (O2-.) production in vitro. Inhibition of O2-. generation by metals appeared to relate to the ability of metal ions to chelate or complex with amine groups, altering their electronegativity. Metal inhibition of O2-. production has important implications for biological systems involving O2-. radical production, as well as for assays requiring the generation of O2-. in vitro.
Terada
L S
LS
Department of Medicine, University of Colorado Health Sciences Center, Denver.
Leff
J A
JA
Guidot
D N
DN
Shibao
G A
GA
Repine
J E
JE
D002384:Catalysis; D004492:Edetic Acid; D066298:In Vitro Techniques; D008670:Metals; D008715:Methionine; D009538:Nicotine; D012256:Riboflavin; D013481:Superoxides
D008670:Metals; D013481:Superoxides; D009538:Nicotine; D004492:Edetic Acid; D008715:Methionine; D012256:Riboflavin