Enhancement of human herpesvirus 6 replication in adult human lymphocytes by monoclonal antibody to CD3.
The effect of monoclonal antibody to CD3 on human herpesvirus 6 (HHV-6) replication was assessed in adult peripheral blood mononuclear cells (PBMC) and in cord blood mononuclear cells (CBMC) by anticomplement immunofluorescence and Southern blot hybridization. Infection of CBMC by HHV-6 was productive; however, little or no infection was observed with PBMC from adults exposed to the virus even if stimulated by phytohemagglutinin. Monoclonal antibody to CD3 strongly enhanced HHV-6 replicative cycles in PBMC from adults.
Kikuta
H
H
Department of Pediatrics, Hokkaido University School of Medicine, Sapporo, Japan.
Lu
H
H
Tomizawa
K
K
Matsumoto
S
S
D000911:Antibodies, Monoclonal / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D000956:Antigens, Viral / Q000096:biosynthesis; D015139:Blotting, Southern; D017252:CD3 Complex; D004279:DNA, Viral / Q000032:analysis; D005312:Fetal Blood / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology*; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D000956:Antigens, Viral; D017252:CD3 Complex; D004279:DNA, Viral; D011948:Receptors, Antigen, T-Cell