Novel instrumentation for multifield time-lapse cinemicrography.
The most significant feature of the system that is described is its ability to image essentially simultaneously the growth of up to 99 single cells into macroscopic colonies, each in its own microscope field. Operationally, fields are first defined and programmed by a trained observer. All subsequent steps are automatic and under computer control. Salient features of the hardware are stepper motor-controlled movement of the stage and fine adjustment of an inverted microscope, a high-quality 16-mm cine camera with light meter and controls, and a miniature incubator in which cells may be grown under defined conditions directly on the microscope stage. This system, termed MUTLAS, necessitates reordering of the primary images by rephotographing them on fresh film. Software developed for the analysis of cell and colony growth requires frame-by-frame examination of the secondary film and the use of a mouse-driven cursor to trace microscopically visible (4X objective magnification) events.
Department of Radiation Oncology, Stanford University Medical School, California 94305.
R F
RF
Kallman
N
N
Blevins
M A
MA
Coyne
S D
SD
Prionas
D000818:Animals; D002478:Cells, Cultured; D003584:Cytological Techniques / Q000295:instrumentation*; D004867:Equipment Design; D007942:Leukemia, Experimental / Q000473:pathology; D051379:Mice; D008853:Microscopy / Q000295:instrumentation*; D009040:Motion Pictures / Q000295:instrumentation*; D010781:Photography / Q000379:methods; D012815:Signal Processing, Computer-Assisted; D012984:Software