A major outer membrane protein of Serratia marcescens which was easily solubilized and had a capacity to bind to calcium.
Easily solubilized major outer membrane protein was found in Serratia marcescens. The protein was originally obtained as a membrane-associated, insoluble protein in the outer membrane when the cells were slightly disrupted. However, the amount of this protein in the outer membrane gradually decreased with the time of sonication. The decrease was not due to decomposition of the protein but to solubilization into a soluble fraction after a long period of disruption. The molecular weight of this protein was 47 kDa and it bound calcium. Another 40 kDa calcium binding protein was also found in the outer membrane of S. marcescens.
Tada
Y
Y
Department of Bacteriology, Kinki University School of Medicine, Osaka, Japan.
Yamaguchi
J
J
D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D012706:Serratia marcescens / Q000378:metabolism*; D012995:Solubility
D001425:Bacterial Outer Membrane Proteins; D002135:Calcium-Binding Proteins; D002118:Calcium