Cleavage of immunoglobulins by moojeni protease A, from the venom of Bothrops moojeni.
Moojeni protease A, a proteolytic enzyme isolated from the venom of Bothrops moojeni hydrolyzes human and rabbit IgGs. The resulted fragments retained the combining though not the precipitating power or the property to fix complement. Similar to papain, moojeni protease A releases directly Fab fragments from IgG. In contrast to papain, however, the enzyme does not require the presence of thiol compounds either for activation or for reduction of the disulphide inter-heavy chain bridges. On the contrary, moojeni protease A is a metalloenzyme inhibited in the presence of thiol compounds.
Serviço de Bioquímica, Instituto Butantan, São Paulo, Brasil.
M T
MT
Assakura
F R
FR
Mandelbaum
D000818:Animals; D003435:Crotalid Venoms / Q000032:analysis*; D006801:Humans; D006868:Hydrolysis; D007136:Immunoglobulins / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000032:analysis / Q000494:pharmacology*; D011817:Rabbits
D003435:Crotalid Venoms; D007136:Immunoglobulins; D010447:Peptide Hydrolases