Effects of isoxazolyl-naphthoquinoneimines on growth and oxygen radical production in Trypanosoma cruzi and Crithidia fasciculata.
Several 4-(aminomethylisoxazolyl)-1,2-naphthoquinones inhibited growth and DNA synthesis in Trypanosoma cruzi and stimulated O2 uptake and O2-. generation by the parasite epimastigotes and their mitochondrial and microsomal membranes; these results support the idea that oxygen radicals play a role in quinone toxicity. Maximal effects on respiration and O2-. generation were observed with antimycin-inhibited cells. Similar results as well as stimulation of H2O2 production were obtained with Crithidia fasciculata despite the presence of catalase in this organism.
de Tarlovsky
M N
MN
Bioenergetics Research Centre, School of Medicine, University of Buenos Aires, Argentina.
Goijman
S G
SG
Molina Portela
M P
MP
Stoppani
A O
AO
D000818:Animals; D000981:Antiprotozoal Agents; D003421:Crithidia / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000378:metabolism; D007097:Imines / Q000494:pharmacology*; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism; D007555:Isoxazoles / Q000494:pharmacology*; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009285:Naphthoquinones / Q000494:pharmacology*; D010080:Oxazoles / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism*; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
D000981:Antiprotozoal Agents; D005609:Free Radicals; D007097:Imines; D007555:Isoxazoles; D009285:Naphthoquinones; D010080:Oxazoles; D004247:DNA; D006861:Hydrogen Peroxide; D010100:Oxygen