Eukaryotic DNA ligases.
Recent studies on eukaryotic DNA ligases are briefly reviewed. The two distinguishable enzymes from mammalian cells, DNA ligase I and DNA ligase II, have been purified to homogeneity and characterized biochemically. Two distinct DNA ligases have also been identified in Drosophila melanogaster embryos. The genes encoding DNA ligases from Schizosaccharomyces pombe, Saccharomyces cerevisiae and vaccinia virus have been cloned and sequenced. These 3 proteins exhibit about 30% amino acid sequence identity; the 2 yeast enzymes share 53% amino acid sequence identity or conserved changes. Altered DNA ligase I activity has been found in cell lines from patients with Bloom's syndrome, although a causal link between the enzyme deficiency and the disease has not yet been proven.
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts., Great Britain.
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Lasko
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Tomkinson
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Lindahl
D000595:Amino Acid Sequence; D000818:Animals; D001816:Bloom Syndrome / Q000201:enzymology; D011088:DNA Ligases / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology; D004261:DNA Replication; D006801:Humans; D008322:Mammals; D008969:Molecular Sequence Data; D011118:Polynucleotide Ligases / Q000378:metabolism*; D012440:Saccharomyces / Q000201:enzymology; D012689:Sequence Homology, Nucleic Acid; D014616:Vaccinia virus / Q000235:genetics
D011088:DNA Ligases; D011118:Polynucleotide Ligases