Quantitation of immunoglobulins produced by donor lymphocytes in transplant patients and the possible identification of donor cell-produced antiviral antibodies by imprint immunofixation.
In the present study two patients were studied in detail to estimate the quantity of IgG of donor lymphocyte origin. Using IgG subclass quantitation and Gm haemagglutination inhibition titre, we estimated that up to 3% of IgG found after transplantation may be of donor lymphocyte origin. Analysis of viral antibody patterns by imprint immunofixation suggested polyclonal production of donor-derived antibodies.
Red Cross and National Hospital Blood Centre, National Hospital, Oslo, Norway.
O
O
Flesland
B G
BG
Solheim
O J
OJ
Mellbye
B
B
Vandvik
D A
DA
Albrechtsen
D000328:Adult; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000276:immunology; D005260:Female; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000276:immunology; D007129:Immunoglobulin Gm Allotypes / Q000096:biosynthesis / Q000276:immunology; D007158:Immunologic Techniques; D016030:Kidney Transplantation / Q000276:immunology*; D017710:Lymphocyte Transfusion; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007129:Immunoglobulin Gm Allotypes