The ATP-dependent post translational modification of ferredoxin: NADP+ oxidoreductase.
Incubation of thylakoids with purified FNR and [32P]ATP led to the incorporation of phosphate into the FNR. In the absence of added FNR, 32P-labelled FNR could be detected associated with the thylakoids. An amino-acid analysis showed that in the dark, the FNR could be phosphorylated on a serine residue. In the presence of thylakoids, the FNR contained a threonine phosphate which was associated with a light-dependent reaction. The physiological function of this phosphorylation is not clear. Some modifications in NADP(+)-dependent photosystem I (PSI) activity and FNR-membrane association have been observed on the addition of ATP. Whether these changes are linked to the phosphorylation of the FNR remain to be fully elucidated.
Hodges
M
M
Laboratoire de Physiologie Végétale Moléculaire, CNRS (UA1128), Université de Paris Sud, Orsay, France.
Miginiac-Maslow
M
M
Le Maréchal
P
P
Rémy
R
R
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D002734:Chlorophyll / Q000235:genetics / Q000378:metabolism*; D002736:Chloroplasts / Q000201:enzymology*; D007887:Fabaceae / Q000378:metabolism*; D005287:Ferredoxin-NADP Reductase / Q000235:genetics / Q000378:metabolism*; D008027:Light; D045342:Light-Harvesting Protein Complexes; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D010084:Oxidation-Reduction; D010766:Phosphorylation; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D010946:Plants, Medicinal; D011499:Protein Processing, Post-Translational
D045342:Light-Harvesting Protein Complexes; D008565:Membrane Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D010940:Plant Proteins; C042085:thylakoid membrane phosphoprotein, plant; D002734:Chlorophyll; D000255:Adenosine Triphosphate; D005287:Ferredoxin-NADP Reductase; D009247:NADH, NADPH Oxidoreductases