A yeast-Escherichia coli shuttle vector containing the M13 origin of replication.
A yeast-Escherichia coli shuttle vector containing the M13 origin of replication has been constructed. This vector allows selection and replication in both Saccharomyces cerevisiae and E. coli, as well as single-stranded packaging from E. coli upon infection with a helper phage. The presence of a polylinker with various unique restriction sites facilitates the cloning of desired genes.
Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290.
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D001483:Base Sequence; D003001:Cloning, Molecular; D003090:Coliphages / Q000235:genetics*; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D005822:Genetic Vectors; D008938:Mitosis; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics*