Virulent Escherichia coli strains for chicks bind fibronectin and type II collagen.
125I-fibronectin and 125I-collagen (type II) binding was detected in Escherichia coli strains isolated from chickens and poults. High fibronectin binding-strains also bind the 29 kD aminoterminal fragment of fibronectin. Binding properties in strain CK28 were partially characterized. The highest binding of 125I-fibronectin and 125I-collagen for strain CK28 was obtained with bacteria grown at 33 degrees C. Binding of 125I-fibronectin, its 125I-29 kD fragment, and 125I-collagen, was very rapid, reaching a maximum in 5 min. Binding of 125I-fibronectin and 125I-collagen was considerably inhibited by preincubation of bacteria with unlabelled fibronectin and unlabelled type I collagen respectively, but not inhibited with human immunoglobulin G or bovine serum albumin. Inhibition experiments showed that the reversibility of 125I-fibronectin binding was estimated at approximately 50%, while reversibility for 125I-collagen binding was higher than 90%. Receptors for fibronectin, its 29 kD fragment, and collagen were released from the bacterial surface by treatment at different temperatures, and surface material released at 100 degrees C inhibited binding. There was cross-inhibition for both fibronectin and collagen binding when unlabelled fibronectin and unlabelled collagen were used as inhibitors, suggesting that binding receptors for both proteins may be closely located.
Gonzalez
E A
EA
Departamento de Microbiología y Parasitología, Universidad de Santiago, Facultad de Veterinaria, Lugo, Spain.
Blanco
J
J
Baloda
S B
SB
Fröman
G
G
Dho
M
M
Lafont
J P
JP
Wadström
T
T
D000818:Animals; D001665:Binding Sites; D002645:Chickens; D003094:Collagen / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D005353:Fibronectins / Q000378:metabolism*; D006384:Hemagglutination; D011201:Poultry Diseases / Q000382:microbiology*; D013499:Surface Properties; D014774:Virulence
D005353:Fibronectins; D003094:Collagen