Transmission of exogenous genes into the chicken.
Injection of infectious non-replicating REV vector directly beneath the chicken blastoderm leads to infection of embryonic stem cells. Vector sequences are present in a variety of specialized tissues of embryos and mature birds derived from infected blastoderms. Breeding studies show that replication-defective REV vectors can transfer heritable, non-viral genetic information into the chicken germ line.
Amgen Inc., Thousand Oaks, CA 91320.
R A
RA
Bosselman
R Y
RY
Hsu
M J
MJ
Briskin
T
T
Boggs
S
S
Hu
M
M
Nicolson
L M
LM
Souza
J A
JA
Schultz
W
W
Rishell
R G
RG
Stewart
D000818:Animals; D030801:Animals, Genetically Modified; D002645:Chickens / Q000235:genetics*; D015757:Genes, rev; D005822:Genetic Vectors; D014162:Transfection