Effect of 6 months use of a dentifrice and oral rinse containing sanguinaria extract and zinc chloride upon the microflora of the dental plaque and oral soft tissues.
This study documented the effect upon the oral flora of twice daily brushing with a dentifrice containing 0.075% sanguinaria extract and 2% zinc chloride, followed by use of a mouthrinse containing 0.03% sanguinaria extract and 0.2% zinc chloride. Sixty subjects were randomly assigned to treatment or placebo groups and monitored in a 6-month double-blind clinical trial. Bacteriological samples from the tongue, buccal mucosa, and supra- and subgingival plaque were characterized at 0, 14, and 28 weeks. Microbiological monitoring showed no increases in populations of yeast, staphylococci, coliform organisms, or Pseudomonas. Total Gram-negative counts in supragingival plaque samples decreased 83% in the active group compared to a 232% increase for the control group. Populations of B. intermedius in supragingival plaque were significantly lower in the active group at 3 months and significantly lower counts of Fusobacterium sp. were observed at 3 and 6 months. Results indicate that use of the test products did not promote opportunistic overgrowth of pathogens in the oral flora. Additionally, the alterations in organisms associated with gingivitis may account for reductions in gingivitis seen in the active group.
Fairleigh Dickinson University, Oral Health Research Center, Hackensack, NJ.
D S
DS
Harper
L J
LJ
Mueller
J B
JB
Fine
J
J
Gordon
L L
LL
Laster
D000328:Adult; D000470:Alkaloids / Q000627:therapeutic use*; D000704:Analysis of Variance; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D001419:Bacteria / Q000187:drug effects* / Q000302:isolation & purification; D053119:Benzophenanthridines; D002712:Chlorides / Q000627:therapeutic use*; D002986:Clinical Trials as Topic; D003773:Dental Plaque / Q000382:microbiology*; D003802:Dentifrices / Q000627:therapeutic use*; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D007546:Isoquinolines; D008297:Male; D009061:Mouth Mucosa / Q000382:microbiology*; D009067:Mouthwashes / Q000627:therapeutic use*; D010919:Placebos; D011549:Pseudomonas / Q000187:drug effects / Q000302:isolation & purification; D011897:Random Allocation; D013291:Streptococcus / Q000187:drug effects / Q000302:isolation & purification; D014059:Tongue / Q000382:microbiology; D015032:Zinc / Q000627:therapeutic use*; D017967:Zinc Compounds
D000470:Alkaloids; D000900:Anti-Bacterial Agents; D053119:Benzophenanthridines; D002712:Chlorides; D003802:Dentifrices; D007546:Isoquinolines; D009067:Mouthwashes; D010919:Placebos; D017967:Zinc Compounds; C016837:zinc chloride; C005705:sanguinarine; D015032:Zinc