From gene to phenotype in Drosophila and other organisms.
The growing number of cloned eukaryotic genes lacking a defined or proven biological function poses a major challenge in 'reverse genetics'. A method is described here that permits efficient screening for new lesions in, or close to, genes corresponding to cloned DNA sequences of interest. The technique involves transposon mutagenesis, followed by screening of DNA isolated from a population of mutagenised individuals (or their progeny) for evidence that the population contains at least one individual in which transposon insertion has occurred at the target locus. Detection of rare individuals within the population is facilitated by the use of the polymerase chain reaction (PCR). Once recognised, specific individuals (or their progeny) are isolated from the population by a process of sib-selection. In cases where insertion of the transposon has occurred close to, but not within, the target locus, secondary events involving imprecise excision of the transposon will nonetheless allow the isolation of mutant individuals. Though the method was developed specifically for the transposon-mutagenesis of Drosophila, extensions to other organisms and to other mutagenic strategies are feasible and some of the possibilities are discussed.
Kaiser
K
K
Department of Genetics, University of Glasgow, Scotland.
D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D004330:Drosophila / Q000235:genetics*; D005796:Genes; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods