[Methods of measuring the transmembrane electrical potential in cells].
The methods of intracellular microelectrodes, penetrating ions and potential-sensitive fluorescent probes are considered for their possibility to be used for quantitative estimation of transmembrane electrical potentials (TMP) of small cells (mainly through the example of lymphocytes). The following fluorescent methods are described in detail: separate measurement of two TMP components--potentials on the plasma and mitochondrial membranes of a cell; recording of individual differences of cells according to the TMP value. It is supposed that heterogeneity of cells by the TMP value (in particular, the presence of depolarized cells) may be responsible for errors and divergences of the TMP mean values measured by different methods.
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Kosnikov
D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D008564:Membrane Potentials; D008839:Microelectrodes; D011247:Pregnancy
D005456:Fluorescent Dyes
Metody izmereniia transmembrannykh élektricheskikh potentsialov v kletke.