Establishment of a human undifferentiated thyroid cancer cell line producing several growth factors and cytokines.
A cell line was established from undifferentiated giant cell carcinoma of the thyroid. The authors obtained cells from a 44-year-old patient admitted because of a rapidly growing anterior neck mass. The patient had significant leukocytosis and hypercalcemia shortly before her death. An autopsy revealed epidermoid metaplasia of the tumor cells. The cells (HTC/C3) had lost most of their differentiated functions. However, their thyroid nature was shown by peroxidase staining and by enzyme-linked immunostaining with Hashimoto patients' sera. The tumor extract was found to contain parathyroid (PTH)-like activity. Significant amounts of colony stimulating factor (CSF), which was further defined to be GM-CSF, and interleukin-1 alpha (IL-1 alpha) were detected in the conditioned media. Epidermal growth factor (EGF) binding to the HTC/C3 showed rich EGF receptors. Furthermore, the conditioned medium inhibited the binding of 125I-mEGF to HeLa cells, and transforming growth factor (TGF) was found repeatedly in the media.
Enomoto
T
T
Second Division of Internal Medicine, Kyoto University School of Medicine, Japan.
Sugawa
H
H
Inoue
D
D
Miyamoto
M
M
Kosugi
S
S
Takahashi
T
T
Kitamura
N
N
Yamamoto
I
I
Konishi
J
J
Mori
T
T
D000328:Adult; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000378:metabolism; D003470:Culture Media / Q000032:analysis; D066246:ErbB Receptors / Q000378:metabolism; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D007375:Interleukin-1 / Q000378:metabolism; D010281:Parathyroid Hormone / Q000378:metabolism; D013964:Thyroid Neoplasms / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured
D003115:Colony-Stimulating Factors; D003470:Culture Media; D006133:Growth Substances; D007375:Interleukin-1; D010281:Parathyroid Hormone; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D066246:ErbB Receptors