Usefulness of two methods of isoelectric focusing for the erythrocyte acid phosphatase phenotypes determination in human bloodstains.
The authors tried to compare the usefulness of the isoelectric focusing of EAP in bloodstains on 0.2 mm polyacrylamide gel with their method of determination of the enzyme on 1 mm polyacrylamide gel. Both methods turned out to be useful but better results were obtained on 0.2 mm gel. Isoelectric focusing on the ultra-thin gel is more sensitive; it gives clear enzyme strips, takes less time (30 min) and demands about half the amount of material.
Department of Forensic Medicine, Medical Academy, Gdańsk, Poland.
R
R
Pawlowski
S
S
Raszeja
G
G
Komocka
S
S
Kuczyński
D000135:Acid Phosphatase / Q000097:blood*; D001801:Blood Stains; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000379:methods*; D007527:Isoenzymes / Q000097:blood; D010641:Phenotype; D012016:Reference Values
D007527:Isoenzymes; D000135:Acid Phosphatase