Direct estimation of serological H-Y antigen by flow cytometry.
This study was undertaken to evaluate the efficiency of flow cytometry in the detection of the serological H-Y antigen, and to survey expression of H-Y in the normal human population. Peripheral blood leukocytes (granulocytes) were reacted with monoclonal H-Y antibody, gw-16, and with FITC-conjugated goat anti-mouse IgG, and then were scored for fluorescence in the flow cytometer. Assigned H-Y phenotype was correlated accurately with sex phenotype in 33 out of 38 women and 62 out of 65 men. Data were evaluated by exploratory data analysis (EDA).
College of Agricultural and Life Sciences, University of Wisconsin, Madison 53706-1284.
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M
Kent
S
S
Wachtel
H T
HT
Thaler
D000911:Antibodies, Monoclonal; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D005455:Fluorescent Antibody Technique; D006098:Granulocytes / Q000032:analysis*; D006182:H-Y Antigen / Q000032:analysis*; D006801:Humans; D008297:Male; D010641:Phenotype; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012732:Sex Determination Analysis
D000911:Antibodies, Monoclonal; D006182:H-Y Antigen