A biochemical study of serine proteinase activities at local gingival tissue sites in human chronic periodontitis.
Serine proteinases have the potential to influence the degradation of connective tissue in chronic periodontitis, which may progress episodically at individual tooth sites. Elastase-, chymotrypsin- and tryptase-like proteinase activity in homogenized gingival tissue were measured using, respectively, the selective peptide substrates MeOSuc-Ala-Ala-Pro-Val-AFC. MeOSuc-Phe-Pro-Phe-AFC and Z-Ala-Arg-Arg-AFC. Each tooth site was assayed separately and divided, where appropriate, into gingival tissue and granulomata. Elastase-like activity was detected in only about half of the sites and with large variations. Chymotrypsin-like activity decreased with increasing pocket depth, clinical attachment level, gingival index and gingival bleeding index. Tryptase-like activity did not vary consistently with clinical measures. Chymotrypsin- and tryptase-like proteinase activity were much higher in gingival tissue than in granulomata. These effects are best explained by the likely influence (or lack of influence) of the endogenous serum and tissue inhibitors of serine proteinases, the different cellular origins of the enzymes, and their relative affinities for their substrates.
Periodontal Department, King's College School of Medicine & Dentistry, Denmark Hill, London, England.
B M
BM
Eley
S W
SW
Cox
D000328:Adult; D002908:Chronic Disease; D002918:Chymotrypsin / Q000378:metabolism; D005260:Female; D005881:Gingiva / Q000201:enzymology*; D005888:Gingival Pocket / Q000201:enzymology; D005891:Gingivitis / Q000201:enzymology; D006099:Granuloma / Q000201:enzymology; D006801:Humans; D008297:Male; D008875:Middle Aged; D010196:Pancreatic Elastase / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D010512:Periodontal Index; D010518:Periodontitis / Q000201:enzymology*; D012697:Serine Endopeptidases / Q000032:analysis / Q000378:metabolism*
D010447:Peptide Hydrolases; C023073:tosylarginine methyl ester hydrolase; D012697:Serine Endopeptidases; D002918:Chymotrypsin; D010196:Pancreatic Elastase