A rapid microassay for hemolytic antibody--its application to immunotoxicological study.
A rapid microassay for hemolytic antibody using an automatic spectrophotometer was developed. This microassay was parallel to hemolytic plaque forming cell (PFC) assay most commonly used to detect antibody formation. The former had more advantages than the latter did. The data obtained using arsenic and some metallic compounds as immunomodifiers suggested that this microassay was suitable for the screening of immunomodifiers in immunotoxicological and immunopharmacological studies which deal with a large number of samples within limited time.
Yoshida
T
T
Department of Environmental Medicine and Occupational Health, Tokai University School of Medicine, Kanagawa, Japan.
Shimamura
T
T
Shigeta
S
S
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D001151:Arsenic / Q000633:toxicity; D006462:Hemolytic Plaque Technique; D007166:Immunosuppressive Agents / Q000032:analysis*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013053:Spectrophotometry
D007166:Immunosuppressive Agents; D001151:Arsenic