Mutagenic activity of pyrolysates of cyanocobalamin and some other water-soluble vitamins in the model system with the Salmonella/mammalian microsomes.
Pyrolysates of cyanocobalamin, thiamine hydrochloride, riboflavin, pyridoxine hydrochloride, and ascorbic acid were tested for mutagenicity in the histidine-requiring mutants Salmonella typhimurium TA98 and TA100. Each vitamin was sealed in a glass tube and heated at 100-600 degrees C in a muffle furnace. Methanol-chloroform extracts of the pyrolysate of each vitamin tested did not show any mutagenicity in either TA98 or TA100 without rat liver 9000 x g supernatant fraction (S9) added. In the presence of S9, the B-group vitamins (cyanocobalamin, thiamine hydrochloride, riboflavin, and pyridoxine hydrochloride) were all mutagenic in TA98 and TA100, with the highest activity among the vitamins tested found in the pyrolysate of cyanocobalamin. The pyrolysate of 0.25 mumole cyanocobalamin produced 3200 revertants, while the pyrolysates of 0.25 mumole thiamine hydrochloride and riboflavin produced only 910 revertants, and the pyrolysate of pyridoxine hydrochloride did not show any mutagenicity at that amount. The mutagenicity was generally more active to TA98 than to TA100, indicating that frameshift-type mutagens were contained in the pyrolysates. The pyrolysate of ascorbic acid did not show any mutagenic activity in either TA98 or TA100 under the present experimental conditions.
School of Pharmacy, Hokuriku University, Kanazawa, Japan.
R
R
Demura
S
S
Tsukada
N
N
Kotani
Y
Y
Tateoka
S
S
Narimatsu
I
I
Yamamoto
D000818:Animals; D001205:Ascorbic Acid / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D006358:Hot Temperature / Q000009:adverse effects*; D009152:Mutagenicity Tests; D051381:Rats; D012256:Riboflavin / Q000633:toxicity; D012486:Salmonella typhimurium; D013831:Thiamine / Q000633:toxicity; D014805:Vitamin B 12 / Q000633:toxicity*; D014803:Vitamin B Complex / Q000633:toxicity*
D014803:Vitamin B Complex; D014805:Vitamin B 12; D001205:Ascorbic Acid; D012256:Riboflavin; D013831:Thiamine