Identification, sequencing and expression of an integral membrane protein of the trans-Golgi network (TGN38).
Organelle-specific integral membrane proteins were identified by a novel strategy which gives rise to monospecific antibodies to these proteins as well as to the cDNA clones encoding them. A cDNA expression library was screened with a polyclonal antiserum raised against Triton X-114-extracted organelle proteins and clones were then grouped using antibodies affinity-purified on individual fusion proteins. The identification, molecular cloning and sequencing are described of a type 1 membrane protein (TGN38) which is located specifically in the trans-Golgi network.
European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
J P
JP
Luzio
B
B
Brake
G
G
Banting
K E
KE
Howell
P
P
Braghetta
K K
KK
Stanley
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000235:genetics; D005455:Fluorescent Antibody Technique; D006056:Golgi Apparatus / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D051381:Rats
D008562:Membrane Glycoproteins; D004247:DNA