Malignant haemangiopericytoma of lung: a cell culture and ultrastructural study.
Malignant haemangiopericytoma was grown in vitro to assess the histogenesis of the tumour. Disaggregated tumour cells maintained in serum-supplemented Waymouth's MB 752/1 medium showed monolayers of homogeneous spindle cells. Post-confluent cultures exhibited hillocks or plaques of multilayer growth within which considerable amount of basal lamina-like material and fibrillar matrix were present between cells. Delicate basal laminae were also expressed on cell surfaces facing matrix material. Other consistent features were attenuated cytoplasmic processes, desmosome-like junctions, abnormal mitochondria and a paucity of intracellular filaments. The three-dimensional organization of tumour cells with concomitant expression of differentiated phenotype in vitro has significant implications on the cell of origin and differentiation process of haemangiopericytoma.
Yang
A H
AH
Department of Pathology, Veterans General Hospital-Taipei, Taiwan, Republic of China.
Liu
H C
HC
D006393:Hemangiopericytoma / Q000648:ultrastructure*; D006801:Humans; D008175:Lung Neoplasms / Q000648:ultrastructure*; D008854:Microscopy, Electron; D014407:Tumor Cells, Cultured / Q000648:ultrastructure