Use of an alpha-D-glucosidase for the specific cytochemical identification of lateral alpha-D-xylosyl end groups in plant xyloglucans.
alpha-Linked D-xylosyl side chains represent the typical feature common to all xyloglucans not shared by other cell wall polysaccharides. Since no easily available alpha-D-xyloxidase is known, advantage was taken of the conformational and configurational homologies between alpha-D-xylopyranose and alpha-D-glucopyranose to make an alpha-D-glucosidase-gold complex which was able to recognize alpha-D-xylosyl terminal residues of xyloglucans. This marker was used together with alpha-L-fucosidase gold complex for the double labeling on two different structural features of the same macromolecule in plant primary cell wall.
Centre de Recherches sur les Macromolécules Végétales, C.N.R.S., Grenoble, France.
K
K
Ruel
J P
JP
Joseleau
D002473:Cell Wall / Q000032:analysis; D005936:Glucans; D006046:Gold; D006651:Histocytochemistry; D007202:Indicators and Reagents; D008854:Microscopy, Electron; D010944:Plants / Q000032:analysis* / Q000648:ultrastructure; D011134:Polysaccharides / Q000032:analysis*; D014990:Xylans; D014994:Xylose / Q000032:analysis*; D000520:alpha-Glucosidases; D005644:alpha-L-Fucosidase
D005936:Glucans; D007202:Indicators and Reagents; D011134:Polysaccharides; D014990:Xylans; C029353:xyloglucan; D006046:Gold; D014994:Xylose; D000520:alpha-Glucosidases; D005644:alpha-L-Fucosidase