Statistical design of ELISA protocols.
This paper shows how to obtain accuracy and efficiency in an ELISA analysis by allocating the wells on a 96-well microplate between calibration and determination of unknowns, and by choosing the known concentrations for calibration. The method also can determine how much is lost in precision by using a convenient but non-optimal protocol.
Graduate School of Management, University of California, Davis 95616.
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Bunch
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Rocke
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Harrison
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D013223:Statistics as Topic