Regulation of proliferation of embryonic heart mesenchyme: role of transforming growth factor-beta 1 and the interstitial matrix.
Proliferation of atrioventricular cushion mesenchyme of the embryonic avian heart maintained in three-dimensional aggregate culture is stimulated by interaction with the interstitial matrix. Chicken serum or transforming growth factor-beta 1, which stimulates proliferation, induces matrix deposition in regions of the aggregate showing high labeling indices with tritiated thymidine. Dispersed heart mesenchyme interstitial matrix introduced into serum-free culture is incorporated into the aggregate and stimulates cellular proliferation similar to serum or transforming growth factor-beta 1. Proliferation is reversibly inhibited by the peptide Gly-Arg-Gly-Asp-Ser-Pro. It is suggested that transforming growth factor-beta 1 stimulates the production of interstitial matrix and that a sufficient stimulus for proliferation in this system is the presence of the matrix, which acts as the adhesive support for cellular anchorage.
Choy
M
M
Department of Pediatrics, University of California Davis Medical Center, Sacramento 95817.
Armstrong
M T
MT
Armstrong
P B
PB
D000818:Animals; D001345:Autoradiography; D002449:Cell Aggregation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D003238:Connective Tissue / Q000187:drug effects / Q000196:embryology*; D003239:Connective Tissue Cells; D004261:DNA Replication / Q000187:drug effects; D005109:Extracellular Matrix / Q000502:physiology*; D006321:Heart / Q000196:embryology*; D013936:Thymidine / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014316:Tritium
D016212:Transforming Growth Factor beta; D014316:Tritium; D013936:Thymidine