Occurrence of 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 2-amino-6-methyldipyrido[1,2-a:3',2'-d]imidazole (Glu-P-1) and other heterocyclic amine mutagens in oil of charred egg yolk (ranyu).
Mutagenicity of oil of charred egg yolk (called ranyu in Japanese), which is commercially available and consumed as a health food in Japan, was tested on Salmonella typhimurium strains TA98 and TA100 with and without metabolic activation. Both strains showed a high response to the oil, and the number of His+ revertant colonies with strain TA98 was 15,000-20,000 for 1 g equivalent amount of oil. The mutagens were purified by acid extraction, chloroform extraction after alkalization, dialysis, adsorption to blue cotton, passing through a Sephadex LH-20 column and several stages of high-pressure liquid chromatography with reverse-phase columns. At least 7 heterocyclic amine mutagens were detected. Two of them were suggested to be 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) and 2-amino-6-methyldipyrido [1,2-a:3',2'-d]imidazole (Glu-P-1). One was suggested to be 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ). The others were not identified but distinguishable from 12 known heterocyclic amine mutagens. The estimated minimum contents of IQ and Glu-P-1 were 1.1 ng/g and 4.8 ng/g, respectively.
Tokyo College of Pharmacy, Japan.
T
T
Kato
K
K
Kikugawa
M
M
Asanoma
Y
Y
Sakabe
D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004530:Egg Yolk / Q000032:analysis*; D007093:Imidazoles / Q000032:analysis* / Q000302:isolation & purification / Q000633:toxicity; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis* / Q000302:isolation & purification; D009821:Oils / Q000008:administration & dosage / Q000032:analysis* / Q000494:pharmacology; D011804:Quinolines / Q000032:analysis* / Q000302:isolation & purification / Q000633:toxicity; D012486:Salmonella typhimurium / Q000235:genetics
D007093:Imidazoles; D009153:Mutagens; D009821:Oils; D011804:Quinolines; C029216:2-amino-3-methylimidazo(4,5-f)quinoline; C023785:2-amino-6-methyldipyrido(1,2-a-3',2'-d)imidazole