[Adjustable action of pi-shen recipe on immune function in mice with L1210 ascites].
Proliferation of Con A stimulated splenic lymphocyte was examined by incorporation of 3H-thymidine. Fluorescence polarization of DPH labelled splenic lymphocyte and bone marrow cells was measured. Proliferation of Con A stimulated splenic lymphocyte in DBA inbred healthy mice was higher than that of L1210 mice but fluorescence polarization of DPH labelled splenic lymphocyte in same healthy mice was lower than that of L1210 mice. i.e. membranous lipid lymphocyte fluidity of lymphocyte in the healthy mice was smaller than that of L1210 mice. 9 days after administration of Pi-Shen recipe by tube stomach proliferation of Con A-lymphocyte in the healthy mice has been increased. The recipe adjusted splenic lymphocyte membrane lipid fluidity of L1210 mice to level of those in healthy mice. Effects of Pi-Shen recipe on lipid fluidity of bone marrow cell membrane of L1210 mice were almost similar to that in splenic lymphocytes. These studies suggest that the mechanism on adjustable role of Pi-Shen recipe on T-lymphocyte function related to lymphocyte membrane lipid fluidity.
Dept. of Basic Medicine, Guang-An-Men Hospital, China Academy of TCM, Beijing.
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D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D016231:Fluorescence Polarization Immunoassay; D007939:Leukemia L1210 / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D008213:Lymphocyte Activation; D008560:Membrane Fluidity / Q000187:drug effects; D051379:Mice; D008811:Mice, Inbred DBA; D013601:T-Lymphocytes / Q000276:immunology*
D004365:Drugs, Chinese Herbal