Evidence that there are only two tRNA(Phe) genes in Escherichia coli.
pheV, one of the genes that code for tRNA(Phe), was deleted from the chromosome of a strain of Escherichia coli K-12. As a consequence of this mutation, expression of pheA, the gene for chorismate mutase P-prephenate dehydratase, the first enzyme in the terminal pathway of phenylalanine biosynthesis, was derepressed. Similar derepression of pheA has been reported in pheR mutants of E. coli K-12 (J. Gowrishankar and J. Pittard, J. Bacteriol. 150:1130-1137, 1982). Attempts to introduce a pheR mutation into the delta pheV strain failed under circumstances suggesting that this combination of mutations is lethal. Southern blot analysis of pheV+ and delta pheV strains indicated that there are only two tRNA(Phe) genes in E. coli. It is recommended that the names pheU and pheV be retained for these genes.
Pittard
J
J
Department of Microbiology, University of Melbourne, Parkville, Victoria Australia.
Praszkier
J
J
Certoma
A
A
Eggertsson
G
G
Gowrishankar
J
J
Narasaiah
G
G
Whipp
M J
MJ
D015139:Blotting, Southern; D002872:Chromosome Deletion; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005838:Genotype; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D012360:RNA, Transfer, Phe / Q000235:genetics*; D015183:Restriction Mapping
D004269:DNA, Bacterial; D012360:RNA, Transfer, Phe