Noradrenaline sensitivity and calcium fluxes in arteries from rabbits with perinephritis hypertension.
1. Contractions of isolated vascular and cardiac preparations taken from rabbits with perinephritis (one kidney, one wrapped) hypertension were compared with those of preparations from control operated animals. 2. Significantly increased sensitivity to noradrenaline, which acts on alpha 1-adrenoceptors, was found in mesenteric arterial rings but not in aortic rings. The degree of hypersensitivity was the same in the presence and absence of cocaine, suggesting that there is no increase in uptake of noradrenaline into adrenergic nerves in this model of hypertension. In contrast to these agonist-induced contractions, no increased sensitivity was found to potassium chloride, suggesting that hypersensitivity is specific for receptor mediated rather than membrane potential mediated effects. 3. No hypersensitivity to noradrenaline was found in the isolated left or right atria, which suggests that the hypertension is associated with changes in excitation-contraction coupling in blood vessels but not in cardiac muscle. 4. Hypertension increased basal 45Ca uptake in the mesenteric artery but not in the aorta. However, there was no significant difference between preparations from normotensive and hypertensive rabbits in 45Ca uptake or efflux stimulated by noradrenaline or KCl. 5. Increased basal 45Ca uptake could contribute to the increased sensitivity to noradrenaline found in the mesenteric artery in rabbit perinephritis hypertension.
Department of Physiology and Pharmacology, University of Strathclyde, Glasgow, United Kingdom.
M T
MT
Hall
R M
RM
Wadsworth
K A
KA
Kane
C A
CA
Hamilton
J L
JL
Reid
I W
IW
Rodger
D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D001011:Aorta; D002118:Calcium / Q000378:metabolism*; D003042:Cocaine / Q000494:pharmacology; D004146:Dioxanes / Q000494:pharmacology; D006978:Hypertension, Renovascular / Q000209:etiology / Q000503:physiopathology*; D019329:Idazoxan; D007668:Kidney / Q000503:physiopathology; D008297:Male; D008638:Mesenteric Arteries; D009119:Muscle Contraction / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000503:physiopathology; D009200:Myocardial Contraction / Q000187:drug effects*; D009638:Norepinephrine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D011189:Potassium Chloride / Q000494:pharmacology; D011224:Prazosin / Q000494:pharmacology; D011817:Rabbits; D013997:Time Factors
D004146:Dioxanes; D000804:Angiotensin II; D011189:Potassium Chloride; D003042:Cocaine; D002118:Calcium; D009638:Norepinephrine; D011224:Prazosin; D019329:Idazoxan