Prostatein C3-mRNA: a sensitive marker of androgen-responsiveness in prostate explant cultures.
Prostatein is an androgen-dependent protein which is secreted by the rat ventral prostate. To determine if prostatein or its mRNA were responsive to androgen in vitro, prostate explants were cultured in media containing 0 or 25 nM dihydrotestosterone (DHT), estradiol (E2), or cortisol (F). Prostatein concentrations in medium were measured by radioimmunoassay at 2 and 4 days and in homogenates at 4 days. They were not changed significantly by any of these steroids. The concentration of the mRNA for the C3-subunit of prostatein was determined by dot hybridization at 0, 2, 4, 6, and 8 days. It was decreased significantly by 2 days when compared with explants cultured in the presence of DHT and significant differences persisted through 8 days. In conclusion, quantitation of the mRNA for the C3-subunit of prostatein in short-term cultures of ventral prostate explants appears to be more sensitive to changes in androgen concentration than does measurement of prostatein, per se. Prostatein C3-mRNA may be a useful marker for in vitro studies of androgen agonists and antagonists.
Shao
T C
TC
VA Medical Center, Houston, TX 77030.
Kong
A
A
Young
C
C
Chu
W H
WH
Tindall
D J
DJ
Cunningham
G R
GR
D000727:Androgen-Binding Protein / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D015152:Blotting, Northern; D046508:Culture Techniques; D013196:Dihydrotestosterone / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology*; D005819:Genetic Markers / Q000032:analysis; D006854:Hydrocortisone / Q000494:pharmacology*; D007124:Immunoenzyme Techniques; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D011467:Prostate / Q000187:drug effects / Q000378:metabolism*; D060789:Prostatein; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D060765:Secretoglobins; D014598:Uteroglobin; D060788:Mammaglobin B
D000727:Androgen-Binding Protein; D013196:Dihydrotestosterone; D004958:Estradiol; D005819:Genetic Markers; D006854:Hydrocortisone; D060789:Prostatein; D012333:RNA, Messenger; D060765:Secretoglobins; D014598:Uteroglobin; C511484:Scgb1d2 protein, rat; C511483:Scgb1d4 protein, rat; C556468:Scgb2a1 protein, rat; D060788:Mammaglobin B