Detection of the trans activity of the plum pox virus NIa-like protease in infected plants.
The NIa-like protein of plum pox virus is a protease with high sequence specificity that is autocatalytically released from the viral polyprotein. In order to determine whether the protease is active in trans we constructed a fusion protein consisting of the C-terminal region of the plum pox virus polyprotein and the staphylococcal Protein A. The authentic protease recognition sequence Asn-Val-Val-Val-His-Gln-Ala occurs in the centre of this protein fusion. This protein was cleaved specifically by extracts of plum pox virus-infected plants due to the strong activity of the viral protease making it a useful tool for diagnostic purposes.
Institute of Applied Microbiology, University of Agriculture, Wien, Austria.
G
G
Himmler
S
S
Frank
H
H
Steinkellner
F
F
Rüker
D
D
Mattanovich
H W
HW
Katinger
D000595:Amino Acid Sequence; D010450:Endopeptidases; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000235:genetics*; D010942:Plant Viruses / Q000201:enzymology / Q000235:genetics*; D010944:Plants / Q000382:microbiology; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013205:Staphylococcal Protein A / Q000378:metabolism; D013379:Substrate Specificity; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
D011993:Recombinant Fusion Proteins; D013205:Staphylococcal Protein A; D014764:Viral Proteins; D010450:Endopeptidases; D010447:Peptide Hydrolases; C059992:nuclear inclusion protein a, mosaic viruses