c-Ki-ras mutations in chronic ulcerative colitis and sporadic colon carcinoma.
Mutations in the first exon of the c-Ki-ras protooncogene were analyzed in carcinomas and dysplasias from patients with sporadic colon cancer and chronic ulcerative colitis by a combination of histological enrichment, cell sorting, polymerase catalyzed chain reaction, and direct sequencing. In contrast to sporadic colon carcinomas, where 52% (11 of 21) contained mutations in codon 12, only 1 of 28 samples of ulcerative colitis associated carcinoma or dysplasia contained a c-Ki-ras mutation, despite the presence of aneuploid cell populations. These results suggest that a different genetic pathway for tumor progression may exist between sporadic colon carcinoma and carcinomas arising in chronic ulcerative colitis.
Department of Pathology, University of Washington School of Medicine, Seattle, Washington.
G C
GC
Burmer
D S
DS
Levine
B G
BG
Kulander
R C
RC
Haggitt
C E
CE
Rubin
P S
PS
Rabinovitch
D000328:Adult; D000368:Aged; D000782:Aneuploidy; D002469:Cell Separation; D003093:Colitis, Ulcerative / Q000235:genetics*; D003110:Colonic Neoplasms / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry; D011905:Genes, ras; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D011519:Proto-Oncogenes