Comparison of doubly labeled water, intake-balance, and direct- and indirect-calorimetry methods for measuring energy expenditure in adult men.
Energy expenditure (EE) of four adult men on a weight-maintenance diet was estimated by use of doubly labeled water, intake balance, and direct and indirect calorimetry. The doubly labeled water (2H218O) method was used to estimate free-living EE for 13 d. Metabolizable energy (ME) intake was used to estimate free-living EE for 1 wk. The subjects' 24-h EE was measured in a dual direct-indirect room calorimeter on 3 alternate days. Estimates of free-living EE as measured by ME intake and doubly labeled water indicate agreement between the two methods (mean difference +/- SEM, -1.04 +/- 0.63%). Measurements of EE with indirect and direct calorimetry are equivalent (mean difference 0.63 +/- 0.44%). The daily EE measured by doubly labeled water in these free-living adults over a 13-d period was 15.01% greater than the 24-h EE measured within the calorimeter.
US Department of Agriculture, Energy and Protein Nutrition Laboratory, Beltsville, MD 20705.
J L
JL
Seale
W V
WV
Rumpler
J M
JM
Conway
C W
CW
Miles
D000203:Activities of Daily Living; D000328:Adult; D001823:Body Composition; D001834:Body Water / Q000378:metabolism*; D001835:Body Weight; D002151:Calorimetry; D002153:Calorimetry, Indirect; D002149:Energy Intake / Q000502:physiology*; D004734:Energy Metabolism / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011865:Radioisotope Dilution Technique