Protein synthesis in bone and skin of the rat are inhibited by ethanol: implications for whole body metabolism.
The acute effects of ethanol (75 mmol/kg body weight, intraperitoneal) on rates of protein synthesis in bone tibia) and skin of young (approximately 100 g body weight) laboratory rats was investigated. Plasma ethanol levels were raised to approximately 40 mmol/liter. At 2.5 hr, rates of protein synthesis were measured with a flooding dose of L-[4-3H]phenylalanine. In bone the protein-bound specific radioactivities, fractional synthesis rates, and synthesis rates relative to RNA and DNA were significantly reduced by approximately 30%. In skin these variables similarly decreased in response to ethanol treatment, by approximately 25%. The reduction in absolute rates of protein synthesis in bone (delta, 0.7 g protein/day/kg body weight) and skin (delta, 3.4 g protein/day/kg body weight) were comparable to the reductions in liver and skeletal muscle in response to acute ethanol. As bone and skin contribute to a quarter of whole body protein synthesis, it was concluded that these observations may have important implications for whole body protein homeostasis.
Preedy
V R
VR
Department of Clinical Biochemistry, King's College School of Medicine and Dentistry, London, United Kingdom.
Marway
J S
JS
Salisbury
J R
JR
Peters
T J
TJ
D000818:Animals; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000493:pharmacokinetics / Q000494:pharmacology*; D008297:Male; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000187:drug effects* / Q000378:metabolism
D000431:Ethanol