Evaluation of five different methods for routine diagnosis of rabies.
Brain tissue from 187 animals of different species was investigated by means of fluorescent antibody test, peroxidase anti-peroxidase technique, mouse inoculation test and cell culture technique for a diagnosis of rabies. With peroxidase anti-peroxidase technique the rabies specific reaction comprised inclusion bodies and a granular staining of the cytoplasm of affected cells. A specific positive reaction was found only in neurons, in which perikaryon as well as cell processes were affected. Fluorescent antibody test and peroxidase anti-peroxidase technique detected 98% each, mouse inoculation test 95% and cell culture technique 81% of the rabies positive animals. In conclusion, peroxidase anti-peroxidase technique allows a highly reliable diagnosis of rabies when only formalin-fixed and paraffin-embedded material is available. Histopathological alterations comprising Negri bodies, inflammatory and degenerative lesions were encountered in 53% of the rabies positive brains.
Zimmer
K
K
Institut für Veterinär-Pathologie der Justus-Liebig-Universität Giessen.
Wiegand
D
D
Manz
D
D
Frost
J W
JW
Reinacher
M
M
Frese
K
K
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D001921:Brain / Q000382:microbiology* / Q000473:pathology; D005069:Evaluation Studies as Topic; D007124:Immunoenzyme Techniques; D008322:Mammals; D011818:Rabies / Q000175:diagnosis / Q000662:veterinary*; D011820:Rabies virus / Q000276:immunology*
D000956:Antigens, Viral