Differential expression and distinct structure of 69- and 100-kDa forms of 2-5A synthetase in human cells treated with interferon.
Using specific monoclonal and polyclonal antibodies, the induction, synthesis and subcellular localization of 69- and 100-kDa forms of 2',5'-oligoadenylate (2-5A) synthetase (p69 and p100) were investigated in alpha-interferon-treated human HeLa and Daudi cells. Although both p69 and p100 were induced by interferon, there were significant differences in the interferon dose-response and the kinetics of synthesis of each protein in these cell lines. Both proteins are localized mainly in the cytoplasm. However, immunoenzymatic staining analysis of interferon-treated cells indicated that a proportion of p69 is concentrated around the nuclei and the rest is distributed in a specific pattern in the cytoplasm whereas p100 is found in a diffuse state in the cytoplasm. In accord with its association to cell membranes, p69 is myristilated. The [35S]cysteine and [3H]myristate-labeled p69 preparations were resolved by two-dimensional gel isoelectric focusing with pI values in the pH range of 7.0-8.0 and 7.0-7.5, respectively. These observations suggested the existence of two forms of p69 which might be complexed together as a dimer. In favor with a dimeric form of p69, in gel filtration experiments the peak of p69 was routinely found in fractions corresponding to a molecular mass of 160 kDa whereas p100 was recovered as a monomer. Taken together, these results indicate that p69 and p100 have distinct characteristics and that their expression is a specific property of each cell type in response to interferon.
Unité d'Oncologie Virale, UA Centre National de la Recherche Scientifique 1157, Institut Pasteur, Paris, France.
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Marié
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Svab
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Robert
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Galabru
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AG
Hovanessian
D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis* / Q000737:chemistry; D002451:Cell Compartmentation; D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007370:Interferon Type I / Q000494:pharmacology*; D007526:Isoelectric Point; D046911:Macromolecular Substances; D008970:Molecular Weight; D009226:Myristates / Q000378:metabolism; D011499:Protein Processing, Post-Translational
D007370:Interferon Type I; D046911:Macromolecular Substances; D009226:Myristates; D015088:2',5'-Oligoadenylate Synthetase