Evidence that there are only two tRNA(Phe) genes in Escherichia coli.
pheV, one of the genes that code for tRNA(Phe), was deleted from the chromosome of a strain of Escherichia coli K-12. As a consequence of this mutation, expression of pheA, the gene for chorismate mutase P-prephenate dehydratase, the first enzyme in the terminal pathway of phenylalanine biosynthesis, was derepressed. Similar derepression of pheA has been reported in pheR mutants of E. coli K-12 (J. Gowrishankar and J. Pittard, J. Bacteriol. 150:1130-1137, 1982). Attempts to introduce a pheR mutation into the delta pheV strain failed under circumstances suggesting that this combination of mutations is lethal. Southern blot analysis of pheV+ and delta pheV strains indicated that there are only two tRNA(Phe) genes in E. coli. It is recommended that the names pheU and pheV be retained for these genes.
Department of Microbiology, University of Melbourne, Parkville, Victoria Australia.
J
J
Pittard
J
J
Praszkier
A
A
Certoma
G
G
Eggertsson
J
J
Gowrishankar
G
G
Narasaiah
M J
MJ
Whipp
D015139:Blotting, Southern; D002872:Chromosome Deletion; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005838:Genotype; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D012360:RNA, Transfer, Phe / Q000235:genetics*; D015183:Restriction Mapping
D004269:DNA, Bacterial; D012360:RNA, Transfer, Phe