Restricted expression of the erythroid/brain glucose transporter isoform to perivenous hepatocytes in rats. Modulation by glucose.
The "erythroid/brain" glucose transporter (GT) isoform is expressed only in a subset of hepatocytes, those forming the first row around the terminal hepatic venules, while the "liver" GT is expressed in all hepatocytes. After 3 d of starvation, a three- to fourfold elevation of expression of the erythroid/brain GT mRNA and protein is detected in the liver as a whole; this correlates with the expression of this GT in more hepatocytes, those forming the first three to four rows around the hepatic venules. Starvation-dependent expression of the erythroid/brain GT on the plasma membrane of these additional hepatocytes is lost within 3 h of glucose refeeding; however, by immunoblotting we show that the protein is still present. Its loss from the surface is possibly explained by internalization.
Whitehead Institute For Biomedical Research, Cambridge, Massachusetts 02142.
M
M
Tal
D L
DL
Schneider
B
B
Thorens
H F
HF
Lodish
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D005947:Glucose / Q000494:pharmacology; D008099:Liver / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000145:classification / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D013217:Starvation; D013997:Time Factors; D014018:Tissue Distribution
D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; D005947:Glucose