Acidic fibroblast growth factor distribution in normal human eye and possible implications in ocular pathogenesis.
Using a rabbit anti-acidic fibroblast growth factor (anti-aFGF) antiserum, we tried to establish a precise mapping of aFGF localization in normal human ocular structures, from samples obtained by autopsies. Cell cultures of retinal pigment epithelium and ciliary pigment epithelium were also established and immunofluorescence studies were performed after 1 month. Corneal and conjunctival epithelia were strongly positive for anti-aFGF antibodies as well as the subcapsular epithelium of the lens. The cortical fibers were weakly reactive and the lens nucleus negative. A strong intracytoplasmic reactivity was observed in the pigmented and nonpigmented epithelial cells of ciliary processes and pars plana, both ex vivo and in vitro. Retina was brightly positive, mostly in the photoreceptor and plexiform layers. The possible involvement of aFGF in normal eye growth and in various ocular diseases was then discussed.
Department of Ophthalmology, Saint-Roch Hospital, Nice, France.
C
C
Baudouin
D
D
Fredj-Reygrobellet
J P
JP
Caruelle
D
D
Barritault
P
P
Gastaud
P
P
Lapalus
D000328:Adult; D000368:Aged; D002478:Cells, Cultured; D002924:Ciliary Body / Q000378:metabolism; D005123:Eye / Q000378:metabolism*; D005128:Eye Diseases / Q000209:etiology* / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008875:Middle Aged; D010857:Pigment Epithelium of Eye / Q000378:metabolism; D012163:Retinal Detachment / Q000378:metabolism; D014018:Tissue Distribution
D005346:Fibroblast Growth Factors