S-100B but not NGF, EGF, insulin or calmodulin is a CNS serotonergic growth factor.
The effects of S-100B, nerve growth factor (NGF), epidermal growth factor (EGF) and insulin were tested in cultured mesencephalic neurons. Only chronic S-100B showed enhancement (maximal at 3.2 ng/ml is 171%) after 3 days of incubation of the [3H]5-HT uptake capacity by serotonergic neurons. A single application at initial plating of S-100B (maximal at 5 ng/ml is 185%), but not calmodulin, increased the development of the [3H]5-HT uptake capacity by the cultured serotonergic neurons. Morphometric analysis of cultured 5-HT immunoreactive (IR) neurons showed an increase (135 and 147%) in neurite length 30 h after S-100B application of 16 and 3.2 ng/ml (respectively). These results suggest that S-100B is a serotonergic growth factor in the mammalian brain.
Azmitia
E C
EC
Department of Biology, New York University, NY 10003.
Dolan
K
K
Whitaker-Azmitia
P M
PM
D000818:Animals; D002147:Calmodulin / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D007150:Immunohistochemistry; D007328:Insulin / Q000494:pharmacology; D008636:Mesencephalon / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D009414:Nerve Growth Factors / Q000494:pharmacology*; D051381:Rats; D009418:S100 Proteins / Q000494:pharmacology*; D012701:Serotonin / Q000493:pharmacokinetics / Q000502:physiology*
D002147:Calmodulin; D007328:Insulin; D009414:Nerve Growth Factors; D009418:S100 Proteins; D012701:Serotonin