Early induction of anti-double-stranded DNA antibodies in lupus-prone MRL mice inoculated with Ly-24+ cells cloned from lymph node cells of an MRL/Mp-lpr/lpr mouse: possible effect of putative cytokines produced by cloned Ly-24+ cells.
We have reported that a cell line, KML1, obtained from lymph node cells of a 16-week-old female MRL/Mp-lpr/lpr (MRL/1) mouse produces a soluble factor(s) that promotes formation of anti-single-stranded (ss) DNA antibodies by cultures of spleen cells from old MRL/l mice (greater than 16 weeks old). Detailed examination revealed that KML1-7 cells cloned from the cell line KML1 were only positive for the Lys-24 (Pgp-1) phenotype, and were negative for other typical phenotypes tested, such as Thy-1, Ly-1, Ly-2, Ly-4 (L3T4) and Ly-5 (B220). This finding suggested that this line was of prothymocyte or myeloid cell lineage. From day 70 after subcutaneous inoculation of KML1-7 cells into 10-week-old MRL/Mp(-)+/+(MRL/n) mice, IgG anti-dsDNA antibodies began to replace IgM anti-dsDNA antibodies in the serum of these mice, but anticardiolipin, anti-trinitrophenyl hapten (TNP) and anti-poly(ADP-ribose) antibodies did not increase significantly. The predominant subclasses of anti-dsDNA antibodies that increased were IgG2a and IgG2b. IgG anti-dsDNA antibodies also became detectable as early as day 22 after inoculation of KML1-7 cells into MRL/l mice of 10 weeks old. The finding that in MRL/n mice the IgM isotype was increased first and was soon replaced by the IgG isotype was consistent with findings when spleen cells from MRL/n mice of 20 weeks old primed with ssDNA were cultured in the presence of conditioned medium containing a putative cytokine(s) (PC) produced by KML1-7 cells.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Molecular Oncology, University of Tokyo, Japan.
Y
Y
Kanai
S
S
Isonishi
T
T
Katagiri
T
T
Koizumi
K
K
Miura
Y
Y
Kurosawa
D000818:Animals; D000974:Antibodies, Antinuclear / Q000096:biosynthesis / Q000276:immunology*; D000954:Antigens, Surface / Q000008:administration & dosage / Q000096:biosynthesis / Q000276:immunology*; D001685:Biological Factors / Q000502:physiology*; D002460:Cell Line; D002999:Clone Cells; D016207:Cytokines; D004247:DNA / Q000276:immunology*; D005260:Female; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains; D010641:Phenotype; D016201:Receptors, Lymphocyte Homing; D013154:Spleen / Q000276:immunology
D000974:Antibodies, Antinuclear; D000954:Antigens, Surface; D001685:Biological Factors; D016207:Cytokines; D007074:Immunoglobulin G; D016201:Receptors, Lymphocyte Homing; D004247:DNA