Primary structure and functional expression of rat and human stem cell factor DNAs.
Partial cDNA and genomic clones of rat stem cell factor (SCF) have been isolated. Using probes based on the rat sequence, partial and full-length cDNA and genomic clones of human SCF have been isolated. Based on the primary structure of the 164 amino acid protein purified from BRL-3A cells, truncated forms of the rat and human proteins have been expressed in E. coli and mammalian cells and have been shown to possess biological activity. SCF is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow cultures. SCF exhibits potent synergistic activities in conjunction with colony-stimulating factors, resulting in increased colony numbers and colony size.
Martin
F H
FH
AMGEN Inc., AMGEN Center, Thousand Oaks, California 91320.
Suggs
S V
SV
Langley
K E
KE
Lu
H S
HS
Ting
J
J
Okino
K H
KH
Morris
C F
CF
McNiece
I K
IK
Jacobsen
F W
FW
Mendiaz
E A
EA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015870:Gene Expression; D005796:Genes; D015698:Genomic Library; D006133:Growth Substances / Q000494:pharmacology; D016298:Hematopoietic Cell Growth Factors / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010957:Plasmids; D011487:Protein Conformation; D051381:Rats; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000494:pharmacology; D012689:Sequence Homology, Nucleic Acid
D006133:Growth Substances; D016298:Hematopoietic Cell Growth Factors; D015345:Oligonucleotide Probes; D011994:Recombinant Proteins; D004247:DNA