Dietary fish oil intake: effects on glomerular prostanoid formation, hemodynamics, and proteinuria in nephrotoxic serum nephritis.
Dietary fish oil intake improves glomerular pathology and proteinuria in murine models of autoimmune disease. We evaluated glomerular prostanoid formation, glomerular hemodynamics, and proteinuria in rats with nephrotoxic serum nephritis (NSN) to test whether this beneficial effect of marine lipids also applies to other animal models of glomerular immune injury. Rats were fed diets (8 weeks) containing either cod liver oil or sunflower oil. NSN was induced with a rabbit anti-rat glomerular basement membrane antiserum. Antibody injection significantly stimulated glomerular thromboxane B2 (TxB2) formation in animals fed cod liver oil and sunflower oil at 2 hours, 24 hours, and 7 days. TxB2 production in glomeruli of sunflower oil rats, however, was five to seven times higher when compared with that in rats fed cod liver oil. The dietary regimen led to a significant decrease of glomerular TxB2 and prostaglandin E2 formation in the animals receiving cod liver oil when compared with those fed sunflower oil. Induction of NSN resulted in a significant fall of inulin clearance (Cin) and paraaminohippurate clearance at 2 hours, 24 hours, and 7 days in both groups. The decrease in Cin at 2 hours was greater in rats fed cod liver oil when compared with animals receiving sunflower oil (p less than 0.02); it was not different, however, at 24 hours and 7 days. Animals with NSN developed proteinuria. There was no difference in protein excretion between rats fed cod liver oil or those fed sunflower oil (days 2 and 7).(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Medicine, Johann Wolfgang Goethe University, Frankfurt, West Germany.
F
F
Thaiss
W
W
Schoeppe
P
P
Germann
R A
RA
Stahl
D000818:Animals; D004032:Diet; D015232:Dinoprostone / Q000378:metabolism; D015777:Eicosanoids / Q000378:metabolism; D005395:Fish Oils / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D005919:Glomerular Filtration Rate / Q000187:drug effects / Q000502:physiology; D006439:Hemodynamics / Q000187:drug effects / Q000502:physiology; D007106:Immune Sera / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism / Q000493:pharmacokinetics; D007678:Kidney Glomerulus / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D015289:Leukotrienes / Q000378:metabolism; D008657:Metabolic Clearance Rate / Q000187:drug effects; D009393:Nephritis / Q000097:blood; D011507:Proteinuria / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D013929:Thromboxane B2 / Q000378:metabolism; D010130:p-Aminohippuric Acid / Q000378:metabolism / Q000493:pharmacokinetics
D015777:Eicosanoids; D005395:Fish Oils; D007106:Immune Sera; D007328:Insulin; D015289:Leukotrienes; D013929:Thromboxane B2; D015232:Dinoprostone; D010130:p-Aminohippuric Acid