[Immobilization of myocardial tropomyosin].
Immobilized granulated myocardial tropomyosin with magnetic properties of prolonged period of storage was obtained. Tropomyosin inclusion into granules emulsion polymerisation is 89.5%. The largest amount of protein is extracted from granules after 2-3 days, and later this process is slowed down. Studying temperature effect, the maximum protein output was also shown to be reached after 2-3 days and depends on the temperature. Processing of granules, containing tropomyosin, with a solution with high concentration of hydrogen ions, the same results were obtained as under the influence of temperature. The use of 3 mol potassium rodanide did not show the peak of protein washing out of granules.
I P
IP
Gontar'
G F
GF
Sycheva
V P
VP
Podzolkov
O I
OI
Emel'ianova
M V
MV
Nikitin
I A
IA
Zborovskaia
D000941:Antigens; D001688:Biological Products / Q000302:isolation & purification* / Q000494:pharmacology; D004355:Drug Stability; D004356:Drug Storage; D006801:Humans; D007163:Immunosorbent Techniques; D007164:Immunosorbents; D009206:Myocardium; D012216:Rheumatic Diseases / Q000175:diagnosis / Q000276:immunology; D013696:Temperature; D013997:Time Factors; D014335:Tropomyosin / Q000302:isolation & purification* / Q000494:pharmacology
D000941:Antigens; D001688:Biological Products; D007164:Immunosorbents; D014335:Tropomyosin
Immobilizatsiia miokardial'nogo tropomiozina.