Receptor-active glycolipids of epithelial cells of the small intestine of young and adult pigs in relation to susceptibility to infection with Escherichia coli K99.
Glycolipids from mucosa scrapings of small intestine of neonatal and adult pigs were tested by the thin-layer chromatogram overlay assay for the binding of Escherichia coli K99. There was practically no binding to acid or non-acid glycolipids of adult pig, known to be resistant to infection with this bacterium. However, piglets, which are susceptible to infection, showed a clear binding to a doublet band in the acid glycolipid fraction. The receptor-active glycolipid was isolated and shown by mass spectrometry, NMR spectroscopy and degradation methods to be NeuGc alpha-3Gal beta 4Glc beta Cer (NeuGc-GM3), the two bands being due to heterogeneity of the ceramide. When tested against various reference glycolipids, NeuAc-GM3 was shown to be inactive. This ganglioside was dominating in adult pig. The apparent developmental disappearance of N-glycolyl groups in glycolipids of intestinal mucosa may have a correspondence in protein-linked sequences as well as thus explain the resistance of adult pigs to infection with E. coli K99.
Department of Medical Biochemistry, University of Göteborg, Sweden.
S
S
Teneberg
P
P
Willemsen
F K
FK
de Graaf
K A
KA
Karlsson
D000375:Aging; D000818:Animals; D001422:Bacterial Adhesion; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer; D004198:Disease Susceptibility; D004927:Escherichia coli Infections / Q000503:physiopathology*; D005732:Gangliosides / Q000302:isolation & purification / Q000502:physiology; D006017:Glycolipids / Q000302:isolation & purification / Q000502:physiology*; D006028:Glycosphingolipids / Q000502:physiology; D006801:Humans; D007413:Intestinal Mucosa / Q000254:growth & development / Q000382:microbiology*; D007421:Intestine, Small / Q000254:growth & development / Q000382:microbiology; D013058:Mass Spectrometry; D008958:Models, Molecular; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D013552:Swine
D005732:Gangliosides; D006017:Glycolipids; D006028:Glycosphingolipids