Failure of aerosolized endothelin (ET-1) to induce bronchial hyperreactivity in the guinea pig.
Aerosol administration of endothelin (ET-1) has been shown to provoke a potent bronchoconstriction in the guinea pig. We investigated whether or not, aerosolized ET-1 induces a bronchial hyperreactivity in the guinea pig. Aerosolized ET-1 (10 micrograms/ml for 60 min) did not alter the dose-response curve, established by successive aerosol administration of acetylcholine (ACh) 3-4 h and 18-24 h after challenge with the peptide. In a second protocol, aerosolized ET-1 (10 micrograms/ml for 3 min) induced, in anaesthetized guinea pigs a bronchopulmonary response but did not alter the dose-response curve to aerosolized ACh established 30 min after the challenge. These results suggest that ET-1 may participate to the early, but not the late alteration of the bronchopulmonary tone observed during pathophysiological conditions.
Institut Henri Beaufour, Les Ulis, France.
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V
Lagente
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Boichot
J
J
Mencia-Huerta
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P
Braquet
D000109:Acetylcholine / Q000008:administration & dosage / Q000494:pharmacology; D000280:Administration, Inhalation; D000336:Aerosols; D000818:Animals; D001980:Bronchi / Q000187:drug effects*; D001982:Bronchial Diseases / Q000139:chemically induced*; D004305:Dose-Response Relationship, Drug; D016232:Endothelins; D006168:Guinea Pigs; D008297:Male; D010455:Peptides / Q000008:administration & dosage / Q000494:pharmacology*
D000336:Aerosols; D016232:Endothelins; D010455:Peptides; D000109:Acetylcholine