Accelerating effect of glutathione on hydroxylation of phenylalanine by stimulated polymorphonuclear leukocytes.
Sulfhydryl compounds significantly accelerated the hydroxylation of phenylalanine by stimulated polymorphonuclear leukocytes. The reduced form of glutathione (G-SH) was most effective. The hydroxylation reaction in the presence of G-SH was largely prevented by superoxide dismutase and hydroxyl radical scavengers. The results suggest that a much faster production of hydroxyl radical may occur in a reaction mixture containing both G-SH and stimulated polymorphonuclear leukocytes than in that containing stimulated polymorphonuclear leukocytes alone.
Laboratory of Biochemistry, Kyoto Pharmaceutical University, Japan.
S
S
Fujimoto
S
S
Ishimitsu
H
H
Kanazawa
A
A
Ohara
T
T
Hayakawa
D000818:Animals; D005260:Female; D005978:Glutathione / Q000494:pharmacology*; D006168:Guinea Pigs; D006900:Hydroxylation; D066298:In Vitro Techniques; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism*
D010649:Phenylalanine; D005978:Glutathione