Divergency in the specificity of the induction and maintenance of neonatal suppression.
Neonatal treatment of BALB/c mice with anti-VHT15 antibodies suppresses serum expression of VHT15 immunoglobulins in adult animals (2 months) which remains for over 8 months in half of the cases. Suppressed mice, however, contain control numbers of B cells expressing genes of the S107VH family and producing VHT15 after mitogenic stimulation. Furthermore, immunization with phosphorylcholine (PC) breaks suppression and stimulates the production of VHT15 anti-PC antibodies. These animals, however, contain no detectable B lymphocytes expressing the T15 idiotype and produce no T15 idiotype-positive antibodies in response to PC. These results are discussed in the context of lymphocyte repertoire selection.
Département d'Immunologie, Institut Pasteur, Paris, France.
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Mota-Santos
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Masmoudi
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Voegtlé
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Freitas
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Coutinho
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Cazenave
D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001402:B-Lymphocytes / Q000276:immunology; D015342:DNA Probes; D015870:Gene Expression; D007108:Immune Tolerance / Q000187:drug effects; D007114:Immunization; D007135:Immunoglobulin Variable Region / Q000032:analysis; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D010767:Phosphorylcholine / Q000494:pharmacology
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D015342:DNA Probes; D007135:Immunoglobulin Variable Region; D010767:Phosphorylcholine