A malaria heat-shock-like determinant expressed on the infected hepatocyte surface is the target of antibody-dependent cell-mediated cytotoxic mechanisms by nonparenchymal liver cells.
Cultured hepatic stages of Plasmodium falciparum and P. yoelii and with a monoclonal antibody recognizing a C-terminal fragment of the P. falciparum heat-shock-like protein (Pfhsp70) revealed that synthesis of this antigen first occurs during intrahepatic development of the parasite, at the two nuclei stage. Using a variety of techniques, including scanning electron microscopy, we observed that this antigenic determinant was expressed on the infected hepatocyte membrane. Its participation in antibody-dependent cell-mediated cytotoxicity was investigated. While no effect was obtained with peripheral blood cells, we found that 25% of the schizonts were specifically lysed when using spleen cells at a killer/target ratio of 30/1. More interestingly, with nonparenchymal liver cells, up to 50% of the hepatic parasites disappeared with a killer/target ratio of 10/1.
Rénia
L
L
INSERM U 313, Groupe Hospitalier Pitié-Salpêtrière, Paris, France.
Mattei
D
D
Goma
J
J
Pied
S
S
Dubois
P
P
Miltgen
F
F
Nüssler
A
A
Matile
H
H
Menégaux
F
F
Gentilini
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D000920:Antibody-Dependent Cell Cytotoxicity / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008099:Liver / Q000276:immunology* / Q000469:parasitology; D008288:Malaria / Q000276:immunology*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010963:Plasmodium falciparum / Q000276:immunology; D010967:Plasmodium yoelii / Q000276:immunology; D012377:Rodentia
D000911:Antibodies, Monoclonal; D006360:Heat-Shock Proteins