Regulation of proliferation of embryonic heart mesenchyme: role of transforming growth factor-beta 1 and the interstitial matrix.
Proliferation of atrioventricular cushion mesenchyme of the embryonic avian heart maintained in three-dimensional aggregate culture is stimulated by interaction with the interstitial matrix. Chicken serum or transforming growth factor-beta 1, which stimulates proliferation, induces matrix deposition in regions of the aggregate showing high labeling indices with tritiated thymidine. Dispersed heart mesenchyme interstitial matrix introduced into serum-free culture is incorporated into the aggregate and stimulates cellular proliferation similar to serum or transforming growth factor-beta 1. Proliferation is reversibly inhibited by the peptide Gly-Arg-Gly-Asp-Ser-Pro. It is suggested that transforming growth factor-beta 1 stimulates the production of interstitial matrix and that a sufficient stimulus for proliferation in this system is the presence of the matrix, which acts as the adhesive support for cellular anchorage.
Department of Pediatrics, University of California Davis Medical Center, Sacramento 95817.
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Choy
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Armstrong
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Armstrong
D000818:Animals; D001345:Autoradiography; D002449:Cell Aggregation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D003238:Connective Tissue / Q000187:drug effects / Q000196:embryology*; D003239:Connective Tissue Cells; D004261:DNA Replication / Q000187:drug effects; D005109:Extracellular Matrix / Q000502:physiology*; D006321:Heart / Q000196:embryology*; D013936:Thymidine / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014316:Tritium
D016212:Transforming Growth Factor beta; D014316:Tritium; D013936:Thymidine