An in vivo replica method for the site-specific detection of Candida albicans on the denture surface in denture stomatitis patients: correlation with clinical disease.
A site-specific agar replica technique for detecting Candida albicans on the acrylic resin denture surface of denture stomatitis patients has been developed. The method is selective for C. albicans during a finite incubation period with a specific synthetic growth medium. C. albicans colonies can be geographically observed on the replica and their presence can be correlated with inflammatory lesions visible on the mucosa of the maxillary and mandibular residual ridges. In 12 denture stomatitis patients studied, a close clinical correlation of Newton type III patients was noted but this clinical correlation could not be observed in Newton type I and II patients. In general, the number of C. albicans colonies increased with the severity of the inflammation. The findings are discussed in light of lack of knowledge of the etiology of the stomatitis. The importance of the replica method is also discussed.
State University of New York, School of Dental Medicine, Stony Brook.
R P
RP
Santarpia
J J
JJ
Pollock
R P
RP
Renner
E
E
Spiechowicz
D000362:Agar; D002176:Candida albicans / Q000302:isolation & purification*; D015169:Colony Count, Microbial; D005260:Female; D006801:Humans; D008297:Male; D009172:Mycology / Q000379:methods; D013280:Stomatitis / Q000382:microbiology*; D013282:Stomatitis, Denture / Q000382:microbiology* / Q000473:pathology
D000362:Agar