How cells recognize damaged DNA: clues from xeroderma pigmentosum and yeast.
Xeroderma pigmentosum (XP) is characterized by the defective excision repair of DNA damaged by many agents, including ultraviolet radiation (UV) and cisplatin. We have identified a factor in human cells that recognizes multiple forms of DNA damage and is absent in XP complementation group E. Denoted XPE binding factor, it is expressed at five-fold higher levels in tumor cell lines resistant to the antitumor drug cisplatin. Finally, although it does not have photoreactivating activity, XPE binding factor shares multiple binding characteristics with yeast photolyase, suggesting that it is the human homolog of photolyase.
Chu
G
G
Department of Medicine, Stanford University School of Medicine, CA 94305.
Chang
E
E
Patterson
M
M
D002478:Cells, Cultured; D002945:Cisplatin / Q000494:pharmacology; D004247:DNA / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000235:genetics; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000502:physiology; D005656:Fungal Proteins / Q000378:metabolism; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014983:Xeroderma Pigmentosum / Q000235:genetics* / Q000473:pathology
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D004247:DNA; D004255:Deoxyribodipyrimidine Photo-Lyase; D002945:Cisplatin