Effects of isoxazolyl-naphthoquinoneimines on growth and oxygen radical production in Trypanosoma cruzi and Crithidia fasciculata.
Several 4-(aminomethylisoxazolyl)-1,2-naphthoquinones inhibited growth and DNA synthesis in Trypanosoma cruzi and stimulated O2 uptake and O2-. generation by the parasite epimastigotes and their mitochondrial and microsomal membranes; these results support the idea that oxygen radicals play a role in quinone toxicity. Maximal effects on respiration and O2-. generation were observed with antimycin-inhibited cells. Similar results as well as stimulation of H2O2 production were obtained with Crithidia fasciculata despite the presence of catalase in this organism.
Bioenergetics Research Centre, School of Medicine, University of Buenos Aires, Argentina.
M N
MN
de Tarlovsky
S G
SG
Goijman
M P
MP
Molina Portela
A O
AO
Stoppani
D000818:Animals; D000981:Antiprotozoal Agents; D003421:Crithidia / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000378:metabolism; D007097:Imines / Q000494:pharmacology*; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism; D007555:Isoxazoles / Q000494:pharmacology*; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009285:Naphthoquinones / Q000494:pharmacology*; D010080:Oxazoles / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism*; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
D000981:Antiprotozoal Agents; D005609:Free Radicals; D007097:Imines; D007555:Isoxazoles; D009285:Naphthoquinones; D010080:Oxazoles; D004247:DNA; D006861:Hydrogen Peroxide; D010100:Oxygen