Continuous growth of Candida utilis under periodic change of growth-limiting substrate.
A periodic change of limitation by glucose and ammonia was effected during continuous cultivation of Candida utilis. Values of feed parameters providing periodic nitrogen limitation were established. Cell biomass yield, macromolecular composition and parameters of individual cells (cell mass, budding percentage and cell-wall polysaccharide per surface square unit) were examined. The cyclic regime was found to result in culture synchrony. Parameters obtained on the basis of cell counts displayed a high sensitivity to changes in limitation where as the remaining parameters were less sensible.
Institute of Biochemistry and Physiology of Microorganisms, USSR Academy of Sciences, Puschino.
I G
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Minkevich
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M
Sobotka
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D
Vraná
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I
Havlík
D000641:Ammonia / Q000378:metabolism*; D002175:Candida / Q000032:analysis / Q000254:growth & development* / Q000378:metabolism; D002453:Cell Cycle; D003470:Culture Media; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D009584:Nitrogen / Q000378:metabolism; D011134:Polysaccharides / Q000032:analysis
D003470:Culture Media; D011134:Polysaccharides; D000641:Ammonia; D005947:Glucose; D009584:Nitrogen