In vitro linoleic acid activation of protein kinase C.
The importance of membrane fluidity in the activation of protein kinase C (PKC) was examined using the membrane fluidizer, linoleic acid, in a well-defined model membrane system. Biochemical and biophysical properties of the system were monitored. Linoleic acid activated PKC to a level of 50% of that observed for diacylglycerol. In contrast, linoleic acid did not directly interact with the phorbol ester binding site as did diacylglycerol. This was determined by the lack of involvement of the ionizable group of the fatty acid with activity and the enhancement of phorbol ester binding by linoleic acid and its ester analogs. The membrane fluidity of this model membrane system in the presence of linoleic acid was increased as determined by fluorescence polarization. This increased the availability of phospholipids, thus, explaining the linoleic acid-induced enhancement of phorbol ester binding. The PKC conformation as determined from intrinsic tryptophan fluorescence spectra was different for lipid mixtures containing linoleic acid or diacylglycerol correlating with the difference in biochemical activation properties. This study provides evidence that membrane fluidization is not the predominant function of the lipid activator in PKC activation, but may play a role in obtaining the preferred membrane state for maximal activation.
Lester
D S
DS
Department of Membrane Research, Weizmann Institute of Science, Rehovot, Israel.
D000818:Animals; D001921:Brain / Q000201:enzymology; D004075:Diglycerides / Q000494:pharmacology; D004789:Enzyme Activation; D005454:Fluorescence Polarization; D006657:Histones / Q000378:metabolism; D066298:In Vitro Techniques; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology*; D008081:Liposomes; D008297:Male; D008560:Membrane Fluidity; D011493:Protein Kinase C / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014364:Tryptophan / Q000737:chemistry
D004075:Diglycerides; D006657:Histones; D008041:Linoleic Acids; D008081:Liposomes; D014364:Tryptophan; D019787:Linoleic Acid; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate