Expression of simian virus 40 large T antigen in Escherichia coli using vectors based on the regulatable rac promoter.
Simian Virus 40 large T antigen is a multi-functional protein that is involved in the initiation of viral DNA replication, regulation of viral transcription and cell transformation. Bacterial expression vectors, pER23-1 and pER23-2, that are based on the regulatable rac promoter were used to produce T antigen either as a free protein or as a fusion protein. We have observed efficient transcription of the cloned T antigen gene in most of the recombinants. However, expression of the T antigen protein was inefficient and most of the expressed protein was truncated. This may be due to differences in codon usage in E. coli or to rapid protein degradation.
Department of Biochemistry, University of Nebraska Medical Center, Omaha 68198-4525.
J M
JM
Pistillo
J K
JK
Vishwanatha
D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D003062:Codon; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics
D000952:Antigens, Polyomavirus Transforming; D003062:Codon; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins