The role of receptor kinase in insulin action and the effects of insulin on human hepatoma cells.
Insulin has both short- and long-term effects on cellular metabolism. The short-term effects are known to involve the insulin receptor, a protein kinase capable of phosphorylating itself and other proteins. The role of the receptor was elucidated by studies of a mutant insulin receptor which lacked kinase activity and inhibited several actions of insulin. The long-term effects of insulin could be demonstrated by its growth-promoting effect on hepatoma cells, and by the suppression in transfected hepatoma cells of hepatitis B virus antigen production in a dose-dependent manner. The process whereby insulin appears to regulate gene expression is not clearly understood.
Department of Medical Research, Veterans General Hospital, Taipei, Taiwan, China.
C K
CK
Chou
D002455:Cell Division / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006514:Hepatitis B Surface Antigens; D006515:Hepatitis B virus / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007328:Insulin / Q000378:metabolism / Q000494:pharmacology*; D009154:Mutation; D011494:Protein Kinases / Q000378:metabolism*; D011972:Receptor, Insulin / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects
D006514:Hepatitis B Surface Antigens; D007328:Insulin; D011494:Protein Kinases; D011972:Receptor, Insulin