Mechanism of action of atpenin B on Raji cells.
Atpenin B, a new antifungal antibiotic produced by Penicillium sp. FO-125, inhibited the growth of Raji cells (IC50, 30 microM). The incorporation of [14C]leucine and [3H]thymidine into Raji cells was inhibited by atpenin B with IC50 values of 0.10 and 0.12 microM, respectively. The incorporation of [14C]palmitate into the cells was not inhibited but its incorporation into lipid fractions was inhibited by atpenin B (IC50, 0.13-0.24 microM). Studies on the site of atpenin B action demonstrated that atpenin B decreases the cellular adenosine 5'-triphosphate (ATP) level in Raji cells with IC50 value of 0.020 microM, suggesting the inhibition of ATP-generating system by atpenin B.
Kitasato Institute, Kitasato University, Tokyo, Japan.
K
K
Oshino
H
H
Kumagai
H
H
Tomoda
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S
Omura
D000217:Acyltransferases / Q000187:drug effects; D000255:Adenosine Triphosphate / Q000378:metabolism; D000935:Antifungal Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003066:Coenzyme A Ligases / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008055:Lipids / Q000096:biosynthesis; D008862:Microsomes, Liver / Q000201:enzymology; D011728:Pyridones
D000935:Antifungal Agents; D008055:Lipids; D011728:Pyridones; C058279:atpenin B; D000255:Adenosine Triphosphate; D000217:Acyltransferases; D003066:Coenzyme A Ligases