High performance liquid chromatography determination of doxorubicin and daunorubicin in plasma using UV detection and column switching.
A method for the determination of doxorubicin and daunorubicin in plasma is described. The plasma is injected directly into a loop column and then washed with water. After switching the injection valve, the sample is separated on a phenyl column using detection at 254 nm. The detection limit is 10 ng/mL, the coefficient of variation is 7% for 100 ng/mL of doxorubicin and 4% for 200 ng/mL of daunorubicin.
JZD Agrokombinat, Slusovice, Czechoslovakia.
A
A
Mikan
J
J
Martinez Lanao
F
F
Gonzalez Lopez
A
A
Dominguez-Gil Hurle
D002851:Chromatography, High Pressure Liquid; D003630:Daunorubicin / Q000097:blood*; D004317:Doxorubicin / Q000097:blood*; D006801:Humans; D013056:Spectrophotometry, Ultraviolet
D004317:Doxorubicin; D003630:Daunorubicin