A compensatory mechanism improving red cell membrane fluidity in hemodialysed patients.
A fluorescence polarization technique with 1,6-diphenyl 1,3,5-hexatriene as a fluorescent probe was used to determine the fluidity of red cell membranes from hemodialysed patients before and after dialysis. After dialysis, there was a decrease in fluidity and activation energy values revealed a significantly changed distribution. The membrane lipid composition showed a significant increase in cholesterol after dialysis (p less than 0.001) and a significant reduction in saturated fatty acids (p less than 0.01) with an increase in unsaturated fatty acids (p less than 0.05). A compensatory mechanism could be suggested involving a reduction of saturated fatty acids in response to the increase in the cholesterol/phospholipid ratio. This could lead to an optimization of membrane fluidity.
Laboratoire de Biochimie Médicale A, Université de Bordeaux II, Clermont-Ferrand, France.
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Peuchant
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Motta
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Salles
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Clerc
D000328:Adult; D000368:Aged; D002784:Cholesterol / Q000097:blood; D004161:Diphenylhexatriene; D004910:Erythrocyte Membrane / Q000737:chemistry*; D005260:Female; D005454:Fluorescence Polarization; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000628:therapy; D008297:Male; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000097:blood*; D008875:Middle Aged; D010743:Phospholipids / Q000097:blood; D006435:Renal Dialysis; D014511:Uremia / Q000097:blood / Q000628:therapy
D008563:Membrane Lipids; D010743:Phospholipids; D004161:Diphenylhexatriene; D002784:Cholesterol