Evidence for endothelin-1 release from resistance vessels of rats in response to hypoxia.
To elucidate further the contribution of endothelin into endothelium-dependent vasoconstriction evoked by hypoxia, we observed endothelin release during hypoxia. Endothelin was detectable in perfusate from mesenteric artery. Immunoreactive endothelin was confirmed as endothelin-1 by a reverse phase-HPLC. Endothelin release increased 4.1 +/- 1.3 to 12.4 +/- 2.0 pg/30 min without changing perfusion pressure. Thirty minutes of hypoxia stimulated endothelin release by 71 +/- 11% (P less than 0.05) and was associated with an elevation of perfusion pressure. These results suggest that endothelin contributes to endothelium-dependent vasoconstriction by hypoxia in mesenteric artery and may play an important role in the local peripheral vascular tone.
Department of Geriatric Medicine, Osaka University Medical School, Japan.
H
H
Rakugi
Y
Y
Tabuchi
M
M
Nakamaru
M
M
Nagano
K
K
Higashimori
H
H
Mikami
T
T
Ogihara
N
N
Suzuki
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D016232:Endothelins; D000860:Hypoxia / Q000378:metabolism*; D008297:Male; D008638:Mesenteric Arteries / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D016232:Endothelins; D010455:Peptides