A two-dimensional gel protein database of noncultured total normal human epidermal keratinocytes: identification of proteins strongly up-regulated in psoriatic epidermis.
A two-dimensional (2-D) gel database of proteins from noncultured total normal human epidermal keratinocytes has been established. A total of 1449 [35S]methionine labelled proteins (1112 isoelectric focusing, 337 nonequilibrium pH gradient electrophoresis) were resolved and recorded using computer assisted (PDQ-SCAN and PDQUEST software) 2-D gel electrophoresis. By matching the protein patterns of total keratinocytes and transformed human amnion cells (master database; Celis et al., Leukemia 1988, 2, 561-602) as well as by 2-D immunoblotting and microsequencing of keratinocyte proteins, it was possible to identify 72 polypeptides in the keratinocyte database. The database also includes data on polypeptides that are synthesized at a higher level by keratinocytes enriched in basal cells, and on six secreted proteins which are produced, albeit at a reduced rate, by normal keratinocytes and that are strongly up-regulated in psoriatic epidermis (Celis et al., FEBS Letters, in press).
Celis
J E
JE
Institute of Medical Biochemistry, Aarhus University, Denmark.
Crüger
D
D
Kiil
J
J
Dejgaard
K
K
Lauridsen
J B
JB
Ratz
G P
GP
Basse
B
B
Celis
A
A
Rasmussen
H H
HH
Bauw
G
G
D015180:Electrophoresis, Gel, Two-Dimensional; D005455:Fluorescent Antibody Technique; D006801:Humans; D007256:Information Systems; D007525:Isoelectric Focusing; D015603:Keratinocytes / Q000378:metabolism*; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D011565:Psoriasis / Q000378:metabolism*; D012984:Software; D015854:Up-Regulation
D011506:Proteins