[Indirect spectrophotometric detection by reversed-phase liquid chromatography. Application in drug analysis].
By inclusion of a high U.V. or visible-absorbing component to the mobile phase of a RP-HPLC system, it is possible, after they were separated by chromatography, to detect indirectly and to quantify analytes which do not themselves absorb at the detection wavelength. The principles of this "indirect detection", as well as the parameters which can influence it were studied, and the method was applied to the qualitative and quantitative determination of a lot of compounds used as active substances or as technological adjuvants in drugs.
Herne
P
P
Université de Liège, Institut de Pharmacie.
D002851:Chromatography, High Pressure Liquid; D004364:Pharmaceutical Preparations / Q000032:analysis*; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet
D004364:Pharmaceutical Preparations
Détection spectrophotométrique indirecte en chromatographie liquide sur phases greffées. Applications à l'analyse des médicaments.