Cloning and structure of the pyrE gene of Lactobacillus plantarum CCM 1904.
The pyrE gene of Lactobacillus plantarum CCM 1904, coding for the orotate phosphoribosyl transferase involved in the pyrimidine biosynthetic pathway, was cloned in Escherichia coli and sequenced. The predicted polypeptide sequence extending over 212 amino acids (MW 22,690) was compared to those of E. coli and to those of lower eukaryotes (Saccharomyces cerevisiae, Podospora anserina, Sordaria macrospora, Dictyostelium discoideum). Important conserved stretches were revealed, implying that these proteins are closely related.
Bouia
A
A
Laboratoire de Microbiologie, Université Louis-Pasteur, Strasbourg, France.
Bringel
F
F
Frey
L
L
Belarbi
A
A
Guyonvarch
A
A
Kammerer
B
B
Hubert
J C
JC
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D007778:Lactobacillus / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D009962:Orotate Phosphoribosyltransferase / Q000235:genetics*; D010957:Plasmids; D012689:Sequence Homology, Nucleic Acid
D009962:Orotate Phosphoribosyltransferase