Genotypic analysis in large cell lymphomas expressing a restricted set of differentiation antigens.
Immunophenotyping and immunogenotyping were performed in a series of 8 large cell lymphomas exhibiting anaplastic or "histiocytic" morphology and displaying an uncertain phenotype due to a restricted number of differentiation antigens. 6 cases expressed the Ki-1 antigen. 4 cases expressed one or two B-cell markers and contained rearrangements of the immunoglobulin genes. One of them also exhibited a T-cell receptor (TCR) beta gene rearrangement. 3 cases expressed a single T-cell differentiation antigen. Among them, only 1 displayed both gamma and beta TCR gene rearrangement; 1 only contained a gamma TCR gene rearrangement and 1 completely lacked clonal rearrangements. The eight cases expressed an inconclusive immunophenotype due to an abundant population of reactive cells but showed an immunoglobulin gene rearrangement. In conclusion, 5 out of the 8 unusual lymphomas studied here could be characterized by immunogenotyping. This approach was, however, inconclusive in the 3 remaining cases, whose lineage and differentiation stage remain poorly defined.
Xerri
L
L
Laboratoire d'Anatomie Pathologique, Centre Anti-Cancéreux, Marseille, France.
Horschowski
N
N
Payan
M J
MJ
Hassoun
J
J
Horschowski
N
N
Carcassonne
Y
Y
Manonni
P
P
Chuchana
P
P
D000328:Adult; D000943:Antigens, Differentiation / Q000235:genetics / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology*; D015139:Blotting, Southern; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000276:immunology / Q000473:pathology; D003937:Diagnosis, Differential; D005838:Genotype; D006801:Humans; D008223:Lymphoma / Q000175:diagnosis / Q000276:immunology* / Q000473:pathology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000175:diagnosis / Q000276:immunology / Q000473:pathology; D008297:Male; D008875:Middle Aged
D000943:Antigens, Differentiation; D000951:Antigens, Neoplasm