Interleukin-1 stimulates glucose transport in rat adipose cells. Evidence for receptor discrimination between IL-1 beta and IL-1 alpha.
The effect of interleukin 1 (IL-1) on glucose transport activity in isolated rat adipose cells was examined. IL-1 beta stimulated 3-O-methylglucose (3OMG) transport in a time and dose dependent manner. This effect appears to be due to increased maximal transport velocity (Vmax) of the carrier. Addition of insulin and IL-1 beta resulted in an additive stimulation of transport, suggesting different mechanisms. IL-1 alpha had no effect on glucose transport. Glu-4, a relatively inactive IL-1 beta analogue in most cells, stimulated glucose uptake in a time and dose dependent manner with kinetics indistinguishable from those of IL-1 beta.
Department of Nutrition, Rutgers University, New Brunswick, NJ.
A
A
Garcia-Welsh
J S
JS
Schneiderman
D L
DL
Baly
D019325:3-O-Methylglucose; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology*; D007700:Kinetics; D046911:Macromolecular Substances; D008297:Male; D008757:Methylglucosides / Q000378:metabolism*; D008759:Methylglycosides / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D007375:Interleukin-1; D046911:Macromolecular Substances; D008757:Methylglucosides; D008759:Methylglycosides; D019325:3-O-Methylglucose