Blood lymphocyte subsets in ATG-treated and allografted rats.
A single dose of rabbit antithymocyte globulin (ATG) was given as the sole immunosuppressive therapy in a model of strong MHC barrier rat heart allotransplantation. PVG/c hearts transplanted to Wistar/Kyoto (WKy) rats resulted in long-term surviving (LTS) grafts and cell-mediated lympholysis (CML) unresponsiveness in 50% of the animals. The effects of ATG treatment on the peripheral blood lymphocyte subsets were studied by flow cytometry. The absolute T-lymphocyte levels decreased to less than 5% and were normalized after 2 weeks. CD8-positive cells were normalized within 1 week, whereas CD4- and CD5-positive cells remained low. Rats with LTS grafts had low levels of all T-lymphocyte markers, especially the CD4- and CD5-positive cells. Rats rejecting their grafts showed an eightfold increase in levels of CD8- and CD5-positive lymphocytes and a twofold increase in levels of CD4-expressing lymphocytes. It is concluded that ATG treatment causes the immediate elimination of large lymphoid populations as well as long-lasting immunomodulation detectable in peripheral blood.
Wramner
L
L
Department of Surgery, University of Göteborg, Sweden.
Robbins
D S
DS
Kjellsson
B
B
Mjörnstedt
L
L
Olausson
M
M
Brynger
H
H
Söderström
T
T
D000818:Animals; D000961:Antilymphocyte Serum / Q000627:therapeutic use*; D006085:Graft Survival; D016027:Heart Transplantation / Q000276:immunology*; D007111:Immunity, Cellular; D007958:Leukocyte Count; D016131:Lymphocyte Subsets / Q000276:immunology; D051381:Rats; D011921:Rats, Inbred WKY; D013601:T-Lymphocytes / Q000276:immunology*; D014184:Transplantation, Homologous
D000961:Antilymphocyte Serum