A strategy for founding a global lymphocyte proteinpaedia and gene catalogue.
In this article, Ivan Lefkovits, John Kettman and Christopher Coleclough describe a strategy for a comprehensive analysis of the genes active in lymphocytes. It allows direct access to the coding sequence of any gene, provided that the two-dimensional gel spot coordinate position is known. The authors have constructed representative lymphocyte cDNA libraries and partitioned them into many sectors, each containing a unique set of cDNA clones. Data from the analysis of natural lymphocyte proteins is compiled into a 'proteinpaedia', which is matched to a gene catalogue comprising the partitioned recombinant phage collection.
Basel Institute for Immunology, Switzerland.
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Lefkovits
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Kettman
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Coleclough
D000818:Animals; D001798:Blood Proteins / Q000032:analysis / Q000235:genetics; D004247:DNA; D015723:Gene Library; D006801:Humans; D007256:Information Systems; D008214:Lymphocytes / Q000032:analysis
D001798:Blood Proteins; D004247:DNA