Detection of interleukin-6 (IL-6) in human bone marrow myeloma cells by light and electron microscopy.
We used an Avidin-Biotin peroxidase complex (ABC) immunoperoxidase technique to evaluate the localization of IL-6 of human bone marrow cells in multiple myeloma (MM). The cellular distribution of IL-6 was determined at light and electron microscopic levels. The author's study indicated that cytoplasmic IL-6 was detected only in the myeloma cells of bone marrow cells. Immunoelectron microscopic (immuno-EM) study showed positive reactivity mainly in the perinuclear space (PNS), well-developed rough endoplasmic reticulum (RER), and Golgi area.
Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
K
K
Ohtake
T
T
Yano
K
K
Kameda
T
T
Ogawa
D001853:Bone Marrow / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D046508:Culture Techniques; D006801:Humans; D007158:Immunologic Techniques; D015850:Interleukin-6 / Q000378:metabolism*; D008854:Microscopy, Electron; D009101:Multiple Myeloma / Q000378:metabolism* / Q000473:pathology
D015850:Interleukin-6