Expression and characterization of TCA3: a murine inflammatory protein.
TCA3 is a cDNA originally isolated from activated T cells. Transcription of this gene has been shown to correlate with Ag-induced cellular activation of both T cells and mast cells. Based on the predicted amino acid sequence encoded by the cDNA, we previously proposed that TCA3 represents a cytokine. In this report we have used rDNA technology to express TCA3 in two mammalian cell lines. In both cases, TCA3 was expressed as a secreted molecule with an apparent molecular mass of 16 kDa. Digestion of the (rTCA3) with the enzyme N-glycanase revealed that approximately 8 kDa is caused by N-linked glycosylation. Intradermal injection of rTCA3 into mouse footpads resulted in a rapid swelling response. The sites of injection were characterized histologically by a local accumulation of neutrophils. These findings are discussed with particular attention to a family of related proteins, some of whose members also have inflammatory properties.
Department of Pathology, Harvard Medical School, Boston, MA 02115.
S D
SD
Wilson
V K
VK
Kuchroo
D I
DI
Israel
M E
ME
Dorf
D000818:Animals; D002478:Cells, Cultured; D054402:Chemokine CCL1; D019742:Chemokines, CC; D002630:Chemotactic Factors / Q000235:genetics*; D003001:Cloning, Molecular; D016207:Cytokines / Q000235:genetics*; D015870:Gene Expression; D006023:Glycoproteins / Q000235:genetics*; D006031:Glycosylation; D066298:In Vitro Techniques; D007249:Inflammation / Q000503:physiopathology; D051379:Mice; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational; D054401:Receptors, CCR8; D011994:Recombinant Proteins; D014162:Transfection
C065971:CCL1 protein, human; C489319:Ccr8 protein, mouse; D054402:Chemokine CCL1; D019742:Chemokines, CC; D002630:Chemotactic Factors; D016207:Cytokines; D006023:Glycoproteins; D054401:Receptors, CCR8; D011994:Recombinant Proteins