Cloning of the p50 DNA binding subunit of NF-kappa B: homology to rel and dorsal.
The DNA binding subunit of the transcription factor NF-kappa B, p50, has been cloned. p50 appears to be synthesized as a larger protein that is then processed to its functional size. Sequence analysis reveals remarkable homology for over 300 amino acids at the amino-terminal end to the oncogene v-rel, its cellular homolog c-rel, and the Drosophila maternal effect gene dorsal. This establishes NF-kappa B as a member of the rel family of proteins, all of which display nuclear-cytosolic translocation. Protein sequence from the p65 polypeptide has established that it is not encoded in the same mRNA as p50. However, p65 appears homologous to c-rel, suggesting that c-rel may form heterodimers with p50 and rel may include a homodimerization motif.
Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts 02142.
S
S
Ghosh
A M
AM
Gifford
L R
LR
Riviere
P
P
Tempst
G P
GP
Nolan
D
D
Baltimore
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000302:isolation & purification; D004330:Drosophila / Q000235:genetics*; D005260:Female; D005796:Genes; D006801:Humans; D008168:Lung / Q000378:metabolism; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D016328:NF-kappa B; D020572:Oncogene Proteins v-rel; D010446:Peptide Fragments / Q000302:isolation & purification; D014176:Protein Biosynthesis; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011817:Rabbits; D011994:Recombinant Proteins / Q000302:isolation & purification; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics* / Q000302:isolation & purification; D014158:Transcription, Genetic; D014357:Trypsin
D004268:DNA-Binding Proteins; D046911:Macromolecular Substances; D016328:NF-kappa B; D020572:Oncogene Proteins v-rel; D010446:Peptide Fragments; D011994:Recombinant Proteins; D015682:Retroviridae Proteins, Oncogenic; D014157:Transcription Factors; D004247:DNA; D011505:Protein-Tyrosine Kinases; D014357:Trypsin