Effects of heat and other agents on amino acid uptake in Escherichia coli.
In Escherichia coli K1060 grown at 37 degrees C we observed that the uptake of both L-[3H]leucine and L-[35S]methionine was inhibited by exposure of the cells to 48 degrees C. The calcium channel blockers diltiazem and verapamil, and the anti-arrhythmic agent quinidine, inhibited the uptake of L-[3H]leucine at both 37 degrees C and 48 degrees C. Verapamil also inhibited the uptake of L-[35S]methionine at 37 degrees C, but at 48 degrees C protected against some of the heat-induced decrease in the uptake of this amino acid. The local anaesthetic procaine markedly inhibited the uptake of both labelled amino acids at temperatures between 37 degrees C and 48 degrees C. Amino acid uptake and cell killing were not correlated.
Department of Radiation Oncology, Oregon Health Sciences University, Portland 97201.
R H
RH
Wainberg
D K
DK
Watkins
B A
BA
Stebler
W A
WA
Cramp
M B
MB
Yatvin
D000596:Amino Acids / Q000493:pharmacokinetics*; D001693:Biological Transport, Active / Q000187:drug effects; D004110:Diltiazem / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D006358:Hot Temperature; D007930:Leucine / Q000493:pharmacokinetics; D008715:Methionine / Q000493:pharmacokinetics; D011343:Procaine / Q000494:pharmacology; D011802:Quinidine / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
D000596:Amino Acids; D011343:Procaine; D008715:Methionine; D014700:Verapamil; D004110:Diltiazem; D007930:Leucine; D011802:Quinidine