Limb muscle regeneration in peripheral nerve autografts.
In a previous study we demonstrated regenerative growth of extraocular muscle within transplanted peripheral nerve autografts. The present study addresses the feasibility of inducing regeneration of limb muscle within autologous peripheral nerve implants in the gluteus medius of beagles. In six anesthetized animals, a 2-cm segment of the left infraorbital sensory nerve was removed from the nose and implanted between the cut ends of several muscle fascicles in the left gluteus medius. After 4 weeks, the nerve grafts were removed and examined by light and electron microscopy. Muscle fibers were seen surrounded by the epineurium of the implanted nerve along its entire length, growing in parallel with the long axis of the nerve. The regenerating fibers were closely associated with the basal lamina of degenerating myelinated and unmyelinated axons. This study suggests that limb muscle, like extraocular muscle, is capable of organized regenerative growth within peripheral nerve autografts.
Department of Ophthalmology, University of Kentucky, Lexington.
S P
SP
Christiansen
M
M
Madhat
R S
RS
Baker
D000818:Animals; D004285:Dogs; D005260:Female; D006614:Hindlimb; D008854:Microscopy, Electron; D009132:Muscles / Q000502:physiology* / Q000648:ultrastructure; D010525:Peripheral Nerves / Q000637:transplantation*; D012038:Regeneration; D013536:Suture Techniques; D014182:Transplantation, Autologous