Comparison of an enzyme-immunoassay with a radio-immunoassay method for the detection of the hepatitis markers anti-HBs, anti-HBc and HBsAg.
A comparative study was carried out on a radio-immunoassay (RIA) and enzyme-immunoassay (EIA) method for detecting the hepatitis markers anti-HBs, anti-HBc and HBsAg. The results indicated that the RIA and EIA were comparable for the HBsAg marker but that the RIA test was more sensitive for anti-HBs and more specific for anti-HBc. The conclusion was that if the EIA test is used for these markers, the laboratory and clinician must be aware of these limitations.
Department of Virology, University of the Witwatersrand, Johannesburg.
N K
NK
Blackburn
B D
BD
Schoub
K F
KF
O'Connell
D005069:Evaluation Studies as Topic; D006510:Hepatitis B Antibodies / Q000032:analysis*; D006512:Hepatitis B Core Antigens / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000032:analysis* / Q000276:immunology; D007124:Immunoenzyme Techniques; D011863:Radioimmunoassay
D006510:Hepatitis B Antibodies; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens