Serological evaluation of Escherichia coli-expressed human T-cell leukemia virus type I env, gag p24, and tax proteins.
Three proteins (env, gag, and tax) encoded by the human T-cell leukemia virus type I (HTLV-I) genome were cloned and expressed in Escherichia coli. The env protein contained a substantial part of the gp46 domain and a majority of the p21e domain. The gag protein contained all of p24 and portions of p19 and p15. In addition to these two structural proteins, a full-length tax (p40X) construct was obtained. All three recombinant proteins were purified to near homogeneity. When used in an immunoblot assay, the three recombinant proteins detected antibodies in more HTLV-I antibody-positive patient sera than did the corresponding native proteins. Antibodies to at least two of these three different gene products were detected in 98.4% of adult T-cell leukemia patients, 100% of HTLV-I-associated myelopathy patients, 97.4% of asymptomatic carriers, and 94% of uncharacterized HTLV-I-positive patients. Antibody to recombinant tax was found in 4.9% of adult T-cell leukemia patients, whereas antibody to recombinant env could not be detected. These recombinant proteins from three different gene products may be useful in detecting or confirming the presence of antibodies to HTLV-I.
Coates
S R
SR
Triton Biosciences Inc., Alameda, California 94501.
Harris
A J
AJ
Parkes
D L
DL
Smith
C M
CM
Liu
H L
HL
Akita
R W
RW
Ferrer
M M
MM
Sampson
E K
EK
Brandis
J W
JW
Sliwkowski
M X
MX
D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics*; D005069:Evaluation Studies as Topic; D015870:Gene Expression; D015686:Gene Products, env / Q000235:genetics* / Q000276:immunology; D015683:Gene Products, gag / Q000235:genetics* / Q000276:immunology; D015490:HTLV-I Infections / Q000276:immunology*; D006579:Heterozygote; D015368:Human T-lymphotropic virus 1 / Q000235:genetics* / Q000276:immunology; D006801:Humans; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012680:Sensitivity and Specificity; D015534:Trans-Activators / Q000235:genetics* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D015686:Gene Products, env; D015683:Gene Products, gag; D011994:Recombinant Proteins; D015534:Trans-Activators