The pancornulins: a group of basic low molecular weight proteins in mammalian epidermis and epithelium that may function as cornified envelope precursors.
1. A monoclonal antibody (HCE-2) to human epidermal and epithelial cornified envelopes identified a group of soluble basic protein precursors. 2. Using HCE-2, envelope-like staining was observed in the epidermis and stratified squamous epithelium of a number of mammalian species. 3. Basic polypeptides reactive to HCE-2 varied in size and number among the different animals. 4. In those species studied, HCE-2-reactive peptides were substrates for transglutaminase and protease treatment of cornified envelopes released HCE-2-reactive degradation products. 5. These results suggest a new family of proteins in mammalian epidermis that may function as cornified envelope precursors.
Department of Dermatology, Harvard Medical School, Massachusetts General Hospital, Boston 02114.
S B
SB
Phillips
J
J
Kubilus
A M
AM
Grassi
M L
ML
Goldaber
H P
HP
Baden
D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D004817:Epidermis / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D008322:Mammals; D008970:Molecular Weight; D011498:Protein Precursors / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D011506:Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D011498:Protein Precursors; D011506:Proteins