Comparison of some enrichment broths and growth media for the isolation of thermophilic campylobacters from surface water samples.
Three different enrichment broths and two selective growth media were compared for isolating thermophilic campylobacters by combined membrane filtration and enrichment techniques from surface waters of different physical, chemical and bacteriological characteristics. Fifty-two strains of campylobacters were isolated from total of 1668 cultures. The various broth/medium combinations did not affect the dominance of C. jejuni over C. coli (total 49 C. jejuni and three C. coli). The most efficient combinations of enrichment broth and growth media were either Oosterom broth/blood-free charcoal-cefoperazone-deoxycholate agar (CCDA) medium or blood-free charcoal-cefoperazone-deoxycholate (CCD) broth/CCDA medium. Modified Preston broth (sheep blood instead of horse blood) with either of the growth media gave significantly lower yields although it suppressed efficiently the growth of contaminants. Skirrow medium had lower selectivity than CCDA medium and gave slightly lower isolation rate. Enrichment time (24 or 48 h) did not affect the isolation frequency of campylobacters but longer enrichment time increased the growth of contaminants. Prefiltration through membranes of pore sizes 5.0 and 1.2 microns decreased the growth of contaminants. However, these membranes retain campylobacters and must be cultured to avoid underestimation. From more polluted waters campylobacters were isolated most frequently with CCD broth and CCDA medium.
National Public Health Institute, Department of Environmental Hygiene and Toxicology, Kuopio, Finland.
L K
LK
Korhonen
P J
PJ
Martikainen
D015373:Bacterial Typing Techniques; D002167:Campylobacter / Q000145:classification / Q000302:isolation & purification*; D016009:Chi-Square Distribution; D003470:Culture Media; D005618:Fresh Water; D006358:Hot Temperature; D012703:Serotyping; D014871:Water Microbiology
D003470:Culture Media