Visualization of apo B, fibrinogen/fibrin, and fibronectin in the intima of normal human aorta and large arteries and during atherosclerosis.
Apolipoprotein B (apo B), fibrinogen/fibrin, blood platelets, factor VIII-related antigen of the blood coagulation system, and smooth muscle cells (SMC) were identified in the intima of normal and atherosclerotic human aorta and large arteries by the indirect immunofluorescence technique. Fibrinogen/fibrin was revealed by a monoclonal antibody (monAb) against the C-terminal region of human fibrinogen A alpha-chain. Fibronectin was visualized by monAb to the cellular form and against an epitope shared by different fibronectin subunit variants. In normal intima, fatty streaks, small amounts of fibrinogen/fibrin together with large amounts of apo B were observed. Fibronectin detected by two types of monAb was not found in extracellular matrix (ECM), whereas cellular fibronectin encircled SMC. According to the data obtained, fibrinogen/fibrin accumulates in plaques as a result of intramural thrombus incorporation, blood insudation, intramural haemorrhage, and in or around cells, apparently macrophages.
Shekhonin
B V
BV
Institute of Experimental Cardiology, U.S.S.R. Cardiology Research Center, Moscow.
Tararak
E M
EM
Samokhin
G P
GP
Mitkevich
O V
OV
Mazurov
A V
AV
Vinogradov
D V
DV
Vlasik
T N
TN
Kalantarov
G F
GF
Koteliansky
V E
VE
D000368:Aged; D000911:Antibodies, Monoclonal; D001011:Aorta / Q000378:metabolism; D001055:Apolipoproteins B / Q000378:metabolism*; D001158:Arteries / Q000378:metabolism*; D001161:Arteriosclerosis / Q000378:metabolism*; D001792:Blood Platelets / Q000378:metabolism; D005260:Female; D005337:Fibrin / Q000378:metabolism*; D005340:Fibrinogen / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008875:Middle Aged; D014841:von Willebrand Factor / Q000378:metabolism
D000911:Antibodies, Monoclonal; D001055:Apolipoproteins B; D005353:Fibronectins; D014841:von Willebrand Factor; D005337:Fibrin; D005340:Fibrinogen