Application of automated image analysis to demonstrate the correlation between ras p21 expression and severity of gliomas.
We found a direct correlation between increasing ras p21 protein immunopositivity and severity of human glioma using computer-assisted, digital-image processing to quantify the amount of p21 immunoreactive to the monoclonal antibody RAP-5. We determined that there was a significant difference in reactivity between glioblastoma multiformes and more-differentiated astrocytomas (experiment-wise error less than 0.05). This result confirmed the conclusions made on the same tumors using standard light microscopy and visual examination. Immunohistochemistry quantized by automated image analysis may be a useful adjunct to current histopathological strategies since it decreases assay subjectivity and variation.
Department of Pathology, College of Medicine, Ohio State University, Columbus 43210.
M
M
Salgaller
L
L
Agius
A
A
Yates
D
D
Pearl
W
W
Roberts
R
R
Stephens
D000911:Antibodies, Monoclonal; D001322:Autoanalysis; D015415:Biomarkers / Q000032:analysis*; D001932:Brain Neoplasms / Q000473:pathology; D005910:Glioma / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D015689:Oncogene Protein p21(ras) / Q000032:analysis* / Q000096:biosynthesis
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D015689:Oncogene Protein p21(ras)