A slide enzyme-linked immunosorbent assay (SELISA) for the diagnosis of Babesia bovis infections and for the screening of Babesia-specific monoclonal antibodies.
A slide enzyme-linked immunosorbent assay (SELISA), a modification of the standard ELISA technique, was developed for detection of Babesia bovis antibodies in bovine sera. Smears of B. bovis-infected blood were used as the source of antigen in the test which was read using a light microscope. Monoclonal antibodies to defined B. bovis antigens were used to demonstrate the cellular specificity of the test. The SELISA was shown to be as sensitive as existing non-enzyme based serological tests for B. bovis. Comparative to the conventional ELISA technique, it was more economical and technically simpler, thus making it an ideal test for field application.
Department of Parasitology, University of Queensland, St. Lucia, Australia.
M W
MW
Kung'u
B V
BV
Goodger
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000913:Antibodies, Protozoan / Q000032:analysis*; D001403:Babesia / Q000276:immunology*; D001404:Babesiosis / Q000175:diagnosis*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006386:Hemagglutination Tests
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan