Lytic effect of Vibrio cholerae elastase on gram-positive and -negative bacteria.
Elastase of Vibrio cholerae caused the lysis of freshly grown cells of Gram-negative (Pseudomonas aeruginosa, Proteus vulgaris, Salmonella paratyphi A and Klebsiella pneumoniae) bacteria. Gram-positive (Staphylococcus aureus and S. epidermidis) organisms were resistant to this enzyme. Heat killed and lyophilized Gram-positive and -negative bacteria (except S. aureus and S. epidermidis) showed higher sensitivity to elastase. Both Gram-negative and -positive bacteria were lyzed maximally by elastase at pH 8.0. At this pH, lytic activity of elastase was maximum in Tris-HCl and glycine-NaOH buffers followed by Tris-maleate and cacodylate buffers.
Department of Microbiology, Panjab University, Chandigarh, India.
M
M
Kaur
M
M
Gupta
K K
KK
Tripathi
K G
KG
Gupta
D001433:Bacteriolysis; D006090:Gram-Negative Bacteria / Q000378:metabolism*; D006094:Gram-Positive Bacteria / Q000378:metabolism*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D010196:Pancreatic Elastase / Q000378:metabolism*; D014734:Vibrio cholerae / Q000201:enzymology*
D010196:Pancreatic Elastase