Isolated perfused cirrhotic human liver obtained from liver transplant patients: a feasibility study.
Cirrhotic livers obtained from eight patients who underwent orthotopic liver transplantation were perfused through the portal vein and hepatic artery in a closed recycling system for periods ranging from 2 to 7 hr. An average perfusion flow of 451 ml/min was used, with about 80% coming from the portal vein and 20% from the hepatic artery. The livers appeared to remain viable as assessed by gross appearance, stable portal vein and hepatic artery pressures, oxygen consumption and bile production. The extraction ratio of indocyanine green by the perfused livers averaged 0.098 (range = 0.023 to 0.168); that of propranolol averaged 0.445 (range = 0.126 to 0.813). Using the multiple-indicator dilution-curve method, shunts greater than 15 microns in diameter were demonstrated between the portal and hepatic veins in six of eight cases, whereas shunts from the hepatic artery to the hepatic veins were absent. Perfusion of human livers obtained during hepatic transplantation is a fairly simple procedure that will allow researchers to gain new insights into cirrhosis in humans.
Department of Medicine, André-Viallet Clinical Research Center, Montréal, Quebec, Canada.
J P
JP
Villeneuve
P M
PM
Huet
L
L
Gariepy
D
D
Fenyves
B
B
Willems
J
J
Côté
R
R
Lapointe
D
D
Marleau
D000792:Angiography; D005240:Feasibility Studies; D006801:Humans; D066298:In Vitro Techniques; D007201:Indicator Dilution Techniques; D007208:Indocyanine Green; D008099:Liver / Q000000981:diagnostic imaging / Q000378:metabolism; D008102:Liver Circulation; D008103:Liver Cirrhosis / Q000378:metabolism; D016031:Liver Transplantation; D008833:Microcirculation; D010477:Perfusion / Q000295:instrumentation; D011172:Portography; D011433:Propranolol / Q000493:pharmacokinetics; D009927:Tissue and Organ Procurement
D011433:Propranolol; D007208:Indocyanine Green