The DNA adenine methyltransferase (dam+) gene of bacteriophage T4 reverses the mutator phenotype of an Escherichia coli dam mutant.
The mutator phenotype of Escherichia coli dam mutants was found to be reversed by introduction of the bacteriophage T4 gene for DNA adenine methyltransferase. This precludes a direct role for the E. coli DNA adenine methyltransferase in mismatch repair, in addition to its role in strand discrimination, as suggested by earlier studies (S. L. Schlagman, S. Hattman, and M. G. Marinus, J. Bacteriol. 165:896-900, 1986).
Laboratory for Molecular Biology, CSIRO Division of Biotechnology, North Ryde, NSW, Australia.
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D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins; D005814:Genes, Viral; D008780:Methyltransferases / Q000235:genetics*; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D013604:T-Phages / Q000201:enzymology / Q000235:genetics*; D014764:Viral Proteins; D015678:Viral Structural Proteins / Q000235:genetics*
D029968:Escherichia coli Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; D008780:Methyltransferases; C058693:Dam methyltransferase; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); C499297:dam protein, Bacteriophage T4; C499294:dam protein, E coli