HLA-DR expression on human peritoneal macrophages in vivo and in vitro.
Qualitative, semi-quantitative (immuno-electronmicroscopy), and quantitative (radioimmunoassay) measurements were made of the in vivo and in vitro expression of HLA-DR on continuous ambulatory peritoneal dialysis (CAPD) patients' peritoneal macrophages (M phi) and on healthy persons' blood monocytes (MO). In vivo, great variation is seen in both the qualitative and (semi-) quantitative expression of HLA-DR in peritoneal M phi. After culturing for 5 to 20 h, CAPD patients' M phi with low to intermediate numbers of HLA-DR molecules per cell (25-80 x 10(3] showed a two- to threefold enhancement of HLA-DR expression. This enhancement was determined for the total peritoneal cell (PC) population and for the adherent subpopulation of peritoneal M phi and blood MO. CAPD patients whose cells initially had high numbers of HLA-DR molecules (80-110 x 10(3] showed no or only slight enhancement of HLA-DR expression when cultured.
Department of Cell Biology, Medical Faculty, Amsterdam, The Netherlands.
H J
HJ
Bos
G D
GD
Keizer
A J
AJ
vd Muijsenberg
R H
RH
Beelen
D000818:Animals; D000906:Antibodies; D006684:HLA-DR Antigens / Q000096:biosynthesis*; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D006801:Humans; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D008264:Macrophages / Q000276:immunology*; D008297:Male; D008854:Microscopy, Electron; D010529:Peritoneal Cavity / Q000166:cytology; D010531:Peritoneal Dialysis, Continuous Ambulatory; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D000906:Antibodies; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II