Crystallization and preliminary x-ray investigation of colicin E3 in complex with its immunity protein.
Crystals of the colicin E3-immunity protein complex have been grown from solutions of citrate at pH 5.6. The crystals are monoclinic, space group P2(1), with unit cell dimensions a = 67.71, b = 196.67, c = 85.58 A, and beta = 113.67 degrees. The crystals diffract to 3-A resolution and are stable in the x-ray beam for at least a day. Although the stoichiometry of the complex in solution is 1:1 there are two, three, or four such binary complex molecules in the asymmetric unit.
Frolow
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Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.
Shoham
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D001426:Bacterial Proteins / Q000302:isolation & purification; D003087:Colicins / Q000037:antagonists & inhibitors*; D003460:Crystallization; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D011487:Protein Conformation; D014961:X-Ray Diffraction
D001426:Bacterial Proteins; D003087:Colicins; D029968:Escherichia coli Proteins; C488855:ceiC protein, E coli; C007104:colicin immunity proteins