Structural studies on native and chemically modified storage proteins from rapeseed (Brassica napus L.) and related plant proteins.
Recent data on the structure and chemical modification of the two main storage proteins of rapeseed, the high-molecular mass 12 S globulin and the low-molecular mass 2 S protein (napin) are summarized and compared with those of related seed proteins. The 12 S globulin is built up of six subunits forming a quaternary structure which can be approximated by the model of a trigonal antiprism. The subunits, composed of a larger and a smaller polypeptide chain each, have a two-domain structure which is typical for all related plant proteins. These are characterized by a sedimentation coefficient of 11-13 S, a molecular mass of 300,000-360,000 g/mol and a high percentage of beta-sheet conformation. Increasing succinylation results in a step-by-step dissociation up to the subunits and to an unfolding of the latter at a critical level of modification amounting to 60-70%. These structural changes affect the functional properties remarkably. The napin fraction comprises a group of closely related and highly basic proteins with molecular masses of 12,000-14,000 g/mol, a high content of sulphur-containing amino acids and rich in helical conformation. They are built up of a larger and a smaller disulphide bridged polypeptide chain. Acylation does not abolish the secondary or tertiary structure which are stabilized by inter- and intrachain disulphide bonds. Acylation results, however, in a stabilization of the protein against heat-induced aggregation.
Central Institute of Nutrition in Potsdam-Rehbrücke, Academy of Sciences of the GDR.
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Schwenke
D055516:2S Albumins, Plant; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001937:Brassica / Q000032:analysis*; D005916:Globulins / Q000032:analysis; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000032:analysis*; D011487:Protein Conformation
D055516:2S Albumins, Plant; D000596:Amino Acids; D005916:Globulins; D046911:Macromolecular Substances; D010940:Plant Proteins; C050363:napin protein, Brassica napus