Evidence for an additional archaebacterial gene cluster in Halobacterium marismortui encoding ribosomal proteins HL46e and HL30.
A small and extremely basic ribosomal protein (HL46e) has been purified from Halobacterium marismortui using reversed-phase high-performance liquid chromatography (HPLC). The amino acid sequence of the protein was determined by automated N-terminal and internal sequence analysis. Comparison of this sequence with other ribosomal protein sequences from eubacteria, archaebacteria and eukaryotes revealed a strong homology to SL46e from Sulfolobus solfataricus, YeaL46 from yeast and RL39 from rat. No significant sequence similarly was found to any eubacterial ribosomal protein so far known. Using a specific oligonucleotide probe the HL46e gene was identified, cloned and the nucleotide sequence including the 5'- and 3'-flanking regions were analysed. The HL46e gene is followed by the gene coding for HL30. A putative halobacterial promoter sequence with the motive 'TTTAAA' has been localized 32 bp upstream of the HL46e gene and a putative terminator sequence localized downstream from the HL30 gene. An equivalent to this HL46e/HL30 operon is apparently not present in Escherichia coli.
Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann, Berlin, Germany.
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Bergmann
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Arndt
D000595:Amino Acid Sequence; D019843:Archaeal Proteins; D001483:Base Sequence; D005798:Genes, Bacterial; D006217:Halobacterium / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D015345:Oligonucleotide Probes; D015183:Restriction Mapping; D012269:Ribosomal Proteins / Q000235:genetics* / Q000302:isolation & purification; D012270:Ribosomes / Q000378:metabolism / Q000648:ultrastructure; D012689:Sequence Homology, Nucleic Acid
D019843:Archaeal Proteins; C065230:HL30 protein, Halobacterium marismortui; D015345:Oligonucleotide Probes; D012269:Ribosomal Proteins; C065982:ribosomal protein HL46e, Haloarcula marismortui