A noninstrumented quantitative test system and its application for determining cholesterol concentration in whole blood.
A novel noninstrumented technology has been developed for quantifying analytes of clinical interest in biological fluids. Application of this technology is exemplified by the development of a quantitative cholesterol test with performance equivalent to state-of-the-art instrumented methods. The assay chemistry combines two separate processes located in different areas of a test strip: enzymatic action on serum cholesterol to produce hydrogen peroxide (5 x 10 mm enzyme reagent pad) and quantification of the hydrogen peroxide (5 x 70 mm measurement region). Color bands are formed in the measurement area through the use of a redox-coupled indicator system. The height of the color band on the strip is directly proportional to the sample cholesterol concentration. A one-step cassette contains all components necessary to run the test and includes blood filtration and automatic sample measurement, so that unmeasured finger-stick whole-blood specimens can be analyzed by the non-technically trained user. The test is complete in less than 15 min, is read visually like a thermometer, and gives results that are in excellent correlation with established instrumented methods.
ChemTrak, Inc., Sunnyvale, CA 94086.
M P
MP
Allen
A
A
DeLizza
U
U
Ramel
H
H
Jeong
P
P
Singh
D001800:Blood Specimen Collection; D002784:Cholesterol / Q000097:blood*; D002854:Chromatography, Paper; D006801:Humans; D011934:Reagent Strips; D013696:Temperature
D011934:Reagent Strips; D002784:Cholesterol