Sperm bound to zona pellucida in hemizona assay demonstrate acrosome reaction when stained with T-6 antibody.
A monoclonal antibody, T-6, useful for detecting acrosome-reacted sperm based on an immunofluorescent assay, was employed to evaluate acrosomal status of human sperm that were tightly bound to hemisected human zonae pellucidae (hemizona assay). Over 90% of the bound sperm evaluated exhibited immunofluorescent patterns indicative of acrosome reaction. This staining method for evaluating the acrosomal status of sperm bound to the zona pellucida may enable definition of a group of male infertility patients heretofore not recognized.
Jones Institute for Reproductive Medicine, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk 23510.
C C
CC
Coddington
D L
DL
Fulgham
N J
NJ
Alexander
D
D
Johnson
J C
JC
Herr
G D
GD
Hodgen
D000177:Acrosome / Q000502:physiology* / Q000648:ultrastructure; D000911:Antibodies, Monoclonal; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis; D008297:Male; D008722:Methods; D010063:Ovum / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D013094:Spermatozoa / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D015044:Zona Pellucida / Q000378:metabolism*
D000911:Antibodies, Monoclonal