Preliminary crystallographic analysis of the plant pathogenic factor, pectate lyase C from Erwinia chrysanthemi.
Pectate lyases are saccharide-binding enzymes that degrade plant cell walls. One pectate lyase from Erwinia chrysanthemi (EC16), termed pectate lyase C, has been crystallized from ammonium sulfate. The preliminary x-ray diffraction analysis indicates that the crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit cell dimensions, a = 73.4 A, b = 80.3 A, and c = 95.1 A. The crystals diffract to a resolution of 2.2 A and have one molecule/asymmetric unit.
Department of Biochemistry, University of California, Riverside 92521.
M D
MD
Yoder
D A
DA
DeChaine
F
F
Jurnak
D000645:Ammonium Sulfate; D003001:Cloning, Molecular; D003460:Crystallization; D004885:Erwinia / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D004926:Escherichia coli / Q000235:genetics; D010944:Plants / Q000382:microbiology; D010957:Plasmids; D011133:Polysaccharide-Lyases / Q000235:genetics / Q000302:isolation & purification; D014961:X-Ray Diffraction
D011133:Polysaccharide-Lyases; C023911:pectate lyase; D000645:Ammonium Sulfate