NMR studies of interactions of ligands with dihydrofolate reductase.
NMR spectroscopy is a useful technique for studying interactions, conformations and dynamic processes within ligand-protein complexes. Several examples of the application of the method to studies of complexes of anti-folate drugs with their target enzyme, dihydrofolate reductase, are discussed.
National Institute for Medical Research, Mill Hill, London, U.K.
J
J
Feeney
D004347:Drug Interactions; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D008727:Methotrexate / Q000378:metabolism*; D008968:Molecular Conformation; D009154:Mutation; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D014295:Trimethoprim / Q000378:metabolism*
D008024:Ligands; D014295:Trimethoprim; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate