A simple procedure for isolation of tumor-associated antigens by affinity chromatography using fucose-specific Aleuria aurantia lectin.
An affinity column containing L-fucose specific Aleuria aurantia lectin was used for the efficient separation of tumor-associated antigens. Five of six glycoconjugates antigens tested, carcinoembryonic antigen (CEA), CA19-9, sialyl Lewis X-i, DU-PAN-2 and CA125, bound to the affinity gel and eluted in high yield with 20 mM L-fucose, but alpha-fetoprotein, which is known to contain fucose in the carbohydrate chain, passed through the column. This column was also proved to be useful for group separation of CA19-9, sialyl Lewis X-i and Leb antigens from a serum sample with gastric cancer.
Department of Legal Medicine, School of Medicine, Gunma University, Maebashi, Japan.
S
S
Yazawa
N
N
Kochibe
T
T
Asao
D000911:Antibodies, Monoclonal; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000097:blood / Q000302:isolation & purification*; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity / Q000379:methods*; D005643:Fucose; D006801:Humans; D007124:Immunoenzyme Techniques; D037102:Lectins; D008969:Molecular Sequence Data
D000911:Antibodies, Monoclonal; D015295:Antigens, Tumor-Associated, Carbohydrate; D037102:Lectins; C064549:lectin, Aleuria aurantia; D005643:Fucose