High sensitivity of the LEC rat liver to the carcinogenic effect of diethylnitrosamine.
The initiation sensitivity of the liver of the LEC (Long-Evans with a cinnamon-like coat color) rat, a new mutant strain with a high incidence of spontaneous liver tumors, was studied by treatment with low doses of diethylnitrosamine (DEN) coupled with modified Solt and Farber's selection. LEC and control LEA (Long-Evans with an agouti coat color) rats received i.p. injections of 10 mg/kg of DEN, then selected by feeding with a diet containing 0.02% 2-acetylaminofluorene (AAF) for 7 days combined with partial hepatectomy (PH). The numbers of placental glutathione S-transferase (GST-P)-positive foci in the livers of LEC rats were 10 times higher than those in LEA rats. These results suggested a high sensitivity of the LEC rat liver to the carcinogenic effect of DEN. The association between initiation sensitivity and spontaneous liver-tumor development and the possible usefulness of the LEC rat for in vivo short-term tests of hepatocarcinogens are discussed.
Department of Pathology, Sapporo Medical College, Japan.
H
H
Takahashi
K
K
Enomoto
Y
Y
Nakajima
M
M
Mori
D015073:2-Acetylaminofluorene / Q000008:administration & dosage / Q000633:toxicity; D000818:Animals; D004052:Diethylnitrosamine / Q000633:toxicity*; D004785:Environmental Pollutants / Q000633:toxicity; D005260:Female; D005982:Glutathione Transferase / Q000032:analysis*; D006498:Hepatectomy; D006505:Hepatitis / Q000201:enzymology / Q000235:genetics; D007124:Immunoenzyme Techniques; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced* / Q000201:enzymology; D008297:Male; D008954:Models, Biological; D051381:Rats; D011922:Rats, Mutant Strains / Q000235:genetics; D013997:Time Factors
D004785:Environmental Pollutants; D004052:Diethylnitrosamine; D015073:2-Acetylaminofluorene; D005982:Glutathione Transferase