Tumour-related expression of a translation-elongation factor-like protein.
We have identified a tumour-related 43 kd cytoplasmic protein (LC/p43) using a monoclonal antibody against the total proteins of human hepatoma cell line PLC/PRF/5. LC/p43 is preferentially expressed in a variety of tumours of human and animal origin, whereas no expression was detected in several normal adult tissues tested. LC/p43 expression was induced in rodent fibroblasts upon transfection with several viral oncogenes. Expression in non-transformed peripheral blood lymphocytes could be induced by treatment with phytohaemagglutinin (PHA) and subsequent culture with interleukin II, whereas retinoic acid treatment of transformed cells caused a drastic reduction of the antigen in the cells. Sequence analysis of three tryptic peptides of LC/p43 revealed 50-70% homology to different domains of the eukaryotic and prokaryotic translation-elongation factors EF1-alpha and EF-Tu, respectively.
Max-Planck-Institut für Biochemie, Department of Virus Research, Martinsried bei München, Federal Republic of West Germany.
I
I
Koch
P H
PH
Hofschneider
F
F
Lottspeich
C
C
Eckerskorn
R
R
Koshy
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000473:pathology; D005347:Fibroblasts / Q000276:immunology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D015151:Immunoblotting; D007376:Interleukin-2 / Q000494:pharmacology; D008113:Liver Neoplasms / Q000276:immunology / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000276:immunology / Q000473:pathology; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010445:Peptide Elongation Factors / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D010641:Phenotype; D010835:Phytohemagglutinins / Q000494:pharmacology; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection; D014212:Tretinoin / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D007376:Interleukin-2; D010445:Peptide Elongation Factors; D010835:Phytohemagglutinins; D014212:Tretinoin