Tissue distribution of avidin and streptavidin injected to mice. Effect of avidin carbohydrate, streptavidin truncation and exogenous biotin.
Radioionated avidin and streptavidin were characterized for their biodistribution and tissue association in Balb/c mice, in comparison to their interaction with cells in vitro. Binding of avidin to spleen and bone-marrow cells in vitro was up to 20-fold higher than that of streptavidin, but when tested in vivo avidin clearance from blood and tissues was considerably faster than that of streptavidin. Levels of avidin at 24 h after an intravenous injection were below 1% (of the injected dose/mass tissue) in most organs. Non-glycosylated avidin was similar in its biodistribution to native avidin. Native streptavidin exhibited higher and prolonged tissue association with 5-10% levels in lung, liver, spleen, kidney and blood, whereas its truncated form showed low tissue levels (1-3%) but a remarkably high affinity to the kidney (80%). Exogenous biotin did not affect streptavidin distribution in vivo but caused a 2-7-fold increase in the retention of avidin (but not non-glycodylated avidin) in some of the organs.
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
B
B
Schechter
R
R
Silberman
R
R
Arnon
M
M
Wilchek
D000818:Animals; D001360:Avidin / Q000378:metabolism / Q000493:pharmacokinetics*; D001426:Bacterial Proteins / Q000378:metabolism / Q000493:pharmacokinetics*; D001710:Biotin / Q000494:pharmacology*; D001853:Bone Marrow / Q000378:metabolism; D006031:Glycosylation; D007457:Iodine Radioisotopes; D008297:Male; D008657:Metabolic Clearance Rate; D051379:Mice; D008807:Mice, Inbred BALB C; D011485:Protein Binding; D011865:Radioisotope Dilution Technique; D013154:Spleen / Q000378:metabolism; D019809:Streptavidin; D014018:Tissue Distribution
D001426:Bacterial Proteins; D007457:Iodine Radioisotopes; D001360:Avidin; D001710:Biotin; D019809:Streptavidin