[Use of a new silicon chamber system for carrying out immunologic technics with blood and bone marrow samples and histologic specimens].
We are reporting for the first time on the successful application of the newly developed method of Silicon-Chamber-System (SCS). For this purpose we are utilizing a commonly manufactured silicon sealant by which we are able to obtain reaction fields of any size and number. We are applying the sealant on cell- and on tissue-slides without any special preparation. These reaction fields on the slides allow us to immunohistochemically analyse cells with multiple different monoclonal antibodies without running great risk of a cross-reaction between the different immunoreagents. It is a simple inexpensive and convenient method, giving us the opportunity to analyze elaborate cell material.
Abteilung für Pädiatrische Hämatologie und Onkologie, Universität Frankfurt.
U
U
Ebener
S
S
Hauser
S
S
Wehner
B
B
Kornhuber
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis; D001773:Blood Cells / Q000473:pathology*; D001853:Bone Marrow / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D012826:Silicone Elastomers; D013048:Specimen Handling / Q000295:instrumentation*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D014408:Biomarkers, Tumor; D012826:Silicone Elastomers
Anwendung eines neuen Silikon-Kammersystems (SCS) zur Durchführung immunologischer Detektionssysteme an Blut-/KM-Ausstrichen und an histologischen Präparaten.