Genotoxicity of benzo[a]pyrene, 2-nitrofluorene and airborne particulates in the DNA-repair host-mediated assay.
The genotoxic activity of benzo[a]pyrene (BAP), 2-nitrofluorene (NF) and airborne particulate matter was evaluated in the DNA-repair host-mediated assay after intraperitoneal or intratracheal administration. Dimethylnitrosamine (DMNA), used as a positive control, showed a genotoxic effect after both intraperitoneal and intratracheal administration, the strongest effect being found in liver, followed by lungs and kidneys, whereas a weak effect was observed in the spleen. In general no difference in genotoxicity was found between the 2 administration routes used. For BAP, although clearly positive in vitro, a moderate dose-dependent effect was found only in the liver after intraperitoneal administration. NF, which was positive in vitro both with and without a metabolizing system, produced no genotoxic effect in any of the organs tested after intraperitoneal administration. Extracts of airborne particulate matter which were genotoxic in vitro failed to cause a genotoxic effect in vivo by either route of administration. Possible explanations for the differences between the data obtained in vitro and in vivo are discussed.
Heussen
G A
GA
Department of Toxicology, Agricultural University, Wageningen, The Netherlands.
Post
J G
JG
Alink
G M
GM
D000284:Administration, Oral; D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D001140:Aroclors / Q000633:toxicity; D001564:Benzo(a)pyrene / Q000633:toxicity*; D004260:DNA Repair; D004269:DNA, Bacterial / Q000187:drug effects*; D004128:Dimethylnitrosamine / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000235:genetics; D005449:Fluorenes / Q000633:toxicity*; D007274:Injections, Intraperitoneal; D007668:Kidney / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D008168:Lung / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000187:drug effects
D000393:Air Pollutants; D001140:Aroclors; D004269:DNA, Bacterial; D005449:Fluorenes; D001564:Benzo(a)pyrene; D004128:Dimethylnitrosamine