Effect of glucose in mice after acute experimental poisoning with arsenic trioxide (As2O3).
Carbohydrate depletion (glucose and glycogen) was reported to be a major problem in acute arsenic poisoning. In the present paper the effectiveness of glucose substitution was investigated in mice after acute experimental poisoning with As2O3. Four groups of ten mice each received As2O3, 12.9 mg/kg, s.c. After the injection the first group remained without further treatment, the second received saline every 2 h, the third 5% glucose, and the fourth 5% glucose +0.12 IE insulin/kg i.p. Groups 5 and 6, five mice each, received either saline or glucose only. Group 7, five mice, remained without any treatment. Immediately after death the livers were removed for the enzymatic determination of glucose and glycogen. Mice receiving As2O3 only died within 22 h. The mean survival time was 12.4 h. In mice receiving As2O3 and after that saline, glucose, or glucose + insulin, an increase in the survival time to 30.8, 40.7, and 43.6 h, respectively, was observed. All mice which died showed a significant decrease in the liver glucose and glycogen content, compared to control animals. In livers of survivors, the glucose and glycogen content was not different to the control groups. The data support the assumption that carbohydrate depletion is an important factor in arsenic toxicity, and its substitution should be considered in the treatment of arsenic poisoning.
Reichl
F X
FX
Walther Straub-Institut für Pharmakologie and Toxikologie, Ludwig-Maximilians-Universität München, Federal Republic of Germany.
Szinicz
L
L
Kreppel
H
H
Fichtl
B
B
Forth
W
W
D000818:Animals; D020261:Arsenic Poisoning; D000077237:Arsenic Trioxide; D001152:Arsenicals; D003643:Death; D003967:Diarrhea / Q000139:chemically induced; D005947:Glucose / Q000494:pharmacology* / Q000627:therapeutic use; D007328:Insulin / Q000627:therapeutic use; D008297:Male; D051379:Mice; D010087:Oxides; D012965:Sodium Chloride / Q000494:pharmacology
D001152:Arsenicals; D007328:Insulin; D010087:Oxides; D012965:Sodium Chloride; D005947:Glucose; D000077237:Arsenic Trioxide