Accurately initiated, enhancer-dependent transcription by RNA polymerase I in yeast extracts.
Nuclear extracts from Saccharomyces cerevisiae support initiation by RNA polymerase I at the 35 S rRNA promoter. Initiation occurs at the same site and is stimulated by the polymerase I enhancer element to about the same extent in vitro as in vivo.
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Department of Cell Biology, Stanford University School of Medicine, California 94305-5400.
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D000546:Amanitins / Q000494:pharmacology; D002467:Cell Nucleus / Q000502:physiology*; D002474:Cell-Free System; D004252:DNA Mutational Analysis; D004742:Enhancer Elements, Genetic; D015966:Gene Expression Regulation, Fungal; D010957:Plasmids; D012318:RNA Polymerase I / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic
D000546:Amanitins; D012318:RNA Polymerase I