Immunohistochemical localization of parathyroid hormone-related protein in parathyroid adenoma and hyperplasia.
Parathyroid hormone-related protein (PTHrP) is invoked as the cause of humoral hypercalcaemia of malignancy (HHM); it is contained in the keratinocyte layer of normal skin; and there is evidence that is is produced by fetal parathyroids. Antibodies against synthetic PTHrP peptides have been raised in rabbits and sheep. This immunohistochemical study has found that primary parathyroid adenomata and hyperplastic glands from patients with chronic renal failure stain positively with antisera against PTHrP(1-34) and PTHrP(50-69). Primary hyperplastic glands are negative. No staining with anti-PTHrP(106-141) antiserum could be detected immunohistochemically in any of the parathyroid adenomata or hyperplasia.
St Vincent's Institute of Medical Research, St Vincent's Hospital, Melbourne, Australia.
J A
JA
Danks
P R
PR
Ebeling
J A
JA
Hayman
H
H
Diefenbach-Jagger
F M
FM
Collier
V
V
Grill
J
J
Southby
J M
JM
Moseley
S T
ST
Chou
T J
TJ
Martin
D000236:Adenoma / Q000032:analysis*; D015153:Blotting, Western; D006801:Humans; D006961:Hyperparathyroidism / Q000150:complications; D006965:Hyperplasia / Q000378:metabolism; D007124:Immunoenzyme Techniques; D007676:Kidney Failure, Chronic / Q000150:complications; D009363:Neoplasm Proteins / Q000032:analysis; D010280:Parathyroid Glands / Q000032:analysis / Q000473:pathology*; D044162:Parathyroid Hormone-Related Protein; D010282:Parathyroid Neoplasms / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis; D011506:Proteins / Q000032:analysis*
D009363:Neoplasm Proteins; C470121:PTHLH protein, human; D044162:Parathyroid Hormone-Related Protein; D010446:Peptide Fragments; D011506:Proteins; C054296:parathyroid hormone-related protein (1-34)