In vivo import of Candida tropicalis hydratase-dehydrogenase-epimerase into peroxisomes of Candida albicans.
We present a system for studying peroxisomal protein targeting in Candida. We have expressed the Candida tropicalis gene encoding hydratase-dehydrogenase-epimerase (HDE) in Candida albicans. Immunoblot analyses of C. albicans transformants demonstrate the presence of oleic-acid inducible HDE (100 kDa) in peroxisomes of transformed cells, but not of control cells. Peroxisomes isolated from transformed cells show increased beta-hydroxyacyl-CoA dehydrogenase specific activity, indicating that HDE is imported into peroxisomes of C. albicans where it is enzymatically active. C. albicans provides a useful model for the study of protein targeting to peroxisomes in vivo.
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada.
J D
JD
Aitchison
R A
RA
Rachubinski
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D015139:Blotting, Southern; D002175:Candida / Q000201:enzymology* / Q000235:genetics; D002176:Candida albicans / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D004746:Enoyl-CoA Hydratase / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D004790:Enzyme Induction; D005800:Genes, Fungal; D006836:Hydro-Lyases / Q000378:metabolism*; D007535:Isomerases / Q000378:metabolism*; D008830:Microbodies / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D063994:Peroxisomal Bifunctional Enzyme; D019998:Racemases and Epimerases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D014170:Transformation, Genetic
D009097:Multienzyme Complexes; D010088:Oxidoreductases; D015094:3-Hydroxyacyl CoA Dehydrogenases; D006836:Hydro-Lyases; D004746:Enoyl-CoA Hydratase; D063994:Peroxisomal Bifunctional Enzyme; D007535:Isomerases; D019998:Racemases and Epimerases