Complement mediated serum cytotoxicity against oligodendrocytes: a comparison with other cells of the oligodendrocyte-type 2 astrocyte lineage.
Rat oligodendrocytes are known to be susceptible to complement attack when exposed to homologous serum as a consequence of anti-myelin antibody independent classical pathway complement activation and attack. We have now compared this susceptibility with that of other cells of the oligodendrocyte-type 2 astrocyte (O-2A) lineage, and show that while type 2 astrocytes are not sensitive, O-2A progenitors are only relatively resistant to serum cytotoxicity, higher concentrations of complement resulting in cell damage. The implications of these findings for the pathogenesis of demyelinating disease are discussed.
Scolding
N J
NJ
Section of Neurology, University of Cambridge Clinical School, Addenbrooke's Hospital, U.K.
Morgan
B P
BP
Campbell
A K
AK
Compston
D A
DA
D000818:Animals; D001253:Astrocytes / Q000276:immunology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003165:Complement System Proteins / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic; D005455:Fluorescent Antibody Technique; D009186:Myelin Sheath / Q000276:immunology; D009457:Neuroglia / Q000187:drug effects; D009836:Oligodendroglia / Q000187:drug effects / Q000276:immunology*; D011107:Polylysine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D011107:Polylysine; D003165:Complement System Proteins