[The effect of Ha-ras oncogene on cells immortalized by the gene for polyomavirus large T-antigen].
Activated human Ha-ras oncogene cloned on the plasmid pEJras6,6 was transfected into REF (LT) cells immortalized by the gene for large T-antigen of the polyoma virus. The cells were shown to become completely transformed (in the terms of morphology and tumorogeneity) only after three cycles of transfection with the plasmid pEJras6,6. The integrated sequences of the plasmid pEJras6,6 and the ras oncogene product p21Ha-ras were detected in cells only after their selection in the nude mice (in the cell culture REF (LT) ras X 3tu obtained from the tumor and directly in the tumor cells). Thus, after sequential transfections with a c-Ha-ras oncogene we developed cell cultures on the different stages of transformation process.
E V
EV
Komissarova
D D
DD
Spitkovskiĭ
N N
NN
Mazurenko
A G
AG
Tatosian
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D011905:Genes, ras; D010957:Plasmids; D051381:Rats; D014162:Transfection
D000952:Antigens, Polyomavirus Transforming
Deĭstvie onkogena Ha-ras na kletki, immortalizovannye genom bol'shogo T-antigena virusa poliomy.