The influence of hydrogel functional groups on cell behavior.
This study provides information on the behavior of macrophages on the surface of the subcutaneously implanted hydrogel strips. Hydrogel containing -OH, -CO-NH-, and (CH3)2N- groups induced a spreading of macrophages on the implants. The materials containing -SO3H groups slightly, and materials containing -COOH groups more intensively, inhibited spreading of the macrophages. The fusion of macrophages into multinucleate cells was inhibited on the surface of materials containing acidic groups (-SO3H, -COOH) and increased on the hydrogels containing 30 mol% of alkaline (CH3)2N- groups in comparison with hydrogels containing -OH or -CO -NH- groups. The differences of the behavior of macrophages on the surface of individual types of hydrogels are probably independent on the adsorption of plasma fibronectin onto the hydrogels. The correlation between the macrophages spreading and fusion and surface charge of the hydrogel implant can be hypothetically explained by electrostatic interaction between macrophages cell membrane and implant.
Department of Anatomy, Charles University, Medical Faculty, Prague, Czechoslovakia.
K
K
Smetana
J
J
Vacík
D
D
Soucková
Z
Z
Krcová
J
J
Sulc
D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion; D002459:Cell Fusion; D002462:Cell Membrane / Q000502:physiology; D004563:Electrochemistry; D005260:Female; D005549:Foreign-Body Reaction / Q000473:pathology / Q000503:physiopathology; D015726:Giant Cells / Q000473:pathology*; D015743:Giant Cells, Foreign-Body / Q000473:pathology* / Q000502:physiology; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D008297:Male; D008422:Materials Testing; D011092:Polyethylene Glycols; D019736:Prostheses and Implants; D051381:Rats; D011919:Rats, Inbred Strains; D013499:Surface Properties
D001672:Biocompatible Materials; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols