Protective effect of sofalcone and 16,16-dimethyl-PGE2 on isolated rat gastric cells.
The effects of 16,16-dimethylprostaglandin E2 (dm-PGE2) and sofalcone on damage caused by ethanol in surface epithelial cells isolated from rat stomach were examined. The surface epithelial cells (SECs) accounted for 83% of the isolated gastric mucosal cells, mucus neck cells for 5%, parietal cells for 9%, and chief cells for 3%. To suspensions of SEC, either dm-PGE2 at concentrations of 10(-7), 10(-6), or 10(-5) M or sofalcone at concentrations from 5 X 10(-6) to 10(-4) M was added; some cells were treated with neither drug. Ten minutes later, ethanol was added to each cell suspension to a final concentration of 15%, and 5 min later the viability was evaluated by trypan blue exclusion. At 10(-6) M, dm-PGE2 reduced ethanol-induced cell damage most strongly (p less than 0.001). Sofalcone also helped to prevent cell damage caused by ethanol in a dose-dependent manner. These results suggest that dm-PGE2 and sofalcone protect gastric mucosa not only from gross visible damage but they directly protect gastric cells from damage.
Third Department of Internal Medicine, Osaka City University Medical School, Japan.
K
K
Kobayashi
T
T
Arakawa
A
A
Nakamura
H
H
Nakamura
D015064:16,16-Dimethylprostaglandin E2 / Q000494:pharmacology*; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D002599:Chalcone / Q000031:analogs & derivatives* / Q000494:pharmacology; D047188:Chalcones; D002927:Cimetidine / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D005753:Gastric Mucosa / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D008855:Microscopy, Electron, Scanning; D051381:Rats; D011919:Rats, Inbred Strains
D000897:Anti-Ulcer Agents; D047188:Chalcones; C035032:sofalcone; D000431:Ethanol; D002599:Chalcone; D002927:Cimetidine; D015064:16,16-Dimethylprostaglandin E2