Serum antibody response to Mycoplasma hyopneumoniae measured by enzyme-linked immunosorbent assay after experimental and natural infection of pigs.
Studies were conducted under experimental and field conditions to determine the effect of infection with M. hyopneumoniae on the immune response in serum as measured by ELISA. Following intratracheal challenge or contact exposure, serologically negative pigs derived from mycoplasma-free piggeries developed an immune response within 10 days. This response continued to rise for a further 50 days. In a field study in a commercial piggery, no animals (0/44) were observed to have M. hyopneumoniae antibodies at day 86 of life. However between day 86 and day 144, 97.7% (42/43) animals sero-converted. These results are discussed in terms of infection spread, particularly in the grower/finisher shed.
Sheldrake
R F
RF
NSW Agriculture and Fisheries, Elizabeth Macarthur Agricultural Institute, Menangle.
Gardner
I A
IA
Saunders
M M
MM
Romalis
L F
LF
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D009174:Mycoplasma / Q000276:immunology*; D045729:Pneumonia of Swine, Mycoplasmal / Q000276:immunology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology*
D000907:Antibodies, Bacterial