Preparation of fluorescence-labeled and cross-linked subtilisin.
Streptomyces subtilisin inhibitor (SSI) is a protein characterized by both its potent inhibitory activity toward subtilisin and its structure, composed of two homologous subunits. It binds two molecules of subtilisin to form a tetrameric complex. Intermolecularly cross-linked subtilisin is expected to form a polymeric complex with SSI. This could provide a useful model of protein-protein association. Therefore, preparation of fluorescence-labeled and cross-linked subtilisin was carried out.
Tanizawa
K
K
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapparo, Japan.
Mano
T
T
Kanaoka
Y
Y
D001426:Bacterial Proteins; D055598:Chemical Phenomena; D002621:Chemistry; D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D013381:Subtilisins / Q000032:analysis*
D001426:Bacterial Proteins; D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; C018150:subtilisin inhibitor protein, Streptomyces; D013381:Subtilisins