Two-dimensional crystallization of reaction centers from Chloroflexus aurantiacus.
Two-dimensional crystals of photosynthetic reaction centers from Chloroflexus aurantiacus were obtained from protein-lipid-detergent micelles by detergent dialysis. The size of crystals was up to 2 microns. Some of them were multilayered crystals. However, other crystal forms were also observed. Preliminary image processing analysis showed that crystals of one crystal form referred to two-sided plane group p2 and had the following unit cell parameters: a = 17.6 nm, b = 18.0 nm, gamma = 84 degrees. The contour map of the crystal stain-excluding region was calculated by the Fourier-filtering procedure at about 2 nm resolution.
Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow.
A N
AN
Barnakov
V V
VV
Demin
A P
AP
Kuzin
A A
AA
Zargarov
A S
AS
Zolotarev
N G
NG
Abdulaev
D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000648:ultrastructure; D003460:Crystallization; D008854:Microscopy, Electron; D045322:Photosynthetic Reaction Center Complex Proteins
D001426:Bacterial Proteins; D045322:Photosynthetic Reaction Center Complex Proteins