Characterization of cell-surface receptors for monoclonal-nonspecific suppressor factor (MNSF).
Monoclonal-nonspecific suppressor factor (MNSF) is a lymphokine derived from murine T cell hybridoma. The target tissues are both LPS-stimulated B cells and Con A-stimulated T cells. Since the action of MNSF may be mediated by its binding to specific cell surface receptors, we characterized the mode of this binding. The purified MNSF was labeled with 125I, using the Bolton-Hunter reagent. The labeled MNSF bound specifically to a single class of receptor (300 receptors per cell) on mitogen-stimulated murine B cells or T cells with an affinity of 16 pM at 24 degrees C, in the presence of sodium azide. Competitive experiments showed that MNSF bound to the specific receptor and that the binding was not shared with IL2, IFN-gamma, and TNF. Various cell types were surveyed for the capacity to specifically bind 125I-MNSF. 125I-MNSF bound to MOPC-31C (a murine plasmacytoma line) and to EL4 (a murine T lymphoma line). The presence of specific binding correlates with the capacity of the cells to respond to MNSF. These data support the view that like other polypeptide hormones, the action of MNSF is mediated by specific cell surface membrane receptor protein. Identification of these receptors will provide insight into the apparently diverse activities of MNSF.
Third Division of Internal Medicine, Shimane Medical University, Izumo, Japan.
M
M
Nakamura
H
H
Ogawa
T
T
Tsunematsu
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology; D003208:Concanavalin A / Q000494:pharmacology; D006801:Humans; D007457:Iodine Radioisotopes; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D018121:Receptors, Cytokine; D011971:Receptors, Immunologic / Q000502:physiology*; D013045:Species Specificity; D013154:Spleen / Q000502:physiology; D013491:Suppressor Factors, Immunologic / Q000502:physiology*; D013601:T-Lymphocytes / Q000502:physiology
D007457:Iodine Radioisotopes; D008070:Lipopolysaccharides; D018121:Receptors, Cytokine; D011971:Receptors, Immunologic; D013491:Suppressor Factors, Immunologic; C066109:monoclonal-nonspecific suppressor factor receptor; D003208:Concanavalin A