Chemical and photochemical probing of DNA complexes.
An overview of the chemical and photochemical probes which over the past ten years have been used in studies of DNA/ligand complexes and of non-B-form DNA conformations is presented with emphasis on the chemical reactions of the probes with DNA and on their present 'use-profile'. The chemical probes include: dimethyl sulfate, ethyl nitroso urea, diethyl pyrocarbonate, osmium tetroxide, permanganate, aldehydes, methidiumpropyl-EDTA-Fell (MPE), phenanthroline metal complexes and EDTA/FeII. The photochemical probes that have been used include: psoralens, UVB, acridines and uranyl salts. The biological systems analysed by use of these probes are reviewed by tabulation.
Department of Biochemistry B, Panum Institute, University of Copenhagen, Denmark.
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Nielsen
D004247:DNA; D007202:Indicators and Reagents; D008958:Models, Molecular; D015335:Molecular Probes; D009690:Nucleic Acid Conformation; D010777:Photochemistry
D007202:Indicators and Reagents; D015335:Molecular Probes; D004247:DNA