Multilobated lymphoma of B cell type: a multiparameter investigation.
Multilobated lymphomas were originally described as T-cell neoplasms, but many of B-cell type have subsequently been reported. A case of B-cell origin is reported in which both immunophenotypic and genotypic studies performed on a cell suspension of the lymphoma gave inconclusive and potentially misleading information, while paraffin and frozen section immunohistologic studies, as well as genotypic studies performed on DNA obtained from snap-frozen tissue, were definitive. Thus, this case illustrates some of the problems that may be encountered using cell suspensions as a source for immunophenotypic, and even the much more sensitive genotypic, studies.
Westermann
C D
CD
University of Cincinnati College of Medicine, OH 45267-0529.
Steele
P E
PE
Hurtubise
P E
PE
Vago
J F
JF
Swerdlow
S H
SH
D015703:Antigens, CD / Q000276:immunology; D002469:Cell Separation / Q000379:methods; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D005434:Flow Cytometry; D005838:Genotype; D006651:Histocytochemistry; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D016393:Lymphoma, B-Cell / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D008875:Middle Aged
D015703:Antigens, CD; D004273:DNA, Neoplasm