Macromolecular release from collagen monolithic devices.
Collagen monolithic devices varying in crosslinking density, collagen structure, and crosslinker were fabricated. In vitro release rates of a model macromolecule, inulin, were found to be linear with t1/2 and were affected by crosslinking density, nature of crosslinker, and collagen structure. The biodegradation of the collagen matrix was also examined. Proteolytic enzymes did not degrade the collagen devices; the degradation rate with collagenase was dependent on collagen structure, crosslinker, crosslinking density, and enzyme concentration. In vivo biocompatibility, degradation, and 14C-inulin release rates were evaluated subcutaneously in rats. After 3 weeks, none of the collagen discs induced any severe cellular response. Dacron induced a stronger fibroblast response but fewer inflammatory cells as compared to the collagen discs. No significant degradation of the collagen discs occurred within 3 weeks. In vivo release of 14C-inulin from collagen monolithic devices was diffusion controlled.
Gilbert
D L
DL
Department of Pharmaceutics, University of Utah, Salt Lake City, 84108.
Kim
S W
SW
D000818:Animals; D003094:Collagen / Q000737:chemistry* / Q000493:pharmacokinetics / Q000648:ultrastructure; D003432:Cross-Linking Reagents; D003692:Delayed-Action Preparations; D007444:Inulin / Q000493:pharmacokinetics*; D008422:Materials Testing; D051381:Rats; D011919:Rats, Inbred Strains
D003432:Cross-Linking Reagents; D003692:Delayed-Action Preparations; D007444:Inulin; D003094:Collagen