Comparison of an enzyme-immunoassay with a radio-immunoassay method for the detection of the hepatitis markers anti-HBs, anti-HBc and HBsAg.
A comparative study was carried out on a radio-immunoassay (RIA) and enzyme-immunoassay (EIA) method for detecting the hepatitis markers anti-HBs, anti-HBc and HBsAg. The results indicated that the RIA and EIA were comparable for the HBsAg marker but that the RIA test was more sensitive for anti-HBs and more specific for anti-HBc. The conclusion was that if the EIA test is used for these markers, the laboratory and clinician must be aware of these limitations.
Blackburn
N K
NK
Department of Virology, University of the Witwatersrand, Johannesburg.
Schoub
B D
BD
O'Connell
K F
KF
D005069:Evaluation Studies as Topic; D006510:Hepatitis B Antibodies / Q000032:analysis*; D006512:Hepatitis B Core Antigens / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000032:analysis* / Q000276:immunology; D007124:Immunoenzyme Techniques; D011863:Radioimmunoassay
D006510:Hepatitis B Antibodies; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens