A chondrogenic cell line derived from a differentiating culture of AT805 teratocarcinoma cells.
A cell line, ATDC5, isolated from a differentiating culture of AT805 teratocarcinoma expressed a fibroblastic cell phenotype in a growing phase. With the addition of 10 micrograms/ml insulin to the medium, cells continued to grow even in a postconfluent phase, formed cartilage nodule-like cell aggregates, were stained with Alcian blue and produced cartilage-specific proteoglycan and type II collagen, typical marker molecules for chondrogenesis. Since ATDC5 cells also differentiated into unidentifiable pigmented cells, they are apparently composed of undetermined cells. ATDC5, therefore, provides a good model system with which to understand chondrogenic differentiation.
Laboratory of Molecular Oncology, Tsukuba Life Science Center, Ibaraki, Japan.
T
T
Atsumi
Y
Y
Miwa
K
K
Kimata
Y
Y
Ikawa
D000818:Animals; D002356:Cartilage / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D003094:Collagen / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology; D051379:Mice; D011509:Proteoglycans / Q000378:metabolism*; D013724:Teratoma
D007328:Insulin; D011509:Proteoglycans; D003094:Collagen