Inhibition of Trichomonas vaginalis motility by monoclonal antibodies is associated with reduced adherence to HeLa cell monolayers.
Adherence of trichomonads to host epithelial cells appears to be a critical step in the pathogenesis of trichomoniasis. We evaluated the effect of a panel of 10 monoclonal antibodies on attachment of [35S]methionine-radiolabeled Trichomonas vaginalis strains to HeLa cell monolayers. Of 10 monoclonal antibodies, 3 totally eliminated motility of PHS2J strain trichomonads and reduced their adherence to 48 to 60% of control values (P less than 0.001). However, none of the monoclonal antibodies affected motility or adherence of STD13 strain trichomonads. Although the antibodies all reacted with PHS2J trichomonads by immunofluorescence, there was no correlation between inhibition of adherence and findings on either immunofluorescence or radioimmunoprecipitation. Direct microscopic observations showed that incubation with the monoclonal antibodies did not cause cytolysis of T. vaginalis. In quantitative cultures there was no difference in the number of colonies produced by parasites that had been incubated with antibodies that inhibited or had no effect on adherence. We conclude that our monoclonal antibodies reduced adherence not by cytotoxic effects or by competing for specific sites mediating adherence of the protozoa, but by inhibiting motility of T. vaginalis.
Krieger
J N
JN
Department of Urology, University of Washington School of Medicine, Seattle 98195.
Torian
B E
BE
Hom
J
J
Tam
M R
MR
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000920:Antibody-Dependent Cell Cytotoxicity; D002448:Cell Adhesion; D004848:Epithelium / Q000469:parasitology; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells / Q000469:parasitology*; D006801:Humans; D009068:Movement; D011233:Precipitin Tests; D014245:Trichomonas Infections / Q000469:parasitology*; D014246:Trichomonas vaginalis / Q000276:immunology / Q000472:pathogenicity*
D000911:Antibodies, Monoclonal