[Increase in stability of the recombinant phage M13 carrying Escherichia coli genes rpIJL by reducing expression of cloned genes].
A recombinant phage mp9MW/rpoB containing the BglII-B fragment of the Escherichia coli rplJL-rpoBC gene cluster was constructed on the basis of filamentous phage M13. Stability of the phage was increased by insertion of a transcription terminator t beta' which blocked transcription of cloned genes from the Plac of the vector.
M I
MI
Vudmaska
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AN
Zhivolup
E B
EB
Paton
D003001:Cloning, Molecular; D003090:Coliphages / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D015967:Gene Expression Regulation, Viral; D005798:Genes, Bacterial; D005814:Genes, Viral; D010957:Plasmids; D011995:Recombination, Genetic
Povyshenie stabil'nosti rekombinantnogo faga M13, soderzhashchego geny rpIJL-rpoBC Escherichia coli, putem snizheniia ékspressii vstroennykh genov.