[Synthesis of immunoglobulin conjugates with dehydrogenases].
Three methods of synthesis of immunoglobulin conjugates with malate, lactate and glucose-6-phosphate dehydrogenases, involving the sodium metaperiodate oxidation of immunoglobulin carbohydrate component, use of water-soluble carbodiimide and the one-step glutaraldehyde technique, were compared. The glutaraldehyde method was shown to give immunoglobulin-dehydrogenase conjugates with high catalytic and immunochemical activity, which may be useful for enzyme-immunoassay.
Markina
V L
VL
Karaseva
E I
EI
Eremin
A N
AN
D055598:Chemical Phenomena; D002621:Chemistry; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000138:chemical synthesis*; D010088:Oxidoreductases / Q000138:chemical synthesis*
D007136:Immunoglobulins; D010088:Oxidoreductases
Sintez kon''iugatov immunoglobulinov s degidrogenazami.