Simultaneous determination of amino-alpha-carbolines and amino-gamma-carbolines in cigarette smoke condensate by high-performance liquid chromatography.
A method for the simultaneous detection of amino-alpha-carbolines (2-amino-alpha-carboline and 2-amino-3-methyl-alpha-carboline) and amino-gamma-carbolines (3-amino-1,4-dimethyl-5H-pyrido [4,3-b]indole and 3-amino-1-methyl-5H-pyrido [4,3-b]indole) by high-performance liquid chromatography has been developed. It consists of a three-step purification using three different columns with fluorometric detection. With this method, we have demonstrated that both amino-alpha-carbolines and amino-gamma-carbolines are present in cigarette smoke condensate. The method may be useful for detecting these carcinogens in various materials.
Manabe
S
S
Department of Hygiene and Preventive Medicine, Faculty of Medicine, University of Tokyo, Japan.
Wada
O
O
Kanai
Y
Y
D002243:Carbolines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D010947:Plants, Toxic; D012906:Smoke / Q000032:analysis*; D014026:Nicotiana / Q000032:analysis*
D002243:Carbolines; D012906:Smoke