Characterization of a mouse monoclonal antibody to vimentin by indirect immunofluorescence microscopy and immunoblotting.
A murine IgM monoclonal antibody, termed MC15A13G was produced after immunization of Balb/c mouse with Swiss mouse dental papillae. This antibody, characterized by immunoblotting analysis and indirect immunofluorescence microscopy, recognized a 57 Kda protein identified as vimentin in different cell types with no cross reaction with other cytoskeletal proteins.
Institut de Biologie Médicale, INSERM CJF 88-08, Université Louis Pasteur, Faculté de médecine, Strasbourg, France.
J L
JL
Fausser
H
H
Lesot
G
G
Zidan
J V
JV
Ruch
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D006224:Cricetinae; D003412:Cricetulus; D003771:Dental Papilla / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis; D051379:Mice; D014746:Vimentin / Q000032:analysis*
D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D014746:Vimentin