Tissue reaction to subcutaneous implantation of a collagen sponge. A histological, ultrastructural, and immunological study.
The biocompatibility of a subcutaneously implanted bovine collagen sponge (Haemostagen) was studied in rats by analyzing tissue reactions up to 3 months by histological and ultrastructural methods; in addition, the level of serum antibodies to bovine type I collagen (the major implant collagen) was measured by solid-phase radioimmunoassay. By 8 h after implantation, the implant was completely filled with polymorphonuclear cells (PMNs). After 8 days, fibroblasts had developed a granulation tissue within the sponge and the PMNs had almost disappeared. The small residue that remained after 1 month consisted of some densely packed collagen fibrils containing giant cells, which had disappeared by 3 months. No antibodies to bovine type I collagen were found in the sera of implanted rats.
Laboratoire d'Histologie Expérimentale, URA CNRS 244, Université Claude Bernard, Villeurbanne.
K
K
Anselme
C
C
Bacques
G
G
Charriere
D J
DJ
Hartmann
D
D
Herbage
R
R
Garrone
D000818:Animals; D001672:Biocompatible Materials; D003094:Collagen / Q000276:immunology; D005549:Foreign-Body Reaction / Q000276:immunology / Q000473:pathology*; D007249:Inflammation / Q000473:pathology; D008297:Male; D008422:Materials Testing; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D015503:Surgical Sponges / Q000009:adverse effects*
D001672:Biocompatible Materials; D003094:Collagen