Detection of platelet activation using activation specific monoclonal antibodies.
Platelets may become activated in a number of clinical disorders and participate in thrombus formation. Blood tests reflecting in vivo activation are therefore potentially useful in evaluating patients with thrombotic diseases. Three types of monoclonal antibodies have been described that react preferentially with activated platelets. Antibodies against a 53-kD lysosomal granule protein, and antibodies that recognize a 140-kD alpha-granule protein, are two types expressed on the platelet surface during secretion. A third type is not dependent on secretion and recognizes activation-dependent changes in the configuration or microenvironment of the platelet glycoprotein IIb/IIIa complex. Several procedures were used to detect platelet activation, using radiolabeled or fluorescent antibodies. In a number of disorders, changes in platelets, reflecting activation, could be detected. For the study of in vitro and in vivo platelet activation, these tests may be useful, but further studies are needed to confirm the power and efficiency of this approach compared to other routine tests.
Department of Haematology, University Hospital Utrecht, The Netherlands.
M J
MJ
Metzelaar
J J
JJ
Sixma
H K
HK
Nieuwenhuis
D000911:Antibodies, Monoclonal; D006801:Humans; D015539:Platelet Activation / Q000276:immunology*
D000911:Antibodies, Monoclonal