The DNA binding subunit of NF-kappa B is identical to factor KBF1 and homologous to the rel oncogene product.
The major determinant in the transcriptional control of class I genes of the major histocompatibility complex is an enhancer sequence located around -170 from the transcription start site, which binds a factor named KBF1. We have isolated a complementary cDNA coding for KBF1 and identified the DNA binding and dimerization domain of the protein. Because KBF1 and the transcription factor NF-kappa B bind to similar sequences, we investigated the relationship between these two molecules. It appeared that KBF1 is, by all criteria used, identical to the 50 kd DNA binding subunit of NF-kappa B. KBF1 (and therefore p50) also displays extensive amino acid sequence homology with the v-rel oncogene and the Drosophila maternal morphogen dorsal. In vitro experiments suggest functional homologies between KBF1 and v-rel.
Unité de Biologie Moléculaire du Gène, U.277 INSERM, U.A.C. 115 CNRS, Institut Pasteur, Paris, France.
M
M
Kieran
V
V
Blank
F
F
Logeat
J
J
Vandekerckhove
F
F
Lottspeich
O
O
Le Bail
M B
MB
Urban
P
P
Kourilsky
P A
PA
Baeuerle
A
A
Israël
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004330:Drosophila / Q000235:genetics; D004742:Enhancer Elements, Genetic; D005260:Female; D015723:Gene Library; D005805:Genes, MHC Class I; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D016328:NF-kappa B; D052002:NF-kappa B p50 Subunit; D020572:Oncogene Proteins v-rel; D009857:Oncogenes; D010957:Plasmids; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D046911:Macromolecular Substances; D016328:NF-kappa B; D052002:NF-kappa B p50 Subunit; C489548:NFKB1 protein, human; D020572:Oncogene Proteins v-rel; D015682:Retroviridae Proteins, Oncogenic; D014157:Transcription Factors; D011505:Protein-Tyrosine Kinases