Production of interleukin 1 from human monocytes stimulated by synthetic lipid A subunit analogues.
We have investigated that synthetic lipid A subunit analogues (GLA compounds) as well as E. coli type lipopolysaccharide (LPS) and synthetic lipid A (compound 506) are able to stimulate human monocytes to release IL-1 in vitro. Of monosaccharide-type GLA compounds, GLA-60 was found to be more active for the induction of IL-1 production than GLA-59 and GLA-27, and similar to that of LPS or compound 506. GLA-60 could induce not only the secretion of IL-1 into culture supernatant but also the expression of membrane-associated form of IL-1 in human monocytes. Furthermore, no detectable IL-2 activity was observed in the culture supernatant. These results show that synthetic lipid A analogues of low toxicity, in particular GLA-60, are active in inducing IL-1 production in human monocytes.
Institute of Immunological Science, Hokkaido University, Sapporo, Japan.
I
I
Saiki
H
H
Maeda
J
J
Murata
T
T
Takahashi
S
S
Sekiguchi
M
M
Kiso
A
A
Hasegawa
I
I
Azuma
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects; D003470:Culture Media / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008050:Lipid A / Q000031:analogs & derivatives* / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology
D003470:Culture Media; D007375:Interleukin-1; D008050:Lipid A; D008070:Lipopolysaccharides; C056260:GLA 60