[Chemical neuro-anatomy of the thalamus: an example of the co-expression of 2 neuropeptides, cholecystokinin and intestinal vasoactive peptide].
Recent studies using hybridization histochemistry have demonstrated the presence of cholecystokinin (CCK) gene expression in thalamocortical and thalamo-striatal neurons. To further understand the chemical anatomy of the thalamus, we used the same method to study the coexpression of CCK and vasoactive intestinal peptide (VIP) genes in the same neurons. Most of the neurons expressing the VIP gene in the rat dorsal thalamus (found especially in the ventrolateral nucleus) also expressed the CCK gene, as demonstrated by autoradiography on emulsion-coated adjacent sections studied with probes recognizing either mRNA. Some further neurons, located in the reticular nucleus, had VIP mRNA at lower levels of expression but did not appear to contain CCK mRNA.
Burgunder
J M
JM
Neurologische Klinik, Inselspital Bern.
D000818:Animals; D001345:Autoradiography; D002766:Cholecystokinin / Q000235:genetics*; D015342:DNA Probes; D015870:Gene Expression; D008297:Male; D009474:Neurons / Q000032:analysis; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D013788:Thalamus / Q000032:analysis*; D014660:Vasoactive Intestinal Peptide / Q000235:genetics*
D015342:DNA Probes; D012333:RNA, Messenger; D014660:Vasoactive Intestinal Peptide; D002766:Cholecystokinin
La neuroanatomie chimique du thalamus: exemple de coexpression de deux neuropeptides, cholécystokinine et peptide vasoactif intestinal.