Sequence and chromosomal location of the I-309 gene. Relationship to genes encoding a family of inflammatory cytokines.
We previously reported the isolation and characterization of a cDNA clone, I-309, that encodes a small secreted protein produced by activated human T lymphocytes. This protein is structurally related to a large number of recently identified proteins that are secreted upon cellular activation. In this report we describe the isolation and characterization of the gene encoding I-309. The genomic organization is essentially identical to that found in the genes encoding the structurally similar proteins TCA-3, hJE/MCP-1, and mJE, strengthening the hypothesis that these genes are evolutionarily related. The region of the I-309 gene 5' of the mRNA cap site exhibits extensive nucleotide sequence homology with the same region of the murine gene TCA-3, providing additional evidence that I-309 and TCA-3 are likely to be homologs. Finally, panels of rodent-human somatic cell hybrids were used to map the I-309 gene to human chromosome 17. In conjunction with recent mapping data from other laboratories, this result suggests the presence of a cluster of related genes on this chromosome.
Miller
M D
MD
Division of Tumor Virology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115.
Wilson
S D
SD
Dorf
M E
ME
Seuanez
H N
HN
O'Brien
S J
SJ
Krangel
M S
MS
D000595:Amino Acid Sequence; D001483:Base Sequence; D054402:Chemokine CCL1; D019742:Chemokines, CC; D002630:Chemotactic Factors / Q000235:genetics*; D002886:Chromosomes, Human, Pair 17; D003001:Cloning, Molecular; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic
C065971:CCL1 protein, human; D054402:Chemokine CCL1; D019742:Chemokines, CC; D002630:Chemotactic Factors