The application of enhanced chemiluminescence to membrane-based nucleic acid detection.
The ECL gene detection system is a novel, sensitive, non-radioactive system for the detection of nucleic acid hybridized on both nylon and nitrocellulose membranes. It is characterized by direct labeling of probe sequences with horseradish peroxidase combined with an enhanced chemiluminescent (ECL) detection reaction; the light output is captured on blue-light sensitive film. The application of the system to a range of standard molecular biology hybridization techniques is described.
Durrant
I
I
Amersham International plc., Amersham, Bucks, U.K.
Benge
L C
LC
Sturrock
C
C
Devenish
A T
AT
Howe
R
R
Roe
S
S
Moore
M
M
Scozzafava
G
G
Proudfoot
L M
LM
Richardson
T C
TC
D015152:Blotting, Northern; D015139:Blotting, Southern; D011905:Genes, ras; D006735:Horseradish Peroxidase; D008163:Luminescent Measurements; D009101:Multiple Myeloma / Q000235:genetics; D009693:Nucleic Acid Hybridization; D015341:Nucleic Acid Probes; D009696:Nucleic Acids / Q000032:analysis*
D015341:Nucleic Acid Probes; D009696:Nucleic Acids; D006735:Horseradish Peroxidase