A sensitive sandwich enzyme immunoassay for measurement of insulin on microtiter plates.
A sensitive enzyme immunoassay for the measurement of insulin in human sera on microtiter plates was established. The assay is based on the sandwich technique with guinea pig anti-insulin IgG adsorbed at microtiter plate wells, human insulin as standard and the same anti-insulin IgG labeled with horseradish peroxidase. Standards used cover a range from 0 to 1200 pmol/l with a detection limit of 10 pmol/l. Coefficients of variation between 3-7% for intraassay precision and 5-11% for interassay precision were obtained over the concentration range of 80-1000 pmol/l. The correlation of EIA-data with those of a commercially available double antibody radioimmunoassay (r = 0.98) could be expressed by the equation: EIA = 0.97 RIA - 57 pmol/l. Normal fasting serum insulin concentrations in healthy subjects ranged from 11-165 pmol/l. In subjects with potentially diminished basal values concentrations of 10-79 pmol/l were determined. The insulin response in oral glucose tolerance tests of children was discussed, who had a constitutional tall stature or Turner's syndrome, respectively.
Institute of Clinical Chemistry and Laboratory Diagnosis, Karl-Marx-University Medical School Leipzig, GDR.
J
J
Kratzsch
W
W
Ackermann
H
H
Keilacker
W
W
Besch
E
E
Keller
D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D005951:Glucose Tolerance Test; D006801:Humans; D007124:Immunoenzyme Techniques; D007231:Infant, Newborn / Q000378:metabolism; D007328:Insulin / Q000032:analysis*; D008875:Middle Aged; D014424:Turner Syndrome / Q000378:metabolism
D001786:Blood Glucose; D007328:Insulin