The mutagenic activity of ethylmethanesulphonate, benzidine and benzo[a]pyrene at the hprt locus of wild-type L5178Y mouse lymphoma cells.
Ethylmethanesulphonate (EMS), benzidine (BZD) and benzo[a]pyrene (B[a]P) were assayed for ability to induce mutation at the hprt locus of wild-type L5178Y mouse lymphoma cells. EMS was assayed in the absence of metabolic activation, B[a]P in the presence of metabolic activation (S-9 mix) and BZD both in the absence and presence of S-9. Treatment with EMS minus S-9 and B[a]P plus S-9, especially when the S-9 content of the incubation was 2% (v/v), produced strong dose-related increases in mutant frequency. BZD failed to induce mutation at the hprt locus, either in the absence or presence of S-9.
Kennelly
J C
JC
Microtest Research Ltd., Heslington, York, UK.
Clare
C B
CB
Campbell
J
J
Lane
M P
MP
Harrington
D H
DH
Cole
H
H
Garner
R C
RC
D000818:Animals; D001560:Benzidines / Q000378:metabolism / Q000633:toxicity*; D001564:Benzo(a)pyrene / Q000378:metabolism / Q000633:toxicity*; D001711:Biotransformation; D002470:Cell Survival / Q000187:drug effects; D005020:Ethyl Methanesulfonate / Q000633:toxicity*; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D007940:Leukemia L5178; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000378:metabolism / Q000633:toxicity*; D009154:Mutation; D014407:Tumor Cells, Cultured
D001560:Benzidines; D009153:Mutagens; C029876:benzidine; D001564:Benzo(a)pyrene; D005020:Ethyl Methanesulfonate; D007041:Hypoxanthine Phosphoribosyltransferase