A simple procedure for demonstrating the overall morphology of fibroblasts in routine histological preparations of adult tissues, using silver impregnation.
A silver impregnation procedure for the total visualization of fibroblasts, with all their processes, is described. The method is applicable to routine formalin-fixed paraffin sections, or to formalin-fixed or native cryostat sections. The results are illustrated with examples from loose and dense connective tissue. Fibroblasts are visualized as cells with long, branching dendritic processes. The fibroblasts maintain contact with each other via these processes, thus forming a reticulum of cells, even in adult connective tissue.
Abt. Neuroanatomie, Universität Düsseldorf, F.R.G.
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GE
Novotny
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Gommert-Novotny
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002648:Child; D002675:Child, Preschool; D003239:Connective Tissue Cells; D005347:Fibroblasts / Q000166:cytology*; D006652:Histological Techniques; D006801:Humans; D007223:Infant; D008168:Lung / Q000166:cytology; D008251:Macaca; D008853:Microscopy / Q000379:methods; D008875:Middle Aged; D051381:Rats; D012834:Silver; D012867:Skin / Q000166:cytology; D013154:Spleen / Q000166:cytology
D012834:Silver