A microplate method for the determination of amino groups in monoclonal antibodies.
The total number of amino groups in monoclonal antibodies can be estimated via a colorimetric assay using trinitrobenzene sulfonic acid (A.F.S.A. Habeeb (1966) Anal. Biochem. 14, 328-336). This work describes the adaptation of this method to a microtiter plate in order to rapidly and reliably assay large numbers of samples.
Biotherapeutics, Inc., Franklin, TN.
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Law
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Ahmad
D000588:Amines / Q000032:analysis*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D051379:Mice; D008846:Micromanipulation; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000379:methods; D013997:Time Factors
D000588:Amines; D000911:Antibodies, Monoclonal