Chinese hamster ovary cell mutants defective in myo-inositol transport.
By means of an in situ colony autoradiographic assay for the incorporation of [14C]inositol into the trichloroacetic acid-insoluble fraction, we have isolated a mutant of cultured Chinese hamster ovary cells defective in inositol transport, named mutant 648. Through comparison of the inositol uptake activity of 648 cells with that of the parental cells with various concentrations of inositol and sodium, it has been demonstrated that Chinese hamster ovary cells possess a sodium-dependent transport system for inositol, and that 648 cells lack this system. The sodium-dependent uptake is inhibited by 2,4-dinitrophenol and ouabain, and the intracellular concentration of inositol exceeds the extracellular concentration during the uptake period, indicating that it is active transport, at least partially driven by the sodium gradient generated by Na+,K(+)-ATPase. The apparent Km for inositol has been estimated to be 12.0 microM. It is inhibited by hyperglycemic concentration of D-glucose in a competitive fashion.
Department of Chemistry, National Institute of Health, Tokyo, Japan.
Y
Y
Ohga
M
M
Nishijima
Y
Y
Akamatsu
D000818:Animals; D001693:Biological Transport, Active; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D007294:Inositol / Q000378:metabolism*; D009154:Mutation; D010053:Ovary; D012964:Sodium / Q000502:physiology*; D013997:Time Factors
D007294:Inositol; D012964:Sodium