The expression of granulocyte/macrophage colony-stimulating factor in activated mouse lymphocytes declines with age.
The expression of granulocyte/macrophage colony-stimulating factor (GM-CSF) was studied in spleen lymphocytes isolated from male C57BL/6J mice of 6, 20, and 29 months of age. GM-CSF expression (biological activity and mRNA level) was maximum after culturing the lymphocytes for 45 hr with concanavalin A and phorbol myristate acetate. The induction of both GM-CSF activity and mRNA levels was observed to decline over 60% between 6 and 29 months of age. The age-related decline in the level of GM-CSF paralleled the age-related decline in the mRNA levels of interleukin-2 and interleukin-3.
Department of Biological Sciences, Illinois State University, Normal 61761.
N S
NS
Cai
D D
DD
Li
H T
HT
Cheung
A
A
Richardson
D000375:Aging / Q000276:immunology*; D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000378:metabolism
D007376:Interleukin-2; D012333:RNA, Messenger; D003208:Concanavalin A; D016178:Granulocyte-Macrophage Colony-Stimulating Factor