Antibody exchange immunochemistry.
Antibodies are found to transfer rapidly between antigen samples attached to separate solid supports. The half-times for this antibody exchange at 20 and 37 degrees C were 7 and 1 h, respectively. Antibody exchange was exhibited by all 17 polyclonal and monoclonal antibodies tested and was readily detected by enzyme-linked immunosorbent assay, immunoblotting, or immunocytochemical assays. Taking advantage of this phenomenon, antibodies can be affinity-purified using less than a picomole of antigen and small amounts of antibody.
Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545.
J A
JA
Hammarback
R B
RB
Vallee
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D001923:Brain Chemistry; D002417:Cattle; D002460:Cell Line; D003600:Cytosol / Q000032:analysis; D007150:Immunohistochemistry; D007158:Immunologic Techniques; D007700:Kinetics; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000276:immunology*; D008870:Microtubules / Q000648:ultrastructure; D014404:Tubulin / Q000032:analysis / Q000276:immunology
D000911:Antibodies, Monoclonal; D008869:Microtubule-Associated Proteins; D014404:Tubulin