Cloning and characterization of cDNAs for murine macrophage inflammatory protein 2 and its human homologues.
A cDNA clone of murine macrophage inflammatory protein 2 (MIP-2) has been isolated from a library prepared from lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and the nucleotide sequence determined. This cDNA was used to clone cDNAs for human homologues of MIP-2 from a library prepared from phorbol myristate acetate-treated and LPS-stimulated U937 cells. Two homologues were isolated and sequenced. Human MIP-2 alpha and MIP-2 beta are highly homologous to each other and to a previously isolated gene, human gro/melanoma growth-stimulating activity (MGSA). These three human genes, MIP-2 alpha, MIP-2 beta, and gro/MGSA, constitute a sub-family within the cytokine family represented by platelet factor 4 and interleukin 8.
Chiron Corporation, Emeryville, California 94608.
P
P
Tekamp-Olson
C
C
Gallegos
D
D
Bauer
J
J
McClain
B
B
Sherry
M
M
Fabre
S
S
van Deventer
A
A
Cerami
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D002460:Cell Line; D054426:Chemokine CXCL2; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015723:Gene Library; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8; D051379:Mice; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012689:Sequence Homology, Nucleic Acid
C499706:CXCL2 protein, human; C499707:CXCL3 protein, human; D002352:Carrier Proteins; D054426:Chemokine CXCL2; D019743:Chemokines, CXC; D002630:Chemotactic Factors; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8; D015345:Oligonucleotide Probes; D004247:DNA