The calcium-binding site of clathrin light chains.
Clathrin light chains are calcium-binding proteins (Mooibroek, M. J., Michiel, D. F., and Wang, J. H. (1987) J. Biol. Chem. 262, 25-28) and clathrin assembly can be modulated by calcium in vitro. Thus, intracellular calcium may play a regulatory role in the function of clathrin-coated vesicles. The structural basis for calcium's influence on clathrin-mediated processes has been defined using recombinant deletion mutants and isolated fragments of the light chains. A single calcium-binding site, formed by residues 85-96, is present in both mammalian light chains (LCa and LCb) and in the single yeast light chain. This sequence has structural similarity to the calcium-binding EF-hand loops of calmodulin and related proteins. In mammalian light chains, the calcium-binding sequence is flanked by domains that regulate clathrin assembly and disassembly.
Näthke
I
I
Department of Pharmacy, School of Pharmacy, University of California, San Francisco 94143.
Hill
B L
BL
Parham
P
P
Brodsky
F M
FM
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000648:ultrastructure; D002417:Cattle; D002966:Clathrin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D003196:Computer Graphics; D003488:Cyanogen Bromide / Q000737:chemistry; D004252:DNA Mutational Analysis; D066298:In Vitro Techniques; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism; D011994:Recombinant Proteins
D000911:Antibodies, Monoclonal; D002135:Calcium-Binding Proteins; D002966:Clathrin; D010446:Peptide Fragments; D011994:Recombinant Proteins; D003488:Cyanogen Bromide; D002118:Calcium