[The in vitro effect of a combination of sulbactam with cephalothin and cefazolin on strains of Escherichia coli and Proteus mirabilis which produce beta lactamase].
The effect of the combination of sulbactam, cephalothin, and cephazolin was studied in vitro on 88 strains of E. coli and 11 strains of P. mirabilis producing beta-lactamases. Quantitative susceptibility of the tested strains to cephalothin was examined by the disk dilution method and the effect of the combinations with sulbactam by the micromethod. The production of beta-lactamases was determined by the qualitative test with the chromogenic substrate PODAC. Of the total number of 160 E. coli strains beta-lactamases were established in 53.3% of the strains. Of the 47 P. mirabilis strains 23.4% were found to produce beta-lactamases. The effect of the combinations with sulbactam was assessed as synergistic, additive, indifferent or antagonistic. In the combination of sulbactam in the concentration of 16.0 mg/l with cephalothin and cephazolin a higher rate of synergistic and additive effect was recorded on both strains. In none of the combinations did sulbactam exert an antagonistic effect.
Výskumny ustav lieciv, Horna 36, Modra.
M
M
Múcková
P
P
Milosovic
D002437:Cefazolin / Q000008:administration & dosage / Q000494:pharmacology*; D002512:Cephalothin / Q000008:administration & dosage / Q000494:pharmacology*; D004338:Drug Combinations; D004357:Drug Synergism; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology; D008826:Microbial Sensitivity Tests; D011513:Proteus mirabilis / Q000187:drug effects* / Q000201:enzymology; D013407:Sulbactam / Q000008:administration & dosage / Q000494:pharmacology*; D001618:beta-Lactamases / Q000096:biosynthesis*
D004338:Drug Combinations; D001618:beta-Lactamases; D002437:Cefazolin; D002512:Cephalothin; D013407:Sulbactam
Ucinok kombinácií sulbaktámu s cefalotínom a cefazolínom in vitro na kmene Escherichia coli a Proteus mirabilis produkujúce betalaktamázy.