E. coli antibodies do not cause false-positivity in recombinant anti-HIV assays.
129 sera with known antibody titres against E. coli 026 and E. coli 055 strains were tested with the Abbot second generation anti-HTLV III recombinant screening assay. No difference in the O.D. values was found between sera with high, normal and low anti-E. coli titres. In addition, no false-positive reactions were observed with the anti-HIV negative sera containing E. coli antibodies in high titres in a Western blot assay in which recombinant env antigen was applied. These results suggest that E. coli assays in which E. coli-produced recombinant antigens are used.
Ujhelyi
E
E
National Institute of Haematology and Blood Transfusion, Budapest, Hungary.
Illei
G
G
Böhm
U
U
Füst
G
G
Dierich
M M
MM
Szelényi
J
J
Merétey
K
K
Hofbauer
J
J
Hollán
S R
SR
D000907:Antibodies, Bacterial / Q000276:immunology*; D001782:Blood Donors; D015153:Blotting, Western; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology*; D005189:False Positive Reactions; D015483:HIV Antibodies / Q000032:analysis*; D015488:HIV Antigens / Q000276:immunology; D006801:Humans; D011994:Recombinant Proteins / Q000276:immunology
D000907:Antibodies, Bacterial; D015483:HIV Antibodies; D015488:HIV Antigens; D011994:Recombinant Proteins