The regulatory effects of macrophages on colony stimulating factor-1 (CSF-1) production.
1. By using the anti-sense oligodeoxynucleotide inhibition strategy, we demonstrated that the production of CSF-1 by macrophage was blocked specifically by a CSF-1 anti-sense oligodeoxynucleotide complementary to the 5' end of the coding region of mouse CSF-1 m-RNA transcripts, but not by the CSF-1 mRNA transcripts, but not by the CSF-1 sense oligomer. 2. The LPS-induced condition medium of macrophages treated with CSF-1 anti-sense oligodeoxynucleotide retained the capacity of stimulating mouse fibroblast cells and bone marrow cells producing CSF-1 in vitro. 3. In addition to the inhibition of CSF-1 production by macrophages CSF-1 anti-sense oligodeoxynucleotide also inhibited the CSF-1 production of fibroblast cells and bone marrow cells stimulated by the macrophages conditioned medium. 4. These results suggest that macrophages stimulate their own development by mediating CSF-1 production at least in three ways: (1) production CSF-1; (2) stimulating fibroblast cells; (3) bone marrow cells to produce CSF-1. There is a positive feedback mechanism for macrophage hematopoiesis and its functional expression.
Department of Biochemistry, Nanjing University, People's Republic of China.
J
J
Wu
J Q
JQ
Zhu
D X
DX
Zhu
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism; D001854:Bone Marrow Cells; D002460:Cell Line; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000096:biosynthesis* / Q000235:genetics; D003470:Culture Media; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D007106:Immune Sera / Q000494:pharmacology; D007375:Interleukin-1 / Q000276:immunology / Q000502:physiology; D008070:Lipopolysaccharides / Q000494:pharmacology; D016173:Macrophage Colony-Stimulating Factor; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009841:Oligonucleotides / Q000494:pharmacology; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger
D003115:Colony-Stimulating Factors; D003470:Culture Media; D007106:Immune Sera; D007375:Interleukin-1; D008070:Lipopolysaccharides; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D016173:Macrophage Colony-Stimulating Factor