NMR studies of a bacterial cell culture medium (LB broth): cyclic nucleotides in yeast extracts.
The composition of LB broth (tryptone, yeast extract and NaCl) was investigated by 1H,31P-NMR spectroscopy, FPLC and gel electrophoresis. An unexpected finding was the high level of 2'3'-cyclic nucleotides, detected by characteristic 31P-NMR resonances in the region 20-21 ppm, originating from the yeast component. 31P-NMR resonances for cyclic nucleotides were observed during the autolysis of Saccharomyces cerevisiae cells, and in model reactions of RNase with RNA.
Department of Chemistry, Birkbeck College, Gordon House, Laboratory, London, U.K.
M H
MH
Rayner
P J
PJ
Sadler
M D
MD
Scawen
D001431:Bacteriological Techniques; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000032:analysis*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D009712:Nucleotides, Cyclic / Q000378:metabolism; D012331:RNA, Fungal / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism
D003470:Culture Media; D009712:Nucleotides, Cyclic; D012331:RNA, Fungal