[Synthesis and secretion of recombinant protein--the product of v-sis oncogene in Saccharomyces cerevisiae cells].
The recombinant plasmid DNA YEp secl-v-sis was constructed. This plasmid was able to code for the synthesis and secretion into the cultural medium of the protein-product of oncogene v-sis. Transcription, copy number and stability of the plasmid DNA were studied under the conditions of galactose induction. The v-sis protein was determined by gel electrophoresis and immunoblotting methods and assayed for cell-proliferation activity in the mammalian cell culture.
Ratovitskiĭ
E A
EA
Rudenko
A B
AB
Vasinova
N A
NA
Kidgotko
O V
OV
Nisman
B Kh
BKh
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D020575:Oncogene Proteins v-sis; D009857:Oncogenes; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D015183:Restriction Mapping; D015682:Retroviridae Proteins, Oncogenic / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D015291:Transforming Growth Factors / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
D020575:Oncogene Proteins v-sis; D011994:Recombinant Proteins; D015682:Retroviridae Proteins, Oncogenic; D015291:Transforming Growth Factors
Sintez i sekretsiia rekombinantnogo belka--produkta onkogena v-sis v kletkakh drozhzheĭ Saccharomyces cerevisiae.