Determination of benzydamine and its N-oxide in biological fluids by high-performance liquid chromatography.
A simple, sensitive and selective method for the determination of benzydamine in human plasma and urine, and for benzydamine N-oxide in urine, has been developed using high-performance liquid chromatography in the reversed-phase mode. The limit of reliable determination of benzydamine in plasma was 0.5 ng/ml and that in urine 1 ng/ml; the limit of reliable determination of benzydamine N-oxide in urine was 50 ng/ml. The method has been successfully applied to the analysis of these compounds in biological fluids after administration of intravenous and oral doses of benzydamine to human volunteers.
Baldock
G A
GA
Department of Metabolism and Pharmacokinetics, Huntingdon Research Centre, Cambridgeshire, U.K.
Brodie
R R
RR
Chasseaud
L F
LF
Taylor
T
T
D001591:Benzydamine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D006801:Humans
C048872:benzydamine N-oxide; D001591:Benzydamine