Attachment of epiphyseal cartilage cells and 17/28 rat osteosarcoma osteoblasts using mussel adhesive protein.
These experiments show that mussel adhesive protein (MAP) enhances the attachment of osteoblasts and epiphyseal cartilage cells to plastic culture dishes and Vitallium. When MAP was applied to culture plate surfaces, there were two- to fivefold increases in the numbers of cells attaching compared to control surfaces (no MAP). Results were confirmed using two different cell attachment assay techniques. Osteoblast replication and culture on MAP is possible, suggesting that MAP is not toxic to cells. MAP also holds applied cells to surfaces as initially attached.
Department of Orthopaedic Surgery, University of Connecticut School of Medicine, Farmington 06032.
J P
JP
Fulkerson
L A
LA
Norton
G
G
Gronowicz
P
P
Picciano
J M
JM
Massicotte
C W
CW
Nissen
D000818:Animals; D049872:Bivalvia; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division; D003584:Cytological Techniques / Q000295:instrumentation; D006132:Growth Plate / Q000166:cytology / Q000502:physiology*; D007930:Leucine; D010006:Osteoblasts / Q000502:physiology*; D012516:Osteosarcoma; D010969:Plastics; D011506:Proteins / Q000494:pharmacology*; D051381:Rats; D014407:Tumor Cells, Cultured
D010969:Plastics; D011506:Proteins; C037403:adhesive protein, mussel; D007930:Leucine