SPT6, an essential gene that affects transcription in Saccharomyces cerevisiae, encodes a nuclear protein with an extremely acidic amino terminus.
SPT6 is an essential gene of Saccharomyces cerevisiae that appears to play a role in transcription. Mutations in the SPT6 (SSN20, CRE2) gene suppress delta insertion mutations in the 5' regions of HIS4 and LYS2 and mutations in cis- and/or trans-acting elements that are required for expression of SUC2 and ADH2. We report here that SPT6 encodes a 170-kilodalton highly charged protein with an extremely acidic amino terminus. By use of an epitope-tagged SPT6 protein, we have determined by indirect immunofluorescence that the SPT6 protein is located in the nucleus.
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
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Swanson
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Carlson
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Winston
D000595:Amino Acid Sequence; D001483:Base Sequence; D005455:Fluorescent Antibody Technique; D005656:Fungal Proteins / Q000032:analysis / Q000235:genetics*; D005800:Genes, Fungal; D005838:Genotype; D056488:Histone Chaperones; D008969:Molecular Sequence Data; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D013489:Suppression, Genetic; D014158:Transcription, Genetic; D035602:Transcriptional Elongation Factors
D005656:Fungal Proteins; D056488:Histone Chaperones; D009687:Nuclear Proteins; C065456:SPT6 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D035602:Transcriptional Elongation Factors