Analysis of the THR4 region on chromosome III of the yeast Saccharomyces cerevisiae.
The gene encoding threonine synthase (THR4) from the yeast Saccharomyces cerevisiae was cloned by complementation of a thr4 mutant. This gene was also found on a lambda clone (5239) consisting of a fragment of chromosome III inserted in the vector lambdaMG3. The THR4 gene encodes a protein of 514 amino acids (M.W. 58 kDa), which has extensive homologies with E. coli threonine synthase (thrC) and B subtilis threonine synthase. The 5' flanking region of the gene contains three regulatory sequences [TGACT(C)] for the general amino acid control (GCN). About 130 bp downstream of the THR4 gene another large open reading frame (563 amino acids) is found in the opposite orientation. This may imply that this open reading frame, called CTR86, shares a terminator region with THR4. The function of the protein encoded by CTR86 is not yet clear, but the fact that the upstream region contains a GCN4 responsive site suggests that the gene product may also be involved in amino acid biosynthesis.
Institut für Physiologische Chemie, Universität München, F.R.G.
G
G
Mannhaupt
G
G
van der Linden
I
I
Vetter
K
K
Maurer
U
U
Pilz
R
R
Planta
H
H
Feldmann
D000595:Amino Acid Sequence; D001483:Base Sequence; D019757:Carbon-Oxygen Lyases; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D004271:DNA, Fungal / Q000235:genetics*; D005816:Genetic Complementation Test; D008190:Lyases / Q000235:genetics*; D008969:Molecular Sequence Data; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D004271:DNA, Fungal; D008190:Lyases; D019757:Carbon-Oxygen Lyases; C023160:threonine synthase