Functional expression of the mutants of the chloroplast tRNA(Lys) gene from the liverwort, Marchantia polymorpha, in Escherichia coli.
The anticodon of the tRNA(Lys) gene (trnK) in the liverwort, Marchantia polymorpha, was artificially converted to an amber anticodon. This mutant tRNA(Lys) (CTA) gene carrying either the intron of the C27-C43 mismatch at the anticodon-stem is not functional in Escherichia coli, but without both of them, it does work as a tRNA(Lys) amber suppressor.
Department of Biophysics, Faculty of Science, Kyoto University, Japan.
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Nakahigashi
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Inokuchi
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Ozeki
D001482:Base Composition; D001483:Base Sequence; D002736:Chloroplasts / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005091:Exons; D015870:Gene Expression; D017343:Genes, Plant; D007438:Introns; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D015345:Oligonucleotide Probes; D010944:Plants / Q000235:genetics*; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012357:RNA, Transfer, Lys / Q000235:genetics*; D013489:Suppression, Genetic
D015345:Oligonucleotide Probes; D012345:RNA, Transfer, Amino Acid-Specific; D012357:RNA, Transfer, Lys