2-Hydroxyethylnitrosourea induction of B cell lymphoma in female Swiss mice.
Groups of 30 adult Swiss mice of both sexes from the Eppley Institute breeding colony were treated with 37.5 or 75 mg 2-hydroxyethylnitrosourea (HENU)/l citrate buffer, given as drinking water on 4 days/week from 6-8 weeks of age for life. A third group of mice was untreated. Incidences of generalized B cell lymphoma (BCL) were 67% (males) and 87% (females) for the higher dose of the nitrosourea, 29% (males) and 79% (females) for the lower dose and 0% (males) and 7% (females) for the untreated mice. Tumor latencies were 23 weeks (high dose) and 27 weeks (low dose) in the females and 6-7 weeks longer in the males. A B-cell origin of the lymphomas was indicated by the pathology and tumor distribution and was confirmed in 4 mice by immunophenotyping and in 6 mice by probes for gene rearrangements. This system may be an effective means of inducing B cell lymphoma with a carcinogen.
Eppley Institute for Research in Cancer, University of Nebraska Medical Center, Omaha 68198.
S S
SS
Mirvish
D D
DD
Weisenburger
S S
SS
Joshi
J
J
Nickols
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005038:Ethylnitrosourea / Q000031:analogs & derivatives* / Q000633:toxicity; D005260:Female; D015321:Gene Rearrangement; D008198:Lymph Nodes / Q000473:pathology; D016393:Lymphoma, B-Cell / Q000139:chemically induced* / Q000235:genetics / Q000473:pathology; D008297:Male; D051379:Mice; D012737:Sex Factors; D013154:Spleen / Q000473:pathology; D013997:Time Factors
C022146:1-(2-hydroxyethyl)-1-nitrosourea; D005038:Ethylnitrosourea