Comparison of two sperm preparation techniques using automated sperm motion analysis: migration sedimentation versus swim-up.
Migration sedimentation and spermatozoa swim-up techniques were used for obtaining spermatozoa from the semen samples of 39 infertile men. Concentration, percentage of motile sperm, velocity, linearity, and motility index of the sperm preparations obtained by both methods were compared using the CellSoft automated sperm motion analyzer. The mean velocity of the spermatozoa obtained after the migration sedimentation technique was significantly higher than that with swim-up technique. Since it is not necessary to centrifuge spermatozoa with the migration sedimentation technique, this method may be more desirable than other techniques using centrifugation.
Yener
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C
Department of Obstetrics and Gynecology, Medical University of South Carolina, Charleston.
Mathur
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S
Parent
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B
D003936:Diagnosis, Computer-Assisted / Q000379:methods*; D006801:Humans; D008297:Male; D013076:Sperm Count; D013081:Sperm Motility