Negative-strand RNA replication by Q beta and MS2 positive-strand RNA phage replicases.
Plasmids were constructed which allowed preparation of positive- and negative-strand MS2 RNA by in vitro transcription and which expressed the MS2 phage replicase. Transcribed, positive-strand MS2 RNA was infectious to Escherichia coli spheroplasts. Transcribed, negative-strand MS2 RNA was infectious to spheroplasts containing either MS2 replicase or related bacteriophage Q beta replicase.
Department of Biochemistry, Texas College of Osteopathic Medicine/University of North Texas, Fort Worth 76107.
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Shaklee
D001435:Bacteriophages / Q000201:enzymology / Q000235:genetics; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics; D010957:Plasmids; D012316:RNA Nucleotidyltransferases / Q000235:genetics*; D012328:RNA Viruses / Q000201:enzymology / Q000235:genetics*; D012367:RNA, Viral / Q000096:biosynthesis*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*; D014158:Transcription, Genetic
D004279:DNA, Viral; C028696:Qbeta ribonucleic acid; D012367:RNA, Viral; D012316:RNA Nucleotidyltransferases; D012324:RNA-Dependent RNA Polymerase