Assay-specific genotoxicity of N-nitrosodibenzylamine to the rat liver in vivo.
N-Nitrosodibenzylamine (NDBzA) is mutagenic to Salmonella typhimurium and induces DNA strand breaks in isolated rat hepatocytes, yet it is reported to be non-carcinogenic to the rat. Here we report that it is inactive in both the rat and mouse bone marrow micronucleus assays and in a rat liver autoradiographic assay for unscheduled DNA synthesis. It is, however, clearly active as a micronucleus-inducing agent and mitogen in the rat liver and is capable of inducing single-strand breaks in the DNA of rat liver. The origin and implications of this curious conflict of in vivo genotoxicity data are discussed. Irrespective of that discussion, it is concluded that NDBzA is genotoxic to the rat liver in vivo.
Institute of Toxicology and Chemotherapy, German Cancer Research Centre, Heidelberg, Federal Republic of Germany.
P
P
Schmezer
B L
BL
Pool
P A
PA
Lefevre
R D
RD
Callander
F
F
Ratpan
H
H
Tinwell
J
J
Ashby
D000818:Animals; D004247:DNA / Q000096:biosynthesis; D004249:DNA Damage; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015162:Micronucleus Tests; D015394:Molecular Structure; D009152:Mutagenicity Tests; D009602:Nitrosamines / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics
D009602:Nitrosamines; C015395:N-nitrosodibenzylamine; D004247:DNA