Characterization of human hematopoietic stem cells.
These data clearly indicate that suitable systems are presently available to isolate the human hematopoietic stem cell. The CFU-B1 and cells responsible for initiating long term hematopoiesis in vitro are likely identical since they share the same phenotype, CD34+DR-CD33-CD15-C71-. The suspension culture system as indicated above has several advantages over an adherent cell based long term marrow culture system. Use of this suspension culture system with 48 hourly feeding with recombinant cytokines will facilitate definition of the cytokine requirements for the human CFU-S.
Department of Medicine, Indiana University School of Medicine, Indianapolis 46202.
J E
JE
Brandt
E F
EF
Srour
K
K
van Besien
R
R
Hoffman
D000954:Antigens, Surface / Q000032:analysis; D001685:Biological Factors / Q000494:pharmacology; D001854:Bone Marrow Cells; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D046508:Culture Techniques / Q000379:methods; D016207:Cytokines; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D006801:Humans; D011994:Recombinant Proteins / Q000494:pharmacology
D000954:Antigens, Surface; D001685:Biological Factors; D016207:Cytokines; D011994:Recombinant Proteins