The progesterone receptor can regulate transcription in the absence of a functional TATA box element.
We have investigated the importance of the TATA box element in the induction of transcription by the progesterone receptor. Transcription was analyzed from promoters containing a steroid response element upstream of a wild-type or mutated TATA box. Mutation of the TATA box resulted in a loss of correctly initiated transcripts and abolished binding of TATA factor to the TATA box in vitro but did not inhibit transcriptional activation by the progesterone receptor. Thus we conclude that the receptor is able to stimulate the rate of transcription in the absence of a functional TATA box.
Molecular Endocrinology Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields, London, United Kingdom.
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Thomson
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Ham
O
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Bakker
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Parker
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D009154:Mutation; D011397:Promegestone / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D011980:Receptors, Progesterone / Q000187:drug effects / Q000378:metabolism*; D015183:Restriction Mapping; D016385:TATA Box; D013937:Thymidine Kinase / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection
D011980:Receptors, Progesterone; D011397:Promegestone; D013937:Thymidine Kinase