[Cyclosporin biosynthesis and dynamics of bioenergetic processes in cyclosporin-producing strain].
An attempt was made to show a correlation between definite bioenergetic parameters of the cells of the cyclosporine-producing culture and biosynthesis of cyclosporine. It was found that the three strains producing cyclosporine used in the study had an alternative cyanide-resistant pathway along with the classical cytochrome chain. In the strain forming only traces of the cyclopeptide during fermentation of the cyanide-resistant respiration constituted 60 to 80%. In the isogenic highly productive strains the cyanide-resistant respiration appeared to be markedly decreased beginning from the 1st day of fermentation and during the maximum biosynthesis of cyclosporine (on day 4 or 5 of fermentation) it reached zero. The ATP content in the cells of the highly productive strain, despite its decrease by the antibiotic biosynthesis peak, remained at a much higher level than that in the strain producing only traces of cyclosporine. A procedure for isolating functionally active mitochondria from the protoplasts was developed and a bioenergetic characterization of the mitochondria isolated from the strains with different antibiotic productions is presented.
Sotnikova
I V
IV
Telesnina
G N
GN
Zviagil'skaia
R A
RA
Ivanitskaia
L P
LP
Bibikova
M V
MV
Rybakova
A M
AM
Sazykin
Iu O
IuO
Navashin
S M
SM
D000255:Adenosine Triphosphate / Q000032:analysis; D003470:Culture Media; D003524:Cyclosporins / Q000096:biosynthesis*; D004734:Energy Metabolism; D008828:Microbiological Techniques; D008928:Mitochondria / Q000378:metabolism; D003904:Mitosporic Fungi / Q000378:metabolism*; D011523:Protoplasts / Q000378:metabolism; D013997:Time Factors
D003470:Culture Media; D003524:Cyclosporins; D000255:Adenosine Triphosphate
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