Intrinsic pituitary interleukin-1 beta is induced by bacterial lipopolysaccharide.
Using a specific antiserum recognizing recombinant rat interleukin-1 beta (IL-1 beta), immunoreactive material was localized to cytoplasmic granules in anterior pituitary endocrine cells and colocalized with TSH in thyrotropes. Authenticity was established by Northern blot hybridization using a specific rat IL-1 beta cRNA probe, revealing a 1.8-kilobase mRNA identical to that in the spleen. The marked increase in anterior pituitary IL-1 beta message after the administration of bacterial lipopolysaccharide, raises the possibility that IL-1 beta may be involved in paracrine or autocrine regulation of pituitary function during infectious challenge.
Department of Medicine, New England Medical Center Hospital, Boston, Massachusetts 02111.
J I
JI
Koenig
K
K
Snow
B D
BD
Clark
R
R
Toni
J G
JG
Cannon
A R
AR
Shaw
C A
CA
Dinarello
S
S
Reichlin
S L
SL
Lee
R M
RM
Lechan
D000818:Animals; D003593:Cytoplasm / Q000032:analysis; D004926:Escherichia coli; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D009693:Nucleic Acid Hybridization; D010903:Pituitary Gland, Anterior / Q000032:analysis / Q000378:metabolism* / Q000648:ultrastructure; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
D007375:Interleukin-1; D008070:Lipopolysaccharides; D015347:RNA Probes; D012333:RNA, Messenger