The ATP-dependent post translational modification of ferredoxin: NADP+ oxidoreductase.
Incubation of thylakoids with purified FNR and [32P]ATP led to the incorporation of phosphate into the FNR. In the absence of added FNR, 32P-labelled FNR could be detected associated with the thylakoids. An amino-acid analysis showed that in the dark, the FNR could be phosphorylated on a serine residue. In the presence of thylakoids, the FNR contained a threonine phosphate which was associated with a light-dependent reaction. The physiological function of this phosphorylation is not clear. Some modifications in NADP(+)-dependent photosystem I (PSI) activity and FNR-membrane association have been observed on the addition of ATP. Whether these changes are linked to the phosphorylation of the FNR remain to be fully elucidated.
Laboratoire de Physiologie Végétale Moléculaire, CNRS (UA1128), Université de Paris Sud, Orsay, France.
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Hodges
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Miginiac-Maslow
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Le Maréchal
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Rémy
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D002734:Chlorophyll / Q000235:genetics / Q000378:metabolism*; D002736:Chloroplasts / Q000201:enzymology*; D007887:Fabaceae / Q000378:metabolism*; D005287:Ferredoxin-NADP Reductase / Q000235:genetics / Q000378:metabolism*; D008027:Light; D045342:Light-Harvesting Protein Complexes; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D010084:Oxidation-Reduction; D010766:Phosphorylation; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D010946:Plants, Medicinal; D011499:Protein Processing, Post-Translational
D045342:Light-Harvesting Protein Complexes; D008565:Membrane Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D010940:Plant Proteins; C042085:thylakoid membrane phosphoprotein, plant; D002734:Chlorophyll; D000255:Adenosine Triphosphate; D005287:Ferredoxin-NADP Reductase; D009247:NADH, NADPH Oxidoreductases