Chemical characterization of lipopolysaccharides from Proteus strains used in Weil-Felix test.
The lipopolysaccharides (LPS) extracted from Proteus strains OX2, OX19, and OXK used as antigens in the Weil-Felix test, were characterized by chemical analysis and SDS-polyacrylamide gel electrophoresis (SDS-PAGE). To separate the O-polysaccharide, core-oligosaccharide, and lipid A moieties, each LPS was treated with 2% acetic acid, centrifuged, and applied to Sephadex G-50 column. The core-oligosaccharides contained L-glycero-D-mannoheptose, D-glycero-D-mannoheptose, glucose (Glc), galactose, 3-deoxy-D-mannooctulosonic acid, uronic acid, phosphate, glucosamine (GlcN), and galactosamine (GalN). The lipid A preparations contained GlcN, GlcN-phosphate, and three fatty acids (myristic, plamitic, and beta-hydroxymyristic acids). However, the O-polysaccharides of OX2- and OXK-LPS had different chemical compositions which consisted of Glc, GlcN, and quinovosamine, and Glc, uronic acid, and GalN, respectively, while OX19-LPS seemed to lack O-polysaccharide.
Mizushiri
S
S
Department of Bacteriology, Hirosaki University School of Medicine.
Amano
K
K
Fuji
S
S
Fukushi
K
K
Watanabe
M
M
D001431:Bacteriological Techniques; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000032:analysis; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000032:analysis; D011511:Proteus / Q000032:analysis*; D011513:Proteus mirabilis / Q000032:analysis; D011514:Proteus vulgaris / Q000032:analysis
D005227:Fatty Acids; D008070:Lipopolysaccharides