Demonstration of kinin-release in the peritoneal exudate of kaolin-induced writhing in mice.
Using a bradykinin enzyme immunoassay, we measured the amount of kinin in the peritoneal washings of mice with the kaolin-induced writhing reaction. Simultaneous treatment with captopril, a kininase II inhibitor, significantly increased the kinin level at 1 min after kaolin injection. Soybean trypsin inhibitor injected simultaneously with kaolin almost completely suppressed the kinin level at 1 min with or without treatment of captopril. These results suggest that kinin is released through activation of the plasma kallikrein-kinin system by kaolin, and that kinin could be a main mediator for the writhing reaction.
Department of Pharmacology, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
T
T
Fujiyoshi
M
M
Dozen
H
H
Iida
K
K
Ikeda
I
I
Hayashi
S
S
Oh-ishi
D000818:Animals; D002216:Captopril / Q000494:pharmacology; D005122:Exudates and Transudates / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D007274:Injections, Intraperitoneal; D007616:Kaolin / Q000008:administration & dosage; D007705:Kinins / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D014361:Trypsin Inhibitors / Q000494:pharmacology
D007705:Kinins; D014361:Trypsin Inhibitors; D007616:Kaolin; D002216:Captopril