Utilization of beet molasses for sterol production by some moulds.
Different moulds were cultivated in beet molasses (BM)-containing medium. Penicillium crustosum Thom was superior to the other moulds in total sterols production (4% on dry weight basis), efficiency of convertibility of the BM sugars to sterols (2%), total lipids (19.4%) and unsaponified lipids (13.4%). The treatment of BM with H2SO4- followed by centrifugation allowed maximum fermentation yields. The highest unsaponified lipids (16.5%) and total sterols level (7.4%) were obtained with a medium composed of (g/1): NaNO3, 3; K2HPO4, 3; MgSO4.7H2O, 1.5; K2SO4, 0.11; ZnSO4.7H2O, 0.05; FeCl3.6H2O, 0.16; H2SO4-treated BM, 60. Maximal sterol yields (8.4%) and high growth rate were achieved at the accelerated growth phase (8 days old cultures), when the initial pH value of the medium was adjusted to 7.0.
Ghanem
K M
KM
Botany Department, Faculty of Science, Alex. Uni., Egypt.
Ghanem
N B
NB
el-Refai
A H
AH
D050260:Carbohydrate Metabolism; D003470:Culture Media; D005285:Fermentation; D005658:Fungi / Q000254:growth & development / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000096:biosynthesis; D008670:Metals / Q000032:analysis; D008828:Microbiological Techniques; D008965:Molasses; D010407:Penicillium / Q000254:growth & development / Q000378:metabolism; D013261:Sterols / Q000096:biosynthesis*
D003470:Culture Media; D008055:Lipids; D008670:Metals; D013261:Sterols