Modeling of protease I collagenolytic enzyme from the fiddler crab Uca pugilator.
Collagenolytic protease I from the fiddler crab Uca pugilator belongs to the serine proteases of the trypsin family. A graphic molecular model was built using information from sequences and X-ray structures of four homologous proteins which were superimposed to define structurally conserved regions. Protease I sequence was aligned, with sequences of the model proteins, without permitting any deletion or insertion in these regions. Elastase alpha-carbon chain was selected as a template molecule. For the structurally variable regions, fragments of the four homologous proteins which were 'closet' in sequence were selected. Intramolecular steric hindrance, that resulted from the substitution of the residues of the templates by protease I residues, was corrected by adjustment of the side-chain conformational angles. The model was then optimized by energy minimization. The primary specificity pocket in the model of collagenolytic protease I predict a substrate preference for both P1 hydrophobic and positively charged residues which is in agreement with the biochemical observations. As soybean trypsin inhibitor (STI) is known to inhibit collagenolytic protease I, a tentative model of the complex was constructed and possibilities of interaction examined.
Institut de Chimie des Substances Naturelles, C.N.R.S., Gif-sur-Yvette, France.
B
B
Arnoux
A
A
Lecroisey
A
A
Ducruix
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D003386:Brachyura / Q000201:enzymology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012689:Sequence Homology, Nucleic Acid; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure*; D015842:Serine Proteinase Inhibitors; D012984:Software; D014361:Trypsin Inhibitors / Q000378:metabolism
D015842:Serine Proteinase Inhibitors; D014361:Trypsin Inhibitors; D012697:Serine Endopeptidases; C066053:collagenolytic protease I, crab