A macrophage LPS-inducible early gene encodes the murine homologue of IP-10.
Recently, we have isolated and characterized a set of cDNA clones which encode lipopolysaccharide-inducible proteins in murine peritoneal macrophages. Here, we report the sequence and identification of one of these cDNAs previously termed C7. Nucleotide sequence analysis revealed an open reading frame encoding a predicted polypeptide composed of 98 amino acids, which contained a 21 amino acid residue signal peptide, indicating approximately 9 kDa of mature protein. The deduced protein sequence showed homology (67% identity, 77% considering conservative amino acid changes) with the human INF gamma-inducible gene IP-10, a member of the recently described superfamily of chemotactic and mitogenic proteins which includes platelet factor 4, monocyte-derived neutrophil chemotactic factor (NAF, NAP-1, IL-8), and MGSA/gro/KC. Thus C7 would appear to represent the murine homologue of the human IP-10 gene or a very closely related gene.
Ohmori
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Research Institute, Cleveland Clinic Foundation, Ohio 44195.
Hamilton
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TA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001685:Biological Factors / Q000235:genetics*; D054357:Chemokine CXCL10; D019743:Chemokines, CXC; D003001:Cloning, Molecular; D016207:Cytokines; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D010529:Peritoneal Cavity / Q000166:cytology; D011506:Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D001685:Biological Factors; D054357:Chemokine CXCL10; D019743:Chemokines, CXC; D016207:Cytokines; D008070:Lipopolysaccharides; D011506:Proteins