An extracellular microelectrode array for monitoring electrogenic cells in culture.
This paper describes a planar array of microelectrodes developed for monitoring the electrical activity of cells in culture. The device allows the incorporation of surface topographical features in an insulating layer above the electrodes. Semiconductor technology is employed for the fabrication of the gold electrodes and for the deposition and patterning of an insulating layer of silicon nitride. The electrodes have been tested using a cardiac cell culture of chick embryo myocytes, and the physical beating of the cultured cells correlated with the simultaneous extracellular voltage measurements obtained. It was found that extracellular stimulation of the cells was possible via the same electrodes used for recording.
Connolly
P
P
Department of Electronics and Electrical Engineering, University of Glasgow, UK.
Clark
P
P
Curtis
A S
AS
Dow
J A
JA
Wilkinson
C D
CD
D000200:Action Potentials; D000818:Animals; D015374:Biosensing Techniques; D002478:Cells, Cultured; D004558:Electric Stimulation; D004563:Electrochemistry; D005110:Extracellular Space / Q000378:metabolism*; D008839:Microelectrodes; D009200:Myocardial Contraction / Q000502:physiology; D009206:Myocardium / Q000166:cytology / Q000378:metabolism; D012666:Semiconductors