A comparison of HEp-2 cell invasion by enteropathogenic and enteroinvasive Escherichia coli.
In order to further characterize cellular invasion by enteropathogenic Escherichia coli (EPEC), we compared invasion of HEp-2 cells by EPEC and enteroinvasive E. coli (EIEC). We used a gentamicin HEp-2 cell assay and measured bacterial recovery under conditions of varying incubation time and temperature, and in the presence or absence of inhibitors of cellular microfilaments and microtubules. We found that, unlike EIEC, EPEC did not rapidly multiply within HEp-2 cell but invaded well at 32 degrees C. While microfilament inhibitors reduced invasion by both EIEC and EPEC, microtubule inhibitors reduced invasion by EPEC only. These results suggest that EPEC and EIEC differ in their mechanisms of epithelial cell invasion.
Division of Geographic Medicine and Infectious Diseases, New England Medical Center, Boston, MA.
M S
MS
Donnenberg
A
A
Donohue-Rolfe
G T
GT
Keusch
D008841:Actin Cytoskeleton / Q000187:drug effects / Q000502:physiology; D000199:Actins / Q000502:physiology; D000818:Animals; D002460:Cell Line; D003571:Cytochalasin B / Q000494:pharmacology; D015638:Cytochalasin D / Q000494:pharmacology; D004848:Epithelium / Q000382:microbiology*; D004926:Escherichia coli / Q000254:growth & development / Q000472:pathogenicity* / Q000502:physiology; D005839:Gentamicins / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors
D000199:Actins; D005839:Gentamicins; D015638:Cytochalasin D; D003571:Cytochalasin B