Endothelin does not affect intracellular calcium ion concentration in the platelets of rats.
We recently demonstrated that porcine endothelin increases intracellular calcium ion concentration ([Ca2+]i) in monolayers of cultured vascular smooth muscle cells isolated from rat renal arteries. The present study was designed to examine the effect of porcine endothelin on [Ca2+]i in the platelets of rats. [Ca2+]i was serially measured with the fluorescent dye, fura 2, and the photoprotein, aequorin. Endothelin (10(-9) mol/L, 10(-8) mol/L, and 10(-7) mol/L) did not induce any change in [Ca2+]i in the platelets of rats while thrombin (2 U/mL) dramatically increased [Ca2+]i as measured by these methods. Our results indicate that [Ca2+]i in rat platelets is not influenced by porcine endothelin.
First Department of Internal Medicine, Osaka City University Medical School, Japan.
K
K
Murakawa
M
M
Kohno
K
K
Yokokawa
K
K
Yasunari
N
N
Kurihara
A
A
Hyono
T
T
Takeda
D000331:Aequorin; D000818:Animals; D001572:Benzofurans; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D002118:Calcium / Q000097:blood*; D016232:Endothelins; D004730:Endothelium, Vascular; D005456:Fluorescent Dyes; D016257:Fura-2; D008297:Male; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence; D013552:Swine; D013917:Thrombin / Q000378:metabolism
D001572:Benzofurans; D016232:Endothelins; D005456:Fluorescent Dyes; D010455:Peptides; D000331:Aequorin; D013917:Thrombin; D002118:Calcium; D016257:Fura-2