The role of EF-Tu and other translation components in determining translocation step size.
The two EF-Tu encoding genes, tufA and tufB, of Salmonella typhimurium have been sequenced. Nearly all the differences from their Escherichia coli counterparts are third position changes which do not alter the encoded amino acids. Unexpectedly, most of the changes in one Salmonella tuf gene are paralleled by changes in the other tuf gene perhaps due to gene repair despite the distance separating the genes. Three mutants which cause mis-framing, have their substitutions at codon 375. Explanations for mutants which cause mis-framing are considered and the mechanism of normal reading frame maintenance discussed.
Howard Hughes Medical Institute, University of Utah, Salt Lake City 84132.
T M
TM
Tuohy
S
S
Thompson
R F
RF
Gesteland
D
D
Hughes
J F
JF
Atkins
D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D015345:Oligonucleotide Probes; D010444:Peptide Elongation Factor Tu / Q000235:genetics* / Q000378:metabolism; D014176:Protein Biosynthesis; D012486:Salmonella typhimurium / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D015345:Oligonucleotide Probes; D010444:Peptide Elongation Factor Tu