Artificial reversion of acute myeloid leukemia cells into normal phenotype.
1. Induction of tumor cell differentiation could reverse transformed cells into normal, mature cells. Important question is whether these malignant-to-normal reversed cells are really normal ones. 2. We have developed an experimental model based on the examination of three different levels of human acute myeloid leukemia cell properties before and after induction of differentiation: morphological (percentage of undifferentiated blast cells), functional (DNA ploidy, Fc receptors, phagocytic activity, clonogenic assay in soft agar, oxidative metabolism which accompanies phagocytosis in mature granulocytes) and genetical (expression of oncogene p53). 3. Several inducers have been employed: dimethylsulfoxide (DMSO) granulocyte-macrophage colony stimulating factor (GM-CSF); tunicamycin, interferon gamma, tumor necrosis factor and lipopolysaccharide. 4. Our results indicate that the reversion of leukemic cells into mature normal ones with some inducers (DMSO, GM-CSF) could be a complete process.
Pavelić
K
K
Rugjer Bosković Institute, Zagreb, Yugoslavia.
Baltić
V
V
Spaventi
S
S
D002454:Cell Differentiation; D002471:Cell Transformation, Neoplastic; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D004247:DNA / Q000032:analysis; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D010587:Phagocytosis; D010641:Phenotype; D011003:Ploidies; D011961:Receptors, Fc / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014415:Tunicamycin / Q000494:pharmacology
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007375:Interleukin-1; D008070:Lipopolysaccharides; D011961:Receptors, Fc; D014409:Tumor Necrosis Factor-alpha; D014415:Tunicamycin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D004247:DNA; D004121:Dimethyl Sulfoxide