Genetic abnormalities in sporadic parathyroid adenomas.
We analyzed genomic DNA from 43 sporadic benign parathyroid adenomas for rearrangements of the PTH gene, and for point mutations of the H-ras (codons 12, 13, and 61), N-ras (codons 12, 13, and 61), and K-ras (codons 12 and 13) genes. One of 43 parathyroid adenomas showed a chromosome 11 rearrangement involving both the PTH gene on the short arm of chromosome 11 (at band p15) and a locus on the long arm (11q13). This rearrangement was indistinguishable from one that was previously described in a parathyroid adenoma by Arnold et al., indicating that this may be an important contributor to tumorigenesis in a small subset of patients with parathyroid adenoma. H-ras, K-ras, and N-ras oncogene activation by point mutation at codons 12, 13, or 61, known to occur in many tumors, could not be detected in any parathyroid adenoma.
Molecular Pathophysiology Branch, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
E
E
Friedman
A E
AE
Bale
S J
SJ
Marx
J A
JA
Norton
A
A
Arnold
T
T
Tu
G D
GD
Aurbach
A M
AM
Spiegel
D000236:Adenoma / Q000175:diagnosis / Q000235:genetics*; D015139:Blotting, Southern; D002880:Chromosomes, Human, Pair 11; D003062:Codon / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015321:Gene Rearrangement; D011905:Genes, ras; D006801:Humans; D006961:Hyperparathyroidism / Q000209:etiology; D009154:Mutation; D010281:Parathyroid Hormone / Q000235:genetics*; D010282:Parathyroid Neoplasms / Q000175:diagnosis / Q000235:genetics*
D003062:Codon; D010281:Parathyroid Hormone; D004247:DNA