[Effect of hexamethylene bisacetamide on human gastric cancer cell line SGC-7901].
After the human gastric cancer cells SGC-7901 were cultured with 5 mM hexamethylene bisacetamide (HMBA) for 120 hr, cell growth was inhibited. The doubling time increased from 48 hr in untreated cells to 99.6 hr in HMBA-treated cells. The maximal increase in growth was 2.4-fold as compared to 6.4-fold in the untreated control. 3H-thymidine incorporation was inhibited by HMBA and the colony formation rate was reduced. There was concomitant decrease in carcino-embryonic antigen and beta-2 microglobulin as determined by radioimmuno-assay. Lactic dehydrogenase (LDH) and its isoenzyme assay revealed a marked increase in H-type LDH but the total enzyme activity was reduced. These results indicate that HMBA inhibits proliferation of SGC-7901 cells in vitro.
Second Hospital, Lanzhou Medical College.
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D000081:Acetamides / Q000494:pharmacology*; D002272:Carcinoembryonic Antigen / Q000032:analysis; D002455:Cell Division / Q000187:drug effects; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D013274:Stomach Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D001613:beta 2-Microglobulin / Q000378:metabolism
D000081:Acetamides; D002272:Carcinoembryonic Antigen; D004273:DNA, Neoplasm; D007527:Isoenzymes; D001613:beta 2-Microglobulin; D007770:L-Lactate Dehydrogenase; C014026:hexamethylene bisacetamide