Prevention of Escherichia coli K1 bacteremia in newborn mice by using topical vaginal carbohydrates.
Pregnant Swiss-Webster mice were vaginally inoculated with 5 x 10(4) Escherichia coli K1 strain LH (O75:K1:H3) or C94 (O7:K1:H-). Inhibitor solutions were applied vaginally before delivery and the incidence of bacteremia and surface colonization determined in neonates at 3 days of age. E. coli K1 strain LH resulted in bacteremia in 77% and colonization in 74% of control newborn mice. After topical maternal vaginal D-mannose treatment, bacteremia and colonization were present in 25% of neonates. Topical vaginal application of subinhibitory concentration of gentamicin reduced bacteremia to 23% of neonates. Topical methyl-alpha-D-mannoside and p-nitrophenyl-D-mannoside, however, prevented bacteremia in 100% of newborn mice. A neonatal meningitis strain of E. coli K1 (C94) caused bacteremia in 100% of neonates and was also completely inhibited by methyl-alpha-D-mannoside. This technique of vaginal treatment before delivery may have applicability to human mothers and their infants.
Department of Pediatrics, Medical College of Georgia, Augusta 30912.
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D000282:Administration, Intravaginal; D000818:Animals; D000831:Animals, Newborn; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000517:prevention & control* / Q000635:transmission; D005260:Female; D005839:Gentamicins / Q000627:therapeutic use; D006027:Glycosides / Q000627:therapeutic use*; D008358:Mannose / Q000008:administration & dosage / Q000627:therapeutic use*; D008362:Mannosides / Q000008:administration & dosage / Q000627:therapeutic use*; D051379:Mice; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000188:drug therapy; D018805:Sepsis / Q000517:prevention & control*
D005839:Gentamicins; D006027:Glycosides; D008362:Mannosides; D008358:Mannose