The use of biotin-labelled, synthetic DNA oligomers for the detection and identification of Plasmodium falciparum.
An oligonucleotide mixture based on the 21 base pair repeat sequence of Plasmodium falciparum was covalently coupled to biotin and used as a probe to detect P. falciparum DNA. The limit of detection was 10 ng. This method was further developed as a fingerprint assay for parasite strain typing. After restriction enzyme digestion, blotting and hybridization, distinct banding patterns were obtained for the strains tested and these were reproducible. In addition, discrete differences were found between PLF-3 S+/S-strains which may implicate genetic reorganization in the switching mechanism which occurs when parasites are passed from an intact to a splenectomized animal.
Wolfson Unit of Molecular Genetics, Liverpool School of Tropical Medicine.
M A
MA
Hughes
M
M
Hommel
J M
JM
Crampton
D000818:Animals; D001483:Base Sequence; D001710:Biotin; D004247:DNA / Q000032:analysis*; D015151:Immunoblotting; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009710:Nucleotide Mapping; D015345:Oligonucleotide Probes; D010963:Plasmodium falciparum / Q000235:genetics / Q000302:isolation & purification*; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity
D015345:Oligonucleotide Probes; D001710:Biotin; D004247:DNA