Isolation, purification and characterization of surface antigens of the bovine filarial parasite Setaria digitata for the immunodiagnosis of bancroftian filariasis.
The surface antigens of the bovine filarial parasite Setaria digitata were isolated by EDTA extraction and purified by affinity chromatography using sepharose bound human filarial (Wuchereria bancrofti) antibodies obtained from chronic human filarial sera. The purified and crude antigens were used in enzyme-linked immunosorbent assay (ELISA) for the detection of serum antibodies in bancroftian filariasis. The purified antigen showed sensitive and specific reactions in ELISA for the detection of antibodies in filarial sera and showed least cross reactivity with other parasitic infections. The crude and purified antigens showed about 18 and 6 peptide bands respectively in SDS-PAGE and about 11 and 6 antigenic bands respectively in enzyme-linked immunoelectrotransfer blot (EITB). The purified antigen was observed to be glycoprotein in nature. It was possible to identify the stage-specific infection in human filariasis by using the crude and purified antigens in EITB.
Theodore
J G
JG
Center for Biotechnology, Anna University, Madras, India.
Kaliraj
P
P
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D000947:Antigens, Helminth / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification; D002846:Chromatography, Affinity; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004605:Elephantiasis, Filarial / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D005368:Filariasis / Q000175:diagnosis*; D005370:Filarioidea / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D012719:Setariasis / Q000469:parasitology; D014957:Wuchereria / Q000276:immunology*; D014958:Wuchereria bancrofti / Q000276:immunology*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000954:Antigens, Surface