Construction of a new catabolic pathway for D-fructose in Escherichia coli K12 using an L-sorbose-specific enzyme from Klebsiella pneumoniae.
Starting with a fruK (formerly fpk) mutant of Escherichia coli K12 lacking D-fructose-1-phosphate kinase (E.C. 2.7.1.3.), fructose positive derivatives were isolated after introduction of the cloned gene sorE from Klebsiella pneumoniae coding for an L-sorbose-1-phosphate reductase. The new pathway was shwon to proceed from D-fructose via D-fructose-1-phosphate and D-mannitol-1-phosphate to D-fructose 6-phosphate. It involves a transport system and enzymes encoded in the fru and the mtl operons from E. coli K12 as well as in the sor operon from K. pneumoniae respectively.
Wöhrl
B M
BM
Max-Planck-Institut für Molekulare Genetik, Berlin, Federal Republic of Germany.
Sprenger
G A
GA
Lengeler
J W
JW
D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005632:Fructose / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics; D009154:Mutation; D009876:Operon; D011815:R Factors; D015183:Restriction Mapping; D013013:Sorbose / Q000378:metabolism; D013401:Sugar Alcohol Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D005632:Fructose; D013401:Sugar Alcohol Dehydrogenases; C036132:L-sorbose-1-phosphate reductase; D013013:Sorbose