Regeneration of NADH and ketone hydrogenation by hydrogen with the combination of hydrogenase and alcohol dehydrogenase. Scientific note.
The regeneration of nicotinamide-adenine dinucleotide (reduced form, NADH) by the reaction of NAD with hydrogen gas was carried out in the presence of the hydrogenase from Alcaligenes eutrophus. And the formations of alcohol, CO2, and 6-phospho-gluconate were observed by a combination of the above system and corresponding dehydrogenases. NADH was regenerated by hydrogen gas with the hydrogenase and recycled in these reactions.
Okura
I
I
Department of Bioengineering, Tokyo Institute of Technology, Japan.
Otsuka
K
K
Nakada
N
N
Hasumi
F
F
D000421:Alcaligenes / Q000201:enzymology; D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D000438:Alcohols / Q000378:metabolism; D006859:Hydrogen; D006864:Hydrogenase / Q000378:metabolism*; D007659:Ketones / Q000378:metabolism*; D007700:Kinetics; D009243:NAD / Q000378:metabolism*; D010084:Oxidation-Reduction
D000438:Alcohols; D007659:Ketones; D009243:NAD; D006859:Hydrogen; D000426:Alcohol Dehydrogenase; D006864:Hydrogenase