Isolation of a novel protein kinase-encoding gene from yeast by oligodeoxyribonucleotide probing.
We have identified a novel protein kinase-encoding gene, KIN3, in the genome of the budding yeast Saccharomyces cerevisiae. The gene was isolated from a library of cloned genomic fragments by probing with an oligodeoxyribonucleotide mixture corresponding to part of a highly-conserved region in the catalytic domain of protein serine-threonine kinases. KIN3 is unique in the yeast genome, maps to chromosome VI and is actively expressed in mitotically dividing cells to produce a 1400 nucleotide (nt) message. The nt sequence of KIN3 predicts a protein product of 43.4 kDa which contains all of the conserved elements found in known protein serine-threonine kinases, although the organisation of these elements in the KIN3 gene product differs significantly from the consensus. The function of the KIN3-encoded protein kinase is unclear although it appears not to be essential for growth, conjugation or sporulation.
Department of Biochemistry and Molecular Biology, University of Manchester, U.K.
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Jones
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Rosamond
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015345:Oligonucleotide Probes; D011494:Protein Kinases / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
D005656:Fungal Proteins; D015345:Oligonucleotide Probes; D029701:Saccharomyces cerevisiae Proteins; D011494:Protein Kinases; C075981:KIN3 protein, S cerevisiae; D017346:Protein Serine-Threonine Kinases