Solution synthesis of human peptide YY (hPYY).
Human peptide YY (hPYY) was synthesized in a conventional manner by assembling six peptide fragments followed by deprotection with 1 M trimethylsilyl trifluoromethanesulfonate (TMSOTf)-thioanisole in trifluoroacetic acid (TFA). After purification by gel-filtration on Sephadex G-25, followed by reversed-phase high-performance liquid chromatography, a highly purified sample of synthetic hPYY was obtained. When administered in dogs, synthetic hPYY was as active as synthetic porcine PYY in terms of the effects on systemic arterial blood pressure, and splanchnic blood flow.
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
K
K
Kitagawa
T
T
Tomoyasu
S
S
Futaki
T
T
Akita
S
S
Higashide
K
K
Inoue
T
T
Tobe
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008969:Molecular Sequence Data; D019894:Peptide YY; D010455:Peptides / Q000138:chemical synthesis*
D010455:Peptides; D019894:Peptide YY