Immunology of atherosclerosis: cellular composition and major histocompatibility complex class II antigen expression in aortic intima, fatty streaks, and atherosclerotic plaques in young and aged human specimens.
There is evidence that fatty streaks in arteries can transform into atherosclerotic plaques. Mononuclear cells, including both monocytes and lymphocytes, are among the first cells participating in the development of atherosclerosis of experimental animals. To investigate the roles of different cell types in human atherosclerosis, we enumerated and compared the cellular compositions of normal intima, the transition zone (the area between the normal intima and the core of fatty streaks), fatty streaks, and plaques in young (age 16-30 years) and aged (over 60 years) human specimens using double-staining immunofluorescence with a series of monoclonal and polyclonal antibodies. T lymphocytes, both T helper/inducer (70% of T cells) and T suppressor/cytotoxic (30%) phenotypes, were found in every stage of atherosclerosis, constituting 30 to 40% of total cells in fatty streaks and transition zones of young subjects, and occasionally even in normal intima. Seventy percent of these T cells were HLA-DR positive, which indicated that most of them were activated. Macrophages were most frequent in fatty streaks and around the necrotic core of plaques. Smooth muscle cells, increasing from 5 to 30% with lesion progression, were HLA-DR positive where activated T helper cells occurred in the vicinity. The intracellular presence of the invariant gamma chain confirmed that HLA-DR was actually synthesized by these smooth muscle cells. Endothelial cells were HLA-DR positive above those regions of the lesions where HLA-DR-positive cells had accumulated, but not in normal intima, again suggesting induction of HLA-DR expression by T-cell-derived gamma-interferon. Furthermore, most HLA-DR-positive cells were also identified as HLA-DP and HLA-DQ positive. This aberrant major histocompatibility complex class II antigen expression in smooth muscle and endothelial cells may participate in the perpetuation of the atherogenetic autoimmune reaction.
Institute for General and Experimental Pathology, University of Innsbruck Medical School, Austria.
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Xu
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Oberhuber
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Gruschwitz
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Wick
D000293:Adolescent; D000328:Adult; D000375:Aging / Q000276:immunology*; D000943:Antigens, Differentiation / Q000032:analysis; D001011:Aorta / Q000166:cytology / Q000276:immunology*; D001161:Arteriosclerosis / Q000276:immunology* / Q000473:pathology*; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D016167:Integrin alphaXbeta2; D008264:Macrophages / Q000276:immunology; D008297:Male; D008875:Middle Aged; D016028:Receptors, Leukocyte-Adhesion / Q000032:analysis
D000943:Antigens, Differentiation; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D016167:Integrin alphaXbeta2; D016028:Receptors, Leukocyte-Adhesion