Silver accumulation and resistance in Escherichia coli R1.
E. coli R1 contains at least 2 large plasmids (83 and 77 kb) while E. coli S1 was previously cured of the 83 kb plasmid. Transformation using artificial competence, high-voltage electroporation, and plasmid mobilization experiments with the mobilizing plasmid RP4, failed to ascertain the 83 kb plasmid was responsible for Ag(+)-resistance. Silver accumulation by an Ag(+)-sensitive E. coli S1 strain was 5-fold higher than an Ag(+)-resistant E. coli R1 strain. The Ag(+)-resistant E. coli R1 strain produced 33% more H2S and 32% more intracellular acid labile SH than the Ag(+)-sensitive E. coli R1 strain when grown in the absence of AgNO3. Hydrophobic interaction chromatography revealed E. coli R1 displayed higher cell surface hydrophobicity than E. coli S1. HPLC protein analysis of cell-free extracts also revealed differences between protein fractions in E. coli R1 and S1 strains.
Department of Environmental Biology, University of Guelph, Ontario, Canada.
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D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003227:Conjugation, Genetic / Q000502:physiology; D004352:Drug Resistance, Microbial / Q000502:physiology; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D006862:Hydrogen Sulfide; D011815:R Factors / Q000235:genetics; D012834:Silver / Q000378:metabolism* / Q000494:pharmacology; D014170:Transformation, Genetic / Q000502:physiology
D012834:Silver; D006862:Hydrogen Sulfide