Antisera to a synthetic peptide recognize native and denatured rat estrogen receptors.
Specific polyclonal antisera to the rat estrogen receptor (rER) were developed using a synthetic peptide as the antigen. The peptide corresponds to amino acids 270-284 deduced from the cloned rER cDNA and has no homology to other steroid hormone receptors. Antipeptide antisera raised in rabbits specifically recognize a 67,000 mol wt protein, shown to be the rER, in Western blot experiments. In immunoprecipitation experiments, one tested antiserum bound unoccupied as well as 17 beta-estradiol-occupied rERs, indicating that this region is exposed in both receptor forms. This antiserum shows no cross-reactivity with rat progesterone or glucocorticoid receptors. Cross-reactivity with rabbit, human, and, to a lesser extent, bovine ERs was observed.
Furlow
J D
JD
Department of Biochemistry, University of Wisconsin, Madison 53706.
Ahrens
H
H
Mueller
G C
GC
Gorski
J
J
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology*; D015153:Blotting, Western; D006433:Hemocyanins / Q000276:immunology; D007106:Immune Sera / Q000276:immunology*; D007163:Immunosorbent Techniques; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010047:Ovalbumin / Q000276:immunology; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology*; D011487:Protein Conformation; D011489:Protein Denaturation; D051381:Rats; D011919:Rats, Inbred Strains; D011960:Receptors, Estrogen / Q000276:immunology*
D000941:Antigens; D007106:Immune Sera; D010446:Peptide Fragments; D011960:Receptors, Estrogen; D010047:Ovalbumin; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin