Modulation of HLA-DR expression in epithelial cells by interleukin 1 and estradiol-17 beta.
Both ultrapure human interleukin-1 (IL-1) and Escherichia coli derived recombinant IL-1 alpha and beta consistently induced the expression of major histocompatibility class II (HLA-DR) molecules in a human endometrial and a breast carcinoma cell line. [35S]Methionine incorporation into IL-1 induced, immunoprecipitable HLA-DR molecules demonstrated de novo synthesis of both light and heavy chains of the HLA-DR molecules. Lipopolysaccharide, recombinant interleukin-2 and recombinant interleukin-6 failed to induce HLA-DR expression in these epithelial cells. In contrast to the dramatic effect on HLA-DR expression, IL-1 had no effect on the epithelial cell proliferation. Pretreatment of T47D cells with estradiol-17 beta significantly decreased the IL-1 induced HLA-DR expression, and pretreatment of IL-1 with an IL-1 specific antibody, neutralized IL-1 action. These studies demonstrate that a cytokine (IL-1) and a sex steroid hormone estradiol-17 beta can interact to regulate the expression of HLA-DR molecules in epithelial cells.
Department of Pathology, City Hospital Center, Elmhurst, New York 11373.
S S
SS
Tabibzadeh
A
A
Sivarajah
D
D
Carpenter
B M
BM
Ohlsson-Wilhelm
P G
PG
Satyaswaroop
D004848:Epithelium / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006684:HLA-DR Antigens / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000276:immunology / Q000494:pharmacology*; D008715:Methionine / Q000378:metabolism; D011233:Precipitin Tests; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D006684:HLA-DR Antigens; D007375:Interleukin-1; D011994:Recombinant Proteins; D004958:Estradiol; D008715:Methionine