Analysis of side-chain conformational distributions in neutrophil peptide-5 NMR structures.
The side-chain conformations have been analyzed in the antimicrobial peptide, Neutrophil Peptide-5 (NP-5), whose structure was independently generated from nmr-derived distance constraints using a distance geometry algorithm. The side-chain and peptide dihedral angle distributions in the nmr structures were compared with those constructed from a data base of high-resolution protein crystal structures. The side-chain conformational preferences for NP-5 in solution are significantly different from those observed in the crystal structure data base. These results indicate that the side-chain conformations are quite disordered for many of the residues of NP-5. The absence of a correlation between the width of the conformational distribution and surface accessibility suggests that the disorder may be due to limitations in the structural information extracted from the nmr data rather than to molecular motion. However, it is also observed that the degree of conformational disorder is only weakly correlated with the number of nuclear Overhauser enhancements to a given side chain. Possible reasons for this are discussed. Molecular mechanics refinement of these structures did not significantly change the side-chain populations. Anomolously wide distributions are observed for rotations about the peptide bonds and the disulfide bonds in the NP-5 distance geometry structures, which are improved by the refinement. The very high degree of order observed for the central dihedral angle of the disulfide bond in the high-resolution crystal data base suggests that the rotation about this bond in proteins is determined by the local potential.
Kominos
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D
Department of Chemistry, Rutgers University, New Brunswick, New Jersey 08903.
Bassolino
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DA
Levy
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RM
Pardi
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A
D000465:Algorithms; D000596:Amino Acids; D001798:Blood Proteins; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D023082:Defensins; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D009504:Neutrophils; D011487:Protein Conformation; D014961:X-Ray Diffraction; D023084:alpha-Defensins
D000596:Amino Acids; D001798:Blood Proteins; D023082:Defensins; D023084:alpha-Defensins; C406429:neutrophil peptide 5