Genetic abnormalities in sporadic parathyroid adenomas.
We analyzed genomic DNA from 43 sporadic benign parathyroid adenomas for rearrangements of the PTH gene, and for point mutations of the H-ras (codons 12, 13, and 61), N-ras (codons 12, 13, and 61), and K-ras (codons 12 and 13) genes. One of 43 parathyroid adenomas showed a chromosome 11 rearrangement involving both the PTH gene on the short arm of chromosome 11 (at band p15) and a locus on the long arm (11q13). This rearrangement was indistinguishable from one that was previously described in a parathyroid adenoma by Arnold et al., indicating that this may be an important contributor to tumorigenesis in a small subset of patients with parathyroid adenoma. H-ras, K-ras, and N-ras oncogene activation by point mutation at codons 12, 13, or 61, known to occur in many tumors, could not be detected in any parathyroid adenoma.
Friedman
E
E
Molecular Pathophysiology Branch, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Bale
A E
AE
Marx
S J
SJ
Norton
J A
JA
Arnold
A
A
Tu
T
T
Aurbach
G D
GD
Spiegel
A M
AM
D000236:Adenoma / Q000175:diagnosis / Q000235:genetics*; D015139:Blotting, Southern; D002880:Chromosomes, Human, Pair 11; D003062:Codon / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015321:Gene Rearrangement; D011905:Genes, ras; D006801:Humans; D006961:Hyperparathyroidism / Q000209:etiology; D009154:Mutation; D010281:Parathyroid Hormone / Q000235:genetics*; D010282:Parathyroid Neoplasms / Q000175:diagnosis / Q000235:genetics*
D003062:Codon; D010281:Parathyroid Hormone; D004247:DNA