Use of an alpha-D-glucosidase for the specific cytochemical identification of lateral alpha-D-xylosyl end groups in plant xyloglucans.
alpha-Linked D-xylosyl side chains represent the typical feature common to all xyloglucans not shared by other cell wall polysaccharides. Since no easily available alpha-D-xyloxidase is known, advantage was taken of the conformational and configurational homologies between alpha-D-xylopyranose and alpha-D-glucopyranose to make an alpha-D-glucosidase-gold complex which was able to recognize alpha-D-xylosyl terminal residues of xyloglucans. This marker was used together with alpha-L-fucosidase gold complex for the double labeling on two different structural features of the same macromolecule in plant primary cell wall.
Ruel
K
K
Centre de Recherches sur les Macromolécules Végétales, C.N.R.S., Grenoble, France.
Joseleau
J P
JP
D002473:Cell Wall / Q000032:analysis; D005936:Glucans; D006046:Gold; D006651:Histocytochemistry; D007202:Indicators and Reagents; D008854:Microscopy, Electron; D010944:Plants / Q000032:analysis* / Q000648:ultrastructure; D011134:Polysaccharides / Q000032:analysis*; D014990:Xylans; D014994:Xylose / Q000032:analysis*; D000520:alpha-Glucosidases; D005644:alpha-L-Fucosidase
D005936:Glucans; D007202:Indicators and Reagents; D011134:Polysaccharides; D014990:Xylans; C029353:xyloglucan; D006046:Gold; D014994:Xylose; D000520:alpha-Glucosidases; D005644:alpha-L-Fucosidase