Immunotherapy of collagen-induced arthritis by a T-cell antiproliferative molecule.
The present study describes a novel experimental immunotherapeutic methodology for the reduction of inflammatory synovitis that is noted in an animal model of rheumatoid arthritis. The reduction in inflammation is noted in the animals administered a contra-interleukin-2 (IL-2) cytokine secreted by a cloned T-cell line. The mechanism of reduction of inflammation by this cytokine is through the inhibition of activation and differentiation of T lymphocytes. The cytokine inhibits the in vitro mitogen activation of T-cell lymphocytes as well as antigen-specific activation of a collagen type II specific T-cell line. In addition, decreased levels of messenger RNA coding for interleukin-2 are noted in T lymphocytes and IL-2 activation of the collagen type II specific cell line is inhibited by the contra-IL-2 cytokine. This initial description of a reduction in inflammation by a contra-IL-2 lymphokine suggests that immunoregulatory biologic molecules that are antagonists to IL-2 may be useful for the experimental immunotherapy of cartilage connective tissue pathology.
Department of Medicine, Miriam Hospital, Providence, Rhode Island 02906.
D J
DJ
Spannaus-Martin
R
R
Holmdahl
T F
TF
Kresina
D000818:Animals; D001168:Arthritis / Q000188:drug therapy*; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D001685:Biological Factors / Q000494:pharmacology / Q000627:therapeutic use*; D001752:Blast Crisis / Q000473:pathology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003094:Collagen; D016207:Cytokines; D006825:Hybridomas / Q000378:metabolism / Q000473:pathology; D007376:Interleukin-2 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism; D013945:Thymoma / Q000378:metabolism / Q000473:pathology
D001685:Biological Factors; D016207:Cytokines; D007376:Interleukin-2; D012333:RNA, Messenger; D003094:Collagen