Detection of respiratory syncytial virus antigen after seventy-two hours of culture.
Between September, 1987, and April, 1989, three techniques for the detection of respiratory syncytial virus (RSV) were compared: indirect immunofluorescence (IF) on the sample, indirect immunofluorescence after 72 h of MRC-5 cell culture (IF 72h), and detection of the cytopathic effect (CPE) by MRC-5 and HEp-2 cell culture. A study of 383 nasal aspirates from young children admitted to the Centre Hospitalier de Nantes (CHR) showing miscellaneous respiratory symptoms produced the following results: 143 samples (37%) were RSV positive by IF, 119 (31%) were positive by IF 72h, and 117 (31%) showed RSV-induced CPE. In comparison with tissue culture isolation (TC), the sensitivities of IF and IF 72h were 89% and 80% and their specificities 85% and 91%, respectively. During the winter of 1988-1989, of the 110 RSV-positive nasal aspirates (104 by IF, 89 by IF 72h, 83 by CPE detection), 109 were identified by IF and/or IF 72h. IF 72h affords rapid detection of RSV.
Laboratory of Virology, Regional Hospital Centre, Nantes, France.
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Mézière
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Mollat
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Lapied
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Billaudel
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Courtieu
D000956:Antigens, Viral / Q000302:isolation & purification*; D002478:Cells, Cultured; D002675:Child, Preschool; D003588:Cytopathogenic Effect, Viral; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D012136:Respiratory Syncytial Viruses / Q000276:immunology* / Q000302:isolation & purification; D010253:Respirovirus Infections / Q000175:diagnosis; D013997:Time Factors
D000956:Antigens, Viral