A comparative study of mutagenic and SOS-inducing activity of biphenyls, phenanthrenequinones and fluorenones.
A total of 23 chemicals--biphenyls, phenanthrenequinones and fluorenones--were tested for mutagenicity towards Salmonella typhimurium strains TA1538, TA1535 and TA98. SOS-inducing activity of the same chemicals was studied in terms of the SOS-inducing potency in Escherichia coli PQ37, using an automated instrument controlled by a dedicated computer program for the SOS Chromotest. Of the 23 chemicals studied 14 induced His+ revertants in S. typhimurium TA1538 hisD305 (-1 frameshift); none induced His+ reversions in TA1535 (base-pair substitution). The mutagenicity of the chemicals in S. typhimurium TA98 (pKM 101) was lower than in TA1538. There was a close correlation between mutagenicity and SOS-inducing activity of fluorenones and phenanthrenequinones. None of the biphenyls tested induced SOS response and this property does not depend upon the mutagenic activity of the chemicals. SOS Chromotest is particularly valid in detecting chemicals which give rise to base-pair substitutions through SOS induction. If positive results are obtained, the Salmonella assay may be omitted. However, this test cannot replace the Ames test especially for the primary screening of mutagenicity of chemicals with unknown structure.
Institute of Chemical Physics, Academy of Sciences of the U.S.S.R., Moscow.
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S
Vasilieva
B
B
Tanirbergenov
S
S
Abilev
G
G
Migatchev
M T
MT
Huttunen
D001713:Biphenyl Compounds / Q000633:toxicity*; D004260:DNA Repair; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D005449:Fluorenes / Q000633:toxicity*; D009152:Mutagenicity Tests; D009154:Mutation; D010616:Phenanthrenes / Q000633:toxicity*; D013014:SOS Response, Genetics; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics
D001713:Biphenyl Compounds; D005449:Fluorenes; D010616:Phenanthrenes; C005399:9,10-phenanthrenequinone; C028401:9-fluorenone