Inhibition of murine cytotoxic T lymphocyte activity by a synthetic retroviral peptide and abrogation of this activity by IL.
A synthetic 17 amino acid peptide (CKS-17) homologous to a highly conserved region of human and animal retroviral transmembrane proteins was investigated for its influence on the generation of murine alloantigen-specific CTL activity in vitro. CKS-17 coupled to a carrier protein, BSA or human serum albumin, inhibited the generation of anti-allo CTL in a dose-dependent manner. Controls consisting of BSA and human serum albumin, which had undergone the coupling procedure or neurotensin, an unrelated peptide, coupled to BSA in an identical manner as CKS-17 showed no such inhibitory action. The suppression was not restricted to the Ag specificity of the CTL activity. CKS-17 exerted inhibitory effects on the early afferent phase of CTL induction. Kinetic studies showed that the suppressive activity occurred when CKS-17 was introduced to the immunologically stimulating culture concomitant with or up to 48 h after initiation of culture. Analysis of the frequency of CTL precursor cells using limiting-dilution assays revealed that CKS-17 did act to reduce the number of precursor cells. Abrogation of the inhibition of CTL activity was observed when IL-2 was introduced to the culture together with the stimulator cells. Other lymphokines, such as IL-4, exerted a similar influence to counteract this suppression.
All Children's Hospital, University of South Florida College of Medicine, St. Petersburg 33701.
M
M
Ogasawara
S
S
Haraguchi
G J
GJ
Cianciolo
M
M
Mitani
R A
RA
Good
N K
NK
Day
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D001685:Biological Factors / Q000494:pharmacology; D002478:Cells, Cultured; D016207:Cytokines; D066298:In Vitro Techniques; D036341:Intercellular Signaling Peptides and Proteins; D007376:Interleukin-2 / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology / Q000494:pharmacology*; D012191:Retroviridae Proteins / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D013997:Time Factors; D014759:Viral Envelope Proteins / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte; D001685:Biological Factors; D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins; D007376:Interleukin-2; D010455:Peptides; D012191:Retroviridae Proteins; D014759:Viral Envelope Proteins; D015847:Interleukin-4; C053692:CKS 17