Antimutagenic activity of some saponins isolated from Calendula officinalis L., C. arvensis L. and Hedera helix L.
Thirteen saponins were isolated and identified from Calendula officinalis, C. arvensis and Hedera helix. Mutagenic and antimutagenic activities of these products were investigated using a modified liquid incubation technique of the Salmonella/microsomal assay. The Salmonella tester strain TA98 +/- S9 mix was used. Screening of the antimutagenic activity was performed with a known promutagen: benzo-[a]pyrene (BaP) and a mutagenic urine concentrate from a smoker (SU). Antimutagenic activities were also compared with the activity of chlorophyllin. All the saponins were found to be non-toxic and non-mutagenic for doses of 400 micrograms. Chlorophyllin inhibited the mutagenic activities of BaP (1 microgram) and SU (5 microliters) in a dose-dependent manner. The four saponins from C. arvensis and the three saponins from H. helix showed antimutagenic activity against BaP (1 microgram) and SU (5 microliters) with a dose-response relationship. The possible mechanism of the antimutagenic activity of saponins is discussed.
Laboratoire de Pharmacognosie, Faculté de Pharmacie, Marseille, France.
R
R
Elias
M
M
De Méo
E
E
Vidal-Ollivier
M
M
Laget
G
G
Balansard
G
G
Dumenil
D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity; D002735:Chlorophyllides / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D008297:Male; D015394:Molecular Structure; D009152:Mutagenicity Tests; D009154:Mutation; D051381:Rats; D011919:Rats, Inbred Strains; D012486:Salmonella typhimurium / Q000187:drug effects; D012503:Saponins / Q000494:pharmacology* / Q000633:toxicity; D012907:Smoking / Q000652:urine
D002735:Chlorophyllides; D012503:Saponins; D001564:Benzo(a)pyrene; C007020:chlorophyllin