The three-dimensional structure and function of Escherichia coli ribosomal RNA, as studied by cross-linking techniques.
A large number of intra-RNA and RNA-protein cross-link sites have been localized within the 23S RNA from E. coli 50 S ribosomal subunits. These sites, together with other data, are sufficient to constrain the secondary structure of the 23 S molecule into a compact three-dimensional shape. Some of the features of this structure are discussed, in particular, those relating to the orientation of tRNA on the 50 S subunit as studied by site-directed cross-linking techniques. A corresponding model for the 16S RNA within the 30 S subunit has already been described, and here a site-directed cross-linking approach is being used to determine the path followed through the subunit by messenger RNA.
Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann, Berlin-Dahlem, Germany.
R
R
Brimacombe
P
P
Gornicki
B
B
Greuer
P
P
Mitchell
M
M
Osswald
J
J
Rinke-Appel
D
D
Schüler
K
K
Stade
D001483:Base Sequence; D003432:Cross-Linking Reagents; D004926:Escherichia coli / Q000235:genetics*; D008961:Models, Structural; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012335:RNA, Ribosomal / Q000235:genetics* / Q000648:ultrastructure; D012338:RNA, Ribosomal, 23S / Q000235:genetics / Q000648:ultrastructure; D012270:Ribosomes / Q000378:metabolism / Q000648:ultrastructure
D003432:Cross-Linking Reagents; D012335:RNA, Ribosomal; D012338:RNA, Ribosomal, 23S