Evaluation of the efficiency of two IgA immune complex assays.
Two IgA immune complex assays, namely the anti-IgA inhibition of binding assay (a-IgA-InhBA) and the IgA polyethylene glycol assay (IgA-PEG assay) were evaluated using IgA aggregates (AIgA) of well defined sizes. AIgA was prepared by heat treatment. After ultracentrifugation the size of AIgA was found to be in a range between 7 S and 64 S. Five AIgA pools (greater than 64 S, 44-64 S, 24-43 S, 20-24 S and 9-19 S) and a pool of monomeric IgA were prepared to test the efficiency of the two assays. The IgA-PEG assay exclusively detected very large sized AIgA (greater than 64 S), whereas the a-IgA-InhBA detected also AIgA of intermediate size (9-19 S). The sensitivity of the latter assay was found to be size dependent. After ultracentrifugation of serum samples from patients with IgA nephropathy, IgA of large and intermediate macromolecular size was detected. It is concluded that the a-IgA-InhBA is useful for the detection of circulating IgA-containing immune complexes in IgA nephropathy.
Osawa
H
H
Department of Nephrology, University Hospital Leiden, The Netherlands.
Daha
M R
MR
Van Es
L A
LA
D000936:Antigen-Antibody Complex / Q000032:analysis*; D011232:Chemical Precipitation; D005922:Glomerulonephritis, IGA / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis*; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D008970:Molecular Weight; D011092:Polyethylene Glycols
D000936:Antigen-Antibody Complex; D007070:Immunoglobulin A; D046911:Macromolecular Substances; D011092:Polyethylene Glycols