A kinetic assay for urea in undiluted urine specimens.
We describe a kinetic assay for quantifying urea in undiluted urine samples. The assay linearity extends to urea concentrations of 400 mmol/L, is free from interference from blood (10 mL/L) or bilirubin (1 mmol/L), is sufficiently precise for routine use, and correlates well with an established method for assay of diluted urine samples.
Scott
P
P
Department of Clinical Biochemistry, Addenbrookes Hospital, Cambridge, U.K.
Maguire
G A
GA
D001407:Bacillus / Q000201:enzymology; D002021:Buffers; D006801:Humans; D007700:Kinetics; D013048:Specimen Handling; D014508:Urea / Q000652:urine*; D014510:Urease
D002021:Buffers; D014508:Urea; D014510:Urease