Synovial fluid pH, cytologic characteristics, and gentamicin concentration after intra-articular administration of the drug in an experimental model of infectious arthritis in horses.
Chemical and cytologic effects and bactericidal activity of gentamicin in septic synovial fluid were evaluated in an experimental model of infectious arthritis in horses. Septic arthritis was induced by inoculation of approximately 7.5 X 10(6) colony-forming units of Escherichia coli into 1 antebrachiocarpal joint in each of 16 clinically normal adult horses. Clinical signs of septic arthritis were evident 24 hours after inoculation. Horses were allotted to 3 groups: group-1 horses (n = 5) each were given 150 mg of gentamicin (50 mg/ml; 3 ml) intra-articularly (IA); group-2 horses (n = 5) each were given 2.2 mg of gentamicin/kg of body weight, IV, every 6 hours; and group-3 horses (n = 6) each were given buffered gentamicin, consisting of 3 mEq of sodium bicarbonate (1 mEq/ml; 3 ml) and 150 mg of gentamicin (50 mg/ml; 3 ml), IA. Synovial fluid specimens were obtained at posttreatment hour (PTH) 0, 0.25, 1, 4, 8, 12, and 24 via an indwelling intra-articular catheter. Synovial fluid pH was evaluated at PTH 0, 0.25, and 24. Microbiologic culture and cytologic examination were performed on synovial fluid specimens obtained at PTH 0 and 24, and gentamicin concentration was measured in all synovial fluid specimens. At PTH 0, E coli was isolated from synovial fluid specimens obtained from all horses. Synovial fluid pH was lower (range, 7.08 to 7.16) and WBC count was higher (range, 88,000 to 227,200 cells/microliters) and predominantly neutrophilic (95 to 99%) at PTH 0 than before inoculation.(ABSTRACT TRUNCATED AT 250 WORDS)
Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis 95616.
K C
KC
Lloyd
S M
SM
Stover
J R
JR
Pascoe
P
P
Adams
D000818:Animals; D001170:Arthritis, Infectious / Q000188:drug therapy / Q000662:veterinary*; D004926:Escherichia coli / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000662:veterinary*; D005260:Female; D005839:Gentamicins / Q000008:administration & dosage / Q000032:analysis / Q000494:pharmacology*; D006734:Horse Diseases / Q000188:drug therapy* / Q000378:metabolism; D006736:Horses; D006863:Hydrogen-Ion Concentration; D007270:Injections, Intra-Articular / Q000662:veterinary; D007275:Injections, Intravenous / Q000662:veterinary; D008297:Male; D013582:Synovial Fluid / Q000032:analysis* / Q000166:cytology / Q000382:microbiology; D013997:Time Factors
D005839:Gentamicins