Cloning of the ethidium efflux gene from Escherichia coli.
The gene specifying the ethidium efflux system of Escherichia coli has been cloned on a 3.2 kbp HindIII fragment and located on a 1.2 kbp fragment within this. Cross-resistance studies indicate that the system has a broad specificity for monovalent cations and the gene shows no hybridisation with similar genes found in Staphylococci.
Department of Applied Biology, University of Hull, U.K.
A S
AS
Purewal
I G
IG
Jones
M
M
Midgley
D015139:Blotting, Southern; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D004996:Ethidium / Q000378:metabolism / Q000494:pharmacology*; D005798:Genes, Bacterial; D009154:Mutation; D010957:Plasmids; D015183:Restriction Mapping; D013045:Species Specificity; D013210:Staphylococcus / Q000235:genetics
D004996:Ethidium