Virulent Escherichia coli strains for chicks bind fibronectin and type II collagen.
125I-fibronectin and 125I-collagen (type II) binding was detected in Escherichia coli strains isolated from chickens and poults. High fibronectin binding-strains also bind the 29 kD aminoterminal fragment of fibronectin. Binding properties in strain CK28 were partially characterized. The highest binding of 125I-fibronectin and 125I-collagen for strain CK28 was obtained with bacteria grown at 33 degrees C. Binding of 125I-fibronectin, its 125I-29 kD fragment, and 125I-collagen, was very rapid, reaching a maximum in 5 min. Binding of 125I-fibronectin and 125I-collagen was considerably inhibited by preincubation of bacteria with unlabelled fibronectin and unlabelled type I collagen respectively, but not inhibited with human immunoglobulin G or bovine serum albumin. Inhibition experiments showed that the reversibility of 125I-fibronectin binding was estimated at approximately 50%, while reversibility for 125I-collagen binding was higher than 90%. Receptors for fibronectin, its 29 kD fragment, and collagen were released from the bacterial surface by treatment at different temperatures, and surface material released at 100 degrees C inhibited binding. There was cross-inhibition for both fibronectin and collagen binding when unlabelled fibronectin and unlabelled collagen were used as inhibitors, suggesting that binding receptors for both proteins may be closely located.
Departamento de Microbiología y Parasitología, Universidad de Santiago, Facultad de Veterinaria, Lugo, Spain.
E A
EA
Gonzalez
J
J
Blanco
S B
SB
Baloda
G
G
Fröman
M
M
Dho
J P
JP
Lafont
T
T
Wadström
D000818:Animals; D001665:Binding Sites; D002645:Chickens; D003094:Collagen / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D005353:Fibronectins / Q000378:metabolism*; D006384:Hemagglutination; D011201:Poultry Diseases / Q000382:microbiology*; D013499:Surface Properties; D014774:Virulence
D005353:Fibronectins; D003094:Collagen