Relaxin stimulates the synthesis and release of prorenin from human decidual cells: evidence for autocrine/paracrine regulation.
Porcine relaxin caused a time- and concentration-dependent increase in the release of renin from decidual cells cultured over a 96 h period. The increase in renin release occurred 24-48 h after exposure and was maximal at 48-72 h. Half-maximal stimulation occurred at a relaxin concentration of 5 ng/ml, and maximal stimulation (250-270%) occurred at concentrations greater than or equal to 10 ng/ml. At each time, greater than 95% of the renin released into the medium was in the form of prorenin. The stimulation of renin release was paralleled by a stimulation of cellular renin content and was completely inhibited by cycloheximide, indicating that relaxin also stimulated renin synthesis. Since renin is present in both cytotrophoblast and decidual cells, these results suggest a paracrine and/or autocrine relationship between relaxin- and prorenin-secreting cells.
Department of Pharmacology, Univ. of Kansas Medical Center, Kansas City 66103.
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Poisner
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Thrailkill
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Poisner
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Handwerger
D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003656:Decidua / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004792:Enzyme Precursors / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D012065:Relaxin / Q000494:pharmacology*; D012083:Renin / Q000096:biosynthesis*; D013997:Time Factors
D004792:Enzyme Precursors; D012065:Relaxin; D003513:Cycloheximide; D012083:Renin