Purification and some characteristics of chicken liver L-2-hydroxyacid oxidase A.
The isozyme A of L-2-hydroxyacid oxidase is a peroxisomal flavoenzyme that catalyzes the oxidation of short-chain aliphatic L-2-hydroxyacids in many tissues of higher organisms. A new purification procedure allowed us to obtain a 1400-fold purified enzyme from chicken liver. The N-terminal amino acid of the polypeptide chain was found to be blocked as that of spinach glycolate oxidase, contrastingly with that of rat kidney isozyme B. Its amino acid composition was comparable to that of other known L-2-hydroxyacid oxidases. Despite different substrate specificity, some immunological identity was observed between chicken liver L-2-hydroxyacid isozyme A and rat kidney isozyme B.
Centre de Biochimie et de Biologie Moléculaire du Centre National de la Recherche Scientifique, Marseille, France.
L
L
Dupuis
J
J
De Caro
P
P
Brachet
A
A
Puigserver
D000429:Alcohol Oxidoreductases / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D015153:Blotting, Western; D002645:Chickens; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008715:Methionine / Q000031:analogs & derivatives / Q000378:metabolism; D008830:Microbodies / Q000201:enzymology; D008970:Molecular Weight
D000596:Amino Acids; C008391:alpha-hydroxy-gamma-methylmercaptobutyric acid; D008715:Methionine; D000429:Alcohol Oxidoreductases; C021380:L-2-hydroxyacid oxidase