[Comparison between culture method and direct immunofluorescence test for detection of Chlamydia trachomatis and follow-up survey of antigen positive patients].
C. trachomatis isolation by culture method using McCoy cells and direct immunofluorescence test (DFA) were carried out on specimens collected from patients with suspected chlamydial infections and the results were compared. Follow-up survey was also made on C. trachomatis positive patients. The results are summarized as follows: 1) One thousand and eighty-six specimens were examined. Positive-coincidence ratio obtained from both methods was 80% and negative-coincidence ratio was 92%. The antigen detection by the DFA exceeded a little bit in positive ratio than that by the culture method. 2) C. trachomatis was positively detected in 31 of 94 men (33%) and 5 of 27 (17%) women during periods from 7 days to 2.5 years in follow-up examinations.
Bessho
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Department of Microbiology, Kawasaki Medical School, Kurashiki, Japan.
Matsumoto
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D000942:Antigens, Bacterial / Q000032:analysis*; D001431:Bacteriological Techniques; D002478:Cells, Cultured; D002690:Chlamydia Infections / Q000276:immunology / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000276:immunology / Q000302:isolation & purification*; D005260:Female; D005455:Fluorescent Antibody Technique; D005500:Follow-Up Studies; D006801:Humans; D008297:Male
D000942:Antigens, Bacterial