[Monoclonal antibodies to ceruloplasmin and their use for immunoenzyme assay of this protein].
The hybridoma technology was used to produce monoclonal antibodies to human ceruloplasmin. The antibodies were found to be related to Ig G1. Using these monoclonal antibodies for PAP-ELISA of ceruloplasmin, it was possible to determine 10(-11) M of the protein. Monoclonal antibodies coupled to CNBr-Sepharose were used for the rapid one-stage purification of ceruloplasmin from human placental serum. Ceruloplasmin obtained by this method contained no type 2 copper normally detected in protein preparations by conventional methods.
T B
TB
Megrabian
Z B
ZB
Megrabian
E V
EV
Sidorova
R M
RM
Nalbandian
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002570:Ceruloplasmin / Q000032:analysis / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D002570:Ceruloplasmin
Monoklonal'nye antitela k tseruloplazminu i ikh ispol'zovanie dlia immunofermentnogo analiza étogo belka.