Assessment of urinary rat beta 2-microglobulin by enzyme immunoassay.
An enzyme immunoassay (EIA) was developed using unlabelled and peroxidase-labelled rabbit antibodies to assess urinary rat beta 2-microglobulin (beta 2-m) excretion. It consisted in the adsorption of rabbit anti-rat beta 2-m immunoglobulin to a polystyrene surface, the addition of beta 2-m samples or standard and the addition of peroxidase-labelled rabbit anti-rat beta 2-m immunoglobulin. After addition of hydrogen peroxide and o-phenylenediamine, the enzyme activity of the solid phase was measured photometrically at 490 nm. Analytical recovery of pure beta 2-m in urine was 102%. From determinations made on normal female and male rats, the ranges of 24-h urine beta 2-m individually excreted were found to be 0.84-4.77 and 3.7-17.3 micrograms, respectively. The means +/- SEM were 2.49 +/- 0.17 and 10.2 +/- 0.55 micrograms. EIA was of value in evidencing the tubule-damaging properties of sodium chromate and hexachloro-1,3-butadiene in rats.
Institut National de Recherche et de Sécurité, Vandoeuvre, France.
G
G
Morel
J P
JP
Payan
J
J
de Ceaurriz
D000818:Animals; D015415:Biomarkers / Q000652:urine; D002070:Butadienes / Q000633:toxicity; D002840:Chromates / Q000633:toxicity; D005260:Female; D005659:Fungicides, Industrial / Q000633:toxicity; D007124:Immunoenzyme Techniques; D007279:Injections, Subcutaneous; D008297:Male; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D017670:Sodium Compounds; D001613:beta 2-Microglobulin / Q000652:urine*
D015415:Biomarkers; D002070:Butadienes; D002840:Chromates; D005659:Fungicides, Industrial; D017670:Sodium Compounds; D001613:beta 2-Microglobulin; C028982:sodium chromate(VI); C001335:hexachlorobutadiene