Induction of differentiation of human leukemia cells by various combinations of cytokines and low-molecular-weight inducers.
To explore agents for differentiation therapy of leukemias, various combinations of cytokines and low-molecular-weight inducers were examined for differentiation-inducing activity toward three kinds of human leukemia-derived cell lines. The strongest differentiation inducing activity on promyelocytic HL60 cells and histiocytic U937 cells was obtained by combining recombinant tumor necrosis factor (rTNF), interferon-gamma (IFN-gamma), retinoic acid (RA), and 1 alpha,25-dihydroxyvitamin D3 (1 alpha,25(OH)2D3). For myeloblastic ML1 cells, the combination of rTNF, IFN-gamma, and RA had the strongest differentiation-inducing activity.
Nakaya
K
K
School of Pharmaceutical Sciences, Shawa University, Tokyo, Japan.
Chou
S
S
Deguchi
Y
Y
Nakamura
Y
Y
D001685:Biological Factors / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D016207:Cytokines; D006801:Humans; D007938:Leukemia / Q000473:pathology*; D008970:Molecular Weight; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology
D001685:Biological Factors; D016207:Cytokines