Glial fibrillary acidic protein (GFAP) immunoreactivity in rabbit retina: effect of fixation.
The binding of monoclonal and polyclonal antibodies to glial fibrillary acidic protein (GFAP) antigenic sites in the rabbit retina was shown to be sensitive to aldehyde fixation. In chemically unfixed retina, the polyclonal anti-GFAP labeled Müller cells, astrocytes, and unidentified profiles in the outer plexiform layer; the monoclonal anti-GFAP labeled Müller cell endfeet and astrocytes only. The outer plexiform layer label with the polyclonal antibody was lost after fixation for 1 hr in 1% paraformaldehyde; elsewhere, the label was reduced. Fixation also reduced labeling by the monoclonal antibody. Such fixation sensitivity may underlie the different patterns reported for retinal GFAP immunoreactivity in the literature.
Neuroscience Research Institute, University of California, Santa Barbara 93106.
D K
DK
Vaughan
P A
PA
Erickson
S K
SK
Fisher
D000818:Animals; D000918:Antibody Specificity; D001666:Binding Sites, Antibody / Q000276:immunology; D015153:Blotting, Western; D005404:Fixatives; D005557:Formaldehyde; D005904:Glial Fibrillary Acidic Protein / Q000276:immunology / Q000378:metabolism*; D006651:Histocytochemistry; D006652:Histological Techniques; D011108:Polymers; D011817:Rabbits; D012160:Retina / Q000378:metabolism*; D013997:Time Factors
D005404:Fixatives; D005904:Glial Fibrillary Acidic Protein; D011108:Polymers; D005557:Formaldehyde; C003043:paraform