Neutral endopeptidase from nuchal ligament of fetal calves.
The nuchal ligament of unborn calves contains a neutral endopeptidase that is biochemically and immunologically similar to the neutral endopeptidase (NEP), or enkephalinase, from human kidney. Enzymatic activity was inhibited more than 90% by phosphoramidon (1 microM). The specific activity in membrane fractions, as determined by hydrolysis of the dansylated substrate, DAPGN, was similar in tissue from fetuses of gestational ages ranging from 100 to 280 days. NEP activity in adult ligament tissue, however, was less than 10% of that in fetal tissue. Fibroblasts dissociated from ligament tissue by collagenase displayed less NEP activity than did preparations of intact ligament, and activity was even lower in cultured cells. By contrast, fibroblasts cultured from fetal calf lungs had NEP activity comparable to that in the ligament tissue. When ligament fibroblasts were cultured on subcellular matrices derived from fetal lung fibroblasts the NEP activity increased relative to those cultured on plastic alone. These studies confirm the presence of neutral endopeptidase (NEP) in the nuchal ligament of the fetal calf. The consistent activity through a range of gestational ages and the influence of the subcellular matrix suggest that this enzyme might be involved in growth of the ligament during fetal life.
Department of Biochemistry, University of Texas Health Center, Tyler 75710.
A R
AR
Johnson
L D
LD
Gray
E
E
Youngblood
J
J
Sullivan
D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000502:physiology; D005333:Fetus / Q000201:enzymology; D005347:Fibroblasts / Q000201:enzymology / Q000648:ultrastructure; D005865:Gestational Age; D008022:Ligaments / Q000196:embryology / Q000201:enzymology*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D009333:Neck; D015260:Neprilysin / Q000378:metabolism*
D015260:Neprilysin