Cloning and characterization of the iron-sulfur subunit gene of succinate dehydrogenase from Saccharomyces cerevisiae.
We describe the cloning and characterization of the complete gene for the iron-sulfur protein subunit of succinate dehydrogenase (EC 1.3.99.1) from Saccharomyces cerevisiae. The promoter and coding sequence have been cloned into an Escherichia coli-yeast shuttle vector. The cloned gene complements the defect in a succinate dehydrogenase-deficient yeast mutant isolated by us, and gene expression is fully responsive to induction by glucose deprivation, indicating that the promoter is intact.
Lombardo
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A
Department of Biology, University of California, San Diego, La Jolla 92093.
Carine
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K
Scheffler
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IE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D003001:Cloning, Molecular; D015342:DNA Probes; D015870:Gene Expression; D005800:Genes, Fungal; D005822:Genetic Vectors; D007506:Iron-Sulfur Proteins / Q000235:genetics*; D046911:Macromolecular Substances; D008667:Metalloproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013385:Succinate Dehydrogenase / Q000235:genetics*
D015342:DNA Probes; D007506:Iron-Sulfur Proteins; D046911:Macromolecular Substances; D008667:Metalloproteins; D013385:Succinate Dehydrogenase