Periodic biosynthesis of the human M-phase promoting factor catalytic subunit p34 during the cell cycle.
The product of the CDC2Hs gene is the protein kinase subunit of the M-phase promoting factor, which is required for entry into mitosis. The activity of this kinase is regulated in a cell cycle-dependent manner by reversible phosphorylation and through association with other proteins. We report here that in HeLa cells, the abundance of the CDC2Hs mRNA and the rate of synthesis of the encoded protein, p34, vary in a cell cycle-dependent manner.
McGowan
C H
CH
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Russell
P
P
Reed
S I
SI
D016203:CDC2 Protein Kinase; D002453:Cell Cycle; D006367:HeLa Cells / Q000166:cytology / Q000201:enzymology; D006801:Humans; D007399:Interphase; D007700:Kinetics; D046911:Macromolecular Substances; D008938:Mitosis; D008954:Models, Biological; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000235:genetics; D010766:Phosphorylation; D011494:Protein Kinases / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000235:genetics
D046911:Macromolecular Substances; D010750:Phosphoproteins; D012333:RNA, Messenger; D011494:Protein Kinases; D016203:CDC2 Protein Kinase