In vivo transport kinetics and distribution of taurocholate by rat ileum and jejunum.
The transport kinetics and distribution of taurocholate (TC) from the ileum and jejunum were evaluated in anesthetized Sprague-Dawley rats. Uptake and transport kinetics were determined after simultaneous administration of equimolar solutions of [3H]taurocholic acid ([3H]TC) in the ileum and [24-14C]taurocholic acid ([14C]TC) in the jejunum of anesthetized rats. At TC concentrations between 0.3 and 15 mM, total ileal absorption exceeded jejunal absorption 15- to 25-fold. The apparent Km and Vmax for ileal absorption of TC were 5.6 mM and 65.5 nmol.min-1.cm-1, respectively. Tissue distribution studies following uptake from the ileum and jejunum were done with 75Se-labeled homocholic acid taurine (75Se-HCAT). In the ileum, 82% of the 75Se-HCAT removed from the lumen was found in the bile, 10.6% was found in the ileal wall, and 7.3% was found in the liver. In the jejunum, 24.1% was found in bile, 0.6% was found in the liver, and 75.3% remained in the jejunal wall. These data show that the ileum is much more efficient and better equipped than the jejunum to take up and transport TC at concentrations up to 15 mM.
Division of Pharmacology, Burroughs Wellcome Company, Research Triangle Park, North Carolina 27709.
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D000818:Animals; D001692:Biological Transport; D001693:Biological Transport, Active; D002250:Carbon Radioisotopes; D007082:Ileum / Q000502:physiology*; D007408:Intestinal Absorption; D007583:Jejunum / Q000502:physiology*; D007700:Kinetics; D008297:Male; D009130:Muscle, Smooth / Q000502:physiology; D011865:Radioisotope Dilution Technique; D051381:Rats; D011919:Rats, Inbred Strains; D012644:Selenium Radioisotopes; D013656:Taurocholic Acid / Q000378:metabolism*; D014316:Tritium
D002250:Carbon Radioisotopes; D012644:Selenium Radioisotopes; D014316:Tritium; D013656:Taurocholic Acid