Bacterial epididymitis in the rat: a model for assessing the impact of acute inflammation on epididymal antibiotic penetration.
A rat model of bacterial epididymitis was developed and characterized for use in assessing the impact of acute epididymal inflammation on antibiotic penetration into the epididymis. A 0.2 ml. intratesticular injection of a 0.5 McFarland standard suspension of E. coli resulted in histologically confirmed acute epididymitis in all animals studied. Inflammatory changes were detectable as early as 24 hours following inoculation and were progressive to the last assessment point at 11 days. Early testicular infarction was observed in association with epididymal inflammation. Serial transcrotal ultrasounds of infected animals showed progressive increase in epididymal size and a late decrease in testicular size. Serum and epididymal drug concentrations were assayed following a single dose of the antibiotic amdinocillin. Fifteen minutes following the peak serum level, the drug concentration in infected epididymis was 2.3-fold higher than the contralateral, non-infected epididymis. These data suggest that acute inflammation enhances antibiotic penetration into the infected epididymis. The model described provides a rapid, reproducible method to study epididymal drug delivery in normal and diseased states.
Department of Urology, University of Washington, Seattle.
W A
WA
See
T O
TO
Taylor
L A
LA
Mack
T A
TA
Tartaglione
K E
KE
Opheim
R E
RE
Berger
D000560:Amdinocillin / Q000493:pharmacokinetics* / Q000627:therapeutic use; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004822:Epididymis / Q000473:pathology; D004823:Epididymitis / Q000188:drug therapy*; D004927:Escherichia coli Infections / Q000188:drug therapy*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014463:Ultrasonography
D000560:Amdinocillin