Statin expression in the untreated and SarCNU-exposed human glioma cell line, SK-MG-1.
Cytokinetic analyses of gliomas and other neoplasms rely exclusively on the use of proliferation-dependent markers such as [3H]-thymidine and BuDR incorporation and the detection of growth-dependent proteins such as proliferating cell nuclear antigen (PCNA) and Ki-67. In normal tissues, the monoclonal antibody S-44 recognizes statin, a nuclear protein expressed only in nonproliferating cells. In the present study, indirect immunofluorescence microscopy using S-44 identified nuclear statin in 5.9% of a population of untreated human SK-MG-1 glioma cells in vitro. Incremental doses of the alkylating agent sarcosinamide chloroethylnitrosourea (SarCNU) induced a linear increase in the fraction of statin-positive SK-MG-1 cells. Labeling of nuclear statin with the monoclonal antibody S-44 may be a potentially useful marker of the cytotoxic effects of anticancer drugs in gliomas and other neoplastic tissues.
Schipper
H M
HM
Department of Neurology, Lady Davis Institute for Medical Research, Sir Mortimer B. Davis Jewish General Hospital, Montreal, Quebec, Canada.
Skalski
V
V
Panasci
L C
LC
Wang
E
E
D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D002330:Carmustine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D018797:Cell Cycle Proteins; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D005455:Fluorescent Antibody Technique; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D020648:Peptide Elongation Factor 1; D011237:Predictive Value of Tests; D011506:Proteins / Q000276:immunology / Q000378:metabolism*; D012044:Regression Analysis; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D014410:Tumor Stem Cell Assay
D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; D018797:Cell Cycle Proteins; C503234:EEF1A2 protein, human; D020648:Peptide Elongation Factor 1; D011506:Proteins; C044565:2-((((2-chloroethyl)nitrosoamino)carbonyl)amino)propanamide; D002330:Carmustine