The use of charge-coupled devices in the quantitative evaluation of images, on photographic film or membranes, obtained following electrophoretic separation of DNA fragments.
Charge-coupled devices can provide an excellent means for the quantification of DNA band images on membranes and photographic film. However, proper consideration must be given to important parameters such as signal-to-noise ratio and resolution, especially in applications which demand the use of large format media. When employed in the direct imaging of chemiluminescent blots, the charge-coupled device can provide equal or better sensitivity than that obtained by indirect methods using film, with the additional advantages of wide dynamic range and freedom from the vagaries of film processing.
Boniszewski
Z A
ZA
Amersham International plc, Little Chalfont, Buckinghamshire, UK.
Comley
J S
JS
Hughes
B
B
Read
C A
CA
D004247:DNA / Q000302:isolation & purification*; D004586:Electrophoresis; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008567:Membranes, Artificial; D010781:Photography; D011877:Radionuclide Imaging; D012680:Sensitivity and Specificity; D014963:X-Ray Intensifying Screens
D008567:Membranes, Artificial; D004247:DNA