Rapid release of endothelin and ATP from isolated aortic endothelial cells exposed to increased flow.
Freshly harvested rabbit aortic endothelial cells on filters were exposed to two 3 min periods of a sixfold increase in flow rate of the perfusion buffer. This led to an increase in the levels of endothelin and ATP in the perfusate; arginine vasopressin remained at the basal level. Less ATP was released on the second exposure to high flow; however, endothelin release was not diminished. Using immunohistochemical techniques, endothelin and arginine vasopressin were localised in the same population of endothelial cells; endothelin and vasopressin were present in approximately 90% and 70% of endothelial cells, respectively, which suggests that there is some co-localisation. This is the first time that a stimulation has been shown to produce rapid release of endothelin.
Department of Anatomy and Developmental Biology, University College, London, UK.
P
P
Milner
P
P
Bodin
A
A
Loesch
G
G
Burnstock
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001011:Aorta / Q000378:metabolism; D001127:Arginine Vasopressin / Q000378:metabolism; D002478:Cells, Cultured; D016232:Endothelins; D004730:Endothelium, Vascular / Q000378:metabolism*; D008297:Male; D010455:Peptides / Q000378:metabolism*; D010477:Perfusion; D011817:Rabbits
D016232:Endothelins; D010455:Peptides; D001127:Arginine Vasopressin; D000255:Adenosine Triphosphate