Transfection of cultured fish cells RBCF-1 with exogenous oncogene and their resistance to malignant transformation.
1. Plasmids bearing the G418-resistant gene neo were transfected into cultured fish cells RBCF-1 by electroporation at an efficiency comparable to that in NIH3T3 cells. 2. Transfection of plasmids bearing both neo and activated human c-Ha-ras into NIH3T3 and RBCF-1 cells resulted in the malignant transformation of the former but not of the latter cells.
Laboratory of Radiation Biology, Faculty of Science, University of Tokyo, Japan.
K
K
Hayasaka
M
M
Sato
H
H
Mitani
A
A
Shima
D000818:Animals; D000900:Anti-Bacterial Agents; D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D004351:Drug Resistance / Q000235:genetics; D011905:Genes, ras; D005839:Gentamicins; D006054:Goldfish; D006801:Humans; D051379:Mice; D014162:Transfection; D001749:Urinary Bladder Neoplasms
D000900:Anti-Bacterial Agents; D005839:Gentamicins; C010680:antibiotic G 418