Improved method for identifying alpha 1-antitrypsin Pi M subtypes by isoelectric focusing in agarose.
We describe an improved method for the classification of alpha 1-antitrypsin variants by isoelectric focusing in agarose. Identification of the three Pi M subtypes can now be made by using a narrow-range carrier ampholyte (pH 4.2-4.9) and pretreating serum with dithioerythritol-iodoacetic acid to enhance band resolution. Phenotype results for two groups of Pi M homo- and heterozygotes are compared to illustrate the improved accuracy of the new method.
Nowicki
M L
ML
Department of Laboratory Medicine and Pathology, University of Minnesota Hospital and Clinic, Minneapolis 55455.
Freier
E F
EF
D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000235:genetics*; D010641:Phenotype; D012685:Sepharose; D000515:alpha 1-Antitrypsin / Q000145:classification / Q000235:genetics*
D007527:Isoenzymes; D000515:alpha 1-Antitrypsin; D012685:Sepharose