ERD2, a yeast gene required for the receptor-mediated retrieval of luminal ER proteins from the secretory pathway.
Resident proteins of the ER lumen carry a specific tetrapeptide signal (KDEL or HDEL) that prevents their secretion. We have previously described the isolation of yeast mutants that fail to retain such resident proteins within the cell. Here we describe ERD2, a gene required for retention. It encodes a 26 kd integral membrane protein whose abundance determines the efficiency and capacity of the retention system. Reduced expression of ERD2 leads to secretion of proteins bearing the HDEL signal, whereas overexpression of ERD2 improves retention both in wild-type cells and in other mutants. These results are consistent with other evidence that ERD2 encodes the HDEL receptor (see accompanying paper). The gene is also required, perhaps indirectly, for normal protein transport through the Golgi, and hence for growth. We discuss possible roles for ERD2 in the secretory pathway.
MRC Laboratory of Molecular Biology, Cambridge, England.
J C
JC
Semenza
K G
KG
Hardwick
N
N
Dean
H R
HR
Pelham
D000595:Amino Acid Sequence; D001483:Base Sequence; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D005656:Fungal Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D005800:Genes, Fungal; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D011487:Protein Conformation; D018000:Receptors, Peptide; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins
C064922:ERD2 protein, S cerevisiae; D005656:Fungal Proteins; D008565:Membrane Proteins; D018000:Receptors, Peptide; D029701:Saccharomyces cerevisiae Proteins