Purification of the upstream element factor of the adenovirus-2 major late promoter from HeLa and yeast by sequence-specific DNA affinity chromatography.
The purification to homogeneity of the Adenovirus-2 major late promoter (MLP) upstream element factor (UEF), a sequence specific transcription factor, which binds to upstream elements of various class B (II) genes, is reported. The protein was purified from HeLa cells and also from the yeast Saccharomyces cerevisiae, by using sequence-specific DNA affinity chromatography. The human (UEFh, 45,000 dalton) and the yeast (UEFy, 60,000 dalton) proteins protect the same sequences over the MLP-IVa2 intergenic region: the MLP-UE (from nucleotide -49 to -67) and the IVa2-UE (from nucleotide -98 to -122 relative to the MLP initiation site). Both proteins have a higher affinity for the MLP-UE than for the IVa2-UE.
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Institut de Chimie Biologique, Faculté de Médecine, Strasbourg, France.
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Moncollin
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Gerard
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JM
Egly
D000260:Adenoviruses, Human / Q000235:genetics*; D001665:Binding Sites; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004247:DNA / Q000032:analysis*; D004268:DNA-Binding Proteins / Q000302:isolation & purification*; D003850:Deoxyribonuclease I; D006367:HeLa Cells; D006801:Humans; D008970:Molecular Weight; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014157:Transcription Factors / Q000302:isolation & purification*; D014764:Viral Proteins
D004268:DNA-Binding Proteins; C051096:MLTF protein, adenovirus; D014157:Transcription Factors; D014764:Viral Proteins; D004247:DNA; D003850:Deoxyribonuclease I