Human tumor cell lines express low levels of oncomodulin.
Different human carcinoma cell lines were screened for the presence of Ca2(+)-binding oncomodulin. A specific polyclonal antibody was raised against a synthetic peptide (amino acids 99-108) of oncomodulin coupled to hemocyanin. Extracts of tumor cell lines (several human, one rat) were analyzed for the presence of oncomodulin by immunoblotting. A strong immunoreaction of oncomodulin was obtained in chemically transformed rat fibroblasts (T14c) in contrast to all human tumor cell lines investigated, where no immunoreaction was obtained. These results suggest that oncomodulin cannot be used in diagnostics of human tumors.
Department of Pediatrics, University of Zürich, Switzerland.
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S
Huber
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M
Leuthold
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EW
Sommer
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CW
Heizmann
D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002277:Carcinoma / Q000378:metabolism*; D006801:Humans; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D009363:Neoplasm Proteins / Q000276:immunology / Q000378:metabolism*; D010446:Peptide Fragments / Q000276:immunology; D014407:Tumor Cells, Cultured
D002135:Calcium-Binding Proteins; D009363:Neoplasm Proteins; D010446:Peptide Fragments; C034733:oncomodulin