Autoagglutination and the specificity of the indirect fluorescent antibody test applied to the identification of Taylorella equigenitalis.
Because the first strains of Taylorella equigenitalis isolated in the Netherlands autoagglutinated, identification was difficult. The source of carbon dioxide to create a carbon dioxide atmosphere for incubation influenced the emulsifiability of these strains. Strains were emulsifiable when cultivated in a carbon dioxide incubator (7 per cent carbon dioxide in air), but were autoagglutinable when cultivated in a candle jar, or in a jar with a carbon dioxide system or anaerobic system without the palladium catalyst. When strains autoagglutinated, they were identified by the indirect fluorescent antibody test. Although strains of other bacterial species, in particular Pasteurella haemolytica, also showed fluorescence, which was partly caused by autofluorescence, the indirect fluorescent antibody test appeared to be a reliable additional test for identifying T equigenitalis.
Central Veterinary Institute, Lelystad, The Netherlands.
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Ter Laak
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Wagenaars
D000371:Agglutination; D000372:Agglutination Tests; D000818:Animals; D005455:Fluorescent Antibody Technique; D015661:Pasteurellaceae / Q000145:classification / Q000302:isolation & purification*; D011237:Predictive Value of Tests