[The use of photographic materials as a dry reagent for determining the peroxidase activity of biological preparations].
A new method is reviewed for measuring the peroxidase activity of biopreparations using light quantum-sensitized photographic material as a detector. The method involves the combination of the reactions of peroxidase oxidation of hydroquinone, a constituent part of the developing solutions, by hydrogen peroxide and photochemical interaction of the remaining hydroquinone with silver halogenides of the photoemulsion layer. Visually, up to 2 ng/ml of horse radish peroxidase is detectable. On photometry of the spots obtained, the limit of peroxidase demonstration amounts to 0.6 ng/ml. The data obtained during measurements of the peroxidase activity of the saliva, blood serum, plant extracts and immunoenzymic conjugates are provided.
Aronbaev
D M
DM
Varfolomeev
S D
SD
Krivoruchko
V I
VI
Simonova
M V
MV
D006801:Humans; D006873:Hydroquinones; D007124:Immunoenzyme Techniques; D010544:Peroxidases / Q000097:blood; D010781:Photography; D012463:Saliva / Q000201:enzymology
D006873:Hydroquinones; D010544:Peroxidases
Primenenie fotomaterialov v kachestve "sukhogo" reagenta dlia opredeleniia peroksidaznoĭ aktivnosti biopreparatov.