[Spontaneous fluctuation of extracellular matrix molecules on the surface of human skin fibroblasts in culture: flow cytometric analysis].
The evolution of five surface markers (CD 10, fibronectin, collagens I, III, IV) was analysed in cytofluorometry after immunolabelling of cultured human skin fibroblasts. The expression of the CD10 molecule and collagen IV remained stable. On the contrary, there was a spontaneous variation of the cell surface distribution of fibronectin and collagens I and III. This variation was periodical and followed the subculture rhythm. After every passage, the density of matrix components increased at the cell surface. The maximum of the density was achieved on confluent fibroblasts then decreased until the next passage. The fibronectin and collagens I and III expression was permanently altered on senescent fibroblasts. Modifications of the expression of extracellular matrix proteins on the fibroblasts surface were closely linked with cellular density and senescence.
Laboratoire d'Immunologie du CRTS, Gerland, France.
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Andréoni
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Rigal
D002478:Cells, Cultured; D003094:Collagen / Q000502:physiology; D005347:Fibroblasts / Q000502:physiology*; D005353:Fibronectins / Q000502:physiology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000502:physiology*; D013997:Time Factors
D005353:Fibronectins; D008565:Membrane Proteins; D003094:Collagen
Fluctuations spontanées des molécules de la matrice extracellulaire à la surface des fibroblastes cutanés humains en culture: analyse cytofluorométrique.