[Chromosomal localization of transforming oncogene BGC-Ha-ras in gastric cancer cell line].
BGC-Ha-ras, isolated from the human gastric cancer cell line BGC-823 and activated by point mutation, is a transforming oncogene. To study whether this oncogene can still be located in the original position of chromosome 11 of BGC-823 cell line, chromosomal in situ hybridization method was used. The results showed that 31% silver grains were localized on chromosome 11, 83% of which on the 11p15 region, and in addition, 10% grains on distal end of the short arm of the other abnormal chromosome. Such results are significant in further study of the relation between gene localization and carcinogenesis.
Beijing Institute for Cancer Research.
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Hou
D002461:Cell Line, Transformed; D002871:Chromosome Banding; D002880:Chromosomes, Human, Pair 11; D011905:Genes, ras; D006801:Humans; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured