Presynaptic nicotinic receptors involved in release of noradrenaline and ATP from the prostatic portion of the rat vas deferens.
A differential response to cholinomimetic agonists in epididymal and prostatic portions of rat vas deferens was characterized. The prostatic portion was less sensitive to acetylcholine and carbachol than the epididymal portion. The contraction induced by cholinomimetic agonists was inhibited in the epididymal portion by atropine (1.0-3.0 nM) and in the prostatic portion by hexamethonium (0.1 mM). The contractile response of the prostatic portion to exogenous acetylcholine was not inhibited by textrodotoxin (1.0 microM) but was attenuated by reserpine treatment (10 mg.kg-1 i.p. 24 h) and by prazosin or alpha, beta-methylene ATP. A combination of an alpha-1-adrenoceptor antagonist (prazosin) and P2 purinoceptor desensitization with alpha, beta-methylene ATP abolished the contractile response of the prostatic portion. The contraction induced by repetitive field stimulation of the prostatic portion was attenuated by hexamethonium whereas the response to a single stimulus was not modified. The data suggest that cholinomimetic drugs activate both nicotinic receptors located in nerve terminals of the prostatic portion and muscarinic receptors located in the smooth muscle cells of the epididymal portion, and that stimulation of nicotinic receptors induces the release of noradrenaline and ATP.
Carneiro
R C
RC
Department of Pharmacology, University of São Paulo, Brazil.
Markus
R P
RP
D000109:Acetylcholine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004822:Epididymis / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009638:Norepinephrine / Q000378:metabolism*; D011467:Prostate / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011978:Receptors, Nicotinic / Q000187:drug effects / Q000502:physiology*
D011978:Receptors, Nicotinic; D001285:Atropine; D000255:Adenosine Triphosphate; D000109:Acetylcholine; C002630:alpha,beta-methyleneadenosine 5'-triphosphate; D009638:Norepinephrine