Immunochemical localization and association with spermatozoa of androgen-regulated proteins of MR 24000 secreted by the mouse epididymis.
A polyclonal monospecific immune serum was raised against androgen-regulated proteins with Mr 24000 secreted by the mouse caput epididymidis. Sections of frozen tissues from the different regions of the epididymis have been studied by indirect immuno- fluorescence. Results indicate that the antigens are secretory proteins produced by the epithelial cells of the caput epididymidis, essentially in the medial and distal segments. Accumulation of the antigens was observed in the lumen of the caput and the corpus epididymal duct. Subsequently, their association with the sperm surface occurred and persisted down to the cauda epididymidis.
Laboratoire de Biologie cellulaire, Université Blaise Pascal, Aubiere, France.
C
C
Jimenez
N B
NB
Ghyselinck
A
A
Depeiges
J P
JP
Dufaure
D000818:Animals; D030501:Epididymal Secretory Proteins; D004822:Epididymis / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry; D008297:Male; D008667:Metalloproteins / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D013094:Spermatozoa / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D013735:Testicular Hormones / Q000276:immunology / Q000378:metabolism*
D030501:Epididymal Secretory Proteins; D007106:Immune Sera; D008667:Metalloproteins; D013735:Testicular Hormones