[Evaluation of the Mycoplasma Plus and the SIR Mycoplasma kits for quantitative detection and antibiotic susceptibility testing of genital mycoplasma].
We compared the results obtained with two commercially available systems (Diagnostics Pasteur) for the quantitative identification and the antibiotic susceptibility testing of the genital mycoplasmas. Ureaplasma urealyticum and Mycoplasma hominis with established methodologies, i.e. isolation on agar with enumeration by dilutions in broth medium and MIC determinations. The Mycoplasma Plus system, consisting of six cups, was designed for the identification and quantitation of genital mycoplasmas and the detection of yeasts. Used in parallel in 150 clinical specimens, it detected U. urealyticum in 42 out of 43 and M. hominis in 10 out of 11 specimens positive by the established methodology. The SIR Mycoplasma antibiogram, consisting of 16 cups, provided for the testing of 1 or 2 concentrations (micrograms/ml) of each of 8 antibiotics: doxycycline, minocycline and lymecycline (4-8); erythromycin (1-4); josamycin (2-8); clindamycin (2); pristinamycin (2); and ofloxacin (1-4). Using an inoculum of about 10(4)-10(5) organisms/ml, we found that major part of the results was in accord with those obtained with the MIC determined in broth for U. urealyticum and on agar for M. hominis. Strains intermediate or resistant to the tetracyclines were identified. Both systems seemed suitable for clinical laboratory use.
Laboratoire de Bactériologie, Hôpital Pellegrin, Deaux.
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Bebear
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001431:Bacteriological Techniques / Q000295:instrumentation*; D005260:Female; D005831:Genital Diseases, Female / Q000382:microbiology; D005832:Genital Diseases, Male / Q000382:microbiology; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008826:Microbial Sensitivity Tests; D009174:Mycoplasma / Q000187:drug effects / Q000302:isolation & purification*; D014509:Ureaplasma / Q000187:drug effects / Q000302:isolation & purification*; D015003:Yeasts / Q000302:isolation & purification
D000900:Anti-Bacterial Agents
Evaluation des systèmes Mycoplasma Plus et SIR Mycoplasma pour la détection quantitative et l'étude de la sensibilité aux antibiotiques des mycoplasmes génitaux.