Evolutionary and biosynthetic aspects of aspartate aminotransferase isoenzymes and other aminotransferases.
The mitochondrial and cytosolic isoenzymes of aspartate aminotransferase are homologous proteins. Both are encoded by nuclear DNA and synthesized on free polysomes. The organization of their genes is very similar, five out of a total of eight introns are located at the same nucleotide position. A variant consensus sequence was observed at the 3' splice site of introns of genes of imported mitochondrial proteins which may reflect the existence of splicing factors specific for the genes of this particular group of nuclear-encoded proteins. To date the amino acid sequences of 22 aminotransferases are known. A rigorous analysis yielded clear evidence that aspartate, tyrosine, and histidinol-phosphate aminotransferases are homologous proteins despite their low degree of sequence identity. The evolutionary relationship among the vitamin B6-dependent enzymes in general appears less clear. Conceivably, their common structural and mechanistic features are dictated by the chemical properties of pyridoxal 5'-phosphate rather than being due to a common ancestor of their protein moieties. In agreement with this notion, the ubiquitous active-site lysine residue that forms a Schiff base with the coenzyme can be replaced in the case of aspartate aminotransferase by a histidine residue without complete loss of catalytic competence.
Christen
P
P
Biochemisches Institut der Universität Zürich, Switzerland.
Jaussi
R
R
Juretić
N
N
Mehta
P K
PK
Hale
T I
TI
Ziak
M
M
D000818:Animals; D001219:Aspartate Aminotransferases / Q000096:biosynthesis* / Q000235:genetics; D005075:Biological Evolution; D003600:Cytosol / Q000201:enzymology; D007527:Isoenzymes / Q000096:biosynthesis* / Q000235:genetics; D008928:Mitochondria / Q000201:enzymology; D011736:Pyridoxine / Q000494:pharmacology; D000637:Transaminases / Q000096:biosynthesis*
D007527:Isoenzymes; D000637:Transaminases; D001219:Aspartate Aminotransferases; D011736:Pyridoxine