Antibody response of mouse splenocytes using mixture of supernatants of thymocytes, adherent and non-adherent splenocytes: in-vitro immunization-II.
We adapted one of the in-vitro immunization methods to induce antibody responses and confirmed the success of the immunization by enzyme-linked immunosorbent assay (ELISA) without hybridization. We have previously reported several methods of in-vitro immunization using different conditioned media. Here we introduce another method of in-vitro immunization using a mixture of three types of supernatant (thymocytes, and adherent and non-adherent splenocytes of mouse). Splenocytes were immunized in-vitro by a recombinant human growth hormone (rhGH) with the above conditioned media, and the results by ELISA showed a much higher optical density than the other in-vitro immunization methods that we had previously reported. Humoral responses as a result of in-vitro immunization to soluble antigens were easily confirmed by ELISA using the above-conditioned media. This finding indicates that any other conditions thought to be critical by other researches may not be essential for in-vitro immunization.
Kim
D
D
Department of Pediatrics and Clinical Pathology, Yonsei University College of Medicine, Seoul, Korea.
Noh
G W
GW
Kim
D H
DH
Kwon
O H
OH
D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology; D002448:Cell Adhesion; D005260:Female; D006133:Growth Substances / Q000276:immunology*; D007114:Immunization; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000941:Antigens; D006133:Growth Substances; D011994:Recombinant Proteins