Expression of macrophage colony-stimulating factor gene in the mouse brain during development.
We have investigated the expression of macrophage-colony stimulating factor (M-CSF) gene in mouse brain during development. Northern blot analysis of cerebral RNA evidenced a 4.5-kb M-CSF transcript from day 14 of gestation until 2 weeks after birth. The cell type responsible for this transcription was studied using in vitro cell cultures. The 4.5-kb M-CSF transcript was found both in astrocyte primary cultures and in immortalized astrocytic cell lines. M-CSF mRNA was also detected in lipopolysaccharide-stimulated brain macrophage cultures. These results suggest that M-CSF is involved in the outgrowth of microglia during ontogenesis.
Inserum U.114, Chaire de Neuropharmacologie, Collège de France, Paris.
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Théry
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Hétier
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Evrard
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Mallat
D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D003115:Colony-Stimulating Factors / Q000235:genetics* / Q000378:metabolism; D005314:Embryonic and Fetal Development; D005786:Gene Expression Regulation; D016173:Macrophage Colony-Stimulating Factor; D051379:Mice; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism
D003115:Colony-Stimulating Factors; D012333:RNA, Messenger; D016173:Macrophage Colony-Stimulating Factor