Cloning and characterization of the sisomicin-resistance gene from Micromonospora inyoensis.
We cloned DNA fragments of sisomicin-producing Micromonospora inyoensis into Streptomyces plasmid vectors and identified Streptomyces lividans TK24 transformants expressing the M. inyoensis sisomicin-resistance (sisA) gene. The sisA gene was compared to the previously reported Micromonospora purpurea Kan-Gen (kanamycin-gentamicin)-resistance gene. While the restriction endonuclease digestion patterns of the two determinants appear to be divergent, the genes are nonetheless closely related, based on similar patterns and levels of aminoglycoside-resistance and their ability to cross-hybridize under stringent conditions. We have transformed recombinant plasmid pMD5-2, which carries the sisA gene, into our M. purpurea gentamicin-production strain and determined that gentamicin biosynthesis was not improved.
Department of Microbiological and Cell Culture Development, Schering Corporation, Union, NJ 07083.
S L
SL
Goldberg
J G
JG
Romero
Y M
YM
Deo
D015139:Blotting, Southern; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis*; D004352:Drug Resistance, Microbial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005839:Gentamicins / Q000096:biosynthesis; D008847:Micromonospora / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D015394:Molecular Structure; D016296:Mutagenesis; D010957:Plasmids; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D012853:Sisomicin / Q000494:pharmacology*; D013302:Streptomyces / Q000235:genetics; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D005839:Gentamicins; D012853:Sisomicin