Endothelin-3 concentrations in human plasma: the increased concentrations in patients undergoing haemodialysis.
Plasma immunoreactive endothelin-3 (ir-ET-3) concentrations were measured by a sandwich-enzyme immunoassay (sandwich-EIA) for endothelin-3 (ET-3). The assay method consists of two antibodies directed against N-terminal and C-terminal portions of ET-3. It detects as little as 0.1 pg/well of ET-3 without the crossreaction with endothelin-1, endothelin-2 and big ET-3. Plasma ir-ET-3 concentrations were found to be 0.45 +/- 0.07 pg/ml (mean +/- SD) in healthy volunteers, and were increased in patients undergoing haemodialysis (0.83 +/- 0.26 pg/ml, p less than 0.001). In reverse-phase HPLC, ir-ET-3 in normal plasma and in plasma of haemodialysis patients was eluted at the position of authentic ET-3, indicating that ir-ET-3 in plasma detected by the EIA was ET-3 itself. These results suggest that circulating ET-3 exists in normal human plasma and that production and/or metabolism of ET-3 may be altered in patients undergoing haemodialysis.
Tsukuba Research Laboratories, Takeda Chemical Industries, Ltd., Ibaraki, Japan.
N
N
Suzuki
H
H
Matsumoto
T
T
Miyauchi
K
K
Goto
T
T
Masaki
M
M
Tsuda
M
M
Fujino
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D016232:Endothelins; D004730:Endothelium, Vascular; D006801:Humans; D007124:Immunoenzyme Techniques; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000628:therapy; D008297:Male; D010455:Peptides / Q000097:blood*; D012016:Reference Values; D006435:Renal Dialysis
D016232:Endothelins; D010455:Peptides