Bovine recombinant granulocyte-macrophage colony-stimulating factor enhancement of bovine neutrophil functions in vitro.
Neutrophils were purified from blood of dexamethasone-treated (0.04 mg/kg of body weight) and untreated calves. Cells were untreated (controls) or cultured in media containing 5 or 10 ng of bovine recombinant granulocyte-macrophage colony-stimulating factor (rbGM-CSF)/ml for 10 to 12 hours before being tested for various functions. Dexamethasone treatment of calves decreased luminol-dependent chemiluminescence, decreased phagocytosis of Pasteurella multocida and several Staphylococcus spp by various degrees, and decreased antibody-dependent cell-mediated cytotoxicity against bovine herpesvirus-infected cells by 26 to 32%. The percentage phagocytosis of coagulase-positive S aureus and S intermedius was higher than that of coagulase-negative S epidermidis for neutrophils from all calves. Culture of neutrophils with rbGM-CSF significantly increased (P less than 0.05) all of the aforementioned functions, compared with control neutrophils; however, rbGM-CSF-induced increases in function tended to be higher in neutrophils from dexamethasone-treated calves than in neutrophils from untreated calves.
Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan 66506.
P G
PG
Reddy
D S
DS
McVey
M M
MM
Chengappa
F
F
Blecha
H C
HC
Minocha
P E
PE
Baker
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity / Q000276:immunology; D002417:Cattle; D003115:Colony-Stimulating Factors / Q000097:blood / Q000494:pharmacology*; D003907:Dexamethasone / Q000008:administration & dosage / Q000494:pharmacology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000097:blood / Q000494:pharmacology*; D007273:Injections, Intramuscular / Q000662:veterinary; D008163:Luminescent Measurements; D008165:Luminol; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D010325:Pasteurella / Q000187:drug effects / Q000502:physiology; D010587:Phagocytosis / Q000187:drug effects; D011897:Random Allocation; D011994:Recombinant Proteins / Q000097:blood / Q000494:pharmacology; D013210:Staphylococcus / Q000187:drug effects / Q000502:physiology; D013997:Time Factors
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D011994:Recombinant Proteins; D008165:Luminol; D003907:Dexamethasone; D016178:Granulocyte-Macrophage Colony-Stimulating Factor