[Diagnosis of HIV by clinical pathological methods].
HIV (human immunodeficiency virus)-I infection can be detected by the presence of HIV antigen, antibody to HIV, or gene of HIV itself in patient's blood. There are a considerable number of such antibody detecting methods (first generation) commercially available, but even the first was introduced in only mid-1985. These tests detect antibody in more than 90% of patients with confirmed AIDS. Recently, new antibody detecting methods (second generation) was produced using a single antigen (e.g. p 24, gp 41) of HIV-I by recombinant DNA or synthetic peptide. Furthermore, detection methods for DNA or RNA of HIV were developed and these methods are introduced in clinical use in near future.
Sakurabayashi
I
I
Department of Clinical Laboratory, Omiya Medical Center Jichi Medical School.
Matsuda
J
J
D015492:AIDS Serodiagnosis; D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis*; D015153:Blotting, Western; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D006678:HIV / Q000235:genetics / Q000276:immunology; D015483:HIV Antibodies / Q000032:analysis; D015488:HIV Antigens / Q000032:analysis; D006801:Humans; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests
D004279:DNA, Viral; D015483:HIV Antibodies; D015488:HIV Antigens