A probe for the detection of methicillin-resistant Staphylococcus aureus.
An approximately 300 base pair DNA fragment for use as a probe was isolated from methicillin-resistant Staphylococcus aureus DNA partially digested with Sau3AI. This probe hybridized with 25 methicillin-resistant clinical isolates of Staphylococcus aureus belonging to 18 different phage types, but not with 41 clinical isolates susceptible to methicillin.
Laboratory for Medical Microbiology, University Hospital Utrecht, The Netherlands.
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D016172:DNA Fingerprinting; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics; D009693:Nucleic Acid Hybridization; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*
D015342:DNA Probes; D004269:DNA, Bacterial