Immune response of rabbits to native G-actins.
Actins are highly conserved proteins and are therefore claimed to be not very immunogenic without prior denaturation or chemical modification. We have obtained in rabbits high-titered antibodies to "native" G-actins from chicken and man, and assayed their cross-reaction using an enzyme immunoassay, Western blotting and immunohistochemistry. The antigens differ in their ability to induce antibody formation (chicken gizzard actin [(beta), gamma] greater than chicken skeletal actin [alpha] = human platelet actin [beta, (gamma)]). Antibodies to skeletal actin [alpha] are muscle-specific and mainly directed against the homologous region comprising the N-terminus (residues 1-226). Antibodies to gizzard actin [(beta), gamma] cross-react, to a lesser extent, with the alpha and beta, (gamma) isoforms. They show no regional specificity within the homologous antigen. Antibodies to the tryptic core fragment (residues 69-374) of skeletal actin react with fragments comprising the C-terminal part of muscular actins. Antibodies to platelet actin [beta, (gamma)] cross-react with muscular actins, recognizing not the native, but slightly degraded molecules. Platelet actin induces the formation of high-titered albumin antibodies for hitherto unknown reasons.
Institut für Zoologie, Technische Hochschule, Darmstadt, F.R.G.
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Stuewer
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Gröschel-Stewart
D000199:Actins / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology*; D001792:Blood Platelets / Q000032:analysis; D015153:Blotting, Western; D002645:Chickens; D005455:Fluorescent Antibody Technique; D005895:Gizzard, Avian / Q000032:analysis; D007114:Immunization; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007525:Isoelectric Focusing; D009132:Muscles / Q000032:analysis; D011817:Rabbits
D000199:Actins; D000906:Antibodies; D000941:Antigens