The autoantigen Ku is indistinguishable from NF IV, a protein forming multimeric protein-DNA complexes.
We have isolated a cDNA encoding the 84-kD subunit of NFIV. Tryptic peptide sequences were identified within the coding sequences, confirming its proper identity. The primary sequence of the protein is identical to that of the large subunit of the Ku autoantigen. A missing NFIV peptide sequence was identified within the sequence of the small subunit of Ku. In addition, the proteins are identical in immunological aspects. We suggest that the Ku and NFIV proteins are identical. This connection adds new biochemical data to our knowledge of the Ku autoantigen.
Laboratory for Physiological Chemistry, University of Utrecht, The Netherlands.
M H
MH
Stuiver
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Coenjaerts
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PC
van der Vliet
D000595:Amino Acid Sequence; D000818:Animals; D034961:Antigens, Nuclear; D000954:Antigens, Surface / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D004247:DNA / Q000032:analysis; D004265:DNA Helicases; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D000072200:Ku Autoantigen; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D010449:Peptide Mapping; D011233:Precipitin Tests; D011817:Rabbits
D034961:Antigens, Nuclear; D000954:Antigens, Surface; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D004247:DNA; D004265:DNA Helicases; C106893:XRCC5 protein, human; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen