Soybean phosphatidylcholine vesicles containing plant sterols: a fluorescence anisotropy study.
The typical plant sterols (sitosterol, stigmasterol and campesterol) were compared with respect to their ability to regulate membrane fluidity of soybean phosphatidylcholine (PC) vesicles. Fluidity changes were monitored by the steady-state fluorescence anisotropy with 1,6-diphenyl-1,3,5-hexatriene as a probe and assigned to a measure of the acyl chain orientational order. Sitosterol and campesterol appear to be the most suitable sterols in ordering the acyl chains of soybean lecithin bilayers, even more efficient than cholesterol, the standard of reference for sterol effects on membranes, suggesting that they play a significant role in the regulation of plant membrane properties. Stigmasterol is shown to be much less active. Cycloartenol, a biosynthetic precursor of plant sterols, increases the acyl chain order with the same efficiency as cholesterol. We also investigated the effects of two unusual sterols, 24-methylpollinastanol and 14 alpha,24-dimethylcholest-8-en-3 beta-ol, which were shown to accumulate in plants treated with fungicides belonging to two important classes, N-substituted morpholines and triazoles, respectively. These two sterols exhibit a behavior very similar to that of stigmasterol. The results are discussed in terms of sterol effects on the molecular packing of soybean PC bilayers.
Schuler
I
I
Départment d'Enzymologie Moléculaire et Cellulaire de l'Institute de Biologie Moléculaire des Plantes (UPR CNRS 406), Strasbourg, France.
Duportail
G
G
Glasser
N
N
Benveniste
P
P
Hartmann
M A
MA
D002784:Cholesterol / Q000031:analogs & derivatives / Q000494:pharmacology; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D008051:Lipid Bilayers / Q000378:metabolism*; D008081:Liposomes; D008560:Membrane Fluidity / Q000187:drug effects*; D010713:Phosphatidylcholines; D010840:Phytosterols / Q000494:pharmacology*; D012855:Sitosterols / Q000494:pharmacology; D012995:Solubility; D013025:Glycine max; D013265:Stigmasterol / Q000494:pharmacology
D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; D010840:Phytosterols; D012855:Sitosterols; C021273:campesterol; D002784:Cholesterol; D013265:Stigmasterol