In situ enzyme-linked immunosorbent assay to quantitate in vitro development of Eimeria tenella.
An in situ enzyme-linked immunosorbent assay was developed to measure in vitro development of Eimeria tenella. The assay used a polyclonal, anti-merozoite serum produced by immunization with culture-derived, chromatographically purified merozoites. Although this antiserum cross-reacted with sporozoite-infected cultures (by indirect immunofluorescence and in situ enzyme-linked immunosorbent assay), it clearly distinguished the increase in antigen synthesized throughout intracellular growth. The assay can be used for high-throughput, anticoccidial drug screening, for which it gives quantitative results that are comparable to the published radiometric [( 3H]uracil incorporation) endpoint.
Pfizer Central Research, Groton, Connecticut 06340.
J A
JA
Olson
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis; D002478:Cells, Cultured; D003429:Cross Reactions; D004539:Eimeria / Q000187:drug effects / Q000254:growth & development* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D008854:Microscopy, Electron; D014498:Uracil / Q000378:metabolism
D000953:Antigens, Protozoan; D014498:Uracil