A knowledge-based experimental design system for nucleic acid engineering.
Presented in this paper is a knowledge-based experimental design system that incorporates the domain expertise used in nucleic acid engineering, thus automating the processing of error-prone, laborious low-level work, and many decision-making steps, and guiding the biologist toward a workable plan. This allows the biologist to work at a higher abstraction level, concentrating on more fundamental, difficult and challenging problems directly related to protein structure - function relationships. Cassette-based site-directed mutagenesis and synthetic gene designs are used as examples to illustrate the utility of the knowledge-based system approach to experimental design.
Jiang
K
K
Department of Biomedical Engineering, Vanderbilt University, Nashville, TN 37232.
Zheng
J
J
Higgins
S B
SB
Watterson
D M
DM
Craig
T A
TA
Lukas
T J
TJ
Van Eldik
L J
LJ
D000465:Algorithms; D000595:Amino Acid Sequence; D001185:Artificial Intelligence; D001483:Base Sequence; D004252:DNA Mutational Analysis; D008838:Microcomputers; D008969:Molecular Sequence Data; D015202:Protein Engineering / Q000379:methods*; D015183:Restriction Mapping; D009418:S100 Proteins / Q000235:genetics; D012984:Software; D014584:User-Computer Interface
D009418:S100 Proteins