PCR amplification of an Escherichia coli gene using mixed primers containing deoxyinosine at ambiguous positions in degenerate amino acid codons.
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Department of Biological Chemistry, University of Michigan, Ann Arbor 48109-0606.
Dekker
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D003062:Codon; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D007288:Inosine / Q000031:analogs & derivatives*; D021141:Nucleic Acid Amplification Techniques; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D007652:Oxo-Acid-Lyases / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013698:Templates, Genetic
D003062:Codon; D009838:Oligodeoxyribonucleotides; D007288:Inosine; D007652:Oxo-Acid-Lyases; C021415:4-hydroxy-2-oxoglutarate aldolase; C012271:deoxyinosine