Ethanol fermentation by nystatin-resistant strains of Saccharomyces cerevisiae.
Nystatin-resistant mutants of haploid and polyploid strains of Saccharomyces cerevisiae were isolated by plating on gradient plates with increasing nystatin concentrations (60-3000 U/ml). Some of the mutants were defective in ergosterol biosynthesis, and produced zymosterol and cholestatetraenol-like sterols. Those mutants which do not form ergosterol produce less ethanol than the parent strains. They also had lower viability during fermentation of glucose solutions (8-13% vs. 33-47%). This became more pronounced in fermentations of higher concentrations of glucose. A nystatin-resistant but ergosterol-forming mutant had a similar fermentation capacity to the parent strain.
Grunwald-Raij
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H
Department of Food Engineering and Biotechnology, Technion, Israel Institute of Technology, Technion City, Haifa.
Margalith
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P
D002855:Chromatography, Thin Layer; D004352:Drug Resistance, Microbial; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D015394:Molecular Structure; D009761:Nystatin / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D013261:Sterols / Q000032:analysis
D013261:Sterols; D009761:Nystatin; D000431:Ethanol; D005947:Glucose