A cell culture, chromosomal and quantitative DNA analysis of a metastatic epithelioid sarcoma. Deletion 1p, a possible primary chromosomal abnormality in epithelioid sarcoma.
The chromosomal banding pattern and the in vitro growth characteristics of a metastatic epithelioid sarcoma are described. The cultured tumor cells retained growth characteristics as well as ultrastructural and immunohistochemical properties similar to the cells of the primary tumor. Cytogenetic analysis revealed a modal range in the diploid-hypodiploid region, a finding which was corroborated by quantitative DNA determinations of both the primary tumor and a lymph node metastasis. Fourteen different marker chromosomes were identified. The most frequent clonal rearrangement was a 1p-marker resulting from a short arm terminal deletion, i.e., del (1) (p21-22). A similar 1p- marker has previously been observed in an established epithelioid sarcoma cell line. The finding of an apparently identical 1p-marker in two of two analyzed epithelioid sarcomas suggests that this rearrangement may be a primary cytogenetic abnormality in epithelioid sarcoma. An elevated ras p21 expression was demonstrated using immunohistochemical methods. The possible involvement of the N-ras gene and/or a tumor suppressor in the 1p deletion is considered.
Department of Oral Pathology, Gothenburg University, Sweden.
G
G
Stenman
L G
LG
Kindblom
J
J
Willems
L
L
Angervall
D002869:Chromosome Aberrations / Q000235:genetics*; D002871:Chromosome Banding; D002872:Chromosome Deletion; D025063:Chromosome Disorders; D004273:DNA, Neoplasm / Q000032:analysis*; D005542:Forearm; D006801:Humans; D007621:Karyotyping; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D015689:Oncogene Protein p21(ras) / Q000032:analysis; D012509:Sarcoma / Q000235:genetics* / Q000556:secondary / Q000648:ultrastructure; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D015689:Oncogene Protein p21(ras)