Preparative high-performance liquid chromatography on chemically modified porous glass. Isolation of saponins from ginseng.
Preparative high-performance liquid chromatography on octadecylsilyl porous glass (pore size, 550 A) with acetonitrile-water as the mobile phase was applied for the isolation of saponins from Panax ginseng. In a single run, several milligrams of pure ginsenosides were obtained from 10 g of roots of Panax ginseng. The method was simple, rapid and convenient and should be applicable to isolation of other saponins of crude drugs.
Kanazawa
H
H
Kyoritsu College of Pharmacy, Tokyo, Japan.
Nagata
Y
Y
Matsushima
Y
Y
Tomoda
M
M
Takai
N
N
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D005894:Panax / Q000032:analysis*; D010946:Plants, Medicinal; D012503:Saponins / Q000302:isolation & purification*
D012503:Saponins