Antisense RNA of the latent period gene (MER5) inhibits the differentiation of murine erythroleukemia cells.
The MER5 cDNA was cloned from RNA preferentially synthesized in murine erythroleukemia (MEL) cells during the early period of MEL cell differentiation. To understand the role of the MER5 gene in the differentiation, we have transferred the MER5 cDNA into MEL cells in both sense and antisense orientations under control of the promoter of the human metallothionein gene. Only in the transformants with the antisense MER5 cDNA, did their elevated expression inhibit differentiation. The result suggests that the MER5 gene product may promote early events in the differentiation of MEL cells.
Department of Cell Biology, Tohoku University, Sendai, Japan.
Y
Y
Nemoto
T
T
Yamamoto
S
S
Takada
Y
Y
Matsui
M
M
Obinata
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation; D002712:Chlorides / Q000494:pharmacology; D003001:Cloning, Molecular; D004912:Erythrocytes / Q000166:cytology*; D005796:Genes; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute; D008668:Metallothionein / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D016372:RNA, Antisense / Q000235:genetics*; D014162:Transfection; D014170:Transformation, Genetic; D014407:Tumor Cells, Cultured; D015032:Zinc / Q000494:pharmacology; D017967:Zinc Compounds
D002712:Chlorides; D016372:RNA, Antisense; D017967:Zinc Compounds; C016837:zinc chloride; D008668:Metallothionein; D015032:Zinc