Production of granulocyte/macrophage colony-stimulating factor by cultured astrocytes.
We investigated the production of interleukin-3 (IL-3)-like factor by murine astrocytes. Supernatants from lipopolysaccharide (LPS)-stimulated astrocytes induced proliferation of IC-2, an IL-3- and granulocyte/macrophage colony-stimulating factor (GM-CSF)-dependent cell line. This activity was completely neutralized by the antibody against GM-CSF but not by the anti-IL-3 monoclonal antibody. Northern blot analysis revealed the expression of GM-CSF mRNA, but not of IL-3 mRNA, in cultured astrocytes. These results indicate that with proper stimuli murine astrocytes produce GM-CSF.
Division of Cell Biology, School of Medicine, Fujita Health University, Aichi, Japan.
K
K
Ohno
A
A
Suzumura
M
M
Sawada
T
T
Marunouchi
D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies; D000911:Antibodies, Monoclonal; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D015152:Blotting, Northern; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D015870:Gene Expression; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D007377:Interleukin-3 / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification
D000906:Antibodies; D000911:Antibodies, Monoclonal; D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007377:Interleukin-3; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D016178:Granulocyte-Macrophage Colony-Stimulating Factor