The high resolution G- and R-banding pattern in chromosomes of river buffalo (Bubalus bubalis L.).
High resolution G- and R-banding patterns in chromosomes of river buffalo (Bubalus bubalis L.) were obtained by using early (G-bands) and late (R-bands) BrdU-incorporation in synchronized cell cultures. To better characterize the river buffalo chromosomes, GTG-, GBG-, and RBG-techniques were used. The total number of bands achieved were 490 (207 G-positive, 207 R-positive, 45 variable, and 31 centromeric regions). Only one common G- and R-banding nomenclature was reported. The number, position and intensity of G bands were highly similar by the structural GTG and the replicating GBG-techniques. However, the replicating G- and R-bands appeared to be more distinct and reproducible than the structural G-bands. Some changes in chromosome nomenclature (chromosomes 1p, 2p, 5p, and 21) were made when referred to the cattle homologues.
Consiglio Nazionale delle Ricerche, Istituto di ricerche sull'Adattamento dei Bovini e dei Bufali all' Ambiente del Mezzogiorno (CNR-IABBAM), Napoli, Italia.
L
L
Iannuzzi
G P
GP
Di Meo
A
A
Perucatti
L
L
Ferrara
D000818:Animals; D002020:Buffaloes / Q000235:genetics*; D002478:Cells, Cultured; D002871:Chromosome Banding / Q000379:methods; D008297:Male; D009626:Terminology as Topic