1,25-Dihydroxyvitamin D3 suppresses dexamethasone effects on calcitonin secretion.
1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) and dexamethasone (DEX) influence synthesis and secretion of various hormones. Recent reports concerning the interaction of the two steroids revealed opposite--agonistic as well as antagonistic--effects in different biological systems. As calcitonin (CT) gene expression is affected by both agents, inhibited by 1,25(OH)2D3 and stimulated by DEX, we utilized CT secretion and storage as a model to study the combined effects of the two hormones. A human C cell carcinoma cell line (TT) was used, incubating the cells for a period of 4 days with 1,25(OH)2D3 and DEX alone and in combination. 1,25(OH)2D3 resulted in a decrease, whereas DEX resulted in a increase of CT secretion and content. Combining the two steroids, 1,25(OH)2D3 surprisingly abolished the stimulation of DEX on CT secretion and content. The underlying mechanism is yet unclear and could be envisioned to include steroid receptor regulation or gene transcription.
Department of Internal Medicine I, University of Heidelberg, F.R.G.
M
M
Lazaretti-Castro
A
A
Grauer
F
F
Raue
R
R
Ziegler
D000818:Animals; D002116:Calcitonin / Q000378:metabolism*; D002117:Calcitriol / Q000494:pharmacology*; D002460:Cell Line; D003907:Dexamethasone / Q000037:antagonists & inhibitors*; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D012636:Secretory Rate / Q000187:drug effects
D003907:Dexamethasone; D002116:Calcitonin; D002117:Calcitriol