Penetration of synthetic corticosteroids into human aqueous humour.
The penetration of prednisolone acetate (1%) and fluorometholone alcohol (0.1%) into human aqueous humour following topical application was determined using the very sensitive and specific technique of Gas Chromatography with Mass Spectrometry (GCMS). Prednisolone acetate afforded peak mean concentrations of 669.9 ng/ml within two hours and levels of 28.6 ng/ml in aqueous humour were detected almost 24 hours post application. The peak aqueous humour level of fluorometholone was 5.1 ng/ml. The results are compared and contrasted with the absorption of dexamethasone alcohol (0.1%), betamethasone sodium phosphate (0.1%) and prednisolone sodium phosphate (0.5%) into human aqueous humour.
Department of Ophthalmology, Southern General Hospital, Glasgow.
C N
CN
McGhee
D G
DG
Watson
J M
JM
Midgley
M J
MJ
Noble
G N
GN
Dutton
A I
AI
Fern
D000368:Aged; D000369:Aged, 80 and over; D001082:Aqueous Humor / Q000378:metabolism*; D005469:Fluorometholone / Q000493:pharmacokinetics*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008875:Middle Aged; D009883:Ophthalmic Solutions; D011239:Prednisolone / Q000031:analogs & derivatives* / Q000493:pharmacokinetics
D009883:Ophthalmic Solutions; C009935:prednisolone acetate; D011239:Prednisolone; D005469:Fluorometholone