Four genes for the calpain family locate on four distinct human chromosomes.
Calcium dependent proteases (calpains, CAPNs, E.C.3.4.22.17) constitute a family of proteins which share a homologous cysteine-protease domain (large subunits, L1, L2, and L3) and an E-F hand Ca2(+)-binding domain (L1, L2, L3, and small subunit, S). We have mapped the genes for four calpain proteins (L1, L2, L3, and S) on four distinct human chromosomes by a combination of spot-blot hybridization to flow-sorted chromosomes and Southern hybridization of DNAs from a human x mouse hybrid cell panel. The genes for calpain L1 (CAPN1, large subunit of calpain I), L2 (CAPN2, large subunit of calpain II), L3 (CAPN3, a protein related to the large subunits), and S (CAPN4, a small subunit common to calpains I and II) were assigned to human chromosomes 11, 1, 15, and 19, respectively.
Department of Molecular Biology, Tokyo Metropolitan Institute of Medical Science, Japan.
S
S
Ohno
S
S
Minoshima
J
J
Kudoh
R
R
Fukuyama
Y
Y
Shimizu
S
S
Ohmi-Imajoh
N
N
Shimizu
K
K
Suzuki
D002154:Calpain / Q000235:genetics*; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D002880:Chromosomes, Human, Pair 11; D002884:Chromosomes, Human, Pair 15; D002888:Chromosomes, Human, Pair 19; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D006801:Humans; D006822:Hybrid Cells; D015183:Restriction Mapping
D015342:DNA Probes; D004247:DNA; D002154:Calpain