Transcriptional control of SSL1, a gene controlling alpha-specific inactivation of a-factor in Saccharomyces cerevisiae.
The SSL1 gene of S. cerevisiae which is involved in the cell-type specific recovery of mating type alpha cells from cell cycle arrest by the mating hormone a-factor has been cloned as a 1 kb Sau3A fragment in a complementing plasmid from a library of S. cerevisae genomic DNA. Disruption of this gene in wild-type alpha-cells results in the ssl1- phenotype which is characterized by an extreme sensitivity to a-factor. Transcription analysis revealed that the SSL1 gene is transcribed only in MAT alpha cells but not in MAT alpha or diploid (MAT alpha/MAT alpha) cells defining it as a member of the set of alpha-specific genes.
Steden
M
M
Institut für Physiologische Chemie, Ruhr-Universität Bochum, F.R.G.
Duntze
W
W
D015139:Blotting, Southern; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D005809:Genes, Regulator; D000072235:Mating Factor; D010455:Peptides / Q000235:genetics*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic
D010455:Peptides; D000072235:Mating Factor