Colorimetric liposome lysis for assay of anti-streptolysin O antibody.
We developed an automated colorimetric assay based on liposome lysis for measurement of the titer of anti-streptolysin O antibodies (ASO) in human sera by using liposomes in which alkaline phosphatase (EC 3.1.3.1) was entrapped. The assay involved the inhibition by ASO of liposome lysis caused by streptolysin O. The procedure was fully automated for use with a routine clinical automated analyzer. The inter- and intra-assay precision showed that results were reproducible. Potential interfering substances such as bilirubin, hemoglobin, and triglycerides did not affect the results. With this assay, results correlated well with those by the Rantz-Randall method and the latex agglutination method (r = 0.900 and 0.890, respectively). This automated colorimetric assay for ASO should be of use for diagnosing streptococcal infections.
Kubotsu
K
K
Osaka Research Division, Wako Pure Chemical Industries, Ltd., Japan.
Ushio
I
I
Yoshikawa
K
K
Kida
M
M
Ishikawa
K
K
Matsuura
S
S
Sakurabayashi
I
I
D000469:Alkaline Phosphatase / Q000097:blood; D000906:Antibodies / Q000032:analysis; D001322:Autoanalysis; D001426:Bacterial Proteins; D003124:Colorimetry; D008081:Liposomes / Q000737:chemistry* / Q000276:immunology; D013301:Streptolysins / Q000097:blood* / Q000276:immunology
D000906:Antibodies; D001426:Bacterial Proteins; D008081:Liposomes; D013301:Streptolysins; C025964:streptolysin O; D000469:Alkaline Phosphatase