Reactive oxygen species and hyaluronate in serum and synovial fluid in arthritis.
Oxygen-derived free radicals (ODFR) depolymerized synovial-fluid (SF) hyaluronic acid (HA) when hypoxanthine/xanthine oxidase (HX/XAO) was used as the radical generator. The molecular-weight distribution of ODFR-induced SF HA degradation products was determined using high performance liquid chromatography (HPLC) with TSK 5000 PW or TSK 6000 PW size-exclusion columns and simultaneously using 125I-labelled hyaluronate-binding protein (125I-HABP) assay. The exposure of SF HA to hydroxyl-radical flux resulted in the formation of a degradation product having a molecular weight of 13.5 X 10(3) daltons, from which no further degradation was achieved. If the iron chelator desferrioxamine and hydroxyl-radical scavenger mannitol were present in the reaction mixture, the HA peak decreased by 30-50%, as a result of reaction with superoxide radical and hydrogen peroxide. These results show that superoxide radical and hydroxyl radical may have different modes of action on SF HA. The molecular-weight distribution of serum HA from patients with rheumatoid arthritis varied in different individuals and ranged between 275 X 10(3) and 650 X 10(3).
Department of Medicine, Helsinki University Central Hospital, Finland.
H
H
Saari
T
T
Sorsa
Y T
YT
Konttinen
D001172:Arthritis, Rheumatoid / Q000097:blood / Q000378:metabolism*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D005609:Free Radicals; D006801:Humans; D006820:Hyaluronic Acid / Q000097:blood / Q000378:metabolism*; D010100:Oxygen / Q000097:blood / Q000378:metabolism*; D011874:Radiometry; D013582:Synovial Fluid / Q000378:metabolism*
D005609:Free Radicals; D006820:Hyaluronic Acid; D010100:Oxygen