The enhancing effect of ethanol on the development of glutatione S-transferase placental form-positive foci induced by diethylnitrosamine in F344 rat.
The effects of ethyl alcohol and pig serum administration on the development of preneoplastic hepatic enzyme-altered foci were examined in an in vivo mid-term assay system. Rats were initially given a single dose (200 mg/Kg) intraperitoneal injection of diethylnitrosamine (DEN). Two weeks later, treatment was started with 10% ethanol + 10% sucrose solution, 10% sucrose solution, or tap water as drinking water for 6 weeks with or without intraperitoneal injection of porcine serum twice a week. All rats were subjected to a two-thirds partial hepatectomy at week 3. The modification potentials were evaluated by comparing the number and area per cm2 of glutathione S-transferase placental form-positive (GST-P+) foci in the liver of each group. As a result, ethanol significantly enhanced the development of GST-P+ foci. Unfortunately, the porcine serum injection produced no hepatic fibrosis and no significant alteration in GST-P+ foci.
Department of Anatomical Pathology, Korea Cancer Center Hospital.
K J
KJ
Cho
N H
NH
Myong
J J
JJ
Jang
D000818:Animals; D004052:Diethylnitrosamine / Q000633:toxicity*; D000431:Ethanol / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000378:metabolism*; D007106:Immune Sera / Q000494:pharmacology; D008104:Liver Cirrhosis, Alcoholic / Q000201:enzymology; D008297:Male; D010920:Placenta / Q000187:drug effects / Q000201:enzymology*; D011230:Precancerous Conditions / Q000139:chemically induced* / Q000201:enzymology; D051381:Rats; D011916:Rats, Inbred F344; D015996:Survival Rate; D013552:Swine
D007106:Immune Sera; D004052:Diethylnitrosamine; D000431:Ethanol; D005982:Glutathione Transferase