Direct demonstration of the lectin activity of gp90MEL, a lymphocyte homing receptor.
Considerable evidence implicates gp90MEL as a lymphocyte homing receptor mediating lymphocyte attachment to high endothelial venules of lymph nodes in mouse. The protein appears to function as a calcium-dependent, lectin-like receptor as inferred primarily by the ability of specific carbohydrates to block its function and by the presence of a calcium-type lectin domain in its primary sequence. An ELISA assay is described which provides the first demonstration that the isolated protein has lectin activity and allows a further definition of its carbohydrate specificity. In addition to the monosaccharides mannose-6-phosphate and fructose-1-phosphate, ligand activity is shown for the sulfated glycolipid, sulfatide, and for two sulfated fucose-containing polysaccharides (fucoidin and egg jelly coat) from nonmammalian sources.
Department of Anatomy, University of California, San Francisco 94143-0452.
Y
Y
Imai
D D
DD
True
M S
MS
Singer
S D
SD
Rosen
D000818:Animals; D001667:Binding, Competitive; D002118:Calcium / Q000502:physiology; D002448:Cell Adhesion / Q000502:physiology; D004729:Endothelium, Lymphatic / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006017:Glycolipids / Q000378:metabolism; D037102:Lectins; D008214:Lymphocytes / Q000378:metabolism; D008351:Mannans / Q000378:metabolism; D008360:Mannosephosphates / Q000378:metabolism; D051379:Mice; D009005:Monosaccharides / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D011971:Receptors, Immunologic / Q000502:physiology*; D016201:Receptors, Lymphocyte Homing; D013433:Sulfoglycosphingolipids / Q000378:metabolism
D006017:Glycolipids; D037102:Lectins; D008351:Mannans; D008360:Mannosephosphates; D009005:Monosaccharides; D011134:Polysaccharides; D011971:Receptors, Immunologic; D016201:Receptors, Lymphocyte Homing; D013433:Sulfoglycosphingolipids; C065532:mannose-6-phosphate rich phosphomannan; C007789:fucoidan; D002118:Calcium