Construction of a bivalent oral vaccine for prevention of typhoid fever and cholera diarrhea.
A recombinant plasmid pMM-CTB containing the gene for production of the nontoxic B subunit of Vibrio cholera was transferred into a safe, effective and attenuated oral vaccine Ty21a strain of Salmonella typhi. The resulting Ty21a (pMM-CTB) could steadily produce CT-B subunit that was secreted extracellularly and had the same antigenicity as CT-B produced by V. cholera. Furthermore, the characteristics of the antigenicity, the persistance in mice and the galactose sensitivity possessed in the strain of Ty21a were also retained in Ty21a (pMM-CTB). A bivalent vaccine containing Ty21a (pMM-CTB) and the killed whole cell of V. cholera was then constructed which had good immunogenecity for typhoid fever and cholera diarrhea.
Institute of Biotechnology, Academy of Military Medical Sciences, Beijing.
Q J
QJ
Ma
X Q
XQ
Yu
C X
CX
Liu
J G
JG
Zhou
L S
LS
Xiong
C F
CF
Huang
D000818:Animals; D022121:Cholera Vaccines / Q000302:isolation & purification; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D005818:Genetic Engineering; D006168:Guinea Pigs; D051379:Mice; D010957:Plasmids; D011817:Rabbits; D014436:Typhoid-Paratyphoid Vaccines; D014612:Vaccines / Q000302:isolation & purification*; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic / Q000302:isolation & purification*
D022121:Cholera Vaccines; D004274:DNA, Recombinant; D014436:Typhoid-Paratyphoid Vaccines; D014612:Vaccines; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic