High-level expression of the FtsA protein inhibits cell septation in Escherichia coli K-12.
DNA fragments encoding the ftsA gene were subcloned into plasmids downstream of a lac promoter or a tac promoter. These plasmid constructs, when transformed into wild-type and mutant strains, inhibited normal cell septation, causing the formation of long nonseptate filaments. This phenotype is due to overproduction of the FtsA protein.
Department of Microbiology, Louisiana State University, Baton Rouge.
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Gayda
D001426:Bacterial Proteins / Q000235:genetics*; D002455:Cell Division; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects / Q000235:genetics*; D029968:Escherichia coli Proteins; D015870:Gene Expression; D005798:Genes, Bacterial; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D009154:Mutation; D010957:Plasmids; D015183:Restriction Mapping
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C065103:FtsA protein, Bacteria; C489310:FtsA protein, E coli; D007544:Isopropyl Thiogalactoside