Mitogen response and cell cycle kinetics of Swiss 3T3 cells in defined medium: differences from human fibroblasts and effects of cell density.
The mitogen requirement and proliferative response of Swiss 3T3 cells in serum-free, chemically defined culture medium were compared with those of early-passage human diploid fibroblasts. The effects of platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin, transferrin, and dexamethasone on cell-cycle parameters were measured using 5'-bromo-deoxyuridine-Hoechst flow cytometry. Swiss 3T3 cells differ from human fibroblasts in several ways: (1) Swiss 3T3 cells showed a much higher dependence on PDGF than human fibroblasts; the growth of the latter, but not of the former, could be stimulated by the combination of EGF, insulin, and dexamethasone to the full extent of that when PDGF was present; (2) in the absence of PDGF, insulin was an absolute requirement for Swiss 3T3 cells to initiate DNA synthesis, while a substantial proportion of human fibroblasts could enter DNA synthesis without exogenous insulin or IGF-I; and (3) in the absence of PDGF, increasing insulin concentration increased the cycling fraction of Swiss 3T3 cells without an appreciable effect on the rate of cell exit from G0/G1, while under similar culture conditions, insulin showed its major effect on regulation of the G1 exit rate of human fibroblasts, without much effect on the cycling fraction. In addition, the proliferative response of high-density versus low-density, arrested Swiss 3T3 cells showed that the interaction of mitogens varied with cell density. At high cell density, the PDGF requirement was consistent with the "competence/progression" cell-cycle model. This growth response was not seen, however, when cells were plated at low density.
Department of Pathology SM-30, University of Washington, Seattle 98195.
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Chen
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Rabinovitch
D000818:Animals; D002450:Cell Communication / Q000502:physiology; D002452:Cell Count; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003470:Culture Media / Q000032:analysis / Q000494:pharmacology*; D004171:Diploidy; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000032:analysis / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D005434:Flow Cytometry; D006801:Humans; D007328:Insulin / Q000032:analysis / Q000494:pharmacology; D051379:Mice; D008934:Mitogens / Q000032:analysis / Q000494:pharmacology; D008938:Mitosis / Q000187:drug effects / Q000502:physiology*; D010982:Platelet-Derived Growth Factor / Q000032:analysis / Q000494:pharmacology
D003470:Culture Media; D007328:Insulin; D008934:Mitogens; D010982:Platelet-Derived Growth Factor; D004815:Epidermal Growth Factor