Ion-pair chromatography of inositol polyphosphates with N-methylimipramine.
A novel counter-ion, N-methylimipramine, was synthesized and utilized in the separation of inositol phosphates by ion-pair chromatography. The structural identity of the counter-ion was documented by nuclear magnetic resonance spectroscopy. This counter-ion was capable of resolving inositol phosphates isocratically by reversed-phase high-performance liquid chromatography. Solvent polarity and ionic strength markedly affected the retention of the polyphosphorylated inositides. pH, however, was less significant in its effects. Injection of inositol trisphosphate paired to N-methylimipramine into a mobile phase containing tetrabutylammonium ions demonstrated free exchange of the inositide between the counter-ions. This counter-ion may therefore prove useful in defining empirically the mechanisms of ion-pair chromatography.
Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor.
J A
JA
Shayman
F S
FS
Barcelon
D002845:Chromatography / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D007099:Imipramine / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D007295:Inositol Phosphates / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D011865:Radioisotope Dilution Technique; D014316:Tritium
D007295:Inositol Phosphates; D014316:Tritium; C065353:N-methylimipramine; D007099:Imipramine