Chromosomal assignment of mutations by specific chromosome loss in the yeast Saccharomyces cerevisiae.
Yeast 2-microns plasmids were integrated near the centromere of a different chromosome in each of 16 cir0 mapping strains of Saccharomyces cerevisiae. The specific chromosomes containing the integrated 2-microns plasmid DNA were lost at a high frequency after crossing the cir0 strains to cir+ strains. A recessive mutation in a cir+ strain can then be easily assigned to its chromosome using this set of mapping strains, since the phenotype of the recessive mutation will be manifested only in diploids having the integrated 2-microns plasmid and the unmapped mutation on homologous chromosomes.
Wakem
L P
LP
Department of Biochemistry, University of Rochester School of Medicine and Dentistry, New York 14642.
Sherman
F
F
D002503:Centromere; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D004171:Diploidy; D005800:Genes, Fungal; D009154:Mutation; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004271:DNA, Fungal