Characterization of a cDNA clone encoding the complete amino acid sequence of cotton isocitrate lyase.
A cDNA clone encoding the glyoxysomal enzyme isocitrate lyase (ICL) (EC 4.1.3.1) was isolated from a library prepared from cotton (Gossypium hirsutum L.) cotyledon poly(A)+ RNA. The clone is 1893 basepairs (bp) in length and contains a 1728 bp open reading frame encoding a polypeptide of 576 residues (Mr = 64,741). The deduced amino acid sequence of cotton ICL is 85.2%, 90.3% and 41.1% identical to ICL from rapeseed, castor bean and E. coli, respectively. Cotton ICL has a C-terminal tripeptide of A-R-M which is a putative trafficking signal for peroxisome (glyoxysome) proteins.
Turley
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Department of Botany, Arizona State University, Tempe 85287-1601.
Choe
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Trelease
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D000595:Amino Acid Sequence; D001483:Base Sequence; D001937:Brassica; D002367:Ricinus communis; D004926:Escherichia coli; D003368:Gossypium; D007522:Isocitrate Lyase / Q000235:genetics*; D008292:Malate Synthase / Q000235:genetics; D008969:Molecular Sequence Data; D007652:Oxo-Acid-Lyases / Q000235:genetics*; D010947:Plants, Toxic; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D008292:Malate Synthase; D007652:Oxo-Acid-Lyases; D007522:Isocitrate Lyase