Linkage of neutrophil response to a mutagen gene (Nrm-1) to the agouti locus in the rat.
The ACI or BDIV rats responded with decreased or increased neutrophil levels in blood after the N-methyl-N-nitrosourea (MNU) administration. The F1 hybrids had decreased neutrophil counts, and the BDIV x (BDIV x ACI)F1 backcross offspring showed two phenotypes. The sex of the rats and the neutrophil response to MNU assorted independently. The results indicated that the neutrophil response to MNU was regulated by autosomal gene Nrm-1 with two alleles. The Nrm-1d regulates the decrease and the Nrm-1i regulates the increase of neutrophils in blood after the MNU administration. The results were confirmed by the SKUMIX computer program. We found that the Nrm-1 gene was linked to the agouti locus (chi-square = 10.3, P less than 0.001). The map distance between two genes was 33 +/- 5 cM. The Nrm-1 gene thus resides on the linkage group IV of the rat.
Department of Pathology, School of Medicine, University of Pittsburgh, PA 15261.
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D000483:Alleles; D000818:Animals; D003433:Crosses, Genetic; D005260:Female; D005796:Genes; D008040:Genetic Linkage; D007958:Leukocyte Count; D008297:Male; D008770:Methylnitrosourea / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects*; D010641:Phenotype; D051381:Rats; D013045:Species Specificity
D009153:Mutagens; D008770:Methylnitrosourea