Effects of anti-inflammatory agents on mucosal inflammation induced by infection with gram-negative bacteria.
Gram-negative bacterial infections of the urinary tract elicit a mucosal inflammatory response. Interleukin-6 is secreted into the urine, and polymorphonuclear leukocytes (PMNL) are recruited. In the present study we examined the effect of anti-inflammatory agents on these parameters and on bacterial clearance from the kidneys. Dexamethasone reduced interleukin-6 secretion, the PMNL response, and bacterial clearance. Diclofenac abolished the urinary interleukin-6 response but reduced the PMNL response and bacterial clearance only at the highest concentrations. Indomethacin drastically decreased bacterial clearance without the corresponding effect on interleukin-6 production or the PMNL response. The results demonstrate that the inhibition of inflammation impairs bacterial clearance from the kidneys. This is, however, not a direct function of inhibited interleukin-6 production or PMNL recruitment.
Department of Clinical Immunology, University of Göteborg, Sweden.
H
H
Linder
I
I
Engberg
C
C
van Kooten
P
P
de Man
C
C
Svanborg-Edén
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D015169:Colony Count, Microbial; D003907:Dexamethasone / Q000494:pharmacology; D004008:Diclofenac / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000276:immunology; D006090:Gram-Negative Bacteria / Q000276:immunology*; D007213:Indomethacin / Q000494:pharmacology; D015850:Interleukin-6 / Q000096:biosynthesis; D007668:Kidney / Q000276:immunology / Q000382:microbiology; D007700:Kinetics; D007958:Leukocyte Count / Q000187:drug effects; D051379:Mice; D008809:Mice, Inbred C3H; D009092:Mucous Membrane / Q000276:immunology; D001743:Urinary Bladder / Q000382:microbiology; D014556:Urine / Q000166:cytology
D000893:Anti-Inflammatory Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D015850:Interleukin-6; D004008:Diclofenac; D003907:Dexamethasone; D007213:Indomethacin