Lowicryl embedding of gastrointestinal tissues.
In the present study, we modified the technique described by Altman et al. (J. Histochem. Cytochem., 32:1217-1223, 1984) for rapid embedding of tissues in Lowicryl K4M. To attain good sections of the small intestine that contained villi, crypts, submucosa, and external muscle layers, we cut 100 micron slices of the full thickness of the wall with a vibratome before embedment and then deoxygenated the resin and tissue before polymerization. The sections we obtained compared favorably with the quality of sections from conventional resins.
Hagen
S J
SJ
Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts 02115.
D000180:Acrylic Resins; D000199:Actins / Q000648:ultrastructure; D000818:Animals; D006652:Histological Techniques; D007421:Intestine, Small / Q000648:ultrastructure*; D009130:Muscle, Smooth / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains
D000180:Acrylic Resins; D000199:Actins; C037883:Lowicryl 4KM resin