A monoclonal antibody, 4H12, recognizes a surface antigen found on granulated metrial gland cells in the murine decidua.
A monoclonal antibody (MAb), designated 4H12, was selected for reactivity to a surface antigen on PYS-2 teratocarcinoma cells. 4H12 was the product of a fusion of lymphoid cells of a non-immunized pregnant C57BL/6 mouse to NS-1 myeloma cells. Initial studies utilizing immunohistochemistry revealed that MAb 4H12 bound to an antigen found on cells in the decidua basalis of 7-, 8- and 10-day pregnant mice. Antigen-positive cells of 11--19-day pregnant mice were also found predominantly in the decidua. A few antigen-positive cells were found in the labyrinth of the placenta and up against Reichert's membrane. Antigen-positive cells were morphologically and spatially distinct, oval to round with large periodic acid Schiff positive granules. Indirect immunofluorescent (IIF) labeling of decidual cultures showed antigen on the surface of cells that were small, oval to round and adherent. The antigen recognized by MAb 4H12 was removed from tissue sections with trypsin and protease and therefore is suggested to be a protein. We conclude that MAb 4H12 recognizes a surface antigen found on cells historically described as granulated metrial gland (GMG) cells. This MAb should greatly facilitate the further analysis of the life history and function of GMG cells during pregnancy.
Department of Biological Structure, School of Medicine, University of Washington, Seattle 98195.
P A
PA
Linnemeyer
M S
MS
Hamilton
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002469:Cell Separation; D003594:Cytoplasmic Granules; D003656:Decidua / Q000737:chemistry* / Q000166:cytology; D004622:Embryo, Mammalian / Q000737:chemistry; D005260:Female; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000032:analysis*; D008791:Metrial Gland / Q000737:chemistry* / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010920:Placenta / Q000737:chemistry; D011247:Pregnancy
D000911:Antibodies, Monoclonal; D008565:Membrane Proteins