[Chromosomal localization of transforming oncogene BGC-Ha-ras in gastric cancer cell line].
BGC-Ha-ras, isolated from the human gastric cancer cell line BGC-823 and activated by point mutation, is a transforming oncogene. To study whether this oncogene can still be located in the original position of chromosome 11 of BGC-823 cell line, chromosomal in situ hybridization method was used. The results showed that 31% silver grains were localized on chromosome 11, 83% of which on the 11p15 region, and in addition, 10% grains on distal end of the short arm of the other abnormal chromosome. Such results are significant in further study of the relation between gene localization and carcinogenesis.
Hou
Y
Y
Beijing Institute for Cancer Research.
D002461:Cell Line, Transformed; D002871:Chromosome Banding; D002880:Chromosomes, Human, Pair 11; D011905:Genes, ras; D006801:Humans; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured