Complement mediated serum cytotoxicity against oligodendrocytes: a comparison with other cells of the oligodendrocyte-type 2 astrocyte lineage.
Rat oligodendrocytes are known to be susceptible to complement attack when exposed to homologous serum as a consequence of anti-myelin antibody independent classical pathway complement activation and attack. We have now compared this susceptibility with that of other cells of the oligodendrocyte-type 2 astrocyte (O-2A) lineage, and show that while type 2 astrocytes are not sensitive, O-2A progenitors are only relatively resistant to serum cytotoxicity, higher concentrations of complement resulting in cell damage. The implications of these findings for the pathogenesis of demyelinating disease are discussed.
Section of Neurology, University of Cambridge Clinical School, Addenbrooke's Hospital, U.K.
N J
NJ
Scolding
B P
BP
Morgan
A K
AK
Campbell
D A
DA
Compston
D000818:Animals; D001253:Astrocytes / Q000276:immunology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003165:Complement System Proteins / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic; D005455:Fluorescent Antibody Technique; D009186:Myelin Sheath / Q000276:immunology; D009457:Neuroglia / Q000187:drug effects; D009836:Oligodendroglia / Q000187:drug effects / Q000276:immunology*; D011107:Polylysine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D011107:Polylysine; D003165:Complement System Proteins