Monoclonal antibodies to Chlamydia psittaci guinea pig inclusion conjunctivitis (GPIC) strain.
Monoclonal antibodies to a strain of Chlamydia psittaci isolated from guinea pig inclusion conjunctivitis (GPIC) were developed. Only five of the 15 hybridomas isolated produced antibodies specific for the GPIC strain, while seven others produced antibodies which cross reacted with other strains and another species. Strain-specific and species-specific monoclonal antibodies were isotyped as IgG2a and IgG3, respectively. It appears that the GPIC strain has at least two epitopes, one of which is specific for the strain and the other common to the species. These monoclonal reagents may be used to immunotype GPIC agents, better than available methods and may be of potential use in the development of vaccines against chlamydial infections.
Department of Bioscience and Biotechnology, Drexel University, Philadelphia, PA 19104.
P V
PV
Cherian
W E
WE
Magee
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D002691:Chlamydophila psittaci / Q000276:immunology*; D003235:Conjunctivitis, Inclusion / Q000382:microbiology / Q000662:veterinary*; D003429:Cross Reactions; D005260:Female; D006168:Guinea Pigs; D006825:Hybridomas; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D051379:Mice; D008854:Microscopy, Electron; D012376:Rodent Diseases / Q000382:microbiology*; D013045:Species Specificity
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal