[Comparative analysis of the structure and function of recA genes from Serratia marcescens Sb and Escherichia coli K-12].
Nucleotide sequence of the 1276 bp fragment of Serratia marcescens DNA coding for the recASM gene has been determined. This structure was shown to contain an ORF corresponding to a protein with molecular weight of 37766 D. Comparative analysis of the regulatory part of recASM and recAEC (Escherichia coli) demonstrated identity of "-35" and "-10" boxes for these genes and similarity of the SOS box and the enhancer sequences. A comparison of the amino acids sequences of RecASM, RecAEC and RecAPA (Pseudomonas aeruginosa) proteins revealed a great conservatism in the N-terminus and in some structural patches (alpha-helices and beta-sheets) of the RecA proteins predicted by the model of Blanar et al. In contrast, a strong variability of the C-terminus (for the last 25 amino acids, in particular) was revealed. A necessity for definite amino acids composition of the carboxy-terminal end is discussed.
Bakhlanova
I V
IV
Alekseev
A A
AA
Zaĭtsev
E N
EN
Zaĭtseva
E M
EM
Lantsov
V A
VA
D000595:Amino Acid Sequence; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D011938:Rec A Recombinases / Q000235:genetics*; D012706:Serratia marcescens / Q000235:genetics*
D011938:Rec A Recombinases
Sravnitel'nyĭ analiz struktury i funktsiĭ genov recA iz Serratia marcescens Sb i Escherichia coli K-12.