Genotoxic effect of a keto-aldehyde produced by thermal degradation of reducing sugars.
Reductone (HOCH2-CO-CHO), a keto-aldehyde formed, in alkaline pH, by thermal degradation of reducing sugars, blocks cellular respiration and macromolecular biosynthesis, inactivates far-UV (254 nm)-irradiated wild-type E. coli cells, and causes DNA strand breaks. So it may be supposed to be an inducer of SOS functions. Indeed, when Salmonella tester strains TA98, TA100 and TA102 were treated with reductone solutions, without metabolization, a mutagenic effect was detected for all of them. Besides, reductone induced lysogenic E. coli cells and cell filamentation, as measured by the Inductest and the SOS Chromotest. So reductone must be considered a genotoxic drug.
Instituto de Biologia, Universidade do Estado do Rio de Janeiro, Brazil.
J O
JO
Valsa
I
I
Felzenszwalb
A C
AC
de Araujo
R
R
Alcantara-Gomes
D001426:Bacterial Proteins / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects; D029968:Escherichia coli Proteins; D005947:Glucose; D006358:Hot Temperature; D008242:Lysogeny / Q000187:drug effects; D008314:Malonates / Q000633:toxicity*; D008315:Malondialdehyde / Q000031:analogs & derivatives / Q000633:toxicity*; D009153:Mutagens; D013014:SOS Response, Genetics / Q000187:drug effects; D012486:Salmonella typhimurium / Q000187:drug effects
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008314:Malonates; D009153:Mutagens; C053139:sulA protein, E coli; C010778:reductone; D008315:Malondialdehyde; D005947:Glucose