Sarcolectin and interferon in the regulation of cell growth.
Sarcolectin is an endolectin present in a great variety of conjunctival tissues (muscles, cartilage, sarcomas), but also in brain or placental extracts of vertebrates, including primates. When purified to electrophoretical homogeneity as a 65-kd protein, it agglutinates cells and has an affinity for simple sugars. In addition, it is able to inhibit the synthesis of interferon (IFN)-dependent secondary proteins and to restore cells to their status ad primum. The biological effect of Poly(I).Poly(C)-induced feedback interferon is inhibited by the addition of sarcolectins, which also abolishes cellular refractoriness to repeated IFN induction. Similarly, sequential association of, first, Poly(I).Poly(C); 4-5 h later, sarcolectin restores the full capacity of both to promote cell growth, unrestrained by IFN. Indeed, the secondary proteins which are in the process of being synthesized are inhibited. In a great variety of animal cells, sarcolectin can also initiate growth after it has been blocked by IFN. This is not an all-or-none effect, but a balance may be struck by IFN and sarcolectin, depending on their respective concentrations and specific activity. We propose that the coordination of these cellular functions of Poly(I).Poly(C), IFN, and sarcolectin takes place in the form of a triangular growth-regulatory cycle and postulate that they thus maintain a balance during differentiated normal tissue development.
Institut National de la Santé et de la Recherche Médicale, Hôpital Saint Vincent de Paul, Paris, France.
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Chany-Fournier
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Jiang
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Chany
D000818:Animals; D000998:Antiviral Agents / Q000037:antagonists & inhibitors; D002455:Cell Division / Q000502:physiology*; D002460:Cell Line; D004261:DNA Replication; D006801:Humans; D007370:Interferon Type I / Q000037:antagonists & inhibitors / Q000502:physiology*; D007700:Kinetics; D037102:Lectins / Q000502:physiology*; D011070:Poly I-C / Q000037:antagonists & inhibitors
D000998:Antiviral Agents; D007370:Interferon Type I; D037102:Lectins; C039495:sarcolectin; D011070:Poly I-C