Genotoxicity of benzo[a]pyrene, 2-nitrofluorene and airborne particulates in the DNA-repair host-mediated assay.
The genotoxic activity of benzo[a]pyrene (BAP), 2-nitrofluorene (NF) and airborne particulate matter was evaluated in the DNA-repair host-mediated assay after intraperitoneal or intratracheal administration. Dimethylnitrosamine (DMNA), used as a positive control, showed a genotoxic effect after both intraperitoneal and intratracheal administration, the strongest effect being found in liver, followed by lungs and kidneys, whereas a weak effect was observed in the spleen. In general no difference in genotoxicity was found between the 2 administration routes used. For BAP, although clearly positive in vitro, a moderate dose-dependent effect was found only in the liver after intraperitoneal administration. NF, which was positive in vitro both with and without a metabolizing system, produced no genotoxic effect in any of the organs tested after intraperitoneal administration. Extracts of airborne particulate matter which were genotoxic in vitro failed to cause a genotoxic effect in vivo by either route of administration. Possible explanations for the differences between the data obtained in vitro and in vivo are discussed.
Department of Toxicology, Agricultural University, Wageningen, The Netherlands.
G A
GA
Heussen
J G
JG
Post
G M
GM
Alink
D000284:Administration, Oral; D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D001140:Aroclors / Q000633:toxicity; D001564:Benzo(a)pyrene / Q000633:toxicity*; D004260:DNA Repair; D004269:DNA, Bacterial / Q000187:drug effects*; D004128:Dimethylnitrosamine / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000235:genetics; D005449:Fluorenes / Q000633:toxicity*; D007274:Injections, Intraperitoneal; D007668:Kidney / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D008168:Lung / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000187:drug effects
D000393:Air Pollutants; D001140:Aroclors; D004269:DNA, Bacterial; D005449:Fluorenes; D001564:Benzo(a)pyrene; D004128:Dimethylnitrosamine