Demonstration of RNA N-glycosidase activity of a Vero toxin (VT2 variant) produced by Escherichia coli O91:H21 from a patient with the hemolytic uremic syndrome.
A new Vero toxin purified from Escherichia coli O91:H21 isolated from a patient with the hemolytic uremic syndrome (VT2vh) was shown to inhibit elongation factor 1-dependent aminoacyl-tRNA binding to ribosomes, resulting in inhibition of protein synthesis in rabbit reticulocytes. VT2vh, like Shiga toxin, VT1 and VT2, showed RNA N-glycosidase activity and cleaved the N-glycosidic bond of the adenosine residue at position 4324 in 28S ribosomal RNA.
Faculty of Pharmaceutical Sciences, Chiba University.
M
M
Furutani
K
K
Ito
Y
Y
Oku
Y
Y
Takeda
K
K
Igarashi
D000818:Animals; D001427:Bacterial Toxins / Q000378:metabolism* / Q000633:toxicity; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D005914:Globins / Q000096:biosynthesis; D006463:Hemolytic-Uremic Syndrome / Q000382:microbiology*; D006801:Humans; D008969:Molecular Sequence Data; D009699:N-Glycosyl Hydrolases / Q000378:metabolism*; D010452:Peptide Biosynthesis; D010455:Peptides; D012335:RNA, Ribosomal / Q000378:metabolism*; D012339:RNA, Ribosomal, 28S / Q000378:metabolism*; D012360:RNA, Transfer, Phe / Q000378:metabolism; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D054788:Ribosome Inactivating Proteins; D012270:Ribosomes / Q000378:metabolism; D022622:Shiga Toxin 1
D001427:Bacterial Toxins; D010455:Peptides; D012335:RNA, Ribosomal; D012339:RNA, Ribosomal, 28S; D012360:RNA, Transfer, Phe; D022622:Shiga Toxin 1; C012001:polyphenylalanine; D005914:Globins; D009699:N-Glycosyl Hydrolases; D054788:Ribosome Inactivating Proteins