Elevated expression of Pgp-1 (Ly-24) by murine peritoneal B lymphocytes.
B cell expression of the surface glycoprotein, Pgp-1 (Ly-24), was evaluated using flow cytometric analysis. Pgp-1 expression on naive, conventional (splenic) B cells was low but could be increased by mitogenic stimulation. Pgp-1 expression on naive peritoneal B cells was higher than expression by unmanipulated conventional B cells, suggesting the possibility that peritoneal B cells have been activated in vivo. However, the elevated expression of Pgp-1 by peritoneal B cells was not accompanied by increased expression of surface Ia but was correlated with Ly-1 status. Further, Pgp-1 expression by B cells from germ-free mice did not differ from that of normal animals. The elevated expression of Pgp-1 by peritoneal B cells is superficially similar to that provoked by mitogenic stimulation of conventional B cells; however, several characteristics suggest that the elevated expression of Pgp-1 by peritoneal B cells does not reflect prior activation of these cells.
Evans Memorial Department of Clinical Research, Boston University Medical Center, MA.
T P
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Murphy
D L
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Kolber
T L
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Rothstein
D000818:Animals; D000950:Antigens, Ly / Q000032:analysis; D000954:Antigens, Surface / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D005434:Flow Cytometry; D005856:Germ-Free Life; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000276:immunology; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010529:Peritoneal Cavity / Q000166:cytology; D016201:Receptors, Lymphocyte Homing; D013154:Spleen / Q000166:cytology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000950:Antigens, Ly; D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II; D008562:Membrane Glycoproteins; D016201:Receptors, Lymphocyte Homing; D013755:Tetradecanoylphorbol Acetate