Insulin provokes apparent increases in rat adipocyte M-kinase.
Insulin has been shown to stimulate the translocation of protein kinase C (PKC) in rat adipocytes. Presently, we found that decreases in cytosolic 80 kDa PKC were associated with increases in Ca++/phospholipid-independent protein kinase activity, and increases in a 50 kDa cytosolic protein recognized by anti-PKC antiserum. These findings suggest that insulin provokes increases in M-kinase in rat adipocytes.
Ishizuka
T
T
James A. Haley Veterans' Hospital, Tampa, FL 33612.
Cooper
D R
DR
Farese
R V
RV
D000273:Adipose Tissue / Q000201:enzymology*; D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D015153:Blotting, Western; D003600:Cytosol / Q000201:enzymology; D007328:Insulin / Q000494:pharmacology*; D008970:Molecular Weight; D010446:Peptide Fragments / Q000276:immunology / Q000378:metabolism; D011493:Protein Kinase C / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors
D000470:Alkaloids; D007328:Insulin; D010446:Peptide Fragments; D011493:Protein Kinase C; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate