[The induction of differentiation of myeloid antigen positive acute lymphoblastic leukemia by phorbol ester (TPA) or GM-CSF].
In 8 cases of myeloid antigen positive (My+) ALL (L2), morphology and phenotype of their blasts and the differentiation-inducing effect of each of 12-o-tetradecanoyl phorbol-13-acetate (TPA) or recombinant GM-CSF (rGM-CSF) on the blasts were analysed. Five cases expressed common ALL phenotype such as CD19+, CD20-(-)+, CD10+, DR+ and c-mu-(-)+, and also My antigens such as CD11c+, CD11b+, CD14+ and CD13+ (B+My+ALL). One case expressed T-ALL phenotype such as CD5+, CD2+, CD3+, CD8+, CD4+ and CD10+ and also My antigen such as CD14 and CD13 (T+My+ALL). These six cases were diagnosed as biphenotypic ALL. The other 2 cases expressed mainly My antigen such as CD11c+, CD11b+, CD14+, CD13+, CD33+ and DR+, and one of the two expressed also CD2 (B-T-(-)+My+ALL). TPA induced the marked morphological change to monocyte-macrophage like cells and the increase of CD11+ cells in both of B-T-(-)+My+ALL cases. rGM-CSF transformed the blasts to large cells or cells with azurephilic granules in B+My+ALL cases.
Takeuchi
H
H
First Department of Pathology, Saitama Medical School.
Katayama
I
I
D000293:Adolescent; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis; D001853:Bone Marrow / Q000276:immunology*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010703:Phorbol Esters / Q000494:pharmacology*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology / Q000473:pathology*; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D000954:Antigens, Surface; D003115:Colony-Stimulating Factors; D006133:Growth Substances; D010703:Phorbol Esters; D016178:Granulocyte-Macrophage Colony-Stimulating Factor