A candidate protein kinase C gene, PKC1, is required for the S. cerevisiae cell cycle.
Probes derived from cDNAs encoding isozymes of rat protein kinase C (PKC) were used to screen the genome of the budding yeast S. cerevisiae. A single gene (PKC1) was isolated that encodes a putative protein kinase closely related to the alpha, beta, and gamma subspecies of mammalian PKC. Deletion of PKC1 resulted in recessive lethality. Cells depleted of the PKC1 gene product displayed a uniform phenotype, a characteristic of cell division cycle (cdc) mutants, and arrested cell division at a point subsequent to DNA replication, but prior to mitosis. Unlike most cdc mutants, which continue to grow in the absence of cell division, PKC1-depleted cells arrested growth with small buds. PKC1 may regulate a previously unrecognized checkpoint in the cell cycle.
Department of Microbiology and Immunology, University of California, San Francisco 94143.
D E
DE
Levin
F O
FO
Fields
R
R
Kunisawa
J M
JM
Bishop
J
J
Thorner
D000595:Amino Acid Sequence; D001483:Base Sequence; D002453:Cell Cycle; D002874:Chromosome Mapping; D015870:Gene Expression; D005800:Genes, Fungal; D008040:Genetic Linkage; D008969:Molecular Sequence Data; D011493:Protein Kinase C / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D011995:Recombination, Genetic; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics
D012333:RNA, Messenger; D011493:Protein Kinase C