Bioreactor development for production of viral pesticides or heterologous proteins in insect cell cultures.
The insect cell-baculovirus expression system has significant potential for producing proteins requiring some degree of posttranslational modification. T. ni cells appear to be as good a host as S. frugiperda cells for heterologous protein production as demonstrated by production of beta-galactosidase. Attachment-dependent cells of T. ni can be effectively cultured in a packed-bed reactor using glass beads. When cell in such a reactor were infected, they produced 35% of the total protein as beta-galactosidase. No cell detachment was observed even 70 h postinfection. A model of viral entry has been proposed and tested.
Shuler
M L
ML
School of Chemical Engineering, Cornell University, Ithaca, New York 14853.
Cho
T
T
Wickham
T
T
Ogonah
O
O
Kool
M
M
Hammer
D A
DA
Granados
R R
RR
Wood
H A
HA
D000818:Animals; D002478:Cells, Cultured; D007304:Insect Viruses / Q000502:physiology*; D007313:Insecta / Q000166:cytology*; D008954:Models, Biological; D011994:Recombinant Proteins / Q000096:biosynthesis*; D014776:Virus Cultivation; D014779:Virus Replication / Q000502:physiology*
D011994:Recombinant Proteins