A high-performance liquid chromatography method for separation of purine bases, nucleosides and ureides: application to studies on purine catabolism in higher plants.
Mixtures of purine nucleotides, nucleosides, nucleobases, uric acid, allantoin and allantoic acid were fractionated by high-performance liquid chromatography on a polyvinyl alcohol gel column, Asahipak GS-320H, with isocratic elution by sodium phosphate. Application of this system to the determination of the sizes of cellular pools of purine derivatives in plant cells and of the activity of related enzymes, as well as to the purification of enzymatically synthesized radioactive compounds, is described.
Department of Biology, Faculty of Science, Ochanomizu University, Tokyo, Japan.
H
H
Ashihara
N
N
Yabuki
K
K
Mitsui
D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D010944:Plants / Q000378:metabolism*; D011684:Purine Nucleosides / Q000302:isolation & purification*; D011685:Purine Nucleotides / Q000302:isolation & purification*; D011687:Purines / Q000302:isolation & purification* / Q000378:metabolism; D014527:Uric Acid / Q000302:isolation & purification*
D011684:Purine Nucleosides; D011685:Purine Nucleotides; D011687:Purines; D014527:Uric Acid