[The capacity to produce cholera exotoxin in natural strains of Vibrio cholerae].
The study of 27 V. cholerae strains, isolated from cholera patients and found to be hemolytically inactive, with a view to establish their capacity for the production of cholera toxin has revealed that 4 strains (V. cholerae cholerae Dacca 35, V. cholerae cholerae Dacca 3, V. cholerae cholerae B1307, V. cholerae cholerae J89) produce this protein. The quantitative determination of enterotoxin has been made with the use of GM1 ELISA technique. Strain Dacca 35 has been found to be highly toxigenic and, as regards the amount of exotoxin it produces, no different from V. cholerae cholerae strain 569B, a well-known producer of cholera toxin. In strain Dacca 35 correlation between the capacity of the cells for toxin production and the morphology of colonies has been established. The study has revealed that the chromosome of strain Dacca 35 contains two copies of gene vctAB responsible for the synthesis of cholera toxin.
Smirnova
N I
NI
Livanova
L F
LF
Eroshenko
G A
GA
D000818:Animals; D000833:Animals, Suckling; D015373:Bacterial Typing Techniques; D002772:Cholera Toxin / Q000032:analysis / Q000096:biosynthesis*; D002876:Chromosomes, Bacterial / Q000648:ultrastructure; D004269:DNA, Bacterial / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005798:Genes, Bacterial / Q000235:genetics; D006462:Hemolytic Plaque Technique; D009876:Operon / Q000235:genetics; D011817:Rabbits; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity / Q000648:ultrastructure; D014774:Virulence
D004269:DNA, Bacterial; D002772:Cholera Toxin
Izuchenie sposobnosti k produktsii kholernogo ékzotoksina u prirodnykh shtammov Vibrio cholerae.