A novel oncofetal gene is expressed in a stage-specific manner in murine embryonic development.
A novel cDNA clone obtained from a murine T-lymphoma library hybridizes to transcripts expressed in placenta and embryos (Pem) in a stage-specific manner. The Pem cDNA sequence predicts an intracellular hydrophilic protein with no significant sequence similarity to other DNA or protein sequences. Pem transcripts are abundant in 7- and 8-day mouse embryos, but decrease precipitously thereafter. On Day 9 they become abundant in placenta and yolk sac, persisting there until parturition. Although Pem transcripts are present in immortalized and tumorigenic cell lines from several different cell lineages, they are not detectable in any of 15 adult tissues tested. The expression of Pem during fetal development and its presence in immortalized and neoplastic cell lines is consistent with the properties expected of an "oncofetal" gene.
University of California, San Diego Cancer Center, La Jolla 92093.
M F
MF
Wilkinson
J
J
Kleeman
J
J
Richards
C L
CL
MacLeod
D000595:Amino Acid Sequence; D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004622:Embryo, Mammalian / Q000737:chemistry; D005314:Embryonic and Fetal Development; D005333:Fetus; D005786:Gene Expression Regulation; D015723:Gene Library; D008223:Lymphoma / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D010920:Placenta / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D014158:Transcription, Genetic; D015017:Yolk Sac / Q000737:chemistry
D000951:Antigens, Neoplasm; D012333:RNA, Messenger; C031192:oncofetal antigens