Schistosoma mansoni: localization of the 28 kDa secreted protease in cercaria.
Monospecific rabbit antibodies were utilized to localize the 28 kDa serine protease which is released from transforming schistosomula of Schistosoma mansoni in cercariae and freshly transformed schistosomula. This protease exerts two postulated activities, degradation of connective tissue proteins thus promoting skin penetration and release of the cercarial glycocalyx leading to accelerated schistosomular transformation. Upon immunogold labelling of cercarial cryosections, the 28 kDa protease was found stored in both the preacetabular and postacetabular glands. This enzyme was also detected in the cercarial glycocalyx by immunogold and immunofluorescence labelling and by its proteolytic activity. Following transformation and shedding of the glycocalyx, the same 28 kDa protease was found on the surface membrane of transformed schistosomula which are resistant to immune damage. It is suggested that the 28 kDa membrane protease which cleaves in vitro the complement proteins C3, C3b and C9, may promote in vivo immunoresistance of S. mansoni.
Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
M
M
Marikovsky
R
R
Arnon
Z
Z
Fishelson
D000818:Animals; D000947:Antigens, Helminth; D001700:Biomphalaria; D015153:Blotting, Western; D003546:Cysteine Endopeptidases / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D015801:Helminth Proteins; D007150:Immunohistochemistry; D051379:Mice; D008813:Mice, Inbred ICR; D008970:Molecular Weight; D011817:Rabbits; D012550:Schistosoma mansoni / Q000201:enzymology* / Q000648:ultrastructure; D012697:Serine Endopeptidases / Q000032:analysis*
D000947:Antigens, Helminth; D015801:Helminth Proteins; C052358:P28 antigen, Schistosoma; D012697:Serine Endopeptidases; D003546:Cysteine Endopeptidases