Oligodeoxynucleoside phosphorothioate stability in subcellular extracts, culture media, sera and cerebrospinal fluid.
Degradation of a synthetic oligodeoxynucleoside phosphorothioate was studied in six systems used for antisense inhibition experiments. Oligodeoxynucleoside phosphorothioates were degraded very slowly at 37 degrees C in all of the systems studied. Measured half-lives of pentadecamers were 12 +/- 1 h in rabbit reticulocyte lysate, 7 +/- 1 h in HeLa cell postmitochondrial extract, 14 +/- 2 h in RPMI 1640 with 10% fetal bovine serum, 8 +/- 1 h in undiluted fetal bovine serum, 9 +/- 1 h in adult human serum, and 19 +/- 7 h in rat cerebrospinal fluid.
Department of Chemistry, University of South Florida, Tampa 33620.
J M
JM
Campbell
T A
TA
Bacon
E
E
Wickstrom
D000818:Animals; D001483:Base Sequence; D002457:Cell Extracts / Q000032:analysis; D003470:Culture Media; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000378:metabolism; D016376:Oligonucleotides, Antisense; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc; D011817:Rabbits; D051381:Rats; D012156:Reticulocytes / Q000378:metabolism; D013873:Thionucleotides / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism*; D013997:Time Factors
D002457:Cell Extracts; D003470:Culture Media; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D013873:Thionucleotides