Use of a new simplified assay for phospholipase A2 to measure bacterial enzyme levels.
During investigation of possible phospholipase A2 (PLA2) production by pathogenic bacteria associated with preterm labour, a rapid and simple assay method was developed which involved few steps and which could be applied easily to large numbers of samples. The principle difference from previously described methods lies in separation of the reaction products by partitioning them between organic and aqueous solvents, rather than by using thin layer chromatography. This enabled us to determine that none of the bacteria studied released PLA2 into the culture medium spontaneously, and that only Escherichia coli contained high levels of PLA2.
Institute of Obstetrics & Gynaecology, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
M R
MR
Lumb
C K
CK
Roseblade
R
R
Helmig
N
N
Uldbjerg
M H
MH
Sullivan
M G
MG
Elder
D002250:Carbon Radioisotopes; D004926:Escherichia coli / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007778:Lactobacillus / Q000201:enzymology*; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D013292:Streptococcus agalactiae / Q000201:enzymology*; D013997:Time Factors
D002250:Carbon Radioisotopes; D009829:Oleic Acids; D010713:Phosphatidylcholines; D019301:Oleic Acid; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; C028694:1-palmitoyl-2-oleoylphosphatidylcholine