[Immunogold and immunofluorescence localization of epidermal growth factor receptors on MCF-7 human breast cancer cells].
Immunogold and immunofluorescence staining techniques were used for localization of epidermal growth factor (EGF) receptors on MCF-7 cell membranes. Fluorescence microscopy showed that MCF-7 cells reacted with the monoclonal antibody against EGF receptors and gave positive reaction on the membrane surface. Under electron microscope, the electron-dense gold particles were distributing on the membrane surface including the microvilli. A continued course of internalization of EGF receptors labeled with colloidal gold was observed by electron microscopy. It is considered that one of the mechanisms involving breast cancer MCF-7 cell growth and the stimulating effect on the growth of MCF-7 cells by estradiol is autocrine secretion.
Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing.
X M
XM
Zhang
D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D066246:ErbB Receptors / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D014407:Tumor Cells, Cultured / Q000378:metabolism
D066246:ErbB Receptors