Yeast KRE genes provide evidence for a pathway of cell wall beta-glucan assembly.
The Saccharomyces cerevisiae KRE1 gene encodes a Ser/Thr-rich protein, that is directed into the yeast secretory pathway, where it is highly modified, probably through addition of O-linked mannose residues. Gene disruption of the KRE1 locus leads to a 40% reduced level of cell wall (1----6)-beta-glucan. Structural analysis of the (1----6)-beta-glucan fraction, isolated from a strain with a krel disruption mutation, showed that it had an altered structure with a smaller average polymer size. Mutations in two other loci, KRE5 and KRE6 also lead to a defect in cell wall (1----6)-beta-glucan production and appear to be epistatic to KRE1. These findings outline a possible pathway of assembly of yeast cell wall (1----6)-beta-glucan.
Department of Biology, McGill University, Montreal, Quebec, Canada.
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D000595:Amino Acid Sequence; D001483:Base Sequence; D002473:Cell Wall / Q000378:metabolism / Q000648:ultrastructure; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics*; D005936:Glucans / Q000378:metabolism*; D008562:Membrane Glycoproteins; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009154:Mutation; D009183:Mycotoxins / Q000494:pharmacology; D021382:Protein Sorting Signals / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins
D005656:Fungal Proteins; D005936:Glucans; C064013:KRE1 protein, S cerevisiae; D008562:Membrane Glycoproteins; D009183:Mycotoxins; D021382:Protein Sorting Signals; D029701:Saccharomyces cerevisiae Proteins