Validity testing of commercial urine cocaine metabolite assays: IV. Evaluation of the EMIT d.a.u. cocaine metabolite assay in a quantitative mode for detection of cocaine metabolite.
The EMIT d.a.u. cocaine metabolite assay (EMIT dau) was evaluated in a quantitative mode for analysis of clinical specimens obtained after controlled cocaine administration to human subjects. The quantitative results showed high concordance with those of gas chromatography/mass spectrometry (GC/MS) assays of the same specimens for benzoylecgonine, and no false positive or false negative results were obtained. The evaluation also included analysis of standardized solutions containing benzoylecgonine, cocaine, and other cocaine metabolites and isomers. The EMIT dau antibody demonstrated high selectivity for benzoylecgonine. The precision was somewhat less than that reported earlier for other commercial cocaine metabolite immunoassays. Quantitation of initial screening results from EMIT dau testing can serve as a useful guide for confirmation by GC/MS in forensic science urine testing.
Laboratory of Chemistry and Drug Metabolism, National Institute on Drug Abuse, Baltimore, MD.
E J
EJ
Cone
D
D
Yousefnejad
S L
SL
Dickerson
D003042:Cocaine / Q000008:administration & dosage / Q000378:metabolism / Q000652:urine*; D003429:Cross Reactions; D005069:Evaluation Studies as Topic; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007124:Immunoenzyme Techniques; D012044:Regression Analysis; D015203:Reproducibility of Results
D003042:Cocaine