The ostrich pituitary contains a major peptide homologous to mammalian chromogranin A(1-76).
A major peptide related to the NH2-terminal fragment (position 1 to 76) of mammalian chromogranin A was isolated from ostrich adenohypophyses following acid-acetone extraction. The complete amino acid sequence of the homogenous peptide was deduced following automatic Edman degradation of the native peptide as well as of CNBr-, tryptic- and Lysobacter-derived peptides. The 76 amino acid sequence is strikingly homologous to bovine (80.3% sequence identity), porcine (79.0%), human (79.0%) and rat (72.4%) corresponding sequences, but much less so to human chromogranin B (22.4%). As this peptide is followed in bovine, porcine and human structure by a pair of basic residues (Lys-Lys), it could conceivably be produced during maturation in secretory granules. Finally, its structure appears to contain two potential amphipathic helices joined by the single disulfide bridge present in all chromogranin A and B molecules.
J. A. De Sève Laboratory of Molecular Neuroendocrinology, Clinical Research Institute of Montréal, Québec, Canada.
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Lazure
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Paquet
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Litthauer
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Naudé
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Oelofsen
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Chrétien
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001717:Birds / Q000378:metabolism*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D053379:Chromogranin A; D002864:Chromogranins / Q000302:isolation & purification*; D003488:Cyanogen Bromide; D006801:Humans; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000302:isolation & purification*; D010447:Peptide Hydrolases; D010455:Peptides / Q000302:isolation & purification*; D010903:Pituitary Gland, Anterior / Q000032:analysis*; D051381:Rats; D013045:Species Specificity; D013552:Swine
D000596:Amino Acids; D053379:Chromogranin A; D002864:Chromogranins; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D010455:Peptides; C064110:vasostatin I; D010447:Peptide Hydrolases; D003488:Cyanogen Bromide