Enzyme-linked immunosorbent assay (ELISA) for detection of antibodies against Pityrosporum orbiculare.
IgG antibodies to Pityrosporum orbiculare were detected by an enzyme-linked immunosorbent assay (ELISA) in sera from 10 healthy adults and five healthy 6-month-old children. The ELISA showed 17-80 times higher antibody levels in adults than were found by an indirect immunofluorescence technique (IIF) used in earlier studies. Low titres of antibody were detected in the children.
Johansson
S
S
Department of Clinical Immunology, Karolinska Institute, Karolinska Hospital, Stockholm, Sweden.
Faergemann
J
J
D000328:Adult; D000908:Antibodies, Fungal / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007223:Infant; D008290:Malassezia / Q000276:immunology*
D000908:Antibodies, Fungal; D007074:Immunoglobulin G