[A primary study on molecular epidemiology of diarrhea caused by enterotoxigenic Escherichia coli by DNA colony hybridization using three enterotoxigenic gene-probes].
921 isolates of acute-diarrhea-inducing E. Coli strains from several provinces and cities, including Guangzhou, Wuhan, Sichuan, Shenyang and Hunan, have been collected during 1984-1989 and were identified in 1989 for detecting genes coding for these enterotoxins-LT, ST-P, ST-H by DNA colony hybridization using three enterotoxigenic gene probes. Of all the isolates homologous to these genes encoding LT, ST-P, ST-H and both LT and ST were 198 (21.5%), 131 (14.2%), 54 (5.9%) and 18 (2.1%), respectively. It was showed that in our country pathogenic agents of acute infectious diarrheal disease with ETEC strains first might be ETEC-LT strains, secondary ETEC-ST-H strains. Whereas, in rural districts ETEC-ST-P possess higher proportion of ETEC strains. These results provided some essential data and advanced methods for researching further molecular epidemiology of the ETEC diarrheal disease.
Dept. of Epidemiology, First Military Medical College.
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D002681:China / Q000453:epidemiology; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D003967:Diarrhea / Q000453:epidemiology* / Q000382:microbiology; D004768:Enterotoxins / Q000235:genetics; D004926:Escherichia coli / Q000032:analysis / Q000145:classification*; D004927:Escherichia coli Infections; D006801:Humans
D015342:DNA Probes; D004269:DNA, Bacterial; D004768:Enterotoxins