Transcriptional control of c-myc gene expression during stimulation of murine B lymphocytes.
The binding of ligand to surface IgR results in the initial activation of B cells. As shown by experiments in which B cells are polyclonally stimulated with anti-Ig antibody, this includes an early increase in c-myc gene expression. In our study a correlation between increases in the rate of gene transcription and the level of c-myc mRNA was observed both with the brief increase in c-myc expression that is induced by anti-Ig, as well as with the more intense and prolonged expression of c-myc that follows treatment with anti-Ig plus the cytoskeleton perturbing agent, cytochalasin. Elevation of the rate of initiation was detected with both stimulatory regimens although the combination of anti-Ig plus cytochalasin increased the rate of elongation in addition to the rate of initiation. These results suggest that stimulation of B lymphocytes alters expression of trans-acting factors that regulate transcription of the c-myc gene.
Buckler
A J
AJ
Department of Biochemistry, Boston University School of Medicine, MA 02118.
Rothstein
T L
TL
Sonenshein
G E
GE
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D015638:Cytochalasin D / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D066298:In Vitro Techniques; D051379:Mice; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger / Q000235:genetics; D011947:Receptors, Antigen, B-Cell / Q000502:physiology; D015183:Restriction Mapping; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects
D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D011947:Receptors, Antigen, B-Cell; D015638:Cytochalasin D