Reactivity of primary biliary cirrhosis sera with Escherichia coli dihydrolipoamide acetyltransferase (E2p): characterization of the main immunogenic region.
Primary biliary cirrhosis (PBC) is a chronic cholestatic liver disease characterized by the presence of antimitochondrial autoantibodies in the serum. The major antigens recognized by the antibodies are the E2 components of the 2-oxo acid dehydrogenase complexes, all of which possess covalently attached lipoic acid cofactors. A bacterial etiology has been proposed for the disease, and patients' antibodies are known to recognize the E2 subunits (E2p) of both mammalian and bacterial pyruvate dehydrogenase complexes. Immunoblotting and ELISA inhibition techniques using extracts of Escherichia coli deletion strains, genetically restructured E2 polypeptides, and isolated lipoyl domains demonstrate that (i) the E2o subunit of the E. coli 2-oxoglutarate dehydrogenase complex is recognized by patients' antibodies; (ii) the main immunogenic region of E2p lies within the lipoly domains; (iii) the presence of a lipoly residue within the domain is crucial for effective recognition by the antibodies; and (iv) octanoylated E2p, octanoylated E2o, and octanoylated lipoyl domain, produced by a mutant deficient in lipoate biosynthesis, are recognized by patients' antibodies but not as effectively as their lipoylated counterparts. These findings indicate that antibodies in PBC patients' sera bind to a unique peptide-cofactor conformation within the lipoyl domains of the E2 polypeptides and that this epitope is partially mimicked by substituting the lipoyl cofactor with an octanoyl group.
Department of Biochemistry, Medical School, University of Newcastle upon Tyne, United Kingdom.
S P
SP
Fussey
S T
ST
Ali
J R
JR
Guest
O F
OF
James
M F
MF
Bassendine
S J
SJ
Yeaman
D000123:Acetyltransferases / Q000276:immunology*; D000595:Amino Acid Sequence; D000936:Antigen-Antibody Complex / Q000032:analysis; D000937:Antigen-Antibody Reactions; D001323:Autoantibodies / Q000302:isolation & purification*; D051047:Dihydrolipoyllysine-Residue Acetyltransferase; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000201:enzymology; D006801:Humans; D015151:Immunoblotting; D007655:Ketoglutarate Dehydrogenase Complex / Q000276:immunology; D008105:Liver Cirrhosis, Biliary / Q000097:blood / Q000276:immunology*; D046911:Macromolecular Substances; D008930:Mitochondria, Liver / Q000276:immunology; D008969:Molecular Sequence Data; D011768:Pyruvate Dehydrogenase Complex / Q000276:immunology
D000936:Antigen-Antibody Complex; D001323:Autoantibodies; D046911:Macromolecular Substances; D011768:Pyruvate Dehydrogenase Complex; D007655:Ketoglutarate Dehydrogenase Complex; D000123:Acetyltransferases; D051047:Dihydrolipoyllysine-Residue Acetyltransferase