Numerical analysis of SDS-PAGE protein patterns of Serratia marcescens: a comparison with other typing methods.
Twenty-five cultures comprising 18 clinical isolates of Serratia marcescens from two hospitals, the type strain of S. marcescens, two reference strains of S. marinorubra, the type or a reference strain of three other Serratia species and a reference strain of undetermined species, were characterized by one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of whole-cell proteins. The protein patterns were highly reproducible and were used as the basis of a numerical analysis which divided the clinical isolates into eight protein types. Comparison with O-serotyping indicated that the level of discrimination by SDS-PAGE was similar. As with O-serotyping, a secondary scheme, such as phage typing, is necessary to differentiate strains of the same protein type. We conclude that high-resolution SDS-PAGE of proteins provides an effective adjunct to other methods for typing isolates of S. marcescens.
Holmes
B
B
National Collection of Type Cultures, Central Public Health Laboratory, London, England.
Costas
M
M
Sloss
L L
LL
D001426:Bacterial Proteins / Q000032:analysis*; D001434:Bacteriophage Typing; D003428:Cross Infection / Q000382:microbiology; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D006801:Humans; D015203:Reproducibility of Results; D012703:Serotyping; D012706:Serratia marcescens / Q000032:analysis / Q000145:classification*
D001426:Bacterial Proteins