Induction of c-Ha-ras gene expression by double-stranded RNA and interferon requirement.
The addition of double-stranded RNA (dsRNA) to NIH 3T3 cells led to an increase in the RNA levels of c-Ha-ras. The double-stranded configuration was required for the increase in c-Ha-ras mRNA levels, as heat-denatured dsRNA and single-stranded RNA did not have any effect. Nuclear run-on transcription experiments indicated that the increase in c-Ha-ras mRNA levels stimulated by dsRNA was due to transcriptional activation of the gene. The induction of c-Ha-ras gene expression by dsRNA was inhibited by anti-beta interferon antibodies, suggesting that interferon might mediate the induction.
Long Island Jewish Medical Center, New Hyde Park, New York 11042.
A
A
Maran
I D
ID
Goldberg
B M
BM
Steinberg
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D015870:Gene Expression / Q000187:drug effects; D011905:Genes, ras / Q000187:drug effects; D007106:Immune Sera; D007370:Interferon Type I / Q000276:immunology / Q000502:physiology; D007700:Kinetics; D051379:Mice; D011070:Poly I-C / Q000494:pharmacology*; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016283:Proto-Oncogene Proteins p21(ras); D012330:RNA, Double-Stranded / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D014158:Transcription, Genetic
D007106:Immune Sera; D007370:Interferon Type I; D011518:Proto-Oncogene Proteins; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D011505:Protein-Tyrosine Kinases; D016283:Proto-Oncogene Proteins p21(ras); D011070:Poly I-C