A new, highly sensitive enzymic assay for human tryptase and its use for identification of tryptase inhibitors.
A chromogenic two-stage assay for human tryptase, a specific marker of mast cell activation, was developed based on the tryptase-induced conversion of prothrombin to thrombin. This assay proved to be more sensitive and reliable than measurements of amidolytic activity of tryptase with small synthetic substrates such as Bz-Arg-Nan and was suitable to detect tryptase activity in human body fluids. In addition, the assay was useful for studies of natural and recombinant inhibitors of tryptase.
Dietze
S C
SC
Department of Clinical Chemistry and Clinical Biochemistry, University of Munich, FRG.
Auerswald
E A
EA
Fritz
H
H
D001711:Biotransformation; D004796:Clinical Enzyme Tests / Q000379:methods; D005260:Female; D006801:Humans; D008407:Mast Cells / Q000201:enzymology; D010447:Peptide Hydrolases / Q000032:analysis*; D011480:Protease Inhibitors / Q000032:analysis*; D011516:Prothrombin / Q000378:metabolism
D011480:Protease Inhibitors; D011516:Prothrombin; D010447:Peptide Hydrolases; C023073:tosylarginine methyl ester hydrolase