Occurrence, characteristics, and patterns of HIV-1 and HIV-2 western blot indeterminate sera in low risk populations in West Virginia and pre-AIDS Africa.
To further characterize HIV-1 and HIV-2 Western blot indeterminate (IWB) sera, 402 sera from 318 healthy low-risk individuals from West Virginia and 159 African sera obtained in the pre-AIDS era (1968-1972) were studied. All IWB sera tested for antigen by HIV-1 enzyme immunoassay (EIA-Ag) were negative. HIV-1 and HIV-2 IWB reactivity occurred independent of HIV-1 and HIV-2 false-positive testing for antibody by enzyme immunoassay (EIA-Ab) and no cross-reactions between HIV-1 and HIV-2 IWB patterns were detected. The IWB patterns were reproducible, demonstrated no age or sex related pattern, and showed no evidence of vertical or horizontal transmission. The African sera exhibited a significantly higher number of IWB patterns. IWB reactivity in HIV-1 and HIV-2 seronegative individuals may not be viral in origin and the occurrence of IWB pattern may vary among populations.
Marshall University School of Medicine, Huntington, West Virginia.
A H
AH
Schindzielorz
R B
RB
Belshe
M A
MA
Mufson
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D015153:Blotting, Western; D002648:Child; D002675:Child, Preschool; D005189:False Positive Reactions; D005260:Female; D015483:HIV Antibodies / Q000097:blood*; D006679:HIV Seropositivity; D015497:HIV-1 / Q000276:immunology*; D015498:HIV-2 / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007630:Kenya; D007988:Liberia; D008297:Male; D012307:Risk Factors; D012737:Sex Factors; D014903:West Virginia; D015030:Zimbabwe
D015483:HIV Antibodies