Possession of identical nonconjugative plasmids by different isolates of Pasteurella multocida does not imply clonality.
Experiments were performed to test the hypothesis that possession of the same nonconjugative R plasmid by different isolates of Pasteurella multocida implied that they were of the same clone. Seven isolates of P. multocida were studied, two possessed an identical nonconjugative R plasmid (pVM109), four possessed another (pVM110), and one isolate possessed a nonconjugative R plasmid related to pVM110. Phenotypic and genotypic characteristics of the isolates were determined and compared. Isolates possessing the same nonconjugative R plasmid were shown to be different, and isolates possessing a different nonconjugative R plasmid were shown to be the same. We conclude that possession of an identical nonconjugative R plasmid by two isolates of P. multocida does not imply clonality.
Ikeda
J S
JS
Department of Veterinary Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis 95616.
Hirsh
D C
DC
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005838:Genotype; D009693:Nucleic Acid Hybridization; D010325:Pasteurella / Q000145:classification / Q000235:genetics*; D010641:Phenotype; D011815:R Factors; D015347:RNA Probes; D015183:Restriction Mapping; D014422:Turkeys
D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D015347:RNA Probes