Use of anti-cholera toxin IgA-secreting hybridoma cells for the elaboration of an antigen-specific IgA-ELISPOT-assay.
Hybridoma anti-cholera toxin (CT) IgA-secreting cells, because of their monoclonal character, were used to establish and optimize an ELISPOT-assay for CT-specific IgA-secreting cells. The use of hybridoma cells easily and quickly allowed the elaboration of a sensitive, specific and reproducible ELISPOT-assay, which was successfully applied to enumerate intestinal lamina propria lymphoid cells secreting anti-CT IgA in mice immunized twice intraintestinally with CT. Monoclonal antibody-secreting hybridoma cells, if available against a given antigen, are suggested as ideal cell suspensions to elaborate new ELISPOT-assays to study antibody responses at the cellular level.
Catholic University of Louvain, International Institute of Cellular and Molecular Pathology, Brussels, Belgium.
P M
PM
Solbreux
J P
JP
Vaerman
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D000921:Antibody-Producing Cells / Q000276:immunology*; D002452:Cell Count / Q000379:methods; D002772:Cholera Toxin / Q000276:immunology*; D005069:Evaluation Studies as Topic; D006825:Hybridomas / Q000276:immunology; D007124:Immunoenzyme Techniques; D007071:Immunoglobulin A, Secretory / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D015203:Reproducibility of Results
D000911:Antibodies, Monoclonal; D007071:Immunoglobulin A, Secretory; D002772:Cholera Toxin