Medium scale production of L-myo-inositol 1-phosphate.
L-myo-Inositol-1-phosphate synthetase was purified from baker's yeast, grown in a fermenter in an inositol-deficient medium and analyzed using a new HPLC assay for inositol. This enzyme was used in a procedure, developed from methods partially described in the literature, for the medium scale production and purification of L-myo-inositol 1-phosphate. The identity and purity of the product were confirmed by 1H and 31P NMR spectroscopy.
Gesellschaft für Biotechnologische Forschung, Ag. Enzymologie, Braunschweig, Federal Republic of Germany.
F
F
Feth
R
R
Wagner
H
H
Baydoun
V
V
Wray
K G
KG
Wagner
D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D007295:Inositol Phosphates / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D007296:Myo-Inositol-1-Phosphate Synthase / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000201:enzymology
D007295:Inositol Phosphates; C002647:inositol 1-phosphate; D007296:Myo-Inositol-1-Phosphate Synthase