[A primary study on molecular epidemiology of diarrhea caused by enterotoxigenic Escherichia coli by DNA colony hybridization using three enterotoxigenic gene-probes].
921 isolates of acute-diarrhea-inducing E. Coli strains from several provinces and cities, including Guangzhou, Wuhan, Sichuan, Shenyang and Hunan, have been collected during 1984-1989 and were identified in 1989 for detecting genes coding for these enterotoxins-LT, ST-P, ST-H by DNA colony hybridization using three enterotoxigenic gene probes. Of all the isolates homologous to these genes encoding LT, ST-P, ST-H and both LT and ST were 198 (21.5%), 131 (14.2%), 54 (5.9%) and 18 (2.1%), respectively. It was showed that in our country pathogenic agents of acute infectious diarrheal disease with ETEC strains first might be ETEC-LT strains, secondary ETEC-ST-H strains. Whereas, in rural districts ETEC-ST-P possess higher proportion of ETEC strains. These results provided some essential data and advanced methods for researching further molecular epidemiology of the ETEC diarrheal disease.
Yu
S
S
Dept. of Epidemiology, First Military Medical College.
D002681:China / Q000453:epidemiology; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D003967:Diarrhea / Q000453:epidemiology* / Q000382:microbiology; D004768:Enterotoxins / Q000235:genetics; D004926:Escherichia coli / Q000032:analysis / Q000145:classification*; D004927:Escherichia coli Infections; D006801:Humans
D015342:DNA Probes; D004269:DNA, Bacterial; D004768:Enterotoxins