Myofibrillar protein turnover in feed-restricted and realimented beef cattle.
The objective of this study was to determine the effect of feed restriction and repletion on myofibrillar protein turnover in cattle. Crossbred steer calves (n = 12) about 310 d of age were assigned randomly to a diet of corn and silage that was 1) provided ad libitum for 146 d (ALC) or 2) restricted so steers gained .2 kg/d for 80 d but received ad libitum access to feed thereafter for 66 d (RFC). At 27, 55, 97, 118 and 146 d a 24-h urine sample and a blood sample were obtained. Urine was analyzed for N tau-methylhistidine (N tau-MH), creatinine (C), urea nitrogen (UN) and total nitrogen (TN). Serum samples were analyzed for hydroxyproline (HYP), C and albumin (A). Body weights were lower (P less than .05) in RFC at 55, 97, 118 and 146 d. Excretion of N tau-MH was lower (P less than .05) in the RFC at 27 and 55 d but higher at 118 d. Urinary C excretion was higher in ALC at the last four sample times. Urinary UN and TN excretion were lower (P less than .05) in RFC at 55, 97 and 118 d; urinary UN also was lower (P less than .05) at d 146. Serum A was higher (P less than .05) in ALC at 55 and 118 d, respectively. Serum HYP was higher (P less than .05) in RFC at 27 and 55 d. Calculated myofibrillar protein breakdown rates (FBR) and fractional synthesis rates (FSR) were higher (P less than .05) in RFC at the last two sampling periods; FSR was lower for the RFC at the first sampling period.(ABSTRACT TRUNCATED AT 250 WORDS)
Dept. of Anim. Sci., University of Nebraska, Lincoln 68583-0908.
S J
SJ
Jones
D L
DL
Starkey
C R
CR
Calkins
J D
JD
Crouse
D000821:Animal Feed; D000818:Animals; D001806:Blood Urea Nitrogen; D001835:Body Weight; D002417:Cattle / Q000378:metabolism*; D003404:Creatinine / Q000652:urine; D005508:Food Deprivation / Q000502:physiology*; D006909:Hydroxyproline / Q000097:blood; D008297:Male; D008762:Methylhistidines / Q000652:urine; D009124:Muscle Proteins / Q000096:biosynthesis / Q000378:metabolism*; D009210:Myofibrils / Q000378:metabolism*; D011897:Random Allocation; D012709:Serum Albumin / Q000032:analysis
D008762:Methylhistidines; D009124:Muscle Proteins; D012709:Serum Albumin; D003404:Creatinine; D006909:Hydroxyproline