Characterization of the defect in the Escherichia coli mutT1 mutator gene.
With a probe constructed from the wild-type gene, a DNA fragment containing the entire mutT1 mutator gene was isolated and cloned into pUC18. Nucleotide sequence analysis revealed that the mutator defect was most likely due to an IS1 insertion into the wild-type gene.
McCollum-Pratt Institute, Johns Hopkins University, Baltimore, Maryland 21218.
S K
SK
Bhatnagar
L C
LC
Bullions
G
G
Lew
M J
MJ
Bessman
D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D010957:Plasmids; D015183:Restriction Mapping
D004269:DNA, Bacterial