Antibody-dependent killing of Schistosoma mansoni schistosomula in vitro by starch-elicited murine macrophages. Critical role of the cell surface integrin Mac-1 in killing mediated by the anti-Mr 16,000 mAb B3A.
Starch-elicited murine peritoneal macrophages were able to kill schistosomula in vitro in the presence of a variety of immune sera. Dose response experiments revealed the superior "quality" of serum from mice vaccinated four times with highly irradiated cercariae (4xVMS) in mediating killing at titers comparable to the other sera tested. B3A, a partially protective mAb (IgG3) that recognizes a Mr 16,000 schistosomular surface Ag, mediated higher levels of killing than any of the sera at comparable titers. In contrast, H12, a partially protective mAb (IgG2a; anti-Mr 32,000), and C1C9, a nonprotective McAb (IgG3; anti-Mr 38,000) failed to mediate killing. Two anti-Mac-1 alpha-chain mAb (5C6 and M1/70) mediated substantial dose-dependent blocking of 4xVMS and B3A-mediated macrophage killing. In contrast, a mAb to the Mac-1-associated beta-chain was less effective, whereas the mAb F4/80 did not significantly block killing despite being present on this macrophage population. Although whole 5C6 Ig was the most efficient at inhibiting B3A-mediated killing, 5C6 Fab fragments were still effective at concentrations as low as 0.5 microgram/ml (10 nM). On a molar basis 5C6 appeared to be more effective at blocking 4xVMS-mediated killing than M1/70, while only M1/70 was capable of inhibiting macrophage adherence to schistosomula. These findings, together with the observation that anti-alpha chain mAb were far more effective at blocking killing than the anti-beta-chain mAb, rules out the possibility that 5C6 is nonspecifically inhibiting B3A-FcR interaction. The data also imply a functional relationship between Mac-1 and FcRIII, the receptor for B3A, in macrophage killing.
Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, London University, England.
D A
DA
Vignali
Q D
QD
Bickle
P
P
Crocker
M G
MG
Taylor
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000920:Antibody-Dependent Cell Cytotoxicity; D000943:Antigens, Differentiation / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology*; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000276:immunology; D015801:Helminth Proteins / Q000276:immunology; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D016177:Macrophage-1 Antigen; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D011961:Receptors, Fc / Q000276:immunology; D016028:Receptors, Leukocyte-Adhesion / Q000276:immunology*; D012550:Schistosoma mansoni / Q000276:immunology*
D000909:Antibodies, Helminth; D000943:Antigens, Differentiation; D000947:Antigens, Helminth; D015815:Cell Adhesion Molecules; D015801:Helminth Proteins; D016177:Macrophage-1 Antigen; D011961:Receptors, Fc; D016028:Receptors, Leukocyte-Adhesion