A malaria heat-shock-like determinant expressed on the infected hepatocyte surface is the target of antibody-dependent cell-mediated cytotoxic mechanisms by nonparenchymal liver cells.
Cultured hepatic stages of Plasmodium falciparum and P. yoelii and with a monoclonal antibody recognizing a C-terminal fragment of the P. falciparum heat-shock-like protein (Pfhsp70) revealed that synthesis of this antigen first occurs during intrahepatic development of the parasite, at the two nuclei stage. Using a variety of techniques, including scanning electron microscopy, we observed that this antigenic determinant was expressed on the infected hepatocyte membrane. Its participation in antibody-dependent cell-mediated cytotoxicity was investigated. While no effect was obtained with peripheral blood cells, we found that 25% of the schizonts were specifically lysed when using spleen cells at a killer/target ratio of 30/1. More interestingly, with nonparenchymal liver cells, up to 50% of the hepatic parasites disappeared with a killer/target ratio of 10/1.
INSERM U 313, Groupe Hospitalier Pitié-Salpêtrière, Paris, France.
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L
Rénia
D
D
Mattei
J
J
Goma
S
S
Pied
P
P
Dubois
F
F
Miltgen
A
A
Nüssler
H
H
Matile
F
F
Menégaux
M
M
Gentilini
D000818:Animals; D000911:Antibodies, Monoclonal; D000920:Antibody-Dependent Cell Cytotoxicity / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008099:Liver / Q000276:immunology* / Q000469:parasitology; D008288:Malaria / Q000276:immunology*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010963:Plasmodium falciparum / Q000276:immunology; D010967:Plasmodium yoelii / Q000276:immunology; D012377:Rodentia
D000911:Antibodies, Monoclonal; D006360:Heat-Shock Proteins