Establishment of a human T-acute lymphoblastic leukemia cell line with a (16;20) chromosome translocation.
A new T-cell line, Loucy, was established from the peripheral blood of a patient with T-cell acute lymphoblastic leukemia (T-ALL). The surface marker analysis of the cell line is OKT3+, OKT4+, THB4+, J5 +/-, OKT6-, TdT-, and HLA-DR-, indicating stage IV in T-cell lineage. Karyotype analysis revealed 45,X,5q-,t(16;20)(p12;q13). The translocation between chromosomes 16 and 20 has not been previously detected in ALL. This cell line may be of value in evaluating the role of t(16;20) in the etiology of T-ALL.
Laboratory of Experimental Surgery, Hadassah University Hospital, Jerusalem, Israel.
H
H
Ben-Bassat
Z
Z
Shlomai
G
G
Kohn
M
M
Prokocimer
D000954:Antigens, Surface / Q000032:analysis; D002460:Cell Line; D002478:Cells, Cultured; D002872:Chromosome Deletion; D002885:Chromosomes, Human, Pair 16; D002890:Chromosomes, Human, Pair 20; D002895:Chromosomes, Human, Pair 5; D005455:Fluorescent Antibody Technique; D006801:Humans; D007621:Karyotyping; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000235:genetics* / Q000276:immunology; D014178:Translocation, Genetic; D014998:Y Chromosome
D000954:Antigens, Surface