1-Methyladenine can consistently induce a fura-detectable transient calcium increase which is neither necessary nor sufficient for maturation in oocytes of the starfish Asterina miniata.
In starfish oocytes a calcium transient can consistently be detected with the dye fura-2 in response to perfusion of threshold levels of 1-methyladenine, the hormone responsible for induction of meiotic maturation. The calcium transient cannot be detected when the hormone is allowed to slowly diffuse to the oocyte, and the hormone-induced calcium transient can be inhibited by preperfusion of the oocyte with subthreshold levels of hormone. The calcium transient is shown to be unnecessary for maturation by eliciting maturation in situations that are not associated with a calcium transient, and the calcium transient is shown to be insufficient for maturation by eliciting a calcium transient and washing the hormone off the oocytes before the end of the hormone-dependent period. A mechanism is suggested to explain why some investigators have detected transient calcium rises during induction of oocyte maturation while others have not.
Department of Molecular and Cellular Biology, University of California, Berkeley 94720.
H J
HJ
Witchel
R A
RA
Steinhardt
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000502:physiology*; D004058:Diffusion; D005260:Female; D005306:Fertilization; D016257:Fura-2; D007700:Kinetics; D008540:Meiosis; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D013215:Starfish
C008407:1-methyladenine; D000225:Adenine; D002118:Calcium; D016257:Fura-2