Identification of 17-hydroxyprogesterone and other steroid hormones in saliva from a normal child and patients with congenital adrenal hyperplasia by plasmaspray liquid chromatography/mass spectrometry.
A very sensitive, selective and simultaneous method for the analysis of salivary steroid hormones was examined by discharge-assisted thermospray liquid chromatography mass spectrometry (plasmaspray LC/MS). Plasmaspray LC/MS gave [M + H]+ or [MH - H2O]+ as a predominant ion in most of the steroids in this work and in some cases fragment ions were also observed. Eight salivary steroid hormones, pregnenolone, 17-hydroxypregnenolone, progesterone, 17-hydroxyprogesterone, 11-deoxycortisol, cortisol, aldosterone, and testosterone were identified within 10 minutes using the selected ion monitoring technique in conjunction with plasmaspray LC/MS. Progesterone, 17-hydroxyprogesterone, cortisol, aldosterone and testosterone were detected in 1 mL of saliva from a normal child and two patients with congenital adrenal hyperplasia.
Division of Biochemical Analysis, Juntendo University, School of Medicine, Tokyo, Japan.
N
N
Shindo
N
N
Yamauchi
K
K
Murayama
A
A
Fairbrother
S
S
Korlik
D019326:17-alpha-Hydroxyprogesterone; D000312:Adrenal Hyperplasia, Congenital / Q000378:metabolism*; D002648:Child; D002853:Chromatography, Liquid; D006801:Humans; D006908:Hydroxyprogesterones / Q000032:analysis*; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D012463:Saliva / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013256:Steroids / Q000032:analysis*
D006908:Hydroxyprogesterones; D007202:Indicators and Reagents; D013256:Steroids; D019326:17-alpha-Hydroxyprogesterone