Induction of haptotactic migration of melanoma cells by neutrophil activating protein/interleukin-8.
Natural or recombinant neutrophil activating cytokine (IL-8) induced migration across polycarbonate filters of human A 2058 melanoma cells. Anti-IL-8 antibodies blocked IL-8 induced melanoma cell migration. Checkerboard experiments revealed a gradient-dependent response of A2058 melanoma cells to IL-8. Filters exposed to IL-8 and washed supported melanoma cell migration, thus implying a haptotactic component in the response. The homologous polypeptide platelet factor 4 was inactive. The observation that IL-8 affects melanoma cells emphasizes the need for a comprehensive analysis of the spectrum of action of platelet factor 4-related peptides. The effect of the inflammatory cytokine IL-8 on melanoma cells may be relevant to augmented secondary localization of tumors at sites of inflammation.
Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
J M
JM
Wang
G
G
Taraboletti
K
K
Matsushima
J
J
Van Damme
A
A
Mantovani
D002630:Chemotactic Factors / Q000494:pharmacology*; D002633:Chemotaxis / Q000187:drug effects*; D006801:Humans; D016209:Interleukin-8; D007378:Interleukins / Q000494:pharmacology*; D008545:Melanoma / Q000473:pathology*; D010978:Platelet Factor 4 / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology
D002630:Chemotactic Factors; D016209:Interleukin-8; D007378:Interleukins; D011994:Recombinant Proteins; D010978:Platelet Factor 4