Alterations to the penicillin-binding proteins in the Bacteroides fragilis group: a mechanism for non-beta-lactamase mediated cefoxitin resistance.
The penicillin-binding proteins (PBPs) of ATTC Type Strains of nine species of the Bacteroides fragilis group were visualized by gel electrophoresis and subsequent fluorography. Each species had a distinctive PBP pattern, although variation within species was seen. Generally, five PBPs could be visualized, ranging in molecular weight from approximately 40,000 to approximately 90,000. A laboratory-derived cefoxitin-resistant mutant of B. distasonis was compared with its wild type parent and cefoxitin-sensitive revertant. The fluorograph of the resistant mutant indicated a marked reduction of labelling to the PBP-1 complex as compared with the wild type and revertant. Cefoxitin-resistant clinical isolates of B. thetaiotaomicron and B. uniformis also showed changes to the PBP-1 complex, in comparison with sensitive strains.
Infectious Disease Section, VA Wadsworth Medical Center, Los Angeles, California 90073.
H M
HM
Wexler
S
S
Halebian
D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D001426:Bacterial Proteins; D001439:Bacteroides / Q000187:drug effects / Q000378:metabolism; D001441:Bacteroides fragilis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002440:Cefoxitin / Q000494:pharmacology*; D003470:Culture Media; D004352:Drug Resistance, Microbial; D004591:Electrophoresis, Polyacrylamide Gel; D006602:Hexosyltransferases; D008826:Microbial Sensitivity Tests; D002267:Muramoylpentapeptide Carboxypeptidase / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009608:Nitrous Acid / Q000633:toxicity; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D001618:beta-Lactamases / Q000032:analysis / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D003470:Culture Media; D046915:Penicillin-Binding Proteins; D002440:Cefoxitin; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; D001618:beta-Lactamases; D009608:Nitrous Acid