5-[[(carboxymethyl)amino]methyl]uridine is found in the anticodon of yeast mitochondrial tRNAs recognizing two-codon families ending in a purine.
The modified nucleoside (U*) present in the wobble position of Saccharomyces cerevisiae mitochondrial tRNA(Leu) and tRNA(Trp) was isolated by thin-layer chromatography and HPLC. Its chromatographic, UV spectral, and mass spectrometric properties were shown to be identical with those of 5- [[(carboxymethyl)amino]methyl]uridine (cmnm5U). This nucleoside found in yeast mitochondrial tRNAs reading two-codon families ending in a purine permits the selective recognition of A and G in the third codon position.
Martin
R P
RP
Institut de Biologie Moléculaire et Cellulaire du CNRS, Strasbourg, France.
Sibler
A P
AP
Gehrke
C W
CW
Kuo
K
K
Edmonds
C G
CG
McCloskey
J A
JA
Dirheimer
G
G
D000926:Anticodon / Q000235:genetics*; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry / Q000379:methods; D008928:Mitochondria / Q000502:physiology; D015394:Molecular Structure; D011687:Purines; D012343:RNA, Transfer / Q000235:genetics*; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012356:RNA, Transfer, Leu / Q000235:genetics*; D012364:RNA, Transfer, Trp / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000648:ultrastructure; D013056:Spectrophotometry, Ultraviolet; D014529:Uridine / Q000031:analogs & derivatives* / Q000235:genetics / Q000302:isolation & purification
D000926:Anticodon; D011687:Purines; D012345:RNA, Transfer, Amino Acid-Specific; D012356:RNA, Transfer, Leu; D012364:RNA, Transfer, Trp; C064149:5-(((carboxymethyl)amino)methyl)uridine; D012343:RNA, Transfer; C030985:purine; D014529:Uridine