Effect of topical retinoic acid on the interleukin 1 alpha and beta immunoreactive pool in normal human epidermis.
The topical application of 0.1% retinoic acid (RA) on human skin over a period of 4 days, whether or not under occlusion, did not increase either IL-1 alpha or beta immunoreactivity as determined by a sensitive enzymoimmunoassay. No down modulation was seen following the application of a potent topical corticosteroid. Occlusion increased the yield of IL-1 beta immunoreactivity. Immunoblot patterns of epidermal extracts revealed both the mature form of IL-1 (17 kDa) and the precursor (36 kDa) and were identical in amounts whether the specimens were from controls or from RA- or corticosteroid-treated skin. There was a slight modification in the pattern of high molecular weight proteins (52 kDa) probed by the anti-IL-1 alpha and beta sera. It appears that the IL-1 epidermal immunoreactive pools are barely amenable to modulation because they represent a storage form linked to end-stages of keratinocyte differentiation.
Department of Dermatology, University Hospital, Geneva, Switzerland.
D C
DC
Gruaz
L
L
Didierjean
D
D
Gumowski-Sunek
J H
JH
Saurat
D000287:Administration, Topical; D000293:Adolescent; D000328:Adult; D004817:Epidermis / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007375:Interleukin-1 / Q000378:metabolism*; D008875:Middle Aged; D011498:Protein Precursors / Q000378:metabolism; D014212:Tretinoin / Q000008:administration & dosage / Q000494:pharmacology*
D007375:Interleukin-1; D011498:Protein Precursors; C043830:interleukin 1 precursor; D014212:Tretinoin