Quantitative analysis of the selective pressure exerted on homologous proteins.
Evolution analysis is used to locate the regions of a protein that are important for its function or structure. The rate of evolution is generally constant for a given family of homologous sequences. From the starting point of this observation, an algorithm is proposed to establish quantitatively the sequence zones where selective pressure is maximal. A program that computes this pressure has been written in PASCAL. Analysis of results on some sequences validate this theoretical approach, and this knowledge can be used as a starting-point for carrying out site-directed mutagenesis.
CRBM du CNRS, U-249 INSERM, Montpellier, France.
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Blanca
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Ferraz
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Sri Widada
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JP
Liautard
D000465:Algorithms; D000595:Amino Acid Sequence; D000818:Animals; D003285:Contractile Proteins / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008840:Microfilament Proteins / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D051304:Profilins; D011381:Programming Languages; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D012641:Selection, Genetic; D012689:Sequence Homology, Nucleic Acid; D012984:Software; D013045:Species Specificity; D014335:Tropomyosin / Q000737:chemistry / Q000235:genetics
D003285:Contractile Proteins; D008840:Microfilament Proteins; C495738:PFN1 protein, human; D051304:Profilins; D011506:Proteins; D014335:Tropomyosin