[A joint study of mutation of the Ki-ras oncogene and overexpression of the Tp53 oncogene in colorectal cancer].
Twenty-five colorectal tumors (rectum 6, left colon 13, right colon 6) were studied with respect to the overexpression of p53 and the activation by point mutation of the Ki-ras oncogene. Single point mutations on codon 12 and codon 13 were analyzed after PCR amplivication, dotblotting and sequential hybridization with 12 different oligonucleotides. The intranuclear concentration of p53 protein was measured by flow cytometry after immunofluorescence staining with monoclonal antibody Pab 421. Twelve tumors were found to significantly overexpress p53 and 6 of them had an activated Ki-ras (5 on codon 12, 1 on codon 13). Of 13 tumors which failed to demonstrate over expression of p53, 8 had an activated Ki-ras (5 on codon 12, 3 on codon 13). In our series, p53 overexpression and ki-ras activation appeared to be independent.
Unité CNRS 620, Institut Curie, Paris.
P
P
Laurent-Puig
O
O
Delattre
S
S
Olschwang
Y
Y
Remvikos
T
T
Melot
R J
RJ
Salmon
G
G
Frelat
G
G
Thomas
D000230:Adenocarcinoma / Q000235:genetics*; D003062:Codon / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D011905:Genes, ras; D006801:Humans; D008297:Male; D009154:Mutation; D009857:Oncogenes; D016133:Polymerase Chain Reaction
D003062:Codon
Etude conjointe des mutations de l'oncogène Ki-ras et de la surexpression de l'oncogène Tp53 dans des cancers colorectaux.