Production of endothelin-1 by rat cultured mesangial cells.
We investigated whether ET-1 is synthesized by and released from mesangial cells. ET-1-like immunoreactivity (LI) released into medium increased time-dependently under a serum-free condition. The amounts of ET-1-LI released into the medium was augmented in the presence of fetal calf serum. Reverse-phase HPLC profile of the conditioned media revealed a major component coeluting with standard ET-1. Northern blot analysis of poly(A) +RNA extracted from mesangial cells showed a single major band corresponding to the size of preproET-1 mRNA (2.3 kb). These findings demonstrate that ET-1 is synthesized by and released from rat mesangial cells and suggest a possibility that it acts on their own cells as an autocrine factor.
Second Department of Internal Medicine, Tokyo Medical and Dental University, Yushima, Tokyo, Japan.
H
H
Sakamoto
S
S
Sasaki
Y
Y
Hirata
T
T
Imai
K
K
Ando
T
T
Ida
T
T
Sakurai
M
M
Yanagisawa
T
T
Masaki
F
F
Marumo
D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D016232:Endothelins; D004730:Endothelium, Vascular; D005920:Glomerular Mesangium / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D010452:Peptide Biosynthesis; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D016232:Endothelins; D010455:Peptides; D011498:Protein Precursors; D012333:RNA, Messenger