Gas chromatographic-electron-impact mass fragmentometric determination of lysergic acid diethylamide in urine.
A sensitive method for the detection and quantitation of lysergic acid diethylamide (LSD) in urine was developed. After initial solvent extraction, the compound was further purified by liquid-liquid extraction or by solid-phase extraction. The trimethylsilyl derivative of LSD was detected by gas chromatography-mass spectrometry (GC-MS) operated in the electron-impact mode with selected-ion monitoring. The presence of LSD was confirmed by comparing retention times and relative abundances of ions of unknowns with that of a standard. The recovery of this procedure was greater than 89%. The intra-run and inter-run coefficients of variation were less than 5% and less than 7%, respectively. This procedure allows detection of LSD concentrations as low as 29 pg/ml. Quantitation of LSD was linear over the concentration range 50-2000 pg/ml.
Navy Drug Screening Laboratory, Norfolk, VA 23511-6295.
B D
BD
Paul
J M
JM
Mitchell
R
R
Burbage
M
M
Moy
R
R
Sroka
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008238:Lysergic Acid Diethylamide / Q000652:urine*
D008238:Lysergic Acid Diethylamide