Computer-assisted analysis of chromosomal locations and transcriptional directions of Escherichia coli genes.
We present a computer-assisted method for locating and orienting nucleotide sequence segments on a large restriction map by comparing restriction fragment lengths. This method is based on the observation that long restriction fragments are rare and, therefore, the longest restriction fragments serve as effective discriminators. The method was applied to Escherichia coli genes, and chromosomal locations and transcriptional directions for more than 500 genes were determined.
Watanabe
H
H
Japan International Protein Information Database, Department of Applied Biological Science, Science University of Tokyo, Noda, Japan.
Kunisawa
T
T
D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003198:Computer Simulation; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D015183:Restriction Mapping; D014158:Transcription, Genetic