Production of recombinant human tropoelastin: characterization and demonstration of immunologic and chemotactic activity.
Tropoelastin cannot readily be prepared in quantity from natural sources and this has limited research in several important areas including structure/function relationships and fiber assembly. In order to eliminate this limitation, human tropoelastin has been expressed in a recombinant bacterial system and the protein has been highly purified. The size, amino acid composition, and sequence of the amino terminus of the recombinant tropoelastin (rTE) all agree with values predicted by the nucleotide sequence of the cDNA used in the expression vector. The rTE exhibits cross-reactivity with antibodies directed against a mixture of peptides derived from human elastin as well as antibody against a defined peptide located at the carboxy terminus of the protein. In addition, the rTE is chemotactic for fetal calf ligament fibroblasts. These results suggest that rTE could be a useful reagent for many types of studies.
Department of Anatomy and Histology, School of Dental Medicine, University of Pennsylvania, Philadelphia 19104.
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Indik
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Abrams
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Kucich
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Gibson
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Mecham
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Rosenbloom
D000818:Animals; D000906:Antibodies; D001483:Base Sequence; D002417:Cattle; D002633:Chemotaxis / Q000187:drug effects*; D003001:Cloning, Molecular; D003429:Cross Reactions; D004549:Elastin / Q000031:analogs & derivatives* / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000494:pharmacology; D015183:Restriction Mapping; D014333:Tropoelastin / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology
D000906:Antibodies; D011994:Recombinant Proteins; D014333:Tropoelastin; D004549:Elastin