Effect of erythroid differentiation factor on erythroid differentiation and proliferation of K-562 cells.
The effects of erythroid differentiation factor (EDF) on the levels of zeta-globin and several proto-oncogene mRNAs and transferrin receptors (Tf-R) of K-562 cells were examined. EDF decreased Tf-R expression and increased the level of zeta-globin mRNA. The mRNA level of c-fos began to rise within 3 hours and continued to increase up to 72 hours, but the levels of c-myb and c-abl decreased to 23 and 19%, respectively, of their initial levels after 48 hours. In contrast, the mRNA levels of c-myc and c-fms decreased transiently, but recovered within 48 hours. The modulation of several proto-oncogene mRNA levels was observed much earlier than the differentiation induction and the growth inhibition of K-562 cells by EDF.
First Department of Internal Medicine, School of Medicine, University of Tokushima, Japan.
Y
Y
Miyamoto
M
M
Kosaka
Y
Y
Eto
H
H
Shibai
S
S
Saito
D028341:Activins; D000911:Antibodies, Monoclonal; D015152:Blotting, Northern; D002454:Cell Differentiation; D002455:Cell Division; D004920:Erythropoiesis; D005434:Flow Cytometry; D005914:Globins / Q000235:genetics / Q000378:metabolism; D006427:Hemin / Q000494:pharmacology; D006801:Humans; D007265:Inhibins / Q000502:physiology*; D008937:Mitomycins / Q000494:pharmacology; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D011990:Receptors, Transferrin / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; C000715470:MAS1 protein, human; D008937:Mitomycins; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D011990:Receptors, Transferrin; D028341:Activins; D007265:Inhibins; D006427:Hemin; D005914:Globins