Elongation factor 1 beta of artemia: localization of functional sites and homology to elongation factor 1 delta.
Elongation factor (EF)-1 beta, a 26 kDa protein, is the eukaryotic equivalent of bacterial EF-Ts, the nucleotide exchange factor in protein synthesis. EF-1 beta catalyzes the exchange of guanine nucleotides bound to EF-1 alpha; the latter protein is the eukaryotic equivalent of bacterial EF-Tu. Limited proteolytic cleavage studies on EF-1 beta lead to the following picture: the protein is composed of two domains, an aminoterminal and a carboxyterminal domain, connected to each other by a stretch of hydrophilic, charged amino acids situated in the middle of the molecule. The carboxyterminal domain supplies the catalytic site for the nucleotide exchange reaction, whereas the aminoterminal domain interacts with EF-1 gamma, the third component of elongation factor 1. The regulatory, serine phosphate residue, Ser-89, localized in the hydrophilic stretch of EF-1 beta, does not appear to be necessary for the basic exchange reaction. The fourth component of the high molecular weight elongation factor complex (EF-1H), named EF-1 delta or 28 K protein, is homologous to EF-1 beta and contains regions very similar to the carboxyterminal part. EF-1 delta was found to be active in the nucleotide exchange reaction.
Department of Medical Biochemistry, Sylvius Laboratory, State University of Leiden, The Netherlands.
H T
HT
van Damme
R
R
Amons
R
R
Karssies
C J
CJ
Timmers
G M
GM
Janssen
W
W
Möller
D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia; D003546:Cysteine Endopeptidases / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D010206:Papain / Q000378:metabolism; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000235:genetics* / Q000378:metabolism*; D010446:Peptide Fragments / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid
D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D010446:Peptide Fragments; D003546:Cysteine Endopeptidases; D010206:Papain; C019913:clostripain