Generation and characterization of monoclonal antibodies to prostate secretory protein.
Monoclonal antibodies (MAbs) were produced against a highly purified preparation of prostate secretory protein (PSP) isolated from normal seminal plasma. Fifteen antibodies were selected for further evaluation based on their strong reactivity and specificity for PSP. All the MAbs had a specificity for prostate epithelial cells and none reacted to any of a variety of normal tissues as determined by immunoperoxidase staining. Six of the MAbs were selected for further immunohistochemical evaluation based on their ability to recognize different antigenic determinants. Using competitive binding immunoassays, a variety of overlapping specificities were observed with at least 2 distinct epitopes identified. Although some staining variability was noted, the 6 antibodies, in general, gave the same pattern of tissue reactivity. Both the normal prostate and the benign prostate hyperplastic ductal epithelial cells stained intensely, with 78 to 100% and 50-100% of the cells staining, respectively. The number and often the staining intensity of the tumor cells decreased as the tumor became more undifferentiated. Approximately 40 to 100% and 15 to 70% of the tumor cells stained in the moderately-differentiated and well-differentiated carcinoma tissues, respectively, whereas either no staining was observed or less than 20% of the tumor cells stained in the poorly-differentiated and undifferentiated tumors. Most of the metastatic prostate tumors showed either no staining or scattered staining in a few cells (i.e., less than 20%).
Department of Microbiology and Immunology, Eastern Virginia Medical School, Norfolk 23501.
G L
GL
Wright
C L
CL
Huang
G
G
Lipford
M L
ML
Beckett
H M
HM
Liang
C
C
Haley
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K
Newhall
M
M
Morningstar
D000230:Adenocarcinoma / Q000276:immunology; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D000918:Antibody Specificity / Q000276:immunology; D001667:Binding, Competitive; D001681:Biological Assay; D015153:Blotting, Western; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007124:Immunoenzyme Techniques; D007265:Inhibins; D008297:Male; D009363:Neoplasm Proteins / Q000276:immunology; D011467:Prostate / Q000276:immunology*; D011471:Prostatic Neoplasms / Q000276:immunology; D029584:Prostatic Secretory Proteins
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D009363:Neoplasm Proteins; D029584:Prostatic Secretory Proteins; C028321:estramustine-binding protein; D007265:Inhibins