[A new monoclonal antibody against human tubular brush border: preparation and its application in membranous nephropathy].
A new monoclonal antibody (McAb) against human tubular brush border was successfully prepared by authors using the hybridoma technique. The McAb produced was identified to be isotype of IgG1 by IF and SDS-PAGE assay. It was unique for its purity and specificity. To demonstrate the clinical usefulness, the McAb was applied for immunopathological study in 94 cases of renal biopsy of glomerular diseases. This McAb was distinguished by its specific deposition along the human tubular brush border and the subepithelial region in certain type of glomerular disease, particularly the MGN. Identification of this McAb might contribute to the immunopathological diagnosis of GN.
Chen
X M
XM
Department of Nephrology, Chinese PLA General Hospital, Beijing.
Yu
L F
LF
Tian
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D015433:Glomerulonephritis, Membranous / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007678:Kidney Glomerulus / Q000737:chemistry; D007684:Kidney Tubules / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal