Enhancement of dimethylnitrosamine-induced glutathione S-transferase P-positive hepatic foci by Clonorchis sinensis infestation in F344 rats.
The modifying potential of Clonorchis sinensis (CS) infestation on dimethylnitrosamine (DMN)-induced hepatocarcinogenesis was studied in male F344 rats. The metacercariae of CS were infected, 60 to each animal, by a single intragastric intubation at various times, before, during or after an 8-week period of DMN (25 ppm in drinking water) treatment. Controls were treated with DMN alone or infected with CS without carcinogen. Animals killed at the end of the 40-week experimental period showed a significant enhancing effect of CS on GST-P+ foci induction when CS was infected 4 weeks before DMN treatment, although no such influence was evident with CS infection during or following DMN exposure. The present findings suggest that CS might facilitate the proliferation of DMN-induced preneoplastic lesions of liver in rats.
Jang
J J
JJ
Department of Anatomical Pathology, Korea Cancer Center Hospital, Seoul.
Cho
K J
KJ
Myong
N H
NH
Chai
J Y
JY
D000818:Animals; D003003:Clonorchiasis / Q000150:complications*; D003004:Clonorchis sinensis; D004128:Dimethylnitrosamine; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000469:parasitology* / Q000473:pathology; D008297:Male; D009929:Organ Size; D011230:Precancerous Conditions / Q000473:pathology / Q000503:physiopathology*; D051381:Rats; D011916:Rats, Inbred F344; D013997:Time Factors
D005982:Glutathione Transferase; D004128:Dimethylnitrosamine