The sec and prl genes of Escherichia coli.
Two general approaches have been used to define genetically the genes that encode components of the cellular protein export machinery. One of these strategies identifies mutations that confer a conditional-lethal, pleiotropic export defect (sec, secretion). The other identifies dominant suppressors of signal sequence mutations (prl, protein localization). Subsequent characterization reveals that in at least three cases, prlA/secY, prlD/secA, and prlG/secE, both types of mutations are found within the same structural gene. This convergence is satisfying and provides compelling evidence for direct involvement of these gene products in the export process.
Bieker
K L
KL
Department of Biology, Princeton University, New Jersey 08544.
Phillips
G J
GJ
Silhavy
T J
TJ
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D021382:Protein Sorting Signals / Q000235:genetics*; D013489:Suppression, Genetic
D001426:Bacterial Proteins; D021382:Protein Sorting Signals