Determination of benzydamine and its N-oxide in biological fluids by high-performance liquid chromatography.
A simple, sensitive and selective method for the determination of benzydamine in human plasma and urine, and for benzydamine N-oxide in urine, has been developed using high-performance liquid chromatography in the reversed-phase mode. The limit of reliable determination of benzydamine in plasma was 0.5 ng/ml and that in urine 1 ng/ml; the limit of reliable determination of benzydamine N-oxide in urine was 50 ng/ml. The method has been successfully applied to the analysis of these compounds in biological fluids after administration of intravenous and oral doses of benzydamine to human volunteers.
Department of Metabolism and Pharmacokinetics, Huntingdon Research Centre, Cambridgeshire, U.K.
G A
GA
Baldock
R R
RR
Brodie
L F
LF
Chasseaud
T
T
Taylor
D001591:Benzydamine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D006801:Humans
C048872:benzydamine N-oxide; D001591:Benzydamine