[Comparative study of bovine adrenodoxin and renorenodoxin].
The ferredoxin from bovine renal mitochondria (renoredoxin) has been obtained in a highly purified state. The A415/A280 ratio of the purified renoredoxin is 0.84. The absorption spectrum of renoredoxin was shown to be identical to that of bovine adrenodoxin. Two forms of renoredoxin (Mr 14200 and 13300) were detected by using polyacrylamide gel electrophoresis. These forms exhibit a very similar immunologic cross-reactivity with polyclonal antibodies to adrenodoxin. The N-terminal amino acid sequence of renal ferredoxin was shown to be identical to that of adrenodoxin; the C-terminal sequences of both ferredoxins undergo a similar post-translational proteolytic modification. The amino acid composition of ferredoxins are also very close. Renal ferredoxin can be replaced by adrenodoxin in reconstituted systems from bovine adrenal cortex mitochondria which catalyze the side chain cleavage of cholesterol to pregnenolone and the 11 beta-hydroxylation of deoxycorticosterone to corticosterone.
Lobanov
N A
NA
Vlasova
T M
TM
Adamovich
T B
TB
D000325:Adrenodoxin / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D003345:Corticosterone / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D005288:Ferredoxins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D015151:Immunoblotting; D007668:Kidney / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism; D008928:Mitochondria / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D011284:Pregnenolone / Q000096:biosynthesis; D013252:Steroid 11-beta-Hydroxylase / Q000378:metabolism
D005288:Ferredoxins; C050867:renoredoxin; D000325:Adrenodoxin; D011284:Pregnenolone; D013252:Steroid 11-beta-Hydroxylase; D002786:Cholesterol Side-Chain Cleavage Enzyme; D003345:Corticosterone
Sravnitel'noe izuchenie adrenodoksina i renoredoksina byka.