Enzyme synthesis of L-tryptophan.
Enzyme synthesis of tryptophan from indole, pyruvate and ammonium salts was studied using Escherichia coli cells exhibiting a significant tryptophanase activity. In addition to the effect of cultivation medium composition and cultivation conditions, factors affecting the course of the conversion were investigated. Production of 32.4 g/L of L-tryptophan was reached after 48 h under optimal conditions.
Research Institute of Antibiotics and Biotransformations, Roztoky near Prague, Czechoslovakia.
R
R
Zeman
J
J
Plachý
H
H
Bulantová
B
B
Sikyta
E
E
Pavlasová
E
E
Stejskalová
D000085:Acetates / Q000378:metabolism; D000332:Aerobiosis; D004926:Escherichia coli / Q000201:enzymology*; D007211:Indoles / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D014364:Tryptophan / Q000096:biosynthesis*; D014368:Tryptophanase / Q000378:metabolism*
D000085:Acetates; D007211:Indoles; D011773:Pyruvates; D014364:Tryptophan; D014368:Tryptophanase; C018824:ammonium acetate