A "treated" model for severe hemorrhagic shock: a comparison of conventional and germ-free animals.
In an unanesthetized "treated" model of severe hemorrhagic shock, a bacteremia originating from the animal's enteric flora was demonstrated by finding radiolabeled Escherichia coli in the blood as early as 2 hr after the onset of shock. In 50 patients admitted to our trauma unit, the observation was similar, with 56% positive blood cultures, when their mean arterial blood pressure was 80 mmHg or less. The relationship of bacterial translocation and the high post-shock mortality in our conventional (CV) animal model is being evaluated in germfree (GF) Sprague-Dawley rats. Preliminary observations in 15 GF animals showed 80% survival at 24 hr post-shock, 54% at 48 hr, and 42% at 72 hr compared with those of CV animals in previous experiments with survival at similar times of 73%, 20% and 7%, respectively.
Flanagan
J J
JJ
Department of Surgery, UMD-New Jersey Medical School, Newark 07103.
Rush
B F
BF
Murphy
T F
TF
Smith
S
S
Machiedo
G W
GW
Hsieh
J
J
Rosa
D M
DM
Heneghan
J B
JB
D000818:Animals; D001424:Bacterial Infections / Q000209:etiology; D001769:Blood / Q000382:microbiology; D001794:Blood Pressure; D001831:Body Temperature; D002440:Cefoxitin / Q000494:pharmacology; D004285:Dogs; D005440:Fluid Therapy; D005856:Germ-Free Life; D007422:Intestines / Q000382:microbiology; D012016:Reference Values; D012771:Shock, Hemorrhagic / Q000401:mortality / Q000503:physiopathology / Q000628:therapy*; D016019:Survival Analysis; D014947:Wounds and Injuries / Q000150:complications
D002440:Cefoxitin