Epidermal growth factor receptors in colorectal carcinoma.
Nineteen samples of primary colorectal carcinoma and adjacent mucosa were examined for EGFr expression using radioligand binding assays and immunohistochemical staining with the monoclonal antibody EGFR1. Radioligand binding experiments showed expression of EGFr in both tumour and mucosa in all cases. In tumour samples EGFr levels ranged between 4 and 79 fmole per mg membrane protein (Kd = 0.1-0.4 X 10(-9) M). There was no significant difference in the level of EGFr expression between tumour and mucosa overall. Immunohistochemical staining with the EGFR1 antibody was useful in localising EGFr to epithelial elements although it was less sensitive than ligand binding assays.
Department of Pathology, University of Newcastle Upon Tyne, U.K.
M
M
Moorghen
P
P
Ince
K J
KJ
Finney
A J
AJ
Watson
A L
AL
Harris
D000230:Adenocarcinoma / Q000473:pathology; D000911:Antibodies, Monoclonal; D002462:Cell Membrane / Q000378:metabolism; D003110:Colonic Neoplasms / Q000473:pathology*; D004815:Epidermal Growth Factor / Q000378:metabolism; D066246:ErbB Receptors / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D007413:Intestinal Mucosa / Q000473:pathology; D007700:Kinetics; D011869:Radioligand Assay; D012004:Rectal Neoplasms / Q000473:pathology*; D013997:Time Factors
D000911:Antibodies, Monoclonal; D004815:Epidermal Growth Factor; D066246:ErbB Receptors