Simultaneous determination of amino-alpha-carbolines and amino-gamma-carbolines in cigarette smoke condensate by high-performance liquid chromatography.
A method for the simultaneous detection of amino-alpha-carbolines (2-amino-alpha-carboline and 2-amino-3-methyl-alpha-carboline) and amino-gamma-carbolines (3-amino-1,4-dimethyl-5H-pyrido [4,3-b]indole and 3-amino-1-methyl-5H-pyrido [4,3-b]indole) by high-performance liquid chromatography has been developed. It consists of a three-step purification using three different columns with fluorometric detection. With this method, we have demonstrated that both amino-alpha-carbolines and amino-gamma-carbolines are present in cigarette smoke condensate. The method may be useful for detecting these carcinogens in various materials.
Department of Hygiene and Preventive Medicine, Faculty of Medicine, University of Tokyo, Japan.
S
S
Manabe
O
O
Wada
Y
Y
Kanai
D002243:Carbolines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D010947:Plants, Toxic; D012906:Smoke / Q000032:analysis*; D014026:Nicotiana / Q000032:analysis*
D002243:Carbolines; D012906:Smoke