[The possibility of the incorporation of macromolecules, including exogenous DNA, into the germ cells of male mice. The liposome method and Ca-P coprecipitation method].
Liposomes loaded with FITC-labeled albumin in the presence of PEG-1,500 are actively sorbed on the membranes of mature spermatozoa and remain attached even after thorough washing. Immature sperm cells are able to incorporate alien DNA carried by liposomes. In contrast, the mature spermatozoa could not incorporate plasmid DNA loaded with positively charged liposomes. Chlortetracycline in Ca-P coprecipitate crystals is tightly fixed in the postacrosomal region of mature sperms. Intensity of staining of chlortetracycline is stimulated by DNA load in Ca-P coprecipitate as well as by DMSO or EDTA. The method of Ca-P coprecipitation could not provide for plasmid DNA incorporation into taure sperms. Foreign DNA incorporation in postacrosomal regions of sperm heads seems quite possible in experiments with dimethylsulphoxide (DMSO).
V S
VS
Baranov
B
B
Brobohatý
N V
NV
Tsymbalenko
L
L
Tádlicek
V N
VN
Gorbunova
T B
TB
Kazakova
L
L
Kovác
K
K
Bodja
D000818:Animals; D001345:Autoradiography; D002118:Calcium; D011232:Chemical Precipitation; D004247:DNA / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D004337:Drug Carriers; D005821:Genetic Techniques; D008081:Liposomes; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D010758:Phosphorus; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D004337:Drug Carriers; D008081:Liposomes; D046911:Macromolecular Substances; D010758:Phosphorus; D004247:DNA; D002118:Calcium
K vozmozhnosti inkorporirovaniia makromolekul, vkluchaia ékzogennuiu DNK, v polovye kletki samtsov mysheĭ. Liposomnyĭ metod i metod Ca-P kopretsipitatsii.