Endothelin-1 stimulates arachidonate 15-lipoxygenase activity and oxygen radical formation in the rat distal lung.
We investigated the effects of intravenous bolus of endothelin-1 on the metabolism of eicosanoids and oxygen radicals in the distal lung unit of the rat. Intravenous bolus of endothelin-1 caused a significant increase in 15-hydroxyeicosatetraenoic acid of bronchoalveolar lavage fluid and oxygen radicals produced by the bronchoalveolar cells. Endothelin-1 exhibited a stimulatory effect on the 15-lipoxygenase activity in the lung homogenate. Thus, endothelin-1 may contribute to the inflammatory and hyperreactive process of lungs, by enhancing the release of 15-hydroxyeicosatetraenoic acid and oxygen radicals in the distal lung unit.
Department of Geriatrics, Faculty of Medicine, University of Tokyo, Japan.
T
T
Nagase
Y
Y
Fukuchi
C
C
Jo
S
S
Teramoto
Y
Y
Uejima
K
K
Ishida
T
T
Shimizu
H
H
Orimo
D000818:Animals; D001093:Arachidonate 15-Lipoxygenase / Q000378:metabolism*; D001091:Arachidonate Lipoxygenases / Q000378:metabolism*; D001992:Bronchoalveolar Lavage Fluid; D004537:Eicosanoic Acids / Q000378:metabolism; D016232:Endothelins; D005260:Female; D005609:Free Radicals; D066298:In Vitro Techniques; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D004537:Eicosanoic Acids; D016232:Endothelins; D005609:Free Radicals; D010455:Peptides; D001091:Arachidonate Lipoxygenases; D001093:Arachidonate 15-Lipoxygenase; D010100:Oxygen