Detection of S-100b protein in Triton cytoskeletons: an immunocytochemical study on cultured Schwann cells.
We investigated the subcellular distribution of S-100b protein in primary cultures of Schwann cells. The subcellular localization of the protein in cells fixed and then permeabilized is similar, if not identical, to that seen in Schwann cells in peripheral nerves, i.e., S-100b protein is found in the cytoplasm and associated with membranes and filamentous structures. In cells either fixed in the presence of Triton X-100 or exposed to Triton X-100 for a short time before fixation (Triton cytoskeletons), the immune reaction product is considerably less intense, and the protein is associated with filaments running parallel to the long axis of the cell as well as in a submembranous position. Including CaCl2 in the buffer during fixation in the presence of Triton X-100 does not result in any increase in the intensity of the immune reaction product in Triton cytoskeletons, suggesting that, within the limits of the technique employed, no binding of additional S-100b protein to the Triton X-100-resistant material can be induced. On the other hand, including EGTA results in a substantial decrease in the intensity of the immune reaction product in Triton cytoskeletons. Altogether, these findings suggest that a remarkable fraction of S-100b protein in cultured Schwann cells is associated with elements of the cytoskeleton and that Ca2+ exerts some regulatory role in the association of S-100b protein with the cytoskeleton.
Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
M G
MG
Rambotti
A
A
Spreca
P
P
Leoncini
M
M
Estenoz
E
E
Costantino-Ceccarini
I
I
Giambanco
R
R
Donato
D000818:Animals; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D008854:Microscopy, Electron / Q000379:methods; D009414:Nerve Growth Factors; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000032:analysis / Q000378:metabolism*; D012583:Schwann Cells / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure
D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol