[Methods for studying the hepatic metabolism of drugs in man].
Various methods can be used to investigate human hepatic drug metabolism in vivo. a) Indirect methods based on the assessment of atoxic substances metabolism in order to investigate: --hepatic oxidative activity by antipyrine or caffeine clearance and aminopyrine breath test determination. Such an activity can be modified by drug-induced enzyme induction or inhibition and in case of hepatic disease; --genetic polymorphism of hepatic drug metabolism assessed by the determination of debrisoquine or dextrometorphane metabolic ratio and of N-acetylation phénotype. Unusual therapeutic or adverse effects could be explained by such a polymorphism; --hepatic blood flow, which variations could modify hepatic clearance of drugs with a high hepatic extraction ratio, by the assessment of indocyanine green clearance. b) Direct methods based on pharmacokinetics data and their alterations under various circumstances: simultaneous administration of other drugs, liver disease.
Geneve
J
J
Clinique Thérapeutique, Hôpital Lariboisière, Paris.
Bergmann
J F
JF
Caulin
C
C
Segrestaa
J M
JM
D006801:Humans; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008722:Methods; D010084:Oxidation-Reduction; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D010599:Pharmacokinetics; D011110:Polymorphism, Genetic
D004364:Pharmaceutical Preparations
Méthodes d'exploration du métabolisme hépatique des médicaments chez l'homme.