[Determination of the activity of alanine aminotransferase].
Methods currently recommended by French, European and International Societies for determining the catalytic concentration of Alanine Aminotransferase (ALT) are reported. These methods are very close, recommend the apoenzyme reactivation by pyridoxal phosphate and allow the transferability of the results from site to site. Complementary information concerning commercial kits, adaptation to enzyme analyzers, specimen collection and storage, reference values and biological variations in healthy subjects are also given. The adoption of these homogeneous methods should make easier the choice of a cutoff value for ALT among blood donors for the prevention of posttransfusion hepatitis.
Laboratoire d'Enzymologie, Hôpital Debrousse, Lyon.
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D000410:Alanine Transaminase / Q000097:blood*; D001051:Apoenzymes / Q000378:metabolism; D001782:Blood Donors; D001800:Blood Specimen Collection; D003951:Diagnostic Errors; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D006525:Hepatitis, Viral, Human / Q000097:blood / Q000517:prevention & control; D006801:Humans; D008111:Liver Function Tests / Q000379:methods; D008297:Male; D011732:Pyridoxal Phosphate / Q000494:pharmacology; D011933:Reagent Kits, Diagnostic; D012016:Reference Values
D001051:Apoenzymes; D011933:Reagent Kits, Diagnostic; D011732:Pyridoxal Phosphate; D000410:Alanine Transaminase
Détermination d'activité de l'alanine aminotransférase (ALT).