Specificity of DNA probes for the detection of toxigenic Pasteurella multocida subsp. multocida strains.
Five DNA probes directed against different regions of the gene that encodes the dermonecrotic toxin of Pasteurella multocida subsp. multocida were examined for their ability to identify toxigenic P. multocida subsp. multocida strains. The specificities of the probes were studied with 96 strains of P. multocida subsp. multocida and 22 strains of 11 other bacterial species. Results of colony hybridization assays using these probes indicated that two of the five probes have potential diagnostic value.
Department of Bacteriology, Central Veterinary Institute, Lelystad, The Netherlands.
A M
AM
Kamps
W E
WE
Buys
E M
EM
Kamp
M A
MA
Smits
D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000382:microbiology; D015342:DNA Probes; D003885:Dermotoxins / Q000235:genetics*; D005260:Female; D008297:Male; D010325:Pasteurella / Q000235:genetics*; D010326:Pasteurella Infections / Q000175:diagnosis / Q000662:veterinary; D015183:Restriction Mapping; D012222:Rhinitis, Atrophic / Q000175:diagnosis / Q000662:veterinary; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000382:microbiology
D001427:Bacterial Toxins; D015342:DNA Probes; D003885:Dermotoxins; C052804:dermonecrotic toxin, Pasteurella multocida