Influence of polyethylene glycol (PEG) extraction on the C-peptide determination in sera from insulin-dependent diabetic patients with circulating insulin antibodies.
To investigate whether the unexpectedly high C-peptide levels in some insulin-dependent diabetic (IDDM) patients are due to co-determination of proinsulin bound to circulating insulin antibodies, 36 randomly selected sera from IDDM patients were assayed for C-peptide immunoreactivity (CPR) after polyethylene glycol (PEG) extraction, preceding incubation with proinsulin binding antibodies (LAB + PEG) or without pretreatment of the sera. Recovery of proinsulin was checked by addition of 1 nmol/l proinsulin to all sera. Recovery was found to be 101.5 +/- 4.0%. The mean values of concentrations were significantly lower (p less than 0.001) after treatment with PEG and IAB + PEG compared to the untreated sera. There was also a significant difference (p less than 0.05) between sera extracted with PEG alone or after IAB + PEG-treatment. However, no correlation (p greater than 0.1) was found to bound insulin (total minus free insulin) or to insulin binding capacity (IBC) of the sera. If an antiserum is not available with very low cross-reactivity with proinsulin to determine human C-peptide then sera should not be extracted with PEG alone but after additional incubation with a proinsulin binding antiserum. In spite of the extraction in some cases unexplicably high C-peptide levels may still be expected.
Central Institute of Diabetes, Gerhardt Katsch, Karlsburg/GDR.
W
W
Besch
H
H
Keilacker
K D
KD
Kohnert
M
M
Ziegler
D002096:C-Peptide / Q000097:blood* / Q000276:immunology; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000276:immunology*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011384:Proinsulin / Q000276:immunology; D011863:Radioimmunoassay
D002096:C-Peptide; D007328:Insulin; D011092:Polyethylene Glycols; D011384:Proinsulin