Identification of enterotoxigenic Escherichia coli by colony hybridization with nonradioactive digoxigenin-labeled DNA probes.
Enterotoxigenic Escherichia coli (ETEC) strains were readily identified in pure and mixed cultures with nonradioactive, digoxigenin-labeled DNA probes coding for heat-labile (LTI) and heat-stable (STaI, STaII, and STb) enterotoxins. Digoxigenin-labeled ETEC fragments were more sensitive than and exhibited less nonspecific background contamination than biotinylated ETEC probes.
Riley
L K
LK
Department of Veterinary Pathology, University of Missouri, Columbia 65211.
Caffrey
C J
CJ
D000818:Animals; D001431:Bacteriological Techniques; D015342:DNA Probes; D004076:Digoxigenin; D004077:Digoxin / Q000031:analogs & derivatives; D004285:Dogs; D004768:Enterotoxins / Q000378:metabolism*; D004926:Escherichia coli / Q000302:isolation & purification* / Q000378:metabolism / Q000472:pathogenicity; D006801:Humans; D013552:Swine
D015342:DNA Probes; D004768:Enterotoxins; D004077:Digoxin; D004076:Digoxigenin