Expression of simian virus 40 large T antigen in Escherichia coli using vectors based on the regulatable rac promoter.
Simian Virus 40 large T antigen is a multi-functional protein that is involved in the initiation of viral DNA replication, regulation of viral transcription and cell transformation. Bacterial expression vectors, pER23-1 and pER23-2, that are based on the regulatable rac promoter were used to produce T antigen either as a free protein or as a fusion protein. We have observed efficient transcription of the cloned T antigen gene in most of the recombinants. However, expression of the T antigen protein was inefficient and most of the expressed protein was truncated. This may be due to differences in codon usage in E. coli or to rapid protein degradation.
Pistillo
J M
JM
Department of Biochemistry, University of Nebraska Medical Center, Omaha 68198-4525.
Vishwanatha
J K
JK
D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D003062:Codon; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics
D000952:Antigens, Polyomavirus Transforming; D003062:Codon; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins