A simple and rapid method to determine hematopoietic growth factor activity.
A rapid and simple colorimetric microassay method for the determination of hematopoietic growth factor activities was established. The assay was used to detect CSF-1, GM-CSF, and IL-3 activities. The assay was based on the metabolism of the tetrazolium dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide to formazan by metabolically active cells. Results obtained with the colorimetric microassay are comparable with those obtained with the soft agarose assay. Advantages of the colorimetric microassay include the conservation of reagents, the shorter incubation time for the experiment, the shorter assay time, and the ability to evaluate large numbers of samples.
Department of Microbiology, University of Texas Medical Branch, Galveston 77550.
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Kotnik
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Fleischmann
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000378:metabolism; D003124:Colorimetry / Q000379:methods*; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000378:metabolism*; D016298:Hematopoietic Cell Growth Factors; D007377:Interleukin-3 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins; D015203:Reproducibility of Results; D012685:Sepharose; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D016298:Hematopoietic Cell Growth Factors; D007377:Interleukin-3; D011994:Recombinant Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D012685:Sepharose; C022616:thiazolyl blue