Microenzyme immunoassay for the measurement of brain natriuretic peptide (BNP)-like immunoreactivity in porcine plasma.
A sensitive and specific microenzyme immunoassay (EIA) procedure for porcine brain natriuretic peptide (BNP)-like immunoreactivity has been developed. Enzyme-labeled antigen was prepared by conjugation of synthetic BNP with beta-D-galactosidase using N-(epsilon-maleimidocaproyloxy)succinimide method. Using a second antibody-coated immunoplate, the minimum amount of BNP-like immunoreactivity (BNP-LI) detectable by this assay system was 1.6 fmol/well. When porcine BNP-LI in porcine plasma was assayed by the present method levels between 1 and 8 pmol/l were detected. Gel filtration of porcine plasma extracts on Sephadex G-25 revealed the presence of two immunoreactive peaks; one eluted at a position identical with that of BNP-26 and the other eluted earlier, close the position of BNP-32.
Department of Hospital Pharmacy, Medical College of Oita, Japan.
M
M
Takeyama
F
F
Yasunaga
A
A
Otaka
N
N
Fujii
H
H
Yajima
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D002850:Chromatography, Gel; D006801:Humans; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D020097:Natriuretic Peptide, Brain; D009419:Nerve Tissue Proteins / Q000097:blood*; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013552:Swine
D009419:Nerve Tissue Proteins; D020097:Natriuretic Peptide, Brain