Effects of toxin T-544 from the Karwinskia humboldtiana (buckthorn) plant upon mouse embryos explanted at 11 days.
Eleven-day mouse embryos were exposed to the K. humboldtiana toxin T-544 for 24 hr. At the end of the culture period, embryos were examined grossly for malformations and biochemically for altered protein levels. There was a significant difference in malformations in those embryos exposed to 0.05 and 0.2 microgram/ml of toxin compared with controls. Embryo protein content was significantly lower in those embryos exposed to 0.1 microgram/ml of T-544 compared with control group.
Departamento de Farmacología y Toxicología, Facultad de Medicina, Universidad Autónoma de Nuevo León, México.
L
L
Martínez de Villarreal
R
R
Velazco-Campos
A
A
Piñeyro López
R
R
González Alanís
D000818:Animals; D004622:Embryo, Mammalian / Q000187:drug effects*; D005314:Embryonic and Fetal Development; D005638:Fruit / Q000633:toxicity*; D066298:In Vitro Techniques; D051379:Mice; D010936:Plant Extracts / Q000633:toxicity; D010946:Plants, Medicinal; D010947:Plants, Toxic; D031955:Rhamnus; D013723:Teratogens
D010936:Plant Extracts; D013723:Teratogens