Immediate elimination of labile HbA1c with allosteric effectors of hemoglobin.
Our aim was to find a simple method of removing labile glycosylated hemoglobin (HbA1c) from blood samples before it is measured by cation-exchange chromatography. Labile HbA1c is formed by the binding of glucose to the NH2-terminal valine of the beta-chain of HbA. We sought a more competitive binder for the same site to dissociate labile HbA1c to glucose and HbA. Inorganic phosphates were found to have a strong allosteric effect and a great ability to eliminate labile HbA1c. We developed our method with 4 mM tetrapolyphosphate in the hemolyzing solution to eliminate labile HbA1c during the automatic processing (at pH 6 and heated for 2 min at 45 degrees C) of blood samples for HbA1c estimation. This may be useful when estimating HbA1c by the manual method.
Department of Laboratory Medicine, St. Luke's College of Nursing, Tokyo, Japan.
K
K
Nakashima
Y
Y
Hattori
K
K
Yamazaki
M
M
Takechi
Y
Y
Andoh
T
T
Miyaji
D000494:Allosteric Regulation / Q000502:physiology; D000495:Allosteric Site; D002852:Chromatography, Ion Exchange; D004163:Diphosphoglyceric Acids / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D006442:Glycated Hemoglobin / Q000032:analysis* / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D010100:Oxygen / Q000378:metabolism; D010833:Phytic Acid / Q000494:pharmacology*; D011122:Polyphosphates / Q000494:pharmacology*
D004163:Diphosphoglyceric Acids; D005947:Glucose; D006442:Glycated Hemoglobin; D006454:Hemoglobins; D010100:Oxygen; D010833:Phytic Acid; D011122:Polyphosphates