A method to determine the end point of decalcification of hard tissue and bone.
A method for decalcification end point determination of mineralized tissue is described. The calcium content of the decalcification solution was determined colorimetrically with a "continuous automatic analyzer" with a high degree of accuracy. The end point method used has been tested on two decalcification methods, 5% nitric acid with or without ultrasonic treatment. The results suggest it is possible to quantitate the decalcification process.
Müller
S
S
Department of Stomatology, School of Medicine, Charité, Regional Hospital, Heinrich Braun, Zwickau, German Democratic Republic.
Pleul
J
J
Götz
M
M
Jähnig
J
J
Schädlich
H
H
D000328:Adult; D001842:Bone and Bones / Q000032:analysis*; D002118:Calcium / Q000032:analysis*; D003124:Colorimetry / Q000295:instrumentation* / Q000379:methods; D003648:Decalcification Technique; D006801:Humans; D014070:Tooth / Q000032:analysis*
D002118:Calcium