Electrophoretic karyotyping of typical and atypical Candida albicans.
Electrophoretic karyotypes of atypical isolates of Candida albicans, e.g., strains that were germ tube negative, failed to express proteinase activity, demonstrated low virulence for mice, formed hyperchlamydoconidia, produced hyperhyphae, or were sucrose negative (including the type strain of Candida stellatoidea), were compared with those of typical C. albicans. Karyotypes of whole-cell DNA of classical C. albicans examined with transverse alternating-field electrophoresis under specific conditions were composed of seven DNA bands with a specific migration pattern. Certain atypical strains and representatives of the three serotypes of C. stellatoidea produced discrete karyotypes with 5 to 10 bands. All isolates demonstrated a significant degree of DNA relatedness, suggesting their conspecificity. Densitometric tracings of DNA bands provided an objective and standardized method for comparing bands within the gels.
Laboratory for Microbial and Biochemical Sciences, Georgia State University, Atlanta 30303.
M
M
Mahrous
T J
TJ
Lott
S A
SA
Meyer
A D
AD
Sawant
D G
DG
Ahearn
D002176:Candida albicans / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007621:Karyotyping; D010641:Phenotype
D004271:DNA, Fungal