Isolation of a functional vesicular intermediate that mediates ER to Golgi transport in yeast.
We have used an in vitro assay that reconstitutes transport from the ER to the Golgi complex in yeast to identify a functional vesicular intermediate in transit to the Golgi apparatus. Permeabilized yeast cells, which serve as the donor in this assay, release a homogeneous population of vesicles that are biochemically distinct from the donor ER fraction. The isolated vesicles, containing a post-ER/pre-Golgi form of the marker protein pro-alpha-factor, were able to bind to and fuse with exogenously added Golgi membranes. The ability to isolate fusion competent vesicles provides direct evidence that ER to Golgi membrane transport is mediated by a discrete population of vesicular carriers.
Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510-8002.
M E
ME
Groesch
H
H
Ruohola
R
R
Bacon
G
G
Rossi
S
S
Ferro-Novick
D001692:Biological Transport; D003600:Cytosol / Q000378:metabolism / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000378:metabolism* / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D007700:Kinetics; D000072235:Mating Factor; D008954:Models, Biological; D008970:Molecular Weight; D015388:Organelles / Q000378:metabolism* / Q000648:ultrastructure; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D010675:Pheromones / Q000235:genetics; D011498:Protein Precursors / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism* / Q000648:ultrastructure
D010455:Peptides; D010675:Pheromones; D011498:Protein Precursors; D000072235:Mating Factor