[Replication characteristics of plasmids and their dependence of recA gene for the initiation of Escherichia coli chromosome replication in the integrated state].
E. coli dnaA46 fails to grow and form colonies at 40 degrees C; integrated with plasmid enables it to grow at 40 degrees C or 42 degrees C. In a previous paper we reported that the replication of chromosome initiated by the integrated F' plasmid was recA gene dependent. In this paper we report further the behavior of 5 plasmids and 2 phages as to their dependence of recA gene in the integrated state. It was demonstrated that the dependence or independence of recA gene is not related to the direction of replication of the plasmids or phages in the free state. The hypothesis that the function of recA gene is to convert the unidirectionally replicating machinery in the free state to the bidirectionally replicating one in the integrated state is refuted accordingly.
Mao
Y M
YM
Institute of Genetics, Fudan University, Shanghai.
Lu
J W
JW
Sheng
Z J
ZJ
D002876:Chromosomes, Bacterial / Q000378:metabolism*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D008242:Lysogeny; D010957:Plasmids / Q000235:genetics*
D004269:DNA, Bacterial