Isolation and characterization of Candida albicans morphological mutants derepressed for the formation of filamentous hypha-type structures.
Several Candida albicans morphological mutants were obtained by a procedure based on a combined treatment with nitrous acid plus UV irradiation and a double-enrichment step to increase the proportion of mutants growing as long filamentous structures. Altered cell morphogenesis in these mutants correlated with an altered colonial phenotype. Two of these mutants, C. albicans NEL102 and NEL103, were selected and characterized. Mutant blastoconidia initiated budding but eventually gave rise to filamentous hypha-type formations. These filaments were long and septate, and they branched very regularly at positions near septa. Calcofluor white (which is known to bind chitin-rich areas) stained septa, branching zones, and filament tips very intensely, as observed under the fluorescence microscope. Wild-type hybrids were obtained by fusing protoplasts of strain NEL102 with B14, another morphological mutant previously described as being permanently pseudomycelial, indicating that genetic determinants responsible for the two altered phenotypes are different. The mutants characterized in this work seemed to sequentially express the morphogenic characteristics of C. albicans, from blastoconidia to hyphae, in the absence of any inducer. Further characterization of these strains could be relevant to gain understanding of the genetic control of dimorphism in this species.
Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
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Pomés
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Nombela
D002176:Candida albicans / Q000166:cytology* / Q000235:genetics / Q000302:isolation & purification; D005816:Genetic Complementation Test; D008561:Membrane Fusion; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D008938:Mitosis / Q000528:radiation effects; D009154:Mutation; D011523:Protoplasts / Q000502:physiology; D014466:Ultraviolet Rays