Luteinizing hormone and human chorionic gonadotropin receptors in human corpora lutea from clomiphene citrate-induced cycles.
Midluteal phase corpora lutea (CL) obtained from women induced with 50 mg (n = 5), 100 mg (n = 5), and 150 mg (n = 5) of clomiphene citrate (CC) were measured for luteinizing hormone/human chorionic gonadotropin (LH/hCG) concentrations and cytosol progesterone (P) and 17 alpha-hydroxyprogesterone (17-OHP) and compared with midluteal phase CL from eight normal women (controls). More CL (26) that were significantly heavier (2.0 +/- 0.3 g, [mean +/- SEM]) were obtained with CC than in controls (10). Clomiphene citrate treatment increased LH/hCG receptor concentrations and the dissociation constant significantly from 69 +/- 12 fmol/mg protein and 1.1 +/- 0.2 x 10(-10) M, respectively, in controls to 112 +/- 6 fmol/mg protein and 2.1 +/- 0.1 X 10(-10) M. Cytosol P and 17-OHP levels were not significantly increased. Cumulatively these cellular effects may be responsible for increasing serum P and responsiveness to hCG and for correcting luteal dysfunction.
Yeko
T R
TR
University of Illinois College of Medicine, Chicago.
Khan-Dawood
F S
FS
Dawood
M Y
MY
D019326:17-alpha-Hydroxyprogesterone; D000328:Adult; D001667:Binding, Competitive; D002996:Clomiphene / Q000494:pharmacology*; D003338:Corpus Luteum / Q000033:anatomy & histology / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D005260:Female; D006801:Humans; D006908:Hydroxyprogesterones / Q000378:metabolism; D008597:Menstrual Cycle / Q000187:drug effects / Q000378:metabolism*; D009929:Organ Size / Q000187:drug effects; D009994:Osmolar Concentration; D011374:Progesterone / Q000378:metabolism; D011974:Receptors, LH / Q000378:metabolism*
D006908:Hydroxyprogesterones; D011974:Receptors, LH; D002996:Clomiphene; D011374:Progesterone; D019326:17-alpha-Hydroxyprogesterone