Biovars of coagulase-positive staphylococci isolated from bovine mastitic milk.
Fifty strains of coagulase-positive staphylococci associated with bovine mastitis were biotyped. Of them, 20 were identified as biovar C (cattle & sheep), 17 as biovar B (poultry & swine), 2 as biovar D (hares) and 1 as biovar F (pigeon & fox). Of the remaining strains, 2 were closely related to human biovar A, 2 failed one property to be classified as biovar B, 3 shared properties of biovars B and D and 3 could not be identified. Bovine strains belonging to biovar C formed an heterogeneous group showing differences in crystal violet growth type and production of alpha haemolysin. Only strains associated with biovar A were Tween 80 positive. The highest incidence of lytic reactions amongst all biovars was with phages 42 E (III) and 102 (IV). Antibiotic resistance was most frequently found in biovar C (65%). The 2 strains in biovar D, one classed as biovar C and one as intermediate, were enterotoxigenic (C or D toxins).
Departamento de Higiene y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad de León.
M L
ML
García López
M C
MC
García Fernández
M R
MR
García Armesto
M
M
Prieto
A
A
Otero
M F
MF
Fernández Alvarez
D000818:Animals; D001434:Bacteriophage Typing; D002417:Cattle; D003030:Coagulase / Q000032:analysis; D004352:Drug Resistance, Microbial; D004768:Enterotoxins / Q000032:analysis; D005260:Female; D006460:Hemolysin Proteins / Q000032:analysis; D008414:Mastitis, Bovine / Q000382:microbiology*; D008892:Milk / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000382:microbiology*; D013210:Staphylococcus / Q000145:classification / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism
D003030:Coagulase; D004768:Enterotoxins; D006460:Hemolysin Proteins; C019368:enterotoxin D, Staphylococcal; C034959:enterotoxin C, staphylococcal