[Relation between H2 production and energy-dependent metabolism of H+ and K+ ions in E.coli].
Anaerobically grown E. coli escape H2 into the medium during the operation of H(+)-K(+)-pump exchanging 2H+ from a cell for one K+ of the medium. Anaerobic cells grown in the nitrate medium as well as the aerobically grown bacteria possessed neither 2H+/K+ exchange system, nor the ability for H2 production. Introduction of N,N'-dicyclohexylcarbodiimide into the medium, the removal of external K+ or the decrease of external osmotic pressure blocked both the functioning of H(+)-K(+)-pump and H2 production. The substitution of glucose by lactate reduced the activity of bacteria without change in pump operation and H2 production. It is assumed that formate-hydrogen lease and H(+)-K(+)-pump are working in collaboration.
Bagramian
K A
KA
Martirosov
S M
SM
D002352:Carrier Proteins / Q000378:metabolism*; D004563:Electrochemistry; D004734:Energy Metabolism; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D027961:Potassium-Hydrogen Antiporters
D002352:Carrier Proteins; D027961:Potassium-Hydrogen Antiporters; D006859:Hydrogen; D011188:Potassium
Sviaz' mezhdu proizvodstvom H2 i énergozavisimym obmenom ionov H+ i K+ u bakteriĭ E.coli.