[The transformation of legionellae by plasmid DNA].
For the first time the possibility of the genetic transformation of L. pneumophila and L. bozemanii strains with the use of purified DNA of plasmids pUC19, pUC4K, pSC101 and RSF1010-pBR322 was shown. The frequency of transformation varied from 5.2 x 10(-6) to 5.8 x 10(-7), depending on the strain used in the experiment and plasmid DNA. In some of the transformants obtained in this investigation plasmid DNA whose molecular weight was similar to that of the plasmid DNA used for transformation was detected. The relatively stable preservation of plasmids pSC101 and RSF1010 in Legionella strains and the loss of plasmids pUC19, pUC4K and pBR322 in 80% of transformants during storage were shown.
B I
BI
Marakusha
E Iu
EIu
Feoktistova
I S
IS
Tartakovskiĭ
S V
SV
Prozorovskiĭ
D001431:Bacteriological Techniques; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005821:Genetic Techniques; D007875:Legionella / Q000235:genetics* / Q000254:growth & development; D009154:Mutation; D010957:Plasmids / Q000235:genetics*; D012641:Selection, Genetic; D014169:Transformation, Bacterial / Q000235:genetics*
D004269:DNA, Bacterial
Transformatsiia legionell plazmidnoĭ DNK.