Renin is sorted to the regulated secretory pathway in transfected PC12 cells by a mechanism which does not require expression of the pro-peptide.
The rat pheochromocytoma cell line PC12 targets secretory proteins into two distinct pathways. When DNA encoding human prorenin was transfected into PC12 cells, the protein was sorted into the regulated secretory pathway and released with similar kinetics to noradrenaline upon carbachol stimulation. To determine whether information for targeting prorenin lies within the pro-peptide we have transfected PC12 cells with a construct lacking the pro-peptide coding sequence. The transformed line secretes an apparently fully active enzyme and responds to carbachol stimulation with a rapid release of renin activity. We conclude that the pro-peptide of renin is not essential for targeting the protein to the regulated pathway in PC12 cells.
Department of Biochemistry, University of Leicester, England.
M A
MA
Chidgey
T M
TM
Harrison
D000310:Adrenal Gland Neoplasms / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D004792:Enzyme Precursors / Q000378:metabolism* / Q000502:physiology; D005786:Gene Expression Regulation; D005809:Genes, Regulator; D009638:Norepinephrine / Q000378:metabolism; D010673:Pheochromocytoma / Q000235:genetics / Q000378:metabolism; D051381:Rats; D012083:Renin / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D012636:Secretory Rate / Q000235:genetics; D014162:Transfection / Q000235:genetics*; D014407:Tumor Cells, Cultured
D004792:Enzyme Precursors; D002217:Carbachol; D012083:Renin; D009638:Norepinephrine