A highly sensitive quantitative bioassay for human granulocyte-macrophage colony-stimulating factor.
Based on the granulocyte-macrophage colony-stimulating factor (GM-CSF) dependency of a newly established human myeloid cell line GM/SO, we developed a highly specific and sensitive bioassay for human GM-CSF. The presence of bioactive GM-CSF could be determined by measuring the formazan concentration produced from MTT by the cells that survived and proliferated in the presence of either natural or recombinant human GM-CSF. With this assay we were able to quantify the level of GM-CSF in two human sera as well as in conditioned media from human bladder cell carcinoma cell line 5637, a human fibroblast line, and phytohemagglutinin-stimulated peripheral blood mononuclear cells. The sensitivity of the assay allows measurement of concentrations of GM-CSF as low as 0.1 U/ml.
Universität Erlangen-Nürnberg, Medizinische Klinik, Federal Republic of Germany.
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Oez
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Bührer
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Atzpodien
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JR
Kalden
D001681:Biological Assay; D002455:Cell Division; D002470:Cell Survival; D003470:Culture Media; D016207:Cytokines / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000378:metabolism; D005562:Formazans / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000032:analysis* / Q000097:blood / Q000494:pharmacology; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000473:pathology; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D010835:Phytohemagglutinins / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000378:metabolism
D003470:Culture Media; D016207:Cytokines; D005562:Formazans; D010835:Phytohemagglutinins; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor