Biochemical and genetic analysis of an alpha-mannosidase mutant from Saccharomyces cerevisiae.
A yeast mutant lacking non-specific alpha-mannosidase activity was found as a background marker during our search for dap2 mutants (Suárez-Rendueles, P. and Wolf, D. H. (1987) J. Bacteriol. 169, 4041-4048). The mutant (DPS-15) is characterized in detail. The mutation called amd1 segregates 2:2 in meiotic tetrads, indicating a single chromosomal gene mutation which is recessive. Diploids heterozygous for amd1 show gene dosage. Thus, it appears that AMD1 might be the structural gene for alpha-mannosidase. Results obtained with this mutant show that alpha-mannosidase is not a vital component of the vegetative cell cycle. The differentiation process of sporulation is not disturbed in homozygous mutant diploids. Mannose turnover does not seem to be altered in mutant cells.
Cueva
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Departamento de Biología Funcional, Universidad de Oviedo, Spain.
Bordallo
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Suárez Rendueles
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D005800:Genes, Fungal; D008361:Mannosidases / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D043323:alpha-Mannosidase
D008361:Mannosidases; D043323:alpha-Mannosidase