Expression of the sulfonamide resistance gene from plasmid R46.
The expression of the sul I gene from plasmid R46, a wide host range plasmid of the IncN incompatibility group, was studied in Escherichia coli. Using a promoter test vector, a promoter was detected upstream of the sul I gene. From a nuclease protection experiment, the transcription was determined to start 360 bp upstream of the coding sequence. Two putative promoter -35 and -10 sequences were found upstream from the predicted transcription start. The presence of this promoter sequence in other R factors was discussed in relation with previous data showing that the sul I genes were transcribed from other promoters. The translation product of the sul I gene was detected in minicells. Its size indicates that the translation starts at the first ATG codon found in the open reading frame.
John Innes Institute, AFRC Institute of Plant Science Research, Norwich, United Kingdom.
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F
Guerineau
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L
Brooks
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P
Mullineaux
D001483:Base Sequence; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005091:Exons; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D011815:R Factors / Q000235:genetics*; D013449:Sulfonamides / Q000494:pharmacology*; D014158:Transcription, Genetic
D013449:Sulfonamides