Localization of a 24-kilodalton glycoprotein in adult Schistosoma mansoni using immunofluorescence and immunoelectron microscopy.
Studies on schistosome protective immune responses have focused mainly on antigens of the parasite's syncytial surface. One of the characterized schistosome antigens, a 24-kDa glycoprotein, has been considered important in mechanisms of immune evasion by the parasites. In the present study, using affinity-purified antibodies to the 24-kDa protein for immunofluorescence and immunoelectron microscopy, we demonstrated an association of the 24-kDa antigen with the discoid bodies (the major syncytial inclusion bodies; DBs) and the surface membrane complex (most likely the apical plasma membrane) of adult Schistosoma mansoni. This is consistent with previous observations that the 24-kDa antigen appeared to be localized to the syncytial membrane and DB fractions. The present results also support the suggestion that the DBs are the precursor organelles of the apical plasma membrane.
Department of Zoology, University of Western Ontario, London, Canada.
Y
Y
Zhou
S R
SR
Karcz
R B
RB
Podesta
M W
MW
Clarke
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology / Q000302:isolation & purification; D000947:Antigens, Helminth / Q000032:analysis*; D015153:Blotting, Western; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000032:analysis*; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D012550:Schistosoma mansoni / Q000032:analysis* / Q000276:immunology / Q000648:ultrastructure
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D006023:Glycoproteins