[Free amino acid and synthesis of polyamines in Plasmodium-berghei-infected RBC].
The contents of free amino acids and the activity of ornithine decarboxylase in infected RBC of chloroquine-sensitive strain of Plasmodium berghei(CS) were similar to those in chloroquine-resistant strain (CR). After treatment with chloroquine (10mg/kg im) no effect on the formation of free amino acids was found after 20th, but chloroquine (5mg/kg im) inhibited the enzymatic activity of the CS RBC by 79.6% whereas that of the CR by 55.7%. The contents of spermidine in CS and CR infected RBC were found to be 139 +/- 27 and 528 +/- 140 nmol/10(9) infected respectively. Cycloleucine inhibited the formation of spermidine by 44% in CS and 57% in CR groups, while co-administration of methionine (100mg/kg) with cycloleucine (80mg/kg) could reverse the inhibitory effect and raise the contents of spermidine to 294 +/- 56 and 657 +/- 129 nmol/10(9) infected RBC in CS and CR groups respectively. This indicates that methionine had significant effect on the formation of spermidine in CS strain, but there was still a remarkable difference, when compared with CR strain. It is suggested that the activities of S-adenosyl-methionine synthetase and other enzymes involved in polyamine formation might be the causal factors.
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine.
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Chang
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Huang
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Wang
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Wang
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D002738:Chloroquine / Q000494:pharmacology; D004351:Drug Resistance; D004912:Erythrocytes / Q000378:metabolism / Q000469:parasitology*; D066298:In Vitro Techniques; D008715:Methionine / Q000494:pharmacology; D051379:Mice; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D010962:Plasmodium berghei / Q000187:drug effects / Q000502:physiology*; D013095:Spermidine / Q000096:biosynthesis*
D000596:Amino Acids; D002738:Chloroquine; D008715:Methionine; D009955:Ornithine Decarboxylase; D013095:Spermidine