A short, highly repetitive element in intron -1 of the human c-Ha-ras gene acts as a block to transcriptional readthrough by a viral promoter.
We have identified a short, highly repetitive element within intron -1 of the human c-Ha-ras gene. This element was found to be transcribed in both orientations and to be homologous to heterogeneous nonpolyadenylated transcripts. The repetitive element blocked transcriptional readthrough from a strong upstream viral promoter but allowed unimpaired readthrough from the c-Has-ras promoter. We suggest that it may serve to prevent excessive transcription into the coding region of the gene under such circumstances as viral insertion.
Department of Genetics, College of Physicians and Surgeons of Columbia University, New York, New York 10032.
N F
NF
Lowndes
P
P
Bushel
L
L
Mendelsohn
J
J
Wu
M Y
MY
Yen
M
M
Allan
D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D005091:Exons; D011905:Genes, ras; D006801:Humans; D007438:Introns; D004915:Leukemia, Erythroblastic, Acute; D011401:Promoter Regions, Genetic; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012190:Retroviridae / Q000235:genetics*; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic; D014162:Transfection; D001749:Urinary Bladder Neoplasms
D012333:RNA, Messenger; D015500:Chloramphenicol O-Acetyltransferase