A short, highly repetitive element in intron -1 of the human c-Ha-ras gene acts as a block to transcriptional readthrough by a viral promoter.
We have identified a short, highly repetitive element within intron -1 of the human c-Ha-ras gene. This element was found to be transcribed in both orientations and to be homologous to heterogeneous nonpolyadenylated transcripts. The repetitive element blocked transcriptional readthrough from a strong upstream viral promoter but allowed unimpaired readthrough from the c-Has-ras promoter. We suggest that it may serve to prevent excessive transcription into the coding region of the gene under such circumstances as viral insertion.
Lowndes
N F
NF
Department of Genetics, College of Physicians and Surgeons of Columbia University, New York, New York 10032.
Bushel
P
P
Mendelsohn
L
L
Wu
J
J
Yen
M Y
MY
Allan
M
M
D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D005091:Exons; D011905:Genes, ras; D006801:Humans; D007438:Introns; D004915:Leukemia, Erythroblastic, Acute; D011401:Promoter Regions, Genetic; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012190:Retroviridae / Q000235:genetics*; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic; D014162:Transfection; D001749:Urinary Bladder Neoplasms
D012333:RNA, Messenger; D015500:Chloramphenicol O-Acetyltransferase