Separation of the Hageman factor fragment from human serum albumin by chromatography on blue sepharose CL-6B.
The separation of the Hageman factor fragment (HFf) activity from human serum albumin by chromatography on Blue Sepharose CL-6B is described. The complete separation cannot be achieved in a single chromatography step due to complex formation between HFf and albumin.
Institute of Hematology and Blood Transfusion, Prague, Czechoslovakia.
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Simonianová
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Hrkal
D002852:Chromatography, Ion Exchange; D005174:Factor XII / Q000302:isolation & purification*; D006801:Humans; D008024:Ligands; D010446:Peptide Fragments / Q000302:isolation & purification*; D012685:Sepharose / Q000031:analogs & derivatives; D012709:Serum Albumin / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D008024:Ligands; D010446:Peptide Fragments; D012709:Serum Albumin; C017842:cibacron blue-sepharose; D005174:Factor XII; D012685:Sepharose