A chimera antibody erythroimmunoassay for detecting HBsAg in human sera.
A highly efficient chimera antibody, a monoclonal anti-hepatitis-B-surface (anti-HBs) antibody coupled with polyclonal anti-sheep-red-blood-cell (anti-SRBC) antibody was prepared using a heterobifunctional reagent, N-succinimidyl-3-(2-pyridyldithiopropionate) (SPDP). Using SRBC as a marker, we established a sensitive solid-phase chimera antibody erythroimmunoassay (CAEIA) according to Guesdon's method. The sensitivity of this assay was 2-20 times higher than the reverse passive haemagglutination assay (RPHA) for detecting HBsAg in serially diluted sera from 10 hepatitis B patients. The weakest quantity of HBsAg detected by this assay was 4.5 ng/ml, while RPHA was unable to detect less than 75 ng/ml of HBsAg. The assay was as sensitive as the enzyme-linked immunosorbent assay (ELISA) and gave more accurate, reproducible and stable results than ELISA; specificity was also satisfactory.
Department of Immunology, Institute of Infectious Diseases, Beijing PRC.
Y
Y
Chen
S C
SC
Yang
Q H
QH
Luo
D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002678:Chimera; D003432:Cross-Linking Reagents; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000276:immunology; D006509:Hepatitis B / Q000175:diagnosis; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006801:Humans; D007124:Immunoenzyme Techniques; D013388:Succinimides
D000911:Antibodies, Monoclonal; D003432:Cross-Linking Reagents; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D013388:Succinimides; C018151:N-succinimidyl 3-(2-pyridyldithio)propionate