Micro-enzyme immunoassay of vasoactive intestinal polypeptide (VIP)-like immunoreactive substance in bovine milk.
A sensitive and specific enzyme immunoassay for vasoactive intestinal polypeptide (VIP)-like immunoreactivity was developed with the use of synthetic carboxy-terminal (C-terminal) fragment (residue 11-28) of porcine VIP conjugated with beta-D-galactosidase and a second antibody-coated immunoplate. Using 4-methylumbelliferyl beta-D-galactopyranoside as a fluorogenic substrate, the minimum amount of VIP-like immunoreactive substance (VIP-IS) detectable by this method was 0.1 fmol/well (2.5 pmol/l). The level of VIP-IS in bovine foremilk was above 100 pmol/l, which was more than eightfold higher than that in normal milk.
Department of Hospital Pharmacy, Medical College of Oita, Japan.
M
M
Takeyama
K
K
Wakayama
F
F
Takayama
K
K
Kondo
N
N
Fujii
H
H
Yajima
D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D007124:Immunoenzyme Techniques; D008892:Milk / Q000276:immunology*; D014660:Vasoactive Intestinal Peptide / Q000276:immunology*
D014660:Vasoactive Intestinal Peptide