Phospholipid biosynthesis in Candida albicans: regulation by the precursors inositol and choline.
Phospholipid metabolism in the pathogenic fungus Candida albicans was examined. The phospholipid biosynthetic pathways of C. albicans were elucidated and were shown to be similar to those of Saccharomyces cerevisiae. However, marked differences were seen between these two fungi in the regulation of the pathways in response to exogenously provided precursors inositol and choline. In S. cerevisiae, the biosynthesis of phosphatidylcholine via methylation of phosphatidylethanolamine appears to be regulated in response to inositol and choline; provision of choline alone does not repress the activity of this pathway (G. M. Carman and S. A. Henry, Annu. Rev. Biochem. 58:636-669, 1989). The same pathway in C. albicans responds to the exogenous provision of choline. Possible explanations for the observed differences in regulation are discussed.
GLAXO Institute for Molecular Biology S.A., Geneva, Switzerland.
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D002176:Candida albicans / Q000254:growth & development / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002794:Choline / Q000378:metabolism*; D006706:Homeostasis; D007294:Inositol / Q000378:metabolism*; D007700:Kinetics; D008780:Methyltransferases / Q000378:metabolism; D008954:Models, Biological; D010710:Phosphates / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis* / Q000302:isolation & purification; D010761:Phosphorus Radioisotopes; D011865:Radioisotope Dilution Technique; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013104:Spheroplasts / Q000378:metabolism
D002250:Carbon Radioisotopes; D010710:Phosphates; D010743:Phospholipids; D010761:Phosphorus Radioisotopes; D007294:Inositol; D008780:Methyltransferases; D002794:Choline