Isolation and nucleotide sequence determination of a gene encoding a heat-stable enterotoxin of Escherichia coli.
An enterotoxin-producing strain of E. coli has been isolated from an infant patient in Shanghai Children Hospital and the gene of its heat-stable enterotoxin has been cloned and sequenced. The pre-pro-STI was composed of 72 amino acid residues corresponding to the encoding of 216 base pairs. There was only one nucleotide difference in the pro-part between this STI gene and the STIb gene reported in the literature. A guanosine base in our STI gene was substituted for a cytosine base in STIb gene resulting in a replacement of proline by alanine. Hence, the STI genes from different human sources are highly conserved though mutagenesis still occurs.
Shanghai Institute of Biochemistry, Academia Sinica, China.
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Zhou
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Shen
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D000595:Amino Acid Sequence; D001483:Base Sequence; D004768:Enterotoxins / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data
D004768:Enterotoxins