Domains of human c-myc protein required for autosuppression and cooperation with ras oncogenes are overlapping.
Amino acids 106 to 143 and 354 to 433 of the human c-myc protein (439 amino acids) were shown to be required for the protein to suppress c-myc gene transcription and were found to exactly overlap with those necessary for c-myc to cooperate with ras oncogenes in the transformation of rat embryo fibroblasts. The essential carboxyl-terminal region harbors structural motifs (a basic region, a helix-loop-helix motif, and a "leucine zipper"), which, in other proteins, can mediate dimerization and sequence-specific DNA binding.
Growth Control and Development Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields, London, United Kingdom.
L J
LJ
Penn
M W
MW
Brooks
E M
EM
Laufer
T D
TD
Littlewood
J P
JP
Morgenstern
G I
GI
Evan
W M
WM
Lee
H
H
Land
D000818:Animals; D002460:Cell Line; D011905:Genes, ras; D006801:Humans; D009154:Mutation; D011487:Protein Conformation; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes; D051381:Rats; D013489:Suppression, Genetic; D014158:Transcription, Genetic
D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D011505:Protein-Tyrosine Kinases