[A simple method for the isolation of immunoglobulin (Y) from the eggs of immunized hens].
Hens were vaccinated with Salmonella typhi-murium and with serum-IgG from a goat. Antibodies (IgY) were isolated from the eggs by ammonium sulphate precipitation. The concentration of egg antibodies was equal or superior to the concentration of serum antibodies of the hen. The longlasting antibody plateau of 9 and 28 weeks, respectively, provides evidence of the high yields of specific IgY which can be collected from a single immunized hen.
GTZ-BGA-Service-Labor im Institut für Veterinärmedizin, Bundesgesundheitsamtes, Berlin.
J
J
Wallmann
C
C
Staak
E
E
Luge
D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification*; D002645:Chickens / Q000276:immunology*; D004530:Egg Yolk; D005260:Female; D007136:Immunoglobulins / Q000302:isolation & purification*; D012486:Salmonella typhimurium / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary
D000914:Antibodies, Viral; C016512:IgY; D007136:Immunoglobulins
Einfache Methode zur Isolierung von Immunglobulin (Y) aus Eiern immunisierter Hühner.