An initiation zone of chromosomal DNA replication located upstream of the c-myc gene in proliferating HeLa cells.
Studies on origins of DNA replication in mammalian cells have long been hampered by a lack of methods sensitive enough for the localization of such origins in chromosomal DNA. We have employed a new method for mapping origins, based on polymerase chain reaction amplification of nascent strand segments, to examine replication initiated in vivo near the c-myc gene in human cells. Nascent DNA, pulse-labeled in unsynchronized HeLa cells, was size fractionated and purified by immunoprecipitation with anti-bromodeoxyuridine antibodies. Lengths of the nascent strands that allow polymerase chain reaction amplification were determined by hybridization to probes homologous to amplified segments and used to calculate the position of the origin. We found that DNA replication through the c-myc gene initiates in a zone centered approximately 1.5 kilobases upstream of exon I. Replication proceeds bidirectionally from the origin, as indicated by comparison of hybridization patterns for three amplified segments. The initiation zone includes segments of the c-myc locus previously reported to drive autonomous replication of plasmids in human cells.
Vassilev
L
L
Brookdale Center for Molecular Biology, Mount Sinai School of Medicine, New York, New York 10029.
Johnson
E M
EM
D002455:Cell Division; D002874:Chromosome Mapping; D002877:Chromosomes, Human; D004261:DNA Replication; D004273:DNA, Neoplasm / Q000235:genetics; D006367:HeLa Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes
D004273:DNA, Neoplasm; D015345:Oligonucleotide Probes; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D011505:Protein-Tyrosine Kinases