Transfected human beta-polymerase promoter contains a ras-responsive element.
beta-Polymerase is a vertebrate cellular DNA polymerase involved in gap-filling synthesis during some types of genomic DNA repair. We report that a cloned human beta-polymerase promoter in a transient expression assay is activated by p21v-rasH expression in NIH 3T3 cells. A decanucleotide palindromic element, GTGACGTCAC, at positions -49 to -40 in the promoter is required for this ras-mediated stimulation.
Laboratory of Biochemistry, National Cancer Institute, Bethesda, Maryland 20892.
P S
PS
Kedar
D R
DR
Lowy
S G
SG
Widen
S H
SH
Wilson
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D004256:DNA Polymerase I / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D011905:Genes, ras; D006801:Humans; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D015689:Oncogene Protein p21(ras) / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014162:Transfection
D011993:Recombinant Fusion Proteins; D015500:Chloramphenicol O-Acetyltransferase; D004256:DNA Polymerase I; D015689:Oncogene Protein p21(ras)