Two subunits of the canine signal peptidase complex are homologous to yeast SEC11 protein.
Canine microsomal signal peptidase activity was previously isolated as a complex of five subunits (25, 22/23, 21, 18, and 12 kDa). Two of the signal peptidase complex (SPC) subunits (23/23 and 21 kDa) have been cloned and sequenced. One of these, the 21-kDa subunit, was observed to be a mammalian homolog of SEC11 protein (Sec11p) (Greenburg, G., Shelness, G. S., and Blobel, G. (1989) J. Biol. Chem. 264, 15762-15765) a gene product essential for signal peptide processing and cell growth in yeast (Böhni, P.C., Deshqies, R.J., and Schekman, R.W. (1988) J. Cell Biol. 106, 1035-1042). cDNA clones for the 18-kDa SPC subunit have now been characterized and found to encode a second SEC11p homolog. Both the 18- and 21-kDa canine SPC subunits are integral membrane proteins by virtue of their resistance to alkaline extraction. Upon detergent solubilization, both proteins are found in a complex with the 22/23 kDa SPC subunit, the only SPC subunit containing N-linked oligosaccharide. No steady-state pool of canine Sec11p-like monomers is detected in microsomal membranes. Alkaline extraction of microsomes prior to solubilization or solubilization at alkaline pH causes partial dissociation of the SPC. The Sec11p-like subunits displaced from the complex under these conditions demonstrate no signal peptide processing activity by themselves. The existence of homologous subunits is common to a number of known protein complexes and provides further evidence that the association between SPC proteins observed in vitro may be physiologically relevant to the mechanism of signal peptide processing and perhaps protein translocation.
Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, New York 10021.
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004285:Dogs; D010450:Endopeptidases / Q000235:genetics* / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000201:enzymology; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D015151:Immunoblotting; D007425:Intracellular Membranes / Q000201:enzymology; D008565:Membrane Proteins / Q000235:genetics; D008861:Microsomes / Q000201:enzymology; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases; D016133:Polymerase Chain Reaction; D021382:Protein Sorting Signals / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D012689:Sequence Homology, Nucleic Acid; D012697:Serine Endopeptidases
D005656:Fungal Proteins; D006023:Glycoproteins; D008565:Membrane Proteins; D021382:Protein Sorting Signals; D029701:Saccharomyces cerevisiae Proteins; D004247:DNA; D010450:Endopeptidases; D010447:Peptide Hydrolases; C061149:SEC11 protein, S cerevisiae; D012697:Serine Endopeptidases; C026515:type I signal peptidase