Direct estimation of serological H-Y antigen by flow cytometry.
This study was undertaken to evaluate the efficiency of flow cytometry in the detection of the serological H-Y antigen, and to survey expression of H-Y in the normal human population. Peripheral blood leukocytes (granulocytes) were reacted with monoclonal H-Y antibody, gw-16, and with FITC-conjugated goat anti-mouse IgG, and then were scored for fluorescence in the flow cytometer. Assigned H-Y phenotype was correlated accurately with sex phenotype in 33 out of 38 women and 62 out of 65 men. Data were evaluated by exploratory data analysis (EDA).
Kent
M
M
College of Agricultural and Life Sciences, University of Wisconsin, Madison 53706-1284.
Wachtel
S
S
Thaler
H T
HT
D000911:Antibodies, Monoclonal; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D005455:Fluorescent Antibody Technique; D006098:Granulocytes / Q000032:analysis*; D006182:H-Y Antigen / Q000032:analysis*; D006801:Humans; D008297:Male; D010641:Phenotype; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012732:Sex Determination Analysis
D000911:Antibodies, Monoclonal; D006182:H-Y Antigen