A new, highly sensitive enzymic assay for human tryptase and its use for identification of tryptase inhibitors.
A chromogenic two-stage assay for human tryptase, a specific marker of mast cell activation, was developed based on the tryptase-induced conversion of prothrombin to thrombin. This assay proved to be more sensitive and reliable than measurements of amidolytic activity of tryptase with small synthetic substrates such as Bz-Arg-Nan and was suitable to detect tryptase activity in human body fluids. In addition, the assay was useful for studies of natural and recombinant inhibitors of tryptase.
Department of Clinical Chemistry and Clinical Biochemistry, University of Munich, FRG.
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SC
Dietze
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EA
Auerswald
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H
Fritz
D001711:Biotransformation; D004796:Clinical Enzyme Tests / Q000379:methods; D005260:Female; D006801:Humans; D008407:Mast Cells / Q000201:enzymology; D010447:Peptide Hydrolases / Q000032:analysis*; D011480:Protease Inhibitors / Q000032:analysis*; D011516:Prothrombin / Q000378:metabolism
D011480:Protease Inhibitors; D011516:Prothrombin; D010447:Peptide Hydrolases; C023073:tosylarginine methyl ester hydrolase