Grass pollen allergens: detection on pollen grain surface using membrane print technique.
A microtechnique is described to visualize pollen allergens by making prints from the surface of mature grains on nitrocellulose membrane. The allergen Lol p I is detected by specific monoclonal antibodies or specific IgE from sera of grass-pollen-allergic patients, visualized by the immunoperoxidase method. This technique offers the potential to study the distribution of individual allergens in aerobiology samples by bright-field microscopy, and to determine the sensitivity spectra of allergic individuals to known and unknown pollen sources.
School of Botany, University of Melbourne, Parkville, Australia.
P M
PM
O'Neill
M B
MB
Singh
R B
RB
Knox
D000485:Allergens; D000911:Antibodies, Monoclonal / Q000276:immunology; D052179:Antigens, Plant; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E; D007158:Immunologic Techniques; D010940:Plant Proteins; D006109:Poaceae / Q000032:analysis*; D011058:Pollen / Q000032:analysis*
D000485:Allergens; D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; C042814:Lol p I protein, Lolium perenne; D010940:Plant Proteins; D007073:Immunoglobulin E