NMR studies of a bacterial cell culture medium (LB broth): cyclic nucleotides in yeast extracts.
The composition of LB broth (tryptone, yeast extract and NaCl) was investigated by 1H,31P-NMR spectroscopy, FPLC and gel electrophoresis. An unexpected finding was the high level of 2'3'-cyclic nucleotides, detected by characteristic 31P-NMR resonances in the region 20-21 ppm, originating from the yeast component. 31P-NMR resonances for cyclic nucleotides were observed during the autolysis of Saccharomyces cerevisiae cells, and in model reactions of RNase with RNA.
Rayner
M H
MH
Department of Chemistry, Birkbeck College, Gordon House, Laboratory, London, U.K.
Sadler
P J
PJ
Scawen
M D
MD
D001431:Bacteriological Techniques; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000032:analysis*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D009712:Nucleotides, Cyclic / Q000378:metabolism; D012331:RNA, Fungal / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism
D003470:Culture Media; D009712:Nucleotides, Cyclic; D012331:RNA, Fungal