A novel surface antigen expressed by mature and activated rabbit T-lymphocytes.
The monoclonal antibody (Mab) 93C6 was used to identify a surface molecule expressed by mature and activated rabbit T-lymphocytes. This antigen was virtually absent in cells of the thymus (5% positive cells). All 93C6-positive cells expressed the rabbit pan-T marker L11-135 and proliferated in response to concanavalin A (Con A). When lymphocytes were activated in the presence of Con A, the expression of the 93C6 molecule was quantitatively increased. Con A stimulation alone did not markedly enhance expression of this antigen by thymocytes, but stimulation with both Con A and interleukin-2 (IL-2) resulted in a population of thymocytes that expressed the antigen in a manner quantitatively similar to that of mature T-cells in other tissues.
Department of Experimental Pathology, Walter Reed Army Institute of Research, Washington, D.C. 20307.
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Ruiz
M J
MJ
Roy
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M
Gordon
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D003208:Concanavalin A; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D007376:Interleukin-2; D007413:Intestinal Mucosa / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011233:Precipitin Tests; D011817:Rabbits; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D007376:Interleukin-2; D003208:Concanavalin A