Selection of a hyperproducing strain of Aspergillus niger for biosynthesis of citric acid on unusual carbon substrates.
Several tens of mutants were obtained by UV irradiation of a spore suspension of Aspergillus niger. Producers yielding large amounts of citric acid were selected on a modified Czapek agar containing methyl red as pH indicator. After a submerged cultivation in flasks with baffles, a mutant characterized by yellow pigmentation on wort agar and by yields of citric acid up to 74.6% in the medium containing glucose was chosen from 130 isolates tested.
Institute of Chemical Technology, Prague.
J
J
Pelechová
L
L
Petrová
E
E
Ujcová
L
L
Martínková
D001234:Aspergillus niger / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D002951:Citrates / Q000378:metabolism*; D019343:Citric Acid; D003470:Culture Media / Q000378:metabolism; D008828:Microbiological Techniques; D012641:Selection, Genetic
D002951:Citrates; D003470:Culture Media; D019343:Citric Acid; D002244:Carbon