Eosinophil colony-stimulating factor induced by administration of interleukin-2 into the pleural cavity of patients with malignant pleurisy.
Systemic administration of interleukin (IL)-2 to patients with malignant diseases induces peripheral eosinophilia. In the present study, to clarify the mechanism of eosinophilia induced by IL-2, we examined the changes in the number of eosinophils and eosinophil colony-stimulating factor (Eo-CSF) activity in the pleural fluids of six patients with malignant pleurisy caused by lung cancer or malignant mesothelioma during and after intrapleural administration of IL-2. Results showed that intrapleural administration of IL-2 induced marked eosinophilia in the pleural fluid and moderate eosinophilia in the peripheral blood, and that during IL-2 administration, marked Eo-CSF activity appeared in the pleural fluid before increase in the number of eosinophils, but that this activity did not appear in the peripheral blood. This Eo-CSF activity was inhibited by a combination of anti-IL-5 antibody, anti-IL-3 antibody, and anti-granulocyte/macrophage colony-stimulating factor (anti-GM-CSF) antibody, but not by each antibody alone. Chemotactic activity for eosinophils was also detected in the pleural fluid during IL-2 treatment. These results suggest that eosinophilia in the pleural fluid induced by IL-2 injection into the pleural cavity of patients with malignant pleurisy is due to the Eo-CSF activities of various components, including IL-5, IL-3, and GM-CSF, and chemotactic factors for eosinophils induced locally in the pleural cavity by IL-2.
Third Department of Internal Medicine, University of Tokushima, School of Medicine, Japan.
Y
Y
Nakamura
T
T
Ozaki
H
H
Yanagawa
S
S
Yasuoka
T
T
Ogura
D001853:Bone Marrow / Q000473:pathology; D002277:Carcinoma / Q000473:pathology / Q000628:therapy*; D002634:Chemotaxis, Leukocyte; D003115:Colony-Stimulating Factors / Q000502:physiology*; D004802:Eosinophilia / Q000209:etiology*; D004804:Eosinophils / Q000648:ultrastructure; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000502:physiology; D006801:Humans; D007376:Interleukin-2 / Q000627:therapeutic use*; D007377:Interleukin-3 / Q000502:physiology; D015848:Interleukin-5 / Q000502:physiology; D007958:Leukocyte Count; D008654:Mesothelioma / Q000473:pathology / Q000628:therapy*; D016066:Pleural Effusion, Malignant / Q000473:pathology / Q000628:therapy*
D003115:Colony-Stimulating Factors; D007376:Interleukin-2; D007377:Interleukin-3; D015848:Interleukin-5; D016178:Granulocyte-Macrophage Colony-Stimulating Factor