The mas oncogene as a neural peptide receptor: expression, regulation and mechanism of action.
The human mas oncogene, which renders transfected NIH/3T3 cells tumorigenic, was identified as a subtype of angiotensin receptor by transient expression in Xenopus oocytes and stable expression in the mammalian neuronal cell line, NG115-401L. The mas receptor preferentially recognizes angiotensin III, and is expressed at high levels in brain. The mas/angiotensin receptor functions through the breakdown of inositol lipids and can drive DNA synthesis, unlike another inositol-linked peptide receptor, that for bradykinin. Comparative analysis of several early biochemical events elicited by either angiotensin or bradykinin stimulation of mas-transfected cells has not indicated a specific difference correlated with mitogenic activity. In particular, the inositol lipid kinase, phosphatidylinositol-3-kinase, thought to be involved in the mitogenic mechanism of platelet-derived growth factor receptors, is unaffected by activation of mas. These results have shown that a proto-oncogene encodes a neural peptide receptor, indicating that peptide receptors may be involved in differentiation and proliferation processes, as are other identified proto-oncogenes.
MRC Molecular Neurobiology Unit, MRC Centre, Cambridge, UK.
M R
MR
Hanley
W T
WT
Cheung
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Hawkins
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Poyner
H P
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Benton
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Blair
T R
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Jackson
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Goedert
D000595:Amino Acid Sequence; D000805:Angiotensin III / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D009857:Oncogenes; D011487:Protein Conformation; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes; D011945:Receptors, Angiotensin / Q000235:genetics*; D014162:Transfection
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011945:Receptors, Angiotensin; D000805:Angiotensin III