In vitro insulin-like growth factor I interaction with cartilage cells derived from postnatal animals.
This paper reports data on the in vitro effects of insulin-like growth factor I (IGF-I) and basic fibroblast growth factor (bFGF) on the phenotypic expression of epiphyseal chondrocytes grown in serum-free (SF) culture medium. bFGF mostly stimulates chondrocyte DNA and inhibits sulfated proteoglycan synthesis and type II collagen mRNA. On the contrary, IGF-I is poorly mitogenic but strongly stimulates protein synthesis and type II collagen mRNA. In addition, IGF-I prevents the expression of type I collagen gene. Lastly, chondrocytes cultured in SF medium are able to locally produce IGF-I peptides. In conclusion, IGF-I and bFGF have opposite effects on the phenotypic expression of chondrocytes in vitro: bFGF is mostly mitogenic and IGF-I appears to be a differentiating factor.
Inserm U 30, Hôpital des Enfants-Malades, Paris, France.
D
D
Demarquay
M F
MF
Dumontier
L
L
Tsagris
J
J
Bourguignon
V
V
Nataf
M T
MT
Corvol
D000818:Animals; D003094:Collagen / Q000235:genetics; D004247:DNA / Q000096:biosynthesis; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D015870:Gene Expression; D006132:Growth Plate / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis / Q000494:pharmacology*; D010641:Phenotype; D011509:Proteoglycans / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D013431:Sulfates / Q000378:metabolism
D011509:Proteoglycans; D012333:RNA, Messenger; D013431:Sulfates; D016222:Fibroblast Growth Factor 2; D007334:Insulin-Like Growth Factor I; D003094:Collagen; D004247:DNA