Determination of plasmid-associated hydrophobicity of Yersinia enterocolitica by the salting-out test.
The hydrophobicity of the cell surface of Yersinia enterocolitica was investigated by the salting-out test. It was shown that plasmid-containing strains grown at 37 degrees C aggregated at a final concentration of (NH4)2SO4 of between 0.3 to 0.5 M. In contrast, the plasmid-free derivatives did not show aggregation even at a concentration of 2.0 M(NH4)2SO4. Therefore, the salting-out test can be utilized as a simple and reliable method for distinguishing plasmid-containing, virulent strains of Yersinia enterocolitica from their plasmid-free derivatives. This was also confirmed by the use of 30 clinical isolates of virulent Y. enterocolitica.
Institute Ruder Bosković, Zagreb, Yugoslavia.
N
N
Lers
I
I
Stojiljković
Z
Z
Trgovcević
S
S
Kalenić
E
E
Salaj-Smic
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D006801:Humans; D010957:Plasmids; D013499:Surface Properties; D014774:Virulence; D015008:Yersinia enterocolitica / Q000235:genetics / Q000472:pathogenicity*
D001425:Bacterial Outer Membrane Proteins