Selection of medium for serum-free primary culture of adult rat hepatocytes.
To select a suitable medium for serum-free primary culture of adult rat hepatocytes, ten commercially-available synthetic media were compared for their ability to maintain the cells under serum-free and serum-supplemented conditions with special reference to attachment, survival and albumin secretion. It was found that Williams' medium E and DM-160 medium were the best among the ten media for maintaining hepatocytes under serum-free conditions in primary culture.
Division of Pathology, Okayama University Medical School, Japan.
M
M
Miyazaki
L Y
LY
Bai
J
J
Sato
D000418:Albumins / Q000378:metabolism; D000818:Animals; D001769:Blood; D002448:Cell Adhesion; D002470:Cell Survival; D003470:Culture Media; D003584:Cytological Techniques; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D051381:Rats
D000418:Albumins; D003470:Culture Media