Sensitivity of freshly isolated ovine adipocytes to inhibition of lipolysis by insulin.
1. Ovine adipocytes were isolated in the presence of adenosine to minimize cell damage and were incubated at a low cell concentration. 2. Insulin sensitivity of lipid metabolism was retained. 3. Insulin inhibited basal lipolysis by 61% and isoproterenol- and adenosine deaminase-stimulated lipolysis by 84%. 4. Insulin increased glucose conversion to cell lipid by 3-fold.
Department of Biochemistry and Genetics, University of Newcastle upon Tyne, Medical School, UK.
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Cochrane
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Rogers
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008066:Lipolysis / Q000187:drug effects*; D012756:Sheep
D007328:Insulin; D005947:Glucose; D005990:Glycerol