Removal of HIV antigens and HIV-infected cells in vitro using immunomagnetic beads.
Human anti-HIV antibody-coated magnetic beads and magnetic particle concentrators were used to eliminate HIV antigens and the infected cells in vitro. Fifty micrograms/ml of the antibody coated on 30 mg/ml of tosyl-activated beads, and 10 min of reaction time between the antigens and the immunobeads were sufficient to eliminate the virus antigens from phosphate buffered saline, serum and peripheral blood. HIV-infected cells were eliminated in vitro, but not completely. This method will be useful in eliminating viral antigens and infected cells from clinical material such as blood and blood products.
Division of AIDS Virus, National Institute of Health, Tokyo, Japan.
H
H
Ushijima
H
H
Honma
H
H
Tsuchie
T
T
Kitamura
I
I
Takahashi
D002469:Cell Separation / Q000379:methods*; D006678:HIV / Q000302:isolation & purification* / Q000648:ultrastructure; D015488:HIV Antigens / Q000097:blood / Q000302:isolation & purification*; D006801:Humans; D007124:Immunoenzyme Techniques; D008280:Magnetics; D008863:Microspheres
D015488:HIV Antigens