[Detection of protease-sensitive factors with properties of beta-type transforming growth factor in early loach embryos].
The blastoderm and yolk of Misgurnus fossilis L. late blastulae were subjected to acid-ethanol extraction and gel filtration on biogel P10 in 1 M CH3COOH. This resulted in isolation of polypeptides that induce substrate-independent proliferation of NIH-3T3 mouse fibroblasts in presence of epidermal growth factor. They did not compete for epidermal growth factor receptors of A-431 cells and inhibited substrate-independent proliferation of human lung carcinoma A-549 cells. These properties of the growth factor-like polypeptides from loach embryos are similar to those of transforming growth factor beta.
Ia Ia
IaIa
Fedishin
R S
RS
Stoĭka
S I
SI
Kusen'
D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D001756:Blastoderm / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects; D003531:Cypriniformes / Q000196:embryology* / Q000502:physiology; D004347:Drug Interactions; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D051379:Mice; D010447:Peptide Hydrolases / Q000494:pharmacology*; D015291:Transforming Growth Factors / Q000302:isolation & purification / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology; D015017:Yolk Sac / Q000502:physiology
D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors; D010447:Peptide Hydrolases
Obnaruzhenie proteazochuvstvitel'nykh faktorov so svoĭstvami transformiruiushchego faktora rosta beta-tipa v rannikh zarodyshakh v'iuna.