Phagocytosis induced by yeast cells in canine granulocytes: a methodological study.
A phagocytic function assay of canine granulocytes was established. This method allows the proportion of active granulocytes to be estimated as well as the number of adhered and ingested yeast cells. The influence of different factors on phagocytosis was studied. Temperature variation within the interval 36-41 degrees C did not affect phagocytosis. The incubation time for optimal phagocytosis of yeast cells was 35 min. The opsonization procedure giving the optimal phagocytosis was purified IgG and serum together.
Department of Medicine and Surgery, Veterinary Faculty, Swedish University of Agricultural Sciences, Uppsala, Sweden.
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Trowald-Wigh
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Thorén-Tolling
D000818:Animals; D004285:Dogs / Q000276:immunology*; D005260:Female; D006098:Granulocytes / Q000276:immunology*; D007700:Kinetics; D008297:Male; D009895:Opsonin Proteins; D010587:Phagocytosis; D012441:Saccharomyces cerevisiae / Q000276:immunology*; D013696:Temperature
D009895:Opsonin Proteins