Improved methods for genotype determination of human alcohol dehydrogenase (ADH) at ADH 2 and ADH 3 loci by using polymerase chain reaction-directed mutagenesis.
The human gene for producing alcohol dehydrogenase (ADH; EC 1.1.1.1) is polymorphic at ADH 2 and ADH 3 loci. Until now, the study of this polymorphism required liver biopsy or allele-specific radioactive probes. We have used directed mutagenesis by the polymerase chain reaction (PCR) to amplify and analyze the genotype of ADH 2 and ADH 3 loci. Thus, we could determine easily and unambiguously the complete genotype at these two loci by using a microsample of blood and restriction fragment length polymorphism after DNA amplification by PCR.
Groppi
A
A
Laboratoire de Génétique, CNRS UA 542, Talence, France.
Begueret
J
J
Iron
A
A
D000426:Alcohol Dehydrogenase / Q000097:blood / Q000235:genetics*; D000483:Alleles; D001483:Base Sequence; D001665:Binding Sites; D005838:Genotype; D006801:Humans; D007527:Isoenzymes / Q000097:blood / Q000235:genetics*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D016133:Polymerase Chain Reaction
D007527:Isoenzymes; D000426:Alcohol Dehydrogenase