DNA methylation in Trypanosoma cruzi.
DNA isolated from the protozoan Trypanosoma cruzi has been found to contain 5-methylcytosine. Analysis of T. cruzi DNA by both HpaII and MspI restriction endonucleases suggests that the sequence -CCGG- is not methylated. Probably T. cruzi DNA also contains N6-methyladenine. This report constitutes the first clear demonstration of the presence of methylated bases in the nuclear DNA from trypanosomes.
Rojas
M V
MV
Department of Cell Biology and Genetics, School of Medicine, University of Chile, Santiago.
Galanti
N
N
D000225:Adenine / Q000031:analogs & derivatives / Q000032:analysis; D000818:Animals; D004247:DNA / Q000096:biosynthesis / Q000302:isolation & purification; D006868:Hydrolysis; D008715:Methionine / Q000378:metabolism; D008745:Methylation; D008970:Molecular Weight; D011685:Purine Nucleotides / Q000302:isolation & purification; D011742:Pyrimidine Nucleotides / Q000302:isolation & purification; D014349:Trypanosoma cruzi / Q000032:analysis* / Q000378:metabolism
D000225:Adenine; D004247:DNA; D008715:Methionine; D011685:Purine Nucleotides; D011742:Pyrimidine Nucleotides; C000730567:6-methyladenine