Comparison of different membranes for use in the colony-immunoblot technique.
We compared five different supports (Whatman paper filters Nos. 1, 5, and 40, nitrocellulose, and Nylon 66) for their suitability in the colony-immunoblot (CIB) technique. Results indicate that Whatman No. 5 filter paper recovered 94-98% of the bacterial colonies tested, were more resistant to tearing than the other Whatman papers tested, and showed reduced cross-reactions as compared with nitrocellulose membranes. Whatman No. 5 filters are 20 times less expensive than the nitrocellulose membranes usually used in the CIB technique. We thus adopted the former for our ecological studies of the murine oral cavity.
Département de biochimie, Université Laval, Québec, Canada.
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Rodrigue
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Lavoie
D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D003101:Collodion; D005374:Filtration / Q000295:instrumentation; D015151:Immunoblotting / Q000379:methods*; D051379:Mice; D009055:Mouth / Q000382:microbiology
D003101:Collodion