Use of HL cells for improved isolation and passage of Chlamydia pneumoniae.
We compared growth of the recently discovered respiratory pathogen Chlamydia pneumoniae in McCoy, HeLa 229, BHK-21, and HL cells. When cells were not pretreated with DEAE-dextran, HL cells had significantly higher mean numbers of inclusion-forming units (IFUs) on initial inoculation than the other cell lines. When cells were pretreated with DEAE-dextran, HeLa 229 and HL cells had equivalent mean numbers of IFUs on initial inoculation. HL cells had strikingly higher mean numbers of IFUs in passage than HeLa 229, BHK-21, or McCoy cells. In addition, HL cells did not require pretreatment with DEAE-dextran and could be used from 2 to 4 days after seeding. We conclude that HL cells are an excellent cell culture system for laboratory propagation of C. pneumoniae and may be a more sensitive cell line for initial isolation.
Cles
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Department of Medicine, University of Washington, Seattle 98195.
Stamm
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D000818:Animals; D001431:Bacteriological Techniques; D002460:Cell Line; D002689:Chlamydia / Q000254:growth & development / Q000302:isolation & purification*; D003637:DEAE-Dextran; D005069:Evaluation Studies as Topic; D006801:Humans
D003637:DEAE-Dextran