[Comparison of three methods for detecting Campylobacter pylori and measurement of human antibody titers against the whole organisms].
In order to detect Campylobacter pylori in the gastric mucosa, three different methods as 1) culture of the organisms, 2) immunostaining by monoclonal antibody against the cells, and 3) urease test were compared. In the disease group, positive % of each methods was 64, 75 and 74. However, positive % common to the three methods was only 48. The corresponding rate of culture and urease tests, and that of culture and staining methods was 81% and 60%, respectively. Therefore, it was concluded that urease test was a useful method, and that the three methods were necessary to confirm the existence of the organisms in the gastric mucosa. Total positive % of culture and staining methods in disease and control groups was 77 and 31, respectively, indicating that positive % of the disease group was much higher than that of the control group. Anti C. pylori titer was compared between culture-positive and culture-negative patients by ELISA. The titer of the former was significantly higher than that of the latter.
Yokota
K
K
Department of Microbiology, Sapporo Medical College.
Oguma
K
K
Yoshida
H
H
Takayama
Y
Y
Sugiyama
T
T
Yabana
T
T
Yachi
A
A
Kurebayashi
Y
Y
Isogai
H
H
Isogai
E
E
D000907:Antibodies, Bacterial / Q000032:analysis*; D001431:Bacteriological Techniques; D002167:Campylobacter / Q000276:immunology / Q000302:isolation & purification*; D004381:Duodenal Ulcer / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D005753:Gastric Mucosa / Q000382:microbiology*; D005756:Gastritis / Q000382:microbiology; D006801:Humans; D013276:Stomach Ulcer / Q000382:microbiology
D000907:Antibodies, Bacterial