Kinetics of protease hydrolysis of extended peptide substrates: measurement by flow-injection analysis.
A flow-injection analysis (FIA) system was developed to study the enzyme-catalyzed hydrolysis of synthetic peptides, each of which contained one scissile bond. The concentrations of alpha-amino groups in reactions mixtures were determined by FIA with o-phthalaldehyde as a fluorescence reagent. The method allows a rapid, precise, and sensitive determination of kinetic constants for proteases acting on extended peptide substrates.
Chong
G L
GL
Department of Biochemistry, Kansas State University, Manhattan 66506.
Davis
L C
LC
Reeck
G R
GR
D002851:Chromatography, High Pressure Liquid; D005470:Fluorometry; D006868:Hydrolysis; D007700:Kinetics; D010447:Peptide Hydrolases / Q000378:metabolism*; D010455:Peptides / Q000138:chemical synthesis / Q000378:metabolism*; D013379:Substrate Specificity; D009764:o-Phthalaldehyde
D010455:Peptides; D009764:o-Phthalaldehyde; D010447:Peptide Hydrolases