Infection of human fibroblasts and osteoblast-like cells with HIV-1.
Primary human skin- and lung-derived fibroblast cell cultures and continuous human osteoblast-like and fibroblast-like cell lines were infected with different strains of HIV-1. Infection was measured at the single-cell level using the immunoperoxidase staining method to detect viral proteins. No cytopathic effects were observed in HIV-1-infected cell cultures. One continuous cell line (LC5), derived from embryonic lung, was readily infectable with HIV-1 and showed continuous production of infectious virus. Infection of LC5 cells could be blocked with anti-CD4 monoclonal antibodies. These findings indicate that fibroblasts of skin and lung, and osteogenic cells may be considered as potential target cells for HIV-1, thereby possibly contributing to the establishment of local HIV reservoirs.
Mellert
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Abteilung für Molekulare Zellpathologie, Gesellschaft für Strahlen- und Umweltforschung München (GSF), Neuherberg, FRG.
Kleinschmidt
A
A
Schmidt
J
J
Festl
H
H
Emler
S
S
Roth
W K
WK
Erfle
V
V
D000163:Acquired Immunodeficiency Syndrome / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D015704:CD4 Antigens / Q000276:immunology; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000276:immunology; D005347:Fibroblasts / Q000382:microbiology*; D015497:HIV-1 / Q000472:pathogenicity*; D006801:Humans; D007124:Immunoenzyme Techniques; D010006:Osteoblasts / Q000382:microbiology*; D012333:RNA, Messenger / Q000276:immunology
D015704:CD4 Antigens; D004279:DNA, Viral; D012333:RNA, Messenger