[Frequency and distribution of beta-lactamases in 1792 strains of Klebsiella pneumoniae in France between 1985 and 1988].
In october 1985, 1987 and 1988, all the clinical isolates of K. pneumoniae (respectively 530, 654, 590 strains) were collected in 20 hospitals. The beta-lactamases were identified by analytical isoelectrofocusing and by substrate and inhibition profiles. 76 to 81% of the strains produced only one beta-lactamase: SHV-1 type, pI 7.7 (61 to 65%) or PI 7.1 (14%). The TEM-1 betalactamase (pI 5.4) was produced in 1985 by 21% of the strains, 9% in 1987, and 11% in 1988: TEM-2, pI 5.6 by 2% in 1985-87-88. The extended broad spectrum beta-lactamases, able to hydrolyse amino-thiazol-oximino-beta-lactam antibiotics, TEM or SHV type enzymes (SHV-2, pI 7.7, SHV-3, pI 7.1; SHV-4/CAZ-5, pI 7.8; SHV-5/CAZ-4 pI 8.2; CTX-1/TEM-3, pI 6.3) were also detected: 0.75% of the strain (3 strains) in 1985, 8.4% (55 strains) in 1987, 11% (65 strains) in 1988. These extended broad spectrum beta-lactamases were found in 2 hospitals in 1985, 10 in 1987 and 9 in 1988.
Laboratoire de Biologie Médicale, Hôpital Militaire Bégin, St-Mandé.
A
A
Thabaut
J
J
Acar
P
P
Allouch
G
G
Arlet
L
L
Berardi-Grassias
E
E
Bergogne-Bérézin
Y
Y
Brun
Y
Y
Buisson
G
G
Chabanon
R
R
Cluzel
D005602:France; D006801:Humans; D066298:In Vitro Techniques; D007525:Isoelectric Focusing; D007710:Klebsiella Infections / Q000201:enzymology / Q000453:epidemiology*; D007711:Klebsiella pneumoniae / Q000145:classification / Q000201:enzymology*; D015337:Multicenter Studies as Topic; D010957:Plasmids; D001618:beta-Lactamases / Q000032:analysis*
D001618:beta-Lactamases
Fréquence et distribution des bêta-lactamases chez 1,792 souches de Klebsiella pneumoniae isolées en France entre 1985 et 1988.