The use of flow cytometry to monitor chitin synthesis in regenerating protoplasts of Candida albicans.
Flow cytometry was used to monitor chitin synthesis in regenerating protoplasts of the yeast Candida albicans. Comparisons of cells stained with Calcofluor White, a fluorochrome with known affinity for chitin, and cells incubated in the presence of N-[3H]-acetylglucosamine, the precursor substrate for chitin, showed a linear relationship between fluorescence and incorporation of label over time. Changes in both the fluorescence and light scatter of regenerating protoplasts treated with inhibitors of fungal chitin synthase were also quantitated by flow cytometry.
Hector
R F
RF
Cutter Biological, Berkeley, CA.
Braun
P C
PC
Hart
J T
JT
Kamarck
M E
ME
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology; D001557:Benzenesulfonates; D002176:Candida albicans / Q000187:drug effects / Q000502:physiology*; D002686:Chitin / Q000096:biosynthesis*; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D011523:Protoplasts / Q000187:drug effects / Q000502:physiology*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D001557:Benzenesulfonates; D005456:Fluorescent Dyes; D002686:Chitin; C007061:C.I. Fluorescent Brightening Agent 28; C011952:nikkomycin