The effect of radiation therapy combined with natural killer cells against spontaneous murine fibrosarcoma.
The effect of radiation therapy combined with lymphoid cells against spontaneous murine fibrosarcoma (FSa-II) was investigated both in vivo and in vitro. In the in vivo experiment, syngeneic C3H mice were divided into 3 groups. Animals in the first group were injected with 1 x 10(5) tumor cells into the right hind leg. Animals in the second and third groups were injected with 1 x 10(5) tumor cells mixed with 1 x 10(7) normal lymphoid cells (NLC) or effector lymphoid cells (ELC), respectively. ELC were obtained from spleen and lymph nodes of FSa-II-bearing mice and incubated in vitro for 40 hr to eliminate suppressor T cell function. NLC were obtained from normal mice and incubated in the same way. Irradiation was given using 137Cs unit 3 days after cell inoculation. 12 out of 14 mice (85.7%) inoculated with tumor cells mixed with NLC did not show any tumor growth at 60 Gy local irradiation. 12 out of 21 mice (57.1%) inoculated with tumor cells alone and 6 out of 10 (60%) with tumor cells mixed with ELC rejected tumors at the same radiation dose. This synergistic effect with NLC was not observed when NLC was inoculated after irradiation, indicating that lymphoid cells should be in contact with tumor cells before irradiation. In the 51Cr release assay, lymphoid cells obtained from whole body irradiated (WBI) mice showed 17.8% lysis without irradiation and 28.8% lysis at 5 Gy irradiation. Untreated NLC showed almost no cytotoxic effect at the same radiation dose. This synergistic effect disappeared when WBI lymphoid cells were treated with anti asialo GM1 and complement.(ABSTRACT TRUNCATED AT 250 WORDS)
Department of Neurological Surgery, University of Tsukuba, Ibaraki, Japan.
K
K
Nakagawa
F
F
Yoshida
N
N
Omori
T
T
Tsunoda
T
T
Nose
D000818:Animals; D002470:Cell Survival / Q000528:radiation effects; D003131:Combined Modality Therapy; D005260:Female; D005354:Fibrosarcoma / Q000532:radiotherapy / Q000628:therapy*; D007167:Immunotherapy; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000276:immunology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000528:radiation effects / Q000637:transplantation; D014916:Whole-Body Irradiation