[Study of the conditions for reproducing the processes of antibiotic biosynthesis. Determining the limiting stage of the process of mass transfer of oxygen].
A theoretical method for estimating limiting stage of oxygen mass transfer is proposed. The method is based on calculation and comparison of particular resistance of separate stages of mass transfer: mass transfer through the gas-liquid boundary, oxygen transport in the liquid phase, mass transfer through the liquid-cell boundary and oxygen consumption by microbial cells. With the proposed method, particular resistance was calculated and conditions for oxygen mass transfer were analyzed in the biosynthesis of sisomicin as an example. As a result the conditions for reproducibility of the process in apparatus of various size were determined.
L M
LM
Kuz'mina
V V
VV
Biriukov
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001419:Bacteria / Q000378:metabolism*; D001431:Bacteriological Techniques / Q000295:instrumentation*; D001692:Biological Transport; D003470:Culture Media; D066298:In Vitro Techniques; D007218:Industrial Microbiology / Q000295:instrumentation / Q000379:methods*; D008433:Mathematics; D008954:Models, Biological; D010100:Oxygen / Q000493:pharmacokinetics*; D010101:Oxygen Consumption
D000900:Anti-Bacterial Agents; D003470:Culture Media; D010100:Oxygen
Issledovanie usloviĭ vosproizvodimosti protsessov biosinteza antibiotikov. Opredelenie limitiruiushcheĭ stadii protsessa massoperedachi kisloroda.