Production of a novel monoclonal antibody to porcine adipocyte plasma membrane.
The adipocyte plasma membrane is composed of specific intrinsic, integral, and extrinsic proteins. The study of adipocyte development, morphology, and metabolism has been limited by a lack of characterization of these proteins. It seems likely that the adipocyte plasma membrane possesses adipocyte-specific proteins which may be linked to the unique identity of adipose tissues. To study the composition of the adipocyte plasma membrane, we produced a panel of monoclonal antibodies to purified adipocyte plasma membranes. Nineteen anti-adipocyte hybridoma cell lines were identified using fluorescent enzyme-linked immunosorbent assay, immunoblotting, and indirect immunofluorescence. A monoclonal antibody (designated LA-1) with reactivity toward a porcine adipocyte plasma membrane component was used for further adipocyte characterization. LA-1 reacted with a species-specific 64-kDa protein expressed in adipocyte plasma membranes but not in hepatocyte, erythrocyte, skeletal muscle, heart, spleen, kidney, stomach, small intestine, or large intestine plasma membranes. The LA-1 antibody provides a specific probe for this adipocyte surface protein marker.
Killefer
J
J
Department of Animal Science, Oregon State University, Corvallis 97331.
Hu
C Y
CY
D000273:Adipose Tissue / Q000166:cytology / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000941:Antigens / Q000276:immunology; D015153:Blotting, Western; D002462:Cell Membrane / Q000276:immunology; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D013045:Species Specificity; D013552:Swine
D000911:Antibodies, Monoclonal; D000941:Antigens