[Influenza viruses studied by fluorescent immunoassay with temporary resolution].
Monoclonal antibodies, epidemic strains of influenza A and B viruses, conjugates were studied by fluorescence immunoassay with temporary resolution using equipment of different companies. Differences and specificity of monoclonal antibodies to influenza A and B viruses were demonstrated. The highest sensitivity of the method with the test system used was determined, being 20 ng/ml of viral protein. The method was shown to be useful for influenza virus detection both in solutions containing purified virions and in virus-containing allantoic preparations. It was established that virological studies could be carried out using conjugates and knock-down microplates of the national make alongside with foreign ones.
Ivanova
V T
VT
Ponomareva
I V
IV
Shakhanina
K L
KL
Iakhno
M A
MA
Slepushkin
A N
AN
Savitskiĭ
A P
AP
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000032:analysis; D000918:Antibody Specificity / Q000276:immunology; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique / Q000295:instrumentation; D009980:Influenza A virus / Q000276:immunology*; D009981:Influenza B virus / Q000276:immunology*; D014758:Viral Core Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014758:Viral Core Proteins
Issledovanie virusov grippa metodom fliuorestsentnogo immunoanaliza s vremennym razresheniem.