Chicken GnRH II occurs together with mammalian GnRH in a South American species of marsupial (Monodelphis domestica).
Two molecular forms of gonadotropin-releasing hormone (GnRH) were demonstrated in hypothalamic extracts of M. domestica using high performance liquid chromatography and radioimmunoassay with specific GnRH antisera. One form eluted in the same position as synthetic mammalian GnRH and was quantified equally by two mammalian GnRH antisera, while the second form coeluted with synthetic chicken GnRH II and was quantified equally with two chicken GnRH II antisera. The finding of chicken GnRH II in a South American species of marsupial, which has previously been reported in some Australian species of marsupial and in species of Aves, Reptilia, Amphibia, Osteichthyes and Chondrichthyes, supports our hypothesis that this widespread structural variant may represent an early evolved and conserved form of GnRH.
Department of Chemical Pathology, University of Cape Town Medical School, South Africa.
J A
JA
King
L A
LA
Hinds
A E
AE
Mehl
N R
NR
Saunders
R P
RP
Millar
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000032:analysis; D007031:Hypothalamus / Q000032:analysis; D008322:Mammals; D008394:Marsupialia / Q000378:metabolism*; D008969:Molecular Sequence Data; D010906:Pituitary Hormone-Releasing Hormones / Q000032:analysis*; D011863:Radioimmunoassay; D013020:South America
D010906:Pituitary Hormone-Releasing Hormones; D007987:Gonadotropin-Releasing Hormone; C047717:LHRH, His(5)-Trp(7)-Tyr(8)-