Characterization of S9 fraction used in the third UKEMS collaborative trial.
The S9 preparation utilized by the collaborators in the third UKEMS collaborative study was assayed for the activities of three groups of enzymes and the effect of storage upon the activities. The results obtained indicate that storage of S9 at -196 degrees C for periods of up to 8 months did not unduly affect the activity of the cytochrome P450 isoenzymes measured.
Health and Safety Executive, Bootle, Merseyside, UK.
S A
SA
Hubbard
P J
PJ
Davis
D015660:7-Alkoxycoumarin O-Dealkylase / Q000378:metabolism; D000818:Animals; D001579:Benzopyrene Hydroxylase / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005037:Ethylmorphine-N-Demethylase / Q000378:metabolism; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000201:enzymology*; D015337:Multicenter Studies as Topic; D009152:Mutagenicity Tests; D010088:Oxidoreductases / Q000378:metabolism; D010090:Oxidoreductases, O-Demethylating / Q000378:metabolism; D051381:Rats; D013048:Specimen Handling
D003577:Cytochrome P-450 Enzyme System; C047335:7-methoxycoumarin-O-demethylase; D010088:Oxidoreductases; D010090:Oxidoreductases, O-Demethylating; D015660:7-Alkoxycoumarin O-Dealkylase; D001579:Benzopyrene Hydroxylase; D019363:Cytochrome P-450 CYP1A1; D005037:Ethylmorphine-N-Demethylase