Hormone regulated enzyme activities of plasma membrane of decidual endometrium of the rat.
Plasma membranes were purified from deciduoma of pseudopregnant rats and rat liver. Preparations contained 80% plasma membrane-derived material as based on electron microscope morphometry and analysis of enzyme markers. Several plasma membrane enzymes were tested for direct response to hormones. NADH-ferricyanide reductase of plasma membranes from both tissues was stimulated by glucagon and inhibited by insulin but was unresponsive to steroids. For steroids, responsiveness was limited to a reduction in NaF-stimulated adenylate cyclase activity by the steroid R5020. Thus, interaction of steroid hormones with plasma membranes, unlike that of glucagon and insulin, is not reflected in an altered activity of plasma membrane-bound dehydrogenases but may be exerted directly on adenylate cyclase.
Department of Biology, Suny College, Fredonia 14063.
W N
WN
Yunghans
N J
NJ
Karin
K
K
Ogborne
T
T
Desmond
D J
DJ
Morré
R N
RN
Day
S C
SC
Schiavi
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D003656:Decidua / Q000201:enzymology* / Q000648:ultrastructure; D004717:Endometrium / Q000201:enzymology* / Q000648:ultrastructure; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D005934:Glucagon / Q000494:pharmacology; D006728:Hormones / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000201:enzymology; D008854:Microscopy, Electron; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D010766:Phosphorylation; D011374:Progesterone / Q000494:pharmacology; D011397:Promegestone / Q000494:pharmacology; D011555:Pseudopregnancy; D051381:Rats
D004967:Estrogens; D006728:Hormones; D007328:Insulin; D011374:Progesterone; D005934:Glucagon; D011397:Promegestone; D009247:NADH, NADPH Oxidoreductases; C014227:ferricyanide reductase; D000262:Adenylyl Cyclases