Corticosterone regulates the expression of ADP-ribosylation factor messenger RNA and protein in rat cerebral cortex.
ADP-ribosylation factors (ARFs) comprise a family of small GTP-binding proteins found in brain and other tissues. Recent studies have demonstrated that the expression of the larger heterotrimeric guanine nucleotide binding proteins is under control by steroid hormones. Therefore, in the present study, we examined the influence of glucocorticoids on the expression of ARF mRNA and protein. using specific cDNA probes and antisera, respectively. Chronic administration of corticosterone (7 days) significantly increased levels of mRNA for ARF1 and ARF3, two subtypes of ARF, in rat cerebral cortex. Chronic administration of corticosterone was also found to increase levels of ARF immunoreactivity in this brain region. However, 1-day administration of corticosterone did not influence levels of mRNA for either ARF1 or ARF3. In contrast to corticosterone, bilateral adrenalectomy (7 days after surgery) was found to decrease ARF1 and ARF3 message relative to sham controls; this effect of adrenalectomy was reversed by corticosterone treatment. These results demonstrate that the expression of ARF is under hormonal control and may underlie aspects of glucocorticoid action on neuronal function.
Duman
R S
RS
Department of Psychiatry, Yale University School of Medicine, New Haven, Connecticut.
Winston
S M
SM
Clark
J A
JA
Nestler
E J
EJ
D020823:ADP-Ribosylation Factor 1; D020727:ADP-Ribosylation Factors; D000246:Adenosine Diphosphate Ribose / Q000235:genetics* / Q000378:metabolism; D000315:Adrenalectomy; D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D003345:Corticosterone / Q000502:physiology*; D019204:GTP-Binding Proteins / Q000235:genetics*; D015151:Immunoblotting; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D012333:RNA, Messenger; D000246:Adenosine Diphosphate Ribose; D019204:GTP-Binding Proteins; D020823:ADP-Ribosylation Factor 1; D020727:ADP-Ribosylation Factors; D003345:Corticosterone