DNA methylation in Trypanosoma cruzi.
DNA isolated from the protozoan Trypanosoma cruzi has been found to contain 5-methylcytosine. Analysis of T. cruzi DNA by both HpaII and MspI restriction endonucleases suggests that the sequence -CCGG- is not methylated. Probably T. cruzi DNA also contains N6-methyladenine. This report constitutes the first clear demonstration of the presence of methylated bases in the nuclear DNA from trypanosomes.
Department of Cell Biology and Genetics, School of Medicine, University of Chile, Santiago.
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Rojas
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Galanti
D000225:Adenine / Q000031:analogs & derivatives / Q000032:analysis; D000818:Animals; D004247:DNA / Q000096:biosynthesis / Q000302:isolation & purification; D006868:Hydrolysis; D008715:Methionine / Q000378:metabolism; D008745:Methylation; D008970:Molecular Weight; D011685:Purine Nucleotides / Q000302:isolation & purification; D011742:Pyrimidine Nucleotides / Q000302:isolation & purification; D014349:Trypanosoma cruzi / Q000032:analysis* / Q000378:metabolism
D000225:Adenine; D004247:DNA; D008715:Methionine; D011685:Purine Nucleotides; D011742:Pyrimidine Nucleotides; C000730567:6-methyladenine