[Ultrastructure of metacercarial membrane and immunohistochemical localization of metacercarial membrane reaction of Paragonimus westermani].
The ultrastructure of the tegument of the early excysted P. westermani metacercariae was observed with transmission electron microscope. The tegument was fine and dense in texture, containing a lot of disk-like bodies and a few mitochondria. The outer-plasma membrane was clear and intact. After the metacercariae were incubated in rabbit anti-serum for 3 hours, metacercarial membrane reaction (MMR) appeared closely to the outer plasma membrane and the tegument was damaged. Indirect peroxidase-labelled immunohistochemical technique was used to identify the location of the MMR at ultrastructural level. The result showed that the antibody-antigen complex was localized on the tegumental surface of the worm. The data constituted the evidence that the MMR resulted from the antibody-antigen reaction and the tegumental surface was the reaction site for the MMR.
Liao
M M
MM
Shanghai Medical University.
Huan
P F
PF
Wen
T H
TH
D000818:Animals; D007150:Immunohistochemistry; D007814:Larva; D010238:Paragonimus / Q000648:ultrastructure*; D011817:Rabbits