Complete amino acid sequence of bovine glia maturation factor beta.
The protein glia maturation factor beta, isolated from bovine brain, has been sequenced by automated Edman degradation and tandem mass spectrometry of overlapped peptide fragments generated by cyanogen bromide cleavage and enzymatic digestion with trypsin, chymotrypsin, and endoproteinases Asp-N and Lys-C. The protein has 141 amino acid residues and possesses no potential N-glycosylation sites. It contains three cysteines (at positions 7, 86, and 95), three methionines (at positions 33, 101, and 102), and one tryptophan (at position 132). The blocked amino terminus as determined by tandem mass spectrometry is an N-acetylated serine. The carboxyl terminus is a histidine. To our knowledge, the sequence shows no significant homology with other sequenced proteins. The molecular weight calculated from the sequence information is 16,582.
Department of Neurology (Division of Neurochemistry and Neurobiology), University of Iowa College of Medicine, Iowa City.
R
R
Lim
A
A
Zaheer
W S
WS
Lane
D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D003488:Cyanogen Bromide; D020904:Glia Maturation Factor; D006133:Growth Substances / Q000235:genetics*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000302:isolation & purification; D010446:Peptide Fragments / Q000302:isolation & purification; D010447:Peptide Hydrolases
D020904:Glia Maturation Factor; D006133:Growth Substances; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D010447:Peptide Hydrolases; D003488:Cyanogen Bromide