Enzyme immunoassay for methionine-enkephalin sulfoxide.
1. An immunoassay technique employing an enzyme-labelled ligand was developed for the sulfoxide derivative of the opioid peptide methionine-enkephalin (MOE). 2. The sensitivity of the enzyme immunoassay (EIA) was approximately twofold higher than that achieved in the radio-immunoassay (RIA) using tritiated ligand. Cross-reactivities of leucine-enkephalin and beta-endorphin with the EIA were less than 0.1%, while that with Gly-Gly-Phe-Met and oxidized Gly-Gly-Phe-Met were 2.5% and 10.2%, respectively. 3. These results indicate that the EIA is a useful alternative to the RIA for the assay of small peptides.
Tiong
G K
GK
Department of Pharmacology, Monash University, Clayton, Victoria, Australia.
Olley
J E
JE
D003429:Cross Reactions; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000276:immunology; D007124:Immunoenzyme Techniques; D011863:Radioimmunoassay
D004744:Enkephalin, Methionine; C029596:enkephalin-Met, sulfoxide-