Autoradiography using storage phosphor technology.
We describe the application of photostimulable storage phosphor imaging plates to autoradiography of samples labeled with 32P, 14C and 35S. Imaging plates can detect a 32P-labeled sample at an exposure level of 1 disintegration/mm2, and 14C and 35S at 25 disintegrations/mm2. Compared to X-ray film, imaging plates offer increased sensitivity of 15- to 250-fold for 32P and 20- to 100-fold for 14C and 35S. Resolution is in the 0.3 mm range and is therefore suitable for any gel or blot application.
Molecular Dynamics, Sunnyvale, CA 94086.
R F
RF
Johnston
S C
SC
Pickett
D L
DL
Barker
D001345:Autoradiography / Q000379:methods*; D002250:Carbon Radioisotopes; D010759:Phosphorus Isotopes; D012680:Sensitivity and Specificity; D013460:Sulfur Isotopes; D014962:X-Ray Film
D002250:Carbon Radioisotopes; D010759:Phosphorus Isotopes; D013460:Sulfur Isotopes