The presence of an antigen reactive with a human autoantibody in Trichosia pubescens (Diptera: Sciaridae) and its association with certain transcriptionally active regions of the genome.
The antigens in HeLa and Trichosia pubescens cells, recognized by sera from patients with rheumatic diseases containing anti-Ku antibodies, were compared by means of immunoprecipitation of labeled cell extracts. The autoantibodies present in the tested sera precipitate at least two polypeptides of approximately Mr = 70,000 and Mr = 80,000 in HeLa cell extracts and a polypeptide of approximately Mr = 72,000 in Trichosia salivary gland cell extracts. The distribution of the insect antigen in chromatin was studied in salivary gland polytene chromosomes by indirect immunofluorescent staining with sera from two different patients. Both sera react with certain transcriptionally active chromosomal sites. The presence of the antigen in polytene chromosomes is strictly dependent on transcription, as no reaction is observed in the same sites before or after gene activation. Other sites, such as the nucleolar organizing region, are very active in transcription but never reacted with the anti-Ku positive sera. These results show that the insect antigen is associated with transcription-related processes of a subset of the chromosomal loci of T. pubescens. The anti-Ku positive sera react with a highly conserved antigen, which may serve a very important and similar role in the cellular metabolism of both insect and mammalian cells.
Department of Biochemistry, Tufts University School of Medicine, Boston, MA 02111.
J M
JM
Amabis
D C
DC
Amabis
J
J
Kaburaki
B D
BD
Stollar
D000818:Animals; D034961:Antigens, Nuclear; D000954:Antigens, Surface / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D001323:Autoantibodies / Q000276:immunology*; D002875:Chromosomes / Q000276:immunology; D004265:DNA Helicases; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D004175:Diptera / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D000072200:Ku Autoantigen; D011233:Precipitin Tests; D014158:Transcription, Genetic
D034961:Antigens, Nuclear; D000954:Antigens, Surface; D001323:Autoantibodies; D004268:DNA-Binding Proteins; D004265:DNA Helicases; C106893:XRCC5 protein, human; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen