Establishment of new human prostatic cancer cell line (JCA-1).
The establishment of a new human prostatic cancer cell line is described. This cell line was derived from a poorly to moderately differentiated prostatic adenocarcinoma. It has been maintained in tissue culture for fourteen months and has been passed fifty-two times. This cell line has an ability to form colonies in soft agar suspension cultures, and also is transplantable to nude mice. Tumors grown in nude mice revealed a poorly differentiated adenocarcinoma with positive PSA staining. Acid phosphatase activity was detected in freeze-thawed cells by enzymatic assay. A karyotype analysis demonstrated aneuploidy with a model chromosomal number of 69 and six marker chromosomes.
Muraki
J
J
Department of Urology, New York Medical College, Valhalla.
Addonizio
J C
JC
Choudhury
M S
MS
Fischer
J
J
Eshghi
M
M
Davidian
M M
MM
Shapiro
L R
LR
Wilmot
P L
PL
Nagamatsu
G R
GR
Chiao
J W
JW
D000135:Acid Phosphatase / Q000378:metabolism; D000230:Adenocarcinoma / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D000368:Aged; D000782:Aneuploidy; D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007621:Karyotyping; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011471:Prostatic Neoplasms / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor; D000135:Acid Phosphatase