[Histogenesis of the clear cytoplasm in the tumor cells of smooth muscle origin].
Perinuclear clear cytoplasm observed in the light microscopic specimens of the tumor cells of smooth muscle origin is in general, understood as the artefact caused by the formalin fixation. However, the precise mechanisms of the histogenesis of clear cytoplasm are still not clear. We observed the clear cytoplasm directly by mean of the electron microscopy of materials detached from light microscopic specimen. Furthermore, we observed the light microscopic specimens made by varying types of methods, examining whether the clear cytoplasm was present or not. The electron microscopy of materials detached from light microscopic specimens revealed the band-like defects of cytoplasm along the long axes of tumor cells. These defects were thought to result from the falling off of cytoplasm. The 1 mu section of the epon embedded block derived from the paraffin embedded block for light microscopic specimen presented no clear cytoplasm, suggesting that the cytoplasm falls off at the procedure of deparaffinization and staining. Although the specimen of conventional frozen section showed no clear cytoplasm, the specimen made by the frozen sectioning after formalin fixation revealed clear cytoplasm. Consequently, it is thought that the fixation of the tissue before the sectioning makes the cytoplasm fragile, thereafter, the cytoplasm falls off at the procedure of deparaffinization and staining.
Pathology Division, Nagasaki University Hospital.
T
T
Hayashi
O
O
Shimada
M
M
Anami
M
M
Karasuyama
N
N
Sato
N
N
Muraoka
N
N
Tsuda
D000328:Adult; D000368:Aged; D003593:Cytoplasm / Q000473:pathology / Q000648:ultrastructure; D005260:Female; D006652:Histological Techniques; D006801:Humans; D007889:Leiomyoma / Q000473:pathology* / Q000648:ultrastructure; D007890:Leiomyosarcoma / Q000473:pathology* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D009130:Muscle, Smooth / Q000473:pathology* / Q000648:ultrastructure