The cauliflower mosaic virus open reading frame VII product can be expressed in Saccharomyces cerevisiae but is not detected in infected plants.
Antiserum was prepared against a synthetic peptide corresponding to the N-terminal 20 amino acids of the protein encoded by cauliflower mosaic virus (CaMV) open reading frame VII (ORF VII). This antiserum was used to detect the expression of CaMV ORF VII either in Saccharomyces cerevisiae transformed by an expression vector containing CaMV ORF VII or in CaMV-infected plants. Only in S. cerevisiae has a 14-kilodalton protein been detected.
Institut de Biologie Moléculaire des Plantes du C.N.R.S., Strasbourg, France.
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Wurch
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Kirchherr
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Mesnard
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Lebeurier
D001937:Brassica / Q000235:genetics / Q000382:microbiology*; D002874:Chromosome Mapping; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005814:Genes, Viral; D015151:Immunoblotting; D009029:Mosaic Viruses / Q000235:genetics*; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics / Q000302:isolation & purification
D014764:Viral Proteins