[Evaluation of a modified immunomagnetic procedure for the purging of neuroblastoma cells from bone marrow].
The immunomagnetic depletion method for removing tumor cells from bone marrow, previously refined using a Burkitt lymphoma model, was tested with neuroblastoma cells. The efficiency of depletion was quantified by immunofluorescence with a detection limit of 3.3 log of cell depletion corresponding to the elimination of 99.84% of an initial tumor cell content of 10%. A panel of five monoclonal antibodies (UJ13A; UJ127.11; UJ181.4; alpha-Thy1; H11) purged 2.8 log of SKNBE and LAN-1 cells, while two of these antibodies as single agents allowed only for a 1.7 log (UJ13A) and a 1.7 to 2.0 log (alpha-Thy1) depletion. This underlines the advantage of an antibody panel for neuroblastoma purging. The new antibody S-L 11.14, an IgG2a against small cell lung cancer which recognizes 90% of neuroblastoma cells purged 2.8 log.
Abteilung für Hämatologie und Onkologie, Universität Essen, GHS.
B
B
Kremens
V
V
Combaret
M C
MC
Favrot
J C
JC
Laurent
T
T
Philip
D000911:Antibodies, Monoclonal / Q000276:immunology; D001854:Bone Marrow Cells; D002469:Cell Separation / Q000379:methods*; D006801:Humans; D007158:Immunologic Techniques; D008280:Magnetics; D009447:Neuroblastoma / Q000473:pathology*
D000911:Antibodies, Monoclonal
Evaluation eines modifizierten immunomagnetischen Verfahrens zur Entfernung von Neuroblastomzellen aus Knochenmark.