Amplification of HIV-1 provirus from cerebrospinal fluid and its correlation with neurologic disease.
The polymerase chain reaction (PCR) was used to detect human immunodeficiency virus type 1 (HIV-1) proviral sequences (gag and env) in nucleated cells from the cerebrospinal fluid (CSF) of 31 HIV-1-positive patients, and the results were compared with clinical and radiologic evidence of neurologic disease. Provirus was detected in 21 patients, of whom 20 had neurologic abnormalities. Provirus was not detected in another 6, all of whom were neurologically normal. No neurologic disease has developed in 4 of these 6 patients for whom 12.8 months of follow-up is available. PCR of CSF nucleated cells from HIV-positive patients provides early, rapid, direct evidence of neurologic involvement.
Department of Medicine, Duke University Medical Center, Durham, North Carolina.
S
S
Shaunak
R E
RE
Albright
M E
ME
Klotman
S C
SC
Henry
J A
JA
Bartlett
J D
JD
Hamilton
D015526:AIDS Dementia Complex / Q000382:microbiology; D000328:Adult; D001483:Base Sequence; D002555:Cerebrospinal Fluid / Q000382:microbiology*; D002648:Child; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005500:Follow-Up Studies; D015752:Genes, env; D015750:Genes, gag; D015658:HIV Infections / Q000150:complications / Q000382:microbiology*; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D008969:Molecular Sequence Data; D009422:Nervous System Diseases / Q000150:complications / Q000382:microbiology*; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics* / Q000302:isolation & purification; D012189:Retrospective Studies; D014057:Tomography, X-Ray Computed
D004279:DNA, Viral; D015345:Oligonucleotide Probes