The rate of bulk flow from the Golgi to the plasma membrane.
A truncated analog of the backbone of sphingomyelin and glycolipids was synthesized. This truncated C8C8 ceramide was soluble in water (but was still able to cross cell membranes) and was utilized by the Golgi apparatus of living cells to produce water-soluble truncated phospholipids and glycolipids that were then secreted into the medium. Sphingomyelin is synthesized in a proximal (likely the cis) Golgi compartment. At 37 degrees C in CHO cells, the sphingomyelin analog is secreted with a half time of about 10 min. With this rate of bulk flow, no special signal is needed to pass through the Golgi to the plasma membrane. At 30 degrees C the half time of secretion of a lumenal ER marker is about 18 min, and that of the truncated sphingomyelin is about 14 min. Comparison of these rates sets an upper limit of about 4 min for half of the ER to be drained into the proximal Golgi at 30 degrees C.
Institut für Biochemie I, Universität Heidelberg, Federal Republic of Germany.
A
A
Karrenbauer
D
D
Jeckel
W
W
Just
R
R
Birk
R R
RR
Schmidt
J E
JE
Rothman
F T
FT
Wieland
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D006224:Cricetinae; D004058:Diffusion; D005963:Glucosylceramides / Q000378:metabolism; D006026:Glycoside Hydrolases; D006056:Golgi Apparatus / Q000378:metabolism*; D007700:Kinetics; D013058:Mass Spectrometry; D008954:Models, Biological; D013107:Sphingolipids / Q000378:metabolism*; D013108:Sphingomyelin Phosphodiesterase; D013110:Sphingosine / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000378:metabolism; D014316:Tritium
C066099:2,3-N-octanoylsphingosine; D005963:Glucosylceramides; D013107:Sphingolipids; D014316:Tritium; D013108:Sphingomyelin Phosphodiesterase; D006026:Glycoside Hydrolases; D013110:Sphingosine