[A new site-specific endodeoxyribonuclease EcoHI].
A new sitespecific endonuclease of the II class EcoHI has been isolated from Escherichia coli strain and characterized. Restriction endonuclease EcoHI recognises the nucleotide sequence C C (C/G) G G with the cleavage site between the fourth and fifth nucleotide. It is an isoshizomer of the restriction endonuclease CauII. The yield of enzyme is 2500 units of activity per 1 g of biomass. The producing strain Escherichia coli HI is nonpathogenic, easily grown with the antibiotic resistance markers permitting to cultivate the strain under selective conditions.
Glatman
L I
LI
Terekhov
A A
AA
Kalnin
K V
KV
Bolotin
A P
AP
Rebentish
B A
BA
D001483:Base Sequence; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics
C035801:CCSGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C037794:YGGCCR-specific type II deoxyribonucleases
Novaia saĭtspetsificheskaia éndodezoksiribonukleaza EcoHI.