Bioassay of interleukin-1 in serum and plasma following removal of inhibitory activity with polyethylene glycol.
Serum and plasma normally inhibit the responsiveness of the indicator cells used in bioassays for interleukin-1 (IL-1). A precipitation step with 12% polyethylene glycol (PEG) was found to remove material responsible for such inhibition. The IL-1 bioassay could be performed in the presence of a 3% concentration of PEG and recovery of added natural IL-1 from plasma or serum was essentially complete.
Hopkins
S J
SJ
University of Manchester Rheumatic Diseases Centre, Hope Hospital, Salford, U.K.
Humphreys
M
M
D000818:Animals; D001681:Biological Assay / Q000379:methods; D001798:Blood Proteins / Q000187:drug effects; D006801:Humans; D007375:Interleukin-1 / Q000037:antagonists & inhibitors / Q000097:blood*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D051381:Rats; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured
D001798:Blood Proteins; D007375:Interleukin-1; D011994:Recombinant Proteins; D011092:Polyethylene Glycols