Determination of plasma cell labelling index with bromodeoxyuridine using a double immunoenzymatic technique.
Myeloma plasma cells were double stained using peroxidase and alkaline phosphatase labelled monoclonal anti-BrdU and anti-intracytoplasmic immunoglobulins. Samples were methanol fixed; DNA was denatured with formamide. The results allowed easy identification of plasma cells, their cytological examination and the calculation of percentage of plasma cells in S phase. Good correlation was found with the labelling index obtained with tritiated thymidine.
Analytic Cytology Laboratory, C. Bernard University, E. Herriot Hospital, Lyon, France.
M
M
Ffrench
J P
JP
Magaud
P
P
Duhaut
P A
PA
Bryon
J J
JJ
Viala
D001973:Bromodeoxyuridine; D002453:Cell Cycle; D006801:Humans; D007124:Immunoenzyme Techniques; D010265:Paraproteinemias / Q000097:blood* / Q000473:pathology; D010950:Plasma Cells / Q000473:pathology*
D001973:Bromodeoxyuridine