Association of rns homologs with colonization factor antigens in clinical Escherichia coli isolates.
rns is a trans-acting positive regulatory factor required for expression of the colonization factor antigen II (CFA/II) antigens CS1 and CS2 (J. Caron, L. M. Coffield, and J. R. Scott, Proc. Natl. Acad. Sci. USA 86:963-967, 1989). All 35 CFA/II-positive strains hybridized with a rns gene probe, as did all 10 CFA/I strains and all 4 CS4 strains. Hybridization with rns was detected in 25% of non-enterotoxigenic Escherichia coli strains and was not detected in enteric pathogens with low G + C content.
Department of Microbiology and Immunology, Emory University Health Sciences Center, Atlanta, Georgia 30322.
J
J
Caron
D R
DR
Maneval
J B
JB
Kaper
J R
JR
Scott
D000942:Antigens, Bacterial / Q000235:genetics*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004926:Escherichia coli / Q000145:classification / Q000235:genetics*; D036781:Fimbriae Proteins; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D015183:Restriction Mapping
D000942:Antigens, Bacterial; D015342:DNA Probes; D004269:DNA, Bacterial; C022924:colonization factor antigens; D036781:Fimbriae Proteins