Effect of brodimoprim on Mycobacterium leprae in vitro and in mouse foot-pads.
The new in vitro screening system reported earlier was adopted to determine anti-M. leprae activity of a dihydrofolate reductase inhibitor, brodimoprim, and the results were compared with those obtained using mouse foot-pad technique. Even though the MIC of brodimoprim against M. leprae was very high compared to other commonly used anti-leprosy drugs, in combination with dapsone it showed a remarkable synergistic activity in inhibiting the growth of M. leprae at concentrations much lower than the MICs of each of the drugs used singly. Similar effects were also demonstrated in mouse foot-pad experiments.
Dhople
A M
AM
Department of Biological Sciences, Florida Institute of Technology, Melbourne 32901.
Ortega
I
I
Seydel
J K
JK
Gardner
G D
GD
D000818:Animals; D003622:Dapsone / Q000494:pharmacology; D004357:Drug Synergism; D007917:Leprostatic Agents / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009166:Mycobacterium leprae / Q000187:drug effects*; D014295:Trimethoprim / Q000031:analogs & derivatives* / Q000494:pharmacology
D007917:Leprostatic Agents; D003622:Dapsone; D014295:Trimethoprim; C037079:brodimoprim