Direct serum injection high-performance liquid chromatographic method for the simultaneous determination of phenobarbital, carbamazepine and phenytoin.
A method is described for the simultaneous measurement of serum levels of three antiepileptic drugs, phenobarbital, phenytoin and carbamazepine, by direct injection high-performance liquid chromatography on a 25-cm Pinkerton internal surface reversed-phase (ISRP) column. Several commonly available compounds were tested and found not to co-chromatograph with the three drugs of interest or the internal standard, 5-(p-methylphenyl)-5-phenylhydantoin. Results obtained on patients' samples with this method compared well with those from enzyme-multiplied immunoassay technique (EMIT).
Department of Chemical Pathology, Norfolk Hospital, Norwich, U.K.
S J
SJ
Rainbow
C M
CM
Dawson
T R
TR
Tickner
D002220:Carbamazepine / Q000097:blood* / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004827:Epilepsy / Q000097:blood / Q000188:drug therapy; D006801:Humans; D007124:Immunoenzyme Techniques; D010634:Phenobarbital / Q000097:blood* / Q000627:therapeutic use; D010672:Phenytoin / Q000097:blood* / Q000627:therapeutic use
D002220:Carbamazepine; D010672:Phenytoin; D010634:Phenobarbital