In vivo effects of cyclosporin A on murine B-cells responding to type III pneumococcal polysaccharide.
The effect of cyclosporin A (CSA) on the antibody response to pneumococcal polysaccharide type III (a T-independent class 2 antigen) was investigated in mice. A single oral CSA administration (50 mg/kg) was able to depress (40%) the primary antibody response evaluated as spleen plaque-forming cells. Repeated treatments (12-50 mg/kg x 5) resulted in a higher degree of inhibition (80%) of anti-SIII response. Both single and repeated CSA treatments were active only when administered concomitantly with or after immunization, whereas no effects were seen with drug pretreatment. Comparable inhibitions of anti-SIII response were observed in control and nude mice suggesting a direct effect of CSA on B-cells.
Sozzani
S
S
Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
Sironi
M
M
Magistrelli
M
M
Luini
W
W
Spreafico
F
F
Vecchi
A
A
D000284:Administration, Oral; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D003524:Cyclosporins / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D006386:Hemagglutination Tests; D006462:Hemolytic Plaque Technique; D007114:Immunization; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011135:Polysaccharides, Bacterial / Q000008:administration & dosage / Q000276:immunology*; D013154:Spleen / Q000378:metabolism
D003524:Cyclosporins; D011135:Polysaccharides, Bacterial; C026604:pneumococcal polysaccharide, type III