Direct binding and lectin-mediated binding of erythrocytes to haemocytes of the insect, Extatosoma tiaratum.
Treatment of Extatosoma haemocytes on a monolayer with fixed rabbit erythrocytes (RE) in the presence of Ca2+ results in ca. 11% plasmatocytes (PL) and 58% spreading granular (SG) cells rosetting. Pretreatment of haemocytes with lactose, D-galactose, or asialofetuin reduces rosetting, suggesting that the membrane-associated receptors involved may be lectins. The percentage of rosetting PL and SG cells is increased by a number of heterologous lectins (indicating the presence of D-galactose, mannose, and Glu Nac receptors on these haemocytes) and a D-galactose specific lectin isolated from the serum of the insect. The latter acts as a bridging molecule and requires Ca2+ to bind to specific receptors on both haemocytes and fixed RE.
School of Biological Sciences, University College of Swansea, Wales, U.K.
E H
EH
Richards
N A
NA
Ratcliffe
D000371:Agglutination; D000818:Animals; D001667:Binding, Competitive; D002118:Calcium / Q000502:physiology; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D005404:Fixatives; D005690:Galactose; D005696:Galactosidases; D006434:Hemocytes / Q000276:immunology* / Q000378:metabolism; D007785:Lactose; D037102:Lectins / Q000502:physiology*; D008361:Mannosidases; D009987:Orthoptera / Q000276:immunology*; D011817:Rabbits; D012397:Rosette Formation; D043323:alpha-Mannosidase
D005404:Fixatives; D037102:Lectins; D005696:Galactosidases; D008361:Mannosidases; D043323:alpha-Mannosidase; D007785:Lactose; D002118:Calcium; D005690:Galactose