Immediate elimination of labile HbA1c with allosteric effectors of hemoglobin.
Our aim was to find a simple method of removing labile glycosylated hemoglobin (HbA1c) from blood samples before it is measured by cation-exchange chromatography. Labile HbA1c is formed by the binding of glucose to the NH2-terminal valine of the beta-chain of HbA. We sought a more competitive binder for the same site to dissociate labile HbA1c to glucose and HbA. Inorganic phosphates were found to have a strong allosteric effect and a great ability to eliminate labile HbA1c. We developed our method with 4 mM tetrapolyphosphate in the hemolyzing solution to eliminate labile HbA1c during the automatic processing (at pH 6 and heated for 2 min at 45 degrees C) of blood samples for HbA1c estimation. This may be useful when estimating HbA1c by the manual method.
Nakashima
K
K
Department of Laboratory Medicine, St. Luke's College of Nursing, Tokyo, Japan.
Hattori
Y
Y
Yamazaki
K
K
Takechi
M
M
Andoh
Y
Y
Miyaji
T
T
D000494:Allosteric Regulation / Q000502:physiology; D000495:Allosteric Site; D002852:Chromatography, Ion Exchange; D004163:Diphosphoglyceric Acids / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D006442:Glycated Hemoglobin / Q000032:analysis* / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D010100:Oxygen / Q000378:metabolism; D010833:Phytic Acid / Q000494:pharmacology*; D011122:Polyphosphates / Q000494:pharmacology*
D004163:Diphosphoglyceric Acids; D005947:Glucose; D006442:Glycated Hemoglobin; D006454:Hemoglobins; D010100:Oxygen; D010833:Phytic Acid; D011122:Polyphosphates