[Comparative studies of the detection of Corynebacterium suis infections in swine by indirect immunofluorescence and culture].
In a comparative study 47 urine samples of sows and 9 swabs of preputial diverticulum of boars were investigated for detection of Corynebacterium suis (Eubacterium suis) using an indirect immunofluorescent technique, a Gram stained preparation, a direct culture technique and a culture technique after enrichment. Examinations show that the indirect immunofluorescent technique and the enriched culture technique provide the best results, but that the fluorescent technique saves time and expenses. Additionally in contrast to culture techniques the detection of C. suis is not affected by accompanying bacteria. Using an indirect immunofluorescence it is possible to detect antibody-coating of C. suis in urine sediment of sows as well. There were close correlations between evidence of antibody-coated C. suis and the presence of an ascending pyelonephritis.
Institut Für Mikrobiologie und Tierseuchen, Tierärztlichen Hochschule Hannover.
N
N
Langfeldt
M
M
Wendt
G
G
Amtsberg
D000818:Animals; D003352:Corynebacterium / Q000302:isolation & purification*; D003354:Corynebacterium Infections / Q000175:diagnosis / Q000662:veterinary*; D005260:Female; D005455:Fluorescent Antibody Technique; D008297:Male; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*
Vergleichende Untersuchungen zum Nachweis von Corynebacterium suis-Infektionen beim Schwein mit Hilfe der indirekten Immunfluoreszenz und der Kultur.