Presence of N6-methyladenine in GATC sequences of Bacillus popilliae and Bacillus lentimorbus KLN2.
Nine strains of Bacillus popilliae and Bacillus lentimorbus KLN2 contain N6-methyladenine in GATC sequences, as determined by using the restriction enzymes MboI and DpnI. Among eight other Bacillus species examined, all, except one strain of Bacillus brevis (ATCC 9999), lacked adenine methylation in GATC. A methylase with Escherichia coli dcm site specificity was not present in any of the Bacillus species studied.
Dingman
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Connecticut Agricultural Experiment Station, New Haven 06504.
D000225:Adenine / Q000031:analogs & derivatives* / Q000032:analysis; D001407:Bacillus / Q000032:analysis* / Q000235:genetics; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis*; D008745:Methylation; D013045:Species Specificity
D000225:Adenine; D004269:DNA, Bacterial; C000730567:6-methyladenine