Invasive migration of epidemic Kaposi's sarcoma cells in vitro.
Kaposi's sarcoma (KS) is a low grade malignant neoplasm which shows invasive growth and often occurs in immunosuppressed patients with the Acquired Immune Deficiency Syndrome (AIDS; epidemic KS). It is also found in elderly men where it is usually limited to the skin (classic KS). The present study investigated the chemotaxis and invasive migration of epidemic KS cells in vitro and compared them to cells grown from classic KS lesions and to fibroblasts. Epidemic KS cells demonstrated invasive migration through reconstituted basement membrane (Matrigel) as well as through interstitial connective tissue (collagen I) in early passages, whereas fibroblasts did not invade either barrier. Epidemic KS cells in late passages did not show any invasive migration. Following pretreatment with tumour necrosis factor alpha (TNF-alpha) there was no enhanced migration through the Matrigel and collagen I for epidemic KS cells, whereas classic KS cells showed an increased migration through the type I collagen barrier.
Dermatologische Klinik und Poliklinik, Ludwig-Maximilians-Universität München, F.R.G.
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Schirren
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Roth
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Hein
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Werner
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Krieg
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Braun-Falco
D001485:Basement Membrane; D002465:Cell Movement / Q000502:physiology; D002633:Chemotaxis / Q000502:physiology; D003238:Connective Tissue; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D008297:Male; D009361:Neoplasm Invasiveness; D012514:Sarcoma, Kaposi / Q000473:pathology*; D012878:Skin Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D014409:Tumor Necrosis Factor-alpha