Characterization by cDNA cloning of the mRNA for seminalplasmin, the major basic protein of bull semen.
A cDNA library derived from poly(A)+RNA of bull seminal vesicle tissue was screened with synthetic DNA probes specific for seminalplasmin (SAP), the major basic protein of bull semen. From a number of positive clones, pBSV12, containing a 577-bp insert, was identified and sequenced. The derived amino acid sequence comprises the known amino acid sequence of SAP with an amino terminal representing a putative signal sequence; at the carboxyl terminus the sequence contains an additional lysine residue. Present experimental data do not distinguish between two potential SAP precursor molecules, each starting with a methionine residue and differing by 10 amino acid residues in the leader peptide. Comparative Northern analysis reveals a SAP-specific mRNA of 700 bp, which lacks RNA from bovine testis as well as from seminal vesicle tissue of a bull calf; hence, expression of the SAP gene appears to be under androgen and/or developmental control. Southern analysis indicates that one gene appears to specify SAP. SAP-like DNA sequences were detected in ovine and porcine genomic DNA.
Max-Planck-Institut für biophysikalische Chemie, Göttingen, West Germany.
S
S
Wagner
J
J
Freudenstein
K H
KH
Scheit
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D015183:Restriction Mapping; D012661:Semen; D029607:Seminal Vesicle Secretory Proteins
D011506:Proteins; D029607:Seminal Vesicle Secretory Proteins; D004247:DNA