Demonstration of MHC class II-restricted cytotoxic T lymphocytes in mice against herpes simplex virus.
In humans CD4+ major histocompatibility complex (MHC) class II-restricted T cells dominate cytotoxic T-lymphocyte (CTL) responses to herpes simplex virus (HSV), whereas in the mouse only CD8+ MHC class I-restricted CTL have been reported. In this study, we demonstrate that a minor fraction (around 30%) of the response in draining lymph nodes of acute local HSV infections is attributable to CD4+ CTL mice. Such CTL were identified on the basis of antiserum inhibition studies, negative depletion approaches, as well as their differing antigen processing requirements to CD8+ MHC class I-restricted CTL. A possible role for CD4+ CTL as immunoregulators in local infections is discussed briefly.
Department of Microbiology, College of Veterinary Medicine, University of Tennessee, Knoxville 37996-0845.
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Kolaitis
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Doymaz
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Rouse
D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis; D002460:Cell Line; D002738:Chloroquine / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects / Q000276:immunology; D004640:Emetine / Q000494:pharmacology; D005260:Female; D006561:Herpes Simplex / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000943:Antigens, Differentiation; D000949:Histocompatibility Antigens Class II; D002738:Chloroquine; D004640:Emetine