Detection of cystathionine ketimine in bovine cerebellum.
A new sulfur-containing cyclic imino acid, cystathionine ketimine, has been detected in bovine cerebellum by gas chromatography, gas chromatography-mass spectrometry, and high pressure liquid chromatography procedures. Gas chromatography and gas-mass analyses are based on derivatization of endogenous cystathionine ketimine with diazomethane after a simple enrichment procedure. The high pressure liquid chromatography procedure takes advantage of the selective absorbance at 380 nm of the phenyl isothiocyanate-ketimine interaction product. The concentration of this new sulfur imino acid found in a pool of four bovine cerebella is approximately 0.5 nmol/g.
Ricci
G
G
Institute of Biochemical Sciences, University of Chieti G. D'Annunzio, Italy.
Vesci
L
L
Matarese
R M
RM
Antonucci
A
A
Maggio
A
A
Pecci
L
L
Cavallini
D
D
D000818:Animals; D002417:Cattle; D002531:Cerebellum / Q000378:metabolism*; D002849:Chromatography, Gas; D003540:Cystathionine / Q000031:analogs & derivatives* / Q000378:metabolism; D017879:Isothiocyanates; D013058:Mass Spectrometry; D009994:Osmolar Concentration; D013861:Thiocyanates
D017879:Isothiocyanates; D013861:Thiocyanates; C005441:phenylisothiocyanate; D003540:Cystathionine; C038681:cystathionine ketimine