DNA analysis of intracellular Campylobacter-like organisms associated with the porcine proliferative enteropathies: novel organism proposed.
Intracellular Campylobacter-like organisms are a consistent feature of the porcine proliferative enteropathies. The relationship between these organisms and known Campylobacter sp. previously associated with the disease was studied using restriction enzyme analysis and DNA-DNA blot hybridization techniques. BglII restriction enzyme fragment patterns of DNA of the Campylobacter-like organisms were fundamentally different from those of C. mucosalis, C. hyointestnalis, C. jejuni, and C. coli. Crude DNA preparations from Campylobacter-like organisms hybridized strongly with homologous preparations, weakly with porcine DNA and not at all with DNA from Campylobacter sp. Fragment specific DNA probes prepared from Campylobacter-like organisms only hybridized with homologous preparations. This work suggests that the intracellular Campylobacter-like organisms are not one of the known Campylobacter sp. It is possible that they are a novel, uncultured organism worthy of a new name, such as HC. intracellulare'.
McOrist
S
S
Department of Veterinary Pathology, Royal (Dick) School of Veterinary Studies, University of Edinburgh, U.K.
Lawson
G H
GH
Roy
D J
DJ
Boid
R
R
D000818:Animals; D001426:Bacterial Proteins; D015139:Blotting, Southern; D002167:Campylobacter / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D003106:Colon / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004751:Enteritis / Q000382:microbiology / Q000662:veterinary*; D007082:Ileum / Q000382:microbiology; D010957:Plasmids; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific