Sodium pyruvate inhibits the spontaneous release of 51Cr from RBC in chromium release assays.
This report describes the utility of sodium pyruvate for markedly decreasing the spontaneous release of chromium-51 from RBC in long term chromium release assays. This method can be used to increase the sensitivity and duration of cytotoxicity assays which use chromium-51-labeled RBC as targets.
Department of Microbiology and Immunology, University of Miami School of Medicine, FL 33101.
A S
AS
Colsky
J S
JS
Peacock
D000818:Animals; D002645:Chickens; D002857:Chromium / Q000378:metabolism*; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006461:Hemolysis; D007158:Immunologic Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D011773:Pyruvates / Q000494:pharmacology*; D019289:Pyruvic Acid
D011773:Pyruvates; D002857:Chromium; D019289:Pyruvic Acid