Developmental patterning of the carbohydrate antigen FC10.2 during early embryogenesis in the chick.
An oligosaccharide antigen (FC10.2), formerly described only in mammalian cells and secreted glycoproteins, has been detected and found to display striking temporal and spatial patterning in the chick during early embryonic development. This antigen is expressed on type 1 chains, which are isomers of oligosaccharides of the poly-N-acetyllactosamine series (type 2 chains). Immunoreactivities before and after neuraminidase treatment of serial sections of chick embryos during the first 17 stages of development indicate that the FC10.2 structure occurs predominantly in the sialylated form (S-FC10.2). The FC10.2 and S-FC10.2 antigens are prominent markers of the primordial germ cells, being strongly expressed by these cells from the pre-primitive streak stage onwards. S-FC10.2 is also a clear marker of the pronephric duct from its first appearance. Initially present over the entire apical surface of the ectoderm, antigenicity diminishes in an antero-posterior direction as neurulation proceeds. A unique pattern for a carbohydrate antigen is displayed by cells of the primitive streak; antigenicity is lost with de-epithelialisation and ingression, but is regained in a pericellular distribution on the mesoderm cells that emerge from the primitive streak. Thereafter, successive changes in expression and distribution of FC10.2 and S-FC10.2 are features of mesodermal tissues, particularly during somitogenesis. These antigens are prominent components of the extracellular matrix around the notochord and sclerotome cells. They are also prominent posteriorly in the subectodermal region, ceasing abruptly at the lateral limits of the embryo proper. Although no absolute correlations can yet be made, several features of the distribution of these antigens suggest that they may be integral components of, or ligands for, cell adhesion molecules.
Loveless
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Section of Glycoconjugate Research, Clinical Research Centre, Harrow, Middlesex, UK.
Bellairs
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R
Thorpe
S J
SJ
Page
M
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Feizi
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D000818:Animals; D000941:Antigens / Q000032:analysis*; D000943:Antigens, Differentiation / Q000032:analysis; D002642:Chick Embryo; D005455:Fluorescent Antibody Technique; D005775:Gastrula / Q000276:immunology; D005854:Germ Cells / Q000276:immunology*; D008648:Mesoderm / Q000276:immunology; D009844:Oligosaccharides / Q000276:immunology*
D000941:Antigens; D000943:Antigens, Differentiation; D009844:Oligosaccharides