Analysis of a mutated phage T6 receptor protein of Escherichia coli K 12.
The tsx-206 allele encodes an altered Tsx protein, Tsx-206, that can no longer function as the T6 receptor. We show here that this allele also confers resistance to the Tsx-specific phages H1, H3, H8, K9, K18 and Ox1 but not to colicin K. The Tsx-206 protein still mediates the efficient permeation of deoxyadenosine across the outer membrane at low substrate concentration. A host-range mutant of phage T6, T6h3.1, was isolated which can use both the Tsx-206 and the Tsx wild-type protein as its receptor. Cloning and DNA sequence analysis of the tsx-206 allele showed that the phage resistant phenotype was associated with an Asn to Tyr substitution at position 254 of the 272-residue Tsx protein.
Department of Biology, University of Konstanz, Federal Republic of Germany.
C
C
Maier
A
A
Middendorf
E
E
Bremer
D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D001692:Biological Transport; D003087:Colicins / Q000494:pharmacology; D003839:Deoxyadenosines / Q000235:genetics / Q000378:metabolism; D003841:Deoxycytidine / Q000235:genetics / Q000378:metabolism; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D011991:Receptors, Virus / Q000235:genetics*; D013604:T-Phages / Q000235:genetics*
D001425:Bacterial Outer Membrane Proteins; D003087:Colicins; D003839:Deoxyadenosines; D011991:Receptors, Virus; D003841:Deoxycytidine