A slide enzyme-linked immunosorbent assay (SELISA) for the diagnosis of Babesia bovis infections and for the screening of Babesia-specific monoclonal antibodies.
A slide enzyme-linked immunosorbent assay (SELISA), a modification of the standard ELISA technique, was developed for detection of Babesia bovis antibodies in bovine sera. Smears of B. bovis-infected blood were used as the source of antigen in the test which was read using a light microscope. Monoclonal antibodies to defined B. bovis antigens were used to demonstrate the cellular specificity of the test. The SELISA was shown to be as sensitive as existing non-enzyme based serological tests for B. bovis. Comparative to the conventional ELISA technique, it was more economical and technically simpler, thus making it an ideal test for field application.
Kung'u
M W
MW
Department of Parasitology, University of Queensland, St. Lucia, Australia.
Goodger
B V
BV
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000913:Antibodies, Protozoan / Q000032:analysis*; D001403:Babesia / Q000276:immunology*; D001404:Babesiosis / Q000175:diagnosis*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006386:Hemagglutination Tests
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan