Heat-labile phosphatase simplifies the preparation of dephosphorylated vector DNA.
A novel enzyme for DNA dephosphorylation, HK phosphatase, is completely and irreversibly inactivated at 65 degrees C. HK phosphatase treatment of BamHI-digested pBR322 reduces nonrecombinants in cloning experiments to approx. 5% of transformant colonies.
Hoffman
L M
LM
Epicentre Technologies, Madison, WI 53713.
Jendrisak
J
J
D000469:Alkaline Phosphatase / Q000494:pharmacology*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D006358:Hot Temperature; D010766:Phosphorylation; D010957:Plasmids / Q000187:drug effects*
D000469:Alkaline Phosphatase