Application of long synthetic oligonucleotides for gene analysis: effect of probe length and stringency conditions on hybridization specificity.
Two different lengths of long unique synthetic oligonucleotide probes (37- and 48-mers) specific for human major histocompatibility complex (MHC) class II beta genes were synthesized. These oligonucleotides were utilized to examine factors influencing hybridization specificity. Both probe length and stringency of washing conditions were found to be crucial factors for sequence-specific hybridization.
Kajimura
Y
Y
Department of Molecular Genetics, Beckman Research Institute of the City of Hope, Duarte, CA.
Krull
J
J
Miyakoshi
S
S
Itakura
K
K
Toyoda
H
H
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000032:analysis; D006683:HLA-DQ Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature
D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D015345:Oligonucleotide Probes; D004247:DNA