Expression of the 5.1 H11 antigen, a fetal muscle surface antigen, in normal and neoplastic tissue.
The monoclonal antibody 5.1 H11 recognizes an antigen on human fetal muscle and on rhabdomyosarcoma cell lines and xenografts that has been shown to be homologous to the neural cell adhesion molecule. To evaluate its range of expression, we used immunoperoxidase staining of fresh frozen-tissue sections to determine monoclonal antibody 5.1 H11 reactivity in normal and neoplastic tissue. Among normal tissue specimens, intense antibody staining was seen in brain and peripheral nerve, and weaker staining in ganglionic elements of colon. In addition to 26 of 29 rhabdomyosarcoma specimens, 5.1 H11 antibody showed reactivity to 9 of 10 Wilms' tumors, 6 of 6 neural tumors, and 4 of 4 gliomas, and with single specimens of ectomesenchymoma, clear-cell sarcoma of kidney, undifferentiated sarcoma of liver, ovarian fibroma, and neurofibroma. We conclude that the monoclonal antibody 5.1 H11 recognizes an antigen present not only on fetal muscle but on normal brain and nerve as well. In addition to rhabdomyosarcoma, a variety of other tumors, most of which have been previously shown to express neural cell adhesion molecule, also appear to express the antigen recognized by 5.1 H11. Our results thus offer additional confirmatory evidence that an epitope of neural cell adhesion molecule is the antigen for 5.1 H11.
Strother
D R
DR
Department of Hematology-Oncology, St Jude Children's Research Hospital, Memphis, TN 38101-0318.
Parham
D M
DM
Houghton
P J
PJ
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D015816:Cell Adhesion Molecules, Neuronal / Q000276:immunology; D002460:Cell Line; D005333:Fetus / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D009132:Muscles / Q000196:embryology / Q000276:immunology*; D012016:Reference Values; D012208:Rhabdomyosarcoma / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D015816:Cell Adhesion Molecules, Neuronal