Boar sperm membranes antigens. I. Topography of a mobile glycoprotein of the sperm cell membrane.
A monoclonal antibody, designated mAb P86/5, was generated by immunization of female Balb/c mice with a membrane vesicle fraction composed of the outer acrosomal membrane and plasma membrane (PM-OAM). As determined by fluorescence microscopy and electron microscopy P86/5 recognizes a sperm plasma membrane antigen that is restricted to the sperm head. In intact spermatozoa the P86/5-antigen is distributed over the surface of the sperm head with the exception of the rostral region. By comparing the antibody binding pattern generated at 4 degrees C and 25 degrees C, it could be shown that the P86/5-antigen is capable to diffuse freely within the cell membrane overlying the acrosome whereas its lateral mobility is restricted to the post-acrosomal region. The P86/5-antigen had a molecular weight of about 78 kDa as revealed by SDS-PAGE and western blotting. The glycoprotein nature of the P86/5-antigen was established by lectin affinity chromatography.
Töpfer-Petersen
E
E
Department of Dermatology, University of Munich, Federal Republic of Germany.
Friess
A E
AE
Stoffel
M
M
Schill
W B
WB
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis / Q000276:immunology; D005455:Fluorescent Antibody Technique; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis*; D008854:Microscopy, Electron; D013094:Spermatozoa / Q000032:analysis / Q000276:immunology / Q000648:ultrastructure*; D013552:Swine
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D008562:Membrane Glycoproteins