Stain analysis using oligonucleotide probes specific for simple repetitive DNA sequences.
The use of oligonucleotide fingerprinting is evaluated in practical forensic work, using both artificially and systematically produced stains as well as actual case work material. The probes (CAC5/(GTG)5 are superior because of their individualizing potential in comparatively fresh specimens with little DNA degradation, whereas (GACA)4, still produces substantial information when high molecular weight DNA is lacking. The overall limitations and the advantages of this technology are discussed in detail and compared to the classical minisatellite probes.
Institut für Gerichtliche Medizin der Humboldt-Universität zu Berlin, GDR.
L
L
Roewer
P
P
Nürnberg
E
E
Fuhrmann
M
M
Rose
O
O
Prokop
J T
JT
Epplen
D001801:Blood Stains; D004247:DNA / Q000032:analysis* / Q000097:blood / Q000652:urine; D016172:DNA Fingerprinting; D005260:Female; D006708:Homicide; D006801:Humans; D008297:Male; D009132:Muscles; D015345:Oligonucleotide Probes; D012091:Repetitive Sequences, Nucleic Acid; D012661:Semen; D014621:Vagina
D015345:Oligonucleotide Probes; D004247:DNA