Determination of 6-mercaptopurine and its metabolites in plasma or serum by high performance liquid chromatography.
A sensitive and accurate reversed phase liquid chromatographic assay was developed for the determination of 6-mercaptopurine (6MP) (the active metabolite of azathioprine) in human plasma. The assay involved extraction into acetonitrile and dichloromethane from plasma pretreated with 0.038 M of dithiothreitol solution. The residue was analyzed by isocratic chromatography on a C18 analytical column with UV detection at 326 nm. The average extraction recovery of 6MP was 85%. The method has been applied successfully to the determination of 6MP and its metabolites in pharmacokinetic studies.
Laboratoire de Pharmacologie Clinique, Hôpital Edouard Herriot, Lyon, France.
Z
Z
Sahnoun
F
F
Serre-Debeauvais
J
J
Lang
G
G
Faucon
M
M
Gavend
D001379:Azathioprine / Q000008:administration & dosage / Q000009:adverse effects; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D015122:Mercaptopurine / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet; D013439:Sulfhydryl Reagents
D013439:Sulfhydryl Reagents; D015122:Mercaptopurine; D001379:Azathioprine