Determination of nitrite in cured meats by ion-exclusion chromatography with electrochemical detection.
A rapid liquid chromatographic (LC) method was developed for a sensitive determination of nitrite in cured meats, using ion-exclusion chromatographic separation and electrochemical detection (IEC-EC). The current AOAC colorimetric method requires 2 h shaking in a steam bath to eliminate interference from reducing compounds such as ascorbic acid. In the present method, nitrite was analyzed in the presence of ascorbic acid without interference, and the extraction time was reduced to 1 min. The extracted nitrite was determined by ion chromatography using anion-exclusion/HS column and amperometric detector equipped with platinum or glassy carbon electrode operating at +1.0 V vs Ag/AgCl reference electrode. The detection limit was 1 ppb as NO2-. The recoveries of 50 ppm nitrite added to frankfurter and meat stick were 103 and 99.6%, respectively, with relative standard deviations less than 4%. The high speed, sensitivity, and selectivity make the new method a useful alternative to the AOAC colorimetric method.
U.S. Army Natick Research, Development and Engineering Center, Food Engineering Directorate, Natick, MA 01760.
H J
HJ
Kim
K R
KR
Conca
D000818:Animals; D001205:Ascorbic Acid / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002852:Chromatography, Ion Exchange; D004563:Electrochemistry; D007202:Indicators and Reagents; D008460:Meat / Q000032:analysis*; D009573:Nitrites / Q000032:analysis*
D007202:Indicators and Reagents; D009573:Nitrites; D001205:Ascorbic Acid