[Spontaneous synthesis of colicin].
We studied regularities of the spontaneous synthesis of colicin E1 and its regulatory role in the survival of each cell and the population. For the first time, colicin from spontaneously induced cells was isolated and characterized, and the kinetics of its synthesis determined in a separate cell. It was stated that the time of colicin accumulation in an induced cell and the half-time period of a similar cell exceeds the period of cell doubling by 1.5 and 2 times, respectively. Spontaneously synthesized colicin was shown not to differ from the colicin synthesized by using inductors of the SOS-system of cell reparation. A conception was formulated of a biological system containing both colicinogenic cells with plasmids with encoded proteins of colicin, immunity and lysis on them and competitive plasmid-free cells sensitive or resistant to colicin. Environment dependent colicin synthesis in the system shown is regarded as regulator functioning according to the feed-back principle.
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Malkhos'ian
D002846:Chromatography, Affinity; D003087:Colicins / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D005798:Genes, Bacterial; D007700:Kinetics; D010957:Plasmids
D003087:Colicins
Spontannyĭ sintez kolitsina.