[Diagnosis of HIV by clinical pathological methods].
HIV (human immunodeficiency virus)-I infection can be detected by the presence of HIV antigen, antibody to HIV, or gene of HIV itself in patient's blood. There are a considerable number of such antibody detecting methods (first generation) commercially available, but even the first was introduced in only mid-1985. These tests detect antibody in more than 90% of patients with confirmed AIDS. Recently, new antibody detecting methods (second generation) was produced using a single antigen (e.g. p 24, gp 41) of HIV-I by recombinant DNA or synthetic peptide. Furthermore, detection methods for DNA or RNA of HIV were developed and these methods are introduced in clinical use in near future.
Department of Clinical Laboratory, Omiya Medical Center Jichi Medical School.
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Sakurabayashi
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Matsuda
D015492:AIDS Serodiagnosis; D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis*; D015153:Blotting, Western; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D006678:HIV / Q000235:genetics / Q000276:immunology; D015483:HIV Antibodies / Q000032:analysis; D015488:HIV Antigens / Q000032:analysis; D006801:Humans; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests
D004279:DNA, Viral; D015483:HIV Antibodies; D015488:HIV Antigens