Kinetics of hemolysin production in bovine group B streptococci.
Thirteen strains of group B streptococci etiologically related to bovine infections were investigated in order to observe the effects of non-hemoglobin iron and glucose on growth and hemolysin production; and to determine the necessity to stabilize the hemolysin with a carrier-stabilizer agent. Ferric citrate was diluted to give final concentrations of 1-11 micrograms/ml, added to iron-reduced (by CaCl2 precipitation) brain heart infusion media, inoculated and incubated at 37 degrees C. Parallel experiments were carried out with glucose. A variety of agents was employed to act as hemolysin stabilizers. Hemolysin production was detected by lysis of sheep erythrocytes. Both iron and glucose were crucial in concentrations 5-7 micrograms/ml and 0.5-1% respectively. Bovine serum albumin-starch mixture acted as an excellent stabilizer.
Griffiths
B B
BB
Oral Roberts University, School of Medicine, City of Faith Medical and Research Center, Tulsa, Oklahoma 74137.
D000818:Animals; D002417:Cattle / Q000382:microbiology*; D005947:Glucose / Q000494:pharmacology; D006460:Hemolysin Proteins / Q000096:biosynthesis*; D007501:Iron / Q000032:analysis; D007700:Kinetics; D013292:Streptococcus agalactiae / Q000254:growth & development / Q000378:metabolism*
D006460:Hemolysin Proteins; D007501:Iron; D005947:Glucose