Anomalous gel migration of DNA oligomers containing multiple conformational junctions.
We have previously shown that a short 16 base pair DNA oligomer can accommodate a B-Z conformational junction [Sheardy, R. D., & Winkle, S. A. (1989) Biochemistry 28, 720-725]. Results from 1H NMR studies indicated that only three base pairs were involved in the junction and that one of these base pairs was highly distorted. Being interested in the nature of this distortion, we constructed DNA oligomers which have the potential to contain multiple B-Z junctions for polyacrylamide electrophoretic studies. We report that the mobilities displayed by these molecules through acrylamide gels in the absence and presence of cobalt suggest that these molecules run shorter than they actually are. This anomalous migration may be due to structural/dynamic properties of the DNA helix manifested by the periodic distortions of the potential B-Z junctions.
Winkle
S A
SA
Department of Chemistry, Florida International University, Miami 33199.
Sheardy
R D
RD
D001483:Base Sequence; D002942:Circular Dichroism; D003035:Cobalt; D004247:DNA / Q000302:isolation & purification* / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000302:isolation & purification*
D009838:Oligodeoxyribonucleotides; D003035:Cobalt; D004247:DNA