Plasmatic antiproteinase activity enhancement by insoluble functionalized polystyrene surfaces.
Antithrombogenic functional polymer surfaces have been obtained by grafting heparin or by substituting insoluble polystyrene with sulphonate and/or amino acid sulphamide groups. Their heparin-like properties have been related to their catalytic effects on the antithrombin III - thrombin complex formation. Amongst these antithrombogenic surfaces, this study demonstrates that some insoluble amino acid sulphamide derivatives of polystyrene strongly potentiate heparin cofactor II, in addition to antithrombin III. In contrast, an insoluble polystyrene sulphonate and, to a lesser extent, an insoluble heparin copolymer, are better catalysts of antithrombin III. It is hypothesized that such different behaviours result from different conformations of the species adsorbed onto the surfaces. The conclusions support the possible use of such amino acid sulphamide groups to prepare antithrombogenic surfaces in contact with blood.
Charef
S
S
Laboratoire de Recherches sur les Macromolécules, CNRS UA 502, Université Paris-Nord, Villetaneuse, France.
Jozefowicz
M
M
Labarre
D
D
Tapon-Bretaudiere
J
J
Fischer
A M
AM
Bros
A
A
D000990:Antithrombin III / Q000378:metabolism; D001672:Biocompatible Materials; D015844:Heparin Cofactor II / Q000378:metabolism; D066298:In Vitro Techniques; D011137:Polystyrenes / Q000737:chemistry*; D011480:Protease Inhibitors / Q000097:blood*; D013499:Surface Properties; D013923:Thromboembolism / Q000517:prevention & control*
D001672:Biocompatible Materials; D011137:Polystyrenes; D011480:Protease Inhibitors; D015844:Heparin Cofactor II; D000990:Antithrombin III