Pseudohypophosphataemia as a result of bilirubin interference.
During the routine use of a discrete analyser it was noted that, when the serum bilirubin concentration was greater than 50 mumol/L, there was interference with serum phosphate determination measured by the formation of unreduced phosphomolybdate using a bichromatic system of measurement. The degree of interference was assessed by comparison with a reduced phosphomolybdate method (molybdenum blue). The interference cannot be removed by changing the secondary wavelength or by the use of a sample blank. It is proportional to the serum bilirubin concentration, but is not significant when this is less than 50 mumol/L. The monochromatic non-reduced phosphomolybdate method compares well with the reduced method.
Department of Clinical Chemistry, King's Mill Hospital, Sutton-in-Ashfield, Nottinghamshire UK.
G O
GO
Duncanson
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HG
Worth
D001663:Bilirubin / Q000097:blood / Q000378:metabolism*; D006801:Humans; D007565:Jaundice / Q000175:diagnosis; D008982:Molybdenum; D010710:Phosphates / Q000097:blood*; D010756:Phosphoric Acids; D012680:Sensitivity and Specificity; D013053:Spectrophotometry
D010710:Phosphates; D010756:Phosphoric Acids; D008982:Molybdenum; D001663:Bilirubin; C003125:phosphomolybdic acid