[Separation and comparative characteristics of subunits of phenylalanyl-tRNA synthetase from Escherichia coli MRE-600 and Thermus thermophilus HB8].
Separation of alpha- and beta-subunits of phenylalanyl-tRNA-synthetases from E. coli MRE-600 and Thermus thermophilus HB8 using FPLC has been carried out for the first time. The separated subunits of both enzymes do not possess any detectable tRNA-amino-acylation activity. It was found that in the case of the E. coli enzyme beta-subunits exist in solution mainly in the monomeric form with negligible formation of beta 2-dimers, while alpha-subunits predominantly form alpha 2-dimers over the same concentration range. In the case of Thermus thermophilus phenylalanyl-tRNA-synthetase, both alpha- and beta-subunits mainly exist in the dimeric form. The putative mechanisms of alpha-subunit aggregation and of subunit association for alpha 2 beta 2-type enzymes are discussed.
E V
EV
Bobkova
A D
AD
Vol'fson
V N
VN
Ankilova
O I
OI
Lavrik
D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D046911:Macromolecular Substances; D008970:Molecular Weight; D010652:Phenylalanine-tRNA Ligase / Q000302:isolation & purification*; D013045:Species Specificity; D013824:Thermus / Q000201:enzymology*
D046911:Macromolecular Substances; D000604:Amino Acyl-tRNA Synthetases; D010652:Phenylalanine-tRNA Ligase
Razdelenie i sravnitel'naia kharakteristika sub''edinits fenilalanil-tRNK-sintetaz iz Escherichia coli MRE-600 i Thermus thermophilus HB8.