Problems of [S-methyl-11C]-L-methionine as a protein synthesis marker in the pancreas.
To evaluate the possibility of [S-methyl-11C]-L-methionine as a protein synthesis marker in the pancreas, the effect of various labeling positions in the accumulation and metabolism of 14C-labeled L-methionines (S-methyl-14C, 1-14C and 3,4-14C) was studied. In mouse biodistribution studies, the methionines showed differing patterns of labeling position-dependent pancreatic accumulation. In the case of [S-methyl-14C]-L-methionine, protein-incorporation and methyl-transformation equally served as retention mechanisms in the pancreas, indicating [S-methyl-11C]-L-methionine's unsuitability as a pancreatic protein synthesis marker. For such purposes, [1-11C]-L-methionine is considered more suitable.
Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Y
Y
Fujibayashi
K
K
Kawai
Y
Y
Yonekura
K
K
Matsumoto
J
J
Konishi
A
A
Yokoyama
D000818:Animals; D002250:Carbon Radioisotopes; D008297:Male; D008715:Methionine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D051379:Mice; D010179:Pancreas / Q000378:metabolism*; D014176:Protein Biosynthesis; D014018:Tissue Distribution
D002250:Carbon Radioisotopes; D008715:Methionine; C038344:methionine methyl ester