Subchronic (13-week) oral toxicity of neohesperidin dihydrochalcone in rats.
Neohesperidin dihydrochalcone was administered to groups of 20 male and 20 female Wistar rats at dietary levels of 0, 0.2, 1.0 and 5.0% for 91 days. No treatment-related ophthalmoscopical, haematological or histopathological effects were observed. In the high-dose group, a marked caecal enlargement occurred in both sexes, accompanied by soft stools in the early stages of the study, somewhat lower plasma urea concentrations and increased plasma alkaline phosphatase activity and a decreased urinary pH. This group also showed slight growth depression accompanied by transient reduction in food intake; in males the body weights remained relatively low throughout the experimental period. Furthermore, bilirubin level was increased in females and total protein level was decreased in males of the high-dose group. The above changes were considered adaptive responses or chance effects rather than manifestations of clear toxicity. The low-and intermediate- dose groups did not show any compound-related untoward effect. It was concluded that the intermediate dose, providing an overall intake of about 750 mg neohesperidin dihydrochalcone per kg body weight per day, was the no-effect level.
TNO-CIVO Toxicology and Nutrition Institute, Zeist, The Netherlands.
B A
BA
Lina
H C
HC
Dreef-van der Meulen
D C
DC
Leegwater
D000284:Administration, Oral; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001663:Bilirubin / Q000097:blood; D001798:Blood Proteins / Q000032:analysis; D001835:Body Weight / Q000187:drug effects; D002432:Cecum / Q000187:drug effects; D002599:Chalcone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000633:toxicity; D047188:Chalcones; D004326:Drinking / Q000187:drug effects; D004355:Drug Stability; D004435:Eating / Q000187:drug effects; D005260:Female; D006569:Hesperidin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000633:toxicity; D008297:Male; D009929:Organ Size / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D011919:Rats, Inbred Strains; D013549:Sweetening Agents / Q000008:administration & dosage / Q000633:toxicity*; D014508:Urea / Q000097:blood
D001798:Blood Proteins; D047188:Chalcones; D013549:Sweetening Agents; C013613:neohesperidin dihydrochalcone; D002599:Chalcone; D014508:Urea; D006569:Hesperidin; D000469:Alkaline Phosphatase; D001663:Bilirubin