Utilization of beet molasses for sterol production by some moulds.
Different moulds were cultivated in beet molasses (BM)-containing medium. Penicillium crustosum Thom was superior to the other moulds in total sterols production (4% on dry weight basis), efficiency of convertibility of the BM sugars to sterols (2%), total lipids (19.4%) and unsaponified lipids (13.4%). The treatment of BM with H2SO4- followed by centrifugation allowed maximum fermentation yields. The highest unsaponified lipids (16.5%) and total sterols level (7.4%) were obtained with a medium composed of (g/1): NaNO3, 3; K2HPO4, 3; MgSO4.7H2O, 1.5; K2SO4, 0.11; ZnSO4.7H2O, 0.05; FeCl3.6H2O, 0.16; H2SO4-treated BM, 60. Maximal sterol yields (8.4%) and high growth rate were achieved at the accelerated growth phase (8 days old cultures), when the initial pH value of the medium was adjusted to 7.0.
Botany Department, Faculty of Science, Alex. Uni., Egypt.
K M
KM
Ghanem
N B
NB
Ghanem
A H
AH
el-Refai
D050260:Carbohydrate Metabolism; D003470:Culture Media; D005285:Fermentation; D005658:Fungi / Q000254:growth & development / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000096:biosynthesis; D008670:Metals / Q000032:analysis; D008828:Microbiological Techniques; D008965:Molasses; D010407:Penicillium / Q000254:growth & development / Q000378:metabolism; D013261:Sterols / Q000096:biosynthesis*
D003470:Culture Media; D008055:Lipids; D008670:Metals; D013261:Sterols