The 5'-leader sequence of tobacco mosaic virus RNA mediates initiation-factor-4E-independent, but still initiation-factor-4A-dependent translation in yeast extracts.
Messenger RNAs encoding chloramphenicol acetyltransferase (CAT) with or without the 5'-leader sequence of tobacco mosaic virus (TMV) RNA were synthesized in vitro and translated in Saccharomyces cerevisiae extracts dependent on eukaryotic initiation factors eIF-4E or eIF-4A. The 5'-leader sequence of TMV RNA renders translation of CAT mRNA eIF-4E-independent but still 4A-dependent.
Institut für Biochemie und Molekularbiologie, Universität Bern, Switzerland.
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M
Altmann
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S
Blum
T M
TM
Wilson
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Trachsel
D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics*; D039601:Eukaryotic Initiation Factor-4A; D039561:Eukaryotic Initiation Factor-4E; D008715:Methionine / Q000378:metabolism; D010448:Peptide Initiation Factors / Q000378:metabolism*; D014176:Protein Biosynthesis; D021382:Protein Sorting Signals / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014027:Tobacco Mosaic Virus / Q000235:genetics*
D039561:Eukaryotic Initiation Factor-4E; D010448:Peptide Initiation Factors; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D012367:RNA, Viral; D008715:Methionine; D015500:Chloramphenicol O-Acetyltransferase; D039601:Eukaryotic Initiation Factor-4A