Isolation of Candida tropicalis auxotrophic mutants.
An enrichment scheme using nystatin was designed for the isolation of auxotrophic mutants from the diploid-alkane-utilizing yeast Candida tropicalis. A collection of 194 auxotrophs representing 7 phenotypes was isolated. One class of mutants was identified as having a defect in histidinol dehydrogenase activity and a second class of mutants was identified as having a defect in orotidine monophosphate decarboxylase activity. These strains are good candidates to be carrying mutations corresponding to the HIS4 and URA3 genes of Saccharomyces cerevisiae.
Salk Institute Biotechnology/Industrial Associates, Inc., La Jolla, California 92037.
M A
MA
Gleeson
L O
LO
Haas
J M
JM
Cregg
D000429:Alcohol Oxidoreductases / Q000235:genetics; D002175:Candida / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D005819:Genetic Markers; D009154:Mutation; D009172:Mycology / Q000379:methods; D009761:Nystatin; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013045:Species Specificity
D005819:Genetic Markers; D009761:Nystatin; D000429:Alcohol Oxidoreductases; C021358:histidinol dehydrogenase; D009964:Orotidine-5'-Phosphate Decarboxylase