Calcium-dependent anticandidal action of pradimicin A.
Pradimicin A shows candicidal activity at 10 micrograms/ml in vitro. The action of pradimicin A on Candida albicans cells involves a set of specific cell surface interactions in a Ca2(+)-dependent manner. These include binding to the mannan components on the cell surface and subsequent interactions at the level of the plasma membrane, causing K+ leakage and cell death. The protoplasts prepared from C. albicans undergo lysis rapidly when treated with pradimicin A. These results suggest that pradimicin A acts primarily on the candidal plasma membrane, leading to a perturbation of membrane function.
Bristol-Myers Research Institute, Ltd., Tokyo Research Center, Japan.
Y
Y
Sawada
K
K
Numata
T
T
Murakami
H
H
Tanimichi
S
S
Yamamoto
T
T
Oki
D018943:Anthracyclines; D000903:Antibiotics, Antineoplastic / Q000378:metabolism / Q000494:pharmacology*; D000935:Antifungal Agents / Q000378:metabolism / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects; D003470:Culture Media; D004271:DNA, Fungal / Q000096:biosynthesis / Q000187:drug effects; D005656:Fungal Proteins / Q000096:biosynthesis / Q000187:drug effects; D011523:Protoplasts / Q000378:metabolism; D012331:RNA, Fungal / Q000096:biosynthesis / Q000187:drug effects; D000095643:Pradimicins and Benanomicins
D018943:Anthracyclines; D000903:Antibiotics, Antineoplastic; D000935:Antifungal Agents; D002118:Calcium; D003470:Culture Media; D004271:DNA, Fungal; D005656:Fungal Proteins; D012331:RNA, Fungal; C058036:pradimicin A; D000095643:Pradimicins and Benanomicins