Biosynthesis of the 25-kDa protein in the macrogametes/zygotes of Plasmodium falciparum.
Synthesis of the 25-kDa protein in the early midgut stages of Plasmodium falciparum was studied, using metabolic inhibitors (colchicine and actinomycin D) and pulse-labeling experiments. Experiments with colchicine showed that, immediately after induction of macrogametogenesis, 25-kDa protein synthesis occurs in both fertilized and nonfertilized macrogametes. The amount of 25-kDa protein synthesized increased slowly during time. Experiments with actinomycin D revealed that the slow increase of synthesis may be dependent on de novo messenger RNA synthesis.
Institute of Medical Parasitology, University of Nijmegen, The Netherlands.
H C
HC
Fries
M B
MB
Lamers
J
J
van Deursen
T
T
Ponnudurai
J H
JH
Meuwissen
D000818:Animals; D001345:Autoradiography; D003078:Colchicine / Q000494:pharmacology; D004247:DNA / Q000235:genetics; D003609:Dactinomycin / Q000494:pharmacology; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D008970:Molecular Weight; D010963:Plasmodium falciparum / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D015800:Protozoan Proteins / Q000096:biosynthesis*; D014158:Transcription, Genetic / Q000187:drug effects
D015800:Protozoan Proteins; D003609:Dactinomycin; D004247:DNA; D003078:Colchicine