Immunohistochemical analysis of bone morphogenetic protein (BMP) in osteosarcoma.
The monoclonal antibody against bovine bone morphogenetic protein (bBMP-McAb) was first used for demonstration of bone morphogenetic protein (BMP) in osteosarcoma. The avidin-biotin complex method (ABC) demonstrated that of the 18 osteosarcomas, 15 stained positive, while all 6 fibrosarcomas were negative. The results showed that BMP mainly exists in the tumor cell plasma and some tumor-like bone tissues. Using this staining method, we can not only differentiate osteosarcoma from fibrosarcoma and other non-bone-derived tumors, but also classify osteosarcoma according to the content and distribution of BMP and the patient's clinical situation, thus providing a scientific basis for clinical treatment.
Department of Oral Pathology, Stomatological College, Fourth Military Medical University, Xian, China.
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Jin
L J
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Yang
D000293:Adolescent; D000328:Adult; D019485:Bone Morphogenetic Proteins; D003937:Diagnosis, Differential; D005260:Female; D005354:Fibrosarcoma / Q000032:analysis*; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008339:Mandibular Neoplasms / Q000032:analysis*; D008441:Maxillary Neoplasms / Q000032:analysis*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis*; D012516:Osteosarcoma / Q000032:analysis*; D011506:Proteins / Q000032:analysis*
D019485:Bone Morphogenetic Proteins; D009363:Neoplasm Proteins; D011506:Proteins