Evidence of widespread binding of HLA class I molecules to peptides.
We have tested the binding of HLA class I proteins to peptides using a solid-phase binding assay. We tested 102 peptides, mostly derived from the HIV gag and HIV pol sequences. Most peptides did not bind to any class I protein tested. The pattern of binding among the three class I proteins tested, HLA-A2, -B27, and -B8, was approximately 85% concordant. Further, all five of the known HIV-1 gag T cell epitopes detected by human CTL bound at least one class I protein. Binding of class I to the peptides could be detected either by directly iodinated class I proteins, or indirectly using monoclonal antibodies specific for class I. The binding to the plates could be blocked with MA2.1, which binds in the alpha 1 region of A2, but not by W6/32, which binds elsewhere. The data presented here show that binding of class I to peptides is specific, but that many peptides bind to more than a single class I protein.
Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, United Kingdom.
J A
JA
Frelinger
F M
FM
Gotch
H
H
Zweerink
E
E
Wain
A J
AJ
McMichael
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D002460:Cell Line; D015683:Gene Products, gag / Q000276:immunology; D015684:Gene Products, pol / Q000276:immunology; D006678:HIV / Q000276:immunology; D015497:HIV-1 / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D011485:Protein Binding
D000911:Antibodies, Monoclonal; D015683:Gene Products, gag; D015684:Gene Products, pol; D015395:Histocompatibility Antigens Class I; D010446:Peptide Fragments