Alpha-amylase (EC 3.2.1.1) susceptibility rather than viscosity or gastric emptying rate controls plasma responses to starch in healthy humans.
The relationship between starch alpha-amylase (EC 3.2.1.1) susceptibility, plasma responses and gastric emptying rates has been investigated in humans. Nine randomly chosen healthy subjects were given three carbohydrate test meals (25 g starch or equivalent glucose units): two maize starch pastes with (a) 240 (S24) or (b) 500 (S50) g amylose/kg, and a glucose solution (GS). At 30 min, in vitro starch alpha-amylolysis was 48 (SD 4)% for S24 and 35 (SD 4)% for S50. Test meals differed in viscosity (mPa x s: S24, 54,000; S50, 190; GS, 4). Carbohydrates were labelled with 99mTechnetium and isotope gastric emptying was measured by external gamma counting. Carbohydrate isotopic gastric emptying patterns were exponential. Half gastric emptying time (min) was significantly (P less than 0.05) shorter for S50 (19(SD 2] than for GS (26(SD 2] or S24 (29(SD 2]. No correlation was found between half gastric emptying time and plasma response values. Values for peak insulin (pmol/l) above fasting were significantly (P less than 0.05) different: GS, 306 (SD 11); S24, 227 (SD 11); S50, 187 (SD 11). It is concluded that alpha-amylase susceptibility of the test carbohydrates is a determining factor in the insulin response of healthy subjects, while viscosity of the test meals and gastric emptying rate have no effect.
Bornet
F R
FR
Institut National de la Recherche Agronomique, Laboratoire de technologie appliquée à la nutrition, Nantes, France.
Bizais
Y
Y
Bruley des Varannes
S
S
Pouliquen
B
B
Delort Laval
J
J
Galmiche
J P
JP
D000328:Adult; D001786:Blood Glucose / Q000378:metabolism*; D005260:Female; D005746:Gastric Emptying / Q000502:physiology*; D006801:Humans; D006868:Hydrolysis; D066298:In Vitro Techniques; D007328:Insulin / Q000097:blood*; D007700:Kinetics; D008297:Male; D013213:Starch / Q000378:metabolism*; D014783:Viscosity; D000516:alpha-Amylases / Q000378:metabolism*
D001786:Blood Glucose; D007328:Insulin; D013213:Starch; D000516:alpha-Amylases