Effect of Fluosol-DA 20% and oxygen on response of C57BL/6 mice to whole-body irradiation.
Normal tissue effects in mice due to combinations of a perfluorochemical emulsion, Fluosol-DA 20%, 100% oxygen, and whole-body irradiation were investigated. Eight-to-10-week-old C57BL/6 male mice were injected via the tail vein with 10 ml/kg of Fluosol-DA with and without subsequent exposure to oxygen for 60 minutes. Animals then received graded doses of whole-body radiation (4 MV photons) at a dose rate of 2.85 +/- .015 Gy/minute. Using linear regression analysis, the lethal doses of radiation to 50% and 10% of the animals within 30 days in the absence of Fluosol-DA and oxygen were 8.35 Gy (95% c.l.:7.77-8.93 Gy) and 6.73 Gy (95% cl.:6.21-7.25 Gy), respectively, and were unaffected by Fluosol-DA and/or oxygen pre-treatment. However, Fluosol-DA given alone or in combination with oxygen produced increased balding and decreased graying incidence in mice within 60 days, and resulted in depressed weight gain 15 to 60 days post-treatment. Normal tissue effects due to administration of Fluosol-DA and oxygen in combination with whole-body irradiation have been demonstrated but appear minimal compared to other anti-tumor modalities currently under investigation.
Department of Radiation Oncology, Cooper Hospital/University Medical Center, Camden, NJ.
S M
SM
Waldow
R A
RA
Lustig
E L
EL
Brass-Marlow
M P
MP
Nunno
R J
RJ
Holst
P E
PE
Wallner
D000505:Alopecia / Q000209:etiology; D000818:Animals; D001835:Body Weight / Q000187:drug effects / Q000528:radiation effects; D004338:Drug Combinations; D005466:Fluorocarbons / Q000494:pharmacology*; D006200:Hair Color / Q000187:drug effects / Q000528:radiation effects; D006895:Hydroxyethyl Starch Derivatives; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010100:Oxygen / Q000494:pharmacology*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D014916:Whole-Body Irradiation
D004338:Drug Combinations; D005466:Fluorocarbons; D006895:Hydroxyethyl Starch Derivatives; D011838:Radiation-Sensitizing Agents; C017323:glucose, glycerol, hydroxyethyl starch, perfluorodecalin, perfluorotripropylamine, pluronic F-68, salts, yolk phospholipids drug combination; D010100:Oxygen