Elimination of ColE1 group (pBR322 and pBR329) plasmids in Escherichia coli by alpha-santonin.
alpha-Santonin, a compound extracted from the flower heads of Arthemisia maritima plant, was effective in elimination of small, multicopy, relaxed plasmids (pBR322 and pBR329 with CoLE1 origin of replication in Escherichia coli, whereas plasmids of IncF1, H1 and X group were totally refractory under similar conditions, suggesting that this agent was specific in curing the CoLE1 group of plasmids.
Department of Microbiology, Osmania University, Hyderabad, India.
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Bharathi
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Polasa
D003086:Bacteriocin Plasmids / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D006863:Hydrogen-Ion Concentration; D010957:Plasmids / Q000187:drug effects*; D012500:Santonin / Q000494:pharmacology*
D012500:Santonin