Identification, purification, and biological characterization of hematopoietic stem cell factor from buffalo rat liver--conditioned medium.
We have identified a novel growth factor, stem cell factor (SCF), for primitive hematopoietic progenitors based on its activity on bone marrow cells derived from mice treated with 5-fluorouracil. The protein was isolated from the medium conditioned by Buffalo rat liver cells. It is heavily glycosylated, with both N-linked and O-linked carbohydrate. Amino acid sequence following removal of N-terminal pyroglutamate is presented. The protein has potent synergistic activities in semisolid bone marrow cultures in conjunction with colony-stimulating factors. It is also a growth factor for mast cells. In two companion papers, we present the sequences of partial SCF cDNAs, identify SCF as a c-kit ligand, and map the SCF gene to the Sl locus of the mouse.
AMGEN Inc., AMGEN Center, Thousand Oaks, California 91320.
K M
KM
Zsebo
J
J
Wypych
I K
IK
McNiece
H S
HS
Lu
K A
KA
Smith
S B
SB
Karkare
R K
RK
Sachdev
V N
VN
Yuschenkoff
N C
NC
Birkett
L R
LR
Williams
D000595:Amino Acid Sequence; D000818:Animals; D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003470:Culture Media; D005472:Fluorouracil / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D016298:Hematopoietic Cell Growth Factors / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D008099:Liver / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D051381:Rats; D011915:Rats, Inbred BUF
D003470:Culture Media; D016298:Hematopoietic Cell Growth Factors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D005472:Fluorouracil