Coding region structure of interleukin-8 gene of human lung giant cell carcinoma LU65C cells that produce LUCT/interleukin-8: homogeneity in interleukin-8 genes.
A 1.9-kb fragment containing an interleukin-8 (IL-8) coding region was amplified by the polymerase chain reaction (PCR) from the genomic DNA of human lung giant cell carcinoma LU65C cells that produce LUCT/IL-8 with N-terminal sequence of AVLPR. The coding region was found to consist of 4 exons and 3 introns as identical as that of the gene of MDNCF/IL-8 lacking N-terminal AVLPR. PCR using genomic DNAs from human polymorphonuclear leukocytes and mononuclear cells also provided the same 1.9-kb fragment as that from LU65C genomic DNA. Thus, it seems likely that human cells possess IL-8 genes with the homogeneous coding region so that they may first produce the same mature protein with N-terminal AVLPR (= LUCT) which was then truncated.
Department of Antibiotics, National Institute of Health, Tokyo, Japan.
K
K
Hotta
K
K
Hayashi
J
J
Ishikawa
M
M
Tagawa
K
K
Hashimoto
S
S
Mizuno
K
K
Suzuki
D000595:Amino Acid Sequence; D001483:Base Sequence; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D002630:Chemotactic Factors / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D005796:Genes; D006801:Humans; D016209:Interleukin-8; D007378:Interleukins / Q000235:genetics*; D007962:Leukocytes / Q000032:analysis; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011499:Protein Processing, Post-Translational; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured / Q000032:analysis
D002630:Chemotactic Factors; D004273:DNA, Neoplasm; D016209:Interleukin-8; D007378:Interleukins; D009363:Neoplasm Proteins