title: Synthesis and characterization of a new substrate of Photinus pyralis luciferase: 4-methyl-D-luciferin.
abstract: A derivative of luciferin, 4-methyl-D-luciferin, was synthesized in low yield, and characterized by its ultraviolet and fluorescence spectra. The spectra of luciferin and its derivative are very similar. 4-Methyl-D-luciferin is a substrate of Photinus pyralis luciferase, and it causes light emission in luminometric assays.
authors.affiliation: CEMAGREF, Laboratoire de Biochimie, Lyon, France.
authors.forename: C
authors.initials: C
authors.lastname: Farace
authors.forename: B
authors.initials: B
authors.lastname: Blanchot
authors.forename: D
authors.initials: D
authors.lastname: Champiat
authors.forename: P
authors.initials: P
authors.lastname: Couble
authors.forename: G
authors.initials: G
authors.lastname: Declercq
authors.forename: J L
authors.initials: JL
authors.lastname: Millet
mesh_terms: D000818:Animals; D049454:Firefly Luciferin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D007202:Indicators and Reagents / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D007313:Insecta; D008156:Luciferases / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D007202:Indicators and Reagents; C066305:4-methylluciferin; D049454:Firefly Luciferin; D008156:Luciferases