[Fundus video fluorescein angiography with low steady light].
To decrease both the intensity of the exciting light and the amount of sodium fluorescein, we attached a compact image intensifier incorporating a microchannel plate with a fundus photoscope and tried to record video-fluorescein angiography with low steady light. We preliminarily examined the relationships of exciting and emitted light intensity with various concentrations of sodium fluorescein. The strongest fluorescence was obtained with a concentration of 0.01 mg/ml solution of sodium fluorescein. When the compact image intensifier was attached to the equipment, the exciting light intensity could be decreased to 1/300 with an exciting filter of lambda max of 486 nm. When sodium fluorescein of 0.01 mg/ml solution was injected directly into the heart of Rana catesbeiana, complete video-fluorescein angiography with sufficient brightness and resolution was possible. With the same machine, we recorded video-fluorescein angiography in a normal human subject with injection of 5 ml of 10% sodium fluorescein. The ordinary observing light of the machine was strong enough for video-recording. This exciting light intensity caused no trouble and elicited no complaint from the examinee. These results showed that the use of a compact image intensifier incorporated microchannel plate with a fundus photoscope made it possible to record video-fluorescein angiography and to decrease exciting light intensity and the amount of fluorescein dye. This means that light damage to photoreceptors by exciting light and problems induced by allergic reaction with fluorescein dye might be decreased. These aspects might be very important to obtain information about both retinal circulation and disruption of the blood-retinal barrier and also to perform safe fluorescein angiography examination.
Kasai
M
M
Department of Ophthalmology, Tohoku University School of Medicine, Sendai, Japan.
Suzuki
H
H
Tamai
M
M
Tasaki
K
K
D000818:Animals; D001813:Blood-Retinal Barrier; D019793:Fluorescein; D005451:Fluorescein Angiography / Q000379:methods*; D005452:Fluoresceins / Q000008:administration & dosage; D006801:Humans; D007089:Image Enhancement / Q000295:instrumentation; D008027:Light; D011892:Rana catesbeiana; D014741:Video Recording
D005452:Fluoresceins; D019793:Fluorescein