Endogenous digitalis-like factor as a stimulator of endothelin secretion from endothelial cells.
Effects of human urine-derived endogenous digitalis-like factor (EDLF) and ouabain on endothelin (ET) secretion were examined in cultured endothelial cells. ET was secreted in a linear fashion over 5 hours from bovine pulmonary artery endothelium into serum-free medium. EDLF stimulated ET secretion in a dose-dependent manner. In contrast, ouabain did not affect ET secretion at the concentration of 10(-9)-10(-5) M. These results indicate that human urine-derived EDLF is distinct from plant-derived ouabain and act as a stimulator of ET secretion by endothelial cells.
Yamada
K
K
Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Goto
A
A
Hui
C
C
Sugimoto
T
T
D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification / Q000494:pharmacology* / Q000652:urine; D002298:Cardenolides; D002417:Cattle; D002478:Cells, Cultured; D004077:Digoxin; D016232:Endothelins / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D010042:Ouabain / Q000494:pharmacology*; D011651:Pulmonary Artery; D011863:Radioimmunoassay; D012503:Saponins
D001798:Blood Proteins; D002298:Cardenolides; D016232:Endothelins; D012503:Saponins; C034504:digoxin-like factors; D010042:Ouabain; D004077:Digoxin