[Cloning and expression of a promoter function fragment from Thiobacillus thiooxidans in Escherichia coli].
This paper reports a recombinant plasmid pSDR12 which is constructed through the substitution of the EcoRI-HindIII fragment of pBR322 by a specific fragment of chromosomal DNA of T. thiooxidans. After it was transformed into C600, the transformants revealed higher levels of Tc resistance. This result shows that a promoter function fragment from autotrophic bacteria is able to express in Escherichia coil.
Yan
W
W
Institute of Microbiology, Shandong University, Jinan.
D013856:Acidithiobacillus thiooxidans / Q000235:genetics*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping