Fractionation and characterization of mitogenic and anti-complementary active fractions from kampo (Japanese Herbal) medicine "juzen-taiho-to".
"Juzen-Taiho-To" (TJ-48), which is a kampo (Japanese herbal) medicine prepared by decocting a prescription of ten kinds of herbs, has several immunostimulating activities. In order to characterize the active substances for anti-complementary and mitogenic activities, TJ-48 was fractionated. Anti-complementary activity was observed in the water- and methanol-insoluble fraction (F-2) and the crude polysaccharide fraction (F-5), whereas mitogenic activity was only found in F-5. However, other low molecular mass fractions did not show both activities. Methylation analysis indicated that F-2 mainly contained amylopectin-like polysaccharides. Both Pronase digestion and periodate oxidation decreased the anti-complementary activity of F-2, and the beta-amylase-resistant fraction of F-2 still retained the potent anti-complementary activity. When F-5, which has the most potent of both activities, was further fractionated, only the major acidic polysaccharide fraction, F-5-2, showed potent mitogenic activity. Endo-alpha-(1----4)-polygalacturonase digestion showed that F-5-2 mainly contained pectic polysaccharides, and the endo-polygalacturonase treatment of F-5-2 reduced the mitogenic activity but not the anti-complementary activity. F-2 and F-5 each activated the complement system by a different mode of action.
Yamada
H
H
Oriental Medicine Research Center of the Kitasato Institute, Tokyo, Japan.
Kiyohara
H
H
Cyong
J C
JC
Takemoto
N
N
Komatsu
Y
Y
Kawamura
H
H
Aburada
M
M
Hosoya
E
E
D000818:Animals; D002478:Cells, Cultured; D005591:Chemical Fractionation; D003169:Complement Inactivator Proteins / Q000302:isolation & purification*; D004247:DNA / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry*
D003169:Complement Inactivator Proteins; D004365:Drugs, Chinese Herbal; D008934:Mitogens; D004247:DNA