[Differences in the photodestruction parameters of flavin fluorescence in normal and tumor cells at a decreased pH of the incubation medium].
Further investigation of the peculiarities of flavin fluorescence photodestruction in malignant cells was made. In normal cells incubated in low pH (3.0-3.2) physiological solutions, the decrease in the oxidized flavoprotein fluorescence intensity under irradiation is the same as in normal pH condition, whereas in tumor cell in low pH solutions a significant increase in the photodestruction level was noticed. The cells isolated from foci of transformation, following treatment of 3T3 NIH fibroblasts with the carcinogen N-methyl-N'-nitro-N-nitrosoguanidine, displayed the photodestruction parameters similar to those in tumor cells. A two-step analysis of cells is proposed for distinguishing between the normal and malignant cells.
Sergeenko
N G
NG
Sal'nikov
K V
KV
Lebedev
O E
OE
D000818:Animals; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D002478:Cells, Cultured / Q000378:metabolism / Q000528:radiation effects; D003592:Cytophotometry; D005415:Flavins / Q000032:analysis / Q000528:radiation effects*; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D051379:Mice; D051381:Rats; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000528:radiation effects
D005415:Flavins; D008769:Methylnitronitrosoguanidine
Razlichia v parametrakh fotodestruktsii flavinovoĭ fluorestsentsii normal'nykh i opukholevykh kletok pri snizhenii pH sredy inkubatsii.