title: Presence of Philadelphia (Ph) chromosome-negative cells in Ph-positive chronic myelogenous leukemia.
abstract: Six cases of chronic myelogenous leukemia (CML) with coexistence of cells with a Philadelphia (Ph) translocation and a normal karyotype are described. Molecular analyses were performed in 3 of the 6 cases at two phases with different cytogenetic findings. Among the 6 cases, 4 cases were late appearance of a Ph chromosome. Two of the 3 patients analyzed had major breakpoint cluster region (Mbcr) rearrangements; aberrant Mbcr fragments were observed whether the cells had a Ph translocation or not. The remaining one did not show any rearrangements within the Mbcr DNA sequences.
authors.affiliation: First Department of Internal Medicine, Tokyo Medical College, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ohyashiki
authors.forename: J H
authors.initials: JH
authors.lastname: Ohyashiki
authors.forename: H
authors.initials: H
authors.lastname: Iwabuchi
authors.forename: T
authors.initials: T
authors.lastname: Tauchi
authors.forename: A
authors.initials: A
authors.lastname: Iwabuchi
authors.forename: K
authors.initials: K
authors.lastname: Toyama
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007621:Karyotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010677:Philadelphia Chromosome