title: Binding of viridans group streptococci to human platelets: a quantitative analysis.
abstract: The binding of viridans group streptococci with human platelets was analyzed by two-color flow cytometry. Binding was detected within 15 s of mixing bacteria and platelets. At ratios of bacteria to platelets of 1:1, 10:1, 100:1, and 1,000:1, the percentages of bound streptococci (mean +/- standard deviation) were 93.2% +/- 5.4%, 80.0% +/- 8.6%, 39.8% +/- 11.1%, and 12.5% +/- 2.0%, respectively. Binding of labeled bacteria was reversed by adding a 500-fold excess of unlabeled streptococci. These results demonstrate that streptococcus-platelet binding is rapid, reversible, and saturable, which suggests a specific receptor-ligand interaction.
authors.affiliation: Center for Immunochemistry, University of California, San Francisco.
authors.forename: P M
authors.initials: PM
authors.lastname: Sullam
authors.forename: D G
authors.initials: DG
authors.lastname: Payan
authors.forename: P F
authors.initials: PF
authors.lastname: Dazin
authors.forename: F H
authors.initials: FH
authors.lastname: Valone
mesh_terms: D001792:Blood Platelets / Q000382:microbiology* / Q000648:ultrastructure; D005434:Flow Cytometry; D006801:Humans; D013291:Streptococcus / Q000378:metabolism* / Q000648:ultrastructure; D013298:Streptococcus sanguis / Q000378:metabolism / Q000648:ultrastructure