Gene complementing xeroderma pigmentosum group A cells maps to distal human chromosome 9q.
Phenotypic complementation of xeroderma pigmentosum group A (XP-A) cells by microcell-mediated transfer of a single rearranged neo-tagged human chromosome from a human-mouse somatic cell hybrid designated K3SUB1A9-3 was reported previously. Extended growth of this human-mouse hybrid in culture led to deletion of the small arm of the human chromosome, with concomitant loss of complementing ability when introduced into XP-A cells by microcell-mediated chromosome transfer. Cytogenetic analysis of both hybrids suggests that the complementing locus is on chromosome 9q22.2-q34.3, and Southern blot analysis confirms the presence of distal chromosome 9q sequences.
Henning
K A
KA
Department of Genetics, Stanford University School of Medicine, California 94305.
Schultz
R A
RA
Sekhon
G S
GS
Friedberg
E C
EC
D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9; D003582:Cytogenetics; D005816:Genetic Complementation Test; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D014162:Transfection; D014178:Translocation, Genetic; D014983:Xeroderma Pigmentosum / Q000235:genetics*