Effects of reduced dietary magnesium on platelet production and function in hamsters.
The increased vulnerability of animals fed a magnesium (Mg)-deficient (MD) diet to ischemia-induced myocardial necrosis has been attributed to changes in myocardial electrolyte metabolism. However, a variety of hematologic changes have also been reported in MD and some of these, such as an increase in platelet aggregability, may contribute to the increased myocardial vulnerability. In the present study, we quantified the effect of MD on platelet and megakaryocyte abundance as well as on platelet aggregability with and without an administered calcium channel blocker (nifedipine). Hamsters were fed either a "minimal Mg" diet, in which the level of Mg was kept just high enough to prevent seizures, or a "preset Mg" diet containing precisely known amounts of Mg. Animals fed the minimum Mg diet showed an initial increase in the platelet count, which returned to control range when the dietary Mg was increased to 9 mmoles/kg. Animals on the preset Mg diet showed an increased platelet count if the Mg level was 10 mmoles/kg or less. In addition to the increase in number, platelets from MD animals were less responsive to the aggregation-inhibiting effect of nifedipine than were platelets from control animals, although MD itself did not result in an increased aggregability under the conditions used here. Animals with an increase in circulating platelets showed decreased megakaryocyte abundance in the femoral marrow, but megakaryocytes that were present were larger than those in control animals. These results indicate a profound effect of dietary Mg deficiency on platelet biology. The observed changes could contribute to the increase in myocardial vulnerability to injury found in MD animals.
Rishi
M
M
Department of Pathology, School of Medicine, George Washington University, Washington, D.C.
Ahmad
A
A
Makheja
A
A
Karcher
D
D
Bloom
S
S
D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D001835:Body Weight; D003327:Coronary Disease / Q000378:metabolism / Q000473:pathology; D006224:Cricetinae; D004032:Diet; D008275:Magnesium Deficiency / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D009543:Nifedipine / Q000494:pharmacology; D010974:Platelet Aggregation; D010976:Platelet Count
D009543:Nifedipine