[The demonstration of potential-dependent potassium channels in the plasma membrane of secretory cells].
Outward current of the salivary gland cells membrane of chironomus larva activated by the displacement of the membrane potential to the region of positive values has been registered by the voltage-clamp method under conditions of intracellular dialysis in the presence of only the potassium transmembrane gradient. Activation threshold of the current is about +10 mV. Subsequent displacement of the membrane potential to the region of positive values causes an increase of the current. Time constant of the current activation is (652 +/- 57) ms. The current decreases with the intracellular potassium concentration, under the influence of tetraethyl-ammonium and 4-aminopyridine. Thus, high threshold potential-dependent potassium channels are presented in the secretory cells membrane.
Klevets
M Iu
MIu
D015761:4-Aminopyridine / Q000494:pharmacology; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured / Q000187:drug effects / Q000502:physiology; D002683:Chironomidae; D007814:Larva; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D011188:Potassium / Q000502:physiology; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology*; D012469:Salivary Glands / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D013757:Tetraethylammonium Compounds / Q000494:pharmacology
D015221:Potassium Channels; D013757:Tetraethylammonium Compounds; D015761:4-Aminopyridine; D011188:Potassium
Vyiavlenie potentsialozavisimykh kalievykh kanalov plazmaticheskoĭ membrany sekretornykh kletok.