[Studies on cultured rat mesangial cells using cyclosporin A and magnesium--is magnesium nephroprotective in cyclosporin A therapy?].
One serious side effect of Cyclosporine A therapy is its acute nephrotoxicity characterized by a marked decrease in the glomerular filtration rate. A main determinant of the glomerular filtration rate is the ultrafiltration coefficient which is thought to be regulated by the contractile state of the cells of the mesangium. Cyclosporine A enhances contractions of mesangial cells elicited with angiotensin II. By way of lowered ultrafiltration coefficient this effect of Cyclosporine A may be partly responsible for its acute nephrotoxicity. Hypomagnesaemia is often associated with Cyclosporine A therapy. Profound tubular magnesium wasting by Cyclosporine A has been claimed its cause. We have investigated the effect of low and high magnesium concentration on the contractility of mesangial cells pretreated with Cyclosporine A. Without magnesium 80% of the cells contracted upon addition of angiotensin II. A marked decrease in the contractility was seen when the magnesium concentration was elevated to 2 mmol/l (34%). From these observations we conclude that magnesium serum levels even in the high normal range might be protective against the decrease of the glomerular filtration rate seen with CsA therapy.
Rob
P M
PM
Klinik für Innere Medizin, Medizinischen Universität zu Lübeck.
Fandrey
J
J
D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D003524:Cyclosporins / Q000633:toxicity*; D015232:Dinoprostone / Q000378:metabolism; D005919:Glomerular Filtration Rate / Q000187:drug effects; D005920:Glomerular Mesangium / Q000166:cytology / Q000187:drug effects*; D008274:Magnesium / Q000494:pharmacology*; D051381:Rats; D013268:Stimulation, Chemical
D003524:Cyclosporins; D000804:Angiotensin II; D008274:Magnesium; D015232:Dinoprostone
Untersuchungen an Rattenmesangialzellen in Kultur mit Cyclosporin A und Magnesium--Ist Magnesium nephroprotektiv bei Cyclosporin A-Therapie?