Characterization of cyanide-resistant respiration and appearance of a 36 kDa protein in mitochondria isolated from antimycin A-treated Hansenula anomala.
Mitochondria exhibiting cyanide-resistant respiration were isolated from Hansenula anomala which had been incubated in the presence of antimycin A to induce cyanide-resistant respiration. The cyanide-resistant respiration in isolated mitochondria was not inhibited by antimycin A or myxothiazol, suggesting that the branching of the pathway from the normal cyanide-sensitive pathway takes place at the coenzyme Q level. Analysis of mitochondrial proteins by sodium dodecyl sulfate gel electrophoresis indicated that a 36 kDa protein was induced by antimycin A treatment of the yeast. It is suggested that this protein is a component of the cyanide-resistant respiratory pathway.
Sakajo
S
S
Department of Biochemistry, Niigata College of Pharmacy.
Minagawa
N
N
Komiyama
T
T
Yoshimoto
A
A
D000935:Antifungal Agents / Q000494:pharmacology; D000968:Antimycin A / Q000494:pharmacology*; D002499:Centrifugation, Density Gradient; D003486:Cyanides / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis / Q000378:metabolism*; D008689:Methacrylates; D008715:Methionine / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010101:Oxygen Consumption / Q000187:drug effects; D010843:Pichia / Q000187:drug effects / Q000378:metabolism*; D013462:Sulfur Radioisotopes; D013844:Thiazoles / Q000494:pharmacology; D014451:Ubiquinone / Q000378:metabolism
D000935:Antifungal Agents; D003486:Cyanides; D005656:Fungal Proteins; D008689:Methacrylates; D013462:Sulfur Radioisotopes; D013844:Thiazoles; D014451:Ubiquinone; D000968:Antimycin A; C030517:myxothiazol; D008715:Methionine