title: Mapping of the functional domains of the v-rel oncogene.
abstract: Previously, the v-rel oncogene was shown to code for a protein of 503 amino acids. The protein product of v-rel was identified as a 59 kDa protein (pp59v-rel), phosphorylated predominantly on serine residues. Although the signal required for the nuclear localization of pp59v-rel in chicken embryo fibroblasts was identified, the regions of v-rel important for transformation have not been mapped. In this study, 12 linker insertion mutants of v-rel were constructed and tested for transforming activity. Seven linker insertion mutants which mapped between amino acid residues 29 and 275 abolished transformation. The remaining 5 mutants which contained linker insertion mutations between amino acid residues 332 and 459 transformed at wild type levels. The results of this analysis localize the functional domains of the v-rel oncogene to the N-terminus. Earlier reports have shown that pp59v-rel resides in a high molecular weight complex with several other cellular proteins. The transforming mutants co-precipitated the same set of cellular proteins when immunoprecipitated with v-rel antiserum. This indicates that all transforming mutants retained the ability to bind within the reported complex.
authors.affiliation: Department of Microbiology & Immunology, Faculty of Medicine, University of Ottawa, Ontario, Canada.
authors.forename: K
authors.initials: K
authors.lastname: Garson
authors.forename: C Y
authors.initials: CY
authors.lastname: Kang
mesh_terms: D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D020572:Oncogene Proteins v-rel; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics*
chemical_list: D020572:Oncogene Proteins v-rel; D015682:Retroviridae Proteins, Oncogenic