Inhibition of aromatase activity by 8-bromo-cyclic adenosine monophosphate in cultured first-trimester human trophoblast.
We have previously shown that 8-bromo-adenosine-3':5'-cyclic monophosphate inhibits production of estradiol from androgen precursors in trophoblast isolated from term placentas. In these experiments we sought to evaluate the effect of 8-bromo-adenosine-3':5'-cyclic monophosphate on aromatase activity in first-trimester trophoblast culture. Trophoblast cells were enzyme dispersed from first-trimester placentas and purified on a Percoll gradient. On the third and fourth day after dispersion cultures were treated with androstenedione or testosterone with and without 2 mmol/L 8-bromo-adenosine-3':5'-cyclic monophosphate. The quantity of estradiol, estrone, progesterone, human placental lactogen, and human chorionic gonadotropin was determined by radioimmunoassay. We concluded that in first-trimester trophoblast, as previously reported in term trophoblast, 8-bromo-adenosine-3':5'-cyclic monophosphate inhibited the production of estradiol from the supplied androgen precursors. Human chorionic gonadotropin and human placental lactogen production was stimulated by 8-bromo-adenosine-3':5'-cyclic monophosphate. Progesterone production was either stimulated or was unchanged by the presence of 8-bromo-adenosine-3':5'-cyclic monophosphate.
Rodway
M R
MR
Department of Obstestrics and Gynecology, University of British Columbia, Grace Hospital, Vancouver, Canada.
Yuen
B H
BH
Leung
P C
PC
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology*; D000735:Androstenedione / Q000378:metabolism; D047072:Aromatase Inhibitors; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000096:biosynthesis; D004958:Estradiol / Q000096:biosynthesis; D004970:Estrone / Q000096:biosynthesis; D005260:Female; D006801:Humans; D010928:Placental Lactogen / Q000096:biosynthesis; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011374:Progesterone / Q000096:biosynthesis; D013739:Testosterone / Q000378:metabolism; D014327:Trophoblasts / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism
D047072:Aromatase Inhibitors; D006063:Chorionic Gonadotropin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D004970:Estrone; D013739:Testosterone; D000735:Androstenedione; D011374:Progesterone; D004958:Estradiol; D010928:Placental Lactogen