title: Activated neutrophils induce erythrocyte immunoglobulin binding and membrane protein degradation.
abstract: Neutrophil-induced alterations in feline erythrocytes were investigated to elucidate the pathogenesis of the erythrocyte destruction associated with inflammatory disease. Incubation of erythrocytes with zymosan-activated neutrophils, at target:effector concentrations of 50:1, resulted in immunoglobulin binding to the erythrocyte membrane and degradation of membrane proteins. Vitamin E and superoxide dismutase/catalase attenuated the immunoglobulin binding. Proteolysis was not prevented by addition of antioxidants or protease inhibitors to the erythrocyte:neutrophil coculture and was not reproduced with chemical oxidation or calcium loading of erythrocytes.
authors.affiliation: Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
authors.forename: D J
authors.initials: DJ
authors.lastname: Weiss
authors.forename: M P
authors.initials: MP
authors.lastname: Murtaugh
mesh_terms: D000818:Animals; D002415:Cats; D010450:Endopeptidases / Q000502:physiology; D004910:Erythrocyte Membrane / Q000378:metabolism*; D007074:Immunoglobulin G / Q000378:metabolism*; D007249:Inflammation / Q000097:blood; D008565:Membrane Proteins / Q000378:metabolism*; D009504:Neutrophils / Q000502:physiology*
chemical_list: D007074:Immunoglobulin G; D008565:Membrane Proteins; D010450:Endopeptidases