[The rapid diagnosis of measles by using immunoenzyme analysis based on peroxidase-labelled viral antigens].
The sensitivity and specificity of the enzyme immunoassay (the capture variant), based on the use of the complex of anti-IgM-IgM to purified measles virus, the peroxidase-labeled lysate and nucleocapsid antigens of measles virus, were evaluated. The advantages of the nucleocapsid conjugate were established. The study of 200 serum samples from measles patients revealed that antimeasles IgM antibodies could be detected in 100% of cases, and the assay of 15 serum samples from healthy donors and 5 serum samples from patients with autoimmune diseases did not yield a single false positive result.
Vedunova
S L
SL
Mal'tseva
N N
NN
D000974:Antibodies, Antinuclear / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral; D001327:Autoimmune Diseases / Q000276:immunology; D005189:False Positive Reactions; D006801:Humans; D007124:Immunoenzyme Techniques; D007075:Immunoglobulin M / Q000032:analysis; D008457:Measles / Q000175:diagnosis*; D008459:Measles virus / Q000276:immunology*; D012217:Rheumatoid Factor / Q000097:blood
D000974:Antibodies, Antinuclear; D000914:Antibodies, Viral; D000956:Antigens, Viral; D007075:Immunoglobulin M; D012217:Rheumatoid Factor
Ekspress-diagnostika kori s pomoshch'iu immunofermentnogo analiza na osnove mechennykh peroksidazoĭ virusnykh antigenov.