title: Release of T-antigen, a carcinoma marker from native human cells, by endo-alpha-N-acetylgalactosaminidase of Alcaligenes sp.
abstract: The endo-alpha-N-acetylgalactosaminidase from Alcaligenes sp. could release T-antigen (Ga1 beta 1----3GalNAc), a carcinoma-associated marker from asialo glycophorin and human cells. The released T-antigen from human asialo erythrocytes was determined by thin layer chromatography and gas liquid chromatography. The released T-antigen from human gastric carcinoma cell Kato III was identified by high performance liquid chromatographies with a reverse-phase column and a size fractionation column. Released T-antigen could be analyzed quantitatively by a sensitive method including pyridylamino derivatization and following high performance liquid chromatography. This suggests that the enzyme is useful for detection and determination of T-antigen from cells.
authors.affiliation: Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan.
authors.forename: J Q
authors.initials: JQ
authors.lastname: Fan
authors.forename: K
authors.initials: K
authors.lastname: Kanatani
authors.forename: N
authors.initials: N
authors.lastname: Andoh
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.forename: H
authors.initials: H
authors.lastname: Kumagai
authors.forename: T
authors.initials: T
authors.lastname: Tochikura
mesh_terms: D000421:Alcaligenes / Q000201:enzymology*; D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor / Q000032:analysis*; D002460:Cell Line; D002846:Chromatography, Affinity; D004187:Disaccharides / Q000032:analysis*; D004912:Erythrocytes / Q000276:immunology; D006021:Glycophorins / Q000302:isolation & purification; D006596:Hexosaminidases / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D013274:Stomach Neoplasms; D048809:alpha-N-Acetylgalactosaminidase
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor; D004187:Disaccharides; D006021:Glycophorins; C016467:Thomsen-Friedenreich antigen; D006596:Hexosaminidases; C482722:NAGA protein, human; D048809:alpha-N-Acetylgalactosaminidase