Erythrocyte antibody screening in solid-phase: a comparison of two solid-phase microplate assays with the indirect antiglobulin test in polyethylene glycol for the detection of irregular erythrocyte antibodies.
The screening of a serum for irregular erythrocyte antibodies in the indirect antiglobulin test is a well-established technique. We compared the test results of two different solid-phase microplate indirect antiglobulin tests with a liquid-phase indirect antiglobulin test in tubes. Antibody screening with both solid-phase microplate techniques proved to be more sensitive than the liquid-phase indirect antiglobulin test. In addition, a difference in sensitivity between the two solid-phase techniques was observed: prior immobilization of test erythrocytes on the microplate followed by incubation with a serum and detection of sensitization with antihuman IgG-coated detector cells gave better test results than secondary immobilization on the microplate of test erythrocytes sensitized with antibodies and an antihuman globulin serum.
Hazenberg
C A
CA
Blood Grouping Laboratory, University Hospital, Groningen, The Netherlands.
Mulder
M B
MB
Beelen
J M
JM
D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity / Q000276:immunology; D001323:Autoantibodies / Q000032:analysis; D003298:Coombs Test / Q000295:instrumentation; D004912:Erythrocytes / Q000276:immunology*; D006384:Hemagglutination / Q000276:immunology; D006801:Humans; D007518:Isoantibodies / Q000032:analysis; D008722:Methods; D011092:Polyethylene Glycols
D000906:Antibodies; D001323:Autoantibodies; D007518:Isoantibodies; D011092:Polyethylene Glycols