[The cultivation of hybridoma cells in agarose granules].
The results of cultivation of agarose gel immobilized hybridoma cells producing monoclonal antibodies to human alpha 2-interferon are presented. The immobilized cultured cells retained the higher viability for 10-28 days without changing the growth medium, in comparison with cultured cells in control suspension. The cells immobilized in agarose gel demonstrated a tendency to polyploidization as was revealed by the technique of DNA cytofluorimetry with Hoechst 33258.
Kukhareva
L V
LV
D'iakonov
I A
IA
Blinova
M I
MI
Nikolaenko
N S
NS
Pinaev
G P
GP
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D002470:Cell Survival; D003470:Culture Media; D003584:Cytological Techniques; D004247:DNA / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D006825:Hybridomas / Q000737:chemistry / Q000166:cytology* / Q000276:immunology; D007370:Interferon Type I / Q000276:immunology; D051379:Mice; D011123:Polyploidy; D012685:Sepharose; D013997:Time Factors
D000911:Antibodies, Monoclonal; D003470:Culture Media; D007370:Interferon Type I; D004247:DNA; D012685:Sepharose
Kul'tivirovanie kletok gibridomy v granulakh iz agarozy.