[Three-dimensional culture of plucked human hair follicles inside the collagen gel matrix].
Plucked human hair follicles were cultured in collagen gel matrix. Epithelial cells, possibly outer root sheath keratinocytes, appeared from the outer root sheath 4 to 5 days after culturing and continually grew into the gel to form spike-like structures for next 3 weeks. The number and size of the spikes differed in each follicle. Autoradiographically, many DNA-synthesizing cells were seen at the outer cell layer in the enlarged outer root sheath and at the edges in the newly formed spike-like structures. The culture method described here appears to be suitable to study the three-dimensional growth, morphogenesis and differentiation of the outer root sheath cells in vitro.
Arase
S
S
Department of Dermatology, School of Medicine, University of Tokushima.
Sadamoto
Y
Y
Kato
S
S
Fujie
K
K
Nakanishi
H
H
Takeda
K
K
D000328:Adult; D002478:Cells, Cultured; D003094:Collagen; D003470:Culture Media; D003584:Cytological Techniques; D005260:Female; D005782:Gels; D006197:Hair / Q000166:cytology*; D006801:Humans; D008297:Male
D003470:Culture Media; D005782:Gels; D003094:Collagen