title: [A new method of purification of L-lysine-alpha-oxidase from the fungus Trichoderma].
abstract: A new procedure is developed for purification of the antitumoral enzyme L-lysyl-alpha-oxidase from Trichoderma sp. The procedure included two steps: hydrophobic chromatography on butyl sylochrome C-80 and chromatography using biospecific sorbent AH-Sepharose. The simplified procedure enabled to increase distinctly the specific enzymatic activity from 30 to 50 IU/mg in the preparation obtained with a good yield.
authors.forename: N F
authors.initials: NF
authors.lastname: Laugalene
authors.forename: V S
authors.initials: VS
authors.lastname: Vesa
authors.forename: R P
authors.initials: RP
authors.lastname: Iankiavichene
authors.forename: S P
authors.initials: SP
authors.lastname: Puodzhiute
authors.forename: O F
authors.initials: OF
authors.lastname: Sudzhiuvene
authors.forename: I I
authors.initials: II
authors.lastname: Pesliakas
authors.forename: S Kh
authors.initials: SKh
authors.lastname: Khaduev
authors.forename: T T
authors.initials: TT
authors.lastname: Berezov
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000302:isolation & purification*; D002845:Chromatography / Q000379:methods; D014242:Trichoderma / Q000201:enzymology*
chemical_list: D000594:Amino Acid Oxidoreductases; C041123:L-lysine oxidase
vernacular_title: Novyĭ metod ochistki L-lizin-alpha-oksidazy griba Trichoderma.