title: Evaluation of a selective medium for the isolation and differentiation of Haemophilus influenzae and Haemophilus parainfluenzae from the respiratory tract of chronic bronchitics.
abstract: Respiratory tract specimens from chronic bronchitic patients were cultured for Haemophilus species on conventional chocolate agar and a modified sucrose medium in order to determine the accuracy of the new medium in differentiating Haemophilus influenzae from Haemophilus parainfluenzae strains. Haemophilus influenzae biotypes II and III and Haemophilus parainfluenzae biotypes I and II were found to be the predominant strains isolated from the respiratory tract. The modified sucrose medium was found to be a rapid and reliable means of differentiating Haemophilus influenzae from Haemophilus parainfluenzae by sucrose fermentation, on initial isolation.
authors.affiliation: Faculty of Medicine, University of Newcastle, NSW.
authors.forename: D C
authors.initials: DC
authors.lastname: Taylor
authors.forename: A W
authors.initials: AW
authors.lastname: Cripps
authors.forename: R L
authors.initials: RL
authors.lastname: Clancy
authors.forename: K
authors.initials: K
authors.lastname: Murree-Allen
authors.forename: M J
authors.initials: MJ
authors.lastname: Hensley
authors.forename: N A
authors.initials: NA
authors.lastname: Saunders
authors.forename: D C
authors.initials: DC
authors.lastname: Sutherland
mesh_terms: D001991:Bronchitis / Q000175:diagnosis / Q000382:microbiology*; D002908:Chronic Disease; D003470:Culture Media; D003937:Diagnosis, Differential; D006190:Haemophilus / Q000302:isolation & purification*; D006192:Haemophilus Infections / Q000175:diagnosis*; D006193:Haemophilus influenzae / Q000302:isolation & purification*; D006801:Humans; D012137:Respiratory System / Q000382:microbiology*; D013045:Species Specificity
chemical_list: D003470:Culture Media