Rat lactate dehydrogenase A and B subunit concentrations are not regulated by mRNA abundance in liver and heart.
RNA was isolated from rat liver and heart tissues at various times up to 12 weeks after birth, and probed on slot blots with lactate dehydrogenase A and B cDNA probes. Although the relative abundances of LDH A in liver and LDH B in heart increased substantially in the 12 weeks after birth, mRNAs for both isoenzymes remained remarkably stable in both tissues over the same period. The implications of these observations for the regulation of constitutive gene expression are discussed.
Skidmore
A F
AF
Department of Biochemistry, University of Sussex, Falmer, Brighton, UK.
Beebee
T J
TJ
D000375:Aging / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn / Q000235:genetics; D015152:Blotting, Northern; D015342:DNA Probes; D015971:Gene Expression Regulation, Enzymologic; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D009206:Myocardium / Q000201:enzymology*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D015342:DNA Probes; D046911:Macromolecular Substances; D012333:RNA, Messenger; D007770:L-Lactate Dehydrogenase