DNA probes for the identification of Nocardia asteroides.
DNA probes for the rapid identification of Nocardia asteroides were obtained by constructing a genomic library of strain GUH-2 in the lambda cloning vector EMBL3. Of 50 recombinant clones tested, 2 were identified that hybridized with 31% of the N. asteroides strains in a reference collection without cross-hybridization with related members of the Actinomycetales. Additional libraries were then generated from selected strains of N. asteroides that had failed to hybridize with any of the GUH-2 clones. Four additional clones were obtained from these strains which, when pooled, provided DNA probes specific for all of the N. asteroides strains tested.
Brownell
G H
GH
Department of Cell and Molecular Biology, Medical College of Georgia, Augusta 30912.
Belcher
K E
KE
D015342:DNA Probes; D015723:Gene Library; D006801:Humans; D009617:Nocardia Infections / Q000175:diagnosis; D009616:Nocardia asteroides / Q000235:genetics* / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D013045:Species Specificity
D015342:DNA Probes