title: The amino-terminal 29- and 72-Kd fragments of fibronectin mediate selective monocyte recruitment.
abstract: Proteolytic fragments of fibronectin (Fn) can possess properties not inherent to intact Fn. Previously, only mixtures of low molecular weight Fn fragments, and the 120-Kd fibroblastic cell-binding segment, but not intact Fn, were shown to be selectively chemotactic for human monocytes (MOs). In order to determine if other structural domains of Fn were responsible, we tested six Fn fragments. The amino-terminal 72-Kd fragment at 1.5 microns was about 75% as potent as zymosan-activated serum (ZAS). Its amino-terminal 29-Kd degradation product at 1.0 micron was about one third as potent as ZAS. Checkerboard analysis confirmed chemotaxis. Complexing gelatin to 72-Kd fragments reduced MO chemotaxis by 28% to 30%. Reducing disulfide bonds in 29- and 72-Kd segments had no effect. A synthetic peptide containing the thrombin cleavage site between the 29- and 50-Kd segments of the 72-Kd fragment was chemotactic. The 50-, 190/170-, 35-, and 160/150/120-Kd fragments, and intact Fn were not chemotactic for MOs. The data suggest that the 72-Kd fragment and its 29-Kd subfragment are additional Fn fragments that mediate selective MO chemotaxis. We speculate that proteinases present at inflammatory sites can liberate such fragments that selectively recruit MOs.
authors.affiliation: Department of Medicine, University of Tennessee, Memphis.
authors.forename: K M
authors.initials: KM
authors.lastname: Lohr
authors.forename: C A
authors.initials: CA
authors.lastname: Kurth
authors.forename: D L
authors.initials: DL
authors.lastname: Xie
authors.forename: J M
authors.initials: JM
authors.lastname: Seyer
authors.forename: G A
authors.initials: GA
authors.lastname: Homandberg
mesh_terms: D002633:Chemotaxis / Q000187:drug effects / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D005353:Fibronectins / Q000032:analysis / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D009000:Monocytes / Q000187:drug effects* / Q000502:physiology; D010446:Peptide Fragments / Q000032:analysis / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D005353:Fibronectins; D010446:Peptide Fragments