title: Hinf I restriction endonuclease digestion on human fixed metaphase chromosomes.
abstract: The authors report on the activity of Hinf I restriction endonuclease on human fixed metaphase chromosomes. Experiments performed by digesting chromosomes just after harvesting or after ageing in methanol-acetic acid displayed a different pattern of digestion on metaphases, since only aged preparations showed gaps on heterochromatic regions of chromosomes 1, 9 and 16 and C-like bands on other chromosomes. In this view, the authors suggest that structural modifications of the DNA, induced by acid fixation, can influence Hinf I activity on fixed metaphase chromosomes.
authors.affiliation: Istituto di Citomorfologia, CNR, Chieti, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Stuppia
authors.forename: P
authors.initials: P
authors.lastname: Guanciali Franchi
authors.forename: G
authors.initials: G
authors.lastname: Calabrese
authors.forename: G
authors.initials: G
authors.lastname: Parruti
authors.forename: G
authors.initials: G
authors.lastname: Palka
authors.forename: U
authors.initials: U
authors.lastname: Bianchi
mesh_terms: D002875:Chromosomes / Q000737:chemistry / Q000187:drug effects*; D004247:DNA / Q000032:analysis / Q000187:drug effects; D015252:Deoxyribonucleases, Type II Site-Specific / Q000494:pharmacology*; D006801:Humans; D008677:Metaphase
chemical_list: D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases