Adherence of human neutrophils changes Ca2+ signaling during activation with opsonized particles.
Changes in the cytosolic free Ca2+ concentration ([Ca2+]i) upon activation of human neutrophils by opsonized particles (serum-treated zymosan; STZ) were evaluated by three different methods: (i) measurement of total fluorescence changes in indo-1 loaded neutrophils activated in suspension; (ii) measurement of fluorescence changes in individual indo-1 loaded neutrophils in a flow cytometer and (iii) measurement of fluorescence changes in individual fura-2 loaded neutrophils adherent to serum-coated coverslips. Our study shows that the opsonized particle-induced change in [Ca2+]i in neutrophils is altered during adherence of the cells to a serum-coated surface. These observations might be of importance for neutrophil function in vivo, since adherence is a prerequisite for diapedesis and chemotaxis.
Koenderman
L
L
Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, University of Amsterdam, The Netherlands.
Tool
A T
AT
Hooybrink
B
B
Roos
D
D
Hansen
C A
CA
Williamson
J R
JR
Verhoeven
A J
AJ
D002118:Calcium / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D009504:Neutrophils / Q000502:physiology*; D009895:Opsonin Proteins; D010587:Phagocytosis / Q000502:physiology*; D015398:Signal Transduction
D009895:Opsonin Proteins; D002118:Calcium