[The expression of an antigen interacting with monoclonal antibody IB4 on the surface membranes of normal human leukocytes and in lymphoproliferative diseases].
Monoclonal antibody (MAb) 1B4 of IgG3 isotype reacting with 55 +/- 10% peripheral blood lymphocytes was obtained. MAb 1B4 is bound with 100% of B and NK cells and 50-70% of T cells in blood. The most part of CD8+ cells (60-100%) and approximately 50% of CD4+ cells belong to 1B4+ population. T-ALL cells do not express 1B4 antigen. Analysis of reactivity of MAb 1B4 with transformed malignant cells has shown that 1B4 antigen appeared in the process of differentiation of B lymphocytes at the state of pre-B cells and disappeared with activation of B lymphocytes and transition to plasma cells.
Filatov
A V
AV
Bachurin
P S
PS
Sidorenko
S P
SP
Abramenko
I V
IV
Gluzman
D F
DF
D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D015703:Antigens, CD / Q000032:analysis / Q000276:immunology*; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D002471:Cell Transformation, Neoplastic / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007132:Immunoglobulin Isotypes / Q000276:immunology*; D015479:Leukemia, Myelomonocytic, Acute / Q000276:immunology; D007962:Leukocytes / Q000276:immunology*; D008232:Lymphoproliferative Disorders / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface; D007132:Immunoglobulin Isotypes
Izuchenie ékspressii antigena, vzaimodeĭstvuiushchego s monoklonal'nym antitelom IB4, na poverkhnostnykh membranakh leĭkotsitov cheloveka v norme i pri limfoproliferativnykh zabolevaniiakh.