title: Identification of phosphopeptides by mass spectrometry.
abstract: Phosphopeptides can be identified by ion spray mass spectrometry. The method was tested with phosphokemptide and with a proteolytic digest of one subunit (delta-subunit) of the nicotinic acetylcholine receptor. In the latter one peptide containing tyrosine phosphate, one with two serine phosphates, and two different peptides each containing one serine phosphate were unambiguously identified. Thus it is proven that ion spray mass spectrometry can be applied for the localization of phosphorylation sites in a known primary structure.
authors.affiliation: Institut für Biochemie, Freie Universität Berlin, FRG.
authors.forename: W
authors.initials: W
authors.lastname: Schroeder
authors.forename: T
authors.initials: T
authors.lastname: Covey
authors.forename: F
authors.initials: F
authors.lastname: Hucho
mesh_terms: D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry / Q000379:methods; D009842:Oligopeptides / Q000737:chemistry; D010748:Phosphopeptides / Q000032:analysis* / Q000138:chemical synthesis; D011506:Proteins / Q000737:chemistry; D011978:Receptors, Nicotinic / Q000737:chemistry
chemical_list: D009842:Oligopeptides; D010748:Phosphopeptides; D011506:Proteins; D011978:Receptors, Nicotinic; C037642:kemptide