Extraction method for preparing pyridylamino sugar derivatives and application to porcine gastric mucus glycoprotein analysis.
For the sensitive detection of free sugars and oligosaccharides, the use of their pyridylamino derivatives has now found general acceptance. To remove excess 2-aminopyridine from this derivative in a reaction mixture, gel filtration and ion-exchange chromatography were conducted. It was found in the present study that contaminated 2-aminopyridine could be selectively removed from the reaction mixture by adjusting the pH with saturated sodium bicarbonate at above 8.5 followed by extraction with benzene. By using this method, fewer purification steps and less time are required, with minimum loss of pyridylamino sugar derivatives.
Iwase
H
H
Department of Biochemistry, School of Medicine, Kitasato University, Kanagawa, Japan.
Ishii-Karakasa
I
I
Urata
T
T
Saito
T
T
Hotta
K
K
D000606:Amino Sugars / Q000138:chemical synthesis / Q000302:isolation & purification*; D000631:Aminopyridines / Q000302:isolation & purification*; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006023:Glycoproteins / Q000032:analysis*; D007202:Indicators and Reagents; D009093:Mucus; D013552:Swine
D000606:Amino Sugars; D000631:Aminopyridines; D002241:Carbohydrates; D006023:Glycoproteins; D007202:Indicators and Reagents; C032439:alpha-aminopyridine