Characterization of a gene encoding a manganese peroxidase from Phanerochaete chrysosporium.
The complete nucleotide (nt) sequence of a gene (mnp-1) encoding manganese peroxidase isozyme 1 (MnP-1) (pI = 4.9) from Phanerochaete chrysosporium has been determined. The sequence of 2539 bp includes 526 bp of 5'-flanking sequence and 368 bp 3' to the poly(A) site. Comparison of cDNA and genomic sequences indicates six introns varying in size from 57-72 bp. Intron splice-junction sequences all adhere to the GT---AG rule. The positions of the introns show little similarity to the intron positions in the closely related lignin peroxidase-encoding genes. The 5' upstream region of the mnp-1 gene contains a TATAA element and three inverted CCAAT elements (ATTGG) at nt positions -81, -181, -195, and -304, respectively, relative to the start codon. In addition, the mnp-1 gene contains three putative heat-shock (HS) elements similar to the consensus C--GAA--TTC--G sequence, and two consensus metal response elements located within 500 bp upstream from the start codon. Furthermore, Northern-blot analysis demonstrates that mnp gene transcription is regulated by HS.
Godfrey
B J
BJ
Department of Chemical and Biological Sciences, Oregon Graduate Institute of Science and Technology, Beaverton 97006-1999.
Mayfield
M B
MB
Brown
J A
JA
Gold
M H
MH
D000595:Amino Acid Sequence; D001483:Base Sequence; D001487:Basidiomycota / Q000235:genetics*; D015152:Blotting, Northern; D003001:Cloning, Molecular; D005800:Genes, Fungal; D007438:Introns; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000235:genetics*; D012331:RNA, Fungal / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping
D012331:RNA, Fungal; D012333:RNA, Messenger; D010544:Peroxidases; C051129:manganese peroxidase