[Effect of human interleukin-1 on cartilage metabolism].
This study was undertaken to elucidate the effect of human interleukin-1 (IL-1) on the cartilage metabolism, with H235SO4 and 3H-proline as the respective indices for the metabolism of proteoglycan and collagen. We examined in vitro the 35S and 3H rates of incorporation after IL-1 was added to free chondrocytes incubated from human and porcine joint cartilage. As a result, IL-1 suppressed dose-dependently the secretion of both 35S and 3H into the supernatant of the medium containing free human or porcine chondrocytes. There was no difference between the control and the IL-1 group in the elution pattern of proteoglycan on the column-chromatogram. These results indicated that human IL-1 did not affect the molecular weight of the proteoglycan produced by human and porcine chondrocytes.
Hondo
H
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Department of Orthopaedic Surgery, Nihon University School of Medicine, Tokyo, Japan.
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D003094:Collagen / Q000096:biosynthesis*; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology*; D011509:Proteoglycans / Q000096:biosynthesis*; D013552:Swine
D007375:Interleukin-1; D011509:Proteoglycans; D003094:Collagen