Detection of cathepsin B, plasminogen activators and plasminogen activator inhibitor in human non-small lung cancer cell lines.
Human non-small lung cancer cell lines HS-24 (established from a primary squamous cell carcinoma) and SB-3 (established from a metastasis of a primary adenocarcinoma of the lung into the adrenal gland) were analysed for the proteinases tissue-type plasminogen activator (tPA), urokinase-type plasminogen activator inhibitor (PAI-1). The proteinases were characterized by activity measurements, inhibition studies, enzyme-linked immunosorbent assay (ELISA), and Western blot analysis. Cell-associated proteinases were determined in cell lysates, secreted proteinases in cell conditioned culture media. Both cell lines were found to secrete uPA and PAI-1, whereas tPA could be detected only in HS-24 conditioned media. No cathepsin B activity could be detected in media of both cell lines. However, activation experiments and western blot analysis showed, that at least HS-24 secrete an inactive precursor. Cell lysates of HS-24 and SB-3 show PA activity, but on a low level. Cathepsin B activity was also found to be low in HS-24 lysates. However, SB-3 lysates show high cathepsin B activity. Further characterization of the proteinases by their sensitivity against several inhibitors suggests that they are similar to the corresponding proteinases of normal, nonmalignant cells.
Trefz
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Thoraxklinik Heidelberg-Rohrbach, Klinische Chemie und Bakteriologie.
Erdel
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M
Spiess
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Ebert
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W
D000230:Adenocarcinoma / Q000737:chemistry* / Q000556:secondary; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000556:secondary; D002401:Cathepsin B / Q000032:analysis* / Q000276:immunology; D006801:Humans; D008175:Lung Neoplasms / Q000737:chemistry* / Q000556:secondary; D010960:Plasminogen Activators / Q000032:analysis* / Q000276:immunology; D015849:Plasminogen Inactivators / Q000032:analysis* / Q000276:immunology; D014407:Tumor Cells, Cultured
D015849:Plasminogen Inactivators; D010960:Plasminogen Activators; D002401:Cathepsin B