[Identification of Campylobacter jejuni by using a biotinylated probe].
A DNA biotinylated probe for the rapid identification of Campylobacter jejuni in culture (Enzo Biochem, New York) has been evaluated. The hybridized biotinylated DNA probe is detected by its interaction with streptavidin linked to horseradish peroxidase. Sixteen strains of C. jejuni, including type strain. 24 strains of other Campylobacter and Helicobacter species, and 59 strains of other general have been studied. The probe was highly sensitive (100%) and specific (100% inside the genera Campylobacter and Helicobacter). All Campylobacter strains gave strong signals, and only three weak signals have been observed with non-Campylobacter strains. Our results indicate that specific recombinant DNA probe should offer a reliable and rapid method for routine diagnosis of Campylobacter jejuni enteritis.
Bollet
C
C
Laboratoire de Microbiologie et d'Hygiène Hospitalière, Hôpital Salvator, Marseille, France.
Bouchemal
H
H
Deltin
P
P
de Micco
P
P
D002168:Campylobacter fetus / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial; D006801:Humans; D066298:In Vitro Techniques; D015336:Molecular Probe Techniques; D009693:Nucleic Acid Hybridization
D004269:DNA, Bacterial
Identification de Campylobacter jejuni par une sonde biotinylée.