Acyl carrier protein interacts with melittin.
Acyl carrier protein (ACP) from Escherichia coli has been shown to form complexes with melittin, a cationic peptide from bee venom. ACP is a small (Mr 8847), acidic, Ca2(+)-binding protein, which possesses some characteristics resembling those of regulatory Ca2(+)-binding proteins including interaction with melittin. Complexing between melittin and ACP which occurred both in the presence and absence of Ca2+ was evident by chemical cross-linking the two peptides, fluorescence changes (including anisotropy measurements), and inhibition by melittin of the activity of a nonaggregated fatty acid synthetase from Euglena. Also, anti-Apis mellifera antibodies which contained antibodies against melittin specifically inhibited the same enzyme system activity relative to non-immune IgG.
Ernst-Fonberg
M L
ML
Department of Biochemistry, James H. Quillen College of Medicine, East Tennessee State University, Johnson City 37614-0002.
Williams
S G
SG
Worsham
L M
LM
D000213:Acyl Carrier Protein / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001514:Bee Venoms / Q000276:immunology; D015153:Blotting, Western; D002118:Calcium / Q000494:pharmacology; D003432:Cross-Linking Reagents; D004120:Dimethyl Suberimidate; D004926:Escherichia coli / Q000032:analysis*; D005055:Euglena / Q000201:enzymology; D064429:Fatty Acid Synthases / Q000494:pharmacology; D008555:Melitten / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D008970:Molecular Weight; D013050:Spectrometry, Fluorescence
D000213:Acyl Carrier Protein; D000906:Antibodies; D001514:Bee Venoms; D003432:Cross-Linking Reagents; D008555:Melitten; D004120:Dimethyl Suberimidate; D064429:Fatty Acid Synthases; D002118:Calcium