title: Interchangeable RNA polymerase I and II enhancers.
abstract: The RNA polymerase I (pol I) enhancer of Saccharomyces cerevisiae contains at least three elements commonly associated with RNA polymerase II (pol II) enhancers, binding sites for the transcriptional activators general regulatory factor 2 and autonomously replicating sequence-binding factor I, and a thymidine-rich element. When the particular form of the thymidine-rich element found in the pol I enhancer was placed in front of a pol II promoter, transcription was stimulated 43-fold, comparable to the effect of a powerful pol II activator such as Gal4. Conversely, when two copies of a thymidine-rich element from a pol II enhancer were placed upstream of a pol I promoter, transcription was stimulated 38-fold. This functional reciprocity of pol I and II enhancers may reflect similarities in the mechanisms of transcriptional activation. The pol I enhancer also contains an element that appears to be pol I-specific and prevent the activation of pol II.
authors.affiliation: Department of Cell Biology, Stanford University School of Medicine, CA 94305.
authors.forename: Y
authors.initials: Y
authors.lastname: Lorch
authors.forename: N F
authors.initials: NF
authors.lastname: Lue
authors.forename: R D
authors.initials: RD
authors.lastname: Kornberg
mesh_terms: D001483:Base Sequence; D004742:Enhancer Elements, Genetic; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010957:Plasmids; D012318:RNA Polymerase I / Q000235:genetics*; D012319:RNA Polymerase II / Q000235:genetics*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*
chemical_list: D015345:Oligonucleotide Probes; D012319:RNA Polymerase II; D012318:RNA Polymerase I