Membrane fatty acid changes during the cell cycle of CV-1 cells.
Monolayers of CV-1 cells were synchronized at the G1/S boundary of the cell cycle by a 24-h 2 mM thymidine blockade. Uptake of tritiated thymidine indicated that the peak DNA synthesis occurred 6-8 h after release from the block and that cell cycle time was 18-20 h. The fatty acid composition of phospholipids extracted from cells at 0, 7, and 18 h postblockade was measured by gas chromatography. The results indicate cyclic changes in membrane fatty acids with a significant increase in long-chain polyunsaturated fatty acids during the DNA synthesis phase (S phase) of the cell cycle.
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Department of Biology, University of Wollongong, New South Wales, Australia.
Sullivan-Tailyour
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Hulbert
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AJ
D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D002850:Chromatography, Gel; D004247:DNA / Q000096:biosynthesis; D005227:Fatty Acids / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism
D005227:Fatty Acids; D008563:Membrane Lipids; D010743:Phospholipids; D004247:DNA