Effects of chlorine dioxide on the developing rat brain.
Male and female Long-Evans rat pups, exposed to an oral dose of 14 mg chlorine dioxide (CIO2)/kg/d (postnatal d 1-20), were examined for effects on brain development and for changes in thyroid activity. Body weight reductions were observed on postnatal (pn) d 11, 21, and 35. Forebrain weight and protein content were decreased on pnd 21 and 35, as were the DNA content on d 35 and the number of dendritic spines on cerebral cortical pyramidal cells, a marker for synapse formation. Otherwise, cell proliferation in the forebrain, cerebellum, and olfactory bulbs was normal, as were migration and aggregation of neuronal cells in three areas of the cerebral cortex. Histopathology of the forebrain, cerebellum, and brainstem showed no gross lesions, loss of myelin, or change in the cells staining positive for Nissl substance. Serum T3 and T4 levels, as well as hepatic mitochondrial alpha-glycerophosphate dehydrogenase activity, were unchanged by CIO2 treatment. The results indicated that CIO2 may have central neurotoxic potential. No underlying antithyroid activity was evident.
Toth
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Reproductive and Developmental Biochemistry Branch, U.S. Environmental Protection Agency, Cincinnati, Ohio 45268.
Long
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RE
Mills
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Smith
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MK
D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000473:pathology; D001933:Brain Stem / Q000187:drug effects / Q000254:growth & development / Q000473:pathology; D002531:Cerebellum / Q000187:drug effects / Q000254:growth & development / Q000473:pathology; D002540:Cerebral Cortex / Q000187:drug effects* / Q000254:growth & development / Q000473:pathology; D002713:Chlorine / Q000633:toxicity*; D017606:Chlorine Compounds; D004247:DNA / Q000378:metabolism; D004202:Disinfectants / Q000633:toxicity*; D005260:Female; D008297:Male; D009830:Olfactory Bulb / Q000187:drug effects / Q000254:growth & development / Q000473:pathology; D009929:Organ Size / Q000187:drug effects; D010087:Oxides / Q000633:toxicity*; D011441:Propylthiouracil / Q000633:toxicity; D011506:Proteins / Q000378:metabolism; D051381:Rats; D013963:Thyroid Hormones / Q000378:metabolism
D017606:Chlorine Compounds; D004202:Disinfectants; D010087:Oxides; D011506:Proteins; D013963:Thyroid Hormones; D002713:Chlorine; D011441:Propylthiouracil; C025109:chlorine dioxide; D004247:DNA