A rapid method for monitoring DNA labelling reactions with haptens.
A rapid and simple method for evaluating the efficiency of DNA labelling reactions with haptens is described. The method, called the Flow-Through Hapten-DNA Assay (FT-HDA), relies on binding of anti-hapten antibodies/alkaline phosphatase conjugates to hapten-DNA, immobilized on disposable capillary absorbent filters, and visual detection of blue-grey coloured spots appearing on the filter after chromogenic reaction with enzyme substrates. FT-HDA of hapten-DNA is markedly faster and simpler than conventional diffusion assays on membranes.
Corti
A
A
Tecnogen SpA, Milan, Italy.
Tripputi
P
P
Cassani
G
G
D000469:Alkaline Phosphatase / Q000737:chemistry; D000818:Animals; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D004076:Digoxigenin; D005374:Filtration; D006241:Haptens / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D007118:Immunoassay / Q000379:methods*; D051379:Mice; D010957:Plasmids; D013450:Sulfones
D006241:Haptens; D013450:Sulfones; D004247:DNA; D000469:Alkaline Phosphatase; D004076:Digoxigenin