title: Human hepatic regenerative stimulator substance: partial purification and biological characterization of hepatic stimulator substance from human fetal liver cells.
abstract: Current support or replacement therapies for fulminant acute hepatic failure are frequently very disappointing. In this study, human hepatic stimulator substance--a liver-specific growth factor--was partially purified from human fetal liver cells and characterized by its biological effects. Almost 70-fold protein content was purified with an approximately 80-fold increase in specific growth stimulator activity. Human hepatic stimulator substance proved to be heat-stable, protease-sensitive, organ-specific and species-nonspecific. Human hepatic stimulator substance produced a two- to threefold increase of 3H-thymidine incorporation into hepatic DNA when injected intraperitoneally into growing weanling mice (nonhepatectomized) or regenerating rats (34% hepatectomy). The effects of hHSS in reversing the lethality of D-galactosamine (1.6 gm/kg body weight)-induced hepatic necrosis in rats were further evaluated. A survival rate of 4% (n = 24), 41% (n = 12, p less than 0.05), 33% (n = 12, p less than 0.05), 31% (n = 13, p less than 0.05) and 18% (n = 11, p greater than 0.05) was observed when the rats were injected with 4 ml of saline intraperitoneally, 4 ml of human intact fetal hepatocytes (2.4 x 10(8] intraperitoneally, 4 ml of human hepatic stimulator substance intraperitoneally, 2 ml of twofold concentrated human hepatic stimulator substance intravenously and 1 ml of fourfold human hepatic stimulator substance intramuscularly, respectively, 20 hr after poisoning.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Infectious Diseases, Second (TONGDU) Affiliated Hospital, Fourth Military Medical University, Xi'an, People's Republic of China.
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Yao
authors.forename: W S
authors.initials: WS
authors.lastname: Yang
authors.forename: W B
authors.initials: WB
authors.lastname: Zhang
authors.forename: Y N
authors.initials: YN
authors.lastname: Chen
authors.forename: F Y
authors.initials: FY
authors.lastname: Yang
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D056486:Chemical and Drug Induced Liver Injury; D005260:Female; D005333:Fetus / Q000378:metabolism*; D005688:Galactosamine; D006133:Growth Substances / Q000302:isolation & purification* / Q000494:pharmacology / Q000502:physiology; D006498:Hepatectomy; D006801:Humans; D008099:Liver / Q000166:cytology / Q000196:embryology* / Q000473:pathology; D008107:Liver Diseases / Q000401:mortality; D008115:Liver Regeneration; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009336:Necrosis; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D006133:Growth Substances; D005688:Galactosamine