Physical and genetic analyses of IncI2 plasmid R721: evidence for the presence of shufflon.
A physical map of the 75.1-kb IncI2 plasmid R721 was constructed by using 15 restriction enzymes, and the regions of several genetic determinants including the origins of replication and of conjugal DNA transfer were located on the physical map. It was found that R721 bears a DNA region which undergoes DNA rearrangement similar to the shufflon of R64.
Komano
T
T
Department of Biology, Tokyo Metropolitan University, Japan.
Fujitani
S
S
Funayama
N
N
Kanno
A
A
Sakuma
K
K
D003227:Conjugation, Genetic; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D015723:Gene Library; D005798:Genes, Bacterial; D011815:R Factors; D015183:Restriction Mapping
D004269:DNA, Bacterial