Distribution of Alzheimer's disease amyloid A4-generating enzymes in rat brain tissue.
To screen for putative amyloid A4-splitting proteinases, model peptide substrates homologous to the N- and C-terminal portions of the A4 protein were synthesized. The N-terminal A4-splitting enzyme activity was found to be higher in the hypothalamus and limbic area, compared with in the cerebral cortex and cerebellum in 2-month-old rat. The activity decreased throughout the brain in 20-month-old rat. On the other hand, the activity of the putative C-terminal A4-splitting enzyme was significantly higher, 1.4-fold, in the hippocampus than in the cerebral cortex in the young rat. The activity also significantly decreased in the brain of 20-month-old rat, but was still higher in the cerebral cortex, hippocampus and cerebellum.
Ishiura
S
S
National Institute of Neuroscience, NCNP, Tokyo, Japan.
Nishikawa
T
T
Tsukahara
T
T
Momoi
T
T
Ito
H
H
Suzuki
K
K
Sugita
H
H
D000375:Aging / Q000378:metabolism*; D000544:Alzheimer Disease / Q000201:enzymology*; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000254:growth & development; D010450:Endopeptidases / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D016229:Amyloid beta-Peptides; D010450:Endopeptidases