Gene targeting. How efficient can you get?
Capecchi
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D000818:Animals; D004252:DNA Mutational Analysis; D005818:Genetic Engineering / Q000379:methods*; D007895:Leishmania tropica / Q000235:genetics*; D011995:Recombination, Genetic; D014346:Trypanosoma brucei brucei / Q000235:genetics*