Importance of the G27-A43 mismatch at the anticodon stem of Escherichia coli tRNA(Thr2).
The tRNA(Thr2) isoacceptor of E. coli has a G-A mismatch at positions 27-43. When the anticodon of this tRNA was converted to an amber anticodon (CUA), this tRNA showed suppressor activity in E. coli. Moreover, introduction of the base pair (G-C or U-A) at positions 27-43 of this suppressor tRNA reduced its suppressor activity. These results indicate that the G27-A43 mismatch is necessary for full function of tRNA(Thr2).
Komine
Y
Y
Department of Biophysics, Faculty of Science, Kyoto University, Japan.
Inokuchi
H
H
D000926:Anticodon / Q000235:genetics*; D001482:Base Composition; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012363:RNA, Transfer, Thr / Q000235:genetics*; D015183:Restriction Mapping; D013489:Suppression, Genetic
D000926:Anticodon; D012363:RNA, Transfer, Thr