title: Estrous cycle dependent regulation of peptidylarginine deiminase transcripts in female rat pituitary.
abstract: Northern blot hybridization demonstrated 4.5-5.0 kilobase peptidylarginine deiminase mRNA in poly (A)+ RNA-enriched fractions of both male and female pituitaries. Dot blot analysis of total RNA fractions showed more than 50-fold sex difference in the mRNA content. The female pituitary mRNA content showed at least a 50-fold variation during the estrous cycle characterized by elevation at diestrus and proestrus followed by a rapid decline at estrus. The data were discussed in comparison with the sex difference and estrous cycle dependence of the pituitary enzyme content reported previously.
authors.affiliation: Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Hikichi
authors.forename: S
authors.initials: S
authors.lastname: Nagata
authors.forename: T
authors.initials: T
authors.lastname: Senshu
mesh_terms: D000818:Animals; D002531:Cerebellum / Q000201:enzymology; D004971:Estrus; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006867:Hydrolases / Q000235:genetics*; D008297:Male; D010902:Pituitary Gland / Q000201:enzymology*; D000080002:Protein-Arginine Deiminase Type 4; D000076342:Protein-Arginine Deiminases; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D012737:Sex Factors; D013116:Spinal Cord / Q000201:enzymology; D013363:Submandibular Gland / Q000201:enzymology; D014158:Transcription, Genetic; D014599:Uterus / Q000201:enzymology
chemical_list: D012333:RNA, Messenger; D006867:Hydrolases; D000080002:Protein-Arginine Deiminase Type 4; D000076342:Protein-Arginine Deiminases