title: Evaluation of a commercial radioimmunoassay kit for the detection of lysergide (LSD) in serum, whole blood, urine and stomach contents.
abstract: The Diagnostic Products Corporation Coat-A-Count radioimmunoassay kit for LSD in urine has been evaluated for use in forensic toxicology with a variety of sample types. The cut-offs (defined as the mean response of blank samples plus three standard deviation) for LSD in serum, haemolysed whole blood, urine and stomach contents were 0.06, 0.050-0.055, 0.18 and 0.18 ng/ml, respectively. Preliminary extraction of LSD from the samples is not usually necessary. The precision of the analysis and the recoveries from spiked samples were satisfactory. The cross-reactivities of 2-oxo-LSD, lysergic acid methyl propylamide, lysergic acid monoethylamide and nor-LSD were estimated to be 11,6,2 and 1% respectively relative to LSD (100%).
authors.affiliation: Metropolitan Police Forensic Science Laboratory, London, U.K.
authors.forename: D
authors.initials: D
authors.lastname: Altunkaya
authors.forename: R N
authors.initials: RN
authors.lastname: Smith
mesh_terms: D002138:Calibration; D005069:Evaluation Studies as Topic; D005554:Forensic Medicine / Q000379:methods*; D005766:Gastrointestinal Contents / Q000737:chemistry*; D006801:Humans; D008238:Lysergic Acid Diethylamide / Q000032:analysis* / Q000097:blood / Q000652:urine; D011863:Radioimmunoassay / Q000379:methods
chemical_list: D008238:Lysergic Acid Diethylamide