Chromosomal assignment of the human homologue encoding SGP-2.
Although originally characterized as a constitutively expressed gene product in mammalian Sertoli cells, sulfated glycoprotein-2 (SGP-2) has gained widespread attention due to its remarkably rapid and sizable induction in numerous types of mammalian cells undergoing apoptosis, or programmed death. In order to identify the chromosomal assignment for the human homologue of SGP-2, we performed Southern blot analysis of Bgl II restricted genomic DNA extracted from a panel of cloned hamster-human hybrid cell lines and screened for the presence of restriction fragments homologous to SGP-2. The results of this analysis indicate that the human homologue of SGP-2 resides on chromosome 8.
Slawin
K
K
Department of Urology, Columbia University College of Physicians and Surgeons, New York, NY 10032.
Sawczuk
I S
IS
Olsson
C A
CA
Buttyan
R
R
D000818:Animals; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D003001:Cloning, Molecular; D051152:Clusterin; D006224:Cricetinae; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology / Q000378:metabolism; D008297:Male; D018832:Molecular Chaperones; D009363:Neoplasm Proteins / Q000235:genetics; D009919:Orchiectomy; D011467:Prostate / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D015183:Restriction Mapping; D012708:Sertoli Cells / Q000378:metabolism
C495242:CLU protein, human; D051152:Clusterin; D006023:Glycoproteins; D018832:Molecular Chaperones; D009363:Neoplasm Proteins