title: Structural analysis of the specific capsular polysaccharide of Rhodococcus equi serotype 1.
abstract: The specific capsular polysaccharide produced by Rhodococcus equi serotype 1 was found to be a high molecular weight acidic polymer composed of D-glucose, D-mannose, and D-glucuronic acid. Structural analysis of the polysaccharide employed a combination of chemical and nuclear magnetic resonance techniques, from which it was determined that the polysaccharide possessed a linear repeating tetrasaccharide unit containing a single O-acetyl substituent and and acetal-linked pyruvic acid moiety: [formula: see text] The 1H and 13C nuclear magnetic resonances of O-deacetylated and pyruvic-free serotype 1 polysaccharides were fully assigned by homo- and hetero-nuclear chemical shift correlation methods.
authors.affiliation: Division of Biological Sciences, National Research Council of Canada, Ottawa, Ont.
authors.forename: R A
authors.initials: RA
authors.lastname: Leitch
authors.forename: J C
authors.initials: JC
authors.lastname: Richards
mesh_terms: D000107:Acetylation; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005947:Glucose / Q000032:analysis; D005965:Glucuronates / Q000032:analysis; D020723:Glucuronic Acid; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000032:analysis; D008745:Methylation; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D011773:Pyruvates / Q000032:analysis; D019289:Pyruvic Acid; D012240:Rhodococcus / Q000032:analysis*
chemical_list: D005965:Glucuronates; D011135:Polysaccharides, Bacterial; D011773:Pyruvates; D019289:Pyruvic Acid; D020723:Glucuronic Acid; D005947:Glucose; D008358:Mannose