Liquid chromatography/mass spectrometry of plasma glucose and secreted glucuronate for metabolic studies in humans.
Negative ion thermospray liquid chromatographic/mass spectrometric methods have been developed for the determination of isotopic enrichments for glucose and acetaminophen-uridine diphosphate glucuronate from (1-2H1)glucose, (1-2H1)galactose and (2-13C)acetate in humans. The error of estimate ranged from below 1% to 5%. The advantages of the method include fast analysis (up to 35 per hour), eased sample preparation, good precision, sensitivity comparable with gas chromatography/mass spectrometry and better than with isotope ratio mass spectrometry.
Reid
S
S
Clinical Mass Spectrometry Facility, Children's Hospital Oakland Research Institute, California 94609.
Shackleton
C
C
Wu
K
K
Kaempfer
S
S
Hellerstein
M K
MK
D001786:Blood Glucose / Q000032:analysis*; D002853:Chromatography, Liquid; D005965:Glucuronates / Q000097:blood*; D006801:Humans; D013058:Mass Spectrometry
D001786:Blood Glucose; D005965:Glucuronates