Electron microscopy, plaque assay and preliminary serological characterization of three ectromelia virus strains isolated in Poland in the period 1986-1988.
Three strains of viruses have been identified as ectromedia virus (EV) based on their origin, clinical features, morphology, size of virions (140 x 220 nm), replicative ability and specific cytoplasmatic fluorescence. The mean diameter of plaques produced by EV strains was 0.76 mm (range 0.5-1.0 mm). The neutralizing properties of the tested sera were evaluated by seroneutralization (SN) and hemagglutination inhibition (HAI) tests.
Spohr de Faundez
I
I
Department of Microbiology, Faculty of Veterinary Medicine, Warsaw Agricultural University, Poland.
Niemialtowski
M
M
Malicka
E
E
Malicki
K
K
Gieryńska
M
M
Popis
A
A
D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D004481:Ectromelia virus / Q000302:isolation & purification / Q000472:pathogenicity / Q000648:ultrastructure*; D004482:Ectromelia, Infectious / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D006385:Hemagglutination Inhibition Tests; D051379:Mice; D009500:Neutralization Tests; D011044:Poland; D011817:Rabbits; D010948:Viral Plaque Assay; D014779:Virus Replication