Labelling autologous platelets with 111In tropolonate for platelet kinetic studies: limitations imposed by thrombocytopenia.
An in vitro method of radiolabelling platelets with 111In tropolonate in plasma has been devised enabling imaging and cell kinetic studies to be performed in patients with thrombocytopenia (TP) using autologous, rather than donor, platelets. Platelets from 10 TP patients, with platelet counts ranging from 4-91 x 10(9)/l, were labelled in 50% plasma with 111In tropolonate, containing the optimum tropolone concentration of 2 x 10(-4) mol/l, at platelet concentrations ranging from 0.08-4.5 x 10(9)/ml resulting in labelling efficiencies (LE) between 51 and 86%. In 4 patients, red cells contaminated the platelets but this was corrected for by measuring the proportion of 111In on the platelets prior to injection and in the post-injection blood samples. Platelet recovery (PR), mean platelet life-span (MPLS) and platelet production rate (PPR) were calculated and splenic and hepatic images were taken. The results clearly show that useful clinical data can be obtained by this method even in patients with severe TP.
Danpure
H J
HJ
MRC Cyclotron Unit, Hammersmith Hospital, London, U.K.
Osman
S
S
Peters
A M
AM
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001792:Blood Platelets / Q000473:pathology / Q000502:physiology*; D002469:Cell Separation; D002470:Cell Survival; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007205:Indium Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D007700:Kinetics; D008099:Liver / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009942:Organometallic Compounds; D010976:Platelet Count; D013154:Spleen / Q000473:pathology; D013921:Thrombocytopenia / Q000097:blood*; D014334:Tropolone / Q000031:analogs & derivatives*
D007205:Indium Radioisotopes; D009942:Organometallic Compounds; C043895:indium tris(tropolonate); D014334:Tropolone