Characterisation of Chlamydia psittaci isolated from a horse.
This paper describes the isolation and characterisation of a strain of Chlamydia psittaci obtained from a nasal swab taken from a horse with serous nasal discharge. Initial isolation was achieved in cycloheximide-treated McCoy cell monolayers. Chlamydial inclusions stained by immunofluorescence either with a rabbit antiserum raised against C. psittaci or with a monoclonal antibody directed against the genus-specific lipopolysaccharide antigen were single and compact. They did not stain with iodine or with a monoclonal antibody reactive against Chlamydia trachomatis. The agent was re-isolated in the yolk sacs of embryonated hens eggs and designated N16. Identification of the agent was confirmed by electron microscopy. Unique plasmid DNA was prepared from a purified suspension of chlamydial elementary bodies (EBs), and analysed by electrophoresis through 1.0% agarose gels stained by ethidium bromide. This strain of C. psittaci grew relatively slowly in cycloheximide-treated McCoy cells, and the yield of elementary bodies during the course of one growth cycle was relatively low.
Wills
J M
JM
Department of Medical Microbiology, University of Manchester Medical School, Great Britain.
Watson
G
G
Lusher
M
M
Mair
T S
TS
Wood
D
D
Richmond
S J
SJ
D000818:Animals; D002460:Cell Line; D002498:Centrifugation; D002691:Chlamydophila psittaci / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure; D004269:DNA, Bacterial / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D008297:Male; D008854:Microscopy, Electron; D009297:Nasal Mucosa / Q000382:microbiology; D010957:Plasmids; D009956:Psittacosis / Q000382:microbiology / Q000662:veterinary*; D012141:Respiratory Tract Infections / Q000382:microbiology / Q000662:veterinary*
D004269:DNA, Bacterial