[The cloning of "residual" DNA and the determination of the primary structure for the fragments of the barley-specific family of tandem repetitive sequences].
High molecular weight "relic" DNA fraction can be separated from the bulk of barley DNA digested with different restriction enzymes by agarose gel electrophoresis. The majority of AluI-relic DNA clones contained barley simple sequence satellite DNA and other families of repetitive DNA. The clones representing HvRT family were sequenced. This family was found to be barley-specific. It is composed of tandemly arranged 118-bp monomers and presents in 7 x 10(5) copies in barley genome.
Belostotskiĭ
D A
DA
Mil'shina
N V
NV
Anan'ev
E V
EV
D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000235:genetics*; D001467:Hordeum / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D015183:Restriction Mapping
D004247:DNA
Klonirovanie "ostatochnoĭ" DNK i opredelenie pervichnoĭ struktury fragmentov iachmen'-spetsificheskogo semeĭstva tandemno povtoriaiushchikhsia posledovatel'nosteĭ.