Location of the cis-acting auxin-responsive region in the promoter of the par gene from tobacco mesophyll protoplasts.
We have isolated a genomic clone of an auxin-regulated par gene, which is expressed during the transition from G0 phase to S phase in the early stage of tobacco mesophyll protoplasts cultured in vitro, from a tobacco genomic library using the par cDNA as a probe. When a chimeric gene, in which a reporter gene for bacterial beta-glucuronidase (GUS) was placed downstream of the 5' flanking sequences of the par gene, was introduced into tobacco mesophyll protoplasts by electroporation, the chimeric gene elicited auxin-regulated expression of GUS activity. Because deletion of a 111-base-pair (bp) direct repeat in the 5' flanking sequences of the par gene abolished the auxin-induced GUS activity, it is deduced that in the 111-bp direct repeat of the par gene promoter is localized an auxin-responsive region, which regulates auxin-mediated activation of transcription.
Takahashi
Y
Y
Department of Biology, Faculty of Science, Nagoya University, Japan.
Niwa
Y
Y
Machida
Y
Y
Nagata
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D002678:Chimera; D005786:Gene Expression Regulation / Q000187:drug effects*; D015698:Genomic Library; D007210:Indoleacetic Acids / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D011523:Protoplasts / Q000378:metabolism; D016385:TATA Box; D014026:Nicotiana / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D007210:Indoleacetic Acids