High-performance liquid chromatographic bio-analysis and preliminary pharmacokinetics of the experimental antitumour drug vintriptol.
A high-performance liquid chromatographic procedure, including sample pretreatment, is presented for the analysis of the experimental antitumour drug vintriptol in plasma. The sample pretreatment involved liquid-liquid extraction of the buffered (pH 3) sample with chloroform. Vinblastine was used as internal standard. Separation was achieved on a Hypersil ODS (5 microns) column with a mobile phase of acetonitrile-phosphate buffer. Electrochemical detection (at +0.70 V) was used, giving a detection limit of 2 micrograms/l. The applicability of the assay was demonstrated in a pharmacokinetic study with eight cancer patients who received 45 or 50 mg/m2 vintriptol in a phase I study. A three-compartment model was used to fit the plasma concentration-time curves. Pharmacokinetic parameters are presented.
van Tellingen
O
O
Department of Clinical Chemistry, Netherlands Cancer Institute, Amsterdam.
van der Woude
H R
HR
Beijnen
J H
JH
Rao
K S
KS
ten Bokkel Huinink
W W
WW
Nooyen
W J
WJ
D000970:Antineoplastic Agents / Q000097:blood / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006207:Half-Life; D006801:Humans; D007262:Infusions, Intravenous; D011786:Quality Control; D014747:Vinblastine / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics
D000970:Antineoplastic Agents; C050339:vintriptol; D014747:Vinblastine