Selective binding of a 30-kilodalton protein to disposable hydrophilic contact lenses.
To investigate the accumulation of tear proteins on disposable extended-wear contact lenses (42% Etafilcon A and 58% hydration), a technique involving sodium dodecyl sulfate-polyacrylamide minigel electrophoresis combined with a sensitive silver-staining method was used. Besides the binding of large amounts of tear lysozyme the authors found an accumulation of an as yet unidentified 30-kilodalton (kD) protein. Longitudinal experiments showed lysozyme binding after 1 day of lens wear. The 30-kD protein was detected after a 2-day wearing period. The fact that protein deposition occurs during the relatively short wearing period of these lenses (1 week) may explain the unexpectedly high incidence of contact lens-associated conjunctivitis observed with these lenses.
Cheng
K H
KH
Department of Ophthalmology, University of Amsterdam, The Netherlands.
Kok
J H
JH
van Mil
C
C
Kijlstra
A
A
D003262:Contact Lenses, Extended-Wear; D004209:Disposable Equipment; D004591:Electrophoresis, Polyacrylamide Gel; D005136:Eye Proteins / Q000378:metabolism*; D006801:Humans; D009113:Muramidase / Q000378:metabolism; D011485:Protein Binding; D013666:Tears / Q000378:metabolism*; D013997:Time Factors
D005136:Eye Proteins; D009113:Muramidase