[Possibilities for individual identification of hair roots using DNA probes].
High-molecular weight DNA was extracted from hair roots. The obtained DNA was digested with Hae III and Msp I and transferred to nylon membranes by Southern blotting. The possibility of applying a probe for sex determination (pHY2.1) a "single-locus"-probe (YNH24) as well as a "multi-locus"-probe (M13) was examinated as a function of the amount of hair being supplied. In the laboratory experiment a single hair root was enough for a sex determination or individual identification. Because of methodical difficulties in practice this kind of profiling should only be done with several hair roots.
Haas
H
H
Institut für Rechtsmedizin, Universität zu Köln.
Prinz
M
M
Ohshima
T
T
Staak
M
M
Berghaus
G
G
D000293:Adolescent; D000328:Adult; D002874:Chromosome Mapping / Q000379:methods*; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D006197:Hair / Q000737:chemistry*; D006801:Humans; D008297:Male; D012732:Sex Determination Analysis
D015342:DNA Probes; D005819:Genetic Markers; D004247:DNA
Möglichkeiten einer Individualisierung an Haarwurzeln mittels DNA-Sonden.