Effect of dietary lactose on Salmonella colonization of market-age broiler chickens.
The effect of providing lactose in feed and inoculation with volatile fatty acid-producing anaerobic cultures (AC) of cecal flora on Salmonella typhimurium colonization was evaluated in broilers. One-day-old chicks were divided into four groups and provided 1) no lactose, no AC; 2) AC, no lactose; 3) AC and lactose on days 1-10; or 4) AC and lactose on days 1-40. All groups were challenged per os with 10(6) Salmonella on day 3 and with 10(8) Salmonella on day 33. Salmonella growth in the cecal contents was significantly decreased (P less than 0.01) on day 10 in the chicks provided lactose from day 1-10. However, after the removal of lactose from the diet, the chicks were susceptible to Salmonella colonization. The number of Salmonella in the ceca was significantly reduced (P less than 0.05) in the chicks provided lactose throughout the 40-day growing period. Dietary lactose decreased the pH of the cecal contents and was accompanied by marked increases in the concentrations of undissociated bacteriostatic volatile fatty acids in the cecal contents.
Corrier
D E
DE
U.S. Department of Agriculture, Veterinary Toxicology and Entomology Research Laboratory, College Station, Texas 77840.
Hinton
A
A
Ziprin
R L
RL
DeLoach
J R
JR
D000818:Animals; D001421:Bacteria, Anaerobic / Q000254:growth & development; D001835:Body Weight / Q000187:drug effects; D002353:Carrier State / Q000382:microbiology / Q000662:veterinary; D002432:Cecum / Q000737:chemistry / Q000187:drug effects / Q000382:microbiology; D002645:Chickens; D004040:Dietary Carbohydrates / Q000008:administration & dosage / Q000494:pharmacology; D005232:Fatty Acids, Volatile / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D007785:Lactose / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D011201:Poultry Diseases / Q000382:microbiology*; D011897:Random Allocation; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000254:growth & development*
D004040:Dietary Carbohydrates; D005232:Fatty Acids, Volatile; D007773:Lactates; D019344:Lactic Acid; D007785:Lactose