Microencapsulation of single, multiple, and zona pellucida-free mouse preimplantation embryos in sodium alginate and their development in vitro.
Preimplantation embryos obtained from immature superovulated B6D2F1 female mice were microencapsulated in sodium alginate singly, in multiples of 2 or 3, or denuded of their zona pellucida. Encapsulated embryos developed in vitro at a rate similar to control embryos. Development of zona pellucida-free embryos was significantly less than that of intact embryos, but there was no difference between encapsulated and non-encapsulated zona pellucida-free embryos. Development of 2- and 4-cell embryos in sodium alginate was independent of cell stage. This report demonstrates the usefulness of a viable, biodegradable embedding material for the microencapsulation of manipulated preimplantation mammalian embryos.
Cosby
N C
NC
Endocrine Research Center, Michigan State University, East Lansing 48824.
Dukelow
W R
WR
D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000502:physiology*; D002478:Cells, Cultured; D003470:Culture Media; D005314:Embryonic and Fetal Development; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D051379:Mice; D008815:Mice, Inbred Strains; D008846:Micromanipulation / Q000379:methods; D015044:Zona Pellucida / Q000502:physiology
D000464:Alginates; D003470:Culture Media; D006603:Hexuronic Acids; D020723:Glucuronic Acid