Synthesis and secretion of active lipoprotein lipase in Chinese-hamster ovary (CHO) cells.
Cultured Chinese-hamster ovary cells (CHO cells) were found to produce and secrete a lipase, which was identified as a lipoprotein lipase by the following criteria. Its activity was stimulated by serum and apolipoprotein CII, and was inhibited by high salt concentration. The lipase bound to heparin-agarose and co-eluted with 125I-labelled bovine lipoprotein lipase in a salt gradient. A chicken antiserum to bovine lipoprotein lipase inhibited the activity and precipitated a labelled protein of the same apparent size as bovine lipoprotein lipase from media of CHO cells labelled with [35S]methionine. The lipase activity and secretion were similar in growing cells and in cells that had reached confluency. Hence, lipoprotein lipase appears to be expressed constitutively in CHO cells and is not linked to certain growth conditions, as in pre-adipocyte and macrophage cell lines. At 37 degrees C, but not at 4 degrees C, heparin increased the release of lipase to the medium 2-4-fold. This increased release occurred without depletion of cell-associated lipase activity, suggesting that heparin enhanced release of newly synthesized lipase.
Rojas
C
C
Department of Medical Biochemistry and Biophysics, University of Umeå, Sweden.
Enerbäck
S
S
Bengtsson-Olivecrona
G
G
D000818:Animals; D053304:Apolipoprotein C-II; D001056:Apolipoproteins C / Q000494:pharmacology; D001769:Blood; D002417:Cattle; D002460:Cell Line; D002846:Chromatography, Affinity; D006224:Cricetinae; D005260:Female; D006493:Heparin / Q000494:pharmacology; D007106:Immune Sera / Q000494:pharmacology; D007163:Immunosorbent Techniques; D008071:Lipoprotein Lipase / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000378:metabolism; D010053:Ovary; D012965:Sodium Chloride / Q000494:pharmacology
D053304:Apolipoprotein C-II; D001056:Apolipoproteins C; D007106:Immune Sera; D012965:Sodium Chloride; D006493:Heparin; D008071:Lipoprotein Lipase