Determination of taxonomic resolution capacity of conventional one-dimensional SDS-polyacrylamide gel electrophoresis of whole-cell proteins using Enterobacteriaceae.
The capacity of one-dimensional SDS-PAGE of whole bacterial cells to both separate and cluster taxonomic units is studied using members of Enterobacteriaceae as test material. The results show that intraspecies variation can be detected and on the other hand the degree of taxonomic divergence which still can be grouped together is determined. In addition the system has high tolerance to changes in cell culture conditions making the usage of SDS-PAGE suitable for applications where rapid and reliable bacterial identification is needed.
Hantula
J
J
Department of Genetics, University of Helsinki, Finland.
Korhonen
T K
TK
Bamford
D H
DH
D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004755:Enterobacteriaceae / Q000145:classification* / Q000235:genetics / Q000254:growth & development; D014644:Genetic Variation; D012967:Sodium Dodecyl Sulfate; D009626:Terminology as Topic
D001426:Bacterial Proteins; D012967:Sodium Dodecyl Sulfate