Effect of ciprofloxacin on experimental osteomyelitis in the rabbit tibia, induced with a mixed infection of Staphylococcus epidermidis and Bacteroides thetaiotaomicron.
After induction of experimental polymicrobic osteomyelitis with Staphylococcus epidermidis and Bacteroides thetaiotaomicron (ciprofloxacin MIC, 0.5 micrograms/ml and 4.0 micrograms/ml, respectively), in the presence of a foreign body implant, in a rabbit tibia model, ciprofloxacin was administered to infected animals for 2- and 4-week periods. At necropsy, rabbits in the 2-weeks-treated group had mean ciprofloxacin levels of 5.94 micrograms/ml in serum, 3.63 micrograms/g in marrow, and 1.88 micrograms/g in bone. Rabbits in the 4-weeks-treated group had mean ciprofloxacin levels of 7.77 micrograms/ml in serum, 5.84 micrograms/g in marrow, and 2.01 micrograms/g in bone. Quantitative bacterial plate counts were conducted on weighed samples of infected bone, marrow, and the catheter implant, taken at necropsy from treated and control rabbits. Variable reduction of bacterial numbers was observed in samples from treated animals, as compared to untreated controls. Samples of infected bone, marrow and catheter, showed comparable evidence of osteomyelitis and bacterial colonization in both treated and control animals. Although relatively high tissue levels of ciprofloxacin were attained, little therapeutic effect was observed.
Mayberry-Carson
K J
KJ
Department of Microbiology, James H. Quillen College of Medicine, Johnson City, Tennessee.
Tober-Meyer
B
B
Gill
L R
LR
Lambe
D W
DW
Hossler
F E
FE
D000818:Animals; D001439:Bacteroides / Q000187:drug effects; D001442:Bacteroides Infections / Q000150:complications / Q000188:drug therapy*; D001853:Bone Marrow / Q000378:metabolism / Q000648:ultrastructure; D001842:Bone and Bones / Q000378:metabolism / Q000648:ultrastructure; D002408:Catheters, Indwelling; D002939:Ciprofloxacin / Q000097:blood / Q000493:pharmacokinetics / Q000627:therapeutic use*; D004195:Disease Models, Animal; D008297:Male; D008854:Microscopy, Electron; D010019:Osteomyelitis / Q000188:drug therapy*; D011817:Rabbits; D013203:Staphylococcal Infections / Q000150:complications / Q000188:drug therapy*; D013212:Staphylococcus epidermidis / Q000187:drug effects*; D013977:Tibia; D014018:Tissue Distribution
D002939:Ciprofloxacin