[Nuclear DNA content in the cells of squamous cell carcinoma and Bowen's disease. II. A study on DNA contents of deeply invaded cells in comparison with non-invading cells at the upper dermis].
To know the variation of DNA contents of SCC cells due to invasion activity, cytophotometric assay was used. The specimens for this study were paraffin-embedded tissue section of SCC stained with azocarmin G for blocking the non-specific fluorescence. By comparing the nuclear DNA content of tumor cells at the upper dermis and at the subcutaneous fat tissue, it was found that polyploid cells (greater than 4C and greater than 6C) populations in deep tissue was significantly higher than those in the upper dermis. This finding suggests that SCC cells capable to invade deeply are having larger amount of nuclear DNA that cells at the upper dermis.
Toyoshima
H
H
Department of Dermatology, Nagasaki University School of Medicine.
D000368:Aged; D000369:Aged, 80 and over; D001913:Bowen's Disease / Q000378:metabolism* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000473:pathology; D002467:Cell Nucleus / Q000737:chemistry; D004273:DNA, Neoplasm / Q000032:analysis*; D006801:Humans; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D011123:Polyploidy; D012867:Skin / Q000737:chemistry* / Q000473:pathology; D012878:Skin Neoplasms / Q000737:chemistry* / Q000473:pathology
D004273:DNA, Neoplasm