title: Description of Bacillus naganoensis sp. nov.
abstract: A new species, Bacillus naganoensis, is proposed for an obligately aerobic, moderately acidophilic, endospore-forming bacterium that produces a thermostable, aciduric pullulanase (EC 3.2.1.41). The organism was isolated from soil by selection on solid, pullulan-containing medium at pH 4.0 and 30 degrees C. The isolate required a medium pH of less than 6.5 for growth initiation. Fatty acid composition studies revealed that the major fatty acid of cells grown in nutrient broth supplemented with 1% starch was 14-methylpentadecanoic acid (iso-C16) at 45 mol%. The guanine-plus-cytosine content of the DNA of this organism was 45 +/- 2 mol%. A type culture has been deposited with the American Type Culture Collection, Rockville, Md., as strain ATCC 53909.
authors.affiliation: Enzyme Bio-Systems Ltd., Arlington Heights, Illinois 60005.
authors.forename: E
authors.initials: E
authors.lastname: Tomimura
authors.forename: N W
authors.initials: NW
authors.lastname: Zeman
authors.forename: J R
authors.initials: JR
authors.lastname: Frankiewicz
authors.forename: W M
authors.initials: WM
authors.lastname: Teague
mesh_terms: D001407:Bacillus / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D004795:Enzyme Stability; D005227:Fatty Acids / Q000032:analysis; D006026:Glycoside Hydrolases / Q000096:biosynthesis; D006863:Hydrogen-Ion Concentration; D012988:Soil Microbiology; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006026:Glycoside Hydrolases; C100181:pullulanase