[The purification of tick-borne encephalitis virus preparations of cellular DNA].
According to the WHO requirements, the concentration of cellular DNA in vaccine preparations produced by pooling virus from continuous cell lines is limited to 100 ng/dose. In this study, different methods were used for purification of tick-borne encephalitis virus suspensions grown in continuous cultures of cell line 4647 from cellular DNA. Two approaches are proposed based on treatment with DNAse and promamin sulfate which allow one to reduce cellular DNA concentration in the virus preparation to the acceptable level. Prospects of their use in vaccine production are discussed.
El'bert
L B
LB
Terletskaia
E N
EN
Timofeev
A V
AV
Amosenko
F A
FA
Khapchaev
Iu Kh
IuKh
Mironova
L L
LL
Svitkin
Iu V
IuV
Vorovich
M F
MF
Lisitsyna
E A
EA
D000818:Animals; D002499:Centrifugation, Density Gradient / Q000379:methods; D002500:Centrifugation, Isopycnic / Q000379:methods; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D004247:DNA / Q000032:analysis / Q000302:isolation & purification*; D003850:Deoxyribonuclease I / Q000494:pharmacology; D004669:Encephalitis Viruses, Tick-Borne / Q000187:drug effects / Q000302:isolation & purification*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D008851:Micropore Filters; D011479:Protamines / Q000494:pharmacology; D015164:Vaccines, Inactivated / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification; D014765:Viral Vaccines / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification
D011479:Protamines; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; D004247:DNA; D003850:Deoxyribonuclease I
Ochistka preparatov virusa kleshchevogo éntsefalita ot kletochnoĭ DNK.