Primary structure of a base non-specific and adenylic acid preferential ribonuclease from Aspergillus saitoi.
The complete primary structure of a base non-specific and adenylic acid preferential RNase (RNase M) from Aspergillus saitoi was determined. The sequence was determined by analysis of the peptides generated by digestion of heat-denatured RNase M with lysylendopeptidase, and the peptides generated from RCM RNase M by digestion with staphylococcal V8 protease or chemical cleavage with BrCN. It consisted of 238 amino acid residues and carbohydrate moiety attached to the 74th asparagine residue. The molecular weight of the protein moiety deduced from the sequence was 26,596. The locations of 10 half cystine residues are almost superimposable on those of RNase Rh from Rhizopus niveus and RNase T2 from Aspergillus oryzae which have similar base specificity. The homology between RNase M and RNase Rh and RNase T2 amounted to 97 and 160 amino acid residues, respectively. The amino acid sequences conserved in the three RNases are concentrated around the three histidine residues, which are supposed to form part of the active sites of these RNases.
Watanabe
H
H
Department of Microbiology, Hoshi College of Pharmacy, Tokyo.
Naitoh
A
A
Suyama
Y
Y
Inokuchi
N
N
Shimada
H
H
Koyama
T
T
Ohgi
K
K
Irie
M
M
D000249:Adenosine Monophosphate / Q000378:metabolism*; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001230:Aspergillus / Q000201:enzymology*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D003488:Cyanogen Bromide; D010450:Endopeptidases; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis; D011489:Protein Denaturation; D012260:Ribonucleases / Q000032:analysis* / Q000737:chemistry; D013045:Species Specificity; D009626:Terminology as Topic; D014364:Tryptophan / Q000032:analysis
D000596:Amino Acids; D007202:Indicators and Reagents; D010455:Peptides; D000249:Adenosine Monophosphate; D014364:Tryptophan; D012260:Ribonucleases; D010450:Endopeptidases; D003488:Cyanogen Bromide