Effects of metoclopramide on rat spleen lymphocyte proliferation.
The effect of metoclopramide on proliferation of spleen lymphocytes in rats was examined. The rate of [3H]thymidine incorporation into DNA was used as an index of lymphocytes proliferation. It was shown that the incorporation of [3H]thymidine into DNA of spleen lymphocytes in the rats treated with metoclopramide was significantly higher than that in the control group. The effects of various concentrations of prolactin and metoclopramide on [3H]thymidine uptake by DNA of spleen lymphocytes was also studied in vitro. It was shown that: 1, Prolactin, at the concentration of 1 and 0.1 ng/ml significantly increased [3H]thymidine incorporation into DNA of spleen lymphocytes. Metoclopramide in all the examined concentrations also caused a significant proliferogenic effect on lymphocytes.
Zerek-Melen
G
G
Department of Experimental Endocrinology and Hormone Diagnostic, Institute of Endocrinology, Medical Academy of Lodz, Poland.
Winczyk
K
K
Kunert-Radek
J
J
Pawlikowski
M
M
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D008787:Metoclopramide / Q000494:pharmacology*; D011388:Prolactin / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D013154:Spleen / Q000187:drug effects / Q000276:immunology*; D013936:Thymidine / Q000378:metabolism
D003907:Dexamethasone; D011388:Prolactin; D004247:DNA; D008787:Metoclopramide; D013936:Thymidine