Borate G-banding technique applicable to in situ deoxyribonucleic acid/deoxyribonucleic acid hybridized chicken chromosomes.
The borate G-banding technique produced excellent longitudinal bands similar to trypsin G-bands on chicken chromosomes prepared from embryo material. Aged chromosome preparations on slides were pretreated in borate buffer (pH 9.2) for 10 to 30 s at 37 C, then stained in Giemsa solution. This simple G-banding procedure gave high-resolution chromosome banding and permitted the visualization of silver grains that localized cloned gene sequences or cloned DNA fragments to specific regions by in situ hybridization.
Shaw
E M
EM
Department of Animal Science, University of Minnesota, St. Paul 55108.
Otis
J S
JS
Guise
K S
KS
Shoffner
R N
RN
D000818:Animals; D001881:Borates; D002645:Chickens / Q000097:blood / Q000235:genetics*; D002871:Chromosome Banding / Q000379:methods*; D002875:Chromosomes; D004247:DNA / Q000032:analysis; D007962:Leukocytes / Q000648:ultrastructure; D008677:Metaphase; D009693:Nucleic Acid Hybridization
D001881:Borates; D004247:DNA