Quantitative autoradiographical analysis of the age-related modulation of central dopamine D1 and D2 receptors.
Quantitative autoradiography of [3H]SCH 23390 and [3H](-)-sulpiride binding was performed in the brain of rats of various ages (3, 11 and 24 months) in order to study the changes in D1 and D2 receptor density with age. Binding of [3H]SCH 23390 in the caudate-putamen decreased progressively and markedly at rostral levels in 11- and 24- compared with 3-month-old rats (max. decrease -63%) while at caudal levels significant decrease was observed only in 24-month-old rats. [3H](-)-Sulpiride binding progressively decreased during aging in the caudate-putamen at rostral levels and the decrease was more pronounced laterally (-70% at 24 months), while at caudal levels no significant decrease was observed. D1 and D2 binding sites also decreased in the nucleus accumbens and olfactory tubercle of aged rats, while in the substantia nigra only the D1 receptors appeared to be modified with aging. No change was found in the entopeduncular nucleus, amygdala, frontoparietal, suprarinal-prefrontal and anterior cingulate cortex. The results indicate that the age-associated decrease of D1 and D2 receptors is not widespread, being confined to dopaminergic areas with high density of dopamine receptors.
Morelli
M
M
Institute of Experimental Pharmacology and Toxicology, University of Cagliari, Italy.
Mennini
T
T
Cagnotto
A
A
Toffano
G
G
Di Chiara
G
G
D000375:Aging / Q000502:physiology*; D000818:Animals; D001345:Autoradiography; D001552:Benzazepines / Q000494:pharmacology; D001921:Brain / Q000502:physiology*; D001923:Brain Chemistry; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D011954:Receptors, Dopamine / Q000502:physiology*; D013378:Substantia Nigra / Q000378:metabolism / Q000502:physiology; D013469:Sulpiride / Q000494:pharmacology
D001552:Benzazepines; D011954:Receptors, Dopamine; D013469:Sulpiride