title: The primary structure of iodopsin, a chicken red-sensitive cone pigment.
abstract: A purified iodopsin was digested by CNBr or several proteolytic enzymes into fragments, the amino acid sequences of which were determined. A partial sequence of the C-terminal fragment was utilized for synthesizing an oligonucleotide probe which identified the iodopsin cDNA (1339 bases). The deduced amino acid sequence (362 residues) had 80%, 42%, or 43% homology to that of human red-sensitive cone pigment, cattle or chicken rhodospin, respectively. Although the hydropathy profile implies that iodopsin, like rhodopsin, has 7 transmembrane alpha-helical segments, iodopsin may have a hydrophilic pocket near the seventh segment on the basis of the unexpected cleavages in the middle of the segment VII by chymotrypsin under nondenaturing conditions.
authors.affiliation: Department of Biophysics, Faculty of Science, Kyoto University, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Kuwata
authors.forename: Y
authors.initials: Y
authors.lastname: Imamoto
authors.forename: T
authors.initials: T
authors.lastname: Okano
authors.forename: K
authors.initials: K
authors.lastname: Kokame
authors.forename: D
authors.initials: D
authors.lastname: Kojima
authors.forename: H
authors.initials: H
authors.lastname: Matsumoto
authors.forename: A
authors.initials: A
authors.lastname: Morodome
authors.forename: Y
authors.initials: Y
authors.lastname: Fukada
authors.forename: Y
authors.initials: Y
authors.lastname: Shichida
authors.forename: K
authors.initials: K
authors.lastname: Yasuda
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003488:Cyanogen Bromide; D004247:DNA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D010446:Peptide Fragments / Q000737:chemistry; D011487:Protein Conformation; D012168:Retinal Pigments / Q000737:chemistry* / Q000235:genetics; D012243:Rhodopsin / Q000737:chemistry; D017299:Rod Opsins; D012689:Sequence Homology, Nucleic Acid
chemical_list: D015345:Oligonucleotide Probes; D010446:Peptide Fragments; D012168:Retinal Pigments; D017299:Rod Opsins; C034941:iodopsin; D004247:DNA; D012243:Rhodopsin; D003488:Cyanogen Bromide