title: Differentiation of genes coding for Escherichia coli verotoxin 2 and the verotoxin associated with porcine edema disease (VTe) by the polymerase chain reaction.
abstract: Two sets of synthetic oligonucleotide primers were used in a polymerase chain reaction adaptation to distinguish the closely related genes for type 2 verotoxin (VT2 or Shiga-like toxin [SLT-II]) and the verotoxin associated with porcine edema disease (VTe or SLT-II variant [SLT-IIv]) in Escherichia coli.
authors.affiliation: National Laboratory for Enteric Pathogens, Laboratory Centre for Disease Control, Ottawa, Ontario, Canada.
authors.forename: W M
authors.initials: WM
authors.lastname: Johnson
authors.forename: D R
authors.initials: DR
authors.lastname: Pollard
authors.forename: H
authors.initials: H
authors.lastname: Lior
authors.forename: S D
authors.initials: SD
authors.lastname: Tyler
authors.forename: K R
authors.initials: KR
authors.lastname: Rozee
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics*; D015342:DNA Probes; D004487:Edema / Q000209:etiology / Q000662:veterinary; D004926:Escherichia coli / Q000235:genetics*; D004927:Escherichia coli Infections / Q000209:etiology / Q000662:veterinary; D005798:Genes, Bacterial; D016133:Polymerase Chain Reaction; D022622:Shiga Toxin 1; D013552:Swine; D013553:Swine Diseases / Q000209:etiology
chemical_list: D001427:Bacterial Toxins; D015342:DNA Probes; D022622:Shiga Toxin 1