Isolation of three allergenic fractions of the major allergen from Olea europea pollen and N-terminal amino acid sequence.
A method to isolate the major allergen from olive pollen (Ole e I) in high yield is described. The allergenic fraction has been separated into 3 subfractions by reverse-phase HPLC. All these fractions were reactive to allergic sera from olive-sensitized patients, giving similar responses. No significant differences were observed between the amino acid compositions of these three proteins. The amino acid sequence of the first 27 amino acid residues from the N-terminal end is given. No homologies have been detected between Ole e I and other known allergens obtained from pollen.
Villalba
M
M
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad Complutense, Madrid, Spain.
López-Otín
C
C
Martín-Orozco
E
E
Monsalve
R I
RI
Palomino
P
P
Lahoz
C
C
Rodríguez
R
R
D000485:Allergens / Q000737:chemistry / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006967:Hypersensitivity; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000302:isolation & purification; D011058:Pollen / Q000032:analysis*; D014197:Trees
D000485:Allergens; D010446:Peptide Fragments