title: Imaging of tumor in patients with indium-111-labeled biotin and streptavidin-conjugated antibodies: preliminary communication.
abstract: Tumor localization in patients has been achieved through the in vivo use of streptavidin and biotin. In these preliminary studies, the monoclonal antibody HMFG1 was conjugated with streptavidin and 1 mg was administered intravenously to each of 10 patients with documented squamous cell carcinoma of the lung. Two to 3 days later, 111In-labeled biotin was also administered intravenously. No evidence of toxicity was observed. Background radioactivity levels were reduced in liver (1% ID at 24 hr) and kidneys (2%) and in all other normal tissues and blood. Images of lung tumor were obtained in as little as 2 hr following administration of labeled biotin. In eight patients, tumor was detected with labeled biotin alone without the previous administration of streptavidin-conjugated antibody but in three of these patients, the images were improved with the prior administration of conjugated antibody. These results suggest that this approach may improve the tumor-to-normal tissue radioactivity ratios in radioimmunotargeting.
authors.affiliation: Royal Postgraduate Medical School, Hammersmith Hospital, London, United Kingdom.
authors.forename: H P
authors.initials: HP
authors.lastname: Kalofonos
authors.forename: M
authors.initials: M
authors.lastname: Rusckowski
authors.forename: D A
authors.initials: DA
authors.lastname: Siebecker
authors.forename: G B
authors.initials: GB
authors.lastname: Sivolapenko
authors.forename: D
authors.initials: D
authors.lastname: Snook
authors.forename: J P
authors.initials: JP
authors.lastname: Lavender
authors.forename: A A
authors.initials: AA
authors.lastname: Epenetos
authors.forename: D J
authors.initials: DJ
authors.lastname: Hnatowich
mesh_terms: D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins / Q000097:blood; D001710:Biotin / Q000097:blood; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000000981:diagnostic imaging*; D006801:Humans; D007205:Indium Radioisotopes / Q000097:blood / Q000493:pharmacokinetics; D008175:Lung Neoplasms / Q000097:blood / Q000000981:diagnostic imaging*; D011877:Radionuclide Imaging; D019809:Streptavidin; D014018:Tissue Distribution
chemical_list: D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D007205:Indium Radioisotopes; D001710:Biotin; D019809:Streptavidin