Immunofluorescence demonstration of digoxin in human myocardial tissue and isolated rat cardiac myocytes with monoclonal antibodies.
The aim of the present investigation was to identify the morphological correlates of digoxin binding sites in human heart muscle tissue and isolated viable rat heart myocytes. Cardiac glycoside linked to myocardial cells was demonstrated by monoclonal digoxin specific antibody and by FITC-conjugated anti-mouse immunoglobin serum. This versatile immunofluorescence method can be used in diagnostic and experimental studies of cardiac glycoside binding.
Sótonyi
P
P
Department of Forensic Medicine of Semmelweis University of Medicine, Budapest, Hungary.
Somogyi
E
E
Rajs
J
J
Sundberg
M
M
Andersson
B
B
D000818:Animals; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D002301:Cardiac Glycosides / Q000378:metabolism; D004077:Digoxin / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D009206:Myocardium / Q000737:chemistry*; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D002301:Cardiac Glycosides; D004077:Digoxin