title: Spin-spin relaxation of the phosphodiester resonance in the 31P NMR spectrum of human brain. The determination of the concentrations of phosphodiester components.
abstract: The phosphodiester peak in 31P nuclear magnetic resonance spectra of human brain in vivo is often the most prominent feature of the spectrum. We have demonstrated that this resonance exhibits bi-exponential spin-spin relaxation, giving relaxation times of 2 and 10 ms. We interpret this in terms of the two components which make up the peak, bilayer lipids and the small cytosolic phosphates glycerophosphoethanolamine and glycerophosphocholine. Using the relaxation times and the relative peak heights of the two components we have also been able to quantitate the concentration of the bilayer lipids as 20-40 times that of ATP.
authors.affiliation: Oxford University, Department of Biochemistry, UK.
authors.forename: P M
authors.initials: PM
authors.lastname: Kilby
authors.forename: J L
authors.initials: JL
authors.lastname: Allis
authors.forename: G K
authors.initials: GK
authors.lastname: Radda
mesh_terms: D001923:Brain Chemistry; D005997:Glycerylphosphorylcholine / Q000032:analysis; D006801:Humans; D008051:Lipid Bilayers / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D010714:Phosphatidylethanolamines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*
chemical_list: D008051:Lipid Bilayers; D010714:Phosphatidylethanolamines; D010743:Phospholipids; C002449:glycerophosphoethanolamine; D005997:Glycerylphosphorylcholine