title: Structural features of the McPC603 Fab fragment not defined in the X-ray structure.
abstract: The proteolytic Fab fragment of the well characterized antibody McPC603 was compared to the recombinant Fab fragment, which was obtained in functional form from an Escherichia coli expression system [(1989) Methods Enzymol. 178, 497-515]. We found evidence that the proteolytic fragment is glycosylated at Asn H160 in the CH1 domain, where additional electron density had been observed in the crystal structure [J. Mol. Biol. 190, 593-604]. In addition, its heavy chain is about 30 amino acids longer than visible in the electron density and thus contains the complete hinge region. These structural differences between the recombinant Fab fragment, which had been designed exactly according to the defined electron density, and the proteolytic Fab fragment of McPC603 had no effect on the hapten binding properties of these antigen binding fragments. Yet, it may be important to be aware of these structural features of McPC603 in folding studies and some comparative analyses of antibody structures.
authors.affiliation: Genzentrum der Universität München, Max-Planck-Institut für Biochemie, Martinsried, FRG.
authors.forename: A
authors.initials: A
authors.lastname: Skerra
authors.forename: R
authors.initials: R
authors.lastname: Glockshuber
authors.forename: A
authors.initials: A
authors.lastname: Plückthun
mesh_terms: D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000276:immunology; D002352:Carrier Proteins / Q000737:chemistry*; D004926:Escherichia coli / Q000276:immunology*; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry*; D007136:Immunoglobulins / Q000737:chemistry*; D008969:Molecular Sequence Data; D009194:Myeloma Proteins / Q000737:chemistry*; D011994:Recombinant Proteins / Q000276:immunology; D014961:X-Ray Diffraction
chemical_list: D000907:Antibodies, Bacterial; D002352:Carrier Proteins; D007140:Immunoglobulin Fab Fragments; D007136:Immunoglobulins; D009194:Myeloma Proteins; D011994:Recombinant Proteins; C017551:myeloma immunoglobulins