A radioimmunoassay of bile acids.
A radioimmunoassay for beta-muricholic acid was developed using an antiserum which was prepared by injecting beta-muricholic acid conjugated with bovine serum albumin into rabbits. The antiserum reacted with glyco-beta-muricholic, tauro-beta-muricholic and beta-muricholic acids, but not with other bile acids. The radioimmunoassay showed good reproducibility with inter- and intra-assay coefficients of variations of 6% to 15%. When the validity of the method was examined by comparing it with a gas-liquid chromatography method, a linear correlation was obtained.
Hashimoto
S
S
Shionogi Research Laboratories, Osaka, Japan.
Chikai
T
T
Uchida
K
K
D000818:Animals; D000918:Antibody Specificity; D001647:Bile Acids and Salts / Q000032:analysis*; D002793:Cholic Acids / Q000032:analysis; D002849:Chromatography, Gas; D003429:Cross Reactions; D005260:Female; D006000:Glycocholic Acid / Q000032:analysis; D008297:Male; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D015203:Reproducibility of Results
D001647:Bile Acids and Salts; D002793:Cholic Acids; C004821:muricholic acid; D006000:Glycocholic Acid