tyk2, prototype of a novel class of non-receptor tyrosine kinase genes.
We previously identified a novel protein tyrosine kinase gene, tyk2, by screening a human lymphoid cDNA library with a tyrosine kinase domain specific c-fms restriction fragment under low stringency hybridization conditions. We have now isolated and sequenced a full length tyk2 cDNA clone; demonstrated that this gene is widely expressed in hematopoietic and non-hematopoietic cell lines; and mapped it to chromosome 19p13.2. The cDNA clone is 4176 nucleotides long and codes for a putative protein with a molecular weight of 134 kilodaltons. Hydrophobicity analysis of our sequence does not identify a transmembrane domain, which is found in all members of the receptor class of protein tyrosine kinases; nor can we detect an SH2 domain, found in all previously identified non-receptor protein kinases. We therefore propose that tyk2 is the prototype of a new class of non-receptor protein tyrosine kinases.
Firmbach-Kraft
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I
Department of Pathology, Columbia University, College of Physicians and Surgeons, New York, New York 10032.
Byers
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M
Shows
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Dalla-Favera
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Krolewski
J J
JJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19 / Q000737:chemistry*; D004247:DNA / Q000032:analysis; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
D012333:RNA, Messenger; D004247:DNA; D011505:Protein-Tyrosine Kinases