Antimutagenic action of cobaltous chloride on radiation-induced mutations in cultured Chinese hamster cells.
The effects of cobaltous chloride on 8-azaguanine (8AG)-resistant mutations induced by gamma-rays or ultraviolet (UV) light in cultured Chinese hamster V79 cells were examined. Cobaltous chloride alone had no significant effects on survival and mutations of V79 cells at concentrations less than 1 x 10(-5) M. Cobaltous chloride at a concentration of 3 x 10(-6) M had a marked effect in reducing 8AG-resistant mutations induced by gamma-rays of 2-6 Gy, when cells were incubated for 6-7 days in the presence of cobaltous chloride after gamma-ray irradiation (posttreatment). The pretreatment of cells with cobaltous chloride for 6 days before gamma-ray irradiation reduced 8AG-resistant mutations induced by gamma-rays. Pre- or post-treatment with cobaltous chloride had no such effect on UV-induced mutations, however. The difference in responsiveness to cobaltous chloride between bacterial and mammalian cell systems is discussed.
Yokoiyama
A
A
Department of Induced Mutation, National Institute of Genetics, Shizuoka, Japan.
Kada
T
T
Kuroda
Y
Y
D000818:Animals; D001375:Azaguanine / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003035:Cobalt / Q000494:pharmacology*; D006224:Cricetinae; D003412:Cricetulus; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D008168:Lung; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation; D014466:Ultraviolet Rays
D009153:Mutagens; D003035:Cobalt; C018021:cobaltous chloride; D001375:Azaguanine