title: [MTT assay using fresh surgical specimens with reference to the transfer system and reproducibility in "test center" method].
abstract: A chemosensitivity test (MTT assay) was conducted using 59 fresh surgical specimens collected from Keio University, Kitasato Institute Hospital and 14 affiliated hospitals, in order to assess the specimen transfer system and the reproducibility of the assay results obtained at Keio University and Kitasato Institute Hospital. Although the optical density yielded by the tumor cells in a number of 5 x 10(4)/well and the number of evaluable cases were significantly reduced through the transfer, the chemosensitivity pattern of the specimen was identical before and after the transfer. Fifty seven of 59 cases were evaluable and the concordant rate of the assay results between the two institutes was 80.6% (108/134) among each case-drug combination. Since the transfer system of the specimen was established and the reproducibility of the assay results in two institutes was confirmed, the "test center" method of the MTT assay appears to be possible by collecting the surgical specimens from the affiliated hospitals.
authors.affiliation: Dept. of Surgery, Keio University.
authors.forename: T
authors.initials: T
authors.lastname: Kubota
authors.forename: A
authors.initials: A
authors.lastname: Suto
authors.forename: K
authors.initials: K
authors.lastname: Ishibiki
authors.forename: O
authors.initials: O
authors.lastname: Abe
authors.forename: Y
authors.initials: Y
authors.lastname: Yamada
authors.forename: F
authors.initials: F
authors.lastname: Asanuma
authors.forename: E
authors.initials: E
authors.lastname: Kawamura
authors.forename: T
authors.initials: T
authors.lastname: Yamada
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000970:Antineoplastic Agents / Q000494:pharmacology*; D004396:Coloring Agents; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D005260:Female; D006755:Hospital Shared Services; D006801:Humans; D007564:Japan; D007755:Laboratories, Hospital; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000473:pathology / Q000601:surgery; D015203:Reproducibility of Results; D013048:Specimen Handling; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D000970:Antineoplastic Agents; D004396:Coloring Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue