[Use of plasma clot in the evaluation of fibroblast progenitor cells of human bone marrow].
Cultivation of human bone marrow cells in plasma clot resulted in high efficiency of cloning. It probably results from additional thrombin stimulus on fibroblast-cell proliferation. Another cause of high cloning efficiency is independent of adherence to culture flask surface. Plasma clot system permits clearcut morphological examination. Plasma clot system is in superiority to other culture systems. In this culture system several types of stromal-hemopoietic cells interactions were found, which probably correlate to in vivo interaction of these cells.
Zaritskiĭ
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D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D010949:Plasma; D013234:Stem Cells
Ispol'zovanie plazmennogo sgustka dlia otsenki fibroblastnykh kletok-predshestvennikov kostnogo mozga cheloveka.