Manipulations of cholinesterase gene expression modulate murine megakaryocytopoiesis in vitro.
Megakaryocytopoiesis was selectively inhibited in cultured murine bone marrow cells by a 15-mer oligodeoxynucleotide complementary to the initiator AUG region in butyrylcholinesterase mRNA. Furthermore, conditioned medium from Xenopus oocytes producing recombinant butyrylcholinesterase stimulated megakaryocytopoiesis. These observations implicate butyrylcholinesterase in megakaryocytopoiesis and suggest application of oligodeoxynucleotides for modulating bone marrow development.
Patinkin
D
D
Department of Biological Chemistry, Hebrew University of Jerusalem, Israel.
Seidman
S
S
Eckstein
F
F
Benseler
F
F
Zakut
H
H
Soreq
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D002802:Cholinesterases / Q000235:genetics*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D005796:Genes; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000201:enzymology; D008533:Megakaryocytes / Q000166:cytology* / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D009865:Oocytes / Q000502:physiology; D014982:Xenopus laevis
D015345:Oligonucleotide Probes; D002802:Cholinesterases