title: Air bubble endothelial damage during phacoemulsification in human eye bank eyes: the protective effects of Healon and Viscoat.
abstract: Following insertion of either Healon (sodium hyaluronate) or Viscoat (sodium chondroitin sulfate-sodium hyaluronate) into the anterior chamber of human eye bank eyes, a controlled amount of small air bubbles was introduced into the anterior chamber during phacoemulsification. After vital staining, damage to endothelial cells in the central cornea was quantified. Endothelial damage averaged 4.5% in eyes in which no viscoelastic was used (positive control), whereas damage was only 0.4% (P less than .001) in eyes in which a viscoelastic was inserted but no air bubbles were introduced (negative control). Endothelial damage in test specimens using air plus Healon averaged 4.9%. Damage in test specimens using air plus Viscoat averaged 0.3% (P less than .02). As demonstrated by scanning electron microscopy, many areas in the positive controls and in the Healon test specimens were damaged too extensively to quantitate accurately by the method we used. Thus, Viscoat may prevent or lessen damage to the corneal endothelium by small air bubbles during phacoemulsification.
authors.affiliation: Department of Ophthalmology, University of Utah School of Medicine, Salt Lake City.
authors.forename: M T
authors.initials: MT
authors.lastname: Craig
authors.forename: R J
authors.initials: RJ
authors.lastname: Olson
authors.forename: N
authors.initials: N
authors.lastname: Mamalis
authors.forename: R J
authors.initials: RJ
authors.lastname: Olson
mesh_terms: D000388:Air; D000867:Anterior Chamber; D002387:Cataract Extraction / Q000009:adverse effects*; D002452:Cell Count; D002807:Chondroitin / Q000627:therapeutic use*; D002809:Chondroitin Sulfates; D004338:Drug Combinations; D004728:Endothelium, Corneal / Q000648:ultrastructure*; D005069:Evaluation Studies as Topic; D005125:Eye Banks; D006801:Humans; D006820:Hyaluronic Acid / Q000627:therapeutic use*; D008855:Microscopy, Electron, Scanning; D011897:Random Allocation
chemical_list: D004338:Drug Combinations; C501944:chondroitin sulfate, sodium hyaluronate drug combination; D006820:Hyaluronic Acid; D002807:Chondroitin; D002809:Chondroitin Sulfates