Peroxisome proliferation and modulation of rat liver carcinogenesis by 2,4-dichlorophenoxyacetic acid, 2,4,5-trichlorophenoxyacetic acid, perfluorooctanoic acid and nafenopin.
Using an initiation--selection--promotion protocol for induction of liver tumors in Wistar rats, the modulating action of various peroxisome proliferators on neoplasia as well as on selected biochemical parameters was studied. After treatment with diethylnitrosamine (DEN), the animals were subsequently subjected to a selection procedure involving feeding of 2-acetylaminofluorene (2-AAF), and in the middle of the 2-AAF treatment, a single necrogenic dose of carbon tetrachloride. Following a recovery period, the rats were fed a diet containing 0.1% nafenopin (NAF), 0.015% perfluorooctanoic acid (PFOA), 0.05% 2,4-dichlorophenoxyacetic acid (2,4-D), 0.05% 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) or 0.05% phenobarbital (PB) as a positive control. When the animals were killed, 7 months after initiation, the incidence of hepatocellular carcinoma was 83, 33 and 16% in the animals treated with NAF, PFOA or 2,4,5-T respectively. No cancers were observed in controls, or in the 2,4,-D groups. In comparison with controls, NAF and PFOA caused a 60-and 24-fold increase inthe peroxisomal beta-oxidation of fatty acids respectively, but only about a 2-fold increase in the catalase activity, 2,4-D and/or 2,4,5-T were much less active in this respect, giving approximately a doubling in the rate of fatty acid oxidation. The specific activity of D-amino acid and glycolate oxidases were significantly depressed, whereas the urate oxidase levels were apparently unaffected by the NAF and PFOA treatment. The results suggest that the selective induction of peroxisomal fatty acid oxidation is consistent with the hypothesis that imbalance between H2O2 overproduction and its destruction could play a role in the modulation of hepatocarcinogenesis by peroxisome proliferators.
Abdellatif
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AG
Unité de Biochimie, Toxicologique et Cancérologique, Université Catholique de Louvain, Bruxelles, Belgium.
Préat
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Vamecq
J
J
Nilsson
R
R
Roberfroid
M
M
D015085:2,4,5-Trichlorophenoxyacetic Acid / Q000633:toxicity*; D015084:2,4-Dichlorophenoxyacetic Acid / Q000633:toxicity*; D015073:2-Acetylaminofluorene; D000818:Animals; D002210:Caprylates / Q000633:toxicity*; D002251:Carbon Tetrachloride; D004052:Diethylnitrosamine; D004790:Enzyme Induction / Q000187:drug effects; D005466:Fluorocarbons / Q000633:toxicity*; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced* / Q000473:pathology; D008297:Male; D008830:Microbodies / Q000201:enzymology*; D009255:Nafenopin / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains
D002210:Caprylates; D005466:Fluorocarbons; D009255:Nafenopin; D015084:2,4-Dichlorophenoxyacetic Acid; D004052:Diethylnitrosamine; C023036:perfluorooctanoic acid; D015073:2-Acetylaminofluorene; D015085:2,4,5-Trichlorophenoxyacetic Acid; D002251:Carbon Tetrachloride