[Effect of adenosine on recovery of lowered viability of preserved rat hearts measured by 31P magnetic resonance spectroscopy].
The restoration of the viability of rat hearts preserved for 5 hr by 50 microM adenosine (ADN) was evaluated in terms of the cardiac function after preservation and the recovery of myocardial high energy phosphates measured by 31P magnetic resonance spectroscopy. The hearts were perfused with modified Krebs-Ringer's solution (mKRS) with ADN (ADN group) or without AND (non-ADN group) for 1 hour after 5 hr of preservation by the simple immersion method. Control hearts were perfused but not preserved. ATP and phosphocreatine had decreased to 47.2 +/- 6.6% and 28.0 +/- 8.6% of the base line by 5 hr of preservation, but the levels returned to 147.2 +/- 2.3% and 127.4 +/- 5.6% after 1 hr of perfusion with ADN; at the same time, in the non-ADN group, they were 103.8 +/- 2.3% and 115.8 +/- 8.3% respectively. The same hearts were next perfused for 3 hr with standard Krebs-Ringer's solution so as to beat after the perfusion with mKRS. After 3 hr of beating, LV dP/dt was 3330 +/- 116 mmHg/s in the ADN group, which was not significantly different from the control (3034 +/- 60 mmHg/s). However, in the non-ADN group, the value was 2516 +/- 201 mmHg/s and significantly lower (p less than 0.05) than the control. The rate-pressure product was 19350 +/- 1154 in the ADN group, not significantly different from the control (20899 +/- 709), but it was 14559 +/- 1339 in the non-ADN group, which was significantly lower (p less than 0.01) than the control.(ABSTRACT TRUNCATED AT 250 WORDS)
Iwamoto
K
K
Department of Surgery, Osaka City University Medical School, Japan.
Usui
N
N
Kinoshita
H
H
D000241:Adenosine / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002302:Cardiac Output / Q000187:drug effects; D003080:Cold Temperature; D006321:Heart / Q000502:physiology*; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009926:Organ Preservation / Q000379:methods*; D010761:Phosphorus Radioisotopes; D051381:Rats; D011919:Rats, Inbred Strains; D014022:Tissue Survival / Q000187:drug effects*
D010761:Phosphorus Radioisotopes; D000255:Adenosine Triphosphate; D000241:Adenosine