Isolation and characterization of non-muscle tropomyosin cDNAs of human and mouse origin.
Several cDNA clones of human and mouse non-muscle tropomyosin have been isolated. All the human clones possess a common 23 bp sequence immediate 5' of the initiation codon. However, in the further upstream regions, the nucleotide sequences diverge. Two of the mouse cDNA clones pPSI-8 and pPSI-14 have identical nucleotide sequence in the coding region sequenced. However, 5' of the initiation codon these clones have only 40 identical nucleotides and further upstream the nucleotide sequences diverge. Analysis of the genomic DNAs of mouse cells indicated the possibility of a common gene giving rise to both the tropomyosin cDNAs differing in their 5' ends.
Seth
P
P
NCI-FCRF, Frederick, MD 21701.
Martin-Zanca
D
D
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009857:Oncogenes; D011401:Promoter Regions, Genetic; D012326:RNA Splicing; D015183:Restriction Mapping; D014335:Tropomyosin / Q000235:genetics*
D014335:Tropomyosin; D004247:DNA