Hinf I restriction endonuclease digestion on human fixed metaphase chromosomes.
The authors report on the activity of Hinf I restriction endonuclease on human fixed metaphase chromosomes. Experiments performed by digesting chromosomes just after harvesting or after ageing in methanol-acetic acid displayed a different pattern of digestion on metaphases, since only aged preparations showed gaps on heterochromatic regions of chromosomes 1, 9 and 16 and C-like bands on other chromosomes. In this view, the authors suggest that structural modifications of the DNA, induced by acid fixation, can influence Hinf I activity on fixed metaphase chromosomes.
Stuppia
L
L
Istituto di Citomorfologia, CNR, Chieti, Italy.
Guanciali Franchi
P
P
Calabrese
G
G
Parruti
G
G
Palka
G
G
Bianchi
U
U
D002875:Chromosomes / Q000737:chemistry / Q000187:drug effects*; D004247:DNA / Q000032:analysis / Q000187:drug effects; D015252:Deoxyribonucleases, Type II Site-Specific / Q000494:pharmacology*; D006801:Humans; D008677:Metaphase
D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases