title: [The cytotoxic effect of anti-Ro- (SS-A) antibodies and UVA light on human endothelial cells in vitro].
abstract: There is in photosensitive lupus erythematosus a strong association of high titers of anti-Ro(SS-A) antibodies in the serum of the patient with the development of special skin reactions depending on UV-light. The aim of our experiments was to detect a cytotoxic effect of anti-Ro(SS-A) antibodies and UVA-light on human endothelial cells in vitro (standard trypan blue exclusion test, SEM). After UVA-doses of 1 to 100 J/cm2, which were tested, and influence of serum containing anti-Ro(SS-A) antibodies (62 E, ELISA) membrane destructions of endothelial cells depending on the UVA-dose can be seen, irrespective of the fact whether the antibodies are present throughout the irradiation or added afterwards. This cytotoxic reaction depends on complement. A UVA-dose of 100 J/cm2 causes lethal damage of 40% of the cells. These results were confirmed by scanning electron microscopy.
authors.affiliation: Klinik und Poliklinik für Hautkrankheiten Bereichs Medizin (Charité) der Humboldt-Universität zu Berlin.
authors.forename: B
authors.initials: B
authors.lastname: Lanto
authors.forename: F
authors.initials: F
authors.lastname: Böhm
authors.forename: H
authors.initials: H
authors.lastname: Meffert
authors.forename: N
authors.initials: N
authors.lastname: Sönnichsen
mesh_terms: D000974:Antibodies, Antinuclear / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D002470:Cell Survival / Q000528:radiation effects*; D003470:Culture Media; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D008854:Microscopy, Electron; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D014466:Ultraviolet Rays; D000099207:SS-A Antigen
chemical_list: D000974:Antibodies, Antinuclear; D001324:Autoantigens; D003470:Culture Media; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C116006:RO60 protein, human; C035356:SS-A antibodies
vernacular_title: Zytotoxische Wirkung von Anti-Ro- (SS-A-) Antikörpern und UVA-Licht auf menschliche Endothelzellen in vitro.