title: Modified oligopeptides designed to interact with the HIV-1 proteinase inhibit viral replication.
abstract: The human immunodeficiency virus 1 (HIV-1) codes for a proteinase that cuts viral proteins at specific sites. We have tested 13 modified oligopeptides related to these cleavage sites to see if they inhibit viral replication. To indicate whether a decrease in replication could be due to a general inhibition of cell metabolism, we also measured the effect of the peptides on cellular protein synthesis. Three of the peptides tested (Ac-Gln-Asn-Sta-Val-NH2, Ac-Gln-Asn-Sta-Val-Val-NH2, and Ac-Glu-Asn-Sta-Ile-NH2) inhibited HIV-1 replication at concentrations that did not inhibit protein synthesis. Ac-Gln-Asn-Sta-Val-NH2 was the most potent, causing an approximately 40% decrease in viral replication, measured as the synthesis of HIV-1 antigens and the formation of infectious particles.
authors.affiliation: Department of Virology, National Institute of Public Health, Oslo, Norway.
authors.forename: B
authors.initials: B
authors.lastname: Grinde
authors.forename: O
authors.initials: O
authors.lastname: Hungnes
authors.forename: E
authors.initials: E
authors.lastname: Tjøtta
mesh_terms: D000595:Amino Acid Sequence; D002478:Cells, Cultured; D016333:HIV Protease / Q000378:metabolism*; D015497:HIV-1 / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*; D015215:Zidovudine / Q000494:pharmacology
chemical_list: D009842:Oligopeptides; D011480:Protease Inhibitors; D015215:Zidovudine; D016333:HIV Protease