title: Influence of bestatin on production of granulocyte-macrophage colony-stimulating factor from human peripheral blood mononuclear cells in vitro.
abstract: Bestatin, (25, 3R)-3-amino-2-hydroxy-4-phenylbutyryl-L-leucine, enhanced proliferation of normal human bone marrow granulocyte-macrophage progenitor cells to form CFU-GM colonies in viscous methylcellulose medium in vitro. To elucidate the mechanisms of this effect, the levels of colony-stimulating factor (CSF) in culture supernatant of 1 x 10(6)/ml peripheral blood mononuclear cells (PBMC) cocultured with various concentrations of bestatin were determined using our newly developed sandwich enzyme-linked immunosorbent assay (ELISA). The level of granulocyte/macrophage-CSF (GM-CSF) in the supernatant in the presence of 0.1 microgram/ml bestatin was 312 pg/ml by this ELISA. The production of GM-CSF increased with increasing amounts of added bestatin.
authors.affiliation: First Department of Internal Medicine, Faculty of Medicine, Kyushu University Hospital, Fukuoka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Okamura
authors.forename: F
authors.initials: F
authors.lastname: Omori
authors.forename: K
authors.initials: K
authors.lastname: Haga
authors.forename: H
authors.initials: H
authors.lastname: Baba
authors.forename: K
authors.initials: K
authors.lastname: Sugimachi
authors.forename: Y
authors.initials: Y
authors.lastname: Niho
mesh_terms: D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D006801:Humans; D007930:Leucine / Q000031:analogs & derivatives* / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism
chemical_list: D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D007930:Leucine; C012211:ubenimex