[Genotoxicity of a standardized Hypericum extract].
St. John's wort (Hypercum perforatum) contains hypericin and hypericin-like substances as well as flavonoids, of which particularly Quercetin has generated a wide-spread controversial discussion with respect to mutagenic action. The genotoxicity of a standardized aqueous ethanolic Hypericum extract (Hypericum extract Steigerwald, Psychotonin M) was verified in different in-vivo and in-vitro testsystems with mammalian cells. The in-vitro investigations were performed with the HGPRT (hypoxanthine guanidine phosphoribosyl transferase)-test, UDS (unscheduled DNA synthesis)-test and with the cell transformation test using Syrian hamster embryo cells. Both the in-vitro tests as well as the in-vivo tests--fur spot test of the mouse and the chromosome aberration test with the bone marrow cells of the chinese hamster--were negative, giving completely no indication of a mutagenic potential of Hypericum extract. These investigations lend support to the view that results from bacterial short-term tests are of very limited transferability to human.
Okpanyi
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Steigerwald Arzneimittelwerk GmbH, Darmstadt.
Lidzba
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Scholl
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Miltenburger
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HG
D000818:Animals; D000873:Anthracenes; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000096:biosynthesis; D005260:Female; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008213:Lymphocyte Activation; D008647:Mesocricetus; D051379:Mice; D009152:Mutagenicity Tests; D009153:Mutagens; D010569:Perylene / Q000031:analogs & derivatives / Q000633:toxicity; D010936:Plant Extracts / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains
D000873:Anthracenes; D009153:Mutagens; D010936:Plant Extracts; D010569:Perylene; C004965:hypericin; D004247:DNA; D007041:Hypoxanthine Phosphoribosyltransferase
Genotoxizität eines standardisierten Hypericum-Extraktes.