In vitro 31P NMR spectroscopy of the reproductive organs of the male rat: an improved preparation method.
In vitro 31P spectroscopy can be applied to disentangle the broad resonances in in vivo 31P NMR spectra of the reproductive organs of the male rat. However, adenosinetriphosphate (ATP) and glycerophosphocholine (GPC), present in large amounts in vivo, are nearly completely absent in vitro. With a modified PCA extraction method, in which the organs were lyophilized before extraction, a large amount of ATP and GPC could be found in vitro. This new extraction method is probably not only restricted to in vitro analysis of the testis but is likely to be useful for analysis of other organs such as kidney and liver.
van der Grond
J
J
Department of Radiodiagnosis, University Hospital Utrecht, The Netherlands.
Seijkens
D
D
Mali
W P
WP
D000818:Animals; D005612:Freeze Drying; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000033:anatomy & histology* / Q000737:chemistry