title: tyk2, prototype of a novel class of non-receptor tyrosine kinase genes.
abstract: We previously identified a novel protein tyrosine kinase gene, tyk2, by screening a human lymphoid cDNA library with a tyrosine kinase domain specific c-fms restriction fragment under low stringency hybridization conditions. We have now isolated and sequenced a full length tyk2 cDNA clone; demonstrated that this gene is widely expressed in hematopoietic and non-hematopoietic cell lines; and mapped it to chromosome 19p13.2. The cDNA clone is 4176 nucleotides long and codes for a putative protein with a molecular weight of 134 kilodaltons. Hydrophobicity analysis of our sequence does not identify a transmembrane domain, which is found in all members of the receptor class of protein tyrosine kinases; nor can we detect an SH2 domain, found in all previously identified non-receptor protein kinases. We therefore propose that tyk2 is the prototype of a new class of non-receptor protein tyrosine kinases.
authors.affiliation: Department of Pathology, Columbia University, College of Physicians and Surgeons, New York, New York 10032.
authors.forename: I
authors.initials: I
authors.lastname: Firmbach-Kraft
authors.forename: M
authors.initials: M
authors.lastname: Byers
authors.forename: T
authors.initials: T
authors.lastname: Shows
authors.forename: R
authors.initials: R
authors.lastname: Dalla-Favera
authors.forename: J J
authors.initials: JJ
authors.lastname: Krolewski
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19 / Q000737:chemistry*; D004247:DNA / Q000032:analysis; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D012333:RNA, Messenger; D004247:DNA; D011505:Protein-Tyrosine Kinases