Partial hepatectomy and mediators of inflammation decrease the expression of liver alpha 2-HS glycoprotein gene in rats.
Liver mRNA levels of two acute phase reactant (APR) proteins, alpha 2-HS glycoprotein (a major negative APR) and alpha 1-acid glycoprotein (a major positive APR) were measured in male rats at different times after the administration of turpentine, of tumor necrosis factor, or following partial hepatectomy. In every case, a marked decrease in mRNA levels of alpha 2-HS glycoprotein was observed which reached a maximum at 24 h. A concomitant increase of alpha 1-acid glycoprotein mRNA levels was observed under the same conditions. These results indicate that the decreased levels of alpha 2-HS glycoprotein induced by the acute-phase response following inflammatory mediators and partial hepatectomy are due to a down-regulation of the gene expression of this protein in rat liver.
Daveau
M
M
INSERM Unité 78, BP 73, 76233 Bois-Guillaume, France.
Davrinche
C
C
Djelassi
N
N
Lemetayer
J
J
Julen
N
N
Hiron
M
M
Arnaud
P
P
Lebreton
J P
JP
D000818:Animals; D015152:Blotting, Northern; D005353:Fibronectins / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D005796:Genes / Q000187:drug effects; D006498:Hepatectomy; D007249:Inflammation; D008297:Male; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D014425:Turpentine
D005353:Fibronectins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D014425:Turpentine