Recombinant DNA probe detecting Eperythrozoon suis in swine blood.
A genomic library to Eperythrozoon suis DNA was constructed in lambda gt11, and from this library, E suis clone KSU-2 was identified as a potential diagnostic probe. In hybridization experiments that used 100-microliters samples of blood collected in chaotropic salt solutions, the KSU-2 probe hybridized strongly with purified E suis organisms and blood samples from splenectomized swine that were parasitized with E suis. However, the probe under stringent conditions did not give radiographic indications of hybridizing with equine blood DNA, bovine blood DNA infected with Anaplasma marginale, canine blood DNA infected with Ehrlichia canis, feline blood DNA infected with Haemobartonella felis, or uninfected swine blood DNA.
Oberst
R D
RD
Department of Pathology, College of Veterinary Medicine, Kansas State University, Manhattan 66506.
Hall
S M
SM
Jasso
R A
RA
Arndt
T
T
Wen
L
L
D000818:Animals; D001345:Autoradiography / Q000662:veterinary; D015342:DNA Probes; D004269:DNA, Bacterial / Q000097:blood* / Q000235:genetics; D004274:DNA, Recombinant; D015723:Gene Library; D006386:Hemagglutination Tests / Q000662:veterinary; D009174:Mycoplasma / Q000235:genetics* / Q000302:isolation & purification; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000662:veterinary*; D009693:Nucleic Acid Hybridization / Q000235:genetics; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000175:diagnosis* / Q000235:genetics
D015342:DNA Probes; D004269:DNA, Bacterial; D004274:DNA, Recombinant