Cloning of the determinants for microcin D93 production and analysis of three different D-type microcin plasmids.
Three different microcin plasmids coding for D-type microcins were analyzed. Two of the plasmids (pMccD93 and pCP101) were small, multicopy plasmids and were closely related. The third plasmid (pCP106) was a conjugative, antibiotic multiresistance plasmid. Although plasmids pCP101 and pCP106 were previously classified as A-type microcin plasmids, we have determined that they are, in fact, D type. Furthermore, the determinants for microcin D93 production were cloned from plasmid pMccD93, and a DNA probe for the region implicated in the synthesis of microcin was obtained. This probe hybridized to plasmid C from Escherichia coli strain V517, indicating that this plasmid might be involved in the synthesis of a D-type microcin. The characteristics of replication of plasmid pCP106 were analyzed and appeared to be similar to those of ColEl plasmids, although pCP106 is a conjugative single-copy plasmid.
Martínez
J L
JL
Servicio de Microbiología, Hospital Ramón y Cajal, Madrid, Spain.
Pérez-Díaz
J C
JC
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000494:pharmacology; D001430:Bacteriocins / Q000096:biosynthesis*; D003001:Cloning, Molecular / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005838:Genotype; D008826:Microbial Sensitivity Tests; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D015183:Restriction Mapping
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D004269:DNA, Bacterial; C020411:microcin