[A method for producing peptide maps of picomolar quantities of protein labelled with the Bolton-Hunter reagent in polyacrylamide gel].
A method of radioiodination to high specific activity by Bolton Hunter reagent of fixed and stained histones in polyacrylamide gel is described. The comparative peptide maps of the histones H1 and H3, labelled in solution and the gel are presented.
Kolomiĭtseva
G Ia
GIa
Kurochkina
L P
LP
Nikitina
I V
IV
Moiseeva
T I
TI
D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods; D006657:Histones / Q000737:chemistry; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D010449:Peptide Mapping / Q000295:instrumentation / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D013388:Succinimides
D006657:Histones; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D011506:Proteins; D013388:Succinimides; C022581:Bolton-Hunter reagent
Metod polucheniia peptidnykh kart pikomoliarnykh kolichestv belka, mechennogo reagentom Boltona--Khantera v poliakrilamidnom gele.