Metabolic activation of quercetin mutagenicity.
The mutagenicity of quercetin was reinvestigated using the Salmonella/microsome test. The mutagenicity of quercetin was enhanced by the cytosolic fraction of liver extract (S100), or by ascorbate, and even more by the complete liver supernatant (S9) in the presence of cofactors (NADP and glucose-6-phosphate). The formation of metabolites by the S9 enzymes was demonstrated by reverse-phase HPLC.
Vrijsen
R
R
Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Belgium.
Michotte
Y
Y
Boeyé
A
A
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D001711:Biotransformation; D002457:Cell Extracts; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000502:physiology; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D009153:Mutagens / Q000378:metabolism*; D009249:NADP / Q000502:physiology; D011794:Quercetin / Q000378:metabolism* / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D012475:Salmonella / Q000235:genetics; D013347:Subcellular Fractions / Q000378:metabolism
D002457:Cell Extracts; D005958:Glucosephosphates; D009153:Mutagens; D009249:NADP; D019298:Glucose-6-Phosphate; D011794:Quercetin; D001205:Ascorbic Acid