title: P190 BCR/ABL transcript in a case of Philadelphia-positive multiple myeloma.
abstract: Philadelphia positive multiple myeloma is a very rare event and, so far, no molecular data about the involvement of the BCR and C-ABL genes are available. We report here the case of a 64-year-old woman presenting with a typical multiple myeloma and a complex Philadelphia (Ph) chromosome that we investigated at a molecular level using conventional DNA techniques and the polymerase chain reaction (PCR). No rearrangement was observed within the major breakpoint cluster region (M-BCR) although she was found to have a P190 BCR/ABL hybrid transcript using PCR. As far as we know, this is the first description of a P190-type mRNA in a patient with a chronic lymphoid disorder. Since P190 is almost always associated in man with acute forms of hematological malignancies, this suggests that other factors may play a role in determining the phenotype of the disease.
authors.affiliation: Department of Haematology, University of Louvain Medical School, Brussels, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Martiat
authors.forename: C
authors.initials: C
authors.lastname: Mecucci
authors.forename: Y
authors.initials: Y
authors.lastname: Nizet
authors.forename: M
authors.initials: M
authors.lastname: Stul
authors.forename: M
authors.initials: M
authors.lastname: Philippe
authors.forename: J J
authors.initials: JJ
authors.lastname: Cassiman
authors.forename: J L
authors.initials: JL
authors.lastname: Michaux
authors.forename: H
authors.initials: H
authors.lastname: Van den Berghe
authors.forename: G
authors.initials: G
authors.lastname: Sokal
mesh_terms: D001483:Base Sequence; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D008875:Middle Aged; D008969:Molecular Sequence Data; D009101:Multiple Myeloma / Q000235:genetics / Q000378:metabolism*; D015345:Oligonucleotide Probes; D010677:Philadelphia Chromosome; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D004273:DNA, Neoplasm; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D016044:Fusion Proteins, bcr-abl