title: Thrombin stimulates the production of immunoreactive endothelin-1 in cultured human umbilical vein endothelial cells.
abstract: The present study was designed to examine the effect of thrombin on endothelin production by cultured human umbilical vein endothelial cells (HUVEC) by radioimmunoassay. Cultured HUVEC released immunoreactive endothelin-1 (ir-endothelin-1) into the medium in a time-dependent manner. This release was inhibited completely by 10 micrograms/mL cycloheximide and is, therefore, directly related to de novo protein synthesis. Further endothelin release was stimulated by the addition of thrombin to the culture medium. Thrombin-induced ir-endothelin-1 release also was inhibited completely by 10 micrograms/mL cycloheximide. These results suggest that HUVEC in culture produce ir-endothelin-1 slowly, but continuously, and that this production is stimulated by thrombin.
authors.affiliation: First Department of Internal Medicine, Osaka City University Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kohno
authors.forename: K
authors.initials: K
authors.lastname: Yasunari
authors.forename: K
authors.initials: K
authors.lastname: Yokokawa
authors.forename: K
authors.initials: K
authors.lastname: Murakawa
authors.forename: T
authors.initials: T
authors.lastname: Horio
authors.forename: Y
authors.initials: Y
authors.lastname: Kanayama
authors.forename: M
authors.initials: M
authors.lastname: Fuzisawa
authors.forename: T
authors.initials: T
authors.lastname: Inoue
authors.forename: T
authors.initials: T
authors.lastname: Takeda
mesh_terms: D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D016232:Endothelins / Q000096:biosynthesis* / Q000276:immunology; D004730:Endothelium, Vascular / Q000378:metabolism*; D006801:Humans; D013917:Thrombin / Q000494:pharmacology*; D014471:Umbilical Veins / Q000378:metabolism; D014662:Vasoconstrictor Agents / Q000378:metabolism*
chemical_list: D016232:Endothelins; D014662:Vasoconstrictor Agents; D003513:Cycloheximide; D013917:Thrombin