Biosynthesis of a membrane adhesion zone fraction throughout the cell cycle of Escherichia coli.
Synchronized cells of Escherichia coli were pulse-labeled with [3H]leucine and subjected to membrane fractionation to determine whether a fraction that is enriched for membrane-murein adhesion zones (fraction OML) was preferentially generated at specific times during the cell cycle, as previously suggested from studies of lkyD and cha mutants. Contrary to this prediction, the experiments showed that OML was formed continuously during the division cycle.
Joseleau-Petit
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D
Institut Jacques Monod, Université Paris VII, France.
Kepes
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F
Peutat
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D'Ari
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R
Rothfield
L I
LI
D001422:Bacterial Adhesion; D002250:Carbon Radioisotopes; D002453:Cell Cycle; D002462:Cell Membrane / Q000502:physiology; D004926:Escherichia coli / Q000166:cytology / Q000502:physiology*; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D010457:Peptidoglycan / Q000096:biosynthesis*; D014316:Tritium
D002250:Carbon Radioisotopes; D010457:Peptidoglycan; D014316:Tritium; D007930:Leucine