title: Mutational analysis of enhancer domains responsive to trans-activation by the X gene of human hepatitis B virus.
abstract: The X gene product of human hepatitis B virus (HBV) trans-activates the HBV enhancer. In order to identify domains responsive to trans-activation by the X gene, we introduced a series of mutations into the HBV enhancer and assayed the enhancer activities in the presence of the X gene product. Our results suggest that the EP domain of the enhancer is essential for trans-activation by the X gene.
authors.affiliation: Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, Springfield.
authors.forename: G
authors.initials: G
authors.lastname: Goodarzi
authors.forename: H
authors.initials: H
authors.lastname: Ohno
authors.forename: R
authors.initials: R
authors.lastname: Adams
authors.forename: A
authors.initials: A
authors.lastname: Darabi
authors.forename: A
authors.initials: A
authors.lastname: Tewari
authors.forename: M
authors.initials: M
authors.lastname: Watabe
authors.forename: K
authors.initials: K
authors.lastname: Watabe
mesh_terms: D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003001:Cloning, Molecular; D004279:DNA, Viral; D004742:Enhancer Elements, Genetic; D004926:Escherichia coli / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016366:Open Reading Frames; D015534:Trans-Activators / Q000502:physiology*; D014407:Tumor Cells, Cultured; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D004279:DNA, Viral; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; D015500:Chloramphenicol O-Acetyltransferase