Evidence for the role of 34-kDa galactoside-binding lectin in transformation and metastasis.
The endogenous Mr 34,000 galactoside-binding lectin (L-34) is found at elevated levels in a wide variety of neoplastic cells and correlative evidence suggests that it is involved in tumor metastasis in vivo and in transformation in vitro. We demonstrate here that introduction of recombinant L-34 into tumorigenic, weakly metastatic UV-2237-cl-15 fibrosarcoma cells results in an increased incidence of experimental lung metastases in syngeneic and nude mice. Transfection of normal BALB/c-A31 cloned fibroblasts with functional L-34 results in acquisition of anchorage-independent growth and in morphological transformation in vitro but not in tumorigenicity in vivo. These results provide direct evidence that the cellular expression of L-34 is associated with some aspects of transformation and with metastasis, but not with tumorigenicity per se.
Raz
A
A
Cancer Metastasis Program, Michigan Cancer Foundation, Detroit 48201.
Zhu
D G
DG
Hogan
V
V
Shah
N
N
Raz
T
T
Karkash
R
R
Pazerini
G
G
Carmi
P
P
D000818:Animals; D002455:Cell Division / Q000502:physiology; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D004247:DNA / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D005354:Fibrosarcoma / Q000235:genetics / Q000473:pathology; D037161:Galectins; D006388:Hemagglutinins / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D051379:Mice; D009362:Neoplasm Metastasis / Q000235:genetics / Q000503:physiopathology*; D011994:Recombinant Proteins / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured
D037161:Galectins; D006388:Hemagglutinins; D011994:Recombinant Proteins; D004247:DNA