Conversion of rat urinary prokallikrein to its active form.
A peptide derived from rat urinary prokallikrein by trypsin treatment comprised 7 amino acids, the sequence (Ala-Pro-Pro-Val-Gln-Ser-Arg) of which was identical with that of the N-terminal region in prokallikrein. Thus, with trypsin treatment, rat urinary prokallikrein is converted to the active form with the release of the N-terminal propeptide consisting of 7 amino acids. An Arg-1-Val+1 bond in the prokallikrein was found to be the site of proteolytic cleavage of the propeptide.
Takenobu
Y
Y
Department of Pharmacology, Osaka University of Pharmaceutical Sciences, Japan.
Takaoka
M
M
Suyama
E
E
Yano
M
M
Morimoto
S
S
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004792:Enzyme Precursors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D007610:Kallikreins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D051381:Rats; D014357:Trypsin / Q000378:metabolism
D004792:Enzyme Precursors; D007610:Kallikreins; C052910:prokallikrein, tissue; D014357:Trypsin