Renal epithelial amino acid concentrations in mercury-induced and postischemic acute renal failure.
The concentration of 18 alpha-amino acids (AAs) in plasma and renal cortical cell water were measured 3 or 24 hr after 1 hr of unilateral renal artery clamping or 24 or 48 hr after 15 mg/kg body weight HgCl2 injection sc as a test of epithelial integrity. Cellular glycine (Gly), hydroxyproline (Hpr), ornithine (Orn), phenylalanine (Phe), serine (Ser), and tryptophan (Trp) concentrations were depressed 24 hr after HgCl2 (p less than 0.05), but the remaining 12 AAs were not distinguishable from control despite the presence of severe renal failure. ARginine (Arg), glutamic acid (Glu), and valine (Val) also were decreased (P less than 0.05) 24 hr later, but concentrations of half of all measured AAs were still normal. Cellular alanine (Ala), Arg, Glu, Gly, Phe, and Ser concentrations were decreased 3 hr after ischemia, p less than 0.05, but 12 AAs were unchanged and only Arg, Phe, Ser, and threonine (Thr) were reduced 24 hr after ischemia was reversed. Concentrations of even the most affected AAs remained notably higher than in plasma in both forms of acute renal failure (ARF). Total loss of AAs from a small proportion of tubular cells would be hidden by essentially normal concentrations in the rest, and such losses may well have occurred. Unless cellular AAs in ARF are almost completely bound, however, the well-maintained cell:plasma AA concentration ratios indicate that cellular energetics were adequate for AA uptake and that epithelial permeability to AAs in the vast majority of cells was not greatly disturbed. Such findings suggest that most of the epithelium, although seriously damaged, had remained viable.
Duran
M A
MA
Department of Medicine, Medical College of Virginia, Richmond.
Spencer
D
D
Weise
M
M
Kronfol
N O
NO
Spencer
R F
RF
Oken
D E
DE
D058186:Acute Kidney Injury / Q000139:chemically induced / Q000378:metabolism*; D000596:Amino Acids / Q000097:blood / Q000378:metabolism*; D000818:Animals; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D005260:Female; D007511:Ischemia / Q000378:metabolism*; D007672:Kidney Cortex / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism*; D008627:Mercuric Chloride / Q000633:toxicity*; D008630:Mercury Poisoning / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
D000596:Amino Acids; D008627:Mercuric Chloride