title: Radioimmunoassay for albuterol using a monoclonal antibody: application for direct quantification in horse urine.
abstract: A monoclonal antibody was synthesized in mouse against the O-(3-carboxypropionyl) derivative of albuterol linked to bovine serum albumin. Isotyping of this material revealed the IgG1 class characterized by an affinity constant of 1.03 nM-1 and a density of sites of 0.55 nM. This antibody was found specific as its cross-reactivity to structurally related molecules was less than 1% except for clenbuterol (75%). A radioimmunoassay was set up with culture supernatant (final dilution 1/1000) and [3H] albuterol. The calibration curve was characterized by a maximum binding of 28%, an ED50 of 1.15 pmol per tube, the detection limit was 28.8 fmol/tube and the linearity of the response was up to 39.8 pmol/tube. This RIA method has been used for direct quantitation of albuterol in horse urine without any clean-up or extraction step.
authors.affiliation: Faculté de pharmacie, Université de Montréal, Québec, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Adam
authors.forename: H
authors.initials: H
authors.lastname: Ong
authors.forename: D
authors.initials: D
authors.lastname: Sondag
authors.forename: A
authors.initials: A
authors.lastname: Rapaille
authors.forename: S
authors.initials: S
authors.lastname: Marleau
authors.forename: M
authors.initials: M
authors.lastname: Bellemare
authors.forename: P
authors.initials: P
authors.lastname: Raymond
authors.forename: D
authors.initials: D
authors.lastname: Giroux
authors.forename: J K
authors.initials: JK
authors.lastname: Loo
authors.forename: N
authors.initials: N
authors.lastname: Beaulieu
mesh_terms: D000420:Albuterol / Q000652:urine*; D000818:Animals; D000911:Antibodies, Monoclonal; D000915:Antibody Affinity; D000918:Antibody Specificity; D005260:Female; D006736:Horses / Q000652:urine*; D007132:Immunoglobulin Isotypes; D051379:Mice; D011863:Radioimmunoassay; D015203:Reproducibility of Results
chemical_list: D000911:Antibodies, Monoclonal; D007132:Immunoglobulin Isotypes; D000420:Albuterol