title: Antibodies to morphine in workers exposed to opiates at a narcotics manufacturing facility and evidence for similar antibodies in heroin abusers.
abstract: According to the International Narcotics Control Board, over 45,000 kg of morphine and 54,000 kg of codeine were ethically manufactured in 1986 at three facilities in the United States. Little information exists about possible adverse health effects associated with workplace exposure to opiate compounds in this industry. Because there are no specific federal standards for workplace exposure to narcotic dusts, exposure-control defaults to the nuisance dust standard (10 mg/m3, as an 8 hr time-weighted average). Narcotics manufacturing workers were evaluated for anti-morphine IgG before and 10 mo. after the implementation of an improved respiratory protection program (RPP). Significantly elevated IgG levels were measured before the improved RPP (P less than 0.005). After the improved RPP, a significant reduction was observed (P less than 0.001), suggesting that specific antibody levels could be used as biomarkers of exposure. Inhibition studies showed that the antibodies were specifically directed against morphine with some cross reactivity with morphine derivatives. Preliminary results are also shown which indicate that similar anti-morphine antibodies are present in the sera of intravenous heroin abusers. Elevated levels (P less than 0.05) of anti-morphine antibodies were detected in sera from heroin abusers, providing evidence that similar antibodies may be produced from non-occupational exposure to opiates. These finding have potentially far-reaching implications for addiction research and drug testing.
authors.affiliation: Department of Health and Human Services, Public Health Service, Centers for Disease Control, Cincinnati, OH.
authors.forename: R E
authors.initials: RE
authors.lastname: Biagini
authors.forename: S L
authors.initials: SL
authors.lastname: Klincewicz
authors.forename: G M
authors.initials: GM
authors.lastname: Henningsen
authors.forename: B A
authors.initials: BA
authors.lastname: MacKenzie
authors.forename: J S
authors.initials: JS
authors.lastname: Gallagher
authors.forename: D I
authors.initials: DI
authors.lastname: Bernstein
authors.forename: I L
authors.initials: IL
authors.lastname: Bernstein
mesh_terms: D000397:Air Pollution / Q000517:prevention & control; D000918:Antibody Specificity; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006556:Heroin Dependence / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D009020:Morphine / Q000737:chemistry / Q000276:immunology*; D009294:Narcotics / Q000138:chemical synthesis*; D016273:Occupational Exposure / Q000517:prevention & control; D012907:Smoking; D013329:Structure-Activity Relationship
chemical_list: D007074:Immunoglobulin G; D009294:Narcotics; D009020:Morphine