Newly identified type of beta actin reduces invasiveness of mouse B16-melanoma.
Low metastatic parent B16 melanoma and isolated B16-F1 cell lines have a third actin designated as beta m(Ax:previously). beta m actin is scantily or not at all detected in highly metastatic cell lines, such as B16-F10 and BL6. To directly assess the physiological role of beta m in phenotypic changes of B16 melanoma, we transfected expression plasmids of beta m into B16-F10 cells. The actin expressed in the transfectants is located largely in cytoskeletal fractions. The transfectants exhibited a larger number of stress fibers and a lower invasiveness than did the recipient cells. Thus, beta m actin plays an important role in the organization of actin stress fibers, the result being a decrease in invasiveness of B16 melanoma.
Sadano
H
H
Department of Experimental Cell Research, Kyushu University, Fukuoka, Japan.
Taniguchi
S
S
Baba
T
T
D000199:Actins / Q000235:genetics / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D004247:DNA; D008545:Melanoma / Q000235:genetics* / Q000473:pathology / Q000556:secondary; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D014162:Transfection; D014407:Tumor Cells, Cultured
D000199:Actins; D004247:DNA