title: Unequal distribution of membrane components between pseudopodia and cell bodies of platelets.
abstract: Platelet pseudopodia were compared to platelet cell bodies with respect to their lipid composition, fatty acid distribution and protein composition. The methodology for producing pseudopodial preparations of platelets stimulated with thrombin, ADP or calcium ionophore was established. The separation of pseudopodia and cell bodies was verified by electron microscopic examination of the respective platelet components. Lipid analyses demonstrated a preponderance of lysophospholipids and sphingomyelin in pseudopodial preparations and a large increase in mono-, di- and tri-ene fatty acids as compared to cell bodies. Changes were also evident in the protein composition evaluated by one- and two-dimensional SDS-polyacrylamide gel electrophoresis and by [32P]ATP labeling of exofacial membrane proteins. A protein of approximately 68 kDa which reacted strongly with antibody to PlA1, was prominantly displayed in platelet pseudopodia. Thus, our studies demonstrate a heterogeneous distribution of lipids and proteins in a mammalian membrane system which may have important implications for the functional behavior of the cell.
authors.affiliation: Division of Hematology/Oncology, Memorial Hospital of Rhode Island, Pawtucket 02860.
authors.forename: J
authors.initials: J
authors.lastname: Jandak
authors.forename: X L
authors.initials: XL
authors.lastname: Li
authors.forename: N
authors.initials: N
authors.lastname: Kessimian
authors.forename: M
authors.initials: M
authors.lastname: Steiner
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism* / Q000648:ultrastructure; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000097:blood*; D006801:Humans; D008246:Lysophospholipids / Q000097:blood; D010743:Phospholipids / Q000097:blood*; D011554:Pseudopodia / Q000378:metabolism* / Q000648:ultrastructure; D012701:Serotonin / Q000097:blood; D013109:Sphingomyelins / Q000097:blood; D013917:Thrombin / Q000494:pharmacology
chemical_list: D002121:Calcium Channel Blockers; D005227:Fatty Acids; D008246:Lysophospholipids; D010743:Phospholipids; D013109:Sphingomyelins; D012701:Serotonin; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D013917:Thrombin