title: Evidence for surface-coat localisation of a monoclonal antibody-isolated merozoite antigen of Babesia divergens.
abstract: The localisation of monoclonal antibody-derived merozoite antigens of Babesia divergens was examined using immunogold electron microscopy and immunoprecipitation of the monoclonal antibody with both biosynthetically and surface-labelled parasites. Immunogold labelling provided evidence that the antigens are components of the surface coat of the merozoite. Immunoprecipitation with biosynthetically labelled parasites showed the antigens to be of parasite origin, whereas surface labelling confirmed that the antigens form part of the surface coat.
authors.affiliation: Department of Biology, Imperial College of Science, Technology and Medicine, London, UK.
authors.forename: C M
authors.initials: CM
authors.lastname: Winger
authors.forename: E U
authors.initials: EU
authors.lastname: Canning
authors.forename: R H
authors.initials: RH
authors.lastname: Hartley
authors.forename: A
authors.initials: A
authors.lastname: Gunn
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000953:Antigens, Protozoan / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis; D001403:Babesia / Q000276:immunology* / Q000648:ultrastructure; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D011233:Precipitin Tests
chemical_list: D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000954:Antigens, Surface