Specific interaction of a partially purified Xenopus transcription factor IIIC (TFIIIC) with frog tRNA gene.
Transcription factor IIIC (TFIIIC) from Xenopus has been partially purified and characterized. Footprinting analyses indicate that a partially purified TFIIIC fraction contains an activity which specifically recognizes the "B" block element of tRNA gene. In addition, two other regions located downstream from the "B" block sequence are also protected. Protection experiments on 5S genes by DNAase I with either TFIIIC alone or TFIIIA and TFIIIC produced a minimal change in the cleavage pattern implying that TFIIIC does not intimately associate with DNA. The implications of these findings in relation to the class III gene transcription are discussed.
Zhang
Z G
ZG
Eye Research Institute of Oakland University, Rochester, Michigan 48309.
Shastry
B S
BS
D000818:Animals; D004247:DNA / Q000235:genetics / Q000378:metabolism; D003850:Deoxyribonuclease I; D005260:Female; D005796:Genes; D010053:Ovary / Q000378:metabolism; D010957:Plasmids; D012343:RNA, Transfer / Q000235:genetics*; D014157:Transcription Factors / Q000302:isolation & purification / Q000378:metabolism*; D019474:Transcription Factors, TFIII; D014158:Transcription, Genetic; D014981:Xenopus; D000098832:Transcription Factor TFIIIC
D014157:Transcription Factors; D019474:Transcription Factors, TFIII; D000098832:Transcription Factor TFIIIC; D004247:DNA; D012343:RNA, Transfer; D003850:Deoxyribonuclease I