The technicon H6000 analyzer discriminates chronic lymphocytic leukemia from other B-cell leukemias through automatic assessment of large unstained cells.
The separation of chronic lymphocytic leukemia of B-cell origin from other chronic B-cell leukemias is subjective, being largely based on the morphologic features of the lymphoid cells in the peripheral blood. The percentage of large unstained cells determined with a Technicon H6000 analyzer (an automated blood cell differential analyzer, Technicon Instruments Corp, Tarrytown, NY) was used as a cell volume variable in an investigation of 70 cases of chronic lymphocytic leukemia of B-cell origin and of other chronic B-cell leukemias. The significant degree of correlation between the percentage of large unstained cells and morphoimmunophenotypic diagnosis, although obtained for a relatively small number of cases, suggests that this method of cell volume analysis can be used to improve diagnostic reproducibility in chronic B-cell leukemia.
Orazi
A
A
Department of Anatomic Pathology and Cytology, National Cancer Institute, Milan, Italy.
Cattoretti
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G
Rilke
F
F
D000911:Antibodies, Monoclonal; D001331:Automation; D001772:Blood Cell Count / Q000295:instrumentation; D003937:Diagnosis, Differential; D006801:Humans; D015448:Leukemia, B-Cell / Q000097:blood / Q000175:diagnosis*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000097:blood / Q000175:diagnosis*
D000911:Antibodies, Monoclonal