title: Antioxidant enzymes and lipoperoxide in blood in uremic children and adolescents.
abstract: To determine whether oxidant-antioxidant balance is altered in chronic renal failure, antioxidant enzymes and lipid peroxide in peripheral blood cells and lipid peroxide in plasma were measured. Nine children and adolescents maintained on hemodialysis (HD), 9 on continuous ambulatory peritoneal dialysis (CAPD), and 14 controls were studied. Lipid peroxide was assayed fluorimetrically as thiobarbituric acid-reactive substances, superoxide dismutases by radioimmunoassays. Both manganese and copper-zinc superoxide dismutases in lymphocytes and monocytes in the HD and CAPD patients, and manganese superoxide dismutase in polymorphs in the HD patients were higher than in the controls. Copper-zinc superoxide dismutase, glutathione peroxidase, and catalase in erythrocytes were unaltered. The lipid peroxide level in plasma in the dialyzed patients was increased, whereas those in polymorphs and lymphocytes were unaltered. Triglyceride and total cholesterol in plasma in the dialyzed patients were also increased. The plasma lipid peroxide in the patients correlated with the triglyceride and total cholesterol level. This is the first study in which manganese superoxide dismutase is measured in nucleated cells of the patients with chronic renal failure. The present results suggest that increased superoxide dismutases protect against oxidative stress induced by chronic renal failure in nucleated cells but in neither erythrocytes nor plasma.
authors.affiliation: Department of Pediatrics, Yamanashi Medical College, Tamahocho, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Asayama
authors.forename: Y
authors.initials: Y
authors.lastname: Shiki
authors.forename: H
authors.initials: H
authors.lastname: Ito
authors.forename: O
authors.initials: O
authors.lastname: Hasegawa
authors.forename: A
authors.initials: A
authors.lastname: Miyao
authors.forename: H
authors.initials: H
authors.lastname: Hayashibe
authors.forename: K
authors.initials: K
authors.lastname: Dobashi
authors.forename: K
authors.initials: K
authors.lastname: Kato
mesh_terms: D000293:Adolescent; D000328:Adult; D000975:Antioxidants; D002648:Child; D002675:Child, Preschool; D005260:Female; D005609:Free Radicals; D006801:Humans; D006949:Hyperlipidemias / Q000150:complications / Q000201:enzymology / Q000378:metabolism*; D007676:Kidney Failure, Chronic / Q000150:complications / Q000201:enzymology / Q000378:metabolism*; D008054:Lipid Peroxides / Q000097:blood*; D008297:Male; D010531:Peritoneal Dialysis, Continuous Ambulatory; D013482:Superoxide Dismutase / Q000097:blood*
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D008054:Lipid Peroxides; D013482:Superoxide Dismutase