title: Cleavage of the bacteriophage P1 packaging site (pac) is regulated by adenine methylation.
abstract: The packaging of bacteriophage PI DNA is initiated when the phage packaging site (pac) is recognized and cleaved and continues until the phage head is full. We have previously shown that pac is a 162-base-pair segment of P1 DNA that contains seven DNA adenine methyltransferase methylation sites (5'-GATC). We show here that cleavage of pac is methylation sensitive. Both in vivo and in vitro experiments indicate that methylated pac is cleavable, whereas unmethylated pac is not. Moreover, DNA isolated from P1 phage and containing an uncut pac site was a poor substrate for in vitro cleavage until it was methylated by the Escherichia coli DNA adenine methyltransferase. Comparison of that uncut pac DNA with other viral DNA fragments by digestion with methylation-sensitive restriction enzymes indicated that the uncut pac DNA was preferentially undermethylated. In contrast, virion DNA containing a cut pac site was not undermethylated. We believe these results indicate that pac cleavage is regulated by adenine methylation during the phage lytic cycle.
authors.affiliation: E. I. duPont de Nemours & Co. Inc., Central Research and Development Department, Wilmington, DE 19880-0328.
authors.forename: N
authors.initials: N
authors.lastname: Sternberg
authors.forename: J
authors.initials: J
authors.lastname: Coulby
mesh_terms: D000225:Adenine; D001482:Base Composition; D001483:Base Sequence; D003090:Coliphages / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D015967:Gene Expression Regulation, Viral; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)
chemical_list: D004279:DNA, Viral; D029968:Escherichia coli Proteins; D008780:Methyltransferases; C058693:Dam methyltransferase; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); C499294:dam protein, E coli; D000225:Adenine