[Heparin-sepharose affinity chromatography and reduction conditions as a method of a selective process for separate isoforms of apolipoprotein A].
Gel permeation chromatography of VLDL apoproteins on Sepharose CL-6B in denaturing conditions and affinity chromatography on heparin-sepharose 4B in the presence of reducing agent dithiothreitol were used for preparative isolation of apolipoprotein E of high purity from human plasma VLDL. Sequential elution of apolipoprotein E from affinity column using increasing ionic strength solutions (0.4 M NaCl and 1.0 M NaCl) permitted to obtain "high affinity" apo E preparation with increased relative apoprotein isoform content with the highest positive charge.
Dergunov
A D
AD
Aniskovich
L P
LP
Shuvaev
V V
VV
D001057:Apolipoproteins E / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D004229:Dithiothreitol / Q000494:pharmacology; D006493:Heparin; D006801:Humans; D007525:Isoelectric Focusing; D008079:Lipoproteins, VLDL / Q000032:analysis; D012685:Sepharose
D001057:Apolipoproteins E; D008079:Lipoproteins, VLDL; D006493:Heparin; D012685:Sepharose; D004229:Dithiothreitol
Affinnaia khromatografiia na geparin-sefaroze v redutsiruiushchikh usloviiakh kak sposob izbiratel'nogo obogashcheniia po otdel'nym izoformam apolipoproteina E.