Myocardial accumulation of iodinated beta-methyl-branched fatty acid analogue, iodine-125-15-(p-iodophenyl)-3-(R,S)methylpentadecanoic acid (BMIPP), in relation to ATP concentration.
To clarify the relationship between the myocardial accumulation of 125I-15-(p-iodophenyl)-3-(R,S)-methylpentadecanoic acid (BMIPP) and intracellular adenosine-5'-triphosphate (ATP) content, the effect of 2,4-dinitrophenol (DNP, an electron transport uncoupler) on myocardial BMIPP accumulation was studied, in comparison with that of thallium-201-chloride (201Tl-Cl). In the mouse myocardium, DNP decreased the intracellular ATP and ADP levels, without affecting either acyl-CoA synthetase activity or the level of CoA-SH. Following treatment with DNP, decreases in myocardial BMIPP accumulation correlated well with those of ATP, while 201Tl-Cl showed slightly increased accumulation in the myocardium. Thus, in some diseases, BMIPP may be useful in evaluating myocardial ATP levels.
Fujibayashi
Y
Y
Department of Nuclear Medicine, School of Medicine, Kyoto University, Japan.
Yonekura
Y
Y
Takemura
Y
Y
Wada
K
K
Matsumoto
K
K
Tamaki
N
N
Yamamoto
K
K
Konishi
J
J
Yokoyama
A
A
D019297:2,4-Dinitrophenol; D000244:Adenosine Diphosphate / Q000032:analysis; D000249:Adenosine Monophosphate / Q000032:analysis; D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D003065:Coenzyme A / Q000032:analysis; D003066:Coenzyme A Ligases / Q000378:metabolism; D004140:Dinitrophenols / Q000494:pharmacology; D005227:Fatty Acids; D006321:Heart / Q000000981:diagnostic imaging* / Q000187:drug effects; D007457:Iodine Radioisotopes; D007462:Iodobenzenes / Q000737:chemistry / Q000493:pharmacokinetics*; D008297:Male; D051379:Mice; D009206:Myocardium / Q000737:chemistry / Q000378:metabolism*; D011877:Radionuclide Imaging; D013793:Thallium / Q000493:pharmacokinetics; D013794:Thallium Radioisotopes / Q000493:pharmacokinetics; D014018:Tissue Distribution; D014475:Uncoupling Agents / Q000494:pharmacology
D004140:Dinitrophenols; D005227:Fatty Acids; D007457:Iodine Radioisotopes; D007462:Iodobenzenes; D013794:Thallium Radioisotopes; D014475:Uncoupling Agents; C049338:iodofiltic acid; C027549:thallium chloride; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D013793:Thallium; D003066:Coenzyme A Ligases; D019297:2,4-Dinitrophenol; D003065:Coenzyme A