Fluoride stimulates [3H]thymidine incorporation and alkaline phosphatase production by human osteoblasts.
The effect of sodium fluoride on alkaline phosphatase (ALP) release and [3H]thymidine uptake by human osteoblasts in culture was investigated. Sodium fluoride stimulated both ALP release and [3H]thymidine uptake at concentrations of sodium fluoride greater than 250 mumol/L. This stimulation was similar in magnitude to that induced by 1,25-dihydroxycholecalciferol. The fluoride-induced increase in ALP was inhibited by verapamil, a calcium channel blocker. We conclude that sodium fluoride stimulates osteoblasts to proliferate and to release ALP. This stimulation by fluoride is dependent on calcium influx. Fluoride-induced stimulation of human osteoblasts may be relevant to its effect in enhancing bone formation in patients with osteoporosis.
Khokher
M A
MA
Department of Chemical Pathology and Human Metabolism, Royal Free Hospital, London, UK.
Dandona
P
P
D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D002478:Cells, Cultured; D005459:Fluorides / Q000494:pharmacology*; D006801:Humans; D010006:Osteoblasts / Q000378:metabolism*; D013268:Stimulation, Chemical; D013936:Thymidine / Q000493:pharmacokinetics*; D014316:Tritium
D014316:Tritium; D000469:Alkaline Phosphatase; D005459:Fluorides; D013936:Thymidine