title: [Laser spectroscopic assessment of induced hyperoxia--an animal experiment in lambs].
abstract: The noninvasive determination of biochemical parameters has become an important aspect of intensive care medicine. The newly developed monitors for laser reflectometry provide the possibility of spectroscopic monitoring. The equipment consists of a near-infrared data collection unit and a personal computer. The four laser diodes emit light at wavelengths of 775, 805, 845 and 904 nm. By analyzing the changes in optical density during laser irradiation of biological tissue, information is obtained about the relative changes in the concentration of hemoglobin and the blood volume. In animal experiments with ten fetal lambs we evaluated the reliability of near infrared laser spectroscopy. Fetal hyperoxia was achieved by means of an extracorporeal circuit with interposition of a membrane oxygenator (0.8 m2, Scimed). During the induced hyperoxia the laser spectroscopic tracings showed a rise in the HbO2 signal with a synchronous decrease in the HbR signal. Additionally, the spectroscopic pattern showed a characteristic initial rise in the intracerebral blood volume, which stabilized after 4 minutes. We found a significant correlation between the intermittently measured PO2 values of the arterial blood samples and the laser spectroscopic HbO2 and HbR signals (r = 0.87, and r = -0.82, respectively; p less than 0.001). Furthermore, hyperoxia was indicated by the laser system with a short lag time. We conclude that laser spectroscopy is a reliable method with a high potential for clinical routine use in intensive care, as it provides noninvasive continuous information at comparatively low costs using portable monitors.
authors.affiliation: Universitäts-Frauenklinik Bonn.
authors.forename: S
authors.initials: S
authors.lastname: Schmidt
authors.forename: W
authors.initials: W
authors.lastname: Decleer
authors.forename: S
authors.initials: S
authors.lastname: Gorissen-Bosselmann
authors.forename: H
authors.initials: H
authors.lastname: Eilers
authors.forename: K
authors.initials: K
authors.lastname: Pringle
authors.forename: N
authors.initials: N
authors.lastname: Helledie
authors.forename: P
authors.initials: P
authors.lastname: Rolfe
authors.forename: D
authors.initials: D
authors.lastname: Krebs
mesh_terms: D000818:Animals; D001810:Blood Volume; D001921:Brain / Q000098:blood supply; D015199:Extracorporeal Membrane Oxygenation; D005333:Fetus; D007834:Lasers; D010100:Oxygen / Q000097:blood*; D010108:Oxyhemoglobins / Q000032:analysis; D012756:Sheep / Q000097:blood*; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D010108:Oxyhemoglobins; D010100:Oxygen
vernacular_title: Laserspektroskopische Erfassung der induzierten Hyperoxie--eine tierexperimentelle Studie beim Lamm.