Blood-glucose-lowering activity of 2-(3-phenylpropoxyimido)-butyrate (BM 13.677).
A single oral or intraperitoneal application of 2-(3-phenylpropoxyimido)-butyrate (BM 13.677) resulted in a dose-dependent blood-glucose-lowering effect in fasted guinea-pigs. The threshold dose and the EC50 were estimated as 25 mg/kg and 63 mg/kg, respectively, which is between that of the biguanides phenformin and metformin. A rise in blood lactate concentrations was observed only at high doses of BM 13.677, but was not related to an irreversible metabolic inhibition. Among several rodent species studied the potency of the drug decreased in the order guinea-pig much greater than mouse greater than rat = rabbit. Inhibition of hepatic gluconeogenesis by the drug was demonstrated in the perfused liver or hepatocytes of guinea-pigs. Inhibition of glucose production by the perfused liver in the presence of 0.1 mM BM 13.677 was dependent on the substrate and decreased in the order: lactate greater than pyruvate greater than alanine much greater than propionate greater than glycerol = fructose. This suggests a specific interaction of the drug with a mitochondrial key reaction of gluconeogenesis. Stimulation of glucose oxidation in rat diaphragm by the compound (EC50 = 0.85 mM) suggests that besides inhibition of gluconeogenesis also extrahepatic effects contribute to the blood-glucose-lowering effects of the drug.
Kühnle
H F
HF
Boehringer Mannheim GmbH, Dept. Medical Research, Mannheim, Federal Republic of Germany.
Wolff
H P
HP
Schmidt
F H
FH
Reiter
R
R
D000818:Animals; D002478:Cells, Cultured; D005215:Fasting; D005943:Gluconeogenesis / Q000187:drug effects*; D006168:Guinea Pigs; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007098:Imino Acids / Q000008:administration & dosage / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects*; D008297:Male; D008687:Metformin / Q000494:pharmacology; D051379:Mice; D010629:Phenformin / Q000494:pharmacology; D011817:Rabbits; D051381:Rats
D007004:Hypoglycemic Agents; D007098:Imino Acids; C065280:BM 13677; D008687:Metformin; D010629:Phenformin