[Comparison by 31P magnetic resonance spectroscopy of effect of simple immersion and continuous perfusion methods on organ viability of preserved rat hearts].
31P magnetic resonance spectroscopy was used to measure high energy phosphates of the heart continuously as an index of the viability of the preserved organ together with the cardiac function after preservation. Results with the simple immersion (SI) and the continuous perfusion (CP) methods were compared. With SI, ATP decreased to 14.8 +/- 4.1% of the base line after 12 hr of preservation, and phosphocreatine decreased to 27.8 +/- 6.2% after 3 hr, remaining at about 30% of the base line thereafter. With CP, ATP and phosphocreatine were unchanged even after 24 hr of preservation; they were 99.1 +/- 2.6% and 119.6 +/- 6.7%, respectively. The ATP/Pi ratio was 5.4 +/- 0.7% after 12 hr with SI and 62.2 +/- 5.8% after 24 hr with CP. LV dP/dt was 96.6 +/- 11.5% after 3 hr of SI (3-SI), which was not significantly different from the control. After 6 hr of SI (6-SI), the value was 122.7 +/- 0.2%, significantly higher (p less than 0.01) than the control. After 9 hr of SI (9-SI) or after 24 hr of CP (24-CP), it was 80.8 +/- 4.6% and 67.8 +/- 8.2%, respectively, both were significantly lower (p less than 0.01) than the control. The rate-pressure product returned to the control level after 3-SI and 6-SI, but after 9-SI or 24-CP, it was 74.2 +/- 3.7% and 53.0 +/- 11.0%, and they were significantly (p less than 0.01) lower than the control.(ABSTRACT TRUNCATED AT 250 WORDS)
Iwamoto
K
K
Department of Surgery, Osaka City University Medical School, Japan.
Usui
N
N
Kinoshita
H
H
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D003080:Cold Temperature; D006321:Heart / Q000502:physiology*; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009206:Myocardium / Q000378:metabolism; D009926:Organ Preservation / Q000379:methods*; D010477:Perfusion; D010761:Phosphorus Radioisotopes; D051381:Rats; D011919:Rats, Inbred Strains; D014022:Tissue Survival
D010761:Phosphorus Radioisotopes; D000255:Adenosine Triphosphate