Synthetic sialyl glycolipids (sialo-cholesterol and sialo-diglyceride) induce granulocytic differentiation of human myelogenous leukemia cell line HL-60.
When HL-60 cells were cultivated with synthetic sialyl glycolipids, sialo-cholesterol and sialo-diglyceride, the cells were found to be differentiated into mature granulocytes on morphological and functional criteria. The differentiation of cells was accompanied by inhibition of cell proliferation. The differentiation-inducing activity of sialo-cholesterol was greater than that of sialo-diglyceride on a molar basis, and the alpha-anomer of each compound was more potent than the beta-anomer, suggesting that the stereospecific structure of the compounds is important for the differentiation-inducing activity.
Saito
M
M
Division of Hemopoiesis, Jichi Medical School, Tochigi, Japan.
Nojiri
H
H
Ogino
H
H
Yuo
A
A
Ogura
H
H
Itoh
M
M
Tomita
K
K
Ogawa
T
T
Nagai
Y
Y
Kitagawa
S
S
D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D004950:Esterases / Q000378:metabolism; D006017:Glycolipids / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D007938:Leukemia / Q000378:metabolism* / Q000473:pathology; D008657:Metabolic Clearance Rate; D010100:Oxygen / Q000378:metabolism; D014407:Tumor Cells, Cultured
D006017:Glycolipids; C009255:sialoglycolipids; D004950:Esterases; D010100:Oxygen