title: Induction of DNA-repair synthesis in primary rat hepatocytes by epoxides.
abstract: The genotoxicity of 10 epoxides was investigated in the UDS test with primary rat hepatocytes. The sensitivity of the assay was demonstrated using 2-acetylaminofluorence. The epoxides 1,2-epoxyoctane, 1,2-epoxydecane, epoxycyclooctane, epoxycyclododecane, (+)-limoneoxide, alpha-pinaneoxide, transstilbeneoxide, and cis-2,3-epoxysuccinic acid, which are known to be non-mutagenic in the Ames test, as well as the bacterial mutagen, 1,2-epoxyphenoxypropane did not induce UDS in primary hepatocytes of the rat. However, a positive UDS response obtained with glycidyltrimethylammonium chloride showed that metabolic inactivation of the oxirane ring in hepatocytes is influenced by further structural substituents.
authors.affiliation: Institut für Allgemeine Genetik, Freie Universität Berlin, Germany.
authors.forename: W
authors.initials: W
authors.lastname: von der Hude
authors.forename: R
authors.initials: R
authors.lastname: Mateblowski
authors.forename: A
authors.initials: A
authors.lastname: Basler
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004260:DNA Repair / Q000187:drug effects*; D004852:Epoxy Compounds / Q000494:pharmacology*; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004852:Epoxy Compounds