Adult expression of the Drosophila Lsp-2 gene.
Previous work has demonstrated expression of the larval serum protein-2 gene, Lsp-2, uniquely during the late larval and pupal stages of development in Drosophila melanogaster. Here we report that the LSP-2 polypeptide accumulates in the hemolymph throughout adult life as well. Western blot analysis using an LSP-2 antiserum reveals notable differences in the molecular weight of the larval and adult polypeptides. Lsp-2 transcription results in a unique mRNA of 2.3 kb, exhibiting the same 5' end in both larvae and adults. However, adult Lsp-2 mRNa is only expressed at 1% of the high level detectable in late third-instar larvae. Whereas Lsp-2 mRNA accumulates uniformly in all fat body cells of third-instar larvae, over 80% of the adult Lsp-2 transcript is expressed in the adipose tissue of the head. These results suggest a differential regulation for expression of the Drosophila Lsp-2 gene in adults and larvae.
Benes
H
H
Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock 72205.
Edmondson
R G
RG
Fink
P
P
Kejzlarová-Lepesant
J
J
Lepesant
J A
JA
Miles
J P
JP
Spivey
D W
DW
D000818:Animals; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins; D005216:Fat Body / Q000378:metabolism; D015870:Gene Expression; D006458:Hemolymph / Q000378:metabolism; D007301:Insect Hormones / Q000096:biosynthesis / Q000235:genetics*; D007814:Larva / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*
D029721:Drosophila Proteins; D007301:Insect Hormones; D012333:RNA, Messenger; C034648:larval serum protein, Drosophila