Perinatal infection of human T-lymphotropic virus type I, the etiologic virus of adult T-cell leukemia/lymphoma. DNA amplification of specific human T-lymphotropic virus type I sequences.
A gene amplification technique, polymerase chain reaction, was used to detect human T-lymphotropic virus type I (HTLV-I), the etiologic agent of adult T-cell leukemia/lymphoma, in mononuclear cells in peripheral blood and breast milk of ten HTLV-I carrier gravida. The DNA in umbilical cord blood mononuclear cells of the neonates born to the HTLV-I carrier gravida were also amplified and examined for the possibility of HTLV-I infection via placenta during pregnancy. The HTLV-I sequences were detected both in the peripheral blood and milk of all ten carrier gravida by Southern blot analysis of amplified DNA. However, HTLV-I proviral DNA could not be detected in the cord blood of the carriers' neonates, indicating that transplacental infection of HTLV-I should be rare and that postpartum infection via breast milk is a likely major perinatal transmission route.
Saji
F
F
Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
Ohashi
K
K
Tokugawa
Y
Y
Kamiura
S
S
Azuma
C
C
Tanizawa
O
O
D000912:Antibodies, Neoplasm / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology; D001483:Base Sequence; D015139:Blotting, Southern; D001942:Breast Feeding; D005260:Female; D005312:Fetal Blood / Q000382:microbiology; D005784:Gene Amplification; D015490:HTLV-I Infections / Q000097:blood / Q000635:transmission; D015368:Human T-lymphotropic virus 1 / Q000502:physiology*; D006801:Humans; D007231:Infant, Newborn; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000209:etiology / Q000382:microbiology*; D007963:Leukocytes, Mononuclear / Q000502:physiology; D008895:Milk, Human / Q000382:microbiology; D008969:Molecular Sequence Data; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000209:etiology / Q000382:microbiology*
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm