title: Carbonyl reductases from rat testis and vas deferens. Purification, properties and localization.
abstract: Three enzyme forms (T1, T2, T3) from rat testis and two from rat vas deferens (V1, V2) of carbonyl reductase have been highly purified to apparent homogeneity. These carbonyl reductases from rat reproductive organs have several similarities in terms of molecular mass (32-33 kDa), isoelectric point (pI 5.9-6.4), immunochemical properties, cofactor requirement (NADPH dependency) and sensitivity to sulfhydryl reagents. The isoenzymes from the vas deferens (V1, V2) have similar catalytic activities, whereas those from the testis (T1, T2, T3) showed different catalytic activities from each other. All enzymes, however, reduced quinones, aromatic aldehydes and ketones, while T3, V1 and V2 were characterized as possessing high affinity towards prostaglandins. An immunoinhibition study using a specific antibody indicated that these enzymes were solely responsible for the overall catalytic activities of 13, 14-dihydro-15-oxo-prostaglandin F2 alpha, 4-benzoylpyridine, and 4-nitroacetophenone reduction and prostaglandin F2 alpha oxidation in both testis and vas deferens cytosol. The immunohistochemical staining revealed a positive immunoreactivity to antibody only in the Leydig cells of the testis, but neither the germ cells nor Sertoli cells in the seminiferous tubule. The staining also showed that the enzymes in the vas deferens were primarily localized in mucosal epithelium cells.
authors.affiliation: Department of Pharmacology, Tokyo College of Pharmacy, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Iwata
authors.forename: N
authors.initials: N
authors.lastname: Inazu
authors.forename: S
authors.initials: S
authors.lastname: Takeo
authors.forename: T
authors.initials: T
authors.lastname: Satoh
mesh_terms: D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D007124:Immunoenzyme Techniques; D007700:Kinetics; D008297:Male; D008970:Molecular Weight; D051381:Rats; D013379:Substrate Specificity; D013737:Testis / Q000201:enzymology*; D014649:Vas Deferens / Q000201:enzymology*
chemical_list: D000429:Alcohol Oxidoreductases