Structure of the type 5 capsular polysaccharide of Staphylococcus aureus.
The Staphylococcus aureus type 5 capsular polysaccharide is composed of 2-acetamido-2-deoxy-L-fucose (1 part), 2-acetamido-2-deoxy-D-fucose (1 part), and 2-acetamido-2-deoxy-D-mannuronic acid (1 part). On the basis of methylation analysis, optical rotation, high-field one- and two-dimensional 1H- and 13C-n.m.r. experiments, and selective cleavage with 70% aqueous hydrogen fluoride, the polysaccharide was found to be a partially O-acetylated (50%) polymer of the repeating trisaccharide unit, [----4)-3-O-Ac-beta-D-ManpNAcA-(1----4)-a-L-FucpNAc-(1----3) -beta-D-FucpNAc-(1----]n.
Moreau
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Office of Biologics Research and Review, Food and Drug Administration, Bethesda, Maryland 20892.
Richards
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JC
Fournier
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JM
Byrd
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Karakawa
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Vann
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D002240:Carbohydrate Sequence; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D003641:Deamination; D004590:Electrophoresis, Paper; D006858:Hydrofluoric Acid; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009608:Nitrous Acid; D011135:Polysaccharides, Bacterial; D013211:Staphylococcus aureus
D011135:Polysaccharides, Bacterial; D006858:Hydrofluoric Acid; D009608:Nitrous Acid