title: Large scale purification and immunolocalization of bovine uroplakins I, II, and III. Molecular markers of urothelial differentiation.
abstract: The differentiation of mammalian urothelium culminates in the formation of asymmetrical unit membrane (AUM). Using gradient centrifugation and detergent wash, we purified milligram quantities of AUMs which, interestingly, contained three major proteins (15, 27, and 47 kDa) that appeared to be identical to the three immunoaffinity purified, putatively AUM-associated proteins that we described earlier (Yu, J., Manabe, M., Wu, X.-R., Xu, C., Surya, B., and Sun, T.-T. (1990) J. Cell Biol., 111, 1207-1216). Peptide mapping and immunoblotting established that these three proteins were distinct molecules. Using monospecific antibodies to these three proteins, we showed that they were all restricted to the superficial urothelial cells and were AUM-associated. The 27- and 15-kDa proteins were detected exclusively on the luminal side of mature, apical AUMs. In contrast, epitopes of the 47-kDa protein were detected on both sides of apical AUMs suggesting a transmembranous configuration. These results (i) provide the strongest evidence thus far that AUM contains three major proteins (the 27-kDa uroplakin I, 15-kDa uroplakin II, and 47-kDa uroplakin III) which form an extremely insoluble complex, (ii) suggest that uroplakin II, like uroplakin I (Yu, J., Manabe, M., Wu, X.-R., Xu, C., Surya, B., and Sun, T.-T. (1990) J. Cell. Biol. 111, 1207-1216), translocates from one side of the membrane to another during AUM maturation, (iii) indicate that uroplakin III may play a different structural role than uroplakins I and II in AUM formation, and (iv) establish the three uroplakins as markers for an advanced stage of urothelial differentiation.
authors.affiliation: Department of Dermatology, Kaplan Cancer Center, New York University School of Medicine, New York 10016.
authors.forename: X R
authors.initials: XR
authors.lastname: Wu
authors.forename: M
authors.initials: M
authors.lastname: Manabe
authors.forename: J
authors.initials: J
authors.lastname: Yu
authors.forename: T T
authors.initials: TT
authors.lastname: Sun
mesh_terms: D000818:Animals; D000906:Antibodies; D015415:Biomarkers / Q000737:chemistry*; D002417:Cattle; D002454:Cell Differentiation; D002462:Cell Membrane / Q000648:ultrastructure; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells; D004848:Epithelium / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D008565:Membrane Proteins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D008854:Microscopy, Electron; D008970:Molecular Weight; D010446:Peptide Fragments / Q000302:isolation & purification; D001743:Urinary Bladder / Q000166:cytology* / Q000648:ultrastructure
chemical_list: D000906:Antibodies; D015415:Biomarkers; D008565:Membrane Proteins; D010446:Peptide Fragments