title: Mouse mammary carcinoma delta-aminolevulinate dehydratase.
abstract: 1. Aminolevulinate dehydratase (ALA-D) was studied in crude extract from mouse mammary carcinoma, normal mouse liver and tumour bearing mouse liver. 2. A Michaelis-Menten behaviour and Km values between 0.24 and 0.31 mM were obtained for the enzyme in either source. 3. In all three tissues there was a linear relationship between porphobilinogen formation and incubation time, up to 120 min, ALA-D was thermostable and optimum pH was at 6.8. 4. There seems to be no structural alterations in tumoural ALA-D as compared with the enzyme from liver of both normal and tumour bearing mice.
authors.affiliation: Centro de Investigaciones sobre Porfirinas y Porfirias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
authors.forename: N M
authors.initials: NM
authors.lastname: Navone
authors.forename: C F
authors.initials: CF
authors.lastname: Polo
authors.forename: A L
authors.initials: AL
authors.lastname: Frisardi
authors.forename: A M
authors.initials: AM
authors.lastname: Batlle
mesh_terms: D000818:Animals; D004795:Enzyme Stability; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000201:enzymology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011162:Porphobilinogen / Q000378:metabolism; D000623:Porphobilinogen Synthase / Q000378:metabolism*
chemical_list: D011162:Porphobilinogen; D000623:Porphobilinogen Synthase