title: Detection of colonization factor antigen I-positive enterotoxigenic Escherichia coli with a cloned polynucleotide probe.
abstract: We compared a new colony hybridization assay with an established enzyme-linked immunosorbent assay for detection of enterotoxigenic Escherichia coli (ETEC) expressing colonization factor antigen I (CFA/I). The tests were applied to 135 human ETEC strains. Of these isolates, 30 had previously been characterized for CFAs. A strain harboring the plasmid vector of the polynucleotide gene probe, nine non-ETEC strains from healthy infants, and eight ETEC strains of animal origin were included for further evaluation of probe specificity. The two assays showed a high level of concordance in the specific detection of ETEC strains expressing CFA/I. A total of 24 strains tested positive in the CFA/I hybridization assay, while 23 of those strains were positive in the CFA/I enzyme-linked immunosorbent assay. The single discrepant result could be explained by the loss of a regulatory gene. The strain harboring the plasmid vector of the probe, the non-ETEC E. coli strains, and the ETEC strains of animal origin were all negative in the CFA/I probe assay.
authors.affiliation: Institute of International Health and Medical Department B, Haukeland Hospital, Bergen, Norway.
authors.forename: H M
authors.initials: HM
authors.lastname: Grewal
authors.forename: H
authors.initials: H
authors.lastname: Sommerfelt
authors.forename: W
authors.initials: W
authors.lastname: Gaastra
authors.forename: A M
authors.initials: AM
authors.lastname: Svennerholm
authors.forename: M K
authors.initials: MK
authors.lastname: Bhan
authors.forename: A M
authors.initials: AM
authors.lastname: Hamers
authors.forename: R
authors.initials: R
authors.lastname: Kumar
authors.forename: G
authors.initials: G
authors.lastname: Wiklund
authors.forename: B
authors.initials: B
authors.lastname: Bjorvatn
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics* / Q000378:metabolism; D015342:DNA Probes; D004768:Enterotoxins / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D005069:Evaluation Studies as Topic; D036781:Fimbriae Proteins; D006801:Humans; D009693:Nucleic Acid Hybridization
chemical_list: D000942:Antigens, Bacterial; D015342:DNA Probes; D004768:Enterotoxins; C022924:colonization factor antigens; D036781:Fimbriae Proteins