Inhibitory effects of ulinastatin on the production of cytokines: implications for the prevention of septicemic shock.
The study was designed to determine whether ulinastatin can be used as a biological response modifier for the prevention of septicemic shock. Monocytes from heparinized blood of three healthy volunteers were incubated with 0.125 or 1.25 U/ml of ulinastatin, and then endotoxin (Escherichia coli lipopolysaccharide) was added at concentrations of 0.1, 1.0, and 10.0 micrograms/ml. A dose-dependent increase in the production of tumor necrosis factor alpha, interleukin-1 alpha, and interleukin-1 beta was noted after endotoxin stimulation. Production of these cytokines was inhibited by the addition of ulinastatin in a dose-dependent manner. The results indicate that ulinastatin could be useful in the prevention of septicemic shock.
Endo
S
S
Critical Care and Emergency Center, Iwate Medical University, Morioka, Japan.
Inada
K
K
Taki
K
K
Hoshi
S
S
Yoshida
M
M
D016207:Cytokines / Q000096:biosynthesis*; D006023:Glycoproteins / Q000494:pharmacology*; D006801:Humans; D007155:Immunologic Factors; D012720:Severity of Illness Index; D012772:Shock, Septic / Q000517:prevention & control*
D016207:Cytokines; D006023:Glycoproteins; D007155:Immunologic Factors; C028665:urinastatin