Vitamin A status in preschool-age Indonesian children as assessed by the modified relative-dose-response assay.
The modified relative-dose-response (MRDR) assay has been validated in rats as a function of vitamin A status and tested in a group of American children. In this study the MRDR assay was applied to West Javan children who are at risk of being vitamin A deficient. Of 86 children enrolled, 75 were tested. In a time-course study involving 22 children aged 3.7-5.3 y, blood samples were taken at different times after doses of 0.35 mumol 3,4-didehydroretinyl acetate/kg body wt. Generally, the ratio of dehydroretinol to retinol (DR-R ratio) peaked between 4 and 8 h. Thereafter, in a survey of 53 children aged 0.6-4.8 y, single blood samples were drawn 5 h after the dehydroretinyl acetate dose. The DR-R ratio ranged from 0.0028 to 0.169. With a DR-R ratio of 0.03 as the cutoff value, 62% of the children were judged to be of marginal vitamin A status.
Tanumihardjo
S A
SA
Department of Biochemistry and Biophysics, Iowa State University, Ames 50011.
Muhilal
Yuniar
Y
Y
Permaesih
D
D
Sulaiman
Z
Z
Karyadi
D
D
Olson
J A
JA
D000886:Anthropometry; D002675:Child, Preschool; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007214:Indonesia; D007223:Infant; D009752:Nutritional Status; D013997:Time Factors; D014212:Tretinoin / Q000031:analogs & derivatives*; D014801:Vitamin A / Q000031:analogs & derivatives / Q000097:blood*; D014802:Vitamin A Deficiency / Q000175:diagnosis*
C024366:vitamin A2; D014801:Vitamin A; C064742:3,4-didehydroretinoic acid; D014212:Tretinoin