Hepatitis B virus propagated in a rat hepatoma cell line is infectious in a primate model.
The human hepatitis B virus (HBV) produced by a rat hepatoma cell line through transfection with HBV DNA is infectious in the human primate model--chimpanzee. Since hepadnaviruses are known to have an extremely narrow host range, our results support the idea that the host species barrier of HBV infection resides on the penetration/adsorption step rather than any postpenetration intracellular event during the virus life cycle.
Shih
C
C
Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia 19104-6059.
Yu
M Y
MY
Li
L S
LS
Shih
J W
JW
D000818:Animals; D015139:Blotting, Southern; D004279:DNA, Viral / Q000235:genetics; D006509:Hepatitis B / Q000276:immunology / Q000382:microbiology* / Q000635:transmission; D006510:Hepatitis B Antibodies / Q000378:metabolism; D006513:Hepatitis B e Antigens / Q000378:metabolism; D006515:Hepatitis B virus / Q000254:growth & development* / Q000472:pathogenicity; D008114:Liver Neoplasms, Experimental / Q000382:microbiology*; D002679:Pan troglodytes / Q000382:microbiology; D051381:Rats; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D004279:DNA, Viral; D006510:Hepatitis B Antibodies; D006513:Hepatitis B e Antigens