Hormone-free mouse glucocorticoid receptors overexpressed in Chinese hamster ovary cells are localized to the nucleus and are associated with both hsp70 and hsp90.
In this work, we examine the cellular localization and protein interactions of mouse glucocorticoid receptors that have been overexpressed in Chinese hamster ovary (CHO) cells (Hirst, M. A., Northrop, J. P., Danielsen, M., and Ringold, G. M. (1990) Mol. Endocrinol. 4, 162-170). We demonstrate that wild-type unliganded mouse glucocorticoid receptor, which is expressed in CHO cells to a level approximately 10 times that of L cells, is localized entirely to the nucleus by indirect immunofluorescence with the BuGR antireceptor monoclonal antibody. Overexpressed receptors that have either no hormone binding activity or no DNA binding activity because of point mutations also localize to the nucleus, providing genetic proof that the nuclear localization cannot reflect a steroid-mediated shift of the receptor from the cytoplasm to the nucleus and that DNA binding activity is not required for nuclear localization. Like unliganded progesterone receptors, which also associate in a loosely bound "docking" complex with the nucleus, the mouse glucocorticoid receptor overexpressed in CHO cells is associated with both hsp90 and hsp70. This is in contrast to the untransformed mouse glucocorticoid receptor in L cell cytosol, which is associated with hsp90 but not hsp70. The difference in hsp70 association between cell types could reflect overexpression of the receptor in CHO cells. However, like receptors in CHO cells selected for very high levels of overexpression, receptors in CHO cells selected for an intermediate level of receptor expression that is comparable to that of L cells are also bound to hsp70. This observation argues against an explanation of hsp70 association based purely on receptor overexpression, and we speculate that association of the unliganded glucocorticoid receptor with hsp70 might be a consequence of its nuclear localization in the CHO cells. Although there are differences between the mouse receptor in CHO cells and L cells, the nuclear localization signal of the untransformed mouse receptor reacts equivalently with the AP64 antibody against NL1 in cytosols prepared from both cell types.
Sanchez
E R
ER
Department of Pharmacology, University of Michigan Medical School, Ann Arbor 48109.
Hirst
M
M
Scherrer
L C
LC
Tang
H Y
HY
Welsh
M J
MJ
Harmon
J M
JM
Simons
S S
SS
Ringold
G M
GM
Pratt
W B
WB
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D005455:Fluorescent Antibody Technique; D005784:Gene Amplification; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000032:analysis / Q000378:metabolism*; D007739:L Cells / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D010053:Ovary; D011965:Receptors, Glucocorticoid / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014222:Triamcinolone Acetonide / Q000378:metabolism
D006360:Heat-Shock Proteins; D011965:Receptors, Glucocorticoid; D014222:Triamcinolone Acetonide