Radioimmunological determination of fenoterol. Part II: Antiserum and tracer for the determination of fenoterol.
A radioimmunological method for the determination of the concentration of fenoterol in biological samples is described. The mixture of antibodies against the enantiomers of fenoterol obtained with the selected hapten shows a high affinity for racemic fenoterol and for the monoiodo125-fenoterol used as a tracer, which is also present as a racemate. The limit of detection of the radioimmunoassay for fenoterol (racemate) in biological samples (plasma, urine) is 10-20 pg/ml. The precision and accuracy of the radioimmunoassay, in the presence of racemic fenoterol, are sufficient for an analysis and meaningful interpretation of samples from human pharmacokinetic studies. A relationship between the cross-reactivity of the antibodies against fenoterol and a preferred conformation of the fenoterol molecule in aqueous solution is discussed.
Rominger
K L
KL
Department of Biochemistry, Boehringer Ingelheim KG, Ingelheim/Rhein, Fed. Rep. of Germany.
Mentrup
A
A
Stiasni
M
M
D000818:Animals; D000937:Antigen-Antibody Reactions; D003429:Cross Reactions; D005280:Fenoterol / Q000032:analysis*; D006801:Humans; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D013237:Stereoisomerism
D007457:Iodine Radioisotopes; D005280:Fenoterol