Effect of interleukin-1 on gamete interaction and mouse embryo development.
Early stages of endometriosis have been shown to be associated with infertility. The pathophysiology of this relationship is unclear. To determine if interleukin-1 (IL-1), a peritoneal macrophage product, has any effect on gamete interaction and early embryo development, human recombinant IL-1 was added to the coincubation of gametes in the sperm penetration assay (SPA), human zona pellucida assay (ZPPA) and culture medium (Ham's F-10) used for processing semen samples with the layering method, with analysis of velocity and motility after 24 hours. IL-1 was also added to mouse embryos (two cells) cultured for 72 hours. The results showed that IL-1 caused impairment of SPA and ZPPA when compared to control medium, without significant alterations in sperm velocity and motility. Also, IL-1 demonstrated significant inhibition of mouse embryo development. These results help explain subfertility associated with early stages of endometriosis.
Sueldo
C E
CE
Department of Obstetrics and Gynecology, University of California, San Francisco-Fresno 93702.
Kelly
E
E
Montoro
L
L
Subias
E
E
Baccaro
M
M
Swanson
J A
JA
Steinleitner
A
A
Lambert
H
H
D000818:Animals; D001202:Ascitic Fluid / Q000276:immunology; D005314:Embryonic and Fetal Development; D004715:Endometriosis / Q000503:physiopathology*; D005260:Female; D007247:Infertility, Female / Q000209:etiology; D007375:Interleukin-1 / Q000502:physiology*; D008297:Male; D051379:Mice; D010537:Peritoneum; D011247:Pregnancy; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions
D007375:Interleukin-1