[The use of antibodies against DNA modified by trans-diamminedichloroplatinum for identification of specific DNA sequences].
DNA modified by trans-diamminedichloroplatinum (trans-DDP) is suggested as an effective probe for non-isotopic hybridisation. Highly specific anti trans-DDP-DNA antibodies and peroxidase-conjugated antibodies to antirabbit Ig and protein A system have been used for the hybrids visualisation. This method allows one to detect 2 pg/mm2 DNA.
Kiseleva
V I
VI
Turchinskiĭ
M F
MF
Poverennyĭ
A M
AM
D000906:Antibodies / Q000276:immunology*; D001483:Base Sequence; D002945:Cisplatin / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects / Q000235:genetics* / Q000276:immunology; D015342:DNA Probes; D009693:Nucleic Acid Hybridization; D010957:Plasmids
D000906:Antibodies; D015342:DNA Probes; D004247:DNA; D002945:Cisplatin
Ispol'zovanie antitel k DNK, modifitsirovannoĭ trans-diamminodikhlorpltinoĭ, dlia identifikatsii spetsificheskikh posledovatel'nosteĭ DNK.