Development of B cell lineages during a primary anti-hapten immune response.
Cell lineage relationships were examined in large numbers of hybridomas derived from a single mouse at 7 days and from a single mouse at 12 days after a primary immunization. The properties of these developing lineages provide unique insight into their biologic selection and differentiation. The unique VDJ junctions, the characteristics of the somatic mutations, and the nonproductive H chain gene rearrangements observed at day 12 all indicate that the hybridomas were derived from a limited number of progenitor B cells. Clonally related hybridomas were also observed at day 7. The activation of these lineages occurred very early, because somatic variants were strongly selected for within a few days after the primary immunization.
Tao
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Section of Immunobiology, Yale University, School of Medicine, New Haven, CT 06510.
Bothwell
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AL
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D015139:Blotting, Southern; D002454:Cell Differentiation; D005260:Female; D005803:Genes, Immunoglobulin; D006241:Haptens / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007132:Immunoglobulin Isotypes / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data
D006241:Haptens; D007132:Immunoglobulin Isotypes