Analysis of long terminal repeat circle junctions of human immunodeficiency virus type 1.
Circle junctions of unintegrated human immunodeficiency virus type 1 strain IIIB were analyzed after polymerase chain reaction amplification. Among the 28 colonies sequenced, eight unique circle junction species were detected. Five of the eight species resulted in circle junctions with larger inserts than predicted. A majority of these could result from heterogeneity in generating the U5' long terminal repeat terminus.
Smith
J S
JS
Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854.
Kim
S Y
SY
Roth
M J
MJ
D001483:Base Sequence; D002460:Cell Line; D004261:DNA Replication; D004270:DNA, Circular / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid
D004270:DNA, Circular; D004279:DNA, Viral; D015345:Oligonucleotide Probes