title: 12q13 fragility in a family with recurrent spontaneous abortions: expression of the fragile site under different culture conditions.
abstract: A fragile site at the 12q13 band was found in metaphases from lymphocyte cultures of three members of a family. A comparison of the frequency and expression of the fragile site was carried out on cells cultured in RPM-I 1640 with and without BrdU and in 199 media. The fragile site was not typically folate-sensitive, being expressed in standard medium as well as in cultures after exposure to BrdU.
authors.affiliation: Laboratorio di Citogenetica, Centro Auxologico Italiano, Milano.
authors.forename: D
authors.initials: D
authors.lastname: Giardino
authors.forename: D
authors.initials: D
authors.lastname: Bettio
authors.forename: G
authors.initials: G
authors.lastname: Simoni
mesh_terms: D000026:Abortion, Habitual / Q000209:etiology / Q000235:genetics*; D000328:Adult; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000235:genetics*; D002872:Chromosome Deletion; D025063:Chromosome Disorders; D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility; D002881:Chromosomes, Human, Pair 12 / Q000187:drug effects / Q000648:ultrastructure*; D003470:Culture Media / Q000494:pharmacology; D046508:Culture Techniques / Q000379:methods; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D008297:Male; D010375:Pedigree; D011247:Pregnancy
chemical_list: D003470:Culture Media; D001973:Bromodeoxyuridine