[The cytotoxic effect of anti-Ro- (SS-A) antibodies and UVA light on human endothelial cells in vitro].
There is in photosensitive lupus erythematosus a strong association of high titers of anti-Ro(SS-A) antibodies in the serum of the patient with the development of special skin reactions depending on UV-light. The aim of our experiments was to detect a cytotoxic effect of anti-Ro(SS-A) antibodies and UVA-light on human endothelial cells in vitro (standard trypan blue exclusion test, SEM). After UVA-doses of 1 to 100 J/cm2, which were tested, and influence of serum containing anti-Ro(SS-A) antibodies (62 E, ELISA) membrane destructions of endothelial cells depending on the UVA-dose can be seen, irrespective of the fact whether the antibodies are present throughout the irradiation or added afterwards. This cytotoxic reaction depends on complement. A UVA-dose of 100 J/cm2 causes lethal damage of 40% of the cells. These results were confirmed by scanning electron microscopy.
Lanto
B
B
Klinik und Poliklinik für Hautkrankheiten Bereichs Medizin (Charité) der Humboldt-Universität zu Berlin.
Böhm
F
F
Meffert
H
H
Sönnichsen
N
N
D000974:Antibodies, Antinuclear / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D002470:Cell Survival / Q000528:radiation effects*; D003470:Culture Media; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D008854:Microscopy, Electron; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D014466:Ultraviolet Rays; D000099207:SS-A Antigen
D000974:Antibodies, Antinuclear; D001324:Autoantigens; D003470:Culture Media; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C116006:RO60 protein, human; C035356:SS-A antibodies
Zytotoxische Wirkung von Anti-Ro- (SS-A-) Antikörpern und UVA-Licht auf menschliche Endothelzellen in vitro.