Expression of mammalian renal transporters in Xenopus laevis oocytes.
We have injected mRNA from rabbit renal cortex into Xenopus oocytes and demonstrated the expression of renal carriers for Na(+)-independent transport of L-phenylalanine and L-lysine and Na(+)-dependent transport of L-alanine and succinate. Maximal expression of renal amino acid transporters occurred 6-8 days following mRNA injection. The proteins responsible for transport of these four substrates were translated from mRNAs which are between 1.5 and 3.0 kb. This information serves as a starting point for expression cloning of these transport proteins.
Coady
M J
MJ
Department of Physiology, UCLA School of Medicine 90024-1751.
Pajor
A M
AM
Toloza
E M
EM
Wright
E M
EM
D026905:Amino Acid Transport Systems; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D007672:Kidney Cortex / Q000502:physiology*; D007700:Kinetics; D008845:Microinjections; D008970:Molecular Weight; D009865:Oocytes / Q000502:physiology*; D012333:RNA, Messenger / Q000008:administration & dosage / Q000235:genetics / Q000302:isolation & purification; D011817:Rabbits; D012964:Sodium / Q000494:pharmacology; D014982:Xenopus laevis
D026905:Amino Acid Transport Systems; D000596:Amino Acids; D002352:Carrier Proteins; D012333:RNA, Messenger; D012964:Sodium