The v-rel oncogene encodes a kappa B enhancer binding protein that inhibits NF-kappa B function.
Studies of NF-kappa B suggest that this enhancer binding activity corresponds to a family of at least four proteins (p50, p55, p75, and p85) differentially induced with biphasic kinetics during T cell activation. While p55 and p50 are closely related to the 50 kd DNA binding subunit of NF-kappa B, p75 and p85 exhibit DNA binding properties that distinguish them from this 50 kd polypeptide and its regulatory subunits I kappa B and p65. All four members of this kappa B-specific protein family are structurally related to the v-Rel oncoprotein and one, p85, appears identical to human c-Rel. v-Rel, but not nontransforming v-Rel mutants, binds to the kappa B enhancer and inhibits NF-kappa B-activated transcription from the IL-2 receptor alpha promoter and HIV-1 LTR. These findings suggest a Rel-related family of kappa B enhancer binding proteins and raise the possibility that the transforming activity of v-Rel is linked to its inhibitory action on cellular genes under NF-kappa B control.
Ballard
D W
DW
Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710.
Walker
W H
WH
Doerre
S
S
Sista
P
P
Molitor
J A
JA
Dixon
E P
EP
Peffer
N J
NJ
Hannink
M
M
Greene
W C
WC
D001483:Base Sequence; D015342:DNA Probes; D004268:DNA-Binding Proteins / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D005822:Genetic Vectors; D006367:HeLa Cells / Q000201:enzymology; D006801:Humans; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000502:physiology*; D015345:Oligonucleotide Probes; D020572:Oncogene Proteins v-rel; D009857:Oncogenes; D010449:Peptide Mapping; D010957:Plasmids; D014176:Protein Biosynthesis; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection
D015342:DNA Probes; D004268:DNA-Binding Proteins; D016328:NF-kappa B; D015345:Oligonucleotide Probes; D020572:Oncogene Proteins v-rel; D015682:Retroviridae Proteins, Oncogenic; D011505:Protein-Tyrosine Kinases