Isolation of anti-idiotypic antibodies by immunoaffinity chromatography on Affinichrom beads.
Anti-idiotypic antibodies are important regulators of the immune system but they are difficult to isolate and monitor. We have developed a technique for isolating specific auto-anti-idiotypic antibodies by high-performance immunoaffinity chromatography using immobilized autologous anti-tumor antibodies as the affinity ligand. The isolated anti-idiotypes demonstrated the ability to react with the original antitumor antibodies and inhibit their reactivity against autologous tumor cells. This technique can be used to monitor regulatory antibodies in cancer patients receiving immune modulation therapy.
Phillips
T M
TM
Immunochemistry Laboratory, George Washington University Medical Center, Washington, DC 20037.
Babashak
J V
JV
D000906:Antibodies / Q000302:isolation & purification*; D000912:Antibodies, Neoplasm / Q000032:analysis / Q000302:isolation & purification; D001426:Bacterial Proteins; D015153:Blotting, Western; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007120:Immunochemistry; D007457:Iodine Radioisotopes; D019809:Streptavidin; D014407:Tumor Cells, Cultured / Q000276:immunology
D000906:Antibodies; D000912:Antibodies, Neoplasm; D001426:Bacterial Proteins; D007457:Iodine Radioisotopes; D019809:Streptavidin