title: Colicin M is only bactericidal when provided from outside the cell.
abstract: The colicin M structural gene, cma, was subcloned in a vector which allowed temperature-inducible control of its expression. Induction of expression of cma in colicin M uptake proficient strains was lethal for the host cell when the colicin M immunity protein was not present. In liquid culture cells lysed, and no colonies were formed on solid media. These effects were not observed in mutants defective in the colicin receptor (FhuA) or uptake functions (TonB, TolM), nor in wild-type cells treated with trypsin prior to induction of cma expression. It was concluded that cytoplasmic colicin M is not toxic for the producing cell. To exert a lethal effect the colicin has to enter the cell from outside. Cells expressing cma released small amounts of colicin M.
authors.affiliation: Mikrobiologie II, Universität Tübingen, Federal Republic of Germany.
authors.forename: R E
authors.initials: RE
authors.lastname: Harkness
authors.forename: V
authors.initials: V
authors.lastname: Braun
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001433:Bacteriolysis; D003001:Cloning, Molecular; D003087:Colicins / Q000378:metabolism / Q000633:toxicity*; D003593:Cytoplasm / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic / Q000378:metabolism; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D003087:Colicins; D029968:Escherichia coli Proteins; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; C488856:cmI protein, E coli; C007104:colicin immunity proteins; C028593:colicin receptor, E coli