Thymidine incorporation by chick pineal glands as studied by pulse-labelling in vitro.
We have studied the pattern of variations in extent of thymidine incorporation during pulse-labelling in cultured chick pineal glands. During the first 24-30 h in culture the extent of pulse-labelling varied in parallel with the cycle in cumulative incorporation. Pulse-labelling was seen during the period of apparent arrest of cumulative incorporation. However, it was probably "masked" by the SD of assays of cumulative incorporation and represented a minor, but distinct, process of thymidine incorporation. The pattern of variation in extent of pulse-labelling was qualitatively consistent under a wide variety of conditions. Control of this pattern appeared to be different from that of the circadian rhythm in cumulative thymidine incorporation.
Wainwright
S D
SD
Biochemistry Department, Dalhousie University, Halifax, Nova Scotia, Canada.
Wainwright
L K
LK
D000818:Animals; D002645:Chickens; D002940:Circadian Rhythm; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D009924:Organ Culture Techniques; D010870:Pineal Gland / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism*; D014316:Tritium
D003470:Culture Media; D014316:Tritium; D004247:DNA; D013936:Thymidine