title: Studies on the extraction of different proteoglycan populations in bovine articular cartilage.
abstract: Large proteoglycan monomers and small dermatan sulfate proteoglycans were extracted from explants of bovine articular cartilage with increasing (0-4 M) concentrations of guanidinium chloride (GuHCl). The first extractions were followed by a second extraction with 4 M GuHCl. The amount of proteoglycans extracted in the first buffer depended on the GuHCl concentration. At low concentrations of GuHCl, a relatively high amount of small proteoglycans was obtained. Fifty percent of the small proteoglycans was extracted in buffer with 0.85 M GuHCl, while 2.0-2.2 M GuHCl was needed to extract half of the large proteoglycans. Immediately after synthesis, 35S-labeled large proteoglycans were extracted much easier (50% at 1.4 M GuHCl), and those extracted at low concentrations of GuHCl were less capable of aggregation with hyaluronic acid. After 7 days of 'chase' these differences between endogenous and 35S-labeled proteoglycans had disappeared.
authors.affiliation: Jan van Breemen Instituut, Amsterdam.
authors.forename: H S
authors.initials: HS
authors.lastname: Brand
authors.forename: G H
authors.initials: GH
authors.lastname: Korver
authors.forename: R J
authors.initials: RJ
authors.lastname: van de Stadt
authors.forename: G P
authors.initials: GP
authors.lastname: van Kampen
authors.forename: J K
authors.initials: JK
authors.lastname: van der Korst
mesh_terms: D000818:Animals; D002021:Buffers; D002358:Cartilage, Articular / Q000737:chemistry*; D002417:Cattle; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011509:Proteoglycans / Q000302:isolation & purification*
chemical_list: D002021:Buffers; D011509:Proteoglycans