Purification of immunomodulatory factors in human peripheral blood leukocytes.
Procedure is described for purifying low-molecular-weight factors with antigen-aspecific properties from a dialysate of human leukocyte extract. It includes gel chromatography on Sephadex G-25 and G-15, ion-exchange chromatography, reversed-phase high-performance liquid chromatography (HPLC) on a C18 hydrophobic column and gel permeation HPLC. The immunosuppressive factor (mol.wt. 800-1000) was purified to near homogeneity. It is probably of peptidic nature, although it is pronase resistant. The enhancer factor (mol.wt. 300-600) is eluted from chromatographic columns together with a hypoxanthine-like substance. Nevertheless, the biological activity cannot be attributed to the purine derivative. Identification of this amplifier activity is still lacking.
Montecucchi
P C
PC
Sclavo SpA, DE.BI, Cassina dé Pecchi, Italy.
Caldarini
R
R
Sansó
M
M
Bertolaso
A M
AM
Rosso di San Secondo
V E
VE
Piccolo
G
G
Sirchia
G
G
D000276:Adjuvants, Immunologic / Q000032:analysis / Q000302:isolation & purification*; D000596:Amino Acids / Q000032:analysis; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006801:Humans; D007166:Immunosuppressive Agents / Q000032:analysis / Q000302:isolation & purification*; D066298:In Vitro Techniques; D007962:Leukocytes / Q000737:chemistry* / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D008970:Molecular Weight; D010835:Phytohemagglutinins / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet
D000276:Adjuvants, Immunologic; D000596:Amino Acids; D007166:Immunosuppressive Agents; D010835:Phytohemagglutinins