Competitive erythroimmunoassay for detecting Clostridium perfringens type A enterotoxin in stool specimens.
A competitive erythroimmunoassay (ERIA) is described for Clostridium perfringens enterotoxin (CPE) detection in stools. This technique uses sheep red blood cells sensitized by CPE and an anti-CPE-antibody-coated plate in which the results are read by eye. ERIA is simple, rapid, economic and more sensitive (2 ng/ml) than the enzyme-linked immunosorbent assay used for evaluation. ERIA is suitable for CPE detection in stool samples protected with phenylmethylsulphonylfluoride.
Germani
Y
Y
Institut Pasteur de Nouméa, Laboratoire des Bactéries entéropathogénes, Nouméa.
Popoff
M R
MR
Bégaud
E
E
Guesdon
J L
JL
D003015:Clostridium Infections / Q000175:diagnosis*; D003016:Clostridium perfringens / Q000302:isolation & purification*; D003967:Diarrhea / Q000382:microbiology*; D004768:Enterotoxins; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007118:Immunoassay; D066298:In Vitro Techniques
D004768:Enterotoxins