Phosphorylation of ornithine decarboxylase by casein kinase II from RAW264 cells.
Casein kinase II and ornithine decarboxylase were purified from a virally-transformed macrophage-like cell line, RAW264. The addition of casein kinase II to a reaction mixture containing [tau-32P]GTP, Mg++, and ornithine decarboxylase led to the phosphorylation of a 55,000 dalton protein band in the purified preparation of ornithine decarboxylase. Stoichiometric estimates indicated that casein kinase II incorporated 0.15 mole of phosphate per mole of ornithine decarboxylase, which was increased to 0.3 mole/per mole in the presence of spermine. The apparent Km and Vmax values for the casein kinase II-mediated phosphorylation of ornithine decarboxylase were 0.36 microM and 62.5 nmol/min./mg kinase. The addition of spermine to the reaction did not alter the Km but increased the Vmax to 100 nmol/min./mg kinase. The phosphorylation of ornithine decarboxylase by casein kinase II affected neither the rate of maximal ornithine decarboxylase activity nor the affinity of the enzyme for ornithine.
Tipnis
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UR
Department of Pathology, University of Texas Medical School, Galveston 77550.
Haddox
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MK
D000596:Amino Acids / Q000032:analysis; D047388:Casein Kinases; D002460:Cell Line; D007700:Kinetics; D008264:Macrophages / Q000201:enzymology*; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D010766:Phosphorylation; D011233:Precipitin Tests; D011494:Protein Kinases / Q000378:metabolism*
D000596:Amino Acids; D011494:Protein Kinases; D047388:Casein Kinases; D009955:Ornithine Decarboxylase