title: Adrenal P-450scc catalyzes deoxycorticosterone 6 beta-hydroxylase reaction.
abstract: Purified bovine P-450scc, the cholesterol side-chain cleaving P-450 in adrenal cortex mitochondria, was found to catalyze a deoxycorticosterone 6 beta-hydroxylase reaction. A turnover number (moles of product formed/min/mol of P-450) of 12 was found similar to that for cholesterol side chain cleavage activity. Conversion was dose-dependent in terms of P-450scc and no reaction took place when any one of the required electron donating components such as NADPH, NADPH-adrenodoxin reductase, or adrenodoxin was omitted. These results confirm and extend earlier observations that 21-hydroxypregnenolone is transformed into both deoxycorticosterone and 6 beta-hydroxydeoxycorticosterone by incubation of adrenal gland slices.
authors.affiliation: Department of Chemistry, Faculty of Science, Kanazawa University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Mochizuki
authors.forename: K
authors.initials: K
authors.lastname: Suhara
authors.forename: M
authors.initials: M
authors.lastname: Katagiri
mesh_terms: D000302:Adrenal Cortex / Q000201:enzymology*; D000325:Adrenodoxin / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003900:Desoxycorticosterone / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D009249:NADP / Q000378:metabolism
chemical_list: D000325:Adrenodoxin; D003900:Desoxycorticosterone; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases