An algorithm for the computer calculation of the coefficients of a polynomial that allows determination of isoelectric points of proteins and other macromolecules.
The isoelectric point (pI) of a macromolecule containing any number of acid-base residues can be expressed as a polynomial whose coefficients are related to both the number of acid-base residues present in the molecule and their K values. Polynomials of degree higher than 5 are too complicated to be solved manually. An algorithm is here presented which allows, with few sentences written in BASIC, the calculation of the polynomial coefficients. With this knowledge and using standard polynomial solving programs, the isoelectric point of the macromolecule can be calculated.
Ribeiro
J M
JM
Departamento de Bioquímica, Facultad de Medicina, Universidad Autónoma, Madrid.
Sillero
A
A
D000465:Algorithms; D007526:Isoelectric Point; D046911:Macromolecular Substances; D008432:Mathematical Computing; D008838:Microcomputers; D011506:Proteins / Q000737:chemistry*; D012984:Software; D012985:Software Design
D046911:Macromolecular Substances; D011506:Proteins