[Development and application of enzyme-linked immunosorbent assay using monoclonal antibody against a hemolysin (Vp-TRH) of Vibrio paraheamolyticus--evidence that Vp-TRH producing-Kanagawa phenomenon-negative V. parahaemolyticus is a human pathogen].
Although it has been well established that Kanagawa phenomenon-positive Vibrio parahaemolyticus is a human enteropathogen, the Kanagawa phenomenon-negative one has been considered to be probably not pathogenic. We have found, however, an outbreak of gastroenteritis due to Kanagawa phenomenon-negative V. parahaemolyticus which produces a new toxin (Vp-TRH) resembling to Vp-TDH, a responsible toxin of Kanagawa phenomenon. In this study, we developed monoclonal antibodies against Vp-TRH which were used for development of an enzyme-linked immunosorbent assay (ELISA) for specifically detecting Vp-TRH. The ELISA was applied for analysis of production of Vp-TRH by various isolates of V. parahaemolyticus and we found that Vp-TRH-producing strains were derived mostly from human diarrheal stool, and not from the environment or sea foods. The results of the rabbit ileal loop test showed that Vp-TRH-producing (Kanagawa phenomenon-negative) strains, as well as Vp-TDH-producing (Kanagawa phenomenon-positive) strains could induce fluid accumulation. These results indicate the possibility that Vp-TRH-producing Kanagawa phenomenon-negative V. parahaemolyticus is a human enteropathogen.
Honda
T
T
Research Institute for Microbial Diseases, Osaka University.
Honda
S
S
Ni
Y X
YX
Miwatani
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006460:Hemolysin Proteins / Q000032:analysis* / Q000276:immunology; D006461:Hemolysis; D006801:Humans; D011817:Rabbits; D014736:Vibrio parahaemolyticus / Q000378:metabolism* / Q000472:pathogenicity
D000911:Antibodies, Monoclonal; D006460:Hemolysin Proteins