[The preparation of an IgG diagnostic agent based on stained polyacrolein latexes for use in the latex agglutination reaction].
IgG diagnosticum for measuring the concentration of 131I-labeled IgG antibodies to enteric antigen beta 1MA by the latex agglutination inhibition (LAI) test has been prepared on the basis of polyacrolein latexes. A method for the titration of anti-IgG antibodies with the use of the above diagnosticum has been developed, based on the late, agglutination (LA) test. The optimum conditions for the microtitration variant of the LA and LAI tests have been defined. High sensitivity, specificity and simplicity of analysis with the use of latex IgG diagnosticum have been demonstrated. The newly developed methods have been successfully used in laboratory trials of a new diagnostic radiopharmaceuticals for the assay of 131I-labeled antibodies in this preparation and for the detection of side effects of immunization on the recipients.
Aleksander
S K
SK
Lukin
Iu V
IuV
Fidler
L M
LM
Zubov
V P
VP
Terent'eva
E A
EA
Mart'ianov
B M
BM
D000171:Acrolein; D000818:Animals; D000941:Antigens / Q000276:immunology; D004285:Dogs; D005069:Evaluation Studies as Topic; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007840:Latex; D007841:Latex Fixation Tests / Q000295:instrumentation / Q000379:methods*; D008470:Meconium / Q000276:immunology; D051379:Mice; D010316:Particle Size; D011108:Polymers; D011817:Rabbits; D051381:Rats
D000941:Antigens; D007074:Immunoglobulin G; D007840:Latex; D011108:Polymers; C029975:polyacrolein; D000171:Acrolein
Prigotovlenie IgG-diagnostikuma na osnove okrashennykh poliakroleinovykh lateksov dlia primeneniia v reaktsii lateksnoĭ aggliutinatsii.