Effect of alcohol sulfate, linear alkylbenzene sulfonate and natural soap on the development of fertilized eggs of the mouse in vitro.
Eggs from B6 x C3F1 female mice, which were fertilized in vitro with sperm from C3 x 101F1 male mice, were treated with synthetic surfactants, alcohol sulfate (AS) and linear alkylbenzene sulfonate (LAS), and natural soap for 1 h at the pronucleus stage, and then cultivated for 5 days. Eggs treated with AS or LAS at concentrations of less than 0.025% developed to the blastocyst stage as well as the untreated ones. At concentrations of AS or LAS higher than 0.03% no egg developed beyond the 1-cell stage. There appeared to be a threshold concentration between 0.025% and 0.03% of AS or LAS on the development of the mouse egg. However, natural soap had no effect on the development of the mouse egg up to 0.05%. When AS or LAS was applied to the culture medium throughout the cultivation of fertilized eggs for 5 days, there also appeared to be a threshold concentration between 0.01% and 0.025%, but not in the case of natural soap. The results provide additional support to our previous observations that AS and LAS can interrupt mouse pregnancy by killing fertilized eggs.
Ishii
Y
Y
Department of Radiation Biology, Faculty of Medicine, Osaka University, Japan.
Samejima
Y
Y
Saji
F
F
Nomura
T
T
D000438:Alcohols / Q000633:toxicity*; D000473:Alkanes / Q000633:toxicity; D000818:Animals; D001557:Benzenesulfonates / Q000633:toxicity*; D001755:Blastocyst / Q000187:drug effects; D004622:Embryo, Mammalian / Q000187:drug effects*; D005314:Embryonic and Fetal Development; D005260:Female; D008297:Male; D051379:Mice; D012915:Soaps / Q000633:toxicity*; D013464:Sulfuric Acids / Q000633:toxicity; D013501:Surface-Active Agents / Q000633:toxicity*
D000438:Alcohols; D000473:Alkanes; D001557:Benzenesulfonates; D012915:Soaps; D013464:Sulfuric Acids; D013501:Surface-Active Agents