Spiroadamantane dioxetane substrates--stable labels for luminescence-enhanced enzyme immunoassays.
This short communication describes the use of spiroadamantane-1,2 dioxetane substrates for alkaline phosphatase and beta-D-galactosidase in the development of luminescence-enhanced enzyme immunoassays. Until the present work, only peroxidase-luminol/peroxidase systems had been used in such assays. The light reaction kinetics were studied from the time of initiation of the light reaction up to 2 days later. The methods is illustrated by the determination of myeloperoxidase, based on microtitre plate technology, coupled with a microtitre plate luminometer which needs no injector. The alkaline phosphatase and peroxidase systems were compared.
Wood
W G
WG
Klinische Laboratorien, Medizinische Universität zu Lübeck.
D000218:Adamantane / Q000031:analogs & derivatives*; D000469:Alkaline Phosphatase; D007124:Immunoenzyme Techniques; D007462:Iodobenzenes; D008163:Luminescent Measurements; D008165:Luminol; D010544:Peroxidases; D010545:Peroxides
D007462:Iodobenzenes; D010545:Peroxides; C061523:3-(2'-spiroadamantane)-4-methoxy-4-(3''-phosphoryloxy)phenyl-1,2-dioxetane; C050181:4-iodophenol; D008165:Luminol; D010544:Peroxidases; D000469:Alkaline Phosphatase; D000218:Adamantane