Change in hexose distribution volume and fractional utilization of [18F]-2-deoxy-2-fluoro-D-glucose in brain during acute hypoglycemia in humans.
We used positron emission tomography (PET) to study the effects of mild hypoglycemia on cerebral glucose uptake and metabolism. Nine healthy men were studied under basal saline-infusion conditions, and during euglycemic and hypoglycemic clamp studies. Insulin was infused at the same rate (1 mU.kg-1.min-1) in both clamp studies. In euglycemic clamp studies, glucose was infused at a rate sufficient to maintain the basal plasma glucose concentration, whereas in hypoglycemic clamp studies, the glucose infusion rate was reduced to maintain the plasma glucose at 3.1 mM. Each study lasted 3 h and included a 30-min baseline period and a subsequent 150-min period in which insulin or glucose was administered. Blood samples for measurement of insulin, glucose, cortisol, growth hormone, and glucagon were obtained at 20- to 30-min intervals. A bolus injection of 5-10 mCi [18F]-2-deoxy-2-fluoro-D-glucose (2-DFG) was administered 120 min after initiation of the study, and plasma radioactivity and dynamic PET scans were obtained at frequent intervals for the remaining 40-60 min of the study. Cerebral regions of interest were defined, and concentrations of radioactivity were calculated and used in the three-compartment model of 2-DFG distribution described by Sokoloff. Glucose levels were similar during saline-infusion (4.9 +/- 0.1 mM) and euglycemic clamp (4.8 +/- 0.1 mM) studies, whereas the desired degree of mild hypoglycemia was achieved during the hypoglycemic clamp study (3.1 +/- 0.1 mM, P less than 0.05). The insulin level during saline infusion was 41 +/- 7 pM.(ABSTRACT TRUNCATED AT 250 WORDS)
Shapiro
E T
ET
University of Chicago, Department of Medicine, Pritzer School of Medicine, Chicago, Illinois 60637.
Cooper
M
M
Chen
C T
CT
Given
B D
BD
Polonsky
K S
KS
D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D003847:Deoxyglucose / Q000031:analogs & derivatives* / Q000378:metabolism / Q000493:pharmacokinetics; D005462:Fluorine Radioisotopes; D019788:Fluorodeoxyglucose F18; D005934:Glucagon / Q000097:blood; D015309:Glucose Clamp Technique; D013006:Growth Hormone / Q000097:blood; D006601:Hexoses / Q000032:analysis*; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D007003:Hypoglycemia / Q000378:metabolism*; D007328:Insulin / Q000097:blood; D008297:Male; D014018:Tissue Distribution; D014055:Tomography, Emission-Computed
D001786:Blood Glucose; D005462:Fluorine Radioisotopes; D006601:Hexoses; D007328:Insulin; D019788:Fluorodeoxyglucose F18; D013006:Growth Hormone; D005934:Glucagon; D003847:Deoxyglucose; D006854:Hydrocortisone