[Modeling of damage to cell membranes during photodynamic therapy: photosensitization of planar lipid bilayers by hematoporphyrin dimethyl ether].
The variations of electrical conductance of planar bilayer lipid membranes (BLM) sensitized by hematoporphyrin dimethyl ether under visible light illumination were studied. The conductance of BLM does not change for some period after switching on the light, then an increase in the conductance starts and the membrane breaks. This "induction" time does not depend on addition of azide or ferricyanide to the solution, on addition of BHT to the lipid and on substitution of air for argon in the cell. The induction time for any new BLM, formed in the same cell immediately after the previous membrane was broken, is shorter. The variation of BLM boundary potentials during induction time was not observed. The results obtained suggest that the photodamage of BLM sensitized by HPD leads to accumulation of uncharged reaction products and oxygen does not take part in this process.
Stozhkova
I N
IN
Mirskiĭ
V M
VM
D002462:Cell Membrane / Q000187:drug effects*; D006414:Hematoporphyrin Photoradiation; D006415:Hematoporphyrins / Q000494:pharmacology; D006801:Humans; D008051:Lipid Bilayers; D008563:Membrane Lipids; D008954:Models, Biological
D006415:Hematoporphyrins; D008051:Lipid Bilayers; D008563:Membrane Lipids
Modelirovanie povrezhdeniia kletochnykh membran pri fotodinamicheskoĭ terapii: fotosensibilizatsiia ploskogo lipidnogo bisloia dimetilovym efirom gematoporfirina.