Enhanced expression of group II phospholipase A2 gene in the tissues of endotoxin shock rats and its suppression by glucocorticoid.
We studied the regulation of group II phospholipase A2 (PLA2-II) gene in vivo, using endotoxin shock rat as a model for systemic inflammation. Administration of endotoxin into rats increased PLA2 activity in the plasma, as described by Vadas and Hay, using endotoxin-challenged rabbit. Specific absorption of this activity by anti-PLA2-II antibody indicated that the released PLA2 was PLA2-II. The levels of PLA2-II mRNA were elevated in the aorta, spleen, lung, and thymus but not in the liver and kidney. The tissues with high PLA2-II mRNA contents released a greater amount of PLA2-II than the tissues of control rats. These results suggest that in endotoxin shock rats, PLA2-II is synthesized de novo in the above tissues and released into circulation. Furthermore, our present study demonstrates that glucocorticoid suppresses the enhanced expression of the PLA2-II gene in the tissues of endotoxin shock rats.
Nakano
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Shionogi Research Laboratories, Shionogi & Co. Ltd., Osaka, Japan.
Arita
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D000818:Animals; D001011:Aorta / Q000201:enzymology; D003907:Dexamethasone / Q000494:pharmacology*; D004731:Endotoxins; D004926:Escherichia coli; D015870:Gene Expression / Q000187:drug effects; D008168:Lung / Q000201:enzymology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000201:enzymology; D009928:Organ Specificity; D010741:Phospholipases A / Q000235:genetics*; D054467:Phospholipases A2; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012772:Shock, Septic / Q000201:enzymology*; D013489:Suppression, Genetic; D013950:Thymus Gland / Q000201:enzymology
D004731:Endotoxins; D012333:RNA, Messenger; D003907:Dexamethasone; D010741:Phospholipases A; D054467:Phospholipases A2