title: Blockage of cell wall receptors for yeast killer toxin KT28 with antimannoprotein antibodies.
abstract: Binding of yeast killer toxin KT28 to its primary cell wall receptor was specifically blocked with polyclonal antimannoprotein antibodies which masked all toxin-binding sites on the surface of sensitive yeast cells. By indirect immunofluorescence, it was shown that KT28 binds to the cell wall mannoprotein and that the toxin resistance of mannoprotein mutants (mnn) of Saccharomyces cerevisiae was due to a lack of killer toxin-binding sites within the yeast cell wall. Structural analysis of acetylated mannoprotein from KT28-resistant mutant strains identified the outer mannotriose side chains as the actual killer toxin-binding domains.
authors.affiliation: Institut für Mikrobiologie und Weinforschung, Johannes Gutenberg-Universität Mainz, Federal Republic of Germany.
authors.forename: M J
authors.initials: MJ
authors.lastname: Schmitt
authors.forename: F
authors.initials: F
authors.lastname: Radler
mesh_terms: D000906:Antibodies / Q000276:immunology*; D002473:Cell Wall / Q000187:drug effects* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D055646:Killer Factors, Yeast; D008562:Membrane Glycoproteins / Q000276:immunology*; D009183:Mycotoxins / Q000276:immunology / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D000906:Antibodies; D055646:Killer Factors, Yeast; D008562:Membrane Glycoproteins; D009183:Mycotoxins; D029701:Saccharomyces cerevisiae Proteins; C025363:mannoproteins