Affinity chromatography of DNA fragments and P-modified oligonucleotides.
The wide possibilities for use of affinity chromatography are demonstrated by two examples: (i) isolation of a single-stranded fragment of the tick-borne encephalitis virus DNA (302-mer) and an oligonucleotide (34 bases) from reaction mixtures and (ii) fractionation of mixtures of diastereoisomers of octathymidylates with modified internucleotide phosphates. All affinity sorbents are constructed by the covalent attachment of the oligonucleotides to solid supports and can be used repeatedly.
Zarytova
V F
VF
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences.
Shishkina
I G
IG
D001483:Base Sequence; D002021:Buffers; D002846:Chromatography, Affinity; D004279:DNA, Viral / Q000032:analysis*; D004669:Encephalitis Viruses, Tick-Borne / Q000032:analysis; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000032:analysis* / Q000138:chemical synthesis; D013237:Stereoisomerism
D002021:Buffers; D004279:DNA, Viral; D009841:Oligonucleotides