title: Penetration of topically applied betamethasone sodium phosphate into human aqueous humour.
abstract: Gas Chromatography combined with Negative Chemical Ionisation Mass Spectrometry (GCMS) was used to determine the absorption of topically applied beta-methasone sodium phosphate into the aqueous humour of human subjects undergoing routine intraocular surgery. The Betamethasone concentration was greatest in the interval 91-120 minutes following topical administration (mean peak concentration = 7.7 ng/ml). At twelve hours post instillation the mean concentration of Betamethasone was 2.5 ng/ml and detectable levels were recorded in the aqueous humour 24 hours after application (mean concentration 0.4 ng/ml).
authors.affiliation: Department of Pharmacy, University of Strathclyde, Glasgow.
authors.forename: D G
authors.initials: DG
authors.lastname: Watson
authors.forename: C N
authors.initials: CN
authors.lastname: McGhee
authors.forename: J M
authors.initials: JM
authors.lastname: Midgley
authors.forename: G N
authors.initials: GN
authors.lastname: Dutton
authors.forename: M J
authors.initials: MJ
authors.lastname: Noble
mesh_terms: D000287:Administration, Topical; D000368:Aged; D000369:Aged, 80 and over; D001082:Aqueous Humor / Q000187:drug effects / Q000378:metabolism*; D001623:Betamethasone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D002387:Cataract Extraction; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008875:Middle Aged; D013997:Time Factors
chemical_list: C028994:betamethasone sodium phosphate; D001623:Betamethasone