Electrophysiological properties of subpopulations of rat dorsal root ganglion neurons in vitro.
Intracellular recordings were made from rat dorsal root ganglion neurons in vitro. Action potentials with an inflection on the falling phase occurred in all cells conducting up to 5.2 m/s and in a proportion of faster conducting cells which decreased with increasing conduction velocity, until no cells conducting faster than 31 m/s had an inflection. Overall, all C-cells (less than 1.3 m/s), 61% of A delta-cells (1.3-12 m/s) and 23% of A alpha/beta-cells (greater than 12 m/s) had inflections. A-cells with inflections were found to be electrophysiologically distinct from those without as they differed in the mean and distribution of every action potential and afterhyperpolarization parameter measured. C-cells differed from all A-cell groups, but the means and distributions of most parameters were closer to those of A-cells with inflections than of A-cells without. In addition, all A- and C-cell action potentials with inflections were tetrodotoxin resistant, while all those without were sensitive. The only parameters whose means differed between A alpha/beta- and A delta-neurons were ones which correlated with conduction velocity (action potential duration, overshoot and maximum rate of rise and fall). The response pattern to prolonged current injection did not correlate with conduction velocity, but slightly more A-cells with inflections were single firing. A-cells with long afterhyperpolarizations always fired singly, while those with shorter durations fired singly or multiply. Somatic following frequency was most strongly limited by long afterhyperpolarization duration; it was also slightly lower in A delta- than in A alpha/beta-cells, and lower in A-cells with inflections than in those without. Fibre following frequencies were highest in the fastest conduction neurons.
Waddell
P J
PJ
Physiology Department, Medical School, Bristol, U.K.
Lawson
S N
SN
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D004351:Drug Resistance; D004594:Electrophysiology; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology / Q000502:physiology*; D008564:Membrane Potentials; D009431:Neural Conduction; D009474:Neurons / Q000502:physiology*; D012964:Sodium / Q000502:physiology; D013779:Tetrodotoxin / Q000494:pharmacology; D013997:Time Factors
D013779:Tetrodotoxin; D012964:Sodium