Behavior of the hybrid plasmid pNSW301 in Zymomonas mobilis grown in continuous culture.
The stability of the plasmid pNSW301 which was formed by cointegration of the Inc W R plasmid Sa and the 14.5-kb pNSW1 plasmid of Zymomonas mobilis ZM6100 was investigated in ZM6100(pNSW301) grown in continuous culture without antibiotic selection. The cointegrate plasmid, pNSW301, was found to be structurally unstable and a total reduction in the size of pNSW301 of approximately 21 kb occurred during growth in continuous culture. Following a systematic study, a number of deletion derivatives of pNSW301 were isolated and used to transform Escherichia coli HB101, with the exception of pNSW312. The plasmid pNSW312 was 100% stable in ZM6100(pNSW312) in continuous culture but was unable to replicate in E. coli.
Strzelecki
A T
AT
Department of Biotechnology, University of New South Wales, Kensington, Australia.
Goodman
A E
AE
Cail
R G
RG
Rogers
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PL
D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004352:Drug Resistance, Microbial / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D006089:Gram-Negative Anaerobic Bacteria / Q000235:genetics* / Q000254:growth & development; D009693:Nucleic Acid Hybridization; D010957:Plasmids
D015342:DNA Probes; D004269:DNA, Bacterial