Chromatography and generation of specific antisera to synthetic peptides from a protective Boophilus microplus antigen.
Four oligopeptides corresponding to predicted antigenic regions of the protective Bm86 glycoprotein of the cattle tick Boophilus microplus were synthesized and purified. Three were conjugated to carrier proteins and antisera raised in rabbits and cows. All elicited antipeptide antibodies that recognized Bm86 and recombinant derived products in Western blots; however, only one produced antiserum capable of recognizing native Bm86 in an indirect immunofluorescence assay. Ticks fed in vitro on this antiserum showed no obvious gut damage.
Sharp
P J
PJ
Biotechnology Australia Pty. Ltd., Roseville, N.S.W.
McInerney
B V
BV
Smith
D R
DR
Turnbull
I F
IF
Kemp
D H
DH
Rand
K N
KN
Cobon
G S
GS
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology*; D001233:Aspergillus nidulans / Q000276:immunology; D015153:Blotting, Western; D002352:Carrier Proteins / Q000032:analysis / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D004220:Disulfides; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D008715:Methionine / Q000031:analogs & derivatives; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000138:chemical synthesis / Q000276:immunology / Q000302:isolation & purification*; D010084:Oxidation-Reduction; D011487:Protein Conformation; D011817:Rabbits / Q000276:immunology; D013987:Ticks / Q000276:immunology*
D000596:Amino Acids; D000906:Antibodies; D000941:Antigens; D002352:Carrier Proteins; D004220:Disulfides; D009842:Oligopeptides; D008715:Methionine; C013111:methionine sulfoxide