Double monoclonal time-resolved immunofluorometric assay of beta 2-microglobulin in serum.
Previously reported immunochemical assays of beta 2-microglobulin (beta 2m) have usually been based on polyclonal antisera. We have developed a "sandwich"-type time-resolved immunofluorometric assay (TR-IFMA) for beta 2m in serum, based on two monoclonal antibodies against human beta 2m. Microtiter wells are coated with the capture antibody, and the tracer antibody is labeled with a europium chelate. In a simple and fast assay procedure, prediluted serum samples are incubated with the tracer for 1 h in the microtiter wells, after which the wells are washed and the fluorescence of Eu is measured. The mean analytical recovery was 101.8% and results by TR-IFMA showed a good linear correlation with those by an established radioimmunoassay. The analytical range of TR-IFMA is large and well suited for clinical purposes.
Tienhaara
A
A
Central Laboratory, Turku University Central Hospital, Finland.
Eskola
J U
JU
Näntö
V
V
D000328:Adult; D000368:Aged; D000818:Animals; D002624:Chemistry, Clinical / Q000592:standards; D005260:Female; D015200:Fluoroimmunoassay / Q000379:methods; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D012015:Reference Standards; D001613:beta 2-Microglobulin / Q000032:analysis*
D001613:beta 2-Microglobulin