title: Amino acid sequence of CAP37, a human neutrophil granule-derived antibacterial and monocyte-specific chemotactic glycoprotein structurally similar to neutrophil elastase.
abstract: We report the amino acid sequence of CAP37, a human neutrophil granule protein with antibacterial and monocyte-specific chemotactic activity. CAP37 is a single-chain protein consisting of 222 amino acid residues. It has three N-glycosylation sites, at Asn residues 100, 114 and 145. Some species of CAP37 are glycosylated at all three sites; some at Asn-114 alone, others at Asn-114 and Asn-110 or Asn-145. CAP37 has 45% sequence identity to human neutrophil elastase, and 30-37% identity to several other granule serine proteinases. Despite these similarities, CAP37 is not a serine proteinase because the active site residues serine and histidine are replaced.
authors.affiliation: Microchemical Facility, Winship Cancer Center, Atlanta, GA.
authors.forename: J
authors.initials: J
authors.lastname: Pohl
authors.forename: H A
authors.initials: HA
authors.lastname: Pereira
authors.forename: N M
authors.initials: NM
authors.lastname: Martin
authors.forename: J K
authors.initials: JK
authors.lastname: Spitznagel
mesh_terms: D000595:Amino Acid Sequence; D023181:Antimicrobial Cationic Peptides; D001770:Blood Bactericidal Activity; D001798:Blood Proteins / Q000737:chemistry*; D002352:Carrier Proteins; D002630:Chemotactic Factors / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004220:Disulfides; D010450:Endopeptidases / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry*; D006031:Glycosylation; D019272:Leukocyte Elastase; D008666:Metalloendopeptidases; D008969:Molecular Sequence Data; D010196:Pancreatic Elastase / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry; D012689:Sequence Homology, Nucleic Acid; D014357:Trypsin / Q000378:metabolism
chemical_list: C062991:AZU1 protein, human; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D002352:Carrier Proteins; D002630:Chemotactic Factors; D004220:Disulfides; D006023:Glycoproteins; D010446:Peptide Fragments; D010450:Endopeptidases; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase; D014357:Trypsin; D008666:Metalloendopeptidases; C058921:endoproteinase Asp-N