title: Cresyl violet: a red fluorescent Nissl stain.
abstract: Cresyl violet is widely used by neurobiologists to visualize Nissl substance in bright-field microscopy. Here we describe a method for using this dye as a red fluorescent Nissl stain. Unlike the bright-field staining technique, fluorescent cresyl is compatible with other fluorescent dyes and tracers, such as fluorescein, Fluoro-Gold and Fast Blue. The procedure requires only minor modifications of routine bright-field cresyl staining, the most significant being dilution of the stain. Thus, fluorescent red cresyl violet is simple to implement and may be of general use in fluorescence microscopy.
authors.affiliation: Rockefeller University, New York, NY 10021.
authors.forename: A
authors.initials: A
authors.lastname: Alvarez-Buylla
authors.forename: C Y
authors.initials: CY
authors.lastname: Ling
authors.forename: J R
authors.initials: JR
authors.lastname: Kirn
mesh_terms: D000818:Animals; D048588:Benzoxazines; D001717:Birds; D005456:Fluorescent Dyes; D009562:Nissl Bodies / Q000648:ultrastructure*; D010078:Oxazines; D014466:Ultraviolet Rays
chemical_list: D048588:Benzoxazines; D005456:Fluorescent Dyes; D010078:Oxazines; C028911:cresyl violet