title: Changes in the DNA polymerase beta gene expression during development of lung, brain, and testis suggest an involvement of the enzyme in DNA recombination.
abstract: Changes in the expression pattern of DNA polymerase beta gene during rat lung, brain, and testis development have been investigated. A decrease in the level of beta-pol mRNA was observed during postnatal development of lung and brain. By contrast, an almost 20-fold increase in the level of beta-pol mRNA was observed during spermatogenesis. For most adult rat tissues the abundance of beta-pol mRNA was low compared with that of beta-actin mRNA. Northern blot analysis revealed four distinct transcripts hybridizing to beta-pol probes. At least two of them, 1.4 kb and 4.0 kb, were products of a beta-polymerase gene. The changes in the expression pattern during lung and brain development, and during spermatogenesis, suggest involvement of DNA polymerase beta in gap-filling DNA synthesis during recombination.
authors.affiliation: Department of Cell Biology and Experimental Therapy, Cancer Center-Institute, Warsaw, Poland.
authors.forename: R
authors.initials: R
authors.lastname: Nowak
authors.forename: M
authors.initials: M
authors.lastname: Woszczynski
authors.forename: J A
authors.initials: JA
authors.lastname: Siedlecki
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D001921:Brain / Q000201:enzymology / Q000254:growth & development*; D004247:DNA / Q000096:biosynthesis; D004256:DNA Polymerase I / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression / Q000235:genetics*; D008168:Lung / Q000201:enzymology / Q000254:growth & development*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D011995:Recombination, Genetic; D013091:Spermatogenesis / Q000235:genetics; D013737:Testis / Q000201:enzymology / Q000254:growth & development*
chemical_list: D012333:RNA, Messenger; D004247:DNA; D004256:DNA Polymerase I