Recombinant human activin-A promotes proliferation of human luteinized preovulatory granulosa cells in vitro.
Ovarian granulosa cells synthesize and secrete activin, a member of the transforming growth factor-beta (TGF-beta) peptide family, during the follicular phase of the menstrual cycle. We examined the growth-promoting activity of human recombinant activin-A on human luteinized preovulatory granulosa cells obtained from women undergoing in vitro fertilization. Activin-A induced proliferation of granulosa cells on day 5 of culture in a dose-dependent manner. Maximal effects were seen at concentrations greater than or equal to 100 ng/mL with an ED50 of 15 ng/mL. We suggest that activin-A is able to modulate growth of ovarian granulosa cells.
Rabinovici
J
J
Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco 94143.
Spencer
S J
SJ
Jaffe
R B
RB
D028341:Activins; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D005307:Fertilization in Vitro; D006107:Granulosa Cells / Q000187:drug effects*; D006801:Humans; D007265:Inhibins / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology
D011994:Recombinant Proteins; D028341:Activins; D007265:Inhibins