Determination of influenza virus proteins required for genome replication.
An artificial vaccinia virus vector-driven replication system for influenza virus RNA has been developed. In this system, a synthetic NS-like gene is replicated and expressed by influenza virus proteins supplied through infection with vaccinia virus recombinant vectors. The minimum subset of influenza virus proteins needed for specific replication and expression of the viral ribonucleoprotein was found to be the three polymerase proteins (PB1, PB2, and PA) and the nucleoprotein.
Huang
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TS
Department of Microbiology, Mt. Sinai School of Medicine, New York, New York 10029-6574.
Palese
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Krystal
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D003001:Cloning, Molecular; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D009980:Influenza A virus / Q000235:genetics* / Q000254:growth & development; D009024:Morphogenesis; D012367:RNA, Viral / Q000235:genetics; D011994:Recombinant Proteins; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics; D014779:Virus Replication
D012367:RNA, Viral; D011994:Recombinant Proteins; D014764:Viral Proteins