Heterogeneity of Nef proteins in cells infected with human immunodeficiency virus type 1.
Human T-lymphocytic cell line H9 infected with the HTLV-IIIB isolate of human immunodeficiency virus type 1 (HIV-1) synthesizes two forms of the Nef protein (p25 and p27) that differ both in molecular weight and charge. Different subpopulations of viruses were isolated from the HTLV-IIIB stock which induce expression of only p25 or p27. Cells infected with HIV-1 derived from the HXB3 clone of the HTLV-IIIB isolate made only the p25 species, whereas the 8E5/LAV cell line which harbors a single defective LAV provirus produces only the p27 species. These findings are consistent with the notion that the HTLV-IIIB isolate consists of at least two distinct variants with different nef genes, one specifying p25 and the other encoding p27. After a considerable number of passages in culture, H9 cells chronically infected with the HTLV-IIIB isolate produced high levels of p25 and lower levels of p27. Passages in culture appear to select for a subpopulation of virus variants that specify high levels of p25 Nef expression.
Zweig
M
M
Nucleic Acid and Protein Synthesis Laboratory, Program Resources, Inc., NCI-Frederick Cancer Research and Development Center, Maryland 21702.
Samuel
K P
KP
Showalter
S D
SD
Bladen
S V
SV
DuBois
G C
GC
Lautenberger
J A
JA
Hodge
D R
DR
Papas
T S
TS
D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D015693:Gene Products, nef / Q000096:biosynthesis / Q000302:isolation & purification*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008970:Molecular Weight; D054311:nef Gene Products, Human Immunodeficiency Virus
D015693:Gene Products, nef; D054311:nef Gene Products, Human Immunodeficiency Virus