Glutathione transferase isoenzymes from human prostate.
By using affinity-chromatography and isoelectric-focusing techniques, several forms of glutathione transferase (GSTs) were resolved from human prostate cytosol. All the three major classes of GST, i.e. Alpha, Mu and Pi, are present in human prostate. However, large inter-individual variation in the qualitative and quantitative expression of different isoenzymes resulted in the samples investigated. The most abundant group of prostate isoenzymes showed acid (pI 4.3-4.7) behaviour and were classified as Pi class GSTs on the basis of their immunological and structural properties. Immunohistochemical staining of Pi class GSTs was prevalently distributed in the epithelial cells surrounding the alveolar lumen. Class Mu GSTs are also expressed, although in small amounts and in a limited number of samples, by human prostate. The major cationic isoenzyme purified from prostate, GST-9.6; (pI 9.6; apparent subunit molecular mass of 28 kDa), appears to be different from the cationic GST alpha-epsilon forms isolated from human liver and kidney as evidenced by its structural, kinetical and immunological properties. This enzyme, which accounts for about 20-30% (on protein basis) of total amount of GSTs, is expressed by only 40% of samples. GST-9.6 has the ability to cross-react in immunoblotting analysis with antisera raised against rat liver GST 2-2, rather than with antisera raised against members of human Alpha, Mu and Pi class GSTs. Although prostate GST-9.6 shows close relationship with the human skin GST pI 9.9, it does not correspond to any other known human GST.
Di Ilio
C
C
Istituto di Scienze Biochimiche, Facoltà di Medicina, Università, G.D.'Annunzio, Chieti, Italy.
Aceto
A
A
Bucciarelli
T
T
Angelucci
S
S
Felaco
M
M
Grilli
A
A
Federici
G
G
D000368:Aged; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005982:Glutathione Transferase / Q000032:analysis* / Q000378:metabolism; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011467:Prostate / Q000201:enzymology*; D013379:Substrate Specificity
D007527:Isoenzymes; D005982:Glutathione Transferase