title: Primary structure of NADP-dependent malic enzyme in the dicotyledonous C4 plant Flaveria trinervia.
abstract: The primary structure of NADP-dependent malic enzyme (NADP-ME) of the dicotyledonous C4 plant Flaveria trinervia was determined from sequence analysis of a cDNA clone containing the complete coding region. Comparison of the mature F. trinervia NADP-ME with the maize enzyme reveals extensive sequence similarity. In contrast, no significant similarity can be detected between the putative transit peptides of the two enzymes. This suggests that the corresponding parts of the genes arose independently from each other during evolution of mono- and dicotyledonous C4 plants.
authors.affiliation: Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, Düsseldorf, FRG.
authors.forename: D
authors.initials: D
authors.lastname: Börsch
authors.forename: P
authors.initials: P
authors.lastname: Westhoff
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015723:Gene Library; D008291:Malate Dehydrogenase / Q000235:genetics*; D008969:Molecular Sequence Data; D009249:NADP / Q000378:metabolism; D010944:Plants / Q000201:enzymology* / Q000235:genetics; D011487:Protein Conformation; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D009249:NADP; D004247:DNA; D008291:Malate Dehydrogenase