Generation of small mutation in large genomic fragments by homologous recombination: description of the technique and examples of its use.
We have developed a technique of homologous recombination in bacteria which allows the mutagenesis of large genomic fragments cloned in cosmids. The desired mutation is first introduced into a plasmid clone and is then transferred to the appropriate cosmid clone by the means of double antibiotic selection coupled with phenotypic selection. We describe three different types of construct made by this technique.
Tripodi
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M
Dipartimento di Biopatholgia umana, Università la Sapienza, Rome, Italy.
Perfumo
S
S
Ali
R
R
Amicone
L
L
Abbott
C
C
Cortese
R
R
D003001:Cloning, Molecular; D003360:Cosmids; D004926:Escherichia coli / Q000235:genetics*; D005091:Exons; D015870:Gene Expression; D005821:Genetic Techniques; D016296:Mutagenesis; D010957:Plasmids; D011995:Recombination, Genetic