title: Determination of plasma alpha-amylase in the dog: a test of the specificity of new methods.
abstract: Commercially available chromogenic substrates for the assay of alpha-amylase were tested for specificity in dog plasma. Blocked alpha-4-nitrophenylmaltoheptaoside and non-blocked beta-4-nitrophenylmaltoheptaoside showed no interference with glucoamylase and were suitable for the measurement of alpha-amylase in dog plasma. In contrast, an alpha-4-nitrophenylmaltoheptaoside showed interference, and was therefore an inappropriate substrate. Reference values with the blocked substrate in a group of 82 non-selected 3 month- to 10 year-old male and female dogs were 355 +/- 131 U/l (mean +/- standard deviation) at 30 degrees C.
authors.affiliation: Laboratoire Associé INRA Toxicologie Biochimique et Métabolique, Ecole Nationale Vétérinaire, Toulouse, France.
authors.forename: J P
authors.initials: JP
authors.lastname: Braun
authors.forename: G
authors.initials: G
authors.lastname: Ouedraogo
authors.forename: B
authors.initials: B
authors.lastname: Thorel
authors.forename: C
authors.initials: C
authors.lastname: Médaille
authors.forename: A G
authors.initials: AG
authors.lastname: Rico
mesh_terms: D000818:Animals; D002850:Chromatography, Gel / Q000379:methods; D004285:Dogs; D005260:Female; D008297:Male; D012680:Sensitivity and Specificity; D013379:Substrate Specificity; D000516:alpha-Amylases / Q000097:blood*
chemical_list: D000516:alpha-Amylases