title: Binding of flunitrazepam to differentiating neurons cultured in a chemically defined, hormone-supplemented medium.
abstract: [3H]Flunitrazepam (FNZ) binding to cortical neurons from fetal rat brain was investigated in vitro. The use of a synthetic medium specific for neurons made it possible to plot a developmental curve of 3H-FNZ binding in an almost pure neuronal culture. Detectable specific binding was present in vitro at time 0 (that is, the 16th gestational day). A progressive increase of binding, due to an increment in the number of recognition sites, was observed on the subsequent days. The affinity of the specific binding sites to 3H-FNZ was enhanced by the addition of exogenous GABA, whereas the density was not affected.
authors.affiliation: Istituto di Neuropsichiatria, Università degli Studi di Palermo, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Savettieri
authors.forename: R
authors.initials: R
authors.lastname: Guarneri
authors.forename: G
authors.initials: G
authors.lastname: Salemi
authors.forename: V
authors.initials: V
authors.lastname: La Bella
authors.forename: D
authors.initials: D
authors.lastname: Ferraro
authors.forename: S
authors.initials: S
authors.lastname: Scondotto
authors.forename: F
authors.initials: F
authors.lastname: Piccoli
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D003470:Culture Media; D005445:Flunitrazepam / Q000378:metabolism*; D006728:Hormones / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
chemical_list: D003470:Culture Media; D006728:Hormones; D005680:gamma-Aminobutyric Acid; D005445:Flunitrazepam