Cell membrane DNA: a new target for psoralen photoadduct formation.
The effects of 8-methoxypsoralen plus long wavelength ultraviolet radiation on cell membrane DNA were examined. Treatment of human lymphocytes with 100 ng/ml 8-methoxypsoralen and 5 J/cm2 UVA led to the formation of 7.1 +/- 3.8 photoadducts per million bases. A monoclonal antibody, specific for 8-methoxypsoralen 4',5'-monoadducts, was used to detect photoadducts in the cell membrane DNA of human lymphocytes and three lymphoblastoid cell lines. Treatment of lymphocytes with 8-MOP and UVA reduced the lymphocyte DNA binding capacity by 56%. Cell membranes of normal lymphocytes were shown to contain three high affinity DNA binding proteins of 28, 59, and 79 kDa, respectively.
Gasparro
F P
FP
Department of Dermatology, Yale University, New Haven, CT 06510.
Dall'Amico
R
R
O'Malley
M
M
Heald
P W
PW
Edelson
R L
RL
D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism / Q000528:radiation effects; D004247:DNA / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D004268:DNA-Binding Proteins / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D008730:Methoxsalen / Q000494:pharmacology*; D014466:Ultraviolet Rays
D004268:DNA-Binding Proteins; D004247:DNA; D008730:Methoxsalen