title: Human follicular fluids contain tissue macrophages.
abstract: Tissue cells obtained from the follicular fluids of women undergoing in vitro fertilization and embryo transfer were found to contain interleukin-1 beta mRNA by Northern analysis. Since macrophages are known to produce interleukin-1 beta, we examined the follicular fluids of 20 women undergoing in vitro fertilization as well as tissue sections of normal human ovary for the presence of macrophages and monocytes. Although granulosa-luteal cells predominate in follicular fluid, we found that resident macrophages and monocytes comprise 5-15% of human follicular tissue cells. In addition, we observed that macrophages are present in the human ovarian follicle as well as in the corpus luteum.
authors.affiliation: Department of Obstetrics and Gynecology, University of Maryland, Baltimore 21201.
authors.forename: J A
authors.initials: JA
authors.lastname: Loukides
authors.forename: R A
authors.initials: RA
authors.lastname: Loy
authors.forename: R
authors.initials: R
authors.lastname: Edwards
authors.forename: J
authors.initials: J
authors.lastname: Honig
authors.forename: I
authors.initials: I
authors.lastname: Visintin
authors.forename: M L
authors.initials: ML
authors.lastname: Polan
mesh_terms: D015152:Blotting, Northern; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D005434:Flow Cytometry; D015571:Follicular Fluid / Q000166:cytology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007375:Interleukin-1 / Q000032:analysis* / Q000235:genetics; D008183:Luteal Phase; D008264:Macrophages / Q000737:chemistry*; D008597:Menstrual Cycle; D009000:Monocytes / Q000737:chemistry*; D010053:Ovary / Q000166:cytology; D012333:RNA, Messenger / Q000032:analysis*
chemical_list: D007375:Interleukin-1; D012333:RNA, Messenger