[The differentiation of the nerve and glial cells of neocortical transplants placed into the brain of adult rats].
Embryonal neural tissue of 17-day-old rat embryos was transplanted into the brain of adult Wistar rats to test the differentiation of transplants with reference to the normal cerebral cortex development. The control and the experimental rats were decapitated 2, 5, 7, 10, 15, 20, 25, and 35 days after the transplantation. Differentiation of neural tissue was studied using monoclonal antibodies against neurofilaments as well as by counting the proportion of differentiated neurons. The glial differentiation was studied by immunohistochemical method using monoclonal antibodies against acid glial fibrillar protein and vimentin. The differentiation of neural cells of transplants proved to be synchronous with the normal ones while the differentiation of glial cells accelerates.
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D000818:Animals; D000911:Antibodies, Monoclonal; D002454:Cell Differentiation; D002540:Cerebral Cortex / Q000166:cytology / Q000276:immunology / Q000637:transplantation*; D004622:Embryo, Mammalian; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D009457:Neuroglia / Q000166:cytology* / Q000276:immunology; D009474:Neurons / Q000166:cytology* / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D000911:Antibodies, Monoclonal
Differentsirovka nervnykh i glial'nykh kletok neokortikal'nykh transplantatov, pomeshchennykh v mozg vzroslykh krys.