title: The use of a multichannel scaling method to detect radioactive species in spread monolayers.
abstract: This study describes a simple and inexpensive method for monitoring radioactive species spread as monolayers at the air/water interface. The combination of a discriminator and multichannel scalar counter with a personal computer functions to unify all measurements, to simplify the operational process and data acquisition, and to provide a real-time display of the data. Its use is demonstrated by following the hydrolysis of L-alpha-[1-14C]dioleoyl phosphatidylcholine by the enzyme, phospholipase A2, isolated from the porcine pancreas.
authors.affiliation: Department of Pediatrics, University of Wisconsin, Madison 53706.
authors.forename: F H
authors.initials: FH
authors.lastname: Tsao
authors.forename: T K
authors.initials: TK
authors.lastname: Foo
authors.forename: P M
authors.initials: PM
authors.lastname: DeLuca
authors.forename: G
authors.initials: G
authors.lastname: Zografi
mesh_terms: D000818:Animals; D002250:Carbon Radioisotopes / Q000032:analysis; D006868:Hydrolysis; D066298:In Vitro Techniques; D008567:Membranes, Artificial; D010179:Pancreas / Q000201:enzymology; D010713:Phosphatidylcholines / Q000032:analysis; D010741:Phospholipases A / Q000032:analysis; D054467:Phospholipases A2; D011868:Radioisotopes / Q000032:analysis*; D013552:Swine
chemical_list: D002250:Carbon Radioisotopes; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D011868:Radioisotopes; D010741:Phospholipases A; D054467:Phospholipases A2; C017251:1,2-oleoylphosphatidylcholine