Anti-oxidative profile of lobenzarit disodium (CCA).
The effects of lobenzarit disodium (CCA) on various species of activated oxygen were investigated in chemiluminescence experiments. CCA showed a quenching effect against hydroxyl radicals generated by Fenton reaction. The inhibition of CCA was much more intense than that of mefenamic acid which is an anti-inflammatory drug and an analogous compound to CCA. CCA also showed a quenching effect against singlet oxygen generated in enzymatic systems. However, CCA had no effect against superoxide anion radicals generated in the xanthine oxidase-hypoxanthine system. As a model of lipid peroxidation and protein alteration induced by activated oxygen, we examined the auto-oxidation of linolenic acid and the UV irradiation of immunoglobulin G (IgG). CCA inhibited the production of lipid peroxide; however CCA did not show a direct quenching action against lipid radicals which had been previously generated. CCA also inhibited the IgG alteration induced by UV irradiation. These results indicate that CCA has anti-oxidative actions with specificity for activated oxygen species and that CCA protects against lipid and protein damage induced by activated oxygen.
Cynshi
O
O
Fujigotemba Research Laboratories, Chugai Pharmaceutical Co., Ltd, Shizuoka, Japan.
Saitoh
M
M
Cynshi
F
F
Tanemura
M
M
Hata
S
S
Nakano
M
M
D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D005439:Flufenamic Acid / Q000494:pharmacology; D007074:Immunoglobulin G / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008163:Luminescent Measurements; D008528:Mefenamic Acid / Q000494:pharmacology; D062367:ortho-Aminobenzoates / Q000737:chemistry / Q000494:pharmacology*
D000975:Antioxidants; D007074:Immunoglobulin G; D062367:ortho-Aminobenzoates; D008528:Mefenamic Acid; D005439:Flufenamic Acid; C015735:lobenzarit