The interpretation of site-directed mutagenesis experiments by linear free energy relations.
Fersht and co-workers have applied a linear free energy relation (Brønsted equation) to analyze site-directed mutagenesis experiments involving the enzyme tyrosyl-tRNA synthetase and have suggested that the Brønsted exponent is linearly correlated with the value of the reaction coordinate at the transition state. We point out that when the mutants differ solely through the formation or deletion of a hydrogen bond away from the reaction center, a linear free energy relation is expected only in limiting cases for which the Brønsted relation exponent is 0, 1 or infinity. The results may be correlated with a conformational coordinate but not with the development of the reaction coordinate per se.
Straub
J E
JE
Department of Chemistry, Harvard University, Cambridge, MA 02138.
Karplus
M
M
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D006860:Hydrogen Bonding; D016297:Mutagenesis, Site-Directed; D015202:Protein Engineering; D014448:Tyrosine-tRNA Ligase / Q000737:chemistry / Q000235:genetics*
D014448:Tyrosine-tRNA Ligase