Investigation of dye/protein interaction and its application to enzyme purification.
In this review the results of the interaction of the active dyes used in the USSR textile industry with microbial enzymes and blood serum proteins are discussed. The complexity of dye/protein interaction and the dependence of this interaction on different factors is demonstrated. Some practical aspects of the use of dye containing sorbents are presented and discussed. Their suitability for RNA ligase and DNA ligase, acetate kinase, alcohol dehydrogenase, lactate dehydrogenase and glucose-6-phosphate dehydrogenase purification and blood serum protein fractionation is demonstrated.
Glemza
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AA
Department of Plant Physiology and Microbiology, Vilnius University, Lithuania, USSR.
D000327:Adsorption; D002846:Chromatography, Affinity; D002942:Circular Dichroism; D004396:Coloring Agents; D004798:Enzymes / Q000302:isolation & purification*; D011506:Proteins
D004396:Coloring Agents; D004798:Enzymes; D011506:Proteins