title: The complete DNA sequence of vaccinia virus.
abstract: The complete DNA sequence of the genome of vaccinia virus has been determined. The genome consisted of 191,636 bp with a base composition of 66.6% A + T. We have identified 198 "major" protein-coding regions and 65 overlapping "minor" regions, for a total of 263 potential genes. Genes encoded by the virus were located by examination of DNA sequence characteristics and compared with existing vaccinia virus mapping analyses, sequence data, and transcription data. These genes were found to be compactly organized along the genome with relatively few regions of noncoding sequences. Whereas several similarities to proteins of known function were discerned, the function of the majority of proteins encoded by these open reading frames is as yet undetermined.
authors.affiliation: Virogenetics Corporation, Troy, New York 12180-8349.
authors.forename: S J
authors.initials: SJ
authors.lastname: Goebel
authors.forename: G P
authors.initials: GP
authors.lastname: Johnson
authors.forename: M E
authors.initials: ME
authors.lastname: Perkus
authors.forename: S W
authors.initials: SW
authors.lastname: Davis
authors.forename: J P
authors.initials: JP
authors.lastname: Winslow
authors.forename: E
authors.initials: E
authors.lastname: Paoletti
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D014176:Protein Biosynthesis; D015183:Restriction Mapping; D014616:Vaccinia virus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins