Comparison of islet cell antibodies, islet cell surface antibodies and anti-bovine serum albumin antibodies in type 1 diabetes.
The present study was undertaken to compare the changes in islet cell antibodies (ICA), islet cell surface antibodies using rat insulinoma cells (RINr-ICSA), and anti-bovine serum albumin antibodies (BSA-Ab) in the clinical course of type 1 (insulin-dependent) diabetes. Sera were obtained from 57 patients with type 1 diabetes and 47 normal controls. ICA, RINr-ICSA and BSA-Ab were detected by an enzymatic immunohistochemical method, an indirect immunofluorescence method and an enzyme-linked immunosorbent assay, respectively. The incidence of ICA significantly decreased with the duration of diabetes: less than 1 year: 5/6 (83%); 1-2 years: 3/6 (50%); 2-3 years; 2/6 (33%); greater than 3 years 6/39 (15%). There was a significant positive correlation between RINr-ICSA and BSA-Ab (P less than 0.05). These findings suggest that RINr-ICSA or BSA-Ab may be produced by some similar immune mechanism which is, however, different from ICA, and that they have no direct relation to the clinical course of diabetes.
Yokota
A
A
First Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
Yamaguchi
Y
Y
Ueda
Y
Y
Nakanishi
T
T
Ikari
N
N
Akazawa
S
S
Miyake
S
S
Nagataki
S
S
D000293:Adolescent; D000328:Adult; D000906:Antibodies / Q000032:analysis* / Q000276:immunology; D001323:Autoantibodies / Q000032:analysis*; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D005260:Female; D006801:Humans; D007340:Insulinoma / Q000276:immunology / Q000473:pathology; D008297:Male; D012710:Serum Albumin, Bovine / Q000276:immunology*
D000906:Antibodies; D001323:Autoantibodies; C024211:islet cell antibody; D012710:Serum Albumin, Bovine