MK-801-induced sprouting by CGRP immunoreactive primary afferent fibers in the dorsal spinal cord of the rat.
In the present study, rats received daily injections of the N-methyl-D-aspartate (NMDA) receptor antagonist, MK-801, over 30 consecutive days. The effects of MK-801 on the distribution of calcitonin gene-related peptide (CGRP)-immunoreactive fibers in the dorsal spinal cord of the rat were subsequently examined. In addition to the normal immunostaining pattern in laminae I, II and lateral V, a dense network of CGRP-immunoreactive fibers was observed along the medial border of the dorsal horn and within the dorsal grey commissure. This marked increase in immunoreactivity was virtually eliminated following dorsal rhizotomy. These observations suggest that MK-801 induces intraspinal sprouting by CGRP immunoreactive primary afferent fibers in vivo.
Harris
C H
CH
Department of Anatomical Sciences, University of Oklahoma, Oklahoma City 73190.
Fagan
E L
EL
Shew
R L
RL
Kammerlocher
T C
TC
McNeill
D L
DL
D000344:Afferent Pathways / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis*; D003292:Convulsants / Q000494:pharmacology; D016291:Dizocilpine Maleate / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D008297:Male; D009412:Nerve Fibers / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects* / Q000502:physiology
D003292:Convulsants; D016291:Dizocilpine Maleate; D015740:Calcitonin Gene-Related Peptide