Action of catalase on peroxyacetic acid. Kinetic studies.
The activity of catalase on peroxoacetic acid was studied by a kinetic point of view. The "inactivation-reactivation" sequence consists in two first order (intramolecular) one-electron redox reactions. In the catalytic cycle, the reaction producing Compound I is, as espected, a second order process rate determined by the enzyme-peroxide adduct formation step, whereas, surprisingly, the reaction restoring the enzyme resting state is a first order process in which the limiting step is the 0-0 linkage breaking inside the Compound I-peroxide adduct.
Ferri
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Istituto di Chimica Biologica, Universita Degli Studi Via L. Borsari, Ferrara, Italy.
D000818:Animals; D002374:Catalase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D004793:Enzyme Reactivators; D066298:In Vitro Techniques; D007700:Kinetics; D010084:Oxidation-Reduction; D010463:Peracetic Acid / Q000737:chemistry; D013053:Spectrophotometry; D013379:Substrate Specificity
D004793:Enzyme Reactivators; D002374:Catalase; D010463:Peracetic Acid