title: Histamine H1-receptor-like binding sites in the locust nervous tissue.
abstract: The histamine H1-receptor-like binding sites in the nervous tissue of the locust Locusta migratoria were investigated with a conventional radio-receptor assay using [3H]mianserin as the radio ligand. Binding of [3H]mianserin to the binding site is sensitive to proteases and heat treatment. It shows the characteristics of ligand-receptor interactions. The single binding site has high affinity for mianserin (KD = 7.05 nM) and is present in high concentrations (Bmax = 1.53 pmol/mg) in the whole nervous tissue. All tested antihistamines have a high affinity for the binding site, which suggests that it represents an insect histamine receptor. Nevertheless, it shows its peculiarities distinguishing it from vertebrate histamine H1-receptors.
authors.affiliation: Universität Hamburg, Zoologisches Institut, F.R.G.
authors.forename: T
authors.initials: T
authors.lastname: Roeder
mesh_terms: D000818:Animals; D001665:Binding Sites; D006110:Grasshoppers / Q000378:metabolism*; D006633:Histamine Antagonists / Q000378:metabolism; D008803:Mianserin / Q000037:antagonists & inhibitors / Q000378:metabolism; D009417:Nerve Tissue / Q000378:metabolism*; D011869:Radioligand Assay; D011969:Receptors, Histamine H1 / Q000378:metabolism*
chemical_list: D006633:Histamine Antagonists; D011969:Receptors, Histamine H1; D008803:Mianserin