Analysis of 8-methoxypsoralen by high-performance liquid chromatography.
We report a simple and rapid procedure for assaying 8-methoxypsoralen (8-MOP) in plasma by high-performance liquid chromatography (HPLC). The standard curve for the assay is linear for 8-MOP from 15 to 500 micrograms/L (y = 0.002x-0.01, r = 0.99) with a lower limit of detection of 1.5 micrograms/L. Intra-assay precision (CV) was 6.0% at the 100 micrograms/L concentration and 10.0% at 50 micrograms/L (n = 30 each). Interassay precision was 6.4% at 100 micrograms/L and 7.0% at 50 micrograms/L (n = 50 each). Extraction recovery of 8-MOP was 98%. Common antiarrhythmics, sedatives, and hypnotics were found not to interfere.
Ketchum
C H
CH
Department of Pathology, University of Alabama, Birmingham 35294.
Robinson
C A
CA
Huang
S T
ST
D000078223:5-Methoxypsoralen; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D008730:Methoxsalen / Q000302:isolation & purification* / Q000378:metabolism
D000078223:5-Methoxypsoralen; D008730:Methoxsalen