Systematic classification of HIV biological subtypes on lymphocytes and monocytes/macrophages.
The growth properties and cytopathic effects of several HIV-1 and HIV-2 strains were compared between cultures on human lymphocytes and monocytes/macrophages, respectively. For some isolates (among these three paired isolates from blood and cerebrospinal fluid) replication and cytopathogenicity were comparable between lymphocytes and monocytes/macrophages (dual tropic viruses), while others showed a very specific tropism for only one cell type. Yet another subtype grew neither well on lymphocytes nor on macrophages. Taking into account the growth properties in monocytes/macrophages we propose a classification system for HIV subtypes on these cells (alpha-delta), in analogy to the nomenclature for HIV-subtyping on lymphocytes (a-d). Using this system, some prototypic viruses (LAV/HTLV-IIIB, HIV-2ROD, SIVBK28, HIV-2ALT) as well as several other HIV-1 and HIV-2 isolates were subtyped.
von Briesen
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H
Chemotherapeutisches Forschungsinstitut Georg-Speyer-Haus, Frankfurt, Federal Republic of Germany.
Andreesen
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Rübsamen-Waigmann
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H
D002478:Cells, Cultured; D002648:Child; D005260:Female; D015497:HIV-1 / Q000145:classification* / Q000254:growth & development / Q000648:ultrastructure; D015498:HIV-2 / Q000145:classification* / Q000254:growth & development / Q000648:ultrastructure; D006801:Humans; D008264:Macrophages / Q000382:microbiology* / Q000648:ultrastructure; D009000:Monocytes / Q000382:microbiology* / Q000648:ultrastructure; D012703:Serotyping; D009626:Terminology as Topic; D014779:Virus Replication