[A new method of purification of L-lysine-alpha-oxidase from the fungus Trichoderma].
A new procedure is developed for purification of the antitumoral enzyme L-lysyl-alpha-oxidase from Trichoderma sp. The procedure included two steps: hydrophobic chromatography on butyl sylochrome C-80 and chromatography using biospecific sorbent AH-Sepharose. The simplified procedure enabled to increase distinctly the specific enzymatic activity from 30 to 50 IU/mg in the preparation obtained with a good yield.
Laugalene
N F
NF
Vesa
V S
VS
Iankiavichene
R P
RP
Puodzhiute
S P
SP
Sudzhiuvene
O F
OF
Pesliakas
I I
II
Khaduev
S Kh
SKh
Berezov
T T
TT
D000594:Amino Acid Oxidoreductases / Q000302:isolation & purification*; D002845:Chromatography / Q000379:methods; D014242:Trichoderma / Q000201:enzymology*
D000594:Amino Acid Oxidoreductases; C041123:L-lysine oxidase
Novyĭ metod ochistki L-lizin-alpha-oksidazy griba Trichoderma.