Quantitation of human immunodeficiency virus DNA by using the polymerase chain reaction.
The polymerase chain reaction was used to measure the DNA copy number of human immunodeficiency virus (HIV). Differences in polymerase chain reaction amplification efficiency were controlled by amplifying known amounts of HIV DNA in parallel with samples. This technique is a sensitive, accurate, and reproducible method for the quantitation of HIV DNA.
Dickover
R E
RE
Department of Internal Medicine, University of California, Davis 95616.
Donovan
R M
RM
Goldstein
E
E
Dandekar
S
S
Bush
C E
CE
Carlson
J R
JR
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics / Q000592:standards; D005069:Evaluation Studies as Topic; D005784:Gene Amplification; D006678:HIV / Q000032:analysis* / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012015:Reference Standards
D004279:DNA, Viral