Isolation and characterization of the recA gene of Bordetella pertussis.
This report describes the detection and cloning of the Bordetella pertussis recA gene. Escherichia coli clones having recombinant plasmids containing the B. pertussis recA gene were isolated by complementing an E. coli RecA- mutant's inability to survive in the presence of methylmethanesulphonate (MMS). This gene was shown to complement the deficiency of E. coli RecA- strains to tolerate the DNA-damaging effects of both a chemical agent and ultraviolet light (u.v.). Deletion mapping experiments localized the gene to a 2.5 kb StuI-EcoRI fragment, and expression of the gene in E. coli resulted in the production of a 40 kD protein. These data strongly suggest that a region of the B. pertussis chromosome that encodes RecA-like activity has been isolated and cloned.
Kuhl
S A
SA
Department of Microbiology and Immunology, College of Medicine, University of Arizona, Tucson 85724.
McCreary
R P
RP
Bannan
J D
JD
Friedman
R L
RL
D015139:Blotting, Southern; D001886:Bordetella pertussis / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular; D004249:DNA Damage; D004269:DNA, Bacterial; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004352:Drug Resistance, Microbial; D005798:Genes, Bacterial / Q000528:radiation effects; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D009154:Mutation; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011938:Rec A Recombinases / Q000235:genetics*; D015183:Restriction Mapping; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D008741:Methyl Methanesulfonate; D011938:Rec A Recombinases; D004259:DNA-Directed DNA Polymerase