[Construction of recombinant genes coding for products containing the fragment of Staphylococcus aureus protein A and the fragment of Pseudomonas aeruginosa exotoxin A].
The genes coding for Pseudomonas aeruginosa exotoxin-A and Staphylococcus aureus A-protein have been cloned. Different fragments of the genes were subcloned on the plasmid vestors. The plasmids pAPA4 and pAPA42 have been constructed. The plasmids are coding for the hybrid protein which consists of the immunoglobulin binding A-protein domain at its NH2-terminus and the catalytically active fragment of exotoxin-A at its COON-terminus. The hybrid gene is expressed in Escherichia coli cells under the control of lambda cro-gene expression elements (pAPA42) or lac-operon expression elements (pAPA4). The latter proved to be most productive.
Zdanovskiĭ
A G
AG
Zdanovskaia
M V
MV
Iankovskiĭ
N K
NK
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D005098:Exotoxins / Q000096:biosynthesis* / Q000235:genetics; D015151:Immunoblotting; D010957:Plasmids; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D013205:Staphylococcal Protein A / Q000096:biosynthesis* / Q000235:genetics; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D005098:Exotoxins; D011994:Recombinant Proteins; D013205:Staphylococcal Protein A; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
Konstruirovanie rekombinantnykh genov, produkty kotorykh soderzhat v svoem sostave fragment A belka Staphylococcus aureus i fragment ékzotoksina A Pseudomonas aeruginosa.