A new fluorescence enhancement solution for europium-based time-resolved fluoroimmunoassays.
A cationic detergent is proposed as suitable insulating agent for the preparation of a fluorescence enhancement solution useful for europium-based time-resolved fluoroimmunoassays. Luminescence from europium ions at concentration as low as 0.5 pmol/l can be detected in solution containing 1.6 mmol/l thenoyltrifluoroacetone, 110.5 mumol/l Adogen 464 and 0.1% Tween 20, in the presence of 0.5 mol/l NaCl. A competitive TR-FIA for rabbit IgG is described, showing that the new enhancement solution allows a sensitivity comparable to that of the high performance LKB Wallac DELFIA.
Degan
P
P
IST, Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
Abbondandolo
A
A
Montagnoli
G
G
D000818:Animals; D002614:Chelating Agents; D003902:Detergents; D005063:Europium; D005455:Fluorescent Antibody Technique; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000032:analysis; D008163:Luminescent Measurements; D008823:Micelles; D009994:Osmolar Concentration; D011817:Rabbits; D012965:Sodium Chloride
D002614:Chelating Agents; D003902:Detergents; D007074:Immunoglobulin G; D008823:Micelles; D005063:Europium; D012965:Sodium Chloride