Detection of lipopolysaccharides blotted to polyvinylidene difluoride membranes.
Bacterial lipopolysaccharide (LPS) blotted to polyvinylidene difluoride (PVDF) membranes was detected by a technique adapted from current methodologies used to detect glycoproteins. PVDF-bound LPS was coupled to a hapten and localized on the membrane by Western blotting with an antibody-alkaline phosphatase conjugate specific for the hapten. Immobilon blots could be made reversibly transparent for photography and densitometry.
Stein
M A
MA
Department of Microbiology, Immunology and Parasitology, Louisiana State University Medical Center, New Orleans 70112-1393.
McAllister
S A
SA
Johnston
K H
KH
Diedrich
D L
DL
D000469:Alkaline Phosphatase / Q000378:metabolism; D015153:Blotting, Western / Q000379:methods*; D006241:Haptens; D008070:Lipopolysaccharides / Q000032:analysis*; D008567:Membranes, Artificial; D011145:Polyvinyls; D005465:Fluorocarbon Polymers
D000469:Alkaline Phosphatase; D006241:Haptens; D008070:Lipopolysaccharides; D008567:Membranes, Artificial; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers