Comparison of alkaline phosphatase-conjugated oligonucleotide DNA probe with the Sereny test for identification of Shigella strains.
We compared an alkaline phosphatase-conjugated oligonucleotide DNA probe with the Sereny test to determine the sensitivity and specificity of the probe in detecting virulent Shigella strains. The probe hybridized with all 52 Sereny-test-positive strains (sensitivity, 100%) and 4 of 21 Sereny-test-negative strains (specificity, 81%). The probe did not hybridize with any of the Sereny-test-negative S. dysenteriae type 1 strains. This nonradioactive, synthetic probe provides a simple, rapid way to test a large number of strains simultaneously in a field setting, which will contribute to an improved understanding of the epidemiologic patterns of shigellosis in developing countries.
Panda
C S
CS
Department of Microbiology, Lady Hardinge Medical College, New Delhi, India.
Riley
L W
LW
Kumari
S N
SN
Khanna
K K
KK
Prakash
K
K
D000469:Alkaline Phosphatase; D000818:Animals; D001431:Bacteriological Techniques; D001483:Base Sequence; D015342:DNA Probes; D004405:Dysentery, Bacillary / Q000175:diagnosis; D005069:Evaluation Studies as Topic; D006168:Guinea Pigs; D006801:Humans; D007637:Keratoconjunctivitis / Q000209:etiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides; D012760:Shigella / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D013045:Species Specificity; D014774:Virulence / Q000235:genetics
D015342:DNA Probes; D009838:Oligodeoxyribonucleotides; D000469:Alkaline Phosphatase