title: An enzyme-linked immunosorbent assay for the measurement of human interleukin-6.
abstract: An enzyme-linked immunosorbent assay has been developed to measure human interleukin-6. The assay, based on the avidin-biotin amplified two-step sandwich method, is quick (requiring 4.5 h), sensitive (detecting 9.5 pg/ml) and satisfactory in reproducibility and specificity. It shows good correspondence with the results of bioassays, and it is not affected by serum and plasma components. These results indicate that this ELISA is suitable for application to clinical samples, which is a major advantage over the widely used bioassays.
authors.affiliation: Basic Research Laboratories, Toray Industries, Inc., Kanagawa, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Ida
authors.forename: S
authors.initials: S
authors.lastname: Sakurai
authors.forename: T
authors.initials: T
authors.lastname: Hosaka
authors.forename: K
authors.initials: K
authors.lastname: Hosoi
authors.forename: T
authors.initials: T
authors.lastname: Kunitomo
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuura
authors.forename: M
authors.initials: M
authors.lastname: Kohase
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001681:Biological Assay; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D015850:Interleukin-6 / Q000032:analysis* / Q000097:blood; D051379:Mice; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature
chemical_list: D000911:Antibodies, Monoclonal; D015850:Interleukin-6