Effects of trifluoroacetic acid, a halothane metabolite, on C6 glioma cells.
Effects of trifluoroacetic acid (TFA) on cell growth, DNA, glycoprotein, and dolichol-linked oligosaccharides synthesis and ribonucleotide triphosphate concentrations were examined in exponentially growing C6 murine glioma cells. One day of treatment with TFA caused a slight concentration-dependent enhancement of cell growth and [3H]thymidine incorporation. Exposure for 1 or 5 d to TFA (0.5-7.0 mM) elevated the [3H]leucine incorporation in a dose- and time-dependent manner. The results suggested that TFA stimulated cell growth and enhanced protein synthesis. TFA also affected [3H]mannose incorporation into glycoproteins and dolichol-linked oligosaccharides in a dose-dependent fashion. In addition, it was found that TFA accelerated lectin-induced cell agglutination. These data suggest that TFA, the principle halothane metabolite, alters plasmalemmal glycoprotein synthesis. These findings should form a basis for further understanding on the mechanism underlying halothane-associated neurotoxicity.
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Department of Anatomical Sciences and Neurobiology, University of Louisville, KY 40292.
Ling
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McClure
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GD
Tseng
M T
MT
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004273:DNA, Neoplasm / Q000187:drug effects / Q000378:metabolism; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006023:Glycoproteins / Q000096:biosynthesis*; D006031:Glycosylation / Q000187:drug effects; D007930:Leucine / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D051379:Mice; D009844:Oligosaccharides / Q000096:biosynthesis*; D013936:Thymidine / Q000378:metabolism; D014269:Trifluoroacetic Acid / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D004273:DNA, Neoplasm; D006023:Glycoproteins; D009844:Oligosaccharides; D014269:Trifluoroacetic Acid; D007930:Leucine; D008358:Mannose; D013936:Thymidine