title: Structure of the type 5 capsular polysaccharide of Staphylococcus aureus.
abstract: The Staphylococcus aureus type 5 capsular polysaccharide is composed of 2-acetamido-2-deoxy-L-fucose (1 part), 2-acetamido-2-deoxy-D-fucose (1 part), and 2-acetamido-2-deoxy-D-mannuronic acid (1 part). On the basis of methylation analysis, optical rotation, high-field one- and two-dimensional 1H- and 13C-n.m.r. experiments, and selective cleavage with 70% aqueous hydrogen fluoride, the polysaccharide was found to be a partially O-acetylated (50%) polymer of the repeating trisaccharide unit, [----4)-3-O-Ac-beta-D-ManpNAcA-(1----4)-a-L-FucpNAc-(1----3) -beta-D-FucpNAc-(1----]n.
authors.affiliation: Office of Biologics Research and Review, Food and Drug Administration, Bethesda, Maryland 20892.
authors.forename: M
authors.initials: M
authors.lastname: Moreau
authors.forename: J C
authors.initials: JC
authors.lastname: Richards
authors.forename: J M
authors.initials: JM
authors.lastname: Fournier
authors.forename: R A
authors.initials: RA
authors.lastname: Byrd
authors.forename: W W
authors.initials: WW
authors.lastname: Karakawa
authors.forename: W F
authors.initials: WF
authors.lastname: Vann
mesh_terms: D002240:Carbohydrate Sequence; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D003641:Deamination; D004590:Electrophoresis, Paper; D006858:Hydrofluoric Acid; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009608:Nitrous Acid; D011135:Polysaccharides, Bacterial; D013211:Staphylococcus aureus
chemical_list: D011135:Polysaccharides, Bacterial; D006858:Hydrofluoric Acid; D009608:Nitrous Acid