Rat DNA polymerase beta gene can join in excision repair of Escherichia coli.
Though DNA polymerase I (poll) of Escherichia (E.) coli is understood to play a role in repair synthesis of excision repair, it is still obscure whether DNA polymerase beta (pol beta) plays a similar role in eukaryotic cells. To estimate the role of pol beta in excision repair processes, we inserted the rat pol beta gene into several mutant E. coli defective in a diverse set of enzymatic activities of poll. UV resistance was seen only when the 5'----3' exonuclease (exo) activity of poll molecules remained. Therefore it is suggested that 5'----3' exo activity as well as pol beta activity are essential for repair synthesis of excision repair in eukaryotic cells.
Ohnishi
T
T
Department of Biology, Nara Medical University, Japan.
Yuba
S
S
Date
T
T
Utsumi
H
H
Matsukage
A
A
D000818:Animals; D004249:DNA Damage; D004256:DNA Polymerase I / Q000235:genetics* / Q000378:metabolism; D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D009154:Mutation; D010957:Plasmids; D051381:Rats; D015183:Restriction Mapping; D014162:Transfection; D014169:Transformation, Bacterial; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D004256:DNA Polymerase I