DNA adduct formation in human and mouse skin by mixtures of polycyclic aromatic hydrocarbons.
32P-Postlabelling analysis has been used to detect the formation in vivo of DNA adducts by components of complex mixtures of polycyclic aromatic hydrocarbons (PAHs) in coal-tar, creosote, bitumen, juniper tar, used engine oils and fuel exhaust condensates. The presence of DNA adducts derived from these agents has been investigated in mouse skin, in human skin explants maintained in short-term organ culture and in human skin in vivo, and the formation of many different PAH-DNA adducts was observed. Similar levels and patterns of adducts were found in DNA from human skin to those in mouse skin, which is known to be susceptible to the carcinogenic activity of PAH mixtures, thus demonstrating the potential hazard to man of epidermal contact with these materials.
Phillips
D H
DH
Chester Beatty Laboratories, Institute of Cancer Research, London, UK.
Schoket
B
B
Hewer
A
A
Grover
P L
PL
D000818:Animals; D002273:Carcinogens; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism*; D006801:Humans; D051379:Mice; D011083:Polycyclic Compounds / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*
D002273:Carcinogens; D011083:Polycyclic Compounds; D004247:DNA