Production of plasmid DNA in a simple, inexpensive and reusable fermentor.
Performance data for the production of plasmid DNA in bacterial cultures have been obtained using a new concept of simplified fermentor, the Lofstrand Bactolift. The unique design of this fermentor incorporates a standard 500-ml or 1-liter centrifuge bottle as the bacterial growth vessel, thus eliminating the necessity to transfer the cultures for subsequent processing. Bacterial and plasmid yields have been shown to be equal to or greater than those obtained using conventional shake flasks. Twelve 1-liter Bactolift fermentors can be operated in a standard laboratory water bath that occupies less than two square feet of bench space, as compared to a limit of six 2.8-liter Fernbach flasks containing one liter of culture held in an incubator shaker cabinet that occupies nine square feet of laboratory floor space.
Alexander
R L
RL
Lofstrand Labs Limited, Gaithersburg, MD 20879.
Smith
R G
RG
D001431:Bacteriological Techniques / Q000295:instrumentation*; D001709:Biotechnology; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004926:Escherichia coli / Q000032:analysis / Q000235:genetics / Q000254:growth & development; D005069:Evaluation Studies as Topic; D005285:Fermentation; D010957:Plasmids
D004269:DNA, Bacterial