Extrachromosomal HIV-1 DNA in persistently infected U937 cells.
Persistent human immunodeficiency virus type 1 (HIV-1) infection of U937 monocytic cells resulted in the accumulation of novel forms of extrachromosomal viral DNA. These DNA species are larger than the genome size of HIV-1 and persist indefinitely. The extrachromosomal viral DNA species (E-DNA) were shown to be structurally stable by subcloning of infected cell lines and restriction fragment analysis. Similar E-DNA structures were observed in independent infections. Persistently infected monocytic cells had low levels of viral antigens, reflecting the low levels of viral RNA that were detected. These results support a role for E-DNA in persistent HIV-1 infection of monocytic cells.
Pauza
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CD
Developmental Biology Laboratory, Salk Institute for Biological Studies, San Diego, CA 92138-9216.
Singh
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MK
D002460:Cell Line; D004279:DNA, Viral / Q000378:metabolism*; D005111:Extrachromosomal Inheritance; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D009000:Monocytes / Q000382:microbiology*; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis*
D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral