Phagocytosis of opsonized fluorescent microspheres by equine polymorphonuclear leukocytes.
Equine blood polymorphonuclear leukocytes (PMN) were isolated by buffy coat and hypotonic lysis of residual erythrocytes. A highly reproducible method is described for measuring the uptake of opsonized latex microspheres by equine PMN using flowcytometry. The use of cytochalasin D allowed for differentiation of ingested from attached particles. The kinetics of phagocytosis in vitro is shown for different experimental conditions. We developed an assay for evaluation of phagocytic capacity of PMN which allows the assessment of drugs for their influence on phagocytosis in vivo as well as in vitro.
Foerster
R J
RJ
Institute for Medical Microbiology, Infectious and Epidemic Diseases, Veterinary Faculty, University of Munich, München, FRG.
Wolf
G
G
D000818:Animals; D002478:Cells, Cultured; D005434:Flow Cytometry; D006736:Horses / Q000097:blood / Q000276:immunology*; D008863:Microspheres; D009504:Neutrophils / Q000276:immunology*; D010587:Phagocytosis / Q000502:physiology*