N-glycosylated and unglycosylated forms of caprine trophoblast protein-1 are secreted by preimplantation goat conceptuses.
Goat conceptuses secrete caprine trophoblast protein-1 (cTP-1) which is related antigenically to the abundant embryonic interferon-alpha II of sheep and cattle. Antiserum to ovine and bovine TP-1s immunoprecipitated three molecular weight classes (23,000, 21,000 and 17,000, each with two isotypes) of cTP-1 from goat conceptus culture medium. Cultures which contained tunicamycin resulted in a shift in the Mr = 23,000 and Mr = 21,000 forms to a Mr of 17,000. The Mr = 23,000 and 21,000 forms, but not the Mr = 17,000 form, bound to Concanaval in A-Sepharose and were eluted under conditions selective for glycoproteins bearing complex-type oligosaccharide(s). Thus cTP-1 is a mixture of glycosylated and unglycosylated polypeptides.
Baumbach
G A
GA
Department of Animal Science, University of Tennessee, Knoxville 39701-1071.
Duby
R T
RT
Godkin
J D
JD
D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006031:Glycosylation; D006041:Goats; D007370:Interferon Type I; D007700:Kinetics; D008970:Molecular Weight; D009924:Organ Culture Techniques; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D014415:Tunicamycin / Q000494:pharmacology
D007370:Interferon Type I; D011257:Pregnancy Proteins; C020947:interferon tau; D014415:Tunicamycin