Electrophilic levuglandin E2-protein adducts bind glycine: a model for protein crosslinking.
Prevention by glycine of protein crosslinking which accompanies binding of levuglandin E2 (LGE2) is shown to involve binding of glycine with the protein-LGE2 adduct. With ovalbumin, the LGE2 adduct initially binds nearly 2 equivalents of glycine, but the capacity to bind glycine decreases with time reflecting a competition, inter alia, with crosslinking.
Jirousek
M R
MR
Department of Chemistry, Case Western Reserve University, Cleveland, Ohio 44106-2699.
Murthi
K K
KK
Salomon
R G
RG
D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D005998:Glycine / Q000378:metabolism*; D007700:Kinetics; D010047:Ovalbumin / Q000378:metabolism*; D011458:Prostaglandins E / Q000378:metabolism
D003432:Cross-Linking Reagents; D011458:Prostaglandins E; D010047:Ovalbumin; C047684:levuglandin E2; D005998:Glycine