title: Immunophenotypic and genotypic characterization of primary non-Hodgkin's lymphoma of the gastrointestinal tract.
abstract: Antigen receptor gene rearrangement studies have been applied to gastrointestinal (GI) lymphoid proliferations in only a limited number of cases, and their use and contribution to the diagnosis and characterization of GI lymphomas is unknown. We retrospectively studied 17 cases of primary GI lymphoma using fresh/frozen tissue with a combination of immunophenotypic and genotypic techniques. The vast majority of the neoplasms were B-cell lymphomas (88%) with rare T-cell tumors. The most common B-cell immunophenotype was IgM-kappa (40%), while five of the B-cell lymphomas (33%) lacked surface light chain immunoglobulin. Immunophenotypic evidence of histiocytic differentiation was not identified. Clonality was confirmed in 59% (10/17) of the neoplasms by immunophenotyping and 88% (15/17) by antigen receptor gene rearrangement studies. All of the 15 B-cell lymphomas (100%) demonstrated clonally rearranged immunoglobulin gene rearrangement. The two lymphomas with T-cell immunophenotypes did not demonstrate T-cell receptor beta-chain gene rearrangement. Antigen receptor gene rearrangement data can be useful and may even be necessary in certain cases for the proper classification and/or diagnosis of GI lymphoid proliferations.
authors.affiliation: Department of Pathology, University of Iowa Hospitals and Clinics, Iowa City.
authors.forename: M J
authors.initials: MJ
authors.lastname: Laszewski
authors.forename: D
authors.initials: D
authors.lastname: Kamat
authors.forename: J D
authors.initials: JD
authors.lastname: Kemp
authors.forename: J A
authors.initials: JA
authors.lastname: Goeken
authors.forename: F A
authors.initials: FA
authors.lastname: Mitros
authors.forename: C E
authors.initials: CE
authors.lastname: Platz
authors.forename: F R
authors.initials: FR
authors.lastname: Dick
mesh_terms: D000328:Adult; D000368:Aged; D001402:B-Lymphocytes; D002648:Child; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000235:genetics* / Q000276:immunology*; D015321:Gene Rearrangement / Q000276:immunology; D005838:Genotype; D006801:Humans; D016130:Immunophenotyping; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics* / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D013601:T-Lymphocytes