Isolation and restriction endonuclease analysis of a tetracycline resistance plasmid from Staphylococcus hyicus.
A plasmid of 4.550 kb, conferring resistance to tetracycline, was demonstrated in Staphylococcus hyicus cultures from piglets with exudative epidermidis. The plasmid-encoded properties were determined both by curing and interspecific protoplast transformation experiments. The tetracycline resistance (TET) plasmid, designated pST1, was characterized by restriction endonuclease analysis and a preliminary restriction map was constructed. The pST1 plasmid was demonstrated in 19 (57.6%) of 33 S. hyicus cultures by Southern blot hybridization. It was also detectable by electron microscopy.
Schwarz
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S
Institut für Bakteriologie und Immunologie der Justus-Liebig-Universität Giessen, F.R.G.
Blobel
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H
D000818:Animals; D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000032:analysis* / Q000302:isolation & purification / Q000648:ultrastructure; D004270:DNA, Circular / Q000032:analysis / Q000302:isolation & purification / Q000648:ultrastructure; D004587:Electrophoresis, Agar Gel; D004818:Epidermitis, Exudative, of Swine / Q000382:microbiology*; D008854:Microscopy, Electron; D011815:R Factors; D015183:Restriction Mapping; D013210:Staphylococcus / Q000187:drug effects / Q000235:genetics*; D013552:Swine; D013753:Tetracycline Resistance / Q000235:genetics*; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D004270:DNA, Circular