Isolation and characterization of human reg protein produced in Saccharomyces cerevisiae.
reg was originally identified as a gene expressed during the regeneration of insulin-producing pancreatic beta-cells of the rat. We built an expression vector containing human reg cDNA to drive Saccharomyces cerevisiae to synthesize the reg protein, and purified it from the culture medium. The 144-amino acid sequence of the recombinant protein was consistent with that deduced from the cDNA and genomic DNA sequence except that the signal sequence of 22 amino acids was eliminated, and the amino-terminal residue of the protein was pyroglutamic acid. The secondary structure of the reg protein was predicted by determination of the intramolecular cystine linkage and of alpha-helix and beta-sheet contents.
Itoh
T
T
Shionogi Research Laboratories, Shionogi & Co. Ltd., Osaka, Japan.
Tsuzuki
H
H
Katoh
T
T
Teraoka
H
H
Matsumoto
K
K
Yoshida
N
N
Terazono
K
K
Watanabe
T
T
Yonekura
H
H
Yamamoto
H
H
D000595:Amino Acid Sequence; D002135:Calcium-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D003553:Cystine; D004247:DNA / Q000235:genetics; D004220:Disulfides; D006801:Humans; D007515:Islets of Langerhans / Q000502:physiology*; D051847:Lithostathine; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D012038:Regeneration; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D002135:Calcium-Binding Proteins; D004220:Disulfides; D051847:Lithostathine; D009419:Nerve Tissue Proteins; C497617:REG1A protein, human; D011994:Recombinant Proteins; D003553:Cystine; D004247:DNA