Insertion of plasmids into the chromosome of Streptomyces griseofuscus.
The results demonstrate a general method for randomly integrating plasmids into the genome by a single crossover between a cloned DNA fragment and homologous DNA in the chromosome. The integrated plasmid is flanked by directly repeated copies of the cloned homologous DNA sequence. Two protocols, "replica plating" and "liquid transfer," yielded strains with integrated plasmids.
Larson
J L
JL
Lilly Corporate Center, Eli Lilly and Company, Indianapolis, Indiana 46285.
Hershberger
C L
CL
D002876:Chromosomes, Bacterial; D015723:Gene Library; D005821:Genetic Techniques; D010957:Plasmids; D011995:Recombination, Genetic; D015183:Restriction Mapping; D013302:Streptomyces / Q000235:genetics*