P190 BCR/ABL transcript in a case of Philadelphia-positive multiple myeloma.
Philadelphia positive multiple myeloma is a very rare event and, so far, no molecular data about the involvement of the BCR and C-ABL genes are available. We report here the case of a 64-year-old woman presenting with a typical multiple myeloma and a complex Philadelphia (Ph) chromosome that we investigated at a molecular level using conventional DNA techniques and the polymerase chain reaction (PCR). No rearrangement was observed within the major breakpoint cluster region (M-BCR) although she was found to have a P190 BCR/ABL hybrid transcript using PCR. As far as we know, this is the first description of a P190-type mRNA in a patient with a chronic lymphoid disorder. Since P190 is almost always associated in man with acute forms of hematological malignancies, this suggests that other factors may play a role in determining the phenotype of the disease.
Martiat
P
P
Department of Haematology, University of Louvain Medical School, Brussels, Belgium.
Mecucci
C
C
Nizet
Y
Y
Stul
M
M
Philippe
M
M
Cassiman
J J
JJ
Michaux
J L
JL
Van den Berghe
H
H
Sokal
G
G
D001483:Base Sequence; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D008875:Middle Aged; D008969:Molecular Sequence Data; D009101:Multiple Myeloma / Q000235:genetics / Q000378:metabolism*; D015345:Oligonucleotide Probes; D010677:Philadelphia Chromosome; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D014158:Transcription, Genetic
D004273:DNA, Neoplasm; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D016044:Fusion Proteins, bcr-abl