Radiobiological dose-response relationships by monitoring the ATP-concentration in L 1210 cells using 31P-NMR-spectroscopy.
In conventional radiobiology the survival fraction of cell cultures in the exponential phase is observed by the colony-forming ability, and the linear-quadratic model represents an adequate frame of the numerical adaptation. The measurement of the ATP-concentration of L 1210 cells by 31P-NMR-spectroscopy in dependence of the applied radiation dose can also be used to evaluate dose-response relationships. In particular, the beta-peak of the ATP is suitable to seize the chronological behavior of the cellular ATP-concentration. An access to RBE of a radiation quality is obtained by monitoring the ATP-concentration, and the combination treatment (irradiation and incubation of cisPlatinum) has also been studied with regard to the question of synergistic interactions.
Ulmer
W
W
FB Radiologie, Bereich Medizinphysik, St. Marienkrankenhaus Siegen, BRD.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D066298:In Vitro Techniques; D007939:Leukemia L1210; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000528:radiation effects*
D000255:Adenosine Triphosphate