title: [Cloning, structure and expression of the full-size lon gene in Escherichia coli coding for ATP-dependent La-proteinase].
abstract: Assembly of the full Escherichia coli K-12 lon gene from the EcoRI--SphI fragment of the bacterial DNA ("modified" gene) cloned and sequenced earlier and the PstI fragment of the same DNA containing 3'-terminal region of the lon gene has been performed. Both "modified" and full genes showed all phenotype properties of lon gene. The complete nucleotide sequence of the gene (2770 bp) coding for the 784 amino acid sequence of protease La was determined. Location of catalytically active serine, histidine and aspartic acid residues was suggested, and ATP-binding site found. The lon gene and protease La structures we found are compared with those described independently and differences observed are discussed.
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Amerik
authors.forename: V K
authors.initials: VK
authors.lastname: Antonov
authors.forename: N I
authors.initials: NI
authors.lastname: Ostroumova
authors.forename: T V
authors.initials: TV
authors.lastname: Rotanova
authors.forename: L G
authors.initials: LG
authors.lastname: Chistiakova
mesh_terms: D049069:ATP-Dependent Proteases; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins; D015870:Gene Expression; D005798:Genes, Bacterial; D006360:Heat-Shock Proteins; D008969:Molecular Sequence Data; D049070:Protease La; D011487:Protein Conformation; D015183:Restriction Mapping; D012697:Serine Endopeptidases / Q000737:chemistry / Q000235:genetics*
chemical_list: D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D049069:ATP-Dependent Proteases; D012697:Serine Endopeptidases; C483567:Lon protein, E coli; D049070:Protease La
vernacular_title: Klonirovanie, struktura i ékspressiia polnorazmernogo gena lon Escherichia coli, kodiruiushchego ATP-zavisimuiu La-proteinazu.