title: Sequence of a cDNA encoding human galactose-1-phosphate uridyl transferase.
abstract: We report a revised sequence of a cDNA that encodes a human galactose-1-phosphate uridyl transferase. The cDNA is 1295 bases in length and encodes a 43,000 Mr protein. The sequence was derived from a cDNA clone isolated from a transformed human lymphoblast cell line and amplified in a polymerase chain reaction. The revised sequence reveals a higher degree of amino acid conservation between the human enzyme and the homologous enzymes from Escherichia coli and yeast than was previously thought to exist.
authors.affiliation: Department of Pediatrics, Emory University, Atlanta, GA 30322.
authors.forename: J E
authors.initials: JE
authors.lastname: Flach
authors.forename: J K
authors.initials: JK
authors.lastname: Reichardt
authors.forename: L J
authors.initials: LJ
authors.lastname: Elsas
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D005694:UTP-Hexose-1-Phosphate Uridylyltransferase / Q000235:genetics*
chemical_list: D004274:DNA, Recombinant; D004247:DNA; D005694:UTP-Hexose-1-Phosphate Uridylyltransferase