Embryonic and adult neurones of the housefly (Musca domestica) in culture.
This paper provides a detailed description of the dissociation and maintenance in culture of cells derived from embryonic tissue and from the central nervous system of adult housefly, Musca domestica. Dissociation of embryonic tissue produced several cell types of which muscle and neurone-like cells predominated. These cells were morphologically distinct once development had started in vitro with muscle cells often producing rhythmic contractions. Neurones had small cell bodies (less than 10 mum) and showed extensive outgrowth of neurites. Neuronal cell bodies readily sealed onto patch pipettes and with pipette solutions containing nicotinic agonists, single channel currents could be recorded. With cell-attached patches, these currents were inward at the cell resting potential and reversed with depolarization. Channels with two different conductances could be seen in the majority of patches.
Harrison
J B
JB
Department of Zoology, University of Cambridge, UK.
Leech
C A
CA
Katz
J
J
Sattelle
D B
DB
D000818:Animals; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology / Q000196:embryology / Q000502:physiology; D004594:Electrophysiology; D006793:Houseflies / Q000166:cytology* / Q000502:physiology; D008564:Membrane Potentials / Q000502:physiology; D009474:Neurons / Q000166:cytology* / Q000502:physiology