Synthesis and characterization of a new substrate of Photinus pyralis luciferase: 4-methyl-D-luciferin.
A derivative of luciferin, 4-methyl-D-luciferin, was synthesized in low yield, and characterized by its ultraviolet and fluorescence spectra. The spectra of luciferin and its derivative are very similar. 4-Methyl-D-luciferin is a substrate of Photinus pyralis luciferase, and it causes light emission in luminometric assays.
Farace
C
C
CEMAGREF, Laboratoire de Biochimie, Lyon, France.
Blanchot
B
B
Champiat
D
D
Couble
P
P
Declercq
G
G
Millet
J L
JL
D000818:Animals; D049454:Firefly Luciferin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D007202:Indicators and Reagents / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D007313:Insecta; D008156:Luciferases / Q000378:metabolism*; D013379:Substrate Specificity
D007202:Indicators and Reagents; C066305:4-methylluciferin; D049454:Firefly Luciferin; D008156:Luciferases