title: [Epikeratoplasty without sutures using collagen IV lens in monkeys: description of the surgical technic].
abstract: The authors describe their original epikeratoplasty technic without sutures which is compatible with the use of collagen IV lens. They describe the first four observations of primates operated on using this technic. At first the epithelium is removed at the cornea center and a trepanation is made of 4 mm diameter and 0.1 mm depth. The bottom of the trepanation is then cut horizontally, and the periphery of the lens is put in the cornea stroma. Later the epithelium will recover the collagen lens. The lens is perfectly set in the cornea. We don't use any suture and so we avoid astigmatism and neovascularisation. The follow-up consisted of biomicroscopic examination photography, specular microscopy, pachymetry, photokeratoscopy (Nidek System) tonometry and histology. Clinical observance showed a perfect lens tolerance. The cornea is immediately transparent and within a week epithelial cells recovered the lens of three animals out of four. The photokeratoscopy study proved the important cornea refraction modification. This technic is reversible and the lens can be exchanged. A study of histology has begun and already shows a pluristratified epithelium. Further studies will test the biomaterial stability and ultra structural relations between the collagen IV lens and epithelial cells.
authors.affiliation: Centre Hospitalier Régional Universitaire de Grenoble.
authors.forename: P
authors.initials: P
authors.lastname: Albinet
authors.forename: J P
authors.initials: JP
authors.lastname: Romanet
authors.forename: M
authors.initials: M
authors.lastname: Mouillon
authors.forename: I
authors.initials: I
authors.lastname: Zerdab
authors.forename: P
authors.initials: P
authors.lastname: Gravagna
authors.forename: J L
authors.initials: JL
authors.lastname: Tayot
authors.forename: D
authors.initials: D
authors.lastname: Dupont
authors.forename: R
authors.initials: R
authors.lastname: Eloy
mesh_terms: D000818:Animals; D003094:Collagen; D003263:Contact Lenses, Hydrophilic; D016039:Corneal Transplantation / Q000379:methods*; D005500:Follow-Up Studies; D006801:Humans; D008252:Macaca fascicularis; D013997:Time Factors
chemical_list: D003094:Collagen
vernacular_title: Epikératoplastie sans suture avec lentille de Collagène IV chez le singe: description de la technique chirurgicale.