title: The freeze-drying of Cowdria ruminantium.
abstract: Lyophilized tissues of mice and blood of sheep, infected with either the Kümm, the Welgevonden or the Ball 3 stock of Cowdria ruminantium, remain infective to mice and sheep after storage at 4 degree C for 90 days. Freeze-dried tissues stored at -18 degrees C and -28 degrees C are still infective after 6 months and 2 years, respectively.
authors.affiliation: Veterinary Research Institute, Onderstepoort.
authors.forename: J L
authors.initials: JL
authors.lastname: du Plessis
authors.forename: L
authors.initials: L
authors.lastname: van Gas
authors.forename: F J
authors.initials: FJ
authors.lastname: Labuschagne
authors.forename: S
authors.initials: S
authors.lastname: Wijma
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines; D002417:Cattle; D005612:Freeze Drying / Q000379:methods; D006357:Heartwater Disease / Q000517:prevention & control*; D008297:Male; D051379:Mice; D012287:Rickettsiaceae / Q000276:immunology*; D012756:Sheep; D013696:Temperature; D013997:Time Factors; D014611:Vaccination / Q000662:veterinary
chemical_list: D001428:Bacterial Vaccines