[Electron microscopic cytochemistry of pseudo-Chediak-Higashi granules in 5 cases of AML].
Blasts from 5 cases of AML with pseudo-Chediak-Higashi granules were examined ultrastructurally and histocytochemically using peroxidase, acid phosphatase, high iron diamine (HID) and periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) stainings. Pseudo-Chediak-Higashi granules, which appeared as vacuole-like inclusions by light microscopy, generally contained electron-lucent materials. All pseudo-Chediak-Higashi granules were, positive for peroxidase but some were negative for acid phosphatase. Pseudo-Chediak-Higashi granules were HID positive, indicating that they contained sulfated glycoconjugates. Glycogen-like particles were observed in the pseudo-Chediak-Higashi granules with the PA-TCH-SP method, as occasionally observed in granules in drug resistant ALL blasts. In conclusion, the contents of pseudo-Chediak-Higashi granules, which seems to be formed by fusion of small granules, differed from those of normal azurophillic granules.
Mizushima
W
W
Second Department of Pediatrics, Dokkyo University School of Medicine.
Eguchi
M
M
Sakakibara
H
H
Sugita
K
K
Furukawa
T
T
Kanagawa
M
M
Suda
T
T
Yoshida
M
M
Miura
Y
Y
Matsui
J
J
D000328:Adult; D000368:Aged; D002609:Chediak-Higashi Syndrome / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D002648:Child; D006651:Histocytochemistry; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged