title: Immunohistochemical localization of vimentin and S-100 antigen in small acoustic tumors and adjacent cochlear nerves.
abstract: To clarify the involvement of cochlear nerves in small acoustic tumors, we used immunoperoxidase techniques to determine the presence and distribution of vimentin and S-100 antigens in two acoustic tumor specimens and a transected vestibular nerve. Schwann cells and acoustic tumor cells failed to react positively with monoclonal antibody to vimentin. Reaction was observed in mesenchymal-appearing cells within both the normal nerve and the acoustic tumors, predominantly in association with blood vessels. Normal schwann cells and acoustic tumor cells reacted with polyclonal antibody to S-100 antigen with a similar, uniform distribution. Mesenchymal-appearing cells did not react with antibody to S-100. Immunostaining of a vestibular nerve from a Meniere's disease patient, used as a control, did not differ significantly from nerves adjacent to acoustic tumors. Because tumor cells and normal schwann cells stained similarly with antibody to S-100, it was not possible to establish with certainty if tumor cells invaded adjacent nerves.
authors.affiliation: Department of Otology and Laryngology, Kasturba Medical College Hospital, Manipal, India.
authors.forename: G K
authors.initials: GK
authors.lastname: Hebbar
authors.forename: M J
authors.initials: MJ
authors.lastname: McKenna
authors.forename: F H
authors.initials: FH
authors.lastname: Linthicum
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis / Q000276:immunology; D003056:Cochlear Nerve / Q000276:immunology; D003390:Cranial Nerve Neoplasms / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D009464:Neuroma, Acoustic / Q000737:chemistry* / Q000276:immunology; D009418:S100 Proteins / Q000032:analysis*; D012583:Schwann Cells / Q000737:chemistry* / Q000276:immunology / Q000648:ultrastructure; D014725:Vestibular Nerve / Q000276:immunology; D014746:Vimentin / Q000032:analysis* / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; D009418:S100 Proteins; D014746:Vimentin