Simultaneous immunoenzymometric assay for antibodies against human interleukin-2 and human serum albumin in rat serum.
A simultaneous immunoenzymometric assay for anti-human interleukin-2 antibody and anti-human serum albumin antibody in rat serum was developed. Two antigen-immobilized polystyrene balls were immersed in a diluted serum sample in an assay tube and then the antibodies on the balls were made to react with a horseradish peroxidase labelled anti-rat IgG antibody in the same tube after washing. The enzyme activity of each ball was measured by fluorometry. Not only were the sensitivity (70 ng/ml each), assay recovery (100-101%), and precision (C.V. = 5-13%) comparable to those of conventional immunoenzymometric assays using one antigen-immobilized ball but the assay was also much more feasible for mass routine assays. Thus, conventional immunoassays can be replaced by this convenient simultaneous method.
Kominami
G
G
Shionogi Research Laboratories, Shionogi & Co., Ltd., Osaka, Japan.
Kawamoto
H
H
Murai
Y
Y
Kono
M
M
D000818:Animals; D000906:Antibodies / Q000097:blood*; D006801:Humans; D007124:Immunoenzyme Techniques; D007376:Interleukin-2 / Q000276:immunology*; D008297:Male; D051381:Rats; D011994:Recombinant Proteins / Q000276:immunology; D015203:Reproducibility of Results; D012709:Serum Albumin / Q000276:immunology*
D000906:Antibodies; D007376:Interleukin-2; D011994:Recombinant Proteins; D012709:Serum Albumin