Development of a high performance liquid chromatography method for the simultaneous measurement of prednisone and prednisolone.
An improved high performance liquid chromatographic assay (HPLC) is presented for the quantitative determination of prednisone and its main metabolite prednisolone. This HPLC assay, employing a UV-detector and dexamethasone as an internal standard, provides a selective and sensitive determination of glucocorticoids in plasma. The advantage of this method is the possibility of simultaneously measuring exogenic and endogenic glucocorticoids. The lower limit of quantification is 5 ng/ml.
Huber
F
F
Analytical Research Centre of the LAB GmbH, Neu Ulm, Fed. Rep. of Germany.
Wiedemann
M
M
Heinrich
G
G
Salama
Z
Z
Jaeger
H
H
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D011239:Prednisolone / Q000097:blood* / Q000737:chemistry; D011241:Prednisone / Q000097:blood* / Q000737:chemistry; D011786:Quality Control; D013056:Spectrophotometry, Ultraviolet
D011239:Prednisolone; D011241:Prednisone