[The use of a molecular hybridization method for studying the enterotoxigenic properties of Salmonella].
The occurrence of the tox gene among 320 Salmonella strains of 23 serovars, differing in their origin, sensitivity to antibiotics, the presence of R-plasmids and a number of biochemical properties, has been studied by the method of DNA-DNA hybridization in situ. Essential differences in the occurrence of the tox gene have been detected both among S. typhimurium hospital strains and strains isolated in sporadic diseases, from the environment, from animals and among salmonellae belonging to different serovars. The direct correlation between the presence of the enterotoxigenicity gene and plasmids controlling resistance to antibiotics in Salmonella strains has been established. The expediency of using the method of gene probing for the study of the enterotoxigenic properties of salmonellae has been substantiated.
Blokhina
I N
IN
Brusnigina
N F
NF
Skoblo
L E
LE
Mazepa
V N
VN
Barysheva
N N
NN
D000818:Animals; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics*; D004274:DNA, Recombinant / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D004768:Enterotoxins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D005821:Genetic Techniques; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D012475:Salmonella / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D012703:Serotyping
D015342:DNA Probes; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004768:Enterotoxins
Primenenie metoda molekuliarnoĭ gibridizatsii dlia izucheniia énterotoksigennykh svoĭstv sal'monell.