title: Isolation of indigenous larvicidal microbial control agents of mosquitos: the Malaysian experience.
abstract: A nationwide screening program searching for microbial control agents of mosquitos was initiated in Malaysia in 1986. A total of 725 samples were collected and 2,394 bacterial colonies were isolated and screened for larvicidal activity. From such screening, 20 Bacillus thuringiensis, 6 B. sphaericus, 1 Clostridium bifermentans and 2 Pseudomonas pseudomallei larvicidal isolates were obtained. Of these, a new B. thuringiensis named as subspecies malaysianensis was found, while the C. bifermentans was also a new anaerobe individualized as serovar malaysia. It was concluded that this screening program was highly successful.
authors.affiliation: Division of Medical Entomology, Institut for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia.
authors.forename: H L
authors.initials: HL
authors.lastname: Lee
authors.forename: P
authors.initials: P
authors.lastname: Seleena
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000302:isolation & purification*; D001681:Biological Assay; D003013:Clostridium / Q000302:isolation & purification*; D004783:Environmental Microbiology; D007814:Larva; D008296:Malaysia; D008828:Microbiological Techniques; D009032:Mosquito Control / Q000379:methods*; D010572:Pest Control, Biological / Q000379:methods*; D011549:Pseudomonas / Q000302:isolation & purification*