title: Effect of cytochrome P-450 inhibition and stimulation on intensity of polyethylene degradation in microsomal fraction of mouse and rat livers.
abstract: Polyethylene (PE) is degraded in microsomal fractions of mouse and rat livers with the formation of carbonyl groups. Infrared spectroscopy demonstrated formation of predominantly ketone groups and to a lesser degree ester and aldehyde groups. The inhibition and stimulation of cytochrome P-450 in mouse livers affected the formation of oxidative groups on PE. Phenobarbital doses of 3 x 0.05 mg per mouse increased the concentration of cytochrome P-450 and ketone groups on PE, whereas the vaccine Propionibacterium acnes (0.5 mg) and its pyridine fraction (0.5 and 1 mg) had the opposite effect. The coherence of cytochrome P-450 with oxidative changes on PE is compared and discussed with findings on implants in man.
authors.affiliation: Laboratory of Microbiology, Electrical Testing Institute, Praha 8, Czechoslovakia.
authors.forename: M
authors.initials: M
authors.lastname: Beranová
authors.forename: R
authors.initials: R
authors.lastname: Wasserbauer
authors.forename: D
authors.initials: D
authors.lastname: Vancurová
authors.forename: M
authors.initials: M
authors.lastname: Stifter
authors.forename: J
authors.initials: J
authors.lastname: Ocenásková
authors.forename: M
authors.initials: M
authors.lastname: Mára
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000494:pharmacology; D001672:Biocompatible Materials; D001673:Biodegradation, Environmental; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000502:physiology*; D004731:Endotoxins / Q000494:pharmacology; D004926:Escherichia coli; D005260:Female; D051379:Mice; D008862:Microsomes, Liver / Q000502:physiology*; D010084:Oxidation-Reduction; D010634:Phenobarbital / Q000494:pharmacology; D011095:Polyethylenes; D011425:Propionibacterium acnes / Q000276:immunology; D019736:Prostheses and Implants; D051381:Rats; D015164:Vaccines, Inactivated
chemical_list: D001428:Bacterial Vaccines; D001672:Biocompatible Materials; D065607:Cytochrome P-450 Enzyme Inhibitors; D004731:Endotoxins; D011095:Polyethylenes; D015164:Vaccines, Inactivated; D003577:Cytochrome P-450 Enzyme System; D010634:Phenobarbital