Mutational analysis of enhancer domains responsive to trans-activation by the X gene of human hepatitis B virus.
The X gene product of human hepatitis B virus (HBV) trans-activates the HBV enhancer. In order to identify domains responsive to trans-activation by the X gene, we introduced a series of mutations into the HBV enhancer and assayed the enhancer activities in the presence of the X gene product. Our results suggest that the EP domain of the enhancer is essential for trans-activation by the X gene.
Goodarzi
G
G
Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, Springfield.
Ohno
H
H
Adams
R
R
Darabi
A
A
Tewari
A
A
Watabe
M
M
Watabe
K
K
D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003001:Cloning, Molecular; D004279:DNA, Viral; D004742:Enhancer Elements, Genetic; D004926:Escherichia coli / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016366:Open Reading Frames; D015534:Trans-Activators / Q000502:physiology*; D014407:Tumor Cells, Cultured; D054334:Viral Regulatory and Accessory Proteins
D004279:DNA, Viral; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; D015500:Chloramphenicol O-Acetyltransferase