title: Transcription of feline calicivirus RNA.
abstract: We report here the cloning and 3' sequence determination of feline calicivirus strain F9. Subcloning the 3' terminus enabled the production of strand specific probes for RNA synthesis. We extend the number of virus specific RNAs detected intracellularly to 8, and show that numbers 1-5 are represented as negative strands which may serve as templates in the synthesis of these RNAs.
authors.affiliation: Division of Virology, School of Pathological Sciences, University of Newcastle upon Tyne, New Medical School, England.
authors.forename: M J
authors.initials: MJ
authors.lastname: Carter
mesh_terms: D000818:Animals; D001483:Base Sequence; D002139:Caliciviridae / Q000235:genetics* / Q000302:isolation & purification; D002460:Cell Line; D003001:Cloning, Molecular; D015342:DNA Probes; D004279:DNA, Viral; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis*; D015183:Restriction Mapping; D014158:Transcription, Genetic
chemical_list: D015342:DNA Probes; D004279:DNA, Viral; D012367:RNA, Viral