title: Long term culture of rat soleus muscle in vitro. Its effects on glucose utilization and insulin sensitivity.
abstract: Rat soleus muscle strips cultured for 24 h in medium 199 were well preserved in terms of electron microscopy; ATP and creatine phosphate concentrations; rates of glucose utilization, glycogen and protein synthesis, and effects of insulin thereon. Culture led to modest changes in fluid spaces and intracellular (K+); increased basal glucose utilization up to two-fold; had no effect on the maximum response to insulin; and had no effect on sensitivity to insulin except in the presence of adenosine deaminase. Thus in vitro neither denervation nor absence of insulin had any marked effects in 24 h to decrease responses to insulin.
authors.affiliation: Nuffield Department of Clinical Biochemistry, John Radcliffe Hospital, Oxford, UK.
authors.forename: P B
authors.initials: PB
authors.lastname: Stace
authors.forename: D R
authors.initials: DR
authors.lastname: Marchington
authors.forename: A L
authors.initials: AL
authors.lastname: Kerbey
authors.forename: P J
authors.initials: PJ
authors.lastname: Randle
mesh_terms: D000243:Adenosine Deaminase / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D005947:Glucose / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009132:Muscles / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009924:Organ Culture Techniques / Q000379:methods; D010725:Phosphocreatine / Q000378:metabolism; D051381:Rats; D012016:Reference Values
chemical_list: D007328:Insulin; D010725:Phosphocreatine; D000255:Adenosine Triphosphate; D006003:Glycogen; D007770:L-Lactate Dehydrogenase; D000243:Adenosine Deaminase; D005947:Glucose