Nucleotide sequence of the G6-amylase gene from alkalophilic Bacillus sp. H-167.
The nucleotide sequence of the G6-amylase gene from alkalophilic Bacillus sp. H-167 was determined. The open reading frame of the gene consisted of 2865 base pairs, encoding 955 amino acids. The NH2-terminal amino acid sequence analysis of the G6-amylase indicated that the enzyme had a single peptide of 33 amino acid residues and the mature enzyme was composed of 922 amino acids, giving a molecular mass of 102,598. Identity of the NH2-terminal amino acid sequences among each component of the multiform G6-amylase suggested the proteolytic processing of the COOH-terminal side of the enzyme. The DNA sequence and the deduced amino acid sequence of the G6-amylase gene showed no homology with those of other bacterial alpha-amylases although the consensus amino acid sequences of the active center were well conserved.
Shirokizawa
O
O
Superbugs Project, Research Development Corporation of Japan, Tokyo.
Akiba
T
T
Horikoshi
K
K
D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D000516:alpha-Amylases / Q000235:genetics; D000520:alpha-Glucosidases / Q000235:genetics*
D004269:DNA, Bacterial; D000516:alpha-Amylases; D000520:alpha-Glucosidases; C065485:glucan 1,4-alpha-maltohexaosidase