A synthetic peptide elicits antibody reactive with the native duck hepatitis B virus pre-S protein.
Synthetic peptides P37-49 and P63-79, derived from the pre-S region of duck hepatitis B virus (DHBV), have been synthesized. Only P37-49 was reactive with rabbit anti-DHBs/pre-S antibodies by radioimmunoprecipitation. Antiserum prepared against P37-47 reacted with a 35K polypeptide of native DHBs/pre-S by immunoblotting. It is concluded that P37-49 (MGQHPAKSMDVRR) mimics one of the epitopes of the DHBV pre-S antigen.
Wen
Y M
YM
Department of Microbiology, Shanghai Medical University, People's Republic of China.
Xu
Y Y
YY
Zhang
W
W
Liu
Y Z
YZ
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D006514:Hepatitis B Surface Antigens / Q000138:chemical synthesis / Q000276:immunology*; D015611:Hepatitis B Virus, Duck / Q000276:immunology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D011233:Precipitin Tests; D011498:Protein Precursors / Q000138:chemical synthesis / Q000276:immunology*; D011817:Rabbits
D006514:Hepatitis B Surface Antigens; D010455:Peptides; D011498:Protein Precursors; C050806:presurface protein 1, hepatitis B surface antigen; C050807:presurface protein 2, hepatitis B surface antigen