[Immune responses to articular cartilage reconstruction using chondrocytes allograft transplant].
Epiphyseal cartilage grown in vitro was transplanted using fibrin clot into a cartilage defect produced on the femoral articular surface of the knee joints in white New Zealand rabbits 5-6 months of age. The transplantation was successful for two weeks in 64% and for 3-24 weeks in 26%. Almost dead transplanted tissues were replaced by fibrous tissues to preserve the joint function. Immunological studies such as counting of inflammatory cells in the joint fluid, evaluation of the anti-allo antibody titer by passive hemagglutination inhibition, immunofluorescence studies, and assay of cytolytic reaction by 51Cr labelled allogenic target cells suggested that the transplanted cartilage began to degenerate from two or three weeks after the transplantation partially due to humoral immunity but more importantly on account of cellular immunity.
Yoshinao
M
M
Department of Orthopaedic Surgery, Shimane Medical School, Japan.
D000818:Animals; D000917:Antibody Formation; D002357:Cartilage Diseases / Q000276:immunology / Q000601:surgery*; D006132:Growth Plate / Q000637:transplantation*; D007111:Immunity, Cellular; D011817:Rabbits