Brown adipose tissue thermogenesis is activated by electrical and chemical (L-glutamate) stimulation of the ventromedial hypothalamic nucleus in cold-acclimated rats.
Experiments were conducted to determine if both electrical and chemical stimulation of the ventromedial hypothalamic nucleus (VMH) could activate brown adipose tissue (BAT) thermogenesis. Age-matched, room-acclimated (21 degrees C) and cold-acclimated (4 degrees C for 3 weeks prior to testing) male Sprague-Dawley rats were given unilateral electrical or chemical stimulation to the VMH by way of a 'chemotrode apparatus'. The devised 'chemotrode' allowed both electrical stimulation (insulated piano wire stimulating electrode) and chemical stimulation (23 gauge stainless steel intracranial cannula of equal length) to be performed at the same VMH site using a common 19 gauge stainless steel outer guide tube. The first unilateral VMH electrical stimulation (0.5 ms pulse, 50 Hz and 120 microA for 30 s) caused no significant rise in interscapular brown adipose tissue temperature (TIBAT) colonic (Tc) or tail surface temperatures (Tt), compared to respective prestimulation control values in rats acclimated to 21 degrees C. In the 4 degrees C-acclimated group the first VMH electrical stimulation caused a significant rise in IBAT temperature. L-Glutamate administration to the same VMH site (60 nmol in 600 nl volume) also caused a significant increase in IBAT temperature in the 4 degrees C but not the 21 degrees C-acclimated rats. The rise in IBAT temperature following the L-glutamate injection to the 4 degrees C-acclimated group was similar to that found following the first electrical stimulation to this group. Interestingly, a second unilateral electrical stimulation of the VMH to 4 degrees C-acclimated rats could not evoke a similar increase in IBAT temperature suggesting that overall L-glutamate was acting in vivo as an excitotoxin.(ABSTRACT TRUNCATED AT 250 WORDS)
Halvorson
I
I
Department of Physiology, University of Saskatchewan, Saskatoon, Canada.
Gregor
L
L
Thornhill
J A
JA
D000064:Acclimatization / Q000502:physiology*; D002001:Adipose Tissue, Brown / Q000502:physiology*; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001833:Body Temperature Regulation / Q000187:drug effects / Q000502:physiology*; D003080:Cold Temperature; D004558:Electric Stimulation; D005971:Glutamates / Q000494:pharmacology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical; D013564:Sympathetic Nervous System / Q000502:physiology; D014697:Ventromedial Hypothalamic Nucleus / Q000187:drug effects / Q000502:physiology*
D005971:Glutamates