title: Administration of pig relaxin to beef heifers 4 or 7 days pre partum.
abstract: Crossbred beef heifers (N = 36) were assigned to one of three treatment groups: untreated controls (C; N = 15); Group R4, treated with pig relaxin (1.0 mg i.m.) 4 days pre partum (N = 11); or Group R7, treated with pig relaxin (1.0 mg i.m.) 7 days pre partum (N = 10). Bioactivity of the pig relaxin (UMC-R-P8) was determined by the mouse interpubic ligament assay to be greater than or equal to 3000 U/mg, both before and after the experiment was conducted. Peripheral serum immunoreactive relaxin values were 7.5, 3.4, 2.5, and 1.5 ng/ml at 2, 4, 6 and 8 h after injection of relaxin, respectively. Gestation lengths were 282.9 +/- 1.1, 285.5 +/- 1.3 and 285.6 +/- 1.5 days for Groups C, R4 and R7 (C vs R4 + R7; P congruent to 0.08). Calving difficulty score (1 to 4) tended to be greater (P congruent to 0.08) for Group R4 and R7 heifers (C vs R4 + R7; 1.3 +/- 0.24 vs 1.75 +/- 0.28 + 2.04 +/- 0.32), but the incidences of dystocia and retained placentae were not influenced by treatment (P greater than or equal to 0.10). The mean concentration and concentration profile of daily serum progesterone, oestradiol-17 beta, dihydroprostaglandin F-2 alpha and relaxin were not affected by treatment from 6 days pre partum through 2 days post partum. Cervical diameter, cervical softness score, pelvic measurements, and vulva opening length during the periparturient period were not affected (P greater than or equal to 0.10) by treatment, but all of these characteristics changed over time (P less than or equal to 0.01), relative to calving. We conclude that i.m. administration of pig relaxin (greater than or equal to 3000 U) does not effectively alter periparturient characteristics of beef heifers. Discrepancies between these results and those reported for intracervical administration cannot be readily explained.
authors.affiliation: Department of Animal Sciences, University of Missouri, Columbia 65211.
authors.forename: R W
authors.initials: RW
authors.lastname: Caldwell
authors.forename: R A
authors.initials: RA
authors.lastname: Bellows
authors.forename: J A
authors.initials: JA
authors.lastname: Hall
authors.forename: R V
authors.initials: RV
authors.lastname: Anthony
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D015237:Dinoprost / Q000031:analogs & derivatives / Q000097:blood; D004305:Dose-Response Relationship, Drug; D004420:Dystocia / Q000517:prevention & control*; D004958:Estradiol / Q000097:blood; D005260:Female; D005865:Gestational Age; D007273:Injections, Intramuscular; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D012065:Relaxin / Q000008:administration & dosage* / Q000097:blood / Q000627:therapeutic use; D013552:Swine
chemical_list: C008228:15-keto-13,14-dihydroprostaglandin F2alpha; D011374:Progesterone; D004958:Estradiol; D012065:Relaxin; D015237:Dinoprost