Distribution of the early light-inducible protein in the thylakoids of developing pea chloroplasts.
The distribution of the early light-inducible protein (ELIP) of pea (Pisum sativum) between grana and stroma thylakoids was studied. An antibody raised against a bacterial-expressed fusion protein containing ELIP sequences was used. Illumination of dark-grown pea seedlings causes an accumulation of the ELIP in the thylakoid membranes with a maximum level at 16 h. During continuous illumination exceeding 16 h the level decreases again. The fractionation of thylakoid membranes of 48-h-illuminated pea seedlings in grana and stroma thylakoids reveals that there is no uniform distribution of ELIP in the thylakoids. Rather 60-70% of ELIP was found in the stroma thylakoids and 30-40% in the grana thylakoids. This distribution is in accordance with that of photosystem I but not with that of photosystem II. After Triton-X-100 solubilization almost all ELIP is found in the photosystem-I-containing fraction. This also supports an association of ELIP with photosystem I.
Cronshagen
U
U
Institut für Botanik, Universität Hannover, Federal Republic of Germany.
Herzfeld
F
F
D000595:Amino Acid Sequence; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D002736:Chloroplasts / Q000378:metabolism*; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D007887:Fabaceae; D007700:Kinetics; D008027:Light; D008969:Molecular Sequence Data; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism*; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D010946:Plants, Medicinal; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012689:Sequence Homology, Nucleic Acid
D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D011993:Recombinant Fusion Proteins