title: Time of low-dose acetylsalicylic acid administration influences in vivo platelet function and thrombus formation following arteriotomy and intimectomy; an experimental study in small arteries of rabbits.
abstract: To investigate if low-dose acetylsalicylic acid (ASA), 4 mg/kg b.w., infused peroperatively or 10 hours preoperatively has antithrombotic effects, the central arteries of rabbit ears were prepared and 32P-labeled platelets injected. Arteriotomy and intimectomy were performed and blood flow was restored. Bleeding times at the sites of arteriotomy/intimectomy, in vivo accumulations of isotope-labeled platelets, amounts of red thrombotic material, and patency were recorded. Bleeding times following arterial puncture and the effect of ASA on thromboxane production were studied separately. Ten hours after ASA administration, bleeding times were shortened at the sites of arteriotomy/intimectomy but were prolonged following arterial puncture. Platelet accumulations were lower in patent vessels in this group than in an untreated control group. Peroperative ASA treatment increased but treatment 10 hours prior to blood flow restoration did not significantly affect the number of occlusions. Thromboxane production in ASA-treated rabbits is largely inhibited even 14 hours after administration.
authors.affiliation: Department of Experimental Research, Malmö General Hospital, University of Lund, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Salemark
authors.forename: J B
authors.initials: JB
authors.lastname: Wieslander
authors.forename: P
authors.initials: P
authors.lastname: Dougan
authors.forename: B
authors.initials: B
authors.lastname: Arnljots
mesh_terms: D000818:Animals; D001158:Arteries / Q000473:pathology / Q000601:surgery; D001241:Aspirin / Q000008:administration & dosage / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects; D001792:Blood Platelets / Q000737:chemistry / Q000187:drug effects*; D004431:Ear, External / Q000098:blood supply*; D005260:Female; D008297:Male; D008855:Microscopy, Electron, Scanning; D010973:Platelet Adhesiveness / Q000187:drug effects*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D011817:Rabbits; D013927:Thrombosis / Q000503:physiopathology*; D013929:Thromboxane B2 / Q000097:blood; D013997:Time Factors; D014654:Vascular Patency
chemical_list: D010975:Platelet Aggregation Inhibitors; D013929:Thromboxane B2; D001241:Aspirin