title: Complementation of coat protein-defective TMV mutants in transgenic tobacco plants expressing TMV coat protein.
abstract: Transgenic tobacco plants (Nicotiana tabacum cv. Xanthi) which express tobacco mosaic virus (TMV) U1 strain coat protein (CP) can complement both the assembly and the long-distance spread of CP-defective (DT1) or coat proteinless (DT1G) mutants of TMV. Both mutants arose spontaneously from PM2 and exist only as unencapsidated RNA in the inoculated leaves of control tobacco plants, where they are unable to form virus particles or to spread systemically. TMV CP expressed in transgenic tobacco plants [CP+ line 3404; P. Powell Abel, R. S. Nelson, B. De, N. Hoffman, S. G. Rogers, R. T. Fraley, and R. N. Beachy, 1986, Science 232, 738-743] was able to package some of either mutant viral RNA into TMV-like particles in vivo and resulted in the long-range spread of infection. In vivo encapsidated DT1 RNA was recovered and reinoculated onto control or new CP+ transgenic tobacco plants. Localized infection of control plants confirmed that no RNA recombination or reversion of the mutant RNA to wild-type had occurred during passage in the first CP+ plant. In contrast, encapsidated DT1 RNA was unable to produce even local infection in CP+ transgenic plants confirming that CP-mediated protection operates during the early stages of virus infection, including particle uncoating. By positive complementation, these results also confirm that TMV CP is required for the long-distance spread of infection.
authors.affiliation: Department of Virus Research, John Innes Institute, United Kingdom.
authors.forename: J K
authors.initials: JK
authors.lastname: Osbourn
authors.forename: S
authors.initials: S
authors.lastname: Sarkar
authors.forename: T M
authors.initials: TM
authors.lastname: Wilson
mesh_terms: D015152:Blotting, Northern; D002213:Capsid / Q000235:genetics*; D005816:Genetic Complementation Test; D010947:Plants, Toxic; D012367:RNA, Viral / Q000235:genetics; D014026:Nicotiana / Q000235:genetics / Q000382:microbiology; D014027:Tobacco Mosaic Virus / Q000235:genetics* / Q000254:growth & development; D014779:Virus Replication
chemical_list: D012367:RNA, Viral