Decreased activity of the sodium-calcium exchanger in tail artery of stroke-prone spontaneously hypertensive rats.
The ability of the Na-Ca exchanger to modify vascular relaxation was studied in rings isolated from tail arteries of stroke-prone spontaneously hypertensive rats (SHRSP) and normotensive Wistar-Kyoto rats (WKY). The arteries were contracted with norepinephrine (NE) 1 microM and after stabilization they were transferred to a Ca-free physiological salt solution still in presence of NE. The time to 50% relaxation (T-50) in these conditions was significantly greater in SHRSP (78 +/- 7 s) than in WKY (50 +/- 7 s). When the calcium pump was stopped with vanadate (VAN), the Ca uptake by the sarcoplasmic reticulum with ryanodine (RY) and the Na-Ca exchanger with a Na-free PSS, the relaxation was slowed (T-50 increased to 198 +/- 16 s in SHRSP and to 162 +/- 14 s in WKY). Releasing the Na-Ca exchanger only (i.e. still with VAN and RY but with normal Na in the bath) the T-50 for relaxation in Ca-free PSS was, in WKY, nearly as fast as in control conditions (54 +/- 8 s). However, the Na-Ca exchanger in SHRSP was not so effective, and the T-50 for relaxation was slower than in control conditions (122 +/- 10 s). We conclude that the activity of the Na-Ca exchanger is depressed in tail arteries of SHRSP. This abnormality in resistance vessels, would contribute to the enhanced vascular tone present in hypertension.
Thompson
L E
LE
Department of Physiology, University of Michigan, Ann Arbor.
Rinaldi
G J
GJ
Bohr
D F
DF
D000818:Animals; D001158:Arteries / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D002561:Cerebrovascular Disorders / Q000150:complications / Q000378:metabolism*; D006973:Hypertension / Q000150:complications / Q000378:metabolism*; D009126:Muscle Relaxation; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D011919:Rats, Inbred Strains; D019831:Sodium-Calcium Exchanger; D013623:Tail / Q000098:blood supply* / Q000378:metabolism
D002352:Carrier Proteins; D019831:Sodium-Calcium Exchanger; D002118:Calcium