[DNA extraction from body fluids of cadavers].
DNA of 1 ml Ascites, bile, liquor, pleural-exsudate, pericardial-fluid, synovial-fluid, urine and vitreous humor of sixteen bodies of either sex was isolated. Applying dot-blot-hybridisation black spots were obtained by using total human DNA as DNA-probe (A), if the origin is human. Our results showed, that body-fluids of dead persons contain human DNA, mainly. After rehybridisation with a sex-specific DNA-probe (Y) (pJA 1143) a determination of sex can be performed.
Josephi
E
E
Institut für Rechtsmedizin, Universität München.
Braito
U
U
Tutsch-Bauer
E
E
D001826:Body Fluids / Q000737:chemistry*; D004247:DNA / Q000032:analysis* / Q000235:genetics; D015342:DNA Probes; D005260:Female; D006801:Humans; D008297:Male; D010641:Phenotype; D012732:Sex Determination Analysis
D015342:DNA Probes; D004247:DNA
DNA-Extraktion aus Körperflüssigkeiten von Verstorbenen.