Establishment of a macrophagelike cell line derived from U-937, human histiocytic lymphoma, grown serum-free.
A human macrophagelike cell line which grows in serum-free medium was established from a histiocytic lymphoma cell line, U-937. U-937 cells failed to differentiate into macrophagelike cells in serum-free medium plus 12-O-tetradecanoyl phorbol 13-acetate (TPA). Fibronectin and albumin in serum were necessary for differentiation of U-937 cells into macrophagelike cells in enriched RDF medium supplemented with insulin, transferrin, ethanolamine, selenite, egg yolk lipoprotein (eRDF-ITESL medium). The established cell line exhibited several characteristic properties of macrophage such as nitroblue tetrazolium reduction, phagocytic and alpha-naphthylbutyrate-esterase activities, and tumor necrosis factor and interleukin 1 production. At present the cells have been continuously maintained in eRDF-ITESL medium through over 150 passages.
Kong
Z L
ZL
Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Miwa
M
M
Murakami
H
H
Shinohara
K
K
D001769:Blood; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003470:Culture Media; D005353:Fibronectins / Q000494:pharmacology; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis; D016403:Lymphoma, Large B-Cell, Diffuse / Q000473:pathology; D008264:Macrophages / Q000166:cytology*; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010587:Phagocytosis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D003470:Culture Media; D005353:Fibronectins; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D009580:Nitroblue Tetrazolium; D013755:Tetradecanoylphorbol Acetate