[Quantitation of intact form of complement protein B and its fragment Ba in human serum by radioimmunoassay].
A sensitive solid-phase radioimmunoassay for intact complement protein B and its smaller fragment Ba is described which quantitates human B and Ba to 30 ng/ml. The assay was proved to be useful to evaluate the activation of the alternative pathway of the complement system by measuring intact B and Ba in normal human sera activated with zymosan. In sera from patients with systemic lupus erythematosus (SLE), the level of intact B was decreased and that of Ba was increased in the active phase, and the level of Ba was increased though intact B which was within normal limits in the inactive phase. The present result suggests that the activation of the alternative pathway is occurring both in the active and inactive phase in SLE, and the measurement of intact B and Ba concentration in pathological sera would be useful to evaluate the activation of the alternative complement pathway.
Ueda
A
A
First Department of Internal Medicine, Kyushu University, Fukuoka-City.
Nagasawa
K
K
Tsukamoto
H
H
Kushimoto
K
K
Ishii
Y
Y
Yamauchi
Y
Y
Tada
Y
Y
Niho
Y
Y
Kusaba
T
T
Volanakis
J E
JE
D000328:Adult; D000911:Antibodies, Monoclonal; D003179:Complement C3b / Q000032:analysis*; D011415:Complement Factor B / Q000032:analysis*; D003170:Complement Pathway, Alternative; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D010446:Peptide Fragments / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods*
D000911:Antibodies, Monoclonal; D010446:Peptide Fragments; C051367:complement C3bBa complex; D003179:Complement C3b; D011415:Complement Factor B