Sequence variability of simian immunodeficiency virus in a persistently infected rhesus monkey.
A juvenile rhesus monkey that was inoculated intravenously with molecularly cloned SIVmac239 became persistently infected. A modified polymerase chain reaction (PCR) procedure was used to specifically amplify full-length envelope (env) gene sequences from DNA extracted from peripheral blood mononuclear cells (PBMC), lymph node tissue, and cells infected with recovered virus at 69 and 93 weeks post-infection. Extensive sequence variability accumulated in vivo in spite of infection with molecularly cloned virus. In the central portion of env. sequence variability was largely confined to three discrete regions.
Burns
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New England Regional Primate Research Center, Harvard Medical School, Southborough, MA.
Desrosiers
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D015686:Gene Products, env / Q000235:genetics; D015752:Genes, env; D014644:Genetic Variation; D007963:Leukocytes, Mononuclear / Q000382:microbiology; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016097:Simian Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D015302:Simian Immunodeficiency Virus / Q000235:genetics*
D015686:Gene Products, env