Expression of P0- and P3-RNA from the normal and translocated c-myc allele in Burkitt's lymphoma cells.
We have studied the allele specific expression of c-myc P0- and P3-RNA in Burkitt's lymphoma (BL) cells. The steady state levels of P0-RNA show considerable variations in BL cells. Expression of P0-RNA was found to be restricted to the translocated allele, but could be induced by TPA from the normal allele. P0-transcription was particularly sensitive to inhibitors of protein synthesis compared to expression of P1-, P2- and P3-RNA. Transcription of P3-RNA is initiated in the first intron of the c-myc gene and has previously been described to be specific for translocated c-myc alleles in BL cells broken within exon 1 or intron 1. Here we show that P3-RNA is also expressed from an unrearranged c-myc gene. In the BL cell line Raji, substantial amounts of c-myc RNA are derived from the P3-promoter of the normal allele. This indicates that repression of the normal allele in BL cells does not include the P3-promoter. The potential coding capacity of P3-RNA is discussed.
Eick
D
D
Institut für Klinische Molekularbiologie und Tumorgenetik, Hämatologikum der GSF, München, Federal Republic of Germany.
Polack
A
A
Kofler
E
E
Lenoir
G M
GM
Rickinson
A B
AB
Bornkamm
G W
GW
D000483:Alleles; D000595:Amino Acid Sequence; D002051:Burkitt Lymphoma / Q000235:genetics*; D003513:Cycloheximide / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000235:genetics*; D016259:Genes, myc; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012334:RNA, Neoplasm / Q000032:analysis / Q000235:genetics*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014158:Transcription, Genetic; D014178:Translocation, Genetic
D012334:RNA, Neoplasm; D003513:Cycloheximide; D013755:Tetradecanoylphorbol Acetate