Structural and functional studies of the rat mitochondrial single strand DNA binding protein P16.
The rat mitochondrial single strand DNA binding protein (SSB) P16 was purified to apparent homogeneity by elution from single strand DNA agarose with ethidium bromide. Each monomer of P16 contains two tryptophan residues, and the intrinsic fluorescence from these residues is quenched upon binding to single strand polynucleotides. From fluorescence quench titrations of ligand to fixed amounts of DNA lattice, a binding site size of 8 or 9 nucleotides per P16 monomer was found. Measurement of the affinity of P16 for isolated sites by titration with either oligo(dT)8 or 5'-dephosphorylated oligo(dT)8 indicated values on the order of 10(7) M-1. P16 exhibited a binding preference for single strand DNA, poly(dT), and poly(dC) in comparison to double strand DNA, poly(U), or poly[d(A-T)]. Although it was not possible to show that P16 destabilizes double helical DNA or even poly[d(A-T)], binding of P16 does inhibit the process of renaturation as shown by inhibition of duplex formation between poly(dA) and poly(dT). The binding of saturating amounts of P16 to single strand poly(dT).oligo(dA)50 template-primers enhanced approximately 10-fold the activity of both the homologous mitochondrial DNA polymerase and the Escherichia coli DNA polymerase I Klenow fragment. However, the mitochondrial DNA primase was nearly completely inhibited by the saturation of the poly(dT) template with P16. Amino-terminal sequence analysis of P16 and a protease-insensitive, DNA binding domain (Mr approximately 6000) revealed that the DNA binding domain residues, at least in part, in the amino-terminal third of the P16 molecule. Furthermore, the amino-terminal sequence was found to be strikingly similar to that of the Xenopus laevis mtSSB-1 and to a lesser extent similar to E. coli SSB and E. coli F sex factor SSB.
Hoke
G D
GD
Department of Biochemistry and Molecular Biophysics, Virginia Commonwealth University, Richmond 23298.
Pavco
P A
PA
Ledwith
B J
BJ
Van Tuyle
G C
GC
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002458:Cell Fractionation; D003090:Coliphages / Q000378:metabolism; D004256:DNA Polymerase I / Q000378:metabolism; D004258:DNA Polymerase III / Q000302:isolation & purification / Q000378:metabolism; D019915:DNA Primase; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D007700:Kinetics; D008930:Mitochondria, Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D011089:Polydeoxyribonucleotides / Q000378:metabolism*; D012316:RNA Nucleotidyltransferases / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013379:Substrate Specificity
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D011089:Polydeoxyribonucleotides; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases; D004256:DNA Polymerase I; D004258:DNA Polymerase III