[Use of filter paper for blood specimen collection in poultry for use in the detection of antibodies to Newcastle disease virus].
Trials were conducted to verify the possibility of poultry blood sampling with filter papers for subsequent examination of the eluates for the presence (and level) of haemagglutination-inhibition antibodies against the Newcastle disease virus (NDV). Qualitative examination was performed in 294 paired samples of sera and eluates, representing 10 selective sets, coming from three vaccinated poultry flocks. The numbers of positive sera (dilution ratios 1:20 and higher) and positive eluates of filter papers (dilution ratios of 1: 2 and higher) were compared and it was found that there were 238 positive paired samples (81%) and 30 were negative (10.2%), hence, there were like reactions in 268 paired samples (91.2% of the total number of samples examined). It was only in 25 paired samples that positivity was recorded just in the sera: 22 times with a titre of 1:20 and three times with a titre of 1:40. In one case, positivity was recorded just in the eluate. The final titres were compared in 181 paired samples of sera and eluates, all diluted at a ratio of 1:2, and it was found that the concentration of the haemagglutination-inhibition antibodies in the eluates corresponded to serum dilution ratio of 1:20. Under this assumption, the antibodies were found to have the same titre in 164 paired samples (55.8%) during the quantitative evaluation. A lower titre was recorded in 82 eluate samples (27.9%) and a higher antibody titre in 48 eluate samples (16.3%) (in comparison with the antibody titres in the respective sera). The over-all average geometrical titre (GMT) of antibodies was 1:65 in the eluates and 1:75 in the sera.(ABSTRACT TRUNCATED AT 250 WORDS)
Jurajda
V
V
Vysoká skola veterinárni, Brno.
Schmidtová
B
B
Rybová
L
L
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D001800:Blood Specimen Collection / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D002645:Chickens / Q000382:microbiology*; D006385:Hemagglutination Inhibition Tests; D009522:Newcastle disease virus / Q000276:immunology*
D000914:Antibodies, Viral
Pouzití filtraccního papíru k odbĕru krve u drůbeze a k následnému vysetrení prítomnosti protilátek proti viru Newcastleské nemoci.