Immunochemical detection of human blood in feces.
We have developed a new immunochemical test for fecal occult blood utilizing enzyme-linked immunosorbent assay (ELISA) of human hemoglobin (HbAo) and transferrin (Tf) simultaneously. The ELISA had a sensitivity of about 15 ng/ml Hb, and the measurable range was 1.5-750 micrograms Hb per g feces. The stability of Tf in feces was greater than that of Hb. In 17 out of 18 patients with colon cancer, 8 out of 15 patients with colon polyps, and 11 out of 20 patients with upper-gastrointestinal disorders. The Hb and Tf values were more than 10 micrograms/g feces, in terms of Hb concentration. The ELISA for human fecal HbAo and Tf might be useful for the diagnosis of gastrointestinal disorders.
Uchida
K
K
Kyoto Medical Science Laboratory Incorporation, Japan.
Matsuse
R
R
Miyachi
N
N
Okuda
S
S
Tomita
S
S
Miyoshi
H
H
Hirata
I
I
Tsumoto
S
S
Ohshiba
S
S
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000502:physiology; D005243:Feces / Q000737:chemistry; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D009780:Occult Blood; D010949:Plasma; D014168:Transferrin / Q000032:analysis
D000911:Antibodies, Monoclonal; D006454:Hemoglobins; D014168:Transferrin