Capillary electrophoresis in 2 and 5 microns diameter capillaries: application to cytoplasmic analysis.
Capillary electrophoresis with electrochemical detection in 2- and 5-micron capillaries has been developed to study ultrasmall biological environments. Sample volumes as low as 270 fL have been injected into the electrophoresis capillary with subattomole detection limits for easily oxidized species. We have applied this method to the analysis of single cell cytoplasm. Sampling of the cytoplasm is accomplished by inserting one end of the electrophoresis capillary directly into a single nerve cell. The high-voltage end of the electrophoresis capillary has been etched with hydrofluoric acid to form a microinjector. This injection scheme represents an improvement over those previously used for similar applications. The excellent selectivity of this method is demonstrated for catecholamine and indolamine neurotransmitters and their metabolites found in the invertebrate system, the pond snail Planorbis corneus.
Olefirowicz
T M
TM
Department of Chemistry, Penn State University, University Park 16802.
Ewing
A G
AG
D000818:Animals; D003593:Cytoplasm / Q000737:chemistry*; D004586:Electrophoresis / Q000379:methods*; D008832:Microchemistry; D008845:Microinjections / Q000295:instrumentation; D009474:Neurons / Q000737:chemistry*; D012908:Snails