Identification and sequence analysis of the maize stripe virus major noncapsid protein gene.
The maize stripe virus (MStV) major noncapsid protein (NCP) gene was characterized, and the location of the NCP gene was identified among the 5-RNA, 18-kb genome. A 12-amino-acid sequence of the NCP was compared with nucleotide sequence data for MStV RNAs 3 and 4 and was found to align perfectly within a 528-nucleotide open reading frame (ORF) of RNA 4. The amino acid composition of purified NCP was almost identical to that deduced from the putative coding region. The deduced NCP molecular weight was 19,815, very similar to that determined by SDS-PAGE analysis of the purified NCP. In vitro transcription and translation analysis of the cDNA representing this region showed unequivocally that this region encoded the NCP. Primer extension analysis using a synthetic oligonucleotide complementary to a sequence near the 5' end of the coding region revealed that the NCP ORF is located 61 nucleotides from the 5' end of RNA 4.
Huiet
L
L
Department of Plant Pathology, University of California, Davis 95616.
Klaassen
V
V
Tsai
J H
JH
Falk
B W
BW
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009841:Oligonucleotides; D010940:Plant Proteins / Q000235:genetics*; D010942:Plant Viruses / Q000235:genetics*; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000235:genetics; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics; D003313:Zea mays / Q000382:microbiology*
D000596:Amino Acids; C066396:NCP protein, Maize stripe virus; D009841:Oligonucleotides; D010940:Plant Proteins; D012367:RNA, Viral; D014764:Viral Proteins; D015678:Viral Structural Proteins