Conjugation of chemical carcinogens by primary cultures of human hepatocytes.
1. The conjugation of benzo[a]pyrene (BP) and 2-acetylaminofluorene (AAF) was investigated in primary cultures of human hepatocytes. Human hepatocytes conjugated 12.5-63% of the BP and 1.7-52% of the AAF to sulphates and glucuronides over a thousand-fold concentration range. 2. BP is conjugated to glucuronides from non-detectable levels to 50%, and to sulphates from non-detectable levels to 30%. The major conjugated metabolites are the highly polar metabolites. 3. AAF is conjugated to glucuronides from 1.5 to 51% and to sulphates from 0.2 to 12%. The C-hydroxylated AAF metabolites were conjugated to glucuronides more than N-hydroxy AAF and aminofluorene metabolites.
Monteith
D K
DK
Department of Biological Sciences, Indiana-Purdue University, Fort Wayne 46805.
Michalopoulos
G
G
Strom
S C
SC
D015073:2-Acetylaminofluorene / Q000378:metabolism*; D001564:Benzo(a)pyrene / Q000378:metabolism*; D002273:Carcinogens / Q000378:metabolism*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D005965:Glucuronates / Q000378:metabolism; D006801:Humans; D006900:Hydroxylation; D008658:Inactivation, Metabolic; D008099:Liver / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism
D002273:Carcinogens; D005965:Glucuronates; D013431:Sulfates; D001564:Benzo(a)pyrene; D015073:2-Acetylaminofluorene