Ki-67 immunostaining in uveal melanoma. The effect of pre-enucleation radiotherapy.
The reactivity of 33 choroidal and ciliary body melanomas with monoclonal antibody Ki-67, which recognizes a proliferation associated nuclear antigen, has been assessed and compared with clinicopathologic parameters. In 23 cases, 8 Gy irradiation was given 2 days before enucleation. Nonirradiated melanomas had a significantly higher proliferation rate as defined by staining with monoclonal antibody Ki-67 as compared with irradiated tumors (P = 0.007). Similarly, a strong relationship was found between pre-enucleation irradiation and low mitotic activity (P = 0.001). There was no significant correlation between the presence of Ki-67-positive nuclei and histologic classification, largest tumor diameter, localization of the tumor, age, sex, scleral invasion, pigmentation, and lymphocytic infiltration. The relevance of Ki-67 immunohistochemistry for the assessment of the life prognosis of patients with uveal melanoma has to be studied prospectively.
Mooy
C M
CM
Department of Ophthalmology, Erasmus University, Rotterdam, The Netherlands.
de Jong
P T
PT
Van der Kwast
T H
TH
Mulder
P G
PG
Jager
M J
MJ
Ruiter
D J
DJ
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000912:Antibodies, Neoplasm / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology; D002455:Cell Division / Q000528:radiation effects; D002830:Choroid Neoplasms / Q000276:immunology / Q000473:pathology* / Q000532:radiotherapy; D002924:Ciliary Body / Q000276:immunology / Q000473:pathology*; D015353:Eye Enucleation; D006801:Humans; D008545:Melanoma / Q000276:immunology / Q000473:pathology* / Q000532:radiotherapy; D014604:Uveal Neoplasms / Q000276:immunology / Q000473:pathology* / Q000532:radiotherapy
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm