title: In vitro fertilization of horse follicular oocytes matured in vitro.
abstract: In vitro fertilizing ability of stallion spermatozoa was assessed using horse follicular oocytes matured in vitro. After collection, stallion spermatozoa were either: 1) washed and incubated in TALP medium with 3 mg/ml bovine serum albumin (BSA) and 10 micrograms/ml heparin for 4h, 2) washed and incubated in TALP with 3 mg/ml BSA for 3 h and cultured for a further 1 h with 1 mM caffeine and 5 mM dbcAMP, 3) washed and incubated in TALP medium with 3 mg/ml BSA at pH 7.9-8.2 for 2-4 h, or 4) diluted and incubated in TALP medium with 10 mg/ml BSA and 7.14 microM calcium ionophore A 23187 for 5-10 min followed by washing. After a given pretreatment, suspensions were diluted into B2 medium to a concentration of 5 x 10(6) sperm/ml and co-incubated with oocytes for 12 h or 24-48 h. In the ionophore-treated group, 18 of 54 oocytes (33%) were fertilized by 12 h, and 11 of 45 (24%) cleaved by 24-48 h. Evidence of fertilization was not found in the oocytes incubated with spermatozoa from other treatment procedures.
authors.affiliation: Thoroughbred Breeders' Association, Equine Fertility Unit, Newmarket, Suffolk, United Kingdom.
authors.forename: J J
authors.initials: JJ
authors.lastname: Zhang
authors.forename: L Z
authors.initials: LZ
authors.lastname: Muzs
authors.forename: M S
authors.initials: MS
authors.lastname: Boyle
mesh_terms: D000818:Animals; D005260:Female; D005307:Fertilization in Vitro; D006736:Horses; D008297:Male; D009865:Oocytes / Q000502:physiology*; D009866:Oogenesis; D006080:Ovarian Follicle / Q000502:physiology; D013075:Sperm Capacitation; D013081:Sperm Motility