title: Rapid estimation of serum myoglobin concentration during rhabdomyolysis with a latex-agglutination test (Rapi-Tex).
abstract: A latex-agglutination test (Rapi-Tex) was used for semiquantitative estimation of myoglobin concentration in 89 serum samples obtained from ten patients undergoing lower-limb arterial embolectomy. Each serum sample was tested with Rapi-Tex both undiluted and after 1:50 dilution with saline. The myoglobin concentration was additionally measured with enzyme-immunoassay. In the undiluted samples there were five 'false negative' Rapi-Tex results--two in the myoglobin concentration interval 0-300 micrograms and three in the samples with myoglobin exceeding 25,000 micrograms/l--and four 'false positive' results. None of the diluted samples gave rise to false classification. Using 1:50 diluted serum, the negative predictive value of the Rapi-Tex test for identification of a myoglobin concentration greater than 5,000 micrograms/l was 0.99 (95% confidence limits 0.94-1.00), and the positive predictive value was 1.00 (0.61-1.00). Rapi-tex is concluded to be a useful emergency test for severe hypermyoglobinemia.
authors.affiliation: Department of Thoracic and Vascular Surgery, Aalborg Hospital, Denmark.
authors.forename: T
authors.initials: T
authors.lastname: Andersen
authors.forename: P J
authors.initials: PJ
authors.lastname: Jørgensen
authors.forename: E
authors.initials: E
authors.lastname: Toft
authors.forename: L K
authors.initials: LK
authors.lastname: Nielsen
authors.forename: K N
authors.initials: KN
authors.lastname: Hansen
mesh_terms: D058186:Acute Kidney Injury / Q000097:blood / Q000175:diagnosis / Q000209:etiology; D000368:Aged; D000369:Aged, 80 and over; D000372:Agglutination Tests / Q000379:methods*; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009211:Myoglobin / Q000097:blood*; D012206:Rhabdomyolysis / Q000097:blood* / Q000150:complications
chemical_list: D009211:Myoglobin