title: High-performance liquid chromatography patterns of mycolic acids as criteria for identification of Mycobacterium chelonae, Mycobacterium fortuitum, and Mycobacterium smegmatis.
abstract: Rapidly growing mycobacteria of clinical significance were identified by mycolic acids detected with high-performance liquid chromatography. Mycolic acids from whole cells were extracted, derivatized, and detected by a modified high-performance liquid chromatography procedure in less than 3 h. Use of an internal standard allowed differentiation of Mycobacterium chelonae and Mycobacterium fortuitum by comparison of relative retention times. Peak height ratios were used for subidentification of M. chelonae strains; however, M. fortuitum and Mycobacterium smegmatis could not be separated by this system.
authors.affiliation: Division of Bacterial Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
authors.forename: W R
authors.initials: WR
authors.lastname: Butler
authors.forename: J O
authors.initials: JO
authors.lastname: Kilburn
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D006801:Humans; D009161:Mycobacterium / Q000032:analysis* / Q000145:classification / Q000302:isolation & purification; D009171:Mycolic Acids / Q000302:isolation & purification* / Q000592:standards; D009170:Nontuberculous Mycobacteria / Q000032:analysis / Q000145:classification / Q000302:isolation & purification; D012015:Reference Standards; D013045:Species Specificity; D013696:Temperature
chemical_list: D003470:Culture Media; D009171:Mycolic Acids