[Isolation of low molecular weight cytoplasmic regulators from the rat liver inducing the electrophoretic transport of K+ ions and phosphate across the inner mitochondrial membrane].
A water-soluble thermostable factor from rat liver cytoplasm whose activity decreases during starvation, causes the uncoupling of oxidative phosphorylation and stimulates pyruvate oxidation in rat liver mitochondria. The activity of this factor is insensitive to pronase treatment. Gel filtration and ion-exchange chromatography resulted in three low molecular weight water-soluble fractions which bear a negative charge at alkaline values of pH and induce electrophoretic transport of K+ and phosphate across the inner mitochondrial membrane. The effect of this factor on K+ transport is manifested at pH less than or equal to 7.0, that on phosphate transport-at pH 6.5-7.6.
Gaĭnutdinov
M Kh
MKh
Zakharova
T N
TN
Ishmukhamedov
R N
RN
Konov
V V
VV
Safarov
K S
KS
D000818:Animals; D001692:Biological Transport; D002850:Chromatography, Gel; D003593:Cytoplasm / Q000737:chemistry* / Q000502:physiology; D007425:Intracellular Membranes / Q000378:metabolism*; D008099:Liver / Q000737:chemistry* / Q000502:physiology; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D008970:Molecular Weight; D010710:Phosphates / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D051381:Rats
D010710:Phosphates; D011188:Potassium
Vydelenie nizkomolekuliarnykh tsitoplazmaticheskikh reguliatorov iz pecheni krysy, indutsiruiushchikh ĺektroforeticheskiĭ transport ionov K+ i fosfata cherez vnutrenniuiu membranu mitokhondriĭ.