Identification of a novel platelet-derived neutrophil-chemotactic polypeptide with structural homology to platelet-factor 4.
A novel protein, NAP-4, could be isolated from human platelet lysates. NAP-4 preparations induced chemotaxis of human neutrophils with an ED50 near 400 ng/ml. Purification by anti NAP-1/IL-8 affinity chromatography and reversed phase HPLC revealed a single peak showing a single line upon SDS-PAGE corresponding to a Mr of 8000. NH2-terminal sequence analysis indicated an unique sequence showing strong homology to human platelet factor 4 and weak homology to tumor necrosis factor alpha as well. The most interesting finding is the absence of the first two cysteins, known to be strongly conserved in members of the family of platelet-factor 4-like host defense cytokines.
Schröder
J M
JM
Department of Dermatology, University of Kiel, Federal Republic of Germany.
Sticherling
M
M
Persoon
N L
NL
Christophers
E
E
D000595:Amino Acid Sequence; D001792:Blood Platelets / Q000502:physiology*; D002630:Chemotactic Factors / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D010978:Platelet Factor 4 / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D002630:Chemotactic Factors; D014409:Tumor Necrosis Factor-alpha; C066258:platelet-derived neutrophil chemotactic peptide; D010978:Platelet Factor 4