Preliminary crystal structure analysis of an Fab specific for a Salmonella O-polysaccharide antigen.
The Fab from an IgG1, lambda murine monoclonal antibody with specificity for the O-polysaccharide antigen of Salmonella typhimurium has been crystallized in the absence and presence of hapten. The conditions for crystal growth were vapor diffusion equilibration with 16 to 23% polyethylene glycol 8000 solutions. Data have been collected from crystals of the complex in space group P212121, a = 60.6 A, b = 111.3 A, c = 61.1 A, and refinement of a molecular replacement solution is underway.
Rose
D R
DR
Division of Biological Sciences, National Research Council, Ottawa, Ontario, Canada.
Cygler
M
M
To
R J
RJ
Przybylska
M
M
Sinnott
B
B
Bundle
D R
DR
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D008969:Molecular Sequence Data; D019081:O Antigens; D011105:Polyisoprenyl Phosphate Sugars / Q000737:chemistry* / Q000276:immunology; D012486:Salmonella typhimurium / Q000276:immunology*; D014961:X-Ray Diffraction
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D019081:O Antigens; C062656:O-specific polysaccharide, Salmonella; D011105:Polyisoprenyl Phosphate Sugars