Isolation of cyclo(His-Pro)-like immunoreactivity from human urine and demonstration of its immunologic, pharmacologic, and physico-chemical identity with the synthetic peptide.
Measurements of cyclo(His-Pro) levels in human urine were carried out by specific radioimmunoassay. Cyclo(His-Pro)-like immunoreactivity in Human urine was found to be immunologically, pharmacologically, and physico-chemically identical to that of synthetic cyclo(His-Pro). The concentration of urinary cyclo(His-Pro) in 24-h collection was 1133.8 +/- 122.5 nmol/L, with a range of 606 to 1865 nmol/L. The daily excretion rate of cyclo(His-Pro) was 1812 +/- 248 nmol cyclo(His-Pro)/g creatinine, or 1814 +/- 199 nmol cyclo(His-Pro/day.
Prasad
C
C
Laboratory of Neurosciences, Pennington Biomedical Research Center, Baton Rouge, LA.
Ragan
F A
FA
Hilton
C W
CW
D000328:Adult; D001667:Binding, Competitive; D002606:Charcoal; D002648:Child; D002845:Chromatography; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D010456:Peptides, Cyclic / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology / Q000652:urine*; D010879:Piperazines / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology / Q000652:urine*; D011863:Radioimmunoassay
D010456:Peptides, Cyclic; D010879:Piperazines; D002606:Charcoal; C015924:histidyl-proline diketopiperazine