Cation-dependent solubilization of rat thymocyte chromatin is closely related to decondensation of the nuclei.
The cation-dependent solubilization of rat thymocyte chromatin has been compared with decondensation of the nuclei as a function of sodium phosphate-mediated changes in the concentration of Mg2+ and Na+. After digestion of the nuclei with DNase I or Micrococcus nuclease for a time just sufficient to permit extraction of a maximal amount of chromatin (minimum digestion), solubilization of most of the chromatin was found to occur with the same cation dependency as decondensation of untreated nuclei, while further digestion changed the ionic requirements for solubilization. The cation-dependency of the chromatin solubility and of the nuclear decondensation also exhibited the same variations with temperature. The chromatin in the nuclei became up to 4-times more sensitive to DNase I by decondensation, which also induced a shift in the DNase I cleavage mode from a 200 bp to a 100 bp repeat pattern. In contrast, the sensitivity to Micrococcus nuclease appeared to be nearly unchanged. These results suggest that solubilization of chromatin prepared by a mild endonuclease treatment occurs as a direct consequence of structural changes in the chromatin which take place during decondensation of the nuclei.
Engelhardt
M
M
Biochemical Department B, Panum Institute, University of Copenhagen, Denmark.
D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism*; D002843:Chromatin / Q000737:chemistry*; D003850:Deoxyribonuclease I / Q000378:metabolism; D005260:Female; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology*; D010710:Phosphates / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000494:pharmacology*; D012995:Solubility; D013601:T-Lymphocytes / Q000737:chemistry* / Q000648:ultrastructure
D002843:Chromatin; D010710:Phosphates; D012964:Sodium; D003850:Deoxyribonuclease I; D008274:Magnesium; C018279:sodium phosphate