Cytotropism of Theiler's murine encephalomyelitis viruses in oligodendrocyte-enriched cultures.
The cytotropism of two strains, GDVII and DA, of Theiler's murine encephalomyelitis viruses (TMEV) was studied in the oligodendrocyte-enriched murine neural cell cultures. Both GDVII and DA caused cytopathic effects in the neural cell cultures, and double immunostaining for galactocerebroside (Gal-Cer), a marker molecule for oligodendrocyte, and viral antigens disclosed a dual expression of Gal-Cer and viral antigens in over 80% of cells in both cultures 24 h after infection with either GDVII or DA. The kinetics of cell-free and cell-associated infectivity were not significantly different between two cultures. These in vitro observations suggest that neither replication in oligodendrocyte nor cell-associated infectivity is a sole factor in discriminating those two subgroups of TMEV with regard to the demyelinating activity, and that virus cell binding may play an important role in virus persistence and TMEV-induced demyelination.
Ohara
Y
Y
Department of Neurological Sciences, Tohoku University School of Medicine, Sendai, Japan.
Konno
H
H
Iwasaki
Y
Y
Yamamoto
T
T
Terunuma
H
H
Suzuki
H
H
D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis; D002465:Cell Movement; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D003711:Demyelinating Diseases; D005260:Female; D005699:Galactosylceramides / Q000096:biosynthesis; D007700:Kinetics; D004680:Maus Elberfeld virus / Q000502:physiology*; D051379:Mice; D008809:Mice, Inbred C3H; D009474:Neurons / Q000382:microbiology* / Q000473:pathology; D009836:Oligodendroglia / Q000382:microbiology / Q000473:pathology; D013045:Species Specificity; D014779:Virus Replication
D000956:Antigens, Viral; D005699:Galactosylceramides; C002454:galactocerebroside