Synthesis and characterization of oligodeoxynucleotides containing the mutagenic base analogue 4-O-ethylthymine.
A method for the preparation of oligonucleotides containing the mutagenic base 4-O-ethylthymine is described for the first time. Use of p-nitrophenylethyl type base protecting groups together with phosphitetriester solid-phase methodology makes possible the rapid and efficient preparation of oligonucleotides bearing 4-O-ethylthymine, while standard base protecting groups are not compatible with the presence of this base. Possible applications of this methodology are discussed.
Fernandez-Forner
D
D
Department of Organic Chemistry, University of Barcelona, Spain.
Palom
Y
Y
Ikuta
S
S
Pedroso
E
E
Eritja
R
R
D001483:Base Sequence; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009154:Mutation; D009838:Oligodeoxyribonucleotides / Q000032:analysis / Q000138:chemical synthesis* / Q000378:metabolism; D013941:Thymine / Q000031:analogs & derivatives* / Q000032:analysis
D009838:Oligodeoxyribonucleotides; C040384:4-ethylthymine; D013941:Thymine