The omnipotent suppressor SUP45 affects nucleic acid metabolism and mitochondrial structure.
Yeast (Saccharomyces cerevisiae) strains sensitive to a variety of drugs were used to select for novobiocin-resistant mutants that were simultaneously temperature-sensitive. The mutants remained as sensitive as the parent strains to a wide range of drugs other than novobiocin, and did not exhibit any suppression of suppressible auxotrophic markers. At the non-permissive temperature, the mutant cells arrested mainly as unbudded cells, and were instantly defective in DNA and RNA synthesis, but not protein synthesis. The cloned wild-type gene was identified as SUP45, which has been previously implicated in the translation process. Our results suggest that SUP45 may have a function in addition to, or different from, the one that has been assigned to it previously.
Pocklington
M J
MJ
Department of Genetics, University of Leicester, U.K.
Johnston
L
L
Jenkins
J R
JR
Orr
E
E
D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000096:biosynthesis* / Q000187:drug effects; D004272:DNA, Mitochondrial / Q000096:biosynthesis; D004352:Drug Resistance, Microbial; D005656:Fungal Proteins / Q000096:biosynthesis / Q000187:drug effects; D005800:Genes, Fungal; D016153:Genes, Suppressor / Q000502:physiology*; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000648:ultrastructure*; D009154:Mutation; D009675:Novobiocin / Q000494:pharmacology; D010641:Phenotype; D012331:RNA, Fungal / Q000096:biosynthesis* / Q000187:drug effects; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D005656:Fungal Proteins; D012331:RNA, Fungal; D009675:Novobiocin