title: Regulation of LH/hCG receptor by gonadotropins in rat ovary.
abstract: To investigate the regulation of the LH/hCG receptor gene by gonadotropins, we examined the effect of PMSG and hCG on the expression of LH/hCG receptor in immature rat ovary. Northern blot analysis of ovarian RNA revealed a major mRNA of 5400 nucleotides and minor species of 7500, 3600, 2300, and 1200 nucleotides, and PMSG treatment slightly increased the intensity of all LH/hCG receptor messengers. Subsequently, hCG treatment decreased the number of LH/hCG receptor by day 2 and mRNA levels by 12h after injection. The level of mRNA recovered and increased 5-fold of control by day 6, then returned to control levels by day 10, followed by slower decline in LH/hCG receptor in plasma membrane. These studies demonstrate that the effects of PMSG and hCG on the number of LH/hCG receptor are closely related to the actions of these hormones on LH/hCG receptor messenger levels.
authors.affiliation: Department of Obstetrics and Gynecology, Gunma University, School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Minegishi
authors.forename: Y
authors.initials: Y
authors.lastname: Takakura
authors.forename: K
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Hasegawa
authors.forename: Y
authors.initials: Y
authors.lastname: Ibuki
authors.forename: M
authors.initials: M
authors.lastname: Igarashi
mesh_terms: D000375:Aging; D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D010053:Ovary / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D011974:Receptors, LH / Q000187:drug effects / Q000235:genetics*; D012741:Sexual Maturation
chemical_list: D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine; D012333:RNA, Messenger; D011974:Receptors, LH