title: Chromosomal localization of the human alpha-L-iduronidase gene (IDUA) to 4p16.3.
abstract: The lysosomal hydrolase alpha-L-iduronidase (IDUA) is one of the enzymes in the metabolic pathway responsible for the degradation of the glycosaminoglycans heparan sulfate and dermatan sulfate. In humans a deficiency of IDUA leads to the accumulation of glycosaminoglycans, resulting in the lysosomal storage disorder mucopolysaccharidosis type I. A genomic subclone and a cDNA clone encoding human IDUA were used to localize IDUA to chromosome 4p16.3 by in situ hybridization and this was confirmed by Southern blot analysis. This localization is different from that of a previous report mapping IDUA to chromosome 22 and places the gene for IDUA in the same region of chromosome 4 as the Huntington disease gene. Measurement of expressed human IDUA activity in human-mouse hybrid cell lines confirmed that IDUA is on chromosome 4.
authors.affiliation: Department of Chemical Pathology, Adelaide Children's Hospital, Australia.
authors.forename: H S
authors.initials: HS
authors.lastname: Scott
authors.forename: L J
authors.initials: LJ
authors.lastname: Ashton
authors.forename: H J
authors.initials: HJ
authors.lastname: Eyre
authors.forename: E
authors.initials: E
authors.lastname: Baker
authors.forename: D A
authors.initials: DA
authors.lastname: Brooks
authors.forename: D F
authors.initials: DF
authors.lastname: Callen
authors.forename: G R
authors.initials: GR
authors.lastname: Sutherland
authors.forename: C P
authors.initials: CP
authors.lastname: Morris
authors.forename: J J
authors.initials: JJ
authors.lastname: Hopwood
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22; D002894:Chromosomes, Human, Pair 4; D015342:DNA Probes; D006801:Humans; D006822:Hybrid Cells / Q000378:metabolism; D007068:Iduronidase / Q000235:genetics* / Q000276:immunology; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal; D015342:DNA Probes; D007068:Iduronidase