title: Particulate (high-molecular-mass) and soluble alkaline phosphatase in urine determined by high-performance liquid chromatography.
abstract: We detected in urine by HPLC two enzyme fractions of alkaline phosphatase (AP, EC 3.1.3.1), soluble and particulate, analogous to those in serum. The second fraction was eluted with high-salt-content eluent at the same elution time as high-molecular-mass, or particulate, AP in serum. AP characterization in urine from a patient with acute rejection crisis showed a greater sensitivity of the particulate form to treatment with L-phenylalanine, which suggests a higher content of intestinal-type AP in the particulate form. The soluble fraction showed a more liver-type AP behavior. Changes in the chromatograms after the sample was treated with 1-butanol and Triton X-100 support a membrane origin of the particulate AP. Urinalyses from patients with acute renal disease showed increased activity of soluble and particulate AP, with a relatively greater increase of particulate AP.
authors.affiliation: Klinik für Kinder und Jugendliche der Friedrich-Alexander Universität, Erlangen-Nürnberg, F.R.G.
authors.forename: E
authors.initials: E
authors.lastname: Schoenau
authors.forename: K H
authors.initials: KH
authors.lastname: Herzog
authors.forename: D
authors.initials: D
authors.lastname: Michalk
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000097:blood / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007674:Kidney Diseases / Q000201:enzymology; D012995:Solubility
chemical_list: D000469:Alkaline Phosphatase