Effect of supplementing cardioplegic solution with deferoxamine on reperfused human myocardium.
Fourteen randomized patients undergoing myocardial revascularization were divided into group A standard hypothermic cardioplegic solution) and group B (the same cardioplegic solution supplemented with deferoxamine 1000 mg/L). In all patients myocardial biopsy specimens were obtained before ischemia and during reperfusion and were assessed for chemiluminescence (to indirectly determine oxygen-free radical activity) and for electron microscopic studies. Chemiluminescence in group A showed a photoemission of 36.5 +/- 1.5 cpm/mg protein X10(-3) for the preischemia samples and 72 +/- 5.7 cpm/mg protein X10(-3) for the reperfusion samples (p less than 0.01). In the patients who received deferoxime (group B), values for chemiluminescence for preischemia and reperfusion samples were not significantly different. Electron microscopic studies showed a significant increase in grade 4 (severely damaged) mitochondria in reperfusion biopsy specimens from both groups as compared with preischemia samples. However, reperfusion samples from group B showed a better preservation of myocardial cells with marked reduction of grade 4 (severely damaged) mitochondria. These results support the hypothesis that oxygen-free radicals are responsible in part for the production of reperfusion injury in the human heart. They suggest that this mechanism may be at least partially controlled by adding an iron chelating agent such as deferoxime.
Ferreira
R
R
Department of Cardiovascular Service (ECAVI), Policlinico Bancario, Buenos Aires, Argentina.
Burgos
M
M
Milei
J
J
Llesuy
S
S
Molteni
L
L
Hourquebie
H
H
Boveris
A
A
D002314:Cardioplegic Solutions; D001026:Coronary Artery Bypass; D003676:Deferoxamine / Q000008:administration & dosage*; D006321:Heart / Q000187:drug effects*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008163:Luminescent Measurements; D008875:Middle Aged; D008929:Mitochondria, Heart / Q000648:ultrastructure; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure; D013318:Stroke Volume
D002314:Cardioplegic Solutions; D006861:Hydrogen Peroxide; D003676:Deferoxamine