Phenotypic suppression by aminoglycoside antibiotics of mutations blocking erythromycin biosynthesis in Saccharopolyspora erythraea.
The presence of a sublethal amount of apramycin, the aminoglycoside antibiotic used as a selectable marker in transformations of Saccharopolyspora erythraea with pKC505 and related plasmids, was found to suppress phenotypically the S. erythraea eryB25 and eryB26 mutations blocking erythromycin biosynthesis in this organism, probably by the effect of mistranslation.
Lewandowska-Skarbek
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M
School of Pharmacy, University of Wisconsin, Madison 53706.
Hutchinson
C R
CR
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004917:Erythromycin / Q000096:biosynthesis*; D006094:Gram-Positive Bacteria / Q000187:drug effects / Q000235:genetics*; D009154:Mutation; D009328:Nebramycin / Q000031:analogs & derivatives* / Q000494:pharmacology; D010641:Phenotype; D013489:Suppression, Genetic / Q000187:drug effects*; D014169:Transformation, Bacterial
D000900:Anti-Bacterial Agents; D009328:Nebramycin; C011666:apramycin; D004917:Erythromycin