ELISA in detection of Entamoeba histolytica antigens in stools.
The modified double antibody sandwich ELISA was applied to detect E. histolytica antigens in the stool samples of 20 patients have amoebiasis (symptomatic or asymptomatic), 10 with intestinal parasites other than E. histolytica and 10 healthy individuals. The method was found to be sensitive (90%) and specific (85%). It is concluded that ELISA could be applied for a large scale epidemiological study and immunodiagnosis of amoebiasis.
Kabil
S M
SM
Department of Tropical Medicine, Benha, Egypt.
Hassan
M M
MM
Atta
M
M
el Ghysha
O
O
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D004404:Dysentery, Amebic / Q000175:diagnosis*; D004748:Entamoeba histolytica / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000737:chemistry*; D006801:Humans; D011237:Predictive Value of Tests
D000953:Antigens, Protozoan