An immunoassay for the quantitation of cell membrane MHC class II antigen.
A method is described for the quantitative assay of cellular MHC class II proteins. The assay has been developed for special use with epithelial cells isolated from the intestine, but has been successfully used with other cell types. It comprises a competitive immunoassay of anti-class II activity in cell lysates, using IgG-coated Sephacryl S300 as the solid phase and is sensitive over the range 1 ng-1 microgram.
Whiting
C V
CV
Department of Veterinary Medicine, University of Bristol, Langford, U.K.
Bland
P W
PW
D000180:Acrylic Resins; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001667:Binding, Competitive; D002462:Cell Membrane / Q000276:immunology; D004848:Epithelium / Q000276:immunology; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D007074:Immunoglobulin G / Q000276:immunology; D007202:Indicators and Reagents; D007422:Intestines / Q000276:immunology; D008297:Male; D051379:Mice; D011863:Radioimmunoassay / Q000379:methods*; D051381:Rats; D015203:Reproducibility of Results
D000180:Acrylic Resins; D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D007074:Immunoglobulin G; D007202:Indicators and Reagents; C037239:Sephacryl Superfine