[Localization of the structural gene for botulinum neurotoxin type A using synthetic DNA probe for neurotoxin light chain].
To obtain the information on the genetic control of toxin production in the botulism causative agents, the oligonucleotides were synthesized as the molecular probes by translation of the amino acid sequence of the botulinic type A neurotoxin. The optimal conditions for hybridization of botulinic DNA with the synthetic DNA probes were determined and the probes specificity was demonstrated. The DNA fragments homologous to the probes used were shown to belong to bacterial genome, but not to bacteriophage one.
Stepanova
M V
MV
Bystrov
N S
NS
Severtsova
N V
NV
Korobko
V G
VG
Bulatova
T I
TI
Zubova
T M
TM
Evstigneev
V I
VI
Smirnov
E V
EV
Vertiev
Iu V
IuV
D000595:Amino Acid Sequence; D001483:Base Sequence; D001905:Botulinum Toxins / Q000235:genetics*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D004269:DNA, Bacterial; D009498:Neurotoxins; D001905:Botulinum Toxins
Poisk lokalizatsii strukturnogo gena botulinicheskogo neĭrotoksina tipa A s ispol'zovaniem sinteticheskogo DNK-zonda k legkoĭ tsepi neĭrotoksina.