Serological characterization of murine monoclonal antibodies directed against acquired B red cells.
Balb/c mice were immunized with acquired B red cells. Twelve clones specific for acquired B red cells were obtained from two fusions. A detailed investigation of three clones is reported here. These antibodies appear to be directed to the B-like epitope since they are inhibited by galactosamine and fail to react after acetylation of red cells. E 231 is an example of a series of antibodies closely specific for acquired B red cells which can be useful in elucidating some AB0 typing problems. E 167 and F 47 showed a cross-reactivity with A1 red cells and a synthetic A trisaccharide. No affinity for B antigen could be demonstrated for any of the antibodies.
Janvier
D
D
Centre de Secteur d'Hémobiologie-Transfusion, Hôpital St-Louis, Paris, France.
Veaux
S
S
Reviron
M
M
Guignier
F
F
Benbunan
M
M
D000017:ABO Blood-Group System / Q000276:immunology*; D000107:Acetylation; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001788:Blood Grouping and Crossmatching / Q000379:methods; D002241:Carbohydrates / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology*; D006384:Hemagglutination / Q000276:immunology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009439:Neuraminidase; D014312:Trisaccharides / Q000276:immunology
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D014312:Trisaccharides; D009439:Neuraminidase