Luminol enhanced chemiluminescence of the perfused rat heart during ischemia and reperfusion.
We show that the production of Luminol reactive oxygen radicals in the perfused rat heart under ischemia and reperfusion can be monitored continuously by measuring the chemiluminescence of Luminol-perfused hearts. Luminol did not affect the monitored physiological parameters of the hearts. Chemiluminescence increased during ischemia and reperfusion. Superoxide dismutase treatment of the heart before ischemia, but not catalase, abolished these increases.
Kumar
C
C
Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia 19104-6089.
Okuda
M
M
Ikai
I
I
Chance
B
B
D000818:Animals; D002021:Buffers; D002374:Catalase / Q000494:pharmacology; D003326:Coronary Circulation; D003327:Coronary Disease / Q000378:metabolism*; D003971:Diastole; D005609:Free Radicals; D006352:Heart Ventricles / Q000503:physiopathology; D049449:Luminescence; D008165:Luminol / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D015424:Reperfusion; D013482:Superoxide Dismutase / Q000494:pharmacology; D013599:Systole
D002021:Buffers; D005609:Free Radicals; D008165:Luminol; D002374:Catalase; D013482:Superoxide Dismutase; D010100:Oxygen