Immunohistochemical study of intestinal lamina propria cells in the rat, with special reference to Ia antigen expression.
A previous study by Osogoe and Yanagi (1987a) has demonstrated that a large proportion of the lamina propria cells (LPC), mostly reticulum cells, in the core of the rat jejunal villi exhibit a strong labeling with [14C]adenine [( 14C]A). To obtain further information on such LPC, immunohistochemical detection of either Ia antigen or IgA on intestinal LPC was carried out in adult rats. It was found that almost all the LPC in the villous core of the small intestine express Ia antigen, and that in this area IgA-containing B cells also occur in unexpectedly large numbers. However, the number of Ia-positive non-B cells was greater than that of IgA-containing B cells which are also Ia-positive. Since the Ia-positive non-B cell population is composed mostly of reticulum cells, the above findings indicate that a large number of LPC, mostly reticulum cells, not only exhibit a strong labeling with [14C]A but also express Ia antigen. The biological significance of Ia antigen expression is discussed in relation to the proliferative activity of the cells.
Osogoe
B
B
Division of Pathology, Shigei Medical Research Institute, Okayama, Japan.
Fukumoto
T
T
D000225:Adenine / Q000378:metabolism; D000818:Animals; D001485:Basement Membrane / Q000378:metabolism / Q000648:ultrastructure; D002250:Carbon Radioisotopes; D003238:Connective Tissue / Q000378:metabolism / Q000648:ultrastructure; D003239:Connective Tissue Cells; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D007070:Immunoglobulin A / Q000378:metabolism; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology* / Q000648:ultrastructure; D008297:Male; D008871:Microvilli / Q000378:metabolism / Q000648:ultrastructure; D009092:Mucous Membrane / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains
D002250:Carbon Radioisotopes; D000949:Histocompatibility Antigens Class II; D007070:Immunoglobulin A; D000225:Adenine