Roles of interleukin 1 beta and tumor necrosis factor in lipopolysaccharide fever in rats.
The roles of interleukin 1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF) in lipopolysaccharide (LPS)-induced fever were investigated in the rat. We used antisera against IL-1 beta and TNF to determine whether we could alter the fever by blocking the action of these cytokines. The intravenous injection of antiserum IL-1 beta 3.5 days before the intraperitoneal injection of LPS resulted in a mean fever that was significantly lower than that seen in rats that had been injected with control serum (0.36 +/- 0.11 vs. 0.82 +/- 0.16 degrees C, P = 0.016). The intravenous injection of antiserum against TNF 3.5 days before the intraperitoneal injection of LPS did not block the fever but significantly enhanced it (1.31 +/- 0.16 vs. 0.82 +/- 0.16 degrees C, P = 0.027). These data support the hypotheses that IL-1 beta is responsible for a significant part of LPS fever and that TNF acts as an endogenous antipyretic to limit the magnitude of LPS fever in the rat.
Long
N C
NC
Department of Physiology, University of Michigan Medical School, Ann Arbor 48109.
Otterness
I
I
Kunkel
S L
SL
Vander
A J
AJ
Kluger
M J
MJ
D000818:Animals; D001831:Body Temperature; D005334:Fever / Q000139:chemically induced / Q000503:physiopathology*; D007106:Immune Sera / Q000276:immunology; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D007375:Interleukin-1 / Q000276:immunology / Q000502:physiology*; D008070:Lipopolysaccharides; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000502:physiology*
D007106:Immune Sera; D007375:Interleukin-1; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha