Electron microscopic immunogold cytochemistry reveals chromogranin A confined to secretory granules of porcine Merkel cells.
By ultrastructural immunohistochemistry using the immunogold technique, immunoreactive (ir) Chromogranin A (CGA) was found to be confined to the secretory vesicles of porcine Merkel cells. CGA was present predominantly in the periphery of the electron-dense core and on the clear halo. These findings indicate that CGA is a regular constituent of Merkel cell secretory granules but probably not exclusively responsible for their electron opacity.
Hartschuh
W
W
Universitäts-Hautklinik, Heidelberg, F.R.G.
Weihe
E
E
Egner
U
U
D000818:Animals; D053379:Chromogranin A; D002864:Chromogranins / Q000378:metabolism*; D003594:Cytoplasmic Granules / Q000378:metabolism*; D006046:Gold; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009490:Neurosecretory Systems / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D012867:Skin / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D013552:Swine
D053379:Chromogranin A; D002864:Chromogranins; D006046:Gold