Immunogenic properties of ISCOM prepared with influenza virus nucleoprotein.
After covalent attachment of bacterial lipopolysaccharide to the nucleoprotein of influenza A virus, this water-soluble antigen could be incorporated firmly into ISCOM. This potent "immunostimulating complex" induced the production of high antibody titers in mice and could partially protect the animals from a lethal challenge infection. After immunization with ISCOM preparations NP-specific cytotoxic T cell activity could not be demonstrated.
Weiss
H P
HP
Institut für Virologie, Justus-Liebig-Universität Giessen, Federal Republic of Germany.
Stitz
L
L
Becht
H
H
D000276:Adjuvants, Immunologic; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D003601:Cytotoxicity Tests, Immunologic; D007114:Immunization; D009980:Influenza A virus / Q000276:immunology*; D008050:Lipid A; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D009698:Nucleoproteins / Q000276:immunology*; D009976:Orthomyxoviridae Infections / Q000517:prevention & control; D062605:Quillaja Saponins; D012503:Saponins; D012995:Solubility; D014758:Viral Core Proteins / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D008050:Lipid A; D008070:Lipopolysaccharides; D009698:Nucleoproteins; D062605:Quillaja Saponins; D012503:Saponins; D014758:Viral Core Proteins; C046386:Quil A