The simian immunodeficiency virus (SIV) rev gene regulates env expression.
The rev gene product is a trans-acting nuclear regulatory protein that is essential for AIDS virus replication. To define the effect of the SIV rev gene on its gp160 expression, two SV40 constructs have been made: pBC17 is a rev+env+ construct, and pBD21 is a rev-env+ construct. After transfecting the constructs into COS-1 cells, RNA, protein, syncytium formation, and monoclonal antibody blocking assays were performed. The results indicated that the SIV rev gene positively influenced the level of full-length env mRNA and was required for expression of SIV gp160.
Cheng
S M
SM
Biotechnology and Microbiology Division, Wyeth-Ayerst Research, Radnor, PA.
Blume
M
M
Lee
S G
SG
Hung
P P
PP
Hirsch
V M
VM
Johnson
P R
PR
D000911:Antibodies, Monoclonal; D015152:Blotting, Northern; D002460:Cell Line; D003001:Cloning, Molecular; D015967:Gene Expression Regulation, Viral; D015686:Gene Products, env / Q000235:genetics* / Q000378:metabolism; D015752:Genes, env; D015757:Genes, rev; D015726:Giant Cells / Q000378:metabolism; D019302:HIV Envelope Protein gp160; D006801:Humans; D010957:Plasmids; D011233:Precipitin Tests; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D015302:Simian Immunodeficiency Virus / Q000235:genetics*; D015533:Transcriptional Activation; D014162:Transfection
D000911:Antibodies, Monoclonal; D015686:Gene Products, env; D019302:HIV Envelope Protein gp160; D011498:Protein Precursors; D012333:RNA, Messenger; D012367:RNA, Viral