Release of T-antigen, a carcinoma marker from native human cells, by endo-alpha-N-acetylgalactosaminidase of Alcaligenes sp.
The endo-alpha-N-acetylgalactosaminidase from Alcaligenes sp. could release T-antigen (Ga1 beta 1----3GalNAc), a carcinoma-associated marker from asialo glycophorin and human cells. The released T-antigen from human asialo erythrocytes was determined by thin layer chromatography and gas liquid chromatography. The released T-antigen from human gastric carcinoma cell Kato III was identified by high performance liquid chromatographies with a reverse-phase column and a size fractionation column. Released T-antigen could be analyzed quantitatively by a sensitive method including pyridylamino derivatization and following high performance liquid chromatography. This suggests that the enzyme is useful for detection and determination of T-antigen from cells.
Fan
J Q
JQ
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan.
Kanatani
K
K
Andoh
N
N
Yamamoto
K
K
Kumagai
H
H
Tochikura
T
T
D000421:Alcaligenes / Q000201:enzymology*; D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor / Q000032:analysis*; D002460:Cell Line; D002846:Chromatography, Affinity; D004187:Disaccharides / Q000032:analysis*; D004912:Erythrocytes / Q000276:immunology; D006021:Glycophorins / Q000302:isolation & purification; D006596:Hexosaminidases / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D013274:Stomach Neoplasms; D048809:alpha-N-Acetylgalactosaminidase
D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor; D004187:Disaccharides; D006021:Glycophorins; C016467:Thomsen-Friedenreich antigen; D006596:Hexosaminidases; C482722:NAGA protein, human; D048809:alpha-N-Acetylgalactosaminidase