HAB-1, a new heteromyeloma for continuous production of human monoclonal antibodies.
To obtain suitable cell lines for the immortalisation of human lymphocytes, we constructed a heteromyeloma between the murine myeloma Ag8 and human lymphocytes from a highly malignant polymorphic, centroblastic B-cell lymphoma. The thioguanine-resistant and HAT-sensitive heteromyeloma HAB-1 neither secretes nor contains cytoplasmatic immunoglobulins, the cells being EBV negative but positively stained for HLA-BC and the human proliferation marker Ki-67. The karyotype consists of about 50 murine and 20 human chromosomes. The HAB-1 cells grow in suspension and have a doubling rate of about 25-30 h. In fusion experiments with spleen cells from stomach carcinoma patients HAB-1 cells show a 5-7 times higher fusion efficiency than murine Ag8 cells or another heteromyeloma SPM4-0 and give stable antibody producing products. The cell line will be made available to interested scientists.
Faller
G
G
Institut für Pathologie, Universität Würzburg, FR Germany.
Vollmers
H P
HP
Weiglein
I
I
Marx
A
A
Zink
C
C
Pfaff
M
M
Müller-Hermelink
H K
HK
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D015703:Antigens, CD / Q000032:analysis; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007621:Karyotyping; D016393:Lymphoma, B-Cell / Q000276:immunology; D051379:Mice; D009101:Multiple Myeloma / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD