[Isoenzyme spectrum and kinetic properties of pyruvate kinase from the liver of thiamine-deficient rats].
Thiamine-deficiency in animals induced by everyday subcutaneous administration of oxythiamine in a dose of 4, 40 and 100 mg/kg of weight for 10 days results in a decrease of the total activity of pyruvate kinase in the liver tissue and does not affect the mentioned index in the kidney and heart tissues. It is shown that as a result of the enzyme fractionation in the column with DEAE-cellulose the total activity of pyruvate kinase in the liver tissue of rats with thiamine-deficiency decreases due to L-isoform while the content of M-isoform remains unchanged. Thiamine deficiency does not affect kinetic characteristics of the L-isoform, extracted from the liver and this shows the absence of changes in the degree of phosphorylation of pyruvate kinase L-isoform under these conditions.
Konovalenko
O V
OV
Maglysh
S S
SS
Gorbach
Z V
ZV
D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D007527:Isoenzymes / Q000037:antagonists & inhibitors* / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D009206:Myocardium / Q000201:enzymology; D009928:Organ Specificity; D011770:Pyruvate Kinase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051381:Rats; D013832:Thiamine Deficiency / Q000201:enzymology*
D007527:Isoenzymes; D011770:Pyruvate Kinase
Izofermentnyĭ spektr i kineticheskie svoĭstva piruvatkinazy pecheni tiamindefitsitnykh krys.