[Ligand-exchange chromatography of proteins and enzymes].
Current state of the ligand-exchange chromatography (metal chelate affinity chromatography) of proteins and enzymes is reviewed. This technique is based on the ability of proteins to bind metal ions immobilized on chelate gels. The influence of pH, composition of buffer, type of stationary ligand and nature of metal ions on the chromatographic behaviour of proteins is discussed.
Lopatin
S A
SA
Varlamov
V P
VP
Davankov
V A
VA
D002846:Chromatography, Affinity / Q000379:methods*; D004798:Enzymes / Q000032:analysis*; D008024:Ligands; D011506:Proteins / Q000032:analysis*
D004798:Enzymes; D008024:Ligands; D011506:Proteins
Ligandoobmennaia khromatografiia belkov i fermentov.