Salmonella-reactive synovial fluid T-cell clones in a patient with post-infectious Salmonella arthritis.
From a patient with reactive arthritis following Salmonella typhimurium enteritis, synovial fluid T-lymphocytes were cloned and expanded in vitro. Seven out of 74 clones showed a marked proliferative response to antigens of heat-killed Salmonella typhimurium with autologous T-cell-depleted peripheral blood mononuclear cells as antigen-presenting cells. The Salmonella-reactive clones were of the CD4+ phenotype, antigen-induced proliferation could be inhibited by a monoclonal antibody to HLA class II. One clone recognized both Salmonella and Campylobacter jejuni antigens in the proliferation assay. The multiclonality of Salmonella-reactive synovial fluid T-cells indicates that the microorganisms have been present, at least transiently, within the affected joint and thus recruited specific T-lymphocytes that might initiate synovitis.
Hermann
E
E
Department of Internal Medicine, Johannes Gutenberg University, Mainz, FRG.
Mayet
W J
WJ
Poralla
T
T
Meyer zum Büschenfelde
K H
KH
Fleischer
B
B
D000328:Adult; D001168:Arthritis / Q000209:etiology / Q000276:immunology*; D001773:Blood Cells / Q000276:immunology; D002455:Cell Division; D002999:Clone Cells; D006801:Humans; D008297:Male; D012475:Salmonella / Q000276:immunology*; D012480:Salmonella Infections / Q000276:immunology*; D012486:Salmonella typhimurium / Q000276:immunology; D013582:Synovial Fluid / Q000166:cytology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*