title: [Reconstruction of the synthetic gene for human interleukin-2].
abstract: Chemical-enzymatic synthesis and cloning of the DNA fragment coding for the human interleukin-2 signal sequence was accomplished. A hybrid plasmid pSIL-2 containing the gene of the human interleukin-2 with this signal sequence was constructed for effective expression of the gene in eukaryotic systems. A variant permitting the removal of the interleukin-2 stop-codons was obtained, which is suitable for the construction of chimeric genes containing the interleukin-2 gene sequence at the 5'-end.
authors.forename: S V
authors.initials: SV
authors.lastname: Seregin
authors.forename: V A
authors.initials: VA
authors.lastname: Riabinin
authors.forename: A N
authors.initials: AN
authors.lastname: Siniakov
authors.forename: N K
authors.initials: NK
authors.lastname: Daniliuk
authors.forename: S G
authors.initials: SG
authors.lastname: Pozdniakov
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D015252:Deoxyribonucleases, Type II Site-Specific; D015870:Gene Expression; D005813:Genes, Synthetic; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010957:Plasmids
chemical_list: D004274:DNA, Recombinant; D007376:Interleukin-2; D015345:Oligonucleotide Probes; C035042:endodeoxyribonuclease FokI; D015252:Deoxyribonucleases, Type II Site-Specific
vernacular_title: Rekonstruktsiia iskusstvennogo gena chelovecheskogo interleĭkina-2.