title: A diagnostic method to detect alcelaphine herpesvirus-1 of malignant catarrhal fever using the polymerase chain reaction.
abstract: A sensitive diagnostic method specific for alcelaphine herpesvirus-1, causative agent of malignant catarrhal fever, has been developed. Based on the nucleotide sequence of the alcelaphine herpesvirus-1 genomic DNA, a pair of 30 nucleotide primers was selected and synthesized for detecting the virus genome using the polymerase chain reaction (PCR). The virus genome was detected in crude cell lysate using the amplification reaction.
authors.affiliation: Department of Veterinary Microbiology and Immunology, University of California, Davis.
authors.forename: D
authors.initials: D
authors.lastname: Hsu
authors.forename: L M
authors.initials: LM
authors.lastname: Shih
authors.forename: A E
authors.initials: AE
authors.lastname: Castro
authors.forename: Y C
authors.initials: YC
authors.lastname: Zee
mesh_terms: D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014709:Vero Cells
chemical_list: D004279:DNA, Viral