N-myc suppression and cell cycle arrest at G1 phase by prostaglandins.
Effects of cyclopentenone prostaglandins, delta 12-prostaglandin (PG) J2 and PGA2 on the expression of N-myc in relation to the effects on cell cycle progression were investigated using human neuroblastoma cell line GOTO. Both PGs suppressed N-myc expression within several hours prior to inducing G1 arrest. The N-myc suppression with delta 12-PGJ2 was continued but with PGA2 it was gradually released, followed by the release of G1 arrest. These results suggest that delta 12-PGJ2 and PGA2 inhibit cell cycle progression in strong association with N-myc suppression and delta 12-PGJ2 is more potent and has a longer effect than PGA2.
Marui
N
N
Department of Preventive Medicine, Kyoto Prefectural University of Medicine, Japan.
Sakai
T
T
Hosokawa
N
N
Yoshida
M
M
Aoike
A
A
Kawai
K
K
Nishino
H
H
Fukushima
M
M
D002453:Cell Cycle / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D016259:Genes, myc; D006801:Humans; D009447:Neuroblastoma; D015230:Prostaglandin D2 / Q000494:pharmacology* / Q000031:analogs & derivatives; D011454:Prostaglandins A / Q000494:pharmacology*; D011465:Prostaglandins, Synthetic / Q000494:pharmacology*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D013489:Suppression, Genetic; D014407:Tumor Cells, Cultured
D011454:Prostaglandins A; D011465:Prostaglandins, Synthetic; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; C037112:9-deoxy-delta-9-prostaglandin D2; C100008:prostaglandin A2; D015230:Prostaglandin D2