A stalk-specific wheat germ agglutinin binding protein, wst34, in Dictyostelium discoideum can be detected with antiserum raised against Dictyostelium mucoroides stalk.
A new stalk-specific wheat germ agglutinin (WGA) binding protein, wst34, has been identified in Dictyostelium discoideum and purified by the use of preparative sodium dodecyl sulfate - polyacrylamide gel electrophoresis and a WGA-affinity column. In normal development, wst34 appears during culmination and is maintained in stalk cells. It has a molecular mass of 34 kilodaltons and a pI value of 5.5-6.5. A polyclonal antiserum raised against stalk cell proteins of Dictyostelium mucoroides recognizes wst34 in western blots of D. discoideum proteins.
Kubohara
Y
Y
Department of Botany, Faculty of Science, Kyoto University, Japan.
Okamoto
K
K
D015153:Blotting, Western; D002352:Carrier Proteins / Q000032:analysis* / Q000276:immunology; D002454:Cell Differentiation; D004023:Dictyostelium / Q000032:analysis* / Q000254:growth & development / Q000276:immunology; D005656:Fungal Proteins / Q000032:analysis* / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D008970:Molecular Weight; D013045:Species Specificity; D014909:Wheat Germ Agglutinins / Q000378:metabolism*
D002352:Carrier Proteins; D005656:Fungal Proteins; D007106:Immune Sera; D014909:Wheat Germ Agglutinins