title: Failure to detect IL-3-binding sites on human mast cells.
abstract: IL-3, a pleiotropic lymphokine, has been termed mast cell growth factor because it promotes growth and differentiation of murine mast cells. Murine mast cells, in turn, express cell surface receptors for IL-3. Human rIL-3 has been shown to induce proliferation and differentiation of human basophils and to activate basophils via high affinity binding sites. To investigate whether human mast cells express IL-3R, binding studies with 125I-radiolabeled human rIL-3 were performed on HMC-1, a novel human mast cell line, and on pure populations (i.e., 93 to 99% purity) of human tissue mast cells obtained with mAb and C from dispersed lung (n = 2). Unexpectedly, neither enriched human lung mast cells nor HMC-1 cells bound radiolabeled human rIL-3 specifically. Moreover, human rIL-3 failed to promote uptake of [3H]thymidine, synthesis of histamine, histamine releasability, or changes in expression of mast cell differentiation Ag (YB5B8, CD54/ICAM-1, CD9/p24, CD33/gp67) on either human lung mast cells or HMC-1 cells. It is hypothesized that the fundamental difference in the biologic response to IL-3 between human and murine mast cells is due to a loss during evolution of mast cell high affinity IL-3 binding sites.
authors.affiliation: I. Medical Department, University of Vienna, Austria.
authors.forename: P
authors.initials: P
authors.lastname: Valent
authors.forename: J
authors.initials: J
authors.lastname: Besemer
authors.forename: C
authors.initials: C
authors.lastname: Sillaber
authors.forename: J H
authors.initials: JH
authors.lastname: Butterfield
authors.forename: R
authors.initials: R
authors.lastname: Eher
authors.forename: O
authors.initials: O
authors.lastname: Majdic
authors.forename: K
authors.initials: K
authors.lastname: Kishi
authors.forename: W
authors.initials: W
authors.lastname: Klepetko
authors.forename: F
authors.initials: F
authors.lastname: Eckersberger
authors.forename: K
authors.initials: K
authors.lastname: Lechner
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D001491:Basophils / Q000187:drug effects / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002469:Cell Separation; D006801:Humans; D007377:Interleukin-3 / Q000378:metabolism* / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D016185:Receptors, Interleukin-3 / Q000032:analysis*; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D015703:Antigens, CD; D007377:Interleukin-3; D016185:Receptors, Interleukin-3; D011994:Recombinant Proteins