title: Proteolytic activity in mouse urine: relationship to the kidney metallo-endopeptidase, meprin.
abstract: Meprin, a brush border kidney metallo-endopeptidase is present as the major endopeptidase in mouse urine. The enzyme is freely soluble and can be detected enzymically or immunologically. Mice can be partitioned into two phenotypes that differ by 10-20-fold in the amount of meprin in kidney membranes; this phenotypic variation is reflected in urinary activities. We propose a role for meprin in the degradation of other urinary proteins.
authors.affiliation: Department of Biochemistry, University of Liverpool, U.K.
authors.forename: A V
authors.initials: AV
authors.lastname: Flannery
authors.forename: G N
authors.initials: GN
authors.lastname: Dalzell
authors.forename: R J
authors.initials: RJ
authors.lastname: Beynon
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000302:isolation & purification / Q000652:urine*; D006020:Glycopeptides / Q000494:pharmacology; D007328:Insulin; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D008666:Metalloendopeptidases / Q000378:metabolism* / Q000652:urine; D051379:Mice; D008815:Mice, Inbred Strains; D011480:Protease Inhibitors / Q000494:pharmacology; D013379:Substrate Specificity
chemical_list: D006020:Glycopeptides; D007328:Insulin; D011480:Protease Inhibitors; D010450:Endopeptidases; D008666:Metalloendopeptidases; C053833:meprin A; C008890:phosphoramidon