Immunization against recombinant bovine inhibin alpha subunit causes increased ovulation rates in gilts.
Immunization of gilts in a commercial piggery against a fusion protein of the alpha subunit of bovine inhibin, produced by recombinant DNA methods, resulted in mean ovulation rate increases of 35% at the oestrus at which, under the piggery's management practices, they would have been mated. Sera from two immunized groups showed mean binding of 6.6% and 4.9% when assayed, at 1:800 final dilution, against iodinated bovine inhibin (Mr 31,000). Ovulation rates of immunized gilts were highly correlated with the ability of serum to bind iodinated native inhibin (r = 0.62; P less than 0.001), particularly when weight and age were included in the correlation (r = 0.72; P = 0.001), and inhibin binding accounted for 38% of the total variation in ovulation rate. Immunization caused no deleterious effects on growth rate or onset of oestrus. These results demonstrate the potential for use of such immunization to increase prolificacy in gilts and young sows.
Brown
R W
RW
Biotech Australia Pty Ltd, East Roseville, New South Wales, Australia.
Hungerford
J W
JW
Greenwood
P E
PE
Bloor
R J
RJ
Evans
D F
DF
Tsonis
C G
CG
Forage
R G
RG
D000818:Animals; D002417:Cattle; D005260:Female; D007114:Immunization; D007265:Inhibins / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D010060:Ovulation / Q000502:physiology*; D011485:Protein Binding; D011993:Recombinant Fusion Proteins; D013552:Swine / Q000502:physiology*
D011993:Recombinant Fusion Proteins; D007265:Inhibins