[Use of fluorescence polarization immunoassay and microbiological assay in the study of gentamicin pharmacokinetics in man and a comparison of the results].
Ninety serum samples of gentamicin were determined by fluorescence polarization immunoassay (FPIA) and microbiological assay (MA) and the results were compared. Liner regression analysis showed that the two methods were highly correlated with a correlation coefficient of 0.98. The mean concentration of gentamicin as determined by MA method was significantly higher than that by FPIA method. Serum concentration-time data derived from 5 patients were fitted to a one-compartment model. No significant differences in the pharmacokinetic parameters and estimated dosages were observed. The FPIA method was shown to be as correct and reliable as the MA method in estimating gentamicin pharmacokinetic parameters.
Cai
W M
WM
General Hospital of Nanjing Armed Forces.
Chen
G
G
D000005:Abdomen / Q000601:surgery; D005260:Female; D005454:Fluorescence Polarization; D005839:Gentamicins / Q000493:pharmacokinetics*; D006801:Humans; D007118:Immunoassay; D008297:Male; D008828:Microbiological Techniques; D011184:Postoperative Period
D005839:Gentamicins