title: Lack of formyl-methionyl-leucyl-phenylalanine receptors on porcine neutrophils.
abstract: The response of blood neutrophils to the chemotactic peptide formyl-methyl-leucyl-phenylalanine varies among species. Our results indicate that this peptide does not activate the respiratory burst of porcine neutrophils. Specifically, concentrations less than or equal to 10(-6) M did not cause production of either superoxide or hydrogen peroxide. Studies designed to delineate the biochemical deficit responsible for these results indicated that these cells do not express specific chemotactic peptide receptors on the external surface of the plasma membrane. Although these data do not rule out the possibility that internal stores of chemotactic peptide receptor exist, attempts to induce expression of the receptor by priming the cells with either lipopolysaccharide or calcium ionophore were unsuccessful.
authors.affiliation: Department of Biomedical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg 24061.
authors.forename: F Y
authors.initials: FY
authors.lastname: el-Awar
authors.forename: D L
authors.initials: DL
authors.lastname: Ochs
authors.forename: R H
authors.initials: RH
authors.lastname: Pyle
authors.forename: H P
authors.initials: HP
authors.lastname: Misra
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002469:Cell Separation / Q000379:methods / Q000662:veterinary; D005260:Female; D005434:Flow Cytometry / Q000379:methods / Q000662:veterinary*; D008297:Male; D009504:Neutrophils / Q000378:metabolism*; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic / Q000378:metabolism*; D013552:Swine / Q000097:blood*
chemical_list: D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic; D002118:Calcium