Retinoic acid inhibits the myristoylation of a membrane protein in HL-60 cells.
Incubation of nondifferentiated HL-60 cells with high specific activity myristic acid results in myristoylation of a 25 KD membrane protein. Myristoylation was inhibited by retinoic acid but not by DMSO, thus indicating that the nonmyristoylated state induced by retinoic acid is causal to rather than the result of the overall differentiation sequel induced. Similarly, incubation of HL-60 cells with high specific activity retinoic acid results in specific retinoylation of a membrane protein of similar molecular mass. The two acylated proteins could however be separated by 2-D gel electrophoresis, thus indicating that inhibition of myristoylation by retinoic acid could not be accounted for by competition between retinoic and myristic acids for the same acylated site.
Almagor
M
M
Department of Biochemistry, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
Bar-Tana
J
J
D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D019814:Myristic Acid; D009227:Myristic Acids / Q000378:metabolism*; D014212:Tretinoin / Q000378:metabolism / Q000494:pharmacology*
D008565:Membrane Proteins; D009227:Myristic Acids; D019814:Myristic Acid; D014212:Tretinoin; D004121:Dimethyl Sulfoxide