"Pure" human hematopoietic progenitors: permissive action of basic fibroblast growth factor.
Methodology has been developed that enables virtually complete purification and abundant recovery of early hematopoietic progenitors from normal human adult peripheral blood. A fraction of the pure progenitors is multipotent (generates mixed colonies) and exhibits self-renewal capacity (gives rise to blast cell colonies). This methodology provides a fundamental tool for basic and clinical studies on hematopoiesis. Optimal in vitro cloning of virtually pure progenitors requires not only the stimulatory effect of interleukin-3, granulocyte-macrophage colony-stimulating factor, and erythropoietin, but also the permissive action of basic fibroblast growth factor. These findings suggest a regulatory role for this growth factor in early hematopoiesis.
Gabbianelli
M
M
Department of Hematology and Oncology, Istituto Superiore di Sanità, Rome, Italy.
Sargiacomo
M
M
Pelosi
E
E
Testa
U
U
Isacchi
G
G
Peschle
C
C
D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D002469:Cell Separation; D002478:Cells, Cultured; D002999:Clone Cells; D004921:Erythropoietin / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology; D009000:Monocytes / Q000166:cytology* / Q000187:drug effects; D011994:Recombinant Proteins / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D007377:Interleukin-3; D011994:Recombinant Proteins; D016222:Fibroblast Growth Factor 2; D004921:Erythropoietin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor