Microsequencing of proteins electrotransferred onto immobilizing matrices from polyacrylamide gel electrophoresis: application to an insoluble protein.
Semidry electroblotting is convenient and allows a rapid and efficient protein transfer from one- or two-dimensional polyacrylamide gels onto sequencer stable supports for protein microsequence analysis in a gas-phase sequencer. Using this technique, we determined the amino acid sequences of the basic 7S globulin (Bg), an insoluble protein present in soybean seeds. Based on sequence determination, the cDNA-encoding Bg could be easily cloned and characterized.
Hirano
H
H
National Institute of Agrobiological Resources, Tsukuba, Japan.
Watanabe
T
T
D000595:Amino Acid Sequence; D052179:Antigens, Plant; D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005916:Globulins / Q000235:genetics*; D007768:Lactalbumin / Q000737:chemistry; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D055314:Seed Storage Proteins; D012639:Seeds / Q000235:genetics; D012995:Solubility; D030262:Soybean Proteins; D013025:Glycine max / Q000235:genetics*
D052179:Antigens, Plant; D005916:Globulins; D012333:RNA, Messenger; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; D004247:DNA; D007768:Lactalbumin