Lymphocyte activation as measured by interleukin-2 receptor expression to gluten fraction 111 in coeliac disease.
Lymphocyte activation was examined by interleukin-2 (IL-2) receptor expression on peripheral blood mononuclear cells from coeliac and control subjects. Purified T cells were incubated with gluten fraction 111 (a known toxic peptide for coeliac subjects), soyabean hydrolysate (an unrelated hydrolysed food antigen), and Concanavalin-A (Con-A, a non-specific mitogen). After 1-5 days incubation, expression of IL-2 receptors was assessed using a cellular enzyme-linked immunosorbent assay (ELISA). Gluten fraction 111 induced expression of IL-2 receptors on T lymphocytes from coeliac but not from normal subjects (P = 0.0005), whereas soyabean hydrolysate did not induce IL-2 receptor expression. Lymphocytes from both coeliac and normal subjects had similar increased IL-2 receptor expression after incubation with Con-A. Flow cytometry was also used to confirm specific expression of IL-2 receptor expression of lymphocytes from coeliac subjects. Interleukin-2 receptor expression increased from 0 to 5.4% of cultured mononuclear cells after 7 days incubation with gluten fraction III. These cells were CD3-positive and CD4-positive. We conclude that peripheral blood lymphocytes from coeliac subjects are sensitized specifically to gluten fraction III.
Penttila
I A
IA
Department of Medicine, Royal Adelaide Hospital, Australia.
Gibson
C E
CE
Forrest
B D
BD
Cummins
A G
AG
LaBrooy
J T
JT
D000704:Analysis of Variance; D002446:Celiac Disease / Q000276:immunology*; D002478:Cells, Cultured; D003208:Concanavalin A / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005983:Glutens / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology*; D014674:Plant Proteins, Dietary / Q000276:immunology; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis*; D030262:Soybean Proteins; D013601:T-Lymphocytes / Q000276:immunology*
D014674:Plant Proteins, Dietary; D015375:Receptors, Interleukin-2; D030262:Soybean Proteins; D003208:Concanavalin A; D005983:Glutens