Polyacrylamide gel analysis of polypeptides in gene transfer cell lines.
Differential polypeptide expression in gene transfer cell lines of limited genetic complexity was analyzed as a gene mapping strategy. Subcellular fractionation preceding two-dimensional gel electrophoretic analysis simplified protein patterns and revealed subcellular location of differentially expressed polypeptides. As a model system, human MET oncogene polypeptide was identified in gene transfer lines by this approach. Genes encoding five putative human proteins were identified and provisionally assigned to chromosomal region 7q21-31 or to chromosome 1.
Strong
T V
TV
Department of Pathology, University of Alabama, Birmingham 35294.
Barker
P E
PE
Anderson
S J
SJ
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D002897:Chromosomes, Human, Pair 7; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007553:Isotope Labeling; D051379:Mice; D008970:Molecular Weight; D010455:Peptides / Q000235:genetics*; D011499:Protein Processing, Post-Translational; D013347:Subcellular Fractions; D014162:Transfection
D000596:Amino Acids; D010455:Peptides