[Quantitative studies of mast cell proliferation at the wound edge --rate of DNA synthesis in intravital and postmortem biopsy].
The number of DNA synthesizing mast cells in experimental induced wounds was calculated using bromodeoxyuridine as a DNA marker and naphthol AS-D chloroacetate esterase as a mast cell marker. After different survival times, specimens of the wound edge were taken before death and 24 hs after death. At a survival time of 48 hs in vital biopsy the number of DNA synthesizing mast cells peaked, at 72 hs and fell after a period of 8 days. Even though an identical kinetic course was demonstrable in cases of postmortal biopsies the percentage at each survival period was significantly lower than in vital biopsies.
Oehmichen
M
M
Institut für Rechtsmedizin, Universität zu Köln.
Cröpelin
A
A
Schmidt
V
V
D000818:Animals; D002455:Cell Division / Q000502:physiology*; D004261:DNA Replication / Q000502:physiology*; D004431:Ear, External / Q000293:injuries; D008407:Mast Cells / Q000473:pathology*; D051381:Rats; D014945:Wound Healing / Q000502:physiology*; D014950:Wounds, Penetrating / Q000473:pathology
Quantitative Untersuchungen zur Mastzellproliferation am Wundrand--DNS-Syntheserate bei intravitaler und postmortaler Biopsie.