title: DNA typing for HLA-DPB1*02 and -DPB1*04 in multiple sclerosis and juvenile rheumatoid arthritis.
abstract: DP gene typing using in vitro DNA amplification combined with sequence-specific oligonucleotide probes (SSOP) has recently been reported. The amplification step may be specific for the HLA-DPB locus, or it may be specific for one or a group of HLA-DPB alleles, thus increasing the discriminatory power of the system. We report the combined use of group-specific DNA in vitro amplification followed by SSOP in typing for DPB1*02 and DPB1*04 variants. The method was used to type for these variants in 96 randomly selected, healthy Danes, in 37 patients with pauciarticular juvenile rheumatoid arthritis (PJRA), and in 38 patients with multiple sclerosis (MS). Increased frequencies of the cellularly defined HLA-DPw2 in PJRA and of HLA-DPw4 in MS have previously been reported. In the patient groups, the frequencies of the DPB1*02 and DPB1*04 variants did not differ significantly from those expected based on the cellularly defined HLA-DP types of the patients and the frequencies of the DPB1*02 and DPB1*04 variants among healthy Danes.
authors.affiliation: Department of Clinical Immunology, State University Hospital, Copenhagen, Denmark.
authors.forename: L
authors.initials: L
authors.lastname: Fugger
authors.forename: L P
authors.initials: LP
authors.lastname: Ryder
authors.forename: N
authors.initials: N
authors.lastname: Morling
authors.forename: N
authors.initials: N
authors.lastname: Odum
authors.forename: J
authors.initials: J
authors.lastname: Friis
authors.forename: F K
authors.initials: FK
authors.lastname: Pedersen
authors.forename: C
authors.initials: C
authors.lastname: Heilmann
authors.forename: M
authors.initials: M
authors.lastname: Sandberg-Wollheim
authors.forename: A
authors.initials: A
authors.lastname: Svejgaard
mesh_terms: D000595:Amino Acid Sequence; D001171:Arthritis, Juvenile / Q000235:genetics*; D001483:Base Sequence; D005784:Gene Amplification; D005787:Gene Frequency; D006682:HLA-DP Antigens / Q000032:analysis / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009103:Multiple Sclerosis / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
chemical_list: D006682:HLA-DP Antigens; D015345:Oligonucleotide Probes