Inhibitory effect of bestatin on the growth of human leukemic cells.
We examined the effect of bestatin (Ubenimex) on the growth of human leukemic cells (i.e, HL-60, K562, MT-1, MT-2, Molt-4, and Raji cells). The growth of each cell line was inhibited by the cocultivation with bestatin at higher concentrations than employed for clinical use in Japan. [3H]TdR incorporation was also inhibited in MT-1 and MT-2 cells by treatment with bestatin. Degenerated cell-to-cell adhesion was observed among the treated cells. These findings suggest that the inhibitory effect on some leukemic cells, especially on MT-1 cells, results from the inhibition of DNA synthesis.
Sakurada
K
K
Third Department of Internal Medicine, Hokkaido University, School of Medicine, Sapporo, Japan.
Imamura
M
M
Kobayashi
M
M
Tachibana
N
N
Abe
K
K
Tanaka
M
M
Okabe
M
M
Morioka
M
M
Kasai
M
M
Sugiura
T
T
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000187:drug effects / Q000648:ultrastructure; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007930:Leucine / Q000031:analogs & derivatives* / Q000494:pharmacology; D007938:Leukemia / Q000473:pathology*; D008855:Microscopy, Electron, Scanning; D013601:T-Lymphocytes / Q000187:drug effects / Q000648:ultrastructure; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000903:Antibiotics, Antineoplastic; D007930:Leucine; C012211:ubenimex