Flow cytometry (FCM) analysis of endometrial hyperplasia and carcinoma.
In a series of 52 biopsy specimens (31 endometrial carcinomas, 10 atypical endometrial hyperplasias, and 11 cases of normal endometrium), DNA ploidy and S-phase cell fraction were estimated in paraffin-embedded material. DNA aneuploidy was detected in 2 of the 10 atypical endometrial hyperplasias and 7 of the 31 endometrial carcinomas. The majority of aneuploidy was found to be connected with the loss of tumor differentiation. No ploidy disturbances were found in normal endometrium. The S-phase cell fraction value of normal endometrium was significantly lower when compared with that of endometrial carcinoma. The broad variation in S-phase cell fraction values of the endometrial carcinomas and atypical endometrial hyperplasias was in contrast with the low variability of S-phase cell values of normal endometrium. Very low incidence of aneuploidy in the group of well differentiated endometrial carcinomas (Grade I) enables the suggestion that the presence of aneuploidy predicts a more aggressive disease and that the detection of an aneuploid stemline in atypical endometrial hyperplasia may already indicate the neoplastic transformation.
Kysela
B
B
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Siracký
J
J
Redecha
M
M
Bárdos
A
A
D002471:Cell Transformation, Neoplastic; D004247:DNA / Q000032:analysis*; D004714:Endometrial Hyperplasia / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D011003:Ploidies; D012189:Retrospective Studies; D016196:S Phase; D014594:Uterine Neoplasms / Q000235:genetics*
D004247:DNA