Mouse mammary carcinoma delta-aminolevulinate dehydratase.
1. Aminolevulinate dehydratase (ALA-D) was studied in crude extract from mouse mammary carcinoma, normal mouse liver and tumour bearing mouse liver. 2. A Michaelis-Menten behaviour and Km values between 0.24 and 0.31 mM were obtained for the enzyme in either source. 3. In all three tissues there was a linear relationship between porphobilinogen formation and incubation time, up to 120 min, ALA-D was thermostable and optimum pH was at 6.8. 4. There seems to be no structural alterations in tumoural ALA-D as compared with the enzyme from liver of both normal and tumour bearing mice.
Navone
N M
NM
Centro de Investigaciones sobre Porfirinas y Porfirias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Polo
C F
CF
Frisardi
A L
AL
Batlle
A M
AM
D000818:Animals; D004795:Enzyme Stability; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000201:enzymology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011162:Porphobilinogen / Q000378:metabolism; D000623:Porphobilinogen Synthase / Q000378:metabolism*
D011162:Porphobilinogen; D000623:Porphobilinogen Synthase