title: Changes in calcitonin gene-related peptide immunoreactivity in the rat dorsal horn following electrical stimulation of the sciatic nerve.
abstract: Immunoreactive staining for calcitonin gene-related peptide (CGRP) in the rat superficial dorsal horn (L4-L5 segments) was examined following electrical stimulation of the sciatic nerve. Electrical stimulation for twenty minutes at an intensity to elicit both A and C fiber volleys resulted in a significant decrease in CGRP immunoreactivity in the medial two-thirds of the dorsal horn on the stimulated side as compared to the non-stimulated side of the spinal cord. The change was most pronounced in the medial area of the dorsal horn, with a 54% decrease in immunostaining density compared to the non-stimulated side. In contrast, when the sciatic nerve was stimulated for the same period at an intensity that stimulates only A fibers, there was no significant difference in the density of immunoreactive staining in the dorsal horn between the non-stimulated and stimulated sides of the spinal cord. These results suggest that activity in a specific population of primary afferent fibers causes a dramatic depletion of CGRP in the dorsal horn.
authors.affiliation: Department of Anatomy and Neuroscience, University of Texas Medical Branch, Galveston 77550.
authors.forename: C M
authors.initials: CM
authors.lastname: Klein
authors.forename: R E
authors.initials: RE
authors.lastname: Coggeshall
authors.forename: S M
authors.initials: SM
authors.lastname: Carlton
authors.forename: K N
authors.initials: KN
authors.lastname: Westlund
authors.forename: L S
authors.initials: LS
authors.lastname: Sorkin
mesh_terms: D000344:Afferent Pathways / Q000502:physiology; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012584:Sciatic Nerve / Q000502:physiology*; D013116:Spinal Cord / Q000378:metabolism* / Q000502:physiology
chemical_list: D015740:Calcitonin Gene-Related Peptide