Direct enzyme-linked immunosorbent assay of anti-peptide antibodies using capture of biotinylated peptides by immobilized avidin.
Synthetic peptides were prepared by a solid-phase method and biotinylated selectively and in high yield at the amino terminus prior to peptide deprotection and detachment from synthesis resin. It was shown that peptides biotinylated in this manner could be bound by avidin immobilized on a plastic surface and used to detect anti-peptide antibodies in an enzyme-linked immunosorbent assay. The advantages of this method compared to conventional immunoassay techniques for anti-peptide antibodies are discussed.
Fischer
P M
PM
Department of Biological Sciences, Deakin University, Geelong, Victoria, Australia.
Howden
M E
ME
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D001360:Avidin; D001710:Biotin; D003432:Cross-Linking Reagents; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D013744:Tetanus Toxin / Q000276:immunology
D000906:Antibodies; D003432:Cross-Linking Reagents; D010455:Peptides; D013744:Tetanus Toxin; D001360:Avidin; D001710:Biotin