title: Developmentally regulated fetal thymic and extrathymic T-cell receptor gamma delta gene expression.
abstract: The gamma delta T-cell receptor (TCR) is the first TCR to be expressed in ontogeny in all vertebrates in which it has been examined thoroughly. Murine gamma delta cell-surface protein is detected by the fourteenth day of gestation. In this work, the activation of gamma delta RNA has been studied. Data indicate that the first TCR protein to appear in the thymus is encoded by gamma genes that are activated after cells colonize the thymus. However, the sequential appearance of different gamma delta TCR proteins during thymic ontogeny cannot be readily explained by differential temporal activation of V gamma genes in the thymus. There are distinct patterns of gamma and delta gene expression during fetal liver development and in the fetal gut (or tissue associated with it). Cells apparent in the liver of mice at birth express gamma delta cell-surface protein, but they disappear from the liver very soon afterward. One V gamma gene is rearranged and expressed prethymically. In addition, gamma gene expression is detectable in the livers of newborn athymic mice. Together, these observations indicate a thymic-independent pathway of activation of TCR genes.
authors.affiliation: Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut.
authors.forename: S R
authors.initials: SR
authors.lastname: Carding
authors.forename: S
authors.initials: S
authors.lastname: Kyes
authors.forename: E J
authors.initials: EJ
authors.lastname: Jenkinson
authors.forename: R
authors.initials: R
authors.lastname: Kingston
authors.forename: K
authors.initials: K
authors.lastname: Bottomly
authors.forename: J J
authors.initials: JJ
authors.lastname: Owen
authors.forename: A C
authors.initials: AC
authors.lastname: Hayday
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000235:genetics; D015139:Blotting, Southern; D016207:Cytokines / Q000096:biosynthesis; D004064:Digestive System / Q000196:embryology / Q000378:metabolism; D005786:Gene Expression Regulation; D015329:Gene Rearrangement, T-Lymphocyte; D005865:Gestational Age; D008099:Liver / Q000196:embryology / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009928:Organ Specificity / Q000235:genetics; D016133:Polymerase Chain Reaction; D011948:Receptors, Antigen, T-Cell / Q000096:biosynthesis / Q000235:genetics*; D013950:Thymus Gland / Q000196:embryology* / Q000276:immunology / Q000378:metabolism
chemical_list: D016207:Cytokines; D011948:Receptors, Antigen, T-Cell