The vaccinia virus HindIII F fragment: nucleotide sequence of the left 6.2 kb.
The nucleotide sequence of the left 6.2 kb of the 13.2-kb HindIII F fragment of vaccinia virus was determined. Translation of the sequence revealed nine closely spaced, tandemly oriented open reading frames (ORFs), all reading leftward. The transcriptional organization of this region was determined by Northern blot and S1 nuclease mapping. The analysis suggested that ORFs 1, 2, 4, 5, 6, 7, and 8 are transcribed early in infection, whereas ORFs 3 and 9 are probably late genes. Two of these ORFs have been reported previously. ORF F4L encodes the small subunit of ribonucleotide reductase and ORF F2L is homologous to a retroviral protease-like gene.
Roseman
N A
NA
Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331.
Slabaugh
M B
MB
D000595:Amino Acid Sequence; D001483:Base Sequence; D004279:DNA, Viral / Q000737:chemistry; D015247:Deoxyribonuclease HindIII; D001330:Electronic Data Processing; D005814:Genes, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011544:Pseudogenes; D012333:RNA, Messenger / Q000737:chemistry; D012264:Ribonucleotide Reductases / Q000235:genetics*; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic; D014616:Vaccinia virus / Q000235:genetics*
D004279:DNA, Viral; D012333:RNA, Messenger; D012264:Ribonucleotide Reductases; D015247:Deoxyribonuclease HindIII; D015719:Single-Strand Specific DNA and RNA Endonucleases