Increase of beta 1-6-branched oligosaccharides in human esophageal carcinomas invasive against surrounding tissue in vivo and in vitro.
The -GlcNAc beta 1-6Man- (beta 1-6) branched N-glycosidic oligosaccharides expressed on tumor cells have been found to contribute to malignant and metastatic potential in experimental tumor models. Phaseolus vulgaris leukoagglutinin (L-PHA) requires the beta 1-6-linked lactosamine antenna for high-affinity binding and was used histochemically to characterize the distribution of these sugar structures in human esophageal squamous cell carcinomas from 42 patients. Leukoagglutinin-reactive carcinoma cells in the invasive tumors were distributed predominantly on the outer surface of the tumor adjacent to the surrounding tissue. Furthermore, when TE 1 cells, a human esophageal squamous cell carcinoma line, were cultured in a collagen gel matrix to obtain colonies in a three-dimensional form, these colonies exhibited high affinity for L-PHA binding only in the outer cell layer facing the collagen matrix, unrelated to the cell growth cycle. These findings suggest that the increase in beta 1-6-branched oligosaccharides in esophageal carcinomas is an important trait of the tumor in the invasion into the surrounding tissue.
Takano
R
R
Department of Pathology, Tohoku University School of Medicine, Sendai, Japan.
Nose
M
M
Nishihira
T
T
Kyogoku
M
M
D000117:Acetylglucosamine / Q000032:analysis; D015415:Biomarkers; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D004938:Esophageal Neoplasms / Q000473:pathology*; D006801:Humans; D008358:Mannose / Q000032:analysis; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D009844:Oligosaccharides / Q000032:analysis*; D010835:Phytohemagglutinins; D014407:Tumor Cells, Cultured / Q000166:cytology
D015415:Biomarkers; D009844:Oligosaccharides; D010835:Phytohemagglutinins; C100156:leukoagglutinins, plants; D008358:Mannose; D000117:Acetylglucosamine