Tumors of diverse histology are sensitive to rhodamine 123 laser phototherapy in vitro.
Rhodamine 123 has been shown to be an efficient photosensitizer for the argon laser treatment of a human squamous carcinoma and a melanoma cell line in vitro. Rhodamine 123 laser phototherapy also eradicates these human squamous cell carcinomas when grown as subcutaneous tumor transplants in athymic mice. This study extends these observations by testing a panel of 19 human tumor cell lines of various histologic origins for in vitro sensitivity to rhodamine 123 and the argon laser. Rhodamine 123 with an absorption maxima of 502 nm in water was found to undergo a redshift to 516 nm after uptake by the mitochondria of human tumor cells. Rhodamine 123-sensitized brain tumor cells were inhibited by over 80% after 15 seconds and by 98% after 60 seconds of laser exposure (514.5 nm, 4 W, Tmax = 39 degrees C), as measured by reduced [3H]thymidine incorporation into cellular DNA. Laser or rhodamine 123 alone did not significantly inhibit (greater than 20%) tumor cell [3H]thymidine uptake. Sensitization with 20 micrograms rhodamine 123 for 1 hour before 45 seconds of laser illumination decreased cell [3H]thymidine uptake by 40% to 99% in four melanoma lines, five carcinomas, five leukemias, and four of five other human tumor lines. Two melanomas, two leukemias, and a lymphoma cell line also exhibited a 70% to 80% reduction in [3H]thymidine uptake after sensitization in vitro with 1 microgram/mL rhodamine 123 and laser illumination. Rhodamine 123-sensitized tumor cells were inhibited even more strongly by fractional dose laser irradiation at nonthermal temperatures.(ABSTRACT TRUNCATED AT 250 WORDS)
Sugita
K
K
UCLA Department of Surgery 90024.
Giuliano
A E
AE
Sugita
N
N
Castro
D J
DJ
Fetterman
H R
HR
Ward
P H
PH
Saxton
R E
RE
D002460:Cell Line; D004354:Drug Screening Assays, Antitumor; D005456:Fluorescent Dyes / Q000627:therapeutic use*; D006801:Humans; D066298:In Vitro Techniques; D053685:Laser Therapy; D010778:Photochemotherapy; D020112:Rhodamine 123; D012235:Rhodamines / Q000627:therapeutic use*; D014407:Tumor Cells, Cultured
D005456:Fluorescent Dyes; D012235:Rhodamines; D020112:Rhodamine 123