A study of serum free medium culture on the cloning efficiency of human bone marrow myeloid and erythroid progenitors.
Thirteen different combinations of serum-free media were tested to assess their suitability to replace serum containing medium for in vitro culture of human hemopoietic progenitors (CFU-GM and BFU-E). Bone marrow samples from patients with and without hematological diseases were tested. All tested media supported the growth of CFU-GM and BFU-E colonies, however our results have shown that the cloning efficiency of all commercially available serum-free media tested was lower (mean 18% and 12% of controls for CFU-GM and BFU-E respectively) and the colony size was smaller than those in serum-containing medium. In the serum-free cultures, there was no linear relationship between the colony numbers and cell concentration plated. Depletion of T-lymphocytes and monocytes did not improve the cloning efficiency of the serum-free medium culture. Furthermore, the addition of high concentration of insulin, transferrin and other supplements to the serum-free media did not improve the cloning efficiency. These results have indicated that the currently available commercial serum-free media do not provide optimal requirements for hemopoietic progenitor cell cultures and that other factors contained in serum are essential for their optimal growth.
Wu
Z H
ZH
Department of Hematology, Royal North Shore Hospital, Sydney.
Dowton
L A
LA
Georgiou
G
G
Ma
D D
DD
D001790:Blood Physiological Phenomena; D002478:Cells, Cultured; D002999:Clone Cells; D003470:Culture Media / Q000494:pharmacology*; D015672:Erythroid Precursor Cells / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans
D003470:Culture Media