Endotoxin-induced auto-immunity in mice. III. Comparison of different endotoxin preparations.
Six different endotoxin preparations derived from Escherichia coli and Salmonella typhimurium subspecies were compared as to their potencies to provoke auto-immune phenomena in mice. The numbers of spleen cells forming antibodies to bromelain-treated isologous erythrocytes or anti-DNA antibodies, the serum levels of these auto-antibodies, and the circulating immune complex titres were determined. As far as comparison on a weight base was concerned, S. typhimurium Re-mutant lipopolysaccharide appeared to be the most active preparation in inducing auto-antibody formation. Upon comparison of amounts with equal activity in the limulus amoebocyte lysate assay, however, S. typhimurium lipid A turned out to be the most potent. The contribution of O-type specific polysaccharides, phosphate groups, and the lipid A moiety to the potencies of the endotoxin preparations is discussed.
Bloembergen
P
P
Department of Medical Microbiology, Canisius-Wilhelmina Hospital, Nijmegen, The Netherlands.
Hofhuis
F M
FM
Hol
C
C
van Dijk
H
H
D000818:Animals; D015551:Autoimmunity / Q000187:drug effects* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004731:Endotoxins / Q000008:administration & dosage / Q000494:pharmacology*; D004926:Escherichia coli; D005260:Female; D008033:Limulus Test; D051379:Mice; D008815:Mice, Inbred Strains; D012486:Salmonella typhimurium
D004731:Endotoxins