title: A novel creatine kinase cDNA whose transcript shows enhanced testicular expression.
abstract: A cDNA (TCK1) encoding a creatine kinase (CK) subunit has been isolated from a cDNA library prepared from rainbow trout testis poly(A) + RNA. The predicted amino acid sequence for this CK subunit showed a novel amino-terminal coding region. Northern blot analyses demonstrated preferential expression of this CK subunit in testis relative to other CK containing organs. Southern blot analysis suggested a single copy number for this CK gene.
authors.affiliation: Department of Medical Biochemistry, University of Galgary, Canada.
authors.forename: A T
authors.initials: AT
authors.lastname: Garber
authors.forename: R J
authors.initials: RJ
authors.lastname: Winkfein
authors.forename: G H
authors.initials: GH
authors.lastname: Dixon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003402:Creatine Kinase / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013737:Testis / Q000201:enzymology*; D014158:Transcription, Genetic; D014337:Trout
chemical_list: D003402:Creatine Kinase