Treatment of murine EL4 leukemia in ascitic form with anti-Thy 1.2 specific immunotoxins.
C57BL/6 mice with EL4 leukemia cells in ascitic form were intraperitoneally treated with ricin A chain-multivalent antibody immunotoxins. The immunotoxins containing rabbit IgG anti-Thy 1.2 antibodies complemented by protein A of Staphylococcus aureus were able to interact specifically with the target cells and to induce an antitumor effect as revealed by an increase in survival time of the mice. No apparent secondary effects consecutive to a cytotoxic action on the normal Thy 1.2 antigen bearing cells were observed with the immunotoxin doses used.
Marches
R
R
Department of Immunology, Babes Institute, Bucharest, Romania.
Mota
G
G
Margineanu
M
M
Stavri
H
H
Savi
G
G
Nicolae
M
M
Bancu
A
A
Moraru
I
I
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000627:therapeutic use*; D007167:Immunotherapy; D000922:Immunotoxins / Q000627:therapeutic use*; D007518:Isoantibodies / Q000276:immunology / Q000627:therapeutic use*; D007942:Leukemia, Experimental / Q000628:therapy*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012397:Rosette Formation
D000911:Antibodies, Monoclonal; D000922:Immunotoxins; D007518:Isoantibodies; C035678:anti-Thy antibody