Determination of salicylic acid and its metabolites in urine by derivative synchronous spectrofluorimetry.
The simultaneous determination of salicylic acid in binary and/or ternary mixtures and its two main urinary metabolites is proposed. Mixtures of salicylic, salicyluric and gentisic acids are resolved by synchronous spectrofluorimetry, in combination with first-derivative measurements. The urine is extracted with diethyl ether in acid medium. Salicylic and salicyluric acids are re-extracted into glycine-sodium hydroxide buffer solution of pH 11.6 and determined at that pH, and salicylic and gentisic acids are re-extracted into boric acid-sodium hydroxide buffer solution of pH 8.5 and determined at pH 6.
Salinas
F
F
Department of Analytical Chemistry, University of Extremadura, Badajoz, Spain.
Muñoz de la Peña
A
A
Durán-Merás
I
I
Soledad Durán
M
M
D005841:Gentisates; D006626:Hippurates / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D062385:Hydroxybenzoates / Q000652:urine; D012459:Salicylates / Q000652:urine*; D020156:Salicylic Acid; D013050:Spectrometry, Fluorescence / Q000379:methods*
D005841:Gentisates; D006626:Hippurates; D062385:Hydroxybenzoates; D012459:Salicylates; C000693:salicylurate; D020156:Salicylic Acid; C010925:2,5-dihydroxybenzoic acid