title: The detection of technical failures in perfused heart with ischemia and reperfusion by epicardial NADH fluorescence.
abstract: This study documents the value of continuous observation of nicotinamide adenine dinucleotide (NADH) fluorescence (NADH-F). NADH-F monitoring is used to identify ischemic regions for the recognition of minor technical failures associated with ischemia and reperfusion experiments in the isolated perfused heart system. The visualization of NADH-F is possible by simply irradiating the heart with ultraviolet light. Rat hearts, in the working-heart mode, were subjected to occlusion/reperfusion of the left coronary artery, and analyzed. The perfusate was filtered through a 5 micron pore membrane. Out of 281 hearts which were judged to be free of technical failures by conventional physiological indices (heart rate greater than 200/min, cardiac output greater than 34 ml/min, and coronary flow 9-14 ml/min), 43 (15%) disclosed an abnormal NADH-F area prior to the coronary intervention. During coronary intervention, 29 technical failures were detected as indicated by sparse NADH-F distribution with occlusion, delayed disappearance of NADH-F upon reperfusion, or the exhibition of an abnormal NADH-F region unassociated with the coronary artery supply area. These technical failures are not detectable without the use of NADH-F, although the actual number of failures detected may depend on the skill of the operator. We recommend NADH-F monitoring for any preparations which do not contain hemoglobin, since NADH-F is an intrinsic probe for ischemia and is easily applicable to a variety of experiments.
authors.affiliation: Department of Physiology 2, School of Medicine, Tokai University, Kanagawa, Japan.
authors.forename: K K
authors.initials: KK
authors.lastname: Minezaki
authors.forename: Y
authors.initials: Y
authors.lastname: Shinozaki
authors.forename: H
authors.initials: H
authors.lastname: Nakazawa
authors.forename: Y
authors.initials: Y
authors.lastname: Yamada
authors.forename: H
authors.initials: H
authors.lastname: Okino
mesh_terms: D000818:Animals; D005453:Fluorescence; D008297:Male; D015425:Myocardial Reperfusion; D015428:Myocardial Reperfusion Injury / Q000175:diagnosis*; D009243:NAD / Q000032:analysis*; D010496:Pericardium / Q000737:chemistry*; D010781:Photography; D051381:Rats; D011919:Rats, Inbred Strains; D014466:Ultraviolet Rays
chemical_list: D009243:NAD