Protein differences between normal and oligospermic human sperm demonstrated by two-dimensional gel electrophoresis.
Protein expression by sperm obtained from men with normal semen analysis and men with oligospermia were evaluated by two-dimensional gel electrophoresis. Proteins were solubilized in a 9.5 M urea/2% Nonidet-P40 (LKB, Bromma, Sweden) lysis buffer and underwent second dimension separation on 10 to 16% polyacrylamide gradient gels. A set of 36 invariant proteins was identified in all normospermic samples, whereas 8 of 10 evaluable oligospermic samples lacked 1 or more of the invariant proteins. Proteins absent in oligospermic samples may be critical to normal sperm function and may serve as markers for infertility.
Morgentaler
A
A
Division of Urology, Beth Israel Hospital, Harvard Medical School, Boston, Massachusetts 02115.
Schopperle
W M
WM
Crocker
R H
RH
DeWolf
W C
WC
D015415:Biomarkers; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism; D008297:Male; D009845:Oligospermia / Q000378:metabolism* / Q000503:physiopathology; D011506:Proteins / Q000032:analysis*; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology
D015415:Biomarkers; D011506:Proteins