title: Competitive erythroimmunoassay for detecting Clostridium perfringens type A enterotoxin in stool specimens.
abstract: A competitive erythroimmunoassay (ERIA) is described for Clostridium perfringens enterotoxin (CPE) detection in stools. This technique uses sheep red blood cells sensitized by CPE and an anti-CPE-antibody-coated plate in which the results are read by eye. ERIA is simple, rapid, economic and more sensitive (2 ng/ml) than the enzyme-linked immunosorbent assay used for evaluation. ERIA is suitable for CPE detection in stool samples protected with phenylmethylsulphonylfluoride.
authors.affiliation: Institut Pasteur de Nouméa, Laboratoire des Bactéries entéropathogénes, Nouméa.
authors.forename: Y
authors.initials: Y
authors.lastname: Germani
authors.forename: M R
authors.initials: MR
authors.lastname: Popoff
authors.forename: E
authors.initials: E
authors.lastname: Bégaud
authors.forename: J L
authors.initials: JL
authors.lastname: Guesdon
mesh_terms: D003015:Clostridium Infections / Q000175:diagnosis*; D003016:Clostridium perfringens / Q000302:isolation & purification*; D003967:Diarrhea / Q000382:microbiology*; D004768:Enterotoxins; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007118:Immunoassay; D066298:In Vitro Techniques
chemical_list: D004768:Enterotoxins