Inhibition of TGF alpha-induced second messengers by anti-EGF receptor antibody-425.
Monoclonal antibody 425 binds to a protein epitope of the human EGF receptor and blocks EGF dependent functions such as EGF receptor phosphorylation and mitogenesis (1). We now show that MAb 425 blocks TGF alpha-induced second messenger signals, namely inositol 1,4,5 triphosphate and Ca2+ in two carcinoma cell lines, A 431 and SW-948. In this study we have further characterized the specificity of this antibody for inhibiting TGF alpha induced mitogenesis in MRC-5, a EGF-receptor expressing fibroblast cell line.
Murthy
U
U
Department of Cell Sciences, SmithKline Beecham, King of Prussia, PA 19406.
Rieman
D J
DJ
Rodeck
U
U
D000911:Antibodies, Monoclonal; D002118:Calcium / Q000378:metabolism; D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects*; D066246:ErbB Receptors / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D007700:Kinetics; D015290:Second Messenger Systems / Q000187:drug effects*; D016211:Transforming Growth Factor alpha / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D016211:Transforming Growth Factor alpha; D015544:Inositol 1,4,5-Trisphosphate; D066246:ErbB Receptors; D002118:Calcium