Studies of the rad-equivalence of ethylene oxide in the presence and absence of 12-O-tetradecanoylphorbol-13-acetate (TPA) in C3H/10T1/2 cells.
Cell transformation in vitro of C3H/10T1/2 cells, using gamma-radiation and ethylene oxide (EtO), in both the absence and presence of the cancer promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA), was studied. TPA promotes transformation of C3H/10T1/2 cells to the same extent. In the dose ranges studied the average enhancement of the transformation frequency was 2.4 and 2.5 for EtO and gamma-radiation, respectively. The rad-equivalence of EtO in the presence of TPA was calculated to be 75 +/- 52 rad/mMh (95% confidence interval) which is consistent with the value 78 +/- 14 rad/mMh (95% confidence interval) obtained without TPA treatment.
Kolman
A
A
Department of Radiobiology, Stockholm University, Sweden.
Näslund
M
M
Osterman-Golkar
S
S
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D003470:Culture Media; D005027:Ethylene Oxide / Q000633:toxicity*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D005720:Gamma Rays; D016014:Linear Models; D051379:Mice; D011829:Radiation Dosage; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013997:Time Factors
D003470:Culture Media; D005027:Ethylene Oxide; D013755:Tetradecanoylphorbol Acetate