[Reconstruction of the synthetic gene for human interleukin-2].
Chemical-enzymatic synthesis and cloning of the DNA fragment coding for the human interleukin-2 signal sequence was accomplished. A hybrid plasmid pSIL-2 containing the gene of the human interleukin-2 with this signal sequence was constructed for effective expression of the gene in eukaryotic systems. A variant permitting the removal of the interleukin-2 stop-codons was obtained, which is suitable for the construction of chimeric genes containing the interleukin-2 gene sequence at the 5'-end.
Seregin
S V
SV
Riabinin
V A
VA
Siniakov
A N
AN
Daniliuk
N K
NK
Pozdniakov
S G
SG
D001483:Base Sequence; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D015252:Deoxyribonucleases, Type II Site-Specific; D015870:Gene Expression; D005813:Genes, Synthetic; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010957:Plasmids
D004274:DNA, Recombinant; D007376:Interleukin-2; D015345:Oligonucleotide Probes; C035042:endodeoxyribonuclease FokI; D015252:Deoxyribonucleases, Type II Site-Specific
Rekonstruktsiia iskusstvennogo gena chelovecheskogo interleĭkina-2.