Site specific functionalization of oligonucleotides for attaching two different reporter groups.
The synthesis of an oligonucleotide functionalized to attach two different reporter groups at specific internucleotide linkages is described. To incorporate the amine specific reporter group the internucleotide linkage is modified to phosphoramidate (N-1-aminoalkyl) and for a thiol specific reporter group the internucleotide linkage is modified to a phosphorothioate diester. The synthetic cycle for introducing the modified internucleotide linkages at specific sites can be carried out using an automated DNA synthesizer. Combination of reporter groups have been attached successfully.
Agrawal
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S
Worcester Foundation for Experimental Biology, Shrewsbury, MA 01545.
Zamecnik
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PC
D000577:Amides; D000588:Amines; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D010756:Phosphoric Acids; D013438:Sulfhydryl Compounds
D000577:Amides; D000588:Amines; D009838:Oligodeoxyribonucleotides; D010756:Phosphoric Acids; D013438:Sulfhydryl Compounds; C011067:phosphoramidic acid