Narbonin, a 2 S globulin from Vicia narbonensis L. Crystallization and preliminary crystallographic data.
A seed globulin from Vicia narbonensis L. has been crystallized by vapour diffusion induced pH-shift. Crystals are suitable for high-resolution X-ray structural analysis and diffract to better than 1.5 A. Narbonin crystallizes in the monoclinic space group P21 with alpha = 46.9 A, b = 75.5 A, c = 50.9 A, alpha = gamma = 90 degrees, beta = 120.5 degrees. The protein consists of one polypeptide chain that does not coincide with the subunits of legumin or vicilin after SDS/polyacrylamide gel electrophoresis and has a relative molecular mass of about 33,000.
Hennig
M
M
Institute of Biochemistry, Humboldt University, Berlin, GDR.
Schlesier
B
B
Pfeffer
S
S
Höhne
W E
WE
D003460:Crystallization; D005916:Globulins / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D014674:Plant Proteins, Dietary; D010944:Plants / Q000032:analysis*; D012639:Seeds / Q000032:analysis*; D014961:X-Ray Diffraction
D005916:Globulins; D010940:Plant Proteins; D014674:Plant Proteins, Dietary; C066193:narbonin