The mitochondrial nad5 gene of sugar beet (Beta vulgaris) encoding a subunit of the respiratory NADH dehydrogenase.
We have isolated the nad5 gene from the N-cytoplasm of Beta vulgaris, by heterologous hybridization with a nad5 probe from Chlamydomonas reinhardtii, and have determined the DNA sequence. The gene has a length of 3082 bp and consists of three exons and two introns, 459, 1269, 270, 848 and 357 bp in length, respectively. It has a similarity of 95.1% at the DNA level to the nad5 gene of Oenothera and the two respective proteins show an overall level of amino acid identity of 88.7%. Compared to Oenothera the reading frame of the first exon is extended in the 5' direction and in the third exon there is a frameshift shortening the reading frame by 96 bp and changing the last 48 codons. The nad5 gene is a single copy gene in the N- and S-cytoplasm of Beta vulgaris and the organisation of the gene shows no apparent differences in the two cytoplasms. The gene is actively expressed; three major transcripts are detectable. The transcript patterns have been compared between the two cytoplasms and between different plant tissues.
Ecke
W
W
Botanisches Institut, Universität Düsseldorf, Federal Republic of Germany.
Schmitz
U
U
Michaelis
G
G
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004272:DNA, Mitochondrial / Q000235:genetics*; D005091:Exons; D015870:Gene Expression; D017343:Genes, Plant; D007438:Introns; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D016366:Open Reading Frames; D010944:Plants / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D004272:DNA, Mitochondrial; D009245:NADH Dehydrogenase