High-performance liquid chromatographic approach to the separation of antiviral and immunostimulant fractions in Neuramide.
Neuramide, a tissue extract having antiviral action against influenza A virus and immunostimulant action, was analyzed by preparative size-exclusion high-performance liquid chromatography (HPLC) and a low-molecular-weight fraction responsible for the antiviral action was isolated after reversed-phase HPLC. Four fractions having immunostimulant activity were also isolated, as evidenced by their potentiating action in the human lymphocyte proliferation induced by phytohaemagglutinin.
Miraglia
N
N
Dipartimento di Chimica Organica e Industriale, Università di Milano, Italy.
Rindone
B
B
Folchitto
G
G
Amicucci
P
P
Antonelli
G
G
Lanfranchi
A
A
Massa
M
M
D000276:Adjuvants, Immunologic / Q000302:isolation & purification*; D023181:Antimicrobial Cationic Peptides; D000998:Antiviral Agents / Q000302:isolation & purification*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D066298:In Vitro Techniques; D009980:Influenza A virus / Q000187:drug effects; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects; D008752:Methylene Chloride; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D014462:Ultrafiltration
D000276:Adjuvants, Immunologic; D023181:Antimicrobial Cationic Peptides; D000998:Antiviral Agents; D010455:Peptides; D008752:Methylene Chloride; C039202:neuramide