title: Early accumulation of serotonin in rat spinal cord subjected to traumatic injury. Relation to edema and blood flow changes.
abstract: Changes in the concentration of serotonin (5-hydroxytryptamine) in the early period after a focal traumatic injury to rat spinal cord were determined and related to the formation of edema and alterations in blood flow. A unilateral, 5-mm-long and 3-mm-deep traumatic injury located 2 mm from the midline was created in the T10-11 segment of the cord. Five hours after the injury the serotonin concentration in the traumatized segment had increased more than 100% compared with controls. There was also a progressive increase in water content of the traumatized segment measured 1-5 h after the injury. On the other hand, the spinal cord blood flow showed a progressive decrease to about 35% of its initial value at 5 h. Pretreatment with p-chlorophenylalanine, a serotonin synthesis inhibitor, impeded the elevation in water content measured 5 h after the trauma. The spinal cord blood flow remained close to normal values and the increase in serotonin was absent. Our results show that trauma to the rat spinal cord will induce changes in the serotonin concentration of the tissue and that the associated formation of edema and blood flow alterations can be alleviated in serotonin depleted rats. Obviously, serotonin plays a significant role in the pathophysiology of traumatic injury of rat spinal cord.
authors.affiliation: Institute of Pathology, University Hospital, Uppsala, Sweden.
authors.forename: H S
authors.initials: HS
authors.lastname: Sharma
authors.forename: Y
authors.initials: Y
authors.lastname: Olsson
authors.forename: P K
authors.initials: PK
authors.lastname: Dey
mesh_terms: D000818:Animals; D001834:Body Water / Q000378:metabolism; D004487:Edema / Q000209:etiology*; D005260:Female; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012039:Regional Blood Flow; D012701:Serotonin / Q000097:blood / Q000378:metabolism*; D013116:Spinal Cord / Q000098:blood supply / Q000378:metabolism*; D013119:Spinal Cord Injuries / Q000150:complications / Q000378:metabolism* / Q000503:physiopathology
chemical_list: D012701:Serotonin