Characteristic views of Escherichia coli RNA polymerase core enzyme in the scanning transmission electron microscope.
Eight characteristic views of Escherichia coli RNA polymerase core enzyme are presented which were found using multivariate statistical image processing of about 1000 individual molecular images. The data set was obtained from negatively stained specimens with a scanning transmission electron microscope (STEM) operated in dark field mode. The molecular projections were found to a resolution of around 30 A, thus visualizing new structural details of the core enzyme. An approximate twofold axis seems to relate the beta to the beta' subunit as well as both alpha units to each other.
Tichelaar
W
W
European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
van Heel
M
M
D012321:DNA-Directed RNA Polymerases / Q000145:classification / Q000648:ultrastructure*; D004926:Escherichia coli / Q000201:enzymology*; D008855:Microscopy, Electron, Scanning; D015999:Multivariate Analysis
D012321:DNA-Directed RNA Polymerases