title: Cloning and expression of the carbon monoxide dehydrogenase genes from Pseudomonas thermocarboxydovorans strain C2.
abstract: Carbon monoxide dehydrogenase (CODH) from Pseudomonas thermocarboxydovorans strain C2 is composed of three non-identical subunits. A gene library of C2 DNA in lambda vector L47.1 was generated and screened using anti-CODH serum. Western blotting experiments revealed a protein which co-migrated with and had the same immunological reaction as the large subunit of CODH in some of the clones isolated from the library. The coding region was pinpointed to a 4 kb fragment which was subcloned into plasmid. Western blotting experiments showed that all three subunits of CODH were coded for by the subclone. However, no CODH activity was detected.
authors.affiliation: School of Biology, Sunderland Polytechnic, U.K.
authors.forename: G W
authors.initials: GW
authors.lastname: Black
authors.forename: C M
authors.initials: CM
authors.lastname: Lyons
authors.forename: E
authors.initials: E
authors.lastname: Williams
authors.forename: J
authors.initials: J
authors.lastname: Colby
authors.forename: M
authors.initials: M
authors.lastname: Kehoe
authors.forename: C
authors.initials: C
authors.lastname: O'Reilly
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000096:biosynthesis / Q000235:genetics*; D015153:Blotting, Western; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D015698:Genomic Library; D009097:Multienzyme Complexes; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics*; D015183:Restriction Mapping
chemical_list: D009097:Multienzyme Complexes; D000445:Aldehyde Oxidoreductases; C019823:carbon monoxide dehydrogenase