Vasoactive intestinal peptide stimulation of adenylate cyclase activity in isolated rabbit renal arterial microvessels.
Vasoactive intestinal peptide (VIP) at a concentration of 1 microM stimulates adenylate cyclase (AC) in isolated rabbit renal preglomerular arterial vessels by 3.9 +/- 1.3 fold (n = 6) over basal values. A comparable stimulation of AC was observed with prostaglandin E1. Half-maximal VIP-stimulated activity was observed at 7.2 +/- 3.5 nM (n = 6), which was increased to 17.4 +/- 4.4 nM by 10 microM-(4Cl-D-Phe6,Leu17)VIP, a VIP-receptor antagonist.
Griffiths
N M
NM
Department of Physiological Sciences, Medical School, University of Newcastle upon Tyne.
Simmons
N L
NL
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002196:Capillaries / Q000187:drug effects / Q000378:metabolism*; D005576:Colforsin / Q000494:pharmacology; D005934:Glucagon / Q000494:pharmacology; D066298:In Vitro Techniques; D007668:Kidney / Q000098:blood supply; D008297:Male; D010281:Parathyroid Hormone / Q000494:pharmacology; D011458:Prostaglandins E / Q000494:pharmacology; D011817:Rabbits; D014660:Vasoactive Intestinal Peptide / Q000502:physiology*
D010281:Parathyroid Hormone; D011458:Prostaglandins E; D005576:Colforsin; D014660:Vasoactive Intestinal Peptide; D005934:Glucagon; D000262:Adenylyl Cyclases