ABO blood-typing of bloodstains by dot-ELISA method.
The ABH antigens of bloodstains which were effectively solubilized in phosphate-buffered saline by crushing in a mortar were detected on a nitrocellulose membrane by enzyme-linked immunosorbent assay (ELISA). The analyses for characterization showed that the extracted substances consisted of various components of the red cell with molecular weights of more than 30,000. ABO types of some aged bloodstains were determined by this simple procedure. This method is a useful tool for determining various blood-groups from bloodstain.
Ito
S
S
Department of Legal Medicine, Osaka Medical College, Takatsuki, Japan.
Matsui
K
K
Suzuki
K
K
Matsuo
Y
Y
Matsumoto
H
H
D000017:ABO Blood-Group System; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D001801:Blood Stains; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting
D000017:ABO Blood-Group System