Flumecinol, a novel inducer of testosterone 16 alpha-hydroxylation in male rats.
1. Flumecinol, a new inducer of the cytochrome P-450 monooxygenases, was studied in rats as a possible effector of liver microsomal testosterone oxidases. The drug enhanced the total content of liver cytochrome P-450 in immature and adult rats. 2. When total testosterone oxidation activity was compared in liver microsomes of treated and untreated rats, no differences in activities were observed in 60-day-old-rats, but a slight decrease was found in 35-day-old treated rats. 3. Several regio- and stereo-specific hydroxylases were modified by flumecinol administration; in 35-day-old rats only 16 alpha-hydroxylation was induced, whereas in 60-day-old rats a slight increase in 2 alpha-hydroxylation was also observed.
Silva
M
M
Department of Biochemistry, Faculty of Medicine, University of Chile, Santiago.
Gil
L
L
D000375:Aging / Q000378:metabolism; D000735:Androstenedione / Q000378:metabolism; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D001559:Benzhydryl Compounds / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis* / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D006900:Hydroxylation; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D015394:Molecular Structure; D051381:Rats; D011919:Rats, Inbred Strains; D039181:Steroid 16-alpha-Hydroxylase; D013250:Steroid Hydroxylases / Q000096:biosynthesis*; D013739:Testosterone / Q000378:metabolism*
D001559:Benzhydryl Compounds; D013739:Testosterone; D000735:Androstenedione; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; D039181:Steroid 16-alpha-Hydroxylase; C022724:testosterone 7-alpha-hydroxylase, hamster; C016914:flumecinol