title: [Development of ELISPOT assay for thyroid autoantibody-producing cells].
abstract: A new assay system for detection of thyroid-autoantibody-producing cells was developed. This assay is based on the ELISPOT method with antigen-coated nitrocellulose membranes in 96-well microfilter plates. This was more sensitive than conventional methods such as a radioimmunoassay and a passive agglutination method for detection of thyroid-autoantibody production. The coefficients of inter- and intra-assay variations for antibody-producing cells were less than 6.5%. Thus, this assay system can be used to analyse the thyroid-specific immunological abnormalities as a routine test.
authors.affiliation: Department of Laboratory Medicine, Osaka University School of Medicine.
authors.forename: T
authors.initials: T
authors.lastname: Kaneda
authors.forename: Y
authors.initials: Y
authors.lastname: Iwatani
authors.forename: N
authors.initials: N
authors.lastname: Amino
authors.forename: H
authors.initials: H
authors.lastname: Tamaki
authors.forename: Y
authors.initials: Y
authors.lastname: Hidaka
authors.forename: E
authors.initials: E
authors.lastname: Suehisa
authors.forename: H
authors.initials: H
authors.lastname: Hosotsubo
authors.forename: J
authors.initials: J
authors.lastname: Hayashi
authors.forename: K
authors.initials: K
authors.lastname: Miyai
mesh_terms: D000372:Agglutination Tests; D000921:Antibody-Producing Cells / Q000166:cytology*; D001323:Autoantibodies / Q000096:biosynthesis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000276:immunology; D011863:Radioimmunoassay; D013961:Thyroid Gland / Q000276:immunology*
chemical_list: D001323:Autoantibodies