[Effects of methylmercury on ethanol-induced sleeping time of mice].
This study was carried out to clarify the effects of methylmercury intoxication on the ethanol-induced sleeping time of mice. The mice were injected with methylmercury chloride (MMC) (10 mgMMC/kg body weight) subcutaneously for 1, 5 and 10 successive days (1, 5 and 10 inj.), and control mice received only saline. Twenty-four hours, after the last injection, these mice were treated with ethanol (4.5 mgEtOH/kg body weight) intraperitoneally and subsequent sleeping time was observed. After 24 hours, mice were sacrificed to measure the concentration of MMC in various brain regions and liver. A similar experiment with ethanol treatment was also performed to assay the biogenic monoamines in various brain regions and alcohol dehydrogenase (ADH) activity in liver. The results can be summarized as follows: 1) Ethanol-induced sleeping time was 170 min. in the 10 inj. and it was significantly longer than saline, 1 and 5 inj. However, other experimental groups showed no change when compared with saline. 2) Norepinephrine levels increased in white matter and pons + medulla after 1 inj. 3) Dopamine levels increased only in white matter of the 10 inj. when compared with saline. No changes were shown in the other groups. 4) Serotonin levels increased in all the regions after 1 inj. 5) ADH activity in liver did not show any alteration during the experimental period when compared with saline.(ABSTRACT TRUNCATED AT 250 WORDS)
Sano
K
K
Institute of Community Medicine, University of Tsukuba, Ibaraki.
Shimojo
N
N
Yamaguchi
S
S
D000818:Animals; D001679:Biogenic Amines / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D008297:Male; D008767:Methylmercury Compounds / Q000633:toxicity*; D051379:Mice; D008813:Mice, Inbred ICR; D012890:Sleep / Q000187:drug effects*; D013997:Time Factors
D001679:Biogenic Amines; D008767:Methylmercury Compounds; D000431:Ethanol