Novel beta-lactamase from Capnocytophaga sp.
A novel beta-lactamase activity which confers resistance to expanded-spectrum cephalosporins and penicillins has been found in strain IC 5/21 of Capnocytophaga spp. Enzyme activity migrated at a molecular size of 38,000 daltons and at an isoelectric point of 3.6, with a minor band at 4.1. Kinetic studies suggested that it belonged to Richmond and Sykes beta-lactamase class 1c. Isoelectric focusing could be achieved only if a nonionic detergent was added to the gel, suggesting the presence of a hydrophobic enzyme akin to a membrane-bound beta-lactamase of gram-positive bacteria. The location of the gene coding for this beta-lactamase is not yet known.
Foweraker
J E
JE
Department of Microbiology, University of Leeds, United Kingdom.
Hawkey
P M
PM
Heritage
J
J
Van Landuyt
H W
HW
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002206:Capnocytophaga / Q000201:enzymology*; D004352:Drug Resistance, Microbial; D007202:Indicators and Reagents; D007525:Isoelectric Focusing; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D010957:Plasmids; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D007202:Indicators and Reagents; D047090:beta-Lactams; D001618:beta-Lactamases