Inhibition of myosin light chain phosphorylation in cultured smooth muscle cells by HA1077, a new type of vasodilator.
When cultured smooth muscle cells were stimulated sequentially by concanavalin A and fetal calf serum, the cells rounded up, and there was an accompanying mono- and diphosphorylation of the 20 kDa myosin light chain. HA1077, a new type of vasodilator, inhibited both the cell rounding and the light chain phosphorylation in a concentration dependent manner. Since HA1077 inhibits myosin light chain kinase, in vitro, we propose that this vasodilator presumably inhibits cell rounding by limiting myosin light chain phosphorylation.
Sasaki
Y
Y
Biochemical Research Lab., Asahi Chemical Industry, Co., Ltd., Miyazaki, Japan.
Sasaki
Y
Y
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000031:analogs & derivatives*; D000818:Animals; D001011:Aorta / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D007546:Isoquinolines / Q000494:pharmacology*; D007700:Kinetics; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015879:Myosin Subfragments / Q000378:metabolism*; D010766:Phosphorylation; D011817:Rabbits; D014665:Vasodilator Agents / Q000494:pharmacology*
D007546:Isoquinolines; D015879:Myosin Subfragments; D014665:Vasodilator Agents; D003208:Concanavalin A; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C049347:fasudil