title: Coupling of Na+ with the spermidine transporter protein in NIH BALB/c 3T3 cells.
abstract: This study demonstrates that polyamine spermidine (Spd) transporter protein is directly coupled with the Na+ in a ternary complex form, Na(+)-Spd-carrier. The Spd is transported with Na+ in a 1:1 stoichiometry relationship. Interestingly, addition of 2-deoxyglucose in the assay medium did not influence significantly the Spd uptake demonstrating the ATP independency of Spd transport.
authors.affiliation: Dept. of Cell Biology, Central Hospital University (C.H.U.) of Rennes I, Faculty of Medicine, France.
authors.forename: N A
authors.initials: NA
authors.lastname: Khan
authors.forename: I
authors.initials: I
authors.lastname: Masson
authors.forename: V
authors.initials: V
authors.lastname: Quemener
authors.forename: J P
authors.initials: JP
authors.lastname: Moulinoux
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D051379:Mice; D008807:Mice, Inbred BALB C; D012964:Sodium / Q000378:metabolism*; D013095:Spermidine / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D000255:Adenosine Triphosphate; D012964:Sodium; D013095:Spermidine