title: Borate G-banding technique applicable to in situ deoxyribonucleic acid/deoxyribonucleic acid hybridized chicken chromosomes.
abstract: The borate G-banding technique produced excellent longitudinal bands similar to trypsin G-bands on chicken chromosomes prepared from embryo material. Aged chromosome preparations on slides were pretreated in borate buffer (pH 9.2) for 10 to 30 s at 37 C, then stained in Giemsa solution. This simple G-banding procedure gave high-resolution chromosome banding and permitted the visualization of silver grains that localized cloned gene sequences or cloned DNA fragments to specific regions by in situ hybridization.
authors.affiliation: Department of Animal Science, University of Minnesota, St. Paul 55108.
authors.forename: E M
authors.initials: EM
authors.lastname: Shaw
authors.forename: J S
authors.initials: JS
authors.lastname: Otis
authors.forename: K S
authors.initials: KS
authors.lastname: Guise
authors.forename: R N
authors.initials: RN
authors.lastname: Shoffner
mesh_terms: D000818:Animals; D001881:Borates; D002645:Chickens / Q000097:blood / Q000235:genetics*; D002871:Chromosome Banding / Q000379:methods*; D002875:Chromosomes; D004247:DNA / Q000032:analysis; D007962:Leukocytes / Q000648:ultrastructure; D008677:Metaphase; D009693:Nucleic Acid Hybridization
chemical_list: D001881:Borates; D004247:DNA