title: [The incorporation of macromolecules into the germ cells of male mice via electroporation and dimethyl sulfoxide].
abstract: Electroporation (incorporation of macromolecules into the living cells by means of electric pulses) provides inclusion of plasmid 14C-DNA into immature cells of spermatogenic epithelium. The highest level of foreign DNA incorporation into spermatocytes and spermatids has been induced by 8kV electric pulses applied 3 times with 20 sec intervals. Meanwhile, mature sperms are found to be exclusively resistant to exogenous DNA irrespective of the voltage level, the number of pulses and Ca++ uptake (contents). Incubation of mature sperms for two hours in the medium with Ca++ (10 mM) and dimethylsulfoxide--(DMSO, 33%) provides highly reliable incorporation of plasmid 14C-DNA into sperm heads. The sperm cells with foreign DNA incorporated by means of Ca++ and DMSO treatment still remain alive and mobile. The possibilities of mature sperms loaded with foreign DNA for the creation of transgenic mammals are discussed.
authors.forename: V S
authors.initials: VS
authors.lastname: Baranov
authors.forename: I
authors.initials: I
authors.lastname: Hapala
authors.forename: P
authors.initials: P
authors.lastname: Hrijac
authors.forename: L
authors.initials: L
authors.lastname: Kovac
authors.forename: K
authors.initials: K
authors.lastname: Boda
mesh_terms: D000818:Animals; D001345:Autoradiography; D002250:Carbon Radioisotopes; D004271:DNA, Fungal / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004560:Electricity; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D046911:Macromolecular Substances; D008297:Male; D008722:Methods; D051379:Mice; D008808:Mice, Inbred CBA; D010957:Plasmids; D013091:Spermatogenesis / Q000187:drug effects / Q000502:physiology; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism*
chemical_list: D002250:Carbon Radioisotopes; D004271:DNA, Fungal; D046911:Macromolecular Substances; D004121:Dimethyl Sulfoxide
vernacular_title: Vkliuchenie makromolekul v polovye kletki samtsov mysheĭ pri pomoshchi élektroporatsii i dimetilsul'foksida.