title: Resistance to apramycin in two enterobacterial clinical isolates: detection of a 3-N-acetyltransferase IV.
abstract: Considering the possible role of farm animals in the contamination of human consumers by plasmid-mediated apramycin-resistant enterobacteria strains, this type of resistance should be tested more systematically in human isolates. Very recently we isolated in Zaragoza one apramycin-resistant Escheria coli strain obtained from the blood of a hospitalized patient; this clinical isolate produced a plasmid-mediated 3-N-aminoglycoside acetyltransferase IV. We describe also the isolation in Madrid of one multiresistant Klebsiella pneumoniae clinical strain. This isolate harbored a single plasmid and carried determinants for apramycin, gentamicin, tobramycin, hygromycin B, streptomycin, and ampicillin, which could be transferred en bloc to E. coli K-12 J62. Extracts from donor and transconjugant strains carrying pUZ6776 plasmid produce acetyltransferase activity AAC(3)-IV and double phosphotransferase activity (HPH and APH(3'')).
authors.affiliation: Department of Microbiology, Faculty of Medicine, University of Zaragoza, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Gómez-Lus
authors.forename: M J
authors.initials: MJ
authors.lastname: Rivera
authors.forename: M L
authors.initials: ML
authors.lastname: Gómez-Lus
authors.forename: J
authors.initials: J
authors.lastname: Gil
authors.forename: S
authors.initials: S
authors.lastname: Gómez-Lus
authors.forename: J
authors.initials: J
authors.lastname: Castillo
authors.forename: P
authors.initials: P
authors.lastname: Goñi
authors.forename: P
authors.initials: P
authors.lastname: Madero
authors.forename: M C
authors.initials: MC
authors.lastname: Rubio
mesh_terms: D000123:Acetyltransferases / Q000378:metabolism*; D004352:Drug Resistance, Microbial; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D009328:Nebramycin / Q000031:analogs & derivatives* / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics; D013045:Species Specificity
chemical_list: D009328:Nebramycin; C011666:apramycin; D000123:Acetyltransferases; C045539:aminoglycoside N(3')-acetyltransferase