Isolation and identification of anaerobic bacteria from ovine foot rot in Spain.
A microbiological study was made of 125 Merino sheep showing clinical signs of foot rot. A total of 435 strictly anaerobic strains were isolated, belonging to the following genera: Bacteroides, Peptostreptococcus, Tissierella, Fusobacterium, Megasphaera, Eubacterium, Acidaminococcus, Clostridium, Peptococcus and Propionibacterium. Of the 35 species obtained, the following were found in more than 10 per cent of animals sampled: Bacteroides nodosus, B putredinis, B buccae, B ruminicola subspecies brevis, Tissierella praeacuta, Peptostreptococcus anaerobius and Megasphaera elsdenii. Six culture media were used for isolation. Agar brucella and agar brucella enriched with G-N anaerobe supplement proved to be the most efficient for isolating anaerobic bacteria.
Piriz Duran
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S
Unidad de Microbiología e Inmunología, Facultad de Veterinaria, Universidad de Extremadura, Carretera de Trujillo, Cáceres, Spain.
Cuenca Valera
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Valle Manzano
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J
Vadillo Machota
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D000818:Animals; D001421:Bacteria, Anaerobic / Q000302:isolation & purification*; D003470:Culture Media; D005535:Foot Rot / Q000382:microbiology*; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*; D013030:Spain
D003470:Culture Media