title: Determination of salicylic acid and its metabolites in urine by derivative synchronous spectrofluorimetry.
abstract: The simultaneous determination of salicylic acid in binary and/or ternary mixtures and its two main urinary metabolites is proposed. Mixtures of salicylic, salicyluric and gentisic acids are resolved by synchronous spectrofluorimetry, in combination with first-derivative measurements. The urine is extracted with diethyl ether in acid medium. Salicylic and salicyluric acids are re-extracted into glycine-sodium hydroxide buffer solution of pH 11.6 and determined at that pH, and salicylic and gentisic acids are re-extracted into boric acid-sodium hydroxide buffer solution of pH 8.5 and determined at pH 6.
authors.affiliation: Department of Analytical Chemistry, University of Extremadura, Badajoz, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Salinas
authors.forename: A
authors.initials: A
authors.lastname: Muñoz de la Peña
authors.forename: I
authors.initials: I
authors.lastname: Durán-Merás
authors.forename: M
authors.initials: M
authors.lastname: Soledad Durán
mesh_terms: D005841:Gentisates; D006626:Hippurates / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D062385:Hydroxybenzoates / Q000652:urine; D012459:Salicylates / Q000652:urine*; D020156:Salicylic Acid; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005841:Gentisates; D006626:Hippurates; D062385:Hydroxybenzoates; D012459:Salicylates; C000693:salicylurate; D020156:Salicylic Acid; C010925:2,5-dihydroxybenzoic acid