title: Effects of microdialysis on brain metabolism in normal and seizure states.
abstract: The effect of intracranial microdialysis on brain glucose metabolism in control and kainic acid-treated rats was assessed by semi-quantitative [14C]2-deoxyglucose autoradiography. A dialysis fiber loop was implanted into the piriform cortex or a horizontal Vita fiber into the hippocampus, and 24 h later, fibers were perfused with Krebs-Ringer bicarbonate solution before and after injection of kainic acid (16 mg/kg, i.p.) [14C]2-Deoxyglucose was injected i.p. 3 h after the injection of kainic acid. Rats injected with kainic acid were initially lethargic and then proceeded through behavioral phases of staring, "wet-dog shakes", Straub tail, rearing, forepaw clonus, and, in some cases, tonic-clonic convulsions. Three hours after kainic acid, the fiber presence in the piriform cortex enhanced kainic acid-induced metabolic activity in areas adjacent to the fiber assembly, whereas the fiber in hippocampus attenuated kainic acid-induced metabolic activity in areas adjacent to the fiber assembly. The results indicate that intracranial microdialysis alters the already abnormal brain metabolism in a kainic acid-induced seizure state, but has no significant effect in the non-seizure control state.
authors.affiliation: Department of Pharmacology, University of Kansas Medical Center, College of Health Sciences and Hospital, Kansas City 66103.
authors.forename: J E
authors.initials: JE
authors.lastname: Chastain
authors.forename: F
authors.initials: F
authors.lastname: Samson
authors.forename: S R
authors.initials: SR
authors.lastname: Nelson
authors.forename: T L
authors.initials: TL
authors.lastname: Pazdernik
mesh_terms: D000818:Animals; D001345:Autoradiography; D001522:Behavior, Animal / Q000502:physiology; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism*; D003847:Deoxyglucose / Q000494:pharmacology; D003956:Dialysis; D005947:Glucose / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012640:Seizures / Q000378:metabolism* / Q000503:physiopathology; D013238:Stereotaxic Techniques
chemical_list: D003847:Deoxyglucose; D005947:Glucose