Potassium inhibition of DMH-induced small intestinal tumors in rats.
This is a preliminary report that shows that supplemental potassium partially prevents 1,2-dimethylhydrazine (DMH) induction of tumors of the small intestine in Sprague-Dawley rats. Male rats were injected weekly with 20 mg DMH/kg body wt for 20 weeks. Potassium chloride was provided in the drinking water one week before the first DMH treatment and was continued until sacrifice 14 weeks after the last DMH treatment. There were four groups of rats and they were identified as follows: DMH, DMH + K, K, and untreated control. Based on 24-hour food and water consumption data and food and water compositions, rats provided 0.5% potassium (from KCl in the drinking water) were ingesting 287.5 +/- 9.2 mg potassium per 24 hours (K/Na = 4.18) and the unsupplemented groups were ingesting 180.3 +/- 18.4 mg potassium per 24 hours (K/Na = 2.62). At sacrifice, the incidence of DMH-induced small intestinal tumors was significantly (p less than 0.05) reduced from 46% (6/13) in the DMH group to 6% (1/17) in the DMH + K group (p less than 0.05). The potassium supplement also significantly (p less than 0.05) reduced the cumulative small intestinal tumor incidence from 40% (8/20) in the DMH group to 5% (1/20) in the DMH + K group. The incidence of colon tumors and of zymbal gland tumors appeared to be reduced by the potassium supplement; however, these were not statistically significant observations (p greater than 0.05). Based on the complete blood count and other blood parameters measured, the level of potassium supplement used induced no apparent toxic effects.(ABSTRACT TRUNCATED AT 250 WORDS)
Jacobs
M M
MM
American Institute for Cancer Research, Washington, DC 20009.
D019813:1,2-Dimethylhydrazine; D000818:Animals; D004127:Dimethylhydrazines; D004326:Drinking; D005982:Glutathione Transferase / Q000378:metabolism; D006403:Hematologic Tests; D007414:Intestinal Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D008099:Liver / Q000201:enzymology; D008297:Male; D011189:Potassium Chloride / Q000008:administration & dosage* / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D004127:Dimethylhydrazines; D011189:Potassium Chloride; D005982:Glutathione Transferase; D019813:1,2-Dimethylhydrazine