Affinity immunoblotting detection of serum monoclonal immunoglobulins reactive with glycosphingolipids.
The characterization of serum monoclonal IgM reactive with glycosphingolipid present in the nervous system is of major importance in patients with certain neuropathies. A rapid, sensitive and specific one-step method which permits such a characterization is described. Serum immunoglobulins separated by high resolution agarose electrophoresis are transferred to affinity filters (nitrocellulose sheets coated with glycolipids), and revealed with enzyme-tagged monospecific anti-immunoglobulin antibodies.
Jauberteau
M O
MO
Laboratory of Immunology, University Hospital, Limoges, France.
Cook
J M
JM
Drouet
M
M
Preud'homme
J L
JL
D000911:Antibodies, Monoclonal / Q000097:blood* / Q000276:immunology; D001927:Brain Diseases / Q000097:blood*; D003711:Demyelinating Diseases / Q000276:immunology; D004587:Electrophoresis, Agar Gel; D006028:Glycosphingolipids / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D007075:Immunoglobulin M / Q000032:analysis* / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D006028:Glycosphingolipids; D007075:Immunoglobulin M