15-Lipoxygenase products affect protein phosphorylation in Friend erythroleukemia cells.
Endogenous arachidonic acid metabolism and protein phosphorylation have been examined in Friend erythroleukemia cells in response to the induction of differentiation by dimethyl sulfoxide and hexamethylene bisacetamide. 15-Hydroxyeicosatetraenoic acid levels were elevated in cells differentiated with hexamethylene bisacetamide or dimethyl sulfoxide compared with undifferentiated cells. Protein phosphorylation decreased markedly in differentiated cells compared with undifferentiated cells and the addition of 15-hydroperoxyeicosatetraenoic acid specifically decreased the phosphorylation of a 28-kilodalton protein. These findings indicate that products of 15-lipoxygenase may act as intracellular messengers in Friend erythroleukemia cells by affecting protein phosphorylation.
Beckman
B S
BS
Department of Pharmacology, Tulane University School of Medicine, New Orleans, LA 70112.
Jeter
J R
JR
D000818:Animals; D001093:Arachidonate 15-Lipoxygenase / Q000378:metabolism*; D002460:Cell Line; D005622:Friend murine leukemia virus; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D015289:Leukotrienes / Q000494:pharmacology*; D008054:Lipid Peroxides / Q000494:pharmacology*; D051379:Mice; D009363:Neoplasm Proteins / Q000187:drug effects / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects
D015289:Leukotrienes; D008054:Lipid Peroxides; D009363:Neoplasm Proteins; C025086:15-hydroperoxy-5,8,11,13-eicosatetraenoic acid; D001093:Arachidonate 15-Lipoxygenase