Activation of idiotype-specific CD4+ T-cell line: cellular processing of exogenous self-immunoglobulin.
An idiotype (Id)-specific long-term cultured T-cell line has been generated from BALB/c mice immunized with M315 (alpha, lambda 2). The cell line comprises both CD3+ and CD4+ but CD8- cells. The T-cell line is stimulated in a class II major histocompatibility complex (MHC)-restricted manner, and is capable of producing interleukin-2 (IL-2) in response to the Id along with Iad-bearing antigen-presenting cells (APC). Fine Id specificity analysis has shown that changes in amino acid residues, Phe-94, Arg-95 and Asn-96, located on the VL-315, resulting from a somatic mutation mechanism of the mouse V lambda 2 gene, contribute to the T-cell activation. Pretreatment of APC with either glutaraldehyde or paraformaldehyde prevented both Fv-315 and VL-315 from triggering the T cells. This suggested that further processing of VL is required for T-cell activation. To clarify this point, we have generated a synthetic peptide, designated P18, which spans residues 91-108 of VL-315. In sharp contrast to VL, prefixed APC were capable of presenting P18 to stimulate the T-cell line to induce IL-2.
Sakato
N
N
Department of Immunochemistry, Osaka University, Japan.
Rugdech
P
P
Yoda
T
T
Ota
A
A
Zhao
Y
Y
Semma
M
M
Suzuki
Y
Y
Fujio
H
H
D000818:Animals; D000918:Antibody Specificity / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000276:immunology; D015704:CD4 Antigens / Q000032:analysis*; D002460:Cell Line; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D015704:CD4 Antigens; D007130:Immunoglobulin Idiotypes; D010446:Peptide Fragments