[The effect of cryopreservation on the cytogenetic characteristics of a cell subline of skin fibroblasts from the Indian muntjac].
After thawing cells, previously cryopreserved in the presence of dimethyl sulfoxide (DMSO), a decrease in their viability and increase in unscheduled DNA synthesis was observed. In 7 days, these parameters restored to the control level. Cryopreservation without DMSO resulted in the decrease in both cell viability and replicative and unscheduled DNA synthesis. In 14 days, these characteristics were seen to return to the normal level. Cryopreservation of cells without DMSO and their preservation in liquid nitrogen induced the frequency of chromosomal aberrations, mostly chromosomal breaks. The frequency of chromosomal aberrations increased with the duration of cell preservation in liquid nitrogen. The normal level was achieved following 7 days after cell thawing. Cells treated with DMSO only (without cryopreservation) display an increased number of chromosomal and chromatid breaks and translocations. Nonrandom distribution of chromosomal aberrations was observed, with particular chromosomes being involved in the appearance of dicentrics and translocations. The data obtained indicate that cryoprotective activity of DMSO is probably associated with the cell repair systems. The detected antimutagenic and mutagenic activity of DMSO may presumably reflect various conditions for its interaction with cells (with or without cryopreservation), as well as it may be specific for the muntjac cell line used in the present work.
Polianskaia
G G
GG
Semenova
E G
EG
Shubin
N A
NA
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D002869:Chromosome Aberrations; D015925:Cryopreservation / Q000379:methods*; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D003670:Deer / Q000235:genetics*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D008677:Metaphase / Q000187:drug effects; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D013997:Time Factors
D004247:DNA; D004121:Dimethyl Sulfoxide
Vliianie kriokonservatsii na tsitogeneticheskie kharakteristiki kletochnoĭ sublinii fibroblastov kozhi indiĭskogo muntzhaka.