Cresyl violet: a red fluorescent Nissl stain.
Cresyl violet is widely used by neurobiologists to visualize Nissl substance in bright-field microscopy. Here we describe a method for using this dye as a red fluorescent Nissl stain. Unlike the bright-field staining technique, fluorescent cresyl is compatible with other fluorescent dyes and tracers, such as fluorescein, Fluoro-Gold and Fast Blue. The procedure requires only minor modifications of routine bright-field cresyl staining, the most significant being dilution of the stain. Thus, fluorescent red cresyl violet is simple to implement and may be of general use in fluorescence microscopy.
Alvarez-Buylla
A
A
Rockefeller University, New York, NY 10021.
Ling
C Y
CY
Kirn
J R
JR
D000818:Animals; D048588:Benzoxazines; D001717:Birds; D005456:Fluorescent Dyes; D009562:Nissl Bodies / Q000648:ultrastructure*; D010078:Oxazines; D014466:Ultraviolet Rays
D048588:Benzoxazines; D005456:Fluorescent Dyes; D010078:Oxazines; C028911:cresyl violet