Direct sectioning of unembedded cartilage: a simple method for microscopical and histochemical studies on chondrocytes and extracellular matrix.
On account of the rigidity and compact structure of the hyaline cartilage, unfixed or formaldehyde fixed samples of this tissue can be directly sectioned by using a conventional ultramicrotome and a glass knife. This simple method allows to obtain microscopical sections from unembedded cartilage blocks, which show a well preserved histological structure and are very suitable to carry out morphological and histochemical studies on chondrocytes and cartilaginous matrix.
Stockert
J C
JC
Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
Del Castillo
P
P
D000818:Animals; D002356:Cartilage / Q000033:anatomy & histology* / Q000737:chemistry / Q000166:cytology; D005109:Extracellular Matrix / Q000737:chemistry / Q000648:ultrastructure; D006651:Histocytochemistry; D008867:Microtomy / Q000379:methods*