Initial steps in reverse cholesterol transport: the role of short-lived cholesterol acceptors.
The early metabolism of cell-derived cholesterol was followed during the interaction of normolipaemic native plasma with cultured cell monolayers labelled to high specific activity with 3H-cholesterol. Kinetic analysis indicated that initial processing involved several prebeta-migrating high-density lipoprotein (HDL) species. A small prebeta species, the initial acceptor, was first converted to a lipoprotein whose composition predicts a discoidal structure. The free cholesterol content of this was then esterified by lecithin: cholesterol acyltransferase, and its cholesterol converted to the alpha mobility of spheroidal mature HDL the binding of apolipoprotein A-II. These studies follow the genesis of HDL in plasma from peripheral cell membrane cholesterol.
Francone
O L
OL
Cardiovascular Research Institute, University of California Medical Centre, San Francisco.
Fielding
C J
CJ
D001692:Biological Transport / Q000502:physiology; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008075:Lipoproteins, HDL / Q000737:chemistry; D008970:Molecular Weight
D008075:Lipoproteins, HDL; D002784:Cholesterol