Strain specific variation of outer membrane proteins of wild Yersinia pestis strains subjected to different growth temperatures.
Three Yersinia pestis strains isolated from humans and one laboratory strain (EV76) were grown in rich media at 28 degrees C and 37 degrees C and their outer membrane protein composition compared by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Several proteins with molecular weights ranging from 34 kDa to 71 kDa were observed to change in relative abundance in samples grown at different temperatures. At least seven Y. pestis outer membrane proteins showed a temperature-dependent and strain-specific behaviour. Some differences between the outer membrane proteins of full-pathogenic wild isolates and the EV76 strain could also be detected and the relevance of this finding on the use of laboratory strains as a reference to the study of Y. pestis biological properties is discussed.
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Centro de Pesquisas Aggeu Magalhães, FIOCRUZ, Recife, PE, Brasil.
de Almeida
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Ferreira
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D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D006801:Humans; D013696:Temperature; D015010:Yersinia pestis / Q000032:analysis* / Q000254:growth & development / Q000302:isolation & purification
D001425:Bacterial Outer Membrane Proteins