High-performance liquid chromatography patterns of mycolic acids as criteria for identification of Mycobacterium chelonae, Mycobacterium fortuitum, and Mycobacterium smegmatis.
Rapidly growing mycobacteria of clinical significance were identified by mycolic acids detected with high-performance liquid chromatography. Mycolic acids from whole cells were extracted, derivatized, and detected by a modified high-performance liquid chromatography procedure in less than 3 h. Use of an internal standard allowed differentiation of Mycobacterium chelonae and Mycobacterium fortuitum by comparison of relative retention times. Peak height ratios were used for subidentification of M. chelonae strains; however, M. fortuitum and Mycobacterium smegmatis could not be separated by this system.
Butler
W R
WR
Division of Bacterial Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
Kilburn
J O
JO
D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D006801:Humans; D009161:Mycobacterium / Q000032:analysis* / Q000145:classification / Q000302:isolation & purification; D009171:Mycolic Acids / Q000302:isolation & purification* / Q000592:standards; D009170:Nontuberculous Mycobacteria / Q000032:analysis / Q000145:classification / Q000302:isolation & purification; D012015:Reference Standards; D013045:Species Specificity; D013696:Temperature
D003470:Culture Media; D009171:Mycolic Acids