title: Effect of phorbol myristate acetate on processing of formyl peptide receptors by human neutrophils.
abstract: We examined the effect of phorbol myristate acetate on the ability of human neutrophils to process formyl peptide receptors. The receptor was affinity-labeled and its extracellular localization assessed over time, by cleavage of extracellular labeled receptor with papain. Neutrophils were capable of internalizing (and/or recycling) affinity labeled formyl peptide receptor in the absence of extracellular calcium. This phenomenon was dependent upon stimulation with phorbol myristate acetate, suggesting a role for protein kinase C in this process.
authors.affiliation: Rosalind Russell Arthritis Research Laboratories, University of California, San Francisco.
authors.forename: E
authors.initials: E
authors.lastname: Lobo
authors.forename: F
authors.initials: F
authors.lastname: Elfman
authors.forename: E
authors.initials: E
authors.lastname: Kelly
authors.forename: H D
authors.initials: HD
authors.lastname: Perez
mesh_terms: D001667:Binding, Competitive; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000097:blood*; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
chemical_list: D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic; D009240:N-Formylmethionine Leucyl-Phenylalanine; D013755:Tetradecanoylphorbol Acetate