Application of sodium dodecyl sulfate-gel electrophoresis to low molecular weight polypeptides.
Experiments with nine polypeptides with molecular weights between 2000 and 10,760 confirm the value of sodium dodecy sulfate (SDS)-gel electrophoresis for separating polypeptides in this molecular weight range. In one case, electrophoretic blotting and microsequencing were successfully carried out. However, molecular weight determination in the low molecular weight range (less than 10,000) is much less reliable than that in the conventional molecular weight range (greater than 10,000) for SDS gels. Information provided by suppliers of horse heart myoglobin fragment kits is potentially misleading.
Huang
J M
JM
Department of Biological Chemistry, University of California, Davis 95616.
Matthews
H R
HR
D000818:Animals; D002417:Cattle; D004563:Electrochemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000032:analysis; D006736:Horses; D006868:Hydrolysis; D007328:Insulin / Q000032:analysis; D008970:Molecular Weight; D009206:Myocardium / Q000737:chemistry; D009211:Myoglobin / Q000032:analysis; D010455:Peptides / Q000032:analysis*
D006657:Histones; D007328:Insulin; D009211:Myoglobin; D010455:Peptides