Preimplantation development in the streptozotocin-induced diabetic mouse.
Streptozotocin (STZ) was used to develop a diabetic mouse model in which to study the development of the preimplantation embryo. STZ doses of 0, 160, 190, 210 and 240 mg kg-1 were given; 190 mg kg-1 was found to be the most suitable as the standard diabetogenic dose, providing about 60% mice with plasma glucose greater than 20 mM. The STZ-diabetic mice responded to superovulation with 10 i.u. of gonadotrophin in the same manner as control mice, producing similar embryo numbers at 48 h, 72 h and 96 h post-hCG. Furthermore, the proportion of 2-cell embryos collected from STZ-diabetic mice which developed to blastocysts in vitro was similar to that of 2-cell embryos from control mice. The STZ-diabetic mouse model after superovulation thus produced normal early preimplantation embryos whose development can be examined in detail in a diabetic environment.
Beebe
L F
LF
Department of Physiology and Pharmacology, University of Queensland, Australia.
Kaye
P L
PL
D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D005260:Female; D051379:Mice; D011247:Pregnancy; D011254:Pregnancy in Diabetics / Q000503:physiopathology*