Sequence similarities between tryptophan synthase beta subunit and other pyridoxal-phosphate-dependent enzymes.
On the basis of 8 tryptophan synthase beta subunits (EC 4.2.1.20) consensus patterns were constructed comprising two conserved motifs. Screening of the SWISSPROT protein sequence database with these patterns indicates similarities with O-acetylserine sulfhydrolases (EC 4.2.99.8), threonine synthases (EC 4.2.99.2), L- and D-serine dehydratases (EC 4.2.1.13/EC 4.2.1.14) and threonine dehydratases (EC 4.2.1.16). Using multiple alignment procedures the similar regions could be extended. In connection with their pyridoxal-phosphate-binding-capacity and their positions in biochemical pathways evolutionary relationships among these enzymes are discussed.
Bork
P
P
Academy of Sciences of the GDR, Central Institute of Molecular Biology, Department of Biomathematics, Berlin.
Rohde
K
K
D000595:Amino Acid Sequence; D008190:Lyases / Q000737:chemistry / Q000235:genetics*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D011732:Pyridoxal Phosphate / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D014367:Tryptophan Synthase / Q000737:chemistry / Q000235:genetics*
D046911:Macromolecular Substances; D011732:Pyridoxal Phosphate; D008190:Lyases; D014367:Tryptophan Synthase