The cAMP-dependent protein kinase in sea urchin sperm tails: association of the enzyme with the flagellar axonemes.
When sea urchin spermatozoa were treated with a Triton X-100 solution, cAMP-dependent protein kinase (cA-kinase) activity was extracted. Further extraction with Triton X-100 of axonemes isolated from the Triton-extracted sperm again released a considerable amount of the cA-kinase activity. The activity which remained after extraction three times with Triton X-100 was released by treatment with a low salt solution. These activities found in the various extracts were likely to be due to the same cA-kinase, which was a mammalian type II-like enzyme. The cA-kinase activity that remained in the axonemes after the first Triton X-100 extraction may be involved in the regulation of flagellar movement in the Triton-extracted sperm.
Yokota
E
E
Department of Biology, College of Arts and Sciences, University of Tokyo.
Mabuchi
I
I
D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D008970:Molecular Weight; D017830:Octoxynol; D011092:Polyethylene Glycols; D011494:Protein Kinases / Q000302:isolation & purification / Q000378:metabolism*; D012617:Sea Urchins / Q000201:enzymology*; D013081:Sperm Motility / Q000187:drug effects; D013082:Sperm Tail / Q000201:enzymology*
D011092:Polyethylene Glycols; D017830:Octoxynol; D011494:Protein Kinases