The use of a multichannel scaling method to detect radioactive species in spread monolayers.
This study describes a simple and inexpensive method for monitoring radioactive species spread as monolayers at the air/water interface. The combination of a discriminator and multichannel scalar counter with a personal computer functions to unify all measurements, to simplify the operational process and data acquisition, and to provide a real-time display of the data. Its use is demonstrated by following the hydrolysis of L-alpha-[1-14C]dioleoyl phosphatidylcholine by the enzyme, phospholipase A2, isolated from the porcine pancreas.
Tsao
F H
FH
Department of Pediatrics, University of Wisconsin, Madison 53706.
Foo
T K
TK
DeLuca
P M
PM
Zografi
G
G
D000818:Animals; D002250:Carbon Radioisotopes / Q000032:analysis; D006868:Hydrolysis; D066298:In Vitro Techniques; D008567:Membranes, Artificial; D010179:Pancreas / Q000201:enzymology; D010713:Phosphatidylcholines / Q000032:analysis; D010741:Phospholipases A / Q000032:analysis; D054467:Phospholipases A2; D011868:Radioisotopes / Q000032:analysis*; D013552:Swine
D002250:Carbon Radioisotopes; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D011868:Radioisotopes; D010741:Phospholipases A; D054467:Phospholipases A2; C017251:1,2-oleoylphosphatidylcholine