title: Growth, terpenoid production and antibacterial activity of an in vitro culture of the liverwort Fossombronia pusilla.
abstract: In vitro cultures of the liverwort, Fossombronia pusilla, have been ititiated from spores. They grew well on Gamborg B5 medium supplemented with 2% saccharose and 12.5 micrograms/l vitamin B12. Undifferentiated cultures were obtained on Knop mineral solution with 4% glucose. The cultures produced the same constituents as collected material. Perrottetianal A and B, known substances from liverworts were isolated and characterized by spectroscopic methods as well as alpha-(-)-santonin. A hitherto unknown diterpene dialdehyde-8-hydroxy-9-hydroperrottetianal A--could also be characterized. Furthermore, 7 terpenes were identified by GC/MS. A petrol ether extract and the isolated terpenes exerted antibacterial activity.
authors.affiliation: Pharmakognosie und Analytische Phytochemie, Universität des Saarlandes, Federal Republic of Germany.
authors.forename: M
authors.initials: M
authors.lastname: Sauerwein
authors.forename: H
authors.initials: H
authors.lastname: Becker
mesh_terms: D000890:Anti-Infective Agents; D063245:Plant Development; D010936:Plant Extracts / Q000494:pharmacology*; D010944:Plants / Q000378:metabolism; D013057:Spectrum Analysis; D013729:Terpenes / Q000378:metabolism*
chemical_list: D000890:Anti-Infective Agents; D010936:Plant Extracts; D013729:Terpenes