[Development of ELISPOT assay for thyroid autoantibody-producing cells].
A new assay system for detection of thyroid-autoantibody-producing cells was developed. This assay is based on the ELISPOT method with antigen-coated nitrocellulose membranes in 96-well microfilter plates. This was more sensitive than conventional methods such as a radioimmunoassay and a passive agglutination method for detection of thyroid-autoantibody production. The coefficients of inter- and intra-assay variations for antibody-producing cells were less than 6.5%. Thus, this assay system can be used to analyse the thyroid-specific immunological abnormalities as a routine test.
Kaneda
T
T
Department of Laboratory Medicine, Osaka University School of Medicine.
Iwatani
Y
Y
Amino
N
N
Tamaki
H
H
Hidaka
Y
Y
Suehisa
E
E
Hosotsubo
H
H
Hayashi
J
J
Miyai
K
K
D000372:Agglutination Tests; D000921:Antibody-Producing Cells / Q000166:cytology*; D001323:Autoantibodies / Q000096:biosynthesis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000276:immunology; D011863:Radioimmunoassay; D013961:Thyroid Gland / Q000276:immunology*
D001323:Autoantibodies