Early changes in the rat pancreatic B cell size induced by glucose.
The perimeter, cell area and volume density (Vvi) of B cells and exocytotic images present in these cells were measured in rat pancreas perfused with 3.3 or 16.6 mM glucose. Four minutes after the beginning of 16.6-mM glucose perfusion and coincident with the appearance at the apex of the first phase of insulin secretion, all these parameters underwent a significant increase. The changes observed in the perimeter, the cell area and the Vvi of B cells suggest an increase in their surface area. An imbalance in the rate of endocytosis:exocytosis processes with a relative predominance of the latter would increase the length of the plasma membrane and could be responsible, at least partly, for the changes in the B cell size.
Semino
M C
MC
CENEXA-Centro de Endocrinología Experimental y Aplicada (UNLP-CONICET), Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Argentina.
Gagliardino
A M
AM
Bianchi
C
C
Rebolledo
O R
OR
Gagliardino
J J
JJ
D000818:Animals; D005089:Exocytosis / Q000187:drug effects; D005260:Female; D005947:Glucose / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D005947:Glucose