Effect of Melothria maderaspatana on carbon tetrachloride-induced changes in rat hepatic microsomal drug-metabolizing enzyme activity.
Treatment with an aqueous extract of the aerial parts of Melothria maderaspatana, before or after CCl4 administration in rats markedly decreased CCl4-mediated reductions in aniline hydroxylase and p-aminopyrine N-demethylase activities. Phenobarbital-induced sleeping time in rats and kinetic enzyme studies showed that the effect of the plant was neither due to an induction of the drug metabolizing enzymes nor due to an alteration in the Km values of the enzymes.
Jayatilaka
K A
KA
Department of Biochemistry, Faculty of Medicine, University of Ruhuna, Galle, Sri Lanka.
Thabrew
M I
MI
Perera
D J
DJ
D000633:Aminopyrine N-Demethylase / Q000378:metabolism; D000815:Aniline Hydroxylase / Q000378:metabolism; D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology; D005978:Glutathione / Q000097:blood / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D010634:Phenobarbital / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012890:Sleep / Q000187:drug effects
D004364:Pharmaceutical Preparations; D010936:Plant Extracts; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; D006899:Mixed Function Oxygenases; D000815:Aniline Hydroxylase; D000633:Aminopyrine N-Demethylase; D005978:Glutathione; D010634:Phenobarbital