title: Spatial arrangements of microfibrils in myocardial scars: application of antibody to fibrillin.
abstract: Acute myocardial infarction kills myocytes; viable and necrotic myocytes disconnect and the ends of the viable cells become anchored to collagen fibers during reparative scar tissue formation. These anchorages have not been examined in detail, although previous studies have shown that microfibrils (MFs) concentrate at the edges of scars and at the tips of normal papillary muscles. We examined the spatial arrangements of MFs at these two sites in human hearts. Light and electron microscopic observations were made on tissue samples oriented in the long axis of myocytes and stained with monoclonal antibodies to fibrillin, a glycoprotein component of human microfibrils. MFs had identical arrangements at both sites, where they formed fibrous connections between myofibers and collagen fibers. These connections were oriented in the long axis of the muscle cells. At the myocyte ends, MFs appeared to intertwine with MFs in the normal endomysium; in the main body of the connections, MFs formed compact, 200 to 500 nm thick, fibrillin-positive fibers; and at the collagen ends, MFs splayed out among collagen fibrils. These observations indicate that MFs form myofiber-collagen fiber linkages at sites where the power of myocardial contraction is being transmitted to the extracellular connective tissue framework. Formation of such linkages seems to be an important step in the successful repair of necrotic myocardial lesions.
authors.affiliation: Laboratory Service, Veterans Affairs Medical Center, Seattle, Washington 98108.
authors.forename: R
authors.initials: R
authors.lastname: Vracko
authors.forename: D
authors.initials: D
authors.lastname: Thorning
authors.forename: R G
authors.initials: RG
authors.lastname: Frederickson
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D002921:Cicatrix / Q000473:pathology*; D003238:Connective Tissue / Q000648:ultrastructure; D005260:Female; D000071837:Fibrillins; D006801:Humans; D007150:Immunohistochemistry; D007365:Intercellular Junctions / Q000648:ultrastructure; D008297:Male; D008840:Microfilament Proteins / Q000276:immunology / Q000378:metabolism; D008854:Microscopy, Electron; D008875:Middle Aged; D009203:Myocardial Infarction / Q000378:metabolism / Q000473:pathology; D009206:Myocardium / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D009210:Myofibrils / Q000648:ultrastructure*; D010210:Papillary Muscles / Q000648:ultrastructure
chemical_list: D000911:Antibodies, Monoclonal; D000071837:Fibrillins; D008840:Microfilament Proteins