Effects of microdialysis on brain metabolism in normal and seizure states.
The effect of intracranial microdialysis on brain glucose metabolism in control and kainic acid-treated rats was assessed by semi-quantitative [14C]2-deoxyglucose autoradiography. A dialysis fiber loop was implanted into the piriform cortex or a horizontal Vita fiber into the hippocampus, and 24 h later, fibers were perfused with Krebs-Ringer bicarbonate solution before and after injection of kainic acid (16 mg/kg, i.p.) [14C]2-Deoxyglucose was injected i.p. 3 h after the injection of kainic acid. Rats injected with kainic acid were initially lethargic and then proceeded through behavioral phases of staring, "wet-dog shakes", Straub tail, rearing, forepaw clonus, and, in some cases, tonic-clonic convulsions. Three hours after kainic acid, the fiber presence in the piriform cortex enhanced kainic acid-induced metabolic activity in areas adjacent to the fiber assembly, whereas the fiber in hippocampus attenuated kainic acid-induced metabolic activity in areas adjacent to the fiber assembly. The results indicate that intracranial microdialysis alters the already abnormal brain metabolism in a kainic acid-induced seizure state, but has no significant effect in the non-seizure control state.
Chastain
J E
JE
Department of Pharmacology, University of Kansas Medical Center, College of Health Sciences and Hospital, Kansas City 66103.
Samson
F
F
Nelson
S R
SR
Pazdernik
T L
TL
D000818:Animals; D001345:Autoradiography; D001522:Behavior, Animal / Q000502:physiology; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism*; D003847:Deoxyglucose / Q000494:pharmacology; D003956:Dialysis; D005947:Glucose / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012640:Seizures / Q000378:metabolism* / Q000503:physiopathology; D013238:Stereotaxic Techniques
D003847:Deoxyglucose; D005947:Glucose