Antibody-dependent enhancement of SIV infection: further characterization and cross reactivity between macaque and sooty mangabey isolates.
Plasma from four rhesus macaques (Macaca mulatta), of which two were experimentally infected with the simian immunodeficiency virus (SIV) isolate SIVmac251, one with isolate SIVsmF236, and another with a SIVsmF236 molecular clone, SIVsmH-4, enhanced SIVmac infection of MT-2 cells. In addition to SIV-positive plasma, infection-enhancement required complement, CD4, and CR2. Titers of infection-enhancing antibodies appeared to correlate with disease progression. The MT-2/SIVmac251 system should be useful in future studies of complement-mediated, antibody-dependent enhancement of macaque and sooty mangabey SIV isolates.
Montefiori
D C
DC
Department of Pathology, Vanderbilt University, School of Medicine, Nashville, TN.
Robinson
W E
WE
Hirsch
V M
VM
Modliszewski
A
A
Mitchell
W M
WM
Johnson
P R
PR
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D015704:CD4 Antigens / Q000276:immunology; D002520:Cercopithecidae; D003167:Complement Activation; D003165:Complement System Proteins / Q000276:immunology*; D003429:Cross Reactions; D008253:Macaca mulatta; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology*; D015302:Simian Immunodeficiency Virus / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015704:CD4 Antigens; D003165:Complement System Proteins