title: [Use of the ELISA immunoenzyme test and indirect immunofluorescence in the diagnosis of a toxoplasmosis epidemic].
abstract: Etiologic agent of lymphadenitis outbreak in one our military environment was detected by two serologic tests: ELISA (enzyme-linked immunosorbent assay) and indirect immunofluorescence test (IIF) both specific for IgM and IgG antibody detection in toxoplasmosis diagnosis. IgM-ELISA analysis of the first serum sample taken from a group of 79 soldiers with lymphadenitis was positive in 65 examinees (82.28%) and IgG-IIF technique in 52 (65.82%). In a group of 33 soldiers from the same unit with no signs of the disease IgM-ELISA test was positive in 12 (36.36%) and IgM-IIF in 9 (27.27%). In a group of 40 officers from the unit with the disease outbreak specific IgM antibodies were found by both tests only in the head cook. IgM antibodies were not found in a control group of 102 healthy soldiers from other units of the same garrison. Results of this investigation thermal injury showed variations in immune response to infection in dependence on trauma effect duration (in dependence on the phase). In the early posttraumatic phase organism is capable to react effectively to the inoculated infective agent and in the late posttraumatic phase this reaction is much less effective. Authors conclude that these results may influence upon the thermal injury management strategy in dependence on the trauma effect duration.
authors.affiliation: Zavod za preventivnu medicinu, Vojnomedicinska akademija.
authors.forename: S
authors.initials: S
authors.lastname: Radulović
authors.forename: L
authors.initials: L
authors.lastname: Videnović
authors.forename: B
authors.initials: B
authors.lastname: Joković
authors.forename: D
authors.initials: D
authors.lastname: Dordević
authors.forename: R
authors.initials: R
authors.lastname: Lalić
authors.forename: D
authors.initials: D
authors.lastname: Pokorni
authors.forename: P
authors.initials: P
authors.lastname: Mijusković
authors.forename: M
authors.initials: M
authors.lastname: Cirković
mesh_terms: D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D008889:Military Personnel; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000453:epidemiology; D015022:Yugoslavia / Q000453:epidemiology
vernacular_title: Primena imunoenzimskog testa ELISA i indirektne imunoflurescencije u dijagnozi epidemije toksoplazmoze.