Enzymic synthesis of useful chito-oligosaccharides utilizing transglycosylation by chitinolytic enzymes in a buffer containing ammonium sulfate.
A chitinase purified from culture filtrates of Trichoderma resei KDR-11 efficiently catalyzed a transglycosylation reaction on tetra-N-acetylchitotetraoside in a buffer medium containing ammonium sulfate, converting the tetrasaccharide into hexa-N-acetylchitohexaose (39.6%) and di-N-acetylchitobiose (55.7%) as the major products. Sugar-chain elongation from di-N-acetylchitobiose as the initial substrate to hexa-N-acetyl-chitohexaose and hepta-N-acetylchitoheptaose was also efficiently induced through lysozyme catalysis in the presence of ammonium sulfate at high (30%) concentration. In this case, the addition of ammonium sulfate to the reaction system resulted in a remarkable increase of the hexamer and heptamer productions, which are desirable as biologically active oligosaccharides.
Usui
T
T
Department of Agricultural Chemistry, Faculty of Agriculture, Shizuoka University, Japan.
Matsui
H
H
Isobe
K
K
D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D000645:Ammonium Sulfate; D000970:Antineoplastic Agents / Q000378:metabolism*; D002021:Buffers; D002688:Chitinases / Q000302:isolation & purification / Q000378:metabolism*; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004187:Disaccharides; D004795:Enzyme Stability; D005936:Glucans / Q000096:biosynthesis*; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000494:pharmacology; D009113:Muramidase / Q000378:metabolism; D009844:Oligosaccharides / Q000096:biosynthesis*; D013696:Temperature; D014242:Trichoderma / Q000201:enzymology
D000970:Antineoplastic Agents; D002021:Buffers; D004187:Disaccharides; D005936:Glucans; D008670:Metals; D009844:Oligosaccharides; C010913:N,N-diacetylchitobiose; C056529:N-acetylchitohexaose; C066261:hepta-N-acetylchitoheptaose; D002688:Chitinases; D009113:Muramidase; D000645:Ammonium Sulfate; D000117:Acetylglucosamine