Integration host factor affects expression of two genes at the conjugal transfer origin of plasmid R100.
Integration host factor (IHF) binds to two sites near the origin of transfer of the conjugative antibiotic resistance plasmid, R100. DNase I footprinting shows that one site is immediately adjacent to oriT and the gene X promoter, and another is adjacent to the traM promoter. A third site, known only from retardation gels, is near the traJ promoter. The relative promoter activities of genes X, traJ and traM are reduced in himA mutants (IHF-), as measured by chloramphenicol-resistance assays. Transcript analyses by Northern blots showed a reduction in size of the principal gene X and traJ transcripts in the absence of IHF.
Dempsey
W B
WB
Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas.
Fee
B E
BE
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002702:Chloramphenicol Resistance; D002874:Chromosome Mapping; D003227:Conjugation, Genetic; D004268:DNA-Binding Proteins / Q000235:genetics*; D003850:Deoxyribonuclease I; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D034982:Integration Host Factors; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D034982:Integration Host Factors; D003850:Deoxyribonuclease I