Inhibition of proliferation of HeLa cells by cocultivation with early mouse embryos (preliminary report).
Eight- to 16-cell zona-free mouse embryos were cocultivated with 5 to 10 HeLa cells in cultivation drops of 0.1 microliter containing D-PBS medium supplemented with 20% fetal bovine serum. The control group included HeLa cells alone cultivated in the same medium under the same culture conditions. The two cultures were evaluated after 72 hours. The control group showed 147 out of 181 cases of proliferation of HeLa cells (81.22%), whereas HeLa cells cocultivated with zona-free mouse embryos only proliferated in 2 out of 156 cases (1.28%). Proliferation of HeLa cells was inhibited even though these were not in direct contact with the embryos. The experiments suggest that the secretory activity of early embryos in culture medium inhibits proliferation of transformed cells via a special agent.
Malec
V
V
Department of Radiotherapy, District Hospital, Trencín.
Babusík
P
P
Kulísková
L
L
D000818:Animals; D002455:Cell Division; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D004622:Embryo, Mammalian / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D051379:Mice