Heat-induced preferential synthesis and redistribution of HSP 70 and 28 families in Chinese hamster ovary cells.
We observed that members of two HSP families (70 and 28 kDa) preferentially redistributed into the nucleus after heating at 45.5 degrees C for 10 min. The rates of synthesis and redistribution of these proteins were different for each member of HSP families during incubation period at 37 degrees C after heat shock. The maximum rates of synthesis of HSP 70 and HSP 28 families, except HSP 28c, were 6-9 hr after heat shock, whereas the maximum rates of redistribution were 3-6 hr after heat shock. These results suggest that the rates of redistribution of these proteins may be dependent on the amount of intracellular proteins as well as the alteration of binding affinity of nucleoproteins following heat shock.
Lee
Y J
YJ
Department of Radiation Oncology, William Beaumont Hospital, Royal Oak, Michigan 48073.
Curetty
L
L
Corry
P M
PM
D000818:Animals; D001345:Autoradiography; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D006358:Hot Temperature; D007700:Kinetics; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D010053:Ovary; D013997:Time Factors; D014316:Tritium
D006360:Heat-Shock Proteins; D014316:Tritium; D008715:Methionine