Quartz-induced production of reactive oxygen metabolites by activated human monocyte-derived macrophages.
Quartz but not titanium dioxide (TiO2) induced the production of reactive oxygen metabolites (ROM) by human monocyte-derived macrophages, as measured by lucigenin dependent chemiluminescence. Activation of the macrophages with BCG, bacterial lipopolysaccharide and macrophage-activating factor (MAF) caused a prominent increase of quartz-induced ROM production, MAF having the strongest effect. The activation did not affect the TiO2 responses to the same extent. Assuming that ROM have a role in the pathogenesis of silica-induced disease in man, we suggest that enhancement of quartz-induced production of ROM by activated pulmonary macrophages may at least partly explain the experimental and epidemiological data indicating that activation of the immune system during infection promotes the development of silicosis.
Nyberg
P
P
Institute of Occupational Health, Helsinki, Finland.
Klockars
M
M
D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation; D016215:Macrophage-Activating Factors / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D009000:Monocytes / Q000276:immunology; D010100:Oxygen / Q000378:metabolism*; D011791:Quartz / Q000633:toxicity*; D012829:Silicosis / Q000209:etiology; D014025:Titanium / Q000633:toxicity
D008070:Lipopolysaccharides; D016215:Macrophage-Activating Factors; D011791:Quartz; C009495:titanium dioxide; D014025:Titanium; D010100:Oxygen