Characterization of the tumorigenic and metastatic potential of a poorly differentiated human colon cancer cell line.
We characterized the tumorigenic and metastatic potential of a poorly differentiated, non-CEA-producing colon cancer cell line, MIP-101, after injection at different sites in athymic mice. After subcutaneous and intrasplenic injection tumor grew locally in 100 and 50%, respectively, but no metastases were found, even after intravenous injection. Intraperitoneal implantation, however, resulted in a high tumor take (10/10) and subsequent liver colonization (8/10 mice). Exogenous CEA prior to intrasplenic injection induced metastasis in 7/8 mice (in 2 mice to the liver and in 5 mice to the lung). Intrasplenic injection of CX-1, a good CEA producer, resulted in hepatic metastases in 100% of the animals. These data suggest a direct or indirect role of CEA in the metastatic process. We conclude that MIP-101 has a high tumorigenic and invasive potential but a low metastatic proclivity, except when grown in the peritoneum, and that pretreatment of tumor-bearing animals with CEA affects the metastatic proclivity.
Wagner
H E
HE
Department of Surgery, New England Deaconess Hospital, Harvard Medical School, Boston, Mass.
Thomas
P
P
Wolf
B C
BC
Zamcheck
N
N
Jessup
J M
JM
Steele
G D
GD
D000230:Adenocarcinoma / Q000473:pathology*; D000818:Animals; D002272:Carcinoembryonic Antigen / Q000097:blood / Q000493:pharmacokinetics / Q000494:pharmacology; D003110:Colonic Neoplasms / Q000473:pathology*; D006801:Humans; D007275:Injections, Intravenous; D008114:Liver Neoplasms, Experimental / Q000556:secondary; D051379:Mice; D008819:Mice, Nude / Q000378:metabolism; D009362:Neoplasm Metastasis / Q000473:pathology*; D009368:Neoplasm Transplantation; D010534:Peritoneal Neoplasms / Q000473:pathology; D013154:Spleen / Q000473:pathology; D014407:Tumor Cells, Cultured
D002272:Carcinoembryonic Antigen