A transforming Kirsten ras oncogene in an oral squamous carcinoma.
This investigation has employed the NIH 3T3 cell transfection assay in an effort to detect transforming genes in DNA from squamous carcinomas of the head and neck. Of 11 tumor DNAs tested, 1 DNA sample from a gingival squamous carcinoma was able to produce primary and secondary transformants containing the human K-ras oncogene. This is the first report of an activated ras oncogene derived from a carcinoma of the head and neck. Head and neck cancers may possess activated ras oncogenes more often than is indicated by this study because of the relative inefficiency of transfection assays in detecting large transforming genes such as K-ras.
Howell
R E
RE
Department of Oral Biology, Dalhousie University, Halifax, Nova Scotia, Canada.
Wong
F S
FS
Fenwick
R G
RG
D000818:Animals; D015139:Blotting, Southern; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics; D004586:Electrophoresis; D011905:Genes, ras / Q000502:physiology*; D006801:Humans; D015151:Immunoblotting; D008297:Male; D051379:Mice; D009062:Mouth Neoplasms / Q000235:genetics*; D009154:Mutation; D009693:Nucleic Acid Hybridization
D015342:DNA Probes; D004273:DNA, Neoplasm