Numbers of steroidogenic luteal cells in Booroola Merino ewes.
In Exp. 1 ovulation rates, plasma concentrations of progesterone, mean individual and total CL weights were determined on Days 4, 10 and 12 after oestrus of Booroola Merino ++ ewes and FF ewes. Mean ovulation rates ranged from 1.5 to 1.8 in ++ ewes and from 5.3 to 6.2 in FF ewes (P less than 0.01). There were no differences in plasma concentrations of progesterone or total luteal weight between the two groups on any of the days studied. Individual CL were smaller (P less than 0.01) in FF ewes than in ++ ewes. In Exp. 2 the numbers of luteal cells in CL collected from 5 ++ and 5 FF ewes on Day 10 of the oestrous cycle were morphometrically determined. The CL from FF ewes were smaller (P less than 0.01) and had fewer total steroidogenic cells (P less than 0.01), fibroblasts (P less than 0.01), and capillary endothelial cells and pericytes (P less than 0.05). However, the luteal cell volume density, number of cells/g tissue, average cell diameter or average cell volume was not different between the two groups of ewes for any cell type studied. It is concluded that the 5-6 CL in FF ewes function in an identical fashion to the 1-2 CL in ++ ewes.
Niswender
G D
GD
Department of Physiology, Colorado State University, Fort Collins 80523.
McNatty
K P
KP
Smith
P
P
Niswender
K D
KD
Farin
C E
CE
Sawyer
H R
HR
D000818:Animals; D003338:Corpus Luteum / Q000033:anatomy & histology / Q000166:cytology / Q000378:metabolism*; D004971:Estrus / Q000097:blood; D005260:Female; D010060:Ovulation / Q000502:physiology; D011374:Progesterone / Q000096:biosynthesis* / Q000097:blood; D012756:Sheep / Q000378:metabolism*
D011374:Progesterone