Resistance to pactamycin in clones of Streptomyces lividans containing DNA from pactamycin-producing Streptomyces pactum.
A pactamycin (Pc)-resistance determinant (pct) from Streptomyces pactum has been isolated on a 4.9-kb KpnI fragment. The original construct involving plasmid pIJ702 was highly unstable in Streptomyces lividans, leading to deletion of the pct gene from the vector. Subcloning of pct into an alternative vector (pOJ160) led to the generation of a more stable clone which possessed Pc-resistant ribosomes, and reconstitution analysis established that 16S rRNA was responsible for such resistance. Post-transcriptional modification of rRNA is probably the mechanism of resistance since the cloned DNA fragment did not appear to encode 16S rRNA.
Calcutt
M J
MJ
Department of Biochemistry, University of Leicester, U.K.
Cundliffe
E
E
D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial; D009693:Nucleic Acid Hybridization; D010142:Pactamycin; D012323:RNA Processing, Post-Transcriptional; D012336:RNA, Ribosomal, 16S / Q000502:physiology*; D015183:Restriction Mapping; D012270:Ribosomes / Q000502:physiology* / Q000648:ultrastructure; D013302:Streptomyces / Q000235:genetics*
D012336:RNA, Ribosomal, 16S; D010142:Pactamycin