The thresholds of the jaw-opening reflex and trigeminal brainstem neurons to tooth-pulp stimulation in acutely and chronically prepared cats.
Electrical stimuli were applied to tooth-pulp in cats and the thresholds of the jaw-opening reflex and of neurons in the trigeminal sensory nuclei were determined. The effects of the method of preparation of the animal for stereotaxic recording were determined by making observations on animals set up in one of three ways: acutely in the usual manner; chronically, three to five days before recording; and acutely with precautions to minimize nociceptive input to the central nervous system. The threshold of the jaw-opening reflex increased progressively during the setting up of the normal, acute preparations and at the time brainstem recording began was significantly higher in these than in either the chronic or low-trauma acute preparations. Previous studies have shown that the increase in threshold is maintained for several hours and is not due to the effects of the anaesthetic. In normal acute preparations, few units (27/154) were found that had thresholds below 50 microA, 0.1 ms, whereas many units were encountered that responded to such a stimulus in chronic (147/152) and low-trauma acute (99/127) animals. In the chronic and in low-trauma acute preparations, there was no significant difference between the thresholds of the units in the main sensory trigeminal nucleus and spinal subnucleus oralis compared with those in subnucleus caudalis. Thus the preparation of an animal for stereotaxic recording can cause a severe and long-lasting depression in the excitability of neurons in the trigeminal sensory nuclei and an increase in the threshold of the jaw-opening reflex. This effect will have influenced the results of previous studies on the responses evoked in central neurons by stimulation of tooth-pulp, and may have similarly affected recordings from other regions.
Clarke
R W
RW
Department of Physiology, University of Bristol, U.K.
Matthews
B
B
D000758:Anesthesia; D000818:Animals; D001930:Brain Injuries / Q000503:physiopathology; D001933:Brain Stem / Q000166:cytology / Q000502:physiology*; D002415:Cats; D003782:Dental Pulp / Q000502:physiology*; D004558:Electric Stimulation; D004566:Electrodes; D004576:Electromyography; D005260:Female; D007568:Jaw / Q000294:innervation*; D008297:Male; D009474:Neurons / Q000502:physiology*; D010146:Pain / Q000503:physiopathology; D012018:Reflex / Q000502:physiology*; D012684:Sensory Thresholds / Q000502:physiology*; D013238:Stereotaxic Techniques; D014276:Trigeminal Nerve / Q000166:cytology / Q000502:physiology*