[Intensive growth of human melanoma cells in a fibrin clot implanted in immunosuppressed mice].
BRO human melanoma cells encapsulated within a fibrin clot grew rapidly in mice immunosuppressed (IS) by wholebody irradiation (5.5 or 6.5 Gy). After 8 days growth under the sub-renal capsule (SRC) of IS mice, the tumor volume increased 40-fold on the average, and histological and cytological examination showed that the growth consisted almost entirely of viable tumor cells. Marked tumor growth in IS mice also was observed after intraperitoneal implantation of fibrin clots containing BRO cells. SRC implantation of encapsulated BRO cells in pre-irradiated mice offers a practical and reproducible method for evaluating the growth of human tumors in vivo.
Lokshin
A
A
Polianskaia
N I
NI
Mashkovtsev
Iu V
IuV
Merkulova
I V
IV
Sokolova
V K
VK
Gavrilova
T N
TN
D000818:Animals; D005337:Fibrin; D006801:Humans; D007165:Immunosuppression Therapy; D008546:Melanoma, Experimental / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011829:Radiation Dosage; D013370:Subrenal Capsule Assay; D014407:Tumor Cells, Cultured; D014916:Whole-Body Irradiation
D005337:Fibrin
Intensivnyĭ rost kletok melanomy cheloveka, implantirovannykh immunodepressirovannym mysham v fibrinovom sgustke.