Analysis of T-2 toxin metabolites in tissues and excreta of rats.
In previous journal articles, we have demonstrated that microsomal carboxyesterase deacetylated specifically the C-4 acetyl residue of T-2 toxin and the resulting HT-2 toxin was an end product of the in vitro system. In an attempt to confirm this evidence in the in vivo system, we have analyzed the metabolism of T-2 toxin in rats. Male rats received orally 2-5 mg/kg of T-2 toxin, and the contents of T-2 toxin, HT-2 toxin, and neosolaniol in the liver, kidney, and serum were analyzed by using GLC method. The data revealed that HT-2 toxin was found in the liver shortly after the administration, and neither T-2 toxin nor neosolaniol was detected in the tissues. Blue spot test on TLC by using 4-(p-nitrobenzyl)pyridine revealed the presence of HT-2 toxin and T-2 tetraol in the urine and HT-2 toxin in the feces.
Ueno
Y
Y
Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
Takai
Y
Y
Baba
Y
Y
D000818:Animals; D002849:Chromatography, Gas; D005243:Feces / Q000737:chemistry*; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013605:T-2 Toxin / Q000378:metabolism*; D014018:Tissue Distribution
D013605:T-2 Toxin