title: Evidence for a feedback regulated back-up promoter which controls permanent expression of a Dictyostelium gene.
abstract: We have previously described the elements of two overlapping promoters regulating differential expression of the Dictyostelium P8A7 gene. One promoter was constitutively active but stimulated during development (S-promoter), while the other (L-promoter) was found to be induced by a variety of stimuli. This effect was mediated by the induction (i-) element. By a series of antisense transformation experiments we here provide evidence that this element is the target of a feedback control circuit. In wild type cells, feedback regulation activates the L-promoter in unfavourable environmental situations when transcription from the S-promoter is insufficient to generate adequate amounts of protein. A model for the function of the feedback loop under natural and experimental conditions is presented.
authors.affiliation: Max-Planck-Institut für Biochemie, Abt. Zellbiologie, Martinsried, FRG.
authors.forename: M
authors.initials: M
authors.lastname: Maniak
authors.forename: W
authors.initials: W
authors.lastname: Nellen
mesh_terms: D001483:Base Sequence; D015152:Blotting, Northern; D004023:Dictyostelium / Q000235:genetics*; D005246:Feedback; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D016372:RNA, Antisense / Q000235:genetics; D012331:RNA, Fungal / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D016372:RNA, Antisense; D012331:RNA, Fungal