Penicillin binding proteins of Vibrio cholerae.
Eleven penicillin binding proteins (PBPs) of Vibrio cholerae have been identified using [125I] labelled p-hydroxybenzyl penicillin (PenX). These proteins are localised in the inner membrane and have molecular weights ranging from 97,000 to 22,000. Neutral hydroxylamine released the labelled PenX from the PBPs and pretreatment with cold benzyl penicillin inhibited labelling completely. The PBP 4 is the most sensitive target for cephaloridine and aztreonam. Cephaloridine also binds to three other high molecular weight PBPs, 1, 2 and 3. Aztreonam, in addition to PBP 4, has affinity for another low molecular weight PBP, PBP 7. Mecillinam has affinity for PBPs 1, 4 and 11.
Sengupta
T K
TK
Biophysics Division, Indian Institute of Chemical Biology, Calcutta.
Chatterjee
A N
AN
Das
J
J
D001398:Aztreonam / Q000494:pharmacology; D001426:Bacterial Proteins; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002509:Cephaloridine / Q000494:pharmacology; D006602:Hexosyltransferases; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D002267:Muramoylpentapeptide Carboxypeptidase / Q000302:isolation & purification / Q000378:metabolism*; D010400:Penicillin G / Q000031:analogs & derivatives / Q000378:metabolism; D046915:Penicillin-Binding Proteins; D010406:Penicillins / Q000378:metabolism*; D010458:Peptidyl Transferases; D014734:Vibrio cholerae / Q000187:drug effects / Q000378:metabolism*
D001426:Bacterial Proteins; D002352:Carrier Proteins; D046915:Penicillin-Binding Proteins; D010406:Penicillins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; D001398:Aztreonam; D002509:Cephaloridine; D010400:Penicillin G; C037825:penicillin X