Detection of peptide-MHC class II complexes on the surface of intact cells.
The interaction of peptides with major histocompatibility complex proteins on the surface of cells is required for their recognition by T lymphocytes. Many studies characterizing the formation of peptide-MHC class II complexes have used either assays for T cell responses or for peptide binding to purified class II molecules. Recently, specific peptide-class II interactions have been demonstrated convincingly on the surface of intact cells. The effects of varying peptide and class II structure have been examined in order to identify structural requirements for binding to cell surface class II molecules and to examine the conformation adopted by immunogenic peptides when bound.
Busch
R
R
ImmuLogic Pharmaceutical Corporation, Palo Alto, CA 94304.
Rothbard
J B
JB
D000595:Amino Acid Sequence; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000954:Antigens, Surface / Q000032:analysis*; D000949:Histocompatibility Antigens Class II / Q000032:analysis* / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis* / Q000276:immunology; D012689:Sequence Homology, Nucleic Acid; D013601:T-Lymphocytes / Q000276:immunology
D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II; D010455:Peptides