Regulation of O6-methylguanine-DNA methyltransferase expression in the Burkitt's lymphoma cell line Raji.
We have investigated the expression of the DNA-repair enzyme O6-methylguanine-DNA methyltransferase in the Burkitt's lymphoma cell line Raji. An existing mutant Raji cell line which lacks thymidine kinase activity had previously been shown to be Mex- and to no longer express O6-methylguanine-DNA methyltransferase. We report here that in addition to the methyltransferase and thymidine kinase, a third enzyme with an unrelated function, galactokinase, is also not expressed in Raji cells. The control of thymidine kinase expression is post-transcriptional and it is possible that galactokinase and methyltransferase can share a common post-transcriptional regulation with thymidine kinase.
Karran
P
P
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts., Great Britain.
Stephenson
C
C
Cairns-Smith
S
S
Macpherson
P
P
D015139:Blotting, Southern; D002051:Burkitt Lymphoma; D004260:DNA Repair; D005686:Galactokinase / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008780:Methyltransferases / Q000235:genetics* / Q000378:metabolism; D019853:O(6)-Methylguanine-DNA Methyltransferase; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013937:Thymidine Kinase / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D008780:Methyltransferases; D019853:O(6)-Methylguanine-DNA Methyltransferase; D013937:Thymidine Kinase; D005686:Galactokinase