O6-alkyltransferase activity in normal human gastric mucosa.
The spectrum of activity of the DNA repair enzyme O6-alkyltransferase has been studied in a large series of normal stomachs in order to establish the baseline range of values for this enzyme. Sixty-eight patients with histologically normal stomachs were biopsied during the course of upper gastrointestinal endoscopy and the biopsies assayed for O6-alkyl-transferase activity. A wide spectrum of activity was found with values ranging from 38 fmol O6-guanine extracted/mg protein to over 400 fmol/mg. This suggests that there may be wide inter-individual differences in susceptibility to alkylating actions in the human gastric mucosa.
Dyke
G W
GW
Cancer Research Unit, University of York, Heslington, U.K.
Craven
J L
JL
Hall
R
R
Cooper
D P
DP
Soballa
G
G
Garner
R C
RC
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004247:DNA / Q000378:metabolism; D005260:Female; D005753:Gastric Mucosa / Q000201:enzymology*; D006801:Humans; D007700:Kinetics; D008297:Male; D008780:Methyltransferases / Q000378:metabolism*; D008875:Middle Aged; D019853:O(6)-Methylguanine-DNA Methyltransferase; D010865:Pilot Projects; D014316:Tritium
D014316:Tritium; D004247:DNA; D008780:Methyltransferases; D019853:O(6)-Methylguanine-DNA Methyltransferase