Bone marrow stromal elements in murine leukemia: decreased CSF-producing fibroblasts and normal IL-1 expression by macrophages.
A study of bone marrow stromal elements in murine acute myeloid leukemia (AML) was carried out. Our previous studies had indicated marrow stromal deficiency in murine AML. In the current investigation, separate stromal cells were cultured and the results obtained have shown that, while marrow stromal macrophages are normal in leukemia and express adequate amounts of IL-1, the fibroblasts are markedly reduced. However, if sufficient fibroblasts are pooled in vitro, they produce adequate amounts of CSF. Test of TNF alpha in leukemic cells CM, as possible cause of marrow stromal inhibition in leukemia, had not disclosed this cytokine. Further, it was observed that total body lethal irradiation of leukemic mice aggravates the stromal deficiency, confirming results of our previous investigations. It is concluded that bone marrow stromal deficiency in murine AML is due to decreased fibroblasts and, implicitly, reduced CSF production.
Ben-Ishay
Z
Z
Department of Anatomy & Embryology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Barak
V
V
Shoshan
S
S
Prindull
G
G
D000818:Animals; D001853:Bone Marrow / Q000473:pathology* / Q000528:radiation effects; D002452:Cell Count; D003115:Colony-Stimulating Factors / Q000096:biosynthesis*; D003470:Culture Media; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology* / Q000528:radiation effects; D006909:Hydroxyproline / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000378:metabolism / Q000473:pathology*; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism / Q000473:pathology; D008264:Macrophages / Q000378:metabolism / Q000473:pathology* / Q000528:radiation effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D014916:Whole-Body Irradiation
D003115:Colony-Stimulating Factors; D003470:Culture Media; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D006909:Hydroxyproline