Investigation of intracellular organelles in the human organ of Corti using backscattered electrons (BSE).
Using ligand-mediated osmification (OTOTO technique), human cochleae were prepared for scanning electron microscopy. The surface of the organ of Corti and the bodies of the sensory cells were observed with secondary and backscattered electrons. Detecting the backscattered electrons emitted from the depth of the cells, intracellular organelles such as subsurface cisternae, mitochondria and nuclei became visible in their reciprocal arrangement.
Reiss
G
G
Laboratory of Cell Biology and Electron Microscopy, School of Medicine, Hannover, FRG.
D006652:Histological Techniques; D006801:Humans; D006834:Hydrazines; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D009925:Organ of Corti / Q000648:ultrastructure*; D015388:Organelles / Q000648:ultrastructure*; D009993:Osmium Tetroxide
D006834:Hydrazines; C011368:thiocarbohydrazide; D009993:Osmium Tetroxide