[Sodium diphenylhydantoin changes the concentrations of DNA and proteins in the cerebrum, cerebellum and liver].
Sodium diphenylhydantoin (DFH-Na) is the drug of choice to control convulsive seizure disorders. Beneficial as well as adverse effects of DFH-Na have been reported to occur since 1938. Thus, the present article deals with the effect that 2.5, 5, 10, 15, 20 and 100 mg/kg/day (2.5, 5, 10, 15, 20 and 100) might cause on Cerebrum (C), Cerebellum (Cb) and liver (L) DNA [DNA] and Protein [Pr] concentration. Our results showed that: 1) DNA-C-14 (15, 20 and 100) were found decreased when compared to control (p less than 0.001) and [DNA]-C-30 (15, 20 and 100) as well (p less than 0.001). [Pr]-C-7, 14, 30 (2.5, 5, 10, 15, 20 and 100) showed no statistically important differences. 2) [DNA]-Cb-14 (15 and 20) were found lower than control (p less than 0.05) and [DNA]-Cb-30 (15 and 20) as well (p less than 0.05). [Pr]-Cb-14,30 (100) was found decreased (p less than 0.05). 3) [DNA]-L-14 (10, 15, 20 and 100) was found decreased when compared to control (p less than 0.001) and [DNA]-L-30 (10, 15, 20 and 100) as well (p less than 0.001). [Pr]-L-7, 14 and 30 (2.5, 5, 10, 15, 20 and 100) were found lower than control (p less than 0.05). A bimodal pattern of [DNA] of C. Cb and L was demonstrated to occur with i.p. injected DFH-Na.
Navarro-Ruíz
A
A
División de Bioquímica Farmacológica, Unidad de Investigación Biomédica de Occidente, IMSS, México.
Garzón
P
P
García-Estrada
J
J
González-Hita
M
M
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects*; D002531:Cerebellum / Q000737:chemistry; D002540:Cerebral Cortex / Q000737:chemistry; D004247:DNA / Q000032:analysis*; D005260:Female; D008099:Liver / Q000737:chemistry / Q000187:drug effects*; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis; D010672:Phenytoin / Q000494:pharmacology*; D011506:Proteins / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D009419:Nerve Tissue Proteins; D011506:Proteins; D010672:Phenytoin; D004247:DNA
El difenilhidantoinato sódico modifica la concentración de ADN y proteínas en cerebro, cerebelo e hígado.