title: Characterization of RAD4 gene required for ultraviolet-induced excision repair of Saccharomyces cerevisiae propagated in Escherichia coli without inactivation.
abstract: The previously isolated RAD4 gene designated as pPC1 from the genomic library of Saccharomyces cerevisiae (Yoon et al., 1985, Korean J. Genetics 7, 97-104) appeared to propagate in Escherichia coli and yet retained its complementing activity to rad4 mutants without inactivation. The subcloned RAD4 gene was found to be localized within a 2.5 kb DNA fragment flanking Bg1II and BamHI sites in the insert DNA, and was shown to have the same restriction map as a yeast chromosomal DNA, as determined by Southern hybridization. Tetrad analysis and pulse-field chromosome mapping have revealed that the cloned RAD4 gene can be mapped and integrated into the yeast chromosome V, the actual site of this gene. DNA-tRNA hybridization has shown that the isolated RAD4 gene did not contain a suppressor tRNA gene. These results have indicated that the pPC1 is a functional RAD4 gene playing a unique role involved in the nucleotide excision repair of yeast without any genetic change during amplification in E. coli.
authors.affiliation: Department of Zoology, College of Natural Sciences, Seoul National University, Republic of Korea.
authors.forename: I S
authors.initials: IS
authors.lastname: Choi
authors.forename: J B
authors.initials: JB
authors.lastname: Kim
authors.forename: K N
authors.initials: KN
authors.lastname: Lee
authors.forename: S D
authors.initials: SD
authors.lastname: Park
mesh_terms: D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D004260:DNA Repair / Q000528:radiation effects*; D004271:DNA, Fungal / Q000235:genetics / Q000528:radiation effects*; D004926:Escherichia coli / Q000235:genetics* / Q000528:radiation effects; D005800:Genes, Fungal; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D004271:DNA, Fungal