title: A human ubiquitin carboxyl extension protein functions in yeast.
abstract: The ability of the human ubiquitin carboxyl extension protein (HUBCEP80) to functionally replace its yeast homolog was determined in a ubi3 mutant of Saccharomyces cerevisiae. Expression of HUBCEP80 in ubi3 mutants resulted in processing of the fusion protein to produce free ubiquitin and extension protein, the latter of which localized specifically with the 40 S ribosomal subunit. Furthermore, expression of the human fusion protein completely alleviated the phenotypic deficiencies found in ubi3 mutants, including slow growth, abnormal ribosomal RNA processing, and correspondingly low levels of 40 S ribosomal subunits. Finally, expression of the extension protein alone was much less efficient in complementing the ubi3 mutant phenotype as compared with expression of the normal ubiquitin-fused extension protein. In the latter case, cells were found to contain at least 5-fold more extension protein, suggesting that ubiquitin either increased translational efficiency of the HUBCEP80 transcript or increased the stability of the processed extension protein.
authors.affiliation: ISIS Pharmaceuticals, Carlsbad, California 92008.
authors.forename: B P
authors.initials: BP
authors.lastname: Monia
authors.forename: D J
authors.initials: DJ
authors.lastname: Ecker
authors.forename: D
authors.initials: D
authors.lastname: Finley
authors.forename: S T
authors.initials: ST
authors.lastname: Crooke
mesh_terms: D002872:Chromosome Deletion; D005816:Genetic Complementation Test; D006801:Humans; D007700:Kinetics; D008970:Molecular Weight; D010957:Plasmids; D014176:Protein Biosynthesis; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development*; D014158:Transcription, Genetic
chemical_list: D011993:Recombinant Fusion Proteins