Incorporation of crystallographic temperature factors in the statistical analysis of protein tertiary structures.
A method to identify statistically significant differences between equivalent atoms in two closely related protein X-ray crystallographic structures is described. This method uses the linear relationship found between the logarithm of the distance between equivalent atoms and their mean temperature factor to determine, by linear regression, the expected difference and variance.
Bott
R
R
Genencor Inc., South San Francisco, CA.
Frane
J
J
D000818:Animals; D002417:Cattle; D002918:Chymotrypsin / Q000737:chemistry; D009113:Muramidase / Q000737:chemistry; D010741:Phospholipases A / Q000737:chemistry; D011487:Protein Conformation; D012044:Regression Analysis; D013381:Subtilisins / Q000737:chemistry; D013696:Temperature; D014357:Trypsin / Q000737:chemistry; D014961:X-Ray Diffraction
D010741:Phospholipases A; D009113:Muramidase; D013381:Subtilisins; D002918:Chymotrypsin; D014357:Trypsin