Application of reverse-DEPT polarization transfer pulse sequence to study the metabolism of carbon-13-labeled substrates in perfused organs by 1H NMR spectroscopy.
Metabolism of 13C-enriched metabolites can be advantageously studied by reverse-polarization transfer methods. In this work an improved reverse-DEPT sequence has been applied for the first time on perfused organs in a 20-mm probe. The metabolic fate of 99% enriched [2-13C]acetate perfused in excised rat liver and heart has been documented.
Desmoulin
F
F
Centre de Résonance Magnétique Biologique et Médicale, URA CNRS-1186, Faculté de Médecine de la Timone, Marseille, France.
Confort-Gouny
S
S
Masson
S
S
Bernard
M
M
Doddrell
D M
DM
Cozzone
P J
PJ
D000085:Acetates / Q000493:pharmacokinetics; D000818:Animals; D002247:Carbon Isotopes; D006321:Heart / Q000033:anatomy & histology*; D008099:Liver / Q000033:anatomy & histology*; D009682:Magnetic Resonance Spectroscopy; D051381:Rats
D000085:Acetates; D002247:Carbon Isotopes