Determination of total body water by Fourier transform infrared analysis.
A new technique for determining body water using deuterium isotope dilution and Fourier transform infrared (FTIR) analysis is described. The advantages of the FTIR over conventional dispersion and filter infrared instruments include greater flexibility through computer controlled operations and availability of 'on-line' analytical software. The technique was further improved by the development of a simple procedure for determining D2O concentration in untreated serum samples. A validation study of six normal adults showed that the fat-free-mass determined from the deuterium-space (total body water) correlated well with the results obtained by total body nitrogen (r = 0.997), total body potassium (r = 0.996) and anthropometric (r = 0.995) measurements.
Blagojevic
N
N
Australian Nuclear Science and Technology Organisation, Lucas Heights, NSW.
Allen
B J
BJ
Gaskin
K J
KJ
Baur
L A
LA
D000328:Adult; D001834:Body Water; D003903:Deuterium; D005260:Female; D005583:Fourier Analysis; D006801:Humans; D007259:Infrared Rays; D008297:Male; D008875:Middle Aged; D009584:Nitrogen / Q000032:analysis; D011188:Potassium / Q000032:analysis; D012016:Reference Values; D015203:Reproducibility of Results
D003903:Deuterium; D009584:Nitrogen; D011188:Potassium