title: [Isoenzyme spectrum and kinetic properties of pyruvate kinase from the liver of thiamine-deficient rats].
abstract: Thiamine-deficiency in animals induced by everyday subcutaneous administration of oxythiamine in a dose of 4, 40 and 100 mg/kg of weight for 10 days results in a decrease of the total activity of pyruvate kinase in the liver tissue and does not affect the mentioned index in the kidney and heart tissues. It is shown that as a result of the enzyme fractionation in the column with DEAE-cellulose the total activity of pyruvate kinase in the liver tissue of rats with thiamine-deficiency decreases due to L-isoform while the content of M-isoform remains unchanged. Thiamine deficiency does not affect kinetic characteristics of the L-isoform, extracted from the liver and this shows the absence of changes in the degree of phosphorylation of pyruvate kinase L-isoform under these conditions.
authors.forename: O V
authors.initials: OV
authors.lastname: Konovalenko
authors.forename: S S
authors.initials: SS
authors.lastname: Maglysh
authors.forename: Z V
authors.initials: ZV
authors.lastname: Gorbach
mesh_terms: D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D007527:Isoenzymes / Q000037:antagonists & inhibitors* / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D009206:Myocardium / Q000201:enzymology; D009928:Organ Specificity; D011770:Pyruvate Kinase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051381:Rats; D013832:Thiamine Deficiency / Q000201:enzymology*
chemical_list: D007527:Isoenzymes; D011770:Pyruvate Kinase
vernacular_title: Izofermentnyĭ spektr i kineticheskie svoĭstva piruvatkinazy pecheni tiamindefitsitnykh krys.