title: Subcloning and trial of expression of the protein kinase catalytic domain of RSV-src gene.
abstract: The gene of catalytic domain of the protein kinase of RSV-scr was cloned into the BamHI cloning site of translation vector pET-8c which containing T7 RNA polymerase promotor, and transformed BL21 (DE3) pLys S (Studier and Moffatt, 1986). The putative molecular weight of the protein was about 33 kd as evaluated on the basis of its nucleotide size showed the identical mobility in SDS-polyacrylamide gel electrophoresis. However, yield of protein production was not high, probably, because of its instability in Escherichia coli.
authors.affiliation: Department of Virology, Kurume University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Chinami
authors.forename: Y
authors.initials: Y
authors.lastname: Ohtsu
authors.forename: M
authors.initials: M
authors.lastname: Goto
authors.forename: K
authors.initials: K
authors.lastname: Yuge
authors.forename: H
authors.initials: H
authors.lastname: Kumashiro
authors.forename: M
authors.initials: M
authors.lastname: Shingu
mesh_terms: D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology; D015870:Gene Expression; D006801:Humans; D015688:Oncogene Protein pp60(v-src) / Q000235:genetics*; D009857:Oncogenes; D011494:Protein Kinases / Q000235:genetics*
chemical_list: D011494:Protein Kinases; D015688:Oncogene Protein pp60(v-src)