The complete nucleotide sequence of the gene coding for botulinum type C1 toxin in the C-ST phage genome.
Two DNA fragments, 3 kbp and 7.8kbp, which encode the type C1 botulinum neurotoxin gene, were obtained from toxigenic bacteriophage DNA by treatment with a restriction enzyme. They were cloned into the plasmid vectors for nucleotide sequence determination. The nucleotide sequence contained a single open reading frame coding for 1,291 amino acids corresponding to a polypeptide with a molecular weight of 149,000. The amino acid sequence of the C1 toxin has a few regions highly homologous with tetanus toxin.
Kimura
K
K
Department of Epidemiology, Hokkaido Institute of Public Health, Hokkaido University, Japan.
Fujii
N
N
Tsuzuki
K
K
Murakami
T
T
Indoh
T
T
Yokosawa
N
N
Takeshi
K
K
Syuto
B
B
Oguma
K
K
D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D001905:Botulinum Toxins / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular / Q000379:methods; D003014:Clostridium botulinum / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial; D005814:Genes, Viral; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D012689:Sequence Homology, Nucleic Acid; D015678:Viral Structural Proteins / Q000235:genetics*
D004279:DNA, Viral; D015678:Viral Structural Proteins; D001905:Botulinum Toxins