Protection from cerebral ischemic injury in gerbils with the spin trap agent N-tert-butyl-alpha-phenylnitrone (PBN).
N-Tert-butyl-alpha-phenylnitrone (PBN), a spin trap agent, reduced ischemic hippocampal damage and the associated locomotor hyperactivity in Mongolian gerbils. Cerebral ischemia was induced in unanesthetized gerbils by a bilateral 5 min occlusion of the carotid arteries. PBN (100 mg/kg, i.p.) administered 30 min prior to carotid occlusion, prevented the increase in locomotor activity observed in saline-injected ischemic animals and significantly reduced damage to the hippocampal CAI pyramidal cell layer observed 5 days post-ischemia. These findings support the hypothesis of an involvement of reactive free radicals as a significant cause of ischemia-reperfusion-induced cerebral injury and suggest that PBN may be a useful agent for the prevention of cerebral ischemic damage.
Phillis
J W
JW
Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201.
Clough-Helfman
C
C
D000818:Animals; D002545:Brain Ischemia / Q000473:pathology / Q000517:prevention & control*; D003497:Cyclic N-Oxides; D005849:Gerbillinae; D006624:Hippocampus / Q000473:pathology; D008297:Male; D009043:Motor Activity / Q000187:drug effects; D009589:Nitrogen Oxides / Q000494:pharmacology*; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D013113:Spin Labels
D003497:Cyclic N-Oxides; D009589:Nitrogen Oxides; D013113:Spin Labels; C029217:phenyl-N-tert-butylnitrone