title: Determination of the enantiomers of mefloquine in plasma and whole blood using a coupled achiral-chiral high-performance liquid chromatographic system.
abstract: A coupled achiral-chiral high-performance liquid chromatographic system has been developed for the determination of the enantiomers of mefloquine, (+)-MFQ and (-)-MFQ, in plasma and whole blood. The MFQ was separated from the interfering components in the biological matrix and quantified on a cyano-bonded phase, and the enantiomeric composition was determined on an (S)-naphthylurea chiral stationary phase. The two columns were connected by a switching valve equipped with a silica precolumn. The precolumn was used to concentrate the MFQ in the eluent from the achiral column before backflushing onto the chiral phase. The coupled-column system was validated and applied to the analysis of a pilot study of the pharmacokinetics of (+)- and (-)-MFQ in plasma and whole blood.
authors.affiliation: Pharmaceutical Division, St. Jude Children's Research Hospital, Memphis, TN 38105.
authors.forename: F
authors.initials: F
authors.lastname: Gimenez
authors.forename: R
authors.initials: R
authors.lastname: Farinotti
authors.forename: A
authors.initials: A
authors.lastname: Thuillier
authors.forename: G
authors.initials: G
authors.lastname: Hazebroucq
authors.forename: I W
authors.initials: IW
authors.lastname: Wainer
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008297:Male; D015767:Mefloquine / Q000097:blood* / Q000493:pharmacokinetics; D011446:Prospective Studies; D013237:Stereoisomerism
chemical_list: D015767:Mefloquine