title: Development of a high performance liquid chromatography method for the simultaneous measurement of prednisone and prednisolone.
abstract: An improved high performance liquid chromatographic assay (HPLC) is presented for the quantitative determination of prednisone and its main metabolite prednisolone. This HPLC assay, employing a UV-detector and dexamethasone as an internal standard, provides a selective and sensitive determination of glucocorticoids in plasma. The advantage of this method is the possibility of simultaneously measuring exogenic and endogenic glucocorticoids. The lower limit of quantification is 5 ng/ml.
authors.affiliation: Analytical Research Centre of the LAB GmbH, Neu Ulm, Fed. Rep. of Germany.
authors.forename: F
authors.initials: F
authors.lastname: Huber
authors.forename: M
authors.initials: M
authors.lastname: Wiedemann
authors.forename: G
authors.initials: G
authors.lastname: Heinrich
authors.forename: Z
authors.initials: Z
authors.lastname: Salama
authors.forename: H
authors.initials: H
authors.lastname: Jaeger
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D011239:Prednisolone / Q000097:blood* / Q000737:chemistry; D011241:Prednisone / Q000097:blood* / Q000737:chemistry; D011786:Quality Control; D013056:Spectrophotometry, Ultraviolet
chemical_list: D011239:Prednisolone; D011241:Prednisone