[The structure of carbohydrate chains of hemagglutinin and neuraminidase of influenza virus B/Leningrad/179/86].
The main surface glycoprotein, hemagglutinin (HA), was obtained by treatment of influenza virus B/Leningrad/179/86 with bromelain. Amino acid and monosaccharide compositions of HA and neuraminidase (NA, earlier isolated from the same virus) were determined, thus showing HA and NA to contain 8-10 and 2 carbohydrate chains, respectively. The carbohydrate fragments were cleaved off by the alkaline LiBH4 treatment, the oligosaccharides released were reduced with NaB3H4 and fractionated by two-step HPLC on Ultrasphere-C18 and Zorbax-NH2 columns. Some higher mannose and complex oligosaccharides were identified in both cases by comparison with nonlabelled oligosaccharides of the known structure. The data obtained show that surface glycoproteins of influenza virus A and B are rather similar with regard to structure and heterogeneity of their carbohydrate chains.
Arbatskiĭ
N P
NP
Zheltova
A O
AO
Iurtov
D V
DV
Kharitonenkov
I G
IG
Abashev
Iu P
IuP
Derevitskaia
V A
VA
Kochetkov
N K
NK
D000596:Amino Acids / Q000737:chemistry*; D001963:Bromelains; D002241:Carbohydrates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D006023:Glycoproteins / Q000737:chemistry; D006389:Hemagglutinins, Viral / Q000737:chemistry* / Q000302:isolation & purification; D009981:Influenza B virus / Q000032:analysis* / Q000201:enzymology / Q000276:immunology; D009439:Neuraminidase / Q000737:chemistry* / Q000302:isolation & purification; D009844:Oligosaccharides / Q000737:chemistry
D000596:Amino Acids; D002241:Carbohydrates; D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D009844:Oligosaccharides; D001963:Bromelains; D009439:Neuraminidase
Struktura uglevodnykh tsepeĭ gemaggliutinina i neĭraminidazy virusa grippa B/Leningrad/179/86.