Fab fragments from a monoclonal antibody against a germ tube mannoprotein block the yeast-to-mycelium transition in Candida albicans.
Fab fragments prepared from the immunoglobulin G monoclonal antibody (MAb) 4C12, which reacts with a determinant expressed on the hyphal extension of germ tubes of Candida albicans, inhibited germ tube formation, but intact MAb 4C12 did not. Indirect immunofluorescence showed a punctate binding pattern on cells incubated with Fab fragments but a confluent binding on cells incubated with intact MAb 4C12.
Casanova
M
M
Departmento de Microbiología, Facultad de Farmacia, Universitat de València, Spain.
Martínez
J P
JP
Chaffin
W L
WL
D000911:Antibodies, Monoclonal / Q000276:immunology*; D001202:Ascitic Fluid / Q000382:microbiology; D002176:Candida albicans / Q000254:growth & development* / Q000276:immunology / Q000648:ultrastructure; D002473:Cell Wall / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D008562:Membrane Glycoproteins / Q000032:analysis*; D008856:Microscopy, Fluorescence
D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D008562:Membrane Glycoproteins; C025363:mannoproteins