Induction of DNA-repair synthesis in primary rat hepatocytes by epoxides.
The genotoxicity of 10 epoxides was investigated in the UDS test with primary rat hepatocytes. The sensitivity of the assay was demonstrated using 2-acetylaminofluorence. The epoxides 1,2-epoxyoctane, 1,2-epoxydecane, epoxycyclooctane, epoxycyclododecane, (+)-limoneoxide, alpha-pinaneoxide, transstilbeneoxide, and cis-2,3-epoxysuccinic acid, which are known to be non-mutagenic in the Ames test, as well as the bacterial mutagen, 1,2-epoxyphenoxypropane did not induce UDS in primary hepatocytes of the rat. However, a positive UDS response obtained with glycidyltrimethylammonium chloride showed that metabolic inactivation of the oxirane ring in hepatocytes is influenced by further structural substituents.
von der Hude
W
W
Institut für Allgemeine Genetik, Freie Universität Berlin, Germany.
Mateblowski
R
R
Basler
A
A
D000818:Animals; D002478:Cells, Cultured; D004260:DNA Repair / Q000187:drug effects*; D004852:Epoxy Compounds / Q000494:pharmacology*; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D004852:Epoxy Compounds