title: Integration host factor affects expression of two genes at the conjugal transfer origin of plasmid R100.
abstract: Integration host factor (IHF) binds to two sites near the origin of transfer of the conjugative antibiotic resistance plasmid, R100. DNase I footprinting shows that one site is immediately adjacent to oriT and the gene X promoter, and another is adjacent to the traM promoter. A third site, known only from retardation gels, is near the traJ promoter. The relative promoter activities of genes X, traJ and traM are reduced in himA mutants (IHF-), as measured by chloramphenicol-resistance assays. Transcript analyses by Northern blots showed a reduction in size of the principal gene X and traJ transcripts in the absence of IHF.
authors.affiliation: Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas.
authors.forename: W B
authors.initials: WB
authors.lastname: Dempsey
authors.forename: B E
authors.initials: BE
authors.lastname: Fee
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002702:Chloramphenicol Resistance; D002874:Chromosome Mapping; D003227:Conjugation, Genetic; D004268:DNA-Binding Proteins / Q000235:genetics*; D003850:Deoxyribonuclease I; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D034982:Integration Host Factors; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D034982:Integration Host Factors; D003850:Deoxyribonuclease I