Temperature elevation enhances cell surface expression of measles virus fusion protein in infected cells.
Cell fusion proceeded gradually in measles virus-infected cells incubated at 35 degrees C. Shift-up of incubation temperature to 39 degrees C induced rapidly increased cell fusion in spite of the cessation of de novo synthesis of the fusion (F) protein. Pulse-chase experiments showed that there was little difference in the acquisition of immunoreactivity by haemagglutinin (H) and F proteins between the two temperatures. H protein was detected on the cell surface 60 min after the chase at either temperature. However, appearance of F protein on the cell surface took less than 3 h at 39 degrees C whereas it took 5 h at 35 degrees C. These data indicate that temperature elevation induces more efficient expression of F protein on the cell surface accompanied by marked syncytium formation in measles virus-infected cells.
Ogura
H
H
Department of Bacteriology, School of Medicine, Kanazawa University, Japan.
Sato
H
H
Kamiya
S
S
Nakamura
S
S
D002462:Cell Membrane / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D008459:Measles virus / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism; D011233:Precipitin Tests; D013696:Temperature; D013997:Time Factors; D014760:Viral Fusion Proteins / Q000378:metabolism*
D008562:Membrane Glycoproteins; D014760:Viral Fusion Proteins