Effect of cellular growth state on indices of n - 6 polyunsaturated fatty acid status in human fibroblasts.
A 2 x 2 design was employed to examine the effect of cellular growth state and medium serum concentration on potential indices of n - 6 polyunsaturated fatty acid (PUFA) status in human skin fibroblasts. The cells were cultured either as nonmultiplying cell monolayers or as medium-density, log-phase multiplying cells. An interaction of cellular growth state and medium serum concentration influenced the accumulation of 20:3(n - 9), but not 22:3(n - 9), in the cellular phospholipids. The 20:3(n - 9)/20:4(n - 6) ratio was the most sensitive index of n - 6 PUFA status; however, the ratio was significantly affected by cellular growth state. The 22:3(n - 9)/22:4(n - 6) ratio appears to be an index of n - 6 PUFA status in fibroblasts that is not significantly affected by the growth state of cells.
Bettger
W J
WJ
Department of Nutritional Sciences, College of Biological Science, University of Guelph, Ont., Canada.
Driscoll
E R
ER
Karmiol
S
S
D000818:Animals; D001790:Blood Physiological Phenomena; D002417:Cattle; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008297:Male
D003470:Culture Media; D005231:Fatty Acids, Unsaturated