Evaluation of the stick enzyme immunoassay in Caranx sp. and Seriola dumerili associated with ciguatera.
The stick enzyme immunoassay (S-EIA) using monoclonal antibody to ciguatoxin (MAb-CTX) was used to examine clinically implicated fish and to pre-screen two species of fish, Caranx sp. (ulua or jack) and Seriola dumerili (kahala or amberjack), supplied by sports fishermen. All of the clinically implicated fish from the Department of Health gave S-EIA values greater than or equal to 1.3. The Caranx sp. and Seriola dumerili considered safe (less than or equal to 1.2 value) and consumed after testing gave no false-negative results. The S-EIA procedure using MAb-CTX proved to be specific, sensitive, and simple to use in the laboratory. It also proved to be useful in screening two large carnivorous fish for ciguatoxin and related polyethers prior to consumption.
Hokama
Y
Y
Department of Pathology, John A. Burns School of Medicine, University of Hawaii, Manoa, Honolulu 96822.
Asahina
A Y
AY
Hong
T W
TW
Shang
E S
ES
Miyahara
J T
JT
D000818:Animals; D000911:Antibodies, Monoclonal; D002922:Ciguatoxins / Q000009:adverse effects / Q000276:immunology*; D005399:Fishes / Q000276:immunology*; D006254:Hawaii; D006801:Humans; D007124:Immunoenzyme Techniques; D013997:Time Factors
D000911:Antibodies, Monoclonal; D002922:Ciguatoxins