New procedure for direct bioautographic TLC assay as applied to a tincture of Ranunculus bulbosus.
A new procedure for TLC bioautographic assay using a flooding method to inoculate the developed plates is described. Details about several microbiological parameters allow good reproducibility for this method which appears rapid, easy to perform and requiring no specialized equipment. Significantly results obtained with a commercial tincture of Ranunculus bulbosus illustrate this method which can be extended to complex mixtures of natural products in order to isolate active fractions.
Didry
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Laboratoires de Pharmacognosie, Faculté de Pharmacie de Lille, France.
Dubreuil
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L
Pinkas
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M
D001681:Biological Assay; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002855:Chromatography, Thin Layer; D005658:Fungi / Q000187:drug effects; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000032:analysis* / Q000494:pharmacology; D010946:Plants, Medicinal / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D010936:Plant Extracts