title: Liquid chromatography/mass spectrometry of plasma glucose and secreted glucuronate for metabolic studies in humans.
abstract: Negative ion thermospray liquid chromatographic/mass spectrometric methods have been developed for the determination of isotopic enrichments for glucose and acetaminophen-uridine diphosphate glucuronate from (1-2H1)glucose, (1-2H1)galactose and (2-13C)acetate in humans. The error of estimate ranged from below 1% to 5%. The advantages of the method include fast analysis (up to 35 per hour), eased sample preparation, good precision, sensitivity comparable with gas chromatography/mass spectrometry and better than with isotope ratio mass spectrometry.
authors.affiliation: Clinical Mass Spectrometry Facility, Children's Hospital Oakland Research Institute, California 94609.
authors.forename: S
authors.initials: S
authors.lastname: Reid
authors.forename: C
authors.initials: C
authors.lastname: Shackleton
authors.forename: K
authors.initials: K
authors.lastname: Wu
authors.forename: S
authors.initials: S
authors.lastname: Kaempfer
authors.forename: M K
authors.initials: MK
authors.lastname: Hellerstein
mesh_terms: D001786:Blood Glucose / Q000032:analysis*; D002853:Chromatography, Liquid; D005965:Glucuronates / Q000097:blood*; D006801:Humans; D013058:Mass Spectrometry
chemical_list: D001786:Blood Glucose; D005965:Glucuronates