title: Formation of the lignan, (-) secoisolariciresinol, by cell free extracts of Forsythia intermedia.
abstract: A crude cell-free extract from Forsythia intermedia catalyses the formation of (-)secoisolariciresinol, and not its (+) enantiomer, when incubated with coniferyl alcohol in the presence of NAD(P)H and H2O2. This is the first report of an enzyme reaction specifically involved in the coupling reaction between two phenylpropanoid monomers to afford an optically pure lignan.
authors.affiliation: Commonwealth Center for Wood Science and Technology/Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg 24061.
authors.forename: T
authors.initials: T
authors.lastname: Umezawa
authors.forename: L B
authors.initials: LB
authors.lastname: Davin
authors.forename: N G
authors.initials: NG
authors.lastname: Lewis
mesh_terms: D002072:Butylene Glycols / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002474:Cell-Free System; D017705:Lignans; D008031:Lignin / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D013058:Mass Spectrometry; D015394:Molecular Structure; D010944:Plants / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
chemical_list: D002072:Butylene Glycols; D017705:Lignans; D008031:Lignin; C060283:secoisolariciresinol