Metabolism of ketone bodies by skeletal muscle in starvation and uncontrolled diabetes.
Previous studies have suggested that skeletal muscle may be responsible for as much as 25% of ketone body (KB) production in poorly controlled diabetes. In the present studies, acetoacetate (AcAc) and beta-hydroxybutyrate production was quantitated in the canine hindlimb from surgically placed arterial and venous catheters in conscious insulin-withdrawn diabetic (n = 5) and 4-day fasted (n = 7) dogs. A two-pool modeling technique, using simultaneous infusions of 13C acetoacetate and 14C beta-hydroxybutyrate (beta OHB) was employed to quantitate total body and hindlimb KB kinetics. Total KB production was 9.4 and 39.3 mumol.kg-1.min-1 in the fasted and diabetic animals, respectively. Hindlimb KB production was negligible in both groups. The two-pool model estimates of hindlimb KB utilization were similar to the values obtained by an arterial-venous difference calculation. In conclusion, the hindlimb does not contribute to de novo synthesis of KBs in either fasted or diabetic dogs. Since species differences in KB metabolism occur, it is possible that muscle may be a site for KB production in humans.
Bailey
J W
JW
Department of Medicine, Mayo Clinic, Rochester, MN 55905.
Haymond
M W
MW
Miles
J M
JM
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004285:Dogs; D005215:Fasting; D006614:Hindlimb; D007657:Ketone Bodies / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*
D007657:Ketone Bodies