Analysis of junctional diversity during B lymphocyte development.
Immunoglobulin rearrangement is central to generating antibody diversity because of heterogeneity generated during recombination by deletion or addition of nucleotides at coding joints by the recombinase machinery. Examination of these junctional modifications revealed that the addition of nongermline-encoded nucleotides was more prevalent in adult versus fetal B cells, thus partially limiting the fetal antibody repertoire. In contrast, deletion of nucleotides occurs equivalently in B cells at different stages of development and at different points in B cell ontogeny. Finally, the bias in murine immunoglobulins for one DH segment reading frame occurs at the DHJH intermediate.
Meek
K
K
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235.
D000375:Aging / Q000235:genetics / Q000276:immunology; D000818:Animals; D000831:Animals, Newborn; D000916:Antibody Diversity; D001402:B-Lymphocytes / Q000276:immunology*; D015139:Blotting, Southern; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D015322:Gene Rearrangement, B-Lymphocyte; D007133:Immunoglobulin Joining Region / Q000235:genetics*; D051379:Mice; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D015183:Restriction Mapping
D007133:Immunoglobulin Joining Region