[Cloning and expression of the gene for diphtheria toxin and its subunits in Escherichia coli].
The results of cloning Corynebacterium diphtheriae phi 984 tox gene and its A and B subunits in Escherichia coli are presented. Regulatory sequences of tox gene are capable to promote effective expression in E. coli cells. A set of recombinant plasmids has been obtained which can determine the synthesis of A and B individual subunits and are suitable for constructing immunotoxins by gene engineering. The diphtheria toxin of 62 kDa synthesized in E. coli has enzymatic activity and reacts with antitoxin sera. Some sites for E. coli proteases are present in tox-specific polypeptides.
Garaev
M M
MM
Bobkova
M R
MR
Bobkov
A F
AF
Lukashevich
N V
NV
Kazennova
E V
EV
D001426:Bacterial Proteins / Q000096:biosynthesis; D003001:Cloning, Molecular; D004167:Diphtheria Toxin / Q000737:chemistry / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D046911:Macromolecular Substances; D008970:Molecular Weight; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis; D011995:Recombination, Genetic; D015183:Restriction Mapping
D001426:Bacterial Proteins; D004167:Diphtheria Toxin; D046911:Macromolecular Substances; D011994:Recombinant Proteins
Klonirovanie i ékspressiia gena difteriĭnogo toksina i ego otdel'nykh sub''edinits v bakteriiakh Escherichia coli.