Isolation and sequencing of mouse angiogenin DNA.
The mouse genomic DNA for angiogenin, a potent blood vessel inducing protein, has been isolated from a bacteriophage library using the human angiogenin gene as a probe. The 1129 bp fragment contains 499 bp in the 5' flanking region, 192 bp in the 3' flanking region, and 438 bp coding for the mature protein (121 amino acids) and signal peptide (24 amino acids). Potential TATA box and AATAAA polyadenylation sequences are present, and a consensus sequence for an intron 3' boundary occurs 16 bp upstream of the Met-(24) codon, suggesting the presence of an intron in the 5' region. The protein sequence inferred from the DNA is 76% identical to that of human angiogenin, and matches the sequences obtained previously from tryptic peptides of a serum-derived mouse angiogenin. The critical catalytic residues of human angiogenin are conserved in the mouse protein, as are the six cysteines necessary for disulfide bond formation.
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Center for Biochemical and Biophysical Sciences and Medicine Harvard Medical School, Boston, Massachusetts 02115.
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D000595:Amino Acid Sequence; D043925:Angiogenesis Inducing Agents / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D015342:DNA Probes; D015723:Gene Library; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011506:Proteins / Q000235:genetics*; D015183:Restriction Mapping; D012259:Ribonuclease, Pancreatic; D012689:Sequence Homology, Nucleic Acid
D043925:Angiogenesis Inducing Agents; D015342:DNA Probes; D011506:Proteins; D004247:DNA; C046366:angiogenin; D012259:Ribonuclease, Pancreatic