title: Direct sectioning of unembedded cartilage: a simple method for microscopical and histochemical studies on chondrocytes and extracellular matrix.
abstract: On account of the rigidity and compact structure of the hyaline cartilage, unfixed or formaldehyde fixed samples of this tissue can be directly sectioned by using a conventional ultramicrotome and a glass knife. This simple method allows to obtain microscopical sections from unembedded cartilage blocks, which show a well preserved histological structure and are very suitable to carry out morphological and histochemical studies on chondrocytes and cartilaginous matrix.
authors.affiliation: Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
authors.forename: J C
authors.initials: JC
authors.lastname: Stockert
authors.forename: P
authors.initials: P
authors.lastname: Del Castillo
mesh_terms: D000818:Animals; D002356:Cartilage / Q000033:anatomy & histology* / Q000737:chemistry / Q000166:cytology; D005109:Extracellular Matrix / Q000737:chemistry / Q000648:ultrastructure; D006651:Histocytochemistry; D008867:Microtomy / Q000379:methods*