The urea-soluble low molecular weight cuticle proteins from the different developmental stages of Dacus oleae.
The cuticle proteins of the insect Dacus oleae have been isolated by extraction with a solution of 7 M urea. The affinity properties of cuticle proteins, isolated from the third instar larvae (L3DCPs 1-7), to chitin have been studied. Purified cuticle antigens were polymerized by glutaraldehyde and used for raising antibodies. The developmental appearance of the cuticle proteins has been studied by two-dimensional electrophoresis.
Souliotis
V L
VL
National Hellenic Research Foundation, University of Athens, Greece.
Patrinou-Georgoula
M
M
Zongza
V
V
Dimitriadis
G J
GJ
D000818:Animals; D000906:Antibodies; D002686:Chitin; D002846:Chromatography, Affinity; D004175:Diptera / Q000032:analysis / Q000254:growth & development*; D015180:Electrophoresis, Gel, Two-Dimensional; D019476:Insect Proteins; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification*; D012995:Solubility; D014508:Urea
D000906:Antibodies; D019476:Insect Proteins; D011506:Proteins; C047663:cuticle proteins, insects; D002686:Chitin; D014508:Urea