title: Incubation of platelet concentrates before transfusion does not improve posttransfusion recovery.
abstract: Incubation of stored platelet concentrates (PCs) at 37 degrees C for 1 hour has been reported to result in a better morphology score and improved platelet recovery. A study was conducted in adult patients with leukemia to determine whether incubation of stored PCs results in an improved platelet recovery as measured by 10-minute posttransfusion corrected count increments (CCI). Eligible patients had platelet counts of less than 30,000 per microL and were clinically stable. Patients were transfused with 6 to 10 units of PC stored for 3 days (15 studies) or 4 days (5 studies). Platelets were pooled and then split in two equal volumes so that each patient received two sequential half-transfusions, one incubated at 37 degrees C for 1 hour and the other kept at 22 degrees C for 1 hour. Patients were randomized as to which half-transfusion was received first. The mean CCI of the incubated half-transfusions was 13.6 x 10(3) when they were given first and 14.5 x 10(3) when given second; this was not significantly different from the mean CCI of the nonincubated half-transfusions: 13.8 x 10(3) when they were given first and 13.8 x 10(3) when given second. In contrast to earlier reports, it can be concluded that incubation of pooled PCs does not improve platelet recovery.
authors.affiliation: Division of Hematologic Malignancies, University of Maryland Cancer Center, Baltimore.
authors.forename: M A
authors.initials: MA
authors.lastname: Hussein
authors.forename: C A
authors.initials: CA
authors.lastname: Schiffer
authors.forename: E J
authors.initials: EJ
authors.lastname: Lee
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000502:physiology; D001793:Blood Preservation; D001803:Blood Transfusion; D006801:Humans; D008533:Megakaryocytes / Q000473:pathology; D010976:Platelet Count; D017713:Platelet Transfusion; D013921:Thrombocytopenia / Q000097:blood / Q000628:therapy