Subcloning and trial of expression of the protein kinase catalytic domain of RSV-src gene.
The gene of catalytic domain of the protein kinase of RSV-scr was cloned into the BamHI cloning site of translation vector pET-8c which containing T7 RNA polymerase promotor, and transformed BL21 (DE3) pLys S (Studier and Moffatt, 1986). The putative molecular weight of the protein was about 33 kd as evaluated on the basis of its nucleotide size showed the identical mobility in SDS-polyacrylamide gel electrophoresis. However, yield of protein production was not high, probably, because of its instability in Escherichia coli.
Chinami
M
M
Department of Virology, Kurume University School of Medicine, Japan.
Ohtsu
Y
Y
Goto
M
M
Yuge
K
K
Kumashiro
H
H
Shingu
M
M
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology; D015870:Gene Expression; D006801:Humans; D015688:Oncogene Protein pp60(v-src) / Q000235:genetics*; D009857:Oncogenes; D011494:Protein Kinases / Q000235:genetics*
D011494:Protein Kinases; D015688:Oncogene Protein pp60(v-src)