title: Maturation-specific type II cyclic AMP-dependent protein kinase in goat sperm plasma membrane.
abstract: Highly purified plasma membranes (PMs) isolated by aqueous two-phase polymer methods from goat sperm undergoing epididymal maturation, have been analyzed for the isoenzymes of cyclic AMP-dependent protein kinase (RC). The mature and the immature spermatozoa showed profound differences in the profile of the isoenzymes of RC solubilized from the isolated PMs with 0.1% Triton X-100. The immature sperm PM consists of only type I RC in contrast to the mature sperm membrane which possesses both the type I and II isoenzymes. The type II kinase represents nearly 30% of the total membrane-bound RC of the mature cells. The analysis of the surface topography of these isoenzymes of the maturing spermatozoa by using diazonium salt of sulfanilic acid as the surface probe shows that the PM-bound RC(s) are oriented primarily on the external surface of these intact cells. The data demonstrate that type II RC is a maturation-specific ecto-kinase as it appears on the sperm surface specifically during the maturation of spermatozoa in the epididymis.
authors.affiliation: Indian Institute of Chemical Biology, Calcutta.
authors.forename: C S
authors.initials: CS
authors.lastname: Dey
authors.forename: G C
authors.initials: GC
authors.lastname: Majumder
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D006041:Goats; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D011494:Protein Kinases / Q000378:metabolism*; D013079:Sperm Maturation / Q000502:physiology; D013094:Spermatozoa / Q000201:enzymology*
chemical_list: D007527:Isoenzymes; D011494:Protein Kinases