The difference in murine CDC2 kinase activity between cytoplasmic and nuclear fractions during the cell cycle.
The mouse analog of yeast CDC2+ kinase was detected in the cytoplasmic and nuclear fractions of cultured mouse FM3A cells. Its activity in the nuclear fraction increased in the G2/M phase became seven times higher than that in the G1/S phase, while the activity in the cytoplasmic fraction remained was almost constant from the G1/S to G1 phases. The activity in the cytoplasmic fraction was similar to that in the nuclear fraction in the G2/M phase. The amount of the enzyme remained almost constant during the cell cycle in both the nuclear and cytoplasmic fractions. These findings suggest that the cytoplasmic enzyme might play an independent role in the cell cycle.
Yasuda
H
H
Division of Biology, Faculty of Pharmaceutical Sciences, Kanazawa University, Ishikawa, Japan.
Kamijo
M
M
Honda
R
R
Nagahara
M
M
Ohba
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D016203:CDC2 Protein Kinase / Q000378:metabolism*; D002453:Cell Cycle; D002460:Cell Line; D002467:Cell Nucleus / Q000201:enzymology*; D003593:Cytoplasm / Q000201:enzymology; D003703:Demecolcine / Q000494:pharmacology; D008325:Mammary Neoplasms, Experimental; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis; D011494:Protein Kinases / Q000378:metabolism*; D013379:Substrate Specificity
D010455:Peptides; D011494:Protein Kinases; D016203:CDC2 Protein Kinase; D003703:Demecolcine