Vaccinia virus gene D7R encodes a 20,000-dalton subunit of the viral DNA-dependent RNA polymerase.
The polypeptide encoded by the vaccinia virus open reading frame D7R was synthesized in bacteria. Immunization of rabbits with the polypeptide resulted in antibodies that specifically recognized a virion polypeptide of 20,000 daltons. The immunoreactivity with the 20,000-dalton polypeptide was found to coincide with the virion-associated DNA-dependent RNA polymerase through DEAE-cellulose chromatography and glycerol gradient sedimentation. These results argue that the product of the vaccinia open reading frame D7R is a subunit of the viral RNA polymerase.
Quick
S D
SD
Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907.
Broyles
S S
SS
D012321:DNA-Directed RNA Polymerases / Q000096:biosynthesis / Q000235:genetics*; D005814:Genes, Viral; D008970:Molecular Weight; D016366:Open Reading Frames; D014616:Vaccinia virus / Q000201:enzymology / Q000235:genetics*; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics*; D014771:Virion / Q000201:enzymology / Q000235:genetics
D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases