title: Protein-energy requirements of prepubertal school-age boys determined by using the nitrogen-balance response to a mixed-protein diet.
abstract: Short-term nitrogen-balance response to a vegetable-protein mixed diet and to a milk-egg protein diet was tested in eight healthy male children 8-10 y old. They received 96, 128, 160, and 192 mg N.kg body wt-1.d-1 for 10 d while on the mixed diet and 160 mg N.kg-1.d-1 on the milk-egg diet. The mean regression equation was nitrogen balance = 0.52 (nitrogen intake) -68.3, all in mg N.kg-1.d-1. Apparent nitrogen digestibility was 82% and 86% for the mixed and milk-egg diets, respectively, when the subjects received 160 mg N.kg-1.d-1. Mean nitrogen intake for satisfactory nitrogen retention on the mixed diet was 150 mg N.kg-1.d-1; the suggested protein recommended allowance to cover 97.5% of the population was derived by using a CV of 12.5% is 1.2 g protein.kg-1.d-1. We conclude that present recommendations are adequate for short-term nitrogen retention; long-term studies are needed to evaluate the chronic safety of this protein allowance.
authors.affiliation: Instituto de Nutricion y Tecnologia de la Alimentos (INTA), University of Chile, Santiago.
authors.forename: V
authors.initials: V
authors.lastname: Gattas
authors.forename: G A
authors.initials: GA
authors.lastname: Barrera
authors.forename: J S
authors.initials: JS
authors.lastname: Riumallo
authors.forename: R
authors.initials: R
authors.lastname: Uauy
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000886:Anthropometry; D001827:Body Height; D001835:Body Weight; D002648:Child; D004044:Dietary Proteins / Q000008:administration & dosage / Q000378:metabolism*; D004063:Digestion; D004531:Eggs; D002149:Energy Intake; D006801:Humans; D008297:Male; D008892:Milk; D009584:Nitrogen / Q000378:metabolism*; D009751:Nutritional Requirements; D011897:Random Allocation; D012044:Regression Analysis; D014675:Vegetables
chemical_list: D004044:Dietary Proteins; D009584:Nitrogen