Verapamil-induced creatine kinase loss from rat slow and fast muscles.
The purpose of this study was to determine the dose-response relationship between verapamil HCl and creatine kinase (CK) release from isolated rat slow (soleus, S) and fast (flexor digitorum profundus, F) skeletal muscles. Total CK activity in F muscle was approximately 2x greater than in S muscle. For both S and F muscles, CK release as a function of verapamil concentration in the incubation medium was linear (r = 0.94); the slope of the line for F muscle was 4.5x that of the S muscle. S and F muscles lost up to 38-44% of their total CK activity during the 2 hr incubation. The CK activity lost to the medium was 100% of the MM isoform. The mechanism of the enzyme release is not known.
Glenn
G M
GM
Miami Children's Hospital, FL 33155.
Hayes
D A
DA
Armstrong
R B
RB
D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000378:metabolism; D009132:Muscles / Q000187:drug effects / Q000201:enzymology*; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D014700:Verapamil / Q000494:pharmacology*
D007527:Isoenzymes; D014700:Verapamil; D003402:Creatine Kinase