title: Rapid transformation of [3H]cholesteryl ester in rat high-density lipoprotein: in vivo and in vitro studies.
abstract: [24,25-3H]Cholesteryl ester-labeled rat high-density and low-density lipoproteins were administered to recipient rats. Following death of the rats, a major portion of the radioactivity in administered [3H]cholesteryl ester-high-density lipoprotein rapidly appeared in less dense [3H]cholesteryl ester-lipoproteins and was isolated with the low-density lipoprotein fraction. The specific activity of the esterified cholesterol in the product lipoproteins found with the low-density lipoproteins exceeded that of the precursor high-density lipoproteins. In vitro, the addition of [3H]cholesteryl ester-high-density lipoprotein to plasma resulted in a five- to six-fold increase in radioactivity recovered in the low-density lipoprotein. These results demonstrate that, under a variety of experimental conditions, isolated high-density lipoprotein particles (both in vitro and in vivo) tend to become larger and less dense. Rapid changes in the density of lipoproteins labeled with [3H]cholesteryl ester must be considered when interpreting physiologic studies using this label.
authors.affiliation: Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York.
authors.forename: I J
authors.initials: IJ
authors.lastname: Goldberg
authors.forename: R S
authors.initials: RS
authors.lastname: Rosenfeld
authors.forename: I
authors.initials: I
authors.lastname: Paul
authors.forename: L K
authors.initials: LK
authors.lastname: Miller
authors.forename: M L
authors.initials: ML
authors.lastname: Tiell
mesh_terms: D000818:Animals; D002788:Cholesterol Esters / Q000097:blood / Q000378:metabolism*; D007700:Kinetics; D008075:Lipoproteins, HDL / Q000097:blood / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000097:blood / Q000378:metabolism; D051381:Rats
chemical_list: D002788:Cholesterol Esters; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL