A common motif of two adjacent phosphoserines in bovine, rabbit and human cardiac troponin I.
From rabbit and human cardiac troponin I N-terminal mono and bisphosphorylated peptides were isolated which were obtained from Lys-C proteinase digests. Two adjacent phosphoserine residues could be localized in each phosphopeptide following further tryptic digestion. The previously published sequence of rabbit cardiac troponin I had to be corrected. Two adjacent phosphoserine residues are a common motif in the very similar sequences of bovine, rabbit and human cardiac troponin I. The N-terminal sequences are: AcADRSGGSTAG DTVPAPPPVR RRS(P)S(P)ANYRAY ATEPHAK (bovine), AcADESTDA-AG EARPAPAPVR RRS(P)S(P)ANYRAY ATEPHAK (rabbit), (Ac,A,D/N,G,S,S,D/N,A,A,R) EPRPAPAPIR RRS(P)S(P)-NYRAY ATEPHAK (human).
Mittmann
K
K
Institut für Physiologische Chemie, Abteilung für Biochemie Supramolekularer Systeme, Ruhr-Universität Bochum, FRG.
Jaquet
K
K
Heilmeyer
L M
LM
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D006801:Humans; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D010446:Peptide Fragments / Q000302:isolation & purification; D010768:Phosphoserine / Q000032:analysis*; D011817:Rabbits; D012689:Sequence Homology, Nucleic Acid; D014336:Troponin / Q000737:chemistry*; D019210:Troponin I; D014357:Trypsin
D000596:Amino Acids; D010446:Peptide Fragments; D014336:Troponin; D019210:Troponin I; D010768:Phosphoserine; D014357:Trypsin