title: Adherence of human neutrophils changes Ca2+ signaling during activation with opsonized particles.
abstract: Changes in the cytosolic free Ca2+ concentration ([Ca2+]i) upon activation of human neutrophils by opsonized particles (serum-treated zymosan; STZ) were evaluated by three different methods: (i) measurement of total fluorescence changes in indo-1 loaded neutrophils activated in suspension; (ii) measurement of fluorescence changes in individual indo-1 loaded neutrophils in a flow cytometer and (iii) measurement of fluorescence changes in individual fura-2 loaded neutrophils adherent to serum-coated coverslips. Our study shows that the opsonized particle-induced change in [Ca2+]i in neutrophils is altered during adherence of the cells to a serum-coated surface. These observations might be of importance for neutrophil function in vivo, since adherence is a prerequisite for diapedesis and chemotaxis.
authors.affiliation: Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, University of Amsterdam, The Netherlands.
authors.forename: L
authors.initials: L
authors.lastname: Koenderman
authors.forename: A T
authors.initials: AT
authors.lastname: Tool
authors.forename: B
authors.initials: B
authors.lastname: Hooybrink
authors.forename: D
authors.initials: D
authors.lastname: Roos
authors.forename: C A
authors.initials: CA
authors.lastname: Hansen
authors.forename: J R
authors.initials: JR
authors.lastname: Williamson
authors.forename: A J
authors.initials: AJ
authors.lastname: Verhoeven
mesh_terms: D002118:Calcium / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D009504:Neutrophils / Q000502:physiology*; D009895:Opsonin Proteins; D010587:Phagocytosis / Q000502:physiology*; D015398:Signal Transduction
chemical_list: D009895:Opsonin Proteins; D002118:Calcium