[Identification and susceptibility of clinically isolated yeast-like fungi].
We studied the identification and susceptibility of clinically isolated yeast-like fungi at Showa University Hospital from April 1988 to March 1989. Clinically significant of yeast-like fungi were observed in 7.1% of specimens from outpatients, 13.0% of inpatients. In both outpatients and inpatients, yeast-like fungi were isolated mainly from sputum and urine. But, one third of them were considered as non-pathogenic and not identified. The species of isolates were, Candida albicans 57%, Candida tropicalis 14% and Candida glabrata 8% in both inpatients and outpatients, and these species shared most part. The isolation frequency of Candida parapsilosis was higher in blood and cerebrospinal fluid (CSF) specimen than the others. The susceptibility test by agar dilution method indicated most of the isolates were susceptible to Amphotericin B and Miconazole (MIC less than or equal to 25 micrograms/ml). There was no difference in MIC between predominantly isolated fungi and commonly isolated fungi. Notably, isolates from blood and CSF showed a significant high tolerance against Amphotericin B and Miconazole than from the other specimens. The MICs of Fluconazole were shown to be very high (greater than 100 micrograms/ml) in normal Sabouraud agar, were decreased dose-dependently by human sera in the medium. These findings indicated the component(s) of sera enhanced the anti-fungal activity of Fluconazole.
Fukuchi
K
K
Department of Clinical Pathology, Showa University, Tokyo.
Tanaka
Y
Y
Oguro
T
T
Wakuta
R
R
Sugita
M
M
Takagi
Y
Y
Gomi
K
K
D000666:Amphotericin B / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects / Q000302:isolation & purification*; D015725:Fluconazole / Q000494:pharmacology; D006801:Humans; D008825:Miconazole / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D015003:Yeasts / Q000187:drug effects / Q000302:isolation & purification*
D000935:Antifungal Agents; D008825:Miconazole; D000666:Amphotericin B; D015725:Fluconazole