Simultaneous determination of neutral and amino sugars in biological materials.
A method is described for the simultaneous analysis of nine neutral and three amino sugars. Mixtures of standard sugars and biological samples were acid hydrolyzed with a two step Saeman procedure, neutralized with BaCO3, reduced with sodium borohydride, acidified, evaporated and alditol acetates prepared. Baseline resolution was achieved on a glass-capillary SP-2340 column in ca. 52 min. Reproducibility, response factors and hydrolysis losses were determined. Quantitation was linear over the range of 10-20 micrograms/ml to 2000 micrograms/ml. Conditions were defined for the reproducible quantitation of muramic acid.
Kraus
R J
RJ
Department of Nutritional Sciences, University of Wisconsin-Madison 53706.
Shinnick
F L
FL
Marlett
J A
JA
D000606:Amino Sugars / Q000032:analysis*; D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods*; D004064:Digestive System / Q000737:chemistry; D005243:Feces / Q000737:chemistry; D006801:Humans; D008297:Male; D009112:Muramic Acids / Q000032:analysis; D051381:Rats
D000606:Amino Sugars; D002241:Carbohydrates; D009112:Muramic Acids