title: Cryptic Met-enkephalin in adrenal and portal vein during splanchnic artery occlusion shock in cats.
abstract: Adrenal vein (AD), portal vein (PV), and femoral artery (FA) plasma levels of immunoreactive (IR) Met-enkephalin pentapeptide (ME) and extended ME-IR forms, obtained after sequential incubation of plasma with trypsin and carboxypeptidase B, were examined in 4 cats during splanchnic artery occlusion shock at baseline (S1), during early shock (S2), late shock (S3), and after naloxone (1 mg/kg, i.v.) administration (S4). Early shock (S2) led to a significant increase in levels of extended and fully processed Met-enkephalin IR at all 3 collection sites (AD, PV, FA) without a change in proportional levels of extended Met-enkephalin IR to the pentapeptide IR (ME). Naloxone administration during late shock (S4), however, resulted in a disproportionate increase (150-fold from baseline) in adrenal vein plasma levels of extended Met-enkephalin IR forms, as compared to ME IR (23-fold). In contrast, no changes in plasma levels occurred in PV and FA.
authors.affiliation: Department of Anesthesiology, Mayo Clinic, Rochester, MN 55905.
authors.forename: D M
authors.initials: DM
authors.lastname: Gaumann
authors.forename: T L
authors.initials: TL
authors.lastname: Yaksh
authors.forename: D L
authors.initials: DL
authors.lastname: Lucas
mesh_terms: D000311:Adrenal Glands / Q000098:blood supply*; D000818:Animals; D001158:Arteries; D002415:Cats; D004744:Enkephalin, Methionine / Q000097:blood*; D008026:Ligation; D009270:Naloxone / Q000494:pharmacology; D011169:Portal Vein; D011863:Radioimmunoassay; D012769:Shock / Q000097:blood* / Q000209:etiology; D013152:Splanchnic Circulation; D013997:Time Factors; D014680:Veins
chemical_list: D009270:Naloxone; D004744:Enkephalin, Methionine