title: [Cellular transformation in an MDCK culture caused by the agent of amyotrophic leukospongiosis].
abstract: The possibility of infecting epithelioid MDCK cell culture with the agent of amyotrophic leukospongiosis (AL) was shown. Various morphofunctional changes were observed in the infected cells. In the first subpassage, the saturation density and yields of cells increased, while their mitotic activity decreased and adhesive properties changed. In the second subpassage, the cell morphology and monolayer architectonics changed, the cells losing the ability to form a confluent monolayer. The infected cultures died in further subpassages. Clinical signs of the disease and morphological changes in the CNS typical of AL were observed in the animals infected with a homogenate of cells and culture fluid from the AL-agent-infected cultures.
authors.forename: V I
authors.initials: VI
authors.lastname: Votiakov
authors.forename: I R
authors.initials: IR
authors.lastname: Erokhina
authors.forename: Iu M
authors.initials: IuM
authors.lastname: Arkatov
mesh_terms: D000818:Animals; D001921:Brain / Q000382:microbiology; D001927:Brain Diseases / Q000382:microbiology* / Q000473:pathology; D002460:Cell Line; D002472:Cell Transformation, Viral; D002478:Cells, Cultured / Q000382:microbiology / Q000473:pathology; D004195:Disease Models, Animal; D004285:Dogs; D006168:Guinea Pigs; D012692:Serial Passage; D012897:Slow Virus Diseases / Q000382:microbiology* / Q000473:pathology
vernacular_title: Obuslovlennaia agentom amiotroficheskogo leĭkospongioza transformatsiia kletok v kul'ture MDCK.