title: x-y-recording in transmission electron microscopy. A versatile and inexpensive interface to personal computers with application to stereology.
abstract: An interface for IBM XT/AT-compatible computers is described which has been designed to read the actual specimen stage position of electron microscopes. The complete system consists of (i) optical incremental encoders attached to the x- and y-stage drivers of the microscope, (ii) two keypads for operator input, (iii) an interface card fitted to the bus of the personal computer, (iv) a standard configuration IBM XT (or compatible) personal computer optionally equipped with a (v) HP Graphic Language controllable colour plotter. The small size of the encoders and their connection to the stage drivers by simple ribbed belts allows an easy adaptation of the system to most electron microscopes. Operation of the interface card itself is supported by any high-level language available for personal computers. By the modular concept of these languages, the system can be customized to various applications, and no computer expertise is needed for actual operation. The present configuration offers an inexpensive attachment, which covers a wide range of applications from a simple notebook to high-resolution (200-nm) mapping of tissue. Since section coordinates can be processed in real-time, stereological estimations can be derived directly "on microscope". This is exemplified by an application in which particle numbers were determined by the disector method.
authors.affiliation: Zentrum Anatomie, Universität Göttingen, F.R.G.
authors.forename: M
authors.initials: M
authors.lastname: Rickmann
authors.forename: L
authors.initials: L
authors.lastname: Siklós
authors.forename: F
authors.initials: F
authors.lastname: Joó
authors.forename: J R
authors.initials: JR
authors.lastname: Wolff
mesh_terms: D000818:Animals; D005728:Ganglia, Sympathetic / Q000648:ultrastructure*; D007091:Image Processing, Computer-Assisted; D008838:Microcomputers; D008854:Microscopy, Electron; D051381:Rats; D013569:Synapses / Q000648:ultrastructure*