title: Determination of metrifonate and dichlorvos in whole blood using gas chromatography and gas chromatography-mass spectrometry.
abstract: Analytical methods for determining metrifonate and dichlorvos in whole blood and a sampling procedure suitable for pharmacokinetic studies in man are described. Metrifonate concentrations were determined after chloroform extraction using gas chromatography-nitrogen-phosphorus detection. The within-assay coefficients of variation were 4 and 9% at 19.4 and 0.8 mumol/l (limits of determination), respectively. Dichlorvos was determined using gas chromatography-mass spectrometry of toluene extracts. The within-assay coefficients of variation were 2 and 5% at 225 and 50 nmol/l (limits of determination), respectively. Since both substances are chemically unstable, the blood was collected by dripping it directly from the vein into 0.74 M phosphoric acid.
authors.affiliation: Department of Clinical Pharmacology, Karolinska Institute, Huddinge University Hospital, Sweden.
authors.forename: T
authors.initials: T
authors.lastname: Villén
authors.forename: Y A
authors.initials: YA
authors.lastname: Abdi
authors.forename: O
authors.initials: O
authors.lastname: Ericsson
authors.forename: L L
authors.initials: LL
authors.lastname: Gustafsson
authors.forename: F
authors.initials: F
authors.lastname: Sjöqvist
mesh_terms: D002849:Chromatography, Gas; D004006:Dichlorvos / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D008401:Gas Chromatography-Mass Spectrometry; D006207:Half-Life; D006801:Humans; D014236:Trichlorfon / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics
chemical_list: D004006:Dichlorvos; D014236:Trichlorfon