The use of fluorescent probes in immunochemistry.
The limitations and advantages of particular dyes for labelling proteins and other biological materials are discussed. Methods available for conjugating dyes to proteins are outlined. Following a discussion of double labelling methods the use of photoactivatable fluorochromes and time resolved fluorescence methodologies are outlined. The reasons for the photoinstability of some fluorochromes are discussed and methods for overcoming the problem are described.
Davidson
R S
RS
Department of Chemistry, City University, London, UK.
Hilchenbach
M M
MM
D000936:Antigen-Antibody Complex / Q000032:analysis; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D011506:Proteins / Q000032:analysis*; D013050:Spectrometry, Fluorescence
D000936:Antigen-Antibody Complex; D005456:Fluorescent Dyes; D011506:Proteins