[The mechanism of photodamage of eye structures. IV. Changes in the reactivity of crystalline SH-groups during UV irradiation].
The kinetics of individual crystalline SH-group modification by DTNB were studied. According to the rates of their interaction with the modifier, the thiol groups in the native protein molecule can be classified as free, accessible, weakly modified and "masked" ones. Denaturation by the detergent (CTAB) caused an increase in the SH-group modification rate. In this case the SH-groups were modified as free and accessible ones. Illumination with UV-light resulted in a decrease in the number of SH-groups, opening of "masked" SH-groups in almost all crystallines except for alpha-crystalline, and essential changes in the SH-group modification rate.
El'chaninov
V V
VV
Fedorovich
I B
IB
D000818:Animals; D002417:Cattle; D003459:Crystallins / Q000378:metabolism / Q000528:radiation effects*; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D005123:Eye / Q000378:metabolism / Q000528:radiation effects*; D007700:Kinetics; D013438:Sulfhydryl Compounds / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects*
D003459:Crystallins; D013438:Sulfhydryl Compounds; D004228:Dithionitrobenzoic Acid
Mekhanizm fotopovrezhdeniia struktur glaza. IV. Izmenenie reaktsionnoĭ sposobnosti SH-grupp belkov khrustalika pri UV-obluchenii.