A serum-free system for culturing human placental trophoblasts.
We have compared hormone production by early gestation and term human placental trophoblasts cultured in Ham's F10 medium containing 10% fetal bovine serum with that by cells cultured in serum-free HB102 medium. Mean daily production of progesterone on Days 3 to 7 was approximately 25% less by both early gestation and term cells cultured in HB102 as compared to Ham's F10, but production was maintained at a stable level for at least 7 d longer than the cells in Ham's. Estradiol production from 10(-6) M dehydroepiandrosterone by both early gestation and term cells was comparable in both media. Human placental lactogen production on Days 3 to 7 was 40% less by cells cultured in HB102. Human chorionic gonadotropin (hCG) output by early gestation cells was also 50% less in HB102 but term cells in HB102 produced twice as much hCG as those in Ham's F10. 3B-Hydroxysteroid dehydrogenase (3BHSD) activity in early gestation and term cells and 11B-hydroxysteroid dehydrogenase (11BHSD) activity of early gestation cultures was comparable in the two media. 11BHSD activity was decreased in the term cultures, and this decrease was more marked in Ham's than in HB102. Sulfatase and aromatase activities in the early gestation cultures were comparable in both media; sulfatase activity was comparable and aromatase activity only 20% less in the term cells cultured in HB102. These results indicate that serum-free HB102 supports differentiated function of human trophoblast cells and is useful for studies of placental activity for as long as 14 d in culture.
Branchaud
C L
CL
McGill University-Montreal Children's Hospital Research Institute, Quebec, Canada.
Goodyer
C G
CG
Guyda
H J
HJ
Lefebvre
Y
Y
D001141:Aromatase / Q000378:metabolism; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000096:biosynthesis; D003470:Culture Media; D004967:Estrogens / Q000096:biosynthesis; D005865:Gestational Age; D006801:Humans; D006913:Hydroxysteroid Dehydrogenases / Q000378:metabolism; D010928:Placental Lactogen / Q000096:biosynthesis; D011374:Progesterone / Q000096:biosynthesis; D014327:Trophoblasts / Q000166:cytology* / Q000378:metabolism
D006063:Chorionic Gonadotropin; D003470:Culture Media; D004967:Estrogens; D011374:Progesterone; D010928:Placental Lactogen; D006913:Hydroxysteroid Dehydrogenases; D001141:Aromatase