The RNA component of RNase P in Schizosaccharomyces species.
In the fission yeast Schizosaccharomyces pombe, the enzyme RNAse P copurifies with two RNAs, K1- and K2-RNA, which are identical except for their termini [1] and which are encoded by a single gene [2]. We have undertaken the cloning of the K-RNA genes in related organisms in order to gain comparative structural information. Because the K-RNA sequence is poorly conserved across species, we have cloned the K-RNA genes in the Schizosaccharomyces species S. malidevorans, S. japonicus, S. versatilis, and S. octosporus. Of the 4 species, only S. octosporus contains a K-RNA gene different from that in S. pombe; the gene diverges by 20%. Based on the two sequences, nuclease protection data and computer analysis, we have proposed a secondary structure model for the K-RNA. Northern analysis shows the K-RNA genes in all four Schizosaccharomyces species to be expressed as two RNAs, as in S. pombe.
Zimmerly
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Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511.
Gamulin
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Burkard
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Söll
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D001483:Base Sequence; D003001:Cloning, Molecular; D004722:Endoribonucleases / Q000737:chemistry* / Q000235:genetics; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D015347:RNA Probes; D012331:RNA, Fungal / Q000737:chemistry*; D043262:Ribonuclease P; D012568:Schizosaccharomyces / Q000201:enzymology / Q000235:genetics*
D015347:RNA Probes; D012331:RNA, Fungal; D004722:Endoribonucleases; D043262:Ribonuclease P