Effect of serum from patients with minimal to mild endometriosis on mouse embryo development in vitro.
Two-cell mouse embryos were cultured at 37 degrees C in 5% CO2, 95% air with a 7.5% serum supplement from patients with minimal to mild endometriosis, (group I, n = 31), tubal factor (group II, n = 33), male factor (group III, n = 17), fetal cord samples (group IV, n = 37), and Ham's F-10 medium (Gibco, Grand Island, NY) without a serum supplement (group V, n = 30). The progression to blastocyst stage (mean percent +/- SE) at 96 hours in groups I, II, III, IV, and V was 29.9% +/- 3.7%, 60.6% +/- 4.9%, 56.2% +/- 5.2%, 61.7% +/- 5.8%, and 63.2% +/- 6.9%, respectively. Serum factors appear to be associated with an inhibition of early embryogenesis, which may explain the decreased fertility rates observed in patients with minimal to mild endometriosis.
Damewood
M D
MD
Department of Gynecology and Obstetrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Hesla
J S
JS
Schlaff
W D
WD
Hubbard
M
M
Gearhart
J D
JD
Rock
J A
JA
D000818:Animals; D001755:Blastocyst / Q000187:drug effects; D001798:Blood Proteins / Q000032:analysis / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media / Q000032:analysis / Q000494:pharmacology; D004622:Embryo, Mammalian / Q000166:cytology / Q000187:drug effects; D005314:Embryonic and Fetal Development / Q000187:drug effects* / Q000502:physiology; D004715:Endometriosis / Q000097:blood*; D005260:Female; D006801:Humans; D051379:Mice; D011247:Pregnancy
D001798:Blood Proteins; D003470:Culture Media