Semi-quantitative determination of H type 1 and type 2 antigens on buccal epithelial cells and in saliva of secretors and non-secretors.
The amount of H antigens on buccal epithelial cells (BEC) of secretors and non-secretors was measured by flow cytometry. H type 1 and H type 2 on BEC were detected with fluorescein-labelled Ulex europaeus lectin, and H type 2 was measured with mouse monoclonal antibody. Between 3.5 and 6 times more H antigen was detected on cells from secretors compared with cells from non-secretors. The level of H type 2 was the same on BEC of secretors and non-secretors. Adsorption of H type 1 antigen from secretor saliva onto non-secretors BEC was demonstrated. A simple and sensitive ELISA method was developed for the determination of H in saliva with biotin-labelled Ulex lectin. This system provides a simple means for measuring the amount of H present in saliva and for the determination of secretor status.
Rahat
A
A
Department of Bacteriology, Medical School, University of Edinburgh, UK.
Stewart
J
J
Blackwell
C C
CC
Weir
D M
DM
D000017:ABO Blood-Group System / Q000276:immunology*; D000327:Adsorption; D002610:Cheek; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D007519:Isoantigens / Q000032:analysis* / Q000378:metabolism; D009061:Mouth Mucosa / Q000166:cytology / Q000276:immunology*; D012463:Saliva / Q000276:immunology* / Q000378:metabolism*
D000017:ABO Blood-Group System; D007519:Isoantigens