title: Trigonal crystals of porcine mitochondrial aspartate aminotransferase.
abstract: Crystals suitable for X-ray analysis of porcine mitochondrial aspartate aminotransferase in the closed conformation were obtained after the apoenzyme was reconstituted with N-5'-phosphopyridoxyl-L-aspartate, an inhibitor in which the cofactor is covalently bound to the substrate. This results in a crystal form that has not been encountered previously in studies of aspartate aminotransferases. The crystals belong to the trigonal space group P3121 (or the enantiomeric P3221) with unit cell dimensions alpha = b = 202.0 A, c = 58.0 A, alpha = beta = 90 degrees, gamma = 120 degrees and contain one dimer in the asymmetric unit.
authors.affiliation: Department of Structural Biology, University of Basel, Switzerland.
authors.forename: T
authors.initials: T
authors.lastname: Izard
authors.forename: B
authors.initials: B
authors.lastname: Fol
authors.forename: R A
authors.initials: RA
authors.lastname: Pauptit
authors.forename: J N
authors.initials: JN
authors.lastname: Jansonius
mesh_terms: D000818:Animals; D001219:Aspartate Aminotransferases / Q000737:chemistry*; D008928:Mitochondria / Q000201:enzymology*; D013552:Swine; D014961:X-Ray Diffraction
chemical_list: D001219:Aspartate Aminotransferases