Protein kinase C inhibitors block insulin and PMA-stimulated hexose transport in isolated rat adipocytes and BC3H-1 myocytes.
Effects of protein kinase C (PKC) inhibitors and "down-regulation" on insulin and PMA-stimulated 2-deoxyglucose transport were determined in isolated rat adipocytes or BC3H-1 myocytes. In both model systems, H-7, sangivamycin, and staurosporine, inhibitors of the catalytic domain of PKC, each effectively blocked insulin and PMA-stimulated hexose uptake at similar concentrations. In the myocytes, staurosporine completely blocked the insulin effect retained post-chronic phorbol myristate acetate (PMA)-induced "down-regulation." These findings indicate (1) that chronic pretreatment with PMA may not lead to a complete loss of PKC activity in the myocyte, and (2) that PKC is involved in insulin-stimulated hexose transport in both isolated rat adipocytes and BC3H-1 myocytes.
Standaert
M L
ML
Veterans' Administration Hospital, Tampa, FL.
Buckley
D J
DJ
Ishizuka
T
T
Hoffman
J M
JM
Cooper
D R
DR
Pollet
R J
RJ
Farese
R V
RV
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000273:Adipose Tissue / Q000166:cytology / Q000201:enzymology*; D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002460:Cell Line; D002469:Cell Separation; D003847:Deoxyglucose / Q000493:pharmacokinetics; D006601:Hexoses / Q000493:pharmacokinetics*; D007328:Insulin / Q000493:pharmacokinetics*; D007546:Isoquinolines / Q000494:pharmacology; D008297:Male; D009132:Muscles / Q000166:cytology / Q000378:metabolism*; D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D011741:Pyrimidine Nucleosides / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D000470:Alkaloids; D006601:Hexoses; D007328:Insulin; D007546:Isoquinolines; D010879:Piperazines; D011741:Pyrimidine Nucleosides; C005699:sangivamycin; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D003847:Deoxyglucose; D011493:Protein Kinase C; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate