title: Quantitative analysis of age-related dendritic changes in medium spiny I (MSI) striatal neurons of C57BL/6N mice.
abstract: Our study used quantitative morphometric analysis and Golgi staining methods to evaluate postnatal changes in the dendritic architecture of MSI neurons of the striatum between 1 and 30 months of age. Morphological changes and chronological age were also correlated with functional testing in order to identify subpopulations of aged mice with dendritic alterations that may be more characteristic of a motor deficit rather than the normal aging process. We found that the overall size of the dendritic arbor of MSI neurons in the rostral striatum remained stable with age, while caudal MSI neurons exhibited a significant elongation of terminal dendritic segments between 25 and 30 months of age. In addition, our correlation analysis of motor performance and chronological age found that neither striatal-motor deficits nor their associated anatomical correlates were inevitable consequences of senescence but were characteristic for a select subpopulation of aged mice with striatal-motor deficits. We found that mice that tested poorly on the balance rod had a significant increase in the number of MSI neurons with small dendritic arbors in various stages of atrophic degeneration. Conversely, 30-month-old mice that had no functional impairment showed no significant change in the number of neurons with atrophic dendrites. These data reinforce the premise that the correlation of structure and function plays an important role in the analysis of an aging population since data may vary based on the number of functionally impaired or unimpaired mice that make up an experimental group.
authors.affiliation: Andrus Gerontology Center, University of Southern California, Los Angeles 90089-0191.
authors.forename: T H
authors.initials: TH
authors.lastname: McNeill
authors.forename: L L
authors.initials: LL
authors.lastname: Koek
authors.forename: S A
authors.initials: SA
authors.lastname: Brown
authors.forename: J A
authors.initials: JA
authors.lastname: Rafols
mesh_terms: D000367:Age Factors; D000375:Aging / Q000473:pathology* / Q000502:physiology; D000818:Animals; D001284:Atrophy; D003342:Corpus Striatum / Q000737:chemistry / Q000473:pathology* / Q000502:physiology; D003712:Dendrites / Q000737:chemistry / Q000473:pathology* / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000737:chemistry / Q000473:pathology* / Q000502:physiology