cdc2 gene expression at the G1 to S transition in human T lymphocytes.
The product of the cdc2 gene, designated p34cdc2, is a serine-threonine protein kinase that controls entry of eukaryotic cells into mitosis. Freshly isolated human T lymphocytes (G0 phase) were found to have very low amounts of p34cdc2 and cdc2 messenger RNA. Expression of cdc2 increased 18 to 24 hours after exposure of T cells to phytohemagglutinin, coincident with the G1 to S transition. Antisense oligodeoxynucleotides could reduce the increase in cdc2 expression and inhibited DNA synthesis, but had no effect on several early and mid-G1 events, including blastogenesis and expression of interleukin-2 receptors, transferrin receptors, c-myb, and c-myc. Induction of cdc2 required prior induction of c-myb and c-myc. These results suggest that cdc2 induction is part of an orderly sequence of events that occurs at the G1 to S transition in T cells.
Furukawa
Y
Y
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA 02115.
Piwnica-Worms
H
H
Ernst
T J
TJ
Kanakura
Y
Y
Griffin
J D
JD
D001483:Base Sequence; D015152:Blotting, Northern; D016203:CDC2 Protein Kinase / Q000096:biosynthesis / Q000235:genetics*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D005434:Flow Cytometry; D016193:G1 Phase; D005786:Gene Expression Regulation; D016161:Genes, Retinoblastoma; D016259:Genes, myc; D006801:Humans; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D010766:Phosphorylation; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins / Q000235:genetics; D020598:Proto-Oncogene Proteins c-myb; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D016196:S Phase; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism
D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D012333:RNA, Messenger; D004247:DNA; D016203:CDC2 Protein Kinase