[The effect of peripheral blood leukocyte depletion on reperfusion injury of warm ischemic lung].
To clarify the role of leukocytes in reperfusion injury of preserved lung for lung transplantation, leukocyte depletion was performed and lung tissue generation of oxygen metabolites and histological evaluation were investigated.


METHODS
12 mongrel dogs were utilized, divided into 2 groups. In group A, hilar-stripped left lung was submitted to 2 hour warm ischemia and 2 hour reperfusion. Blood filtration was performed three times using leukocyte filter. In group B, warm ischemia and reperfusion were performed without filtration. Lipid peroxidation products of lung tissue were measured by thiobarbituric acid assay. Tissue damage was estimated by wet/dry ratio and histological analysis. Results 1). Peripheral blood counts revealed that effective leukocyte depletion was obtained and leukocyte efflux from reperfused lung was observed in group A. 2) Significant increase of lung tissue oxygen metabolite generation after reperfusion was observed in group B, while no significant fluctuate of tissue MDA titers was noted in group A. Tissue damage estimated by histological analysis and wet/dry ratio were considered to be controlled in group A compared with group B. In conclusion, leukocyte depletion by in vivo use of leukocyte filter was effective for reperfusion injury of warm ischemic lung and oxygen metabolites may play a role in these process.
Ide
H
H
Department of Cardiovascular Surgery, Jichi Omiya Medical Center, Japan.
Ino
T
T
Hasegawa
T
T
Matsumoto
H
H
D000818:Animals; D004285:Dogs; D005374:Filtration; D006979:Hyperthermia, Induced; D007937:Leukapheresis / Q000379:methods; D007958:Leukocyte Count; D008168:Lung / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D008315:Malondialdehyde / Q000378:metabolism; D009926:Organ Preservation; D010100:Oxygen / Q000378:metabolism; D015427:Reperfusion Injury / Q000097:blood / Q000378:metabolism / Q000517:prevention & control*
D008315:Malondialdehyde; D010100:Oxygen