Different effects of TPA on two skin-derived cell lines: murine (HEL-30) and human (NCTC) epidermal cells.
12-O-tetradecanoylphorbol-13-acetate (TPA) caused a rapid activation of protein kinase C in a murine (HEL-30) and in a human (NCTC) epidermal cell line. In HEL-30 cells, protein kinase C activation is followed by ornithine decarboxylase stimulation and cell proliferation, events inhibited by H-7, a specific inhibitor of protein kinase C. TPA in NCTC cells inhibited the basal ornithine decarboxylase activity and cell growth, whereas H-7 did not modify TPA effect. The response of NCTC cells was not due to direct toxicity of TPA. These data confirm that in murine epidermal cells, the proliferation induced by TPA is mediated by protein kinase C, whereas in a human skin-derived cell line these events are not or inversely associated.
Corsini
E
E
Institute of Pharmacological Sciences, University of Milan, Italy.
Restani
P
P
Galli
C L
CL
Marinovich
M
M
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D051379:Mice; D009955:Ornithine Decarboxylase / Q000378:metabolism; D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D011955:Receptors, Drug / Q000187:drug effects; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D013045:Species Specificity; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D007546:Isoquinolines; D010879:Piperazines; D011955:Receptors, Drug; C083141:phorbol ester binding protein; C027776:phorbol ester receptor; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D007770:L-Lactate Dehydrogenase; D011493:Protein Kinase C; D009955:Ornithine Decarboxylase; D013755:Tetradecanoylphorbol Acetate