Sexual response of Saccharomyces cerevisiae: phosphorylation of yeast glyoxalase I by a cell extract of mating factor-treated cells.
The phosphorylation of glyoxalase I was observed when the phosphatase-treated enzyme was incubated in the presence of [gamma-32P]ATP and a cell extract prepared from alpha-type yeast cells which had been treated with the culture supernatant of a-type yeast cells. The phosphorylated protein was identified as glyoxalase I by using anti-glyoxalase I rabbit immunoglobulin G.
Inoue
Y
Y
Research Institute for Food Science, Kyoto University.
Choi
B Y
BY
Murata
K
K
Kimura
A
A
D001487:Basidiomycota / Q000201:enzymology / Q000235:genetics; D005800:Genes, Fungal; D007074:Immunoglobulin G / Q000276:immunology; D007791:Lactoylglutathione Lyase / Q000235:genetics* / Q000378:metabolism; D008970:Molecular Weight; D010766:Phosphorylation; D011233:Precipitin Tests; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013050:Spectrometry, Fluorescence
D007074:Immunoglobulin G; D007791:Lactoylglutathione Lyase