title: [Development of a system of intragenic mapping for molecular genetic analysis of mutations in the gene LYS2 of Saccharomyces yeasts].
abstract: The collection of overlapping lys2 deletions (five in the chromosomal and seven in the plasmid LYS2 gene) is constructed in this work. The deletions overlap the whole coding region of the gene and provide the system for intragenic recombinational mapping of lys2 mutations in one of 14 controlled regions. A portion of these regions can be correlated with the regions on the physical map of LYS2. Mutations in two regions can be easily cloned. The system constructed gives the possibility for the study of intragenic and molecular specificity of mutagenesis.
authors.forename: V N
authors.initials: VN
authors.lastname: Noskov
authors.forename: M G
authors.initials: MG
authors.lastname: Tarutina
authors.forename: Iu I
authors.initials: IuI
authors.lastname: Pavlov
authors.forename: V N
authors.initials: VN
authors.lastname: Kulikov
authors.forename: M V
authors.initials: MV
authors.lastname: Trofimova
authors.forename: A V
authors.initials: AV
authors.lastname: Gorbacheva
authors.forename: Iu O
authors.initials: IuO
authors.lastname: Chernov
authors.forename: K V
authors.initials: KV
authors.lastname: Sasnauskas
authors.forename: M A
authors.initials: MA
authors.lastname: Neĭstat
authors.forename: I I
authors.initials: II
authors.lastname: Tolstorukov
mesh_terms: D000483:Alleles; D002872:Chromosome Deletion; D015825:Chromosomes, Fungal; D005800:Genes, Fungal; D005818:Genetic Engineering; D009154:Mutation; D010957:Plasmids; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*
vernacular_title: Razrabotka sistemy vnutrigennogo kartirovaniia dlia molekuliarno- geneticheskogo analiza mutatsiĭ v gene LYS2 u drozhzheĭ sakharomitsetov.