Effect of local anaesthetics on mitochondrial membrane potential in living cells.
Using the laser dye rhodamine 123, we demonstrated that local anaesthetics can reach mitochondria in cell culture and reversibly decrease, or even collapse, their transmembrane potential. This effect is highly dependent on the lipid-solubility of the local anaesthetic and can be facilitated by the presence of a lipophilic anion.
Grouselle
M
M
Centre de Recherche Paul Pascal-CNRS, Château Brivazac, France.
Tueux
O
O
Dabadie
P
P
Georgescaud
D
D
Mazat
J P
JP
D000779:Anesthetics, Local / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D002045:Bupivacaine / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D005041:Etidocaine / Q000737:chemistry / Q000494:pharmacology; D005347:Fibroblasts / Q000648:ultrastructure; D005456:Fluorescent Dyes; D007425:Intracellular Membranes / Q000187:drug effects / Q000502:physiology*; D008012:Lidocaine / Q000737:chemistry / Q000494:pharmacology; D008563:Membrane Lipids; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology*; D020112:Rhodamine 123; D012235:Rhodamines; D012995:Solubility
D000779:Anesthetics, Local; D005456:Fluorescent Dyes; D008563:Membrane Lipids; D012235:Rhodamines; D020112:Rhodamine 123; D008012:Lidocaine; D005041:Etidocaine; D002045:Bupivacaine