Evaluation of monoclonal antibody F9-4 as immunological probe for Leptospira interrogans.
I assayed the lack of reactivity of monoclonal antibody (MAb) F9-4 with nonpathogenic leptospires. Of 47 saprophytic strains tested, 46 did not react in an enzyme immunoassay and 1 was recognized by MAb F9-4, as usually reported with pathogenic Leptospira strains. On the other hand, the MAb did not react with one pathogenic strain, thus showing that the ability of MAb F9-4 to discriminate between pathogenic and nonpathogenic leptospires is not absolute.
Cinco
M
M
Istituto di Microbiologia, Università di Trieste, Italy.
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D015415:Biomarkers; D005069:Evaluation Studies as Topic; D006801:Humans; D007921:Leptospira interrogans / Q000145:classification / Q000276:immunology* / Q000472:pathogenicity; D013045:Species Specificity; D014774:Virulence / Q000276:immunology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D015415:Biomarkers