title: Bioluminescent flow sensor for the determination of L-(+)-lactate.
abstract: The amount of L-lactate in biological fluids (serum, plasma and cerebrospinal fluid) was determined by monitoring the reduced form of nicotinamide adenine dinucleotide (NADH) produced by immobilised lactate dehydrogenase (LDH), with bacterial bioluminescent enzymes immobilised on a separate nylon coil. The LDH catalysed the reaction of L-lactate with NAD; this reaction took place in a nylon coil that preceded the coil for the bioluminescent detection. The co-immobilisation of alanine aminotransferase (ALT) with LDH improved the lactate transformation by 117-183%. The response was linear from 0.1 to 50 micron mol l(-1) at 25 degrees C for the LDH - ALT reactor. The intra- and inter-assay coefficients of variation were less than 5% and the recoveries ranged from 93 to 106%. The results agreed well with those obtained with a spectrophotometric method and with the normal reference values.
authors.affiliation: Institute of Chemical Sciences, University of Bologna, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Girotti
authors.forename: B
authors.initials: B
authors.lastname: Grigolo
authors.forename: E
authors.initials: E
authors.lastname: Ferri
authors.forename: S
authors.initials: S
authors.lastname: Ghini
authors.forename: G
authors.initials: G
authors.lastname: Carrea
authors.forename: R
authors.initials: R
authors.lastname: Bovara
authors.forename: A
authors.initials: A
authors.lastname: Roda
authors.forename: R
authors.initials: R
authors.lastname: Motta
authors.forename: R
authors.initials: R
authors.lastname: Petilino
mesh_terms: D000410:Alanine Transaminase; D015374:Biosensing Techniques; D001826:Body Fluids; D004355:Drug Stability; D004800:Enzymes, Immobilized; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007773:Lactates / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid; D019344:Lactic Acid; D049449:Luminescence; D009243:NAD / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D007773:Lactates; D009243:NAD; D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase; D000410:Alanine Transaminase