title: Electrophilic levuglandin E2-protein adducts bind glycine: a model for protein crosslinking.
abstract: Prevention by glycine of protein crosslinking which accompanies binding of levuglandin E2 (LGE2) is shown to involve binding of glycine with the protein-LGE2 adduct. With ovalbumin, the LGE2 adduct initially binds nearly 2 equivalents of glycine, but the capacity to bind glycine decreases with time reflecting a competition, inter alia, with crosslinking.
authors.affiliation: Department of Chemistry, Case Western Reserve University, Cleveland, Ohio 44106-2699.
authors.forename: M R
authors.initials: MR
authors.lastname: Jirousek
authors.forename: K K
authors.initials: KK
authors.lastname: Murthi
authors.forename: R G
authors.initials: RG
authors.lastname: Salomon
mesh_terms: D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D005998:Glycine / Q000378:metabolism*; D007700:Kinetics; D010047:Ovalbumin / Q000378:metabolism*; D011458:Prostaglandins E / Q000378:metabolism
chemical_list: D003432:Cross-Linking Reagents; D011458:Prostaglandins E; D010047:Ovalbumin; C047684:levuglandin E2; D005998:Glycine