Comparative sequence of the helper component (HC) region of potato virus Y and a HC-defective strain, potato virus C.
Potato virus C (PVC), a non-aphid transmissible strain of potato virus Y (PVY), was found to code for a protein (PVC-HC) which is similar in molecular weight and immunological reactivity to the helper component protein of PVY (PVY-HC). PVC-HC, however, was inactive with respect to its ability to effect aphid transmission of either PVC or PVY. The 5'-terminal 2.7-kb regions of PVC and PVY were sequenced. Within the HC region there was 92% nucleotide homology between the two strains; comparison of the derived amino acid sequences revealed 24 amino acid differences. Comparison of the PVC-HC sequence with that of five potyviruses revealed 2 amino acid changes which were specific to PVC-HC. These amino acids are prime targets for mutational analysis of HC activity.
Thornbury
D W
DW
Department of Plant Pathology, University of Kentucky, Lexington 40546.
Patterson
C A
CA
Dessens
J T
JT
Pirone
T P
TP
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000235:genetics*; D004279:DNA, Viral / Q000737:chemistry; D005796:Genes; D008969:Molecular Sequence Data; D009154:Mutation; D010942:Plant Viruses / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; D014764:Viral Proteins; D003546:Cysteine Endopeptidases; C072775:HC-Pro protein, potyvirus