Growth stimulation by androgens, glucocorticoids or fibroblast growth factors and the blocking of the stimulated growth by antibody against basic fibroblast growth factor in protein-free culture of Shionogi carcinoma 115 cells.
Shionogi carcinoma 115 (SC115) has been accepted for 20 years as an androgen-responsive mouse mammary tumor. We have established an androgen-dependent cloned cell line (SC-3) from a SC115 tumor. In a serum-free medium, testosterone (T) or fibroblast growth factors (FGFs) markedly stimulate the growth of SC-3 cells, and the T-induced growth was shown to be mediated through FGF-like peptide(s) in an autocrine mechanism. Since we used the serum-free culture including 0.1% bovine serum albumin (BSA), a partially serum-containing condition, putative roles of BSA- or serum-borne growth factors in growth stimulation of autocrine production of FGF-like peptide(s) could not be excluded. This paper reports findings performed in a protein-free medium including plating [Ham's F-12:MEM (1:1; v/v)]. In the protein-free culture, the growth of SC-3 cells was significantly stimulated by the addition of greater than or equal to 10(-10) M T (up to 20-fold), greater than or equal to 10(-7) M dexamethasone (Dex; up to 7-fold) or greater than or equal to 1 ng/ml basic (b) or acidic FGF (up to 10-fold); other various growth factors had no such effects. Furthermore, DNA synthesis of SC-3 cells induced by T, Dex or bFGF was similarly and markedly inhibited by bFGF neutralizing antibody IgG. Therefore, the present findings seem to demonstrate that androgens or high levels of glucocorticoids induce the production and secretion of FGF-like peptide(s) from SC-3 cells for their growth even in the absence of additional support by other factors.
Tanaka
A
A
Department of Pathology, Osaka University Medical School, Japan.
Matsumoto
K
K
Nishizawa
Y
Y
Lu
J
J
Yamanishi
H
H
Maeyama
M
M
Nonomura
N
N
Uchida
N
N
Sato
B
B
D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000187:drug effects* / Q000276:immunology; D003907:Dexamethasone / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D005938:Glucocorticoids / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D013739:Testosterone / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000648:ultrastructure
D000728:Androgens; D005938:Glucocorticoids; D007074:Immunoglobulin G; D013739:Testosterone; D005346:Fibroblast Growth Factors; D003907:Dexamethasone; D013936:Thymidine