title: Interleukin-4 regulates mRNA accumulation of macrophage-colony stimulating factor by fibroblasts: synergism with interleukin-1 beta.
abstract: We demonstrate that macrophage-colony stimulating factor (M-CSF) is expressed in human fibroblasts at the mRNA and protein level. Following activation with both interleukin (IL)-1 beta and IL-4, fibroblasts synthesized M-CSF transcripts detectable by Northern blot analysis with peak expression occurring at 8 h and 12 h, respectively. Exposure of fibroblasts to both cytokines resulted in M-CSF protein release at 60 h of c. 500 U/ml (for IL-1 beta) and 1000 U/ml (for IL-4), relative to a control preparation of recombinant human M-CSF in a murine bone marrow colony assay. Both interleukins synergized to enhance M-CSF mRNA accumulation and their ability to induce M-CSF transcripts could be abolished by treatment with specific neutralizing antibodies. These observations provide support for the idea that fibroblasts may control monocyte/macrophage development and function, and that IL-1 beta and IL-4 are involved in the regulation of this process.
authors.affiliation: Department of Internal Medicine I, Albert-Ludwigs-Universität, Freiburg, F.R.G.
authors.forename: R
authors.initials: R
authors.lastname: Henschler
authors.forename: L
authors.initials: L
authors.lastname: Mantovani
authors.forename: W
authors.initials: W
authors.lastname: Oster
authors.forename: M
authors.initials: M
authors.lastname: Lübbert
authors.forename: A
authors.initials: A
authors.lastname: Lindemann
authors.forename: R
authors.initials: R
authors.lastname: Mertelsmann
authors.forename: F
authors.initials: F
authors.lastname: Herrmann
mesh_terms: D015152:Blotting, Northern; D002478:Cells, Cultured; D004357:Drug Synergism; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000502:physiology*; D015847:Interleukin-4 / Q000502:physiology*; D016173:Macrophage Colony-Stimulating Factor / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000502:physiology
chemical_list: D007375:Interleukin-1; D012333:RNA, Messenger; D011994:Recombinant Proteins; D015847:Interleukin-4; D016173:Macrophage Colony-Stimulating Factor