title: Biosynthesis of cyclic 2,3-diphosphoglycerate. Isolation and characterization of 2-phosphoglycerate kinase and cyclic 2,3-diphosphoglycerate synthetase from Methanothermus fervidus.
abstract: Starting from 2-phosphoglycerate the biosynthesis of cDPG comprises two steps: (i) the phosphorylation of 2-phosphoglycerate to 2,3-diphosphoglycerate and (ii) the intramolecular cyclization to cyclic 2,3-diphosphoglycerate. The involved enzymes, 2-phosphoglycerate kinase and cyclic 2,3-diphosphoglycerate synthetase, were purified form Methanothermus fervidus. Their molecular and catalytic properties were characterized.
authors.affiliation: Max-Planck-Institut für Biochemie, Martinsried, FRG.
authors.forename: A
authors.initials: A
authors.lastname: Lehmacher
authors.forename: A B
authors.initials: AB
authors.lastname: Vogt
authors.forename: R
authors.initials: R
authors.lastname: Hensel
mesh_terms: D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate / Q000378:metabolism; D019843:Archaeal Proteins; D004163:Diphosphoglyceric Acids / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D019605:Euryarchaeota / Q000201:enzymology*; D005988:Glyceric Acids / Q000378:metabolism; D007700:Kinetics; D008190:Lyases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D046911:Macromolecular Substances; D008970:Molecular Weight; D010735:Phosphoglycerate Kinase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D019761:Phosphorus-Oxygen Lyases; D011188:Potassium / Q000494:pharmacology
chemical_list: D019843:Archaeal Proteins; D004163:Diphosphoglyceric Acids; D005988:Glyceric Acids; D046911:Macromolecular Substances; D019794:2,3-Diphosphoglycerate; C008885:2-phosphoglycerate; C039905:cyclic-2,3-diphosphoglycerate; D000255:Adenosine Triphosphate; D010735:Phosphoglycerate Kinase; D008190:Lyases; D019761:Phosphorus-Oxygen Lyases; C066348:cdpgs protein, Methanothermus fervidus; D011188:Potassium