Production of interleukin 2 and expression of interleukin 2 receptors by pony peripheral blood lymphocytes after stimulation with a soluble fraction of Trypanosoma evansi.
Pony peripheral blood lymphocytes (PBL) were stimulated with a soluble fraction of Trypanosoma (T.) evansi (SF). As determined by 3H-thymidine incorporation, the cells underwent a proliferative response and were able to: a) produce a factor having the biological activities of interleukin 2 (IL-2) since their supernatants could support the in vitro growth of pony PBL stimulated with concanavalin A (Con A-blasts); b) undergo a further proliferative response when incubated in short term cultures with SF, human recombinant IL-2 (hrIL-2), or both c) bind specifically radiolabelled hrIL-2 (125I-hrIL-2). The date described here indicate that a soluble fraction of T. evansi stimulated pony PBL which subsequently produced IL-2 and expressed IL-2 receptors (IL-2R).
Ahmed
J S
JS
Institut für Parasitologie und Tropenveterinärmedizin der Freien Universität, Berlin.
Schmid
G
G
Hörchner
F
F
D000818:Animals; D002478:Cells, Cultured; D006736:Horses; D007376:Interleukin-2 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis*; D014345:Trypanosoma / Q000276:immunology*
D007376:Interleukin-2; D015375:Receptors, Interleukin-2