Effective pretreatment by cysteine proteinase inhibitor for improved analysis of protein components of trematodes on SDS-PAGE.
Since Fasciola sp. contained proteolytic enzyme(s), it was confirmed that degradation took place in protein components in extracts of the liver flukes, which resulted in lack of clarity of sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Degradation was shown to occur mostly during a heating process of the extract samples. The proteolytic activity in the extracts was completely blocked and electrophoretic patterns were improved only by the use of cysteine proteinase inhibitor N-[N-(L-3-trans-carboxyoxiran-2-carbonyl)-L-leucyl]-agmatine (E-64). Great improvement was also noted in electrophoretic patterns of the extracts of other trematodes, such as Paragonimus westermani, P. miyazakii and Clonorchis sinesis, when their extracts were treated with E-64.
Itoh
M
M
Department of Medical Zoology, Nagoya City University Medical School, Japan.
Sato
S
S
Moriyama
A
A
Sasaki
M
M
D000818:Animals; D003004:Clonorchis sinensis / Q000032:analysis* / Q000187:drug effects; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D005209:Fasciola / Q000032:analysis* / Q000187:drug effects; D015801:Helminth Proteins / Q000032:analysis*; D010238:Paragonimus / Q000032:analysis* / Q000187:drug effects
D015853:Cysteine Proteinase Inhibitors; D015801:Helminth Proteins