title: Application of a rapid microplaque assay for determination of human immunodeficiency virus neutralizing antibody titers.
abstract: To perform a human immunodeficiency virus (HIV) plaque assay in nonadherent host cells, we developed a novel technique in which HIV-infected MT-2 cells were formed into monolayers by centrifugation through molten agarose. Infection, formation of cell monolayers, and enumeration of plaques all took place in 96-well microtiter plates. When this process was preceded by 18 h of incubation of HIV with patient serum samples, neutralizing antibody titers between 1:10 and 1:5,000 could be accurately determined in patient serum samples. In addition to the determination of neutralizing antibody titers (with the use of various serum dilutions and a constant virus concentration), neutralization indices could also be determined with different virus dilutions and a single dilution of patient serum.
authors.affiliation: Viral and Rickettsial Disease Laboratory, California Department of Health Services, Berkeley 94704.
authors.forename: C V
authors.initials: CV
authors.lastname: Hanson
authors.forename: L
authors.initials: L
authors.lastname: Crawford-Miksza
authors.forename: H W
authors.initials: HW
authors.lastname: Sheppard
mesh_terms: D002460:Cell Line; D005069:Evaluation Studies as Topic; D015483:HIV Antibodies / Q000032:analysis*; D015497:HIV-1 / Q000254:growth & development; D006801:Humans; D009500:Neutralization Tests; D010948:Viral Plaque Assay / Q000379:methods*
chemical_list: D015483:HIV Antibodies