title: Rat DNA polymerase beta gene can join in excision repair of Escherichia coli.
abstract: Though DNA polymerase I (poll) of Escherichia (E.) coli is understood to play a role in repair synthesis of excision repair, it is still obscure whether DNA polymerase beta (pol beta) plays a similar role in eukaryotic cells. To estimate the role of pol beta in excision repair processes, we inserted the rat pol beta gene into several mutant E. coli defective in a diverse set of enzymatic activities of poll. UV resistance was seen only when the 5'----3' exonuclease (exo) activity of poll molecules remained. Therefore it is suggested that 5'----3' exo activity as well as pol beta activity are essential for repair synthesis of excision repair in eukaryotic cells.
authors.affiliation: Department of Biology, Nara Medical University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ohnishi
authors.forename: S
authors.initials: S
authors.lastname: Yuba
authors.forename: T
authors.initials: T
authors.lastname: Date
authors.forename: H
authors.initials: H
authors.lastname: Utsumi
authors.forename: A
authors.initials: A
authors.lastname: Matsukage
mesh_terms: D000818:Animals; D004249:DNA Damage; D004256:DNA Polymerase I / Q000235:genetics* / Q000378:metabolism; D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D009154:Mutation; D010957:Plasmids; D051381:Rats; D015183:Restriction Mapping; D014162:Transfection; D014169:Transformation, Bacterial; D014466:Ultraviolet Rays
chemical_list: D004269:DNA, Bacterial; D004256:DNA Polymerase I