Antisense RNA production in transgenic mice.
There are many reports of antisense inhibition of gene expression in cultured cells. We have generated four strains of transgenic mice expressing antisense hypoxanthine guanine phosphoribosyltransferase (HPRT) RNA in brain, or heart and liver, or all three organs. In the brain of one strain, the level of antisense RNA in the different brain regions roughly correlates with the degree of inhibition of the native HPRT mRNA in those same regions. Despite this decrease of up to 60% of endogenous HPRT mRNA, no reproducible reduction in HPRT activity has been observed. Possible reasons for the differences between the effectiveness of antisense inhibition in cultured cells and transgenic animals are discussed.
Munir
M I
MI
Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030.
Rossiter
B J
BJ
Caskey
C T
CT
D000818:Animals; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics* / Q000378:metabolism; D066298:In Vitro Techniques; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D009928:Organ Specificity; D010375:Pedigree; D016372:RNA, Antisense / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000378:metabolism
D016372:RNA, Antisense; D012333:RNA, Messenger; D007041:Hypoxanthine Phosphoribosyltransferase