Messenger-dependent action of guanylylimidodiphosphate and translation inhibitors on rat brain polypeptide synthesis.
The effects of the Guanosine 5'-triphosphate analog guanylylimidodiphosphate and several antibiotics on protein synthesis in a cell-free system from rat brain were investigated as a function of the template used. Polypeptide chain elongation was promoted by the analog only in the system coded by the endogenous template but not when coded by polyuridylic acid. The effects of the antibiotics were also dependent on the template used. This suggest that the template conditioned the ribosomal conformation which determine the inhibitory effect of the antibiotics.
Muñoz
R
R
Departamento de Bioquímica, Biología Molecular y Fisiología, Facultad de Ciencias, Universidad de Valladolid, Spain.
Ferreras
J M
JM
Iglesias
R
R
Merino
M J
MJ
Girbes
T
T
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001921:Brain / Q000378:metabolism*; D002474:Cell-Free System; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology*; D007700:Kinetics; D015636:Magnesium Chloride / Q000494:pharmacology; D010452:Peptide Biosynthesis; D010441:Peptide Chain Elongation, Translational / Q000187:drug effects*; D014176:Protein Biosynthesis / Q000187:drug effects*; D051381:Rats; D013698:Templates, Genetic
D000900:Anti-Bacterial Agents; D015636:Magnesium Chloride; D006165:Guanylyl Imidodiphosphate