title: Affinity immunoblotting detection of serum monoclonal immunoglobulins reactive with glycosphingolipids.
abstract: The characterization of serum monoclonal IgM reactive with glycosphingolipid present in the nervous system is of major importance in patients with certain neuropathies. A rapid, sensitive and specific one-step method which permits such a characterization is described. Serum immunoglobulins separated by high resolution agarose electrophoresis are transferred to affinity filters (nitrocellulose sheets coated with glycolipids), and revealed with enzyme-tagged monospecific anti-immunoglobulin antibodies.
authors.affiliation: Laboratory of Immunology, University Hospital, Limoges, France.
authors.forename: M O
authors.initials: MO
authors.lastname: Jauberteau
authors.forename: J M
authors.initials: JM
authors.lastname: Cook
authors.forename: M
authors.initials: M
authors.lastname: Drouet
authors.forename: J L
authors.initials: JL
authors.lastname: Preud'homme
mesh_terms: D000911:Antibodies, Monoclonal / Q000097:blood* / Q000276:immunology; D001927:Brain Diseases / Q000097:blood*; D003711:Demyelinating Diseases / Q000276:immunology; D004587:Electrophoresis, Agar Gel; D006028:Glycosphingolipids / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D007075:Immunoglobulin M / Q000032:analysis* / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D006028:Glycosphingolipids; D007075:Immunoglobulin M